LOCUS       DAJB01000001           18156 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_5689, whole genome shotgun sequence.
ACCESSION   DAJB01000001 DAJB01000000
VERSION     DAJB01000001.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18156)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_5689"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3987 a         5015 c         5110 g         4044 t
ORIGIN      
        1 gggtctcctg cctgattatt atgactgatc cagagtttgg aggcatagag tgagcggact
       61 tgatatgcga agcagcgaac gggagtgcag tgcccgatgg gagcacgcgt tcgaggctga
      121 tccggctgag aaggagaagt actacctgac cgtggcattc ccgtatccaa gcggggcgat
      181 gcacgtcggg cacgggcgga cctacatcgt tccggatgtc gtcgcccggt accagcggat
      241 gagggggaag caggtccttt tcccgatggc gttccacgtc accggcactc cggtcatcgg
      301 gatatcgaag aggatcgcaa acggcgacgc gaagacggtc gggctgtacc gtgacctcta
      361 ccgggtgccg caggatatcc tcgaccggtt catcaacccg atggagattg tccggcactt
      421 ctcggaggag taccggcgcg tgatgcagaa gtgcgggctc tcgatcgact ggcggcgccg
      481 gttcatcacc gtcgaccccc agtacagcaa gttcatcgag tggcagtatg cgcacctgca
      541 cgaggacgag cacgtcgtca agggtgccca cccggtcaag tactgtcccc agtgcgagaa
      601 ccctgtcggc gaccacgacc tgctcgaggg cgagaaggcc gagatcatca agttcaccct
      661 ggtggtcttc tcctggggcg atgccctggt cccgtgcgca acgctccggc ccgagacggt
      721 ctacggcgtg acgaacctct gggtcaatcc ggagaccacc tacgtacggg tgacgctcga
      781 cggcaaagag tggatcgtaa gccgggaggc ggcagcgaaa ctcgccctgc aggaccacga
      841 ggttcgtgcg ggagaggaga tccccgggac ggcgctcatc gaccagacgg tctctcaccc
      901 cttaagcggc gaggtccccg ttctgccggc atcgttcgtg gacccggata tggggaccgg
      961 gatcgtcatg agcgttcccg cccacgcacc cttcgactac atcgcgctcc gcgacctgca
     1021 gcagcagggg aagtacacgg ccatccggcc ggtcccgctc atctccgtcg agggctacgg
     1081 cgagatcccg gcaaaggatg cagtcgaacg ggcgggcatc cgcgaccaga acgatccggg
     1141 aatggaggcg ctcacccagg aggtctacag cgccgagttc tcccgcggga aggtctttga
     1201 caagtacggc ggaaagccgg tgcgggaggc ccgggacgac gtggcggtgc tgatgatgga
     1261 gcgctacggc tccatcccga tgtatgagtt cgacaaccgc caggtgatct gccggtgcgg
     1321 cagcagggtc tttgtgaaga tcctccatga ccagtggttc ctggagtaca gcgacccgtg
     1381 ctggaaggag caggtgaaga cccagctcga tgagatggcg ctcgttcccc ccgaggtccg
     1441 gaccgagttc aaccggaccg tcgactggtt aaaggactgg gcctgcaccc ggcgggtggg
     1501 gctcggcacg aagctcccct gggacccgac ctggatcatc gagcccctct ccgactcgac
     1561 gatctacatg gcctactaca cgatcgctca ccacttaaat gccatagcgc cggaaaaact
     1621 gacgccggac gtctttgagt acatcttcaa gggcgagggg aacccgacca cggtcgaccg
     1681 cgagaccctc gaccggatcc ggagcgagtt cctctactgg tatccctacg attaccggtt
     1741 ctcggcaaag gacctcatct cgaaccatct cacgttccag ctcttccacc accgggctat
     1801 cttcccggca aaactgcagc cgaagggcat ggtggtcttc ggcatgggcc tcttgaacgg
     1861 ggcgaagatg tcgtcgagca aaggaaacgt cttcctgctc gaggatgccc tggaggagtt
     1921 cggcgccgat acggtcagga tgttccttgt cgggagcgca gagccctggc aggactttga
     1981 ctggaggaac gagctcgtct cgtcgaccag aaagcagatc gagcggttct ggaccacggt
     2041 cgaggaggca aagcaggcca aaggagcctc cgacatcgat gcctggctcg cgagcaggct
     2101 ccagcaccgc atcgagagcg tcaccgcggc gcttgattcg ttccagaccc gacaggcact
     2161 gcaggaggcg tttttcgggg tggaggcgga cctgaagtgg taccgccgcc gcctgcccga
     2221 gggcgcaacc ggcggggccg tgatgcagga cctctgccgc acctgggtgc ggctgcttgc
     2281 cccgatcatc cccttcacct gcgagacgct ctggagcgag atcggcgggg aaggcatggt
     2341 ctccttcgcc tcctggccgg aggtggacga ggcccgggtc aacccggatg tcgagctcgc
     2401 cgaagaactc ctctcaagga cggtcgagga tatcgagtcg atcgtcaaac tcatcccgat
     2461 cgagccgaag tccatcagcc tcttcgtcgc tccggcctgg aagcacgagg ccttccggat
     2521 catcgcagcc tcgaccgata agacgagggt ggtgcgggag atcatgcaga acgaggagat
     2581 gcgcaagcgc gggcgcgagg cgacggacgc ggcaaagcag atcacgaaac tcgtactgaa
     2641 attgccgccc gaactcgtga agcagctcgc agggtcgtcc cccgacgagc aggcagtcct
     2701 cgaaggcgct cgatcgttcc tggagcacga gttcggcgtg ccggtgacgg tcaggaacgc
     2761 cgaggagagc acgcacccga aggcctcggg ggccctgccc ttcaagccgg cgattgtgat
     2821 cgagtaggat actcactttt ttttacgctt gttttttgag cacggttttt aagcccccct
     2881 cccccacgtc cctcgtatct ccatggagag tcggcggtgg agccggggca gaggatctgg
     2941 tatgtgataa ggatgaaggc cactgtcttt gaacaaccaa agcgggggaa aagcccggta
     3001 cggtggaccg tggggggtat cgccattggg ggtggggccg acggggagtg cgagcgcagc
     3061 gagcttgagc accgttaggt gcgagggggg caagcccccc tcccctgtcg ctgacaccca
     3121 tctctggtgc tctggatctg attgagagac tgttcccttc gccccccgcc ccgaggggcg
     3181 ggggcagtgc gtggcgatag ccaatggaaa gccgtgcact ggttcgtgca attgactgga
     3241 gaacggaaaa agaccagata caaagtgaca ggcaacgaca tcagaacggc aggagcaccc
     3301 tacctcgctg caccgaaaat cagaatgaag aatacggatt tacagcccgc aaaatggaag
     3361 aaacgccctg ccggagagaa gaatccggtt attcctttcc cttcagtgcc tgctgtgcct
     3421 tcttcgcccg ctctttcgcc gtatacccgg tcacctgctg gaggtagttc ctaaaccggc
     3481 ggttggtgtc gatgatcgtc tcgtcgtcgg catctttgtt cgcctcggta tcgacgatca
     3541 gcgtcttgcc ttcagccccg ggccagacct caaggcgcag gagcgctccg taggtgatgg
     3601 tgtagcgccc ttccggtgac gcacggggtt cggtcccgaa ctggtcgcgg agaccctgcg
     3661 ccatggaatc ggtaagagtt tttgtgcatc cgcgcttgat ctggtattcc tgcataatat
     3721 ttttaaggga cacccagcta tgttaaacta atccaggagt gtgtgattga tcccatggac
     3781 gatataaagg tcatatccag agcgctcgct ggcgccgaaa aaacggtgct gatcgggttc
     3841 cccgggagcg ggctcgtcgg gagtattgcc ctgcaatacc tcgtcgaaca gttggagttc
     3901 gaacagatcg gggcgatcac gagcaaatat ttcccgccgg tcgccctcat gacgaagggc
     3961 gtgatcaacg cccccgtgcg tctctacgag aaggatcacc tcgccgcagt catctccgat
     4021 gttcccattc acccgatgat ctgctacgag gtggcaaacg gcatcatgga ctggctcacc
     4081 cagttcgata tcagggagat cgtcacgatc gcagggatca tcacgaacga acccgagaaa
     4141 agggtctttg ggatcgcgac cagcagcgag acgctgcagc gtatcgagga ccagacgatc
     4201 attcttccca tggggagcat ctccggtgtc gcggcgagca tcctcacgga gtgcaagaca
     4261 cggggcatcc cggggatagg actcctcggg gagacggtga acacccccga cccgcggtcc
     4321 tcggcggcca cgattgaggt cttgaacaag atatacgacc tcaatctgga catcaacccg
     4381 ctgctcgaac aggcggtgga gatcgaggcg gcgatgtccc agatcgccga gcaggtgcag
     4441 aagaccgagg cctcgccccg gagagagcag ctgccgatgt acgggtgatg gctatgaaat
     4501 atgccgcatt aacaggaata tcgccgtcag tcattgccga actcaagacc ggcaaggccc
     4561 gcaccctcga actgaacagc gcccacaaca tcatcacgct gaccgagacc gccccggggg
     4621 agtgcatctt cgtaacctcg gtcaacgagg aagacctctc gcccggagac ccggggatca
     4681 tggtcgacct cctcgtcctc aacatcggca tgaaacggat cgaggtcgcc aacccgttct
     4741 tcttcgagga gcgggagcgg atgtcggccc ggatcaaggt ccagttccgg ggaacgacca
     4801 tcgcccgcga cgtggagggg cggaagtggg gagacccgac caaggtcgag atcatcaggt
     4861 cggcgtgtta ccgggccggc tgatacgctt tcttttttaa tagtcgggcg cccggcccct
     4921 caaagggtta atatcccgag acgaccaaaa tgtataggaa tgctacaggg gtctgtggct
     4981 tagccaggac atagcgccgg gcttctaacc cggacgtcgg gggttcgaat ccccccaggc
     5041 ccgtcgaatt ttgagatctg tttgccgtaa gaggaataag tccgggcagg atcgcggatc
     5101 gctttctgca atcagcttat cgacaaatca gaagatatta tgtgtcgatt gcgcgaggat
     5161 ggcgagggct ttatccgaga gggagatgtg ccgggttttt cggataggct cgttgtgggt
     5221 tgcgctcttt tatctgtttt tgctcagggt tttctctctg tagccacatg tggctacgtg
     5281 tgggcacact gtgaaagagg atccagtact tcgctgattt gagcactagt ggaccgattc
     5341 ccaataacca ttatactcaa ttgggggagt gtgatctggg ttcctgggcc acacccaggc
     5401 acagatttct caggactatc gccacgcacg gcccccgccc cccaggggcg ggggagaaaa
     5461 ccccgcacct cttggtgctc aagctcgctg cgctcgcact tcgcgtgagg tggcggtgga
     5521 ggcagtaccg aagtctcacg cgaaggacgc gaaaccgcga agagaggtta gttgtttgct
     5581 ctccggggga gactccctca actattttac cggactactg agtcaactgg agatccagaa
     5641 gatagccaaa gtgagggagg ccattctggt tcgatccgga actctacaag aagcgcagtg
     5701 cagtagggcg gttcttcaga tggattgagg catttaagaa gatcagtcta cgttacgagc
     5761 gatatgagca ctcattcatg ggattgattt acctggcatg cagtgtcatg atcgggagaa
     5821 tattgggatg acatctctag tgcatagatt ccaaataaac aacgtaataa atagcatgac
     5881 gccccacttg gagtatgccc tttgtctctg tccgtgagcc cgtactctta tctgatgagg
     5941 aacttgacta tttggagaaa ttacttcatt ctcgcacgac ccaaaagggc acgtacgagc
     6001 gtgcgaagat ggtcctcctc agccatcaag gcaaatccga ttctgctatc gcgcgtgaat
     6061 tagggctctc tcgtcagaga gtgatccgaa ccatccagcg cgtcctcgct gtcggtatcc
     6121 gggaggggtt gaacgatctc ccccggagtg gtcggccgaa aaccatctct cccgaagcca
     6181 ggacatggat cgtgagcttg ctatgccaga aaccttctga tttcggctat cctcatgaac
     6241 tctggacgca gcggctcttg cagcagtata ttcgagaaca cgccatggaa gaaggatttc
     6301 cggaagttgc ccggatttcg caagggacga tctcaaaaat tgccaatgcg agtgccataa
     6361 aacctcataa aatccggtca tatgtccaga aacgggatcc agcgtttcac gagacggcag
     6421 ccgtcgttct ccacacctac caggaagcga ataggttcaa gattctggaa aaagaaggca
     6481 aagagccgga tacatttatt ctctcgtttg atgaaaagag cggcatccag gtactcgata
     6541 cgaagtaccc cgatctgatg ccggtgcccg ggcaattctc ctccattcgc cgggattatg
     6601 agtatgttcg caacggtacc atctgtttgc aggcagcgat ggatctggtg accggatttg
     6661 ttcattaccg gattaccgaa cgcaatcgga gcgaggaatt catcgcgttc ctgaaaatgc
     6721 tcgataccca gtatccggac acgatgaaat tgaaaatcat cctggacaac ctcaaggttc
     6781 actcctcggt tgcaacccgg aagtacctca aaacggttcc gaatcgattc gaattcattt
     6841 ttaccccgaa acacgcgtcc tggctcaata ttattgaagc attcttcagt aaaaccactc
     6901 ggacggtact gcgaggcatt cgagcgaaat caaaggaaga gattaccgat cggatttcgc
     6961 agtatgttga tacgttgaat cagacaccag tagtattcaa gtggtcatac acaatggatg
     7021 caaatccctc cggggtggaa atggggtagt caggttaatt tagaatcgat gcactagtaa
     7081 ctacgatcgt tccaccggca accgccatcg aagttcgtgc accctcgata tagctcatta
     7141 ttcttttgag ctctcctcca tacaagacgg gaaccgcaca gagggcattt ccctgcatct
     7201 ccccctcttt catgcgacat agacttggaa gcatggtcct cgatgcctat aaactcattc
     7261 acccaagtat cgacgatgtt gctgcagagc cgccgtgctt tctccaccga ggcttcacta
     7321 tccccgagta tctccagtgc aacgcagccc tgccgtacca gatgagtcac tttgtcccca
     7381 gcctgaatac tctccagcgt tcccgccagg agctgcattc ggtcgaggat ctcgttgtac
     7441 ttctgcgatg tgcagcactg gtcgatctcc tgcaccagtt tgaacgaacg aagaagtttc
     7501 agggcagtgt cccggtcgta tgaacgccgg atgatattct ctgcatactg cccggcggcg
     7561 gcggggtttc ttgagagtgc gacgcgcagg gtcgcgacgt cgcacgagtg ggtgaaggtc
     7621 ttctcgcgga tcggaacctc gacgctgctg ataatgaact tcccctggat ggtatgcgat
     7681 ataccgcacg actccgagcc caacttgaag tacaccctgc gggaaccgtc tcgtgggatc
     7741 gcgatcccct ggtggcgggt gatcccgagg atcgacctta cctgccaggg ttttgggttg
     7801 ctggtcatga ggtgcatctg cccgtcgatt ccctcgatag cggtgtcgag gacccggagg
     7861 gacgacgaag agtccagttc gatcccccac tcgtcgatgg cggcaccgcc gtagttgtgc
     7921 acacagatga ccagccagta ggaggcgggg tcgtgctggt cgatccagtt cctgacgttg
     7981 acaagggggc ggggggagga gacgatgaag tccgtggtga gggggtcata gacggtgcgc
     8041 tcaatggtga tggattgtcg agtccccgcc ggctctccgg gatgtgcgga gggctgcagt
     8101 gactgcccgg cctcgcccga acaagggggc gtcgaccggt tttttcgagc acttttagcg
     8161 ctctcaacca atatacggtc tcgcatattt tgtgcggttg catctgtcgg gtcgagctca
     8221 agtgccctgt cgcaacatcg gatcgcctcg tcgtaccggc cgaggtgatg gagggcgtcg
     8281 cctttattta tccatgcaat agcaaatttc gggtagatct caagtggttt gtcgtagcac
     8341 cggatcgcct cctcgtaccg gtcgaggtta tggagtgcgc agcctttata gtaccatgca
     8401 tcatcatcgt acgggtcgat ctcaagtgcc ctgtcgtagc accggatcgc ctcgtcgtac
     8461 cggctgagga tcaggagtgc gcggccttta ttgctccatg caacagcatc tgtcgggtcg
     8521 atctcaagtg ccctatcgca acacaggatc gcctcatcga agcggcggag gcccaggagc
     8581 acggagcctt tattccccca taccgcagca cattttgggt cgatctcaag tgccctatcg
     8641 taacatcgga tcgccttatt gtccccaccg gggctcttct ggagtgcgaa gcctttgttg
     8701 cgccatacaa ctgcattttt cgggtcgatc tcaagtgccc tgtcgcagca ccggatcgcc
     8761 ttgtcgtacc ggccgaggtt caggagtgcg aggcctattt tctcccatgc aacagcatct
     8821 gtcgggtcaa gctgaagcgt cctatcgtaa catcggatcg cctcgtcgga gcggcggagg
     8881 gaatggagcg cgaggccttt atccccccat acagcagcat ttttcgggtc gatctcaagt
     8941 gccctgtcgt aacacaggat cgcctcgtcg taccggttga gggaatgaag cgcgtagcct
     9001 ttattgttcc aggcacccgc gttgttcggg tcgatctcga ttaccttttc gtaacactgt
     9061 atcgcctcct cgtactggtt gaggagatga agcgcattgc ctttattgtt ccaggcagcc
     9121 gcacagttca tgtcgatctc gagcgccctg tcgtagcaac gacactgttc ttccggcgtt
     9181 gctgcctgat tccccttctc aacccacccg ggagctgttt ccttcttatc ccttatccat
     9241 ccaaagattg ccattcactc cctcacgaaa tctccaatcc ggccaaagca ctcgcgcatc
     9301 tcacgaagta tgtgggcatt ttacctgctg cccgcaccgg gtacaaaatc tcgctccgtc
     9361 gcttaagggg ttaccgcatt cggcacagaa cctctgctcc tcgtcgtccc ctcccccgac
     9421 ggccgaccgg ttcacgacac tatcataaac ctgaataccc ccgctcgaag gcctgaactg
     9481 cccctccgac tggttgccga taccggcccg gttcatgata ctgtcgatga cctgcacgcc
     9541 gttcccgaga ttcaccgacc cgccagagat attgtagacg atctgttctg ccctggcact
     9601 cccctgattg atgtaggtga agaaccatcg caggccgttc ttctgcacga ggtctaccac
     9661 gacctcgtgc agtctccgat cgtacttctc ccactcatgc ttcaggtagt ggatgaggaa
     9721 cggattcgtg gcgtgcggac ggttgcagat ctcgttaatg aggctgtcga tatgctggta
     9781 gtgcacctga aggaactccg aaccgccccc gtccggcgcg ctcagcctga caatgagttc
     9841 gctcttggac ggaagaacct ggtaccgctc gatgatctcg tagagtgcga gctcgtccgc
     9901 ggagtacatc ccttcggcat ccgcgtagtt ctcgccactc cgacggaaac tcacggcgag
     9961 ggtcagcagc gccgcctcga acttggagca gaacacctgg tgaaacgtac tgtcaaagga
    10021 accacattcc cggttctcat accgggagta gttttcccgg atcgcacacc agagtctcac
    10081 ccgctcatcg taagactgcc tgacgatcgc cgatccactc ttcaggactg tctcggccgg
    10141 ggcaacgagg ttctttgcac aggtaaaatc ttgatctcca gacatacggc atcagaattg
    10201 agtttttgtg atggtgacta aaggtgaagg gcgagttgcc ggatgcacgt ggcacatccg
    10261 gcacagcctt ccacggctca cctgagttcc tcgccgcact ccttgcaaaa ccgatcggcg
    10321 gtactgtttg gcgttttgca tccggggcat atccggctct cttcctgccg ggcagggttg
    10381 atgatcaccg gctggccgag ccctccgccg ccggcaacga ccgtcgagcc ctgtgcctgg
    10441 tgggcggagc tccgtgcggt cgccgtctga cccatggcat cgagcgcctt gttcatgacg
    10501 tcggccaggc gatccgcgtt acctccggcc attgcctgga tcgtttccat gtatttctgc
    10561 tggtcctgca ggcgggtttc gaagagctcc tgcatcctgt cggatccgaa cttctctttc
    10621 atcgcctctg caacggcggt actcttttct gccgaaagcg ccatgatctg tccttcggtg
    10681 agggctgctg cgcgcttcat cttttcgagt tccgtaagct tttcagcggt accgccgtcc
    10741 gtcatcgaga taagcgcctc gggtgaggca ttgatacgct gttcgagttt cttcccctca
    10801 atctcggttt gcttctcgag gtcttcgtgg gctagctcct gttctccacg cactctctgg
    10861 agctttgcat cgtccatctg ctttttcagg ccgatgagat cctctatctc ggcatgatcc
    10921 tgctgtcgct cccgccctct ccacttgttc aggtgttcac gccgggcatc atcggtgccg
    10981 atctcgtgcc ctgtctgctc ggcatccatg gtacgtccgt gccgctgcgc ttttgcgtcc
    11041 aggccaagga gattgtcctg ctccgcttcg gcagactcgt gacgatgagc ctgctcaaac
    11101 cgctcccgga taatctggcg gatgaaggcc ttatcgtcgc gccggtttcg aagggcgatc
    11161 ttcaggtctt cgatctcgtt ctcgcgaacg atgccggcct gagccagctc gtgacagtat
    11221 tcgctgattt tctcgtcggt agccagctgg tcgagtttat cgtcggagag aaggtgccgc
    11281 agtttttgtt cgatatcgat ctgaagtccg gtcttcgtga cctttttcgt cccttcgctg
    11341 cgctgttcaa ggatatcgtg ccactctgcc tcatcggatt gcgtgaacct gagttgttcg
    11401 agggaaaagc cgtaactcga aagggtaggg gtgacgtttg ccctgacggc actctcgatg
    11461 tcttccttta cggcgaggtt cccatagaga tcgtccatcc ggtatccgct cagcgtggac
    11521 tgaacgacac caaggagatc gtatcggaga tcttcctgga gatccgcaag gaggattcgg
    11581 cccttccctt tgaggagatt tgcgatgaac gatttcgaat ccctgtttct gatcgtaaat
    11641 cttccggcaa tcccgacggg gacggagtcg gcggtgcgca gttccgagcg cccgatggtg
    11701 aaatcgattg caaccacgcc atcgtcgatg agcacgaccg tcgcgcgttc gtcgaggttc
    11761 aggggcagtt tggcttcgag aatatttcca agggtgtagt gacctgccgg caggtcgccc
    11821 gcatatgctc ctccctggaa aagaagcgcg cgggttccct cggcaacggt aacggtcttt
    11881 ttcaggacgc cccggagatc ttcaacctca aatttactcg caaaatcttc aggggttcgt
    11941 ctccattcgc gcctccgcga aaagtcctct tcgttagctc ctacgatcct tccgaagaga
    12001 gttttttcaa atcctttccc gcaaccgggg cagaattttg catcgggagg cacggtaacc
    12061 ccgcagctgc cgcaggtata cgcaccgacc ttcgttccgc actgcgggca aaactttgca
    12121 gcggccccaa gctggatttt acatttagga caggtaatat ccccattggt cattgctcat
    12181 cccttccctg cttaccggga aatttatcat actgtgttat agtcgttctg attctctcct
    12241 ttcccgatag agacccatcc aggagatttt ttacgattac ggcaactgga actgctccgc
    12301 cacgaatctc ttccattgcc tgccgtatcg aggtttcatc gacaggatgg tctttgccgc
    12361 ccggaatctg ttcggagaga atggcttgaa ggtcttcgac acggtttctg tcgccttcct
    12421 catgtacgga ttgctttgcc agccattctt cgagcttgtc aggagagatc ctgttgagtt
    12481 tctggcaggc gtccgacagg ttcagcatcc tgtcacgatc tctcatagcg atgatatacg
    12541 agatcaggcg gagatcgcgc tcaagtttca cgcaaactgc attcttcatg tggtattgtt
    12601 ctatgagtgt ccggatccgc attgcacgat gggcatcgcg cggctcgtcg catatcgagg
    12661 caccatcgtc gagcaggttc tgtatctttt ctttgaggtc ctgagattcg gattcgaggg
    12721 tgccgatccg gcacttcagc agcgcttcgg ccttgcgtag atcgctgatg ctggacgagg
    12781 cgaacgggtc tttcggatgc ctacgggata gaatcttcat ggtagtgacc acccatcacg
    12841 atctgagggg ttacccttcg gattcgcgga tcgccttctc gatatctttc tcaatcgaaa
    12901 agagatcttt cgcttcatca agccctagcg aaccggtctc gacactgctc cagacttcaa
    12961 acatctgctt ttcagcttcg tcgaggtcct gatccgagtc cgaaagatca agggcaaaca
    13021 cctgatttaa gtcgtcgacg acagtttcaa aattctttcc ggagagattc acgcggatga
    13081 gggcactctc gatagtttcc ggagagatcg tctggatctt ctcccagatg tgtgtcttct
    13141 ggagatcccg ctgatacttt ttgatgataa ccaggttcga gataaacatg tactgtttat
    13201 gcagggcaag gaagtttcga accttgagct ttgcagtcat atccagttgc ttgagttcct
    13261 gagcaagcat cttcttcttg ataaggtcgc cgccgacgcc ttctgcaaat ttcagttttt
    13321 tctcgcgctc gattctgtcg atctccttcc ggaggcggtt gatcctgctc tttaatatga
    13381 tctcttcctc ctgcagttcg gtgatcgtca acatctctat ccggttccga tgaaacaggc
    13441 ttgcgatctt catcacactt caccgattga ccattgccct ccatacatga taaactttcc
    13501 tccaatatgg aggatattaa tatactccaa aatcaataac tccactgcgg aggaatatat
    13561 atagtggtac ccgcatatgg ttatcaaatg gaatacctcc tcgttgtcga ctaccactca
    13621 gatgccgagc gtaagcggat tgacgccacg attgacagat ggaagtcgaa aaaagatgta
    13681 cacaaagaga ggggcgtcgc cattctggtg agagacgagg tgattgaaga cttcctgcag
    13741 gacctctacg cacggctgga acggggaagt gaagatgtca gaatatattc tgcagaggaa
    13801 tccgtgcccg atattcgcga agcgacaaag actctgtatt atgagacatc tctggataag
    13861 gacgcattaa aacaattttt aagatatgtc ctgaataaac tcaattcaag ttatgagggg
    13921 gtccgcaacg gagtcgaatc gtatactgcc tatacccgaa agggacaggc gaccgtcggg
    13981 cttctctggt ccggggatgc aggggcaacg catctcaacg taacggtcag cgggtttggt
    14041 gatgtggtct cgcttgtggc cggccggata gatgaggaac tccaggtctt tctaggaagt
    14101 ggaaaatgaa tcagacagac aaccatgcaa agtacatgtt taatctgagc aaggggcagg
    14161 aactttacaa agttcagcgt tttgaggatg cgctcaagta ctttaacaca gccctggaac
    14221 tcgaaccaga ctctcattgg gcgtggaatg aaaagggcaa tgcactcagg agacttggaa
    14281 ggcaaaggga agcgagggat tgttacgagc gggcaattca cctagacaaa aggcactcgt
    14341 atccgtttcc atacatcggc gtcgccgaca tctaccgcga tcagaagcag tactcagagg
    14401 caattgctta ttatgacaga gcaatacgga tcaagagaca cccatgggca ttgaacggga
    14461 aaggaagatg cctccacgct cttaatcaaa ccgatgaagc gctcagactg gctgaagagt
    14521 cgatacatat tcgccctgaa tttctctttc cctacattct cagaggtgac atcctctatg
    14581 agagggctgc gtaccgcgac gccttgagat catatcaaag ggcatacgaa ctgatccgcg
    14641 gcccctcgcc tgaacttcgg ggagaagtga agagcaaaat cactcagtgt actgcgatgc
    14701 tcgatcgaga tgttcgcgtg gagccgggta atcaagaaaa gcctttgaat gctctcatca
    14761 gagcgttcag cgaccacatc cccagcattc gaaaaaaact acatgatgca cctgcggcca
    14821 caagcatact ggatgagaag aacggaggct attctcatcc ggaatcaaat tcgacagaca
    14881 atcatgcaaa gtacatggct aatctgaaca agggaaagga gttttacaaa gttcagcgct
    14941 ttgaagatgc gctaaagcac tttaccacgg ctctggaact tgaacccggc tcccactggg
    15001 cgtggaatga aaagggaaat gcactcagaa ggcttggtcg gcaaaaggag gctatagagt
    15061 gctacgagcg ggcaatctct cttgacagag cgcgatcata tccgttcccg tacatcggca
    15121 ttgccgatat ctaccgcgag cagaagcagt attcagaggc aattgcctac tacgacaggg
    15181 caatgcggat caagagacac ccgtgggcat tgaacgggaa agggatgtgc ctccacgctc
    15241 gtaatcaaac cgatgaagca cttaggctgg ctgaggaatc gatacgcacc cgtcctggct
    15301 ttctctttcc ctacattctc agaggtgaca tcctctatga gagggctgcg taccgcgacg
    15361 ccttgagatc gtatcaaaat gcattcgacc atatccacag ttcttcgaca aaacttcggg
    15421 aagaggtgag gagtaaaatc gctcaatgta ccgcgatgct cgatcgagaa cttcgcagag
    15481 aatctggcaa cgatggcaat tcggatgtta aacggctgct tatacggctc gggtcggaag
    15541 atagccagga tcggattcat gcatcgtacc aactcgacat cctggccaaa ggaggaaaag
    15601 ccggagcgat cattcgagaa agagcgtttg atatgctcgg cagagcgctc gacgattccg
    15661 tccccgatgt tcgaagaaac atcctctggg ttctcggaaa ccttgcttac cagggttatt
    15721 cgtatgaggt tgccggatca gggatccttc ctgaaatggc cgcacatctt gacgacccgg
    15781 cagacccggt gtgcagcgcc gcggcatggt ctctggcagt ggttgccgaa gcgggtcagg
    15841 gcattgcggt cgcagattcc ggcgcgactc tgcgctatat ccgtcttctc tatagcaaaa
    15901 accacgaagt ccaggcatgc gctgccctcg ccctcgacaa gatcgctttt tatgcaagcc
    15961 ccgaaccggt ggtgcgaaat ggcggtgcta ctgcacttgc ccaccttttg aacgtacaaa
    16021 atgatgaagt tcggtttcgt gttttatggg cgctctggtc aatctcatgc agggggtacg
    16081 cggattcgct atgcgatgcc gaaactctcg tcacggatct gaaacgatgt gcacaggaga
    16141 aggacctgga gatccgaaaa gcggcggtat cgatcatcgg tgaactttca aaggtacgcg
    16201 ataagtcgtt tttacagcag aaagatcttg aaaaagtcct cctcacgtgt attggttcaa
    16261 ggagcaaccg ggtcaggggt gcggcaatct gggcagcagg gctgtgggcg gacgcaggat
    16321 gcgctcccat gctgatccag gccggtgtga aagaagcgat tagaaacaac ctggatgacc
    16381 gcaccagagt tcacgtcttc aaccatagcg aacaccggtg gatagagcgc tctatcggcg
    16441 ggattgcagg tgatgtcctg aagaaaatcg tagcgtctcc ggacccggtg ccgcccaaac
    16501 cggatacccc ggaggaaatc gtagttctag cggatcctgg gtcgcccgaa ccggactacc
    16561 ggacggcata tttcgttgca caaaaccttg tggaacatct gcggcaacgt gatcgtctgg
    16621 aagcacgtaa atctgcaatt cacctcgaag tgttcgtccg tggagatatc ctcgaaaaac
    16681 tgcaacaagt aagtgagcac ctgcgttttt acatcgacga ggacctcgac aatgagattg
    16741 aatctcttgc cgatgatgtc gaattactca aggagaacat ccttcgagaa gcgatgaggt
    16801 gatgactgtg cacgtggacg ttagcggatt tttgcttgcg gaactgcgga aaactattac
    16861 tggctataga aaggcagtag ctgaagcgga caacgcgact gctaacgaga gggcgcgggc
    16921 gtgtgcaaca ctctgtcggc agattgcagt ctcttcgccc atgatgcagc aaaggtatac
    16981 cgccgaggca gagagatgga agggtattgc atcccgttca gcgttggttg cacagagaag
    17041 acaaagcaag acatcgggcg cacctgggca ttcgaatcgt tctgttaaag agcttgcagg
    17101 cgatactgag atgtcgtcgc tgatcgcgcg ctcatcggtt cgatgggagg atatcggcgg
    17161 gcttgacgag gtcaagcgcc tcgtgaagga gaccgttgct atcgcagccc tgaaaagacc
    17221 ggaatctatc cgtccgtgga agggtattct ccttttaggt ccgccgggta cggggaaaac
    17281 aatgattgca gctgctgcgg cagggagcct ggatgcaaca tttttcaacg taaaaaccga
    17341 ccgattgctc tcgaaatact ttggcgagtc ttcgaagatg atctcttccc tgtatgccgc
    17401 ggcgcgagaa caggcccctt cgatcgtctt tatcgatgag tttgatgccc ttggtgcatc
    17461 gcggagcgac acgatgagcg aagcgacccg gagagtgctc tcctctctcc tcatcgagct
    17521 tgacggcctg cagggcaaaa aagacgaccg tctgctcctc acgatggcgg caacaaatgc
    17581 accctgggat ctcgattcgg caatattgtc gaggttcccg cggcgaatct atgtgccgct
    17641 cccggatatc gatgcctgca gggcaattat cgagattcac atgcgagggc ttgaagtatc
    17701 cggagttgac ctcgtcgaac tcggacggca atgcgtcgac cgcctgtact cgggacgtga
    17761 catccagaat gtctgtcaac aggcgatctg gacgatgatc aacgatgtca atcctgaaat
    17821 tcaccggctc gcggatctgc cgtttgatga actcaaagag cgcgcgctga taacgcgacc
    17881 tctctgtgaa aatgactttg cagccgcatt tggcaagata cgatccccgt taaaggtgga
    17941 agatcttgag cgatacgtgg aatggaatgc aaaacatggt gatgggtgag ttcgggtaac
    18001 tagtgcatcg attctaaatt aacctgacta ccccatttcc accccggagg gatttgcatc
    18061 cattgtgtat gaccacttga atactactgg tgtctgattc aacgtatcaa catactgcga
    18121 aatccgatcg gtaatttcac tttgcgaacc cctttc
//
LOCUS       DAJB01000002            2542 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_185066, whole genome shotgun sequence.
ACCESSION   DAJB01000002 DAJB01000000
VERSION     DAJB01000002.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_185066"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    413..567
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          418 a          758 c          759 g          452 t
ORIGIN      
        1 ggcgcagcac cccgggagaa gtctcccccc tcgatcgcat cggtcacctc gacatcgaac
       61 gcatcgctcc cggccccgga gccaaacgat gcaacgaata tccggtcccc ggctttcgcc
      121 acgtcgagcg ttgccgcgag cccgatcatc gaggagcccg agtaggtgtt cccgagccgt
      181 ggcaccacga ggcccggccg gatctggtcc tccgtgaacc cgaggatctt tgccacccgc
      241 tgcgggaact tggcgttcgg ctggtggaag accgcgtagt cgtagtcttt cgggctcctc
      301 cccgactggt cgaagaggag cgagacggcc ccctcgatgt gccggaagta cccgggctcc
      361 ccggtgaacc gcccgccgtg gcggggatag tcctgcccct cccgccgcca gannnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnntcg gcagccgtca tgcagggcgt ggccccgatc
      601 gcctccccga ccgtgcaggc cgtcggcttg acagcatacg gatgggactc ggagcccacg
      661 tacaccgccc cgatctcgcc gggatcgata tccctcctcc ggagtgcgtt cctcgccgcc
      721 tcgaccgcga tcgtggcggc gtcctcgtcg atgtccggga ccgacttgga gaagacgcca
      781 agcccgccga tgatgtcatc ggcgtttgcg ccccagacct tcgtgatctc ctcgactttt
      841 atccggtagc gggggatgta gaccccgtac gtgatgattc ctaccatcgt tcttccctca
      901 ggagttccat gatcctgtcg accggggtgg tcgtggtgag caccgtgatg tggtcgcgct
      961 cggccatctt gggcacgagc gggtggacga gctcgggctc gatcccctgc agcaccacgc
     1021 agcgcggctt gaacggggtg acccggatcg ccaccatcgg ggacttgccg gaggtcacat
     1081 ccgtgaagat cagcgcccgt tccgtgctcc acccgtagat ccggttgaac tcgttcgagg
     1141 agagctgggt gatcgcgttc aggctgttga cgacggtgta cccgaagatc gactggctca
     1201 gcgagccgca cagggtcttt gcaccgagca gttcagagaa ccgggagagc aggaccgggg
     1261 acgggtaatc cacgatgtcg tagatgacac tctcgtcgat gtcggagtac aggatcttgc
     1321 cgaatttccg tatgtacttt cccccgttcg cctcgtcgat tgcaaggatc gtctccacga
     1381 tctttcccac gaccgcggtc ccggggctct tcctcctccc gctctcgtaa tcactgataa
     1441 ccgaagggga gacctcgagg tgctcgcaga gcacgccctg gggaatgttg aagctcgtcc
     1501 gccacttctt cagggctttt cccggcgact ctgaaagcgt gatctcgccc gccatcttct
     1561 cggcaagctg atgacgcaga tcagatttca tggctcacta tgaggggtgg agggatgata
     1621 taattaccga atacggcttc gacatttccc gaatgaccgt ctgcgggatg cctgcggaga
     1681 tactcataaa tagtaagcat ctcaattttg atacatatga tcgtgtccgg ggatctccag
     1741 tgcctgaaag agatcgccct gctgggtggc ctgaagggcc ctgtcttcgt ctcatcccag
     1801 tccctggcgg gcgcgctcca ggtcagccag cagaccgcat cgcggaggct ccaggccctc
     1861 gagcgccagc gcttcctctc gaggtcggtg aacccggacg gccagtacat cacgatcacg
     1921 aaggggggcg aagaggcgct ccggcaggag tacgcgtcct actgccgggt tttcgggacc
     1981 gaccggcagg agttcctgct ccaggggtcc gtgatctccg gcctcgggga gggccggtac
     2041 tacatgagcc tgcggcccta cgtggagcag tttgcagaga agatcgggtt tgagccctac
     2101 cccgggaccc tgaacctccg gctcgaggcg gcaagcgtcg gggtgcggaa gcggctggac
     2161 cagcttgcgt gggtgccgat ccgggggttc atggcagagg ggcgcacctt cggcgacgcg
     2221 cgatgcctgc cctgccggat cgccgcgatc ccctgcggga tcgtggtccc gggcaggtcc
     2281 cactacccgg acgatgtcat cgagctgatc gcgccagtct ccctccgcga ggcgctcggg
     2341 gtaacagaca acgatggagt gaccgttgag gtggcaccat gattgagaag gcactggaag
     2401 cactgaagaa cgggcaggta ctcctgctct atgattttga tgaccgggag ggcgagacgg
     2461 attttgcgat acgatcggac cgggtaacgc cccgggacat caccctgatg cgaaatgacg
     2521 gcggcgggct catctgcacg gc
//
LOCUS       DAJB01000003            8247 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_22963, whole genome shotgun sequence.
ACCESSION   DAJB01000003 DAJB01000000
VERSION     DAJB01000003.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_22963"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1325 a         2694 c         2626 g         1602 t
ORIGIN      
        1 gtttcaaccg ggggtagttg accaaatcca ccgtagttct aaagtgtgat aagagaaatg
       61 attgcaactc atataaccgt ttctaaccgt cactacgaag acggatgtcg gaacgaatgc
      121 tgattttgca gttcccgtcc ctgaagatgc gcaccatgcc cccgctctcg gagaccgtga
      181 tgccgatggc cgggatctcg tgcgtaatag acgcggttgc gcggtgcctc cctccaaggc
      241 ccccgggcag agagatgccc cgaccggtga cgtccaggta gcgccccgcc gaccgtacct
      301 cgccgctctt gtcgatgacg aagacgccgt cgagctgggc gaactctttg acgctctccc
      361 agttatccct gttcttgatg tcccggaggg cggcgggctg cccctggtag gggttgagga
      421 tcgcctggtg ggagtgcttg aagatctctt ccggatctcc tatgataaat gcggtgccga
      481 tggccctccc ttccctccct tcgaccgcga tctcgagggc cagcgtgagg gctgcatgga
      541 gcacctcacg ggggacgaca tcggagaagt cttttaggtt gatgaagttc ttcccctctt
      601 cgatgtcgta gatgacgacg gcgtagggaa agacaccgac gacgagaccg ctctggaggt
      661 ttcgccggag atagaggtgg actgcggcgt cgagcatatg ccgctcgctc acctccagga
      721 tatcgtgcat ggtgaggtct tttaagacat cgagctggag ttcctgcacc ctgattatcg
      781 ggacctcggg cgtccccgga gggagtggaa ccggatcgac gaacgagatg actgccagtg
      841 cgccgatctt cgccgccacc tcgcaggcgg ttacaagcat caggtcgccg ttcatagcac
      901 ctcccggatc agtcccggta tctctgaggg ccgggatgca accgggatgt tgagggcgga
      961 gagccgtcga accttggacc gtgcgtcacc ctcgccgcct tcgatgatcg cccctgcgtg
     1021 gcccatccgc ttttcgggcg gggcgctcac gccggcgatg taggtgacga ccgggaggct
     1081 ggtcgaccgg accccttcct cttcgaggtt accgcccact tcgccgatga cgacgacggc
     1141 tttcgtatgg gggtcctcct cgaaccgggc gagcgcatcc acgaacgtct ggccgatgac
     1201 cgggtcgccg ccgatcccga tcaccgtgct ctggccgatg ccggcccggg tgagctcgtc
     1261 gaccacctcg taggtgaggg tgccgctccg ggagacgacg ccgacctctc cccgggtggt
     1321 gagatgggcc ggcatgatcc cgagtttgca ctcccccggc gagaggagcc ccgggcagtt
     1381 cgggccgatg accgtgcagt cgtggagttt tgcgtaggcg atcgccttca tcgcgtcgtg
     1441 gaccgggata tgctcggtga tgacgaccac aaactcgatc cctgcgtgcg ccgcctccat
     1501 gaccgagtcg cctgcggcgg cggccgggac gaagacgacg cttgcggtcg cgtcgtgttc
     1561 ccggagcgcc tcccgcaccg tgttgtagac cggtacgccg tggacctccc ggcccccctt
     1621 gccgggcgca acccccgcga cgacgccccg tccaccgacc tcgcgtgcgt aggcgttcat
     1681 cagttccgtg tggaacgtgc cctgtctgcc ggtgatgttc tgcacgatca ccgaaaggtt
     1741 tcgatcaccg tagatcatgg tacgacctcc atcgccgccc ggacggccag gtccatgcta
     1801 tcgagcatcc ggtagccgca tccggcgagc agccgccttc cttcctcctc gttggtcccg
     1861 gccatccgga cgatgaccgt cggtgcgacg gccgcggcga tgatcccctt tgccacctcg
     1921 tcgcaccggg tgataccccc gaggaggttg accacgatca cattcacccc gggcatgctc
     1981 gcgacgagcc ggacggcgtg catgacacgc tcctggtcgg cgccgccccc tacgtcgagg
     2041 aagtttgccg ccctgccctg atagtactcg atgaggtcga gcgtcgacat cgtgagcccg
     2101 gcgccgttgc cgatgacgcc gatggttccg tcaagttcca cgtaggagaa gccgtgcttc
     2161 tcggcctcgc gctcgcgctc cgagaggtcg cggttgaccg ctatcccctg gcgggcgagg
     2221 gcattgtcgt cgacgatcag cttcgcgtcc gccgcataga ccccctgcgg cgtcgtcacg
     2281 agcgggttta cctccgcgag catggcgtct ttctcctgga atacatggta gaggctgttg
     2341 atgacggggg cgagttcctt tggggcccca ccgaggagtt cccgcatgag aaacggcgga
     2401 atatcccgga gaagtgggga tacgacgacc ctgcggatag cagaaccatc cgctctcgcc
     2461 gcattctcga tctccacgcc gccggcgtcg gcaaagagga ccaccggctg tctgctcgac
     2521 cggtcgatgg cgatgctgac gtagtactcg tgctcgatcg ccaggcgctc ctcgacgagg
     2581 atctccctga ccgggacgcc cttgatctcc cgggagaaga gttcccgggc ggtctcttcc
     2641 gccgtcgcat cgtcggccat caggacgccg ccggcctttc cgcgtccgcc gacgtccacc
     2701 tgcgccttca ggacaacact gtcgccgatc tccggcagat gtgccgtaat ctcttccggt
     2761 gcccgtataa gaaccccttt cgggaccgga atgccgtagc ccgaaaatat ccgttttgcc
     2821 tcgtattcca gtagcttcat aggttacacg ctcgttctcc gagttcgaat ccccgcttta
     2881 acgccttgat gtttaagttc tcggtgcctt tggggacgct gtcgagcacc gcacgctcga
     2941 tcgcctccct gctcacgacc ccggttgcgg tcgcgagggc gccgatcatc acgatattcg
     3001 ccacgatctc ccggccgagg gggccttttg cctccgccgt cgccggaacc tcgcagtagc
     3061 ggcagatcgg gcgtgaccgg accaggtcgg cgtcgaggag catgaccgcg tcctcccggg
     3121 ccgaaacacc gtacttctca aacccctgct gggacatgat gacgtagatg tcggggtcgg
     3181 tcacctccgg gtagaggatc tgcgcatcgt cgatcaccac ctgccccatc gaggccccgc
     3241 cccgggcctc cgggccgtag acctgcgtct ggacggcgta cttgccgtcg tagagcgccg
     3301 ccgcccgccc gaggatgacc gcggagagga tgatcccctg cccgccgtat cccgagaacc
     3361 tgacttcgtg cctcatcgtt tcactcccat tgccggccgg ctccttcgga ccagttcccc
     3421 gacggtgaag gtgtcgtccg ggatcggctt tccttcctcg accagccggt cgcgcttcgc
     3481 gacaagcatc gcgtggctcc ggaggtgctc gatcatatcc gagacgcgcc ggagtttgtt
     3541 gtggcggccg tagttcgtcg ggcactgggt cctcacttcg atgaaggcga gccccggcgt
     3601 ctgcattccg gtgacgacgg ccttcgtgag ttccttgacg tggtaggacg tccagcgggc
     3661 aacgtagttt gcgcccgccg ccacggcgag ttcggcgagg tcgaaggccg gttcgctcat
     3721 cccgtaaggt gtcgtcgtcg agaaggcccc gagcggcgtt gtcgggcttc cctgcccgcc
     3781 ggtcatgccg tagatctggt tgttcatgca gaccacggtc atatctacgt tcctgcggca
     3841 ggcgtggatg aagtggttcc cgccgatggc ggcaaggtcc ccgtctcccg tgaagacgac
     3901 gacatttaag tccggttttg ccatcttcac cccggtggcg aacgcgagcg cccgcccgtg
     3961 ggtggtatgg agcgagtcgg tgaggatgta gccgggagca cgggatgagc acccgatacc
     4021 ggagacgaag gccgtctcgt tccgtttcca gcccatctcc tccaccgctg caagggtgca
     4081 gttgatgacc gtcccgttgc cgcacccggt gcagtagatg tggggcagac ggtcttcccg
     4141 gaaccagtcg ggcgcgatca tcggtgcgcc tccagggccc gcacaagttc ggcgggggtg
     4201 tggagttctc ccccgatctt cgggattgag atgaccggct ggtccacgtg gcgctggacc
     4261 tcccgcacca tctggcccat gttcagttca ggcatcagga agactttggc gtttgggaat
     4321 tcctgcaggg cgaactccgg gaacggccag actacccgaa ggcggaggtg gccgatcgat
     4381 tcgtcgggcc gatcgcgcat cacctgctcg accgtccggg acggcgggcc gtaggtgaca
     4441 aagaccgtct cggcgtcggg gttggtgatc tcgtagtcgg cgatctcgcg gcgtgccgac
     4501 tcgaccttgg cgaccagccg ccgcacgagt ttatcatgcg cctccgggtc ggtcgtgtcc
     4561 gggtagcccc gttcgtcgtg ggtgaggccg gtcacgtgga cggacctccc cgagccgaag
     4621 ggtgcaaacc cgggaacgcc gtcgtcgccg gcctcgaacg ggagcgagcc tcccgcgagc
     4681 gggccggggc gcgtgacctc gaccgcgtcg gggatggtcg cccgctcccg catgtgaccg
     4741 acgatctcgt cggccatcag gaaggtgggg accctgaacc ggtccgcgag gtcgaacgcc
     4801 ttcaccgtca ggtcgaacat ctcctggacg gagtttgggg tgattgcgat gatgctgtag
     4861 tcgccgtgcg acccgaaccg gcactggagc atatccccct gcgccgcccg cgtcggctgg
     4921 ccggtgctcg ggccgccccg ctggatgttg acgatgacgc acggcgtctc ggtcatggcc
     4981 gcgtagccga tgttctccat catcagcgag aacccggggc cgcttgtcgc ggtcatggcc
     5041 cggactccgg tccaggcggc gccgatcacc gcggcgatgc ttgcaagttc gtcctccatg
     5101 gagatgaaga ccccgccggc cttcgggagc ctccgggcca tcgtctcggc gatctcggtc
     5161 gacggcgtga tcgggtagcc gccgaagaac cggcacccgg cggcgagcgc gccctccgca
     5221 caggcgacat tcccctgcat gaactcaact ctgctcaaaa ctccacctcc acccgatgag
     5281 gctcaaacgg tttctcgtcg atccaggcta tcgcctggtc ggggcagatc atctggcaga
     5341 ccccgcagag catccgcccg tagagggcct gcagcctgca gttcgtacac cgttcggggc
     5401 ggtcgagaac cggcgagacg atcccccggc tgttgagttc cgtcccttcc ccaaagatcc
     5461 cgtatggaca gaccagaacg cagaggttgc accctttgca gcggctttca tcgatgacaa
     5521 gtttcatgaa cggtatcctg cttgatgttg aatcgctggt gaaattgtgt tttcgctttc
     5581 tcgcgtactg cctcgaagag atcgccgccg tgggcgtcga tcccgaccgt cagcggcagg
     5641 cgatcggcct ttatgaccca gaccgcctcg gccatgccga ggtcctcaaa atagacgcct
     5701 gcaaggctca tccggtcggc ggcgagtgcg gcgcacccac ccgtgagcgc gaggtagacg
     5761 gcgcgccccc ggagttgctc gaccacctcg gcccccatcc cccctttgcc gatgagggcg
     5821 cggacacccg cgtcgatgag gaatcccgtg agcgcgttca tccgggcgga cgtggtgggg
     5881 ccggcaacga cgagccggcc gttctgcacc accgggccgc agtgatagac cacggcgccc
     5941 tccgggtcga aggggatgcc ctccgccatc atccgcaggt gggcctcgtc ccgggcggtg
     6001 tagatcgtcc cggagagggt tagttggtcg cccgcacgga gcgagaggac ctcgtcgccg
     6061 agcggtgtcg tcaggttcaa cgcttcacct ccacggtcgc cctgcggcag gcccagcact
     6121 gcacgttcac cgccaccggg agggatgcgg tgtggcagtc cgcccgcttc accttcacgc
     6181 tgagcgccgt cgtatcgccg cccaggccca tcggcccgat gccgagggcg ttgaccgcgt
     6241 cgcagatctc ctgctcgtag tcgtccatgg tatcgaccgg gaggagcagg gcttccttcg
     6301 caagcgcagc cgccatatcg aacgtcccgc cgatcccgac accgaggatc acgggcgggc
     6361 agggtttgcc ccccgcaagg agcatcgtct cggcgacgaa cctggggatc tgtgccgcct
     6421 gtgaggggag gagcatcccg atcctggaga cgttctcgga gcctgcacct ttcgggagga
     6481 ccgttaccgt gagccggtcc cctggcgtca cgtggaccgc aggcatcccg acaccggtgt
     6541 tgtcgttggt gttctcccgg gtgagcgggt cgacgacgtt cggacggagc gggatcgtgg
     6601 ccgtcgcccg gcgcacccct tcccgcaccc cgctaaagag gtcgggcgag agcccgatat
     6661 cgggaggcag ggtaaggtag atcaccggca cgccggtatc ctggcagatc ggcacctgcc
     6721 gctccccggc gagtgcgata ttctccagaa tatggccgag ttcctgtcgt gcgatctcgt
     6781 ccctctcggc cgccgccgcc ctccggagca ccgcgagcac gtcgggaggg agccggatct
     6841 cggcctctgc aagagccctc tctgtggcgt ctgccagcgc gcccgaaagt gtcgtatctg
     6901 ccgggttaac catcatttat actggtggga agagactata aatatctata ttcccacggg
     6961 tatcggatct gctgtttgcg ggtcgttcct cgaaaaaagg atgggacggg gtgcgagttt
     7021 acgctaccgg taccagttct tcgataatcc tcgcgggggt cgggatcttg tagatgccgc
     7081 gccggaggga aatctttgcc ttctcgaggt actgcgggct tgtccagacg gagaagacct
     7141 tctgaccccg ttcaacccgt gccgcggtgc cgaccgcctt gccaaaggca agacgcatgc
     7201 cttccgagac acggtcggct cctgcaccgg ttgcctgctt gttctcacgg agaacgtggt
     7261 gcgggtaggt ccggatcttt aagtggtagt ttgccctccc tacttccttc tggagctgcc
     7321 ggttgatacc gatacgggcg gcttcaaggg ccgtgtggcg tatctggcag gcctcctcgg
     7381 caacgatgga gagctcgagc gggaagtcct ggctgaggtt gcccatatca aactgcacga
     7441 tcttgctgcc cggcacacca cccatgtatt ctctgcgtgt atatgctttc tttgcgagat
     7501 tcctgtacat cttcgctggt tttcgtacca tggttaaaaa ctcctgccga tctgatgagt
     7561 cctttataaa aaggagattt gctctaataa atcttactgg gcgctttcgc tctcgtcgat
     7621 catccggagc acctttcggc ggacttctgc gaactcggcg ctggtccggt cccgcggtcg
     7681 ggaccagggg atggcgatga tctcctggac cgtccctggc cttggtgcga ggacgatgat
     7741 ccggtcggag aggtagaccg cctcgtcgac gctgtgggtg acgaagacga tcgtcttctt
     7801 tgtctgctcc cagaggaaga ggagctcttt ctgcatccgg ttccgggtct gggcgtcgag
     7861 cgccccgaac ggctcgtcca tgaggaggac gtcggggtcg tttgcgagcg ctcttgcgat
     7921 cgccacccgc tgccgcatcc cgccggagag ctcgaagggg taggcgtccc ggaacgggga
     7981 gaggccgacc atatccaggt agcggtccgc ggtcttccgg cgctcctcct tgctgacgcc
     8041 tttcatctcg aggccgaagg cgacgttgtc gatcaccctc cgccagggga agagggagta
     8101 ctcctgaaag accatccccc gcttcgggtc cgggccggtg accgcgcggc cgtcgacggt
     8161 cacgcttccg gtggtcgcgg tctcaagccc ggcgatgatc cgcagaagcg tcgtcttccc
     8221 gcaccccgac ggcccgacca ggcagat
//
LOCUS       DAJB01000004            3092 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_210414, whole genome shotgun sequence.
ACCESSION   DAJB01000004 DAJB01000000
VERSION     DAJB01000004.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_210414"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    774..850
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2458..2468
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          435 a         1030 c          981 g          558 t
ORIGIN      
        1 agccgtggca ggggcagtcc caggtctctt cggcgctgtt ccaggcggcg acgcatccca
       61 tgtgggtgca ggttgggttg actgcgtgga ccctgccttc ccggtcgcgg aagacgccgg
      121 ctttctggcc ctcgatcatc aggaccctcc cctccccggg cggaacgtcc gcgaggtctc
      181 cggccggccg ggatatcacg ccgccgatgt acttctcggc cacctcgatg ttgtggacca
      241 ggaaccgccg ggccgatgcc gccggcttga accggtcggg ggcgtagacc tccgcccagg
      301 ggctcgcgcg gccccggatc atatcggtga ggatcgtcgc ggccgcggtg ccgttggtca
      361 tcccccactt ccggaatcct gtcgcgatgt agatgttctc gtggccttcc gcgagcgggc
      421 cgatgtaggg gacgccgtcg ttggtgatgt agtcctgcgc cgaccagcgg tagtcgacgg
      481 accggaccgg gtagatcgac cgggcatacg cctcgaggtc gcggtagtgc tccctcgtat
      541 ccacgctctc gccggcccgg tgctccatgc cggtgacgag taccagttcg ccgtcgccgg
      601 tgggctgtga ccgccaggag tgggtgggcc cttcggcgtt gatgaaggtc ccgtccggga
      661 acggctcgtc gatccggagg ccgaggacgt aggagcgcga cggctccata cgggcgtagt
      721 agaacccggg gctgtcgtag aagggatagt gggttgcaag gaccccggcc cggnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn accgccccgt aggtcctgcc cggcagcggc acctcctcta caaatgtcgc
      901 cgggagcccg aggctccgtg ctgcgtccgc ttctgccacg acaaggttgc gggactcctc
      961 cgactccgcg taggtgtagg cgggtttccg ctcgaaactg cacgggatgt cgtactcccg
     1021 gacaaccgat gcgaccgtct cgatcgccgc ctggttcgct tcggcatact gctgcgcctg
     1081 cctgctcccg aaccggtcga tgagctccgc gtagatgagc cggtggagcg aggtcacctt
     1141 cgccgtcgtg tggccggtca ccccccggca gatcctgccc gcctcgatca cggcgaccgc
     1201 gtatccggcc tgcttgagca gaaatgccgt ggtgatgccg gtgatgccgc cgcccacgac
     1261 caccacatcg gtctccaggt tccccggcag gggggatacg acgtctccgc cgttgtctcc
     1321 atccagaacg actcgtgcct gcccggcagg ccataagcac ccggtccatg ctccatgcgt
     1381 caagcctcct tctgcctgcc tgcccctcgt ccccgaacgg gtgccgggga aggggcggcg
     1441 gcggtcggtc ccattatgcc ggcaccatat ataggtatgg agcccgcctt cccccgcgcc
     1501 tacttccgca cgaccttctt ccctgcaagg tccttcaccg tggggctctc gatcaccttc
     1561 ccgtcggtat cgtagcggga gccgtggcag gggcagtccc aggtatcctc ggcgttgttc
     1621 cacgcgacgg tgcagcccat gtgcatgcag gtcgcatcaa gcgtggtgac tctgccgtgt
     1681 tcgtcccggt agactgcaac cttctgcccg tcgacctcga cgaccttccc ctcccggggc
     1741 ggtatcgccg cgacttcccg gtcgaaccgg gtcgtgtcga ccctgatcgt ccccccggcc
     1801 gcctcgagtt tctgccgcac ccgttcgggg aactccggct gctctttgaa ccgcgaagga
     1861 tcgaatacct ccgcccaggg gttctcgcgg ccccggatca ggtctgtcaa gatcatccct
     1921 gccgccgtgc cggcagccat cccccacttc ccaaagcccg tcgcgatgta gatgttctcg
     1981 tgcccctcgg cgatgcggcc gatgtagggg acgcggtcgg ccgtgatgta gtcctgcgcc
     2041 gaccagcggt agtcgacgga cctgaccggg tagaccgacc gggcgtacgc ctcgaggtcg
     2101 cggtagtgct cccgggtgtc ggtcaccgtc ccggtgtcgt gggccgcgcc ggtgacgatt
     2161 acgaggtcgt ggtcgccggc gggctgcgat cgccaggagt ggaccggtcc ttcggcattg
     2221 atgaggattc cgttcgggaa cggctcatcg atccggaggc cgagcgcata ggaccgtgat
     2281 gggtgcatcc gggcgaagta tgcgccggga cggtcgtaga tggggtagtg cgtggcgaga
     2341 accgcgtagt cggaggagag cgagccccgg ttggtcttca ccgtgacgcc gccgggctgc
     2401 tcatcgaggc ctaccgccct cgtcgtctca aagacgtaac tcccctctcc cgggaggnnn
     2461 nnnnnnnnac cgccccgtag gtcctgcccg gcaacgggac gtcctctaca aatgtcgcca
     2521 ggagcccgag gctccgtgct gcgtccgcct cggccgcgac agagtcgcgg gactctgccg
     2581 actccgcata ggtgtaagcg ggtttgcgct cgaaactgca cgggatgtcg tactcccgga
     2641 cgagagacgc gatcgtctcg atcccggcct ggttcgcacc ggcatactgc cgggcctgct
     2701 cctcgccgag ccggtcgata aggtccgcgt agatgagccg gtggagcgag gtcaccttcg
     2761 ccgtcgtata gccggtggct cccgtgacga tccggcgggc atcgatcagg gcgacggtca
     2821 gccccgcctt cttcagcaga aatgccgtgg tgatccccgc tatcccgccg ccgatcaccg
     2881 tgacgttcac gtgcccgtcc ccgtcgagcg gcggaaaccc cgtctccggt gaggttgcta
     2941 tccagtacga ctcagctgtt cccggcagat cgttattccg ttcccgcgac tcacccattc
     3001 ctcttcgcct cccggctgat gggtgccggg gcacctatat aacggttatg caggaacagc
     3061 agggaggccc ctgccgttac gcataagaac ga
//
LOCUS       DAJB01000005           25028 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65022, whole genome shotgun sequence.
ACCESSION   DAJB01000005 DAJB01000000
VERSION     DAJB01000005.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 25028)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25028)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25028
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65022"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    7602..7682
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11576..11614
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4461 a         7963 c         7637 g         4845 t
ORIGIN      
        1 tgattcctcg tcccgggtcg atgctcccat attcatgccc tattcaacag aactgaaatt
       61 tcgtagtttc gcgtgaagca atccatggtg cagatcggga tatggtccca cacagaaggc
      121 aggccgtgag ggatgcgaag ggcgggaaag gaaattcatc gagttaaatg acaacgagta
      181 ctagcgcgac agaaggatac ataagatacc ggcaaaccgc cggcgaaaga aaaaaagttt
      241 agccgaagag ggcgccgaga ccggccatgc cgctctcttc ttcctcttcc ttcttggact
      301 cttcctctgc ctcttccgcg gccacgggtg cagcggctgc aggggcagcg gcggctacag
      361 cggcgacagg tgcgacggcg gccttgctga tggcctcctc gatgttcaca ccctcgagtg
      421 cggcgatgag cgccttcacg cgggcatcct ggacggcgac accggccgcg ttcaggacag
      481 cagtcacatt ctcttcagtt acgtctttgc ctgcgttgtg caggagcagt gcagcgtaga
      541 tatactccat agttcattca cctttggttt tttataaata ttagccgaag agggccccga
      601 gaccggccat gccgctctct tcttcctctt ccttctcctc ttcctcggct gcgggtgcga
      661 ctgctgcctc tgctgcggcg ggtgccgcag cggctgctgc cgaagctgcc ttcagggtcg
      721 cctcatcgag ctcgaagccg tggtcttgcg ccgcgagcgc gacgaccagc atctcccgct
      781 gtgcccgccc gatgatcagg tcgacgatgt ccttctcata gatgctcgca tcgacgccca
      841 cgttccgggc ttcgcgcacc gccctgccga tgatggcact gacggtcagc ggggtcgcga
      901 tgacggcatt gaccgagagg ttgaacgcct gctgggcagc aagcacgatg ttgttgtagt
      961 aggcctcttc atcgatagcg aggaggtccg gcgtgaagat ggtgttcttg tagtatgcgg
     1021 cctgcaggag gagaccgaca tccatcggct tgatgccgag tttcaccagg gcatcggcaa
     1081 ccttcttact gatgacctcg cccttcttca cgaccgtctt cgtctcacga atcttgacct
     1141 ttccgccttc gatggctgcg ggaataccca cctgctgaag ctcgccgaca atggggccgg
     1201 gcttgaaggt ggttggaccc ttcgggacga cgatatcctc cggcgcagtc tcgccgggct
     1261 ttgccgccat cttcgtcttg gtctgttcaa gcagtttgaa gagcttgaaa gggttctcgt
     1321 tcgtgaagat catggcgctc tggccctcgg cgtgctcgtt taaagccgcg acattgccgc
     1381 cgagctcgtt taaggcgtgc tcgatcagcg tgttccgggc catcttcact cgcgccgtac
     1441 cgcggaggtt tcgccggata tgctggatct gtgaggcagg aatgccgtac atgtccacaa
     1501 cgccgacgag cgtgtgctcc gcgataccgc gcttgatctc gtctacttcg tccttcttcc
     1561 acctgggcag gtggtgtgtg tagagtgcca tcagatcacc ctcactgccg gccccatggt
     1621 cgttttcacg tagaccgagt ggatgttcat cgtcccgctc tccaggacgg cctcgaccct
     1681 ccggagaacc gcatcgatat tctctgcgat ctcagtgggc tccatgccgg aggacccgac
     1741 cttcgtgtgg aagactttct tgtccttcgt ccggatcttt acggaacttc tcaggcgctg
     1801 aacgatgggc ctgatatcca ttccctgcgg aatcggcata ggcattcgcc cgcgcggacc
     1861 aagtctgaca ccgaggtaac ggccgacgag aggcatcatt gccgtctcag caaggaagaa
     1921 ccggtactct tccgccatct tacgcgcttc ccggggttct cccccgagcc gctcgatctc
     1981 ctcaggtcca atgataagtt ccacacccgc atcttttgcc tgcgtggtga tgtcgccctt
     2041 cccgagaact gcaatcctga cgttgtcaaa accgttcggg aggagaatcg tctcatcgat
     2101 acgattcttg ggctgggaca tatcaatgtt cttgaggttg acggtgatat ccacgctctc
     2161 cttgaacttc cgttccggag ccttctccag tgccgtcttc acggctttct gtatactggt
     2221 tttatcaacc atcctttgcc tccatagtac acgacctcat gatcttctat ggttgtctga
     2281 catctatgcg acgagcacgc tgtcgtattc cccgtcattg atggccgaga tcatctcgcg
     2341 gggtttctta ccctcgacgg tgacaccgac actcacgcac gttccaacga cctctttgac
     2401 tgccgattta agatcgtacg agagcatatc atcaaatttc atacgggcaa tacgaacagc
     2461 ggcctccagc ggcaggtttc ccgccaccaa agcacccggc tctgtcgaac ccttcgtgat
     2521 accggcctct ttcatgacga gagccgtggt gggcggaatg cccacttcaa tcgtgaagtt
     2581 ccgcttatcg tcgaccgtga ccgtcaccgg tacttgcata ccgttgaaat cgaccgtctt
     2641 cttgttgatc tcttcgacga ctgccttcac gttgataccg agaggtccga gcgcagggcc
     2701 cagaggcgga cctgctgttg ccttgccacc gggtactaat acctcgaccg tttctgccat
     2761 acaagttctc cagcttattc ctcaggggaa tagttgcctg ggttatctaa ggtcatcgta
     2821 agaggattta aagtatggtg ccccggggtt ctgctccggc accccggacc ccgcacgagg
     2881 agatgtaccc aaccgatgcg aatcgtatcg tcgatggtgc aacgacccgt gcccgggcaa
     2941 aaagaagagt gaagggatat tgttcagttg gcgtcttccg accgctccac gacgcggacg
     3001 gagtcgccac gaaccgtgat cgggatgggg accatactct cgtacaattc gaccgtgatc
     3061 tcttccttcc cggtatcgat acgcttgacg accgcctttt cgcccttgaa cggtccggcg
     3121 acgatctcga cgatggtgcc ctcggtgatg ccgctgacca ccggtttggg gaccaggaaa
     3181 tgctcaacct ccgatagaac ggtcgagccc tgcaccaccg cgcgcgcgtg ggggatcaac
     3241 tcgaccagct gctctatccg ggcgatggag tcggggctct ccacaaggac gtagcccttc
     3301 agttcctcgg gaaccatgac agccatcaca tggatgtctt tctgctccct cgtcaccttc
     3361 tcgatgttgt cggctaccgt ccgctcctgc tttgcggtcg tcttgatcgc gtatactctg
     3421 tttgcgctct cagccatatc catcatttgg gcaataccag caggatctcg tatataataa
     3481 agccgacaag gccgatgagc atgatcccgg ctgccgccac gatggcgatc ttggagaact
     3541 cgtcgcgagt aggcgtccgt gccagcttca gcacccgcca gtacttcttg aagagctcct
     3601 cctcgatctt gaacgacttt atttcatcca acgtctcttt ataatccatc ataccggctc
     3661 acttcaggaa gtcgatctca aactgtttca tcatgcgtgg catactcttt tcgtttctcc
     3721 catatatttg tggtgaccgc acaccggtga ccacgagcag cgtccgcatt ttgccctgca
     3781 tatcggggtc gacctgtgct ccccagatga tgcgggcatc tgggtcgatg cggtcgtaga
     3841 cctcctgaat gacgccttca gcctcagaca tcgtcatatc gggtccgccg accacgttga
     3901 cgagtgctgc gctcgccccg gagatatcga cgtcgagcag cggggagcgc agcgccttct
     3961 tgaccgagtc ggcggctttg tcctcactgt cactctcgcc catcccgatc atcgcgacgc
     4021 ctccccgttc catgacggtc cgaacatcgg cgaagtcgag gttcacgagc ccgggcatcg
     4081 tgatcagctc ggtaatcccc ttgaccgccc gcatcagcac ctcgtctgag accttgaagg
     4141 cggcgtggag gggaaggcgg ggcacgacct cgagcaggcg gtcgttgggg acgacgatga
     4201 cggtatccgc cacttcgcgg agcctttcaa ggcctgcctc ggcgttctgt ccccgtatag
     4261 cgccttcagc cgtgaacggc agggtgacca cggcgatggt cagtgcgccc tcctcccggg
     4321 cggacttcgc gaccacgggt gcagagccgg tgccggtgcc gcccccgagt ccggtggtga
     4381 tgaagaccat gtcgcacccc tcgacggccc gcttgatctc atcctcgttc tcgagggccg
     4441 cctcttcgcc gacctggggg atcgagcccg caccgaggcc gcgggtccgc tgccttccga
     4501 taagaatacg agtctccgcc cgggtcctga tgaggtgctg ggcatcggtg ttgatggcga
     4561 tcagccgcgc cccattgatg ccttcctccg acatccgggt gatcgtgttc gaaccgccgc
     4621 ctccgcaccc gacgactgcg atctccgtcc ggagctccat gaggatatcc tcaagatcct
     4681 tgtcgacctc tatgggctca gcaaagcgct gatcctctcg tgcccgtgaa agtgcctctt
     4741 ctacgatgga tttcatatgt atctctccaa tcccgggata tgtatccgct tcacgctggt
     4801 tgtctgcact gcatcaggtg taatccttcg cccctcgatc tcgaccgctt tcccgccggc
     4861 aagcatggcg aagagcggcc ccctgggaac cccgtgcctg cgcgctttct cggggtcgaa
     4921 ccgcaccttc cggaggacga ggtgattacc gtcgataaca gcgtcttcac agataagtaa
     4981 gagttttacg cacagggtag ttatatcact tgcgagttgg gacgaccctt tttcaaagca
     5041 gatgaacgta gataagacct ctgtagatcc tttcgagaga tgagcgaccg gcaattgatc
     5101 gaggccattg agaaacccgg atttatcgtt ttttactgtt tcttcgacca gatccgggat
     5161 aatctgcacc ggaacagggg tcccttctcc agctagaccg tggatgcgga tgcgtgaccc
     5221 cggagcgatc ttttcggcga gagtcctgac cctgaggtag gtggcccatt cgaggcccga
     5281 gatctccagg atctccgact cggagaggag gaggattccc gcatcgtcga gcatccgctc
     5341 gacccgcccg atctggtcgg tggagagtga ttttctgtcg atgtaggcgg ccacggcact
     5401 gctcgcctcc tgcatcctcc ggagaagatc ggggttgatg gcgccgatct gccgggtcgg
     5461 cacgatgtgg ccgaaggcac cccgggaaga gagcgcgatc ccggtctgcc gcacagcgta
     5521 gtgggtcccg ccgaaaccga tgaggttgat cgtctccagt ggcgccgctg agaggatgct
     5581 ctccgccacc gccctcccgg cggccgggtc ggcccattcc gtggcggtgg atccgatctc
     5641 gacgaagagc gacggtgtcg cgagcgccgt cgggccgtgg tgcgtcacct cgtaggagac
     5701 ccggtaaccc tccggggccc ggacagcgag atttcgcagg atagcgtgca tccacagggg
     5761 ggctgccggc acgagcgttc ccggctcccc accgaggtcg gcggtctcgt agttgccggt
     5821 cacgtgcacc gtcagggcgg gcgtcggctg cgcactcgag tgccgggaaa tgaaaataat
     5881 gagatcggca tcgacctccc ggtcgatccg gtcctggtag atcagccttc ctgcgacctc
     5941 gtaaaacgtc agcgtatggt ggtccgcaag cggccaccgt ctgtctcccg cgagcagggt
     6001 ccggaggtgg tcccggatgt tctgccccgc aacgtccact ctcgagttaa tcagcgcgac
     6061 ccgcatctgt acatacatgg gtgagcggta tgctaaatgg tagtgcttga ctggcgcgat
     6121 agtaaatcct atcttcctgc agacagacca tgtgagtact atggacgaag agaagataca
     6181 gaaagcattc gaagcgtacg gcataacgga cgagatcacc tgcccgcagg cgttcgagat
     6241 atcggagaag tgcgatatcc cgaagatgga tatcgcccgg tactgcaacc agcgcgaacc
     6301 gagaattaag ttccgcggct gccagctggg ctgcttcaga tgagcctctt cctcgaggtc
     6361 gtttccgtca gcgaggccgt aaaggcggtg caaaagatcg cacccctgcc cgggtgcgag
     6421 gaagtcccgc tcgaagacgc cctccaccgg gtcctcgcaa gggacgtccc tgccgatatc
     6481 gacatcccgg ggtttgaccg ctcgacggtc gacggttacg cggtcgccgc ggccgagacc
     6541 accggggcct ccgaggctat cccggcgatg ctccagtgcc ggggccgtgt cgggatgggg
     6601 agcgctgatg cggagagtgt ctccccgggg tcgtgcctct atgttcccac cggcggcgct
     6661 ctccccgggg gtgcggacgc ggtggcgatg atcgagcaca ccgagcagat cggggacgac
     6721 atcctggtgc atcgcccggt ggcacccggc gagaacgtgg tcttccgggg tgaggacttc
     6781 aaggccggaa aagcggccct ggagcggggc cgcgtgcttt cgccccggga gatcggcgtc
     6841 gccgccgcgg tcggcgctga ccgggtcacc gtggtgacga taccaaagat cgggatcgta
     6901 tccacgggga acgaactcgt cccggtcgtc gagacccccg ggccgggtga ggtgcgggac
     6961 gcgaactcct acctcgccgg cgccttcgtg acggagcggg ggtgccaccc ggtctacttc
     7021 gggattgtca gggacgagcg ggcggtactg aagagagcgg tctcttcggc gcttgaacgg
     7081 tgcgacgcgg ttctggtctc gggagggtcc tcgaaggacg aacgggacaa caccgcagcg
     7141 gtcatcgcag aactgggcga ggtgctggtg cacgggatcg ccatcgcgcc ggggaaaccc
     7201 accatcatcg ggatggcgcg gggaaaaccg gtcatcgggc tccccggcca cccggcctcg
     7261 gccttcgtcg tgctcgtcgc cgtcgtcgac cacctgctcg cggcgatgcg gcagaccacc
     7321 gtatcccggc ggaccatcca cgcgcggctg acacagaaca tcccctccgc ccgcgggcgg
     7381 gaggactacg tccgggtcag tacgcgcgag ggcgaggcca cccctgtctt tgggaagtcg
     7441 ggactcctca acacccttgt gcagagcgag gggctggtgc gggtcccggc gtcgagcgag
     7501 gggtttgaga tcggcgagga ggtggaggtg atcctgtggt gaagcgctac ctctcggtca
     7561 taacgcttgc agaggcactg gcgctcgtgg agcgctcctt tnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nncctcccgg atgcggcgag agtcaataca ggcaacatca tcccgcccgg ctacgacgcg
     7741 gtgatcatga tcgaggacgt ctgggtcgac ggcgagacct acaccatccg aaagccggtc
     7801 agcccctggc agcatatccg ccccgtcggc gaggatatcg gggaatccga gatgatcctc
     7861 ccgtcacatc accggatccg gccgccggac ctgggggcgc tcgcaaacta cggggtcacg
     7921 gacctcgccg tccagaacgt ccgcgcgggc ctgatcccca ccgggagcga gctggtgccg
     7981 gcaggcgtaa tgcccccacc ggggaaggtc gtggagagca acaccctgat ggccgcggcc
     8041 gtccttgcgg aggcaggcgt ggagacgcac cgctacccga tcgtcccgga cgactacgac
     8101 ctgatccggg acgccgtccg cctcggcgtc gctgagaacg atatcctgct cgtctcggcc
     8161 ggctcgtccg ccggaacccg cgactacacc gccgatatca tccaggacct cggcgaggtg
     8221 ctggcgcatg gggtcgccat caaaccggga aaaccggtga tcatcggcag gatcgacgga
     8281 aagccggtga tcggtctccc cggctatccc ctcgcggcag cgaccgtgct ccgggagctc
     8341 gtcctgccgc tcatcgcccg ctacggcctc cccatcccgc gaccggagga cgtcgccgcg
     8401 cggctgacaa caagcctcca gtcggacatc gggaccgacg agttcgtcct cctctcggtg
     8461 gggaggatcg gtgaacgctg ggtggcggtg ccgcaatcgc ggggcgcggg cgtccagatg
     8521 agtgcagtgc gggcaaacgc atacctgcag atcccggcac ggaaagaggg tgccgaggcc
     8581 ggggagaccg tgaacgtccg gctcaccgtc ccccgttccg aagcggagga ggcggtgctc
     8641 gtcaccggca gccacgaccc ggccctcgac tatctggctg accgggtgag accgcgcggc
     8701 gtcgaggtac actccaccca tgtcgggagc atgggcgggg tgatcgcgtt aaagaagcag
     8761 gagtgccacg cggcccccat gcacctcctc gcccccgacg gcacctataa cacccattac
     8821 ctggagcgtt acatgcccgg cgccgacctt gccctcctct gcgtggcaga gcggcagcag
     8881 ggggtcatct cccgcgacgg gctaacattt gacgatctgc ccgggcggac gttcgtcaac
     8941 cggcagaagg ggtcggggac cagggtgctc cttgaccact gcctcgcaga gcgcggtatc
     9001 gacccggcgt ccatccccgg atacggtcgc gaggcgacga cccaccttgc ggtggccctt
     9061 gcagtccgga ccggggaggc ggacgcaggg atgggggttt acagcgccgc aaaggcgctc
     9121 ggtctcccgt tcgtcccggt ggcgacagag cgctacgaac tcgtgatgca tcgcgcgatg
     9181 ctcgacgacc cccggattgc agccctcgtc gagacggtct cgtcggaggc gttcaagcag
     9241 atcctccggg acctcggggg ctacgagacg gacgagacag gcgtcctgcg cgaactgcga
     9301 ggatgaccgc ctcctcgggg gtggcgcagg ccatcacccc ctctatcttc caggtaagga
     9361 ggccaaagac cggcttaccg gtcttgaggc tgactgcgat ctccgagagc gtcccgtaac
     9421 ctcccccgac ggcgatcacc gcgtccgccg agtgaacgag gacaacattc ctcgcgtggc
     9481 ccatgccggt acggatcacg acgtcgagat aggcgttgcc gtccccggtg tccggaagga
     9541 tgcccaccgt cgtcccgccg gcctcttttg cccccctgca ggcggcctcc atcaccccgc
     9601 cgaggccgcc gcaacagacg gtctcgtgat tgccggcgat gaggtagccg atagtctctg
     9661 ccgcctcgga ctcttcgtcg gaacagtccc ctcttccgat gactgcgatc tgcatggggg
     9721 agagtcgtgc gttgcctata aagacctgac tgggtttgct cctgcgatgc cggcagtcgc
     9781 aacccgcacc tctggcgcct tcactccatt acctgcacct ttaatgctcg aactccttcc
     9841 ccaacgggga agtcatctcg atcggaatgt cgcacaaaac gctttgtccc acgagaacaa
     9901 acggtacagg agagtgtaaa tatgagggat ttgcggtgaa agaagtcacc agcagttata
     9961 acccgcgcga gatcgaggcg ggagtccagg attactggaa gagggaaaat acctatgcac
    10021 gcgtccagga cctgaggaag gatggaaaag cattcttttt cgtcgacggg ccgccgtata
    10081 ccacgggcca catccatctc ggcaccgcct ggaacaagat cataaaagat gccatcctcc
    10141 ggtatcaccg gatgcggggt gcgaacgtca tcgagcgggc cgggtacgac atgcacggtc
    10201 tccccatcga ggtgaaggtg gagcaccagc tcgggttcac ctccaagaag gatatcgagg
    10261 agtacggcat cgccgcattc atcgagcagt gccggacgtt tgcggtgacg cacatggaga
    10321 tcatgagcga ccagttccgg cagctcggca tctggctcga cttcgacaac ccctaccaga
    10381 ccattgatcc gggctacatc gagtccgcgt ggtgggctat ccagcgggcc gacgagcgtg
    10441 ggctcctcga acgcggccac cgggtcgtga actggtgccc ccggtgcgag acggcgatcg
    10501 ccgattcgga ggtggagtac tgggacgaga ccgacccctc catcttcgtc aagttcccgg
    10561 tcgtcgggcg cgagaatgaa ttcctggtga tctggacgac gacaccctgg acgctcccgg
    10621 cgaacgttgc ggtggcggtc aaccccgcgt tcacctacgc gcgggtgcgg gcgaagaaag
    10681 acggcggcga agaggtcctc tggatcgccg atgaactcat cgagcccgtc ctgaagatgg
    10741 gccggtacca ggacttcacc gtcctcgaac gactgagcgg gagcgacctg gtcgggatgg
    10801 agtacgcgtc cccgctcgcc gggcacgtgc cgcaccaggc ggagatccgg caccgggtcg
    10861 tggcggccga ctatgtcacg ctcgagaaca ccggcctcgt ccatatcgcc cccgggcacg
    10921 gatgggacga ctacctggtc ggcatccgcg aagggcttga ggccttctgc ccggtcgacg
    10981 ccggcgggtg tttcaccccg gcggccgggg catttgcgga catgtacgtc cgggacgcaa
    11041 acgacgtcgt catcgacgcg ctcggcggct atctcctcgc caggcggacg atcactcacc
    11101 gttacggcca ctgctggcgg tgcaagaccc ccatcatcta ccgggcgacg gcacagtggt
    11161 tcctgaaggt cacctgcata cgcgactcga tgcttgagga gatcgcgaag gtaaagtggt
    11221 accccgagtg ggcgggaagc gcccggttcc acgatttcgt caaggagtcc cgtgactggt
    11281 gcatctcccg gcagcgctac tggggtatcc ccatccccat ctggcactgc gaccggtgcg
    11341 acggttacac cgtcatcggc accgtcgccg atctcgagga acggtccggg acaaagattg
    11401 ccgaccctca ccgcccctac gtggacgcgg tcaccatccc gtgctcctgc ggcggggaga
    11461 tgcaccgggt agccgacatc ttcgatgtct ggttcgactc ggcggtcgcg tcatgggcga
    11521 ccctcgggtt cccccggcag cgtgaggcct tcgaccgtta ctggccggcg gacttnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngacgcc gaagggcgca agatgagcaa
    11641 gagcgtcggg aacgtagtga cccccgaaga ggtgatgaac cagttcggcg tcgacgtcct
    11701 ccggttctac gtcctctggg cgaacgcccc ctgggacgac ctgaagttca actgggatgg
    11761 cgtgaagacc atccaccggg cgctcaacat cctctggaac gtctaccggt tcccgctccc
    11821 ctatatggtc ctggactcgt ttcagccggc atccggggag accgatcgat gggacgggtc
    11881 gttcgtccgg tctcacatcc gggatatgcc cgaagaggac cgctggattg tctcgcgggt
    11941 aaacacgctc atccggacac tcgacgagga tatgcaggag tatcaccttc accgggagac
    12001 ccgggcgctc gccgccttcg tcctcgagga cctctcccgc tggtacgtgc agctcgtccg
    12061 gccccggatg tggctcgatg aggactcacc ggagaagcgg tatgcctacg agacctccta
    12121 ctacgtcatg cgtcgcttga tagcgctcct cgcgccgttt gccccccaca tcaccgagga
    12181 gatctacggc aacctccgcc aggaggggga cccggagagc gtccatatgc tcgactggcc
    12241 ggaggcagac gacctcctgg tcgatgcgca tctcgaggcc gatatggagg tcatccgatc
    12301 gttcgacgac gcggtcgcca ccgcccggca ggccgggaga cggaaactcc gctggccggt
    12361 tgcggagacg gtggtcgtca ccgaaagcgt tgacgtgaag gcagccctcg aggacttaaa
    12421 caccctcgcc ctgaaccggg cgaacacccg gacggtcaga gtcgccttcg ggacatggga
    12481 ccgcatcctc tggcaggccg agccggtgat gcgggccatc ggcccggagt tcggcaggga
    12541 ggggccgaag gtgaaggccc tgatcgagca ggcggacggc accgtcctga aggcggctat
    12601 cgagcgggaa ggaaaggccg aactcgacgg ctacgagatc accgcgcgcc acgtcacctt
    12661 cgccgaagcc ctccccgagg gtgtctttgc ggcccccatg aaggacgcga ccgtctacgt
    12721 ggatgtcacc ctcacgccgg cgctggaggc cgagggttac gcccgcgagg tgatccggcg
    12781 gatccaggag atgcgccgcc agctggacct gaacgtcgac gacttcatca tcgcggccgt
    12841 tgatgtcgcc gacgagcggg tggcctcgct tatcaaaaaa gaggagtggc agaaagagat
    12901 cgccggagag gtgcgggcgg cagccctcac cgtccgccac accgacggag agagacccgc
    12961 agagcccttt gcgctcgaga aggactggga tgtggaaggc gtgcagatgc agatcggcat
    13021 ctcacgcgcc ggtgagtgac cggatcagcg ccgccccttc aacggttgag aagaggggcg
    13081 tctcctccgg ctcccggata caggttcgcg cggtctggac ggtctccccc gtcatggggt
    13141 tatacccctc ttttatgcac tcttcacggt agagaagcgt cccccgccgt tgccaggccg
    13201 gcgttgccgc caggttgacg ccccgctcaa acatcatctc gtgcatcgcc tcggactgca
    13261 gcccccggag cgcggcggcg gcctcccggg agctcattcc ctcgctgacc agcgcgctct
    13321 ggcagtaggc gttgatgtgg tttcgccacg cctcgttctg ccgcgagata aggtactcga
    13381 ccgcaaactc ctctgttgcg gggatggtcc gggcatcaaa cgcaaggggg ttggcgcacc
    13441 caagcttgat cgtcagcacg ctcgccgcaa ccgcggcagt cacggagtcg atcttctcca
    13501 cccgtccgga gaacggcagc gtcttaaagt aaaggctgat ctcgtcggag aaggtgtagg
    13561 cgaacgcagg cgtaagaccg ctctcggtga gaaggtagcg gcagaccgac cgcatgctcg
    13621 cgcaaaaagc aggatcgaac ggctttttga gatcgagcgc acgggcaaga cggtgaaagg
    13681 cccgaccgtc gagcctgacg aaaacggggg ggaaaatggt gagattcgag aagatctcac
    13741 ggtctctggc gtccatattc tatcggtaag gatcccggga taggccggag atcataatta
    13801 cgcttctttt gccggggact tgaagatccc ggggatgtgg cggacgatga cgatatctcc
    13861 gatgctcttt acgatgcgga agggtatctg cagtcctgcg tagcccttca cctctccgat
    13921 ctcgggattg agctcgccga ttgcaaggga ggcgatcttc ttctggtcca catcgagaac
    13981 gacatcgtca acgttcccga taaagacggc cttatcagta tagacgcgca gtccgaacat
    14041 ctctgtgatc tgtgtttgca tcggtatcct ctgaaggtat attactataa atgataaaaa
    14101 gatactcctt ctcatggaag cgtcgctaca gatcgggaac ctggccggga ttccggtcaa
    14161 actgcactgg agttttctcc tggtgatccc tctctttgca tggatcatcg ggagccagat
    14221 cctgctcacg accgagttga tcgagactct cttcggtgtt cctatcaacc cgacgctgat
    14281 tacggcggga ttcaacccct acatcctcgg ggccgttgtc gcgctcggcc tctttgtcgg
    14341 tgtcttcatc cacgagatgg cccattcgct catcgcgaaa gcaaagggaa tcaagatcca
    14401 cagcatcacg ctcctcatcc tcggaggtgt ctcccagatg gaggagacga tgccggaccc
    14461 aaaggtggaa ctccccatgg cactcgcagg cccgctgacg agccttgcgg tcggcctgat
    14521 ctccggtgcc ctggtctacg tctttgcggc ggtcgatctg gatccgggcg tcgccggggt
    14581 tctggtcttc accttcggct acctcggtct cttaaatatc ctgctcttcg ggttcaacct
    14641 tctcccggcg ttccccatgg atggggggcg cgtgttgcgg gcctggctcg ccaaaaggat
    14701 gccgctctcc cgcgcgaccc ggatcgccgc cgacgtagga aaggggtttg ccgtcctctt
    14761 cgggatcgtc gggctcctgc tcttgaaccc catcctgatc atcatcgcct tcttcatcta
    14821 catcggggcg aaccaggagg ccacgttcct ccgctacaac atcctgctgc aggacgtcac
    14881 cgtagcggac gcgatgagca ccccggtcgc caccgtagaa ccgacaatgc cgctcccccg
    14941 gttggttgag atgatgtacg agacaaagca cctcggcttt cccgtggtgg aacggggttc
    15001 gctcgtcggg atcgtcgccc tcgccgacgt ccacaagatc tcgccggccg accgggaggc
    15061 gatgcaggtg cgggacgtca tgactaaaaa cccgaccacc ctcccgccat cggcaccgct
    15121 catcgatgcg ctgaggatca taacgggcca gaatatcggg agggtaccgg tggtcgcaga
    15181 cgatacgctg gtcgggatcg tcacccggac ggacgtgctc cgggtgatgg agctgcggga
    15241 ggagaggtag ggggctccgt tccgatcgga acatcgcata accccacgca cggctttcga
    15301 gggggtatcg ccacgcactg cccgtccccg ggggggacgg aacgacttcc ccgcagggga
    15361 aggagtttga gcaccgaagg tgcgagtgag gagcgcgaag cgcgaagcgc gggcggtgga
    15421 gggttacaga tatgcccaat gcgatggaga caggggaggg gggcgtgccc cccctccccg
    15481 tcagccccac ccccaatggc gatatcccca cagtccagtg taccgggctt ttccatcgag
    15541 taggttgctc aggtctttgt agccctgaat gagttctccc aactacgcct catgttccgg
    15601 caggttgcag ttgctcagga tcgagatttt cagaacctgt tgaacctatt cccacgagcg
    15661 caggacggga agatcggagt tcaagcacca ccaggtgcaa acacgactcc ctgaagaagc
    15721 aacagccagc acagggcatc cccccctaaa acctcatcac gtcctcgatc ccagtctccc
    15781 cggcgaccgc ccggaactct tcaaggcttc caaaaggccg gcgggcggcg atggttgcgg
    15841 ccctcttctt accgacgccc gggatccacc ggagcgccgc taccggcagg gcgttgacct
    15901 ccaccgggca ggggagggcc gtcaccgacc gcatccccca gtccaccacg acagtgtcga
    15961 gcacggtccg cgccgggagt tcgaggggta tcccgaccag gatggggtat gagcccatct
    16021 gccgcccgaa tgacagtttc cccgggatct cgacgacgac acccgaaagg atggtgccag
    16081 cgggaaagac ccggcgaagc atcggctcgt cgaactgcgt ccgggcccac tccttgaagg
    16141 cccggaaccg ggaatcgtgc ttcccgaggg tgttctcgtc gtaggccgcc gtcccttcaa
    16201 acggcatcag ctgccggatg ttcacccgcc ggacgagaag actggcatcg agcacccgct
    16261 taagaaacgc ctcgttcaga tcgaaggtcg ttgccgtctc ccccgcgaga ccgacgatga
    16321 agtttaagcc cggcaggagt tccgggacgc cccccgtccg ctttccgccg acatcgttca
    16381 cgatctcgat cgcgcggaag acgtcgtccg gcatggcctt gaggttgttt gccgcgacaa
    16441 ccgccggatc ggcggtctcc ataccgaagg ccgcggtgtc gccgggagtg tggccggcca
    16501 cgattgccgc gagcgccgcc cgggctgcgt cctcgtggtg ggcgatggtg cccgggttga
    16561 tgttgtcgat gtgcagggtt ttgaggtccg gggcgtttgc cctgatggac gagaagagtt
    16621 cctcaagcac ctccgggcgc gggacgggga actccccgcc gggactctgg taggcaagga
    16681 ggtccggctg tcggccgagt ctgaaatggc aagcacccgc cgcacggagc gacgccacct
    16741 cctctgcaat cccctcgatg ctgcggtagc gcgggagccc gtagaagggc tcggtgcaga
    16801 aggagcatcc cccgaccgtt gcccgggagc acccggtcgc ggtctcgagc tcgcacatga
    16861 cataggggaa cgccgggtgc tcgccgatga tccctgcgcc cgccgcgcac caggggtcgg
    16921 tcagggagta gtcgcccgcc ccggcgggtt cgccgcccga gagccaggca tccagcgcga
    16981 ccgcaggcga ccccgagagc gtgacgtcga acccggcgat agcctgccgg attgccttct
    17041 tcccgccgcc ggaagcatag ccgaaggcga tcgggccgcc gatcgcggtg gtcggctccc
    17101 ggagcaggat cccgatctgc tggacctccg tgagggttgc aggcttcccc ccgatgtagg
    17161 cgccgggcac ggtcaggccg gcgatcatca ccacgaggtc tcctctgccg ggcgcggcaa
    17221 ccagagccgg gtcggaccgg acctgatcga tggtgacgta gcggggagcg tacccgtgct
    17281 cgatcaggac gccggcgacc tccctgatgt agggggagat gtagggcgca acaccgaggc
    17341 acgcgggctc gtcgacgtag ccgtcgatga tgagagcgct agaggtcatg gccaatcaga
    17401 cggaggagtt cccccgccca gtacagtttt ccccgcttga cggggtcgac gtttttgtcg
    17461 gcgaggtcga atcctatgat ccggacccgc gccgccccga gttcgtcggc ggcaaagacc
    17521 gcccggtcgc cgtcggtgaa cccgccgaag ttgtggacgt gaggcagggg ggccgcctgg
    17581 gtggtggcga cgagcgggcc cgggaggaga gggacccagt ggcggaggag cgggatgttg
    17641 tcgccgtggg catgcacgac catcaccgtc ccctgccggg agaggtcgat gaaggcgtcg
    17701 gtcgccccgt caaggtcggt gaagaccgcg tcgggccgga tcccgtggtc cgcaagcacc
    17761 tcggcggcgg catcggcggc gagcaccgtc ccctctaccc ggtcaagttc ctccaggagg
    17821 cagggagcgt tcccgcagac cgtcaccgct ctgccccgga tgagcgcctc aagcagtccg
    17881 acgtcatccc ggtccgccag atcgccgagc agccgggcgg ccgcctcgtc tgcctcgcgt
    17941 tcaaacccga aatattcaag aattgcagta tagtgaggtt cccagtccgc aaacctcatt
    18001 gcttatcctc gttgtccagg ccgacgatgt tggagaaccg cttcaggatc ggatcgatga
    18061 tcaggccgag caggccctcc ttctcctttg ccatggaaaa gtaattgagc ggtatcatcg
    18121 cagcggccat cgtggcgtgt ccgcccgcct caccgatagg cccgaaagcc tcggccatca
    18181 cgttcccgag atgaagcctg atatccttgt tcctggccga gatgatgata ttctggtcgc
    18241 tgatgccgta aaccagcgcc gtgttcacgc cctcgaggtg gatcagcata tcggccgcct
    18301 gggggagagc gtcgcggttc ctgatgtaac cgacgttgga gaagaggtag ccgctcttga
    18361 tccgccggtt ctggatggca tttccgatga tctccatggt ctcctgcgat atggagggag
    18421 aggtgatctt gtcgaggagg tccgagtcgg tcagagggag gaggaacgcc gcatagttga
    18481 ggtcctgggg ggtgacgttc ctcttgaagt ccctcgtgtc ggctctgatg ccgtacagga
    18541 gtgcggtggc taccgatttg ctcaccggga tatcgagttc catcaggtac tgggtcagga
    18601 tgctcgccgt ggcaccgacc cccggcctga tgtcgatgaa atcggcgacg gtagagggat
    18661 gctcgccgtt cttgtggtgg tcgatgatga tattgacccg ggtggacctg ggaagctcgt
    18721 tgttcgtgcc gggtgcggat gagtcgacca gggcgatata gttgcactct tcgaggatct
    18781 ggggagtgag acgctccatc ttgatctcga ggaggttgat gaacgcccgg ttctcctggt
    18841 gcccgatgtc cccttcgtag aggatgcggc acgtgagttt gttgcggctc gcgtgcctgg
    18901 cgatcatcga gagcgccatg gcgctcgaga tcgagtcggg atcggggttt aggtgggtga
    18961 cgatgcagaa cgttccctcc caggcggcaa gcatatcgta gagccgcagg gcgagcctcg
    19021 atgactggag tttccggatg tggtggatag cggtcctggc caccacctcc tgcgggtaga
    19081 ggacgatgtc ggcgccttcc tgctggagga ggtcgacgct gaccgggtcg gtagcgcgtg
    19141 cgatgacgta ggtcgccggg taccggttct tgacggtctt gagcgcggtc aggttggcct
    19201 cccggtcgtt ggcgaggatg aacgcgacct ccggaacggg cagaccctcc atgaggtttg
    19261 ggtcccggag gtcgcgaacg agcgcttcgt acttctgatc ccgaagatcc tccacccgct
    19321 tttcatcctt atcaaggatg atgagatctt cgttctcctg ctcgagctcc tctgcaacgt
    19381 tatagccggt gcttccgcag ccgaaaatca tatatttaat acgttctttc gaaacgttct
    19441 ggaccgcctc aggcatgcgt acaatggtgt aacttctcat agtattaagg gattccattt
    19501 cggcgatttt cgggagggtc ccgtaagatt tataccaatt cccatgctat actattaggt
    19561 cagatacatg ggcctgtagc ttagttcggc agagcgacgg actcttaatc cgtaggccaa
    19621 gggttcaaat cccttcaggc ccgnntaggt cagatacatg ggcctgtagc ttagttcggc
    19681 agagcgacgg actcttaatc cgtaggccaa gggttcaaat cccttcaggc ccgtcgaact
    19741 ctcaattctt tccatcgttt gcatccggca tctggtctcc agtgacgacc gcccggttcc
    19801 cgcaatcacc cccggagtga tttctccatt gcagacgggg cgcccgaccg tcaggtcaac
    19861 ccccatcggc ctccttccgc gccatggttt ctcacaggag accgattttc atgacgacaa
    19921 cggggttccg gaggcacata catgccgctg ctatataaac cctgctctga cagaaaaccc
    19981 gggggagcgt tgtgcggggg acgggatagc aggggacccc cggtttccca ccccctgccg
    20041 caggcggggc ggcagggtca aacgaaagag gattctcgat acgccgcgtt ttctcagggt
    20101 agtcccgacg gttccccggg caagagcatg gaccccggaa ggaccccttc gcccatcagc
    20161 tcgatccgtt tcccattgac ctcgatcgtt agcctgcctc cggaagggag cggccggatc
    20221 ccatcccgtc tctcgcatcc ccgatcagcg cccgatcttt aagagcatct cttcgagcgt
    20281 cgggtagtgc gactcgatcc gctcgatcag cgcgccgtct gccgcaagcg tcgcggtcgc
    20341 gcggttcagc tcctcgacgg agtgcgcctc ggcgaggtgc cggccgtctg aggcggcgta
    20401 gccgaccgac gtggcgaagg ccgccgggtc ttcgatccgg aagaagatct ggtaggtgat
    20461 cgagccgaag gtctccctga gttcgggcat ggtcccttgc gccaccaccc taccccggcg
    20521 caggatcagc acgaggtcgc aggcctcctc cacctggaag aggttgtggg ccgagaagat
    20581 taccgtctta ccccggtcgc ggaggccttt gacgtagtcg agcaccgacc gcgaggtcat
    20641 gggatcgagg ccggaggtgg gctcgtcgta gatgagcagc cccgggtcgt gcatcagcga
    20701 cctcgcgata gcaaccttcc gcttcatccc cttcgagagt tccccgatct tcttcccatc
    20761 cgcctcgagc gcgagccccg cgaggaggtc gtcgcgccgt ttccggatgg ctgccttcgg
    20821 cagatcgtag atctcgccga agaacgtgag gtatgcatcc gtggtcatcg tctcgtagag
    20881 ccgggactcc tcggggaggt agcccaggtt ctgtttcaga tcaagcggtt ttctcgcgac
    20941 atcgatcccg tcgatgacga gatttcctgc cgtcggcgag ataaggccgg ccatgatctt
    21001 taggagtgtt gtcttgccgg cgccgttatg cccgatcacc ccgagtatcc gtgcatcgtc
    21061 gaggtcgaag gtgacaccgt cgagggccgg gaagtcgccg tagtgcttga ccagagagcg
    21121 ggccgtgatc atctggtatc ccctatgtct ccgatgtcta ctaataggtt gctgcacaga
    21181 gatatggttc tccgacgcct tctggaatta ccatgagtct cgcctcctcc atccggacga
    21241 tcgctgtctg ggagatgaac cggtcgatga ccacgatggg caggcacgtc ctgccgctcg
    21301 cggcagggct gctcgtcctc ctggtgctgg tgacggtgtt tgctgcccag agtggcgtcc
    21361 atatgcagga cggcatctat cgcatcggca tcgacgatcc cgacgtcgcc cgggtcgtca
    21421 ccccggataa ccgctttgcc acctacctcg acgacgcccc ggccctctgg gagagcaggc
    21481 tcgcgtacga tatcgtcatc ctgcacggcg aggtctacgc tgcagatacg gataaaggtc
    21541 gggcggcctt aaaggcactc gaacgcgact acgagggtta cgtctcccac gtagccacca
    21601 gagagcctga tctcttcgcc gcctacccgc tctggatcga cctccagtac gtgaagagcg
    21661 agatcgactt cctggcgacc cagagcgggc agcaggtcgg ggctccggcg gatgcccggg
    21721 tgcctcccgc cccctccggc ccggtcgagg cggtgaccgt ccccccgtcg gccatgccgg
    21781 tctccgaaga cgacctcaga gagcacctcg cggaggcggg gggcggccac cccctcgacc
    21841 gctacacggg catcatctca ggcgaccccg cgatggaccg gttcaggaca ccgtcggagc
    21901 tctcggcgcc cctccccttc gatgcgctcg ttctggtctt cgtcttcatc ttccccctct
    21961 acttcacctc ccagttcttc atgatgagcg tgatgaacga gcgggtggga agagccggag
    22021 aggctctcct ctccaccccc gtccggccgt ccgcaatcat catcggcaaa gcgctcccct
    22081 acttcacgat catgatcctc atctcggccg cgatcaccct ctttgccggg gtgccgctga
    22141 ccatcctctt cccgctcatc ccggtcatcc tcttcttcct cgcaaacgcc ctgatcatcg
    22201 ggatggccgc ccggagcttc aaggaactct cgttcgtctc gatcttcttc tcgaccctcg
    22261 ccacgtcgta cctcttcttc ccgacggtct ttgcgaacac ccacgtcata agcatcatct
    22321 cccccctcac cctggtcgtc ctcgatatcc agggcgacgg gttcaccgcc atggagtacg
    22381 tctactcgac cgcgctcttc tttgcaacaa gcatcatcct cttctacgtc gggaccgtga
    22441 acttccggga ggagcggctc ttctcggaaa agcccctcgc atcaaggctc atggacttca
    22501 tctcgggcgg gatcagccgc gatcacccgc acctctcgct cttccttctc tccgccttca
    22561 cgataccgtt cgtcttcatg gcccagatga tgacgctcgt cctcttcttc aacatcccga
    22621 tgccgctctc cctcgtcctc ctgaccgtct ctgcggcatt catcgaggag ttcgccaagt
    22681 cgatcgggat ctacgcggca gcaagggagc gtcccgggtt cctgaccgtg aagaacctgc
    22741 tcgttggggc ggtagccatc ggcttcgggt tcctcgtcgg ggaaaaactt ctcctcttcg
    22801 ccacgctcgc ccagatcacc gagtcgatct tcgggagcgt tctcttcctc tccctccagg
    22861 tgctctggat gccgctcctc ctccacatcg cgggagtgct gatcaccggc ggcttcctcc
    22921 tgctctgggg gcgacgtggc tacggaccgg ggcttgtcgt ggcgagcgtg gtgcacagcc
    22981 tctataacct ccacttcctc acgggagtgc tcctatgaac gcgcgccata tcggtcttgt
    23041 cgcgaagaaa gaactctcag ggctctcgtc ggagaagacg atcgtctttg ccgtcctcct
    23101 gcaggtcttc atcgcaatgt tctcctcgtt tctgatggtg gggctcaccg ccatgtacga
    23161 cccggaggcg atagagagct actcgacggt cacctacggc gtcggctacg ccggagcgga
    23221 ctcagccctc atcgacgaat tttcaaagcg aaacgacctc gtcctccacc ggatggacct
    23281 cggccaggcg cttacggcgc tccgcgatcg aaaggttgcg gcggtggtct atgtcccgga
    23341 tacaccgccg gatgcggaag gaccggtcac aatcacgctc tacctcatcc agaacgacct
    23401 gcagtcgagc gtcatcaacg tcaaggtaaa agaggtcctc cagagttacg agaaggagct
    23461 ccgggaggtc cgggccgacc ggatgacctc cttccccatc gggctgaact tccccgagtc
    23521 cgggggcggc gagaacttct tcgagttcgt ctacggcctc ctcatcccgc tgctcgtcct
    23581 cctcccggca atcgtctcgg cggccctgat catcgacctc atcaccgagg agtaccagcg
    23641 ccagaccctt gaaacgctca tgtcgacgcc ggtcacgttc gcggagatgg tctgggggaa
    23701 gatcgccgcc tgtgaggttc tggtcccggt ccaggcagga gtgtggctcc tcctcctcgg
    23761 ggtaaacggc atcgcgatcg acaacgccgc cgcgatcctc ctccacgcct cggcgggcgg
    23821 gctcttcctc atcctgctcg gtgctctcgt cgccctctat taccgcgagc ggacgagcgc
    23881 ccagttcata ttctcgacgg cgctcgtcgt ggtcttcctc ttcgtcatgg ccgtccccta
    23941 caacccctta aacctcatgg tcaggctgtc ggtggacacc gccggtcccg tccactggct
    24001 gatccttctt ctggtcgcgg gcgcgacggc actcctcgga tgggccacga cggcctacgc
    24061 ccggcggatc gcggaaatca acccccaaac ccggtgagcg cgggaacccg gtcgtgtacg
    24121 gcccggaccg gtgagctgga ggggttcaaa gatagccctt atttcgccgg gatctatccc
    24181 gggtaaaata tatccctaag gtcatgcaat tcagccccat cgatgactca ccttcgctac
    24241 ctcaatacta cctgtgcagt ctgcggcgag tcctgccggt tcaccatccc cgaggccgca
    24301 ggcagcgtcg ggccgcggga cctcgatacc cgtcccgccg aacccctgcg gtccaccatc
    24361 tacgcatggg tgaaacggtg cccgtcctgc ggctactgtg cccccgaccc gggcaaggct
    24421 ccgaaggggg cggccgacgt ggtgaaactc ccgcgctacc gctggcagct tgatgcccgg
    24481 aagttcccga agatagcgaa caccttcctc tgctggtcga tcatccagga ggatcttgcc
    24541 gtccccgctc aggcggcatg gggatccctc cacgccgcgt ggatctgcga cgacgaggga
    24601 gaggatgtgc ccgcccggat ctgccggaaa cgtgccgccg acctccttcg gcgggcgtgg
    24661 gagcagggcg agcgcctgtc tcctctggca gggacggacg aggcgctcct ggcggacctg
    24721 ctccgccggg cgggcaggct cagggaggcc cggggcgttg tcgcggagac actggagcag
    24781 aacccgcagc ccatcatcgc cgacattatg cggctccagg cccgcctgat cgccgggtcg
    24841 gaccggagcg cccacaccgt cgccgaggcc cggcggttca ggcagcctgc accgctctga
    24901 ccggtggtcc ggcggagcct aaccatttgt atactgcttt gccgatactc tatcatggat
    24961 atcacgatcc tctctcccgg catctacacc tacggcgcca tgctgatcgg cggtgtcctc
    25021 cgcgacgc
//
LOCUS       DAJB01000006            5302 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_114108, whole genome shotgun sequence.
ACCESSION   DAJB01000006 DAJB01000000
VERSION     DAJB01000006.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_114108"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1229 a         1549 c         1351 g         1173 t
ORIGIN      
        1 gcccgcccat aatctacata gaaccgtcct tccgtatgca gcaggtttgt gctcccccaa
       61 gaatgccatc cgttctcaca ccaaacgtcg aatatatcaa catactttaa ctcagggccg
      121 gatccctcat caggcagtgt caactcaatc cgatataatg ccataccggc ctcaacatca
      181 ttcgtttcca gggacaactc ctcgaaacgt atttccccca ggcatacagg cgtcaacagg
      241 tattgtcgtg tactgccacc accggacact atttccagaa tcgtatcccc ctggtctatg
      301 agaacaacgg tataatctcc gtctttagcg atgtctacaa aacttttacc gaccgaaatc
      361 tcctcaaggc tgttgaagga ctgctcaacc cctatccgat tcgatgcagg aaatacgatt
      421 tcatttgatt gaggagaaat attcaaaagt gtgtaggggc tattcgtagg gatttttccg
      481 atttttgtcg cttctgatga agtaagatag actacaagtg tcaaacatga actcagtatg
      541 agcaggatag caataacttc aaagaggagt aaaatcacgt tgctttgctt taccatgacc
      601 catcacactc ttcttcagat gcatctctcg tagatcctca tgagccgtat tcaaagtggg
      661 tttttctacc gcttctctag tgcctcaagc cgctcttcaa tccgggtcat ggacatctgc
      721 atcgaacgta gcaactcaac gttttccgca gggcctgaag ggggtttttg taaaacgtcc
      781 agatagcgtc tttctctact gtcagcccac gatttaagat cacttgcgag actctcaata
      841 aacattagtg tgatgataac tccccagacc aaccataagc cggtaacagc actgataatg
      901 gtcactccta tcgctgcgag aacgtacaag gcatttttac tcttttcttc catgatcacc
      961 aaatcctttg tttctagtat gcctaatcaa aacagaatta ttatgatgta caaccatcac
     1021 cgattacaga cagccactcg ttttgagaac caccatcgtt tgtggtaagc catgcgttac
     1081 aactgaccca tgcatccatc tgtgcgcggt tatagagaat ccctaccttt aagtagccat
     1141 tcacctgacc agaggtcgta cccgcaccag acgatgatga gctaaaatca catttactcc
     1201 agagagtgta aacactccat gtggttctat caacgattcc agaaacgctt tgtccataat
     1261 ttacgtagaa atcacccttt gttacaagtc tggccctaac gacaagatta gtcttatact
     1321 catcagtccg actaactgtc accatatact gatcggccat gacaatggct gggtctgggg
     1381 acctgatcgg gacagaataa agtcgaatat tcgtttcaac tgtgaaattg cactcttcag
     1441 ggttctgttc tccaagggac acttgcttcg gactgtgtga cgctcttttc tcccctaata
     1501 actccggggc aattttatac actgttacag attctccatc ataaaccgct tcgagcacaa
     1561 ggtctccgcg gctgatgaga aggatctggt actgctccag atcgtagaga ccgataacag
     1621 cgtcagtgtc atcattcagt tctgcttttg ccattatccc atcaatgaga tcttggcact
     1681 cgttgtacga cttgagtggg ggtgcgtcat cagagccctc gggtgcatca ataccgaatt
     1741 ccttcagtaa atttgtctca gaaccagatt cataagagac aacaccggtc tcagggatgg
     1801 aacatgtggc acatgcttgg tcctgaccgc cgaggatatc tttctcagga gtaatgtcct
     1861 gtgcagctgc taaagacatt actgccgata ccatgagaag cagtcccagt aagactactc
     1921 cggttctttc tagtttcata ggccgttttc ctccaattac tgcatctctg gaggaacgga
     1981 accacaatgg ctatctctaa tgaatgccaa cattccgttg cctccgggta cgcaggacgt
     2041 tcgcccatcc ggttcgcttc tggccgggtg aacccccgcg ctgttacaga cgccgcacgc
     2101 ggcacctact acggttctga gccgttagaa tatataaaac ccgcagcaat ccgtcggttt
     2161 tttccgaaca atacaaaaac gtttctatag gacgaccacc cacaggtggg ccgctcaccc
     2221 ggcgggacgt atcccgggcg agtccgtttc gtaaggacta tacgtgcaaa accaggcttt
     2281 ccgggagccg tgccgcacgc cgccggcagc accaccgcca cgggatcaga tcgccctcac
     2341 catccggaac cggatcgcaa ggtttaaggt ttgttcggcc agaaccgaac gacgacagcg
     2401 ggctttataa gattccccct cgaattcggg tttagttccg ggtgcaggaa cgcccgggcg
     2461 caccttgagg gatactcatg aaacaatggc tattcatgct ggcagcactc ttcctgctcg
     2521 cggctctgcc tgctgccgcc agcgccgcac cactcacgca caactatgac tgttcggaca
     2581 cgacgccgac ggcttacgat gcacgggtgg atcaggcagc gatgtactac tccagctcgt
     2641 cgtccacgaa tgcacacgca tcgacggctt acagtcagtt caggtcggat gctgtattct
     2701 tcttcaacgg ccacggcctc tactacgacg actcgcataa aggcggtggg ataaaattcg
     2761 cctcggaatc ctggatactt gccgacaccg atggtcacta cccaggaaac gaccagtatt
     2821 acataactga ttatggaacc gacatcaggg acgtcctgct cgcagtctac cttgcctgct
     2881 attcgtccca ctacaatccc tacaacgggg atctcctcca gcagaccatg aacaaaggcg
     2941 cggacatctg cctggggttc gacggcgagg tagaggtcga aaacggaaag tgctggtccg
     3001 ggagcttctg ggaccgcgta cgaaacgggg agaccattgc aagcgccgca agcaacgcaa
     3061 aggacgactg ctacttgcac tggccgatcg gctactgggg agtggacacc tacgagatct
     3121 ccggcaatta caacagttat ctcactcccg ctcgttacgg agtctattaa cgaggtgatt
     3181 caaccatgaa atggatacca gttactgcaa tcatatgcct catcatcgct gtctcgatca
     3241 cgtcagttgc cggcaccggg accgcgattg gagtagatac tgcgaaaaaa gaagtacagg
     3301 actttctcaa cgcacaggat gcggccattg agtaccagaa gaccgagcac ctgaatcttg
     3361 gaaattacta cgtcttcggc accggagagg gacagattta cgtcaatgac cagaccgggg
     3421 cggtcgaacg cgccgcattc ggttctgcac ggaaagactc ccacgagatc cggctggatc
     3481 gggccgcagc tgaagcagag gcaagggcct acgcagggga gaagtacggc ggttttgcgg
     3541 agaagaacat gcggcttatc gagtcaaacc tgatctctca cggcgatgcg ggcagcgagt
     3601 attcgtacat ctggagagaa gagatcagcg gtgtcctcac gccgaacacg gtcgtcgtga
     3661 accttaaccc gagcaccggc gagatcgtct cgtacctcgg gatccagcgg gaggtccggt
     3721 gctcgcttga gccggcactc tcccgggacg aggcgctgaa ggccgcagcc gggcagttcc
     3781 cgggaatccg ggtgaccagt gcaacggccg acctctccgt tgagtacacc cgaccggacg
     3841 cacagacgct gacgtgggtg atcacgatga gaggcgagcc agaggaccac gttctccagg
     3901 gagggcttgt cgttatcgac gcacagaccg gagaagtgct gatggtgagt ccatacctct
     3961 aaatcacctc ttttcctaac gtgatacaat gcactcgaaa atgtggatgt gggttggcat
     4021 tggcgtcgtt ctcgttcttg ctgcggcaaa catcgccata tttgcggcct tccccccggg
     4081 acagcagcat gtgcaggaac cggtcaagcg cgtgaactgg atactgccgt tcgggccggg
     4141 gttcgaatac cgcagttcct acgagagccc ggtgatcgta tacgacagga tgaacgagac
     4201 ctggtatctc aagaacgcta ccgcctggaa ccggacaatc ttcctcggtg accgctacag
     4261 cctcgatccg gcgcatccgg agggtatccg ttacaccccc gacatcgcaa tcaagggcga
     4321 caagatcagc ctcgacttct tcgtccggaa ccttgcgggc gaagactgcg cccatgccga
     4381 cctcaccgtg acgatggaaa ccgcgagcct gaacgccacc accgggtcgc cggaggggga
     4441 cattttcccc ggcgccatga ccacgctgcc cgtcgacgac ctcgccgccg gggagtggcg
     4501 cgaagtgcgg gtgacggcgg acctcccgcg gcccgggccg gaggaggtcc tctccctgac
     4561 gatcggtctt gccgccccgt ataccctcac gaccccgaac ggcacgccga tcatcttcga
     4621 tctccgggag atgagcagcg tcatctcggg ttccacccct accggcgaca gcccccgtct
     4681 tccccgggcg tcggcgacgt tcgtcgtagc ggggaaagat gcacctgaac ctgttctgcc
     4741 gccgaccctc cgggcagtgt gacgggattc gccgaatacc ctctttttcg gccttaccgg
     4801 aacccagagg gttccggaat gcttttctct cctggcggaa gaatacctga tcgtttcggg
     4861 ccagactatg gaagaaacta cgctcgatcg ggacgacatc actgctctct cctccgacgt
     4921 ccgggtcatg atcctcaaag cgctcgattc ccggccgatg acggtcagcg agctcgccga
     4981 tcggctcggt cttgcaaagt cgaccgttca ccagcacctt gccgttctcg ccgacgcgga
     5041 tctcgtggcc cacgagggtg cccggaaatg gcgggactac accctgacga ggagagcccg
     5101 gcggatcctc catcccggga gggatcaccg gatcaccatc atcctcagca cgtccctggt
     5161 tgccgtgacg gccggagcgc tctgcgtggc ctcatatctc cgcggtttcg tagtccaggg
     5221 gggaagcgtc gtccgggaac ccctcctcct ctacgcgggc gaggcgctcc tcggtgtgac
     5281 ggtcgtgctc tggtgccttg cg
//
LOCUS       DAJB01000007            7045 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_69070, whole genome shotgun sequence.
ACCESSION   DAJB01000007 DAJB01000000
VERSION     DAJB01000007.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_69070"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1438 a         2215 c         2138 g         1252 t
ORIGIN      
        1 cgcagtcgga actcgccggc ctcgacctcg aggtgacggt gctcacgctg ccccgaccgc
       61 tcgagtgccc gccacaagag cgccccggct gcgtcgaggt cgggaagcac ggcctgatcg
      121 tcagcggttg cgggaccgga ggcctcctcc tcccgcaggt cccgaccgag tacggctgga
      181 ccagcagcga gttcctcgac cagacctgcg tcaaggccgg gctttcgccc gggtgctgga
      241 ggcgcgacga tgtcgccgta cagactttcg aggggcagat attcgaagaa aaggcggttt
      301 aacccgaatg gcaattactt ttgaggtcat acacaaagat atcgcaggaa gggtcggcag
      361 gctcagggtg aacgacaaaa cggtccggac acctgcgctc cttccggtcg tcaaccccca
      421 ccttcccctg gtaacccccc gcgagatgca ggagatgggc gtcgaggccc tgatcacgaa
      481 cgcctacatc ttcaggcgga gcacggagta tcacgaccgg gcgcttgcgg aggggcttca
      541 cgcggtcctc gacttcgacg gcgtcatcat gaccgactcg ggatcgtttc agctctccgt
      601 ctacggggag gtcgaggtga gcaaccggga cacgcttgag ttccagcagg cgatcgggag
      661 cgatatcgtc gtccccctgg acatccccac tccccccgac gcaggccgcg agagggcgga
      721 gcgggaactc gcggtcacca tggaccggat ccgggaggct caacagctct tccccgacgc
      781 gaacctggcg gcaccggtgc agggcggcat cttcaccgac ctccgcgagg aggccggaca
      841 ggccgtctcg gacctcaact tcaccttcgc tcccatcggc gccgtcgtgc cgctgatgga
      901 gagttaccgc taccgggacc tggtgcaggt tgtccttgcg gccaaacgcg gcctctcccc
      961 ggcgactgcc atccacctct tcggtgcagg ccacccgtcg atgttcgccc tcgccgtcgc
     1021 gatgggctgc gacctcttcg actcagccgc atacgccctc tttgcccggg aggggcgcta
     1081 catcaccccg cacgggagtt tcaagatcga cgaacttgcg gaacttccct gcccctgccg
     1141 ggtctgccgc tcgatgaccg ccgacgaact ccggcagtcc aaagaccggg aacggctgct
     1201 cgccctccac aacctctacg tcagcctcgc ggagatcgcc cgcatccggc aggcgatcca
     1261 ggacgggacc ctctgggaac tggtcgacga gcggtgccgg agccacccgc gtctgctcga
     1321 cggctaccgg gaactcctcg cccacgcggc cgatctcgag cgcgacgatg ccgtctccaa
     1381 acgccggttc ttctaccggg gctcggagag ctgccggcgg acagaggtgc tccggttcca
     1441 ggaggtcatc tcccggattc ctatcggtgc ccgggtgctg gtctcgttcg acggccggga
     1501 gttccccggg tttgataccg tgctgaactt caagcctccc ttcgggccgt accccacaga
     1561 acttgctgag acgttcccgg taggccagag cgagatcccc gaatgggacg acgacatggt
     1621 tcggtcggga tgcgccggca tcagggctct gatggaggcg caccccaaca gtcggttcac
     1681 cgtccagtgc ggtgaagagt ggacgcgcct cgtcctcgaa gagatccccg atgcggaggt
     1741 gtacgatgag caggtatgat gcgggaaagc gcgatggcct cgcacggata ggaacctatg
     1801 agcacgaaga gaggagcgta tcgctcccgg ccgccctcga tacggatacc ctcttcccgg
     1861 cacttcgcga gcggcctctc tcgaacgtcc cgctctctgc ggatccggca ttcgtcagca
     1921 actactttag ccccggggag gggcagccgg tgacgatcca cccgctcgcc acgcaggcag
     1981 cagagtcggg cgactgcgcc ctggtcgcaa actggcatac gacactcaaa aacccccgga
     2041 gctacgcggc gtggcttgcc accttaaaag agagcgtccc gccggacacc gcctggtacg
     2101 ccccggcctc ggcgctcccc tcgaccgcct gcctcctcat atactcgggc ttcgacctct
     2161 tcgactaccg ggccgtcgac ctcgcctctg cacggaaggc cttctgcctg ccggaaggcg
     2221 agttccccgc ctcgctcatg gagacggggg tctgcgggtg cgagggatgc cgggacggcg
     2281 acctcaagcg gcacaaccgc ctggcgctgg accgcgagat cgcccttgtg cggcactaca
     2341 tcgaggcagg aaggctccgg gaactgatgg agtcccggtg ccgtgcggac gccgcacagg
     2401 taggcatcct ccggttcctc gaccggaact atgctcttat ggagcgcttc ctcccggtgg
     2461 tgcgggcggt gccgatgcgg gcaaactccg ccgagtcgca gaaccgggcg gaaatccgcc
     2521 ggttcgccga ccgggtgatc gagcggttcg tcccgacccg gaccgacgtc gcggtcctcc
     2581 tcccctgctc ggcgaggaag ccttactcgc tctcccggag ccaccggctc ttcgtgaata
     2641 cggtagaccg gcgggcgcac gaactgatcg tcacctcgcc gctcggcctc gtgccgcggg
     2701 aactggagcg ggtctatccg gcgggccact acgacgtgcc ggtgaccggc tactgggacc
     2761 gcgaggagtg cgccttcatc gccgacatcc tcgcccgcta cttcgccgcg cacccctacc
     2821 gccgggtagt cgcgcacctt gagggcggcg cgctcaccgt tgccgagatg gcggcagagg
     2881 cgtgcgggtt cgacctcgag gtgacctgct ccgggcaccc gacgtcgccg ggctcgctcc
     2941 gcgccctgaa tgacgctctt gcaggcgagc ggcgggtgca gaccgacacc atccggggca
     3001 cggtctcctg gcagttcgcg acggatatca gcacgaaggg actccagatc cggggcagga
     3061 gcatgcagat ggcggtgctc cgcgggaagc agcaactctt cagcatcgac ccggggaccg
     3121 ggctcttccg tccgacattc gatgggtggc ggatgatacc cgagggctac cgggtcaaga
     3181 tcgacgcgtt cgtgccgcag ggcgatgtcc tcgccccggg gatcacggac tgcgaccccc
     3241 ggatacggga gggcgacgag gtgctggtgg aaggtccgct cgcgatcgcc accgggcggg
     3301 cgatgatggg cgcggacgag atgctccggt cgaagcgcgg catcgcggtc agggtaagga
     3361 agacgcagaa gttttcaggg tgatgatggc ctgcgccaaa cgaggcttac ctctgccccg
     3421 ggtggtccca ggtgggtgaa ggacgaccat ggcgttaccc gactttgcca ctgaagagca
     3481 gcgaacactt caggaaagcc tacctgcagc cggccatctg ggccaaccgg gtcgagatgg
     3541 cccttcccga caggcctcgc agcagcaata cggcacggag tcagcggttc acgataggat
     3601 cgtccccctc cggaaatatc gtgatatttc gcttgtttag aatcagatcg gatatctgct
     3661 tctcaaactc gaccagaaca ccggacgctc aaatagcaac tgtaattcat cagaacgtgt
     3721 aataaacgtt tgaatgagtc cgcgggcaaa aaagatcagc aatccaatct cctccggtgg
     3781 cggcggagtt atttttgaaa cacgtgtcca agctgcgttt gctatattaa tactgaccgg
     3841 aggacatgcg tcatgtttgc ccgcgtggcc aataaagaaa atcaaacttc aaggaaagca
     3901 tttagactat aatacagacg acctggttat ctttacgaaa gaatcggtaa gcggtattga
     3961 agccagggta gaggctcgaa gttacctccg tttagcaggc tcgctaagag agttggcgga
     4021 ctcgtacaag cgtgatgctg gatgggaatc aagaggaggc ttattagatg attaatcagg
     4081 tgaccggcaa catggccttt cccaaagcga aaaagcccga gtggatacct cacgacgagg
     4141 cactcctgtg atccctgata ccctccaggc cctactcgcc accggtgaag acagccgccg
     4201 ccagttcaag cgcaacatca ccaataccga tgcccttgct gcagagatgg cggcatttgc
     4261 caacgccgaa gggggcacgc tctttctcgg tgtggccgat gacagttcca taccgggtct
     4321 gtcacgcaca gaggtaagcc ggatcaatca gctcatcagc aacgcggcct cgcagcatgt
     4381 gaagagcccg ttgacggtgc gaaccgagaa tatccaggta gacgaagacc gtattgttat
     4441 tgtactcact gttccgaagg ggcaggacaa gccttacttc gatagaaacg gcgtgatctg
     4501 gttgaaaaac ggagcggaca aacgacgggt gaactccagg gaagaacttc gccgcctttt
     4561 ccagagtgtc gatatcttcc atgccgacga gttgccgacc aaagcaggca tcgacaaact
     4621 agacaggctg cgtttccggg atttcctgcg gaatacctat aaccaaccct acccagattc
     4681 accagagaaa ctgataacaa ttctgcgaaa catgaatctg gctacagatg acggcatgct
     4741 gaacctggcc ggggtgctcc tttttgctga gaagcccgat tggatcaaac cgcagttcat
     4801 cgtcaaagcc atccgctatc ccggcaatgc catccatgta agcgagtatc tcgacaccga
     4861 ggatttttcc gggacatttc ctgagatctt cgagggcgct atggccttta tcatgcgcaa
     4921 cctcaggaaa attcaagcag gaaaaggagt taacactccg ggggtcccgg aaatcccacc
     4981 actggtcttt gaggaactgc tggtcaacgc tctggtgcac cgggactatc tgatcagcgc
     5041 accgatccgg ctgttcatct tcgataaccg tatcgagatc atcagccccg gccacctccc
     5101 aaacaatctg acggtggaaa agatccgggc tggtaactca aatatcagga atcctatcct
     5161 ggcatcgtac gtcgcaaagg ggctgttgcc ctaccgcggc ctcggctccg gcatcaagcg
     5221 tgcccttgaa gactggcccg acatcgacct caccgatgac cgggacggct gcctcttcac
     5281 cgccacggtc caccggaaag aggagaaaag ttcggagaaa agttcgnntt cggagaaaag
     5341 ttcggagaaa attcttgttc tcctgaagtc cgaacccgga ctggcagcca gagaggtcgc
     5401 agaaaggctg gagatcacac caagggcggt tgagaagcag atcgccaacc ttcgtaaaga
     5461 tggccggctc cgccgcatcg gccctgcccg gggcggccgc tgggaggtgc tggactgacc
     5521 ttcaccgaac cagaatccgc cacgcgaacc ccaaccagaa aaagaagggc aggataccct
     5581 gccataaaac caaaaaccct tacttcctcg ggacgagttt cactgccgtc ccgtaggcga
     5641 ggagttccgc cgcggccccc atcgtctgcg acgtggtgaa ccggatgttc accaccgcgt
     5701 cggccccgag gtcgtgcgcg gcgttgacca tccggttgta tgcctcggtc cgggcatcgg
     5761 caagcatcga ggtatactcc tcgagttcgc cgccgacgag gctctttagc cccgccatga
     5821 catcctttcc cacattcttc gtccgtacgg tgttgccaaa gaccacgccg aggacttctc
     5881 ctacggcgta gcccggcact tctgcagtcg tcgtcagtat cattcttcgt tcactccctg
     5941 attgtttcga tggtctcctc gcgcccccgc cacacgatca gcgcgaggcc gatcacgatg
     6001 agcgggacgg cgtagatgag gaggaacgga aaccttatgc ctatggccgc gatcactgca
     6061 ccgatgacgg tcagggcaat cccccagcgt acctggctcc gcatacatag ccatgcgacg
     6121 ggtgcgggga taaccattcg catttttgac ccggttccgg acgcgcctcg cgattccccg
     6181 ggggaatcgt ccggcactga ccggcggtgg tctgcgggga atagctgcag taaagaggct
     6241 ggagccacaa tatgtacgaa atgttcagtt gcatcttgac gctaagatcg accaacatca
     6301 tcaggagtga agcagttgta taaggtggaa tcagcgacta aactcgccga ccgggtttat
     6361 gaatactgga tacgcgcccc gcaggtggca gagcacgcac gggcaggtca gtttatcatc
     6421 ctgcgcctgc acgaagcggg cgagcgggta ccgctcacca tctccgccgt caggggagac
     6481 accgtccggg tgatctttat ggccgtcggc aagacgaccc aggaactcgc gacgctccat
     6541 gcaggcgaca gcataaagga cgtcgtggga ccgctcggga agccgagcga gatcgagaac
     6601 tacggcacct gctgcgtcat cggcggcggc gttgggatcg cgagcacgcc cctcatcgca
     6661 aaggaactaa aaggtgcagg caaccacgtc atcgggatca tcggggcgcg taatgccgat
     6721 ctcctcattc tcgaggacga gatgcgggag gtctgcgacg agttctacat cacgaccgac
     6781 gatgggagca agggcgtcca cgggtttgca gccgacgtcc tgaagaagct gctcgaggag
     6841 aggaagatcg accgggtctg gatcatcggc cccgccatca tgatgaaggt cacctccggc
     6901 gtgacggtgc cctacggcgt gaagacctac gtcagcctca acccgatcat ggtcgacggg
     6961 acggggatgt gcggctcctg ccgggtgacc gtcggcgggg agacgaagtt cgcctgcgtc
     7021 gacggccccg agttcgacgc tcacc
//
LOCUS       DAJB01000008            4652 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_92526, whole genome shotgun sequence.
ACCESSION   DAJB01000008 DAJB01000000
VERSION     DAJB01000008.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_92526"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          782 a         1456 c         1537 g          875 t
ORIGIN      
        1 cccctcccct gtccccaccc cccagtcgcg atagccacca cggtccagta gatcgggttt
       61 ttcacaagtt tttactgttc tgaggcgcaa tcgaagagcc cgatctgcaa ccggctcccg
      121 ttttcccctc tcgtatatgg taagatgacg ggaactatac ctgaatgccg gcaggaggaa
      181 caactccagc cttacaccct ctccgtcccg tccctctctc cccccgaaca cccgatcggc
      241 tcgatccggg ttcccggcag gattaccagg ttgtacgcgt tgtgatcctt gttgaaacgg
      301 acgagggccc cgtaaaaccg gacgatatcc ccataggcaa tatcgcggag cgcgtcgcgg
      361 ttcggacccc ggagggtcgc cgggaccacg acataggccg ggtttgcggg tgtcccgtcg
      421 cagaccttgt agactttcgt ccggttgtca ccgaggttcc cggacatcag cgcggcgacc
      481 acgttgatct tccgatctac gtgctggtgg aggtgggaga ggcgggcgac ctttgctccc
      541 ggcgggaccc ggtaggcctg ctgcgtcttc ttatccttcc gcaggagtgc ggaggagtat
      601 ttgatatcga cgttgatgaa ccggtagcca tccgcccccc ctgcgagacg ctccatcaac
      661 cgggtcgggt ggatattggt cttctgctcg aaggtccagc accgcacggg gttgcatgcg
      721 gtctcccaga tgaacgagca ggggctgtgg atggtcaggc cccggttcgc gatcgcgcgc
      781 acggtctggc gcatggcggt cgagttggcg aggtctgccg gctccacgac gacgatcgtc
      841 ccatcgggcg cgaggcggtc ggcaagacgg gcgacgaggt cggcacgccc ctcaagatcc
      901 aggtggcgga gttcgttcaa gacgttcgaa aagaccatca aatctatcct gtcgggcagg
      961 tctctggcgg cgagtgttcc gatatctcca cggatgggct tatggacctg caccatgttt
     1021 ccctgcgctt cggccgctgc cgggacgagg gcgttgtaag cctcgaaatg ctcttcggag
     1081 cgctctacgg cgtagatctc ggccgtgccg ctcccgatcc gtccgaggag gtcgatgacg
     1141 gcgagcggca cgacccccgg tccggtgccc acatccagga tgcgcatgtg cggtttcgcg
     1201 agtccctggt tgatgagctg ccagaagatg tgctcgaact gcaccaggta gacgggggcg
     1261 tggtaggcca ggtagccgag gaccgggtag gccttccggt aactcacctg ccgctctttc
     1321 cagtactggc tcttctggac gaggatggca ttgcgcatcc ggtcgagcac ggccgggtcg
     1381 tcccaggact tggcggtctt cctcccgacg taggcctcaa ccagctgctg aatgtagtcc
     1441 gggatctccg ggttccggaa gaacgcgagt tgccgcagcc gcccgatatc gctgaccgtg
     1501 aacggcctgt ctgctgagag gcggctgatc acgtaatcat cgccggcaga cctgagggtg
     1561 aggccgaggc cgtcgaggtg cgggcggatc tcaccggcga ggcgctcgag cgactgcctt
     1621 tcacccgggt cttcctgtag ttccgagagc cggaactcgg gctttgcgcc tgcaagacgc
     1681 ttcagtgctg cagctgtatc ccgctcggtc atggcgtacc agctcggcgg ggtggttaat
     1741 acaggttccg ggcggcgctc cccgggatga tctcaacgcc ggggtacggc tgttcctgtt
     1801 gcggcgtcct gctgtgcgta aaataggaga tggcgggtcc ggagagcctc actcagaggg
     1861 cgtgccggca cccttctccc gcccgagctg gaaggctctg gcgttgactt cgacggtctt
     1921 tctggggacg caccgccgga ccgcctcttc gagggtctcg ggccggaggg ggatatcggt
     1981 ggacgcagcc ccgagcatca cgatattctg cgagaggatg ctccctgcct cctctgcgag
     2041 gtccgcggcg tcgatgcagg tgacgtcgag atcggagagc gcggcaagga tgctctcctt
     2101 gtcgggcacg tccagatcct gctggtagac cgatgtgggg acgaccaggt gccggttcac
     2161 cacgagtttc cccccggccg gcaggtagtg gcggtagcgg agggcctcga ggaggtcgaa
     2221 ggcgatcagg aggtcggccg tccccggcac gatcagggag ccgaacttcc cgtcgatgcg
     2281 gatatggctc tccaccgacc cgccgcgctg cgccatgccg tgagtctcgg cgctccggac
     2341 ggtcctgttc tcgatgatgc acgcctcgcc gaggacattc gaggcgagca cggtcccctg
     2401 gccgccgatc ccgacgatca ggatatcaaa cgaaggtttc atcgccgtcc ctccataccg
     2461 atcgcacctg acgggcatat gtccgcgcag acaccgcacc cggcgcagag ttcggtgatc
     2521 gtagcattct ctccgataaa tgcaatcgcc gggcagccga atgaccggca ggcgccgcag
     2581 cccacgcacc gctccgggtc gactatgtag gccttgcggc ggataccgct ccgccgtgcg
     2641 gtgatgacgc agggctgctt cgcgatgacc acacggacgc ctttgcgctc ctttgcctgg
     2701 cggaaggtgt cgaggagcac cgggaggtcg taggggtcga ccgtctcgac ccaggagacc
     2761 ccgcacgacc ggcagatcgc ctcgagcgat atcggggtgc tctcctcgcc cgtggctgtc
     2821 aggccggtgt tggggtttgg ctggtggccg gtcatggcgg tgatccggtt gtccaggatg
     2881 acgacgacca tatcggcgcc gttatacacg gcgtttaaga gccccggcat ccccgtgtgc
     2941 aggaacgtcg agtcgccgat ggtagagacc accggacgct cctcccccga ccgggagatg
     3001 ccgctcccga cggtgatcga ggcacccatg cagatagtcg tatccaccgc cccgagctgg
     3061 agcccgaggg tgtagcagcc gatgtcgctt gggaagatgc cgtcgcggaa gaccttccgg
     3121 atcgcgtaga acgttggccg gtgcatgcac ccggcgcaga ggatcggcgg gcggggcggc
     3181 accccctgga ccgggacagc cgccgggaag actaccgtgg agccgatgcc cgccttatcg
     3241 agggcggcgg cgacggttgc cggggtgaac tccccctcgt acgggaccgt gccggtcatc
     3301 ttaccgagga cctccgtctt cgtcgccacc tggagtaccg cctcctcgac taccggggcg
     3361 agctcttcga ggacgaggac tttctcatgc cggtcgacga aggctctgag ccacccctcg
     3421 tcgatcgggt aggcgccgat gatcgcgagc gagacgtcgc ccccgagcac ctcctggacg
     3481 taggcggcgg cggcaccgct cgcaacgacg gcggtgctgc cccggatggt gtgccggttg
     3541 tagccgagtt cgaccagccg ttctttcagc gccggctgtt ttccgttcag gattttatgc
     3601 agcacccggg tgtgcgcggg gatggccaca tactgcttcg ggtcgcggtg gaaggcggcg
     3661 gtccggtgaa cggttccgac ctctccgagg tcgacgtcac ccttagagtg gcagatccgg
     3721 gtggtgggcc ggaagatcac cgggagaccg aactcctcgg agagggcgaa ggcgtcccgc
     3781 agcatatcgt gcgcctcctg gatcgatgcc gggtccaggc atggaagccg ggcaaagtgc
     3841 gcgtaacgcc gggtgtcctg ctcgttctgg gaactgtgtg caaaggggtc gtctgcggaa
     3901 aggacgacga acccgccggt cactccggtg tatgcgctcg tcatcagggg atcggccgcc
     3961 acgttcagcc cgacgtgctt catggtgcag agtgcgcgca ggccgcacca ggcggcggca
     4021 agcgcgttct cgaaggcgac cttctcgttc gtggaccact ccacggtgaa ggggcgctcc
     4081 tcctgcaccc ggaggatgtc aatcacttca gaagatggcg tcccggggta tccgctggca
     4141 aaatcgatgt ttgcctcaag gcaagcgtgc gcaatgccct cgttcccgag taggtatcgt
     4201 actgccattg ttccactcaa ctgtacttct attgtcggtt tttaacatag cgctttggat
     4261 gcctctacac aacaattaag tgctaggatg ccagatgttg cagggtaaac ccaccggggc
     4321 ccgtagcata gccggtggtg cacccggctg ataaccggga ggtcgtgcgt tcgaatcgca
     4381 ccgggcccat atcctttgta taacctttct ctggtgagcc ccgcgtctcg gcaagggttg
     4441 cgggtacgnn cctgcaaggg cggcgtccgt cgtggggaga ccgccatgcg caccgaaacg
     4501 gacgaagcaa ggcgacgaca gtcgcgaagt cgatgctcgc ctcgatgcgg tcatatgcga
     4561 cggcttcata tacgacgggc aaggagaccc ccggtttcaa ccgggggagg aattgcccgc
     4621 atcaggcccc gtacacgttc gccccgcggg ag
//
LOCUS       DAJB01000009           11011 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_10165, whole genome shotgun sequence.
ACCESSION   DAJB01000009 DAJB01000000
VERSION     DAJB01000009.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_10165"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    10828..10889
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1961 a         3489 c         3391 g         2106 t
ORIGIN      
        1 cccccttcac ccccacaaga taaaacctaa ataaggttag ggccatacgt tgaagcggtc
       61 aaaatctggc ctttttgccc tggggtgcaa acacgccagg agtggcacat accgccacct
      121 ctgctctatc tccggcctat cggcgcatac gtcgtccgga cagttcgtga gatcccggaa
      181 ccccacttag ccgggctctg aatgccattt agccgcggtc tcgcggcaaa aactacaagg
      241 tgatatgtat ggtttataag atgagaagcg cactcgcact gacaggtatc gtcctggtgc
      301 tctgcctctt ctccgccggt tgcaccgaca cacaggcaac cgggcccaaa aaggagatca
      361 gcatcgggta cgtcacctgg gactgcgcca tcgccagcac caacgtgatg aagcaggtct
      421 ttgaagaggc aggctatgac gtcaccctgg tcgcagtcga cgcaggaccg ctcttccagg
      481 cgctcgccaa cggcgacgtc gacttcacga cgaccggatg gctcccgcac acccatgaaa
      541 gttactggga gcagtacggc gaccgtatcg actacgtcaa tgagaacatc cccggagcgg
      601 cacgcatcgg actcgtcgtc cccacctacg tgaccatcga ctcgatcgag gagatgaacg
      661 gcgtcgccga tcagttcggc ggcaggatcg tcggcatcga acccggtgcg ggcatcatgg
      721 cccggaccga gcaggccatc gatgagtatg gtctcaacta cgagctggtt gccagcagca
      781 gtgccggcat ggcggcggag ctgggctcct ccatcaacga tgaggagtgg gtcgcggtga
      841 ccgggtggtc gccgcactgg aagttcggcc gctacgacct caagttcctt gacgacccga
      901 agggtgtcta cggcggggcc gaggacatcg tgacccttgc acggcagggt ctcgcgaccg
      961 acgaccccga ggcctacggc atcctgaccc ggttcgagtg gaccagcgag gatatcgcct
     1021 cggtcatgac cgatatcgcc ggcggcatgc ctgaagagga agccgcgaag gcatgggtcg
     1081 atgccaaccc cgagaaagtc caggtctggc tcgggacagc ataatcccct cttttttgct
     1141 cgatctaaac gcttcacgta tcctgccggc ttgttatctg gtggcactca ctccgcctgg
     1201 ccttcggcct cctctccttc ctgaggagcg gggcagccat ggcgatgctg taggaagccg
     1261 tatcccggta gtgagccgga accctgttct ttttcaggtt ttcccatact gtggaccgtg
     1321 gggtatcgcc tcgcggggtg gggctgacnn cggccttcgg cctcctcccc cgcccacgag
     1381 gggcgggggc agtcatggcg atacctggta gaaagccgtg caaggttctt ctccagagac
     1441 ccccggaggc agggagttca ccccgcgaaa ccttatgtta atcctcccgc atttacatcg
     1501 ccaccgtggc agggtatctg ccccggtccg gatggcaggt gagagagagg ccgggcgggg
     1561 tggggtcgat gaggtctgcg gatcttcacg cgagacaggg ggggtctccc ccgccccgtc
     1621 agccccaccc ccaatggcga tagccaccac ggtccactgc atgaggagat gcccgagaaa
     1681 aaaaggtcgt cccggggaca agccgggtcc ggcaccagct tcatcagggt aaggatgaag
     1741 accagaatag ggtttcaaca gaaccctttt cgtatgaaaa tgggtcacct acatattttc
     1801 atccggtatg cctgtggccc cacgggcatc atgtggtttt tcacgacatc gaaccggatc
     1861 accccgtggg catccgcttc acggtgctcc gggcgtccag cccggacagc gtgggtccac
     1921 cggcccggag cggcgagaag ataacaatca gacaaaaaaa ggttgtgtgg gacacccgat
     1981 tacgccgggc cgatgtagtg gctgagcggc tcgagggtga tctcctcgcg cttgatggca
     2041 tcgatcgcca tcgccgcggc ccgggccgcc tggagcgtgg tgatgtaagg gatgccgtaa
     2101 tcgacggcgg cccgcatgat ctggatatga tcctgccggg acgccttgtc cgccggggtg
     2161 ttgatgatca gccggatccc gccggaccgc atggcatcga tgacgttcgg ggagccttcc
     2221 tggaccttcc tgaccagatt tgcctcgatg ccgttctggg tgaggaagtc gaccgtcccg
     2281 ctcgtgccgt agagcgcgag gccaagttcc cggagtttcc gcgcgacggg caggagttcc
     2341 tccttctgct cgtccgtcac cgagatgaag acgttcccgg tcgtcggcag ggtgttgtcc
     2401 gccgcggtgc atgccttgta gtaggcccgg ccgaagtcgt aatcgatgcc catcacctcg
     2461 ccggtgctct tcatctccgg cccgagcacg gtgtccacgc cggggagtct gttgaagggc
     2521 aggagtacct ccttcaccgc tacgtgctcg atctcgggct cggcgacgtc catatcggca
     2581 agtttcctgc cgaccatcac ctttgccgcg agtttcgcga gcgcgatgcc cgttgccttc
     2641 gcgacgaacg ggacggtccg gctcgcacgc gggttcgcct ccagcacata gaccacgtcg
     2701 tttcggaccg cgtactggat gttgatcagc ccgacgaccc cgaggccgag agcgatcttc
     2761 ttcgtgtagt cgcgcacccg cgcgatcacc gagggcgaga gggactgcga cgggatgacg
     2821 caggccgagt cgccggagtg gaccccggcc cactcgatat gctccatgat gccgccgatc
     2881 agagtgtccg tcccgtcgca gaccgcgtcg acgtcgatct caacagcgct ctggaggaac
     2941 gagtcgatca gcaccgggtg cttcttgctc acccggacgg cctctttgat ataactctca
     3001 agttcggtcg cgtcgtggac gagctccatc gcccgcccgc cgaggacgta ggacggcctg
     3061 accaggaccg ggtagccgat cgcttcggcc atcttccgcg cctcctcctc cgagtaggcg
     3121 gaactgttcg ccggactggg tatatcgagc cgggtgagga gccggctgaa ccggtcgcgg
     3181 tcttccgccg catccatggc gtcgggcgac gtgccgagga tcttcgttgc aaggccgaga
     3241 cgcttgatct ccgcgtccag cggcatggcc aggttcaccg agttctggcc gccgaactgg
     3301 accatgacac cgtagtactc gtcctttttg aggatgttta tgacgtcctc gagttgcatc
     3361 ggctcaaaga agagccggtc ggatgtatcg gcgtcggtcg agaccgtctc ggggttgttg
     3421 ttgacgatat ggacctcgat cccctcttcc tcgcggagcg ccatcaccgc gtggacggtg
     3481 cagtagtcga actcgatccc ctggccgatc cggatcggtc cggaaccgag gatcagcacc
     3541 tttctggcgc cgttccgggt cagttcgcac ccgtcctccc aggtggagta gaagtagggc
     3601 gtggtggccg ggaactcggc ggcgcaggtg tccaccatct tgtaagtcgg actcccggcc
     3661 agggccccga tctcatcgac ggtctttccc gtaagcgcct gcaactcctc gtccgagaag
     3721 ccgtaacgct tcgcgcccct gacatcctcg ggtgcaaaag cggtctcgag tttccgttcg
     3781 aggtccacga tattcttgat cttctcaagg aagaacggcg tgatggcagt gaggtctgca
     3841 acctccctga ccgtgaaccc ctcgcggaag gcatcgaaga ggcacccgaa ccgctcgtcc
     3901 gtcggagacg taaggatcat ccggatctcg cttgggctcg tgtgccgctg catatcgtta
     3961 tcgagcgacc ggagcgcctt cttgaacgcc tcctcgaccg tccgcccgat cgccatcacc
     4021 tcgccggtgc tcttcatcgc cgtggtgagc gtccggtctg cggacttgaa cttgtcaaag
     4081 ggccaccgcg gcaccttcac caccacgtag tcgatggcgg gctcgaagga tgccggggtg
     4141 acgccggtca cggggttcat gatctcgtcg agccggaggc caatggcgat cttggccgcc
     4201 acccgggcga tcgggtatcc ggtcgccttg gacgcgagcg cagacgaacg cgagacccgg
     4261 gggttcacct cgatgacccg gtagtcgccg tctttatagg cgaactggat gttgcacccg
     4321 ccctgtacgt caagcgcccg gatgatccgg atagcggcgg tccggagcgt ctggaactcg
     4381 tcgtcccgca gggtgaggat aggcgcaacg accacgctct cgccggtgtg gatacccatc
     4441 gggtcgacat tctccatgcc gcagacgatg atacaggtat cggatgcgtc ccgcatcacc
     4501 tcgaactcga tctccttcca cccggcgacg ctctcttcca ccaggacctg atggatccgg
     4561 gaacggttga ggccgatctc gatgatccgc cgcatctctt cgggcgtgtg cgccacgccg
     4621 ccgccggaac cgcccagggt atacgccggc ctgatgatca cggggagacc gacctcccgg
     4681 attgcctcgt cgatctggtt catgttctcg aggatcatgc tccgggggac aggttcgccg
     4741 atgacattca tcaggtcccg gaactgctcc cggtcttctc cccggtagat cgcctcaagc
     4801 ggcgtcccga ggatctccac gccctcgagc gcacccatct ccgcgagttc cgccgtcatg
     4861 ttcaggccgg tctggccgcc catgccgctc aggatcccgt ccggcttctc cttcttgatg
     4921 atctttgcta tgatatcggc cttcaggggc tcgatgtaga tgacgtccgc ggtatcgggg
     4981 tcggtctgaa tcgtcgccgg gttcgagttc acgaggacga ccttgacgcc ctcttcccgg
     5041 agcgcgcggc aggcctgcga ccccgagaaa tcaaactccg cagcctgtcc gatctggata
     5101 ggcccggaac caatgatgag aactttcttg atgtgtgact tcttcggcat tatggaatct
     5161 ccttatagat gcggtcaaag aagtgtatct ccgtatcccg gggcccgccg tgcgcctccg
     5221 ggtggaactg gacgcaggtg atattgaggt cggcgttctc gaacccctcg accgtgccgt
     5281 cgttcacgtt ccggtacgag acagcgcatc cctcaggcag ggtctcttca tccaccgcaa
     5341 aaccgtggtt ctgcgtggtg atgtagatgc acccgtcctg gtagcggacc ggctggtttg
     5401 ttccccggtg accgaacttc atcttgtagg tctcggcacc gagagccagg gcagccacct
     5461 ggatgcccat gcagatgccg atgaccggca attcaccggt caggtcccgg atgcaccgga
     5521 tggcgtgcgt tgccctcctc gggtcgccgg ggccgttggt gatgaagagt gcgtcgggtt
     5581 ctgccgccat cacctcgtcc ggtgtagcgg tgtgcgaaaa gacgtggatg tcggcgccgc
     5641 gggagttgag gcttgcaagg atatgccgtt ttactccgag gtcgatgacg gcgatccgtt
     5701 ttccgggtcc gctgatccgg tacggctcgg ggcaggagac gctcccgatg aggtcgacgt
     5761 cgctgatcgg ggagacggcc cgggcgcgcc ggaccgcttc ttctccatcc tcactgccga
     5821 cgatgagaga tgcccggagg gtgccggacg tccgggtctt tatcgtcaga ctgcgggtat
     5881 cgacgcccga tatcccgaga aggccgtttt cttcaaaata ttccgcaacc gagggttctg
     5941 ccgcgggcgc cgcggcgatc tcacgtgcga tgcagccgcg cgcgtgaacc cgcggactct
     6001 caaaattctg gagatcgacc ccgtaattcc cgatgagggg atatgtaaac attaaaagtt
     6061 gcccggcata actggggtca gtcaatgcct ccatgtaacc ggtcatctgc gtcgtgaaga
     6121 cgagttcacc ggaacatgcc ccctcaacgc caaaaccatt ccctacaaca aatgttccgt
     6181 cttcaagacc cagaaccgcc ttcatgaatt accataccat gtggatcaga ttccttatta
     6241 acggtaatga cggatcgaac gcccgcctct gcaccccttc tggaaacccg aaacagcggg
     6301 ctcccggcta cccgaaacat tgaaggcggt tgaacgccag gagcacatga tggtcgagag
     6361 cgcggcggag aagatccaat ccatcgagac gcctgagggg acgtttacca tcaggctcac
     6421 cctcgatgac ttaggcagca tctatccggg catgatccgg tataccgtcg aggtcctccg
     6481 cggcaccgag acggtctaca cctacgcgat caactcctac gaggcgccgc cgggctccct
     6541 cttcgatacc cgggaggtgg cggagatcgt cttctcccgg ctcgcgcacg acgttgcgtc
     6601 ccgcccccac atctacaccc atccccgtct cttcacccgc ccgctcccgg gcggcggcac
     6661 ggccgacgtc gtcatcctgc aggggagccc gcgccggttt gggaactccg cgaagattgc
     6721 gtcgtggtgc gacgacgagg ccggccgggc gggcctctct tcccgggtct tttacctgca
     6781 ggatatgaac atcagggcat gcaccggctg ctacacctgc tacgactacg gctactgccc
     6841 ggtcgacgac gatatgcccc ggatcatccg cgctctcgaa tccgcctccg tcataatcgt
     6901 atgcaccccc gtctacacca gcaccgtccc ggcgaccctc aaagcgatga tggaccgctg
     6961 ccagtggctc cacgcccggg agaaggtact ggggagcgag gtccacgccc ggggtctgct
     7021 catcgccgtc gcgggacgac ggggggccga accgtttgtc tgcgtaaccc gcgtggtcga
     7081 cgcgttcatg gagaacctgg gcatccgccc cgccgcgccg gtgctgatcg gcgacctcga
     7141 ccgcgtccgg gatgtcgggc agatcgaggg gcttgaaggc gacgtcaggg cggcattgcg
     7201 cccgctgctc gctccccgag acgaagaata atatatcccg ggtcccccac cgatgatgca
     7261 tggcagaagt gactgttgaa attgttggct ttgcggaatc cgaatgcggt ccgtttccct
     7321 gcgacgagac cagatcgtgc ggccttgaga cctgttaccc gtcgaatagc ctgatggacg
     7381 ccatcagtgc cctgcgcgac gaacttctcg ccggatacgg tgagaaggtc gaggtgaaga
     7441 cgacgctgct cgacgaggag atgcccgatc acatcaggaa gatcatcgag gagcgccaac
     7501 ctcctatccc gatcgtcctc gtcaacggtc ggctcacgtc gatcggaagg gtctcgctcg
     7561 atctgataaa agaagagatt gattatgccc ttgaggagtc ttagaagatc ttcttcaact
     7621 ctccggtcgc aaggtcgaag tacaggccgt gaagccggac ccgcccttct ttttccgcag
     7681 ccttgaccgg cggataggta cgaaggtgct caagctgcag gccgacgttc tcgatctcga
     7741 tcagccttag ccggatcttc tcttcttcgg ggttcttcgg cacctgcatt ctgccgtcga
     7801 cacggcgttt cgcctccatc gcgttgttga gccagagcgg gatgtatgca tccgtgctct
     7861 cgtggtcgag cccctttata gcgccgcagt ccgagtgccc gcagatgacg atatccccga
     7921 ccttgaggtg gttgagcgcg tactcaagca cggtcgcgaa gttccagtcg tggaccggga
     7981 caatgttgcc gatgttcctc tgtacaaaga cttccccggc cttcgcaccg gtgatgcgct
     8041 cgggatttac tctcgagtcg gagcacccta tccagaggac cgtcgggtgc tggcttgacg
     8101 ccagcgggcc gtaatgttcg gggtcttttg cgaaatcttc ttccaggaac cgcttgttac
     8161 cgtcaagaaa cttctctatc atctgtgtgt actcctgagt gattgtgggc aaccgtctcg
     8221 gacaggtacg cctgaagagg gggttacttc ccgggcttaa aaatgtatat ggctggatat
     8281 tgcggggagc agggctgcgc agaagccggg caagcgggcc gcactccggc tcgcgatggc
     8341 gcctggcacg tcggagcacc cctgtgcatc aatgtcctga tcgagggtgc agggtaccga
     8401 atgcaccacg gcaccgggag aaaccatttt aaccagtccg atttgttaat gaaaagtgcc
     8461 actctacaga gtcacagtgc cagcgaggag tgtgcgctcc cccgtgagag gggtatgaac
     8521 tctgccgaaa ccatctgttc gacaggtcaa ccgcgctccg cgacgcgatt cttcccctgc
     8581 ccggcaaaaa aagaggttac ctctggacgg cgccgaggtt cgcctgctcg acggtccgcg
     8641 cgtaccgggc gagcacgccc gtaaggcgcc gtgccggcgg tttccaggcc cgcctgcgct
     8701 cctcaagggt tgctgcatcg acagcgaggt cgaggcgctt ttcgtagagg tcgatcacga
     8761 tctcgtcgcc gtcctccaca agcgcgatcg gcccgccgac cgcggcctcg ggggcgatat
     8821 gcccgatgca cggcccccgg gtgcctcccg agaagcgtcc gtccgtcacc agggcgaccc
     8881 gggcgtagcc gaggcccatc atcgccgagg tcggcgagag catctcgggc atcccgggcc
     8941 ctccacgggg tccttcgtac cggatcacga cgacgtcgcc ctccataatc tcgcggtgga
     9001 ggatcgcctc cattgctgcc gcttcaccgt caaagacccg tgccggcccc tgatgccgcc
     9061 acatcgccgc cgggacggcg gcgcacttga cgacggcgcc gtcgggggca agcgatcccc
     9121 gcacgatctt caggccgccg gcagggctga ccggggcgtc ggccgtccgg atcacctccg
     9181 ggtcggcgac ccgggcgttc ttcgcgatct cgaggatcga gcggcccgca accgtcggag
     9241 cgtcgtcgag gtagcgctcg agcatcttca agaccgcagg gatgcctccc gcccggtgga
     9301 gggcgagcat ggagtgcggt ccgccgggct gcatgtggca gatgtgcggg gtcgcctcgg
     9361 cgacggtgtt gaaggtctcg aggtcgaggg ggacgcctgc ttccagggcg atggccatca
     9421 ggtggagcac cgtgttcgac gaccccccga gcgccatgtc cacccggatg gcgtttttca
     9481 ggctcgtctc ggtgatgata tcccgcgggc ggatgccctc ccggacgagg ccgaccgctc
     9541 gctcaccgct ctcccgggct atgcggagct ttgccgcatc gaccgcaggg atagccgcac
     9601 acccggggag ggagaggccc agcgcctcgg tcacgcacgc catggtgttt gcggtataaa
     9661 gcccctggca gctgccgcac ccgggcatcg ccgcgcactc gagctccccg agttcctctt
     9721 cgctcatcgt gccggcggcg acgcgaccca cgccctcgaa gatgtcgatc agggagagtt
     9781 cgcggcccgc cgcgtagccg gggagcatcg ggccgccggt gacgacgatc gtcgggacgt
     9841 tgcaccgcgc cgccgccatg agcatgcccg ggacgatctt gtcgcaggtg ccgacgcaga
     9901 tcaggccgtc gaaccggtgc gcctcgacca tcaactcgac ggcatcggcg atgttctccc
     9961 gtgagggaag ggaataccgc atcccctcgt ggcccatggc gatcccgtcg cagatgccga
    10021 tggtcccgaa ctcgaacgcc acgccgcccg cggccgccac gccctcctgc accttctgcg
    10081 agagggaccg gaggtgggtg tgcccgggga cgatcgtgtt atacgcgttt gctatcccga
    10141 tgaagggctg gtccatctcc cggtcggtca cgccgagcga cctgagcagc gcccggttcg
    10201 gggcacgctg gtagcctttc ttaaccgtct cactccgcat gatgatcctc gtatgagagt
    10261 aaacgcatgg ggagaaaaga gtattccggc tgtcgggtgt ggtgcagggg gaggaaggaa
    10321 tttttcccgc acgacggggg cgagcgatat cccatgtttg agaacgcttt cgttacacac
    10381 ccttcaaatg ctcgtatcct acggtcaagc ggacccggac acggatttct ggcggatatc
    10441 gccacgcact gcccccgccc tagggggcgg gggaggaggc cgaaggccgg gtggggtggg
    10501 ggttatagag ttcccagatt ggtggagaca ggggaggggg gatgctcctc cctccccgtc
    10561 agcccctccc ccaagggcga tacccccacg gtccagtgta cgaggttcta tcagagttta
    10621 ccagttctgt gtttcggggc tacgcttaca ctctggtgct catgttgctg caaaaccgca
    10681 tgaaaccggg aacgatcagc gcaaaaaaag acggtaatta tatcggcaag gggagccgcc
    10741 ccctcacccc tcaaccccgt catacaggtt caccaccccg ccgatgacga tcaccgccgg
    10801 gggccggacg cctgcggcac gggctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggaggttctt tatccccatc aggatgacga
    10921 gggtcccctt caggcgcgag aggacctccc agtcgagggc ggactcgggc ttcgtggggt
    10981 cttcgtggcc ggtgatgaag gtcacctgcg a
//
LOCUS       DAJB01000010            3853 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_96900, whole genome shotgun sequence.
ACCESSION   DAJB01000010 DAJB01000000
VERSION     DAJB01000010.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_96900"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    641..677
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2678..2735
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          654 a         1171 c         1164 g          769 t
ORIGIN      
        1 gcccccctcc ccgtcagccc cccccccaat ggcgatagcc cccacggtcc acggcatgag
       61 ggcatgcccg agaaaaaggt cgtcccgggg acaagccggg tccggcacca gcctcagcag
      121 ggtaaggatg aagatcagaa cagggtttca accaagccca aaaattctct tacttttctc
      181 tgcgcccggc cggaaagaga ggcggggcgc gagccccggt tcaccagact tccggaaacg
      241 ccatgttggt gtagatgtcc tcgaggcgtg ccttgtggcc ccgctccatc gcggcgaggc
      301 tctcgaataa cgccatctgc tcggggtcca cgctcaggct cgcgagctgg gtgtacatct
      361 gcatcgcgtc gagttccttc cggatcgcga taacgaggcc gtcgagcggt tttaagtcgg
      421 cggagagcgg cggtgtctcc agggtctcgg ccaccttgta gtttcgctgc gtgttaaagc
      481 cgagtttccc cggctccttc atcttaaggc cctccagggt cttcctgtgg ttgctctcct
      541 ccccggcaag ctcgttgaag aggttcttta aattctcatc cgtgaccttc tccctgactg
      601 tccggtagaa ggtgtaagcc tcgacctccc ggtcgatggc nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnggg cgggtgccgg gacgtcatgc tgtggaccgc cccggggcgg
      721 ttatgtgatt tccggaagaa cctgcagcca caagacctgg aatgataggt ctgcggagag
      781 aacgcgatcg aaatgccggg gcctccttag gtggcctcgc gaaaaaagag tggttagatg
      841 aaccggggct tgttggagag gagcctcggg gtatagaggg gccggaaggg ctttccggcg
      901 gtctcgagct tgaccgcctg gatgagctcg gtctcggtca tcgtctcctt gtagggcttc
      961 ttcttctccg agagtttccg gatggtgacc atgagccggc cggtctcggc ctcctggtcg
     1021 ccgatcaccg cgacgtaggg cacccagtcc atcccggcct cgcgaatctt cttgttcacg
     1081 ctctcgtccc ggtcgtcgac gtccgcccgg atgcatgcgg cattcaggcg ggcgccgatc
     1141 tcctcggcaa agcagacatg ccgctccgcc accggcacca gcctgacctg ggttggggcg
     1201 agccaggtcg ggaacgaggg gacttcctgt gcagcagtgc cctcgagcat cgcgcagatc
     1261 acccgctcga tcgagcccgt cggcgagcag tggaggatcg gggggtggat ctccgttcct
     1321 tcgggggtgt agtacttgat gtcgaaccgg tccgcgctct cgacgtccat ctggaccgtc
     1381 gggttctcga tgggtctccc ctgtgcgtcg atcgccgcga ggtcgacctt tgctatccag
     1441 tagtggaccc ggtccgagag gacttcgatc agcatcggca cgcccgactt cgcgacgatc
     1501 ccttttatcc agagttcata ctggtcgaag aagtcccggg tgcaccggaa gactcccacg
     1561 agcggggtct cgaggtcttc gccggtcttc cagccgatcg cgagctgctc ctcaaacgcc
     1621 acaagcgccc cgtccgcgtc ccggcagagg gtgtgcatgt caggcatcgt gaaggcgcgg
     1681 agacgcttca gtccgatcac ttcgcccttc tgctcgtgcc ggaacgagta ggtcgagagc
     1741 tcgtagagtt tcatcgggag ggtgtttggc gagatgtgca tgtcgtgcat gatcgagaac
     1801 atcccgaaac acgcagcgaa ccggagcatc atgtcgcggt tgccgctctt gaaccggtac
     1861 tgccgttccc cgaacttggc ggcgtgctcc gcgatcgcct tatcgccgag gtcgtacatg
     1921 accggggtct ccaccggcat cccaccataa tcgagcacca gagacaggac gtagtcggaa
     1981 agaaggtccc ggatcagctt gccccggggc atccagcggt gatgtccgac gtccgagcgc
     2041 ggctcgtact ccacgagttc cttggcccgc atcaggtcta cgtgaaccgg ctcggtccct
     2101 tccggacccg gatacccgag ttccttccgg accagggcgg cgaagggcgt atcctccttc
     2161 gcgtattcgg ccgcgtcttt gcgctcccct tcgggggtga aaacgaagaa ctcgtgctgg
     2221 atctccttct tttcggcggc cgccgctcca tcgcccggga cgatcgtccg ggagagctcc
     2281 gagagcgggt gacccttgca ggagagcgag aaagccttgt accagccgaa cggcgcccgt
     2341 ttcacaacaa agccgccctt cacggcgagg gcctcctcga taccccggag agcgctcact
     2401 gctgtttttg gcgatgcaag gtcggaggag aggtgggcat acgggtagat catgatattg
     2461 gtagtgccga gctgcccggc ggtggtgacg atctcgtctg tcgcctgcct gacaacatcc
     2521 ccgatattct cctcgtcgac cgactcaatc gcgcagaaga cggcgagcgc ctcatcgagg
     2581 gcgtctttct ggatgacgtc ctcttcggcg accttcgtct tcttccgggt ctcgtattcg
     2641 atatgatcag agtggattag aagaagtcgc attggtannn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagag ggtcgcctgc cgctggtcca
     2761 tgtacttcgc gtcccgcttc atgttgtacg ggcgcgcggc gcggtcagtg cggtagaata
     2821 ccagctgccc gatcggcatc cccgcgtaga cccggaccgg ccgggagttc acgttgcaca
     2881 tctcgagcgt gatcgtcccc cggaaccctg cgtcgatcca gcctcccgtc tggtggagtt
     2941 cgacccccag gcgggcgatg ctgctcttcc cctcgatgct tgccacgacg tcgtcgggga
     3001 gttcgatcga ttccagggtc tcggcgagga ggaaccgtcc tggagtaagg acgatcgagt
     3061 cggctaccat ctcctcggtc tccgagcaga cgctctcctt ctcgtaaggg tcgatcacgg
     3121 tatctcccgg aacataccag acaaagtggg aaccgagcct gatatccagg gagttgggct
     3181 ggatcagacc gggctcaaag ggatctacct tgatataccc ccgcctgatg cggtcttcta
     3241 tctgccagtc gacaagaatc attctcttag atttctcctg caagccagtt tattctttgt
     3301 tttcaaggtg ccactccgtc cggcgcatca gcccatgaag cgtgcttgcc tcgcggatgg
     3361 tcagcttcgc ccggcccagc acccggtgga tgaggagcat cgtgttctcc cgcttaaagt
     3421 ccggatggtc gacccggtcc aggaaatcat cgatatgccg gtagagcgac tccatctcga
     3481 cggggctcgc gaggggatag gtcccatggg gcaggttcgc aagttcgtag cagaggatgc
     3541 ctaccgcgtg ggagaggttc aggataggat acacctccga ggtcgggatg gtgcagatga
     3601 ggtttgcgcg cttcagttcc tcgtttgaga ggccccagtt ctcccgcccg aagaggaccg
     3661 cgaccgtgcc gtcgatatcg gcgacagcct cccggacctc cgccggcgcg tagtagggca
     3721 tccgcatcgg ggtgcagacc gacttgctca cctcgccgct cgtcgcgacc acgaaatcga
     3781 actcccggta gacctcgtcg atcgtcatcc ggcgggcaca gtccagaacg tcgcgggcat
     3841 gggaggcgcg cat
//
LOCUS       DAJB01000011            7782 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_34443, whole genome shotgun sequence.
ACCESSION   DAJB01000011 DAJB01000000
VERSION     DAJB01000011.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_34443"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1519 a         2270 c         2299 g         1694 t
ORIGIN      
        1 ccccatggcg atagccacca cggtccacgg catgagggca tgcgtgagaa aaaccacatg
       61 atgcccgtgg ggccacaggc ataccggatg aaaatatgta ggtgacccat tttcatacga
      121 aaggccgaac tcaggaccag gccaggtccg gcaccaatct cgtcggggaa gaatgacagc
      181 cggaataggg cttcaccaga gcccggtaag ggaaatcttc tccccatgtt attttttgtc
      241 atgaacgcgg ggtggtgcgc aacgtaattc ggccggattc tctgagatca attatcaggt
      301 atatgctgga gcactacccg catgttgcgg tgcaggttgg cggagacatc gaaaaagccg
      361 acctgaggga agcgatctgg ccgatagccc ggtatatcgc cactatggtc ccgaatcgac
      421 cataaaggtt ttatgactcc catgataagt actgtgcaat gaagtgggtg accatcggcc
      481 tggtgctgat gctggtatct gctctcgttg ttcccgcatt ggcggcagat gaggggcggt
      541 acagttatat cacagttaaa gacgtcaccg tccggctgga gaaagcggat gccgtcgtca
      601 ccatgaacta tacgatagac ggcggggtcg ggttcctcgt tctcctcctt ggtaaatccg
      661 atctgaagca gaaatcactc gatatactga acttcaacga caccagtgtt cagcgtctcg
      721 atatcgagcg catcgaggtg cgtgtgaata acgcctcgga cgactacggg caggggtcct
      781 actggttccc cgcgcacagg ttcggcgtgg tcgtgccctc gctcaccgtc gtcaccccgc
      841 aggacgtcaa acggtacgag aatgtcagtg aattccctga cggactcggc tattttgcgt
      901 gaaccgtgaa agcggtcgta tctgcgatac ggattacggt gtcggtcttc tgacgctacc
      961 agcaggtgtt tcgagtacgg catcaggcgt tgcggccgcc cgcctgtgcc tgcggcagca
     1021 acccgatctc cgccagggcg cggcgggtgt ggcgggcgaa ggcagcatct tttggcgacg
     1081 ccgcgacctc acggcagagg cggtccaggt cggccgccgt gtcgacgtcg taccagggtt
     1141 cgagcaggga gacggatagg tcaaggcgtc cggcggcttc gaccgtccgc tgcgtcacac
     1201 gggccgagct ccaggggatg ccggcaaaga ggcggggggt atgcctccgc atcccgatca
     1261 ggtagtagcc gccgtcgtcg cacggcccga cgacgacatc ggcctcgtcg agccttaaga
     1321 atgcctcccc gatgtaccgg ccgggaaggg tcggactgtc gctgtcgcag agcacgaccg
     1381 ccgttgcccc ccgggaaaag agcgcttctg aaatgttggc aaggcgttca cccaggtctt
     1441 cgccggcctg ggggaggatg gagaaacccg gggggacgat gctggcgaag aaatcgtccg
     1501 cagcaggagg cgtgtaagcc acgaacggac ggacaccgtt gatgcgggcg agccgatcga
     1561 ccgcgtcccg caggaaaccg gtgtacagcc gcgctgccgc gagatgcgtg agcggcggga
     1621 cgagccgcgt ttttacctgc ccggggattg gcatccgggt cataatagca gccgcgatcc
     1681 cctgctctac ctgacgagcg ggtttgggaa cctgctggca gcgaggacgc agaccttctt
     1741 cggggacttc ggcatctttg tagaaaaaga ggctttcttt gagatcgatg gatacgagtt
     1801 gcttccctat ctggaggacg tggagttctt ccgggcggcg cgccggcacg agaggctcgt
     1861 ccaggtcgac tgtcccatca ccgtatcgcc gcggcgctat ctccaggtgg ggaagtaccg
     1921 cctgagcgct gtctatattc tggtgatgct cctcaatacg gtcggcatca ggccgaagct
     1981 cttcattcgc tacgtcgtcg agaaatagaa tgagatgtcc ggttccggcg tcttacacct
     2041 ctctcttcag cgggacctgt tcacgatcgt ccacccactt tcccaggaac gactggaggt
     2101 agacgccgaa gaaggcgcag aagaggcccc cgattgtcgc gaagatgatg tactttacac
     2161 cgacgtcggt cgttataggg aacccggcga tgtcgctgat cgagaggatg tagatgcttg
     2221 caccgtaggc gatggcccct atcgccagca cgaagaacgg cagggcgacc acccgctgga
     2281 ttgcctcccg ctcgtttagg aagacatcga tgatctttcc taccgatgca acgaggccgg
     2341 cggcggtgaa ccagacaatg gcaccgtaga tgaacgcaag gacctggaag aagatcccgg
     2401 cagaggtata atagtcgagg aggcttatga gcccgaggac aacgccgacg atacccagca
     2461 gtattgcggt gatgtaggag acaaaggtga acctcccgcc atgcagcgac gtccggagcg
     2521 agttgatcag atacccgaag acctcgtcga tccccatgcc cttgaagagc agataggtcc
     2581 cgatgatacc gacgacgacg atggttgccc cctcggggta tcccagcagg tatccgactg
     2641 cgtaaaggag catggcaaga ccgagcggta cgagcacgat cttcgagact tttggatcgt
     2701 cgagaagacg cctgaggatg tagtacgttc cctcgaggtt cggcatctgg ttgaccacga
     2761 ccctccgtac gctgctgacc ggcacccggg actggatgat cggcaggaca tactcgtcct
     2821 cagcaccgtc cgtcaccaga atgcaggctg tagaatttgt cgcggagagg attgtatcga
     2881 gactggacgc tacccgccga tctccctcga gcaggttcat atggttgccg cagatgacag
     2941 cgatggcaac ctcttccccc cgcctggtga gttcatcata gagtttgatc gtctcgaaga
     3001 tcgcgttgat gtcggagtcc tcaggatcaa tcagagcgag agatgtcgcc gcatggaggc
     3061 atgcctccct gcccacgatg gggccatcga tccgggcttt gaacccgaga tcatcgtcgc
     3121 gatcgacaca gaggacgagc gtccgctctt ttaccattca atagacatct cctcgaatct
     3181 attaaataat ttgcagcggc gggtaaaaaa gtattaaatg agttttgcac gctgaagcag
     3241 gagcaggtcg tcggtcgtga tcttttcacc ctcacggaac atctggaaga cacgttccgc
     3301 ttctttcctg acggctttct gctctttggt gactttggtc tttctggtct tcttccgcag
     3361 acccgagatc accttgtcat aatcccggag ttccttctgg caggtgatga actgcttgtg
     3421 ctcctcatcc gccgactcct gggcttcgac gaactgctgg tgggccagat cggcctcttc
     3481 gcgggacttg tcggccttcc ggtaggactc caccatcagg tcgtggtgct gctgtgcggc
     3541 ctcggccttc tcggtcactt ccttgtggat gtccgaggcc agcctgcgaa actcacgggc
     3601 atcggagagt ttcgtccgca tctccttgtt ctgctcgagt tcagcttcct ggtccttggc
     3661 cgtcgccttg agctgcttga tcttgtcgat cagttcgcgc tctttatcgg tgctgtagac
     3721 ctcggtctgc tgcttgaact ccaggtgctc gatctgcttc tgcagttcct tgatgccccg
     3781 gttctggagg ttgccgtgct ccttcttgaa ggactcaatc tcttcgaaaa gcgtgtttgc
     3841 cttgtcgttg tactcgttcc tctggtcttt taacgcctgg acttcctcgt tgatcttgtc
     3901 ccgctgctcc ttgtgctgct gggcttcctc gacgaattcg cgggtctgcg tatttaacgt
     3961 gttgcgttcg cgggcgtttt tgctggcaag cgcgttcaac tcattgcgcc ggtctttgtg
     4021 ctgttcagac tccgcaagga ctttttttct cttctcaatc agatcgttca gcattctcta
     4081 ccaatcctcg acattcggta cctgtctcat atccccacca gcgggaccgc tcccattgcg
     4141 ctgcagggat gcccgaggct tatgtagcgg ctacaccaca agaaaagacg tttccggacc
     4201 cggcaatcga gaccgtcgtt catctgtctg ctcatgtgtt caacctcggt acttggcaat
     4261 cgcagacccg caggttctgc gccctgcaca atacaccccg tgctggaggg attatacaag
     4321 aacgtctata attcaaacgt catctgtact attaagtatt tcgcaggggg tgcgaagggc
     4381 acgccgccgc caagcgattt cggccaaaaa ggtatgggaa gagggtatgg agtattagag
     4441 gaagtcgatg aagtgcccgc tcggggcatc cccggcaatg cgcctgccgg caggtgcgcc
     4501 ggaccgctgt gttgccggtt cgtaggtgcg gtgacttccg aggatctgtg cactttttac
     4561 gccggtcatg actgccatga cgcgaactct tccttcataa tcgctgttga cccgtgctcc
     4621 ccagatgacg tccgcgtggg ggtcgagttc gtaggtcaga gagcttgcga tctcctcggc
     4681 gtcctggagg gtcaggtcgt tgccgcctgt gatgtggatg aggctccccg ttgctccccg
     4741 gtagtcgatg tccagcaggg ggtggttcaa gcactcgtga acgacgctct cggccttgtt
     4801 ctgctgcttg ctctctccga caagcattac ggcgacgcct cccttgctca tgatggcacg
     4861 cacgtctgcg tagtcgatgt tgatgagcga aggctccgtg atcgtctcgc tgataccctt
     4921 gacggtctcc gcgatgagct ggtccatcac cgagaacgcc tggccgagcg ggaggttcgg
     4981 cacgtacttg atgaggcggt tgttgtcgag cacaatcacc gagtcggcag aggccgagag
     5041 taattcgagc ccttcttctg cacgaagaag ccgggccttc tcgacctgga agggataact
     5101 gaccataccg acgacgatgg ctccctgctc cttggctatc tgcgcgacaa cgggggctgt
     5161 tcccgtaccg gtacctccac ccataccggc agtgatgaag acaaggtctg cgtcacagag
     5221 cagggtctcc agggttggtc gggccatctc ggctgcacgc ctgccgacgt ccgggaaccc
     5281 accggctcca aggcctttgg tgagcgactt gccgatgagc actcgcttgt cggcctggac
     5341 catatccagg tgctgcttgt ccgtgttgat cgcgatcgtc tccgcaccgg tgacctgcat
     5401 gtggtacagc cggttgacgg tgttgttacc tgcaccgccg catcctacga tgacaatacg
     5461 cggctggccg aggatgtcat cttcctccgc cgagtccgtc ttcaggaatt tctcccgttc
     5521 tgcatgtttt aacgcctcgt tgatgatcgt ctgcatcaat ataacctcct aaactttgaa
     5581 tgaaatctgg tcggcctcgc ggagaacccg gtttccatgc ctctcgagaa gccctctgac
     5641 cgaataccgc acagcctccg atactgtggg gaattcacca gcttccacca gcctctcgag
     5701 catctcaacc tgctgccggg gcaatctgat agttatccga tccatcattt caatcactca
     5761 catctgacat ctggcagcct tttgtctgct gtctgtcaga cattcgtaat cttttcgtct
     5821 gactgcatca gataagccat aataatattc gtattacata tataccttcc gatgtgaatt
     5881 cctgcccgac ataacggttt tatggatagc gaccgacaac gtatcctcaa agggttgcca
     5941 ctgacatgag agataatctc cctaaaaggg cggaacatgg cggccgggtg cgctggcatc
     6001 gcacccggga gagaggcgag ctgctcgatt tcagcgcgaa tgtgaaccca tatcccccgg
     6061 agataccctg gaagccggat ctctcgatac tgaaagatta cccggatgac cggtatgaga
     6121 cgctcaaaga ggtgatcggc aggacattcg ggcgcgatgc ggcagaggtc accgttggca
     6181 acggatccgt cgagttgatc cgcgcgttct gttccgccat gctcgggaac ggagataccg
     6241 cctgtatcaa ccccccgacg tttgccgagt atgcgatggc ggtcaggctt gccggtgccc
     6301 ggtgcactgt cgatggcagc ggggccactg ttctattcct ctgcaacccg aacaacccca
     6361 ccggaaaact gtatacccgc tccgaagtcc tcaacctgct tgccgcggtc gccgggcagg
     6421 ggacgtgcct cttcctcgac gaagcgttca tcgaactctc cgacccgcgc cagagcgtcg
     6481 ccgacgtctc ccatgagaac ctctttgtgc tgcgctccct gacaaagagc ttcagcgtgc
     6541 cgggcatcag atttggatac gggttcggca cgcccgaact catcgaaaag gtagagacga
     6601 tgcgccttcc ctggactgtg aatgcgtttg ccgagtcctt tgcgatcgag gccttccggc
     6661 actatgacag gctcgaagag tcccgggagc ggattgcccg ggaacggacc tggctctccg
     6721 gctgcctgga tacccttggc ctcgcatacg agcctccgtc tgcgaactat atcttattgg
     6781 aggtcccgat ggaggcggag gtgctggtcg cggggctcct ttcccgcggc atcctggtcc
     6841 gggactgccg gtcgttcggg ctcccccgcc acatccgcgt ggccgtacgg acccacgagg
     6901 agaaccggca actcatcgag gcgctcgaag catgcttgcc ctgatcatgg ccggggggca
     6961 ggggtcccgg ctccggatgg gcgagaagcc gctcgttacc atctgcgaaa gaccgatgct
     7021 ctcctacgtc atcgatgcct tttcgtccgc agggcacgag gtggtcgtgg tcgcctcgca
     7081 caaaacacca ttcaccaaaa actggtgccg ggcaaggggt ttcctcctct acgaagccga
     7141 agggcttggc tacgtcgagg atatcgatgc cgcggcggcg gatctcgggg aagaaggaac
     7201 gcctttcttc acctgcgtat ccgacctccc ctgtcttgcg cccgatatca tcgccgccat
     7261 cgaggacgcc tggcgccggg cgggaacgcc ggcatgctcg acgtgggtgc cccgcgctct
     7321 ctgcgaggag catggctgcc gcacgcagta cacggagacg gtcgacggca cgccggcctg
     7381 ccccgccggg atcaacattc tgacggcggg cgatctcgat ggcccccagg aagaactgca
     7441 gctcctcctg cgcgaccgga ggctcgcctt caacatcaac acgcgcgaag aactggcgct
     7501 ggtgcaggag tacctctgcc ggaagttgga cgagtaggtc tgcggttgtg ccggggccat
     7561 gctgctgcac gattgaagac gttcagcccc gccgaaagga agaaatttgg gctctgctga
     7621 aagcgtgttt gggttctgga ggtcgtaaca gagggacgtg tggatgtccg gatttcaagc
     7681 acctaaagtg cgaggtgcaa ctgtcggaac gacaggagtt agtcaactac ccccggttaa
     7741 aaccgggggc ttgcagcggg accccttgcg gggcacaggg ct
//
LOCUS       DAJB01000012           11332 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_106445, whole genome shotgun sequence.
ACCESSION   DAJB01000012 DAJB01000000
VERSION     DAJB01000012.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11332
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_106445"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2338 a         3387 c         3454 g         2149 t
ORIGIN      
        1 ccgagcattt ttctgcaatc ggatacgatc actgatctgt gagttccatg gagatccagg
       61 taatcggtgt tcagggcctc cccctgatcc agaaaggcga cgatctgcct gccctgatct
      121 gtgatcgggt cgcgttcgaa gacggagata tcctcgccat cgcctcgtcc gtatactcca
      181 aggcggcggg cttcacccgt gagctcgcca gcatcacccc cggcgcggac gccgtgcgga
      241 ttgcggcgaa gacgaaggaa gacccccgct tcgtgcaggc ggtgctggac tcgtccaccg
      301 atatcctgct cgagcacccg ttcatcctct cggaactgcc ctccggccac atcggcgtgc
      361 gggcaggcgt cgaccatagc aacgtcgagg acggccggct gatcatcctc ccgcccgacc
      421 ccatgggggc cgctgtcggg gtccgggacg cggtccgccg catcaccgga aaggacgtcc
      481 gggtgctcat caccgacacc tgcggcaggt cgtttcggcg cggccagacc gggattgcca
      541 tcggatgggc gggaatgacg gcaatagacg actaccgggg cgacaccgac ctcttcgggc
      601 atgtcctcga gatcaccgaa gaagcggcgg tcgacgaggt tgcagccttc gcgaacttcg
      661 tcatgggcga gagtcaccag ggtgtccctg ccgcggtctt ccggaactgc aagacctgga
      721 acgggcacga ttccgtctac ttcacgcccc aggaagatat catcagggca gccctgaaga
      781 agaacaaaaa ggattagaag agagcattga ggataaagac tgccactccg gtcacgatgg
      841 tcacgatgat cacggttatc gggctgatat ggatggcccg gcgatcttcg ctgtcgtagt
      901 agttgaccag gccggcagag gataccagtc ttccgccaga ttttttcgcc atactaaaat
      961 attctccctt gcaatatata aaacagaggt taagagagat gcaggaagtc acaccgtacg
     1021 tcgtcaccgg ccgcacactc ctcggcgaag acctcagaga agaagatgtc gccatcaccg
     1081 tctccgcggg tatcatcacc tcgatagagc cctatcccgg gatgccggac cggtggatcg
     1141 tgcccgctct cttcaacgcc catacgcacc tcggcgacac cgtcgcgatg gatctccccg
     1201 cccgcggcag cctcgcggac ctcgtcaaac cccctggcgg tctgaaacac aggatccttg
     1261 cggcaacccc gcgggccgaa ctcgtcctgg ggatgcgctc gagtatcatg acgatgatcg
     1321 cgaccgggac cgccgggttt gccgacttca gggaaggggg ggccgacggt gtggcggcgc
     1381 tccgcgaggc tgcggccggg ctcgactgcc ggcccgtcat cctcggccgg gaaggcggag
     1441 aacaggtgag cgacggggcg ggcatcagca gcgtccacga tgtggcaaac gccgaagaga
     1501 tcgtcaggga tgcacgagcc gccggcaggc ttgtcgcctt ccatgccggg gagaagaacc
     1561 cggacgatat cgaagaagcg ctcgcatttg agcccgatct gctcgtccac tgcacccacg
     1621 caacagacgc acagctgcgg cagatcgtcg atatgggtat tcccatcgtt gtctgcccga
     1681 ggtcgaactg gctgctcggg gttgcggcgt cggcggccca cccacctatc gcgcgcatcc
     1741 tcgaacttgg cggcaggttt tatttgggga cggataacgt catgtttgtt cagcccgacc
     1801 tgctgcagga gatggctttt accgcaaccg tctaccgcgc gccacccgaa gagatcctgc
     1861 gtgccgcaat ctcgggggcg cggctcgccg ggagatcagg attcctcgat gaggggcagg
     1921 aagcgcattt cctcgtcgta aacccggcgg aaaggaatct ctccttcagc aaagacatcc
     1981 tggcaaccat cgtaaaacgg ctggattctt catctatctg ccaaacagtt ttaaccctgc
     2041 aatagaaacc aaataagaag tatctgttct gcgtggtggt gtgtatgttc cgtaagatac
     2101 tcgttgcgat agatggctca gaaccggcca accgggcttt cgaagaggct atcggtgagg
     2161 ccggcgtctg ggatgccgaa gttcatgtcg tttatgtggt tgaatccggg ctcttttcat
     2221 cccttcccat ggacaacaca ctcgaaataa tctacagtgt tttgcagaag gaaggcgagg
     2281 aaattcttga agcggctcgt aagaaagctg atgcggcagg cgtttcgctt acgacgcatc
     2341 tccgtcaggg gcacgcgggc tcccagatcg tctccctcgc cgaagaactg ggagccgatc
     2401 tcatcctcct cgggtcctac ggcaagagcg gcgtcgaccg tctcctgctc gggagtgtga
     2461 ccgactacgt ggtgcaaaac agccccatca cgacaacggt ggtgagatca tagtctcccc
     2521 tcagcagatg ctcgtccgga actttatggt gacggacgtc gtctgtgttg agatccccgg
     2581 aaatagggac gatgtcctgc gtatcctgaa acgcacgggc ataagcggcg tcccggtttt
     2641 gaagaacggc gaactcgtcg gcatcatcac ccgaaaggac ctcctccgca aagcggacga
     2701 gacccagctc gggctcctga tgacgcctga cccggtcgtc atcaggcccg acgccccgat
     2761 cacagaagcg gcccagctga tggtgcggca taacatccga aggctccccg tcatggaagg
     2821 cgggtcgatg atcgggctca tcagcgtggc cgacctcatc ggcgccatcg cccagatgcg
     2881 ccttaccatg cccatcaagg acaactacgt cagcaagacc tacgctctct gggaggatac
     2941 gcctctctcg ctcgtcggaa gggtccttga gatctcaggc tacgatgcca tccccatcct
     3001 gatggaagac ggcacgctca ccggcatcat atcggagcgg gacctcatcc ggcattcccg
     3061 catcgaggac ggcgtcgagg tcagcgactt ctcgaacggt acggacgacg atgagtggac
     3121 ctgggagagc atccgggaca tgcacaccat cagttacggg atctccagga tccagctcct
     3181 tccgatcccc gtgaagaacg cgatggtgaa aaacgtcctc gctgttccct taaacgcaga
     3241 ggtcggcgaa tgcgcactca agatgaggcg atcccgggtc gaccagctcc cggtggtcaa
     3301 cgggaacaag cggctcgttg ccatgctctt tgatcgggag ctgatcaagg tcctgctgcc
     3361 tgacccacag ggcctgcgaa agagttaagg agcaacattc atataacata cagatgctta
     3421 tcgtgtcgtg ccataaccgg gtacgacgat aaatttatgt tttatggtat cgctatacgc
     3481 ttaaccgctt ttgagcgaga taaagcgttt taatggtttt aggggtgctt caatgcttga
     3541 tacttcccaa gagattaaga aaggtacaac cacagtaggg ctgatctttg atggtggcgt
     3601 agtcctggta acagagatgc gagcgacgat ggggaacatg atcgcgagca agcgtgccaa
     3661 gaagatctac cagatcacgc cgagaatcgg tctgaccatc gccggcggtg tcggtgatgc
     3721 gcagcaactc gtccggatcc tccaggtcga gtgcaacctc tttgagatgc gccgcggcaa
     3781 gaccatgtcg gtcggtgcgg catcgaccct gctctcgaac tacttaaacc agaaccgcta
     3841 ctatccctac tacgtccagc ttctgatggg cggattcgac aacgaggggc cgagcgttta
     3901 ctccgtcgac gccatgggtg gagcgaccaa ggaagaggat atcgtcgcaa ccggctcggg
     3961 ttctcccttc gcctacggtg tgctcgaaga ccagtaccgc cccgacatga aggaggacga
     4021 cgcgaaggac ctcgccgtcc gcgctgtccg ttcggcgatg cgccgtgact ccgcatccgg
     4081 ggaggacatc atggtggtcg tgataacgaa ggacaaatac gaagaacaca tagagagcgg
     4141 gctgcagagg ccatctgtgg cccgcccgac accctgacat tttttaggac gattttaatg
     4201 gtgattgaag agaaactcca ggagcttaag gagcagatca acaagattgt tcccggtggg
     4261 attacaatct caaatgtcga atttgagggc cctgagctcg tcatttatac cgatgacccg
     4321 aaacagtttg ccgatcaggc cgacctgata aaaatactgg cacgtgacct cagaaagcgc
     4381 atcgtcgtgc gcccgaatat ccttgaagac ccggaacggg cggcccagga gatccgggcc
     4441 gttgtgccgg agggtgcggg gatcaccgat ctcttctttg acccggagac gggcgaggtc
     4501 ctgatcgaag cggagaagcc cggcgtggtc atcggcaaga acggcgtgac gctccgcgac
     4561 atcacgaagc agatcggctg gacgcccaag gtggtccgga caccgcccat cgagagtacg
     4621 acggtgaagc agatccgtac ctacctccgg tcggtgaagg acgagcgcaa gacattcctg
     4681 cggacgatcg gccggcgtat ccaccgggag gtcacgagca aagaccagtg gctgagggtc
     4741 accacgctcg gatgctgccg ggaggtcggc cgtgctgcgt tcctgatctc gacgccggag
     4801 agcaagatcc tggtggactg cggcgagaag ccgggcagcg ccgcgaacgg gacgccgtac
     4861 ctctacgtgc ccgagatctc tccgctcagc tcgctcgacg cggtggtgct cacccacgcg
     4921 cacctcgacc actgtgctct cgttcccctt ctcttcaagt acggttacga gggcccggtc
     4981 tactcgaccc ccccgacgag ggacctctcg acgatgctcc agctcgatta cttagacgtc
     5041 gttcggaagg agacggataa gatcccctac acctcgaacg aggtgcggac ctacatgaag
     5101 cactccatca ccctcaacta cggcagcgtg accgatatcg cccccgacgt caagctcacc
     5161 ttccataacg cggggcatat cctcgggtcg gccatcgcgc atttccatat cggggagggg
     5221 ctctacaaca tcgcgttcac cggggacttc aactaccaga agtcacggct gttctccccg
     5281 gctgtctcgt ccttcccgcg gcttgaagcg ctcttcatgg agagcaccta cggcggggcg
     5341 aacgacaacc agccccagag aaaagatgcg gaggataaac tctacgaaac ggtctcgacg
     5401 acgatccagc gcggcgggaa ggtgatcatc cctgcgtttg ccgtcgggcg gtcgcaggag
     5461 gtcatgctcg ccctcgagga gggtatccgg aagcagagga taccgccggt gaagatctac
     5521 ctcgacggca tgatcaagga agcgacggcg atccacacga cctatccgga gtacctcaac
     5581 agcgatctca gaacccagat cttccgcgag gggttgaatc ccttccttgc cgaggccttt
     5641 gtccaggtgg actcaccggt cctccgtgag aaggtgatct cgggcgaacc ctgtgtcatc
     5701 atcaccacga gcggtatgtt aaacggcggt ccggtgatgg agtacctcaa ggccctcggc
     5761 cccgacgagc gcaacgcgct gattttcgtc ggctaccagg cagaggggac gctcggacgg
     5821 cgtatccaga agggatggcg tgagatcccg ctcggatggc gcgagacgat cgtgatcaac
     5881 ctcgagatcg cgacgagcga cgggttctcc ggccactcgg accggaagca gctgatgaac
     5941 tatgtggccc acctccagcc gcgcccggag aagatcttca ccatccacgg cgacgagaac
     6001 aagaccctcg accttgcaag ttcgatctac aagcgctacc gtatcgagac gcattccccc
     6061 atgaaccttg agacctatcg catgatctaa ggatgctccg aaacctgccc attctcttcg
     6121 ggatattcgt ggtgatggcg ttatcaaacg ccgtcgtccc ggtgctcccc aattttgccg
     6181 aaggggcggc actgcagggt gcggtctacg ccgcatactt tctgggcacg tttgttaccg
     6241 tgctgccggc agggatagca agcgaccgga taggccgcgt gccgctcatc cgtgccggcc
     6301 tactcctgac cctcgtaagc ggtgtcctca tcctgctctt cccctcaggc ctgccgctcc
     6361 tggtattccg gggagtcgag gggatcggag ccgggctctt cttctcggcg gcgcttgcgt
     6421 gggcaaactc gcacccgtcg tccgggcaga tcagcggcta tgtcatggcg gccttaaacc
     6481 tcggcctggt cgtggggctc ctcgcgacag gctggctgga tgcggccctc gacagccatc
     6541 ttgcgggaat cgctctcttt acggtgctcg ccgtccccgc gctcgggatg agtttcgcaa
     6601 tccgggagag cgagcgcggg gagttcgcga gagccgacct tcctgcaatc gtcagaaact
     6661 acttctggct ctttgtcgca gcggtcgtcc tcgtggggat gaccggggcg gtggcggcca
     6721 tctatcccgg gtataccggg aacgatccgt cgctccttgc tcttcagctc gcgacgatca
     6781 atctcgcgac cgtcgttgcc ctgatcgtcg ccccccgttt tgccctccgt ccggtcccga
     6841 ccatccgggt ctcctcgctc ctgatggccg gggcggtcgt ggcgggttat tttacgccct
     6901 atgcctttcc cgtcatcggc gcccttgccg gggtgatcat cgtcgcggct cttgcgttcc
     6961 ttgcagagac gaggatccag cagggcgtga tgaccgggct cttcaacacc gccacttacg
     7021 ccgggttcac gcttcttccc gcccttgccg ggctggtcgc gggagaactc ggttttctcg
     7081 ccgcgtttct tctgctggcc gggatggcgg cggcggtggc cattcctatc ggcaggtgcc
     7141 ggtgcgagta tcgtgggtga cagagcgcat attaccacct ccgtccatag gatcacccat
     7201 gaagatgctg atcaacggcg agtggcgtga ttcggtctcc gggaagatgt ttgcggtgca
     7261 taaccccgcg acaggggacg tggtcgggga agcaccgctc ggcggcgagg acgacgttcg
     7321 atcggccgta caggccgccg gtgaagcctt tggcgactgg tccgcgaaga gcccgaggga
     7381 ccgctcgaaa gtcctcttct tcgcagcaac ggaggtgcgc cgcaggaaca ccgatcttgc
     7441 ggcgctcctg actgccgagc agggaaagcc catccgtgag gcggtcgacg agatcaacgg
     7501 gttcgcaaac atcctcgagt actaccatgc cctctctgca ggggagcggg gggagttcat
     7561 gaatctcagg ggttatggcc gcaccacggt cctgagacgc ccgctcggca tctgcggcgc
     7621 gatcgtcccc tggaacatgc cggcgatcat catggcctgg aagatcggcc cggccctgac
     7681 ggcggggaac accgtgatct taaagcccgc ccggaccgcc ccgctgacct gcatcaaact
     7741 cgcggagatc ctcggggacg cagggcttcc accgggtgtt ttaaacgtcg tcaccggtcc
     7801 cggggagacg gtcggccggg agatcgcgag gaacccgggc gttcggaagg tctcgttcac
     7861 cggcgaggtc gggaccggga ggcaggtcgc cctggacgcc gccccggcgt taaagcggct
     7921 cacgctggaa ctcgggggaa gcgaccccat gatcgtctgc gacgatgccg atatcccgat
     7981 ggccgtcgag ggggtcatca ggggacggtt ctacaactgc gggcaggtct gcacggcggt
     8041 caaaaggctc tacgtctacg agtcgatcgc ggatgagttc gtccggcgcc tgacggcgag
     8101 ggtcgaggcg atcgtggtcg ggaacggcat ggaccgcggc gtcggcatgg gcccgctgaa
     8161 caaccctgcg ggccttgaac gggtcgtcag ccaggtcgac gccgcccggg accggggcga
     8221 gggggagatc gtcgcgggag ggcagactcc ggagggtgag gcgtacaaac acggtctttt
     8281 cttcctgccg acgctgatca ccggtgttcc gcgcgactcc gtcctcttct ccgaggaggt
     8341 ctttggcccg gtgctgccga tcgccaccgt ttccggcctc gacgaggcgc ttgatctcgc
     8401 gaacaactcc cgctacggtc tcggggcatc ggtgtggacc cacagcgcgg acgtgatcgc
     8461 ccgggcgacg gacgaactcg aggcggggat tgtctgggtc aaccagcacc tgaagattcc
     8521 tccggaggtg ccgtttggag ggacgaaggc gagcggcatc ggcagagaga acggcagccg
     8581 ggcgctcgag gagtatatgg aggagaaggc cgtgctgata cggctgtagg cccccgggct
     8641 ctcttcattc tccggtggcg cgggagatgg ggagcctttc gggaatgtga ggggtttgtg
     8701 gaccgtgcat cgtccggccg gtcagggtcg aggtctccgg tccatcacaa aatcggttga
     8761 agggggccgt tgcgcaatca ggattatctt actaattata gtaagattta tataattcaa
     8821 tacgtatcct tccccctccc aagaggggat ggtagtatga gacgatggat agccctatgc
     8881 acatgcattc tcgcccttct ggcgatgccg tttgctgtaa ctgcggcagt ggtgggagac
     8941 gagaatgcaa cgatgacacc cgggaaccag accgggcaga tggataatca gacagcaaat
     9001 atgacgattg ccgcctacat cgagcaggac cagaacctga cgaggctggc tgaggcaatc
     9061 agcgtggcag ggctctatga caccctgaac accggaggac catacacggt atttgccccg
     9121 agcgacgatg cgttcgacgc tctcgggaac gataccgtga accagctcat gaacgagacc
     9181 gataacctga ccatggtcct tcagtatcac gttgttgcgg gtgagtacac ctcggtggac
     9241 ctcatcaaca tgaccgagaa ccagaccgga aatcagaccg ggaatgagac cgaaggcggc
     9301 attctcgata tcttcagcgg gctcctcggt atggggaacg agaccgggaa catgacggcg
     9361 ctcgagacgc tcgctggtga ggacctgaac gtcaccgtca gcgacggcga ggtcatgatc
     9421 gagaacgcca ccgtcaccac ggcggacatc aacacaacga acggcgtgat ccacatcatc
     9481 gaccaggtgc tggtgtcccc gggcctgaac ctgacggcgg ctgagaacca gaccgcgatg
     9541 gaaaccgaga cgccgaccga gacgatggcc gggaaccaga ccctggtggg cgtcgtacag
     9601 tgatgtaaag caacagggcg aagggcggcg gcagggatcg ccgcctgccc ccggcaccgt
     9661 ccgggagact gcctgagcgg ttctggacgc tgatcccgct tctttttttc tggaataact
     9721 gagtatcctg caggtgttgc gcgccacccg ttacctgata ccttctgccg tgtccggatc
     9781 tcttcttcaa tcccggagaa tggtgtccgt cgtgatggtc atgaagagat aacctgaaac
     9841 cggaccggca tttatcaccc ggatctgccc catttggctc taaattaatt ttatgacaac
     9901 atatttatat taccttgatt attctcttgc ctgcaagggg ggatatgtat gagacgacct
     9961 gtactgttca gtttatgcat cgttctcctc ctgtcgatct ctgccgcaag cacggcaatc
    10021 tcgattggag ggggccctgt cgaactgaac cctgatgagt ttgtgaacat tacaccggta
    10081 aacagcaccg agacgtacga ggtgccgcag gcgacggtgc ttggagcact tgacgccgcc
    10141 tcgatcctcg gagtattcga gtaccaggtt gctgaagacc tgcctctgga ggaggggaac
    10201 ctcagcattc tatcgattgc aggcattgag aacgagatgg tgaatgagac ccagaacaac
    10261 tggacgtact gggtgaatgg tcagcagggc acgaccgggc ccgcggtgac gaacgtgact
    10321 gatggggaca atctgaccta ctcctatggg cccccgtcac ataccctgga gaacgcctcg
    10381 tatacgctga cggtgaacgt gagcgtgctc gaggcggcag tgaacgtgac gccgacnnac
    10441 ggtcaacgtg accccgggag cgaacgtgac gcctgccgtc aacgtctccg accagccgat
    10501 tgtgaacgct acggtgatcg ttgacaatgc cacgatcaac cggaccggct gggcggacat
    10561 ccatgcggac ctgaacggca cgcccggagc gatcatcggc tacagtccga tcgtcgaggg
    10621 cgtgaacgag aacgtgaccg tggagatcga ggtcgagaac gccacgccgg tgctctatgc
    10681 catgctccac gtcgatgagg gggagccggg tgtccatgag ttcccgggac ctgacatgcc
    10741 ggtactcctg aacgggagcc ccgtccagca ggctttcaac gtgaccggag ggctgccggg
    10801 agtgaannga cgccgacggt caacgtgacg ccgacgccga cacccggagt gaacatcacc
    10861 aatgagatcg ctgcagggct ttcagggcag gagaacctga cgacgttcgt cggtatcgta
    10921 aacaactcga ctctcggcga gaggcttgac gagaacagga gttacgtagt ctgtgctccg
    10981 accaacgatg cgtttgccgc ggtgggcaac gagacactct cggtgatcct gaacgacacg
    11041 acgctcctga acagcatcct tgattaccac gtcatccagg gagactacac gatcgaagaa
    11101 cttgtgatgc tctgccagaa ttcgaccgac ggccagatct ccctgccgac cgttgcggga
    11161 acgaatgtta acgtctcgct catcgacagt caactgatca tcaacgacat cgttgttata
    11221 acccagatcc agatcacgaa caacatcatt gtctacgtga tcgacggcgt tctgattcca
    11281 ccggaagtcc cggttccgac tccaacgccg acgccgacga tgaacgtgac ac
//
LOCUS       DAJB01000013            3193 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65972, whole genome shotgun sequence.
ACCESSION   DAJB01000013 DAJB01000000
VERSION     DAJB01000013.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65972"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    790..950
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          544 a          970 c          933 g          585 t
ORIGIN      
        1 cagccgctcg acgccgtcct ggatgtgctc gacggcaagg atccggatcg gcccgatctc
       61 gccggttgtc cggacatgcg ttcccgcaca cgcctgcaca tcgcttccca cctgcaccgt
      121 ccggatctcc cggcccggcg ggacaccgcc ctggtagagg ccgaacccgt gcttctgctc
      181 ggccttcgtc cgctcctcga tacgaatgta gaccggcgta tcggccatga ccagacggtt
      241 cgccccgatc tcgatcctct tgagttcttc gggcgtgata tgcttgtaat gccggatgtc
      301 caggcgggag gtctcgctcc ccttctgggc gccggcctgg tgcacatgaa cgccgagcac
      361 cttcctcgcg gcgtgcagga ggacgtgggt cgccgtgtgg tggcgcatca gcgaccaccg
      421 gcgttcctcg tccaccatgc ccttcacccg gtcgccacgc atgagggggc ctccggagac
      481 ccggtggagg atcacctccc cgatcttcgt cgcctcctcc acccgcacca tgctatcaga
      541 cgtcaccagg gtgccggtat cggaaggctg gccgcctccc tcggggtaga agagcgtctg
      601 gtccagaact accatcccgt caaagtagtc cagcaccatc gcctcgaact cgatttcgtt
      661 cgggagctca tagtagagct tctttgtcgg ggggagagtt ttcgcccgct cgcggtaggg
      721 agcaagcgga tccgcccctt tcgcctcctt ctgagcctcc gagtgcgtat cggcgatcag
      781 tgagtagaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accacatcga
      961 tatcctgccc gaagttcgcg gtccccacga cctgcatctg tgcctcgatc aggtcgccca
     1021 ggtcctcgtc catcgagagg tcgttcatca tccggagcgt ccggcgaagc acgagtcggg
     1081 cgagataccc ctcacggacg ttcgagggga cgatgcagtc gccgagcatg taggcgaggc
     1141 agcgggtgtg gtcggcgatg gagtagacct tctcgatcgg gacgacgatc cgctcaagcc
     1201 gctcgacggg tacgtcgatc gcctcggcga ccttcttccg gaggttgtag aggttcgtcc
     1261 cggagatgtc cataaccccg gcgaaccggg cgctgagccc catgatcttc gtgaactccg
     1321 ggttgtcgag gagatgctcg aggtgagacg aacgcatcag gcggttcacc atctccggaa
     1381 agaccgcatc gtagatcgtc ggcgacccct gcgaggccca gacgaacctc tcgaggccgt
     1441 agccggtatc cacgatattg agccgcatcg ggtagtaggg ctgcccgttc acatcgacgg
     1501 gcggcttatc gttcttctgc cgcccgaggt tcatgaagac gagcgtcgcg acctcaaggc
     1561 cgccgatgag cacctcgacg gatgccccgg cgtttccgcc gccgtaccag gggtgttcct
     1621 tataactgac ccggtcaagg tcgcccccga tcgacgcgat gaactcgtcg cagagggcga
     1681 ccgtctcttc cttccagtag atctgctctt cgggggtgtt gaaggcgtga tgcgccatca
     1741 tctcaaagag cgtcaggtgg cgccccgacc tgccgacgga gtcgagatcg tttaggcgga
     1801 tgcagggctg ggagatggtc agcgggttcg ccggcggggg gacgaccccg ctcgtgacga
     1861 atggctgaaa atcggcgata gatgcgatgg tgaggtagat atcgtctctc caccgggcag
     1921 ctacggggta tcgctcgagg cgtgtatgcc cgtgccgctc aaagaaggag aggaatgcct
     1981 cccgcatctc atcaacggta tgaggtctga aaaccgggtt gccgatgaag ttatacgtca
     2041 cacacggagc gtcgccgcag aactcttgtt cggggtcccg ggtccagaag gctgccccgc
     2101 aactcttgca gaccttccgc tcaaacccct gggaatggaa ataatcgagc gcatattctt
     2161 cttcgagcat tgaaaaccac gcagtatgat cagattcgac cgggaccgat atagttgttt
     2221 gtgcagattg tttatagttt ccaggtgtcc tggtaccaga gatcgtagga ttcgtataat
     2281 cccgtccgtt cggcgatgcg tacggcaaga atgtgccagc attcgcgtcc ccggaagagg
     2341 aagtcgctgc aggtgcagaa gtcaccttca acgatatact cgtcgttccg gcccaccacg
     2401 atgaagaagt cccggtagcg cttcacctgc cctgcatcga cggctcgaag tgcctttttt
     2461 ccccgctccc cgtaggcact ctcgatacag gcgcgaaact ccggtgtcag cgcccggtcc
     2521 cgctcgatct tctgccagat actgctcatg gaactaccac tacgtgtggc ggatcaggga
     2581 tataatgcca tcccatgcga gaggagagca cctccatggc gggggcctcg agcgcatagt
     2641 gggtggcctc aagacagggt atgggcgacg cccgggccac gctgtgcttc agctccgccg
     2701 agaggaaagc ttccgccccg agggcgacgg cctcccggat caggtcgggg tcaaacccgc
     2761 tcccccccac gaccgccagg cgccggatcg tccccgtctc cccgtagacc cggatccctc
     2821 cgcccgggag gcgccgggcg atctccttcg cgtcgagggt gcagtcgccg acgaggccgg
     2881 cggccatccg ctccgtcgcc aggagatcga gcctggatgc caggatgtcg ttgacgcctc
     2941 cgggggcatg gtcgaagttc gtgtgcatta catagaggtt catgcgggcg gcaaggagcg
     3001 tccggagcag gcgcgaggtc ggaccccgga tcgccgtgac cgggctccag aggggggtgt
     3061 ggtggacgac cagcatatcc gcaccggcgc gtactgcggc attcacaacc ctctccgtgg
     3121 tgtcgagcgc acagcagacc tcttcgatct cgcttctccc ctcgacgatg agaccgatcc
     3181 tgccttcatc ata
//
LOCUS       DAJB01000014            3752 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_119856, whole genome shotgun sequence.
ACCESSION   DAJB01000014 DAJB01000000
VERSION     DAJB01000014.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_119856"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          797 a         1044 c         1001 g          910 t
ORIGIN      
        1 agagggtatc gccaggaact atagttacga tagtccgtgc tggatgagga gtcatacgaa
       61 gggtttctgt tccatcagga gtgaagatag tctgattggc ataaggtctc acttatggga
      121 tgcgaaaaag gcttaatagt cggtcaggaa tctgatcctt ctctttcaac ggaggtatca
      181 tcttttttgg gaaattggga cagcgtggat tgattcctca tggcattctt ccggtagtcc
      241 tcaagcgcga gtttctctcc ctccgcgctg gctttctcat cgacccacct atcaataaat
      301 tttgcatcga tattctcaag aacagcacgg agtctggatc cccggcagtt gagccggatc
      361 gcatcggcat cgacttcatc gaatagttga tacttctgcc cgtattcgag gacaattcgt
      421 aacttctccg tcaccatctg cttcttctga tcttctgaaa cgcccaggga gacgttattc
      481 atcaaggtcg agagcactgc gtttttctgg atgatcttcg gatcttcgat accatcttcg
      541 aacaggttct ttcggtagca ttccccgata attgccagct ggatgagccg gtaagggttc
      601 gtcgaataac agtaagggag ttcatactgc tggtggaggt cctgatcgcc gaaacccgcc
      661 tgcagcatct catttaatgc cgaattctca tgcgggcatt cgaggagaag ctgtatgagt
      721 tgagaccgcg gggcgtaagc ccagactttt atcaggttga tatcgtattt gggatgcggc
      781 ttctgaccct gtgccttcat ggactcgatg atcttcatct tgtccttctc gttgatgcag
      841 agtacgtact cgaaccggtt gggatcaagc tcgatgtcta tggcatctga aaggtagtcc
      901 gtcttttcaa ggaccagatc acgggataat ccgacctctt ttaacgtctt tcttacatcg
      961 gtgatcgagg atttgcactc gacgaagatc gcacggtttt caccttcgtt gtagaggaag
     1021 agatcgaaag tgtgatttcc cttttttgcg gagccgaact cgatcagggg ctctgcaaaa
     1081 taaaattcgt atccggatct ctgtgaaagg tcggtctgtg cgaaagattt tgcgcattgt
     1141 ttcacgagag tgtggtgctt tttgtcttcc tcaagagcat caccgatccg ctctcgtgcc
     1201 gagggtatgg agtaaatttt atatttgtcc tttaatttat ccagatcctc gcagacttca
     1261 gatggagaac gcttgtagaa cgcctgaaaa tcttccttct cacgaggaat atggatggta
     1321 tcaccctacg gtcgccatat gatcgatatt ctcgaccaca aactcgaaaa acctcatgga
     1381 tgttccgatg tctattttct ctaccgggta gacgtcgatc ttcttgccgg tagtcgagat
     1441 gtccaggaga gaacctgtgt gctcatcgat caaccgggcg gagaagaaca ttgaacccgg
     1501 cagcagcacc tgctccagta cggtaaagtt ccattcctcg ctgcgcatcg ccttgcagaa
     1561 gcgggaaacg tcatcgtaat gcatctccac cggcacatcg attgaccagg ggatctgaat
     1621 ggcattcgag aattgcctgc gggacccgac cctgctgatg gaatatcccg actctccgat
     1681 tgcctctctc acgttttcaa gccgtgaaac gacttcctcg attatcttga agatgccggc
     1741 gaagtgtccg tgacgaagtg taattatacc cttattgtcg atgcggaatg caatgccgtt
     1801 cgggagctgg acctcaagaa tctttggcaa cacaccgtag tagaattcca tctcctcaag
     1861 cgcgtgtttt ccgtcgttcc ccctgtactg gatgcctctc tcgacatgag gcctgaactc
     1921 ggagtgaata tcggtgttgg ggctcctcct cgcagagaaa taggttataa tcatgtccgg
     1981 gtattcccgg acgaggttat ctttgatctc cttcatcacc ttaggggcta tccagagatt
     2041 cgatatctcc tttgtacggt tgatgtaagt gaagaggatg tcaggtatct cattggtttt
     2101 ctttgcatcc gtgatgaaga gcgggacatt gttcctgtcg tcgaagaacg tataaatata
     2161 aatccgttta ccctgggatt ccttcgagag gaggagcagt tttcctgccc tcttatcgat
     2221 ggagaaatgg aagcttttga ggctctcgat gaactggtcg atgttttgga acatcgacag
     2281 tgttgcaact atgtggagat tactgttgat ctggtcgtct cctcgctcac attcaaggta
     2341 ctggatcagc tcctgaatgt cgtcgaagtc tctatcagct ctgatgagcg aagggaggtt
     2401 caaagtatca catccccggt taatttccat taagtttcat gtagatttag atgtttctta
     2461 tttgaacctg atgatatagg cggaacgtga aggtatcttg aggattgcca aatatcaagt
     2521 caatctgaaa ataagatgga ccgagcggga attgaacccg cggcctcttc catgccaagg
     2581 aagcgatcta cccctgatct atcggcccct gttaccctac aatgttgatc gtgatcacat
     2641 aataaggatt ggggccggcc gctccccgat cactccccgg cgaggtccgg gagccgggat
     2701 aacacccgtt cccgcgcatc ctccatgttc ggccgcgcga cgatgatgcc gtgctcgacg
     2761 tagcgggaga ggagcggcgt cgcaccctcc ggcgccggag cggtgagggg gagcgtgacg
     2821 cgccgccccc cggccgcttc atagacctgc tttgcgccgc tccgcttccc gcgcttcgca
     2881 tacggccgcc cttccatctc gacgatgtcc atcgaaaagt cgatcaccgg ggcgtttgcg
     2941 atcgcccccc cgatgccgaa agcgtcggcg atatcgcggt aggcgacgac gtctgcgcgc
     3001 gacaggccgc ccgagaggaa gatcttcacg tcggagtagc cgtggacgtc cagttcccag
     3061 cgcacctcct cgacgatcgt ccgcatatcg ccccggcgcg accgcggcgt atccagccgg
     3121 acggccgtcg ccccgcatgc cgccgccctg accgcctcgt ccttctcgtc ggagaaggtg
     3181 tcggcgagca tgatccgcgg cacctccggc cctgcccctc gcgcaaacgc acaccaggca
     3241 tcctcctgcc gcgggtagca catcacgaac gcgtgcggca tcgtccccgc aagcgggacc
     3301 ccttccggcg cacaggtgct gctcgcgccg tccaccccgc cgatccaggc cgcccgctcg
     3361 atcatcgcag agatcgccgg gtgctggcgg cgggagccga aggagtagac cgggcggtcc
     3421 cgtgcagcga gccggatgtg ggcggccgcg gacgcgaccc ccgaagcatg gcagaggaac
     3481 ccgaggatgg cggtctcgta gaccgcaaag tcccggtagc gccccgacac ccggaggacg
     3541 ggctcgtttg ccgagaagac cgaaccctcc ggcatcgcgt ccacgtccac cgggagccgc
     3601 tcaagcagcc ggacaacatc atcgaggccg cataagactc cccaccggtc cgggagcgcc
     3661 gcagccgtca cctccatcgt gacgtgcggg ttgaccccgt ccttctccat gacgtccaca
     3721 acccgcttaa agtagatatc ggtgcatctc cc
//
LOCUS       DAJB01000015            8855 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_104884, whole genome shotgun sequence.
ACCESSION   DAJB01000015 DAJB01000000
VERSION     DAJB01000015.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_104884"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1742 a         2627 c         2897 g         1589 t
ORIGIN      
        1 gatccccagc tgttccggaa gaccgtcgga gcggtgcctc tccgggacga gtacatccgc
       61 tcgcttattc tgttcttcct tgccattcac gatattggaa agttttccga acggttccag
      121 ggcatcaacc ccgacctcca gtcgcgcctc cagggcgctg tctgccgtat gccgtatagc
      181 caccaccaca cgacgatggg catgctcgtc ttcatgcggg aagtgtggcc gctggtacgg
      241 gacgagagat ggttgggaac agaccccgac gaggatccat gtgatctgga ggatctcttc
      301 ctcccctgga tccgggcggt gaccggccat cacggaaagc ccgtcaccga cagaggcaca
      361 ttccccgacc tcttcagccc gatggaccga aaggcagcgt gcgagttcgc ccgggcctgc
      421 tcggccctgt ttctctcgga agatccggaa gagccggtcc agtattcaac cgaactgtct
      481 gagtccttca cccgcatctc ctggctgatc gccggactgg ctgttgtgag cgactggata
      541 ggatcggacg ccgagatctt ccggtattgc caggacccga tgccccttga ggagtactgg
      601 cagcgctacg ccgtcccgag ggcgaaagag gcggttgccc gggcaggcgt cctgccgtcc
      661 gccatcgccc ccttcccggg gatagcgtcc cttcttccgg aaaacgcggt cccgacgccc
      721 ctgcaggaag ccgtcgccga ctacgagctc gccgaaggcc cctacctctt catcgttgag
      781 gacgtcaccg ggagcggaaa gaccgaggcc gcgctcgcac tgacacaccg cctcatggag
      841 cgcgggtctg ccgaaggcat cttcatggcg ctgccgacga tggcgaccgc gaacggcatg
      901 taccggcgat tcggggagaa ataccgacaa ctcttccagg atggcgagca cccctcgctt
      961 ctgctcgccc acagcgcgag gaacctttcc aatctctggc gccagtccat cggcccgcaa
     1021 accgataaaa cgactttcac gccaggggga gaagagggat cggctgagtg cgcagcctgg
     1081 ctctcggaca accggaagaa agccctcctc gccgatgtcg gtgtcggcac catagaccag
     1141 gcgctcatgg cggtgctacc gctccaccac cagtcgcttc gcctcctggg ccttgcccgg
     1201 catgtgctcg tcgtggacga ggtgcacgcc tacgacgagt acatgaacac ccttatcgag
     1261 cggctgctgc agttccatgc ggcttttggc ggcagcgcaa tcctgctctc ggcgacgctc
     1321 ccgatcagac tccgggaacg gttcgttgcc gcttactgct ccggcctccg ggtcccggca
     1381 acgccgatac acgaggccga ccctcctctc atgaccatag tttctccccg gggcgtagaa
     1441 gagaggccga tgcaggccga tcgtctttcg cggagaaccg ttgcggttga actcaccagc
     1501 gatgaagccg atgtagagtc ctacctcgaa cgggctcttc tggaaggccg gtgcgcctgc
     1561 tggatccgga ataccgtcat cgatgcgatc gaggcataca accgcctcgc cgcgcgatgg
     1621 ggcgacgaac acgtcaaact cttccacgcc aggttcaccg ttggagaccg gcagaagatc
     1681 gaggacgatg tgctgacatg gttcgggaag cagagcacgg atgccgaccg cagagggaag
     1741 atactgatag caacgcaggt tgtcgagcag tcgctggata tcgacttcga tgcgatggtt
     1801 acggaccttg cgcccatcga cctgatcatc cagcgggccg ggagactgca ccggcaccgc
     1861 gagagaagga gcgggcttgt tgtaatgccc accctgctgg tcctctctcc ccctccggtt
     1921 gaggctccag caaaagattg gtttacgagt gtcttccccg gagccgggcg ggtatacgag
     1981 aaacacgggc aactctggct caccgctcgc ctccttgccg cccggggacg gatcgtgatg
     2041 cccgacgatg cgcggctgct gatcgaaggc gtgtttggag aggaagcgca ggcatccgtt
     2101 cccgaggcgc tcaaggtatg ggagatacag gcagacggaa aagaccgcgg gcatgcggcg
     2161 ctggcgcaga taaacgcact gcggccgcag gcagggtatg tggacctgca cgggcagtgg
     2221 gaggatgaga gccggacacc gacccggctc ggtgaagcga gcgtcaccgt cgtgctggcc
     2281 cgccaggacg gggccgaact ccggccctgg aatgaaagcg gagaggccgc ctggggaaga
     2341 agccaggtct cggtgagaga gtcccggata gcaggcccgg cacactgctc cggggctgaa
     2401 gagcgtgaga tcgcacgggt aaaaccgctc ctgcccggca ggggcgaagg aagcatcctg
     2461 atccctctga agcccactgc agacgggttg tgggagggac gcgcacagga tctgaaggaa
     2521 aaagaggtga tcgtcaccta cgatccgaag atcggcctgc agatccggga tggctgatca
     2581 tcatgtgccg tgaaatgaat ccgattttac atttatccct tattttccct gataacgcct
     2641 gaagggttca ccagaacggt cagatggaaa agtataaggt atcatacaac aagcattcac
     2701 cagacattgc cgtactcgtc tcttccggtc gcatgcgaag atcgctccat tgactggtgc
     2761 agagcggtca ggcaatgcag gtgaacagac atggtcaatc tcatcgatga tccatggata
     2821 cccgtcgtgc gaagggacgg agcacgggaa accattgccc cccgggagat taccgggggg
     2881 gccgagcccg tgatccggct ggacgcgccc cgacccgatt tcaacggggc gctgatccag
     2941 ttcctcatcg gccttgtgca gacggcaatt cctcccggtg acaaccggga ctggaggagg
     3001 aagtttaaga ctcccccacc tcccgatgaa ctgaaacgtg cttttgcacc gtatgcccat
     3061 gctttcaact tcgatggcga agggccgcgg tttatgcagg actatgacct caacgagggc
     3121 gtcgagagct ccgtagatcg gttgctcatg gagatgcccg gagagcagac gctgaaactg
     3181 aataccgacc attttctgaa gcgaggaacg gtcagccgga tctgccgccc ctgctgcgca
     3241 acggcactct tcacgctcca gacaaacgct ccatccggtg gccgggggca ccggacgtcg
     3301 ctccggggcg gagggccgct gaccacgctg gtcatgggca cgaccctgtg ggagaccgtc
     3361 tggttaaacg tccttccccg ggaccagttc gagatcctcg gcaacgccgc aaaaaccgca
     3421 gaccccgacc ggttcccctg gatggggccg acccgcacaa gcaccagaaa cgaggttacg
     3481 gctcctaccg acgtgcaccc ggcacagatc ttcttttcca tgccccgcag gatccgcctc
     3541 gacctggatc acccggagtc gggaaggtgc gatatctgcg gttgtgcttc tgaggatctt
     3601 gtctcgcaat acgccacgaa ggatggcggg gtgaactaca aaggggcctg gaaacacccg
     3661 ctcacgccgt attaccagcc gaagaacgcc gaagagcctc tcccgaagca cgggcaggcg
     3721 gacgggatat cgtaccggaa ctggctcgga cttgtgcaga acgataccga aagccgtacg
     3781 cagcctgcgc tggcggtcca ggtcttccgg aacgagcggc agcgcaacct gaccgattat
     3841 ctgggttatt cggccccgct ctgggcattc ggttacaaga cggacaacat gaaggtggtc
     3901 tgctggaacg agagcacgat gccgctgctc catgtcggcg atgcggttcg tgagcagtac
     3961 gaaggggttg tgacggcact gatcaagact gccgatctcg tcgcagggag cacccgcagt
     4021 tgcatccgga aagcactctt cggagaccga aaggacgcag gcgggaatat ccccgaggtc
     4081 gattcccggt tctggcagga taccgagagc gagttctacg ctgtgctgga gaggttgcgg
     4141 gaggcgacgg agaagggtga ggatacaacc gccctgaaac tgcagtggct ttcgagcctc
     4201 tcccgtgagg ccgaaggact tttcaaggtc tattctcagg ccgatctcat cggtgtcgcg
     4261 gacccgcgaa ggatagccct gtcccggcgc atgctcagga attacacctc accgagaaac
     4321 aagaagatcc gcgaggcgct cgacctcccc cgggatcctg acgacagtga tgcaaagccc
     4381 aggggaaccc gaaagcgctc cgtacgtcaa ggtgaacacc catgaaacag atgcagtata
     4441 tttcgttcat gaacaacccg gacgcgagat cggtcctgat ggactggtgg gcgggtctgg
     4501 atcatgcccg tggagatcgg gcggcgctcc ggcgttgccg gaacagccag gaggtcgcgt
     4561 ttgtcccggc gttccaccgg ctgcgccaga acctctccag gattgccccc gtggatgctg
     4621 aaaaacttgc agtggtggcc ggggttctct cgcatgtcag ggagaacgac cactcgctcc
     4681 ggttcgccca gcagatggca acaccgaagg acggcagtga ccgtgcccgg gtgagcgggc
     4741 tgcgtttccg gcggctgctc aagatcgagg accgcgacga cctgtatggt gcggtggtcc
     4801 ggacaatccg gctgcttggc ggctccgtga acattgcgag ccttgcagac ggagtctact
     4861 ggtggaatga gaggacgaag aacacctggg cgtttgacta ctatgacaaa gccccgaatg
     4921 aagcgtaatt aatggaggag taaccatgag tgaatttatt cagttacaca tgctcgtttc
     4981 gtatccgccg tcgaacctga accgtgacga tctcggcaga cccaagaccg ccatgatggg
     5041 cggcgcccag cgcctgcgcg tctcgtcgca gagcctgaag agggcctgga ggacgtcgga
     5101 tatctttact gacgcacttg ccgggcacct cggcatcagg acgaaggaga tggggacgca
     5161 gatccaccgg tccctgacat cgggcgtcag ccttgcaggg atcctttcgg gcgtcaaaga
     5221 tgcacctgtc acattcaagc ctgtcgccga ggataaagcc cggaagtggg ccgcggagat
     5281 cgccggcggc tttgggaaac tgaaagccgg tgagggcgcc ctcgagatcg agcagctcgc
     5341 ccacttcagc ccggaagaga tcgccgctat cgacgcgctg atcgcaaacc tggcagccac
     5401 ggggagcgac cccggcgaca acgataagaa actcctgatg gagcggcaca ccgcggcaga
     5461 tatcgccatg ttcggccgta tgctggcagc atccccgatc tacaacacgg aggcggcggt
     5521 gcaggtctcc cacgcggtca cggtacatgc ggtcgcagtc gaggacgact acttcacggc
     5581 ggtcgacgac ctcaaccggg gcgatgagga tatgggtgcc ggacacctcg gggagacgga
     5641 gttcgcgtca ggcctctttt acctctacgt ctgtgtcaac cgcgatctgt tgcgcgagaa
     5701 cctgggcggc gacgaagcgc tcgccggggc tgctctccgc gccctcgtcg aagcggcggc
     5761 aaaggttgcg ccgaccggca agcagaacag ttttgcgtca cgggcatacg cgtcgtacct
     5821 gcttgcagag aaaggatgcc agcagccccg ctcgctctcg gtcgcgtacc tgagaccgtt
     5881 gcaggagaat gacctgctcg ggagcgctat cggagcgctg aaggagaccc gcgataagat
     5941 ggatgccgta tacgggaact gcagcgacgc ccatcgtgag atgaacgctt acgccggcga
     6001 aggaacgctg caagaggtcc tggatttcgt ggctggtcaa catgcatgag ttcctcgtct
     6061 ttcgcctgta cggcatgatg gcatcgtggg gcgatattgc ggtcggggag tcgcgcccga
     6121 cgttcgatca cccgtcccgg tccgcaatct tcgggctgct cgcggcggcg ctggggatcc
     6181 gccgtgacga ggaggagcgg ctttccgcgc tctcgaacgc ctaccggatg gcggttcggg
     6241 tggatgcccc cggggtgctg ctgcgggact accacacgtc tcaggttccg cccgcaggga
     6301 ggggaagaaa gaagcactcg ttttccaccc ggcgggagga actggcgata ccccgcgagg
     6361 atctctcgac ggtgctctcc acccgggatt accggtgcga tgccgtgtcg acggtctgca
     6421 tctggaaaga ttcggcgtcg gctccctgct cgctgaaaga gctggcggac gccctgaacc
     6481 acccggtgtt cgtcctgtat ctgggcagga agtcctgccc gcttgctctc ccggtgcatg
     6541 cacaggtcgt tgccggcgag acccttgcgg ccgcgcttgc ggcggtgcgg tttcccgatg
     6601 acgacctgct gcgggacctg ctccgccacg gcgacctgcg ggtcttctgg gaaggcgatc
     6661 aggacgcagg gatcccggcg gtccagacga ctctgcggta tgataccccg ttgagccgga
     6721 agcggtggca gttcgggagc cgggccgagc attacggcat tatgcggagc ggatcgccat
     6781 gttcctgagc aggatacggc tccggcctga cgcggccggg aaacgtgagt tctgggaggc
     6841 gatgggaagc gagtaccagg cgcaccatat ggtctgggac ctcttcaccg atagccccga
     6901 ccgggagcgg gactttctct accgggtgga ggaggccgag gggttgccca cgatctactc
     6961 ggtctgcgaa cgtgagccgg tgaaccgggg cgggatgtgg acgatacaga cgaaactcta
     7021 tgatcctgtg ctccgctccg ggcagcagct tgcgttcgtg ctccgtgcca atcccgtccg
     7081 gacgaagtgc gatgagaagg gaaagcacca ccgccacgat gtggtgatgg aggcaaagac
     7141 cctgttaaaa cagcagggca gaccccggga ggaatggccg ccggagccgg agatcgtcca
     7201 gcaggcaggg tttgcatggc ttgcgatgaa gggcgaggcg aacgggtttt cggtggcggc
     7261 gggcgatgtc cgtgctgacg gctatattca gcggcggttc cggaaacgca agggacggca
     7321 cgatatcagc ctcagcacga tcgactatac gggcatcctc acggttgtcg acccggagcg
     7381 gttcaaggcc gtcctgtacg atgggatagg gcctgcgaaa gggtttggat gcgggctgat
     7441 gatggtgcgg ccggcagtgc ggtagggagg acgtatgctc cctccgctca agccgattgc
     7501 catcaaggaa cggatctctc tcctttttct cgagaagggc gagctggacg tgcttgacgg
     7561 cgcctttgtg ctggtggaca agaacggggt gcgcatccag atcccggtgg ggagcgtcgc
     7621 ctgcctgatg ctcgagccgg ggacccgggt ctcgcacgcg gcggtggtgc tcgccgcccg
     7681 ggtcggttgt ctgctggcct gggtcgggga ggcgggggtg cgcctctatg ctgccgggca
     7741 gcccgggggc gcgaggtccg accgcctgct ctaccaggca aagctcgcgc tcgacgagga
     7801 ggcgcggctg aaggtcgtgc ggaagatgta cgaactccgg ttccagggcc ccgtcccctc
     7861 ccactactcg gtgatgcagt tgcgggggat ggagggggcc cgggtccggg agctctacgt
     7921 gcagctcgcg aggaaatact tgcttgagtg gaaggggaga aactacgact cttccagctg
     7981 ggggagcggc gacctcccga accgctgcct gagttccgcg acggcctgcc tctacgggat
     8041 ctgcgaggcg gcggtgctcg ctgccgggta tgcgcctgcc atcgggttta tccattcagg
     8101 aaagccgctt tcgttcgtct acgatatcgc cgacctcttc aagttcgata cggtggtgcc
     8161 ggtggcgttc cgggtcgccg cggagaaccc gttcgatccg gaacgagccg tgcggctggc
     8221 atgccgggac agtttccgcg agacgaggct gctcaaaaag atcatcccca caatcgagga
     8281 agttctgaag gcaggcggga tctccatgcc agaaccgccc gaggacgcac tgaaaccggc
     8341 gatcccggaa gagcgggggt tgagcgatgc tggtcatcgt ggttgaggcc gctccgcccc
     8401 ggctcagggg caggctggcg gtctggctgc tggaggtcca cgccggggtg tacatcgggg
     8461 acctctcgat gaaggtcagg gagatgatct ggcggcaggt cgtagccggg atcgaggatg
     8521 gcagcgcggt gatggcctgg agcatcaata ccgagcaggg atacgacttc acgacgatcg
     8581 ggaaaaaccg ccgcgtcccg ctggaactgg acggcgtgaa gttgatatcg tttctccccc
     8641 tgcaagggag agattccgcc ggtgttatca ccgatgacag cgaagaggat gatatcgaat
     8701 gtggttcgct cgatacttcg gagtgaggca gatgatggct ttaagcgatt cccagattcc
     8761 tcatgacgaa cctctgatcc tgtgtttgga tgggtggaag taatcttcca cctttctgga
     8821 ataaaaaggg atgagcgttc cccatgcacg tgggg
//
LOCUS       DAJB01000016            2644 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_127060, whole genome shotgun sequence.
ACCESSION   DAJB01000016 DAJB01000000
VERSION     DAJB01000016.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_127060"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    566..596
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1059..1092
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          467 a          824 c          779 g          507 t
ORIGIN      
        1 cagttgtttc gtagggcact accgcaaggc cgttcccgaa cctccccgga caaaagatgt
       61 tggccttacc gggatcactc cggaaccgga gcgatctgct gaaattgtta ccggcaaaga
      121 tgcttctgcc ccggcctatg acgattgcgc acgggttgag gggtgaggag tccggatcag
      181 gcacgccgcc gcttcacttc gtccaggagc tcgcggcgga ttccctcgat acgctcgttc
      241 accatccggg cgagaggttc ttccccggaa acatcataat acgaagtctc ccggcgccgc
      301 acctcttcga gcgtcctggc cttcgtcagt tccccgatga cggactgggc catattcgag
      361 tactcgccgc tgtcgacctt ctccttcagc caccggtaga ggtggcccgg cagccgcaca
      421 cccacctgct tgcttgaaac gttatcggaa gactccatct tcccattcag gtatcccggc
      481 agaacaatta tattatacca tttttatttt tccaacgaga tacctatgga taccaatcgg
      541 tatcattaaa cggtcggggc acccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggg
      601 ccagaaggca acgcgcccat caggagacgg tggtgatacc ggagaggacg cacctgccca
      661 ctgcccattc tgcgggtgcc gggaccgcat caaggacatc atttccgcca acccgcggat
      721 tcataccact caccaatgcc tccggtgcgg ccgctggttc gacgacctga agcgggcatg
      781 cccccggtgc ggatccccgg gggaactcct ggggtatgtc gatgcccccg gccacggcct
      841 ttatgcacaa taccgctgct gcgaatgcgg ccacgggttc atggtgcgaa tgaatcgcga
      901 tggagatgcc cctcagacct gacggcggat cggtcccggc ctctccgttt cttcccaccc
      961 cggatcttgc gcaggggttt gtgctgcggt gaaaaaagag gtggcgattt acgtcccgat
     1021 cgtccagctg ctcatgtagg tctcctgctc gggcgtcann nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnatggggtg gcccatcccc ttcggcgtcg caaggttcac gagccttcct
     1141 tcggcgagga cgtggacggc cttcccgcca agcaggtagg tgtcgatgcc gtcgcggcgg
     1201 acggtcgagt ccgcgtgcga tgcgagccat tcgacatcga tctccacgtt gaagtggccg
     1261 gcgttcgcga ggatggtccc gtccttcagg ttcgggaagt gccgctccgt gatgatgctc
     1321 gcattgccgg tggtggtgac gaagatgtcg ccgagcggca ccgccaggtc catcgtcatg
     1381 acatcgaacc catccatgtg cgcctgcagc gcccttctcg ggtcgacctc ggtcacgatg
     1441 acgcgggcgc cgaggctccg ggccttctgg gcaagccccc ggccgcagta gccgtaaccg
     1501 gcgacgacga accnntttgc cggcgatgag gacgttcgtc gtgatcatga tcgatgcaag
     1561 cgcactctcc ccggtgccgt ggacgttgtc gaagaagtgc ttcatcgggg tatcgttgac
     1621 ggcgacgacc gggaacttga gcgccccttc ccgtgccatc gcgcggagcc ggtggatgcc
     1681 ggtcgtggtc tcctcgcacc cgccgacgac cgagtccagc acctcctgcc gctcggtgtg
     1741 gatgcggtgg atcaggtcca tgccgtcgtc gatggtgatc gcagggcggg cgtcgagcac
     1801 ccggtcgatt gccgcgtagt actcctcgac gccggccccc cgccgggcat aggagtgaat
     1861 cccttcccgg gtgttgagcg ccatcgatac gtcgtcctgg gtcgagaggg ggttgcaccc
     1921 cgtgatgtgc acctcagcac cgcctgccgc cagggtctcg accagcaccg cagtctttgc
     1981 ctcgacatgg agggccatac cgatagtcat gcctgcaaac ggcttctcct cggcaaaccg
     2041 cttccgaatg gacgagagta ccggcatgta ctgccgcgcc cacgcaattt tgagatctcc
     2101 agactccata ataccaccag accggtttcc tgcccggaaa ttcccccggt ttgtctgtag
     2161 aggtatcttt caggattact taaatatgag ggatttgtgg ggtttggggt gcgactgttg
     2221 ggaggactgg agcatgagaa accctcatca tcttccgccc caaaaaccct actcatggtt
     2281 ctgacgaaac ggatcatccc ctgcctggac ctcaaagacg ggcgggtagt caagggcacg
     2341 cacttcgtcg gtctgcgcga cgcgggcgat cccgtcgcgc tggcccagcg ctacaacgag
     2401 cagggtgcgg atgaggtggt cttcctcgat atcaccgcca ccagggagaa ccggggcacc
     2461 atcatcgacg tggtgcagcg ggcggccgac gaactcttcc ttccgctcac cgtcggcggc
     2521 ggcatccgga cgctcgacga catgaagcag atcctccggg cgggtgcgga caaagtgagc
     2581 atcaactcaa gcgccgtcca ggacccggaa ctgatctcgc ggggcgcaga gcggttcggc
     2641 accc
//
LOCUS       DAJB01000017           21827 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_45737, whole genome shotgun sequence.
ACCESSION   DAJB01000017 DAJB01000000
VERSION     DAJB01000017.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21827)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21827)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21827
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_45737"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3683 a         7211 c         6988 g         3934 t
ORIGIN      
        1 agacccgcac aatccgcctg accgctttct cgaggttctc catcgaggtc gcgtaggaga
       61 gccggagcca gccgggggtg taaaacgcgg ctcccggggt gaccgccaca tgggcgtcct
      121 ggagccacga gcgggcgatc gccatgtcgt cgccctcgac cttcacgtag gcgtagaagg
      181 cgccgtccgc gggagccgtg gcgtagccga ggtcacggag cgccggaatg aggtaatcgc
      241 gccggcgctc gaactcgcgg cgcatcgctt cgacgcagtc ctggctgccc tggagggctg
      301 cgacggcgcc aaacatcgca aacgtcgtcg ggtgcgatat ggtgtgctgc tgcaccttct
      361 ccatctgccg gatgactgcc cgcggggcga ccgcgtagcc gatccgccac ccggtcatcg
      421 cgtaggcctt cgagaacccg ttgatggtga tcgtccgctc cgccataccg tcgagggagg
      481 cgagagagat gtgctccttc ccgtagacca gcttctcgta gatctcgtca gagagcgcga
      541 agaggtcgtg gtcccggcag atgtcggcaa tcaggcggag tgagtccgcg ttcagcaccg
      601 cgccggaggg gttcgagggg gagttgacca cgatcatctt cgtcctgggg ccgacagtct
      661 cgagcagggt atcgtcgacc tggaaggtcg cgggagagag cgcgtggtgc cggacgctcg
      721 cgcccgtaaa ccgggcgcac ggttcgtagg agacccacgc cggatcgagg acgagcacct
      781 cgtccccggg atccagcacg gcctccatcg cctcgtagat ggcatccttc gcgccgcagg
      841 tgacgatcac ctcatcctgc tgtgcgttaa agccgttctc ccgggtgatc ttctccgcga
      901 ttgcgcccgt cagtgccggt atcccggcgc tcggggcgta atgagtctcc ccgcggcgga
      961 gggcttcgat gcaggcactc ttgatatgtt ccggagtatc gaagtccggc tctcctatag
     1021 agaggctgat gacgtcgata ccctcctgta gcatccgctt tgccgcgttc gagatctcga
     1081 tcgtcgcgga gggggcgatg cccgcaatct tctccgagag gcttctcatc ccaatcgctg
     1141 gaccatcttc acggccgact caacggcacg cttggcgtag tcgatacgct cggtcgcttc
     1201 catccgggtc atccccggtc ccgagatgcc gagggagacg ggcttaccgt attcaaggga
     1261 gaggtcgatg atcttccttg cggcatgctg gacgacgatc tcgtcgtgct gggtggcgcc
     1321 ctcgatgacg cacccgatgg tgacgaccgc gtcgacgttt ccgtcgttga gcatcttctt
     1381 gatggcaagc ggcatatcgt aggcgccggg gacgtagaac gtctcggtca cctcagcccc
     1441 gaggaaaccg gcgtgctccc gggcctcgat ctccatcatg taggtgatgt cgcggttgaa
     1501 ctccgcgacg acaaatccaa gttttaccgt cattccaatc cttcctgtgg tactactcgc
     1561 accggttgtt gataatcctc acttttttcg tcaggggcgt gccggcccga cgtcctcgaa
     1621 cccctgccgc tggccggtcc cggcgagttt ctcgaggtcc ttcgggcgga gcgcgaggag
     1681 gacggcgttc gccgcgtgct ccctcgtccg ctgctccatc agccaggcga gttcctttgc
     1741 gtccttcgcc tcgtcctcgt ggacgaagac ctcgatgatg tgccggttgg tcatgagctg
     1801 gcagaggatg aggcccgaag aggcctcgtg ggcgcagact ttgtccttct cggcacgccc
     1861 gggcatcccg agcgccatca cgaggtcgca cccccgctcc tcgatcagct tcttgcacgc
     1921 caccgggagg tccttgatcc cggggacgac gtagcgctcc agccccacgc tcgcgtgctt
     1981 gcggatctcg tcgatggcga tcctgcccat gtccacccgg gcaaacgtgg tgtcggcgat
     2041 cccgatcttc atccgagaag caccccggcg gctgcctcaa cgccgtcgcc ggcctcaaac
     2101 ccgaacttcc tgagggtgaa ctggacggcc gcgagggtgg cgaggatctc ctgcgccccg
     2161 acgccgccca tggtgccgat ccggaagatc ctgcccttga ggtggtcctg gccgccggca
     2221 atctcgatgc cgaactgctt gaccgttccc cgcaggtcct tgtcggtgat gccatcgggg
     2281 atccgcatgg cggtggcggt gttcgagtag gcgtggtgtg cgtccagtgt cgggaagagg
     2341 tcgatgcccc atccctttgc tgcggcgcgg acggagtccg ccatccggcg gtggcgggcg
     2401 atccgggcag gaacgccctc ctcctcgatc attttgcacg cctcgtgcag cgcgaggaag
     2461 agcgggaccg ccggggtata gggggtctcc atcggggtgc cgctcccact cttcctgtag
     2521 gcggccatat cgaggtagaa cgggcgcttc tcggatatcc ggtcccaggc acggtcgccg
     2581 accgcgatag cggcgaggcc cgccggggcg gcgaggcact tctgcgatcc gacgacggcg
     2641 atatcaaccc cccattggtc catccggacg tcgtcgccgc cgatggaagt gattccatcc
     2701 atgatgaaga gcgcgtcgtg cttccgggca agttttccaa cctcgggcgc ggggttcttg
     2761 atcccggcgc tcgtctcgtt gtggaccatg gtaacgacct cggccccggc ttcgagctca
     2821 cgctcgagcg ccgcgaggtc gagcggcgtc ccccactctg attcaagggt ggcgaccata
     2881 ccgtaacgct cgccgatctt cgcgaagcgt tcgccgaact tgccgttcac gagcgagacg
     2941 atctttctgt cccgcgcgaa gttggcgatg ccggcttcca tgccggcgct tcccgagccg
     3001 ctgatgatgt agagttcatt ctcagtaccg aagagggtct ttaatacccg ggtgcagtcc
     3061 gcatacgcag ccccgaactc gggaccgcgg tggttgatgg cctgccgcgt catcgcggcg
     3121 cgtacccgtt gcgggacggg tacagggccc gggatcataa ggagtggttc gtattccatg
     3181 agagatcagt ccatattgtc tcgtcgagga gagatgctgt tcctgcaagg aacttggatg
     3241 tggattcata tcaacctctc caccacgcgg tatcgacaga gagatgagga ggcaggggca
     3301 tattgaaggg aggtatggtt gacgtcgcgg ttatctgcgg ttccgcctcg gacggccccg
     3361 ttgcggagag ggtgtttgcg gtcttaaagg agcacgatgt atcctacgac taccgggtga
     3421 tctcggcgca ccgtgacccc gacaaactgg acgagtacgt gaaagggagc actgcccggg
     3481 tcttcatcgc gatcgccgga ctctcggcgg cgctccccgg agtggtcgcc tcgaagacag
     3541 agcggccggt gatcggggtc ccggtgagcg ggaaactgat gggtctcgac gccctcctct
     3601 cgatcgccca gatgccacgg ggggtccctg tggcctgcgt cggcgtggat aacggcgaga
     3661 acgccgccct gctcgcagtc cggatcctcg gcgcgcacag cgcctgacgg aacatttttc
     3721 gcccgtccgc aaggggaggg gcatgaagcg gagagtgctc ttcaccgctc catccgctct
     3781 tccggcggcg ccttgaggat atcccgcacc atctttacgg cgcagaggtc gccgcacatc
     3841 gagcaggtct cgatctcccc gtcccgctcg tggatgcgcc gcgcctcttc cgggacgagc
     3901 gccgcctcga actgtttatc ccagtcgagc gcccggcgcg cctccgccat ccggatctcg
     3961 cggttcttcg tcttcgccgc ggcgcgggag aggctcccga cgtgcgcagc gatcctcgcc
     4021 accctggtcc cctccacgat gtcgcggagg tccggcagcg ccaggtgttc gctcggcgag
     4081 accatgcaga ggaagtcggc gccgttcatg caggcgatca cgccgccgat cgcccccacc
     4141 acgtggtcgt agcccggcgc cacatcggtc acgagcgggc cgagcaggta gagcggggcg
     4201 ccgtcggtca gttccttgag catccggaca ttgtagccga tctggtcggc cgggatatgc
     4261 cccggcccct cgatcatccg ctgcaccccg gcggcgagcg ccttcttcgc caggtgcccg
     4321 agcgtcaggt actcggtcgt cttggcgagg cggccggcgt ccacgagcgc accnnnnnnn
     4381 nnagcaggta gtcgtactcg gcatagagcg ggttctcttc gccggtcgcg gccatcatcg
     4441 ccacgtggaa ggccccgccc cgggaaacga cgcccatcgt ccgtgggtcg gcctggagcg
     4501 acgcgagggc gtcgcggttc acgccgcagt gcagggtcag gaagtccacg ccctgccggc
     4561 agtgctcccg gatcaccttg aagagcaggt cggcgtcgac gtccgcggca gcccccgccc
     4621 gccggaccgc ctcgtagatg gggaccgtcc cgacgggcgc gtcgagagcg aggacccggc
     4681 gccggatgcg ggggaggtcg cctccggtcg agaggtccat cagcgcgtcc gccccctcgc
     4741 ggagggccgc tttcgccttc tcgacctcga ggtcctcgtc gcacctggtg cccgacgtgc
     4801 cgacgttcac gttgacccgg accctgcagc cctccccgat ggcgcagagc cggtgtggcc
     4861 gcacggggtt cgctggaatg acaatccgcc cacgggtgat ggcccgcgcg gcgtggtgcc
     4921 cgacgaggtc ctcctcccgg gcaagcgtct ccacctccgg ggggaccccg cgcaggcacg
     4981 cagagatcag ggtatgcata agaaccattt gcccggaagc catattagtc tgcatgccag
     5041 tccggtggat cgcgagcaat gcgacctacg ccaattcgtt cgtttacgga aacatccttg
     5101 tcgacgcggg cgttcccccg atggcggtgg agccgtatgc cggggcgatc gagacgatcg
     5161 tcatcaccca tgcccactac gatcacatcg ctcatgtccg ggagatcgcc cggatatgcg
     5221 gcgacccgac cgtctgcatt catgaggcgg acgcgcccgg cctcgtcgac gacacccgga
     5281 gcctcgccat gcactttggg gcccggtcgc ccgggatcgt ccccgacacc ctccttcacg
     5341 acggcgaccg gatcgggagc ctccgcgtga tccacacccc cgggcacacg ccgggcggga
     5401 tctgcctcta tgacgaagtg gggaaggtgc tcttctcggg ggatacggtc tttaccggcg
     5461 gatccttcgg gcggtacgac tttccgggcg gggaccggac cgcgctcgcg gcatccatag
     5521 agcgccttag cacgctcgaa gtcgttggcc tctaccccgg gcacggggag cccgtcgccc
     5581 acgacggcgg gaggcatatc gcagccgccc gggaggcgct ccggttttat ggataaggga
     5641 gtctacgccc tgatcctcga aaactccggt tgtgaggtcc gcatcggcgc cctcggggta
     5701 agggagttcc cccgcggccg gcacgtctac atcggttcgg cgcagggaag cggcggcctc
     5761 gcccgggcag accggcacgt gaggctcgcc ctccgccgcg accgccctcc ccggtggcac
     5821 atcgatcacc tcctcctcga cccgcacttc gcgcctgccg ccgtcgtcac cgccgctacg
     5881 gatcgggact gcgagtgcga cctcgcccgc gccgtcggcg gagcatacat ccccgggttc
     5941 gggtgcagcg actgcacctg cccctcgcac ctcttctacc gttccggcga cccggtcccg
     6001 gagatgctcg cctgctttcg ggatctcgac ctcgatgccc ggatcacaag aatcaagaac
     6061 gaggggagcg agcactaggt catgaatgtt cttggtatct ccggaagcat gcggaagaac
     6121 ggcaacaccg cgctgctggt caccaccatc ctcgaccggg tgcgggaggc gggcatcgag
     6181 accgaatacc tgacgctcgc cgatatggac atccggccct gcaccgggtg cgaagcctgc
     6241 cgggaggcaa agtggtgcgt gatggaggat gacgactggg ccggtgtggc acagaagatg
     6301 atcgactgcg aggtgctggt cctcggcgcc ccgacctact actacgacat caacggccag
     6361 acgaagaacc tgatcgaccg gacctactcc ctcttccacg acaggaggct ttcagggagg
     6421 agggccgtgg ccgttgccgt ctgcgctgac cgggggggcg agcgctccct cgagaccata
     6481 gaggggttct taaacgccca tcagttctcg tacctcggct acgtctgcgg cagggcctat
     6541 gcgccgggag acattgtgaa ggacgagcag gcgatgcaga gggccgcaac ggttgcggat
     6601 acgatcgtca gatacctccg gccggaggac tgagagcccc gtctcagccg gtcatgctcc
     6661 tcggggcggg cagggggctc ttccgaagaa aggggccccg gtgttgttgg agatcgaggg
     6721 ttccgggtgc cgccccgccc gtataccggg ctcatcggcc ggtgaacggc tcgtgagccc
     6781 ggctggcggt cagagcagca gcgatattat cgcgaggacg atgaagatga tgaccagcca
     6841 cttggcgatc gacatcgaca tccctgcaat gccccgtgcc ccgaatatgg caaagacaag
     6901 tgccagtacg aagaagagga ttgcaaggcc tacaagaccg tttaccatct ttttctcccc
     6961 tcaatcgccg gcatccggcc ggcgtcgagg agctataatc aatttttaga tatttatatt
     7021 tttcttggca gattggaggg gtggggcgaa gcgatccggg tccggccccg gatcagtccg
     7081 ggaggagggc ataggttgtt gtccgttgtc ggagcgtccg tccgaggtcc gtggccatcc
     7141 ggcgcatctc ggcggggtcc aggtaatcgg tatcccgggc acccgcctcc cgggagatgc
     7201 tctcctcaaa gagtgtcccg ccgaggtcgt ctgcacccga gagaagcgca agctccgcca
     7261 tcttcgtccc gagcttcacc caggaggtct ggatgtggtc aatgttatcg aggaagagcc
     7321 ttgatactgc aaacatcagg agatcctccc tccccgtcgc cccgggccgg gcgagccccg
     7381 cgcggtagag aggggtgttt ttgtggatga acgagagggg aacgaactcg gtgaaccccc
     7441 cggtatcgtc ctggatctcc cggaggaccg cgaagtgccg ggcccggtcc gcctcggtct
     7501 cgcagtggcc gtacatgatc gtcgccgtcg accggatgcc gagggtgtgc gcctctttga
     7561 tgacccgggc ccaggtcgcc gtgtcgatct tgtccgggca gatcaccgcg cggacgctgt
     7621 ccaccaggat ctcggccgcc gtcccgcaga gcgtcccgag tcccgcggcc tgcatctcca
     7681 gcagcacctc acgggtggag agaccgcttc tcttcgcggc gtaggccacc tccatcgggt
     7741 tgctcgcgtg gatatgaacc ccgggagcct cgtcccgtat ccaggaatag atatcgacgt
     7801 aggattgagc gtcaaactcc gggtggaggc cgctcaccgt gcagatttcg gtcacgttcc
     7861 gctcacgcgc catccgggcc ttctcccgga cggtggcttc atcgtggaaa aacacatcag
     7921 cgtcgcccgg tttccgggag aacccgcaga acccgcaggc gttcacgcag aggttggtca
     7981 cgttgatgtt ctggttgcgg acgtaggtga cgacatcgcc gaccttctgt tcgcggaggg
     8041 tatccgcggc ggcggcgatc tcccagacgc cccggtcgtg tgtccggagg agacggacgg
     8101 cctcctcctc ggtgagccgg tgaccggcga gcacgtcgtc aagcatctgt cgtagtggtg
     8161 gtgtcataga cctcaaccgt ctttcctcag gggataattt cacttggcgc ggtcaggaca
     8221 agaaccttcg ggtgcgcccc gggggagggc atacacttat cagttcccgg gggtatcccg
     8281 gcacctatgg ctcagcagat cctatgtcac cagcgcacaa ggctcagcta caaggacgca
     8341 tacggatacg tcatcccggt cgggagcacc agcctgatgg cctgggtgac cgacgagggg
     8401 atgatcggga ccgccgggtt cgatatcgag gctctggcaa acgccggggt tcctgcggcg
     8461 atcgtcgggg tctcggaaga gtccagcctg gagaacctcg acggcctgat ggacgccgag
     8521 gtgagggcgg taaaccttcc ggcggtgcag cgccgcgtcg aggtcgggat gccggggaga
     8581 gaggccttga acaggatgtg agcaggcttc gcaccttaaa ccggtgcgaa ccggccttcg
     8641 gccgggctca caccatctcg taggtcgtcg tccgctgccg gagggaccgc gcgaggtccg
     8701 tggccatccg ctgcatctcg cccgggtcca ggtagtcggc cccttcgccg ccggcgtcga
     8761 ccgagacgtc gtcgcagaac atcgtcccgc cgaggtcgtc cgcgccggaa aggagcgcaa
     8821 gctccgccat cttcgtcccg acctttcccc aggagacctg gatatggtcg aagttgtcaa
     8881 ggaagagccg ggcgaccgcg acgaggagga tatcttccct gccggtcgcc cccgccgggg
     8941 cgagccccgc ccggtagagc gcggtattct cgtggaggaa ggagagggga acgaactcgg
     9001 tgaacccccg ggtatcgtcc tggatatccc ggaggaccgc gagatgccgg acccggtccg
     9061 cctcggtctc gcaggagccg tacatgatcg tcgccgtcga gcggagcccg agaccgtgcg
     9121 cctcccggat gacccgggcc caggtcgccg tgtcgcattt cctcgggcag atcaccgcgc
     9181 ggatcctgtc caccaggatc tcggccgccg tcccctgcag ggttccgagc cccgcctggc
     9241 ggagccgctc gatggctgcg gtggtcgaga cgccgctttg tcccgccgcc cagacgatct
     9301 cgtccggact tgcggtgtgg atatccaccc cgggagcgac ctcccgcacg caggagatga
     9361 ggtcggcgta ggagtcgagc gtgtagtccg gatgcacccc cgagaggagg cagatctcgg
     9421 tgacgttccg cgccaccgcc gtccgggcct tctcccgaac ggtcgcgagg tcgtcacaga
     9481 acgcctccgg atcatccgcc cgccgcccga atccgcagaa cccgcagagg ttcttgcaga
     9541 tgttggtgac gtggatgttc tggttcctga cgtaggtgac gacgtcgccg actttgcgtt
     9601 cgcggagttc atccgcggcg gcggcgatct cccagacgcc ccggtcgtgt gtccggagga
     9661 gacggacagc ctcctcctcc gtgagccggt ggccggcaag cgtatcatcg agcaggtcat
     9721 gcagcgagtg cggtatcgta aacaactccc ttgtaatcct ggatttattc ttctttgcgc
     9781 cgcgcgagga cgaagtcgtg gatgaggatg acgacgatga cgatgacggc aaactccgca
     9841 aggtgcaggg gcaggtaacc gatgagcggc accgcgacga gggctttgcc cacaatccag
     9901 tccttcttga ccggctcgac caccccgaca ccggcaatcc ggaggttccc ctggtcgatg
     9961 taggggttgt tgtccccttt ggtgatgtag ccgccatggg gatcctcgta gtattgcgcg
    10021 agccccgact cctccacggc ggcggtatcg acgtaagtca tcgcccggtg aatgatcggg
    10081 tggacggagt cagcgccgtt cgggcgatag acgatcacgt tgccgtagtc cccgaaggac
    10141 gaatccccgg tccgttgccc ctcggaccag gtcacgagac tgccgaaccg gtcctcatcg
    10201 acgacgagca ccaggtcgcc gacgttcatg ttcgggacca tgctctccga ctcgatcgtg
    10261 accaccgccg gccaggtgcc ggcgaagagg tagaggacga gggcgatacc ccccacgacc
    10321 gcgacgaccc agaggaggtc ccgggcgaga gagacgaccg ggtgctcgct ctcccggaac
    10381 gcctgcaggg ccgatgctgc acgctgcagc caggtggtat ccgacattgc tctaaagaga
    10441 atgctatccc ttcatataca taggttctcc gggatacccg gggagatggg atactaaaaa
    10501 gataaaccca tattgcctgg gggagaataa gatggagtcc atgctcgcca acgccgagat
    10561 cgtacgccgg ttccttgagt tgaaacatca ggtccatccc gacgtggtga gttacatccg
    10621 tgagcagaac gacccggcgc tcatcgaccg gatagcggcg agggtccccg gcggcaccct
    10681 cgtcatctcg gccgaacata tcccgggcct ccgaaaggtc cgggacggga cccgttttct
    10741 cgtcgacccg gaactcgagg tgatcatggg gagcgccggc accacgggga gcgtcaccgg
    10801 tcaccaggac gccgtccact acttccggga ccgctacaac agcctttcat cgatgatccg
    10861 gagccggatg aacaccatgc cgatcgaggc gctggtcaaa tccccccacc gctaccggga
    10921 cgaggagtgc agcatcatcg ggatggtggc cgacgtgcgg acgaccgcca gggggcaccg
    10981 gctcgtcgag gtggaagacc ccaccgggac catccgggtg ctcttcaata aaggaaaaga
    11041 cgacgccttc ggagaggcgg agcgtatcct ccccgacgag gtgatcgggg tcaaagggaa
    11101 actctcaaac gacggagggc tcttcttcgc cgagcagctc tcccgtccgg atgtccccat
    11161 caacaacgcc ccgttcaaga gcgatcggcc ggggaaggcg gtcctgatct ccgatgtcca
    11221 tgtcgggagc gacaccttcc tcgaagggga gtggaaccgg ttcgccgact ggctccagga
    11281 ctccgacgcc ggctacctcc tgatcgcggg cgacctcgtc gacggcatcg gcatctaccc
    11341 cgggcaggag aacgaactga cgatcaagaa catctacgag cagtacgatg tctttgcaga
    11401 gatgctccgg gacctgccct cccgggtccg gatcgttgcc gccccgggaa atcacgacgt
    11461 cgtcaggatg gccgagccgc agcctgcgat cccgccggag ttcaccggga ggttcccggc
    11521 aaactgcacc ctcgtcgaga atccctgcct gctgaacctc cagggcgtcc gggtcctcat
    11581 gtatcacggc cggtcgatcg acgacatgat cgggcttatc ccgggagcga gttacgagcg
    11641 gcccggcgag atcatggagg agatgctgaa acgccggctg ctcgcctccc cctacgggat
    11701 gcggaccccc atagcggcga tgaagaccga ccggctcgtc atcgacccgc tccccgagat
    11761 cctcctcacg gggcacgtcc acatctgcgg catcacccgc taccggggtg tcctcggggt
    11821 gaacaccggc acctggcagt cccagactgc gtttcagaaa cagatgaacg tccaccccac
    11881 acccgcgcgg gcatttgtca tcgatctcca gacactccag ccggaagtga tggatttcag
    11941 ttaaacttcc atcaggtccc ccggatatat tccacatatt taaaagtgtt gaatgccttt
    12001 gtatagggta ctcaatcgga ggaatgcaaa gagatggatc tggtgtatct ggcacccatt
    12061 ggtgccattc ttgctctcct gttcgcagga tactcgtata tgagtatcag gcgggagggg
    12121 acaggtaccg aacagatgca gaagatcgct gccgccatcc atgatggggc gatggtctac
    12181 ctgaacagac agtaccgtgc tatagcaata ttcgtcgtcg tgcttgccat cgtcctcggc
    12241 gtctggatca gcccgttcac cgcagcctgc ttcgtgctcg gtgcagcgct ctcggcgacc
    12301 gccggctaca tcggcatgtt caccgccacg gccgcaaacg tccggactac gaacgccgca
    12361 cggagaggga tcgcagatgc gttccgggtc tcgtttgcga gcggttcggt catgggcatg
    12421 gccgtggtcg ggctcgggct cctcgggctc tcgatcgcgt atatggtcat cagcaccgta
    12481 acagggcttc cccaggctga agtcctcacc atcgtgaccg ggttcggcct cggagcgagt
    12541 tcgatcgccc tcttcgcccg tgtcggcggc ggtatcttca ccaaggcggc agacgtcggt
    12601 gccgacctcg tcggcaaggt cgaggcaggc atccccgaag acgacccccg gaacccggcc
    12661 gtcatcgccg acaacgtcgg cgacaacgtc ggcgacgtcg ccggcatggg tgcggacctc
    12721 tacgagtcct acgtcggctc gatcatcgca gcgatggtcc tcggcgtcgc cgtcgcggcc
    12781 acgcagttcc cgaacgccga cgtcatgaac gtgatcatcc tcccgctgct gatcgccgcg
    12841 gtcggtatcg tcgcgtccat catcggctcg ttcttcgtcc ggaccaacaa gaccgagagc
    12901 agcgccatcc acatggcctt caacaaaggt ctgctctccg cgctcgtcct ggtcgtcatc
    12961 gccacctact tcctggtgaa cctgctcctc ggggcggagt acatcgaggt cctctacgct
    13021 gcggtcgccg gtctcatcgc agggttcgct atcggcctga tcaccgagta ttacacctcg
    13081 tttgaccgga aaccgacgct tgccatcgtc aagtcgagcg agaccggggc tgcgaccacc
    13141 atcatcaccg ggcttgccaa ggggatggag agcaccgtct ggccggtcct gatcatctcc
    13201 ctcgcgatct acgtctcgta ccaggtcgcc gggctctacg gtattgccat cgccgccgtc
    13261 ggcatgctct cgacgctcgg gatctccctc tccgtcgacg cctacggccc ggtcgccgac
    13321 aacgccggcg gtatcgccga gatgtcccac cagaagcccg aagtccgtga gatcaccgac
    13381 accttagacg ccgtcggcaa caccaccgcc gccatcggca aggggttcgc catcggctct
    13441 gcggcgctga cggcactcgc cctcttcgct gcctacactc aggcggtcgg cctctcggtc
    13501 atcgacatat cggttccgaa cgtcttcatc ggtctcctga tcggtgcaat gcttcccttc
    13561 ctcttctgct ccatcacgat gatggcagtg gggaaagctg catccagcat cgtccacgag
    13621 gttcgccgcc agttcaagga gatcaagggc ctcatggagg ggacggccga acccgactac
    13681 acctcctgca tcaccatctc cacgaactcg gcgctgcgcg agatgatcct gcccggcgtc
    13741 ctcgccgtcg tcgccccgct cgccgtcggg ttcgtcctcg gcaccgaagc cctcggcggc
    13801 ctgctcattg gctcgctctc atccgggttc ctcctcgccg tcacgatggc gaacgccggt
    13861 ggggcctggg acaacgcaaa gaagtacatc gagcagggcc acctcggcgg caagggttcc
    13921 ccggcccaca aggcggcagt tgtcggcgac accgtcggcg accccttcaa ggacacctcg
    13981 ggccctgcca tcaacattct cttaaaactg atgtccatgg tcgcgctggt cttcgcgccg
    14041 ctgatcctgg tcatctaatc cccttttttc gagcccgata tcctgacggg cgtccatacc
    14101 ttcatcccgc ctgcagacaa ccagatagag tgtgaactcc cggagtggag gtgacagagc
    14161 gcatgttccg catttatcgc acggttgaag cggccccggt gccacggacg gaggagaccg
    14221 gaacgttcga gcccggttca tgggtctgca tgatcaaccc caccgcgggc gagatcgagg
    14281 agatctccgg gagactctcc attccagcgg actacctccg ggcagcgctc gatgaggagg
    14341 agcgcccgcg gaccgagacc gaagacggcg taaccctgat cgtcatcgat atcccggtgc
    14401 cctaccccga gcagactatc ctctacacga cgatgccgct cgggatcatc gttacgtccg
    14461 ataccatcgt caccgtctgc cttcgtgaca acccgatcgt ccaggacttc acgaacgccc
    14521 gggtgaagtt gttctacacc ttcaaaaaga cccggttcgt actgcagatc ctctttcgga
    14581 acgcatccta cttcctccag tacctgcgcc agatcgagcg attatccgac aggatcggag
    14641 ccgaactgaa tcagtcgatg cggaaccgcg aactgatcca gttgatgaac ctcaaaaaga
    14701 gcctggttta cttctcgaca tcgcttcgga gcaaccagat tgtccttgac aagactctga
    14761 cgttccagcc gctccggatg tacgaagacg acaccgacct ccttgaggac gtcatcatcg
    14821 agaacaagca ggcgatcgag atggcgagca cctactcgac gatcctctcg gagacgatgg
    14881 acgccttcgc ctcgatcata tcgaacaact tcaacaacat cttaaaactc ctcacctcga
    14941 taaccatcat cctcgcggtc cccacgatga ttgcgagttt cctcgggatg aacgtcccgg
    15001 tccccctcca ggacaacccg tacgggttcc tcatcatcat catcctatcc ctgatcgtct
    15061 cctcgctcct cgcggtggtc atcaaccgga aggggtggct gtgagacctt tagaggggaa
    15121 ccgggtccgc tggccgggat ccggcaacca ctgttctctt cgcacctgac ggtgcccgaa
    15181 ctcctccccc tttgggggag gcccacttcg aagcctgagg gcttctcatg ctcgcttacg
    15241 ctcgcacttc gcgtgaggca ggttatcatc cttcatgcag cgtgccgcgc ggacgagagt
    15301 ggtcgacccg gggtacggtg caccccggag ggctccggct ggcgtcggaa atctggtgca
    15361 gtcgccctgg gcaccacaag gtatatcacc gatcctacat gatagatcat gtataatctg
    15421 ggggcgaccg cacgggagga ggcgcccgga gaaattacgc tcccggcggg gaccggcacc
    15481 ggctgccggc gaccgcatgt acggcagcgc gaccacgggc cgaccggtcg gaaagacgac
    15541 ggacccggag atcagccgct tttgcacggt gtcggaaccc ggacgggtaa cggccggagc
    15601 aatccggcat gattaatcat gtaagagtgt gggaaggtga cagatacgtt cgacgtgaaa
    15661 ctggaagaga aacgctacct ccctgacccc cggtgcaagg cggcagcctg gacgccggat
    15721 tacacccggg cgtaccagga cttcgcgcgc gatccggagg ccttctggga ccggatcgcc
    15781 cgtgaactgg tctggttcaa gccctgggac agggtcaggg aatggaacta cccctacgca
    15841 aagtggtttg tcaacgcgaa attgaacatc acctgcaact gcctggaccg ccatgtcacc
    15901 ggcgaccgga aggataagcc ggcgatcgtc tggcgcggcg aagggggaga ggagcggaga
    15961 ttcacctatg acggcctcta ccgtgaggtg atgcggtttg caaacggcct cgcggacctc
    16021 ggcgtcggga agggcgaccg ggtctgcatc tacatgccgc tcgtccccga gcaggtcgtc
    16081 gcgatgctcg cctgcgcccg tatcggggcc gtccactcgg tcgtcttcgg ggggttcggg
    16141 cctgatgcgc ttgcgatgcg gatcaacgac gcggaggcaa aggtcctcat caccgcagac
    16201 gttgcctacc ggcggggaaa gaccgtcccg ctccgggaga tcgccggcca ggcgctcacc
    16261 cggacgccgt gcgtcgagcg ggtcgtcgtc ctccggcgcg aaaccccggc cgtcgacctc
    16321 gaccccgccc gggaggtcga ctacgccgac ctcatggccc acgccgcgcc ggactgcccg
    16381 gcagaggtga tggactcgga ggacccgctc ttcatcctct acacgagcgg ctcgaccggc
    16441 gcgccgaagg gcatcgtgca tacctgcggc ggctacatgg tcgggaccta ctacaccacc
    16501 cgccacgtct tcgacatcaa ggagaacgat gtctactggt gcaccgccga tcctggctgg
    16561 atcacgggcc acagctacat cgtctacggg ccgctggcca acggcgccgt cagcctgatg
    16621 ttcgaggggg tgcccaacta ccccacctcg tcgcgcttct gggaggtgat cgacaagcac
    16681 aaggtcaaca tcttctacac cgcgcccacn nacgggccac agctacatcg tctacggccc
    16741 gctcgccgtc ggcacgaccg tcgtcatcgc cgaagggacg cccgactacc cggacccggg
    16801 tgcctattgg cggctggtcc aggacctcgg ggtgacgatc ttctataccg ccccgaccgc
    16861 catccggatg ttcatgcgct acggggacga gtggccggca aagtacgacc tttcaacgct
    16921 gcgggtgctc ggctcggtcg gcgagccgct caaccccgag gcgttcgagt ggtactacca
    16981 caccatcggc ggcgggcggt gcccgatcgt ggacacctgg tggcagaccg agaccgggat
    17041 gcagatgctg accacgatgg tcggggagcc gatgaagccg gggtttgccg gaaagcccat
    17101 ccccggggcc gtcgtcgacg tcgtcgacag gacgggcaag gcggtcccgc cggggaccgg
    17161 ggggttcctg gtcatcaggg agccctggcc cgcgatgctc cggacggtcc acggcaacga
    17221 cgagcgctac cgcgcctact ggaacaccat cccgggatgc tatacagcag gcgatctcgc
    17281 ggtgaaagac gaggacggct acatcatggt catcgggcgg gccgacgacc tgatcgtcgt
    17341 ctccgggcac aacatcggca ccgcagaggt cgagagcgcg ctcgtctccc acgaggcagt
    17401 ggcggaggcg gccgtcatcg ggaagcccga tccgctgaag ggcaacgtca tcaaggcctt
    17461 cgtcaccctc cgcgtgggcg cgagctccgg cgacggcctc tccgccgaac tcgcccgcca
    17521 cgtcaaaaag agcctcgggc ccatcgcggt gccggccgag atcgagtata tggaccggct
    17581 tccaaagacc cggagcggca agatcatgcg ccgggtctta aaggcccggg agctcggcat
    17641 ggacccgggg gacatctcca ccctggagga gtaaccccat gcccgatacc ccgaagatct
    17701 tcggcatgcc tgcagagaag gggcggtggg gcctcgtggc cctcggtctc gtcatcaacc
    17761 tctgcctcgg gagcatctac gcctggagtg tctttgtcgc gccgcttgcc gcgcacttca
    17821 ccgcgacgct cggccgggaa gtgacggcgg gggaggtcct cctcccgttc tcggtcttcc
    17881 tcgccttctt cgcgatagcg atgccgctcg ccgggaagta tatcgaacgc tacggcccgc
    17941 gaaaggtgac gatcgtcggc gggcttctca ccggcctcgg gtggctcctc gcctcgacgg
    18001 cgacgtcggt cgaggcgctc tacgttgtct acggggttat cggggggctc ggggtcggga
    18061 tcgcctacgg ggcgccggtc gctgtcgcga cccggtggtt cccggaccgg cgagggctcg
    18121 ccgtggggct tgccctgctc gggttcgggt tctcggcatt catcaccgca aacgctgcgg
    18181 gagccctgat cgccacgtac ggggtgatgg cgacgttccg gatcttcggg gctgcgctca
    18241 tcgccgtgct ggtcctctca gccctgccgc tccggttccc gccggcagga tggcagccgg
    18301 cagggtggac gcctcccgca ccgggggtcg gcacggcgcc ggtctgcgag tgcgaccgca
    18361 gcgcgatgct ccggaccggg acattctacg ggctcttcgc ctgctacttc gtcgggtgcc
    18421 tcgcggggct tgccgcgatc tcgatcgcaa aaccggtcgg gaccgaggtg gcgggggtcg
    18481 atgcggggct cgcgacggtc ctcgtcgggt tcttcgccgt cttcaacggg ctggggaggc
    18541 cggtgttcgg cggtctcgcc gaccggatat ccccccgcac cacggcgatg ctgaccttcg
    18601 gtctcatcgc ggcggcatcg ctgctgatgt ggctcgtgcc gggggtgccg gcctacatcg
    18661 tcgcgttcgc cgtcctctgg ggatccctcg ggggctggct cgccatcgcg ccgacggcga
    18721 cggcgtccta cttcgggacg tgcgactacc cgcggtgcta cggggtcgtc ttcctcgcct
    18781 acggcgcagg cgccatcgcc gggccgcagc ttgcgggatt cgtccggacg gcgaccggga
    18841 cctacctcgg cgtctttccg ctggtcgcgg ccctcgccgc cgtggggttc gtcatcgcct
    18901 ggctcctgat gcggccgccg gccggcgccc cggcgagtgc gcccctgccg gcggcggtgg
    18961 aagagtgagg agaaaaggtt acactttccc ggtgagcccg agccgccgga gcatcccggc
    19021 ggctgcgttc attccgccgt ccaggtagac ggcgccggcg agcgcctcca ggcactcggc
    19081 gaggacccgg ccggaggtcc agaccgcctg ggcggcctcc ccttttcccc agcggacgta
    19141 gtcggcgagg tcgaggtctt ccgcgagccc cctgagccgg gtcatgttca ccaggttcat
    19201 ttttctgttg gtgatcgcgc ccttgtcgtg cgccccgccc gcgaccaccg cctccacgac
    19261 gatcacgttg atgaccgcat cgccgagggt ggcaagagcg tccatgtggg tctcgggggg
    19321 gagacccgcc tccagggtgt acgcgaggcg ggtgagggcc cgctcgagga gtgcgcggtc
    19381 attgaaggtg tagccgatcc gctcctcgat cacgcccggg ttccggcggc cggcgccggc
    19441 gtcgctgcgg ctcataggtg gaggggcgtt actccacgac ttcggccttc tcgatccgga
    19501 cggggatctt cggcttatcg gaagagctcg tcttcacctt cgcgatcttg tcgatgacgt
    19561 ccatcccctc aaccacctct ccaaagaccg ggtgggcgct cggcgtctcg gggttgtccc
    19621 agtcgaggaa gtcgttgttc accagattga tgaagaactg gctgccgccg ctgttcgggc
    19681 ggccggtgtt cgccatggcg atcgtgccgc gggcgttcga atggcccttc acgaactcgt
    19741 cggtgatggt gtagcccggg ccgcccgtcc cggtgccggt cgggtcgccg ccctggatca
    19801 tgaacccggc gatcacccgg tggaagatcg tgccgtcata gaacccggat ctcgccagtt
    19861 ttgcaaagtt ccccgcagtc acgggcatgt cgtcgtaaag gcgaatcgta atatctccca
    19921 tggtcgtgtg gagcacgacc tgcgttccat tcttctggtc agtcataaga aaatctccgt
    19981 aaggtattgt ccgccgttgc tacttatatg atatgcgggg gtacgccccc tcacgcgccg
    20041 gtctcctcgc cggggtagaa cgggcattca taccccgtgc agtcgcggtt tatggtggcg
    20101 tactcgcacg cgatatgctc cgtcaccggc aggctgatgc cgcacttctc ggcgaggaac
    20161 ttcaccgcct cgccaatcgc gagccacgag tcccgcttgc agcagcgcgg cccgccgctc
    20221 acggcgatca ccgcaagact ccgcgccgtc atctggttcg agagcgacca ctcctccttc
    20281 ttcagcgggc cggagtgcgt gatcaggctc atgaagatcc cggtgccgat tgccgcgccg
    20341 caggccccgt gggtgccgca gaaggccggc tggacggcct ctgcccgctt cctcgcctgc
    20401 cggagcgccg aatccttctt ctccgggctc ccccggtggt tgtagtaggc cgcgatcagg
    20461 acggccggca ccaggaagtg gtgttccggg ccgtggaggc tgatctgggg ccgctgcatg
    20521 atcctgcagg cgattgcgag cgggtcctcg agcgtcgtcg tcctgcagac ctgctcgatc
    20581 aggtcgaggt tcgagagctc gcggtcggcc ccggagaggg cgtcctcccc cggcggtgcc
    20641 gtctccggtc ctttttcgcg cgggacgagg ttctttctgc aggccgtgca ggccttctcc
    20701 tggtccatgg gcactcctgg cgccgggcct cgcataaggg ttacgggccc tgctcccacc
    20761 cgcaagtaaa ccacacggat ttaacaataa caataagtaa aagtgtttta taccgaaagc
    20821 cgcaatgtat acccatgtac tccggagcga acgtcaaccc ggaccccggc cgggatatgc
    20881 ccgggtcccc caattcagcc ctcataaccg gaggtctccc cggatgagcg accggacgcc
    20941 ggtcttcatc atcaccggcg ggcagggcca gtgcaagacg acgttcctgc acctggttct
    21001 cgggttcacc gtcgggctga acgtacgggc ccggggcgtg atcgctcccg gtcatatgcg
    21061 ggacgggcgg aggtccgggt tcaccctcgt cgacctcgcc accggcagac atgaagaact
    21121 ctgttcgatc gaccccgacc cccggtgcga ggtccacggc cgattctact tccggcccga
    21181 agggcttgcg ttcggccgcc gggcgctcgc accgccggac ccccgggaga ccgacctggt
    21241 ggtcatcgac gaggtgggga ggttcgagct ccagggtgcg gtctgggcgg agcagctgga
    21301 tcaactgctc gcgcacccgc acccccccat ggtctggacg gtccggcgcc ggctcctcga
    21361 tacggtcatc ggacgctgga acattacaaa tcccgtcgtg gtggacgtcg ggacggcgag
    21421 cgtgatggcg acggcaggag acgtgatgga ggccgtctgg gagtggcggg aggcgcagac
    21481 ggcctccggg actccggctt tcccgctccc ccggggctgt accgccggcg atcccccgct
    21541 gcttgcggat gcagccgccg gaggaacgcc aaacgctccg gcgatccttc ggcggcgcta
    21601 cggcccggag gagtgagggg gacccgcacc cacgtctggt tcaaaaaagc agaacaacaa
    21661 cagcaacagg cgctgccgaa acagcaccgc cacttccttt tttgctctct tgactcgtga
    21721 actacccccc gcttgcgcag ggggcttcct gcgagtgtcc ggggctctcg gggagcctca
    21781 gacctgtttg tcgcagattg ctggcgaact gccggtagcc cgtccac
//
LOCUS       DAJB01000018           16767 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_2753, whole genome shotgun sequence.
ACCESSION   DAJB01000018 DAJB01000000
VERSION     DAJB01000018.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16767)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16767)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16767
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_2753"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3363 a         5314 c         5088 g         3000 t
ORIGIN      
        1 gtgcgggatg cggccaacgt gcggtatccc gtaccggtga acgtcaactc cgcatacgcc
       61 ctgatcaaga aacccgcgct ccgggtggag aggagcgtcc ccgcatccgt cgacccgggt
      121 gaccccttca acgtcacgct cacgctctac aacgagggcg gggcaagcgc aagcgacata
      181 tccgtcagca tcaacgcaac ctccggcgcg atcaccgcag gcaactcgga gaactacttc
      241 atccccgaac tcggggcagg ggaggagtcg gtcctgaacc tctccttcga gaccgatacc
      301 agcgcacccc tcggactcac accgatcctc gtcaccatcg actaccggag cgccgatctc
      361 gctacgttcc ggcaggtggc gacgatcggt gttcccatcg tcggccgcgc cgagatgggc
      421 atcgcctcca tcaggaccga accctcccgg atcaccgccg gagacccggt ggacctgacg
      481 atccggctgg agaacaccgg gacggcggac gcggaatcgg tccgggcaac cgtcgagggg
      541 ctctctcttc ccggtacaaa agaggcgttc ctcgggacaa ttgagcccgg caacgacggt
      601 cccgccgtct tctccctgca ggccagcgaa gcgggagagt tcgactacac cctcaccata
      661 cagtatgccg acgactacgg ggcgcacaca acccggcagg ccctgcaggt tgttgttgtg
      721 gaatcaagcc cgatcccggc tatcgccgcc gcggcagcga tcctcatcgt cgtcatcgcg
      781 gccgcggcct tctggtaccg gagacgtcag gaggagtgat tccggtgttt gcaggcctca
      841 gggtctcggc gttcctcgcg tggaaatcca tccagcgcgg gagcagaggg acgctcgccc
      901 tcacgatcat gatcatcgcc ctcatcttcg tgaacctggt cttcctcccc tccatcatct
      961 ccggggtcgt cgagaccttc aacacccagt cgatcgactt caacttcggg aacctggtca
     1021 tcgagccccg ggagggcacc cagtacatca gcgatgcgac cggcctccag aggagagtcg
     1081 agcggatccc cgggatcacc ggcacatcat cccggatagc cgccggggcg accttcttct
     1141 accggggtcg gaacgccgca agcacgcttt acggcatagt accggaggat gagcggtcgg
     1201 tgacgaggat tgtcgagtac atgaccgagg gggagtttct ctcgaacgga gacaccgacg
     1261 caatcgtcat gggcacgacc ctcgcgggga cggaggggga ggaggcaggc ggccttccct
     1321 cgctccaggg gatcggggtc ggcgactcgg tggaggtggc ctacccgaac ggcgagatac
     1381 ggacctaccg ggtgaagggg atctacgaga cccagtcctt cagcgtcgat acggcagcct
     1441 tcatcaccag ccgcgagatg agcggggtgc tcggcctcca ggaccaggca tccatcatcc
     1501 tggtgaagac cgaggttaac ggcagggagg agtacaagac ggagatcctc tcctacggca
     1561 tccaggagga ggtccggacg ttccaggaga agtcgggagg gttcgtcgac caggctatcc
     1621 agagtttcaa catcatcaac gccatctcga cgctcgtcag cctgatcatc gccatcgtgg
     1681 tggtcttcat cgtgatcttc atcaataccg tgaacaggcg gcggcagatc gggatcttga
     1741 aggcgatcgg gatcgaccag cagatcatca tcaactccta cgtcctccag gtgctcttca
     1801 tcaccaccgt cggggcactg gccgggatag tgctgaacgc cggcgtgacg acctacctga
     1861 ccgcctaccc gctcgccttc cccggcggcc cggtctaccc ggccgtggaa gcgtcggcca
     1921 tcctccggag catcgcaagc ctctttgcgg tctcggtggt ggcgggctac atcccggcgt
     1981 ggcggatagc acgggaggat atcctctccg caatcagggg gtgaagggat gatcgtcgca
     2041 gaggacctca ggagggtcta caccatgggg agggtggagg tccgcgccct cgggggcgtc
     2101 tcgcttgagg tcgcaaaggg agagttcgtc gggataatgg gcgcgagcgg cagcggcaag
     2161 tcgacgctcc tccatatcct cgggctcctc gaccgccccg actccggcag ggtcacgatc
     2221 gacgggaccg atgtcctcgc cctctccgac caccggcgga ccctcttccg gctgaaccgg
     2281 ctcggctacg tcttccagga ctacgcgctc atcggggagc tcacggcact cgagaacgtc
     2341 tacttaacct cgcttgtccg gggggcatca aagaaggagt atctcgaccg gagcaccgat
     2401 atcctggagc gggtcggcct cggcgaccgg atggaccacc ggcagagcga actctcgggc
     2461 ggggagcagc agcgcgtggc gatagcccgg gcgctcgtga acaacccgag catcctgctc
     2521 gccgacgagc cctgcgccaa cttagacagc cagacctcga ggagcatcct cgacctcttc
     2581 gcccggttga acgaagacct ggaccagacg atcgtgatgg tctcgcacga ggagtggcat
     2641 aaggagtact tctgccgggt cgtcaccctg caggacgggt tgatccggga tataacggag
     2701 tgcaggaagg ggccgcgccc gggcggcggg gagtagcctc tcccggccgg tcgcttgtcg
     2761 cgaagtgccg caaggtggca gtgcagccgg gtgcagcaat tctgacctca cacgaggggt
     2821 tgcggcagaa aaaacgacga cgatattttt atatcacacg tgagatacaa ccccggccgg
     2881 tgaaagatat gcaggaagtc aggacattca ggtgcaagga ccttggcctt gcgtgcgagt
     2941 tcgaggagaa ggcagagagc gagaacgagc tgatcaagcg ggtcgagggg catatccaga
     3001 ccgcccacca gatgaacccg gaacagccgg aagtgcagga gaagatccgc aaggcgatga
     3061 agtgaggggc ggcaggttct tttgcgatgc acgccaccga gaccgatatg gtgcagcaca
     3121 ggcattcctg gagacactcg cccgaagatg aggccgcccg ccggctctgg cagcaccccg
     3181 ggtccatcct cgaggggatc gggcttgccc ccggggccac cttcatcgat gtcgggtgcg
     3241 gagacgggtt cttcgcactc cctgcggcgc ggatcgtcgg gccgcacggt cgtgtctgcg
     3301 ggatcgatct cgacgccgaa gcgctggaca tgttgcagga gaaggctttt gccggggggc
     3361 tcgacaatgt tgttctccac caggggatgg ccgaggagag cgtcctctgc gacggatgtg
     3421 cagatgtcgt cttcttcggc atcgacctcc acgacttcgt cgacccggtc cgggtccttg
     3481 cgaacgcgaa aaggatggcg ctcgacgccg gggtgatcgt cgacgtggac tggaagaagg
     3541 aatcgcttcc tctcggcccg cctcctgcga agaagttcag cacggagcat gcagcgtcgc
     3601 tgatggcgga ggcggggctc ctggttacga cggtggcgga gtcagggcca tatcattata
     3661 tcatcacggc cgtgccgggg cgctagccct tgaccggtga aaaaagggtt ttgaggttac
     3721 ttaaggttcg caagaccgcc gtagttgata gcatttacga acgcgtcgag atcctgcttg
     3781 gagccgtctt cgacgctgat atagaccatg aaccgttcat gtacgccgat ccaggccccg
     3841 taggacgcag gtttagcgaa gtactcccag gggggccgct gaccgtgatc ttgaagcgct
     3901 caccaacctc attcgcgacg gctgatcgga aaacggccga aacagggcgg atcggggggc
     3961 accattatat agagtttaag aaaagtattt aacaattcta agcgacctta tcgcatggtt
     4021 cagaaaaaga gtatctatgg agtcctgatc gcaggacttc tggcactgtg cctgctcggt
     4081 gccggttgca cgtcgcagcc ggcggaacag caaaacgtga ccgcgacccc caccccggcg
     4141 gaggagtacg tcttcaacga gacgaacaac aacgagacgg tcaccctccc ggtcgggagc
     4201 gagatcacca tcagcctcga tgagaacccg acgaccgggt actcctggaa cgtcacctct
     4261 tccgccgggc tctcgtacgt gaacgacacc tacatcgcac cggagaccgg gctcatgggc
     4321 gccgggggcg tgcatgtatg ggagtacctc gccgccgaga cgggcgccgc cgaattctct
     4381 gcgatctaca tgcagccctg gttaaacacg acaggaaacg agaccacatt ctcgatggca
     4441 ttcaccatcg agtaatcttt catcttttta tacgtatccg cggcgttcac cgcccgtttc
     4501 ctaaccgccg cagaacctct gcccccggcc aaggtcctcc gtgcgccgcg tagacccgtt
     4561 ccggcccaaa cgcgaggagc gtccggaggc tcttatgtgc cgccacgggg ttatccatcc
     4621 tgaagggtag ccggggcctc cccccggcaa agagagcaga aagaaggtcg ccgatgataa
     4681 cggtgccgcc ggcgacaagg accgagaccg agccgggagt atgccccggc gtggggagga
     4741 ccctgccccg gaccccgaag gccgtaaggt cggcgggact cctgatgacg atgtcgggct
     4801 caacgccgcc aaaacccggg acagctatcc taccgcctgc aagcccgatc aggcgcggga
     4861 tgatcctggc ggggaggcgc agcccgtcaa acccaggacg catagcgtcg gcatcggcct
     4921 cgtggacggc gaccggtgcg cctgtcagtt cccggagtgc gtcggcgctc ccgtagttgt
     4981 tcgggtgact gtgagtgacc aggatcaggg agacctcgtc gggactgatg ccggccttct
     5041 tcaccgcggc caggatggcc gtctcatccc cggggttgcc ggtgtcgacc agaagcacgc
     5101 tgtggtccct gacgatgaac gaggtggtca ggccgagcgg cacggggatg accgccggca
     5161 ggtagcgaac agcggtgcgc tccatctttt gtgccggagc ccggggagcc tctctactcc
     5221 cggcaaaatc ccccggatcc gattcgatgg tcttcacggc atctccactg tgagataccc
     5281 tctctccaca tcaatgattc ggtcaggaga agagaggggg accgggggtg agtttgcggt
     5341 ggtccgggag gttccccgtg agggacgggg cctccggcgg tatttcaata cgcatatata
     5401 tcgcggaaac gaggtgaaac tatgatctat aagagaatga catcccgata tctcctcgtt
     5461 tccctggcgc tccttgccct gatcctcgtc gccccggcag ccgcacaggg gaccagcccg
     5521 caggaggtgc aacccggcga cacgatcgag gtggggagcg aaccgctcgt cctcgacctc
     5581 gtcgacctcc gcaacgcaga tacattcaat ccgatcacgg aactccgaca ctaccgggac
     5641 gacgatccca caaagcagat catccgggtc attggcgtac cgaacgatga tcgcttcacg
     5701 atcaatacgc atacgctcaa cgggaagtac ggacggtact tcgcatacag cagcaaggac
     5761 ggcctgatcc agcagcacag catcgtattc acccccgctc ccggagtgac ggccacagag
     5821 tcgaccggga cccccgccac ggccacacag acccccacgg agacgggaac accgccggcc
     5881 acgaccacta cagccccgac gcaggcaccg ctcccgggac tgatcgcgat cgccgcgctc
     5941 gggatctgtg gactgttcct ggggacgaga cggcggtagg cagatcccgg agccccggca
     6001 gccaacggcc gggaacggtt cgcctgcagg tctcatctat cggctgagac aggccccggg
     6061 gacaggagtg cctccggatg cctttggcct gcaagcccgc cgcatcgggg aaccacaaaa
     6121 cgttttttgg gacctgttct ggggcttaaa ggaagccgtc tcacggagca tcgtgacggc
     6181 cgaactgacg gagacgtcct ttgaacccgc cacagccgcg gtctttcgga cacctgctcc
     6241 cgacgggtat tatatactgc tatggatatc ccccgccgga gaaggatcac ccgtttgtta
     6301 cccgcacccg ctggactgcg gcagaactcc cgggaacctt cctccttcaa cacgggggca
     6361 tcttcggccc ggcgctctgg agaggggggc tgcccggaga cccccaggag gcccaaacga
     6421 tgaacgaaca caatttctct gcaccggtgc tggtcctgat cgcccttgca ctcctcatcg
     6481 gtgccgtatc ggccgaggag ttccaggtca ccaacgcttc ggtggaccag gtgtattcaa
     6541 cggtgcttga cgaccgcatc ctctggtccg ataaccggac gggagactac gacgtctacc
     6601 tctacaacct gaccgaggag agcgaatgcg cactctcccc gccggagacc gaccagttcg
     6661 cgacggcact ttccgccggc atgatgatga gcggtgataa ggtcgtctgg gaggacaacc
     6721 ggaccgggaa ccgggatatc tacctctacg acctcgctac aggagaggag acgcagatca
     6781 cgaccgggac cggccacgag atcaaccccg ccatcgccgg ggactggatc gtctggcagg
     6841 acgcacggag tggcaactgg gacatctacc tctacgacct cgctacggga gaggagaccc
     6901 ggatcaccac aaacgagagc acacagatga ctcccgctgt caccacgagt atggtcgtct
     6961 ggaacgactt ccggcacggc aactgggaca tctacgcctg gattcccgat gaagcggtgc
     7021 cgtccgtgcc cggaccggcc ctgacgcccg cctcgcccgg agcgcccggt gcagtcactc
     7081 tgccgccgac cgcaatgccg acggcaacac ccacgccagc cgcaacgccc ccgacgaatg
     7141 taacggaaac gctgacgccg gagggtacgc cgacaaccca gccaccgaga accttgccac
     7201 cggggacctt cccaccgttg accttcgtaa ccctggaacc aaccgagaca cagacaccgt
     7261 tcctcctgtg acggaaacag gacttaacgg tgaaactccg gtatacctgc cggctccacg
     7321 gagcatgccg tgaggctgtc ccggagatcc cggagagagg cggttcaccc tcgctccggg
     7381 actttcagtc ccgtcaaact cctgcgtctt gcaaggtgca tccagcaggt gagattcggg
     7441 atcgacaatt cctccggcga ccgaaagcct cgcgaagccg ttatgaacgc cgaaccggta
     7501 gcttccggct aacgcatccg ttcgccgggg tgaaaaatga aaaagggagg gtattcgatt
     7561 cgcgttacgc cagataatcg tccggccgcg ggatctgttc ctttaagccc ttccgcttgc
     7621 ggatggcctt gaccacatcg gtcacaatac cggcggggac cagttcgaac ccggcgaact
     7681 cggtgctcca catcgcgcgc ccttcggtgg cggaccggat gtctccggca aacccgaaga
     7741 gctcggcgac cggtgccctg ccgacgatcg tcatcgtatc cccttcgctc agcatatcga
     7801 agacctggcc gcgccggccc tggatctgcg aagttgccgc acccatctgc tcggtcggga
     7861 ccgtgatctg gagcttctgg ataggctcga gaagcgagtc gccgccgagc agcaggcctg
     7921 ccttgacggc actcctgact gccgggatga cctgcgcggg accgcggtgg atggcatcct
     7981 cgtggagttt cacatccacc agccggatct tcaagttctg caccagctcg tcggcgagcg
     8041 ggccgccgcg gagcgcttca cgccagccgt cgaggaccag ttccatcgtc tcgttgaggt
     8101 actggacacc cttcgtcatg tcgatgaaca ggttggtacc ctcgatcgcc ctgaggttct
     8161 tggcctcgtc cttgtccata ccggcggcaa cgagggcatc acgccgctcg atcgcctgct
     8221 gggtcatcga tatctcgccg tcccggatca tcttcacgat cgcggggtcg accggctcaa
     8281 gctcaaggta gaaccggttg tggcggttcg gcgactttcc ttcgaccggg cctgcccgac
     8341 ccgtgatcgt ctcgcggtag accacgatcg gcggggaggt gatgatatcg acacccttat
     8401 cacgcttgat acgaccggtg atgatctcga gatggagttc gcccatgccg ctgatcaggt
     8461 gctcgccggt ctcctcattg atcgagaccc gcaccgtcgg gtcttcctta cccacctggc
     8521 ggagaacctc gacgagtttc gggagatcct tcatgctctt cgcctcgaca gcgacggtca
     8581 tgaccggctc ggagtagtgc ttcagcgact cgaagggggc catttcaatg agcgacgtga
     8641 cggtcgagcc gacgatggcg tcctttaacc ctgtcacggc ggcgatgttg ccggcggtca
     8701 gcccttccac ctcaatccgc tcggcaccca tgaagatgcc gacctgagcg agacggttcg
     8761 aacgctttgc cgcacccatg atgtagcact cggtgccccg gcggagcgtt cccgagaaga
     8821 gacggccggt ggcgacctcg cctgcatgcg ggtcgaacga tatatcggtg accatcaggc
     8881 agacgggccc gttcgggtcg cagttgagca ttgccttacc ctcgggggag gtatagtcgc
     8941 cgcgccagat gatgtggata cgccggtcct gggcctggag ggggttgggg aggtgcttga
     9001 ccaccatgtc gaggaggacg gcatgcaggg ggcttttctt cgccagccac ttcatgtcgc
     9061 cggtattgca cttctcatag acgtccttga acgagacccc gctgctcttc atgaaagggg
     9121 cggatacggc ccagttgtag agcgcggaac cgaaggcaac ggtgcccttc gcggcatcca
     9181 gtttccagcc ttcgttgtac atcttctcgt tcatgccctt gatcaacttg ttgaccttgt
     9241 cgatcacctt cgcgaggcgg atctgcatct cctgctcgtc caccttcagc tcgttgatca
     9301 gccggtccac cttgttgatg aagagaaccg ggcggacacc ctccttcaag gcctgacgga
     9361 gcaccgtctc cgtctggggc atggtcccct cgacggcgtc gaccacgacg acggcaccgt
     9421 ccaccgcacg catggcgcgg gtcacgtcgc cgccgaagtc cacgtggccg ggggtatcga
     9481 tcatgttgat caggtactct tcgccgccgt actcgtggac catcgagacg ttgctcgcat
     9541 cgatggtgat gccgcgtgcc tgttcctcct cgtcggagtc catgaagagc tgccggccgg
     9601 cgagctcctc gctgatcatg cccgctcctg ccagcaggtt gtccgagagt gttgtcttac
     9661 cgtgatcgat gtgggcgacg ataccgatgt tccggatgtg ctccggcttg tccatcagct
     9721 ccgtcacccg ttctaccatc ttctttcgtc tggtcatgaa ttacaccaaa aaaaataata
     9781 gggggtttta acgggctgcc tttgcaatac gttctctctc ttctttcttc gagacggcgt
     9841 atgaacgggt gtcgccgttc gccgcagata tcagctcttc ggcaagcgcc tcgctcacgc
     9901 tcttcttctt cttgtggctc gcctgccgaa cgccggcagt gatgaaccgg agcgcagtat
     9961 cgacacggcg ctggggagcg gtatcgaccg acttcgggac gttgatgccg ccgtacttca
    10021 gccggacggt ctcctcacgg ggtccggtgt tcgcaactgc gtccaccagc acctgaaccg
    10081 ggttcttctt ggtcttcttg ttgacgatct cgaaggcgtc ccggacgatg cggatggcaa
    10141 gttccttctt gccggtgttc ttctcggtct gcatcagttt gttgatcaga cgctcgacga
    10201 tcagcatgtt gctcttgttg aactgctggc cgacgagttt gccgcaggag tgcggcacaa
    10261 tcatcgtgtg caggttcacg taacgagcga ggctcggatc caggatctcc acctcggtca
    10321 tgtcccagcg gttaaaaagg agctgctgcg cccctttctc ttccggctgc gtagttcctg
    10381 cttctgcttc taccatactg cttacctccg cggcttctcc ttgcgtccgg taaccatttc
    10441 acgcaggctg acgctgttca ccttggtcac gacaaaccgg acgccgggga tatcaccctt
    10501 cgacctgccc agcctgccgc cgatgccctc gatctcgacc tcatcgtgct cgtcgatgaa
    10561 gttgatggca ccgtcgccga cagcgaacgc ggttacctga cggccgttct tgatcagctg
    10621 tacgcggacg cacttgcgga tcgcagagtt cggctgcttt gcctcaacac ccaccttctc
    10681 gagaacgatc ccgcggccct gcggtgctcc ctcaaggggg tcagnngtta ttatttgcgt
    10741 cgcgcttcag ttttcgggct gcaaattttc catttcccat cgaataccct cgtatcagtt
    10801 gaatttgtgg ataggagcaa ctcctggtgt gctataaagc aacgcgcgtt acggatttat
    10861 atattgtgga acccgcccgc gcatcgatct tccgccttag ttatttgatc gggagctgat
    10921 ataatattat atcctgacgg gcaaccttca ttgatgataa cccggatata caaagaggtt
    10981 tttttcgagg caacccatcg cctgattcac tatcagggga agtgtttccg gctgcacggt
    11041 caccagtggc gggtagaggt ctggatcgag gggatcgcag atgagcgcac cggcatcgtc
    11101 cttgactaca actgcatcaa agaagtcgtc gggcggttcg atcaccaggt gatcttaaac
    11161 gccgacgatc ctatggcggc aagcatcgag gcgttccacc cggtcgtcac cacccccggc
    11221 gacccgacga gcgagctcct gaccggcctc atcgcagaga tgatcgatgc tgaagccgca
    11281 cggcagggac acgatgcccg cgtggcgaag gtccgggtct gggagtcgac gtcctgttac
    11341 gcagagcgca cctatgatcg tcagtgagat cttccggagc ctccaggggg agggaaagag
    11401 tcaggggcgg ccctgcacgt tcatccggct caccgggtgc aacctccggt gcgcctggtg
    11461 cgacacctcc tacgcccggg aaggcgggga ggagatgagc atcacagagg tcctcgaccg
    11521 ggtctggctg cagaacggga agcagatctg catcaccggc ggggaacccc tcctgcagca
    11581 ggaagcggtt ctcgaactcc tcaagaagtt cagccttcac gggtataccg tcgagatcga
    11641 gacgaacggc acccgggact tccagaagat gcagccgtac gctaccatct gcatggatgt
    11701 caagtgcccc tcgtcgggcg agaggagcga tctccggctc ctccccttca tcaccccccg
    11761 cgactgcgtc aaattcgtgg tggcggacga ggacgacctc ctctacgccc gggcggtgat
    11821 ggcccgttac gacatccggg gcgagatctt cgtctcgccg gtggaagggt cggactaccg
    11881 cgccatcgcc gactacatcg tcgaggagaa cctcccggtg aggttccagt tgcaactaca
    11941 caagatcctg ggagtaaaat gatgaaagca gtctgtcttc tctcgggcgg catggactcc
    12001 accacgctcg cttacgtcgc aaaggatatg ggttacgaga tcgtcgcgct ccacttcacc
    12061 tacggccaga gaaccgagga gaaggagcgc tcatgcgccc ggacagtcgc ccggatgctc
    12121 ggcgcgacgg atttcgtcga gatcagcctc gatcacttca ggaagatcgg ggcctcgagc
    12181 ctgacggatg agagcatccc cgtcgaagag tacgccggtg aggaggaggg ggtgccgagc
    12241 acctacgtcc ccttccggaa cgcgaacctc ctcgccatcg cgaccagcct cgcggaagcc
    12301 aggcgggctg aggcgatctt catcggcgtc cagaccgggg actacccggg gtatcccgac
    12361 tgccggccgg agttcatcga ggcgttccag cgggcggtcg acctcggcac ggcggcggac
    12421 ccccggatca cccttatgac gcccttcgtc cggatgacga aggtggacat catcaaaaag
    12481 ggccttctcc tcggcgtccc ctatgagcag acctggtcct gctaccagaa caatgaccgg
    12541 gcatgcgggg tctgctcctc ctgccactac aggagagagg cgttccggga gctcgggctg
    12601 aaggacccga tcgagtacga gaggtaacta tggagatcta ccgcggcaga cggcttaccg
    12661 tcgagaagaa ggagattacc ctctcaaacg gggagaagaa ggagcgggtg gtcatccacc
    12721 ccggcggggc ggtcgcgatc ctccccgtcg tcggggatga ctgctacctc atccggcagt
    12781 accgcttcgc catcgacgag tacatcttcg aggcgccggc aggcaccatc gacgagggcg
    12841 aggagcccca cgaaaccgcc caccgggaac tgatcgaaga gaccgggatg aaggcggaga
    12901 cgttcgtccc gaaaggctac atctacccct ctcccggcta caccgacgag cggatctggc
    12961 tctaccgggc cgagggcctc tcgccgtgct ccgactacgg gatggacgac gacgagatga
    13021 tcgaggtcgt ccgtgtccct atcgcggacg tcgggtcgat gatcgcggac ggcaggatcg
    13081 tcgatgcaaa gacgatctgc ctcatctgcc ggtgcctgtg gtgagggggc acgccggggc
    13141 cggcggatgt gcgcagattt atgcccttcc tcagagaaac atatagtagc ttcgacaatt
    13201 gaacaacatt cagctggcct ggtgaacgtc caatggaaga gacgcaagag atggatacgc
    13261 cgcgaaagcg ctacgaattt aagaagatgc ttgagaggct tgcggagaag gaagggagcg
    13321 gcaccgagct gatcaccctg tatatacctc cggataagca gatatacgac gtgaccgccc
    13381 agctccgcga cgagttcggg cagtgcgcca acatcaagag taagcagacc cgaacaaacg
    13441 tccagagtgc catatcctcc atcctctccc gcctgaaata ctataagcgc ccgccggcaa
    13501 acggcatagc ggtcttctgc ggcacgatca acatcggcgg cgaccgcacc gaccttgact
    13561 gcgagatcat cgagccgccc gaaccgctca acacctacat gtaccggtgc agctcctcgt
    13621 tcgaactcga accgctgcag cagatgctcg gggagaagga ggtctacggc ctgatcgtcc
    13681 tcgaccggcg agaggcctac atcggcttcc tgcgaggcaa ccgtatcgag ccggtccacg
    13741 gcatcacctc gacggtcccc ggcaagcagc gaaaaggtgg tcagtcgagc gcacgtttcc
    13801 agcggctgcg gctgatcgcc atcaacgagt tctacaagaa gatcggcgat catgcaagcg
    13861 agatcttcct cgccgagaag gatttcttcg agcggttcaa gggcgtgctg ataggaggcc
    13921 cgacgccgac caaggaggag ttcgaggcgg gtagtttcct ccaccacgaa ctgcagaagc
    13981 ggatcctcgg cctcttcgac gtctgctaca cgaatgagtc agggcttgcc gaactcgtcg
    14041 agagtgcgtc ggacgccttg aaggaccttg atgtcatcaa ggagaagaac gtgatgaacc
    14101 ggttcctcca ggaactcgtc aaagatgacg gcgctgcctc ctacggcgag gagagcgtcc
    14161 ggaagaacct ggagatcggc gccgtcgaca ccctcctcct ctccgagaaa ctccggaggg
    14221 ccaggctgaa acttgcctgc cagaataacg actacaccga ggagcggacg gtcagcattg
    14281 agccgggcaa acatatcaag gacctcgact tcggcacctg cccaaacgac ggcgggtcgc
    14341 tctacgtgac ggaagaggtc gatatcatcg acgaactgac cagacttgca gaccagagca
    14401 gcgcgacggt tcggattata tcggacgact ttgaagaagg ctctatgctc tacaacgcat
    14461 tcggcgggat cgcagcgatc ctgcgttaca ggaccggata ctgatgtttc aggagaaata
    14521 ccaacagatt gagcgcatgc tccgggcatg caccggtgaa gaggatgtcc tcctcacgac
    14581 agggggggat cacgccgatc tcgcctcgac cgtagcgttt aaacttgcga agagagagaa
    14641 gcgggcaccc gccatgatag ccgccgatat caccggaaaa ctctcggcaa accccgatct
    14701 cggcgatatc caggtcgagg cgacgggccc ttacgtcaac ttcaggttcg ggccgtcact
    14761 cctttccgag gcggtcaggg aggccgttcg ccccggctac ggaagcctcc cccaccgctc
    14821 cgggcgggtc gtgctcgagc acacgagcgc gaacccgaac ggccccctcc atgtcggcca
    14881 catcaggaac accgtcatcg gggacactct cgcccgggcg tttcggaagg ccggctatcc
    14941 gctcgaggtc cagtattact taaacgacat gggccgccag atcgccatcg tctcgtgggg
    15001 gttcgaccac ctcggtctcg cccgcgagga gggtgagaag gaggaccact acgtcgcccg
    15061 tgtctacatc gcggcgaacc aggaactcca gaaggacccg gagatcacaa aagagatcga
    15121 ccacctcatg cagaaggtgg agcgcggcga tgcggagacc gtcgcaaagt tcaggaaagc
    15181 cgtctccctc tgtgcagagg ggatcaaggc gacccttgcc ggcttaaacg cccaccacga
    15241 ccggtttgtc tgggagagcg attttgtccg gatcggcgac gtggaccgga tcatggaacg
    15301 ggtccggcgc ctgtcgcagg cccacgagga cgagaccctc tgggtcgacc tcacggagca
    15361 gggtttcgag aagaagtaca tcctccggcg gagcgacggc acctcggtct acagcacccg
    15421 cgacctcgcc taccacacct ggaaggggcg caactacgac cggatgatcg atgtcctcgg
    15481 ggcggaccac aaactgatcg gcgcccagct ccaggccacc ctcaaactcc tcggcgaaca
    15541 gccgcccgag atcgtcttct tcgagttcgt ctccctcccc gagggctcca tgagcacccg
    15601 ggcaggcaag ttcgtttcgg cggacgcgct gatggaggag gtgaccgcga aggcgttcga
    15661 cgaagtgacc gtccgccgcc cggaactccc cctggaggag cggcgctcaa tcgcaaagtc
    15721 ggtcgccatc gccgccatcc gctacgacat cgtgaaggtc tcccccgaga agagcacggt
    15781 ctttaactgg aaagaggcgc tggatttcga gcggcagagc ggcccctaca tccagtacgc
    15841 ccacgcccgc gcctgcagca tcctggagaa ggccggagag ttcgagcagg tgtaccgcta
    15901 cgagaccgaa cacgagatcg cgctcgcccg ccacctcgcg aggttccccg ctgctatcga
    15961 gcagaccata gaggaactcc gcccgcacct gctcgcctcc tacgcccgcg agctcgccga
    16021 cctcttcaac gccttctacc actacgaccc ggtcttgaaa agcgagggcg agacacggag
    16081 cagccgcctt acgctcgtgg acgcggtcag gaacacctta aaagagtctc tggagatact
    16141 tggtatcgat gcactcagaa gcatgtaagg ccctcaaaaa gcagggctac cagttcatca
    16201 gcccgaactc ttcggcggcc gtcaaaccct gcatgtggaa caagcgggcg ctcaaaggcg
    16261 gggagatgtg ctacaaagcg cagttctacg gcatcgagag ccaccgctgc gtccagatga
    16321 ccccgaccct ccggtgcaac cagcagtgtc tcttctgctg gcggtcgttc gagcacgagg
    16381 tgacggaaga ggtggagtgc ccgccgtccg cgatcatcga aaacctccgg cgcgtccaga
    16441 agagggcgct ctcgggctac aaggtctcgc cctacgtcac accggagcga ttcgccgagg
    16501 ccctcgaccc cacgatggtc gcgatatcgc tctcggggga gccgacctgc tactcgcacc
    16561 tcccggaact gatcgacggc ctcaacactg aagggtatac gacgttcctc gtctcgaacg
    16621 gcacccggcc cgaagtcctc gcccggtgcc ggccctacca gacctacgtc tccctggacg
    16681 ctcccgaccg cgagacctac ctccacctct gccggccccg ggaggactac tgggaccgga
    16741 tccacgagag cctcgccgga cttgccg
//
LOCUS       DAJB01000019            4095 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_123306, whole genome shotgun sequence.
ACCESSION   DAJB01000019 DAJB01000000
VERSION     DAJB01000019.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_123306"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          723 a         1283 c         1299 g          790 t
ORIGIN      
        1 gagggatacc ttcgaagtcg tagatcgccg agaccggtac cgccatcagg acaaatgcga
       61 taagcatgca taaccacgtg tgaatatttt tgaatatgtt catactatct atcaaaagat
      121 aacatctggt attaatatat tttattttat tcttactaat ggggagggct cttttgcctc
      181 gttatggggc agggcaattt gcaaacctca ctcacccgac agcgttgccc cgggtctgtt
      241 actatccctg aaattttccg gaggtacccg aggctctcct gcaaaccggg gtgcgcctga
      301 aggagtggtg ggcgcgtaag accggaggtg ggagcggatg ccccggcgat tgcacccccg
      361 cgaccggtgc agggcctcgc cggcgctccg ggaaagctca tcggagttat taattttagc
      421 ctgtttgtta acactcaatt gttcaaacct aaaatttgcc ctgaattagt aacacaaacg
      481 ataaaatatt taatacaaaa cgataagatt catgaagtga caccgaacag agcattactt
      541 ctgtgcatcc tggtcttcgt gcttgtggcc cctgcaacag caggcagtgt cgaaaggacc
      601 gtcgctcaac tgccggaagg cgccgtgggc gatctggagg tgacgctcac ggtgacggat
      661 atcccggtcg ggggcatcgt cgagaccatc cccgacggct gcacatgggc cgggtgcgac
      721 cacccggacg accgcacaag ggtctcgaac cggacggttg ccttcgccgt tatcggcgaa
      781 gaaacgatcc gctaccggct gcaggggccg ccggaggccg gagtagcgct aaccgggacg
      841 tgggaaggtc tgctgaccgg tgcatcgggc gtcgtggggt cggacgaacc ccgggattcc
      901 ggacaggcca cgcagacaac ggtcccgggc acgcccggtt tcgggtatgc agccgcgctg
      961 gcggcacttg ccgtagtatt cgggaggagg tgcggccggt gacccgacga ctcctcctgc
     1021 tcgtctgctg catctgcctc ctggccgggg cggcagcggc ggaaactatt ccgtgcgaca
     1081 gcgacggcga cggcagactg acctcctcgg aactcgcgac cgcgatcctc gactcgctgg
     1141 acgcccggtt catgggcgga accgtcgacg ccccatcttc cggcgacctc cgggacgctg
     1201 cgttcgtcta cgagcactgg gacggcaggg tgctcacgat caccgattcg tccggacggg
     1261 cgacgacact cacccgcccg ctccggagga tcgcggtctt taacagcgac accctcgaga
     1321 tgatgcggtc gatcggcata gagtccgatc gggtcgtggg tgtgagcaag tacacccttg
     1381 aggacccgac atacttccct gagtaccgcg agacggcgaa ccttggatcg gtctggtccc
     1441 ccgactacga gcaggcggct gcggttcgcc ccgatgcggt cttcctctac gccactatct
     1501 cccagtcgtc ctgtgacgat atcgaagcga cactcggggc catcgacccc ggcatccgct
     1561 tcttccggtt cgacggttac ctgccgacgg tctacgccga cgaggtgcgg acgctcggtc
     1621 tcctcctcgg gaaagaagag gaggccggga ggttcctcgc gttctacgga aacgttacgg
     1681 ataccgtggc aggagtggtc gacccaatcc cggccgacga ccgggtaccg gtctacctgg
     1741 agagctggaa cgactacaag agcgccggaa agggctcggg ctacgacgag aagatcaaac
     1801 ttgccggcgg caggaacatc tttgccgaca ccgcggtcga atacccggtg gtcgaccccg
     1861 aggcggtcat cagcaggaac ccgggcgtca tcgtcaagat cgtcggcgcc ggcgaactgg
     1921 tgttcggcgg ctacggcgac gacgacccgt cgtccttcga gacggtctac cgcgcaattg
     1981 gggatcgccc ggtctgggac cggatcggtg cggtcaggga cgatcgggtg catatcatcc
     2041 actccgacgt catcgggggg cccgaatact tcatcggcgt cgcgtacatg gcgaaatggt
     2101 tctacccgga cctcttcccc gacctggacc cccgtgccat tcaccggcag tatctggagg
     2161 agttccagcg gctcgactac gacctcgacg aacacgggac gtttgtgtac ccggcatgat
     2221 gaccgacttc gggaggcagg agtacgaaag gctcacggag aagagggcgc tcttcatcac
     2281 gggccttgtc ctgatcctcg tggcgctcgt cgggatcgcc gtgaccctcg ggtcggcgga
     2341 cctcccggtc gccgactcct acctcgctat cttcgccggg ctcttccccg gggcggtcga
     2401 cctgccggaa cggatgatcg agtccaacgc ggtcgggatc gtctggggct ggcggctgca
     2461 ccgcgtgctc ttcgcgatcg tggcaggatt cgggcttgcg atcgccggca cggtgatgca
     2521 gggtgtcctc agaaacccgc tcgcaagccc tttcacgctc gggatcgcct ccgccgcctc
     2581 gtgcggcgca tcgatcgcca tcgttctcgg ggccggtatt ctctccgggg gctacctcat
     2641 cgtcggaaac gccttcctct ttgccatgct cgctgccggt gccatctacg gcatggcccg
     2701 cctgcggggc atgggaagcg agacgatgat cctcgccggc atcgcgctca tgtacctctt
     2761 ctcggccgtc acctcactcc tccagtacct ggggacctcc gaaaaggtgc aggagatcgt
     2821 cttctggatg ttcgggtccc tggacaagtc atcctggccg aaactggcga tcgtgacggt
     2881 cgtcgtcgct gccgtcgtcc cacccctcct ctggcgcgcg tgggacctca acgcgctcgc
     2941 cgaaggcgac gagacggcga agagcctcgg cgtgccggtg gagcgatcca tggcctggtt
     3001 catggtcgcc gcatcgctcg taacggcggt gatcatctgc ttcaccggca ccatcgggtt
     3061 catcggcctg gtcgcgcccc atatcacccg gatggcgatc ggggccgacc accgcaccct
     3121 cctccccgcg tccgggctcg tgggcgccgt cctcctcctc ggcgccgact gcctcgcacg
     3181 cacggcaatc cccgggtcga tcgtcccggt cgggatcatg acggcgttcc tcggcatccc
     3241 gttcttcctc tatctcttca tgcgcaggag ggacgcctga tgacgcccct gctctgtgca
     3301 cgcgggctct cgttttcgta ccgcgaccgg caggtgcttg cggacgtctc gttctccctt
     3361 gacagggggg aagtcctcgg gcttgtcggg ccgaacggat ccggaaaaac gacgctgctc
     3421 cggtgcctgg accggattct cgacccggaa ggagcggtct tcctcgatgg gagggagatg
     3481 tgctccatga accggccgga gatcgcgcgg gcggtcgcct acgtcccgca gaacggcggt
     3541 gctctgggtt ctgcgacggt ctttgagacc gtcctgatgg gcaggcggcc gcacctgcgc
     3601 tggagcgtct caaaagagga taaacagaag gtctcggagg cgctctccct tctcgatatc
     3661 ggtgatttcg cgtcccgcag ggccgaccgg ctctcgggag gagagcggca gcgggtgctg
     3721 atcgcaaggg cgctcgtgca ggatgcccgg ctcattcttc ttgacgaacc gacaagcgcg
     3781 cttgacatgc gcaaccagat cgaggtgatg gacctcctgc gccggcttgc ggaagaacac
     3841 cggcttgcgg tggtggcggc gatccatgac ctgaaccttg ccgcccgcta ctgtcaccgg
     3901 atcatgatgc tgaaggaggg gcgggtgctc ggacaggggg cgccgtcttc cctcctgacc
     3961 ggaggaatga tgcgcgcagt ctatggtatc gaggcggtcg tgaaggacga gatgggggtt
     4021 ccgtacgtgg ttcctctccg gtcgttggcg aatagcaaca caatggagat cgtgtcaact
     4081 acccccggtt gaaac
//
LOCUS       DAJB01000020            3268 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_72522, whole genome shotgun sequence.
ACCESSION   DAJB01000020 DAJB01000000
VERSION     DAJB01000020.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_72522"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          704 a         1010 c          973 g          581 t
ORIGIN      
        1 gatgcgggca attcctcccc cggttaaaac cgggggtctc cttgcccgac gtacttatga
       61 tgatgctcaa gcgcttcctc tacctggcag aggaggggag ttattccacc ggggaggtcc
      121 cggatacggt tgtcggcatc gcgctctcgg gtgtcccgct cgccaccctc atcgccgcgg
      181 aggaggggct gaaactcgcg gtttacctcc cggcgaaaca cagtcggagc gagactccta
      241 ccggatccct ctcgggcacc ttctccacga tcaccgctca gcgctgcatc atcatcgacg
      301 acgtcgtcac gaccgggacg acgctcgcgg aggcaatagc gttcctccgg cggcatggag
      361 ccacgccggt ggcagtctgg tcgctcttcg acaagcaggg ggtccgggag atcgacggcg
      421 tccctatcca ctcgctcttc gcgatatcga ggctcgggtg agggcttcta tctcacgtct
      481 ttttttgaat actcccggag cagggcgatg cgaccggtgc gcccgccagg ggtaccaggc
      541 gcgtagcgtc aaccagaagg cgatccgccc ccgccgttcg gcccggccgc cccgggagaa
      601 ccggacccta tcgactaata tatatagaac ctctgaacaa atctaacgga caaaggagga
      661 ttatacaatg cttgctggac agcccatcat tattttacgg gaaaatgttg agcgcacgcg
      721 cggattcgag gcgcagcgct cgaatatcat ggctgcaaag gcgattgcgg ccgccgttcg
      781 gacaacgctc ggtccccggg gaatggacaa gatgctggtc agctccagcg gtgatgtggt
      841 gatcacgaac gacggggcaa ccatcctgca cgagatgtcc gtgcagcacc ccggcgccaa
      901 gctggtcgtc gaggttgcag agacccagga cggcgaggtc ggcgacggca ccacaaccgc
      961 gaccgtactc gtcgggtcgc tgatggaaga ggcagagaga ctgctcgccc aggaggttca
     1021 tcccacgatc attgcgaacg gctaccagct cggcatggag aaggcccttc tgctcctcga
     1081 cgacctggcc atcaccgtcg ggttcggcga ccgcggcgac ctcctgaaga tcgccgacac
     1141 cgccatgacc ggcaaatcga tcgaagcggt caaggataag atctccggca tcgtggtgga
     1201 cgcggtgcgg caggtcgccg tcgagagcgc ccggggcacc tacaccgtgg acgaagacga
     1261 cgtcaagatc aagacgcagg tcggcgactc gatgaacgac gccaaactga tccggggagt
     1321 cgtcatcgac aagaagcgcg tctccgagca gatgcccacc aaggtcgcgg acgctaaagt
     1381 agccctgctt gccctgccgc tcgaggtcac gaagacccag gttaagtcga agatcaagat
     1441 cacctcctcc gaccagatga aggcgttctc cgagcaggag cgcgagaccc tccggaaact
     1501 tgccgaccag atcgttgcct ccggcgcgaa cgtcgtcctc tgccagaagg ggatcgccga
     1561 cgcggtgcag ttctacctgg ccaaagccgg gatttacgcc attgaagacg tcaaagaggg
     1621 cgacatgaag ttcgccgcca aggcgctctg cggcaccatc gtcaacaagc ccgaagaact
     1681 caatgcagcg acgctcggcc acgcggaagc gacccgggag gtccctgatg ccgacctgac
     1741 gatcatctcc ggctgcgaca acccgaaagc cgtcacgatc ctcctgcggg ggacatccca
     1801 gctgctgctg gacgaactcg agcgggcggt ctacgacgcg gcccgggtcg tccaggacgc
     1861 cattgaggac ggtaagttcg ttgtcggcgg cggttcggtg gagactgagc ttcaactccg
     1921 catgcgcgag tacgccgcaa ccatcggcgg gcgggtccag cttgccatcg aggcgttcgc
     1981 aaacgcgttc gaggtcatcc cgaggacgct cgcagagaac tccgggttcg acacgatcga
     2041 caaggtcgtc gccctgagga aggcccacgc tgacggcgcg aagtacgccg ggctcaacgt
     2101 ctacaccggc gaggtcgtgg atatgcggga ggccggcgtc atcgagcccc tgcgggtgaa
     2161 gacccaggcg atcaagagcg cgaccgagac ggccatgctc ctcgtccgcg tcgacgacat
     2221 gatggtcacc cagtccggaa gaccgggccc acaataaatc acccgcgtga ggaggtcagg
     2281 aagacgccga cctcctcttc ctcgattttt ttatcctgcg gcaggcttct tccgtgatga
     2341 aagaccaacg ctgcgtccgg gttgaacccg aaggagagga gagcggtctc ataggtatcg
     2401 cccggccgcc cccggtagat cagcacgttg ccgtccggga tgagggtgaa tcgacagagc
     2461 atatctcgat actattatag tacaggaacc taaatactct aggctgattc tgtgccgagg
     2521 tagtctagtc cgggaaggcg gtggcctcga aagccactgg tgcctggcac ctcgggagtt
     2581 caaatctccc cctcggcgtt cattttgata gttcaggtcc ccatgggttc tatgcacgag
     2641 catgagcccg gaggccccgt tctattcgca caacgcgcga gccaatcccg atcgcgttgt
     2701 gcagagcgat ttttgaaggc cggctcatcg aggagtacat cgccgggcag caggcgctct
     2761 gccggatcaa cgctcaccgg gtacgaaaag accatctacg acccgatctc gtggcgcagg
     2821 ttcatccggt cgttttggga gattacggcc gggaacctgt atgccggcct gaacaagatg
     2881 cggacgggca cgagcgcacg cggcacaccc ccatgacaga tgtaaaaatc cccgacaggc
     2941 ggcatttgaa agggatccct cacggttcaa aaaagacggg ataagcgtat aggatatcaa
     3001 cgtattgccc ctgggctgag gagcagcccc tttcaagccc ccccacacga cctatccacc
     3061 ggttgaattt cctgcacaat ttttcgcgac agatagccgg cgtaaatatc aggacatata
     3121 aagatccccg tcaatctccc gttttcttcc ataaccgata gagattcccg gcggtcggcc
     3181 atcaacaaca atttcatcga gagtccgata ttctctctct cgtcatgatg aaagagggat
     3241 gactcgatcc caaactattc cctcactt
//
LOCUS       DAJB01000021            6923 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_58561, whole genome shotgun sequence.
ACCESSION   DAJB01000021 DAJB01000000
VERSION     DAJB01000021.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_58561"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    201..231
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3645..3670
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1349 a         2013 c         2172 g         1332 t
ORIGIN      
        1 gcaccgggtg ctatcgggat ccggacgaga gcaggaaagg gatgcacccg ttaccctccg
       61 ggatcacaac catgaatgag agaggcggga ccaggcgaac agtacagcag gagaggaggc
      121 ggataattcc atggatatcg gtgtgatcgg catcggggtc atggggagaa accatgtccg
      181 ggtctattca gagctcaaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccggcgtc
      241 cacctgctga tggagaaacc gatctgtgcg accaccaggg aggcacagga gctcctcgcc
      301 ctccgcagcg aggaccaggt cttcggggtc ggccacatcg agcgcttcaa tcccatcgtc
      361 cccgagatac gccggatcat acgcaaaccc ctcttcgtgg agatgaaccg gcacaacccg
      421 gcatccgcac gggcggtcga ggcatcggtc atcgaggacc tgatgatcca tgacctcgac
      481 atcctccagc acgtcctctt cggtcacccg ggcgagctct ctgccgcggg gagcctggat
      541 gccgcgagcg ccctcttccg gttcaacacc accgcagcct tcatctcggc gagccgcaag
      601 tcctcgaaga agatccggcg catctacatc gaggaggagg acctgaccgt ggagggcgac
      661 ttcatgaacc aggaagtctt cacctactac aagcccggga agtacggggt ggagaacgaa
      721 cggtacgtgc aggagaacat catcgagaag gtgctcgtga acaagcagga gccgctgaaa
      781 ctcgagctct cgacgttcct cgactgcgcg gccagaggaa gggagttccc ggtcagcccc
      841 gcacaggcgc tgctgaacat ggagatctgc gaggatgtcg cgcggtgttt ctcgacgtaa
      901 ggatggtttt tcatgagtag caggttacag tcgatcatcg acacgagagg accgatccgc
      961 accgtcggtg tgatcgggat gggctacgtc ggcatccctg ccgcggtgct ctttgcgaac
     1021 gctcccgggt tcgagtcggt ccggggattc cagcgagact ctccgtcctc cggctacaag
     1081 gtcgctatgc tcaaccgggg ggagtcaccg ctcaagggtg aggagccggg tctcgaagac
     1141 ctcctcggca gggtcgtcgg cgccgggaag ttccggtgca cctcggattt ttcacagatc
     1201 gcagagtgcg atgcggtaac ccttgccatc cagacaccgt ttaaagatcc caaagacctg
     1261 atccccgact tctcggccct gatcgagggg ctccgcgcgg cggggagaca cctctcggag
     1321 gggacgctcg tggtgctgga gtcgacggtc acccccggca cgaccaccgg aatggcccgg
     1381 gagatccttg aggaagagtc gggactcgtc gccggcgagg acttctgcct cgcgcacgct
     1441 cccgagcggg tgatggtcgg gcggctgctc cggaatatcc gggagcacga ccggatcgtc
     1501 ggcgggatcg acgacgtctc gacccggcgg gcggtggaac tctactcgcc cgtcctcacc
     1561 acggggaaga tcatcccgat gaccgcgacc gctgccgagg tgacgaagac cgccgagaac
     1621 gccttccggg acctccagat cgccgctgca aaccagcttg ccctccattg cgaggcgatg
     1681 ggcgtcaacg tctatgacgt ccgggcgggg atcgactcgc tcaagggcgc cgggatcacc
     1741 cgggccatcc tctggccggg cgccggggtc ggcggccact gtctcacgaa ggactcgtgg
     1801 cacctcgagc ggggtgcaca ggtcctcggg catggcgacc tctggtaccc gcacggggcg
     1861 gagtcgatct ttggcgtcgc acggaagatc aacgagttca tgccccggca catggtccac
     1921 ctgaccctcg aggggctcaa gcggacgggt aagtccccgg agggcgcgaa gatcgcactc
     1981 ctcgggtggg cgttcattca gaactcggat gataccagga acacgccggc ggagccctat
     2041 ctcgcggcga tggaggagac gggtgccgtg gtcagggtcc acgacccgtt cgtgaacgac
     2101 tatccgggta tcgaggtctc gcacgacctc gacgagaccc tcgcgggagc ggatgtcgtc
     2161 accgtcttca cggctcatca ccactacgct tccctcgacc cggtccgggt aaaggaactc
     2221 tcgggccggg agcacccggt gatcgtcgac ggcagaaacg tcgtcgaccc ggacgcgttc
     2281 atccgggcgg gtttcatcta caagggtatc ggccggggcg acaggaacag ccacccggtc
     2341 gctccgtaac ggtgtaccat gaagatcgtc tccatcgtcg gcgcccggcc gcagttcatc
     2401 aagtgcgctc ccgtctcccg ggaactcagg aaggagcacg aggaggtcct cgtccacacc
     2461 ggtcagcact acgaccacgg catgtcggag gttttcttcg aggaactcgc tatcccgaag
     2521 cccgactaca acctcaatat aggctccggc acccacggcc accagaccgg gacgatgctc
     2581 ggggcgatcg aggacgtcct cgaagtggag aagcccgacg tcgttttagt ctacggcgac
     2641 acgaattcca ccctcgcagg cgcgcttgcg gcggcaaaac tccacgtacc ggtggcgcac
     2701 atcgaggcgg ggctccggag ctttgatcgc cggatgcccg aagaggtgaa ccgggtgctc
     2761 actgaccact gctcggacat gctcttctgc cccacagaga cggccgtccg gaacctcgcg
     2821 gccgaagggg tcacggaggg cgttttcctc gtcggggacg tgatgtgcga cgcgatggag
     2881 tacaaccgcg cggttgctga agagcgctcc cgggttctcg aagaggttgg ggtcaggccg
     2941 ggggagtacc tcgtcctcac cgtccaccgg ccctcgaaca ctgacagcag ggagaatatg
     3001 gctgccatcc tcggcgccct cggtgaggcc ggccggccgg ccgtcttccc ggtccacccc
     3061 cggacgagga agtacctcag cgaatacggc ctccttgtgg agatgccgga gaacatccgg
     3121 gttatcgagc cgctcggcta cctcgacatg ctccacctga tggcgcacgc agaaaagatc
     3181 ctcaccgact ccggcggcat ccagaaggag gcgtacatgc tcggcgtccc ctgcatcacc
     3241 ctccgggaga acaccgagtg ggtcgaaacg gtggaggcag ggtggaacgt gcttgtggga
     3301 gcggagaagg agaggattgc agatgccatt cactctttat cgcccatatc gcgacagaat
     3361 ggtatattcg gcgatggaga agccagcctc cggatccgga gaattctcac cgggcgtcaa
     3421 aacgcccggt ccggatgagg ggtttgcggc atgccccggc cggagatcct gtttctcacg
     3481 agcagccttt cttcttttgc agacggagac ctgcgcatgc tccggaagac gtacccggtc
     3541 agggaggtcg tcaccggggg cggcccggcc gggagcctga tgcagaggct ctccctggga
     3601 cttgagatcc tctccggggt tctcagaaca gacatggcat tctcnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn ccgggaatgg caaaaaaaat ccggtacatt atagagaacg ccgactgtat
     3721 ccttgcggtc tcggagttca gcaggaggga gatcctgcag gtcgccggac cccgccggat
     3781 cgagacggtc tacaacggta tcgatacttc ggtgttttcg cccggaggcc ggaaagagaa
     3841 tatcgtcctc acggtatgct ttgtcagcac ggaaaacatc aggctgaagg ggctcgacac
     3901 tttcatagat gcagcccggc atcttccaga agtaaggttt gttctcctcg gccgggccct
     3961 tgatgacgct ctcgaaactc tgaaacgggg tgctcccgat aacgtcgagt ttgtcggggc
     4021 tgtcgggcag ggtgaactta tcgaatggta tcgccgggca aaggtctatt gccagatctc
     4081 ctaccgggaa tcgttcggtg tcgccctcgc cgaagcgatg tcctgcgagt gtgttcccgt
     4141 ggtgacggac aggggcgccc tcccggaggt ggtgggcgat ctggggtttg tcatcccgta
     4201 tgccgatact cgggcgacgg tcgatgcaat atcgcgagcc ctcatctccg acgccggtcc
     4261 ggctgcacgg aagagggtct gcgatacgtt ctctgtagag cagagggagc aggagcttcg
     4321 ccggataatc gcgacactgc agtcgtgatc tggttcgttg ctgtcctgaa gtcaccctgg
     4381 aactatcttc cctgtctgcc cgcccgtctt ctgcccggag gttcctctcc gcaacaaagt
     4441 ataagtgtct ctccgataca gttgggtcca ttcagtcctc ttattcgtgc ccggggatgg
     4501 taatcggggg aaggctccgg gcagggaggc ctgagacccg actatgcgta tctgcatgct
     4561 caccacaacc cacctgcccc acgacgggcg aatattcgag aaagaggcga agagccttgc
     4621 aaaggagcac gatgtcacgc tcatcgcccc gtcggatgct ggaggcgtcg gagaggacgg
     4681 aggcgtgcgg gtcgtcaccg tcccaaaacc cgcatcaaac ctccttcacc cggtgacgct
     4741 ctggcggacg ctccgggcgt gcctgagcca ggagtgcgac gtcatccact gccatgagcc
     4801 ggatgcactc ctgatcgccc tcctggtgaa ggcagtgagt ggtcggaaag tcgtctacga
     4861 catccacgag cactggccga gcgagatccc gtttgacctg ggcctcccga aagcgactgt
     4921 cctcacggga atccttgagt ccctgctctc gccggttgag gtcgggcttg cccggtttgc
     4981 cgatgcgaag attgcagtca gcgagagcgt cgccgagcgg ttcaggggaa acgggagaga
     5041 acccgtgatc atatccaatt tttcggtcgc cggctcggtg ctgcctgctt cgcagatggg
     5101 aagcgggcac ggcgtaatgt acatggccgg aaacatgcag ttgttccacg gcatccggga
     5161 gtgcatcagt gcaatgtcga aggtgacggc gaagtatccc gatctttccc tgacgctcgt
     5221 cgggaacgtt cgtgaggatg tcggtgcgat cgcggcgagg acggatccga gacctgagat
     5281 cacgctgacc ggctacctcc cctaccggga gatgtacgag accctgtgca cggggagcgt
     5341 tggcctcctg gtatttcagc ccgattacta caacgcctat atcggcctcc cgaacaagct
     5401 ctttgactac atgctctgcg gcctccctgt cgttgcaagc gattttccgg agatccggaa
     5461 ggtggtcggc ggggcagagt gcggggtgct ggtcgacccg acggacccgg atgcgatcgc
     5521 ggaggcgatc gtctacctgc tggagaaccc ggacgaggcg cggaggatgg gggagaacgg
     5581 gcggagggcg gtcgaggaaa ggtataactg ggggaaggaa gaggtgagac tgctgagcct
     5641 gtaccggtcc ctgaacggtt cagggatggc ttgaagatcc ggtggggatc ggcgtaacta
     5701 cctcttctgt tgctctaccc ggttcgtggt attggtggat tgccgtatcg tagatgcgaa
     5761 ggatatctcc tgctatcctg tcccatgaaa acttctccgc gaattgcagg atctcctcac
     5821 ggttccagtc tttctccagc ccaatacaga tcttctctgc gagatcgtct tcgtctccgg
     5881 gctgacagaa cagcccgtgc ctgtcggaga cgatgatctc cctgcttccg ggagtgtttg
     5941 ttgcaatgac gggcttccca caggccatgg cttctatctg gacgatcccg aaactctcgt
     6001 tcagactcgg gagaacaaag aggtcgcatg cgttcatcca gagcggtatc tcgttgtgag
     6061 gtttgttccc tgccagcatc acatgttccg acaggccttc atcgacgatc tgcttctcca
     6121 gcgctgctct ctgtggtccg ttgccgacga tgacacagag caggtctgga tattcccgga
     6181 cgatatttcg gattgccggg atgagatggc ggtggccttt gatgacttcc aggttcccga
     6241 cggacagaat gatgcgtcgg ttttccggca gcccgagttc tgttctgcag gccctgctgt
     6301 ccatcggccg gaagattgtg gagaagccgt ttgaaatccg gaggaccttc tcgttgtagt
     6361 tttgtaatat gggtatatcg taggtgtttg ctctcagcac cgcatccgag ttcagccagg
     6421 ctttctggat cagggggtga ttcatattgt tctcttcaga cagccatgcc gggtcctcgt
     6481 ggatcgtcgt gatgaccggg atcttcaact tatccttcaa cattacaccg atgtaggccg
     6541 acggccacgt gaaatgagcg tggatcagat cgaatctctt tcccagcttc ctgagcaggc
     6601 gctccacgac gtaggggcgg gtgtcaaagg atagtttcga tctgttcgtg acccccggga
     6661 gagggaggca ccttgggagg aagaggcagc gcgggtagaa tactgagacg ttatcatact
     6721 ggtagtccct gcagagccgg tcgttcggca tgacccgtcc ggaaaagaga acgggtgcca
     6781 ccacgacgat ctcctggaaa tacggtttta tcgtatcgac aaagtttttt atgaagatgc
     6841 cgccgacata cgtatctgtc gaatcagggt aatcgggggt gataattaag agattgcgtt
     6901 tcataataat caggcaggag acc
//
LOCUS       DAJB01000022            3076 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_3912, whole genome shotgun sequence.
ACCESSION   DAJB01000022 DAJB01000000
VERSION     DAJB01000022.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_3912"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          676 a          968 c          891 g          541 t
ORIGIN      
        1 ggtggcggcc ccgggatctt caacggcaac cacgtcgtga ccagacacgc cgcaggcgtg
       61 gctatcccgt gtgtggtcgc tgcggcggca ctcgatgccg gaacccagat gttctcgccg
      121 gagagcactt ccaaggtcta cggcgacact ttcggccagg ttgacgtctt cagcaagccg
      181 atccagcaga tcgcacaggg tgtgtaaata acataaggat gctaatgact gacgccatat
      241 accctcaggt tcgaatcgtc tctgcgcgat ttctcagacc cgaaaccgtg gaacaactcc
      301 tgaacagact tgttcagata ggcgggattc gtcgtatgtc cctcaacgga cagaaccttc
      361 ccgccaccgt tccgtacggc ccggcacggg ggaaacccaa tccccacccg gaccgtaagg
      421 tcatccacat cggagaccag gacgtccagc tccgggtaca ggtcggaaca attatcctgg
      481 agcttgagga cgagtcgtcc attccggcaa taggagaagc ggttgatgaa gtctttgccg
      541 ataaggactt ctcctgcacc gtccagcagg ggcggtacat gaagacccag ccgacgatgt
      601 ccgacttcgc caaatacggg cctgacgccg acagagagat tctcgggctc gtcgatccga
      661 ggaggaaaga cggtcccgtt atcattcagg gaaccaagtg acatgcctat cggaagagtg
      721 acccaggtcg ttgattgccg cgagagcatg gggatgggaa aaggaggcgg tctcgcccag
      781 cgtgggacca tctctgaagc caggtccccc gatgtgatcg tgattggaat gtcccccggc
      841 cgcagacacg taacaaaacc ggtctgcgac atcacctccg gtctccggag agagggggca
      901 gagttctccg tgactacgct tgtcctcaat gcagggagcg gggttccggc agattctccc
      961 gtcgcgggtc acgttctcgg agcctacttc gggctgacgg agaaagagat cgttcagatt
     1021 gagcagcaca aggtcgccat cctgcaccac gggaatgtcc gctcccatgt cgtgcagaag
     1081 gtccggttta tccttgagca tgtgaatatc cgggcggtcg tcgtctctca ggtcccgatc
     1141 gacttcgaag atctcgcaaa agaggggatc aggacagcgg tggttatgcc gccgcccaac
     1201 cgggtcaaga ccaaaggcat ggtcatggat attgtcagtg gtgtgacacg aggtcagaca
     1261 cctggcaggg aaaagttggc ggcagtcatt cgtgccgtta tgaaagtgct aaaaagcaca
     1321 acatgaggtg aatgaaaaca tggcatacaa gccccaatac ggaccgggta catccaacgt
     1381 tgccgcaaac cggcgcaagc agatggaccc aagccagaag cttgaaaaga tgcgtgatgt
     1441 gacagatgag gatatcgtgc tgatcctcgg ccacagggcg ccgggagccg cctatccgac
     1501 ggcccacccg ccgctcgccg agcagcagga acccgactgc cccatccgga aaattgtaac
     1561 tccgactgac ggtgcaaagg ccggcgaccg cgtccgctac atccagttcg cggactcgat
     1621 gttcttcgcc cccagtcacc cctaccagcg gacctacacc gagtgctacc gcttccgcgg
     1681 tatcgacccc ggcacactct ccggtcgcca gatcgtcgag tgccgtgagc gtgacctcga
     1741 gaagtactcc aaggaactcg tcaacaccga gctcctcgac gcggcccgca ccggcatccg
     1801 cggcgcgacc gtgcacggtc actcgctccg tcttgccgag aacggcatga tgttcgatat
     1861 gctccagagg agtgtccttg gcgaagacgg catcgtccgc tacgtcaaga accagatcgg
     1921 cgagcccctc gaccgtgcgg ttgccatcgg caaaccgctc gacgagaagt ggctcaaggc
     1981 ccacacgacg atcttccact cgctcggtgg caccccgtac cgtgacgaca aggagtacat
     2041 cgagtacgtc cagcgcatcc acacgctgcg gacaaagtac ggattcatgc ccaaggagtg
     2101 atgaaaatgg caaagattga aagatcccag aagctgttcc tgaaggcact caaggagaag
     2161 ttccaggggc aggacgtcca gtccgagacg actgagttct acaaattcaa cggctaccac
     2221 cagtctcccc gcaaggagga gttcgtcaag gcgagccgtg ccgtcgagat ggaccgcggc
     2281 atctccatgt acgaccccgt gcgctgccac cttggcggta tcccgctcgg ccagcgccag
     2341 ctgatgacct acgaggtcag cggcaccggc gtcttcgtcg agggcgacga cctgcacttc
     2401 gtcaacaacg ctgcgatgca gcagatgtgg gatgatatca ggcggaccgt catcgtcaac
     2461 atggacctcg cccaccagac cctgcagaag cgtctcggta aggaagttac tcctgagacg
     2521 atcaacgagt acctccacgt gctgaaccac gccatgcccg gcgcggccgt cgttcaggaa
     2581 cacatggtcg agactcaccc cggcctcgtc gacgactgct acgtcaaggt cttcaccggc
     2641 gaccaggaac ttgccgacga cctcgaaccc cagttcgtcc tcgatgtcga gaagctcttc
     2701 cccggcaagc aggccgaggc gctctccgca gccgttggaa agtccctctg gcaggccatc
     2761 cacatcccga ccacggtcgt ccggacctgc gacggtggaa cgacctcccg gtggtctgcg
     2821 atgcagatcg gcatgtcctt catcggtgcc taccgtatgt gcgccggtga ggcagcagtc
     2881 gccgacctct cctacgctgc gaagcacgca ggcgtcatcc agatggcgtc ccacctgccc
     2941 gcccggcgtg cccgtggccc caacgagccc ggtggccttg cattcggtct cttctccgat
     3001 atcatccagg cgaaccggaa gtatccgaac gaccccgcga aggcgtccct tgaggtcgtc
     3061 ggtgccggaa ccatgc
//
LOCUS       DAJB01000023           24674 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_50477, whole genome shotgun sequence.
ACCESSION   DAJB01000023 DAJB01000000
VERSION     DAJB01000023.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 24674)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24674)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24674
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_50477"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         4339 a         7770 c         8127 g         4438 t
ORIGIN      
        1 atgctgatta taaaaaaatc tatgagctct taaacaaggc atggctgacc gagaatgacc
       61 tcggggtgta cgccgggacc tcgacggtgg ccgagtgcct ctctatcctc aagaagggaa
      121 acctcatcga agaacagtgg cggatgccca agcccggaga gaagccgacg aaggaatacc
      181 gggcgacata cagcaaattc agagcgagtt ttcagtgttc tatgggtgat atcggagatc
      241 tgctgcatat cgccgtctca aacgacgagg ccctccgtac gatcgtggac tcggtcgagc
      301 aggagattgt ggccgggacg acctcgatcg gcgacatctc ccggaaatac ggggtcagcc
      361 cgaccttcat caagggactg gcgaagagga ttcccggtct cgacgtgaaa gggcagggaa
      421 tggtgttgct tgaccgactt cactgacgac ccgctctacg tcatcctgcg cagtaaacgg
      481 gaggccaccc ggttccagat cctggtggag atcgccgaac accagccggc cgtccggcag
      541 caggagatcg ccgggaagct cggcgtgaca ccccaggccg tctccgagta catccgggaa
      601 ctggtggatg agggcctcgt caccgctcac ggccggggcc ggtacgaggt gacgaagaac
      661 gggatcgagt gggttctgcg gcatgccgag gcactcgaat cctatgcccg ccatatcaac
      721 cgcgacatca tccagcaggt ggcggtctgg acggctatcg cccgggacga aattcacaac
      781 ggggataccg taggcgttct catgcaggac ggctggctct atgcgacgaa agaagagcag
      841 agcgctacag gtctcgcgac catggatgcc cagcccgggg aagatctcgg tgtcgcccgg
      901 ttaaacggca tcatcaagca tgaggagggg ctcatccacg tctgcaaggt gccgcgggtg
      961 gagcggggcg ggtcccgaca ggtctcgaca gacctcctcc gggatgctgt ccgggacacc
     1021 gagatggtgg ccgccgtcgg cctcgagtcc tatgtggctc tccggaaggc ggatatcaag
     1081 ccggacatgt tcttcggctc ccgggagggg gtcatcgagg cggcgttcca cggccgggag
     1141 tgcgcaatac ttatcgttga tgaggagttc accgacttcc tcaagcgact ggagacggtg
     1201 gggctcacct acacgatcca cgacctgatc gcaccatgaa ggttatcgtc cagccccaga
     1261 aaggcacata taagttactg tttgtcgaag gcggccgcgt cgaaggctcc gggttcgtcg
     1321 acctgaccgc gactccgaag ggccagcggc ccaagaactt caagatgcgc cggcggggca
     1381 aacaactcaa gcccaccccg acccgtgacc tgatcacact gctccgccgg tcatcggtgc
     1441 atcttgcagc gacgagcccg gagtttgagt cgttccttgc cgacctccag atcccgtcga
     1501 cacggatcaa catctgccgg ttctgccagc tcgaggaccg gtttgtgccc gttgataaga
     1561 cgaccggtgt gcggtacggc ggcgaatgga tctgcatgga ctgcgctaag cgcgagatgc
     1621 gccgggaact cggctacctg gggaggttcg ggggctccgt gctcgtccac ctcgagaaac
     1681 tcctcgtcca ggtccgggac ctcgaccggg tgctggcgtc ggtggggccc gaacatcccg
     1741 accggaagcg aaccctcttt gaccggcttg aggcgcacga ggtccagaag acggaccaca
     1801 tcaccgacct cccgctgccc cccgcgtttg cgcaggctgc cggggtcgat tacctgatgc
     1861 ccgtccagca gcttgccgtc caggaagggc ttcttgaggg acggcacctc ctcgtcgtct
     1921 cggccaccgc gagcggcaag accttcatcg gggagatggc cgggatgaaa aacttccttg
     1981 aagggcgggg aagaatgctc ttcctggtcc cgctggtcgc actcgcgaac cagaagtacc
     2041 agcggttccg tgaccgctac ggccaccttg tccgggtcac cctgcaggtc ggggtgagcc
     2101 gcctcaacct ccctgagacc cgcccggcgg gggaccggga cgtcaacgct tccgtcgtgg
     2161 tggggaccta cgaggggatc gaccacgctc tccggaccgg gaggtcgttg aaggacatcg
     2221 ggaccgtcgt catcgacgag gtccagatgc tcgaggatcc cgagcggggc caccgcctcg
     2281 acgggctgat cgcccggctc aagcacatcg cccccgatgc ccagttcctc tatctctcgg
     2341 ccacgatcgg gggtcccggg ctgcttgcgg agaaactccg gacgaacctg gtccgctacg
     2401 cggatcgccc ggtaccgctc gagcggcacc ttatcttcac cgagcggtcc aagaagatcg
     2461 ggttcatcaa gcagatggtc atcgaggagt acaaagtgcg gtcctcgaag ggctaccggg
     2521 gccagacgat cgtcttcacc aattcccggg cccgctgcca cgtcatcgcc gatgccctcg
     2581 gcaagaatgc agctccctac catgcgggac tgaccgccca ggaacgccgg gacgtggagc
     2641 ggcggttcgc gaacggggag atcgccgccg tcgtgaccac ggcggcgctt gccgccggcg
     2701 tcgacttccc ggcatctcag gtgatcttcg acgcgcttgc catgggcatc aagtggcttt
     2761 ccgtccagga attctaccag atgatgggcc gggcgggccg gccggacttc cacgacctcg
     2821 gccgggtggt catcatggcg gagcccggcg gatcgtactc ccgggcgtcc gggaagaccg
     2881 aggaggaggt cgctgtcggt ctcctgcggg gcgagatgga ggaggtcgct cccgagtacg
     2941 acatggagca gagcacggag gagttcgtcg cgaacgccgt ggtctgtggc ggcgacgagc
     3001 agcaggtcat caagatgacc cacacgatgg tcggggcgct ggagccggcc ctcccgacgc
     3061 tccttgacta cgggctggtc cgccggcggg cggggcgtat cgaactcacc gatatggccc
     3121 gggtgatggc cgagcacttc atcggcgccg agcgcctgat cgagatcaag gacatggtcc
     3181 agcgggtgga cgatgcggcc gagatcatcg cagaactcga atgcgctgag gagaagacgc
     3241 cttagggacc tgggtcagca ccctttctcg tactctgcgg atacagcccg gatccctctc
     3301 cctcaccggt accgctccag gtactcccgc accttctccg actccaccca gccgtcgtcg
     3361 agctgccgga ggatcgcctt gatctgccgg acctgctcgc ggatcttctc ggcgcgctcg
     3421 tcgtcgatga ggtcggccat cccctggacg acctgaaggg ggtttcggat gtggtcggtc
     3481 aggaccgcga actgctcgat atttctctcg atctgccggt atgcttccct cctctcccct
     3541 tcaatcctct cccgctcttt catctcgcgc ctgagactct cgtttgccat ctggagttcg
     3601 ttggtccgtt gcgtcaccag ttcctccagg cggtaacggt agtccatcag ctccttctcg
     3661 gcccgccgcc tctccgtcac atcccgggca atgatgagga tcgccggcgt gcccccgagg
     3721 ctgatgcggt ggatgctcgc ctcgacgggg atccgctcgc cctcccgggg aatcaggtcg
     3781 gcgctgtaga actggtggtc gcctctcgcg aagtcgctcc tgatgaggat gatcgaccgg
     3841 ggagcgacgg cgaagagttc ctcccaggca tacccgaggc accggcaggc gagcgcgttt
     3901 gcgtccagta tccggtaggg cgtcccttcc ctcccgactg cgcatacgag caggaagtcg
     3961 ttcccgctgt caaagaggag gtggtagcgg tccctgctct cccggagttc ctgccgggag
     4021 cgttccacct cgtcgagcat caggttgacc tcgtcaacaa accgtgagac cggatcgccc
     4081 ccgattgcag ggaggcgggg cgggacactg ctccccgtct tgatggcctc gatgctcgca
     4141 tccatgccgg cgatgcgggc ggccacttcc cgctccttga gccagatgcc gagcagcccg
     4201 aaggcggtgc cgatgaagag gagttcgaag agcaggacca ccggggaggt ccagattccc
     4261 cccgggagat cggacgggag ggcgagcgcg aggaggaggg cgggaccgcg ggcaccgagc
     4321 gtgcaggagc ccgcaggtgg ggcagccggg aaccggggtg cattcttgca gaggtccatg
     4381 ctgctgtatt atgtcacttg tgccgggtgg ctacccggcc gtggcctgcg ggatgtgtac
     4441 ccgggaggtc ttccggttgc cctgcaggtg gttatcatgg ctattccggc gcgctgatgg
     4501 aaagcgcacc actgtaaccc aagatagtaa attgagtatt taatattttc ttatattgga
     4561 gtagagttta cggtctgaat agctcttttc aatccgaagt gcgcacgttt gggggtatca
     4621 gaaggggaat atcggtcttt cccgcggcgg caccgggggg tgtgaggggc aggtggccta
     4681 cccggactcc ggaagatctc cgccggcact ttgccgggtg ttcctgcgca ttagcacggc
     4741 gaagatcccg gtcccgatga ggcttatcgc cccgcccacg tagaagacgg cgtcgacgcc
     4801 gatagaatcc atgaagaccc cacccatcag cggtgcgatg atcatgccga ttccgaatat
     4861 cgtattgtaa gcgcccatcg aggttcccat cccgatggtc cggcccgcat cgactgtcag
     4921 ggccatgatg gccggctgct ggatggcacc tccgacgccg atgatggagg tgatcgcgag
     4981 caggggccag aacgatcccg agaaggggat tgcggcgagt gctaccgctg ctatgcctga
     5041 gccgatgacg atcagggcga ccttgttccc ggtgtcggtg agcctcccga tcggcacctg
     5101 gaggagcgcc atcaggaagg tgttcgcgga gaggatgatg cccacttcga atggactgat
     5161 tgctatcagc gggccgaaga ccgggatgaa gaccatcata cctccgcggc cgagagcact
     5221 gatgagggtg aagatcatga cccctcgcat gaccggcagc gtgaagatcg cccgcatcgg
     5281 gatgggtttt gcatccggga ccgtgaatga gcctggcttc gcctcgggga gggagatggc
     5341 gacgaggagc gtggcgaacg ccgagaggcc ggccatgaca taaaagaccg agtccatccc
     5401 gaacgtgtcg ttcaggaacc ctccaagcag cggccccacc cccatgccga ggaacatcga
     5461 gatggagaaa tttcccatcc ggctcccttc ctttcccttc tccgagaggt ccgcgatgta
     5521 ggccatggct atcgggacga ccatggctga cgcgatcccg tggatgaggc ggacgaccgt
     5581 gagggagtag acgctgtcgg cgatgaggta ggtgagcgac gtgaccgcgt aggcgaacat
     5641 gcctataagg atgatccatt tcctcccctg ccggtccgat atccgcccga tgacgggcat
     5701 gaagaccgac cgggagagcg cgaaggccga gaagatgatg ccgagccaga tgccggtcgc
     5761 tccgagattc tccgcgtaga tggggaggag cgggctgacg ataccgagcc cgagcatggt
     5821 agcaaagacg gagatgaata agacgttata gattcgtttg gcagtattca tactggttac
     5881 accgggtttg aggaccaccg ccgcagacag acttgcggcg agcacgttca caattgcgag
     5941 aaaaaaagtt ggcgatgctc tgccttatat attggctcgc cggccgggat cacatccgcc
     6001 gcgcgacgca gcaccaccgg tcgtagatgg ggtcgcgctg gttctcgaag acctcggcgc
     6061 tccgcccctc ttccttgcac cacccctcca cccgggccgc ctcggcctcc gtgccgacgg
     6121 tgatcagcgt ctcatcggtg agggcgagga gcccggtgat gatcggctcc cagaggccgg
     6181 tattgtagga gtagatctcc ccggccatga acgcggcgcc gaggggcacc ggagtaacgt
     6241 aacaggaagc ctcctgcgcg tcgatgcaca tggtatcgga aggcgcgagg cggccccgga
     6301 tgagcccgag tgcgaggagg gccggatcgt tgtcgtaggc gagcgcccgc accccgcccg
     6361 cccggagggc cgcggtgccg acacccgacc cgcagcagca gtcgaggcag ggggttcccg
     6421 cgcggtcgcc ccagacttcg tcgaggaggt cgcggatcat ctccgtccgg tcgggacgca
     6481 ggtcttcgag ggcagggtgg acatttctgc agatatcgag gctatagtat tcacggaccg
     6541 ccgagagcca cgcatctctg ctctcctggt agatctcccc gccgacggtc tcgaagcatt
     6601 cgatggcggc gagcgtcgga tcccgaaaga ggaacgaggc ggcatgccag ccctcgtcgt
     6661 cgtggagcgc gagcgcgagc ggctcctcct cctcgtcgac caggagacgg ccttcgtccg
     6721 gcacggcctg gaacagcagg gccgtacagg cgtctatcgt cagttccgca aacgacggtt
     6781 caaccagctg gcacccgtcg acctcaagga tatctgtaac cttcatgtcg ctcgcctctc
     6841 gatctttaag ccccccggtg cccggatgac cccgtccacg cgggcgtcct cgtgcagggt
     6901 gagacactgg caggagacgt tcccgaggat atgcacctcc tgctcgacca ccacgtcgcc
     6961 gtcgctctgg acgtcgccgt ggatgaccga tcccctggcg atgctcgcac cctggcgcag
     7021 gtggaggctc ccgaagatcg tcgtctcctc ccggaccgca attgagctcg cacggatgtt
     7081 cccgtgcagc cgacaccctg ccccgacgac cattctctcc gggaccgaga aggtctgcat
     7141 attgaggatg gcgccggtcg ggatcatcag cggcgtggca gcactggcct cttcatcgcc
     7201 gaagagtttc tggaggacag aggtcagttc ctcctccttc tcgatcccga ggatggccac
     7261 caggtacatc ataatgtaaa tgatgaccgg catcggattc cttatggaga tccagccttt
     7321 cgcctcgaac ccgcgctcga tctggacgtt gtccccgata tcgagatcgc ctctgacgat
     7381 cagcttcccc tggatcttca ccccctcgcc gaggtaggcg tcctcttcca cgagaacatc
     7441 gccgttgatc tcgcaccagt tatctattct ggcgtcgccg cccgcaacga tgttaccgtt
     7501 gatcttgctg aattcacaga ccacgacgtc gttgcccgaa agaccgtaat cgatccgaca
     7561 cctctcaccg atgacgatgt cctgctcggt cttgagtgtc ctctcctgga gctcggtatt
     7621 gtcggggagg gtgcaggccc tgatccagtc gtggtctcct gtcggttcca tttacctgat
     7681 atagtcttca tgcgccatac ttatgactct tattaattag atccagggca atcggcatga
     7741 gatcgcagcc gcggatacgg tggagcccgc cctccgcaca cgagacctcg tcgaaccggg
     7801 tcgtccggtc ggtccggaca cccgggcccc tgatgacgac cgggaccggg tcggcgctgt
     7861 gatctttgat cgaacagggc gtgctgtggt cggcgcagac gacgagcagg gtatcggaga
     7921 gcgtgagcag cggctcgagc gccgcgtcga tcacctcgat gaagtcccgt ttctggatgc
     7981 ctttcccatc atggccggcc tcgtccgcgc ccttgatgtt catcagcacg aagtctttcc
     8041 ggccgagttc ccctatcgcc gccttcacct tggcgttcag atcggagtcg accgacccgg
     8101 tcgtccccgg caccgggatg tgctcgagcc ctacaacctt tccgatcccg gagatgaggg
     8161 tcgccgcgga gatgacgctc ccggagagtt cataacgctc ggagaacggc gggaacgcgc
     8221 ccatcttccc ggcaccccgg atcaggagga agtttgcggg tgggaccccc tcttccatcc
     8281 accggagatt tagcgggtgc tcgaagagaa tcttcgagga ctgacggatg aactcgttgc
     8341 aggcaacggc tgtctttaca tccgctgcgt catcggtgca ggaccggatg gtgagcggtc
     8401 ttaccccttc cttcttcggg tcgttcgagg agaccttatc cccgagcccc tccccggtga
     8461 gggcgagggc cgcccggtgg ccggcgcccg actcgagcct gaatccgaga ccgagcggcg
     8521 agaggtccac accgtcccgg atagcctccg agagcggttg ggtccgggag atccgcccgg
     8581 cccggcggtc ggtgacgagg ccgtccgcgt ccacggtggc aaagttgcac cgaaacccga
     8641 tcatcccggc ggtcatgctg atgccggtcc cctcggcctc cagcgggccc cggccggtgt
     8701 agtactcctg cggcgggtag ccgagcagag caaggtggga ggtgtcggac ccggggcgta
     8761 tgccgggcgc gatggtatcc atgatcccgc atacgccctc gcccgcgatc cggtcgagaa
     8821 ccggggtccg tgcggccgca aggggggtca gtccatccag cgcttcacag ggacggtcgg
     8881 agatcccatc cagtaccagg aataacacct tgtgagcgat catcgagaac agatgagatt
     8941 ttggacctga ttactcttcc cggtggatgg cgatgacccg ggttgtcagg ccgctccctc
     9001 ccttctccac gagtgccagg caccgctccg catggtggag cgcctccttc gcgagggctt
     9061 cgctgtgctc gcggcattcc tctccgcagc cgatcatgac gaggacgttg gccgtcttga
     9121 tcatatcggc tgagaggcac cggatctcca cggcgatgtc ttcgccgagg atcgcctcca
     9181 catcgcatat gcagtcgtag agctggtgtg aataccggtc gagcagcgca tttgtggcga
     9241 gaacccgtgc ggcgccgctc tctctctgtg caaggtcggt ccactcccgg tggaactgaa
     9301 cgagcatcct cctgatgatc ccccgggcta tcgtctgctg ttcgtcgaaa gcctgctcgt
     9361 tgagcagaac ccggcctggc cgccgcgtac ctgcatgctg ctcctctgcc gtcaccatct
     9421 tcacctcttt gccacgctcc ggcggaggtg tatcaggtca tagggcatat acccttgcta
     9481 tctccgtcac cgggacgaga gaacggtgaa agtacaaatg gtaataaaat tggggtgagc
     9541 agccggcctt atacggccgc gacctgctcc agtttggact tggtgatctc gacccggcgg
     9601 atcgggtaga tcgtctttac ggccttgaag acgtcccggg ccatgtcgcc ggagacgatc
     9661 tctttgacca gggtctcgaa gtcgctctcc gccgcgcggg cggagagagc ctgcgagatc
     9721 gcctgccgta cggcgtggac ctggctctga tcggcccttg ataaggtgag acagacgacc
     9781 gtcacccgca ggagtttctt gtccttgctg gtgatcgggt ggatggtgtc gatgcgggat
     9841 gcccgccgct tgaccatcga ccggaggtag tcccgggtca cttcgtgacc gacgaactcc
     9901 gtgtacgcag cgtcgccggc cacgttgttg atcttaaacc tcatcttgat gtgggacttt
     9961 gagtagtcct gcacaacttc accgagcgtc gcggtcatga tgcggccgac catgttcgcg
    10021 gggtcggccg agatggcgtc gccgatctca gctttgccga aggcatcggg tacgtagaca
    10081 cggtaccact tcttggcctt ccagccttcc agccttcttc caacctgttt cttccttgcc
    10141 atggtatcac ttctctctgt tatatcaatt tttcagccca aaggagcctg aacacactct
    10201 ggaactctcc gctctgccgt caccgtgagg cggaggtaca tacgtcctct gctatcgtca
    10261 ggttcatgag gtaatcgtcc accgaagcga tgattgaccg gagtctggtc ccgtcgatct
    10321 cggcccgcac ccctccctct atcgggtagg tcctgatcag ggtgaggtta tcgggctcaa
    10381 gtgccgccgc cacgcattcc ggatggctgt gcggcgtctc gatggtgccc tcgatccgga
    10441 tcatgcgggc accgcctcca ggagttcctg cctgaaccgg tccacctggt cggcgtcgat
    10501 ccgggcgccg gccctccggc gatgtccgcc gccctggccc ccgcacgtct ctgcgagtct
    10561 ccgcatcagt gcatcgaggt cgaggtcgat gcccggcggg cagcgtgccg atacggagta
    10621 acggccccca tgctctccga tgacgaagac agggccgctc tggaccaggt cgtttgcgag
    10681 cgcatccgcg acatcgcttg ctacccctcc gtcgtccacc gcaaagaggg cgacgcgctc
    10741 gtcgaggcgg cgtgccccgc ggatgccggc gatgaccgcc tgccggtagc gggcggtgat
    10801 ctcccaggct tccgggagag cgctggtcga gcggaggcag agcgatgcgc cgatatctcc
    10861 gctcccggcc ttgccgcagg cgtcgacgac ggcggcgaga ctcaaggcct cgtcgatcgc
    10921 ttcccgcccg aggctgtagg tggtccccca gatcccgcag agtgcgggga gggacgcttt
    10981 cggggcggcc gcaaggacga cccgggagag gagggactcg atatccagat cgtcctcgtc
    11041 ggtgacctgc gcaaccagtg cccgggctgc gtcgggatcg cccgagaacc cggtgaggta
    11101 gggattgacg gcgagcgcga gctgctcgac gaggcccctc cccgggagga ggaggccgcg
    11161 gcggggtgtg atgaacccgt tggcgatgcc ttcgctggat atgtcccggt tcggcccctc
    11221 gatatcctgg ccgtcgccga tgatcccgag gagcgcgagc cccgcgaggt cgcggttgtc
    11281 ccccatgcgc tgggcgacga tgtaggccgc gccggcagcc gagaggttcc gttcgccgtc
    11341 aatcccggcg aggtgtgggt tgacgtggta gtcgccctcg aagtggggca ggtggtgatc
    11401 caccaccatt acgtcgtcgg ggaggtccga gagtgctgat ccgaagtcgc agagaagcac
    11461 gctgccttcg cgaggcaggt cggccgtgga gatgcctgag cggatcctca gccggaaccg
    11521 tccgcccttg cggaacatgg cgtggcagag gatggatgcg gcggctatac cgtccgcatc
    11581 gtggtgcgcc agcacctcca caaactcctg ctcgagcagg cgaccggcca ggcgagcagc
    11641 agcggcgtca agcgacatgg atcatcagcg ggtgagcagg atctccgcag tctccggctt
    11701 gtaagtccag cctttcggca tccgtccgga cctgacgtag tacttgacca gtctgcgcac
    11761 cttggactcg gctatctgaa gctgccgggt gttgtgcaca tccttcttgt tctccgcaag
    11821 gtgtttcctt atcccgagcg ccttctgcat caggttccgg agatcttcgg ggatctcgga
    11881 ttcaaggccc ttctcgcgga ggatctcgtt tacgcgcttg ccagtggcga gcttgacgtc
    11941 agggacagcg taactgtccc gcaacgcaag gccaatctga ctggtcgaca tgccgtcttt
    12001 gcggagatca acgataatct tctcgatctc cggtgcgtcc gtgttggacc acccgggtgc
    12061 ttccttccgg taaggtcgga cagaaccgct ggttccacgg cgccgagcgt acattcttgc
    12121 cattgcatac acctccgtta gatatactat gttaagtcca gaaaagagtc cgttatctga
    12181 ctaatctctg taatcccgag cccttgcggg ccgtgctccg ggagcacgtc ggacttacca
    12241 tagccagcac atatggtgct gcatcatatt caccgggagg cgtcttaagg gtatcgggcg
    12301 ggtgtgctgc ggcacctgtg gcaggattga tccgtggagt ttattgtggt ttagaacgaa
    12361 tcctattgca tgaaggaagg gcattcggac agccagcccc acgatgcatt caccaggaag
    12421 cgccttctct cctggggcat cgttgtctcg gccctcctcg cgcttggtgc catggggtac
    12481 tccctctccg taggggtatc cgtcgtcacc tcccaccttt tctacgtccc gattgttctt
    12541 gcagcctacc gctaccctga ccggggcgtt gcctttgccg gtgcgcttgc cgccggctac
    12601 ctcgcggagg tgctcctcct cgcaccgggg ggcgggcttg aggtcgtaaa cgccctgctc
    12661 cgcgccgggg ttttcgtcgt cgtcgccgcc gtcgtatctc acctatccgg cagactgcag
    12721 gtgcgggaga gccgctaccg gggagtcttc gagacctcgg gggcggggat ctgtctcttc
    12781 tcgcctgaga ccgggaagat cgaggagatg aaccagaggt gctctgcgat actcgggtat
    12841 cccgacggtg aggtcccgtc cctggatgta tccgcgatct ggcccgggta ttccgggctc
    12901 gccggaaccc tcggggagag ggggatccag agcctggact gcaacctcgt cggccgggaa
    12961 gggaagccct gtccggtcct cctctcggcg aaccttcttc ccggtcggca ggagggctgc
    13021 gtggtggtca ccgggacggc agaacagaaa cagatggaga tggatctccg ccggttggag
    13081 gagacgttcc gggtcatcct caacaccact gatgtcggga tcctcctcac cgctccgggc
    13141 ctgcaggtcg tggaggcgaa tacggccgcg atccggctct tcggcggggc cgggccggag
    13201 gacctgatcg ggcagaatcc gggtgacctg atcgcagaga gtgatcggaa ggtcatccgg
    13261 gattaccggg aacgggtcct gcacggggag agtcctgccc cgcgagagtg catgttccgc
    13321 agactcgacg gtgtcgaatg gcctgcggag gtatcgctta cccgcctgaa gcggaacggt
    13381 gatgccccgg aacggctggt cgtctccctc cgggatatca ctgaacggcg gcaagcggaa
    13441 gaggcgatgc gtgaggagta ccgctatctc atggtcgtca acgagatcat cgccgctgca
    13501 accgcgtccc gggggctgga cgaccttctt caggtctcgc tcgcaaagac cgtcgccctg
    13561 cttgagtttg acctcggggc ggtctacctg atgcgttccg ggagcgacac ggcggtcctc
    13621 cgtgcccggg aaggggtggg cgaggccctg ccgccggtgg tgcgccggga ggaccccctc
    13681 taccaggaca ttctccaggc cggcgcgatg cagtgcatcg aggacttctc aatgaggtat
    13741 cccggctccg ggatccgggt tcttgccatg gtgcccatcc ccggcgatga cgggccggtg
    13801 ggatggatcg cggtcgggag cagggtccgg aagacgatcc cggagtccga gcgggggatc
    13861 cttctcggca tcgccgaaga gctccgcaac gcggtagtga aggggatgct ccaggaggat
    13921 ctggaggcgg cgctcgcatc cgccaaccgc tacctcgagg aggcgactgc ggcggccgct
    13981 gaggtcaacc tctacgtgga tatcctcacc catgacatca acaatgcaaa caccgtagca
    14041 atgggctacc tgcagatgta cctcgagtcc ccgaccggtt cggacggggt actcgtcggg
    14101 aagtcgctta cggccgtcta ccagagcagc gagatcatac ggaacgtcct gacgcttcgc
    14161 aggctcaaga ccgggcctgc cgagcttcgg ccggtgcggc ttgagccggt gatccggggg
    14221 atctgcagtt accacgcgga tgcccgcatc gcctgcaacg gagcggatac gacggttctc
    14281 gccgacgacc tcgtcagcga ggtctttgcg aacctgatcg gcaatgccat caagttcggc
    14341 gggccggggg tcgaggtctc catcagcgtc cgtgaggagg aggatacggt ctcggtgacg
    14401 gttgccgaca acggccccgg gatccccgac gacctcaagc cgcgcgtgtt cgaacgcaac
    14461 cagcgtggga caacgaagaa gagcgggaag ggccttggtc tttacatcgt ccggatgctt
    14521 gtggagcgct acacggggag tgtccgggtg ggcgatcgga tccccggcca cccgggggag
    14581 ggggccgcga tcacgttcac cctgccgcgt tgccttccgg caacggcgtg agccgaatat
    14641 ttatctcctc cctgaccaat atgtaatggt accagcgcga tagtagtcta gcggtaggac
    14701 aggggcttcc caagcctcca gcccgggttc gatccccggc tatcgcatcc cgattttccg
    14761 gctcccgaac gtgttagcac gttttgccgt tctgaagtct ccggtccgta cggcaggatc
    14821 cggttttgca cgcaggggac gttcgggccg gtccgcggtg cagtcccggc cctgcaatgg
    14881 ggcgccccct cacgcccgct gcggcccttc agcctcgtga tggagatccg cccgctgctc
    14941 cgcgccgcgg agtgcggagg cgatcaccgc cggcacggtc aggagcgtgg agacggcgaa
    15001 ggcgaaggtg atgccggcgg tcacggcctc ctgaacgggg atcggcatat tcgtaccacc
    15061 gatcacgaac atctccatga tctggtccat cgagaccgaa ccgatcagca cgggcatcgc
    15121 gatcgccatc gagatcacca tgccgaggtt gttgagcagg gcccggaccg aggaggtgat
    15181 gccccggcgt tcgggcggga cggcggccat gaccgcgctc gtgttcggcg acgcgaagag
    15241 gcccgatccc atgccgttga cgaacatcga gagcgcgatc cacgtatacg gcgtgtcgta
    15301 ccggatcacg gcgagcccgg cgaggccgac gagcgagatg acaagcccga tcgtgctgat
    15361 cgaccgcgaa ccgtaccggt ccgagaggat gcctcctacc gggcccatga tcacgagccc
    15421 gacggcgagc ggggcgatca ggatgctggc cgtcaggggg tcgagacccc ggaccccctg
    15481 gaagtagagg atcagcagga tcatcacggc gccccgggcg atcgcgttca gaaagaccga
    15541 gagttgtccg aatgcgacga tctggatgcg gaagagggag aggtcgatca gagggttcgg
    15601 aatgctgttc tcccgccgga cgaagaggac gagactgacc aggaagatgc cgagggtggc
    15661 gagcatcacc ggtgagagga agcccggcac gaccccgacg ccgacgtaga cgaggagcgt
    15721 catcacggcg atcgtgaaga gggccaggcc tgcatagtcg aacctctcgg cgccgaggcg
    15781 ggggagggtc ggctcccgga ggtaccggac gccgaggtag agggccgcga gcccgatcgg
    15841 gatgttgaag tagaagttca gccgccagtc gatcagggtc aggacgccgc cgaggaccgg
    15901 cccgaccacg gaggccgcgg ccaggatcat cgagaggatc ccgtttgccc gtccgagttc
    15961 tccccggggg aaggcgtcgg tgacgatgat cgtcgagttc gcgatcagga tcgaaccccc
    16021 cacggcctgg aatacacgga acccggtcaa ctggacgatg tcggtcgaga tgccgcagag
    16081 gagcgagccg agagtgaaga ggacgaggcc ggcgaggtag aggcgcttgc gcccgtagag
    16141 gtcggcggcc cgtccccaca gcggggccgt gatcgtggtg accagcatgt atacgatgat
    16201 cggggccatc acgccgatca gggtcgtgtt cagggcgatc atgatcgtcg gcagggcgat
    16261 gagcagcgtg gtcccattca gcacgccgcc gagagtgccg agcgaggcga tcaggagggc
    16321 gagccacttg taggtgaggg tcatgcgtcc ctcccgcacc tgcacgaagc cggcagttcg
    16381 gagcgctgga tgttctccac catgcggtcg agaatcgata tgagcgttgc ctgctcttcg
    16441 ttcgagagcc cggcgaggag cgcgtcgttg aggttgcgga ccacctcgtc gatgaggggg
    16501 gccgtcgccc ggccgcgttc cgtcagggtg accaggtagg cacgacggtc ggcggggtcg
    16561 cgttcacgga ccgcgtatcc cgcctcgatc aggtcgcgga tcgtccgggt agcgaccgcc
    16621 cggtcgacca tcaggtcctt tgcgatctgc tcctggtgga ccctgttgtg ccggagaaga
    16681 taactcagaa accggaactg gcgtgacgtt agaccgtcta cttcgaggcg ccgtgcaaga
    16741 taccggtgga cgaaccagtg aatccatccc atccggacaa caaccgattc ggaagacacc
    16801 ttagttgata tgtcaacaaa atatttcaag cagtcgacgg ctcgtcgtcc ggccggcgag
    16861 cgtagatggg gaggatcaag gggcgtcgac cggtacggtg cccgggttga ccaaatcagg
    16921 cgccgttacg gccgtcttct gcaacaatct cgaaccggca gtggtcgaac cccatcgagt
    16981 agcatcgggt ctcgaccgcc gccgccggcc ggccgtagtg gcgggagaag agcgcccgga
    17041 ggatgccgga gtcgaacgca catgcgggtt tcccggtcac gggcaggtcg gcgcactcga
    17101 agcagtcgta gacgaggagc acgagcggtt gcgcctccgc gatctcaagc cttcccaggc
    17161 ggtgctcctc ccagaatcgg gcgatattcg cgcagaaggc tccggtatcc gggtcggcca
    17221 ccgccgggta gagttcttgg ccgatccgct caccggcccg ggaaaggagc gggtcgagga
    17281 ggatcccctc ctgcatgagc gagacccgga tcgtccggaa cgcgagccgg tagaacgcgt
    17341 tttggtcgcc cgaccccgcc tggtacgccc ctgcataggc ggcgagcgca tcggccaccc
    17401 ggtcctccgg cgagacgctc gcgacgagat cgccgctgac gaagaatatc tttttgcggg
    17461 agtcctccgg gtcggcccgt gacccgatca cgcccgccgc cgccatctcc tggaggtgca
    17521 ccgagaccgt cgacttcgcc cgcccggcga gggcgacgat ctcgtcgaac gtacgttcgc
    17581 gctcccggag ggccgcgagg atagcacgcc gcaccggatt ctcgaccgca cgcgcccccg
    17641 tgggggtcga gtacaggtct atctccaggt ttgctttcgg ccgcccgggt ctcccccccc
    17701 tgctcacctc gtattccata gagtaaaatc gaaaggatga tatatcaatt gttcggatat
    17761 actggaacgt tcgtattgtt ccgaacaacc atggtgatag aaatgatggc tacagaactt
    17821 gcccccggcg tccactgggt cggggcgatc gactggaacc tgagggagta ccacggctac
    17881 accctcccgg gaacgaccta caacgcctac ctcgtccggg gcgagaagac cgcgctcatc
    17941 gacggcgctt acgccgggtt cgagggcgaa gtcctcggcc gtgtcgggtc cgtctgcgac
    18001 cccggagaga tcgacttcct cgtcgtgaac cacattgaga tggaccactc cggcaccctg
    18061 cccgcgttcg tgcggcggat gcccgatatc ccgatctact gcacggagcg ggcccgcgcc
    18121 gggcttgccc gccactacga cactacaggc tggaacatcc gggtcgttaa gaccggcgat
    18181 acccttgacc tggggggaaa gacccttact ttcctcgagg cgccgatgct ccactggccc
    18241 gactcgatgt tcacctatct cgccgaggac gccatcctct ttccgaacga cgccttcggg
    18301 cagcacgtcg caagcgccgc acggttcgac gatcagctcg gcagggatgc ggcgatggcc
    18361 cacgcgaaga agttctttgc gaacctgatc gtaccgctcg cgccgaaggt cttaaagaag
    18421 ctcgacgagg tcgggagcct cgggatcgac attaagatga ttgcgccgag ccacggcgtc
    18481 atctggcgct cgcatgccgg cgatatcctc ggggcctacg ctgactggag ccggggcgtc
    18541 gcaaaagaca aggtcacgat cgtctacgac accatgcacg gctcgaccgc gatgatggcg
    18601 aaggcgatcg ccgaaggcgt catggccgga ggcgccgatg tccgggtctg cctcctccgg
    18661 gacgggcggt acgaggggac gcaccgtagc gacgtcgtca cggaggtgct cgactcaaag
    18721 gcggtcctcg tcgggtcgcc gacgcttcag gacgaggttc ttcccacggt tgccgggttc
    18781 ctcagctacc tccgtgggct tgcgcccggc cggttagagg cgaagaagat cgggtgcgca
    18841 ttcgggtcgc atggtggtct gggcggggcg ttcgcccggg ccgaggaggc gctgaaggct
    18901 gccgggatcg aggtgatcga cggcggtttc caggtgaact accgcccgga tgacgacgag
    18961 ctcgcgcggg cttacgagct cgggcggaag gtggcacagg agatccgcgc ccggtgacac
    19021 ctccccggaa accggactgc acgtccggga cccgggatga caattactgc ctctttttag
    19081 ccgggtcacg ccggtgcggc aaacccgtgc ctcacccggg cgaacgccac gagcgccccg
    19141 acctgcatcg tcaccaggaa cccgacgacg agcgggacgg cgacggtgta ggcccagccg
    19201 atgaaggcac ttccggcgaa ccaggccgcc ccgtaaaccg tattgaatat accgtatgcc
    19261 gtcccgcgct tgctgatatg ggtgtagtcc gcgatggcgg cgcggaggat cgtctcctgc
    19321 gcacccatcg ccgcgcccca gagcaccacg gcgacgagtg cgagggcgta actcgtcccg
    19381 aaggcgagga aggggatgcc gatggcaagc agcggcaccg agacgagggt gacgagcccg
    19441 aacctgtcat atgcccggcc gatcacgagg gccgcgacgg cgtcgacgcc catggcgacg
    19501 gcgtagaaga gcgggatctc tgcttcggag accgtgccgg tgatggcgaa gtggtaggcg
    19561 atgaccggga acgccgcgaa cccggccatg gtgagcgcgg tgaagacgcc gtaggggagc
    19621 agggagcccg ggagggtggt tgcccgggag gcggggccat cgccctcagg cgacgccggt
    19681 tcgggcatcc ggtgccgggc gaagaagagg gcgaggatca tcagcgcgaa cgggatggcg
    19741 aggagggcaa acccgttccg gtagtccccc tgcgcaagaa agatgacgga aaagatcagt
    19801 gggccgatga tcgccccgat ctggtcgagg gcctcgtgca cggcaaaccc ccagccccgc
    19861 ccgaccgtcg tcgtggcgtc cgcgaggatg gcgtcccgtg ccggcaccct gatgcccttt
    19921 cccacccgtt cgaggacgag gagggccgcg gcagcctccc aggacccggc gagcgcgagg
    19981 agcgggaccg cggcgagcat cccgtagccg atgaacgtga ttgcccagta gtgccgcgtg
    20041 gtgtcggcca ggtacccgga gaccaggcgg agggcgtagc cgaggaactc gccgaggcca
    20101 gcgaccacgc cgacggcgag ggcgccggcg ccgaggaggt agaggtacgg tccggagacg
    20161 ctccgcgccc cttcgtagat gatgtcgccg aaaaggctga cgatgccgaa gaggacgatc
    20221 acggtgtagg ccgcccggtg tttgccgtcc atggaaggac ggtctgtcga tcatcatcca
    20281 taagagtctc gatcgggccg tgccccgatc cccgccggcc tgatggaacg ggaaggcgtt
    20341 cgaaccccgc gccgggggaa gatgtaatcg tgatgagcgg tatctacccc cgtggtgcgg
    20401 cacaaaacga gcattccgtg gggagagaga cggccgtgag gagccggagc tggatcgcgc
    20461 tctcgctctt catcagcggc gcggctctcg tggggcttct cgcggtgacc ttcacccccg
    20521 agacacgggc ttacctcggg gaggtcagca tcgccgccct cctcctcgct ctcgggttcc
    20581 gggccgcatc gatcctctgc cgggtagcgc ggatcggggt cctttgccgt ggtctcggct
    20641 acaccgtgcc gttccggagc ctcacaacca cccagttcct ctcgctcttt gccgggtcga
    20701 tcacccccgg gcaggtgggc ggcgagccgg ttcgggtcca ccggctctcg cgatccgggc
    20761 tcgccgtcgg ggacgccgtc gtcgtcgtcg tcgtggagcg ggtgctcgac ctcgcggtct
    20821 tcgtctccct caccctcgca gcgcttcttg ctctccggca cctctggggc taccttgccg
    20881 caaccgttct ctacccggtg gcggccttcc tcctcctcgt cctcgtcctc ctcctcgcgc
    20941 tcgtcgtcct ggtccgccac ccggccttta tgaagcaggt gataggcggt gttgccgtcc
    21001 gggtgctgaa ccggtgcggc cggagccggc gctggctccg gttctgcccg ggtgcggagc
    21061 atgcagggtt cctgggaacg aggatcgacc acgaggtcga gacctttgct gcgggttcct
    21121 cccgctttgt gagggccggc cgccgggccg tcgtcggcgc actcgcgctc accgtaatcg
    21181 aatgggtctt cctcttctcg acggcatcgg cgttgctcgt cgctctgcgg ctgccgccgt
    21241 ccctcccgga gtcgttcctc ttccagggaa tcctccagat gatcgcaacc atcccgctca
    21301 tcccgggcgc ggccgggata tcggagatcg gcgccgccac gctctacagc cggatcgtgc
    21361 ctacctacct cctcggcctc tttgtcgtcc tctggcggct gatcctctac tacctcaaca
    21421 tccccctcgg cctcctcgcg gccactcttg ccgcgaggga gaaggctggt agggatcccg
    21481 ctggcggtgg ggatcccacc gaaaacctat aacccttatg agaccaaaat ctatcaatta
    21541 acgcggatat gaccaaaaat gccctgttcg tgaaacgatc ggagagcatt gggatctccg
    21601 gcgggggaag atcgtgacca ccgataccga tatgtatgcc ctgagcatca tcaccgagaa
    21661 cagggcaggc gtgctgcgcg atgtagccac ggtgatggca gattaccagg ccaacatcca
    21721 gatgatccag cagtcgatcc tcacctccgg cccgaacacc gggaaggcct acctctactt
    21781 cgagttcgag gagtgcggga accacccgga gctcattgcc gacttatcaa gggtgccgtc
    21841 cgtcgtcgac gtcaccatct acccgccgtt ctcccggata ttcggatgca gggtgatcat
    21901 catcggtggg ggtgcgcagg tggcgcaggt ggcgctcggc gccgtgaacg aggcggatcg
    21961 ccacaacatc cgcggcgagc ggatatcggt ggatacgatc cccctcgtcg gggagcagaa
    22021 cctggcgctc gccgtggacg cggtggcccg gcttccccgg gcatcgatcc tggtgctcgc
    22081 cggatcgctg atgggcggcg aggtctcgcg ggccgtggac cgggtccgtg gagcgggcat
    22141 cccggtgatt gccctcaaga tggccgggag cgtcccgcaa catgcggacc tggtcgtgac
    22201 cgaccctatt caggcaggcg tctttgccgt gatgcacgtc agcggcaagg cagtcttcga
    22261 tatcaaccgc gtgcgtgggc gtgagttctg atatggattc tatcgatatg gcgattctgg
    22321 tgctgcagag ggatgggctc gtcgtctatc ccaccgagac ggtctacggt ctcggggcgg
    22381 acgctctctc cgaggatgcg gtgatgcggg tctacgaggc caagaaccgg ccggtcggga
    22441 agccgatatc ggtcgcggtc tcggatatgg agatgctcgc cgccgtcgcg gtggtggacg
    22501 atgtggcgcg agccttcatt gagcggtttc tgcccggccc ggtgacggtg atcctcccgg
    22561 cgaagtcctg cctgccggca gtcctcacgg gcggcaccgg tctcatcggg gtccggtggc
    22621 ccgacaacga gctcgcgctc gagatcatcg cccggctcga ctctcccatc acggccacca
    22681 gcgcgaacat gtccgacgac atcccgccga cgcggcccga tgaggtctct gttccccacg
    22741 accacctggt cgacggcggg gaactttccg ggacgccgag cacggtggtg gacctgaccg
    22801 cccggcgtat cctccggaag ggtgcggaat gggaagcagt ggaagcgttc ctggaaactc
    22861 tacggtgatc tatgccatcg gtacgactcc gtgatttcat cgaggaccct catggctgtc
    22921 tctatgcggt ctcgaactac gataacaccg accggatagg gtgcatcctc cgctacgtcc
    22981 ccgatccaaa cggggaacgg acaaacctct ccggcaggcg gtaccggaag tacgattttg
    23041 ccgagtcctt tgactggatc cgggagcatg agccagaata cctggatgcg gtccaccggg
    23101 tgcctcactg cgatgtggtc agggtataca aacccgagga ggagatcggg aaggtggcgg
    23161 cgcggagcgc ccgtgtgcgg aggctcctct cccacctgga tctcccggcg ggctcgttcg
    23221 ggtgcacggg ctcgctcctc tgcggcctgg agaacgccgc ctccgatatc gacctcgtcg
    23281 tctacggcga cgcctggttt gtcgcccagc gccagctccg gcacctggtg gaggccggga
    23341 tgatcccggg gatgagcacc gcgatgtggc ggaaggtcta cgacaaaagg gtgccggaga
    23401 tctcgttcga cgccttcgtc ctgcacgagc agcggaagtg gaaccggggg gagttcgagg
    23461 ggacctactt cgatctcctc tacacccggg cctatgggaa cctggacgcg gtccccgccg
    23521 gcatggggac ggtgctttgc cgggcaacga tcgaggccac ggtcacggac gcttctttgt
    23581 ctttcgacag tccggcggtc tacgccgtgg accacgacga gatatcccgg gtcctctcct
    23641 tcacccacac ctactcgggg caggcgctcg ccggggaggt catcgaggcc cgtggcgtcg
    23701 tggaggagca cggcgacgag cggtggctga tcgtcggcac gacccgcgag gcgaagggcg
    23761 aatacatcgt ctcgaagacg ctgctcgaga acgtttagaa gaggtccgag agccgccgcg
    23821 gcccggcggt gcaccgctcg aggtgggggc agccctcgca gggagcccgg agaggccgca
    23881 gcgggatctc gcccgcgacg acgcgttttg ccgcccggat cgccttgatc atctcccgcc
    23941 ggtctctggg ctggggctcg acgaaccggc aggtgccgct tgcgacgtac tcgacgtaac
    24001 cgccggcaac ggcgcgcccg agggtctctt tgaggcaggc gacgtagcag gcgacccgaa
    24061 ggcggtcggt gccgtagacc ccggccttcg gggcggtgct cgaccgggcg atggcgaacg
    24121 tgccgtcctc gaagaccttg tccacccttc cccggatgcc gagggtgtcg gactcgacgg
    24181 cgaggtcggt ctgaaccggc gtcttccagg tggtctcccg gcaggcggcg acgcactcgt
    24241 cgaggagttc ccgggactcc gccggtgcgt cggggaggac gcagaggacc tcttgccaga
    24301 tctgctccgc ctcaaggagt tctccgaggt gcgtggagac ctgcttgcag acggtgtagc
    24361 ggggcgactc cgggtgctcc tccgaccgct cgaagtagta gcgccggggg cagatgtggc
    24421 aggcgacgac gccggatacc gggatgtact cattcatgcg caaccgatcc cgtttgacag
    24481 gatgtatgcc ctcccgctat ttagcgccgt acgctctgca ccggtgcggg gtcccgggcg
    24541 gcccggaggg gagatgcggc tggaagacca acccttatgc cggagtggat cgtagaagat
    24601 gggtatggct aaccgcgaga tatcggtcaa catccccggc gacctcgacc cggtctactc
    24661 gaaccggatc caga
//
LOCUS       DAJB01000024            5409 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_117328, whole genome shotgun sequence.
ACCESSION   DAJB01000024 DAJB01000000
VERSION     DAJB01000024.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_117328"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          935 a         1662 c         1749 g         1061 t
ORIGIN      
        1 ccgcgagccc ggcagagata aatgatgagg ttatgcacca tgaccccgcc ctgacgctgc
       61 gagcctgtct gcaagctgca ggatcagggg ttttaacagg ggggaatacc accccttctc
      121 gatgtactgc gggtagatgc agagccgttc ggagaggtcg gcgtccccgg ccaccctctt
      181 gagttcttcg agttgcggcc aggggtgttc ggggttgacg tagtcgatgg tgagcgggga
      241 gaccccaccg aggtcgtcca cgccgcaccc gacaaggtgg gtcgcgtccg tgaggttcgg
      301 gggtatctgc actgcgacat ctgccgggag gacctcccgg gcgagggtta tcgtcgcaca
      361 gatctcgctc gtgtcgggaa ccggtgcatc ttccatcggt gtcgccggct tcgggcagaa
      421 gttctgcacg atcacctcct ggatatggcc gaaccgtcgg tggaggtccc ggatgacctg
      481 gagcgactcc tcgcgatcct ccatcgtttc gccgattccg accaggatgc cggtcgtgaa
      541 cggtatcgag agtctgccgg cgttctcgat catctcgatc cggaccgccg gatcttttcc
      601 gggagagtcc ctgtgtgcgg cgacgtcggc ggtcgtctca agcatcagcc ccatgctcgc
      661 gttcacctcg cggagccggt cgagttcttc ataggtgagg atgccggcgt tggtgtgcgg
      721 caggagcccc cgttcgatgg cggcaagaga gaggtcgtag acgtagtcga ggatgtccgc
      781 gtagccgatc cgatcgagca gggcggcaaa cccgggcact gcgccgggcc gctccccgaa
      841 ggtgaagagc gcctccgtgc agccaaggtc ggcacttgct tccagggtcc ggatcgcttc
      901 gccgggaggc atgatgcacc cctcccgtgc cggcgtccgg aaacagcagt agccgcaacg
      961 atttgcacag actgttgtca gggggaggaa tgcgttcctt gaaaaggtga tcacgcggcg
     1021 gtgcatgagt atatgtcggc actctctcac atgaacttta gggaacgacg ttcccgaagg
     1081 gcatggagtt tgagaaaacg cgaagcgttt tcgagcagcg acgggccgaa ggtgcggggc
     1141 cggagttcgg gcactgccag gtgtgaggaa cggtacatgc gaagaatgag gttcttcgag
     1201 gacacaaaaa cgcgaaggaa ggttctatac aggtgggttc tctacgatgc ctccataaat
     1261 gcccggttgc cgccggggaa tgcgatatga caggataagc agctttcgat ttgacccgcc
     1321 cggggttctc cggcgcacat ccataacgat gcctttttga tactgccctg acaacggttt
     1381 cacaatgtac tttcacgcgc tcatcccctt taagccagta aaccccaaga cgcgcctctc
     1441 ctgcatcctc aaccaggagg agcgggaggc gttcgcccgg gcgatgcttg aagacgtgat
     1501 cgcctccgtg ctgaagtccg ggtgcagtgc caccctgctc tgcacccacc ccttcaaaca
     1561 cgagaatgcg ctcattgccg tcaggaagga gtcactcaac gaagcgatca actgggcgct
     1621 cgggcagttt cactgtccgg cgctcatcat catggctgac cttcccctgg tgacggccgg
     1681 ggatatccag cggctgatcc gcaccgagaa ggatatggcc atcgtgccgg gccggggcgg
     1741 cgggacgaac gtcatattct taaagaagcc gaaatgcttc cacgctgact tctatggcgc
     1801 aagtttcctc gaccacatgc ggatcgcagg agattgcggc ttctccgtcg aggtgatcga
     1861 ctcattccgg atgtcgaccg atgtcgacga gaaggaagac ctggttgaga tactcatcca
     1921 cggaaaaggg agaaagagca gggaatacct ggagagttca gggttttccc ttgccatcga
     1981 cgagaaggga cgggtcggtg tggagcgcga cgcccatgaa gagacactct gaagcattga
     2041 tcgtggcgac cccggcgtag tcgccgacag gaagcccgat cccccgcacc acggctgcgg
     2101 ggacgcactc gtctgtctcc cccatcacca gttcggctgc tgatgcgatg cagtccgcca
     2161 ccgcccgctg ggtgacctcg agttcacggc cgaagagatc ttttttcccg cgctcgtcaa
     2221 gcaccgaggg gatgccggag cacccgatgg cgaccccgct gcacccgagc cgcatcgcgt
     2281 gcgtcctcga gtcgatgacg atgacgccga cgtcggcccc cgccnnagga ggacgaggga
     2341 gccgggcggg gcattggagg cgtcgatccc tgcgttcggg aggagtgtcc cgttcttcat
     2401 gcagagcagg aaccccggga tgcccccgac gacccggtcg ctctcccgga gcaccacctc
     2461 gacatggcga gggtccatat ggtattcttc agcgagccgg agggcttttg ccgacggctc
     2521 gatatcagcc agtctcgcga tccggccttc ggcggtggcc acggccgact ctgcaacgac
     2581 cacgacatcc cccgcctgga agccgcggca ttctgagttt gctgcggccg cgaggatgtg
     2641 ggttgtcata tcgtcgccgg cccggaccag cggggtcgca aggccgtata catggaatga
     2701 tggtgtcgtc atgcttgcct catcttctca taaacccgca ggaggtcggc ggtctccccg
     2761 acatcgtggc tccgcaccat ggccgctccc gcggtaacaa gcatcatcgt gagcgcgagg
     2821 gtgcctgcga gccggtcttc gggagctctc ccgagcagat ccccgatgaa cgtcttcctg
     2881 gagatcgcgg cgagcaccgg acgtccgaag gccgagaacg atccgaagtt ccggcagagt
     2941 tcccagtcgt cctcgctcgt ccgctccggg acccatctcc cgactgcggg gtcgaggacg
     3001 tattcgtcga tctcaaggcg ggcgcaccgg ttcacgaccg ccgcgagtgc ctcccgcgtt
     3061 gcggcaaggc cgacggcgtc cccgggctcc ttgaaactcg ccatcgcgaa taccggcagc
     3121 cccgagtcgg cgactgcccg cgcgtaccgc tcgtcggcaa ggccggcaat gtcattcacc
     3181 gcatggatct cgtggcggag gcagacctcg agcacctcca ggtgccgggt atccaccgag
     3241 agggtgatcc cggtcccgtc aagttccgag agggccgcat ccacccgttt cgcttcttcc
     3301 gcgacggtga tgtgaggaga gcccggggcc gtgctccgcg cgccgaggtc gatcatatcg
     3361 gcgcccgaat caagcatgac catagcccgg tcatagactc ctccggccgg ggtgtaggag
     3421 ccccggtaga acgactcggg gctgcagttg atgataccca tcaggcggac cggagcgtcg
     3481 ccgccgaccc ggaggcggtt tatggtgcag tgttgttgta gcatgtcctc aacttggccg
     3541 cccggaaggt gttctccatc agcgtcgcga tggtcatggg gccgacgcct ccggggaccg
     3601 gggttatcgc tcccgcccgc tctttcaccg cgtcgaagtc gacgtcgccg cagagtttac
     3661 cctgctcgtc gtagttgatc ccgacgtcga tgaccgtcac gccttccttc accatctccg
     3721 gtccgacaaa cttcgctctt ccgaccgcgc tgatgaggat atcggcgctt cgcatctcgt
     3781 cctcgaggtt cttcgtcttc gagtggcaga tggtgacggt tgcgtcggcg ttcaggagca
     3841 ggacggccat gggcctgccc acgtcgatgc tccggccgac gacgaccgcc cgctgtcctg
     3901 cggtcgggat tttatactcc tcgaggatcg tcatgatccc ctgcggggtg cagggagcaa
     3961 agaccgggtt cccggcaaga agcctgccga ggttgcaggg gtggaacccg tccacgtcct
     4021 ttccgggcgc gaccgcatcg atgacgcggg tggtgtctat ccgggacggg agcggcagct
     4081 ggaccaggat accgttgata tcagggtcgt tgttgagacg ggtgaccgcc tcgagcaccc
     4141 gctcggtgga ggcatcctcc gggagttcga tgccgaccga tccaatcccg acccgctcgc
     4201 atgcccggtg cttcatgcgg acatagagtt tcgagccggg atcttcgccc acgatgatgg
     4261 ttgcaaggcg cgggtagagc ccggactctt ctatctcctc tttgaggagc tcaagcctct
     4321 tctccgagac tgctttgccg tcgagtatca tgctacttca gggtgaaggg gatacttgct
     4381 cgccagagca ataacctctt ctctcacttc ggtgatcgcc ttcttggagg tcttgtccct
     4441 ggcgatgtcc ttgaccaccc ggccgatcca gtggccgatc tgcttcatct cctcctcttt
     4501 catgccgcgg gaggtgacgg caggggtgcc gatccgcaga ccgctcgtca cgaagggact
     4561 gagctgttcg cgggggatgg tgttcttgtt cacggtgatg cccgcctcgc cgagcgcgac
     4621 ctcggccgca aggcccgtga ggtgctcgcc gtttgcggag attccggtca ggtcgagcag
     4681 gatgaggtgg ttgtcggtgc cgccggagac gaggtcgagc ccttcctggc tgaggacatc
     4741 ggccatcgtc tgtgcgttcc tgacgacctg tccgcagtag tctttatacg cgggggtcag
     4801 cgcttccttg aagcagaccg cctttgcggc gatggtgtgc atcagcggac cgccctgcat
     4861 gcccgggaag atggacttgt cgatggcggc agcatcctct ttactgcaca tgatggcgcc
     4921 gccgcggggg ccgcgcaggg tcttgtgcgt cgttgtcgtc acgatgtcga cgacaccgac
     4981 cggggagtta tggtagccgg tcgcgcagag tccggcgatg tgggcgatgt cggccatgca
     5041 ccgcgcgccg acggtatcgg cgacttcctg gaatgctttg aaatcgatct cgcgggggta
     5101 ggcgcttgca ccgcagacga tcatctgtgg cttgacgatc cgcgctatct cctcgatggc
     5161 cgcgtagtcg agcgtctcgg actcggagtc gacgccgtag tggaagatgg agtaccagcg
     5221 cccggtgatg ttgaccggcg agccgtggga gaggtgaccg ccctgggtga ggctctggct
     5281 catgatcctg tccttgtagc cgaggtaggc aaagtagacc gcctggtttg cctggctccc
     5341 cgagtgcggc tggacgtttg cgtgctctgc cccgaagagc tgacacatcc ggtcgcgcgc
     5401 caggttctc
//
LOCUS       DAJB01000025            3796 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65550, whole genome shotgun sequence.
ACCESSION   DAJB01000025 DAJB01000000
VERSION     DAJB01000025.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65550"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          724 a         1215 c         1208 g          647 t
ORIGIN      
        1 actgtaccgg agtgctctgg gcaccctgat caacgccgat gtcaacggcg ctctgaacca
       61 tttacgaaag gtagccggtg attccgtgct tccacggata atcggtaggg gccgtgtcaa
      121 ccggcccgtg cgaataagga cgtcttttga accgtcaacc ttcgcaccga ttaaattgca
      181 gccctgtgcc ccacaagggc ccccgctgca agcccccggt tttaaccggg ggtagttgac
      241 gtacggcaga gcggtgccgt actcccagcc ggcagaggag tgaaaaaatg ccgggggcgg
      301 tccaccaccg ctgttaacgg tgtgagtccc gctcgacaag caaggaatcg cccggggtgc
      361 tcggccgtgg agcgttcccg ggcgactgga tgaaccagcc ggtccgggtg ccgtcgagcg
      421 tagcgggtga gctcctggag gtcaggaggc tgacgacgac catggcgaga gccgagagca
      481 cgagcgcata caaactgaag tgcaccggga gctttgctac aaactggtgc cagtagccga
      541 aggctgcggc cccgacgagc cccactgcca tgctcgcgat cgctccctgt ccggtcgcgc
      601 cgcgccagta gagcccggcg agcagcggga ccgcgaacgt cgcgagcatg accccgatgc
      661 ccatccagat cagccacgcg agcatcggcg gcgggttgat ggcgagcagg agcgtcccgc
      721 cggccccggc gacaaccgcg atcttcgaga cgaggagcac ctcgctgtcg ggtgccgagg
      781 gccggaagat gttcttgtag atgtcccacg caaacatggt cccgatggtg agcatgagcc
      841 ggtccgtcgt ggacatcacc gccgcaagga cgatcacggc aaagagtgcc catagcgcca
      901 cgttcggcat ggcatactcg acgcctgata tgaaggcgaa gtcctgggcg ttcacggcgt
      961 tggggagcac gagtttcccc tcctcgacga gcgaccgcac ggcaaacccg gtgaacttca
     1021 ccaggaacat cacgaccgcg tagatgacga acgcaacgag cggcgcccac ttgaagtagc
     1081 gggcctcctt tgcggccagg acgttgttga cgacgtgcgg cgcgcaggcg agaccgacca
     1141 tcaggaggat cccgaacgag acgaggaact ccggtgttgc ataggcgtac ggggcatagg
     1201 ccggactcgg gaaccagggc tcgacgaagt tcgggtcgac cccggcgagg acggtatcga
     1261 tgtgggcaag cccgcccgcg ctcgcgatga cgaacggcgc catcagaagc acgccgaaga
     1321 tcaggatccc gccctggaag agggtcgtca cggatacggc gtaaagcccc ccgacgaccg
     1381 tgtaggcggt gatgatgacc ccggagatga gcagcgcctc ccagtgcggg ataccaaaga
     1441 gccagacgag cacgatgctg atcgcggcgt actgccccac caggtagatc agcgagacga
     1501 tgatcccgga gactgcagag agcgcccgga gcgcccgcgg gctctcgaac cgttccgcga
     1561 ggtagtcctg gacggtcagg tagccccgct ccttcccgat ggcgttgagt ttcaccccaa
     1621 agagaatgat gcagaacgcg gccgcgagcg gcacgaagat ctgctcccag atccccggcc
     1681 agcccgaggt atacccaaag ccgctgacgc cgaggagggt catgccgctg cagatggagc
     1741 agacgatcag gatcgtgaag acccagaacc cgagcgatct gccggcgagg atatagtcct
     1801 cggtgttgtg gatcttcttc gaggcccagt tcccgatgcc gatgagcaca gcgaagtaga
     1861 gcccgatcag ggcgaacgtt atcagatccg ccattcaagc ggcctccctg aacaggagcc
     1921 cccatatgac gagcaacacg acgaccaggg cgacaacgcc gcccacgatg atttccgtat
     1981 gaataggtag cccgaagaac atgctatgcc agtatgaggc gtgctaacat ataacacgat
     2041 cgcaggagac cgggagaaca ccccgatgga acgacggagg ggttacaccc caaaaaatgt
     2101 tatttggaac tcttcacggt ctttgggccc cggaggagag caatcttccc cgtgcggacg
     2161 agttccttga tgccgtactg gcgcaggagc gtctccagcg cgtcgatctt gctggtatcc
     2221 cccgtcacct ggagcaccag cgtctttgcc ccgacgtcca cgaccagggc ccggaagata
     2281 tcggcgatct gcaggatctc ggcgcgggcc gggccggtct cggcagccac tttgatgaga
     2341 gcaagctccc gctccacgct ctcccgcgcc gtcaggtccg agaccttgat gacgtcgatg
     2401 agcttgttgg tctgcttcat catctgctcg acgaccccgt cgtcgccgtt caccacgatc
     2461 gtgatccggc tcatatccgg ctcttcgcag gtcccgacgg cgaggctctc gatgttgaac
     2521 ccccggcgcg agaacattgc ggcgacccgg ctcaagacgc ccgcccggtt ctccacgagg
     2581 acgctcaacg tatgctgttt catctctggc gcaccccgat catctcgttg attgcagcac
     2641 cggccgggac catggggaag acgttttcct cccgttcgac cctgaagtcc aggacgaacg
     2701 gcccgtcggt ggcgagcgca gtctcgatcg ccccccggac accgtccttc tcctcgacct
     2761 tgaggccctc gatcccgtag gcgttcgcga tcttcacgaa atccaccggc gggagttccg
     2821 tataggagta gcggcggtcg tagaagagtt cctgccactg ccgcaccatg ccgaggtaca
     2881 tgttgttcaa gatcacgacc ttgacgggga tgtcgtactg cgccaccgtc ccgaactcct
     2941 ggatgttcat ctggatgctg ccgtcgccgg cgacgtcgac gaccggcata tccggccggg
     3001 cgtagtgggc gccgatggct gccgggaggc cgtagcccat cgtcccgaga ccgcccgagg
     3061 tgatccaggt ccggggtttc gtgaagttat agtagagagc ggtccacatc tggttctggc
     3121 ccacctcgct cacgatgatc ccctctccct tcaggacatc ggagagctgc tcgacgacgt
     3181 actgcgggtg gaggcggccg tcgtcgctgt aaccgagcgg gtactgcgcc ttccagttgt
     3241 tgatccgggc gacccagttc gccgtctccc cgcgcttctt catccgccgg aggagcgcct
     3301 ggagcaccgc tttgacgtcg cccacgatcg ggacgtcgac cttcttgttc ttcccgatct
     3361 ccgccgggtc gatatcgatg tggacgatcg cggcgttcgg cgcgaaggtc tcgatcttgc
     3421 cggtgacccg gtcatcgaac cggacgccga cggcaagcag gaggtcgcac tccgtgacgg
     3481 cgtagttcgc cgactgggtg ccgtgcatcc cgagcatccc gannccccat cagggtcgtc
     3541 gtcaccggga tggcggcggt ctccatgaac tccaggagtt cggcggatgc ccccgagagg
     3601 accaccccgc cgccggcgta gatgagcggc cgctccgccc gggcgatcag gtcgagcgcc
     3661 ttgtcgatct ggcggacgtg cccctcgtag gtgggctggt agccccggag ggagaccgtt
     3721 ttggggacgg tccctccgct cttcatctgg ctcgtggtga cgtctttggg gagatcgacc
     3781 agcaccgggc cggggc
//
LOCUS       DAJB01000026            6816 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_57349, whole genome shotgun sequence.
ACCESSION   DAJB01000026 DAJB01000000
VERSION     DAJB01000026.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_57349"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    5924..6072
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6653..6670
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1415 a         1982 c         1936 g         1314 t
ORIGIN      
        1 accgcgaagg tgaacttcct cgacgccgtc atcgcgctgg agtgagagga ggtcttttgc
       61 ccccactctc ttccccgcgc cgggaggcct ggcaaccggg gcgtctcccc tacctgtggg
      121 gggcgttttg tcttccgggc ggtggtggat gttcctggag aggaggagat ccggagagta
      181 ccctcaccgg cgaagagggg atcggagcgg gaaaagtatc atctgccggg agcactcata
      241 tctgcatatt ctggagtgta cggcatggga gtgcaataca tctgtgatgc agaaggaaga
      301 aagactggcg ttatcgtgcc catcgacctc tgggacagcc tgaatgccga aaaacctgcc
      361 ggtgaacggg ttggggttcc ggatcctgga aaatatcggg gcatatacag aaacttaaac
      421 gtcgatctaa aggccgagat ccggaacctg cgggatgaat ggaaccggat atgagtcgat
      481 acctgctcga tacgaacatc ctcatctact accttgcaga tgccgttccg ccggcgagag
      541 taaatgatat cgaagagatg ctcacctctt catttcgtgt ctctatcatc acaaagatcg
      601 aatttctcgg gtggaagaag catactccgg aagggtatgc gatagccgag gacgttatcc
      661 gttctgcaga agtatacccc ctgatcgata caattgcgga ccgcgccatc cacctgcgtc
      721 gaacttgcgg gatgaagctc cccgacgcgg tcattgcagc caccgctctg gctcacgaat
      781 gtgtaccggt cacccgaaat gaacgtgatt tttccgggat ctgcacgctt gaggtcgtaa
      841 acccgatcga cggcgggtag atgcggcgca gtggtttcgc gtggtactgg cggtcagtga
      901 gactcagggc tgctcgcatc ttgtccgatg cagatgtcca gagcacccca tcaacaccag
      961 gagttcgttt gcctttgttt tgtgtgacga tccgcaccgc gaggagtttc gcggagtagg
     1021 aatgcgtcag cagatacctg agacgtttga ccagattcca ttttccttcg tgtgttgcct
     1081 ttgcaatccg ggtttgtagc ctgttaacaa tggattcacg atccccgccg agatcgtcgt
     1141 gagacggagt gagggggcga ctttctttcg tttcggcata cagagcggca atagtactcg
     1201 ttgtcattta actctatgaa tttcctttcc cgcccttcgn ntcctaacat aaactgacaa
     1261 gatgcaatag gtttccggtg tgatcgtcga agcagatcat atgtagtcac aggtgaaaca
     1321 tcctctggat gacaccacgg aacgtacccg ggacctgcat gctctgcagg gaaccggtga
     1381 gcaggagggg agctttgcgg cacgtcgagg gctgcctccg gtcgtcgggc tggcctgcag
     1441 gggaaaagcc gtcgctcatc atcacgatac aggggcggca cgacagatcc tactggctgg
     1501 tggttcttgc gcggcccgac gccacgctcg aagacctcga ctccctgatc cgcgacgtat
     1561 gggtggaatg ctgcgggcac atgagcgcgt tctccatcgg caaagagagt tacctgagcg
     1621 gcgaggacga gccgggaacg agcatggcaa tccccctctc cgggttggtc ccgccgggaa
     1681 gcacgttctc ctacgaatac gacttcggct ccacgacgga gctggacctg aaagtgatcg
     1741 gtgcaatacc cataatgcct ccggagaacc cgatctgcct gatcgcgagg aacaaccccc
     1801 cgccgttccc ctgcgacgcc tgtgggaatc aggcggaatt cagcctgttt gacgatgaag
     1861 acggtaccct ccgtttcttc tgccgcgaat gcctcgtctc ggccgaaccc gatccggagt
     1921 gcacggagat catcgagaac tccccgagaa ccggtatctg cggctacgtt gaggaccctg
     1981 atgccgcggt gtactggtat ccgccgggat ggaacgcgga ggatctcgca tccccggaac
     2041 tcgaaaggat tctggagaaa ctcccggagt tcgatccgga cgactgcata tatactgtcg
     2101 aagacccgcc cggggacgca gaggtgcagg ccatgataga tgccgtctgt gcggatatcg
     2161 gcccggagat tgaggcgttc atcgccgatg agcgggccgc gcacggggag atctatggtg
     2221 cgctggcgga agagaccgtt atggagttct acgtcttcac gtacggcctt cgcggaaaga
     2281 ctatccggga gtgggatgcc cggctcgtac gcacgtgcct gatcgaagag atggcgcaga
     2341 acccgatctt ccccgaggaa tggcttgaga acgccgtccc gatcctctgc cggttcctct
     2401 cctccatgga ggcggccggc aggctgcaga atgccgcgga actgatcgca gtactcaaag
     2461 aggcggaacc cgccttccgg gccgcggcca cgagccggga gaagtatcgc gacctgtccg
     2521 gtcgtatctt ccagcgggcg ctcgacgcag gggtcgaccc ggaggatgag cgtgccctga
     2581 tgaacttcgt ccagcgcgaa gttgtagtaa tggccgggat ggacccggac gatgcggatc
     2641 tcatcgaaaa aatcggaaat atattcgaag gcggcggaat cgcctcgaat tccggggatc
     2701 tgcgggtctc cgcgatcgtc tccaggtgcg aggaattctg tgcccggctt gaagacgaca
     2761 cgatcccggg acgttgcggg gccatcgtcg gcgacctggc cgcccacccg gcagcaccac
     2821 tctcgcgggg ggacgaactc ctctggagtg cggccgtcgt ctatgcagca tgccgggagg
     2881 caggtctgat ccggccggga aaggggggga ccccgttggc ccgggagatc ggttccttct
     2941 tcgatctcga accgtcatcg gttcggaaca aggtggcagc gctcaaaaaa tatctctcgg
     3001 atacgccgga atcggaagac tgaaccgagc cgggagcaca cgcgcaggcg ggatgcgaac
     3061 cggcgtacga ttccggttcc cgtcgacggc aaccccttcg ggtgcgtcgg gtataccggc
     3121 tccgacgggc agccggtcgc tgctgtcgtc cggaaccgcg agcactacac cgcgaaggtg
     3181 ggcttcctcg acggcgaggg caagcaggtg ggcaacgtct cgctccagtc cccgacgatc
     3241 gccgccttcg aggcgaacgc cgcggaggct ctcgcgaacg cggccctcgc aaccgcgatg
     3301 ctccggtagg aacgacgttc cgttggaacg gagttcgagc accagaggtg cgagttgcga
     3361 agttctgcag gagcggagcg ggtgcaccga aggtgcgact cgcgatgggc ggggaggccc
     3421 tccgcaacgg ccccggcgag acctactacg cccagctcaa gtgccacgac ccgtcgggtg
     3481 attcgaagcc ttcggcttct cacgctcctg ccgctcacta tgctcccgcc agtcacgact
     3541 cctacgtcac cttcacccgg acgaccgtcc ggctctcgtc ctaccaggac gacgccatcc
     3601 ggaccgcggt cgagacctgg gccgacgccg tcggcgcact ggcgtgaagg ggattctttc
     3661 cccttcactt tcaacctgcg cacggccacc ctttatacca ttccccttct ccagactgga
     3721 tatgaaatgt tcccctcccc tccgctacga ccagatcctc ttccgcgacc gtgaggtctt
     3781 cgagttcacc cacatgcccg gcaatcaccg caatggggtg gacacacagg agacgatagg
     3841 caagggcctg cctgagaacc cggagctcat gcggatattg caggacaggt tgatcgagga
     3901 gaagatcaag cgaggtgata cttgcaaggg tgctccacat gaaattcttg gtccataggt
     3961 agccagcccc atgaatgata tctatcacga aagagatcga tccggatgga agcagttgca
     4021 ctggaagcgc agcatagcga ggagtgcatc gccggtagaa agcacttgac agtgataaag
     4081 ggacaaacag accgttaata tccgttcaaa ctccctcacc ccccaccgag cacccgcaca
     4141 atcaccgcca ccaccccgcc ggccccgtcc ctcacctgcc cattccagac aggtattccc
     4201 gcgaaagccg ggcgatatcc tccagatcaa agacctggta cccgtcctct ctcagatcct
     4261 ccttaccttc gatgctcttt gctaccaggg caaaacgttc gttcctgctc tcgttccgcc
     4321 atctcacctc acaagccttc tcccggagtg ccgcaaggat gccgcgggcc tggcggcggt
     4381 tgagagcaga ccatttacat tcacagaaga gggcatcccc ggagacctca ttcagaccta
     4441 cgagatcgat ctccgcctcc ttctgccacc accgtcccag tcgcgaatac gaggagccgg
     4501 aaaacaggat ccccctgcga acgaggtcct cggagagttg ttcgaacatc agtccgcaat
     4561 agggcgcgat ctcttccctg atagaggcga gcacctcccc actctgcccc gtctcggtct
     4621 ccgccctccg gggatacacg aaacggaacc agaacatgag gtagggatcg gtcagccgat
     4681 aatgccttct ccgatagccg gcatgggcgg tcaccggaac ctcatctccg atcagatgca
     4741 tacgcgaaag gacggcaagg tacttggaaa ccaacccttt atcaagcccg gtctcatgac
     4801 agaggggtgt cagggcggtg taccccgcag cgagagcgcg gagaatagcc atgtaattcc
     4861 ctgcttcgcg gaactcatac tgcatcagga tttccgcttc agagtaaagg tatgctcctt
     4921 tccggaggat ctgctcctcc acgttctccc agaatgttcg gtcggggcga aacagacgga
     4981 gataggcagg aattccaccg atcacgcacc atgtcatgac gagatcctcc tcatcgtagg
     5041 ggaggaatcg gcgaagatag ggataggaga gaggctccag ctgccactgc cctgtccttc
     5101 tgccgtagag cgggcttgtg taccccagca cttcggtctc catcgtgctg accgatgaac
     5161 ccgatatgac gagcatcacc ggctcccggg agagtaccgt atcccacacc ttctggaaga
     5221 gggaaggcgt ggcctggtcg cgtgcgatga ggtaggggaa ctcatcgatg acaatcacca
     5281 ctttcttccc cggaggaggg gaaaaattcc ggtgcctgac cagtgccttt atgacggtct
     5341 cccagtcggg atatctactc cgctcaaaat ccggatcgtt caggaactcc cctgcatagt
     5401 gggagaaatc ctgaacattc cttgccgtcc cttcttctga ggccaggaag taaaatcccg
     5461 ggacggtctg caggagtcga gcaagaagcg tcgtctttcc cacgcgccgc cttccataca
     5521 ggatcaggca ttccggaacc ggggaacaat accgatcggt gaggaactgc agttcacgtt
     5581 cccggtctac aaaggattgt tgatgcataa gtatactact ttgtagtcaa caatgcactt
     5641 aaggcgatcg gtaagcgccg gtggtccggc cggacatgac cgctgcatag atgggcttcc
     5701 ccttaccggg gtttacggtc acgtgcccgt agccgggcgg tatgaggacg aactcccccg
     5761 cctttgccgt gacagcgaca acgtcacgga gatcgcttcg ctggaggatc gtctcgtaag
     5821 accgagctcc cgggtggtct ttgcgtcggt catcctggta atcctcacgg aggacagtag
     5881 gacggggaag agaaaaatcc tgaccgtaac gattatacgc agannnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nntgaactcg gtctttcctt cctgcagggt tcctttacag aacgtgcatc
     6121 gctctggaat cattcctgtt tcctccttgt tcgcgtatcg atccaatgct cgtcatccgg
     6181 catgtaggcg gtgatgaccc ggagatgatc atcgcagatc ccgagtacta catgatacac
     6241 gtggtcacct ataaagccaa gcatgagcag ggagggacag ggctcgtcat cggaatatga
     6301 ttcgagaatc tcgccggccc tgattgccga aaggaggtcg tcggtggaga tgttgcgctc
     6361 aaacatcctg acccgggcat gatgcgagac gatgaccgaa tcccggtcta ccaacctgat
     6421 gatctcttct ttgctatgat ccacgtcttc ccctgtcgat cttgtctcct gcacactaaa
     6481 accttcttag ccgtgaggaa ctaccaacca tggcagactt cgtgcagaag accgtgaaca
     6541 aaacggcggt ccgggacctc gccgtcccga tcgccaccgt aacgtcgttt aacaacctca
     6601 tcgagagcgt catcgaggac aaccccttcg ggtgcgtcgg gtataccggc tcnnnnnnnn
     6661 nnnnnnnnnn cggcgagggc aagcgggtgg ggaacatctc cctgcagtcc ccgacgatcg
     6721 ccgccttcga ggcgaacgcg gccctcgcaa ccgcgatggg cgcatccgat gccctccgca
     6781 acggccccgg tgagacctac tacgctcagc tcaagt
//
LOCUS       DAJB01000027            5454 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_99753, whole genome shotgun sequence.
ACCESSION   DAJB01000027 DAJB01000000
VERSION     DAJB01000027.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_99753"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          794 a         1890 c         1773 g          997 t
ORIGIN      
        1 gcggcctacc cggcctccct cctcctcttc gccctcgcga cctggtactg cggcctcctc
       61 cgcatcccgg tcgccctcgc cctcctgatc tttctgggtc tcggggccta cggcctgtgg
      121 cggcgcgagt accggctccg ggagatccgg ggtctcctcg cctgggacgc ggtcttcctg
      181 gtcggatttc tcttcgcgct cgcggtccgg ttcgtgaacc cgcccatcgg ctacttcagc
      241 gagcagtaca tgaaccacgc cttcctcgcg agcgtgatcc gggagcctgt ggtgccgccg
      301 ctcgacccct ggtttgcggg cggccacctc accgtctact actacctcgg ccactggctg
      361 acgggctgcc tcgcgatcgt caccggcatc ccttctgagg tggccttcaa cctggttccg
      421 gccacggtct acggcgcctc attcgtgacg ctctacgcca cggggagcct gctcctccgg
      481 cggtggcggt gggtagcgct cccggtcctc ctcctcgtcc ccccatcggt cccctggttc
      541 ctcgctaccg gtgccggcct ctatgaggcg ctccaggaga ccaactggat cgtcgccggc
      601 gcccggttcg agtttccggc cttctccctc ttcctcggga acgtccacgc cttcgagatg
      661 gccgcgttca accagttcct cctcatcttc ctcctcgggt ttgcgtggtg ccggtggagc
      721 gggctcgaca cccggggccg gcggggcctt gccctcctcc tcgcggtgag catcgggtcg
      781 atgccgctcc tctcctcctg ggacgccctc gtctacgggc cgcttgtcgc cgtcttcctt
      841 ctcgcgctct ctctccgcga gcgggaccgc tccactctca tggccgccgc cgcggtcccg
      901 ggtctcgcct tcctcctcta cctcccctac tacctgcagc ttgagcccgc gggtatcggc
      961 gggatcggcg caggcttccc ccccaccgac ccgctcgcgt tcctcgcgat ctggggcgga
     1021 ttccttggaa tcgtctacgc cgccgtcgcc cgggatatac ggcggttccc ggtcctgctc
     1081 gccgtcgcgc tcccgtttct cgccaccggc tacgtcgccc tcgggctcgt cctggtgccg
     1141 ctcgcctacc tcctcctccg gcgaaaacac gccttcccgg acctcctctg cgtcgccggc
     1201 ctcgcggtga tcgctttctg cgaggtcttc tatttgatgg agatgctcgg cggagattac
     1261 agccggttca acaccatctt caagttctac ttcgacgcct ggatcctcct tggcaccggc
     1321 tccctcctcc tcgccggggg gtggcttgca gcccggcggc ccatactccc ggcggcggca
     1381 ggaaagtggc ttgcggccct cgcggccctc gctctcatcg ccgccccggt cgccctcgac
     1441 gtcgatatcg gccggggcct cctcgggatc gactacccgc cggcgggcta caccaccctg
     1501 gacgggctcg cctaccttga ggccacgcgg cccggggagg ccgcggcgat cgattacctc
     1561 cggacgctcg acggcggcca cgtgctcgtg gaggcggaga acggtgatta tgggtattac
     1621 tcgcggatct cgtccttcac gggcatcccg acggtcctcg ggcagatcag ccacgagctg
     1681 acctggcggg ggaacggcgc gtggtatacc gagcgcccgg cggagatccg ggcgatctac
     1741 gaggactcgg agcagaccct cgcgctcatg gcgaagtaca acgcgaccct cctctacgtc
     1801 ggcgaggccg aacgcgagcg ctacgacgtc cggctgccgg accacgggct cgtgccgatc
     1861 tatgacaggg gtggggtccg gatctacgag cgggatgggt aacctttatg actcccggtc
     1921 acagggtggg gctcatggta cgagcaaccg tagtcctggc gcccgtcacc gcggaggtgc
     1981 gagcgtgacc gttgagatcg cggccgagaa cgtggccttc gacacggaga cgatcacggt
     2041 cccggcgggt gcggaggtga cggtgaactt cgagaaccgg gacggcggcg tcccgcacaa
     2101 cgtcgcggtc taccgggact cctcgcgacc gaagcgatct tcgtcggcga gatcgtcacc
     2161 ggtccggcgc aggtgacgta taccttcacg gcaccgtcgg agcccggcac ctacttcttc
     2221 cggtgcgacg tccacccctc gatgaacggg gcgttcgtcg tggcgtagcg ggggtcgccg
     2281 gctccgggtg gactgatatg gtttagttat taacccgtat cccatgcagg tgcctccccg
     2341 gccgggaggg cgccgcagtc gaggtacatc atgccggaat acactctcgc tccggtcggc
     2401 gagtccgacc gggagggcat cgtcgatatc ttcaattatt acgtcgagca cacctttgcc
     2461 gcctacccgg aagagatggt gccgtacgcc tttttcgacc tgatcctcgg gaccgcccgg
     2521 gactatccgg tcgtcgcggt acgggacacc gacggcgaag tcgccgggtt cgggatgctc
     2581 cgtccacaca acccgatgcc ggcgtttgcc cggaccgccg agatcaccta tttcctgcgg
     2641 cccgacctga ccgggcgggg gatcgggtcg gcggtgctcg gctacctgga ggccgagggg
     2701 aggaagcgtg ggatcgcctg catccttgcg agcatctcct cgtgcaacga ggggagcatc
     2761 cggttccacc ggcgccacgg gttcgccgag tgcgggcggt tcagaaacgt cgggtccaaa
     2821 cgcggggtcc tcttcgatac cgtctggatg cagaaggaga tctagaggac gacgcctgcg
     2881 atgatcgccg ccgcatagac gatcacggtt gcgtggatga gcgggagcgc ccgcatcgcg
     2941 gcagccgggc tcttccccct gaggatgagg tagtttgcag ccccgaggag gacgagtccc
     3001 acggcgaacc cgacgatggc ggccggtccg agggcaaggc cgaagacgag cgctatcgcg
     3061 gcatggacgg ccgagaagcc gagggtccag acggccgccc ccttcatgcc gtagaggacc
     3121 gaaaccgtcg ccatcccccg ggcgcggtcg tttgtgacgt cggcaatgtc gtttgccgcg
     3181 aggtgcgcga gcgtccaggg gtagaagaag aggaagaaga ggagggcggt cgtgtcgggg
     3241 ctgccgtaga cgaggtagcc ggctacgggg aagagggcga agtccgtccg tccgacgacc
     3301 tgggcgatcg gcagggtctg gtcccgcttc ttggtctggt aaaagtactc gaccgcgtag
     3361 gagtagccca tgatgccgag gacatagagg ttgtggggtg cgggcagggt cgcggcgagc
     3421 gcggccgcag acccggcgag ggcgaggaag acggcgaagg ccgcccgggg ggagatctgt
     3481 ccttctggta tcggccttac cccgaacggc cgccagtagc gggtaagcga gccgtcgacg
     3541 tcccgccggt cgtactcccg gtcgacgtag tcgttcaaga cgaggccggc ctcgaaccca
     3601 aagagtccga cgagcgccgc ccggacgacc aggtcccacg agaacccgcc gtagttctgg
     3661 aacgcgagcg cgagccccga gcagaagagc aggggccaga cgaagaagaa gtgggctcgg
     3721 gtcaggtcgg cgagcgccgc aagggtttct ctcatgggtg gtacgtcatg ggttctacac
     3781 ctgtgctcaa aaaatgttgc ggcagagtta agtccgtagc cgaaccactc cccaccggtg
     3841 ataagcatgc agacctaccg ttgctcgata tgcgggcata tctacgaccc ggaggccggc
     3901 gatacggata ttgccccggg aacggcgttc gagaacctcc ccggcgactg gcgctgcccg
     3961 gtctgcctcg cggagaagag caagttctct ccgttcgtgt ctcctctcgc gaggctcggg
     4021 ctctgagggt ccctgtccct ttctccagcc ggccgccgac accgtcgctg cggtcctgca
     4081 ccgggccgag gcggaagcgc cgcccgggag gactgcataa gaagacggag agcgtagagt
     4141 acctggctgc ggccccggca ccggggcagg cggcgggagt tctctatggt ggatacctta
     4201 ctgccgggcg ctgttctgct cgtctatgct ctggtgaatg gttcttcgtt cattgcctac
     4261 tatcgtgaca agaagctcgc agagcggaac atctggcgga cgccggagcg gcggctcctc
     4321 attctcgcgc tcgtcgggcc gttcggggcc tttgccgcga tgcggctctt ccggcacaag
     4381 acacagaagg cgaagttccg gctcgtcccc ctcttcctct gcctgcacct cgtcctcttc
     4441 gccgcgctcg ctctggggtt cgcgtgacct gtccgcaccg gaaccaattg acaaatggca
     4501 aaagttaaac cctctctccg ccccactctc cctgtatggc atacggctac ggcccgctcc
     4561 cccgtccggt cgaggagact ctcctggcgc tccaggcagg gctcgtgcgc gagtaccggg
     4621 cggaatacct ccccgtccac cggcggtcgc cccggagaat gcggaggctc cggcggatac
     4681 aggggtggat ccgggacatc ggccgcctgc tattgcagat cgaccgggtg atgcaggaga
     4741 tcctcccccg catcgagcag gacaccgggc ataccttccg gggcccggac gggctcgccc
     4801 gggtcctgat ggacccctcc acaaaacggc tcttctgggg gatcctggcc gggttcccgg
     4861 aagaggcgct cccgatcccg gcgaaggacc tcgctcagct cggaaccttc tccgatgacg
     4921 cccgggccct cgcccttatc ggtgatgtca ccctgcggct gaaggtcctc tccggcccgc
     4981 acgttcaggg aggagatctc gcggccctct gcgatcgatg gggactccac gagagccgga
     5041 tcggttttgc gtgccgccgc tgtccggccg gcgacatcct cgtgcgggag aaggaggtcc
     5101 tcgcctgggc cgtcctcggc ctcctctacg tcgacggcgg cacggatgcg ctgcgggccg
     5161 tggtgccgct gtttgcgtac ggtcctgaga tgtaggggcc gaagaaaggt atacattcct
     5221 tctggccccg gaagactctc atggacaccc gtgccctccg gcagttcatc cggctcggcc
     5281 ggttcccctt cctcctctcg ggcttcctcc cgttcaccgc cggggcgctc ctcgcgcttc
     5341 tcctcggggc ccggttcacc ccggcgcagt tcctcctcgg ctacgcggcc atggcagcgg
     5401 cgcacctctc cgtccactac agcaacgact acttcgacgc cgacgccgac cggt
//
LOCUS       DAJB01000028            2833 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_100761, whole genome shotgun sequence.
ACCESSION   DAJB01000028 DAJB01000000
VERSION     DAJB01000028.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_100761"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          466 a          919 c          862 g          586 t
ORIGIN      
        1 gcaccttccc cgcgtagaga gccgacccga gcaccgcccc ggccgctcct gcttcccgga
       61 gcgctccgac gtcccgtgcg ctcgatatcc cgcccgagat gacgaccggg accttcactc
      121 tcgcgaggag atccgtcacg ggtgcgagtg cgatcccctg ctggagcccc tctacgtcga
      181 cgttcgtgta gaggagcgat cctgcgccga ggttctcgaa ccgttccgcc caggagaggt
      241 aactcccggc agggcgctcc catccctcga tcatcacctc gcctccccgg gcgtcgatcc
      301 ccgccatcac acgttcactc ccgaattcgt cggcaagcgt gcggactgtc tcggggaccc
      361 tgatggcctg ggtcgagagg atcacccggt cgatgccggt atcaagccat ccccgagcgt
      421 cctccacgct cctgatcccg cccccgagtt cgacaaaagc gctggtctcg cgcgtgaagc
      481 cccggatcag gtcggcgttc ttctgcgccc ggccaaacgc cccgtcgagg ttgacgatgt
      541 ggagcccgtc cgctccttca tcgagccacc ggcgcgccca ggcggccggg tcaccgtaga
      601 ctgttgcggc ctccggtcgc ccctgcacga gctgcacgca tcgcccgtcc aggacatcaa
      661 ctgcaggata gatctccatg ctctcagcgt atgatccgtt cgataggcat gactaatatg
      721 tcttttgcgc cggcccgctt caactggttg atcaactgat agacccgctc ctctttcacg
      781 acagcgtgaa cggcgaccag gttctcgtcg gatgccacgt ccatcaccgt cgggccggag
      841 aggccgggca gcacctgcct gacatcggat agagcgctcc tgtggacgtt catcatcagg
      901 taacactgcc ctttcgcccg tatgacgctc tcgagagcaa ggatgatctc gtcgaccttc
      961 tcctttttct ccgtgagaga ggcggggttc gccaccagga ttgtcgtgga ggtgaggacc
     1021 tcgtcgatga cgcggagatg gttcgtccgc agcgtcgtcc ccgaggagga gaggtcgacg
     1081 atagcgtcgg cgatcccgag gtgcggcgtc gcctcgcatg cccccccgac ggtcaccacc
     1141 gtcacggcga tcccgtgccc ggcaaagaac gcgcgggtga ttgcagggaa ctcggtggcg
     1201 atctttgccc ccgcgagatc ggcgatggtc tcgatgccgg actcctccgg cacggcgaca
     1261 acgagcgtgg ccctgccggt ctgcaggtcg agcacctgct cgaccacaga gccccgctcc
     1321 atgaccatat ccctcccggt aatgccgagg tcggcaacac cgctcgccac gtactccggt
     1381 atatcgatcg ggcgggcgaa gaggatctct acgttcgggt cgcgagtcct ggtgatgagc
     1441 ctccgctcac ccccgtcgac cagatggagg ccgctcttct cgatcaactc gttgatcggc
     1501 ccggcgattc tgcctttgtt cgggatggcg agccgaacga accccggtcc gggcccgggc
     1561 tgccgggata ctggtttact catgggttac aacaactccg gtggaggagg tcagaaggta
     1621 gagagttctc ccctgatgac cttctcggtg atggtggtgg tctttgccag ccacccgtcg
     1681 atgatcccct cgatatcggg cttaacagag ttgatgctaa cacctggttt cgtgaggacc
     1741 tgggcgcttg ccacgtgcgg ctggtcgatc gggtagccga tctgggagag catacggatg
     1801 tatatctctt cgatgccgtc gacctccgcg acacagtcct gtgctatctg cgtcgcgagg
     1861 aggttgtaga tcttgccgat gtggttgatc gggttcttgc cgctcgtcgc ctccatgctc
     1921 atggggcggt tgggggtgat cagcccgttc gcacggttgc cgcggccgac ggacccgtcg
     1981 tcgcccatct ctgccgaggt tcccgagacc gtcaggaaga cgctgccgcc ctcgaggtcg
     2041 tcggcggtgt tgatggcgac attgaccgct ctcgtggtga actgctttgc gaccagagca
     2101 atctgttctg tcagaacgtt cttctgctcg atatattcgg cgatgctcga gcagtagcgg
     2161 tccacgaacg ccatcgctat ggtgagggtg atggtatcgc cgtcacgcag gcacatgatc
     2221 ttgcagtcct ggccgatgac gggatacttc aggcgaagtc tggtgtcgat gaacgcagca
     2281 acaccccgaa caatgctctc cgcctcactg aacggcgcgt gtccgacacc gaaggaggtg
     2341 tcgttcgccc gggggacctt cccctcgcag gaccggaaga cgtcctgcag gtcggtcgac
     2401 ccctttccca tacggcagtc gacgatgacg tcgcggtcca tgttgatggc gggaaggatc
     2461 tttctgatat agtctcgtgc cgcctcgacg gcgattgcgt ctgtcgggat gtttgcgccg
     2521 ttgaaggtcc tggttgcccg gcccgatatg aggaagtaga tcggctttgt gatccggccg
     2581 cccccgaact tcgggagcga ctcccctgca acgacctcgc cctggtcggt gttgtggtgc
     2641 aggacgctgc tgcactcttc aaggtacgat ctgctgagtg cccggctgat cgattctgcg
     2701 ataccgtctg cgaggctgtc cgggtgcccg aggcacttgc gctccaccag ttcaatccgc
     2761 tgcttctcga tcgggatctg gtcaagccct tctacgctga tgttcctggt cataataatc
     2821 aggcaggaga ccc
//
LOCUS       DAJB01000029            2691 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_92513, whole genome shotgun sequence.
ACCESSION   DAJB01000029 DAJB01000000
VERSION     DAJB01000029.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_92513"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          677 a          772 c          798 g          444 t
ORIGIN      
        1 ggtcaggttt gcccgcccca aaagcctctt ccagcttgtg cggatcgagg acgaactcaa
       61 gcggactctc gggagagacg tggacctcat caccgaaaac gccgtcagtc cctatcttgc
      121 cgatgcagta caccgtgatg ccgtggtgat ctatgaagcc aaaggacccc gcgtatctcc
      181 gccacatcct tgatgcaatc acaagcatcg aggagttctc agaaggcatc tcgtcggtgg
      241 aggacctcag gggtcgccgg gatcgagcgc atgctgacca tcatcggcga ggcggcaaag
      301 atgatctcgc cggagctcgc gcaggagcac ccggagatcc cctggaggga agcggcagga
      361 atgcgaggat catccaccat tactttggcg tggattacga agcaatattc ctgaccgtac
      421 acgacgacct cccggtgctc aagcagggaa tccagtcgat cctcaatgag gccaaccgca
      481 gaaacacccc cggcggaaaa tgagaaaccg tattcggtga gtaaaagagg gtcgatgccg
      541 gaaaccagga ggctccccga ccgtatggcc ccgtgcggcg tctactgcgg agcatgcccc
      601 tcattcggga aagggtgcag ggggtgcggc tcggagaacc gcaagcagcg gcgcaccagc
      661 aagtggaact gcaagatccg ccagtgctgt tttgagggga aggatcttgc actctgctcg
      721 caatgcacgg actttccctg caagctgacc gacaaactcc aggggtccta cccgggcaac
      781 gagcgattca ggtaccgcca tgagatatac gagaacctcc gcgagataga ggagcagggc
      841 ctcgatgtat ggctgcctgg agaagaagag aggtggcgct gcccgacgtg cgggaaaacg
      901 atcgtcttct accactaccg gtgcccggca tgcgggcatg agccggaccg gaaagcgtga
      961 aggcggcacg ttccaggagg acggttgccg cctccccctt cgaccagccg ccggtccacg
     1021 acggcgtgcc cggagggaga gaacggccgg gcgaggaatg gggtatcgcc tccagcacaa
     1081 caatgatatc agggatgtga tcgaaccttt ctgcagaacc gtccgatagg acaacaaggg
     1141 agcctaaacc gatgaaaaca gtaaaactat tacagatagg cgatatgcag aacggcttca
     1201 tacgggaaga tggcaatctc tatatcaggg gcgcacggga gatcatcgca cccgtgaacg
     1261 actttctgcg ccgggtgaga aacggcgtct ttgatcacac gttaatcaca ctcgatacgc
     1321 attttgcgga agaatacagc aagactgaag aaggtaaggt ctttcccatc cactgcgaat
     1381 acggcacaaa cgaccgggag ttaggtatcg acgtctccgg tctgccggat aagcggtatt
     1441 tagcgaaaaa tcaatacgac atgtggagcg agaggacgtt aacagacgta tcgttcaacg
     1501 atcccgggcg aaagatcgcg cacgacaacc tgtttcatat cgtagacgat ccctatcagc
     1561 cgacggagag agtaacccgc gacgatttca tcgaagcgat cagtcccggc ggcaacccca
     1621 ccggcattga agtcaccctg atcggcgtgg cctcggatta ctgcaaccgc tatgccatgg
     1681 aaggctggct cgagcggggc gccagcgtaa ccatcattcg ggacctgacg aaaggcatcg
     1741 agaaagagac gcctcaggtg ctcgacgaac ctcgataccg gcagtataac ggcagcaggt
     1801 tacgatccgt cgagagcacg gaatactgaa ctggctgaca ccacactatc gtcctgaagc
     1861 accaccagag atccgtaatc cccggcgata catagcgttc atgcccccgt tttgccgccg
     1921 tatcccgcca gccttctaaa aagaggatat tttctcgtcc cccacggctc cccggcctta
     1981 cgcagtttcc gccgcggagg ggagaccgga actcgccgtc ccgctctcgt agacgagggt
     2041 gttgtagcgg tactgaaaga tgtagaccac cagagatacc gcgagcataa ggaaggggag
     2101 gaggagaatc ccgataatgc ccgcgacgag tccgatgagg cctgttacga ggaagacgag
     2161 agagaccagg accatcatga agagcataag aacgatctgg aggatgacct gggagaagat
     2221 gtacgatccc cagccgatcc tctggatctg agcgaggacg gcggggatgt tgaacgcttc
     2281 ccgtaacgac tccatcttcg cgaaccgcac cgccgccatc tgcgccacaa gccagatgag
     2341 gatgaggaga acgaacgata cgagagcgac gactgcggcc acaaggccgt ttgtccccgc
     2401 caggataccg gcgcctccca tcagggccat cgcgacgagg accatgagcg cgacatagac
     2461 gaggtacacc acgagcagct tcaggccgtc gacgaagagc ctgccccagc cgccgaggtc
     2521 cggcgtgtcc ccccgcatga tccggacgaa gtagccgtag atgagcggaa agatgacaac
     2581 gcagacgatg agctggagcc accgtatcca gctcccccgg agggcatccc ttgtgtaacc
     2641 gaacgaatct tcgagtacgc tctggtagtt catatacgac gggcaaggag a
//
LOCUS       DAJB01000030           14255 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_89023, whole genome shotgun sequence.
ACCESSION   DAJB01000030 DAJB01000000
VERSION     DAJB01000030.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14255)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14255)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14255
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_89023"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2171..2220
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9101..9118
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2590 a         4428 c         4381 g         2788 t
ORIGIN      
        1 cctcttcctc ggaatcgagg atcgtgaacc cgataacgcg atcgccgtcg tcgagccgca
       61 tgatacggac accgcgggtg ccccgcttct ggatcgagat ttcggaaact ttcgtccgga
      121 tcacgatgcc ggacgcgctc atcacgatga tctcgtcgtc gtcggagact gctctcgacc
      181 cgacgacacc gccgccccgg gcatcgacga cgatgtttcg caccccgagc gtacccctgc
      241 cgtggccgcg gaactcgtcg aactccgtcc gcttcccgaa gccctgctcc gtgatggtga
      301 ggagatggtc ctcctcgacc acggatatcg cctgcagggc atcgccgtgg cggagtttga
      361 tcccccgcac gcccatggcg ttgcggtgga ccgggcgtac ggcctcctcg tggaaccgga
      421 ggctctgccc gaacttcgtc gtcaggatca gctcctgatc gccgtcggtc gccttcacgt
      481 cgaccagcgc atcgccttcg cggaggttga tggcgttgat ccccgtctgc cgtggccgcg
      541 agaactcgtc gagcgccatc ttcgcaaccg ttcccccgct cgtcgcaaag aagaggtagt
      601 ggtcggcccg gaactcccgg accgggatca ccgcggtcac ccgctcctct ccttcgaggt
      661 tcaagaggtt gacgagggcc ttgcccttgc ccgtccgctg tccctcgggg aggtcgtaga
      721 ccttcagcca gtagaccctc ccgaggtcgg tgaagcagag gaggttgtcg tgcatattcg
      781 cgacgaagac gctctcgacg ccgtcgtcgt cctttgtggc cataccgatg accccgcgac
      841 ctccgcgccg ctggttccgg taggtgtcga ggtcgattct cttgatgtag ttagaggagg
      901 tgagcgagac cagcatcggt ttatcctcga tgaggtcctc ccgatcgaaa gcctcggccg
      961 tgtgcccgat ctgcgtcctt cgcgggtcgc cgaagcgggc ggccatatcg acgagttcct
     1021 ttttgatctc ggcgaggatg cttacctcgc ttgcgaggat cgcggagagc cgctcgactt
     1081 cctttgcgag ggtatcgcgc tcgtcctgga tcttctggtg ctcgagcgcc gcaagccgcc
     1141 ggagctgcat ctttaagatc gcatccacct gcacctcgtc cagcccgaac ttcgcgacca
     1201 gggctgcccc ggcctcatcg gtcgtcccgg atgcccggat ggttgctatg acggcgtcga
     1261 tgttgttgag cgcgaccagg aggccgctga ggatgtgcct ccgctcctcc gtcttcgcga
     1321 gatcgaactc ggtccgccgc cggaccacgt ccacccggtg cttgaggaac acatggacaa
     1381 tttccttgag gtttaaggtg cggggctggc ggtcgacgat cgcgaggttg ttgatgccga
     1441 aggaggactc aagcgccgtg tgcttgtaga ggaagttcaa tatcacctgg ggcgcggcac
     1501 ctctctttaa gtcgatcacg acccgcatgc cgtcccggtc cgactcgtcg cggatgtcgg
     1561 cgatcccctc gaccctcttg tcacggacga ggcttgcgat gttctcgatc aggcgcgcct
     1621 tgttcacctg gaaggggatc tcggagatga cgatctgcgg tgtccggcct tcttcctgga
     1681 tctccacgac tcccctgacc acgcacttcc cgcgtccggt caggtaggcg tcccggaccc
     1741 ctgcggtgcc catcatgatc ccgccggtcg ggaagtccgg gccgggcatg atctgcatca
     1801 gctcttccgt ggatacgccc ggttcgtcga tgatccggca ggtcgcctcg cagacctccc
     1861 ggaggttgtg gggcggcatg ttcgtcgcca tgcctaccgc aatcccgctc gatccgttga
     1921 tgaggaggtt cgggacccgt gccgggagga cgctgggctc ctggagcgac tcatcgaagt
     1981 tggggacgaa gtcgacggtc tccttgtcga tgtcggtcag gagttcctcc gagatcttcg
     2041 tgagccttgc ctccgtgtaa cgcatggcgg cagcagagtc cccgtcgacc gacccgaagt
     2101 tcccctgacc gtccaccagc atgtggcggt aggagaaggg ctgcgccatc ttcaccagcg
     2161 tatcgtagat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 tggaccggct ttaagccgtc tctcgcgtca gggatggcgc ggccgatgat cacgctcatc
     2281 gcgtagtcga tgtagcatga tttcatctcc tcttcgatgt tgatcgggac aacctggtca
     2341 gatgtcaagg ttgttcacct cctttgcgtg ccggcggatg aagtccttcc gggcatctac
     2401 attctcaccc ataagtttct cgaatatatc gttcgcgtag atcgcgtcct cgatcttgac
     2461 ctgtttgagg atccggttcg ccgggtccat cgtggtgctc cagagctggc cggcgttcat
     2521 ctcaccaaga cccttgtagc gctggatcga gacgcccttc tcgccccact cggcggtgat
     2581 ctcccgcatc tcctcctcgc ggtagacata ccgctcctgt ttgccccggg cgacccggaa
     2641 gagaggcggc tgggcgatat agatgtaacc gtgctcgacg agccccgtca tgtagcggta
     2701 gaagaacgtg agaaggagcg tccggatatg cgcaccgtcg acatccgcat cggtcatcag
     2761 gatgatccgg tggtagcggg cccgctccgg gtcgaaactg tcgccaatcc cggtgccgat
     2821 agccgatatc aacgcctgaa tctcggcgtt cttcaggctc ttgtgctccg acgccttctc
     2881 gacgtttaag atctttcccc gcaggggaag gatcgcctgg aactttctgt cccgcccctg
     2941 tttcgcggaa ccgcctgcag agtctccttc cacgatatag atctcgctct tcgccgggtc
     3001 ccgctcctgg caatcggcga gtttcccggg gaggccggtc gactccagcg tgctcttccg
     3061 ccgggcgagc tcgcgtgcgt tcctggcggc ttcccgggcc cgggcagcgg tcatcgcctt
     3121 atccacaatt acctgaaggg ttttggggtg ctcttcgaag tactcggtga gcgacgagta
     3181 cacaagcgag tccacgatgc cccggacgtt gctgttcccg aggcgcatct tggtctgccc
     3241 ctcaaactgc gggttggcca cccgggtgct gatgacggag gcgagtccct ccctgacatc
     3301 ttcgcccttg acctgggcgt cgttgttctt gaggaggttg tttctgtggg cggcgctgtt
     3361 gatcgcccgg gtgagggcac tccggaaccc ctcgaggtgg gtgccgcctt cccgggtgtt
     3421 cacgctgttg acgtaggtgt agattgtctc tgcgtatgag tcgttgtact gcagggccac
     3481 ctcgacctcg accttgctct cggggtcgcg cttctggaag tagatgatat cgtcgtggag
     3541 gctctctttc ccctcgccga tgtgggcgac gaactgcctg atgccgtcct cgaaacggtg
     3601 ggtgaccgag tccccggtgc gttcgtcccg gaggctgatc gtaaggccgc tgtttaagta
     3661 ggcaagctcg cggagccggt ggtccagcac atcgtagtcg aactcgacgg tctcgaatat
     3721 cgaccggtcg ggctggaacg tgatccgggt cccccggagc cgctcgtagt cgagcggctg
     3781 cccgtcccgg gcaccgtagc gctcctcgta gcgcatctcc atctcatgct cggtctccgg
     3841 gtggcaggtg agcggctttg caaccaggcc ctgccggaac tgcatcgcat agacgctgcc
     3901 gtcccggtag accgtcgcgt cgagccagct cgagagagcg ttgacgaccg agacgccgac
     3961 accgtgcagg ccgccggata cctggtaggt gttcttatcg aactttccgc cggcgtgcag
     4021 caccgtgaga acgatctcaa gagcgctctt gccgtactgg ggcatgaggt cgaccgggat
     4081 gccacggccg ttatcgtcaa ccgagaccga gccgtcccgg ttgacgacca cctggatcag
     4141 atcgcagaac ccggcgagag cctcgtctac ggcgttgtcc acgacctcgt agaccaggtg
     4201 gtgcagtccc cgggtctccg tgctgccgat gtacatggcg gggcgttccc gcacaggcct
     4261 taagccttcc agtaccgtga tgtgggaggc atcgtaggta tcagtcatgt gtgacctccg
     4321 ctgaacgagc ggcgtcctga atcacagtaa aactcacaat atatatgggt tttataacca
     4381 cttatactgt atggctctga gaaattcatt gttttagggc tatcctgtct gtccacggtt
     4441 tgatacggtc tccccgggag cgcgcccgaa aaggtcgggg atcacaccgg ataagcagtc
     4501 tccggacgcc ctcccctcag tcgtatttcc cggtatcggc gtcccgtatg ccaagctcgc
     4561 tatcgactgc gaccgcgatc cggttgagga gtctcttgat gtccgccgca tcctccttgt
     4621 ccagggtgcc cggctggtgc cagatcacca tcttgcggag acgctcgatc agctcctcga
     4681 tctcggttcc ccggaactgc tccggcaccg taaggtcgtt gaggtgatcg gcggcgccga
     4741 agaaggcttt ctcggggggc aggccgaagt gccgtgaaag cagcatcagg ttgacgacga
     4801 accctctgcc gaactcgctc tttgcgggca taattaccct atgcaccgcc gactgatatg
     4861 aacgatccgt ttgcggttcc cacccggaga ccggaggctt tcggcgtgga ggcatgcccc
     4921 tcacggcaaa aattggatct cgtactcatc aagggcgcag gtagatcagg accgtggaga
     4981 gcacgatgaa ggcaagcgtc gcgacaaaga agatgctctc tccggcatgg agagtttttc
     5041 tcccgatgta caggctggcg ttcgccccgt agccccggca gagcatgctc gtgtaggtgc
     5101 gctcgccctg ctcgtaggac cggatgaaga tcgtcccgac cgtgtagccg atctggcgga
     5161 gccggtagcg gtaggggagg gtgcggtcga gagggtcaaa gcaccgggtc tccatcgcgg
     5221 tccgtatcct gccgaacata tcggcaaaga cgaagatgta gcggaccatc atcccgagcg
     5281 tgagggtgaa ctcccggggg aggccgagcc tccccgccgc ctcgatcatg tcctgcatcc
     5341 tcgtcgtcga tgagagcagg atgatgaacg atatgctgac gacgaacttc acaaggagga
     5401 ttgacgcaaa ctgcaccgat tctgcgtaga cctcgatccc gaagggcagg gtcgcaatcg
     5461 tgtggaagac atcgtagtat gggttcttca cgaatacctg gaatccgatc aggaagatcc
     5521 cgaacggcag gatgagcgcg agcctccgga ggtagaccgt cggcgggagg cgcgatgcga
     5581 cccagaggac gacaacgaga gcagcgagga cggcgccgag ctcgtagacc ttcgtcgagt
     5641 acggcagggc tacgatggcc acgatggcag cgagggctat gaggagtttg atccttgcgt
     5701 ccagccggtg gacgacgctc tctctgtaag catacttctc gatgaagtag aggtcgtcga
     5761 tcattcttgt ctcgtgtagg cgtcgaggaa cgacttcttc gccccctcgt aggtgtaggc
     5821 catatcgatg gcgacaccgc tctcctgcag cgaccggatc agtttcggta cgatcggcac
     5881 gtcgagccgg gtccgggcga gcagttccgg ccgggtgaag atctcctcga ccgtcccgga
     5941 ggccacgatg catccgtgat ccatcacgta gatgaagtcc gccacctccg ccacgaggtc
     6001 gacctggtgg gtcgagaaga tcaccgtcat gccgtactcc tcgggcagcc ggttgacgaa
     6061 cgccacgagg tcggcgacgc cctggggatc gagcccagca gtcggctcat cgagcaccag
     6121 cacctgcggc tccatcgcga gaatgccggc aatggcgacc cgtttcttct cgccgcccga
     6181 gaggtggtgc ggcacgcgct cgcgcaggtc ctcgatcccg aggaggtgca gggcctcctc
     6241 gacacgatgg gcgaccgtcg ccgcgtcgag gccgaggttg gtcggaccga acgcaacgtc
     6301 ctgctccacg gtgggcgaga agatctggtc gtcggagttc tggaagacga tcccgacgaa
     6361 cttgcggatc tcgcggacgt tctccttcgt gataggctcg cccctgacca gcacctcgcc
     6421 ggacgtgggc ctgagtatgc cgttgaagtg cttgaagagg gtactcttcc cggcaccgtt
     6481 cggcccgatg acggcgatgc gagacttcct ctttgcgatg aagttcacgc cctgcagggc
     6541 gggaacattg ccacggtaga tgtgagtgag agcgcgggtc tcgaccaggt gcatagtagc
     6601 aaaaagtggg agttttttgg gtatctaatt atcgggccgt gcggagattt tcgcaacgcc
     6661 gaataccagt acgagaataa gaagcgtacc gagcacgatc gcaagcacct ctccggtctt
     6721 gtccaggccg gggatgctgt agtcgggcat gaacgcctcg tatgagaagt cgttcccggt
     6781 cgtggcgatt gcctgctcct ccgcggcgcc ggcagcctct tcattaagtt cgctccccat
     6841 gaagggcttt gcgtcgtgga tgctgaagaa cgcgctctcg aggccgtcgg ggtttccgga
     6901 cgcgaggaat ggcgcggcaa cggcgatcac gagagcgatc gccacgccga tgatgacaaa
     6961 ctgtttcgtc tccattacgc gctcacccct ctcgggtgct cgaggatgtc gggccgtgcc
     7021 gatgcgatca ggtagagagc gaccgcagtg atacctccct cgatgaaacc gatgacggcg
     7081 tggtatcccc ccatgaaggt gagcccaata acgagcggga atgtgccagc gattgcgagt
     7141 tcgaccgcac agaggatcgc ggagatgaag agcgacgccc acccggcgat gaacgcggcg
     7201 gcatacgggt tccctatcac gcccttgatg gcggtgtagc cgtagtagcc gacgaatccg
     7261 ccgatgacac ccatattgat gatatttgcg cccatgacgg tgatgccgcc gtcaccgaag
     7321 atgacaccct gcacgaggag caccagcgtc aggacaaaga cccctgcaaa cggcgatccg
     7381 aggacgattg ccgcaagtgc cgcaccgacc atgtggccgc tcgttcccca gggaatgggg
     7441 atgttcaatg cctggatggc gaagatgccg gcggcgagca cggcgaccag cggtatcttc
     7501 tcttctccca tcgagcgccg cgcccaccgg agggcgagag caatgaagat gagggcgatg
     7561 acccagtaga cgagggcctg cccaatcgga ataaatgcat caggtatatg cataaaacac
     7621 ccgaagaatt gtattacgtt taggtattaa tgtattacta agcgcgaggg ttgtatcaac
     7681 ccgtaagaat ctgacgtact cctgatgcga aaagtatgcc cattgccctg accaacgcgg
     7741 gtttcagggc aagtttgcgg atcagggtgc ccggccggtc catatcgccg tgctctacga
     7801 tcgtctcgat gaggtcgggg tcgtttaagg cccggcagag gcgatcgatc tcctcgggcg
     7861 tcatcctctg ccgcattctg agcgctttca taccgatatc gagttctttc ccgaagtctt
     7921 ccttccagcg ccgttcgtag tcccggaggc tcccgtcatc gaacgtgccg cgcgccaggc
     7981 aggccgccgc gacctcagcg gcgtgttttg ccgaccggat gccggtgtag accccgcccc
     8041 ccgacgtggg tttggcaaac cctgccgcgt cgccgacgaa gagcgcccgg tggccgtagg
     8101 tctgcggcat aatcccgagc gggatgaccc cgctcgcgag gtggagcgag ttccctccaa
     8161 agcgaccgat gaaccggtcg aagtggtcct tcgcgtgctc ccgtgcgcag agcccgacac
     8221 gggcccgggt ccccgagacc gggatcaccc acccgaagaa gtcgggcgag gcgttggggt
     8281 gcagttccac gtagcgcggg tccagtgcat agggcacctc cgcctgtatc ccggcaaggt
     8341 agatctccga acgccggagg ccgagcatgc gggcgacgga actccgcggc ccgtccgcag
     8401 cgatgacgag gcggaacggg atctcctcgc gcccgcggac accccgggtc tggagagagg
     8461 aaccctggat gcccgatacg ctggtcttta cgaggaactc cgccccggca tccgccgctc
     8521 tccgtgccat ctcgcggtcg aggaggcccc ggtccacgac atacgccttg gtgacccgtg
     8581 catcgaagag gagttctccg ccgagacccg agaccatgcg ggcgccgctc acctcgttct
     8641 ctaccgatcg ctctgagacg ccgcactcgg caaacgcgga cgcggagagc agtcctgcgc
     8701 actgcacggg gtagccgatc gtcccgtgct cctcgataca gagcgtcgaa agccccctct
     8761 ccgcacacgc ccgcgccgcg gcactcccgg cggggcccgc gcccacaaca acaacatccc
     8821 agaccagatt accatgcccc gcacacgtgc atttccggtg ccggttgcct gccccgtgct
     8881 tgcaggccgg cacccggttc ctgagtagag tggacgtctg gcaacataaa agggagcggg
     8941 tgggaggagc gagggtttgg ggtgactgcg cacctcgcac ctccggtgct cgagctcctg
     9001 ccctccggcc cgccccctat tacacttggc agtgcttctg ctctggttct cccaaaccat
     9061 cgcaagtcca agaccttcgg tcttctcctg ctcctgccct nnnnnnnnnn nnnnnnnngg
     9121 gggatgttcc cccctccccg tcagcccctc ccccaatcgc gatatccact ggaaatccgt
     9181 gtgtgggttt atagcgtcct gaggaacggc gggcgcgata tccaaatccc ccaaaccccc
     9241 tccccgcccc cgcgcccccg tccccatagc tgcgtatttg acggatcatc ccccaactag
     9301 tacgaaatgc ctgatatacg ggagttcaag tggaagcaat gcctggtcgt gcgcgccgac
     9361 gtcaagatga gttgcggcaa gaagtgcgca cagatcgccc acgccgccat cggggcctac
     9421 gagaaggccg acaagatcgc gcggaaggcc tggcttgacg agggacagaa gaaggtggtg
     9481 ctgaaggcat cgaccgagcg gcagctcttt gaactcaaga cgattgcaga gcgggcgggc
     9541 atccccgcat cgatcatcca ggacgccggg atgaccgaga tcccgccggg aaccgtgacc
     9601 gcgctcggtc tcgggcccgc ccgtgccgaa gacctcgacc ggatcaccgg cgacctctcc
     9661 ctgctatgat gccctcgccc tatcccctcg aagaggaact ggggatgcgc tactacgcct
     9721 ccgatgcccc cggcatcggc gggcggctcc gctcaagtca cgaggacttc gtcgtcgaag
     9781 agctgccgct cccgatcagc gatcccgatg ggccgtacct gatctgccgg ctcaccaaga
     9841 cgaactggga actccagcgg gcggtcaagg agattgcaaa gcggctcggc atcagccacc
     9901 ggcggatcgc ctgggcgggg accaaggaca agaacgcggt gaccacccag ttcatatcga
     9961 tctacgacgt ctcgcccgaa gcggtcgggc aggtgcatct tgcggatatc acgctcgagg
    10021 tcgtcgggcg gtcgcagcac tcgctcgtcc tcgggggcct cgcaggcaac cggttcgata
    10081 tcaccatccg ggactgcatc gcagaggatc tcccggcgcg ggtgcaggcg gcgacggagg
    10141 tcgcgtccgt cgggataccg aactactacg ggctgcagcg gttcggcgtc gtccggccgg
    10201 tcacccacgt cgtcggcgag agcatcctca agggcgatta cgagggcgcc gtggccacgt
    10261 atgtcggccg gtcatacccc cgggaatcgg aggttgcgca acaggcccgg actcacttcg
    10321 caaagacccg ggatgcacgg gcggggctcg ccgccctccc cgtccagatg acctacgagc
    10381 gggcgatgtt gaaccacctc gttgcgaacc ccggcgacta tgccggtgcg cttcaggcgc
    10441 tccccccgaa gctcctctcg cttctcgtga gcgcgttcca gtcgtatctc ttcaacagcg
    10501 cgctctcctc ccgtatagaa gccggccttt cgctgaccga gcccgaggtc ggcgaccggc
    10561 tgctctttgc aaacggccgt gaagacatcg tctccgcgcg gaaccgccag gcggccctgc
    10621 tgcagatccg tcgcggccgg tgccggatcg ctctcttcat cccgggctcg gaaccgatag
    10681 tgtcgcacgg gccgatggat gagatcctgc aggaactcct gcagaaatcg gggatcgaca
    10741 ccgggaattt cgagcgcgcc tcccggttcg tctcgacaaa gttcgccggg gtcctccggc
    10801 cgatcagcct caccaccgac gtggaggcgg atgtggtcga tgcgagcgtc aggctcaggt
    10861 ttacgctccc tcccggccac tacgcgacga cggtctgtcg ggagtacatg aaggcggacc
    10921 cctatatgat gatctagccc aaaaattaat ttccgggagc gggactgtag agcacgtccc
    10981 actccgtgac caggttgctc ccgcacatga agtggcgttt caaggagagc cggaacatct
    11041 cttcttcggt ctcgatatgc atgcccccga cgagggacgg ggtatcggca ccgccgacga
    11101 taaacggcag gtggatcagg cgtatctcat ccacgagacg gtggtttaac atggaccagt
    11161 tcagcgtcgg tccaccctcg atcatcatcc ggcagacgcc gtaatctctt ttgaggaccc
    11221 gcatcagttc cggcagatcg acctggctcg ctcccgcgac aacgacatcg acgccgcctg
    11281 cccggagccg cgcaaccttc tccggcggtg cagcctcgct gacggcgatg accgtccggg
    11341 cgtccgaccc gaggacgttt gaatccggcg ggatatcccc ccggctgttc ggaatgacac
    11401 ggagcgggct tttcccctct accagccgga ccgtcaggaa ggaattgtcg atcctgatgg
    11461 tgtttgctcc gaccatgatg gcgtcgtatt ccgcccgggt ctggtgaagg aggagttcgg
    11521 tttcgggggc catatacttc atgagaatct tgctggaggc cccgcgcttc agggtaagct
    11581 tcccgtcgac ggtgatctcc gacatcatga gcacgtgcgg cctatcgtga tctggcattg
    11641 gtcttcgttc ccggtgatct atctggtggg agcgtttatg aatctggtga ttctggagcg
    11701 tatgcactct tccaggtgag gtgttgcggc cccgtccgcc agagcaaagg ttaaacccct
    11761 tccgggctga taaattatgc gatgaatata acctctctgc ggccgaaatt catcaaacat
    11821 acggagccaa tagcgtcgtt tttcatacgc ctgggcgtta cgccgaacca ggtctcggca
    11881 ctctccgtgc tcttcggctt ttcgtgcgca cttgcctttg cccagcgcta cttcctcgcg
    11941 ggcagcctgc tcctggtcgt ctcggcgatc ctcgacctgg tggacggcaa tgtcgcccgg
    12001 aagaaccata ccgagagcaa gttcggggcc gtcttcgact gggtcgccga caaatacgtc
    12061 gatgcggcga tcatcctcgg cgtcgggttc tccggcatcc ctatcgtcag ccacctcata
    12121 aacgtcccgc ccatcgccga tttcggcgtc gtgggactgg cgctcgccgg atccctgatc
    12181 aataccttca tcaagccggt cacgtatgcg gagatcggct attccgagag gatcgccggg
    12241 aagatcgagg atcccctcga gggagtcggc ttcttcggca ggcccgagac gatcctcgcg
    12301 ctggtgctcg gcggcgtgac cggctacctc tgggttgcgg tgctcctcat cgcggtctgc
    12361 acgaacctct ctgcggttca gcggatcttc tacctctacc ggcagtactc ctgaacggag
    12421 accgtccttc catttttcat tctttggccg tgcagcggca gcgtagccct tgcgggaaaa
    12481 gagcatgccg ggatcgtcgg ccgggccgga tccggcaggc gctcgcgctt tgaggccatg
    12541 cgggtggtcg gggcgggccg ccctgcgggg atcgcaggac ccgatcggga tcctcgccag
    12601 aaggggttca gggcccggct ttcggccgga caatccgctc cacctcgccg cggtagagcc
    12661 ggtagaggct gtcggcgagg ttacccagtt cggggaggat catgaagaga gcataggcga
    12721 ggaccacgag gaatacgagt gctccgagtt cggccataac gttgtgcgcc cagaagaaac
    12781 tctcgtaccc gagctcgccg ttcccggcga tcgtggagag gtaagggaac cactgctcgg
    12841 tgtacgccgt gatcacgaag acgtttctga cgatgtttaa gatatagatc gtcgggaaga
    12901 ccaggaggag gccggcgatc ttctgccgcg tcgtcgactg cacgccccag gcaaccccga
    12961 gcatgatcgc gatactctgg atgccggtgc aggcaaggat gatctcggtc tggaacccgt
    13021 tgcgctccat caggttccag gcactgaagt gcacgggata cccgactgcc gcgagtgccg
    13081 cggcggtctg atctgcgact gctgcaatca gccagtcccc gagtgcgggt atgtagccga
    13141 agggcgcaaa gatcaggaac gccacggccg ctatagtcga gagctgcatc acccggggat
    13201 cgtcgcggag caggtacttt gcggtgatat agaggaaagg aaccgagagc acggctatcg
    13261 cggggtacat gaaattgttg agggagaagt agtacgggag ctcgagaaag agatatccga
    13321 cgatgcttgc ccacccgccg atggcaaagt acttccggaa ccgcccgggg ataaggaaga
    13381 cgagaaagca gatgcaggag atcagcacca gataatcctt catcctttac ctttcggcgt
    13441 atctgacatt aaagcatttg tgcgcccgat ccgaccgagt ctccggtggt gcagtcccgg
    13501 ccacccgact gaccaagggt taattttgat cggcacacca tttattataa tgatgttctg
    13561 tccgcagtgc aaaggcctga tgatatcgtc tggtggtcag atgaaatgca aaaaatgtgg
    13621 ctatatcagg gagatcaacg acctcgaccg gttgaagaag acagacaagc gcgtggagaa
    13681 ggagatcacc atcgtcgacg aagaggacac gattgcaacc ctcccgaccg cgaccgtcaa
    13741 atgctcggag tgcgaatgca caacggcata ctggtggctg cgacagttgc gggcagcaga
    13801 tgagagcgaa gtcaggttct accgttgcac cgcctgcggc aagacgtggc gcgagtacga
    13861 ttaagcagat ccgtatcctc cgttgcccct ccggggcctc ttcttccttc tgattgggct
    13921 tgtaggtcta tcggccggta tccttcattc gccccgccgc gtccacgctc tctgttcctg
    13981 tgagggcatt tccgggtggt caactctgca gatgcgagcg cccccacaac cagggggggt
    14041 gcagggtatc ttgtatgctc tctgccggaa tgccctgttt cgtggtgaag cgtgccgtgc
    14101 gaaaggctga ggcagtccgt cagaagacag gcaacccgat tgaatgaagt gccgcaccca
    14161 atgtccgctc atatacgacg ggcaaggagg cccccggttt caaccggggg aggaattgcc
    14221 cgcatcaggc cccgtacacg ttcgccccgc gggag
//
LOCUS       DAJB01000031            3575 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_28379, whole genome shotgun sequence.
ACCESSION   DAJB01000031 DAJB01000000
VERSION     DAJB01000031.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_28379"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    863..883
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          827 a          927 c         1057 g          743 t
ORIGIN      
        1 gatgatcagg ttgtcgcggg agtcgatgcc gatgtgctcg ccccggacgg cgttcccgtc
       61 gccgttcttt ttgaggcagc cgtagagggc gtcgaagttc ccgacatcgc tccactcggc
      121 ctcgaaggga acgacggcag cccgccgggt cttctccatg atcccgtagt cgatggagat
      181 cttcggcgtc tgctcgtagg cctgctcggc cgggttctcg aacgcccgaa caacgtcagg
      241 ggcaagggac gcgcactccg caaggaagag atcggagtcg aagaggaaca tcccggagtt
      301 ccagaggtag ccgtccgcga cgtaccgctc cgcggtcggg aggtccggct tctccacgaa
      361 ggcatcgacg gcatacccgt catcgagcgc ctccccgggc ctgatgtagc cgtagccggt
      421 gtggggcgag gtcggccgca ccccaaagac caccaggcgg tcccgggaga ggcgctccgc
      481 cttccggaag gcttcgatgt accggtcgtc cgcgtcgatc aggtggtcgg aggagaggac
      541 ggcgaccggg gaggagccgt tcccctccgc aatcgccttc accccgtagt agatggcggg
      601 gagagtgttc tttccctccg gctcgaccag cacggcgcag tcgcacccga tctcggcgag
      661 ctggtcgcgg atgatgaact tgtggtcctt gttggtgacg atgtagatgt ccttcggagt
      721 ggagaggagg agagaccgct tgatcgtctt ctggaagagc gattcctcgt cgaagagctt
      781 gatgaactgt tttgggtagt gctcgcgtga gagcgggaag aggcgggtgc cgcttcctcc
      841 cgcgagaatg agtgtcttca tcnnnnnnnn nnnnnnnnnn nnnattagcg aagtactgat
      901 accggccagc actactccta cgagatggac agaatcttcc tcgaagagct gaactcccgc
      961 agccggaata caaccccgat gtcggttccg ggctgcacgg cgagcagaca gaacaatcgc
     1021 atcaaccccg gtaccaggag aactaaaaaa ctataagaac gctggaacta tggtatgtac
     1081 tggaaggcgg ttttgattca gaaaataaca gaagaagaga tccttgcgac gggaatcagg
     1141 tcggttgcgg aggatcttcg ttccagggac ggtgtccttg cgcttatcct cttcggatcg
     1201 gcagcacgga accagcaaag accattttcc gatatcgacc tctgtatcgt cacgtccggg
     1261 atggttcccg agaaagagca cctggacctg aaaagttatg gttcccacac gatcgagatc
     1321 catctccttg acgagctgcc gcttgctatc cgcttcaggg tcgttagtga gggaaaactc
     1381 gtattttgca aagatgccct tgcattgcac agagtcgtcg cagataccgt caggcaatac
     1441 ctcgatggtg catggttcat agacctgcac agccgtcatg cgataggaat ttcccggtga
     1501 aaagatgacc ggagacgtcc agtacgatat cgagcggatt ggatcgatct gttcggatat
     1561 caggaggtat ttggtggacc ttgaacaact caatatcggg tacgccggag atctttcaga
     1621 caagcgcaat ttttacgcgg tctccatgat tctcttttca ctcctgaatc gggtcctcga
     1681 tctgggcaac gaggtgacga tcgcacataa tctcggtatc ccaaagagct atcgcgatat
     1741 attcatccta ctctggaaga acggtttcat cgatgaaaaa tgtgcaaaag agatgggcag
     1801 gctggtaacc taccgtaacc tgctgtcgca tgaataccag gggatcaccc cagaaaaggt
     1861 attcgaactc acgaaaaaag tcaacacaat aaaaaagttt gcacggttca tgcaggaaaa
     1921 agtcaaggag caatgaggcg gtctttggag catctttcac caaaaatctc tggtaatctc
     1981 tggcacattt tgacattcca gaatattgat attttcatcg gcaaggaact tcatccggca
     2041 atcttttcct ggttggatgt ggaatatacc cgctcacact gaacaatctt ttgtgcatac
     2101 cgatacaggc taagatatcc tcgtgttcaa gcccgggctc ctcgcccttg agcggcgact
     2161 cccccggttg aatctttacc ccgacgaagg ggagtttcgc tggaagccgt agacgtgctc
     2221 gatacccggc acatccgcaa agagcgccgc cgccgggatg ccgacgtagc ccatgccgaa
     2281 gacgccgacc ttccggatcg ggccgcgttt ctggataagt gctgcgaggt tctcggtcat
     2341 tgtctgcatc tcttgtttgt cttcaagatt gatattctgt cttctgccac ccccttttgt
     2401 agtgcaggca agggtccgga gagagtttcg ccgggtgaag tgcagatctg gagaaactat
     2461 ttatgcccac catacagagt gaaattgaat gaaacgattt gtggccattg cacgggggcg
     2521 tgtccagcgg gtcggctatc gggagcatgt gtacaacgag acgttcgaac ggaatatctc
     2581 ggggtacgtg aagaatctcg ataccgggga ggtcgagatc gttgcagagg ggagcgagca
     2641 ggatctccgg gatttcatca acgagatcaa tatcatacga agacctattg cagtcaaatc
     2701 gttcaccgtc agatgggaag aagccacagg agagtatgcc gattttgaga tcatccgggg
     2761 agatagccag gaggagacgt tcgagcggat ggattacgcc gggaatgtat tgcacagcat
     2821 ggatatgaaa ttagatcagg gcctccagtt acagcacacg atgctggaca agcaggacca
     2881 gagcctccag atcggcaaag agacgaaaga agagattgca gggctccgaa gcgataccag
     2941 aaagcacctt gaggacgaat tcgcagagat gagaaaggag ctcgcctcca tcaaggatgc
     3001 gcttgcccgg gcaggcatcc aggtctgaat aactctccat cccgcgtcaa ctaccccggg
     3061 ccatgcgtgc ggtcgtccca ggggcgagac tcctggctga ccgggacggt gacccgcttg
     3121 atcctgtagg cccgcccctc tcctgcggcg ggaaggagag gatctgccgg taagcgggaa
     3181 agaatgatat gcaatgcggg aagagatacg ataatatgag acaacgccac ctgctggatg
     3241 cggaagagaa agaagaggtt ctccgcacca tccgcacggt cctcgccgga tttgacgaga
     3301 tcgaggtggg gtatgtcttc ggaacgtttt gcagggggga ttttggggac gtggacgtgg
     3361 caatcctcgt caccggcgaa cccgctccct accaggcgat gcggtttcgc gcgagggtcg
     3421 agcgggagct tgaacgaggg ttctgttacc gttttgaggc tgacgtgaag atcctcaaca
     3481 ccgccccggt ctctttccag tacgaggtca tcaagagcgg ccgacccgta ttcctgcgcg
     3541 acagagagca gaggatccgg tacgaggcgg gcgtg
//
LOCUS       DAJB01000032           10331 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_31182, whole genome shotgun sequence.
ACCESSION   DAJB01000032 DAJB01000000
VERSION     DAJB01000032.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_31182"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1715 a         3322 c         3370 g         1924 t
ORIGIN      
        1 cggcgacaat accgagatgg ccgatggaga ggatcgaggt gtcgtcgaaa ccgtcaacga
       61 gaggcatatt gaaggttatt cactgaaagt acaaggatct tgcggttttg gatattcaga
      121 gggggtggtt cttactgcgg atgatcggca gatcgtcgtc gacccgctca acgtggacgt
      181 acaggccttc gtagacaccg ttgatgtaca ggtcgaagta cttcgttctc ggtgccggct
      241 gcccgagaac gtggaagagg tccatcgaca gcttctcccg catcatcgtg ggatcggtgt
      301 acgtggcttt caggttcatg gcgttgctgc cgaacagaaa ctcctgagat tcgttaaacc
      361 ggatgtcgaa tgattttttc ggcaactccc gcgaagaagt gccccggaag cgcactcctt
      421 ccagttcgat atcctcgccg tcgggcgaga ggcgtacgga gccacccacc cattcgtcgg
      481 atgcgtctcc ccgggtgtac agctcgacga gatccttcca ggccatgaag agatacaggt
      541 cctgcacgcc ggaatccggg ccgctggcgt tctcagaccc cgccgctgcg ggtatgaaaa
      601 aggctattac ggatagcatg atgagcatgc cggccacggc ccgggccgcg aagttcattg
      661 ccatggcctg cagtcatatt gttagacttc attaaggttg tctcccggcc cctcacttct
      721 tcgcctgttg ccgggcgatc cgctcctcgc ccttcttcga gatctcgtgt tcctcccgcg
      781 gggtcagggt ctcgggggct cctggcgggc ggctcttctc acggccctcg ttgccgagag
      841 tgggggggac cgggctgtag acgatgtcgg tccccttctc ctcgaacgga atctcttcat
      901 caggccggtt gtcccgccgc ttcttcggat cgtctgccat ctgcatcact ccccggacga
      961 ccccgaccgc acaatcggga ctgcccgggg cggggttcgg ggtcgcaagt gtaaaatgtc
     1021 tgccggtgga ggtagagcct gccccccgga tcaccgacgc aggagaagac ccgcggccgc
     1081 aacggcgagg agcccgatga gcgcactcgc tcctccggcc cgggtcgggt ttgtcacggg
     1141 cgttgcgttc cccgtgggct cttccatcac cggcgcatcg gctgtccgga agatgaaggc
     1201 ccggtccccc gcacccgcgg cgaggtagtc gccgccctcc gagagggcaa ccgaacttac
     1261 gggtgcaccg gcgtcaaatg accagcgcgc atctccgttg ccgctccaga ggcggacggt
     1321 cccattggcg gttccggccg cgacggcctc gcctcgtcgg gataggtcta tgccgaggat
     1381 gtcgcttccg gtccggttct tccagagaag ggcgtcgttc ggccggtagc actccacgtc
     1441 gccaccttcc ttaccgacga ccacgaaccg tccgtccccg gagagagcga gcgcgaggat
     1501 tggcgctccg gtgggaagtg cccaggggag gccccctgcc cggccgtaaa cctggagggc
     1561 gcggtcccgg ctcccggcgg cgacgaccga tccgtcacga gagacatcta ccgcagtgac
     1621 ggcgtcgcgg gcgagcccct cccagatcag gtttccgtcc ctgttcatga agaggatggc
     1681 gccgttgctg ctcccggtga cgatgtagtt cccatcgttc gtgaaagcga cagacgtaat
     1741 gtacggtttc gtgtcgccgg agcgggcgcc cggctgcacg gtgcgattcc agtcctcctt
     1801 cccgaggttg ttgaagagga cgatgcgatc gcccgccact gcgagcgacc ggccgtcacc
     1861 cgcgaaggca aggtcgcgga cgggtcccgt gacgctcttc gtccagtaac ggccgccgtt
     1921 cgcgtcgagg aggaggacgt cgcccctgtc ggtcccggcg gcgacgtagc ctccggcgtc
     1981 tgcgacggca accgtggtta ccgtgcttcc ggcactcacg ttccaggggg cggcaccgtc
     2041 ccgctgcaga tagacgatgc tcccgccctc cgtccctgcg accaggtatc ggccgtccgc
     2101 ggtgatcgca acgtcgttga cgatgccccc cgtcgatatg ccgtggtagg tggcttccgc
     2161 cccggtagcc gccggaacca gggcgacgat cagcgcgatg atcagtgctg ttcttctcat
     2221 ggttaacccc tccgggggag agtatccagg aatatgtcta ctttcttggg cgggttctta
     2281 aactattcca gcaccaggct ctaccgcgca aatttgaagg ctccagactg gcaatcattc
     2341 cgtcaggtga atcacatgga gaacaggtca aaagaagcgg tctcccgctt catgcggggc
     2401 tacaactgtg cgcaggcggt cagttcggtc tttgcccgcg atctcggggt gcctgaagag
     2461 gtcatcctcc gggcggccac cgggttcggc ggcggggtgg ggcataccgg gggagcctgt
     2521 ggggccgtct cgggagcggt cctcgtcatc gggctcctct tcgggagcac cagtcctgag
     2581 gaaaaagagg cgaaggatcg gacgtatgga ctcacccggg aattcatcac ccggttcacc
     2641 cgcaaacgtg gcactgtctc ctgcaccaaa ctcctcggct gcgacctctc gaccgacgaa
     2701 gggcttgccc gggcccgtaa gcagaacctc acccggaccc tctgcccccg ttatgtcgaa
     2761 gatgcgaccg agatcctcga agaggttctc gcgtccgtca cttccgggca atccaccatg
     2821 cggtgagaaa ctccggcagg gacggcagcc tgagcacccg ccgcaggtcg gcggtgccga
     2881 agttctttgc cgccagcgcc cgcttctcga cgatgtgcca ggagaggaac gccaggggga
     2941 acgccaccag gaacgagagc ccacagagtg ccgggaggag gagcgtgttc gccgtgacct
     3001 ggatcagcac ctgctgaatt gggtagtgat agatgtagat gccgtaggag aagtccccgg
     3061 gcttcccgaa ttcgttcagg aacgggaccg ggagatgtgc agcgtagatg acgaggtagg
     3121 ggatggcgag caccccggcg acggtcaggt agggcgtcag ggccgagagc ccgagcagca
     3181 ggagcagtgc cccggcgatg accggccggt aagcgatgcg ttcctggtga aggtagaggt
     3241 aggccccgac gaggaagtag agggtgaacc gaatcttcgc catcctgggg tcgtcgaacc
     3301 aggcgatcca caggaggatg tttattccca tgacgccgag gagggcagat ttccgccaca
     3361 gcaacccggc gatcccgagg agcgcgagga ctccgtacat cacgacctcg acgggtatcg
     3421 tccagagcga gccgttgacg taggtccacg ggttcgcctg gaagaggcct atcgccgacc
     3481 cgtcctcaaa gaacggggcg gtggcgagcc ctgccggaga gaagaggacg ccgaagtatc
     3541 cggccggagg cagggacgtc atcagcggcc cgatgacgaa gagcgtgatg aggatcgcga
     3601 ggacgagggc cggaacgacc cggaggaacc gtttccaggc gaaccggagc ggtgatgcgg
     3661 tcgactccca gctcgccgcg atcaggtagc cgctggtgac caggaggatg gcaagccccg
     3721 cctgccccat gagcgcgacc gggtcgtaca tccctatgcc gacgtagccg agcctgagcg
     3781 cgtaggcgtg cgtgacgacg atcaccgccg ccgcggcgaa ccgcaggaag tcgaagttgt
     3841 tcgagaaccg gcccccggag cccttgcctg tatggctgga aatgcccacg ccttccgggg
     3901 tcgctcccgg gtgctctgtt ttcatagcga tcgggatcct ttcctcactt attcattgac
     3961 aattcttaaa taattagtca aaatactctt ccttatggac ccgctcgttg tcgccacgct
     4021 cgccgtcgtc gccctgctcg agatcgtcgt gccgcttgca ctgggctact ggatcgtcaa
     4081 aaggttcggt atctcctgga aggtcttcgc gctcggcgcc ctcttcttca tcatcgtaca
     4141 ggtcgtccac acaccgctcg tcctcgtaac acagaccccg ttctacctcg ccgtcctgcc
     4201 gttcgggaca accgcggccc tcgcggtcct cgccgtctac ctcggcctca tggcctcctc
     4261 ttcggggtcg gttggggcgg cgtcgagagc atgatcgttg cactcctcgt gctcacgggc
     4321 atgctctctt atatagccct caccggtgat gggtcgatgc ccttcatccc cgacgatccc
     4381 gccgttcagg cgcaggtcga cgctctccag gccctcacgc cgctcgatat cctccccggg
     4441 ctcgccgagc ggatgatgac gatcaccctg cacatcgcat ggtcgctgat ggtgctcgca
     4501 tctgttgttt acggcagaaa ggcactcctc gccctcgccg tcctctggca cgccgccgtc
     4561 tacctcgccc cgacgcaggg ggtcctggtg accgaggcgg tgatattcgt ctttgcggtc
     4621 attgggcttg cgtatatcct gtgggagtgg cggcggatgg gggtaagggc tgcttcttag
     4681 cccttccact cgtgcaggta gccccggcgt tcgagcggct tcccgtccgc gaggaccgtc
     4741 cgctccacta cggcctcgcc gatgacatgc gtctcgaccc cctggaccgg gaggatgccc
     4801 ggaggggcac agaagaggag ttcgaagtcc cctccaccat agagcgcgag tgcccgcccc
     4861 tcgtcctccg ggacgccggt cgggagcggg agccgggcgg tatcgatcga gaaaccgcag
     4921 tcgttcaccg cgaggaggtc atggagggag agggcaaggc cgtcggagat gtccatcatc
     4981 gccgagaccc cggcccgtcc gagcgccagc ccctccttca cgcggggctg gggctccagg
     5041 agttccttca tatgccggcc atagccgttt aaggccgcct gtgcctcccc gagcggcccg
     5101 gtgaccgcga tgacgtcgcc cggccgcgcc ccccgccgcc ggacgaggta ttccggggcg
     5161 acggtcccga ggccggtgct cacgatcgtc agttcccggt gagcgtcaag gtcccctccc
     5221 acgagttcgg cgccgaacgc tatggagcag tcccgggcgc cctccatgat cccgcggagg
     5281 cgttgtggcc gatcgaggcc gaccgcgagg agcacctgcc ccggcgccgc gcccatgctc
     5341 gcgatgtccg agagcgtgac cgccaccgcc atccagccca cctgccagtc ggtcatcccg
     5401 gcaggaaaat ctgtggtctc gtggagcata tcggtgctcg cgaccatcgc gagattcccg
     5461 caggggatga cggcgcagtc gtcctcgagc cgttccgggg cgatgattgt cccgatcagc
     5521 cggtgcaggc tccgctcatc caccgcgacg cacctccttc tcccgctcgc tccggtactc
     5581 cttgaagagg tactcgaccc gctcggcctc ctgctcgcgc cggtactcct tctgctcctc
     5641 ctcccattcc ccgatggcct cctcgatggc ccgggtcctt gcggccccgg tcctgccccg
     5701 gatatccggc ttcacggtgt ccgcggggag gagggggaga ccggcctccc gggcgacccg
     5761 gaccaggtgc gggtcgggag gctcctctgg cctcccgccg accaccagcg cccgcacccc
     5821 cgttcccgca aggtctttca ccacgccccg tccccagccg tgggcccggg ggacgtagag
     5881 gatgtctcct ttaacgatgc cgacctcctc ctggaggccc cgcaccccct cccgggtgag
     5941 ggaatcgagc accttgagcg gggtgtaatc ctcggagacc tcgatcttcg cgacggtctc
     6001 tatccgttcc aggcgtttct tcagccgccg ggaccgccgc cgttcgcccc ggagctgctc
     6061 gcgcaggctc tcgatggtcg cgtccttcgt cgccagttcc gcgtcacgcc ggactttccg
     6121 ctcggtcgcg gaacgtgccc gccgcaggct ctgccgcagc cgctccatct cccggtcgtg
     6181 ctcccggagt tcctcctgca gttcctggac gtaactgcgg agccgtttga ccatcccgtc
     6241 gagcgccatc acccgctcgt catcgattgg ccgttccggg acaggcaccg tgggttcgac
     6301 tggttttggt gccgccggga ccccctgcag gtccgcgagg accgtatcga gcggctggcc
     6361 ccggagcacc cgcgcccgca cctcgtcgag gtcgaacccg ggctgcactc tcttcgtgat
     6421 attcctaaac ttgttctgga ggctccggta cgcgtccagc gccgccgaga gagcatcgcg
     6481 ctcgtggtcg ttcgtgtagg agaacgcggc ggtcaggtcg tacttcgctt ccaccgagag
     6541 cgactgcttt ggagtgtagg gaatggcgtt gaacgcccgg cggatcttct cgaccgagta
     6601 gggcatctgg tggacgtcgg aggcgatgat gagtggtttt cccgcgtggt agagttcctc
     6661 gacgatgtcg gacatcgtca tctgccggga actcgagagg aggaccaggt tgccgtcgag
     6721 atccacggcg gcgatgccgg tggtcgttcc cgggtcaagc ccgacgatca ggtagcgggg
     6781 gcgactggag agcggttcga accggatacg gtcgagttgt ctgcccgcca cccggacctg
     6841 gacgtcggcg ccccgggagg agtggaccgg gaccatgtcg cggggagcga cgacccggaa
     6901 ggtgacccgt gagaaaccac cgaaggcctt cgtctccttc ttctcgtagt cgagaccggc
     6961 gccccggagc cgtgcctcga tctcccgggc cttctgcagc accccaccgt ggatcttacg
     7021 gacgtagcgg ttctggctcc acccgccccg gcccggcgat cggtgcctgc tcaccacgat
     7081 gtcggtggtg ttctcaaacg cgatcacctc gacgcctgcc ccgagggagg ccacccgggc
     7141 ggtggtccgg gcctcggcgt aggggtcgaa cttgttgaaa ctgatgttgt aacgggcggc
     7201 gactttcccg agactctctt tcttctcgcc gccggtcacc tggacgagtt tcgttgcggg
     7261 cgggagcgcc tgcaggaatg cgtagagttc gcgctggtct gcagcgatct cctggaggct
     7321 gtccaccgcg aggatgtcgg gctgctcggc ggcgaggagg cgctggagcc ggaaccccgt
     7381 gacctcctcg acacccatta tctcgccgtc ttcaacccgg gagagggcgt agacgggccg
     7441 tcgggtacgg gaacgaaccg agcctttgat gatgtcgatc ccgaataccc tcactccacc
     7501 acccttgcca gtgcttcgtc gaaggggtat acgagggcgt atttccgctt gaagtactgg
     7561 aggatgttct cgatatcgcg cgcgagggtc tcgccggcgt tcgggtgagc ggtctccacc
     7621 cattggggcc agtcgatcag ccagcactgc ttctcgtcga ccatgatgtt gaactcgctt
     7681 aagtcaccgt ggacgatgcc gaggcggtag gcctcccgga cgttatcgag gatctcatcg
     7741 aggataagct ccggctccgc gacggtcgcc cgcgagaggt tgatcccggg gacgtacgac
     7801 atcgccacga cgtgtcggtt ctggtctatg ggaagcggta ccgagacggc aggatggagg
     7861 gcttttaggg cgtcgtactc catcttcgcc gagttgctcg aggcgaagag ccaggggcag
     7921 tggccggcct ccggcatgta gccccgcttg acgcgggctg actggaacga ccgctgcccg
     7981 acccggtgga acttcagcac cacgaccccg agtcccatcg cctcgtagac ctccgactct
     8041 ttcccgacac cgatcagggt cccgagcgcc tggatgctgc cccggcgggt gagggtgtgg
     8101 agggccaggc tgtcgtagcc gttgaagacg agggcgtaac cctcgtaggg gactttctcg
     8161 tacctgacca tattcatggt catcagcctg ccaatccggt aggagagttc ggactccgag
     8221 agtttggtgg ccgacttgag cacatcgagc ggcacccact ggtaccgccg catgaggcgt
     8281 tcgagggcga gcaggatccg gagttcgtag gcgtgcaggg atcgcacgta ttctgcagga
     8341 acaggcatct tctacaccat ttatggtgct cggtgataaa aggatgatcg agaacgcatg
     8401 cagtgcagca agtgccgccg cgaagcggtc atctaccagc gatactcagg gctgcacctc
     8461 tgcgagcagc acttcaaccg agatttcgag gcgaaggcga agcgggcgat ccgggagcac
     8521 cgctggatcg agtcggggga tacggtggcg gtggcgctct cggggggcaa ggactcgagc
     8581 gctgtcctct tcttcctcca ccggctcctt ggcgagcgcc gggatgtccg gctgatggtg
     8641 ataaccgtgg acgaggggat cgggggctac cgcgacccgg gccaggcccg ggcgatcgcc
     8701 gacagagtag gcgtcccctg ggtgacggcc acatttgcgg aggcatatgg gatcacgctc
     8761 gatgagatcg tggcccggaa ggggacgggg ctctcctgct cctactgcgg ggtgctccgg
     8821 cgggcgctga tgaaccggat tgcccgcgag gagggcgtca cgaagttcgc ctacgggttc
     8881 aacctggacg acgaggccca gtcggtgctg atgaacgccc tccgggggga tgcggagcgg
     8941 ctcacccgcc ccatgcggga ggcggagggg atggttcccc ggatcaagcc gttcatgtat
     9001 ataccggagc gcgaggtggc gctctacgcc ttcctccacg tcgagggctt cgaccttgcg
     9061 ggctgcccct atgcggggga cgctcttcgg ggcgacgtcc gcggcatcct cgacgactac
     9121 acctaccgcc acccggcgac gaaatactcg ctcgtgaacc tcggcgagac tctccgggag
     9181 ccggcgaaga atcacaccga agggctcaga gtctgcgaac ggtgcgggga gccgtgcggg
     9241 aagatctgcc ggacgtgtca ggtgcttgat gaggttcgga agggtggctg atagagactg
     9301 ggtgcatcta tcggtggcga ggaacgggac tgtccaaatg atgatattgc ccggtacagt
     9361 ggaccgtggg gacatcgcca cgcactgccc ccgcccctcg gggcgggggt acagtgtccc
     9421 cttatgttgc agagacaggg gaggggggcg agcccccctc cccgtcagcc cctcccccgc
     9481 gtggcgatac ccactggaaa tccgtgcacg ggatgtgcgg tgttctaaag aacaacaggt
     9541 ggtagagttg aacggcgctt gaccctctct tcaacagcgg tcttgccctc taccgcattg
     9601 ccagacatcc gccggtcacg tcggcgaaga tctccatgaa ctgttcgggg tggttcaaga
     9661 acgcgaccga ccggggacgc ccgatcagat catagagccc gatctctttc tcgatcccga
     9721 ggtccgggac ctccaccggg ttgcagtagc gcaccggcgg ggcgtcgcga tagcgggggt
     9781 tcttcaccag cgccccgccc tccatctcgt agtaggcggc gccctgcagg tccgcatagg
     9841 ggccgtagtt gctggagaac tccgacgaga ccaggtttgc catcacgaga tcctctcgtg
     9901 cggggttgat ggtcacgtgg ccgtaccccg gcgggatcag gatcttgtcg ccgacggtgc
     9961 cctcgatcat caccacgtcg tcggcgttct gggtctggag gaggtagtgc cccgtcccct
    10021 cgagcacctc gtagatctcc gggtagagtt ctcctgccgg gttcgcggtg tggtagtgcc
    10081 cctttgtctt gatgtactcc ccgcagaggg tccgggaagg aatgatggtg atgtcgtagc
    10141 ggacgtggtg ctgccggagc cagttccggt cgctctcgct ccgggccagg tcgcggtaca
    10201 taaaatagag gggctgatcg gaggtgcagc cgggatctgc gagaacctcc cgcatctcct
    10261 cgattgtcct cacctgcggc tccggtttag ggccgtccca gtacccgttc ataataatca
    10321 ggcaggagac c
//
LOCUS       DAJB01000033           13184 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_38253, whole genome shotgun sequence.
ACCESSION   DAJB01000033 DAJB01000000
VERSION     DAJB01000033.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13184)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13184)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13184
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_38253"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2661 a         3950 c         3987 g         2586 t
ORIGIN      
        1 ccccgcgagg cgatatcccc acggtccact gcaggggcat gcccatggtc ccacacagaa
       61 ggcaggccgt gagggatgcg aagggtggga aagggaattc atagacttaa gtgacaacga
      121 ccccaacgcc gtccgggacc ttaccccgaa atcataccgg cggtgcgagg agacgatcga
      181 gaggagagaa ctcgatatgt ttgacaacaa gatagcgtcg ggctctataa cgacgaatgc
      241 tggtgaacgc ggcgccccat catttgccgg atcccgtacg ggacatcgca gggatgatac
      301 gggccggcag aacggaacct gcggtcacgg attggagcta cagaactgaa actcaaaatc
      361 gaaaaacgcc ccggccggga tttgaacccg ggtcaaaagc tccgcaggct tctaggatgt
      421 ccactaccct accgggacac gagactgagt gtctttcccc taataactac gttgtcctgg
      481 ttaaaaagga ttgctcccgg gatgatcgga acgccatgga gagggttgca gggcccgaca
      541 tctgcgggga taaaacccga ttcctggcgt atccgggagg gagcggtagc actggagggg
      601 cctacaggcc tcctgcgcgc accagtatgt gtacctttac atcgtttccg tcgaactgga
      661 tcaggtattc tcctgaccgg cctacgaaga cccttttatg tgtatctgcg ctgaagagtt
      721 tcccgaaacc gtcctgggcc acaatcttgc cggacccgga gtccctgaca gtcactgtaa
      781 accacgattc ctcgctcggg taggtctgcg tgtagtcttg atacttcctg tccttgtagt
      841 cgctctttgc atgcatcacc cgcgtgatca tcttcggctc cacctcgaag tcgatgatga
      901 gcgggggcgc ggtcaggtcg aaggagaagg cttccgttgc atgagtggca ttgaactgtg
      961 ccgtccggtt agagagcgtc acgtaggggt ccggcgtcgg cgcggtctcc ggcggcggac
     1021 tgctgagtgt ggggctggcc atagtgctcg ttgcagtggg atacggggtc gccggcgtca
     1081 gataccccgg atcggcatct gacccgcccg gtgtctcctc cggttccgac tccgaaagga
     1141 ggccggacca gccggattct ccgccgggcg gcaactccgc gcacccggcg gatgcgagaa
     1201 tcaggaccat cactgcaact agaaagaacg atttatacac ccgcgtcacc gtagtgggat
     1261 tatgtgaaga ggtatttatg cttttatgct tgaatacccg gagaaaaatt ttatacaaga
     1321 tatatcagtc agcagaaata taattggaaa ccgacctgtt tttgcggtat aattccggaa
     1381 gacgcggctg gatcagaaaa atagacctct tgtgagagac tcccgggaga ggtcgagcat
     1441 atgttcgcag agtcctctgc agtgcaggaa cctttttatc agacgtttgt acctctatcg
     1501 cctgaccaat gacagtaccg gagagacaga agacacaacc gatagacacc gaccccccta
     1561 ttctggatga cagcagcgac cctgatgaga gcgcaaaaac gtggcgtgcc actccggaaa
     1621 acgacctgaa ccttaccggg aacctcctga tcctcatagg tctgaccggt atcggcgcca
     1681 tactgcggct ctacggcctt ggaactaacc cctcgtgttc gcccggcagt cgctcattgg
     1741 catctgggag agcacggtcg gcggagagtt caacccgccg ctcttctact ggctggagca
     1801 cggggtgctc ctcttcgggg atagcgagtt cgtgctccgg ttcctccccg cactcctcgg
     1861 cgtcctgacg atcccggtcg tatacctcat cggaaaagag ttccggaaca aaaatatcgg
     1921 gctgatcgcc gctgccctcc tcgcgttctc gcccttccac atattctact cccaggaagc
     1981 ccgggcgtac gccccgatgc tcttcttctt ctccctggca ctgctctgct acgtgaaagc
     2041 aagccgatcg gacgagaccc gcccctgggt cctcttcggg ctcttctccg cagtcgcctt
     2101 ctggatgcac ttctacgcca tcgtgccggt cgccgtcctc atcctccacg ccctcctcac
     2161 ccgcgccggc gagatccgga gcgacgtcag aagcgcaagg aacctggtcc tctcggtcgt
     2221 cacgttcgtc gtcgcaagcc tccctctcct catcgtaacg atcaacctct tccttgtccg
     2281 gacggcgtcc gctccgacct tcggcatcca ggggcccggc gtcgtctacc agacccttct
     2341 ccagatctcg gggtccagcg actggatcct cattcccttc ctcatcctct ttctgatagg
     2401 cgtcacgtat acctggcgcg aggaccggga tggagcgctc ctcctcatcc tcatgatggc
     2461 cctcccgctg gcggcaagcc ttgttctctc ctcgaggatg ccgatgctcc cgcgctacct
     2521 catctacctt ctcccggtct acttcatcgg cgtcgcatcg gcgtatcccg ggctctacgc
     2581 cctcgtgaga gacgggaggg cggtctacct cgctgtcgcg gcagcggtgc tcatcagcac
     2641 tccctttctt gcgacctact acacgacccc ccagaaggac gactggcggg gtttctccac
     2701 ggaacttgcc gggatgaccg gggaaggcga tctcgtcgtc gtgctgccgc cctacatggc
     2761 gcagccgctc gattactatt acagcaacgc gaccgaccgg accctcgaaa tcggggcaac
     2821 gacccggaag gacctcgagg cgatccgcga ccaatacccc gaccggcgtg cgttctacgt
     2881 ggtaaccctc gatatctccg ccgcggaccc caccggagat gcggtgggct ggctggaaga
     2941 gaacacacgg gttgtcgggg agcacacggg gatccacctc tttgtgtcgg ctcagggaga
     3001 gttgaatgac tgatacctgc gacctgacgg tgatcgtccc gacgttcaac gaggaggaga
     3061 atatcgccgc aatcgtcgag gcggtcaacg gagtcttcct gcagagcggc atccgcggcg
     3121 aagtcctggt ggtggacgac tcctcgaaag atcgcacgat cgagatcgtg caggagatcg
     3181 ccggcaaaaa cagcaacgtg cggctgatcg tgcggaagga ggaccacggc ctctcccagt
     3241 cggtcgtcga ggggttcggg gcggcgcgtt ccggtatcct gcaggtcatc gacgccgact
     3301 tttcacaccc gccggaactc atcccccgct tctacgaggc gatacggggg ggagcggata
     3361 tcgccatcgg gagcagatac atgaagggcg gggatatcga ggactggccg ctcaagcgga
     3421 gggtcatatc gctcggggcc acggcgttcg ggcgcatact cttccccgag atcaccgatc
     3481 ccgtgagcgg attctttgct ctgaggcgcg aggtcgtctc cggcgctccg ctcacaccgc
     3541 ggggctacaa gatcctgatg gaggtcctcg gcaagggccg atggcactcg ttcgtcgaga
     3601 tcccgttcgt cttcaaggac cgcgaggagg gggagagcaa actacggccg gacaccattc
     3661 tcgattacct ccggcagtgc gccgatatca tccgcttctc ggccacgcgc cgggccgggc
     3721 cggtctatgg cgagtgggag aggatcgtcc ggttcgggct cgtcgggctc tccgggatcc
     3781 tggtcaacat ggggctcctt tacgccctga ccgaggttgc cggcctctac tacctcgtct
     3841 ctgcagcaat cgcgatcgag ctctcgatca tgaacaactt cgtctggaac gacgtctgga
     3901 cgtttcggtc tgcaaaggac ctcagaatcg ggcggaatct cctgcgcttc tggtcgttcc
     3961 aggcggtctc gatgggcggg ctcgccatta atgtggccat cctctacctc ctcgcggatg
     4021 ctgccggggt ctattatctc atcgccaacc tcgccgggat actcaccgcg tttgcctgga
     4081 attatgcggt gaacaggcac tatacgtgga ggggggcata acagggagct ctctctggaa
     4141 gaccggaggg gctccctcct ttccggataa tggaaaaatg gtgtccggtt gatatcgaac
     4201 ggggccgtcc gctcagagag aatacgcccg gatgacgccc cggtagacgt ccactgcctt
     4261 attgagctgc tccactgaaa cccgctcgtc caccgcgtgc agcgtcggga tctcccccgg
     4321 cccgtactcc acgacatcga agcccctgtc gcggaggtac ttcgcgtcgc ttgcagccca
     4381 ctggaggaac ggcatcaccg tcccgtagac ccgttccacc tcgcgagaga ccgtccggac
     4441 aacccgggca tccggggacg tcagcgtcgg ctcggcaaca tccgtctcgt gtatcacggc
     4501 atccggggca tgttcggcga tgcttctccg gaggtcgtcg agggagcagc cccacggcac
     4561 ccggatgtca agctccatcc ggcactgctc cgccacgata ttggccttct caccgccctc
     4621 gatgcgcccc ggattgaaca tcagccttgt caggatgttg tcccctccct cgatcccgaa
     4681 gatgtcgcgg aagatccggg cggactgtgc gacgagcggc tgcaggtctt cggtgacggg
     4741 gaacgggcgc gcgtggacct cctgcaggta gccgaccagg tcgaacgccg ccatgatggc
     4801 gctcttcccg accaggggat agagggaact atggcccggc ctgccgtgga acgagaggtc
     4861 gacccggtag aggcctttct ggccgactgc cgggctcatc gccggcgtcg gttcggctat
     4921 caggcagtcg tggggcagga gcaggtcgtc tgcaagcagc gaccggatgc cgtgctcccc
     4981 gccggtctct tcatcgcaga cgaaggcgaa ctgcgccttc ggctccaccc cactctcgag
     5041 gaggtcgcgg taggcgatga ggagggcggc gcatcctcct ttcatgtcgg tggcgcctct
     5101 cccccagacg tagccgtcgg caatctcccc gctgcagggg tcgtgcgtcc agtcgtcggg
     5161 gatggcgggg accacgtcga cgtggccgca gaggaggagc cgggggtccg cctcagtcgt
     5221 gacgaggttc tcccgcccgc ccggatacga gacgatgcgg ttcttcaccc cgagcgagtc
     5281 gaggaactct ccgatgaatg cgaccaggtc ggccgtactc cccggcgggt tctcactcct
     5341 gatccggata agcgatgaag tgagtcgggc gacatccatg cacggtctcc ttatgcagat
     5401 ctgctccgcg cgtactcgtc gaagagccgg gcgagcgctg aggtcccata gagctgtttg
     5461 acgaacttcg ggtcaaagaa ctcgtcgacg aacatcgaga ggtaatcggc cgggattgca
     5521 ctcttgtcgt cggggttgag tacgatgcgg aaactcctgt actgggcggg gtcgtcgctg
     5581 tcgtagacgc cgctccagag catcgggagt tcttcgaaca tctccgggtg ctcggtcttg
     5641 agccagttga tgaacccggg gaagtatgcc cgctccccga ccttcgcctc atccacgcgc
     5701 cagcgcatat actccatgct catgatattc cggggcgaca tgtagttgta ggccaggatg
     5761 ccgagggtga agtcgatgtg ggcgagggca tcggtgaagt aaaagttcag gaccgggtgg
     5821 aactcggtag gactctccag gatcagcgat ttgccgtaca gtttctgcat gacacggaca
     5881 tactcgttca tttccagcat actctctcct attggggaga gtggtacaaa aagtattcct
     5941 tgtgtgcagg cagggatggg gccggaaacg ccgggcgggg ctccggcgat gcggggtttg
     6001 ggccggtatg ggtggcggtg aaaccgggcc gggtgggcgc cggtgttctc accagacgga
     6061 gattgggctt tgattggccc taggggccgg tcatcgccac tcgaaaacgc gaagcgtttt
     6121 ctcaaactcc gcctgccccc cgggcacgcg tcgctgctcg cacctccggt gctcgaactc
     6181 cgttcggccc attgggccga acctcgttta cacaaagaac ggcttgcgtc tgctgacggc
     6241 ttccctgaag gcgtcttcca gctccttgcc gtgcttgccc gtggtatcga tcaggttatc
     6301 gctccggagg aggcagggct tgagtttgcc gtccgaggtc acgcgcaggc ggttgcagaa
     6361 tgcgcagaac tccgtattgt ggagagggcg gaccacctcg atctcggcgc cgtcgaggca
     6421 gtacttcttc cggtggtgca tcctgcgggt gacgacccgg gtcgcccgat cgtttaagtc
     6481 ccgctcgacg ctatccacgt ccccgtggaa cttgcactcg ttgaactcca tcaactcgat
     6541 gatctgcagg atgaggtcct ctttgtcccg gacgaacgcc atgaaatcgt ccagttcatc
     6601 ctcgttgatg ccgtcgagga gcaccatgtt gagcttgacc ggggtcagtc cgacctcgat
     6661 cgctgcatcg atcccggcga gaacgtctgc gagacagtct ttccccgtga tggcccggta
     6721 gcggtccgga cggagcgtat cgaggctgac gttcacccgg gcaagcccgg cctctttgag
     6781 ttctgcagcc tttgcggcaa gcagcgtccc gttcgtcgtc agggaggact cgacgcccgg
     6841 cggcaccgag cggacgatat cgacaaggtc acggcggagc aggggctcgc cgccggtgaa
     6901 cttgatactt ttaacgccga actgcacgcc cacccgtatc agttcggcga tatcctcggc
     6961 actcatctgc tcctttgggc ttacctcacc ctctgcgtgg cagtagatgc accgcaggtt
     7021 gcaccgggag gtcaggctga tccggaggtt ggtcacggtc cggttatagg ggtccttcag
     7081 caccatcgca tcttcccttg aaattctttg ctataatgta ggtctcagca ctccctttcc
     7141 gtgacgcctt cgtcctgtac ccccgaacgg aatagaaatg cttccttgcc tcggtcatca
     7201 attccccgaa gagctcaccc tggaacgact ttattatcat gttgccgccg ggctttaaaa
     7261 gcgtgcaggc aaacgccaga gcatcctcgc caagcccgat tgcgcgagcc tggtcgtagg
     7321 tcttctggcc ggagagtttc ggtgaggcat cggagaccac gatgttgacg atcccaccgg
     7381 cctcctcgcg gatgcgctcc tgaacaagag gatcggtaaa gtccccgacg atggtggtga
     7441 cgcctccgat gggcgcgatc gggttgaggt ccacgccgat gaccttccct ccggtcagat
     7501 cgcggagcac ctgcagccag ctgcccggcg ccgccccgag gtcgacgacg ttatcactgg
     7561 gtctgatgat atcgttgcgc tgctggatct ccagcagttt gtaggcggcc cgtgcccggt
     7621 atcccgcctt catcgccttt ttatagacgc tgtcttttga ccattgagaa cccattgcga
     7681 gattaccctc cacttgaggc tgacagatgt acttgaccca tcaagcatta tcaaggagat
     7741 tatggatagt gtttgatgcc gttaatcatc aatgatacgg ttgcggagta tgggcgaccg
     7801 gagtctgctg tgccccctcc cacgataggc gccatgggct ttctcccggt tttgatcgta
     7861 aagcactata atgaaaagtg tgattatata ctttagacga gagtttaata aggggtcaca
     7921 atgttaaagg caacgattga tgcagaaata tttcgggaat ccatcgacgc gatcgccgca
     7981 ctggtgacgg aatgccgcct acacacggcg gaggaccaga tcaggacgcg ttctgtcgat
     8041 acagccaacg tggccatggt ctccctggaa ctccagagca cggcgttcaa ttctttcctg
     8101 gcgacaaccg gtgaactggg cctggatatc gcaaagatga agaacatcat cgggatgatg
     8161 gggaaaggcg acgcgctgac gctcagtctg ctcgatgaag agcggaaact ggaactgagt
     8221 tttggaggct accgctactc ggtcacgctc ctcgacgtca acaccatccg gaaagatccg
     8281 aatccaccgg gaatagaact ccccggcaag gcgatcatat cgggagacgc gctgaacaat
     8341 gccatcaaag cagctgccgt catctccgac aagatcgcgc tcgggatcga ccccgatacc
     8401 atgactttct acatggaggc ggagggagat accgaccaca tcaaactcgc gctcggcgaa
     8461 gaccagctcg tcgcgctcaa cccggtgcgg gcccgttccc tcttctcgat cgattactta
     8521 aaggatatgg gccgggttat ggcacgtgcg ggtgaggtgg aggtctacct cggcatcgac
     8581 catccggtac gatttgcctt tgacattgcg aacggcaacg gccgcgtcga gtaccttctt
     8641 gctcctcgga ttgaggccga ttgatggata ttgtactcga ccgcaaagaa ctcattaaat
     8701 accctttttt gaaagaatcg cagcagctgg cccgccggca cgttgagtcg ctcgaagagt
     8761 ttctcaccag cagcccgggc gctctggcgc tcgagcgggc aaaggagcgg gtcatcgctg
     8821 cgcttacgtc gaagagggag tttggggacg agaatacgca gaatctcaag cccgagtttg
     8881 agatcgcgag ttatgcgctt gcccggatac tggtctcctg catcggggac cgatcgctta
     8941 tcgaccgtct tgcgcgctac gaagcagaac gcgcatcctt cttcctcgcg accgaggacc
     9001 cggagatacg gcggttcgtt gcctggagca tcggcatcag tgccacgacc gctaccctgc
     9061 cggtcaccga ttacgtggaa ctggtgcccc atctgcgcga tcggcgctgg cggctcgtca
     9121 acagggacgt gcagcaggga ggcgtgaacc ttgcgccggg cgagatcgat gaactcatcc
     9181 gggaacggat ccgggtcatc ctggtcgatc aactgccgct ccgggtgccg tccgggatct
     9241 gcgagcgtct tgatgcggtt acaaaagaga tcgttgcgtt gcagcaggag cagatcctcg
     9301 agcagttcgg cgagatcaag gaggaggcgt tcccaccctg catcaacgcg ctcatccagg
     9361 cgatcacctc aggagcgaac ctgacgcata tgggccggtt cgccatcacg tcgttcctcc
     9421 ataccgtcgg gatgaacacc accgggattg cggagatctt cgcgcgggcc cccgacttcg
     9481 acctcgatat gacgatgtac caggtggagc acatctccgg ccggggcggc accgagtata
     9541 ccccgccgtc gtgcgccacc atgcgcacct tcgggctctg tgccaaccgg gataaggact
     9601 gcgagcgggt caaccacccc ttgagttatt accgcctcag gaaaaatata aagaagaagc
     9661 ggagttaagc gactttttta tcgatcagat agcctgcaat actgagcgcg gcgataaagg
     9721 cgaccagcgc gaagacgtat gcaagccctt ctatccccac catcagtgcg ggcagcgtca
     9781 ccagcagcac gacaaacagc gcaaggcacc cgaatgcggc cagcacctca agaacccgta
     9841 gattcatgta cagaggtagg gtaagggcag gtatatctct atccccgggt gggagaccca
     9901 catatttatc ccgggcgcgg gagaaacccc tctcgtatgg agagagacga ggcactggcc
     9961 ctgctccggc gctatgtggc gtcgccgtcc cacatcgtgc acagccacgc cacggcggcc
    10021 atcatgagga aggtcgccga gcacctcggc gaggatagag agacgtggga ggtgatcggt
    10081 cttttgcacg acatcgacta cgacatcgtc ggaggggata tggagcggca cggcatcgag
    10141 ggctaccggc tccttctcga aaacggtgtc ccggaggaga tcgcggatat cgtcaggcgc
    10201 cacaaccacc tgctcacatc cggctacgag cgcccggtcg aggttgcgct ccaggcagcg
    10261 gacagtgtat ccggtctgat cctcgcctgc gccctggtga agggcggggc gatcgatgag
    10321 gtcacccccc ggacggtgaa gaagaagttc aaggagaagt cgttcgccgc gggctgcgat
    10381 cgcgagagga tccggctgat cgagtctctc atggatatgg agacgttcta ccgtctcgca
    10441 atagaagggc tcacggaagt tcgcggcgat atcggccttg cctgagcaga acacatatat
    10501 ggcacccgac gtgaggggta cagcatgaca tcggaagacc gcgggagggt tatcgaccgt
    10561 ctgggagagg ctgtaacctt gctcaccgag cgaatggacg tccggctcat cccacaggtc
    10621 gggatgaaca tcgtctacgc cctgcccgac gcgcggagca aggacgacgt ggcaggtgtc
    10681 ctcggccgga tcgtcaggct cggtgaggcg gtccaccccg tcggggagat cgcgttcggg
    10741 gcaagcgatc acgttgcccg gatcgtcatc acggccatgc acgttaaccc gcaggtcagg
    10801 agcgcggcaa acatccgctt ctcagacgca atcctccctg agctggataa cctcctcttc
    10861 gaggtctgct cctttgaccg ggcaaaagaa ccgccggggg tgcggacgat ggactggggg
    10921 gtcgcctcct gctgcaagga gggcgtgccc gacgtcatct acgaccgggg ggctatgggg
    10981 aaagaaccga tgatccgggt cctcgcggag gatccggtca tggtcgcaca aaatgttctt
    11041 aaactgtcga atcgcattat gtatgcacaa ttataaggga aggtccacat gggaataaag
    11101 ccatcatata tcaagaacct cggcgaggaa ctcattgtca agcatcgcga cagattcagc
    11161 ggagactttg atgaaaacaa acacgccgtc agtgaagttg ccgtgattga cagcaagtgc
    11221 gtccggaaca gagttgccgg gtacatctca agaaagataa atacagggaa gcggtaaacc
    11281 tcactatatg tttgagggaa tccttccggc aatcattacc ccttttcacc gggactccga
    11341 ggcgagtctc gatattgaag gattacggtc caacatcgaa tcactgctac aacgcggagt
    11401 caatggcatc gtgccctgtg ggtcgaccgg ggagtccgcg acgctcacgt tcgaggagca
    11461 cgagaaggtg atcggcgaga ccgtcggggt cgtcaacggg cgagtgccgg tgctcgcagg
    11521 gacaggatcg aacaacacgg cagaggcggt tcgcctgacc cggtcggcgc aggatgcggg
    11581 tgcggacggc gcgctcgtca tcagcccgta ctacaacaag ccgaaccgtt ccgggctcat
    11641 caagcatttc acgaagctcg ccgatctcga tctcccgatc gtcctctaca atgttcccgg
    11701 ccgtacgggg cagaacctcc agcccgacct ggtggtcgaa ctggcggagc acccgaacat
    11761 cgtcgggatc aaggaggcaa gcggcgacat cacccagatc tctcgtatca tcgaagggac
    11821 gcgggacgag gagttcatcg tgctctccgg ggacgatgca atgacccttc cggtccttgc
    11881 cctgggaggt gcgggcgtga tatcggtggc cgcgaacgtc gaccccggcc ggatggtgca
    11941 gatgtacgaa tctttccgtg caggcgacct tgcccgcgca caggacctgc actacaaact
    12001 cgctccgctt atgcgggcga tgttcatcga cacgaacccc atccccgtca agaaggcggt
    12061 ggaactcatc gggatggccg cgggaccggt ccggctgccg ctcgacgagc tcgacgaagc
    12121 gaagacagaa caactacgaa aggtgctggt aaactatggt taaggtagtc gtatccgggg
    12181 ccctgggacg tatgggcacc atgatcggcc gcatcgtcaa cgaggctccc gatctggaac
    12241 tcgtcggcgg gatcgacgtg aaggagggac cgctgtttgg gacagaggta gtcccttcgg
    12301 caagaattga cgaattcctc aaagtgaaaa ggcccgatgt cctgatagac ttcaccgttg
    12361 cagccgctgc ggtcgagaac atcaaggcgg cagccagaaa caacgtggcg ctcgtcgtcg
    12421 ggacgaccgg gttctccccc gaacagaggg aagtgatccg gggcgccgtc gaagggcatg
    12481 tccccgcggt gatatcgagc aacttctcgg tcggcgtcaa catcttctgg aaactcgtgc
    12541 gggaagccgc ccgccagctc ggcgactacg atatcgaggt cacggaggcc caccaccgct
    12601 acaagaagga cgccccgagc ggcacggcaa agaccatcct tgagatcctt gaccaggaac
    12661 tcgggaaccg cgagaaggtt tacggccgcg tcggtgagac ggaacggaaa ggcgagatcg
    12721 gtgttcacgc catccgtggt ggcgacattg tcggcgatca ctccgtcctc ttcgcgggga
    12781 acttcgagtg tatcgagatc tcccaccgcg cctacgaccg ggcggtcttc gcgcagggcg
    12841 cggtccgggc cgcacgctgg atcaagggcc gggaaccgcg catgtacacg atgcaggaag
    12901 tcctcgggct ataaaaatgc tcccgatcca tcccggggag cgaaatttat ttttgatgca
    12961 ctcatacact aatgtaagtt cggaggaaca aaagtgccag cagttgttga tgtagagaag
    13021 tgtaccgcct gtgagacctg cgtggacgtc tgtcccgctg ccgccattca tctggaagac
    13081 ggcaaagcta aagtcgacgt cgacctctgc gtcgattgcg agacctgcgt cgacgagtgc
    13141 ccgtcggaag caatctcgat ggagtgaact accccgccct gaag
//
LOCUS       DAJB01000034            6797 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_15859, whole genome shotgun sequence.
ACCESSION   DAJB01000034 DAJB01000000
VERSION     DAJB01000034.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_15859"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1242 a         2196 c         2115 g         1239 t
ORIGIN      
        1 gcttgcgcag ggggtcttcc cgctctgccc cgcgagcccg gcagagataa atgagtccgg
       61 aaagggaaac tgggcatacc cgggaatggg gttcaagcac cgaaggtggg ggttacgcgg
      121 tcccccacga ttcacttacg gaacaagccc gggaaaaacc ggggctggca taccggtgtc
      181 ctgcagcaag cgcatcggtc tcgcatcgat atctggagat gacctccagg atagacgtga
      241 tgagccctac gttaccacca cgacaggaac agggctttaa gcaaatcctt ttatggctcc
      301 tgtaaatgtt atatcattta catttacata aaattaaata aatacaatgt acaggtacgg
      361 tatggactgg tgcacaggat gacctgtggg aaagaaccga ccgcggagac cgggcaggaa
      421 cccgggaaga acgttacact cgacctgccg tgggttacct cccgcggcgg catgccggtt
      481 cacaagaccg ttattcagtt tcttccggta aataaggtga tttacctcct cccaatcgcc
      541 ctcttcttct tctccctctt cttcgggagg tacatgatgg acccggtgga aaccctccgg
      601 atcctcgttg tcggatcggc aaacatgctc cttgacggtg tgtcgtccct gctctcgacc
      661 ctcaccggcc atcaggtcct cctccctttc ttcgagcaga cctggggggc ttcggagcag
      721 acggtcgtct tccagatcag gcttccccgg gtgatagcgg caatgcttgt cgggggagga
      781 ctcgccctcg ccggcgcctc gttccagggc ctcttccgaa accccctcgt ctcgcccgac
      841 atcctcggcg tggccgccgg cgccgggttc ggagcggcgc tcgggatcct cctctccggc
      901 aacccctggg ttgttcaggt ctctgccttc gtcttcggga tcgtcgcggt cgccgtcact
      961 tacggcatcg ggcgcatcta ccacaactcc tcgaccctcg tcctcgtcct cgcggggatc
     1021 atcgtcgggt cgctcttctc cgcccttctt gccctcacca agtacgtcgc cgacccgtac
     1081 gacaccctgc ctgcaatcgt cttctggctg atgggaagcc tctccgcggt ctcgaacgct
     1141 gacataatcg cggtcgcccc gccgatcctc ctcgggagtc tctgcctctt cctcatcaga
     1201 tggcggatca acctcctctc ggtcggcgaa gaggaggcgc aggcactcgg gctggacacg
     1261 aagaagatga ccgtggtgct catcgtcgcc tcgaccgtcg tcaccgcctc tgcggtctgc
     1321 atcagcggga tcgtcggctg ggtcggcctc gtcgtccccc acatagggag gatgctcgtc
     1381 ggccccgatt ttaagaagct catccccgta tcggccgttc tcgggggctc atttctcctg
     1441 atcgtcgacg atatcgcaag aaccctgact gcaaccgaga tccccctcgg catcctgacg
     1501 gcgctcatcg gcgcgccgtt cttcgcatat ctcttaacgc aaaagaaggt cggctggaca
     1561 tgatcctgaa cgtcaagtgc gcgtcgttct cgtacgacgg catcacaaaa cagttcgaag
     1621 atatctccct ctccctgcgg agaggagagg tgctctccct cctcgggagg aacggcacgg
     1681 gaaaatcgac cctgatcaag tgcatcttaa acattctcgc gctccggagc ggcgaggtgg
     1741 agatcaacgg gcgccgggtc accggcatgc ggcccgacga gatcgcgcgg gaggtcggct
     1801 acgtccctca ggtccaccac gccgtcttcc cgttcacggc gctcgacttc gtcctcatgg
     1861 gaagagcccc gcatatcccg accttctcgg tcccgaaaga ggaggactac gagaaggcgg
     1921 aggaggcgtt tgcgcggatc gggatcaccc atctccggga gaagtgtatc tccgagatgt
     1981 cgggcgggga gtcgcagatg gtgatgattg cccgcgccct cgctcaggag ccgtcgttgc
     2041 tcatcctcga cgaaccgacg tcgcacctcg atctcggcaa ccagatgaag gtgatcgcga
     2101 cgatagaccg gctcgccgcc gacgggattg ccatcctgat gagcacccac ttcccggacc
     2161 acgggttcat ggtctcccat tccgtagcca tcctgcagga cggcaggctg atcgcgcagg
     2221 ggccggccga ggacgtcatc acgaaggaga acctgcagga agcctacggg gtcgatgtct
     2281 gcgtctgcta cgtcaaagag gcagaacgga tggtctgcat cccgataagt ccgtgcggtt
     2341 gccgcggcag ggaagaggtg taatggtccc gatacggcct gccatgatcg ccttctgcct
     2401 cgtcgccgca gagctgtccg gtaacctggg cggtgacggg tgcgaccggc gcggagggcg
     2461 tgctcccaca tgtccgtgag ggggatgggg agatcgcctg accgcgcccg ccgcctaagg
     2521 gcggtagaac gatcggtttc cggtggtccc ggccgtaccg gtcttataaa acgtccggta
     2581 gaaccggtcc tgctccgcag caaggtcgaa cacagcacgc tccgggtgga ggatgccggc
     2641 gatggcggag aggccgagaa cccagcgggg gttgccgaag tcccagcccg gcgggagggt
     2701 gtagacccgg ccgtgctcga ccgcgtcggc agagagcccc atcctccggc atgccgcaag
     2761 gtaatcggag gcgggtgcgg agaggaaccc cgatatgaag atgtactcgg ggttaaaggc
     2821 ggcaaactct tcaggcgtta tgttgacgcc cggttttccg gcccggagaa tcccacggtt
     2881 gacggggtcg ccgccggccg cctccacgag gttcatctcg aaccgttcgg cgttcagggc
     2941 gaagagcggc gtccccatcg agtagtagac ccggggccgg cggcagccct cgaccgccga
     3001 cgagaccgcc gacacccggt cattgatgta ggtggcgagt cggtccgctg ccccggggcg
     3061 gccggcccgc tcgcccagtt cccggatgag gtcgagataa ccgtctatcc gctggatctt
     3121 cccgtggagg tcccggatgt agtcatcccg aatgaccccg gcccagatac gggcgaggtc
     3181 ggcatcggac tccacgccga ggcagaccag gacggcgtgg accatctcca gcgcccgggt
     3241 gaaccggtaa cctcccatca tgccgggctc ggcataggat gtctccccga cactcccggt
     3301 cacgggcagc ggaaacccgc aggagcaccg ggcatccggc gggatcgtga tcgtccgcgc
     3361 ccccatcggc ccgaaaaatt cccgccgcac gaccgtctcc ccgcaggccg ggcacgccgt
     3421 atcgagatat ccggtcccgg gggagttgaa gagataaacg tagcggagcg tccgccgcag
     3481 gtcgtcgcag attcgctccg actcagcaat cgtcggttcc agcgccggcg actcgtcgcc
     3541 gaacgggatg aaccgcatga cgaggaacgg aatatctggg gatatctcca caatccgggc
     3601 ggccgcggca cggatctcgt cgtcacggcc ccgggcatgg atgcaggata cctcgacatg
     3661 gacacctgct tccagaaggg tcttcaccgt ccgaaaggcc ggttccgccg aaaccgcacc
     3721 gcatgcgcgg taacgctcat cggacgctcc cttcagaccg atgtttgcga aatcgatggc
     3781 gggtgcaagg tcacggaggg cttccccggt atagtagccg ttcgtcgcgc acccgacgag
     3841 gaggcccgcg tcgtgcgccg cacgggccac ccgcaggaag gtctggtgca tggcggtcgg
     3901 gtcgttgagg cagaacgcta tcccccggca cccctccttg agggcgcgcc cgaccagagc
     3961 gtcgggagcg atatgcctga gggccccgga gacggcatcc gcgtggaggg cgaccgtctc
     4021 ggatatgcac ccaccgcagg agaaggtgca tccgatgccg cttgcctgca aaaacgtccc
     4081 gcgggggtgg taatgacaca tcggcatcgt ctcgatggcg atcggcatga ccgcgatgta
     4141 atggtcgggg aaccgctcga cgagcttccc atcctcgttt gtatacatcc tgcaccgtcc
     4201 gactcctccg ggcgggacga cgcaacggtt ctcgcagatt gagcagcgca acacctaacg
     4261 cccccgctcg atcacgaccc agaaccctga gtcgtcgctg atgatccggt gacggaaccc
     4321 gaggtctgcg aggatcccgg cgatccgctc ccggttttcc aggccgaggt tcctgtcccg
     4381 gaaactcctc cagtccgggt tctccctcgc catcgtggcc gcgacggcgt tccggaggtc
     4441 ggcagtcccg aacccgcccc cgacgtaggt cctcccgccg ggtgcgagaa cccgatggat
     4501 ctcggagaac gcgcgggcga gatcctccca gaagaagatc gacccgcggc tgaccgcgag
     4561 atcgacggac ccgtcgggaa gcgggatctc gtggacgtca ccttcaagga gcgcgacgcg
     4621 ggccgaaagc ccggcctccc ggacgttccc ttcggcaata gcaatcatct ccagggagga
     4681 gtcgaggagc gtgaccgcaa ggtcgctcgc ctgcgcaagc gcgatcccga gcgatccggg
     4741 accgctgccg acatcgaggc accgtccgca ggagatcccg caccgctcga ttacctgttg
     4801 cgcgatcacc ggatagatcg gggcgaagac agtctttgca attcggttca tatcctctgc
     4861 cccttcgcgg tcgaacctgc ctggtacgta tcccatgctc tcggcctcag gggttcagga
     4921 catcccggat ctggtcgtcg gaaagcgata cgtggataaa ggtctcgtga tactcacgga
     4981 cgagggattc nnnnnggcgg gcggtcgaac cagcagaacg ccgtctgcgg gatgagatag
     5041 acccggtcgg tcttcacggc ggcgatctcc tgccagagcg gatcggtccg gaccgtggcg
     5101 tagaacccgg ggtcgtggac cagaatgacg tccgggtccc aggcgatgac ctgctccatc
     5161 gagaccttcg tcatccccat ccccggcgtg atcgcgcatt cggcaacgtt tctcccgccg
     5221 cagaggtcga tgagctcggc gtgctgcgag cccgaggggt cggtcatgag ccctttcggt
     5281 ccctcggcat agtagacccc cacccgctcg tcgtcgggga tcgaggccac ccgctccgtc
     5341 accgtggcga gcacgccctc gtagaaggcg atcatcgctt cggcctgctc ctcttccccg
     5401 aggagatcgc ccatgaaccc gatgggtgcg gagtagccgg tggcgttggt gctgtcctcg
     5461 acggcgacga ccgggatgga gcccagcttc tgctgccgct cggcgaccga ggcaagcgcc
     5521 gccccgccgt cggtggtgta gccttcgacg acgatgtcgg ggtgcatcga gatgaaggtc
     5581 tcgtagttgc cggtctcttt cccgaaccag cccccgatga ccgggagcgc catataccgc
     5641 tcggggatgt acccctccac gggctggaag ttccagcctg cgagccggtc gggcgcgacc
     5701 atgtagacaa gcatcgtcga cggcggcgag gtgcagagga cggtctcgat ccccgacggg
     5761 acgacgacgg tccggcccgt catatcggcg atcgtgcggt tttcagccgg cgaaaccgtg
     5821 ctctgggtgg ccgtgcaggc ggcggaagcg cagaggagaa ctgcacagac accgagaatg
     5881 atgagcagaa gatgacagtg ttttgtaata actaccacgt ccattcggtc ctctcggtac
     5941 gctccaggga ggtatcggag gtttatggta ccatggttgt ggcgtgggcc ttatttaaga
     6001 taactattta actttatgtt aatgttaata attatacatt gaaattacat tgaaaaactc
     6061 tgccctgatc cggcggtggg gatccgggac gtgccagtgc tcttcggcct cccagggtcc
     6121 ctccggccac agaatcccgg tccacggttt actgtgaagg acatctcgca atgagaaaca
     6181 gagtcccgtt cgtcatcaca tccgcgggca cggcttctcc cggttgtcgc cccgcggagg
     6241 gcgactggcc ggatgggttt tgagctgcac cggtaaacct gcactctccg gggggcaagc
     6301 ccgacagaaa ttcactcact cctgccccgg atcgcttcga taaccacgcc cacaagccgc
     6361 tcgatctcgt caaaccgcgg ctccgccatg ccgttcttca cgatcccgca cccggtcgcg
     6421 acgacgtgca ggtccggctc gaccccgatc tcacgaagct ttttgagcgc acaggtgtct
     6481 tcgcagccgt ccagcgcgag gaccctgtcg acgagcaggg acggcaggcg gcttgccggg
     6541 atgcaggcct ccacggattc cggagagcgt tgcaggatca ccatacccgc cctggcggtc
     6601 agcttcccgg tattcgagag gcccgagcag gtgacaaggg tgacgggctc cgtcatggcc
     6661 tcactccatc tcaagggctt ttacggccgc ccgcccgatc agtaccggcg ggatcgtctc
     6721 gtactcctcg ccgttgtagc ggagcgcccg gcactccgtg ttttcctcgc aggtgagaca
     6781 ggagcaggag gagcagg
//
LOCUS       DAJB01000035            6717 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_103364, whole genome shotgun sequence.
ACCESSION   DAJB01000035 DAJB01000000
VERSION     DAJB01000035.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_103364"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1210 a         2170 c         2130 g         1205 t
ORIGIN      
        1 acgttccaga gttcttcaac taccgcgaaa gccagctcaa agaccttgcc taccaggtcc
       61 gtcccgcact cgaagggggg ggcgctgaac gccgtctgca ggggtctgcc cggaaccggg
      121 aagacgacca gcgtgctccg gatctttgcc gaactcgagc agaccacaaa gaagatcctg
      181 ccggtctacg tcaactgcca gaacgaccgg acgaagtaca tggtcttctc ccggatttac
      241 gcagcgatcg tcggccatgc tccggcgcgg accgggatct cgattaagtc ggtgatggac
      301 gcgatcggcg acgccctgca gcggcgggag ataagcctga tcgtctgcct ggacgacgcg
      361 aacctgctcc actacaacaa tacgctcggg gatgtcgtca actccctgct ccggctgcac
      421 caggactatc ccggtgcccg ggcggcggtc tttgcgacgg tcagcgacat ggaccttgac
      481 cttgcgagtg aacccgggat gcggtcacgc cggttggtat agccgtttcg atcacctcct
      541 gtccggtccc gctatagcgg gaacctattt gaccatatcc ccatgagcag gattgcccgg
      601 ctgcccgccg gtacaagact tataataggg gagagtccca gaggggcgca cccgatccag
      661 gggagatgag agatgatcca cgcacgggga agcggcatac tcctgcatat cacgtccctg
      721 ccgtcggcgt acggcatcgg cgacttcggc ccgtcggcat accggttcgt cgaggcgctt
      781 gagcgagccc ggcagcacta ctggcaggtc ctgccgctca acccgacatg cacggcgtgc
      841 ggcagctcgc cctacagcag cccgtcggcg tttgcgatga acactctcct gatcagcccg
      901 gagatgcttg ttcgggaagg ggttctcagg aaggacgacc tcgaaccggc gcctgatttt
      961 ccggaggagc gggtggcgta cgaggcggct gcctggtacc gggaacggct ctttgcgctt
     1021 gcgtacgagc gatttcgcca ctcgggaccg gagcgggagt ttgagacgtt ctgcgacgcg
     1081 agcgagtggt ggctcgacga ccacgcgctc tttgtcgcgc tcaaggacca ctttcacggg
     1141 ctcgcgtgga acaggtggcc ggaggagatc cgggcccggc atccggaggc cctcgacgat
     1201 atgcgccgga gcctcgccga taaggtccgg aaagagacgt acctccagta cctcgccgcc
     1261 cggcagtggt cggccctccg ccgctactgc accgaccggg gcatccaggt catcggcgat
     1321 atcccgatct atgtcgcgta tgacagcgtc gacgtctggg ccaaccccgg gatctttcaa
     1381 ctggacgaag atctccgccc caccgttgtg gcgggcgtgc ctcccgatat gttcagcagg
     1441 accgggcaac tctgggggaa cccggtctac gactggcccg cgctccggga gcggggctac
     1501 gactggtgga tgagccggat agcccggtcc gctgaactct acgacctctt ccggatcgac
     1561 cacttccggg cgttcgccga ctactacgag gtcccggcgg gcgatacgac ggccgagcac
     1621 ggcacctggg tcgacgggcc gggggccggg ttcttcgagg cgctctcccg ccggttcccc
     1681 tgctttgcca tcatcgccga ggacctgggg gcgaacaccc cggccgtcca ggaactcctg
     1741 gaccggttcg atttcccggg gatgaagatc ctcctcttcg cgttcgggga agggatcgca
     1801 aggagccccc atatccccca taactacgtc cataacctca tctgctacac ggggacgcac
     1861 gacaacaaca cggcccgggg gtggtttgag gaggaggcgt cagtcgagga taagcagcgg
     1921 ttctttgctt acgtcgggcg ggaggtggcg gcggacgagg tccaccggga actcactcgc
     1981 ctggcgatgg cctcggttgc ccgggtaagc gtcatcccca tgcaggacct cctcggcctc
     2041 ggcgccgcgg cgcggatgaa ctacccctcg acggcggaag ggaactgggt gtggcggatg
     2101 acgccggcgg agttttccgg tgccccgttc gactggctcc gcaggatgac ggaactatcg
     2161 gggcgggggt gaatcagtac cgggtcaacg cctcgagttc ttccctgatg gtccggcgga
     2221 ccatctcctc gatggcggtg tagtcccgcc gggccttctc ctgcttagcg atcatctctt
     2281 ccatctgcat gatcccgaga gagaggttct tcccgtactt gagggcgacc tccagcgcct
     2341 cctcgtcgac ccggacgact ctggacatat aaggatattt tccgggccgg ggtttaagtg
     2401 ttacagtttt gttacaggaa agtggcgatt gttacaaaac agtaacaatg ccggagaggg
     2461 tgtaacatct gtgttacaga agcaatcgcc aggacctccg gggggggaat cccgggaatc
     2521 tcggagatgc gatatccccg gctatttcac ccctgcctgc cgggccacgc cctgtgcggc
     2581 cgctgcgacc tcctcggggt cgcccagata gtagtgcctt acagcccgca ggtccccgtc
     2641 cagttcgtag acgagcgggt agccggtggg gatgttgaga accgcgatct cgtcgtcggg
     2701 ggtcccgtcc aggtgcttga ccagggcacg aagactgttt ccgtgggcag cgaccaggac
     2761 ggttttgccc tgccggagat cttctgtgat atggctctcc cagtagggga gcacccgctc
     2821 gagcgtgtcg tgcagggact cggtcgcggg aaggctctcc gggtccaggt ccgcgtagcg
     2881 ggggtcgaac cgcgggtgtc tttggtcgtc ccacgagagc ggcgggggct gcacgtcgta
     2941 tgcccggcgc cagaggtgca cctgctccgc accgtatttc tcggcggtct ccttcttatt
     3001 caggccctgc agggcgccgt aactcttctc gtttaaccgc cacgaccggt gcaccgggac
     3061 gtacatgaga tcgagatcgt ccatgacgat ccagagcgtc cggatggccc gcttcaggac
     3121 ggaggtgtag gcgacgccga acgtataccc gccttcctta aggagttccg ccgcccgatg
     3181 ggcttcttct atgccgcgag gtgagaggtc cacgtctgtc cagcccgtga accgattctc
     3241 actgttccag aggctctcgc cgtgcctcag caggatcaac gctaccattg caattttact
     3301 ctcacatcca gtatgatcct cctccacgaa taaatgccat gcacgtaccg ggggtagcct
     3361 tgcaagagtg gccggaggag ccgcccgtgc agccccggcg gggacacggg gcaacagaaa
     3421 cctcgattcc ctctagaatc atcagatttg acgaacaggg gatgtatggg ttcgattttt
     3481 ttcgtagcct acaattcata tggcaccgaa cagaagaagg aacagaaact gcatcctcgc
     3541 gatggcgtca ctgctcgtca tcgttgtggc ggcagtcatc accgcaggat gtacggcggg
     3601 tacgtcgacg cccggcgcgg ttgccgggcc ctcctggact ctcgactcgt acctcgccgg
     3661 gaacggcacg cttgttccgg tcctgccggg aacggagatc tccgcccggt tcgacgatga
     3721 cggcaaggtc accggctccg ccgggtgcaa cggttacggt ggcgactaca acctcgacgg
     3781 cacgaccctc tcgatctcct cgcttgtcca gacgctgaaa ctctgcacgg agcccgaagg
     3841 catcatggag caggaggcgc agttcatgga cctcctcggc tcggcggcag aatgccggat
     3901 cgaggatggc cggctggaga tcatcgacgc tgccggcacg acgacgctcg tcttcgtgaa
     3961 gggacacgcg gccgcggcac tcgccgccgg gacgtcctgg acgctcgcaa gcctcgccgg
     4021 ggaggacggc gctctgatac cggtccttga cggcacaacc gtcacggcgg ccttcgacgc
     4081 cgacggcaga gtcggcgggt cggccggatg caaccattac agcgcagact acaccgtgga
     4141 cgccgcgaac ctcagcatcg ggccggccgt caggaccgag atgtactgca gcgaaccgcc
     4201 gggcatcatg aagcaggagg gccgctacct cgcgctcctc gcgaacgtca cctcctgccg
     4261 ggtggaagga ggcgaacggc tggtcctcgt ggaccgcgag ggtgccgacc tgctcgtctt
     4321 tgaagaggtc gtccggacgc ccgaccttcc gttcgtcggg acggactggg tgctcgagtc
     4381 ctacagcacc ggtggggatg cagtctcttc gcccgtcaac ggtacgaaga tcaccgcgaa
     4441 ttttgccgac ggaaacgtca ccgggaacgc cgggtgcaac cactacggcg gcgagtacag
     4501 cctcgacggc gcaaacctct cgatctcctc gctctacagc accctgatgt actgcgagac
     4561 accgggcgtc atggagcagg aagggaggtt catgggccac cttgcaaacg tctcctcctg
     4621 ccgggtggag agcgaccgcc tggtcctcac ggacgagacg gacaccgacc tgctcttctt
     4681 cgtgcaggca ccggcggtgc cgccggcacc gctcgccggg acggaatgga cgcttgagtc
     4741 ctacagccag gacggggaga cggtctcctc gctgatcgtc gggaccacag tcaccgcgac
     4801 attctccccg gagggcaagg tcaccgggag cgccgggtgc aacgcctacg gggccggcta
     4861 cctgctcgac ggcgcgaaga tcaccatcnn atcagcacga agatgaactg caacgagccc
     4921 cagggcgtca tggggcagga gaacacgtat ctcaacctcc tcccaagcgt ctcctcctat
     4981 cgggtggagg gcaaccatct ggtcctctcc gacgcgacgg gcgccgacct gctcttcttt
     5041 atgcaggcac cgctcgacga ggcccggtag atctccccgc ctgccgggga ccagtacggc
     5101 ctcctcgatc aggacggaaa tgtcgtcgcc tcatactgat tggtgagccc ggatcggtcc
     5161 cgatcgatcc gccctcccct gcccgatctt ttctcatttt atcatacccg gacggcctta
     5221 tcgcccgccg gggcaggctc cgggcggcag cggcggatcg acgccccatc catccggaca
     5281 cgggggagag ggggattgag ataatctcaa cgcaaatagg ttattttttc acctctggct
     5341 ccctatgcct atccgtgata gagatgggga gagacggaca ccacgcattc gcaaaggccg
     5401 cattattgat catcgtggcg gcatgcctcg tctcggccgg ctactccggc aggacggaga
     5461 cgcctcccgg cgatgccgac cgggtcaccg aaccgcttgc ggggacggca tggcacctgg
     5521 ccgcgtttcg tgccgataac ggctcggtca tcgcgcccct gcccggggcg gagccgctca
     5581 tcatcttcgg cgagggcggc acgctctcgg ggtccgccgg gtgcaacctc tactccgctc
     5641 cctaccggat caacggctcc acgatctcgg tggagccggt catcgccacc gcggcctacc
     5701 ctgcgacagc acaggagctc gcgcagcagg agagccggta ccaggaactc ctcgttgcag
     5761 ctgcatccta ccgggtcgag ggcgaccgga tgacgatctc cgggccgtcg ggtaaggacc
     5821 tgctcgtctt ttcacgtgca gagcagcctc aaagcatgcc gctgctcgcg acagaatggc
     5881 aactgacccg ctacaccacc ggcgggggca acaccgtcgc gtcgccgatc cccggcacca
     5941 gcgttaacct gacgttcgat gccgacggca cgctctcggg gtccgccggg tgcaacgcct
     6001 actcggcccc gtacgaactc aacgagacgg ggatcgccgt ggagcgggtc atcgcgacga
     6061 aagcctccct cgccgaaccg gcggggatca tggagcagga gagcgcctac ctggacctgc
     6121 tccggtcggc ggcgggttac cggatcgtcg gggatacgct cgcggtcatc gacggcaacg
     6181 gcagggcgat actgttcttt aaagcagaat cgtaagggga atccccggcc gaagcgctct
     6241 tttttttgca ggcgggcgac gcacacaaca gtgcttctgc tcctgccgga atacgactgg
     6301 ctgcaggtgc gacgttccaa cagaacagca ggagcactgc aggtgcgacc tgcgatgggc
     6361 cggggggacg gggcttgaga aaccgccagg cttcgagcag gagttcaagc accattagat
     6421 ccgagttgga gacagggggc ggggcgagcc ccctccccat cagccccacc cctatggcga
     6481 tactcccccg gcccactgca cggggatttt tctcaatttg gttgctttgt ttgtcgcaac
     6541 tcgcaccttc gtccgtcgct tatcgccacg cactgccccc gctcccgggc gcggtggggg
     6601 agtcatcagg acaccaaccg ggtttcaaca gagcaaaagt gaactacccc ccgctttcgc
     6661 agggggcttc ctgcgagtgt ccggggctct ctgggagcct cagacctgtt tgtcgca
//
LOCUS       DAJB01000036           13267 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_34024, whole genome shotgun sequence.
ACCESSION   DAJB01000036 DAJB01000000
VERSION     DAJB01000036.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13267)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_34024"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2460 a         4072 c         4044 g         2689 t
ORIGIN      
        1 ctcccgcggg gcgaacgtgt acggggcctg atgcgggcaa ttcctccccc ggttgaaacc
       61 gggggcctcc ttgcccgtcg tatatgaacc gtctcccgga aagccaaacc tacaggtcgg
      121 aatgaacctt acagcgtggt ctcagatttg ttttataagt ttagtaatga tttttcatga
      181 caaatccctc gggggatgct gcgtgccttt ccggattctg ccgatctgca tccggatcgt
      241 tagaaccata gagcagttgc ccccgttccc ggttgccggg ggagtgtcct cccgtgggtg
      301 cgggatgtgg ggtccgcagt attcgccggg atctgccggc tgggggttgg gctatgctcg
      361 tggtctgctc cgaaggagtg ccgcctgtaa ggtcatccgg gaaccttctg tcgggggtgt
      421 gaagcccgct tacaatcctg tccctgaacc gatttatatg gcggctccct cgttccggga
      481 atctcccggg atcccgggcg aaaggagagc ggatgcgtgg ggtcgagcga cgcacgatcc
      541 gtcgtcgtat ccggaaaatt cttccccgat cccgtcgaca gttgtaactc cctggattac
      601 atgagaaccc gggagaagaa tcatgatcgg gcatcactcc gcaccggatc gccggacgat
      661 gtatattctc ttcatggtcg gtttctttgc aatcttctcg accaccatct caaagaatcc
      721 ggtgctgccc ctcttctcgc aggccctggg cgccggcgat gcagtcatcg gcctggttgc
      781 agccgtctcc ccgctcgccg ggatcctctt ctcgttcccg gtgggggttc tctcggacca
      841 cctgggcaag aggagactgc tcatcgtatc cggagcgata ttcctctttg cacctctgct
      901 ctacctcttc atcgccgatc cgctctggct gatcccggtc cggttcttcc acgggatggc
      961 aaccgcaatc ctcggtccgg tgatcgctgc gatgattgcg gtgcgatttg ccgagaagaa
     1021 gggggaggtg ctcgggcagt actcttccgc aaccctgatc gggaggaccc ttgccccgct
     1081 tgtcggtggg gtcatcatat cgttcttcgt cttttacccc gggctcattc cttaccggat
     1141 ggtatacctc gctgcagcaa tcgccgcggt gccggtagtc gtcctgatcc ttctctaccg
     1201 ggaggagact ccgggccccc tcatggtcct cccgttctca gcgtttcgca gaagtttcgc
     1261 gacgttcttc gcgaaccgga gactgcgggc aacggcactc gtggatatgg caacttactt
     1321 tgccttcggg gcgttcgaga cgttcctgcc gctcatactg ctctcccggg ggatgggtgc
     1381 ataccagacg ggggtcctgt ttgccgtcca gacactgatc atcgcggtga caaaaccatt
     1441 cttcggcagg attgccgacc gggtcgacaa acgggtccag attatcatag ggctcttcgt
     1501 tatggggtgc tcggttgccg ctataccgtt tgcgtcggca tttacaacac tccttctcgt
     1561 aagttccctt ctcgctctcg gcatgtcgct tgccacggtt gcgaccactg cgtacgtcgc
     1621 agacgttgca gagaaagaag agatgggcgc ctcgatgggg gcattatcgt ccatcatgga
     1681 catcgggcat tcagcaggcc ccctcgtgac tggaatcgtc attgccggta gtggctttgg
     1741 ccccggattc ttcgtgagtt tcctgatcgc cgcagcagtc tgcgggttct tcgccctgtc
     1801 tgtccggaac ccggcaccgc agacgggagc cgataccgtt agcgagtaaa ctactccgcc
     1861 cgcgggtccc taatgcgtcc tgaccttcag ccccggaacg cgggtgaagt gactgtcccg
     1921 ggtgacgatc tcgccgtcgc ccgtgagtgt gatcgcggcg atcacctcgt cgaaatcgcc
     1981 gataggtctg ccgtttcccc tgagttcggc agcgagcgtc ccaaagaccc gataggtctc
     2041 gtcggtgatc gggagctcga tcagcgcccg caagatcgcc tcgacttccc ggagattttc
     2101 ctgcaccgat gccgagagga acgcaccgcg gtagagttcg agggcgttga tcgccgtggt
     2161 tgcaagcggc agcccttcct gctccatcgc ctcgagtgtt ctcatcgcct ccgggtcgtg
     2221 ccggatcagg tcgatgagca gggatgtatc aagcgtcggc atcgaagtcg acctctcgca
     2281 tcgaagattt ccgcatttca cgcgaggctt ccgcaaccga atcggccagc gcggggttcg
     2341 gcccgaaccc tttgaggatc tccgagagtt tcttcttttg cggggtgtac ttcaggatca
     2401 cttcggaaaa actcatgttc gaggggcctt ttagccggga aagcctctcg tatgcgtcgt
     2461 cactgatact gatcgtcctg gttcccatac ctgtgtatgt gtatgtgcat gtattaagat
     2521 cctcccgtcg caaggaagac gcttcagaac ttcaccagcc cccgcctccc gatcgccgtc
     2581 caccctaaaa gacacgccac cccggccgcc acgttgatcg ccccgaggcc gaagacgagg
     2641 gagttcccga agaggaagac gaggaccatt ccggcgcttc cgaagacgag gctcgagcag
     2701 ttgatcaggg tggctgcgga gccggtatcc tctctctgct ggtcgagcat caggaacgca
     2761 cccgccggcc gcacggcgct ccccatcagc gaggccggga agagggcaag ggcaaaggcc
     2821 agggggccga agttcccgaa gaggcagacg agcaccccgg cgccgatcat gacgacgaac
     2881 ccggctccga cgatcgagcg gcggcttgcg tgccgggaga gccagaggta gaggaccggg
     2941 cccgctatca ggccgacggc gttgagggcg aagtagaagc tgtaccaccg ctccgagagg
     3001 nngcggtgct cacgagcgag aatacgacga ggagcgaggc gaaaccgggg tttttgagca
     3061 cgaccccgag ccggcgggcg gactgcgcca tggtgccggt cgaccgcgag ggtatggtct
     3121 cttcgaggag gatgccgccg acgaacgaga cgacgccgat gagggcgagc gtcacgaaga
     3181 ggccccgcca cgatgtgtag gggagcatga acgccccgag aaccggggcg acggcgggcg
     3241 aaatgacgac catggactgg acgatcgcga gcaccgattc ccgtctcctc ccgtcgaaga
     3301 cgtctttgac catcgccatc gcgaccgcgg cggcggcgct gccgccgacg gcctggagga
     3361 tgcggaaggc gatgaggcac cagatatccc acgatacggc gcaaccagcg cttgcggcga
     3421 tgtaaagggt aagtccgacc aggaggacgg gccggcggcc gtaccgatca gagagcggcc
     3481 cccagaagag cagcccgagg ctgaagaaga ggaagaagag tatcagggtc aggttggtga
     3541 gggtcgcgga gacgccgaag aagtcgccca tgccggggag ggcggggagg tagagatcgg
     3601 tcgagagcgg gacgaaggcg gagagcagcg cgataaggac gataagaccg cgatccccga
     3661 gatatcgccg ggttgtccgg tcattgtcgg gggattggtc catggaatcg tctcatgagg
     3721 caggtgttga agaacaggat gccgctgttg tgcattcctg acagaagccg gattcttctc
     3781 ttctccaacc tctgtttata cattgaacct tcccgtagtt ctagattggc attgggcgtt
     3841 atcctgcgcc tgcaggtcga tatcttcgat ccgtgcctgc tcagagcgat gagcaatata
     3901 actctggagg cgattcggcc cgtgagggat tcaacagagc cagaaaagta aaattagttg
     3961 gatattccag gcggctcact tgagtttttt ctcgagtttc ttgagcttct tcttggcggc
     4021 ctggctgccc gcagccgcct gctgcgagag ttcttctgcc ttggccttct tcactttgtt
     4081 taacttctcc atttgtttct tacttgttgg cattacaatt acctcgctac gcctttggct
     4141 ggttctattt actttttttc cttcttataa atatccctgt atcgttgtac tggcccggaa
     4201 catcttcgaa taaacgaaaa actgacctat tcaactgctg ttttacccgt caatatcttc
     4261 ccgcccgtaa tgcatcgtaa agagcgtcgt gtgttcgtcc gcatagcagg gtgagtggca
     4321 gcagggagtg aactccggac ccaggcattc cgcaaagagc gcatcccact ggccctgcag
     4381 catatgataa tcggtccgct caccgcgggg ttgcgtgaag aacgagatga cctccgtcat
     4441 caggaagaga tccacgcagg cgacggctac gtccaccggg gcgtcgtgcg agaagacgaa
     4501 atcgatgaca cccccgtagg actggtagac cgagagcgca agttcagggg aatacatctc
     4561 cgggacgtcg ttgttcgcat ccatctcgaa gaggacgaag tgatcgatcc agggtttgag
     4621 ttctgtgagg tggatcttct tatgctcgat gggcatgtgg tggtaggcaa gcgagctgac
     4681 ggcgagatcg aaacggtgat cgatgctgcc ggaacagtcc tggatccggc tgttcacggt
     4741 ctcgatacgg atatcgggga atgcatcgcg gacggtcttt tcagcaaggg ccaccattgc
     4801 aggcgacgaa tcgatgagga ggacttcggc aatctcagtg accctgccgg tgtcttgcag
     4861 gtgccgcaag agtgatacgg tgagccctcc gtcgccgcac cctatgtcca tcatggcaaa
     4921 cggcccatcg atctgcggca gggtgttgcc gcactgtgca atgaatcgct ttcttgcctc
     4981 ccgcatcatg gggacaccgg caaagtgcat gaacgggcgc gggtcggcaa acgccaccgt
     5041 gggcatggcg agtttcccgt tgtggcgctc aaggaagccg aggagaaagc ccgcagagaa
     5101 gagatcgctc tcctcgccaa gagcagcggc gttcagccat tcgatcccct ctgcggcctg
     5161 cccgtgggcg gcaagcactg ttcctatgta gtagtcgaag gcagagagca gattcggccg
     5221 gatgtcgccg tcataggtgc ccggcgcagt cccgctcctg agaacctcgt ctgcaaggcc
     5281 ggaccactcc gccgactcct gcgctgaaag gaagagcata ctgatcgtat tctgccggca
     5341 accggtatga gcgttcgtat tctctcccgg tcttatgggc aggattcatg atctggacgg
     5401 accgcgagcg gttgtcgata atgccggata ccacaagcaa aagaacatcg tatcggatta
     5461 aaacttcaaa acagtcctga aaggatgctg ctaaggggat tcgaacccct gtcgagggag
     5521 tgagagtccc tcatgattgg ccggtctaca ctatagcagc gcattgcatc aacgtgctct
     5581 gcattattgg gcataattct atttaaatct gtctcacgcc ctcccctctc cggtcctgcc
     5641 ggtaacagga caatcggggc cctatacggg ccaggatttc ctctatgctt cgtgatttcc
     5701 atcataggta ccaataagta gcttcccagc atacatgtac cggcagcaat gagcagtgtc
     5761 gaagagcaga tacgggaact tggggacgaa ctcaaaaata ccccgtacaa caaggcgacc
     5821 tccaagcaca tcggtcgcct caaggcgaaa ctcgcgaaaa ttcgtgacga agcggtaacc
     5881 cgggccatgg cctcctccgg agggggcgag gggttctccg tgaagaagtc gggagacgcc
     5941 accgttgttc tggtcggctt cccgtccgtg ggaaaaagta cgctgctcaa caggctcacc
     6001 ggctctgata taagcgaaac ggcggcctat gctttcacga ccgtctccgt gatcccgggc
     6061 ttgatggaac accggggcgc gaagatccag atcctggata tccccggcct gatcgccggc
     6121 gccgccatgg gtaaaggcca cgggaaagag gtcatcgccg tcgtgcggag cgccgacctg
     6181 atccttgtcc tggtcgacgt cttcaacgag cagcatacgg acgtccttct ccgcgaactc
     6241 cacgatgccg gcatccggat caaccgaccg agacccgata tcaccatcaa gaaggccggg
     6301 tgcggcggta tcaggctgaa caccgtcggc gccgtcgacc tcgatctcga ggatatccgc
     6361 gcaatcctcg cagagagcaa gatcgtcaac gcggatgttc tgatccgcaa cgaagtcgat
     6421 caggacgact tcatcgatgc aatgatcggc aaccgtgtct acgtccccgc gttcatcgca
     6481 gtcaacaagg tcgacctggt agacgagcag accggagcgg agatagagaa tgaactgggc
     6541 gagcggttcg gtgacgcgcc catcatggtg tcggcgcaca gcggctaccg catcgaagaa
     6601 ctcaaagacg ccatattcga gaacctgggg ttcatgcggg tctacttaaa acccctcggc
     6661 ggccccgcgg atatggaaga gccgttgatc atccggagtc cggccacggt cgaggatgtc
     6721 tgcaaccgcc ttcaccggga tttctcggac aagttccggt atgcgaaggt ctggggcgac
     6781 tctgcaaaac acgacgcgca gcgtgtcggg atcacccacc aactcgcgga cggcgacgtc
     6841 ctctccgtcg tcacccgccg ctgaccgctt tttttagaac cgggagaggt gcatcggtct
     6901 ttatggctgt tcccgagtag tgtgcacggc ttgcccggta gccgagagcc gagaggttcc
     6961 cgatgacggt ctcgatgccg ccgggggaga cccgcagcga ttttgcgacg acgtggtagt
     7021 cgtagtggct cgaggtctcc agttcatggc ggcaggtatc gagcagccgc gcgatccgcg
     7081 agccggtccc ggccgtgacc gacggcagcg tctcctgcat cgcggcaagc gtcccgtcgt
     7141 cgttgatgct ccctgtccag agggggccga ccggcacgag gtccgcaccg cagtccgcgc
     7201 atttccccgg tccggggagc atcccggtct gctctgtccg gtagaggcag tgtccgcact
     7261 gcatcacgta gccgatccgc ccaagtgtcc ggtcggccgc cgccgccccg taccggacac
     7321 gcaggtgcag ccggtgaaag tgcgcatgcg cgtagcagaa gaggggttct atgccccggt
     7381 catacttgat cacctcgcgc gccacaaacc cgagcagcgt ccggaggccg acctcggggt
     7441 ggtactcggt gttccggggg tgggagaagt agcggcgcat gcctgctttt aagtgcgctc
     7501 cgcagagcgg cgcggtgtcg gtggcggtga cgaagaggta gtctccggcg ctccgtgccg
     7561 cggagtcgac gaacggtgcc ggtgtcccga aggggtcgag gtcgacggcg tcaaaccttc
     7621 tcgcgctcat cagtgcgttc gcgtcctctc cggtcacctc ggcggcaaga cccagcgcct
     7681 ccacgttctg ccggatcaat tcgaccgcct tcgcgctgcg gtcgttgatc gtcaccggta
     7741 tcccgacttc atgcgccacc cttaatcccc ggaccccgga cgccgccatg gcgtcgacat
     7801 aactcttcgg ccgcaggacg gagagcagca ggaccgtagc gtcccggttc atctccatgc
     7861 gcgagttata aaagaccgga ccgcttccag gcgggaactg gaggtgagga tcctgcttgg
     7921 gcacgaaaaa ccgggttttc ccttcagtaa cctcaacaac atccatgctt ccagaaaagg
     7981 tggatgctcg atacccttat accttctcat gagcaatact cttactcctg cgtatgggct
     8041 tgtggcctag tccggacatg gcgtcagcct cctaagctga atgtcggggg ttcgaatccc
     8101 cccaagcccg tcatttttcc aaatgccgct ccagaagacc tctggttatt tatcttccgc
     8161 ccgagaacct aactcttcga gaatgagcgc ttccgccttc atcagggtta cccgcccgca
     8221 caacgccgtc gttgccggcc ttacggcgct cctgggatac ctcatcgcca caggaaccct
     8281 tacgccgccg tcgctcctgc tcgcggtggt cgtcgcgctc atcaccgccg gcgggaacgt
     8341 gatcaacgac atctacgatg tcgagatcga ccggatcaac cggcccgaga ggcccatacc
     8401 gtcaggagag atcagccttg ccggtgcaaa ggcgtatacg gtggcgctct ttgccgtcgg
     8461 tcttctcgcc gccaccctga cgacaccgct ctgccttgcg atcgccctcg cgaacgcggt
     8521 cgtcctgatc gcatacgccg tctggctgaa gcggaccccg gttctcggca acatggccgt
     8581 cgcctacctg accgccagcg tcttcctctt cggcggagcg tttgcgggta tcgaggggct
     8641 cgtccagaac ctctcgctcg cggcgatcac gttccttgcc accatcgcac gggaggtctt
     8701 aaaagacgca gaggacgtgg acggggatgc ggcgggaggg gcacgcaccc tcccgatgat
     8761 cgtcggtatc cggcggacgg gaacgcttgc gttcgcctgt gcctgcggcg ccgtggtggc
     8821 aagcgtcctc cccttcggcg actggtgggg tcccttctac ctcgccgcca tcgcggtggt
     8881 ggatatcgtc atcctcttcg gggcctcccg gggcctccgg tgtacgacat cggcctgtgt
     8941 ccgggattcc ggagcgacgt cgatcctgcg aaccggaatg ttcgccgcgc tcgcggtctt
     9001 tgcgattgct gcggtgatat gatcgaaacg tgcctgctat gaaactcact ccggcaggac
     9061 cccggacctc tcgtggcgag cccgggttca cttttatcct ctgtgtcagg acagatatcc
     9121 ggtacggcct tccgacgttc ggaatatctc actgataatt ttatctggtc acgggccgat
     9181 ctgttatcca ggagatcttc atgaaagttt accggcacgg ggacacgtat atagcaccga
     9241 atggttcctt ttttgacgga aacgtgaaga tagatggaaa tttcatcgtc cccgcggaga
     9301 cgcacgtctg ggggaacatg gtagttgccg gctgcctcga actcgggccg ggctcaacgg
     9361 tcggcggctc catcgaggcc gcgagcatcg tcatcggtca tgacgccaga ataaaagggc
     9421 cggtccgggt ttcggagacc gcaaccatct gtgacaacgc gtgtctccat tctatccggg
     9481 caggaggcga tatcaccctt cggccgggcg tcaggatcgg ggatgtgaac agtgccgaga
     9541 cgatcttcgt ctacggcaag gtcacgagcg agcgtctatt cggcagggcc gtgaaagttt
     9601 acggcatctg acggggactc gatgccgagc agggcggcat cccttaccgc ggtccagtct
     9661 gctaccgtct ccacgcaccc gtcttttaag ttcacgacac cgatggcggt aagccctatc
     9721 cggtcggaca tatcgatccc gtctttcacc tccatgaggc agccgacgcc gatgatcgcc
     9781 cgcggatgat atctcttgac cattcgtttg atgaacgtcg agcccgggac gatgaagacg
     9841 cgatacccga gactctccag ccacgctgcg ttctcgctga ccgaacagcg gccgcaattc
     9901 cggcatttta agccttcggg ggtcaggtgt gccggacact gcgccgaccg caggcactgg
     9961 ggcaggaaga ccgcccgctg ctccacagga atctccgaga acgccttcgt gttcatggtg
    10021 tttcggagcg ttatgaagaa cgtgacgagg tctttgtcgt ccagcccgag gagcttgcag
    10081 aacgccttca cgagcccctc taagaggatc atgccgggta tcaggatccg cgggagatag
    10141 aacttcccgt tggtgatcga ggcggcagcg atgatggcga gggctactgc ggcaagcagc
    10201 atcccgagaa tgaggaagaa cgtcatctcc ccgatgatgg tcatcaactg gacccagatc
    10261 ccggagtcga agaaggtcat cgtcgcgcgc cctctgtttt gaccagctga aaagactttg
    10321 catacggcgg ccgcagcacc ccgatctcgg tgacgatcgc atcgacgagg tcgagcgggg
    10381 tggcgtcgaa ggcgtagttg agcacgttca cgctgtcggg cacgagctgc cggtcgccac
    10441 aataggcgag ttcagagcgg tcccgctctt cgatggtgat atccgactcc gtccgggcaa
    10501 ggtcgaacgt ggagatgggc gccgcaacgt agaacgggat cccgtgatgc cgggcggcaa
    10561 cggcgtgcat gtaggtcccg atcttgttaa agaccgcatc tttggtgatc cggtccgcac
    10621 cgacgatgac gaggtcgatc ttccccttcc gcatcaggta tgccgccgag gagtcgggga
    10681 tgagggtcac gtcgatcccg tcgcgagcga gttcccagca ggtgagccgg gacccctggt
    10741 tcagcgggcg ggtctcggag gcgagcaccc gcacgctctt tccggccgca accgccgacc
    10801 ggatcgtccc gagcgccgtg ccccagcacc ggcaggcgag cgctcccgcg ttgcagtggg
    10861 tgagcaccgt gcactctgtc gggaagagtt cttccccgaa cgcaccgagc ctccggcagg
    10921 tcagttcgtc ctcttgtgcg acggcggtcg cctcggcgac cgcgagcgcc ttcgcctcgg
    10981 cgaccgatgc ggccatcgcc acctttcttg ccacgcggtc gatcccccac gcgaggttca
    11041 ccgcggtcgg gcgggtgctc ctgagcaggt cggcggcacg gccgatctcc gcaagcaacc
    11101 gcccgagatc ctgctcggtg ctccggacgg ccgcgagcgc cacccccatc gcaccggcga
    11161 tgccgagcgc aggtgccccc cggatctcaa gcctcccgat cgcccgggcg aggtcatcga
    11221 cggttgcgca ccgcatctgc tcgtaccggc ccgggaggag ggtctggtcg atatagacga
    11281 tgcagccggt ctctgcctcc cacgcgatgg tgtagggctc ttccctctcc ggcatgccta
    11341 tcccaccctg accgtgtcca ggtacgcccg catcgcggcc gctcccccca tggcgaccga
    11401 gtcgggaata tccctcggcg cggtggctgt cccggtcacg tagatcccgg gcttcaccgt
    11461 gagcacgggg ccgactttct ggtcggtaac gctgaagaac ccggactcgt cccgcgggat
    11521 cccgagcatc cggctgatct cttctgctcc attggccggt tcaagcccga cggagaggac
    11581 caccaggtcc gggtggaacg tctccacctc acgggtctcc gtgttctccg cgaccatcat
    11641 caggtggtcg ttctcctcga gcacctcgcc cgggaacccg cggacgaacc tgaccccgag
    11701 gctcctcgcc cgctcgtagt actcctcgta acccttgccg tatgcccgga cgtcgttata
    11761 aaagacggtc acctcgatat cggggttctt ctcccggatg agcatggcgt tcttcatcgc
    11821 atacatgcag cagacccccg agcagtaagg gcggccgacg ctgaggtcgc gggagccgac
    11881 acactggacg aacgctatgc tccggggcgg tttgccgttc gagagccgct tgagtttgcc
    11941 gcctgtgggg ccgctcgcgt tgatcatccg ctcgaactcg aggctcgtga tcacgtcggg
    12001 gatgaggagg tagcgcagtt gcgctttatt ccgggcgtca aacgtcttat agccggtcgc
    12061 gacgacgatg cttgccgcct ctatctcgat caggcgctcc tcttcctcgc ggaggatcgc
    12121 ctccttgccg cagacgtcgt agcagaggcc gcactcgatg cagtgctcct tatccttgac
    12181 gacgatatcg gggaccgcct gggcatgggg tttatagatt gctttccgca cgccgatgcc
    12241 ggcgtcgaac cggttgtaga cctccaccgg gcagatctgg atgcagtcgc cgcacccgtt
    12301 gcactcgtcc tcgatgatat agcgggggtg cttcctgatc cggacgagga acctgccgac
    12361 ctctccctcg accgattcga cctcggagca ggtgtggatg gtgacgtttt tgtgccgggc
    12421 cacctccacc atctttggag agaggatgca catcgagcag tcgttggtgg ggaacgtctt
    12481 gtcgagctgg gccatatgcc cgccgatggt cggttcgcgc tcgatgagat ggacgtggat
    12541 gttgtggtcc gcaaggtcga gcgccgcctg gatgccggca accccggcac cgatgaccgc
    12601 gacgtcaacc atggcggtac ctccctgcga gtttgacata ttcacgcgca ttgtggacga
    12661 tgccttcctg ctgttcgggc gtcgtctccc tgaccacttt ccccggcacg ccgaggacca
    12721 gcgagtgcgc cgggatctcc ttgccttccg ttaccacggc accggcgccg atgatggagc
    12781 cttcccccac caccgcgccg ttcaggacga tggcgcccat cccgacgagc acccgatcgc
    12841 ggatggtgca gccgtgcagg atggcgccgt gaccgaccga gacctccgcc ccgatcccga
    12901 ccgggaaccc gatcgtggtg tggacgacgg cgttgtcctg gatgtttgag cccgctccta
    12961 ccgtgatcgt atccttatcc gcccggacca cggccccaaa ccagatgttc acatcgtcgg
    13021 cgagcgtcac atctccgatg acggttgcgt tctgcgcaat aaagacgcgg tcccccacga
    13081 ctgatccgct ctccatagta cgtctactgt tggttgcgtc gatacaaaat cttctccctc
    13141 ggcacaggag ggaggggcgt gtgtgtctgg tgatgcccgg gaagtgaggg actgcttgga
    13201 cgttagatgt ggtgggatcc ggtgcagtga accatggggg actgcgcacc ttcggtgctc
    13261 gaactcc
//
LOCUS       DAJB01000037           25029 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_78233, whole genome shotgun sequence.
ACCESSION   DAJB01000037 DAJB01000000
VERSION     DAJB01000037.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 25029)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25029)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25029
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_78233"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         4903 a         7782 c         7518 g         4824 t
ORIGIN      
        1 gtctcgttgg cggtcccgct ggtagtctcg tccagggttt cctcgggggt ctcttcatcc
       61 agggcctgta ctacggtctc gttatcaagg gtctccgttg gggtctcgtc cagcgtttca
      121 tcgtccaggg tctctaccgg agtctcgtcc agagtctcct cgggggtctc cgtcggggtc
      181 tctacccggg tctcgtcggg tggtgtcgga gggagagtcc aggtcaaaga agtcccgggt
      241 gctgtgctct gggctgccac ggggggcaca acagccgctg ctgccacaag aagcacaacc
      301 aggagtgctc tccatgtcca ttggtttcct gccatgagaa ctcccgtttg gcataatcct
      361 atttttatat ttcggtcgtt ttcaaaatcg tatcgacagc taactgtccg atgcgcccca
      421 caacatggcg acacctcact gttgcacagc agtgaaccgt caaaaaacga gatgccggta
      481 cagtgatccg gacgtgaaat gtgggcagct aaagaccctt ttatcgtggc tggtcccgga
      541 ggcgaatccc tgtctgcggc ctttcaacag agaccgacct gccgtactgt cagcgggaaa
      601 gaagtagcga tcagattggt gcttcaccaa tcttccggat caggttgtcg accaccacat
      661 cgcgcattcc ctccacgaac ttgatgctcc cgacgacaag gtggccaccg ccgctcactc
      721 ctccgccgga gatctcgcta tggagttcgc gcaccattcg cggaatgttc atcatgacgc
      781 cacgcgaacg caggacggca aagtccggcc cgaacccgat cgtgacgacc ggttctcccg
      841 ggtgcgcccg gaccagccgg tcatgcacct ctcccgaagt cttgccgggc ggcgggaacg
      901 tgaaccggtg ggcaaagagt tccacgtcga gcatgaagag gtgggcgccg ttttcgagca
      961 tccggctctt gacgtggggc atgatggcct ccagctgctc ctcgatcgcg tgattcgctt
     1021 catcgaccag caggttgacg aactctttat ggcgttccgt gtcgccggcg aggttcagga
     1081 tagtcttgac gatctctctc ccgtcgctga accggagcca gaactgctcg tagtcgagcg
     1141 ccagcgcgat cgcgcggcaa ttgtcctccg aatactcagg cgcggcaacg gcgaggtagc
     1201 gggcacgctc cggcgcttcg ctccggtcgc cgagcgccgc gaccgccggc aggtgccgta
     1261 tctgattctc gactgccgga ttgaccatgc gggcgacctc tgtgcccagc atcccggcgg
     1321 tgatcccgta atcgccgccc acatgatagg ggttgacgtg cccgatcagg taatcgtcca
     1381 cgatctcgtc ggggtggtgg tggtccacga ccatcaccgg gagaccgaag atccgggtca
     1441 ccttcagcga cggcatgtct tcctcggtgg acccgttgtc catcaggagg atcatcggca
     1501 tcttctgccc gtagcggacg ttgtccttga gggcgaagtc gagatcccgc gtgatatcct
     1561 cgatctcata gaacggagcc ttggacgggg accgcttgaa gaggaagaac tccgcatcga
     1621 agtccccgcc gctctcccga agcagtgcgg tcaccgcctg ctcgatggca acggcagcgc
     1681 atatgccgtc ggcgtccgcg tggtgccgga gaatgatcgg ccgggccgac aggaccgctt
     1741 tccggatctc ctttgctacc tggcgcatct gcggcctcaa ggcttccaga acctcactct
     1801 ccacgaggaa cgggatgtcg gacggttccg cacgctcatc gagcgccgca tcgatacgcc
     1861 ccctgacccg tgccgcatcc tccggctcca ggacggccat ggacgcgacc tcgatctgga
     1921 gctggttgtt gcgctgcatc acctcaccca tgagggcgac gatatccccg agttcgacct
     1981 cgggataggc ccggacgccc gcctcgatga acgcggcccc gttcgcggtg ccggactcgt
     2041 ccacgatggt gaagatcgtc ggtcccgacg tctgcttgac ctggacgatc tcgccctcga
     2101 tcagcaccgt tctgccgatc ttcttgccga tatcggcaag aagcacgctc gtcgtcttct
     2161 tggtcacgtt ctcggtctgg tagacggtcg gcgtgacctc ctcgaggtcg atgttgccgt
     2221 tgctccggat ctggaggacg tgaacgatga tcggatcgcg ttcctcgtgc tgcaccctca
     2281 cgttgctctt atgaacgagc cccttgaccc ggtcgtttag ctggacgaat acgccgaacg
     2341 gagcaaaacc ctgcacccgt cccaggtaac tctttccttc ctccacctcg tcgatatcgc
     2401 agtttgcacc gaggcggtaa acaattgtat cttccgtatc tcccatagta gttcacctaa
     2461 ttcattcttc gggcatctcg acggcgtagc cggcaagttc atagagccgg cactcaccgt
     2521 cccgtctgcc cttggcgata ataatatccc cactctccag tcggacactc ttccccgggc
     2581 ggtagaccca ccggtcgccg tgcttgatgg cgagcacgac catcccggtc accgtcccga
     2641 gcgacgcctc cttgagggtc ctcgccacga gcggcgagga cgccgccacg ggtatccgcg
     2701 tgatgatctc gtccgactcg cggacgatct ttttgaagac cggcgggatc tcgatgtcac
     2761 gcaggaggac gtcgacgatg gagtgggccg aatcgctgat cgcctgcgca aacgaggaca
     2821 tatacaggag gccccggagg tagcgcacat cgtcgatctt tcgtgctgct tcaaggatcc
     2881 agaggtccag gtcgtagcgc atatcatcga gcgtcgaatc aagggcgacc acctcgtgcg
     2941 ccacttcctt gttgttgaag agcagggagg tgtacgccag cccgacagag agctccgaga
     3001 gattcttcat ctcgatgatc agctggaccg cccggtcaag gtcgtcgacc tcacccgcat
     3061 gcgagggttc gcccccgggg cgctccatcg cccccgccat caggaagagg atatcctctc
     3121 cggcttccgg gccccgcaca atcaggatat ccccgctctc cacccgggtg gtcctgtcgg
     3181 gatcatagat ccagcccttt ccacgccgga tggcaatcac ctgcatgccc gtcgtgctct
     3241 ggactttgag gtcgccgagc gtgtggccgg caagatcgct tcccgggctg acgatgaccc
     3301 gggcgacgat ctcttcggcc tcagggaaga cgcgcttgag ttttgccgga aacctgatgt
     3361 cttttaagat gagttttgct atgtccgaag cagagttcgc aatcctctcc gccgcctcgg
     3421 caacctggag catgccgctc atcgcttctg cttcttcgag gcgcctggca ccgaggacac
     3481 tctgaattct ggcctggtag accagtttgt tcatgctctc ctcgagattg atcacttcga
     3541 gcgctatctc tttactgtcg aagaggatcg cagagtaaga gagatcaacc atcagctcgg
     3601 agacatcctt catctcgatc agagcgtctt tgaaactgat cggctggtat tcacgttccg
     3661 tcatgggtct gttaaccgtt gttttcatat acttttcact tacaacagca ggcctatcac
     3721 tgccacgagc gtagctgcac caatcaggtc gatcaggctc gtgatgacag ggatcccaaa
     3781 gttatcggga tcgagaccat accggaatga aaggctggca gtgacgtacg ccacgccgtt
     3841 caccagtgtc atgacgacca gacccgctac aaggctgata atgaccagca ttccaaggcc
     3901 ggggctgttt agccccatga gcaccgccgc accatgcgcg atgagcgcca gcagtggaag
     3961 cagtatcaaa gtatagagat aggaaataat aaaatgatgc acgacgccgc gctcggggag
     4021 gaacgagggg ttgagctcgc cggtatgcat ccccgtcgat aggcgcgacc cgagaatgcc
     4081 gcctatcgac ccgagaccac ccataaacgg gggtatcagg acgagaaaga ccgcgagcgc
     4141 catcagtctg tcgagattga gcgagtaggt cagaccggcc agcgtgccga ggacgctgag
     4201 cgggatgagg aggtagaggt tctcgcggac gattgggccg atcgccctgg gccgctgccg
     4261 ggtgtagagg atagtgacgg cggcaacggc gaccaccacc acgccgagca ccaggcgtgc
     4321 agagggtggg agggacacga cgagtgccgc ggagaggatg aggatcggaa gcgtgacgat
     4381 atccccggag gtggtgacgg tcggggcagc gatcatgtcg aggtcgaggc cgtagcggta
     4441 gcttacgagc acgatgacca gggtgatccc cattacgacc agccccgata cgatgccact
     4501 gacgaccgag atcagcatga gatcggtgac gcccaccacg ggaaggccga aaaggaggct
     4561 caccgcgtag gcaaagatgc cgaggatgaa cgctatgaga atggttatca cgaacgatgc
     4621 gcggatgttg tcgccgagga cagagccctc atggaaatcg atggagaact ctccaaggtg
     4681 catcgacgac gacaggcggg acgcaaagac cccggcgatg ttcccccgca tatcgatgat
     4741 cgacggcagg agcaccagca ggccggggat cagcgcgagg gcctcacggg cggagccgag
     4801 gtagataccg gcaatgctcg aggcaacggc actgacgagc agggcgccaa gaccggtcag
     4861 tatcaggcgg ctctggctgg ataggccctg ctccatcccc atcaccgtcc atctcaatca
     4921 ctccggtacg ggatctccag aacggccaga tcggccggca ggatcacgtc tccctcatat
     4981 tgtcgctttg catcgcgtat atggtttact gtgctggtat aacgggaact tatgtgcatc
     5041 agggcaagca tacgggcgtt caaggcgacc gccgcctccc cggcctcccc ggcggtggag
     5101 tgcaaaacct cccgggcgcg gtcgctctcc tggtcgtcga acgtggcgtc gtggatcagg
     5161 atatctgcgt cccggagcat cgcggtgatc ccggggtggt gctggagcgg ccgtgtgtcc
     5221 ccggtataaa ctaccttccg ccccggacgc ggctctccca gcacatcggc gggccggatc
     5281 tcggtctcga cgccgtcgcg gacgatgcgg accgcttccc cgcgctgcag ccgtccgaag
     5341 agcgggcccg gcgggacacc gagcgcaacc gcctgctccc ggttgaacct tccgggccgc
     5401 tcgtcctcct ccattacata gccgagcccc ggaatgccgt ggtgcgtaga aaacgcccgg
     5461 acggtgtagc cgttgaaggg cacgaccgaa ccgtgctcga gggagtgagc agtgacggag
     5521 aagccccggg tgtggcggct taccttctgc acgaggtcga cgaactcgcc tacccaggac
     5581 gggccgtaga tcggaagcgg gtcggtccgt cccatgaacg cgagcgtctc gacgagcccg
     5641 aagacgccga ggaaatggtc ggcatgccag tgggtgatga agacggcgtt cacggtaaac
     5701 ccggtccggg cacgcatcat ctgctgctgg gtgccctccc cgcagtcgaa gagcagggtg
     5761 tcggagcccc gccggatcag gatgcaggag gggttcctct gcggtgaggg gagcgctccc
     5821 gccgtgccga ggaaatgaag gtgcagggtc tcgccggcta tacgaaccac ttcctgaatg
     5881 ctgcaaggct ccgctttgcc tcttcgagtg tccggccttc gataacaacc gggccggagt
     5941 agtcgcgggc gacgacctgc ccgaccttct cccaggcgat ggtcccatcg ccgagtgcca
     6001 ggtgctcgtc cgacttcccg tggttgtcgt ggatgtgcag gtggctgatc tcccggagat
     6061 gggcaaggaa cgcatcgacc tggccgttgg tgttggcgtg ccccacatcg agcgtgaccc
     6121 cgatccccgg gataccttcc gtgaggccga gtatctcctc ggggtagcgg cagaggaagt
     6181 ccttgatgct gatcatgttc tcgacgcacg caaggatgcc gtgctcctcc gcagacttcc
     6241 cgatctcgac gagtgctgtc ttctgcaact cccagacctt ctcggggacg agttttccca
     6301 cgggagatac aaacccgggg tggaacgtca cccggtcggt caggtcggct gcgtggtgga
     6361 tgcagcagca ggtctggcgg atggactcgc gccagatggg gtagtttaac gatgcgaggt
     6421 ttaagtcgct gtagggagcg tgaacggtcg cgaaaaggcc ggtgctctcg aggttctccc
     6481 ggatagcggc aaaattctcg gggttgtcca ggcggtactt cccgtcggcg acgatctccc
     6541 agccggtgta cccgagttcc tcgagcccga agacccagtc ccgggattcc cagacttttg
     6601 atgaggatga aaaatacgga gcaagactca ttctattcct cccggccggc agagcagggc
     6661 acggatctct gcggcaaact catcgagtgt tccctcgttc gtgatccggc agtccgcgag
     6721 atcgagagcc cgaccgagcc cccacccgag ttcccgctca tcgcggaccc ggagctcttc
     6781 ggcggtgagg gagtcgtcgg accggccccg gttcgtgagc cgccggtaac gcgtctcaaa
     6841 cgacgagacg atcccgatga gggtgaagtc ggggaagtgc tccttgaacg ccgccacctc
     6901 atagtccccc ctgatgccgt cgaccaggac caccggagcg gactctgcct cgatggcagg
     6961 gatggttatc cgggcgatgg cgtccatgcc gaggtccgcg cgcaacttcc ccgacattgc
     7021 gccgaggttc gcgtccgtcg gcagaaggcc ggcctcctta acccgttctc gtatcgcatc
     7081 ccccatgacc acgaccggaa tcccgaggtc ccgggcgatc ttcgacacct ctcctttccc
     7141 gcttgccggc ataccaacga ttccaatgac tttcatcagg gctccttcat ctttgttatg
     7201 ctatcgtatc cagaacagcg ggccgcaccg gtgagagacc ggctgcaggg tcccggcggt
     7261 gcaggttgcg gcggccgcca caactatctc cataaaagtg ggttgcagac gatattatgc
     7321 ttaacgtcac cggaacggtt actgctccgg gccgtacccg acctcgccgt cctcccgggt
     7381 ccgtatccgg actttccggc cgagcggcgc cgctaccgcc tcgagttcct gccgggtcag
     7441 ggggacgaag cccggggcca ccccctgctg gagcacgagg tcgaggtcac gggtagcctc
     7501 tgcaatcgac gggagatcat cctcgcggcc ccggaagagg gtgaccacag cctggcaggt
     7561 cccaaggctg ccgtcggcct tcgcccgctt gcagacggcg agcgactgct tcaccgcatc
     7621 ggcggcagca agttccagga gttcgtcgta gcgttcccac gttgtcttga tatcgagcgc
     7681 gatcatctcc accaggtgcc ggccggtcag gtcttcgagc acgcggggaa agatgccgtt
     7741 cgtgtgcagg cccacagaga gccccatgtt ccgggcggcg gcggcgaggt ggacgagcgc
     7801 cggcccctgc agggtcggct ccccgccgga gaagactacg gcgcctgcaa ccgtgcggga
     7861 actctggatc atggcgatga tctcgtctgc gtcgcgaagg tcttcgccac cctggatggc
     7921 gatgttatgg cagtagaagc agcgcgcggg gcaccccctg aggaagacgg tgcaggccgc
     7981 ccgcccccgc cagtcgacgg tgctgatcgg gacaaaacct ccgaaattaa cgtgcaaaca
     8041 atcaccagac gggatagagg tgctccctga tttcatgtaa gccttggctt tccggctatg
     8101 aaataagatc tgcgtaagag taagtcaagt ttacttgtcc agagaacaac ccggttttag
     8161 ccaggatcct aaaaacaagt ctttatcaac tcttttcctc aaatataagg ccatgggtct
     8221 catagaagac gcccgacgtg gcgtcgtcac cgaggagatg cggatcgtcg cagcagcgga
     8281 aggggtctct gaggagtttg tcaggcacgg cgtggctgaa ggccacatcg tcattccggt
     8341 ctctccctat cggaaggtga agatctgcgg tatcggcgaa gggctccgca cgaaggtcaa
     8401 cgcaagcatc gggacgtcga cggatatggt cgacgtcgat gtggagatcg agaaggtccg
     8461 gcaggcagag cgtgccggtg ccgatacgct gatggagctc tccaccggcg gcgactttgc
     8521 ggagatccgg cgccgggtcg tcgaggcgac caccctctcg gtcgggtcgg tcccgctcta
     8581 ccaggcgttc atcgaggccg cccggaagca cggggcggtc gtccacatgg aagaggacga
     8641 cctcttccgg atcacggcgg agcaggcgaa gctcgggacc aacttcatgg cgatccatac
     8701 cgggatcaac tatgagacga tgaagcgcct gaagaaccag gggcggcacg gcgggctcgt
     8761 ctcccggggc ggggccttca tgaccgcgtg gatgctccac aacgagaagg aaaacccgct
     8821 ctatgcggag ttcgactact tagtcgagat cttgaaggag cacgaggtca ccctctcctt
     8881 cggcaacggg atgcgggcag gtgcggtcca cgacgcgacc gaccgtgccc agatccagga
     8941 actgctcatc aacgcggaac tcgccgataa agcgcatgaa cagggtgtcc aggcgatcat
     9001 cgagggaccg gggcacatcc cggtcgacga gatccagacc aacgtcgtgc tgcaaaagcg
     9061 ggtgacgaac cggaagccgt tctacatgct cggcccgctc gtcaccgata tcgcgcccgg
     9121 ctacgacgac cgggtggccg cgatcggggc cgcactcgcc tcctcctacg gtgccgactt
     9181 catctgctac gtgacgccgg cggagcacct ggcgctcccc acccccgagg aggtctacga
     9241 gggcgtgatg agttcgagga tcgccgccca cgtcggggat atgatcaaac tcaagaagcg
     9301 ggatgccgac ctcgagatgg gacacgcccg ccgggacctc gactgggagc ggcagtttgc
     9361 ggtcgcgatg aaccccgagc gcgcccgggc aatccgggcc gagcggatgc cggccgatac
     9421 cgacggctgc acgatgtgcg gcgattactg tgcgataaag atcgtcggac ggcattttaa
     9481 cttctgatgg ggatggggga gggcgcttcc cccgggatct cgtctttttt atgggtattg
     9541 tttttcgggt agcgggggtg ttcgttcttc cgggtggtag actgcatgag ctgcagagcc
     9601 agtcttatcg tgcttccttg ctctttgcac atatctgaac ggccactatg ggcaagtccc
     9661 agtacagtgg accgtgggga tatccccacg gtccagcgta cccggagcgt gggaaaatcg
     9721 gtctaatacg cttgcacaca agaacacccg aacatcctca catcaaaatc cacaccatta
     9781 tcgcctccct cacccacgat cacgtaagag gacagcccaa tgaaacccga taacgctcaa
     9841 ctcgtgaacg aattccttgc ccacgccgac gatctcggcg acgacgtcat ggaagatgta
     9901 aaggagatgc tcggcgtcgt gccgttcatc ttctccatcc tccgggaccg gcccgacatc
     9961 tttgcgctct ccgccatcgc cgactaccgg atctcccggc caggctccct cgacgcaagg
    10021 accgcggaac tggtcgccgt tgcggccgcc gcaggggccg gggcggacag ctgcctgaag
    10081 gtacacatgg gggccgcgct gaaggaaggc gcatcccgcg acgagatcct cgacgccctc
    10141 ctgatcgccg ccatgatcgg caagacccgg gtccttgcat cctccctccg ccagttccgg
    10201 gaggtctgcc gcgaggagca gacagcagag aagtagactg cttgccggcc cggagcatgc
    10261 atgctttatg gtctgattga gccaaacctc tcttgcaatg agccagacaa aggcggccct
    10321 tcgccagcag gcaaaggagg cccgatccct tctctcccct gcagagattg ccacatacag
    10381 cagcatcatc gaacgaagac tcctcgagat cctgaacgga tttaagaccg tcatggtcta
    10441 cgcctcaaaa gccccggagg ccgagaccaa cggcctgatc gcggacttaa accgccgggg
    10501 cgtgcgggtc gtcgtgccca tcatcgaacg ggagacctgc agcctccgcc tctcctacct
    10561 gccggacgcc gcggtccttt tccccagcac attcaacgtg cccgaaccaa tcggccacga
    10621 gctcccggcc cggcccgagg agctccaggc ggttgtcata ccgatgctcg ccttcgacgc
    10681 cgaaggaaac cggctcggct acggtgccgg gtactacgat cgcttcctct gccggcaccc
    10741 ccacccacag aagatcggca tcgcgttctc ctgccagcag gcagagagca ttcctgccga
    10801 cgataacgat gtgaagatgg attacatcgt tacagaaaag gggatcatcc agcacaacgg
    10861 gagggagagt taaggaaaga tataaatact gtcttgcact tacctatggt aaaccctaca
    10921 ggaggagatt gttcacatgg ctaacaccga atctgttgag gtgaccatca aggaggcggc
    10981 tcacgaagac gccggccggg ggatcgccag actgagcatc gataccatga aggctctcgg
    11041 tctcgtcagc ggcgacgttg tcgagatcga agggcgccag aaggcggcga cgctcatatg
    11101 gcccggattc ccgcaggaca ccggcaaggc gatcctgcgc atcgacggca acacccgggg
    11161 caacgtagga gccgggatcg atgacaacgt ccggatcagg aagaccgagg ccggatatgc
    11221 caagaaggtg accatccagc cgacccagcc catccgcctg gtgggcggcg agcagtacct
    11281 gggaaggatc cttcgcggcc ggccggtcac agaagggcag cacgttcggg tcagcatcct
    11341 cggcaacccg ctcacgttcg tcattgccag agtgaccccg aaaggtatcg ccatcgtcac
    11401 cgacagcacc gagatcgagt tgaaagagac tccgtatgaa cccgaggaag ggcggcgcga
    11461 aactgcgacc gacgtccact acgaggacat cggcggtctg gaccgcgagc tgcaactcgt
    11521 ccgggagatg atcgaactcc cgctccggca ccccgaactc ttcgaacgcc tcggtgtgga
    11581 gcccccaaaa ggcgtcctgc tctacggccc gcccggaacg gggaagacgc tgatcgcaaa
    11641 agcggtagca aacgaggtgg acgcccactt catcacgctc tcgggccccg agatcatgag
    11701 caagtactac ggcgagtccg aagaacgcct gcgggaggtc tttgaggaag cgcaggagaa
    11761 cgcaccctcg atcgtcttca tcgacgagat cgactcgatc gcacccaagc gcgaagaggt
    11821 gaagggggag gtcgagcgcc ggatcgtcgc ccagctcctg gccctgatgg acggcctgaa
    11881 gacccggggt caggtggtgg tgatcgccgc aacgaacctt cccgatatga tcgatcccgc
    11941 cctccggcgc ggcggccggt tcgaccgtga gatcgagatc ggcatcccgg acaccaaggg
    12001 aaggcagcag atcttccaga tccacacgcg gggcatgccg cttgccgaag atgtgatcct
    12061 cgacgactac gcccgctcca cgcacggctt cgtcggcgcc gacattgcgc tgctcgcaaa
    12121 agaagcggcg atgcacgcaa tccgccggat cataccgcag atcaagatcg aggaggagat
    12181 ccccaccgag atcatcgacc agctccgcgt gacgaacgaa gacttcatcg aagcgcataa
    12241 gcacgttgag ccgagcgcga tgcgagaggt gctcgtagag atacccgatg tcaaatggga
    12301 ggacgtcgga ggcctcgaag acgtaaaggc cgaactcgcg gaggccgttg agtggccgct
    12361 caaatatccg gagatatttg cctccttaga caccgaaccc ccccgcggca tcctgctctt
    12421 cggacccccg gggacgggca agacactgct tgcaaaggcg gtcgcaaacg agagcgaaag
    12481 caatttcatc tccgtaaagg gtcctgaact cctctcgaaa tgggtcgggg agtcggaacg
    12541 cggcgttcgt caggtatttc ggaaagcaag gcaggcagcg ccgtcgatta tctttttcga
    12601 cgagattgac gcgcttatgc cgaaacgcgg atcttatata ggatcgtcgc acgtaactga
    12661 aagtgtggta agtcagatcc tgacagagct cgacggcctc gaagagctca acaatgtcgt
    12721 ggttctcggc gctaccaacc gcccggacat gttggacgag gcgctgcttc gccccggcag
    12781 attcgaccgg atcatctacg tcccgccgcc tgaccgggaa ggcaggaaga agatcttcga
    12841 agtgtacctc aagaacagag agatccttgc aaacgacgtg aacatcgagg aactggtcga
    12901 caggaccgag ggttacgtcg gagccgatat cgaggcactg gtccgtgaag ccaagatatc
    12961 cgccatgcgc gagttcatcg ttgcgatggg gggtaagtcc gaggaagaac ggcgccaggc
    13021 gatcggcaac gtaagggtca cgaaaaagca cttcgaggat gccctctccc gcgtgagagg
    13081 cacgctggat atcgaccggt tagaagagaa cgaacgccac tcctggcaga tcctctacaa
    13141 ccaggagcag cggtctgcgc tcgaagacgc catctcgacg atcaaccgtg ccaggatgcg
    13201 ggagactgag aaggtcgagc aggaagtcaa ggatctgacg gaggccctga aagatgcggt
    13261 ctaccagagg aagaaagact tcggcgagat caagcgcctc accaaggagt tgaagaccag
    13321 gatagaacgt cccctgccgc agacggctat ggcgttctga cgggagtgcc ccgtggatgg
    13381 ggcgctcctc agggaggtca tgcgacctcc tcatcaacag agacagaaac aacgtgatgc
    13441 acatgagtga cagcccagca gacatcttcc agcaactcaa cgaactgatg aaacgcctca
    13501 tggagcaggg gctcaaggag cagggagatc agaacagacc gtttgcctac ggatttaaga
    13561 tcgtcctcca tgacgccggc aagaccgaga ttccggcgga ggaagcggtt cccgcagcag
    13621 agccggagga accctcatcc ggggggacca tcgagccgat agcagagatg tacgcaaccg
    13681 atgacgacgt gacggtggca attgatctcc ctggtgccga tacggagaat attcacctgt
    13741 cactcatcaa cggcacgctg acgatcatcg caggcggcaa ccagctgacc tcggtggagg
    13801 tgccggccgt ggacgccgac tccatgcagt cgaactacaa gcacggcgtc ctggaggtca
    13861 aattctcccg gggcaaatcg atcaggatcg actgagacgt caaagcgcct gccggaccag
    13921 acccggcaca atacatttta tcgacagctg caccccgtga gcagaccgga ggccacaggc
    13981 cggcaggtgc cgcgcgggga ggctgcaaca tgtccctccg ctccgtactc ttctcatgtt
    14041 agccctcctc ccggccgcac ccggacacta cttcccacag aggacgcgcg atcgtcaccc
    14101 gggtctccac acgagaggtc caaaaatgct gccctcgaag atgctccaat tcgcagcagg
    14161 tccggagaaa acggcaaaaa gagtaaacgg cggtatactg gccctggtgg tgccgtagag
    14221 gagatatgac gggaaggatc gtccttctca gccatccaga aaaatggttg ccgtagcaaa
    14281 ccatttttca aggcaacaac acctacatat acagtaatac ctatctacta gtcgtaaggg
    14341 ctacgcccct ccaggtagtg gtgatgaaat ggttaagacc accgtgtcca taattaaagc
    14401 agatgtaggc agttttccgg ggcattcccg cacccacccg agactccttg aaaaggccgc
    14461 acagatgctc aaggaagcag aaggcagcat cataaccgac tcgtacgtca cccactgcgg
    14521 tgacgatctc gagttgatca tgacgcacac caagggcgag gacaatgaag agatccacaa
    14581 gctggcctgg gatatcttcc tggaatgcgc cgggatcgcc aaggagatga aactctacgg
    14641 cgccggtcag gacctgctcg cagatgcgtt cagcggcaac gtcaagggga tgggaccggg
    14701 tgttgccgag atggagtttg aagagcgcgg ttccgacccg gtcctggtct tcatggccga
    14761 taagaccgag cccggcgcgt ggaactactt cctctatcgg atcttcgccg accccttcag
    14821 cacatccggc ctcgtcatcg acccgtcgat gcatgatggg ttcaccttcg aggtccacga
    14881 tgtcatagag aagcggaaga tcctcttcaa cacgccggaa gagtcctaca gcctccttgc
    14941 ctatatcgga gcgccgagcc ggtatgtgat caagtacatc tggaagaaga acggcgaaat
    15001 cgcggcatcc accagcaccc agcggctgaa tctgatggcc ggccggtacg tcggcaagga
    15061 cgacccggtc atgctcatca gggcgcagag cgggttccca tcggtcggcg aagtcatcga
    15121 ccccttcagg acacccatcc tcgttgccgg ctggatgcgc ggttcgcacc acgggccgtt
    15181 catgccggtg ggcgtctgcg acgcaaactg caccgcgttc gacggaccgc cccgggtcat
    15241 ctgtctcggg ttccagatca gcaatgggaa actgatcggg cctgcagaca tgttcgacga
    15301 cccggcgttc aaccttgtcc gcacccggtg ctgcgagctc tccgacgtgc tccgggccca
    15361 cgggccgttt gaaccgcacc gcctctcgct cgaggagatg gagtatacca ccctcccggg
    15421 cgtcgagaaa cagttcaagg accgctggga agacctttcc gaataacccc cttttttccg
    15481 gattgaccgg ccgcagaact gttggttctg ccggcggttg tttatggtgc cttattggcg
    15541 gccgatgaca cctgatggtt ggcctactat cgccgctcgc cggtggcacc tggcggtgcc
    15601 acacctccca ccggccgctg gcggccggtg gtcggttata ggaaaaactt aatgcgtgaa
    15661 gataaccata taggaacaga tggttaacgg catcgtactt cctgtaaaga aggttttttc
    15721 actcgtggat tctaaaatcg tcgttgagat caaggatgac ggcaggaaac tccagggacg
    15781 actcgtggca gtggatgaac acctgaatct ccacatggac gagaccaccg agtataccgg
    15841 tgatcagagg ggccgcacac tgggaactgt tgtcatacgc ggcaataata tcctgaccat
    15901 tgcacctctg ctctgatagc catggccgac caggaggaag aagcactcaa agttatccag
    15961 tcccaccgtc agggcgttct ccagagcgat ctctggaaac tccttggtat tgacagcaga
    16021 aagtgctcaa ggattgtaaa gcggctgctg gatgccgggt tgatagaacg catcgagttc
    16081 cgcagcgacg gcatcaagac ctatctcctg cgggcgaaga agcgggcgat cgaccccagc
    16141 gtcatccttg caggaggaga agttctcccg tgcatcggct gcgatcggga atgcacccca
    16201 gaagagtgcg cgctcctcct cgactggatg taccagcttg ctcttgaaga gtatcaggag
    16261 tagggcgcct cttttttgag agccagatgg ccttcagctg aggcagcgga agtcattctg
    16321 aaacgccggt tccggagtgt cgctacaaaa gagtggaggc cgggatccgc ggaccccgtt
    16381 caggaccggg caatctcgcc cggcgcgctc ttcttggggc agaccgcatc gatcacgccg
    16441 tgctgcacgt tgtagaaact gtgcgggacg tcaatctcgc attcgagatc gatgaccgtt
    16501 ttttcgtcac ccccggtgca gagggtcacg gaatcttcct ggaacgtgac gtacggggcg
    16561 ccatccgccc gggcatccgt caccgcggtg atgtagatgc agtcgtcccc ctcgaccact
    16621 tcgacattgg actcatcatc ctcgtccccg gacgggtcga gatagggtgt cccctcggac
    16681 gacccgctga tgatggtgaa cccgatgatc ctggggtcct gggggggcat ctcgctcagt
    16741 atcttctcca tcagccgcgc gatattttta aacatttcgt cgtatggatt attcgccatg
    16801 tgtaccacgt atccggtatc ggtcgttgat tcgctcgaca acccccatgc gtatgaggtt
    16861 gcccagtctc tgtttcagct catccgtgga caggctgctc acttcgtcga gttcaacgag
    16921 catctggtcg gcgtgggaga gggcgaggat gatctccata tccccttcat ccgtgacgaa
    16981 gccccccctc atgcgcacga cctcggtgaa cctcgattcg atctcccgct ccagttgttc
    17041 gaggttatcc agaagttcgt cgcgagccct gaccatcctc ctgaatgcct cgagattcct
    17101 ggtaaatcgc gttctctggt tctcttcaac ggagggaagc gtaaccccgt cctgcttttg
    17161 caggtttacg atgacattga tgtcatgcga gaggtagtaa tactttcgcc tgcgctcgtc
    17221 ctggcaggag atgaggatct cttcgcgttc catcaactgc aggtgttcta taaccgcttt
    17281 tggactgagc accaggcgct cggagatctc ggtcacaaaa cacggcttct gcctcagcag
    17341 ttctattatt cgccgtctgt tccggtttcc caggatatca aggagacggg aaacctcact
    17401 gccctcgagc atgtttacta aatgttagtg attcaggtat aaaaaaaggt tactcgaaga
    17461 ggcggtaatt cggcgcctcg tcggtgatca taatattgtg ggggtggctc tcgccgtagc
    17521 ccgcaggcgt aatcctgagg aatttgccgt tcttctgcag atccggtatc gtctttgacc
    17581 ccgtgtaacc catcccggac ttcaggccgc cgatgagctg gtagataacg tccgagacat
    17641 ggccaacata gggggtgact ccctcaacgc cctcgggaac gaacttggtc ctcccgaact
    17701 ccttcttctg gaagtagcgg tcgctcgact cgccgctgct catgacgccg agcgatccca
    17761 tcccgcggta ctgcttgtag cgccggccct tgatcgtcgt aacccttccc ggcgcttcat
    17821 cggtgccggc aaagaggctg cccgccatga tacagtccgc acctgcggcg atcgccttcg
    17881 cgatatcccc ggaataccgg atgccgccgt cggcaatcac cgggacgccg accccgtgcg
    17941 ccacctcggc gacgtttgct attgcagaga cctgcggcac gcccacgccc gcgacgatcc
    18001 gcgtcgtgca gatggaaccc ggcccgatgc ccaccttcaa cccgtcgacg gagtcgacca
    18061 gagtagaggc cgcctgcttc gtggctatgt tcccggccac cacgtcgacg gcgacgctcg
    18121 ccttgatctc cctgaccgct ttgacgacgt tcatgttatg accgtgagcg ctgtccacga
    18181 ccagggcgtc gacccccgac tcaaccagca gcatagcccg gtcgaagtcg aaggggccca
    18241 ccgctgcggc gacccggagt ttcccgttcg catcgcggtt cgcgcgggga tactgccgct
    18301 tttcgaggat atcgcgcatt gtgatgatcc cgacaaggca tcctcttgcg tccacaactg
    18361 gaaggcgctc gactttgttc gcatacatgg tctcgagcgc gttctccgcc gtgacatctt
    18421 cagacgccgt gatcaacttc ttggtcatgt aaccggtgat cttcgcctcg ccctgtttgg
    18481 gcaggatcgc cctgatatcc ctgcggctga cgatgccgat caccctgtcg ccctcgataa
    18541 caggcacgcc gccgatacca tgctgcctca tgacccgttc cacatcggtg accgtggact
    18601 ccgggccgac cgccacgacc tcgcgctcga tcaggtcctc agcctgtttg acgatcctga
    18661 cctcagcgac ctcgtgctcc ggagtcatgt tccggtggat aacgccgatg ccgccttccc
    18721 gggctatggt tatcgccatc cccgattcag tcaccgtatc catggcggca ctcacgagcg
    18781 ggatgttcag ggggatgctc cgcgagaacc gcgacccgac gtcgacctca ttgggctcga
    18841 cccacgattc ggagggctca agcagcacgt cgtcaaacgt aaacgttgtt tccaggttca
    18901 tcttctcgat atacatacat cacctgagca tcatcattgc tttctccacc agttcgaccc
    18961 ggatcgtggt ggaccgatcg cccccgcaga ccattccccg gacgccgatg atctccgggt
    19021 cgatccgctt taaggcatcg aggtcctcga acttcagcga accggcaagg gccgtctgca
    19081 acccgagttc ccggttctgt tcggtgaacc gggtcagtgt ctcctcgtcc atgaactcaa
    19141 agagactctt cccatcttta atgccggtgt cgatcatggc gacatcagct ccggcatccg
    19201 caaccagtgg accgatggcg aacggcgaga tcgtacccat cctctcaaaa tcggcataag
    19261 cggcgataac gacgtacttc tcaggaaact cccgtttcac cgccgcggtc accgcttcga
    19321 tgacgtcacg ggcacgatcg gctccgtcga acatcaggcc aaccttgata aaatcagcac
    19381 ctgcatgtgc ggcaccgtag gcagaaagag ccgcagttcc tggcttatac tcattgtccc
    19441 cgattgcagc gctgacaggc ttgttgccgg cgagggtttt tatctcccgg atgatccagg
    19501 gaaaattcgc acccaacgag ccttctgagg gttttttaac gtcgatgatg tcagcggaaa
    19561 gcgagcttct tgcctcctca atgctgcttg gactgacgag taattgcata gaaattaatg
    19621 atccttcacc ctaatagtga ttgcgttgac gacatggaac tgattcttgc agttgatctg
    19681 gcagacggtc tggttgtgca cgggaagtcc ggcgaccgcg ccagttaccg gccgctaacc
    19741 tgggggattg ctccctcggc cgaaccggag gcctatttat cctccctgca gccccggtac
    19801 ctgtatatcg cggatctcga gagcctccag ggcagaacac cccaggacaa cctcgtccgg
    19861 cggtgcgccg cccgggtcga gaagtgctac ctcgaccggg gctgccggtc gccagcggaa
    19921 tgcacggcag tcgtcggggt gacgccggtc gtcggcaccg agacggcggc gagagcgatc
    19981 gaagaccttg cggcatacga ggcaggctat ctcagcatcg acgtccagag gggccgggtg
    20041 ctcccctggg gcatccgacc ggcggagatg ctggcccgtg catcggccct ctcgtttgag
    20101 gggtgtatca tcctcaacat cggcgccgtg ggaacagagc ggggcctcgt ccaggaggac
    20161 ctcgaggaga tgcgggcatg ttaccccgga cgcctcctct acgggggcgg cgtcgccggg
    20221 atcgacgaca tccatctcct cgacgacgcc gggtttgacg gggtgatcgt cgcgaccgcc
    20281 gtccaccacg gcacggtgcc gatcgagtgg atccggagag gatacccgtg ctgatcacca
    20341 tcgagggcat cgacgggagc ggcaagagca cccttctcgc ccgtctccgg gaactgctcg
    20401 ccgacctcga ccccctcttc acccgcgagc ccggcgccac ctgggtcggg gactcggtgc
    20461 ggcgggcggt tgccgagcgg atggacccga tcaccgaggc gctgctcttc tgcgccgacc
    20521 acgccgcaca catcgatgcg gtgatccggc ccgcgctcga cgagggggaa ctcgtcatct
    20581 ccgaccgcta ctcggactcc cggtttgcct accagccggt cgtcctcgat ggcctcctcc
    20641 ccgacccgct cctctggctc cgccggatcc acgaaggatg gtcgatccgg ccggaccgga
    20701 cctttcttct ggtcctgccg gtagaggaag ctgtctccag gctcgatcct gcaaaaaaga
    20761 gagaatactt cgagaatgcc gggatcctcg cacgggttca ggagaattac ctgagcctcg
    20821 cagcctcgga tcccgcgcgg tttgtcatcg tcgatgcact gcttaaaaaa gaggaagtcg
    20881 cccggttcat cgccgacgag atcaggacga gtgaccgatc gtcacaatca cgtccccgag
    20941 cgtgaggacg tccacctctt ctcccgccac ccggtaggcg accttcggcg tgaaggagcc
    21001 gggcggcacc gggatctcct cgtcgccgat ggtgaccgtt gcgggcggat ttttgagcag
    21061 ctccggccca agggcctgca cggcctccat gaatccgcgg gcctgcttcc gtagcgtcgg
    21121 cccgatgatc gccatgttga agttcacgcc gctcacgacc tcctcaagtc ggggcgtgcc
    21181 ggcgctccac cgggcatcgg cggcgagcgc ccgcccggca tcgccggcgt cgtcgaccgg
    21241 ctcggatgcg tagatcatca cgtggccgag cggtgcgttc agtgccatgc ccttatcgtg
    21301 cttgtagcgc cggagttcgg cgaccgtctt cacgaggaga tcgccgtggc gccgcgcctc
    21361 atcgtcggca tacgcaaagt caggccaggt ctcggcgtgc acgcttcttg cggccaggtt
    21421 gtgatagcac tcttccgcga agtgcgggat gatcggggcg aggagacggc agaggacgtc
    21481 catggtcgtc cgaagtgcgc tgcatgcgct ggccctgctg ctgtcgtccg cgtacaggcg
    21541 acccttcacg atctcgacgt agtcgtcggc aagcgtgttc caggcaaact cccggatagc
    21601 cttgagcgcc cggtcgaact ggtagccctc catcgcggcg gtgacctccg cgacggtctc
    21661 ggagagccgg acgagcagcc accggtcgat gagttccgtc accggtgccg gctccccggt
    21721 caccgtctcc aggtgcccca tgacgaacct gaagatgttc cagagtttgg tctggaaacg
    21781 ggacgcagcg acgacatcgt tccagttgaa catgatatcc gagccgatcg ccgcgcctcc
    21841 cgcaccccac tgccgcagtg cgtccgcgcc gtactgcccg acgatctcct cgggggcgat
    21901 gatgttgctg cggctcttgc tcatcttgaa cccatcttcc ccgagcacca tgccgttgac
    21961 gaggatctcg tcccaggggt ggccgccgac cagcgccttc gcccggagga tcgtgtagaa
    22021 cgcccacgtc cgtatgatat cgtggccctg ggggcgaatc tgcgccggga agagcggggg
    22081 cttgccgctc ccgtcccatc cggtgacgtg gagcaccgat atcgacgagt ccatccaggt
    22141 gtcaagcacg tctttctcac cgatgaagtc ggacccaccg cacctcgggc aggggactct
    22201 gggcttatcg acggtcgggt ctatcgggag atccgcctcg gccgggagga ccacctcacc
    22261 gcaggtggtg cagaaccaga ccgggatcgg cgtcgcgaag atccgctgcc gggagatgca
    22321 ccagtcccac tccatcgagt tggcccagtt ctccatccgg gaaaacatat gctccggcgt
    22381 ccaggagatc tttttggccg catccaggat atcgtcccgg tgaacccgca cgaaccactg
    22441 gcgctccgag aggatctcga tcggggtctt gcaccgccag caggtcccta cccgctgctc
    22501 gagttcctcc tgccgtttca ggatcccggc ctcttccata tcgtggagga tcgccttcct
    22561 gcaggcctcc gacgacatcc ccgcataggg ccccgctgct gcggtcataa cgccctgacg
    22621 gtcgattgcc ttccggagtt ccaggtggtg ctgtttccac cagtagacgt ccgtcttgtc
    22681 cccgaacgta cagatcatca ccgcaccgct gccgaagccc gggtcgaccg cgacgtcctc
    22741 gatgaccggc acctcgtgct cgaagatcgg gaccgcgagc ctcctcccct tcatgccgcg
    22801 gtatcgctcg tcatcggggt gaaccgccac cgcaacacag gccgcgagca gttccggccg
    22861 ggtggaggcg atctcgatgc cgtcaaagtc gaagtagttg agcgtggtcg tgcgggggac
    22921 gtagttgacc tcggcaaatg caatggccgt ctcgcaccgg gtgcagaagt tcaccggatg
    22981 ctcgctctgg tagatgtcgc cggctttgag catctgcaga aacgaagcct gagtcttttt
    23041 ataatactcc gggagcatgg tgatgtattc gtggctccag tcggtggaga aaccgagccg
    23101 ccgcatggtc gtccgcatct tctcgatgtt tccgatcgtg aggtcgcggc acatctcccg
    23161 gaacttctcc ctcggtacat cgtttttggt gatcccgtaa gtctcctcga ccttcacctc
    23221 ggtggggagg ccgtggcagt cccacccctg cgggaacatc acgttaaagc cgcgcatacg
    23281 cttgtaccgc gcgataaagt cgatgtagca ccagttcagt gcgtttccga tatggaagtt
    23341 gccggtagga tacggcggcg gcgtgtcgat gatgaaccgg ggttttggcg agtcgggatt
    23401 gaaataattg tcctcatcgc gccaggtgct ctgccagcgc ctctccactt cttcgatatc
    23461 gtagttttta ggtagttgat gtgacggcga cattttcagc gtatgatctc tctctttcca
    23521 gtgaaatata ttgttctacg gggctttggc gccagcggat atcctgcgcc gtgcatgggt
    23581 ttgccgtccc tgatcggagc ctcatggaat gttcgtcctg cggaaactcc ggagacggca
    23641 ggcgttcgat cctaaaggtt ttttccctgc accgccaatc aagtatgaat gacaaagccg
    23701 caggggttgg ttctggcatc ggcgctcacc ctcgtcttta taagccttgc ccctctgctc
    23761 cagccagcat ggctgctcac gctctttctc attccactct cgctcgtcct ctttctcatc
    23821 agggataccc ggtatgtatc gctctcgatc atcgcacttg ccgtgctcta cgggctcggc
    23881 tggctctcga cgttcgtctt cacctgcacg ctcggcatcg tcgtgatcgg ggagctggca
    23941 ttccgcctta ccggggggaa gcaggcgtcg tatctctacc atctggttgc cgccatcggc
    24001 atatcgcttg ccgtgatgct ctacctcggg tatactgcgc cgctcgtcgt cgtgatgggc
    24061 gtggtcgccg cggtgctgct tcgtgcggtc ctccgggggc gggacgacgc cctgatgatc
    24121 gaggcgctcg gtgtcgcgat gaccatgttc ctcttcgagg aactcaactt cgaggtcgac
    24181 ctgaccatcc tcgtcgctgc ggccgcgata gcgttcgggt tcggctacat ctcttaccgg
    24241 ctccgggtgg ccgatatcag cggcctcttc tcggcagcca tgatcggcat catcctgatc
    24301 gtctttgcgg acatccggtg gttcctgatc atgctcacct tcttcatcat cggcgccggg
    24361 gctacccggt accgctacgc tgagaaggag caactcgggg tcgcccagga gcacgggggc
    24421 gtgcgcggct acttcaacgt ctttgcnnaa cggcctggtg gcgaccggcg ccgccatcct
    24481 ctacggtgta accgggcacc cggcgtttgt ggcgctcttc atggggagcg tcgcctccgc
    24541 cgccgccgat accgccgcaa gcgagatcgg ggtcacgggg aaggtgccgt acctgatcac
    24601 gacgctccgg ccggtttccc ggggaacaaa cgggggggtg accctgcggg gcgaggcggc
    24661 ggccgtcgtc gcatccgtcc tcgtcgccgg cgctgcctgg gtgatgggcg tcgccgaccc
    24721 ctggatggtc gtcgtcacgg tcatcgccgg gttcatcggc acgaacgtag acagcctcgt
    24781 gggcgcaacg ctcgagaaca gcggcagggt cgggaactca ggcacaaacc tggcggccac
    24841 gttcttcggc ggggtctcgg ggatgctgat ctatctcctg cgatgatgtt gtggccgggc
    24901 gcaccggctc cgctacgctt tttgagatgg ttctttatcc aggtcgactg gcaggttccc
    24961 gtgcggggtc atggggcagg gagttatatc tctaaacatc tgtaaccggg agggggacac
    25021 cccctcccg
//
LOCUS       DAJB01000038           10824 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_88716, whole genome shotgun sequence.
ACCESSION   DAJB01000038 DAJB01000000
VERSION     DAJB01000038.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_88716"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1966 a         3577 c         3473 g         1806 t
ORIGIN      
        1 cggccccaaa gaggatggca tctgccgacc ggagctcggc catcgtctcc tctccgcagg
       61 cgctcccggt ccgctcccag agcccgtatc ccacctcgac gtcgaagaac tcgaagtccg
      121 ggcgtacggc tgcgaggatc tcgcgggcga ccgggacgac ctcatggccg atgccgtcgc
      181 cttcaacgac cgctaccttc gtcaccctgt tccctccatg cggccgtgac gcgccagacc
      241 gcctctgcaa tgtcccggga cgacgcgccc cagtggacca ccgcaccaaa ccggccgttc
      301 cagcgctcta taccagcccg ctcgctccgg cagcaccgga cgatgaaggg ctcgctttcc
      361 accgcctcga gcgggcagat gctctccacc ataatccctt cgccgtaaca ctcccgcacc
      421 aggtctcttg ccgtctggca tcccgcgacc cggtcgcccg cccggagcgt gtccgcgtca
      481 agggtgcgag ggaagccggc cgcatggcag gggtagacgt cggcttcgat ctcccggata
      541 tccctgatat ggtgctcgaa gatgacctcg aggtctccaa agagcccggt cttctcaagg
      601 tcaagaagcg tggccgagag gtgggggcgg ggcggagtga tgtcgtagac atggatgcgg
      661 aggaaacccg agaggtccgg atcgagcacg aacgtggtct gttcgccgtg gccggtgaat
      721 atcgtgcagc ggtgtccgga tcgcatcgcc gcccctacta agaatgttcg atcctggaca
      781 ttcactcgat ccgggtagcg gtagacctcg tcgccggtgg cgagcacccg ggttgagagc
      841 accctgcgca tcaacccggt cccgtccggg tcggtctcca cctcgagcac ctccgtgccg
      901 tcggcggtct cccggaccag gtagcgcgag aggaagtagg cccggttccc gtacggtgtg
      961 ctggtcgcgg acccaacata tttgcaatgg ggagggaaga tcaacggcac gacctccagt
     1021 agggaaccag ccctccggcc cggaggatgg cgaccatctt ctccgagagc gggcgggcgg
     1081 gatagcgctc cccgtccacc tccacccacc cggcatcgag gtcgaatgct acgcgagcgc
     1141 cgtcggtgca ggagaccgcg gcctcgatca ccgggaggcc gacgttgacg gcgtttctaa
     1201 agaagatgcg ggcgaatgac ggggcgatga cccccaccac cccggcctcc cggagggcga
     1261 ccaccgcctg ctcccgggac gacccgcacc ccatgtttct cccggcgatg atgattgcgc
     1321 cggaaagacg gccggcgagc gttgggtcca ggtcttcgaa ggcgtgctcc gcccagaccg
     1381 agcggtcctt tgtccggagg tagcgcccgg cgatgatgat gtcggtgtcg atgtcgttgc
     1441 cgagacagac ggccggtccg gctccctgca ttataccccc tccggcacgg cgattacgcc
     1501 ggctatggcg ctcgcggcgg cagtggcaac cgacgagagg tagatctcac cgccgacgcc
     1561 catccggttc ttgaaattcc ggttggcggt ggagaggcag acttcgccct ccccgagcac
     1621 gccggcatgc gccccaaggc acggcccgca cccgggcggc gccaccatgc accccgcatc
     1681 cacgatatcg gcgaggacac cggtggcgat cgcccgggca agcaccgccc gcgaggcggg
     1741 gacgaccagg gtgcggaccg caaccttcct cccccggacg atgcgggcaa accgggcaag
     1801 atcttcgtag cggccgttcg tgcaggtccc gacgaagacc tgatcgaggt gcgtgcccgc
     1861 gacctcctcc gcctcccgga cggtatccac gcgatgcggg accgcgacga cgggcacgat
     1921 gtcggcgagg tcgatatcga cggttcgctc gtaacggcag tcctccggtg tctgggacgc
     1981 cgcggcaagc ccgtagtccg caaggtggcg gacggtgacc gcatcggcgt ggaacatccc
     2041 cgccttcgcc ccggtctcga ccgccatgtt gcagagggtc agccgtccgt ccatggagat
     2101 tcctgccgcc ccgtcgccca cgaactccag cgcccggtag gtcgctcctt ccatcccgag
     2161 ctctgcgacg taggtgaggg cgatgtcttt tggctccgcg gcgcccgtaa gcccgccgga
     2221 gaacctgacc gcgatcgtct ccgggacgcg gaaccacatg gctcccgacg cccagaccgc
     2281 cgccatatcc gtcgctccca ccccggtggc aaacgcgccg aaggcgccga gggtgcaggt
     2341 atgggagtcg gcacccacga cgaccatccc gggccggacg accccttcgc tcatcacctg
     2401 gtggcagatt ccccctccgg catccgagag ttctatcccc gcggaccggg catacccgcg
     2461 gagctccgcc tggagcgtcg ccgtcgtccc ggtgtttgcc gggacgatat ggtcgaagat
     2521 caggcgcagg cgctcggggt gggggagccg ccccacgccc atctcgcgga gtgcctcccg
     2581 gacaaggacg ccggttccgt cgtgggcaaa ggcgatatcg acctcgcggt ccacgtattc
     2641 ccccgccggt gcgccgagga tccgctcgga gagcgtgctc acgcacgcat cccctccttt
     2701 gcctgcctga tgatcccgca gagaatctcg ggggtgacgc tgcacttccc ctcgctcttc
     2761 tgtttgacct gctcaagcac ccaccgcgcc tcctcatcgg agaggtcgaa cccgtaggcc
     2821 ttcgcgacat gctcgagcgc ccgcttcccc gtatgcttcc cgagtacgaa ccgccgctcg
     2881 ccgcccacca gctccggggg gatatactcg taggtcgagg ggtctccaag gatggcggcg
     2941 atgtggatcc cgctctcgtg ggcaaacgcg ttctcgccga cgatcggccg ggtacgctcc
     3001 gggaccacgc ccgagcaccg ggcgaccagc cgcgagatct cctgcaggcg ggagaggtcg
     3061 tagcggtcga cgccgccctt catccgcagg gcgacgagca cctgctcgag cgccgcgttc
     3121 ccggcacgct cgccgatgcc gttgacggtg gtgtggagct ggaacgcccc ggcggccgcg
     3181 gccgtgatgg tgttggccgc cgcaaacccg aggtcgttgt ggcagtggat gcagagcggg
     3241 aagggggcag catcgaggat ccgggagaca accgcatgga cctcaagcgg cgtgaggcac
     3301 ccgaccgtat ccgcaaaact gacgatatca gcaccgcatt cgacgccccg ggtatacatc
     3361 tccaccagaa acgccgggtc ggtccgcgag gcgtcctcgg cagcgaatcg cacctgcagg
     3421 ccgtggtcgg cggcgaactc caccatgtcg agggcggcgt caagcacctc ttcacggggc
     3481 ttgcggtact tgtgccggat gtgcaggtcc gaggtggcga tgaagatgct gaccatatcc
     3541 acgtcgcagt cgagcgccgc ctcgacatcg ggctggagcg cccgtgccag gcagcagacc
     3601 cgggcgtcaa ggccgctgtg cgcgatggcg gcgacacact ccttctctgc cgccgatacc
     3661 accggaaacc cggcctcgat cacctccaca ccgatctcgt cgagcgatcg ggcgatcgcc
     3721 gtcttctcat cacaggtgaa cgagacgccg ggagtctgct ccccgtctcg cagcgttaca
     3781 tcacagatct cgatatgcca cggtttcatg gattccacct tcagggcaag tgccttcgat
     3841 caccaccgtc cggggccccc cttgcggggg gacgaggacg cggctaagcg tcccgacgtc
     3901 gtctgcggcg cagacggccg gatcggccca ggcgatgtag cgaaggatat cagggacggg
     3961 gtgaccgccg gtcatcccca tccgggtgtt cgcgaggacg atacagagga gcgggagttt
     4021 gcgttcgtag acgtcgatga gggcgtttag ccccgagtgc aggagcgcgt agtcaccggt
     4081 gagcgcgacg ccggggccgg tcgccgccac ggcgaccgac gagccgaggc cgtaggtgac
     4141 gatgccgatg cggtagggcg ggttcatcgc gaggatcgcg cacccggcat cgcagaccgc
     4201 ccgcattgag cgctcgcgga ggatctcaaa cgccgggtgg aacgggcagt cccggcagaa
     4261 cgtccggcag tagccccggg aagagaaccg ttccggctcc ccggcggggt cgggaggggc
     4321 gaggagccgg tgctcccgga ggaaggggcg gccgacctcg cgggtcaggg cgagcacgtc
     4381 agggcccgcc gggggcgggt agacggtgac tgcacggtgc tcgtcacccg agaaccggtt
     4441 caacggcgag gaccgcgacc aggcaaacat tgccgcggtc cggcggtcgc tcgccagcgc
     4501 ccgtccggcc atcgtgagtg cggggtccgc aaggcttccc tcccggtcgc tccggggaga
     4561 gaggggtggt tccggcacgt ccgcgtccag gaggtcgggc gtcaccctga cgatggccac
     4621 ccgcgagaag gtctccgaag cctcaaacgc tgcaacgatt gccggaccga tcgtctcgcc
     4681 gtcgggttcc agcaccggaa cccgggctat ctcgccgtag tagcgggagt cctgcgtgac
     4741 gtccgatgcg accgggcgca cgtcgtcgcc gacgaccacc acgacgcctg cccggaggcc
     4801 ctgcgcagtc gcatgcacga gcgggtctgc acaggcgttg aggccgacgt gcttgaccac
     4861 gaccgcggcc ctccgcccgg agagcgagtc gccgagggcg tactcgagcg ccaccttctc
     4921 gtttacggtg atcgcggccc cggccgaggc ggcaacctcc gagaccgggt agccggggac
     4981 ggcgtagcag cggtcggcag accggaggat ggcgagagcc agcgcctcag tgcccttcat
     5041 gtatccctcc ccggcaccag gtcgcacccg gtgcatgccg aacacatcgt cagcgcccgg
     5101 cgggggtcga gccctgcctg ccggaggata cgctcatggg tctcgcagag cgagagcagc
     5161 cggtcggcgg acggtcgggg ccggtcggca agagacgccg ccggggtgag cggacggagg
     5221 accgggatga ccccgatcgc tgcgagatcg cccatgaccc gctccaggtc tccatcggtc
     5281 tccccgaggc cgacgatgac atttgagtag accctgtccc tcccgaagag cgccacggag
     5341 gactgcaacg cttcccagac tgttctccac gagagaccgg ggcacatctc tgcaaagagc
     5401 gcctccgtcg ccgcttcgat gttgaacttc acctcgacga cgccgagagc gtgaagccgg
     5461 gccggggtcc cggggcgggg gtagatcgag accccgatgg gaagtgcttg cttgaatggg
     5521 cggagcgccg ccacgacgtc gaggacatac gcctcctcct cctcgatcga ggaggcgacg
     5581 ccgctcgtga tcgcgatggc gtcgatccgg tcggccacgc tcccgaccat ctccacgatc
     5641 tcggcgaccc ccttccgccg cccggggaga cccggcacct ggcagtagcg gcagtggaag
     5701 atacaggtcc cgctgaccgt gatataggcc tggcgggggc agtggagggt agcgggctcg
     5761 agacgaccgc tgatctgccg gtcgtcgatg atgaggtcta cttcgcctcc tccacggtga
     5821 acgacctcga tgggacctgt cctgtcgatg ccgacccgga cacgccggcc gccggtggag
     5881 aagaagagcg agccggacgt tcccgccgag gggccggcag ctgaacggga tatatacggg
     5941 tctgcaggtt cgccggagag gcgaaccgaa ccagcctcga gcagccgggc cttcagccga
     6001 ctccattcca taacgccagc gcctcctcat accgtgtggc gaacgggatc gccgccaccg
     6061 ccccgatcgc accccggccg ttcatgacga ccgagagccg ccggtcggtg agagcggcaa
     6121 ggtcgcccgc agagacctcg ccccgcttta cgcgccgccc aaactcctca agcggggcgg
     6181 aatcaaaccc gcgccagacc gccatcccgg tctcatccga gagcgtatac tcctccagat
     6241 agtcgcggta gtgccggacc agtccgacgg ggtcggaggt ggcgaactcg caggcgatgg
     6301 cgacgcaatt tttggtccgg tagggaacgg gaaagagctg gacgatggtg tgggagaggt
     6361 agcgggaacg gtcgtcctcg acggcccgtg cgatgttgtg ggccagcgtc caggtggcgc
     6421 cctcctcagg ggtatcggtg tcgtcgaccc cgatgatcac ccgctcgcgc cgggggagcc
     6481 agatggtcga ccctgcgagg cgcccgcccc cggcagggtc gctctcgtag cggatgacgc
     6541 cgcgggcctc ggcgcggcag atggatgccc ccacaccgcc gccgccgaga ccccggtagg
     6601 tgacggcgat ctcctctcct tcgacggcga caccggcgat ccccgccgga aagaccgagc
     6661 cttcgagcgc gaggtcggcg gtgccggtgc ggaggaggta gcggttggtc gcgcccacgg
     6721 tccggacgga gcggacgagc gggctctgcg catagtggcg cttcacccac atcgccccgc
     6781 cgacgcagtc gaagaactcg atgagttcta catgctcgcc gctctcgtcg gccaccgcca
     6841 cgatctgtgg ataccgtatt gtgtaaggat cagagagttt ctccatagag acctgccgct
     6901 tcaacgccct tgttccccca gagcagggcg ccgagcgccc ctatccggcc cttccgcccg
     6961 gtcgagtcga tgaaccggac acccatccgt gcggcctccg cttccgcgtc atcgaccgtc
     7021 aggagttcag tcttgaccct tttaaggtaa ggagactcct cggcaaacgc aatcccccgg
     7081 tagacggcga tgccggtatc gttgctgacc gcccccgact cgacgaagtc gcggacgtac
     7141 tcgaggagcg cgtccacctt tccggggcgg acggcgaagt ttaaggcgga cccgacacag
     7201 ttggtggtct tcttcgggac cgccgggttc aactgcacga ggcgcatgtc caggtactcg
     7261 acccccggga tcgtgcaggc ctcagcgcac ttgagtgcga gcacccatgt ggcgccttcc
     7321 tcgcgggtgt cggtgtcgtc gatcccgatc gtcaccttct catacagcgg cgatacgatc
     7381 cggacccggc agacgcgggc gccgccgatc cgcagatcct cctcggacgg gtactcggcc
     7441 cgggtcaccc ccggcgcctg cggcaggcat gcggcgacgc cgacgcctgc tcccgcgatc
     7501 ccggaccagg tcgtcacgac ttcgtccccg acaacctcga caccctcgag cgcctgcccg
     7561 ccgatctcct taccggcagc cccgaagttc gccggcgata cacccaggcg ggcgagcatc
     7621 gtcatcttcg tgccctctac gtggcaggac tggatcgcac ctttcgcccg cacccggttt
     7681 gccgcatccc actcgatggt cccccgggcc ctgcaatgct cgcggatctc tgcgagaccg
     7741 gcgttatggt cgaccatgac gaggtatttc atcgagaaca acggcccgaa ccgggcccgg
     7801 acttcatcag gcgtgagcgt aatcatgatg tcaccggaat tatcgttaac aaaattatcg
     7861 ttgataacgg tatttaatgg gatcgatcat gagggttgga acggcgggga ccgggctttg
     7921 gcgggggggt ggccggtgag ggaggctcat atacgtgcat gggttcctcg atacgaacaa
     7981 tatccagcag cagcccgaac gtgaggctca tatacgtgca tgggttcccc gccgcaggcg
     8041 gcgggtggaa acgtgggagg tcaatcggat tttaatttcg gacgttcggt ccatcgtgcc
     8101 gtcggtcggt tgtcactcac accttcggtg ctcgaactcc tgccctgcag ccagtcgtac
     8161 tcacacatcg ttcttgggac atcgcttacc ccggtgcacg gatttcgtgg ggatatcgcc
     8221 acgcactgcc cccgcccttg gggcggggga ggagcgcgaa gcgcgggtgg ggtggggctg
     8281 atgagatgtg tggatcagag cgagatggag acaggggagg gggagacccc ctccccgtca
     8341 gccccacccc caatggcgat agccaccacg gtccactgca tgaggacatg cccgataaaa
     8401 aggggcgccc cagggacaag ccgggtccgg caccagcctc gtcagggtaa ggatgaagat
     8461 cagaacaggg tttcaacaga gcgcgctatg agccggaagc tcatagatag atgacaatga
     8521 aaaacagtga tgaccgaccc gccggttctg gcagatgctc tgcatacttg caggatgttt
     8581 ttcataccag ccctaggttg gaaacctccg aggcattcga acgctcccac ccgctcgatc
     8641 cccgatcctc acaccctcaa caacagggcc aaccagacgg caccctcaag aaccgccaca
     8701 gaaaaaagag attaaaggta gtccgcgagg acatccttca ggtcgaactt gaacggcccg
     8761 aggctgccgc cgaagttccc ggcggtgatg aacttcacgc ccggcaccct ggttgccgca
     8821 cggatgccct cggccatcgc ggtcttgatc gagtcctggt cgacgccgtc gatgacgatc
     8881 tcgtagaccg ccgtgacgcc ctcgggaacc ttgctgccct cgaccttctc tctcagcgcc
     8941 gggcagtagg cctcgttggt ggttgcaacc atgaacttgt acttcttgct cccgaccttc
     9001 gagccgctcg cgacgatgcc gccggggaag ggcgtgatga caccgcagac gccactgatg
     9061 gcgtcgaccg ccgcctgggc ggccgtaaga gcggccatct ggttctcgcc catgatgaag
     9121 aagttgccgc ccgctacgcc cttgacggcg ccgaactcct cctcaccgat atactcgccc
     9181 tccatgatcg ggatagccca taccgtcctg ccgccgacct cgcgcttctc ctcgaacccg
     9241 tcgccgaaga agtggagctt gaccgggagc ttctcgggga cctcggcaac gacgtcggag
     9301 aggccgtcaa agactgctgt cgtcggggcg gtgagaatgc actcggcgag acgctcaacg
     9361 acctgctctt tgagcttctt cttgctcgca cagatcagga tcgcgtagcc gggccgtccg
     9421 tcgggggtct cgtcaccggg gacgaagcag tcgatacctg cctccgccgg acatccgatg
     9481 gtggaggtgg caaagccgac ggcctccact gctgccttgt atgcccactc ctcggtgacg
     9541 gcggtgataa tgggccgtgc cacccaggtc gggaacgctt ctgcataggt atcatcgata
     9601 gtaacgccat tcagttccat ggacagaata cacctcgttt gctacaatgt tacgatgcgg
     9661 acagaataag gtttcttttt ttcccgggcg tgcgcacccc gcgctcccgc gtgccatggg
     9721 ggattcaaac tgcgcggccc cctcnnctcg ccccggttgc accttccagg cgcaaaacgg
     9781 ccatacagcc catgcctgca gcagggaccg ggttaagcat gctgcaggtt gccagatccg
     9841 cccggcgggg ctatcctgga tgcggcaggg ggaccgcggg ccaggatccg gacggtctct
     9901 tccagacggg ccgaggcacc cggcgcggag agaggagaac cgatggatgc agaaatggtt
     9961 actacctaaa ccgcgcaacc cgtgacaggt tcctgccgga aggagaagag ggaatatacc
    10021 tgactcgcga ggttatccgg cctgatctca acactgccgg gcaggattgg ccaccgattt
    10081 tcaccagaaa cagggatttc tgatttaaca gtttcgtttt gcccttacgg caggaagtga
    10141 atttagcagt gcgattttga acatcgggtt aacataacaa ctattttaat ttataaaatt
    10201 tgacagataa tcatagttct ggaatataag actgccgaat ttcagaaagt ttatgtatat
    10261 tctttgacta ttctgtttat atcgattttt aatcgatcag atctggtgcg tgataccatg
    10321 gcatttgcat tgcacatcaa tatggaacga tgtacaggct gcaataactg cgtggtggca
    10381 tgtccggtcg acgctctcga gctctacacc gaagatccgg taaccaagga gaaaatttac
    10441 aaggtcaaag acggcaaagc cgtcatcctt gactttaact ccgagctctg cgccgggtgc
    10501 ggcgtatgcg tccaggcatg cccttacgga gttatcaagc ttgaaggacc gtgggagagc
    10561 cgggtgaaag cccgaaaagt ggaagcctag gagtgaaact agaatatggc attgtttcca
    10621 aagtactcga aaacgcggga aggacagaac gtcattatgg agcagaggct cctgaaggcg
    10681 gttaacaacc tgatcctgaa cgcagagacc tgcacgggct gcggcatctg tgtcgacgcc
    10741 tgccctgaag aggcaatcgt gctcgggcct gtcggtgcga cacgacgcgg ggcaatcgac
    10801 tacgctgaac ctgtcgacgt caac
//
LOCUS       DAJB01000039            4175 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_77201, whole genome shotgun sequence.
ACCESSION   DAJB01000039 DAJB01000000
VERSION     DAJB01000039.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_77201"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          856 a         1217 c         1277 g          825 t
ORIGIN      
        1 ggggcagtca tggcgatacc cggtggaaag ccgtgctcca gagtgcgacc atcgggagag
       61 acggatccgg gttcaacaaa gccgaaagtt ccctgttttg tgcgctcctt cctcgtgccc
      121 atcgtgcact cctgcgtgag gtcatagcgc ccgcccccac acgcaaaaat tcctgaaata
      181 aagtggcagg atgcaccagg tgcacagccc ggtggaaagc cgtgctccgg agtacctccg
      241 gagtgcgacc atcttgagca tgcagacagg gtttcagcaa agcctgaaat agttataccc
      301 gggtaagacg tgggtttatc tcctgagccc gggcgtaaca acgatccgcc tcatcgggtt
      361 ttcccagact cgacagtatc atccccttca tccaccaggc cgtagcatac tccggatcaa
      421 gctcaagagc cctgtctacg gattggagcg catcctgata ccttctcaga ttgtagagtg
      481 cgacactttt acccagccag gcatcttcaa ggaaagacca tatgcccaca cctgaccgtt
      541 ccctcgaaac taccgttggt ccgcttacgc tcaagaaccc gttcctgctt gcctcgggtc
      601 cgccgaccgc ttcgggcgaa cagatccggc acgcgttccg gctcggctgg gcgggtgcgg
      661 tcacgaagac catcgtcccg gacgcgatgg agatctcgca gacaagcact ttgcgggcgt
      721 tgcggcgctc aagggctgcg cccttccccg catcggaacg catgcatccc tctcccgcag
      781 tgtccaccgt ttcgccgcca tgggattccc gaaacggtgc acgtcctgcg gccgctgtgt
      841 aaccgcctgt cgtgacgggg ggtatcacgc catctcgata gcagaccggc atgtcgtgat
      901 cgatagagac ctctgcaacg ggtgcagcct ctgctcccat gtctgtcccg aaagcgcgat
      961 cgtcatgctg ccgggtgcgt gaaacttgaa aggctccgtt gtgcgaacga acatcaatgg
     1021 gtgcaagttg gagacagggg agggagcgag ccccctcccc gtcagcccca cccccaatgg
     1081 ggatagccac cacgatccac tgcatgggga tatgcccgag aaaaaaggtc gtcccaggga
     1141 cgggcagggg tccgggcacc agcctcatca gggtaaggat gaagatcaga acagggtttc
     1201 aacagagcca aaagaactat tattcgggaa aaagggtggc cttcaggcga acatctcgcc
     1261 gaaggtggtg tggaagtggt gctggtcgaa gtccaccgcg agtttatcga tcgccttcat
     1321 gaagtcgtcg ctgttgatgg tatcgcgctc catcctgatg gcgaacatgc ccgcttccat
     1381 gcatattgcc cggaggtcgg cgccgttctt cccttccgtg agccgggaga cctccgagag
     1441 gttgacgtcc tcgccaaggc tcatgcgcag ggtgtggatc ttcaagatgg caagcctccc
     1501 ctggtggtcg gggagcggga tctcgatcat ccggtcgaac cggcccgggc ggaggagagc
     1561 gcggtcgagg atatcgatcc ggttcgtcgc cgcgatgatc ttgacgtcgc cgcggttatc
     1621 gaacccgtcc atctccgcca gaagctgcat cagcgtccgc tggacttcac ggtccccgga
     1681 cgtcgtcgag tcgttccggt gcgcgccgat cgcatcgatc tcgtcgatga agatgatgga
     1741 cggcgacttc tgtttcgcga ggtcgaagag ttcccggacc aggcgggccc cctcgccgat
     1801 gtacttctgc accagctccg agccgaccac gcggaggaag tgcgcgttcg tctggtgggc
     1861 caccgcccgt gcgaggagtg tctttcccgt gccgggcggc ccgtgaagca gcacgccctt
     1921 tggcggggag atgccgatct tctcgaagag ttgcggcttt gtcagcggga gctcgaccgc
     1981 ttccctgacc tcctcgatct gcggctcaag gccgccgatg ttctcgtaag tctcctcagg
     2041 agactcgacc agttccatgc cgtagatctg cgcgtcgtag cttgtgggga gcacatccac
     2101 gatggcaagg gactgctggt tgagcgtgca tcgtacaccc ggtttgagga gatcggggtc
     2161 gataagttgc gacgtacgga ccaggaaccg tgggcctgcg ctgctccgca caatcacccg
     2221 gctgttgtcg ataacgtcgg taaccgtccc gatcacgaga ggagggctgc ggagctgctc
     2281 gatctcactc ttcagttttc gcagctcgcg ctcgtagcgg atcttctggg tctcaacata
     2341 tcgtttctcg gactccatct ggcgaaattg ttcgcgtagc tcgagatttc ggttttcgag
     2401 gttcgttatg cgttccagga gatactggta gatgtcttca ccggtatcct tctctggtgc
     2461 ctgccgagcg atatctccca tgggtctcct cgattaatta tataggttaa aatgaatata
     2521 aaattgtttg cgagacttat gcagtgcgaa ctatgcggcg ctcctatagt gggaccgtcg
     2581 aaaaccatcc agatcgaggg cgcagaactc tgcgtctgcg ttcgatgcgc aaaatacggt
     2641 acagaagtcc agcaaccgcg acggaaaggt gccgcgcagg caaagccggg actcgccgcc
     2701 ccacaggcaa aaagaaggcc gcgggacgtc tttgacctga tggaaggaga gatcgtcgac
     2761 gattacgccg accggatccg ggccgcccgc gaagagaaag aatggtctgc tctcgacctt
     2821 gccagggcga tcaaggagcg tgagatcctg atcaaaaaga tcgagaagag agatctcatc
     2881 cccgaagacg aagtccggcg gaagctcgag aaagcgctca atatcaggct gatcgatgcg
     2941 cccgaagaca gcatatccgc cagtgggccg ggacgggtca ccatgaccgt cggcgacgtc
     3001 atatcgttca agaagagccg gaaataaggc aatccccata cttttttcgc gggaggccgg
     3061 ccttattccg gatccacttc acgcacgata atccggcata cttttataca agagcgcgta
     3121 acggtatacg caatgtgtgg tgaatgtttt ttcgccggtg aacgttttta cctcggctaa
     3181 cgtagtaacc atcacccgca cagaagcaac cagccagttt ttgaccgccg atatccgtga
     3241 ctacggaacg gcagttttct atcgttattc tgcccggttt acctgcttta cccggtttac
     3301 ctggtattaa acattcccgg agactgtact cactcaaatc cgggaggact gtggttatga
     3361 gaggaaaaga gattcgtctg gaacgtatca tggatcgaaa taccggcagg accatcatcg
     3421 tgccgcttga ccacggcgtg acggtcggcc ccatcccggg gctggtcgac gtcggaagga
     3481 ccatcgacct cgtcgccgaa gggggagcga acgcggtgat cggccacgtg gggcttgcgc
     3541 tgcacgggca ccgggggcat gggcgggacg tcggcctgat catgcatctc tcggcgagca
     3601 ccagcatcgg cccggacccg aacgacaaag tgctcgtgaa caccgtcacg aacgccttaa
     3661 agatgggcgc cgacggcgtc tcggtgcata tcaacgtcgg ggcggactcc gaagcgagga
     3721 tgctcgaaga cctgggcagc gtcgcggtcg cgtgcatgga ctggggcatg ccgctcctcg
     3781 cgatgatgta cccgcggggc aggaaggttg ccgacgagca tgacctcgag agcgtcaaac
     3841 tctcggcgcg ggtggcggcg gagctcggcg ccgatatcgt caagaccgtc tacaccgggg
     3901 acgcggactc attccgggag gtgacggggg gatgcccggt gccggtcgtg gtcgcgggag
     3961 gatcgaagac cgacgatcgc gcgatcctcg acctggtgga gggcgcgatg gaagggggtg
     4021 ccgcgggcat atcgatcggg aggaacgcct tccagcattc ggcgcccgac cgcctgatcc
     4081 gtgccgctgc gcttatcatc catgaagggc ggtcggcaga ggaagcgatg gagattctcc
     4141 ggacgtgaga atgtcaacta cccccggttg aaacc
//
LOCUS       DAJB01000040           14370 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_60015, whole genome shotgun sequence.
ACCESSION   DAJB01000040 DAJB01000000
VERSION     DAJB01000040.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14370)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_60015"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2918 a         4263 c         4478 g         2709 t
ORIGIN      
        1 cctaattatt atccgcgtgt tcctgtctcc cgataaagac gatcgcgatc gtcagcacga
       61 ggatggcgac aagcgtcatg ccgatgtcga cgggctcgac gggctcgatc ccgaaggtca
      121 gcacccgccg caccatcgcg gtgatgcccg cgatcaggat cggtgtcacc agcaccctgt
      181 tggtcctgaa gtatgccgtc acggtctcca ggatctctac gatgatgagg gtcaggagga
      241 gcgcgtgcag cacctggagc acgccctgtg tcatatcctc ggtatggatg agcgctgcta
      301 cctgcaggat aacatcgtag aaggagaaga tcgcgagcag ggagagggag gcggcgatga
      361 gcaggtatac gcccagtgtc acccatgata tggcggatat gaccgcatcg gcacggtttt
      421 tcaagggact catgtgggca ccctgtccgg gagaatatac atatgaacag gatattctga
      481 gggcattaaa cattccggta ggcctgcaac gggtggcgta ttgaaaagtt tatatcgtta
      541 gactgcgaat gatatgatac gcctgcaagt aaattattta tactattctc gcgttgtaat
      601 aatgctggat ggcagtacga gcggggtcta tagctcagtt aggtagagcg cctggctttt
      661 aaccaggtgg tcgggggttc aaatcccctt gggcccgttg ccatgggcga tgtctatgga
      721 ttttaacaag gtgatgcatc tgatacgcaa ctttttcgtc ggtgaggtgt agggcgatgg
      781 gcacttcact tgacgtactt agccatgaga tggttccgga ccatcagatt atggacggag
      841 aagaggtcac gaaccttctt gcgacgtacc atatctcttt agagcaactg ccaaaaatgt
      901 atcatgacga tcctgcagta aaggctatcg ggggcgatgt cgggaacgtc atccggatca
      961 ctcgagacag tcgcacggca ggtagagccg aagcctaccg gctcgttgtg aagagaccaa
     1021 agaaataatt cagaggccgg gagctgatcc atctgttaga cagaagtgtt ttgtcgagag
     1081 catatttttc acgggagcat gtagcgcgcc accagttaga ctcttataat aacttcctcc
     1141 tgtacaacct tcagaaagtc gtcgacgagc aacgtgttat cgagaccgat attgagaccc
     1201 gtgggaaggg aaaagaggcc gtgtgggttg aactgggcaa gatcgaggtg aaaaaacccc
     1261 gtgtccgtga ggcggacggg tcgcagtccg agctcttccc gagcgaggcg cgcctccgga
     1321 acctcacgta tgcggcaccc attcaactcg atatgacact catccagggc gaagagccgc
     1381 aggatccgat cgtcaccatc atcgggcagc tgccggtgat ggtgggttcg gccgcctgca
     1441 acctctacaa catgaacgac accgagcgga ccgagcatgg cgaagatccc ctcgaccccg
     1501 gcgcctactt catcgtcaac ggcacagagc gggtgctgat gaccctcgaa gaccttgcct
     1561 ccaacaagat catgaccgag ttcaccgagc gctacaacga gcggatctac gttgccaaag
     1621 tcttctcgca gtaccggggc taccgggcgc tcgtgatcgt cgagcggaac cgcaagaacc
     1681 tgctcgaggt ctcgttcccc tcggtcgccg ggcacctccg tttcgttgac ctgatgcggg
     1741 cgctggggct ccagggcgac cacgacatcg tggaagcggt ctcgaccgac gaggagatcc
     1801 tcacgttcat gatgcagaac ctggaagaga gcgagtgcga caccgtcgac gacggcgtca
     1861 tgtatgtcgg gaaaaaactc gctcccaacc agacccggga ctaccagcgc aagagggcgg
     1921 agtttgtcct cgacaactac ctccttccgc acttgaacta cctgatgccg gtgggcttga
     1981 aagaggaaga ccccacatac cgtgcgacag tcgagggcgt ccgcctcgca aaggcgcact
     2041 tcctcggccg catggccgag gcatgtttcg acctggtgct ggaccggcgc cggatcgacg
     2101 ataaggacca ctacgcgaac aagcggctga aactcgctgg cgacctgatg gaagacctct
     2161 tccggatctc gctcaaccgc ttaacacggg acgtgaagta tcagctcgag cgggccagca
     2221 tgcgccaccg cgacctctca ataggcaccg ccgtgcgcgc ggacgtcctg accgaacggc
     2281 tgctgcatcc acttgccacc ggcaactggg tgggcgggcg caccggtgtc tcgcagctcc
     2341 tggaccgggt ggaccacatg gcggtgctct cgcatctccg gcgtgtcatc tccccgctct
     2401 cccgctcgca gcctcacttc gaggcccgtg acctgcaccc cacccaatgg ggtcggatct
     2461 gtccgagcga gacccctgag ggtccgaact gtggactggt gaagaacttc gcccagatgg
     2521 ttgagatcag caagggcgtt atcaacgaag aggaagtgaa aaatattctg tacgatatgg
     2581 gcgtcatcgc cctccggaga gagacggcat gaggcagtca cgtgtcttcg ttgacggagc
     2641 tcttatcggg ctcgtggatg acccccggga cctggtgggg cggatgcgcg atatgcgccg
     2701 gcgcggcgag atccccgccg aggtcaacgt ctcctacaag gaattcaaca acgatgtcat
     2761 cgtccacacc gatcgcggca gagcgcgccg gccactgatc gtggtccgcg aaggcaaacc
     2821 gcaggtgacc actgacgata tcgaacacct ccgaaacggc gagatgacct tcgcggacgt
     2881 agcgcggaaa ggtgcgatcg agtttgtcga tgccgaagag gaggaagatc tcttcatcgc
     2941 tatcgacgaa gcggatctca cccccgagca cacgcatctc gagatcgacc cctcgctcat
     3001 cctgggtatc ggtgcggcgc acgttccgtt ccccgagcac aacgccagtc ctcgtgtcac
     3061 catgggtgcc gggatggtca agcaggcgct cgggttcggt tctgcaaaca tgaaactccg
     3121 ccccgacacg cggggacaca tgctccacta cgtccagaag ccgctcacct tcacccagac
     3181 ctccgatgcg atcggttccg acgaccggcc tgcaggccag aatttcgtcg tcgcgatcat
     3241 ctcctatgag ggatttaaca ttgaagatgc gctgatcatc aacaaggcct ccatcgaccg
     3301 gggcctcgga cgctcgcact tcttccgcac ctacgacggc gaggagcggc gctaccccgg
     3361 cggccaggtc gacaagatcc aggtcccgga cgaagaggtc tccggcgcgc acggttccga
     3421 gttctacgcg aaccttgacg tcgacggcgt catcaacccc gagacggttg tccgcgagaa
     3481 ggatgtgctg atcgggaaga cctccccgcc gaggttcctc gaggagccta cgagcgagct
     3541 catcgccgtc gaaaaaaggc gcgacacctc ggtcacgatg cggagcaacg agcacggcat
     3601 cgtggacacc gtcatcatca ccgagggcga gaactcctcg cgcctcgtga aggtccggac
     3661 ccgggacctc cgggttccag aggtgggcga caagttcgct tcccgccacg ggcagaaggg
     3721 tgtcatcggg ctcatccagt cttcggcgga catgccgttc accgagtcgg gcatgacgcc
     3781 cgacctgatc atcaaccccc atgcaatccc gagccgtatg accatcgggc acatgctcga
     3841 gatgctgggc ggcaaggtcg gctccctcga gggccgccgg atcaatgcga cagccttccg
     3901 cggcgagcgc gagacggacc tccggggttc gctcaaggaa ctcgggttcg cgcacaatgg
     3961 ccgcgaagtg atgtacgacg gtatcaccgg tcggcagttt gcagccgaca tctacatcgg
     4021 tgtcatctac taccagaagc tctaccacat ggtatcgagc aagatgcatg cgcggtcgcg
     4081 cggtcctgtg caggtgctca cccgccagcc gaccgaagga cgtgcccgtg agggtggtct
     4141 ccggttcggt gagatggagc gggatgtgat gatcggtcac ggtgcggcca tggccttaaa
     4201 ggagcggctg cttgacgagt cggataaggt gacccagtgg gtctgtgcga agtgcggcat
     4261 ggtggcgatg cttgaccgga agaggaaagt cacgcggtgc cttgcctgcg gcggcgagac
     4321 cgacatctac cccgtcgaga tgagctatgc cttcaagctc ctcctcgacg agatgaagag
     4381 tatgggcatt gctccccgcc tgagactcga tgatatggta taagagaggg ggcagtttca
     4441 aactatgacc agtccaaaac gtgttggaag gatcgagttc gggctcttct ccccgaagga
     4501 gatccgcaaa atgagcgtcc ggaagatcat ctgggcggac acctatgacg acgatgggtt
     4561 tccctacccg cagggtctga tggacctgaa cctgggtgtc atcgatccgg gcctccggtg
     4621 caagacctgc gaccagaagg cagccgattg tccgggtcac ttcgggcaca tcgaactcgc
     4681 gaagcccgtc atccacgtcg ggtacacccg gctgatccgg aaactcctgc gggtctcctg
     4741 ccggtcgtgt tcccggctgc tgatgacggc ggaagaggtc gagaagatta tcggccccga
     4801 agacagcgag cttgcagggg aactcgtctc cgagaaggat atcaagaaag agcgggtctg
     4861 cccccactgt ggagagcagc agctcaagat caacttcgag aagcccacca cgttctccga
     4921 agtctttgtg gaagacggga gaaaggtgga gcacaagctg acccccgccg acatccgggc
     4981 gcgcctcgag aagatcccgg acgacgacct gcgggctctc ggggtcaacc ccgacgtcgc
     5041 gcggccggag tggacaatcc tcaccgtcct cccggtcccg ccggtcacga tgcgcccctc
     5101 gatcatcctc gagaacgggc agcggtccga ggacgacctg acccacaaac tcgtggacat
     5161 catcaggatc aaccagcggt tcaaggagaa ccaggacgcc ggtgcacccc agttgatcat
     5221 cgaggacctc tgggaactcc tgcagtacca tgtcacaacc tacctcgaca acgaggtggc
     5281 agggtgtccg cccgcccggc accggagcgg tcggccccta aagaccctct cgcagcgtct
     5341 caaagggaag gaaggacgtt tccgcggatc gctctccggt aagcgtgtga acttctcggc
     5401 ccgtacggtg atctctcccg acccgaacct ctccatcggc gaggtcggga tcccgcttgc
     5461 catcacaaac gagatgacgc ttcccatccg ggtgaccccg ttcaacatcg atgaactgag
     5521 gcagtacgtc ttaaacggcc ccgagcgccc caccatcaag tcaccctgtg gtgcgaacta
     5581 cgctattcgg ccggacaaca ggaggatgcg cctgtcggac gcaaacctgg agacggtcgc
     5641 ggagatgctc gagccgggct ggaccgtgga gcggcagtta aaaggcgggg atattgtcct
     5701 cttcaaccgg cagccctcgc tgcaccggat gagcatcatg gcccaccggg tcgtcgtgat
     5761 ggatgggaag accttccggc tgaacccggc cgtctgccca ccctacaacg ccgacttcga
     5821 cggtgacgag atgaacctgc atatcccgca gaccgaggag gcacgggccg aggccgagat
     5881 cctggtctcg gtgcaggcga acatcctctc gccgaggacc ggtggtccga tcatcggagg
     5941 tatccacgac cacatatcgg gcatcttcct cctgacgcac acggtccggc acttcagcaa
     6001 agaggagacg ctctacctca cccagcacct gcccatcgag cacctgccca agcctgacaa
     6061 actggatgat gacgggaagc ccctctggac caacaagcag gtcttctcgc tcgtcctccc
     6121 cgacgactta aacatgctct tccaggcctc gtcgtgccag aactgtgaga cctgcaagcg
     6181 ggagatgtgc gagaacgatg cgttcgtccg ggtcgtcaac gggaacctga tcaccggcac
     6241 catcgacaag aaggggattg gcgcgttcga cggccagatc ctgcaccgga tcatcaggca
     6301 gcacggcatg aagcgggcag cccggttcat cgatgacgtg accaaactct cgatccgggg
     6361 tatcatgctc gaagggttct cgttcggtat cgacgacgaa gacctgaccc ggaccgagta
     6421 tggccagatt gacgaggtgc tcaacggtgc cctcagtgac gtcgagcgcc ggatcaagat
     6481 ctataacgag gggcaactcg agccgatgcc cgggcggacg cttgaggaga cgctcgagat
     6541 gcagatcatg caggtgctcg gcaaggcccg tgaccgcacc ggtgagatag ccgggcggca
     6601 tctcgggatg gacaacagcg ccgtggtgat ggcggtctcc ggcgcccgtg ggtcgatgct
     6661 gaacctgacg cagatggccg gatgtctcgg gcagcagtct gttcgtggcg agcggatcat
     6721 gcgtggctac gaagaccgga ccctgccgca cttccggcgg aacgagcgcg gtgcaaaagc
     6781 acatgggttc atcaccaaca gttacaagag cggcctctcc ccgaccgagt tcttcttcca
     6841 tgctatcggt gggcgtgaag gtcttgtgga cactgcggtc cggacgtcgc agagcggtta
     6901 cctccagcgg cggatgatca acgcgttgca ggacctcaag gtggcatacg acggcactgt
     6961 ccggacgaca ggcggccgga tcatccagtt ctgctacggc gaggatggaa ccgaccccgg
     7021 caagagcagt tacggctccc cggtggacgt gaagggcatc attgagagcg tcttaaagca
     7081 ggaggtcaaa tgaacagcga tatagagcag cagatcgaag ccctcgatct gccataccgg
     7141 acccgggaag acctcaaagc gagtctcgcg gagcgggacg tcactgacga agagttcggc
     7201 cggatccttg agatggtctt ctccgagtac aggaagagcc ggatcgagcc ctgcgaggcg
     7261 gtcggtgtcg tggcggcgca gtcgatcggc gagcccggca cccagatgac gatgcgaacc
     7321 ttccactacg cgggtgtggc cgagatcaac gttaccctcg gtctcccccg tctcattgag
     7381 atcatggacg cccgacgtga gccgagcacc cctacgatgg cggtcttcct gctcaacgac
     7441 tgggcgttca accgtgaccg cgcccgtgag gtgagctggc agatcgaggc cgcgccgctc
     7501 cacgagttcg gggatatcac catcgacatg gagaacatgc aggttctcgt catgttaaac
     7561 aaggctgtct gcgaccggcg aaagatcagt gtgggtgaga tccttgaggc agccccgagg
     7621 aagatccggg aaaaacggca cttccgtgac tttgacgttg aaggggacac gaaaaaggtt
     7681 tcgatcacat tcacgccgaa gaaccaggag agttatcaga acctcttcca gctcgcagag
     7741 cacgtcaggc acgtcatcgt tcagggcctc gacgacatcg agcgggttgt cgtcagaaag
     7801 gaaggcggag agtatatcct ttatactgag ggctccaacc taaaagatgt cttcgaagtc
     7861 gaaggagtcg atacctcccg cacccggagc aacaacatca gcgagatcgc cgatgtgctc
     7921 ggtatcgagg caggccggaa cgcgatcatc catgaggcct taagtaccct gaacgaacag
     7981 ggtatcggcg tcgatgtccg tcatatcatg ctcgtcgctg atatgatgtg catggacggc
     8041 gaggtcaagc agatcggccg gcacggtatt gccggtgaga aggagagcgt cctttcccgg
     8101 gctgcattcg aagtaacggt caaccacctg ctggatgcgg caatcgcgaa cgaagtggac
     8161 gaattgaacg gcgtcaccga gaacgtgatc gtgggccagc ctatccagct cggcaccggt
     8221 gatgtgaaac tcatcgcaaa acccgtaaac ctgaaaatct aggagaatca tctatggact
     8281 ttaatgcttc actacgtaaa gccgtgaaga ctggtacagt gttcctgggt cagaacaaga
     8341 cccgggaatg catcgaggaa ggcaaggcaa aacttgtcgt agtggccaag aacagccctg
     8401 aatctgtcaa aaatctcgtg aaagagatcg atacccctgt ctacgtcttc gaaggctcga
     8461 gtgtccagct cgggaaagcc tgcggtatgc cgtacgttgt cagcgcgctc gccgtggtcg
     8521 agccaggtga atccgagatc ttgagtgctg cgagagtgta gacaatgcca caggtcacgc
     8581 tgactgagga gtgcatgcgc ctcatctccc agtttgagag tcttaccggc gcaggcagcc
     8641 gcgattgcat cgttgataac cgcaacgagc ggatcatctt cgtgatcaat cccggcgaca
     8701 tgggacttgc catcggcaag agcgggtcga gtatcaagaa ggcatctgac gtgatgggca
     8761 aaaggatcga ggtcgtggaa tactccgcag acccggttca gttcctccgg aactgcttcc
     8821 ttcccgccca ggtcaccgat atcgatttcg acaccaatga ggaggatcag caggtcgctc
     8881 tcattgaggt ccgggacgag gaccggggtc ttgccatcgg caaagcgggg aagaacatct
     8941 tcaaggcaaa ggttcttgca cagcggcagc atgacatcgc cgatgtccag ctgatgcaga
     9001 acgagcttgc ctgaacgttc ccccttcttt tttgtcgtag ttttcctgga accctcctgg
     9061 ttgagaggtc gcgtgctata ccccgagctc cggaaaagtt ttatcgggcc tgatccgtga
     9121 gggaggtttc gagcgggctc ctatccagaa attatttata ttggtgtgcg cttcatgaga
     9181 ctctcatgaa cagaggatcc gcctggatag tgaagcctgc cctacctgca gtagaaccgg
     9241 cgagcagggg gcatggagct cccggacgcc tgagggtgat gcagtttggg cgtctgctcc
     9301 tcgcatccgt ggttctggtg ctctgtatcg gcatggtcgc ccccctcgcg tccgcgctcc
     9361 cggacccggg tgagaacggc atcggggatt ctgtgggtgg ggaggcaggc gatgagaaac
     9421 cagccattga gaagctgccg attacccctg tcgtactctc ggacccggtg acggtcacgc
     9481 ctgcagagac cccggcacct gaagaaacgg acgaaaaggc cgtaacggag agggagcccg
     9541 tctccctgat tgccggggag accacatctc cacccaaaga ggtgtcggcg acaacggaac
     9601 cgatttccac ggcctccccg gagcctactc tggcggcgac tgtatccgtt gacaactccg
     9661 acgacgagga gccggaggag accccggggg tgatacccac cctggagcgg tatgctcccc
     9721 cgcggccgag cgcgacgatg gcgaaagcac actattctca cgaccggccg ctggtccaga
     9781 gcggccacca gatcgtcagt cccgcatcgc cgtccacttc cggggtgaat gagaccgcgg
     9841 caacggtcgg tatcgtcgag ccacgcgacg gcctgttcgt caggggtgtc ggggtgctcc
     9901 ttgcccggac tcccgaagac gtcgccctca cccggagcgg ggtggagatc gcgaacctcg
     9961 actggctgct cgacccggcc atgcttctgg gcggccggca cccgcgggca gtgtttgagg
    10021 gggagacgtt caccgtcacc gtgacggtcg aggcgaggaa cctgacggtc acgggggagg
    10081 acccggccta tgtcgtcctg gtgccgccac cgggcgagac cggggtctac gagatcacgc
    10141 ggacccgcga gccccggagc ctccgggacg gagaacgcgg cgtctgggag ttcctggtca
    10201 ccacccgcac cgggaggctg acacttgcga acctcaccct ccccgaggag cggctggtca
    10261 ccaacctgac gtcgaacccg gatctctttg ccttccgggc atacgctctt gccggcaccc
    10321 aggagatccc ctcgattggt gtctccgacc cgatactcgc catccggccc gatgggagcg
    10381 tggacatagc agaggcgtct tcccttcccg aactctacac gtttgcaggt atccccgact
    10441 cgacgctccg cccgtcggag acgatggccg gggcgtgggc ccggggcatc ggccacgaca
    10501 caatcgtcgc cggggcggtc gggcaccttg aggtcttaga gaatcttggg aagggggagg
    10561 taggggccct ctacgccaca gaagagaagg tcggggcggg cgaggtggct gcatcgtcgc
    10621 catcgctgtt cgaccagatc atggagttct taagcgggct gtttggtgct tgagcaccga
    10681 agaacgacgt gccttcagga gcggagttcc taccggtgct cgagctccgc tctttcagag
    10741 cgtcgcttcc ctgaactgtt tccagagata cgcgagcccg aagcaccccg agagcatcat
    10801 atcgccgaag ggtgccgcct ggtaggcctc cggcagcaac cggagccggt ttggcccggt
    10861 gacaaccacg gcaccggtgg cgagggattt tctgaccgcg gccccatggc cgccgtcatc
    10921 gaagacctct tcagaggtca gtgtgccgtc ggcgagccgc cggatgtagc actgcagttt
    10981 tgcgggatcg agcgcgccgg tgtggtgctc gaagagcccg taaatctccc gccctttcag
    11041 ggtgaagcag agggtgtgcc cgtttccggc attgacgagg gttacccccg tcgcagccag
    11101 gtgccgaacc acagggtcgc agagcgctcc gataagggct accggcccgg tgtcggtgac
    11161 gagcgcgccc ggcgcctggt gccggaccgc ctgcatccgg gtcatatcgg cgagcggtgg
    11221 gtcggtagcg agcgagagga ggtcccagtc accggcatcc agccgctccc gcatcagttc
    11281 gaaacggtgg atgcggttgc tgcggtgtgg tgagtagccg tggtcctgga ccgcgatcgc
    11341 gacgttctcc gggtaaccga cgccgaagag ggagagcgcc ctcctgagtt ccggttccat
    11401 gtaatcggtg gtatggatgg tcactgcgtc tcccggaggg gcggcccgga tctcaacgcc
    11461 aagcgcccgc accctttcta agtcgtcgtg gattgtcccg gcggcctccg gcgtggcgta
    11521 gacgggaagg ccgcgtgaga ggtgctcccg tatcgcgccg ctgttcgcgc cgccccccat
    11581 caaaaacccg tccaggaaga ccggacaccc ggcccgggtt gcctgccgga tctttgccgc
    11641 caccaccacg ttcggtgagg ggaggaccag ttttatgctg ttctcgatcg atcggccggg
    11701 ctcgtagacc aggatatcct gggtgcccct gccgacatcg atggcgagga gtggtgtcgt
    11761 cagatcgatc atacgtggcc tcaagcaccg ccgcagcccc atggcaggcg gttctatcta
    11821 aatctcctcg cgaggcgtaa aaagagtatg gtcgcggtca ggcatccgcc cggtctgccg
    11881 cgggggacgt ccctgccgtc gctcctcgat ccggatgcac cggctttagc tcccggtact
    11941 ccttcaagga gaccgtcagg tcgcgggtga aggcgaagta gccgatctgg gtcgtccggc
    12001 agaggttcat cgccatctcc atcagccccc tgggatcggg gcgcgcctcc ccgagccata
    12061 cgtggaagat gttgccgccg tccacgatgg ggaagaagat atgctcgatc tcgatccgtt
    12121 tcgtgagcga gaccggggcc gccggggtga cgtgcgtccc gttggtgtag tagatgggga
    12181 gatcgagcgt cgtgccgagc atatcgagcg ctttgtcggc gtnngtgcgg gcgagggcga
    12241 tcgtcatgtt gtgcttcttc gagagttcgc gggaatacat ctcgagttcg gtcatggccc
    12301 ggacggcgag acggaacgcc tcctttgact cgtggagctg gtggccggtg aagtgctgca
    12361 ccatctcgtt gacgccgacc acgccgatgg tgtagacgag cccttcgagg tccacggcaa
    12421 ccgcaccgcg ttcaccggtg ttcgggtcct ttgggcgctg catcgcaaac ggcatccgcc
    12481 cgttcgcacg gatcagggac atccaccgcc gcttgatccg gtagaggtcg acggcgatgt
    12541 ccatgagcga cttcaactcg gcgaagagcc gttcctggtc gccttctgcc ttataagctg
    12601 cccgcgggca gttgatggac atcacctgcc aggaacccat tgagaagtgt ttcccctccc
    12661 ggaagtagag tttgtcctcg aattcgtcgt cctcgtcggc gagggaggaa aattggtacg
    12721 cgcagcagtt tccggtcacg atgccgagcg cgtttgcgaa ggtgtgggtg ccggtgacat
    12781 cgacgaggtc gtagacgtag tcatcataag gcagcctttc aatagaccgg atgcggaccg
    12841 ggtgaagatc cccgttgatg agccgctcaa cgtctccagt ctgtgcttcc ggccgtataa
    12901 gggatacctg cgcgctgtgg tggcctgatg aggcatactg gctggcatat acgagtccgg
    12961 cgtagtgaag cccgcttgtc ccgatcggca ggcgggtgaa gatccctcct gtctgccgcg
    13021 acgatccggt gaccgccgtc gcgccgtatc ccgtcttctg agagaaccgc tggatctgat
    13081 cctgggccgt gatcgtacag gtataggctg tgagagcgta cggcttgcct gtctgggggt
    13141 gtgcgacggt tgtttcctgt tccatgtagt tcatctggac cccgatacgc tgcgcgagcc
    13201 agaccagttt ctgaactgcg ttccgcgaca ccagttttat ccggatgcct cttgcgtgat
    13261 ggacgtacga accctggtgc ccggtccgga tgctgatgtt tccgtcgcca cggaacgcgg
    13321 catcgaggta tgtccccacc agatccctgt cgacgtcgaa gagcagatcc ggtacgttct
    13381 tctcggcagc acattttcca cagccgatag cgtcgaggag gagatagagg agtttgctgt
    13441 tgacggtcac gttgacggcc gtggcctcct cggagatcag cggctcgtac ccgaggaagc
    13501 ccttgatgca cgtcgcggca tcccgaatga gaccgtcttc gtgctttccg aacgagagtc
    13561 ttaccgtgtg ccgccgtccg ctggtatcgc tgcatccctc tgcaagatag tacccgagga
    13621 acctgatgag ctcctttgtt atggggagaa tccggggcag gccacggttc ttcgcgttcc
    13681 gtatgttcac ttccccgtcc cggagcacga tctcatcgcc atgtccggcg gcgcaaagcg
    13741 tttctgcgac gtcgagttcc cggaccgagt tgttgccgaa ctcggcggtt ttgaccacgg
    13801 ggaggtagtc tccggtacga agcgcctgtg ccgatgtctt cgtgaacctg cctgcattca
    13861 gggcaaagac cgggtgatcc ggcgtgacgg tgatccggcg gcccgactcg agagtgatcc
    13921 tgagcatcac ccctgaatat ttctgccgca tgaccccgct gaagggacgc agggctgccg
    13981 tcatatcggc gatatcgaca ctcggggtat ttgcatcgag gcgggcaaac tctgtgccgt
    14041 acggatcggt gtgcctatct tctccagacg tatcttcaaa gaggtctttg atctccctga
    14101 tccgggttct gccgtcagcg tccactatgg gaatgagttc atcccctgcc aggcactggt
    14161 agcacgagat cccctccccg gcgccccggt agccggggat ctggttgtcg tagtagggtg
    14221 tgccgtactt ggacgcgagt tcgaaggtca tcaggtagag gtcctggtag gtaggaagat
    14281 ccgggtgctc gcgattgaac tcctcgttct cggcgaggaa atcgggctcg atgctgatct
    14341 cgggtttcgg gaaggagaag ggcttgcccc
//
LOCUS       DAJB01000041            3001 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_45065, whole genome shotgun sequence.
ACCESSION   DAJB01000041 DAJB01000000
VERSION     DAJB01000041.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_45065"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          609 a          894 c          941 g          557 t
ORIGIN      
        1 ctcctcttcg gcgggacgat ctactactat atgttccttg agaagtacgg gtgcaccgat
       61 cttgagagcg ctcttgaccg gcttatcctc gacgacctcg cacccctcag gcgggagatg
      121 agcgatgtcg tgatcgagga gttcggtcgt gagcatgcga cgtaccacga gatcctcacc
      181 gccatcgccg aggggaagca ggcgcagaag gagatcgccg acttcgtccg ccttgccccg
      241 acgtcactgc ctccatatct gcgtgatctc gtcgacctcc tcgggatcat cgagtaccgg
      301 actccggtca cggagcgggg aaagcggtcg aagatgggga ggtacgtctt ctccgacaac
      361 ttcttccggt tctatgcacg ctacatctcc aggaacatga gcctgtacca gagcggacgc
      421 tttgctcttt taaaggaccg gattctccgg gagtggaaag ggttctcggg ctgggcattt
      481 gaggagatga tgcgaagcct catcgcccgg gatctcgagg agcgatacga gcatatcggg
      541 ccgtggtgga accgccgtgg agatgagatc gatctcgtcg ccctttcacc gcaaggaagc
      601 cttgcagtcg agatcaagaa ccgcaatctg ttgcgttccg aggcgtacga cctcctctcg
      661 gctcttgtga agaagatccc ttgcgtaaaa ggcctttcgc agccggtagc cgtcggcgtt
      721 gccgcccgca ctgttgaagg gaaggagatg ctcataaagg agggggtcta cgtcagggat
      781 cttgcagatc tcggcttatg aatgaatgac tgacaggtgt tttgtcgatt cggcctgctg
      841 gatcgcgctc ctgaacagac acgataacct ccacgagacc gccgaaacta tctacaaaga
      901 ccgaatggaa tcaggatccg gtttcgtcac cacctcctcc gtgctcatcg agacatccaa
      961 cgcactctgc catcccgcat tcaaaccggc tgttatcgcc tttcgtcgcc gccttgaagc
     1021 gtcgtcccgg gtggagactg tctttgtcga tcccgaccta tggtcgaaag gatggagcat
     1081 ctacgagccc cggctggata aagcctggag tcttactgat tgtatctccc ttgcggtcag
     1141 gagcaggggc tctccgatgc cctgaccggc gaccggcatg ttgtccagac cggctgccgg
     1201 accctgctcg ccgaagactg agaatgcccg gggggagaac tccggcgcac gtataccttc
     1261 agagccagtc acgttgtcga cgagctcgcg ttctaggtcg cggtcattct ccccctgcag
     1321 gagtacgagc aacgcaagga gagaccttgt gccgcgaaga gccggccgta gagttcagcg
     1381 agttgaagaa gcggtacgag cgggatacgc gatccgggtc aaggcaagcg tcgagagaga
     1441 gatcgcggca ttgccgcgca ttcttccagg gtatagaggc tctatccggg acgcccgtcc
     1501 cctttggcgt gcgcaggtta cgtggggcag agcacctcta ccggatccgc gtgggagact
     1561 accggatcgt atacgccgtg gatcacgcag tgcaggaggt tataattctc tatgtccggg
     1621 ccgcagtgca taccgtgggt tgtgaccccg ggcaagctgc cccggtcctc ccgcgtcacc
     1681 gggagaagag cacggtcccc gtctccgctg cccggcgggc gaggggattg ccgagctcgc
     1741 tcaccgtgta ggggaagagg tcgacgccga tcccgatccc ggagaagtac gtgaggtagg
     1801 cggagacccg atcgaggagg gggcgttcgt cgcgtgcgag gacgatcatg atatcgagat
     1861 cgctcccggg aacggcccgc ccctcggcga acgacccgaa gagaacgaca cggaggacgt
     1921 tctggtcgtc tgccagcctc tccaccgccg ctcgtaaccg ttcccggaca aggtctgcat
     1981 caagccagaa gaccgtcaca gaattgaatg attctttccg caccactgac tgcatcgtct
     2041 gcatcctccc gggtgaaata gtctgccggc ttcccccggg aaaacccgtc cgggtagcgg
     2101 gctggaatat acgagcggtc gagaccgcgt gcgatccgga agaggtcaga ggggatctcc
     2161 cggccggctg cccggagacc atcgaggaga tcgagaacgg agtggcccca cgcaaccgcc
     2221 ccatgcctct ggtagaccgc ctttatcgcc ttctcggcag cctgctgaga gataaagcag
     2281 gcccattcga agaacccggt atcccgggcc tgcccggcca tggcgagatc ccgccgcgcc
     2341 tgctccatcc agtccgcact ccgctcgggc ataccgtaag agagtataga ggcgcagtta
     2401 tatgaatgta gggttgcgtg ccgggccgag cggttcggac ccaccccgtg agaggggcga
     2461 tctccaggtc gccggcggtg acggccgggg gatctgccct gtcgcttccc tgagcgcgac
     2521 ggcatcgcgg atatcctcac agcaggagaa ggaaatccca cgtatagacc atctcgccgg
     2581 cgacagcatc cgtggtttca aagtctcggc gatgacgggg tccggccccc gccccgggag
     2641 gagaaccgcg gcgcacttat atcttcggag cgacaacgca tggtaaccgt cgggcagaga
     2701 ccatggatgc gaatatgcag gaagcggagc ggtggctgcg gcagggggag agagatctcg
     2761 tcagcgcaag gaacagctgc cgatcgggcg attttgaatg ggcctgcttt caggcgcagc
     2821 agagcgcgga aaaagctctg aaagccctgc tgtatgcccg cggattgcgg aagatcctga
     2881 cgcactcggt ctacgaactg acgagagagg tcggtgcgtt cgagccctcg ttcctggagc
     2941 tgaagagcgg agcgaaggca ctcgacagcg cctacatcat gacccgctac ccggacagca
     3001 t
//
LOCUS       DAJB01000042            2871 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_83325, whole genome shotgun sequence.
ACCESSION   DAJB01000042 DAJB01000000
VERSION     DAJB01000042.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_83325"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          525 a          996 c          887 g          463 t
ORIGIN      
        1 ggaccgcggt cgagacctgg gccgatgccg tcacgtcgct cgactgaagc ggggacaacc
       61 tccctcgctc tttttccccc gggtgacggg cagagccttt gtagcgggga tatctatacg
      121 taagagaaca acgcatatcc cgatcaggaa tggataccgt cacccgcgag gccggcggag
      181 agtgcgcacg gatcatcggc attctccgga ggaagaaggc gtacctggaa gagacctatc
      241 acgtcgggtc catcggcatc ttcggctcgt gccggcgcgg cgaggagcgc gaggaaagcg
      301 acgtggacat tctggtcgag ttctcagaag tcccggggat cttcgggttt ctcaggctcg
      361 agcgctacct ctccgagatc ctcggcagac cggtggacct ggtcgaaaag agtgcgctca
      421 aaccccgtat cggccgccgt atcctgaatg aggttatcta cgtatgacgg ctccgcggaa
      481 caccctcgac tacctggacg atatccttga cgccgtcgat aagatcgcga tctttacccg
      541 gggcatgtcg tatgaggagt tctccaggga cgataagacc gtgtacgcgg tcacgcgagc
      601 gctggaagtg atcggtgagg cgacgaagtg tattccccgc cccgtcaggg agagttatcc
      661 ctctcttccc tgggccgaga tggccggtat gcgcgacaaa ctgatccatg cctactttgg
      721 gatcaacaaa gcgatcatct ggaggacgat ccgggatgat atctcttccc tcaggtccgc
      781 gatgcaggcc ctccgggatg acctggccgc ggacggatcc acgagcagcg agcgccggta
      841 agcacgaacg gtgacaaggg ggtcccggcc tgcaaccggt tagtgcggcg ctcccggtgt
      901 acaccctgtt cttcacgaga ccggtaagcg ggctcatcac tttcaacctg cgcacacctt
      961 gtctttaaca ataccctctt ctccagactc ttctatgaca cgcctccccc ttctccgcag
     1021 cgaccagacc ctcttccgcc accctgacgc cttcgagccc gcgttcgtcc ccgaccacct
     1081 ccaccaccgc gacgcccaga cccaggaact cgcccgggca tcctccggcc cgccacccgg
     1141 ggcgggagcg ctctctccgc cgtcctccgc ggcccccccg ggaccgggaa gaccaccacc
     1201 gtccgccgga tctttgccgc ggtcgccgaa gagacccggc aggtgatccc ggtctacgtc
     1261 aactgccgcc acgaccacac cccgctcgcc gtctaccgga gcatcttcaa acaggtctgc
     1321 ggctacgccc cgccgtcggc cgggcgctac ctcgacgaga tcaagcaggg gatcgcgacc
     1381 cggctccggg accgcaacgc cgcgctcgtc gtctgcctcg acgacgcaga cgaactcatc
     1441 ccggccggga cctacaacac cctcctctac cagatcctcc ggctctacga gaagtgggat
     1501 gtccggaagg ccggggtctt cgcggtcacg agcgacctcg ccctgaacct ctacgcggag
     1561 gccgacgcga gcgtccggtc ggtcttccac ccgaccgagg tcaccttcct gccctactcg
     1621 aggaccgagg tccgcgagat cctcgccgac cgggtccggc agggcctcta tccccgggtg
     1681 ctgccgaagc cgctcctcga ccggatcgcc gctatcgccg ccgatgagca ggacgtccgg
     1741 gtcgggatcg accttgtccg gcaagcgtgc ctccgggcgg agaaggacgg ccgccggcgc
     1801 gtcacccacg ccgacgtcac cgccgcagcc cgggcggtca cggcgccggc cctccggacc
     1861 cgtgctgccg ggctctcgga ggccgagcgg gcactcgcct gctggatcgc ggagcggtcc
     1921 cttgcaggcg ccgataccgc ttcgggggcg gtctttgaag cggcacagga gtatctcccg
     1981 gtcgggaaga cagcctacca cgagcacctc aacgccctcg caaaggccgg gatcgtcgat
     2041 cttgtgcccg gggcgggccg ggggagggag gtccggctcc gctacgatgc cggggacgtg
     2101 gccgccgcct gcaaagcccc ggaccagacc tgcaaaatcc cgaactgagc atgagatccc
     2161 ataacccccg gtccagcccc ggattggaga tcctcatcag atgctcattt tccaaatttt
     2221 tccaataaag caaggcttat atggggcctg ttgctttttt tgggatggtt ggctcactct
     2281 tttacgcaac agctcccgat gatggtgtat gcgccggaaa gaatcccggt gcagggagtt
     2341 atcagaaatg caacacgaca ccagacccga ggagaacaaa accccgggag gaaccgaaca
     2401 gaaaaccaga ggcggcagcc cggatccaaa gtgccaccac gcgctccgcc aggccgccac
     2461 tggtacatct gccccggaag agataaacgt ctcggcggtc ccgctcttcc tcgtcccgcg
     2521 agcggtcgcc gccgagatcc ggcgctacgg ccgggcggtc cgcgagatca ccgtccgccg
     2581 gacccgcaac caccagtaca cgatcaccgt cgggcggggc cggccatgat ggagcccggc
     2641 accctcgcct gtaccacggc gggcgggctc atcgtccacg tcgaggcgga gcgctgccgg
     2701 atcgcgcccg atgacgtgag aagcctgatc ttctccggcc gcccggcgcc ggtcacatgc
     2761 gagcgggtgc ggcgcaggga tggcatcgtc acgggcgagg tgacgatcga gggccatgcg
     2821 gcggtgaacc cggccggaag agcggtggtg gtccacaccc ggacgggatc g
//
LOCUS       DAJB01000043            7729 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_69311, whole genome shotgun sequence.
ACCESSION   DAJB01000043 DAJB01000000
VERSION     DAJB01000043.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_69311"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    124..217
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1400 a         2276 c         2421 g         1538 t
ORIGIN      
        1 ccaccgccac cgtcacgaag agcaccagca tccccacttt caggaaatcg ataaaggaga
       61 tgccgatccc ctcgtgctcg gcgataccga taacgacaac gtttgccgac gccccgatgg
      121 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag gagacgttct cgaccatgac
      241 cgaggcgatg ttcgagatga cgccggtctc gacgagggcg ccgacgatga cgaagagccc
      301 gccgaagaag aagagggccg gccactcgat cttctcaaag atctcctccg gcgactgcct
      361 gctccagaag agaaggatcg ccgccccgat gagggccacc tcggcaggct caagccccat
      421 cgccgggtcg acgaacggca ggaagatgtg caggatctct ccgatccggt cgtggacgaa
      481 gaagagcagg actacgaacc caataaccgc caccgacttg ttgaagagcg accggtcgac
      541 gatcgccgcc cgctcgtcga ggccgttgag cgtccggacc aactcctgcc gctcgtcggg
      601 gctcaccttc atggaccggc cgtagatgag atacatcatc agaaggagga tcgccatatc
      661 gatgaccatg atggggccaa ggtggatcag gaactcgttg aaggtcagtc ctgcagacga
      721 tgcgatcatg atgttcgggg ggtcgccgat gagtgtcgcg gccccaccga tgttggaggc
      781 aaagatctcc gtgaccagga acgggatggg gttgatcttc atcaccttcg cgatgtagag
      841 gagcatcggg gtgaggagaa ggaccgtggt gacgttatcg aggaacgcgc tcacaaccgc
      901 agtcacgagc gcaaagagga tgaggacccg gatcgggctg ccttttgcca gtttcgccgt
      961 ttgtatagcg atatattcga agaggccgct gccgcgagcg gtattgacga tgatcatcat
     1021 cccgaggagc aggaagatcg tcccgaagtc gacgtgctcg aggagcgcct cccaggggac
     1081 gatcccgatg aagacgacga tcgctgcccc catcatggcg gcaaccgccc ggtggatccg
     1141 ctcgtctacg atcagggcgt aggtgaagag aaagaccgct actgcgatca gcgcaacagt
     1201 ttccatcgat gtacctgtca gtagatgagc gcaggggtct ccacccgctg gcagatgcgc
     1261 aggacgatcg ggctgatcga gacgtcggtc gactccccgt cggcatagcg tcggcagagc
     1321 gcgaccatgt cgtggttctt tgccatctgg acgacatcgt cgcccttatg cccgacgaac
     1381 atccgcgtct gggtcgaaaa gccgtgttct tcgagtttcg ccgcggtccg atccagaagt
     1441 ttccggccgt actcttcctt ctttgcccgg aattcctccg tcgatgactg gttgagcgcc
     1501 gcccgctcga ggatctcgat caccccggca tcggtgatgt aggcaagcga aatggtggca
     1561 tcgtaggcgg agaggacgtc gatggcttca tcggtgatat catggacgaa gaaatcgagc
     1621 ggcaccagga tggaccggac ttcggggaca agcagttcct cttcggtcag caggaactca
     1681 ctatactcct tgagaaccga ctcgtagcgc ttcccgacga tgtctttgaa ctttcgctga
     1741 atcaaggatg atgagtatcc catagtaatg cactcctagg tatccagaaa ctctcgctta
     1801 atacttttgc aactttggcc ggctctgcgg gcacctcttc cgggaacgcc gggagatgct
     1861 cccgatagga gatgtcggcc gtcgtcatct ctctttccct ggcgcgagcg cctgcctgca
     1921 gagcgggcag agcatcttcc tcttccggtc cagttcgtcc agcgtccgcg gccggaacat
     1981 aacgcattcg gggttgtcac agtggccgag cccgaggagg tgcccgagtt cgtgcgctcc
     2041 ctcttttgcg gtccggtcga tcaggtcggt gtcgtccggc gtcctgccgt agtaaccgtt
     2101 ccctaagcgg gcagtcgaga cgacggcgac gccgcttgcc ggcctcgcga ggccgaagac
     2161 gaagtcgcat ccgttgacgt agaggtcttt tgaggtgacg agcagcaggg gcccggggat
     2221 ctcgtacctg cgggtgaggg tatcctgcag gcggtccaga atattttgag catcgtgctg
     2281 attgcggttc cggtcgtagc cgtcgatgag aaacggatat tcgacgagcc cggtctctct
     2341 cccgaggatc atcgcaatca tccggatgac cgggagctcg ataccttccg gtgcctgctg
     2401 gtcccaaaga atattgatgc ccattaccta atctctgggt aatgtgtcgg tataaacata
     2461 ttgaacagag tgtgggggag tatatcgccg gtcgttaccg gcaggccgtg gaagtgggca
     2521 tcggcaacaa tcccgttgcc gcacgggtga tcgcggccgc cgggggtctt atgctctgca
     2581 ccgatatccg gcccggcatc cggcacgacg ggcttaccgt ggtgaccgac gatatcttcg
     2641 agccggatct ccggctgtat gcgggcgcgg acctgatcta cgccgtgcgc cccggtgtgg
     2701 agatggttcc gccgctgatg gcacttgccg gccgggtcga cagcgacctc ttagtctatc
     2761 acctggggtg tgagatctac gaggacggcg gcgaggtcgt ggactgcggc cccgtccttc
     2821 tccaccgcta ccaccgccgc cgttcttaga agagtgtcgt ctggccctga gggaggtcct
     2881 gccgcatctc cccggcatca ccgggagggt cgtcgtcttc gggcttcctg cctctgcctg
     2941 atgtcttctc ttttgcgggg gccttcttct ccttcgccgt cttctccttc gccttctcct
     3001 cttttgccac gtctttgacc acctttgcag cccgggcctt gtcgtggatg aagaggttca
     3061 gttcgtcggc gtcgagcccg aactcccgga cgtatccggc cgggtcctgc tcgacgagga
     3121 tgctgatcgc ggtgaagaaa tcttcacgca gggcgtcctc ggggatatgt gtcagatcgc
     3181 tgagcttccg ggtcagccct gccctgaccg ccttctgctt cttcgacgcc cccatcttct
     3241 gccagcgcga cggcggcatg atgcggctgt ggatgccgtg cccgccggcg gcgtcggcga
     3301 cgccgatgag catgactgcg ctcgcgtacc gccagagggt gtaatactgc cgccggtagg
     3361 tccggccgac gtactcgtcg gcccgtgaga ggcaggcgta gccctttgcc cgggaggcga
     3421 ggtcgggcat attgtcaagg ctcccttcaa gccactgttc gatggcatcc ggggtatcct
     3481 cgacctccac cgccatgcgg agcaggtcgg catcccgccg ccccttaaaa acccccccaa
     3541 cgagttcgaa gatagtggac cgctcgtcct ttgcagaggt gtggacgtca ccggcgagaa
     3601 ggtgctcctt cccgattgcc gccgcgtaga gcatgttcac cgcggcccgc atatctccgc
     3661 cggcgcgttc ggcgattgca tcgagggcgg gaggatcgca cgccacgccc tctgcggcac
     3721 agatctggcg gaggcggggg actatcgagc gggcctggag cgcccggaac tgcaccggct
     3781 ccgtggccgc tttgaggtcc cctgggaggg agtagtagtc gtttgcaatc aggatgatcg
     3841 gctgctgcga cgctgcgatg atctccacga tcgccttcgc ccccccccgg tccgcctgcc
     3901 cgtgcaggtt gtcggcctca tcgagcagga tgagtttacg gctcgcaccg gtgagactgg
     3961 ccgtggtgga acctgcgccc gccaccctat cgagggccag tttggtccgc tggtcggagg
     4021 cgttcagctc caccacctcc cagttcatgt cgtttgcaag ggcgtgagcg cttgaggtct
     4081 tgccgatacc gggtttcccg tacaggatca acggcttttt ctgccgcgac cagtcctgcg
     4141 cccactcgta catctgcctg acggcgccgg agttccctac aacgtcctgg agatgctgag
     4201 gcctgtactt ttccacccag tccatatcga tctattcgac gcggtgtatc aaaaatacca
     4261 ccccggcgcg gacgcacaga ggtcccgcgg cacccggcag gggagccccg cttacccggg
     4321 ccggatgcct cacggacccc cttcccgatc ggtgccggac tgctctcccc tgccgggatc
     4381 ggcgtccgga acagagtgca taataccggt gaatcaaata cattatctct gatgaggcag
     4441 aataccataa agaaacgtgg agtgttggtg ttcttgtggt gaaggattgc tccaccgata
     4501 cggtagcaca ggccgtcagg gaatacgagg gcgttacccg gaagaaggcg atcgggaata
     4561 tgatccggtc tctccggatg gagagccccg atgttgtcgc ttcgttcggt gaggatgctg
     4621 cggtcatccg gcataacgga gaggatgcgc tgctgcttgc ggcggacggt atctggagca
     4681 agctgatgga agcggatccg ttctgggcag ggtactgtgc cgtgctcgtg aacgttcacg
     4741 acatcgctgc catgggaggg agacctgttg cgctggtcga tatcctctcc gtcacgaacg
     4801 accggatccg cgacgaggtg acccggggca tggtcaccgc atccgcgcag ttcggcgtcc
     4861 cgatcgtggg cgggcacctg cacccggaga caccctacag cgttgtggat gtggcgatcc
     4921 tcgggacggc caggatggac cagctgatct tttcgaatac ggcagaggag ggtgaccggg
     4981 tgattgcggc gatagacctc gacggccggg tacatccgtc aagctgcttc aactgggact
     5041 cggtcacgat gaagtcggca gaggaggttc gggcgcagat ccgggtgatg gaggatctcg
     5101 gcaaggacca tctggtgacg gccggcaagg atatcagcaa cccgggtgtg atcgggaccc
     5161 tcggtatgct ccttgaggtg agcgagaagg gtgcggtgct cgaccttgag gcgatacccc
     5221 ggccggacct cgccgcgatc gatctcccct tcgagcaatg ggtgcgcatg tatcccggca
     5281 tgggattcat cctgaccgtg aaggaggaga acgtcgagga agtctgtcac cggtttgcag
     5341 acgtcggcat caccgctgcc tccatcggtg aggttaacgg gagccggaaa ctgactatca
     5401 ggcacctggg ccgggagacg caggtctttg acctggacca gaacggcatc atgcggatct
     5461 tcgctgaagg cggggcatgc cggtaacggt cgggcttggt gcggcatctc ctgatgcggg
     5521 gatcctctcg accgtccggg cgatcggccg tgaggttgac cttatccttt tttcctaccc
     5581 ggggactgcc ggttcgtttg gtgatggggt ggccgtcgtt gaggccgagg agccagaaat
     5641 cgcccttatc gaggctcttt atgccggcag gatagatgcc gccgtcaggg gcaccctgcc
     5701 tgcgtcttcc accttaaagg ccctcaaaac agctgcaggt ctcgaccacc tggagcggat
     5761 cgcccttctc gagactgccg acggacatct ctttctcctt gccccggttg gggtcgacga
     5821 gggctggacc gtcgaggaga aggtgcggct cgtcggacac ggccgggata tcgcccggag
     5881 tttcggcctc gccgaggggg tggcgatcct ctcgggagga cggctcgggg atctcgggcg
     5941 gcacccggtc gtcgaccgga cgatggcgga tgccgaactc gttgcccgcc tgaccggtgc
     6001 cgagcacacc gagatcctga tcgaggaggc agccaggcgc tacgggatgg tcgtggctcc
     6061 cgacgggatc tcggggaacc tgatcttccg tacgctcacg ttcctcggag cgggggccgg
     6121 gcacggtgct ccggtggtaa atatcgataa aattttcgtg gatacgtcgc gcgcctcagc
     6181 aaattacacg aatgcagtaa tgctggcgaa atctctggcc gagtccagaa aaacctgaat
     6241 tatggctcta ttgctgaaac agccgggttt ctgacgtttt tcaatcggca cgattctctg
     6301 ccgggtaacg gggtgccgtg tccggatgta accgcgatag ggaataaaaa cgcaaatttc
     6361 cgagataatc ccgaaacgtt taaatattaa ctcagacact tctttttaga ggtggaagta
     6421 aattatggca gatctaccta ttgctgcggt tgtacgtatc gcaaagaaga atggtgctga
     6481 gagagtcggc agcgacgctg ctgccgcact ggttaccaag gctgaggcgt atatcgctga
     6541 gctgaccaag gaagccaaca ggcttgccca gcacgcggga cgcaagacga tcaaggcaga
     6601 agacgtcgat ctcgcggtta agtccgcgta aatcgactgt cctcccttat ttttaccggg
     6661 ggccctccgg gcccccttct ctaatctatt ccggcagcat acatgccgat gcatcctcct
     6721 cccggggtct tcgcggtgac cggatagttc cacggatgtc tttgtggacg gtaccgtgac
     6781 cgtttgctcc agcgatccat accccgtaac tgtcgagtga tggctgcccg gacaactgat
     6841 atttccggca tccggcaggc atccccgtgc cgcagaccgg gagtctgtgg cggcgatggg
     6901 cgccagacgc tcccatcttc gatctcagcc cggttatccc cctgggtggt gtgggggggc
     6961 agctgccgta taacgcaggc tctccgggct gatgtgtcgg ttttttgggc gacggttctc
     7021 tcccctcccg gagaagtcgg ggtatggcgg ccgtaaaccc ggccaggtag aaaaaaggtt
     7081 cccgctctct tccggtcaga gcgtaccttt ggtgctcggc acgtcgccta tcgaggggtc
     7141 gatcgccacg gccgccgcga gtgcccgtgc aaacgccttg aacgccgcct cgcagatatg
     7201 gtggtcgttc ctgccctcgg ccgtgatgtg ggcggtgatc ccggcattgg tgcagaggct
     7261 gtagaagaag tgctcgatga ggtcgccggg tatgccgccc gggcctgccg gcgagaaggc
     7321 actctcgaag acgaggtaac cccgaccgcc cacatcgagc gccacccggg cgagcgcctc
     7381 gtccatcggg acggcggcgt cggcaaaccg ggtaattcct ctcccatcgc cgaccgcttc
     7441 ggcaagggct agcccgagga cgataccgat atcctcgatg gtgtggtgga cgtccactgc
     7501 gatgtcgccg gtcgcccgaa cagtgaggtc tatcctgccg tggcgggcaa acgatgtcag
     7561 catgtggtcg aagaacggta tcccggttcc gatgataccg gccccggtgc cgtcgaggtc
     7621 gagggagagg ctgatatcgg tctctttcgt ggtgcggtgg atctcactct gccgcatgcg
     7681 ccgcctccag gacctctgaa agtcgcacct tccccgcgta gagagccga
//
LOCUS       DAJB01000044           21940 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_71643, whole genome shotgun sequence.
ACCESSION   DAJB01000044 DAJB01000000
VERSION     DAJB01000044.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21940)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21940)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21940
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_71643"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    421..451
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3688 a         7064 c         7170 g         3985 t
ORIGIN      
        1 gatgcagtag ttccgacccg tgtggtgccg ggcaagtttc atcgccgcct caacgctctc
       61 cgccccggag ttggcgtagt agacccggtc gagcccgtcc gggaagaacc cgaccagttc
      121 ttcggcgcac cgtaccggga tctcagagca gaaatccatg aacgccccat gtgagaggga
      181 gttcgcctgc tcttgaacgg cggcgacgac gttcgggtgg ttccagcctg tgttcatcac
      241 ggcgatcccg gccgtgaagt caaggtagcg attgccgtcg acgtcccaga ggttcacgcc
      301 gcttgccttc tgcagcacga gggggtactc ccgcatgacc gacggggaga tgaccaccgc
      361 gtcccggcgg agcaccccgc gggctttctc gcccgggggg ttcgctgtgc atcaggggct
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcaaccctc accgcctgtt ttgagaggct
      481 cgaagcgaca tcgtcccgga acgagatgac cacgcttctc gcccggctgc tcgccgcggc
      541 atcgccggac gagatcggga ccgtctgcta ctttgtgctc ggggagatcg gtcccggcta
      601 cagcgacgtg aacctgggca tcggggaccg gaccacggcg gcggcgatcg cccttgcggc
      661 cggcaccgac cctgccccgg tcgaagcggc ggcgcgggaa ctcggcgact acggcgacgc
      721 cgcattccgg ctgatcgcgg cccccgccac ggggccgctc ccggtcgccg acgtccaccg
      781 cggcctcgtg gagatcgccg gggcatccgg cccgggctcg gtcgaagcga agaagaacgc
      841 cctcgcggct cttcttatcg ccgccacgcc cccggagcgg cgctacctct cgcggcttgc
      901 cgcgggggag atgcggctcg gcgtcgggga gatgaccctc ctcgatgcgc tggcggaggc
      961 gttcctgggg tcgaaggcag agcgnntcga ggagggcctc cccggggtgc aggcgatcgc
     1021 cgtcaccctc caccgcccca ttcggccgat gctcacccag cgggtcgccc ggatatcgga
     1081 gatcctcgag cggatcggct ctcccgtcat cgccgtcgag gagaagtacg acggcgaacg
     1141 gatccaggcg cacaaggacg gcgacgacgt caccctcttt tcgcggcggc tgaccgacgt
     1201 caccggccag taccccgata tcgttcggca cgtccggcgc tctgtcacgg ccgatacggc
     1261 gatccttgac ggcgaggcgg tcgcgttcga cggcgagacc ggcacctacc ggcccttcca
     1321 gacgctgatg cggcggcgcc ggaagcaccg ggtcagcgaa gtcgccgacg aggtcccggc
     1381 gacctaccgg gtcttcgacc tcctctatgc cgacggcgaa tcatacctct gccggagtta
     1441 ccctgaccgg cgggcgaggc tcgaggagat cctcgtcggg gacgagcaca tttctcccgc
     1501 gggccgcatc gtaacggggg atgccgacgg gatcacggcg ttctatctct cctgcatcga
     1561 gcgggggctt gaagggattg tctgcaagtc gtgtgcagag gactcgttct accgggcggg
     1621 ggcgcgggag tggcagtgga tcaagtggaa gagcgattac gcccccggga tctccgatac
     1681 gctcgatctg gtcgtcgtcg gcgccagcgc cgggcggggg aagcgggccg ggacctacgg
     1741 ctcggtcctc tgtgccgcct ataaccgcga ggaagacctc ttccagacgg tctgcggcat
     1801 gggaacgggg ttctcggatg aggaactctc ggacctcccc cgccggctcg ccggtgcgag
     1861 ggtggaccgg cagccggcgc gggcgatggt gaacccgcag gcggcgcctg atttctggtt
     1921 cgcgccgcgc tatgtcgtgg aggtgctggg ccttgagatc accgagagcc cggtccacac
     1981 ctgcaactgg gacccggagc ggcggcgcgg cctcgccctc cggttcccga ggttcgtccg
     2041 gtggcgggac gagaagggcc ctgaggaggc gacgacgacc gccgagatcg cggccatctt
     2101 ccgccgccgg cggggggtgt aacggtatgg agcagtactc acggttgacc aacatcgagg
     2161 tcgcggcgat cctgtacgag gtcgccgacc tcctcgagat caagggcgtc cggttcaagc
     2221 cgcaggcata ccggcgggcg gcgcaggcga tcgagaccct gccggaggat gtcggcatca
     2281 ttgcaaagga aggagacctc gatgagatcc ccggggtcgg caggggcatc gccgggaaga
     2341 tccaggagat cgtcgagacc ggcagcctcg cgtatctctc ctccttacgc gaggagctcc
     2401 cggagggcgt gcagcacctg accgggatcg aggggatcgg gccgaagaag gcgatcgccc
     2461 tctcccggga actcggtatc cggaccgtcg acgacctgga ggcggcggcg ttggccggcc
     2521 ggatccgcga cctccctgga ttcggggaga agaccgaagc aaacatcctc gcgagcatca
     2581 aggtgagccg gacggcaaaa gagcgccgtc tcctcggcta catcctgccc gtcgcccggg
     2641 atatcgagcg gcggctcgtg tcgctccctt cggtcgggca ggtgagcctc gccggatcga
     2701 tacgccgcag gaaagagacg atcggggatg tcgacatcct cgcgacctcc tcgcgccccg
     2761 aggaggtgat ggaggtcttt gccaccctcc cgggggtctc gcgggtcctc gtccgcggga
     2821 cgacgaagac ctcggtggtg ctcgcgaccg gcatccaggt cgacctccgg gtggtggagg
     2881 acgtccactt cggggccgcc ctccagtact tcaccgggtc aaaggagcac aacatcgcgc
     2941 tcagaaaact cgccatcgcg aggaactggc ggttgaacga gtacgggctc gtggacctcg
     3001 caagcggccg ggcggtcgcc ggcgaggacg aggcgggggt ctaccgggcc ctcgatctcg
     3061 cctggatcga gccggaactc cgggaggacc gcggcgagat cgaggccgca cgggccggaa
     3121 ccctgcccga cctcgtcggc tacgactcga tccggggcga cctccacgtc cacacccgct
     3181 ggagcgaggg cgcccactcc atcaaggaga tggcggaaac ggcgcaggcg ctcggctacg
     3241 agtacatcgc catctgcgac cacgcagaga ccctccatat cgcccacggc ctctcccccg
     3301 aacgcctggc cgaccaggtg cgcgagatcg agcggatcaa ccgggcggct gaaggcggct
     3361 tcaccattct ctccggcacc gagtgcaaca tcggcatgga cggtgagatc gatcttcccg
     3421 acagcgttct tgccgacctc gacgtggtgg tcgcgagcgt tcactccggg ttcaagcagc
     3481 ctgaggggga gatgacggag cgggtcatca cggcgatgca gagcgagcac gtcgatatca
     3541 tcggccaccc gacgggacgg attcttcttg cgcgggagcc gtaccggatc gatctcgccg
     3601 cggtcttcga tgccgcagca gcgctcggca ccctgatgga gatcaatgcg ttcccgggca
     3661 gactggacct ctccgacgtc aacgcccgct cggcccgcga gcataacgtc cggttctcgc
     3721 tcggttcgga cgcgcacaac cgggagaacc tccggtacat ggagttcggg gtcgcgaccg
     3781 cccggcgggg atggcttacg ccggacgata tcgtcaacac ccgcccgctt ccggagttaa
     3841 cggggttact gaggccttag ggattatccg gttgcccgct cccgtgcggc gctgacgaca
     3901 tccacgagct ggatctcgaa tatcaggggc tgcccggcca gggggtggtt cgcgtccagc
     3961 gtcacggtcg attccgagac gtcgctgacg acgacgacgg ccgccctgcc gtccggctgc
     4021 tggacctgga gttgctggcc cggctcgggg ttgatctcct cggggaactg ccccctgtcc
     4081 accgtaagcg tcatatcctc actgcgtggc ccgtatgcct cttcgggcgg gatcacggcg
     4141 gtcttcttct cgcccggttc catcccgatc acggccttct cgaaccccgg tatgatctgc
     4201 cccctgccga tggtgaactc aatcggtgct tcttcatctg aggtgtcaaa gaccgtgccg
     4261 tcttccagct ttccggtata atgcaccttg acggtatcgc cttcctttgc ctgtgccatg
     4321 ctgcttcacc ggcggggtgg tgatcccggg ttcatattta tgtgtgccgg ccgggcccgg
     4381 gctgccggtg tttggcgcga tctcccggca ttccgggagt ttgcagggga ggggaggcgg
     4441 tccccgggca tgactgcctg caagtggacc gtggggatat cgcctcgcgg ggtggggccg
     4501 acggggagtg cgagcgtagc gagcttgagc accgttaggt gcgagggggt ctccccctcc
     4561 cctgtctcca tctcacaagt agatgtgtaa cccccacctc gcccggcctc cggcctcctc
     4621 attcgcacct gcggtgctcg aactcctgcc gttccggcag tcgttccccg ccccaggggg
     4681 cgggggcagt catggcgata agcgacgttc cggtaggaac ggaacttgag caccggaggt
     4741 gcgtagccca atggaaagcc gtgccccggg ttgcaatcaa tcgagaatac ccggatggga
     4801 tctcagatta tcagttgttt cgtagggcac tacccggctt tgtcacctgc cgttcttccg
     4861 gatggcgtgc aggactccca tcacgccctt cacgtcatag ccgggcgtcc ggtcgatctc
     4921 aaaatcgtaa cgctccccga tgtagtcgag gagcgccctg ttcaccggcc ccccgatcgg
     4981 gggcatgtgg agttccatct cgatgcggcg cactccgtcg aggtcctggg gccggatgga
     5041 ccattccgcc ccctcgcagt cgcatttcag gaagtcgcac cctccggcca tggcggtgaa
     5101 ctcccccagg gtatgggtcg tcacggtgac ggtctcgccc ccccaggcga tctgctccgt
     5161 gcccccgtcg ccgagcgccc cgccgaccac cgtgacattg gcgccgttcg tgccgacgtt
     5221 gtcccggaga gcctccgtga ggacgggttc gacggccagc acacgccgcg accgccgggc
     5281 ggcacggatg cagaaagcgc ccacgttcgc cccgatatcg aggacgatat catccggccg
     5341 gatgtcgtcg aggtggtact cacctatgat gtagacggcg tcggcccccc gaggcatcct
     5401 cctgaaggtt acgccgtcgt gggtggtgac cggaggcggg cacggtaatt cgccgcccgg
     5461 caccgggacg tgttttctga acatgactcc ggataacgtt aggattccgg cctatttggg
     5521 cgtttctctc ccgtattccg gtggcgcttt ctcccatcgg agcccttgcg ctcccccgtc
     5581 ctgcagcgtc cggagggctc agaattctgt cacgttatta acctttttcg ccttgttcgt
     5641 ccgggagttg gatggtccga atccatatcc ggcacatata ggtggcttta tgggttaaaa
     5701 aaccacataa atgatttatt tgtcaataaa tatacttatc aatgagtggg tacagatgct
     5761 gtatgtagta agtctacaga atcttcagag gggagaactg cgatggcgta catggatgga
     5821 ataacctgca tttgcgataa cggccggacg ctcgaggacc accggcctat cgtcggcgac
     5881 gatgtcatct ccgggatcta ccgcaaggcg gcgagtctcc aggggaagcg tgtcctccac
     5941 gtgaactcga cctaccagag cggtggtgta gcggagatga tcgtctcgct ggttccgctc
     6001 atgaacgatg tcgggatcaa gacacagtgg aacatcttga acggcgacgg cgacttcttc
     6061 gccatcacca agaacttcca taacgcgctc caggggcagg ctgtcgcgtt ctcggacgaa
     6121 gagaaaggcc tctacgtcag gacgaacgag tccttctcga ggaggatgga gatcgaccag
     6181 gacctggtgg tcatccacga cccgcagccc ctgcccctga tccggttcta caacaagacc
     6241 cagccctggg tctggcggtg ccacgtcgac ctctccaacc cgaacgcgga cctctgggag
     6301 ttcctcaagg actttgtact ggactacgac cggatggtca tctcgcacga ggagtaccgg
     6361 cgccggggga tgccgatgga gcagtggatc tgccggccgg cgatcgaccc gctctcagag
     6421 aagaaccgcg acctctccga aggcgagatc gcaggccttc tcgcgaagta cggcgtgccg
     6481 accgacaagc cgctgatcac ccagatctcg cggttcgaca agtggaagga ccccgaaggc
     6541 gtcgtcgacg tctttgcccg ggtgcgcgag cacgtcgact gcaggctggt cctctgcggg
     6601 agcatggctc ccgacgaccc cgaaggctgg gcaatctacc gggcggtcga agagaaggca
     6661 cgagccttca tcgatgccgg cgatgtcatc ctgatcaccg ccgaggacca cctgctggtc
     6721 aacgccctgc agcgggcctc cgccgtcatc ctccagaaat ccctccgcga ggggttcggc
     6781 ctgacggtca ccgaagggct ctggaagggg cagccggtca tcgcatcccg cgtcgggggc
     6841 ataccgctcc agatcgagga cggcgagacc ggattcctcc tcgagccgac cgacaccgac
     6901 gggtttgcct ggagggtccg gcaggtcatc gagaacccgg agctcgggga gcagctcggg
     6961 cgggccggca aagagcatgt ccggcagaac ttcctgatca cccggctcct caccgactac
     7021 ctggatatgc tcaacgcaga actcgtgccc cagaacacct aaaaaagagt atacgatctc
     7081 aatatggcca tcggcgggtg ggaccgtgca cagcgatctc acggccgccc cccccggttt
     7141 tccggcaacg ttgggcctat ggacgcccgg ttttccggag gaggacgctt cgggttgccc
     7201 cgtcacgtcc tctgatgcag ggcggattgg cacagacggc tggctgagaa cccggacagg
     7261 agtttgcagc acaccttctt gtgaatatac tattttgtga atatgcgggt tacagggcag
     7321 attttcctgg atatttttga tggcgatgag actccggaag ttttagagat tgacccatac
     7381 cagttgactc caccaccccc ggacgatgaa gaatacgcat ccgcctatat aaccgtttct
     7441 cccggcaggg accggtgccg ctaccctggg gaatcccccg caccccggag ttatcgtgag
     7501 gtactccagc ctgccggcag ccggggaggt caaggtttct gaaaaaacgt tttagatgcg
     7561 tgacggttcg gttccgcggc ccttctggcg cggctcaagc cttccggtgc aacgcaccgg
     7621 cccgataaaa aaggatctcc ggggccggct gcaagcagag ccggcctgta ttgctcttac
     7681 cgtccgttcg gagatccgat gacctcgatc ggctgggtca gtctgactct cgcgatggtc
     7741 tttcctttca tcccttcgac gatctgttcg acggcatcgg agggcacgtc gacggtggag
     7801 taggtctcgt agatgttgat cgcaccgatg acgctgccgg ggatcccggt ctcgcccgcg
     7861 agtgcgccga caatatcctt cggcctgatc tggtgctctc tcccgagcgg gatcctgagg
     7921 agagccaacc cctgggccgg cctgagctcc tgcggctgac cctggttcct gtcgaccgat
     7981 ccggccccgg cgagttccag tttcaggagg gcagcggcaa catcgagcga cgagtagtcg
     8041 tcggatacga tccgctcgat gagcgtcgca tagtgctcga ggccgccttc gtctatgacc
     8101 tgctgcaccc gcttgatgag tttccgggtc cgggtctctt cgacgtctcc ctgtgtcggg
     8161 agcgggatgc gggcgatctt gatcttggtg tagtcctgga tcgtccggag cttgaagtac
     8221 tccttcgggc caacgaacgt gatagcccgt ccggcgcgtc cggcacgtgc cgtccggccg
     8281 atgcggtgga tgtagtagtc gatatcctgg gggacatcgt agttgatgac caggtcgacg
     8341 tcctcgacgt cgataccccg tgcagcgaca tcggttgcga cgagcatatc gatgtttcct
     8401 gcacggaact ttgccatcac ccggtcccgc agggtctgct tcatgtcccc gtggaggcct
     8461 tcggcgaagt acccccgggc ctggaggtgg gtggtcaggt catcgacacc cctcttcgtg
     8521 ttcgagaaga tcaacgtcag ttccgggtcg tacatgtcga gcagccgggt gaggatctcc
     8581 attctgtccc tgctccgtac ctcgaggtag agctgctcga cctgcgggac ggtcacttcc
     8641 cggcgtgcga ccgagatgaa ctcggggtct ttctggaacc gctttgagat ctcgaggatg
     8701 ggtctcggga gcgtggccga gaagaggacc gtctggcggt tccgcggggt ctcgtcgagg
     8761 atcttctcga tatcctcccg gaagcccata tccagcatct ggtccgcctc atcgaggacg
     8821 acaagcttca ccccggcaaa cgagacggtc ccgcggtcga ggtggtcgag cacccgcccg
     8881 ggagtcccga caacaacctg gaccccgcgc tgcagcgccc ggagctgccg gtcgatcggc
     8941 tggccgccgt agatcgggag gatgttgatg ccccggtggt acttcgccag gcgggcgaac
     9001 tcctcagcgg tctggatggc gagttcccgg gtgggggaga ggacgaggac ctgtgtctcc
     9061 ctgctgtcgg ggtcgacacg ctcgatcgcc ggaacgccga acgctgctgt ctttccggtc
     9121 ccggtctgtg cctggccggt cacgtcgcgt ccttcgagta tcgctggtat tgtgcttatc
     9181 tggatgggcg taggctcctc aaagcccatt tcttctatcg cccgaagcgc cttttgcgag
     9241 atattaaaat ccttaaaggt aatattgttc tccattcatt catctcttgg tgctgctact
     9301 tctttatctt gtgggggtga agggggcgga gcgggaagac cccacccttc agggtgggga
     9361 tgaaagcgaa gccgcctctg ttctgcactc aggcacccgc caacgtataa ccgttctgca
     9421 ggtgaaccta cagggatgaa ttgccggcat tcccccacga tgagcgccga aagcgttgcg
     9481 gaggtctacc gccggtacgc tgccgcgcgg ccggagtacc gtggggtcct cctccccgga
     9541 ctcctgccgc acgacgcctg cgcggcagaa cgccgcaggc ccgaccgcgt aacggtcctc
     9601 ttcattgccg agtctccgcc ctggaccgcc ggccggcgcg aagttgccga accttccgac
     9661 tgcttaagcc ccgactaccc ctacttctgg aacgaccgct acgacgcggt gtgccggccc
     9721 ggcgccgcgc cgctctcccg cgggctcgcg gagaacctct tcatgctgct cggcctcgac
     9781 ggggggtcgc gccgggagaa cctcgacctc ttcgccagga acttcttcct cgtcgacacg
     9841 gtcaagtgcg tcttccggaa gaaccggaag gcagccatac cgggcgacct cgtcaggatg
     9901 agcgcccggg agatcctcgg gcaggagatc gccggtctcg cccccgggta catcgtcgct
     9961 ctcgggaaca cggcgcttgc cggcctatcc gagatcgagc cctacgcgag cgccctttcc
    10021 ggcgtcggga agatcacgga gatcccggga gacctgcggg agagcctctt tctggagagc
    10081 cgcctcctct gcatgcccta cccgggcggg cgcaaccggc gctacctcga tacgatcgag
    10141 tcgggctttg agctcatcag ggaccttgcg gtatgacggg tcccccggaa agcccgtctg
    10201 catcgccgcc tacccggcga gtttcctctt tccctctttg tacaactctt ccttctgctt
    10261 acggagtttc gcggccatct cctgctcttc ggggcttcct ttatcttcga tctggatctt
    10321 ctgcgccgct tcctgtatgt cgcgggggct tgagtacccg gatggtttca ccttctccct
    10381 gtcctcccgg tactcctcct cgatctgttg ccggcgcatc tctgcggtgg actggccggc
    10441 ctgctcggcc tctgccccca ccgggatctc cttgccgcgg gcctcggcga ccttcttcag
    10501 ccgcacctcg aggacgccgt tcttgaaggt ggcgtgtgcc tcctcgcccc gaacatcggt
    10561 ggggagggag actgtccggg agatcgcgcc gaccctccgc tcccgtacgt gatatccggc
    10621 ctgctcctcc tctttcatct cctcccgccg tgcggtgatc cgcagcgtcc tcgggtcgat
    10681 gagcctgacc gatatgccct gcgtctcaac gccggggagg tcggcaacga ccacgacatc
    10741 tgcgtcgtgt tccatgacgt cgaccaccgc tccggtgccg ccgccgggca ggggcacctg
    10801 ctgtgcggct cccgagaggc gctccatcac ctcaccaaac tgcttctgca tacttccaag
    10861 catctcgtcg aattcgctcc agaactcact tgacgatctt ctccatgcca tggttaactc
    10921 cttcctgcct cactgttctg cagtatcggc aggctctcag ggtgtgggca cttcatatct
    10981 gttcctgtgc gggttccccc tgttggaatg acggcggttt ggggggttgc tccggggctc
    11041 ccggccgccc ccatggttac ctatagggag agtctccagt gggcggtgct atgaaccccc
    11101 agaccgtaga aacggtgccc cgggaacgct caggatccct gcctgccctc ggggtaatga
    11161 ccgtcatccc tctggagaac ctgaggatac acacctacga ggcgcccgag gcggcggtct
    11221 tcgtcaactc ccatatcctc gagacggagc acgggctgat cgtcatcgac acgcagtttc
    11281 tccgtcctca cgcagaagac ttccgcaggt atgccgatag cctgggtaaa ccgatcgacc
    11341 gcgtcatcat cacgcacagc catccggacc actggttcgg ctgcgagtat ttcagggacg
    11401 cccccatcta cgctctcgcc gaggtcgcgg accagatccg gggggcgggg aggccatgat
    11461 ccaggcatac gcaccagtct tcggcgatgc gatcacggag acggtggtgg tgccggagcg
    11521 gaccatccgg cccgggaccg agaccctcga cggcgtgacg cttgcgtacg agaaggccga
    11581 cgcggccgag gccggcgtca acctcgtcat cgagctcccc gagaaccgcg tgctcatcgc
    11641 ccaggacctc gtctacaacc gggtccatgt cttcctgcag cagaaggaga tgggcccgtg
    11701 gctcgcccat ctccgccggt tccaggagaa ggagtacgac ttcgtccttt cggggcacgg
    11761 gctgccggcg aacatgcagg cgctcgcaga tatggcgggg tatcttgagg acgccaggtc
    11821 cgcactcgcg gcgagcaacg atgtcccgac cctgaaacag cacctggtcg ggaagtatcc
    11881 gctctaccgg ggcgagatgc tgctggacat cagcgggagg tacctctatg ggacgtgagg
    11941 gatcccggcg aggtgctacc ttcggcgggc tacggccgtg gcggtgcaga tccggctgtt
    12001 attcttcttc ctggatcatc gcctcgaccg catctaccag aggcccgagg ttggtctgga
    12061 tgatgtccca gagaatcgtc ggtttcgccg aataatagga gtggacgctg atatcccgga
    12121 gcccggcaat ctttcgccat tcaatctccg ggtggcgggc ggtgacgctg gcgggatctg
    12181 ttttacggct tccccgatgg tgataaacct gagcacgacg gcatcgcggc acattgggtc
    12241 gcccgcaaac tcctcaaacg ttcggccccc tgcataggag cggatggagg caacggcctc
    12301 ccggatatcc tcgaggtaga ggaggacgtc cctaggcata gacgacctcg tcaaggacag
    12361 cgccccggat ccggggcttt aaggtatcgg caatgacgag atccgtcctt cgccccagga
    12421 ggtcttcgag gtagaacttg aggtccatga agtggtcaaa ggtctcctcc ccttcctgga
    12481 actccaccag aatatcgatg tcgcttgccg cggtggcttc gttccggacc acagacccaa
    12541 agacgccgat acgggccacg ccgtagcgtt cctggatggt gcgaaggttc tgccggatct
    12601 gggcgagggg gttcatcgtt acttctgggt gatacatcca tcctcttata ttctccggaa
    12661 cccggtcacc actccgccct cagaccgggg cgccgggccg cttcagcacg tagatggctg
    12721 tcccctcctc gaacccctcg ccgccccggg cctcgccggc agagacgaac ccgttcttct
    12781 cgagcacccg gatggatgcg acgaggtccg ggtaggtcgt cgccacgatc tgccggacac
    12841 cggggaggga gaagactgcg gggacgaggt ggcggaccgc ctcggttgca tagccccggc
    12901 gctggaactc ctcgagcacc gagtagccga tgaggaccgt gccgggaacc ggcgacgagg
    12961 tgatcccccc gctgccgacg aggacccgtt ccccttcttc cggatcgtcg cgcacccagt
    13021 accaggtgat gaagagggga tcggcgttct ccgcggtcac tcaggatagt tctaacagag
    13081 ggttcttact cgttgcggat ctcgatctgt ggggataccc gccacggtcc tgcatcaagc
    13141 ctcatcggcc ggcaggaccg ccgatatgct ccccgacaaa cgccaccgcc tcccccgata
    13201 gggtataccg gacgtctccc ccaatctttt cctggagcac gatgccgtcg gccttcagcc
    13261 gccgcatgtg ccacgagacc gacgaggcgg tgatcccgag ccgttcggca agttcccgcc
    13321 gcgacgcccc ggggctctcc agcaggatgg cgaggatctc ccgggtgttc gggtttttga
    13381 ggtggatgag caccttcgct tcgagcgccg agtacttccg ccggttctcg aagtatcccg
    13441 tccggccctc gaccgcggcg gcggcgatca tcccgaactc ctgcagtttc ccgaggtggt
    13501 accgtaaggt tccccggttt accccgaggt cctgagcgag agtgccgaga cggatcccgg
    13561 ggtgggcgtg aatgtggtcg tagatcgccg tccgggtctc gtgttcgagt gcggcgcgtt
    13621 ttgcaatccg gcggtagccg aagaagagcc agacgtttaa gagaaacagg acgttgacga
    13681 cgatcagaag ctcgggggaa gttccgatga gcaggctggt tatcagcacc tggggcggga
    13741 cctgccacca ctcgatgggc gtcagttcct ggggcgggcc gtcgggtatg tggtcgccgg
    13801 ccggcatgac cgtgtacccg gtggcggccg cagagacggg aagagcggcg agaaggatga
    13861 tgatcaggag agcggccagc caccatcgca tgctacagga attgcggtgc gggctcttga
    13921 cctttgcgct ttgccggtga ccatcacccc ccggctacgg ggaaaccggg gcctgtggat
    13981 agacagccgg cacagaaacc gtatataatt tcgtgtccca gattccacca tgatggatcc
    14041 ggaggaacag gcgcccgcgc cggtgccgcg tcccgggcga cgacgcatcg tcggcaccgc
    14101 ggccggcatc ggactccttg ccggggcagc cttcctcggg atggtggagg tgctctcccg
    14161 ctgctggagc gatccgtccg cctacaacca gggtttcctg gggttcggga tcacgcatgt
    14221 atacgtctcg atcctgctga tcatcttctt cgcggggttc ttctcggcgt acgcgaacgg
    14281 catcgccact cgaagcaccc ggatcctctc cgccgtcctc gccggcgcag tcgccgcaat
    14341 caccgcccgg acgctgctgt ttgtgggcga tcccgcctac ctcgtcgggt cgctcatcgc
    14401 gacccggggg cagttcctcc tcctcgtggg cggcgcgatg ctggtcgccg ggatcggggg
    14461 gctggtcgcg tcgttcctcg atccggagcg gccgaatggg cgccgggaac tcctcccgct
    14521 tgcggtggtc gccgtcgtgt tcatcgccgt cccgccgctc gttgcggtga tcggcatcgc
    14581 tgcgggcgcc atccccgccg cgccctactc cggcggcgca cccgcaccgg agaccgacgt
    14641 cctgatcctc agggtctccg ccgcaggcga tatcgagtgg gaggcgcggg tggatctcgc
    14701 cgcctacgac cggcccgacg tcctggccga gtgcccgggc ggttacgctc tcgcggtcac
    14761 ggagtacggc cgggagggga acacggtgca tatcctcacc tacgacgaag acggggactc
    14821 ccgcgaccgg ctggcgttcg agaccggatt cggccgcgtg accgcgctcg tcccggcacc
    14881 cggcggcggg ttcctggcgg tcacggagac cccggaggtc gtccgcgtcg atgccggggg
    14941 caggcaggtc tggatacagt cctttgtcga agagaaccac gggatggcac ccgtctccct
    15001 gctcgctcat acagacggac gatacatcgc ggcgtgggag gaccaggtcg cctgcttttg
    15061 cgacgatggt acgcggctct ggcaggtctc gctcgacgcc gcggggggtc tcgggtacca
    15121 cccgatctac ccggctcctg atggtggcgt gctcgtcttt gtagaggggc gaaacgtctt
    15181 tgtcggcgac catttcgagg tgtatctcca ggccgtccgc ctggatgaga acggcaccgt
    15241 cctctggaag cgggatttcg gaagcgttgg gctcgacgaa ctcctgggcg cccgggagac
    15301 ggcgccggga cggttcagcg tcctctaccg gtcgacgacc ttccccgaga atgtctgggg
    15361 cggcgttgaa agggcaaacc agggctacct cttcatgctc gacgaaagcg gcgagatgac
    15421 ggattaccat gccgtcgaag acgacggggg ggtcgtggtc gcgtcgtcgg gcgggtacct
    15481 ctcgttcact cccgccgatg cgggcgtcac gctcgccggc agggatgccg cgggcaacga
    15541 ggtctggcgg caggagcagc cgatcggtat cgatctctac tcgctccgtg gcatcggcac
    15601 ggcggacggc ggctatctca tcgccgggag cacgatcgca tgaagacacg gtggcttgcg
    15661 ttcggcatcg tcatcgtggt ggcgttcgtt ctctggcgtg cctcttacgt cccgcccggc
    15721 tacgaaacga tagactacga gaccgtcccg gtcctcccga cggagcggta caaccccccg
    15781 ctcctcgaga tctgggaggt gatggtggac gagattccgt tcgataaccg gacggcgagg
    15841 ggaccggggc tcattatgag tttcgacgca aacgggtcgt tcacctcgat ccagttccac
    15901 ttcttcgccg atatggaggg ggatccctgg gtccactccg ccttcgtcct cagaaacggg
    15961 agcgcctacc tttcgtccca gcggctcgat taccgccctc cccgcgcgca cccgcttgaa
    16021 gtgctcgccg ccgtcgacag catcccgttt aacgagatct cctacggcga gagggggatg
    16081 aacctcgcgg tcttttacca cgaggagaac cggtcctacg acgacaccta caaaaacatc
    16141 tacgctaccc tcgacggcac gctccgcccc ttaacggaga tctcctttgc cacccaggag
    16201 gtctggcaca ccatcgagat ctacccgatc caggagccgg tggtcatcat gctggacggc
    16261 gagccgcagg atgagaaccg aacggcaacc cgcatcgacg aggacccccg ggcgctggtt
    16321 gtctttgccc cgcgggagat cgggatggcg gagagggtcg tgtacgcgtg atgcgacgtt
    16381 ccggaggaac ggagcttgag caccgccagg tgcgagtaat gaccgctgtc ctggcaggag
    16441 tttgagcgtc gaaggtgcga cgaagacgga cggcacagaa aagatataaa tccctctccc
    16501 ccatcgctca tttaaggagt cgagcaatga gacgaaacct tccggctatc ctttgcattc
    16561 tcgcactgat ctcggcggca gccccggcga cggccggaga tacgacggtc gagacgtcga
    16621 cattcctctc cgtcatctcc ccggagcagt acgctacgat ccggagtgat ctcatcccgc
    16681 cccgcgccga ggtcgtcggc agggccgggg cttcgcgcgg gatacgggac gtggtggtcg
    16741 aaagcagcgt cgggaaggtc tcgtgcggga acggaaccga gtttgcctgc tccgtcccgg
    16801 tcgcggaggg gaacgagacg atcgccgtga ccctgatcga taacctgggg aagactactg
    16861 atgcggtgct gcacgtctac gtcaacgtcg acatcccccc accgccgtgg atctacgtga
    16921 tcgggacggt gagggacgcc gacggccgtc cggtcccggg cgcaacggtg cggttcgagt
    16981 ccgtcctccc gctcgccagc ggtccccatc ccgtgacgac cgggaccgcc gacgacggcg
    17041 gttacctgat agcgaacgcg ttcggctacg ggcagaacat atcggtcgag aaggagggct
    17101 atcagcccct gcaccgcgag gtctcctttg agaataccac caacaggctc gacctggagt
    17161 tggagccgca gggcccggcc gtgcccggtt tctccgcctg gatgagcgtg cttgcgctct
    17221 cgggagggct gctggccgcc cgtgcgatga gggggtggaa ggggtgaaag ccgatcccat
    17281 gcaacatgta cgaaccctcc tctgcatcct cctgctctcc agtctgtttg tcctccccgc
    17341 ggcggctgcc gagacccccc gcccggccga gagcccggtg gaggcgcccg tcgaccggat
    17401 cgagatcgac gacgcggtga agcaggcgac gccctactat gtcatgctcg tcgggaccga
    17461 cgatgagcgg gagaacctgc tcggctacat cgaccgctcc cctcgctccg ccgaagagaa
    17521 ggaggcgatg aaggagtccc tgcaagcgat ctggcggaaa tacccggtgg tgcacgagac
    17581 cgagggtgcg gttacccgga tcgcgttcgc cccggcggcg ggcgcgagcc cggtcctgac
    17641 cgacgaggag aacgcccggc ttgccgggat tgcccgggcc atggcggagg tgtatgccgc
    17701 cacgcccacg ggcacgatga ccacctggta cgacctctcc atctggctga tcaagacgga
    17761 cgggcagggg aacgaggcct ggaaccggac ctaccccgcc ggggatactg cggacgtcgc
    17821 gtcggtgctc cagacgaacg acggagggta cctgatcgcc gcgaacacag ggttccggga
    17881 cgccctgctg atcaaaacgg acggcgacgg caacgaggtc tggaggaaga cttacggcgg
    17941 gcctgaccgg gacagcgcgc tcgatatgat cgagaccgat gatgggaact acgtcgtcgt
    18001 cgggagcacc ggctggacac cggagaaggg tacgggcggc acctggatct tcaaggtcgc
    18061 gccggacgga cgacagatct ggagccggac gttctcggtg gaaggagaat tcctggtctt
    18121 ctcatcggtc gaccggaccg gtgacggcgg gttcatcctc ggcgggaaga ggaccacagg
    18181 agataccgaa accgacaatg cctggctggt caggctcgac cgggatgggt tcgaggagtg
    18241 gaaccggttc gtcgggggca acggttccgg gatcgtggac agcgtcatat cgaccccgat
    18301 cgacggaggc tacctggccg cggtgaggaa cccctgggaa gcggaacgtt ccgggaccgt
    18361 gccgatccgg gtgatcaaga ccgatccttc gggtgaggtc atctggacga agacgctcga
    18421 tgagatggac ggcggcacca gtgcggtcct tctcgcagtg cccggcggag gtgccgtcgg
    18481 ctatcttctc gcctgcgggg ccgtcctccc cgacccctgc ctcactaaga tcggcgggac
    18541 cggaagcatc gtctggaccg tgacgccgga ggttcccggc ggcgtccttc tggtcacgtc
    18601 ggccgctgca ccgtctccgg gggagtacgc acttaccggc acggtctacg ccggcgggct
    18661 gcatgatggg tggctggtgc tcctggacca aaccggaaac gtcacctcca cgcacccgtt
    18721 cggcggagac gcaaccgacg acatctcggc tctcgcggcg acggacgacg gcggctacgt
    18781 ccttgccggc accacccggt cgttcgggga gaccgggcgg acggcggcgc ccggccgtgc
    18841 accggggttc ggggcgctcg cggcgatggt cgcctgcggt atcgggctcc tctgctgcgg
    18901 ggggaagcgg gagtgaaggc cgggccgctt gccgccgcga gtgcggtctt cttctttctc
    18961 tgggcggtca tgccggtgac gctcgcatct catggcgccg tcagttcctg gagcgttata
    19021 ttcctgctgt ccgtcatctc actggccggg tcggttcagt tcctgcggac gatgcggcgt
    19081 gagtcgcccc ggtggtaccg gggcatcccg tttgcagccg tctgtactgc tcttgcgctc
    19141 tggacagggg tcgtggcgtg ggctgcacct taccatctcg cggaccgcgc cttccagctc
    19201 atgttcctcc tccccccggc cgcggcacta ctcctctcct ccttctcgga cggctgccgg
    19261 ggcaatccac ctcttccacc cgatcgccga gctggagacc gggttcgacc acgcgttcct
    19321 ggtcgggatc ctgttcatcg tcgggatgat cgttctgacg gtgcagctcc gggagagtga
    19381 cgtgagggag gaggcgagag agtgaccggg gcttaccccc gccccctccc ccagccgcac
    19441 gagaagtgcg cgataaaccc gttctgcccc gacgccagca cctcgtcggg cgtgccctcg
    19501 atgacgaccc tgccctcttc catgacgcag accttatcac cgagctcgag ggcgtcgctg
    19561 agcgcgtggg tgacgaggat gcaggggatg ccggcggccc ggatggtttc gcggagttcg
    19621 cggcgcatct gggcctgggc gcggacgtcg atcgcggcga gcggctcgtc caggaggaga
    19681 aggccgggtt tcaccgcaag cgcccgggcg agagccaccc tctggcgctg cccgccggag
    19741 aggcggccga cgttgacgtc cgcgaggtcg gagaggtgta tgcgctcgag ttcgccagcg
    19801 acgcgagcgg cgatctcgcc ggccggaacc ttccggcacc ggaggccgaa ggcgacgttc
    19861 tcgaagaccg agaggtgcgg gaagagcgcg taggactgga agaggtggcc gatgttccgg
    19921 ctctcgacag gcacgtcgac cctcaggcgg tcggagtaga gcacccggcc cgcaagggcg
    19981 atctcgccgc ggtccggggc gagcagccct gaagccaggt tgagcacggt cgatttgcct
    20041 gcgccgttct ccccgatgag ggcgagggtc tcccccggtc cgaccgagag ggagacgtcg
    20101 agttcgtagt cccgcaactg ttttacggca cgcatggaaa gcatcaggtg gccctccggg
    20161 tggtgtattt gaccgcgagg atcacggaaa acgagatgac cacgaggacg atcgcgatgc
    20221 tgatcgcgtc gttgaggtct ccctgcatcg tcgtgtagat cgcaagcggc atcgtctgcg
    20281 tccggccctg gaagttcccg gcgaacatga tcgtcgcgcc gaactccccg agcgcccggg
    20341 cgaacgagag gatcgccccc gacacgagcc cgccccgtgc gatggggatg gagacgcgga
    20401 ggaggacggc gagcctcgac gccccgagcg tccgggctgc atcctcatac agcgggtcga
    20461 ccgcttcgaa actcgcccgg gcctgccgga tgtagaatgg ggaggcgacg aagacctgcg
    20521 cgaggatgac cgcgaccgtc gtgaacgcga cggagatacc aaacccgtcg aggaactccc
    20581 cgacgacgcc ccggcggccg aaggccatca acagcgcgat gcccgcaacc gccggcggaa
    20641 gcacgatggg aaggtccgtg agggtatcga cgatctcccg gccgcggtac cgtacccgtg
    20701 cgtttacata cgcgagcggc gtccccagga ggacgacgac cgcggtgctg atcgaggcgg
    20761 tgacgaggga gagccggagg gcgtcgacga ccgccggctg ctgcagcgac gagagaaacg
    20821 cctcggggct gatccgcagg aagagcgagg ctaccggaat cgtcacgaag aggaggaagc
    20881 acgcgatgag gcagaagacg aggagggaga gggccgcctc tccggtctta cgggaccggg
    20941 tcgaatccat aggattccag gacggcgctg cccgtcggac cgcggacgaa cgcgatgaac
    21001 gcggccgcct cggtcttctg ctccgacgac gcgagtatcc cgagcgggta ctccgcgacg
    21061 acgttgtact ccgccggaac ctcgatctgc cggaccaggg agcgatcatt gggtcggacg
    21121 tccgacctgt agacgaacgc cgcatccgct tcgccgagcg tcagtttggg catcaccgtc
    21181 gtaatcgcgg tctcctcgga gatgacgttt tccatcacgg cgtcgcggta tgccgccccg
    21241 taggcggggt cggccgccat cgtatcgagc acctgccggg tgtaggaccc gaacgggaca
    21301 tccttcgtcc cgatgacgag tctgacgtcc ggccgggcga gatcggcgag gctcgtgatg
    21361 tttgccgggt tatcggcggg aacgatcacg gtgagcgagt tctcgaggaa gaccgcgacc
    21421 gtatcgttct ccatgaaccc tcctgcctcc agggccttca tctgctttac attcgccgag
    21481 acgaagacgt ccggcgaggc gccctgttcg atctgggtgc ggagcgcctg cgagccgtca
    21541 aaggcacagt cgacgacagt atccgggttc tctgccgtat aagcctcccc gatctcggaa
    21601 aacgctcccg tcagggacgc tgcggtaaag accgttagcg tcgtctgctg tgcggtcggc
    21661 tgcgtggtgc cggtgcatcc cgcaacggcg agcgttgcgg cgatgaggag aagcggcacc
    21721 aacagtgtaa ctctgtcgtg agatctcatc gtatgcctcg attgtacgct ggtttacgtg
    21781 ggtagggctc tgttgaaacc ctattctgat cttcatcctt accctgatgt ggctggtgcc
    21841 ggaccaggtt tgtccctggg actacctttc gtatgaaaat gggtcaccta catattttca
    21901 tccggtatgc ctgtggcccc acgggcatca tgtggttttt
//
LOCUS       DAJB01000045            4857 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_100952, whole genome shotgun sequence.
ACCESSION   DAJB01000045 DAJB01000000
VERSION     DAJB01000045.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_100952"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          828 a         1568 c         1483 g          978 t
ORIGIN      
        1 cgaaatggcc gccgcgttca agcaccgtcc atcgccgtat atgcttcagg ttgcgttccg
       61 catactcccg cggcggaacc gggtcgatgt ccctgggggg gagagcgagt cccaccggga
      121 cgtcgacctg ctggtccggc gcaagcgaac ccgagaccca ctcctcgcgg tagctgacgg
      181 ccctcaccga agggccgccc gtccagtaga gcatgatgtt cgtcagcagt tcgtctttcg
      241 agaagaccgt ctccaggtcg ccgtcggagt cgctccaggt gcggaacttc tcgatgatcc
      301 aggcagcgta gccggcaggc gagtcgttca atccgtaggc gagggtctgc ggtttcgtgc
      361 cctggagcac cgcatacgca ccctcggtaa acccctgcgc ctgtgacctc gcgaggtact
      421 cccgctccgc atccgagagg tccgggaagt tcgccatcat gatggggaag ccgatgtcgg
      481 tgagatggag gccgacgatc gattcggggt ggcgcacgcc gaggatctgc gagatcggac
      541 ttccaccgtc cccgccgccg gcgccaaacc gccgatagcc gagttcttcg gtcatcagcc
      601 gataggtcaa ctccgcaatc tttgcaagcg gctgtgttct gtccgacttc gacatccccg
      661 gatcgccgat cagcgagggc acgacgacgt cgaacgagtc ggcgggatcg ccgccgaagc
      721 gagccggatc ggtgagcatc gggatcagtt tgaggtagcg gcagatcgag tccggccagc
      781 cgtggaagag gatgagcggc atcgggtccg gccctttccc ccgctcgtgg atgaaccgga
      841 tcgccttccc gtcgatatcg gcggcgtagt gggcgaaccg gttcaactcc gcttcgcggg
      901 cccgccagtc gtaggtatgc tgccagtagt cgacgagctc cttcatgtaa ctcaggttcg
      961 tgccgtagtc ccagcccgca ccctcgactt cgtcgggcca gcgtgtgcgg gagaggcggg
     1021 agtgcaggtc gtcgagcgtc tcggcgggga cactgatggt gctcggccgt acggtcatgt
     1081 cgacgcctcc agagtccggt gctccgcacg caatgggcgg aagaacgccc ggagatcttg
     1141 aacgaagagt tccggctcct ccatcgccgt gaaatgcccg ccccgcggca tctctgtcca
     1201 ccactgcacg ttcagtcgtc gctccgccca ttcgcgggga ggggggatga agtctttggg
     1261 gaagaccgcc atccccaccg ggacgtcgat acgctccttc aggaagtcta acggcagggc
     1321 atgcatgttc tcgtagtaga ggcggaccga cgatccgatc gtctccgtcg cccagtagat
     1381 catgatattg gtcagcagtt cgtctttgct gaaacgccgc tcgatctcgc cgccgcagtc
     1441 gctccaggag cggaactttt cgatgaacca cgcggcgaga ccggcaggcg agtcgttcag
     1501 cccgtacgcg agcgtctgcg gcttcgtcga ctgcaccatg ctgtaggcgc cttcctgcat
     1561 ccacaactcc tggatagcag caagatacct cctttcgttc tccgagaggt cggggatatc
     1621 ggcaccgaga gagaagaacc cgacatcggt gaggtggagg ccgacgacct gcccgggatg
     1681 cctgagtgcc aggagctggg agacggcgct cccgatatcc ccgcctcctg cagcgaagcg
     1741 ctcatagccg agcacgtcgg tcatcagccg ggcccagaga tcggcgatcc gcgccgacgt
     1801 catgccgcgc tcgtgcggac ggtcggagaa cccgtacccg ggcatcgacg gaacgatcac
     1861 gtcgaacgca tcggcggggt cgccgccatg cgccgccggg tcggtgagca ggggaatgat
     1921 cgtgtggtac cggtagaagg agtcgggcca gccgtgcgtg aggatgatcg ggagcgggtc
     1981 ttcgcccctg ccccgctcgt ggatgaagtg gatgcccgag cccgcgatct cggtgcgaaa
     2041 atggtgatac cggtttaact ccgcttcgcg ggtccgccag tcgaaccggt tctgccagta
     2101 gtcgacgatc tcctgcatat agcccaggtt cgtcccgtag tcccatcccg caccctcgac
     2161 ctcgtcgggc aggcgtgcat gagaaagccg ctcccggaga tcgtccagga cgtgctcggg
     2221 aatatcgatg gtaaaagagt aaacctccat gcggggatta cgggaaacaa aggtaatagt
     2281 cctttcgcgc agccgtttcg cgtgaccagt cgggagaaaa caggcagaat ttatccgtgg
     2341 acggctgaag ccttcttcag cgtgaacaca aatgccgccc cctgctccgg gtgcccgggc
     2401 acccggtcct cgacccagac cttccccccg tagcggtcga tgaggatccg cacgaggtag
     2461 agtccgagtc cttccccgac gccccgcttc ttctgctcat accgatggaa gatcgcctcc
     2521 ttctggtcgt ccggcacgcc cgggccggta tcctcgacag aaacccatac agatccctca
     2581 tcttccccgg tccggacggt gatcgcgacg ccgggaccgc cgtgcttgac ggcgttgccg
     2641 atcaggtttg cgaagacttc cgggaggagg tcgtctgcaa ggaccaatcc tggagcaccc
     2701 acgtatcgga ttgcagcgtc cgggtagtga cataactcgt cgcggatgac cgcatccaga
     2761 tccatcgcct tgatctccgg cgatccggaa tggatccggc ggatggtgga gacggtcccg
     2821 aggatctcga tgctcttatc gatgctgcgc ttgagtttcc cgagatatgt ggcggcatcc
     2881 cccgagagca taccaagaag caggtcggca tagaggttcg cgacgttctc ggtgttcccg
     2941 atatcgtggg tcaggatgtc gagatacagg ttcgcctcgg tctgtgcctc tttaaggttc
     3001 cggctctgcc gggtgaggtc ttcggtgagc gtctgcagtt ccctgttggc gaattccaac
     3061 tgctcattgg cggccacaag cctggcattg gactcggcca gttcggcggt ccgctcctcc
     3121 acccggtcct cgagctcatc gtgcgctctc tgcagcgtct cctctgcctg cttgcgctcg
     3181 gtaaggtccg tggcgaccag ggagaagacc tgtattccgt ccatcggcag cggttttaag
     3241 gagaggtgca ccggcacccg gctgccgtcc tgcgcctgca gcgtgctctc gcccgtacta
     3301 ccgaacgtat ccgcctccat catccgccgg aacctcgccg tcccggcagg agcgatgaag
     3361 gcatggatcg actcccccat caccttctca agcggcatat ggagcagccg ggcgaagctt
     3421 gcgttgctgt aaaggatcgt gccgtcggcg gagaatagtg cggctccttc ccgcatctgc
     3481 tcgatgagga cgcggtaggg gtgctcggcg gtgtttaagg tgtagatctt ctccccgtcg
     3541 ctcgtggaga cgacgaaggc gtcgacctcc cccctccgga tcgccgaaag cgtctcttcc
     3601 gcctcaaaaa aacccccgta gagttcctcc agtacgcgga ggagctcttt ttcccgcggc
     3661 acccggggag cggtcgtatc ggatgactgg tctaaacctt gcatcgtgtg ttacctggtg
     3721 gacgaatcgg ttgcttcctt cttctggatt ccgagcccga tcagaacacg gtctttatcc
     3781 gacatatccc cgatcagttt ccggagaggc agaggcagct gcttgatgag cgtgggggtc
     3841 gcgacgacct gggcttcctg tgcccgttcg ggctgctggt agatgtcgat cacctcaagg
     3901 tcgtagcgtc ccgcgaggtg ctcctcgctg atctctttga tgttctcgat cgccttctgc
     3961 gatcggggcg tcattcccgt aacatagagc cgcaggatat agcgggcatc cgtcttctcc
     4021 agcgccagct cgaacgcatc cgtggccaaa tgctgatcct cgtcattctc gttctttctg
     4081 ttcgtcacac caaatcagct ccccggtcgc acgtccagcc cgacgaggac cttctccgtg
     4141 ttcgagaggt ccccgatgat ctttcgcaga ggcggaggca gcctccggac aagcgtgggg
     4201 acggcaaaga tctggtcgcc cctggcaagc tgggggtgct cgaggagatc gatcacctcg
     4261 atcctgtacc tcccggcgag atgctcctcg cagattctct ttaagttggc gaacgcggcg
     4321 agcgatttcg gcgtctggcc ggcgacgtag agccgcagga tccactcctc cccgttctcc
     4381 tcgctggtta tctcctctgt atcggtcatc gcgcctcccc ggcctcccct cttgttgcct
     4441 gaaccttttt atcatcatcg gaagcatcgg ctttccgcag cttcccgatt gcccgggtct
     4501 ccacatcaat cgtacgctgc cggcggctct ccgctgcgat cagtctccgg acggtctcct
     4561 cctgctctgc gaactcactc tggagggcgg cgatccgggc ttccacggca gtccgttccc
     4621 gctccagttc acactgccgt gacgctatct cctctcttcg ggcctctgcc tctgagcgct
     4681 ccctcgcctc ctgcacgagc cgggcggtgc ccatgaacat cccggcaggc ccgacgtaga
     4741 cgtcggcaag ctcgatcccg tcgccggtga tcaggtattc gcggatctgg ttggaatgcc
     4801 ccatcccccg ggacttcagc acgtagagtc cgcggttgcg ctccccggca gactcga
//
LOCUS       DAJB01000046            5801 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_71641, whole genome shotgun sequence.
ACCESSION   DAJB01000046 DAJB01000000
VERSION     DAJB01000046.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_71641"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1202 a         1767 c         1645 g         1185 t
ORIGIN      
        1 tcgtgttcct tacatacgcg aactggtcgc ggatcctctc cgggatcgtg cggggatctt
       61 ccgccataac ggcctcccgc catccctcgt cacggcacga gcagtccagc ccgtcgaact
      121 ccgtgatatc gaagtggcag ttgtagcgct cgattgcctt tgtgatctcg gcatcgcact
      181 ctccgcagtt ccgggcccag tcgagggact cgaccgagta ggtccaggga ctgacgtaga
      241 cgtgggcacg atctccaaac cgctcgtggc actgctgcag aaggtcgacg agactccaga
      301 gccacggcgg gcggtagagg ttattcttcc agagttcgtg cacgacggta ttcttctcga
      361 ctgtcgtcac ctcgatgtcc acatagtcga tcccgcggga tacgcagtac gccacggtct
      421 gcaggacgtc gtttatcgcc tccgcctcgg tgaggaacgg cggttttacg agcaggagcg
      481 gttttgtcag cacgcccata ccctgcatcc gcgaatggaa gagatcgaag ttattggccg
      541 agaacccctt gttgatgcag aggttcatca cctgggggtt tgcggattca aacccgaacg
      601 ccacctcgag gtgccgcccg ttcagcgcct tcttcatctt ctgcacgtgc tcatccggcg
      661 agtaatcgat cctcgactcg acgacaacct tcttgatgtt cttgtagccg tcgatatacg
      721 agcagatctt ctcgatggca gcgaccggca tctcacgctc gttaaagata tttccatcgt
      781 tgtataccga gacgatcggg taatccccca gatcgacggc gtccatcacg tactcgaact
      841 gcgctacgta ctctttcgcc gaaatctccc tgccccgtga ggttgcaccg taaaacccgc
      901 acatggtgca tcccccggtc ttctcgaccc actcgcagcc cgtggagcgg aggaagatga
      961 tcagccgctt gatgggcaga ccatcaaggt gccccgtccg ttcctcccag ccgatgggaa
     1021 cagtgaaatc gaggttttgc ggggcgtctc cattctgcct gcgcaacccg ccggccagag
     1081 cccgtatctc gtccagaaca tcattctcgg tcgggagacc cttgaagatc tccgcattgc
     1141 tactgctatc aggtacggcc ttctctagaa taattatccc ccctgccccg ggcatgcacc
     1201 ccaatatcga agatacacgg ctccggtgac tgtcgcctta tcttagcgag acatacccct
     1261 gataggcttc cgcatattta tagtttatcc aaatcatcac atcaccgggc catgattgtc
     1321 aaacggtatt atctgacagg aagggaaggg aatcggaccg cgcgtcaaaa agagccggat
     1381 ctgcacccca ctataccctt tcaggagagc cattcgtccg gttatagact cctgataccg
     1441 aatggagcag atgacagcat gccaattttt atatacgttg tttggggcta tactatctcg
     1501 atacaccggg ggatcatcgc ccgtgggggt agtgcccccg caggcttatc gggagagaca
     1561 atccggtggc gacggcgagg caacgaggcg gtataacggt gccgtcccgg tatgatagaa
     1621 gagtctgaaa aggagggaga agatcgtgat acgcctggga gagaggatct gtggtcggga
     1681 gtcgttcaca acaaaagccc ttggcatcct tcctgcgtat aatgtctacc gaaagaccta
     1741 cgctcttctc cagcagtccc ggcagtggag cgaggaagaa ctcgcagcct accagacgca
     1801 ggcgctctcg cgcctgctcg accatgccta cgagaacgtc ccctactacc gccgggtctt
     1861 tgaggagcgc cgtctcgtgc ccggggacat ccagactccc gccgacctcg ccctcctccc
     1921 cttcctgacc cgcgaggacc tgcagaacaa cctccccgac ttaaaagccc ggaactaccc
     1981 ggagacagcc ttcgagtacg tcaccaccgg cgggtccacc ggcattcccg tcggcttcta
     2041 ctacgagaag ggggtctccc gggcccggga gtgggcgttc atgaagaccc agtgggaccg
     2101 cgtcggctac cggttcaccg accggtgcgt cgtcctccgg ggctacatcg tcgggtccgc
     2161 ccaggacggc atcttctgga agaagaccct cttcgggcgg tggctgctga tgtcctccca
     2221 tcacatgacc gaagagaccc tcccggccta catcgaccag atccgaaaat tccggccccg
     2281 cttcatccag gggtacccct ccacggccgt gatcctggcc aggtatatgg tggaccacgg
     2341 catcgggccg ttcccgaccg tcaaagcggt cctctgcggc tccgagaacc tctacccctg
     2401 gcagcggagc ctgcttaccg agaccttcgg gtgccgggtc ttctcctggt acgggaactc
     2461 cgaacagacg gtgctcgccg gcgagtgcga gaagagcacc cggtatcata tcttccctga
     2521 atacgggatc gtcgaactga tcgggagaga cggcctgccc gtggaggggc cgggcgcgat
     2581 gggggaggtg gttgcgacaa acctcaccaa cttcgtctgc cccctcatcc gctaccggac
     2641 catggacctc gccacagcag ccgggggggc ctgcacctgc gggcggagtt acccgctgct
     2701 cgagcgcgtg gaaggccggg ttcaggactt catcgtgacg ggcaaggggg agttcctctc
     2761 cgggatcgcc atgaacatcg ataccgacgc gttcgataac gttaaacagt ttcagttcta
     2821 ccaggagaag atcggggaag tcgtcctgaa catcgtgaaa aaaccggcat tcagcaaaca
     2881 ggacgcggaa tatctctatt gggaagtgag ccggagctgc ggtgacgatg tcgtcatcac
     2941 gatcaggtat gtcgatgaca taccgctcac cccacgcggc aagcaccgct acttcgttca
     3001 ggacctacca atatgcttca gcgaacggaa gggggtgaag gttgagcatg tatcatccga
     3061 tatattcacc tgaccaggga gatgtgcatg tgaaggcagg ttttttgagc ggggcatctc
     3121 gtgcgaatcc actcaccata aaagcgctca gtctggtgcc gtcttaccat acctaccgga
     3181 aaatgtatgc gcttctccgg cagtcccagt ggtggagccg ggaagannga gcgccacctc
     3241 gttcccgggg atatagcgac ccccgccgac ctcgccctcc tccccttcct gaccaaagag
     3301 atcgtccagg ctaacctcgc cgacttaaaa gccagaaact acccggagac ggccttcgag
     3361 tacgtgcgca catccgggtc caccggcacc cccatggggt tctactacga gaagggggcc
     3421 tcccgggccc gggagtgggc gttcatgaag acccagtggg accgtgtcgg ttaccggttc
     3481 accgaccggt gcgtcgtcct cagggggcat gtcgtggacg cggcatcaag cggcatctac
     3541 tggaagcggg cgctcttcgg gcggtggctg atcatgtcct ctcaccagat gaccgaagaa
     3601 gcgctaccgg cctatatcgg ctggatccgg aagttccggc cccggttcat ccatgtctac
     3661 ccctctacag ccgtgatcct ggcccggtat atgagggagc atggcatcga gcccttcccg
     3721 accatcaaag cggttctctg cgggtccgag aacctctacc cctggcagcg gagcctgctc
     3781 accgagacct tcgggtgccg ggtcttctcc tggtacggga actccgaaca gacagtgctc
     3841 gccggcgagt gcgaggagag tactcattac catatcttcc ccgaatacag cgtcgtcgaa
     3901 ttgatcggga gagacggcct gcccgtggag gggccgggcg cgatggggga ggtggtcgcg
     3961 acaaacctca ccaactttgt ctgccccctc atccgctacc ggaccatgga cgttgcggtt
     4021 gcctcaaaaa agcagtgctc gtgcgggaga cagtatcccc tgctcgagaa ggtggagggg
     4081 agactgcagg agttcatcgt aacccggagc gggaacctga tctccgtcac ccccatcaac
     4141 tatgaatcgg gagccttcga gaacatcaga cagtttcaga tgtaccagga ggagatgggc
     4201 gaactgatca tgaaagtcgt cagaaagccg gcctacaccg aggaggacac ccggcagctg
     4261 acgagggaac tccagtggca actcggcgat gaggtgaatg tccatgtccg cttcgtcaat
     4321 gagatccccc gcaccgaggg gggcaagttc cggtacctta tccagaaact cccgatctcg
     4381 atcggggacc tctgacggat cagtcgatgt aggagacaac cctgcgtttc agccagggat
     4441 tattgacgac ggaactgtag gcccattccg ccagggctcc gaccatatcc ttccggcaga
     4501 cctccatata catgcccagg tccgagcaga accgggattt gaagaagttc aggtccggcg
     4561 tgtttgctcc gatattctcc agaagtgggt atccttcact ttttgcacgc tggatcagga
     4621 gccactgaag gcactcgttt ccggcatgag cgctctcgat ccgcggcgta cccatccaga
     4681 gcaggaagcg tttgtactcc tgggtggcga cagcccccgt gagcgcacct tcccggtcgt
     4741 agaggtagta ggccgcgaga cggtcggggt acgcctggaa gaggtcctca aagtagcgcc
     4801 tgctgaccat cgggatgttc atatccggct ggctgaaacg ctcggcaagc gacgcataca
     4861 gcaccgaaat atcggtgctc cgcacgagat ccataccagc cttctcggcc ttccggatct
     4921 tgttgcggag tttgtagtgg aaaccacccc agatcgcctc ggccggcggc gagaggtcga
     4981 tcatgtaggt gtagtggacc ttcggggcgt atcctctcca gaggaactgt ctgacatcgg
     5041 cgaggccggg gaccagtacc agagagaggt agtgcggcga gattgcgtcg atctctgcag
     5101 agagatcctt cacaatgagg tccatgactg cctctttctt gcccagtttc agtgcatcaa
     5161 actcttttgc cgggatgagg ccctggtacg ggatgaccgc ctgcatggga ggcggcgaga
     5221 agagcatctt taccccgtgt gtgttcctga agtagagcgg agcgaggcag acaagttcct
     5281 cccccttgta gaccccgtaa gggaggagcc gataccccgt atgctgcgcg gtgatcgtta
     5341 tgtagtccca cttgtggaaa agcagcccgt acgggcttgc gtctacgaac gaatcccaca
     5401 cttccctatc tttaatgagt tcgagcacca tagagcctga cttccagccg atcggtcatc
     5461 ccagaccgcg tccgctacag gatacctcaa caggtgtatt tataatcttc cctcaccgcc
     5521 ctccactcgt gggggcctgc acctttctcg gggagttcag atgggtccgg ggccccatgg
     5581 tgtcctcctc ctgcatgcgt ccatttaatg gaggagaccg ccccttaacc cacggcctgc
     5641 cgacgatcat ttaactctat gaatttcctt tcccgccctt cacatctctc acggcctgcc
     5701 ttctgtgtgg aaccatatcc cgatctgcac catggattgc ttcacgcgaa actacgaaat
     5761 ttcagttctg ttgaataggg catgaatatg gaagcatcga c
//
LOCUS       DAJB01000047            7669 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_119338, whole genome shotgun sequence.
ACCESSION   DAJB01000047 DAJB01000000
VERSION     DAJB01000047.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_119338"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    4989..5028
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1460 a         2341 c         2467 g         1361 t
ORIGIN      
        1 tccacggtcc atcaccacct gcagaaactg gccgactccg gttttgtcgc cgccggagac
       61 gacgggcacg cctgggtgta ctacgcgctc acgccggagg ggcaggccct cctgcagccg
      121 cacggcggcg cccggatccg gatcctcctg gctgcggggc tggcaagcct cgccggcggg
      181 gtctgtgccc tcgcggcctt cctggcagcg ccgatccgtg aggggccgag accgatgggc
      241 ggcggcggcg gcctcccgat cccggagggg cctcccacgg agctgctcgt tgccgggatc
      301 gcgctcgcgg tggtcggggg agtcctcctc gtagcgtatg cctatgtccg gtggcggaac
      361 agacgtgcgg cgggggactt ccctgtggac agaaaagtca cggaacctct ataggtagtt
      421 ttcggatccg ttcgaccggg atcgaacggt ttcgctgatt ttatcgcacc tggggagtgg
      481 aagttagaac aatgaaaaca gatcttttct cgaagatttg cgttctgctg gttctggcat
      541 cgctcgtcgc actctgctgt atggtcgcgg ggtgcaccga gatgccgggg acgacgtccg
      601 caagtaccgc ccctcatgcc agaggggaga cgccggacgg cgtcgataat gtggcggccg
      661 ggaacaaccg gttcgcgttc gacctctacc ggcgcctcgc ggccgagccg gcgcatgcgg
      721 gagacaacct cttcttctcg ccctacagca tctcgtcggc cctcgcgatc acctgcgagg
      781 gcgcccgcgg cacgaccgcc gacgagatcg gagcggtgct gtatctcccg gagaacgaca
      841 gcctccggcg ggaggggttt gccgcaatcg atgccggcct gaaccgcggg agcggcaact
      901 acaccctcca caccgcaaac gccctctggg cggaggagac ctacccgttc ctcccggcct
      961 acgtcgatgt ggtcgctcgc tggcacgggg caaacgtcac gaatcttgac ttcatcaatg
     1021 accccgaggg gtcgcgggag acgatcaacc ggtgggtgga agagcagacg gaggataaaa
     1081 tccgcgacct cctgccgccg ggttcaatcg accccctgac ccggctcgtg atcacgaacg
     1141 ctatctactt caagggcacc tgggccgaac tgtttgaccc gaacaagacg acggaggagg
     1201 agttccgggt tgcaccgaac cagacggtga ctgtcccgat gatgcacggg aatgccgtct
     1261 atccgtacgc agagactgag accctccggg tgctggagat gccgtacgcg cacgggaacg
     1321 aaaccgaact tgcgatgctc gtccttctgc cgaaggaaga caacctgacg gctgcggagg
     1381 aggcccttga cgccgggcga ctcgcggatc ttcagaagtc cctggtctcg cagagggtca
     1441 gggtcttctt cccgaagttc acgctggaga cggggtatcc tcttccggga gtgctcgcgg
     1501 cgatggggat gccgacggcg tttgccgacg atgccgacct ctccgggatg gacggcacga
     1561 gagacctctt cgtcaccggg gtcttccaca aggcgttcgt cgacgtgaac gaggagggta
     1621 ccgaggctgc ggccgcgacc ggcgttgtgg tggggggagg tgccacaccc gtcttccgtg
     1681 cggaccaccc gttcgtcttc ctcatcgttg agaaggactc cggcgcgatc ctcttcatag
     1741 ggagggttgt caaccccaaa ggcacgtgaa gggggctgtt cccctccgtc gtcaacggat
     1801 accggaggag ggaggggcgt tctacccggc gtcttctgtg ccgccgagca aagagaggag
     1861 cgccacgacc tccgccctga gtgcggggat atcgtggtcc acgaccgccc agacctcatc
     1921 agggtccacg cggaagtatg cgtgaaccag gatatttcga agaccgatta cagatccggg
     1981 gttcaagggg tgcaagaaag cccccggctt tagccgtggg gatgaattgc accccttggt
     2041 ccgttcactt tcaccccgcc cgggatagat tatagccgga tgagcactca cggatctctg
     2101 gagtctggaa tgcggcacgt cacccggtac cgagcatacc cctctcacac cgttgagcag
     2161 cgactgttca ccggctttgc gaggtgcgcc tttgtccgga actggtgtct tgagaacagg
     2221 gtgtaccggg actcctgtct cccgaaactg aaagccgatt accccgagtt gaaggaagtc
     2281 cacagcaagg tgctgcagaa tgtggttcag cagatcgtcc acaacctccg cgctctcaga
     2341 aaactgaagg aaaaagggag gaaggtcggc aaactccgga agaagtgggt tcactcgatg
     2401 atctacgagc agacggggtt caaactcacc ggcaaccggc tctggctgag caagatcggg
     2461 gagatgcgga tcgagctcag ccggccggta ccggggaggg tcaagcagat cgtggtcaag
     2521 cacaccccgg cccacaaatg gttcgtggcg gtcatctccg agacgcccga taccccggta
     2581 ccgacgaccg gcacccgggc ggtcgggatc gacctgaacc tggagaactt ctccaccgac
     2641 accgacggca gggtcttccc gcacccccac aacgtcagga cggccgcaaa aaggctccgc
     2701 cgggcccaga gaaaactctc ccgggccgcg gacgggagcc ggaaccgccg gaagcagcgg
     2761 gtccgggtag cccggatcca cgagcaggcc gagaaccgga ggaacgactt cctgcacaag
     2821 tggagccggt attatgttga tcgctatgat cttatcgctc tggaacacct gaacatcaaa
     2881 ccgatggtcg aatcgctgga tgcaaaactc cgcggcagat ccaagtctat tctcgatgct
     2941 gcgtggtaca aggcaagaga gttcatacgc tacaaagctg cgaatgctgg taagtatgcc
     3001 cacctgatcg atccggccta cacgacgcag gactgtttcc tttgcgggca ccgggagaag
     3061 aaagatctct ggcagcgaac ccatgcctgc ccggtctgcg gctacacggt accccgggat
     3121 ctaaacgccg cacaaaatat cctgaaacga gcatccgtag ggtggggcac acccgaatct
     3181 acgcttgtgg agatcgggac cgctacaccg ccaagcccgg cggtgcaagt cccggtcaac
     3241 gaaacaagaa tctcccggct ttagccgggg agagtgtcaa ctgtttccag ggaaccccgg
     3301 gagaagtgct tcgcatgtcc caggggagac gtgaggctgc ttccccgatg atctggaggt
     3361 gatgcaccat ccagacctgc accagttcat cttgcaaaaa agcatctctg ccccggaccg
     3421 tataccgctc aatctcctcg atcgcttcca ggatatcgag gagccggtcg atcccgtccc
     3481 tcataacggt actgcctctt tgaggatccg ctttttgtac cgctccttga gcgccgtttc
     3541 cgtcaccacg tccacccggc acccaagggc ctcctccagg tcctggatga gcgcgatatg
     3601 gtcgaggagg ctcctgccct gctccagttc caccagaagg tcgagatcgc tctccgggcc
     3661 cgcctcgtcc cgggcgaccg acccgaagac ccgcagattc ctggcaccgt gccgcctggc
     3721 tattgcgaga atcctggacc gcttctcccg gatcagatcc tgtgtgacgg gcattgctgt
     3781 tcaatatctc ctatcgggag ctggagatat aaatttgccc ccgatgaccg catcgtcaaa
     3841 aaaacctccg gcagggcaag ccctcactat gggaacggcc gtcctgccct ccccgatagc
     3901 gtcgggcaga caccctctca accgaccggc atgcccctgc aggagaccgg agcacgggcg
     3961 gggtgcggga aaggccgctg acaagagtcg caaagatgct gtgttcgaga atacaccatt
     4021 ctcctcacga ggaccggagc cgggagagga tccggagctt ctttgcaagc gccgcctccg
     4081 ccgcccgtgc gaccccgatc ttcatctctt caaaatccct tttgtcctcg tccacgacct
     4141 ttttgaccgg ggtgtaggcc gactcccgga accggggcgt gaagtcccgg atctcgccgc
     4201 cgaccgagag gaccgattcg ggcactcctt cctcgacgta gccgacctct ttcatcgcga
     4261 cgcctgcaga ctccacgacc cgccggatct cgggcgcgac ctccggcggg gcgacgacca
     4321 ggagggcgtc gagcgatacg ccgaggtagt cgatctggag cgcgtcgagc atcttgagga
     4381 cgttgggctg cacgagcgtg cggagttcgt cctcgacgac gacgatccgg cagccggcgg
     4441 tctcggccat ctcgtaggcg tcgccccgga gcccgccgtt cgtgacgtcg gtcatggcgt
     4501 ggatggcggg gaggacgtcg cttgcaagga gcgcctcgca ggccttgagg aagtggaggt
     4561 tgatcgtctc ctcgacgacg tcggggaacc cggagtagat cgcggcggtg gcgatcgtcc
     4621 cgccgccggc tccttccgtc atgaggagga cgtcgccggg ggcgatctgt ttcctggcgg
     4681 tgaggtggct ggcgacgccg acggcgccga cgcagccggt catccgggaa ccgagcacca
     4741 tgtccccgcc gatgcggagg gtggacccgg tgacgagggg aacacccatg gcctcgccga
     4801 cggccgagac tccagcggtg tagtcgaaga tcttcgcgac gtcgccgtcg tcggcgacgt
     4861 ggatatcgga gaggagggcg acgggttttg cgcccatcac gtagacgtcg cggagcgtcg
     4921 cccgggtgac gtggaacccg gcaaggaagg ggaagtcgga gaggcgggag tgcatcccgt
     4981 cgacggtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gccggtgggc
     5041 gaagaagtcc ccggaccccc gcgacccgac gccgaactca cccatcgtcg agccggagga
     5101 ctcgaaggcg aagaggtcgc cggcaagccc ggtcgagttc ttcgcttcgg taaggaccgc
     5161 ccgggcgaat gcctcggcgt aggcaggggt cacgcggcgg tgttttatgg agagcacatc
     5221 ccgggcaagg atgcgcacga tttcatcgtc aggagcgccg gcagcgaggt gccggcggct
     5281 gatctcttct acatccattg taagagtggt cggtgcagga ccagataaaa ggagtggcgg
     5341 ggggcaggca aagattcaag cgggtctgcg ccgacaattg aagaatgacg ggtgcgcaga
     5401 tgatcgcagc atgctctatc gccggctccg actccggggg aggggcgggg atccaggcgg
     5461 acttgaagac gtttacggcg ctcggcatct gggggctgac ggtcataacg gcggtcacgg
     5521 cccagaacac ccgggaagtc cggggaacct ggatgctccc ggcggatgcg gtgcgggctc
     5581 aaattggggc ggtcgccgac gacttcacca tcggggcgtg gaagaccggg atgctcggca
     5641 atgcccaaaa cgtccgggcc gtcgcggagg ccctgccgga gggagccacg ctcgtcatcg
     5701 acccggtgat ggtctcgacg tcgggccacc ggctgctcga acaggacgcc gtcgcggacc
     5761 ttacggggct gctcatcccc cgggcggcaa ttgtcacccc caacatcccg gaggcggagg
     5821 tgcttgccgg gatgaacgtc gtgaccgtcg aggatatggc cgaggcgggc cggcggatcc
     5881 tggatctcgg tgcccgtgcc gtggtggtga agggggggca tctcgccggc ggtgcagcag
     5941 tcgacctcct cgtcgacccc gacgggatga tgcggctctc agggaagcgc tatccctatt
     6001 cggttcacgg gtcggggtgc tgcttctctg cggcgctcac ggcatacctt gccgggggaa
     6061 tgcctgcgcg ggaggggttc gccgcggccc gggagttcat tgatacggcc atccgggaag
     6121 cggccggagg ccccgggccc gggagggtcg tcaatcccgg gggaacaatt attcaccggc
     6181 actgaaacaa aaattccccc atcatacaca atttcgatta ttatttcctc tttatcgcgg
     6241 aataactctt catttatcac cggacagaac tctgacagaa tagctgtaat tcgcaacaat
     6301 acccgcccga aagaggaaat accgttctct ctggtaaaaa caaatgcgga attttaataa
     6361 ctaaaaacct gtaactattg taagaatgga tgagatcgac gatgcgatcc ttcgggagct
     6421 gcagagagac gggcgaatgt ctatggcaga gctcggctcg atgctgggca tagcaccgtc
     6481 gacagtcttc aaaaggatcg agaagctgaa gaacgcaggt atcctcgaac ggttcacgat
     6541 cgtcgtcaac cagaagtgtt tcgagcatac cctggtcgct tttttaaacg tcagggtcca
     6601 cccggatgga aagcctgagg tcgaggcgtt catgcgggac ctcccgagta tccttgaact
     6661 ctacgaggtg cttgagcccg gggacttctt cgtcaaggtc cgggtgcaga acatatccga
     6721 gttgaagcgg agcgtgctcg tccccctctc cggccttccg ggggtgaagg atatccagac
     6781 gatcatctcg gtgaggaagg tcaaggaaca gacatgagcg gaatacagat tgaaaccatg
     6841 cagcagatgc ttgcgattgt gacgctcctc ctcggagcgc tcctcatcgc cgtcatctac
     6901 aatgcctatc ggatcgtgag gcagccgacc ctgctcctct tcatctacgg gcttttcacg
     6961 ctgatcgtgg ggatcacgtt tgctgatctc gtgagcatct tcacgcctga tgagttcatt
     7021 gtcctctggt cggcggtctt ctcgcgtatc gtggtggtct taggcctctg tgtgatggcc
     7081 tacggcatcc tgagaggata agatgcgcac cggtacttcg tttgcccggg actggcagtt
     7141 gatcaaggta gcccggtccc tgcggcggca cgacgtcgcc ggaccgctcg tgaaaagact
     7201 ccttgcggac gcctcccccg gccttacgga gcggatcacc gtcatcgccg ggcggctcgg
     7261 cgaagagaac ggaacggcgc ttctcgccca caccgaggaa cggttcgatt ccccaaccct
     7321 gatggaaggg ctcctcctga cgtgggggat cccctgcgag tccacggacg ccgacgacgg
     7381 gagcgtggtt atcgccatcg gcggcgacgg cgcagcagcg gcgatccggg agaccttcgc
     7441 agacgtccgg gtggccgggc cttacctcgc cggttacgcc cgggcgctcc agcgggacgc
     7501 ggtgctcgag cccggcgccg gcggcagtat gacgatccgg ttcccgcctc gcggggagta
     7561 gggcggggct ggtttttgac cggcggttcg gacgcactct ctcgccccgg gggtccggga
     7621 cagggccgct gctctcgcat ctccggccgg ctacagcaga agcacaggg
//
LOCUS       DAJB01000048           10300 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_109169, whole genome shotgun sequence.
ACCESSION   DAJB01000048 DAJB01000000
VERSION     DAJB01000048.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_109169"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1796 a         3278 c         3416 g         1810 t
ORIGIN      
        1 accccgacgg cccgaccagg cagataaact ccttgtcccc gatatcgagg ttgacgccct
       61 cgagcgcctg cgtgacagtg ccgtcctcct tggggaaggc tttgccgagg ttccgtatcg
      121 taactccgcc cattctaggc cacctcccct gcatgccacc gcaggagctg tctgtccaca
      181 tagatcctga agatgcggtc gatgatgagc cctgcaaagc caaggatcag catgtagacg
      241 acgacgttgg gcatctggtg gaggtagtag ttatgccaga gctggtaccc gaggccgtaa
      301 ctggagacgc cgaacatctc ggcggcgacg aggcacatcc acccgacgcc catggcgatc
      361 ctgatgccgg aggcgatcgc cgggacggcg gcgggaaggg cgacgtaacg gatcagctcg
      421 agggaggagt cgcacccgag caccttgccc gcctcgacaa agaccttcgg gacggcccgg
      481 aacgcggagt aggtcgcggt caggatcggg aagaacgctc ctgcaaagat gacggagcct
      541 gccgcctggg tggtgagccc gaaccagatg atggcgaacg ggatccaggc gagcggcggg
      601 atgggacgga ggatctcgat gatcgggtcg agcaggaaat ccgccacccg gaaccacccc
      661 atgaggatcc cgatggggac gccgaggagg aacgccgcaa tgagcccgat cccgaagtgt
      721 tcgaggctca ccatggtgtc ggcaagaagg gtgccggagc cgaggaggtt cgcgaatgcg
      781 ccggcgacgt cggtgacgct cgggaggatg aaggatcgcc ctaccacgta ttcggcgatg
      841 atctgccaga ttactgccac ggcggcgagc gctgcgacgc cgaggagtcg tttctgtgtc
      901 tgtaggttca tgtctctatt ccattgcctg ggatcttgca gaaggtgggg cgaaggcctc
      961 ttcgctccgg aaggttctct cttatttcat cggcacgtct ctaaaagata cttttggcgg
     1021 gaaaaacggg tagggttccc gggttacgcc cgggccttct catagaacga gaggtcgaag
     1081 aggtcgtcct ctgtgagggg cttagtgatg tagccgaggt cgtactggag gtttgtgtac
     1141 tcaaccaccg acgacgtgat aaccttcgga tcggcgagcc aggtgccgtc ccagtccctg
     1201 aacgatgcct tcacgatctc gacgttctgc ccggtcttgc ccgagtagat ggacgcggcc
     1261 tcgtcggggt gttcgaggtt gtactccgtt gccttgatgt gcgtctttac gacctgcttg
     1321 acgatctcgg ggtgctcacg gatcagcgag ccgctcgcaa cgaggacgca gcatgcgtga
     1381 tctttcagca tctcgcccga ctgcaccacg cttcttcccg taccctccgc cgcgatgatg
     1441 gacggggacg ggtggggcag gaagacgccg tcgacctggc ctgcggagat ggcggtggtg
     1501 gcggcaccgg ggtccatggc gacgatggtg acgcttgcgg ggtcgacgcc gttcttctgc
     1561 agccaggtcc ggaggatggt atcctggatc gttcccggcg ggaaggtggc gatcttctta
     1621 cccacgaggt cggcgggggt cgagtacggg acttcgttcc tgagcaccag gtccgacccc
     1681 tgcgtctgca ccgctgcaac gatctttgcg tcaagcccgg tgcccacggc cgcgatgaac
     1741 ggggcggcgc cgacgtaggc gatatccagg tccccggcaa gcatcgcctg catctcaggc
     1801 gcgccggtgc cgaactggta gtcggtcacc tgggtgacac cgaggggtgc aaggtcttcc
     1861 tgccaccatc ccttctccat cgcggtgaag tgggcggtct ggtgggtgct cggctggtag
     1921 ccgatgcgca ggttcgttac gtcgccggta tctgttccgg tgcacccggc gacgaacgtg
     1981 aatgccgcga ccagggccac ggccagaaga attgcggtta atgatctcat gggtctctcc
     2041 atccgtatgg gtaacgatct attcttcacg ctttcggtat atagtattgt cgaaaatctg
     2101 agatattgtt atactttggt agcaattttt gccttgattt ttgccttata ggggtgatcg
     2161 ggtgctggac ggggcgcacc ttcaggggtg acatacgcgg acgtcaggcg acggtgatcg
     2221 ggcggcggcc gtacgatgtg gtctgaggta cgttgttctg aagtgcgact gcccgccgga
     2281 cgggaacatg agcgcccccg tgcccgtact cctccgcccc ccgggcgtgg ttttaatctc
     2341 tccagcggtc aaacggtaca gacaggactg ttgataggat gcgaacggat cagattcgac
     2401 agggtcgtct ctcgggagaa cgttcgggcg atctcgagca ctttcttgca tcgatggacg
     2461 ccgatcgctg gattgcaaga gcggaccttc tcgtggatat ggcgcacctc ctcggccttc
     2521 gccggcagga gatcatcgac gaagctccgg cacgggcgct gatgacggtg ctcctcgacc
     2581 tccacgacca cggcctcccg gaggaggcgt tcgacgagca cttcgaggac gtccatgcag
     2641 ggaaggaggc ctacctcatc gaccgggtcg gcgaggattt cggcggccgc ctccacatgg
     2701 gccggtcccg gaacgacgag gttgccacct gcatcaggat acggttgaga gaggagatca
     2761 tcgccctcgt gcggtcgctc accgacctca gggcgaccct catcgacact gccgccggac
     2821 atacggagac ggtgatgccg ggcttcaccc acctccagca cgcccagccc acgacgctgg
     2881 cccactacct cctcgcctac gagcaggcct tctcccgcga tgcggcacgg ctgcgggagg
     2941 cgtatgcccg ggtggacgta tcgcccctcg gtgcggcggc gttcgcctcg accggttttc
     3001 ccctcgaccg cacctacacc gccggcctcc tcgggttcgc ccgcccggcg gcaaacagca
     3061 tggacgcggt cgccgcccgg gacttcgcca tcgaggtgct tgctgacgca actgtctgca
     3121 tgacgacggc aagccggctc tgcgaggagc tggtcctctg gagcaccgcg ttcttcgggt
     3181 ttgtccagct cgacgacgcc tactgctcct catcctcgat catgccccag aagaagaacc
     3241 cggacgtcgc cgagatcatg cgggcgaagg ccgggtcggc cgccggggaa ctcgccgcgg
     3301 ccgtcacgat cgtgaagggc cttcccatga gttacaaccg cgacctccag gaactgaccc
     3361 cgcacctctg gcggggcgtg gaggcagccc ggcagagcat cccgcttctt gccggcatga
     3421 tcgggtctgc ggccttcaac accgaacgga tggctgccga ggcgggcagg ggcttttcta
     3481 ctgctacgga gctcgccgac gtcctcgtcc gggattacgg acttcccttc cgcaccgccc
     3541 accggatcgt cggacgggcg gtacggcacg gttcgctcga cctcgccacg ctcgaggccg
     3601 ccgcccggga agcggccggc ctctcggtcg tggagctcgg gataacccag gagcggatcg
     3661 actctgtcct cgacccgcgc cacgctgttg ccgtgcggag catcatcggc ggcccggcac
     3721 ccgcggcggt tgccgttcag atcgcagatc agaaggaact cctcgcccgc gacgttgcat
     3781 gggcggagga gacccgggcg gcgctctcga gcgcgctaga acatcttatc attgaatcac
     3841 ggaggctggc agcataacgg agatacttca gaccggagac cgggtgcggt acgcgaacgg
     3901 cgggaccgcg ctcaccggca cctacatcac aaaacgcgac gggatggccg tcgtcaaact
     3961 ggacagcggc tacaacatcg ggacgtcccc cgaaaagatc gaactggtcg ggcgtccggc
     4021 cccgcagccg ccggcaggtg ccggggtcgt cgtccagaac tccgacctcc cggacctcgc
     4081 catcatatcc accggcggga cgatcgcaag cagggtggac taccggaccg gggcggtgat
     4141 gagccagttc tcggcgaacg acatcctgcg ggcgatcccg gaactcgggg agatcgcccg
     4201 ctaccgcgac cgccaggtcg caagcatcct ctccgagaac atgcacccgg cactctggca
     4261 ggaactcgcc cgggcagttt acgacgagat ccggcacggc gtatccgggg tgatcgtcac
     4321 ccacggcacc gacacgatgg cctactcggc ggcggcgctc gggttcatgc tcaagacgcc
     4381 ggtgccggtc gtctgtgtcg ggtcgcagcg gtccgccgac cgcccgagct ccgacaacgc
     4441 catgaacgcc ctctgcagcg ccgccgtcgc aaccggcgac ctcggtgaag tggcggtggt
     4501 gatgcacgcg acgaccaacg acgaccggtg cgccatccac cgggcgacga gggtccggaa
     4561 gatgcacacc tcccggcgcg atgccttcca gagcatgggc atggcgccgc tcggctacgt
     4621 cgactacccc gcgctctccg tcaccctctc ggacgaggcg gtccggcggg gaacggggga
     4681 gccggagctc cgtgatgcgc tcgaagaaca ctgcgctctc ctccacttct accccgggat
     4741 gccttcctgc gtcctcgacg cctttgctgg ctacgcgggc ctggtcatat cggggacggg
     4801 gctcgggcac gtggcgacgg agtggatccc gaggatccgg gagatggtcg cggacgggac
     4861 gaccatcgtc atgacgtcgc agtgcctgca cggccgggtc tgcgaccggg tctacaacac
     4921 cggaagggat ctcctctcgg ccggcgtcat cgagggcgag gatatgctcc ccgaagcggc
     4981 gctcgtgaaa ctgatgtggg tgctcggcaa cgagccggac cccgaacggg tgagggtgct
     5041 gatgcagacc gaccttgcgg gcgagatccg gcggaggtcg gtataatgaa ttatcaagaa
     5101 ctcggcctca aagccgggat cgagatccac cagcagctcg acaccgccga gaaactcttc
     5161 tgccggtgcc ccacccttct tcgggatacc gccgagcgga gcggggagtt tcaccgctac
     5221 ctccgggcga cggagagcga actcggggag atcgaccggg cggcacggga ggagatgcgg
     5281 ctcgtccgga agttctgcta ctacacatac gatacggtct gcctggtgga gcacgacgag
     5341 gagcccccga ctccgatgaa ccccgaagcg ctcgaggtct gcctcacgat agcaaagatg
     5401 ctcggcatga cccccgtcga gcaggttcac acgatgagga aactcgtcat cgacggctcg
     5461 aacaccagcg ggttccagcg gacggcgctc gtcgccctct ccggctccct ccccgacggc
     5521 tgccttatcg agacgatctg cctcgaggag gaggcggcgc agcgggtgga ggacgagatc
     5581 ttctccctcg accgcctggg gatcccgctc gtcgagatca ccaccgcccc ctgcatgcac
     5641 acccccgagg ccgtccagca ggtcgccggg cacatcggca tggtgcttcg ctccaccggc
     5701 agggtgaagc ggggtctcgg gacgatccgg caggacgtca acgtctccat cgccggcggc
     5761 gcccgggtgg agatcaaggg cgtccaggaa ctcgacctca tcgccgaggt ggtcaggcgc
     5821 gaggtcgagc ggcaggttgc cctcctcgcc gtccgcgacg ccctccggga gcgcggcgcc
     5881 cgggtggacc acaccgtcgt cgacgtcacc gccctcttct ccgggacggc gtccgccatc
     5941 ttaaagaagg caaaatccat cctggccgtc cggctttatg ggtttgcagg cctcgtcggc
     6001 cgggagatcc agccggggcg gcgtctcggg agcgagatgt cggactacgc gaagaagtgc
     6061 ggcgtcggcg ggatcttcca caccgacgag ctccccgcct acggcgtcac cgcagatgag
     6121 gtggcctgcc tgcgagagtt cgtcggcgcc ggagagaacg acgcggtcgt catcgtggcg
     6181 gcaacccggc agcgcgccgg ctgtgcggcc gagcaggtga tgatccgcgc ggagatggcg
     6241 ctctccggcg tgcccgagga gacccggaag atgctcgagg aagggagcac ggcctacatg
     6301 cgcccgctcc cgggagctgc ccggatgtat cccgagaccg acgtctttga ggtcgagatc
     6361 ggcgccgccc ggtgggagag gatcggggtg cccgaactcc ttaccgaccg ggcggagcgg
     6421 ttcgtccggg agttcggcct tgatgaggcg ctggcacggc aggtggcgtt ctccgagcgg
     6481 ctgccgacgt tcgagaaggc cgtcgccgcc ggcgtccgcc ccaccaccgc ggcccggacg
     6541 ctccttgcca cctgccggga gctcgcccgc gacggcgtgg ccgtcggccg ggtgagcgag
     6601 gacgagctcc tcgccctcct ctccgcggta gaggccggcc gggctgcaaa ggaggcgatc
     6661 ccggacctta tcgccgaact cgcacggacg gccggcgcgg gtgacggggc gcctctggac
     6721 cgggtggatg cggccatcga gaagatggcc cccgccgtct cggaggcgga tgtggaggcg
     6781 atcgtgcatc gtatcgtggc cgagcgggag gcgttcgccc gggagaaggg catgggcgcg
     6841 ctcggccccc tgatggggat cgtcatgcag gagatccggg ggagcgtgga cggcaaggtc
     6901 gtcagcgaga ccctccggcg cgagctcaag cggctgcttg cctgatatcc ggcacgaccg
     6961 cccccggcgg ccgtgaaacg tgcccgaacc ggccccgtcg tggtctatcc acaacggggc
     7021 tccgggttac ttttatcacc gggtctgctc ataaatataa aggagttcta tcatgggaaa
     7081 gacaggaaca gttcagtggg cccaggttaa gggcgtcaag gggcagataa gactcgtccc
     7141 tgcaagtgaa gccgaagtga agaaacccgg cccgaaccag cggttcaagc ctgcggcgga
     7201 tatcctcaag cgggcgaaga aagaggggca ggacccccgt cgcggcggtc gcggcggtcg
     7261 tggcggtcgc ggcggtcgcg gcggcagagg cggcggcacc cccaccatgg acgtgcgggt
     7321 acgccggagc gtgcggcgcg ccaaggtgtc ggctctcgga accaagcaga aatcgagata
     7381 agccgtctcc ctcttcttct tcaatacttt ttatactagc gagttccagt atgtggtatg
     7441 gatcttaaaa ccgccatcag aacctatcag tttgccgaac gggcgaaatc cgaactgatt
     7501 gtcgggtcgc agctaacgac agctctgatc cagttctcgc tccaggagaa gcccggcgga
     7561 aagcggatgc ttgtgatggt gctcgagtcg atccgctcgg aactcgagtt tgcctatggc
     7621 gatacggaac tcatagagtt caagaaggcc atcgatcacc tcaacgaggc gatcagcctg
     7681 accgagagta tggaactggg cgccgcgtcg gagcggatga gcagagccgt cagcgccgtc
     7741 acgaccgcag cccaggcagc atgggatacg ctcaaagagc atgaactcct ctgagtttct
     7801 tcggttcgtg aaagcacggt cgtcggtccg ggaatactcc ggggagccgc ttgatgcgga
     7861 ggatgtcgac tacatcctga cctgcgcgag tactgcgccg agcgccggca accgtgaggc
     7921 ctgggacgtc gtcgtcgtca ccgatgaaga cgtgaaagtg gaacttgcgc tcgcggccct
     7981 cgagcaggtg catatccggg aggcgccagc gatctttgtg gtctgtgcga actacgtcag
     8041 gtccatgtcg cagtacaagg agcgggggat cctctatgcg ctggaggacg ccaccatcgc
     8101 ctgtacctat atgatgctcg ccgcacatgc ccggtccctc cactcgtgct ggaccggggc
     8161 gtttgacgaa gatgaggtcc gcgaggtcct tggcctcccg cagcatctcc gccccgtcgc
     8221 gctcctcgcg gtcggcaggg ggacgccgcc gctcgaaccc atgaagcgga tgccggtgga
     8281 agagcacgtg catcgcgaga cctggtaagc tatcaattct ggagaaatta tgccggatta
     8341 tatggttacg cttgaatcgg cgtggataat taaagacgtc aagtctatgg acgacgcggt
     8401 gagcattgcg atcagcgagg ccgggaaacg gctcaacccc tcggcgaagt tcgtggagat
     8461 cgaggccggc atgatcgcct gcccctactg tgagggtgaa ttgaacacgg ccctcgtggt
     8521 cgccggtacc gctctcgtcg ggctcgtgct ccagatgaag gtcttccgtg cggagtccgc
     8581 cgaacatgcg acccggatcg cgaagtcggt cgtcgggaag gcgttgcgcg acgtcgcgct
     8641 gaaggtccag gacgtgcagg agatatgatc gccgtcgtcg ggcataccgc catcgatcac
     8701 ctcttccggg ttccgaagct ccccgggcgg cacaactcga cgtacatcac cgatcacaag
     8761 gtctacttcg gtggcggggc ggcgaacatc gcggccggga ttgcggtgct cggagagcgg
     8821 tgccggctga tctcatcggt cggcggggat ttcccgggca gcgactacga cctctggatg
     8881 cacgatcttg agatcgtgca ggacttctcc gtcgtgaagg atgcccgcac cgcgactgcg
     8941 tatgtcttta ccgacgaggc cggcgaccag gagactttct tcgagtgggg cgcgtccgcc
     9001 gcgttctccc gggcggaggc ccccgcactc gaattcgtcc acatggcgac ggccgacccc
     9061 gacttcaacg tccgggtggc cgagaagagc aaattcgcct ccttcgaccc ggggcaggac
     9121 ctcctccgct ataccccgga gcagcttgag atcatcctcg ccaacatcga catcctcttc
     9181 tcgaacaacc atgagatgga ccggatgtgc gagatgctcg ggaccggacg ggaggcgctc
     9241 gtcgcgtcgg tcccgatgac ggtcacgacc cggggagccg aagggagcat cctctgcatg
     9301 gacggcgagg accaccatgt cccggccgtc accgtcgcgg ccgtcgaccc caccggcgcc
     9361 ggcgacggct accgggccgg gttcctcacc gcgctccgga gaggctacgc cccggtcgac
     9421 tgctgtcgcg cgggagccgt ggtctcgtcc ttcgtcgtgg agaggacggg cacccagaca
     9481 aaccttcctg actgggaccg gatgctcgtg cggtatcgga aggtctttgg cgaacccgga
     9541 gaaataattg tctgaattat ggggaataca gcgctgatcg ccggcaggaa ggtgccggtt
     9601 tctgcccccg gcccgatatc ggcatccggg ggcgcctgat ggagtccggc cccgacgtcc
     9661 gtgtcgaggg attgacccgg tatatcttct ccgcaccgtc ctggccgcgg tcgcttgcga
     9721 tcctcatcgt gctcgggctc gtcatcgacg ccgctacata ccgggccggc aacgaactct
     9781 ttctcgtcgg caccctcggg ttcacgatcc cggcgctggt cgccttcctg ctgaccgtgc
     9841 cgctggtgag ggtctctggc aagcagatca cccataaccg gtcggctctt cttgccctgg
     9901 cctgcacggt cattacggtg atcctgagcc tctcgccggt ccttgtcttc gggctggagc
     9961 tctttccgac gctctatgcg ggcgctctcg gcccgatctt cggcctccgg ctgctggttc
    10021 tcgtagccgt cgccgactac cggatctccc ggatggtctt ccccgcgttt gtccagagcg
    10081 cggtcgggat agcggtcggt gcctggttct tcacgcccgg gttcgccccc tacgccctcc
    10141 tcctgcaggt ggtctttggg ctgggtttcg tctctctgat ctggctgatc gagcggccgc
    10201 tgaagcgggc gttccagatc agcgggctca acttcctcaa caccttcatc gcccacctga
    10261 ccgacggttc gaagaagatg gaggacttct tccgcgagat
//
LOCUS       DAJB01000049            9873 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_75040, whole genome shotgun sequence.
ACCESSION   DAJB01000049 DAJB01000000
VERSION     DAJB01000049.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_75040"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1742 a         2735 c         3235 g         2161 t
ORIGIN      
        1 ggccgaactt tttagactta agagtcgact gccggctctt ttttgctgta gccatactat
       61 caagtaagga gatatataca aataaagata actctcatcg ccaggatatg tagatctctt
      121 ttttccaatc ggggctcaaa aaccatccct ctcctgcgcc atagagcgac gatctcaccg
      181 gccccggtgt gttccatcgt cggaaaaata gggggtgctt aaatcgcgtt ggtactcgat
      241 gatttggggc cggattactc caaccactca tcgtcatcat actcctcggg atcgtctgcc
      301 gtggagcgga gcgaccacgc gacagactcc gtgaatttaa acgtcacata agtccgttca
      361 aattccgaaa accccgggaa ggttgcgatg gcgtggaggg cctcggcaac cttgccccgc
      421 ccctcagtca gtctttttgt aaccccctcc agggcccgca tcttttcagg ggtgatccct
      481 agtgcctcct ccactgtatc gcaggaccgg atatagccgg ataacgacac ggcctcagag
      541 taacaagctt cttgctccat catctcctct cacctcttct tccgtgccgg tttgcgcttc
      601 cgggtgccgc tggccttcgc ccggggtttc ggcgtgtcgg gactgatgcc aaacatggat
      661 tccaggtcat gtaccgggtt ctcgatgagc caatcaacct gatctgcacc tcgccggccc
      721 cgctttttct tcttcttctt gtcctcctcc tgatccagcc gccgacccct ccggctctgg
      781 tcccgggtgc ggggcaactg agtggtcgcg aagaactcgg acggtaactc gatgttgcgg
      841 ccggggtatt cagaggggaa ctctgtctca ttcgcggtgc tcggcgtggt gctggtgccc
      901 gcccccatct gcacccccgt ccgcgtccgg gtcctcgtac ctactcctgt gccggtcccg
      961 gtcccggttc gagtccttgc acccgtcccc gcccgggcgt cgggcaggga gagcgggtca
     1021 acggcgaagt ccgggatgct gtctggacgg tccccgaggc gcagccgggc accgggagtg
     1081 aaccgaggcg agacgctcgc ccggggaggg gagatggcgc tcccgaacgt gagatccaga
     1141 tcccgggcgg cgtccctgct cagagtgtcc gcccaggtgt cgaggtccgg catctcccgg
     1201 gccggggcga gggtcggacg ctcgtagccg aagatgtagc ggggccggtc gtctacgacg
     1261 ttgcgggccg gagtgcgggc tggcgtcctg gtgggggtgc gagatatggc gctccctgcg
     1321 gtgaggtcgc cggggaagga ggacccttca cgaggcatga gccgctcggg gcttgaccac
     1381 cggggctcga tcatgctgtc gatctccggg gttcgcaccg tgcggccgcc cgtgggcagt
     1441 tcggggagcc ggaggatacc ctcgcccggc tggatctccg gggttcgcac cgtgcgaccg
     1501 ccggagatga gctccgggcc ggcgtagtag ggagtcggac ctttgcgccg aaccggatcc
     1561 tctcgccggg acaattcgcc acgatggtgg agatagtcct cgaagtcctc gccggagtac
     1621 cgctctcccc gatcggtgtc gaggtccggt atatctagct ccggtgtgcc gtaccctgca
     1681 ccgccgtgag ggtcagacca ctcgttcatc cagtccggca tccccgtggg catacccgcg
     1741 gccgtcttgg tttcgggccg gctccagtgc atagcgggga ggagggttgc ccctgcaccg
     1801 gcgatcttca tcacatcctc gatcgcggtc tttgccgggg tcacataccc cgccccaccg
     1861 cctccggtgc ggatcacgtt cggagctgtg cgtccggcgg cgagccggcc ggcagccaga
     1921 gcgccgcccc gggcgagacc gccccctacg gcacctgccg ggagggcgat gcttgcaccg
     1981 atgagagctc ccttcaccac gtcttccgga tgccgggcga agtgctcctc gtaggacggg
     2041 acggcaggga tgacggcccg ggctggagca gcgaccagat ccacctttct cccgacctgc
     2101 tggccgacga aggaggatcc gaaccgcgcc tgtggcgacg cgatgaagtc ccggccgctc
     2161 tggactagcg ggccggtccc ccgcacaacg gcatcagacg cctgggccat gacggcccgg
     2221 cgctgctccg cacgttccat ctcagccgct tcagtctttg cagcatggtc ttttcccgct
     2281 gcggcaatag ttcttgtccc tgccgctatg ggttggatta ccggacgagc cacgacagag
     2341 gttacccggc cggcagtatc tacggtgcgg gtggccgatc ggatccctgc cgttataggt
     2401 tggatcaccg gacgggccac gacagaggtt acccgtgcag ctgttctcgc tgttctatca
     2461 acggcactct ggacgtttcg cgcctgctgc tgaaaccagt tccataccat gttagtccct
     2521 ctcgtagatg ttggcgaact tatcgtactt ctgccgcttg aacacggtga tgaagaagtt
     2581 cccaatgccg atcagcggga tcaggtacat gatgaagaag tagatgttca ccaggaggtt
     2641 gacccggccg agggagtccc actccgccgg cacatcgaag acgccggcca gggtgaaccc
     2701 gtcgatcatg gtatcgatga cgaccccgcc ggcaaatgac atcaggaccc cgatcacgat
     2761 cgagatgacc atgatgatga atccctgcca tagcgcgctg aaactcgtca tactctgtcc
     2821 tcctcaagtg cccgaacgta ggaccagatg aagatcccga gcaagccgat gaccgggata
     2881 gcgccgaacc atgcgagcga ccagttcatc gccccggctg tctggacgcc gacctgcccg
     2941 tccccgatca tcccgttggc cccctcgatc atgccgttga tcatcggcgt gaaacagatg
     3001 tagagcacgg ccacgccgat gatgaagact atgactgcaa tgaggccgta tgcaatccct
     3061 tcgtcatttt tcatcatgat tctgcctctg ttccccggcc ggggggaggg aagaaatcaa
     3121 ttaccctgta caaaatatat gatttgtatt tggataatgt cgagttgctg tacctcaata
     3181 ttcctttaat ccatgccatt ccagcacccc ctcgtctctc cagtaccgcc accgtgacgg
     3241 agatatgccg gcgtccttta atgcctcccg gtgggacagc ccttgtaggc ggtactgctc
     3301 gtaggtaggg atgagcttct ttgcggccgc atacatctgc tttgtcttca ggcttggttt
     3361 cttcccggga agggatcctt cctcctcgtc ctcctccccc tggttcgccc gggccgcgat
     3421 gattcttcgg gtgacctcgc gccgtaactg atcatactcc ttcgccagga acttaggcgg
     3481 ccggtgaatg acgtatttca cgatcttctc cccgtctgcg gcgacgaggt gcggagtgat
     3541 ctgttgattg ccgagcctga agagagtctt cgtcttgaac gccttcatca gggagatacc
     3601 ttgcccatac accccgcgat ccatctctgc catgtaatta cagagcatcc tcggcacttt
     3661 gtcgaggagg aactggtttg gcatggagag aatctgtaca gtttgcgaaa ccctattgat
     3721 ttggaaaata tcgttcttat cccggttctc cttcgatgcg aacgcccggc tgttccagcc
     3781 gacgccgata tcgtcaaaga tgtagatgtt gtggggtttc gcgttgccga tgatctcaga
     3841 tgctcgttcg gggtcgatga ttgcgacatt atccatagag aaatacttcg gccactcgcc
     3901 cccatcacca tcaaggattt ctgccaccag ccgggctgtg ttgtaggcga tccgcaggga
     3961 ggcataggat ttcccgctgc ccttcggccc ctggagaagc atggagatat cctgtttgta
     4021 ggggccggtg atggtatgag cgaagcgctg cgcgaggtgg tcggtatacg ccgccagcgc
     4081 catcccgttc cctcgggcgg gttgctgctg ctcagacctc cacggtgata tcaccctcgt
     4141 cgctgttgcc gacatctgca tcaccccctc cgccgaactt tgcctcatcg tcctcttcgc
     4201 cgggcataat tccgcagacg ccatacgtca tctcggttgt catgccgact acccgatcga
     4261 tccagctcca caggtctccg gcgacccggg tacaggcgtc gagcattgcg gttgccctgt
     4321 ctacggggga ggggtctttg tactctccca gggcttccct gtaatactcc ttcttcttcg
     4381 cccggatccc ttcgcgtgtt tcggcatctg tgacccccga aatccactga tccatgtaag
     4441 gggatatccg gaggtctctg acatccgtca tctgagcgat gagcgtcccg aaccctgcac
     4501 cagtgagaaa gaaccagtga gtatactcgt ctttggcgct catcggcgga gtgccgagcc
     4561 ctactttcgc cacgagatca cctctttccg ggcgaggacc ttctgccacc gggcgaacag
     4621 ggccagcccg gcatatgcct tcgcggtgga gatcgccgtg tcccgggggt cgagccaccg
     4681 gacgatctcc tttgcgagtt ccgcgccgtc ggggttctca gtgtcatcct catcgaggat
     4741 ctcccggtag ggagtggtga gcgtgaataa gcaggagagg cactcataga agtcgaggta
     4801 gggccgctgg agcggccccc ggggatccat gagatagtgt ttcagggcgg tctcctgccg
     4861 gacacaggcc aggtatgcgc cgtcgatcat ccgcgctatc gcggggcggt cgttgccggg
     4921 ggtggggagt gattcgccca cgacatcacc ctatgatggt tgtcgcaacc gcaccggcaa
     4981 tgatgatgcc gatgatcacg atccctgccg tcagcaggag gccgtatttc ttcatcatat
     5041 cctccatctg ctttctgacc ttgcgctcga tgaggagacc gtactggtgg aggtcctgcg
     5101 agaggtaggc agtggggata ttcttgaagg cgtaggcgta ggagaacatc ccgctgctcg
     5161 ggatctcaac cctcgcggtc aggtcttgcg cctcaacgat gaggtcggcc agggcgttgg
     5221 ggctagtgac gttctgccgg atctcgtcgg ttgcggtcat ctcataaaga gactcgcagt
     5281 actttcccag gtcgtcgcgg ggggtggcga gaagggcgtt tacctggtcg ttggtgagga
     5341 agttgagttc ggggaactcg gtcctcacgg tggagagcac cgcgtcgatc gccatgcagt
     5401 gccgggcgtc gagggcgagc gggagcgtgg tcccgaagtg gtagactttg aggccgtttg
     5461 ccatccggtc ggggacgatc gccccggaga acttcgggtc tatctggatg ccgaactctt
     5521 tggtatcgaa gatcggatcg cctttctcgt ccttttggcc gacagaccag tctgtttccc
     5581 cggagccggg gacggtgact gagataatgg ggaggttctt tttccgggca gtggagatga
     5641 tccgccattc ggggacgtat cgccggacct ccgagacgac gaggatcaga ccgatgtatc
     5701 caccggcggc gatgagggcg attgcgatta agggtagggt atccaatacg attgccatta
     5761 gttttcccct ccgaggaggc ggccgatgat gccgaggccg cgttttgatt ttttcttctc
     5821 ttcgttgtcg gtgatggtcg agatctgctc gtaggagctc cggttctgga ggatacgctc
     5881 ccgttcgggt ccctgtgccc gggtgccgac aaacctgttg aaatgtgcac ggaccatctc
     5941 gatcaggtgg gggtattccg ggaggttaat atcgactctc gtgcagctga gggttaagag
     6001 gttgagcgcg atgtcgaggt cgatctctgc cagttcaggg tccttctggt gagataggag
     6061 ggccgtgtgg ctgaagtagt tttgaatcgt tgtgatgaaa ccccgttttg ctgccgggga
     6121 gaggtgggac cggagtacct tgtcggtgag gtatgggcga taatcagaag agtagagcga
     6181 ttcgagatag ttgtagtgct cggctatgta cgggtgcgac gggcggccga ttgccgggac
     6241 ggggcgctta gtgggttctg ctccggcgcc ttcgtcagat ccgtcatatt cgtcgtagtc
     6301 gccatagtcg tcaggccccg ggtaagggga gggttcgaac gagtccatgt ctcatgtcct
     6361 tgttggtcat gctgctgcct gaaggagagg ggggattaga tcccgccccc attgctgctg
     6421 ccgccacgga ggcgcatgat tgcccagatg gcaacgctga ggagggagat cagaccgacg
     6481 aggatgatga ggccgctgaa ctgcgtccag atgcccgctc cggtcttgag gtcggtgttt
     6541 tcggtcgcgt tccaggccga ccccgcaggg atggtcgtag aagcgtcaat gttgctcccg
     6601 atcacaggtg ccagagagaa cacggcaacc aggacgataa cggagattgc taccccaacg
     6661 aggtagtcca tcgtgctagc ttcggcacga tcattgagga taaggttcat ctgcttttct
     6721 tttgcccacg ccttcgcggc gttgaggcgg gccagaacgg tatcggttcg actcaaagtt
     6781 ctttcgattt cctccgatag ggtattgttt atgcagttat cgtatataaa agtatggggt
     6841 ttcgggtcat ttctgccgcc ggataatagc aaatacggcg atgagcccga tgactaccag
     6901 gacaatgggg agcgagccca tgagcgtgag tgaggtagct gatccggaca cggtggcatt
     6961 atattcttcc tcatagggcg atccggtcat gttcgctgcg ctatccgctg ccccgagtgc
     7021 cccgtacccg taggtgaggc cgatcaccag ggcgaggatg acgagggcga tactgacgat
     7081 gggtacaaat tttgtcattg tttacgtctc ctgtaagtag tccaggccga ggttcaccac
     7141 gagcgcgaac gccagaatcg ttgcgacggc cgcgaccccg gagaggagga ggccgaacga
     7201 gggatcgatg gtggtgacgg gctggagggc gtactccgcg atcgtgatac tcgtggtgtt
     7261 ggcatcggtc aggacggtta ggttctcggt ggtgagcgcc gggaggggga tctggtctcc
     7321 gacgttgccc gccagggtgg cgagggcgag gtaccaggag aggaatgcag ctccgaccgc
     7381 cgcgaggatg tcggcgtaga gccgggactc cccgccgaag agcgccacga tgatgagggc
     7441 ggcgtcgagg aggaggaggg cgatgaagat ggaggttgct atcattgccc tgcctcccgg
     7501 atcaggatct cggtcatgcc gtcgccccgg gcctggagga tctggttcat ctccccgatg
     7561 gcccgatcga ctttggtgtt cctgatttcg tagactttcc cttcgtcgtc atagagcgtc
     7621 aggtgcccga aatagagtcg cggtgtcata gtaatcatgt cctctctttg atgatgcccc
     7681 acacgatcgc caggatgctg agcgcgatga acgccacggc ggcgaggtga tactctgcgg
     7741 gggttttcgc cagcataatc ccgcccagga tgatcccgat cgccgccggt atcgccatgc
     7801 tgccctgccg gagccattgc atgaagaagg ggatggcaaa gacgatgacg tagaagaacg
     7861 agccgagggc ggcggtataa ggttgctcta cggcggcctg gatgccgagc cagtcaacgc
     7921 cttcggtgga ctcattgagt tggtcgtcgc cgccgaacgc cccgatgagg gcggagtagg
     7981 gggcttcgtc gagggtcggg atggtggatg tggttttcgg tcggagcgtt gtatcggcgc
     8041 tggccggggc gacgaggagc gccgccgcga ggcagagggt tagcaggatt gtgagtgttt
     8101 gtgtgcgttt catgtcattc actccctttc aacggccggc cggtgaagaa ctggaccacg
     8161 gcggcgacgt agacgaacca gatcggtccc tggaacatca tggcgacgat cggatctatg
     8221 ccgtaactgg tgaggagcgg gatgatcgtc agggcggttt tcaatccctc gaagaagacg
     8281 ccgccgaggg tgaatagcac ggtgatcggg tagaggggcg atgccccgcc cgatgtggcc
     8341 ccggcggtga tctccgtgac ctgggattcc ccgaacgcca tgtcgctccc agggagcgcg
     8401 acggtgaaga tgccgagcgc gttgacagct ccgacgacaa agccgaaggc gaagagggcc
     8461 agtgcgatgg tatatgctct gctcattggt atccttcctc ccggtaccgg gtgacgatta
     8521 tccccatcac cgcgagggtg ctgacgaggc ccaggcatat cgggccggtg aggccgaggt
     8581 cgtcgagcca gccgaaggcg tagaagagcc agccggcgag agagatcacg accatgcccc
     8641 gggtggcgct ggtcgcgccg aagatgccgc cgatcaggaa gaggaggagc ccggcgacgt
     8701 agatatacat gccgtccggg agcgggccga gcggcacccg gatccctttg aacgcgacgg
     8761 cgaagtcacg gtggacggtg ccgaagtcag ggtggttcgc ttcgatgcga acgaagtagg
     8821 agtgcccttt gtagttggag aggtcaaagg tatgggtgac gttggcgtcg tttgctacgc
     8881 tgtgcgaggc gacgacctcc tccggggcgg aggtgttggt ggtgttctgt tgcgagacca
     8941 cgatactgag gctggtggtc ttcttggtcg tgtcgttgta gtcgatatgg atatggccgg
     9001 tgtcgtcttc ggtgctggtg gtgacactga tgttgatcgc tctgagagcg tcgccggcag
     9061 cggggaggag ggggccgttg gtggtggaga tgatgatcgg gtaggtgtcc tctttggggt
     9121 agatgtcgag ggtttgattc acatcggggg gcttgtagag gatgatatgg tacttgacgg
     9181 cctcgatcat catgaagtta atttccccgt tgctgtcggt ggtgccgttc atcagctctg
     9241 tggtgatcgg ggttgatgtg gtgttgatgc cgagcagact ttcgaggatg ttgggggagg
     9301 gggtctgctg gatcccctgt gcggagacgt tgacatcggg gatcggggca ccgagtatag
     9361 tgaggattgt gaatttgacg ttgtgcggcg ggtagttggg gtattccggg gcttgggttg
     9421 gttctgggcg gggggtcagg aggaccgttg ccgtggtggt gccgggccgg tcgatgtatt
     9481 ggtattggct ggttggatag tgcccttcgg cagctgcggt tatggtgtag gggccgtaat
     9541 cgacggtgaa cgtcgcgggg tttgtggtgc tctcctggcc gtcgctgagg gagatggtgg
     9601 cgttctggac gtttgcgagc gtgtcgccat ccttgacggt gacggtgagg aggtaatgtc
     9661 cggtgagggc aagattgtgt tgggtggcgg tggcgtccac agtgacgggc tcattgaggg
     9721 cgtcgtggag cgaggcggag gcggagaggg tgtatgaacc gggtgtgacg ttgtggaaga
     9781 ggtagaagcc cgtcgaggaa gtggttgtgc tgccgtagcc gtcgagggag acggtcgcgc
     9841 cgctgatcgg ctgctggctg ccctgggtct gga
//
LOCUS       DAJB01000050            2793 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_174772, whole genome shotgun sequence.
ACCESSION   DAJB01000050 DAJB01000000
VERSION     DAJB01000050.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_174772"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          600 a          733 c          779 g          679 t
ORIGIN      
        1 gaaattaacc tgacgccgat ggaagctgat agatgagata tcatggctca tgatgtgcgc
       61 cctgttcacg gtatcccaat ttatttgatc gccaaattgt tctccaaatt tcctcatatc
      121 accaggaagg aaattactgt taacctttgt caaaggcaat gggtttctta caagccatga
      181 tctaaaggca atatagcgga acataggctc atcatcgctc ctgcctttaa tcgtttctgt
      241 gaggattgga atgaggtttg gatgtaaggg tacatagtgt tcatatcgga ttttatcgag
      301 tgatggggga acaagaatca ctggatgctc tttactcaag gcatctctaa acataccgac
      361 cgttagtttt tcaattgttg cctctgcgcg ctgtgcagta tagcaaccaa accctaccaa
      421 agcaagatag ttgatgcaat tggatttctt gatcttacct gcgcgatagt cattcactat
      481 atgggtgcag aggttggcta catcatcaag agcaatgata cggtccgtaa ccaccttacg
      541 ctctttaatt gcttttggtt tctctaagaa catttcaaat gcttggtaat tcgcattcag
      601 atacaactta ctcagatgct tcaggaatgt tctgccaaaa cagatttgtt tgttgatgtt
      661 gctgttactg cgggaatttg tcaagatgtg attttttaat ctggttgttg tctcatggtt
      721 gataacccct cctgtttgtt cccatactac tcgcgcgctt tttctgatcc agtaaagtga
      781 tttggggctt aaaccccgct ctctttcttt ggcatactcc atcagttctg catgtgtaaa
      841 ttggagtgtg atttggccta aatttgaccc tctttcttga tttacgcttc tcttgtcagg
      901 tatggcgctt agctgaatgc cnnactctta ctaatgcgaa actcttctcg atgaaacgtg
      961 ttttatcagt tctcagtctt ttcctcccgg tttcagttcg atctcatcaa acgtcacgac
     1021 gtgtgagtcg aagatctcgt cccggtcgtt cacggagagc tggacgaaga tctcggcggg
     1081 tgcgttctcc atagatatga cgtccccatg ctccgccttg acggcaaaac cgaggaactc
     1141 cagttcgctc cggacggcat ccctggtgtg gtaggtcgag agtatatccc tgaccttcgc
     1201 gaggacgttc tgctgctcct cctccctggc catactcctc tcatgcaccc tgtggtatat
     1261 taacctctcc aggaagggta cccgacgggg ctgcgcaacc cccggtcact cacggcactg
     1321 gccccggggc agatgcaggc aaacgtataa gtaaaaaaat tacctatagt aataagatgg
     1381 agatacccac cccggctgaa ctgcgggaaa aaaggctccg tatgggctta aagcaggcag
     1441 aagtagcccg tatggcggga atcagccaat ccatggtcgc gcggatcgag gcaggcagtg
     1501 tggacccgag ggtgagcacg cttgccagga tcgtggaagt tctgcgggca gcggagcact
     1561 cggcgatcac ggcggccaat gtgatgaacg cacccgtgct ctccgtcgct ccggacgacc
     1621 ccgtcagccg tgccgtcgag atcatgggcc agaacggcat ctcccagctg ccggtgctcg
     1681 agaacagggt gcctgtcggg tgcatatccg aatcggcgat catgaatgcc atggaagaag
     1741 gggggcttca ccagacgcac cggagactgg tgcgggacta tatggagccc gggttcccga
     1801 cggttcctcc gacggcgccc atcgatacgg tcgtccacct cctgcaccac agccacgcaa
     1861 tcgtcgtgct cgagaaggga gaggtgcagg gtgtcatcac caagcacgac ctgatatcgt
     1921 tgatcacgta ggctcttcta gatcagagag accaggtccc ggaggactgc ttcgggatcg
     1981 tcggccttga ccacgctcga cgcgaggagg acgccgcagg tcccgaggtc gacggcaatc
     2041 tttacgcatt cgcccgactg gatgcctgct ccggtcagga ctctgacgtc cgggttgacc
     2101 gccaaaacag cgttgacgga ccgctcgatg atccccgggt cggctttcga gaccgagacc
     2161 ccgctcccga tcagttccgg gggctcgatc gccacgtagt cgggccggag cgctgctgcg
     2221 gcggcgctgg tggagtcgtt gttcgtgcag acaaccgact cgaggtgaag ccttcgagca
     2281 gcctgaacac aggcgtcgat ctcggccagg gtgaggcggc gttcggagtg gttgatcagg
     2341 gtgccgcgtg cgcccgccga cctgaccgct tccgggagaa tatggccggt atgggcaccg
     2401 ggggcgatcg catcgatatg ctgggcgtag atgggtatcg catagtgatg gctcatcggg
     2461 tggatctcgg tgaagaccgg cgcgatgccg atgacggcgc cgctctcttt tgccacggtc
     2521 tcggccgcag cggcgatccg gtgagcgttg ctgcccatac cttcctgata ggtcttgaga
     2581 ttgaccagaa tgaacggtga aaccatattc atcacttctg ccaataggtt gctcctcctg
     2641 atattaactc tggtgccggg tgcgggcact gggagggcgg atcgcctggt gggtgagcgc
     2701 ggggtggcca cgacttatat gcagcggagt tcgaaaactt cgatgagtgt aaagcgcgac
     2761 taagtgaagg gcgtcaacta cccccggtta aaa
//
LOCUS       DAJB01000051            2510 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_31677, whole genome shotgun sequence.
ACCESSION   DAJB01000051 DAJB01000000
VERSION     DAJB01000051.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_31677"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          419 a          873 c          815 g          403 t
ORIGIN      
        1 aggaatacgg caccgacggc gccgaagatt gggcccggga ggttcagcgg aaaggccagg
       61 gcgccgaaga ggtcgccgac gaggaagata acggagatca ggatgagcag cccgaggttg
      121 gcgttgagga acgcgacaat ctggaggaag acgggggtct ggacgtaggc gccggccagg
      181 atgtttaaga tgccgagcag gatcaggaag atgacgagcc cgacgattct tgcaatgagg
      241 acgccgacga ccgattcctt tctgcgctca cccatgctat ctctcgttat ggtgttttcg
      301 ttccccgttc cgtcacccgc gtctgcgcct gctcgcggac gaatgagagg aggtagtacg
      361 gccccccggc acccctgccg gtcgtcccgc tcgccttcca gccgccgaag ggctgggcgc
      421 cgggccaggc ccccgtcgtc gctcccccgc gccggttggc gtagacgacc ccgaaccgga
      481 tcccatcgaa gaagtacctg acctcctccg ggtcttctgc aaatatcccg gcggtcaggc
      541 cgtagtccgt cgcgttcgcg agcctgagcg cctcttcaag ggtctcgaag gtgccgacga
      601 ggaggagcgg gacgaagagc tcttcgcgga agaggcggtg gtcgtgcgca agatcggcga
      661 tgaccgtggg cctgacgtag acgccccgcc cgtagaaccc ctcctcggtc accgccccgc
      721 cggtaacgag cctccccccg tccctgagcg cctcctcgac cgcttcctgg aaggtcagcc
      781 gtgcgtcgtc gtcgatcagg gggccgtacg cgacctcccg ccgccggggg tcgccgacct
      841 ccatccggtc cgcccggtcg cggagcgcct gcaagaaccg ctctgcaacc gacgaatgga
      901 cgtagacccg ggacgtggcg ctgcacttct ggcctgcaaa cccgaaggct gcccgggcca
      961 ctccctcggc cgctttcgcg atatctgcgc tggcggtgac gatcgtcggg ttcttgctcc
     1021 cgagctccgc caccagcggc ttatgatagg gctgcaccgc ggcaacctgc cggtgcagcc
     1081 acatccccac ggcccgcgac ccggtgaacg cgatcccggc aacgtcgggg tgggctgcca
     1141 cgacctcgcc gaaggggccg ccgggcccgg tcaccaggtt gagcgcaccg ggagggaccc
     1201 cgttctccgt aaagagccgg tagaggttca tcccggagag cggcgtcttg ctcgtcggtt
     1261 tgaagacgac cgtgttcccg gtgaggagcg ctccggcgat catgcccgcg gcaagcgata
     1321 tcgggaagtt gaacggggat atgacggccc agacgccgta cggccgcata acgctccggc
     1381 actcctcccc gggtgccggt gagtgcatgg gaaagatgta gccgttgctc tgctcgtaga
     1441 tatcgcagta gtagcggagc atagcggccg cctcgccgac ctccgccagc gcctcgaagc
     1501 gggtcttccc cgcttcaaag gtcagcagcg cggcgagtgg gaagagccgt ttctcgatcg
     1561 catcggccgt cttcctgacg atctcgatcc gctcccgcca gtcccgccgg ctccacccca
     1621 aaaatgcctc gtttgcggcg gcaatcccct cccgggcatg gtcttctccc ccttcgggga
     1681 aggtcccgag gaggatgccc gggtcgatgg gcgatcgcgc ctcgaactcc cggagggcgg
     1741 tgacctcccg gccgccgatg tacatcgggt ggcgctcacc gaactcctgc ccgatctcga
     1801 gcagggcggc ctcgtattcg ccgtgaatac gctcgtccga ctcgagcgag acatacgtaa
     1861 cttttcctga ctcctgcata ctatcaacct ccgttaacat cctccagtgc ccggtcgagg
     1921 attgcaacgc ctgcatcgat atcctcctcc tcgatgacca ggggcgggct cgcccggatc
     1981 accgactccc ccgcggggag gaggacgaga ccgagctcga acgcacgccg gaggactttc
     2041 ccccgctcgc ccggcgccgg ctccttcgtg gaccggtccc ggacgagttc catcccgacc
     2101 atgagcccga gaccgcggac gtcgccgatc atcgggtggc gctccgcgag ttcgccgagg
     2161 cgcctcacca ggtaatcgcc cagggccgtc acccggtccc cgaacccctc ctcgcgcaga
     2221 aggtccatca cggcgagcga tgcggcgcag gcaaggttgt tgccgccgaa ggtgctcgcg
     2281 tgcgacccgg gtggccagtc catgacctca cttgatgcga cggtcacccc gaacggcagc
     2341 cctccgccga ttgctttgcc gaggcagacg atgtcgggga ccacgccggt gtgtgtggag
     2401 gcgaggaacc gcccggtccg gaagcacccc gactgcacct cgtccaccac gaggaggatc
     2461 ccgtgctcgt cgcagagttg ccgcaaccgg gggaggaagt ccggcggcgg
//
LOCUS       DAJB01000052            7225 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_133808, whole genome shotgun sequence.
ACCESSION   DAJB01000052 DAJB01000000
VERSION     DAJB01000052.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_133808"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1270 a         2272 c         2227 g         1456 t
ORIGIN      
        1 ggatctccga ccggtgtggg aaaaaatgaa gagttatctg atgatgaaat ccatcgagaa
       61 ggtggtctcg tttccggttg cttcttccca ggggcgcctg tatatcgcgg taaactcggc
      121 ggaaccgggc tctgctgcga catactgcca ctggtgcaca ccaccggcgc ccacgacctc
      181 tgtctctggt gcgatgaagg tctcgttcac gtattgaagg ccggcagatg aggtgacgtt
      241 ccacctgtag ccggtcgtcg ggttctcggc gaggctgatg gtgatggcgc tcccggccgg
      301 gagcgtgacg gtttcgttgt tgttcgtctc gttaaaggcg aactcttccg ccgggatggg
      361 cgtggtggcg accggggtag ccgtctctgc cggggtgggc gtctcggtct cgttccccgg
      421 ttcctctggc tgcgatatac aaccagcacc aagcatgcac aataccagga ctcctgcaat
      481 aaggattccg tagatgcttt ttacctgaac catgcggtaa gattgctcat gatcggtaaa
      541 aatctttcta aaagcctcca gtccgacgac aggcgtctga ccgggtctta ttccgatatg
      601 aaagccttcc ccgaaattga tcgttccggc gctcttcgtg ccactcgggg tctatctctg
      661 tatgacggtg atctgttccg tcgaattcac cggagcctcc ccctgaagtt acccccgagg
      721 tcacgacggc tcctgccacc agcacaaccg tctccggggc tgctcaggga aacgtaaccg
      781 cggtttcctt ccctatgcac cggcaggctg gactgaagac gagcctgccg gagcttcctg
      841 gactgtcgag gacgggcagt ggacctgggc gtcccgtgac ggcaagggcg atgcccgggc
      901 atcgatcatg atccgggatt cggcttgtct cacagcgtga gctaccggga gtcgcgtgac
      961 tcgctcgtcg cgttcgagac gaccgaaggc tactacaggc aggctctggt cagcggcgac
     1021 ccctcccccc cgggagtact tcaccaaacc tgcgtcctac ggcacctggg tcggcgtgaa
     1081 tgaacggttc atggtctgtc agtgttgagg acgaatccag gcaaaatttc gacaacgttc
     1141 gtaaacgtcg tcgattacgg caactttgcg aacctgaggt gacccaaaaa actctttctt
     1201 ccgggcaagg gcctatcgct ccggcacggc agtgatgatg taatgatacg gccctgattt
     1261 cgtgacagtc gtaacccgga gccccgcctc cgccatcaac gacgccgcat actccgtgcc
     1321 gaacttcttt tgggccggcg ggccgagagg gagaggttct ttcttccagt ccacgtcgac
     1381 gatccgcccc tccggggccg ccatccgttt tgcgttcatg agcatccgga tcgggtcggc
     1441 aaaatcatgg agagtgatgc cgaagaagac gatatccgca cagccatcac agaggacggt
     1501 ctcctcggcc gtcccccggt ggaggacgac gttctcgagc cccccggaac gggccttctc
     1561 ctgcagcaga tcgagagcct cggcgtcgat atcgatcccg cagacacggc cgctcggccc
     1621 gaccagccgc gccgcaggaa gcgcgaagaa cccgtccccg cacccgacgt cgatgaatgt
     1681 ctcccccggc cgaaggccga cccctgaagg atagactcgg ggttctgcca gagccggcgg
     1741 gcggcctcat cctcgggcga gtgtctccag gaatgcctat gatgcaccat gccaaccccg
     1801 gtggcgtgca tcccggaaaa aatctgctgc tccccgccag cgggtctctg gatgaccccg
     1861 gtgaggccgt cgaaaaagaa gtttccggga ccctgtcgcc cctcacttca ccgccttgcg
     1921 aagtttctcc tgcacttccg gctgctccgg gttcatctgg tgggcggtct ggacatgccc
     1981 ctcgacacgc ttcatcaact cgttctcgct ctctgccttc tcctcaaact cgcacgcaag
     2041 gccaaggtcc ttgcacctga atgtcctgac ttcctgcata gctctcaccg gcaggagttg
     2101 tatatcgggt gagatataaa aatttcgtcg tcttttttct gctgcaaccg cctcgctgag
     2161 agccggaggc gcgattgcag cctgttgctt cgggaacact atgggacagc acttcgtaac
     2221 gttcgacggg ccggggagcc gggattcacg cctcttcgcc cgggtgcggc cgtttcctgc
     2281 actccgtcac atcttcaacc agcccgtccc gcagggtgac gaccctgcag aagtactcct
     2341 tatgccaatc ctcgtgcgag accatcagga tcgtctggcc caggtcctcg ttcaagcggg
     2401 cgaagagatc gaggatgctc cttgaggtct ggctgtccag gttggcgcag ggctcgtcgg
     2461 cgagcaggat gctcgggttg ttcacgagcg cccgcgctat cgccacgcgc tgctgctccc
     2521 cgcccgagag ctcgctctgc cggtggttca tccggtcgcc gaggccgacc cgggaaagga
     2581 tctcggtact cttcctgagg tactcctcct ctggatcccc acgaacgagc gatgtcaggt
     2641 agacgttctc gagtgccgtg agctccccga tgagggcgta gtcctggaag acgtagccga
     2701 gccggttcag ccggaagagg gtccgctggt ggtcggagag gccgaggaca tcggtcccgt
     2761 cgatcgtgac cctgccggag tcggggcggt cgaggagccc gaggatgtgg aggagcgtcg
     2821 atttgccgct gccgctcgcc cccatgatcc cgacgaactc tccccttgcg actcgaagtg
     2881 agacgtttcc aagggcgcgg acctccaccc tgcccatagt gtagacccgg gtgaggttct
     2941 ctgcgacgat cactcgttca ccccctgatt gcggagagga tatcctcccg ggcgatccgc
     3001 cacgccggga tgtagcccgc caccaccgag accataaaga ggcttgcgat gctccggagg
     3061 atcgccgacg cctccacgag cgggtagacc gggccgccgg ggaagacgag tgggtaggcg
     3121 gtcaggtagg tcgtcacgcc ggcggcgagg gctatcccgg ccagcgcccc gacggcggtg
     3181 atgaagagca cctggaggac gtaggagttg atgatgatct gctggtcgat gccgatcgcc
     3241 ttcaagatcc cgatctgccg ccgccggttc acggtgttga tgaagatcac gatgaagact
     3301 actacgacgg cgatgatcag gctgacgagc gtcgagatgg cgttgatgat attgaagctc
     3361 tggatcgcct ggttgacgaa ccccccagcc ttctcctgga atgtccggac ttcttcctgg
     3421 atgccgtggg agagaagttc ctttttatat tcctcctccc tgccgttcac ctcggtcttc
     3481 accaggatca cggatgcctg gtcgcggagc ccgagcaccc cgtccatctc gcttctggtg
     3541 acgaaggctg ccgtatcgac gccgaaagac tgggtctcaa agatcccctt cacccggtag
     3601 gtccgcatct cgccgttcgg gtaggccacc tccaccgtgt cgccgaccct gaccccctgg
     3661 agcgagggga gaccgcctgc ctcctccccc tcttcccccg caagggtcgc gcccatgatg
     3721 atcgcatcgg tatccccgtc cgagagaaac tctccatcgg tcatgtactc cgcgattctc
     3781 gtcaccgacc gctcgtcctc cggggttatg ccgtagagcg tgcttgcggt gttcctgccc
     3841 ttgtaaaagt aggtcacccc ggcggtcgtc ctcggggacg tgccggtgat tcccggcacc
     3901 cgttcgacct tcttctggag accggtcgcg tcatcgatgt accggctgcc ctcccggggc
     3961 tcgatgacca ggtttccgaa gtcgaagtcg atcgactggg cgttaaaggt ctcaacgacc
     4021 ccggagatga tggaggggag gaagactaag ttcacgaaga tgagagcgat gatcatgatg
     4081 gtgagggcga gcgtcccctt gctcccgcgc tggatggcct tccacgccag gaacgcggag
     4141 acccggaggc ctgcaaacac gccggtcact cctcctttcg tctccggtac cagaaggccg
     4201 cggctacgac ggcgacgatg aggacggctg ccgcggcggc gaagaacggg agtgtgttcg
     4261 actctgcaac aacaacctgc agggcctgcc gggttgtgtg cgccccgtag tcgtcggtat
     4321 actgtatggt gagggtgtac gggaacgctc cctcccggtc ggcctgcagg gcgaagacag
     4381 ccggaccgtc gttgccgggt tcaatcgtcc cgaggaacgc ttcttttgaa cccgggagag
     4441 agagaccctc gatggttgcc cggaccgacc ccgcgtctgc cgtcccggta ttctctatcc
     4501 ggatcgtcag gtccacctgg tccccggcgg tgatccggag ggctcggtcc tgatggaggc
     4561 gatgcccatc tcggcgcggc cgacgatggg aatgccgatc gtcgccacct gctgaaaggc
     4621 tgtccggtcg gcgttccggt agtcgacggt gacgaggacc ggtgtaagtc cgagcggtgc
     4681 gttggtgtcg gtctcgaagg tcaggttcag caccgactcc ccccccggtc ggatctcggc
     4741 gatgtaatag ttctccgagt tcctcggggc gatcgcgccg gaggtcgtgt tgacgttgac
     4801 ggagacgtcg tttgcactcg cctggccctc gttgtagatg gtgagcgtga cgttgaaggg
     4861 gtcgcccggg tcgatggatg ccgggacggt cctttccacc cggagcgcgg gtttcttgat
     4921 cagggcgtat gccgagttga cgttcaccgg tatgggatac cgtacgcctg ccgcgcccct
     4981 cacccggacc cagacctccg ggaagtagat cccctcctct ccgggggcgc gtatccggaa
     5041 ggtgagcggg aaggactggc cggggccgat ctccccgata tcctggaaac tcccagtcag
     5101 gacttcgact ccttcgccgt tcacgagcac gttctcgatg taggcgttct ccggtgtctc
     5161 cgttgaggtg ttcatccctc cggtgcctcc atccagccgg atctcaagcg gcgatcccgt
     5221 ggccggggga gcggtactcg agagcaccac cgtgatcgtc ccttcgttgt tgggcatcag
     5281 gacggcaggg gttacggtgt agttggagac gatcactgtc ggggcctgtg cggttgctgt
     5341 tgccggaatg agcaccaaaa ctacgatgag cacacacggt atccagccga tcgtgcgcct
     5401 ccccatgccc ctccccgggg cagccgcgga ctccgggacc atctctctcc tctccaggta
     5461 tggttggagc tcccgaatcg ctgatgttca tatatatctg ttgtgcatga ccgctccctg
     5521 ccggctccgg ggttatgcac gataggtcta tgactcgaaa atatttttat ggttctagta
     5581 gtataatacg ttcgagatac ggagaggaag gtgcaggatg ggttgacccg gtgggcaacc
     5641 gcaaacgacg tgtgcttcca atcacgttct atacctccct atatgtcatg ataaccccgg
     5701 tatgcccata ccgccgtgct gtctctccgt tctcatgcac cgattctccc acaccacgtt
     5761 tgcccggcct gtcgaccgct tgcaggggaa ccggcacggg agcatggcga tctcccgaga
     5821 agagggttga cgaagagttc tcccgaccgt gagctgcttt tatcttcccc ggccggtata
     5881 ctccgcccac gtctggagga gaagagatgg agacgaccca gtggctggtg acgctgacgt
     5941 tccgggtgac ggtgaacggc gtcatgtcga gggatgattc ggtagctgca ggcctggaac
     6001 aactcaagat ggggctctgc agcggagagg atttcccggt ggaggcgagt gtccggagga
     6061 tcacgaacat cgtcgaggag gagccggtct tcggcgtgaa gtggtgagtg cctgggccgg
     6121 tacgagccgg aggcgttatg gtgagactgc cgattcccgg gggaaaactc ttttctatcc
     6181 ccgccgcctg atcggcccca tgcacgaact tcccgataca gttgccgtcg atgtcgtcct
     6241 tttgccgccc gggccgatca tggatatgac gatcagcgca aaccggaccc tgctcgccgg
     6301 caaccccgac ggagggatcc gcctcgacag agagaactgc ctcccgcaca tctcggtcgc
     6361 gatgctcccg gtccggcgtg aggatattcc ggggatcgtc gcgaaggtgg gccggattgc
     6421 cggacagtgc tcccccatgg ccatgaccgt cgatgccgtc gcaaaacacc ggagcagcac
     6481 gggagagacc gtctccgttc tccacatcct ccgaccggag atcctccagc tcttccacaa
     6541 gaccgtgatg aacgcagtaa agccctattc ggcacccgcg gccgggccgg agatcttctt
     6601 cgaggaggtg agcggaccgt ccgtcgactg cctcctccgg ttccagaaga cctccgtgta
     6661 tgagcgctac tccccgcata tcacgctcgg gttcggcgac ctcccggagt tgatccccgg
     6721 gattgacttc ccggtccggt tcgaggtgac gaggggcgcc gtctgtcacc tgggcgttca
     6781 ctgcacctgc cggcaggtcc tcgccggatt cgatctcggg acccgggcgc caaccgcccg
     6841 ccgcaggacg gcccgccgct aggccggcgg gctacagcag gagccccttc acgtactgcg
     6901 ttgtgcagca ggcgcacccg acgtaacggt cgagcgtccc ggtctcgata acctcgacct
     6961 ccgggggctc ttcccgcatc cggcgggcga atcccgggtc ccgttctgcc atcatctgca
     7021 gaaacatcgc gagcggcatg aacggccggg cctgcacggg gacgatccgg aagtccgtaa
     7081 catccgtatc gagcgtcgag ttgcgggcga ggtcttcatc gaagtagagg atgacctcta
     7141 acccgtggcg ttcctgcagg tccgtcaggc agccgagcgg tccgagttcc tccggttctc
     7201 cccggggaga agactgcatc cgatg
//
LOCUS       DAJB01000053            9702 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_67565, whole genome shotgun sequence.
ACCESSION   DAJB01000053 DAJB01000000
VERSION     DAJB01000053.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_67565"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1910 a         3097 c         2954 g         1739 t
ORIGIN      
        1 aaaccggggg tctccttgcc cgtcgcatgt gaatactgca aaactcctcg aactcccaat
       61 ggattaaaga accgacccga cataccaatc agacacctat ggcaccaata cagcacggcg
      121 acactctgct cctgcatttc accagcaccc gccccgacgg cgaggtcttc gaggataccc
      181 gaacgggaga accggtccgg gtaacccttg gcaaggacca gatcaatccc ctcgttgaga
      241 gagcgctgat cggcagaaac cggggcgaga cggtgaccgt ggtgctgccc ccggagaagg
      301 cctacgggag ctatcgcaaa caactcgtga tcacgataaa acgcaagaaa ctggagctcg
      361 accacgaacc ggtccccgga gagttcatcg gggtcgaggt gatgggaaaa ccctgcctgg
      421 tgaccgtcgt cgacaaaacc gacaagaccg tcacggtcga tgcaaaccac ccgcttgcag
      481 gcgagacggt cacctatgcg atcacggtcg tagcaatact tccccggagc ggatagcccg
      541 cgtgcgcgaa ggggtcgctc acccggtcgg tgagcccgtc ccccgcgtga tgaacgcaag
      601 gccgggagat agagttcgcc gcaccggccc gggcgttatg ccctggcggc cggctcgaca
      661 ccccgctccc tcatttccgt gcgcatccgt tccggctggt atttatgccg tccgatgaga
      721 gctctcttaa tggcatccgg ttcatccaca agcgagagat cgccccggca gcaggcgatc
      781 gctggatttt ttgtactcct gatcatcatt ctcgccccca tcgcactgtc atactacctg
      841 caccctgcac cgtctccggc aacagaaccg gtgcacgtgc agatcctcgc ggtcaacgac
      901 ttccacgggc aactgaccga cgggcagaac ctcaacaaag aaccggccgg tagcgcaccg
      961 gtgcttgcat cctacctgga aaccgcgata gcggatgccg gtgaggccac cacgttcatc
     1021 gcgctccccg gggacgtcgt tggggcgtcg ccgccggaat ccgggctgct gctggacgaa
     1081 cccgccctgc tgttcttcaa cggtctcgca gacgactgcc cggacataat cgcgaccttt
     1141 ggcaaccacg agttcgaccg gggcacggac gaactgctgc ggatggtccg cggcgggaac
     1201 ggtgcgacga ccatcaccca cctggcagac ccctacccgg gtgccgaagc ggagtacatc
     1261 tcctcgaacg tggtctggaa gacgaacggc acccttcttt ccgcccccta cacgatccgc
     1321 gacgtcgacg gtgtaaagat cgcatttatc ggtgccgata cgacaaaaac gccctcgatc
     1381 cagaaggcgg tcaatatcga actggttgcc ttcgaaaacg agaccgaatc gatcaaccgg
     1441 tatatcccgg agatccagca gaagggagtg cacgcgatcg tcgtcctcct ccacgaggga
     1501 ggctcgcagg acgcctacga cggtccgacc cgggaaggtg gcaacgtgac gggcagggtc
     1561 gccgggatcg tcgccggcct tgatgcggac gtggacgtcg tcctctcggg ccatacccat
     1621 gcgttcacca acgcctacct ggagaacgcc ggcaaaaacc cggtgctggt gacgcaggcg
     1681 tacagttaca gcagggcctt tgccgacgtc gatctcgtca tcgatcccgt caccggtgag
     1741 atcacccata aatcggcacg gatcgtcccg gcatacagcg accggccgcc cggcaccagt
     1801 cccgacccgg aagcctctct gctgcttgga atgagcgaag aggcggtcgg gccgctgacg
     1861 aaccgggttg tcgcgaccgc atcggcggat atcacgcgtg aagagaccgg tgccggcgaa
     1921 tccgcactcg gcaaccttgt cgccgacggc cagcgggcag cgatgaacgc cgatatcgcg
     1981 ttcatcacca ccggctcgct ccgggcggag atagcagggg gcaacgtcac ctggggcgac
     2041 ctgtatgcgg tgcagccgtt ctctgcgacc gtgctctcga tgaccctgac gggagaccag
     2101 gtcaggggcg tgctggaaca gcagtgggag accccgttgc cgccccacaa cctcgccgcc
     2161 tccgggctta tctacacctt caacgatgaa aggcccgccg gcagcaaagt taccgagatc
     2221 cgggtgaacg gcgtcccact ggacccgaac gcggagtaca cggcggcaat ggtcgattac
     2281 ctcgcaaccg gcggcgacgg gtataccgtg ttcacgaacg gaacgaacgt ggtcaacggc
     2341 ccgttcgacg tcgatgcact ggtcacgtac atggaatctc ttccggagcc ggtggacgcg
     2401 aaggcggagg ggaggatcac gatgatcgtg tgaaggggct gtccgttcac gcggccggcc
     2461 caacttataa ataacgcccc gccgcatgag cccccggagg tcatctgaga tgcctgaatt
     2521 tgcacaaccg ttctccggga accacatgga acgtaaactg gaccggggag aactcatccg
     2581 aacgatccgg ttctcgatcg ccgccgagta cgaggcgatc cagctctaca accagatcgc
     2641 cgagtcgacc gacgacccgc tggtccagaa agtgatgcgc gatatcgcgg acgaggagaa
     2701 ggagcatgcc ggggagttcc tgtgtctcct gcgggagatc gagcctggcg aggaggagtt
     2761 ctaccagcac ggctacgaag aagtcgagga gatgatcgag gagttgaaga aaggcgggaa
     2821 gtgatctccc ttccatggcg gggattcgtg aaggtttgcc ggcgatcaaa tccggcgccg
     2881 gaaaccccgc ccgtcagagc cgtttgtcac gacgctttcc taaaaagcat acactcaaat
     2941 acgacttcgg gtgaaacttc agcgatggag actcgcaccg gaacgatcgc ggagagggtg
     3001 gcgcagaaat gcgagatgct gatcgaccgg gtggagttta tcgatctgca cttctccgaa
     3061 gagattctca ccgacaggat actcaggaag gcaatgtaca aggagttcca ggaagcggtc
     3121 gaagccgcgg ccgacatctg tgccctcatg aggcgcagtc tggactcttc ggcgcaggac
     3181 gactacagca acgtcgacta cctgatcgaa cgggagatcg tcccgccaga tctcggccaa
     3241 tccctgaagg aggcaaacgg gctccggaac aggctggtcc atgaatacga cggcatcaac
     3301 gaccgaatag cgtatgcctc aattacacgg ctcaccgaag ccctgcgcga attttccggg
     3361 gtgatactca cgtggctcta gaggtaaccg atacagcact cctcgaacat ttccgcgagg
     3421 tgctgccgtc catcaggggt atcctcgtct acgggtcgca tgtcaaaggc tacgcagacg
     3481 aacgctcaga catcgacatc tgcctcatcc tgcacgagag cgccgaccgc gctccggtct
     3541 acaggcggat gctcacggcg ggagagcgct atgatatcgt catcttcgac gagatcccct
     3601 ggtacctcag gggcgcggtt cttgaggcgc atgcggtgct ctacgccgac gatccggacg
     3661 atctggacta ctggctctat cgacaacttg ctgtctggcg cgacatgaag cgccggcaga
     3721 tgccggtctc atccggggat ctgctccgcc gcgtccggtc gtgaccgcct gctcaaaaga
     3781 agtgttcccg ccgtcgcaaa ggttccgggt cctgtttcga ccgttcccgg agatcacctg
     3841 gagcgcaaaa tcgtttgccg gcacggcagg taatcgagaa ggagttctac cagcacggct
     3901 acgaagaagt cgaggagttg aaggccggga agtgatctct ccttcccgtc acctgatcct
     3961 gcaggactcc tcgtaggatt tcctgcagcc cgggcagagc gtccgggcct tctcgaggtc
     4021 cgggtagttc tcttcggtga tctcgtcgca ggccgccccg cagatctcgc accagaactc
     4081 cttcgtctcc actttctctt cgccttttat gctgcgtttt gtcatgcgat cgtcctcccg
     4141 ggtacccggg tgagtcactg ctctccttca tcgggcatat gctttcgcgt caccactgcc
     4201 cgatcccggt aagatcgagc cccatctccc agaaccgatc ctggaccttc gggttgagga
     4261 cgagggacga cggttgggtc caccgggtct cctcgaagta gccgccgttc accttcccgg
     4321 catccggcga gacggcgagg tgcaccacca cctccgcccc ctgttccggc ggggcgcctt
     4381 catccctgcc gtggatatag gcccggagga gcttcgtgtc gatcaccccc gggtggaggc
     4441 tgttcgccgt cacccccgtc ccctcgaggg cccgggcgag ccgggcggtg aaggcgatgt
     4501 tccctacctt cgatacggcg taggcatcgt aggggtcgta gtacgaaaag cccgggaggt
     4561 tcccccagtc gatcgacccc acgctccggt gggcgatcga cgcgacgttc acgatccgcg
     4621 agggtgtgct ccgctcaaga agccccagga gttcgtgggt gaggaggaac ccggcgagga
     4681 agttcacggc gaacgtcatc tcgatcccgc cgggtgcaac ctcccgctcc ggcatgaaga
     4741 ccccggcgtt gttgacgagc acgtcgagcc ggtcgcacgc ctcgccgact tccctcgcga
     4801 ggtcccggac ctgctcctgc accgagagat cggcaatgaa gaggctcaat cggtcggaac
     4861 cggtggccgc ctccagttca gcaaggaccc cacggccctt ctttcggtcc ctgccgtgaa
     4921 gcagcacgcg atggccctgc ctggagagga tgtgtgcggt cgccttcccg atgccgtccg
     4981 tcgagcccgt aacgaggatg gtctgctccg tcatatgcgg cccggatata tgccgtctct
     5041 acttgaacgt ttgtgttcaa acccggtgcg ctcctctctt ttgacaggca gaccggatcg
     5101 cctccggggt caaatgcacc atggcgacgg ctgtttttgc ggtcctggtg cgctacagac
     5161 caaaaaaatg atattatgca ccacaaaatg ctattatggt gcgatactcg gtcacgatgc
     5221 atgacgacct cgtgaagacg atcgacagga tctgcgacac ccgggggatc tcccggtccg
     5281 aatggctcaa cgagctctgc acgacgcact tcgatggtgc accgggtaac ggcgtgcagc
     5341 ctgtggtccc cgcaggggtg ctgcctgcaa gcgttccgga gtcgaatatg accgattacg
     5401 atgccgcctg cgccaacttc acgatcgacg tccccgacca cttcaacttc gggtacgatg
     5461 tgatcgaccg gtgggccgag accgaccgga acaaactggc gatgatctgg gtggaccagg
     5521 cgggaaacga gaagaaatac accttccgcg acctgaggta tctctccaac ggtgccgcaa
     5581 acatcctctt gaaatacggc atcaagaagg gcgaccgggt catgctgatg cttccgaggg
     5641 tcccggaatg gtggatcttc gtcgtcgcgc tcatcaaact cggtgcggtc ttctgcccct
     5701 gcccgacgat gctgaccccg aaggatatca aataccgtgt ccaggccggc aagttcagga
     5761 tggtcatcac caactgcgag aacgccgaga aggtcgatga ggtcgccaac gcctgcccgc
     5821 tgctcgatac cctcttcctg gtggacgggg agcggaaggg ctgggtgagc tacccgcacg
     5881 aggtcgaata ccctgcaccg gtatcgcacc acaaggtcaa catgccggtc gcgaagaagg
     5941 cgaaatcgac cgatccgatg atgatctact tcacctcggg caccaccggc gaggccaaga
     6001 tggtgctgca cgaccacagc tatccgctgg ggcatatcat caccgcctcg ctctggcagg
     6061 acgtcacggc aaccgacctt cacctgacct tctccgatac cgggtgggcg aagtgcgcct
     6121 ggggcaagat cttcggtcag tggatcgccg gggcctgcat cttcgtctac gacatccggg
     6181 ggaagttcgg cgcgaccgag ctcctcccgc tgatcgagaa gtacgaggtg accacgttct
     6241 gcgcaccgcc gacggtctac cggatgctga tcctcgccga cctcgacaaa ttcgacttcc
     6301 gggatctccg gcactgctgc agcgccggcg aaccgctcaa ccccgaggtg atccgtgtct
     6361 ggcaggacgg caccgggcag cctatctacg agggttacgg ccagacggag accgtctgct
     6421 gcatcgccac gttcccctgc atgaagcata agcccggttc catgggaaga cccgctccgg
     6481 gctggcacgt cgaactccac gacgacgacg gaaaggcggt cggccgcggc gaagaagggc
     6541 gcatcgccat cagcctcaac ccccggccgg tcgggctctt cgtggagtac ctggacaacc
     6601 ccgaagcgaa ccgggaatcg ttccagaacg ggttctacta caccggcgac aaggcacgta
     6661 tggatgagga cggctacttc tggttcatcg ggcgcagcga cgacgtcatc aagtcctccg
     6721 ggtaccggat cgggccgttc gaggtggaga gcgccctcat ggagcacccg gccgtccagg
     6781 agtcggcggt cgtcgggtcg ccggacctca tccgcggcat ggtcgtcaag gcgttcatcg
     6841 tgctgaagtc cggctaccag ccctcagaat cgctggtcaa agaactccag gcccatgtcc
     6901 ggaacaccac cgccccgtac aagtatccga ggtcgatcga gtttgtcgcg gaactcccaa
     6961 agaccatctc cggaaagatc cagaggaaca tcctccggga ccaggaactg cgcaggcaca
     7021 ccaacgggaa atgaacttcc atcgctcaca aaattgaggc acggctttcc cttggatatc
     7081 gccatgactg cccccgcccc ccggggcggg ggaggaggcc ggnnccctcc ccgtcagccc
     7141 caccccgcgt ggcgatagcc accacggtcc agtgcatggt gtttgctcct cgttcgggcc
     7201 gttccgtctg ttacactcca gaaactcgta tcgcacctcg cggcgcctga actctcttga
     7261 ggcgatcctg acagtaaatc tgatgaccgt caaccccgcc gcggcacccg tgcagccaaa
     7321 aaaaagcacc gcccccgtcg ggtgcaaaaa aatccgggac gccccggtca gcagatcagt
     7381 tcgataaaat tctcgaggat cttcagcccg accgcgccgc tcttctcggg gtggaactgg
     7441 accccgtagg tgagcttgct gttgacggcg gaggcgaagg ggcggatgta ggtggtgctc
     7501 gcgagcgtat gctccggtgc ggccgccgcg tagtaggagt ggacgaagta gacgtactcc
     7561 tcctcgcgga gcccctcaaa gaggggggtc ccggggtcgg tgcggatggt gttccacccc
     7621 atatgcggca ctttcatccc gggcacgtgg ggaaaccgct gcaccgaacc gggcacgagg
     7681 ccgagccccc ggtggacgcc gtgctcttcg ctcgagtcca tcagcagctg catcccgagg
     7741 cagatgccga ggagcggcgt attgccggta aggacggtct gcttcaggtc gccgagcatc
     7801 tccattccct cgcggaatgc cccgacgccg gggaggacga tgccgtcggc cgatgcaatc
     7861 acctcggggt ctgcggtgat cgtcggcgct gctcctgccc gctcgagacc ccggaggacg
     7921 ctccgcaggt tcccgaggcc gtaatcaatt ataactatcc tcttcttcat ccgtatctcc
     7981 tcgcttccgc cacaacccgc gctcgatcca gtcgcaggac agcccctgct tttcctgcca
     8041 cccggcgagt tttgcgagcc acatctccca gagttcgggg caggagtcct tgatgatctc
     8101 aaacgttgca aggtcgcttg aggggcacat gaaacacccg atgcggtcga gcccccgctc
     8161 gtagaggggg ttgtagggcg ctttctcgcg gaagatgtag agccagacgt gcatcgccgt
     8221 ccactgctgg atgggagccg ccgagagctg ttgcgggaca tgggcgttcc gccagacccg
     8281 cctgctctgc atccgcttca acgactcgta cttccgctgc ccgataaacg agaggcactc
     8341 tccccactgc tcgccgatca ggcgcccgat ggggtggagc ttacagaccc tgcagcacca
     8401 gcggttgtcg acggcaggcg ggccgttctt ctcgaactcc ttccagaagg tctcctcgtc
     8461 gcagaccctg aggacgtcga gcccgtagag ccccgccacc gcatccacgt tctcgcaggt
     8521 ctccgggaac tcaagccccg tgtcggcaaa gaggagcggc accgggccga gcgcttttaa
     8581 gacgacgagc agggttgcga ggctgtcttt tccgccggag taggagacgg tgggtttctc
     8641 agggttctgc tccgccacct cccggacgaa ccggatgctt gcggcctcgt actccgcaag
     8701 gaccggttcg tttgctcgga cggtatcctc ccacgtcgcc tccccgggga cacagatcga
     8761 agagcagttc ttccgtgtcc ggacgacgag cccgcgctcc atggtcgcgg cggtggcggc
     8821 gtcgaccttt gccctgccga cgccgatgca ctcgccggct ctcgtcagga tgaagacctc
     8881 gtcgccttcg gcgacgtcgg ggtcgatcga gacgaggccg ggagcaagga cgtttgcccc
     8941 gtcgcggatg gatgggatcg cgccgccatt gatgacggcg tagcgtttcg tcggccgcag
     9001 ataggctgcg gccgccgggc gcgggagggg ttcccagcac tgtttatcgg ggaagtagcg
     9061 gattgcgccc acgaccgcgc cgccgaggac gatctcgtcc atgcggtcat cggcggggac
     9121 cttattcagg agcgcgatat gaccttccgg taccagcggc gctccaaagt gctcgctgaa
     9181 tatacggttg atccgctcga tgtcgtcggg gaacgcagga cgtgcgtctc ccggcggcgt
     9241 gacggctacc gggcgtgtcg gggcgccgca ggcgcagacc ccggagagca cgggagcgtg
     9301 gcaggagtcg caccagcgga gcaggatctt tccgaggtag aggcgaggca ttcttatatt
     9361 cgttgggggg aaacggataa aagtcttggt gtattctgta tttttcccgc agtactcccc
     9421 ctgaaatgag cgttttatcc gggcgagagg tcgcccccgt cctgcagcgg catcggcgga
     9481 tgaaaccccg ggtgcgtatt ctcccgggaa cgcgacactc cgggtacagc ggagggcgtg
     9541 atgcgtacca gtgaactacc ccgcccttca gggcggggct tcctgcttca tggacaacac
     9601 ttgcaccaca gagatgtgat ggagaggtct tgactccaca ggctcaaagg gctgttccgt
     9661 ccccacgcgg tggatattta ccgcagcatt gtaatctcta cc
//
LOCUS       DAJB01000054            3212 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_77420, whole genome shotgun sequence.
ACCESSION   DAJB01000054 DAJB01000000
VERSION     DAJB01000054.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_77420"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          640 a         1003 c          967 g          602 t
ORIGIN      
        1 ctcccgcggg gcgaacgtgt acggggcctg atgcgggcaa ttcctccccc ggttaaaacc
       61 gggggtctcc ttgcccgtcg tatatgagag cacttttagg aggataacca tgaagatggg
      121 catgacaggt gagatgaaca ggaatgcgtc gggccgctac gcggccataa acgggctttc
      181 gatgtactac gagatccacg gcaccggccg gccgctggtg ctcctccacg gcgccctcac
      241 gacgatcgag tggacgtttg ggaggatgct gccgtccctg gccggaaccc ggcaggtcat
      301 cgccattgag cagcaggccc acggccacac cgccgatatc aaccgcccgc tgacgtacga
      361 ggggatggca gcggataccg tcgaactcct ccggcagatc ggcatcgagg aggccgacgt
      421 cttcggctac agcatggggc cgccatcgcc ctcgatatcg ccgtccggca ccccgacctc
      481 gtgcacaaac tcgtcctcgc ctcgccggga tacacgagag aaggggccta ccccgagatg
      541 tacgaggggg aagagaacct gaaacccgag gacctgaccg gaaccccttt cgcagaagat
      601 tacgcccgga cggcgccgaa cccggaggac tggcccgtgc tgatagagaa ggtgaagcaa
      661 ctggaccggg agttccgggg ctggccggcg gaggccgtcc ggtcgatccg ggtgcccacg
      721 ctcgttatca tcggcgactc cgacgtcgtc cgccccgaac acgcggtgga gatcttccgg
      781 ctgctcggcg gcggtgtgcc gggggatatc gccgggctcc cggcctcccg gctcgcggtg
      841 cttcccggca cgactcgtgt cacgctcgtg gaccgcaccg actggctgct tttgatggtt
      901 acggaattcc tcgacgcgcc cgtgccggag gccgggggca accgcacgga ggatgacaaa
      961 cctcaccagc gaagaagcac ccctgtagaa tcccaccgag gaaaaaaagg gttacatccc
     1021 cgcccgggag aggatggtat cggccacctg cccgggactc gtgaagggga agtcttccgg
     1081 cgtcaaaagg gtgcccgctt caccggcggt catctccacg tcgcctgccc tgcaggtggt
     1141 cgccgctccc atcgggaatg cggcaaggag ttcttcgggc gtcttgatgg ggaatttcgc
     1201 gccggcgagg gcgccggtga tctgggtacg gatctcttct ttaacgttca ttgttttgcc
     1261 tccgtcattt cagcgatagc cccggcggcc tttgccagcg ggcagtcgct gctgtgttca
     1321 gcacagcaaa aacaggcgct cttcactgca gtgagtgcca gttctgcctt ttcgtccgat
     1381 acggactttc cggtgtaact ccatttcggc atggttctca ccgatcatcc cattggatag
     1441 ttataataat atagatgccc gtcattaaaa tatacaaggt tctcatgcta cacttgcatg
     1501 accgggaaca gcatgatcct gaccggcacc tatcaaacga ggagtttcaa tgactgacgg
     1561 ggcagagcac tacggtcgtg atatcaggga ggtccaatcc gagatcgccg gtctcaggag
     1621 cgaactccgg cggttcatcg agcatgcgaa caggcagcac gtcgatacgg tccttggggg
     1681 actccgcaaa gagtatgcag gggtcttcgt cgagcagcac ctctccgacg cagaccagag
     1741 gctccgggag cggatgatcc gggaatgccc gatgcgcgag aggtgttatg ccgctttcta
     1801 tgagtttctc aagagttctg cagagcacat caaggacggg gaggtgagcg aagaggtcgt
     1861 ccagtcctac cgcgaccggc tcgcggagat gcgcaagggg gggaagttcg acacctgctc
     1921 cacctgcttt accgagacga accgtctctt cgagaaacag atcgaactga tgcgctcact
     1981 cgggatctac cggagcaata aggagacttc ttcatccgtt gcagacctat cagacgagat
     2041 cctggtccgg gatatactcg agccgcttgc aaaccggcac cgcttcagga tcgtccagtc
     2101 gctcgccgcc gaaacgcaga cgttctcggc tctctcgaac ctgaccggcc ttcggggagg
     2161 caacctgctc tttcacctca agaaactcca ggatgccgga atgatcctgc aacggcacga
     2221 gcggggagac tacatcatca ccgagaaggg ttacaggact ctcaagggca tcggcaccct
     2281 ctactccgcc ctgcagccgt agaacctcag gccctctcct ccacgaccct tcgacgcgat
     2341 cgaacgcgcc atactgcccg gcgggagcca tcagaggtcg agagaatatc cccaccgtgg
     2401 gaggactgtt ggaatacacc ctcgccgcca gagcccatgc ccgtgcgccc gtcgctcacg
     2461 cccagaaaat ctatcaatga tcctcgaaca gttcaaccag gaaaggcatt ccgttgagga
     2521 ggttgccggc ctcatctacg ataccgagcc ggtgtatttc tccctcgcta gccctccgaa
     2581 agattgaaaa tcttgtccgg acgggcggca atgccttcgg gcacgagaac atcacctgcg
     2641 ctgtccggga aggcaaagtc gtcggggtcc tgatcatgca gaccgctcac tccccgggac
     2701 tcgggaaaga gttttctgtc atcggccatg cggccggccc cgtcaccgcc gccaggtatc
     2761 ttctcgccga gtggctgatc tttgcaccac actacttgaa gggagccccg gaacccgaat
     2821 actacatctc cagcctctcg gtcatcccgg ccgagagagg acggggggtc ggcacagccc
     2881 tgctccaaga cgtcgtccgg aaggcccggg cctgctcgat caagatcacg ttcggttctt
     2941 tcacgacaat gcgccggttc tccggcatca cgatattgag cggtgcccgt atccgtcggg
     3001 gcgtcggcag cctgcacgat cgtcgccgga accggtatcc tcagggttcc gatgccggca
     3061 cgagccggta gacccgcccc gtctcccggg acggcccctg gttctttgaa gtcaggacgt
     3121 agtgcatctt gtcagtttat gttaggaatt ttggcgtgtg ggggcgggcg ctatggtcgc
     3181 actccggggg tactccggag cacggctttc ca
//
LOCUS       DAJB01000055           11773 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_52299, whole genome shotgun sequence.
ACCESSION   DAJB01000055 DAJB01000000
VERSION     DAJB01000055.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_52299"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2061 a         3759 c         3779 g         2172 t
ORIGIN      
        1 gcgatggcgg tgatcgggtg ctcctgcgcg acggtggcgg tggccgggag gacggcatcg
       61 aagttctcga tgccgtaggc gatcgccacg tcctcaaaga tatcccagtc gtggaggatg
      121 tccgcccggt agcaggggac gaggaccctg acccgggagt ccccgtcggg ctcggcaccg
      181 aaccgcatct tacccaggag cttcgccatc tcctgcgccg agagggagag gccgaggagg
      241 gaggcgcatt cctcgaccga taccacccgt tctgccggcg caagcgtggg catctcctgc
      301 ccgtcgacgg tgaccgtctc gatcgtcgcc ccggcctcgg cgagcgcggt gcagatgatg
      361 ttcaccgcaa tcatcaccgc ttttctgtcg gtgccggtgc agtcgagcag gatgttcttc
      421 gtcgccgtcg tgaccctcgt cagctcgccg ttgatgatgg gcgggaagga gagcacccgg
      481 tcgtcggcat cgacgatcag cgggaaccta tggaagttct ccaccaggcg ggcgtagtcc
      541 cggcccttcg ggtggtcgac gaggatctcc tccatggtca tctcccgctc gaagtccagc
      601 ggcacgaagg accggtcgcg aggggaggcg atgtagcgga agggcggcgt gaccgcatcg
      661 aggtcgtgga cgccgatcgc caccttgccc cgtccgcgcc cgaccgccca gtggagggcc
      721 tcctggaggc tcatcaggct ctcgatcgcc tcttcatcga aactgacgtt ccggatcacc
      781 gccgagccga ggaacggccg gatcccggca aggcccgggt cgatcgtgaa ggtgatgcct
      841 gaagggcgga caggatagac cgggagaccc tcctcgatcc cgaggaaccc gcgcatcgcc
      901 cgggagacac cctcggggga gtagaggtcc ggccggtccg ggaagaactc gacgtcgaca
      961 tggtcttcct cgatccgctc gatgtcggcc ccgatcatcg gaacgcggtc gatgatcgtc
     1021 tgccgatcgg ttccggtcag acgttccagg taccggtagg gtaacgtaat tatcgccatc
     1081 tcatcgcctc ccgccgtagg tgggcgtctc ccggatccac tcgacgtcgc tcttgtagag
     1141 ctgccggagg tcccggagtc cgagccggag catcgccacg cggctgatcc cgaggcccca
     1201 cgcgagcacc gggtgctcga taccgaaggg tgcggtcact tcctgccgga agatgcccgc
     1261 cccgccgagt tccacccacc cgaggccgtc gacatagacc tcgggctcca cggagggctc
     1321 ggtgtagggg aagtagccgg gacggaaccg gaccttctca aagcccatct tggcgtagaa
     1381 ctcctttaag aacccgagaa ggtgacggaa gttgacgcct tcgtccataa cgatgccttc
     1441 gagctgctcg aactccgcaa ggtgcgtggg gtcgatcgcc tcccgccggt agactctgcc
     1501 gatgcagaac gccttcaccg ggggtttggg gtgctccgcc aggtgctgga tggagaggct
     1561 cgtcgtatgg gtccggagca cgcactgctc ggcctttgca gcgctccagg tgcctcccca
     1621 cccggtggat gaggtctcgc cgccatgctc gtgcatatcg cggacatgct cataccctgc
     1681 cgggagcggc cagcgctcgc cgaggaagaa ggtgtcctgc atctcccggg ccgggtggtc
     1741 ctgcggctgg aagagggcgt cgaagttcca gaacgaactc tgcacgatcc cgcctgccat
     1801 ctctgtaaat cccatatcga ggaggacgcg gcgcatctcg tcgatgaggc gctggtaggg
     1861 gtggaccttc cccgggtaag cgcgtttcgg gagttttcca acatcgtagc gccggagagg
     1921 gaggtccttc catgagccgg agaggatctg ctcgcgggtg aacgtgcccg cctcttcctg
     1981 caggtcgagc cctccggcga ggagctggcg gccctgaggc gtgatcgaga ccgtgtaggc
     2041 gacggtctcc tcctcctcga cgagcccgcg ctttacgagg tcggcgaccc caacgccatc
     2101 ctcgatcgca ccggtctcac tgaggtgggc aaacgcggct tcgtcgggtc ccggagcggt
     2161 gttgccggtc ttctgcacga ccccgtcctt gatggcgatc cagcctttct tgcgcatcca
     2221 gccgatcgca atctttgcaa gcgggtggct ctggagatcc cgcatcggga ttgtctcccc
     2281 gaaactctcg atcacctgcc gctccgggag gcccttcccg atgtaagcct gtccttcttc
     2341 ggtcggggta taccgccggg agacacgctt ctccacgtcc acaagccccc gctcgccggc
     2401 gaggttcgca tattgcacca ccgcctcccg gcgggtttcc atcaggtccg cgagatcggc
     2461 cgcgtcggcc gatcccaccg gtcccagggc gaccaggagt ttcttctcat tcaacgtcag
     2521 ttccaccgta ccgcctcctc tgccaggtgt tcgactgcat ccatcgtatc ccgcaattct
     2581 ttcaggaaca tctgcacgcg ctccagcgtc tctttcttgc actgcccgca ggtgagttcg
     2641 ccgctctcgc accgccgccg caggtcggag agctccacgt cgtcctcgag catgtggaag
     2701 aggtttaaga ggtagaccga gcactggtcc ggctcgccgc caagacgcct ctgctcctca
     2761 agcgtcatgc gcccccccgt cagcgccgcc ataaccttct tcttgatcga cttatcggct
     2821 tcgtaaaacc cgaacgaact ctcaggcacg ctgctcgaca tcttcccgcc ctggagcccg
     2881 ggcatgaaga tgtggtaggt cgacgagggc aggtagaacc cgaacccccc atgggcgatc
     2941 tcgatctccc ggacggtgtc ctcgacccgg ttcatcggga ggccggtgac gtccacatgg
     3001 cccgcgtact tctttgatcc cacaaacgca cgggatactg cctcgatggc ttcctgcggg
     3061 gcgttcttcg accggacgct gacatggtcg ccgcggtcct cgaccgtgaa cattctgagt
     3121 ttataggcca cgtcccgggt gagccggata tgcgggtcct ggtcgaggcc gaccgggacg
     3181 atcgtaggag cgggcccggc gtcgagctgc gggaagagga tgtcgcccac ctgggtgatg
     3241 acgctcatgg cgtgggagag cgaggtctcg ggcccgaacc cgtatatcgc cgacatctcc
     3301 gagaagttca ccttcgtgga cgcctcgaac gcgaggtcct ttaagcgctc gttcctgctc
     3361 tggtagtagg ttgtgccctg aaagccgaga gcatagagag ccttgaggta ctccttcccg
     3421 aactcgcggc acttctccca cgagatgccg cggaccgcgt gcgcctcccg gtcggcgatg
     3481 gccacgtagc cgtttccgcc ctgacggacg tgccagacca cctccttcat gaccatcagg
     3541 tgcccgaggt gcgggaagcc tgagggcatg aacccggtca gtacgtgaaa cggggttttg
     3601 ttccggatgg cgtcgacgac caggttgtaa tcccggtgtc cgacgacaat tcccctgcgc
     3661 ataaatggcg gtacctcggg aagccttcgt gcgacctcac cgatcggctc gatgccgaac
     3721 tcggcaaaga gtcgatccac atcgacggtc tggttattcg accacgggtt catttcggaa
     3781 tgcatgtact gacactcaca gtttcattcg ggcgaattcg acgtatctga tatcggtatt
     3841 gtatatgcag gcaaacaaca tcttcttttt gacgctgtga gcaagcctta ccgagcggga
     3901 gacgccgctc atcggcgtgg aggtcccgga cggaacggcg tggacgagca tctccgagtg
     3961 ggtcttcttc ccggagtaga cgcggaagtg gtggccgaac ttgtacccgg ttctcgggat
     4021 gtagcccttc tcccggaggt cggtaaagac ccgttctttc cccaggatct cgacgtcctt
     4081 ctccgccgct gcatcgagga actgctctgc ggtcatcggt tcgccgtccc gcgtgatcga
     4141 gaggcattcc cggcgcatga gaaagatcga ctcgaccggg cggagcagca gccgttgcgg
     4201 gtcgagacgt ttgccgtacc agtcctcctc gagtggagtt cccggcggca ggtgggcgag
     4261 cgcataggtc ccgaagagag aggcctctac cggcggcatg ttgcacccgg ccggctcccc
     4321 gaccacggga aggtcctgca cccgcacctc gtagtaggtc agttcgtcct catcgtccac
     4381 gaccgcgagg aggtactgtt tgcgcatgtt gaccgcagca agcacgtcct ggctcagccg
     4441 atcgaaatcg acgaggtccc tctcagagag gacccggatc aggtactggg actttccggc
     4501 cccgggcttg tggccgcggc ggaagacacg gaaatcctgg ggaccgggct gaacgacata
     4561 tccccgctcg cggatatcgc ggtagacgag atacctccgg acaaagttcg gctggccggc
     4621 gaagagaccg agcagggtgt caaagccgaa gtctctcacg tcgatcttgc tgcgttccat
     4681 gaggtagagc gcctcttcag gagagagccg gaggccggtt ccctccagcc tcccgtagcc
     4741 gccctgctcg tagagcgtgc gtccctcgcc gctgaggcgc acccaggttc cgtcgaatat
     4801 cgccaacacg tgctcaaact atatggaagt tcacgttcat taatggattg ccggggggag
     4861 gaaccactac actttttgtg tgccgcaccg caacagtgat cgattccatg acgaaaagtt
     4921 accgaaccat ctggcagcca gggatgatcc tggcggcact gctcatcgcg gtggtgctgc
     4981 ccgcgggagt tgtctctgca gcagaggccg ctccgccggt cgcctgggac acgacgttct
     5041 caccggagaa taacagcaag ttcgatgccg ttgcaccgac cgcggacggg ggatacatcg
     5101 ctctcggctc taccctcacg caggtcttcg ggggcaggga agacctgctg ctcgtgaaga
     5161 ccgacggcca ggggaacggg atatggatgg agaggttccc cggcgtggcg gcagcctccg
     5221 ttgccgagac cgctgacggg gggtacgtca tcggcgccta caacgtctcc acgaccggct
     5281 cggagcagaa ctccacctac cagggaagct cgttcctgat caggaccgac gctgacgggg
     5341 cggaggagtg gcggcaggtc ctgccgggga tgaaagtctc gtccgtcgca gagacggctg
     5401 acggggacta cgtcgccatc gggtggctct ggaacccggc cggatcggag aacgacacga
     5461 cgacggtcat catgaagacc gacggcaacg gcacgccagt ctggaacagg acgttccccg
     5521 gtaccgcggc aaacgcaggg ctcgtgaccg ccgatggagg atacatcgtc gggggcacga
     5581 agtcgccctt cgagaacgac atcggggacg ccttcctcat ccgcctcgat gccgacggga
     5641 acacactctg gcataggaat tatggcgccc cggtcatctt cgacatcgag gagaccgatg
     5701 acgggctgtt cgtctactcg ggcaactact ggtacggcct tgtcgatgcg aacggagagg
     5761 aggtctggct caagaatata gagggcctga ccggctacgc cgtcgccctg cgacctgccg
     5821 gggggtatat ggttgcaggc acggccctcg gaaccgggga aggttttgtc ctcgggaccg
     5881 acgcggacgg gacaatccag tggaacatga cattccccgc tgccggggtc tacgcagcca
     5941 ccagtgctcc cgacggaggc tacacccttg cgggcatcca gttcctctcg cccgatacca
     6001 gcgccgcctg gctggtcaac cttgaggaga cggcaaatcc cacacaggcg gcaccgggat
     6061 tttgtgcgat cggtaccggg gcggcgctcc tcctcctgct cgcgggaagg aagcggagag
     6121 ggtaaccccc ctccaccact tttttctgaa ccgtttcagg gctcatcctt ccggaagtag
     6181 aggagcaacc ccacccccac gacgatgagg gcaagcagga cggggatgag gtgggcgtac
     6241 ggggagcctt gttcggtctc cgattgcggt ggggcgcctt ccgcgaccac ctgcgccgcg
     6301 ccgcccccgg gnngacggtg gtggggacct cttcccaggc gtccgccgac cggttgtacc
     6361 actgcaccag aagcccattt tgcccgctgt cattgtaaac ggcatcccac tgctcttccg
     6421 tgaagttgaa taagagggtc accggcggcg aaaaggcggc cccctccggc ccggcgatgc
     6481 aggcataccc ggcaaacgca tacgcgccca cgccgggcac ccggggtacg tcggccgggt
     6541 cgaggggctc aagggtcacc gccgcgagcg ggttgccggc ggcatcgaca gcccggacac
     6601 cctccgcgat cgtgagggtc acgaccccgt cggacgaacg gatcctggcg acctggtcga
     6661 cgaggccggt ctccccgagg tggagccgac cgggctcagc ctcttcgggg atggcggtcg
     6721 gcgtggaggt cggggtgggg gccgataccg gcgtgttcca gaccgggtta atccaggcag
     6781 agccgccacc accgccaccg ccactggccg gcggctgggt ctgggcaggt gtcggcgttg
     6841 ccgcggcggg acggacggat atgtatccgg tcttcaccac agtgctcctc ccggcggcat
     6901 tctccgcggt caggttgacg gtgtagttcc cggcggtggt ataggtatgc gaggggtgac
     6961 ggtcggtcga ggtcccaccg tcaccgaaac tccagagcca ggcggaaggc tccccggcag
     7021 aagtgtcgtt gaacgtaacc ttgatgggag ccgtgccgct gaggggagcg gcaccgaacc
     7081 ctgcggtcgg cgggccgaag gcgcggattt gcagggattg tgtggaatga tcgctgcctt
     7141 cggagacttt caggctgacg gtgtaggtcc cgggcgtctc gaatacgtgg accgggtccg
     7201 gcacggcaga ggtcttgccg tccccgaagt cccaggacca cgatccggga ctccccaccg
     7261 acgcatcctc aaaccggacc gcgagcgggg cggcgccgag ggtcacgttg gcggtgagtc
     7321 ccgcggtcag cgattcgcga accgtgatgt agccggtctc gaccgtggtg ttctctccca
     7381 catcgtttgc tacggtcagg gagacggtat aggttccggg cacctggtac acatgaacgg
     7441 ggctctctgc cgtcgacgat ttcccgtcac cgaaggtcca cgaccacgat aacggccccc
     7501 cctcagatgt atcctcaaac aggaccgcaa gcggggccct gcctccggtc acgtttgcgg
     7561 agaatccggc caccggcggt gcggtgaccg taatataccc ctccatcttc cgggtgtcgg
     7621 taccgagttc gttctcgacg gtgagcgtga gggtgtaggt tcctgcactc tgataggtgt
     7681 gggttgggtt ctgagcgttc gacaggttgc cgtcgccgaa ctcccaccgc cactcctccg
     7741 ggctgccggt ggattggtcg gtgaaccgga cggagagcgg caccccgccc accgttgtgt
     7801 tcgcctcaaa cccggcggtc agaggctcaa agactgctat atgaccgatc tttgtagtcg
     7861 tattctctcc ggcaccgttt gcgacggtca gggagacggt ataggttccg gggctcccgt
     7921 aggtatgctc cgggctccgg tcggcggagg ttcctccatc cccaaaactc caggaccagg
     7981 agaccggaac cccggtcgag gtatccgtga acctgacctt gagcggcgcg actcctttca
     8041 ccgtgttcac ggtgaatgcc gcgaccggcc gctcaatcac ggtgatgtag ccgctctttg
     8101 tctcagtatc gctcccggcg gagttcttga cctcgagcga gacggtgtag gtgccggcgg
     8161 cgagataggg atgctccgga ttctgctcgg aggagatccc gccgtcgccg aaacgccatg
     8221 accaggctgt gacatcgccg gtcgaggtgt ccgtgaaccg gacggtgagc ggggcggttc
     8281 cgttccgggt atcagccatg aatgaggctg ccggtggttc gatgacggtg atgtagtcgg
     8341 gtttcgtgat gtccgtactg cctgccgcgt tgctcgcggt caggctgaca ctgtaggtgc
     8401 cgactgccgc ataggtgtgc accgggttct gaacgtcaga cgctgcgccg tccccgaagt
     8461 tccaggacca ggtgtcgggg ctgtttgtcg actcatccgt gaactgcacg gcaagaggcg
     8521 cgccaccgga tgccggtgta gcggagaaac cggcggacgg aggctccggc agctctgcac
     8581 gaataaggtc ggtctctgtg gcggtgtcgg agccgaaccg gctgctcacc tcaagcgaga
     8641 cggtatactc gccggggctc tcgtaggtat gcaccgggtt ctgttctgtc gaccgatcgc
     8701 cgtcaccgaa ctcccattcc cactcctccg ggctgccggt ggattggtcg gtgaaccgga
     8761 cggtgagtgg agccgtgccg gtggccgggt ccaccgagaa cttcgcctcc gggggtgctc
     8821 cgccggcgta ggtgaaggcg tagaccttcc actctccgct ccgcttgtcc tgccagacga
     8881 tcctgtcccc ggagaccgag ggaaagagtt gctcgttctc cgcggtgcag accggcatct
     8941 cggctccgag atcgaagtca tacaggtaaa tatcccagag cccaccccgt ttatcctccc
     9001 aggcgatgat ctccccatcg atcgcagggg agacctgctc ggcaggatcg tcggtgatcc
     9061 tgtattcctg gttgctgcca agatcgtaca tgtagatgtc aggctgtccg ttccggtaat
     9121 cctcccagac gatccggttc cccgatatcg ccgggtatgc ctgctctgca ctgttccagg
     9181 tgatctgccg tttttgtccg gtctgaatag cataggtcca gatatctcca cccccggtgc
     9241 tctcctccca gacgacgtac tcgtccgaga gcgcaggctt ccactcggtc accggcgagc
     9301 aggagaggag ggtttcactg ccggtcgcga tgttgtagag gcagatatcc gcactccctt
     9361 cccgggtatc gtaccagacg acatgacccc cgtggacgac gggcatccac tggtctgccg
     9421 ggtcgtcggt gagcggcacc gtcgtgcccg ttgagtatct gtagagatag atatcccggc
     9481 tcatgttccg gttgtcctcc cagacgatgt aatcacccga gacggacggt ctcatctgtg
     9541 aagcaggatc gtctgtcctc tgttcaccgg ggccgggact gcttgctgag tagatattcc
     9601 tgtccccatc ccggccgtct tcccagacaa ccatatcgcc gtcgatatca gggtgctcct
     9661 gcaatccacc ggcagtacaa aagagcacca tatcgcccgg agccgtctcc accgccagag
     9721 caggcgggac cagcagtcca atgacgatga cggtgcagac taatatcagc gttttacctt
     9781 cagccatcct ccgatcacca cgatgagaac gccccctttc gtctcacacg atggtgcttg
     9841 caggcacgac ggagaagccc ttccccgccc ggcgtgcaaa cagagatccg gcggagtgcc
     9901 gggaatttcg gtattccgcc ggataaaggg ttcgtgaacg gcatgagggt ggtataaaca
     9961 tatatccctt gcggcgggcg gcttcctgca aacgccgggg agtattatac aaccatattg
    10021 aataaaaata ggagcaggct ccactccatc gtcatctgtg tttcagaccg attggacgcg
    10081 gtattcccgg ggcggacaac gccagggtgc tcactctccc gcaagttcac ggaccttcgc
    10141 gatgttcccg gcatcgcccc gccgctcgtc gaccggggtc tcgcagatga gagggaggcc
    10201 ccggacggcg gggtggcgga ggatctgccg gaacccctcc tccccgatgg acccgagccc
    10261 gacatgctcg tgccggtcaa gcccgctccc gagagcgccc ttgcagtcgt tgaggtgaac
    10321 gacccggagg cgggcaagac cgatcaggtc gtcgatctct gaggcgaccg cgtcgacccc
    10381 ctccgcggtc cggaggtcgt agccggcggc gaacgcatgg caggtatcga aacagatccc
    10441 gacccttcct tccgcgtcaa ccccatcgag aatgcggcgg aggtctgcaa cggtcgtccc
    10501 cacgctgttc ttctccccgg cagtattttc gaggagcagc atcacatcgc tctcgccggc
    10561 atcggcgagc gcctggttga cggcggcgat aacccgctcc tgccctgctt caacgccggc
    10621 gccgcggtgg tgcccgaggt gggtcacaag gtagggtatc ccgagaaggc cgcatcgccg
    10681 gagttccccg gtgagtgccg cgaccgattt ttcagcgatc tcggcatccg gcgaggccgg
    10741 gttcggcagg taaggcatgt ggtcgacgac cgggccgagc cccgatgcat tcaacgccat
    10801 cctgaaggca tcggctgagt ccgggtcgag gtcctttgcc ttccagccgc gggggttcct
    10861 agagaagatc tgaaacgtgt cgcaccccgc atcaagggcc ctgccgaccg caaggtcgat
    10921 cgaaccggcg atggagacgt ggcatcccac cagcaccata gcgtaactct caactctgaa
    10981 gcgtttaggt cttcctccgc ccgacctcgc ggagcaccgc cgccccgaac tcccgggtgc
    11041 cggcgacgcc gccgaggtcg cgggtccgga cgcccatccc gatcacccgg cagaccgcct
    11101 cctccacggc cgccgcggat gcagggtcgc cgaggtgcga gagcagcatc gcggcgctcc
    11161 tgatggctgc gacggggttt gcaatattct ggcccgcaat atcgggggcg ctcccatgca
    11221 cgggctcaaa gagggcggca cgctttccga tgttggcact cgggagcatc ccgagccccc
    11281 ccaccaggta ggcggcggcg tcggagagga tatctccaaa catattggtg gtcacgacaa
    11341 cgtcgtagag gtccggatgc atcaggaggt cgaggcagag cgcgtcgatg tagcgagccg
    11401 tgcagggaac tcccatccgg gcagcctccg ccgtgcagac ctcgacgaag aggcagtcgg
    11461 actttaacac attcgccttg ttgccgatgg tgaggtgacg gcgggatccg gcgagggtgc
    11521 aggcataccg ggcgatccgc tcgctccccc gccgggagac gacccggacg gtgcaggccc
    11581 ggtccggctc cgtccactcg atgccggagt agagcccctc ggtgttctcc cgcacgatga
    11641 cgatatcgac accttcgccc tgtatcgggc ggacgttcgc gtagaggtcg agagcatggc
    11701 ggatctggag gaggacgctc cggtagccgg ggtcgggcgg cgtcgtgacg gccccaaaga
    11761 ggatggcatc tgc
//
LOCUS       DAJB01000056            4114 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_73623, whole genome shotgun sequence.
ACCESSION   DAJB01000056 DAJB01000000
VERSION     DAJB01000056.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_73623"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          861 a         1192 c         1260 g          801 t
ORIGIN      
        1 ggagggatga tggggttggg gatggtgtat ccgcacggtt ctccggggtt attctcatca
       61 gcaacgggga agcggatgca gggtctagag cggctgctgt cgggcttgca accgctccct
      121 gactgctgga tcgtaatcgc ctgccacagt gactcccgga attctgggag tgcatgtgaa
      181 tgctcaggcg tccgtaactg cataatgcct ctgctgttgg gtgcccgaac tgccgggaga
      241 aaaaatttca gccaacggtc tgggattcgc cactcctgat tcccctttat tcaatatcta
      301 gataaattgg ggattaatta cattttcagc acaattctgc tataattccg aatcattgcc
      361 ttaaaataca aaatccatat atgaacggcg tgcagatcat ctggtaacta ttcagatgga
      421 aaggtgagca cttatctcga aaaaagcttt gataaccggc atcacagggc aggatggatc
      481 ataccttgct gaattccttc tccataaggg ctatgaggtg cacgggatca tccggcgggc
      541 atcgaccttc aataccgccc ggctcgacca tatctatacc gacccccacg atgcgggcgc
      601 acgactcttt cttcactacg gcgacctctc cgacaacgag cagatctcct atatcatcta
      661 caacatcaag cccgatgagg tctatcatct cggggcccag agccacgtgc gggtcagctt
      721 cgatacaccc gagtataccg ggaacgtcac tggcctcggg gcgacccgcc tcatcgagac
      781 gatccggaag agcgggaaca ggatgaagtt ctaccaggcc tcaagcagcg agatgttcgg
      841 cgcaacgccg ccgccgcaga gcgagacctc cgccttccaa ccgcggagcc cctatgcctg
      901 cgcgaaggcc tatgcctact ttatgacgaa gaattaccgc gacggctatg ggatgtttgc
      961 ggccaacggg atcctcttca accatgagtc gccccgtcgg ggcgagacgt ttgtgacgag
     1021 gaagatcacc cggggcatca gccggatcct cgcgggaaag gagaagtgcc tctacctcgg
     1081 gaaccttgag gcacggaggg actgggggtt tgccccggaa tacgtcgagt gcatgtggaa
     1141 gatcctccag cagggagcac ctgatgacta tgtcatcggg accgggggga gccactcggt
     1201 gaaggagttc cttgaagcgg cctgcgccta tgccggactt gactgcgagg agcacgtgcg
     1261 gatcgacccc aaatacttca ggccgaccga ggtggagagc ctgatcgccg acccggaaaa
     1321 ggcacgcacg cactttaact ggagaccccg ggtgacgttc cccgatctgg tccggatcat
     1381 ggtcgatgcc gacatgcggg cggcagggct tcccccaatc ggggaggggg acgaggtgct
     1441 ccggaggaag ttccccaacc ggtggtggga gggagactga ggtctttgtg ggtctgcatt
     1501 catccggaaa gccgggcgtg gcttagggag agtttcgcaa agatcgatgg catcttcaag
     1561 aagtacggcg caaacgaatg tagtatcata gccggagaga aaccatgaga tttggggacg
     1621 ggacgacggt tcttgtcacg ggcggggccg ggttcctcgg ctcatccctc gtgagggcgc
     1681 ttaaggaaca tgggcttgca gaagagaata tccgggcgcc gcggagccgc gaccttgacc
     1741 tgcggcggtg ggagaactgc gttactgcgg tcgagaacgt cgacctcgtc atccacctcg
     1801 cggcgaaggt cggggggatc ggctacaaca tggcaaaccc gggctctctc ttctacgaca
     1861 acgccattat ggggatccag ctgatggaag ccgcccggca ggccgatatc gagaaattcg
     1921 tcgccgtcgg gacgatctgc gcctacccga agttcacgcc ggtcccgttc cgggaagagg
     1981 aactctggaa cgggtatccc gaagagacca acgcgcctta cggccttgca aagaagatgc
     2041 tcctcgttca ggcccaggct taccggcagc agtacggatt caacgcaatc taccttcttc
     2101 cggtgaacct ctacggtccg ggggacaact tcgacccggt gagttcgcat gtcataccgg
     2161 ccctgatcaa gaagttcgtc gaggcggtgg agagcggtgc ggagagcgtc gaggtatggg
     2221 ggactggggc ggcgtcgcgg gagttcctct acgtcgacga cgccgcagaa gggatcgcgc
     2281 ttgccgccga acgctacgac aagcccgacc cggtgaacct cggagcgggc tttgagatca
     2341 ccattcgcga cctcgcgacc ctcatcgccg acctcaccgg gttcacggga gagatcgtct
     2401 gggacaccac caggcccgac ggccagcccc ggcggtgcct ggccgtctcc cgcgcggagc
     2461 aggagttcgg gttccgggcg aaggtcaact tccgcgaagg gctggagcgg acgatagcgt
     2521 ggtacaggga acgccgcgga tagcatgcgt atacaacgag atagcatccc acagaagaga
     2581 gagcagacct gaacaatagt gaatctcacc atgactgaac agacatcaga cttcgaactg
     2641 gtcatccgcc cgaagtatgg cctctttgac ataaactgga gggagctgaa ggagtaccac
     2701 gagctcctct acttcctcgc gctccgcgag atcaagatcc gatacaagca gaccgtcatg
     2761 ggagcgtcct gggcagtgct ccagccgctc ttcacgatgg tcgtcttcac cctgatcttc
     2821 ggcggcctcg cgcagatgcc ctctgagggg atcccgtacg ccctcttctc ctactcgggc
     2881 ctgctcctct ggacctactt ccagaacgcg ctgtctggtg cggggaacag cctggttggg
     2941 aacgcccagc ttctctcgaa ggtctacatg ccgaggatat tcatcccgac ggcgccatgc
     3001 cttgcgggga tcgtggacta cgggattgcc atgatcattc ttgccgccat gatggtctac
     3061 taccagttcg tcccgacggc agcgatcctt ctcctgccag tgatcgtctt cctgaccttc
     3121 ctcctcgcgg ctgggatggg ctactggctc tcatcgatct gcgtgaagta ccgggacgtc
     3181 aagttcgctc tcccgttctt catccagatg ctgatgttcg tctcgccggt gatctacccg
     3241 gcgagcatcc tcggtgagaa cctgcagtgg ctccttgccc tcaaccccct gaccggcctc
     3301 ctcaacgctc accgggcggt catcctcggc cacgtcccgg tggactatgt tggccttgcc
     3361 atatcggcgg caatcacgct tgcgatcttc ttcagcggcg tgctgtacct ccgccggaca
     3421 gagaaatact tcgcggacct ggtgtgacat gacatcccga aaagagaaac cgatcatcga
     3481 ggtgaagcac ctctccaagg aataccagat agggatggac cggacctaca agcggttcac
     3541 ggagagtttc acgaacgctg tgcggcaccc gatcaggacc ctgcgtggcc tcactggacc
     3601 gaaggagacc ttctgggccc tcaaggacat caacttcgag gtcgagcgcg gcgaggttct
     3661 cggcatcatc ggcaggaacg gcgccggcaa gagcaccctc ctcaagatcc tctcccggat
     3721 cacctatccg accgagggcg aggtcgtcat gcggggccgg gtcggctccc tccttgaggt
     3781 cgggaccggt tttcaccccg agctcaccgg ccgggagaac atctacttca acggcgccat
     3841 cctcggcatg aagaagcgcg agatcgacga gaagttcgac gagatcgtga agttctcggg
     3901 cgtcgagaag ttcctcgaca ccccggtgaa gaggtactca agcgggatgc aggtgcggct
     3961 cgcgttctcg gtggcggcgc acctggaccc ggagattttg ctggtggacg aggtgctggc
     4021 ggtgggggat gcggagttcc agaagaagtg tttagggaag atgggggagg tggcgagcga
     4081 gggaaggacg gtgctgtttg tgagtcataa tatg
//
LOCUS       DAJB01000057            4038 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_44995, whole genome shotgun sequence.
ACCESSION   DAJB01000057 DAJB01000000
VERSION     DAJB01000057.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_44995"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          825 a         1190 c         1273 g          750 t
ORIGIN      
        1 tccttcaggg cggggtagtt cactgggtga aaggtcatat caatgtagat tactccagtc
       61 tgacagacag tctggaaccg gaacgcggag atcagtatca tggaaaaggg aaaagtcgtc
      121 ctagcatttt cgggaggtct cgacacctcc atctgtatcc ccctcctgcg cgagcactac
      181 gggtttgacg agatcgtcac cgttgccgtc gacgtgggcc agcccgagga ggagataacc
      241 cgggcaacag agaagggccg gatgcttgca gacaaccact acaccatcga cgtcaaggag
      301 aagtttgtag cagactgcat cttcccgtcg atcaaggcga acgggtcata cgagggttac
      361 ccgatgggca cggcgcttgc ccgcccgctg atcgccgaag agatcgtgaa ggtcgcaaag
      421 caggaaggcg cgtcaaagat cgcacacggg tgcaccggga agggaaacga ccagctccgg
      481 ttcgacttca tcttcaggtc cgccgggtac gatatcattg caccgatgcg ggagatgaac
      541 ctgacccgcg agtgggagat ctgctacgcg caggaacaca acgtccctgt gaccgtggca
      601 aaggacaagc cctacagcat cgacgagaac tgctggagcc ggagcatcga gggcggcagg
      661 ctcgaagacc cggccttcca cccgcctgag gagatctacg cctggacggt atcgccactc
      721 gcggcccccg acgccgtgga ggaggtccgg atcgagttcg agcagggggt tccggtcgct
      781 ctcaacggaa agagactccc cggcctcgac ctgatccggg agttgaaccg gatcgccgga
      841 cggaacggcg tgggccgcaa cgacatggtc gaggaccgga tcctcggcct gaaggcccgc
      901 gagatctacg agcacccggc cgcgaccgtc ctccttgccg cgcaccgcga cctcgagcac
      961 ctcgtcctga cgcgctcgga actcgcgttc aagcatatcg tcgatgataa atggtcggag
     1021 ctcgcctata tggggctcgt gcatgaaccg ctcttctacg cgctcgacgc gttcgtcaac
     1081 aagacgcagg agcgggcgag cggcaccgtg gacgtcggcc tgtacaaggg cagcgtgaag
     1141 gtgctcggcc ggagctcgga tctcgggctg tactcggacg acctcgtctc cttcgactcg
     1201 acgacgatcg accagaacca cgctgtcggg ttctcgaatt atttcggact gcaggcacgc
     1261 ctcttccaga acctcaagaa gcgttgaagg ggtgcctaca gcaccttctt cccggcttct
     1321 tttcccggcg tgcactcgaa gacgtcggtg atcttgatga cgatgagcga ttttgccggg
     1381 tatttgtggc ccttcgcttt cacctggtca cggaacttct cgtaatcggc cccggacgtc
     1441 cggatctccg tcgagccttt tacctggaag cagcgcttgg tctccgcgtc ataaaagtag
     1501 agggccacaa cagggttctc tttgaggttt tcaagcgtct ttaccatgta gttgtccatg
     1561 atccagacgg tatcgtcggc gacgagctgt atcgatgcca tcggcgccac gttcgggacg
     1621 ccgcttttcg acgcagtagc catcggcatg actttggttt tgttgaatac ttccttaatc
     1681 tcacctgaaa gcgcaaccat acggttcacc tctccctgga agcggaacgg gcgcggcgag
     1741 gcgccaaaac cgtttccggt aatgtggtta tccgcgctat cgatatataa gatctctgcc
     1801 ggggtcgccg ttaccagccg ttttgccgtg aaccggtaac gacggagtac cttcccgccc
     1861 gcactggcag tccggaaatg cggggcacaa gcgggatggg gtctccctct tcccgaatgt
     1921 taccccttcg ggtctctcaa actcctgccg atccgctaac gctaaagtcc cccccgaatc
     1981 ggaaaagact tcctggaggg caggtattcg accatccggc cgggaggagg aagccagccg
     2041 gagcgttcct gctctccgtc ttttagatgc cttatggggg ttatggaaaa atataagcgg
     2101 gcggacaggg aaaagactac tatgtgtgcc cgctttctgg agcgcttttt caaacctacc
     2161 ccgcacatcg tcgaaagccc gcccccgccg tcgctctccc acggaccggg cacaggtgtt
     2221 ccggagtacc gggtgaagcc ctacttcatc gtggcgtctg tcgagatggg caatactacg
     2281 acaaagtgca ttctgaccgg cacgaacctg gaaacgggca ggtcctacgt catcaacaag
     2341 accgtgacca tgagccgtga tgtcagacca ccaaaaccgg gcgagacgat cttcggggcg
     2401 accctggacg gcacggaact gacgcgggag tcggtcacgg aactggtgcg cgacaccctg
     2461 ctccagtgcc acaaagacgc tcacctgagt atcaaggacg acctggactt cgtcgtcagg
     2521 agcaccggcg tcgtggcggc gatggattcc cccgaccagg tcggggactt cgtaatcgct
     2581 ctcgcgaacg gctgcctcga agccggggtt cccccgcgga agatgacgcc gccgatgtcg
     2641 atcgacaacc tcccccccaa actccggcag ttctcgtttg ccgacaaggt ggtcttcatc
     2701 ggcgcggtgg ccggtgtcgt gccgccgatc gggtgtaccg gcgtcgagat ggtggcaaac
     2761 gagatggaag gggagctcgc gatggcgggc atcaaggagg gcgccaagtg gacgccggtc
     2821 gatttccgga acccctgcat atccatcgac ttcgggacca cgctcgatgg gcggatcacg
     2881 agcgacgtcg ctccggatga gcccaacccc ttcgcccaga cgaccgggaa cttctgcggg
     2941 cttgcaggtg ccataccgga tgcgatcgtc cggggcaccg ggcttgtaaa gaaccggacg
     3001 gggacggccc tcgacctctt cggcgaccac agcattcagg gcgcgttcgg gggcagaaag
     3061 cggtccgtcg tcgacggcta cgtcgaccgg tgccacgagc atatcgatat ccggatcgtc
     3121 ccccccgacc ggacgaggtt cgggcgggtc ccggtctgcg ccgaggtggc cgacaaatcg
     3181 ggcgttgccc ttatcgggtg cgactcgggc gtcaacggca gcgagatgcc ggctctcgag
     3241 gagatcggac acgagatcca cgagaagcac ggcatggcca tgctgaccga ggtcgtcgac
     3301 cgggtctgcg cgaggatggc gctccggctc atcgatgtcg ctatcgagag ggggatggcc
     3361 ccgccgaact cgtcgatcgg gttcaccggg cgggcggcga tctcggggag gaagcccgag
     3421 tacatcctcg ccggcatcac cgaacggaac ctctacgaga acccgaacga ccacctggtc
     3481 tttgtggacg acggccttgc ccggggagcg gcactgatgg ggcggtgcat gaactcgctc
     3541 gggaagccga agaccccgct cggcggcgtg cggggagggc catgtattat gtcccgccgg
     3601 atcaaaatag gaaagtagga tatggtaatg atggctgaag aagaactcta cgatatgctc
     3661 gtccccccgg gtgtgccccg caagatgatc tacgacgtcg tcgataagtt cgacgtagaa
     3721 gtggttcggc ggcagcagaa gctgtcgttt gcgaacatgg acggggacgc gagagaactg
     3781 attgctttcc gcggcaaaag agaggtcgtc gaagaggtgc agaagtacct tttcgagcag
     3841 ctcaaagagt tcatcgggga ataactacac ctttttttgt ttgtgcaccg ggaattgccg
     3901 gtgtggggca tagccccggg gtgaccggtg gaaaccgggg taatgtgtag aggaatgaac
     3961 tgaacccggt tttctccgac tcacccgtac actggaccgt gggggtatcg cccttggggg
     4021 aggggctgac ggggaggg
//
LOCUS       DAJB01000058           13587 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_49445, whole genome shotgun sequence.
ACCESSION   DAJB01000058 DAJB01000000
VERSION     DAJB01000058.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13587)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_49445"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2983 a         4069 c         4049 g         2486 t
ORIGIN      
        1 tagcgcccgc ccccacacgc caaaattcct aaaataaact gacaagatgc actagtacag
       61 ggcggcagcc ggccgggggt aaccgagatc cgggatgccc gcgaatcagt tcgacagaga
      121 tctttcccca atctatcccc aattaagatt ttaaatacca attcattgag agttattatg
      181 cgatccaggg taaattattt gagataattt ccccaatctt ctccaatcga tccccaatct
      241 tctccaattc atgtttttgt aacatctaag tattgacgca cattttgcaa ttcaatgcag
      301 gatatttgag ataatttccc caatcttctc caattggatc cctaattata ttccctccag
      361 aggggagggg ttgagcaccc atttcgcact cctcgtcttc ccgagggtca tgatcgtccc
      421 ttcctttcgc atctccttca agagagactt gatgaactgg cgcttctgtt cctcgttcaa
      481 agcatcggaa accttgtcca gcaacagtaa ttcaatctcc gccctttttg cctcgtgata
      541 ggttcgaaga taagccacaa tcaggtcctt gaaatatgcc ttgtcaaacg atcgcttccg
      601 gatatagtca gcccgggtat cggtctcggc cgcgacactc tccgagacgt acaggttcag
      661 atgccgcccc tctatgagtt tcttctttct cagagcgttc cgttcctccg aggtaacggg
      721 atagcccttc tgcaccttat cgagagcgat cacatccatc agcgtcagat ccttccgctt
      781 gatgagcatc cgggtgtacc gttcatcgat cacccgacca gggatcgtga cccgcaccaa
      841 acctatctca ccaagatcat aatcaggcat cgggaaatta cggtcccgct gtttccggaa
      901 catccgcttg atcccgctgc cgatggtgtc gatcatgttc aactccacca tcgcactggc
      961 aaggaaggga tttcgatacc ggtccggagg cgtatcgcgt tcgatcacac tctggacagt
     1021 tccgggaatg aaatcgccgc gattggtaaa catcagggag tccggcctct cggtcaccgt
     1081 aatgcgggca gactgcaagt agtcctggtg agcaatgcaa ttgtgcaacg cttcacggat
     1141 cacccacggg tcgtattgcg tcacctcaag agggaacagc gtctccccaa aaatatggcg
     1201 gatggtgagg ttccggatcc tggcaaatac tctgtcgaca gcaaggatca gcggcaaccc
     1261 gaaatgggca tagtcgatct ccaccttgtc cttatccctg agaacccagg tgacgtgtgc
     1321 aattgcaggt gagagaaggt gtgcggcctc actctttccc aggaggacaa ttgcggcatt
     1381 cgtaagccga ccatgactgc agacttttgc cctggcaagg aacgaaaggt catcccactc
     1441 gtcgacttcg cctgccaggt tctgatgctt ctccttgtat ttttcccgcg caaaggcgat
     1501 cgcctcagga tcgagatccg catacgtcgc cccctcacag accttcgccg accagtcgtc
     1561 cagaggggcc tgccggcgta tctcttcgat ctcgtggagg gcaagaggtc cgagggactc
     1621 atggatacga ccatacgcaa cacccttcca tgtagtcggg ataccgcgcg tcgccgcagg
     1681 aatctcaaac atcaccgctc gcattccatc gaccgtgagt tcatggacac cggcaaacgt
     1741 catctggtga ttcgtatgct gtgcgatctg ctctttgagg cggtcgaggc caggaggagt
     1801 gaggcgataa tttgagccaa caatctttcg gggcgacctg tcggtgatgc caaagatcag
     1861 ccagcctgcc gggtttccat acaggttggc ctcgttgctc agtgccgaga aatacctgcc
     1921 tacatcatcg aaatcaaagt tggtctttgc ctccttgaac tcgatgcatt cggtttcggc
     1981 cggaaaatcc atcatctccc gcagttgaac aaccagatcc tcagacatcg ctctctcctc
     2041 ccggtcactc tgttcgcata attgattgag tcagttcctc cttaagcctc tcccttgcca
     2101 cctgcacggc cgccggcatt ttgcacagtt gctgggttct acaacctccc ggccaccagc
     2161 atcattggac atcgccggcc tcttcgacgc gacgacgaac aggccgccct cctcgccctc
     2221 gtccgggagg aaaaagactt cctcgatgac cggtcccacc ccgacggcta caacgtcggc
     2281 gaggccgctg taacgtccgg cagggagcgg gaattgcttc aacccggcgg aggggggccg
     2341 cttcgccgcc ttcgccacgc gttatacgtt cgccgcgagt tcgacccgct tcaggacctg
     2401 cgaccgatac catgcgagga tctttaaaac gatctcataa cccgtcccct tcaggcgata
     2461 cgataccgca gactgactgg tgccgacccg gtccccgatc tccttctgcg tgaacccttc
     2521 cagcgtcagc atcaccgctt cagcctgcct cttcgtatac ccgccgatgg tccgatccac
     2581 gacatcgcag aagatcgaga gcgtctcatt caggtcttcc cagggggtcg ttacgatgat
     2641 gccgcgcccg gctttcttca tggcgtccag ttcgaggccc gagagccgga atgcctcgcc
     2701 atccccctcc ccggccgaac catcgcccgg caggtagcgg atggtcccaa ccccgaccgc
     2761 aatccttgca tccagccgga gaacgctgtt gtcgcgatcg aacccggcga tgagcttgag
     2821 gcgcaggagg accgcatccc tcagtgcctc ttctggatcc gaagagacgc cctgaaaact
     2881 gtcgccgcgg aagattgaga acggcgctgt gtagtctccc ccgatctccg ccagtatctc
     2941 ctttaagcgg cgtatgcact cttcaccgca tgcatccttg atcactctgg agcggaccag
     3001 atcccccgtg aggactgcgt acactcttct tccggtcatg acgtcatcgc tgcctactct
     3061 tcaaaattcc ttgtccgccc tctgatatga atatatctac tcataaccag aaaatatcag
     3121 tatattaact cataaattat cagcatgagt ataattacga tttctcctat tctttccgga
     3181 gagattgggt atgtacgcag ttacggcatc tggagcagtt cagagagtcc atttctccag
     3241 gatgaccacc cgccatcggg actgcggtag ggaagcgcga tctctaaagg ggtccaccac
     3301 tcctcggcgg ggacacgccc gtcccgcacg atgaaaaagg atgggctatc ggtctgcaga
     3361 agaactgacg gacatccccg cttccccgtc cggggtcagg taccgcatct tcagcctgag
     3421 gatccgcccg caggactcgt cgatccgctc ctccgggatc tccccggctt cgacgaggtc
     3481 cttcacgatg ccgacggcct tctccgcgat tctctcatcg tagaccaggt tgttcgcaaa
     3541 gaggaagatg tcgcacccgg cgttgatcga gagtttcagc gactcgttca ggtcgtagtt
     3601 gtcgtggatg gcgcccatgt ccatcgcgtc cgtgacgacc acgcccgaat aaccgagccg
     3661 gtcccggagg atgccggtga ccgtcggctc cgagagcgtc gccgggtagt cggggtccag
     3721 gttggcgttg tagacgtggg cggtcatcac catgtcgggg aggccccggg cgatgaggtc
     3781 ccggtagggc aggagttcct cctcggtcca ggtctgtgtg acgtcggtga acccctcgtg
     3841 cgtatcggtc atggcgctcc cgtggccggg gaagtgcttg atcgctgtca tcacgccgag
     3901 gttgtggtgc tcctcgatga cccaccgtgc gttttcggcg acgacggtgg cgttctcaga
     3961 gaatctccgg ttgagtctcc cgatggcggg cgagtcgggg ttgacggcga ggtcgacgac
     4021 cggtccgaaa ttcagattga acccgttctc cccgaccatc gccgcaagcg cccgcccggc
     4081 ttcccgggtg gcggtctcgt tctcctgcgc cccgagcgat cgggccgaga ccgtcggggg
     4141 gaacccggag gactccttca gacggcagac cgccccgccc tcctggtcga ccgcgacaaa
     4201 gatcgggatc gctccggcgt agccctggag cgaggcggtc agggcccgga cctgggcggg
     4261 gtctctgatg ttcctctcac tggtgccgag cgccacgtcc cggtcgaaga ggatcacccc
     4321 gccgacacgc ccggccgcga tgtcgcgggc gatctgcgaa tccgggtcga ccgagtagcc
     4381 gcggaacccg acgaggagca tctgcccgat cttctcctcg agggagagcg agtcgagggt
     4441 ccggttgacg gcgtccgcag tccccggcgg ggcggacgtg cagcccgtga tacagacagc
     4501 gaggatgacg acgaggaggg cggcgaggga gcggttgagc atactattgg atgaaatctg
     4561 ccggaataaa tgcgtgcctt acgggggggc ttgtcgtcgt tacctctcca gagccggtcg
     4621 caatgctgcc ggatgccggt cacggacccc cggtccccga ccgggcgccc atgaggccgg
     4681 gagagaaccg ccggatcgtc gatccggagg acgcccggct cggacctgct gcccccggcc
     4741 gtgcagaacg caccggccga tcggccaccc atccccgcca ggttccacat gccgccggtc
     4801 aatccctctt ttcgccgggc tcccgtacga cagccgcccc tgcgcgtcga acgggttttc
     4861 ccctgtcgat cccggggtcc gggtaacgcc cgcgtactcg cgttccccgt tgccgttgcc
     4921 ccgaaaccgg aggctatgcc gggcacaata gatatattta tataaggtag aaggaagttt
     4981 ccttccgaaa caaataaata tctgcatgca gacatttcag gtggcaacgg aagaggaaaa
     5041 cagcatgaga caagttgcaa tctatggaaa aggcggtatc ggcaagtcga cgacaacgca
     5101 gaacacggtg gcggcgctcg cggaggccgg taagcatgtg atggtggtag ggtgcgaccc
     5161 gaaggccgac gctacccggc tcctgctcca cggattgtgc cagaagacgg ttctcgacac
     5221 cctcagggac gaaggcgacg acatcgatct cgatgagatc ctcaaaaccg gattcgggaa
     5281 catccgctgt gtcgaatcgg gcggtcccga acccggggtc ggctgcgccg gcagggggat
     5341 catcacggcc atcaacctcc tcgaatctct cggagcatac acccccgacc ttgactatgt
     5401 cttctacgac gtgctcggcg acgtcgtctg cggcggtttc gcgatgccga tccgggaagg
     5461 gaaggcacag gagatctaca tcgttgcatc gggcgaactg atggccctgt atgccgcgaa
     5521 caacatcacc aaaggcattt acaagtatgc cctgaacggc aaagtcaggc tcggcggcat
     5581 catcggcaac agccggaacg tggacaacga gctcgaactc ctgcaggcgt tcgccacaga
     5641 actcgggtcc cagttgatct acttcgtccc gcgcgacaac ctggtgcagc gggccgagat
     5701 ccacaagaag acggtcgttg acttcgatcc cgaggccaac caggcgaacg agtaccggaa
     5761 cctcgcagag gccatcgatg gcaacaagat gttcgttatc cccaaaccca tggctcagaa
     5821 tcgtctggaa gaactgatga tggagcacgg gttcctgggc ctgtagacag gacgtgaagc
     5881 agatgcagtt tgtacgagcg atagtcaggc ctgaaaagaa ggacgaagtc cttgaaaacc
     5941 tgtcggcagg gggattcaac gccgcgacgg ttgtcgacgt cgtgggtcgc ggcaaacaga
     6001 agggcatcag gatgggggac gtctactacg acgagatccc caaaaccctc atcctcgttg
     6061 cgatagagga cgaggacaaa gacaggatcg tggatatcat cctcgggacc gccaggaccg
     6121 gcccgagcgg caacttcggc gacgggaaga tcttcatcag tcccgtggaa gaggcgtaca
     6181 ccgtctcaaa gggtgcaaaa ggtctctgaa ccggcggtgg tgaaatgaag gagattatgg
     6241 ccatcgtgcg aatgaaaaag accggtgcga cgaaacgtgc cctgatcgag gcgggcgtcg
     6301 ccggttttac cgtcgtcaaa gcgatgggca gaggaaaact gctgccgcat gacgaaataa
     6361 acgtcccctg caaggaaaaa ctcatgggga tggtcgctac cgacgtcatc gacagggagg
     6421 aatccgaaga gcaggtcgtc gcgttcctcg acgacaaaac gatgtttccg cggcggttgt
     6481 tcaccatcct cgcctacgac gaggatgtgc cgcggatcgt ggaggcgatc gtcagggcca
     6541 accgcacgga gcgcggtgca ggcgacggaa agatcttcgt gacgcctgtg gccgatgcag
     6601 tccgtgtccg cacgggcgaa tccggtgacg ctgcaatctg gtaagaacta caggagacac
     6661 gacacatgac gatgacggat gtaaacgtcg acgagctgct ggcaccgatg ccggataaag
     6721 taaaaaagaa caggagaaag cacatcgtca aaaaggatgt atccgccggt tcctgcccgc
     6781 agatcgaggc aaacaccagg acggtccccg ggatcataac gcagcgggga tgctgctatg
     6841 ccgggtgcaa gggcgtggtc gtcggaccga tcaaggatat gctcacgatc acccacggcc
     6901 cgatcggatg cgcatactac agctggggaa cacggcggaa caaggcacgg gtcgacgaca
     6961 gaacccctcc ggagcagatc tactcggcga tgtgcttctc gaccgacatg caggagagcg
     7021 acatcgtctt tgggggcgag aagaaactcg cgaagatgat cgacgaggtc gtggcgatct
     7081 tccacccgag ggccatcaac ctctgtgcga catgcccgat cggacttatc ggcgacgatc
     7141 tttccgccgt tgcaaaagag gcggaggaga ggcacgggat tccggtgatc cattacaact
     7201 gcgagggcta taaaggagtc agccagtcgg ccggccacca tatcgccaac aaccagatca
     7261 tggaacgcat catcgggacc ggcaccgaga aggtggacgg gaaatacgtc ctcaacatcc
     7321 tcggcgagta caatatcggc ggcgacgcct gggagaacga gagactcctt gaagagatcg
     7381 ggtacacgat aggctcgacg cttaccggcg attctgccta cgtaaccatc agaaacatgc
     7441 ataaggctca cctgaacctc gtccagtgcc accgttcgat caactacatc gccgagatga
     7501 tggagacgaa gtacggcacc ccctggctga aggtgaactt catcggtatc gatgcgacga
     7561 tcgatacgct ccgcgagatt gcacggtgtt tcggcgacga agaactgata gcaaggaccg
     7621 aactcgtcat agagcgcgaa cttgccgcgg tcacgccgga gatcgagaga taccggaaga
     7681 tcctcaaggg caagaccgcc tttgtcttcg tcggcggatc gcgcagccac cactaccagt
     7741 tcctgctcag gaatctcggc atggaggtca tggttgcggg gtacgagttc gcccaccgtg
     7801 acgactacga ggggcgcgag gtcataccga ccatcaagag cgatgcggac tcaaagaaca
     7861 tccccgagct ccatctcgaa ccggaccccg ggatgtacag gccgccccac ctgcacctga
     7921 agatgtcgaa ggagaagtac gacgaactgg tcgcatccgg cgtcctcaac gactacaagg
     7981 ggatgtaccc ggacatggta aacggcggca tcatgatcga cgacgccaac cacttcgaga
     8041 ccgaagaact ggtcgagatc ttcaagcccg acctgatctt caccggcata aaggacaagt
     8101 acgtcacgca caagatgggc ctcccctcga aacagatgca ctcgtacgac tacagcggcc
     8161 cgtacacgtg ctttaggggt gcggtgacct ttgcaaagga tgtagcgcat tcgctctcca
     8221 cgcccgcatg gaagatgatc accccgccct gggagcagag caagaccgaa cagaggtgaa
     8281 ccaacgatgc tcgactgcac accaaaagag cctctccctc aagatgcacg tacaacagga
     8341 aagatcaacc cgataaagac atgccagccg gtcggtgcga tgtatgcagc cctcggcatt
     8401 cacggctgtc tcccgcacag ccacgggtcg caggggtgct gttcctacca ccggatggcg
     8461 ttgtccaggc acttccacga gcccgcgatg gcctcgacga gttccttcac ggaaggagca
     8521 tccgtattcg gcggcgctgc aaacttaaag accgcgatca agaacgtatt cgcgatctac
     8581 aacccggaga tcatcgcagt ccacaccacg tgcctcagcg agaccatcgg ggacgacatc
     8641 ccgaccatca tcaagcagtc ggagatcccc gaaggcaggt acgtcatcca cgccaacacg
     8701 ccgagttacc acggctcgca catcaccggg ttttcgaaca tgtgcaaggg gatcgtatcc
     8761 taccttgcag agtcgaacgg cagcccgaag aaggaacagg cgaacatact cccggggttc
     8821 gtaaaccccg gggatatgag ggagatcaaa cggatcgtaa ccgagatggg cgttccctcg
     8881 atcatgtatc ccgacaccac gggcgtgatg gactcgccgc tgcgggacac ctacgagatg
     8941 taccccctgg gcggtgcggc aatcgaggac ataaaagact cgggcaactc gaagatgacc
     9001 ctctcgctcg gcgcatggtc gtcggatgag gcggcggcga ctctccaggc gaagtgcgga
     9061 gtgcccggcg tcccgctcag gatcccgatc ggggtcaaag cgaccgacga cctgatcatg
     9121 gcagtgaagg aagggttcgg ggtagacgtg cccgaatccc tcgaagtcga gagagggcag
     9181 gtcatcgaca ccctgatcga cacccacttc cagtacgaag ggaagaaggt ggcggtattc
     9241 ggggacccgg acatcatggt cccgctcgcg gagttcctca tcaccatggg catgatcccg
     9301 aagtacgtcg ttaccggaac gccgggcaag aagttcgaga agaccgtcaa cgggatgttc
     9361 gaagacgcgg ggatagaagg aagcagggtc agggccgagg gagacctctt cgacctccac
     9421 cagtggatca aggaagaatc ggtcgacctc ctcatcggca acacgcacgg caagtacatc
     9481 gcacgggccg aggacatccc cctggtcagg gtcgggttcc cggtattcga ccgggccgta
     9541 cacccgctga tgccgatcgt cggctaccgg gggtgcctcc ggctgatcga gatgatcagc
     9601 aacgcactgc tcgagcggcg cgaccgcgac tctctggacg aagattacga actgatcctg
     9661 taagattggt gaaagataca tggagaacga atgtttggtg cccaccgagc ctacggcatg
     9721 catcgatgag aggcagttct ccatcatgac cacgggaaaa aacaaaagcc gcattcactg
     9781 cgctgatgac agtctggccg gggccgtgag ccagcgtgcc tgcgtgtatt ccggggcgag
     9841 ggtggtctta aatccggtga cggatgcggt ccacctcgtc cacggcccca tcgggtgtgc
     9901 ggcgtacaca tgggatatcc gcggcagtct gtccagcggc tcggagatgt ttcggcagag
     9961 tttttccacc gacttaaaag agagggacgt ggtctttggt ggagagaaga aactggtcgc
    10021 ctgtatcgac gaagtcgtcg agaagtatcg ccccccggtg atattcgtct acgcaacctg
    10081 cgtcgtcggg ttgatcggcg acgacgtcgt cgccgtctgc aaggaggcgt cagagagaca
    10141 cggcatcgac gtgatcccgg tggagtccag tggcttcctc tcgggaaaca agatcgtcgg
    10201 ctaccgggcg gcaggagaag ccctgctgaa gctgatccgc ccggagggcg gggagaccgt
    10261 cgaaaaaacg cggaaactca atttcctcgg cgaatacaac ctgggcggcg agaagtggct
    10321 cgtcgagcgc tacctccggg agatgggcgt cgagatcaac gtggccttca ccggggactc
    10381 gaccgtcgca gccctgaaga aagcacccgg agcatgcctg aacctggtgc agtgcagcgg
    10441 ttccatgcac tgggtggcga tgagcctgga gacggagtac ggcatcccgt acatcgacgt
    10501 aaacttcttc ggcgccgaga acaccgcgga aagcctgcgc aagatcgccc gtttctacgg
    10561 ggacgaggag atcgtgcgcc ggacggaggc gctcatcgaa cgcgagatgg agagagtcag
    10621 gccggtcatc gagatgtacc gggaaaaact ggcaggaaag agggcggcga tctacgtggg
    10681 cggcgccttc aaggcgctcg ccatcatccg ccagttgcag gagctcggca tggaggtcat
    10741 gttcaccggt acgcagaccg gcaagcctga agattacgag cagatcggcg ccatcgtcag
    10801 cgaggggacg gtcgtgatcg acgacgccaa ccccgccgag atcgagaggt tcctgctgga
    10861 aaaagaggtc gacatgatgg ccggcggcgt caaagagcgg ttccttgcgc acaaactggg
    10921 cgtcggcttc gtcgaccaca accacgaccg caaagacggt cttgccgggt tcgacggagc
    10981 gatccggttc gcccgcgagg tgtacgccac cacctgctcc ccggtctggg aacgggtgaa
    11041 gagaaacccc ttcgaggagg cagagggatg atcgaggtca cgtcacgggc aaagcaggtc
    11101 aacgagaacc agtgccacat gtgcatgcct ctcggcgggg tcatcgccct ccggggcgtc
    11161 gaagactcca tcgcactggt gcacggttca caggggtgca gcacctacat gcgtcttgcg
    11221 aacgtcgagc acttcaacga accgatcgac atcgcctcct cgtccttaaa cgagaagcag
    11281 gcgatcttcg gaggggaggc gagcctgaga aaggcgctgg acaacgtgat ccgggtctat
    11341 cggccgaaag tcatcggcat cctgacgagc tgcctcgccg agaccatggg cgacgatatc
    11401 gcgaggttcg tcgagaccta ccgaacggac agggagatca ggggggtcga gatcatcccc
    11461 gtctccacgc cgagttacag cggcacgcac accgaggggt tctgggcgac gacgcgggcg
    11521 gtcatcgccc actatgcccg gccgaccgaa cgccaccggg ggatcaacgt catcgtcccc
    11581 aatatcagtc cggccgacat cagggagata aaacgcctcc tcgacctcat ggggctggag
    11641 tacacgctgc tgcccgacta ctccctgacg ctggatcgcc ccttcggagg acaataccag
    11701 aagatcgcgc cgggcggcac gccgaccacc gcaatcgcac gcatgagcgg agcacgggcc
    11761 accatccagt tcggcctcac ctgcccggac agcctctccc ccggcctctt cctggaagag
    11821 cggttcggcg tcccgctgat caacctcccg ctccccatcg gcctcgacgg cacggacctc
    11881 ttcgtcggga ccttgaagga gatcggcggg cagcccgtgc ccgcgagcct catgctggaa
    11941 cggggatggc tctgcgacgc gatggccgac gcccataaga tcaacgcggc ggcgcggccg
    12001 gtcatctacg gcgaacccga actggtctat gccctggcaa aactctgtct ggagaacggt
    12061 tcggttcctg ccgtcatcgc cggcggaacg cgcaacagcg gccttaccgc gctgctgcag
    12121 cccctgctcg acgacgcgct ggaagagccg accgtcctcg aagaagcgga cttcgtgacg
    12181 atcgaggagg cagcggtgcg gacgtcggcg aacctcgccg tcggccactc gggcggcagg
    12241 tacctgaccg aacggcaggg gatccccgga ctcagggtgg gctaccccat ccacgaccgg
    12301 gtcggcggac agagaatcct gtcggtcggg tacacgggca ccctcgccct cctggaccgg
    12361 ttcaccaaca ccctgctgga agcgaagtac ggttcctacc gcagacgacg gaaagaagag
    12421 ttgctcaacg aaaaaggtgt atccgatgca aaagcctgaa cgtcacattt tcatctgcac
    12481 gagttcacgc gtaaacggtc agcagaaagg gttctgccac tccaaggacg gcgtggccgt
    12541 catgatgaag ttcatggaag agattaaaga tcgcgaactc ggcggcgagg tgttcatcaa
    12601 caacaccggc tgtttcggca tctgcgagaa ggggccgatc gttgtggtct acccggacaa
    12661 cgtctggtac gggtccgtta cgcccgaaga cgtcgaggag atccttaata agcatttcga
    12721 aggcggcaac gccgtggaac ggctggtgat ttaggacatg tgcaaaagat gtgaagatca
    12781 cggcagcgtc ggggaggttg cagtactcct cgatgcagac ggtttttccg gcaggttctc
    12841 cgaacccgga aagattgtcg tgtacagaaa gtgcggaccc tcctttacgg ccgagcgcga
    12901 gatggagttt gctctcgacc cggacgaggg gctcgcggaa ctgcgctcca gaatggacga
    12961 acttctacag ttcctcggtc catgcaggat cttcgtcgcg aagtcggcga gcggcgccgt
    13021 ctacttcgag ctcgagaagg cggggtgcag cgtgtgggag gtctccggca gaccggacga
    13081 gtttctcgac agcgtgctga agggcgagga ggaggcggag agcgcagcgg caccggccgt
    13141 cgagatcccc gtgcccgtcg aggtctcgcc cggaaactac ttcatctcga taaaggaggt
    13201 ccagggaaag gcgcccttcg tcagcagcaa gcaggtcctc caggagttca tatgccgggc
    13261 cggtttcgat gcgctggagg tggtctgcga tcacgtgccc ccctggatcg agatggaagc
    13321 ggaacggaga ggatatgcca tggagaccga gccgcacggg aagaacgagg ttcacgtgag
    13381 gcttgcgagg ggtacggccg ctcgatgaga ggccgtctcc tgcaatcgat caggacaact
    13441 ccggctacac ctacccacgg ctttgttgaa accacacctg tttgtcccag atggccgcaa
    13501 tcagtggtac tcaggggcac ggctttccac cgggtatcgc catgactgcc cccgctctgg
    13561 ggcgggggag gaggccggag gccgggc
//
LOCUS       DAJB01000059           11526 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_54019, whole genome shotgun sequence.
ACCESSION   DAJB01000059 DAJB01000000
VERSION     DAJB01000059.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_54019"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2155 a         3778 c         3398 g         2195 t
ORIGIN      
        1 acgaccagga catctccttc ccgtatccag accttcttct tgatcttgcc cttgatccgc
       61 ccggtgcgcg tcaccccgtc gaagcagcgg atcttgatat gatttgcgcc cagcatcagt
      121 tcagcgcttg cgaacatctc ccggttcttc ttgttcggca ggcgcacgcg gataatctcc
      181 tctcccgcct cccctccttt ggactcgggt ttgcgctttg caggatctgt cagttcatca
      241 accccatgct ccagagtatt tgctctcttg tgtgtccagt ttatacaact gctagcttat
      301 caaatattct cccggaggcg ggaaggtgac cgcaacggcc accggtcgcg ccctggttgg
      361 ccgtgagatc acccggacgg aacccccggt cgatcacccg gacccgggag accgccaccg
      421 ggacggttat gtatattgcc gggactagct acccagcata cccatggcat gcccggaccc
      481 gtcgcgcggg atctacgtcg cctacttcat ccaggggctc atcctcctga gcgcagcctc
      541 cagcatcacg gcgggcgagt acttcctcgg gctctcagcg ggagtcgcct tcctcctgac
      601 gatggcgccg tccctcatga cgaggaacct gcgcctctgc ctgccctggg aggtcaacct
      661 tctcgtcgcc gtcgccctgt acctccacgt catgggccat gtcggggagt actacgtcac
      721 cctcgcgccc tactacgaca aactgaccca tctcgtctcc tccgtcaccg tcgcgctcat
      781 cgccttcttt ctcgcggttc tcgccgagca ccagggggat ctccggctga cgaggaccgc
      841 gatcatcgtc ttcatcctgg tattcacgct ggcagcgggg gcggtatggg agatctacga
      901 gttcgtggcc gaccaggtct tcggcacgaa cctccagcac ggaaacatcg atacgatgtc
      961 ggacctcatc gtggacctcg ccggggcggt gatcgtggcg gttctcgctg cgattgccct
     1021 cgccaggaca gagggacacc agtttatccg gctcttcgcc gatccgaccc atcaccctga
     1081 accgggagat ctcgtgagcg atccgatcga tctggctcgc ggccgctgat ccgtccggtt
     1141 ctcgcccgcc gaatcatatt tatattcata gagaggaata aaatggagtg atcctgaagg
     1201 ctccaagcac acgatatctg gcgttattca tgcttatcct gctcccgtcc atgaccggct
     1261 acatggcctg ggaagggcgg gccgtcgagg tgaccgtcct tacgatcgac ggggcgcctg
     1321 aaggcatcgt ctatatcacc gacccgcacg tcagggcgtc caacatcgac cacgtccggg
     1381 aggtgatctc cgaggtcaac cgcttaaacc cctcgctcgt cctgatcggc ggcgacttcg
     1441 tcaccggtga ggagtcggac ttcgcctctc aggcggtatg gcggtcactg gacgcaccga
     1501 cctatgccgt gctcgggaac cacgattacc gggtgggcac cgacagccgg acgggcatcg
     1561 agagaacgct tgccgtccag acctcggccg acgtcaccgc cgccgggtac gacgtctccg
     1621 gcctgaacga cggttccgcc gacaccgcgt ttgccgacgg gctcgccgcg acgctggaag
     1681 agaacggggt gcatgtcctc aggaacgact acgccaaggt ctccgccggc gggaaggatc
     1741 ttgtcatcgt cggggtcgac gacgggtggg ccgggatggc ggacccgccc gacgtgccgg
     1801 cgacggacgc attcaccatc tacctcatcc acgaaccctc ctgcagggcc gactgggacg
     1861 ccgacctcat cctcgccggc cacacgcacg gcggccaatt cctcttcccg gtcatcaagc
     1921 agttaaacga ctatggcttc atcgagctcg cgggcatgtt cgacacgaac gggacgccga
     1981 cttacgtctc ccgcggggtc tgcagcgcga gcgtcgccgg ggtggacctc cggttcaact
     2041 gccggcccga gatcgtcctg atcaacccaa ccgaagaaca gctccggact atcggcggct
     2101 ctgcagcccc gctcccggag accggcgcgg tatgatcttt gctacatcgt ttgccctgct
     2161 ggggcttatc cttcccgaga gtgagcgggg ggccgctctc ggaacgaaca tcgccgccag
     2221 tttcggcggg ttcgcgctgg ggttcctggg gggtggcctc ttcacctatt acaccacctg
     2281 gcgggtgctg tttctggtgc ccctgcccgt cgccctgctc gccatcggga tcctccggcg
     2341 aaatttaccg ggggagtgcg ccctctcaag ggggacgcgc ccggatcttc cgggcatcgt
     2401 cctcagcacg acgatgatcc tcctgatcat ggtcggactc tccaccctgc caggggtgca
     2461 gggcaccgtc gcccttgtcg cccttgtcgc cggcctcgcg gcgctcgtcg ccttcgtcgt
     2521 ccatgaagcc cgggtaaaag acccgatcct tgatactcac tatttctccc gcaaccggcc
     2581 attcgttctt atcaacggtg cggtccgggt ctacctctgc ggggttgaag gggcagagcg
     2641 ggagggctcc ctccgcaagg ggggaggtgg aagcggagcc ccttcttcac tttctcaccg
     2701 cacaggtatt ctttatgccg gagggagtcc cagatgatcc tatcgtatgc tgcggaggta
     2761 ccagtaccgg ctctatccga cgaaggatca ggaacccctg atcgccaagc acctcgggtg
     2821 ctgccggtat gtgtacaact gggcgctcgc ccgcaagaac cgggcatatc acgatgacgg
     2881 caccagcctg tcaaagtacg acctcatgca gcaactcccg gctctcaaag aagagttgcc
     2941 gtggctgaaa gaggtcaacg cccagtccct gcagcaatcg atccacaatc tctcccgtgc
     3001 gttcacgaac ttcttcgagg gtcgcgccga agaaccgacc ttcaaaaaga agcacgaccc
     3061 cgaacaggcg ttcacggtgc cgcagtcgta tacggtggat ttcaagcaag ggacggtgaa
     3121 actcccgaag atcggtgtga tcaaaaccgt cttccaccgc accttcgtgg ggacgacgaa
     3181 gtctgcaacc gtcatccgca gatcgaccgg ccggtacttc atcagtatcg tggtcgagga
     3241 cggccggaag cctccgcaac ccgccgatcc gatcccggat cagacggtcg ggatcgatat
     3301 ggggctgaaa cactacgctg ttgtctcgac cggggagaaa gtggagaacc cgaagtatct
     3361 ccggaacgcc cagacgcggc tcgccgtctt acagcgacga ctcgaccgca agcagaaggg
     3421 ttcccggaac cggaacaaag cccggctcaa ggttgcccga tgtcaccaaa acattgccga
     3481 tcaacgaaag gacttccagc accagctctc ttctcgactg gtccgcgaaa accaagcact
     3541 ggcagtggag tctctgaacg tgaacggcat ggtgaggaac cacaaactgg cacgggcgag
     3601 cggcgatgcc ggatggggga cgttcctttc gatgctggcg tacaagtgtc gggaggcggg
     3661 gaaaaccctg ctgaagatcg gggtcttcga gccgtcttcc aagacctgtt cggtctgcgg
     3721 ctaccggaaa gccgatctga cactgaagga tcgggaatgg acgtgcccgg actgcggcac
     3781 gacccacgac cgggacctga acgccgcgat caatatcaag aacgttgcac ttgcaggcgt
     3841 ggataagcgc cgtcgagcct gtggactcgc tcccggtggg gagggggatg aagcaggaag
     3901 ccccctgcgt aagctggggg tagttcacac gatctcaacg aggctgacgg tgaacgtgag
     3961 gtcttcgccc acaagacgat ggttcgcgtc gatggtgacc gcgccgggcg atatatcggt
     4021 gaccgtcccg ggcagcacct gcccgcccgg cagggttatc ccgaccagtt ctccgacggt
     4081 caggttctcg gcgccggtga tgtcggccgg gtcgaggacg aagacgagat cctcgcggta
     4141 ttgcccgtac gcctggtcga ccggaacgtg gagggtcttt tcctctccca cctgcatccc
     4201 gacgacgccc gcgtcaaatc ccgggaccac cctgccgctg ccgacggtga acccgagcgg
     4261 ttcgcccccg acggaactgt caaaaaccgt cccgttctcg aaagtgccgg tataatggac
     4321 cagcacctga tccccggact tcacctgcgc ctgcccaccc tcgctcccgg tgcagcctgc
     4381 actaaaggcg aggatgcacg cgatcagcag caggatgcca tacgcatgcc tctccttcac
     4441 agttcaccct cgctatcgag cgcctttcca aagcactcca ctgcatcggc aaacctgccc
     4501 tgccggtcaa gcgctcttcc cttcaggtac catgcccggg catggcgggg gttggccgcg
     4561 aggaccttat cgaaacaggc ggccgcctga tcggatttgc ccaggtgccc aagggcgatc
     4621 cccttgcaga gccaggcatg ggaatccccg tcacccagct cgatcgccgt atcaaacgcc
     4681 tcgacggctt ctgcatgctt tcctaacgca tccatgagta ccccggtagc gttccagatg
     4741 gccgcgttct tcgggtcgac cgagagcgcc ttctcgtaac tcatcagggc gtcgtcgtac
     4801 ctgcccgcct tctcgagcac cgtcccccgc atgaagaggg cggcggggtt ctcgtcctgg
     4861 aggacgatga tcttgtcgat tgacttgacc gcctccccgt aacgcccgag gtggatgagg
     4921 gccgatgagc gggcatggag agtctcgatg ctggtcggtt cccggtccag cacctgcgtg
     4981 taagcggcga gggcatcctc gtatctgccg gccttctcat gggcctcgcc gagtcttcgg
     5041 agcgtgggag catccgccgg atcggcctcg atcaccttct cgaagcattc caccgcatcg
     5101 gcgtagcgcc cgagccacat gaagatctcc ccgcgccgct gccaggcagc ccggttctcc
     5161 gggctggcct cgatgatgcc gttggaacac tccagcgcct cttcatatcg ccccatatga
     5221 acaagagcgc tttcgagggt gtaccatgct tcgacgtcac gctcatcacc ctcgagcacc
     5281 agcgcgtact gtttgatagc ttcgccgaac ttcccgtccc gttcgagcgc catcccgaat
     5341 gccattcttg catcccggtc tttaggagag ttctccaggt agcgctcgta tgcttcgccc
     5401 gcctcttcgt gcctgccgac ggcttccagc atatccgccc ggattttggc aatcgcagca
     5461 ttcgaggcgt tggcggcgag cacccggtcg cagcactcga ttgccttctg gtgtctgccg
     5521 agccgtgcga gcgcaacagc gagtgcaaag caagcgccgg tatcttccgg ccggccgtcg
     5581 gtcacgtttg cgtaacactc cgctgcatcc gcgtactgtc ccatcctctc cagggcccgg
     5641 ccccggttaa accatgcaaa gaggtcaccc ggatcggtct cgacgacctg cttaaaacgc
     5701 ccggccgcct cctcataccg cccgagcgtg gcaagagcga tcccgaggtt caccaggtag
     5761 cccccggcat ccggatcggc tgcaaggagc gtttcgtagc agtcggccgc cgcagcatag
     5821 cgcccgagcc cctcgaggat ccatgccttc cggataagtg tcccgctgtc ccccgggcct
     5881 gcttccgtca cccggttgat ggatacgagc gcctcctcgt atcgcccgag gcgctcaagt
     5941 gccatgctcc tgccgaggag ggcgacacgg tcggccgggt cggcggaaag cgccccgtca
     6001 aagcaggcaa gcgctctggc gtactcaccg aggtgcatga acgcgtctcc cctgctcctc
     6061 tccgcttccc tatccccggg actgatcttg aggagggcat cgaacgcctc ggctgcctct
     6121 gcataccgcg agaggtgcag cagggcatct cccttcctgt gcaggagata gaggtcgtct
     6181 ccggaggttt cctgtatccg gtcgaggcac gccagtgcat cgccgtacct gccgaggttc
     6241 atgagagcat ccgccttcat cgagcgggcg acagcgttat acggctctct ctcaaggacc
     6301 gagtccagac agaggttggc ctcgtcgtac ctgccgagat ggagaagcgc gataccgctc
     6361 tgctgcagga cggagacgtt cccggggtcg acctcgataa catgctcgaa gtcctcctgg
     6421 gcctccgcat accgcccgag gtggagcaac gcaatccccc ggaagttata gacgatctcg
     6481 cttagaacat cgaacttcaa cggcttctgc ttccggggag tccccggctc gtccctctcg
     6541 aagagagcaa ggtcggagct gctcccgatc cacttcatcc cggtcatcgc ccggttctcg
     6601 agcacgcggt cgaacgaccg gactgcctcg gcgtactggc cgaggtggaa gagcgccatg
     6661 ccgcgggcat accatacctg gacctgaccc tggtcgtgtt tcaggatatg gtcgaaactc
     6721 ttgaccgccc gctcgtagcg gccgagcata tcgtagacca ttcccatcga gtagcgcacc
     6781 gggatgtggt cggggtcggc gagggcgacc ttatcgaagc actccagcgc ctccgcgtat
     6841 tttcccgccc ggaggagcag cgagccgcgg tgataccaga cggggatgtt cgccggctcg
     6901 gcggtggtga tcccggcgta gcagccggcg gcatcctgga accggccgag gtgctcgagc
     6961 gcccgggctc tgccgtagag cgcatcgagg ttgtgaggtt ccgtatcgag cacccggcca
     7021 aacgcgtcga cggcttcggg gtaatccccc cgatacagga gagcggtccc gattcttgcg
     7081 agcgcatcgg tgttgccggg gttcgccctg tggatcctct caaagcaggc cgccgcttcg
     7141 cggtaatcac cggtccgctc gagcatcctc ccgtgtttct cccggaggac aacatcctcc
     7201 gggtctccag cgagcgctcg ctcgtaagct gccgccgcgt cggcataccg gccgagcctc
     7261 tcaagcatct ctgcctgcca gtacgaggcg tccgtatctg cgggttttcc ttcgacgatc
     7321 ttctcgaagc atccggcagc ctcttcgtag tcgccggtca gggagaggac gtgtcccaga
     7381 gcccgcagtg tcccggcatc gccgggagac gactcgagag ccttcctcag ccaccccgcg
     7441 gcttcgtcgt tcttgccgag acagccaagc gcctggccca tgaagaaccg gacctgaccg
     7501 ttttcaggtt cgatcttgag cgccttctcg aaacttttca ccgcctcgct gtagttcccg
     7561 gcctcaaact gagagatccc ctgctttatt gaggggtttg cattcttccc ctgacccaga
     7621 atattaccga agaaatccat ccgacaccat ctgccgctgc tcgtttactc tccgattatt
     7681 caacgctgca ctctgtgaat gtcacccgtg gagaaggtaa ccgttgctga tctaagatta
     7741 atgcgccggc cactatttga aaagaccgat tttaagtaga gaaacgaatc aattataatg
     7801 ggtgctatgg attcgtaaac taccccccgc ttgcgcaggg gatcttcccg ctctgcccct
     7861 tcaaccccgc ggagataaaa gagtgccgct gcaaaaaccc tgcaccgtgc cggagtatcg
     7921 gatccccctg cagcctgtag ggggaactgc ccgttacgat gaaatctcag gaagataggc
     7981 tggttgggcc gctcctcccc cttcagacat ccaggatggg ttcgaactgc gacttgccga
     8041 ccggcccatg gaaactcgtg atctgcgggt ccatcccgca gaccgggcag acatagtcct
     8101 ccggcaggtc ttcgaatgcg gtgcccttcg gataaccgcg gagcggttcg ccgcgttttt
     8161 tgtcgtacac gtaaccgcag atcttgcaga cgtacttcgt cggctcccag agttcgaacc
     8221 cccacttccc gatctgcccc ttgccggtcg caccgcagac cgggcagacg tagtcctccg
     8281 gcaggtcttc aaacgtggtg cccgccggga caccgcggtg gggctcgccc cgcaacgggg
     8341 agtagatgta attgcagtac ttgcaccggt atcgcttcac tttctccatg cggatcacac
     8401 tcacctgatg gcaactaccc tgcatgaggg cagtttcacc tgataaggtt tcccatgatc
     8461 cgactctgca ggaagagggc tctcccggac agcaggatcg gctactgccg cctgccgccc
     8521 gtaaccgcgc gggagaggag cgagagtatg agcatgagga tgattgccat gccccagacg
     8581 accaggacca ctatgaaccc gagccacccg gccccgtgcg cgggcgcctg tgctctccag
     8641 gtgaaccccg gcgtccacgc cgtctgcccg ccgggacggt accgggaccg gtcgtgcgac
     8701 gcaaaaccgg caccgttccg gagcgcgagg tcatagcgcg cccgggtgtc ggggtcgatc
     8761 agcgtctcgt atgcctgctg gatggcgatg aaccgttcgc ctgcatccgg gtccgggttg
     8821 acgtccgggt ggtactgttt ggcgagcctc cggtaggccg cccggatctc gtcgggggtc
     8881 gcgtcagggg cgacgcccag gatatcgtag caggtctcca cagtgggcat cacacggtca
     8941 tatcgtatga tacctcgccc gtgtccccgt tcaccgtcac gcaggtgccg ggggcgaggc
     9001 gggagaactc cggcgggagg cggtcgacca tggggatccc cgcgatgatc gccccgaccg
     9061 cgatgatcgg ttcggcctcc gtattgatga tcgccgcagg ggccagaccg ttctgcttta
     9121 atgcgtagat aacgtaggac ccgaccgtcg accctttccc gtacgggaag gccagcacgc
     9181 gtccggcgat agattgcccc tcaagcggat gcccgcgctc cacgacggta ccggtctccg
     9241 ggtcgacacc ggagaggaac gataggggtt cggacgaaac gaggagctct ccgtttgcga
     9301 tacccctgga tatgcttctg ccttgcatga tcactgccac cacactataa tgtggttgga
     9361 gatccaacta tatgagtgac atggacgaaa ccattgccaa cactcctggc agcgagcacg
     9421 acatgctcac cctccagatt caggatctga aggcgcagat cctggattac aaacttaaaa
     9481 acaggttgct cgagaaggaa ctcctgcaac ttcggaaaga aaacggtcag ctcaaaaagg
     9541 tgccgctgtt tgttgccgct gtggtcgatg tacttgagaa tggcgaagtg tatctccggc
     9601 agcagggcaa caaccaggaa tacgtcaccg cggtcaacga gaaactccac cgcaccctca
     9661 agcccgggat gaaggtggcg gtgaacaata cgctctctat cgtcaagacg atcggcaaca
     9721 tcttcgattc gagagtcagg gtcatggagc ttgacgaaca gccaaacgtg acgttcgagc
     9781 aggtcggcgg tctgaaagaa gagatcgagg aggtcaggga agccgtcgag tatccgctca
     9841 cgaaacccga gatctacgag cgcataggtg tggagccccc gaaaggcatc ctcctctacg
     9901 ggccgcccgg cacgggaaag accctgattg caaaggcggt cgcccgccag tccagggcac
     9961 ggttcatcag gatgtccggg agcgaactcg tccacaagta catcggagag ggcgcgcagc
    10021 tcgtgcgtga actcttcgtc ctcgcccggg agcgcgctcc ggcgatcgtc ttcatcgacg
    10081 agatcgatgc gatcgggagt atgcgcacca acgacgggac ctccgggagc gccgaggtcc
    10141 agcggaccct gatgcagctt ctcgccgaga tggacgggtt cgggaaccgc ggcaacatcc
    10201 ggatcatggc ggcgaccaac cggatcgata tgctggatcc ggcccttctg cgccccggcc
    10261 ggttcgaccg gatcatcgcg gtcccgctcc ccgacgccgg agcacgcctc gagatcatga
    10321 agatccacac cgcgaagatg agtcttgccg cgaacgtgga cctccccggg gttgcagaac
    10381 tcgccgggaa caccaccggt gcggaactgc aggcggtctg ccgcgaggcc ggcatgatgg
    10441 cgatccggcg ggacgccgat gccgtcggcc aggaagactt cctcgcagcg atcaagaagg
    10501 tgaggcgcga ggcggcggca cccgatggcc gcatgtatac ctgatgggga ttcatccatc
    10561 cccgccgact tttcttcgca ccctgccgcc ggagtatgaa ggagggcatc ccgaaacccc
    10621 gttgccgaga gggatgttcg cggacctcct gactgcttac atctgcaccg cacagccttg
    10681 cgatggtgct cgaaatccac tctcgtccgc actcgaagcc ggatggtttt gtgctccacg
    10741 ttttcagaac gccacacccc gcacctggca gtgcttgaac cccagccttt cagcccgtcg
    10801 tattccgtcc ctcgcatgaa cgctgcacgg attcggaggg ggtatcgcca tagggggaac
    10861 gacgctccgc aggagcggag ttcgagcacc gaaggtgcgc ggaggggctg acggggaggt
    10921 ccctcccctg cccccacccc catggcgata gccaccgcgg tccagtgtac cgggtttttc
    10981 catagttgac cctcaatttc tctcaggata tactcccacg tcttcccacc gacggagtcc
    11041 agggtgacac cgaaggaagc cttatcgacc ttccacgccc acaactatgc aatgaacgtc
    11101 ctcctcattc agccggaggg ggttgacctc tacgccaccc tgctcaagtc cgataccagc
    11161 aggggggccc ttcgttttta ccagcccaat aggctgccgt atggtatacg catctggacg
    11221 gcgtcgctcg ggagtgcgct tgccctcgcc tccgaactcc ggtggtatat ccagcgatac
    11281 gtcgatgagg ttctcttcga gcccgaagag ggcatctact gctcgtccgg ccttgcccgg
    11341 cagatctacg agcgggatgt gaaacccgcg tcgccgtgga gctaccgctg cctctaccgg
    11401 atctcggggg accacatgat cgataccgtc tggatggacg agggcacgac gcccgaggca
    11461 tacgccgacc agaccgggac cggcgatacg atgctcgagg tctggtgcac gaaagaagag
    11521 tacccg
//
LOCUS       DAJB01000060            3343 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_136446, whole genome shotgun sequence.
ACCESSION   DAJB01000060 DAJB01000000
VERSION     DAJB01000060.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_136446"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          765 a          906 c          976 g          696 t
ORIGIN      
        1 attcctcccc cggttgaaac cgggggcctc cttgcccgtc gtatgtgaag gctttgaacc
       61 gtagacccga gaggagggat atcgtcctgg atggttctcc agatgatggc cctgttgatc
      121 ccgaaatagg catggatcaa tttgtcgcgc ataccggtca tctcgctcca gggaagagag
      181 ggacagttct ccctgacgcg tcggggaata cacttcgtcg cctcaccgat cacttccagc
      241 gctcgcgtga ctgcgtagac ggtcttgtcg tcctcgaaga actcctcata ggacatttcc
      301 cgggtgaaga ttgcaatctt ctcgacggcg tcaagaatat cgtccaggta atcaagggtg
      361 ttccgcggag ccgtcatacg tagataacct cattcaggat acggcggccg atgcgaggct
      421 tgagcgcgct cttttcgacc aggtccactt ttttgccgag aagttcggag agataccgct
      481 caagcctgag aaacccgaag atccccggca cctcggagaa ctcaaccagg atgtccacgt
      541 cgcttccctc gtgctcctcg ccgcgccggc acgagccgaa gatgccgatg gacccgacat
      601 gataggtctc ctccaggtac gccttcttcc cccggagaat gccgatgacc ctcgcgcact
      661 ccccgccggc ctcgcgggtg atggtgtcca tcctgatcgg gatatgggcc ttcccatctt
      721 gtatagatat ccccgccccg gtccgcagat gtggccaggc aggggccgcc ccgaaccatg
      781 ggcgttcccc gacggaaacc accaacagag cagcatactc gacacgctgt ccccgcggaa
      841 tgccgaatgc acattgcggc acatgtgcaa tttgtcccac acgatcccgt tatggacaag
      901 caggcgtgga agatgcaatt aatatagtgc ccggtaagac tctaccagag gaaagggaat
      961 gcaggttgcg agtgctcctg acggcaccat ctacattgat ggaaaggttg taaccgcgct
     1021 ggaccgtttt gtggcctcgg tgacggagat catcgagcgg tatacccgct acgtgatcat
     1081 cagcgggtac gttgcaatcc tttttggaag ggagcgcctg caggtccggg ggagcagtat
     1141 ggaattgagg tttgatcgcg agaagaaccg gcaggagcgg ctggcctttg tccggagaca
     1201 tgccctctgg gtcagggaag tcccaaacga ggtctggagc cgcgagcagg ccgatctgat
     1261 cgacgcgttc tttgaacatg cccaaaactt cgctctttcc cggtccgagt acctccggat
     1321 gcaccgggtt gcccggcacc gtcccgaacc ggtgcacggg taacttccgg ccgggcaaac
     1381 ggagggcggc cggctgcatg gtggagtgta tccattccgg atcgggatgt gcgcctccct
     1441 ctcgaatata gctgtcccgc gggcacgctc aaagaagagg aaacacccct gagagcagca
     1501 tcccgttgct ccagaacctt tttcacccat tggttcaaat atgttccacg tggaacagaa
     1561 tgtatccatc cagatcgttg ctctcctgct caggggcgac tcgcaccctc gcaaactcgc
     1621 aaaggaactg ggcatctcgc acactaccgt gctacggaaa cttaagggtc ttttagatgg
     1681 gaatgtcgtc gacttcagga tagagggaaa gaacagggtc tatttcttaa aaaaaaccat
     1741 cgaggcgcgg gttcatgcct atatggctga gtggtacgcg ctcggagacc tgatcgaaga
     1801 ggccccccat cttcggtcca ttgtcagggc ggttgcagag agagaggaca tcccgcttgc
     1861 cgtactcttc ggcagttatg cgaaggggac cgcggatcga aagagcgata tcgatctcta
     1921 cattgagacg gacgacagag aggtgaagcg agagctgcag cgagctcact tgcggctcag
     1981 cgtgaagatc ggaccctggg acccggagaa tctgctgatc caggagatcg taaagaacca
     2041 cgtcatcctg aaaggagtcg aacgttacta tgaaaagaca gcattttttg cgtaaactct
     2101 atcgtgaagg gaagatccag atcgttgagc ccagccttca gatccaggag gcatatcgga
     2161 agaagtcgga gagttatctc acctcggcaa aaattctgct cgaaaacaag cggcttgaag
     2221 agactgtatc gatggcatac tatagcatgt attacatggt gctggccctt ctcttcaaga
     2281 ccggcataaa atgcgagaac cattccggcg caatggtgct tcttgaacat ctgtatggtc
     2341 tcgacaattg ccggatcgtc gccgccaaaa aagaccgcat cgataagcaa tactacgtag
     2401 acttcgcgat cacggcagaa gaggtccatg actccataga ggaggcggaa gcgttctgtg
     2461 cagacctgta cgatttcatg gagcggttgc accagggcga tatctcacgt ctcagggagg
     2521 aagcagtgcg gctcctggat ggcccggacg agtgacctca tcgagggctg cggtctaccc
     2581 ccgcgtgttg ttccgggaga tgccgggcgg agatctcctg caaaccgttc acggagcgcc
     2641 tgcaggtcag ggaggagcca tatggaattg agatctgatc acgagaaaga ccggcaggag
     2701 aggctggcta ttataaatca ccgtgaatga acgtgcgata tggagtcggt aagagttctg
     2761 ctttttgttt atacggacat caagaacagt taagaacgca agcgcacgtt tgtccaccat
     2821 gaggcggaat gtcacaggtc taactccacg aactcgttcg tggccatgac ttcttcgata
     2881 tcatccgtga gccgctgttt ctttaccttc tccaccagcc tcgcgagagt ctcgtcgagt
     2941 gtctcgcccg ggtgcctgag atccaggatc tggttatagg tctcttccga tacggggatg
     3001 ttctccgcaa cagacattga atcttgatct gtgcggatca tggatatact ttcttcaacc
     3061 ggacgggagg ggtggagaag gcaggggtag agcggctggc cgcagggtac gatgtttcaa
     3121 cggaatgcga cgggccgaag ggccggagca ggagcacccc cggtgcgaac ggaacttgag
     3181 caccgaaggt gtgaacgtcc ggaacttgag aaacccaggg gttttgaggg cggagcggtg
     3241 aacagcgcga ggtgttcctc cccggttcac tccgggagga ggcgatcccg gagcatcgcc
     3301 cgaaccagat cccggtatcg gttcacgaca aaaaggtcgt cct
//
LOCUS       DAJB01000061           13279 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_17850, whole genome shotgun sequence.
ACCESSION   DAJB01000061 DAJB01000000
VERSION     DAJB01000061.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13279)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13279)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13279
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_17850"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2724 a         3942 c         4014 g         2599 t
ORIGIN      
        1 acaacgggca gaaaccctgt cccgaggact acctggtggg tgaccccgac ttcttcgggt
       61 ctggtttcta cctcccggga gggcaggtgt ccctgataaa ctgccgggcc gaggagaaca
      121 gtgcattcgg gttcctggtc ttcaacccgg gcggtatgct cctgtacaac tgtaccgaga
      181 gcggcaccgg gcgcgtcccg accaccggga cctctctaaa acccatctcg tactgtatcc
      241 tccagtccat gccggtcccg gcgaacccgt ccatcgtgat ggagcactgc cgaagtcttg
      301 attcgaacgg ttggggcctc ttcgtctgcg gcacggagaa cgccctcatc agggacttca
      361 tcctgaccga tccggcgggc atcgacggta tcggggcagt actggggtgc tcccccacgg
      421 aactcccggt gaactcgacg atcgatgcgg gcatctccaa ctcgaccatc gatcttgtcg
      481 catcgggaga ccggccacag acgctggtgt acatcgtgaa taacaggaac gtcacgtact
      541 ccgggcaggt cttctccgat gctgcccggc cggtggtggt cgagtggacc cgatcaggag
      601 atccggacct gaccggtctt gcggtgctgc cggccaacag cacggcctgg tgattcgggc
      661 ggctgcaccg cgatcgggga tattctggct cccctgctcc tgaaagcatc ttctgtgata
      721 ttgtcgatcg atcgagggca gaggctattc ctgtttggtt ccgggacctc tgatccggac
      781 cccccggcag gagcgggccg actaaatcct taaatggtac ctctgcacca taagggtatt
      841 aacctggcag gaaccaccac ggatcgtcgc gatctgctcc aggcgtccgg tttgtccctc
      901 tggcggggga tcatcggatg attcgttcaa cccgggacgg tgaactctcc cgatcgccga
      961 tacacccggc aggatggttc cgcagaacac cggtcgagga aataacagac aggtcatagt
     1021 cccatgtcag cacgaccaca actgggaccc ggaggcagac gcatcctcat cgccatcgca
     1081 gtcatactga cggtgatcgg cgtcgtcgtg ctggtcatgc acttttatcc gggcccggtg
     1141 cccccgcctc ctcctccccc accgatcacc cccattccca cgacaccggc agtgcccccg
     1201 gagatctccg gaccgacggt cgtcgtcgcg gcaagcgata gcagtcgcgc gtcgaaaacc
     1261 cgggctgaca tcctgtgcga cggtaccgat gatcaacgcg agatccagga ggcattcgat
     1321 gcactctcgt cgtcgggtgg cacggtcgcc ctgacggaag gcacgttcaa ttgcgctgga
     1381 agcatctacc cccgcgcaaa ttccatgctc cggggggagg gaccgaatgc gacattcctc
     1441 gagttcagca agaacgggag gctccatgtc tcccgggaat ccgtcacgct ctacaacttc
     1501 cacgtcaggg gcaccggata cccgaaggtc gagcccgacc tctggctcgg cgtggtcacc
     1561 atctatgcga gccacgcgaa ggtcctggac gtcgcgggga ccgccgatgc aaccacccag
     1621 gcggtcttcc tcctgctgca cgacccggac gtctatgccc ccaccctgga agacatcgag
     1681 ttcgtcaact gccgggccgt ggacaccggg acgtatgggt tcctgcacaa cgcctggggg
     1741 acagagaaca cggtgatccg ggatgtccgc tacgagaact gcgccgcaat caactgcggc
     1801 aggtatggag cgttcaaccc ctgggtgacc gggtttgact ttgcggagtt aaacgacatc
     1861 gagggtctcc gcgtgacgaa ctgcctcgca gagggcaacc tggagtccgg gttccacttc
     1921 gagtgggacc ccgagaagcg ggactgcatc ctcaagaact gcatcagcaa ggataacggg
     1981 cagaaagcct acccgacgaa ggcctacctg gaggacgata tgtccaccca ctacttcggg
     2041 tgcggctact acgccccccg gggtgacatc accttcatca gctgttactc tgaggggaac
     2101 agccgccacg ggttctatgc caccaacggc ggcaagctct acagttgcgt cgaccaaaac
     2161 gtcggtgccg ggaagaccga ttaccggatc gtccagccgg catcgttcta cgcggccccg
     2221 acccgatcga taacgccgtc gctggtgctc gagaactgtt caagcatcga ttcgcacggc
     2281 tacggcctcc acatcgacct cgcaagcgat gttctgatca agaacttccg cctggagaac
     2341 ccggccggga tcgacggcaa ggcgacaaac ctcggaggat cgcagggggg accgctggcc
     2401 aactctgtag tgaacatcta tgcgtcgggt aaccgggctg aaacgctgat atgggccagg
     2461 aataacgaga atgtcgagta ctcggggcag attgtctcgg acacggcgaa gccgtttgtg
     2521 atcgagggcg ttcgcaccag ggatgtccgg atcaaggata tggagatcgt ctccgcaagc
     2581 ctcgcgccgt tcaccaacgg tgtcatcctc gcaaatacgg tgccttccgg ggcggtaacg
     2641 tttgagaatg tggcggtcac ctcgggggtg cggtgaccgc ccgtgggcgt gaagcatggt
     2701 cccctctctc cgtccgatga cggcgcctgc atggtcccgg catcccggtg accaacccag
     2761 gtcgacaacg ttttgctgga tccggctata ttctggtact gaatggcgtt ctccggccga
     2821 tcctgtgcga gggggttatt gcgcggaggc gatgttgcag gcattctccc ggcctttccg
     2881 gggtgcggca gggggtggac tgccgcctgg tcgatcgcag atggcgatcc cggatatttc
     2941 agggatttat acgctttgca gaataatatc cctgattcag ggtctgtttg cccgtccgga
     3001 ctgtcaaccg cgatctgcga tcaagggggg tattctccgc gggatctgcg ctaaagtttg
     3061 gtgcaatatt tatatattcc tatttttctg taagttttta aaatagttga aattaacaac
     3121 tttgactgta aataactgcc cgtttgtaat tttaaataat ctgtattgcc caatcaattt
     3181 aaaaagcctt ttaaattact gttgtcagat aatgcagtat ctcccaagag gtgagataat
     3241 gcgagataaa cggacaacga agcttctaat cgcagtgttc ctgatcgtcg ggttgatccc
     3301 gatcgtcgga gcactcgagg cgacccccaa cacaattgtc gcggcaagcg acagcagtgc
     3361 ggccgataaa gcatcggctg cctacgtctg tgacggggtc aacgatcatg tggagattca
     3421 ggcagcactg aacgccctgc catcatccgg cggtgttgtc ctcctcacca gcggaacata
     3481 caactgtgcg ggaatcattg ctcccagagc aagctccact ctcatggggg aaggtgaatc
     3541 gacaacattc ctgaagttca ccaacgatgg acgcatcaac gtcgaccgtg agtacattac
     3601 ggtggacggg ttccacattc agggtagtgg ctacagcagt ggtgtcaagt ggctcggtgt
     3661 aatgaacatc cgggcaagcc acgcgaagat ccacaacata acgggtaccg cggatgcaag
     3721 cattcaggcg gtttacctcc tggtccatga tccgactgtc tacgccccca ccctcgagga
     3781 tgtcgagttc atcaactgcc aggccgtgga caccgggacc tacgggttcc tgcacaacgc
     3841 ctggggctcg acgaacatgg tgatcaagaa cgtccgctac gacaactgcg ctgcaatcaa
     3901 ctgcggcaag tacgggcagt tcaatccctg gatcaccggg ttcgactttg cggaactcaa
     3961 cgacatggac ggcctccgcg tgacgaactg cctcgctgag ggcactaccg agtccgggtt
     4021 ccacttcgag ttcgacccga aagtgacgaa tgccgtcctc gagaactgcg tgagcagaaa
     4081 caacgggcag aaagccttcc cgacgaaggc ctacaacgcg aacgacatgt ccacccacta
     4141 cttcgggtgc ggctactacg ccccgaactg tgacgtgacg ttcatcaact gcacggcgga
     4201 gggcaactcc atgaacgggt tctacacgac gaacggtggc aagctctaca actgcgtcga
     4261 gaaggacacc ggcatcggga ggacagactt ctcctaccgg gtgcccgccg gctactacag
     4321 cgtcccgagc cgctcggtca acccctccac ggtgatggag aactgcacga gcatcaactc
     4381 gcgtgccttc ggcctccagg tcgacctcgc gaacaacgtc aagatccgga acttccacct
     4441 gatcgatccg gtcgggtaca acggcaaggg ctcgagtctc ggcggcacga acgggcagtt
     4501 cgataactcc gaagtgagca tctatgcatc cggcaaccgc gtggagacgc tcgtatgggc
     4561 gaaggagaac gtgaataccg tcttctccgg tcagatcgtc tccgatgcgg caaagccgtt
     4621 cgtgatcgag ggttacagaa cccagaacgt cctcgtgaag gatatggaga tcgtctccag
     4681 gaccctcccc gccgggtcct ccggtgtgac catcgtaagc acggttccgg ccggtcaggc
     4741 gaccctccag aacgtgaaag tgacgtccac cggatcgccg gcacctaccc cgaccgttcc
     4801 ggtcactccg gtgcccacca caccggcttc ctccgggaaa ccggacctcg tggtgactga
     4861 tgtctcctgg gaaccggcaa acccggttac cgggagtgcg gtgaccctga aggcaaccat
     4921 caagaaccag ggagatgccc ccacgcctgc gggcgcgacg cacggtgtca tcgtcacgtt
     4981 cgatgacggt gctgccgggc cgggtgtctg gtcagatacc tacaccagat cgatcgctcc
     5041 gggtgcatgg gtcacggtga ccgcgaacgg cggctcagcc ggtgcaacct ggaaagccgt
     5101 cgagggcact cacacggtga aggccaccgt ggacgacgtt aaccggatcg ccgagagcaa
     5161 cgaggcgaac aacgtccgaa ctgaacagat aacggtctca aagaccgcgt cggggtcaac
     5221 cccgaccccc actcctacca ctccggctcc ctccgggaag cctgaccttg tggtgaccgg
     5281 catctcctgg gaaccggcaa acccggctac cgggagcccg gtgactctct cggccacgat
     5341 caagaaccag gggacggccc ccacgcctgc gggcacaaag cacggcgtcc tctttacatt
     5401 cgatgacgga gccgccgggt ccggcgtctg gtccgacacc cacaccacgg ctatcgccgc
     5461 aggtgcatcc gttacgctga ccgcgagcgg cgggtcggcc ggtgcaacct ggaaagccat
     5521 cgagggcact cacaccgtga aggccaatgt ggacgatgtc aaccggatcg ccgagagcga
     5581 tgagaacaac aacgttatga gcaagcagat cgtcgccgga accatccggg ttcccgtcag
     5641 gggagacctc aacgaagatg gcaaagtcga ctgggccgac gtgacgatcg ccgccgggat
     5701 ggcacaggga accactccgt ccaatgccgc tgctgattgt aatggagacg gcacagtgga
     5761 ctggaaggat gttgccctgc tcgccgattt cttcttcggc aggatctctt ctctgtaaag
     5821 cgtttgccag gagcagtggt tttgtccctc ccctcctcct ggttttgcca caggtgccgc
     5881 cccctaacac ctgtgcagtg tgtaaccata ttggttatgg gcgtttctcc tcaccagatc
     5941 cggtgagcat cttctgctgc cgtcttgtgc aacgaggccc tgaaaattct ctcctgaagg
     6001 atctccggcc ttgagggggt cggagcggtg gatacatcta tataggtgcc atccgatact
     6061 ggcgaccgat ggcagtagca ctccccaaaa acttcagtga ggtcaggcag cacttacagg
     6121 agccgcttat ccggaattca ttctatatca tggcgtcatc gttcgtcgct gcggggtttg
     6181 ggttcttctt ctggatgatt gcagcccggt tctactcgca ggcggacgtg ggtattgcaa
     6241 ccgccctgat gtcgtccatg gggctcctca tcctcatctc ccggctcggc ctggaccagt
     6301 cggtgatccg gttctttcct ctgcgcgaca agaacaaggt tctcgggacc gccatcctcg
     6361 tgccgacggc agtggcgctt ggtgcggggg tgatcttcat cgccgcagtg gacgtcgtat
     6421 ctcccgagct cacgatcgtc aggacggtcg cacccctcta cctcatcttc ctcggggcct
     6481 actctatcac ctgggttctc gagggcgcgt tcaatgcact gcggaagtct gaacactact
     6541 tcgccctgaa cctgctctac ggaacaagga tcctcttcct ggttcccctg gtcttcctcg
     6601 gagcaatggg catcttcagc gccttcggtc tctcgttcgt cctcgggctc gtcctcgccc
     6661 tcgtcctcct cgcccggtgc tccgtgcggc cggcactggg tgtggaccgg gtcttcctgc
     6721 gggaggcatg gcagttctct gccggcgcat acatcgccgg gatactgatg tccgcgccga
     6781 acatggtcat cccgatcatg gtgctccacg tgctcggggc ggagagcacc gccaattatt
     6841 acatcaccta cgcaatcgtc tcgatactct tcatgatccc ctacgcgttc acgacctcgc
     6901 tctttgtcga gggaagccac gggggggaga tgaagaagag cgtgctccgg acgctcgcga
     6961 gcatgtttgc cctgctcgta cctgcaatca tcgcgctctc tctcttcggg gagcagatcc
     7021 tcaacctgat cggcaaggac tacgtcgaag ggatggccct gctccaggtg ctcgccgcgt
     7081 cagccctgtt cgtctccgtc tgccagacct tcatatcgat tgcaaaggtc agaaacgata
     7141 tccggagcct catcatcctc agcagtttca tcagcgttgc cctcctcggg ctcggctaca
     7201 ccctgatgaa ccggttcggc ctcatcggca tggggtatgc gtggctcacc acgtatgtgg
     7261 caggaacgct ccttgtcggg ataatcctga agaagaaagg gtggatctaa ctgttctccg
     7321 ggctgctgca tacccgggag gagttccgtc tccggtcatg cgggggccgc cgtccgctct
     7381 tcaccggtag tagatggtgg agccggcatt atcgtagacc ttatgctggt tctggatgaa
     7441 ccgccctgca tcaacgtagt ctgccgtggt gaccgcgtac tcctgcgcct cgatccggac
     7501 actatccttt gcgaggttat acatgccgaa gtagaggtac gagttcggct ccagcagcga
     7561 cgcatttgcc gggatgtatc gctgacggcg cgggtcgaat ccctggagga gccaccagcg
     7621 tgtgccgtcg acgtagatgg ggcgctcgct cccttccgag aagagccacc tcgccccctg
     7681 cacctctttg ccgttgaaga ccggtttgtc tcccgtcgtc tcgagtgcca tcgatgtcgg
     7741 gctgtcgtcc accacctgat agatgagccc gctgttgaag aggaagaaca cgaccaggaa
     7801 tgccgataat ggcttatagg ctgttcccat gaacggggcg gccgtgatcg cacgtatcct
     7861 ccccaccaac gcctcgtaga gtgctatgcc gccgatgatt gcaaacgggg caaggaagat
     7921 cagggtgatg tggtagagcc ggctggtgtt gagcgagctt gcgaagaacg ggacgacgat
     7981 gccggctacg ttgatgagga ggaagacgag cgaaatggcg aggtactcgg gctcgatgcg
     8041 ccaccgctcg cgcctcgcca gggttgcaag cagtcccacg gcgatcagtc cctgggtggc
     8101 gatatggaca agctttgcca ggccgtgcag gggcgtgacg gcctggctgg tgaggatatg
     8161 catcccctgg gcggctatcg ggctgaatga gtccgcaaag agggtgctcc agatcctgtc
     8221 cccgatgccg gtgaccgtag caagcgccgt gccttcggct accatcgagt accagagata
     8281 gacgagggcg gcaaagatga ggatgtaagg gaggacgagt gtcctgaccg gcaccccccc
     8341 gccgccggtt cccgatggag cgagcgggtc ggacttcagg aaacccgatg ttccggtgag
     8401 cctctccggc agccagggtc ccaggcgctc ggggatgacg agcagcagcc atgccggaac
     8461 aagcgagaag aagtagatgt atgagaggcc gtaatgcgag acgatcatgg caaagccgaa
     8521 ggtgaccatg agaaacgcct gtctgccccg ggccatggac ttatcgatca tcgcaaggat
     8581 ggtcagtgtc aggaagagtt ctgcgatctg ctggcgggcg agggagatca tctctgtata
     8641 gaaggtgaag aacgcaaaga agaagaagat cgagagaaac ccgatcctgg cgtccgtctg
     8701 tttctcgacc gcccggtaga gcccgagcgg cacaagcgcg aagaggaggg ggtagaggat
     8761 cttgaagacc cagatggggt ccagatcgca gatgagcgag tagatgggga cgagcgcaac
     8821 gacggagagc attccgtttg tgttatatgg catgctcatg tcccagaggc cgggtatcag
     8881 cacgctattc acgaggtaga gctcgtggtg aatatcatag ccccagatat actccgatat
     8941 cagcgaggta tggtagagga gcgagagcgc gatagtgtag atcgcgagcc catagcagga
     9001 tgccgggatg agacggtcga acccgacagc gaggacgatg atggcgatca ggacgaggag
     9061 caggaagagg aacgtgacca tgtggtactc gttgcgcacg tatgtcccga cgatcgcaaa
     9121 gaacgggagc agcatcagga acggtgctgc aggggaggag ggaatggcta actccgggct
     9181 ggaggcggca tgtccacggt ccctgatgag agcgaggtag agcagaacct gcatgacggc
     9241 gacgattgta aggaagagca tcacaaacga gaacggcctt gaatacccga gcagggggta
     9301 ggtgatgttg gcggcgaggc cggtgagcat gaggaacgca aggctcagcc cgacggagta
     9361 cagcatcgtc tcgattgtgc cgcgatcgtg gagcctgagc gccttcagca ccaggactcc
     9421 gggcaggaag gtgagataga gaaaggccag ggcctctctt gcgatcggta tatagtatcc
     9481 gaagaggccg aggcagacta ccgcgacgaa agcgagctgg aatgcctgaa atgcccagaa
     9541 gaacgttttg ttatcccagt cgttcatctg gaacgggttg agcaggtgca tggtatccct
     9601 tgaacgtctc tattcggcta tgtgcatgga cagggtgtct tcgatggcac ctgacgggcc
     9661 gtacgccgca tcaatgcacc tactctccgg tccacctatt aatgtgttgt gcaggcccgg
     9721 ccttgttgtg ggagcggggt tccgttcccg cgcatctgct ccggcccggg aggtgccgac
     9781 ggtcatcgct tgttcccgct ggcggggact gtctcggcga aggtctcgtc tgcagggggc
     9841 ttttccctcc cctgagcagg tgcaagttcg ttgtagaccg cgaggagcga ccgtgcgatc
     9901 gtttcccagg ccatctcggt cttcagtttc atgtaaccct gctctcccat gcgccggcag
     9961 gccgccgggt ccttgagcag gctcacgatc gcggctgcca atgccgcagg atcgcgtggg
    10021 ggcacgatgt acccggtcct ggcgtcatcg acgacctccg gcaggctccc cacgttcgtc
    10081 accaccaccg gccgccggaa cccgtatgcg gttggtatca cgccgctctg ggatgcttcg
    10141 aggtacggaa gcgccacgac actcgcctgc tggaagaggc gggcgccctc ttcgtaggat
    10201 atcctgtagt tgtagacctc gaatgcatcg cggcccgcca tcgcctcctg gtacctgccg
    10261 aagtcctccc ccgttccggc gatgacgatc cgggcgtccg ggacttcccg ggtgatgagc
    10321 ggttctgcct ggatcaggca gtcgagcccc ttgtagcggt ggatgcggcc gaagaagagg
    10381 acccggctgc cgtccggttc caggccctcc tgttcgaact tcacaaatgg cgccacctcg
    10441 tgctcgccga tcgggatgac atgtactttc cagcccggga tgcggtggtt ctccaccagg
    10501 atctccctga gcgctttccc gtggacgaag atccggtcgg gcaggacccg tgacatgaag
    10561 agggtaaggc ggtagagcag ggagccgata tccaggaggc ggtcttcgcc ggggtgcggc
    10621 tcgatgtcgt ggaacgtcgc caccagcgga tacttccgga gcatggggag gagcgggcag
    10681 agcatggggt tgttcacctg gaaatggatg acgtcgggct cgaagtcgtt gactgcagtg
    10741 acgatctcct tgagcagcgg gtagcacgac ggaccgagcc tcccggcgta ccgggagtag
    10801 ccgaagatat ggaccttcac cttcgggtcg atgccctcca ggtactcctc cgacctgtag
    10861 tccggcagca gcagcagcac ctcggtgtgc cgggcaagtt cgtttgccat ctggatggtg
    10921 tagtcgtaga accagaaggt caggcatgcc actcgcatag gggcactctc tgtggagacg
    10981 ctctatatag acattgcgtc cgggagggcc gttaggccca atgggcccgg gtagatgggt
    11041 atgagcccgc cggattccgc tccggcggcg gctctcctcc gactgttttt gcccggcctc
    11101 cctgccggat tcggtccctg tgacccccgc tgctgtccag cgggctggtg cctgtccccc
    11161 ctcacgccac cgcccggcaa cgaggaccgg agcccccggg ccataccggc gcacggaacc
    11221 gaatttccat ccgttctgcg tgaaagcgtt tcgagaaggg ttgctcccgg cgatcagcga
    11281 cagaaggcgc ggctatttat gtcctttggt tttagtttca ataatcatat aatatctttt
    11341 tgcatcaacc gatctccgct gtcctacacc ttattccgaa ctaaacggga tatggctgcc
    11401 catattgtcg aataaccctc ttatgcaatt ttattttatg aataaaatat atatatggtc
    11461 atagtaagac ggtagtaata cctcacagag aggctgaatg gcatgataga caggcaaaat
    11521 attctacgac tggctgcagt acttctggtg tgctgcctca tcccggcatt ctccccggta
    11581 agtgcagccg agtctacaag tccggtcacg ctcaccgtgg ctgcaaacga tagcaccgaa
    11641 ctgtcgaaga acccggcaga ctatgtctgt gacggcgttg acgaccaggc cgagatccag
    11701 gcagcgctcg ccgcactgcc ggaaggcggt atggtcgttc tgaccgaggg tacgttcaac
    11761 tgtgccagag tcatcgcacc ggcggcaggc acgacactct ccggccaggg tccggaagca
    11821 acgttccttg aattcaccca cgacgggcgc atcagcgtcg atcaggagtc cattacgctg
    11881 gacgggttcc acatcaaggg aaccggctac agcagcggcg tcaagtggct cggcgtgatg
    11941 accgtccgtg cgagccacgc gaagatccac aacgttgagg gaacggcgga tacgagcatt
    12001 caggcagtct atctcctgat tcacgatccg gcggtctacg cgccgaccct gcaggacgtc
    12061 gaattcgtca actgcaaggc cgtggatacc gggacgtacg ggttcctcca caacgcctgg
    12121 ggttcgtcca acaaggtgat caaggacgtc cgctacgaga actgcgccgc gatcaactgc
    12181 gggagcgaag gtgcgttcaa cccctggatc accgggttcg actttgcgga actcaacgat
    12241 atggaaggcc tccgggtgac ggactgcctc gcggaaggta acctggagtc cgggttccac
    12301 ttcgagttcg accccacggt gacggacgcg gtcctcgata actgcgtgag ccggaacaac
    12361 gggcagaaac cctacccgac ggtggcctac aatcagaatg atatgtcgac gcactacttc
    12421 gggtgcggct actacgcccc gaactgcgac gtgacgttca agaactgcac cgcggaaggt
    12481 aactccctgt atgggttcta cgcgacgaac ggcggcaaac tctacgactg tgtcgacaag
    12541 gacaccggcg ccgggaagac cgactacacc taccggaaac ctgccggcta ctacagtatc
    12601 ccgagccgct ccgtgaaccc cgccctggtg ctggagaact gctcgagcac cgactcgcac
    12661 ggctacggcc tccaggtcga tctcgcgagc aacatccaga tcaggaactt cgagctcgtg
    12721 aacccggccg ggatcgacgg taagggtgca agcctcggcg gcacgaacgg tcagttcagc
    12781 aacgccgaag tggacatcca cgcctccggg gacctggttg acacgctcgt atgggcgaag
    12841 aacaaccaga acgtccagta ctcgggagag ataacctccg gctccgaaaa ggcgttcctg
    12901 gttgagggcg gagagaatgt ccggacagag ggcatgacgg ttgcgcctgc cggcgagtaa
    12961 accggtcgat attatcgggc ccggtcccaa tcttttttgg ctttttgggc cttgttgaaa
    13021 tgctttccac aacctctgga taacacccgg ttaccaatag gggtcggtga cgtatggatg
    13081 tctggagcgt gagcatcgca gatgtgggag gcacggcaaa cagagtaatc aaaacgggaa
    13141 aaatatcacg atgaagtgga ccgtggggac tgcgcacctt cgcacctggt gcatcttgtc
    13201 agtttatgtt aggaattttg gcgtgtgggg gcgggcgcta tggtcgcact ccgggggtac
    13261 tccggagcac ggctttcca
//
LOCUS       DAJB01000062           16873 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_57075, whole genome shotgun sequence.
ACCESSION   DAJB01000062 DAJB01000000
VERSION     DAJB01000062.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16873)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16873)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16873
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_57075"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3028 a         5430 c         5190 g         3223 t
ORIGIN      
        1 gacaacaatg cgctttatgg cattcatggc attcgtgata tatgtactac tgacagaaaa
       61 acattgccgg tgtatcgggt attctccggc aagaatcctt caactgggag gtgcgactcc
      121 cggtgggaag ccgtgtcagc aattcgacac tgtataatgg caattccttc gaaaatctgc
      181 gtgagaatgc tctacatcag gtggatcttc gatcgctgga cactttcggg acgacttcaa
      241 tgattattct cataataatt atagatataa tcattatggc gccgccggga gagacaccct
      301 tatctcctga gagcgacgat cccctgccat ggtacggtcg gggatacccg cggatcgcct
      361 ctccggggtg acgggccggt gatcgaggag cacctccggg cagggacgaa cggggcgctc
      421 acgcgagtcg cggccctcat cgcccgtgcg ggggcgaccc cgaacaccct caccctgctc
      481 ggatttttcg ggatggcggt tgcgggggcc ctctgcgccg gaggatcgtt ctttgccgcc
      541 ggaatcacgg ttgccgcatc gtgcatcttc gacgcactcg acggggcgct cgcccgcgtg
      601 accggtgccg cttcggtctt cggcgcgttc ttcgactcgt tcctggaccg ttacgcggag
      661 gcggcggtct acgccggact cctcgtccac tatgcggggg cggggacggc ctggggcgtc
      721 gaggccgcgt ttgccgccgc gatcgggtcg ctgatggtca gttacgccag ggcccgggcc
      781 gaaggacttg gaatagcgtg ccgggccggc ctcttcgccc ggcctgagcg gatcgcggtc
      841 atcatcgtcg ggctgctcac cgggttcgtt ctcccggcgc tcgtcatcct cgcggtcgcc
      901 acgaacgcca cggcaattcg gcgacttttc tgtgtctggg gggctacgct ctccccgtca
      961 cggcctcccc ggtcgccgtg aacccatctg ttgcgccgac gatccggtag ttcccggcgg
     1021 tcttcacgtc gtaggggctg ccagtcgtgg agtacgggat gatgaaggtc ccgttctcac
     1081 tctcctgccg gtagacgaac tcccgcccgg tgttggtgac aaccgtgacc tcgatgacac
     1141 cctcgcccgg tcccgtgcag tcatcgaccg ccgccggcgg cctcgtcggg ttcggcatcc
     1201 tccgggaccg ggccggcacc gggttcggtc cgggcactgg ccctcttcct caccgggatg
     1261 ctggtcgtct tctccggcat cttcacgagc atgatgctct taaaatggag ggaggggcag
     1321 gatgctccgt cccgcgatcc tgatttttag gcctctgttt tgtttttctg acccgtaaac
     1381 gggagatcga tcctgttata tatggttggc ggtacagttg tgaaccggag gaactgcaaa
     1441 tgaccgtttt atcaaacaat caacagaaga tagcaaggag gatcgatgct ggcaccgccg
     1501 tcgaagtctg gcgcgactgg aagtggcagg tgcggcacgc gatcaccgat atatccacgt
     1561 tcgagaggct gctcggcata tcgttcggag aagacgaacg gaaggcgctg gaggagaccg
     1621 taagcaggtt ccccctgcgg ataaccccgt attacctctc gctcatcgac gcgaaagata
     1681 tctggaacga cccgatcttc atgcagtgct tcccgtcgcc ggcggagctg caggtcgagc
     1741 ccgacgatct ggcggacccg ctcgccgagg acgccgacag ccccgccccc tgcatcaccc
     1801 accgctaccc cgaccgggtg ctcttcctgg tcagcaacgt ctgcgccatg tactgccggc
     1861 actgcacccg gaagcggaag gtcggggacg tcgattccat cccgtcggag accgagatcc
     1921 tcgagagcct cgactacatc cgggaaaacc cccagatccg ggacgtcctc ctctccgggg
     1981 gcgacccgtt catgctcccc gacgactacc tcgactggat cctgaccgaa ctcgacgaca
     2041 tcgagcacgt cgaggtggtc cggatcggga cccgggtccc ggtcgtcctc ccctaccggg
     2101 tcacggagga cctcgtcgcg atgcttgccc gccaccaccc gctctgggtg aacacccact
     2161 tcaaccaccc ggtggagatc accacatcgt cgcagaaagc gcttgcacgg cttgccgatg
     2221 ccggcatacc gctcggcaac cagaccgtcc tcctggcggg ggtgaacgac tgtccgcgga
     2281 tcatgaagac tctggtgcag aaactcgtcc ggaaccgggt ccgcccctac tacctctacc
     2341 agtgcgacct ctccgagggg ctcgcccact tccgcacccc ggtctccaag gggatagaga
     2401 tcatcgagag cctcatcggg cataccagtg ggtttgcggt cccgacctac gtcatcgacg
     2461 ctcccggggg cggcgggaag atcccggtga tgccccagta tctcatctcc tgggccacga
     2521 accgcgtggt gctgcggaac ttcgaaggcg tgatcaccac ctaccgggag cccgactcct
     2581 acgagccggt ctggtgcgac cgcaagtgcg cgacctgcag gcagtacctc aggctcgagg
     2641 gcgcggatga gtccaaagcg gtcgggatcg tcaaactcct ctcgggcgag gacccgacga
     2701 ccgcgctcgt cccggagcac acggagcggt tcgagcggag aaatgacaac tgataccgtc
     2761 gcgaccatcg gcagcaccct cgtccagcac ggccagctga gcgaccgcat ctacgtgatg
     2821 cacctctcgc cccgtgacct tcccggcatc ctcgacgacc tcgacgggct cgcacagcgc
     2881 gagggctact ccaaggtatt tgcaaaagtg cccgcttccg ccctccccct cttcatcacc
     2941 cggggctaca tcgtcgaggc acgggttccc cggttcttcc ggggcaggga ggagggttgt
     3001 ttcgcggcga agttctttga tgcggaacgc cagagagatt cggccgatac agccgcggtc
     3061 ctcgcggccg tccgggcaaa ggccgacgaa cgacggccgg ccgtcctgcc gccggattat
     3121 atgtgtgccc tcgcaacaga agacgatgtc gacgaccttg ccgcgctcta ccgggaggtc
     3181 tttgcgacct atccgttccc catcggcgac cccggctacc tgcgggaggc gatggccggg
     3241 gactaccgct acttcatcgt ccgcgccgcc gacggccggc ttgccgcggc ctcctcggcg
     3301 gagatctacc ggcaggacga gaacgtcgag atgaccgact tcgccgtcca cccggatttc
     3361 cggggccgga gcctctcggg cttccttctc tcccggatgg aggaggagat gcgggcggcc
     3421 gggatgaaga ccgccttcac catcgcccgg gcgctctcgt atccgatcaa cgccacgttc
     3481 gcctgcgccg ggtatgcctg ggccgggacc ctcgtcaaca acaccaacat ctgcggcggt
     3541 ttcgagagca tgaacgtctg gtaccggccg ctggacgggt aaaaaagagt atcccggtgt
     3601 ttacgcggag cccttccgct ccgcatcctt ctttttgtag ccctcaagca ggatcgtcgt
     3661 caggttcttc acgggcgtgt ccgtacagtc ggcgatgtgg aactcgcaga acgggcagac
     3721 cgtcaccacc acgtccgcgc cggtcgcctt gatgcactca tcgcgctttg cgccgagcgc
     3781 ctttgcctcc tccggcaccc ccgaccggac gccgccgccg gcgccgcagc actgcgtcgg
     3841 catctcgatg aattccctga cccccaggcg caggagttcc cgcggctgct tgctgatccc
     3901 ctggccccgg aggaggtggc aggggtcgtg gtaggtcgcc ctgacgtcga gtttcgccgg
     3961 gagctcgatc ccgtactccg tcaggagctc ggtgacgtct ttgacctcga acggggtgtc
     4021 gtaatcgttc ttcagcgtcg ccccgcaccc ggcgcagatc gtcatcacgg tcttgatccc
     4081 ccggctggcg aaggcctcga tgttcttctt cttcaggtca tcgatgaacg ccgtctgccc
     4141 cgtccggatc agcggcgagc cgcagcagac ctggtcgtgg gggacgatca cccggatgcc
     4201 gttgcgcttc atgacctcca tcgcgtcgag cgccgtctgc ggcacccggc cgttgaacat
     4261 gcacccgacg aagaacccca cctcgccgcg gacgggaccg tcgggctcga tcacctcggg
     4321 caccatctca aggaacgtgg cttttgtccg ctcgacgctc cggcccgtct cctgcaccat
     4381 cttcgccacc tcctggtggc gggggagggt cagcccgcga cggtttgcca gttcccggag
     4441 cttctcaatg gctttgcccg ggacatcgat ctccttcggg cagacctcca ggcaccgctt
     4501 gcaggtcgtg cagtagaaga gccccttctc gatggcatcg atgatccggt cccccgagtc
     4561 gcgggggtcg agggcgaggc gcatctcctg ccggagcacc gtcgggccgg cgaagtccgt
     4621 cacctggagc gccgggcagg ccgagacgca ggagaggcac tcgatgcagt cccggagcgg
     4681 cttgatagcc tcgatctcct ccagggtcgg gagtcctgca tccggcgccg ggcagatccg
     4741 ggcgatcttt gcgatgaccg ggctcatatc caccgtcagg tccttcaaga ccgggaggtc
     4801 cagcggctcg atcgtcatac cgtcgcgggc ctcctccatg caggcaaggc cgggcgtccc
     4861 gtccacccgg acggcgcagc tcccgcactg ccctgacccg cagcagtagc ggtaggcgag
     4921 cgtcgggtcg tgctcgtcgt ggaccgcgtg gagagcgtgg agcacccggg cgccctcgtt
     4981 gacccggacc gtatactcct cgaagtggga ctcgtcgtcg acggcggggt cgtagcgggc
     5041 gacccggagg gtgatctctt tcatgccggc acctcccgcc gctcgatccc ttcccgggag
     5101 atcgagacgt aggtgtggca gaacggcgac gtcgccggat ctgtcttctc cgcatcccgc
     5161 cggacgtggg ccccccggtt ctctggccgg agcagggcgc acctcacgat cagggacgct
     5221 gtggtgcaca tgttccggac cgtgcagcac tcgagaaggt ttgcggtcga ggcggcgcag
     5281 agccgctggt cggcgagccc gcgaatatgc ccgagcgccg tccggagatc gggggcgttc
     5341 cggaaaattc ctgcctggtt ccacatcgtg agtttcaggt ccttccggac cgttgcgggg
     5401 ctcacggtcc cctccaggaa agcatcgagc atatggatct tctcgtcaat ccgggcggca
     5461 tcgacctgac cgttcctctc cggcgacttc ccggcgaact ccccggcacg tttcccgaag
     5521 acctgcgtct ccgcgagtgc attgccgccg aggcggtttg cgccgtgcac cccgccggaa
     5581 acctccccac aggcaaagag gccggggatg gtcgtccggc actcaggggt gatccggaga
     5641 ccgcccatga tatgatgggc ggtgggcgcc acctccatcg gttccctcct gatgtccaca
     5701 ccgtaggcga ggaactgctc gagcatcacc gggagccggg tctcgatcct ttctcgcggg
     5761 aggtgggtca cgtccaggta gaccccgcca cggttcgttc cccggccctc cagcacctcg
     5821 gtcgcgatcg cccgggccac cacgtcccgg gtggagagtt ccatccgttc gggatcgtag
     5881 cgctccatga accgctcccg gcgcgtgttt aagaggattc ccccttcgcc ccgaactgcc
     5941 tccgtcacca ggcggccgcg agcgtcgtag ggatagaccg cacccgtggg gtggaactgg
     6001 atcatctcca tatcgatcag ttccgccccc gcccggtagg ccatggcaaa tccgtcaccg
     6061 gtcccggcgg cggagttcgt ggatatgtcg tatgcctgtg ttcccccgcc ggtcgcaagc
     6121 accgtggcgt cggaccggaa gagcatgagg ctcccatccc ggtccagtgc gatggcgccg
     6181 gcgaccgcac cgttctcatc ttttatgagg tcgatgaccg agacctcctg gtaaagccgc
     6241 gtatcggtcg cgtcgaggcg gtcgaggagg gtcatgatca tctcgtggcc ggtccggtcc
     6301 ccggcgtagc atgtccgggg gaaccgctgc ccgccaaacg gccgctgggc cacctgccgg
     6361 tcatccgtga cgtcgaagac cgcaccccac ctgatgagat ccgcgatccg ctccggtgcc
     6421 tcatgggtca gtgcgtccac gagcgcgggg tcgtttaagt atgccccacc tttcagcgta
     6481 tcctcgcggt gcacctgaac ggagtcctgg tcccgcaaca cggcgttgaa gccgccttcc
     6541 gccatcgtcg tgcacccgcc tttgccggcg atggtctttg agaccaggac cacgccgccg
     6601 taccgggagg cctcaatggc agcccgcact ccggcgccgc cgcttccgat aaccagcacg
     6661 tgcgcgtcca caatctcgtc tacaagcatc ttattaatcg gtgcgttgta taataccata
     6721 aagtaattgc aggggacaag tgagccagaa tgaggctttt aatgaaattt ggcggcacct
     6781 ccgtcggaga gacagactgt atccggcggg tcgcagacat tgtagaatca cacaggcaag
     6841 caggcgatga ggtcgcggtg gtcgtatcgg catgctcggg gatcaccgat cagatcgtcg
     6901 cggtggctga cgaggtcata aagcgcaagg agcagccccc gatcgggacg cttctttcgg
     6961 cgatgcggac ccggcacatc cggctccttg gcgaggtggc tcccgactac atcgacgagg
     7021 tgacggcggt catcgacgat cggctcaccc ggctgcagaa catcctcacc gcagtgcata
     7081 ccttgaagga gctgactccc cggtcccgcg actacatcat ctcctttggg gagcggttgt
     7141 ccgccccgat cgtgagcgcc gccctccggc agtgcggcat ctcctcggtc gtccttgacg
     7201 gcgccgaggc cgggatcgtc acgaccgcaa accacggaga cgcccgtgcc ctcccggcga
     7261 gtgagacgag catccggagc cgggtcgcgc cgctgctcgt cgatgccgtc ccggtgatca
     7321 tggggttcat gggagcgacg gagcagggtg tcatcaccac gctcggacgg agcggttcag
     7381 actactcggc cgccgtcgtc ggagccggga tcgatgccga tgagatctgg atctggaccg
     7441 acgtcgacgg ggtgatgacc tccgaccccc ggatcgtcaa ggatgcccgg gtgctcgacg
     7501 acatctccta cctcgaggtg atggaactct ccttcttcgg cgccaaggtg ctccacccgc
     7561 ggtcggtcga acccgcgatg cagaaggata ttccgatccg ggtcaagaac tcttttaagc
     7621 ccgatgttcc cggcaccctc atcctccgcg atgagcacca ggagaagcgg gtggtcaagg
     7681 ccatcgccct gatcgagaag gtggccctgg tcaacatcaa cggtgcgcag atgatcggcc
     7741 gccccggcgt cgcaaaaacg atcttctccg cgctcgcgga gcgggaggtg aacgtcatga
     7801 tgatctcgca gggttcaagt gaagcaaaca tctccctcat tatcgatgag tctcacctgg
     7861 aggccgcgat ctgcgccctc accccgatcg tgaagcaggg catcgtgcgt gaggcaacct
     7921 acgaccggga tgtctcggcg gtcgccgtgg tgggtgcggg gatggccggc accccgggga
     7981 caggcggccg gatcttctcc gcgctcggcc gggccgggat caatacgatg atgatctcgc
     8041 agggctcaag cgaggtgaac gtctcgttcg tcgtgaaggg cggagacggc aagcgggccc
     8101 tgcaggtgct gcacgacgaa ttccgactct cggagaactc tgacgacacg gaagatgcct
     8161 tcgcatcttc ctgtcaggca cagccctccg ggttgtgccg tgccgaacat acctaccgtg
     8221 aagcaggggt cgatatcgat ctcgaggccc gggcggtccg ggcgctgatc gactcgctca
     8281 cctaccgaag aaccggtgcc ttcccgatgc tcgggaaggt cgggcacttt gccgggctga
     8341 tcgactgcgg cccctatgtc ctcgcgctcg cggtcgacgg cgtcggcacc aagatgctcg
     8401 tcgccgacgc cctccaagac tggcataccg tcgggatcga ctgcatcgcg atgaacgtca
     8461 acgatctcta cgtgatgaac ctcgaaccgg tggcgttcgt cgactacatt gcgaccgacg
     8521 cgctctcgcc cgagaagatg gcccggatcg gcgagggctt aaacgaaggg gcaaggcttg
     8581 cgaatatgaa catcgtcggc ggcgagaccg cgaccctcaa ggggctcgtg aacggcctcg
     8641 acctcgcggg gacatgcctc ggggtccaga agaaggagaa ggtcgtcacc ggcgagggga
     8701 tcgtccccgg cgataagatc gtcggcgtcc cctcgagcgg cgtccacagc aacggactga
     8761 cgctcgcccg caaggtcgtc gaggattgcg cctcctacga cacccgtctc tcgaacggca
     8821 aaaccctcgg cgaagagctc ctgacgccga cccggatcta ccacgaggtg ctccgggtga
     8881 cggaggcatg caccgtccac gggatgtgcc acatcaccgg cggaggcctc ttaaacttcc
     8941 agaggctcac ggagtacggg ttctccgtca ccgatcccct cgcggtctcc cccatcttcc
     9001 gctggctgca ggagaccggc ggcatcagcg atacggagat gtaccgcacg ttcaacatgg
     9061 gcatgggcta cgccttcgtc gccccggcgg agagcgtggc ggcgatccaa tcggttatcc
     9121 ccgacgcccg ggtggtcggc gtggtgacgg aagagccggg ggtgcggttg aagggtgtgg
     9181 agatccagta ggattttcct tcttttttga gttactggtg cccgtgcggc gttactcgcg
     9241 agtcacgggg ctcatcctct tcaggtgtga catccccgag tgtcacacca gaaaggagta
     9301 taatcgcgct aaagacgatc aaggccagtg aaacagttag ggaaagcccc atacagtgga
     9361 ccggggggat atcgcccttg gggggagcat cccccctccc ctgtttccaa ctcacacctt
     9421 ccggtgctca aactcctgct cgaagcctga tggcttctca agctccgggc gttccgccca
     9481 tcgcaccctt cggccagtcg cttatcccta tttgaggcac tcagcgagcc cccaccccgc
     9541 ccggcctccg gcctcctcaa acgcccctct ggggcggggg cagtcgtggc gatatgcgat
     9601 ggacagaacg tccggagcag aagcgccgct aggtgcgcag ctcgatggaa agccgagcac
     9661 gggttcgtgg tgtcttgaag aacgacggcc ggaacaccca gagctcaagc atccggcaaa
     9721 tgcgagtgcg agcgaagaac tctgtcctcc tcaaactcac gtcccgccgc cttcctcctc
     9781 gaagtagtgc agcgcgtcgg gaacctgctc ccggatccgc cgcaggaagt ggtttgtgag
     9841 gtcggggccg gctaccagga gcgagtgctg catcgcccgc tccgtatcct tgctgagctc
     9901 cccccgctcg tccatctccg ctttgacgtc gtggacagcg ttctcgactt cctgttcgct
     9961 ctcccacttc agcaccgata tcgttcgtgc catctctctt cacctcaggg cgtgccgtat
    10021 gagaggaagt cctattttag gttaaccccg gcgccggggt aaaaaagaaa agtatcaggt
    10081 ttcgccctga tcgaggagca tcttttcggc tttttcgctg acgagcatgc tgttcccgga
    10141 ggggtcctcg atgatgaccg tgaatgggac ggccccctcc cgtgcagccc cgatcatctc
    10201 ctgcatccgg agtgcggctg cctgctcgtc gtcgctctcg gggttcgcga ggatcgtcgc
    10261 caccgcctgc tcgaagcggg nnatctcgat cgtccccatc gtactccgga ccacccggat
    10321 cgcgagatcg tcggccccct cgacccgcac cgtccacctc accgggtcgc cctccccgag
    10381 aatgatggtg tcggtgtgcc ggtagccgca ggcgtcgcag gcgatagcga cgagcaggag
    10441 atcggggaag tgggggatct caagacggtg atggacgatc tggatctcgc cgctgcacgc
    10501 ggggcaggtc ccggaatagc actcccgcac tagaggcccc cgccgatctt ctcgcgtgag
    10561 accttgacgg acataggcgt gatgaggacg tacttctgct ccccgaggcc gacgatgtcg
    10621 ccgttcacgt cctttgccac gtcgcggagg tcctttagga cccgctcgaa cgtcaccttg
    10681 tccatcttca gccgcccgat gtcgacgatg acgatattgc cgttgtagac ctcgtctttg
    10741 acgcggggag tatccttgag gtcggcgatg gtggcgatct tgatgtacat ggttgccggt
    10801 tcttcttcgc tcgacacctc ataggaggcg agatcgagtt ccatgtagtc ctcttcgttt
    10861 cgtgctggac ttttaccgag gatgctgtca aagagctttt taaccataga aaaagtgagg
    10921 aatgaatttt atttaatagt atcttttaat ttctccgtca gatctcgaga ttccagatat
    10981 cgtctccgac gtagtgaacg cttttgaccg acttgccctt ctcctgccgc tccatatcgg
    11041 cggcatcaag gagcgcaatc ccgattgcaa gcggtttgcc gtagcgctcc tcgacgatct
    11101 ggacggggtg cccctcccgg atgtccggcg agattgagac gatccccggg cgcatagcat
    11161 ccgcgccgtt tgcaacgaac ctgaccgcgc cggcatcgac caccacccgc cgctcgggga
    11221 tcgggtgctc aatgaggccc cgcaggctgg gaaaggccca ggtctcagag gctatcagga
    11281 gcggttttct gtcgacgagg tagatcccga cgggggcatc cgtctccacc cgctcgatcc
    11341 ggtcggaccg gaaatgatcg gccgacgctc cgatctcgtc ggccagctgg cccaggagat
    11401 cggctatctg tgacttccgg atgacgtgac gtttttttac ggtgatcttc ggcatgtaat
    11461 cgagacctcg aggaagtgtt aaccctcgcg caacaacgcg gttatattta agtagcttcc
    11521 tcgctcaaat atattactcc agaaaggacg gcatagggta atcatatgac gccaagaccg
    11581 ttggatattt tagatcaggt actgaatcgt cagcccgtta tcatcagcct caaaggtggg
    11641 agagagatcc gggggatcct ccaggggtat gacgttcaca tgaatctggt attggataag
    11701 gcagaagaag aagcggacgg cacggcgcag aaactcggca cgttgattgt ccgcggtgac
    11761 aatgtgatct acattacccc ttcagttgaa taaagtcagg tgagagagaa tgtcaaaagg
    11821 cacaccctca atgggcaagc ggcagaagcg cacccacatc gcctgcaggc gatgcggcaa
    11881 gatctcgttc cacgcacagc acaaggtctg tgcgtcctgt ggcttcggca gaagccggag
    11941 gctccgtagt taccgctgga cggaaaagaa ggcaaaggta ccgacgcatt agagttgtat
    12001 catgtgtggt atcgttggca tcgtcgatgc tggcggtgtc tcttttccgc tgtactacgc
    12061 cctgtacgct ctccagcacc gggggcagga gagcgcgggg atctctactt ttgaaggcgc
    12121 gaccctgtac acgcacaaag cccagggact cgttgctgag gtcttcaaca gccagaccct
    12181 gcaggaactg cagggcaacg ctggaattgg acacgtccgc tacccgacaa ccgggtcgaa
    12241 ggtcccggag aacgttcaac cgttcaactt caggtaccgg gggctcgacc tctcgatcgc
    12301 ccataacggc aacctggtca acacggatga acttcgggag gattatgagc gccgggggca
    12361 gatcttctgc accacgaccg ataccgagat catcgggaat atcattgccg atgcgctccg
    12421 gacctcaagg agcatggaag atgccgtccg gctctgcatg cggcgcctgc ggggctccta
    12481 tgccgttgtt gcgctcttaa acgacaccat ctacgccttc cgcgacccgc ttggtatcaa
    12541 accactttgt atcggcaggc tcggccgggg ttacatcgtt gcatcggaga gcgtggcgat
    12601 cgatgcactg gacgggacgt tcatccggga cgtgctgccg ggagaactca tccgtatcga
    12661 tgcaaacggg ctctccttca tccagatagc aaccgccaac cggagggcgc attgcatctt
    12721 cgagtacatt tacttcgccc gcgccgactc ggtcatggac ggaacgctgg tctatgacgt
    12781 gcggcggcgg atcgggcaga aactctatga tgcgagccct gttgaggggg atacggtctg
    12841 cccggtcccc gactccggca ttgcgtacgc cgccgggtat gccgaacgat ccgggatccc
    12901 gttcgtcgag gggctgatga agaaccgcta catgggccgg acgttcatca tgccgaccca
    12961 ggagcagcgg gagcgggcag tcaggatcaa actcaacact gtcaggggaa acctgaagga
    13021 caagagggtg gtcctcgtcg atgacagcgt cgtccggggg acgacgtccc gccggatcgt
    13081 gaacatgatc cgggaggccg gggcggaaga gatccacctc cgggtcggtt ctccgccgat
    13141 cattgccccc tgctacctcg gggtggatat gcccacccgc acggaactga tcgcgagcgg
    13201 caaagaggtc gaggcggtgc gggagagcat cggcgcgacg tcgctcacct atatcccgct
    13261 caaggacctg gtggaggcga tcgggtgcgg cgagcggaac ctctgcaccg ggtgtctgac
    13321 cgggtgctac ccggtgggta tccacgggga gaagagttgc cactgtgtcg tggactacgt
    13381 ggccggcacc caccagtctg acctctcgac cttcagggcc gggaaggaac agacataggc
    13441 ggtttctccc ctgcgcgctg tcgtatcccc catcccttat tttgggaaac ctgagctctt
    13501 tttactcgag agaccgagca cccggaaaaa accccggttc tcgcgaggaa ccgtttcgcc
    13561 ccacgcacct gcaccgaccg gttcaaccgt caacggggat catgcggggt tggggctgtc
    13621 tgtgagctca tcgagcgcga gcgtgcaggc tcgaagggag aaactgttgt aaagcagtaa
    13681 ataggataca aaaaatgcgc aaagacgctg aagaacttaa catagcgagg gatggatttg
    13741 aaccatcggt ctacgggtta tgagcccgcc gggatttcct gactacccca cctcgctcct
    13801 aatctgtgag gtatataaca ttgtttcgcc gaggatatat acctttccgc ggtcagaatt
    13861 gccgcaactc gaagtcctcc ggtcttctca aactcctgcc gtgccggcag tcgttcttca
    13921 aaaccctccg gggctcaagc ttcgctccgg taggagcgtc gcacctcaca atcttttata
    13981 ggggcatgta gcatacctgc gttgtatgga agagaacaga ccggtggacg acgacctgca
    14041 gcgtctccgg gaagaacgtc tccgggaact cgaggcccgg atccagggcg gtacgggcgg
    14101 cgtcatcgag gtcgtcgacg acaggtttca ggagatcgta cggaagcact ccttcctggt
    14161 cgtcgacgtc tgggcggaat ggtgcggccc ctgccggatg gtggccccgg cgatcgagga
    14221 cctcgcgcgt gacttcgccg gcagggtgac cttcggcaag tgcaacgtcg atcagaaccc
    14281 cgccctggcg tcaggtttca gcataacggc gataccgaca ctcctcttct tcgcgaacgg
    14341 catgctggtc gaccgggtgg tcggcgccct cccgaaagaa gccattaaag caagggttat
    14401 gcgggcgttc gggaccggct agaggctccc gccctgccgg cacctgagcc actcggagag
    14461 ccgcgcggcc atgataaagt cgttccggga gagcccgccg atggcgtagg tgtaccaggc
    14521 gacatccacg aaccttcctt cgtggatgcc gatatccggg aggtggttct cccgcgcgga
    14581 gaagatgacg atctcccgga ggaatatcac ggcttcgttg aaccctttac aggcgaaccg
    14641 ggccgagata tggccgtcct cgagcgacca ctccgggatc tccggtgcaa ggtcggcgat
    14701 ctcccgccgg gtcagcggtg ccgtatcccc gacatccggg ctgatcggtt cggacgaaag
    14761 atccatggtg atccctcttc cgtgcagggt gaaaaaggtt cttcttcgga gggccggtga
    14821 gacgccgctg ttttcccgga aatccgttta atctggttag attgggggcc gggtttcagc
    14881 gcagcttctt tatcaggaac gcgacgttct cggcgaaccg ccggactgtc ttcatccctt
    14941 cctcgtcgtt taaggcttcc cccggtgctc gcccaaagac catgttccag tacgtggagc
    15001 ctggtacgat catgtcgttg atgaggaaga acatcagcat ctcctggagc gtggcggtat
    15061 gcccaccccg ccgggccacg gcgatcgggc cgcccacctt ccgcgagagg aacgagtccg
    15121 acgacatcga gaccatcccg attcgctgca gtgctgacat aacgtcgccc cgtgcggtcc
    15181 cgaagtagac cggggtgccg acgatgaacc cgtccgcctc ccggagttta tcgatgatct
    15241 cgttcaagcc gtcatcgagg gcgcactccc ccccaccgca gagtccgcag gcggtgcacg
    15301 agaggatgtg catctccgca agcagcacgg tctcggtctc gatcccctcg tggtcgagga
    15361 cctttgcgca ctccccgagc acctgcgcgg tgtttccctc gagccgcgga ctcccgcaga
    15421 gcagcagtac ttttccgttc gtcatctcac acctcaatca ggcggtaggc ctcttccccg
    15481 aggccgatcc gggcagcgta ggcgatctga tagctgccgt ccgtatagtc ccagaccccc
    15541 ttaaatttgt cttcccccgg ggcgaggttc tccgcaaggt gtgtccgggc gaacccctgc
    15601 ttgctgttga cgaggtcgag gctcgcgtgg tcgatcgcaa ccggatcggt ggaggcgagg
    15661 atcccgatat cggggacgat cgccgcgtcg ctccagggaa cacagtcgca gtccggggtg
    15721 atgttgagga ggaagttgat gtagcccact ctcccgggtt taccacggac agctccgagg
    15781 gcgtactcgc agagcctctc cacgaacggc ccgatctccg tcgtccagtc gaactcaatc
    15841 gcgcctgtct cgcaggaacg catgcagtcg ccgcacccga cgcagtgctc cgggttgaac
    15901 tgcgcccgcc cctcgacgat ggtcattgcc gcctgcgggc agacctggac gcatctgccg
    15961 cacccgacgc agttctcacc ctccacgagc ggccggcccg cgtgctgctc cgccttcccc
    16021 gagggcgggg cgcagcccat cgccaggttc ttgatcgccc ccccaaaccc ggcgagatcg
    16081 tggcccttga cgtgcgagag gacgatcatg ctgtcggcat cgaggatgct ccccgcgatc
    16141 ctggcgctct ggaagtgctt cccatcgatg gcgacctccg cccagtagcc gcctgcaagg
    16201 ccgtcggcaa cgatcaccgg ggcaccgacg actgcgtaat cgaacccgtg ctcgatggcg
    16261 gtgacggtat gcctgacggc gtcggccctg ctccccgcgt agagggtggc ggtatcggtt
    16321 atgaacgggt gcgcctggcg ctccttcacc ttgtcgacca cctgccggac aaagagcgga
    16381 tgcaggtagg tgtcgcaccc ccgctccccg acgtgcagct tgacggctgc aaggtccccc
    16441 ggccggatca cctcaccaaa cccggctgca tcgaagaggc ggcggatctt ctgtactttg
    16501 ctctcccggt ggctccttgc cctgagattc gcgaaataga cgtttgccat cgttgtctgc
    16561 tccgtcttgt aagtagtctc tgatcgtcgg aggataagaa ttgtgcctgc actcccgacg
    16621 ctacggtggc ggataacggc cgggtctaca gcccggctct ccggcgcatc ggccggacct
    16681 cggcgccttc catgttcccc gccgcccagg tgtagcgttc gcgatctcgt tcgatgcgag
    16741 agagagaacc gagacccctt cgtagagttc ctcgctgatc agtttcataa gcacgtcagg
    16801 caaggagacc cccggtttta accgggggag gaattgcccg catcaggccc cgtacacgtt
    16861 cgccccgcgg gag
//
LOCUS       DAJB01000063            3995 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_117183, whole genome shotgun sequence.
ACCESSION   DAJB01000063 DAJB01000000
VERSION     DAJB01000063.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_117183"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          708 a         1282 c         1321 g          684 t
ORIGIN      
        1 ccttcacccc cgcaagttaa aatctcggat caacaagagt cccgacaacc gcctcccccc
       61 ggagtgcctg gcggacgcgg tcgtcgtgcc tgccgttaac cacccgggcc cgcagattgt
      121 gctcgaagac gaaccggatg aacgcaggat cgacctcgtc gggggtgaga gaggggtcct
      181 caacccgtga gaggagcttc ctgtggtggt ggatgccgtc gaccgatttg aggagaagga
      241 ggtcgagcga gagttctttc gcgacccagg cggcgatggt gtcggaggtg acgttccagg
      301 agtgagggag ggggtctgcc cgccgcaggg cgcagtaggg gaggaggacc gtcacctccg
      361 ccggaagcgt gagagcggag accgccggga cgtcgtgcga ggcgatgtac cacccgaact
      421 gctccatgcc ggcgatcgcc atccagtgac cggcggtatc ggagacgttg aggcgccgga
      481 cgaggtcggc gaacttcccg ccgccgggca cgatcagcac cgggcgcccg acctcccgga
      541 agatctcgag caggggcacg acccggtcaa agaggcttcc gcccaccttg acgaccagcg
      601 ggggtgcaag cgttgtcata tcgctctcct attaaaccgg gggatacata accctgcgta
      661 tgcgccggat cgccgcgctc gtcctgctcc tctgcctcct taccggcaca gccggcggtg
      721 tgcggatcgt cgaattctgc ccggacccct accaggccgg cgaccccgac gagtacctgg
      781 tgatcgaggg atcggggatg ctcgacgggt tcgtcctctc ggacggcgag ggggggtacc
      841 ggttcccgcc gggaaccacg atcgacgggc ggctggtgat cgcgaggagc ggccccgcgt
      901 tcgagcaggc ccacggttac cctcccgact tcgagatcga ggagcggaca tcggccgtgc
      961 cggacgtgat cccgaacggg aacctcctga tggccaacca ggccgacgaa ctcctcctct
     1021 accaggggac caccctcgtc cagcaggtcg cctggcccgc cgacgtcctt ccccgggaag
     1081 ggcggatcca ctacctcgat ggcgagatct gggacccgcg cccgctctcc atcggccagt
     1141 cccgccttct acccgcaacc tttgagggcg tcgccgtgaa ggccttcgtc gccccggact
     1201 gctcgtatga ggtcttctcg gcagccgtcg cgtctgcaga gcacgagatc ctcgtaaacg
     1261 tctacgagtt caccgacccg gctatggcag ccgatctcat ctgcgcccgc gagcggggcg
     1321 tgacggtgac ggtcctcctc gagggcggac cggtgggcgg tgtatccgcc gaagagcggg
     1381 cgatcgcggg tgccctcaac cggagcggca taccggtcct ctcgatgacg acgaccgatg
     1441 ccgcccacgc gaagtaccgc tacgaccacg ccaagtatct cgtcatcgat ggggagaggg
     1501 tccttgtcgg gagcgagaac ttcaagcccg gcggctaccc ggcgccgggg ctgcagggca
     1561 accgcgggtg gggggtctct ctcgagaacg ccgggctcgc ggcctacttc cgcgaggttt
     1621 tcctcctcga cgcggcgggc ggagatatcg tccccttcga gggcacgacg gccgagcctc
     1681 gcacaccctg ggctccctca tacacggtgg agtttgccgc ctgccgcgcg gagggagcgc
     1741 gggtgacccc ggtcgtctct cccgatacaa gcgctctcat cctcgggctg atcgaggggg
     1801 cgcaggagag tatcgcgatc gagcaggcct acatcacgaa cgagacagca tacgacctga
     1861 acccctacct tgcggctgca gtcaacgcct cccggcgagg ggttgcagtg cgggtgctcc
     1921 ttgatgccgc ctggttcaac accgaggacg acgcggacaa cgacgagatg gtcagcgtga
     1981 tcaaccggat cgccgcggcc gaagggctcc cgctcgaagc tcggctcgcg gacctggagg
     2041 cgaacaacct ggctaagatc cacaacaaag gggtcatcgt cgacggccgg acggtgctcg
     2101 tcagcagcat caactggaac gccaactcgc cggcgttcaa ccgggaggcg ggtgtgatca
     2161 tcgagcaccc ggatatcgcc gcctactaca ccgcggtctt tgaggacgac tgggatgcct
     2221 cgggagaggg cggggcgagg ggcactaccg gaccggaccg gctcaaaata atcgcagcgg
     2281 cgtgcatact cgccgccctt atggggctct acctctatcg gcggagacgc acctgagacg
     2341 gccgctcacc atctaccggt aagatcggcg gagcggcaga cgtcgcagat agtgatcgtc
     2401 ccttcgcccg aggcgacggc ggagacccac cgttcgatga ccgtctcgag gtcgacgggg
     2461 aagtcctgcc gggtatatcc gcgtttgcgg ctcatatact gcgataccgc ggccctgctg
     2521 atcccgaggc gctttgaggc ctcgctctgg ctgatgccgc gctcgttcac caggcgatag
     2581 accatctcag cacgcatctg gggaagcagc ctccggacga gggtgtcaca gcgcatgata
     2641 tcgcaggaag ggggctcggt cattgcacgg acaccagctg tgagtctttg tactcgtaga
     2701 ggatctgccg ggcgtccctg aaattgaact tctccacgat cagatcgtta tctttgagtc
     2761 tctttaaggc atagcggaca gtgcgggggg ccaggctgct gagacggacg agctccttgt
     2821 gggtcaggga acccccatct tccagcagca ggaggatctt ccgggaggac gggggaaggt
     2881 tcacatcatc catgcaggat agttaacgct gttaacatat caaactgacg gatgttataa
     2941 tcctgaaaga ctataggaga tctatgctgg aacggcagga gaaaacggca ctcgccgtgc
     3001 tcgtatgcgt cgtcgggatc gtgcttgcgg cgcacctcct cttcgacgcc gtcggccggc
     3061 cgctgctggc ggacccgtac tcagaaacgg tcccggacgg agcgctggtc ctcctggaag
     3121 ggagtatcga agggatcaaa gagacctcga ccggcgagca cctgatcctc accgtcaacg
     3181 gcaccccggt ctttctgccc gagaacgtgg ctgctgcgct ggaacttcac gagaacgaga
     3241 acgtaacggt ctatggggtt gtccagacct accgcgggga gcgggaggtg gtggtggcct
     3301 ccgccgcgga tatccgggtg ctgaaatgac ctatttcttc gtgtagaaga tatcggcgcc
     3361 gtcggcatcc ccaaagagag gccgggtgtg tcggggcttt tccgggctct cttccgtctt
     3421 gctggccccc ggttcgggct ccccgcgggc aggcgccggg gtctcgtcgg gtctcttctt
     3481 tcgtcccccc tccacaccgg tcccggaggg aaggtctccg tcgttgatcc gcacgctcgg
     3541 catacaggag gatcgcccgg gccggtatat gacaccttgg atcagccgcc ggcctctcgg
     3601 cggcggaaaa tccggaaaga agttcacgcc gcagcagaga aactcaacca tcgcaaaagc
     3661 agcagtgatc cgggcagaag gggccggcag cgcggtaaac cggagcggcg cggaggacta
     3721 cccgctctcc gcggtccccg gcgatgcccg gcaggggttc tggccgatca ccctggtgct
     3781 gcttgggttc accttcttct ccgccacgat gtggggcggc ggcagcctcg gggttgcgtt
     3841 ccggttctgg cccgacctcg tcgggatcat cctctgcggg agcgcgatcc tcgctgtcta
     3901 tgtcgccggt ctcgggtatg tcggctacaa aagcggcctc tccacagtcc tctccgccgt
     3961 aagcgcctcg gtcgccctcc gggacgccgg aggcg
//
LOCUS       DAJB01000064           14802 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_54592, whole genome shotgun sequence.
ACCESSION   DAJB01000064 DAJB01000000
VERSION     DAJB01000064.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14802)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_54592"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    206..407
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3133 a         4020 c         4396 g         3051 t
ORIGIN      
        1 tagcgattgc gcgggacatt acggatcgga tccgtaccga gagcgagctc aagataaagg
       61 agagcgctct tgaatcgtcg acgaacggca tcctcatcgc cgatctcacc gggaccgtca
      121 tgtatgccaa ccggagtttt gcctcgatgt tcgggtacgg agaggggggt gcaatcacgg
      181 ggagaaacct ggagggattt tttgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat gcggagatga
      421 tcgacgaccc gctcgtcgca aagatcctcg atcagtcgtg ccggattgac gggatcgtca
      481 ccgagctcga ccggggatgg gtcgagtcgg cgaacgtcag gcagttcctg cgaaagcacg
      541 gggacggtgc ggagcctcct ctgcaggcgg ttgtccggat ctcgaccagg gacgaagact
      601 gattctcccg ggagataaag ccgtcatctt ttcgccgagg atggcctgca cgctcttctt
      661 agatgaggtg aaaaggcccc tcgcgggttt gagccacgta aagcgcgtct gctctgagca
      721 gaatactact gacagggatc tcttccccaa aacaagaaac gccccggccg ggattcgaac
      781 ccgggtcgaa agctccggag gctttcagga tatccactac cctaccgaga ctgccatatg
      841 atgttgtcgt gtatggtata aaaggccagc ggagaggagg ggttcaaacc ctccgcgatt
      901 catagagctg ccagattttg cacgctcctt cgtggctgac catgcagggg cccaccgggg
      961 tgcgcggggt gcaggccttc ccgaagagct tgcagtccga cggccgggcg atgccgcgca
     1021 gcactttatc gcagatgcag gcggaatgct tgtccacgtg ccggatctcg atatcgaact
     1081 tcttctgcgc gtcgtagccc tcgaactccg gtttcaggcg gaggccggat gccgggatca
     1141 cggggaatcc gcgccattcg acgtcgaacg gctcgaagac ctcgtacatg aggcgtttgg
     1201 ccttgacgtt cccctcccgg gtgaccacgc gcgggtaggc gttgtccacc cggtgcaccc
     1261 cctcgtggac ctgctgcacc gccatcagga gggcgaggag gatatcttca ggctcgaacc
     1321 ccccgacaac ctggggaacc gggaactgct cgtactcctc gtagcccatc accgcgcaga
     1381 cgtgccccgg gaggatgaac ccgtcaagcg ccgcctcccc ctgaccgagg agccacgcca
     1441 tcgcgggcgg gacgagccgg tggcacgaga ggatggagaa gttttttggc gggcgagaga
     1501 ggatcgtggc ggcgaccgtc ggcgcggtgg tctcaaagcc gaccgatacg aagacgacct
     1561 cccggtccgg ctctcttctc gcgatctcca ccgccttgtg gacgccctgc accacccgga
     1621 tgtcgccgcc gctcgattca agcgacccct tcgacccggg aacacgtaag aggtcgccgt
     1681 aggtggcgat ggtgcagtcc cgctccacca ggtcgagcgc ggcatcgatc tcgccctgcg
     1741 gcgtgatgca gaccgggcag cccggcccca tcacgatctt gagacgctcg gggaggatac
     1801 tccggagtcc ggcgcgcgcg atcgccgcct cgtgcgtccc gcagatatgc atgagcgtga
     1861 tatcccggtc aacgagggca cggagcgtat cgaggatttc cttaccaacc gccatgaatt
     1921 atcatgctta tatctcaccc agagcacata ttagtaccgg aatgaacagc atcatctacg
     1981 ggagtgcgct cattgtcacc gggatcatcg ccgcgttcgt cgtccatgaa gtgattcggt
     2041 ggctgcagaa gcgggccgac ctcaccgaat cgaaactgga cgacatagtc ctctactcgc
     2101 tcgacaaacc tctctgtatc tttatagtcc tgggttccgc cgggatcgcc gtaacgctct
     2161 tcgtcgaccc cgcggccgtc ctcggggagt acgcgtggct cctctccgag aagtacttca
     2221 ccgcggtggc aatcctcatc gcggcatggg cggtctcctc gttcgtctac agtttcatca
     2281 acctctacgg caggtggatg gcgtcgaaga ccgagaccga cgttgacgac cggatcatcc
     2341 gggtgctcga ggtctcggca cgctacgtgg tctggttcat cgccatcctc atggtcctcc
     2401 ggatgctcga ggtggacatc acgccgctca tcgccggtgc cgggatcgcc ggcctggcgg
     2461 tggcgctcgc ggcccaggac atcatctcca acttctttgg gggtgcggtg atcctcatgg
     2521 acaagccgtt cgcggtgaac gaccggatca agatcgacaa ctacctcggg gacgtgctca
     2581 gcatcggccc ccggagcacc cggatcaaga cgatggacta ccagatgatc accatcccga
     2641 actcaaaggt cgcgagcagc gtcatcacca actacgccat gccggacgtc aagttaaaga
     2701 tcaaggtccc gatctcggtc gcgtacggca gcgatgtcaa gcgggtaaaa gagatcctcc
     2761 tcgagatcgc gggcgaggcg gcggcgcggt cggaatgcgt cctctgcgac cctgcaccga
     2821 ccgtctactt ccttgagttc gccgattcga gcctgaactt catgctcgtc atctgggcga
     2881 aggccttcaa cctggcctgg gacgtccagg actacgtgaa tacacgcgtc tgcgagcggt
     2941 ttgcagaaga gggaatcgag atcccgttcc cgcagatgga catccacaat cggcactgat
     3001 caggctgcac gataaaatac tcccctattt tcccgtacag gctcgacccg gccccgggcc
     3061 tcgaggagtc tcaagcactt tgcgacctcg gccggacgga ggccggtaag ggcggcgatg
     3121 tcgtctggcg tgcagggccg gcgccggatg gtcgcaagga tgatctcgac ggcatcctca
     3181 acctccggcg gcaacgcccg gccctccggt gctgccccga tcgcccccgc gttcccaccg
     3241 agcatcgacg cgatccgctc gatagccccg ggcgttgccg gctggaccca gatctcggtg
     3301 ccgggccgat cgagggtgtt cacctggacg cggtccgggc cgatggcggc gatggcgtct
     3361 ttcaggcgga ggagttcctc ttcggtgtcg ttctcgcccg ggacgatgaa gacctcgagc
     3421 cagatctcgc cggggaactc ccgggcaaag gtcacaactc ctgcgagcac ctgcccggcg
     3481 gtgagcccgg gacaggggcg gttgatacgc tcgaagacct cttcggagac cgcgtcgagc
     3541 gacggcacga cgagatccgc ttgcatgagg gcggcccgga cctccgggtc cgtaaggaac
     3601 gcgctgttgg tcagcaccgc gacccggtag cgggggtagc ggtcttttaa gaaggagatg
     3661 atctcgccga tcccggtatg aagcgtcggt tccccggagc cagcgaacgt gacgtagtcg
     3721 aggtccggcg ccgtcgcgag gtaatcgtcg agttccgcga tgacccggtc tgtcgggacg
     3781 tattcgcgcc gctcgcaggt gaggttggtc gtttgcccgc actcgcagta gacgcagttg
     3841 agagcgcagg tcttgtgcgg caccaggtcg atcccgagcg agatgccaag gcgtctggag
     3901 gggaccgggc cgaagaggta acggtatgcc atggggttat cctgagtact ctccggcatg
     3961 aggagtcatc agggttcacc tgtctgcaat gacacccccc ggaggctcag gcccgctccg
     4021 tctcccctat cttgagcatc ttcttgcaga agtggtcgac cgaggtgaag accgctgtgt
     4081 tggcgccgtc gtgggcgccg gcggcggtga cgaacccgag catgtcggag tagtagttca
     4141 gcgaggggta ttttctgcgg aactcgtcga gacagaggcc cgcctgcacc cgcttccccc
     4201 ggagaaaggc gtaggtgtag tcgatgagac agaggacgtg ccccggcaac ttcttctctt
     4261 cggcaagttt ccggaactcg ttgaactctc cttcgcgggg gaggtagagg gcggtccgga
     4321 ggccgtagta gaccggctcg gggtcgtcgg ggaacctttg gagcatgtcg cggatgaggc
     4381 ccagggcatc gccgaggtcg ccctgctcga acttgaggtg gtaggcctcc cggtagaaga
     4441 gcatgtcgtc ggtgaaccgg atgaggccga tctgcagcat cttcaccagc gccacgtcgt
     4501 tgtgctcctg ttttgcccgt gcaaggccga tctccaggaa ccagcggtta tcggggatcc
     4561 actcggtcgc aaggaagatc atcacccaga acggttcggc aagccatggg tcctgctcga
     4621 gttcccccct gaccagccgc tgcagccccg gccgcagctg cccgagttcc tccatgtact
     4681 ggcgtgcgag tttgaggtag ttccaccgtt cggcggccga atacccgggc tcggccgacc
     4741 cttctttgta gaggacgtag tagcagtagg cgtaccagca ccccacagtg gtatcgtgct
     4801 tccggtaggg ctggaggtag gccgcggctg caagcggcat cccgagcttc atcagcttga
     4861 cggcggctgc aaggtggacg ccggctttcc gctctttctt ccgcttcatc ccgcaggcaa
     4921 tttcgcaggc gcggttgaga aacttgatct ctcctgcccg cttgctgacg gtgaagaccc
     4981 ggaacacgat cgcatcgatg aactcgtcat gtgctgcatc ggggatgggg acgcctgcac
     5041 ggtcgaggat cgtgacgacg tttccgaaga acgaggggaa gttctcatcg atggcttcga
     5101 cgttggactc gatgaaggag aagatctcgg ccttggatcg gtcaagatgg tttgcgatga
     5161 caacctccgg gatgaagcca tcagccatat tagctatatc ggagggaatt accataaacc
     5221 atccggattt gtcgccggag gattgccccg tgcaggagtg gagccctgcg gccggctccg
     5281 gtgaacgcat gcaccgggat gcttctgtgg cacggcgggg atggaccggg ccacggtcgc
     5341 gtcgtgggtt gccggaaggt gcatattccg aatctattta tcgtcccggc cggaacattc
     5401 tgtatgtttg gcgtctccac ttactgcctt caccgcgagc cgctctctcg cgcgctcgag
     5461 aatctcgctc ccataaccga atgcgtggag gtgatggatg acgggctgca ctacctggag
     5521 agcgcagagc cgcttgagag ttactcgtat ctctacttca tccacgcgcc gtcacggggg
     5581 gtcaacatcg caagcatcct cgagcccatc cggcgggcga gcgtcgaggt cctgacgcag
     5641 acgttcgccg tcgccgccga ggtcggtgcg gacgtggtca tccacccggg ctactatgcg
     5701 tggagcgagg agcgggagcg ggcggtgggg aagttccggc agtcgctcgg tgaactgacg
     5761 gcggcggccg acgatctctc ggtcacgttc tttgtcgaga acatggggaa ctgggagtac
     5821 ttcttcctgc gctcctgcga ggacctgccg ctcatcgacg ggctcgggct tgcgctcgac
     5881 gtcggccacg cgaacctgaa cgggtgcctg gactgtttcc tcacccaccc ggcggcgcac
     5941 taccacctgc atgacaacag cggcggtgag gacacgcatt ccccggtcgg ggccgggacc
     6001 atcgactttt cagccgtgat ggaggccgtc gcgagggaca acgtcatccc gatcgtcgag
     6061 gtggatacgc ttgagggcgt agtcgcgagc atcggcgccc tggagacgat cgatgctgcc
     6121 tcgcggggca gatagggccg gtcgcacccg gttcctcccc cgggagcacc gtaacctttt
     6181 atagggaaaa actccaacat aaatggtgcg tcacgcctca gtggctcagc cggtagagcg
     6241 cgtccttggt aaggacgagg tcgcgagttc gaatctcgcc tgaggctttc tctgttctgg
     6301 ttctaccctt tgggtaaatg tttatatccc tgttctgtgg ttccttgaac gtgagggcga
     6361 tccccgagtg cgctccggct gcatgcgggt cctggcgttg gtaagatcgt tatacggaac
     6421 gcccggctca gaaagcgctt ttggggagtc gggccgatca ggctggcgtt ttgacgggat
     6481 ctacacggaa aaatgacgac cgggggtaat cttctgacgt tgaaatagtc acgcttatta
     6541 tctccacttt agagtgttag atcaatggtt gattcaatac attacgctca ttctacaaac
     6601 gatcccggga agagtgactg gcagggtctc aaagaacact tacagaatgt ggctggcatc
     6661 gcgtcggaat ttgcgcgaga cttcagtgcc ggtgatcttg cctatgccgg gggcctgctg
     6721 cacgacatcg ggaagtactc gcccgagttc cagcggcgcc tggaaggtgc cagcatccgg
     6781 gtcgaccact cgaccgccgg tgcacgggaa gcaaggacac tgtatccgcg gcagttgagc
     6841 cgcattctgg aatacatcat taccgggcat catggcggtc tcttaaatta cgggagcagt
     6901 gaaagcgggc ttgaggaacg cctgggaaac aggtttctcc cggattactc ggcataccgg
     6961 gatgaaatac cgatccccga tctcacgggt ttccgcccga acttaaggcc ggttcagaag
     7021 aagatagggt tttcgatagc cttcttcacg cgcatgctct actcgtgtct cgtcgatgcc
     7081 gactctctcg atactgaagc gtttaaaaat cctgattcct ccgccatcag gggacagtat
     7141 ggatcgcttg atctcctctc ccggaaattc gatgactata tgaccgcatt cctctccgat
     7201 accgaggaga ctacgatcaa caggtataga agagagatct acgagcagtg cagagagaag
     7261 gcagccctcc ctccacagat gtttaccctg accgttccga ccggcggggg gaagacgctc
     7321 tcatcgatgg cgtttgcgct tgagcacctg aagcaacacg gtctgaagcg gatattctac
     7381 gtcataccct acacgagcat catcgagcag aacgctgcaa agttcaggga gatcttcggg
     7441 agccggaatg tgctggagca ccacagcaac ttcgatccga agaccctgtc ttctgatggc
     7501 gcagacccgg tggatcaact cctcgaactc tctgccgaga actgggacat gccgatcatc
     7561 gtaacgacaa acgtccagtt ctttgaatcg ctcttctcaa acaagcgatc gagatgccgg
     7621 aagatccata accttgccgg aagcgtgatc atactcgacg aagcccagat gctgccgaca
     7681 ggctacctga aaccctgcct ccaggcactc tccgaactcg tccggaacta tggttcaacc
     7741 gtcgtcatct gcacggcaac ccagccgaag atcggcgaac tcctggacga gagcatccgg
     7801 cccgtcgaga tcatgcgttc gcctgcagag ctttacgaag cgttccagcg ggtgcgggtg
     7861 aacgatctcg gttcattgag cgacgaggaa ctctctctaa ggctgcagga gcaccgtcag
     7921 gtactgtgca tcgtgaatac cagaaaccat gcacggcacc tgcacgacgc actctcggag
     7981 cacggcacgt gctatcacct gagtgccagg atgtgccccg tccacaggag aaagcacctg
     8041 aaggagatta aagaattact caagaagggt gccgactgcc gcgtcgtctc gacgcagctg
     8101 atcgaggccg gcgttgacat cgattttcct gttgtctacc gggcggtagc cggcgtcgat
     8161 tccatcggtc aggctgccgg gcggtgcaac cgcgagggga aggccgagcg gggcgaggtc
     8221 tacgtcttcc ggtcgaccga gcgatacggg caggctacca gctggcagca gcgtacggcg
     8281 gagatcggga ggatggtcat ggatacgtat gacgacccgc tctcccttcc ggctatcgag
     8341 gactacttca gaaggctcta ctcctacgag ggagacgaag ggcttgaccg gaaggggata
     8401 ctgccgtcat ttgagaggag atctggtgag cttgcatttc cgtttgaaga tgtcgggtgg
     8461 gagttcagta tcatcgagca gggcacgaag gacctcatcg tcccctacga tgaaaatgcc
     8521 agaacactca tcgatgaact caagacctgc gaatttccgt ggaagtatgc acggcgcctg
     8581 cagggataca cggtcaacat ctaccccaat gagttcaggg aactggaacg cgcaaacgat
     8641 atcgaccttt tcggcgatag attctatgtg ctgaacgata tcggcaaata ctccgaagaa
     8701 accggactta taaacagaaa ggataatgga ggggaagggg cactactgat tgtttagggt
     8761 ggtgacggca atagggtatg gaatacgact gaaggtctgg ggcgattatg cctgcttcac
     8821 ccgccctgag atgaagacgg agcgggtgag ctacgatgtg atgaccccgt cggcggcacg
     8881 gggtattctc gaagcaatcc attggaaacc tgccatctgc tggaagatcg acaagattca
     8941 tgttttgaac ccgataacgt tcgataacat ccgccggaac gagaggtcgg ggaagatccc
     9001 tgcaaataag gtgaaaagcg cccttcgcgg aggagacgtc tctctctttc aggattcgac
     9061 ggaagatgct gttcaacggg cttctctgtt gctgcgtgac gtctgttacg ttattgaggc
     9121 acacttcgag ttgaccgatg aggcgggtcc tgaagatact gtcgagaagc actacaatgt
     9181 tgcactccgc aggatgagaa agggccagtg tttccaccgc ccctatttcg gctgccgtga
     9241 atttcccgtt cagtttgaga tcgtcgaagg ggagatgccg gagtcctact acaccgggga
     9301 gaacagggga gagagggatc tcgggttcat gctctacgac atcgattttt ccgatgaaat
     9361 gaaggccatc ttttacaggg ccgtcatggt ggacggcgtc atcgacgtgc agaggtgcct
     9421 cggcttcggg ggcatatcgt gatcatccag tccctctgcc gctactatga cattctggca
     9481 gaggatgagg atgtagcaat ctcccggccg ggatatagta gtggaaaagt ctcgtttgcg
     9541 ctggtcctca gtccctacgg cgagttgacg tacatcgtcg atctcaggag cgacgacaag
     9601 aaaccgcgac ccagggttat ggatgtaccc ctgcaggatg tccgttcatc gggtatagca
     9661 ccctatgtcc tgtgcgacaa cgcgaagtac gtctttggtg tcgagaagtt caagcgaagt
     9721 gagtttgaga aaaagttcct gaagccttcc agtaagggca cgtctcccga ctacacgata
     9781 cttggcgaga atgaaaaaga ggtcgtatta attcatccac gatcacgaga ctgctttgag
     9841 gcattcaaag cccgccagca cgaggtattc gacagcctcg atgactccgg catgcgggca
     9901 ttcctcgcgt tcctggacac ctggaacccc gagaacttca gcaagcatcc gaagatagcg
     9961 gagtatgcgg atgatcttct cgctggtggg agttgtgtct ttgagtgcgg aggagtcttt
    10021 ctgcacggga aacctcccgt gagggaagtc tgggaaaagt attgctcaaa ccgcggagat
    10081 ggtgacagat ccactgccca gtgtctcgtg accggtcagt tggatacggt agcccgcata
    10141 catcagaatc tcaagggagt tgccggcgcc cagatgtccg gtgcctcgct tgtcagtttc
    10201 aacgatgagt cattctgctc atacggcaaa agaggtgaaa aacaagggct caacgccccg
    10261 atcagcgaat catcgatgtt caagtacacc actgccctga attacctcct tgcccaccag
    10321 gactaccgga tgcgaattgc cgatacaaca gttgttttct gggcagagac gagcggcacc
    10381 gcctgcgagg atcttgcaag tttcttcttc gatccccgtg aggagacagg ggaggatagc
    10441 agtgctgaat cccccggttc ggcgacagta cgggatctgc aaaagatcga gctgatcggg
    10501 gatatcctca gtaaggtcag gaaggggcag aagatccatg ccgcggatgt cggagcggat
    10561 ccggatacga atttctatat gctcggcctg gctcccaaca atgcgagact cgcggtgcga
    10621 ttctggcacg tagacacttt tgggaacttt atctcaaggg ctgcacgcca tcacctggat
    10681 atggagatcg tcaggggcga ttacgatccg cgatacgtgt cggtgtattg gctgctgaga
    10741 gaaaccgttg cgccaagttc cgataacaag gcagcctcac cgctccttgg cggcctcctc
    10801 atgaggtcga ttctgaacgg cagcccatac ccggtccaga tgtataccgc gatcctcaac
    10861 cgggcaaagg ttgaccgatc gattaattct atcagcgcgg gctttatcaa agcctacctg
    10921 ctacggcttg caagagccgg ttcgatgaat ttaacggagg atctgattac cgtgagctta
    10981 aacgaagaga acccgaatgt gccgtatcgc cttggaaggc tgtttgcagt gctcgaaagg
    11041 gcacaggccg atacgaacag agagatgaag agcacgatca acagcaagta cttcagcagc
    11101 gcgtcgacga cgccggctgt ggtcttccct gttctgttga aacttgcaca acaccatata
    11161 gccaaatccg actggggctt caagaccacc cagaatatcg aagagacatt gatgggagtt
    11221 gatgagttcc cggcatacct gagcctggag gagcagggga tgttcatgct cggctattac
    11281 caccagcgga aggcgttttt caagaagaaa gaggaagttg caagcgtggg tgaatgatta
    11341 tgagcgaacc aatcaaaaac cggtatgagt ttgtactgtt attcgatgtc gagaacggaa
    11401 acccgaacgg cgacccggat atggggaata tgccccgggt cgatccgcag accggtcacg
    11461 ggatcgtcac cgatgtctgc ctcaagagga aagtccggga ttacgtcgat ctggtgaaga
    11521 gcgacacacc cggctatgac atctacgtca agtccggtgt ggttctcaat gagcagaata
    11581 agaaggcgta tgactacgtt ggtatcgagc ccaccaacaa aaagccgaag gatagccaac
    11641 taaccgagtt catgtgccgg aacttcttcg acattcggac attcggcgcc gtgatgacca
    11701 cggaagtgaa ctgcgggcag gtccgcgggc ctgtgcagct caatttcgcc cgtagcatcg
    11761 acccgatctt ccagcaggag gtcaccatca cccgccaggc ggtcgccaat gagagggacc
    11821 gtgagaaagg ccagacgatg gggaagaagc agatcgttcc ctacgcactg taccgcgccg
    11881 agggctacat ctccgctcac ctggcccaga aaacgacggg attctccgag gacgacctcg
    11941 acctcttctg ggacggcctc gtcaacatgt tcgaacacga ccactcggca tcgcggggca
    12001 agctgtctgc ccggaaactc gttgtcttca agcacgattc ggcgctgggg tgctgccagt
    12061 ctcacatcct gttcgacggg gtcaaagtag agcgcgtcgc cggggatcta cctccgcggt
    12121 cattcgggga ttaccgggtc accgtcgcga aagatgtgcc cgccggcgtt gaggtaatcg
    12181 aaaagttgtg agtacggtgc gatataccga cgaagagctg ttggccctgt cgggcatcca
    12241 gcacatctgt ttttgccggc ggcagtgggc gttaatccac gtcgagcgcc agtgggagga
    12301 gaacctccgg acggcagaag gccacctgat ccaccaacgg gtggatgatc cgttcttcac
    12361 cgagagccgc ggcgacgtca taatctcgcg ggcattccct cttgtctcgc acgcgctcgg
    12421 cctatacggc gtggctgacg tgatcgagta catccgctct gagaacggca tctctctccc
    12481 gggccatgaa gggttctgga cgatgaggcc ggtggagtac aaacggggaa aaccgaagat
    12541 cgacgagcgt gacgaggttc aactctgcgc acaggcgatc tgccttgaag agatgttcga
    12601 tgttcacata ggccggggag atttttacta caacgaagtc cggcgaagag tgccgcttct
    12661 gctgtctgct acattgagag accgggttta ctctctctcg gaggagatgc acgatctctt
    12721 taaacagggc gtcacgccct ctgccgatag ttccaagaag tgcaatctct gctctcttca
    12781 gaacgtctgc atgcccaggt tggcgaagaa aaatctatcc gtccgaacat atctgcggaa
    12841 acacgtgaaa gaggcctgtg tagaggatgt ttaagcattg aggggattat agcagagttt
    12901 atatctccgt acacgtaccc cttccatcag gacacgctgc gcatgaggaa actgctgaat
    12961 acgttatatg tgaccacacc ggagtcgtat ctcggcagcg acggagagaa tgtcgttgtc
    13021 agggtcaaca acgaggagaa attccgtatt cctatccata atctcgaggg tatcgtctgt
    13081 ttcgggtaca tgggagcgag tcctcatctg atgcggctct gttccgacaa caacgtcggc
    13141 ctgtcgtttt taacgccgca cggtaaattt ctggggcggg tcaacgggcg ggtacgcggc
    13201 aatgttctgc tgcggcggac acagtatcgg agagcggata gccgcgatgc gtcgctggag
    13261 attgcgaagt cgttcatcat cggcaagatc gtgaactgtc ggacggttct cgggcggggc
    13321 atccgcgatc acggcgctgt catcgactgt aataaggttc gagcggtgga tgctttgctg
    13381 atcgagaacc tgttaaagat cgagggctgt gattctgccg atacactccg ggggatcgaa
    13441 gggaattgtg cgaaattcta tttcattacc cttgacgaac tgatcctcaa gcagaaggat
    13501 gacttttacc tggatgaacg gaatcgaagg ccgccgctgg ataacatgaa cgcactgctc
    13561 tcgtttttgt atacgctcct tgctcacgat gtcgagtcgg ccctggagac ggtgggcctt
    13621 gacccctatg tcgggttttt ccataccgac aggcccggac gagcgagcct ggcgcttgac
    13681 atgatggaag agctgcggcc gtttatggcc gatcgccttg tgctaaccct tgtgaacatg
    13741 aaacaaatca acaaagacga tttttttaag aaggagaacg gcggggttat tctgactgaa
    13801 acaggaagga aagaggtcat cggagcctgg cagaagcgta agcaggatac gattacacat
    13861 ccgtacctga atgagaagat cctcattggc ttgcttccgt atgtgcaggc gatgctgatg
    13921 gcccgtttcc tgcgggggga tatcgacggc tatccaccgt tctttatgaa ttgaggtgtt
    13981 tgagatgatg gttctggtca cgtacgacgt caatacggaa actgccgacg gaaagaaacg
    14041 acttcgcaaa gtggcgaagg agtgcgtaaa ttacgggcag cgggtgcaga actctgtttt
    14101 cgagtgcctt gtcgatcctg cccggtttgc aaagcttaaa cattctctat gcggaatgat
    14161 cgatgaagag aaggatagcc tccggttcta ttacctggga aaaaactgga aaaaccgggt
    14221 ggagcacttc ggtgcaaagg aaggctacga tccggagggt ttattagttg catgactctg
    14281 cgaacccctg gtgaacagtg attcccccgg gggttcgcgg agaaaacttt caaactctat
    14341 attattctta tggtctggtt cccctgtttt tcgtggtgag tgtttgccct gaataggggg
    14401 ccgcttcggg gattgtatgt tgtcattaat ataattgtgc cgatatttta tctgatttgc
    14461 tcattttgtt gaaaataaca tttatcatcc tgctgattga atatcaatag attcgggatc
    14521 atctcgcctc ctttgaaaag gtgaggcgct gagccggatc gttttgcggt cactcccctc
    14581 gcgggagtgt ggattgaaat tcctgtgata cggtgtaata ccaaaacata taaagtcact
    14641 cccctcgcgg gagtgtggat tgaaatctaa tcctgcattt ggaactgctg cccatagcca
    14701 cgtcactccc ctcgcgggag tgtggattga aatctccagc agtaccggaa aaaggagatc
    14761 gtgcaagtgt cactcccctc gcgggtgtgt ggattgaaat tt
//
LOCUS       DAJB01000065           12781 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_54618, whole genome shotgun sequence.
ACCESSION   DAJB01000065 DAJB01000000
VERSION     DAJB01000065.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_54618"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2327 a         4094 c         4221 g         2132 t
ORIGIN      
        1 tgcaccttct gccaggtgcc ccggttcttc gggagggcga tccggagccg gtcgctcgcg
       61 agcatcatcg aggaggtgaa ggctttttcg gcacacgggg ccacaaggct ctcggtctcc
      121 ggggggaccg ggtcgctcta cggctccagc ggcggggaga tggacccgga tgccttcatc
      181 gccctcctcc gcgggatggc ggaggtgatg gggcaaaaaa acgtctcctc cccggatata
      241 cgtgtagact gcatcaccga cgagatcctg gacgccatcc ggcagtacac catcgggtgg
      301 gtcttcttcg ggatcgagtc ggggagcgac cgggtgctcc ggctgatggg caagggcgcg
      361 gccgtccggc aggtcgagga ggcggtggag cggtgccggg agcacggcct ccgggttgcg
      421 ggaagtttca tcgtcggcta cccgggagag acggaacggg actacgaggc aacgaaggac
      481 ctgatcgctg cgctctcgct cgacgacgtc ttcgtgagca gcgccgagcc catcccggca
      541 acaccgcttg ccgacctcgt ggtcaggaca ccccgcgata agaacccggc attcgtgccg
      601 cacaccgggg aataccgggc gctcgatctc accgagagcg aggcccggtg tttcgacctg
      661 atgatgcatg ccgatatgtt ccgcccgcag ttacggctcg tgaccgacga ggtctacgcc
      721 gcttatctcg ccgaggcaaa gaagcagggg agggatatca gggcggcgac cgaacttatc
      781 ctccggtact cccgggcccg ggcgtaagag ccgggccggc aacctctctc ctctcttcgc
      841 cggggccccg gatctttcct ggttcgccag aacgggaaac ccttaagtat cctcgatcct
      901 ctactccccc tgccttgaag gggtaaccct tcgcaggtaa acaagttgga ggcagatacg
      961 atggctgaga gacagacagg gtcggtgctg gagacccggc cgagacggga agagacagtg
     1021 aacctgatgc gcgcacacga catcagggat atcaaggcac ggaacccgga aggagagagg
     1081 ctcggtgata tcagtgatat cgctatcgac cgcgacagtg gccgcatcgc gtatgctgtc
     1141 ctctcctacg gcgggatcct cgggttcggc gagaagcact tcgccattcc ctgggaggcg
     1201 gttcgcaccc gcccggggga gagggtcgct gtcgtggatg cggataagcg gacactggat
     1261 agcgctccag ggttctccag ggaccggatg ccccgggaag gagactggag cctgatccgg
     1321 acacccccga cacgtggagc aatggagccc ccggccccgg tagcagagag agaagttccg
     1381 ccgtcacgag agacggagcc ggcagcaccg gcggagtcca cgcttgcaag cgcacagaga
     1441 aatgtcccgg tgccgccgcc cgtccagacg gtcgcgggcg gctggggagg gcagccgacg
     1501 gggcggcgtg ccgaggagcg acctgttcgg acggaggccc caccccggga gactgtcgtg
     1561 gaggaggtcc gaagggagca gccggttgag atggagcggc ccgttacgac cccgggaccc
     1621 tctcccgggc acctctccgc ggccgacctg cagagttacc tcaagggtat ggactacccg
     1681 gcaggcaaac aggacctgat cacccacgca cgggggcgca gtgctccgga ggacgcgatc
     1741 gcggcgcttg aagggttcag cgaccggacc taccagtccg ccgccgacgt gagcgtggag
     1801 ttcggcaggg agagccgtgg cgaacagccg attgagacgg aaacacggcc cggggggacc
     1861 atcgtagagg aggttcacaa agagcccccc gttgcccgga gggcggcacc gtccctcgcg
     1921 cacctctccg cggccgacct gcaggtctac ctcaagggca tggactaccc ggcaggcaaa
     1981 caggacctga tcacccacgc acgggagcgc agtgccccgg aggacgtgat cacggcgctc
     2041 gaacagttcg gcgaccggac ctaccagtcc gccgccgatg tggggacgga gttcggcaag
     2101 atcaagtgat ggagcggatt cccccgctcc gggcggcagc cgggaaacac ccctcctgcc
     2161 ctcccccaca cgctctttcc gcggtggaga aatccccgct gatgcccggg catcggagcc
     2221 ggggagatct taaatagtcg caatacgcat cccccgctct gcgcgaaggt gaagatcgcc
     2281 ttcggaagca taccagggat atggtggttg caatggctga aacaattctg gagcagcagg
     2341 tacggaggac ggaggggcag gagtccttct catccgaaga tgttgtgggt aaacgggtga
     2401 agaacccgga gggctacgac ctcggtgaga tctcaagcct gcggatcggc ctgcccacgg
     2461 gaaaagtggc gtatgcggtg ctcaagaccg ggggtgtctt cggtctcggg gcgaagttct
     2521 tcgctgtccc catcgaggcc atggtctacc ggccggggga tgatgtcttt gtggcgaaca
     2581 tcagcaagta taagctcgac aacgagcccg ggttctcgga gggggactgg ccgcgagaag
     2641 caaactggag cctgatcgaa tcgaggcggc ccatgggccc cccgagccag gaggaggccc
     2701 gggccgtgac gcggacggaa gccccacccc gcgaggtcgt cacgacagag cgcgtggagg
     2761 tgcgggaggg gatgccgata cgggctgaag aggtggaaga gggcagggcc aggatggcgg
     2821 taccggtgac ggagcccgca ccgcacgaga ccgttacgac ggaacgagtg gaggtcaaag
     2881 agaagcccgc aacggtggag gaggtggaaa cccggccggt gacggaggct cacgctccga
     2941 cgctcgagtc catgacgagg gccgagnnnn nnntcggccg ccgacctgca ggtctacctc
     3001 aaaggtatgg actatccggc aggcaaacag gacctgatca cccacgcacg ggagcgcagt
     3061 gccccggagg acgcgatcgc ggcgatcgag ctgttcagtg accggaccta ccagtccgcc
     3121 gccgatgtgg ggtcggagtt cggcaaaatc aagtaatctc actttttttc aggaaaccac
     3181 gtccgcccgc acgctaaaga acaagttcaa cctgcaccgt ttcccgtccg ccgaacgtga
     3241 ggtagcgccc ggcagccctc cccctgatcc cggcccgccc gagatccgca agccccgtga
     3301 acggtcgctc tctccggcag gagaggatcc ggtccgcccc cttcggccct atcccgggaa
     3361 tgcggaggag gtcgcggcgc ggggcgaagt tgacgttcac cggccgtttc ccgtccgaaa
     3421 gggcaacttt cgggtcgcga ttcgcaaaaa acccttcttc gtcgagggcc gcccggagct
     3481 cgtcggccat gacgccgtac tcccggagca ggtaatcggc ctgataccac cgcctcgccc
     3541 gccacgccgg ggtatcgggg tgcgacgcga gaggagtgcc cggcagggcg cgaaacgctg
     3601 agaagtagac acgggagggt cggacccgcc ggtactggtc ggtgaggcag gagaggatct
     3661 ccgcatcggt ctcgtccgcc gccccgacaa cgagctgggt ggtgtggctc ccgggcttct
     3721 cctccgacac ccaggcctgg cgcttcagga tatcctgccg gtagtccttc accccggcga
     3781 tgcccccgag acggtcggag gacgtcgcct ccaggttgat gctgatccgg tccgccaccc
     3841 gggcggcgtc gtggatatcc gcccgggtgg cgccggggag gaccttgagg tgcaggtagc
     3901 cgtcgtaacc ctgccgccgc aggagttccc ccgtctccac gagatcgtgc atcacgcaat
     3961 cgacgtcccg ggggatcccg gaactcagga agagcccccc aacgcgccct tcccgccaga
     4021 ggccaaggaa ggtaccggcg agttctcccg gggagaacga gatccgctcc cgctcaaggc
     4081 ggacggcgca gtaggcgcag tcgtatgagc aggtgccgtt gaggagcatc ttgagcatcc
     4141 gtccgcaccc gcccgaacgg gggttgtagg tgatgctggg agcgactgcc ggaacgccct
     4201 gattggtttc gggcgcggcc gagaggaccc tgatgcactc ccccggcctg cagaggtcga
     4261 accgcccggc agcggtaaga cggatgagtt tctgctccgt aacggacatg ccggaagcat
     4321 cggttcgcgg ggtgaagagc ctgccgcgcg gagggtgaaa gtgtttgagg aggatctgcc
     4381 tcccccttcc ggggaaagac atatggtata cgccggaccc tatcaccgcg aggagagacg
     4441 aagatggaga gtcggccgga gtatgatgcc gtcgttgtcg gaggcgggcc cgccgggagc
     4501 accgccgcct acctgctcgg gaaggccggg caccgggtgg cactccttga aaagcagacg
     4561 tatccccggg ttaaggtctg cgggggttgc ctgagccaga agtccgtccg gttcctcgac
     4621 cgggtcttcc acctgccgat cggtgccctc cggcgggagg ggctgatcga cttcaccggg
     4681 acgaggtatg ccctctacat cgggagcagc cgtatcctcg ccgaagacct tgtggaaccc
     4741 ctctacttca cccggcgaga gcgctacgac gcccatcttg cccggatggc ggcggaggcc
     4801 ggggcggagg tccatacagg cgtggaagtg gtcagagtcg accacgtccg ccgcaccgtc
     4861 acgacatcgg acggggaccg gtatgccgcc cgggtcctca tcggggccga tgggatccat
     4921 agccgggtcc ggcgctccct cccggagagc gtcgtcgacc ccgagcgatg gcgcgaaaac
     4981 ctcgggtggg cactggaact cgcgatcccc cgcaaggagg cggaggcact cgcgaaccgg
     5041 gacggtccga tccatctgga cgacaagctc gcgacaccct acctcgtctt tgccgcctgc
     5101 cggtggggct acgggtgggt cttcccgaac agagaggcga tcgtcgtggg gatcggcggg
     5161 ctgttgcgga tgaacggaaa agaccttcgc gaccgctttg aggagttcct cagcgccata
     5221 ggcctctccg cgttcagaga tcggaaaccg gcaggatatc ctctgccgtt cggcaactac
     5281 atcccctccc ctgcccatga tgggacggtc ctcgtcggcg atgcaggggg gtttgcaagc
     5341 cccatcctcg gcgaaggaat ctattatgcc caccggaccg cagaactcgc cgcccacgcc
     5401 gtcgagcgcc acctcacctg cgccgcgccg ctcgctgaga cctacaccac tctcctccgg
     5461 caccgcctca ttcccgaact gcgggcggag atggcgttgc ggaacttcct ccacgggagc
     5521 atggatgggt ggaggcgtat acctctcgag gtcttcatga aggcgacgaa caccctggtc
     5581 atcgatgcgg tgcaggggtc ccggtcgttc cggggatttt ggcgggacga cgaccttcac
     5641 tcagccgtct ggtgagaacg acctccccga aggggaggga gttcgagaaa atgcgaagca
     5701 ttttcgagta gcgactctcc cgcgggggga ggatcttgag caccgccagg tgcgcgtaac
     5761 gacgctccgc agaggaagga gttcgagaaa acccaccggg ttttcgagtc aggggatacc
     5821 tctatccgcc cgtacgtcca agggacctcg cgatgaaccc tccccgctac ccttccctct
     5881 acgaggtcaa cacccgcctc cggctccggc acctcgcccg tgagacaggc cgccacacca
     5941 cgctcgacga tatccccgac gtctggctca cggggctcgc cgggtgcggg ttctcctggg
     6001 tctacctcat gggtgtctgg gagaccggtg aggccgggcg ccgggtatcc cgctcgaacg
     6061 aggagtggct cgaggggtat cggagtctgc ttcccgatct ccgggaggag gatatctgcg
     6121 gttccgggtt tgccatcgcc ggatactcgc tccacccgga cctcggagat cccgatgggc
     6181 tttcccggtt ccgcgaacgg ctgcaccggc agggtctcct cctcatgctc gacctcgtcc
     6241 cgaaccacac cgccccggat catccctggg tgcgcgacca cccggactac tatgtccacg
     6301 gcacggagga agacctcgcc cgccggccgc aggacttcac cgctctcgat ctccccggcg
     6361 gccggaccgt tctcgcccgc ggccgggacc cctgcttcag cggctggccc gacaccctgc
     6421 agctcgacta cggcagcccg gccctgcagg acgcgatgat cgctgaggtg caggggatcg
     6481 cgaggcagtg cgacggcgtc cggtgcgata tggcgatgct cgtcctcccg gaggtcttcc
     6541 ggcagacctg ggggagggat acggagccct tctggccgag agcgatcgag gctgtccgga
     6601 acgagcaccc ggagttcctc ttcatggcgg aggcctactg ggacctcgag tggacgctcc
     6661 tgcagcaggg gttcgactac acctacgaca aacggctcta tgacaggctc aacacagggg
     6721 ccgggccggt gcgggatcat ctccgtgcgg agcccgaata ccagcgaaag tccgtccggt
     6781 tccttgagaa ccacgacgag cctcgtgccg cggcggtctt cccgcccgac cgccatcagg
     6841 ccgccgccgt cctcacctac ctctgccccg gcctccggtt ctttcaccag gggcagtttg
     6901 ccgggcggcg gaagaagatc tctgtccacc tctgccgggg gccggaggag ccgaccgacc
     6961 cgaccatcga agagttctac cgggggctcg ttgcctgcct ccgccttccg gcgttccggg
     7021 acggggagtg gcggcccctc gactgcaacc ccgcgtggga gggaaacccg tcccacgacg
     7081 gctacatcgc ctttgcctgg gaggtgagag aagaacgctt cctcgtcgcc gtcaactatt
     7141 caccagaccg gggccagtgt tacgtcccgc tcccgtgggc tgatcttgcg ggaaaggccg
     7201 tccggctcaa cgacctcatg ggaccggcaa actacgaccg cgacggaggg agccttctat
     7261 cgccgggcct ctacctggat gtcccggagt ggggctatca tgtctttgcg gtcgccatgg
     7321 aagaggggtg actgctcccc gtcagaccga cggggagagg ctctgcaccg ggaactgaac
     7381 ctcggtcagg agttcgtcct ccggcacctc gcaggggttg ttgaggtaga gttcccgcga
     7441 gggaccggcc ggtgcaaggc cgtgctcgcc catgtaagca aagagccgtt cgtaagcctt
     7501 cccgaccccg ggataggggc ccgtgtagag caccgaggcg aaccggccgc ccggaagggt
     7561 cacgacctcg acggcagttc catcgaggtt gacgggcccg acgatgggga gcgccacctc
     7621 gatatccgcg tccgtctccc ggtactcgtc ttcgtggcag atgaacatga tcggcccggc
     7681 cacctttgca gcgggcttcc gcccttctgc aggacagaca tgagcgcaga gttccccgat
     7741 cagtctcggg atcgcctcct ggtaaacacc ccgctcccgg cgggaaagcg cccgcatggc
     7801 agggatatcc ttgataacag gttcggtaat cgacatacgg aatcctccgt tcagcgggtc
     7861 ctgcgtccgc aggacctctg caatcgcatg cagccgcccg gcctcctgct cggttgcagc
     7921 aagacgccgc gcaaagagat cccggactgt cccggcatct ccgcgttccc gggctgcgag
     7981 gatggcttcg atcttggtga ggtcgaaccc gagccagagc aggtgactga tatgaatgcc
     8041 ccgctcaatc agggcgtagg tgtagtaacg gtatcccgtg cagagatctc ttgccgcggg
     8101 aacgaggagc ccccgctcat catagagacg gagcgctttc tgtgagagtc gggtgattcg
     8161 cgagaacgtg ccgataggga tctggtcgac tggcatggat acaatccacc tgttggcgtc
     8221 gccgggtaat agacggtcgg agtctccccg gggggagagt tcctgcccgg cagaatagtt
     8281 cccggttgga cggcacccgg cgtcaacctt ccggaaccgt ccgggtgggc caggcccgga
     8341 ggaagaggcc gggaccctca ccacttctgg atgagggaga ggagcatctt acggacctga
     8401 gtggtcagct cgcaggaggg atttatctca agcgccctgt cggcacacgc aagtgcgtgc
     8461 cggtacatgc cgagatggta gaggacgctg catttcatgc tccatccggc ggcatatcgt
     8521 ggatcgatcg caagcacctg atcgaaacat tcgagagctt tgcggtactc cccgagcgca
     8581 tacagggcac cgcccttcag gctccagacc cggacgctcc ccgggtcgtc ggcgatggcc
     8641 cggtcgtaac aggtgatggc ctcttcgtgc cggtgcccga ggaagtgggc gtagccgaga
     8701 tcagtccagg ccgccacgaa atgggggtcg atcgagagca ccttatcgta gcaggcgatt
     8761 gcatcgtcaa gctggcgcat cgcgacgaag atgccgctcc ggttgtacca ggcgaccgca
     8821 gatcccgggt tgagttccac ggcacggtcg tagcaggcga gcgcctcctc gagccggtta
     8881 cggcgccggt gcagcatccc ccgggagacc caggccggag caaaatccgg gtctatcgag
     8941 agcgccctct cccagcaggc cagtgcgtcc tggtcccgcc cgagaagatc gaggaagaca
     9001 cccttgttgt tccacatctt cacgttggcc ggatcttctt cgagcacccg gtcaaaacaa
     9061 cttatagcct cgccgaatcg tccctgctgg gcgagggaga ccgcttttct gctcactgtg
     9121 gcatcagtct ccggaccttc ccggttttcg ggatcacctc ttccgaagag ctttcctgta
     9181 atactcattc cggacattcc ccccagggtc acgctactgg tggcgggcat ataatataat
     9241 cgttcccccg ccccggatga tggcagagcg gaaaaagcgg tcttcagggg atgcctcccc
     9301 actcatcagg gaacgcgcgg accggtgagc cgtcgatcgg tatgattgtt ccgggggcac
     9361 ggtgccgcac cgccgggagt acccgcgcca cccgccctgc ccctccttcc cggcggagct
     9421 cggtatgcac ccgggcgtgg cagatgccgc agagggtgag caggttggcc gcatcgtcgt
     9481 ttgtcggatc caggtcgacg tggtggatgt gcaggtcctg ctctgcgccg cagatggcac
     9541 attgctgccc gtcacgttcg aggatcacat cacgcacctc tttccatcca cgcagggcat
     9601 acttctcccg cgtcctgatg ggagtcgcga accgctcgca gtaaaggtag cctgcggtcc
     9661 tcgcggcaaa agggcactgg tagcagagcc ggcggaaacc gttccgctcg ggatggtccc
     9721 cgcagagagg ttcctgcgaa cggtcaaacg cgtccaggcg cagctggagg taggtgctca
     9781 cgatcaaaag agggtaggtt gcaggatggg ataagggctg ggacgccctc atcccgcggt
     9841 tcacggcagc accggggcgg gtgttgtggg ctgtacggat gtcctgctcc ggtgcacgac
     9901 ctcaccggtc atgctccgga tctcgatttc gatcgccgcg ccgttcgagg gatccccacg
     9961 cggggtaact aactccatgg ggttactgcg ggagatattc tcgtagactg tgagcatgtg
    10021 tcgcgaacag aacagagtga tcctggtgcc ggatccttaa aacaggtttc gccgacgtcg
    10081 tcacttcttc tcctcttccc ggtaggggat cggcacgtag gcgaccgacg ggcggggcgg
    10141 ggcatcgcct gcggaaagag gtccatcaac cggtagatcc cggcgggcat accgggggtg
    10201 acggggaacc cgagatcccg ggcctgctcg agggtgatca ggtagtcgtg ggtccacgtc
    10261 ccttccgtca gcagacggga gaggcggtcc gcccgctcag catccatccg gtcccggagc
    10321 aggccggcta cgaactcccg catctggttc tgggccttcg ccgcgatgtc gaccagaatg
    10381 aaggtctcat cctcgatctc ctcgggcggt ttgagtcgtg ggactcgcag gatcgacgcc
    10441 gccgggtact ccccgatccg gggatcgacc ggaccgagca cggcgttctc atccatcgct
    10501 acctcgtcgg cagcgaggca gaggagtgtc ccgcccgaca tggcgtagtg agggacaaag
    10561 accgttacct tcccctcatg gcgccggagc gccatcgcga tctgctccga tgagaggatc
    10621 cggcctccgg gggtatggac gatgaagtct atcggcatct ccggctccgt gagccggatc
    10681 gccctgagga tctcctcgga gtcattgatg tcgatgtagc ggaagagcgg gataccgaga
    10741 aagccgatct gctcctgccg gtggatgagg aggatgaccc gggtccggcg ctccgcctcc
    10801 aggtcccgga tcgcctgcag gcggcggacc cgggtcatct gctgcctgac gatcggcacc
    10861 gcaaagagga ggagaaggat agcgaccagc agcacggtcc agatatcggg cataccggga
    10921 ccttggtgcg cgccccacgt aaagaaggtt cgcctcgcgg gacatctcag aagaagaact
    10981 catccatcga cgtctgccgg gtgcggccgc cctcgatcgc cgagagcctg accccgagga
    11041 ggcggacgtc cgtgccgttg aggaacggca ggagcagatc ggatgcggcc cgccggatca
    11101 ccccggggtc cgcggtgaac cgttccagcg tccgggaccg ggtgtgcgtc tgaaagccgc
    11161 gatacctgac tttgaccgtg acgttccggc agcggagacc gtcggcacga agtgtcccgg
    11221 cgacaacgtc tgcaagttcg acgagggtcc cggagaggac ggacgggtcg gcggtatcct
    11281 catcaaacgt cctctcccgc gaaacggatt tgcacccctc cctgccctgc acctccgcgt
    11341 cgtcgatgcc ccgggcgagg cggtgcatca ggacgccgtg tctcccaaac cgggcggtga
    11401 ggtcctggac atcgcggcat gaaaggtcac cgagggtcag gacccccatc cgctgaagtt
    11461 cctcgccggt cttcttcccg accccgggca tctttgccgc cggcaggggg gcgaggaacc
    11521 ccacgacctc gccgggagag atgaccgtca gtccatcggg cttctggtag tcggacgcga
    11581 tctttgccac gaccttgccc ggggcgacgc cgatggagca ggtgagtcct tcctcttccc
    11641 ggatctcccg cttgattgcg gcggcaagcg cctcagctgc cgcgaaaccg ccggcaccgc
    11701 tgacgtcgag ataggcctcg tcgatgctca cctgctcgaa ccgatcggca tgccgacgca
    11761 ggatcgccat gatctccccc gagacccggg cgtagagcgg gcggttcacc gggaggtaga
    11821 cgccgtgcgg gcagagggca tacgcccggg agatgggcat gccggagtga accccgtagc
    11881 ggcgtgcctc gtaggagcag gtgctcacaa ccccacggcc ggcccctccc ttcgggtcgg
    11941 cgcccacgat caccggcctg ccggcaaggg acgggtcgcg ccggacctcc acggaagcga
    12001 agaagctgtc catatccacg tgcaggatga tccggccccc ggtcgtcatt atatgaactc
    12061 ctctccggtc tcttgaggga gtgttgggag tccggcgata tagggggttt ggtggggggt
    12121 ggattgcgaa gatgccgggt gcaagttcca ggatcgccgg gcatcttccc atcttggctt
    12181 cagaaacatc taaaacccga aaacggcttc cgaggggata tcgcctttgg gggtggggct
    12241 gacggggagg ggggagcatc ccccctcccc tgtctctaac attaactccc catcaccccc
    12301 caccccgccc ggcctccggc ctcctccccc gcccctcggg gcgggggcag tacgtggcga
    12361 tatcccccac ggtccactgt atggggcatt ttccgcggat ccaacgtatc catctgtagt
    12421 agaaatccgt tttcgggctt taatcttcga gcctcatagc cgcctacatc tccaggcacc
    12481 ccagtatgga gcatctctgc aacaggggtt tcgcccggtc ggaaccaaac gccccgcctc
    12541 accccccgcg cgcctcccgg tgaccgggcc gggcacatcc ggggcgggaa tgccgggaaa
    12601 cgcaataaaa tcggataaaa caagcagaat ttgtgatacg aatttacgac atcatgatac
    12661 gtgggcgagt gagaaccact cgctccacct tctccgtata atcggcagga atgtttgcga
    12721 ttacggatat gaggacgtgc aagtgaacta ccccccgctt gcgcaggggg tcttcccgct
    12781 c
//
LOCUS       DAJB01000066            6513 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_57952, whole genome shotgun sequence.
ACCESSION   DAJB01000066 DAJB01000000
VERSION     DAJB01000066.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_57952"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1133 a         2117 c         1862 g         1399 t
ORIGIN      
        1 gaggttgaag agtgccctcg ccccgaacga cgatcgcggc tccctcccgc tccagggtta
       61 tcgtccagtg cggcatcgca cccctccggc actattcatc cccatctccg acaacctccc
      121 agcggccgcc ttttcggggc cccactcgtt tgagccgacc cgcctgcttg agctgccgga
      181 gttcgccggg agcctgcccg ggttccagac ctccaggcgg tctgcaaaga gcatcacctg
      241 gacgctgccg gtgctggtgt agtcgcggtg gacgacggcc tccgccacca cctcttttcg
      301 gatctcgtag cgcgcctgtg ggccggcctc gcgggcgttc aggttcttgc accgcgggca
      361 ctgccggggc tcgagcgcgg catccgccgg atcgggggcg tcgtcgatga acccggcatt
      421 ctccagaaac tttcgctcga catccgcacc ggacagatgg atatacacct ccggcatcgc
      481 gctgttcttc tcccagccgg ccacgagccg gagctccatc tccgagagcc cctgcttctt
      541 accgttgctt cgggtcaggt ccgtgagcct ggcgtggcgg atggcgtgcg ggtaaatcgg
      601 cttcgtcatg ccaagtttcc gggcgacgta cttcaacttg ttctcgaccg tccggatact
      661 catgcgccgg ggctcgtgcc tatacctccg ggaggttaca aaaagtggca tgttcgcgtc
      721 cgcacggagc gggtgcgtat tgacccaggt ctgcagatcc ggcacggagc tgatcaggcg
      781 gagccgccgc atcccggtct tgccgttcac gatcaccacc gcaccgtagc ggtcgaactg
      841 gacgtggccg atgttgaggc taaggagttc cccgatccgg gcgccggagt cccacatgag
      901 catgatcagc gctcggtcgc gtggcttgtc gcagacgtcg acgagccgga cgatgtcctc
      961 gcgggtgatc aactggtcga cggggagatg cttgcgcgga cgcctcatcc ggatgttgca
     1021 gaagagctcc ttctctcttt cagggacaag ccagcggaag aagagtttca actcgaggat
     1081 gtcgccctgc agggtgaatg gactgaccgt cgctcttcgc tcgatgaagt aatcttccag
     1141 ccactccgga tttacctctt gtgcatcgct gaatttgctc caggtgagga aggtgtagac
     1201 ccgccagacc ttcgtatcga tggtcttctg cttcagctgc ttgagcttga ggtggcgaat
     1261 gtacttttct atatacgctt tattgaccgg atcgaggttc ttcagtttcg ctatcacatt
     1321 cattggcaac cattctcccg gcgcggttaa aggtttaaac agcgccaagt gtatgtgata
     1381 ggtttagggt ccagtcttga aggggttcaa gggttcgaat cccttccccc gcattaccgc
     1441 ttcgggatat agttaactcg gctcatgttg tccctgcacg ggttgtgata tcagaaaaat
     1501 ctgatcctcg taccaggcaa gaacctcctt gttaaaaccg tagagccagc ccccgtctgc
     1561 gatcgtgtgg cgccgggaga atttgtttgc tgttgatgag gccagattag tgccttttct
     1621 ggataagaac ctccatactg gctcagatcc aggatcgaac aacataatta tcagtattga
     1681 acatgtatta aacacacatg gctcggtttc cggacttgtt ggccatcgcc cctgatctgg
     1741 gtcgcatctt cctcttcatg ggcctggtat cttgccttcc cctgctcatc ggcatcatct
     1801 acggggagta cggaatcctt ctgccgatgg cgtccgcccc ggcggctttc accgtgctcg
     1861 ggctcctcct cgcccgaatc cataaactcg aacgggaacc gccgctctcc atcgctctcg
     1921 gatcggtggc cctcatctgg cttgtcgcct cgttcatcgg cgctctcccg ttcaccctgg
     1981 ggatagggat gccgtatacc gatgccgtct tcgaggcaat gtccgggtgg acctccaccg
     2041 gtctatcatt gatcctcgat gtcgactccg tgccccgaac tatcctcttc tggcggtcgc
     2101 tcactcagtg gatgggcggc atcggtatcg tcgccttcag cattgccctt gcgagcaggt
     2161 ccagcctgac gatcttccgg ctctaccggt cggaaggccg gagcgagaca ctgatgccca
     2221 gcgtggtggc gactgcggtc cagatgtggc gcatctacct catcctcacc gtgatcgggg
     2281 tcggactcgt cctgctctcc ggcattccgc tctgggatgc tgtcaacatc tccatgactg
     2341 cgatagccac cggcggtttc tcggtgcaca cgcaggggat catgtactac cagaacccgc
     2401 tgcttgagat gctgatcgtt cccatcatga tcgccggcgc tctgcccttc aagatctact
     2461 acctgatgta cttcagaaag cagaccaacc tctttgagga cgcgcaggcg cgcctgctct
     2521 tcctgctgat cgcgttcggc gtcttcctcg tcacctacga tctcgtcttc ctcacgcagg
     2581 ttgccccact ccaggctctc cgggaagggc tattcatggt gacgagcgcc attacctgca
     2641 ccggatttaa caacaccaat ccgttcgcct ggtccagcgt cacggtgctc ttcctctccg
     2701 cactcatgct catcgggggc tcctcaggaa gcaccgccgg cggcatcaaa ctctcccggg
     2761 tgaacctcgg catcgagacc ctcggatggt ggttccgccg gttgtatacg agcagccggg
     2821 tactcacccc attccgtcac aacggaaaag gggtcgggct cactatcgcg gagtacgaag
     2881 tctccaagaa catgctcgtc atcgtgctct tcatcctgac catctttgtt ggttcgatcc
     2941 tgctcctcca cctcgatcct tcaccgggat tcgactcgag cgacgtgatc ttcgatgtgg
     3001 tctccgccat ctccaatgtc gggctgtcca cagggttcgt caacccggag atgagcatgg
     3061 ccgggaaatg ggccttcatc atgattatgt gggcgggccg tcttgagatt attccactga
     3121 tcgctcttgc gatgggactt tcccggaagg ttagccagaa gcggggttga gaaacgagtg
     3181 tcgagcctcg gtgctggccc gtgctatttt ggtgaaataa cgacgcttca cgatgcccgg
     3241 tccggacgga gcattgcggg gggagtgacg agaaacccgg gctccatacg agcggtctgt
     3301 ggtagatcca cagtaccagg tgannattcc tcccccggtt gaaaccgggg gcctccttgc
     3361 ccgacgtgct tatgactcgt atcgatgcag gcttgccctt atgagggacg agtgcccgcg
     3421 agctctctac aaatgcatcc tgcccatcga aacggatgat cctgatctct acaggcggca
     3481 cactcccttt tgccagtatc tcccgggttc gtgtatcgat gagcgcgtgg taatcgggat
     3541 ggacaaggtc gaagatgctc ttgccggcga agtcctccgg cgacgatgca cggaagagcc
     3601 ggaccgccgc aggattcgcg taggtgaaga tgtcatcctg ctccagcagg atcgcatccg
     3661 gggagagttc gaccaggacg cggtagcgct cttcgctctc cttcaggata tcctccgccc
     3721 tcttccggtc cgtgtcgtcg tacatatagc cccgggtccc gacaagttct ccggcatcgt
     3781 caaagacacc caccatgttc gcaacgatgt tgaccagccg gccatcccgg cggcgcagga
     3841 tcagcgcgtg atggtagagt gccctctcct tttggagcag gtgcaggaga tcggctctat
     3901 cccccggatc agggtagagc gactggatgt tgcttgcaag cgcctcgtca aaggagttaa
     3961 acccgaagat ggcaaggaag gcagggttac aggctaaaat ccgtccgtcg gcagagatca
     4021 ggctgtcgcc ggtgaggtcg tcccagaaga accgggcgta tttctcctca ctctcccgca
     4081 acgcatcctc catccgcttg cgctctgcct cacctcgtct gagctccgca ttctcctgtc
     4141 ggagaacctc gttctccgcc tctaaccgtg cgatacgttc ggcatccggg cggccctgcg
     4201 ccagagatcc catagcagga tcgcgtcccc tcttgttttc cagtggatgg tcaggctccc
     4261 gatgagcact ttcgcggcta caaagagtca ggggaaagat tggcgtgaat catccggcac
     4321 cgggtaaata gccgatattg cgagtccttc acaacaggat ccacccccgc ccggtctctt
     4381 cgcgtcccgc cggagtgaag gggctgcacc ccatctcgcc tacgccagtt cgtgcttccg
     4441 caggaactcc cgaacctgcc tcgactcgat ccagccccgg tcgaggtcct tgatgtagcc
     4501 gttgatccgc tcgacctgct cctggatcct ctccacagcc accccttcct cgagcaggtt
     4561 ggtcatgccg aggatcacct ggagcggctg gcggatatgg tccccgagga ccgcgaactg
     4621 ctcgatgttc cgctcgatct gctcgaacac ctgctgccgg agctgctcgt tctgtttccg
     4681 ctcggtgatg tccctgccga ccacgtgaac cccgatcacc gaccgatcgc ggatgatcgg
     4741 ggactcgttc agttcgagga atgccgcgga cccgtctttg cggcggaact caatctcgag
     4801 cccctcgaca ggctcgcccc ggactatctt cttgtgtccc tcctcccaca cggggaagga
     4861 cgaggggacg acgtagtcgc ggcacttgca gccgatgagt tcgtcggggg tatagccgag
     4921 gatcctcgtc accgccgggg acatgtaggc aatcccgccc tcgtggtagc aggtgtagat
     4981 catatcgaaa ctctgctgcg tgatctcccg gaactgggat tcgctcttct cgaggcactc
     5041 ccgcgcctgg aatcgctcca tcgttctccc gaaacgtccg gcgatagtgt ctatcagcga
     5101 ccgctcctcc cggaggaacg ggccttcgtc ctcttccggt ctctcgtgga ggtagcagac
     5161 ctccacctgg ccgaccgtct cggcgtggac gacgatcgga gatgcctgcc gccagggcgt
     5221 cacccggaac ccttccgtct caaactcgcg cccatacacc gttatcctga cgacggcatc
     5281 ctccggatac tgccagccgg aagggagcgc ccgggccact tcgcagaaga tctcgtcgag
     5341 cgagatcccc ggctgctcga tgatgcggga taccgcatac agggctgaga gttcttttat
     5401 ccgctcgttg aggtcgtgcg tacgcttccg cagggtatcc tccgcctgcc tgatctcggt
     5461 ggtatccctc gcaatgaccg cgacccgggt gacggcgccg cgcgcgtccc tgaccgggta
     5521 gaggacggta tcgtatcgtc gcccttccca tatatcctca aaccggatag gcttgcccag
     5581 atcgacggca tcgcgaaggc ggtccgaata cacccgggct atatccggag gaagtagttc
     5641 gaacgtgttc atgccgatga cgtcctcccg gtcctgcgaa aaccgccgga ccatcgtctc
     5701 gttggcgtcg ataacccggc ccccggcatc caggagggca atcatgtccg ccggggcgtc
     5761 gaggagggcg cgggcggtcg cctcgctctc ccggagttcc tccccggcgc gcttccggtc
     5821 ggtgatgtcg tagagctgct cgacgatgaa cgccacctcc ccctgttcgt cgaggatcgg
     5881 gctcgagcgg cactccaggt accgcccgag ttcgggcacg aacttctcga cgacctcccg
     5941 ctttccgctc tcaagcgcag tggtcgtcgc acagaccgca cagggcccgg tccgcccgat
     6001 gagttcgtag cacttccgcc ccttcgcctc ctccggcgtc atcccgagca gctcgtagcc
     6061 ggcacggttg taacgcacga tcgtatgatc cggcagctgg atgccgacga tgtcgccgag
     6121 gttctcaaag agcccctcga gcagggtgtt cgcctcctcc atgcggcgcc gggcctgctc
     6181 cttcccggtg acgtcctgcc aggcgacgac ggcgccggag accgcctctc cggtgatcac
     6241 gggcgaggag gatacagcga tgtggatatc ctcgtctccg cgatcacgga taacgaaccg
     6301 gcaggactcc agaacctctc ccctgagcgc ccggtgcccg ggaaggtcac cggaccggac
     6361 cggtccgccg tcgggatagc ggatcgatcc gtcccccgag agcccacgga cgatccggtt
     6421 ctcttcgccc ggacccgcca cgaccatccc gccgtcagcg tcgtagacga tcccctgcct
     6481 cccgatggcg gcgaggagcg tatcgcactt caa
//
LOCUS       DAJB01000067            7420 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_110776, whole genome shotgun sequence.
ACCESSION   DAJB01000067 DAJB01000000
VERSION     DAJB01000067.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_110776"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2275..2397
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4632..4656
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1399 a         2306 c         2304 g         1261 t
ORIGIN      
        1 gcggtctcgc aggcagagag cgatcgacaa gggggaaatg aatgacaaaa cacacgcatc
       61 tggcactgtt gtgcgcagtc gtcatcatcg ggctgctgat gccgccggtt atggcggtag
      121 aagttgcgcc gggggcatcg caggattacc tcctccccgg cggaaatgaa cacccggacg
      181 tcgacggcaa catgatcgtc tgggagcgca gcctgctgag agggccaaag gacatctatt
      241 ccatgaccac ttccggcagc gacatccgtt cgctcacgaa cgacaacgcc tcacaggaga
      301 ggccgtccat atcgggagac tacgtcgtct ggcaggacaa ccggagcggg gactgggata
      361 tctatctcta ctcgttctcg gagaacacga cctcacgact tacgaacgac acggccgatc
      421 agtggctgcc cgtcatccac gggaattacg ccgtctggta cgacaaccgt gaagggagta
      481 ccgacatcgt cctctacgac atcggcgccg gagaagagaa agcgctcatc gactgcaacc
      541 cggcgctcgg tactgacgtg accaggttca aacccacgct ctcggacgag tacgtcgcct
      601 gggaggagac aggtgcaggg atcctgctct acgagatcgc cacggggaca agccgggcgg
      661 tatcgccgag cagcatgccc cagtcctggc cctcgatagc cggggacgtc atcgcctggg
      721 aggactaccg gtggggagca gcgtgggagt ccgcgatcta cctccacagt attggcaccg
      781 gcgaagaact ccagctgacg acgcagagtt ccgagcaggt ctcgccggca ctcaccgaga
      841 gcctcgtcgc ctgggaggac aagcgggatg gtctgtggag catctacatg tacgacctct
      901 ctgacggagt ggaggagatg tccgtgccca ccaccggcgg cgagcagctc taccccgccg
      961 ccagcggcaa cacgatcgtc tggcagaaga accggggcga gaacgcaaaa ctctgtgtat
     1021 atacctatat tcccggcgac ccgacacaga cggtcacgga gatagagatc gaaccgtcca
     1081 cggccatgat ggagatcggc gaggaactga agttcaacgc cacctgttac gaccaggacg
     1141 gtaatgtcac acccggtctg aaggtcacct ggtcctgcag caacaccacc gtcggactca
     1201 tcgacccggg aggctacttc aacgccgctg ccgcaggcac agcgaccatc accgccgcaa
     1261 cggccggcgt ctcctcgacc gcgaacgtca ccgtcaacgc taccgagctg atccttgaga
     1321 gcatcacggt cacaccggcc acggccaccc tggagatcaa cggcacagaa cagttctctg
     1381 caaccgctct cgaccagttc ggcaacgcca tgaccggagt ttcggtcgcg tggacaagca
     1441 gcaacgagac ggtcgggacc gtcgatgaga acgggctctt caccgcgttt gccgaaggca
     1501 cggccgatgt cactgcaacc gccggaggcg tcaccggaag tgcggctgtc accgtcagtg
     1561 ccgaagaacc ggtcctcgat agcatcacgg tcgcaccgac cacggccacc ctggagatca
     1621 acggcacaga acagttctcc gcaaccgctc tcgaccagtt cggcagcgcc atgaccggag
     1681 tttcggtcgc ctgggcgagc gggaacgaga ctgtcgggac tatcaatgaa accggcctct
     1741 tcaccgctct cgccgaaggc acggtgacca tcaccgcgac ggcggagaac aaatccgctg
     1801 aagcaaccgt cacggtcacc gccgaagagc cggtcacgac aagcatcacg gtcacaccgc
     1861 tcacggccat cgccacggtc aacgacaccc tggagttcac cgcaaccgtc cgtgaccagt
     1921 tcggcggcga actgtccgac gtcgaggtaa cctggacgag cagcaacgag accgtcggaa
     1981 ccatcaatga aaccggcact ttcaccgccc tcgccgaagg tatagcggcc atcactgcaa
     2041 cagccgacgg ggtatccggg agtgcggcta tcaccgtcaa ccctgaagag cagaccgaac
     2101 cggtcctcac aagcatccgg gtcacaccgt ccggggccac cctcgcgatc ggcgacaccc
     2161 agaggttcat ggtgaccgct ttcgaccagg acggcaccgc catgcctgac gtcgaggtca
     2221 cctgggcaag cagtgacgag accgtcggca ccatcggtgc ggacggggta ttcannnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca
     2401 ccgcagcggg agacaacgtc accggaacct caaccgtcac cgtgaacagt gacgccccgg
     2461 tcctcaccag catcgccgtt acaccatcgg cgatcacgct cgccgccggt gacaccgaaa
     2521 cattcaccgc gaccgctctc gaccaggacg gcatcaccat gcctgacgtc gaggtcacct
     2581 gggcaagcag cgacgagacc atcggcaccg tcggtgcgga cggggcattc aacgcgcttg
     2641 ccaaaggcgc ggcgaccatc accgcaacgg cggaaggcgt caccgggacg gcggccgcaa
     2701 cggttaccga tgtctcatcg ggggtcgtga tctcaccgtc ggagatcatc ctggatgtcg
     2761 ggggctcccg gcagttcacc gcgaccgtgt atgaccctga aggcaacgtg acgcccggcg
     2821 ctgtggtgac ctggtcctcc gacgacgaca ccgtcggcac gatcaccgcc gacgggctct
     2881 tcaacgcgct tgccgaaggc acggcgacca tcactgcgtc ggttgacggg accgacgaga
     2941 ccgggaccgc gaccgtgatg gttcagtcga cgtcgtcggc acccggccgc atcaggatca
     3001 ccccgtccga gctcaccctg gccgccggca acaacatgac gtttgctgcc acggttctcg
     3061 accaggacgg cttagaagtg tccgacgccg aggtcacctg ggcaagcagc gacgagaccg
     3121 tcggagtcat cgatgcggac ggagtcttca ccgcactcga ggaaggcacc gtgaccctca
     3181 ccgcatcggc gaacgagacc tcgggctcag cccaggtgac cgttgagccc tcgctcccgg
     3241 ccccggcccg catcgagatc tccccggcca cggccacgat tccgcccgga gagacccggg
     3301 agttcacggc caccgtcttt gaccagcgcg acagcgagat ggactggatc agggttgcct
     3361 ggtcgtgctc cgacacggag atcggcacca tcgacagggc tggcctcttt ggagcgttcg
     3421 cagagggatc tgcggatgta acagcctcgg caggcggcgt ggaaggaaca gcggcggtca
     3481 ccgtctcggc aaccgttgta cctgaaccca cccccgggaa cggcggcggt tcaacgaacg
     3541 gcggcggggg cggcggtgac agcagtccct ccttctccgc agggatacgc gagaacctga
     3601 gaagcggcga gacgttcacc ttctcagaac tgaccaccac atcggtaagc agcgtgaacg
     3661 tcacggcagc cagcaccatc ccgaagatga tgctgaccgt gaagaaggca aaagcgccct
     3721 cggctgcgga gcctcccgct agcgacgtct acgagtacat cgagatcacc ccccaatggg
     3781 tgggcccaaa ccagatcggc aacgcgacag tcttctttag cgtccctgcc gactggcttg
     3841 aaagccacaa cgcgaccgcg gaggacgttg ttctcatgcg ctacgtcgac ggcgcctggc
     3901 agcccctcga gaccgaggtc ctcggcgaag agaacgggaa gtataacttc cgggcaacca
     3961 cccccgggtt ctccaccttc gctatcgctg cgnncctgag gagaccggca acgtaacgac
     4021 ggagccgacc accgtgccgg cgacgacacc ggcggcaccg ctcatctacg cgcccctcct
     4081 tgccccgctg gccttcctcc tctgggcgag gaagaagaac taaccccacc tttttttgac
     4141 gctgttctgt ggatcttccc gggataacat gcaggaaccg ttcgcgatac gggagagcac
     4201 gacctccgta cggctcgctg aagaccggat gagaagtgca cgccccggag ccttcaacca
     4261 tcgcgacgat acccgcttac cgggtaaaaa ttgccagggc gacgccggaa ccacggacgg
     4321 cgtcaggaaa ggggagagcc cgggggtctc ctactccacg acgaaatcgc cggtcatctg
     4381 ctgggggtgg acgtcgcacc ggaagaagta ggtgccgggc tcctccggag cggtgaaggt
     4441 gtaggtggtc tcggcaggcc ccgtgatggt ctcgcccacg aagatctcct cggctgctga
     4501 ggagtccgtg tagaccgcga agttgtggag gatgccgaca tcctggttgt cgaagtttat
     4561 cgtcacctcc gcacccgccg gcaccgtgat cgtgctcgca ttaaacgcga ggttatcggc
     4621 ggcgaggtcg annnnnnnnn nnnnnnnnnn nnnnnncggt cggggtcccg gttccggtcg
     4681 gctgggtgac gttcccgccc ggcgcggtgc accccgcggc cagtatgccg gcgcccacga
     4741 cgaggagcgc caccagaagg accaggttgg gcttcatgag ggaggcgatc ctgtacatgc
     4801 tatttatatc tatccgcgcg ggaggtctgc acaggagaaa agggtgggaa gaacgggaca
     4861 atcccgccct tcgcacaccg gttcgttcca ggtcactcca cgataaaatt gccgttcatg
     4921 gagggatgga cgtcgcattg gaagtagtag gttccgggct cggacggtgc cgtgaaggtg
     4981 taggtgatct cggcgggccc cgtgatgatc tcgcccacga agatcgcctc agtcctgaga
     5041 gggccgtcgt agaccgcgac gttgtggggg acgccttcat cccggttgtc gaagcccagg
     5101 gtcacgttcg cccccgccgg caccgtgatg gtgtccagat cgaaagcaat gtcctgtgcg
     5161 gtaatattca ccaccgtctc gttcccggcc gtcgtcgggc tcggcgtcac gttccccgtc
     5221 gcggccgggg tcacattgac cgtcggggtc aggttcatcg tcggagtggg accggcaccg
     5281 gtgggcgccg cgaccctcca gaccctgccg gtgctggtgg acggatcggg ccctcccaca
     5341 tcgttcgtca gcacatagag ctcacgttcg gcatcctcgc cgaacccgcg caggaacatg
     5401 cccagggtct cgttctcgcc ggaggtgatg ttcaccatct ggacctccca catggcgttc
     5461 gcctcggggg tcaggttcgc ggcggactgc ggtgcggccc atcccgccgg gggtttggcg
     5521 gcgaggagag tgccgttgcc caccgtccgg ctgtcgctcc agtaaccgaa gacgtacctg
     5581 ccctcaagat ccggaacggc ggtcccgcgg tagacgttcc cgccgacgac gacgatgccg
     5641 aggtcgcggc cgccctcgaa tatggggccg atcagcggct ctcccaggta gccggtggtg
     5701 ttgcaggact ccggcggggc cgtggtggcg ttggggtcga agcagtgcgt gccctcgcgg
     5761 atgttccagc cgtagttgcc gccgttgtag acgacgtcca cctcctcaaa gaggttctgg
     5821 cctgcgtcgg cgacgaagag cgcgttgttg ccgccggagt caaacgacat gtaggccggg
     5881 ttccggaacc cgtaggcgta gatctcgggc gggacagcat cgtaggcgta ggtgtcgagg
     5941 atctcggtct ggttctcagc aaacgggttg tcggcgggta tgccgtagag gctgcctgca
     6001 aacgtcgtcc acgtcggatc gggcgggttt gcggtccggt tgacgctcat gttctccgtc
     6061 tggttcatca tcccggtcac gttctgcggg atcgttacgt tcccgtcggt gacattctcc
     6121 acgtcgatcc ggagcatctt gccgtagatc ttcgtgaggt cctgggcgtt cccgataccg
     6181 ggggtgtggc cattcccgac gtcgtttgcc cttccagcat ccccgagcga gatgtaaagg
     6241 tagccgtcgg cggggctgaa ggcgagctgg ccgccgttgt ggttatggta gggcttgtcg
     6301 atgtacatca gcaccttctc ggaggtcatg ttcacccggt tttggtcctc ggggtcgacc
     6361 tggaactcgg agatgtggtt cgtgcagttc cagtcctcgg gggcctccgg ccttaagggt
     6421 gcgctgtaga aggcgtagac cttgccgttc tccgagaagt tcgggtggaa cgcgatcgag
     6481 agcagaccgc gctcgtcgta ggtcgggttg agatcgacca tcctgtcccg gaggtccagg
     6541 aacggctcgg gaagcgtggt cccgttcgcg tcgacaacct gcaccacacc gatctggtcc
     6601 acgacgaaga gccttccggt cccgtcgtcg ggcgccgtga tcgcaagcgg cgatgtgaag
     6661 ttctccgcca cgagttcaag gccgatctcg gccgtaacgt tcgagaccgt caggttcgtc
     6721 cgggacgtgt tgccctggag ttccgcggca agcatggacc cggcgttcgt aaggtccgtc
     6781 gcgttgccgc tcatgttctg cgcacttgcg ccttccatac cgatcgtctg gtttgaactc
     6841 tcgttgatat cgctggtgtt gctcgatccc acctgcgctg cacccgcacc cgcgatgagt
     6901 gcgaggatca ggagggatgc tatgacaggg gtaacttttg tcatgcgagg caggacaggg
     6961 tatccgggct gataatggtt acgccggacg gtgtcatgaa tgaggagagg gaagaccccg
     7021 gaggtattcc atccgggggg aatctactcc acgatgaagt cgccggtcat ctcctggggg
     7081 cggatgtcgc actggaagta gtaggtcccg ggctcttccg gcgctgtgaa ggtgtaggtg
     7141 atctcggcgg ggccgtcgat gatctcaccc acgaagatct cttcgaccgc tgaggaatcc
     7201 gtgtagaccg cgacgttgtg ggggatgccg tcatccatgt tgtcgaagtt caccgtcacg
     7261 ttcgcccccg ccgggaccgt gatggtgtcc atgtcaaact cgacatcctg tgcggtgata
     7321 ttcaccgtct cgaacgtgag cgggatgagc ggcgtgtggt cgttgttgac cagctggacc
     7381 gagaggttgt gctcgcccgc cgtcacgttc tcccaggtgt
//
LOCUS       DAJB01000068            2519 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_164895, whole genome shotgun sequence.
ACCESSION   DAJB01000068 DAJB01000000
VERSION     DAJB01000068.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_164895"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1889..2059
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2181..2215
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          402 a          772 c          723 g          416 t
ORIGIN      
        1 acgcactgcc cccgcccctg aggggtgggg gttacgggta actccttatg aggtgagaca
       61 ggggaggggg gatgctcccc cctccccgta agcccccccc aatggcgata ttcccacggt
      121 ccactgcatc ggttttgctc tcgtccctac tgttccgaga agcatgagaa tggctggatg
      181 gagcgcggga atcccggcac cgggtcacaa ccctctagcg tgagaaagcc cgaaccctct
      241 aaccgcagcc cggcaaagat ccagcctgaa tgtatccccg ctccgtttcc ctaccccacc
      301 ctgcagtgcc cgcccccgcg tttcgcgaag tactgctggt ggtactcctc ggcccgccag
      361 aacgtccccg cgggttcgat ggaggtgacg atggggcggc ggaacctgcc cgagcggccc
      421 tcctcctcct tcgatgtccg tgcctccttc tcctgctcgg gggtgtggta gaagatcacc
      481 gaccggtact gcgccccgat atcggggccc tgccggttct gggtggtcgg gtcgtggacg
      541 ctccagaata cgtcgaggag ggcccggtac gagaccgcgc cggggtcgta ggtcacctgc
      601 acgacctctg cgtgcccggt ccgcccggtg cagacgtcct cgtaggtcgg gttttcgagg
      661 gtgcccccca tgaaccccac cgccgtctcc acgacgcccg gcacgcgccg gaacgtctcc
      721 tcgacgcccc agaagcaacc tgcgccgaag gtggcgcgct cgaagtcctt ctccggtgcc
      781 atgacagatc cgccaggagg gggatctgtg aaaaaggtat cggcgtcccc cggggggcat
      841 actcttatct ccagtcggtc ccacgtacct tcatgctctt tgaatcagac gccgtagaga
      901 cggtcgcccg gctcatggcg ctctctgccc gcaccgcccc gaaagccaag ggcatcgacg
      961 tcatcaagac gacgatcgtc actggagagg agaagacggt acttgccgag gcgatgcggg
     1021 agtacgggga gcggcacgac acggcgttct tcatcaggga tgcgggaaac gtcgcggcaa
     1081 gcgacgcctg cctcctcatc ggctcgctgc tcgccgacgc cgtgggcctc gactgcggcg
     1141 ggtgcggcta cgccacctgt gcggagatgc tcgatgcgca gggacatccg ccggccacgc
     1201 cgttccacgg cccgaactgc gtcgtccgga tgacggatct cgggattgcg gtcggctccg
     1261 cggtaaagac cgcgagcctc cacaacgtcg acaaccgggt catgtacacc gccggtgtcg
     1321 gcgcgctctc gctcggatgg ctggaagggt gcggtgtcgc ctacggtatc ccgctccggg
     1381 cgtctggaaa ggatatcttc tttgaccgca cacgctaacc gagtaacaac atgcccatta
     1441 tgaagatccg cggaggggat cttgacctcg tcgagtacga gttcatctcc ttctcccccg
     1501 gcgaacatat caggccgtgc ggcctccaga aaggctaccg gctcgcgccg gtgaccggca
     1561 ccgtcatcgt cgaggctccc gcacggattc acctcaccgt gctcgacatg aaccggttct
     1621 ctcccgaccg tcccggggga gggggcatcg gctttgccat cggggtctac tgcaccgccg
     1681 aggttacgtg cacggcgtca gggctcgaga tcgactactc ccgcgagccg atcctccggc
     1741 acttcgtgga ggccttcaag caggtcgtcg gttatgaggg cggattccgg atcactgcgc
     1801 gtgaccacca gtacgagcac gtggggctcg ggtccacgag caccatcctg attgcggtcg
     1861 caaacgccct caacgtcgcg gtgggctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnng gtctacatcg ccattccgtc ttccgatatc tcgtcggcgg
     2101 gggagaagga gttcgatctc ctgatgaaca aggcccggac cctcgactac cgcgaccgcg
     2161 aactgaagtc ctacctcgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagat
     2221 ctaccgctac atggcggcgc tccgggacgc cggtctcgag ttcgtcggca tgagttcggt
     2281 cggcccgtcc atcgcggtca tcaccggccg gccggaggaa gaggtggcgg cgatcctcga
     2341 aaaggcgggg ctccgggtcg cgatcgcgac cgaggtcgac aacgaggggc tgaagatccg
     2401 ggtggaagag agggggtgag ggccgggagc ccaacatcac aattttctgg ctctgtagaa
     2461 cctctcccca tcagagatca tatcattgac ttccatccca aactattccc tcacttagg
//
LOCUS       DAJB01000069            2703 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_97781, whole genome shotgun sequence.
ACCESSION   DAJB01000069 DAJB01000000
VERSION     DAJB01000069.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_97781"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          482 a          835 c          876 g          503 t
ORIGIN      
        1 aagacctacg ccatgaccgg ctggcgggtc ggctaccttg ccgcaccgga ggactacatc
       61 cccgagtgcc tcaaggtcca ccagtacgcc caggcgtgcg cgacgtcggt ctcgcagtat
      121 gccgcagtcg cggcatacac cggcgaccag agtgcggttg cccggatgcg ggaggagtac
      181 cgggcgcggc gggaccttct ctacgacggg ctgtccggtc tcgggtttga cttcccccgg
      241 ccggaaggtg cgttctacgc cttcgttccc atggggcgaa cccttatcga gaaggccgtc
      301 gagaacggcg tcatcatcgt gccgggcggg gcgttcggta cgagggcccc cgactacgca
      361 cgcttcagct acgcgacgtc ccgggagaac ctctcccggg cggtcgcgag gattgggaca
      421 ctgatggagt gagagaagca tggtgaaaga gttactcagg aagttgtcgg acgcccacgg
      481 cgtttcgagc agcgaaggga atatcaggga gatcatcagg gcggaacttg ccgggtcggt
      541 cgacgagatc cgcgaggatt cgatgggcaa cctgatcgct acgaagcgcg gcgacgactt
      601 ctccatcatg ctcgccgccc acatggacga gatcggcctg atggtccagt tcatcgatga
      661 gaaggggttc atccgggtcg tccccatcgg cggatggttc ggaccggtgc tctactgcca
      721 gcgggtggtc ctgcacggga cgaaaggatc ggtcccggga gtgatcggtg caaagccccc
      781 gcacgtcatg aaggaagacg agcggaagaa ggagatcaag atcgagaata tgttcatcga
      841 cgtgggtgcg gcgagcgagg aggaggtgaa gaatctcggg atcgagatcg gcaccccgat
      901 caccctcgac cgcgagttca cggagctcgc cggcaaccgt gtcacgggaa aggcgctcga
      961 taaccgggtc ggcgtcgcga tgctcatccg ggccctgcag caggcgaagt cgccccacac
     1021 gctctacggg gtcttcacgg ttcaggagga gattgggctg aagggcgctc gcgtcagcgc
     1081 ttactccttg aaccccgact gcgcgatcgc gaccgacgtc accatccccg gcgatcaccc
     1141 cgggatcgag aagaaggacg cgagcgtcga gatgggcaag ggccccgtcc tcgtcctcgt
     1201 cagcgcgagc gggcgcgggc tgatggccga cccgcggatg actgcctggc tccgggagac
     1261 cgcggagaag aacggcatcc ccttccagct cgaggtcggg accggcggca acaccgacgc
     1321 gacgatcatc cacctcgagc ggggcggcat cccgagcatc ccgttctcga tcccggcccg
     1381 ttacatccac tcccccgtcg aggtgatcga catcggcgac ctcgaggcgg gcatccgcct
     1441 tctcgtcgag gcgctgaaga gcaagcccgc actctaaaga tccctctttt ttgaaatttg
     1501 ttgaaaatct gttcccatct tcatcctaac cctgacgacg ttggtgctgg gccggcttgc
     1561 ccatgggatc ggcctttttc ctcggacatg tccccatgca gtggaccgtg gtgacttgcg
     1621 caccttcggt gctcgaactc ccgccgtgcc gccggtctcc atcttgtacc tactttttgt
     1681 cccccacccc gcccggcctt cggcctcctc ccccgccctt ggggcggggg cagtcatggc
     1741 gatgtgcaat ggacgggacg tccggagcat gagcaccgcc aggtgcgcag cccggtagaa
     1801 agccatgtcc ccagagtgcc cctggattgc gaccatctgg agcaaatagg tggagtttca
     1861 acaaagcctt ttttggcatt tccctgcaca gaaaggatta acgtatccac catgcagagc
     1921 ggtgcggtat gaccgacggc tggaccggat tcttacaggc aatcttcctc ggtgacccca
     1981 gttacctggt acggaccctg atcattggca tcctcgcgta tgttgccctc gtgatcatcc
     2041 tccgcctctc ggggaagcgg acgctctcgg cgatgaacgc cttcgacttc atcgtgaccg
     2101 tggcgctcgg ctcgaccctt gcgtcggtga tcacctcaag ggacgtctcg cttgctgagg
     2161 gggtcctcgc cctcggcatc ctggtcggcc tgcagtacat cgtctcctgg ttctccgtcc
     2221 ggtcggagcg ggtgcagaag gcggtgaagt cggagccccg cctcctctnn nnnnnctgcg
     2281 gtcggagggg gtgggttccc tcgagagcgt ggtcgctgtt gtcctcgaga cgaatgggaa
     2341 cctctcggtg cttacgaaga acaagagtga cgggatggga tcgctcgtcg atgtggcagg
     2401 gtctcgccgg tgacggatgg tggaaaattg ggtagtgccc tacgaaacaa ctgataatac
     2461 tttgttaaga tcccatccgg gtgctcccga ttgattgcaa tccggggcac ggctttccat
     2521 cgggctgcgc acctggcggt gctcgagctc cgttcctgtc ggaacgtcgc gtatcgccat
     2581 gactgccccc gccccctggg gtgggggagg aggccggagg ccgggcgggg tgggggttac
     2641 aggaaaccct tagtatgtgg agacagggga gggggagacc cctccccgtc agccccaccc
     2701 cca
//
LOCUS       DAJB01000070            6669 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_91164, whole genome shotgun sequence.
ACCESSION   DAJB01000070 DAJB01000000
VERSION     DAJB01000070.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_91164"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    639..651
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1713..1776
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1974..1994
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1274 a         2072 c         2138 g         1087 t
ORIGIN      
        1 ccgacgaacg caagcatcta tctcgacggg aacgacacag gagcccagac taatacgacc
       61 ctggaagcgg ttccggtcgg cgagcatacg atcgatcttg tgatgccggg ctaccggaac
      121 gcaaccagga ctgtcaccgt cgaggagggc gggcatatcg cccttgatct gcttctctcc
      181 ccggcaggcg gtgaaaccgg agcgaacacc accacaggcc gcgatggacc cgtatccggg
      241 cacccggcgc cgaacaaccc cattgaggct ttcatcgaag ccgtcctcac tttcatgaac
      301 gggttgctct tgttcttcgg catgggagag ggtatgatag ccccgcaggg gccggagagc
      361 accccggttc ccggcgaaga tgcgataacc cccgtgccga ccggcacccc tgtgctcccc
      421 gcaccgacgt tgggtgagac gcggagcatg aagaaccact ccggcgggct ctacatcgac
      481 tcgtaccctc cgggcatgac aatcgtcatc gacaacaaaa aactggtctg gaagacgccc
      541 caggtcgcct atggcctccg tgaaggcctg cacaacattc aggtcgaaga gagcgctcct
      601 gataggaaca aagaagagtc cggctaccgg tttgagacnn nnnnnnnnnn nctatcaggg
      661 cgagaagttc acggtgaacg ggtttttccc ggccggcacc atcccgggtg atgccggggt
      721 cgaaggcgta aagtcctgga tcaccgtctt ctggaacgga acctatcgct cacacgcgat
      781 cccctcgagc atcgtgaccg gtgagacgta cacggtagaa ccgctgagcg aagatgctgt
      841 ttcgatcagc gtcaggtcag gtcccgaagg tgccgcggtc ttcatcgacg ggttctcgac
      901 cgggaaggtc acgccatgca gggtggaagg cctctccccc ggccagcacc ggatcctggt
      961 ctcgatgccc gggtacctcc ccgccgagga ggtcatcacg ataccggagg gatcggacac
     1021 cggcggtgcc atcacctgca cgctccgcga gtatacccac gggaacctcc tcgtcgagag
     1081 cacggtcccg gacgcgaaga tctacctgta tggtcggtac accggggaga agacgccgca
     1141 caccttcgcc ggcatgaaca tcgggacgta cgaggtcagg gccgtctcgg agaatggttc
     1201 cgcaacggtt gaggacgtgc tggtgaggcc gggggagacg acccggcgcg tggtggtcct
     1261 ggaggatgcc ctgtgagccg gtgccggaac tcccgtttcg tggcggcggc tgccgtgctg
     1321 ctcgcaattc tcctccccgg ggcatcagcc gccgtcgtct ggggaccata cgtcacgaac
     1381 accagcgaga actcggcggt cgtcagctgg agagtcgttg aagaagcaga gggctgggtc
     1441 gaatacgcgc ccgagggcgg cgagacgtac cgcacaccct ctcccggggc ggggcccatc
     1501 caccacgtgc tgctcacggg ccttgcaccg gcgaccacct accgctatcg tgtcggagcc
     1561 gggaacgaga caacaggaga ctgcaggttt acgacgttcg gagacgaagc gttcacctgc
     1621 atcatatacg gcgatacccg ggctcagaag cccttcttca cccaggccga gcggcacggt
     1681 ctggtggcgg agaggatagc cgctgaggag gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg aggcgttctt cgccgtcttc
     1801 gatctgccgc cctactactc cttcgacgcc ggcagcctcc acgtgaccgt cctcgacagc
     1861 aacgaccggg catgggcgga tatggacacc cggaccgcat ggctggcaag cgacctcgcg
     1921 tcccccctgc cccggaagat cgcggcgttc caccacccgc cgttcagctc cgannnnnnn
     1981 nnnnnnnnnn nnnngcggcg tggacgccgc ctttagcgcg cacactcacg cctacgagcg
     2041 ctaccgggca ggcgggaccg aatacttcgt cgtcgggtgc ggcggggctc cgttctacgc
     2101 gctcgcggag gagaagcccg aggggtacca ggcgggccgc gggcagaccc tcggctatgt
     2161 ccgggctacc atcagcgctg atgcgatcga tcttgagatg gtggcggtcg ccgaggtccc
     2221 cgagggagac ggtggggtgg tgacgatgta tccggcaggg accgtcatcg acgctctccg
     2281 cctccccgca gatgccgcac cggagagtac cgggtggtgg gccgttctcg cggccctctc
     2341 cgacggtacg gcgctatcca gattacaggg atgataacta tgcgtacacg agtatcatgg
     2401 atgctgcttg cgctcctctg cgtgagcatg ctcgccgctg ccgcctcggc ggcaccgacg
     2461 accgaactgc acgttgtaaa gatagcagcc gatggggtta ccgtcctcga cgagacgacg
     2521 gtcgactacc gctggatgga ggcgaacctc ccggtcctcg gggacggcgt gacccattac
     2581 taccaccagg ggccggtctt cgagggggac aagtgggacc ctgaagagac gacgaacttc
     2641 aaggacaggg gagccgtaaa gggaacagac gtccgtgacc tcgccgaact ggtcggcggc
     2701 gccgaacccg gggacgagat catggtccgg gcggccgacg gctaccacgt cgagtttgcc
     2761 taccagaatg tctacgagcc cgacccccgc caggggccca tagggatctg ctggtacaac
     2821 ggcgccgaga caggggcagg cgagcggcag gggtgcggct acgtccccga ctattacacc
     2881 gggatgcgcc tcgtcttctt tgccgacaac tcgaccaacg cagaaggcct ccgtgtctac
     2941 gggaaccagg atatgtacga tacgctcccg gagtcggccc agcacttcta cgacctctat
     3001 ccttccaccg ggggtctcac ggtgaagtgg atagatgaga tccgggtcta caccggtggc
     3061 ttccacggcg agaagggggc ccttgcagga tctctcgacg gcccggcagc ggagacgacg
     3121 accgaaccgc cgacaacccc gctccccctc ggcgtgacgc tcgctgcggc cctcgctgcg
     3181 gcggcgctcc atgcccggag aggggcgtga tcccgatgca ccggggttat cagaccctcc
     3241 tcgccgtgct gatcggggct gcggccctcg catccgggtg tctcggcccg gctgccgccg
     3301 ccgacgaggt ggcctggaac ctgacgcttg ccggcgatcc ggagagagtg ctcacacatc
     3361 ccgagatccg ggcgctaccc gccgtcgagg gctacggcta ctcggtctcg acggtcggga
     3421 tcaagtacgg cccgaaccgc taccgcggcg tgctgctcac cgatctcgtt gagatggcag
     3481 gcggggccgg cgcagacgac ctgatctacg tctccgcaca agacggctac ctctgggtct
     3541 tcgatgccgc gcagttgaga ggagaggagt tcttcacctt cgacgagaac ctccacgaga
     3601 tccccgcacc gacgctccgc gtgattctgg cctacgaacg ggacggagaa cccctggcgt
     3661 acgaagacgg cgggccgctt cgcctcgttg tcatcaccga gagtcccgac gtcatcaccg
     3721 aaggaagccc ctgggtgaag tgggtcgacc ggctcgaggt ccacagggga tgacccggcc
     3781 ggtagtgatc ctcgctctgc tcgccctcgc tctcctcgcg gtgtcgggag gctgcaccga
     3841 taccggcccg gagcgcaccg tcctcacggt ggtcccggcc gggagccttc tcgccccgct
     3901 cgaggagata gaagccgcct tcgagagccg gcacccggag atcgacgtcc acatcgaagg
     3961 gcacgggagc atccaggctg tccggcaggt gacggatctc gagcgggcga tcgatgtcgt
     4021 cgcagtcgct gatgcctcgc tgatcccgga tatgatgtac atcccgatga gcgagggaga
     4081 agggaattat accgattggt atcttccctt cgcggagaac gagatcgtga tcgcatacac
     4141 cgaccggagc gccggcgcgg atgagataac ggcggagaac tggtaccggg tcctggcccg
     4201 tccggacgtg cgggtggggt tctcaaaccc catgctcgac gcctgcggct accgggcgct
     4261 catggtgacg gcgcttgccg gggagcatta cggtgagccc gggctctttg aagcgatcat
     4321 tggcggcgcg ttcgacccgc cgcttgtacc tgcccgcgca gagggcataa cgacgatcgc
     4381 cctgccggaa cggatgcggc cggcagacga gaaggtcgcc atccgggacg gaagcatcta
     4441 cctcctctcg ctccttgacg ccggcgggat cgactacgct ttcgagtacc ggagcgtcgc
     4501 cgaggagcac ggcctccggt ggatcgacct cccccccgag atcaaccttg gttctgcggc
     4561 gcacgccgac gattatcgga aggtgcatgt gaccctcggc tttgagcggt tccggtccat
     4621 aggaagcgag cggatcgggc agccgatcgt ctacgccgtc accattccca caaacgcacc
     4681 ccacccggac gaagcacaga tgtttgtcga ctttcttctg gacacgttcc gcgaagggag
     4741 ggccggatgg cccgtcccgg tgggagagga accggatgct gccatagcat accacgccac
     4801 ggattgacaa accttttccg caccgcacgc ccatgtagag gtagagtatg cccggcggtg
     4861 cggctctcgc aggtcaccgg acggccgccg ggattaccgc aaccggataa cggatgccgg
     4921 gcagtatgcg gccggcgaaa caccgatggg atactatgtt ctggaacaga gcgatggaga
     4981 cgatccggcc tgatgaactc gcggatcttc aactgaagcg actgaaatgg tcgctgcatc
     5041 aggcgcagaa agtagggcta taccagagaa agttgaagga agcaggtgtc gcctcagacg
     5101 acatcaggac gctcgacgac gtgcagaaac tccccttcac ctacaaacag gaactccggg
     5161 caggataccc gttcggcctc tttgcagtcc ccttaaaaga gattgtccgg atccacacca
     5221 cctccgggac caccggcaag ccgaccgtcg tcggctacac ccggcaggac ctggataact
     5281 ggtcggatct gatcgcgcgg aacatgacga tgatcggcct tggagaggac gatatctttc
     5341 agaacgccgt caactacggc ctcttcaccg gggggctcgg gttccactac ggcgccgaga
     5401 agatcggtat gaccgtgatc ccgagtgcga cagggaacac ccggcgccag atagagatga
     5461 tcgaggactt cggggtgacc gccatccact gcacgccaag ctacgccctc cacctcgccg
     5521 aggtggccga gtcgatggga aagacactgg ataccctcaa gaccgggata ttcggggccg
     5581 aaccctggtc ggagagcatg cgggtggaac tcgagcggcg tctcggcgtg aaggcctacg
     5641 acagttacgg gctctcggag atgtacggac cgggggtggc gttcgagtgc ccggagagga
     5701 acggccttca cctctggcac gactgctacc ttgcggagat catcgatccc gagactggcg
     5761 agcggctccc ttccggcgaa cggggcgaac tcgtcatcac gccgctcgtc aaggaggctc
     5821 tgccgctcat ccgctaccgc accggcgacg tgacccggct gatggaggac gagtgcgcct
     5881 gcgggcgcgg gtgtaagatc gagcggatca cgggccggag cgacgatatg ctggtcatcc
     5941 gggggatcaa tgtcttcccg tcccagatcg agcatgtgct gcgaaccctt cccgaagtcg
     6001 gggagcagtt catcgtatat atcgatcgcg tcaaacatct tgatgagatg acgattgagg
     6061 tggagatgag cagagcgggt ttctccggtg aactgcagga gctggcccgt atgcagaagg
     6121 gaatcgccgg cgagctccat aacacgctcg ggctccggac cgcggtgaaa ctggtggaac
     6181 cggggtcgct gccccggttc gaggggaagg ctaagcgggt catcgaccgg cgcggggcga
     6241 tctgatggcg ccctggaacc cacggatgga ggagataccg cccgatgaac tgcagcggct
     6301 ccagttcaaa ctcgtaaagt ccctggtcta ccggctttac agcttcaatg acttctaccg
     6361 gcgccggatg aaggggcagc aggttcaccc cgacgacatc aggacgcttg aggacgtcgc
     6421 gaagctcccg ttcatgtata agagggacct gcgggacggc tacccggata agatcttctc
     6481 tgccgaacag aacgaactgg tcaggtacca tgtctcctcc ggaacaacgg ggaagccgac
     6541 ggtcgtcggg tatacagagc gcgatatcga gaactggagc gagtccctgg cacgggggtt
     6601 ctcttcctgc ggcgtcgggc ggggggacgt gatccaggtc agttacggct acggcctctt
     6661 caccggcgg
//
LOCUS       DAJB01000071            4009 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_52304, whole genome shotgun sequence.
ACCESSION   DAJB01000071 DAJB01000000
VERSION     DAJB01000071.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_52304"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2331..2445
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          729 a         1318 c         1212 g          635 t
ORIGIN      
        1 actgtaccgg agtgctctgg gcaccctgat caacgccgat gtcaacggcg ctctgaacca
       61 tttacgaaag gtagccggtg attccgtgct tccacggata atcggtaggg gccgtgtcaa
      121 ccggcccgtg cgaataagga cgtcttttga accgtcaacc ttcgcaccga ttacattgca
      181 gccctgtgcc ccgcaagggg tcccgctgca agcccccggt tttaaccggg ggtagttgac
      241 accacgaagc gttcacgccg gatggaagag atcgatggca acacccaccg cacgaccgtt
      301 cctcaagtgg gcaggtggaa aggcgcaact gctcgatgcg ttcaccctga gagttccgga
      361 agaactcaac gagggaaccc tctcggtctt cgtcgaaccg ttcgtgggcg gtggagcggt
      421 ttacttccat ttcaacagca tcttccggtt cagggagtgc cacctcttcg atatcaacga
      481 ggaactggtc ctcgcctaca gcgtcgtgaa gaacgacgtt tccggcctca tcgactactt
      541 aagcggtctc tcggatgaat ttctatcaaa ggacgacgcc gggcggaagg agtattacta
      601 tgccatgcgg gatacgttca accgcgagag gggaggcatc gatttcgggc agtacggcga
      661 ggggtgggtt gagagggctg ctcaactcat cttcctcaac aagacatgct ttaacggcct
      721 ctaccgggta aactcacggg gcgggtttaa cgtcccgttc ggcaggtaca caaacccggg
      781 gatcctgcat gaaggtgtgc tccgggctga ttccgttgcg ctccggaata cgaccgtcca
      841 cctcggcgac ttcacgctct cggagtcctg catcgcagag gataccttcg tctacttcga
      901 cccgccgtac cggcccctga accggacatc gtcgttcacc cagtactcga agaacaggtt
      961 ttccgacgag gaacagaggc gcctggcagc attctacgca caatgcgacg ccagaggcgc
     1021 caggctgatg ctgagcaact cggacccgaa gaacatcgac caggacgacg tgtttttcga
     1081 tgatctctac gccggatacc acatagaccg ggttccggcg aggagggcga tcaactgcga
     1141 cgggacgaaa cggggagaga tccgcgagat catcgtcacg aactatgctc cgcacggtcc
     1201 ttcggggcga tagtctccgg gtttagggat ctctggcaac cggatctcct gggccatccc
     1261 aaaagtataa gtcctcacgg gagacatgcg ggatacatgg cattccggga gatagacaac
     1321 gacctctggg agatcgttca aaagcacctc ccaccccgga aaccgcatat agggaggcca
     1381 cgccatgacc cccgggacct cttcaacggg gtcctgtacg tcctctccac cgggtgcacc
     1441 tggagcgagg tgccggcgaa gtacggtacg aaatcaacgg tgcaccgcta ccacctcgaa
     1501 ctctgcgagc gaggggcata ccaggcgatc ttcctcgatc tgctcagagc cggctatgag
     1561 ttcaagaaag gggaggccgg ccggtgtgcc atagggaacc tccaggctcc cgtacagtag
     1621 gcgctaaaac tcccccgcca cgacaacgaa gaggtcgccc cgggccgccg gaggcagggc
     1681 ccccgtcccg acggcgatcc gttcctccgg gtagccgagg tcctcgcaga cggcaagccg
     1741 ggtctccggc gggagggcgg ccgcaagcgc cccgaccgag aaggccgggt cggcgatgag
     1801 gaagacgacc cggcccgcga gaacctcctc acgggtctcc gcgaccgccc gggcatggtc
     1861 cttcccgtgg gccgagacga ccgccacctt cgtcagcggg accttcaggc gggcgaaggc
     1921 gacctggagg gaggatatcc ccgggaccac ctcgcccgga aggtagccga gcccggcaag
     1981 catcgggtcc cccgtcgaga ggacgaccgc gtgagacggg agggaccgga ggctccggta
     2041 atccgcgatc tcgtgcacct cgcaccccgg cgggatggca tcctgcgcaa gcgcaatcgc
     2101 ccgggccgag ccgtagacga gcgacgcctc ctcgatgacg ctcgccgcct ccgcggtcag
     2161 catcccgggg ccgcagccca cgccgacgac cttcatggcg actccccgat gattcggccg
     2221 tcgcggtcga cgatcgcgac ccggacgcgg gggtagcgga cccggaacgc gcgaagttcc
     2281 cggcgggcca ctgcctccca cagcggagtc gccgagagtt cctccactgt nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccccc ggaagcccgg
     2461 gcgatacggt cgagcacccc ctccaccatg tgatcgtccc agggctccac gaacccggtc
     2521 gtcccgagga ttgaaagccc cccgaggacc ccgatcttcg ggttcagcgt cttctgggcc
     2581 acctcagccc cccgggggac cgtgaggatg accgtcgtcc cgtggaggtc cgcctcgtcc
     2641 accgcctcgt tgatcgagcg ccggatgcag tcgagcgccg gggcgctgat cgccggcgtc
     2701 ccctgcaggt gacgacgggt gttgcggccg aaactcccga taccctcccc ggggacgaac
     2761 tggaccccgc ctgagaggga agggaccgcg gtcgcgacga actcgagccc cgcggtcacg
     2821 tcggaggggt agtccccggc gtccttccag cacgacgccc gtccccggta ggcgtccacc
     2881 gggagcctga ccgcaatgcc gcagggaagg gtgattccca ccccctctat ggtatcgaag
     2941 gcgagcgaga ggaccgccgc cttgcaggcc gcagccgctg tcgtcccggt cgtgaatccc
     3001 cggcgaagca ccgatcccga cgccgtcagg actgcgaggc cctgccggac attgggaagg
     3061 aggtcggggg agcggcaggc tgccacccac gcctcgggat actcaaagtc agtaaccggg
     3121 tcccgcatgg cctgcacgct cggcgaggat cgtgatgatc tcgttcatcg ccgccaccgc
     3181 aaccggtgtt ccgccccggg tgccggcgtt ggataccgac ggcacgtcga gggcacggag
     3241 cgcctccttc gactccgccg cgttcacgaa ccccaccggg gtcccgatga ccagcgccgg
     3301 ccggactcct tcgcggacca tatcgcagac gacgaggagc gccgacgggg cgttcccgat
     3361 gaccacgatc gatccctcta tctggtcccg cagggcaagg aaccccgcgg atgtccgggt
     3421 gatcccgcgc tcgcgggcga tatcatcgcc gaaatcgagg gcgcagagga cctcgcttgc
     3481 atgcccgcgc ttcaggatgc ccgcctgcac catccggata tcgacgacga tcggcgcctg
     3541 ccgcccgagc gcagagagcc cggcctcgac cgggtcgccg gcaaaacgca tcaggccggc
     3601 catcgcaaag tcaccgaccg ctatcgagca ccgctgccgg acccggtcct cgggggtggc
     3661 gtttcccacc tgttcccggg ccaggttccg gctcgtgctc gctatcgcgt aggcctccgg
     3721 cgtgaccgcc gcgaggtcag tatacgtatt tccggtgata tccccggggc gtgatgattc
     3781 ctctcgcatc agtcttatcc ctccagatcc tcgtctcttc tccgccgaca aaaacgatcg
     3841 agtgcatgtc cacaaaggcg aagtggtctt caaactcacc gagcgtcgtt accagccgct
     3901 cctccccctc ccggtaggcg ttcctgacca ccccgaccgg cgtttcggtc ggaaggtggc
     3961 gacgggcgat cccgatcgcc gtattcaggt tgtgcggtct tccccggga
//
LOCUS       DAJB01000072           11400 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_31895, whole genome shotgun sequence.
ACCESSION   DAJB01000072 DAJB01000000
VERSION     DAJB01000072.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11400)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_31895"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1323..1387
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1992 a         3499 c         3538 g         2306 t
ORIGIN      
        1 tgccatcagg catcaggccg tagtaaaaga agtagcgcag gtcccaatcc tggcagttgt
       61 ggatgagcaa gccgtcggag gcgaagaacg tatggctctc ctcggtcccg tcgccgtcga
      121 ggaagaggtc gtagacgtac gcaccggttg gctccgccgg agatatctcg acgacggtct
      181 cctcccgctc tcttacctgt ctctccttca ccgcagagag gaaaccgtcg atgtggtcct
      241 gccgctcatc gaggaaggtg gcatatcggg caaacgtctc gatattcttc cgcccgttga
      301 gctggagccg gtaaagagtc gggcggtctt cctccccgta gagttcgacg acctgggggt
      361 tcatcccgag cgtggtgagc aggagagaga gttcctgtcg gagggtcggt gatatggtgg
      421 tgtaattgac gatgcagtcc gatttctccg gccggtagta tgccgagccg tctccggtga
      481 agagagcgga gaggaactcg tggaggagca cgtcgtcgag gtggtatacg atccagggga
      541 ggcgtttgcc tgccgctccc tccgggatgc cgaagacgta gcggaagagc agataggccg
      601 gtttcccacc gaagtagacc tgcagggccc ggttgtgttc cacctcgatc ccgtagatgg
      661 tcgtggtgtc gggagtgccg ccggcgatcg ttgcgtacgt gttcagggtg gtgcgtgccg
      721 aggccaggat ggcgggtgcc tggcggttct cggtgatcgc caggttgtac tgcccggcct
      781 ctaaagaaac gttgtagttg ccttccgcaa cgaagaaccc caggaggcgg atgagctcag
      841 gtgtcagcgt gagaagggcg ggcagttcgt gttcgctccc ggtgaccccg atggtgaccc
      901 cggcatagtc ctcgacacta tagcggctgc agaaggctcc aaagagagtt accggcatgg
      961 tcccccgcgt ataccactgc ttctggtgct tgacgccgag cgcctgcgag atctcggtgt
     1021 acgaggtggc ccgtccggtc cgcaccgcat cgataaagat ctcccgggca ccgcggacgt
     1081 agacattctc aaggagatgt gccggcaccg atgccggcag ttcccggacg agatcgattg
     1141 cccctactgc ctctccgcgg atggtatccg tcccggagat cctgtagaga gcgtcaccgg
     1201 cacgaacctc atcggcacgg cggatggtca tctcgccccc ggtgcggacc gggatccggt
     1261 gctcgccggt gaccgtcagt gaacgccccc ctgaagtccg gatccggagc atctcacccg
     1321 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnntcc atccgcacgg gtatcgctgt gctcccatcg atacagaatg ggcgggtacc
     1441 gaagtattcc aggtcgtgga tgtggagatc gccgcggagg tggtggtccg caagttccgg
     1501 ggggagctgc aggaggtact gctccttgct gatcttgtcc gccttcttct tgtgcgaggt
     1561 ctcggcgttc tcctggagat tggcgttgtc gtgggcctca aagcccctcc ccacatcgat
     1621 gaggtgcgcg tcgaagaccg gggttcccac ccgggtgcag acgttgcggt aggacgtaag
     1681 cccccgctca aggagcgtca tgttgacgat ctcgcggatc agggggccgg agagcgactg
     1741 gagccccagc atctgaatct tcctctcgac ccgccgggcg atatcctgcg ccgtctcctc
     1801 atcggctccc tcgtagccgt agaagacctc cacgagctgg ctctcctcca cgatctgcct
     1861 gacgatccga tcgcggttcc agtcgaggag gtagcccctc gaggtccgga ctttcgggaa
     1921 cgtggggagg aagtccccga atatggtcgt ctgcgttgac ttgcgtgtca acctcatgcc
     1981 tccgtgatca ggtcggcgac aaggtcttcc cggaacgcgc ccccaacgaa gagatcgccg
     2041 gacgtataga atgcgtcgcc cttctgcagc accggcgctt cctgaacgaa tatgccgttc
     2101 atgcggagtt ctgtcagccc ctcggcggac gccatgtcgc actcgacgaa gggaacgccc
     2161 ctggatgcca ggaactcttt gagtatttcg caattcgggc agaattctag tgtatataca
     2221 atgaattgcg ttgtatcgga aatgataata cccccggatt cggtcgttat gttttcgctc
     2281 tgtacggtta taacagatct tattccttac cgggcaccgt ttaccagtgg acgcgcaccc
     2341 ggtatgccgg agcggattgc ggggcgaacc cctgccgaat ttggccctga gccgtctccc
     2401 cgctccgttc tgccgggtgc gcctctgccg gacaatcacc attttgtagg gtgaggcacg
     2461 atagtacggt agtatggtca ggacgttcgt tgcaatcgat ctgccggagg agatccgcga
     2521 gagggcccgt gagtcccagg agatcctgag accgtctggt gggcggctcg ctatcgtcga
     2581 ccctgcgaac ctccatatca cggtgaagtt tctcgggggg gttgatccgg cacgaatcga
     2641 gccgatcgtc gaggcgctcc gggccgtcag ggccgacccc ttcgatatga cggtcaggta
     2701 tgcggtctgc aacccgccac ggaggccgcg ggtggtctgg tgcgacgtcg cggacgccgg
     2761 agagagcgcc gcgctcgccc ggcaggtgga cgacctctta gcgccgctcg ggttcccgcg
     2821 ggagagccgc cccttccgcc cccatgtgac ccttgcgcgg gtgaaagagt tccacccctc
     2881 gcagtgttcg caggtggagt gcacaccccg cgaaccgctc gggagattcc gggtggagag
     2941 catcaagctc aagaagagca cgctcacgcc ccgcggatcg atctacgagg atctggcgga
     3001 ggtcccgctt tgacccggtg cccctgtgag gaggaggtgc tcaagaggat ccgcccaaca
     3061 cctgaagagc ggacctatat ccgggcgatc gggcagcgcc tgatcgaggc ggtggagcgg
     3121 tcggggatgg cgaaggcgat gatggtgggg tcggtcgccc gcgacacctt cgttcggggc
     3181 gaccgggacc tcgatgtctt catgctcttc gaccccgccc tctcacggga ggaactgcag
     3241 gagcaggggc taaccctcgc acacaggatc gccgaggagt tcggggcgac ctggcgcgag
     3301 aagtacgcgg agcaccccta cgtcaacgca acgatagact cgctggatat cgatcttgtc
     3361 ccctgctacg ccgtattgag tgccaccgag atccagagcg ccgtggaccg gactccgttt
     3421 cacacccgct acattctctc ccacatcgac gactacgccg acgacgtcct cctcctcaag
     3481 cagttcacga aggcgggcgg ggtctacggg tcggaccaca tgaccgaggg gttctccggc
     3541 tacctctgcg aaatcctgac gatctactac ggggggttcc acgcgctcat cgaggctgcc
     3601 gcctgctgga agcccgggga gacgatcgat atcgagaagc atggaacgaa gcggttcgag
     3661 gactcgctcg tcgtcatcga cccggtcgac ccggagagga acgtggcggc ggcgctctcg
     3721 ctctcgcgga tgttcgagtt cgtggaactc gctcgcggct acctcgcgga gccgtcggag
     3781 gcgttcttct gccgggggca gtctcctccg ctcaccccgg agatcttttc ccgcctcctc
     3841 tctgcccggg ggacgcacct cttctccctc accttcgcga cgccggacta cacgcccgac
     3901 acggtggttc cccagctccg gaagtctgcc gagtcgatcc gagaactcct ggagcggagc
     3961 gggtttccgg ccaatcgcat cgacgtctgc atgacagagg agcggtgcat gctcctcttc
     4021 gagctgatgg ccggcgaggt tccggtgatg cgccggcatc tcgggccgcc ggtctcggcg
     4081 cgggagaacg ccgagaagtt cctcgcgaag tacgtccgtg aagaggttct cgccggcccc
     4141 tacgtcgagg acggccgcta cgtggtggag ctcccccgcc cgttcacccg ggctatcgac
     4201 ctgctccggt caaaagccct ctttgacgtg gcgctgggca agcacgtccg tcgctcaatg
     4261 aagcagggat ggacggtgaa ggctggtgcg gagtgctggg acgaggagtt tgccgggttt
     4321 gtctccaggt tcctcgatcg tgcatcaccg cttgcgagga tcaagaggac gacggggaag
     4381 tagggtgcct acggaaccac gacagcgttc tctgtcacgg ctatcctgta ttgttcgtct
     4441 tgaccgtagg ggttgacgag cgtggcacag agacttggcg tgacgaccgg cacgacacta
     4501 cgctctgtgc cccatgggcg gcgatgatga gcgggatggc aaaggtcgca atgctcccga
     4561 acgcggctgc catgaccccg gagttggtcg tcttcggcgg agcccgagtg ccccggcgca
     4621 gggggagcat aatcgggcac gatacgttga ctatcgggat gagtccccaa catcggtcga
     4681 atacgggatg gttcaaaggc ctttcaagtg ttccggggga gcctgcaccc ttctacgttg
     4741 tcaccggtcc ggacccccgg ctcatccagg gcggcctgcc ggagcgtgaa ccggaacgcc
     4801 gcccctcgct caggatggcc cggcaccctg tcctccgccc agattctgcc gccgtagcgc
     4861 tcgatgagta cctgcacgag aaaaagcccg agtccttcac cgacgcccct tctcctcttc
     4921 tcgtaccggt ggaacacctc ctgtttctga tcgtcgggga tccccgggcc ggtgtcctcg
     4981 accgagaccc ggacgaactc gtcttcgtcg tccacccgga tctcgatctc gacatcgggc
     5041 ccgccgaact tgacggcgtt gccgatgagg ttcgcgaaga cttccgggag gaggtcgtcg
     5101 gccagtaccc ggcggttgac cgcatcgtaa cggacgacgc cctcagggaa ctgtgcgatc
     5161 tcatcaagga taaccgtatc caggtccacg cacctgtgcc cgccggacgg ctggtgaatc
     5221 cgccggatct tggagacgtt acccaggatc tcgatgcttt tccggatact ccgctgaagt
     5281 ttctccacgt atgtccgcga atccccgtcc agggtctcga tgagcagatc ggtgtagagg
     5341 ttcgcgacgt tctcggtgtt gcggatgtcg tgggtgagga tatccaggta gaggttcgcc
     5401 tcgcggttcg ctgaggcgag ttgctggtgg agtccggcaa ggtcttcggt gtggcgctgg
     5461 agcgcttcct cagtctgttt cctctcggtg atatcggtaa acatggcaaa ggatcccgca
     5521 aacctgtcgt ctccatcgct cagggcggtg gcagagacca gaacccacct gacgctgtga
     5581 tcgttgtgcc tgagccggca ctcgtatctg ccgccaagac cctgctctct ctccgccatg
     5641 cggctccagt gcttgtcgag ttcctctgta actatgaagt cggttgccgg tcggccgagc
     5701 atctcttcaa ccgaataacc gagcatctcg gccatcgtat ggttgacgta ggtggtcgtg
     5761 tagcggctgt ctatctgcag gatgccttca ttcgccatct cgacgatgga gcggaacttt
     5821 gcctcactct tttgcagggc ttcccctgtc actctgcgtt cgttcgcttc ggttaccagc
     5881 atcgcaagaa gggtttgctg tttgcggaag acaaagaaag cagccgctgt ggcgagcagg
     5941 gtagtgaaga tgaccgtatg caggtgcgaa tccgagagcg agatgtcggg atagaggtag
     6001 tccatgatga actggtatac actcatcagg acgaaggcaa atattcccac ctttccaagg
     6061 gtgagataga ggttgacggg ttgaaactct ttatccatgc acgccccacg gggaagcggt
     6121 acggggtcct ctgcgtacct tgatctaacc gatttacggg gttttatgaa atgctgcacc
     6181 tgacaggtcc accggtccat agccccctcc ggtcgacgtt acctggtgta ccggggttag
     6241 aaccggagtg actgcggttc cccggtactc gagtgcattc tcccctgcaa tttgataaac
     6301 cttccttagt gggcaatata tcatacctcg aatcccggtt aaaaatgctg atactcctca
     6361 tcctggtctc ttttaagggc gtgcatgccc gcttcatccg ggaaggcgcc ctgctcgacg
     6421 gcgtcgtcct cctgcccctc tcggggatgg acgccggcac ctacatggcc cagatcggcc
     6481 ttcgctacct caccatcccg atcatcgcga tcgggatcgg atatgcggct gcacaggccg
     6541 tgcggggtgc gacggttcgg agtgcgactg ccgtcccggc aggagcatga gcaccgaagg
     6601 tgcgaagaac cagagcatga gaagaccgaa ggtcttcgag tgggggtaac ccctggaacc
     6661 ccgcccctca ggtgcggatc ttccggaaga ggtacgcccc gacgacggtc atgagcacgg
     6721 cacagccgac catgacggcg aggctgacga gcgggtggat ctgggcggtc ccggagaggc
     6781 cgtagcggat cccctcgacg ccgtaggtca gggggttggc aagcgtcagc ggccggagcc
     6841 aggcgggcag gttgtcgatg gggaagagcg ctcccgagag cccgaagatc gggaagatca
     6901 cgaagttcat gacgagctgg aagccgtgca tatcctccat cctcgacgca attgcaatgc
     6961 cgaatgcggt gaagagcaca ccgatcagcg ccatgaaccc gacggcgatc aggaaccctg
     7021 caatgccggt gggctggagc ccgatgaaga gcgccagcac catgaggatc agcccctgga
     7081 tgaccgccgt cgtcgctccg ccgagcgtct ggccgagcat gatctcgagg cgcgagaccg
     7141 gggcgaccag ggtctctttc aggaacccga actgtttgtc ccagatgatc tggatcccgg
     7201 agaagaccga ggtgaagagg acgctcatgg ccacgatgcc ggggatgatg aagttcatgt
     7261 acccctcttc cgtgccgggg atctggacga tcgagttcag cccgaagccg agcaccagca
     7321 tgaagaaaag gggcatcccg aggctgccga ggatcctgct cttcgaccgc aggtaacgct
     7381 tgacgttccg cagccagata gtatagacga tatccaccct tagcgcctcc ttgcccggtg
     7441 gaacatgcgc gtcgcctcgt tgccgctcgc ttcctcctcc cggatcgtcc tccccgtata
     7501 gtgcaggaag acgtcctcga gcgtcggctt atgcaccgag agcgaggtga tgccgatacc
     7561 cttctggtgg atggcggtca cgatcccggg gatgtgctgg tcggcatcct gcaggcggat
     7621 cgttaccagc ccgtcgtgac actcgatccc ggagatccac ggggcggcca ggaggccggc
     7681 aattgcgccg gggtcaactg aggctatggt gacgacgtcg ccgccgatcg aggctttcag
     7741 gttctctgcg gtgtcgagcg cgacgatcgt cccgcggtcg atgatcgcca cccgatcgca
     7801 gagccggtcg gcctcctcca tgtagtgggt ggtgaggatg atggtgatgc ccttctcttc
     7861 gtttaaccgc gcgatatact cccagagatg gtttcgcgtc tgcgggtcga ggccgagggt
     7921 gggctcatcc aggaagagca ctttcggctc atggagcagc ccccgggcga tctcgagccg
     7981 ccggcgcata ccgcccgagt aggtcttgac gaggtcgtct ttccggtctt cgagctcgac
     8041 gagggtcagg agttcgtcga tacgctgccg acgctcctcc ccgccgatcc ggtagaggcg
     8101 gccatggaag tccatgttct cgtatgccgt cagttcttcg tccaggctct ggtcctggaa
     8161 gacgatcccg atggatctcc ggacgttgtc ttcatcggtc acgatatcat ggccgttgac
     8221 cgtcgccgtt cccgaggtgg gtttctgcat cgtcgcgagc atcgagatgg tcgttgtctt
     8281 tcccgctccg ttcgggccga gcagcccgaa gatctcgccg ttctcgatgg taaacgagat
     8341 gccgtcgacg gcggtgaaac cgtcgaagga ctttgtcagg ttattgacgt gtatggccgg
     8401 cgtgggtccg gctccatggc ctggtgcatc cgtgtgcatt cgagcagctc cttgttcact
     8461 cctcgggcgg ctcttccggc gccttttcaa agattcggag catctcctct gcgatccggc
     8521 ggaatttctc cgccttgaca acggcgagcc catgctcctc gtcgccgagg accatcagtg
     8581 tgtcaccggg tttgatgtcg aagatctcgc gggcctcttt cgggatgacg atctggccgc
     8641 gtttgccgac cctggccgtc ccgaagatgt gtttcctgcc gtcatggtcg tgatggcagc
     8701 ggtggttgtg gaacatgatt ctggtcacct cgtcggatta ttcacacttt tcatactagt
     8761 atgatacatc atgccatatg aatctccccg gatgagtggg tgaaagcgtt tgcttcacgg
     8821 ggatccggcg gccttgaagg tgtcggtgtt ctttccggag gctaccggga ggctgattgc
     8881 tacccggcgt acaccggagg ggatcttttt cgaaggtccg gaagccggaa accaccggac
     8941 tcccgaggga cgctaaaaaa ggtattattt ctgccggcgc tctgcctttc tcgcggccag
     9001 gcgctcgatc gcggcgaggt cgacgctgcc gccggtcaca agttttccct gccggatcgc
     9061 ccggtcaacg aacccctttc cgatgtcgtt tcggataacg agtgtcgtgc agccgtcggg
     9121 cgaaccgaca gcccctgccg agacatccgc ttctaccgcc gtaaagtcgg tgcagacccg
     9181 gcatccgggc cggacagcct cctccagttc ggcgagcggg atggagaggt gccgctcgtc
     9241 ctgcaggtag acgtccagtt ttcccctgat gtccaggcgg cggatctccc agggctcgat
     9301 ccggcgctcg gactgcagtt tcccctcgac caattttgcg tagtcgaagg tctccgtgca
     9361 gaaaaggccg atcacgagcc ggatcgcctt cgcgtagggc tggaggagtt cgtgatcgct
     9421 cgcccggatc gcctgcgtcg ctcgtgccac acagggcacg cccacgacgg caatgtgccg
     9481 gtacttccgg gtgacgactg cctccttgag ggatgcgaga agcggcaccc accaggaata
     9541 gcggctcccg gcgtgctgga tgagttcttc cgaggaggtg atcaccgctg acgacggctt
     9601 catcgtccag gggtcggccg tcaccgtgac gaccgcatcg atcagcccct catcgagggc
     9661 gctcgcgagg atcgccgtca cagcaccgcc gctctgcttt cgctcaaccg ggaagagcga
     9721 ccgggccgca acgatctcgc ggtacggccc gagcatctcc tcctgcgccg cgaggtcgac
     9781 ccttgggcag gccgcgtagc atgccccgca gggtacgctg tcgttctcgg ccttacagta
     9841 gccgatgttc accggtgtat cggtgttccc cggcgcgaac cggagggcat ccgccgggca
     9901 gactgccacg cacgccccgc acccggaaca gaggtccgta gcccagacct cggactccag
     9961 gtccttgtat gtcttcatct ccatgcctat cactcccacg tatacttccc ggcaaagggc
    10021 cttgcgctcg caggaatgat ccgggtgtag tccgcgttga ccagcggttc cgcatcgatc
    10081 ccgaattctc ccgcaaattc ccggataacc ggggcaatcc ttctcctgtc ctcatccgtg
    10141 agcggcacct tctttgcccc gggagcgagg caggcgtcat ccacgtcccc acggatgact
    10201 atctctccgc cgtggatccc gctcccgatc tcgcgggcct tcatgacccc gtcgagacca
    10261 aggacgatca ggaggccgcc cgccatgtat tcaccaagga atgtctgagc ggatcccccg
    10321 acgaccagta tggggcgctc gccttcatac tgcttcatgt ggattccgcc ccggtagccg
    10381 atatctccgc ggacaaagac cttgccgccc cgcatgctgt gcgccaccgc gtcgccggcg
    10441 ctcccgtgga tcactacctt cccggcatcc attgtgttgc cgggcgcgtg ctccgcgttg
    10501 ccgtggacaa tgacggtcgg cccgctcatg aacatggcga gatcgccgcc cgcgacaccg
    10561 tggacctgga tggtggcgtc tccccggaga ccgtcgccga tgaaccgctg tccgaggaca
    10621 ttgttgatga tgatctcgtc ggcaccgtcc tccacggcct tccggatctg ctggtttaag
    10681 ggagtgtagt gcactccctt cgcatcgatc gtgacctgtt ttgtcattgt ttgctcacgc
    10741 tcccaccggc ctggtgtcgg gaagatcagg gaccctcgtc cgacatagaa gcccggaatc
    10801 ggtcgaggtt gccctgcaga ttatcgaggc tatctatccc ggcgtctcca gggcccgtgc
    10861 cctcacggat gctgccgcgc tcgttcatcg tgaagagggc ctgccggatg ccggatcgtt
    10921 caccctccgg ggcatcctgt ccgctgcacc tgatctgcca cacatggtag ccccggcggt
    10981 atcttctgct gccgtttact ataattctgc ccgggtatat ttatgtcagt aggtatttat
    11041 gccatcgggt agaagttgac ttccacatgc cccgtgatgc cacttctgcg atgcttgagc
    11101 gcgtcgaaca ggacaacgtc cgattccttc gcctgcagtt taccgacctt ctcggcatgc
    11161 ccaagaacgt cgccatcccg gtgaagcagg ctgaaaaagc cctgaccgat ggtatcgggt
    11221 ttgacgggtc gtcgattgaa gggttcgtcc ggatcgagga gtccgacatg gtgctcagac
    11281 ccgacctctc cacctacact ctcctgccct ggcggtcggg gaagtatgcg gaggcacgtt
    11341 tcgtatgcga cgtctacaag gccagcagcg gcaaaccctt cgagggcgac ccgcgctacg
//
LOCUS       DAJB01000073            4775 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_188188, whole genome shotgun sequence.
ACCESSION   DAJB01000073 DAJB01000000
VERSION     DAJB01000073.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_188188"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          930 a         1346 c         1492 g         1007 t
ORIGIN      
        1 gtgaaggtcg ggtatggccc ccgggaggca acccagagcc ccggcgatat ccgagaggta
       61 aagggtatgt gccctcatgc gaagagaagg tcccgggtga cctgcccgcg gatctccggg
      121 cggagtgctg cttccggcac gacatccacg ccggaaccga gtttttcctc gaggtactgc
      181 gacagcccga caaagtcaaa gagatccgca ccgggaccga actcgacaag tacgtcgata
      241 tcgctgccct ccccctgttc ctcacgggca tacgacccga agagggcgat acgctcgaca
      301 tgataccgtt ccctgagttc gtctttcaga gaacggagga tgtcgagaac ttcggtacgg
      361 gtcttcatat gcggcacgtc cctgctatac gtctcccttc gtcgccggat tgctttaatc
      421 tattgttcgc cgtgagccag gttcagtcac tggcaccgcc acggttcgtt gccgttcctg
      481 ccccgcagat aggttgttcc tgctctggaa acattttgtt tccaatcagg aaacactacg
      541 ggccagggag aagtcctgtc agatccccca caaccgttct ataggctaaa cgatcgttta
      601 ccctattgta tgttcccggt tctagacgtt cgtcgagtta cgcgtcctcg cccgggtctt
      661 tccctattgg tgggaacaaa gaggaagacg acccgtttgt tgccagtgtt ctccggaatc
      721 acaccctcgt cagggggctg cactatgagg tggagggagt gcatctgccg gagcctcatc
      781 cgccctgatc ccgaggccgg aggagcacgg agagggccag gatgaacgcc agtgcctctg
      841 cagcctgcca taacgagatg gctactctgg agagggtcat gtgttgttgc cggattctac
      901 cgtcgcaggt gtagagaaga ctggaccggt ataaaacata ccgcatgctc cgggcatgaa
      961 cccatatccg ggtgaaagat atctcgaact gtgcatgtcg gccagcgatt ccgggttcat
     1021 ctggatgccc ttcataccgg taaccttccg gcagagcaga gtttatcgta ccggggtgga
     1081 gcagatacat tattatagtg ctatcaaacc ctttgcacgg tgttgttatg gctaacgtaa
     1141 acttcggtgc cggctcccct gagaccggcg ctgctatggc cccgtcacgc tggctcgtgc
     1201 tgctgctcgg catcatcgcg gtcctctttg gcgctctctt ctttgtctac cccgtccaga
     1261 cgttgactct ccttgtcacc gtcctcggca tttactggct gttcaacggt atcgtcgtgt
     1321 tcatcagcct ctttacggat aaaaccggcc ggggctggaa actgctggtc ggtatcctcg
     1381 gcatcctcgc aggagtcctc gtgctcacct accccctcta cagtgccatc ctcatcccga
     1441 cggtcttcgc catcatcatc ggcgtggagg ggctcatcat cggggcggtc tacatggtgc
     1501 aggggttctc cggtgccggg tggggtacgg ggattctcgg tatcctgagc atcatcttcg
     1561 gcctgatcct gatcgcaaac ccgctcgtcg ccgccgtcgg cctggtgctg ctccttgcgg
     1621 gacttgccat catcggcgga atcgcagcca tcgtcgtgtc gttccggctg ccggggtgag
     1681 ggagcgatcc ggagaggagg gatccgtgct ccgggtgtga ggcacggcct ccccttctct
     1741 ttgctcgtaa taatgcgtat tttgccactg ttttctccgg gatgagggga tatgagagcg
     1801 tcgttgtgta acactctgtt cgccgcaata catgggggta attatatata attcccgtta
     1861 tcatccggct ccgggcagtc attctgctcg ggagatgggt atgaagaaaa cgggagtact
     1921 gttcatcgcg ctggtcctcg tcctggcctg tatcggtgcc ggatgcgtcc agccgtcgga
     1981 ggaggaggcg gaggcgcagc tctgccagga tcttggagaa ctcggggcag cgcttgagag
     2041 tatggagaac accagtctga ggacttctgt gggagatatc cgggagggac gggaccaggt
     2101 gcagtccgcc atggagggtg tcagggagtc cgcgggccag ctcgccaacg tccgggtcga
     2161 cgagctcaac gcggcgtatg aagacctcga ccggtccgtg gaggaccttc ccgacgatgc
     2221 gaccgtcatt gaaggcatcc agaccatccg cccgcaaata caggccgtcc gggccgagca
     2281 gcagaacctt tacacagacc tgaactgtac ggggcagtga gcgccgcgag gattggattg
     2341 cagcagggca cgtgaggaga gacctggcgg gtatcggaag gggcggtcga tccccatacc
     2401 cgccggacgc acatgtcatg gccgcatctt tctcatgcga agctcacgtt attgccccgt
     2461 agcggcatcc aaccggatac cgttgcggga acatggagga acgatacatg cgtaaagaag
     2521 atccacggac gttctggatc ggagtgatcg tcggactcat tgtcgcgctc gtcattacgt
     2581 acctgatagc gatcggcggt gcgtttgtcg gaggtattgt tgcgggctgg attgccgggg
     2641 gaggaaggaa tacccgggag atcggaggaa gagccggtgt gtatacgggc cttctggatg
     2701 cacttgccct ggcagcggtc attgcagtgc tgggcatcga ggccgccccg ggcgatctcg
     2761 cccttcttcg cttcctcgga tcgacgctgc tcatcacgct gatgctcttc ccggttctgg
     2821 gcttcgtcgg ctatctggga ggggtgctgg gaggagccct gaaggagcgt tgaaccccac
     2881 ttgtggatat gaagggattc cattcgattg acgtgaaatg cgtcatagac cgttcacagg
     2941 agacgggtcc cacggcaatg gtgtggagag gacttcccgt atttatgccg gcgtgaaggt
     3001 gaggaatgat ggccgcccgc gatgagagtc tgaagacgtc cggtcgagat cgcctgaccg
     3061 acattcttcc taccgaccgg ctgagtgcgt ttgccgacgg agtcttcgcg atcgtcatca
     3121 ccctgctcgt cctcgaactt cccgtgccgg aagccaccga agacctgctg tgggcgcttc
     3181 tcgaagcgtg gcccgacttc ctcgcctaca tcatcagttt cgcatttatc ggcgggttct
     3241 ggatcacgca cacatccatc acccggatga cagcacacga agacggcgtt acagtccgca
     3301 tggcgctcgt gatgctcttc ttcgtctctc tgcttccgtt ctccacgagc ctgatggcca
     3361 cgcacctcat cgggccaggc tcccgtctgg cggtgtttat ctacgggctg gacctgttcc
     3421 tcgcgtcgct catgctgaat cggatcatgg actcccttat ccggcgtccg gacctcctta
     3481 tcgacggcct tgcagacgcg gacttccggg gcatggagca tcggcggcga tacgggatcg
     3541 tgctcgatgg aatcggcgtg ctgctcgcac tgttcctgcc ccgggtggca atcgcaatat
     3601 acatctccgc ggcgatctac ttcattctcc aacctctgtt cttcctgaga acccccatgc
     3661 ggtctttgga aaaggaaggg cggtgagggc actgcccaca ggggaaacgc cgccctgcca
     3721 ggaggtgttc gtgcacaatt cgcacctgta ccggagggag agcaggaggg tttcccggcg
     3781 cgggaagcag cgccgcagca aaaacgatgc ggtcgtctgc tgtgacatca agagcacgct
     3841 gtagatcgac tggttctttc ctgttcgttc ggatgggatc tgccggtccc ggcacccgga
     3901 accgcgagaa agcatttctg caaaattata ataatcttgt aatattattg gtgcaggcag
     3961 ccgtttgccc cggtgactct cgtcatccga tcaccgggac cgtgtgattt cgctggagag
     4021 ccatacaggc atccggaata cgggtggggt gacggcagcc tagatattca agggtgatac
     4081 ccatgatgcg aagaagagcg gaaggcggcc cgcgggggcg cgaagaggcc ggcggcacac
     4141 atcggtacaa gatgaaagag cggctcatcg atatcgggga tgactactga atcgaggacg
     4201 cagcgggaga acgggcgttc aaggtggacg gaaaagcgct ccgcgtgcga aacaccctga
     4261 tcatccagag caaggagggg caggacctct ataagatcca ggaaaggatg ctccgtatca
     4321 aggataccat ggagatcgag aagggcgcag gcggtacggc ggcgaccatc aagaaggcgc
     4381 tgatcgcgcc gctccgtgac cggtggacgg taaccgtccc ggggcaggag gactgggagg
     4441 tgcaggggaa tatcctcgac cacgagtacc atatcgaggc cgggcggaag cggatcgcgg
     4501 aggcctccaa aaaatggttc cggatccggg acacctacgg cgtggaggtg gaaccgggat
     4561 acgatgacgc gctcgtcctc gccgttacgg cggcgatcga ccagatggcg cattaggccg
     4621 ctattggaga agaaaagggc gaggggcaat caacaggccc gtcgcggcag cagggcaggg
     4681 tgcccggttg ggggaggaac ggctcttata ccggagggtg tctggatatt tttgctcacc
     4741 tcgtcgggca aggagacccc cggtttcaac cgggg
//
LOCUS       DAJB01000074           14753 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_90271, whole genome shotgun sequence.
ACCESSION   DAJB01000074 DAJB01000000
VERSION     DAJB01000074.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14753)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14753)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14753
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_90271"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2673 a         4614 c         4566 g         2900 t
ORIGIN      
        1 tacgggaagg agatgtcctg gtcgttgtcc cctggagttt ccaggacgag aagtgcgaca
       61 tcctctaccg ctacacgaga ccccaggtgg agtggctccg gaagaaccgg tatctctgat
      121 atctttttta taacatattt tcctgtacgg caggggcaaa ggcagcagaa ttgagttgcc
      181 gggttcaaaa aaggttagat atcgtgaatc ttgatctcgt actcttttat gacaagtttg
      241 ttgacattat cccgcttata ttcgttggga gtgggcgtat acatcccgac cgaactgctg
      301 gtcaggtagt tctcccatgc caccgagtag tcacctgacg aaccgctcgt atactccacc
      361 gtcactttat ctacggacca aaccggtatc gcctgcgtgt gcgcctcctc caggctcatc
      421 ctcaccgttg ccatccccga cttcgccatc aggtcgtcgg tggtgatgtt catgacgtag
      481 atgacgagcg tattttcacc cgtcaccgga ttaacgtcat agaaccaccg gggctcggca
      541 agcatcgccg aacccgtcga cccgtcctgg cgtgtcatca ccgcgccgtt ctcgagggtg
      601 atcacctcgt tgctgcggtc ggaacggtag atcagtgcac ctacggtata gttctcctga
      661 aacagggtgg tgccgttcgt gacgttgatg aagccgccgt actgagtgga gttgaccacc
      721 tcgagcgtcc ccccgctcac ctggagcgac gtctccttgt agggcacgtt cttgtagcag
      781 aggctcttca tatcattctg gatgacgatc atcgcccgct ccatgttctt cacgtcggtg
      841 ttgctctgct ccttgaggag caccgggtag ccgtagagcg tgaccagact gatgccggtg
      901 atgacgatcc cgaggatcag gacaaacccg atcgcctcgg agaccgcccg ttcattcatc
      961 ttcacggatc tctccctccc taaaatccac ttgagtcgta ctggatcatg ttccaccccg
     1021 cgccggtggt gttgccggag acacctttcg tcgccccgat accggcgatg gcgaccgtgc
     1081 tctggatatt acccccctgc acctggatag cctggcttgc ccggttctcg ccctccatcc
     1141 atacgtccac gaagtagccc ctcccggcaa catcgtcggg gatatcgaac ttcgtggcga
     1201 tggtcccgtt gccgggcgcg atcacgtaga ggtccacgat ccgggtgctg acaccgttcc
     1261 cgatgtccac gaaggcgtgg tagcggagtt tatccgccgg cccctccatg aagacggcgt
     1321 tgatggtgaa catcatgacg atcatcagca cgagcagcac gccgctgatg atgacatact
     1381 ccatcaatcg ggtgacgccc tcctcgttca ccggttcagt aatacacggt ctcatcgtac
     1441 tccgtcaccc cgttgttgta gtggatgtct accgggcgga gcaggcgccc ggagatgtcc
     1501 tgcggagcgc ccaccgcgaa gccgaccacg gcgttctttc gttcgagcga tatcctgacg
     1561 atatcctcct ccacatcgtc cagatgtcca tgagaatcaa tttcctcgta gcggtcataa
     1621 tagtcggaga tctccgatcg caggtcccgg atatcgttct tcgggaactc gagcaccccc
     1681 tccgcggtcg tctgcccgac gagcgccgac tggttgatga tcagcgcgag gaagaacagt
     1741 cccacggcga cgagaaggcc catcagcacg atccactggc cgtcttcgtt catgcacgcc
     1801 atagcagcac ctccacgaga acggcgtgcg gccgcgaaat ctccatgttg ggtataccgt
     1861 agtttcctga tggcagttgc acccatcgcg tcacccgcac ggcgttctca cgcccggtga
     1921 actccgggtc aggtgaggag agcagggttg tgtttacgct gccgcccgat gttctatagg
     1981 agaggtagac ctgggcccgg aggttatcat ccgggtctcc ggatcgcatc ttacagccgg
     2041 cgaggaactt gtcccggagg tcattaccgc ccatggttcc tgtttcattc ttgacaaacc
     2101 cgaccagctc gctcgtactt ccattggtat ccggtgtatc catcatcgcg agcacgtcgc
     2161 tcccgagctg ctcaagctgc atatccggga tgtgtgtatc ccccggggtg tagatgctcg
     2221 tcgtgctgat gacgatgtac gcggtgagga gcatgatgac cccggccgcg acgccctcca
     2281 ttgtgtagag ctgtcctgca tcgttcacca taccgccacc tccaggacgc cggtctgaag
     2341 gtcggggatg gtgacgttat cgtagtcata caggaacgtg ccgttcatga aggtcctctg
     2401 cggttcaccg tcatcgaagg tgaatcggat atccagaatg ctgttctggt ccagggtgaa
     2461 ctgcgaggtc gcggccggtt tcagcaccag ggagatgttg gattcgaccg ggactcccgg
     2521 actgagcgag tgtagggtcg gagtgccatc aatatgcagg gtgtagaggt ttgagtcctt
     2581 cgtactgtac gagaacggga tcttcgtgaa ctgcgggatc ggcctcgttg gatccttctt
     2641 ccagaacgtc acggtccgga gcgttgcgtt cgtggacggt tcagtcctgt tcaggtaatc
     2701 accaaaatcc gtgatcgtca cgttcaccgg ctcggtcctc gggtcgatcc ggtaggccgc
     2761 tccgacggat gggtcgagca atttgctgaa gttgagccgg gcgatgaact cctggttcga
     2821 ggagagtgac gtgtactgcg gcgcgaaagt tgttccgtta atcaccgcca ctcccggctc
     2881 cttgatcttc accacccggc ggatatagcc gtagccagga gggagagggt cgccgacctg
     2941 cttgagatcg taactcccat ccgggtgtga ggtgttgagc gagaaattgt aggagtaggg
     3001 aatgtcccca aagatcacct tgctgcggta atcgtccggg gtaaaaaacg agtcgttgaa
     3061 gaacgcatcg accttcgtcg agaggaggat gttcggggtc tccttcgata ccgcgaggcc
     3121 catccgctgg atctcgtcct tatggacttc gtcgttcatc tcccagggcg ggtagaccgg
     3181 ccagccgggg tcctcgacaa ggatcacccc cgtccggtag gcgaccgcgt cgtagtcgat
     3241 gccgctgctc tcgaggcccg cgaggagccc cggcaccatg ctcaccacca tgatcagggc
     3301 gagcatgaag atcgtgaacc ccacgagaaa gtccagcgac atcgccccgt catcggccgc
     3361 cggtcttata cgatcacctc cccggtatcc gcccggaacg cgagggtctc catccccgcg
     3421 tccgatcgga tcgtgatcgc atagaccccc tccgagagag cgatatcaac cgtatcggca
     3481 ccgagctgct ccattgcggg tgagagcacc ccgtcctgca tcgcaaggag atcctccggg
     3541 gagaggacac tggtgaggtt cacctcctct gcgggcaggg gggcacggag cgagatctcc
     3601 ttccagcccg tgagaccgaa ggtcagccag cgcacctcct cgccatccgc aagaccgagg
     3661 gcccaggagg tggcccgacc gtcgaggctc atgccggtcc cgagcacctg gtggaccgat
     3721 gtgccggtga tgttcaggcc gccttcggcc ccgagcaggt ccagttccgc gagagccccg
     3781 gtgagggtgc aggcccccgg cggctccggc aggtcgatgg gggtgatgcc cccgcctgac
     3841 ggcgacgagg atgcaccgag acaaccgcac gtggtgcaga ggatacatac tgcggcaagc
     3901 agggtcagga cgcccccgga gactctcatg taaagcagtc attatacgtg agggcatata
     3961 aataaccccc tctcttgtat attcaacaat ctgccgttag aatcggatat tggaaacgtg
     4021 caggcaaccg gtggcaaaaa tatttcgtcc ggtgtgcaga cccgcaggtc ggaacggttc
     4081 ggcccttata tgcagaggga gtgggcccgc gagctccggc gggctatcgt aacatatatc
     4141 catctgttcg taacaacatc tcatgcaggg taagcacatg tgtaatacca taccccccgg
     4201 caagccggga gaagcacacg accggttcgc agaagcggcc gccttccacg gccacgtctg
     4261 tcccggcctc gcgacgggct accgggcggc agagatcgcc ctttcaaggc tccgctccgg
     4321 ccgttcggaa gacgaggaac tcgtcaccat cgccgagacc gatgcctgcg gcgtcgatgc
     4381 catccaggtc ctgaccgggt gcaccgccgg aaaaggaaat ctcctcttca aggactacgg
     4441 caaacacgcc ttcacgttca tcaaccgccg gaccggggcc gcgatccgcg tggcggcaaa
     4501 cccgtcgttc gacgtcgact cactcgaccc ctgcctcgcg ccgctccgtg cgcgggtgat
     4561 gcagggaaag gcctccgatg aggagcaggc cgaattccac gagcggatgc atcggatcgt
     4621 cgatgtcatt ctggagatgc ccgctgaaga actcttcacc atccgggagg tcgatcccgg
     4681 gatcccggag caggcacgga tcttttcgtc ggtgccgtgc tcgaagtgcg gcgaaatgac
     4741 cgccgagtcc cgggtccgcc tcgaggacgg gaagttcgtc tgttacgcct gcggaaagga
     4801 gtactcccgg cgcgggtgag gggtttcctg cctcaccgca ctattttcgc tatcgggatc
     4861 tcaaggatat cttcggcacc cgcggccttg agcggcggaa taaggccgtt gacgagtccc
     4921 ttctgcacca ccgtctggat gctgaagtag tctatgccgt gcagcctgcc gatggtgggc
     4981 tgtttcatcg caggaaggac ctcgatgacg cggtcgagcg ccgccgccgg cacgttcatc
     5041 gtcaggagga cctggtggcg tgcctcgatc accccgagga ggagggtcgt gatctcctcg
     5101 atctcccggc gcttctccgg gtccagaagg gactcgcggt tcgcgaggag cgcggtgtgc
     5161 gactccataa tctggccgac aatcttcaac ccgttcttct tgagcgtgct cccggtctcg
     5221 gtgaggtcga cgacgacgtc catgaggtcg gggaccttcg cctccgaggc cccgtaagag
     5281 gcaaagagcc tgacggggac ctcgatcccc ttaaagaact gctccgtgat ccggggatac
     5341 tcggtcgtca cgcggctgcc cgggcggatg gcggtgacgc tgtcgattgg ctcgtcacgg
     5401 tgaaccgcga ccacgatctt cacgttccct tccccggtct tgctgtagga gaggttggcg
     5461 acctccacaa cgtcggcgcc gctctcctgc acccagtcaa gccctgatat cccgagatcg
     5521 aagtagccca tctgaacgta gagcgggatc tcctgtggcc ggaggatctt caccttcccg
     5581 atccttgaat cgttgatgcg agggttgtag tcgcggtcgg tccgcctgac ctcgaggtcc
     5641 gcctccttga agagctggag cgtctgcgct tcgaggcttc ccttggggag agcaatcgtt
     5701 atcatgaaga ggtgatcggg cagaaaaaga attaaagatg gtgatatgga tcttcaatcg
     5761 atggtgtgga cgacaagccc tgagagaagt ttcggctcga accaggtgga cttcgggggc
     5821 atgacaccgt ctttatcggc aatcgcgagc accgtctcga cccgcaccgg ctgcatcgct
     5881 accgcgaggg cgtatttgcc ggagtccacg agccgctcga gttcggagag cggctttgcc
     5941 ccgcccatgt actggagcct cggatcaccc cgggggtcgg tgatgccgag catcccttcg
     6001 aggacatcct tctgcagaac cgagacgtcc agggacccga tcaggtcggt gaggtccgcg
     6061 accggccggg aaacctcgta ccaggttccc tccaggtaga agtgcatgac atgcaccaat
     6121 cccgctccgg ccgcgagcgg tgggacctgg tagcccgcag catcgacctt cccgtagggg
     6181 cggacattcc agcccgattt cgggagcgcc tccatgaact cgcggggagt ggtgtagccg
     6241 ccgaggtccg tgacgagccg gctgtagccg tggatgcgga cccggtcgtg ggcgaagagg
     6301 accaccatga acttccctgc ctcttcggtg aacctgccat cgctctgccg cctctccgcc
     6361 acgttcaccg ccgacttggc gcggtggtgt ccgtctgcga tgtatgtgtt cggaaccgcc
     6421 gcaaaaagcc cctccaggcg cgagaggatc gcttcatcgg tgacccgata gacctggtgg
     6481 agcacgcccg atccggtcgt ggtgctcccg tcaggcgccg ctgtgccgat gagggagtgg
     6541 acataggaga agatcaggcc ggggtcgcgg tagagcagga agacgagacc ggtgttggcg
     6601 ccgaccgcgt cgatgtgccg ggtccggtcc tcctccttgt cataccgggt gagttcatgc
     6661 cgccggatct gccggctctc gtagtcctcc gtcgccacgc aacagaccag accgatgaac
     6721 tcctcgccgt cctggacggc ccggtagacg tacatcccgg gctccggatc ctgctgcatc
     6781 aggccgtcgg cgaccatgcc gtcgaatatc tcccgcgccc gctggtagac ccggtcatcg
     6841 ttcggggcga ggtcggggag ttcggcgtcg ggccggctta cccgcaggaa actcaaggga
     6901 ttcttctcga tgcactcccg ggcttcttct gccgtcacca catcgtaggg cacggaagga
     6961 attttttcgg agtaccgccg ccccggacgg accgccgcga aacgatagat ctgaaccata
     7021 aatgccgcca ctcgtatgtg cctactattt tgtcccctga cactatatta ggtaagacac
     7081 tccacgaggg agagaccggt aactatgatg ccgcttcaac tcaccatccg cagggcacag
     7141 cctgtagaca tcccgcagat agttgccatc gaaaggacag cgttcgtcga cccctgggac
     7201 gagaggatgc tccaggaatc gctcgcctat tacccggaga cgttcttcgt agccaaaaac
     7261 aacgaaggcc tggccgggtt cgtcgcaggg gggattgagg ataccgggga agaggtttac
     7321 gggcatatca tgaacctcgc cgtcgccccg ggataccggc ggcggggggt cggacaacag
     7381 ctcattcgcc gcctggaacg ggagtatgca gttttaggcg cgagcggggt ccagctggag
     7441 gtccggatca ccaatgccgg ggcgcaggag ttctaccacc gtctcggcta ccgggaagtc
     7501 ttccagattg ccagttatta cgcaaacgag gaagacgcct tcgttatgat gaaatggttc
     7561 cggttctagg tgcgacgggc cgaagggccg gagcaggagc accgtcaggt gctcgaactc
     7621 cctccttgtt acccccgacc gtgctcctgg tgggcccggg ccaggtgctg ggccgcgagg
     7681 gcgccgagga gcgagagctc gccggcgagc acccccgccc ccacgatctc cgcgaacgcc
     7741 ttcgcatggg ctcccggcgg gtcgccgcca cccgcgacgc cgagcagttc caggcattcc
     7801 cgctgggtat cgacccccgt gccgccgccg accgtcccca ccgggagcga cgggagcgtg
     7861 accgagacgt agacgccgcc gtccacccgg tcgacggtgg tgatcgcggt gctcccctcc
     7921 acgacgtggg cggggtcctg cccgcaggcg atgaacatag cggcgacgac gtttgctgcc
     7981 tgggcgttga acccgaacga ggccgcccgc gcagagccta ccaggttctt ccggtagttg
     8041 acctcggcga gggtctccgc atcggtcttt aagaggtcag cgaccatcgc gtcggtgagg
     8101 cgcacccccg cgaccacggt cttgccccgg ccccggacca ggttgatcgc cgccggcttc
     8161 ttgtcggtgc acatgttccc ggaggtggcg acgaggcggg ccccggtctc ccgctcgatg
     8221 agctcgccca ccttctggcc tgcgattgtc accatgttca tgcccatggc gtcttttgta
     8281 tcgaactcga gccggacgaa gacgttcgtc ccggcaacgt aggtgacggc gtcgaggaac
     8341 tccccgtgct tcgtggtgct ctgtgcggtc tcgcggatct cggcggtgtg cccttccacc
     8401 caggcgacca cctcccgggc atggacgacg tcgcgggcgg caaagaccgg tgcccgggtc
     8461 atcccgtccc gcatgatccg cacgtccgca ccccctgccc gggtgatcag cgagcacccg
     8521 cggttcaccg aggccacgag cgccccttcg gtcgtcgcca gggggaggta atacgacccg
     8581 gcggcatact cccccttcac ccgtagcggt cctgcgacac cgagcggcac ctgcacggca
     8641 ccgatcatgt tctcgatgtt gcgcttcacg acccggtcga tcccgatgga gaacgacccc
     8701 accgcaggaa gggcaacgtt ggtctcaccc tcgatgaatg cccgccgcac ccggacggcc
     8761 tcctcgggca ggagttcctt ttcgagcgcg tagagtttga gggagccgtc tttcagtctc
     8821 ccgatatact catccatgaa taagtatgag aacgcatacc taaagatggc tggtggtccg
     8881 tatacgaaag gaacagtggt gccttgcggt gccgaagcga gaggccgagc agacacggcg
     8941 ccggctgctt gaagaggggc tcctcgacca gggcttaaag ccgcgccccg agggggacgc
     9001 catcctcttc ccggtgacgg aggagatcgc gggcgccgcc tggtgcgagt tcgaggccgt
     9061 tccggaacgt gccgaactcc cccgccacga gctcgtgggc gggatcgcca tcatgcagga
     9121 cgacgacccg gaggccgccc ggatgctgct ctcctcccgg ccgtccctgc agacggtgct
     9181 cttcccggca ggtccggtcg agggtgagta ccgcacccgc cggttcgccg tccttgcagg
     9241 ggttcccacc acccgcaccc gggtgaccga gtacgggctc tacttcgacg tggaccttgc
     9301 cctcgcctac ttctcggccc ggctctcgac cgagcggcag cgggtcttcg aggcgatgga
     9361 ggagggggag cgggtgctcg atatgtttgc cggcgtcggg ccgttcgcca tctccctcgc
     9421 ccgcagggcc gccctcgtcg tcgccgcgga tctcaacccc gcagcggtcc accttctggt
     9481 ccacaacatc gcgctcaacc gggcgaaaaa catcattcct atgctcgcgg acgccggaaa
     9541 cctcaccaga ctcggctttc accccttcga ccgggtcgtc atgaaccttc ctctggccgc
     9601 accgcagttc ctcccggcgg ccgccgcgct ctgccgggac ggcgggacga tccatctata
     9661 cgccctccag gcgcaggagg gtgaatgtct ccccctgatc cgggctgtta cctccgggga
     9721 agtcaccgag cggcaggtca ggacctactc gccgggcagg tggcatgcgg tatacgatat
     9781 cgtcgtggag aaagagggct gctaattttc tccggcccgt aggggtgtgg agtgctatgg
     9841 cttcacgcga aggggagtta ccgagaacca ccgcaccgac cttgcgtgat tcgacaatgg
     9901 aggatagtat ggggttcctt tcgcgtgggg cccccaaaaa aagtgaaggg tcactccgtc
     9961 ggcacgaact tcgtgccgta gtggatgacg ccgctctcgc cgtccgccgc tatccggcgg
    10021 aagacgctgt gcacccgcat cccgatcttc gcctcttcgg ggaggcaggc gatctggctg
    10081 gtgagcctcg ggccctcgtc gagcgctatg accgcgagga cgtacggggt ggtgttctcg
    10141 aactggtcgc ttgccgaccg gatgaccgtg taggtgacga ccgttccctc accgttgaac
    10201 ttgtggtcga cgatcttgcc gctcctccgg cagtcgggac agagcgaccg gggcgggaag
    10261 aagtaccgcc cgcaggtcgt gcattgcgtc ccgatgaggt tgtagcgctg cgggatcttt
    10321 ctccagaacc gtgaaaccga catttttata cccccaggat gtgcacgacg accgtcgcgc
    10381 cggtgccgcc cacgttatgg gccataccga tctccgcgtc cacctgccgg gccccggcat
    10441 cgccgcggag ctggttgacg atctcgtaga cctgcttgat tccggtcgca ccgaccgggt
    10501 ggccgcaggc tttcaggccg ccgctcgtgt tcacggggat ctttccgccg agggccgtga
    10561 ccccttcctc ggtgagcctg cccgcttcgc ctttcttgca gaatccgagg tcttcgatcg
    10621 cacagatctc ggcgatggtg aagcagtcgt ggacctccag catgtcgata tccttgtggg
    10681 tgaggcctgc ctgcgaaaac gcccgcttcc ctgccgccac ggaggcgtcc agggtggata
    10741 tgtcccgccg gtcgtggagc gcgatggtat cgcttgcctg ggcgcttgca agcacccgca
    10801 ccggcgagtc ggtgaactca cgggcacgtt cgagcggcac caccacgacc gccgccgcac
    10861 cgtcggtgat cggcgagcag tcgaagagcc gcagagggtc ggcgaccagc gaagagttca
    10921 gcaccgtgtc gatcgtgata cggttttgga actgggcgat cgggtttctt gcaccgttct
    10981 cgtggttctt cactgcaacc tgtgcaagct gctcccgggt gaggggatag cggtgcatgt
    11041 agtcggttgc gatcatggcg tagagccccg ggaaggtcgc cccggcaaac ccttcccact
    11101 cgcggtccgc ggcgcttgcg agcatgtcca cgctcgcccc ggtcccgaca tcggtcatct
    11161 tctcgacgcc cgcggcgacg acgatatcct ccatgccgct tgcgaccgcg ataaccgcct
    11221 gccggaaggc aagcccgccg gaggcgcagg ccgcctccac acgggtcgag gcgacgtggt
    11281 tggtcgcgag ccctgcgtaa tccgcaatca gggcaccgat gtgctcttgc tcgacgaacc
    11341 tgcctgcgct catgtttccc acgtagaggg cgtcgaggtt ctcgccggtg accccggcat
    11401 cctcgagcgc cagcgcccct gccttgacga agaggtcacg gaacgaggtg ccccagtgct
    11461 ccccgaactc cgtgcaccct actccgatta ctgctacgtc tctcatttct gcatcacgat
    11521 ctttcccttg tgtttggcat acagtgcata gtcgaggtaa gttgggtttg cgagaagctt
    11581 cgcgacactc ggtgccgcag acctgttgaa atcctctgat tcgatggcgt tggtgaccgt
    11641 gatatcgaag gcgtcgctcc ctgctcctga cccgaacgag gtgacgaaga tccggtcgcc
    11701 cggttttgcc acgtcaagcg tggccgcaag cccgatcatc gatgctcccg agtaggtgtt
    11761 gccgagcctc ggaacaacga ggccgggctc gatctgctca gccgtgaacc cgagcatctt
    11821 tgccacccgc tgcgggaact ttgcgttggg ctggtggaag acggcgtagt cgtagtcctt
    11881 cgggctcgtc cccactgcgt cgagcatcag ccgggcggcg ccctgaacgt gcttgaagta
    11941 acccgggtcg ccggtgaacc gcccgccgtg gcgggggtag ttctgtcctt cacgccgcca
    12001 gaagtcgggc gtgtcggtgg tgtaggagca ggtgtggttg atatcggcga tggtcttccc
    12061 ttcgccgatg acgaacgcag ccccgccggc cgccgccgtg tactcaagcg cgtcgccggg
    12121 agcgccctgc gccacgtcgg caccgatggc gatcccgaac gggatcatcc cgctcttgac
    12181 catgcccatg caggtctgga tgcccgccgt tccggccttg cacgcaaact cgtagtcggc
    12241 agcggtcatg ttgggggtcg ccccgatcgc ctcgccgacc gtgcaggcgg tgggcttgac
    12301 cgcgtaggga tgagactcgc ttcccacgta gacggcgccg attgcctcgg ggtcgacggt
    12361 cctccgccgg agggcggcac gggccgcctc gacggcgatc gttgcggtat cctcatcgag
    12421 atcggggacg gacttctcaa agaccccgag accccgggtg atgtcgtctg cgttggcgcc
    12481 ccatacccgg gcgatctccg ggacttttat ccggtatcgg gggatgtaga cgccgtatga
    12541 ataaatgcct accatagttt tcctcgtagt gaactgatga tcgtctcgac gtccatctgc
    12601 gtacagagta ccgttatgcg gtcgcggtcc gcgaggcccg ggacaatcgg gtgcacctgc
    12661 tccgggctga tgccttgcag caccacgcag cggggcttaa atggcgtgac ccggattgcc
    12721 accatggggg acttcccggt tgagacctcg gcgaagatga gggcacgctc cgtgctccag
    12781 ccgtagatgc ggttgaactc gctcgaagag agctggagaa tcgcattgag gctgttgatc
    12841 accgtatacc cgtagacggt ctggtccatc gggccgcaga gtgttatggc gccgataact
    12901 ccagcaaagt cggagaggct cacgggggat gcatattcat ggatatcgta gatgacgtct
    12961 tcgtcgaact cactgtacag aatcttagca aatttgttga tgaaccggcc gccgttgtcc
    13021 tcgtcgatcg agaggatcgt atcgacaatc ttgccgacga tggcggtccc cggactcttt
    13081 cgtcgtccgc tttcgtaatc actgataaca gatggagaga cctcgagacg ctcggcgagc
    13141 actccctggg ggatgctgaa actctgccgc cacttcttga gggcctgtcc tggtgagtcc
    13201 gagagtgtga tctctcctgc cattttctcg gcaagctgat gccgcactcc ggatttcata
    13261 gagctaatgt taccacccgg acataaataa ttagcgtatg ccacatcgac atttcacgaa
    13321 ggtcccctct gggggatcgt tgcgcaaccc gtatatagta tgcatctcaa ttctgatatg
    13381 atggttgaag cggaagacct gcagtccttg aagatggtcg ccctgctcgg cgggtgccgg
    13441 ggcccgatcc ggctctcatc gcagtcactc gggaacgaac tgggcatcag cccccagacc
    13501 gcctcacggc ggctgatcgc gcttgagcgg cagcagttcg tcatccggtc gatgaagccc
    13561 gacggccagt atgtcgccgt cacccgggag ggcgagcagg cgctgaagcg ggagtatgtg
    13621 gattacgcaa gaatattcaa ccccgaacac aggcagtata ccctgaccgg ggccgtgatc
    13681 agcgggctcg gggaagggcg ctactacatg agcatccctc actacaagga gcagttcggc
    13741 gggtgtctcg ggtttgaacc cttccccggt accttaaaca tcaggctcga tgcgacgagc
    13801 attgaggtcc gcaagcagct cgaccgtgcc gggtgggtcg atgttcccgg gtttatcgct
    13861 gatgaacgga cgttcggtgg tgcccgggtg cttccctgct ggatcagggg ccagaggtgc
    13921 gcaatagtcg agccctctcg cacccactac ccggaggata tcatcgaggt cattgccggg
    13981 tgtgaactcc gcaaaatcct gaacttaaac gataacgata cgatcgaggt ggagattacc
    14041 catgattgat gcagccatag aggccctcgc cagaggcgaa ttcgtcctgt tatacgactt
    14101 tgacaaccgg gaacgcgaga ccgattttgc aatcaggtcg gacgcggtca cgcctgccca
    14161 catccggcag atgcgcaaag acggcggcgg gctgatctgc acggcggtgc atcccgaggc
    14221 cgcaaagagg ctcggcctgc cgtttgcgca cgacatcctg cgtgcgtgca gcctcgtcga
    14281 gaaggacggc gaggtcccct acgacccgaa gaaccattcg tcgttctcgc tctgggtgaa
    14341 ccaccgggag acctataccg gggtcacgga ccgggatcgc gctctgacgg tcaccgccgt
    14401 cgcagaagag gtgcagcggt cgcttaacgg cggcgggcac gactttgccg cacatttccg
    14461 gacaccgggc catatggcgc tcctgcgggc cgccgaccgg ctgctcgatg agcggcacgg
    14521 gcagacagaa ctctcgattg cgcttgccgg catggccggg atcactccgg ccgttacgat
    14581 ctgcgagatg ctggacgacg agacagggtt tgcactctca aaggagggtg cgatggacta
    14641 cgcgaggaag catgggctcg tcttcatcga ggggcaggag gttctggacc ggtgggattc
    14701 tcgggggccg aaagaataag acccactctt tttaacttgc gggggtgaag ggg
//
LOCUS       DAJB01000075            3956 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_137808, whole genome shotgun sequence.
ACCESSION   DAJB01000075 DAJB01000000
VERSION     DAJB01000075.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_137808"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          677 a         1264 c         1216 g          799 t
ORIGIN      
        1 cggactcgcc ccatctttgg atcttgagtt ccggggcctt gatctgctca gcctggaacc
       61 tgacatgctg caggcgaggg agcgtgatgc cgaccgctga agtgacactc tcgatcgatt
      121 caatcatccc attgatgaga tcgatcacat tgttgatctg cgtctcgatg tactggacga
      181 tatcgcccca tacatcgtag ataaagttca ggatcccctg cccgacccac tcgatcccgg
      241 ttgcgatcgc gtcccatccg gcccccatga ggccgtttaa gacctgcatg aaccctttgg
      301 taatgtcgac cagagcctcg cctgcgctct cccaatctcc cgcgaggatg gacgcgaaga
      361 gtttggcgac gttcagcatg acatcgagcg caccggagta gatctgctcg atatagggcc
      421 atgcccattc gaacagcggc acgatcaccg agccgatcac ccactggatg aacgtggaga
      481 tattggccca cgcggccatg aatacggggg attcctcctc ccaccagtcg gtgaggtatt
      541 ggagtttgct ctggaaaaac tcggtagccg gggcgagttt atcctgcatc gtagagacga
      601 acggggcggc gtcctgctgc gcttgcccga gccagtccgc gaacccctgc ccgagttcgg
      661 agacctgatc catcccggct tcgagggtcg gcatcgcgtc ctcgaacacc ccgatcagga
      721 gatccccgac gggctcaagg gcttcggtcg ccttcgcccc gaggatatcc atccggtcgc
      781 cgagggtcat tgcatccttc gcggcaccga ggatcgtctc ttcggagccg ttcagcgtcg
      841 agacgaaatc cccgagatcg agccgcccct cccggatggc gagcgccata tcagcggctg
      901 cccgggagcc gaagacctct gccgcgacgg acactccttc gaggtcagac ggggctgact
      961 tcacgcggtc gatgaaatcc tgccacgcag cggtgctgtc ggtgagcccc cctttggcaa
     1021 ggtttgcgag cccgaccttc atgccggaga gggtcgtctc aatgttcaca ccctccttct
     1081 cgaacttgcc gagcatcgcg attgagtcct cgaaatccat cccgagcccg cgcagagagg
     1141 tgccgtactg cgtggagagc gtggcgagtt tgtcgacccc gatccccgtt gcctgcgagg
     1201 tcttgaagag gaagtcgagt gtctcgctct gatcctctgt cgcgatcttc caatccccga
     1261 acaggcgcgt cacggcttta atattccctg cgacgctcgt cccggtgatc cgggagagtt
     1321 cgaggaactg cgtcgccatg tcctgcaggg cggggccggt cagcccgagc cgggtgttga
     1381 ggtcagcgat cgcggtgccg atatcggcag cactcgcggg cacctccccg aacacggcat
     1441 cgaagtcctc cttaagcccg gtgagcgcct cacctgtcgc gcctgtcccc acgcggatcg
     1501 tcgcgtaggc tttgtcaacg gtgttcgcgg cgacgatgga cgctccgccg atagcggcga
     1561 ccgctgctgc gacggcggcg atcgggacta ccatcgacga gagggtcgct gcgaacccgg
     1621 atgcgaacgt cgcggtctgt gcctgtgcgg tcgcgatgcc cgtagtaaac ccggttgagt
     1681 ttaacccgag cgacgctaca agattaccga cattcagcgc cacgctgcac caccttgccg
     1741 ccgaacgcga gcgcgattcc ctttgcgata gccttcatgt gtccctccga ttgtgccggt
     1801 ttcgccgtag tgtccgggaa aaagtctttc cacgtcagcg gcggcgagcc cttcgaccga
     1861 tacggggcga ccaccattgc gcagatcgtc ccggcgcgga tgttctcggc ctgctgctgc
     1921 cgccgctcct gtgccatccg cccggcgatc actgctgcga tctccgcagg cgtcaggtcg
     1981 tagaggatgc gcggatcggt ccaatatccc gtcccggcgg cgatgtcgag atactcccac
     2041 atccacccgg cgaacggcct cagtttcccg gagcgtcacc ctgcgcccgc gccatcgctg
     2101 aggtcaggat gcccccgagt tcgttgatgt cgatcgcgtc gatgaggtcg aagtactgtt
     2161 cctctgtcag ctgcttgccg tcgatcctga cgcaggtgcg agccatcttg cagaggtcag
     2221 cggtagaggg ctccgcgccg atggtctcct ggagatcctt gagtttgcag ccgaacgcgg
     2281 tttcaatctc gattgcggag cgggcagaaa aacggataga gtaggttttc ccgccaaact
     2341 cgcggatggt ttcgggaatc atccttagga cgcctcgctg aagaccggcg cggtcttgcc
     2401 gtcgatccgc atcgtgaagg tccgctgcac cttctcgtcc ttcgggaccg cgatcccgac
     2461 cccggccacg aacgccgtga acacgaatgt cgaggagtcg gggaaggtga tcgtgtactg
     2521 ggcgctcgtg cctgcgttgt aggcagtcac gaggcggttg ttgctggtgt cggtctggag
     2581 gtagttgagc gtcaggtcga acgatccggc gtccttgagg ccgggcacaa aggtcttgta
     2641 cccgccggtg ccgtagaccg tatcctcgat ctcatctgcg gtgagcgaga gatcgccgat
     2701 agcgtcgatg ttcgagatgt tcccacttgc gtctgcaata gtcgttgtct tgccaatagt
     2761 cgtcatgtgt accactcgct gatatcaaat gcagccgcgc atgacccggg cggtgcctcc
     2821 gggtagtcat caccgggcga gagccactgc gagagatcgt agtagtcccc gcaccggcgc
     2881 gatgcagaga gaatcggctc gatcgtgccg tccggctgga atcggaacga gcggcgcagg
     2941 agatccgctt tcggctgctc ctgcccgagc gcgagcacat acgccctaaa cgagtaggtc
     3001 gagtgatccg gcagtatcag cagataatcc gcgatctcct gcccctcgta gcggtcgatc
     3061 aggcgggcga ccccctcgtc gaacggctcg tagttgagcc ggatcgtcac cggggcggcc
     3121 ttcttgagac cgggcatact cgtctcctgc tcgctcgtgc cgtacgctgt ggtgtcgatt
     3181 gcctgccggg agaggtcgat ctgcccgatc gcgtccacgc aggcgatgaa ctcgccctcg
     3241 gtgacgtcgt agaacagcgt gcctttgccg atcatggtgt gaaccactcc gagaggtcgt
     3301 aatatgctcc gcacaggccg acgaccggcg ctgcccgctc gcggatcgtg gcgaagttaa
     3361 cgctgaactc gtgcgtctcc cccgtgctcg tgctcgtctt gccgaggtag accggatcgc
     3421 ccatcgcctc gatggagaga taccgggtgt cgttgatcgg ccagttggcg agcccgtcga
     3481 gggcatcccg gatctggtct accttgtcgc gggcgagcag atacgccggg ttcctcacga
     3541 gcacctgcac ggaggggcgg tcgaggatcg cggtcgggcc atattcgtgg tctggtgcgg
     3601 ggccgccggt ctcgatcacg gtcatcgcgg tcgcgagatc ggggtggtgc ccgaggaaga
     3661 ggctgatccc cggcgtcccg atcccctgcg ctgcgaggta ggcaacgaag tcctctccgg
     3721 cgctcgtcac agtttcacct gcccgaggag tttctgcata tacggcgcat agtctttcga
     3781 gcactcatcg accgcgtgct ggaggaactt ggcctcgccg accttgtgcg ctactccggt
     3841 gcgctcatgc acggggattg catacgctcg cccatctccc cacgtcgcgc cgaacttctc
     3901 atacccgacg acctgcacct gcgtgtctcc ttccttgagc ggcccctcgt tgaaca
//
LOCUS       DAJB01000076            3959 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_40939, whole genome shotgun sequence.
ACCESSION   DAJB01000076 DAJB01000000
VERSION     DAJB01000076.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_40939"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2894..2938
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          860 a         1170 c         1247 g          637 t
ORIGIN      
        1 ggacgggcta ccggcagttc gccggcaatc tgcgacaaac aggtctgagg ctccccgaga
       61 gccccggaca ctcgcaggaa gccccctgcg caagcggggg gtagttcaca taaaccatca
      121 ggtggaccag gtgaacaaat actccttcgt caccagagtg cccgacgaac cgggagcgct
      181 gcaccgcgcg gccgagatca tcaagtcgta ttcgggcaac atcaaccgca tccagttcga
      241 ccgcaggatc gatcccgtca ccgtcttctt cgaggtgacc gccacaccgg aggggtactg
      301 ccggataaag gaagacctcc atgcgatcgg ctacctccag gaatcgctcc ccgcgctcgg
      361 gtttctcagg ttctccgtat acctccccca cgaaccaggg tcgctcttca aactcctcac
      421 ctacatcacc ggggcggggg cgaacatcgc ctacatcgat ttcgacgacc ggcggtgcga
      481 ccccggccgg gtcaccatca gtttaaatgt cgaagagacc gcgatcgtgg agagcctgct
      541 cgaccggctg aaatcacagt accgcctcga tatcctcgag tacgacacga ccggcgagaa
      601 gctcgacgac accgtcttct atgtcaggtt cgcccaggcg gtgcgggggc tgatcgggac
      661 ggccgaggac gagttcctgc tctccctcct ccaggacgtc aaccacatcg tccaggaact
      721 caacagcctc ggtcaggacc caagagaggt cttcgagtgc atcctcctca cgggaaggac
      781 gctccaaaac actaccggag accggttcta cgcggatgtc cagcgcatcc gcttgagcga
      841 cgacgtcgag gtcttctgct tccagacgcc tggcggcggg agcatcttcc tgctccgcgc
      901 tccggaggag accgtcatga tcgataccgg atacgggatc taccacgagg atgccgtgcg
      961 gatgttccag cactacggcc tcggggatct caaaaagatc aagaggatct acatcaccca
     1021 tgccgacgcg gaccactgcg gggcgggcgg gctctttgac gcaacatcgt atacgcacac
     1081 cgggtcgcgg gagatcatcc gccgggcgaa ccgggcctac gggtcgcgca gcgagtcctc
     1141 gatcctcgaa gaggtctaca cgaccgtcat caacctcttc tcgcacttca ccccgccgga
     1201 gaacctcgaa gttttcccgg aggagaagat cggcacgcgc tcgatcttcc ccatccttgc
     1261 ccgctttacg gtccacgacc ttgagttcga ggtccttgag tccctggggg gacacatgca
     1321 cggccaggtc tacctcttct gtcccgggca cgggatcgtc ttcaccgcgg acacgctgat
     1381 caatttcgga agcctcgatg aggaccggaa acgctacaac tcgctggccg acttcctggt
     1441 gacgtcggtc aacgtcgaca gcgactgcgc gcggcaggaa cgcaaggcgt tgcttgagat
     1501 gatccgggac ctcgatgagg aacttgcccc gaccggccgc cggtgcctgg tcgcctgcgg
     1561 ccacggcgcc atctccgtcc tgaaagatgg taaactggaa gcgatcggcc cggtcgagcg
     1621 ttaccaggcg gagaagaggg gagtttcggc ggtcgtccgg gggcaccgcc cccacacccc
     1681 ggcggtcaac tgcccggcag ggtggaccgc cccgggcctc gatttttttc tgccgtcacg
     1741 ccgccccgcc ggactgccgc tccccgagca atgcaatccc tgcaccgaga acgaccagcg
     1801 ctccgccgac cagcagcacc agggagtcca gctggacgag gaggaagagg cttgagacga
     1861 tgccgacgac cgggatgatc gggagagacc ctatgctgcc cggtatccgg aagggccgga
     1921 cgacatccgg ggcacggtac cggagcagga tcacggatgc gttgatgaaa acgaatgtca
     1981 caaagagcgt gaagttcgtg atgttcgcga cgaaatcgat ctcgccggca aagaggaaga
     2041 ccatggaggc gagcgcaacg gcgacgatgg ctgtccaggg cgttcgtgtc cgggggtgca
     2101 cccggccgag gaccgacggg agcgagaagg aggaggccat gccgtagatg atccgggacg
     2161 acgccatcat catcaggagg gcggtgttcg cggtggcaaa gagggcgatg acggagatga
     2221 tggtgaacgc tgccggaccg atggcgacag ctgcgacgtc ggcgaagggt gcccgggagg
     2281 cggcgagttg ctcggcgccg acgaccgaga cggcggagag cgacaccagg atatagagga
     2341 cgacggagat ggcaagtgcg atgatcaggg cgagggggat ggttcgctcc gggttccgcg
     2401 tctcctcgga gagttttacc atctcctcaa accccatgta ggcgaagaag acgagtgcgg
     2461 acgcctgcag gagccccggg actccctgcg gcatatcgag gtaatccacc tgcccgagat
     2521 acgggaggcc gatcaggatg accatgacga tgcctccaac ctcgatgagg gtcagggcga
     2581 tggcgacccg ggcggtctcc ttgatgccgt agagaagtat cccggcgagg acgagtatga
     2641 tcagaattgc tgaagggatg accggaaagc cgaccagatc cgagaagtac ccggcaaacc
     2701 cgagcgagac ggttgcggcg ccgaggatgc cggagaagat gatcagccac ccgacgacga
     2761 acgcaagcct ccgcccgaag gcgttcgaga catactcgaa ctcagcgctt gcacggggat
     2821 acatcgacga gagctcggca taggcgaggg cgctgaacgc ggcgatggtg gcggcgatgg
     2881 cgaacgtcag ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     2941 ccgtttcctc ttccagcata cgcataaccc tatgtgcctg caccatttat gggtatccgg
     3001 caggttctgc cgcaccgctt cacgcgtaga ggagaggacg tttccagaga cctccgggtg
     3061 ctttccccgg gattcggaag agaatcgatc ctgcacatcg ggcacaccat acgtttttat
     3121 cagatgcata ccccgggggc acccatacgg gggatagaga tggggcagaa catactcaat
     3181 ctcttacggc aggaacatgc gaagatgctg atgcaactgg gcgaactcca gcagagggag
     3241 atccgtgacc gggcagagtt gttcaaagag atgaagaaca cccttctgcc gcacatggcc
     3301 ggcgaggaac gggtattcta tccgcggctc gaagaacagg ggctgcatga cctcgtgaca
     3361 gggtcgcggg aggagcataa gatcatccag gcgctcatcg accggttaaa caacatttca
     3421 acggccgatg aagaggactg ggtcgacgtg atgtcgcaac tccaggaggc ggtgaggggc
     3481 catgtcgacc gggaagagaa gacggtcttc ccggaagcca ggcagaagat agacgatgcc
     3541 ctcctcctgc gtatcggcaa ccggttcgag gaggcgaaag ggaaggccca cctgcgaccg
     3601 ctcaggtgat tcctgcggtc tcccgggcgg gtgccgggat cccggatccg ggtataattc
     3661 atatatatcg tgactgccca tgcaggcctc cctgtcaggg gggagtgatc agatggcgca
     3721 gaatgttatt gatatcctga agcaggagca cgaaatggtg ctctcgcaac tctcggaact
     3781 ctcaagcaag ggcacgagca accgtgagca gaagtacaac tccttaaagg agaacctcat
     3841 cccgcacatg gtcggggagg agcaggcgct ttacccgagg ctcatggagt cggggatgca
     3901 ggagatggca cttgaggcga tcgaggagca caacgcggtc aagacgctgc ttggccagc
//
LOCUS       DAJB01000077            4012 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_63959, whole genome shotgun sequence.
ACCESSION   DAJB01000077 DAJB01000000
VERSION     DAJB01000077.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_63959"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1755..1782
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          821 a         1254 c         1181 g          728 t
ORIGIN      
        1 cggtttcaac cgggggtagt tgacgtgctg gcatcgcttc tcgaggatct cccgggtgcc
       61 gatgtctctg cggtaaaaga cgcgccctga gacagaagat gccgccttct cggctattgc
      121 ttcggcctcc tcgagcgtct cccccctccc gacgaacgcg agcgtccgcg acgtctgggt
      181 atagagcgtc ccgtcccgct cttcgacgtt tgcgtagtaa agcagggcgt cgccgtagtc
      241 gccgatggtg agaggctggc gggtcaccgg agcctccggg tagccctcgg ggacgagata
      301 cttgcagacc gtcgctttat gagcgaaccg gacatgtgac ggcgagaggt ctccctcgat
      361 gatatggcgg acgatcgcgg agaagtccga ctccaggagc aagaggacgt tcatcgcctc
      421 agggtccccg aagcgggcgt taaactcgat gaccctgggc ccctcgcggg tgttcatgaa
      481 ctggccgtaa aggatcccgc ggtacggcgt cccctcgttc cgcatggccg cgaccgtatc
      541 ctccatgatc cggagcgctt tctcgtagtc cgaccgggtg acgaagggga gcatgtggtc
      601 cgccagggag tatgacccca tgccgccggt gttcggcccc acatccccct cgtatgcacg
      661 tttgtggtcc tgcacgagtg gcatgggcac caggtgctcg ccatcgacaa acgcctggag
      721 ggtgaactcc tccccgataa gccgctcttc cagcacgacg tcccctccga tctcccgggc
      781 gtactcgact gcccccgcca cgtcgacgtg ctcgcccatg atccgcacac ccttcccgcc
      841 ggtcaggccg ataggcttga cggcgaggtc gccattgtaa gcctcgatga accgccgtgc
      901 ctcctcgggg tcatggcaga cccggtattc cgggcacccc gcgatcccgt gccgttccat
      961 cagccggcgg cagaacgcct tgtccgtctc aagccgggcc gcggcacggg tcggccccat
     1021 gcagggtatc cccgccgcct cgaggtggtc gacgacgcct gcctcaaggg gcgcctccgg
     1081 gccgatgacc gcagcgtcaa ctctgcaatc ggtggcgaat gtaactatct tcgggatatt
     1141 ggtctctttt tcgaggagca cccgctccga gagccgggca atccccggat tcttgcgtgc
     1201 catgacagaa aatattctca tgtcactgtt gcaggaaagc gctctggtga tcgcatgctc
     1261 cctgccacca ccgcctacaa cgagtacttt catatccatc tattcgcctg ctcgtaaaaa
     1321 aatgttcacc cgtccatcag ttcgctgacc cgcacaagcg catgaagtga tgcgcctttt
     1381 tccgcaagtg cctcatgggc gcctgcttcc cggtcaacga cggtcacgac caggtcgacg
     1441 tgcgcgcccg cagcacgcag cgccgcgagg ccgtacaggg cgctcccccc ggatgtggtc
     1501 acatcctcga ccagcagcac gttcctgccg ctcacttcgc cgatgatcgt cccggcttta
     1561 ccgtggtcct tctctgcttt cctgatgatc gcgaacggcc ggccgctcgc gagcgagacg
     1621 gccacggcga tcggtacggc gccgaccgcc accccggcca ccatatcgaa ctcccactct
     1681 tcggcaatgg cttttccgat tgcggaaagg atggcgggct tcgtcgttgc ggctttgatg
     1741 tcgatgtagt agggnnnnnn nnnnnnnnnn nnnnnnnnnn nnatccaggc cacaatgggt
     1801 agggtaatat tcccaaacaa ccactccgga taaatcagga ggatgagcag gaaggaaccg
     1861 atgaccaggt cgtactgatc ggccaggaac caggattcgc cccgcccttt acccagtctc
     1921 cgcttcagga agctcttggc gagatcaccg aggagcgcgc ctgtcgcgag cagcgtgacg
     1981 gagaggatgg tctgcatggg gagtgcggtc aggttgaacg aggaggcggc ccagatctcg
     2041 acgagacccg ccagtattcc acagaggaca cccccgaaga acccccggta agtcttaccg
     2101 tcgccgaaga ttcttcttcc gtcaccagag gtcctgccaa agtcgatggg cgtcccgccg
     2161 ccgaagagtg cggccgctga gttcggcacg tatgccggaa tcatgatcca cagcgccgca
     2221 agcccggaga agatccagta tccgacgtcc gcatcgataa gcaccaatac tcagtacaat
     2281 cagtaaatat aaagattaaa gtgtcgaaat atgagatacg aatgaataga tgggcacggt
     2341 gagagaatgc tgataaaaaa gacaaagagc ctctgcccaa catgcggtcg tgtgctcgac
     2401 gccgatatcg ttgaggaaga ggggaaggtc tggctggacc gtacctgccc tgaacacggc
     2461 acgtaccggg ccctctactg gtcggacgca gagatgtacc ggcgattcga cgcctacgaa
     2521 cgcataggga agggggtttc gaacccgcag aagaccgctt ctcaggaagg gtgcccgacc
     2581 gactgcggca tatgtcagaa tcaccggtcg acgacgttgc tcgcgaacat cgacctgacg
     2641 aaccggtgta acctcaactg cgacttctgc tttgcaaacg cccgggcatg cggttacatc
     2701 tacgaaccga ccttcgacca ggttgtcggg atgctgcgca tgctgcgcga ggagaagcct
     2761 gttccggcgc ctgccgtcca gttctcgggc ggcgaaccaa cgatgcgcga tgatcttccc
     2821 gagatcatcc ggaaggcgaa agaactcggg ttggcccagg tgcaggtcgc gacaaacggc
     2881 atcaggctcg cacaggagcc cgactacgcc cggcagctga aggaagcggg gctctcgatc
     2941 gtttacctgc acttcgacgg cataacccgc gagacgaact cgaaactcgc gagcgatcgg
     3001 cgagccatag agaactgcga gaagatcggc atgggggtgg tgctggtgcc cacggtcatc
     3061 aagggcagga acgaccacga ggtgggcgcc atcatcaagt acgccgcaga gcgcatcaag
     3121 atcatccggg gcgtcaactt ccagccggtg tcgttcaccg gggctgcaag cgaggatgat
     3181 gtcaggaaag agcggatcac catcccggag cttgcagaac ggatcgagga gcagacggac
     3241 ggcgtgatca agaaggacta cttctacccc gtgccctgcg tcgtcccgat ctccgagctc
     3301 gttgaggcat acacgggcaa accccagatt acgttcacca cgcaccagca ctgcggcgcg
     3361 gcgacctacg tcttcgttac cgacgagggg atggtcccgg tcaacaagat gttggacgtc
     3421 gacgccttct tcgagtcgat cgagaagatg acggcgaaac tcgggaaagg cgggtcgctc
     3481 aacaagtata tgacccttgt cgagggggtc aaggacctct acacctccac gaggaaggcg
     3541 gagcagaaga ataccgggga gttcctgaag ttgatcggaa agaccctggt gatgcagaac
     3601 ttcgaggccc tcagcgagtt ccactggaac gccctcttca tcggcacgat gcacttcatg
     3661 gacaaataca actacgacct ctcccgggtg cagcggtgct gcatccacta cgcgaccccc
     3721 gatggccgcc tgatcccgtt ctgcacctac aacagcggcc cggtctaccg cgagcaggtc
     3781 tggaaagcct tcgcgcagcc ccagaaggac gagtgacggc ccaccataca catctcgggg
     3841 agggttcacc gtcccgtccc cgggcggctt ttcttcggtg cctcacgctc cagttctagc
     3901 gaaccgtcgc gtcctacgga cagtcgttct caggaatgtt gcaccttcgg ccagctatgc
     3961 tctgcgccgt caccactcgc accagcggtg ttcataataa tcaggcagga ga
//
LOCUS       DAJB01000078            6000 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_219647, whole genome shotgun sequence.
ACCESSION   DAJB01000078 DAJB01000000
VERSION     DAJB01000078.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_219647"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1029 a         1828 c         2090 g         1045 t
ORIGIN      
        1 ggatctcgca gtactggacg ttgataccgt tcgcggccag cgcatcggtg atctcctcgg
       61 actggtaggg cgggtagagc cagtcggagg agaccgagac gaccaggaac gaggccttcg
      121 tcccggcaaa accggcggcg agcgagtcgt ccacggcgag gtcgaagtag tcgagcgccc
      181 gggtgatgta gaggtaggag ttggcgtcga accgccgggt gaaggtgtcg ccctggtggt
      241 ggaggtaact ctcgacctgg aactcggtgg agaagccgta gccgacagtg ctgcggccct
      301 gcaggttccg cccgaacttc tcgcgcatcg ctccgtccga gaggtaggtg atgtgcccga
      361 tcatccgggc gagactaagc ccctgcaccg gggggcgttc gcccgggtag cccccacccg
      421 cccacgcggg gtcggccatg atcgcctgcc gcccgacctc gttgaaggcg atctgctggg
      481 cggtcgagcg tgcggtcgtg gcgatggcga tcaccttccg gaccgcttcc gggtaggcca
      541 ccgcccactg gagcgcctgc atgcccccca tcgaaccgcc tgccaccgcg gcgaggcagt
      601 cgatcccgag atgctctatc agtcgtttct gcgcccggac catatcccgg acggtgatca
      661 ccgggaacgc cagagcgtag ggccggccgg tcgccgggtt gacggaggcc ggcccggtcg
      721 agcctttgca ccccccgatg acattcgagc agacgacgaa gtagcggtcg gtgtcgaagg
      781 cttttccggg accgatgacg ccgtcccacc acccgggccg ggtttcgccg tcgtgaaaac
      841 cagccgcgtg cgcatccccg gagagggcat ggcagatcag gatcgcgttg ctcttctccc
      901 tgttcaaccg accgtaggtc tcgtaggcga tcgtcaccgg cgcgataacc gcaccgctct
      961 cgagggtgag gggttcgggg aaggtggcgt gctccgtcct cacggtaccg acggatccgg
     1021 gcatgttacg catcccgcag agcctggtcg aggtcttcga tgatatccct gatatcctct
     1081 atcccgacgg agaggcgtat gtagtccggg gtgaccccgc acgccgcctg ctccgcgggc
     1141 gtcagctgct ggtgggtggt gctcgccggg tgaatgacga ggctctttgc gtcgccgatg
     1201 ttcgcnnnnn nnngtagttc cgggcgacgg cgtgggccgg gtgatcgggg aggccggggt
     1261 agttcaccca ggcgaccttc gggtggtccc ggaggaaccg ggcgacgcaa agggcgttct
     1321 ccgagtgccg ctccatccgg aggtggagcg tctcaagccc cagcaggagg aggaaggcgt
     1381 tcatcggcga gaggcacgca cccatgtccc ggaggacttc aagccggacc ttgtaggcga
     1441 gggcgacgtt cccggaaccc gggtagtcac ggaacgtctc ccagtattgg agcccgtgat
     1501 acgagggatc gggctcggtg aacccgggga acttcccgtt atcccaggcg aagtttccgg
     1561 aatcgacgat caccccgccg atagaggtgc cgtgaccccc gacgaacttg gtggccgaga
     1621 ggacgacgat atcggcgccg tggtcgatgg ggcggaccag gccgaccgcg gcggtgttgt
     1681 ccacgacgaa cggcacgccg gccccgtggg cgatcgcagc gatggccgcg aaatcgggaa
     1741 cgtccagttt ggggttcccg atcgactccg cgtagatcgc ccgggtcttc tccgttattg
     1801 ctgatcggaa ggcctccggg tcggtcgaat cgacgaagac caccctccgt cccagtttcg
     1861 ggaaggtgta gttgaagagt tcgtaggtgc cgccgtagag gttatcagcg gcgacgatct
     1921 cgtcgcccgg ccgggtgatg gcaaggaggg cgttcgagat cgccgccatc cccgacgcga
     1981 ccgcgaccgc tccggtcccc ccttcgatgg cggccatccg ctgttcgaag acgtcggtgg
     2041 tgggattcat cagccgcgtg tagatgttcc cgggttcttt gagtgcaaag aggttcgccg
     2101 cgtgctccgt gtcacggaag acgtaggagg tcgtctggta gatcggtact gcccgggctt
     2161 tcgtcgccgg atcgggcgat tggccggcgt ggagggcgag cgtgcccggc cgatagtggt
     2221 gcgtgctcat gcttattctg tccttgttgt aagatcatat tttgcggcga tctcctggaa
     2281 ggcctccgtc aggtaccgga tcttcttcca ggagaggccg aaggtgttca acttccaggt
     2341 ccgggtggcg ccggcaaact ccccggcgat accccgccgg gagagctcgt cggagaggaa
     2401 gaagccccgc cgcttgtggg tcgcggcgac tgcatcgaaa ctcccggtgg tgtccacctt
     2461 ggtgagggtg tgcttccgcg ggtactcgga gaggaccttc gtcccctcga tggcaaggag
     2521 gccgtcgatg aagtggttgg acttcttcac ctcctcatcc cagtggaggg tacgctcccg
     2581 gacggtcggg aacgaggcga tcatggcgat gaggttcgcc cccatcaggg tgcacccgag
     2641 catctccacc tccttgaccc cgaacttccg gtgggtgaga tccccggtca tcgtcgtggt
     2701 ccgaaagacc cgcggcgccc actcggcggt ggcggcaagg accccggagg gagcggggga
     2761 ggccatgctc ttgtgcccgg agccgacgac gaaatcggcc ccgatcgctt tgccgtcgac
     2821 gggcatggtc ccgaccgtgt aggcgccgtt gaggaggaag ggaacgtcgt actggtgagc
     2881 gaccttcgcg atgccggcga cgtcgtgaac gttcccgaac tggtagtcga cgtggtcgat
     2941 catgatgagc gctggattcc gcccggtctc acgccggacg tcctcgattt tctcggcggt
     3001 agcgtcggga gtgacgaggt tctgcttcga gagcccgacc tccttcacca cgccgccagc
     3061 cccttcgacg gcaaggaact ccgtgtagtg ggcgagagat gagacgagga ccgggtcgcc
     3121 cttctccacg agcgtggagg cgaccgcctg gaacccccgg cgggcgccgg gaacgacgcg
     3181 gaccacgtcc atgttcagga accgggcgag atcctcgtgg aacgccgcga tggggggctt
     3241 tgtgatcttg tcgaggcgga acggctttcg gcaggcatcg caggtggagt agccgtcgcc
     3301 gtaggcgatg agcgccttcc gggcttcgag agtcaggcgg ccggcggcct ggatgggctg
     3361 gatgttgata tatgcctctt cccggttccg gatatcgagg ctgcccgcga tcttcgtgac
     3421 cgcaggggtc ccggtccccg cctccaggtc gtcgaggatg gcccggaccc gggcgacacc
     3481 ctcgcggatg gctcgctcct cgtcgggccc gggaccggtc gggagcgcct cccggagcac
     3541 ctcccggata tcctcgagcg caaagagcgc ttcgaacgtc ttctgaacct gttctttcat
     3601 ggggatgcct caagccgagg cccggagtcg aaccgggatg gagctgatct gcagtcaacc
     3661 gcgtagccgt tccgccacct cggcaggatg ttggattaac aattgttaat cttacccatc
     3721 atagataaca attgtgaata tttatacctt agggtcacgc catgcgccgg cgaggacgga
     3781 aacgcagtgc cctccccggg gaggagagta aagaggcagg ccagaccccg atgtaccggc
     3841 caaagatgca cgcgcaggtc agaaggccag gtggacgagc cccatcgtcg ccgccatgac
     3901 cacgatgacc gcgattatcc atccggcgag catgcagagg agagaggtgc tcctcgggta
     3961 ctggaagagc cacgcgacga caggggcacc gtagagagcg atcccgggga tccatatgac
     4021 cgggacgagc atcagggcac cgtaacgctt caaggattca attctcccgg tcttcgcgtc
     4081 gatattccgg agaagcccct ggataaatcg agagcgctcg gcgaagaggt cgcagagttc
     4141 aaggatgccg agcatgacgg cggccgccac cgatgtcagg aaagcgagga cgaccgcagg
     4201 gtggagcccg agcgagaggc cgacgacggc tgcggccgcc tggaggagga gggtcgatgc
     4261 gatgagggcg aagatcgctg gcggaggtat gccgaaggca aggccgagga ggagcgggac
     4321 cgccaggagt aaaaggagcg cgaacgcggc ggctcgcacg atccgcatcc ggcaggtgcc
     4381 cggggagagc gcccgctcac atgcctcgat ggcagcaacc ctgagcatta ctctccttct
     4441 ggaccctgga ggtagatgaa cctgtgcacc ccctgcggag accggaggtg tggtacaaaa
     4501 aagccagcta tttattccgg gcggacctac acccacgttt atgagcgaga cagaaggtcg
     4561 cttcgagcgg gggcggtggg tcgttgatcc tgcccccgta ccggagaagg agccggcacc
     4621 ggcagcccct accgttgatg agcatgtgca cgaggcatcc agctcggtcc ggaaggcggt
     4681 caatgatgtg atgaacgcgg gccaccacct cttaggaacg cccgaaggac acgaacgtat
     4741 cgagcaggcg gcacggaagg ccggggaaga cctggagcgg gcggtcaact cctgggcgga
     4801 gtccgcacgg caggccttag ggagacggtg agaggcccga gaggtacccc gtgacgcggg
     4861 cgactacctc ggggtttctc ggcaacatgg tgtggcagta gtgctccggg tgccgggcca
     4921 ggttcccggg gtctgccggg agcacgtcca cccctgcccc ggggaggtat gagtcggtgt
     4981 gggggacgat gccgtcaccg gcgtaggtcg tccgccaccc tccgtccgga ccgatctccc
     5041 aggttttccc gccgaaggcc gggaagaacg ccggggtcgc ggtgaggttc gcggcgagga
     5101 cgatccggta ggcgatatcg gcgcgggtcc cggcgtcccg gagcgccgca acggtcctgc
     5161 tcccgggccg aaactcctgg acgatggtgt cctcccgggg gtcgtagtcc tccggcacga
     5221 agacccctgc gaggcgccgg ataacctccg gaccccgttc ggggtcgttg aagagttcgg
     5281 ccatggccga gccgttgttc ggcgggccga tcccgaccag gagccggacc tgttcctccc
     5341 ggtcgtcgcc gtcgaggact tcgaggaggt accgggcgat gcacgtcccc atcgagtggc
     5401 agacgacatc gaccgggccg tcgtacccgg tctcctcccg ttttgcgcgg atgtagtcct
     5461 ggagagcgag ggcgatcgcc tccgtcccgg cgctctgcat cgccgtgtga tcgaacctcc
     5521 agaaggggac cgcggcctcc gtgagccccg gggtgaggcg gttccagatg ccgggatggc
     5581 ttcgccaccc gtggaccagg acggccgggc atctgtggtt cgtcaggatg gtaccacgcc
     5641 tccccgcggc aagcaacggt tcatttatcc gtgatttgat gctatccacg atgtaggttt
     5701 cggcggctac cctaccacca tcttgtttgc cttcaggaag aagcccggcg gcatcgggcg
     5761 gtcgagtttc caggtgatgc tcatcggccg gctcccttca tgggagagat accgggcggt
     5821 cccgaggaag agatacggct gcgtgacgcc gtcgaccctg ttgtactcgc ggacgaagag
     5881 gaggatcttg ttcccctgct gttcgtgctc gatgtagcgc tggccggtcg gtgacgacgc
     5941 ggatgtggtg ctctgcgact gccagtggaa cagttcctcg ttgaccgcgt agtctttgta
//
LOCUS       DAJB01000079            5686 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_140207, whole genome shotgun sequence.
ACCESSION   DAJB01000079 DAJB01000000
VERSION     DAJB01000079.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_140207"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1067 a         1744 c         1848 g         1025 t
ORIGIN      
        1 atcggcagtt gctggatcgg ctccgccgcc ctcgtccagc agaaccccga cctcctcgcg
       61 gcgctgaagg tcccggacga tcaccagatc gtggcgccgc tgatcttcgg gtatcccgcc
      121 cccctcctgc aaaaaccgga acggcgggaa ccccggatca cctggatccc ctgaccgccc
      181 ggcgttcacc cggtcgcggg acacacaacc gcttccgccg ggacgatcgg gagcgtgatc
      241 cgccgcgctt cccgaacaag cgtcagggtt gccggctccg cgatcggcca ggtggcgagg
      301 aaccggtgca ggtcgtccac gcacgcaacg tcggtctcgt tgattgcgac gatcaggtct
      361 cccggccgga gccctgcccg accggcgggg cctgcccggg tcaccgagac gacctcgact
      421 gcgtggctcc cggtaagccc gagtttcagg acgagcggct gctccagcgg ccgtgactgg
      481 ccggcaatgc cgaggtatcc gcgccgcacc ctgccgtcgg cgacgagctg cgggaggacc
      541 caggctgcgg tgttcgaggg gatcgagaag cagattccct gggccgtcgg gatgatggcc
      601 gtgttgatgc cgatgacccg gccgcgcgag tccaccagcg gaccgccgga gttgccggga
      661 ttcaaggggg cggtgtgctg gatgatgttc tcgatcaggc ggccgtcccg gctccggagc
      721 gcccgcccga gagcgctcag gactcccgtc gagacggtcg agtcgaagcc gagcgggttg
      781 ccgatggcta tcgcgagctg gccgaccgcg agcgtccccg agtcgccgaa cggcgcgtag
      841 tcgtggagat agagaccgac cgggggacga ccacctgccg ctactacgag gccgaggggg
      901 caagaaatgg tgtcgtcatg gtcggcggga ccgggggcgg gttcgacacc ccggtcggga
      961 tctctacccg cgactggcaa aagacctctc agatgacagg atcagcacac tccgggtgca
     1021 gtaccggcat ccgaccgacc tcgtggagtc ggccgtcgat acggtcctcg gcacgcgcta
     1081 tcttgaaagc cagggtgtct cagccgtcgg gctggtcggg cactcgctcg ggggagcggt
     1141 ggtgatccag gcggcggcaa gcgaccccgc ggtcagggtc gtcgtcacca tcgcgacgca
     1201 ggcctccggg accgaaccgg tcgccggcct cgccggccgt gcggctatcc tcctgattca
     1261 cggtgatgcc gacagagtcc ttgctcaccg ctcttcagag gaggtctacc gccgtgcccg
     1321 ggagccgaga aggctggtcg tctacgaagg agcgggccac accctcgatg aggtggcgga
     1381 gagcctctac gtcgacgtga aggcgtggct cctcaaacac ctcccgggga cgctcgtctg
     1441 accgggcggt ccgccgctcc ggcctggagg tatcgccgtg cggcgcacgg gtcgtcgctc
     1501 gcgaaaccat cgacgccgag tcgtgccgcc gcttcgatat cctcctctgt attcagcgtc
     1561 cagggaacga cgagcagccc ggcatcgtgc gcccgctcca ccacttcggg cgagaggagg
     1621 tcccaccgcg ggaggatgac gtccgcctgc accgagcggg cgagcgcgac gggatcggca
     1681 gtctcccccg aatagatgag gcccgcctgt gctccgggga ggagtttctt cgccatcgca
     1741 acgcttccgg ggtgaaacga gacaaggaag agctgttccg gcatccggtc catgagcact
     1801 gaagcgatga tcgcttcggt cccgggctcc ttgatctcca cgacaagccc ggtctgtccg
     1861 tggacaaggg cgaggacctc tccgagcgtc gggatactct cgccctcacc cgcgtccagc
     1921 ctcgttaagt cctcgatggt atagcctttg accggcccgg tcccgtcggt cgtccggtcg
     1981 acggttgcat cgtggattgc gaccgggata ccgtcccgtg tgaggcgcag gtcgacctcg
     2041 acgaggtcgg cacacgccat acccctccgc agggcccgca gggtgttctc cggttcgaga
     2101 cctctcgcac cgcggtgtcc gatgatgagc atgccacgcc atgcaccccc cgaccagata
     2161 taggtggcgg gtcgcggggc cggtattggc ccgacgtttg ctgtgaaaag agttacttaa
     2221 cccttccagg ttcccggctt ccgtccgctg cagaccggta cgacttctcc tggaacatct
     2281 tgacgaacgg acggtctctc ttcctccggt atctccactc acttctgtcc accgtgcacc
     2341 tgcataccgg acgcacgccc tgcaggggcg ccaccggacc aaacggtttc ggtcggccgg
     2401 gagtaagagc cggcaggtgc cggagccggc acctgcggaa aaaagttact tattctggat
     2461 cttgttgaac tcggacttca cgtcttccgg atcggtatac tgccggtctt cgaactggtt
     2521 gagcgcatcg agaacgatct gaggcgcatc ggtccccttc gcgcgggaaa taaggtcgtt
     2581 cttcgatgcg gggaagttta tgccggaggt gctcacctcg atgatctgtg ggtcgagttc
     2641 ctttatggtt ccggcgactt ttgacatccc gcccatggcg gattctttca tgcctttcat
     2701 agttctcacc atctggtctc tgctcccttc cgggagacga cccctggtgg ataaaatgta
     2761 ataaatatct atcgcccgca cggcggcaag ggtcaccccg tatcggccgg accgctctcg
     2821 accggggaga gccttgaggt taggccggag gttctggggc cgacctgtgc acccgctcca
     2881 ttctcccggg gattccagca acacttaagt aggtgaggat ggggaagaat gagtatgcca
     2941 cctgcacccg aaatcaggat ccctctcgat gtgccccccg aggagcagga gaggtaccgg
     3001 gagaactatc ggacgatcac gcacgacacc gggagactga tgctctttgc cggggaccag
     3061 aagatcgagc acctcaacga tgactttttc ggcgaaggca tccaccccga ggatgccgac
     3121 cccgaacacc tcttccggat tgcgagccgg gcgaggatcg gggtctttgc gacgcaactc
     3181 gggatgatcg cccggtacgg tgcggattac cgggacgtgc cgtacctcgt caaactcaac
     3241 tccaagacga accttgtcgg gacgtcccag cgggacccgc tcagctcgga gctgcaccac
     3301 gtccggcagg ccgtcgacct ccgggacaag acggggctct cgatcctcgg ggtcggctac
     3361 acggtctacc tcgggagcga gtacgagccg atgatgctct accaggcggc ccagatcatc
     3421 aaccatgcgc accagaacgg cctcgtcacc gtgctctgga tgtatccccg gggcaaggcg
     3481 gtcaaggacg agcgggaccc gcacctggtc gcgggcgcgg cgggtgtcgc ggcgaccctc
     3541 gggagcgact ttgcaaaggt gaatccgcca aaggtgggca agtcggtcga tgctgcgctg
     3601 ctccgcgagg cggtggcggc tgcaggcagg acgggcgtgg tctgtgcggg cgggtcgcac
     3661 gccagcatgc cggagttcct gcagcagctc cacgaccaga tccacatcgg cggcacggtc
     3721 gggaacgcga ccggcaggaa catccaccag cggtcgctcg acgaagccgt ccggttctgc
     3781 aacgcgatat cggcgatcac cctcgacgat gcaagcgtgg atgaagcaaa caagatctgc
     3841 gtggaaggca ggtgctagga tgacgacagt cggcgtactg acgggaggag gagactgccc
     3901 gggcctgaac gcggtgatcc gggcggtcgt gcgggcgggg gcaaaacacg ggttcgacac
     3961 gatcgggatc cgggacggat ggcacgggct ggttgccggg aacgtcgagc cgctcaccga
     4021 ttactcggtg accgggatcc tccccaaggg cgggacgatc ctcgggacgt cgcggacgaa
     4081 ccccttcaag agcgaggccg acgtccagcg gctgcgggac aacatcagga agttcgggat
     4141 cgatgcgatt gtcgcgatcg gcggcgagga taccctcggt gtcgcgaaca aactcgcaaa
     4201 gatgggtatc ccggtggtgg gggtgcccaa gaccatcgac aacgacgtcg gcgcgaccga
     4261 ctacacgttc gggttcgaca ccgccgtcgc caccgtcacc gaggcgattg accgcctcca
     4321 cacgacggcg gagtcgcacc accggatcat ggtggtggag gtgatgggcc ggcatgccgg
     4381 atggatcgcg accatggccg ggatcgcagg cggggctgac gagatcctca tcccggagat
     4441 cccgttcgac ctcgatgagg ttacccacca tctccgggca cgctacgaga agggaaagaa
     4501 gttcagcatc gtcgtggtcg ccgaaggcgc ccagccgaag ggaatggcgg aagcgatcgc
     4561 acaatccgcc cggacggacg agttcggcca cgtgatgctc ggcggcgtcg gcacgttgct
     4621 gcgcgccgaa ctggaaaaga ggctcgatat ggaggtccgg gtgacggtcc tcgggcacgt
     4681 ccagcgcggc ggttctccga cggcgcacga ccgggtgctc gcgacccggt tcggggtggc
     4741 ggcggcggac ctcatcaagg agaaggactt cgggaagatg gtcgcgctcc ggggggacga
     4801 tatcgtcgcg gtcccgctcg aggaggcggt ggcgaacctc aagaccgtgg atatggacct
     4861 ctacaagatg gcaagcatct tttatggggg gtgacggggg cctctctttc gaaccccacc
     4921 ccgcccgcgc ttcgcagtac acttcttccc agcgaaaccc gggtgggagc cggggtgagg
     4981 gggcattctt tgcccggaag cctgccccct gatatccttt caagattcaa gctcgtccga
     5041 tcgccgggag agactccttc gaaaattgag ctggaaaaag agatgagggc ataattgtcg
     5101 atttccatga tcaaacctat aattcccagg atttcgcgat tgttccggaa caatcccctt
     5161 tctgccagcg gcacaatcgt tattgattgt tagatctaac gtacctctat gggttccctg
     5221 tcggcaaacg catgtctttg ctgtgcgtta atagttattc tgcttaccag tggctgtatg
     5281 accaatgccg aaaaccggag taactacatt ctggagaaaa tgaatgcgag ccaggaatac
     5341 tacgatgacc tggtcacatc ggaccccggc aatgctacgg catggtgcat tcgaggaatg
     5401 tattatctca attgtaacgg tcaggatgcc gaagctatgg agagctgtaa cagggcgctt
     5461 gaactggacn ncttaaaagg ggtcattctg gtaaatacgt ataagcgcga tgaagcactc
     5521 ttgtgttttg aaaatgcgac gaagtacgat cccgggctga caaaagacgt gctgttcatt
     5581 actggcaata tgtgatgacc tgggtttcta ttgttatcac ttttaagaga cctgtttttt
     5641 acgaggtctg aaccaaaaat tgggctttta agtggccctg ttttgt
//
LOCUS       DAJB01000080            5450 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_90349, whole genome shotgun sequence.
ACCESSION   DAJB01000080 DAJB01000000
VERSION     DAJB01000080.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_90349"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2408..2501
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          977 a         1736 c         1751 g          890 t
ORIGIN      
        1 ttaaccgggg gtagttgact gccatgtcgc cgaggagtat tccatggctc cctatcgccg
       61 tccagtcgat gtggacgtct tttggggcct ccatgccctt ttgctgggtg agcagccagg
      121 tgactgtctc catcgagagg cccagttcgt cggagatctg gccgggagtg tgcccctctg
      181 cctggagcgc ctttgccttc gtgatcaact cttcaagggc ggacatggag aagagatctc
      241 cagggaatct atttaagttt tcttttgatt gctgcgccgc agaccggaca gtctcccggc
      301 tcccgccagt agcgcccgca cccgctgcac cggtagcgcc accggatcgc ccgggcgggc
      361 cgttgccgga tgctccgggt ctcgataccg aggcggtgcg ccacattctg aaccgcgaag
      421 tcgtcggtga cgaggacggc cgagagttcc agcgccagcg cgagaatgtc ccggtcggtc
      481 ccggagagca cgccggtatc cccggtcgcg agggcggctg cttcaacccg cacccggtct
      541 tcctctcgcg gctcccggac ccggaggcct gtcgccgcga gcacctcaaa ccggcacttc
      601 gcgtggaggt cgccaagttc ctccaccacc gacggggtgg tccatgccgg gccttcaacc
      661 ggtatctccg agaagaatat cgaggcgtcg aggacgagcg tcatgccgag aactccacga
      721 tatcgttctc ccggcaccgg atctcatacc cgaattccag aaggagatgg cgggccgtct
      781 tcgcgtgcat cgagagagtg tgggtgctgt actccccgcc gtagcaggcg aggtagacga
      841 ggagctggtc ggcgaggtgg acgtcgaccg tacccgggct gcggcattcc tcgatcaggg
      901 cctgtgcggc cgcccggccc accttctcgg caggaagccc cctcttcccg agagcgacgg
      961 cacccttcgc tccgctccat gcggtgacgg aactcccggt gctcgccccg ccctcccggg
     1021 ggtccagggt cggtgtgcag gagagaccga gnnccgggag gcccgccgag caggagacga
     1081 tgccgcaccc cggctcctcg tccgggatca cgatcgggcc gggccgggcc ggctccaccc
     1141 ggacgcgcac ccgcccgcct ccctgcggat agtaaccccg ctcgagcacc tcgatctcta
     1201 tcgaggcccc ggcgcggcgg agcaccggcg cgaggacgcg gtccaggtag tcgatcgtcg
     1261 ggctccacac cacctcggtc ccgcccgtga ctatgatact cccgccggcg tggagagcga
     1321 ccgggagcca tgcctggagg atgaggggga tgctcccggc ggttccggga tcgatctcca
     1381 ggtcggcccg ccggagtcgg ccgggggtga aggtgatctc actgctccgg agcgaaagcc
     1441 ccgtgcattc ggcatcgcag gctccggcaa ccgcccggac cgcagcgatg tgctggggcg
     1501 cgagcccggg tttgtcccgg ttctgccgga ttcgggtgac ggtgaccgga gttgtggaga
     1561 tcgccgatag agcgaccgcc aggcggagga tctgcccccc tccctccaga tgagatccgt
     1621 caacggtgag catcttccct gatcgcggta gcgattgcgc tcgcggccga tatgaagctt
     1681 gaggcgttct cgtaggtgtc gctggagacg accgaggaga cccccgccgc ccgcaggcgg
     1741 gtataggcgc cgctcgtgag caccccgtga acgcaggccg cgtggatgga tgcggctccc
     1801 tgctcccgga gcatgcaggc ggcggtcgca agggttccgc cggtggagat gatatcgtcc
     1861 acgatcacga cctcccggcc ggctgcatcg atggtctttg gggcgatccg gacctcctca
     1921 cccgagagcc gggtctttac gaggtggtcg cagtcccacc cgctgaccgc cgcaacgtcg
     1981 gccgcaaagt cggtcgcccc ttcgtccgga gcgaggacca gggggttctt caggcgcagg
     2041 tcgccgatgt acccgcctat ggccggggcg atggtgacat ccttcgccgg aacgccgaaa
     2101 tgggccagca cgccgggctc gtggacatcg accacgaacg cccgctcaat gccgggcgag
     2161 agggctcgcg cgatcgcccg ggcgcttatc ggctcgcccg ggaaaaaacg cctgtcctgc
     2221 cgggagtagc cgaggtaagg gatcaccagc gtgttccttg attggtcggc ggcatcgatc
     2281 gcgaggaggg tctgcacgaa catatcatta tccacgacgc tgctgacgat cagggtctca
     2341 tcgtccagtt ctccgcaccg gagatatagt tctccgtcgg gaaaccgggt gaatttcgtc
     2401 tcgaccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagctgag cgcatcttca
     2521 gatatcgacg tacccccgag tctcatcgat caggtcatcg gtcaggaaca tgctgtcgag
     2581 gtgatccgga aggccgcggt ccagcgcagg catgtgatga tgatcgggac ccccggaacc
     2641 gggaaatcta tgctggcgaa ggctatggcc gagctcctcc ccaaggagga actgcaggac
     2701 attctggtct acccgaatcc cgaggataac aataatccca tcatccggac cgtgccggcg
     2761 agccgcggca aacagatcgt caacgcccac aaggtagagg ccagaaagaa gatccagatg
     2821 cggagcactc ttgtcatgct cctcatcata ggtatcatcg gctacgcgat catcacctac
     2881 cagtggctca tgggcatcat cgccgccgca ttcatcttca tggcattgaa gtattcgacg
     2941 ccccgcgaag agacgatggt cccgaagctc ctgatctccc atgacccgaa cacccctgcg
     3001 cccttcatcg acgccaccgg ttcgcacgcg ggcgccctgc tcggcgatgt ccggcacgac
     3061 cccttccaga gcggcggcct cgagacgcct tcccacgacc gcgtcgaggg aggggctatc
     3121 caccgggcgc acgggggcgt gctcttcatc gacgagatca acaccctcac cccgcactcc
     3181 cagcagaatc tgctgactgc gctccaggaa ggcacattcc cgatcaccgg ccagagcgag
     3241 cgctcgagcg gtgcgatggt caggacggag ccggttccct gccggttcgt gatgatcgcg
     3301 gcgggcaaca tcgatgccat ccaggggatg cacccggcac tccggtcgcg tatccggggc
     3361 tacggctatg aggtctacat gcgggagacg atggaggaca ccgcggagaa ccggaagaag
     3421 ttcctccggt tcatcgccca ggaagtcaag aacgacggga agatccccca cttcgaccgc
     3481 agcgccatgg tcgaggtgct ccgcgaagcc cggcgccgct cgaaccggaa aggtcacctg
     3541 accttaaaac tccgtgacat gggcgggctc atccgggtag cgggggacct cgcccggcag
     3601 gaaggtgcgg agttcaccac ggaaaagcat gtcctcgccg caaagtcggc ctcccgctcg
     3661 atcgaggacc agatctccga cgaatacatc cggcggagcc gcgactacga catcaccatc
     3721 gtggagggca cccgggtagg acgggtgaac gggcttgccg tgatggggag cgactccggc
     3781 tcggtgctcc cggtgatggc cgaggtgact ccgagccagg gggcgaccgg cacggtcatc
     3841 gcgaccggta tgctcaagga gatcgccaaa gagtcgatca cgaacgtcag cgccctgata
     3901 aagaagttta ccggcaagga tgtcaggaac atggacatcc acgtccagtt catcggcacc
     3961 tacggcggcg tcgaggggga ttcggcctcc gtgaccgtgg caacagcggt gatcagcgct
     4021 atcgagaaca tcccggtgcg ccaggacgtc gcgatgaccg gttccctctc gatccggggc
     4081 gacgtcctcc cgatcggcgg ggtcacctac aagatcgagg cggctgcgaa ggccgggatc
     4141 aagatggtga tcatcccgcg gtcgaactta gacgacgttc tcatcgagga ccgctaccgg
     4201 gggatgatcg aggttatccc ggtcgaccac atcgaggagg ttctccagaa cgcgctcgtg
     4261 cccgagaacc gcgaaggatt cctctcgaag ctcaagaaga tggcggtcaa cccgaccggc
     4321 atcttcgatc cgaacctcgg gcgctccctg gtctgatgcc ctatggctga accaagatac
     4381 tatgatatac ggtgcgtgcg gggcgagacc actctcgtcg atatcgacaa cggtgtggtc
     4441 gagtccgcag gcacctcatt ttttgacaaa gccgtgatcc gggtgctggg accgaaaggc
     4501 tggggtatcc tcactcgcga ccacgttgac atcgattcaa agcgcgactt aaccgccctc
     4561 atcgaggcgg cagcgcgtct cgctgcggtc acacaggacc agatagacct ggccgatgca
     4621 ccgcgcggcg ctctcccggt cccgcgcctc ggggagaacc cccgggatat cgacctcgag
     4681 gagaagaccc ggctgcttgc cggcatcgag gcggcggcac gggttcctga ggtggcaaac
     4741 acccgggccc gctacaccga ggggatcgac caggtccggt tcctggactc gagcgggaac
     4801 gagttctcgt acgaggcggt ccggtcgggg ttctctgtcc tcgcgattgc gtcccggagc
     4861 ggtatgatgc agatggggag cgaacgcgat catatcatca ccgggttcaa cctccgcgat
     4921 aagcaggacc tcggacggaa ggcgggcgag gtcgcggtct ccctgctcga cgcaaaactc
     4981 gcgaaaggcg gggcgggccg ggccgtgctc gacccggaac tggcgggcgt cttcacccac
     5041 gaggcggtcg gtcacgcgag cgagggcgac ctcgtccgcg aaggcgcatc ggttctcgca
     5101 gggagaatcg gcgagcggat cgggtgcgag ggactggtca tcgtggacga tccctcgctt
     5161 caggagttcg ggttcatccc tgtggacgcc gagggggtgg cggtgcagcg gaccgaagtc
     5221 atccgtgacg gcatcctctc atcctacctc cacaaccggg agaccctcgc ggcagtcggg
     5281 aacggtatcc ccggtcacgc ccgggcggag ctcggggcac cgcccatcgt ccggatgagc
     5341 aacaccttca tcgaaaacgg ggatgcgacc tacgatgaga tcctctctga gtgtaaggac
     5401 ggcatcctgc tgatcggctc aaggggcggc caggtcgacc ccggccgggg
//
LOCUS       DAJB01000081            4539 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_76433, whole genome shotgun sequence.
ACCESSION   DAJB01000081 DAJB01000000
VERSION     DAJB01000081.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_76433"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          762 a         1396 c         1569 g          812 t
ORIGIN      
        1 ggttttaacc gggggtagtt gacgcaggtg ctgtcatatc tccctttccc tcccggaatg
       61 cggcggacga cctggagggc tcgcatgatc tcggcctccc tctccccgtc gggtcccggg
      121 gtgcaggccc gggcagtaaa tgctccccgg cacccctttc cctgcgggtc cggatagaca
      181 ttctgaaata ccagaactcc cccatccatc ctcagagacg gcatgtgcga ctgctacttg
      241 catccctgta caggcgatca gagagcccga tccgggtata gcatcactcc tggcaccggc
      301 gcaccggagt acgaggagcg ggcgggagga cggcggtgac ggcggccctg cggacagcgc
      361 tcgtcctcct cgtcgcagcg ctggcgcttg tggcggggac gggcgggctc gtggtcgata
      421 acgccgacct gagcccggca gaggtgaaac tcctctttgc gatgcgggag tataccctga
      481 cctacaacga gtacgccgac cggctgccgg gatgggtgac cgggccgttc gagggcgaga
      541 aggtcaacct ctatgtccag tctcccgacg gcgtgctggt catcgggatc accttccatg
      601 accgcaagat cgtccagttt gatgcaaagg gctgcccgga cccgatcgtc acggtgacca
      661 ctaattctga gacggtggag gatgtcatga cgtcggatag gccgtttgac acgttccggg
      721 cggcgatgcg gaacggcacg accacgatcg aggggaacaa cctcctcggc acgttgcggt
      781 cggggatggt ccagttcggg ctccgggtga tggacgtcat cgggctatga cggacccaaa
      841 aagtcgttgt tgatgagagg gggcctcaca gccccacgag gcgcatgcag aacccgaagg
      901 tcagccccga catggcggac gtcgccgaga gggccgcgag cacggcggcg tttaccacag
      961 gactggtcct gagcggggtg taggtcgggt tgcgctgcag tcgccgcact atcctgaacg
     1021 gttttgcggt cgttgagatg ggggatttgt aacgtctgat cctcaatgtg aacacctcct
     1081 cctgctgggt ggatactatt tcctgagtgt cgattgtttc ccggaataat aattattgcg
     1141 gtttcgctct ccgggcggag gaggcagtcg cccccggcag gcggccgagg gctatgtttc
     1201 gccggatcag ggatttgggc cacttcctgt accaggctcc ctgagccctg cccgcggcag
     1261 gtttatccgt tcgccagcaa gaggactcat aagatgcatc gatacgcaag gaccctcctc
     1321 tacggcatac tggtgtttgc cgtctatacc ggtatcagtt tcattctcgg cgggcgggtg
     1381 aactggatcc tcgcggcagc gacggccgta ggggtgatga tcgcggagtt cttcgtcgtg
     1441 tcacggcgga ggcggtgagg agatggcggc cggtgtgcgg gcagagtggg agcggctccg
     1501 gagagtggcc gtccaccggc cggggatcga gatgttcttc gggctgctgg agccgcatgc
     1561 cgccctctac gaacgggcgt tcagccgcta caaggcccgc caggagcacg acgacctcga
     1621 acgcactctc cgggaggagt tcggggtccg ggtgctgcgg ctcaaggaga cgatcctcga
     1681 tgccgccgac cgcgacccgg cggtgcgccg gcggctcgtc gattgggcgc acgagaccgt
     1741 ctccctccgg ggcggcagga gagaggtggc ggaggcacgc cggagcatgg agcagaacgc
     1801 caacgccctg gactcgcagc acttcttcac cctcctcctt ttaaatccgg ttatcgaggt
     1861 aggacgggga cacccccgtg gcggggtgga cgtccatatc ctggagcgcc agccgctcgc
     1921 aaacctctac ttcatgcgcg atcagcaggc ggtgaccccc tgcggcctcg tcgtcgcacg
     1981 gccggcaaag cggcaacggg cccgggagcc cgagattacc cggttcctct gggagatcct
     2041 cggcatcccg gtcgtcggga cggtgcagga gccgggaacg ttcgagggtg gggacttcat
     2101 gccgatgggg gagttcgccc tcctcggcac cggagaccgg acgaaccatg ccggggtatg
     2161 ccagtttctc ggctgcgccc cggggttcga cgagatcggc atcgtccacc agccaggcca
     2221 cccgctcatc ccgggcgacc ggcccgaccc gatggtcgat atgcacctcg acacctactt
     2281 caacgtcgcg ggaagcggtg tggtgatcgg atctgaagtg cttctccgga gggcgcgggt
     2341 cgatatctat tgccgggcgg acggggggta tcagtcatcg ggcgagaccg caagcctcta
     2401 cgactacatc cggtcgaagg ggtttgcggt gatcgatctt tcgatcctgg agcagctctc
     2461 ctacgccgca aacatcctct gcatccgcga cggcagcatc cttgcggtgg agggggagcg
     2521 ggtgatgcgg acggtgctcc tgaacctgga gcggaaggcc ggagacgatc cgcaacgcta
     2581 cgggcggctc ctccgccacg cagaggagga ctaccgccgg atcaggaacg aaggacggtt
     2641 cttcccgcac aaggcggagt tctcccggca cgatatcgag gtatacccac tccgcctcga
     2701 gaacctgacg gggggctacg gcggccccca ctgcatgacc tgcacgctgg agcgggggta
     2761 acgggatgcc ggggaaggcc gtggttatcg cgctcggcgg caacgccatc ctgcggcacc
     2821 gggagacggg gacggccgaa gagcagttcg cgaacgtccg gagagcctcg cggcgtatcg
     2881 ccgagatcgc gagcgacggc tacgctgtcg tcatcaccca cgggaacgga ccgcaggtgg
     2941 gtgacatcct cctcaaaaac gagatcgcaa aggagtctct tccaccgatg ccactcgacg
     3001 tctgcggggc ggagagccag gggatgatcg gctacctgct ccagcagtcg atgcatgagg
     3061 cccttctggc ggcggggctc gactgcccgg tcgcgacggt gcttacccag accctggtgg
     3121 acggcgacga cccggcgttt gaaaaccccg aaaaacctat cggcccgctc tacacggcga
     3181 tgcaggcgaa acggctccag gaggagaagg gctggcaggt ggtgcaggtg ccggggcagg
     3241 gttgccggcg agtggtcccc tcgccgcgcc cggtcgcctt tgtcgaaggg caggcgatac
     3301 tccggctctt catggccgga gtgatcgtta tcgccgcggg cggcgggggt gtcccggtgg
     3361 tcgcggagag cgagggcacc ctccggggcg tcgaggcggt ggtggacaag gactacaccg
     3421 cggcgctcct cgcccgcctc atcggcgccg gtgacctgtt gatcctgacc gacgtcgagc
     3481 atgttgcctt aaactacggg cggccgggcc ggcaggatat cagcgagatg acggttgctg
     3541 aagcacgaga gcatctcgcg gcggggcagt tcccgcccgg gagcatgggg ccgaagatcg
     3601 aggcggccat cgggttcctc gaagccggag ggggccgggt aaccatcgca tcgcttgagc
     3661 gggccgccga cgcccttgcc ggacgagcgg ggacccggat ttgcccgtaa cctgcgcccc
     3721 gccccaacac ttatatatca cgttcccgaa ctcccgtcgg tgatggaagg atatggctga
     3781 taacttctgg acgggtgtca tcgtcgggtg gctcgtggga ctcattctcg gcttcctgct
     3841 gcctatcatc gggccgctgg tgggtggatt cgtcgccggc tggatgtccg ggcgcgggat
     3901 cagcaacggc gcaaaggccg ggctgcttgc cggcatcctc ggtgccatcg tcatcgcggt
     3961 gctgctcctc gtcggcggca cggttctcct cggggcgttc gggttcatcg cgggcctcgg
     4021 gacgtcgctc gtcatcattg tggcggcgtt cgtctaccag ggactcctct ccctgatcgg
     4081 cggcgccatc gccggggcga tccgccggta atcgcaggaa caccgggggg cgatcctccc
     4141 cggctcttct caatcaggag gcgaggattg gcagaggacg gcggttcata ccgggtctcc
     4201 acactggtgg gactcatctt tatgctcgtt ataagcccct tcttcccggt aggggcccga
     4261 tcctcggggg gatcatcggc ggccacctcg ggcccccggg agcggtcggt agtgccctac
     4321 gaaacaactg ataatacttt gttaagatcc catccgggtg ctcccgattg attgcaatcc
     4381 ggagcacggc tttccatcgg gctgtgcacc tggcggtgtt cgagctccgt tcctgtcgga
     4441 acgtcgcgta tcgccatgac tgcccccgcc cccggaggcg ggggaggagg ccggaggccg
     4501 ggcggggtgg gggttacagg aaaccttagt atgtggaga
//
LOCUS       DAJB01000082            4727 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_86861, whole genome shotgun sequence.
ACCESSION   DAJB01000082 DAJB01000000
VERSION     DAJB01000082.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_86861"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          899 a         1528 c         1322 g          976 t
ORIGIN      
        1 tccctaaaac ctcctcggtc cggttgttga tgttgttccc gaacgacggc cccttctggg
       61 acttcagttc aaggacggca accagttcct tcctttcgtc aggatagtgt ctgacaacaa
      121 taaggtccca ctgcttctcc gcacggaagt atccccggaa ggtcgagaga gctcttccgc
      181 gtgcagatat cgccctcagg caccccgata tccaccagca gatccaggat gagcgagaag
      241 aaccggtcca tgcgctttcc gcccgtcgct tccgaacgaa agccctggtc tcttacaccc
      301 cggttgttct gatcttttgc ctgcgatgct ctcttatccc agaaataccg caccgcacaa
      361 gaaaaatagt cctctcggga aatttcccgg agtatcctgt cactcaccat gaacctgcct
      421 ctatcagaac aatcgagact gttttgcctg atgagtaaac cgttcaacct ccatgaggaa
      481 atatttttct tctacctcga tgctgatgct gtttcggccc caccgtgcag cagcgaggcc
      541 ggtagtcccc gtaccaccaa aggggtcgag gacggtatcg cccacgaaag agaacatccg
      601 cacaagtctc tcagccagtt ccagggggta gggcgcaggg tgctcccggg tcgatgctcc
      661 cttaaggtcc caaacctggc ggaaccatgc cttctgattc tcctccgaga tgaccgagag
      721 cagccgtgcc tcaagtgtgg gcttccggta accgccggat ttcctctgga agaggatata
      781 ctctatatcg tttttgacga tcccgttggg ctcatagggt ttgcccagga acgatcccgg
      841 cgagtctgcc tcaaagcagg cattggctat cttgtaccag atgaccgggg ccaggttgtc
      901 gaacccgatc ttccggcagt gttcctgaat gctggcgtgg agaggcatga cgagatgacg
      961 cccgacctcc cgtcggggta agcagacgtc accgacaacg atgaccagac ggcctccggg
     1021 gacgagaacc cggtaaatgt gtttccagac ccggtcgagt tcttctaaga aggcctcata
     1081 gtcctcaatc tgcccgagtt gttcgtcctt ctcaggatat ctcttcaggg tccagtaggg
     1141 aggcgatgtc acaaccagat gaacgctctc atcagggaga aactcaaggt tgcggctatc
     1201 tgcgtttata aaacggtggt atgtcggcag attaccggat atggcttcaa tctcttttat
     1261 cagcgaggga ttcttggcta tcatcgggat agcacgttga cctcctgcct ccgccagttc
     1321 aagaatctca gcaggcacat ggttctccag cggctgatcc tgcccggttt tattgataaa
     1381 gaacgatctc tcggcaacga tggggagcgt tcccggcgga tgaaacggtc tggaactcat
     1441 gccactgctt ctatgattaa tatcccttat caaataattg taccggagta ttctcctcgc
     1501 gggtttcacc ccgggttctt cgtgcaggtc ggcacccagg tggagaagcc cttcggccgt
     1561 ctccccggca gagagggtcc acccggtcgt cgcaacgatg gcgaggcggg tgccacccgt
     1621 gaactacccc gcgctcatcg gtgctgagat ctccgggcat cgtttccctc cgcattgaga
     1681 gcgggttcac gggttcgatg acgcactcca tacttatgct gccatatcag cccgcttcta
     1741 atatcaagat gcgccgtgtg gagcgtgaaa atgaacccgc gttagtagag cttccgggtc
     1801 tcccgaatct ccttcttgtc gcggaagtgg aatcgagagg tgttctcatt aatccgcctc
     1861 gttttagcac attcctccat taaataggca taccttaacg aggaacggac ctattcacaa
     1921 cagggtagta tcttcaattc ccgatacgct tcaaaaaaag accaggggta tcatccccgg
     1981 cgttcaggcg tcaatccgtt cgagttccgg gggggcgcct gcggaccaga cgctgtactg
     2041 ggcattcggc acccaccgac aggcttcgcc atcggcctcc accgaccaga aggtatagtg
     2101 cgcggtcaac cggtcgccgg caccgttcag ttccaccgac ccgaacagag gcccgccggc
     2161 cccccctgca gttccctcaa gggcgatctt caacttcgag atctccgtgg tgtcggtctg
     2221 cgtccgggtc atggcgacgg tccagagggc atcgtaggat gcgaggctgt agccgtcggg
     2281 ctgccgtccg agaatctccc ggatcttctg gatggtagcc gcacccgacg cagcgcggtc
     2341 ctgctgcccg aactccgggc cggtaaatct cgtctgcgcc gcaaaccggg cggcatctcc
     2401 tgtcacgagg gaatcgagga ggacgttgcc gtcgcacccg taccaccgga ccttcgtgag
     2461 gttctgggtc ttcgcagcag cctccatgat cgccgctgcc tcgtcgaggc tgatcaggta
     2521 gacgccgacc ttttcctttc cgtgcgtagc gatcgcccgg ccaacctgca catcaagatc
     2581 cgccaccatc ccggcatagt cctcctcgcc cggcgcatac cggaccccgt cgaggaccgt
     2641 gccgttgccc cgcttgaacg acgccgtggc gagtttctcc agttcatcgc cccagacgtc
     2701 gccgcgccat gccggcacga tcgcgctcgt cccttcctcc ttgaggtagt atgccattgc
     2761 atcggcctgg taggtgtccg gcggcacgaa ccggtagacg ttgtcgtccg caatcgcaag
     2821 cgacggcgcc gtgctcatgg tgctgatgag gacgatcccg tgttcgtcgg cgtaagtccg
     2881 ggtagcgttc aactcggcgc tggaaccggg cccgatgacg atcctgacac cctgttcacc
     2941 gagcgcctgc aacttctcaa gcgcaacggc ggggtcggtc ccggtatcct cgacgatcac
     3001 cccgacacgg taatccgagt cgatcgccgt gaagtagtcg ttgatatccc cggcggccac
     3061 ctcgagggcg acccggctcg cctctccgcc ttccgcatag tcgccggtga gcggcaggag
     3121 ggcgccgacg aagattccct gtactgcctc gcccatttct gcgggagcag tctgtgttcc
     3181 nntgaaaaca ggtgagatcc ggccgagacc ggccgggaag gcgactggcc gaaggtgcga
     3241 agcctcacag ctcctcctgg tctcccttac tgatcatgtc ccgcgacttc ctctgcatct
     3301 ccatgccgat atcggtatcg atgctcttta ccgcctcctc tgcggtccgg atcacgtccc
     3361 ggcccgggat ctccccgaga atatcctcag acggcagttc cgtgccttcg ggtacctccg
     3421 gggcctcggt cggggcgccg aggtactcgg cggcctgctt gataatgctc gagagctcga
     3481 acgggaagat gatcttcgtc gcctgaccat cggccatcct ctttaaggcg tcgagcgaga
     3541 ggaccgtgat cgcccgccgg tcaagcggcc gggcgccgac cgagaggatg cgcaggccct
     3601 gtgcctcgcc ctgtgcctgc aggatcctgg ataaccgttc accctcggct cgcaggatct
     3661 tgctctgccg ctcgccctcg gcctccagga tgatgctctg ccggttcccc tccgcattga
     3721 ggatcgctgc ccgcttctca ccgtccgccc ggaggatcgc ggcacgccgc tcccgctcgg
     3781 ccgcggtctg ttcggtcatc gcctgcttga ccgcaccgac cgggtcgacc tccttgatct
     3841 ccacccgctc gaccttcaca ccccaggcgt cggtctcacg gtccaggata tccctgaggc
     3901 gggcgttgat gatgtcccgg ttgtagagga cctcatcgag ctccatgtcg ccgatgatcc
     3961 cgcgcaggct cgtctgcgcc agcgccagcg tcgccgtccg gtaactcatc acctcaaaat
     4021 aggccttctc ggggtcgatc acccggacgt agacgatcgc gtccacgttc gtcggggagt
     4081 tgtccttcgt gatcacctcc tgccgcggaa cgtccatcac ctccgtccgg aggtcgagtt
     4141 tcttgaccac cgtgatcagc gggaccaccc acctaaagcc ggggttcatc ctcccgatgt
     4201 actgcccgag acgtatctgg agtccctgct cgtaaggctg cacgatcacc acgccacggg
     4261 cgaatatgat gacgatgacg atgatgagaa agaccgtaat caatccggtc cacggaatct
     4321 gtccagtaag aagcatctca caaaacctcc ttcacgacga cgtgcacccc ttcggagcgc
     4381 acgacgataa tccttctccc ggccgggatg ctcccggact cggaacgcgc gctccactcc
     4441 atgctcccaa gccgcacctt ccccgcgagc gtctccggat cgaccggcat gatcaccgtc
     4501 ccctcgaggc cgatgagcga gtcccgggag agggtcgtcg gcggcccccg accgggcgtg
     4561 agacggccat agagccagac tgtgatggag gccgcaatga tcgccgccag aacaccgacg
     4621 atgagaccgg tgggagaact gagaatatcg acgccgagga ggaggagaac cccgaggatg
     4681 atcaggactg tcgcagggac agcgatgaag aacccgggct ggtagac
//
LOCUS       DAJB01000083            3169 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_142509, whole genome shotgun sequence.
ACCESSION   DAJB01000083 DAJB01000000
VERSION     DAJB01000083.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_142509"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          553 a          921 c         1040 g          655 t
ORIGIN      
        1 ttcgctcact cttgtcctct ctgagaatct gcttgaatct tgtgatcttg ttctcaatgt
       61 atgccttacc acatatggcg atcacttcat cggtcttgaa cataaagggt ctttctacct
      121 ctagagaact caagataaca actgctctct gactgtcttc atcggtgaga caagtttcac
      181 gcgccaattg cttatgccga gctttgatac cttcgaccat gttctccggc gatacaataa
      241 acgactgcgg ccaatattcc atcggcgtat cgcgcatata ttccaaaatt ggtttaaggg
      301 gtgcttcagc ttcagagtgg gttggcattc tcattcctcc atacacctat ttttctcatt
      361 tttgttggta ttaatcttgg ccgatacggc ggggactggt agcccgtgga cctgcgtcgg
      421 gcgctcccgc catagcagaa ctgattcgta gtggtcactg gataggtcca cgcaagtgcc
      481 ccagacggcc atcagactga cctccagaca tgccaccggc gacggccctg ctcgacccgg
      541 acggcagacc cttcgtgcca ggcctccttc gctgcagcgg ctgcttgcgc ttcagtccgg
      601 tagccgatga gggtcctgcg gaagaggcgg ccgtcctcac gccggtagat catgcggtcg
      661 cctcctcggc agcgctccgg acggggaaga cggagctctc cggggggtgc ccgaaaaagt
      721 ggtggtcgcc gatcttgcat gtcacccaga ccgaatagag aaacgtcggc aggttgttgg
      781 cgcacgggcg ggttccgaac accgcgttgg aggggaggta agggaggatg gagtatttca
      841 tgcctgcacc tccggttcga tgcctacctg gtagagcacc cagtccttga tggccacggg
      901 gctgctggtg tccaccccct cgtattcgag gagggttagg aggcgctccc tctcatccgg
      961 ggtcaggacg atgagaagat gcctgtccaa ttcggtcata gcagatcgcc ccgctgcagc
     1021 agctcgccgg ccttgacgtc ccgcgtgcat gtccgggtct cggtccggcg ggatccccag
     1081 atgtccgccc gcatcgccga gatcatgacg tcggtcaccc ggaggagggc ggcttcctct
     1141 ttggggatgg cgtcggatcc caggacatcg atctcctggt cgtctccgac tctctgctcg
     1201 accgctgccc gggcctcctc gaccaggtcc tcgcggacca ccagcaggta ctcgggccag
     1261 acttcggtct cttccaggta gcggacggcc ttgagataat acggcgagga cgtggacgac
     1321 ctgtccaccc ggaggatgcc gtcgggcccg acgaggcggg tggccacccg aagaggttca
     1381 ccgctcatcg tcttcatcct cctctcgcag gacctcgacg ccgtcgatga gccacatcat
     1441 gcgatcaccg cccgggcgaa acagaagatc aggagcatgg tctcgatgac gatgccgaag
     1501 agcatcccgc agatgaacca caggaacttc atggcgtcgt cctcctgcgg gtgttgctga
     1561 gccccaggca ggccgtgagc cagtagaagc cgaacatgat gacaacgtac gggttctgcg
     1621 tcaggaggcc ccagctcacc cagagggcgt tgccgaggat ccagagaatg aacccgaacc
     1681 gccgggactt aagcgagtag ccggagacga agagggcccc gacgatgccg aggatgacgg
     1741 ccggagcctg tagcaggtcc atcagggctg cacctccagg agtttcctgc cgggtttctg
     1801 cctggccggg agcggcacca tgggggtccc ctgcaggcca cttacggtga tagcgaaccg
     1861 gtgccaggtg ccggtcatgc tgccgggttg agggtcgacg tagatccaga gttctacttt
     1921 ggtcgagggt gggtggggga cggcctccat ccgtacgacg tccttccggt tcgttctgta
     1981 gacagactct attgacttga tgggggtctt cgtccgcttc acctggatca agagggtctc
     2041 ttctgcgttg ctggcgatta tgtcgaacgt gcccctcgag gcgtggttgc gctctgtcca
     2101 gtacgatagc gcctgcagga tcttgactgc cttgagctcc gcccggtaac cccgctcata
     2161 gagcttattc gacatgggtc tcctccgggg aggggatgaa cgtgccgtat tcgtgcaggg
     2221 cggcgcaggc tcggtcgacg tcctcttcgg gaacttggac gatgtagtgt gtggatctgc
     2281 cggggtcggg ggtgagcgta ggggagatac cggtaaactc gagggccctg tcgacgaccc
     2341 ggacgaagtc ccggtctatg gtacagaggg ggacaagggg tgtcatgcct gctctcccgc
     2401 ctgcttccac cagggctcaa actttagcag atcctcgggg tctgggaagg tcccggtgaa
     2461 gcagagcgcc cggtcgtcca gggtgacgtg tgcagggggt ttttcggcgg agattttgac
     2521 tggtgggaac ccgttgtccc ggagccagac gcggatggcg gcgcggccga tctgagtcct
     2581 ggcccgggtg gagtggatga cgacgtcgaa gttctccatg gcggcctgga caaactcgac
     2641 ggcaccgggg accgagatgc cgtcgataat ggcatcgccc cgataccggg tgtactggtg
     2701 cagcacgccg tcgaagtcga gggcgagggt cggcttcatg acgaggcctc catgttctcc
     2761 ttcacagcct tcgtcagctc gcgcatctct tcctgaatgc ctgccagggc agcggcacat
     2821 gtccggatcg cgtccgggag gtagatgagg agctcgaccg ggttgacgcc gtcctcgagc
     2881 ggggcgagct cgaagagggt ccgggatggg ggtgctgccg ccagcgaggc ggtcaggtcc
     2941 tcaaaggtca tgctgatcgg gacggcgccg gtccgctgca gctcgaccca gaggtcttcc
     3001 ggggtccttt ggggtgccgg ggggaggagg aggctgctga tctcctcggg ggtgaggtcg
     3061 gagcgctgca ggaggcggat agcctgcatg gcccgtttcc gggtcacggg accgggggga
     3121 cggccgcctt ttttcttctg ttgtggaggc tcgggggagg cgatcatgc
//
LOCUS       DAJB01000084            9424 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_49165, whole genome shotgun sequence.
ACCESSION   DAJB01000084 DAJB01000000
VERSION     DAJB01000084.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_49165"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    7772..7822
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8120..8190
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1753 a         2996 c         3074 g         1477 t
ORIGIN      
        1 ccccgcgagc cccgcggaga taaatacccg aatacggcag atatccctca ctgaggcgaa
       61 gcgcagtgag aaacgaactt gagagcgaga tcgcgagact ctcctccctc gcccgggagc
      121 gcggcgcgga agcgcggcgg atagcggccc acgacgtcgt cgtcgcggag tgggtacggt
      181 tcaagtgccg gttcgggtgc aagggctacg ggaaacacat gtcctgcccg ccgtatgccc
      241 ccgctcctgc cgatacccgg cggctcctcg ccgagtacag caccgccctc ctcctccggt
      301 tcgagggcgt gccggggcac cccgacctga aacccgaaga gataccgctc gacttccacc
      361 cgttcttccg cgacctcgtc ctctgggtga actcgacggt ccacctcctc gaaaagaccg
      421 ccttctacga cgattaccca aaagcgttcg ggttcggcgg atatccctgc atatactgcg
      481 agcaccggca ctgcgtcgcc gaagagcacg aaggggttgt cgacgagagc atccgcaggc
      541 tgtgtcggca catggacctc gtccgcccga cgatggaggg tgccgggatc gacgtctttt
      601 ccaccgcccg gaaggtcggg tgggacctgc acgtcgtccc ctgcagggac ctcgagtatg
      661 gaaaaatcga gcacggcaac atcacctcga tagggctcgt tctcatcgag tgacccgccg
      721 gccctcagtc gtcgcgcagg ccccggatga actcatcggt catgcgggcg gcgtcgtcgt
      781 ccctgacctg caccggcggg tgcttcatga agtaagcgga tggggatacg agcgcaccgc
      841 cgatgccccg gtcgagggct accctgcagc accggatggc gtcgatgacg atccctgcgg
      901 agttgggcga gtcctcgacg gagaggcgga ggtcgatctg catggggacg tccccaaaga
      961 gcctcccctc catcctgagg aagcagacct tgttgtcctt ctgccagcgg acgtagtcgc
     1021 tcgggccgat gtggatatcg tcgtcatcga gaggttcttt gaggacagac tgcaccgctt
     1081 cggtcttcga taccctcttc gaggcgaggc ggtcacggtt gagcatattc aaaaagtccg
     1141 tgttgccgcc ggtgttcaac tggtaggtcc ggtccagttt tactccccgt tgctggaaga
     1201 ggttggcgag gacccggtgg gtgatcgttg cgccgagctg ggccttgacg tcgtcgccga
     1261 tgatcggaag gccgcgttcg cgaaacttcg ctgcccatgc cgggtcgctc gcgatgaaga
     1321 cgggcatatt gttgacgagg ccgatgcccg cctccagcgc acacccggca tagaaccgtg
     1381 cggcctcttc ggaccctacg gggaggtagt tgaggagcat ctcggcaccc gactcctcga
     1441 gcgccaggac gatctcctcg cgtgacgcct ccttctcgtc ggcgaggacg aatctgtact
     1501 tttctttatg ggcctgcatg tgctcgggaa agccgtccag cacccgcccc atccggacgg
     1561 ccacgccggt ccttggcata tcggggcaga aggccgtggt gcagttcggg ggggcaaaaa
     1621 tggcttccga gacgtccttt cctaccttcc tcgcatcgat atcgaacgct gcgaccactt
     1681 cgatgtcgga tggccggtag ccgcagaggt cccagtgcat cagcccggtt gcgtcctcct
     1741 cgctcttccc ccggtagtat tcgatcccct gaagcagcga actggcacag tttccgacac
     1801 ccacgattgc aattctgatc gagtccacga cagcctccca aatgtctggt atgctgctta
     1861 acgcgcccat ctctccgggc ggatgcggta agcatgggtt gagaagacac aactgtggag
     1921 acagttgcat tttgactata atacggagcg ggaaatataa gggtatggtt tgggaagcgt
     1981 cggaggttcc atgccgcagc agagggtgga gtgccgggca ccctctgcag gttagaaggg
     2041 ctgcaggggc gcctgaccgt aagcgcatcc cgggggtgaa cacgccctca tccgcgagag
     2101 ggtcgtcact gtgcgagaaa tcctggaaaa agaacgcacc agacctggtg gacagatgcg
     2161 ccgaccggga ctcgaacccg ggtttaggcg ttggcaacgc ctagtgataa ccactacact
     2221 atcggcgcac gcttgtgttc tcacacctgt gtgccttaca atgtggcact tgctggctaa
     2281 taagcctttc gaatcacggt cctgaccctg tggtgccgtc cccgcaggtt cgacggatcc
     2341 ggtcggagtc tttgaggtag gagcgtcttc gaaacggcga tgcgatcgcc gccgcgcacc
     2401 taccggcgtc cgtggtcgag cgcactccgg atattccggt tcgcaagtat ccagagcccc
     2461 ggcgtcagga ccaggatgaa gatggccatg gcgtaggcaa cgatctcagc ggagaagagc
     2521 aggaagttga agatgccgtg cagcaccatg gcgagagcga gcccccggaa gatgatcccc
     2581 gcccgccgct cgggggccgc gaacttggcc cggccgaggg catagcccca tacgctggag
     2641 aagagcgcgt gcccggggac ggagaagatc gcccggacga cgatcgtgcc gagggcaagc
     2701 gacggcgacg tcacgtatgc cgtaaagaca tagaggacgt tttcgagcga tgcaagcccg
     2761 agggcggcgg cagcggcgta gacgatgccg tccatcggtt cgtcgaactc ggcgttccgg
     2821 taggcgaacc ggcgcacgac cgcgaacttg ccgtactcct cgacgatcgg cgcgacaaca
     2881 gcgcccatga tcagcggtga taagacaaag aggccgaaga atccctcgac gaaggccacg
     2941 ggaaacgtga cgaggacgcc gaggaggaag agtttcacaa tcagggccgc cggctcgggg
     3001 tcgaatttat ccctgcggta gaaataccac gcccagaata tccccggtgc cagcgccagg
     3061 gcaaggatca ccagcggctc accgtcaacc atgttgatcg tgattcatgg ttgcataccg
     3121 gaagagcctt tctcttcaac aggctgcctc gcaaggtagc ccctagcagg atcgcaaacg
     3181 ctgtttcccg gtcaaggacc agaaaggggt tactcgggga ggaggcggta ctccggggcg
     3241 agcccctcga gcaccagcag cccgtagtac cggaagaacg tgacgaacgg caccgcaatc
     3301 aggagcgcga ccgggatggc gatgaggagg accaggagca gcacgtagtt cgcctggact
     3361 gcggcgagga ggacgatccc gatgaggatg aacggtatcg ctatcaccac cagcgcaagg
     3421 atgaccagga tcgtctgcac gatcgcggca atgaggccca gcacgagccc ggcgaggacg
     3481 tagacgacgg tctgccagaa cccggccgat attgccccga taagcctccg ccagccctcg
     3541 atgacgccgc agtcctcatg gatcatgatg gggacgacga agtcggtggt caggaggaag
     3601 acgactccaa agagcagggc catgaccagg atgaccggga tgagcgcgag gagcaggagc
     3661 ggcgcggcaa cgccgacacc cgcccccgaa aggccaaaga gcatgaaggc gagcgcagca
     3721 acgccgagga ccaggatcag cgtgagggcg acctggaaga ggaagaggcg cactcctttt
     3781 ccaaaccgct caccgaggaa gcgtctgata tggatatctc cggtccggag catatcgacg
     3841 aagacgaact gcatcgccgc gccgacgagc atccagagga gggcgaccgc cagcacgacg
     3901 acgatgaacg ccacgatcag cggcgcgatc tcggggaggg actccgcagc gccgggcggg
     3961 aggtcgcccc ccgtagtcgt attggaagac attcgggaag cttgcgcccc ctcccacaaa
     4021 gagcgcgatc acggccaggc ggagccagat gccccactga ggtggccaga gcaggctttt
     4081 cgtgcgctgg aaggcgcctt ccagcctgct gaacgcatac atctcttcag ccatatcagt
     4141 cacggagaaa agggcgtttc tgccattaaa accttctttg gacgaaccgg ttccagtatc
     4201 ccggcttccg ggcatatgcc cgcgggtttt cggggaatca ggtttttcaa caccttaatg
     4261 tagtccgttc accacactat agaacgaatg atccctctca tgcttgacct gacgggcagg
     4321 agggtgctca tcttcggcgg aggcgatgtc ggtgcccgga aggctgcgta ctttgtgcat
     4381 gaggcggagg tgacggtgat cagccgttct ttctcgccgg accttgaggg cctcgcgatt
     4441 caccggcagg aagccgacct ctcggccctc ccggacgagg cgctccggga cctcctcgag
     4501 ggtgcgttcc tcgcggtagc cgcgacaccg gacccggaac tcaacgaccg tatcgggaga
     4561 gtatgcgccg gaaccggcgt cctcttcaac aacgccgcag gcgttcccgg ggatgtcatc
     4621 gtcccttcgg tcgtccgggg cagccgctac ctggtcgcga tcagcacccg cgggaagagc
     4681 cccgccgttt cgcggtacct ccgcatgagg ctggaggcgg attacgcgga cctcgacagg
     4741 atgatcgacc tgcaggagga gatgcgctcg atgctccagg agaccgagcc ggcgcaggca
     4801 gagcggtccc ggatcctctg gagcatcctt gcggacgaag agatccgggc ggcgcttgcc
     4861 tccgactacg accgggcacg cggactggcg atcgagaggt acctgcatgc ctgagtttgt
     4921 cacgacaccg ctcgcgctcg cgggcatgaa ccaccacacc gcggacatcg ccacgctcga
     4981 ggcgttccgg ttccctgacg aggcggcgtt cctctgcgag gcacgggagc ggttcagggg
     5041 cgtcctcctg ctgcagacct gcaaccgcgt cgaggtcctg gtgcagggcg acgcacggag
     5101 cctggaagcg ttcctgcagg agcagggcag acacgacttc atggtcatcg aaggcgaggc
     5161 cgtgccccgc cacctcctgg aactggccgc aggcatcgac tccctggtcg tcggcgagga
     5221 ccagatcctc gggcaactca aacgagcgct cgcgaccgcg gaggaggcgg ggaccgcgaa
     5281 cagcgtcatc gggctctgca tcaacaaggc ggtgcacgtg ggggtcagga tccggcgcca
     5341 gacgcagatc aaccgggggg cggtctccgt cggctctgcg gccgtgacgc ttgcagaaaa
     5401 actcctcggc accctgcgcg accggcacat cctggtcgtc gggagcgggg agatgggcgt
     5461 tctggtggcg caggcgctcg ccgccagggg tctcacggcc atctacgtcg ccaacaggac
     5521 ctacgagcgc gcggtgatgc ttgcggataa gatcggggga cgcgcggtca atttcaagga
     5581 cctctaccgc tacatcgcgc tctccgacgt cgtcatctcc tgcaccgccg cgccgcaccc
     5641 ggtcatccgg accggggata tccgggaggt gatggaggag cgcctctggc cgctcgaccg
     5701 gcacccccgc cacctggtcc tcatcgacat cgcccagccc cgcgacgtcg aggagggggt
     5761 gcggtcgatc gaaggcgtgc acctcttcac catcgacgac ctcagaatcg tcaacgacgc
     5821 tgctatggac tcccggagga gcgaggtgga tcgggcacgg gggatcatcg acgaggaact
     5881 cgaccacttc atccggctcc tccggcggac ggcggccgac gagacgctcg ccctcctcta
     5941 cacctgggcg gagtcgatcc gggcgcgtga acgcgaccgg gccctcgccc gcctgggtgg
     6001 gggggacggc cgcacggcag aggtcgtcga cgacctgacg cgggcgctca ccaaaaagct
     6061 cctctccgac gcgacgacag ccatacgcgc gagcgcggag tgcggcgata tagcgaaagc
     6121 agaggccctg atgagggcga ttacccgggg agaacaatgt ttccagaacg acgaatgaga
     6181 cggatacggc ggcggatcat ccagccgctc ctccgtgaaa cagaactcag aaagaccgac
     6241 ctgatagcgc cggtcttcct ggacgaatcg atcagcacac cgctacccat cgcctcgatg
     6301 cccgggcagt tccgccaccc ggtggacggc gtcgccgact actgcgagcg cctcggaaac
     6361 gccggtatca gtgcggtcct cctctttggg gtcccgggca caaaagacag cgaggcaacc
     6421 gaagcctacg cggctgacgg tgtcgtccag cgggccgtcc gaaagatcaa ggagcgattg
     6481 gcgcagatgg tggtcatgac cgacgtctgc gcctgcgagt acaccgacca cggccactgc
     6541 ggcattgttg gagaaacggt cgacggcccc gacctcctca atgacccgtc gcttgcgctg
     6601 atggcgcaaa tagccgtctc ccacgcacag agcggtgccg acatcgtcgc gccgtcgtgc
     6661 atgctcgacg ggatggtgca ggcgatccgg caggcgctcg acgccgccgg ctaccaggac
     6721 gtcctgatcg tctcctactc ctcgaagttc gcgagcgctc tctacgggcc gttccgtgac
     6781 gctgcggact cggggttcgc ctttggggac cggacgacct accagatcaa cccgggcaac
     6841 gcgcgggagg cggtgatgga gtcggaactg gatgctgccg aaggagcgga tatcctgatg
     6901 gtcaagccgg ccggnngcct accaggtcag cggcgagtac gcgatgatca aggcggccgc
     6961 agagcgcggg tggcttgacg agcgggctgt cgccctcgag agcctcaccg ccatcaagcg
     7021 cgccggggcc gacctgatca tcacttactt tgcagaagac gcggcgaggt ggcttgatga
     7081 agagcagtga cctctttgcc cgcgcacaga ccctgatgcc gggcggcgtc agcagccccg
     7141 tccgggcgat caagccctac ccgttctacg tggagcacgc gtcgggctcg caccttacga
     7201 ccgtcgacgg tgccgatctc atcgattgct gccttggcta cggccccctc atcctcggcc
     7261 acgcccatcc ggctatccgg gaggcgatcg agcgccagct tgagaagggg tggctctacg
     7321 gcacgccgtc gccgctcgag atcgaccttg ctgaaatcat caccggcgac catcccgcag
     7381 tcgacatggt ccggttcgtc tcgtcgggct cggaggcgac gatggccgcg atccggctcg
     7441 cccgcggcta cacggggaag caggacatca tcaaggtcga aggagggttc cacggcgccc
     7501 atgacgcggt tctcgtcaag gccgggtcgg gggcgacgac cctcggggtt cccgactccg
     7561 caggcgtcct cgcggacctc gtggcgcaca cccggcaggt cccgtacaac gaccccgagg
     7621 cactggaaac gcttcttgcc gggaatgacg acgtcgcggc gttcatcatc gagccgatca
     7681 tggggaacat cggcccggtc ctccccgacg acggctacct cgccgacgtc cgggagatca
     7741 ccgcagccta cgacgtcctc ctcatcctcg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nngcgatatc atggaactga tcgccccctc cggcccggtc
     7861 taccaggcag gcacattcag cggcaacccg gcgagcctcg ccgccgggtc tgcgaccctc
     7921 cgccacctcc acgagcaccc ggagatctac cggcggctcg acgacgccac gcgggcgatc
     7981 ggggaggtcg ccgcggatgc agggaagggc tcgtttgtca ggataggctc gctcttcaag
     8041 cacttcttcc gggccgcgcc gccgcagaac taccgggagg tcaaggagtg cgacacagag
     8101 gcgttctcgc ggttctggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaggtggtcg gcatgctcgc cgaccggggt
     8221 atcgaggcgg aggtcgtcac gatcgcgacc gagggcgaca ccgcgacgaa cgtcccgctc
     8281 cacgccatcg gcgggcaggg ggtcttcgtg cgggcgctcg acgatgcgat cctccgcggc
     8341 gagatcgacg ccgcggtgca cagcatgaag gacatcccgg cagcgcggcc ggcagggctc
     8401 acctgctgcg cggtcctcga acgggactct cctgccgact tccttgcgca cgagggcccc
     8461 ctcgccgcgg tccgcgtcat cgggtcctca agcacccggc gccgcgccca gctcctccgc
     8521 gacgacccgt cgctagcggt ggagcagctc cggggaaacg tcgatacccg gatacggaag
     8581 cttcgcgagg ggcagtacga tgccatcgtg ctcgcggagg cgggcctcca gcggctcggg
     8641 ctcacgctgc cgggcgagcc cctccccacg gacaggttcg ttccgtcccc aaaccagggc
     8701 accgtcgcgg tcgtctgccg ggacgacccg gccgtcgccg gggccttcgc ggcgctcgac
     8761 catgccccca cccgcctcga cgtggagatc gaacgcgccg tcatggagga ggtcggcggc
     8821 gggtgcttca ccccgcaggg gatctactgc cgcgacggga acctgatcgc cgaggtgctg
     8881 gcgctcgacg gctcccggtg ggagcggatc gagcggcacg tcgcgaccgt cggcgaagcc
     8941 cgggggtgcg gacgtgcgct gcgcaggcag gcggccgacc tgatccggga ggcctatacg
     9001 gctctcgggg tgaaaccatg actggaaaag cgtatcttgt ggggtcaggc ccgggcgggc
     9061 tcggcctcct gacgataagg gcccgcgagg tgatcgacag agcggatgtc gtcctctacg
     9121 accagctccc gggcgaggag atcctcgcga cgctcccgcg ggacgccgag cggatcgatt
     9181 gcggaaagta cggcggcagc cacaccctgg agcagcacga gatcgaggcg ctgatggtcg
     9241 agagggtgaa ggcgggtaaa gtggtcgtgc gcctgaaagg gggcgacccc ttcctcttcg
     9301 gccgcggcgg agaggagatc gagacgctca gggagcacgg catcagggtc gaggtggtgc
     9361 cgggggtcac gagcgccatc gccgtcccgg agatggtcgg catcccggtc acccaccgga
     9421 agta
//
LOCUS       DAJB01000085            6865 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_32811, whole genome shotgun sequence.
ACCESSION   DAJB01000085 DAJB01000000
VERSION     DAJB01000085.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_32811"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1232 a         2134 c         2190 g         1309 t
ORIGIN      
        1 gggcatcctc gatcccgagc atcttcggga tgtccgggcc gtggatatcg aggtcgatga
       61 gacccgtgtt aaatccgcgg ttggcaagag cgtaggcgag gtttgccgag accgtgctct
      121 tcccgacccc gccctttccg gagaggacga ggacgacgtg ctttacgttg acgttggcct
      181 tcggggggag acctttcggg gcgtccgcat ttctcggatc gtcgcacttg gtcgtagccg
      241 agcagccggc gcagttgcct gtgcatgtat ctttatcagg ttcgttcgtt tgagtcataa
      301 gaaatagcct ctgcggtata gtatccttct agagaaggat ctaaatctac tatatattaa
      361 cggtaaatgc cacaaaaagg tatagatgtt ggcatataat cccgttctac tgcccgggga
      421 ccgtttcatg ctcggatcat cctccaccgg cgggggggaa gagggcgacg gtatcgccat
      481 cgttgagaga tgtttcaaga tccccgctaa agtggatgtt cctgccgttc tttaagatgt
      541 tgacgtaagc gctgaccacg cccgtggagg tgaagagggc gtcgccgagc ccggggtatc
      601 gggcgacgag gagcgtgagc aggtcgccga ccgtcgatcc gccggggacc tccgcttcat
      661 gctctgccgg catgatatcc cggagcatcg caaacgccct gaccttcacc gttatggtct
      721 cctcgccgcg cgtgtcccgc ggaaccgcct tcacctcccg cacatccgac gtatgggggt
      781 gcccggcctt atcggtatcg tttcaggggg tatggataga tcttgtttat tttcctttta
      841 ggccaggtta atcatttcct ccatccagca gccttattgc cctcgggtga cgcctgtagg
      901 catggtgata acctatgaac ggctacgcag agaggatcct ccacgtcgat ctcgcgagag
      961 agcagacgat ggaggagccg tttcccgaag agtggagacg gacatacatc ggaggcagag
     1021 ggcttggagt ccgtatcctc caggaactgg ttaacccggg catcgatccc ctcggtccgg
     1081 agaacgtgct ggtcttcgcc actggaccga ttgccgggag cggtctcccc atggggtccc
     1141 ggtacgacgt cgtcacgaag tccccgctga ccgggacact gaccagcgcg aactccggcg
     1201 ggaagttcgg caccagcatg aaacgcgccg ggtacgatgc ggtcgtgatc cgggggcggg
     1261 cggagaggcc cgtctacctc ctccttgacg acgggcatgc agagatccgg gatgcctcag
     1321 aactgtgggg gttgacgacg agcgagacaa cctccgctat ccagaaggat ctcggcgacc
     1381 ccggcctcag cgtggccagt atcggccctg ccggcgagcg cctcgtcagg ttcgccggga
     1441 tcatcaacga gacctcccgg gccgccggcc gcgggggggt cggcgccgtg atggggtcga
     1501 agaacttaaa agcggtcgcc gcccggggga gcggccggat caccgttgcc gaccgtgaca
     1561 ggttcctcgt cctcaaaaga gaggttgcgg agaagatccg ggagaacgcc atctccggcg
     1621 gagggctgcc ccggttcggg acgggggtcc tcgtgaacat catcaacgag aactatattc
     1681 ttccggtcca aaacttccag agcgcacact tccctgctgc ggagaacgtc tcgggggagc
     1741 ggatagccga taccatcctc accggaaaga tggggtgcca ggcctgcgtc atccagtgcg
     1801 gccgcgaggt tgaggtcggg gggaagcaga cggcaggccc ggagtacgag acgatctggg
     1861 cgttcggtcc tgactgcgga atcgacaacc ttgccgccat tgtggaggcg aacaacctct
     1921 ctaacgacct cggcctcgac acgatctcca ccgggtcgac gatcgcctgc gccatggaac
     1981 tctcgcagag gggctacatc gacgaggaga tacggtttgg ggatgcggag cagatgctcg
     2041 acctcgtccg gcggatcggc taccgtgacg gcatcggcga cagactcgcc gaaggctcct
     2101 tccgctttgc gagaaagcac gggcaccccg aactctccat gagcgtcaaa cgccaggaac
     2161 tccctgccta cgaccccagg gggctgcagg ggcacggcct tgcctacgca acctcggtcc
     2221 ggggcggcga ccacgtctat gcctacctga tcgcccctga agtgctcggg tcaccggaga
     2281 agctggaccc ctactcaaac gaaggaaagg cagtctggac gaagacattc caggacctca
     2341 ctgccctcat cgactcgtcg gggagctgtc tcttcacctc gttcccgctc ggggcggcgg
     2401 attacggggc catggtctcg gcggtcaccg gctacgagat cgatgccggg gaggcgctcc
     2461 ggatcggcga gcggatctgg aacatgcaga aggtcttcaa cctgaaggcc gggtgcaccc
     2521 gggaggacga taccctgccg ccgcgcctcc tccgggagcc gctcaccgaa ggcggcccga
     2581 aaggccgggt ctgggagcgc cagccgctcc ttgacgagta ctaccgtgcc cggggatggg
     2641 accgggaggg gcgccccaca caggagaggc tccgcgacct cggcatcggg gaggaggcgg
     2701 ttccctctct atgacccgat ctattttttg gtgtgccacc cgcccgtcgc ttcggggatg
     2761 cagtcgatct cgggggagta aggtttgatg tccagcaccg gggtcccgtc cagagcgtcg
     2821 agcccccgga cgacgaggac gttgccctcc cgcccgacca ggtcgacgat ccccaggcca
     2881 atcgggttcg gccgggcagg cgagcgggtc gaaaagaccc ctcgttcccg ggtctccccg
     2941 ggaggtttcg cgaagagcat cccacgctct gcccggtcga gccagtagag cacgatgagg
     3001 tggctgctcc gctccacgtc ctcaagtccg ggagcgtagg cgtcaaggat gtggatctcc
     3061 gcgacggtat ccgcgaggcg cccttgccgg ggggcgtcgc cccggatccg gtagggtgac
     3121 cggacgacgc cgatagggat gcactcgatc gctgtcacac ctacctcctc cgcctctctg
     3181 catcgagtgt ttcgttgcag aggcggtccg cgacctggat gcagggatgc tctcttggaa
     3241 cgctctcgaa cctgacctct gcaaaattac gcacccgctg ccgcacctcg cctgcgaggt
     3301 ctgcaagatg ctctttggtg atgcggtacc ggccggtgag ctggcgaacg accagttctg
     3361 agtccgacct gacttcgagc ctgtcgcagg tgaacgcccg ggcggcctca aggccgttga
     3421 tgacggcgtg gtattcggcc acgttgttgg tcgcggtccc gatgaatccc gatcggctgg
     3481 cgacgactgt tccgtcccgc acgatcacgt atgcccaggc ggcgtctccg gggtttcccc
     3541 gcgatgcgcc atcggtatac agcgtcaccg catcggttgc cgtcaccggt accccctccg
     3601 gtctctctgt ctctgcatca tctaccggct aatcatcgat ctctggaggg ttattggtat
     3661 cgctccccgg gggaggggcc gcccaccgga agacgttaat attccggtgc agtaagggcg
     3721 gatctatgct ggatacgggc cggatcattg aggataaagc gatgcgggac cggctgggtg
     3781 ttttcgaaga ccggaccgat gccgggaggc aactcgcggc gaccctctcg ccgctggaga
     3841 cactcaccga gccggtgatc tgtgctatcc ctgccggagg ggtcccgccg gggcttgagg
     3901 tggcacgggc cttgaaggcg ccgctccgga tggcggtggt gcggaaggtg cagatcccct
     3961 ggaacccgga ggccgggttc ggtgccgtaa cctgggacgg gagggtcttt ctgaaccgtg
     4021 ccctcatgga cgggctcgac ctctcggaga ccgaggtgaa cgctgccatc gcgaaagccc
     4081 aaaagaacgt ggaggagcgg atggagaagt tcggtggagg ccgggaggag ccggggcttg
     4141 aagacagggc cgctatcctg atcgacgacg gcctcgcaac cgggtatacg atgttcgccg
     4201 ccatcgaggc ggtccggacc gggtcacccc gtcaggttgt cgtggcggtt cctaccggct
     4261 ccctccacgc cgtcaacttc atcgcccgga aggcggacct cgtcgtctgc ttaaacatca
     4321 ggaccagccg caccttcgcg gtggcgcagg catacgagcg gtggcacgac atcaccgacc
     4381 cggaggtgca ggaactgctg atccaggccc gggatatggg gctcttctga tacaggagag
     4441 cggtcgggac ccgctctccc ttgccggtcc cttcgccgca actggtttat gcgacctctc
     4501 ccatggggtg ccggaggagt aacaactatg cagaacctga cgatcgaggt cgatttcgac
     4561 cgctttcccc cggagcatac ctgtgatggg gagaacacct ctccgcggat acgggtaatc
     4621 gggtcgaacg cgccctacct tgcggtcatc gtggacgacc cggatgctcc ccggggcacg
     4681 ttcacccact ggcttgcctg gaacattcct gcagccgatg agatccccgg gggcatcccc
     4741 ccggagcgcc gggtctcccg tccggtctct gcggtccagg ggacgaacga cttccaaagg
     4801 accggctacg ccggcccctg cccgccgaag ggtgcgtcgc accgcttctt catccgggtc
     4861 tggggcgccg ggagggaggt cgacctcccg ccgggtgccg accggtcact tcttgaggat
     4921 gcactcgcgg ccgccgccat cgaatacggc gagacgatgg cggtctacga gcgcagaaaa
     4981 acgctcgcgg ctccgccgga taccgggccc cggacctgag accccgcctc caccaccttt
     5041 ttatctcccg gcaccgagaa ccccctcatg ggttccagag tcagtaaact gatcgtctct
     5101 ctccgctccg tagagtttcc cgtctggaac acctgcgacg gccccgatct ctctcccgag
     5161 ctgagggtcg ggggcctcac ggaggagatg cagtcgatgg cggtctttgc gttgaacccc
     5221 tttgaacccg ggtgctcctt cactacctgg atcatctgga acctttcccc ggcacccgtg
     5281 atcccggagg gcattccgcc gcagggtgtc gtgacgactc cggtggacgg tgtccagggg
     5341 acgaacgact atgggaccct cggctggcgt ggcccctgtc ctcccggggg cgagacgcat
     5401 cgctacctct tcaaggtcta cgggcttgac gccatgctcg atctttcggc cggcgcaggc
     5461 aagcacgatc tcatcggtgc aatgcagggg catgtcctcg ggttcggcga gaccgttgcc
     5521 atgtactccc ggtgaccgca caaactcttt ttatatcctg gttccggaag gagagtatcg
     5581 acggagcctg gtatgatgca aaacctggta gtaaaactgg attttgagga gttcccgcag
     5641 gactacacct gcaggggggc gaaccggtcg cctcccatca gggtggaggg gattttagcg
     5701 aacatcaagt cgctcgcggt cctcgcgacg gcgagccccg aggaaggatc gtcgcgggca
     5761 gcgtgggtca tctggaacct ccccgccgtc ccgctgatcc cggaggggct ggcggccgga
     5821 gaggtggtgg agtccccgtt ccatgctgta cagggcacga acgactttgg cagcaccggc
     5881 taccggggcc cctgcccgga ggccgggaag accgaggcat acctcttccg ggtgtacgcg
     5941 ctcgacctcg atcttgcgct cgcgcccggg gcgacctggg aggagatggt ccgggcgatg
     6001 gaggggtcga tgaaccagtc gggagaggtc atagccttcg ccaccggtta aggggtcggg
     6061 agagtccccg cctggagaaa aaccacatga tgcccgtggg gccacaggca taccggatga
     6121 aaatatgtag gtgacccatt ttcatacgaa aggatgccgg cccatctctt tacatgggta
     6181 tgggcgggct ctgtttctcg atgttttttg caataacagc catctcctcg cccagcatgc
     6241 ggatcatgtc gtccatggtg acgataccga tcatctttcc ggcgtcgtcg acgacaggca
     6301 tcctccggat gccgctgtcg gtcatcttcg tgatggcctc gtagatcccc atgttatcct
     6361 ggaacgtgat cacgtcccgg gtcatgacct cctccgccct tacctggcca ggattcttct
     6421 cctgcgccat gacgcggact gcgagatcgc ggtcggtaag tatcccggcg ggccggtaat
     6481 cgccggtgat gacgacgacg cttccgacat tcttctctcc catgatcctt gccacctcca
     6541 ccgccggcgt gtccggtgaa acggcgacga cctgctcacg gcagcacttt acaattccca
     6601 tatacgtttc ctccgttgac gacgactgac tcatgccggt atgatatatg aacgtaaccc
     6661 gtaccgcccg gcccggactc ttttctttat cttgtggggg tgaagggggc ggagcgggaa
     6721 gaccccaccc ttcagggtgg ggatgaaagc gaagccgccc ttcgtcaccc tctcggagag
     6781 attaccggaa actacatctt attataaggc ccaatagtat tcgtatgctc caggcctaca
     6841 agtatcggct gtacccttct cgggg
//
LOCUS       DAJB01000086           10138 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_71476, whole genome shotgun sequence.
ACCESSION   DAJB01000086 DAJB01000000
VERSION     DAJB01000086.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_71476"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    9359..9415
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1898 a         3129 c         3119 g         1933 t
ORIGIN      
        1 cctccagggt tttcgggacc tcgatcggca tctcgccgca gaggatcagg tgatccttcg
       61 cccgcgtcac ggcgacgtag aagaggcgtt tctgctcggc cttctccttc tgccggtact
      121 cccgcttgag gaccttcagg atgggcgcct cctcgcgctc gtggtcgttt gccggattcg
      181 gtatcgtgac cccgagccgc agcccatcct ccaccatgac ggtgttgcca cctgcccgga
      241 acggctcggc aaggtcgggg acgatcacga tcgggaactc gagccccttc gaggcgtgca
      301 ccgtcatgat cgagaccgcg ttcgcagtcg tcaggtcaag cattgcatca ccttcccgct
      361 cgccatcgtc gatgcaggtt ccaagctccc ggacgacctc ggcgagcgtg ccatggccgc
      421 tcgcctccat attccggacg agggcaacca ccttctcgac attcgcggct gcctgttcgc
      481 cgcccggcgt gccaccgagg acgatggaga tccccgaccc ggagacaatc cgggtgagga
      541 ggtcggcggg agagacccgg cgagcgtaca ggagccacga cctgagaagt tgcactgccg
      601 cggtgatgcc cacctcggag tgctcagacg cgaaccgctc cagccgctcc cagagggagt
      661 tctccgggga gccggaccgg gcgacagtgt aaagccgggc gtcggagaaa ccgaagtagg
      721 gcgaccggag cagcccgtag agcgcaacgt cgtccaatgc attctccagg aagcggagga
      781 tgttgtggag gtcgtagacc tcctgccgcc catagaaccc aatacctgcg tggacgtggt
      841 aagggatgcc atagcggacg agcgcccact cgtaggtggc aaggttcgtt cgccgctcaa
      901 ggaggatggc cacatcccga tactccgctg gccggggctc gtcaaggtgc tttccttccc
      961 ggtcccagta gacgcgccgc ttctcgtact cgatgaggtt ctggatcttc cgggcgacca
     1021 tctccgcctc ggcacgccgg ccggactgcc ggccatcggc cttcgggacg aggaggaact
     1081 cgaccgagcc ggcgtcgtcc ttccgaaacg cctccagcgg ttcgtactgg aactcccagg
     1141 gtcgcgcaga ttccgccatc agcgccccga agatagcatt gacaaacccg acgattgcgg
     1201 gcgtgctccg gaagttgacg tcgagcggga tggcctcccc gccgagggtc tgctcgatca
     1261 ggtcgcgggt gcacttaaac tgcgtgacat cggcgtcccg gaagaggtag atggactgct
     1321 tcgggtcgcc gacgacgaag agttttgccg aagtctgcgc gagatcgccg aggatcgagc
     1381 atataatccc gttctgcacg ggatcggtgt cctggaactc gtcaaccaga acaaaccgga
     1441 agcgcctccg gaaatgcgcc tccacgatcg cgtcgtgttc acggaagagc cgatgggttc
     1501 gatcgatcag gtcagcgaag tccagcgcgt tccggtgccg cttctcggta tcaaccgcat
     1561 cggagtaggc cgcaaatacc acgccgaggt cacggaggta gtcgagcgtc gcccgggtga
     1621 acggatcgtc caagtcgatc gtgagcgaaa ggaactcgcc atgcgccttg aggcaggtgt
     1681 tgaggttctt gtaggcttcc cggagcctgt caaggtcgtc cccctgccag ttcggctttc
     1741 tccccatatt cgcccggaac tttgagtcgg cgtggatctc cacgagcgcc ctgaccaccc
     1801 cgcactctcc ggcggcgatg gaggggaggt gcggctcgac tgcgcggagg tagaccaggc
     1861 ccgggtcggc agcgccgggg cagcgggccg cgaggtcctg cagggtactg atcgaggctc
     1921 ctgcagcggc cgcgaagatc gcgagttcct cctcccggca ccgctccaca gccgccctcc
     1981 acgtgtcgag caccacctcc tcactcccgg cgagggcagc gaagaactgc tctgcaacct
     2041 cacggcgcga ggagagccgc tcaagggtgt ttttcagctc gtgcacacca gccatccgga
     2101 gcacaccaac cagcgcgtcc cggcaggttt caggtggctc actgtagacg aatgcatcta
     2161 taacctcgtt tctgagtcgt gctgtttccc gctcatcgag gacggcaaac ccgggggcga
     2221 cgccggcctc gagggggaac tcccggagca cctgggcgca gaaggaatgg aaggttgaga
     2281 tgtttgccca gagaaattcg tcgcggaggc tatcccatcg agcgccctcc ttcttcgcga
     2341 gcgcctcccg gacgcggatc ttcatctctg cggctgcctt ctcggtgaag gtcagcgcaa
     2401 gaatattcgc gacactgaga tcattcctgc tctcgagcag gtcgatatac ttctgcacca
     2461 ggacgtaggt cttcccggtg cccgctccag cggtgacgca cttgctcctc gcgtggtcga
     2521 gggcggcctg cttctgccgg tcagtcagtc ccatcttcac gcacctcctc gatagcgagc
     2581 agccgcaggc tattaaaccg gcagactgtc ttgaagtcac agtaacccgg acaggagtcg
     2641 ggatcggacc ggggcgcgaa ccatccgctc cgtatcccct cgaggcagcg cgagacgtgc
     2701 gcaagggagg tgtcgaccag cgcccgaacg tccgcaacac cgcttcgcga cgatactgag
     2761 aacggtttga actgatcttt cagactcgca ctccagaaaa ccggctggtt ccggatctcc
     2821 ccgcgccgga gggtgtagta acaacccgcg accccctgca tcgccgtcag cgtttcgacc
     2881 gcccggaggt aaagcggcag ctggaacgcc ttgcccgcct cgatattctt gagtgaggga
     2941 tgcgagcttc cggtcttgta gtcagcgatc aggaacctcc cgtcagggag gcagtcgacc
     3001 cggtcgatcc ggccccggat gcggacgacc tccccatccg tcccgccgag cgggatcgcg
     3061 atcgcgtccg gtgtcgatgc cggatcgcag gtccctgccg ccaccgggag gccgaacgag
     3121 agctcgaagg catgcgggac gaaggtcgag gatgcggtct ccacctcgtg ggagaggaag
     3181 cgctcaagca atccccggcc gacgtgcggc gatccgacga ggtgttcctt ctccacgagc
     3241 catgccgggc tgtcgaagag gagacgggcg gtctcatccc ggccgatcgc gaggatctgc
     3301 tggagcgccg cgggataaga cgtctcggtg atcgccccgt ttccgtctcc ccgccaaccg
     3361 gcgtagaacc ggaaagcgat tttgtggagg aggctccccc gttcgagggc ggtcaggtcg
     3421 aggtcgactg ccgggagcgg ctcaagcccg agcacccggg cgaggtagaa tcggaatggg
     3481 cagtctgcat acatttcgag cgctgttggt gagaagactg ccatagcccc gtatcgctct
     3541 gcaagcgtcg cggtgatcgc cggttccccg gtgagcaggc cgtcataggg cgaatcgtat
     3601 gcgccctttc ggcactcgtt ctcgatgatg agccggcgca cagcctcgcc gatcgtgagc
     3661 gccggcggca gtcccgcaac tcctcttgca aactcgccac gggcgagaag ggcaccagcc
     3721 ttccgggctg ccgcgatcct tgatgacggg aaatcgctac caccccaggg ctcggtggag
     3781 atggtttccc tgaccgcatc cacgaacccg gagcgtatca ccggagaccc gccatcagcc
     3841 gaggggtaac tgagataaac ccgtttgtgg gcggcaagga gcgccgcgat gaaatagtag
     3901 cgttcctccc gcaggacgtc cgccttcgag cgcgtcccga gccgccgggt ttcgaggtcg
     3961 gtggtaaacg ggagccgggt agtcaggcgc ggcatcacac cctcgacaag gtcggcaagg
     4021 aagaggtagg ggatagagag atgggtgagc tcccggacgc cgaccacctg gacggcgttc
     4081 cggttccgct tcctcgaaga ccgcgtcccg gcggcgagaa gaccgagcaa ggaggagaac
     4141 tccacgagtg acatctgctg ttcaggcagc acccgggtga accgctcgat agcctccagc
     4201 agatcgacaa atgccctgag gtctcgagct tcccgctcca gcaccccggg atccccctcc
     4261 tccggcatcg ccgggcagtg ccagcgagca aggagcgacc ggtatgtggt gaggtgctca
     4321 gcgagtgtct tttttccttc aaggcatgca agatcggcga agaggcaacg tatcccctct
     4381 cgcacggtct cgatcctggc gatcttctgt gcgagtcgct gttgggcgta atccggggca
     4441 tccagagccg cctgctcctc ttcgagtgtt gcggccagcc cggcgagccg tctgtcccag
     4501 gtgccggcac cggcgacgat ccgggcttcc cgggagagga ggtcgacctc cgaggtcgac
     4561 caggtgaaca ggatatacgg actactcagt agagcgacga catcctcacg ccggtaaccg
     4621 aagacaggaa cagtcagcac atccagcatt gcccgggtaa gcggggatcg gagcagcgcc
     4681 tgcccactgg aagctgcgta cgggagattg aagtcggcga agacctcctc cacgtacagg
     4741 aacgcctgat ccaggtcggg gaacgcgacc gcgatctcgc ctggccggac accgccggcg
     4801 atcaggtcgc aaatctcctg ggcgatcgcc cggacctcgt ccagcccgtc tcgccgttcg
     4861 gcgatgcgga taaatccgcc gctgtccccc ggctcccgcc gggagaagag attggcaaga
     4921 gatgaccggc gctcaccggc gatgacggta tccgggtgca gccacctgcc atcgtccgtg
     4981 aagacggccg gattctccgc gaagggcagg gcatagtgaa actcgtccac ataatcccgc
     5041 agaacgagaa gcaggtctcg ctcacatggc atcggctcaa agaggccata gatgaagact
     5101 gtccggggcc gagcgcccat ctcgataaat tgccgggtcg cccaggcaaa tagagtgctg
     5161 ccgtcgacga ggctgtgatc cttcaggaac tggaggtatg catcgaagag ggcttcgatc
     5221 tcggcgctct tcccactttg tagatcacca agggcgtcgg ggtagtttat cttccgcata
     5281 ataaggacgg agaagagcgt cgcgagttca tcgacggtct tgacaccggc aagcaacggg
     5341 aaccgtgcag cgttggcagt gagaatatcg gtgaggatga ggcgggattc tgcgtccgaa
     5401 atcagcgttt ctatcgttgc attatccaaa aagaccttct gtgcgaatcc ctgcagggtg
     5461 gtgaccgcgc tggcgacgat gggagcaccc tcctcctcga ggcggtgcat gatttcccga
     5521 accagatacg aggttgggac gatgaagacg gtacccaacg gatcttgaac ggcagctttc
     5581 cgaaactgcc gaatgaactc gtctaactga gcaccgggga tatggcggta cagaacggcg
     5641 cggggcattc gataatctct cctggtgaga gattatcggt tcacggacga tgagtgttgt
     5701 tatttgaact ggtgcgggca aaaaaaacga atagtgcaga tctatccact ccgatctcac
     5761 gaagaaagcc aggtaagccg ctcgacaaac acttaaccga tcgacgagtt agcacgatta
     5821 ccgggagagc cagaaggagg gcctgaatcc tccgggcacc atccgcagtt cggctaaacc
     5881 cctccctcga cttttgcaag gcaactcgtt tgaatgtgcc caccctcctc aagtagtggc
     5941 cttaccatgc cgcgaagttc atagaggaca tatccccatc atctcggttg ttgaagtcgt
     6001 atgagagtcg aattgcctgg atcagaagtg gaatatctgc attgatgccc tcaggggacg
     6061 gtaccgagag ggtgaagtct ttcttctgct ccaggatgac ttcggggtag aggagcatac
     6121 atcgccgtcc tcacggaggg cagggcgtgc cacaacctat gtcttcccgc gcctgattgt
     6181 cctcaccgag ggggaccggt tgtgagtgtt ctggtaccag caagcccggt tgcgtatgtc
     6241 aaaaccccga tgcaataccc atttatatac aggatggcgg ttgtgcggca cttctgaccc
     6301 acaagtgaat ttgtgcgaca cgaagtgggg tttcatgccg cacaaaaacg gtagtgcgac
     6361 accggtgacg cacaacgatc ggacctggcc ggccaccggg tcagcggatg gcggcgcagg
     6421 ggtggcggta tgagcgcagg agacgacggg gggttcgcga acgaccgggc attggtgcgg
     6481 gcgtggaagc ggtatccggc gtataaggac tccggggcag ggtggagagg tgctcggcgg
     6541 ggggtgaggg ggatcgacga atgaccatgc catgcggcgt ttccggtgca ttgttgccac
     6601 cgcgatgcgg gatagggcgt tctcccgttc gatcccggcg atccgggctg gctgcgatcg
     6661 gcaggggcgg attccttgct ccctccccct ctttgcatat gtcagggaat agttatcgga
     6721 gagggcggat tgagccccga ttctgctttt taccagggga attgtgcaga acctgaccag
     6781 tagaccctgc tttcaggaag agcattctcc aggagttcaa aggggttgaa ccgggaagaa
     6841 aacatgcttt tgacctgcat cggcctaccc atgcatgtcc gtccgggaaa cgcctgtttg
     6901 ggaatcccgc acgtaatggt tactccagct tcccctccgg cccctgtccg gcgacgtttc
     6961 tgcgatcggc ctcatcgaga atagacctga tcccctgctt gagcaccggg agatcgtcgc
     7021 gtagggtcag gaagattgct tcgtaatcca caccgaagta atggtggacg atcttatccc
     7081 gcattcccgc tgcctccctc caggggatct ccgggtgctc cctggcgagc tccggggaga
     7141 ccctctttgc cgcctcgccg atgatggtca gcatacgctc gatcccggcg cgttccagcc
     7201 ggtgatcccg gagatcttcc gccgatgcga tgccttcgga gaactcctcg atgctgatta
     7261 cggcatcaag gatgtggtga agatacgcga ggtcctccgg cgtcatagat caccacggca
     7321 tcacggtata ttgcatcggc aaggtaaggg ctcacggcgt tctcggtaac gaggtccacc
     7381 ttcatgcgga gagcccgggc gagttcgtcc tcgatacgga caagcgaaaa gaggcttttt
     7441 ttgcgggcga accggaccag gatatcgata tcgctcgccg gaccggcgga tccccgtgca
     7501 taggagccga agatcgcgat ccattcggca tcgttttggg tgagtatcgc gacgatggtc
     7561 tcccgtgctg tcctggttaa tgtgcgtcct gccattgcgc ccgaccgtat ctgtatgctg
     7621 gttggtatga cgctcacccc gtatcagtct tttgaagcgg ctcatgctca ctcacgggtc
     7681 agggcggaca ggattgaaga cggcgtcgga tcggcagggg gacggcgtgc cacgacccat
     7741 gtcttcccgc gcctgctcgc catcaccgag gggggaccgg ttgtgaatac cctgatatct
     7801 gcaaaccagt ttgtgtatat caaaaccccg atccgatata cacaacggac tttgtgtata
     7861 ccagagtata cacaaaccgt accccggggg cggttgtgcg acacccatga cccgcaacaa
     7921 atgttgtgcg acatcacatg cacttttgtg tcgcacaaaa acggtagtgc gacaccggcg
     7981 acggacaacg atcggacccg gccagccgcc gggcctgcgg gcgagggttg aatcgatgag
     8041 tgagttgacc ctcgaactac acgcccgaca gtcgctcacc gcacctggcg gcgctcaaac
     8101 tccgattcta ggaaccgtcg tcactcgaaa accctccggg ttttctcgaa ctcctgccgc
     8161 tcgctacgct cccgccagtc gttcacctca cccccgcccg cccgttccac tcccggacca
     8221 ttcttgcata gcacccctcg cagaccttcg tctgcgcatc ccgcgaacgg tagaccgcct
     8281 tccccgtccc gcagacatcg caccggccga ggttgacctt cgtccgacaa aacgtccggt
     8341 ggtcgaggac tcccgggagc ggttgcacct tcgccggccg tcttgccttc ttcaggcagt
     8401 cgtagcagag gtacttccct ccaccgcgtt tgatcgacga cgtcggcctg ccggttgcag
     8461 acgcgggccc gggttcgtcc ttctccaccg ggagggggat atagtcggcg gggttgaccg
     8521 ggacagtcgt ttgcacattt acactcgttt gcacaccttt gcactctgtt ttgcacctcg
     8581 aaaaaccgct gcctgcagtc tgctccggga tcagcctgat atccactgga atatccagca
     8641 tgtttgcacc gcagtggatt tcacagagcc gagaagccgg gtcgcttgct cctgcagaca
     8701 tactctccgt tcccggtaaa tgatgcaaat tactactctg atctgtacat atacctgtac
     8761 atatctctcc aatgccagaa tcctctctat tatggccgtt atcatcgttt gcacttttcc
     8821 cggaattctg gtgtaaacgt ggtgcaaagg tgcaaacatg cgggccaccg tccctgttgt
     8881 cggggtcgtc gtccagccag accgtggcct gcgccgacca ggcccggtaa acctcctggt
     8941 cgaacgagaa gtaatgctcg cgccgccgga ccgtctcccc ggccccaatc atcccatccg
     9001 tcaccgttgc gtcgatgtaa ctcaccgccg ggcacttctc caggagaccg gcatatgcgg
     9061 cccggttatt tctgtagcca tggaggagcc ggtaggtctg gtggtaggag aggccgaggg
     9121 cgctctggag ctgccggacg gtgaagacct cgactcccat ctgcgcaacc gtcgcgagcg
     9181 ccgccgcctc gttcttcgtc agcttcgtct cctgcccccc ggccgtcccg gagagggcac
     9241 tatacagttt tgcggctgcc gcgaagtctt ccggccgggc gatcaccgcc ccgtccttcc
     9301 cccggtcccg ctggagggag aagagccggg cccggcactt gatcaggtca aagagcatnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgtc
     9421 cttcgccgta ttccggccgg ccgcctcggc ctccttcatg tactcaaaga cccttttgtc
     9481 ctggtcgacg gagtcgtcga tccagaccgt gagcatccgg ttcatcacct ggtcgtcgcc
     9541 gatgtcctcg actttggcga gccaccagac gcaccgggcc gggatggtgc agacccggag
     9601 tttgcgctcg gtcgtgaggg tccggtgctc gatcggctcc cggaagttcg cggtcgcgga
     9661 cttgaggact tcctggaggt cgtcggagag cgagacgtcg tcgaagagga ggacggtgcc
     9721 gggccggagg tcgtcgtcgt agtagagcgc cttgttgctt acggtgccct tgagtttata
     9781 ggccccgggg atgaggttct gcatcgtcgt gcaggcgtgc gttttacctt tgccggagtt
     9841 gccggagacg gcgacgtgga ggccgttcgt gttctcgacc gactgcgagg caagggacat
     9901 caccaggcac tccgcgacgg tccggtcgcc gacgtggctc ttgttgaagg tgtcgaggag
     9961 gaanngggga ggttgtttgc tcctcctgct ccctcttccc cggcgccttc ttctcggccc
    10021 ggacctccgg cttgcgtctc ttccgttcgt acatcccccg gagttccggc cagcgctggg
    10081 ctcccccgcc gcaggtctgg tggtggcagc cgacgaagac ggcgccgttc gcgaactg
//
LOCUS       DAJB01000087           16141 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_73618, whole genome shotgun sequence.
ACCESSION   DAJB01000087 DAJB01000000
VERSION     DAJB01000087.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16141)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16141)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16141
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_73618"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3366 a         4972 c         4933 g         2870 t
ORIGIN      
        1 catatgggaa actcaggggt actcccatcg aaaggagccc ccgattctga tgcaggcgcc
       61 cccaggaacc acgcgggaga actccgatcc caccagacag gacgaaacgg cacgggatat
      121 ctccaccgaa gttgatcggc tcgtgaacga gcggacgcaa gagctccagg cagaagccga
      181 tgcacttcgg cgggagaatg cagagatcaa gcaggccgga gaggcgttga aagaggcata
      241 caccgaactc gacgttcggg tgagggaacg gacggccgaa ctcgggtgct ccaatacccg
      301 acttctggcc gagattggag agcgcgagcg aaccgaagta gttctccggg agagcgaggc
      361 caggtaccaa tccctgttcg attccatcga cgaagggttc tgcatcatcg agatgatctt
      421 cgatgccgcg ggaaagccga tcgattaccg gttcctggag acgaaccggg cgttcgagaa
      481 gcagaccggg atgcatgaag cggcaggaaa gaggatgcgg gagtttgttc cgaaccatga
      541 agagcactgg tttgaaatct atggtgagat tgccacaaca ggcaaaccca aacgctttac
      601 ccgggcagca aaaccgctta tgggagggtg gtacgatgtc tatgccttcc cggttggcgc
      661 gcggaacggc aacagggtgg ccatcctttt caaggacatc accgaagcca aactgaccga
      721 ggacagactg cgcagaggtc gcgaccggct tgccctggca ctggacgcca tcgaggcggg
      781 ggtatcgttc tgggacctca agaccgacgg aatgcagtgg aatccccggc agtatgaact
      841 tctgggatac gcgcccggca ccgtcgagcc cggtctcgcc gcgttggttg accgggtgca
      901 tcccggggat cgccagcacg tcctcgcagc gatcgaccgg agtatccgtg agagagaaga
      961 ttgcatactg gactatcgca tctgcctccc ggacggcgaa gagcgctgga tgcattctgt
     1021 ccgcaggttc gcgtacgatg ctgacggccg tgccgtctca agccacggtc tcatgtacga
     1081 tatcaccgac cggaaacacc acgaagcgat ccggcagcag gcatacgacc agatcgaaca
     1141 taatatggag cagttcgcca ttctgggcga ccatgtccgc cacccgctcc aggttatcat
     1201 ggcaagggcg gacctgatgg aggacgaaga gaccgctgcg agtatccggg agcaggtatg
     1261 ccggatcaac gcgatcgtca ggcagctcga ccggggatgg gtggagtccc atgcgatccg
     1321 tgagtttctc aggcgcaacg acctggcgta aaggtgtaag tgcggctgaa ctgcccatgg
     1381 agagactctg aaaaaacgta ttggatattt agggagaaaa tttgaatccg attacgaaac
     1441 gccgccaaca ggactcgaac ctgtgacatg ctggttaaca gccagccact ctaccaactg
     1501 agttatggcg gcactggaac aagtgctcta taatgtctcg cctgaatcgt attaaacatt
     1561 acgggggttt tccttcttga tggtctcaag aaggccgatc agctcgtcga cctcgccctc
     1621 caggccgtcg aggaggtcgg ccgcgagcgc tgtcgccacc gcaccggccg gggacgcgcg
     1681 gatatacccc tcctgctcga ggatccgcag cgaatagcgg acccggtggt aggggagatc
     1741 caggatctcg gagagcttca tgatgccgat gggctggtgc tccacgaccg cccggatcac
     1801 ctcgaggtgc cttgagacga gttcgatctc ggatttgagt tttgcgagca ttcatccgcc
     1861 tgcagagact atgggttcac gcgcgttacg gctgttcgtt cagccagacc ttcgcccggg
     1921 tgtactgctg ccggaacgat acggagagta tcgccccgac ggcaaggagg agaacgccga
     1981 tcccgaggcc gagggttcct tcgggtgcga accggaggag gacaaagaca cccatggcga
     2041 aactgaggac gatgacgttt aacatgaccg tcagcacggc gaacttagcc ttgaagaccc
     2101 gtttggttgc ctcatccatc attcatactt gaacgcggtg gtaaaagtac ttgtggtttt
     2161 aacgaaagag tgggtagaat gaatggactg gattctgcta tccgggacgc cggagcagca
     2221 gcatacgcgg catacggatc gtctgcggac gccaacgtgc gctacctcac ccggttccgc
     2281 accaccgacc ccgtggtcta cgtggcaaag ccgggcgagc gcgggatgat cgtcgtcccg
     2341 cagatggagc acgagcgggc ggtccgtgaa tcgacggcag cggtcgccac ccgcgccgat
     2401 gccggctacc tcgagtacat caaggccggg gagccgcgct ggcgggcgac cgcccacatg
     2461 atcgccgacc tcgccggcgg ccccatcctc gtccccgcaa ccttccctct cgccctcgcc
     2521 cgggaactcg agtccttccg tccggtcctc cttgatgccg ccggagcggt cgaggggatg
     2581 cgggcggtca agacgccgga ggagatcgag cggatacgct cggtgcagcg ggtcaccgaa
     2641 gcagcgatgg agcgggggat cgcgctcatc cggacctccg tcccccgggg cggcgtcctt
     2701 caccgtgacg gccggcccct cacctccgag gcggtccgtg cggagatgca cgcctacctg
     2761 ctcgcccacg ggtgccgggg gaccgatacc atcgtctcct gcggacccga caccgccctc
     2821 ccgcacagcc ccggtatcgg gccgctccgg gaggacgaac ctatcgtcat cgacatctac
     2881 ccgcaggacg acctctccgg ctaccacgcc gacatgaccc ggacggtcgt gaagggcgag
     2941 ccgacccccg cgatccggga gatgtacgac gccgtccggg acgcaaaggc tctcggcaca
     3001 tcgatggtcc gtgccggggc cgccggcgcc gacctctacc atgcggtggt ggagttcttc
     3061 cgcgaccgcg gctacgagag cgatgcgcgg gggttcaccc acagcctcgg ccacggcgtc
     3121 gggctcgagg tgcacgagga gccgtcgctc ggaccccagg gaggggtgct cgtcgcagga
     3181 aacgtcgtca ccgtcgagcc agggctctac taccccggga ccggcggtgt ccgcctggag
     3241 gatatgggtg cggtgacacc gaccggcttt gaccggttca cccaatacga agaggagctc
     3301 acgttatgat catggacgat gcagtctacg acgcctaccg ggaagcgggg gcactggcac
     3361 gaaaggtgct ccaccagggc gcggggctcg tgaaggaggg ggcaagcctc ctcgagatgg
     3421 ttgaagcaac ggaagcgatg gtgacggagg ggggagcgct ccttgccttc cccttgaacg
     3481 tctccttcaa cgaggccgcg gcgcacgaca ccgccatgcc cggcgacgag cggacgtttg
     3541 cccgcggcga cctcgtcaag gtcgacctcg gggtccagat cgacggctac atcgccgaca
     3601 ccgccatgac tgtcgacctc ggcgaccacg cggtcctcgt cgaggcatcc cgcgcggccc
     3661 tcgaggcggc gatcgccgcg gttcgcccgg ggattaccac cggggagctc gggacgatca
     3721 tccaggcaac gatcgaggag catgggtaca gaccggtcgc aaacctcacc gggcacggcc
     3781 tcgatcgcta cgacctccat gcgaagccca gcatcccgaa cgtcgccatg gccggcgggg
     3841 ccgtcatcga ggaaggaatg gtctttgcga tcgagccctt cgccaccacg gggtcgggga
     3901 aggtcggcga ggcgacgcgc gtggagatct accggcaggt cgcggcccgc cccacccgtc
     3961 tgccgtcggc aaagcgggtc ctcgaagcgg cacggccgcg gcgcggactt ccgttctccc
     4021 gccgctgggt ccccggcgac aaggtcgaga tcgggcttat gactctcgtc cgggcgggga
     4081 tcctccatcc cttcccgatg ctgcacgacg agcccggatc gttcgtctca caggcggaac
     4141 acaccctgat cgtcacgggc gacgggtgcg aggtcaccac ccggtgagtt acgcttattg
     4201 atgacgcgcg cctatatgtc tgttcataga catgaccagt gacacgggta cgcgtgacgt
     4261 cctcgccatc atggaactcc tgctcaccgc ggaggtattc aaccgaaatg aggatctcgg
     4321 catcaacgac ctccgtccgc gctgtcggga gttcttcagc gccggaagca gcgggaacct
     4381 cgaggtgaaa cgtcccctga tcgtgagcga gggggcgatc aagaaggtgc tcggcagtcc
     4441 ggatgccatc tgccaggctg ttcgcgcaaa cccgttcgtc ggttacgacg agttcgggca
     4501 gcgcctgagc ctgccttcgc tcgacgcagc ggcaggatgg ttcctcaaga agggcgggga
     4561 ggagaagatc cgggagaacc ccgtccttgc atacttcttc gaggggaaag acggcaccac
     4621 cgttcagtac caggagatcc gggcaaagaa tccccggttt gaggacacga aggagtacat
     4681 ggaggcaaag gtatcccggc tcatcgccgg gagcgaggag atgcgggaag cccgtgacct
     4741 gatcatcatc agcgcccccg acgaggtcga gttctcgatc tcaaaactgg tctgcactcc
     4801 gcggcaggag gagaccatca agaagatcgg cgtcgccctc gagcaccggg acttcttaaa
     4861 ggagcacggg atctacgagt tcggccggct tctcttcgtc ggcccgcccg gcaccgggaa
     4921 gacatcgctt gcgctcgcga tgtcccgggc gctccacatg ccggtgctcg aggtccgtct
     4981 cgcgatggtc acctcccagt acctgggcga gacgtcgaag aacatcgacc gtatcttcga
     5041 cctcgcaaag aaactcgccc cctgcatcct cttcatcgac gagttcgact ttgtcgcgaa
     5101 gacccgggtc agcgacgacc acggcgcgat gaagcgcgcg gtgaacatgc tcttaaagaa
     5161 catcgaccag atcagcttcg tgaagaacgg cgtcctcctc atcggggcaa cgaaccaccc
     5221 ccgtatcctc gatgaggcgg cctggcggcg ctttgatgag gtggtcgagt ttccgctccc
     5281 cgatcaggcg atgcggcagg cgatcctcaa gaaggtcgcc gccaccatcg agtgcgactg
     5341 cgacttcgcg agcctcgccg cccggaccga ggggttctcg ggctccgacc tccggatgat
     5401 gatgaaggag gcggtgatgt cggcgctgat ggaggaccgg caccgggtcg acccggcgga
     5461 catcgagcag ggcctcatca gagtcgagga gcgcaacatc atccgcggca ttggatactg
     5521 atatgcagat cacgctgctc gggaccggcg acgccatcgg gacgccgaag attgggtgcg
     5581 actgcgagaa ctgccgggca atgacggcgg cgggaaggtc gcggctccgg acgtccctcc
     5641 tcatcgagac cgaggggaag cacattcttg tcgagtcgtc gccggacctg cggcagcagc
     5701 tcctccgggc ctgctcgcct cacatcgatg cggtcctctg gtcccacggc cactacgacc
     5761 acttcatcgg attcggggag ttctaccggg tgcagaagat gccgccggcc tacgcgccgc
     5821 cgccggtgat ggactactgc tccggttacc tccacttcct gccgtttgag aagcacccgg
     5881 tcccggtata tgagccgttc gacctcttcg gcgtcacctt cacgttcttt acggtgaacc
     5941 accccccggt ctacacctgc ggcctcttaa tcgagcacga cgagatcacg gtcgcctaca
     6001 ccgccgatac gcgggaagat atcccggagg caagccggga actcctccgc aacgccgata
     6061 tcgaccttct cttcgtggac gccatcgctc ctgcgggcta caacatccac aagcacatga
     6121 actacgccga ggcgatccgg ttcgcccgcg acgtcggggc gaaggactac cggtgcaccc
     6181 acatgagcca catggtgccg cccgatatgc cgcatgcagg gtacgacatg gagacgttct
     6241 gctgggagta ggcccgcgag tcccacacat acctatatac ccctcccgga accaaacata
     6301 aggtgcgtat gcccatgaaa ctcaaaatcc aggagctgac tgacgacaag gcccggatcc
     6361 tctttgaagg cgaagggaag acctacataa acgcgctcgc cgccgaactc atcaacgatc
     6421 ccggggtgga cgtggcgcag cacagacagg tattcctgtt cgcagacccc gaactcttcg
     6481 tcaccacggt cggcggcagg ccccctcttc tcgcaataac ggatgcggca aagcggcttg
     6541 ccggctacgc cggggagctc ctcgggcagc tcgaagccct cgagaccgca tagacaaaca
     6601 tgaagaagag cgccgaaggc ttcacccgga ttgcccggga agtctttgct cccatctacc
     6661 ccgtcatcgc agagcaggtg ctcgcgtggt cagggatacg ggacggtctc gcactcgacc
     6721 tcgggagcgg ccccggcctc ctctccgtcg cgctggcaca gaagagcaac ctctccgtca
     6781 tcgcactcga tgccgacccg gcgatgtccc ggatcgccca aaagaccgcc gcaggctgcc
     6841 cggaccgagt aaccccggtc accgccgacg tccactgcat gccgatccgc gacaacgccg
     6901 tctcgctcat cgtcagccgc ggctcgatct atttctggaa cgaccgggcg caggcgtttc
     6961 gcgagatcga acgggtgctc cggcccggcg gcgtcgcatt cgccggcggc agcttcggga
     7021 cccccgcact ccgggagacg atctttgcag agatgcggcg gcgcaacccg gactgggacc
     7081 gggatatagt ccggagaagc gggcaggcga cgattgacgt gcttcgccgg gaacttgcgg
     7141 cgagcggtgt tgcccacgct cgcatcagga aagaagaaga ggggttatgg gtggagatcc
     7201 gaaaggacct gagccccgtt attcggcacg ctcggtgaag atgatcgtcc cggcgatccg
     7261 ggtgatcttg atcttcaccg tctgacccgg ttttgcattc gcgacataca tcgtgtagcg
     7321 gtcgatcttt accacgccgt cgccgcgctt cgagaggaac tgcggggtga cgtccatgat
     7381 agcgccctcg gtcggtttcg ccgctgccgt gggatcaaac gtcgatttcc gcttcctgac
     7441 cggccggtgg ccgccgcagg cgtcgcatcg cagcgtcagg acacgttccg tcttaacgag
     7501 gcgggtatcg ggctttccgc actccgaaca gatgacgtag tcatcgacgt agcctttgat
     7561 gatcgcgttg atctgctcct gttcgaactt cccggagaag acggcccgcg tcccctcgat
     7621 cttgccggcg gtgccgagct cgccgaggag atgcttcatc aggtggtcct ggtcgcgccg
     7681 gaggatgcca gcaatctcgg cgaagttctc aaggaccgtg gtcttcccct cgacgaaggc
     7741 acgggcggca ggcaccgtga accgatcttc aaactccgtc ggttcagtga tattggtata
     7801 cgcctccttc agaaggtctt catatgacgg ggtcataccc cactattggt cgcgcgacgt
     7861 caaaaagtga gtacctcgca tcgggacctg cacacgctta tgtaggcagg caactcacca
     7921 ggttttcagt atggtatcgg ctcaggaccc tggattgctg cgaaaagccc tcggacgcga
     7981 cttaaccgac gtcgagctcg cctgctttga aaacctctgg agtgaacact gcagttaccg
     8041 gtctaccaga ccgcttctcc ggacgctgcc gacagccggg agcgaggtgc tgctcgggcc
     8101 gggggatgat gccgcgatcg tgcggttcag cgatacctgt gccctcgcca tcgggatgga
     8161 gagccacaac cacccgagtt acgtggaccc ttacgacggc gccgccaccg gcgtcggcgg
     8221 gatcgtccgc gacctggtcg gagacggccg cgtcaccgct gccaccgacc tctcacgggg
     8281 cgggctcctt gcggcgctcg taaaggtcgc acccggatcg gacgtcacac tcgccggcga
     8341 ccccctggaa gaactcttcg ccgagaccta cggccggttc ctggtcgcgg tcggcgacga
     8401 gtccgccctc gccggagtgg agcaccggat cgtcggtaag gtcggcggcg accgcctcac
     8461 cctccggctg aaggacggga cggtcgtcat cgcccccggc gaactcgagg cggcgctcac
     8521 cgccacgacc cgacggatgc gctactgacc tgtttttacc agcggccgga cgaggttgac
     8581 cagggcctcc accgcctccg gtttttgcac gtcccgcgtc gtgaacgcga ccgcaccccg
     8641 gacgacggca ccccggtccg caagcatcgc cttcaccgtc tcgagggtct ttcccggcgc
     8701 tccgcccgat gtgcagaaga cgaccgcagc ctttccctcg atccccacca gggcggcgac
     8761 ggccgcgttg accgcgggtg tcgggttgcc cgcccatacg ggagttccga cgacgacggc
     8821 gtcgtagccg gagacgtcga tgacggcagg ttcgatatca gctttcttcc cctgcatggc
     8881 acggggagcg cctttgaggt acatactcag cttcgagtag cctgcaaggt cttttacttc
     8941 gatgagatcg gtgccgatca gggcggcgac ctgttcggcg acggcgcggg tagtcccggt
     9001 ctccgagtga tagatgatac agaccgacat accggaaggt gccgccgacg agtataaacc
     9061 ggtatcggcc gggaaaaaga gagtgagttt gtccctaaac atctttgcta aggcgtgcag
     9121 tgccccagtc cagctgatca ggcgagtcgg gaaaagcccg atttcgtcta ctcaggcttt
     9181 cagcaagcag atggaattcg attcgtccac accctccggc acggattccc accaggtatc
     9241 gccacgcact gcccccgccc gggggggacg gggtggggtt acaggaatat gactatctgt
     9301 agatgttgag cctgaacgct gaagatacga ggtcgagaca ggggaggggg acgagccccc
     9361 tccccgtcag ccccaccccc aagggcaata tccaccacgg tccagtctat tgatcgtgtt
     9421 gtggctcaac atgctctcat agatgtttga ggatgtctca gtgttacttc ctgaactgcg
     9481 gcatgaaccc gcggagttcc ttgcccacct gctcaatcat atgctcctcg tccgccctcg
     9541 tcagcgcggt gaagaccggg cggttcacca tattctcaag catccactcc tttgcgaatt
     9601 cgccgctctg gatctcctcg aggacctcct gcattgccgc gtaggtctcc ggcccgatga
     9661 tgcgcggccc tcgggtcagg tcgccgtact gggcggtgtt gctgatcgag tcgcgcatat
     9721 tcgtaaaccc gccctcgtag atcaggtcga cgatgagctt cgtctcgtgc aggacctcga
     9781 ggtaggccat ctcgggcgcg tacccggcgt ccaccagggt ctcgaaccct gccttgatga
     9841 gcgaggtgat cccaccgcag aggaccgcct gctcaccgaa gaggtcggtc tcggtctcct
     9901 cggcaaacgt cgtctcgagc acgaccgcac gggtcgcgcc gatcccccgg gcgtaggcga
     9961 gcgcgatggc gtgcgccttc cccgtatagt cctggtggat ggcgatgagg gccgggacac
    10021 ccttcccctc ctcgtaggtc cgccggacca tgtggccggg ccccttcggg gcgaccatga
    10081 ccacgtcgac ggtgggcggg gggacaatct gcccgtagtg aatgttgaac ccgtgcgaaa
    10141 acatcagaca ggcgttctct ctgatgtagg ggctgatctc ggtgcggtag atggcaccct
    10201 ggttctcgtc gggaaggaga atctggatga tgtcggcacg cttgaccgct tccgccacgg
    10261 agtagacctc taaaccgtct tcagatgcct tctgccagct cctgccgggc cgcaggccga
    10321 tgatgacctg acaaccggaa tcacgcaggt tcagtgcctg tccgcggccc tgggagccat
    10381 agccgatgac ggcgatggtc ttgccctcca gggtgcgggg gtctgcgtca gactcgtagt
    10441 acttctgcac cataggttct cacattcggt tggacagcaa agcagataaa tagtatatac
    10501 gagaggtcta actgccggaa gggtcatttt aaggttgatg aaatgaaaac ctactcccat
    10561 actctattga ggatttgtga tggaactgcc agatgtccag tccagcctgc cggaggttcg
    10621 tatcaacctg acccgggtgg gtgtaaaaaa cgtccaaaaa ctcgttgaag tcgcccgacc
    10681 cggtaagcgg ccagtcatct tcatctctaa cttcgacgtt ttcgtcgacc tccccgggag
    10741 cctcaaaggt gcaaacctct cccggaactt cgaggtgatc gatgatgtac tgcagcaggc
    10801 catcgaagga gatgtaaagg agatcgagga actctgcagc gcggtggcga ggaaactcct
    10861 tgaccggcac gagtacgcgg agcggaccga ggtccggatg cggagcaagt tcatgatccg
    10921 gcgggagacg ccggtcagcg agaccggctg tcacgaggtc gtgaacgttc atgcgagcgc
    10981 aatagctcag aggaacgatg ggaacccgat catccgaaag agcatcggcg ccgaagtgac
    11041 cgggatgacc gcctgcccct gcgcccagaa tatcatgaag gaccatgccc tgcatgtcct
    11101 cgagaacctc ggtgtggcgg gagataaaat cgacgcgttc ttcgaggagg tgccgatggc
    11161 cacgcacaac cagcgcggac ggggtttcct ctgcatcgag atcgacgacg accagcatat
    11221 cagtctcgag aagatcatca agatcctgaa ggattccatg agtgcgcgga tctacgagct
    11281 cctcaagcgg ggcgacgaga gttacgtggt gatggaagcc cacaagaacc cccggttcgt
    11341 cgaagactgt gtccgtgaaa tggcccgaaa ggtgatcacg caattccgga atctccccgg
    11401 agattccgtg gtcaccataa aacagacgaa cgaggagagt atccaccagc acaacgccta
    11461 cgccgaacgg aaagcgacga tagcggagct cgtcgcggag atggacgaag acacactgta
    11521 gaccggatat tattttacat tttttatata tagttaacat atttcaacat tctgtgttac
    11581 attcgatacc tatcgtaaca ttctgggatt tcctgaagcg aaagttataa actctgtttt
    11641 cgtcataatc cttgttgacg tactcacgta cgttttcagt acagaaatgt gtggtaacac
    11701 cagtatagtg ttcagaacac cataatatcg gaatgaggcg atatatttgt cgaaagttgt
    11761 acagatttcc ccaacaacga gacatgaagg ccattcaaag ctcgttctga aggtcaacga
    11821 tgaaggcatc gtcgagcgtg gagactggct cagcatcacc ccggtcaggg gtgtcgagaa
    11881 actcgccata ggcaagacga tggagcaggt tccgaagatc gcatcacgcg tctgcggcat
    11941 ctgtcctatc gcgcacaccc tggcgggtgt cgaggcgatg gaagcatcca tcgggtgcga
    12001 gatccccgag gacgcaaaac tcctgcgcta catcctgcag tgtgccaaca ggatgcacag
    12061 ccacgccctt cataacatcc tgtccctccc ggacatgtac cttcccggta ccgacgtaaa
    12121 gatcaacccg ttcaccaagg aagaaccggt cagaaccgtt gccctccgga tccagcggat
    12181 ccgtgagatt gggcagacca tcggcgagat cgtcggcggc gaggcaatcc acccgagcaa
    12241 cccccgcgtc ggcggcatgt acaagaacat cagcccccgt gcgaaggcga agatctacga
    12301 cctcgcgaaa gaggcccggg tcctcacgca ggcgcagatg gagttcatga tcgcggtctt
    12361 ccggaactac cagaagcgcg actgggctga agtcggcggc gtccaggtcc cgatcacgaa
    12421 ggatctcggc taccacaacc agggctacat ggcaaccgca ccggtctacg gcagctcgag
    12481 cctcgacgag accccgatgt ggttccccga ccggttcacc gaagtccgcc cctgggactg
    12541 gtacatgggt gaggttgaga tcagcgaggc tgacccgaac tacccgatcg gcggcaccac
    12601 ccccgtaggg gacaaagcct ggccccagat ggaagcctgc accggcgtcc cgctctacga
    12661 cggccagccg gtcgaggtcg ggccgcgtgc acgccttgtc cagttcaaga actacgacga
    12721 gaagggtgcc atgggcctgc agatcgcacg ccagatggag ttccccgaga ctgcttacgg
    12781 catcatcgac gcggtggacg cgctcgacac ctccgcatcg gtgctcgcgg acaacatccc
    12841 gcagggcgac ggaaccctcg gatgggccgc gaacgaagcg ccccgtggaa ccgacgtcca
    12901 ccttgccagg gtcaaggacg gccgggtgca gtactacggg atgctcgtcc cgacaacgtg
    12961 gaacttcccc acctgcagcc gtgcactgac cggtgcaccc tggcggctcg cggaagtcat
    13021 catgcgtgca tacgacccct gtgtctcctg tgcgacgcac atgctggtta tcgacgaaga
    13081 caagagactc gtggcccaga aactcattca gtgagcgtac acgcatgcta ttccgtgaga
    13141 tcgtgatcgc agggtgcggc aaccccctct acggagacga cgggttcggc cctgcagtcg
    13201 tcgaagaact ccagaagtta caactgcccg acaacgtcaa ggtattcgat gccggccttg
    13261 gcgcccctca cttcctcttt acgctgatgg aagatgcaga ggtgccggtg aagaagctga
    13321 tcatcatcga tgtcgccgat ttcggcgcta accccggtga tgtaacgaag ctccggcccg
    13381 aagacctgcc gccgggtgcc taccgggacg cccattcgtg ggacctttcc gagccactgc
    13441 aacgactaaa agacgtcatt gacatcacga tcatcgggtg ccagccgaag cgtgtcgcga
    13501 cccaggagtt tgaactaggg ctcactgagg aggttgaggg tgccattccc aaaacagtgc
    13561 gtatcgtact ggaggaaatt ggggtagaat atgggactgc tatcaaccat caaggaacgc
    13621 atctttgggc gtcgcccgga gaaaccgccg gagaatccgg aggcaaaacc ggagaacata
    13681 cctgaggtga aaccagcggc gagccagccg cagacgaagc ctgaggtaaa gcccgaggcg
    13741 aagactgcga gaagtaaacc gcacagaaaa cctgaggaaa agccggaggt gaaaccggag
    13801 gcaaagcctg ctgcaagcca gccgcaggcg aagcccgcgg tgaagccctc tgatgtgaag
    13861 gtaaacgaaa aggtggaagt tgtacaaaag gaggaaaagc ctgtggcaga aaagatttcg
    13921 ataggtgaat tacacctgag cggatgtacg ggatgcctcg tgacgcttgc ggataactac
    13981 gagggtctct tcaagctgct cgatgattat gcggacctgg tctatgcatt gaccctggtc
    14041 gatgtacgcc acgtccccga gatggacgtg tgcctggtcg agggttcgtg ctgtctgaac
    14101 gacaagatct ctgtagatga actgaaagaa gcaagggcga agtcgaaggt gctcgtcgcc
    14161 tacggcgcct gcgcagccta cggcaacatc acgcggtttt gccggggcgg ccagtggaac
    14221 cagcccggtc acgaagcgtt cgtgccgatc agcgaggtcg tcgacgtcga tctctacatc
    14281 ccgtcctgtc ccccgtgccc gcaacaggtc aggaacgtcg ccatcatggc ctacctgctg
    14341 cttcggggca acgacgaaca gaagaacctc gcgaccgcct acctgacgcc gctgatgcag
    14401 cttgcacagc gcggcaacga ggcctgcggc tgcgacctga tgtatgacgt gatcaaccag
    14461 ggactctgca tgggatgcgg gacctgtgcc ggcacctgcc cggtccgcgc catcaccatg
    14521 gagtacggca agccgaacgt gaaccgtgac cagtgcatca agtgcggtgc ctgctacgcg
    14581 cagtgtccgc ggagctggtt caacttcgac gtcatgaaca actatgaggg catcatggat
    14641 gccatcaagg gagccatgca gtgaggtgag aaagatggac gtactcggta attacaagtc
    14701 cgctatctcg gcacgctcga ccgacaagga catcacaaag aagtcccagg acggcggtat
    14761 catcacgacg ctcttcgcct atgcgcttga agagggcatc atcgatggtg ccatcgtcgc
    14821 aggtccgggc gacgagccct ggaagccgga gcccatggtc gcgaccacga aggccgaact
    14881 cctggctgct gccggaacgc gctacaacat cagcccgaac ctctacctga tcaaggaagc
    14941 gacccgcagc tacggtctcg acagggtcgg tatcgtcggt acgccctgcc agatccaggc
    15001 tgtccggaag gttcagctct acccgatcgg catgcgcgac gtcgacgaca agatcgccct
    15061 cgccctcggt atcttctgta tggagaacct ctcctaccag gcgctcgaag ccgtcgttga
    15121 ggaccactgc aaccagaaga tggagtccgt caagaagatg gacatcggca agggcaagtt
    15181 cacggtctac accgaacgcg gtgcagtcag ccagatgccg ctcaagctgc ttgcaaagta
    15241 cgtgcagccc ggctgccacg tctgcctgga ctatgtcgcg aacctcgccg acatctccag
    15301 cgggtccgtg ggcagccccg acggctggag cacggtcttc gtgcggagca tgaagggcaa
    15361 cgcggtctgg gacggcgcta tggcggccgg tctcttcgac acaaagccga tggaccaggt
    15421 caaacccggc ctcgacctcg tcaagaagct tgccactgag aagatcacga agaaccagaa
    15481 gaacgtggac gcacgtaaaa cgatcggtct caaggcggac ggaaccccca agggtctccg
    15541 gaacccctac gagactccct gaaactctct ttttttcgat cgatcggcct cccctcaatc
    15601 gaatatctcc gttgactgag tttttaccgg gtttacagga gatcagttgt ccaaccccgg
    15661 tcaagctgcc cgggtcgtca ctgattgggc atatcaccat gcagcgggcc gtggggatat
    15721 cgccacgcac tgcccccgcc ctttgggcgg ggtggggttt tccgacatcg ctatattatg
    15781 gtggagacga gggggggaac atccccctcg cacctaacgg tgctcaagtt cgctacgctc
    15841 gcactccccg ccccgtcagc ccctccccca atggcgatat ccccacagaa tccgtgcaac
    15901 agggttttac agaatagact catcaggatt atccagcagg gtatacagac ccggaggtcc
    15961 gggctatatc catcaggaaa aatcgagggt gggggccgcc ccgccgcgat ttccaactag
    16021 atcggcggga gatggcagcg gctctgccgt attaggtatc gataaagcgc gtgccgggtg
    16081 ccgtgttcgt attcagtccc tgaacagtac ctggtatttt actgattctg ctccaaaaaa
    16141 g
//
LOCUS       DAJB01000088            5339 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_15754, whole genome shotgun sequence.
ACCESSION   DAJB01000088 DAJB01000000
VERSION     DAJB01000088.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_15754"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1076 a         1542 c         1654 g         1059 t
ORIGIN      
        1 ctctgcgggg tgccgacact ggtgatctgg tcgccgtaaa ggacatgctc ggtctggttg
       61 cctgtagtct ggacccggat ctcgaacagg ccctgcctgc cgacgatctc ctgtgccgtc
      121 gtcatatcca cgccggcgag ttctatccgc acgtactgcg ggtactcgct tcccgtcggg
      181 gtgagcaggt tgatcctcgc gtcctgcatc ccgagggcgt tcaccttatc gttgaggatc
      241 cgtttcacct cgtctgcggt gaacggcgat acgcccttct ggtaggtgac gatcgaggcg
      301 tccgatgccg cgaagagcgg ttctaactcg gcacgggaga cggttttgcg gatctcgaga
      361 tggttctcgt cgacctggat gacatccgtc tcaaggctgg tctgcaggtt ctctataagg
      421 tctccgaccg gtttatcggt cgaataggcc acgataacgg actggaactc catctgcagc
      481 caggagccgc cctggaggtc gagaccgaac tggaggttcc cctcaagtcc cttctccggg
      541 ctcggggggg ccagatagat gcctatgagg gagccgacga ccagcaggag gaccagggcc
      601 acccgccagt ctttgacgag ggttatgatg gtttcgcgat tcatttctta ccgctcctga
      661 ggacgtattc cttgagaatc ccggcgttta gcatccaggt gttcatcatg tcgatgaaga
      721 gcccgatgag gaggacgctt gcgatctcgc tgatgatctg gatctgtccc gccgtggcga
      781 cgacccacat ggcggcgatg gccgcaagtg tcgtggtcgt catgatgatc ccggtcctga
      841 aggccccgga gagtttctcg tcgagtttcc ccttgcgctt taagaggcgc gtggtcagca
      901 ggatgtcgct gtcgacagag taaccgatca gcatcagcag ggccgccgtc gtcccgaggg
      961 agagcgggat gccgacgatc tgcatgatcc cggcggtgat ggcgatatcg gagaaggccg
     1021 agaggacgac cgcacccgcc ggcaccagat cccggaacgc gatgaagacc acgacggcca
     1081 tcccgatgaa cgagaataac agggcaagga aggcctggcc ctggagcgtc tttccaaacg
     1141 tctcgccgat ctggtctatc ttcgcatcgg gatacttctc gttgatgagt tcaacaaggc
     1201 tccggtattg ggtttcttcc atggggccga actggatgta ctttccgttg ccgataccct
     1261 cacccacaga taagagcgga taccctgcga aggctgcctc tattgtctct ctgctatcag
     1321 aggtgaatgc ggtgaccgcc gttcctcccg caaagtcgat gcccggtgtt aagggcaggc
     1381 cggtggtgag cgtggtatag cccagcagga tcccggcaag gaggagaaga acgaggggta
     1441 gcactaccat ctgccgcgga gagtatttgt tgatgtcata tctggcaaat tccatgctgt
     1501 attagtgtta cgattgtgaa aggttaaagg gttgctcatg gatataagaa gggaatcagg
     1561 attcgcctgg atcgcgctgn nnnnnaaccg gagcgttcct taacgggata ccgctccggt
     1621 gcatgtgctc ccacgggggg ggaaccggtt cctgtgaggc ggcgttgcag acgcgcgcag
     1681 gaaaaatcga tccgggggag ggggagtgtc atggaaccgg ggttatctgg tttggaaacg
     1741 cccatcagac cctgtttgtc agacactgcg gcaaacgggt caattatatc ccgcctgatc
     1801 gtatttcgtc agacaattgt cgtcctcctg gcataatctc caagtaaatg attgaaaaga
     1861 aaagatatat ataaaatacc gaatcacaag cacatatgag atcaccctat gagctcaaaa
     1921 aagagataga agctcgactc aaaggttatc tctcacggga ccgggatgga atccggcatg
     1981 agctgctcaa cctcttcgta aaagtaaaat ccctcaccat accccagatc tacgagaagc
     2041 tgcagacgca gttctcagtc agttaccact ccctcgcatc catggtaggc atcatcgcgt
     2101 ccaggatagg catcctgcac gtgaggcgga acgcggaggg gacaaactcc atctacgagt
     2161 tgaaagacca gtacgtggac gtggtggcga atatcctggg gacaacctag tactcgaaaa
     2221 ccatacctct ttttttacac ccagaaccaa tatgaagaac taccatgcag agcgacgttg
     2281 gagagtcagg accgtcacga ggcatctatg tcacggacga cgtccgctcg tatgttctcc
     2341 agaggaaacg cgacttcagg gtgagcacag cctgcagcgg accgatcctc ctgccgacat
     2401 cggtcaaacc ggccaaggcg accgacctgc aggtcccggt cggcgattat accgtctaca
     2461 tctcgaaata tcaggcccgc tacatcgact ccatccaccg ggggatgatt cccatattct
     2521 atgaagactt ctagaccatg agttttgaag aactggatca taccgccgac gtcctcatgc
     2581 gggttcgggg cgcaacctta ggcgagctct tcgccgatgc aggccgggcc atgtttcacg
     2641 taatgtacgg cccctgcgag gaccggggtg tcgagcggca gatctcgctc gaggcggaga
     2701 accttgagtc cctcctcatc gactacctct cggaactgct ctttatcacc gaggtcgaga
     2761 acaccgtatt ctgcaccttc gacgtcgata tccagggcac ccacctcatc gccgttctca
     2821 ggggggagcc tttcgaccct gcacggcact ccgcggggtc gctcgtcaag ggcatatctt
     2881 acttcgggct cgaaatcgtt aaagaagaag aggcttatgt ggtggagatc atcttcgata
     2941 tctgagggat ccctatgctt gaaggaatca ataagatcgg agacctcgaa tgggaggtcc
     3001 ccattggata tgtccccaat atgcgggtat caggacgttt cttcctctcc cggacactgg
     3061 cggagacgct cgaagaaggg gccgtccgcc agctggcgaa tgtcgcaacc ctgccgggga
     3121 ttgtgaacca ttcgcttgcc atgcccgaca ttcactgggg atacgggttc cccatcggcg
     3181 gggttgccgc ctttgacatg accgaagggg tcatctctcc cggcggggtc gggttcgaca
     3241 tcaactgcgg cgtccggctc atcacgacgc ccctgaccga ggccgatctc gccggcaaga
     3301 aacgggaact gatcgagagg ctctttaccg ccgtgccgac cggtgtgggt gccaaaagca
     3361 ccctgcgggt ctcgaacaag gaactctccg gggtcatggt cgacggcgca cgatgggcgg
     3421 ttgaacgcgg gctcggcacc gacgccgacc tcgtccggtg cgagggagag ggggcgatgc
     3481 cgggcgcgga ccccggcgcg gtcagtgcca aagcgcgcca gcggggcgtg ccgcagatcg
     3541 ggacgctcgg cgcgggaaac cacttcctgg aggtccaggt ggcgagggag atcgtcgacc
     3601 ctgaagcggc aaaggtcttc gggatcaccg aggggcaggt ctgtttcatg gtnngctccg
     3661 atcggctccc cggaaggccg cgcctactac ggcggcatgg tctgcgctgc aaattatgcc
     3721 tgggcaaacc ggcaggtcat catgcacgag acccggacgg tctttgcaga gctcttcggg
     3781 atcgactacg atgagatgcg gctcatctac gacgtcgcgc acaacgtcgc caagttcgag
     3841 cgccacgatg tcaacggggc ctcaatggag gtctgtgtcc accgcaaggg tgcgacgcgg
     3901 gcgttcgggc ccggcgcttc cggtatcccc cacgagtatg ccgcggtcgg gcagccggtg
     3961 atcatccccg ggagcatggg gacgtcctcc tacctcctcc acggcacgac gaccgcgatg
     4021 cagaagacct tcgggagcac ctgccacggc tccgggaggg tgctcagccg gtcgaaggcg
     4081 aagaaagagg tcagcgggaa agaactccgg gaacacctcg cgggagaagg catcctggtg
     4141 cgcgctcaca gcgactccgc cctcgcagag gaggcgcccg aggtctataa gccgagccgc
     4201 gaagtggtgc gtgtcgtgca cgaggcagga ctctcgaaga tcgtcgccag gctggagccg
     4261 ctcggagtga tcaaggggtg acctgcagga ccgggctgct ctgggacagc ccccttatgt
     4321 tctcgcggct catcgaggac tgcggcgcct gctgcgaagc ggtcaacccc tacatgctcg
     4381 cctccccctt ctggcgcggg aagttcgtct cgctcatcgt gccgacgggg tttgcaaacc
     4441 cggactactc aaacctcctc cccgctcttc ggtctgcaga ggggcgcatc cggcgtttcg
     4501 tcgagaacgg agggcggctg ctggtcttcg gcgcgggagg ctcgcgggag gacgcctaca
     4561 actggctccc gttcccggta acctacacgt tctcgtacgg gccgcgtgcc gtccggttca
     4621 ccggggagag cgtctacacc tccctgtttt ccgggtatga cctcgacgcc gtcgagtgcg
     4681 acggctcgtt cccggcccac ggcggggaga cgcttgctgc ctcggctgcc ggggaggcgc
     4741 tcctgatcgg gaaggcggtc ggggacggca tgatcctgat ctccagcatc catgagtatc
     4801 cgtcgcgtga atttttgaag gagttctcgt gcggcgagaa ggaaacctta ttctaggtaa
     4861 accatgatga aaagtaggga tacgatcatg gagcaatacg tcatctctgc gtcttcgagc
     4921 tcgcgcgatc tcgctcccgt catgctctgc atccacgagc ttcttggcgg tctgcccgtc
     4981 acggcacgat ctcgtgaccg cccgggggtc aggatcgagg acgggaaggt catagacgag
     5041 gcctatacgg gccctgtcct cgaggaggtc ctggcggaga acgccacatt gagggtcaga
     5101 ccctccgcgg gcccttacaa aggcattccg gtggtggtgg ggccggtgag ggacgacgaa
     5161 ggcagggcca tcggtgcgat cggcatcgtc gatgtgaccg gtatctttga tctggcggat
     5221 tttatggagc agaccacggt tatccggaga caggtgggtg gcgaaggccc gtctccgctc
     5281 ccgaccaggt cgcctgcaac gaaaaggtaa tggctgtcaa ctacccccgg ttgaaaccg
//
LOCUS       DAJB01000089           14740 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_72673, whole genome shotgun sequence.
ACCESSION   DAJB01000089 DAJB01000000
VERSION     DAJB01000089.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_72673"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1734..1806
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4915..5018
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2887 a         4607 c         4517 g         2550 t
ORIGIN      
        1 ccaaatgata gtagagagca aagaccgaat gtgcagacct atcaaattta tatctcacgt
       61 tgggtgaatt ataatagaat acccaagagg atatacctat cgcaggaagg aagggcggct
      121 ccgctttcat ccccggcctg aagaccgggg acttcccgct ccgccccctt cacccccgca
      181 agttaaagac gctcccgatc accgccgtgg ggtggtagtg tgccatcacc cggaccggga
      241 aggtacactt cccctcccgg gttatggtac cggtcattcg tcatccggcg agaattggtg
      301 cccggggtaa aataggtgcc gggggatcag agcgcccagg ctggaatgtc gagggggact
      361 ccgatagccc agaggacgat cgtatagagg cagattatcc agagcacgac gccaataata
      421 tcgagcgccc agcccgaccg aagaaggtcc ccggcattga tgtagccgct cccgtaagcg
      481 acggcgttcg gcggcgtggc gaccgggagc atgaacccta tcgatgcaca taccgcggcg
      541 gtcatcatca ggacgatcgg gtttatgccc acgctgacgg ccgtgatcgc tacgatcggc
      601 atcaggacgg cggccatggc ggtgttcgag gcgatctccg aggcaaacac gacccccagc
      661 gccacgatga ggatcaggag gattatcggg agcccgtgga tgagggtgag cgactcgaca
      721 atcgtcgcgg caagcccgct ctcaaggaac ccgttcgaga gggcgatgcc accgccgaag
      781 aggagcagga tgccccaggg gatcttcacc gcccactccc actccatggt aaagatgccc
      841 ttcctgcggt cgaccgggag gacgaagagg aggagcgccc cgaagatcgc gatggtggag
      901 tctttgatcc ccgggaagat catatccagg cccgggatga cgatcccgtc gatctccttt
      961 gtctgctcga atatccagcc aagggcggtc agggtgaaga cgatgagcgt ccaccgttcc
     1021 cctgcggaga tcgggccgag ctttccgagc tcctcccgaa tgatcccgcg ggcgcccggg
     1081 agagccgacc ccatcccgcg gtaggggacc agggtgagcc atatccaggc aatgaggagg
     1141 aatatcgcgg caaacggaac gccgaacttc atccaggtga agaaatcgat ggtgggtgcg
     1201 tcaggaaaga tcgtcgcgag ctgggcgatg aagatcccgt tcgggggggt cccgatgatc
     1261 gtggcgatcc ccccgatggt cgcggcgtag gggatggaga tgatcaggca ccgggcgagg
     1321 gcctgctccc ccctgttgag gccctcaagt gatttatcgg ccccggggat gatcgtcagg
     1381 atgatcgcga ccgcgatcgg gatcatcatc atcgcggtgg cggtgttcga gatccacatc
     1441 gagaggaacg cagtggcgac catgaacccg agcaccaggc gtttcgggct cgtccccacg
     1501 acccggataa tattgagggc gatgcgccgg tgcaggcccc atcgctgcat cgacatagcg
     1561 atgatgaaac ccccgaggaa gagaaagatc acttcgttgc cgtaggactc cgccaccttc
     1621 accggggaga ggaccccgag caccgggaag aggaccagcg gcagcagtgc ggtcgcctcc
     1681 aggggaataa cgccggtgac ccagaacagg accataagga cggtgactgc cgcnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnaccg ggccgcgcgc cggcgatccc tggccgggcc gcagaggtgg gggctgtgcc
     1861 tgcgccgcac caacctatat cagcagaatc agcatacggg atgtcatggc accagattgg
     1921 atccgcaggc gaggtctcat ggtcctcctc ctcgccgtct gtatactcgc cctctctgtg
     1981 gggcaggccg gagcccgcgg cccggcgatc agcgatatcc agccctacga cacgatcttc
     2041 atctacgaag aggggctcga cctctcgcaa ctccgtaacg cgaccacgga caaccccatc
     2101 acggagctca ggaagtacca gaacgacaac cctgaccggg gggtcacgaa gaacatcccg
     2161 gtcaccgacg acacgaactt cgaggtgcag gacttcctcg taggcgagga gtatggcacc
     2221 tactacgctt tcaacccggt ggacgggacg acagccctcg tgatgatccg cgagccgagg
     2281 atcttcctcg acgtggtgct cgcgaacccc aaccacaacg agccactgac cgggctcacg
     2341 gtctccgaaa acacccggat cgcgttccgg atagcctccc ccgacgtcgg ggccttctac
     2401 caggcgggcg gtgtctaccc ggcaacggtc gacatcgtgc tcaccagtcc gggcggcgcg
     2461 gagagcaccg tcatcagcgg gatcaacctt gccgggcaga acgtcagttc gacccggttc
     2521 tacaccgacg accccggcag gcccggcgcg atcccgctct ccggccttgg tgagcagggg
     2581 acctacaccg tccgggccgt atggaggacg ccggcagcct tcgacgctta cgccgccgac
     2641 tccgaacccg tgacgttcac ggtcgccaac cgtgtcgggg tcgataccgg gacgccgacg
     2701 cccaccgcga ccgtgacggc cacgccgacg accgcagccc cgacgacccc gcccacgacc
     2761 gcaccgacgg cgacggagac gccggtcaca acctcgacga cagcgccggc aacccccacg
     2821 ccgaccgcgg caccgctccc ggtcacggtg gtcgtcgggg cgctcggtct cgccattgtc
     2881 ctcatcgtca ggcgccggtg agcgatcagc aggaccggca gccgcagcca ccggtcgcgt
     2941 cccggatacg gcgaagggcg gcggccgccc acttcgccac acccgggtcg ccatcttcga
     3001 gcgccgacat gagtgccggg accgctcctt tgtccccgat catcccgagg gtgtaggccg
     3061 cccacatccg gaggcgggga tcggcgtcac cgagcgcggc gagaagctcc gggagggcgg
     3121 ccgggccgag ggcggcgagg gccttcgccg ctgccatcct ctcaccgctc tcgccgtcgc
     3181 ggaggatacg gatcagtgct tcgatcccgt ccgtcacggc gacttgttct ccttcttctc
     3241 cggttccgaa tcgttggtgg cgaggatctc gtccaggccc acgtagatcg ggaagacctc
     3301 gtggacgccc gcgagcagga agacccggta gacatcgggc tggagcgagc agagcgccat
     3361 ctccccgtcc gtccgccgca ggtcacggaa cttgccgagg agcacccgga gaccgctgct
     3421 gctgatgtag gccgccttct ccaggttcac gagcgcccgt ctcccacccc gggccagtac
     3481 cccggagatt gccgtatcca ggcgttctgc cgattccgca tcgattctgc cgtcaaccga
     3541 tacgattgcc gtatcgtccc gctcgctgac cgtaacatcc atggttcttc tatcggcgcg
     3601 gaggggaaaa agccgctcgt tttgcaccgg ggcctgagcg ggagcttggc atcgggggtt
     3661 tgcccgttaa aaaagaatgg tcagtgggtc tgccggtagt agtcccatgc atcgactacg
     3721 gtagcgtttg caaagatgca gacaccgtac tcgctgccgt cggggttctt ccggatctcg
     3781 taggtgtgac cctgctccgt gcaccagacc gcggccgggt tcgcgaggcc gacgtttccg
     3841 ccgcaccggg cgcaggccca cgggatatcg atgatatcga tcggcgtccc ccactgctgg
     3901 agggtcacgg tgtcgcgggc gatctccccg acgaaggcaa gaagcatccc gtcgacgcgg
     3961 taggattcat taagtccaag cgggaggtag cgctcgccgt catcggcgac gataccgtag
     4021 aacccgccct cgaggtcgat ataggtgacc gtcccggtct tcgcgacgaa ggtggcgttc
     4081 ccggccgtat cgatcgcgac gacctcgacc ggagtccccc actgctggag ggtcacggta
     4141 ttctcctgca cctctccaac aaacgtcacg ttcatgccgt cgatctgcca ggtctcgttc
     4201 aggtcgagcg ggaggtagcg cctgccgtcg tcggtgacga acccgtaaaa tccaccttcg
     4261 aggtcgatgg aggtgatcgt ggcgttcccg gagacgagca ggacaccgtc gatacgctcg
     4321 atagaccgga tctccaccgg ggttccccac atatggaccc ctgcaccgcc gtcctccacg
     4381 tcggcgctga agagcacccg gagaccgtcg gtccggtact cttccggaag attgccgggc
     4441 agatagttcc tgccgtcgtc ggtgacaatc ccgtagaatc cgccctcgat atcgagatag
     4501 gtgaccgtcc cgttggccgc aaccgaccgg cgggtatcgc ccttctctat gctgacgata
     4561 tcgaccgggg tcccccactg ctggattgtc gctgtctcgc tcgcgacatc gacgacaaac
     4621 atgacccgaa ggccgtcctc ctggaactcc gccgggaggc cggcgggcag gtagcgctca
     4681 ccgtcatcgg cagtgattcc gtagaaccca ccctcgagat cgacgtaggt gaccgtcccg
     4741 ttcccggtga tgcgatcggc ctgcgtatcc tcttcgccga aagccatgca cccgcaggcg
     4801 acgatggccc cgaagagaac aaggacgacg accagatact tcgaatatga acccgtcata
     4861 ggcatcaaca acgatacatt ctccggccgg atgtaagtag attgccttaa ctacnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cggcaccccg acgtccggga
     5041 ggctgatgtt taagttcttc atggcctgct ccactttatc accgaggccg agcgctgtcc
     5101 cgtagaccag ccactcaccc cacatcgaga ggtcggcggg cgagtactgc cggatcaacg
     5161 cgaggtccga gaggaaatag gcgaacgcgt cccactcgag tttctccttg tagtggtcgc
     5221 ccttccagaa gccgaagagc gtcgagggga agagggccgc gatcccgacc tggaccgcga
     5281 cgatgaagga gaggatgccg gccgggatca gcaggaaagc cgcgcccggc gcgaagatga
     5341 acgccaggat cgcaagtccg caggggatgg ccccaaggaa gatgagcggg atgatcatcg
     5401 tccggccatc catgatatac ctccgggaga gcgtggtatc cacgtcccgg gtcagggccg
     5461 taagcgactg ctggtactgc aggatccggt gctggtagcc ggggctccgc cgggcggtct
     5521 cggcaaacgc cgccagttcg gcggtgtcca cgacatggtc gtcggcgata ttgccgagga
     5581 aattgagcac ccgctgctcg taggggtcag aggactctcc cgagacgatc cggaccgtga
     5641 cagcgttgcc ctccggcttc tcggttatca cgatctttcc ctgccggtgg agatcgagga
     5701 ccgtcgcgta gaatccgttg ttgtcgaact cgagggcgtc acccttgaag aggaggttca
     5761 cctgccaggg cttgagcgca gtattcggcg tgaaactcag gtattcaggc accgtgaacg
     5821 atttctccct gccgtagcgg aggtagacgc cgagaaggac aaacggcatc gcgaggacga
     5881 gaacggtagc gaggccatag aggatgctcg ccgccccgta gaggatgggg ggccaccggt
     5941 ttgcgtcggc cgtctgctgc tggacattcg ggacgaactc cccaaagccg ccgactttct
     6001 cggtaaacga cggatcgagg atcagttcga cgtttaacgc gtcgtcctgg ggagcgttcc
     6061 ccgtgatgac gacggcgtca gccgtccgct cgacctcgag ggtcggggga tgcgtgtaga
     6121 cctcctcgat atcccccgcg aacgggagag tgatgcgcag gctccggtag ggaacgtgct
     6181 tgtcgacgag tttcaggttc aggtgcgccc accggtcgtc gtactcgatc ggcggccgga
     6241 cccggtagcg gtactccacc gtgtaggtgc ccggggcgaa gtagccgggg ttgtagaggc
     6301 ccacctcgtt gtcgaacgca agactccgga tcgccgcgag gtcggtggag gtcgcggtcc
     6361 cggccgccgt ccgcacttcg ccctcagagt ccttgacgta gccgatggtc ccgggcgggg
     6421 cggtcatccc gagaaactcg atatgcggcc ggtcgatagc gccgaaggtg agcggtgcgt
     6481 cccagtagca aaagagcatc cgatactctc ccgcggaccg aacatcgtag gtatagcgct
     6541 cgaccagggt tccgttctca taaaagacgg cgtcgtagta ctcgacatcc aggttcccac
     6601 caaagaggcc ggggagcgca gttgctccgg cgaccgccag caccccgacg agcagggtga
     6661 tggcaacgag ggcgaggatc tgctgccgtt cagtcacgcg catggtttat cagaatgata
     6721 tcgtcgggac ccgctcgatc tccgcgccga actccaggta ctcgagcttc gtaaagccca
     6781 tgacgccggc gactaaattc gaggggatcg tgtcggtcat ggtgttgtac tgctgggcga
     6841 tgttgttgta agtgtagcga tgccgggcga tctcgtcttc gatccccnnt cagggtctcc
     6901 ttctcgaagg ctgcgtagct cttcaccgcg ccgaggagct gctcgatcat atccagccgc
     6961 ttcttcatcg cgaccttcac ctgcccgacc gtcgcttcgg cggagttctt gagggagaaa
     7021 aaccggttat agatgcctat tacggctgcc gcgatgccga tgacgaccag caccacgacg
     7081 gcgataagga caagagagat gatgtccacc aagattgtca ccaatacagt atgtgccctg
     7141 cggcatattt aagggatgac ccccgatacg ataggaataa tccgggtttt ccgtatcggc
     7201 cacgctttcc cgggcgaccg gctactccgc tatctcctcg ccggagctca ccatcgccac
     7261 gacgttgccg tctgcatccc ggaggagggc gttgtgccag aggatcgctc gctcccggcc
     7321 gtcccgggtg acgaccgggc tctcctcaaa gggagggggc tcgatctggc cggccaccag
     7381 gcggaggaaa ttttgcgcga gccggtcgcg caggcgctcc gggacgaccg ttgcaaacca
     7441 gttctgcccg gcgaggtctt cctcggcata gcccaggatc tcgctcccct tccggttgac
     7501 gaggtcgatg gtgccgtccg ccccgatcac cgcgatcatc acgccggccg cgtccagata
     7561 gccctgcgcc cggtcacgct cggtgaccag gaggtcctcg gcggcggtgc ggtcggtgac
     7621 gtcctcggcg atcccgagca tgtatttcag catgccccgg gagttgaaga tcgggatctt
     7681 cttcatggag agtgtccgcc cgcccgggag gtggaccttc tcctcaaggg cggccgcccg
     7741 ctctaagagc tcccggtcgc catcggcaaa gaaggccgcc atctccggtg gaaaaagatc
     7801 cgcagcctgc tttcccgcca tctcctcctg tgtcatcccg aggaaggcct cggcggcctt
     7861 attcgagaag acaaacgtgt ttccttccac ctcccggacg aagaccatgc tcgggatgtt
     7921 gttgaccacc gagtagagga acgacttcca ctgggtcact ctcttcgtgg cacgcgtccg
     7981 ttcggtgacg tcacgacaga ccacccggta accggtgaag tcgccgatca tgtcgtagac
     8041 cggcgtcccg ctcacctcaa ggatgacggc actcccgtcc cggtgatgca tcgtccactc
     8101 aaagaggacg ccgggctcgg ggtcggcaaa gagcaccgcg aactgcgcac ggaccaattc
     8161 ggccttctcc ggggccatga agtcgcaggg tgtcttcccg agcatctcct cgggcgcgta
     8221 gccgagaata tcctggcttt tggggctgac ataggtgaac gtgcaggtct cgtcgacgtt
     8281 caggatcagg tcgctgatct tctcgacgag gctccggaag agcaactcgc tctcgcgcag
     8341 ggcctcctcc gcccgccgcc cctcggtgat atcctcgagg atgatggtga cacccggcgc
     8401 cccgtcgttg aagacggtgg gcagaaactt gatccggaag aagagctcct cggcgtgccg
     8461 caggaaccgg agttcttccg tgacctcgcg gccgttcagg gcatcggtga tccggctcca
     8521 tatcagagga tgatcgaacg cggcgagcgg cgatgtctcg ataccattcc cgatgatagc
     8581 atcccgttcg ctcccggcaa acgcacagac ggcatcgttt gcctggacga tccggcgctc
     8641 gcggtcgagg accagcacga tatccgacga gaagttgagc atggcggcga tggggacccg
     8701 ttgcgagagg tagaagactt tggccttccc gtaggtctcc atctccacct ggcccgagac
     8761 gaggaggacg tcaagatatc gggagacagt gttcctgttg atcccgacgg cggcggcaag
     8821 atcagagacc gacatgccgc gcgggtgctc tttcaaggcg ccaagcagga gagagagtgc
     8881 gtcggtagtg ggatccatgt gcaaacagca tttcacgcag agagatatag ggttttgcag
     8941 tgatgcatgc tatgatgcca cacccaatgc cacaccacaa tgcaaggcat tgacccaaaa
     9001 atatattaac cacacagatc aactcatgat ccccagagtc gagagacggg gaatctctct
     9061 gacaagccgg accacaaagg cacggtggtc cgggccggca caaaacggct gggatcggtg
     9121 aacagaacat tcaccacgct gattgcacaa cgtcacggtg taccggagaa accccggtgc
     9181 atccaggcac acctctctaa acacttatac cggcacggtg aacgggaacc cgggcgggag
     9241 gcacaaccgc ccgggatctc ccggagacgc cgcatggtta tcacacaccc gacaatacac
     9301 ctcttttttt gctcgcttag gaagaaacgt cccgaaacag aaagcaacat ctatcttcca
     9361 tcagaacgaa aaactgttca atgagcgggc atgttgagat cctcgagaga agagcaggtg
     9421 ccgtcgatat catcgtggtg aagggacggc tcgacgccgg ctcttcagag aaggcggaag
     9481 agcacatcaa taaggtgctc gatgccgggg ggagaagcct cctcgtcaac ctctgcgacc
     9541 tcgactacat cagcagctcc ggcctccggg tgctgcttgt cgcgctgaag cggctgaaaa
     9601 aggacggcgg agcactccga atcgcctgtg cacaaccgca gatcctcgag gtctttacca
     9661 tggctgggtt tcaccggatc ttctccctct tccccgacga agcggcggcg cttgccgggt
     9721 tcacggcggg gccctgagcg gcatcccggg gtggtgtaga ccggtatggc gttctccatc
     9781 attggcgata tgttcctcgt gctcctgcag atgatctgcg tcatcatggt ggccgcctac
     9841 ctgatcaccc ggacggcgtc gttcacccgg gtgctcgacg gcgtcttcac ctggaaaggc
     9901 caggcgaccc tcatcctcct cttcggggcc ctctccatct acggcaccga gagcggcatc
     9961 accatcctcg gcgccaccgc gaacgtccgc gacctcggcc ccatggtggg ggggctcgcc
    10021 tgcggcccgg tggtggggct tggcgccggc ctgatcggcg ccgcgtaccg cttctccctc
    10081 gggggattca ccgccgttcc atgcgcgacg gcaaccatcc tcgcgggcct cgtcggaggg
    10141 gcaatcttcc ttgccgccgg gcggaagttc atcggaatgc acggcgcggt cctcttcgcc
    10201 gtcggcatgg aggtgttcca catggggatc accctcctcc tctgccggcc gttcgacgag
    10261 gcgctcgagg tcgtcgagga tgtggccttc ccgatgatca tcgccaacgc caccggggtc
    10321 ttcctcttcg cgctcatcat cggaaacctg atcaccgagc ggcagacgaa ggcggaacgg
    10381 gattcctacc tctctgaact cgagcggaag aaggccgaac tccggatcgc gcatgatatc
    10441 cagatgagtt tcctccccga acggctgccg gaggtcccgg gcttcgagct cgccgccctc
    10501 tcgctgccgg caaaagaggt cggcggggac ttctacgacg cgatccccct accggggaac
    10561 cggaccgcct ttgtcatcgc cgatgtttcg ggtaaaggcg tccctgcggc gctcttcatg
    10621 gcgctctcgc ggacggtgct gcgggcgaac gcactggtgc cgaggagcgc ccgcgaagcc
    10681 gtcagtgagg ccaacatgct gatcgccgag gacgcaaagt ccgggatgtt cgtcaccctc
    10741 ttctacgcgg tcgtcgaccc ggcaacgaag atgttcacct acgtcaacgc agggcacaac
    10801 ccgcctctcc tcttccggtc gggcggcggc gggcccgcgg aactgaaggg gaccgggatc
    10861 atcctcgggg tcatgccgga ggccgattac cgggaggaga ctctctacct tgagagcggg
    10921 gacctcattc tcttctacac cgacggcatt accgaggcaa tcaaccccgg cgaggagcag
    10981 ttcggagaga gacggctgat cgagaccgtc acggactgcc gccacctgcc tccggcagag
    11041 atcatcggcc ggatccgcga cgcggtcaca gagttctcag gggaggagcc tcagttcgac
    11101 gacctgaccc tcatggtcct gcgggtggca tgatggcgga actcaccgtg cgggcggatc
    11161 tcacggaact cgcgacgatc gccgactttc tcgccgaagt gctcgccggg agcggcgacg
    11221 agatggtctt tgcggtccag ctcgccgtgg acgaggcctg cagcaacacc atcctctacg
    11281 ggtaccccgg cggggaggcg gggacgatca cgatcacctg caccgtggac gacgacgccg
    11341 tccgcgtgac gatcgccgac gacggcgctg ccttcgaccc gctcagcgcc ccgacaccgc
    11401 tcttcgacac cccggcagaa gagcggccga tcgggggcct cggggtccac ttcatcagga
    11461 cagtgatgga cggcgtggcc tacgcccgcg aaggagagaa aaacctcctc tcgatggaga
    11521 agaagcggcc agcgtcatcc tgacgccgcg agaagcgatc cgagcacccg gggaaggtcc
    11581 tccttcgccg tgacgaccgc ggccgactcc tcgagcatca ggttgacccg ttcgtggtag
    11641 ccgacgacgc ggaagtcggt ccggagcgcg acgatccttt taccaagagc gtacgcatac
    11701 cccatctccc agcaggttcc cgaatcggcg tccgcgccgt cgatgactgc gacaacgata
    11761 tcggcgtcgc ggagcgccag gaggtgctgc gcaaagatag cccgatgctc ctcccggcac
    11821 cgggtgtggc tcgtgtcccc cacctcctgg gggaggtaga catcgaagag gtgcgcttca
    11881 aggaggtcct ggaggacgag gttgtaggcc cgttccgcct ccgaaaagag cggggcggca
    11941 aggtagaccc ggtaacgggc gaactccttc acgtcgaccg ccgggatatc cgggaaccgg
    12001 gcgaggaaca ccccgcgacc gggctgtttt atggacgagt aataggtcgc cgtcaccccg
    12061 caggtggggg aggagtccac cccgacgatc gcgagcggcg gttccccccg ttcacggatg
    12121 acggcgcgga cctccgcttc gagccggtcg aggagcacac caaactcgtc ggtcgcaagc
    12181 cgctcaagat aggatcccgg cggccggtcc gccccgaggt agatcgtctc cgggcagggg
    12241 agcgggaccg tctcgatcga gaagcgccgg cagcgctcaa tggcccgctg gaaatagccg
    12301 aggtcctcat cggttgttat tccgcgggcg cgacacgcgg gattctcgat gcagggggcg
    12361 atgagcacgt acattgatag tacctgggcg gcgtaccata acaagacaga tgataccgct
    12421 ctacgcctac cgggacaaca cctgcgaccc ccggaagtgc acggtgaaga aactggcaca
    12481 ccgcgggctc gccaggatcg tcaccgccat cgcaaggatc ccccggtcga ccctcctcct
    12541 cgatccaacc gcggagcagg cggtctcccc cgccgaccgg aacctccgct cgataacggc
    12601 gctcgactgc tcctgggagg tgctcgatac cggggcggtc gtctcctggc gcaaccgtcg
    12661 tgcgcttccc ttcctcgtgg ccgcaaaccc cgtgaacttc ggcaggccgt tccggctgac
    12721 ctcggtggag gcgatggccg cgacgctcta catcctcggg gagaaggagc aggcacaggc
    12781 gatcctcgct ccgttcgggt ggggcctgcg attcattgag gtgaacgccg acccgctgga
    12841 ggactacgca cgggcgaagg acagcgccga ggttgtggcc ctccaggcgc tctatatgta
    12901 gccggaggct tgcaacgggg atccctatgg gattgcaaac ccgggtacaa cgacctgcag
    12961 agatagagca ctcccccatt catggacaat gaggtccggt tacgcttatc ggagcagaaa
    13021 aagagagcgc accagcgtta aaggaagtgc ggggggtgga tgcccacccg gccgcccgaa
    13081 cgtgctgaat gccgcacgcg acgagtgagt gcgagcacct cccaccggga gacgctccac
    13141 tcgcgtctac agttctctca tgtcatcggg gtaatagttc cccaagttat tagatgacgc
    13201 atgcgatatt tatacaattc tattctaata tcaccaccaa tccccatttt cgccgtaatt
    13261 taccaaaacg ctttagatga gcccggcgtt tccggaacga ttcctgcttc gcacccccag
    13321 atggcgaaac aaacgataaa ttggcggtac attcgggaac gaaccgttcc ctgccatcac
    13381 ccggaaggtg tcccctcgaa gaggcgccgt atcgccctgg catcctcgag catggcgtga
    13441 ttgttgtcga agaagatata cgcccgctcc gggcagatgc cggcgatctt atccctgacg
    13501 ctggtgagtt cgccgtccgt gtagtcatgc cggtaccagc cctcccggcc gtgcatcctg
    13561 aggtagacgg tatcgccggg gaatatccgt tcgcggaagt cgggagagtc gaccgagacc
    13621 aggacggcca cctcatgcag tctccggcac gccccgtcgt caccgagcac cgtagggttc
    13681 ctgatctcca cggcacaccg ctgcccgagg tcgatcgcct ctacaaacgc gatgatgcga
    13741 tcgacgtccg tgaacgccgg tggggcctgg aagaggtaga agacgatgtg gtcgtcgagg
    13801 ggaaggaacc gttcatggaa ccgctcccag acaagaatcg ctttctcgtt gaaccggtgc
    13861 cggtgagtga ccgaccggtt cactttgacg ctccactgca gccccgaccc cgcatcggcc
    13921 cacgaacgaa ctgacttctc cgaagggagc ccataaaaac tcgcgtttaa cttgcggggg
    13981 tgaagggggc ggagcgggaa gtccccggtc ttcaggccgg ggatgaaagc ggagccgccc
    14041 ttcttcactt tcaccctgca cggatcgagt atatcctatc cctgattaac tattctgttg
    14101 tctatgctca tttcctacaa gtatcgggcg tatccagaca caaccacaga gacgcggctg
    14161 attactgcac tcgatacctg taggtggctc tacaacaacc ttctcgaaga atgtaatact
    14221 gcgcgggaac acgggttcac tccgacgatg cgaggaatgc aggcgcggat cgtcacgctg
    14281 aaagaggaga atccctttct gaagggcgta tactcgaaag tgctccagat ggtcaactat
    14341 accctttgga gtaccatcgc cgctctctcc cagacaaaga agaggggacg gaagatcggc
    14401 aaactccggt tcaagagcgc attccggtat cggacgctca actacaacca atcagggttc
    14461 aagatcgacc gggaacacag tacgatcacg ttctcgaaga tcggagcact tccgttcatc
    14521 atgcatcgac cgtataccgg gaaggtgaag agcgtcctga tcacccattc aggggatcga
    14581 tggtatgtga tcgttcaggc ggaacaggag gtctccgaac cgaagagaga gggacgatcc
    14641 atcgggatcg atgtcggtct gaactcgttt gcagtcgata gcgacggggc agtgattgag
    14701 aaccctcggt tccatgagca ctcgcgaaag aagatcaaga
//
LOCUS       DAJB01000090            5884 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_6915, whole genome shotgun sequence.
ACCESSION   DAJB01000090 DAJB01000000
VERSION     DAJB01000090.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_6915"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1222 a         1905 c         1672 g         1085 t
ORIGIN      
        1 gactccttgc tacatctctt tgaaggtgat cggattggcg aagttacggc taacaattct
       61 aatctgggaa gaagaagggg cgtatgtttc caggtgtcag gaactcgaag ttgccagttg
      121 cggcgatacg ccggaggaag ctctggataa tatccgtgaa gcgatcgagc tctacctgga
      181 aaatgcaaga gaacttggga tcctgcagga cattgagccg atcctgatat cccgacataa
      241 gttcacctcg gttatcgagg tcgaggcatg acgaagctgc ctcaggtttc ttccgacgac
      301 gttataaaga aactgatggc gattgggttc gattacgccc ctcaccgagg aaagggaagc
      361 cacgtggccc tctaaaaaac cgaggaaggt ggaaacaaac tgctcgtcat cgttccccaa
      421 cgcgacccta tcccccgagg gacactcatc tcgatcctga aacaggcccg ccttacaaaa
      481 gaaaagtttc ttaatttgtg aagagtcctg tagcaccaga gttatcccca aacgtctgtg
      541 aggctatacg gatcattgcc ccgatcagga ggtgggaggt gcgaccggtc agaaccgtaa
      601 aaacacccat gaatcccccg ccggaataat ccggaaaaca gcggggggtt ccacatcctc
      661 acctttttcc gtccggcccc caaatagtaa gagaccacct gtgtactatc acctgatcct
      721 gaccgataac tgcaacctct gctgcaccta ctgccggggg aaggcgttca ccatcgaccc
      781 gccggaactg cccgggatcg tcattgatga ggacctcccg gcggacctcg atatcgatct
      841 aaaagatctc taccggtttc tcgcgaggga ccccgacgcc gtgctgacct tctacggcgg
      901 ggaaccgctg ctcgccacag acctcatccg cgagatcgcc cgcgacgccc cggtatcggg
      961 gttgatactg cacaccaacg gcacgctcct cgaccgcctc gaacctgcga ccgcgaaccg
     1021 catcggtacg atcctggtct cgatcgacgg tcccgagggg ctcaccgatg cccaccgggg
     1081 ggaggggacg tttggacggg tcatcaggaa ccttcatcac ctcctggaag gcgggtttgc
     1141 cggcgaggtg atcgcccgga tgacagtgac cgaggatacc gatatcgtgg aggccgtccg
     1201 ctacctcacg gagaacgacc gtttcccgtt ctctgctgtc cactggcaga tggacgcgaa
     1261 cttctggaat gattaccccc tgcgggacta cgccgcatgg gcagaagaga gttacaaccc
     1321 cggcatccgt tcgctcgtcg cgttctgggt ggagacgatg cggacggagg gccgggtgct
     1381 ccggtggtat cccttcatgg acccgatgga ggacatgctt ctatcacggc cgagcatgct
     1441 ccggtgcggg tgcggctacg cgaactacag catcatgacc gacgggcata tcgctccctg
     1501 cccgatcatg gtcgggatga aagactacta cgccgggcat atcgccacca ccgacccgct
     1561 ctgcctcccg gtgacgaccg tgggaagccc ctgcacggag tgcggtcttc tcgacttctg
     1621 cggcgggcgg tgcctctact caaacatcat caacccctgg cctgaggagg ggcggcaggt
     1681 cgtctgcgga acggtagaaa acctccataa ggcgctttcg gcagcgctcc cggaggtcag
     1741 ggccctcata gacgccggaa caatccggat ggacgacttc ttccacagga agtacaacag
     1801 ctgcgagatt ataccctgag agaaggggcc gggagaaccc ggtctccccg gactcttact
     1861 ctccagcgct ctccaggagt tcctgggcgc cctccaccat ggtgcgggcg gccttcgcct
     1921 ttgccttcac gatggcatgg ttgccgtctt cgagtgccgc ttctgcctcc tgatagagaa
     1981 tctcggctct atccgtccgc gctctgggtt ttttgacatc ctgcccctcg acggccgcga
     2041 gctcgatctc ctcctggacg gcgagaaggt caccgtagag ttcctccaga agttcgcgca
     2101 gctcttcgat ccgggcctcg ccttcctccc gcttctgccg gccacgctcg cgaagagcct
     2161 cccgggcttc ctcctgccct tcgatgatcg cctgtagttc gttctcgacg tccctgaccg
     2221 cccctgcaag cacctcggca aacgcggtcc ggtcctcatg gctcgtagcc gtcatatgcc
     2281 ccgtatgccc cgccggctgc cgcacccccg agggcaccgc cgatgacgtc gccgacatcc
     2341 cgatcgcccc ggatgatgcc gtcgaggaga ccgccgaggc cggccccggc gatggctccc
     2401 gcgatcccct tctggacctc ttttgcgctc ccggtcgacg gtgtagctca cccggacatt
     2461 ctcctggtca atggtgatct ccatctgtcc ctgctccccg gtatgccgga cgtccatcct
     2521 cgaagacgta tccccgatat tctggtgcgt gatctccgtg cccctgggcg caagctgcga
     2581 catcagccgc tccacgaaat cgcggtaacg ggacgacact ccctgaccat agcaaataaa
     2641 cgccatttat ccctccgttc tgctatccga ttgtaaaggt tcttgaacca gggaataaaa
     2701 accttaccgg acgctgcctc acgcaaaatc gacatgaaag ccgcccaccg gggggaaagg
     2761 caacattcat cccgccgtgg gatgacaacg ctacgacaac catgacgacc atcggtctca
     2821 tcctctgcag aatcgggctc caccggtggg gcaggaagcg gggatacgac gaatactcct
     2881 caaacgtcat cgagtgggag cagcggtgcg aacggtgcgg gaagagaaag cggtgggtcg
     2941 aggcgaagcg ggaccgccgg cggtgacgat tcttagtgta ccttaagacg accgcatctt
     3001 ctcaaacacc ggttgcagca cccctgaccc gccagccacc ctccggcacc acgatctcga
     3061 accgtgcact cttcccgggc tctccggtct cccggatggc gatcccggta gtcgagagga
     3121 tctctcgcac caggaagagc ccgagacccg tgttcttacc aaaaccacgt atgaatatct
     3181 tctctttttc gcctgccggt atcccgggac catcgtcttc aacgacaata acgaggctcc
     3241 tctcccggcg ctgacagtga accccgaccg tcggggccgg cccggcatac aggactgcat
     3301 ccccaaagag gttgtagaaa gccttctcca gcatcggatc ggcatagacc tcaaatccgt
     3361 cgacttcggt aacgagggtg agatcccggg agttgaggcc tgccgccgcc cgggccacgc
     3421 actcacgaag tccctgatac tccggggccc ggacccccag gtccttggta atcccgggtg
     3481 aatgcaatct ggcggctgac gaatccgatg gcctcctgct gccggtccag gtacccgagg
     3541 agaaccgaat cggtcacctg atctctcgtg agccccaggt aaccctgaag gacggtcagc
     3601 tggttcagga tgtcgtgccg ggtgatgctg gagagcaggt gcatcttccg gttcgcggct
     3661 atcagggcag cttgtgtccg cttccggtcg gtgacgtccc ggacaatggc ctgaagctgg
     3721 cgcgggcctt tcacgtccag ccggctcgcg ctcatctcca catcataacg cttcccgctc
     3781 ggtgcagcga tccgccattc gaagaactgc ggctgcccgg catacgctgc ctgcatcttc
     3841 tcaacagcgc tttcaaacgc cgggagaccg tccggctgaa cggggctcga gagttcccag
     3901 ggcgacttcc cgatgaggaa ttcccgggtg cattgaatac cgaaccatct gcaacgctcc
     3961 cagttcccat tatacccagg ttactccata acaacctatc gaaatatcac aacattacta
     4021 gaacgtggaa ctccggagtt agagcccaag tagggatata tttgataata cccgacggtt
     4081 ccgacgacca tcctcgcaca gccgtgtcat gcgacgccgg tcccgccgca gagatcctcc
     4141 gccccgatgc gccccctgcc ggacctgtga ccacggtgcc agccaaccgc ggtgaaccca
     4201 ggccccgctc gggtccgtga aaaaaaggtt actttttgag ttccttcccc gccgagaagg
     4261 ccttcgcgag caccttcccg aacccgtagt actcctgccg catcgagtcg taggcgaacg
     4321 gtcggacctt ctcgatatcg ggcttgccgt ccggcccgag gacgctctcg tccactttca
     4381 cgtccaggat ctcgccgatg aactgggtgt gcatcccgac ctcgaccgtc tggaggagcc
     4441 ggcactcgat aaccaccggg aactccccga catacggggc gtccaccagg tcgcctttca
     4501 ccggcgtcag attggtcgcg gcgaacttat ctgtatcccg gccggagacg ataccgaagt
     4561 agtccgcctc cttcacgtaa tccaccgaag gtatgctgat cgtgaactcg cgccgctcga
     4621 tcaggttctc gtaggtctgc cgggtcttct ggaccgagac cgccaccgcc ggggggcgtg
     4681 aagagcagat gccgccccat gcggcggcca tgacatccgg ccggccggcc ggatcgtagg
     4741 tcccgatgat caggtccggg tgcgggtaga ggagagtccg tgggccaata gattgcttcg
     4801 ccatgggact acgtttcgtc ctccgacccg gataaacctt ttcaaacccc gggagatgcg
     4861 gcgcaacggt gaaaaacagg cgatgggagg cgcggagagc aggtcacacg gcgcccgccg
     4921 cccccttctt ctgctcctgc accgaccgga agaggaagta cccgacgatg gcaaatgtcg
     4981 ccaccggcga gagccaggtg gggatatgga acccgaagct gtccgcgagc atgatcgtcc
     5041 cgagcaccag gatggagtac atcgccccgt tcttgaggta acagtaactc ctgatccggt
     5101 cgacaccccg gacggtcagt tccctgacca cgaaggcccc gataccgttc cctatcagga
     5161 tgaggggaac cgagagggtg aaagcaaagg caccgatgac gccgtcaatc gagaaggtcg
     5221 cgtcgaggac ctcgagatag gcgatcttcg agaggtccga catcccgggt ttcgtcagcc
     5281 gggcctcctg cgcctcggcg ttctgcctga acccgtggac gatgaagaag gcagtggagc
     5341 caagcacggc cgcgaacccc atcatgacgt tgatcgaaag agcgaaccag acaagggcgg
     5401 cgagcaggat cgagacgacc gcatagaacc agacggcctg ccgggagaag aatcgctcgc
     5461 tcctgagccc gcagctcttc tcctccacga agagccagtg gaagaagaga aagacaagga
     5521 acgttccccc gaagatcagg aggatcggcg ccgactcctc gatcgcagcc acgaccgcag
     5581 gatcgctcga gaatgtcgcg agcagcgccc cgacaggccc gagcgacggg gtcgtcgccc
     5641 agacgatcat ccacggcagg agcccccgga ccacgaagac cgcgaagacc agaccccata
     5701 taaggaacca cctgcgggcc ttctccccca tcgtcgcgag gacgtcggcg ttgatgatgg
     5761 cgttatcgat gctcgatacg gtctcgaaga ccacgaggcc cgctacagtc aggatgatca
     5821 gcagccagtc catttcagca gtacacgatc tcgattcgat cagatgtgaa ctaccccgcc
     5881 ctga
//
LOCUS       DAJB01000091           33837 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_75883, whole genome shotgun sequence.
ACCESSION   DAJB01000091 DAJB01000000
VERSION     DAJB01000091.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 33837)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 33837)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..33837
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_75883"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    16499..16529
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20616..20631
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21628..21725
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6366 a        10494 c        10176 g         6654 t
ORIGIN      
        1 cgtagcgcgg gtcgccctcg aagggcttgc cgtccggttt gcagacatcg cagatgaacc
       61 gggcaacccc gttctcgcgg ggccgccagg gcaggtgggc gtatgtggag ggatcgggtc
      121 tgagcaccat gtcggactcc tcgatccgga cgaacccttc gatcgacgat ccgtcaagcc
      181 cgataccgtc ggtcagggcc ttttcagcct gcttcgccgg gatggcgacg ttcttgggca
      241 tgccgagaag gtcggtgaac tgcaggcgga ggaatcggac gttgtcctgt tcgatacgct
      301 cgagcatcga tgagatgatc tcgggagaca tatggaagga aacttccttc caatagtatt
      361 tatctctggc ggatcaacat ctatcagagc gttcccccaa ccactccgga cccgccgggg
      421 agggacagag ggggcggatc caccgtgatg gcaatgtgcg gcataattgg cgtaatggac
      481 agatctcgcc ggacgatgaa tggttccggc atccgacagg ccctctccat gatgaacgag
      541 cgcggcagcg gcgagggggc ggggtacgta gcctacggca tctaccccga ctaccgggac
      601 tgctacgccc tccatgtctt cttcgacaac atccgtgaga acaaaccggt cctcgacgcg
      661 gcgctcgagc agtgggggac gatcgagcac gacgaggcga tccccacctg cgttcagccg
      721 aacctccggg cagtccacac cccctggcgc tacttcttca ggccggaccc ctccctcgcg
      781 atgccgggca gcctcacccc cgaagacgat atcgtcagcg ccctggtgat gcgggtcaac
      841 gccgcccgcc gtggcgcact catcttctcc tccggcaaag acctcggtgt cttcaaggcc
      901 ggcggctggc ccgaggacgt ggcggactac taccggatag aggactacga gggctacacc
      961 tggctcgcgc acaaccgcta cccgacgaac acccccggct ggtggggtgg tgcgcacccg
     1021 ttcaacctcc tcgactggag cgtcgtgcac aacggcgaga tcacctcgta tgggaccaac
     1081 cggcgctaca tcgagagttt cggctacacc tgcacgatgt tcaccgacac cgaggtcgtc
     1141 gcctacttgg tcgacctcct ggtgcggcgg cacgggcttg acatcgacct cgccgtccgg
     1201 gcgctcgccc cgcccttctg ggaggagatc gaccggatgc ccgggaccga gcgggaatgg
     1261 aactctgcca tcaggcttgc ctatgccccc gcgatgatga acggaccgtt tgccatcgtg
     1321 gctgcgaacg ccgagatgat ggtcgggttc accgaccgga gcaaactccg gccgatggtc
     1381 gtcggcgagt gcggcgaccg cctctacatc tcaagcgagg aggcggctat ccgggcgatg
     1441 gagcccaacg tcgaatcgat catgatgccg gccgcagggg agccggtgat cgggagggtt
     1501 gccccgtgac gatcggaagt cttcctcccc ggtaccggat cagcatcgac cgggtgcagt
     1561 gcatggagtg cgggcggtgt atcgagaact gttcgtatgg tgtcttccgg caggacaacg
     1621 gccggatcct catcaactcc cggaactgca ctgcctgcca ccgctgcatc gcctgctgcc
     1681 cccgggacgc catcatgctt gaggagcacc cctgcgacta ccggagccat cccctctgga
     1741 cccgcgaagc ccgggaggcg atctacaacc aggcccggac ggggaagatc atcctcgccg
     1801 ggatgggcaa cgcactcgat tacccggtca tcttcgaccg cctggttctg gacgcctgcc
     1861 aggtcacgaa ccccagtctc gacccgctcc gcgagccgat cgaactcgtg acccacctcg
     1921 ggaagaaacc ggcgaagctg gacctccggc ggcgggagaa cggcgacgtc gaactccgga
     1981 cccgtctcac cccgaatctc cgcctcgaga ccccgatcat gatcgggcat atgagttacg
     2041 gggcgatcag cctcaacgcc cagatggcca tggcgcgggc ggcaaaagaa gtcgggacgt
     2101 tcatgggcac cggggaaggc ggcctgcact ccgctatcta cccctaccag gacaacatga
     2161 tcgtccaggt cgcgtccggc cggttcggcg tgaacatcga ttacctggag cgcggggccg
     2221 ccatcgagat caagatcggt cagggtgcaa aaccggggat cggcgggcac ctccccgggg
     2281 agaaggtctg caaggaagtc tcccgcacac ggatgatccc ggaaggaagc gatgcgatca
     2341 gccctgcgcc gcaccatgac atctacagca tcgaagacct tgcccagctc gtccggggcc
     2401 tcaaggaggc gacggagtgg aagaaaccgg tcttcgtcaa gatcgccgcc gtccataaca
     2461 ccgccgccat cgccgccggg atcgcccgtt cgccggctga tgcggtcgtc atcgacgggt
     2521 tccggggtgg gaccggtgca gcgccgaagg tcttccgcga ccacgtcggc atcccgatcg
     2581 aggcggctgt cgcgagcgtg gataaaaaac ttcgcgatca ggggatcaga aacgagatct
     2641 ccatcattgc aagcgggggt atccggggga gcgccgatgt tgcgaaggcg atcgccctcg
     2701 gagcggatgc ggtctacatc gggaccgcag cgctcgtggc gatgggctgc cgggtctgcg
     2761 ggaactgtta ccggggcctc tgcccctggg gcatcgctac ccagcgccag gacctcgtgg
     2821 accgcttaga ccccgatacc gcatcgacgc aggtggccaa cctgctccac gcgtggacgc
     2881 tcgagctctc cgaactgctc ggcgcggccg ggatcaacag catcgagagc ctgcggggca
     2941 accgcgaccg gctccggggc tatatgctcg atgagagcct gatgcaggtg ctcgatgtcg
     3001 agtcggtggg ggcgtaacgc cctcacgacg tcgcgacttc gacgcactct tttttcgtcc
     3061 cgatccgggc gagcgccccg cacatcgccg gaacgtaggc aagcgccttg cccgagtcca
     3121 ccatgatcga tgcgaactcg tccatccggg gcgagacgac catgcaggtg tccggtatca
     3181 cccgggcacc gctccgctcg atggcggcca caaggccggc gttctcctgc gcgaccccat
     3241 gggcggcgaa gatgtagaaa ggcttcgtcg tcgtctttcc cctgaggaga tccgcgaggc
     3301 cgcggagttc gtcgggggag cagtgcgggc acccgaccgc gaccgcctcg acgggggtct
     3361 cagcaaagag ggcttcgacc tcgtcctggg ttactgtcac ccggtcgagg tcggcggtat
     3421 cgaacgaaaa gaccctcgct tccggggtta tcttatcgac gtggaagaga gcgaccgcgc
     3481 cggtcgcggc catcgcagcg ccgagcgcct tcaactggtc gcggcccggc cggataccgg
     3541 aaaagatcgg tatccggttc cccaccttct ttccggcgac gtgcccgatg gcgccccagt
     3601 ggtcggaccg cgggcccgtc ccgctctcga cctcgacgac gacctgcggc cgccggttcg
     3661 cgacgatgtg gaggccgtag tatggggtct tcccgatgat cgccgccgca agggcgctcg
     3721 gtccgccttc gcggttggtc cgggcgccga gaaccgagtt tgcgtaggcg accgccgagg
     3781 actccgacca ggccaggtgc tccccgtact ccgtgatgcg gaggtagtag ggcgtgcagg
     3841 tgcactcgag ccggatcccg agcctccggt aggtctcgac gacctccgtc tggtggtggg
     3901 cgaactcctc agcgatcccg aactcccgcc acccctcctg cggcatcccg atcgggttca
     3961 agacggtcgg gaccgccacc cgggcgttga ggctctgcag ccaggcaagc ccccacctcc
     4021 cgatggtctt atacgatgca ccgctcacct gggcgctcgc aatcgggacg agcctctcgg
     4081 cgtcgtagac tcccccgagc gcgaccagga tctccatcat cttctggcgt gtctcgccga
     4141 actcgccggc gagcaccttc tcgtcatcgt tatcaaggta caactacagc cactccgctc
     4201 tcctgaattc gtcctctctc cccataacca tcgtggcatc gaccccgacc ttcacgttcg
     4261 tcccatcaga gaggcgcgtc gggtcaagcg acgagccccg caccccggtg atgaccatga
     4321 tgtcggtatc gccccgcacc cgtgtcgcga tcgcgtactc gacgtcgtgg gggtcgtgga
     4381 tgttgatgtc ctcgtccacc accacgacgt gctttaacga ggtatgggcg gcaaacgccg
     4441 ccattatggc gtttttggcg tctccttcgg tgttcttccg gatctggacc accgcgtgga
     4501 ggtagccggc gccgcccttc gtgaggagga cgtctttgac cgtcgtcacc tcgccgacgg
     4561 agcggtagat cctgggctca tagggcgctc ccatcaggag tttgtgctcg tcgccggcgg
     4621 ggaggatgcc gtggaagatg gggtcctcct tgcagtacat ccgggtgaac tcgatcaccg
     4681 gctgctgccg gacagggtcg taggtgccgg tgatatcgac gaacggccct tccgccgctt
     4741 tttcggcccc gatatatcca tcgagcacga tctcggcgtc ggggacccgg acgccgttct
     4801 cgcacacccg gacggggagt tcgccgccca tcagctccgc cgcgtaagcg agttcctttc
     4861 cttccgggac gcgggtgcag gcggcaaacg tcacgagcgg gtgggtcccg acggtgacgg
     4921 cgatcgggag tttctccccc cgggcgagcg ccgccttaag gagggtgtgg gtgtgcctcc
     4981 cctccaccag gcgggcgacg acccggtggt catcgaggac catcatccgg tggatggagg
     5041 cgttctcgac acccccgtag cgcgaaaaga ctatcccgga cgtgaagtag cgcccggcgt
     5101 cccccgggaa gtgcttcatg accgggaggc gggagaggtc tgcgggaacg ccgccctgca
     5161 accgcccggc atccaccacg cggccgctgt aggtggcggc cgcaaggtgt ctgaccagat
     5221 cgcgctcctc cacgccgagc gccgctgcaa gcgatcgcct gtcgccgagg aggttcatca
     5281 ctccccggtg gccgtcgagg tcgtggaaga agaggatctt atcggtccgg cttgacatcc
     5341 gcggtgcctc gtagacggtc gagcaggggc tctcgacatc ctcgaccctg ccctggtttc
     5401 gcatcatcgc tatgaaatca cgcatcgtat ccgctccatc gggatccaag acggtgcttg
     5461 acccccatgt ggtcgagcac ccgggcgacc accatatcga cgaggtcgtc gatcgtctcc
     5521 gggtgctggt agaaaggagg acttgcgacc atcacggtcg cgccggcctc atcggccgcg
     5581 agcatattct ttaagtggat ccgcgagagc ggcatctccc ggaggaggag gtggaggggc
     5641 cgcctctcct tgaggcagac gtccgctgcc cgggtgatca ggttgtcggc aaacccgttg
     5701 ctgaccgccg caagcgtctt catgctgcag gggacgatcg ccatcccgtc gtagcagaac
     5761 gacccgctgg cgatatcggc cgcgaggttg ctgttctcgg cgtatatggc gtcgaacccc
     5821 gagaggtcca cgtgctcgat cccggctatc tgccgggcgg tatcggagat gacgatatgc
     5881 accgttgcct gatcgcagag cacctcgagc aggcggtggg cgtatatgac cccgcttgct
     5941 ccggtgaccc cgacgacaaa ttccttcttc atctgctctc catctcttat gtcactatga
     6001 gtttatcctg tttcgtcgcc cgcttcaccc ggaggacctc catcgccgcc tgctccacgg
     6061 tatggcggtg ctgctcggtg gtgtagatcc cgaggcgcca ttgctgccgc cgggtgtcgt
     6121 ttaaggtgtt gatgaggggc gagaccccct cgatatccac catcgcgtgg ctgttcctga
     6181 cccggacctc catcgagagg gcgcggggga gccgggggat gtccacgagg acgcagtccc
     6241 ccgggacccc tgcggtctgt gcgatggcaa gggcgaggtc ccgctcgcgg gcggccccga
     6301 ggtcctgctg gagtgctgcg gcgttcaccc ggtcttcgcc gacgtagagc gccctcttgt
     6361 agaggtcccg gacatagacc cggcgggcga ggtcgcgggt gattgcgtgt tccgagtgct
     6421 tcagccgctc catgcaggcg gcgtcgtcca tctgcacgag ttcgcgggcg tccgcacccg
     6481 ggacgttctg cacctcctcg cggagggcgt gaacgaacat gctcgtcgcg atccggctca
     6541 cgtggtggaa gtagaccgcc gggcgcatca gggtccgggc gatcaggagc gactcggcgg
     6601 cgttgatgcc gccttcgtgc agggcgatac cggattcggc caggatcgtc gaccggatca
     6661 gccggtgggc gtcgacggtc ccgtagggga cgccggtgta gtgggcgtcc cgcagcaggt
     6721 agtccatccg gtcgacgtcg aggctcccgt ggatgatgct cgcgaggcgg tgctctcctg
     6781 cgacgacggc gcagacctcg gcgggctcga gcccctccgc ttcgaggatc ccggcgattg
     6841 tcccctcgca gacgagatga tcgatctggt ggtggcatct ccccgtgaac tcctccatca
     6901 ccggttcggt gacgtgggag aaggggccgt ggccgatgtc gtggaggagg gcggccgtcg
     6961 cgacgagcct cgtctcgccc ttatcaagcc cgagctcctt gcacatgagg cctgcgaggt
     7021 gcatcgtccc gagcgagtgc tcaaagcggg tatggtttgc cccggggtag acgaggtttg
     7081 caaacccgag ctgggtgatg tggcggagcc gctggacaac tcccgagtcc agcagccgga
     7141 gcgcgagcgc atccgcctcg acgtagccgt gcaccggatc tttgatgatc ttcatctgtt
     7201 cggttaagat tcttgggaga tgagtgataa aggtatttgc caacgcgcgg atcgtgagat
     7261 tactgcgctc ccgtgggtct ctggtattct ccggggtttg tgacgctcat gcgccggtcc
     7321 aggatgttcc ggcagcgaag ggccgggtag atccgttttc ggatggatgg atctatgcgg
     7381 gcagttctgg aggagatcaa aggcgtctac gggtcctaat ggtgccggat agctatacta
     7441 gaatccgtgg agatcagcaa ggctgcgctc gcagaaacgt cttgccggtt tatagatctg
     7501 ttcgatgttt ttgaagaacc ctatgtccca cccatgctcg ttttgcgcaa tccgtccttt
     7561 gaaaacgccc cctgcaatct tctccaggcc atcttcaata tatatggcgg caggctgatc
     7621 ttcctttatg ccgtgttgga ttaacgtgct taaacgctgg aactccatcg aaaccgactg
     7681 gatcagatag tgttcatttt ggttttcttt aacaaaaaca aatatgtgaa tggaactttc
     7741 atccatcatc aggtcgctaa ggttgacatc tcttatgggc ccgccttcga tcccgggatc
     7801 ctgtctatcg gaataatctt ctgagattct ggtgtttaag tagccattcg cccgcaggta
     7861 gtctctcagt gcaagcagat ccctcttgcg tgactgctgg aatgaaccat agattgcgat
     7921 gttggtattt tccttgaaat gccggtgtaa tagaactctg tctgcgattt cgtcgggtat
     7981 cagaactcta tggttcattt tagaggtttt tgtctgcgga atagataacg atgggggttg
     8041 ctgtaatgta actgatgggc caggaaagat tgatataagt tacaataata gaactattct
     8101 tgatggaggt gtcagcatgg accggacaac tgtggaggct tacttaaacc gggtgcctgt
     8161 tgaaattcgt gagtccgtga tggaactatc gacggatgcc agatgggctg tatacatagc
     8221 gcttaccctc gggaaggaga tgtatttcaa cgaactcaag gataccttcg gggcgaaccc
     8281 gagtgagatg gaccgtatcc tgaagtctct ggttgatggg ggtctcatcg cgaagaaggt
     8341 gagaagactg gaggatatcg gcgatcggag gaaggtgtat tacacatcga cagactatgg
     8401 aaaaaagatg ttaactgccc tgtgtgacgt ggtgcttcct cctgagagcc tggtcaagca
     8461 gtataagccc acttcgagcg atagcggaaa ctcctgcacc atgcatacgg gaactgtgaa
     8521 aagaagcggt gagaccgatc ccgagaactg gagattcgac cagacccctg ccagaagaga
     8581 cgccttcaat acaaaaccaa caatcaccgg cgccaaacag gcagagacga aggtgtgagt
     8641 tcaacatggc tgcagaagac caggagcaag ggttgaaact tgagatcgtg atggaccctg
     8701 aactcaagac agttcacatc gacggcgcta tcgtaacgtt aaacccaaat ttcggccagc
     8761 tggcactgac atacgatcgg cctgaattca aagttcatcc aaacggcgac atttacaccg
     8821 ataaagttca aaaacgggta ataattgatg caaggttgtc gcctgaggca tttagagccc
     8881 tcgcctgcac catgatggaa catgtgcggc aatatgatga gtggctcaaa cagcagaaat
     8941 aaatcggtgt ccctggtgca ggggggcaac tcctttttcc ctgctatgat ctgattttaa
     9001 ccgccgtgta aaccgaagct ccgcggccat gatcgtaatg gccactgcca gacgccatgt
     9061 ttgcaggttc cttttctttc acgactgtaa ctgctttccc ggctcccggt gtacctgaac
     9121 ctcgtagatg gacctgcttg cctggcactg cgacgccggc caggaatcat gtcttgttag
     9181 cgaggccgta acttatggga tgacctcgta gggagccagg aacccgggtg ggcgcgccca
     9241 tcaacactca acccccgccc ggccccacac ccctccccac cacagcaaat gaaactgtta
     9301 accccccgga caccccacat aaatgacatt cagccatgat caccttcctc tccggcggaa
     9361 ccggagtacc tgcgctcatc aggggcgccc ggcagatcct ctacgacagc gagatcgccg
     9421 tggtcgcgaa taccgccgag gacctctggg tctcggggag ccactgtgcg ccggatatcg
     9481 acaccgccat cttcctcttt gccggcatcc tcgatcccgg ccggtggtgg gggatcaagg
     9541 gggataccta cgctacccac aactacctcc cgaaggtggc gggcggcgaa cccttcgcga
     9601 tcggcgaccg tgcccgggcc gtccagatcg cacgggcgac gctcctcaag aagggatgga
     9661 ccctgacgga ggcggtgcag gcgcagagcc gctcactcaa catcggggcg accgtcctcc
     9721 cggtgacgga cgccgattcc gccctcttcg tcgataccgg caccgaacgc ctgccgctct
     9781 ttacctactg gatggccgcc gacatgggtg cggaggtgcg ggaactcgtc aggcccgagc
     9841 ccccggcagt gacggacgag gtgcgggcgg cgatcgaggc aagcgacgcc gtggtgatcg
     9901 gtgccggaaa ccccgcccgg agcatcctcc ccatcctcga ctgcgccggc atgcgccgcc
     9961 tcctccgcga tacgttcgtc gtcgcggtct cgccgttccg gggcggcagt gcgccggacc
    10021 cgaaagatgc cgccctcatg tatgccatcg gcgaaacccc gacctccccg gcggtcttcc
    10081 ggctctatga ggatatcgtc gacgtcttcg tccaggacct ccacgacccc gacgaggtcc
    10141 ccggatcgct ccgtctcgag acccgtctcc tgcatacccg gcaggccgaa tcgctcgcct
    10201 gggacatcat ggcggtcata cggcacgcgg tttcgtaaac cgggatgatt gtacttcccg
    10261 gcctaattga caaattacat atacggttgc atttgatacg ctaattattc atgataactc
    10321 tcctctcggg cgggaccgga accccgaagc tcctccgcgg catgcaggag ctgctggatg
    10381 agcgggatat tacggtcatc gtcaacacgg ccgaggacac gtggctctcc ggcaaccacc
    10441 tctctcccga cctcgatacg gtcatgtacc tctttgcagg catcctcgac accggccagt
    10501 ggtgggggat ccgcaacgac tcctacatca cccacgacct cctcgcgaga ctcggcgtcg
    10561 aggagtacat cgccgtcggg gaccaggacc gggcgatcca cgtcgcgcga ggagagatgc
    10621 tccggagcgg catacgcctc accgaggcga ctgcggccct ctgcaggagc ctggccgtcc
    10681 gggcgaccgt cctcccgatg accgactccc ccgtgacgac ctacgtccgc accgaccagg
    10741 gagagataca cttccaggag tactgggtga agcaccgcgg agacctggcg atcgacggcg
    10801 tgacccggcg gttcccggag ccgccggtcg cgaccggcga ggttatccgg gcgatcgagg
    10861 cgagcgacgc cgtggtgatc gggccgagca acccggtcac gagcatatcg ccgatcctcg
    10921 agtgcgccgg ggtgagagag gcgctccgcc accagaacgt catcgcggtc agccccttca
    10981 tcggcgatgc tccggtcagc ggcccggcgt cggccctgat gcgggccttc ggcaaagaac
    11041 cctcgtcggc aggaacctgc ggtctctacg aggactttgt ggacgtattc atccaggacg
    11101 tccgggaccc ggtcgggatc aagggggctc tccgcctcga caccctcatg atcaaccggg
    11161 gcaagagcct ggacctcgcc aagaacatac tgacactgat ataccggtgt taacaccctt
    11221 cactctttct tctgcttctc ccggtaatcc tcgattgccg cgtggagcgc gtccgccgcg
    11281 aggttcgagc agtgcatctt tttgggcggc agcccctcga gttcggtcgc cacgtcgtcg
    11341 cgggtgatct tcatcgcctc ctcaagcgtc tttcctttcg cgaggtcggt caccatgctg
    11401 ctcgtggcga tcgccgatcc gcacccgaac gtccggaacc ggatatcctc gatgacgttc
    11461 tctttgaccc tgatgaagat ctccatgagg tccccgcaga cagggttgcc gaccttgccg
    11521 tagccgtcgg gtggccggtc aggcgggcct cctcgatcgc cgtgatcccg gcgatgagcc
    11581 ggtcccggat cgcccgcagc cgcctgctct cctccggcat ctcctcccct gcaagccggg
    11641 ccgcctctcc catgcaggcg atcccgacga cgttctccgt cccgggccgg aggccgcgtt
    11701 cctgcccgcc cccgacaagg accggctgga cccggactcc cgggcggatg tagagcgcgc
    11761 cgaccccgtt cgggcccccg agctggtttc ccgagagcgt gagaaggtcg atgccgtcac
    11821 gctccacgtc gatcggtatc cttcccgcgg catcggtggc gtccacgtgg aggagtcctc
    11881 cccggtcgtg gacgatctcc gccgcctccc ggatcggctg aaccgtcccg atctcgttgt
    11941 tcgcgtagtg gaccgacgca acaacggtct ccggcgtgac cgccgcgccg agggcgtcga
    12001 gatcgaccgt gccgtaacgg tcgaccggag ccagtgtgag ggaaaacccg ctcttctgaa
    12061 ggtcttttgc cgggttcagc acggatatgt gctcgatggt aatcgccacg atccgcttcc
    12121 ctttctttgc gttccggagt gcggtccccc ggacggcgat gttgttcgac tcggtcgcgc
    12181 cgctggtgaa gatgatcgag gccggagcgc tcgcgccgat gagccccgct accttctccc
    12241 gcgctatggc aacggcctcc tgcgccgcga gcccttcacg gtagattgtg gaggggttgc
    12301 cggtgaaacc gtccggaaac tgcccggcaa accgtgcgac ctccgggtgg accggcatcg
    12361 ccgccgcgtg atcgagatag atccgttctt ccatattccg ccgtccctag aacgtgatga
    12421 ccgcgtcggc gtcgatcgcg aggtcgttca acgtgagcgc gcccacgacc tgcccttctt
    12481 cgatgaaatc accccgtgtc ataccgagga gctgggtgct ctgttcgcag atgtagatct
    12541 ttgcgcccgc agcaatcgcc tggttcctca tctcggagag tttcgggaag ttcccgacct
    12601 ggatctcctc cgctgcgccc ttcttgacga ccgtcacccc cttgatcatg aagtagatgt
    12661 cggcgtccat atccgtcgca cgtgccgtca tcgcgaggac gaacggtgca tacagccgct
    12721 ccggggtatc ggtgccgctg gtctggatgt acagtatccg tgccgtacct ttcacgcccc
    12781 tttcctgatt gagaaggtgg tcttctcccc ggccttctcc tcgcccacga tctcgtggcc
    12841 catccgtctt gcccagaccc tgacgtcctc cccggtgggg gcgttgtcca cgtcgacctt
    12901 caaaaccccg ccggccggca gccggtccat ctcttctttg agcatgatca ccggcgcctg
    12961 gcagtaggtc ccccgggcat ccacgtagcc gtcggcagcc atccgatcac ttcgcctcta
    13021 ccgctgccgc ccttcccttg cgcaggatga gggtcgtcac gcccgaggca acgtgctctt
    13081 cctcgatcgt atgcccgttc tcaccggccc aggacctgac cgtcactaca aggtctggtt
    13141 tgtcggtcgt cacctgcagg atctgcccct cgttgagcgc cttcatctct ccgtcgatcg
    13201 agagcatggg cgactggcag atgccaacac aatttaccgt cttatccgcg tacattcgtc
    13261 tcacctcact tcatcttcct gatggagaac ctcatgactc cgtcatcctt ctcgaacttc
    13321 acgatctcgt gccccacccg gttcgcccag cgccggatat cctcctccgc ggcagggtcg
    13381 tccgcctcga ccaggagcac ctctccgacg tcgagtctct cgatcgcctc tttcgtcatc
    13441 gcgatcggca tggggcagta gagcccgata cagtccacga ccgcagtggg tttcttctcc
    13501 agttccatct ctccaccttc cgttgcgggg cgcgcgtggg aatccgggca ccgtgacacg
    13561 tcaacggcac gctcccggct gcaccggatc gtgcaccatc ccatataagc gttcctccgg
    13621 gggtgcctgt ggtagagggc ggcaacggga ggctgttcgg gctccgggag aagatcagaa
    13681 gagttatact ccatctcctg ctactttcca tcatcgggga agaccgatca cctggagtat
    13741 gaccatggga ctgtggaacc ggtttggcgt gcgcaagcag gaggaggcag cggccgcatc
    13801 gtttgatgca gaagaggatg cccggatagc ggggcttgag aaattgctcg agagcgagga
    13861 tatcgggacc cggtggagag cggtcagggc gtttggggag atgggggaga cagcggtgct
    13921 cccgctgatg aaagggctcg ccgatgagga ctggtgtgtc cggagggtgg cggcaggcgc
    13981 cctcggccgg atcggcccgg cggcgatccc ccacctcatc ggcaccttct cgtacgcaga
    14041 cgacgctgtg cggcaggacg ctgtctgggc gctccggttg atcggaagcc cggcggtgga
    14101 cgccctgaag cagacggtgc atgacgggga tgcaacggtc cggtgtaacg ctctgcaggc
    14161 gcttgcggac ctccctgcag gcgatctctt cctggaggcg cttggcgacg acgacccccg
    14221 ggtgcggagg tgtgcgatcg ccggggtgaa gcggtcgggg gacgagcgtg gcatagaggg
    14281 tctggtcggg ctcctgcgtg atccggacga gcaggtcagg tctcttgccg ccgatgcgct
    14341 ctctgctttt gggggcgatg cggtatctcc ctgcatcgag gcgcttgggg acgacgacga
    14401 ggacctccgt cggcggggtg cggtggttct tgtccgtatc ggtgctccgg cggccggtcc
    14461 cctggcaact gctctccggg acgaacggcc gcccgtccgc cggtgggctg cgtgggtgct
    14521 cggcgaagtc cgggccggag aggcgatcgt gccgctcatc gagacgctct ccgaccccga
    14581 ccgcgaggtc aggtggactg cttccggtgc gctcgcagag atcggaacgc ccgccgtcgg
    14641 cccgctcgtc gaggctctcg gtgacggcga cgaggtcgcc cggcaccggg tgatggaagc
    14701 actctggcgg gtgggagagg cggcagcacc ggccctgatc gatcttgccg gtgccgatga
    14761 cgccgagacc cgccttcagt ccaccatcgt cctcggggag atcggtgccc ccggtgcttc
    14821 cggaactctg caggccctcc tcgacgactc cgacagagat gtccggcggg aggcgttcga
    14881 ggcgctggag acgatcaagg cacggggaga tctcgcctga ccgatgcgct ccaccgttgc
    14941 cccggtagcc tcgcgggagt tttccgatat cgccggtttt ggtaacgatt acggttcagg
    15001 ccgctctccg gggggagatc ggcctgttcg agcgcagccg cggccccgct ggtgcgcccg
    15061 ggcaatccgt agtttttcga gtattttccc ctttacccgc attttcctcc tctaatgtct
    15121 aaatgggtgg aaatttcgct ccttttttaa tttgtgactg ttattaccga aaattttaaa
    15181 aaactcaagc gataagaaga tccttaatgt tcgttccgac caagtgtttc tttacgaagg
    15241 gtgtaggcgt ccacaaagac aaactggcgt cgtttgaact ggctctcaga caggcgggca
    15301 tcgagaagta caatctggtt tacgtctcga gtatcttccc cccgaactgc cagctggtct
    15361 cgagggagga gggtttaaaa gacctctctc caggcagcat cgtctacgtt gtcatggccc
    15421 ggaacgagac caacgaacca agcaggctcg cctccgccgc catcggtcac gcgatgccga
    15481 aggagagcaa cgcctacggc tacctctccg agcaccatgc cttcggggag acggcggagg
    15541 tctcgggcga atacgccgaa gaccttgcgg caaccatgct tgcgacgacg ctcgggatcg
    15601 agttcgatcc cgaccaggcg tggcaggaac gggaacagat ctacaaggcc agcggccgga
    15661 tcatcaacac gacccacacc tgccaggcgg cggaaggggt tatggacggc cgctggacga
    15721 cggtcctcgc cgcagcagtc ttcctctaac ccacatctcc cggcagccat tcctttttgc
    15781 tccttgccgg ggccggccat atccggccgc cccccggcgc gctctcccgg gtgaggagcg
    15841 taaagcaaaa tacctcccgg gacccacact gtatccaggt ttttcatatg cgtataccaa
    15901 tacgggccgt aaccccggaa actgaatctg ccgagatcat cggctgggtg cacgaggtcc
    15961 gcgatctcgg aggacttgcg ttcttcctga tccgcgaccg gaccggcatc atccaggtga
    16021 ccatcccgaa gaagaaggtt ccggcagaga tccttgatac cgtccggagc gtctcaagag
    16081 agtctgtggt cagggtccag gggaaggtaa aggcgatcga gaaagccccc ggcgggcgcg
    16141 agatcgtccc cgaagaactc gagatcatca gcatcgcaga gagtccgctc ccgctcgacg
    16201 tcgccgagaa ggtgcccgcc gagatggata cgaggatcga ctcccggttc ctggacgtaa
    16261 ggaagccgcg tgtccgcgcc atcttcttca tccgctcggc ggtgacctgc gccgcgaccg
    16321 cgttcctctc ctcccggggc tgcatcaaca tagctacccc caagatcgtc gccgccgcca
    16381 ccgagggcgg tacggaactc ttcccgatcg cctactttga gaaagaggcg ttcatgaacc
    16441 agagccccca gctctacaaa cagatgatga tggctgccgg gttcgaggcg gtcttcgann
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcggggtcga cctcgccgtc ccggcaaagc
    16561 cgttcccccg gatcccgtat acggaggcga tcgagatcgc gaaccgcacc atcgaggaga
    16621 agctcgcctt cggcgacgat ctctccccgg cgggcgagcg ggcgatcggg gacgtgatcg
    16681 ggcaacacta cttcatcgtg gactggccga cggatatcag gccctactac gcgatgccct
    16741 acccggagag gcccgagttt tgcaaggcgt tcgacctgat gcacccccgg atggaactct
    16801 cctccggcgc ccagcgtatc cacgaccacg acgccctcgt ggagcggatc cgcgccaagg
    16861 gtctctcccc cgagagtttc gagttctact tgaaggcgtt ccgttacggc atgcccccgc
    16921 acgcaggatg ggggctcggc atcgagcgcc tggtgatgac gatgctcggc ctcgcaaaca
    16981 tccgcgaagc ggtgctcttc ccgcgtgacc ggcacagact gaccccatga ccctgacaag
    17041 catgtgtctc ccgaccaccg tggtcgggag ctacccggtg gtgaaaggct cggggcttct
    17101 cgcccgcatg gacccgctga agcacgcggt ggaggtagcg gtcgccgacc agatagccgc
    17161 cgggatcgat atcatctccg acggccaggt ccggggcgac atgatccacg ccttcacctc
    17221 ccacctcccc ggcatccgcg ggtctgcggt cgtcgggaag gtccagccgg ccgcacggcc
    17281 catcacgctc gcggatacga aatatgccct ctcccggcac ccgaaggtga aggggatcct
    17341 caccggcccg agcacgctcg cgcacggcct cgccctggag accccgtttt accggaacaa
    17401 ggacgaactg gttctggacc tcgcccaggc gctcgcggtc gaggcggact acctccagaa
    17461 tgccggcgtc accctcctcc agatcgatga gcccatcttc tccacagggg ctgcaaacat
    17521 agccatcggc cgcgaggcgg tgaacgcgat cgtcgggagg ctccgggtgc cggtatgcct
    17581 ccatgtctgc gggaaccttt tggatgtcgt ggacgacgtc ctccggacga atgttgccgt
    17641 attcgacttc gagttcgcga acaacccggg gaaccttgag gtgctctcgg gcaaggatct
    17701 gcggggacgg atgatcggct acgggtgcgt ggactcggcc gaccccggcg tcgagagcgt
    17761 cgagaccatc aaaaagcgga tagaagcagg gatagacgta ttttcacccg atgccatgct
    17821 catagacccc gactgcggcc tgcggatgca gtcgcgggaa gcggcgtctg gaaaactgaa
    17881 gaatatggtt gttgcagcag gactggtccg ggccgaatat acggactaga ccaccctgct
    17941 cgtcgtcgag agctcaaggc ccttcgcgat gatatggtag gggatcaccc gctgcggcac
    18001 gtttgcatct ttgagtttct caatggcgag cgtccgctcg agctcgttgc cgtggacctg
    18061 gtgcctgagg gtcatgtaga tgtcggcggc acgcatcatc cgcgcctcct cgagggggtt
    18121 gtggaggccg gtgtagagga ggtagacgat gtttgccccc ccttcgatga tcccgcgggt
    18181 gtcctcgagg acgaacctgg tggctgcgtc tattccccag gcggcgatca gcgtggagag
    18241 cgagtctacg acgatcctct caccctgcat ggccccggcc agcgccgcgg gatccatacc
    18301 ccgtgcgtcg accatcccct ccagcggcac ggcatcgagc atcaggtaag cacctgctcc
    18361 tcccgcccgc cagaactgct gtgagaggag ttcaaggccg ctcaagggag atcctgagat
    18421 gacgatgcgg gtgcccggtg gaaatcctcc gtcgagcgca agattcaggc ccgcgattcc
    18481 tgtcgaacgt ctggtggtgt ctgtcatgcc tatacttcca gggggtcctc aagggtatct
    18541 atgcgccgcc cttttcttaa tactacccgt caggtacagc cttatgcccc gcctgaagca
    18601 cagtactctt cccggaggcg cttccgcatg agtttccacc cgttcacccg ggggatctcg
    18661 ccggcaaaga tgacccggcg ggggaccttg tagcccgcaa gccgctcgcg ggcgaaggcg
    18721 atgatatccc cgggtgtgac gccctcgccg gaagggacca ccacggccac cggcacctcg
    18781 ccccggtgct cgtcggtgca cccgaagacc gccgcgtcgc ggacacccgg gtgctggacg
    18841 agcacatcct cgacctccgt cgggtagacc ttccagcccg acatgacgat catgtccttc
    18901 ttccggtcgg tgatatagag catcccggtc tcatccaggt ggccgacgtc gccggtcagg
    18961 aaccacccgt cggggaggaa ggcggcgccg gtctcctccg gcatctgcca gtagccgagc
    19021 gccaccgccg gcccccggag ggcgatctcg ccgtcctcac cgggtccaag ttcccggccg
    19081 gggtcgtctg cggcgacgat cttcacctcc gagaatccga ccggagtgcc gacgctctta
    19141 aactcgtcgg cggtcgcgta gtgctcgggg cggatcgcgg tcccggtccc gacgacgacc
    19201 gtctcggaga ggccgtaggc gttgacgatc gggataccga accgctcgtg gaagggtttc
    19261 cagacgccgg gcgtcagggg cccgccgccg ctgatcatca cccgggcggt ggcgagcgcc
    19321 gcttcagtcc ccggcggggt ctggaggagg gagtggatca ccggcggcat gccggcgacg
    19381 atcgtggccc ggtagtcacg ggcgagatcg aggtaacggg cgagatcgaa ccgctccatg
    19441 acgacgtaag tcccgccggc ccggagcgtg gcgatgcccc agctgatgcc gacgtgcccc
    19501 atcgggtaga tcccgaggta gacgtcgctc tcggtgatcc cgagaacgtc ccgctcggcg
    19561 tccatggccg ctatccagtt cccgtgggtg agcatggcac ccttcggttt cccggtggtg
    19621 ccggaggtgt actggatctg gcagaggtcg tcgaaccggc agtgggcggc gcggaggtcc
    19681 ggagacgcac ggaggaggtc cgaccagggg gtggcgcccg cgatcgctgt cccgacggcg
    19741 acgacctgcc tgagcgtcgg tgcccggtcc cggatgcggc cgacgagggc cgcaccctcc
    19801 gcatcggtga tgacggcggc agcccctgcg tcgcggacgg cgtagaggac ctcgctctcc
    19861 cggtagaccc ggttggtcgg gacggcgacg gcgccgatac gccagagggc gaagtaactg
    19921 atgagatatt ccgccgaact gtcgagatag atgcagaccc gctccccgcg agtgatcccg
    19981 agagcgagga ggccgcgtcc gacccgacag acccggtcga gaagttcggc gcgggtgtag
    20041 gactctccgg tggcggggac gacgagagcg actttttggg gattacaggc gttggcgtcg
    20101 agaaatgtgg tgcagttcgg catgggggga ctccgcgcaa caggtatgcg ctttgatgta
    20161 tatgcttgtt catagtgctg taaataggtt gccgcggggt tacgggagtg atccacagcc
    20221 gcgaccccgg cgggtaaaac caaaggaaga ttaccggaca gggagaaccg gggtgtatgc
    20281 agagcgacac gccgctcgta cgcgaggccg tccgggagga cctggacgcc gtcctcttgc
    20341 tctacacgca catgcatgat gccgatgagg aggccgatgc tcgtgcactg gagacggcct
    20401 gggaggatat cctcgccgac ccccggaccc atctcttcct cctcgaatcc ggaggcgtgc
    20461 cggtcgcgac gtgcgtctta cacatcctcc agaacctcac gcggggcgca cggccgtacg
    20521 ggctcatcga gaacgtcgtg acgcatcgtg agttccggaa ccggggattc gggacggccc
    20581 tcctcaccca tgcgctgaag tgcgcgtgga aagagnnnnn nnnnnnnnnn ngtcaaagaa
    20641 gggctgatcg cgtatccgcc ggagacccgg tgaaacggat cggctaaaaa gagtcctccg
    20701 gggtatcccc cggagagccg cttactggta ttccggcggc tgcacttctt tgggcagccc
    20761 gcccttgaag tgctgctgca ccttcccgta gtactgccgc agccgctcgt tcatcgtcgc
    20821 gtgcaccttc tcctgcgccc ggtcgaagtg tgacttattt acgagggagg ctccctcccg
    20881 catggcaagc atggccgctt cccggccgaa cgcctcgagg tccgagccga cgaacccctc
    20941 ggcatcggtc gccagctttg caaggagccg gtcccgtgcg gggtcgtcca gggtcacctt
    21001 ctgctggata aagaggtcga cgagcttcct cctccggagg tggggccgga gcccctcgtt
    21061 gtcgctcggc ggaattgcag cgcgatggct cttcacgtcc tcgacgctca cctggcggtt
    21121 ggccccgacg gccaggatca ggtcttcgag ttcgttctcc gagagcccct ccgtcagctc
    21181 cacgaggtct tccaccgtcg agccctcgat cggcatatac cgggtgtgga tagccaggat
    21241 cttctcccgg tcgtcccgcc cgggttcgcc tatgtagacg agccggtcga accgcccggg
    21301 ccgaagcagc gccgggtcga ccatatccgg ccggttcgtc gctcccatca ccaccacgcc
    21361 ccggagttcc tcgagaccgt cgatctcggt gaggatctgg tttaagacgc tctcgatcac
    21421 gtgcgactcg ctgccgccgc cccgtgcggg ggcgagagca tccagttcgt cgaagaagat
    21481 gatggccggc gccacctgcc gggctttctt gaagatctcg cggaccgccc gctcgctttc
    21541 gcccacccac ttcgagagga gctgggggcc cttgaccggc acgaagttcg cgccgctctc
    21601 gcttgcaatc gcctttgcga tcagggtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnntcgag ggtctcgggc gggatctcct cggcctccag gtcgatctcg gggaggtaac
    21781 gccgcagtgc cttgatcgcc gcctcacgtg cgagggcggc gaggtccgcc ccgacaaacc
    21841 cgtgggtctg ctgggcgatg gaggcgatcg agacatcgtc agcaagcggc ataccacggg
    21901 tgtggatatg gagcacctgg atccggtcgt cctccgacgg gaccccgatc tcgatctccc
    21961 ggtcgaaccg tcccggacgc cggagggcgg ggtcgatggc gtcgagccgg ttcgtggccc
    22021 cgatcaccac gacctgcccc cgctcttcaa gcccgtccat catggtgagg agctgggcca
    22081 ccacgcgccg ttcgacctcc ccggtcacct cctcgcgccg gggggcgatg gagtcgagtt
    22141 cgtcgatgaa gatgatggcg ggagcgtgct gccgtgcgtc ctcaaagacc tcacggagcc
    22201 gctgctcgct ctcaccgtag tacttcgaga tcacctccgg ccctgctatg gagatgaaat
    22261 gcgctccgct ctcgcttgcg accgcctttg cgatcagggt ctttccggtc cccggcggac
    22321 cgtagaggag gacacccttc ggcggctcga taccgagctt cctaaagatc tccgggtggc
    22381 gcatgggcag ttcgatcgtc tcgcggacac gctgcagctc gcctttcagg ccgccgatgt
    22441 cctcgtagct gatcctcttt acgccctcaa acccggccgc aggcttctcg gagaactcga
    22501 tcttcgtgtt cttggtgatg atcacggcgg tctccggctc gaccaccacg gctttgaacg
    22561 cgaccagctg gggctgcatg aacgggagcc ctgcctggat cggcacggag tcgtttttga
    22621 cgaccggaaa gtcgatcagt ttgttgacga tgctgccgta gttgatgggg agctgcttgg
    22681 ggaggtcctc gggaggcgcg agcacgaccc gtttcgcctc gtcctccgca tccagcgttt
    22741 ttatcttcac ccgatccccg atgctggcac cggtgttgag ccgggtgaag ttatcgatcc
    22801 ggaccttacc ctggtgccag tcgttcacca tggcacgcca gaccttggcc acggtccgac
    22861 gcttcccttc gatagcgacg aggtctccgg ggttgagccg aagttgcagc atggtatcag
    22921 ggtcaagccg tgccttgccc gccccctgat cctccggata agcgctatct accttcaagt
    22981 atatctcggg cattacctct agttcttata tttcggggat ttataagtac tggagacgca
    23041 tgaacatact tctcttcgac ccgttcaacg gagccgcagg cgacatgatc atcggaactc
    23101 tgctcgatct gggagccgat gaagaacctg tcaggacagc catgcgttcc gtcgtcggcg
    23161 aaccgacaat agaacgggta gaccgctccg gtattcgtgc ggtccaggtg aaaacgcatg
    23221 ccccggtgga tcaccgcact cttgacgagg tgctcgaccg ggtggccggg agcgacgccc
    23281 ccgcccccgc acgggatatg gcacggcggg tctttctccg gatacaccgg gccgaggaga
    23341 gcatccacgg gcccggagcg cacttccacg aggtgggtgc ggacgacgcc attgccgacg
    23401 tggtcggggc ctgcaccgcc ctccacgccc tcggcgtcga cggcgtggcc gtcctcccgg
    23461 tcgccctcgg ccgtgggttt gcggtcgggg ctcacgggac cttccccatc cccgccccgg
    23521 cgacggtggc gatcctcgcc gcatcagacc tgcagaccgt ctccggtaac gaggagcggg
    23581 aactctgcac cccgacggga gccgcactcc tcgccgagtt ctccacggtc cggtccgaag
    23641 acctcggctc tcgcaccatc cgggcggtcg gctacggggc cgggagcagg aacccggcgg
    23701 gaagacccaa cgtcctccgg tcgatgctcg tcgcgacggc ggagggggcc accggggacc
    23761 gggtcgatat cctcgagacg aacgtcgacg acgtcaccgg ggaggccctc gcctacacga
    23821 tcggctgcct gatggaggag ggggcacggg acgcaagcgc cattcctctc gtgatgaaga
    23881 aagggcggag cgggcacctc gtccgggtgg tatgccgccc cgatgcgacc gacagcctcg
    23941 tgggcgtgat ggcccgggag ctcgggacgc tcgggatacg gtgcataccg atggttcacc
    24001 ggtcggtcgc cgaacggacg gttgaagcgg tcacggtggc catacgtggc cgggagtgga
    24061 tgatcgacgt gaagtgcggc tggtcggggg agaagatcac ctccttcaag gcggaatacg
    24121 agcaggcggc ggcgtgtgcc cgggagaccg gggtgccgtt ccgtgaggtc gccggcgccg
    24181 tcgaagcggc tgcacgccgc ctcttcttcg agcggggtgt gctcgcacca tgaagccgga
    24241 ccgggtcagc acggggagcc agaccctcga cgacctgctc gggggcgggc tcgagcggcg
    24301 gacgatcacc cagatctacg gcgagtccgc gagcggcaag agcgccttct gcctgatggc
    24361 cgcggtggcc tctctccggg ccgggaactc ggtcgtctac atcgacaccg aggggttctc
    24421 ggtggagcgg tttacgcaga tcgccggggc ggacgccgca gagttcgcag accggctcta
    24481 cctcttcgag ccgctcgact tcgcccagca ggggacgatg atcgccgata cggaagggct
    24541 gctcaaggcg agcgaccatg cctccatcgg actgctggtg atggactcgg ccaccgccct
    24601 ctaccggacg gaactcgatc tcgggcggga ggcgttgcgg aagctcctgc accacatgat
    24661 aaaactcctc gggcttgcga agaagtacga catcccggtc ctgatcacca accagatctt
    24721 catggatatc gaacgcgaac gggtctccgg cctcgggggg acggcgctcg agcacctctc
    24781 caaggccatc gtgaggctcg aaaagaagga tagcgtccgg cgggccatgc tcaggaagca
    24841 ccggtcccgg ccggaagggc tcacgtttga ttttctgatc accgaggatg ggatcagaaa
    24901 agtatagtgc cctcaaagac tgtttcggcg gggccttcca tggtgacgtc ctcgccgaag
    24961 tggatgacga gcggcccgcc ttttgtctcg actcttactg tctccccggc gttgccgagg
    25021 tggcgggcga ccgctgccgc cgcggtggcg ccggtgccgc agctctcggt ctcggcctcg
    25081 acaccgcgct cgaacgtccg gatccggagc acatcaccgg cggtctcgac gaagttgacg
    25141 ttcgcaccct caaggaagga gggatggttc cgtatcgcgg ggccggcggt ggcgatgtcg
    25201 acggcgccta tctctttgac gaagacgacg gcgtgaggga cgccggtgtt cgcggcgtag
    25261 acggtgaagt tgcggatctc ctctttgtat tcgccgttcc cggcggcggg gatggcgctc
    25321 cgctcgaatt tcggcgccgg catcgtgatc gtcgcggtga actcgtcgtc cgcgtagccc
    25381 atcgtcaccg ccacgatccc ggccccggtc tcgaccgagc aggactcttt gacgtagccg
    25441 gcgtcatggg cgtacttcgc gaggcaccgg atgccgtttc cgcacatcgc ggcctcgctc
    25501 tcgtcgggct ggaagagccg catccggata tcggccgcat cggatgcctg gaggaagagg
    25561 atgccgtcgg cgccgatgcc gaacctgcgg tcgcagtaga gcgccgcaaa cgctcccttc
    25621 atctcctcgg ggatggcttc ctgttcgaat tcgtcaatca gaataaagtc gttgccgttt
    25681 ccctgcagtt tggtaaatcc gatctccatc tccatagtgt agcctcnngt attgggagcg
    25741 gcgggtgata aaccgtggtg gttgggtgga ggatggagga tattcggata cggtccggca
    25801 tggagcggtg gttcgggagg cgatcggcgt tcgtgtcgtt cctgcccgat agtatgggcg
    25861 ggaccgatgg gatgggtccc tgcctttttg cagtcaccgg ggctcttccg ggtagttatg
    25921 ccggttcacc aggagggcat acccgccgtc ggcggtctgg acaaccgaga ccgggttgcc
    25981 gatcagttcg aacgtggcat tcagttcggt gtcccactcc gggagtccat cagcgttgag
    26041 tttcatgatg tgaggaacac ccacctggtc ggggaggatc aggaaccggg atgcgggatg
    26101 gtacgtaacg gtaccggata cgtaccctcc atcggccgtt ttgatgatcg gtgccgatac
    26161 gttgagcgtc ttctctccga tcacgttccc ttcccggtcg aacacgatct cctccagcgt
    26221 tacgttgttg tagacaacgg tgcactcgcc tcccggcgct tcgctcatga agactacatg
    26281 gttgtactgc gacggactgc cgagcgttgc ctcccagagc agatcgcctg tggcattcag
    26341 tcgaagcagc cacacgtcag agccgtttcg gttcggaaga taggttgtac cgcccaccag
    26401 gtacccgccg tccgaggtct ggatgacggc cctcgcgctg tcccggttgc cggtcccata
    26461 gatcttgcgc catttttccg tcaggtttcg atcgctcttg agcaccagga gatcttcgcc
    26521 ggcagccccg gcgatggcaa ccccgccatc ggcggtcccg acgaccgact gcaggatgcc
    26581 atcgacctcg acgtcgctat ccaggatgtt ccccgcagga tcgaagagca ggacgccaga
    26641 ctcacccacc gctacgacgg tgcccccggc cgtctcggcg acggaagaga tcggggctcc
    26701 cctgctggtg gtgttccaga cgacggaacc gttgccgtcg agtttgaaga ccccgtagga
    26761 ctgttccccg cgccagggcc ggacttctcc cccgataagg tacccgccgt ctgccgtctg
    26821 gaccatgcac gctccggtat cgtcgccccc ggtatcgatg accgtctgcc actgctccgt
    26881 tcctccggcg tcgactttga agaccatgat gtcacggccc accccgtact ccggcacaag
    26941 gcaccctgcc gtgaggacca gcagaagaca gagcgcagtc gccaggatac cgattccccg
    27001 ggatgcagtc tgtttcattc tcctattccc cctctccact cgagctgcgc attaagatgc
    27061 ccgcaatcag gcagatgaac gctccgacga ggaacacctc caggtacagc cctgccagca
    27121 acccgaaaga gaagaaaccc tcatacccgg gctccccggc tgttccgatc gcttctgcaa
    27181 gagcatcgaa gagtatgccg agaatgggca gcctgtcagc gaaaagcggc ggaggggcgc
    27241 ggggcaatct atcaggattc acgagcaggg caaacaggaa cggcacctga atcagtgacc
    27301 cgactgcaat acacaatttc ctgccgcccg tgacaaagtt ccggaacaga aagtatgccg
    27361 tcgccaccgc agatgccgag atcgtagccg tcgacagcag tattatcccg gatataagcg
    27421 ggctggatgg agagcctgcg tagattgtac cgattgtgcc ggataccgcc gcaacgattc
    27481 ctatcccaag cgagacctcc agacactgct tctcttttcc cagattcccg atcagggtta
    27541 ttaaaacgga cggccacacg aggacaaagg cgacagccag aaagaacatc agcggtgcaa
    27601 taatgtttat cgcattgttg ggatacatgt ctgcgtactc ctagaagaaa ttcggtatcg
    27661 attgaaggat ggtgctgtca cccgaaagga caaaaaatga tgcaggaatc acattcggcg
    27721 ctctcaatat aaatatcaaa attccacatg caaagcaggt tctggagaga gcacgatcaa
    27781 atctcgaaag ataacttccg caaccaataa aggcgagccc taagccgccg attatcaagg
    27841 gaacaatata cgttatctcg catagaagcg cgattgagcc taatagaatc gaattaagca
    27901 tggaatatag cacagcataa ctgatgaaag ggacagttgc actgagccaa aagcccatgc
    27961 gtccgaaaaa tgtgtatgta agccctattg gaataatata attaaacact atgccaaacc
    28021 cgagaattaa ccttaaaggc atcatctcaa cttgatgcgg aaaaactaat gttatcatat
    28081 gcatcatcaa aagagacaat agaagacccc cgcagggtgc aattaagcaa cccaaacggc
    28141 taaatgaagg acaactaaat gaatggatcc ctcgcaatcc tataatttgt ttcatttgaa
    28201 tactccaaaa cagggggtta atccatgtca aatatatacc cccatccatc aaagatgcca
    28261 gatggatttt gcaagtatat ccacgaagcc gatgcatcgc cagataatgc gccgtacagg
    28321 tggtccctgc caacattcga tgaatccggg tagaggtaat gcgtcaagcc gtcccatgac
    28381 caatattcgt agtgacagtt accgtagcac cagtcatcac caatgttcgg agaataatga
    28441 ccatcgacca taacaagatg ataccgatcg ccgtaataac tgccgtcctc aagttgatga
    28501 tttcctagcg gattgctccc agggctattc gtccagacca tgttgtccag attcggtcca
    28561 tgtaatcccc attcagtcca accaagtgca ctgccccaac cattgctttt gaggaacgat
    28621 gccatttcgt ctacagtttt gtcaaagaaa atcgcgttga tgccctcgcc gtttgaggtg
    28681 tctgtgtctg tgttaagata cggccacaga tacatatcgt acaaaccaat ccgcacaatt
    28741 gttgcatctg atctcattgc tccggtagtg agcgctgtgt atgcatcatc gatcagttgc
    28801 tgggttttat catcgccaac ttgttcacga agaaacttaa tgtacccggc gtttgcctct
    28861 tggaattcct ctttcgtcat tggtctatca tacgtagcag aatctatgga gtaagccttt
    28921 gactccatga tgggaaggga tatgtcggca atttttacta acttttcatc tcctatttcg
    28981 tattcgctac ccgattcgag ttcaagactc tgtgcactca ctgcaggcac caccacgccc
    29041 accagcatta ctgctaagag cgcgaagagt gcccgcattc caatttttcc attcatttca
    29101 tttccctccg ataactcccg gaggtaggag aggcatacct tcaagtacgg gaagagcata
    29161 cgttcccttt gcctccgggc gcgcgggaca ttcacccctc caattcgcta ctggccgggt
    29221 ggacttccgc actgttgcag gcgccgcctg cggcacctac aagtgttcac ttgtcagttt
    29281 tgagataaac tgtttctggg acaaacacca attcgaaacc ggctcttctg gaaaagagca
    29341 gtcctaacgc cctgggatca ccgtaatggt atcctctgcg gttgaccggt cccgaaggaa
    29401 ttccctcccg atggcaaaag tgtcgccggg atgagtaaga acgctccagc agatccggtg
    29461 ccggaacctt ctcatcggtc actgcgcgca ggtcgcccgg tgcgtccatg ctgtacgtgc
    29521 catcggagca tgggccgggg tgcaaccgca cggcgaagcc tccttcacgg ccacgtcgtc
    29581 accactacgt aatacgcatc ttcgtactcc atgagcggat acagtcccct ctccccctgg
    29641 gggccgtaag catcgtggag cgccttgctc tccctgtacg agaccttagt tcccccgacg
    29701 acgcgctggt cccgcgaacc ccactcccac gccgacgggt tccgcccctc gccccggatg
    29761 accgcatcga gcgccggatg attggcaaaa tccttctcgg tcaggaggat gaccatgctc
    29821 tctcccggct ccgctctctc catgacgtag agcatcggct gcccgcccga actctcgtgg
    29881 gcgtagagga taatggtgtc ggcgagtatg aagccgagcc cagcggcgac gacgcctata
    29941 aggccgcata cgatgataaa ccctgccctt ttgagtcggt ctctggattt catttgtgta
    30001 tcttctgcca tgtggtctca cgacctaggg tatccagatg agaaagtagt aatacgcacc
    30061 gtcatactca aggtatgggt ggtttcttgc ctcgacatcg gggccgaacg actcgatgag
    30121 tattccccgc tccacccacg taaccggagc gcttccaatc gttcgcttgt cgagggcacc
    30181 gtatggcgtg tctcccgaat accaggctcc ggggtcccgt ctgtcgcccc ggagcacgga
    30241 gtcaagtacc ggatgctgct caaaatcttt ctcggtcagg tggatgatcg atgcgttctg
    30301 cagaggttct cctctgatct cccaggcgtt caatcccgga gacccgctgc ttcccgcgat
    30361 ccaggaagag agggcggtat acgcgagcac aaccgttgcg acaaggagga tgacgccgat
    30421 gaacgtcaga accccgatgg cgagccgccg tctgttgact ttcgatttca tgatctcctt
    30481 tatgcttttt tcacccgatc ccgctgaagc gggagggatt ccattcgtct cttccagtgc
    30541 attctgtttt tgattcctgt atcattaaaa gatctccagg cgataatccc aaaaaatgaa
    30601 ttaaaggtga gattcaaacc cgatcatatc aggagtgata gttcgcaacc gtccattttg
    30661 tctcccggat tctccttgcg ttgtcgtgat cggcatcccc gtggtagtcg tccgatgaaa
    30721 attcagtggt gctcagtagc tcattgaagt aactgtatgt cacggtattc ttgaggttga
    30781 ggggaggaga taacggttga taccactggt aggctctccg ataggatggg tttggccgtt
    30841 cagaatccgg atcctggtga ccggtatcaa agaggtgccc cagttcatgg atactcacac
    30901 atctgcggcc gtgtgtggta cccaggtatc cgaggtcttc gccgaccatc tgggcccagg
    30961 catgccggcg atggtacgga tggtaaccat aagtagctcc ctgcacagaa cccgctgcat
    31021 catatcctcc aacatatagc gcaatatccg aggcccagag atcaaggtcg ctgttcggat
    31081 aaaccctctc aaacgcatca acgggttcag tcaggatatc gtgctgattt gagagttgaa
    31141 cccgcctgga atcagtatat gccattacac agagcgacac ctggatatcg tcccggccaa
    31201 actgccggtt cgcctcggct acgatatcct gagcgacaga tttccagttg ctttggtcct
    31261 ggtagaactg gttgtctgta accaccaaca gattgaccgt tgcatactga tcccggagtc
    31321 ctatcatgga agattccaga tcttcagaga catcggtcga agtgtatccc tccgggggtg
    31381 tgagcgtccc gtagtcgata acaactgatt tttcggggtt ttcaatatcc cgagaactgt
    31441 agatgacatg cagaggcgac gcttctttct ctgcgcgcgc gcgcgactcg actggggtga
    31501 tccagaacgt ctcatcaccg agtgtcacac tcccgacgag gacgttttcc ccggtcgtca
    31561 ggagaacatc gctcccatca actccggcga tcgttccctc atatgaatca ataccgtcgt
    31621 tgatctgctc gaaatccatc ctgtgcagtt cggcctggta ctccttgccg tggatctgga
    31681 gagggagtac cccgcggacc tgtttgttca gtgcagcatg gtcgaacgtc acgaggtcgt
    31741 accgggtaat gccctttggg atattcagtt cttccggcgc ggtgattccg gatgctcgca
    31801 tatcggatac cgatgtagca cccgcaacca gcacatccat agagtcaccg atgactaagc
    31861 ctggctcgcc gctgcccgcg gtaggggttc ctgttacgtc ctgagcgctc accgcaggca
    31921 caacacccac gctcaccagc agcagtgcca ggagcatgga aagtgcccgc attccaattt
    31981 ttccattcat tttacattcc tccaggggtt tcccggagac cggagcggca taccttcaag
    32041 tacgggaaga gcacacgttc cctttgcctc cgggcgcgcg ggacgttcac ccgacgattg
    32101 gcaaaccggc tgggtgaacc tccgcactgg tgcaggcgcc gcctgcggca cctacaagtg
    32161 ttcacttgtc agttttgaga taaactgttt ctgggacaaa caccaattcg aaatctgact
    32221 tttctggaaa agagcagggc taacgccccg ggatcactct agacaccccg cctgcaacgg
    32281 acctgtcccg ggagaactcc cgtccaatga caaaaacagc gccgaaatga gcagagatgt
    32341 tccagcaggt ccggtgcagg ggcgctctcg ccgaacactg cgcgcaggtc actttctctg
    32401 agattttggg cagcactgat gagtagagcc cgtcaggacg acaggggggt gtatttatgg
    32461 caccccccgg aaacaaaaga ttcgttccgc cctattctgc catgcgcctg ctcaggtaca
    32521 acggggccgc ggacagcaga atcaacccgg agaccaggta acgaataatc tcacgtgcag
    32581 ggcattccac agaccgaagg tagcaggaga ggtcgaaggc aaaaaggggg acggcgatga
    32641 tcaggccgat cttccagaac catccaacga tgctgcaatc gccaccatct catccgtctc
    32701 ctgtgtgccg gaaaggcagt atgcatagcg gctgtcattc cagcgaagca cgttttcccc
    32761 gtctccggga gtaaacgttc cgtttgtccc gtcgatcgtc accccaacag aacctgtggt
    32821 tttctgcccg gggcaggggt tgcccgggac aggcacctgc gtgatacgaa gttcatcctc
    32881 ccctttcgtg tagatcaagg atattcggtt gtccggttcg ccatactgta atgcatacga
    32941 gaaagagtag ccatccggca gatacgatgg taccgggaga tccgtgccaa acttttcatg
    33001 cgcctctttt agcgtataca cgatgacggg cgttatcgca agtgacggag cggttggatc
    33061 ggttgtaggg ccgataatag atccgttgct gccggaatcg gatttttgag gctcttcccc
    33121 gtaatcgagg catcctgcag caaccaatcc cacaacaagt agtacaaagg ttactagagt
    33181 aagcattttt gggttcatgt caactcctct tgcagttact ggaattctcc atgcgtatcc
    33241 agagttttgc atatcttact cacagaaata tttcgtgagg cgattgaagc ctcacttcgc
    33301 tggtgcatcc acacatgtat ggtatgaata aattttatcc gatgataacc acgtgctaac
    33361 gtgtacgtag ggctggtcat cagagtatct gataaaatca aatgtctgcc ctggtgaata
    33421 ccaagtacca tcacttctcc gtgtccactc atcgaggatg ggataggcaa ttgcttcgaa
    33481 gtagtgttga acacctccat atatgtataa ctcggatgat cccattagcc aatcaactgc
    33541 agtggcagga ttgtcagtgt catcgtagga atagtagtac cattgatcag tgctttggtc
    33601 ctggaaccaa atctcataca tgccgttggc aatactaaca tagtagtcaa agtagggctt
    33661 ggcagttgca tcaatccacg tacttgttgt ttcccacgtg agattgtgct catcatatat
    33721 gggaaccgaa aggaagatct ctccatcatt tctgtagaac agggtaagct cgattatatc
    33781 tccatccctg tttgtataaa tctctctttc aagatagaca actgcatcct gcccttc
//
LOCUS       DAJB01000092           12596 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_55397, whole genome shotgun sequence.
ACCESSION   DAJB01000092 DAJB01000000
VERSION     DAJB01000092.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12596)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_55397"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2787 a         3771 c         3717 g         2321 t
ORIGIN      
        1 ttccttatca tggtgggggt agatcaggct caaatagata aaatactctc cggcgcgtcg
       61 tccgaggacc cggaggcccg tctcgatacc ctcgagaagg aactggactt cgtcaagacc
      121 tcgatcaagc gcctgctcat cgacctccgg gaacgaatga acgaactgga caacccgttc
      181 accagcaccg ccgtatcctg cgcagggagc gagttcaggc cgcgggctgt cgcctcgaac
      241 agtgaagatg cagacgatga gatgcccgcg ctcgatccgc cctctgtcgg tcttgactta
      301 aaaccggaga aagcagtgcc ggtgggcctg gacgccggac tcttcccggt cgacctcaga
      361 gcggcctgcc ttcccgcgga cagcatggct gctctgcccg aggcaaagcc cgcgggaaaa
      421 ctcaaactcc agaaggtcca ccagctcttc ggatgggtgc accaggggtg ctccaggtac
      481 gggcacgagc acatggcaat catgatcgat gcctaccggt ccatgggcta catcaacgag
      541 gaggtctccg accagatcca cgagatcatg cggatggccc ccgagacccg gggcgacgtc
      601 cgggatatcg gcccaaacga gttcgtctcg gagctctacc tcctgaaccg gatcctcgac
      661 cccgaagacg cgacgctcga ccgggacatg atcgaggtgc tgatgctctc ccggcgcagt
      721 tccggggctc aggacgccgg gaaaacctcc tcacaggaac gggacgccgg cgatacctgg
      781 attgagttgc tggacaggat atgatggatg tcgagcgaaa ccttcaccac agcgatgttc
      841 cttatcgccg ccatcatatc ggccggcgtc ctcattaacg cagtatttcc cgtggtctac
      901 acgctctcgg gtacgatttc gtcgtcctcc cacaaggtgg acgaacggtt gagcacggac
      961 gtgaagatcg tcacgacgta cgccagcggc gggggcaata atgcccgtat ctggctgaaa
     1021 aacgtcggcg ccggcaggat tgccgatgcc agcatacaga aggccgacgt cttcctcggg
     1081 agccagggcg acttcatccg gttaacgcga tcggagacgc tcgcggacgg gacctggacc
     1141 tacgagatcc tggaggagac cccgaacaac tactgggact ccggcgagac cctctacatc
     1201 gaggcgatga ccacaaagat ccccgccaag gaggggatcg tctacttcca gttcgtcctc
     1261 cctacaggac tctaccggtc gaccaccttc accgcgagtg attaaccatg agtgcaggac
     1321 ccctcatcgc atccggaatc ggcatcctcc tcctggtcac cgtcgcctac atcctgatcg
     1381 gcggcaccct cgtcaccacg gaagtgatgg tcgaagcgca gagcaacctc gccacctacc
     1441 aggaggcccg gatgcggacg gcgatcgcgg tccagaacac gtcgatcgcc gactccaccc
     1501 tttttgtcga ggtgaagaac accgggagcg aaccggtcgt cggggtcccg tccatcgacg
     1561 tctacctcca gagcgagggc gttccggtct atgtcccctt cgggaactgg agtaaggtga
     1621 gcataacccc cgacgaggtc caccccggcg agctcgaccc cggcgaaacc ctcaacctct
     1681 ccgtcacgta cgatatcatc cccacatggg ttcaggtcgt caccccaaac ggggtctccg
     1741 gttcggcata cataacgggt ggataggaat ggccagaagc aaagaagcag acagcagtat
     1801 tctccagacg cccgacagga cgatcctctc gaccgggaac tccgaactcg acaagaagct
     1861 cgccgacggc ctcccgcttt cgtcgctcaa cctcatcgag ggtgagaacg acaccggaaa
     1921 gagcgtgctc acccagcagc tcatctgggg ggccttaaag caggggttca acgtcgacct
     1981 cttctcgacc gagaacacga gcaagagttt cctctcccag atggagtcga tgagcctcga
     2041 catctccgac tacttcgcct ggggttacct ccgggtcttt ccgatgcacg tcgtcgggtt
     2101 cgagtggaca aaagagaaga tgcaggggac gctcgagcgg atgatccact acatggagca
     2161 gagcagagca gacgtgatcg tcatcgactc gctcaccctc ttcaccgagt acgccaagca
     2221 ggacacgatc ctcaccttct tcacgaactg caaaaacctc gtcgaccacg gcaagaccat
     2281 cctgatcacc ctgcacacct acgccttcgt cgaggactcc cttgtccgta tccgctcaat
     2341 ctgcgacgcc catctcttca tgaagaaagc gctcgtcggc ggcaagtacg tgatgatgct
     2401 cgaggtcgtc aaggtccgcg gcgcacgaaa gaccaccggg aacatcatca gtttcgaggt
     2461 ccaccccggg tacggcatca agatcatccc ggtctcaatc gcgaaggtgt gagagatggg
     2521 atcggcgctg gaagcaacgg taaccctccc cttcgagccc gagttcatcg acgagggaaa
     2581 cgactgctac aacaatgtag agtcctgcgc cctctaccgg atgctcccgg caaacgcccg
     2641 ggactacgtc aaagcgagcc ctcacctcct cgagtacctc catatcctgc cggtcaacac
     2701 cgtcggcatc ccgctcttcc tctcggagct gaagcgggac ttaaaatcga tggagaaccc
     2761 gaacgtcatc tacccggtga acgacacgac gttcgtccac atcttcccgg accaaaacga
     2821 cgtccgcaac tggtacatcc cgatcgagcc gtcgttcctt cactccgtaa aagcaatcct
     2881 cccggtgatc gaggagaaac tgatcgatat gatcgacgtc ctcgacgagg agccggtgac
     2941 cgagaaggcg cgtatcgagg tgctcagaaa ctttatccag cagctggtat acgtccagct
     3001 gccgggcgaa gagaccgaca agtccctgat tgccggtggg atggaaaaag actggaagag
     3061 ccggatcaag accttcctca cctccgaggt cggtgcgtcg gcaaagccgc aggcgtcagg
     3121 gcatcccacg cttgccgacg gccgtatcat cctctcgcag caggagtaca aggcgctcga
     3181 gtacatgata atccgcgaca agatcgagat ggggaccctc aaaccgttcc tctccgacca
     3241 gtatatcgag gatatcacct gcgacggcgt cgggccgatc ttcatcgagc acaagatctt
     3301 caaaggcttg aagtcggtcg tcgggttcca ggactcaaaa gagctcgata ccttcgtcat
     3361 caaactcgcc gagcggatca agcggccgct gacctacaga aaccccatcg tggacgcgac
     3421 cctccccgac ggctcgcgta tcaacatcgt ctacgggacc gagatcagca agcacgggag
     3481 caacttcacc atccgtaagg tgaacgaggt ccccctgagc attctgcagg ttatcgagag
     3541 cggggcatgc aactacatga tggccgccta cctctgggtc tgcctcgagt acgggatgtc
     3601 gatgttcgtc tccggcgaga ccgcgagcgg caagacgacg accttaaacg ccctgacgac
     3661 cttcctcccg cccgagaaca agattgtcac catcgaggac accccggagc tgacggtccc
     3721 tcacaggaac tggacccgtg aagtcgcgaa agccaagggg aagggcgagg gagaaggctc
     3781 ggagatcacg atgttcgacc tcctcaaagc cgccctccgt cagcgcccga accagatcct
     3841 ggtcggcgag atccgtgggg tggagggatc cgtcgccttc tccgcgatgc agaccggcca
     3901 cccggtgatg tgcaccttcc acgccgcgtc cgtcgagaaa ctgatccagc gtctctgcgg
     3961 tgacccgatc aacatcccca agacctacgt cgacaactta aatctggtca tcatccagag
     4021 cgccgtgaaa ctcccccatg gcgggaccgc gcggcggatg ctcagtatca acgagctcgt
     4081 gggctacgac cccgagaccc aggggttctc tttcatggcg gcgttcgtct gggaccctgc
     4141 gaccgactcc ttcaccttca caggcagggg gagcagtttc ctgctcgaga acaagatcgc
     4201 aacgatgctc ggcatccccg agaaccggcg ggccgatatc tacgatgaag tcgataaacg
     4261 tgccaaaatt ctcgagcggc tccataaggc cgggtacacc cagttctggg acctcttcca
     4321 catgatcacg aagatcaaga agcaggggct gctcaccatc gaggtgtgat gtgttcgagg
     4381 atgtagtcga ccggttgcgg ggggcaaacc agaggaagat accgttcgag gagcagattg
     4441 catacctcgg cggcctccgg tcctccatcc tcgagaacaa gaagatggag caggacctgc
     4501 tcttcatgta cacctacatg gccgcgatca ccacgtcggc cgtgacccgg cccgagatct
     4561 tccagtacac ctccgagcgg ttcgagtaca tcccgtcgcg ctacatcgcg aaggtccagc
     4621 gtctggtcgc cgggtggggc cagagttacg ccagcgggct ccgggcggtt gccgagcggt
     4681 gccggaacag aaccctccag agcatgctca accgctacgc aaactccatc gactccggtg
     4741 tcccggacga cgacttcatc ggcacggaac tctcgagtat ccggagcatc taccggaact
     4801 cgttcgagca ggggatcgaa ctcctgaaga agtggggcga cgcctatatc gcgatgctcc
     4861 tctcgggagc gctcgtcgcg atcatgatga tgatatcggt ggctatctac gctcccgacg
     4921 gtatagattc ggcactgaac tccgcgtaca tgatcatctt cgcgatatcg gtcttcggcc
     4981 tcgtgatcat gtaccgggcc gtccccgacg acccccgcac gcacgcactt cccggggtct
     5041 gttcgaagga gcagggcatc atccggagac tggaacgccg ggtcgtgctg atcgtcgccg
     5101 caatcacgct ggtgctcatc ctcgtcggcg ccaacgccgg tatcatcttc ctgctcgccg
     5161 gcctgctcct gatgccgctc ggggtcatcg gctacatcga cgatgccaac gtcgtcaacc
     5221 gggacgcaga cttcgctacg ttcatccggg gtctcgggtc gatcatgggc ggcaagggga
     5281 tcaccaccgc cgatgcgctc caggaggtgg accggaagtc gctggtctat ctggagccgt
     5341 tcatcaactc ggtctcgtcg aagctgaacc tcgggcttga tgaggccggg agctggaaga
     5401 agttcatcgg ggagaccggc agttacctga tctacaagta catgaacatc ttccgcgacg
     5461 cggttgccct cggcgggtct cccgaccaga taggaaagat cgtcggttcg tcgatgctcg
     5521 aacaggtgct gctccggagc aagcgcgaca tgatggtgaa ggggttcgtc gtcctgctca
     5581 tcccgatgca cggggcgatg atcgccatct tcgtcttcct cttcgagatc ctcctctcca
     5641 tgtcccgtgc ggtgacggaa gtgatggaac acttcgcaga gacctcggcc gcgctctccg
     5701 gggattcggc ctcgatcggg agtaccatgg gatcttcctt aaacatcttc gtaaacttcc
     5761 ccgaagacac gatgcgaacc tacgtggtga cgatcctctt aatgctgacc gttgcaaaca
     5821 tgcttgcagg aaagattgtc atgggcggcg accgatacct gtactacttc tttgcaagcc
     5881 tgctctgtgt ggtcaccggc gtcatctaca tcgtcgcacc gatggttgtg agcgtattct
     5941 ttaccattcc aacatttgtg ggggtgtaat gtgagagaaa acctgcgtcg tccgcttctg
     6001 ttcctgatca ccgtcggtac aggctccctg atcatccttg tcgatgccaa agtcgaggtc
     6061 atcgtcgcag gcaccgtgct cgcgggattt ctcgcgctcg ttgtcaccgg gaccctggac
     6121 cttgccgaac tgaaaccctc gaggctgcgc tccgcgttcc gggagcgcgg ggagaagaag
     6181 gaagagttta aggcaacgga cgcgaaaaaa ccggacccaa cggcagagaa accctcatcc
     6241 gccgggatta acctgcccgg catgctcggg accttcgcgg catcgattcg agagacgatc
     6301 gcccacgccc gtgcccctga aggtgagaag gagaacaaga tcgagaagct cgatgccatg
     6361 ctcgaccagg cggtcgacgg cgcagccccc gaaccgtcca tgaccccggt cccgccgaag
     6421 gccggcgggg gctcggtcga cccccttgcc tcacttgcgg accttgacct cgactcgctg
     6481 gagggcttcg acctcgacgg cgacgtatcc ggttccggga cgatctttga gtccgaccgg
     6541 atctccctcc tctccgggga agatacagac gccatatcgg agatcctgaa agcgcatcag
     6601 agcgacctcg acgacctcga actcacctcc ggcatcggtc tctccggcgg caccggcgaa
     6661 cccgttctgc cggtagcagc agacgtcccg gaactgcccg gaggcgacgg catgccggat
     6721 atgagcgccc ttagtgagga actctctgcg ctcgacggcc tggacgttgg tgagatcgag
     6781 atcgagggcg aggaggaaga ggttgacgag gaagagccca tcgccgacga agaggccctt
     6841 gatgggatag aagaggagac gaaagagaat ttcgatatcg tctcctttgc gtccggcggc
     6901 acggtcgacg acgacctgat caccgcactc aagtccgacg caaagaagaa gaaattcgtg
     6961 gaggatgtca gcctggtccg cgagttgcaa ggggagcgat accatgcaaa agaccttgtt
     7021 gtggaacttg aggatatcct cgaggcgatg aaagcacaac gatgatggta tcctgaggaa
     7081 gattagtaga gagggggtga cggagacaac atgaaatcgg ttccggtaaa ggtcgagcac
     7141 gaaggtaagt gggtcccaac gacgatgggc atcggtgagg accgtttccg catcgatact
     7201 cccctcaata gggaaattct ctataaatcc gtggtcgatc tcgatgagaa gaagaaccag
     7261 gtggtggtca ccgccgggcc gaatggagag tcggtctacc atatcgcgtc ggttgagaag
     7321 gttctctcgg tcttaaagaa gttcatcatc acgcaggcga gcggctaccg gctgaacgca
     7381 ttcttcatgt cacccgcagt ccgcgggggg gtgctcatcc aggacgccca gtgggagaaa
     7441 ggagcgatcg ccgtcatgaa gaccggcatc tggttcgtca gccaggagag tcaggtctgc
     7501 atccccctcg gggacatagc cggcatcgaa ctcacatccc gggagatcca ggagaaggat
     7561 ctcaacgtcg tgaagatcga tcacctcggc gagaacgaag tcgtgacaag tttcgtcctc
     7621 tgcccgatga cgacgcttca ggtcctctac accttcctca aagaggcaac atatggctcc
     7681 gaagtcagcg aggagattga ccccctgacc gggcaggtgg gcatgctggt ctacagcggg
     7741 atggattcgg gcaccatcga aaacatgctc aaactctcgc ataaagaact cgatgccatc
     7801 tatgagaagc tcctcagcat ggggcttgcc gaagtgctct acatcagaaa agaagtgcaa
     7861 ttaaccgcaa aaggcgtcag atacatcaca gaaacagtaa aatcaccaat ggattaatac
     7921 tattttttct tcagaataag aaggcatata tagcgtattt ttcaaataaa cactgagtgg
     7981 tgtttgatgg ggagaattct gatcgtcgat gatacaatgt ttatgagaac gctgctgaag
     8041 aacatcctct tctccggcgg gcatgacatc gtcggtgagg cggcggacgg ggccgaggct
     8101 gttgccagat accgtgaact caagcccgat ctcgtcacga tggacgtagt catgccgaag
     8161 gtgaacggga tcgaagcgct caaggccatc aaggccgccg acccttcggc aaaggtcatc
     8221 atgtgtacgg cagtcggtca ggagcagatg gtcaaactcg ccgtcaagag cggtgcgaga
     8281 ggctacatcg tcaaaccgtt ccaggcccca aaggttctcg aagagatcaa gaacgtcctc
     8341 agtgcataac catgatacgg gttctgatcg tcgacgactc actctttatc cggacaatcc
     8401 tccgggatct gctcaaagac agcccggata tagaagtcat cgggacagcg gtcaacggca
     8461 tcgacgccct cgggaagata tctgaactga agcccgacgt cgtcaccctc gacatcgaga
     8521 tgccccggat gggcggtctt gaggtgcttg aagcgctcag ggaggagaga gcgagaccga
     8581 aggtgatcgt cctgagcaca ttgacgtcga ggcaggccga tatgacccac caggcacttc
     8641 gactcggggc ggatgacttc atgctcaaac cgagggatgt tccgaacgtc aggggcgtcg
     8701 agagggaact catccagaag atcaggaacc ttgtctctct cccgacgatg gcaagacccg
     8761 ccgcggtccg ccagaaggag gcggatatag tggtgctgat tggctcttcg gccggaggac
     8821 cgccgatgct tgatgcgctt ctctccgcgc tcccgccgga cctgcctgca gcggtcgtcg
     8881 tcacccagca tatgcccgag gggttcaccg catcgctcgc cgagcgcctc agccgggtct
     8941 cgcccctgcc ggtgaaagag accgccaacg gagacctcct ccagaccgga gcagtcctgg
     9001 tctcaaaggc cgggttccac tccgtcatat cggggatcgc ttcgggtgcc gggagaaacc
     9061 atgggcggat catccactcg accgcccctc gcctgcatgc ggtccgacct gccgttgata
     9121 cgaccttcac ctccgcagcg cacgtcttcg gtccgcgcat cgtctccgtg ctcctctcgg
     9181 ggatgggaaa cgacggcggt gagggaaccc tcgcggtgaa gaccgcaggc ggcatcacga
     9241 tgatatgtgc tgaagaggac tgtctcgtat acggtatggc gcgatcggct ctctccaggg
     9301 attgcgtcga ccgggtggtt ccactcgcca atcttgcaca tgagatagcg cgagcagtca
     9361 gtcggcttga ggtgaatcat gtttgatgaa gagggatacc ggaagctgtt tgttgaggag
     9421 tcacgggaga accacgagaa catcgtgaac aatctccttg ctcttgagag cggggaagac
     9481 cagcagacag ccatcgatga gatcttccga tcggcacata cgctcaaggg gatgtctgca
     9541 tcgatgggct ttgatgcgat ggagcacctc tgtcactcga tggaagacgt cttctcgctc
     9601 atcaggagcg gccagcgtga ggtgaccgca tcgctcaccg accttctgct cacctgcacc
     9661 gacacgatcg aggggatgct ggatgctatc gaggcgggag gagctccgtc tgacgagcag
     9721 tccgggagtc ttgtgcagga actccgggca tttaccgaag aagagtcccc ggatgggccg
     9781 gaaatgtctt atgatgcagt ccccacgaaa acccccatcg agccggctgg agaggccgac
     9841 ggccgtccgg tctacacgtt aacggtcgcc gtcgatccgt ccagctccat gaaggacatc
     9901 cgggccatgc tgctcctgca gaacctccag gagatcggga cgatcctctc gacaaccccc
     9961 tcgcaggaac tcctcgagga cgggaagttc gacgggccgt tcgagatcct catcgagagc
    10021 gaggcagggg aggatgcgct caggaccgtt gcttccggca ccgagatctc ccttcttgct
    10081 ctctccttct cgcaggcgcc gcggacagtt cccccgaagg tggacgagga gggccagaca
    10141 cccgaaccgc agggcgcggt gccgtcgcca caggttcaga gggcggcgaa gaacgagaag
    10201 agccgggaga tcaagaatat ccgcgtcgat atagagcggc tcgaccggat gatgaatctg
    10261 gtcgaggacc tcgtgatcaa ccgcggacgg ctggaactga tcgcgaagaa gcacgggatc
    10321 aaggagttcg aagaggcgct ctccatggtc ggccgctcgg tctccgacct tcagaacctc
    10381 atgatgggga tccggatgat gccgctcgag cgcatcttca accgccttcc ccgggtagtg
    10441 cgggacgtcg cgaaccacga cggaaaggag gtagagttct tcatcgaggg cggcgagacc
    10501 gagcttgaca ggagcatgat ggacgggctc tccgacccgc ttctgcatat catcaggaac
    10561 gcggtgaacc acggcatcga gaccccggag gtccgccagg ccagcggaaa accggcgaaa
    10621 gggtcgctcc ggctgacggc aaggagggac aaggataacg tcgtcatcga tatcgaggac
    10681 gacggtgccg gcatcgatcc cgagaagctc cggaagaaag gtgtcgagaa aggcctcatg
    10741 acgcctgaga cggcggcgat cgcaacgaaa gacgagctta tcggcctgct cttcgagccc
    10801 gggttcagca cggccgatgc gatcaccgac atcagcggca ggggtgtcgg gctcgatgtg
    10861 gtcaaaaaga cgatcgcgtc gctcaaagga acgatcaggg tcgaaagcaa ggtaggcacg
    10921 ggaacgaagt tcgagctggt gctgcccccg acgatggcga tcatcgacgt catgatggtt
    10981 catatcaacg ggatgcggtg tgccatcccg atcagcaacg tcgtcgaggt ggcgcagacg
    11041 agactggagg cgatccaccg gatcggcaac agtgaagtga tcctgctccg ggaccagact
    11101 ctccccatgc accgcctgga ggatatgttc ggccgggcac agaagggcga cacgctggtc
    11161 gtgctgcaaa ataatggcag gagatgctgt attgctgtcg ataccgtgga cggccagcag
    11221 gaggtggtgg tgaaaccgct ctccaggatt gcaggcagat gtcgcgggat cagcgggatc
    11281 accatccccg gcgacggcga tgtcgtgccg gtactcgatg taaatacaat gatttagaga
    11341 ggttttctat ggaactagat ccgattcaga aagatgcgct ctcagagttt ggcaatattg
    11401 gtgcagcaca tgcggctacg accctatcgc agatgctgat gagtccgatc gagatgacgg
    11461 taccggaggt gcaggccatc gatatcgccg atctgcacaa ctatatcggc gacgagatat
    11521 cggccgtcgt cgtcttccag attcagggag aggttgcgga tggagggtat atcgtcgtca
    11581 gcatgccccg ggagaccgtc atccagctgg cgaaccagat gctcggcacc accgataccg
    11641 accgtgagct caacgagatg gaccagagcg ccgcaatcga gataggcaac atcatgatat
    11701 cggcgttcct cgatgcgacc gccgaactgc tgggtatcgt catgctgccg tctcccccgg
    11761 ccctggccat cgatatggcg catgccgcct tcgaatccat cgtcgcgcag gtggccgggg
    11821 acgtgaacga cgtcctgatc ttcaacaccg agctcacgag cgaggcgccg cccgtctttg
    11881 ggagcatcta catgctcccg aacgccgaac tcacgcacca gctgttcagc atgctgaagc
    11941 gtctgatgga accgctctcg taaccggcct ccgcgatacc atggagaaca atttttttgg
    12001 tgaagagacg gccgtaatcc tgggtctggg tgaacaccgg gtcgggacgc acccgatggg
    12061 gacaatcggg cttggttcct gcatcgcgct catcctccac gaccagcgga agtctcttgc
    12121 ggggtttgcc catatcatgc ttcctgaaag ccgcgggaat ctcgatcgtc ccggtaagtt
    12181 tgcggatacc gccacaaccg tcctcctcga agagatggag aggctcggaa gcacgaagtt
    12241 ctcgatcgcc gcactcgtca tcggcggtgc gagtatgttt gagttctcag ccagctccct
    12301 gaatatcggc gagcgcaacg tcgccgcagt caaggacctg ctccgcgaac agggcatcag
    12361 gatcgagcat gaggagaccg gcggaaagat cgggcggtcc gtgtattact ggccggaggg
    12421 gaagggccgc ctcatcatca agaggtcgaa cgggacatgc atcaccctct gatcgcctta
    12481 atcgctctgc tctctcttct cattctccca tcgtccgctc tttacatcga agcacccgat
    12541 tatgattcgg gcatcgcatc cgccggagtg aaggaggcga tcaacggccg gtacgg
//
LOCUS       DAJB01000093            6766 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_93978, whole genome shotgun sequence.
ACCESSION   DAJB01000093 DAJB01000000
VERSION     DAJB01000093.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_93978"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1458 a         1959 c         1855 g         1494 t
ORIGIN      
        1 aattaggaga caaagagcac tagtgaaaag tggaaagtgg ggatatcctc ctttctatag
       61 tcaaatctca ttcttgggtc atatcggcat tactattgta ttgccaactc gatcatcttg
      121 atctcttctt cagagagtcc gtataactca tacaccagcg cgtcaatcct tttatcagtc
      181 gcctcgatct cgcgcctgat aaaagtttta tcgtgctccg tcttcgcgct gctgagatgc
      241 tggttcagat cgaacgtctc ctcgataaga gcgttcatac gtgcatagtg ggcgacgtcg
      301 gcatggtcga tggggcagat tgggagtttc aatagatatg atttattcat atcatcagct
      361 aggaagtttg tacaatagta attcatcagt ttcgaattca atatgcctaa taagaagatc
      421 aaatcagatt ttctgtcttt attggatacg attgtttgaa gggttttcat acccgctgaa
      481 ttctgtctct cgtcaaaggc gcaaaccaat cgctgtttcc atctcatttt ttgaattcgg
      541 gtaatccaaa tttttggatg ggtataatat ttttcaacct ttgttggaat gagggtattt
      601 ttcgatattt gaatgccctc cctgacatta tacctctcga tgtcgttggg gataaccatc
      661 caaaaatctg agccttcaca ctcttgtaaa gaaaatttac tcccttcttc ccctcgcgag
      721 acactgaata actcggataa cgcatctcgt acatcacaat tctcaatctt actgatgatt
      781 tgctgatctt ctatagatga taaatatcca atgatttggt taggtcggtt tttccatagg
      841 tactgaggta cctgtgatgt atgatcccac tcctctctgc caaatttgtt cagtctttct
      901 tccgcagata ctacaaacct gctaatattt ctatgagtga taacattact atctgttggt
      961 ttctcattca tcaatctgaa cacgagagcc caagtatctc ttacgttagg aaataccggt
     1021 ccagtttcga tcatttcaac tatcgaataa tggtcaagga taagattacg aagggactga
     1081 tgatcgtcct ttcgcaaaag ggtatctgga gtgatataac taagttgacc ttttttagtt
     1141 agcatcctga gcccaaattc aataaatact gcgtaagaat taatgtttct accactggcc
     1201 gcagaatagt tctttttcag atattcttgt tcggacaagc taaacgatga accccaagga
     1261 gggtttccaa tcaccacatc gaacccgcca cgctgcagga tcccagcgaa ttctcgcgcc
     1321 cagtcaaacg cattgagctt cttgcgtccc tcgatgctag gcgggatgga gaaatcgtcg
     1381 tagatgtcgg aggcgacgag cgagttccca cacttgatat tttcgtgaag acttggaagc
     1441 gcacgttcag caaatagggt catctgtttc gatacaccct cttgactctc gtcttcgagg
     1501 actttgagga ggagggagag tttcgtcacc tcgacggcct gtgggtcgat atccacacca
     1561 tagacattgt taaggaggat gcgttttcgt tcggccgtgg tcagtctcca gtcgcttcgt
     1621 gtacgtgttt ctggtccatt tccagcctta tagatcggga gttgttgcgt tgcgccctga
     1681 cgcctctctc gtccctgccg gctcgatagg tcccgagcag gggggagcaa cgctcgaact
     1741 tcagatgagg tcgccccttt ctccttgatc gttgggacca agtgctcaat gtaccaatct
     1801 cggtgccagt tcaacaggaa ctgatatgca ccaagcagga aggacccaga gccgcaggcg
     1861 ggatctaaga tcgtgatgtc tgatacctcc tttggggtct tcccgcggac gatctcaccc
     1921 acgctctgac gcacaatata atcgacgata taggtggggg tgtaatagac accccctgct
     1981 ttgcggatct ctagcttctc ttccaccttc gcatggtggc cttccgtcag gcgaattatc
     2041 ttgcccagaa actgctcgta gacatggccg aggatgtcgg ccgggatggc ggaaaactca
     2101 tacgggcttt cggggaagta aagacggcgg atgatcgctt tcagcacatc gtcatcaatc
     2161 atgagtgtcg gcgttagggt atcggcctcc tcgctccgtc ctcgctcttt ttggaaaaag
     2221 aacaggcctg aattatactt atcgtcagcg tgacgaaaaa gaccgcagag gcgagaatat
     2281 gtttgccctc cttcgagcaa ccgttgcaga gtaccatatg gctcgatccc cctatcttcg
     2341 ctgattctga ggaaaataat ccggtcgatc gtccgctgaa tggccatgtt cagttcatcg
     2401 acggtgattg agggattgcg gagcgcgatg ttccgggcga gattctcgcg ccagacctcg
     2461 atatcagcga gaaacgcctc gtcgacgccc acggtccctt tcttatcggt ctctgaatca
     2521 gcatatttat cgaacgcgcc cttcagtatg catttttggg agaaaatttt tgcgatccag
     2581 ttccacttct caggatattc actataggtg aagtagcata ctcgggccgt ccctgcagta
     2641 tcccccctac ggagcggttt cgtgcagtca tagacggcga actcctcgaa gtttgtcagg
     2701 atgctcaact tcaacttgga attccacgca tacttgcgca gttggagagc tgactctgta
     2761 tcgtctcgga tcttaacgga tggcttcttc gcctcaacta tgaatttcct gacaccaccg
     2821 atccggaaag agtagtcaag gtaatttgtc tgcccgcgta tccgtatcgg gtcttcatga
     2881 gcgacctcct tgtaagcctc agagagtcct gcttggttat ccacatccca actgagcgcc
     2941 ctgaagaacg ggtcgagaaa ctctcggcgt agttgtgttt cgttgtattg tccccgtaga
     3001 tatgcggggc ggtgatactc aaaacgttcg acaagcgatc tgatggcatc agggcatgtt
     3061 atctgttcgg gcattcaatc acagccttct gatcaatttt tcggagcgga gattctaaat
     3121 aagttgcttt ttgttgaaat gcaaaatttt caggtggctg tgattgaata cgaaagagga
     3181 gacgcattaa tctgaagatg aaaagcatgc agacgttgca gaaatttcga aaacaggtga
     3241 tatcatagcc agtgtagacg ttcttcgaga cgcctctaac tctaaagagt ttatgtagaa
     3301 caagcccctc acctttgcgt tagaggccaa ccttactcat ccactccctg cgcgcatggc
     3361 tcttcaccta actgcttcag aacaggaacc acacccgatc tgatcctggg ggcaccgccc
     3421 ccccccacct caagagggag cccccaccct cagtgcatga tcgtacctca ggtggtgaat
     3481 gtcatcgcgc atcaaagagc aggcaccacc ctacagcagg atcgtacctc agagagtgaa
     3541 tgtcaaattt tttcgtgtta cctataaccc aaaacagcaa tgcgaggggt gggattcgaa
     3601 cccacggact ccttcgagaa cggatcttaa gtccgtcggt gttggccggg cttacctacc
     3661 ctcgctcaaa agatccaata actattgtca attgcacgta ataagtccaa cgctcgataa
     3721 gggcttcggc agacggcgag atctagtaac cttttttagt attaaatctt aagtccaccg
     3781 cctttggccg ggcttggcta ccctcgctcg aaaagatcca atacctattg tccattgcag
     3841 gtattaagtc cgacgcccgg ccgggtcttc ggcgaaaacc ggatccggca acccctctcc
     3901 gtcgcaaccc ccgggcccaa aggtatttga ggcccggccc ggaaagctgg aaactgccat
     3961 ggagacgaac cgtaacccgg acaggccaaa agtgccctac tggcacgtct ggaccgacga
     4021 ggacggcgtg agccgccagg accgctgccg gatgagcaac tttgagttcg cgagcatatc
     4081 gagcggtgcg gcgccgtcgt ggatcgacag actcgccgat ccggctgcca gcgtggtcgt
     4141 cctggtcctg cccgcaggct gggtgggcga gtggcacgag aaccctgagc cgcaatggat
     4201 cgtacccata tcgggccgct ggttcgtgga gaccatggac ggcacgcgtg tggagatggg
     4261 gccgggcgag ctctcgttcg gcaacgacca gaatacccgg cccgacgatc ggggacagag
     4321 gggccaccgc tcggggacgg tgggcgaccg gcccgcggtg ctgatgctcg tccagatgga
     4381 gcgggtcccg gcccccggcc ggccctgcct gcccgaagtg gaataggcta tggccccggt
     4441 tcaggccgcc agcctccgca ccacccgggc cagccggtgc atcccctccg cgatctcctc
     4501 ctcgcccgcc gccgagaaat tcagccggat cgtatcctcc ccgccgccgc ccacgtagaa
     4561 cggcgccccg gggaggaccg cgacgccctc ccggacgccc tcggaaaaga cgtccatcga
     4621 cgagaccccc tccggcaggg ccgcggtcag gaacatcccg ccctcagggt tggtgtgggt
     4681 cgtgcccggc ggcatcaggt cgtcgaggag gtcgcagatc tgccggcagt gcctcccgta
     4741 gaccgcagag acccgggcaa cgtgagcgtc cagatcgtgg gtcgcaaggt agcggtgcag
     4801 gatcacctgg cagaggaagt tcgagtggag atcggccgcc tgctttgcga cgttgaactc
     4861 ccggaggacc ggggcgggcg cgtagatcca gccgatccgc attcccgggg cgacgatctt
     4921 cgaaaacgac cccgagatga ccgtctggtc cggcaggtac cgcttcaccg gcagccgcgg
     4981 cttcccgtcg aagaaaagtt ctccaaaagc gtcgtcctca tagaagaccg tctccgtccc
     5041 ctcgaggacc tccgcgacgc cccgccgctt ctcctgcgaa tacgtccttc ccgaaggatt
     5101 ctgcgagttc gggatgccgt agaagaactt cggcgcgtgg tcccggacga gcgacgcaaa
     5161 cgcggtcaga tccggcccat cctcctcgag cgtgaccgaa aagaagtcag gctcgtagag
     5221 ggagaaggcc tcgatcgcgc cgagatagcc gggccgctcc atcccgacgg cgtcccccgg
     5281 gtcgaggaag atcttcgcga cgaggtccag gcactgctgc gagccgttca cgatctggat
     5341 ctcttccggg gtcgccgggg aggccgaggc gtctccggta acggtcggcg atgaactccc
     5401 ggagcggcag gtagccgtcc gtcgtcgtgt actgcagcgc ttcgcgtccc ccttccgcga
     5461 agacctcgcg ggccgcgtcc cggatccccg taacatcgat gtaggccgag cccggcagac
     5521 cgccggcaaa cgagatcacc ccggggaccg ccgagacccg gaagagctcc ttgaggaacg
     5581 actccgggac cctccccatc cgggaagaaa accggtactc catgatgagt gttgggcgtg
     5641 ccgaaagaaa aagtgccggg tagttcactg cagcacgatg taggccatga ccgagagcag
     5701 gaacgcgagc accaggatca tgaaactgat cggggcgatg aacgcgagca tcgcgttgac
     5761 ggtgcttgcg agctgcgaga cccgctgcga cccgaagacg acgatcccct cgcacgaggc
     5821 cgttgccgct cctacccggg cccccgagag gtcggcgact gcttcccgga ccgcctgctc
     5881 gacatacggg atgatctccc cgaccggcac cacgtagccg accgggttct tcccgctgat
     5941 ggtgttcacc gtgtgcgtat cggtggtcat gatctcggcc tcgtcgacgt agtcgagcac
     6001 cacctcgcgc agacgctccc ggacaccctg ggccatgttg ttcccgtcaa tcaggacgta
     6061 ggccgcccgc ttcccgccga cctccgtcgc cagcacctgc accccgagcg acccgaaccc
     6121 ctgctcgcgg gtgaacggaa cccggacgtg cgagaccccg atcgcgagcg gctccacggg
     6181 gagatcccgc gcgacggcga acccctcgcg tgcggcggcg atatactccg tcgcaatccg
     6241 cgtcgccggg aggaccgggg agcccacgct ggtcatgcag ttgtgggcgt ccacgaaggc
     6301 gacttccgag aaggcgcacc gtccttcggc catgatcgcc agaccgatcg agtaatcgag
     6361 gtcctccgtc cgttccgggg accgggtgct caccatcagg agggcatccc cgaaccgctg
     6421 gcagaggatc gcgaccgaac cggaggccac ccggaccggg cggctcgcgg tgccggagaa
     6481 ggtcagcccc tgccgggacg cctccacggc gcggccgatc tttgtaatct cgctctccga
     6541 gaccagattg aagtcgtggg tggcgcaccc gtgggcgacc agcgtctcct cggggaacgt
     6601 gtcgtggagg atccagggga ggttcccgcc gccgacgtcg cccatcggtc cggggtggac
     6661 gttcgggacc gtgaagagga cgtcctcccc cgggtcgcgg gagaagaaga gcgagacctg
     6721 cgggacgtag acctcctcgc cgatctcgcg gaagaagtcc tccatc
//
LOCUS       DAJB01000094           11030 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_16259, whole genome shotgun sequence.
ACCESSION   DAJB01000094 DAJB01000000
VERSION     DAJB01000094.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_16259"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2121 a         3481 c         3510 g         1918 t
ORIGIN      
        1 ggccctcccc gcgtggcgat aatcccacgg tccagtatac gggtgagccg gggaaacccg
       61 cactgctctc ctgcagtaat acccccggga cgaaacggaa tacgaacacc agaggtgcga
      121 actgcagaac tcaataggat ccaggcccgt ggtgagataa ccagtgctcc aggagaactt
      181 agtcatcgga accgggcacg agaaaaaaaa ggatgtagtc cccatccggg gacccttcaa
      241 tacttatacc accggccctt ctcgtcgtac tcgccctgct ccacacgcgg cgccgcggca
      301 cctgcgtggc tgtggaagaa actcgccttg taggcgtaca ggaacgctga gaagagcacc
      361 atcacgaccg cgtagatgac ggcggtgatc catattccct cggtcccgat gagggcaagg
      421 atatcctgcg gcatcatcgt ctggagctct tcggggccca tgcgggtgag cggttcgagt
      481 ttctccgcga ggagggcggt ccaggcaagg agggccgcga acccgagacc tgcgaaaaca
      541 acgatgtttg tcaggtagaa gaggaggacg ctgccgaggt tgttcatgac gaactcgatg
      601 ctccgccgga tcgactcaaa gacgttcgtc tcctcaaaga ccgctacggc atcgtagaag
      661 aacgtgaaga aggcgatcga gacgatcacc ccaaggagga gcggcgccat acctgcggcg
      721 gcaccggccc cgaggagcgc aagcgggatc gcaagcagaa agaccatcag gacaactcca
      781 aagaagatga ccagcgtcgg caggagtatc cggaagtagt aggtcttgcc ggactgcacg
      841 aactcactga aggagaagtt ccccgcacgg atcgtgccgt agacgccgcc ggccaggaag
      901 ggtagcgcga gtcccagcag gagcatcaga ggctccatgt aaaacgtctc gcctgagatc
      961 gtgatgatgg cggttgcaaa gaagaaaccg cccatggcaa gccctatcga ccatatgaca
     1021 gggtgccgga ggagtgaggc ggcattggtg acggatttga gcgtcatggt taccggactc
     1081 tcggcatggc gacgatctcg cgcacctgca ggtcaaagaa cgccgcggta tgcgcggggc
     1141 gaatgacgca gacggtatcg gcaccgcttg cggtgcctcc caccgcgatc acctcttcat
     1201 cgacggcgac cacaccctgg tctgctgcca tgaggacgca ttccaccgcg accttcaggc
     1261 cgaccgcgac ggtgcgccgc aacgcctcgg cgaccgcctc ggtgcgggag ctcccgccga
     1321 gtttcgacga gcgggagatc gcgcgctcga gcccggagag cgcgtgcgtg ccggtgacga
     1381 tcttcgcacc ctccccccgg agagtctcgg ctacgtcctc tgcgaactcc cactcgccgg
     1441 gtctcgagaa accgaccgcg tgggtgacga cgatcaggtc caggcccttc cccgacatgg
     1501 cgtcgcggaa ggcaagcgcc gtccggccgc tggtgctcgc gaccacaatc ttctggacgc
     1561 cgagttcccg cgcccgctcg acggcgaacc gggcggcatc acgcgtattc tgtgcgcccg
     1621 gagcatcgaa atagtagata tttcgggtta cgaagcccat gcagtcatgt tgttttcgct
     1681 cctaattgaa tcccccgttt tggcggcagg agagatttca ggggagatgg ggcggtgcag
     1741 aggaccgtga ccgcaagccc tgacccggcg gggtcaccgg actatcggcg gcaacagttc
     1801 cagcagcaca gcccgggcaa aagaaggtga taaatggtgt atgacccaac actactctca
     1861 accgaacggt gcaataggag ttactcatga ttacactggc cattgcagga aaacccaact
     1921 gtgggaaatc gacatttttc agggcagcga ccctggccca ggcagagatc gctaattacc
     1981 cgttcactac catcgatgcc aaccacggcg tcgcgtacgt ccggaccgcc tgcccctgtc
     2041 aggagatgca cgtcccgtgc gagaactgcc aggatggggt caggtttgtc ccgatcgggc
     2101 tgatcgatgt cgcgggcctc gtccccgagg cgcataccgg gagagggctt gggaaccagt
     2161 tcctcgacaa cctccggcag gcggacgcga tccttcaggt cgtcgatgcc agcgggtcga
     2221 cggacgccga aggaaacccg gtcgatatcg gctcgcgcga cccggagaag gatatcgagt
     2281 tcctccagta cgagatgtcg atgtggatgt acggcatcct ctcgcggcac tgggggaaaa
     2341 tccaccggca ggcccagggg aaagaactgc ttcttttaga ggcaatcacc gaggtcttcg
     2401 gcggtctcgg cgtaacgttt gaaaacgccc gtgacgccaa cgaggcggcc gaaatcaacc
     2461 tgctgaccgc cggggaggag gatctggtcc ggttctgcaa ggaactgatg acgatcagca
     2521 agccgatgct gatcgtcggg aacaaggccg accaggctcc agaggagtgc cttgaccgac
     2581 ttagaaagct taacatcatt attgcgagcg cggcagggga acttgcgctc cggatggccg
     2641 cggagggcaa gttcatccgc tacctccccg gcgaccggag ttttgaagtc agcccggaag
     2701 cgaaactcag cgccgggcag cgcgccgggc tgcagaaggt ggcggacttc atgcagacgt
     2761 tcggcggcac cggtgtccag caggcgctgg atgccgcgat ctacaacctc ctcgaccgga
     2821 tcgtcgtcta cccggtggag gacgagcaca aactcaccga cggcaagggc cgggtgctcc
     2881 ctgatgcgta cctcatgaag cgcgggtcga ccccccgcga ccttgccttc caggtccata
     2941 ccgatatcgg cgaggggttc ctgtatgcca tcgatgcaaa gaccgggatg cgggtcaagg
     3001 acagcctcga gttgaagaac ggcgatatca tcaagatcgt cagcgtccgg aagtgaggcg
     3061 ccggcacggt gaggccctcg gcctctcccc cgcgcccggg ggagtgaacc ggcacctccg
     3121 gcgagcgaat ataaaccggc aggcatttat atcgcattga gaaatattcg attattatga
     3181 gcggcgaggt ctatcaggcg caggtcctcc ggaacttctt cgacaccatc accggaacgg
     3241 acaggaacct gacccggatc tacatgtgcg tgatgagcct cgccaaactc cgcatggagt
     3301 ctcccgagag gatgacgttc ctggtggacc agctccgcaa gtccaaacag agacgggaac
     3361 tctcggtcga tatcatcgat tacatgtgcg acgtggcgcg cgacctcgaa ctcggcacgg
     3421 tccagaccgc gttcggcgtg aaagacatca acaccgtcgc cgacgagttc agcgggatca
     3481 gcctcgactc gctctgattt ttgcgccggg aaagggctcc gggctgcttg atggtttttg
     3541 ggagttgcat cgcggccaag caaggcagga ccccggggcc ctcacaggtc ccgcccttat
     3601 atcgcggtcg tcttccaggc cccgcgccgg tacatcgaga agaggatgcc tgtctgcagg
     3661 atagtcccgg agaccaccgc aagccagacc ccgaggatcc cgatcccgag caggtcgggg
     3721 agaatgcacg cgagcggcac ctggaagagg gccatggagg cgagcgtggc gtacatgggc
     3781 ttctttgtat ctcctgcccc gttgagcgcg aacgcgagga tgattgcgac ggcaaggaag
     3841 atgtagaacg gcgcgaccgt ctgcatgtac gacatgccga tctccgtgct cgcgccgctc
     3901 gggtcgaaga cctcgatgat cgcgggcgca gcgaagtaga acactccccc cacgagtgcc
     3961 atgaacccgc cgtttatgag cgcggagagt cggacaccct cttccgcccg ctcgggcttt
     4021 ttggcgccga ggttctggcc gacgatcacg gcggttgcgg tcgcgatccc gaatcccggc
     4081 atgagcgcga ccagttcaag ccttcccacg atgccgtagg cagagagggc tgcggtgccg
     4141 aagactgcca cgatcgccat cgtcgcgagg aacgtgacgc tccggagccc ggattggaca
     4201 gagttcggga cggcgacacg gatcagcctc caggccagcg ggatctcgaa cttcgtccgg
     4261 agcgagaagg ggatcactgt ccgtcccgag agaagcaggc caaacgctat ggcgaatgcc
     4321 acgctccggg atatgacggt ggcatatgcc gccccggcga cgccgcaggc agggactgcc
     4381 ccgtagccga agatcaggag cgggtcgagg acaatgttga ggaagttcgc gaggatcaca
     4441 aggagcatcg gcgtggtggc atcgccgcag ctctgaaacg ttgagttgat cacccagagt
     4501 tcgaccagcg tcacgctgcc ggtgaagaga accgtcaggt aggatgcccc gagggccgcg
     4561 acggatggct cggcgccgct accgctatga ggccccggaa gaggttgcct tcggtcagat
     4621 tggtcataga gtcaatttat ctttatagga ttccaggatc tggatgacaa agcgctggtg
     4681 ctcttccagt gcctgctcat cgtcgggaga ggtctcgatc ccgacggcgt agagcaggac
     4741 cagcgcgttg tcgaggtcga gcgccgcagg gtcttcgatc tggtccgggc gcagacggat
     4801 agcgagatca tcgtagtccg cctccacgaa ggtatgctcc gggtttgccg tcacccggga
     4861 gtcggcgagg tcgccggtac gctcccggag gatcagcagg gcacgctcaa ccgcgccggg
     4921 acgcggggtg atcgcatcga gcctggcaag tgcggccgtc atcttctcgg ccgcgctccc
     4981 atagagctcg aatttatacg tcagcaggga ctgagcaatc aggtgcccgg tcttctcgtc
     5041 gatcgtggcg aagatcggtt cgatccgagc gatatactca tttaatacat ctttcatggt
     5101 tctctcctgt ggtttggccg cattaaacat tttttccacc cgaaccgcga ggagggtgat
     5161 cgtcgccgcg aagagcgtct gcacaatcac gcccgcgtcg agatcgttgg gtatgacgaa
     5221 gattagacac gcgaagagcg gcgtgaaaag cgagacgagg tcgcgccgcg gtttgaggta
     5281 ggcctggtag cagaggatgc acgaggcggc cacgcaccct gcggtgccgg cccagacggt
     5341 gtaggcgtag gggaagccga cggaggcgag cagcagctcg atcccgattc ccccgaacga
     5401 ggccgcagga actgctatcc agtggcgtaa tgtagacccg aatggaatgg tgtttgtcgt
     5461 catcacctgt tctcaggaac agtttgtgcg cagagcataa acagggtttc cctttgcacc
     5521 tacggtgctc atgctccttc ccctgtggaa gtcgcacttc gcactcttcg agtgcccgtg
     5581 ctccggatgt tccgtccgtc gcacttcgca cctggcggtg cttctgctcc cggccggtca
     5641 ggcaggcggg aggtcgggca cgtcgacctc cacatccttg ccggcaagag cagccgcgcg
     5701 ggaccggagc ctctcgcttt ctgcagtata cgaggcctgc gacatctccc gctggttctc
     5761 cgcccggccc ttcgcggcag cctttgcctc gcgttcccgc tgcagttgtg cgagcgtagc
     5821 catgaggtgc tgctcctcga tcacgcccgg gagcgcggcc agggtctcct gcagtgccgc
     5881 gcgctgctcc cggacttccc tctgccgggt cagggtgcgc ctcataccgg caggcagggt
     5941 accggggtcc gagaagaggc ggacctcatc ctgattcttg agggcaagat ctccccgtcc
     6001 gatcatggcg agggtcgccg gcatcaccgc ttcggtcagg ctgaccaggc tctcctcgtc
     6061 atccgggagg ctgcccgtgt aggcatcgag ggcccggccg attgccgcgg cggccgtgct
     6121 atcccctgcc tttccggcca ccttctcggc cttccggaag agatggacgg caggggttcg
     6181 gagagctgcg atctcccgcc cggcctcctc gtccatggca tcgagttccc ggatcctctc
     6241 ctcgatctcc tgctttctgg cgtagtccgg gcccagtttg agggaagaga ggtcctgttc
     6301 ggccttccgg atcgctctct ccgcctccat aaccgccgcc ccaagatccc ggacctggcc
     6361 ggcggcggca tactccccac gggtccggga gagggattca tacgactccc ggacgtctgt
     6421 cacctgctgc cgggccgccc gggcgcgggt gatcgcctcg gtcatcgcgt tgacctctcg
     6481 ccccagatcc ctgacggcgg tgcgcaccac cttcatctcc tccgggtaga gggaggcgag
     6541 gtacttcccc tgtcctttca acgccttgat cgcgcccttc aggatctcgg cggcggcggt
     6601 gtagaaggtc tccgggtcgc cggaaggttc gcgggagagg atctgggaca tcgatttcgt
     6661 gaaccccggg agggcctttc gtgagatatc ccggagacgg gggtggacct cctcatcgcc
     6721 gtcggccgtc tccatacggg cgatcgcctc cctgaggctg ccgagggtgc cgctgatcgc
     6781 ctcccgcgaa ggtcccgttg cattggagag ttctttcccg atctcttcct cgcgggcatc
     6841 cagccatccc gggaggtcgt caaaggagag ttctaaattc tcttccacag gctcggttcg
     6901 ccgaaagagg tctcgcagtt gcttaaacat ggtccatccc tcaatcgctc cctgcaatcc
     6961 gctgcagccg ctccacgccg tggatctccc ggatgacgtc caccacggcg gtggggacca
     7021 ggtgctccca gggttcaccg tcgagcatcc gctgcctgat ggcagtgccg gaatgcgtca
     7081 gccgctcata catgtcgggc gactggacgt ccacccccgc ctcggtgaaa agctgcatga
     7141 cgagggggtt gctcgagtag accgtatcga acggcggcgt catcgcccgg acgtgggcga
     7201 cccagagggc attgcgctgg acgtcctcga tggggatgac gtagaacgga cactcgaggt
     7261 cggcaagcga ccgggtgagc atcagcaccc gttctcctgc agtgaagggg tttgccacag
     7321 tatgggagag ctgggcgctc cccaccccga tgacgatctc gtcggcagag caggctattc
     7381 tctccagcac cgactgatgc ccgttatggt agggctggaa ccgcccgaca tagaaccccc
     7441 tgctcatgcc cgcctgtccc cgtgtgcgat ctgcgcggca aacgcgcctg ccgtgaaccc
     7501 ggcgtcgata ttcacgaccg cgaggacgga gcatgcctgg agcatgctcg caagcgccgc
     7561 ctcgccggcc cccatgtagc cgtagccggt gctcaccggg acaccgatca ccgggcggtc
     7621 gacgagcccg gcgacgatcg ccgggagcgt cccttcccgg ccggcgatca cgacgaagac
     7681 gtctgccggt atcaggtccc tgagcgccga gaaaaggcgg tggatccccg ccacgccgac
     7741 gtcgtaagcg gtcctgacct cgcaccccat ctcctcggcg atcaacctcg cttcttcggc
     7801 gacagggatg tcggatgtcc ctgcagtgag gatggcaacg gtcccccccc gtgaggcggg
     7861 cgcatctccc gtcgagagga cgatggcccg tgccgccttc cggtgctcca tccggacctc
     7921 cggcggcagc cttgcctgca gggcaacgag gtgctccggt gagaccctcg tcgcgacaca
     7981 ccggcccgat gccttcacct gcgcgagcat gatgtttgca aaggccagag gatcctttcc
     8041 ctcggcgagc accacctcgg gcatcccgca gcgcacgccc cgtccagtgt cgatccgggc
     8101 catgccgtcg atcatctcga tgcggagccc ttcgatggcg gccgtcgcct cgtcctcaga
     8161 tatctcaccg ttacaataca tccggaggat gtccctgatg gtggcattcg gcagcatagt
     8221 tcgataacaa aaataggggt gtaagtataa tattacttca tctcatgtcc tatctagtct
     8281 acgtcgcagg gttcggcctt gccgtcaccg ctttcctgtg gctccgcgac ctgcggatct
     8341 tctccagaac aggccttccc ggctaccgga cggcggccta ccggggagta ccttatacgg
     8401 ctctcgccct gctcggcttt tttgtcaccg cgtttgccga agcgtgggag tacctcggcc
     8461 tcggcctggt ccttttagcc ctctaccttc agggccgggt cgaccgggag aacgtatggc
     8521 acggtgaagg gaccagcgcg cggttcttcg gcagggcagg gcgcacaaaa gataagggct
     8581 cacgcaagag actttagttc aggagagtac cgaatgcttc agaaaccacg gggaacgcgg
     8641 gattttttac ccgacgagat ggaacgacgg cggctgatcg agcagaggat gcgggaagcg
     8701 acccgcaagt ggggataccg ggaggtctgc acgcccgact tcgagcacct tgaactcttc
     8761 acgatgaaat ccggcgaggg aatcatccag gagatgtacg tcttcgagga caagggcggg
     8821 aggcagatga cgctccggcc cgaggtgacg gcagcggtgc tgcggatgta cgtcaacgag
     8881 ggcaaagtcc tccccaaacc catccgctgg tgctacttcg ccgactgttt ccgctacgag
     8941 cggccccaga agggccgtta ccggcagttc tggcagttcg gcgtcgagct gatcggcgcg
     9001 gacacggcaa gcgccgacgc cgaggtgatc atgctcgccg acgagacgct ccggtcgagc
     9061 ggagtcacgt tcgacctcca tgtcggccac ctcgcgccga tgaagcatct cctcgccgac
     9121 cttgcccccg gcgaccagcg ggcgatcatg gcctgtctcg acaagcacga ccaggaaggg
     9181 ctcaaagcgg cgctcgtcgg ccgggggctc acccgcctca ccgagcccct tgccgccctc
     9241 tcggagtgcc gcaccatccc cgagatcttc gagatcgtgg gcgacgtccc ggaacgcgcg
     9301 cggatcgaag agacgtttgc actcctcgac tcccagaata tcaactacca gccggacttc
     9361 gggatcgccc gggggcttga ctactacacg ggaatggtct ttgaggcgtt cgccaggaac
     9421 ctcggcgcgg agaaccagat cctcggcggc ggcacctacc ggctcgccca cctcttcggt
     9481 ggcgacgacg tcgcctcctc ggggttcgcc atagggtttg accgggtcat ggtctcggtc
     9541 ggggactttg cgctggagag cagacctgtc gtcggcgtcg tctgcatgcc cgaagcacgg
     9601 gcacgggcgc tcgaggttgc ccgggccttc cgggatgcgg ggatccgggc cgaagccgac
     9661 ctgatgcagc gggggatggg tgcccaggtc tctcacgccg ccaggaccgc ggactttgcc
     9721 gtggtcctcg ggaagcgtga ggtcgaggcg ggcacggtga ccctcaggga tctccactca
     9781 ggcgagcagc aggagaggag cctcgaggac gccatcgctg aggtagcacg gcatggtgct
     9841 tgctgaagac ctcgccaaac agcagcggag catcagcgtc gcggagttct tcgagaagaa
     9901 caagcacctg ctcggcttcg actccccgac ccgcggcatc atcaccacca tcaaggaggc
     9961 ggtggacaac gcgctcgacg cctgcgagga ggcggaggtg ctccccgaca tctttgtcgg
    10021 cgtaaagaga atcgatgccg aggtctaccg gataacggtc gaggacaacg gccccgggat
    10081 cgtgccggag aacgtccccc tggtcttcgg gaagctcctc tacgggtcgc ggttccacca
    10141 gatccggcag agccgggggc agcagggcat cgggatatcg gcggcggtgc tgtatgcgca
    10201 gctcaccacc ggcaccccgg cgctggtcac ctcacggacg ggggcaaagg cgagggccca
    10261 ccggttcgaa ctgatgatca aaaccgagac caacgagccg gaggtcgtca gccacgaaga
    10321 gatcgactgg gaccggatgc acggtacccg gatcgagatc cagttcaaga gttcccttgc
    10381 cgcaaagaag cggctggtgg actacctccg cctcaccgcg atcgtcaacc cgcacgcccg
    10441 gatcacggcc gatatcgacg gcgaggtgac gacgttcgag cgggtagcga gcgaggttcc
    10501 cccgtgccca aagcccatcc tcccccaccc gcacgggatc gagttcgggg ccttgaagag
    10561 gatcgcggcc gcgagcaccg ggacggtcga ggagttcctc gtcggcagtt tctcccgggt
    10621 cgggaagaag accgcggacg agatgatcgc tcttgcaaaa ctcaaacccg cccggaaagt
    10681 gaacagactc gaatcggacg aattaaagcg gctcctcgac gctatgcagg ccgtgaaggt
    10741 tccggccccg ccggcgcagc agtgcctctc ccccatcggg gaggacctga tctgcagggg
    10801 gcttgagaag gagatccagc tcgacttcat caaagcccgc acccgcccga gcatggtcca
    10861 cggcgggcac tcgttcatca tcgaggcggc gatcggttac ggcggcagga tcccccccga
    10921 agggaacgcc cagatcttcc ggttcgcaaa ccgcgtgccg ctcctctacc agcagggcgc
    10981 ctgtgcgatc acgaactgcg tctcgcaggt gaactggaag ggttacggcc
//
LOCUS       DAJB01000095            7690 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_98891, whole genome shotgun sequence.
ACCESSION   DAJB01000095 DAJB01000000
VERSION     DAJB01000095.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_98891"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1484..1511
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4225..4307
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1386 a         2450 c         2400 g         1341 t
ORIGIN      
        1 tgtgtcccga gagccgagaa gaacgccccg atgaccatgt tctgtttttc catcctctgc
       61 tgccggtcgc gggacgcgag cacctggtga aagacgagcg tcaccaggag tacctcgagc
      121 gggaggaacg cgatgtcgcc gagcgtgtag atggcgatgt ggtagaggtc gccgaatacc
      181 aggaagtgca gggcgtagag cacgaccgag aggatgacaa gcgctgcccc gaaggtgatc
      241 agccaccgtc tgtcgttcat cgtaaggaac tatggtgccc tgatctcgtg aaccccgcgc
      301 tcccgggtgg agtgagcctg ctaccctcag accccatagc gcctccacgc ctcttctaca
      361 tgcagcgccc gcatcccgag cctccgggga gcatcgacgt cgttctcgaa attgtccccg
      421 ataaagagga cgtcggaggc ctggagcccc atccgcccga gagccgccgt gtatatccgc
      481 gggtccggct tctgatagcc ggggtctgag gagaagatga cgaagccgag acggtcgtag
      541 aggccgagcg cccgcatctc ccgctccgag aagacccgct gcccgttcga gacgaccccc
      601 gtcttctgcg tggaaaagat gtcgagcagc cttcggctct tctcgatgac gccgaggcgc
      661 cggagcgatg cggcccggaa cgcccgggcg gcctctacac cgagtcgctc cgtatcgatc
      721 tcccatacgc ggtgttcggc gcagatgccg gcaaagacct cctcgacccg gacctccgga
      781 tagcgctctc cggtccggcc ggccgcttcc cgtacccggc tcacgtagca cgcccgcagg
      841 cgttcgggcg cgatcatgac cccctgatag aggagccacc gggagaggcc ccggtaggtc
      901 tcgatatcgc gttcgtcggt caggacatcg atgagtgtcc cgtagcagtc gaagagaacc
      961 cctgttaccg gtggatcgcg gcgagacacg cctccgcctc cgatagcagc cagtcacgtt
     1021 ccgccgcccg ccagggcagc cgggcgatcc tgaggtgccc gagcgccatg aagaagggga
     1081 gggcggcggt atggcgccgg aattcttcaa cgctgtggct gtagtgccag agcaggtggc
     1141 cgatgtagtc ttcggccctc gtgctgctcc cgtccgatgc cttgatctct gcggcgagga
     1201 tgccgaggtc gcggaccggg tgggtgtggt tccgggcccc ctcgaagtcg attgcacagg
     1261 tgctgccgtc gaagaggtag ttgctgagcg tggcgtcgcc atggaccatg cacccaacgt
     1321 cggggccaat ccgcgggctg tgccaccatg ccccgagcag gcggttgaac cgttctctcc
     1381 gtggggctgg aaggccgttc tggtcgagta tcgcgtggaa gtaggcgaag tcgcgctccc
     1441 gtttgcagga ggtccgcgtg ccgtcgtgga gccgccggag cagnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn ntgttcgccg caagtgcacg gggaacgcgg atccagctgg agacatggcg
     1561 tagccggcgg aactcgttca tcatcgccgc atccgcgtta tagcgggtct tcgcacccgt
     1621 cggctcaccg aagaacttgc cgacgacgct gactgcggtg ccggcgaact cgtaccggca
     1681 gacgacgtgc gatgcaggct ctacccggta gacccggatc ttaccgcgcc acccggccaa
     1741 tcggtcggcg aggacgcccg cgagccagtc cccgaacgca tcgccggcct ccaggcgtcc
     1801 taccttcctc tcgacgatca actgctgtac caccggtggg gccggtggtt ctgcagggca
     1861 atgaagatac ccatggcatc ggggagaccc acaccttttt taccgcccgg gcgtcttaac
     1921 gggatgcacc ggcgatcctg tgggtgacga gccatgatac cgttttgcga tttcctggag
     1981 agaatcaggc ctgccgtcaa ccggcggatc gaggaggtga cggggggtga ggatgcaatc
     2041 gaccccggcg ttctcccgct tctcatcggg ggaaagcgga tgcgggccgg acttcttctc
     2101 tgcgttcata cctgcctcgc ccggacgacg gcacttaccg accgggcact cgacctcgcc
     2161 tgtgccgtcg agctcgccca cgccgcaagc ctcatcctcg acgacatgct ggacggggat
     2221 actgtccggc ggggagctcc ctccctccac ctcacccggg gagatggacg ggcgatcctc
     2281 gacgccgtcg gcatcctcgc cctcccctac gcgctcgccg ccccttacgg cgccggatac
     2341 gtgacgatgc tcgcgtcggc ccagcagagc atggcccggg gcgtggcctg ggagatgctc
     2401 ggggggcctg ggctcccggc ggccgaactc tacgatgcga tcgtcgcacg aaagaccggg
     2461 cgtctcttct cgctcgcggc cgcctggggt gccatggcgg cgggagagga ggaaacggtc
     2521 gtcgccgcgt tcgcgaacta cggtctctcg gccgggaagg cgatgcagat agccgacgac
     2581 atcgtggacc tgcacgcgca cgcggcgggg gcccaatccc ggcccggatc cgaggcgctt
     2641 ctcctccggt gcgtctgcgg agagaacggc acccggcaga cgccgggatc catacttgag
     2701 cgggagatag atggcgcgac cgcctggatc acggatgggg gccggcagcg ggtgccaccg
     2761 gagatgtggg aatccttcag gcaggtggtc cgggatatcg tggggctcac gatcgcgggg
     2821 ggtatgccgg gtgacgaaac agggcccctg accgcctgcc ccaacggccc cccgcaacga
     2881 taaataccgc cccgccccaa actcttctga ggaatcatgc cggtagtaac cattcgtatg
     2941 gcagagggac ggacacttga gcagaaacgg atgctcgccg atgagatcac cgccgcgatc
     3001 acaaggacgt tcggcgtcga tccggcagtg gttaccctct tcttcgagga actcaagacc
     3061 gagaacattg cacgggcggg aaaactcctc tcggagtcgt agtcccgcac cgggcgaaca
     3121 tttttcttta gcccgacaga gcctcatacc tccgggagtg aacggaatca tggcacgcga
     3181 acttgacgag aaagactttg ctatcctgcg gaaactggcc cccgagtaca gcggtatcct
     3241 ctgccccgaa tcggggcacg agttccactc gatcctcccc ccggtctcaa accacattgc
     3301 gaaggacgag gaggattttg ctgagcgggt cggccgcctc tcggacgagg actggggtta
     3361 cctgaccgac cagatcgtct ccggccggga ggacctcggg tgcatgccag aagaggatat
     3421 cgagaccatc ctcgtccgca tccagggtgc ggtgtcggag gaggccgccg aacaggtccg
     3481 caggctctac cacctctccg ggtgcgggat actgtagcgg ccggagaccg ggcgccatgg
     3541 ccgcatccgg aaacggtata tgccccggcg acgaccagac ctttatggaa ctaaaagagc
     3601 aactttccgg cgtccccggg atgcttcggc cattcaaggc gtacctcaag gaggcagggc
     3661 tcggtgcggg cgaccagatc gtctactacg gctgccccgg cacctgcacc cctttcatcg
     3721 aactcctggg gttcgccgtc cgtgacctcc ccgtcgagca gatctatgtg ccctacgcgg
     3781 acgaagcggc agcaaaggcg atccgcccgg tcgggggtgt cggcatgcag gtctccggcg
     3841 acgcggtccg cgtcgacccg aaggtgatcg tcctgatggg cgggcttgcg atgccgggcg
     3901 tcccggtcac gaaagaaacg atgcaggcgg ccgtcggcgc ccacgcgggg gcgaaggtcg
     3961 tgggtatctg cttcatgcag atgtttcaga aggcagactg gctcgaggcc ttcgacttcg
     4021 acctgatcat cgacgcctcc atcgacccgg tcagggtctg gcgctgatca ccctgccggt
     4081 cgttggtgca ggagataccg gcggctcccg cggcaggtcg gaccggagga gggtgaaccg
     4141 gaacaccgcg ccgcattcgg gatgccccgg cacccggtcc tccacccaga tccttccgcc
     4201 gtagcgtgtg atgagcatcc tcgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc gggaggaggt
     4321 cgtcggccgt gacccgaacc tgctttccct cgtaggcgat atcggcatcg gggtggtgag
     4381 cgatctcggc cctgatgacg gcgtcgaggt cgacgggtct tgttgccgtc tcgtgggaat
     4441 gcagccgccg gatcgtcgag acgttctgga tgatctcgat gctccggctg ataccgctcc
     4501 tgagtttccg gatcatctcc ctctccttcc cctccagcgc ctcgaccagc aggtcggcgt
     4561 agccgagagc gacggcgttg gcgttgttga tgtcgtgcgc catgatgtcg aggtagaggt
     4621 ttgcctcgtt gtgggccacc cgcaacgctt cctccgcccg gatccggtcg gtgatatccg
     4681 atccggaacc gaccagtccg accgggcgac cgtcatcatc ggtaaggact gtgtcctgcc
     4741 atgcgaggat cagttcgtcc ccattccgcg tgacgaccgg gttctctcgc cggggcaggg
     4801 cgttgccgct cgcgatcgcg gcgtcgaaga tctgccgccg tgcctctctg gatgcttcgg
     4861 ggatgaccgt tgtaaaccag tccttcccga caagttcctc ctcccggtag ccgagcaact
     4921 cgcagccgtg gcggttgatg agccggatgg tatgatcggc gttgatgacg acgaagagga
     4981 cgcccgccac gtcgaggtag cgctgcgcga gatccctctg tttcttcaac tcccgctccc
     5041 gccctttgag ttgctcggag agggaggtga caacgaaggc gatgacgacg aacatcaggg
     5101 cgcgcccgta atcgttcagc gtcaggatat ccggcctgta caggagactg ctgacgacga
     5161 cgagcccgcc gaggaagatg gcgaccagga tgctccgctt ctcccaccag agcgcggtca
     5221 ggatgatcgg gagatagaag aagtgcgaaa agaccgttcc gagcatcagg atggcgtgga
     5281 agtagtaggt caggaagatg gatatgccaa ggagggccat gataatggcc acacgctggt
     5341 tttcggtaag ggtagtcacg gggcgcccac ctcaactacc tatatttata tatttaatta
     5401 tataaaaatg tccgcatgta tattatccgc cgattctggc ggaggttccg gggaattggg
     5461 gtgaaggggg taacgtcgcg ggagataggg cgccacgggc cggttgggca cggaaccggc
     5521 gacccctcgt gcaggggtga ggcggaggcc ttccccccgg ctctcccgat acaggagcat
     5581 tctgccggat gcggccgatt ttttccaaac aacctatgtt taataggtcg tagagcaaaa
     5641 ctacttcaat cgcctgcttc accctggcgg ctgcccgcag gcccgcatcg cgtgaagcgg
     5701 aaccgtgcag cgtgtcgtgt cgccacctgc ccggaagacc cccggcgcca cgagaacgca
     5761 tccgctggtg aatccgtgtc aacagacctt actgaagcac tcgaagcgat gggctatgca
     5821 gatgccgaaa tcccgaccct ggtggagtca ttccctcccg ccgtcatcga atacctgcgg
     5881 gacttccggc tgcaggaggc ccgtgacatc atcgagacgc tcctctacat ctacatcgcc
     5941 cagaacagct cgtcatcccg gggagaaggg gagggcgtgg tcgaacagag ttgttacgcc
     6001 tatacgtcgg ggccgctctt tgccctcagg caggtcgacc tcatcgagtc cacctcctgg
     6061 cacggcacga cggtaataaa ggcgacctcc gccggagaag caatctcccg gccgctgatg
     6121 gaggcacggc tccaggcgct ggatattggc ggactggcac gcgagatcca cgaggtcgtc
     6181 ccggccctcc tcgcggggac cgtcaaaggg tcttttgtca acaagacctt cccctccacg
     6241 cttccccgca ccgaagagac attcgtcagt ttcctcctca acaacagtcc gggcctcttt
     6301 atcgagtgcg agcgattcgc cgcccggctc aaggccgccg gttgcgccgt cctcgcctac
     6361 gcttacgacc ttgatgggtc cagagccgac ggcgttgtct acacctttcc gccggagttc
     6421 gcctacatcc tcaaggggat gctcgagagg atagaccccg aggtccggga gcactatgac
     6481 atgctccttg aatcgttcta ctcgacgttc accgttctcc gctacctcgc ctgcggtgag
     6541 gactacgacc gtatccgggc atcaccggcc caccgccgcg aactgacgcg ggtcctctcc
     6601 gtgctcaacg acgcgatcta cgttcttgag caggtgcctc aggacgatgg ggtcgacctc
     6661 gcccggttca tcgtcaagga ccgcaacctc tacgatatgc gcctccgcga cctcgggcgg
     6721 gcgctgatgg ccgacgttgc cgcgaagatc gtcatcgacc ggcctgctcc ggccccggca
     6781 gcggaggagc ccctccctcc ccgtgaagag gcccccatcg tcctcgagga gagcgcggcc
     6841 atagcgcccg cgcctcaggc cggggaaggg gtggaggagg aggagtggga cgagcccctc
     6901 ttcgccgatg atgaggaaga nnggaagaag aggaggaaga gccgatcgcg tccgtgccgc
     6961 cgcccgcggc ctcctcaaac tccgttcctc gaagcctgac ggcctcccaa actctggatt
     7021 cccgcccgtc gtcgctcgca cctggcggtg ctcaaactcc tgcccttcgg ccagtcgttc
     7081 cccgcgccgg cggactcgac atattcctcg gccacgcccc ggatgggcgg cgggtcaact
     7141 ggtcgccggg acagctcaac aacggccata tgatcatcct cggcggctcg ggtgccggca
     7201 agaccgagac aatccggtgc atcgcctcgg atctcgcagg acaggggctc cccgtcgtcc
     7261 tgatcgactt ccacggggac atggccgcga gcaatggcga tatccggtcc tacaagattc
     7321 gcgagggcgg gcagtactac ttcaacccgc tggaactcga ctcctccatc gatgagataa
     7381 ccccgctccg ggccacctcg gacttcgtcg atgccatctc catcaacttc ccgacactcg
     7441 gcatccagca gcgcagaaag atcaagaaca tcatcaagga ctgctaccgg atctcgggga
     7501 tcacgggaaa caccgatacc tggacacggg tgctcgactt cgacgacatc gagggcgaga
     7561 tcatgggctg cgaggacgag gcgatcccgg cctacctcga ggacatcttc gactacaaac
     7621 tcttctcggg ggaggagaag atctcacttc ccacgatcct ctcgggcggg atcacccaca
     7681 tcaacttaaa
//
LOCUS       DAJB01000096            2756 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_231875, whole genome shotgun sequence.
ACCESSION   DAJB01000096 DAJB01000000
VERSION     DAJB01000096.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_231875"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    453..484
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          444 a          899 c          809 g          564 t
ORIGIN      
        1 ttggtgctct ggcatatgtg atatcggatg cccaagaaga tgttaccatt aacaccacta
       61 cttccagtgg caaaattatc tttcaggaca gtacctcgcg tggtgttttc ttccttggcg
      121 aggcttcagc cgacttcggg gcaaacacaa cttnntcctt gatcgagacg gggatggaag
      181 taaacacctt cactgcctcg tggagaaatg accaagaaga taaaccaggc agcacaaaga
      241 aagccacctt caccctagcg atctgggatc ctgcagggat actccacagc accactcagg
      301 aatcggaggg gatccattcc ggaacactga gcgtatcctg ctccccgttt gaacccggtg
      361 agggacggtt cgagctcaca tcgaccatcc atgagaggcg gctgaacgtt tcggcggtgt
      421 tcgaccactg ataccaacga ttttcaggcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnngtttat aggagaacgt cgacttctca cctatcgata ccggaaccgg tatcccaacg
      541 cggtgaccgg ccagacggac gattgccgcc gtccgggagg agagaaccat ctctacagcg
      601 ataccagcag aaccaaccat caccggcccc gggaaactct tccggcaact caatgagaag
      661 ccgttgcgga acgatctcgt attctacgct gcgatagtcg cagccgcatc gatcctgttc
      721 atggtgttgc ccctccttac ggggccatat caggaagagg gactggaccg gatcggggcc
      781 gtggtgtcgg gcaccctgat caccctgata caggggctga tcaccggggc cgttgccctc
      841 ctcgcgatca gcctcgttga acactttttc ctcctcttcg tcgatgtcca ccgggagttt
      901 gagctgacga tgaaatcggc gatctacgcc ctgtcgccct gtattctctt ttcctgggcg
      961 gtcctcctga agatcccgtt tgcaggcctg ctgctcctct gctgtttctg cctgataacc
     1021 tatttcggcg tacgggtgtt ccacgaactg tcaaaggatc gggccgcctt catctctctt
     1081 gcgacaggcg tggtgctcgc gatctatttc cgtagatggg tcctcgatcc cgtgcaggtc
     1141 ctgttatcct cctggacctg accgggtgga tcgcctgtgt aagcccgggc ttccctcccg
     1201 cctgcaacgg tctgttccag ggatggaacc cggctgcaac gccccgacgt gtagacgatc
     1261 gtgacagaaa tcgtttatag aaggacgttg acttttgtcg cagagtaacc cgacagccga
     1321 accgcggagc ccatccccgg ttcacatccc ggtatggtgc agatatgtca ggcagagtca
     1381 tccccnnagc cggctccggc tgctgatccc gccgctcgtt gcggtcggtg cgtgtgcagc
     1441 ctgggcgttc ttcgtcgaca tcgccgggac cccgggatcg gtgttgcagt ccgtcctccc
     1501 cgtcttcgtc ctcacgatcg tcacccttgc ggtcgcggga gccgcgacac cgctcccgct
     1561 catcgcgggc gccgtcccgg tccggagaga gcggcaggcg atgctcgtcg ccgttgcagc
     1621 ctcggttccg ttcgtcttcg ccctcctgat aaacccggac gggtggccgg gcgggcccgc
     1681 cccgctcatc gcggacaggg tgccggtatt cgggtggctc ttcgacggca tcatgagcgc
     1741 cctcccgttc gccgcgggca cgcccgcgta taacctgctc ttctccgcct tcggggtttt
     1801 cgggttctac ctcgagtgcg tgctggtcgc aaccatactc tacgccgctc tccgggtgat
     1861 cgccgccccc ggcccggggt tggagggaga ggggcacgcc gaggaggagg gatgagaatg
     1921 gctccggaga gaacagcgct cgccctcgct ctcaccgcgg cgtacttcat cgccgttccc
     1981 gtgacaggga tgtatgcccg gttcgcctac aacggccagt ccgtgtaccg ggtcgcagac
     2041 ctcgcgccgg tgctcctcct cttctgcatc atctcacttg cgggagcggt gctttgtgcc
     2101 gcggcaatgc gggaggaaga cggcaggtgg tgggcggcgg tgcccctcgc ggcgctcctc
     2161 ctggcgctcg ccgtcttcgg gccatcgccc tggtcctccg ggagcggcct gctcgccacc
     2221 gtgctcctcg ctccggcggc cctcatcctc ctgctcggag cgctcccggt caggatggga
     2281 acgttcgcgt ggttctcggc gatcnnagac ggggatcatc agcctgttcg gcctggtctg
     2341 ggcatacggc ctcttcctcg ccccaccgct cccggaaaac gtcagggtcg actcgccctc
     2401 gctctacgag ttcgccgggg cggcttacgt ctacgccggg ctcgggctcc tcggcatcct
     2461 gctcctcgtc ctcgcctgcc ggcaaacgcc cgtcgaggag agggcggcgt gaagattcac
     2521 ggacgaaccc cacgaccgca tcanntccgg gtggaggacc ctcttccgct gcgcaatccg
     2581 gtagccggag acgacctgca gagagtcccc gacctcccgg tcgagcgttg cgtcccccga
     2641 gtcaacgtac aggaggggtg tcccggcgag ctttcccggc gtggcgacca cgatcaggtt
     2701 ccgtagtccc gccttccgga ggacgtccgg gctgatctgc tggttgcccc ggccga
//
LOCUS       DAJB01000097            2600 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_85446, whole genome shotgun sequence.
ACCESSION   DAJB01000097 DAJB01000000
VERSION     DAJB01000097.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_85446"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          483 a          813 c          854 g          450 t
ORIGIN      
        1 cgctctggtg atatcggact gcaggcggat aaggagcgcc gccgcttcgg ggtcggtcac
       61 cggggtgtcc ggctccggag ccggccgctg tacatccgcc atcccaccag cccggccagg
      121 atggcgacga cgatcccaac caggagaccg atgccgaggg tcgggtcctg ccggtagacg
      181 tcaaggagga cgtcggcgcc gagggcgatg atgaggctgg agacgatcga cccgatgcag
      241 acgcaccaga agacccgaaa cttctccagc ccgagcagcc ggccgaggat cgccccgttc
      301 atcgccccgg taccctgaag cgggaagaag atgaagagga tgagcccgat tgtcgagaag
      361 tgccggagcc agggcagagt ctcggtgtag ttccggccca tcgtcatccc gctctcgagc
      421 acccgcccga cgagcgggat cttcagcgcc aggtcgaagt tccagacgac gaagagggag
      481 acggccacgt caaggaggaa gagcgcgagc gtgatgagcc accaggggta gcccatcagg
      541 atggcgatgg ggatgatcga ctccttcccg gccgggggga cgaagtaggc ggcgagcaga
      601 ccggagatga tgaggaactg ctggtagggc tcggtcaggt agagcgccgc gaagatggcc
      661 gggatgatgg cgagcgggag gatgaatttt atggagccgg tgaggtaggg atttgcgaga
      721 agggtcttcc agccttccgg tccgggtgta ctggtagtat ggtccgcggt catgggtcga
      781 tctctggatg gggagatact cagttcaggt ttggggtttc cggataaata cgttctctgc
      841 gcgggttcca gcaggttcaa atttatgcag tgcaaggata ggtagcatga ccccttcccc
      901 cgtcaccagg gcaccccgca tcctccccgc cgtcgagagc ggcacccggg agagcgcttt
      961 atgcacgatc gcgaatgtcg ggaccgcgct cgaatccctg cgcgaggtga gaaagcatgt
     1021 ccgcggcgac cacaagcgcc gcctcgacga cgtcgcggtg gtcctccggg tggtggcgct
     1081 cgaggcccag gcgacctacg cgatcaccga cgacgaggcc cgggcgttta tccggaactg
     1141 ccgctcacgg taaggtcgcc gggcagcacc ggctctgtga ggagacggta cccctcgctc
     1201 agggccaccg ggtaggcctg gaactcctcg atgaaccgga gacccccgga accgtccaca
     1261 cgtggtttca cgggatatcc cgccgtcgag gtgctcccgg ctgcttaacc ggcatgatct
     1321 gcattcagtc ttccccggat cctcttgcag aggatttaac cggttccgcg ccaacctctg
     1381 gagtaatgaa cgagtcgctc cggcaggtcg tccacctcgt cttcggcctc gggattgcgg
     1441 gttttatcct cctcttcgac cgcgacgtta ccctccccgc cctcgtgctc gctctctttg
     1501 tgggcttcat actctccgac gctatctccc ggggctatac catcccgctc atctcggcgg
     1561 tcatcgcgag ccttgagcgg cgggacgctg ccccgggccg ggggaccctc ttcttcgcac
     1621 tcggagccct cttctgcctc gtcttcttcc caagagaggt cgccttcatc gggctcgtgg
     1681 tgctctcgct cctcgacagc atcaccaccc tcgtcggcgt ccgctttggc aggaaccgga
     1741 tctacaacca caaatccttc gaaggcacgt tcgccggggt ggtggtgacg gcggctgccc
     1801 tctgtctcct cgtcccgccg gcgaccgccc tcgtgacggc cgcggtcacg gcactcgccg
     1861 aactggtgag ccccgtggac gacaacctca tcatcccggt cgcggcctgc ctcgtcctca
     1921 cggtgctgct ctgagatcgt tgaaccgccc ggttcaatta ttgtataaag gatcctgaat
     1981 ttcttcttca cagaggtgaa gaacgatgaa acgcatcatt gcagtagtca tgatggtcct
     2041 cctcgtcttc ccggggttcg cggccgccgc ttccggggga gagcagacgg gggatggaga
     2101 ggggaatccg gctgccggag accgccagct ggtgagcaca gaagagaacg agacttccag
     2161 ggaccaggtg cagcagcgga tcgcggagaa cacaacgccc cggggagaag acttcgcccc
     2221 tggccgacaa cgggtgagga cagaggagaa cgagacctcc ggggaccggg tgcagcagcg
     2281 ggtcgcggaa aatacgaccc ccgcggggaa agagttcacg cccgaccgaa accgggtccg
     2341 gcttgccgtc cacgccctgc tcgccgctga aaaccggacc ggcggcatcg ggcagaacgt
     2401 ctctgccatc gcccgggagg ttaacaactc ggtcgagaag acgctcgcgg ccgaggagca
     2461 gatccggacg cggcatagtt tcatgcgttt cctctttggg ggcgacgctg aagccgcccg
     2521 gttgatcgag ggcgaggcgc agctgaaccg ggaacgggtc acagaactcc ggcagtcgat
     2581 cgagaactgc acctgcgatg
//
LOCUS       DAJB01000098            3054 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_138157, whole genome shotgun sequence.
ACCESSION   DAJB01000098 DAJB01000000
VERSION     DAJB01000098.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_138157"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          534 a         1007 c         1032 g          481 t
ORIGIN      
        1 catctacaac ttctcggtcg ccgggtgcca cttctgccgg accgaacgcc ggcggctggt
       61 cacgttcgtc ggcgccgccg ccgggaccct gctcgccctc ctcggcatgt acgactggct
      121 gatcccctac atggtcgcga tgggggtgct catccccccg ctcggcgggg tgatcatggc
      181 cgacttcttt gtccgccacc gggggcgcta ccccgcgctc ggggaggcaa tcgtcccacg
      241 gttcaaccgg caaggactcg gggcttacgc cgccggctcc ctcgccgccc tcgtgatccc
      301 gggcattccc ccggtgaacg ggatcgtcgc cgcattcgtc gtctatgcgt gcctcgcccg
      361 gctcacatga accgctcgaa ccggtcgcgg ggcacccggc agatcgggca ggtctcgggc
      421 ggctcgctcc gcgcgcagag gtagccgcag acccggcatc gccagatcgg atacttcagg
      481 gtacccgggg ccgcgccggc cgtggcacgc tcctctttct gccgccgttg ctccccgggg
      541 ggtggccgcc gttccggaac ggggtcggcg gcacgcaccc cggcctccac gtcggcggtg
      601 acgtagaggg cgcagtagca ggcgccgtag tcggcgagat cggggtcccg gtagtcacag
      661 gggcagatga tgtcgagatc gtcctcccgg ttccccgagg caagccggca ggggcaggag
      721 atgtagccgt aacgctcccg gttcgcgagc aggccgcgga cgaggtcccg ggtgaactcc
      781 cggtccgggt tgaggtgata accgccggcc tccgcctcgc ggtcgaggtc gcgcacgaaa
      841 cggtcaacct ctccgtcgct tgccgcgtca ggcaccgacc gcctcccgga tctcattctc
      901 ccggaacccc acgatcacct tcgcgtcatc gatgacaacc gttggaaacg agagccgcgg
      961 gttccagcgt tctacctcct ggaccacatg gtcacgctcc tcacccgtga gcaggtccac
     1021 gtagacgtac gagaacccga cgccgaggtt ggtgaggagt tctttcgtcc tggcacacca
     1081 gccgcaggtg ctcagggcat agaggatcac cctgccgcgg tcaactccag gcacatgctg
     1141 catctccgtc aaacgacttc ctccttcagg atcgccccgg ttcgggggcc gaaggtaagt
     1201 agtttacccc ggctccggat caggaggggg cggacctgaa gaggagaata aggccggcac
     1261 cgtccccgga gatcgccagc gacgccccac agagcccgaa cttccgccgg tagaactgga
     1321 gcctcttcgc agagaggacg ccggaaccct gaatcagaag cccgaccgca tccccctccc
     1381 ggcgggggga gagccggatc tcccgggcac cggcggccgc catatcctcg agaacgccga
     1441 cgacctcgtc ggagaaccgt tcctggtcga tcagtaccgg cgggagagta tctccggcgt
     1501 tgatagagag gtcaatcccc tcaaagagcg gaaggtaggc aagccgggag acgagttccg
     1561 cacggtatgc cgccgggtct tccgccgtct cgataaacgc ctcgtccgag aacggcggag
     1621 tcaagacccc ggcaaccagg tcgcggagca ccggcgggag agcggcggcg gagagatgcg
     1681 cggagtcccg gtagccaaag atcgtgttca tgaagtcggt gagcaggcgg ccggcccggc
     1741 ggaggagcga cgcgtcgacg acgtggtcca gccggtcccc cgcaaaatgg acctcgatcc
     1801 gctggatcac ctggacggcc ttgaggacga gctggacctc gaacctctcc ccggcgtgga
     1861 attcgtcggc aaaccgtgag aaatcggcaa gaaacccatc gatctggtcg gactcaagca
     1921 gttcctggta acgccgggcc tcttctgcct cgccgagttc gtcgaggcgg taggcgaggg
     1981 cgtagaggcg tcccgcgatc accgcaaaga gccggttggc ttcctgaagc aggtcgacag
     2041 cgtgctccag ggagtcatcg agccgctccc ggtcgaaggc accgatgccg gagagcccct
     2101 ccgcctcccc ctcgagccgg tcgaggagcc ccggcatcgt cgtggccggg atggtcctgc
     2161 cgtggcccgg gcagcagagg ctcaccggtt cacgggcgag gatccaccgg acccgttcaa
     2221 tcgacacgag cagcgccggc tggtcccagc cggcaagccc ggcgatcccg gggctcgccg
     2281 caaagaggag atcgccgatg aagaggaggc cgccgaagtg gatgcagatg ctgtcgggtg
     2341 aatgccccgg ggtgtggtag accgtaaggg catccccgga gcggagcagg atctcctgcc
     2401 ggtaaagggt gatgccgccg tctgtctcca gggtctcgcg gtcgcgagcg gtgaggagag
     2461 ggaggccgac cgggagcggc tcgacctccc ttcccatcag ttccgcaatc gtccggcacg
     2521 ggtcgcccgc ttcaagcgcc cgggcgcccg cctcctctac agccacggtg aggtcaggga
     2581 gagaccggaa ccgccggtcc cgggttgcct gatagcagtg gtcggcgtgg cagtgggtga
     2641 ggacgaggag gaccggccgc ggggttcccc tgaggaggtc gtccacgagt gccatgatcc
     2701 ggtccatctg cccggcgtcc gccccggtat cgatgagcag gatctcgccg ggcgtccgga
     2761 tgaggtaggc gttcgaacag gtaacatcag gtttcctgag gaaggggtag atctcggcgc
     2821 cggtggttcc cggcaccggt tgccattgtt ggtcttcaag cattgtctgc acccggctaa
     2881 agaggaataa cagaccattg ttgcaccaca aaggagaaaa atcaggtgga tttccccgga
     2941 agccggactg tccggccgga tgtgatcctg cccggatacc ttcttatccc gccgtgcagg
     3001 aagaagaggt gaggtggaag gaggatgtca actacccccg gttaaaaccg gggg
//
LOCUS       DAJB01000099           24178 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_37510, whole genome shotgun sequence.
ACCESSION   DAJB01000099 DAJB01000000
VERSION     DAJB01000099.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 24178)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24178)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24178
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_37510"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    11692..11720
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4568 a         7729 c         7345 g         4507 t
ORIGIN      
        1 gatccgaccc atatttatac atggcctctc acgtccaccg gatagaggtg cactataccc
       61 gcgatccacg gctacagacg cgtaccgaga ggttccgctc gctgggcttc cccctgcgcg
      121 agctgcatct cgtcgatgtc tataccatag cgaccgctca gagggacttt tcccctgatg
      181 agctctcgca gatcggtgcg cagctcagca accccgttgt ccagcattac tccgtagacc
      241 ggccgacgga agccaccttt gattatgcaa tcgaggtcgg gttcctgccc ggggtcaccg
      301 acaacgccgg gacgacggca aagcagaccc tcgaggacta cttcgggttc cggttcggcg
      361 agggggaggc ggtcttctca tcgcagctct acctcgcgag cggcgacctg acccccgaag
      421 ccctgcagag gcttacaacg gctcttgcaa acccgctcgt caaccgcgtc cacgtcaaga
      481 gccgcgagga gtatggcgag aggggaatgg acccgatcgc gccctttgtc agcctccacg
      541 agctgcaggc cgccggggcc gtggacctcg acctctccga cgcggacctc gcacgtatcg
      601 ggaaagaggg gatcgtcgac cccgagacag aggagcggag ggggccgctt gccctcgacc
      661 tcgcccagct ccacgcgata cgggactact tctggaggct cgggcggagg ccgacggacg
      721 tcgagctcga gtctctcgcc cagacctgga gcgagcactg caagcacacg atctttgcat
      781 cggccatgga tgacgacgtc ccgcgagggc tttataagtc gtgcatccag gccgccacca
      841 acacgatccg caaggagaaa ggagatgacg acatctgcgt ctcggtcttc accgacaact
      901 ccggcgccat catcttcgac gacgagcatc tcgtgacctg caaggtcgag acgcacaact
      961 ccccctcggc actcgacccc ttcggcgggg ccctcaccgg catcgtcggg gtgaaccgcg
     1021 acacgatcgg gttcggcctc ggggccaaac cctgcatcaa cgtctacggc tactgcgtcg
     1081 gggaccccga gaccgaaccg gccctcttcc gcgggaagaa ccgggaaaac cccatcctct
     1141 ccccccagag gatcttcgaa ggcgtggtcc gcggcgtggg cgtcggaggc aactgctccg
     1201 gcatcccgac accgcagggg tggtgctact tccacgaccg ctacgtgggt aagcccctcg
     1261 tctttgcggg cacggtcggc gtgatgccgc gcttccgtaa cggaaaactg ctccacgaga
     1321 agcaggccct cccgggcgac ctcgtcgtca tggtcggcgg cagggtcggc aaggacggga
     1381 tccacggggc caccttctcc tcggaggctc tcgacccggc cagcccggtg accgcggtcc
     1441 agatcggcga cccgatcacg cagaagaagt tctccgacgt catcgtcaag gaggcccgcg
     1501 acctcgacct ctaccggagc atcaccgaca acggggcggg agggctctcc tgctccgtcg
     1561 cggagatggc gaaggagtcg ggcgggtgcc acgtcctcct cgaccgcgtg ccgctgaagt
     1621 atcccggcat ggcgccgtgg gagatctgga tctccgagtc gcaggaacgg atgacgcttg
     1681 ccgtccccga ggagaaggca gacgcgttca tggacctgat gaagaagagg gaggtcgagg
     1741 ctaccgtgat cgggacgttc accgataccg gcaggtgcgt cgtggagtac aacggcgcca
     1801 cggtcatgga tatcgacctc gatttcctcc acgacggctt accgaagaag catctcgtga
     1861 cgaagcccgt ggagacgacc tatcccgagc ccgatttcac gtgccctgag aggctcgacg
     1921 acctcctcct tgccatgctc aggaggaaga atgtctgctc gaaagagttc atctcgacgc
     1981 agtacgacca taccgttcag ggagggcacg tgctcgggcc cgtgcagggg agcgggcggg
     2041 tccaggcccc ggccacgctc acgaagatcg tgcccggctc ggtgaagggc gtggggctct
     2101 cgcagggcct cttcccgtca tacacggaac tcgaccccta ccgcatggcg gttgccgcga
     2161 tcgacacggc gatccggggc ctggttgcga taggcgtccc gctggagacg atcgcgctcc
     2221 tcgacaactt ctgctggtgc tcctccgacg agcccgggcg gctctggcag ctcaagcgcg
     2281 ctgcgttcgg gtgccgtgac ggtgcggttg cgtttagaac accgttcata tcaggcaaag
     2341 acagcatgtt caacgacttc tccgggttcg acgcggaatc gaaccccgtg aaggtatccg
     2401 cactcccgac gctcctcatc tcctcgatag gcgtccaccc cgatgtcgcc gccgcggtat
     2461 cgatggatgc gaagttcgac ggcgacctcg tctacgtcgt gggggagacc ggagaggagt
     2521 taggtgggtc ggagtacaac gcgctcctcg gcatccaggg aggtgcggtc ccgtccctcg
     2581 aaatcgagac ggcaaaggtg cgatacggca ggatgaccga tgccatcgag aaaaaactcg
     2641 tcgcctccgc cttcccggtg acccacggag ggctcctcgt cgcccttgca aaggtcgcga
     2701 tcgccggggg agtcgggatg gagatcgcca tccccgataa gatgtccccg gaccgctacc
     2761 tcttctcgga gtcgctcgga cggttcgtcg tcacgatcgc cccctccgac cgggaggcgt
     2821 tcgaggagat cttcggcacc gacgcacacc tcctcggccg cgtcgacggc acggtcttcc
     2881 gggtatcggc aggaaaaact ctcatcgaac agccggtcac ggcgatggag gctgcatata
     2941 aagcgccttt tggagggtac tgaaatggct cgcgcaaaca gtgccagagc cctcatcatg
     3001 agcggctacg ggatcaactc cgagatggag acgcaggaag ccctgctgcg ggccggcatg
     3061 gcgtctgata tcgtccacat caacgacctc atcgcggggg agagggccct ctccgactac
     3121 cggctgatgg tctttcccgg tggattctcc tacggcgatg acaccggcgc cggcaacgcc
     3181 tacgcgaacc gcgtccgaaa caacctctgg agcgacgtaa aagagttcct cgcgggagat
     3241 aacctcgtcc tcgggatctg caacgggttc cagatcctgg ccaatctcgg cctcgtcccg
     3301 gcgttcgacc gggactatcc ccgcgacatc gccctgatgc cgaacaagaa aggtgtgctg
     3361 gagtgccgtt tcgtcacgct gaagcctgcg gcagacaacc tctggacgag gggtatttcg
     3421 cacctctact gcccggtatc ccacggcgaa gggaatttct cctgttcgga agagacccta
     3481 aagcacatcg agcgcgagaa tatggttgct ttccggtact gccgggacga tctctccccc
     3541 gcgggagggg agtatcccta caaccccaac ggctcgctcg cggatatcgc cgggataacc
     3601 tcggcggacg gcaaggttct cgggctgatg ccgcaccccg agcgggccat ggagttcgtg
     3661 aacctctacg actggccgac ccagaaagag cggctgaagc gcgagggaaa acccttgccc
     3721 ctcgaatcga tgaatatgca actcttccgg aatatcgtcg cgtacttcgg ggaataaccc
     3781 gaccgaagta ttatagaaga gattaccctt ttcgagaggg ggaggcgtgc cgggaaccgg
     3841 cagcctctaa agagtcgtgg gctccactgc ggcgggagcc cgtttcctcc gtcccggtct
     3901 ctcctcacac cgagaacaac tgcccgagtt ccaccttctg ccagtcgcgg tccacgacct
     3961 tcatccagag ggtacgggtc tccgggacaa agaccacctg gtgatccgag gagtacgcca
     4021 taccttcgag catgctgtgc cggaaggtcg cacccccgtc ctggatgagc acatcgcgga
     4081 tagcaaccat ccgctccccg tcgatcgatc cccggttctc ttcggcgagg cggaggaggc
     4141 tggcataccg cgtggccgag tgctccgccg ggggcgccgc aagccgccac gacgggtcca
     4201 cgaagtggtt cgcggccgcg atcacgcccg ggccctcgcg gcgcttgata tcgtagacgg
     4261 tctcctcgta ggagtaggcc cggtccggtc cggccgcgtt cacgatccat gcgcagtccg
     4321 cgcgagtggt catgatcccg gcatcgagcc cttccatgtc ggagtagtcg agcatcagac
     4381 ggaagaactc cccgaccagg tcgggcctgc cggcgatctc aagggtactg ccggagtccg
     4441 ccgcgttatc gtcggcgatg aagagccctg cgctgttcat cagggtctcg gggcgtgccc
     4501 ctgccgggcc gaaggtcgcg acaccgttgc tcccgtcggt cgggttgtag accgcgaggg
     4561 tgtagtaggg gtagaagacc gagaagacgt caggcaggtc gtagttccgg gagaggacca
     4621 ccgagccgtc cggggtgtag ttgccccata ccgccacaaa agagcaccct gccttcgcgc
     4681 cgtaggtcac caggtagacg atcggtccct cgtagaggac atcgacctcc tctgcggaaa
     4741 gaccggacgt ctccgcgatg cctgcccgga tctccttcat gcgcttcgac tgaagcgccg
     4801 ttgccgtccg ggcgaactcg ccgacttctg ccccggtgta ccccgatgcc gtgaaatggg
     4861 tgcggagcat cgcgtactcg gcctggagtt cgtctttcat cagggcgccg tactgccgcc
     4921 ccatctcccg gtaggtgccg gagaggacca cgacgtgata cgatcctgcc tcgtagcgcg
     4981 tgccgttgcc gaacacggca acctgcgtgg gtccggtatc ggcagccccc gcattctccg
     5041 aagagaggac gaccgccgtc gcgggaacca cgagcgccag tgcaataagg aggtagagta
     5101 gtttcacgaa gaagcatctt tgccgggatg gataaatctg ctgaaataag ttgtatgcgc
     5161 ctgtttttca agttgcgagc agtccgaccg ctgtaccaac tctcatatcc cggcacagca
     5221 taacaactat attatcctcc cgggagaata tcccggccgg aacaccactc aaacggagga
     5281 gtcaccacga agatcagggt cgcccgctgt tttgcagaag agagcgccga tacaccggcg
     5341 gtcctggagg tcgaggtgga ccagggggac gtcccttccg cagacgaaat cctgacgttt
     5401 gcctccattc acggcgactt cgtctgtatc tccgaccgca tcttccgccg gttcgagatc
     5461 atcctccccc cggagagccg cctcgaggtc ctcaaagacg cgcttcttga ggtcgtgcac
     5521 ctcgagcgtg aaggctccgg gggcgggggg ctccatccct acgaggtcga agacttcatc
     5581 gatgcgctcg agcggacgcg gcagaagtta ctgggcgacg ccccttgata ccccgctccg
     5641 gcagggcccg gcctgaccgg agggtcgtcg ccggcacctc gggcatgccg tgcagcaccg
     5701 gtgccgtcca ccctcaccgt tcgttcatct ttttgcccgg gaccgcgaac ctgtagatcg
     5761 agtggcagaa gaaacgagag cggcgcccgg acccggtggg cttgccgtca acaccatcca
     5821 taccatggac tgcatccggg gcatgcagcg cctcccggag ggctcggtcg acgtcatcgt
     5881 cacgtcgccg ccgtacaata tcgggaagga ttacaactcc tacaacgacc ggcagccccg
     5941 ggaagactac cttgactgga tcggggaggt cgccgccgga gcggcgcgtg tcctcgccga
     6001 cgacggctcg ttcttcctca acatcggcgg caaaccccgg gacccctgga tacccttcga
     6061 cgttctccag cggttccgcc ctcacttcga gctgcagaac gtcatccact gggtcaagtc
     6121 gatcgcaatc gaaaaggagg acgtgggcag ttacgagaat atcgccggag acatcgccgt
     6181 cggccactac cagccggtga acagttcccg ttacctgagc cagtgccacg agcatatctt
     6241 ccacttcacg aagaagggcg aggtggcgct cgacaaactg ggtatcggcg tgccctatca
     6301 ggacaagagc aacatcgggc ggtggaaggc ggcggagcgc gacctcaggg accgggggaa
     6361 cacctggttc atcccctacc ggaccatacg ctcctcccga ccccatccga ccagtttccc
     6421 ggagaaactc cccgagatgt gcatccggct ccacggctgc cggcccggga cgctggttct
     6481 cgaccccttc atgggcatcg ggagcacggc gcttgcggcc ctcgccctcg gcgcggacta
     6541 catcgggttt gagatcgacc cggactacag ggcaattgca gaggcccgga tcgccgaagc
     6601 atgccgcgaa aaagaacaga cctgacggat attatatccc aaaacccaaa acgagagact
     6661 gatggatacc ctcgtcccct tcgccgttgc cctcgtggtg atcaccgtcg catcgctcag
     6721 gtatcacctc cccccgttcc tcaccctggt cgggacatcc gtgctcttcg gcctccttgc
     6781 cggcatgccg gtggagacac tcatgccggc gatcacgggc ggtgccggga ggatcttctc
     6841 catcctcggg atcgtcatct tctgcggggt cggcattgcc caggtcctcc gtgagagcgg
     6901 ccggatagcg gatatcgtcg ccgatatccg ggggctggtg cgccgccccc tcggcacggc
     6961 gggagcggcc ggctacctcc tctccgtccc gctgatgtgc ggcatcacct cgttcgtcat
     7021 cctcgccccc attctcaccc acctgcgccc cgacccgcag gcatcaaaaa tcctcctcta
     7081 ctccgccggc gtcgccggga tgatatccta cgtgctcctc tatcccgccc ccgtggttta
     7141 cgccaccgtg acggcgctcg agatcttctc cggtgagccc tggactatcg atctcgtcgc
     7201 cctcccggtc tctctccttc tcctcgccgg gttcctcctc gtcctccggg cccgcacgct
     7261 gccggaggcg ccgcctgcag aacccgctcc tcctgcgggc ggccgaaatc tgcgtgcctg
     7321 gctgccgttc ctcgtcatcc tgctcatgct cgcgatcggg tctctggtcc ccccgctcca
     7381 cgctcttgcg aacatcaacc tggcgctcct cgccggactc tttgccgctc ttgccaccgt
     7441 ttcggacgac acccgacaga aggcccttgc ccgggggaca aagaacgccg ggatcatcat
     7501 ctttgacctc gccggtgccg gggcgttcgg cggcgtcatc gccgcaagcg cgttccctgc
     7561 agacgtgact gcgctggtcc tgggctttct tcccatcacg ctcctcccgt tcgtcatcgc
     7621 cgccctggtg caggcggcac aggggtcacg ggtcgttacc gcaaccgtca ccgccacgat
     7681 cctcgccacg atcccggccg ttgcggcgat cgacccgctc gccctggtgc tcatgatctc
     7741 cgcgggggcg ttcatgttct cctacgtgag cgaccccttc ttctggctgc tcaaacggac
     7801 gaccggcgac gacttctccg cggtggtgcg gaactacacc ctgccgctct cggcggcagg
     7861 cctcgtcacg ctcgcggttg cgctggtgat tcaggcaggt ctataaggcg tttttgtgcc
     7921 gcgtcccact ggtattcctc aaaatctctg ccccggccat gcatattata taacacgggc
     7981 ggtatgtagt ccctgccaga gggagaatga tggatattaa gtacgtatcg acgacatgcc
     8041 cgtactgcgg caccgggtgc gggttcaacc ttgttgtccg ggacggcaag gtctttgacg
     8101 tggcgcactg gcagcgcgcc cccgtcaacg ggggcaaact ctgcccgaaa ggacgctacg
     8161 cgcacgaatt cgtgaacagt cccgaccgcc tcacaaagcc gctcatcaag aaggacggca
     8221 agtttgtcga ggcgacctgg gacgaggcct acgacctgat cgcggagaag ttcaggtcct
     8281 acaaacccga cgagatagcc tgcctctcct ccgcccggac atcgaacgag gagaactacc
     8341 tgatgcagaa gtttgcgcgg gtggcgctca agaccccgca tgtcgaccat tgcgcccggc
     8401 tctgccactc atccaccgtc gcgggcctcg cggcggtctt cgggtccggc gcgatgacca
     8461 actcgatcat ggacattagc gagtcgaaat gcgtcttcgt cctcgggtcc aacaccttcg
     8521 agcagcaccc gctgatcgcg cgaagcatca tccgtgcgag ggagggcggc gcaaaggtgg
     8581 tcgtggccga cccgcgcaac accccgaccg caaagcaggc cgacctctac atgccgttcg
     8641 tctccgggac cgacgtcgct atcttaaacg gcctgatgca ggagatcatc aaaaacggct
     8701 gggaggataa ggagttcatc gcgaaccgga cgaaggactt cgagaaactc aaggaagtag
     8761 tcatgaagga tacctacagc cttgaaaacg tctcgaagac atccggcatc ccggtcgaga
     8821 gtctcaggac cgccgccgag tggatcgcaa aggccgatgc tgccgcgctc atctactcga
     8881 tgggcatcac ccagcacacc gtcggcgtgg acaacgtcaa atcgacagcc aacctgatga
     8941 tgctcaccgg caacctgggc aagcccggca gcggcgtgaa cccgctccgt ggccagaaca
     9001 acgtgcaggg cgcctgcgat atgggagccc tcccgaacgt ctactccggt taccagaagg
     9061 tcaccgacga ggcggcccaa aagaagatga aggagatgtg gggcgtcgac aagatcgccg
     9121 agggcagggt cggttacacc gtcaccgaga tggtcaacct cctcgccgac aggcccggca
     9181 agctcaagtg catgttcatc atgggcgaga acccgatgct ctccgacccg gatctccacc
     9241 acgtcgagga agggctcagg aacctcgagt tcctggtcgt gcaggacatc ttcatgaccg
     9301 agacctcccg gcttgccgac gtcgttctgc ctgcggcatg ctacgcggag aaggacggca
     9361 cccagacgaa caccgagcgg cgcgtccagc gctggaagaa ggcccaggac ccaccgggcg
     9421 aggcgaagcc cgactggcag atcctctgcg agctcgggag ccggatgggc tacccggagc
     9481 agttcgcatt caacagccca gaagaagtct tcaacgaagt cgccgccgtc accccgtcct
     9541 accacggtat atcctacggc cgcctcgacc ctgacggcct gcactggccc tgcccgaccg
     9601 aagatcaccc gggaacaccg atcctccaca gggagaagtt cgccatgccc gacgggctcg
     9661 gtgtcttctc cccgatcgag tggaaaccgc ccgccgaggt gccggacgcc gagtacccct
     9721 atgtgctcac gacgggccgt acgttatggc agtggcacac cggcaccatg acccgccggt
     9781 cgtggagcct cgagaaagag gcgcccacgg gctggatcga gatcaacacc gaggatgcga
     9841 aagaactcgg gatcgaagac aaagaggtcg tccgggcgag cacccgccgc gggtccatcg
     9901 atattcccgc acgggttact ccggagatca tcaggggtgt catgttcgtt ccgttccatt
     9961 acaaggagtg cgcggcgaac ctcctgactc acaacgcgct cgacccgata gcgaagatcc
    10021 ccgagtacaa ggcatgtgct gtaaaagtcg agaagattgc ggaggtgtga gatatgtcag
    10081 caaaaggcga tttactctat gcgtggacga cggacgacaa cctccgggag aaggcggaga
    10141 ccggcggtgc ggtcaccgcg ctgctgcgcc acgcccttga gagcggtatg gtcgacgggg
    10201 tctttgcggt ccggaagggt gcggacgtct atgacgcggt gccggccctg atcaccgacc
    10261 ctgccgagat cggtggcatc gcggggtcgc tccactgcgg caccctgctg ctccccaaac
    10321 agatgcggcg gtgcctcctc gccaccgagc ctgatatgaa gatcgccacc gtcctgaaag
    10381 gctgcgacgt caaggcgatg tacgagatgg ccaagcgcaa ccaggtcaac ctggacaaca
    10441 tcatcatcat cggcttgaac tgcggcggca ccatccggcc ggaggtagca cggatcatcg
    10501 tccgggagaa gctcggtctt gaccccgacg atgtcgtcaa ggaagagatc gacaaaggaa
    10561 agttcattgt ccagacaaaa gacggccagc atgcctccat ctcgatcgac gaacttgaag
    10621 aaggatcgga agacctcctc gggaacgagg ggctcggccg tcggtccaac tgccgccggt
    10681 gcaagatcaa gattccgcgg caggcggatc tcgcctgcgg gaactggggg gtcatcggcg
    10741 agaaggccgg aaacgccacg ttcgtcgagg tctgctcgga aaaaggtgcg aacctcctca
    10801 atgcggccgc aaaagccggg gcggtcgcca ccgaacccgc gaacccgaaa ggtgtcgaga
    10861 tccgcggcaa ggtcgagaac gcgatgctga aactcggcga caaatggcgg gcgcgctact
    10921 tcggagcgct cggtgagggc acggagcgcc tgaacaagat ccgggagcag acctcccggt
    10981 gcatcaagtg ttactcctgc atcgagaact gccccatctg ctactgcatc gactgcagcg
    11041 caaagaaaga ttatctggtt gaaccgggcg tgatcccacc gcccttcatg ttccacctga
    11101 tccggttcgc gcacatctcc gactcctgcg tcaactgcgg ccagtgcgag gaactctgtc
    11161 cggtggagat atcgaattcg gtcttcctcc atgcgatcca gaccgatctc gagagactct
    11221 tcgggttcca cccgggcgag gatatgaccc cgccggtgct cgcgctcgtc gaggagagct
    11281 cggaacgcga ccggctcatc gccaccggga gcgaccagat cttcgatatc ttcagatgac
    11341 cggagatcaa ccgtcaacca tcgatccctt ttttctgtca gggctgtttt ccggttcaat
    11401 cagggtgctg ttcccggtaa ggcggcgttt tccccccaac tgcgacgctc tcctgacata
    11461 ggccttcatg acgtccgttc gtgcgacaaa tcccgagagc gccctcgggt cctccgccgg
    11521 gacaacgggc aggtgatgga tatcgtgttc ggtcatcaga tccagagcct cacgtagcgt
    11581 acaggcagga tgcacggtga acacccgtga actcatgacc ttctccaccg gtacgtcgtg
    11641 ctcgccattc atccggtgat ctctgacatc atcgagagcg ataatgccga cnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn cacccgcccg gtggtgtcat cgggcgatac ggcgacgata
    11761 cggtcacggg gcaccataat gtcgcccaca tgaatatcct ggaggatttc aaccatatac
    11821 tcgtcccggt gagcagccga ccgggaccgg ttcagcacct gctcgcggaa gatggtagac
    11881 tgaccgatga gaaggaccgc cacggagacg gcacccattg ccggcacgag cagggagaaa
    11941 tcgccggtca tctccacgac catgatcatg accgcgatcg gcgcgtggga gattgcaccg
    12001 aagagagcga tcatcccgac gacaacgaag accggcaccg attcgacggg cacgatcccc
    12061 gggagggcga ggtgcagcaa ggacccgaac gcgccccccg tcgccccgcc gatcaccagt
    12121 cccggcgcga agacgccgcc gcttcctccg gagccgatgg tgagtgacgt cgtcaggatc
    12181 tttgcaaatg ggaggaagag cagcactccg atcgggagca tgttatagag cgcgagctgg
    12241 gtaaagccgt atccggtgcc gaggctgccg aggccgacga ttgccgcttc gggagagaga
    12301 gccgcaagag caagaacgaa cgtgccggtg aggaatgccc cggcaagggg tctgaagtga
    12361 tttggaagat tgaaacggct gaatattcct ttgaaaacct tgtttgcccc atagaaggtg
    12421 tggatgtaca gcaggccgac tgctgtcgag accacgccga ggaggatgaa gagcggaatc
    12481 tgggtgacgt tccagaagac tactccgggg ccgaagaccg gttcgaagcc ttcaaccaga
    12541 ccgaagatgg cgtatccgat gaccgaggcg aggaacgcgg gaacgattgc ttcggcttcg
    12601 aagtcctgtt tgtagaggat ctccgcagcg agaattgccc ccccgagagg cgccatgaag
    12661 atcgtgccga ttccggcgcc gacaccggtg gcgatggcaa gcctccgctc acgggcggag
    12721 aggcctaaca gatcggcggc gatcgagccg aaaccggccg acatctgtgc agtcggccct
    12781 tcacgcccgg cgctcccccc cgttgagatg gtcaggacgg cagtgaccgc tttgacgagg
    12841 gggacgcgcc agcgaacccg tccccctccg tgaaacgcct ttatcgccgc gtcggtcccg
    12901 tggccttccg cctccggggc aagggtgtag atcaggaggc cggagaggag tcccccgaaa
    12961 cagatgacgg ggagtatcag ccagaggtgc tcgggaggag cccactgact gatatcctgg
    13021 agtgtctgcc cttccttcgg gaggtggaac ccgacgatat ctcccatgaa aaacgcagtt
    13081 cctagtttca gcccttcgaa aaagaacagt gcaccgatgc cggcgatgag cccgacaatg
    13141 acggcgataa gtatgatcct cctgtaaggg gtgacggttt cctcgatctg catgggatat
    13201 atgaggtatg gttgcttaac acatatttaa tttggttagc ttcttgcagc agtatacgat
    13261 acaggggtgc gcatcgccgc cggacgtccc ggcgcgccct cttcttttat cgtgagaacc
    13321 gggcgataac tgcgtagggg acgattttct tcaaaggggg cactgggggc cacccgatca
    13381 tcgccaccgg atcttcgatg tgttgcggtg acgctccgca gtgcgacgga tgggacgtcc
    13441 ggagcttgag caccagaggt gtccatccgg tcggggtgct ttccccactt ccctgtgtgc
    13501 ccgttcccgg agccgccgca tgactcccgg gagatgcctg acaacgccca tgtaacctcc
    13561 ggtgtcccgg gatcgggatt cggctgctgg agcgggatcg cagccgtcag aggttgagca
    13621 tatgcgtctc atcaaggttg cagcatcggc gggggttcgt gacggggcta taccgaagac
    13681 cgtcctccag gataaaaggt gcgcccggtc gccgcttgct gcggcaccat gacagacccc
    13741 ggtgatcgtg gccgaactgc agccgggctc ctttacctgc cgctccgcct gccgcgccgg
    13801 gttccggtgc ggccgatccc cggcgagacc gggcgcgaag actggcaggg accgcaaaga
    13861 ggatacctca ggcacccata gaccgttcga tggggaatct gacccggaac cggagatgac
    13921 ccccaaaaac tgaaacgagt gcgtaacgtc tccctaaaag cacccttctt cccgtaagaa
    13981 agcctgtttt gggaacacgt cccgacgcgc agacctctac ggtgatatta ccggttattt
    14041 gccgtcgcgg aacaaatatc gtcggagacc agatgatcga cgcggcagct tccatatacc
    14101 tgaagaaata tctttccaaa accacccata ttccgcgtgg tcccaaaagc atttttaaca
    14161 gcatcgttta cctatttgtg aagtttaatt gcaggaatga acttacagcg gcgataaatg
    14221 cctcaatccc ggttatattc cccgcgcata gcgatcattt cgaaacttat taatagcact
    14281 ctcagattac tgattaatta gacctatcca ttcagattac ttgatggtat aggtaggacg
    14341 tttagagagg tgtatgaaaa atggtattcc atcctccagt tgctatcgta gcaaaagcag
    14401 gtgatgccgg gaagtacaag accggtctgc cggcctggaa catgatcctt cgtggattca
    14461 tggcaggtgc atatattgcc atgggcggtg cccttgcaac ggtctgcagt accggtgtgg
    14521 cgacattcct cggtgcgggt ttcgcaaagc tgattctcgg tgctgtcttc cccgtggggc
    14581 ttattatcat cgttctgacc ggtgcggaac tcttcaccgg cgatgcgatg ctcgccccga
    14641 tggcagcctt catccacaag gtcagctggg caagcgtact caacctctgg ctctgggtat
    14701 acatcggcaa ccttatcggc tcgatcttgt ttgcgtatat catggccaat ggcccgttga
    14761 caaacttcag tgcagcggga gctccgaccg ttacagcttt cggtctcagt gccgtgaata
    14821 tcgcgatcgg gaaggttggc tacgtcggcg ctgtggcaca gtggtcgctc ttcctcaagg
    14881 caatcgcctg taactggctc gtcaacctgg ctatcctgct cggtatatgc gccgacgacc
    14941 tgatcggcaa gttcttcgga atctggttcc cgatcatggc cttcgtttcc accggattcg
    15001 agcactgtgt cgcaaacatg tacttcatcc ctgcaggtct cctgaccaaa ggctacctga
    15061 gtcccgacca gatcaccacg atcggtcttg ccaagctgga aggcctgaac tgggtgacga
    15121 tgtggaccaa caacctcgtc ggggcaacca tcggcaacat cgtcggcggt atggtcttcg
    15181 ttggtatact ctactgggtc gccttcagga aggaaatcgc ggcactcaag taaagagata
    15241 tcagatgaaa atcggaaaca tcaatgtgaa gatatcggcc ttatctctgg ctgtttttgg
    15301 tatcttcaca attattttac tcggtacggt tattgccacg ggagagacgt cgatccttat
    15361 ctggggagtt ccgatcctcg ccctgctcct gatcatcccg gcagcgctca actacatgag
    15421 ccagcagcag tatgcatcac tggtgccgat gtacgagcag gaggcaaagg acgtcaaaat
    15481 ccgggcgatc aacctgagca tgatcggtga accggtgcgc ataaaaggcg tcgtcgaacg
    15541 gacgcatttc ctgtttctca accggccgca gtacctcgtc gccgaccgga ccggtgagat
    15601 atcggtcaag atgttcacct cgccggcaga aaacgtgcag aaaggcgatg tggtcgaagt
    15661 gctcggaacc gtcgtcaaac gctacatcct gtccggggat gcggtcatca actgcgtctc
    15721 catacgcaaa gtcgagaaaa atcagcaatc ctgacgcccc cccgggcgcc aagcaactca
    15781 gtgaagggac cggcggaagc gggatgtttc ctgccagaac caccttcgtg cgttgccgtt
    15841 aaaatgccgt atcgagcccg tcatctatcg gcttcggccc cggcctcccg ttcaaatcca
    15901 gaatgtactc ctcaaagaga tggatccggg tgctcacggg aaacgggatc ccgatggtgc
    15961 tgatgaccgc ttctcccgcc tcaagcgtct gaatctcggt gtccagtcgc gagagatcct
    16021 gttttgcgct gcttgcgatg atctcgcgat cgccccggtc ggagagcccc atcaccacga
    16081 aggtgttcaa ctgcgcaagg acccgggcat cgatgttctt cggttgctgt gtcacgacgc
    16141 agagccccac gccgaacttc cgcccctcca ttgcggcttc ccggaatacc cgggtgctcc
    16201 cgagccctga ccccagcacc cgctgggcct cctcgatggt gatcagcacc tgttttggct
    16261 cctcctcgcc tccggcatcc tcgccctggt ggctgcgcat gatcatgcgc gttatgatcg
    16321 agaggacgaa gagttcgctc tgctcgctca tccccgggat atcgatcagg acgaccttgt
    16381 tctggtggag cgcttttaag atatcgggga tcgaggagcc cttcggccgg aggaaacccc
    16441 ggttccggtt catcatgccc gcgatgcgcc gctgcatcac agagagcgtg ctcggcgaga
    16501 agttttttaa gtcccgggca atccgtgggt gccggccgtt gtaggtcgcg gcatcgaagg
    16561 ttgcgaagtc ggtgttctgg aagaagtcga tcacgtctga tccgggcgtg ctctcgagca
    16621 tctccacgac ctcgcgctgg ggcggggagt gctcgtagag caggaggagg tcgggcgccc
    16681 tgaagtcgtt gtagtccagg tgaagctggt ccaggcggta cttattcctg aactgctcct
    16741 gccgggtgga gaagacggag agcccgtcgc ggccagcctg gtagtgcagg agcccctggg
    16801 tgtccttgcc gatcgaggac cgccctcccg ccacgtactc gccgtgcgga tcaacgatga
    16861 ggagcccgaa ctcccgggcc tgcatgcagg aggcgcagaa gaccttcatg aagttgctct
    16921 tccccatgcc cgtcgtggca aagaccccca tatgctgccg catcaccgcg gcgtgaagcg
    16981 ccaccttcac gtccttcaag acgccctgcc cggtcttcat cacgcccacc tcgatctcgc
    17041 ccatcacctg ccgcaggaac gcaaagtcat gggactttgg ccgctccaca cgggagaact
    17101 ttgcagggat cgtccggggc ttccggaagg cgccgtcttc ggtcacgtac ccgagcggca
    17161 tcgcctccac gaggatgaag acgtcctccc ccatcccgta gaagtgctcg gtatgggggc
    17221 gggtatccca gttcgggtcg gagaagttgc agtcatggag gaggtcgctc accttggcaa
    17281 agaacgtcag ccctttgatc cgatcggtga tctttaagat gtccccgagg aagagttctt
    17341 cgtcatgggg gacggcgaac cggtagctga gcacccggtc cccgataagg cgatacttcg
    17401 aggcgtcgtc gccgtcaata tcgatcaaac tcgtcatggt aatctccaaa gatgccggtg
    17461 tagtcggcaa ggcgcatacc gagcaggtcc atgccccgga tgatgtcctg gcggacctgg
    17521 tcgaccgcat ccctccctat cctggtggtg aggtgggcgt cgaggagcgg gtagggatag
    17581 cccgtgaccc tcccgtcgtc ggcgtaggca gcgagtgcag agaagacctg ttcgaccagc
    17641 tcctggctgt agtatttcgg gatctccacc ttgaacgccc gctgtgcccg cggatgaagc
    17701 cgcgcgacat actgctcgcc ccgctgcttc cagaggtatg tctccctgcg gtcgatcagg
    17761 tcttcggctg cggagacgca gaggtaccag ggctcaggga tcccgaggtc ccgggcaagt
    17821 ccctccgccg ccgggacgac ggggtgcccc ccgccccagg tgagcgacgt ccgtttcgtc
    17881 accgcagcga tgagcacgcc cctgaggttg caaaggttta agaggttctc gacgacctcg
    17941 tcgtggcttg catggcggac ctggagcgtg ccgtcgagga cgatgaggtc cccggtatcg
    18001 agttccgcgg ccatctccat cgccgcccag aactcgaggg tatcccgtat gacggcggtg
    18061 ttcctgaccg ggtcgtcgtg gtcgaggtgc gccttggggg ggcagtggaa gcagtcggag
    18121 aagagcgtgt cgaagtcctc gttcccgacc ccggggttga cggtgacgat ctgcaggggc
    18181 gtcgtccggt ggcagagacg cgtgccgccg gcgtacgcgc tgaccgatgc ccggacggcg
    18241 gcaatggcga aactgcccgc atcgagaacg atggtgttgc tcccgtccac cgcgcagacg
    18301 gcgccgtcga actccggccg gcagcggcga tacgatgccg cattaaaccc gctgcacgcc
    18361 gagaaccgcc cggcaaggtc ctccggggcg gcctcccgga tcctccccgt gacccgccgg
    18421 atcgcgtccc ggtagaggtc cttctggtcc atcactcgaa ctcccggact ttactcgcct
    18481 ggtcagcccc catctcgacc atgagcgtcg tcgagaactc gtcctggacc tcggtgatat
    18541 gggatatgag gaggatctgg gggaaatgag cctcctgggt ccgaagggcc cgcagaagat
    18601 tgttccgccg accttcatcc tggctcccga agatctcatc aaagatcagg aacgtggagt
    18661 cgtgcacctc gttcacctcg gcaaggaacc gggagagggc taccctgaga gcgatggcga
    18721 tgtcgtcctg ttctccgccg ctgaaccggt ctgccgggta gtcgtctccc atatcgtgga
    18781 cgaggacggt gaagtcatcg tccagcatca cggtgccgta ccgcccgtcg gtgatctcgg
    18841 caagcactct ccccgcctcc tcctcgatcc tgtccctgac cacctgcagg aggtaatccg
    18901 tgtaatcctt gatgagcgag cgggtcagtt taaggcgccc gacgtcctcg aggagcgcct
    18961 cctgctgccg gaggaggccc tccgcccggg cgagtttccc ggcttcatcc tcgatatccg
    19021 cctgcaggct ggttatacgg gcggcgacga cgtacctctg ctcccgtgcc gcggcaaggt
    19081 ccctctcgca accggcgagc gcctcctgtg ctgccgcgac ctgctgctcg tcaaagccga
    19141 tatctccgat cgccgccctg acccgcagca gttcagcctc cctgttcgcg aggatgctct
    19201 gcttcgcctt cagcgcctgg aggagccgtg ggacttcttc gaggcggatg cgaagttcaa
    19261 gcgctttctg gtgcgctgct tcggcacggc tgcactcctc gccgaggcat gcgaaccgct
    19321 ccggatcgaa cgagaggcgg gcgagttcac tcctggcaat ctctcccctc tcccggagtc
    19381 cgtcaatcct ggcgacgacg gctctccgct cctcttccag tgccggtcgc cgcaggagtc
    19441 gggcctgtgc ctcagcgtat gcgcggtatg acgcctcaag cacctgtgcc tccgcttcca
    19501 ggcgctgccg gatctccggg tcgaacccga gcctggcgat ctttgcctcg gtctcctctt
    19561 tccgggcgag atagccctcg acatcgtcga tgagccgctg cctctcctcc tggagggccg
    19621 ggaggcggct gcactcgccc cggagggtat cggccgtcgc ccgcttctcc gcgagagagc
    19681 ggatccgctc cttgagggcg gcgtgcgccg ccgggtcata cccttccacg ccgagcgccc
    19741 gtatcgcggc acggtgctcc ccggcctcgg cctgccaccg ctgcacgaca cccgtgctct
    19801 gctcgtgccg cgatgagtaa agcgcatgct gctcttttaa gcgctggaag gcgatgcgct
    19861 gccggtggag atcctgcaac ccggcggtga gggcctggtg gtcggcggtc gccctcgtat
    19921 attcgtgctc gaggcctgca agtgtagcct gcatcgacgc gctggtggct gcaaggtcgc
    19981 cgaccagttc ctcgtaatgt tcgccgaggc cctggtggca tgtcggacag gacccttccg
    20041 gacccgcggt gaggatctcc gcccggtgtt cggccagcct tcggatctct tcacggatcg
    20101 tctcctggcg ttcggcacag gcgctgatcc gtgaggtcag gtactccttc cggctctccg
    20161 ccgactcgat ctgctcgtca atggagccca tcccggcgag ctgccgggca aggccggcga
    20221 tctcgctatc gagcaggctc atctcttcgg agccggcatc aacctcccgc aggcaccgct
    20281 cttcctgccg ggcgtaatcc gcagccagaa ggagcacctc ttcacgggcc cggaggctat
    20341 cgagctcccc ggcctccgct tcgagcgtca agaggtgtcg tgccttctcc tcgaccccct
    20401 cgatctcaag ccggttctcc cggacgcgcc gctctgtctg ggaaaagcgc tcgtccatgc
    20461 gggcgagttc ttcacggagg gcgtcgtact ccggttggag cgccttcatc gccgtgatgc
    20521 ggtcgcggag agcctgccac tccccgacct gccgggcaag gccggcaacg gtcaactcgt
    20581 cctctgcaag tgcggcgagt tcggcttcaa ccttcgcgcg gcgttcgccg tactcccgga
    20641 tctgctcgcc cacggcgttt gcttcgagac ccaggcggtc gacaagagcc ttctcctccg
    20701 ccatcgcggc aatctctgct ttcaggggct cgtagcggtc ggcgaggaga gcaaggtctt
    20761 cgagttcgtc ccgcagccgc tgccgctcta cgatctctcc ctccccctcc cggcactcgg
    20821 tacgaagccg cgcgatctca cccgcgagcg cctcttcttc ccgtgcgagg tgcaggtgcc
    20881 ggtcgcgcac agagagcagc cggtcgcgct ccccccgggc gtgctcgagc aacccggcgg
    20941 cacccttctc ggtggcgccg gccttctcgg aatcttcttc tgcaccggca agcccggcgc
    21001 ggagcaccgt gaggcggtcg cgaaccccgt cggcatccag ttcctggagt cggccggaga
    21061 gttcccggaa gaccccttcc cgggcatcga tgaccgactt gaggtcttcc atactgtcct
    21121 ttttgaggta atcgatgccc agcacctgca tgaaccagtc cttccgggac ccggcgcggg
    21181 tctcgatcag ggcgaggagc tccttctgcc cggcgtagat ggtgttccgg aagtcgcccg
    21241 gggccatgcc gacgatccgc tgcacttcct ggcccacctt ctgcacgctg cttgcgagga
    21301 gtttctggtt caggtagagg tttgcttcgt ggagggtcga tgaggagcgg cgcctgaact
    21361 tgcggacgac ggcgtactcg ttgccgccga ccaggaagtc caggcgaacc tcacagacgt
    21421 cctgtgggcc cgcaaaggag ctgacgatgt agttcccgtc aagcccggtc ccctggagac
    21481 cgtagagggc gaagaggatg gcggcgacga tgctgctctt gccggtcccg ttgttcccga
    21541 cgatacccgt gatgccgtcc tggaaggcga tctcctggtc gcggaaccgc ttgaagttcc
    21601 gcatccggag tttattcagc agcactctct ccctctttat ggcgtgcgat gaccgaacgg
    21661 aggaccgcag tcccggtctt cttcacgtac tcctcctccc cgggggcaag gtgctccctc
    21721 tccacgaagc gctcgaactc actgatgtag tccagaccga cgagcgagtc ctcataaaag
    21781 atccgggacg gatcgtcggt ggctatcacc tgcagtttga ggtccagcat ccggttccgg
    21841 atctccacaa gagccttctg gtcgagtgag cggagcgtct cacggcgtat tccatcgagc
    21901 gtgacctggc agatcgcctg gtaggtcttc ttcccggttg catcggccgc atcgaggatg
    21961 ccgtccacga cctcccgggc cgaaaggccg tcgcagttta tccggccgag gccgatcatg
    22021 gaggtgtgcg ggaggtcgat atgcccgaca tcccccgtct ccaggtccac ggcgaggccg
    22081 cccttggtct cggtgatctc cccgtagttg cagtattcaa gcgacccgct gtaccaggcg
    22141 ttgtcggcga cctgcacctg gccgtggaag tggccgagcg cgatgtagtc gaaccggtcg
    22201 gagaggatcg tcgcatccag ttcgtgctcg gcaacggtgt ggagccttct atccttcagg
    22261 atgcttgcaa ggccgtgggt gacgagcacg tttgtgccgg aggagaactc gaccccttcg
    22321 aatgccgtcc ggtatccctc agagaggagc atattcggta tgaggtgaaa gacggcatcc
    22381 ccgagttcga cccgccggta gcggttattg tgagcgacgt agaggtcgct tgcgcgggag
    22441 cctccccgct cgagcacctc gaacggcgat gccgtatacc gggtcttggc catgctgtgg
    22501 ttgcccgcga ccatgagcac cgggatgccc gcatcctgca ggcgggcgag ggcgtcgagc
    22561 gccgtcgtgt aggcgcgggt cttcggcttg acctggtgga agaggtcccc tgcatgaacg
    22621 agcgcatcag gccgtaaggc aatgatccgg tcgatggcgg caaggaagtt gtcgtagatg
    22681 agctgttctc gcaggttcat cccggtctcc ggatctaccc ggttaaaagc cgataacccg
    22741 agatgtgtgt ctgctaaatg aacgatcttc atgcccaatt aatctccatg tcccttgagt
    22801 ataaatgcct gatctgtgcg gtgcgaatgt gtccggcgcc ggaccggtgt ctggaacacg
    22861 cttaaactaa tatatcctca ctgatatttg tatggtgatc acgtgtcaaa aagagccgtt
    22921 gatgcagttt ttcaggcgct cttcatcctc tccgacctcc ggttcctgct ccgggagacc
    22981 gctccgcagc atgaccttga tgatgcacag agggagcgcg ctaaaacctc ccttgagaag
    23041 gtgaagcggc agatcgcaat catcgaagag gagctggtac ggtgaagtgc gcagtcgata
    23101 tcgaatcccg cgacgtcgag gagatgtaca tcaacatcga ccccatccag gcgggcgggc
    23161 ggctcaccgc cgatgccatg aaagcggcaa tatccttcgg tgacggctac tcggtctgcg
    23221 acaactgccg gagccccttc cggctcgact atatccagaa acctcccatc gcccagttcc
    23281 acgcggatct cgccgggtgg ctgaatatgg acaccgcacg ggtggtcccg ggagcgcgcc
    23341 ggggattcca ggcggtcgcg agcacgtacg tggagaaagg cgacccggtc atcgtcacgg
    23401 cgcttgccca ctacaccgag ttcatggcgg tcgaggaggc cgggggaatt cctctggaga
    23461 tcccgaagga cacagcgaac cacatcacgc ccgacgccgc cgcggagaag atcgaggcgg
    23521 tgatccggga gttctcgaaa acaccgcccc tgctcttcat cgaccacgtc gactaccagt
    23581 tcgggaacgt ccacgacgtc gcgggcatcg caaaaatcgc ccaccagtat gacatcccgg
    23641 tcctcgtcaa cggtgcctac tcggtcggga tcatgcctgt cgacggcaaa gcgctcggtg
    23701 ccgacttcgt ggtcgggtcg ggccacaaga gcatggcagc cccggcgccc tcgggcctcc
    23761 tcgcgacgac ggaggagcac gcagggcggg tcttcaggac gacgcaggtg aaaggggacg
    23821 tgaccggccg gacgttcggg ataaaggagg tggagatgat gggctgcacc ctcatgggtg
    23881 tcaccgtcgt cggcatgatg gcctcgttcc cccacgtcag ggagcgggtg cagcactggg
    23941 aggccgaggt ggcgcattcg caggcggtcg tggatgccct cctctccatc gaggggacga
    24001 aggtgctctc cgactccccg agaaagcaca cgctgacccg gattgatacc cgcgagtcct
    24061 tcgatgcggt ggcacagcag cacaagaagc gcggtttctt cctctcaagc gacttaaaga
    24121 agcgcgggat caccggcgtc atcccgggct ccaccaaggt ctggaagttc aacacctt
//
LOCUS       DAJB01000100           26633 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_14972, whole genome shotgun sequence.
ACCESSION   DAJB01000100 DAJB01000000
VERSION     DAJB01000100.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 26633)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26633)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26633
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_14972"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    3767..3778
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5878 a         7984 c         7991 g         4768 t
ORIGIN      
        1 gtgtacgggg cgtgatgcgg gcaattcctc ccccggttga aaccgggggc ctccttgccc
       61 gtcgtactta tgaaaggtgc ccccgaagac gtccgggccg tggttgcgga cggcatcgcg
      121 gacgaacgtg acgaaggaga actcgccgga caggtcacgg taccggagct ggaatgagga
      181 agaggcgcat ccgtcctccg ctacccggca tcagcagatc cgatccgccg agcgcttccc
      241 gcgcagcccc gatcgctcac gggcgggtct atatctcggg cgccggtcaa agcattcttc
      301 acgacccggt cccttacctt cccgggaagg tggttcgcat ggcaggagaa cccaaaatct
      361 ctgatagctt cagggcgctg acctggagag atctggatga atgggcaggc gcaaagacgc
      421 tctcccgcgg taaacgctac cagcgtgaag ggcgggtgcg ggatcttgca tgccttcccc
      481 ggggagacct gatcgcatgg gtggccggta ccgggagata tgcaaccacc gtctccacgg
      541 acggtgaatc catcgaatcc cggtgcacct gccccgtggg agattcgtgc aagcatgccg
      601 tcgccgtcgt gctggaatac ctcgaatcgg tgaagaaagg ccttcccatc ccggccgcgg
      661 ccgaaaacga cccaagactg cggctgctcg attcggattc catggagaat actgccccat
      721 caccggaacc atctctgcgc ccctatctcg aacggctgac aaacgaagaa ctgatcgatc
      781 tcctcgaaac cctggcccgg cacgatcctc acgtacgaaa agcgatcgac gaccggcgtt
      841 ccattgcagc gacaggtgcc ggcccgctcg tcgaagcgct gctctccgag atcgatgccg
      901 ccgccgatga aaggtcgtgg ggcgattacc ggggcggcgg cattcccgac tactcccaca
      961 tgcaggagag gatggagacc ctgctctcga tggggtatgc cgacgaggtt gttctggccg
     1021 gagaagtcct cctgcaaaag gggctcaggc aggtcgagat ggtcgatgat gaaggcgaga
     1081 ccatcgatca gatcgcgtcg tgcatggatg tggtcttctc cgccctcgcc gtctcgtcgc
     1141 ggcctgccca cgagaaaata ctctacgcga tcgaggccga tcttgaagac gagtatgatc
     1201 tctgcgcggg agcggggaga ctgctcgcag gagacttccc ggaagaggag tggggcatcg
     1261 ctgcagaccg gcttctgaaa aggctggaag agatcgcccc ggtacagaaa accgacagtt
     1321 tttcctcaag atatcggcga aatcgcctcg tagccatgac cgtgacgacg cttgataagg
     1381 cgggtcggca agacgaggca acaatccttc agattgggga ggccgacagg acggatacct
     1441 atccagggct tgttgcacgc ctgctccacg aggggcggcg tgatgaggcg ctgcagtgga
     1501 tctatcgggg catctcggag acggagggga cgactcccgg tattgccgac gaactccgga
     1561 ccatcctgcg cgagatgtgg gagagagagg acaactggcc tgccgtagcc gccatccgtg
     1621 ctgaagcgtt cttccgcgag ccgacgtacc cggccttcag gtgcctggag gaggcgtcac
     1681 gccgtgccgg ggtctgggac ggggtcaggg atgctgtgat gcactacctg atcgccgggg
     1741 aaaggcctcc ggcaggagac ggcgcaatcc ccggggtgct ccccgatacg ggcatagggg
     1801 ttcgggcgtc gcgatgggag atcactgctc cggtcgccga cacgcttatc gagatcgcga
     1861 ttgcagagaa gaaaccgggc gaagtccttc gctggtacga ccggtgggaa gaggacaaga
     1921 ttgaccgcta cctgaggcat aatctggagg accgggtcgc ggatgcggtc atcgatgcgt
     1981 atccggagcg ggcgatcgcc atctggaaga agaaggcgga gaggctcatc gccgaaaccc
     2041 gcctgaagtc gtacgaagag tccctgcagt acctgagaac gctgcggtcg catatggaga
     2101 ccccggagtg ggaggagtat cgcggggaac tccggcgtaa gcacgcgaga aaaaggcggt
     2161 tccttgaggt gctcgatcac gtggaggacc gacggatcat tgaggatacc tgattgcgcc
     2221 atacgttgcg gccgcaagtg cggtggtacc tgttggttcc gtagcgacga tgccggaggc
     2281 tgcaacggat gaagaagcgg gatgaggatt ccgagcagtg acgggggccg tcgcggtgca
     2341 cgcggcttcg ctcttctcga tgtcgtagac gaggggtccc tcctttcgaa gccggtttta
     2401 ccccctgcac cgccggtggc gaagtccgga catgaccgcc ttctccgtaa gggggctgga
     2461 cggcacgctc cggtaaaacg gggtggcagt cgccgtcccg gcccgactca cgcgatcagc
     2521 ttctcagcca cgacccgtac tacggggaga ttccgcaact ccccggcgtc tgggcaacgg
     2581 gaagaacgct cgaagagtgc aggagaaacc ttgccggcgt catcgacgaa tggctgatcg
     2641 tacggctccg gcgcgggctt ccgatccctt cggttgccga ccgcaccgta ggagagggca
     2701 cctgagtggg tgccggaaca taggatcgga cctctcctcc gggacagact acttcaggca
     2761 tctagcagag tcccaaacgc tccttcaccc taacgtgcat cccgcacccg gcgatgccag
     2821 catatcccgg gccacttaca ccagaccgtc cggccccctc accctcccct cgtccaccag
     2881 atccgtatag tcgtcgaacc agtaagcctc cgggttgctc ttatagacca taaaccgccc
     2941 gatatcctcc ggattggccg cctggttgta cttgaagtac gtgtggttgt cggtcttccc
     3001 gagaacctca atcttccctg tcgcatggga gatcacgaac ctggcctgtt tcgcaagtcc
     3061 cgaacacctc gaccgtgcct gctcgaagat ccggtacgac tcctccaccg gcactgcaaa
     3121 cgtccggttg ccgatcgtag gacggcactg gaatacgtag tagggattga caccgatgaa
     3181 cgagagtttc tcaaagagcc cggcaagcac ctccgggtcg tcgttgatgc cgcggataag
     3241 gggcgtctgg ttcatgatca ccgctccggc atctttcagg agagcaaccg cccgacaggc
     3301 tgcatcggtc agttccctcg ggtgggtgaa ctgcgccatg atgtaaatgc gcttctcatc
     3361 caggctgtag tcgcggatca tatccagcag cgcgggatcg ttgatgatcc ggaagggatt
     3421 gtatgccggc atcttcgtgc cgatccggat gatctcgacg tgatcgatct cccggagctg
     3481 ccggatgatg tcaaccagcc tgtcggtccc gaggatcagg ggatcgcctc cggtgagcag
     3541 gacgttgctg atctccggat gatcccggat gtaggcaagc cccccggaga tgtccttgtt
     3601 cacctcgcgc gcatcctcga tgaagagacg cttgcggaaa cagtagcggc agaggccgcc
     3661 gcagaggtcg ctgaccagca ggagagcggt ctcccggtac ttgtgctgca accccggcgc
     3721 ccgggtgtac cggtgttccg acgagggatc aaggtgtccc cagggcnnnn nnnnnnnnat
     3781 cagcgagagg tagtagtcgt tcgcacggaa cgcgaagatc tccgtcacct ccgagaggcg
     3841 ggcacactcc tctgcgctga taccggggac ggtatcgagc gatgacagat agatcgggtt
     3901 tgcggtatca tttatggagc tcacggtatc gtcgatcgga ttctggatat catatgtctt
     3961 gttcatggga gtacctccag accgacactc tccagtgcgg gatacgaacc gcaacagtcg
     4021 gggacaagcc gcatgagagc agagatcgtg aatcgaatca gatttacaca aagtgttcat
     4081 gagagtatca tgttctcatg caggaaaccg tcgctccgac atgcgtcatt gagtgtcgga
     4141 ttgcgttatg cagttgaaaa agagtcctca ggatatttat atcttcgctt ggggatacaa
     4201 cgctccagga aaaacgctcg tcccccaact aactacagga gatgccgcat cactttagag
     4261 gagatgctgc atcacttctt cttcctggcc atcgagagga tgtccacgac gtattttcct
     4321 tctttttcca gtccgacgac gacctcgtcc gaccgggcca gtttgtcgat attcaactcg
     4381 tacgaaccgg gaccaagaat gcggatgctc tcaatccgtt caaagacttc gatctcctct
     4441 tttcgccgat cccgccggac ctcaagcacg tcctcgcctg tatcccgggc gatctcatcg
     4501 acggtcttct ccttcagcac gtcatcgtgc cgttcggcct cacggatgta gagaaacttc
     4561 ttcccgccgc agctcggaca tcccttcaat atcttcgtcg agccgtcatc gaactccctg
     4621 ccgcattgtg tacacttgtg aggcatatat ccaccgtgct acccgttatc tccagaatta
     4681 cctgttcatc gcgaggagga gacccatgca ctgatgagat ccttctcctt cttgagcgtc
     4741 ttgagctggt ttgcaggccc gatcaccgtc agccgggtct cggcgcgctt ccccccgaaa
     4801 agcttcccga aaagaccggt cggggcatct ctgacgggat aggtctccat ctcgatcccg
     4861 gaaaacccgt ccggcgcgat ctcccgcatc gtgatctcga tgagtttgct ctgttcctca
     4921 ggcgcgagac ccttctcaag aacaacgatg ttcccttcca tgacatcgtc gaggatcagg
     4981 cggactttct ccattaccgt gagccgatca agacgttcgg ccgaaatgag gtcaatctgt
     5041 acaccctgta tcatctccat cacccgaaat acgacgtcat gttctcgtac aactcatcca
     5101 cgttggttcc ttcaaggctc gagatggaga tcacagggtg ctggggaaat gcactcttga
     5161 tccgcgcggc tgaagcatca gggagatcgt tcttgttcgc gacgatcagg acagggagtt
     5221 tccggctctc gatgatcccg ataagcatga tgttcacctg ctggaacgga tccagtgtgg
     5281 agtcgagcat gtaaatgacg ccatcgatat cctcacggag ccagtgcatc gcctcagcca
     5341 cgccctcggt cgcctcccgt gcccgcttga tcgcctcctc cttctcgatg ccgtactcga
     5401 cgaactcgtt atagtcgatc ttggttgtca cgcccggcgt atcgacgata tcgatggtga
     5461 tcgaactgcc gttctgcccc gtgatggtga tatcttcctt gcgtcgcaca cggcgggtct
     5521 cgtgcggcac ttcactgacc ggcccgaccg catcaccaac ccaatcccgc acaatccggt
     5581 tcgcaagcgt cgtcttgccg gcattgggag gcccataaat cccgatgcgg caggtccgct
     5641 tcttaaagag cgccttcaag aaccccgaga tccttttttt cgtacgaact aagaacgtca
     5701 ttaagctccc tctggtaggc cgatttgcct atacgtaagt gtgttatggc acacacgaat
     5761 aaatataatt atatgttccg tccggcgcgt tttccttatt ttggagacaa accgcaatcc
     5821 cggagcgtga gaattctttt atactgttga gacctagata cacttgcacc tggagaatct
     5881 ggccgtatat cggctcgatt aggagaatcc catgatgaat aatagcgata ccatccgctt
     5941 cgagacacgc ataccactca gggaggtcat gcagagccat ccaacgacca ttgacggggg
     6001 ggagacagtc gcccgggcgg cacagaccat gtgccgcgat ggggtcggca gttgtattgt
     6061 gctgcagaat aacctgccca ctggaatcgt cacggaggag gatatcaact gcaaagttgt
     6121 ggcaaaagac tcaaagccga gcagcatcta tgtcagcgag atcatgagca ccccgctgat
     6181 tacgatcggt gccgacaacc tggtagagga ggccgcagcg atgatggtga agcaccgtgt
     6241 ccgcaggctt cctatcgttg aggagcagca ggtcatcggg attgtgacgg tcagggatat
     6301 cctcaccgtc gcggctgaag tgaacgagct gctggcggac ctcatcgaga tcaaccgcga
     6361 agaagagtac gctatggggg tctgcgaccg gtgcgggaat atgtccgatg atctctcgcg
     6421 ggtcgacaac cttatgctct gccccgtctg tcgggaggag gagcagttgc tatgaaggtg
     6481 gcctcaaacc tgatggtgga gattcccacc ctgcgctacg acgaatacgt cacgaaagcg
     6541 cggagcgtcc tgcgggacga cgtctttcgt gaactctaca tcgtggacga aaaaaatcgt
     6601 ctgatgggct atctcgacat atccgacgtc ctgcgggtta tggacaccaa atcaaacgtc
     6661 acggtcaaag gttttatccg ggaggccgcc caggtcgccc cgaatacgcc cctcgcagag
     6721 gtcggcctcg ctatactgaa tgcccgtaca aacagcgctg cagtcgtcaa tgaagacggc
     6781 accttcaagg gaggggtgct cttctccgaa ctcttccccg tcctgatctc ccgtcgtgac
     6841 atccccggca gggtcgagac ggtcatgacg cgggaaccca tcacctgcgc accggacgac
     6901 cccatactcc gcatctatag cctgatcgtt tcgagcggct tttccgcgtt tcccgtggtg
     6961 cagaagaaag agaccatcgg cgtcatctcc cgccgggatc tcctgcgtgc cggaaatgtc
     7021 cgcacatcgg tgaagaacca ggccgacacc accgtggacc gggtcatgac gacacccgta
     7081 ataacggtga cgcccgatga ctcgatagca acggcaagcc ggctgatgat cgaccacgac
     7141 atcagcatcc tccctgtcgt cgacgagaga aagaggctcg tcggcgttat cgaccgtcat
     7201 gacgccctca gtggccgaat gccgtgagaa tatctcctga cactataatc ccagaggagt
     7261 gagacgatgc aaccgaaccc caataacccg gataagaaac cggctgacag gctcctgaag
     7321 atgccgggca gacgcgatcg cggaccggtc gacttcaaga caaggatcgc cgagtatgaa
     7381 ggcgagatta tggcgatcgc tacccgggac gtcgtcacgg cacagcagac caccacgatc
     7441 atccaggcag tcgagattat gacccgcgaa gggttccgca ggctgccgct cgtcgatgcg
     7501 gggacgcacc gcctccgggg gatcgtgacc gtcagcgaca tcatcgactt catgggtggc
     7561 ggcgacaaat tcaacctcgt acaggtgaaa cacggaggga actttcttgc ggccatcaac
     7621 gagggactcc gcgagatcat gaccccgcac ctggtcacca tgcctgttgc ggggagcatc
     7681 gccgatgcgg tcgatatcat catcaacaaa aacatcggcg ggatccccat caccgacgct
     7741 gaaggagaac tcaagggtat cgtgaccgag cgtgacgtga tgaaggtgct caccaccgcg
     7801 cactcgggcc gcagggcgga agatgtcatg aagtcatcgg tacgtgtcac cagtcccgat
     7861 acgccgatcg gcaaagtctg tgaggagatg gtaaagtgca ggttccggcg gcttccggtc
     7921 gttgtcgacg atcttctctg cggcatcgtc accgccaccg acatcatgag ttatcttgga
     7981 aaagggaaag ccttcgagca gctgacgacg ggagactccg ccgaggttat gggcgcgccg
     8041 gtacggtcgc tcctctcggg ggaacttcac accaccacgc cggaccggaa tatccacgac
     8101 atcgccatcg agatgatcca gcggcgtgtc ggggcgttgc cggtcataga ggattcgcac
     8161 cttgtcggac tcgtgacaga atacgacctt gtgaaggcat ttgcagagag gtgagaagag
     8221 aatgcgtgcc attgatgtga tgtccacgcc ggtgtacgtc gttgcaccgg ccgataatgt
     8281 cgcctatgcg cgaaacctta tgctcaagca ccgcgtctcg agacttcccg tcatggaagg
     8341 ggacgatctt cggggcatcc tgaccaagaa ggatatcgcc taccggctca ggcagacgga
     8401 accgatgtgg cggcggcgcc cgatcgatcg aatcccggtc agtatcctga tggcaccgga
     8461 cccgatcacc gtaaagccgg agaccagcat tcgtgacatc gcagccataa tgctcgatcg
     8521 agatatatcc ggactgcccg tggccgacaa cggcaaggtg accggcatcg tcaccaaact
     8581 ggatgttctc cgttcagccc atgtacgtgg gctctctctc caggtcggcg agatcatgga
     8641 ggatgcggcc acggtcaacc ggtaccactc gctggaccat gttatcgaca cgataaaagg
     8701 aaaaaacgat aaacttatcg tcgttaacga caatggaagc cttgccggca tcattacgga
     8761 aagtaacctc gcattttatg agtatcagga cgagcggaca aatctgccca gaaaagatgt
     8821 cacacacctc aggaaagagg agccggcagg gcagaaacgc ttcagatacg tcgtcgaggt
     8881 atcggcagtt gcagaagaca tcatgagccg cccggtcatc accattccct ccgatgcatc
     8941 cctccaggat gctatcggac tgatgctcga aaaccaggtc aacagtatcg tggtcctcga
     9001 gggcgatgag gtccggggta tgctcaagag agatgatatc ataaaggaag tggcaaaatg
     9061 agcgacagat ttcagttact cgtcaaagac gtgatggcaa aaccaatcac tatcgcgaaa
     9121 tccgcctttg tcagcgaagc gctcgagaag atgctcgatg aaggcgtcga tccgcttatc
     9181 gtgaccaaca acggcacggt catcggcacg acatcccggg cagcgatcgc cgagacactc
     9241 gggagcagga agacccaggc gctgaaggcc acatccatcc atgtcgcgaa cacagtcgag
     9301 gagaacttca cctccgcata cccggaccag agcatcgatg ttctggtgcc gctgctccag
     9361 cactacaagc tggtcgtggt tctcgatgcc gagcaccgcc tcatcggcca ggtgacggca
     9421 ggagacctcc tgaaggtgct ccggccggcg ggcggcctcc tggaggtcat ggaaccggca
     9481 cacaccatcc aggtcgagga gcgggccgtc cacctccgca ggagaatgct ggacaacaac
     9541 atcaaccggt tcatcgtcga ggagggcgac cgggtcctcg gcatcgtcac ggagaccgat
     9601 gtcgcaaaag cgctctacgc gttcaaggat ctcgtggaag agacccacca ggagtaccgg
     9661 atcaggaacc tcctcgtgcg cgacatcatg agcgcgcccc tgatctcggt ggatgcggat
     9721 accgacgtct cggatgtcat cgacctgatg cttaagaaga gcatcagctc cgtcccgatc
     9781 cagcagaacg gcaggatcgc aggcgttgta accaggaaca cactggtcca ggccctgtga
     9841 gacggggcct ctggtactac tggaccattg catctaaatt ttcacgcaac gtcgcctcac
     9901 gcgaagagcg cgaagccgcg aagagtattg actgctgtcc caatcctccc ttcgcacttc
     9961 gcggcttcgc gtgagatggc aaaccttcca cacaccagga ccaacaaaat aaggtgaaat
    10021 agtccactac ctctttttgc gggcgggccg ggggatcggg gtggggtccc tcaacactta
    10081 tgcatacacg ggggaaacat actgatcaca gatcgcatga tcatcctcca cagtttctgg
    10141 accgacaaac ctgccggagt gtttcacatc tggggagagg accccgccct gccccggaaa
    10201 ggggcggcgc agcggggcag aaaaccaaaa aaacgaccga ctctcccgca cccgtttgcg
    10261 gccgatcaca cggcgctcgc cgcggcgctc gatggggccg gtgaacccgg gacagccgcc
    10321 atcctgctcc cggccgccgg gtcaaccccc cttccttcac cggagtgcga acccggatcc
    10381 ggcatgccgg accgggtcga ctgctccctc tatcgcgtcc ccaccatcag cctgcccggg
    10441 acggtacccg tcgcctttct cgcagacctc cctgcaggaa ccggatacgg agccacgcac
    10501 cggttctggg gggaggcggc acggtttgcc ctcggactgg tagtacggca gtcattcgtg
    10561 cccggtaccc acggttggga agccctgatc cgcggagaag atcgcgatcg tgtggcccgg
    10621 ctcgccggag cgctcccgcc ggcctgcctg ttctgggctg ccggagagga tggagccctg
    10681 cccgatccag cggccctggt tacgtcgttc ctcaatcacg cagtccatgc aatcgtcacc
    10741 ggcgctctca gggaacagcc gctcctgcag agatcacggg gccggccgag aaagaccatt
    10801 ccgctcgacg aacaatgggt ggagatgctc tccggccggc gggacgactt caccggtgag
    10861 acagaggaga acacccggtt tctcagggaa ctggaagact ggctatcgcc aacgattgct
    10921 cccgccccac ttcgcgcctg cttccgcctt gaagagccgg aaggggagag cgagcagtgg
    10981 cgcctctcgt tccacctcca ggctgccgac gaccccggga tcgtcattcc ggccgccgac
    11041 atatggaaga ggcgcggaga ggcaagtacc tcgcttgcac agcggtttga agacccgcag
    11101 gaccggctcc tcgccgatct cggccgcgcc tcccacatct accccgcgct tcgaccgggc
    11161 cttgcatccc ggcggccggc ggcggtgcac ctcgataccg aagaagctta cgcgtttctc
    11221 agagaatggg cttcgcttct catagagaac ggctttggcg tcatcctccc tgcatggtgg
    11281 aaaggtacag cctcccggtc cacccttcaa cttaaggtga agcctgccag ggagggagag
    11341 gggcttggtc tcgccacgct cgtcgcgttc gactggacgg tcgcccttgg agaccggatg
    11401 ctctcaccgg aagagttcga acaactcgcg ttcctgaaga tgcccctggt gcgaatgcac
    11461 ggccggtggc aatcgatcga cagggacgag atcaaaaagg cgctcaatgc attcacgaag
    11521 aagtacgcta ccggcaccat gacgaccggc gaactcctcc gggtcatcgc aggaaccgaa
    11581 ccggacggcc cggcggtgag tgaggtcacg gcagacgggt ggctccgcgg gttcctcgac
    11641 cggtcggccg aaaggaggct gattgagccg gttccgcagc ctgcggcgtt ttcgggcacc
    11701 ctccggccgt atcagcagcg cgggctctcg tggctatcct ttctcacgcg caacgggtgc
    11761 ggcgcctgtc tcgccgatga tatggggctt ggaaagaccg tgcaggtgat cgcctatctc
    11821 ctcgctgaaa acgagcgaaa atgcccggca acaccgacac tccttctctg ccccacatcg
    11881 atcgtcggga actggcagcg cgagttcgcc cgctttgcgc cggatctcac ggtcaccgtc
    11941 caccacggca gcaaccgggc ctgcggcgac gtgtttgccg cacaggctgc cgggtccgac
    12001 gttacgatca ccacctatcc gctggccgta cgcgacaggg cgctcctcgc atccgtggcc
    12061 tggaactgca tcatcctcga cgaagcccag aatatcaaaa atccctccac aaaacaggcg
    12121 caggcagtcc gggcgttcaa agcatcgcgg cggatcgcgc tgaccggcac gccggtggag
    12181 aaccgcctcg ccgaactctg gtcgatcatg caatttttaa accccggcta cctcggaacg
    12241 tttgattcgt tccagtcggg gtttgccgtg ccgatcgaac gttatcacga tcctgcaaaa
    12301 accgctcatc tccgggacct gatccagccg ttcgtgctcc gccgcatgaa gaccgaccgc
    12361 tcgatcatca gcgacctccc ggagaagatg gagatgaagg tgttctgccc cctcactacg
    12421 gaacaggcga cgctctacga ggcggtcgtg caggagatgc tcgaacgtgc cgaaggtgcg
    12481 gagggcatcc agcgcagggg aatcgttctc ggggggctca cccgcttaaa gcagatctgc
    12541 gaccaccccg cgctcttcca gcacgaaaaa acccttacca ggggaaaatc cggaaaaatc
    12601 gcgcgccttg aggagatgct cgaggaggtg ctggacgcag gcgaacgcgc gctcatcttt
    12661 acgcagttcg cagagttcgg aacgcttctt gaagggcacc tccgcgaacg gttcggcacg
    12721 gccgtcatct acctgcacgg caaaacccct caacagaaac gcgacgcgat ggtgcagcgt
    12781 ttccagcagc cgggagggcc gtccctcttc cttctctcgc tgaaggccgg gggcacgggg
    12841 ctgaacctga cggcagccaa ccacgtcttt cacctggacc ggtggtggaa tccggccgtc
    12901 gagaaccagg cgaccgaccg tgctttcagg atcgggcaga cccgcgacgt ccaggtccgc
    12961 ctcatgattg ccgcaggaac ccttgaggaa cggatagaca tgctgctcga agagaaaaag
    13021 gcgcttgccg accgggtcac cggaacgggg gaggactgga tcgggtcgct ctccaccgac
    13081 cagctccggg atctgctgac cctgcgccgc gacgcggggg aggactgaag aatgggctca
    13141 tggtattatc cccggtttcc cccgtccgtc ccgcggccgg tgaccggcgg cctcaaggcg
    13201 cggagtaagc ggggatcgat cggcgagcaa tggtggtcgc aacggtttat ccaggtgctc
    13261 gaagagacgg ggaacgccgc gcggctgcag cgcggaaaga ggtatgcccg gatggggcag
    13321 gtgacgggta tacggatcac gggaggagag gtgcgggcag cggtgcaggg atccgccgcg
    13381 aagccctaca gcgtcaccat ccgcctccgc cccttcaccg acgacacgtg gaaccaggtg
    13441 tttgaccgga tggctgcgca ggcgatcttt gcggcccaac tcctggcagg cgttgtgccg
    13501 cacgagatcg agaaggtctt cgaagaggcg ggagcatctc tcttcccggc ctctggaaag
    13561 gacatccaga cggagtgcac ctgccccgac tgggagaacc cctgcaaaca catcgcagcg
    13621 gtctattacc tcctcgcaga gcggttcgac gaagacccct tcctcatctt cgccctccgg
    13681 gggcgaagca gggatgaggt tacggcagca ctcagggagc ggcggacgag tgagccggat
    13741 ccggcgacgg cggaccgggc caccccctcc gtcgaccgct tctggcaggg ggacgacacc
    13801 cttgccgggt tcggcatcga tattaccggg gatgtctcga ccgaaggagc ggccctgaaa
    13861 acgcttggcg attccccgtt caagatcagg gacaggaacc tctcagcgat ccttgcaccg
    13921 gcctacccgg cggcacggga atatgcacgg aaaattgccc ggatccccga tcggtcggaa
    13981 cctggagaag aaccggcagc agacccgtca ccctgcagag agaaccggca ggactccggg
    14041 cagcgggcgg ggaggtgatc cccgcaacac acggggaaac agccaccccc tccacccggc
    14101 acgagcgaaa cggtcgggtg accctctata gtagtgccaa aacctccatc cgcccacccc
    14161 ccacaccagc accaatcatt aatactccac agccagaaac ggtatcagga tatttccatg
    14221 aaaccaacgc agccagtcaa accgaatgcg gatgtaacgg cactccttct gtccatgagc
    14281 aggaccggct tccagggaag aaaactcggg gagtcgctcg gcgtctggac caggatggtc
    14341 gaagatccgg actgcacgat attcatgggg ctgtcggggg cgatgatccc gggaggtatg
    14401 cagaacatac tcatcgaact cgtcaggcac cgctacatcg atgtcctggt ctcgacgggt
    14461 gcaaacatct tccatgacac ctgcgagcac ctcggtgtcc ggcactacct cggccaccac
    14521 cacgcagacg atgcggcact cttcgagaag gggatagacc gcatctatga cgtcttcgcg
    14581 tatgaagagg agttccggag tatcgacgcg aagatatcgc ggtttgccga cgagatcgca
    14641 ccgttccggg gttcctcgcg ggagttcatc cggctcctcg ggaactggct tcgtgagcac
    14701 aggccggagg gacagtcgct catcgccacc tgtgccgagt acggcgtccc gatcttcatc
    14761 cccgccctcg gcgactcatc catcggcatc ggcctcgtga tggcgcgcag gcgcggggtc
    14821 gacgtcgata tcgaccagct cgccgatacc gacgagatca cgcgtatggt cgaggagtcc
    14881 aagaagaccg gcgtcatcta catcggcggc ggcgtgccga agaacttcat ccagcagacc
    14941 caggtgatcg cctcgatcca cgaacagaac ctcggcgggc atgcctacgc cgtccagtac
    15001 accaccgacg cccctcactg gggcgggctt tcggggtgca cgtttgagga ggcgatcagc
    15061 tggggcaagg aggcagtgga gtccccccgt gtccagtgct tctgcgacgc cacgatcgcg
    15121 ctccccattg ttgcgtcggg acttatcgga agtggcgttc ggcgggctcg ccgctccccc
    15181 tgaaaacacc acaaatatta tcaaggtatc atgccaaaat gaataggcac gagtcataga
    15241 atgctgtgtc tggagcaaca cccgcgtgtt gcgagtatcg aaagatgcga agttctctgt
    15301 tgaggtagcc aagcctggta tggcgcaggt ttgctaaacc tgtgtccccc tgggactcga
    15361 gggttcaaat ccctccctca gcgctttcac cgacaacgtc gatgatgagg cgatcacatg
    15421 gatgaagaag agataaagta ctttgttcga gtcagaaaca ctgatctcga cggcaccaag
    15481 gcagtgcaca tcgcactgac cgggatcaag ggcattggtc cgcacacatc gcgcaccatc
    15541 accgctcttg ccggcgtgga tccacatgcc gtactcggca agctggacga cggatccgtc
    15601 gagcggatcg caaacgccgt tgatacgtac accgaacagg tgcccgactg gatggtaaac
    15661 cgccaaaagg acatctacac cggagaggtt cgtcatctcc tcggacccga tctcacgatg
    15721 atgaacgacg acgatgtcaa ccgtatgcgc aagatgcgca gctaccgggg catcaggcac
    15781 gagaccgggc agaaggtccg cggtcagcgc accaagtcca ccggaagaac cggcacgacc
    15841 gtcggcgtca agagaaagaa agagtaatgc ggtgatcgaa tgggataccc agggaaaaac
    15901 cacaagcaat acgcgacgcc caagcggcgg ttcgagaaga caagacttga ggacgaaaag
    15961 cggctcatca tcgattacgg cctgaggaac aagcgtgaac tctggaaggc ccagagcgtg
    16021 ctgcggaaat accggtctgc cgcccgtgag ctggtggcac tgcggtcggg tggcctcagc
    16081 actgaggact accaggcgaa gaaggaccag ttcctcaacc atctctaccg ctacggcctc
    16141 gtcggtgaga acgccgatat cggcgacgcc ctcggcctga aggtcgagca gcaactcgat
    16201 cgccgcctcc agacgcaggt catccgcaag gggctcgcac gatcccccaa gcaggcccgc
    16261 cagttcatcg tccacggaca catcgcgatc tccggtcgcc gcgtgaccgt accgagctac
    16321 cgtgtcgcga gagcccagga gccggagatc agttactacg gcccctcccc gttgacgaac
    16381 gacgttcacc ccgagagaag ccgcatcgcc cgcacaggaa tgaggtaata tcatggcaga
    16441 agagaaatgg ggcattgcac acatctttgc ctcctttaac aataccatca tcaccgtcac
    16501 cgacctctcc ggagcggaga cggtcaccaa gagcagcggc ggcatggtcg tgaagcagga
    16561 cagaaacgag agctcgccgt atgcagccat gcagatggca atccaggtcg cacagaacgc
    16621 gcgcgacaag ggcatcaccg gcgtccacgt aaaggtccgc gcacccggac ggggcaaaca
    16681 gcggagcccc ggacccggcg ctcaggcagc catccgcgcc cttgcccgtg cagggatgag
    16741 gatcggccgc atcgaagacg tcaccccggt gccccacgac agcatccgcg gtaagggcgg
    16801 ccggagagga aggagagtgt aatggagata gcgttttctc gactggatga gagagtcgcc
    16861 aaatttaccc tgagcggcgt ttccacgtcg ttcgccaata tgtttcggcg ggcgatgatc
    16921 agcgaagtgc cgacgctcgc catcgaagat gtccggatct acgataatac aagcgtcctc
    16981 ttcgatgaga tgctgacgca ccggctgggg ctcatccccc tgcggaccga cctgaaacgc
    17041 tacgcgcggc gtagcgaatg cacctgtgaa ggcgtcgggt gtccggtctg caccgcaaca
    17101 tatacgctct ccgccgaggg gccaaggacg gtctactcaa gcgacctgat accccaggac
    17161 cccgacgctg caccggcaga agagaagatc cccatcatcg acctcgggcg agaccagaag
    17221 atcgtgctcg aggcatacgc catcgtcggc accggaaacg agcatgccaa gtggcaggca
    17281 acaaccgcct gcggctacaa gaactacccc aagatcacca tcggtgagtt gtgcgacgga
    17341 tgcggtatgt gcgtcgacga atgtccgcgc aacgtgctcg aaacagcaca gggtaaggtt
    17401 cgggtcattg cagaacggca ggaattttgt tccctctgca ggctctgcga gcaggcatgt
    17461 gttgccggcg gcatcggcac cgaggcggcc atccgcatcg gcactgacgc gagcagcttc
    17521 atcttcgtgg tggagggcga cggctcaatg cccgtccggg agatcatcga gagagcgcta
    17581 cagtacatcc agaagtcgtc agacgatctg gtagacgtga tgaacgagat aacgggagag
    17641 gggactgaat gaagaagaca accgagaata aatcgaaccc ccggctggtc gctctcattg
    17701 tgacgctgaa agatgcgtca cgcgtacacg aggcgaacat ctggcgcgag attgcaaaga
    17761 ggctggatac accccggaag aactacgcag aggtgaacct cagcaagatc gaccggtacg
    17821 cgaacgaagg cgagacgatc ctggtgccgg gcaaagtgct cggcagcggc gcgctcaacc
    17881 tgccggtaaa gatcgccgca ctggacttct ccgaggccgc gatgagcaag atcaccggtg
    17941 ccaacgggac gtgcatgact atcgaggacc tcgttcggga taacccgaag gggagcaggg
    18001 tacggatcct caggtgagac gaatggttac agttatcgac gcagacggat tactgctcgg
    18061 aagaatggcc agcctcgtcg cgcagcgcgc gctcgccggc gaagagattg ccctggtaaa
    18121 tgtggagaag gcaatcgtct ccggaagccg ggcacatgtg cttgcgaact acacccacaa
    18181 gcgcgagcgg ggatcccgtg agggcggccc gttcttcccg cgcaggcctg accatatcgt
    18241 caagcgcacc atacggggca tgctcccgta caagcgcgag cgcggcatgg ctgcattcaa
    18301 gcggatcaag acctatgtcg gggttcccat ggaactcgtc gggatagaga ccgaaacgct
    18361 cgaaggggct cacatcgacc ggctgagcag cccgaagtat ataacgatcg ggacgataag
    18421 caccaacctc ggagcaaaat attaagagag gtgatggaat gacaaagatc atcaacacaa
    18481 gcggcaagag aaagacggca atcgcccggg cgaccttaaa acccggcaac ggtcgggtcc
    18541 gcatcaactc cgtgcccctg gagatctacg ggaacgagtt gactcgcatg aagatcgccg
    18601 agccgctcct gctggcgccg accgcactcg acggggtcga tgcagcgatc gatgtctccg
    18661 gcggaggcat catgggccag gccgaggcga tccggacggc actcgcgcgc ggcatcgtgg
    18721 agtggcacaa cgacccacgg ataaaggatg cgttcctcgc gtacgaccgg actctgctcg
    18781 taaacgactc acggcagaag gaaaccaaga agccgcacgg cccaggcgca cgcgcacggt
    18841 tccagaagtc ctaccggtga gcaaaagaga tataagggat gcaggaccat tattatgata
    18901 cccgtacgat gttttacatg cggtaaagtc atctctacag cctggaagga gttcgcgaag
    18961 cggcgagatg caggcgagga tccaaaaggg atcctcgacg atctcggcct ggagcgttat
    19021 tgctgcaggc ggatgctgct gacacacaag gtagtagtgg aggacctgaa cccataccaa
    19081 tgaggggtcg tggggtagcc tggccatcct atggcgttcg ggatgctgtg acctgagttc
    19141 aaatctcagc gaccccattt tatcattaat gattttgagg ctacacgatg gaatcgtata
    19201 cccgatatga acgagcgcgg attatagggg ctcgtgctct gcagatatcg atgggtgcac
    19261 ctgttctggt taaaacacca aagacagagc cactcgagat cgcgcttgaa gagtacgatc
    19321 aagacgtgat ccctataacg gtgaagagaa agtagtgatt taatgacgac catcgaacag
    19381 atcatcttaa gaacgatcct tgatagccgc gggaatgaga ccgtcgaggc tgaaatccac
    19441 acagattgcg gctttgggcg ggctgcggca ccgagcggag caagcaccgg gacctatgag
    19501 gccagagtgc ggccaccgcg cgaagctgtc gaggatgcac agaagaacct gattccatcg
    19561 ctcatcggtg aggacactcg cgatcagatc acattcgatg cattgctcag agagcacgac
    19621 gggacacccg acttcagttc aatcggtgca aacgtcgctg tagcactctc gcttgcgtgc
    19681 gcaaaggcgg ctgcatcgtc tctcgacctt gaactcttcc ggtacctggg cggtgcattt
    19741 gccggcgaga cgcccctgcc tctcggcaac atcatcggcg gaggcgcgca tgccccgaac
    19801 gccacatcca tccaggagtt cctggtggtt cccaccgggg cttccggcgc aacagagggc
    19861 gtcttcgtga acgctgccgt ccatggggca gtgaagaaga tcctgcagaa gcagggcaga
    19921 ctctccggta aaggggatga aggtgcctgg gcacccgcaa taaccgacac cgaagcattc
    19981 gagatcatcg gcgaagcaat cgccaccgtc actgatgaga tgaacgtcga ggtccggatg
    20041 ggcatcgacg tggcggcaag cgaactctgg gacggcaagc agtatcgcta caaggatgcc
    20101 gcacggaccc gggaagacca ggtagcctac atggcggatc tggtcgacca ctacaacctc
    20161 atctacatcg aggatcccct cttcgaggaa gacttcgatg cgttcgccga cctgaccgaa
    20221 caggtcgggg accgctgcct cgtctgcgga gacgacctct tcgtcacgaa tgtcgaacgt
    20281 atcacgaagg gcatcgagac gaacgcggca aactgcgtgc tgatcaaacc caaccagatc
    20341 ggaacgctca ccgatacctt cgaggcgata cacctcgcga gggagaacgg catggaaacc
    20401 gtcatgagcc accgttccgg agagaccacc gacgcgacga tcgcgcacct tgcgaccgcg
    20461 ttcgagtgca tcttcctcaa gactggcgtg gtcggcgggg aacggatagc caaactcaac
    20521 gaactgattc gcatagagga gctgatctaa cttgactgga aacgaacttg aaatcgaact
    20581 gaaagaacct ctggtgcccg ttgaagagta cctcgcagca ggcgtgcaca tcggcaccca
    20641 gcagaagagc aaagacatga tgaagttcat ctaccgcgtc cgcggggatg gactgtatat
    20701 cctggacatc caggcaaccg acgagcggat caaggtcgct gcggagttcc tcgcacgcta
    20761 cgaacctgcg aagattctgg tggtcacctc ccggcagtac ggccagtacc cggccaagaa
    20821 gttcgccgat tccatcggcg gcatggcggt catcggccgc ttcatccccg ggatgctcac
    20881 caaccagcgc ctgaacaaat atatcgagcc cgacgtgatc gtggtgaccg accccatcgg
    20941 tgacgcccag gcaatcaccg aggcggtcca ggcaggcatc ccgatcgtcg ctctctgcga
    21001 caccaacaac atgacgaagt acgtcgatgt ggtcatcccc accaacaaca agggcagaaa
    21061 agcgctctcg gtgatctact tccttctgac gaaggaactg ctccgtctgc gcggcgtgac
    21121 cacatcactc actgccgaag actttgagac agagttataa gatccgaagc acaagagctc
    21181 aaccttgatg gatatgcgcc catgcagtgt agcgggaatg ttctatcctg ccgagcccag
    21241 gcatctggag caactgctgg agacgttctt ccggaacagg accccgggcg tcgatgcccg
    21301 gggaatcgtc tctccccacg cgggctacgt ttattcgggg gagaccgcag cctgtgcttt
    21361 ctctaccata ccacctgatt ttgacggcac attcgttgtc atcggtccga gccaccgggg
    21421 atacatgacc tgtgtctccg ccataccgtg ggagacccct ctcggtatcg tcgacgtcga
    21481 tacggagttt gtcgacgcac tggatatcga gatagatgag gtatcgcacc ggagcgagca
    21541 ctcgatcgag gtgcagatgc ccctcatcaa gtaccggttt ccgcgagcga gggtcgcgcc
    21601 cgttctcatg ggagaacaga gttatgaggc agcggcactc ctcgcggatc aggtgctccg
    21661 ggcggcaggc cacacgggaa gaaatgtgcg gatcgtcgcc tcaagcgact tctcccatta
    21721 cgtcccggat caggtagccc gccggcagga cctcttggca atcgacgcac tcaaaaccct
    21781 ggacataccg gaattttatc gccggctcca ggagaccggc gctacggtat gcggctacgg
    21841 cccgatcgcc gccatgtgca tcgcctgccg gtcgctcggt gcagagcgag gggaactctt
    21901 gcggtatacg accagcggcg atgtgacggg ggatctcgat caggttgtgg ggtatgcggc
    21961 gatagcggtg gtgtaattgg caacgtggag cgcgccgggc aagatattcc tgttcggcga
    22021 gcatgcagtg gtctacggga agccgggagt cgcgatggcg atcaagcccc gtgtatttgt
    22081 cacggtgagg aagtcccgga acccgacacg tgcgaagtca ccctacatcg atgaatgttt
    22141 caaactgatg ggtgtccggg gcagtgtcta cgtccactca caactccaga gttcatccgg
    22201 gctcggatcg tcggccgcgg tgacggtggc gacactctcc gccatcaacg acgagttcgg
    22261 tctcgggcgc acccgcgagt acatcgccga tgcggcgttc tccgttgaga agaaggtcca
    22321 gaaggggagg gcgagcccca ccgacaccta cgtctccgca aacggaggga tggtcctcat
    22381 cacagggaac tccaagcgga gactcccccc cgaggggttg caggtcgttg tcggcaatac
    22441 cctggtgccg cacagcaccg cgagaatggt agaacaggtc ggggatctcc agaggaggca
    22501 ccccgacgtc gcaaacccga tactggatgc catcggggcc ttgacgcttg ccgccctcca
    22561 taacatcaac aacccaaagg aactcgggca gtatatggac atgaaccatg ccctcttaga
    22621 agctctcggc gtgggacacc ccacaagcag caagctcgtg cttgcggcga gggcgagcgg
    22681 cgcctacggg gcgaagatca ccggggcagg aggcggcggg tgtatcatcg ccctctgccc
    22741 ccggcgtgca aagagccggg ttgccggggc catcgaggcc tgcgaaggga aagcaatcat
    22801 caccacgata gacacggatg gcgcgagaaa agagaagcat gactgagacc gtggtactga
    22861 aactgggagg gagcgtgatc accgacaagt cgggagaatg cgctatcgat cacgcccgcc
    22921 tgcacgaggt tgcgggccag ctcgccgtac ggagagcgcc cgcactcgtg ctcgtccatg
    22981 gtgcagggtc gtgcgggcac cccgaggccc ggcgttaccg catcaacgac ggtctcaccc
    23041 ccgagaacct gcccggcgtc taccatacac ataccgccgt ctcgcgcctg aacgctgcgg
    23101 tcgtcgatgc cctgcgggat gcgggcgtcg aggcgatcgg catccacccg ttcgatctgg
    23161 ctctcaccga aggcggacgt ctggtctcat tcgagacccg ccatatcgcc gaaatgacag
    23221 aacacggcat tgtgcccgtg ctgcacggcg acgtggtgat ggaccttctc cggggatcct
    23281 gcatcgtatc gggggaccag ctggtgaccc gcctcgccgt tgctctcgcg agccggcgcg
    23341 tgggtctcgc aacagatgtc cccggcgtgc tgcagaacgg atcggtcatc ccgcgcatcg
    23401 accggagtat agcagagtcg tttacgatcg gcggatcggg aaacaccgac gtgaccggag
    23461 gcatgcaggg caagatcaac gagctcctgg cactggccga taccggcatc gattcgcata
    23521 tcttccatgt ctcgaagatc ggccggttcc tggacggcac cgggcatggc ggaacaacaa
    23581 taaccagaag aggcatgcca tgacagaaga gaccgccaca tcctcacgaa aacgggatca
    23641 cctcaacatc tgttgtgaga accccatcga ggccggcgac gccggtttcg gagacgtgcg
    23701 actggtccat aacgctcttc ccgagtgcaa tatggatgcg atcgagaccc ggacaaggtt
    23761 cctcggtaga acactcggct ctcccctctt cattgcagcg atgaccggag gacacccgga
    23821 caccctcgag gtaaaccggc ggctcgcacg tgctgccgaa cggtataacc tcggcatggg
    23881 tgtcggttct cagcgagcgg cactggaaaa gcccgaactg gaagagagtt tcaccgtcgt
    23941 gcgggaggag gcgcctcatg ccttcctctg tgcgaacctt ggaatcgttc agctccgcga
    24001 ccacggcatc gagtgggcag agcgggctgt cgagatgatt gatgcacagg ctatcgccat
    24061 ccacgtcaat tcactccagg aagcaattca gccggagggc gatcataatg cagagggggg
    24121 ccttgaagcg ctccgcagcc tctgcgaaga gttctcctat ccggtcatcg tgaaggagac
    24181 gggttccggc atatcggccg ggactgcaag ggttatccgg ggagcaggag caagcgctat
    24241 cgatatcggc ggttatggag gcacgtcatg ggcaaagatc gaacgcctgc gggcgaacga
    24301 ctccggtctg gccgatctcg gggaagcatt cctctcctgg ggcataccga cggtggtaag
    24361 cctctgcgag gtgcggacga caggaggccc gatcattgcg acgggcggac tgcgttcagg
    24421 aatagatatc gcgaaatcta ttgccctcgg ggcggaactc ggaggcatgg ccctccccct
    24481 cttaaaaccg gcgatggaga gcgaggaagc cctctttgca gctgtcgagg cgatacaccg
    24541 cgaactcgac gtggcgatgt tcctgacggg atcccggtcg atacgggatc tctcccacgc
    24601 acggacgtat ataaccggac tgacccggca aatgattgag accagcgact aaccacaaaa
    24661 acatccttac aaggaggcta catggatatt gagattatag cagtgggcgg atataacgaa
    24721 gtcggcagaa atatgaccgc cgtccgctgc ggaaaagaga ttgtcatctt tgatatgggt
    24781 ctgcgtctgg accaggtgat gatccacgag gatgctgata tcgagaatat gcattccctg
    24841 gacctgatcc agatgaaggc cattcctgat gataccatca tgaatgcggc agaggggagc
    24901 gtcaaggcga tcgtctgttc gcacgggcac ctggaccata ttggtgcaat accgaaactt
    24961 gcgcaccgct acaacgcccc gatcatcagc acgccgtata cctcggaact gatcagacag
    25021 cagattgcag gtgaacagaa gttcggagtg aacaataagc tcttcgtcct gaaagcaggg
    25081 cagcgctaca cgatctcccc gcacctcacg ctcgagttcg tgcaggccca gcactcgatc
    25141 atcgatacgg tctttccggt gctgcacacg ccgcggggag cggtcgtcta cgcgaacgac
    25201 ttcaaactgg accggacgcc ggtgctcggc gagccgccgg actttgcccg gctgcggcag
    25261 atcgggaagg agggcgtgat cgccctcatc acggagagcg tcaacatcgc cgataacggc
    25321 cggtgcccga gcgagaggat cgcgcgggac ctcgtacggg ataccatcac gagttacgag
    25381 gacgacaagg gtgctctgtt cgtctcgacg ttctcgtcgc atatctcccg tgtcaaggcc
    25441 atcgccgaat gcgcccacga gatcggcaga aaaccggtcc tgctcgggcg gtcgatggag
    25501 cgctacagct cggcggccga acagttgaaa ctggtcgcgt tccccgagtc cctctccatg
    25561 ttcggcaacc gccggaccgt tgataggacg ctgcgccgga tcatgaagac cgggaaggac
    25621 aagttcctcc cgatcgtcac cggtcaccag ggagagtcgg gtgccatcct gacgaggatc
    25681 gtgatggggg atacccccta caagatggag aagggcgaca agatcctctt ctccgcaaag
    25741 gtgatcccga acccgatgaa ttacgggcag cgttacctgg tcgaggcccg cgccaagatg
    25801 gcaggcgtgc ggatcttcga cgaactgcac gtctcgggtc acgcctacaa agaagaccac
    25861 tacgagttcc tgcatctctt aaacccccag cacgtcatcc cgtcgcacgg cgacatcggg
    25921 atgaccggcg gctacgcgag gttcgcagag gagatcgggt atacactcgg caacgacctt
    25981 catatcctcc ggaatggggg cagactgttg ataacgtagg agacaatgca tgacgacgct
    26041 tgaagactac ctggagaaga atgctgagcg gatcgacaag gtgatccacc gttacttcgg
    26101 agatgtccac ggcgacctct tccgggcgag cgcccacctg ctgcttgcag ggggcaaacg
    26161 gctccgcccg gcagtcgtca tactcgctgc ggatgcagta aagaaaggaa gttcggacga
    26221 cctgatccct gcagcgctcg cgcttgaact gactcacaat ttcaccctca tccacgacga
    26281 tatcatggac ggcgacgttg tgcggcgcgg cgtcccgacg gtgcacaagg tctggaacga
    26341 accgatggct atccttgcgg gggatgtact ctacgcgaag gcgttcgagt tcatctgcct
    26401 ctctgaggcc gaaaaccccg ccaaaatccg ggcagtgaag atgctcgccc ggacctgcac
    26461 cgagatctgc gaggggcaga gcatggatat ggcgttcggg acgagggacg acgtctcgga
    26521 tctcgattac cttgagatgg tcagcaagaa gaccggcgtg ctctacggcg cgtctgccgc
    26581 catcggcgga atccttgcgg gtgcaaaccc ggtccaggcc gatgccctct acc
//
LOCUS       DAJB01000101           17703 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_31728, whole genome shotgun sequence.
ACCESSION   DAJB01000101 DAJB01000000
VERSION     DAJB01000101.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17703)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17703)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17703
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_31728"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3591 a         5112 c         4886 g         4114 t
ORIGIN      
        1 gggggctcct cctcgagggc acgctgcttg atcgcctggg agagctgcat cacgagtttg
       61 acgttaacgt cggcggagag gagcgcccgc tgcaggtcgc gcaccagttc gtcgaccgcc
      121 acccggtcga tcaccgtctt gccggcaagt ttcttgacgg catccttcaa ggatgcaccg
      181 aggttatcga gcatcaggcc tcatctccta agagttcgtt caccatggca cggggctcaa
      241 acggcatgat gtcgtcgtac ccctggccaa tcccgaggaa gaggattggt tttccaacgg
      301 tgtgcgcaac cgatatggcc gcgccgcccc tgggatccat atccgccttc gtcaggacga
      361 ccgcgtcggt gccgaccgcc ttgttgaact cgtcggcccg gatgaccgca tcgtttcctg
      421 ccaccgcctc gtcgacgtag acgacgaggt cgggcttcat gacccgccgt atcttctcaa
      481 gctggctcat gaggtttgcc cggttatgga accggccggc cgtatcggcg aggacaacat
      541 cgatctcgtg cgctctggcg tactggaccg cgtcgaagag gaccgctgaa gggtcggcgc
      601 ccgcctgatg ctggatcacc ttgataccaa ggcggtcggc gtgggtccgg atctgctcga
      661 tcgcccccgc acggaaggtg tcgccggcac cgatcaccac tgaaaacccg ttcttctgga
      721 ggtagtggcc gacctttgcg accgtcgtcg tcttcccggt cccgttcacg ccggtgaaga
      781 ggatcttcac gggccgctcg tgccccgcga tgtattcacg gaggtcgagg ccttccccca
      841 gcaccccgca gagtgcggac cgcagggttc caacgaccag gtcgtcgacc gaggagccga
      901 tctttctgtg ccggcccacg aggtcccggc gcatccgggc gatgatctcg tccgtgaccg
      961 ggagtgcgac gtcgttctcg aggaggacca tctcaagttc agaaagaggc tcctcgatat
     1021 ccttctcctg gaggaggact tcccgctccc ggacaaggac tttaatcttg ttgaaaaacg
     1081 tgggttcctc ccgctcttcc ccggtgaccg ggggttcggg caggagggcg cgggagggcg
     1141 cgggctctgc gacctccggt acttcggcaa ccagcggtac cggttcggtt ccggcagctt
     1201 cctcgatatt cgaggtaaat ttatccctga tagtctgaag ttttttcttt aaggaatcaa
     1261 acattatcag cttccgccct ggcctgcctg ggcctgtcgg gccccccggt aagctgcctc
     1321 gaggcgccgg gagatctccg ttgcctgccc ctgcaactgc tcgaccgaac ctgcgacttt
     1381 cttccccacc gcctcaagtt cggctatccg atcccggaga tactccaccg catccgcgct
     1441 ggccctctca acagagacgt cggccccgat gttcacgaga acgtgaccgg catcaagcac
     1501 cttcacccgg agaagagcgc cgcccccgac gggaagaagg acgataccgt ccttgttctc
     1561 ctcgatggcg cgaatggatt cgatggcagc ggcagactcg aggcgctgct gctcaatcat
     1621 accgagctgc tgcgtcagga gctctatctg ctgcccgtac tcgttcaagt acatctggag
     1681 ggactgtatc tcgcgaggat ctgcctggtc cacgtcattc accagctaca acattaaccg
     1741 tcttgatagt gatataactt ctcttcaggc ggtgtttgct tccaatgtcc gccagaatct
     1801 tctctcttgc ctggttctcg ttcggtgctc cgataaccct ccggtacggt tcccacttgt
     1861 cgttgatttt acacgcacct ataacttcaa acgtctggtt ctccatgact gtatcactcc
     1921 aaaaacccaa agacttcttc tattcgtccc agttcaaacc cgctcgtaac ggatcctgcg
     1981 atgtatccct tgctgttcgc aaggagcccg gcgcccacga gaccgcttcc catattgatc
     2041 gaaccgaggc cgatcgggag gtcgacaacc cgttcaagac tggcgatctc cgtatcgttg
     2101 gccctcggat ggacgagaat accctgattc gtcgcacacc cggccatccc gacagttttg
     2161 atgcccccga gcgagagccg gatcaccggc acagaaagga acgacccgat ctcttcggcg
     2221 acatcgaact ccatctcggg atggacagcg gcaacacagt cgtttgccag aatcacgttg
     2281 cccgccgcgt tcatggagcc ctcgagcagg aggacttccc ggtattccct gaggaccgcc
     2341 agttcctcct cggtaacgag gccgctgacg accaggccct ggctgtttcc ggcaacaagc
     2401 gagccgatga tgctgcttcc ctggatatag gtgctcacga tctcgacatc gagttctcgt
     2461 gcgagagcct cggtgaacgc ccccggcgcc ccggggtata cgatcgctat gtcctcaaag
     2521 acgcgtgcgt aaacgccgat gttcggatcg ccggcgaggt cgatcgtccc tgtcatttca
     2581 ctcctcagcg agttcggcct gaacctgccc gtcctcgaac ttcatcgcgc ggacgcgaat
     2641 cttccgtggg ggcttctgac tgccgttttc ccagaccttc tcgttgatgg tctggtcaag
     2701 tttgatatcc tcgctcttca tgtgccgcac aagaaacgcc ttgatgtcct tgatagcggt
     2761 gttgcccctc ttccaccggg gtgcacgctt cacctcacgg agagggatga tatagatatg
     2821 ttccttcagt atctctgcca taatctcaca ccttcagaga actccgtctc cagttgcgcc
     2881 tcttcggatg cgacgcaacc gcacggttgg tcctgatcat cacccatgca ggcacgcggc
     2941 ggttctgctc gcatgccttt gccagccgga tcttccggcc cttcattaac ttgctcataa
     3001 ctctcactct cgtagtattc aggtctccct cttaccgacc accagggact gcaggtggcg
     3061 ggaaggaaag agcggcgccg tgtcgatacc gcgagcgcgg atcgcgcaac ggatctcttc
     3121 ttcgtagtcg ccgttcccga tactgcccgt ctccccgtac cgcaccacaa gcagccgccc
     3181 ggcgaggcgt tccacctccg gtttcccgta gccgaggagc ggcctgatgt aggagcagcc
     3241 ggtggtcatc tccaggtgtt gcacctcgga ccgttcgatc cggggaaccc ggtcttcacg
     3301 gcgggtgccg tcgccgacaa ccgcatactc gtgggagagg gtttcgatcg cggtccggtg
     3361 gaccatatcg atcgcatcgt tggggtaacc gcaggagaga agcaggtcga cagcctcctc
     3421 gaggaggtct cttccgagca ccctcctctt gaaggggagg cccagagcgg ccgctgcggc
     3481 ccgcacctcc ggaacatcac ggtcgggatc gaatacgcag gtattcagtt ccacgccgta
     3541 atcgcgcgcg agcatgatcg ccgccagcgc actatccttc cctcccgaga agagcacacc
     3601 tgcttccatt acttccgcgt aatcctgaac tctttcttcg acggtgtcaa ctgcgtaagc
     3661 aggcccttta actgttcgtc agtgatccgt tgtcggaccc tgccgctctg ggccagcatc
     3721 accagttgct gctcgaccgc ttttgcaaaa tccggccggg tcaacttgat ggtgttgagt
     3781 cgctccctcg cttcaggttc caggatctcc atgagtgcgg cgcggacctg cgcatcgatc
     3841 tgctgctggc gtgccgcctc atcttccatc ccctgctggc tgctcatcgc ctgccgctgc
     3901 atctgttcca tcttccggcg gcggagttcc gcaagttcgt cgtctaccat cagtcatacc
     3961 tcctgtcgat cagtatttgg caaggcccgg tgcaatctcg gtagcctgag ccttcaggcc
     4021 gtttgcgatg ttgtcgagga aggatctgcc ggctgcagtg accgtgcgcc ctccgcgggt
     4081 ctgggagacg tatccggttg cttcgagctg ctggaagagt ttccggacga tcgcaccgct
     4141 tcccttcctg aactgggagg gagcggggcc ccggttacgc ttgccgccgt agatggagcg
     4201 cattctctgg gcgccgacgg gaccgtcggt gtagatacgc cggagaaccg acgccgcacg
     4261 cacataccac cagtcgtcat tctcgggggg cagctctttg tgtacccccg tctttgcaaa
     4321 agcggcccag tcggggggct gaatctgcgg gtttttcttg agttcttctg ccgcctgccg
     4381 gatgaacata tcggcaggga tgtcatatac agtcgtcata gatgaacctc actctcgcta
     4441 acagtcttta catattcgtt cagtgatgtt ttatatattt cgaggattgt gcccggatga
     4501 tcgccctcgc cgctccgtga ggacaagagt tcgcccccgg acctcggcga gaaccgcgcc
     4561 ggccgatgcc gctatctcct cgggatcgac ttcggtgttc cggagccatc gtaccttgac
     4621 gaccttgcga tcttttagct ggcgccggat ttcatcgatc atgacactgg tgataccccg
     4681 tttccccacc cagatggtgg gcttgagatc ctgaaatgat tctttactca tggttgaggc
     4741 ctcccgacag gataccgaga ctggtgtccg caggcaagac aggtgatgat cacacggccc
     4801 cgatggattc tgacccgggc gttcgacccg gggaccaggt aggcgctgca ccggcggcag
     4861 aagcggcgtt tcagcggccg ttcaatccgt acccggtggc gcatgccgat cttccgggcc
     4921 agttccacac agcggttgct ccaggtgggg ttttccgggt agaactccgc agcacgtgca
     4981 aagaggatag agatcctctc acgggcgagt ctccgggagc ctgatttacg tgtagtatct
     5041 gccatgtgcg atctgttcca atggtgggtc ttgagattag cggcggaaga atatataagg
     5101 gtttgatccc cggggtcagc ggactcgcac ccgccgaccg gtgacctcca ggcggtcctc
     5161 gcagaaaaac ttcacggcga gcgggagggc cttgtgctcc tcgaccagga tccggtcggc
     5221 gagcgtcgat tcgtcgtcgt ccgggaggac cggcacgcat cgctggacga cgatggggcc
     5281 ggtgtccatc ccctcgtcca cgaggtggac ggtgcagccc gcgaccttca ccccgcactc
     5341 gaccgcctgc cgctgtgcgt gcaggccggt aaacgccggg agcagggcag ggtggatgtt
     5401 catcatccgg cccgagaact cgtggacgat gccggccccg aggatccgca tgtacccggc
     5461 gaggacgaag aggtccgccc ggcagccctg catcgcagcg aggagcgcct cctcgtaggc
     5521 ggccttcgag gggaaccgcg catagtcgac gaccgtcacc gggataccgg cgcctctcgc
     5581 ccgctctatc gcgtacgccc gggggttgtc ggtaacgagg ccgacgcaga ccgccgggat
     5641 atccccggtc gcaatagcgt cgatcactgc ctgaaagttc gacccccttc ctgaggcgag
     5701 caccgcaatc cgttttttgt taccgggtct ctctggatcc ataaataccg gactccttac
     5761 cgggcctcct ccggcccggt ttgaggtgcg aggatcagct tcaccttgac gatccgccgg
     5821 ccccgcatct gcatcaccac gagcgtggtg ttgctctcct cgatcttgac cacctcaccg
     5881 cggcgtggaa tgtgccccag ccggtcgata acgagaccgc cgagactctc atacgaatcc
     5941 gtcagcggga gggccaggtg caggtcctca ttgaggtgct ccacccatgc tcgtgcatcg
     6001 accagataga caccttcttc gatctcctgc acctcgggct cctcctcgtc gaactcgtcc
     6061 atgatctccc cgaccagttc ctcgagcata tcttcgaccg tcacgatccc ggcaaacgac
     6121 ccgtactcgt ccaggacgac cgccatatgc tgcttcttca cctggagttc ctttaagagt
     6181 tcgtcgatct tcttgctctc gggaacgaaa tagggttcgt acatcaggct ccggatcgtc
     6241 gcgctcatct gctgccggaa gaccgctgca aagatgtcct tgacgttcaa gagcccgacg
     6301 acgttgtcga tctgctcgtg gtagatcggg atccgggaga aacccgtctc gttgaagatg
     6361 gagagggcgc tatcgagcgt ggcggtatcc tcgatcataa cgacgtcgac acgcgacgtc
     6421 atcacctcgc ggaccgtcgt atccccaaag cgcagcacgg agtagagcat gtcccgctcc
     6481 tcctcctcga tagtcccctc ctcctcaccg gcgtcgatcc actccttgat ctcctcctcg
     6541 gtgacgaccg gctcgctcac gccgggtctg aaggcgaact gccccttgat acggtccgag
     6601 acccagagca cggggtagag cacctttgag agatagagga taggcacagc gacacggagc
     6661 gcgaattcct cgacatgccg ggaggcgtac atcttcggtc cgatctcccc aaagatcagc
     6721 atcaggatga ccgtgacacc ggttgctatg ccgataccga cgtcgccgta tatgccgatg
     6781 gcgatcgctg tcgcaagcga tgctgctgcc acgttgacga tgttattccc gatcaggatg
     6841 gtgatcagga gggtatcggt cgaccgcttc agggtatcga gcgctgtcgc tcctttgcga
     6901 ctctggttca agagtgcccg aactttcgcc cgggttatcg atatgagggc gacttccgaa
     6961 cttgagaaga agcccgagag aagcagacag ataagaaata atgcgatctc tatggttaca
     7021 aggtctacga ttaccattta ttccacgccg cactggacgg ctgcatctag tctcgggtta
     7081 tccgttgggg ttatatgaat tatgttgcag gcagatgtta taaaaccagc gcttcaggag
     7141 aatatcttct ccagcgcctc caattcccgt gccaggagca caaccttctc ttcagatgag
     7201 aggggcccgg caaagaccat gtcctcgagt tccagggtga ggtacccgtc gtagccccgg
     7261 tcggcaagtt ccgcaagcac ccgggccgcg gacgggtcgt tatggacggg gtggtgaggc
     7321 cgcccgttcc caccgattgc gctgacgtgg acgtttacca tccgctcgat gcagaggtcg
     7381 atgaaccggt ccacctcccc cggggacgcc atcatggcat gccccatatc gagcgtgaac
     7441 cagagccacg gctcccggtc gagcacctcg gcggcatcct ccgcggtgta gagcagggcg
     7501 ttgacccggg gctccaggtt ctcgatcgcc accttcaccc gggtcttctc tgcgacctcc
     7561 cggagccggg cgatgtactc ctcgaaccgc cggtagtcgt aggcgctcgg gtgccgtttc
     7621 gctgtccttc tccccgggtg gacggtgacc acgccggcac ccatccgctc cgccatccgg
     7681 atcgcttcga cggtatactc gaccgagatc tcggcgactc tcgggttgat ggagcagggg
     7741 tttaagtcca gcgacggggc atgaacggtg atcggcgcga gttccgggtg gtcggcgatg
     7801 cacccggcga gttcgtcctc cgggcgatcg cgaagccaga agtgcggcgt ctcgacccag
     7861 aactcgaggc cgtcgaggcc ggattcggca acgtagtcga agatgcagtc gcacgggtac
     7921 tcgtggaaga acatggtcga gacggcgaaa cgacccatac tactacatga tataaaccat
     7981 cggtctaata gattgtgatg atgctcggcg gtcattccac cggtccggat cggttcggca
     8041 ccccgatcct cgcggtttca gaggtctcgg gactcatctg cgacctcctc gacgacgctc
     8101 gcctgcacca ggtctgggtc cgtggagagg tgaccaacta taaggaccat acctccggcc
     8161 accgctattt ctcgctttcg gagcgaaacg gaaggaattc cgcgctgatc aactgcgtga
     8221 tgtggcgcac ctccgcatcc gcgctcggct ttacgccgaa agacggcatg gacgtcctcg
     8281 cctgggggtc cgtagaagtc tacgaacctc acgggagata ccagctgatc gtccgggaga
     8341 tgctcccggc gggtctcggc gagcgccacc tcatggtcga gcgctggaaa caggaactca
     8401 acgccgaggg gctcttcgcc cctgagcgga aacggcccat cccgctcttc ccgcaccggg
     8461 tcggcgtggt cacctcccct accggtgcgg cagtgaagga catcctctcg gtcatcgacc
     8521 ggcggtatcc ggtcgaggtg gtcctctccc cgacggccgt ccagggcgat ggggcccaca
     8581 ccgagatcgc ggaggcgatc cggcgaatcg acgggctcgt ggacgtgatc atcatcgggc
     8641 gcggaggcgg aagcttcgag gacctcttcc ccttcaatca ccccgacgtc gtgcgggccg
     8701 tcgccgcatg caggacgccg gtgatcagcg ccgtcggcca cgaggtggac accgccctct
     8761 gcgacttcgc cgcggacctc cgggcgccga cgccgtcggc agcggcggaa cgggccgtgc
     8821 ccgagcgccg ggaggtgctc ggggaactct ccgggtatga agagaggatg cgttcgctcc
     8881 ttctccaccg cctcgccgcc tcgggaggcg aggtcgagga actcgcgggg cgcatgcacc
     8941 cccggcggct tacacgccgg atccacgagc ggatgcagcg tctcgtcgag catgaggacc
     9001 tgctccggcg ggcggcgctc gcccgggtgc agcgcgaccg gttcgcgctc gcggaggtcc
     9061 gtgcacatat cgtggggaag aacccgctcg ccatcctgga ccggggttac tgcatcgtcg
     9121 agtcgggggg aagcgtcgtg agaagcgccg gggaactcca cccagaagac cgtgtagtgc
     9181 tcttgatgaa ggacggccgg tgcagtgccg tcgtggagga gataacgtat gacagagacc
     9241 tttgaagaga tgctggaaga actgcggggg attgtccgga gactggaaga cggcgatacg
     9301 agcctcgaag agagcatcgt catctacgaa cgcggtgcgc tcctcgtgaa gcagtgcgaa
     9361 gacctcctcg gcgcagccga gatgaaactc accgagctcg gtcgcgaccg gtgaaccggt
     9421 agaaccccgg cgggatgccg ggtgcgcacg cggggctcag gaatcctcgg ggacctccgc
     9481 ctcgagttcg accaccagct cctcaccccg ccggagcgcc tcgatgaacg gccgggggag
     9541 ggtggctgcg acagagtcgg aacggatgcc cacggtccgg tcagagacga agtcgcttcg
     9601 ccgccagacg aggtcggcgg ggtggtccag agtgaacgcc gcgcttcccc gcgaccggat
     9661 ctcgacggtc tcgttccctg cggtaagccg ggtcgtgagc actgcccggt cgtcgcggag
     9721 caccgctttg aggtccgggt cgaggtctgc ggcacccttg tcggcagcga cggcgatgat
     9781 gcagtcgccg gtcacggtca gcgtctcgtc cctcgtcacc tcaaacgtcg tcgggtgggt
     9841 cccctggatc agaggatgcc cccatgcccg cacgatatcc ctcgccttca tgcttaatta
     9901 ggagagggat tctattgcta atgaatgatt agtatcgact gtcccgtctg taaagaggag
     9961 tgcgatcacc agattctccg tgaagccgcc gaactggtgg tgcagtgcaa cgaatgcggt
    10021 caggtccacc ggattccaag gcctccggag cccggcattc tcaccatcaa ggctatcgtg
    10081 agccgtgaga ccgagtcaca ggtctgcagc gtggagatgc tcgccaacga ggtcgtgagc
    10141 ctcggagacc acatcgccgc ggagtgcgga gacgatgtat tcggggtcga ggtcaccggc
    10201 atcgagaccg gagcgaaacg agtctaccac gcagcggccg aggaagtgac aaccctctgg
    10261 acccggggga ttgagcaggt cgtggtgagg gcgtccatcc acaccggccg cacgaccctc
    10321 ccgctctacc agaccgcaga gggggaagag gagttcgtcg tcggggaggc ctaccccttc
    10381 ggcgggcggc ggatccggat atcgcacatc aagctccgcg atggtccggt cctccggaaa
    10441 gaaggcaaga agaccgtggc ccgcagggtg aagcggatct acggctacat tgacgggcgt
    10501 taccagatga agcggtgatc cccgctttct gttaccctcc tgatcaccta aaactgcaaa
    10561 aaagaactga accgcctgcc ctgaccttta ccggggcagg ggttgggaca ggtgccgccg
    10621 gatcgcagcc tccagatctt cacggtggac gagcccctcc atccgctccc tgaccctgtc
    10681 cccctcaatg acgagggttg tcggggtcac ccggaggcta tatgttttga tctgctcggg
    10741 gttcttcaca gcatcgatct cctcgatctc gatcccgaga atcttctcta cctcgcgaag
    10801 aatcggggtc tgctcctcgc accccataca cccttcctgg tagaaactga tcactttcac
    10861 cgccatatat ggcaggaagt ggggagacta taaaaaactg tggggtggat caccccgtta
    10921 tagcattgag cgtgacccgg gcgctgccgt agcggtactt gatgatcacc gagtcatcgg
    10981 tcttgccgac gacctcccca aagcgcagtg acggggtctc gttggtctcg ttgccgagag
    11041 ggatatccgg cgatgccgtc agcccgagcg caatcggctc gcccacttcc caatccgtaa
    11101 ggttaaagcc gagactggct atgtactctt cactcaggtt ttcttcgaag tcattttccc
    11161 cgtaagagag gctgatggtc ttcgtctcgc cggcctgcat cccgacgagc cccacggaga
    11221 tggcgttggt ctcgaagccc agcaggccga agctcacgga tccgttggtc tctgggtata
    11281 agataggaac aggtgcaatg ttctcgcccg agacctgtgc gccgacgggc atctccaggc
    11341 cggaggtcag gaaaatctta ttgttcccct tctcgtactc gctgatgagg aggttctggt
    11401 cggtcgtgat gagggggcgc ccgtcctcgc tgaagatggt gtagccgatc accgcagtat
    11461 tccctatctg ggcggtctgc ttcttctcaa acatcggggc caggtaggtc cccaccatgg
    11521 ccaccgcaac cagcagggcg attcccacat aggcccattt catccatggg gcagtctcgt
    11581 tcttcgaggt tttctgctgt gtctgtttca ttattgtaca atcgcctttt ggtgaaataa
    11641 aatagtattg ttcagtagag aagcaggaga gtataggggt tctgttgcgt tcgcgggaga
    11701 tttgaatagt cgaggcgggg tgttgcatgc gggggatttg ctaatatcaa gaagttaaga
    11761 gacctaatcg cccacttaaa acgtcaaatt cattagcaag tatttttcat ggttgtggat
    11821 taagcactca ctgtatgcta aaagccaaac aagttcttgt ggcagacgat cttacgcctg
    11881 catgggatat aatttgcgac gcattggggg cagttggaaa agatttctac acggtctcta
    11941 tgcgtccaaa aaagggcgcg aaaaagtggt tccacgcatc atcagagcaa aggcgcgtcc
    12001 tcattgaaaa ggcaaaagac cccactaaaa actcacagtt gactatgcac tactggatca
    12061 gacagccaga gtttgaatta atcgctgaac actacaatgg ctatgtgacg gggctcccaa
    12121 cagatattcg acgaacagat agccatgtat catcgtatat tatcacactt atcgccaact
    12181 tgctttaagg aaacactaaa ctcccaggat cagcgtggtt actatttggg tgattgccac
    12241 cctttgctaa tcacttcatc cacccggcgc tcgaaggcgt catcctcaac atagaacata
    12301 tcgatccctg tgcccccttc cttgcgtgtc tcctgcatct gccagaagag attgtagacc
    12361 agttgcccga atcgcatatc ggtatccagc atccagacat ccctgagttt ctcaagaacc
    12421 acatcgatcc gttcgggatc tctttgcgac acgctcattt tactcacctt atctaccgct
    12481 tcctctaccc tccggcgcat ctggttatcc atctcccaca cctctccgca atataccgta
    12541 ggtaacgcag aacgcccata tcaagggtgc gccagacggc cccccgaacc tccaaaatta
    12601 tatttagagg cacaagattt atcgtttcga gataaaaaga aggattgtga accttttaca
    12661 agaggtgttt gattgcgcca tttcatctct tgtgaggtgc tttaagccgt tctatctgtt
    12721 ggtgatcggc ttcatcactg caatggtagg atctgttctc ctaccctacc gagtggcgat
    12781 gcttgtcgat gacgcacaga ctcagctgaa tcttatttcc aatgtccttg aactcccgtt
    12841 tccaaaagtg gttgtggggg tagctgcaat cgctctattg ttctacgtta tcaaaaagtg
    12901 attttttact gatttgactg aaccgccagc tcgatcatcg tcatccagcg gttgccggag
    12961 aggttgatcg ggatctgggc catctgtgcc ggggtcatcc cctcgatccc ctgatggggc
    13021 cggatgtagt tgtagtaaac ctgcatcccc gcacagaact cgtttgctcc gatctggtcc
    13081 ggcaggcttc gcatcacctt cgtccgctcc cggaaggtgc cgttcaaccg ctccacgatg
    13141 ttgttgttcg gcttggtctc gaagtccttc agccggaagt gggggttctg gatgaacgcg
    13201 gaccggtcaa agaacacgct cctaaccgcg tactgatacg cctgtaggcc gtccgtcacc
    13261 acgaggtcgg gtcgccgggt ggctgcatcc ttcgcctgct gtaagggctt ctgtgcgtcg
    13321 gtaacgtacc ggtccttcgt cacctgacag gcgaggaggt agcgggtctt ggcgtccatg
    13381 acattccaga tccactcgta gtaccgcttc tccccctctt tcttgatgaa gactgtcatc
    13441 tcgtccgagt gccagatctt gccgacatcg gctttgtgct tctccacata cgtgccgagg
    13501 agtttcaggt acttcttgat ccagcgcatc ggggttgtct gggctacctc gatgtcgtgg
    13561 aactgcttca ggtgatcagc aatcttccgc agggacaccc ccttgaagta gagatcaagg
    13621 gcgagggtga tggtcttcgg gtcatgcttc atgctcttga accccttgtc cggggtgaac
    13681 cggtggccgc agtccttgca ggtgtaccgc tggatcttgc cgaaccgggt tgcccggcat
    13741 ccatcccgga ccacgctgag gctctgacat gcggggcaga gcacttcggg gctgagggat
    13801 tcgatctcct gatgcgcggt ctcctgctcg atgtcggcct tcagcttcag ggagaactcg
    13861 accgcgtaga tatgcttgca gatctgcttc cggtagacgt gatccgggca ggtgcaggac
    13921 cacatgaact gcttggtgcc gtagccctcc ggccacttcc gctctacccg gtagtaggcg
    13981 tcagacttcg cctgcgaccg gacgtaccag acgttcttct taatcgcttt gatgttttcc
    14041 ggctcggaga gtatcgcaaa ccccttgagt tcccgcacgt tggttaactg ttgcatctca
    14101 attaccctca ttttattgat tgtaaatcgt acgatttatt ctattatctt ctattgagat
    14161 aaacttttcg ggagttaatc gtacgaacca ttgtgggaaa ctattatatc tcaatcgtac
    14221 gctatactat agtaattgtg agtgtggagc gtacttatgg cagaagatcc gatgtcaaat
    14281 gagccagttc ccgatctgga cgtggatgtc atcaagaagc gggcaaagat cagttatgac
    14341 gggcggcagc tcatgatccg catcccccgg gagatcgcgg agttctatgc gctggagaag
    14401 ggagatcttg ttgagatgat ggtcgagatc tccgcagagg atacgcgacc gagccgtgag
    14461 atcccgatgg aactgcgtgt ggtggagaag ggataggcgt gacgctgaac gtctacgagc
    14521 aggcaatcct tcggctcctg aagaccgccc ggagatccct gacgacttcg caggtggccg
    14581 acaagatcgg catctcctgg gagaccgcga agaagtatct ggaactgttg caccggaacc
    14641 ggtacgtttc catgcagcac aaaggaaacc ggatctattg ggaaattaaa tgaggcccct
    14701 ttgagattgc tatttgaggg agcctattca gtaactgatc ttccctctat gaccccgatt
    14761 tcctcttgag tcagcccata gagtttgtac acgatttgat cgataagttg atccgttgca
    14821 tcaattttgg ccagcatggg ctttagtttt gcaatagagg aattgtactc aaattcaagg
    14881 ctttcacgat ttgccctact ttttagatta taatcatcac gcagtttccg tttattcttt
    14941 tcaatgaccg caaaaaaggt tttaaaatta tttttggagt caatagagtg atacccctga
    15001 attacagttt tattagagag ggtatcaact ggtaccccga tctcgctctc aatgtacttt
    15061 aagaaggcta cgatctcagc gttctttgct ttattcagaa ttgctatttg ttctgcaagg
    15121 aacgcgagga gatcgtggat tacatccact tcttctggtt gtgcaatgtg tcgagtatgt
    15181 acagatgaga gaagggcgct ggggtcaaag ttttgaagat atgtattgta caattccatt
    15241 aattcagcga tcaatgcttc cctgcgtgga ttctgggttg taaagtggat actggggatt
    15301 ggaagttgat gaagcatata tccgtcaata tctgtaattg gaaatcggat tttgaaataa
    15361 tgattcataa cttttgagtt gagtaaacta catataaatg aagcagaata cgctgtctct
    15421 agcattttga ttagtataaa tcgattacta aagaagatat tgtctgctgt gcttgctgca
    15481 accaatcgcc gttttaactt tggttttcgg gcagcgtgca agacaatttt tatacctaca
    15541 tgaaggggtt ggtgttttga ccagagttct tctggaatat acgatccgcc ccattttatt
    15601 tcgtatctcg ataattgggc tgcactttct atcacaggca tatctgtaga gaggggcgct
    15661 gatgttttct taaatttttc aacggttgat ttataaatcc catcggacgc atgtccaatt
    15721 gttcctaaat gaatcgaatt tgtctccaaa catctacaga agttgtagtt tctagcagtg
    15781 tcatggacag acatttgatg aagtgggcga tcaaaccatg ttttttgatt tgctgaatct
    15841 tcccatagtg aatctgtcaa aaactgtggg tgtcccaata gttcatcatt gaccacacga
    15901 acagatataa tgccatttag aagttgctct atgctttgtt tctctttaac aaattcaaaa
    15961 attacaggga ttgtgtcggg tgcgtctttg aatactccct tgcacaaata atgaagcgag
    16021 gttatccgat agtgaagaag aataaatttc cgcaatagac agctattttt attaattaac
    16081 cacgtacttg gaacaatttg gccaataatg cccccttttt ttgccaaaca atagcactgt
    16141 gcaataaata gattgtaaaa attaatctcc ccttctcgaa tagattggaa ctggacatta
    16201 agatattgtt tttcaggtgc ttgagcagat attttccccc aaggaggatt cccaagcact
    16261 gcatcccaac cagcattctc acgaagtttt cccgcatcaa aaaatatctc aggaaactca
    16321 agttcccaat ggaaaaagga cttctctttt cccattgtgg tggctttctt aaaccactgg
    16381 taccgagttc gggttttcca ctccgcgtcg tttccgtata aagaacctaa tagatcataa
    16441 tagtaacttg caggaatccg gtaatctgtc ttttcgatcc ggtttccaaa gtaaaaagaa
    16501 gtattcaagt cagcgatctg catggccttc tggtactctg gtagattttg caactcttga
    16561 aacaactttt ctttcgtttt gatatcgtcc agattgtcgt ccccagtttg ctcaatcgta
    16621 ttgattttgt tgagtatttt tttaacgatt gccggagaga taatcggtct ttgggagggt
    16681 ttgggggtac tctgtagatc cccctcatgg taccacgcaa gatccgtgat ttttgctcca
    16741 atgaggctgt tgccctgctt taaccgatgg tcgaggaagg agaggggttt atctttgctg
    16801 atggtggtca accacaatcc gacttttgct aattccactg ctagttcatt gagatcgaca
    16861 ccatatatgc aatgagatac gatctcacgt cgtgcccact cagtagtgca tgttgatgcg
    16921 tcactgatcg ctccgttgtg gagatctttt tgtaccgctt caaggagatt ctgagcgagg
    16981 tgatcaaccg ccccgaccaa gaaatgtccg ctccccattg caggatcaag gacttttatc
    17041 gaaagtgttg cagcgacgta actcccattc ttatccagtg cctcatgcca ccgctcttca
    17101 acaatggggt caattgtgct ctttacaatg taattgacga tataatctgg agtatagtat
    17161 gatcccgatg acttccgttc tcctttgtca gtgacgagga agagatgccc tgtgctcact
    17221 cgatcattct catcaaattc ggtaaaccct ttttcctttt tcttgccctt gttgtactct
    17281 tcaagcgtca cccagcaccc atcgctcgcc acaagatcag attcagccag ttgaagccta
    17341 tactcaagca acccctcgta gatactgccc aggtgcctga tctcaagggt cgagtagtcc
    17401 acaaagtcac gagacccctc attaggcttg cttctagaaa gaagatcaat tgcctctgca
    17461 aggtaggagt cgcctatccg ccactcttcg agttctgggt gtttttgagg atcaaagaga
    17521 cccccattgt aggcggggat atggaacagg tctttcggga tgccgataga tacacttcct
    17581 tcatcaatca gttggaatag gtcgcgtagg ctggtgtaaa gggagcgggt gcttgggagg
    17641 tagcgcagag ggattggtcc ctctgttttt tccttcactt ctttcttcaa gcgactgaaa
    17701 cta
//
LOCUS       DAJB01000102            3539 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_30239, whole genome shotgun sequence.
ACCESSION   DAJB01000102 DAJB01000000
VERSION     DAJB01000102.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_30239"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1435..1572
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          687 a          967 c         1039 g          708 t
ORIGIN      
        1 tagcgttcga tcgcctgcat ggtatggggg tggtgcccga ggatcagggt ggcccccgcg
       61 tcgatgagcc ggtgggcaag gtcgatctgc tggggggacg ggtagtaggc catctcggcc
      121 ccccagtgca gcgagatggc aatgtggtcg caccggcccg cgagagtggc gatgtccttg
      181 aagatatcct cctcgaccag ccggttgact gagacgtcgt gggatacggc gagtttgccg
      241 atcgtgtagc cggcaaaccc gatcgagatg ccgttcctct cgaggatgac cggctcctgc
      301 cggttctctg tcgccgatcc cccgatgggg aggacgccgc ggctatcgag cgcattcagg
      361 gtgtttgcaa agccttcggt tccgaggtcg ccggagtggt tgtttgcaac gctcatgacg
      421 tcaaacccgg catccaccag gtatcgggcc gcatccggag acgtggagat cacatgatgc
      481 ttctccgccc gctcccccgt ctccgagaga gcggtctcca ggttcccgaa gaggaggtcc
      541 ttatcctgga ggatatggtg gacctcctcg aatggatact ggttgttccc cgtccggacc
      601 catatatctc ctgctgctat taatctgagt ttctgcatgg tttctcacac cgaaatgccc
      661 caccggtcca gcggcaggtc ctgtatcagg aactgatact tgccgcgccg tgtgcggggg
      721 attatcccga cgaagcggat ggtcacatcc acgtcgcctt caaatttttt gttgagttcc
      781 cggagaatgc gttccgtatc ccggtcgtta tatcccggtt tctttacgat ccgcatcagg
      841 acttctcctt ccctcttctg gtaaaactga aactgtgcaa cgttgtcaaa gacatcggag
      901 tgcatgttca ccgccgtcat cgatatgaac cggcgatcct tcgtcacgat gaactcctga
      961 agccttccct caacccggtc aagcatcggg taggcacggc cgcaggagca gggggtctcc
     1021 ccgagaaccg caatgtccat ggtccggtag cggatgaggg ggcagatggg gtttatgagg
     1081 ctcgtcgcga ccacctcccc catcgcgccc ggcccctcca cgggcaggcc gtctctcccg
     1141 atcagttcga cgatcccgta ttcggggaag atgtgatacc gggtgctctc ctcacactcg
     1201 ccggcgagca ccgtctgttc ggagttcccg taccaggaga agacccggca cccgaaggtc
     1261 tcggtaagca ggctccgctg ccaggggtag aggttctcgg agccgcagag gaccgcttta
     1321 acggtcggga agggctcgat gccgtggtcc accatatacc gggccaggat catggccacc
     1381 gatggatatg cctggatgaa gcggggccgg aattttcgga tctggtcgat gtagnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnttctcgta gtagaagccg acggggatac ccgtggaccc cccggtggtg
     1621 acgtactcga aggctgtctc cgggtagttc cgggctttta agtcggcgag gttgttctgc
     1681 aggtccgccc gggtcaggaa gggaaggaga gcaaggtccc ggggcgtctg gatatccccg
     1741 ggcacgagat ggcgctcttc aaagacccgg cggtagtagg ggacgttttc gtaggcgtga
     1801 tcgagcagac gcgagagcgc ctgcatctgg taggctgcga gttcttcctc gctccaccgc
     1861 cgggattgct gaaggaggga gtaagtctgt ttgtacgcag agtatgcggg gaggatgcga
     1921 agggccctcg ccgtcacaga attttcaagc cggagagcct tcaacccccg atgattcatc
     1981 cgcacaccca ttgccactgc tgatgttagt acttcgcatt aaacgttttg ctcacggtga
     2041 ggtgacgtaa caccgttaac acggtggaga cggcaggcat cggtggagat tgcgtctggt
     2101 tagaagaagt tctcggagta cgggatggag accagatccc ggccggatgc acggcaaaag
     2161 tgtttcagga actcgggaaa gagatttttt tccgatatga ctacccagat gtcaccgtac
     2221 gcacccgaac cattgccctc ccacgagcga aacgatacgt cgtccatggc gagaaggcgg
     2281 agtgcaacca tgcccctacc tcacgtgtag gcgtatatat aggttcccgt cggggtcagg
     2341 cgccgtgaac ccggggtgcc ccgggtcttc cgggtatctc ccccggggtg gggtggccat
     2401 gtcctgcctc ccggcggatc cggagaaagg tatatatgga cccgggaggt gattagaggt
     2461 cgcggagcag cagagaacgt gacagcgccg aggtgacgga aggtatgacc gtgagcagag
     2521 ttgacgacag agatacctgg gacgccttca tcgatgacag cccctcagga cttctctttc
     2581 ataagtggga ctacctgcac ctgacagcaa agcatacggg gagcaccctc cttccgtacg
     2641 cggtctataa gggggaagaa ttgatctgtg tattcccgct cttttccagg cgaatgcatg
     2701 gcgttagcgc tgtcttctcc ccacccccgc tcacggtgat acctcacctc gggtgcgtga
     2761 tgagcaggag ctttggggag cttaaacaga gcaagaagga gtccctcctc gggatggtgg
     2821 caggggagat ccaggacgag atccggaacc tctcaccgaa ctacctctcg atagccttcg
     2881 tcccggagtt caacgacgtc cgccattacc tctgggaccg gtgcgacgca cgggttcggt
     2941 atacctacac catcaacctg gatcgaacgc ttgaggtgct ctggaacaac ctccactaca
     3001 aacttcggaa taaactgaag aaagaagccg gggccggtct ccgactggag aagacgagcg
     3061 atatctccac gctccaccgg ctccttgccg accgttacca ggacccgtcg cttgatattc
     3121 cgctcatacg gcaagattac ctcgaagatc tcgtgcgtgc ctacccggat cggatcgggg
     3181 tatactacct ctacgatgcc ggagacgagg ttgccggtgt ggtggcaaca caggaataca
     3241 agcggtttct gctctggatg ggaacaccgc ggctcgatgg cgctcacgcc gggaacgagt
     3301 atctccagtg gctcctgatc cagcgtgcaa aggccgaagg ctaccggacc tttgagaaca
     3361 tgggtgcgaa caacccggac ctggtcttct tcaagtcgag gttcaacccg gatctcatga
     3421 tgtacttcga gatcgagaag aaagacaccc tcggggcagc ctctgagtgg gcgtacctcg
     3481 ccttcgtcaa aagactgatg atggcgaccg gcagggtcta gtgcatcttg tcagtttat
//
LOCUS       DAJB01000103            6290 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_9889, whole genome shotgun sequence.
ACCESSION   DAJB01000103 DAJB01000000
VERSION     DAJB01000103.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_9889"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          920 a         1911 c         2082 g         1377 t
ORIGIN      
        1 gtgaggatcg gcgtcagggc gaggacgttg ccgctattcg gcattgcgat cgcctccgaa
       61 gagcaggtcc acgatcgcct ccaggcccgc cggatacggg acgtcgaacg cccgctcgaa
      121 gacctgtgcc gacgtcggga actcggggat catggtttcc acccgttgaa ccgcttgata
      181 ttcccagcct cctctgcggt cagttcgagc cattctgggt gctccacgat cgccgcaagg
      241 acgtctgcga ggtgcagttt catccgcgcc cggtagtaga tgtcctcgtt cagcagggtg
      301 agcgtgatgt ccgtcgcctg ccgcatcaag gtctgtttcc ggcgtgcgtc gttgtcggtg
      361 tggttttccc ggatgctctc cttcatcgtg cggttgaggc acgcaagaag cggatcgtcg
      421 tagacgaaca cggcgtccgg gtccttgagt ccggcacggt atgccgcgct gacgccggcg
      481 caccggtact tcaggaaccc ctgccacgcc tggtcgagga gtttttcgag gatcgtgttc
      541 ttgatcgtct tctgaaagaa catcccgagc ccgccgacat agcagttgtg cgcaaacccg
      601 aaccgggtgc cgttgtgcag cgcctgctcg gcctgcgggt cgcgggcgat catcgccgct
      661 ttctcttcag ggctcatgaa ctgcgaggcg aacgattcgg acacgattat ccctcgaagt
      721 aatgcggctc gcggatcagc cgcccggaca cgacgacgtt gccggcgccg gtgccgacgt
      781 tcgtgacgcg gagcaggtac ttctgcgtcc ggtcgaggta cagttcgggc agcgtgccga
      841 tcgcggcgtc acctgcgccg acgtccccga ggacgccctg ccagacgata tctgcgggca
      901 gccgggctgc gatgtaggcg atcggcgcgg cgaggttgcc ggatgcgtcg atcgtgggcg
      961 tgtggtagat ggcaggcgcg ctgttcatgc tcgcatcgcc gttccggttc aggccgtggg
     1021 cgacgagttc ggtgccgtct gcggtgacgg tggtgccctc gaagacctcg acagagacgt
     1081 ctgtgccggt gtcgatatcg acggcgagcc ggagagtgtc tgcggcgtcg ttcgcgctcg
     1141 ggtcgaggag gatgtaggcg atgccggctg ccgcgagcga ggcgaaccgg tgcgagaacc
     1201 ggaacgctct gccctgctgc acggcgtgga ggatgtcccc ccctacgatg tgcctgccct
     1261 cgtcgtcaat gatcccggcg acgccgtatc ggttaactgg ggtgaaggcc atgatgcctc
     1321 cagaaagaca gagagggtta gctctccgtc atgtagcccc acatcacacc gaggttggta
     1381 gacgacgtcg tctgccggag gttggaaaac ggcgtccgcc agatatcgta ctggtagacc
     1441 tccgccccaa gcttcggatc gtcgtactgc cgggccttga tctgcccgac gacattgatc
     1501 acgcccggga ccttgttgca gaccgcgaag acctgcgtgt tgccgctcgc gtcgaggttg
     1561 tcgatgtccg tcgacatgaa gaccgggatc tgcagcgtcg ggtggattga tgcggccccg
     1621 cgccattctg agaggttctg gattgcgacc ttgtcaccga cggtgtctgc gtagtattcg
     1681 cctgcgaggg tgcccatcac gaacgcggtc gggcggtaga tccccattgc tgccgacatc
     1741 ttgccgagcg cgagggtggg ttttgcgctc gcccagttgc cgaggttgcc gttcccgtac
     1801 tgctgcgggg tggtgtcgag cttctcggcg atgagtacgt tcaggttcgc ggcaagtgcg
     1861 ccggcggcct gctgctgctg gagcgtcatc gggctgccga catcgctctc gatcatggcc
     1921 tcgtcggata ccgcgagcgt taccctgtcc ttgaggagct cgacgtcgta ggaccccggc
     1981 gtcgcagatc cgacctggga cacttcgaac tcttcgagct gcgaggcgac cgggacgttt
     2041 ccgagcatcg ggatcgtgcc cttgagtttg ttgtacggca gcgtcgggca gattgccggc
     2101 gatgcgaggc tcgtcatttc cagcgcggcc atgatgaccg gtaggatcat ccgcttctgc
     2161 gcccagttgc cgctgatgtg cagctctccc agtactcctg tgtcagccat ttcaagcccc
     2221 ccatacatct acgacgccga ccgcaccgtc tgcggcgcca acaaccatcc tgccgagcgg
     2281 cttgatgtag ttcccggacg ggtcggtact gcattctttg accttgccgc cgctgccggt
     2341 gacgactgcg ccggtgtagc agttgccgct gcaatcgacc gcgacctcgc ccttcgtgac
     2401 ggcgcgcccg gttgccccgg attcgatggc ctcgatcgtg accgcgaacg gtcctgcgcc
     2461 gtcttcagca caggtcgcca cggttgatgc cgctgccggc gtgacggtgg caaccgcgcc
     2521 ctttgcgacg tctgcggacc cggtgaattt ctgcacacgg gctccggtgc tggcgatctc
     2581 gcctgctgcg tatgccattt agaccacccg gatcccgaac ttcttctcga aatcctgtgc
     2641 gaggttcttg aactgcacct cgtccttgtc gccgccatcg ccgggagtgc ctgcgacgcc
     2701 tgcgccttct gcggccgtcg cggcgggctg cgtgttcttg aacgcgatca gtttgagtgc
     2761 gaacgtgccg gggtcggcct cgaactctgt acgggtggcg gcctctttgt cgccgagcca
     2821 gccggccggg aggttcgtct tcatcgctcc ccacttggcg tccttctcgc tggtcgcctt
     2881 cgcggccttg agcgtctcga tctcctgctt gaggttggcg agttctgcgg tctgtgcttt
     2941 cgctgcatcg agttccttct tgaggttctc gaactctgtc aggttgacgt gctgtgtggg
     3001 ctcgcgcttg agtgcgtcga ggatgcccct gagcagtccc tttgattcgt cgtccatgtc
     3061 ggattcctgt tgtaaattgt ggaacattgc cccactgtcg ttcggatagc agttcggaca
     3121 cgctccccgg tcgaagacga ggacgtggtt cggggtcacc tgccccgcga tccgggttgc
     3181 ccccgggagc ctggggtccg ggacctcggg tgacgcgagc ccggttgaga gagagagcgt
     3241 gtgcgccttc gccttcgcct cgatcgcctt gtcggtgaac gcgatcgccc ctttcagggc
     3301 cggctctccg gcctctgcta ggtgagcggt tgacacgttg ccgacgacgc ggaacttcgg
     3361 cggaagggtg ccggcgagca cgtcctcgaa tcgcgggtgt tctgcaggtt cgcccgggtt
     3421 cgtctcgacg tagatcaccg gcgcggcatt ccagcgctcc acggtgggcg cgaagtgcgt
     3481 cgcatcgtag tacacggatc tgccgttgtt ccggtgatag acgttcagcc gttggagcgt
     3541 gacgtcgtgg aggttctcaa aaacgttgga gtgtgcaata tctccacgag acatattagg
     3601 agtatgcgcg ctcatacggc atataaaaat ttgttggctg aaaaagggca atctggctat
     3661 ttctcgccgc ccgtttactt attaggcaca ggatcggcgt gtaaatcttt atgcctttcc
     3721 aggtatacga ggaacccggt cacgccgccg gggccgtgga acttcgacgg cagtccgtgg
     3781 gcgtcgatat ggcgtttgag ctgcgtggcg aggtcggctt cctgtgtcgg ggcgatctcc
     3841 ggtttgatgc ggcgctggta gttccggacc atgtcgcggg tgatgtcgct cccggcgaat
     3901 tcggggagtt tgacgatgtc cgccgggaac ttgtcgaggt tttcccggat cacccgctgc
     3961 tgtctctcgg tgagggtcgg ttttcgggtc atgcttcgat ctcctctgcg ttcagggtca
     4021 cggtctgccc gctctcgatg gtggccccct cctcctcgac cttgtagtct gcgtcgacga
     4081 gcatacatcg gcagttcggc tccccgagcc acggggcctg gtcgatgcgg tagatcttgc
     4141 cgttccgtgc ggcgtggtcc gggcgggcgt tgccgtcgtc cacggcgaca tattcgagat
     4201 agtggacgcc ggtcttgcgg taggttgcca tacgtgcgtc ggtgcgcagc ttctgcgcct
     4261 cggtgcgggc ggcggtggtc gcgttgtggg cggtgccgtc gaagtatcct cgcagctccc
     4321 gggtgatctg cgtcgggtgc atgccgttgc gctgcccctc gccgatgatg cggaggatct
     4381 cttcctggtc gcgagcagcc atgtctgaga gccacggctt aaacgtgcgg cgggtgctga
     4441 cggagactgt gccgtcgcca ttgtcccgga gcacccgctc gatgatgtcc gtgccgccct
     4501 tcttgacctg ctcccggtag gcggcgacct gcggctttgt gatgccctcg acgatctgcc
     4561 gggtgtactt gtctgcgacc ttgcgctgga gttcgccgag cgtggactgc accgcgacgg
     4621 ggacgatcgc ctctgcctcc tcgaccagag cggcggcgat cgcggcctgc cggtcggcga
     4681 ggacttcctc gattaacctc tgctgctcat cggtcggcat ctactcccct tttgccagga
     4741 tgcgctcgat cgcctgcagg ctggcctcgt tcgcggcggc gatcttgcgc tcggcggcgg
     4801 aggccgtgcg ctcttccttg cgggtgaacc cggcgaggtt gccgaagggt atcagggggg
     4861 cggcctgcgc gtaggtctgc tccagcacgg cggcgacctc atcggagtat tcgccgaggt
     4921 tgagttcgga gaggttccgg cggatctctt caggggtgat agacttcccg gcgatcccga
     4981 cctggatctg ctgcgcgaca acctcggagc ggtcgagttc ggggcgcttg agttggattc
     5041 tgacgtaaac gtcatcgtag ccgttcgcgg tcaggagggg ctggaggaac gcctcatact
     5101 gctcctcgat ccacgactgc gtgccgccga tgaagttgtt ccagatccgc atcgatccgg
     5161 cgtcgctgcc gccgatgctg ttgccctcac gcttgagcac cgtcgtcggg ttgaagtacg
     5221 cttcgagcca gtgcacgagc atctccagcc ggtcctttgc cgtcgtggac tcgcggatct
     5281 tgacgtccgg gaacgtgatg ccgtcgggga ggaaaaagcc cgtgtccttg ccccatttct
     5341 tgacgaagtt gtcgccccat gtcttgaggt cgggcgtcat aatcccggtc acctgcggga
     5401 agatcagggg cgcgccgacg cggtgcacct gctgatcggc ggcgtggttc gcgtggtcga
     5461 tggcggcgat cacagggtag gcaggtaggc agtaggcgac gcctgcaggg aagggggtgc
     5521 tcgggtcgcg gatgatcgag acgttcttga gttcggtctg ggcgagggtg tcgtcggacg
     5581 actgatagac gcgggtgcgc tgctccttct tatcccagac gatgcctggg acgagggggt
     5641 ttggcggcgc gatcatgccg aggccgtgcg gagcctgcct aaacgagatc gcgggcaggt
     5701 cacggatctc gtcgatctca aacttgccgc cccggaactt gtacccgggg ctcttgactg
     5761 agcagccgaa gcccatgcac tccagccagg agaccttcat gctcgggtaa acgcctgctt
     5821 ctgcggcctg cttggcgatc catgcggaga ggtcgtcgac cttctccccc tcggcatcct
     5881 cgacgtagat ctccggctcg cccgggaaga cctgcacctg catgttgatg acctgattgc
     5941 ggagatggat gttcccggtc agatacttcc aggcggtctc cggggtgatg ctcggtgcgg
     6001 tgtaggcgtt ctccgaggac tggaatgaga cctcgccctc ggctttcgtg ccgcgtgtga
     6061 cgcgagtttc ttttgctacg atgtctgctt ctgccattta tcttccaata atcgctacga
     6121 tagtcataag tgcgaagagc agcccgaacg cgagcgcgaa ccacgcggcg aggctcatct
     6181 gctgcccccg aacgagagcc attctttggg gatgctgccc cccttgcgct gcccgaaatg
     6241 ggaatacgcg ccgtagcgca tcgcgtccat cgcgtgatcg ttgaacttga
//
LOCUS       DAJB01000104            5600 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_99510, whole genome shotgun sequence.
ACCESSION   DAJB01000104 DAJB01000000
VERSION     DAJB01000104.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_99510"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1027 a         1747 c         1732 g         1092 t
ORIGIN      
        1 gaacgtgaca ccgaccccga cgatgccagg gcaggagacc atcgacctcg agctgtatga
       61 aggctggaac ttcgtctcta tcccgagaca gctctctgca ggtaacgaca ctgcagcatc
      121 ggtctttggg gatgtggaca ccgatggacg gccgatctac acctttacct cggcgaccgg
      181 cttcactcct ctgggcgaga atgagactct ggaagtcctg gagggatact gggtctattc
      241 gaccgaggac acaaccgtgc agctgaacct cagcaccaac ccggtgaggg cccctgcaat
      301 gaagacgctc accccgggct ggaatgccat cggctactcc gacctgacag agcgaactgc
      361 ggatgaggcg ctcacatcgg tggaagacag ctgggtctat gtcgtcggtt tcgatgcagc
      421 gaaccagagc taccggccag cgctcatcaa caaccagacc ggggcccagg gcgagaacca
      481 gagactctcc ccgaccgagg gctactggct gttcatgggc gaggatggca gactggctgc
      541 catcagcacc taaccctttt ttggcaattg cgtcgttgag tatttccgct gacggcacct
      601 cacggtgaga ccgggtgaan naaaaatgtc cccttaaaag ggggttaacg ccggaaaccg
      661 tcccgacgtt ggccgctcac tcctgagctc ccgtatactc ccgcatcagg gcgtcgccat
      721 aggattcttt cttcttcgtc gggatgtagt tgaatgcccc ggcccgtctg aggagttcgg
      781 cgcggatcgc ggcatcgtca gtcaggtgca ggtcgcgcac gccggcgacg atcatctcga
      841 gctggttgat gccggtctct tttccgtcca cgaggaccgt cttgatcttg atcgcgtccg
      901 gccggatacc gccgcagacg gtgcagtagt cgccttcctt ctcgttggtc atctgctcac
      961 tcccggatca tcgtcagcga catcttgaac cgggttatgg ccgagagggc gtgggggacg
     1021 tttgccggga agatgatcgt ctcgccctcg cccatatggt gcgtctcgcc cgcgacccag
     1081 acctcgcact ccccgtcgag gatcgtcacc accgcgtcgt agggcgcggt atgctccgag
     1141 agcccctcgt ccccgtcgaa cgagaagagg gtgatgctcc ctgccggctt attgacgacc
     1201 atcctgcttg ccacggtccc gtcctggtag gcgacgaggt ccgccagccg gaggactttt
     1261 ccccgtaact ccgcacgctt ctcttcagac atgatctaac ctttcagtgg tattacttcg
     1321 ttcttccacg ggcaatctac ttttcgcatt cgcccggcca tatacgagag ccccttccac
     1381 cgggcaggcc tccgtgcacc ggccgcagag gtagcattcc ccgaggccgg cggcggggtt
     1441 cgcctccccg gtcgggcatg cccgttcgca cttccggcat cctatgcagg cgtccgtccg
     1501 ccggagccgg tagaggctct ttgacgccgc cggggcgagg agggcgccgt acgggcaggc
     1561 gtagcggcag aacggccggt agacggcggt ggagaggagg acgatcgaaa gaaagacgaa
     1621 aaacgatatg ttcgcgacgg cgaggtggaa gaatgccggg agacctatga gtccaaggag
     1681 gttcaccgag agcccgagcc cggcggcgag gaagacgacg aagacgccag ccctgaccgc
     1741 caccgggaca cggctctcgg tgttgctgtg tttgttcacc ggcacgaggt acatgagttc
     1801 ctggacggct cccaccgggc agacctggcc gcagacgacc cggccgaatg cgagggcgag
     1861 gatgatgaag gcgagaagcc ctataagtgc cgcagggagc gccgccgcat cccggaggat
     1921 gaccatctgc agctgatacg ggaagaccgg cgcaaagacc aggaacccga ggagtgccga
     1981 gacgatgagg aacggcactg cacgccagcg ggtgaaccgg ccggagtacc agagccaggc
     2041 gagggcgaca aatgcgagga tgccgtatat gaagcctacg tacggtggga tcgactcgac
     2101 cattatactc tcacagcagt ctcgtcccgg tcgtcggccg cgggaccttc accacgagca
     2161 cccggaaggt gcggtcgctc tcgttgatcc agcggtgcgg gatcttcgcc gggctctcga
     2221 ccagatagtc cgccccgacc tctgcgcgct catccccgat ctcgacggtc ccggtccctt
     2281 cgaggacgta gaagaagacg tccaccgggg ttatgtgctt cttgagcgat tcgccggggg
     2341 cgagcgtgat caccaccgca gtcgcatgct cggtatcgta gaccttccgg gcgtcgacat
     2401 ggtgcgggtt ctcgccgatt ggttcctttg ctacatcaac gattttcatc gtgttcatct
     2461 ccagaggtta tcatggcact catcccttat aattcgtcca gtagttcgcg aggctttccc
     2521 gctgcctttg ttcccggcgg cgggcccgca tctccgggta ggtcggttcc gtgctctggg
     2581 ccgccggaca gtaggggcag agggcgtagg gcacgtcgtc ggccatatcc ctggccgggc
     2641 agaggtaggc cccctcccac tcgtcgacga tctgcccgcc gggaaacggg gtgccgaccg
     2701 ggtgggcggg ctcttcctgg acgaagatcg tgaacgcaga gaggaggtac ttcaggtaga
     2761 ggtcccgggg gtccttctcc cgtgcctttg cggtgcaggc ctccgccacc attgagcagt
     2821 aggcgtcgag cgccgggtcc gggggctcgt ccatccgctg gccgccgccg ttccgggaga
     2881 ggaggaggag gcggtgatgc gctgaaaaga tctcttccct gacgctttct aggagacggt
     2941 cgcggtaacc ctccgggagg tggctgacct tccgttcgaa cctggcattc atcgcctcga
     3001 ggtcgtgcag gtcgaatgcc gctacttcgc cagcgatgat ccggaggagg tctcccttcg
     3061 tctccgcccc ctgcatccgg cggcacgccg tcctgatggc ctccgccgat cctggctcct
     3121 cgtgctctcc ctcctccccg gaatctccgg cgaaggtgtt gatgaggagt gagcagacct
     3181 cgccggggcc ggctccgggt gggtcgcggc cttcccggcc gatgagctgc cggatacacg
     3241 cgaggatcgt tacgagccgc atggtcccat ctgttctccg gatgagcatg ggacgcggga
     3301 aggtttaact ctcgtgtaac tctcatagta acaagaatgg acgaccttgt ccggaacctc
     3361 acagcgctcg ggctgacgga gtacgaagcc cgggtctacg ccgccctcgt cggcatcggg
     3421 gaggggagcg cacgccaggt ccacgaggtg agcgccgtcc cgcgtccgag ggtctacgac
     3481 atcgccgaag ggcttgccgg ccgggggttc atcaccgtcc ggcgggggga gcacccccac
     3541 gtctatatcc cggccgagcc cgccatcgtc atccaccact taacgagcgc cgtcgatgct
     3601 gcggcgacag cggcggtgca gggtctcgat gccctctcgc tcgacgcccg gtcgaagaat
     3661 tcgccgatcc ggtacgtgca gggcgagtgg agcatccggc gccacgcaga gtcgcttgcc
     3721 gcgggcgtca cccgcgacct tgcggtcgtc tgcctggact acgggcagat cggtgaattc
     3781 gcccggttga tcgccgatgc gtcgcgtgac cacccggtga gcgtcctcct cccgaacggg
     3841 atgcacggga tcagaaagcc gctcgggaac gcatcgctct acgttccgaa accgttctgc
     3901 accttcttcc aggagaatat attcgataaa gtctactccg gcccgctctc ggtggacggg
     3961 tcggcgttcc tgctcgagta catcttcatt gccgacgacc gggtctgtat gatcgtctac
     4021 ctcgaggacg ccgtccgcaa cgccgtcgtg atcaccctcc cgttcatcac ctgcgtccag
     4081 aggcagttcg tcaacaggat gatcgcaagc gccgattgca tcagcggccc gggggaagcc
     4141 ggcacagagg cacggccttc cgccacgtaa ccccttgcct gcccctaccc ggagcgggcg
     4201 gagtccagcg gcatgtaggg tgcgaaggtc agaacgtcct ttctgacggc atcgaacccg
     4261 acccgctcga cgaatcgtgc ggtccgttct cccggtttgg cgttctcccg gtagtactcc
     4321 aggagccggc ggaccagatc gagggcctgc tctttcgtga ggtcttttgc ggcgacatcg
     4381 ccgatccgcg gacgcccgcc ggagttgccc ccgaagatga ccgtccagcc tttcgcgttc
     4441 ccgacgatcc cgatatcccg gaggtaactc tcgccgcagc accgggggca cccggagatc
     4501 cccatcttga cctttgccgg caggttcatg tccagataca ggttctcgac ctcgagcccg
     4561 aggctaaggg agtcctgctt gccgtatttg caggtcaccg tccccggaca tgcctgaaca
     4621 tagtggacgc agggcgcggt cgcctgcccg acagtcatcc cgagttcgtt ccatatgcta
     4681 tcgacgtcct cttccttgat gcccacgaac accatccgtt gccctgacgt catcttgatg
     4741 acggggatgt cgtagcgcct gacgaccttg atgatgctct ccaggtgctc tgggctgacg
     4801 attccggcgg gcgttctcgg cacgatcgcg tatgatgtgc cgtctctctg gaggatcgct
     4861 cctctgggtc cgttttccat gctcttgtca cctctccggc agcgtcgggt gcgccggata
     4921 tgattcaccc atcggaatcc ggagggaaaa tcgtgctgtc actctgcgag gtacacccgc
     4981 tcagggatcc ccgtacggcc cttcgctccg cgagcggaag cgccagagca gtacgattac
     5041 tgccaggagc acgatgttcg cggcgagcag gggcaggttt tcggggttcc ccgcgtaggc
     5101 gaggaggccg accgggaaga gtgttgcgga gatgacggcg gtcagcacgg aggcggcaag
     5161 gaatatcggg aaggtctgct cgtagtgtaa acggcgggat acttttcccc agaagtggcg
     5221 gaataatttt ccccacctgt cttcataatc ctttccatat catcccggga gcgtatggga
     5281 gaagagcccc tgcttccgcc gctctttcag tcggtaacta tctcctttga tgttgattgt
     5341 ggtgcagtga tggagaatcc gatccaagac tgcagcagcg atgatatgat caccgaagat
     5401 ctgtcgaata gggagaagcc taaactcccg gaaaagttag gcgcattgtt ccaccaatcc
     5461 cgtgataaat ctcttttttt cggaaatatc tctgtaatcc tattagaatc gaaaggccaa
     5521 gcggatcgca gtgcctaaat tttataggca cattacgcct cagtaagccg gacataaaag
     5581 ggagaaagga agcgaataat
//
LOCUS       DAJB01000105           11791 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_90167, whole genome shotgun sequence.
ACCESSION   DAJB01000105 DAJB01000000
VERSION     DAJB01000105.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_90167"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2073 a         3771 c         3705 g         2240 t
ORIGIN      
        1 gcttttgggg cgggcaaacc tgaccaggat agaatggcag aacaatcacg aagaagcgac
       61 gagccgaccg caggccgtgg tatgagccag ggggagcaag tcctgaaaga gaggaatcct
      121 acccggatta ctgcagagcc cggaaagcag gagatgatcg tcacccggga gttcgacgct
      181 ccacgagacc tgatcttccg ggctcacacc gacccgaaac tctatgagca gtggataggt
      241 ccgcgaggct tcaccacgaa gattgagacg ttcgaatccc gcaacggggg cagctggcgg
      301 tatattcaga cggataagga tggcaacgag tacgggttcc acggggtgaa ccacgatgtg
      361 gcacctcccg agcggatcat ccagacattt gagtacgagg ggctcccgga accgggacac
      421 gtcatcctcg aggcggcgaa gtttgacgag ctgcccggga gccggacaag atttattggc
      481 cggtcggtct tcctgtcggt ggaggatcgc gacgggatga tgcagtccga tatggaggag
      541 ggcatgaagg actcctactc ccggctggac gagctcctgg agagactaca gaagtgaact
      601 accccgccct tacggacggg gaaccgtgcc gtggtgaggg acgacaccgg tgataaatgc
      661 tcgtgtgaaa cagggatccg atctcgacgt cggtagtggg cggtagtaat cgtgagcggt
      721 cgtggcggga ctgccgggtg actgcacgcc ctgtcatccg gccccgggct ggaggatggg
      781 aaggggtgag taaaaactgt ggaacaagcg cgcgtggacg gcgggcgcaa cccccggtgg
      841 aaacacagga gtccggagat catgccccgg ggaaggcacc cttgtccgta tggtgacagc
      901 gcaaaatnna taaccagcta gaccacgagc ccgacgatca ataaagttgg tgctccgggt
      961 ataaaattat tgccacgcgc caaaaaccgc tccgtgccgg ttccgcccca tcgggagtgg
     1021 cacgcgctgc aaaagagggg gttggccgga ccgatcagat ctcggcgtgc ttcgccgtgt
     1081 ggatgccgtc gatcctgcgg atctcggcca tcgcatcggc cggtaccgcc gaatcgaccg
     1141 tcaggaccat cagcgcctct tcgccgggct tgtggcgtcc cacctgcatc ccggcgatgt
     1201 tcacgttcac ccggccgagg atcgtcgcgg ccttgccgat gacgcccggc ttgtcggtgt
     1261 ggcgggagat gaccacgtgg ccggtcgggg tcaggtcggt catgtatccg ccgatgctca
     1321 cgatccgcgc ccggttcggg cctgagacgc tgccgctcac cgactcggtc atcctgtcgg
     1381 tcttcgcggt gatgctgatg atgttccgga atccctgcgc ttcctcagtc gtcgtctcgc
     1441 tcatccggat accgcgctcc tttgcgacga actccgcgtt cacgatgttg accggcacct
     1501 gcaggatcgg gtcgaggagg cccttcaaga tgacgcgggt gacgaacttc gtgttcggca
     1561 gcgccgcggc ctcgcccccg taggtgatct cgatcgactc gagcctgcct tcgatgagct
     1621 ggatgagaag gctgcccatc ttctgcgcga gcaccgcgta gggctcgacc agcgcctgct
     1681 gctctgcggg gatcatcggc gcgttcacca catacttcgc cgggccgccc tcgagcacgc
     1741 tgatgcactg gtttgccacc gagatggaga cgttcttctg cgcctcgacc gtgcttgccc
     1801 cgaggtgcgg cgtcacgatg accgtatcca gcccgaggag cggggagtcc gtcggcggct
     1861 ccgtctcaaa gacatcaagc gccgcaccgg cgaccttgcc gctcgcaatc gcgtccgcga
     1921 gcgccttctc atcgatgatc ccgccacggg cgcagttgat catccgcacg ccgtccttca
     1981 tggtggcgat agacttctcg ttgatgacgt ggcgcgtctc cttgatcagc ggcgtgtgga
     2041 cggtgatgac gtctgccctc cggaagagtt cgtcgagcgg caccagttcc acgccgaggt
     2101 ttgcagccct ctccttgctg atgaacgggt cgtaggcgat gcacttcatc tgcattgccc
     2161 ttgcacgctt cgagacctcg attcctatac gcccgaatcc cacgataccg aggatcttgt
     2221 cgttcaattc gacgcccatg aacttagagc gcttccactc gcccttcttc aaggatgcgg
     2281 tcgcctgggg aatattgcgc gcgagcgaga gcatcatcgc catcgtgtgc tccgtggccg
     2341 cgagagtatt gccttcagga gcgttcgcga cgatgatgcc cctgcgcgtc gccgcctcgg
     2401 tatcgatatt gtcgacgccg gcgccggcac ggccgatgaa cttgagtttc gttcccgcat
     2461 cgatgacccg cgccgtaacc tcggtgcccg agcgtaccag cagggcatcg tagtcgccga
     2521 tagtagcaac gagttgatcc tcggttagcc cggtgttgac atccacatcg cagaatccct
     2581 tcaggatgtc aatcccttcc tctgccagcg ggtcgctgac cagcactcta tatttcacag
     2641 ttcaacccat atctagttcg acatcatatg tcttgatgct ttttcctccg aaatctttta
     2701 tgtaatggtg agaagtatat gtgtggtgcg agcatgaagg aaatgcccaa cgttctgtgg
     2761 ctcgaagaaa taaagaagga agatatcgtc tccgtagggg gaaagggagc atctcttggt
     2821 gagatgacct ccatcggact tcctgtaccg aaggcttttg tggtgactgc ccaggcgttc
     2881 cgcaggttcc tcgttgagac cgggatcgaa gatgcactct tccgcagact ggagcgtctg
     2941 gacgttgaag ataacggagc cctggaatcg gtctccaggg atgtgcagga cctcgtcctg
     3001 agcgtcgaga tccccgatca catcatagag gagatcaccg atgcatacgc ccgtatgggg
     3061 gcggacggaa cggtcgtcgc cgtccggtcg agcgcgaccg ccgaagacct gcctgaggcc
     3121 agtttcgccg gccagcagga gacatacctc aatattctcg gtgatgttga cctcctcgat
     3181 gcggttcagc gatgctgggc ctcgctgtat ggcgcgcgtg ccatctacta ccgggcaaaa
     3241 caggggttcg acgaccggag cgtgaacatc gccgtcgtcg tgcaggaact catccggtcg
     3301 gagaagtccg gggttatgtt cacctcccac cccgttaccg gcgagcccct gacgatcatc
     3361 gagggctcgt ggggactcgg cgaagccatc gtctcgggca gcgtctcccc tgacaactat
     3421 gtctttgacc tgcgctccga gcgggtggtc gaccgcctga tcgccgaaaa ggagatcatg
     3481 atcgtcccgg agggcgagca cgggacgaag atcgtcgctc tcaccggcga acagcggatc
     3541 gccccggtcc tctccgacgc ggaagtggcg cgcctcgcca tgctcgggaa gatcgcagag
     3601 gaccactaca gcaatcctca ggatatcgag tgggcgatcg tcgggagcga cgtcttcatc
     3661 ctccagtccc ggcccataac gaccatccgg cgcccggaga tcccccgtgc cggagcggaa
     3721 tctgcaggaa agggcagggg cacgtttggc gtgatgctgc tcgagggcca gggtgcttcc
     3781 cccggcgtcg cgagcggccg ggttgtgatt gtgcgggacg tcaaggacac gagcgccgtc
     3841 aaggacggcg atatcctggt caccaagatg accaaccccg acatggttcc ggcgatgcgg
     3901 cgggtcagcg ccatcgtcac cgatgaaggc ggcatgacct gccacgcggc catcgtgagc
     3961 cgggaactcg ggacacccgc ggttgtcgga accaagaaag caacgaagat tctcaaagac
     4021 ggacagatca tcaccgtcga cggggagaag ggcacgatct acgagggagt ggtcgaggct
     4081 ccggcggcag caccggcagc cccgacggcg gcggcggcgc ccgctcaggt catcaccggc
     4141 acgctcgtca aggtgaacgt ctccctcccc gaggccgcac agcgggctgc cgcgaccgga
     4201 gccgacggcg tcggcctcct ccggatcgag cacctcatcc tcggcctcgc gaagactccc
     4261 ggctggtata tcgaccacgg cgaggaggag gagttcatcg gcgaactcta cggcggcatc
     4321 aagacggtgc tcgacgcctt ccccggtaag cccgtctggg tcaggaccct ggatgccccg
     4381 accgacgagt tccggaacat ggagggcggc caggacgagc cgatcgagca caacccgatg
     4441 ctcggctggc gcggcatccg ccgcgacctc cggagccccg accagttccg catgcaggtg
     4501 gaggccttca agaggctctg ggctgcaggc tatgacaatc tcggcgtgat gtttcccatg
     4561 gttaaccacc ccagggagtt cgtccaggcc cgggcaatga tgaagggctg gggggtcgac
     4621 gtggagaacg tgaccctcgg catcatggtc gagatcccga gcagcgccat cctcatcgag
     4681 gacttcatca aggccgggat ccggttcgcc tcgttcggga caaacgacct catccagtac
     4741 acgctcgcca tcgaccggaa caacgagaac gtcgccgaca tgtacgagcc cgagcacccc
     4801 gccgtgctcc ggctgatcga ctacgcgatc aaagcctgcc gcgagcacgg tatcgagtgc
     4861 tccatctgcg gccaggccgg ctccgatccc aaaatggctg cgtggcttgt cgggcacggc
     4921 atcaccagcg tgtccgcaaa catcgatgcg attccccgta tccgtgaggc cgtagcacgg
     4981 aaagagcagc agattctcct tgatgcggcg agaagaaatg cgtgaagttg gatgccctga
     5041 ggaggacctc ttctccttcc tcctgtcgag atggcaggag gatctcgggt accgcaatat
     5101 tttaagttcg atgtgcacgc cgccccatcc ggtcgcggcc cgggcgcatg cgatgttcct
     5161 tgagaccaac ctcggcgacc ccgggctctt ccccgggacg gccgcgcttg aggagctcct
     5221 cgtcgagagg ctgggtgcgc tgatgcacca tccgaaagcc ggggggtatg caacaagcgg
     5281 gggaaccgag tctaacatcc aggcgctccg gattgcaaag aaactgagac cgacaacctc
     5341 cccgaacgtg gtcgtcccgg cgtcgagcca cttctcgttc cagaaggcct gcgacatcct
     5401 gggcctcgag atgcgggcgg caccgctcga cgcccggttc cggatggaca cggaggccct
     5461 cgacggtctc gtcgacagga acaccgtctg cctcgtcggc atcgtcggga caacggagta
     5521 cgggatggtc gaccccattg ccgctctctc cgacgttgcc gaggacaggg acaccttcct
     5581 tcacgtcgac gctgcgttcg gcggcatggt ggtcccgttc cttgaccggc cggtcccgtt
     5641 cgacttccgc ctccccggtg tcgcatccat ctctgtggac ccccacaaga tggggatgag
     5701 caccatcccg gcaggctgcc tcctgacccg ggacccgtcg tgcttctcgc acctcaacgt
     5761 cgagactccg tacctgacgg tgaagcggga gtgcaccctc gccggcaccc gcccgggcgc
     5821 atcggtggcc gctgcctttg cggtcctcga atacctgggg atggacggca tgcgggcggt
     5881 ggttgccggg tgcatggaga acactcgaag gctgatcgag gggatggaga cgctcgggta
     5941 cccgagagcg gtgacaccgg acgtcaacgt ggcgacgttc tcctgtgttc gcgtacctgc
     6001 cggctggcgg gtctcgacga cccggaacgg acatatgcgg atcgtctgca tgcctcacgt
     6061 cacccgcgac gtgatcgagg catttctcaa agatatgagt gatacgaatg cttgaacgtc
     6121 tgatacattc tctggagacc tccccggtca tgaagcgggg agagtacaac tactttatca
     6181 acccgatcac cgacggggtg ccgctccttg agcccgcggt cctccgcgag atcgggtgcg
     6241 ccatggtgcg gtgcctcgac ctcgaaggcg tcgacaagat cgtcgtctgc gaggcgatgg
     6301 gcatccacat cggtgtcgcg ctctcgatga tgaccgacat cccgctggta gtcgtcagga
     6361 agcgctccta cgacctcccc ggcgaggtcg cggtgcacca gaccacgggc tactcgaagg
     6421 gagaactcta cctgaacggc gtcggtgcgg gcgaccgggt cgtgatcatc gacgacgtct
     6481 gcagcaccgg ggggacgctc cgcgctctca tcagcgctat cgagcaggtg ggcgccgaga
     6541 ttgccgacat ctgcgtggtc atcggccggg gcgacgcgga tatctgccgc cccctcaaga
     6601 ccctggtcaa gatcgaggtc tccgagacgc aggtgcgtgt cgttgatacc tgtctctgat
     6661 cttatcggga ggctccgcga gcgcggggcc cggagcgtcg ccctccagtt ccctgccggg
     6721 cttgcgcggc aggctccggg gacggccgct gccctccgcc gcgagggttt cgatgtgatc
     6781 gtcagcggcg acccctgcta cggcgcctgc gacctcgcgc tcgatgccct cgcatacgcc
     6841 gacgtcctgg tccacttcgg ccacgcgccg gtcgaggagc ggccggacgt actctacgag
     6901 cccgtccgct tcgacttcga cgtgggcgtg ctcgagaacg ttctgccgct gctggcgggt
     6961 cgccgcatcg gcctcgtcac cacggtccag cacgttcacc tgatcgatgc gatgacggcg
     7021 ttcctccgtg agcacggcat cgaggcactc gccgctcccg gcgacggtcg cgcaccgctc
     7081 gtcgggcagg tgctcgggtg caacttcacc gcagcacggg cgacgggagc cgacgagatc
     7141 ctcttcgtcg ggaccggggt cttccacccg gtcggcatcc agctcgccac cggggcgcgg
     7201 gtggtggcgc tcgacccgtt cacgggcgag gtgcaggagg tggacgcctc ccgcctggtc
     7261 cgccgccgcg ccgcggtgat ggagaaggcc cgggatgccg caagtttcgg catcatcgtc
     7321 agcacaaaga gcgggcagca gcggatgagc cttgcccggc ggctcgtcgc cctctcggat
     7381 cgggccttcc tcgtcgcgat gcgcgaggtc tcgcctgctg agatgctcga cctcgggttt
     7441 ggcgcctacg tgaacaccgc ctgcccccgg ctcgcctacg acgaccaggt ccggttcccc
     7501 gtcccggtgc tgacgccgcc ggagttcgag atcctctgcg gggcccgcgc ctgggacgac
     7561 tacgcaatcg acgagtacct ctccccatga atcttcgaca gcttgagatg cggctggaac
     7621 gcctggaggg ttatgagcgg ccgatagcgc gcctcgagca gtaccagacc ccggcgccgg
     7681 tggcggcccg cctcctccac catgccgcca tgcagggggc gatcgagggg cggcgggtct
     7741 gcgacctcgg gtgcgggacg gggatcctcg cctgcggcgc cgccctcctc ggcgcctcca
     7801 ccgtgaccgg tctggatatc gacccggccg ccatcagcgt tgcccggcgg aatgcagaga
     7861 tgctcggtgt ctccgtcgat ttcatcgtcg ccgatatccg gagccccgat gtcgaccggg
     7921 cgggcctggc ctgcgacacc gtcgtgatga accccccgtt cggggcgcag aaggcgcacg
     7981 ccgaccggcc gttcatcgac cgggcgctcg aactcgcggc cgaggtctac ggcatcttca
     8041 acgagggctc agccccgttc gtggccacct ataccgaagg ccgggcgacg gtcgaggagg
     8101 tgatccggtg cgcgttcctg ctccggaaga cgtttgccca ccaccgcaaa gaacgagtgg
     8161 atatcacggt ggaagtcata cggttaagac ggatctgaca tgcgtgactg ctgatacaaa
     8221 atcggtttgg gggctctctg cggcccacct ggtgaccgac ctctactctc cggtcctccc
     8281 tgcaatcctg cccctcctca tcgcggagca gggctactcg ttcttccttg caggactgat
     8341 cgtcacggtc tacaacctca cgtcgtcgtt cgtgcagccg ctcgtcggct ggctcttcga
     8401 tacgaagggg ttcgccgtcc acatcagcac gagcgtcctt atcagcgcga tattcatctc
     8461 cgtgatcggc ctcctggaca actactacgt cttactcgca tccgtcgccg tcgcggcgct
     8521 ggggcacgcc ttcttccacc cgagtgcgct cgggaccgtc agccgcctga cacaggatgc
     8581 aagccggggc cggatcacct cctacttcgt catcggcggc aacctgggct acgcgatcgg
     8641 tccgatctgc gcgggtgtcg cggtcggcct catgggtctt ccgggcctcg tcatcctggt
     8701 catccccggc atcgcgatgg cggcggtgct ccggcgtatc ctgcccgccc ccgagcgcct
     8761 taaggttccg gcagcacccg cccggaagga gcggcccgcg taccgggcga tcgccctcct
     8821 ggtcgtagcc tcgggcctcc gggcatgggc gatattcggc tcggtcgctt acctccccac
     8881 catgctcacc ctgcggggca tcgacctcgt gaccgcgaac gtgctggtct cgggcatgct
     8941 catcgccggg gtcgtggggc agctcgtcgg cggtatgctc tccgaccgct atggccgcaa
     9001 agaatacacg atcatcgggc ttgttactgc catcccgccg ttcatcatct tccttacaac
     9061 cggtggtatt ctctcgctgg ccgcgctgat ggtctttggg ttcatcctct ggtcgacctt
     9121 cgccgtcacc gtcgccatgg cccacgagat ggccccgggg aacgtcgggc tcgtctccgg
     9181 cctgatgctc gggctcgcgg tcggcgtggg cgggatgggg gttgcggcaa ccgggtgggt
     9241 cgccgacctg acggcctcgc tctcgctcgg tctcatgacg atcccgctcg cgatcgccct
     9301 tgctatcccg ctcttcctcg ccgtccccta cccctggaag tccctcgtgc ggagccgggt
     9361 ttcccgggga tgatcctgcc ccatcccctt aagtaccggg agtgcagatt catccccgat
     9421 ggtcacccgg ttccagcgct accggcagat cgcggatgtg ctggtcaagt acggttttgg
     9481 gatcctcgtc gaagaggtca ttcccggtgg cgagcggctc agggggttcc ggaaaccccc
     9541 tgaagagaag cgatcggtct acgaacgtat ccggcttgcc atcgaggagc tggggcctac
     9601 ctacgtaaag ttcggccaga tcatgagcac ccgccgggag ctccttccgc ccgagctgat
     9661 cgtggaactg cagatgctgc aggaccgggt tgcgcctgtc ccgtttaggg atatccggcc
     9721 ggtgatcaag aagtattgcc cgaacctcga ggactgtttc gatatcatcg aagaggagcc
     9781 ggtcgcagcg gcctccctct cgcaggtgca tcgcgcggtg acgaaagaag gccgcatcat
     9841 cgccctcaag gtgcagcgcc cgggaatcgt cgacctgatc gagaccgacc tcctgatcct
     9901 gcagtcgctc gtgacacgaa tggattcgat cttcccggac ctgcgggtat acaacctgcg
     9961 ggggatggtg gacgagttct cggcccagat ccggcgcgaa ctggacttca cccaggacgg
    10021 gatgaacgcg gagcgcctcg cacggaatat gcacgacgtc ccgtgcgtga agatccccgg
    10081 tatccactgg aacctctccg gcccctgcct gcttgccatg gactatgtcg agggggtgcg
    10141 gatcgacgat gtggaggcga tccgggctct tggtctcttt ccggaagagg tcgccgacat
    10201 cggtttttcc gcctatgtcc agcagatctt cgtcgacggg ttctttcacg gcgaccccca
    10261 cccgggcaac ctcctggtga cggatcaggg cgagatcgcc tttctcgact acggcatcgt
    10321 cggcgtcctc cgcccggaga ggcgccgcgt ctttgtcgat ctcctcctcg ccatgacccg
    10381 cacagacgtc gtcggcgtta tcacggcact cgggaggctc gaggtgcata tcagcccggc
    10441 ggacctcgat gccgtgaagg atgatctcta cctggtcctg ctggattacc gggagatgaa
    10501 actcgaacgg gtgaactttg cggtcgcgat ccggggcctg accgataccc ttcgccggta
    10561 tcacatccgg gtcccgtcta ccctgatgct gatgatgaag gtgatcgtca tggtgatgga
    10621 catcggcacc cggctcgacc cgcaattcaa cttcgatcag cagatccggc cctacctgat
    10681 agagatcgcc gcacagcagc ggttatcccc ggataacgtg acgggtgcgg tgcggtctct
    10741 cgtgaacgcc gcggagggcc tgcttgccat cccgggcaac gtaaacgaga ccttaaagac
    10801 cctctcggag gggacggtca cgatcgagct cgaaaaccgc gacctcaacg agatcgtcga
    10861 cgtcatcgac cggacgagcg acaagatcat cgtcgggctg gtggtcgccg cgatcgtcgt
    10921 tggctcgtcg ctgatactcc ggatagcgga tctcccggtc cccggatacg tcacgattct
    10981 cgcaaccgcg ggctacgtgg ttgcggtgat cgtcgggttc tatgcagtct acagcgccct
    11041 caggtatagc cgtgggttga ggaggtgaac gacgctccat caggagcgga gttcgagcac
    11101 cgtaaggtgc gagtgacgac gggcggtggc cggccctctc ccgggagccg ggaggacaac
    11161 cgtcaaaaaa gaagttattc gggtttcttg aagaacagcc tgcagtggca ggtatcctgc
    11221 tcctcgatct ctttctcgtg gtagatgcag gggcagacga tgatccggtc tttctcggga
    11281 tccccgctcc ggagcctgca ggggcagtat gcctcgccga accgttcttt gttccgggcg
    11341 agcccgcgga ggatggtcga gagctgtttc tcgtcgacgt ttaagatgta gcccttctcc
    11401 ttcgcgtacg cctttgcccc ggtacgtgcc tcttcgatcc cttcttcggt catgataaaa
    11461 ctcctatcgt gtggttccct ggctgctgta gagccaccta caggtatcga gcgcaccgtt
    11521 cagccgtgtc tcagtggttg catcgggata cgcccggtac ttgtaggaaa cgatcattta
    11581 cttccctatc tgcgagtcaa catactcctt cagcgcatcg aggcttacct gtcctgacgt
    11641 acctgtaccg aatgtgcaga cctatcaagt ttatacctca tgttgggtga atgataatag
    11701 aatactcaag aggaatatat ctatcgcagg aaggaagggc ggctccgctt tcatcccctc
    11761 tcctctcgac cggggacttc ccgctccgcc c
//
LOCUS       DAJB01000106            6458 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_11244, whole genome shotgun sequence.
ACCESSION   DAJB01000106 DAJB01000000
VERSION     DAJB01000106.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_11244"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1045 a         1968 c         2135 g         1310 t
ORIGIN      
        1 tccgggaagt agtccagcag gcagtaaggc tgctccccgg ccttccttcc gtcgaagtgg
       61 cgggagtagt tctcgatccc cttgcaggtc ccggtctccc ggatcatctc gatgtcgtag
      121 agcgtcctct gccgcaggcg gtgcgcctcg agcatgccga ggttcgggag ccactcctcg
      181 agttcctgct cgatcgaggt tatcgcccgc tctttctcct cctccgggac gacgaagtgg
      241 cgggccgggt agacgaagaa gtagttcatc tcttcaaggc gctggccgga gaccttatcg
      301 atctcgctga tccggtccac ctcgtccccg aagagctcga tacggatgat gttgttgaag
      361 taaccgggga caaggtcgat ggtgtcgccg cgcgcccgga accggcccgg cgcgagttcg
      421 atgtcgtttc ggtcgaactg gatgtcgacg agcctccgta taatatcgtc ccgccgcatc
      481 cggtcccgga ccttcacctc aaaccccatc cgctgaaagt tggcggggtt tccgaggccg
      541 tagatgcagg agaccgaggc gacgacgatg gtgtcggggc gcgagaggac cgatgcagtg
      601 gtggcgaggc gcatctgctc gatcttcggg ttgatctggg cgtctttttc gatgtagagg
      661 tcgcgcttcg cgatgtagga ctcgggctgg tagtagtcgt agtaggagac gaagtactcc
      721 acccggttct cggggaagaa cgatttgaat tcgttgtaga gctgggccgc aagcgtcttg
      781 ttgtgggcga tgacgagcgt gggtctctgg accgcctcga cgacgtttgc tatcgtgaac
      841 gtcttgcccg agcccgtgac cccgaggagg gtctggaacc gctcgccccg ggagagcccg
      901 ccggtgagtt tttggatagc gtcgggctgc gagcccgtgg gtttaaaatc cgccttgagc
      961 tgaaatgccg tcatggtgcc accgtcttct tccggagggt cctgtggtag gcgatcgcga
     1021 accggtgcgc ctcgtcgcgg atctcctgga ggaagagcga cgctttctcg tgcctctcaa
     1081 gcggcagggg ctgggagaag cccggcacga agacttcctc ctcgcgcttc gcgatggccg
     1141 cgaccgggac tttgatcccg agttccctga gcaccgcaac ggccgctgcg agctgcccct
     1201 tcccgccgtc gacgaggatc aggtcgggga gttcgccgcc ctccttctgc aggcgcgcgt
     1261 agcgccggcg gaccacctcg gcgatggcgg cgaagtcgtc gacgccctcg accgttcgta
     1321 tcgcaaagcg ccggtagttc cgcttgtcgg gctttcccca gaggaaccgg accatcgacc
     1381 ccaccatcgc cgtcccggag agatgggaga tgtcgaagca ctcgatcgcg accgggaggt
     1441 ccgggaggtg caggcggcgt ttcagttcgt ccagtttgat ccggtcgccg aagaacgcga
     1501 tctccacgtt cttccggaca aggtcgagca ggttcttctt ctcgccccgc tgcggcacga
     1561 ccacccggac cctgccgccc cggaggtggg agaggtagcc ggcgacggcg tcgtcgaccg
     1621 gcacgggcag gatcacctcc tccgggggtt cgtggtcggc gtagtagcgg gcgaggaact
     1681 cctccaggaa ctcctccgtc tcgtcgaagg tgtactcgtg cttcccggca agcgttcctt
     1741 tgtagacgtt gaagagcatg agggagacgg ttccatcgct tgcgatatag ttgacgatat
     1801 cctcgttgta ggtcttctgc cggtccatgt gctgccgctc gcgcagcccc tcgagggccg
     1861 cgacctggtc gcggagctcc atcgcccgct cgtactcctg ccgctctgca gaagccgcca
     1921 tctcctcgcg gagcgcccgg atgagcccgg cgatatcccc tttgaggacc gcttcggctc
     1981 tcctgatccg ctcccggtag tcgtcctcgc cgattgctcc ggtgcagggg gcgctgcacg
     2041 agccgatgtg tgcacggagg catggacgcc gggggaggcg gcggcaggac cggaggcaaa
     2101 agagggattt taagaggcgg aggaggtctt cgcgctcccg gccggagacg aacggcccgt
     2161 agtaccggcc gttcccgtcg ggttggcggg cagtgtggat ccgggggtag ggctcgtcgg
     2221 tgacgtggat gtaggcgtac ctcttcgcgt cctttaagtc gatgttgtac ttcggctggt
     2281 gcttcttgat cagggtgttc tcgaggatga acgcctcgac ctcggtgtcg gtgacgatga
     2341 aatcgatccc ggcgatcgcg gcgacgaggt tttcggtctt ccggtcgagg tcgtgccggg
     2401 agaagtaact cgatacgcgc ttcttaaggt tctttgcctt cccgacgtag atgatggtgc
     2461 cctcgtcgtc ggagaagagg tagcacccgg gctcggtcgg taaggttagc gggtcgatca
     2521 tggcttttta aggatctctt tgaggaatgc tccggtataa ctcccttccg ccgccgccac
     2581 ctcttcgggg gttcccgccg cgacgatctc accaccggcg tccccgcctt cggggccgag
     2641 gtcgatgacg tagtcggccg acttgatcac gtccaggttg tgctcgatca ccgccatcgt
     2701 gttcccccgg gccaccaggt cgtcgaggac ggcgatcagg ttcttcacgt cgtggaagtg
     2761 cagcccggtc gtcggttcgt cgaggaggta gatcgtcttt cccgtagccc tttttgcgag
     2821 ttcccgggtc agtttgatcc gctgcgcctc gccgcccgag agggtggtgg agctctggcc
     2881 gagtttgatg tagccgagcc ccacgcggca gagggtatcg agtttgttcc tgatcgaggg
     2941 gacgttctca aagaggttca tcgcttcctc gacgctcatg tcgaggacgt cggcgatgga
     3001 cctgccccgg tacttcacct cgagggtctc gcggttgtag cgtgccccct tgcactcctc
     3061 gcactcgacg tagacgtcgg ggaggaagtt catctcgatc ttgatgagcc cttcgccctg
     3121 gcaggcctcg catcgccctc ctttgacgtt gaacgagaac cggccgggct tgtagccccg
     3181 caccttcgcc tccttcgtct cggcaaagat cttccggatc tcgtcgaata ccttggtgta
     3241 ggtcgccggg ttcgagcgcg gcgtcctccc gatggggctc tggtcgatga cgatcacctt
     3301 gtcgatctcg tcgtcgaaga cgaggctgtc gtactccccc gggcactcac gcgagtcgtg
     3361 gagtttctgc atcagcgccc gatagagcgt ctcgtagatg agcgtggact tccccgaccc
     3421 cgatactccg gtgacgaccg tcaggacgcc gatggggatc tccacgtcga tgtcttttaa
     3481 gttgttcgcc cgacagccgg agagccggat gaaccggtcg ctctgtcggc ggacggcggg
     3541 cacgtctatc ctgaggtcgc cggaaaggta gcgccccgtg agcgatgccg ggtttgcggc
     3601 gacggcttcg ggcgtccctt ccgcaacgat atcgccgccg tggagcccgg cgccggggcc
     3661 catatccacg acccagtcgg cgctccggat cgtctcctcg tcgtgctcca ccacgacgag
     3721 ggtgttgccg aggtcgcgga ggtgctgcag ggtctcgagg agttttctgt tgtcccgctg
     3781 gtgcagcccg atggagggct cgtcgaggac gtagagcacg ccggtgaggt tcgacccgat
     3841 ctgcgtcgcg agacgtatcc gctgtgcttc cccgcccgag aggctcccgg cgctccgcga
     3901 gagggtcagg tagccgacgc cgacctgctc aaggaactcg agccgggcga cgatctcctt
     3961 taagacctgc tttgcgatct cgtgctcgct ctcggtgagt tccagggtcc ggaagaacga
     4021 aagcgctccc gtaacagaga ggtcggtgac ctcgacgatg gatttcccgg caaccttcac
     4081 ggcaaggacc ttctctttga gccgtttgcc cccgcacttc gggcagggca ggatccgcat
     4141 gaaccgctcg aggtcgcggc ggcggtactc ggactgggtc tggtggtaga gccgctcggc
     4201 ctgcggggcg agcccctccc actgtccgga atgcgaccag gaggcgtcgc cgttctttgc
     4261 gttcatggag aaccggagcc ggtcggtcga gccgtacatg agggccttat actggtgctc
     4321 ggtgaggtcc ttgatcgggg ttaagaccga gaacccgaag tgctcggcga cccccgccag
     4381 gtagtgcgcc cgataaccgt cgaggtaggt ccggtagagc atgacggcgc cgtcggcgat
     4441 gcacttctct ttgtccggga cgatcaggtc ggggtcgaac tccatcctga tcccgaggcc
     4501 gttgcactcc tcgcaggcgc cgaacgggct gttgaaggag aacatccggg gctggagctc
     4561 ctcgaacgca attccgcaga caggacaggc catgagcgac gagcaggtct cttcgcggcc
     4621 ggactcatcg acggcgatga caagccctcc tgatttctta agggcgttct cgatggcttc
     4681 gacgagcctc gatcgttcgt cgacgctcaa tcggtcgatg accacgtcga tgtcgtgctt
     4741 ccggtagcgt tcgaggacga tctcctcgtc ggtccggtgg atctcgccgt tgacccggac
     4801 ccgggtgtaa ccctcccggt cgaggtcctt gaagacctgc tggtaggtcc ctttcttctg
     4861 ccgcacgacc ggggcgagga tggtgaccgt cccggccagg gtggaggcta tgcggtcggc
     4921 gatcttctcc ggcgactggg cctcgatggg gatatggtgc tccgggcaga acggcgtccc
     4981 tacccgggcg aagaggagcc gcaggtagtc gtagatctcg gtgaccgtgc cgacggtgct
     5041 ccgggggttt ttggacgttg tcttctgctc gatggagatg gcaggcgaga ggccgtcgat
     5101 gctgtcgacg tccggcttct tcatcagccc gaggaactgc cgcgcgtagg cggagagcga
     5161 ctccacgtag cgccgctgcc cctcggcgta gatggtgtcg aaggcaagcg tggatttccc
     5221 ggatccggac acgccggtga ggacgatgag ccgatcccgc gggagttcga cggtgatgtc
     5281 gttgaggttg tgctcccgcg cccccttgat gacgatattc ttcattggtg cgtgcagata
     5341 tgttggtctt cctacctcat agccgtttgc cgatcccaaa ttttaaatag gtgtttggaa
     5401 cggagcccgg aacgcagccg atctggaggg ctccggctgc gggtggcggg accggggaga
     5461 agaatgccat ccgggcctcc ggcaccgaga gccggactgt ctttcacggg tgcgatcgtc
     5521 ccgccagacc ctctgcaggg tcaggctttt tgcttttccc ggtccaaccg atatacacga
     5581 aaaaggaact ctttttcatg caggaggatc ggttagtgca gagtgcgggt gccccccggc
     5641 gggtcgtcga ggtcgtgcag gacaggcacg ggaaccggat tgagatattc gaccccccgt
     5701 tctaccgtaa ggagttcgag gacgcctacc ggttcatcat cgacgattat gcggttgcac
     5761 aaaaggagat cggcctcttt ctgccctgcg ccgtgcggaa accctacagt cagagcccga
     5821 gccaccagct cttcaggcgg atcatcgacg gcgtccttga cccggaggag taccatatcg
     5881 tcatattcgg cacctgcggg accgtccctg cggaactcga gtgcatgtac ccctaccaaa
     5941 actaccacta catgctcggc aagaccacgg acgggcgggt ccggcaggac ttccaccgga
     6001 tcgaggtcta ccggctgaag ggctaccttg agaagacccg cgacacctac cggcaccggc
     6061 tcgcctactg catcgggccg tttcggaagg caatggttga ggcgtcggag gagaccggga
     6121 tcgccgtcga cctgctgcct tccgacccga tgatcgagcg gctctacgac atcgactgcc
     6181 cgttccccga aggcagcctc tcgatgcagg gctacatcga cgagttccgg gcggggctcg
     6241 cgaggctggc ggggatggtg cacgccgggg gcacgacggc tctggaaggg tgcccggagc
     6301 ggcgggggcc ggtgagggcg tgaggggtgg tggagaacca tacaaagaag agttggagac
     6361 aggggagggg gctcgccccc tcaaagccta acggcttctc gtgctccgtt cctgtcggaa
     6421 catcgcactc cccgtcagcc cctccccaat ggcgatat
//
LOCUS       DAJB01000107            7846 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_34005, whole genome shotgun sequence.
ACCESSION   DAJB01000107 DAJB01000000
VERSION     DAJB01000107.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_34005"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1443 a         2444 c         2471 g         1488 t
ORIGIN      
        1 gcccggcctc cggcctcctc ccccgcccca gagcgggggc agtcatggcg atacccggtg
       61 gaaagccgtg cccctgagta cccctgatta tggccatctg gagcaaacaa gtgggggttc
      121 aacaaagccc taaaaacact tttcatcgat cccccgatgg gatcttctcg ccccgggtca
      181 ggggattttc ccggcgtgag tatgacgaac ggtgcgacct gcccttcgcc cggaaattca
      241 ctccccggcc ctcccccacg gagcacaact cccgcgaggg gtcgttgacg ccccggcggc
      301 atcccgcccg ttccgcgagt tttatccgga caggagacaa cagggcgtgt ggatgaggtg
      361 tccatgatgt ttcgtacgac aatccgggta gatacccacg gggaggggga ggtcgtcaac
      421 ctcacgcccc gggtagcaca ggcggtcgcc gagagcggcg tgcgggaggg gcttgcaagc
      481 gtcttcgtcg cagggtcgac cgccgccgtc accaccatcg agtacgagcc cggtgtgctc
      541 tcggaccttt cccgggcgct ctcggtcatc gccccggccg atatcccgta cgcgcacgat
      601 gcggcatggg gcgacggcaa cgggcggtct cacgtcaggg cggcgatcgt cggcccgtcg
      661 ctctccgtcc cggtgatcgc cggtgcgctc gcgtgcggca cctggcagca gatcgtcctc
      721 ctggaactcg acgtgcggtc gaggcgcgag cggacggtct acgtcaccgt cgtggactaa
      781 aacgcgaggg aggagacgca cggcggcgat actgcgggca gagcgatagg gtcgcctgac
      841 aggcaacccg agaatacgta tcgcactgcc ctggtcttcg ccggaattgt tcaggaagaa
      901 tgagtgattt aatcgagtgg taccgtttcc agctgcgata ccagctccca gatgcgagtc
      961 cgtgcatgaa ccggcatgtt cgggtcgttg ctgatctcgt cgatcatgga gattgcggtt
     1021 gcggcgcgaa gaccaatgct cctggattcg tcctggagga ccgttctggt cgcgtctgcg
     1081 acgcgcctta tattgcgtgg aatggtggaa tcctcgctga tattctgcag catctggatg
     1141 cagatccgta tcgtttcgtc tggacttgcc attggattta cccctctatt attaatggca
     1201 tccagacata tataagttga ttaaggaagt tcaacgaaat gacggctgat caggctgacg
     1261 aagtcatggc cgggtatctc ctgaagggag gaaagatgct cgctaaatcc tgtaaggtct
     1321 gcgggtatcc cctgtttgag tatcagggag agacacagtg cgtgatctgc ccgctcgctg
     1381 cacccggcgg aacgcttccc aaaccggagc aggcggctcc ggcccggcag cccgagcagt
     1441 ctcccgcacc ggcggcggtc gcccgggagc cgcagggccg ggtcaccgag gaactcgagc
     1501 ggacgatcgt ccacctctgc gagcggatca ggagcgagca gagggccggc gaatgcctga
     1561 ccctgatgac ggcggttgag cggggcgcag aagccctcgc aagactgggt cagcggtaag
     1621 gcctgcgggc aagatcccat attccctgcg cggtcttttc tccgatcccg tgcacggatg
     1681 ccagttcctc cgcttcggca tcgatgatcg ccttgagcga cccgaaatgc tcgaggagga
     1741 gccgtgcact cttcataccg atgttcggga acgaagcgag cgcatactcc tgctcttcac
     1801 ggaccgagcg gtaggacttc ttcggatgca ccttgtgctc tccccgctcg ctcccctccc
     1861 gctgtgcaag gatgtagagc atctcggcgg tgtcgtccgc atcgctcgtg cgcatcagcg
     1921 tgacccccat atccaccgtg atagcggcaa gtgtgccccg gacggcgtta gggtggatgt
     1981 tgcgcacttc gtagaggtct tcctcgccct cgatgatcag gacgggacgc ggcaccgcac
     2041 cggccatggc cttgatctgc ccgaagaggt cccgctcgac cagggtgttc atgaagtcct
     2101 ggacggtctt gcgctcgaca aggattcgat cgccgatggc gtagtcgccg aactcgaggc
     2161 gctcgagggt gatcgatgcc ccgagttcgt gcaggcgctc ggcgacccgt gacgacgtct
     2221 cccggtcgtc gacggtgatc gccggtcccg ggggagcgaa cgcgaggaag tctatctgtc
     2281 tggtggcggc cgggataggg gcgggcgcag tgctcatgct cttgatcccc gtcaccatcg
     2341 cccgctcccg ggactggctc acgtaccgga acgtctcgtc tgaggtgcct ttcgtcacca
     2401 gcacgacgat cgtgccgctg ccgctcctcc cggtccgccc cttccgctgg atgctccgga
     2461 tctccgaggg gacggcctcg taaaagatca ccatatccgt cgaggggacg tcgagcccct
     2521 cctcgcccac ggaggtcgcg accagacacc tgaactcacc ctcccggaac cgggcgaggc
     2581 tcgcgatctg ctctttctgc gagagccccc gctccgcatc ccgggaagcc tggccgacga
     2641 accgctcgca ggcgatgccg gccgcggaga gcgcctcgac aagcgtctga acggtgtcgc
     2701 ggtaggtggc aaagacgatg atccggctct ccgggtgcgc cgcgagttgc gcccgcacca
     2761 gttcgcggac gatggcgacc ttgggatgga gttcctcctt ccagccgccg gagacctcga
     2821 cgaggctctg gtaggcaggg tcgccgacga ggcgcttgct cgccttgctc cctgcgctcg
     2881 aggccccttc cgcgccgagc cgggccaggt agagtttgag cgccccgctt ccctgggtct
     2941 cgacgagcaa gatggcatgg cggagtttca tgcactcggc gtgcagggat gccgcgatga
     3001 acgcagaggg gtcccgtgtc tgtatacgct gctggatctg ggcgtttaag gcgttgagcg
     3061 ctttcatcga gagtttatcc gctttcggga cctggaagtt gaggctcgcg agccgggtga
     3121 ggcgcgactc cacgagtccc cggaggacaa cgagcgccgc ctgcagttct tcaggcaggt
     3181 agacgtcgac gtactggatg tcgcgctcat ggatgtaggg ccggacatcc tcgtcggtat
     3241 ccacccttgt ttcgaccgcc tcgatatgca ggttgttgca gacctcctgc accttcacct
     3301 ggtcccctcc gggagaagcg gtcatcgcga gcagcagggg ctccctcgcc gtggtgcagt
     3361 agtgctgcgc taaaaagaca tacgcatagt tccccaccgc ccggtggcac tcgtccacga
     3421 ccagcagcac gacgtcggcg aggctgtacc tccctgccag gcagtcgttc ttgatcacct
     3481 gcggggtggc gaaacagacc cggcacgcct cccaggctct ggccctctcc tcaggggggg
     3541 tgtccccggt gaacatcaca aaatcggact cctgcggctc ggaaccctcc cgggagagca
     3601 tgaattgcct gaaaaaacga agatgctgct cgaccagggg ttttgtgggc gcaagcatca
     3661 gcactcgccc cggatgggag tagaggcggg atgccgcgac gatgagcgcg acggccgtct
     3721 tcccgaggcc cgtcgggagg acgaccatcg tgttcgcgtc aagtgcgcgg agcgcgatgg
     3781 agagttggta ccgccggtcc tctatgctct ctggccggat cagcgggtgg gagatggagg
     3841 tcatgccctg agttcatgga acgtgatggt gccggcgatg agtccggaga gggtctccgg
     3901 cagccgctcg accggcacac ggacctgctc catgctgtca cggtccctga cagtcgcggt
     3961 gttgtcctca agcgtatcgt agtcgacggt gacggcaaag ggtgtcccga cctcgtcctg
     4021 tctccggtaa cgcctgccaa tggcgccgga gtcgtcatac tgggcgagga tgcggcatcc
     4081 ggtgagccgc tcgacgatcg tctgcgcgat cgtatcgagg ccgtcccggt tcatcagcgg
     4141 gaagacggcg acctggatgg gcgcgaccgc cggcgggagc cggagcacct ttcttatctc
     4201 gccctcgacc tcctcctcgg cgtagctgtg ctcgagagcg acgtagatca tccggtcgat
     4261 gccgtacgat ggttcgatga cgtggggcat cacctcttcg ccgcgaacct ccacctcttc
     4321 ctcccggatc tggtagaggt cggcggagat gaagaactcc tcgccgtcga tggtgacccg
     4381 cgcgccgtcc tctccggggc cggaatcggc cagggcatcc gcgatcgcct tcgccttgcc
     4441 gcggaaccgg gggccgagca cgcccatgtc ggcgatgatc cgcttcttcg cgacccgctg
     4501 cggttcgtcg tagggcatga agaccgtcat cgaggtgccg gactgcccgg catgcgagcg
     4561 caggtcgtag ttggtgcggt cggcgatgcc gacgatctcc acccagccga agcggccgga
     4621 gtagacctcg gcgtcccagc agtcggccgc atagtgtgcc cgctcatcgg tcaggtgctg
     4681 gcggaaccgg agtctcgccg ggtccacgcc gatggaggtc aggatccggt gcgtgagcgc
     4741 gatatagtag gcgacgtact cgtttgcaat cagcccttcg tccacggcgg cacgcatcgt
     4801 gatggcggcc ggctcctggc catcgagctg ccgggcgatg gtgaggaggg gcgctgcata
     4861 ctccgcgtag cgctggaagg cgggatggtt cttccggtcg gggtggacga agatctcggc
     4921 ctcagcctgg gagaactccc gcagccggat catgccctgg cggggagaga tctcgttccg
     4981 gtaggacttc ccgatctgga cggcgccgaa ggggagtttg tcccggtaga agcgcaggag
     5041 ccggggaaag tcggtgaaga taccctgggc agtctcggga cgcaggtagc ccttccgttg
     5101 cgaccccggg ccgatggtcg tctcgaacat caaattgaag gcaaagacac ccgcttcgcc
     5161 caggggtttc tcgcatgcgg ggcagggaag gttacagagc gctgcctgga gcgcctccgc
     5221 cgggagcgtg cctgcattct cgatgccgtt ctctgcggca atatgatcgg cgcggagata
     5281 ctcgccgcag tgcggacact gcaccatctt atcggcaaac tctttcacat gaccggatgc
     5341 gatgaagatt gcctcatttc ctacggtggg acattctatc tcgtagtagc cttcctggaa
     5401 gacgtagaag gatcgccaga ggttctcgac gttccgcttc atcatggctc cgagcgggcc
     5461 gtaatcgatg aacccggcaa tcgacccgta tatctcggaa gagggccaga caaaaccacg
     5521 tcttctggct acttccatga ctttttcgta aatatcgctc gtctcggcca tagtcctgca
     5581 gtacatttga gtgccggtgc taataaagaa tggtcatccc ctcgcgcgag atcacaattc
     5641 ttcgaaaaac aggccggatg cggaggtttt ttttccgcag tgggcgatat cccggaagat
     5701 attgtcacat ctgcaaaaac gatctttctg tttcgacggg ttatatggta aactttaaat
     5761 cttagcggtt gtaatgtagg gaacgttacg ccccaatcgg gggataaact atatatccga
     5821 ggtcatcatg ggtgaccccc aggaccagac ggtggaatac tgaacagaga gagactgaca
     5881 tggcagaaga tacccgcaag cagcagctcc ttgagctgtt tacgaccatc acgaacaaga
     5941 agacgatcgt tgagccgatg agaaaggttc acggtacgct ccgggatcgc gacgcagttg
     6001 agcgcgagat cgccctgatc atgcgggaag tcctcgatca ggggtacttc aagaccaaac
     6061 tcgccccacg gcagctctcc agactcgtgg ttgcgtacta cgacggcaag aacgacaccg
     6121 agatcgcaag ggcgctcggt gatgagaagt tgagcaagac cgtggcacgt gcccgggtcc
     6181 gcctcaagct cttcagggaa ctcgacttca agatgccgtt cgaccaggtg gcgatgtcgg
     6241 atcttcttga ttcaggcaag accatgaagg agatcagcga ggaacttgac gtgagccctt
     6301 ccacactccg tgagtaccgc catgtgatcg aacagcagag ggacaccacc ctggacccct
     6361 acctggaacg gatccgggac gtgatggaag accgtgatct ctccgagctg atgacgcgcg
     6421 gtgtcgccaa cgatggcctc tccgaggcga tcgacatcac cgaagcgatc gatatggggg
     6481 agttctgagc ccccggtcca ccctctgtta ttctactcaa cattttttac cgttcaggcc
     6541 gatctcttct ccatgaggtt gacctggctt ggccacgcat gtttctcact cgcaggatcg
     6601 cggacggtcc tcatcgatcc cttcatcccg gagggctcgc tcaccatgga gcccgatatc
     6661 gttgccgtga cgcacgccca cgcggatcac ctgggcattg ctgcgcagct gtcgaagaag
     6721 acggtcgcca tcaacgaagt ggcgaagtac ctgcgctcga aaggtgttcc cgccgaaccg
     6781 atgaacctcg gcggcagcat caccgtcgac ggtgtcgggt ttacgatgac gcctgcgctt
     6841 cactcgtcct ggcttgagga cgaaggggcg ggcttttacg gcggcgtcgc cgccgggttc
     6901 gtcatcaaga tggacggtat cagtgtctac cacgccggcg atacggcgct cttctccgat
     6961 atgcagctta tccgggacct ctatcggccg gacgtggcgc tcctccccgt cagcgggcgc
     7021 tacacgatgg gacccgacgc ggcgatgatc gcggcccggt acatcggagc gcggctggta
     7081 atcccgatgc actacgaccc gtcccctgcc aaccgggagg atctcctgaa cttcaaacag
     7141 gccctcgagc ggacgacagc gatccgggtc aggatcctct cgccggggga gagcatcgag
     7201 attggtcccg agagttccgg ggagtgaggc ctccacaccc cgggcctctc cgggagcacc
     7261 agggctggtg cggtcacggt gagcgtatgc ggcggggcgc ttttcaggca gcgtgcgtgg
     7321 tagagcaagc attttcgacg tagaaattgt cgggacggcc agaaattgcc acaagtcctg
     7381 gtacggtgga ccgtgggaat atcgccattg gggaggggct gacggggagg ggggcgtgcc
     7441 ccctcccctg tctccaattc gtacctttga tgctcaggct tgaggtgctt gccttccacg
     7501 tagcccccac cccccccggc cttcggcctc ctcccccgcc ccaagggacg ggggcagtgc
     7561 gtggcgatat gcgacgttct ggtaggaacg gagctcaagc accataggtg cgcagtcccc
     7621 accaaagccg ttcatgagat aggaaggtta tgggacacac aaagcctcct ccccacaagc
     7681 agaccacgaa cacccttcgc acgccccgaa caccccgccc ccggcaactc caacgtcagc
     7741 cccgccaacc tccaggcttt tccgccctcc ggaaagcacc ccaaaagaca cagcaccctg
     7801 tgcccccagc cccgccctcc accggatcct gcacaaggta tatgtg
//
LOCUS       DAJB01000108           12544 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_10035, whole genome shotgun sequence.
ACCESSION   DAJB01000108 DAJB01000000
VERSION     DAJB01000108.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12544)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12544)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12544
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_10035"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    6286..6337
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2086 a         4116 c         4080 g         2208 t
ORIGIN      
        1 ctccggagca ggagcggtgc ggcaagcagc gtcccggcaa ggaggtagcg ggaggcgatc
       61 cagagctgcg tcgggaggtt tgcgccgtag ccggcgaaga tccccatgcc gtcgtacgca
      121 agggtgtgga ccagttcgat aacggccacg aagaggagcc cggtgccggc gaccagcagg
      181 tagccgttct cccgcatcgt ccgggcgttc cacgccacga tgaagaccgc tatcgctatc
      241 gcgacggcaa cgagttcggc gatcgtgtgg aagagcaggt agttgtagag gctggtcgcc
      301 gcgagggcgg caagaagcac cccaaagacg gcgagcgagc tcccgatgcc gggttccgcc
      361 ggggcccgcc ggtcctctgc gccgttccgg gctgcacggg gtatcggttc tgtctgcgtc
      421 gagtcgtttc gcatggtcgt tcggatacgg ggcgggatcg tccgggtctt accgctgccg
      481 cacggcagcg gtgagcgacc ggcccacttt tattcaccat atagtatgtc tccaatatca
      541 ttaatttacc ggacagaaaa aacccgccag ggtgcgagat agaaagaatt agggcataat
      601 aaggggatat tttgggggag ggtccggtcc cgatcgggag cggctcccga tcgttacacc
      661 agttcgttcc gccgcaggaa ctcccgtatg tcgcgggatt cgacccagcc ctcgtcgagc
      721 tgtttgacga tcccgttgat ccgctcgacc tgctcccgga tcttttcaga catcctcgca
      781 tcgtcggcaa gatcggccat gccgaggatc acctgcaggg gcaggcggat gtggtctgca
      841 aggacggcaa actgctcgat gttctgctcg atctggtaga acgcctgcag ccgcaggtcc
      901 tcgtagcgct tccggtccga gacgtcccgg ccgatggtct gcacgccgac caccctgccg
      961 ccctccgtga tcgccgactc gttcatctcg acgacggcgg ccgtcccgtc cttccggcgg
     1021 aactccatca ccagcccctc gacgggctct ccccgaccaa tccgggccct ggcctcctgc
     1081 cagtcgggcc gggtccgatc gaggacgtag tcgctcgctt gctccccgat cagctcttcc
     1141 gggctatagc cgaggatcct cgttacgacc ggggagatgt aggtgatccc gcgatcggtg
     1201 tagcaggtgt agatcatatc gaagctccgc tgggcgatct cccggaaccg ctcctcgctc
     1261 tccgcgagag cgttctgcat ctcccgccag tcggtgatgt cctccaccat cgcgatgacg
     1321 agcccccgct ccccggagtc gtggagcagg gacgtggtca gccgccccca gaggatcctg
     1381 ccgtcctttg ttacgtagcg cttctcccgg cgggtgctct cgtccgaggc cgcctcccgg
     1441 tcgggcgggt agatcaggtc ccgggactgc atgccataca gttcgtcctc gtcgtagccg
     1501 aacatacgca cgaacgccgg gttcgccttc ataatcctcc cctccggttc gatgagcgcg
     1561 atcccggtcc ccgcctgctc gaagatcccc cggaaccgct cctcgctctg ccggagggcg
     1621 agttccgctc tcttctgctc ggtgatatcc tgccccgtaa cggccagcct cccggcctgc
     1681 gggcggtaga tctggagatc gtaccaccgg cggctcccgg gctcctgcag ccgccgctgc
     1741 accggacgac cggtctttgc gacctccccg tagagggcgc gggctccctt cgcgatcgcc
     1801 gggaagatat cgaagagccg tcgcccgacc agttcctccc ggctatggcc gaagacatcg
     1861 gccgccttct cgttcaactc gaggatacgg tagtcggcgg ggttcccggc gccgtccagg
     1921 acgacctcgt agagggtgta actctcgagc atctgctcga agagaaggcg atacttctcc
     1981 tcgctcctcc cgagcgcctc gtccgcccgc agccgctcga cgatccggcc gagcctcccg
     2041 gcgacggcgt cgagcaggcg gcgtccgtcc tccccgggca cctcgtggcg gtaacagatc
     2101 tccaccttcc cggtgatgcg gccatggacg acgaccgggc tctcctgctt ccagggggtc
     2161 tcgacaaacc cggccgaccg gtattcccgg ccgtcgacca cgatccgggc ggcagcatcc
     2221 tccgggtgga gccagccggc cgggaggatg gcggcaacct ccccgaggac ctcctcgacc
     2281 gatatcccgg gccgctcgac gatgccgctt acggcgtaga gcgtcgcaag ttccctcacc
     2341 cggttatcca gagcaatctg ctgcttctgg agcagctcat ccgctctccg ccggtccgtg
     2401 acatcgtctg caatgacgac gagccggtca tactgcggcc ggtatgcctt cagtctgtag
     2461 caggcgccgg tagcgggact ggagacctcg cggtgcaccg gtctcccggt tcttgcgacc
     2521 cgggagagga ggtcgagctc ggcagggccg atcgatggaa cgaccgtgac gaggtcttct
     2581 ccggcgacct cctggtggcg gcgcccgatc ctctcctcgg ccttgcggtt gatccggacg
     2641 acccggaagg aggcagggtc cccggagttg tcgcggagta tctccaggag gagcccggcg
     2701 tccggcatct ggtcgaagag gaggaggtac tgctgcccgg cggctgccgg cgcgacggcg
     2761 gcttcgacca tcgcggccac agcgcggacg aggtcgcgct cctgcgtagt gaaggggtct
     2821 tctgcctcct cccggcactc taccacgagg tgcccggcag ggatcccggc gacgatcctt
     2881 cccggccccg gagcggcgga gccggtcaca aactgcgccg acacctgctc cgggtgccgg
     2941 aacccacggg gcaggaggct gaccgtcttc tggatggcct ccaggggatc ggcgtccccg
     3001 atcgcccggg cgattgcgga gagcgtcgcc agctctctcg ccggtgcgca gggttcgtcg
     3061 agatcgaccc ccacccagag gacgacctgc ccggcggcgt ccctgaccgg gacgcccctg
     3121 ctcgcgacgg tgcggtactc cccgtcggcg ccccggatac ggtaactgca gctccacggc
     3181 gtgccggcgg cagcagagcg ctcccatccg gcgagcaccg catcagcgtc ctccgggtgg
     3241 aggctgccca cccatccgga gttccggatg ggtgccacgc cgtcgggccg gtagatccag
     3301 atcctgcagg ggcccgtccc gtcggcgagt cggtctggag gtcccggtct gtcccggggg
     3361 gtcgcggggt aatctggtgc aggcatcgtc tgggtccccg gtaaaggtgg ccagggtgac
     3421 ctggtatgtc gggctggccg gggagggagt tgttgccgat cacttggggc gatgtcgtca
     3481 taatgtttct gcctcccgcg ggggtgcgcc cggaccaccc tttatgtggg acggagccac
     3541 aactacacac catgatggag atctgcgccc ggagggggcc ggccggaggc agggcttgac
     3601 gggggatgag gagtgtgcgt cctgcccccg ctgccgggcc ggagtctgca ggatccacgg
     3661 gccggtcgtc ggccggcggg aggtcgcggt cttcgcggtc tcgggtctcc tccttctttc
     3721 ggggctgctc gccggatacc tcaccccgta ccccctcgcc ggcacggccc tgttcctcgc
     3781 cgccgcggtc atagccggtt acggtgtcct gaaggccgcc ttcctcgccc tcatccggtt
     3841 ccggttcagc atcgccgtgc tcatatccat cgcggcggtg ggcgcgtttc tgaccgggaa
     3901 cgccgcagag ggcgcgaccg tcctctacct ctacgccatc gccgagttcc tggaggagta
     3961 cgccgccggg agggcggagc gttcgatagc gtcgctcctc gatatcgcgc cgcagacggc
     4021 ccgggtccgg cggggcggtg aggagacgac cgtcccggtg agcgaggtcg gtgtcgggga
     4081 gaccgcgatc gcgagaccgg gcgacaccgt cgcgctcgac ggcatcgtca ccgccggtgc
     4141 gtcctcggtc gaccaggcgc cgatcaccgg ggagagcgtc ccggtggcaa agggcgtcgg
     4201 ggagaccgtc tatgccggga cccggaacat cgagggctac ctggagatcc gggtgacgaa
     4261 gcccgaagcg gagagcacca tagcccgcgt cgtggcgctg gtcgcggagg cccaggccca
     4321 cacctccccc accgaggcgt tcatcgagcg gttctcgcgc tactacacgc cggcggtcat
     4381 cctcgtcgcc gccctcctca tcgtggtccc gccgctcgcc ttcggcgtcc cgttcgtcga
     4441 agcgttctac cgggcgctga tcctgctcgt catcgcctgc ccctgtgcgc tcgccatatc
     4501 caccccggtc tcgatggtat cggggatcac gaccgcggcg cataacggcg tgctcatcaa
     4561 gggccgggac tccctcgagg ccgtggggct cgcccgggcg atagtcttcg acaagaccgg
     4621 gacgctcacg accggaaggc tcgaggtgga cgagatcatc gccttcgggg tgccggaggc
     4681 ggaggtgctc gccgtcgccg cgtcgctcga gtcccgttcc ggccacccga tcgcggaggc
     4741 ggtccggcgg cgggcggagg aggagggcac tcctctccgg gacgtggaag agttcgcctc
     4801 gctcaccggc agaggcgttc aggggcggat cggcaatgcg acctacctcc tcgggaacgc
     4861 cgcgctcctg gagcccgccg gcggggcatg gcgggaggag tacgaccgcc tggaggggga
     4921 gggaaagacc gtcgtcctcg ccggcaccgc atcagaggtc atcgggctga ttgccctcgc
     4981 cgacgtcgtc agggaggatg cccaacagac ggtggccgat ctccggaagc gggggatccg
     5041 gacggtgatg ctcaccgggg acaacgagcg cgtcggccag gcggtcgccc gccgcatcgg
     5101 gatcgacgag tgccatgccg ggctcctccc ggaggataag gtggcgatgg tcgaggaact
     5161 gatggaccgc tacgggctcg tggtgatggt cggcgacggc gtgaacgacg ccccggcgct
     5221 cgcgcgggcg aacgtcgggg tcgcgatggg ggccatcggg tcggacgtcg cgatcgaggc
     5281 ggccgatatc gtcgtgatgg aggacgacct gtcccgggtc gcctacctcg tggccctctc
     5341 ggagaagacg gtctcggtcg tacggcagaa cgtcacgacg gcgctcgtgg tgaaactcgg
     5401 catcgccggg cttgcggtcc tgggcctcgt ctccctctgg atggcggtgg cgtttgggga
     5461 tatggggctc tcgcttgcgg tcatagcaaa cgccctgcgg ataggccggg caggcagttg
     5521 aggaaaaaag gggaggtcgc tcacttccag agggcggtct tctcctgtgt ccatgccggg
     5581 tcgacaaacg agaggagttt cgtcaccgag tccgcggtgt tccgggactc cttcatcagg
     5641 cccggcggga cgtacgcggc actcccggca gggaccaata acgttcttcc atcgagcgtg
     5701 gtgacgagaa gctccccctc gatgacgtag atcaggtcgg acgagccaac gatcccgtca
     5761 taatcgaggt acccgcccgg ctggatctca ccgtatgcga cactgtaatt gatcggaagt
     5821 tccatctcgc tcatcagcac cgggtttgcg agggtgtaga cgaccgtatc ggtggcgctc
     5881 cactcgatcc ctttccgggg atcggcgacg acaaacggcg tctcattcgt cgtcatgctg
     5941 agcagggtaa gtgtctctcc cgagagctcg atctcggctg tatagggcgg ctggatcacg
     6001 gtgaggtaac ggagatcggt atccccggcc gaggctatcg actgcagcac cctctcgggg
     6061 aggagaacgg tctctccttc gcgggcggtc accacctggt tgtcgcagcg gatctccgcg
     6121 acgccgnngg atctgcgggg tctctgcgcc gatgagcccg acgtatgtcg cctggccgtc
     6181 gaatagggga accgggtctg ccggggtgat caggctgaca cccgcctcca ccccggattc
     6241 actcgcggtg aggcatccgg cggagaggag catgcaggcg aagagnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg agggtgaacc ggaacgccgt
     6361 cccctcctcc gggcagcccg ctacgcagtc ctcggcccag atcctgccgc cgtagcgcgc
     6421 agtaagcatc cggcagatcg agaggccgag gccctggctc ccccgcctgc ccccctcccg
     6481 ctcgaaccgg aagaagatcg tctccttcgc gtcgtccggg acaccggggc cggtgtcggc
     6541 aaccgtgacg atgacagtct tttcatccag gtcctcgacc gccaccgtga cctcgacgcc
     6601 cggacccccg tgctttgcgg cgttgccgat caggttcgtg aggacctccg gcaggaggtc
     6661 gtcggcgaag accgccacag ggagcccgtc gtagtggatg cggaggtcgg ggaagtgggc
     6721 gatctcctcc cggatcaggt cgtcgaggtc aacgggtgca agtccggccc ggcactcgcg
     6781 gatcttgcgg atagtggcga cgtttgcggt gatctcgatg ctctttctga tcccgtcttt
     6841 cagtttccgg gcgtggcggg ccggttcccc ctcgagttcg tcgatgagaa gatcagcgta
     6901 gatgtttgtg acgttgtcgg cgttccggat atcgtgggtg aggatatcga ggtagaggtt
     6961 cgcctcctgg tttgccgctt caagcctccg gtagagcatc ccccgcatga tgccggcccc
     7021 gatctcccgg ccgaccgcct cgagaagcgc acactcgtcg tgggagaaaa atccccggtc
     7081 cctgctcccg atgaggagca ccccgacgac gtcggactcg acgacaagcg ggatgcaggc
     7141 gagtgcggca agcccgagtt cccggaggag acgggaggcg gaggagtccg cctcccggtc
     7201 ctgttcgagg tagcagggaa ggccggcggc cagggtgccg gcgaatgacg acccgagcat
     7261 gctccccgcc cgctccaggc agagatccgg catgttccgc cggcactgga gcgtggtctc
     7321 tctctgcacg ggatcgatac ggtagatagc gccgccatcg tagccgagga ggtcgagcgt
     7381 ctggttgagt gccgtctcca gcagttctcc cggcagatgg gtcgatgcgg atgtgccgat
     7441 gatccggtta agcagcagga gatgccggtt ccttgcctgc aactgctctt ccgcgtgctt
     7501 ccggtgggtg acatcccgca taacgacctg gaccgccgcc cggccctcga taaacgtccg
     7561 cgtgcccctc ccctcgatgg gaaccgtcgt cccgtcgagc ctgacgaccc gcagttcagc
     7621 aagcggcgtc tcctcgccct gcagatcacg cacgccgagg gactggacag catcgcggac
     7681 ttccgggtgg acgatatcaa ggatagccgt cccgaggacc tcctcggggc ttgaagcgcc
     7741 gagcagcctc atgccggcgg ggttgatgta tacgatattc ccgtcgcggt ggatcaggat
     7801 agcctcggcg gagagttcga cgagcgagcg gtacttctcc tcgctctccc ggagctgtgc
     7861 ctccattctt tttaaggggg tgatctcctg gaccccggca aggaaccccc ggcaggtgcc
     7921 gtcggcgtcg cagatcgggg tggttgccat cagcgtatgg acccgggcac cgtttttatg
     7981 gacgagctcg aactcaaaga cctcgcgcac gttctggccc aggctctgca ggtaccctct
     8041 catgcacccg gcaccctcct cgtcggcgaa gagacagacc ggcgttccga cgatctcggc
     8101 gaccgggtag ccgatgatat cggccatcct ctggttggca aagaccacga caccctctct
     8161 gtcgatcagt ccgatgccct cgttgaggtt ctcgacgagg aaccggtacc gatcctcgct
     8221 ctcctgcagg atctcctcta tctgcctctg tcttccgtga ccgttgcccc ggaggatcgg
     8281 tgtgtcggcg atggaatcgg cctggagatt tgaagagagg cggatgatcc ggttgccgcg
     8341 aaacggacct tcacgaacgg tccggcacga gcagcggacc cgctcttccc cgccaccggg
     8401 cgaccgcagt gtgcagagga tatcggcagc cccggagcag tccgagaggg acgccaggac
     8461 ctccgccccg cccgattcct cgcagatccg ggggatcagg gtcccgcgga cgaacgtatc
     8521 cgcatcggtc ccaaagagtg cattcccgtc gatcgcaagg aggtgctgga gatgcctgtt
     8581 gatgcagacc acccggttgt cgggaccgac gatgagcagc ccctcgtcga gcgcatccat
     8641 gatgccggag agcgcgagga tatcgccatg tgtgggtgtg cattcatgca tcggccggga
     8701 ccaccttcct tttctccagg aggagtatca tcaccatgta cagataaaat gttcccgcac
     8761 acttcagaca gatcaggacc gtatccggag gaatttaggg gtcctagcgc atctgtgggc
     8821 aggttcatcc gagcacgggc agtatactct ttttctgaag gtataaatct gttcatttat
     8881 tcctgcaggt atccattgtc cttgagacaa tggctacgcc cccactacgt caccgcaact
     8941 gcgcgcggcc ggccgtgggc agccggtata tttacatccc gcgagagcgg catcaccccc
     9001 atggacgggt acgtgtacca ggacggccgt caggtcctac acggcgagac cgacgaagag
     9061 gagccggtgg agaggggctg agatggacca cgatcacggg gcgaccggag atcgccacga
     9121 aacgccgccg gagacccacc ggcccggggg cggccggggc ggtgctgatg agcgcaagca
     9181 ccgtgatcgt cgccatcaac gcccggctgc ttcgggtata ggaacggtct ctgtaccacc
     9241 gcccgggtga ttgattgaac cgggaagttc catgcggtca aataggagag gatcgtcagt
     9301 atctatcgga gacgagttaa cctatgaaac gcgacactac aatccgcatc ttctccctgc
     9361 tgatcgtcct tgccgtggtg gtccctgccg tgcaggccgc ccttgtcatc gacgcaggga
     9421 acgaccgcac cgcactcgcc ggcgagccga tcaccatcct cgctgcatac aacggcaccg
     9481 acgtcttcga cgcgctgaat atgagcgcct ccgtcaactg gagcacggca acgacggcac
     9541 cggagatcgt gccggatgac gactacaacg gcaccgtaca ggccacctac acctacctcg
     9601 ctgccggcac ttacaccgtc acggtggagg tcgcgaacaa cgccacgggt gccgtcgcct
     9661 gcgacaccct gaacgtgacc gtgagccgcg agcccgcaga ggtgaaggtt gtcccgaaga
     9721 cgctgaacca gaagagcaac ggtgtcatga ccatcttcat caacgttgca gagtggctcg
     9781 gctttgagga tgccaacagg acgaacgcga cgttaccgga cccctcggag ttcaggatcg
     9841 ggaacgcaac gccggagaag gtcaagttca acatgaagga cggcggcacg ctcatgctga
     9901 agtaccggcg agcggacctc gacctcaacg ccggtgacgg caacctgacc gtgaccggca
     9961 cgacagagga tgggaacatc ccggtggtgg gcagcggcgc cgtatccgtg atcaacccgg
    10021 gcaaaaacgg tgagaacagt cacgggtatg ccgggaccgc gaaggagaag gtgaagaacc
    10081 agaaagaatc cggtgaagcg caacagcaat ccaacggaaa cgcaaacgga cgggcgccgt
    10141 aacggtgcac cgctcttgct gcccggcaca gctccgggat caatccgctt atcatatttg
    10201 gggtcgacca ctaggtacat gggacgatac gtcgctggag acgttcttct ggcctccgtg
    10261 cgtatcgggg gcgtcggtga acggaaggtg cggccggtgg tcgtcgttat ggcaggagag
    10321 agcggagacc tgctcgtctg cccggtctcg agcagaccgt ccatcgacgc gccctcggtc
    10381 ccgctctcgc tcgacgactt tgcccggggc gggctcgacc tcttcatcga gagctacgcc
    10441 ctgaccgcat acgccgccac catccggacg gcggacgtcg tcgggaagaa aggcactctt
    10501 ctgccggagc ccctcgccgc catccgggag gccgtgccgg cgatgcacga aaagaggggg
    10561 cgccggcggt agtgatcttc agagttcgta ccgcttcagg aactgccgga tcgcccggga
    10621 ctccacccat tcgcggtcga gctcgctgat ctgcacgttg atcctccgga cctgctcccg
    10681 gagtttctcc gcagctttct cgtcgtcgag caggtccgca acccccatga ccacctggag
    10741 cgggtgacgg acgtggtcgg caaggacggc gaactgcgcg atgttccgct cggtcatgtc
    10801 gaacgcctgc tcccggagcc gctcgtacag gatccgctcg gagatgtccc tgccgacggc
    10861 ctgggacccc acgaccgtcc cgttctcgac gatcggcgag acgttcagtt ccagtatggc
    10921 ggtctctccg tccttgcgcc tgacctcgac ctgaagccct tcgacctgct cgccccggag
    10981 caccttccgg cgcccctcct cccataccgg aaggcttgat gggaggacgt aatcctccca
    11041 gacggtccct ttcatctcct cgggggagta cccgagtatc cgctccatcg ccggcgagac
    11101 gtagttcagc ccgtcggcaa ggtatgctgt aacgatgagg tcgaaactcc gctgggcgat
    11161 cccccggaac ttctcctcac tctcctcgag cgccgcttcc acctgcagcc gctcgatgat
    11221 ccggccgagc tgcttcgtga cggcatcaag cagcatccgc tcttccggca ggaatgggcc
    11281 tccggctacc ttcggctttt cgcagcggta ccggacctcg acctcaccaa cccttgcgcc
    11341 acggacgacg atcgggcatt cctgaatcca ggcggcttct cccgggtcac cccggcggta
    11401 ctcccggccg tcgacgctga tccgtaccgc ggtctcgtcc gggtgctgcc agcccgaggg
    11461 gagggtatcg gcaaccgcct gcaggagcct atcaagcgtc atccccggca cctccacgag
    11521 gtgcgcgacg gtgtacaggc accggagttc gtccatccgc ttcttcagtt cgtgcgtctt
    11581 ctcgcggagc gcctcctccg cccggtccct ctcggtcaca tcgaaggctg tcccggtgac
    11641 gccgacaacc ttcccctccg cgtccaggag cggctttaac gtcaggtccc ggatgcgcgg
    11701 accgtccggg acgatgaacg tcaccgccgc ccgcacggtc tctcccgtct ccagggcccg
    11761 gcgcttgagc gcggtaagaa gcgcggcatt ttcaggaaag gcctcctcgt cccgcatgcc
    11821 gatgatggag gtatccgtgg accccagctg ctggttgtac gaccagatgt agcggaggtc
    11881 ctcatcctgc gcaaagacga ccaccggcga ctggtcgagc gcggctttga agtggtgcgc
    11941 atgcagatct gcggtggcat cccggatcct gatgagccgc tgcccgtcgg ggaccggtac
    12001 gcccgagatc gagagccagc gccgggcgcc gtcgggtctc cgggcacggc agaggagggc
    12061 aggaaagtct gcaccgttct ccagagcctc cagaagcagc cttgcggggg cttcatcatc
    12121 gagggtcggc agaaggtgct catcgaagag agagcgtacg tccgtcccga cggcgtcttc
    12181 ctctccgatg gcaagagtcc gtgcaagggc cgggttgtac cagctgaccc tccggtcatc
    12241 gcttaccacg agaaggccgt cctcgagagc gtcaagcacc gcgcgatcgc agagtgcccg
    12301 gtcctgatct gagtctgtca tttcccaccg gcgctggaat gcagttgctg tagtggttgt
    12361 gctccagggg gataaaaaag attactaccc gggttgtggg cagaaatcag gtttttctca
    12421 gtcatttaag tgttttttcg gcatccctgt ctcgtaaacg ccgggcccgg gtgcatcgtt
    12481 ccgggtaaga gacccggcca acaggtccac tcagatgtca actaccccgc cctgaaggac
    12541 gggg
//
LOCUS       DAJB01000109            7707 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_78726, whole genome shotgun sequence.
ACCESSION   DAJB01000109 DAJB01000000
VERSION     DAJB01000109.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_78726"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1465 a         2328 c         2406 g         1506 t
ORIGIN      
        1 atcatgggat aaatatataa caagagagca ctaccttaag gttagccccg gcaacctgtt
       61 cgcccggggc catcccagtc gataggagtg attacaatgg caagaatcga acgaggacca
      121 taccggacaa tatggcagga tttcgacgac cttatggccg agatggagag caggttccag
      181 tccctgctcg ggggaatcgg cacacgggga gaagagatca ggggccgggt cgtcccggca
      241 gtccgtgatt tccgcgtcga tgtccgggac cacgaagacg aggttatcgt cgttgccgac
      301 ctccccggcg tcgagaagga gaacgttgcc gtcaggctca ccgatccccg gcacctggag
      361 atcctgagca ggcagacggg cgagagccag gaggaggcga aagactactt catgcgggag
      421 cgcatctacg gccagatgag ccgcacggtg ctgctccccg ccgaggtgac cgaggaaaat
      481 tccgccgctt cgtttaagaa cggagttctc gaggtccggc tgaagaaagc gccgaccgag
      541 accgggactg caatccccat tgagtaacct ttttttccgg tcttccccta atttcaaccg
      601 aataattcat gtcgagccag cgtgacacta tcatgatgga taaaaagaag gtcaaggatt
      661 acatgaccta cgacgtcgtc accgtcaacg cccacggaac ggtccgcgac gtcatcgaga
      721 ctataaaaaa gacgcatcac gacggcttcc cggtcgtgga gaactcaaaa gaggtggtgg
      781 gctacatctc ggcgcgggac ctcctctttg cccatccggc aacaccgata gagcaggtga
      841 tgtcccgcca cctcatcgtc gccgaccccg acatgagtgt gaacgatgca gcacgcgtca
      901 tcttccgttc cggcatccag aaactcccgg tcgtcaacga gaagaacgaa ctcatcggga
      961 tcatgtcgaa ctccgacgtc atcaggtccc agatcgaaca cgtctcgccc gagaaggtct
     1021 tcaagttcat cgagacctta aaaaaactct acggagtgga accgatgctg aggcgggggt
     1081 cggtcccgat caacgacctc ctgcccaccc agtccaagat ctacgaggac gaactggaag
     1141 ggcggatgta cgagatccag aaagggcttg ccgaacccct gatcgtggtc cgccggccgg
     1201 gccgctggat cctggtggac gggcaccacc gggcgatcgc ggcaaaacgg ctcgggatca
     1261 cgaacctcga cgcctacatc atcgaggtcc gggagaatat agagttaggg atggagcgga
     1321 cggcccggac gctgaacctc agcacgctcg acgacatcaa ggtgctcgat tacgcccgcc
     1381 atcccctcgt cgcgctgacg caccggctcg tgcggcacgg ttaagagcct tcggtccggg
     1441 tgtcgtagtc ctcgttcaag acgtgctctt tcacgattgt cccctccggg atgacgacct
     1501 ccggccagag ccgggtcccg gaatggacga ccacgccgcc tttcagcacc gcatgcggcc
     1561 cgatgaccgt atcgtgctcg atgttgcacc cgctgccgat gaacgtatcg ttgtcgatga
     1621 tgctcccgct gatggtggtg tccctgccga tcaccacccg gttgtagatg gacgacgaga
     1681 agatcttggc gctgttcttg atgatgcacc cctccccgat gctcgtatac ggcccgatga
     1741 cgacgttctc ctcgatgatc gtcccggcgc cgatcgagac cggcccgatc acccgggagt
     1801 tcgccgcaac cgagacgcag ctccctatct gcgccgggcc gaggatccgg gcgcccttga
     1861 tgtagaggtc gccggagatg ttcgtgaacc cgatgtcctg cagtttccac cgctccgctt
     1921 cgcgaagcga cctcgggctc cccacgtcgg accagttgcc gcgggcaagc caggctttta
     1981 aggcgtagcc ttcctccatc agttcgggga agaggttgcg ggcgaaatcg aacttctcgc
     2041 cggccgggat gtggtcgaat atgtcggggt cgcagacgta catcccggtg ctcgccaggt
     2101 tcgagaagat ctcccccggg cttggcttct ccttgaacct cttaatctgg tagttcgcat
     2161 cgatctcggc gatgccgtac tcggtggggt catcgatgct gatgaggccg atcgtggtga
     2221 tggagtcgtt cgtcagatgc tcgcggtaga actcgagcag gttcaagccc accacgtggt
     2281 cgccgccgac gacgaggaac ggctgctcat cgaggtattt ctgagcgttc ttgacactcc
     2341 ctgccgtccc gagtttcgtc ttttcgtgga cgtaggtgac gttgacgcca aagagcgacc
     2401 cgtccccaag cgcctcttcg atggcttcgc tcatgtagcc gagcgtgatc accacgtcgt
     2461 tgaacccgag gttagagagg tgtgagacca ggtgctggat cgagggtttg ttgacaatcg
     2521 gaatacacgg cttaggacgc ccgaatgtga gggggcggag ccgtgtgccc tcccctccgc
     2581 acattatgca caccttcatg acactccatt gtatgcgtat caatttaacc ctattgggcc
     2641 gatccgaccg ttcgttcccg aacctttatt ctctgttgtg caaagattct acagacgatg
     2701 acggcgtttg aatgtacgct ctgtgggaaa tgctgcatgc acgcgggcgg cgaactcatc
     2761 gagatcgaaa aaaggctcac cagccgggat tacctctgtc ggcagaagat cgtcggcggc
     2821 acgttccgcg cccgggtcga ggagcggttc ctcgatgcct tcagggatac ctcaaaaaac
     2881 gataagcacc cgtcctggtg cccattcctg cgcggcctcc cgggagagga gggaaagtat
     2941 gtctgcaccg tccacggcag ccgcccgctg atctgccggt cctacttctg ttgtaccatg
     3001 cggatctttg ccggcgacgg ccgggaggtc gggagggtga aggggaggag gagtctcgat
     3061 accgaagacg acgccctgcg ccggtgctgg gaggagtcgg ttgtaccgct tgggactgac
     3121 gacgatattg cctggcaaag cgaggtcgcc atgatactcg ggcgtgccgg ctacaggatc
     3181 gatgcctatg agtgaggaaa ggtacctccc ggtcgtccgg ggccgggtgc agtgcaccca
     3241 aaaagaggcc acctccatcg acctgtgcag gctctgtgtc cattctgccc gtgtcgtggt
     3301 caaaggaaga gaactgccgt caccggcacg ggcctattgc agccggtgcc gggagatacc
     3361 tgacgtcgat atggcgaagg tcgaggcaat agtctgcgac gacctctcag gcgaggggtt
     3421 ccggagcatt acaaacatca tcagttagcc ggttacttcc gccagaactc ccaggggtgt
     3481 ttcgtctgga acggaacacc ttttgcctcg tgattccagt cggtctcgat cgtgtggtgg
     3541 accagcctga cctcgttccg gagctcggtg agaacctctt tgacgtcctc aagttcttgc
     3601 tggagccggt tcaactcttc aaacgagcgc tgctgccgta ccgtcaccgt cccgccatcc
     3661 ttccggcagg caagaccggc ctcgatcaac tcaagaatgg cctcgttccg gtcgagcgcg
     3721 tggtcccggg caaactgatc gatcaactcc atcaggtcgg gatcgagcgc aaacgaacat
     3781 ctcatcacca tagatcgtcc actcctgagg atattgtatc cctctggtga ttatagtgtc
     3841 tgccgttccg gtatggcgta tgtcatccgg gcgggcaagg gatggcatcc actccctgtg
     3901 caactctttt attctgcaaa aaaaagggtt cccgggtcct ctatcgagga cggcccggtc
     3961 agctgatatt ttccatgttg tatcctttct gagcgagcag gtcctttacc cgctcgcgat
     4021 ggttgccctg cagctcgatc gaagaatctt tgaccgtgcc gccacaggcc agtttcgctt
     4081 tcaggaactt cgagaggtct tcgagatcga tgtcgtaggg gtcgagaccc tcgatgaccg
     4141 taacttcttt tccataccgg cgtctgttta tctttacgct gattctctgt tgttctttgg
     4201 caacttcttc acagatacac agttcctttg gcagtccgca ggtcggacat atcccaccgt
     4261 tcattgcatg tacctttcag ctctcgttat atattatttg gcatgctcat ccgtcgatac
     4321 ggcagacgtc gcaccctagc gagagtatct ggtcgggccg gctccggtag cggtagacgg
     4381 tgatgccctt gcacccgagg tcgcgggcga gagagaagac gcgggcaatc tcatcgacgg
     4441 ttgcactctc cggaaggttc acggtctttg agacggcgtt gtccacgtgc ttctggaacg
     4501 cggcctgcat ccggacgtgg tggtccggtg cgatctcgat ggccgtctta aagaggtccc
     4561 gggtatgggc atccaggggg aggccaacga ctgtgccgta gcgccgcacg tgctccgcca
     4621 cgtctctccc gccggcggtc tccggcagga gttcccggac gaggtcgctc tcgatgctga
     4681 ccggctgccc gtcgatcgtc cggtcgtagg caaaggagaa gaccggctcg atgccgctcg
     4741 tggttccggc gatgaggtgg agcgagcccg tgggggcgat ggtggtgacg gtggcgttcc
     4801 gcatatcccc tgagaagacg gacccttcga tcgccggaaa cgaccccttc tgtgccccga
     4861 gttcctccga gcgctcccgg gccgccgcct ggacctgtcc catcaggctc ccggcaaacc
     4921 ggatcgcctc ctccgactcg tagggtatcc cgaggcgtat gagcgcctcg ctaaatccca
     4981 tcaccccgag gccgatcttg cgggtagcca gggttcgaac ccggatctcc ggcaggggga
     5041 agcggttgac gtcgatgacc gcgtcgagga agtcgacgcc gcaccggact accgtggcga
     5101 gcaggtcttc gtcgaggtcg tgctgccgta tgcaccgggc gaggttgacg ctcccgaggt
     5161 tgcagctctc gtaggggtag agcggctgct cgccgcaggg gttggtcgcc tcgatggccc
     5221 cgaggtgcgg ggtggtattt cgccggttga tctcgtcgag gaagagcacg cccggctcac
     5281 cggagatacg ggcggatgcg gcgatgagac gccagagcga tctggcatcg atgctccgcg
     5341 agacgctgcc gtcgcggggg ttgatgaggt cgtactccgc tcccttctcc aggcaccgga
     5401 ggaacctctc gtcgatgccg accgagatgt tgaagtttgg gaaacctcct gcctgcttgc
     5461 aggtgacgaa ctcctcgata tcggggtggg atgccgcgag cacccccata ttcgcgcccc
     5521 gccgccgtcc gccctgcctg accgcttctg tcgccgcatc gaagactcgc atgaacgaga
     5581 ccggcccact tgcagcgccc acggccccgt tgacgacgtc gccgcggggc cgcagacggg
     5641 aaaacgagaa cccggtccct cccccggacc tgtggatgag tgccatgtgg gcgaggcttg
     5701 caaagatttc ttcgagggta tcctccaccg ggaggacgaa gcaggcggag agctgccccg
     5761 aggcggttcc tgcgttcatc agggtcgggg agttcgggag gaagaggagg cttgagacga
     5821 gcgagaagaa ctcgcgggac cgtgccgggc cgcccacggc gtcggcgacc cgggagaaga
     5881 ggtcgtgcgg tctctcgccg gggaggaggt accggcgttc caggaggagg cggccttgcg
     5941 gggagaactc catacagcat catgaaaccc cgcccttaat aactcgtcgt gcccaactct
     6001 cctgccagca atgtctctta tcgtgctcgg gactgcatcc cacgtgggca agagcgtgac
     6061 cgttgcggcg ctctgccgtg ccctctaccg gcgcggaatc ccggttgcgc cgttcaaatc
     6121 ccagaacatg agcctcaact catacgttac ggccgacggg agcgagatcg ggatagccca
     6181 ggcagtccag gccttcgcgg ccggcgtcga gccggtagcc gacatgaacc cgattctcct
     6241 caaaccgaag ggagaccagg tatcacaggt ggttctgctg ggccagccat acaaggatgt
     6301 gcagatccgg gactactacg ccgagaccga tatgctcctt gaagaagcgg tctctgcgtt
     6361 tgagcgggtg cggagacgct ttgggcacgt ggtggtggaa ggggccggag gagcggcgga
     6421 ggtgaacctc tacgaccgcg acatcgcaaa catacggctc gcccggcgcc tcgccctccc
     6481 catcgtcctg gtcgccgata tcgaacgggg cggggtcttc gcccaggtct acgggacgct
     6541 cgccctcctc cctgaggata tccggcccct cgtcgtcggg gtcatcgtca acaagttccg
     6601 gggagacgcc gggctctttg catcgggcgt tgtaaaactc gaggaactca cgggcgtccc
     6661 ggtgctcggg gtggtgccct atacggatat ccccctcccg agcgaggact cgctctcgat
     6721 cgccgacaaa gggcaccggg cgaccgggcg gccggtcagg atcgccgtcg tccgcctccc
     6781 ccggatctcg aacttcaccg acttcgaact cctcgaacag cacgcctcgg tggactatgt
     6841 cctgccgggc gcatcgcttg caggctacga ctgcatcatc ctcccgggga cgaagaacac
     6901 cgtggaggac ttcgccgtgc tgcaacggca cgggacggga gaggaactcc ggctcgcccg
     6961 ggagcggggg gtcccggtca tcggcatctg cggcggctac cagatgctcg gccgccggat
     7021 cgtcgacgcc ggcatcgagt cggacacccc tgccgattat cgtgggttcg ggctcctcga
     7081 cgtcgtcacc gcctttaccg gctaccacaa gacgacggtg caggtacggc gccgggcatc
     7141 cgggcccggt cccatcctct cgaggatggg tgaggtggac ggctacgaga tccacatggg
     7201 cgagacggag cggggagatc tccccgaggc gttcgccggg gagggcgccg caacccccga
     7261 cggcctggtc ttcgggacct acatgcacgg cctcttccag aaccccggtg ctgtaaacgc
     7321 cctcctcgcc tatctatcga aacgccgggg gctgccgttt gagcctataa cggaggcgga
     7381 cggcggcgca ttgggcgcat ccgcagcata cgatgacctg gccagacatt ttgaggcgca
     7441 tgttgatatg gatacgatcc tgggcttttn ngcccggcac ttcgaggagc acgtggatat
     7501 ggacgcgatt atggagtatt ttgtggatgc gacggttcgt tagaaccgga gcatgagaaa
     7561 acgcttcgcg ttttcgaacg gcgattgggc gaggtgagag gcgcccgggg tgagcacaca
     7621 gatataccaa accctgtttt ttccgggcat gtcctggtac agtggaccgt gggggaatcg
     7681 ccatggggga ggggtttcag gggaggg
//
LOCUS       DAJB01000110            5297 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_24228, whole genome shotgun sequence.
ACCESSION   DAJB01000110 DAJB01000000
VERSION     DAJB01000110.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_24228"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1017 a         1611 c         1680 g          987 t
ORIGIN      
        1 atcagttgtt tcgtagggca ctaccagcgt ggcggtgggg tggagatcct gacgggtgga
       61 tcggaaaccc tgtccggccc ggccgaaccc ttccggtccc tcgcgatcac accccggacc
      121 gggccggcag atacgggagc ggtcaggtga gtggtggaac catgacggcc ccggggtgca
      181 gagcccctcc gtcgggtgcc cttcctgcct gccgcaccac cccggtcgcc ggcccttctc
      241 ccccggaaag caccctcttt gctgctggtg gaagtacagt ccggtgaaaa acagttcgat
      301 aatatccgtt cgatgattta tccatnnttg aaaggggttc gcaaagtgaa atccattctc
      361 ttaaaactat cataatcgca agatctccca ttgaaataat aatattttta tagtcagatt
      421 taaaacaata gatactatct gatggtggat ggatgaggtc agacaaggta cggtatttta
      481 cgccacgcga tgaagaactc gccgagctct taattggcat cggtatcaag agaaacgtct
      541 ccaaagtgct cgtatatctt gcgaacacgg aggaagcgac atcccgggac atcgaacggg
      601 gcaccgatct ccgccagccc gaagtcagca tcgccatgcg ctacctcaag gaatgcgact
      661 ggatcgatac tcgcgagagc aagtcggaga gcaaagggag gccggtgaag atctatacgc
      721 tctcccgttc gataaccgag atcatggata cgattgaaaa ggaaaagaag aaggaagccc
      781 ggcaccagtt agaccttatc gagaagatgc gggaatgcat atcaacgcag tgatacgacg
      841 cggcacccct tctcctcacc aacaataacc attatctttt ctccgtaggc ggtaaacagc
      901 ccgcactcca gcgcaccggg tatcgaggct atcgctgctt caaggccccg ggggtccgga
      961 accgccccaa agtcacagtc gaagatgaag ttgccgttgt cggtgacgac cgggccgtct
     1021 ttcttcaccg cttcccggat gaccgggctg ccaccgagga gagcgaggcg cctgaagacg
     1081 ggcgccgagg cgaacgggag aacctcgacc ggcacgagtg cgtccagggt ttctaccatc
     1141 tttgtggagt cgactacgat gagcactttt tgggccgcgg cgcagacgca cttctcccgg
     1201 aggagcgctg ctccgcgccc cttgatcagg accaggcccg ggtcgatctg gtcggccccg
     1261 tcgatggcga tatcgagttt gggatgctca tcgaggctcg tgagcgggat accatactgg
     1321 cgtgcccgga tggccgcctg gtaggatgtc ggaacgccgg cgatacaaag gccgtcctct
     1381 gctatccgct cgcccagccg ctccatcgca aagaagaccg tcgacccggt gccgaggccg
     1441 acgaccatcc cgtcctcgac catccccgcc gcccgatacc ctgcgttctg cttcgacgct
     1501 atctgctctg ctgcattcat gacgtactct tctccttccg ttcagatatt cgtgccgtca
     1561 aagatgcccc tgagttcatc cgtggcacga aacgccgtgc agtcgagcgt gatgggcgtg
     1621 acggagatgt tgcctttctg caccgcatgc acgtcggtcc cctcctccgc atcctcgtgc
     1681 agcgggccgt cgatccagta gtaggggcgc ccgcgggggt cgaggcgttc ttccacgccg
     1741 gtgtagaaga gtttctcggc aagacgggtg atctcgtagc cgccgcgcac cggtgccggg
     1801 atgttcacgt tgatgacatg agcacccttc gggaacccgt ttttgaggat cttctcgcag
     1861 acctcacgaa ccacccgctt tgcatcgaag aaccggtccg tgatcctcga ggggtcgtcg
     1921 aacttctcgc cctggtcctc cacctgcagc gagaaggcaa gggacggcac gccctggttg
     1981 gacgcttcga gtgcggcccc gacggtgccc gaggtcatga tggactcgta ggagaggttc
     2041 tcgccgatat tgacgccgct gaccacgagg tccgggttca ggtgcagcgc aaacagcccg
     2101 atgatcacgg agtcggtcgg tttcccgccg accgagtatg ccggcacccc gttcatcgtc
     2161 acccgggttg cgcggatagg ctcgaagatg gagatcgatc gtcctacggc gctctgctgg
     2221 gtggcaggcg cgaccacggt gacgtcggct atcggcgcga gcgcgtcgta tgccgcccag
     2281 agtcccgtgg acgtgatccc atcatcgttg gtgagcagta tcttcggttt catccttatc
     2341 tatagggaag gttcccgcgc atcaaatact tgcccgatcg tcagggtcaa atctattgta
     2401 gccaaccagt actgcgatga aggcaattct cgccgaatac accgtatgca acgaccccga
     2461 gcttgcgccg gaaggcgctg ccatgctcgc cgcgatacgc gagagttttg agaggtgcgg
     2521 ctacgaggtg gtgaccccgg agcggcccga ctttgagggc gagatccgga ggctcgcgcc
     2581 ggggtgcgat gtggggctgg tgattgcgcc ggaccgcctc ctcttccggt acacgcagat
     2641 cctcgaaggg ctcacccaca acgtcgggtg cgggagcatg aatgcggccg tctgcgccaa
     2701 caaacagcgg acggcagcga tcctcgcccg gaacggggtg gcggtccccc ccgatgccgg
     2761 cgaagggagg aggatcgtca agcccgttac gggctgcggc gcccagggag tcaggctgac
     2821 ggacgaggag ccgggcgccg gcgagttcgc ccaggcctac gtcgaggggg agacgctgag
     2881 cgtgagcctc gtggggagcc gggtgaccgg caacgtctgc gagtactact cgggcaaccc
     2941 cccgctcctg cttgccgtca accggcagga gatcgccgtc ggagacgacg ggagggtccg
     3001 gtacctcggg ggcgagaccc ctgtccaccc cgaccgcgag gaagagatcg tcgaggtcgc
     3061 cgtccgggcg atgaacatcc tcgggtgcca ggggtatgcc gggatcgaca tcatcgccgg
     3121 agaccagatc tacgtcgtcg acgtcaaccc gagacccacg accagcctgg tcgggatcgc
     3181 gagcgtaatg gaggaggaga tcgccgatat cctcgtcaaa gcatcgcacg gcgaagcgcc
     3241 ggcagaggtg cacctctccg ggagcgtgcg gttcgataag gacgggagga tcagccggct
     3301 atgatcggca tcgatatcgg cggtgcgaac ctcaaggtcg tcgacgacgc cggcatgcat
     3361 atccactact gtccgctctg gcagggcgcg ccgctcgccg agcttctcga accgtacgcc
     3421 gaacctgcgg ccgtggtgat gagcggggaa ctggcggact gctttgcaag caagatcgaa
     3481 ggcatccggt ggattgtcgg gaccgtccag gcggccatcc ccgacgccgc cttctacggc
     3541 accgacgcgg cgttccatac ccggccggtc ccggcccttg ccgccgcgaa ctggctggca
     3601 tcggccgact acctgcgaga ggagtatgcc gatgcggtgc tcctcgatgt ggggagcacc
     3661 accgccgaca tcatcccgtt gaactgtttc gagagtctca aagggctgac cgacacccgg
     3721 cggctgcaga agcagtacct cgtctacacc ggcatgctca ggacgaacgt cgccacgctt
     3781 ctctcggcgg tgacgctgga tgggacggta accccggtga gcaccgagta cttcgctgca
     3841 agcgccgacg cgcacctcgt gctcggccag atcgctccgg aagactacat ctgccccgca
     3901 ccggacaacg gcgagaagac ccgggatgcg gcgctccgga ggctggcccg ggttgtctgc
     3961 gccgatctcg acgagatcgg gagagacgga gccacccaga tcgcccggca gttctgggag
     4021 acccagagga cgctgatcgc ccgcgcagtg gggcgcgcac tcttccggag cggtgccggg
     4081 cgggtgatca cagcggggat aggtgcggac ctctttgccc gtgaactcgg cggcgtcacg
     4141 cttgaagggg agatcgggga ggtctcggat gcactgccgg cctacgccgt cagggaggtg
     4201 gcgctacggc aacgcgcctg cgactgaccc tcctcctgct ggcggtgtcg gtcgtcgtca
     4261 cggcactctc agcgatcctc ggttttccgt tcttctttct ctttctcttc ataccgctga
     4321 tcccgctctt tgggcgatcc cccgtcgtga ggcgctgccc ggcctgcgga tgggaaaccg
     4381 caggcgacga gcgcttctgc ccccgtgatg ggatggcgct cacgcccgag ggtgaaggtg
     4441 gcgaggttca gcagagagag cggtagaagg aggccgaaga gttcggttgc cgggatcgtg
     4501 ccgaagtcgc gggaggcgta gttccggatc gtcccccgat caacgacgat ccccatcgcc
     4561 cggaggcgtt gtgccacccg gttgaagggc atctgctgcg acagaaggag acagaggtcg
     4621 acgacgggcg atgcgaggcg ggtgtcgggg tagaacggtg cgtctgcgta gcagagtgtg
     4681 ccgcactgct ggcagtagaa ccgtttcacg gagacccgga tggtgcgttc ctttccgttc
     4741 tcgagcagga cggcaaaccg ttttgcccgc aggtcatgcc cggtcaccgg accgccgcac
     4801 gacgggcagg cccggaggta ggtgaacttc acgccgtcca ttgacgccag ggcgtttccc
     4861 accaggtcgg cgagcatggg cggaaccggg agtgtctttt ttacaatggt aactctctcc
     4921 ctgtgcaact gtgcgagagg tgtaggcgtc caccaataat aacctcttcc ctcgaacggg
     4981 agtgggcgac acagaggtcg ttccagccca cagggtggtg tcccggagag cgcacgtttt
     5041 ttgcctgccc tgccgttccc gcttcggttc ggggcaacca tcctgacccc gcgaggggct
     5101 catcgagccg ccgcccggag attggtttag cacttctgcc tgatctcgat cgggatgcaa
     5161 acctgtattc tgatgggtat tactcacgta aaaactcaaa aaatgaatgc ggattattat
     5221 tctccagcca gtcctgcctg tgaagatgag ggcagatctg gttaactact ataatttaag
     5281 tagcttaaca tcagata
//
LOCUS       DAJB01000111           36411 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_35905, whole genome shotgun sequence.
ACCESSION   DAJB01000111 DAJB01000000
VERSION     DAJB01000111.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 36411)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36411)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36411
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_35905"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    5529..5562
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5752..5810
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6362 a        11382 c        11511 g         7061 t
ORIGIN      
        1 cgacgtcata accagctaga ccacgagccc catgcatcga ccgggaattg aacccgggct
       61 attggcttgg aaggccaaag tcataccact agaccatcga tgcgtactgg tacgtctata
      121 caattctggc gttatgcgat attaacatat ccccgggggc gtcggccccg tcgccaggtc
      181 cggtgcctcc ttatgcctgg attctggcac ggccggcggt ggtccggcaa tagaacctat
      241 ctgtcagccc gccgcggaac cggcggcggt caggttgtcc cctcccgccg ataccgtcag
      301 gacgaactcc ccccggtcgg ccaggggcga gacgacgatg accgcctccc cgtcctcgtt
      361 gacgaccttc tccatccaga agtcccggtc gtttgtgtac gagactgcgt cggggtccag
      421 tgtgcgtatc tcgtagtacg ccgcggacca gggagggagc tctgtctccg ggaacgagtg
      481 caccgatccg gtgaggtcgg cccggccggt cacctgtgga taccgggtga gattgatccc
      541 ggcatacccg agcggcggct ccctcccctc ggcatcgagg taattggcga ctatccagcg
      601 cttgaatacg tcctcgaacg actccgaaaa accgtgcagc gcgagcgttg cgtcgatccc
      661 ccgcaccccg gtaaggttct ccgccacgag gtccgcgagg aatgtcctcg tctccgggtc
      721 tcccaacggt ccccgaagtt ctccgcaagg tagagggtcc agaggaagct cgccccgtag
      781 tgggcctggt tggtctcgcc ctccgaacgg gtccactggc catcggtcac cgcgaggggg
      841 gtgtcggggc ggcggttgaa ggcccggacc ttcgcccggt tctcggtgtc gaagcagagg
      901 aactccgcat acccgctgca cccctcctcg acccaggtcc gctcgtaggg gtcgtgcgag
      961 tggtggacga ggtgctggaa ctcgtgagcg agtgtcgatc tcacttcgct ctcccgtgca
     1021 tccgcgtcga gaaagagcat atccaggttg tttgcggtgc tgccgtcaaa atatccggct
     1081 acaaagaggt ccgtagcatt atccccgggg ccgtcgcgga tatcgcagag cagaatgaat
     1141 atccggctct ccccatcgac gccggtctcg ttgccgaacg tcccggtaac cgtgggatat
     1201 acgacgcggt cgaaggtctc cacgaggccg cggatctcgc tctctgcaac cgtcaggttc
     1261 tcctcagcaa agagatagca gtgctccccg gttgcctcgc acgtggcgtt gatggtgtaa
     1321 tcttccaggg ttacgaaatc ggtgcagata aacgtcttcg tatcccccgg catgaaggta
     1381 tctgcagcct cctccctcct gccggtatcg tctgtcgcgt tccgcgaggg gtccccccgg
     1441 tcctcgtgga cggcgaggat aaggggggtt ccnnatacag atcacaacca cgaggtccct
     1501 gataccggtc attgttcatc ccaaccttca atggtctcgc ttgctctcca cagtatcctc
     1561 ccggcagccc ggtaccggga gaggtggcgg gcctcgaggc ccgggcccac acccgtcacc
     1621 ggttatcccg tcgtgttggc gggaccagca cccggtcgat gacgtggatg atgccgttac
     1681 tggcgttgat ctccggcgtg ctgaccgtag cgttatcgac cgtcagcaga ccgccgctct
     1741 ccgagaccgt caggttctcc ccggcgagcg tcgtgagagt cgtggtgtta ccaccctctg
     1801 tcatgttcag gagttcggcc gccgtataac tgccgttcac cacatggttc gagaccacct
     1861 gcgtccgtac gttgtcgtcg gtgaggatcc cggcgatgac ttcaggcgtg aggttgccga
     1921 acgcatcgtt agccggcgca aatacggtat acggcccttc attctgcagc gtcgccgcca
     1981 ggtcagtgac gctgatcagg cctgccatga tggagaggtt gccgtcctca ccgatcacct
     2041 cgccgaggtc catctgcccg gcgggagtcg gcatcggtgt cacgttctcc ggtgtggtcg
     2101 ttggtgtggg gatcaaaacc ccgggtattg tcgacgtcgg tgttgcgttc tctgtcactt
     2161 ccggtgttac gttcaccgtc ggcgtcgggg ttacgttccc ctcaccgaac gtaaccgtga
     2221 tcgacagccc tgcaacggcc cggtcggtac gggattcgta acggggatca tagtcggaga
     2281 gatcgcagta gatgaaccag aacgtctcac cgtcggtgag gttgtgatcc cgggcatcgg
     2341 tgtcggtctc gtggaacgtc tcgttgagat cattgagcac gtaccagatc tggtcttcct
     2401 cgtacacgta ttccccaata ctgtcgacga ctaaccggtc gctgaccgtg tagtagtaag
     2461 tcgtgtagtt cagcccttcc gaccgggcaa ccgcatccag cgcgcctatc ggcgtgtccc
     2521 cctccacttc gagggggtcg tcgaggttga tggggtcgac ggtgaagttc tccgtcggat
     2581 cgatggtgac gttatacctc cctatgacct ccatggtatc tatcggcaac tcatactcaa
     2641 gttcatcgcc gattgcgaat cttattgccg ccggatcggc catttcaatg cccggggcgg
     2701 actgcgacgt ctcttctgcc gccgacaccg cgaggacggt cccggtcagg gtaagaagac
     2761 acagaatggt aagaatacta aaaacttttc tcaaagccac ttctatcacc tgtctctgtt
     2821 gcacccggat gctcatgact ggagaagggt tctgatgaac ggtacggtgg aatcgggttc
     2881 aatcggggtt ctgcgtggcg ctgatacagc aatgctggag caccgggctg gttgccggag
     2941 tatttttgtt cgggctatat gactgacaga gcgggtatct ggaagcagcc gggacatata
     3001 taatttttta aataactatt gggccttaca tgagtccgga ggcccctccc cggctggttc
     3061 cgggaagtac gggtgccggg cgagcgtgag caccggcagc ccggaggctg tggagatgcc
     3121 cctccgatcg cacagcacgg cagacacccc cgggaccatc caggtgttga gttgctgcgg
     3181 tgagccaccg ttctggaact tccgaaaaaa aggttattcg ccgaccccgc ggttaccgcg
     3241 ctccggcagc caggtcctcg agggcggcca cacgcttctc gagcggcggg tgcgtggaga
     3301 agagctccat cagggtgttg cccgagagcg ccgggatgat gaagaaggcg tttgcgccct
     3361 ccacttcccg cttcttctcg gcggggatgt agtccatccg cccgctgatc ttcgtgagcg
     3421 ccgaaatcaa cgcccggggg ttgtctgtca gttcggcact gccccggtcc gcggcaaact
     3481 cccggtagcg ggagagagcg cgggtgagca gggtgctcac gatccagacg acgatcgaga
     3541 cgacgtagac caggatcagt gcgcccatgt tgttgttgtc gcggttgccg ccgaagatgc
     3601 tcatgaagag ccagttccgc atgatcagga aggcaagcat cgatatgaaa ctcgccatcg
     3661 tcagggtcag catgtcccgg ttcttcacgt gcgacatctc gtgggcgagc accgcctcaa
     3721 gctcctgggg ggtgagcatc cgcatgatcg agtcggtgac cgccacgacg gcatggctcg
     3781 ggcttctccc ggtcgcaaag gcgttcggga ccggcgaggg catgaccccc accctgggct
     3841 tggggaggcc tgcgccggtg gcgaggcgct cgaccatccg gtgcaactcc gggtactcgt
     3901 cctcttcgac gatccgggtg ccggtactcc agagcaccag cttatcggag aagaaatact
     3961 ggacgaacgc catgccggcg gccatcagca ggaggaactc aaaggggaat ctcagggcgt
     4021 agaggacgcc caggaagatc aggtagacta tgaggagcag gaacgacgtc aaaagcatcc
     4081 tcatcgtgag accgaagtcg cgcgtccact tcatgatagg gatctattgg accggagatc
     4141 tcttaaggta tgcgccggag ttttttgagt gtcaacgttt gaatcgggct ccgggacatt
     4201 acgcgccagc ggccttatgg tatcagggtg agaaagataa agattggatg acactgaacg
     4261 aagtgaccat cagcagggcc attctcgagg agcagcacaa gacgttcatt gactacctcg
     4321 agatggacgc cgccgtcatc ggcgggggcc cctcagggct cgcctgcgcc gcgctcctcg
     4381 gcgagagggg cgtctcgtgc gcactcatcg agaagaagct cagtatcggc ggcggcatgt
     4441 ggggcggcgg catgatgttt ccgcggatcg tcgttcagga agaagcacgg cggctcctcg
     4501 accggtttaa catcgcctgc cgggagttcg aggagggcta ctacgtcgca aaatcggtcg
     4561 aagcggtctc gaagctcacc gcggcagcct gcgacgccgg cgtcgagttt ttcaacctca
     4621 ccaccgtcga ggacgtcatg gtcaggggcg acggcagggt cggcggcctc gtcatcaact
     4681 ggacgccggt cgacatggcc gggctgcatg tcgaccccct caccgtggcc tgcacctgtg
     4741 ccgtcgatgc gaccggtcac gacgccgtga tcgcccggat gatcgagaaa aaaggcggca
     4801 gactccaggt gaagggcgag agtttcatgt gggccgagcg tgccgagtcc cggatcctcg
     4861 atcacacaaa agaggtcttt cccggcctct ttgtcaccgg gatggcggca aacgccgttg
     4921 ccggggagtg ccgcatggga ccgatcttcg gtggaatgct gctctccggg gagcgggccg
     4981 cggaactggt tgcagagaga ctgggccggt aacacttcaa aaccattttt accgccccgg
     5041 tagttaccaa tagtatggaa gagatcaacc cggaatccct gaccgagatc gcatacggaa
     5101 tctttgagat cttcctcaac cgcgaactcc gtctccgggg tccgcacctg tttgaactcg
     5161 tcgaacaggg gaccgatttt gaggctgatc tccgtgaaat cttcgggcgg ttctgtgagg
     5221 actacccgga gctcgccggg gcgctcctcc ggcggttcgg cgggatcgat gcgatctacg
     5281 cacccctcag ggcgggcgaa ggcgtcctcc cctcgaagac gacccggatg tactggcttg
     5341 ttcaggatgc cccggggccc gcggcgagag gcctcgacga cgagcaggtg gggaaatggc
     5401 tcatcttcgt ccccgcagac gaggtggatg aggcgtggag gaaggtccgg gacgagacgg
     5461 tgagaggaga actcgggata tccgcaaaag tcagcaccgc caaaccgaac ccggactcac
     5521 gcgacgagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcaacat cgagacgtac
     5581 aggggcgagt acagcgaaga gggcagaaaa gttacctact acagcgcctg aagaacaggt
     5641 cgccccattc tctgctgtag cgccgaaaaa aaagaatatg ctggatggag cattacgacg
     5701 gcgttacgcc ttccgttccc tggacttggc ccggagttca tccgatccgc annnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgttcttgct
     5821 actattaaca gttctgtcgt cggggtgccg catccggcct cctcctcgat ccaggtacgg
     5881 aacgcggcaa gtgcccgtgc ccggtgtgag aaccggctct tctcggcaag cggcatctcg
     5941 gcgagcgtct gcccggcgtg ggcgaagatc gggtcgtagc caaacccgcc ctctccccgg
     6001 ggggcgacga tcgttcccgg gaggattccc cggaagatcc ggatgccgtc atcccgggca
     6061 aacgcgattg ccgtctcgaa atgggcgctc ctgtcttcca cgccttccat gagtttcagg
     6121 acccctgcgt tcccgatggt gtcgtggacg taggcggcgt atgggccggg aaacccccgg
     6181 agcgcatcga caaaaagggc ggtatcgtcg acgatcaacg ggcgtgagag cgtccgatag
     6241 gcgaactccg ccttcccccg ggcgatctcg ccgacgtcgg cgtggcggaa ctccggacac
     6301 tccagcggga cgtgctcgac ctccagcacc cctgcaaagt aggcggccac ctcccgcgcc
     6361 ttgttggcgt tgctcgtcac caccgcgagc ctcacaggta acgcccccgc agttcgacct
     6421 catgctcccg ggagaggacc tcgtccgcct cccggaatgc ccccgcgtac ccttcgatga
     6481 aggcctcttt taactccgcg aagttctccg tggtgctctc gagcgtctgg aagaagacgt
     6541 ggaggtcgac cccccggctc tcgacctctg atgacgtgga cgcaagtccg aagtcgatta
     6601 agacgcactg gccgtcgcgg acgatcatgt tgctcgtcgt caggtcgccg tgaacgatcc
     6661 ccgccgcgtg cagcctcccc accgcctcgc ccgcgaggcc gatcgtctcg ggggtggcga
     6721 catacttcag cacgtcgccg gcaacgcgct ccataacgat agtgtccgcg gtgacatccc
     6781 ggatcaccgg agtggggacg cccgcccgcc gcgccgccgc aataaggcgg gcctcggccc
     6841 gggtccgctc cgtgatcagc cgccggtcga ggcccgggtt ccggtagcgc ttgctggtgc
     6901 ggtgcttgat tacatccgcc tcccggatcg tcacgaccgc ctccgcaccg cgggcgacgc
     6961 ccccgtcgtg ggcggcggca aagatctcgc cgggctccgt acgccaggcg acctccacct
     7021 catccgcccg aaagcccggt cggatctgtg actcctcgag ggggagcacg acgttgtgct
     7081 ccagcatgat cttccccgta taggcgatca tcgcgccgtt gtcgccgagg aacgtccgct
     7141 ccgggacggc gaacgccgct ccccggtctt cgcacatgac ccgcagcatc tcctgcagcc
     7201 gcccgttcgc gccgaccccg ccgaccagca gcacctcgtc cttgccggca tgcgcgagcg
     7261 cccgttccgt cacctcgacg cacatcgcaa acgcggtctc ctggaggccg aagcagacgt
     7321 cctcgagcgt cgccgcgctc tcctgggcgg cactgacgag cccggagaag gcgaggtcca
     7381 tccccttcac cgtgtaggga agttcgatgt agtccccctg ccgcgcaagg ttctcgatga
     7441 tcgggccgcc cgggtgcggg aggtcgtggc tccgggcgaa cttatcgagt gcgttcccaa
     7501 ggccgatatc cagcgtctct ccaaagatgc ggtaccggcc gttcaagtag ccgagcacct
     7561 gcgtgttggc gccgctcgcg tagaggacga tgggatcggc gaagccggtc gcccacctcc
     7621 cgatctcgac gtgcgccaca cagtggttga cgccgatgag cggcacgcca agggctatag
     7681 agagggcccg tgcggcggtc gccaccgtcc ggagggaggg gccgaggccg ggcccctggg
     7741 agaaggctac ggcccggatc cgttccggct ccgtgagcac ccgggagatg acctccttca
     7801 tcaccgaggc gtgatgctgc gcggcctccc gcgggtggat tcctcccttc ggcgggatgt
     7861 agggcgatga gtggagggcg atcaggtctt ctccgaagag ggcggcactg aggttccacg
     7921 cggttccctc aagacccagc accagcccgt cttcgggcat cacgtcaggc ataaaacagt
     7981 tgtcgctccg ttcgtttcga tcattgttat gaaaaagacc ggccgcggcc agggcctgca
     8041 ggcgggagca ggtgaagcgt ggccgcgacc gtattttgta tccggggtga gaacctacgt
     8101 ctggaactcg gtgtagccgc acttgccgca ggccacgcgg tccttgtgcg cggccatcag
     8161 gacgccgggg ccgcaccggg ggcagtgccg cttctgcaga actgccttgt cgcccttgac
     8221 ttcgtagcac tcgtgtctct tgcccttggc cgactcctgc ttcttggctg ccatgcttta
     8281 cgcctcgctc tccgccctgg gttcgccgcg cttgagcagg tactcccgct cggtcgactt
     8341 cttgctctct tcgtcgtcgt agatccgggc acggccgaaa agttccatct tcccaaaccg
     8401 ggtccgctcg agatccagca cgaggaggtt ctcattcttg ttctgcagcg cagcgagctt
     8461 ctcttggatg ctcttccgcg agggtgtcgg gccatcaaag gtgagcgtaa acgtaagctc
     8521 cctccggttc aacacctcgt tcctcacatc acgggtaatt tcgaagtcca tcgatatccc
     8581 taaaatattg ttgacggatt tatttatact ctgggatggg cgcgggtttt gccgcatccc
     8641 tctctcacgc acgaatacgt tcagcataga cgcggcctcc tgcttcgccg gtgcgtccac
     8701 gagccggagg acgacccctt ctcgcggctg gccgtagagg atggcagccc cgggtggcgc
     8761 ggcgaggacg agcgggatga ccgcgagatc ctcctcgccg tcgacgaaga tcacccccgg
     8821 gggatttttg accacctcat cgatcgcatc cacgagttcg tcggtgattg tgccgggagg
     8881 gttcttcgct gtcagccttc ttgccttgag caggggcgaa cgggtgcagg gtatgcgcat
     8941 cgtgtagccg tcgatgatgg cgatatccgg gacgatcccc gcatccagga ggttgtgggt
     9001 caccacatcg ccgacggcgt agaccgccct gccttcgagc cgggggagga gttcgccgat
     9061 acttccgtaa aggctgccga acggcttttt gaagagatcc cggtgcgctt cgggaagccg
     9121 gagcatcaac ggaccttcag tgcgtacctg cccgccatcg tgatgttcat cttcttcgcg
     9181 atctccgaat gctcgggatc gatgatcacg acgtagccag cccagtcttc gctcaggttg
     9241 gccgtaccgc agatgacgca gttctcccct tccacgaccc ggtggcactc gcggcagacc
     9301 ttgaccacct tcttacggac gaccatctta cgccgctccc ttctccttct cgtgttcgag
     9361 ttcctcttcc agccagcccg tggtcccgag gcccgactgc cgcatggtga ggccgatctt
     9421 gctctcccgg ggttcacgct cgttcaacga gagtgcaaca atccgggccc ggatgccgtc
     9481 gccgacagta atggcccgct ttgagtcctg acagatcagc ctgccgttct tctcgtcgta
     9541 atttatgtat tcatccgaga tctggctcac gtggagcatg gcgtcgatgg gcccgaggct
     9601 gatgaaggcg ccgaaactcg tcgtctccac cacctcgccc tcgatcactt cctggagcgc
     9661 cagacggagc accgccgcct cgaatctgac atcgtagtag acggcaccgt ctccggggat
     9721 caactccccc tcgccaacgg tgagaatctt tgtaaccgca atgaagatgc cgatctcctt
     9781 tgcgatgctg ccttccagct gttcctgcag tacgttgagg atgaccttct ccaggtcttc
     9841 cccaaggcgg tgcggcggaa cccgcacctt gtcctccagc gtcatcttat aatacatggt
     9901 ttcactatcc cctcatcagc tccaatgttc tctctctccg catcgacaca acgtcgattc
     9961 cctcgccgag gagagccttt cggagctggc gatcattcgt caccacggtg catccttccc
    10021 gtcgggcata ctcgatcacc cggtcgtcca cacgctccgc ggtactcccg ctcggtacga
    10081 ccgtcgagcg ccgggccatc gcgaggccca cacgggcggc agccgcatca cggccgcggc
    10141 cccgggaaag tccggagagc tcaccgacca cctcttcaag tgttatcggt tcgaagtccc
    10201 cgaagagggc caggagctca tcatacaggt caaccctgaa ctgggctggc attaagaggg
    10261 cgttcgtgtc gaggagcacc ctcactcgac cagaatcccc atgccgatca gccgccatcg
    10321 tccgccgacc tgcctgctga tggcgatccg tgcgccgact tccgcgcaaa ccgcccgctt
    10381 cagctggacc tctacctggt tcttcttggt gttcgtgatc acgccgacgg tgacggcggt
    10441 gccgaccgag agcatcagcg gctctttatg cttcaagggc tcgatgatct gctcgctgtc
    10501 cgcgccgacc acccgttcca tgagtgtgac atcgaacttc agccgttccc agaccggcgg
    10561 gagttttccc acgagtccgg caacctgccc ggcgagggca tcgctcttcg tcagtgcagg
    10621 atcgagtttc gtcccgaccc cgaggaggcc cccgggcgcc gcctcggtca cactggtatt
    10681 accggcgttg atggaggtga tcttcgtctc gaccggctcc cacttcgtcc ggttctcaac
    10741 ctggacctgc cgacccggtc ggatctcgat atcgtcgccc tcatgcagga ctcctctgat
    10801 gagcgagccc ccgatgacgc cgcctttcac gtcccgccag ctgcagccgg gtttgttgac
    10861 atcgaacgat cgcgccacga gcagcaccgg gtcaacatcg gggttgcggg tgggctccgg
    10921 gatgacctcg tcgagggtcc ggatcagggc accgatattg acacctttct gtgccgagat
    10981 cgggatgatg ggcgcgttct cggcgatggt ccccttgatg aaccgtttga tctgcttata
    11041 gtgctccagt gccacggcct ggctgaccac gtcgatcttg ttctggacga tgacgatctt
    11101 cttgatgccg atcagctcga gcgccatcag gtgctccttg gtctggggct gcgggcagac
    11161 ctcgtttgca gcgataacga gcatcgcgcc gtccatcagc gcggagccgg agagcatcgt
    11221 cgccatcagc gtctcgtggc cgggggcgtc gacgaacgag accgttcgga acgggactgc
    11281 cttttttccg cagatcgggc attccgggtg ggaagtatag gcctccgccc cctcgcactc
    11341 ctcacatttg taaaaagttg tatccgcgta gccaagccgg atggagatgc cgcgcttgat
    11401 ctcctcgctg tgcctgtccg tccaggtgcc ggtcagcgcg ctgaccaggg tggtcttgcc
    11461 gtgatcgaca tgacccacga gcccgatatt gacgccggga atgaatgcat ctcgcaaaag
    11521 ttttcgaacc tccttatcca atatatagtg acactactta tacataatcg gtagggatta
    11581 cgtttctgtt ggcggccgct ttttccagtg ccccgggcga tctcacgtct ggcacggttc
    11641 gtgcggcata cgaagtatga ataatgcccc cgattgttag cgaaaattta taccccatga
    11701 atagagatat gggaactgat cgcaacatgt caggtgatac cgaactttca cgtatcggga
    11761 tctctttacc caagaacctt ctcgataaat ttgacgagat cctgaccctc cggggatact
    11821 cctcccgatc cgaagggata cgggattcaa tacggagtta catcacccac tatcagtgga
    11881 tctccgatgt aaaaggagag cgtcagggtg tcgtcaccat ggtatatgac cacgaccagc
    11941 gggggctgct ccagacgctc accgagatac agcaccagta cgcccataac atccaggcat
    12001 cccttcattc gcacgtgacc cagaacaagt gccttgaagt gatcctgatc cggggggacg
    12061 gagccgtact gaaggatatt accgagcggc tgatggcaca gaagggcgtt gaggcggtga
    12121 aactcaccac cataccgatc gagggttaac cgccaccctc gcagctctga tcggtcccct
    12181 tctcccacac ctcttcgagc tgctctttaa gttccttatg ctcttcgagg tagtcctcga
    12241 gagagcgctc cttcctctcc acgttcgcga ccacccggtc ggcactgatg agttcgtcca
    12301 cccggtagta gaggccggtg ctgtcgagtt cggcgtactt ccagccgtcg atctcctcga
    12361 cccgcacgac cttcccggtc gtgccggtgc gggggtatcg gacgactgcg cccactgcta
    12421 cgtcatccgt gctcataacg tggtacggta catctcgcca tactattaag gtatcacctc
    12481 caagggatct tgaatggcaa tcacgattcg tccggtagag aagctgtact ttgacggaac
    12541 gcaccgctcc cggtctccgg aggagacccg tgccgccgtg gagccgttga tggcggagat
    12601 cggtgttacc gagatcatcg atgtgacccc cctggatcgt ctcggtatcc ccgtcttctc
    12661 ggcggttcgg ccgggagcag cccggggagg ggttcgcgtc cacgcaggaa agggaaagga
    12721 accgatccat gcccgggtat cggcgatgat ggaggcgctc gagcgttact gcgccgagta
    12781 ccggggcgac cggatggagt atgcgaccta cgaggagatc ggcccgggac gggcgatgca
    12841 tcccgaagac ctgctcctcc cgcgaaaact cgagcaggga gagaaggtcc actggacgcc
    12901 gacgtgggat atcttaaacg atgaggaggt ctatgttcca agcaacgccg tcttccaccc
    12961 ctacgactca ctcggcatgg ccgtgcctct cttccggagc gactcgaacg ggcttgcatc
    13021 gggaaacgtc atcgaggagg ccattctgca cgccctcttt gaggtgattg agcgggacgc
    13081 tctcagcatt gccgaccagg agcacgacct cggccgccgc ctcaccattg ggacgggatg
    13141 cgccgcccgg gagatcctcg accggttcga ggagaacggg atcgatatcc acctctggct
    13201 gctcgccggg aagaccggca tccccacggt cgcggccgcc gcggacgaca ccgtcacgaa
    13261 ggacccggcg atgcttgtca tcggatcggg gacgcacacc tgtcccgaga tcgcagcgct
    13321 ccgcgccctg accgaggtgg cccagagcag ggggagttat ctccagggcg gccgcaatga
    13381 cccccagcgg gagatgataa tcagaaaggc gggctacgat cggctgaagc ggatcaacag
    13441 gatgtggttt gccgatgcag atgccgttga tatcagggac gtccccgatc tgagcacccg
    13501 gcggttcgac ctcgatatag ggcgggtgct tgaggaagtc agaccctata cggaccgggt
    13561 ctgcgtctgc gacctctcaa ggaccccggt gccggtggtt cgggctatca tccccgggtt
    13621 tgagatatcc tatatggacc cggaccggaa gcgctccgtg caggattgag gctctccacg
    13681 ggctagcaga accggacctt ctttaagatc tccacgaccc tttccgtgct ccggtgcagg
    13741 ggcctttcga cgggcacgac ccgcttcccc gagagggcac tgaatatctc gccgatagcc
    13801 tttccgagcg acggcccgta gccgccctcg agtgtcagtg cgagggggag gtccgtgccg
    13861 tcgatcagca tcccggcgag cacgccgtag tcctcgggtt caaggttcat ccccccgtgt
    13921 tcgtcgtcgg agagagcgtc ctggccggcc gagatgatca gggcgtcggg ccggaaccgg
    13981 gtgagtgccg ggatgaagac ctcgtcaaaa acatactggt agtcggcgat ggtgcaccct
    14041 ggcgcaagcg gggcgttgag ggtgtagccc cggcccgcgc cggtgccgat ctcatccaac
    14101 cacccggtct tcgggaaact gttctcctgg tgcaccgagc agtagagcac ccggtcgctc
    14161 ccgtaaaatg ccgtctgggt gccgttgccg tggtgcaggt cccagtcgag gatggcaacc
    14221 cggtcgaccg actcgagggc tttcgccgcc gcgacggcgg cgttattaaa gatgcagaac
    14281 cccatcgccc ggtcgggctc ggcatggtgg cccggggggc ggaccagggc aaacaggtgt
    14341 tcgccgtcga gcgcccgctc aaccgccgca aaggtcgacc cggcggcata cagcgccgct
    14401 tcgaacgagt ggcaggtgac gtaggtgttc gtgtctaaaa aacaggttcc ccgggcgagc
    14461 tcccggaccc acctgatgta ttctgggcgg tgaacccgct caagatcgct ctcggtggct
    14521 cgaaccggag cacgccacct cacgttctca gggaccccgg agagggctac ccgcaaccgg
    14581 gagcccgact cggggtgagc ctccatgtcg tgcctggtaa agacgtcacc atagatgact
    14641 gaagagcgca tcctcgttca gttaatgatg cgtccctgct ggatatcctc ttcggtgacg
    14701 ccgaacgtct ggccggtgcc cgcaatccgg tactctcccg tcgccttcac ctcacccgac
    14761 catctggatg tcgcgtacgg gacgacgaac gtgccgttca cgctctcctg ccggtaggtg
    14821 aatgtccggc cggtgttcgt cgtgaccggc acctcgatga tcccttcgcc gctgatccgg
    14881 gccccgggga cgtactcgaa caccttgacg gccttcagat caggcccgct ccccccggtg
    14941 ctgccgtaga cgttctgggg cgtctcgtgg acgagccggt agtgctggag cgccgggacc
    15001 cgctcgatcg gctggaggat cgagtccccc cggtactggt aatacatgtt cagcagcgtc
    15061 gcacccgacc ctgccggcgc attcttgttg taggcctcca ccgcggccgc gccttccgtg
    15121 gcgttcatct gctcggtgcg cgtgatgacc gggagagagg tgttcgccgc gctctcctca
    15181 cggtactcgg catagatcac ctgcgacgtc gggtcggtca tggacccgtc gaagttatgg
    15241 agccgggaga tcatcgtcag gtaatactgc tggctgtaga acggcatcga ctggtagttc
    15301 gtcgatcccg cgctcgccgg gaggaggaag tagggctgga accgctccac ccccacatca
    15361 gggtcggccc acgtcgccgg ggcgtggaac ttcccggtat cgagctggat atcggtgacg
    15421 acgtagcggg tgccgatgtt atcgaggatc tggtttgcgg ccccttcaga ggtcgagacg
    15481 aagtacgttg ccgatccgtt cgggccggca accccgtgct ggaaggggtt gttgttcggg
    15541 atccgcttcg caacgaaggt gatccagtgg ccgtagtccc accaggacat gacgccgtag
    15601 gactcttcgg ggtaggtgaa ggtgttccgg tcgtagatgg cgtagtagtc gacgccgggg
    15661 tcgggggtgt tttcgcccat ccatgccagg gcatccatcc actgcgagtc gatgccgttg
    15721 tatttggcac tggcggccat ggcgaggttc gcctggaggg aggctcctgc gaagaggagc
    15781 gtgacgatga tgaccgcggc gaaggctccg actttgaggt agtccggctg gttcctggag
    15841 gagactttcg acttgtggga atccggggcc tttcctccct tcttcccttt tcgtggcggc
    15901 tcctccaccg gcccggggcc ggaagaagtc ctgtcgctcc ccgaccggaa gaggcgggtg
    15961 acgtccttgt acccggcgtc gaggaccgct cctgcaaaga ccgccgagag cagggcaatg
    16021 ttcgcggcga gataatattc gtaccggaca tgcgccatcg tcgaagcgag gatgactgcc
    16081 gtccagatga ggacgaagac gtgggccggg ttcacccgct cacggctccg ccagagcagg
    16141 gtggcaaccc cgccggccat gaggaggagg ccccagtgga atgtctgcca ggccgcgtcg
    16201 aacgaccagg cccgcgcctc ctggacggtc gtggtgaccg cctgctcgcc gaagaaggca
    16261 aagaggctcg atatcaggag gttgtagacg tccggcaggg caacgaagag caccgcgacg
    16321 gcgacggccg cgagacccgc gagcgcggcc gggaagtagt acttcgggcg tcccttcagg
    16381 tagaccgaga ccccgtagag cgccagtgtc ccggcaatga ggccggcata tgcgatgacg
    16441 tggccgatgg tgtaccggga gaggtcgagg ccggcgtgcg gaaagccgaa cgccgccatc
    16501 ccgacgatca ccacgccgaa ggtgacgacg tttacgagaa ccagatagtc gctcgtccgg
    16561 tcctggaaga agtccaggag gaactggacc agggtgaacc ccgccacgat gagcgcaaag
    16621 aggatcatcg tcggcatgtt gaagaagccg aggaggtaag cgatcccggc gagcgccgag
    16681 gcgagcaccg gtgccttcag cgtctcgata ttcagggtgt cgcggctcct cagggagagc
    16741 gggcggtcgc ggacgacgag cagcgctgcg atataggcaa ggacgaagat cgtcccaaag
    16801 agggtctccg cgatatgatg gtcgacgaag ccgaagaggg agcggtaggc gtagttcccg
    16861 gagacgacca tgatgaggcc tgccgcaatg aggccggtct tccagtcggc gatctttttc
    16921 gcaagcagat aggtgaccgg caccatcgcc gcggccatca gcggcgggac ccatgatgcc
    16981 accaccatga tctcgggccg cgtcgttgcg ccgacgagga cacagagcgc cgatatgatc
    17041 tggatgaaga gcggtcccca gtagatgacg tcaccgttcg ggtagagcgt catggtgtcg
    17101 aaccaggcgt agcccgggaa gttggcgacg gtctgctcga cctgccggag actgtaccag
    17161 gggtcgttgc ccaggagatt gacgccttct gcggtcacta tatctgccgc agggattccc
    17221 cgcgtccaga gcgtgagcag ggcgaacagg acgataacac caatgatgat atagggttgg
    17281 tactttttga gatccatctg agccatcgga cactccttgt tacgaatctg tttcctttcg
    17341 aaccattaat aatcttggcg tgtccggaag atccgaaaat agaagcgttt attggggttt
    17401 tcctgttaat cgggcaaaga ccttctcaaa cgcctctgcc ttcttcttcc agctgtgctc
    17461 ttcgatcccg accgggcacc gccccgggga gagagccagt tcatcgaccc gctccaggaa
    17521 ctcttccctg ttccggtaga ccgtgatgtg gtcgccgccc atccggagca tatcggggat
    17581 gggggtggag aggatcggtt tcccgcatgc cgagtactcg aagtacttgt tcgggagggc
    17641 gatatcgacc cactggggag gagagagcgg gatgaggcag aggtccatcg gtgcgatgta
    17701 gccggggagg tcacggtaat cgacggtgcc ggtgaagtgc acccggtcgg caactccgag
    17761 ttgggccgcg agggtgcgga ggtcgtctaa gtagccggtg aagagcgaac cgccgacgat
    17821 caggagttca gcgttagggt gcttttgcag aaccttcggg aacgcccgga tcacctcgtc
    17881 gagggcgtac caccgctcga cggcaccggc aaacccgaga acgaaggcgt cgggagcgat
    17941 cccgagggcc gagcgcatcg cattgccgtc catggggcgg aagagatctg tgttgacgcc
    18001 gttcgtgatc aactccgcct cgtagccgta ccctttgagt ttcgtaacga gccccggcga
    18061 gaccgtcgtg atgacgtcgc tgtggtccag gttgtaccgg gttatggcga ggacgccttc
    18121 ccggagcagc cattgcgcgg cggggttcct gtagtaggcg gctgcggagt cggggaacca
    18181 gtctttgagg tcgaagatca ccggcacgtc gaacttctgt gctgcccgga tcatcgccgt
    18241 ccccgcgagg acgtgggcac cgacgacgat atcgatatcg tagtcccgga tgatgtcgcg
    18301 gatgacccgg tagtggcagg gtgcgtttaa ggtgtagtgg aggaaggggc tctccaccgg
    18361 aaattgcgtc gcctcgtgga cgtgaagaag ggtctcgcgt tcctttcccc tgctgacgtg
    18421 gaagtgaggc acgtggacct cgtgtcgtgt ggccagttcc tcgaagatgt tgtggtgcct
    18481 cgacgggatc gggtgatgga tgtagtcctg ggtggagacg aggaggattt tcatgagtat
    18541 ctaaaaatct ggttattcgg cccggagtga ccggaccaca tcgcagatat atgccagttc
    18601 ttcgtccgtc accgacgggt ggacgggcag gctcacgacc gaggccgcga ggccatccga
    18661 taccgggcag gaagcgtgct cgttttcgta taccggctgc cggttgacgg ggatggggta
    18721 gtggacggcg gtgccgatcc ccttatcgtc gagagcgctc atgaacgcgt cccggccgag
    18781 cgggtagccg gcggggaggg ttgctacgta ctggtggtag acgtgccgga cgtccggtgc
    18841 tgcgtagggc gtcgcgagcc ccgtgcccgc gaggtggcgg tcgaggtacc gggcgttgtg
    18901 gatccggcgc tcgttgaacc cctcgacccg gtcgagctgt acgaggccga tggcggcggc
    18961 gatgttcgtc atccggtagt tgtagccgac ggccgagtga aggtacttct tgctctgccc
    19021 gtggttgatg aggagcctga cccgttctgc aagggcatcg tcgtcggtcg tgaccatgcc
    19081 gccttcgccg gtggtcatgt tctttgtcgg gtagaacgag aagcaggcaa ggtcggcaag
    19141 gcctcccacc cgcttccccc ggtactcggc gccgtgggcc tgggccgcat cctcgatgag
    19201 gacgaggtcg cggtcgtcgc agagagcgcg gatggcagcg acatcgaacg gctgcccgaa
    19261 gaggtggacc ccgatgaccg cccgggtatc cgggcggatg agaccctcga ccgagtccgg
    19321 gtcgatgttg aacgtccgtg gctcgacgtc cgcaaagagc ggcgtcgccc cgcacatcga
    19381 gacgctcgag gctgtcgcga agaacgtgaa cgccgggacg atcaccgagt ctcccggccc
    19441 gatgccggct gcaaggaggg ctgcgtgaag tgccgccgtg ccggagttga cgccgactgc
    19501 gtgcgaaaca ctgcagtaat ccgcgaaact ggattcgaac tcagtgacga ccgacccctg
    19561 ggccagcatc cccgaccgca tcacactggc tacggccgcg atctcctcct ccccgacgat
    19621 cggccgggcg acggggatct tcaccggcac cgcctcgcct ccaccgggag cggccggaac
    19681 cgtgccggcg tcccgaccgc aagtgtcccg ggcgggacgt ccttcgtcac caccgcaccg
    19741 gcggcgacga acgcgccggc gccgacagtg acgccgggaa ggatcgttgc gttcgcaccg
    19801 atgacggcat cgttcccgat caccgggccc cggagggatt cgagggggcc gggagggtag
    19861 cggtcgtttg tcagcacggc gttcggaccg atgaagaccc ggtcgccgat ccgggcgccg
    19921 gtggggatgt agaccaggct ctggaggcgg acatcgtcgc cgatcgtgca gtcgccctcg
    19981 atcaccgccg ccgtcccgat cgctacgcgg tcgccgatgg tcgtcttctc gcggatcagc
    20041 acgttgtggc cggtctgaaa gttctcaccg atcgtcacgt cgcagtagat gatggtgccc
    20101 gaccggagga ccgcgttcct cccgatggtg gcgcccgggt attccttctc tcctatccgg
    20161 tcacgggagg gaaacccgat ggtcaccggc tcaaatatca ctgccccctc accgagggcg
    20221 ttacgtccat gctcaatcat tcaaccgtca cgctctttgc cagattccgc ggcttatcga
    20281 cgtctcgctg cagcgcacag gccgtatggt aggcaagcag ctggagggcg accgacgcgg
    20341 tcaggacctg caccagaagg tgtgtgttcg ggatggggat gaagatgtcc acgatctcag
    20401 cgagttccgt atccccggca acgccgagag cgatgaccgg tgccttcctg gccttcatct
    20461 ccttgatgtt cgaggccatc acgccgtagg tcggcccggg ggtgcagatg gcgaccaccg
    20521 gtgtctcggg ggtgagcagg gcaaacggcc cgtgcttcag ttctcccgcc gcatacccct
    20581 cggcgtggac gtaactgatc tctttcatct tgagggcgcc ttccatggag accgggtaaa
    20641 acgccccccg tcccacgaag aagacgtgct cggccttcga gcagagagtg accgcctcgt
    20701 ggacgtcctg gaggaggata tctcctatgg ccaggtgcgc gtgcgacagg atgtcgtcga
    20761 acgccccctc gcatcgcagg ttgacgatct gcatcagcgc cgcaagctgt gccatgtagg
    20821 acttggttgc ggcgacgcca atctcggggc cggcccgcat caagagggtc tcatccgcaa
    20881 cccgggtgac cgtgctcccc tgcatgttgg tgatggcaag cgttgggcag ttgcgggctt
    20941 tcgccatctt taaggcggcg atcgtatccg cggtctcccc cgactgcgag accgcgatca
    21001 cgaggccgtg gaggggtggg gtgaagtact tgaactccga ccctatctcg acccgtaccg
    21061 gtatgttgca gagcgattcg gccaggtact tgaagatcag ggcggtatgg tacgacgtcc
    21121 cgcatgcgac cagggtgatc ccgtctgcct ccatgaccat ctgccgtacg cgatcgtcga
    21181 tgccggccct gatggtctcg tagaaagatt ggggctgttc gtagatctcc ttcagcatat
    21241 agtgggaaaa cccgcctttc cgggtatctt cgacgcacca gctgatcagt tcgaccgggc
    21301 gctccacctt ccggccgccg tggtagatag cgaagtggtc gggggttagg tcggcgacgt
    21361 cgccgtcctc aaggaagatc acgcgttcag tgtactcgag gagcggcgtc atatccgagg
    21421 cggcaaagac ttcggcatcc ccgacgccga ggacgagcgg gctcgcgtca cgggcggcga
    21481 cgattctctg cgtatcctcc gcgatcgcga ggacggcgta tgagcctttg agcaggggca
    21541 gggtcgcgtt gaccgccgcg aggagatccc cgtcatagtg ctcctcgatg agatgggcga
    21601 tcacctcggt atcggtctcg gaccggaagg tgtgaccgcg cccctgcagc tgccgtttca
    21661 gttcgctgta gttctcgata accccgttgt gcaccacggc gatcttatcc ctgcagtcgg
    21721 tgtgcgggtg ggcgttgatg tcgttgggtt cgccgtgcgt ggcccaccgg gtatgcccga
    21781 tcccggtaca cccgtggagg ccgaccactt cggcctcccc gtccgagatc cggccggtct
    21841 tcttatagac ctcgatcgca ctcccgatcg tcgcgatccc gaacgagtcg tacccccggt
    21901 attcaagccg tttcagcccc tggatcagga ccggcgttgc gtcccgtctt ccgatgtagc
    21961 cgatgatccc gcacatctac atcacccgcg tgccatcttc gaggaggccg accactgtct
    22021 tcccctcctc aatagtgaca ctgttacctg ctatgcagtt tttaaatgtt gtgaatggtg
    22081 ccgcacggac cccgtcaccg aggactgcac cgaattcctc cttgtggacc cggcccccga
    22141 cctcgatgaa gctcgctgaa gggtaggtgg tggtatggtc ggcaaggatg cacccctgac
    22201 cgaagacggc agagacaatc ctcgaatggg acccgatcgt gacgtcgctc atgatgagcg
    22261 agttggcgac gtaggtgaac ggctcaagca ctacccggtc gccgatgctc acatcgggca
    22321 tgatcacgca gttcgggccg atgttgcagt tgctcccgat ggtgacgggg ccgtagatga
    22381 cggtgttcgg gcccacggtc gtcccggtgc cgatatgcac ggtgccccgg cggatgacgg
    22441 atgcatcgac cgtcccggcg atctcggggg tgatcccttt gagcatccgg ctgttcaatt
    22501 tcaggaggtc ccagggatag atggcatcct gccagtcatc tgccgggatc gcccgtatcc
    22561 tccggcccga cgcgatcatg gcggcgagag cgtcggggat ctcggtcgtc cggaggaagg
    22621 agaagacgtc gggtgtgaac gagtagaccc cggtcgatac ggtgaacgtc ggggcgtcct
    22681 cgggtttctc gatgacctcg cggacgaggc cgtttctgat caccacgacc ccgaagttcg
    22741 aggggttcgg gtgctcggcc acgagcatgg cgttctgctc ctttttaatc cgggcaatag
    22801 attctgcgtt gatgtagttg tcgccgggga gaacgaggaa atcgtccgtg atctccgact
    22861 cggccgcacg gagagcatcc gcggtcccga gttgccgttc ctgcacgacg acctggatcg
    22921 gggcgtcgag ccggttgagg tgccggatga catcctcttt acggtatccg accaccacca
    22981 cgatatcgcg gatcccgttc tccagcaggg cgtcgatgac gtattcgatg atcggccggt
    23041 ttgctaccgg aagcatggct ttgggcttgc tccgggtgag tggccggagg cgactccctt
    23101 ctcccgctgc tagaatgact gcctgcatca tatcacctga tgattgaacc gtcctcgatg
    23161 catccttcga cgagggagtg gggagcaatc ctcgcgttac tgccgatgag gctcccgacg
    23221 ttgaccgagc agttgatccc gaagaggacg tcatcgccga tgatggcgcc aaatttcctc
    23281 cgcccggtag tcttcccgca gaccttcacc gatccattgt catgcctgag gtttgcgacc
    23341 ttggtgcccg ccccgaagtt gcacccgctc ccgacgatgc tgtcgcctat gtagttgaag
    23401 tgggggatct tcgtcgccga catgatgatg gagttcttga tctcggtcgc atgcccgatg
    23461 tggcagccat cgccgatggt ggtagagccc cggatatagg cgtgggggcc gatgatacag
    23521 ttctcgccga tgacgcagga accctcgatg taggtgccgg cccggatgac ggtccctttg
    23581 ccgatgctga ccgtctccgg gacggtgcac ccgtcttcga cggtgccgca ccgttcatgg
    23641 cagagcgagg agaggagttc ctcgtttgcg tcgaggagat cccatggctg cccgacgtcc
    23701 agccagtagt ccagcgggta tgctctgagg gttccctccc tgatataaga ttcgagggcg
    23761 tcggtcagtt cgaactcgcc ccggcccgat atcgtgagcc ccgcaagaag gtcgaagatg
    23821 tcggggtcga agaggtaggc gcccgcgttg atcaggttgc tttttggagc ttcggacttc
    23881 tcctcaagac cggtgatgcg ccctccttcc acggtcacca cgccgtagtc ctgcgggtgg
    23941 tcggtctcgt ggatccccac gcagggggta tccatccggc agaactccct gatgtcctcc
    24001 tttttgagga ccatgtcgcc gtttaagagg aggaatcggc cgtcaatcat tcctgccgta
    24061 gcccgcaggg cgtcggcggt gccgagctgg tgccgctggg tcacgtacgt gatattcacg
    24121 ccgagaacgc tgccgtcccc gaagtggttc cggatctcac gctcaaagta gccgacgacg
    24181 aagatgaagt cggttatccc tgcgtcgtga gccgcgagga cgaggtgttc catcatcggg
    24241 cggttggcta tggggagcat caccttcggg cgccgggcgg tcaggggacg catccgtttc
    24301 ccctctcctg ctgccagtac aacacattgc atatattcac tccaggatat gtttggcttg
    24361 ctcaagggct gcgtgcccca tatcgagcat ctcttttgct tttgcgggcg tctttccctc
    24421 ggccgtgatc cggacctttg gttcggtgcc gcttgcccgg atcaggtacc aaccgtcttc
    24481 atcttcgaac cggataccgt gtgtcggctc atcggcgccg atcgcagcca tgatctccgc
    24541 cggagcgtcg acgcgatacg cctcccggag gagtgtgtac cggggcatcc ggtcaagttc
    24601 ttcggcgatt gaccactccg acgcgatctc gcagagcagg gctgcggcgt agatcccgtc
    24661 cgggcagtag gagtgctccg gaaagatcca gctccccgat gcctcgccgc cgaagtcccc
    24721 ccaggagagg agctcctcgg agacgaaact gtcgccgacc ggcgtgcgat ggacctccgc
    24781 aacctcttcg atcgccatgg aggcatcgac ggtggtgacc acgtgcttcc ggtcgaggta
    24841 cttcgcgaag agcatgagga gatggtctcc gtcaatgtag cggccccgct cgtcgaacgc
    24901 catcatccgg tcggcatcgc cgtcattgac caccgcacac gcggcctccc gcttccggac
    24961 gatctccccg atataaggga gatttgtctc gagcggctct gacgggcggg caaacttccc
    25021 cgagacgttg cagttcaggc cgatgacggt tgctcccatc cctgcaagaa gatcgggggt
    25081 gatgacgctt cctgcgccgt tgccgcagtc caccacgacc gtgacgggcc tctccaggct
    25141 gacgcggtcg aggatcgcct cgcggtgcgc gtccaccgca tcgatgggag agacggttcc
    25201 ctgctcgtcc cagttcttcc agtgcgaccc ctcgatcgca tcctcgatcg ccgcctgctg
    25261 ccggagggtg aacgacgacc cgtcggggtt gagcagcttc accccgttgt aggactcggg
    25321 gttatgcgag gcggtgatca tgcaccctgc gtccacgcat cccgtcgcat gggcaaccgt
    25381 cggcgttggc gcgacgccgc aggcgtagac ggttgcaccg gccgagagca tcccggcaat
    25441 gacactatgt tccagcagtt cgctggtcgt gcgggtatcc ctgcccacga cgatgcccga
    25501 tgcacccccc gcggtggctg ctccgatgcg gagcgccaga ctcacgaggg cggggccgaa
    25561 ttctcgccgg attccggaag agccgaaaag catactcctg tataatcctt tctttcctaa
    25621 aaaaaggtat tcatcgcccg taagggcacc agtgcttgag atttaaaaga ccgctgaccc
    25681 cggcggttga cggcccgagg aagatcatct gcttgtgtcc ccggtactca tcgatgcagg
    25741 caacccccgc gtccgtcgtc atgccatcgc cggcgaccag aaagaggtct gccgcctccg
    25801 gcgaatcgac gatctggctg ccgagggccc gctcgagcac cggcatcggg ccaacgaggt
    25861 agatccgttt tccggcgacc tcgcgggaga gttccgcgag gcagggggca tagcggtcgg
    25921 gggtgcaggg gtggagtttt cgtgtcaggt agaggaacgc cgagacagcg ttgatgatcg
    25981 cacctgccgc cccccgctgc tttgggtttg caagcggcgc gccgaacata aaggagatcc
    26041 gggtgttggc ccggacaggt tcacgggtga tgaactgccc ggtctgcccg ccgaagtgcc
    26101 cctccatgca gacccctttt ggatactggc agacgtctgt atcgtggttc acacagagct
    26161 tcacgattcc gtcctcgcac ccgctgtccg cgatgatcgt ctccagtctc ctgattgcat
    26221 cgtgccagaa actcatgatt tttcctctgt atcccacagg atcgcccgca cgggcgaccc
    26281 gtccgcacct ttgagccgca tcgggagcgc caccatgaag tagtttccct cgggcacggc
    26341 ggagaggtcg agcagttcaa ggatgaccat cccgctcccg agcagccggc ggtggaccgc
    26401 accgtcgcca tggaatgcct cgatcgacgg agcatcggtt ccgaggcagg ttaccccggc
    26461 atcagcgatg aggcctgctg cctcaacaga gagcgccggg tagccggggt cgaactgctg
    26521 ccgctccgag aaagtggtcc ggaggaggag cgtcctcgcg ccgtcgaggc ggccggcgag
    26581 gtggtcggga ccaatgacct ctcccgcgtc gctgcagtcc agtacctgcg ccggtcctgc
    26641 gagcaccccg ggcgggatcc gatcgaccgt ctgcccgccc ttgatatagt gcgacggggc
    26701 gtcgatatgc gtcccggaat ggctcccgag ggtcatctcg gtcacccggt actgcccgtt
    26761 atctatctcg cggaaccggg gccggacgtc cccggggtag aggatcgcat cctcggagag
    26821 gtcccgggtg atgtcgtaag tcttcacagg taatcttctc cccgccagcc gaactgcccg
    26881 atcagcggca cgaagcagac gccgccgagc gggatcttct ccaccctgcc ttcgtgtttg
    26941 acgagtttga tgaggtcctg gcattcccgc ggcccgacgg gagcgatcag ccgcccgccc
    27001 tcggcaagct ggtcgatcag gggcccgggg atggtgggcg atgccgccgt gacgacgatg
    27061 gcgtcgtacg gcgcttccgg gggatagccc tgtgtgccgt cgccgacgac cacccggaca
    27121 ccggtgaccc ccgcccggtc gaagttctgt tgtgcctggt cggcgaggtc ttcaaggcgt
    27181 tcgatggaaa tgactgttgc ggcgagttcg gcaaggagtg cggcctggta accgctcccg
    27241 gtccctacct cgagcacccg gtcctgcggc gtaagttcga gctgttcggt catgacggcg
    27301 acgatatagg gctgggagat ggtctgcccc tccccgatgg ggaggggtcg gtcctcgtag
    27361 gcggcacgct catagccctt cggcacgaag aggtggcggg ggatcttccg catcgcggca
    27421 agcaccctct gatccccgac cccccgggcc cggatctgga actccaccat ctcctcgcgc
    27481 tccccctggc agggatcggt ctgtgtcatc ccctctcccg aatcagaacc ctgattctgc
    27541 tttatcgatc gcggcatcga tctgcgcctg caggaccgca ggcagaccgc tgatctttac
    27601 atccaggaaa ccgcgtataa tggttgcggt ggcctcctcc tcggagagcc ctcttgccat
    27661 gaggtactcg atctcgtggt gggcaatctt ccctacagca gcctcgtggg agagttcggt
    27721 cccggtcagg gttccttcga tctcggggat ggcgtggatg atgccgtctt ttaagatcag
    27781 gcccttgcac tcgatgtggc cccgggtgtt attcttctcg ccgaggatgt ggccccggga
    27841 gatgatggtg ccgccggtgg tgatcgcccg ggtgatcagt tcggcgctcg tatgctcggc
    27901 gccgaggatg gcgcgggagc cgaggtcgag ggttgagccc ggtgtcgcga cgacgatgct
    27961 cgagaaccgg gccacggcgt tcttcccgac caggcgtgcc gtcgggtaca tgttgacctg
    28021 gtgaaccggc tgcatgcaga cgtagttcga gaggaaggtg ccgtcctctt cgatgatgat
    28081 ggcgctccgc gggaagacgc tgatcccctc tttccagttg tggatcatcg tggacgtgac
    28141 cttggcccct ttgccgacgt agatctcggt cactccgata tgcgctccgt gctctttctg
    28201 ccaggagctc gcgcagccgg agatgatgtt gatctccgag ccctctctcg cgatgaagag
    28261 gttgtggaca tgctgcaccg gctcttcgcg gaggaagagg caggcctgga ggggcatggc
    28321 gaccttcaca cccggctgcg cgatgacgac gaggccctgc gggctctcct gttttgccag
    28381 gtactgcgta tatttgtcct tatcgcgtga tacggcgttc cagtagtaat ccttcaccca
    28441 gtcgtacttc tcaagagcgg tcttgatggg gagcatctct atcccctcag ccccgcaggt
    28501 ggtctggatc acgttctggt cgacctggaa gaaactcccg caccgattct gcatcccgac
    28561 ttcaaggccg gtcagggcga ggcgttcccg gtcctcctgc gagagctgct caatatcagt
    28621 tacatctgtt tgcaacgcac acactccccg taccctttct ctttgaccac gcgcaggatc
    28681 tcgcgggggt tcccgtgaca ccggatctgg ccgtcgatca gtacgtgccc ctggtcggcc
    28741 tcgaggtaat cgaggatgta gccggtgtgg gtgatgatca ggccgctccg atgacggttg
    28801 atgatatgca catctttttc gagcaggcct gcaatctcct ttcccatcag gctcatattc
    28861 tcgaggtcga cgccgctctc cggttcgtcc agcatgagaa aatcgggctg ctgaaccttc
    28921 agttgcagga cttcgctgcg ctttatctca ccgccggaga acccgacgtt ggtatcgcgg
    28981 gcaagaaagt gttccatgtt gaccgtctgc gcgagcgcct cgatcgcgtc tccccccata
    29041 tgggatgtgg cggcgagcag ttttccgagt ttgagcccgg atatcgtagg cgggtgctga
    29101 aacatcatgc ctatcccgag gtgggcccgc tcgtggatcg gcatcccggt gatatcctgt
    29161 cctttaaaaa cgatggatcc ggacgtgatc gtatagccgc taaaccccat gatggcttta
    29221 agcaacgtgc tctttcccga gccgttcggc cccatcagga tatgggtctc cccctgcccg
    29281 atatggaggt tgatatcgtg gagcttctcg gcgccttcca cgttcacgtg taaatcactg
    29341 atatccagca tacggcactc cgttccatgt atttgtcagg gaatacatta acacttgcca
    29401 acggaacaat aattgtttcg acggataaga atccggagcg tagcgatatc cggcatttcg
    29461 ccaaaatggg cgcaaattcc tcgaaccgct acgtaatggg tgcagacttc tgagcgggct
    29521 cccggctctt cactttcacc ccgcacgcgg cctacggtta aatagcgacc tcgccccgtt
    29581 ccattatgga aagatccgca ttcaccacgt ttgcctgcct tgccgagcgg gccgcccggt
    29641 atccgaccgt tgttcggcgg agccagatgc gtaaaacgat gtatctcgcc gccaccgtcc
    29701 cggcgaaccg cacccttcgt gaggcagaga gcctggcgta agtccccccg gtcaccccgc
    29761 gaggtactcc cgggcccgga ggtatgaccc gggccggtcg tgggcgtggg tgatcaggta
    29821 attgtgaagg tccacgatag ccctcgactc ctcgagcagg gcgttgtagg cttctaccgc
    29881 gtcgttgtag tcccgggcct tccggttgta ttccgggacg agacggttgt actcccggat
    29941 ctccccggtc cgggagaggg ctgtcagccg ggcgccgagg gactcgaggg cctctctctc
    30001 cgcctcgatt tcccgggcgc gtaccgcgag ttccggcccg agcgcctcgg cccgaccgcg
    30061 ggatagggag agggcacgtt cgatcgtatg aatctggtcg cacgccccgt agatccgggg
    30121 cccgtccccg accgggatca ccaggggatc ggcggagaga accgtcccgt ccgcgagcgc
    30181 cgcaggtggg atacccacgt aacttctatt cgtggtctcg atgtaggcga aaggagtgtt
    30241 gcggtaagag cacccggcgc ttctcacccc gacggccatg tgtgactcgt tcccgaagta
    30301 gaggagcgcg accccgtagc cttcccgtgc caggagcccg gcgaggagga ggcttttgtc
    30361 gtcgcagtcg ccctcagtgt ccacgtaggt ctcgatcggg aacttcggct ccacgatcga
    30421 ggcgtcggtc tcatacggga tggactgcac gaacgcggcg atcagttcga gatatcggtc
    30481 gtcgtcaagc ccctggcggt cccggatctc acggagggcc gcgagcaggt ccgcgtaaaa
    30541 gggctcctgg tgggagtcgt tgacgaatgc ccgataatag atcgggatcc actcctcttt
    30601 tgagaggtct tctgagaggt agagttgccg atcggcctct ttcgcaccgg cgtagaccgc
    30661 cgggtcgggg tcgatccgga tcgtctcttc ccggtccccg aaggggaagg tgtaggtcgg
    30721 caggtcgtcg agggccgccc cttcgaccgg catgatcgct ggcggaacga ccggggggtg
    30781 gaggaggccg cccaggcacc cggccccgag ggccgcggcg acgaggacga gcacgatgag
    30841 gattcgtttc cacatggtat cagagatagt ccgggaggtc tgccagcgag tcgatgatga
    30901 ggtccggtac gatgcctgag cggcggaccg tctcttcccg gtacttgccg gtcctgacca
    30961 ggattccttt cataccgcag gcccgggcgc cgccgatatc ggtgacgatg tcgtcgccga
    31021 ccatcgccgc cttatccggg tttatcccga tctcccggag cgcacggagg aagaacgtcc
    31081 ccgagggctt tccgatcacc tccgcctcct ttccggcggc atactcgagc gcagccacga
    31141 acgggccggc cgagagcatc aacccgtccg accccatcca gtagcggtct ttctcgagcg
    31201 cgatgaggtc tgcgccgtcc atcaggaggc ggaacgcccg gttcatcgag gcgtaggtga
    31261 gaccctcggc cgcgtcggcg accacgaccg catcggcccc ctcctcgaca gccgcgatgc
    31321 cggcctcctc aaaatccgtc cgggcatcgg gggtggtgag gaggaaacac cgggtccggc
    31381 ctccttcccg caggtgtgcg gcggcggcga ccggggcggt gacgatccgg gtctccggga
    31441 tgacatagcc gagttcctca agacggcggg caaccgaacg gcgggagcgg cgggtggtgt
    31501 tggagacgaa ccggtagggt atcccccgcc ggtcgagttc ccggagcgcc cgatccgctc
    31561 cagcgacagg ccggtcgccg acgtagagga cgccgtcgat gtcgatcagg actgctccga
    31621 tcttcatatc tctccttctg ttgtccgcag gagtatttat gcggtgcccg ggaaggcgcg
    31681 acgcaatcat gagaaccttc atgtggactg caggtgtacc cgcctccgat ccggaaacca
    31741 tcacgaggag aggaggacag gatatggatg catttgaacg aataccaatg gacgcacgat
    31801 ggaaactcgc cgcccgggag gtctccgcgc tgccgcttgc ctaccggaga gccatcaggg
    31861 acggcgccga cgaccagctc tacaacgaga tcgagcgggc ggtgtggcag gagcagggca
    31921 gagaagtggg cgtcatcgcc cgtgccttcg ggatgccgct tggagacgcg cgggaggttg
    31981 cggaggcgtt tgcggcggcg acaggggtgc tcttcgggcc cgagtaccag tatgagatca
    32041 gggattccgc gccgggcgag gcggtcctga ccgtccggaa gtgtgcgatg gtcgtgcggg
    32101 cgaaagagca gggggagaac ccgcttgtcg tctgcaacgc ctgccatgcc tacgcaaacg
    32161 ccgcgatacc ggacctgaac cggggctaca ccccaacgta catgagcgct atctgtgccg
    32221 gcgacggtac gtgctcgatc gcagtaaagc ccgcacggcg ggagtgatgc gggggagacc
    32281 gatctttcgt tcactttcac cccgcggcgg ttatcttgaa tgccggcagg atccaacctt
    32341 ctgtcgacag gatggtttgc ccgttcgatc aggcagggag gctgtggcgg ggccgggttg
    32401 accgggccgg ggagtacgat ctcgtcggcg ggagcggagt ctccgccccg ggcccctggt
    32461 cctcgttcat ctgccgctcc gactacgata gggctcgaag gctccgcgac gacctcgtcg
    32521 ccgggtaccg gggccgggca tttgaggatg tcttttgcgg tcgggaggtt gcgtgccctt
    32581 ccggggcgtg ttacgtcctc gaatcccggg atgccatgga ccttgacggc agggaccctg
    32641 accgggcggc ggcggctatc cttgccgacc tcaccctggt ccacggcatc ggcgaggtga
    32701 cagagcgaag gttgaagggg cggggttacc ggaccctcgc cgatcttgcc cggcaccctc
    32761 tctaccgccg ggaggccgca cggctgcttg attcggtagc gggagggaac actcgcgatc
    32821 tcgtggaaga gatagggcgg cggcatcgac ggagcgaccc gctcctcctc gaagccagcc
    32881 ggttccatgc ccccgaggat tttgtcttcc tcgatatcga gacgctgggg ctattttccc
    32941 ggccgatcat cctcatcggc ctcgcccggg tcggggacgg gtcgatcacc acccgccagt
    33001 acctgctccg gtcggtcgtt gaggaggagg cggcgctcac cgctgtcctc cctgacctgg
    33061 aagcggatgg tgcggccctc gtcaccttca acggccgggc gttcgacctc ccctacatcc
    33121 gggagcggct cgcctactac ggtatcccgg cagacctcga tatgccgcat ttcgacgtcc
    33181 tccactttgc acgccggcgt tggaaggact cgtttgccac ctgccggctc agcgcactcg
    33241 agacggaggt ccttggcgtc ggccgcgaga acgatgttcc gggccggatg gtgccggagt
    33301 tctacgatgc ctaccagcgg accggaaacc ccggcccgct cgtcccggtg gtggagcata
    33361 accggcagga cctcatctcg ctcgcccgcc tcttcgcgct tctcaggggg gatggcgacg
    33421 gcggtgcgtg acttcctcgg cctgctcgcg accgacgaca gatttcggga gcgtgtcgtg
    33481 catatcgaga ccacacctcc ccgggaggcc cggtacggcg gcctctcccg gcccctcccg
    33541 gaaggactcc gggcctactg cgacgggagg gggatccggc tctacacgca ccagtgcgcg
    33601 gctatcgagc acctgcgggc gggggagaac gtcatcatca ccaccccgac cgcgagcggg
    33661 aagacgctcg cgttcctgct tccggtcttc gaacgcctcg ccgccgaccc cgccgcgacg
    33721 gcgctcttcc tctaccccac aaaagcgctc gccaacgacc aggtgaaggt ggtccgggag
    33781 ctggagaaga tctccgggat ccgggtggac gccgccatct acgacggcga cacccccggc
    33841 gggaggcggc cggcgatccg ggagcgttca cgggtcgtcc tctcaaaccc gcacgagctc
    33901 caccagatcc tcccctggca cgcgaagtgg agccggtttc ttaagaacct ccggttcgtg
    33961 gtggtcgacg aggcgcatcg ttaccgcggc gtttcggggt cccatatggc catgctcctc
    34021 cgccgccttg cccgcatcgc cgggcactac ggtgcccgcc cgcagttcgt cctctcctcc
    34081 gccacccttg caaaccccgg cgagttcgcc gcccgcctga ccggggaggc ggcggtctct
    34141 atcgaggaag atgggtcgcc tcagggagag aaagcgttcg tcctctacaa cccgtttgcc
    34201 ggaggcagcg tgcagtccgt ccactccgcg acaaaagacc tcctccttgc gtcggtggag
    34261 agcgggtgcc ggacactctg tttcaccgtc tcccgcaaac tcgccgaact gattgcggtc
    34321 cggtcgcggg aagtctccgg gataagcgcc taccgggcgg gctatctccc gaaggatcgg
    34381 cgggcgatcg aggacggcct caaagacggt agccttcgcg gtgtcgtgac gacgaacgcg
    34441 ctcgaactcg ggatcgatat cgggtcgctt gacggcgtca tcatctccgg gtaccccgga
    34501 acgatgatct ctgtccggca gcaggcgggg aggtccgggc gccgttccca cgactcgttt
    34561 gccgttctcg tagcgtttga gaacccgctc gaccagtact tcatgcgcca tccgcgggtc
    34621 ttcttctccc ggccctgcga gcacgccgtc gtggatacga caaaccccta catcctctcg
    34681 ggccatctcc tctgtgcggc ggcggaactc cccgtcgacc cggagcgtga cagccactgg
    34741 ttcggtccgg atctcgtggg gatgcttgag gcgttcgagc gggtccatct gctccggaag
    34801 gcctcacggg gctggatcta cgccgggcag gggcgggcgg tggatacggt cgggctcgac
    34861 gccgtcactg ccgatacctt ccgggtgatg catgggggca ggctgctcga gacgatggac
    34921 cactggcagg cctaccggga ggcgcacccg ggtgccatcc tcctccaccg gggagagacc
    34981 tacgtggtga ccgggatgga cctcgagcag cacaccatcc gggtggagga gaccgatacc
    35041 gatacctaca cccggcctct ctccaccacc gacgtggcgg tcgtgcagga actccagcgc
    35101 cgcgatttcg gcggcttgca ggtctctttc ggcgatatca aggtcaccga ggaggtcgtc
    35161 gggtatacgg tgcggcgcta cgaccagacc gtgagccacc accggctgga cctcccgccg
    35221 ctccgcctca cgacgaaggc cctctggttc accataccgg accggacggc ggaagcggtc
    35281 gctgccggag ggtgggacct tgccggcggg ctgcatggtg cggagcacgc cttgattgcc
    35341 gtcatgccct accatgtcgt ctgtgaccgc cgcgatatcg gcgggctctc gacctctctc
    35401 caccccgcaa ccggtgcacc gacgatattc gtctacgacg gctacgaggg ggggatcggg
    35461 cttgcggaga aggcgttcac cctccttcct gcgatcgccg ggatggcgca cgaacttgtc
    35521 cgcgactgtg cgtgcaccac ggggtgcccg gcatgcatct actccccgaa gtgcgggaac
    35581 cagaaccagc cgcttgacaa ggaggcggcc gttatcgtcc ttgacgacct tgtcggccgg
    35641 ctctccacgg acgcccaccc caaaaacccc tgatcgatac cctcaagtcc ccgtctgtcg
    35701 atacccgccg ccggggtccc gccatgagta tcatcctcgt tcctatcggg aaggtcgatc
    35761 cagcagagat gcagatgctc gaggatcttc tgaaggcggc cttctctcgt gatgtggtga
    35821 tcggggagga gatcccgcgt cctgcagccg cactgaacgc cgcccgcaat cagtacgatg
    35881 cagagatggt gcttacgttc ctctcgctct ccggggagac cgccggctgc gaccgggtgc
    35941 tgggggtcac caacgccgat ctctattttc cgggcatgaa ctttgtcttc gggatagcag
    36001 gagggagatg cgccctgatc tccctccacc gtctcaggca gccgttctac agcctccccg
    36061 aggacccggt tgtcttccgg caccgggccg tcgtggaagc ggtgcatgaa ctggggcaca
    36121 cctttgggct tgcccactgc gaagaccccc ggtgcgtcat gcgcttctca aacaccatcg
    36181 ccgagaccga ccggaagggg ccggcgttct gcacgacctg tcgtctgcgg gtcttcgccg
    36241 gggaggtcgg acggcgctga ccgggttcct gccggagccc gggcgggggt gctgtgcccg
    36301 gtcagcccgg ggtcgatagg acagaaagaa ttgacacgta tctctcgaaa aagcggaaaa
    36361 tagttcccgg atgctgcgct cttcccgacc tgaatagatc aaaaatacac c
//
LOCUS       DAJB01000112           10662 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_41502, whole genome shotgun sequence.
ACCESSION   DAJB01000112 DAJB01000000
VERSION     DAJB01000112.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10662)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_41502"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    4275..4335
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1994 a         3263 c         3222 g         2120 t
ORIGIN      
        1 acgatgaagg ccttcccggt cgctacagcc tcgaaggacg ggtggccgaa cgtggtgccg
       61 atcgggttcg tggaactcgt cgacgacgag acgatctgga ttgcggacaa cttcatgcag
      121 aagacgcttg caaacgtccg ggagaacccg aaggtctcca tctacgtctg gggacccgag
      181 acgaagggct gcttccaggt caagggcgac gtcgaggtca ggtccgaagg cccggagttt
      241 gagaagatgc agctcaccgt ccggtcgaag atggcgaagg cgccggcaaa gaacctcctc
      301 gtcatgaaga tccgggaggt ctacgagtgc tctcccgggc cgaaggcggg agagaagatc
      361 ctttaaaaac atttttgatg gcctctgccg gggttgtttc cccggccccg tgcgggagag
      421 ggggtgagca tcgggagggt gtgatcctct cactgcttct tccctctggc cggtcgcgac
      481 tcgtacaacg gtaatcacat tctttttccc ttcgggaagc cgcgacgcga agcccttcaa
      541 tctgcccgga ctccgttccc ctcatatctt tgatgttctg cagggacttc ttagagtatc
      601 cgcccataat gccggtcgtt aatgggaggc gtagttctca atggacgggg atggggaaaa
      661 gaaaaagatc aaaatgctgt cattgtcgga gaaattctct ggttgatcga gtttgcggta
      721 gtgccctacg aaacaactgt taatagcgtt gttgagatcg catccgggtg ttcccgattg
      781 attgcaaccc gggggcacgg ctttccaccg ggtatcgcca tgactgcccc cgcccctggg
      841 cgggggagga ggcccctccc cgtcagcccc acnntggcga tagccaccac ggtccacggc
      901 atgagggcat gcgtgagaaa aaaggccgaa ctcaggaaca ggccaggtcc ggcaccaatc
      961 tcatcgggga agaatgacag ccggaatagg gcttcaccag agcccgataa gggaaatctt
     1021 ctccccatgt tatttgttgt catgaacgcg gggtggtgcg caacgtaatt cggccggatt
     1081 ctctgagatc aattatcagg tatatgctgg agcactaccg agtttgcggt gacctcttcc
     1141 gccatcgccc tccattttag aacatggctc agcccactgc cgtggggtga acacgtacgt
     1201 gtccgtgtcg gcggatgcaa cagatgcccg ggaaatattc aggcatataa atagaaataa
     1261 atattaccgt attccttctt cggccggcaa gtacgaagca cccctccgtc aagccgacga
     1321 ccgccaccgt cctggtggcc tcatccgatc acacacccgg gtgcatctgc tgccagagca
     1381 cacaggagat attggaatgc tgaacaaaac atgcattggc atggccattc tcgtcgtcct
     1441 ctgcatcggt ctggtcggct gtgcaagcgc acaggagggc atcccgacgc cggacggatt
     1501 cgagaagacg ctggtctaca tgggtgcggg caagtacgac ccgtcggtcc agccggagga
     1561 ggggaacctc accgagtggt accacatgga gatcatgaac cggacggacg ccgagatcgc
     1621 tgaagagcag caggcggccg acgcctactt cgaagaacag tttggtcccg gcctgccgcc
     1681 ggcaatggcc ttcggctccg acccgcgcaa cgagtaccgc gcctattacg tctccaacga
     1741 atcggcgccc ccggacggct gggtcgtccg tgacggcggg ttcatggtca tgatccccga
     1801 aggcggcatg aacctgaccg gcacctgggg cggcgagaac ggcacgttcg ttccgggcgg
     1861 atccttcctg gtcgtcggcg actataacat cctcatgagc gaagggaacg caaccgacaa
     1921 cgtgaccggg aacgcgaccg acaacgtgac cgggaatgct accgacaaca tgaccgggaa
     1981 tgcaaccgac aacatgaccg ggaatgctac cgacaacatg accgggaatg caaccgctga
     2041 agagacgatg atcatccact accagtccgc cgaaccgatc atcccgagcc aggaactgga
     2101 cggcgctttc ttcttccggt gcactctttc cagtgacgaa ttcggcgagg ggatcgccca
     2161 gggcgtgagc gctccgcaga tggagggcaa cgtcacccag gcgaacatcc gcaacgtcct
     2221 gaccttcccg gcttttggtc catcgatcga ccatactgcg ggaacggcct gaagcgggcg
     2281 tttcggccgg ctgagcggcc gatcccccct cttttttaga accggcgcgg atcggtctct
     2341 acatcgacga cgaccggcac gttcatccgg accgcctccc cgatcgccgg gccgagcgct
     2401 tcgggcgtat ccacccggat gcccgccgcc ccgcaggcct ccgcatactc gacgaaatcg
     2461 gggttgagca gatccgtccc gaagtttggg tactcctcca tcatctgctc gacctggatc
     2521 atcgcgagct gccggttatt cagcacgacg accaccatcg gcagatcgta cttcaccgcc
     2581 gtgacgagat cagccatcgc catggagaac ccgccgtcgc cggtgatgca gaccgccgtc
     2641 ctctccgggt aggcgagttt cgccgcgatc gcagccggga acccgaaccc catcgtcgcg
     2701 aggtagcccg acatgaccat ccgctgccgc ttcatccgga agttccggcc gaaccaccag
     2761 cagttctccc cgacgtcgac ggagatgacc gcgtcgtccg gcagggtctc cgagagcacc
     2821 ttcatgatgt acggcggacg gagcggcacc gccgcagcat ccgcctcccc gtcgcgctga
     2881 cggtcccatt cctctttaag cgccccgatc tgccggagcg ccgtcgggtc gtcccgtccc
     2941 cggatgcgtt ccagcagttt cggaagcacg taccggcagt tcccgtacag catcactccc
     3001 tcaggaccct tcccgagttt ctccggatcg acgtctacct ggacgagggg cacgtcctcc
     3061 gggacaccgg tcagtttcga gaacccgaca ccgagggtga tgagcaggtc cgccccgtcc
     3121 gcgagcttcc tggccgacgg tgtaccgaca tcgccgagga tgctcaaaaa ccacggttcg
     3181 gtctccggaa cgatgccttt tgcccggaac gtcgtcaaaa tcggcgcttt aatcttttcg
     3241 gcgagagcga cgacactccc ggccgcgtcg taggcgcccc acccggcaag gatgaccggc
     3301 cgctccgccg cgtcgatccg atcggctgcc cgccggagat cggcatcgac cgggacgatg
     3361 tcgagggacg gcaggcagga ctcgcgggtg caaaaccccg atgcgagcgt ttctttctgg
     3421 atgttgttcg gaaccgagag ctgggcgacg ccccgccgga gaaacgcgta cttcagtgca
     3481 gtcgtgacga ggtgcacggt ccgggtttta tcatatatgg tgttgttgaa gaccgcgatc
     3541 ggccggaaga aggcgtcctg gtcgatctcc tggaacccac ccggccccgt gtactggaac
     3601 tccacctggc cgttgatcgc aatgaccgac gcccgatctt cttttgcatc gtagagcccg
     3661 gtcgcaaggt tcgtcgcacc cggacctgca atggtgacgc aggcggcgat tcccccggtc
     3721 agcttgttgt aggccgaggc ggccatcgcg gcattctctt cgtggcggac gacgatatac
     3781 cgaactgccg ggttcttccg gatcgcatcg acgatcccga gcgacgacgt ccccgggatg
     3841 ccgaaagcca ccgtcactcc ccatcgtgcg agctcttcga cgatgacgtc agagacggtc
     3901 gtctctcctt cgccttcggg cttcttctcc ggcgcgggta caaacgcgtc cttccctgcc
     3961 cggcagaccg ggcaccgcca atcgtcgggg agcgctgcaa acgccgttcc tgcaggtgcc
     4021 ccaccttcgg ggtccccgat cgcttcgtca tagatgtatc cgcagaccgt acatcgccat
     4081 cgcgccatgc tcaccctcct ctttctattc gggatactgt gctacacaca gctccgcctt
     4141 atatcaggtt ttccccgcac ggcgcaacgc cggctgctat acgtgcggga tatccgtatg
     4201 gttcgagatc tcagagttcg tcgtgcagat atgccgtatc ccgagggcgt ggacgtcgct
     4261 gttcccagtc aggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnncgctc ccgatggcaa cggcatcggc accgagtgcc agtgctttgg
     4381 caaaatcgga ggagatacgc aacccgccgg tcaccacgag cgagacgtcc ttcgctcccc
     4441 gccggtcgag atgccgccgg gcacggaaga gggcatagat cgtcgggacc gaggtcgccg
     4501 ccttgacgta cttcggagcg gcggcggtcg cacccgggcg gccgtcgagg gtgatgaaat
     4561 cgggcccggc atacagcagg gcgtcgaggt cgtcctcgat atctcccgcc gcgatcttga
     4621 tcccgatggg tcttccgccg gagacttcgc ggagccaatc gacctttgcc ttcagatcgt
     4681 cccggttcct gatatcctcg aacgtcgcgg ggctgacgat gtcggtgcct tccgggtagc
     4741 cccggacccg ggcgatctcg ggggtgacct tctcggccgg gagacgggcg cccataccgg
     4801 gctctgcgga ctgccccagc ttgatctcga cggcatcggc ggctcgcagg ttctcttcgg
     4861 tgatgctgta gcggttcggc acgtattcga ggatgtaacg gtaggcgtgc tctcttgcct
     4921 cgggcagcat cccgccctcg cccgacccca tcgccgtccc gacggcggcg cttccttttg
     4981 cgagcgcgat cttcacctcc cgggaaaggg cgccgaacga catatgcgtc acgtagatcg
     5041 gggtctcgat cacgagcggt tgcttcgccg caggcccgat gatcgtccgg gtggtgacgg
     5101 gaacgtcctc gttgagggga agcctggcga tctgggcgcc tttgatcagg atgtcgtccc
     5161 aggagaccac cgggagtttc gtgcgcatcg gctcgatgag agattccccg gacgcggata
     5221 ttgcatggat atccgccatc cagacctcga ggtcgtctga ccgcctcgtc cattcgccga
     5281 ggtagccttc gaggtctttg tggtcgaggt gagtgaccga gacctcggtc ctcgccccgc
     5341 agaccgggca ggtgaagacc ttcgtctctc cgggcgcttt ctcctcgtcc cggatcggct
     5401 tgaggtgggt cttatcggaa ctgcatatcg ggcacttcca gtcttcggga aaatcttccg
     5461 gctccgtgcc gggtttgatc cgggtgaggg agtttccacg ccgggtgtca tattcaaagg
     5521 tattgcagac attgcaccgg tagcgggtca tcatctccct ctcctgagat tactgtccca
     5581 actgagtttg ttccgaccct tttgtgcagt agtagatctg cttgagcccc atcttttcgt
     5641 gtaccgggca tcctccgcag atacatccct tctcttcgga tatgcaggcg ctcttcccga
     5701 tcgtgacgaa gcaaaatcca cccccctcgc cgcattccac ccaggtgggg cattttttac
     5761 aggtgcacat cgaaaggacc atcttcttct cttcaagttg ctgctggttc gtctccattt
     5821 tcttctcctc tgtaccgtgt ttggacacgg tgtattcttc cgatatctaa tgaacgtttt
     5881 ggcgaaacgg ggctctgcga gggcgtttag tgcgcaccgg ggcgatcgtg gtgcaccgga
     5941 tcccccacgc tgcggttctg gagccgagcc ggtgatttta aataatcatg cctcactatg
     6001 gaccgcgagg aacgcctatg gacaagttcg agatgctgat gcagaatatg cgggagatgc
     6061 cgcccgaaga gcagatgaag atgatggaga gcaaccgagc ggcatgcacc tgccccggct
     6121 gcccgacgta cacctcatgt gcgaagaatg cagatgaacg cctcttctgc aataccggga
     6181 agagtttcat gtgcatctcc gaagagaaga gctgtctatg ccccacctgc ccggttgcgg
     6241 agaagatggg cctgaaatac cagttctact gcacccgggg aagcgagaaa gcccaacgct
     6301 acgagagtac gctctggggt acaaagatca tctaaccctt ttacaccgcc cggcgatatc
     6361 gtcgcggtac gggccgataa agcggcgccg gttgacgagg acgcagacgc ttgtttgccc
     6421 ggagagtgcc gatacccctc tgcgccttga gttcggcgat gaattcctcg atgagatcga
     6481 tgtcgctctt atcgagcctt ccttgagtcc acgggcgatt gagttgtacg cataggctcc
     6541 tttggcatga tggaattctc ggtccttaac aggcagtgcg gctaagctct gcatgaaaaa
     6601 gagatcaaaa gaaaggtgcc acgcttttcg ccgcggttcg aatgtgaaca ttgagcgccg
     6661 ggagggagat ttgaactccc gaggtgccag gcaccagtgg ctttcaaggc caccgccttc
     6721 ccggactaga ctatcccggc tcgccgttat ctattagtcg gcggataaaa aaaggttgtt
     6781 catccccgac gtgcgaggac gaccacgaga agtgcgagga gtgccagcat caccgggacc
     6841 ggcgcagcct gggcgggggt gggggtcgcg tccccgaaga gaccgggcgc ggtctggctc
     6901 accctgaccg agtcggcatt cgaggccgag acctgcaccg ttcctctgtc ggtgtaactc
     6961 ttagggtaga ccttgacgta gaccggacct ccccgtgttg caacatctac ggtctcggga
     7021 tcgagcatgc ccttctcgtc cacttcatgg agtatgttat cctcgtcgat gtaatcgacc
     7081 acccagttga tcccggccga ggttgcgatc gtcaccgtgc cgctccgggt gtcgacctcg
     7141 aagtacgccg gggcagatcg tgtggcgggg gcactggccg atatcatcgc ctgcgtcggc
     7201 gtcggacccg gtgtaccggt caccacaact acgacgctgc cgatgtagcc tttggcgtcg
     7261 gagaaggtga cctcgtacga gcccccggca gagatgggca gttccgtaga gaacgccccg
     7321 tccttgccgg tcgcgacgta cttcggcccg tagacgacgg caccgtcgtg ctcgacctcg
     7381 atctggacgc ccgcgtcccc gatatcaccg atcgtgcccc ggagatcgag ggtgccgtcg
     7441 aagtcctggt tgagcggaga ggttatcgtg agactcccgg accggtcgac cagggtgacc
     7501 atccgctgtt tcgtcgaact gctcccgaag gtggagagcg tggggtcgat gagctcgatc
     7561 gagtatgtcc cggcttcaag accggtggtc tcgaattccg cggagaacga accgtcctgt
     7621 tgcacgatga tcgtcttcct gcctacttcg ctcttccctg gcttgagaac gtataagatg
     7681 atctccgtgc tgaaccctgg atcgagcgaa ggcctgggga gcccccctac aatcgtggtg
     7741 ccggtgactt cgagcggttc gccgacgtat accgtctgtg gagcactgat tgcgacgtac
     7801 gcggctgatg ccgtactgac gagcatcatc gcaacgatga gcgtcagcca tgcgatcctt
     7861 ttcataccag tacatcaacg aatatgaggt ataatgctat ccaaattgac tgagaagccg
     7921 ctggcgtcct ggcgcgggaa agacctctat ggggggcaga tcctcgatac gctgacggtt
     7981 atctttcgga gcggcgggtg ttcctggaac cggtgccgta tgtgcggcta ccggcacgag
     8041 cgctacccgg acctcccccg ggacgaactt gccgaacgga tgatccgcca ggtccgctgg
     8101 gtgaaagaga atttctccga cgaggactac caggtgctca agatcttcac gtcggggagt
     8161 ttcttcgatc ccgacgaggt gccccccgcc gtccggcgcg ccgtcgcgga ggcgttccgg
     8221 ggcaaggtcg tgatcgccga gacacggccc gagtatgtcg agatcgatgc gctccgggag
     8281 ttccgggagg gcatcgatac cggcgcctgg acacgaccgc tctctgttgc cgtgggcctt
     8341 gagaccacga acgacctcat cagggagagg agcatcgaca aaggattctc ctacgccgat
     8401 tttctccgtg ccgcaacggt tgcgcacgcc gccggcgcgg gcatgaaagc ctacctgctg
     8461 atgaaaccac cgttcctgac cgagcgcgag gcgcgcgacg acatgatacg ctccatcaag
     8521 gacattgcct ccgttgcgga cagcatctcc atgaacctct gcaccgtcca gagcaggacc
     8581 gaggtcgaac ggctctggaa acagcaggca taccgaccgc cgtacctctg gagcgtcctt
     8641 gacgtgctga tcgcttcacc ggtgcatatc ctctgcgacc ccgtcggcgg cgggcagatg
     8701 cgggggccgc acaactgcgg tgcctgcgac ggcccgatcg tgaaagggat cggcgactac
     8761 tcgctgaccg gcgatgtcgg gctcctgcgt gccttaagag agacggagtg cgggtgcaaa
     8821 gaagagtggg agttcgtgct ggatcaggaa gagccgttct gtatgccgct cacccgctag
     8881 cacttctcct gttccttcag ttgcttctca agcagcggaa ggaacgttcc cgcatcgctt
     8941 acgacaccga tcgcctgcgt cgtcccccga tccatcaatt ttgtcaccga tgcggggttg
     9001 atatcgacgc agacggtctt aacgtatgaa gggaggcagt tcccgacagc gatggagtgg
     9061 agaagcgtcc cgaccatcac caccatcccg acgtcgtgga tgtggcggcg catggcgtcc
     9121 tgcgcctcca cgacgtccgt gatcgtgtcg gggagcggtc cgtcgtcccg gatggagccg
     9181 gcgagcacga acgggatgcc cttcttcacg cactcgtaca tgatcccccc ggtgacgatc
     9241 ccctggtcga ccgcgttctt gatagacccg gcacgcatga tctcgctgat ggcgcagatg
     9301 tggtgcttgt ggccgccggt gatgagcgta cccgttttga cgtccatccc gagcgacgtg
     9361 ccgaagaggt ttgactcgat gtcgtgagtg gcgagagcgt tccctgcaaa gaggacgtcg
     9421 atgtagcctt cacggatcat cttcgcaagg gcgtcggctg ctcccgtatg gacgatggca
     9481 gggccgccga caagcgcgat cttctccccc ttctgcttca tctccaggat ctcgtgggcg
     9541 atcttcgcga tgatcgcctc gctcggccgc tccgacgaga cggtcccgtg catgaactca
     9601 aacgtactga ccttcctcgg ccgctcgggg tagacgaccc ggaccccggc ctcgctgacg
     9661 acgacggcat cgccggcctt gatcttcgat atcggggtgc agagtgcgcg cttctccttc
     9721 tcgtcgacta caatatggca gtccatctcg atacgctcga caggcagcca ttcgtcctgg
     9781 tattttacga aggtcgggtg gttggtcgtc gagtagaacc ccttcggaag gatccggtcg
     9841 ccctctgccg gtacgagggt gacgtcgctg atctcggggg tgcgtgcgcc gagccgatgc
     9901 agctcggaga ggatggcgcg aagttgccag tcggtgggtg ctgccacgcg cagccgcgcg
     9961 taactggtgt ccgtcttctg cttcccgaca ttgaacgtga ggatctcgaa ttctcccccc
    10021 atatccatga tcctatcgaa cacgagagtc ataatgcctg agtcgatgat atgaccctct
    10081 agttcgattt cccggcagga ttccatatat agggattggt gatggtccga taatacagtt
    10141 tccccgcgtg ccggctgctg gtacccgctc tccagagcgt cacccccgct tgcccggatc
    10201 ggtgatggcg agcagccccg ggcgggcatc gccgcgcacc ggccccgagg gtgggaaaac
    10261 ttcggcgctc aaatttcggc cttattgaga cgctctcagg agagtcatcg attgcgtccc
    10321 agacacggat tccaggggga tatcgccatg actgcccccg cccggggggg cggggtgcga
    10381 tgggccatag ggccggagca tgagcaacga aggtgcgttt gaggaggccg gaggccgggt
    10441 ggggtggggg ttgtagacgt cagttgacga gtagagacag gggagagggg agaggggagc
    10501 atcccccctc cccgtcagcc cctccccgcg tggcgatagt cccacggtcc actgcaccgt
    10561 gatattttcc cgttttgatt actctgtttg cctcatagcg aaggccttcg accttcacat
    10621 gcgacgggca aggaggcccc cggtttcaac cgggggagga at
//
LOCUS       DAJB01000113            2677 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_69541, whole genome shotgun sequence.
ACCESSION   DAJB01000113 DAJB01000000
VERSION     DAJB01000113.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_69541"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          452 a          858 c          816 g          551 t
ORIGIN      
        1 ccgggtatcg ccatgactgc ccccgcccct gaggggcggg ggaggagcgc gaagcgcggg
       61 cggggtgggg ttgatgatgt gtgcgaggtt ttgcaagttg gagacagggg aggggggagc
      121 atcccccctc cccgtcagcc ccacccccaa tggcgatagc caccacggtc cactgcaagg
      181 ggacatgtcg cggaaaaggg caaccccggg gacaagcttg gcccagccct cacctcaccg
      241 ggcgggaaga aaaatcaaaa cgtatccgga gccttcggta tcgcccctct tgaaagttcc
      301 gcctgtgtgc tcccttccgt gctcccggca acctgcgcgt aaaaggtctc aatatcgtgg
      361 acgattgtct gccagttgta cctggtccgg acaagctcct tcgccttctc gccgaacccg
      421 gcccgtaagg cgtcatcggc aagcaggcgt ccgatggagt cggcgagcgc ctccggcgtg
      481 catctggtat tgaacccgac ggtgttgtcg atccatccga gcaggtctcc cttccccgtc
      541 gtcacgatgg gggttccgca gaggcacgct tcgaggaagg tgacgggaaa gccggtgaac
      601 ctcggcgtca cgaagacgtc tgcgtcggtg tacgcagcca tcttgtcttc cttgctcacg
      661 aacccggtga agaccacccg gtcgtcgagg ccaagcgacc tgacccgctg cctgaactcg
      721 tcgttgtggc ccatatctcc gcccaccagc atgagaacgg cgtcgccctc ctcccgtgcg
      781 accagggcaa acgagcggat cagcagatcg atccccttcg tcgggtcgag ccttcctaag
      841 tagagcacga ccttcgtggc gtcgtcgatg ccccacacgg cacggaacct gccgcggtcc
      901 ggaagatcgg ggtactcggc gaggtcgatg ccgttgggga cgatcgtgat cttcccctcc
      961 gcaacaccca gatctcggta gcgctccgcc tccgtctcgt tgagcgcgat cacccctgcg
     1021 gctccgagga tcactttctt cgaccagaac cggtcgaaga gccgcttcat ccgcgtggaa
     1081 cccgtatcct gcgggagcgc cccgtgcgcc tggagcacgt aggggacgcc gtacttcctc
     1141 gcgtagtgcg aggcgatgac ggtgagcatg gtgcggtggt cgtgaatgtg gaccagatca
     1201 aacttgccga tctcctttcg cgcgaccgcc ggcatacggt agggcatcac cggcaggatc
     1261 gtgccccggg tgtattttcg taaattctca aagtagtaga cgtccatccc gtcgacgccg
     1321 gtcaggtggt ttgtcggcag gtcgtttgga tagatgctcc tgtttgttgt atagaccgtg
     1381 atctcgtgcc cgttctcgtg aagggtatgg gagatgtcgt atgctacccg cgccacccct
     1441 cccgactccc agagcggttt gaagaatgga gtaacctgta atatcttcat gcatgtcccc
     1501 ctgtttgccg ctgccggagc cgcctccgga tcagacccgc atagcagttc agacagaacg
     1561 gtgtcaccag ccagaccccg cactcgacca ggggcgcgag cccgtgctgc tggatccggt
     1621 agcggtaact ctccaggttg tatgtcaggt aatcctcgcc gatctgctcg aaccaccact
     1681 gccggttgac ttcaccgttc tgaacccggt ccgagcactt cccgatccag tgctgcacct
     1741 tctcgcgggt ccgcccttcc gccacctccg gctggaagtc gccgttgatg acgcaggggg
     1801 tgacggcgta gtcccggatg ccgtgccggt cgaagtccac cgagaggatc atggtgctgt
     1861 tgatctcctc cttgaagatc tcggtgtcga actggaagtt cccgagggag taggcgatca
     1921 ggccgttgtt ataccgttcc agcccctgga tggtgtgcga gtggtgcccg agaaccaggg
     1981 tcgctccggc atcgatgagg gcacgggcga gtctgacctg gcccggggat gggtagtagg
     2041 tgttctcgat cccccagtgc agggagacga cgatgtggtc gcaccggtcc ttgagtgccc
     2101 tgatgtcgtt gatgatggcc ttcttcttca gtttggctac cgtaactccc ggcggcgacc
     2161 ggaacctccc gcttgtgtaa ccgaggaagc cgagccggat cccgtttcgc tcgacgacca
     2221 gccccccggg ggggtcgtcg ccccgtgccc ctccgatgta ggcgatacca cgggtttcca
     2281 gaccctcaag ggtcttctcg aacccttccc gcccccgatc gagcgtgtgg ttgtttgcca
     2341 cactgagaat gtcgaaccct gcgtccttga gatactcgcc cgcttccggc ggggttgtat
     2401 tgacccagcg tttcctgctt tcggtgccgc tctcggagag gaccgtctcc aggttcccga
     2461 agagcaggtc tttctgccgg agttcatcct ggatcagggc aaacggtttc ttgtcgttcc
     2521 tgatccagag cagcacgtcg ccgacggccc gcagccgcac cgatgtcatg catctcctcc
     2581 ggcagccctt tcgctgttta tagaaggccc gggcctgtag ccggtcttca tcgtcggcct
     2641 gcaggccccc actctcttca gtcccggtgg tggagca
//
LOCUS       DAJB01000114           16718 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_25751, whole genome shotgun sequence.
ACCESSION   DAJB01000114 DAJB01000000
VERSION     DAJB01000114.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16718)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_25751"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2923 a         5236 c         5290 g         3269 t
ORIGIN      
        1 ctccgatgat tctagctgca agcccccggt tttaaccggg ggtagttgac tgccatcttg
       61 ccgatggact ggccccggta cccctcgagc agcgtccagt agaggaagag aacgaccgaa
      121 gagaggctga aggagaggag cccgggatct atcataaacc tccaggcggg cggcagccgg
      181 tcggagagcg cccagagagg cagcccgatc aggagcacgt cgatgagcca ggcccagaac
      241 cgggtctccc atcgggcgag atagagaatg accatattat tcttatgtcg ttctctgtgg
      301 gtcttaacga ttcccctgag ccgggtcggg tgcggccaat ctcgccgcaa aaagaatgga
      361 ttatccgata taactgagtt cttcccggcc ccgggcttcc tctgcttcct ggatattcgc
      421 tatgatctgc tccatattct cggcacgctg ctgcagggag gtatggtcga cctcgatccc
      481 gatcaggcgg gagaggaccg caaggaggct gtcggcgctc ttcgggtcga cgatatagcc
      541 gctcgtctcg cccatcaggc agatcccctc gatgccccgc gcctcgccga ggccgagcag
      601 gagtcccgac gcccccacga tcatcccgtc gctcccggcg ttctcaaacg acccgcctgc
      661 cgcctctacc tccggccgga gcccctccat attgacggca cagagcaccc tcgggttctc
      721 taccaggtgg ccgacaccgt agccgccaag tgcatacacc cgccggaccc cgaggtcctg
      781 tgctatgtcg aggtaccgct cggccaggat gtagtggcct tcggccgagt tgctctggaa
      841 atcccctatg aggaagagga ctgctttccc gtccttctca cagcggtaga tctcgttgtt
      901 gaccagatgg gtgacgccct gctcgtcgac gatcacctgt ggcggaaagt ggagcgaaga
      961 gatctctcct atcttctcgc cctcgagctc atggatcagg tggtcggcaa cgagtttccc
     1021 gacatgtccg atgccgggca ggccctcgat caggatcggg gcgtcgatgt tgccgtctcg
     1081 caaaaaggtt actgtgacgt gttccatctg cgtgcagctc tcctgtagtc gccgtacctg
     1141 tcctccgggg aaaaacgtgc cgggtgggcc ggccgggatg gctggccgca gaccgggcag
     1201 accagagaga gtgtatacct caggtcatcc ggacagcggc gaatgcgatt gctcataggg
     1261 ttctatccag ccttctgctt ccggataaac ttgccggaac cgcccgcccg ctcaaccacg
     1321 ccgacggcgg cgcttgccgc cttctcgatt gccttctcgg cttccttata atcgggtgcg
     1381 gtcactttta tccggtattt cggggctcca acatagatca ggtcgatctc cacgtcctcg
     1441 accttgggct gcgcactccg gagcgcccgg cggattacgt tgacgccatc gggcctcgtt
     1501 gacgtcagaa cgagatgccc cgtgatggtc acccttgaga cctttacgtt ctcgtgcgcg
     1561 acggtgacga gcgccttcat gaccggctca tcaagtttca gccggtctgc cgcctcgccg
     1621 ccggtggtga cgatatcctc aaaggcaggg tacagctggc tatattcacg gtatatggcc
     1681 tcctcgatga cgcttctgtc cactcccgct gcctcagagg cgaacccgac ccatttggcg
     1741 gccttctgct cgttcttcca ctcgtggatc ttttcgcgcc gctggtgctc gtttacgtct
     1801 tttagtgaga gatcgatgtg gccacgatcg gggtccacat tcaggacctt gcagacgacc
     1861 ttctgtcctt ctctgacgta gtcccggatg tgcttaatcc agccccgtgc tatctcggat
     1921 atcggtataa gccctcttcg ctcgtcgtac tcatccaggg tcacgaacgc cgcaaagtcc
     1981 ttgacgtcca cgactgtgca gacaacgagt tctccttctt cgggccactc tctctcatac
     2041 atggaatttc actcaagtac tgccagtatc tcagccttta tatctgcttt cccgccgtga
     2101 ggttctgcaa ggacacgccc gcagacactg caccccacaa tggtactcgc tctctcaaag
     2161 actatctgtt cattctcaca gtcaggacac tttacccgga ggaatgtgct cctgttctct
     2221 ctgttaaccc ggaccataat aactcactcc gtaagttcga atttacccgc ccggaatccc
     2281 ggccggaggt gcgcctttcc acagaccgtg caccggtatc tgacgttgat cttcttcgtc
     2341 ggcttgtcgc cgccgggcac cttgctgaat ttgcccatgt tgcccaccat gctcctgcgc
     2401 cccttctggc ggtcgatcca gttaaaatgg cgcactttgc ctttctttac cttcacgact
     2461 tcatggacct ggtggcttct gcagaacggg cagtatgtct tgaatttgga tggcattttc
     2521 atgatagtag cctctattcg attgatacga gtacctatat cacttgttaa gcctaatatt
     2581 taaggctatg tcgtgctcgc agagcacgtc tgcattgttc tccggcaggg tgacgatatc
     2641 tccccgttcc aacgcgtagg tcctgccgtc cactcccatg aacgactcca tgttggagcg
     2701 gatatgcacg agggcgtagg gagacgagat gggacgggga gcggtggtcg ccggcacctc
     2761 cggcccgggt tcctccgggg tctcgtcgac ccggagagcg gcggccgcac caccccggcc
     2821 gaaggcatac tcctccccgt ggacgagcgc ttcccggcag acctcgatgg cgtcgacgac
     2881 ctgcccgtac atcacccgct ctgccgggag catcttccgg gcctcttcct tatcgtagta
     2941 ctgcccctcc atctgctgga gggcaagatc gaggatctgc cgggtccgga ccgagaagat
     3001 ttcttggacg gtgtcggtga cgctccctat ctcctcgatg agcaggctcc ctgcccggct
     3061 ctccaggggg tttttcaggc cgtagtagtc cgccttgagt ttctcgacgt acgcccgggc
     3121 atcatcgtag aggccgggct cgatatgcga gagccttccc gagtggtgca tgtcgagcag
     3181 catctgccgc agcctctcaa ggaggtcggt tgccatcacg cacccccggt gatctctgcc
     3241 atgttccggc agcagaggaa gaccgccaac gcctcaggga cgtttgccgg tcctgctttg
     3301 agcgtgtagg tgttcccgag cagggacgtg gtgaagtttg ccttcatatc cacccgcacc
     3361 tcccgatcgc cgaagaccac cgcatccacg agcgggtcga agtccgcgaa gtcccggata
     3421 tcgagcggca ccacccgcat cccgctgccg ccgtgcagcg atcccggcgt ccggatcaac
     3481 cgcttgatgt cggtcgtgac cggttcgtcc gcacgtgctc cggcgtcgcg gagcttctcc
     3541 tcaaagtcgg gtgcgcccac gatcgcccgg atgacccgat tctgcaggag gtcttgtggg
     3601 tccttcgcga gaagggcgtc tatatccgcg gggaactccg cggccgcccg cttgctgacg
     3661 ccttcaagcc ctacgaggta ggccgttgcc tccgccgggt cgagggccgc gagatggagc
     3721 aggtgggacc ggagggtctc ggcgtagcgc tgtcgccacc cggtccgggt gctccccgga
     3781 ctgagcatca cgcccgggtc aagtccgatg ccgcagatgt agtcgacgat ctcgcgacgc
     3841 tcctggcttc cccatccccg cacagcgata tcggtgacgt ggatatggta accccgcccc
     3901 ccggagaagg ctacccggag gtggtttttg gcaaagccga gttcttcggt cagcatcccg
     3961 agcagtttct cggtctcttc ctttacccgc gcaagcatca tcgcgtaggg gccgcggacg
     4021 atctggtcgg catcgaggtc gaagatcagg tcggcgccgg cccagtgctt gtcgttcatc
     4081 gtcggcgccg acggcgtctg gtagtaggcc gtcgagtagt agacatgggc ggggaccaga
     4141 ttcttgacgt atgcggcaag ttccagcgga tccataaacg ccatatgccg ccgcatccgg
     4201 acttcggccg tggcatcaaa gaagacgaaa ccccattcgc gctgctcaag ggaggacgga
     4261 acggtgaggt tctgcctctg gtagtactcg gtgaacctct gcttcacaaa ctcgagcgtc
     4321 gcgggcttca tgtgaacgtc ttgatcatgt atgggcccac tcgttcatat ccctgtttcc
     4381 tgtagtaggg ccggaccccg acaccgctca tcactgcgag gtgtccgtaa ccgtggtcgc
     4441 gggcctcctc ctcggccgcg gagaggagct ggacgccgaa actgcggtgc tgccactcgt
     4501 cggcagatgc cggggtgctg atcgggacca cgctcccgta gacatggagc tcgcgcagga
     4561 gcgccgcgtc ggcgaggtcg tcgcggaagg gccggtgcgg gaaccggagc cgggcgaacc
     4621 cgacgagggc gtcctcggag cccacctgga tgaaccgctc ttccccgccg caggcgcggt
     4681 aggcgtaggt cgagaccact gccgcctccg gtgcaaccgt cctgccgacc tcccggcagc
     4741 ggatgcaccg gcaacggagc ccctgcgaat ggagccgcgc ttctgcaagc tgccggaagt
     4801 tgctgtgccg tgaacccgcg acgatcagct ttgccgggat gtcccgctgg atccgctgga
     4861 ggcggacgta ctccggaagg agtgactttc cataggcgat gaggtcgatc agttcctctt
     4921 cggagtaggg caggtactcc ccctgctccc agagggcctc gatctctgag cccggtgtca
     4981 ccagggtcgg gtagatcttc aggaagtccg gccggaaccg ttcatcggag aagagcccct
     5041 caaacatccg ccggtcatcc tccacgctcg ctccggggag gttgggcatc acatgaaacc
     5101 cgaccttcag ccctgcatcg cgcagaaggc agttcgccgt gaccgagtcc gcgaccgagt
     5161 ggccccggcg gttgtagtcg aggatccggt cgtccagctg ctggacgccg atctccacct
     5221 tggtcacgcc catcgcgagc atccggtcga tatgctcctg tttgcaccag tccggcctcg
     5281 tctcgaaggt ggcggcgaca caccggaccc ttgccgactc gttcgcggca aagatcgcct
     5341 cgagatcggg ctttgccgcc gggaccccgg cccgggggta gtcgttcatt gcctgcacgg
     5401 cggcgccgac gaaccactcc tggtaatcga ccggtcgggc ggtcatcgtc ccgcccatca
     5461 cgatcagttc cgccttgtcg acgtggtgcc cgagcgtctc aaactgcccg aggcgggcct
     5521 gcacctggtt gtaggggtcg aacgcgtgct cccttgcccg gagtgccgcc ggctcctctc
     5581 ccgtgtaact ctggggggac gcaaaggggt gctccggccc gccggggcac gggaggcact
     5641 tcccgtgggg acaggggtgg ggcgaggtca tcaccgcgac gggcgccacc ccggagagcg
     5701 tccgcgtggg tttgacgagg aggagcggcc ggaggcgttc gcgctcctcg ggcgtggcgg
     5761 cggcgaggat cgcggagttt ttgggcatac tgatgcagta cttccggcag acctcgagtt
     5821 tgatcttctg gatgtcggca ggaccgggcc cggcagaaag gatgtgggag atcatctccc
     5881 ggaaagtatc ggtatcgttc atcgtctagt gttcggttgc aatccgttcg gaatgaaaat
     5941 cggcgtggta ttcgatgtag gggttgtggt gtgccctgat atggtggacg atctcgtcgc
     6001 ccagggtcag gatagctttt ttatgggtga ccttattctt atggatgtgg acgggtgaga
     6061 tgtggagagc gttgtactgg tcggtcggga catcctctcc ggtaatgctt tcatagtact
     6121 ttctgacgtg aactaatagc atgtgtaagt ggagcagctc ttccttttgc acgattatca
     6181 cctttcctcg aagaatacta ctgtccgcgg gtagatataa tttgttggtg atctttttat
     6241 atggatgaga tgcgggcagt cggggcgttc atcgggcttg caatcggcga tgcactgggc
     6301 gcgcccctgg agggccttcc gccgggcccg atagccgtaa cggagatgtc agggggcggg
     6361 atccataaca cgctccccgg ccagtacacc gacgataccc tgcaggcgtc ggctctggcg
     6421 cagaccctcg tcgggtgcgg gaagttcgat ccggatgact tcgcccggcg cctggtttta
     6481 gtataccggg ccaatccgga gtttttcggg ccgacgtccc gggccgtcct cgacctgatc
     6541 gaggggggcg tccggccgcc ggctgccgcg aggatggtgc acgaggcgcg gggagggagc
     6601 aggagcaacg ggagcgtgat gcgtggggtc ccggtcggca tcttctaccc ccctgatgcg
     6661 gtccgggagg tgagcctcgc cgcctcggcc gtcacgcatt tcgaccccgt cgccggcgaa
     6721 gcctcggcgt tcgtcaaccg gatggtcagc gggatgtgcc ggggagaagg ggccgctata
     6781 gccttccgcc gggccctcgc cgcctgtcct gatccggaac tccgggggct gctcgaggac
     6841 taccgggcgt acccgctcga gccctcgctc gacgccgtcc tctgcacgca ctgcgccgtc
     6901 agggtcttca tggacgcccc gtcctttcgg gatgcggtgg tcacggcggt caacctcggg
     6961 ggcgacgccg ataccgtcgg cgcgatcgtc ggcggactcg ccggggcgca ttttgggtgc
     7021 cgggccatcc cggcgtcatg gctctcggcg ctgtgcggcc gggaggacct catcgctctt
     7081 gcccggcggc tcactctggc gagcggtccg tagcgtcaga tcgggcggag ttccaccagt
     7141 ttcaggggcc ggccacgctc gcagacgtcg ggggcgttgc cgaggacctc gccgacgatg
     7201 tacttctctc cctcgatgat gccgtcgggg cggcagagcc ggtaactccg gcagcctgtc
     7261 ctcggacagg agaactcgta actgactttc gagttcgcta tcgccatgtc ggcgcctatc
     7321 agcgcgacga tgggggcctc catcacatcg accgcgacca ctgcctcgtg gtggaccggg
     7381 cagtcatgcc gggtgttgga gcggacggta aggatccggt acttcttccc ggactgcagg
     7441 ttatggcaga ccttgcgcac cttgcagccc tcgcactcgg tgcatacgct accctcataa
     7501 acgaaatcaa gccccggctt tgcgagcgcg gccccgaaca gcgtcacctt tgtcttcggt
     7561 tttaccataa tctcattcac tcccggtaga gcatcttcac gaggtcacgc gccgactctg
     7621 ctgtcagccc catatccagt atggtgaacc gttcgggccg gatcgtccgg gcggcgagca
     7681 gcgccgcaac agccgtctcg tcgtcgaccc cgatctcggc gggggtggtc ggtgcaccta
     7741 ccctccgcag tgagtcgcgg atcccctccc agtccccgcc gtggaggtac atcgtgatga
     7801 tggcgccgat gccgcacttc tccccgtgga ggccctttcc cggtgcgagc ctctccagtg
     7861 cgtgggagaa cttgtgttcg ccgccgctcc cgggccggga cgaccctgcg atgctcatcg
     7921 cgacgcctga agataccagc gctttcgtca cgatccaggc actctcctcg gagtgcggtt
     7981 tgatgaggtc ggcgttctta aagaggatct cggcggtcat ccgggagagc gtcagcgcat
     8041 actccgagac tggttcgcct cgaagacgat gggagagttc ccagtcgagg attgcggtgc
     8101 agtttgccat gatgtccgca cacccggacg cgagcagccg gtggggtgcc gatgcgatga
     8161 tggcggtatc ggcgacgacg gcgatgggcg gttcggccgc gagcgagacg ttcccctcgg
     8221 cggtcgggac cgaggccctt gatgaggcga tgccgtcgtg cgacgcggcg gtcggaacgc
     8281 tgatgaagtg acgatccgtg ttgtaggagg cgatcttggc cgtgtcgatg acccggcccc
     8341 cgccgacgcc gatgacgaac ccggcctctg ccgccgccgc ctcggccttc ccgatcgtct
     8401 cgagcgggtc gctgccgtct gcggaaaacg tcgtgacgtt ataagagccg gcaagaagcg
     8461 cctcgacccg gcttccggca atgtcccgtg tccgaccacc cgatacgatg agcacagagt
     8521 cgccgagagc aaggtcttca cagacagccg ggatctgctc gaggacatcg tggccgatga
     8581 cgacatcacg ggggagctgc atccatttgg acttgtcgaa gaccttggtt ctgagtaggt
     8641 ttatgcggtc tgcgctcatt tagatcagta atgattaggg agttcctcta caaatattac
     8701 atagatccca tccgttacgg tgaggcgtat acgctcgtcg atacgctgac ctatgccctg
     8761 atccttatcg cagccgtcta tcttgtctac cgggggctcc ggcggtacag gatcgccatc
     8821 gacgacgaac tggtgcttgc gaccctgccc ttcgtcgtcc tcggcgggct cctccgggtc
     8881 gtcgaggata ccgggatgat cacctccgac ctccattttc tcctgatcac gcccctgatc
     8941 ttctttacga tctttgcggt tgcagcgatc gccctctttc tcggaaaact cacggagaac
     9001 gccggactca ccgcccgcta cagccgggtc tacggggggg ccggcatcgt tgcctgcctt
     9061 atcgccactg cggcgctcgt ctggttcggc ctcactgagg cgacgattgc gctcgatgtc
     9121 ctcgccgtca tcctcgctct cgcgacagtc acgtcgctcg ccctctgggc attccttgtt
     9181 tacgtgctaa agtgggatta cgcctcaaac atcctctata aacttctcat cttcggccac
     9241 atgctggatg cgagcgcgac aggttacggg atcgatatcc atcccgtcca ctacgtggag
     9301 cagcatgtcg tgggctcggg cctgatcgag gcgaccggca ccgcgttctc catgtacctc
     9361 ctcaaactcg cggtgctcat ccctgcagta tacgtccttg agatgtaccg gcgggaggga
     9421 aaccccgacc tctggcacct gatcctgctc gccatgatcg tcgtcggcat ggcacccgga
     9481 atacgggacc tggtgcgcat ggtgctctat gtctgagggc tccctcgccc gggccgcggg
     9541 ccttgcggcc gccctctttg ccgtctcggt cggcctcggc gtcgttgccg tggcccgcga
     9601 cccggcggtc ggcacggagg tcatgggcct cttccgcgac caggtcgtag ccgaactcct
     9661 cgccgattcc ccggcggtcc ttgcaggaaa actgttctta aacaacctgg ccacctgcct
     9721 tctcctcttc ctcgggggag cctcgctcgg cgtggtcacg gcgctgatcc tctcggtcaa
     9781 cgggctcctg atcggtgcgg tgacggagct cgtgcggcag cagcagggcc tgctctatat
     9841 tgcggcggca ctcgtaccgc acggcatctt tgagatccct gccctcctca tcgcgggggg
     9901 cctcgggctc ctcctcgggc gtgagctcct cgcggaatgg cagggcgtcg gcgatgccgc
     9961 tgccggagcc ccgcccccgg ctcgcctctt tctccgggca gtcgtcccgc tcctcgcggt
    10021 tgcggcggtt gtagaggctt ttattacgcc ggcaatccta agtatgatag cttagagagg
    10081 cagatcgata gtttttgagg tacgagacgg atggaagaag atacaggcgt acttccccgg
    10141 aaagacgatt tttcccagtg gtacaacgag attctctggc gagccgagat catggacgtc
    10201 cgctacccgg taaagggtct gtacgtctgg tatcctcacg gcttcgccat acggaaacgt
    10261 gcatacggga tactccggga cctgatggac cgcgaccatg cggagacgat gtttccgctc
    10321 ctgatccccg agaccgagtt catgaaagag gccacgcaca tcaagggctt cgaggacgag
    10381 gtttactggg tcacccacgg cggcaagaac gagctcgacg tgccgcttgc tcttcggcct
    10441 acgagcgaga ccgccatcta cccgatgtat tcgctctgga tccggtccca caaggacctg
    10501 cccttaaaac tctaccagat cgtcaatacg ttccgctacg agacgaagca tacccggccg
    10561 ctcatccgtc tccgggagat cacgtcgttt aaggaggcgc acaccgtgca cgcgacacgg
    10621 gaggaggcgg cggcgcaggt cgaggtcgcg ctcggcctct accgggagtt ctacgaccgc
    10681 ctccgggtgc cggtgatcat ctcccgccgc ccgagctggg acaagttccc gggcgccgac
    10741 tacaccatcg cggtcgatac catcatgccc gatgggaaga cgctccagat agggacggcg
    10801 cacatgctcg gcgaccactt ctcccgcacc tacgatatca cctacgagga cacgaacggg
    10861 gagcggcagt atgcctacca gacctgctac ggcatctcgg agcggtcgat cgcggccaca
    10921 ataagcgtcc acggcgacga taaagggctc gtgctcccgc cggaagttgc tccgatagag
    10981 gtcgtcatcg tgccgatcat cgtcgggaaa cggcgcgacg aggtgctctc tgcggcctcg
    11041 gcgcttgagg tggacctccg gaaagccggg tttgtcgtga aactggacga ccgcgatatg
    11101 cgcccgggcg cgaagtacta ccactgggag atgcgcggcg tcccgttgcg gatcgagatc
    11161 gggccgcgtg atatcgacgc aaacacggtc gtcgccgtta cgcggaacgg caggaagacc
    11221 acgcttgata ggcgaggcgt cgttgaaggg gtctgctccg tccttgcaga gttcggggtg
    11281 gaactttcga aggcggcggt ggaggccgtc gccagccgga tcaccgtgac cgggaccctg
    11341 gaagaggctg ccgcagcggt gaagaccggc gttgcagtcg tgcactggtg cgggtcgcag
    11401 gagtgcgcag aaaagattga ggcggcagtg gatgcaagca tcctcgggtc cgacatccgg
    11461 tcggacctga tcaccgtctc ggacggccca tgcatcgcct gcggcgaaaa cgggacgtcc
    11521 gccctggttg cccggaccta ctgatcagca ggacgggcag gtatacgttt tctggtgttc
    11581 gggggacgaa aggtctcccg agatcaccgc ttttcctcct gagaggaata ttctaccgca
    11641 ctttgagcat ctcttcggga gcagtcgctc ccagcggctc atcggaactt ccagagaaaa
    11701 ctgcatcttc tccgcgtcag cgggattgca ctgggtgagc tcctcatacc gggagaggat
    11761 ctgcatgacc tggaacgggt ttgctccttc gttgcagaac agtttaaaga catagaattg
    11821 gaagatcatc cacccgaggt cggagcgctc gagggtctcc tcgtactcgc tgcacgctcc
    11881 gtcgtcctcg tcgagggagt acccgcagag gggacagcgc ccgcccacga gttcgtccag
    11941 gaagacctct tcgtggcagc tgggacaggt ggtgtggggg gtatgcattc tggaagtcat
    12001 gattgtactg gcctcatatc ttttactctt ccagggacac ggggcggggc cgcgtgcctt
    12061 ctgaaagcat tccacgcgga atgcgcggtc gctacgatat ctgaggtttt actacgtatt
    12121 gcgttcgtga tgctcttata cgtgtcgaat gcggcgcccg gatccactcc gtggggggtg
    12181 ttcgagatcc ggggatccgg gacggcgggt ggcgtcgccg gaagaccgga ggtcttctca
    12241 agctccggcc cgtcgcacct cgcacctaac ggtgctcaag ctcactacgt tcgcaccccc
    12301 aattcagccg ttttctcctg aaccgggccg gcggcataga acttttatat tttgccgggg
    12361 cactctgcgc gcatggcatc cccggcggca tctgaagtct acatcatcga tgcatcggac
    12421 cgcaaccacg ccgttgaagc gctctggcac gaggtcggcc tcccctcctt cgacgataag
    12481 acggtcgcgg tcaaggcaaa cttcaacagc gacgacccat cccgggatgc tggaggcggt
    12541 cctcgggcag gtccggaatg ccggcgcccg gacggtccgg ctcggggagc ggagcgggat
    12601 gggggagacc cgcacggtgc tgaagaaccg cggcgccgcc gacgtcgcgg cccgggcggg
    12661 gcggaggtgg tggcactcga cgccctcccc ccggaggggt gggaggcgat cccctcagac
    12721 ggcctccact ggcaacgggg tttcctgatt gcccggatct tccgggcggc cgatgcggtg
    12781 gtccagacct gctgcttaaa gacccaccgg ttcggggggc acgtctccct ctccctgaag
    12841 aacccggtcg gtgtcgtggc gaagagggat cctgcggacg gctacaacta catggcggag
    12901 ctgcattcgt cgccgcacca gaggaggatg atcgccgaga tcaaccggtt cttcccctgc
    12961 gatatcgcta tcctggatgc gacggaaggg ttctcgaccg ggggtcccga gcaaggcaac
    13021 cgtatcgctc caaacgtcat cctcgcgagc accgatcggg tcgccctcga tgctgccggg
    13081 attgcgctgc tccggcacta cggctcgacg ccggaggtga tgcggggcag gatctttgca
    13141 atggatacca tcgtccgggc ggcggaactc ggtatcggcg tcaagtcggc ccaggacctc
    13201 aggctcgtcg cgctcgactc cgagagtaaa gatctcgtcc tcgatatgcg gcggatcctg
    13261 gaccagactg cctgaagtat cagaagaaga gatgttcgag caggattcgg agcccgatgc
    13321 cgatcaggat cacgccgccg aggatctcga ccttctgccc gaatacccgg tcaagcgaac
    13381 tccctatcag cacgccggcg gcggagaggg caaaggtcac aacgccgatc gtgactgcgg
    13441 gccagaagat cggcgtatcc agcacggcga acgtgacgcc gacggcgagc gcgtcgatgc
    13501 ttgtcgcaac ggcgagcaga agcagtgtgg ccatgctccc ggtggcaaac tgcacgctct
    13561 cgtcgtcctg gcgcaccgcc tcgacgatca tcttcccgcc gatcagggca agcagcagga
    13621 acgcgatcca ggggccgtaa gccccgacga acgaggcgag agtcaccccg ccgagccacc
    13681 cgagcaccgg catccctgcc tggaacccgc cgaagagtgc tccgacgatg agggcatacc
    13741 tggcccgccc cccccgtatc gtggcgcctc cgctgatcga gacggcgaac gcatccatcg
    13801 caagcccgac ggcgatgagg agggttgtcg cgaactccat tgcttccgca tagatccgac
    13861 gcgatgccgg ataacgctat ggtcggcggt tgttatatgg cgcctgagtg ccctaccgat
    13921 acggagagat gatgaagatg agcacgcaaa cgatcggcct catccagacg gcggtgggcg
    13981 acgaccccga ccgcaacctg gcccgcaccc tcgctatggc agaacaggcg atcgcgaagg
    14041 gcgcccggat tatctgcctg caggaactct ttcgggtccc ctacttcccg cagtacgagg
    14101 atacggacgc ctccgcgtac gccgagacga tcccgggagc ttcgaccgaa gcgttctcgg
    14161 cacttgcccg ggagcacggc gtggtgatcg tcgtgccgct ctacgagcgg accgcgtccg
    14221 gcgagcacta caacgcggcg gtggtgatcg acgccgacgg acggctgctc cccgcctacc
    14281 ggaaggtgca tgttccctat gacccgctct tctacgagaa gacctacttt tcgcccggga
    14341 accgctaccg ggtctacgat acccggtatg gccggattgc cgtgctcatc tgttacgacc
    14401 agtggttccc ggaggccgcg agggcggttg cgctcatggg cgcggagatc atcttctacc
    14461 caaccgccat cggcagaatc gccggggagg agccacctga aggcgactgg cgtgaggcgt
    14521 gggagacggt gcagcggggc cacgcgatag caaacagcgt ccatgtcgcc gccgtcaacc
    14581 gggtgggcga cgaggggagt atccggttct tcgggagctc gttcgtcgcc gacgccttcg
    14641 ggaacgtcct tgcccgggcg agcgagacca acgaagaggt cctcgtcgtg gaggtcgact
    14701 tcgcagaaaa cgaggccgtc cgggagggct ggggcttctt ccgaaaccgt aggccggaga
    14761 cctacggggc gctcacccgg agactcccgc cgggccgaac ccctgcggga tccggctacc
    14821 gcatgccggc ggagtgggag ccgcacgatg ccgtctggct ctcctggccc catgaccggg
    14881 agacattccc cgaccttgca acggttgagg cagcctacgt cgagatgatt gcggcgctcc
    14941 gcgggtccga gaccgtcagc ctgctcgtca ccgacgagaa gatgcagatc cgggtgaagg
    15001 cgatgctcga ggaggagggc gtcgatacgg ggggcatcag gttccatgtc gtcgattatg
    15061 ccgacgtctg gttccgggac tacgggccga cgttcgtggt caacagaaag gccgggagcc
    15121 tcgcgatggt gaactggacg ttcaatgcct ggggagagaa gtacccggcg ctcatggagg
    15181 atacccgggt cccgctcttc atgaaccgcg agatgggtct ccccgtcttc acgcccggga
    15241 tcgtcctcga gggcggttcg atcgaggtga atgggtgcgg cacggtgatc acgacggagg
    15301 cgtgcctctt aaaccctaac cggaacccgc gcctctcccg ggaggagatc gaggcgtacc
    15361 tggaggccta tctcggcgca ggtcacgtca tctggttaaa gcggggcatc gccggcgacg
    15421 acaccgacgg tcatatcgac gatatcgccc ggttcgtgaa cccgacgacg gttctgtgtg
    15481 ccctcgagga gaacgaggac gacgagaact acgctctcct gcaggagaac tatgagattc
    15541 ttcgagcatc gaccgaccag gacggcaacc ccttaacggt cgttccactc cctatgccgg
    15601 ggagggtcgg cggcgcggaa aggctgccgg caagctacgc gaacttctac atcgggaatt
    15661 cagtcgtgct ggtgccggtc ttcgggcacc ccaacgacga ggtcgccctg gcacagatcc
    15721 ggcaggtctt ccccggccgg aaggtcgtcg gcatcgactg cacggcgatg gtcgcgggtc
    15781 tcggcgcgat ccactgcatc agccagcagc agccgtccat cgtgccggac gtcaccggcg
    15841 ccgcggcaga aagtgcatca cgagaggaat gagcgcatag gccgcggtgc tccaggtcat
    15901 tccggcgagg gcgagcagga tcgcgacgac ggagacggcc gggaccacca tcacccgcag
    15961 gttcccgcgc tgcatgtcgg tgccgggatg gacgagcctg ccattctgcg tggcgtaccg
    16021 ccactgagcg gcaaagagca ggccgaggat caggaggttt gcttcgaagg cgactgctgc
    16081 gaaggtctcg tccgggtagt cgccgacgag ggaggtggag aacggcacca gcgtgacgaa
    16141 caggagcgca acgatattga tccagaggaa ccggctatcg atatgccgga ggcggtgcat
    16201 ctgcacatga tgcgctatcc agaaggaagc gaggatgaag aaggcgagga agtagtggta
    16261 gagggcggga ccgagattgg taagcatcgc catgacggcg gcgtcggagg ggttgttgac
    16321 gcccgccggg acgtcgaggc tgaggaccag gagggtcatg gcaatggcga aaatgccgtc
    16381 ggtgagcgct tcgattctgt tcttcggaaa tccgggtgat cttccgctcg cctggtcggc
    16441 gtcgtccgtg ggctccatcg ctaacgatct ctattgggag ggccggcctg ataaggagtc
    16501 aactaccccc ggttaaaacc gggggcttgc agcgggggcc cttgtggggc acagggctgc
    16561 aatttaatcg gtgcgaaggt tgacggttca aaagacgtcc ttattcgcac gggccggttg
    16621 acgcggcctc taccgattat ccgtgggatc acggtatcac cggctacctt tcgtaaatgg
    16681 tttagagcgc cgttgacatc ggcgttgatc agggtgcc
//
LOCUS       DAJB01000115            5917 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_83119, whole genome shotgun sequence.
ACCESSION   DAJB01000115 DAJB01000000
VERSION     DAJB01000115.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_83119"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1125 a         1912 c         1837 g         1043 t
ORIGIN      
        1 gcgatatgca tcccgatacc gccgccgccg gcactcgcct tcacgatcac cggatacccg
       61 atctcggcgg cgaccttctt cgccgcatcg acgctggtga cgccttcggg cgtgcccgga
      121 aggaccggga cacccgcctc gcgcatcatc tgcttcgacc cgatcttcga acccagcgcc
      181 tcgatcgtct tccacgaagg ccctatgaac gtcaggcctt cgtcctcgac cagcttcgca
      241 aacccggagt tctcggcgag gaacccgtac cccgggtgaa tcgcctcggc cccggtcttc
      301 ctcgccacat cgcagatccg ctccttgttg aggtaacttt tggacgggtg cgcctccccg
      361 acacagaacg cctcgtcggc gtacttgacg tggagtgcat tactgtccgg ctgggagtag
      421 atcgcgaccg tgtcgatacc gagctcccgg caggcacgca tgacccggat ggcgatctca
      481 ccccggttgg cgatcaggat cttatcgaag tatttcatcg cctaaacagc cctcactcaa
      541 tgaccatcag cacgtcgccg ttctgcacga cgtccccggc atccacgaag atctccgaga
      601 ccgtgccgtc acggggactg cggatggggt tctccatctt cattgcctcg aggacgatga
      661 gggtatctcc cttcttgacg gtgctgcccc gcgggaacat cactttgagg accatgccct
      721 gcatgttgct cttgaccccg ccggcgacct ccccgcgggg tatcctctct ttggccgacg
      781 gagcgctcac cgaagccccg accgagtctc cctcgacggt gatgatccgg accgagaaga
      841 tctcgccgtc cacctccacc tccatggagc gggggaagtc tgcaactccc accggtccgg
      901 cgggtgccgc cgcttcaggg atcacttcgg cctggcgctc accccggagg aacgacggag
      961 cgatgctcgg gtagaggatg taggtgagaa catcttcctc tttggtgacc agaccctgct
     1021 ccaccgcctg cgctcgcatc tgctcgtaga tgggctcaag aaggtccgcc gggcggaccg
     1081 tgaccggctc ctcgtcgccg acgatcaacg cccgcacctc ggggctgatc ggggccggag
     1141 agcgaccgta gaggccgagg acgtagtcct tcacctccct tgtcacgttc cggtagcgct
     1201 ccccgtccat gaggacgttg aagaccgcct gggtaccgac gatctggctc gtcggcgtca
     1261 cgagcggcgg gtagccgagg tccttcctca cccggggaac ctcatggaac acgtcgtcca
     1321 ggcggtcgag cgcattctgc tcctttaact gggagacaag attggagatc atgccgcccg
     1381 ggagctggta gatcagcacg tcagagtcga cccggtccgt gatcgcggag aagagcggtt
     1441 cgtacttctc ccgcatctcc cggcagatgt tcctcacatt ccggagcgtt atcaggtcga
     1501 taccggtatc gcggggcgtc ccgctgacgc ttgcaacaac gctctccgtc ggaggctggg
     1561 aagtcccgaa ggcgaacggc gacatcgccg tatcaaggat atccacgccc gcatcgatcg
     1621 ccgcctggta actcaggggc gcgacgccgc tcgtgcagtg ggagtggaga cagaccttga
     1681 cgtccgccgt cttcttgatc ccggcgatca ggtcgcgggc atcgtgaggc atgatcaggc
     1741 cggccatgtc cttgatgcag atcgagtcgc agccaagcgt atagagctcc tgcgccatat
     1801 cgatgaacgt cgccacggag tggaccggac tcgtcgtgta ggatatcgca ccctgcaggt
     1861 gcgccccgac gttcttgacc tcctccatcg cctttttcat gttgcggata tcgttcaacg
     1921 catcaaagac ccggaagata tcgaccccgt ttcgtgccga cgcctccacg aacttctcca
     1981 ccacgtcgtc gggatagtgc cggtagccta cgaggttctg gcctcgcagg agcatctgga
     2041 tgggagtgtg cttcagctcg gccttcagca tccggagacg atcccaggga tcgtcgttga
     2101 ggaacctgat gcagctgtca aaggtcgctc ctccccaggc ctcgacagag aagaaacctg
     2161 ccggatcgat ggcccgggca agagggagca tgtcctcagt ccggagtcgg gtggcgataa
     2221 gcgactgatg cgcgtcccta agcgtggtat cggtaatatt gagtgaatca aatttggcag
     2281 cacacatcga attttgcacc tcgaagggct gagaagatat cgataaagcc tctaaaaaat
     2341 tcatcctact aatataaaaa cgtcacgaag aggcagacag gataaaagcc cgggaataac
     2401 ggctgtcagg aaagccggaa ggtctcgcga atctctcctg aaccgggggc atatctggcc
     2461 tccgatcgtg tatccccgca ccacaagcag ccgggctatc tcgtcggacc gccggatctg
     2521 cgtgacgatg agcgtgacgg cgagcggcac gatcgagcgg gcaccgggcc ggattccctt
     2581 cagcgccatt gcaacccgca tctgctcaat ctcctgccgg atcacggcga ggctcgtaag
     2641 ccccatctcg gcgatgagcc cgatctcgaa ccccacccgg tttccgaggg cccagaccgc
     2701 cacggcaagc acctcgccgt cctctgtccc ggcatacgtc catgcggcga ggaggaggag
     2761 cgcgagcatc cggatcaggt aggagagccc cgccccgccc ccccacgcgg agaggagcgc
     2821 tgttgcggcg atcatcgcga cgagggggag aaacaccccg ggacgcggaa ggaccttcgt
     2881 gcgcggcgta aaaatcagcc accaggccag gacggcgacg gctcccgtcg tgcttgcaaa
     2941 cgcagcgagc gagaggatca cggcggcaag aagcctcagc cgcggatcct gcatagcgcc
     3001 tcccgggcat cggtcagtct gatattctcc gggcaggccc cctcttctaa ggcgtacctg
     3061 aggtagggcg gggcgtgccg ccaccgcgga atcgcaccag gaacgctgcc gcaggccgtc
     3121 aggctcccgg cctccatctc ccagagggca tccacccggg ggaggaccgc gcgctcgtgc
     3181 gagaagatga tggtgatccc ctcatccctc tcttcgacca tcctgcaggc ctccctcttc
     3241 gcggtgcagt ccagggagct gaacggttcg tccagcatca gcaggtccgg gttacgcatg
     3301 agcgtgcagg ccaggaccaa ccgtttgagt tcgccgcggg agaggtggaa cgggtcgtcg
     3361 tccccccgcc ggagaaggtc cgcctgcgcg agcacgtcct ccggcataag tccccaggac
     3421 ctgatctctt cggcaacggt cgggcaggtg atctggtgtt cggggaactg cagcaggagc
     3481 agggccgacg agacgttgtg ccgccggacg gcgccgcgaa gaggaaggag cagttccgcg
     3541 agcagaaggg cgagcgtcga tttcccgctt cccaccggtc cgctgaccag gtgcacgccc
     3601 tcttcaaagg tgccgctgcc ccggaggctg aacggaccgc gggagaagag gaggtcctcg
     3661 aggtcgaccc tcaccggcgg accctccaca tcgtggggta gaagcacgtc tcctcgagcc
     3721 tcgcgaagac ctcttccggc gtgccgtggt ggcggaccac accaccctcc atgaagagga
     3781 gatagtccgc acgagccgcg gtatccatcg actgcgtgca ccagagcaca tgggccgccg
     3841 tggcctcctg cacgacacgg ccgacgcgct ctgccgtcgc gccgtccagg tgcgagtcgg
     3901 cctcgtccag gacgagcacc tcggggtcgc cggcacaggc cgcagccagg gcgacgaggg
     3961 ccttctcacc cccggagagg gccgagacct tcgcatcaag gaggtggggg atgccgaccg
     4021 tccctgcagc cgctctaacc cgctcatcgg tctccctgca gggtcggtga cggaaccggg
     4081 gagtcgacgc gatctcgtcg tatacccggg agaagaggag cgtccggtcg gggaactccc
     4141 ccacccaccc gaccttcacg gcagacggcg gtctcccgag gagccggacc gtccctctcc
     4201 cgggctcctc gatgccggag cagaccgtaa gaagcgtcgt cttgccgctc ccgttcggcc
     4261 cgatcacggc gatgtggcgc gaatccactc tcagatcggg gatatcgagc agccggtgcc
     4321 ggagatcggt gatgcggatc atgccacccg ctttcctatg gtgtacgcgg ctgtaacctt
     4381 cacgacctcg ccgacggcga acggggcgac cccgagcagg accgcctgaa caatcgagag
     4441 cgatgccgac caggcgagcc atgcgacgcc tgagaggtag atcacgccgg tcgctgcgat
     4501 caggcctgcg acccggcatg ctgccgtcgg ccgttcatag gcgagcccga cgatgagggc
     4561 ggcagggatg aaaccgagca ggaaccctcc cgtgggcccg aggagcaccc cgaggcccgc
     4621 catgccgtta tgaaagaccg ggaggcctgc cgccccgaag aggacgtaga gaccgacggg
     4681 gatcacggcg taccgcttca tgacgatccc ggagaggagc acgaaaaggg tctggagcgt
     4741 gagtgggacc gggggcagcg ggatggagat ccagctcccg gcagcgatca gcgcaatgaa
     4801 cgtaccgctg taggcgagga tccgggcacg gtgtatcatg cagacagata ggttggaggg
     4861 ttgatgataa aaatcctgtc aaccagaaaa tctggagtgg ttgacaggac ccggaggctc
     4921 tagagctgga aacagtcgcc ggcgataacg cgctctacct tcccgttatc cttccggaca
     4981 atcagggcgc cgtggtcatc gatatcgatg gcctctccct cgaaggtctt gttgacggtc
     5041 ttgatggcca cccggtggtc gagcgtcagg gagaggctct tccactcacg gacgatggaa
     5101 tcatactcgc catcctcgag ctgctgatag cgcagttcga actcccgcag gatccgggca
     5161 agaagcgcca cgcggtcgac cgggtggccg acctccgcct cgattgaggt cacggtctcc
     5221 tgcaggttcg gcgagagttc tgcaagagag atgttcgcgt cgatcccgat cccgaggagg
     5281 ccgtaatgga ccgtatccgc ctctgcagca agttccagga gcagcccggc gaccttctta
     5341 tccccgacga agatgtcgtt gggccacttg atgagcacgc tgatgtcgta ctccttgcgg
     5401 atagcacggg caatcgcgac cgacccggcc atggtgatca tgaagaggtg gtcgagcggg
     5461 accttcggtt ttaagatgat ggtggcccag atgccgccgg caggcgagac ccaggcgcgt
     5521 ccaagccttc ccaccccgcc ggtctgctct tcggcgatga tcaccatgcc gtgcagtttc
     5581 tcagggtcgg tctcgccggc aagtttcttc ccgatccagc tcgtggaggc ggtacggtcg
     5641 aagtggcgga tctgtcgtcc gatgaaccgg gtacggaggt gcttatggat ctcgtacgga
     5701 aggagcctgt cggtcttcat ctcaaggcgg taaccttccg cccgcgacga ggatataccg
     5761 tatcccagcc tccggagttc gcgtatctgt ttccagacag ccgatcgggt gataccaagt
     5821 tgctctgcaa tccgctcgcc cgataccgga ccaggcgatt cttcgagaat ctttaatacg
     5881 ttgattgccg tatccttcat aataatcagg caggaga
//
LOCUS       DAJB01000116            7109 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_94178, whole genome shotgun sequence.
ACCESSION   DAJB01000116 DAJB01000000
VERSION     DAJB01000116.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_94178"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1352 a         2395 c         2156 g         1206 t
ORIGIN      
        1 cttcatggca tataaacatt acaccccatt ccgggatttg gcgcgaaaat tccagaagaa
       61 ttttcgcccc attacagatc ccggccggaa tcgaagagtt ccgaccgaac ctgcatcacc
      121 cttgtgagac ataatattgt agccaatcgc atataaacat tatgttctgg cccggcattc
      181 atcccggcgg accggaataa atcccgaaca gatgacggaa caggccggaa tacaccgccc
      241 cgaaccatct cacccttgtg agttgtaata tcagtaaccc cgagtatata ataattgtgc
      301 aaaaatgaag gttaaaacag aacattctcc agagaatccc ggcatcccgg gcctgacacc
      361 cgccctttca tcgccagatc gggatcccgc gcatgataac cgccgcgccg gcatccccgg
      421 atctgccggg gagagagcgc acagaccatc ttactgcacc cgcatcggac agagccccgc
      481 tgtggaggcg agtgcgcgtc atcttcaggg gaaactacag gactgatcgc ctgcccggct
      541 gcccggttgc gcgcaggacc cgatgcccgc gcggggtgaa ccgcgcccgg gccgataacc
      601 cacacgacgg gttggcccgt gctccgtata gaacccaaaa gaacccatat gtaacaactg
      661 ttttcccacg accgcacaaa caaagagtca caaccgggag ggctcccgac cctccctgac
      721 ctccgagacc gcccatgccc cgcttcaacc tcagcccgtc gctcatcggc tggttcttct
      781 accacgactg cgaacgctac ctccgctatc acgcgacccc ggagcaggag cgcgaaatag
      841 ccggcatccc ggcggttccg atggacagga acccggtgac gcgagccctc ctggatgcag
      901 gcatccgctg ggaagaggag gttgtccgca ccagactggc aggagcggtg cgaattccgg
      961 acgggacagg ccccctctcc gaccgttcgt tctccatcga ggagagtttc gacctcctcc
     1021 cccggctctc cccgggcgag gcgatctacc agtcgaccat cccggtctcg gtccactttc
     1081 tgcgcaacta cggtctcgaa cccggggtgc accggttctc cccctgccgc cccgatctca
     1141 tcaggcttga tgaagagggg tgcctgcaga tcatcgacat caaggcgagc gaagacctca
     1201 gcgtcagcca ccggatccag gcaacgctgt atgccctgat cctcgatcac gccttaaacc
     1261 tgctcgggat cgatctcaaa gtcgacctct cccgggccgg gatctggctc tccggagcgg
     1321 acgacccgga accgttcagc ctgcacctca acatacgggt gatcgaggac ttcctccgcc
     1381 accgtctccc gggtatcctt gccggcccgc cggaggacgt gccctggcat atcacctcac
     1441 ggtgcgagtc ctgtgagttt tactcccact gccgcgagga ggcggaggcc tccgcctcgg
     1501 tctcgcagat accgggcctc tcgacggtcg ggcggcgtta cctccgcgaa gcaccctgga
     1561 gcgccaatct ctccatcaac acgcttgccg acctggagag gtttcttcgg gaccccggga
     1621 gcgaccggca cctcgacaac tgcgggtcgc tcgccgacca gggcgaccgc ctgcgggcaa
     1681 ccgtccgggc gctcaccacg ggagaggtcg tcgcgctcgc cgcaacctcg cttgccctcc
     1741 ccatctacga agacatcggc atcatcctga cgctccagaa ggaccccgtc tccagccagg
     1801 tatacgcgct cgggttccgg cggagcaagg gcgaagcagt ctacgggacg ccttcaaacg
     1861 aagccgtctt cgttgccgcg caccctgatg actgcacgcg ggtgcggcag gagtttgtcc
     1921 gggcactcac cgcagaactt gcggtcgtcc acgactataa ccagggcagg gactgggcgg
     1981 accggaagtc ggtgcagacc tacgtctacg acacctacga gacggaactc ttcacccggc
     2041 tgctcgaaga ggcgataaaa gaccccgcga ccgcggagga tgccctgcgg ctgcggttct
     2101 actaccagga ccccggggtc gctctcgggt ccagccaccc gagcagttcg gtcccgttcc
     2161 cgcttgtcgt cctcacccgc gagatccggc ggctgcttgc gctccccatc ccgttcacgc
     2221 tccgtctgcc ggaagtcctt ctcgccattc cctcttcgcg cttcgcatac cggctgaccc
     2281 cggcgagcct cttctggaac gagcagagca acgcgatgaa gagcgacgcc atcatcatgg
     2341 cgtggagcgg ggcaagaccg gaggctgcag gctgggtccg gcaggagata tcgcgccgcc
     2401 tgcttgcggc cgggagcgcg ctcgacgggc tccgcgaacg ggtgaagggc agcctcgtgc
     2461 ggtgggcgga gaacttccgg ttcccgcgct cgtgggatgc cgccatgccg gagatatccc
     2521 gcctcctctt catcgcggag tacgaatcga ccatgggtgc gcggcggata caggaactcc
     2581 ggagcaggcc gtggaccgta agggtccggg acgggacaag catccctgtg cggaagagcg
     2641 aggggaactt ctggaaggtg ctcgcacctc tcgacctctc gctcatcgag cagtcgcagg
     2701 cattcagtta cctcctcgtg ccggacggcg atgcgggtga ggaggccctc cttgcgttcg
     2761 acgacctccg ctaccggagc agcccgaacc cggggaagag tggagtctgc tttgccaggg
     2821 tccgggacag catcgtcgac cggaaggccg gacaggtcag ggggttcgcc cttgaggtga
     2881 cctactgccg ggagcacgca cccttcgccg agggcgacct cgcggtgctg cacccccggt
     2941 tcaccgattt tgtcgtgccg cgatacgtcg accgccttct caccctggac gagcagccgg
     3001 agaacgcgtt catccgcctc ctccgggacc cgcaggggtt cgccgccccg atccgtgagc
     3061 caggggaggt cgtcgccgat gcagaacggc tcgtccacga cgccgggttc actaaaagcc
     3121 aggtccgggc gttttcccat ctgatgacga accgcctgac cctcgtctgg ggaccgcccg
     3181 ggaccgggaa gacgcatttc ctcgcgaagg ccatcctctc gctcgtcaaa gcccggaggg
     3241 cgcacggaga gcgtatcagg gtgggtgtcg cggcattcac ccacgccgcc gtcgagaacc
     3301 tgctcatcaa ggtgcaggag tccgtcgacg agttcgacct tgccgcaggc ctgccgatct
     3361 acaaactaaa gagcatcagg acgcccagag gcgaacggag cctcgaagtg ctgccccacg
     3421 accgggccga gaccgtcgtc ggctacccgt ccctcctcct cggcgggacg gtgcacagtt
     3481 tcggcaaact cgagaagtcc ctcccgtccc tcgacctcct ggtcgtcgat gaagcgtcgc
     3541 agatgaggcc ggccgagctg gcgatggtgc tcccgatgct cggccagggc ggaaggctcg
     3601 tcctcgcagg tgacgacctg cagctccccc cggtcgtcca gggggagtat ccggtgccgg
     3661 aagacgggct gccggggctc gatgactcca tcttcgccta cctccgccgc cgtgacgacc
     3721 caaaacaccc catctatacc tgccagctcc aggagaactg gcggatgaac cgcaccctct
     3781 cagggttccc ggcggagacg ctctacggcg ccggctacgc tccggcgtcc gacgcgatcg
     3841 ggcggcagcg gatcgccctt ggaccaggtt cgcccgagga ggagtttatc gagtggatcc
     3901 tcgatcccgc gtacccgctc gtgctctgtg tcctcgaggg cgtccggacg acggtggaga
     3961 accggatcga ggcggcgctg gttgcccggc tgaccggagc gctccgggag aggctcctcg
     4021 acccctgctc cggtaagccg tatccggcga cagaggacgg ggacttcctc ttctggcggc
     4081 aggggctctt catcgtcagc ccgcaccatg cccagatcgg ggcaatccag gatcacctgg
     4141 ccgacattcg agcctgggaa gcacacccgt tcgtggacac cgtcgacaag atgcagggcc
     4201 aggaggcgga ggcggtcatc gtcagttacg gcgtaagcga tgtcgagacc gcccttgccg
     4261 aggccgagtt catctacagc agaaaccggc tgaacgtatc gctgacccgg agccggtcca
     4321 aatgcatggt cttcctgccc cggccgctcc tggagccgcc gctcgacctc gtgcagaacg
     4381 agaaggcggc ggtgggcctt acgcatatgc tcgacctcca ggagttctgc cgggcccacg
     4441 gtgaagagcg aacgttcgac ctcgacgggg cggcaggcgt ccggctcacg gtgatgcggg
     4501 cgcgggaagg gtgaagagag cccgcaacag tgatctcccg gatctagttt ggtgtctttt
     4561 ctccccacct gtcctgagat agtggaccgt gggggtatcg ccacgggggg aggggctgac
     4621 ggggagggtt acccctcccc tgtctccacc aatctgggaa atatataacc cccaccccgc
     4681 ccggcctccg gcctcctccc ccgccccagg gggcgggggc agtgcgtggc gataagcgac
     4741 tggccgaagg gcaggagcag gagaagacct tcggtcttag actggtgacg ttccgacagg
     4801 aacggatctc gagcaccgcc aggtgcgcag cccgatggaa atccgtgcca aatattgcaa
     4861 tggcataaac acgaccagcg caattcaaga ggccctcaac ccccgggccc aacctatttc
     4921 tcaccgtcag acagaatgat ccctgtgatc ggcaccctcg accccgacca gaacgaccgc
     4981 gaacgccggc ttctcgacgc tatccgcgag cagggatcaa caccggagac catccgcctc
     5041 tttcgcgacc tcatcctctc ccactaccgc gcccacggcc gcgacctccc ctggcggcgc
     5101 accaccgacc cctaccggat cctcgtctcg gagatcatgc tccagcagac acaggtcgag
     5161 cgggttgccg tgaagtaccg ggagtttctc gaacagtttc cggacttcga gagccttgcc
     5221 cgtgcaccca gaagcgaggt gctcctcgcc tggcagggca tgggatacaa ccgccgggca
     5281 atcgcgctcc agactaccgc cgggcgcgtg gtggacgggt acggcggccg gctcccggcc
     5341 gatcccgata cgctcgcaac cttccccggc atcgggaagg cgaccgcggc ggcgattgtt
     5401 gcgtatgcgt tcaacatgcc ggtcgtctac atcgagacga acatccggcg gatcttcatc
     5461 cacttcttct tccaggaccg ggaagatgtc cgggacgacg agatactccc cctcgtcgag
     5521 cagagcctcc ccggggagaa cccgcgggag tggtacagcg cgctgatgga ctacggcacg
     5581 gtgttaaaga agcggacggc gaacccgaac cggcggagcg cgtcctatac ccggcagagc
     5641 cggttcgagg ggtccgaccg gcaggtcaga ggacgggtac tcgcgctcgt cctcgaggaa
     5701 gggaccgtca cggaggagca ggtggtgctc cgggtctgtg agaatcccgg cagggtgcgt
     5761 aagatcctcg gcgacctgga gaaggaaggg ttcatcgccg agagcgacgg ggcgtatacc
     5821 tgcaggtgag aggcggcagg ttgataccgc tacagtctca ccccgggaaa ggcatgaaga
     5881 tcggctaccc gtgcatcaac cggagcgtcg gctgctcctg tggccgcacg ttccggctct
     5941 cctcctactc cgatgaacgg ctggaagaga ccgtcagaga gaacctcagg tgccttgatc
     6001 taatcctccg ctttaaccgg agagagtcca tcctcttctt ccggatcggc tcgggtctcg
     6061 tccccttcgc atcgcacccg gtctgcaccg ccgcctggca ggataccttc gccgggacgt
     6121 tcggggagat tggccggttc gtcagggacc acgggatgcg gatctccatg caccccgacc
     6181 agttcgtcgt cgtcaacgcg aaagacccgg ggatcgttga gaagagcgtc gcggagctcc
     6241 gctaccacgc cgcggtcctc gacgccatgc accttgatac gaccgcgaag atccagatcc
     6301 acgtcggcgg cgtatacggg gaccagaagg agagcatgga gcggttcgca agcatatact
     6361 cccgcctcga cgagagcatc ctccgctggc tggtcgtcga gaacgacgac tcgcgctaca
     6421 cgctggccga ctgtctccgc atccacgagg agaccggcat cccggtgctc ttcgacgtct
     6481 tccaccacca ggtcaaccct tcgggcgtcg gggcggccga agcggcggcg cggtccggcg
     6541 caacctggcg gcttaaagac ggcatcctga tgaccgacta cagcacccag atgcccggcg
     6601 gacggagagg ccggcatgcg gagagcctgg accccaccca ttttgagcag tttctcgccg
     6661 aaaccgcgtc gacggatatg gacatcatgc tcgagatcaa gggtgaacta ccccgccctt
     6721 tagggcgggg cttcctgctt cattctcccc ctattgaggc gagtccacag gctcttcggc
     6781 gcgttccgca cctgctactc cgacaaggtt ctgctcttgt agggcgaact tcttgatatt
     6841 gatcgctgcg ttactatctc aatcgtgaac tgtaccacag tcgggacacg cccattcgcg
     6901 atccgacaat ttcagatccc ggttcatata cgacgggcaa ggagaccccc tgcgcaagcg
     6961 gggggaggaa ttgcccgcat caagccccgt atactttcgc cccgcgggag ttctcttata
     7021 gacagatgag caccaaagat aagagataat ggcactgcga gtcgtcagca actatctgcg
     7081 ccttccccag aagacgttct ggatcctcg
//
LOCUS       DAJB01000117            8332 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_34732, whole genome shotgun sequence.
ACCESSION   DAJB01000117 DAJB01000000
VERSION     DAJB01000117.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_34732"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1669 a         2348 c         2551 g         1764 t
ORIGIN      
        1 cggtttcaac cgggggtagt tgaccgttta ccactacgtt tgaaaattat aagaacactt
       61 ttgggttata tgaatataca acgtctttat acataaacgt cgatattatt aattagtcaa
      121 tatgagggtg tgaagctgaa tgagggtctt cttcatagga ttcggtcagg cggggggcaa
      181 agttgtcgat atgttcatcg aacaggacaa acgaatgcag acccagagtt tcaggggaat
      241 cgcagttaac acggcgcgta ccgacctgat ggggcttaaa aatatcgagc tcaaagaccg
      301 gctcctgatc gggcagacgg tggtcaaagg ccacggtgtc gggacggata acgtaaccgg
      361 ggcacgggtg accgctgatg agatagatgc catcatcaac gctatcgact cgaggggcac
      421 ccatgatgtc gatgccttcg tcatcatcgc cggtctcggc ggcgggacgg gttccggggg
      481 ttcgccggtc ctggcccggc acttgaagcg tatctaccgg gaaccggtct acgcgattgg
      541 tatcctgcct gcgccggagg aaggccgact ctattcgtat aatgcagccc gttccctgag
      601 caccctcgtg aacgaggcgg ataacacctt catttttgat aacagtgcct ggaagaatga
      661 aggagagagc gtcaaggatg cctataaccg gttaaacgac gagattgtcc gccggttcgg
      721 cgtgctcttc cgtgcaggcg aagtcgggaa ggcgggcgtc ggggagatgg tcgtggactc
      781 aagcgaggtc atcaacaccc tgcgtggtgg cggcatcagc accgtcggat acgccatcag
      841 tgaaaaagtc ggtccgcgtg tgaagcagag tcaggggttc ctctcaggcc tccggcggaa
      901 gaaggagaag accgaggagg tgctgaccgg agaggacaag tcggcaaaga tcatcgctct
      961 cgtcaggcgt gccatgctcg ggcgcctcac cctgccgtgc gactactcga cggcggagcg
     1021 ggggcttgtt ctcctcgccg gcccaccgga tgagatggac aggaagggcg tggagaagtc
     1081 gaagagctgg gtggaggaga acatcgccgg tgtcgaggtg cgtggcgggg attacccggt
     1141 gcagagcgac tacatcgctg cggtggttgt tcttgcgacg atcgggaacg ctccccggat
     1201 caccgatctc cttgagattg caaagtccac gaaggaagat gtccttaaat cgaaggaaaa
     1261 gcgttcgacc atgttcgatg acggcataga gccgctattt gagtgaggag acgttatgaa
     1321 agcaagcatc agcagatgga tcattctcct gctggcactg gtctgtgtcg tgcaggtggc
     1381 atcggctttc gacatcaaga ccgagaccgt caaccccgca agcggcgctc tcgaacccgg
     1441 ccagcaggtg accgcgcgct acgtgctcac ctacgacatg atcaccaatg gcggtaacga
     1501 cgagacattc gaactcagca ccggattgca gaacccctcc tgggagttca tcgtctaccg
     1561 cgatggggtt gccatctata ccaccaagaa gactggattt tacccgatcc tgaccgagtt
     1621 cgagatcgac tatggcgatg aggatatcga actggacgtg cagttgcgcg gcatcgtccc
     1681 ctcgagcgcg agcggaaaga ttctggtgac caagatcgag cacctgcggg gtgatgacgt
     1741 gaaggacacc cattccgtga gccgcgacgt tgtgagttcg gatcaggtcg cgggctctct
     1801 ctccgcccag cagcagcgcc tcaaggatct gaaggcagat atggatgaaa agaccacgca
     1861 gggcgtggac gtctctgctg cccaggccaa gtacaatacc gccagtcagg cgctcaccag
     1921 tgctgcgtcg gcaggtccgt cacaggcgac gagttacatc gccaccgcca agaaagatat
     1981 agacgaggca attgcgctcc ttgataaggc atgggccgag aaagcagtcg ccgacaccgg
     2041 cgcgacgatc gagtcccttg atgggatgat cacctacttc gtcgagaacc ggtccatgaa
     2101 cagcgacccg caggtggttg ccatcatgac caagcgcgag agcgctgtcc agttctattc
     2161 ccaggcgcag gataacctga atgccaaaaa ttatccgctg gcgcgctcaa aggcggctga
     2221 cggcctgagt aaggcgaacg aggctttgac cgatgcgaat aatctgaagg agaagatcgg
     2281 cgaggggttc aacttcggcg gtaacctcct cctctacatc ggtgtcgggg tcatccttgt
     2341 cctcgttgtc gtgggcgttg tcttctaccg gaagaagagc ggctgggacg agctcggata
     2401 accccggcga caaaccctct ttttttcaca tccctctatt gctcaatctt cccttggtgt
     2461 cgtgcctggt gtactgcttc tcccgggacg cgctctatcg ttatccaggt ctcctgaggt
     2521 tcctctggcg gggggctaac tccctcatga cctttagttg cccatggtct gatattccag
     2581 gcgattaata ggtccgggaa gttcccggtg ctgagaacgc ctcttcacga tggcgacggg
     2641 caggaggtcc ccgccctcag ccggggccgg ttgacgctgc aaaaaagaat cgctggtcca
     2701 cgctctctac gcggtcgtcc ctgcggggac cctggagaat acctcgaggt ttctgctccc
     2761 cgatttccca tcccgcagga agtagtactc gagcagcctc tctttgttct catcgtacga
     2821 gaaccagtag ttcagccggc tcgtctcctt cacgtagccc tccagggttg ggtaggtatc
     2881 gccatcgtgg gtgagcacca ggtcaaagtc ccctccgtat atcgtcggct cgctcacctt
     2941 ctggctgtag taactgagct ttgaggcgcg ttcaccccgg taataccagg gcagcggcca
     3001 gtaggagtct gtcgcgaccg ccactttgtc cacggcatcg atcttcgcga agacctcccg
     3061 caggtcttcg gagttctgca cctgcacgat gggttcgttg atgtctgcag gggtgaacac
     3121 cacatggagc gtcatggcga ccaggaagac gcttgccacg acggcgatga ccgccttctt
     3181 cgtggtcatc aggtagaccg caacgaagat catgggcagg agctggtgaa ggatcagcca
     3241 cggcaccttc tcgccgatgt aggcgtagac cgcaagtgag agaagcatcc agaagatggc
     3301 gaaccgtgcg aactctttct gccggtcgat cggttggatt ccctccccgc gggagaagaa
     3361 ctcacggagc cggtctttcc agccccctgt ctccttaaag gaggggggtg taacctcctg
     3421 ggtctcctct tcctctggaa cgtccccgta atatacgctc tgggctccaa cctccgggac
     3481 tccctcgtga gatgcccgtc gctccttcca gcgggagacg atgccgggga tgtcgatgaa
     3541 ctgcagagta ccgaccacgg cgaggatcag gatcggcact tcgtagagga tgaagagcag
     3601 gatgtagaag tacggcggac caccgaggcg ttgcgcctgg tgcatcgaag tccagtgctc
     3661 gattgcccgg agccacccgt ccatgagcac ctccggatga gcgccgaagg aggagtagag
     3721 gaccgccatg atcccgaccg cgacgaatgc gccgagcgcg agatcccgga cccagtgagt
     3781 cggcagtcgc accttccttg tccagacaag gtagaggagg tataccccga agatcaggac
     3841 gatgatcggc atgttctcct ttgcggtcat cccgagaccg atcgccgctc cggcgatgag
     3901 tgcataccgc atcttctgcc gctcgaagta gtagaggagg gcgacgagga gcaccatcga
     3961 aaagaaggcg atgaagatgt cgtttcgcag aaaccgtgag aaatagacca tgtttggaga
     4021 tacggcaagg aagagcgccg ctatcagggt ctgcttctta tcgagatagc cgagtttata
     4081 gatcggatag agcagcggga cgatcagggt gccgagcagc gccggaatga gccggcccac
     4141 gagatcggag tccccgagga gcgagaagat cccggccgtc acgtagtaga ggaacggccc
     4201 atggtacatg ggatcgtaac tatagacccc ttcggtcagg agtttatacg aaaaccacgc
     4261 gtgaatagcc tcgtcgtggt gaaagagttt caaatcaagt gcgtaaaaac ggagagcaat
     4321 tgtgaccaga agtatgagga gaaaaaaccc ctcgaatgag agttgttcac gggcccgtgc
     4381 cgcgaacact gcggccttca cgccgctctc acctcaactc tccagcacaa tttcaatgcc
     4441 gatgtctttt ggaacctgaa tgcgcatcag ctggcgcagt gcacgctcgt cggcatcaat
     4501 gtcgatgagt ctcttgtgca ctcgcatctg ccagcgatcc caggttgcgg taccttcgcc
     4561 gtcagggctt ttcctggtgg gcaccacgag cttcttcgtg ggcagcggga tcggacctgc
     4621 cagattgacg ccggtgcgct ctgctatctc tttgatctta ttacagacca tctcgagttt
     4681 ttcgaagtcg gttccggaaa gacgtattct ggccttctgc atgtttaacc accaaaaaat
     4741 tataggtacc ctattatctc atctgcttgg gggtgatgtt gatgcacaca cctgccgcaa
     4801 tggtcgatcc catatcacgg acagcgaacc gtccgagctg cgggatctcc ttgaccttct
     4861 cgatgaccat gggctgagta ggcttgatct tgacgatcgc ggcatcgccg gtcttgagga
     4921 acgtggggtt ctcttccttg acctgaccgg tacgggggtc gagcttcttc tggagttcga
     4981 cgaaggtgca cgcgacctga gcggtgtggc agtggaagac cggggtgtag ccgacggtca
     5041 gggcgctggg gtggtgaagc acgacgacct gcgcgatgaa ctcatctgca acggtcggcg
     5101 gcacgtcggc gggaccgcag acgtcgccac ggcggatgtc ccccttgccg ataccacgga
     5161 cgttgaaccc gacgttgtcg cccggaagcg cctgtgggat ctcctcgtgg tgcatctcaa
     5221 tggacttgat ctcaccctct ttgttggcgg gcatgaagct gaccttcatt cccttcttca
     5281 tcacaccggt ctcgacacgg ccgacaggca cggtcccgat accggagatg gagtagacgt
     5341 cctgtatggg gagacgcatg ggcagggttg tgggcttctc gggttcggtg agcaggttga
     5401 gtgcagcgag tagcgtcggt cccttgtacc agggggtgtt tgcactgggc ttggagatgt
     5461 tgtcgccgac gaatgcgctc atcgggatga agttgatgcc gtcgggcttg tagccgacca
     5521 tcttgagcag ctgggagagc tgttccttga cctcgttaaa gcgcttctcg tcgtacttga
     5581 cggcatccat cttgttgatg ccgatgatca gctggttgat gccgagcgta cgggacagga
     5641 agacatgttc cttggtctgc tccatcacgc cgtcaggtgc ggcgacgacc agcagtgcgg
     5701 cgtctgcctg ggatgcgccc gtgatcatgt tcttgacgaa gtcacggtgc ccggggcagt
     5761 ccacgacggt gaagtagaac ttgtcagtgt cgaaccgctt gtgggcgata tcgatggtga
     5821 taccacgttc acgctcttcc ttgagactgt ccattaccca ggcgaactcg aacgagccct
     5881 tacctttgga ttcggcctcc ttcctgtacc cctcgatgat gtggggcgct acggctccgg
     5941 tctcaaagag caaccgaccg acggaagtag acttcccgtg gtcgatgtgt ccgattactg
     6001 ctaaattcat gtggggcttt tcagctgcca tagatagtat cctccactca aataggcctg
     6061 tcagttcgaa cagcctgctt atgcgagtaa cttatacgta tgacgtcctc catataaagc
     6121 atttctatgc gcgccgatag cgtgcatttc cccaaaaccc cgggtaatcg acagaatctt
     6181 attctccgga aatctacgtc ttcgtaccat gaagacactt gagtttcacc gtgacgatgc
     6241 aaaggaccgg gtgacggttg catgcgcaga ccgtgaggtg tcagtccact cccactgcag
     6301 ttactgccgg cactgtgcgg gtgtccgggt gggcaggcgg acgatcccga caccgcaacg
     6361 ccaggctctc tccggcgtga agcagggcgc aaacgcggat gaaaatctgc tcaacgccgc
     6421 cctcatgttc aacaccctca tccgggacgg caccgcaatc gagtgcgagg acgataaggg
     6481 cgaagggttc tgctcgatgt accgccgcta ggtggcggtg cctattctct ataggagcag
     6541 tcaccggccc gtggctgctg gcggccgcgc ctccatccgc tcagcggggt gatctcgcat
     6601 cgccgcctct ccccgcgtgg ggtcggggga gcgcggctcg gcagcgagcc ggtgcgttcg
     6661 gccgttagga gataccgatg gcagcgttca gcaagcaatc tttagcacgg ggggctgccg
     6721 cctgtaccac gtgaccggat tccccgaatc gctgtctatc ccccagcaag gaaaaaaggg
     6781 agtgtgcgtt ccgttcaaac caggttgaac gcgcgcccgg tcacctcata tggccggatg
     6841 gtgacgtcgt ccgccgtccg gaatgtggcg ttcccgacga ccttcacctc atacgtcccg
     6901 atcggcatat accggaaggt gtggggggta acctcgccgg tgttcttgtt gtagaggtag
     6961 atctgtgcgc cggcaggctc gctggtcacg ttcaggctgc cgtaggggta ggcatcaagc
     7021 cggaacgtga ggtgtttatc ggtctcggtg ttcgggtcat cggtgagcag gatctcctgc
     7081 tcggccggca ggtaacccgg acgggtgacg gtgacgacgt gcggcccgtc cgagatgttc
     7141 ctgatgaggt agggcgtgca gaggccggtc ctgaagccgt ctaccaggat ctcggcgccc
     7201 gccgggctcg actccacgag caggccgcag caggtggcgt ccccgggcgt gatcgtgacc
     7261 gtttcccctg tctcggtcac ggtgatccgg tgggagaggt agcggtcgcc gtcattgacc
     7321 gtgatgaacg agttcccgcc ggctacttcg atcgtccggg gcagcctgaa gaccggcccc
     7381 ttaccgttca cggtccaccc tttgcccacg taatcatccg atgagagaga taccttccgg
     7441 acgatggtct gctcctgcgc ttctatcagg acaggagtga cgcagtctct ctccacccat
     7501 accgtcttcg tcggggtgtt gaacgactgg ccctccttga ctacctgcac cgtatggctc
     7561 ccttctctga tacccaggac aacacggggt gtcgtggagg tcatcttttt cccgtcgatg
     7621 atgattgtgg ccccggtggg ctgggaagtg acgtagatgc cgccggtcct gtcctgcccc
     7681 ggttccatct gcggttcgtc gagtttcagt ctactgagac cggcgtagcc gccggcaggg
     7741 gtaaggtctc cgaacagtgt cgtatcgtcc gggaccatga cggttgcttc gtatggcatg
     7801 aactgctcgc attccagacg gatggtgtgg tttccgggct ctgcagggct gatgatggcc
     7861 ggggtgacct tcccgatgta cgcgccgtcg aggtggatcc gcgcgccggc agggtttgaa
     7921 ctgaccgtga gtgtcgccgg caacggtatg gccaccccgt agatcccgaa tctctcaggg
     7981 tatgccgtca ccgttccgtt tgtgaggtcg atcgtggtgg cgacctgctg ccaggcagct
     8041 ccattggcat cgtagcggta ccatgccgca tcgcgggctg cctccccggg gtgtagtgtg
     8101 acggagaacg ggatctgtgc cgtcgcgtct ttcggggcga taccgtagat ccgatccatc
     8161 gctgtccccg aaaccgggag caggtcctgc accgccctga tcgtcaccga tgtgactgta
     8221 gcacccgtgc cgtcggttac cctggcacct ttcgggaata tgagcgatag atccccgttc
     8281 ccggtgcggt agcgcagttc ttcctccagc acgcctgtag cgttgtgggg ca
//
LOCUS       DAJB01000118           10679 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_45731, whole genome shotgun sequence.
ACCESSION   DAJB01000118 DAJB01000000
VERSION     DAJB01000118.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_45731"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1916 a         3401 c         3323 g         2037 t
ORIGIN      
        1 gatcatctgc tggagtgcaa cggtgaagct ggcccgggtg acatccaggt cgacatactg
       61 gatctgggtg ggctccgtcc cttccgggcg gcagatcata ctcacctggt tccccacctg
      121 gactatccgg atcgggcgtg cgccatcccc ggctccccat accacaccct cacgttctgc
      181 ggcgtcgatg aagacctgcc gccacgccgc cgcggttgcc tcatcgtgcg catccaccgt
      241 caccgtgata tcctctccgg tagtggtggt gacctttgtc actccctccc ggagccgtgt
      301 gtccacccgg gcaaccccct gtcccccggt ctcctggccg ccgaagatct ggacggcaac
      361 gatctccacc cgcaaccggt actccgggct cgcttcagtg ccggtgttga cgttatacac
      421 cgacaccagg ggggagagcc ggacggctgt tcctgagtcc tgttcgagga agaggcctcc
      481 catctggtag acgtaacgct gctgtatcca gtagttgttc gcggaccggt aggcgacctc
      541 acccattggg tccagcacga tcgactcgtt catacccgtt ccagcgattg tcaggttgtc
      601 acggaactcg ttcaccgaga cggtcccgcc cgatgcgaca ggggcaagca ccgggaatag
      661 gacgccgccc ccctgcgtac tgcctccgat cgtcccgagg tcgaacgagg tgctgagcgt
      721 cacgccctct gcggaccgcg tggtattgat cgaactgacc cacagcgaat ccagagcaat
      781 cttataatcc acgaaccggt ccttgaccgc gttcatgtgg gcgatctcgt cctctcttcc
      841 cgccgccggg accccgtaca cctggtagag cgagagtgcg acgacgatca agccgaggat
      901 caggacgaag ccgacgacct ccgagaggcc gtcatcacgc ggtcgggacg gcatagtcaa
      961 attaattctc tgtgtgaggc ctatatctac ctattgttca tctccttgca cgttctgccc
     1021 atgaaacaag aaacagcgga gaatagggcg ctttaacgcc agacccgggt ctctcaagag
     1081 tcccgctcct tcaccgtcaa accttttcac ctcagccggc ccataaatgt tacagtatgc
     1141 cacaggagtc gggagtatcc cccggcggac ggttcctctc tccgggatgc gtcctctgcc
     1201 accagggagc aaagatggtc ctcttcgtga ccggccggtg tcaccgcacc tgctggtact
     1261 gcccgctctc gtatgagcgg aaaggccggg acgtagtgtt tgcaaatgaa cggaaagtct
     1321 cgtcgccgtc cgaggccatc gaagtggcgg agagcatgag cgctctgggg accggggtca
     1381 ccggcggcga accgctgatc gaactcgacc gggtggtgga atactgcagg gctctcaagg
     1441 agcacttcgg cccggaccac cagatccacc tctacaccgg ccttgcgccg aacgaaccca
     1501 tgctccgccg cctccagggg ctggtcgacg agatccggct gcacccgcct catgagtcgt
     1561 ggccatgcat cctcgagacc gacttcgccc gctctgccgt cttcgcccgt aacatgggct
     1621 ttctgatcgg catcgaggtg ccggccctcc cgggaatcga ggaccttgcc gctgccctcc
     1681 cgcttctcga cttcctcaat atcaacgaac tggagtgggg ggagacctgc gccgccgcca
     1741 tgcgcgaacg cgggctcgaa cccgaggacg ggctgcataa tgccgtgttg ggcgcccgcc
     1801 agtgggcgga gaaactctct ctcgaccaaa aagtgcactt ctgctcgtcg gtcttcaagg
     1861 actcggtcca gctacgggaa cgcttaaagc ggatcgctgc aaacactgcg cgtccgtttg
     1921 aagaggtgac ggatgacggg acggttgtgt acggggtgct tgaggcgaca ggtcccatcg
     1981 acgatctcct ggagagcctc gatgaggacg attacgtggt ctgcgaagga agggtcgaga
     2041 tggcgtggtg ggtgcttacc gatcacggtg ccgggctgcc gggcaagaaa tacgtcgtcg
     2101 agcgctaccc caacggtggc atggtggtcg aggtgacgcc gctctgatgc tgcgatcgag
     2161 gcttgagccc ctctacgagc gttacctgca gtggcaggtg aagcacatcc cccggcacgt
     2221 tgcggtgatc caggacggaa accgccggtt tgcccgggag caggggcttg atacggcact
     2281 cgggcaccgg ctcggggcgg atgccacgga gcaggtcctc gactgggcat gcgaactcgg
     2341 tgtgcggcat atcacgctct acaccttctc caccgagaac ttcaggagaa acaaggacga
     2401 actcgcgtcg ctctttgcgc tctttcagga gaagtttctc gtgatcctga ccgacgagcg
     2461 ggtgcacaaa aatcatattc gggtgcagat gatcggcgac cggtccctcc tccccgacga
     2521 cctcctggag acgatcgaag cggcggagag agcgacgctg cactacacca attactatct
     2581 caacattgcg cttgcctatg gtggccggaa cgagatcgtg catgccgccc ggtcgatcct
     2641 cgacgaggta aaattgggca ctctcgaccc cgggaccatc agcaccacga ccgtcgagag
     2701 ccacctcaac cggggcgcgc ccataccccc cgtcgacctg atcatccgca ccggcaacga
     2761 ctgccggaca tccaacttcc tcccctggct cgcgaacggc cacgagtcgg ccgtctactt
     2821 ctgcgccccc tactggccgg cgttccggag gatcgatctc ctccgggcga tgcgcgtcta
     2881 tgaccagcgc atgcttctca aggagcgtgc aggccggcgt tcctgacggt cactgcccga
     2941 aacggcgtgc ccgggactgg aaatcccgga gtgcccggag aaaatccact tttctgagca
     3001 gagcccagtt gacgtcgaga aagaagagtt cggagtagac cgattgccag atgaggaagt
     3061 cggtcaggtg gtcgccgccg gtcttgatga cgaggtcggg ctcgtagttg aaggtgaggt
     3121 acgactccag caggtcttcg tcgaccagcg ccgggtcgac gccgtcttcg gccatccttc
     3181 tcacgcagcc ggcgatctct tcccgcccgc tcttcccgat agccaccgtc acgtccatac
     3241 cttccccgct gacctctttt tcgtcccggt agtgcagggt caggcgtgcg atcgacgaga
     3301 tcgtgcggat cagcggaagg caccgttcca gccgggccgg atcggcggtg ctgatatgga
     3361 acatggcact ggcgatcccg aggtcgcggc accactgggc ggcaaggtag aggttgcccg
     3421 gggcgtcgag catatcctgc tcggtgatca tgaaacagat gtgttccggg agggtgcgga
     3481 ggtcccggag gaggatcttc tcgtagaggc ggtagatcac gaccgccact ccagcagccc
     3541 ggatagggag agggtattta atatatcgtc cggggtgcac cagccccggc gtgccagcat
     3601 gataccgtag cgcatatttg caaattcgcc attcctgtgg gcatcggtcc ctgcagccag
     3661 tttcactcct ttcttcttcg catgccagat ataaatatcc tcaaggtcga gccggtgagg
     3721 cgacgcgttg atctcaaggg cggtccccgt caccgcagcg tggtcgatca cccggagaag
     3781 gtcgattgcg tacggcggtc tgcggccgag aaggcgcccg gtgggatggc cgatgatatc
     3841 gacgtgctcg ttctccatcg cggtgaggac gcgccgggtc aggagatcct ggtcctgggc
     3901 gaatcccgaa tggaccgatg cgattaccag gtcgaggtcg gcgagaacct tgttcgggta
     3961 tccgagcgtg ccgtcgctct tgatatccac ttcgctcccg gcgaggagct ggcaggcgtg
     4021 ccggcggttg acccgctcga tctcggtctg ctgttctttg agggcatccg gggtcacccg
     4081 cgaggagtgg tcggtgacca cgacgtactc gtagtcccgg gcgtctccgg cctccgccac
     4141 gtcctcgatc gactggtggc cgtcgctcca ggttgtgtga gcgtgaaggt ctccccgcac
     4201 gtcgcgaagg tcgaccaggt cggggagggt gccccggagg gcgagttcga tctcgccccg
     4261 gtcctcgcgc aactccggcg ggacgatcgc catcccgagg aatgaaaaga cctcctcctc
     4321 gctcccgaac gtctgcaggc ggccgcttgc agtctccacg aggccttcgg gttcaagccg
     4381 gtagccccgt tccagcgcca cctcgccgag gcgggccaga aacccccggg agcctgtggc
     4441 gcagaggagc gcggtcccgc accggccggg ctcggtgaac cggatcttca ggtcggcacc
     4501 gccgagagtg agcgagacca cttcggcgct cgcatcggca gccccgaggc tgtttacgag
     4561 ggcgttccgg ttcccggtag cgacgatctc aatcctgccg actgagcttg acccccgccg
     4621 gtaactcccc gcaaccgcat actgcccatc agggaagagc gcggccacgc tcgcgagcac
     4681 ggcgtcggcc tgtgggcggg ccatccggtt cgacctctgg cggaactgcc tgatgcctcg
     4741 ctcgatcgcc tcctccttct ttgccccgaa ccccgggagt gccctgagcc ggtgcccctt
     4801 caccgcctgc tcgagatcct ccacggtcag gatgccgagt ctcttccaga gggtgtgcag
     4861 ggtctttggg cccacgccgg caacgccgag aagctcgacg atcgacccgg ggatggttgc
     4921 ctgcagacta tcgagttccc caaacgaccc ggtagcggcg atctcgtgga tctgcccggc
     4981 tatccggaca cctatgcccg ggatacgggt gagttcctcc tcagtgagtt ttgcaaccgg
     5041 gaaagagagc cgctcgacct gccgcgccgc ccgctcgtaa gccccggtcc ggtaccggtc
     5101 atctcccgcg atctcgagca gacggcccat gaatgcgagc cgttcggcaa catccctgtt
     5161 cgtgacctgc cgctccgggg tcgtccccat gatcatcgat cgtccgtgaa accatatagg
     5221 cgttgatgct cgccgggtgc aggcgcacga agggcgtcgc cggacgggaa catatgcgga
     5281 cggcacattc cctacgtcac gtcttccgga aagagtgcct ttcctttctt ggtgtattcg
     5341 ataacaccca gttgtttgag gagatcccgg tcctcatcga cttcccatcc cctggaggag
     5401 tggcgctgct ctcatccctc cccttgcgca aggggtcttc ccgcttatgc tgcctacacc
     5461 cccaaatata aacttatagg agtcaaagaa aatgtagcat gcttccttcc acatttgagg
     5521 attaccttga agcggtcctc acgatcacgg gaaacggcgg gcagtcggcg acgctgcagg
     5581 agatagcggg agccctcggc accgaaaggg aagccaccgg gactaccatc gcctccctgg
     5641 tcgaggaagg atacctggag cgggcaaccg acgacgccgt cagactgacc gagaagggat
     5701 tatcggtggc gtcagtggtg gcacggaaac accgtgtcct ccagtgtttc ttaacggaga
     5761 tgctcggtgt cgatgcggac gccgcgagca aggaggcctg cacgctcgag cacgacatct
     5821 ccgacgagac gatcgaccgg ctctcttcct acatggacgg cgcccaaccc ccaacggggc
     5881 gtatgggaca ctgccggggc cgggacgcct gcacgctgct tgactgcaag gaaggggata
     5941 ccgttaaggt ggcgacgata cgggggcccc ggcggcaccg gaggctgctc gacctcggca
     6001 tcttccccgg tgaggtcgtg cagatccggc ggaaactccc gaaccagtcg gtcgtggtca
     6061 gggtgaaagg gtgcgacatc gctatcagcc ccgagatcgc cggatcgatc ctggtggagt
     6121 cgtgtccatg agatttgcgc tgatcggcaa ccccagcgta ggcaaatccc tgatcttcaa
     6181 ccagctcacc ggcctcgggg tcgaggtgag caactatccc gggaccacgg tcgagctgca
     6241 gcggggcaac acctgttttc agcgcgagat ggtcgaactt gtggatcttc ccggcgtcta
     6301 ttcgctggaa gggggttcgg acgaggaaga tctggtccgc cggttcctgg agcagcagga
     6361 caccgatgcg gtcatcgtgg tggtgaacgt cacacgcctg gagcgcaacc tctaccttct
     6421 cctccaggtg gcggagtacg gcctcccgat ggtcgtcgtg ctcaacatgg ccgatgaagc
     6481 cgcaaaattg gggattgaga tcgatcccgc cccgatccgc gaccttctcg gtgtcgatgt
     6541 catccagacg gcggcgtcgc aggggaagaa catcgaccgg atcatcccgg cggcccttgc
     6601 cgccacccgg ccgtcgaaag tggagatccc gtacgaccat catgtggaag ccgccgtccg
     6661 gagtctcggg aagatgtttg gtgtcgaccg caaggagagc gtccgggcgc tccaggggtt
     6721 cggcgataac ccggaactgc tcgaggcggc acgaacgatc gccgacgaga tcgagtcccg
     6781 gcaccggatg acggtcaccc aggtcatcgc ggccaaccgg cacaacttcg ctcaccggat
     6841 cgccgacctc accctcaagg aagagttgct cctccgccag accgaccctg acagtatcct
     6901 gacccggctg ttccccggaa tgccgatcct cctcggcatc ctcgtcggga tgctcctcct
     6961 ggtcttcgtc gtgggatcgt tccttgagga gatcatcgtg gagatcttca acgtatttgc
     7021 catacaaccg ttcctcgcgc tcgggctgcc tccactcgcc gaagaactgg gaagatccat
     7081 cctgctcgcg ctccaggcgg gcctcgggat agcattcccg tacgtcctgc tcttctacat
     7141 catcatctcc gtcctcgaag actccggata catgacccgg gcggccttcc tcgccgacaa
     7201 cgcgatgcac aggctcggga tgcacggcgg ggcggtcatc cccctcaccc tggcgttcgg
     7261 gtgcaacgtg ccggctatca tgagcatccg gctcctgcac tcacggcggg agcgcattat
     7321 tgcctcgttc ctggtcacga tggttccctg ctcggcccgg accgtcatca tcgccgggat
     7381 tgtggggagc ttcgtcggtc tcggggcggc attcagcgta tatggcatcg tcttcgtcct
     7441 gatcctcatc acggggctcg tcctctcccg cgtgacgccg ggcgagcggt tcggcatgat
     7501 catggagatg gtgccgctcc gccggccgga ctcgaaactg gtgatgaaga aggcctgggc
     7561 gcgcctctcg gagttcctct tcatcgcgat gccgctcctc ctcgtcggga gcgtcgtcct
     7621 cgggctgctc gagttcttcg gtatcatggc aatctttgag gggatcgtgg agccctacac
     7681 catggccctc ctcggccttc ccggctactc ggcgacggcg ctcgtcttcg ggatcctccg
     7741 gaaagagatg gcgtttgaga ccctcgccat cctcgcaggc accgccgacc tgggggcggt
     7801 gctctcatcc ctccagctgt acatctttgc ggtcgtcacc gtcctcttcg tcccctgcct
     7861 cgcgaccatc acggtcctcc tgcgtgaagt cggttcaagg ataacgctcg cgatcaccgt
     7921 ctacaccgtt gccctcggac tccttatcgg aggccttatg tatcgtatcc tatcataaat
     7981 ggtgtactga gatgtacctc tacgaaaccg gcattccgat gatcgacagg gagtatgggg
     8041 gcctgcgggc tgccgcgaac gtcctcatcc tggcgccgcc cctgacctat gcggagcatc
     8101 tggcatacag agtagcctgc ccccgggcgg gggaatggac ggttgcgatc tcgaccgacg
     8161 agcgtgccgc cgacgtcgtc ggcgcattca ggaggcaggg ggcagggaga caccagatcg
     8221 ggatcatcga tgcggtcacg aagagttcgg tgccgaccct gcgggacacg acgaaggcga
     8281 agtttgtcac gagccccctc gacctgacca gtatgggcat caagttctca aggatggtag
     8341 aggatatgtg gaaggagggg gtgatggccg atcctccagg gccgatgcct ccgccgatcc
     8401 ggctctgcat caactcggtc tcgacgcttc ttatgtatgc acggcttgag gtgaccttca
     8461 agttcctcca cgtcatcacg aaccgggtga agaaacttga ggggatcggg atctacgtcc
     8521 tcaacagcga gtcgttcgac gaccggacgg tatccacgat caagcagttg atgaacatgg
     8581 tcatcgaggt gaggacggat gacgggaggc agtcggtgga gcgggacttc cgcgtcatcg
     8641 gcatccacgg caggacgacc ccctggatcc gttacttcta cgatgatgga acactgacag
     8701 ttgagggatg acgatgaaac ccttgcagat gcgtcccggc tgcctcatgc cccggatacc
     8761 ctgccttcct gcgttcagcc tcgcttctgg agcgaaccgc ccatgctgat cttcgtgggt
     8821 ctggggctct acgacctcgg ggatatatcg gtcaaaggcc tcgaatatgt ccgtaacgcc
     8881 gatgccgtct acctggaatc gtacacgtcg cggttgatgg ggacggatgt tcccgcgatg
     8941 gaggcgttct ttggcaagga ggtccgggtg ctcgggcgag aggatgtcga aggggacccc
     9001 cgcgagatcc tcgagcgtgc cgcgataggc cgggtggcgt tcctgaccgg gggagacccc
     9061 atggtctcga cgacgcatgc cgacctgcgg atgcgggcag ccgccgccgg gattgagacg
     9121 tccatcatcc acgcctcctc gatatcgagt gcggtctcgg gcctctccgg gctccagaac
     9181 taccggttcg ggaagtcctg ttcggtgccg ttccccgcga aggggtggtt cccgacggcc
     9241 cccctcgaga cggttgcggc aaacctcgcg ctcaacctgc atacgctnng aagagatggc
     9301 ggagaagcgc ggcatcgagc cgcccggcct gtacgtgggc atcgcccggg cggggtcgga
     9361 gagccccgtg gtcgtggcgg ggaccggtgc gaagctcaga gagacggagt ttggggcccc
     9421 gctccatatc ctcgtcgtgc cggcggacct ccaccccatg gaacgcgagt acctggagat
     9481 ctttgccggc ttatgaccct cgaggagtac ggtgccgtct ttttggaggc actctccctg
     9541 acccggattg ccgtcccgga ggagaccctc cttcaccagg tggccgacga ggtgctcgag
     9601 atggctgcgg cctaccagag cgacggcacg gcgtttctga cggatgatcc ggtgaacgcg
     9661 ctcgccgcct ttgcgtacgg gctcgggtgg cttgacgcag gctcgcggct cgggctcttt
     9721 gagccgctcg ccgcccaccc gccggacggg gtggatgcag ccataccgga ccgatatggc
     9781 gcccacctgg acgagaagac ccaccgctac cggcggatgc ttcgctccgc tcttctcgcg
     9841 gttcgcgcgg gcccggacga ggcgagcccg ctctctgcgg gggcggaggt gttccgctcc
     9901 gcggcgtctt cccggtatgc ggagggcgta gaacgccttg atgcgggcga ccttgccggc
     9961 gccctcgccc ggttcagcta cgggtatgcc tggcttgatg ccggtgtccg tgcgggactc
    10021 ttcaggatca ccggcgagcg gggtctcttc accgtctaag gaccggcgcg gaagcagatc
    10081 cccggcctgg cttgaggtgc aaaatgagcg gctacgttcc tcgacacgct ccagggggag
    10141 aacgctgcct tacggggcga acccgagcag atccagcgac agatcgcgat gcaggccggc
    10201 atcagcgcga gcccggagaa acctatttta ggtacgccgg gatatcatac cctatcagga
    10261 gggaaagatg gcaaaaccga ttgaacttgg gcttgttctg gagggagaag atgcccggcg
    10321 gttccagcgc tatctggacc ggcccacaga caccgatgac ggccgcgagc tgatccgcga
    10381 agccgccatc ctcgcccgcg agatgcgact ttgaacacaa aaataccctt cgataatcta
    10441 tctttttctc tctatcgtga cttctatcgg tcctaccagg aagccgagaa gggtatgagt
    10501 ccgcccctgt ctgttttcga tggcggcggc cgtcgttgac gggttccttg gtaaactgag
    10561 gtcatcccat aaccctccag atcatgatgg cacatgccgg gtggatcaac ccggagaatg
    10621 agtgttcata tacgacgggc aaggagaccc ccggtttcaa ccgggggagg aattgcccg
//
LOCUS       DAJB01000119            4884 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65287, whole genome shotgun sequence.
ACCESSION   DAJB01000119 DAJB01000000
VERSION     DAJB01000119.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65287"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          901 a         1500 c         1407 g         1072 t
ORIGIN      
        1 ccgggttcag tcatggccgg ctacctcctg tcccgtcagg gttgtgacgg tgtagtagtg
       61 gcagccggtc cggacgatgt ggatctcccg caccgcccgg ccgtatcggc cgatctcggc
      121 ggccacgacc tgctggacca ggtagagcgg gacgacacct gccattcgct tttcgggagt
      181 tggacttata actccggacg gagaagagtt gttggcagtc ccgtgtgaat cgatgttggt
      241 gcacgcttca tcccgtgggc tgccgtctat cctgtctgtg cgttgcattt cgttccctcg
      301 ttgtgtggcg ttatcaatct ggctttaccg tcggcgatcc acttctccca gacgagtttt
      361 gcaatggggt ccgggatgtg gtcgagtact tcctgggtga tctcttccat gcggttctcc
      421 tgtgcgaaga tgccgtggga tcggcgttag gccgtcggcc tctctcacac acagaatagg
      481 tgggtgaggg gtgatttaag cgagaaccgc gctcggtgta tgtgcatggg ccaactgcac
      541 aggagaccgg cagagctgct acaacgacgg agcaagcgtg atagaaagca gcatcgcgat
      601 tcagccccag ggagttttac taaaaaaatg gagtggagtc ttattgagcc gggaggccca
      661 ggtctgctct catcttctga atcgccgcgt cgtaatcaaa gctctgctgt gccttctccg
      721 tcttgctctc caccatcctc gccacttctt cgacgagtat catagagcac ttcgcgcaga
      781 acagggcatc ataggtgttc agggccttgc accgggggca ctccagtggc tgaaggctgg
      841 gttctttatt caccgtgacc tccacaaacc cggctttttc gagcatcttc ctttcaatgt
      901 ctccaccgct gaggtgtacg tacacttcag gcatgttact gctcttctcc cacccggcaa
      961 tcagacgaag ttccatctca gagaatccct tctttacgag gtcggttagc ctgccgtgtc
     1021 ggatggcgtg tggattggtg ggtttcccca gattcaatgt cttctggaga tacttgaggc
     1081 ggttctggac ggtgcgtgaa ttcaacctct tgcgaccgac cccgaatcgg gtattggtca
     1141 ggaagagagg ggctgccggg tccgttcgaa gcgggtgcat gttcaaccag agctgaagat
     1201 caggaacgct gtcaatgagg cgcaggcggc ggcggccggt cttcccatcg acgattacca
     1261 cagcgccgta tcggtcgaac tgcacgcggc ccacattgag ggagagtatt tcgctgatcc
     1321 tgcacccgga gtcccacagg agcataatga gtgcccgatc ccgctggttg tcgcaggcac
     1381 ctacaagggt tttgatgtcg tcgcgaacaa tgagctgctc gaccggcagt ttcttgcgtg
     1441 ggcgtacgtt cttgatgttc ttaaacaact ccttctctct ctccggcacg agccagcgga
     1501 agaacaacct ggtctcaagg atctcgccgt atagggtgaa gggactgcac gtccttcgcc
     1561 tcctgatgac aaagtcttcg aggtccatcc gtgtgatggt cacggcatct ttgaatcctt
     1621 tttggaaaga gttaattctc caatccaaca gcaccgtaac cggttcagac ccccgatcct
     1681 cccactggac cagttctgcc ccaccgggca ccttcaacat gcagttcacg ttgtctaccg
     1741 caccctcgcc agccgctttg ccatccgcaa gccatcgtag cagaggtaga ccccccgccc
     1801 cgtttcaacg acgacatcgg tcgccggccg cagacgtggc agggctcgtc ctttgccgcc
     1861 gggagggggt gaggtgaacg acggttcgta gaacgacgtt ccgagggagc gtagttcgag
     1921 aaacccactg ggtttcgagg acaggagttt gagcaccccc aggtgcgagt ggcggggttg
     1981 accgggacag tcgtttgcac atttacaccc gttttcacat ctttacaccc tgatttacac
     2041 cacgaaaagc cgcctgatgg agccggttct cggatcggcc ggattccctc cggaatattc
     2101 gggatgtttt caccacggga agctattgca tacatcaaga agcacccggc gatcctgaac
     2161 acgctcgaca ccccgtatac cgtgacccgg gaagtaatgg aacagtacaa cctcagagtt
     2221 ctcgacacat cagacgtcct ggctgatacg ctcaggatct cgcagcagta tggattattg
     2281 ttctctgatg ccctgcatgc cgcgtcggca tgccggcacc acatcgacta cttcgtgacg
     2341 aatgaccggg attttgaccg ggtagacttc cttacgctgg tgcgcccttc tgctgaagag
     2401 agctcctgac cgcccatacc gcatgcatcc tcttttatat cccgggattg cggtatgtac
     2461 ccgtacacct ttttgcgggt gcgtcggtct ttcctgatac tcaacggctt ttaggccctc
     2521 actgcagaag atgccatatc ctctcatgag gaatgatatc gatcgcctgg agcggcagaa
     2581 gaggagagct ccttgcgtgg acggaagaac cagggggtca gccgtgtcga agtcctcaca
     2641 tcaccccccc cgccgagtac cttcacgatc accgccacta tccctccaac ccactccacc
     2701 tcggagtccc tgtacgccgg ataccgcaac ccccacgccc gcaccaatgc ccggtcgatc
     2761 gcgaaccccc cgtcgtctcc cgcgccgcaa atcacgggcc cggcggccgg ccaggcccga
     2821 tcgttgtgcg tcaccggtgt cgcactaccg tttttgtgcg gcatgaaacc ccacttcatg
     2881 tcgcacaacc gccatcctgt atataaatgg gtatactgca tcggggtttt gacatacgca
     2941 accgggcttg ctgtaccaga acactcacaa ccggtccccc tcggtgatgg cgagcaggcg
     3001 tgagaagaca taggttgtgg cacgccgtcc cccgccttcc tcaaggacgg cgatgatctc
     3061 ctgttcccgg aactgctgga ggatccgctc tgccgccctc cttgacatcc cggtctgctc
     3121 gtaaaactca gccgaactga agaccggcgt tttaaagagc gcgtctatcg cggcgatcgc
     3181 gtactgcgac cgggtcacct ccggcaccgt ccgcttcatc tcgtcataca ggtcgaggat
     3241 cgccgttgct ttccggccgt tcgccccggc ctgctcctcg atggcgtgga ggaagaacgc
     3301 gatccagccg ttccagtccc cgtcccgcga gaccgcgagc agccgctcgt agtagaccgg
     3361 gcggttccgc tcgaggtagg cgctgatgta gaacatcggg ctcccgatca gccctttctc
     3421 gtagaggatg aggggcacga gcatccgccc gatacgcccg ttgccgtcgc agaacgggtg
     3481 gatgagttca aactgagcct tcaggacgga gagctggacg aggacgtcct tctcctcccg
     3541 ctgcaggtag gcctcccagt ccccgagcgc cccgggaaca tcctccggcg ccggcgggat
     3601 aaagatcgcg tgttcgatgt gagcgtcccg cccgatgaag ttctggatcg tgcggacgca
     3661 ccccggctcc cggtccatgc cccggctgcc ggcgagcagg atccggtgca ggtcgcagac
     3721 gagggccaga tccagccgtc gggtcttgag ggcctccacc gcggtattca gcgcctcccg
     3781 gtagttgatg atctcctgaa tgtcggcnng acagcaccgc ctcctgcgtc aggagcgggg
     3841 agaggaggag ccgtgggttc gggatcgcct ggaggatgcc gtcgtaccgg gcgagggcgc
     3901 ggttcgccga cgcaatccgg gggatatggg tctcccagtc gatgcctgcc gggggcagcg
     3961 gctccgggac gtagggttgg acggtcatgc tgtctcctca cggaccgtcg tgaacccgtc
     4021 ggaaacagga aaactggcga tatgccggag ctccttcata caattattcg atggtgcgac
     4081 cgggtattaa ccttgattgt ttgcgatccg gacgttcatc atagtccgtg ccaatcccgc
     4141 cggcttgtct gaatatttcg ttcgagaatg tccctggccg gggaggtgcc ggcgtcttca
     4201 ccgtatcgta ctcccggatc aacgatcgtc tcgcaatctc tcaagcgcct tccggaccgc
     4261 atgtttnngc cttcatgaga agcagccgcg ctctggggtc gccgatcttc cccaatgcat
     4321 cggcagcgct cttgcgcacc ccgccgtccg gatcccgcag gcattccgtg aggggataga
     4381 ccgatccttg gtctttgatg accccgagaa tacggattat ccgcaacttc gtcgctctat
     4441 ccgtgcgttt cagctccgcg atgagcgggc cgactgcagg cggcccgata ttcatcaggt
     4501 tctgagcggc gcggtcctgc ttcccctggt ccttcccatc tttgagcgcc gcgacgagtt
     4561 ctgcaacccc tccgtcagcc gtatcgggtg tctgttccgg gatgcctatc ttcggcatgt
     4621 ccagcggtct tgtcggtgga ggtgaggttc gtacgtcctg ctttcccatg agaagcaggg
     4681 atcgcagcgt ctgcgggtct ctcccggaga tgatgagaag ccagcggagg ctcgatacgt
     4741 tgatcgtcga ctcattgccg taggcctgcc ggagcagatg gagccactgc tcatcggtga
     4801 tggcacccga tcgggctcca agcagcgcaa tcaactcttc gtgagcctcg cctcgtatcg
     4861 aacctgaact cccggatcct tagg
//
LOCUS       DAJB01000120            5016 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_100425, whole genome shotgun sequence.
ACCESSION   DAJB01000120 DAJB01000000
VERSION     DAJB01000120.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_100425"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    506..518
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1057 a         1467 c         1407 g         1070 t
ORIGIN      
        1 agagcggatg cacgaggcga ttgagttcca catcgagggg ctcagggagg acggactccc
       61 gattccgccg tcacgatctt cagccatcta cgtagcggtc ggcagaggat aactcgcccc
      121 ggtcgggcgt tcatccccgg acttcaacgc tgacggtata ttccagcact ccttccccgg
      181 tgggaatctc ctcgcccttt tcctggagac cttcaatgta gaccnncggt atacccgcct
      241 tccggctcct tttggaggag gaggcggtac tgcaggtttc ccatcttgcg cttgccggcg
      301 gtgctcatgg catgtcttct ccggaaccgg gtttcgccag cgcagcccgg cccttctcag
      361 tcaggcggta cttctgatac cgactaccgg gtttctcagg gatggtgtat tcgatcatca
      421 ggtccattag taattgacgg acgatcttgt tcagctgacc agagatctct ttctgcccga
      481 gtccccttga caactccgct ttggannnnn nnnnnnnngt gactctggct gtgactctgg
      541 ctgagccccc tggccgggag ccagagtccg gcggatgatc gtctggaacc catcactgac
      601 ggcaaactcc ggctccggca gaccggcctc tatgcagcgt tggatcatgt cccgcgttcc
      661 cgtgcccatc cgctcgatgt agcctgtcag gtacatcggt tccgccagga gcgggttgcc
      721 cggcaccgag ccgtgggcct gccggagttt ttcgagagtc agggacggcg ggagcgtgcc
      781 cgggttccag atttccaggc gatccgcaaa gagcatcacc tggacgctgc cgttgctcgt
      841 gtagtcgcgg tgggcgacgg cgttgacgat cgcctccgcc accacctcct tcgggatctc
      901 atagcgcacc ggggcctgcg ggccggcctc gcgggtgccc accgagaggg cgatcttgct
      961 caagacgaag tccaccgccg catccacgag atcgaagacc gtgcccttgt agacctgata
     1021 tgacgggatg ggctttgcca cctccatgcc gtggaagtgg gcacacttga tctcggagga
     1081 gatcaggaac cgttgcggct gtttcccgaa gaggaggacc gttgcgttcg tcagccgcct
     1141 gtcatccagc aggttcaggt gctccagcag ttcagtgggg gaggcgtccc cggcgagagg
     1201 gaaccggcgg gtcttcctgg ccgtgcggat gaaccacgcc atacgctccg gatccaggtc
     1261 ttttagcgtc gctttcgtgc acggggccgc gtcgaagggg ccggagcgga tgagctgctt
     1321 ctccaccagg taatcgacca gcgccgcata caatccggtg accagttctg agggagtgtt
     1381 gaatcgcttc cggataaggc ctccctgcac cctatcgatg agtgcctgca tccggggatc
     1441 ccgggctgca tcgtatgttc ccctgacata gatcaaccgg tacttgccaa gagcagtggc
     1501 atgatcgaac tcccgttccg tcggggagat gccctcatcg tccctggaac cgtaggtcgt
     1561 accaaagagc ccgacataca gatcgcaccg ttcgacctct tccaggtaca ggtcatcggg
     1621 gggcggtcgg cggcaggcac atcctcaaag aggaagacct cgaagaaccg ccgcatcagt
     1681 gcatcgcccc gtagggaatc acggagcgcc gcccgttccc cggaaaactc tgtttgcacg
     1741 ctgctgatga agaggcggat cggcttcacg tggcacactc cgctgcttct gccccgaccc
     1801 gccgcaccct gccggtcagg agctgttgta ccatgccctg tttgccccgg cacaaaatgg
     1861 tgtgaaggcc agcgatcagg tctctttcca tcgcgccctg aacctctatc tcattttaag
     1921 gtgggataca cagaaaaggg tgttcaaatc gatctcaaac cttcgaggtt atcccaaaac
     1981 gcctctgtcg ggagggggta ccctcgcacc ccctctatca ggttccactc cctcaccagc
     2041 cggacataac accctcgaag cctgacggct tctcatgctc gctgcgctcg cacctcgcac
     2101 cttcggtgct cgaactcctt ttcctccggg gaagtcgttc cttgaagacc tgcggtcttc
     2161 tcatgctcgc ttctctctcg cacttcacac ctgctggtgc actcagtttg atcatgagaa
     2221 caacgtcata attcgggcta attcgttgcc gaaacagaca tatagttctc cgaatatgga
     2281 tgaccatgaa gatcatacta accagtgtat ttgtagacga tcaggacagg gctctgaaat
     2341 tctacaccga agtgctgggc tttgtgaaaa agagcgatgt ttctgctgaa gactacaggt
     2401 ggcttaccgt cgtttctcct gacgatcaaa gcgggaccga acttttgctt gagccgaata
     2461 gcaatccggt agcacaggcg tatcagaaag gaatattcga acagggcatc cctgccgcgt
     2521 cctttggtgt tgaagacacc catgcggaat atgaaaaact aaaagaacag ggggtgaagt
     2581 tcacgatgga gccgacagaa gttgttgacc cggtgaccat agcagtcttc gacgacacct
     2641 gcggcaatct catccagatt caaacaacgc aaaaataagg tattctcatt ggaggcagcc
     2701 atctcccaaa ctggtggaac tcctctattt ttcaccgggt caaccccgtg caacattgta
     2761 ggtatgacaa aaacttacaa tactgtcatg gctgcaacga aaagggtccc cgtctcggag
     2821 gaagtatggg cggagatctc cgggctgaag tgccccggtc agacgttcga tgacctcctc
     2881 tcgtacatgg tggagcagga gaagaagcgc cggttcatcg aggatatgga cagcatcgag
     2941 gccgaagggg attttgtggg gctggacgtt gacgttccgg atactggcta actgcaaggc
     3001 gcttgagagt atccggagcc ccggcgagaa gagccggcga tgcagcttcg cttccggata
     3061 cgccgttctg agggttttcc tggcatattt tcttctccgc ttctttcgtt tcgagtgtac
     3121 gcacactgtt gcaggtgtat gtagaccaat cacgcatcgt gacacctgta cgacgacatg
     3181 gtgagcggga taccgggccc acacaccccg gggagcaggc cgacccggga cataccacat
     3241 ctgttacata acagatctgt tatacaatac tcctgttagg tgccgtgcat gttgcctgtg
     3301 ggaggcccta caacggaggg cgataccatc gaccgcgttc gtaaaatcgt gttcgttctg
     3361 agcacgaacg aagtagatca cgttatactc accgtcctcg gcatggacat ggtatcccgg
     3421 catgccttca aaagaaggtc ccgcgggtcg ccggtttagt taggttatac cccaacaaaa
     3481 acaaacattt atgctggagt atactctaac aaaaacatat gcccccggtc ctcgacctct
     3541 tgaccgccct caacccctgg tggagtggca aacccttcga agcgggtata cggcgggaga
     3601 agtacttcac gaagatacag aagtatcttg gaaccgggga gatcgtcgtc cttacagggg
     3661 tgcgtcgttc gggcaagacg acgctcctct ttcagatcat cgacgacctg atccgcaacc
     3721 gccgggtcga cccgcggtcg atactctttg taaactgcga tgagccggag attgcccgcc
     3781 ttgtgaatcc gctcgagacc ctgcttgaga cctaccggag ggacgtgtac ggcggtgatg
     3841 tggcctacct tatcctggat gagatccaga ccatcgaggg atgggagcgg tgggtgaagt
     3901 cgctctacga ccgcaagcag ttcacgctca tcatatccgg ctcgacgtcg tacctcctcg
     3961 actcaaacct ctcgacgctc atagccggcc ggtatctccc gatccatgtc tacccgctcg
     4021 actttgcgga atatgtggtc ttttcgggcg aggatctgcc gcacgatccc gtcgcgctcg
     4081 cctcggcgaa gtaccggctc ctgcacctgc tcggcgagta tctcagggaa ggaggctttc
     4141 cgcaggtcgt cctccaggac gatgaggcga tccggcggga ccagctcgcg gcatactacg
     4201 acagcatcgt ctatcgcgac atcgtgcggg tcaacgaggt gcgcaaccag aaagcgctcg
     4261 gcgatctgct gacttactgc atgacgaacg taacctcgct ctactcctac cggaacctcc
     4321 aggaggtgct cggcattgat ttcgagacca tcaaggagta tctctactac gcacagaagg
     4381 cgaagatcct ctttgagatt cagtacttca gttactcatt gaaaacgcag agcaggaaca
     4441 acaaaaagat ctactgcatc gacaacggcc tccggaacgc tgtatcgttc cggttctcgg
     4501 aagacgaggg aaggcttgcg gagaacctcg tcttcatcga actcaagaag cagggccacg
     4561 agatctacta ctggaagaag cagggcgaag tcgatttcgt cgtcaaacat cccgaccggt
     4621 cgctctcggc aatcaacgtc tcgtatacgg atgccctccc cgcccgggaa gaaaaagcgc
     4681 tgcttgaatt cgcagacctt cacggctccg gggtgcggga gtgcatactt ctcacgaaag
     4741 acacggagaa gagggaaggg acggtccggt atgtcccgct ctggaagtgg ctgcttgggg
     4801 ctggttgacc gggcaggggg cggcaccctc ccggggattt tgcaggggtt gatccgggtc
     4861 cccaccaaca gactgtggta tttcaggatc gtttccggca gtccgggtct ctcgccccag
     4921 caccggcgcc gggggctttg tgcgtcttct gccacacttc agtatattaa ggacataact
     4981 taaagttaca acgcaccatc cgcagtaaga aactcc
//
LOCUS       DAJB01000121            3370 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_128456, whole genome shotgun sequence.
ACCESSION   DAJB01000121 DAJB01000000
VERSION     DAJB01000121.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_128456"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          718 a         1042 c          976 g          634 t
ORIGIN      
        1 ctcctcatca acatcaccct gccggcgctg atcgtcgcct cgatgcaggt gcccttcacc
       61 cccgaacgcc tcgcgggtgc cgagaccctg atcctcgcga cggcggcgct ctacgtcata
      121 tcgctcgcca tcgcctgggt ggtctccaaa gccatgcgtg tcccggccgc ggaagagggg
      181 gtcctccagt tcgcgatcgt cttcggcaac gtcgggttca tggggttccc ggtcgccctg
      241 acgctcttcg gggaggactc cctcttctac gtcgccatct tcaacctctt cttcaacgtc
      301 ctggtcttct cggtcgggat cgcgatgctg accgaaggga gggggaaggg gttcgacccg
      361 cggctgcttg caaaccccgg tatcgcgacc tccgtcgtcg ggctcgccct cttcctcggc
      421 tcggtggaga tcccgtcgcc gttcatcgac tcgatcgacc tcctcggcgg ggtgacgacg
      481 ccgcttgcga tgatcatcgt cggggcgatg ctcgcgacgt tcccggcccg ggagatggtc
      541 gggaactggc ggatctgggt cgcaagcgcc gtcctcctcc tcgcggtccc ggtggcctac
      601 tgctacctct tcgccccggt ctttgccgac ccctacatca acgggatcat gatcacgatg
      661 gccgcgatgc ccgctatggc aaacaccgtc atcttcgcgg agcagtacgg tgccgattcc
      721 cggcttgcgt cccagatcgt cttcgtctca acgatcgggt cgctcgtcac catcccgctg
      781 atcacgacgg tgctgctgta ggatggggac aaactcccgg ggggtcgacg acccgttcgg
      841 cgggaacctc gtccccggtg cccggtaggt gcagcctgca ttttctgtac ggagttcttc
      901 ttttcgcctc gatccgtcac cccggaagaa aaggaatata tttcaacgtg taaaaatata
      961 gagtgacata caaatggccc gggaggatca tatggaatgc atgaagtata taggtctgct
     1021 cgccttcatc ctgctcattg caggcgtcgc cacgtctgct tctgcgttcc acatagagag
     1081 agaacagatc tctgcggccc cggattcgct cacgacacgg gaaaacgtga ccatatccga
     1141 gctcatccga ttcgagtacc tgaacggctc gacattcccg gaaaaccatt tcgttcgctg
     1201 ccggagcgat ctggacgaag tgcggtggac gtatgaagtc atggtcgacg gcgatctccg
     1261 gctcagggag acaaacaagg acccttcctt cagcatctcc ggtttcgtgc tctcgtacca
     1321 cgagcatgcc ggacagtcga tgaaggtcaa tcttaccgta gaggggaggg tgccggaaaa
     1381 accggtcggc acggagatca cgctgctcaa ggtctggcag gtggacccga cgggcgcccc
     1441 cctccccgaa agccactata cctacctgaa agaaggcagg attacaaaaa aattcatgcc
     1501 gccgacaccg gagcccacgc ccactcctgc ccctacgatc ctctccatat cgtcgatgcc
     1561 ggaaggggcc ctcgtatttg tggacgatag ggtggagggg accaccccgt gcaccatcag
     1621 ggacatcggg gcaggggagc ataccgtctc cgtcaatctc accggccacc acatatggtc
     1681 gtcgagcatc gacgtggaag gaagcagaac aaccaggatt tcggccattc ttcagcaggt
     1741 cccgaaagaa gaggggattc cggtcatcgg cggagtcatc gagacggtcg ccggcgtctt
     1801 tagcggcggt gaagatgaga gggccgggcc ggaaggcgag gagggaaaag gggatgaagg
     1861 catcccggtc atcggaggcg tcatcgaatt catcaccggg ttcttctccg gggaggatga
     1921 acctgcagag ggaggtatgg acgagaggct tgcaaggctt gcggagctcg aaatcgaaga
     1981 cgattcggat tgatgcctga cactctccac ggctaaagcc gggggcttgc ttgcgccccg
     2041 tgaaccccca ttattctgtc aatcaccgca tccgaagaac ggcccgggac caggagcgcc
     2101 ccagaatccg ggactccgcg cggcacttca cagccgggcc atcccgcccc gccgccttca
     2161 cccgccgacg gaaaaacggg agaatatata taatactata gtgtatttag gaaatcccta
     2221 tatgtgcgga actcccataa tctgatatgg ctgcggttcg cctgcgcgaa aacgaagacg
     2281 gggtgtcggt catcgtcggc acgctcatgc tcatcctgat caccgtcact gcagctgccg
     2341 gcctcgccat catgatctcg gagtttcaaa aggaggatat ggagcggcag agccatatcc
     2401 ggggcgtgga gcacgaaaac ctgagcctga cgcacatcga tcttacgaac aacaaaacgt
     2461 tctggatcga gtggaacgaa atagactatt ccggaaactg gagcacgctc gacctcacca
     2521 tcctgaacct aaacatcgac gactcctatg tgaccgccat cagcgtgaac ggcaggtacg
     2581 caaagaacta cacctataac gatgtggagt acagcccgac caaacggctc ctgatccccg
     2641 ccagaaaaag cagggagaac gtgcagatca acttcacctc aagttttgaa gaaccgttct
     2701 tcgtcggcac cggcgaggcc cttgacgtcc tggtgatgac gtcgctttac aacaccttcg
     2761 agcggacatt cgagcagccc acgccgatcg tgcacttcac catcagaacc gaggatctgg
     2821 gattcgtcga tcgcgacctc ctggtgctcg acgggtccga ctccttcgac gacggcgccg
     2881 tcacggcgtg gaactggacg atcgacgacg gatcgaagac ggtgcccgcc gggaactggg
     2941 cggactcggc aaatattaca ccatcgttcg cggaaggccg gaaggcaacg gccaggctcg
     3001 actcatcggg gcctctgcgg atcaatctca ccgtgaccga cgatgcgggc atgacctcat
     3061 cgtcgcagta cacctatatc cccgccaacc cgatgttcaa cccggcgacc aacctgcagg
     3121 tgacggtcgg cggcaacctg gtgaatgcaa cggtgctcga catcggcggg aagccggtga
     3181 agggcgttgc cgtcaacttc gtcaagacct acgaccccta tggaaacctc accatggaga
     3241 actggtcgca ggcgaccgat gcaaacggtg ttgcactctc gacgaaacag gacggaacgg
     3301 gaacgatacg cgtcatcgcg gcgaagatcc cgtccgtgga cgtgccgctc taaaggtcga
     3361 aatcgccccg
//
LOCUS       DAJB01000122            3458 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_35804, whole genome shotgun sequence.
ACCESSION   DAJB01000122 DAJB01000000
VERSION     DAJB01000122.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_35804"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          610 a         1049 c         1151 g          648 t
ORIGIN      
        1 accctccccc tgatcgggtt ctacggaaaa ctcgggtttg aacccatccc cgaggcgaga
       61 ctgccgacaa gcatcaggga gcggttcatc ttctgcttcg gggagatggc ggggtgcaat
      121 gtcgccccga tgatgcggag cccgggggat cgggcacggt aacaaaagac aggttcagtg
      181 ctcgatcttc ttgatgtgct cgatggcgtt ctccgccagt ctctcccgcc cctgcagttc
      241 ggcgacatcc cgtatcgcct cgagcatgtg gacggagtct tcgagaaaac tcaggatatc
      301 ggcggggtag aggtcgagcc cgtattcgtc gaggaggtgg gcgctgatct ggcggtgatc
      361 gagccccatc tcccgaaact cgatgatggt taaggtgaac ttgcgctccg ggcacccgca
      421 gaagggggcg ttcttgcact tgcagtccat gaagtcgcgg aaaaaagcaa ggagctgctc
      481 atgcatccgg cgatcgaggt tctcgaagtt gatcgccttc ccgagagatt cgagaaacgc
      541 gcccgagaag acatggtccg ggagccggaa ccctgcagct cgctccagct tccgtgccgc
      601 ccggaaccgg gccttattcg caatcacgac gtttctccgg gctcctcgtc gaactttgca
      661 agcgacttca tagcgttgct catggtctcg atctcgatga gccactcgtc gaagaggccg
      721 gggttctcgg catcgtcctt gatgtacttc gcacagcagg tggcgccgtc gaccacggcg
      781 gccccgatat tcgccgcaac ctcgagcgcc tcctcgaggt tctcctcgtc gataccccgc
      841 cctttcttca cgagtgtctt gatgtccttc tcgaactcgc cctcgaggta cttccggcag
      901 gcggtgaaga gcaccagcag cgagagctgg atcgacccga tgatatcctc gagttctcca
      961 tcgggaagcc cggtcatgac gatcagctcc acgtcgtcga gcttcgcggt ggcctcatcc
     1021 ttcgtgaacc ggccgttctg gtagaggcgg atgatcttca agaccgagag ggtgatgtcc
     1081 tcggtgaaac cgtagagcat ctgctcgcct tcggagacct cttcgccctc cgcgggctcg
     1141 aagttagcgc cctccagtgt tttgatccag ttgtcccagc gctcctgatt gtaaaaaata
     1201 tagaagagtt tcatgggttc tgcctgtttg gcactcgctt tcttcgccat atcagtacac
     1261 attcggagtg cctctgttaa aaatcatttg ctcttcgtgt agagagtcga aaatgccgca
     1321 actcgcgaac attcatcagg gaccagcgag cataagatga gcagatggcg agagtttgcg
     1381 ggcttgaagc gctcctgaaa tcttatgagc ccattgcggt cgcgctctcc ggggggactg
     1441 atagttcggt cctgctggcc accgcccgac ggcatgggat ccgggcaatc gggatcagcg
     1501 tcgataccgg ccttaccccg ccgggggaac tcgcggcggc ccgggaactc tcggatcgcc
     1561 tgggtgtccc gcatgtcgtg atgccgctcg atatgcttga gatccccgcg gtccgggaga
     1621 accggccgga ccggtgctat gtctgcaagc gggcgatgat ggaggcggtc gcccgcgagg
     1681 cagagcggcg gggatgccgg acggtggtcg acgggaccca tgccgatgac cgggcagata
     1741 gccggcccgg gatgcgggcg ttgtccgaac tcggtatcag gagcccgttc gccgagtgtg
     1801 gtatggggaa ggaggatatc gaggcccttg cagatgaact cggggtctcc gttcgtccgc
     1861 cgtccgcatg ccttgcgaca cgcatcccac ccggcgatgc ggtcacccgg gagtgtctcg
     1921 cgctcgtcgc ggccgccgaa gccctcctcg cgcaggagat tcccgggacc atacgggtcc
     1981 ggtgcaccgg tgaccgccgg gcctccatcg aggccgaccc ggcacaccac cggaggctgg
     2041 agcggctgct cggtgcagtg aaggaactcg ggttcagcga tgtggcaatc gccccggaag
     2101 ggtacagaga gggaggtgcg gattcttgga agcagtgatg atcaggtacg gcgagatctt
     2161 tctgaagagc gagccggtga aacgacggtt catatcgata atgaccggga acatcagcct
     2221 cgcgctggag gccgaagggc tctcccaccg catcgagacc ccccggggaa ggatcctgat
     2281 cttcggcgac gagccccggc gcatcgccgc aatcgccgcg aagaccttcg gggtggtgag
     2341 cgccagcgtc tgcacggtga ccacccccga tatcgacggg atcgcagccg cagccgtcga
     2401 gcacgcagag cggcacctcc ggccggggat gtcgtttgcg atccgggcgc ggcgttcggg
     2461 cgtcgagggg ttcaacagcc aggaactcgg ggcggcggtc gggtcggccg tcctcgaccg
     2521 ggtgccggag gccagggtgg acctgacggc gcccgactac gagatcttcg tggaagcccg
     2581 ggagttcggc ggcctcgtct acgatgagaa gatccgcggg ccaggcgggc tcccctacgg
     2641 gacgcagggg gaggtgatgg ccctgctctc ggaggggatc gactcgcccg ttgcgtcgtg
     2701 gctgatgatg cgccggggat gcctgatggt gcatgtccac atgcagagcg ggcggttcgg
     2761 cggggcggac gcagaaaaga acgtcctgca gaaccacgcc cggctctccc gctgggtccc
     2821 ggggcaccct ctcgatcttc ttatggtgga tatggagccg ttctttgagg tgatcacggc
     2881 cctgaaggaa ccccgctacc ggtgcatcct ctgcaagcgg ttcatgctcc gggtggcaag
     2941 catccttgcg ggagagcgcg gcgcctatgc gctcgtcaac ggcgacaacc tggggcaggt
     3001 agcgtcccag acactcgcga acatgacggt gatcgccccc gcggcgtccg ttccggtgct
     3061 ccggccgctc atcgggttcg acaaggaaga ggtcgtcgag cgcgcccgcg ccatcgggac
     3121 gttcgaggcc catccaggga atgtcgggtg cacggtcgct ccccgctacc cttcgacggc
     3181 ggcacccgca gagacgatcg cgcagattga ggaggagatc gacgccgcga gcctcgcggc
     3241 ccgggcggcg gggacggtgc ggcggtgccg cgcaaagaac ggggaggttg aggaggggtg
     3301 gtaaggggac cggttgcaac tccggccctg tcaaacacct taaccccagg gttctcacac
     3361 atcctttgct cgtttatgct cattgcatgg ttttcgaggg gatatcgcca tatgggtggg
     3421 gctgacgggg agggggcgtg ccccctcccc tgtctcca
//
LOCUS       DAJB01000123           29197 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_4004, whole genome shotgun sequence.
ACCESSION   DAJB01000123 DAJB01000000
VERSION     DAJB01000123.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 29197)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29197)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29197
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_4004"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         5602 a         9192 c         9072 g         5329 t
ORIGIN      
        1 gcatggttcc ggcaccgacg acctcgaggg atgcccgggc ggggtcgttg gggtacttcc
       61 ggtttgcctg gacgatatcg gcgaagagac cgaacttgat gccaccgggc tcgttggggc
      121 cacgggcacg ccgggcgggc aggtgggaac ccatctggat gacgcctgcg tgcttcgcgg
      181 cgtaggatag gtcggcgacg gctgcttcac cggcgcacat gcggtacgca gcgatgaagg
      241 acatgccgat ctgcatcgca gaccaacggg aggtcgttcc accgtcgcag gtccgggaga
      301 ccgcggtcgg gatgtggatc gcctggtaca tgctcttgcc gacttctgcc ttgagctcct
      361 cggcctgctt ggcggtgaag agcttctcga tgtccagcag gaactggggc tcgatgtcgt
      421 cagcgagttc ctggtcgccg gtgaagacct tgacgtaaca gtcgtcgacg aggccggggt
      481 gagtctcgac catgtgttcc tggacgacgg ctccgccggg catggcgtgg tttaagatgt
      541 ggaggtactc gttgatcgtc tcgggagtaa cttccttgcc cagacgcttc tgcagggtct
      601 ggtgggcgag gtccatcccg acgatgacag tccgcctgat gtcgtcccac atctgctgca
      661 tcgcagcgtt gttgacgaag tgcaggtcgt cgccctccac gaagacgccg gtgccggaga
      721 cctcgtaggt catcagctgg cgctggccca tcgggatgcc accaaggtgg cagcgctcgg
      781 ggtcatacat ggagacgcca cggtccatct cgacggcacg gctcgccttc atgaactcca
      841 tcttacgggg agactggcgg acaccgttga acttgtagaa ctcggcggtc tcagactcga
      901 cgtcctgccc ctggaacttc tccttgaggg ccttcaggaa cagcttctgg gatctctcaa
      961 tctttgccat tgtaatcact cctctttcgg catgaagccg tatttcgtgc gcagcgagtg
     1021 gatgcgctgg atatattcga cgtactcggt gtcatcgcgg taggaagtcc ctacgagcga
     1081 gtggaagatc gtcgtgtggg ccttgagcca cttctggtcc atgggcttgc cgacggcaac
     1141 cgcacggtcg aggggctcgc cgatctggtt cttgacgtac ttgacgatgc cgtcctcacc
     1201 gaggacgtcc ctctggagca tatcgaacat catgccgtct tcagcgagac ggagcgagtg
     1261 accgtgcacg gtcgcaccgc ggatgccgac gagagcggga tcgaacatct cggtctcgat
     1321 gagttccttg gagtacttct cgaggtcacg ctcacggcac tcgacgatct ggcgaccgga
     1381 gagcgtgccg gggtcgatgc cgcggaagcg gtagcactcg gtataggtcc gctggtaggg
     1441 ctgcgagggg gcgttgaaca tcgagtccgc gaactggatg tagcggacgc ggtcgccggc
     1501 cgttgcaccc tcggtgggct tgacgagctt gcgcatgggg cagttgggct cctgctgctc
     1561 ggcgagcgga gggtgggcgc tcggatatgc cgatccgggt gcgcggtgac cgaggatcaa
     1621 cacgatatcc tcatccgtta ctgaacgaac cttctcaagc ttctggccgg ggttcatctg
     1681 tttacgtcgg ttctcggcga caatcgatgt cccgggaccg tactggggct tgtatgccat
     1741 gttttcattc acctcaaagt gattgctatc ttatccattt cctgtcttga atgagttcca
     1801 gccatatttc cggatattct ggttgccggc tgtcaccgtt ccgtgcggat gagcaccgga
     1861 ccttccccgt tccgggggtc cgcaaggccg atgaacattg tatcggcgca aggcccatac
     1921 ttcgcgtaat ccacgagcgt tggagcggtc ttcatgaacc tgccctcctg gagctggtag
     1981 gagaaccccg tgaagaaacc gtcgcagagc gaccggatct cctcgatgac agaggcgtcc
     2041 tcgacctcca ggatgacctc acccgcagcg atacggagcg cgatctcggc atcgccgacg
     2101 tggatcgctc ttcgatcggg gtgcgggttc ggacttcccc gtgccggccc gtacgggact
     2161 ttctttggca ggccggggcc gtggatcatc atccggcgga tgccggcgat gccggcaagc
     2221 atattgagca ggcgttcggc ggtctcaggt ttgagcatcc tgagcggcac gatcctgacc
     2281 tgcggaaacg tggcgttcat cattcggatc tctgctcgct caggctccgt ttgcgatctg
     2341 gtcgatcggc ttgttgaaga cgtcgatctt accgtaggtg tcggcgaaga tcttcgaggt
     2401 gctctcgggc gagaacatct gggttccggc gtcgagtgct gccgcagcga ccacacacgg
     2461 gatagctacg ccgttggcgt ggcgggtcac gacgtggttg ccgttgaaga tcccggggcc
     2521 gccaccgccg tagatcgagt ggctgaagaa cgagaacccg acagcggtac ccatgacacg
     2581 accgtagtcg gtgctcggga gaccggtctc gtgctcgagc aggtcgttga agtacaggag
     2641 tgtcgcggag accgcctggg cgaaccgtcc ggcaccacag ttgacgatgg tggctgccat
     2701 ggttcccgca gcggcgtagg cgttccagag catggggtcc ttcgtgtcgt agaactggaa
     2761 gtacccgccc ttcttgccgg gggcgatgac cttgtcctcg atggcgcgct cgaccaggct
     2821 ctggacgacc gtaccgatgg ttccggtctg gccgtttgcc ttgacgaggt cgtagaccat
     2881 gttgttggcg ttcaggccct ggtaggcgta ggcgagcagc atggcgcgct cgaacggccc
     2941 gacagcggag cccatctcga agataccggc ggtctcgagg gtggatgcga gtgctgcacc
     3001 ctgcagggcg ttcctgccgg tcatcatgac ggtgtggtta accgggatgt tccggagcgc
     3061 gtagccgata ccctcgttgt tctgggggat ggtcaggatc gacgtgacga gcgcaccttc
     3121 catgtcctgg gtgtgcgggt aaccgcccca gcaggccgcc ttgacggtgg atgcgttgaa
     3181 ggcgtcgatg ttgaactggt cgacgatcgc gaaggtggtc gcggctgcga ccgaggtgat
     3241 cgcggcgtcg taggtggacg cgttgaccag gcgcttcgtc gggaccttta cgagctggag
     3301 ctttccaccg ttgaactcca tgatctcggt gtcgtcgcct tcctcaacct ggatcatctc
     3361 cttgatcctg gcgacgatgg cgtccttgtt ctccatgatg gggagatcca gttctcttcc
     3421 cttgatcttg ccctttccaa gcttgccagc cttgagcgca tcctggatgc ccccgaggtt
     3481 tacgttgatc gttctcttgg tcaggtcgat gatcttacct gtggccgggt taaccagcgg
     3541 gctgattctg tcgagggtga cgccgctctt gagcagcttc ccgtcatcag aatagagatc
     3601 gattgtttcc gtgtatttag ccatagtatt tcatcctttt acccttgttc aatgcattcc
     3661 gttcatgcaa gaggccgaag cagacacccg cttcggtctt tgaaggccgc tactgcacgt
     3721 cccaaaaggg actatacgtg tagaagaaga gttattaata aaatttgcga attaagatgg
     3781 acttttttac ataaagtaat atttagatcg attatattta caaacggata ttttaaaatc
     3841 gagatttgat ggttcaaatg gttgaacgcg atacttaaaa aggattcctc cgaccccttc
     3901 tgccaggagg acactcagag gggcttcgga ggagaacacc tattaaataa taaatcggat
     3961 aaaagtaata gcaataagta aaactacttt tgtcggaagc cgttcaatcg tacctgactc
     4021 cataggttag atccacggat atataatgct acccaaacca ggcaggattc tcccaaatcc
     4081 aaacattcag ccccccattt ccttaatccg cccaattttt gtcgaagtaa aagagatgta
     4141 attatatagt gtcccataac ccggccgttg caccaggatg taccgggtat ggagtcatcg
     4201 caggcccgga gattgggcag ccggcagagg ccccacgctc cggcacagcc ggagagatga
     4261 agagacgacg gacctgccgg ttccccgcgc cccacaaccc ctgccgggag tggaggagcc
     4321 gacacccctg cagatcagca ggttaagcac acaataacca ggctatagcc aggcaagaac
     4381 ccaaaaaatc aaaaaatatt gcactattct aaaaaaaagc caaacagatc agagatgtca
     4441 acaacaaccc cccctaaaac cgcttgatgt aaagttgatc atctgaaata acttacaaat
     4501 ccggaatggt taaatacaaa acattacaag ttatgacaaa gcgatccgcc cggcgatccg
     4561 cccggagagt ttcgttttcc ccaggtgggc agaccccctg cattttgccc ggttttccgg
     4621 cgcattccgg taacccggcc aggaggcctt cgccatgcag gaaggtccgg cctgccgggc
     4681 atggaaagcc ccggtagatc ctctcgcaac gcagaggaga gcccatcaag ggccgtactg
     4741 gaagcaccac accctacggg gccgactcgg cggaacttcg ggagaccggc cctatcaacc
     4801 acattgaggc aaaaccatgg cgaacaggat tacattgaac atgatcaccc agcgctccat
     4861 cgaagaaggc gttgcgatgg aaaagggcaa aacgaccccg gaatactttg aggcctgctc
     4921 gatcatcgag atgcacgacg acgatgtcaa agcgctcggc ctggtgccaa accagcttgt
     4981 acgggtgacg agcgaatgcg gaaacgtggt cgtcaagacc gtcagggggc gccagttgct
     5041 ctatcccggc cttgcgttca tcccccaggg cgtctgggca aaccaggtag ttcctccacg
     5101 gacccaggcg acgggggaac cccagtacag cggcttcccc gtgacggtcg agcccgtcag
     5161 cggagagaga cgcaagagtg cgcttgaact tgttcaggga gccgtcggga tgtggagagg
     5221 tgaccaataa tgccaaagaa agttgagaac gtcggatgcc cgtactgcgg cgtctgctgt
     5281 gacgaccttg tcgtgaccgt atcggacgac ggaaagcaga tcctcgaggt ggagaatgcc
     5341 tgtgccatcg gcaaccagat cttccaccat gcgacaggca gcgagaggat ccggctaccc
     5401 cgcctccgcc agccggacgg cacctacaag gatatttcct acgatgaagc ggttgattac
     5461 gcggcgaaag tgctctacaa cgcgaagaag cccctgatct acggattcgg atcgaccaac
     5521 tgcgagggca tggccgccgc ggccaaggtt gccgagaagg caggagccgt gctcgacaac
     5581 tgtgcgtcca tctgccacgg gagttcgttc ctcgctatat tcgataacgg ctacccgagc
     5641 tgcaccctcg gcgagacgaa gaaccgggca gatgtcatcg tctactgggg cgcaaacccg
     5701 gcccacgccc acccgcggca catgtcccgc tactcgatct tccctcgcgg tttcttcacc
     5761 ggcaagggcc acatgagccg caagatcatc gtcatagacc cccggcacac cgacaccgcc
     5821 caggtggcgg atatctacct gcaggtgcag cagggccacg actacgagct cttcgacgcg
     5881 ttccgcgccg tcgtccgggg ccacgagatc ccggacgttg tcgccgggat caggcgggag
     5941 cagatcctcg aggtcgccaa tatcctgaag aaggcccggt tcggcaccat cttctacggc
     6001 atggggctct gccactccga cggccggaac cacaatgtgg acatcgctat cagcctgacc
     6061 cgcgacttga acgacttcac caagtgggcg atcatggcga tgcggggcca ctacaacatc
     6121 accggaccgg gcgccgtctg gtcatggcag tacgggtacc cctactgtct ggacctgacg
     6181 aaaaaggata tcgcctacat gaaccccggc gagacnnccc acttcccgat cgacgcggtc
     6241 aagcacttga ggaagcaccc ctggatcacc gtcgacccga acatcaacat ggcaagcgag
     6301 atctccgatc ttcacatccc ggtaggaatc gtcggggtcg aggtcccggg catcgtctat
     6361 cggatggaca acgtcccgat ccagtaccgc aaggtcatcg atccgcccga gggcgtcatc
     6421 agcgacgaag agctcttcga gcggatctac cggagactcg ccgaactgga agcagaggag
     6481 gccgtaaagg aacaagcgaa ggccgcaccg gaaggcgtcc gcgcggagga gtgagtcatg
     6541 agcgaactca taatcaggaa cgggttcgtc ttcgaccccc tgcaggggat caagggtgac
     6601 cggatggaca tcgggatcaa ggacggcaag gtcgtcgaga cgagcgccct caacaacccg
     6661 aagacgatcg acgccgctgg catgactgtg atggcgggcg gtgtcgatat ccactcccac
     6721 gtggtcgggc cgaaggtgaa cgtcggccgg ctcttccgtc cggaggacaa actcttcaag
     6781 agcccccaca aaacgtgcag ccgtatggag atgggctcct ccgtcccctc tacgttcaag
     6841 accggctacg actacgcgcg gatggggtac gtcttcgtca acgaggcggc gatgcccccg
     6901 ctccactccc cgcacgtcca tgaagagata cgggataccc cgatcatcga caacgccgcg
     6961 atgccggtct tcgggaacaa ctggttcacc ctcgaatacc tcaagaacaa cgagatcgag
     7021 aacaacgccg catacaccgc atggcttctc cgggccacgc gcgggttcgg catcaaggtc
     7081 gtgaaccccg gcggctccga ggcctgggga tgggggctga actgcgtgca tattcacgac
     7141 ccggtcccgt acttcgacat cgccccggca gagatcatca agggcctcat cgagacaaac
     7201 gagtatctcg gtctccccca ctcgatgcac ctgcactgca acaacctcgg gaacccgggc
     7261 aactacaccg acacgctcga ctcactcagg ctcgccgagg gctacgcccc gaagaacgac
     7321 ttcggccgga aacaggtgat gcatgtcacg cacctccagt tccacgccta cggcggcgat
     7381 tcctggggga acttcgagtc ccggtcgaaa gacgtcatgg actacgtgaa cgcccacgac
     7441 aacatcaccg tcgatatggg ccaggtgacc ctcgacgaga ccacgaccat gactgccgac
     7501 gggccgttcg agcatcacct gcgctccctc aacaacctga agtgggcgaa cgtggacgtc
     7561 gaactcgaga ccgccgcggg cgtcgtgccc tacatctaca gccccaacgt caaggtctgc
     7621 accatccagt gggccgtcgg cctggagata gccctcctcg cgaaagaccc gatgcggacg
     7681 ttcatcacca ccgaccaccc gaacgccggg ccgttcaccc gttatccgcg cgtcatcgcc
     7741 tggctgatga gccgggcggc gcgtgacgcg accatgaaga cgttcaagtg gcttgacaag
     7801 acgatcgccg ccacctccat cgacggcatc gaccgcgaac tcacgctcta cgagatcgcg
     7861 cagatgaccc gggcagggcc ggcacgggcg ctcggcctcg cgcacatgta tggcggactt
     7921 gcccccggca tggatgccga tgtcgccgtc tacgccctca accccgagga tatgccatcc
     7981 gatccggcag cgatcgagaa tgccttctcc cggtgcgccc tgctcgtcaa ggacggcgtc
     8041 gtgaccgtcc gggacggaga agtcgttgcg gagcccgcaa agcggacaat ctgggtcgac
     8101 gtgaaggtcc cggagaacac gcaggtgcag agagacatct acgagcactt cctcaggcac
     8161 tacagcgtga aactcgacaa ctactcactc ttcgaggaac acctccataa cccgcgggtg
     8221 atcgaggtcg atacgacaca atgaggcgat aatcatgaag acagtaaccc tcactctgac
     8281 caacccctcc gaactccttc ttgaggggca gaatatcact ccggatacct ttgcgggcaa
     8341 gaaagccgcc gagattgcca ccctcgaact ctgggaaggc aaacagaaga acccgctcgg
     8401 gaagcacttc actgtcgcag gcgacggtgg agcgacggca gccgagacga agatcgtcct
     8461 tcgcggggac tgcacccgtg taaagcggat cggccagcag atgaccgcag gcgagatcgt
     8521 catcgaaggc aacgccgaca tgtacatcgg cggctggatg aaaggcggga agatccacgt
     8581 gaaaggaaac gtcgactcgt tctgcggcat cgggatggaa ggcggcgagc tgatcatcga
     8641 cgggaacgcc ggatgtcacc tgggctcatc accccgcggc gagtggcgtg gcatgaaagg
     8701 cgggctgatc cgggtcgccg ggagtgtcgg cagcgacaca gccacgttca tcaacggcgg
     8761 gacgatcatc atcggcggca acgcggacat tcacgtctcc acccacggcg aagggggcac
     8821 cgtcgtcatc aagggcgacg ccagaggccg tgtcggcggg cagatggtga aaggcgacat
     8881 ctacgtctac gggacgatcg agaacccgct cccggggttt tcgatggtcg gcgaggtgga
     8941 gcaggaggtc gacggtgaga aggccctctt cgcccattac atcggggacc tcggtgaacg
     9001 ccaccccatc tccagaggca agacggtgta tgcaaacctc tacaccagac tctaaccttt
     9061 tttccccggg catgcgatga cctcgaagcc gcaggcgagg ggggcgtgac accatggtga
     9121 ggcgacacct cacacaaaaa ccgctcgttg aggtcctgga gatgatgcgg gccacctttc
     9181 cgcgcccgga ccggaccatt caggccccgg tggccggggc ggccggacgg gcgacgatcg
     9241 gccccgtcta ctcgcctctg acggtcccgg caacggatgt cgccgcacga gacggctttg
     9301 ccgtcgtgag cagcgagacc ctcggggccg gcgatcgcac cccggtcccg ctcaaaaatc
     9361 cctgccgggt gaataccggc aatgcgattc ttcccggcta cgatgcggtc gtcatggtcg
     9421 aggatgtcac cggaggggaa ggctcctgga gcaccaggaa ggccgccttc cccggggagc
     9481 atatccatcc cgccggcacc gagatccgac agggggaggt gatcctcccg gccgggcacc
     9541 tgatacggcc ctgcgatatc ggagcgctcc tctcttacgg gatcaccgat atcgacgtat
     9601 cggccgtgga gatcggcctc ctcccaacag ggagcgaact ggtcccggcg ggcgagcgcc
     9661 cggacccggg agaggtgatc gagagcaata ccgccgtcgc cgcagcatgg ttcgccgagg
     9721 ccggcgccac ctgcacccgc tacccgatcg tccctgacga cccggagctc ctcaggcagg
     9781 ctatcgggaa gggcattcgg gagaacgacc tgctcctggt ctccgccggg tcatcggcag
     9841 gcacccggga cttcaccgcc ggcgtcatcg gtgacctcgg cggggtgctc gtccacggca
     9901 ttgccatgaa accgggaaga ccggcaatcg tcggcaggat cgacggcaaa ccggtcgtcg
     9961 gccttccggg ttacccgatc gccgccctga cggtcatccg ggaactcatc ctccccctgc
    10021 tcgcggaatg gggatttcac gcccggcccc gggagaccct ccgtgcccgg ctcgccggga
    10081 cggtcacggg agatccggcg tttgacgaat ttatcctcct caccgtctcg cggaccggag
    10141 accggtatac cgccactccc ctgcccccgg gagccgggac gcagatgacg gtagtgcgtg
    10201 caaacgccca cctgcacgtt ccggcaggca ccggtactat ctgtgagggc acggaggtcg
    10261 atgtcctgct gaccgggccg cgtgcgctga tccgggaggc cccggggagt cccggacccg
    10321 caccggaaca ccaggtgcgg ggagatgcgg cactcccgct gaaagagagt tcctgacggc
    10381 gcccggtcac ccctcgctct cgcaggtatc catctgggtg cggatgcggg cctcgaggtc
    10441 aggaagcctc tcctggtatt cccgtgcgag caggaacgtc cggacggcgt cgcccatccc
    10501 ccggtggacg cagaggaggt gacggtactc ttcgggaacg ggataccgga agtgcgtcga
    10561 ggcgacggcg gatccgtcga cgctaaagtc gatgagccgg gcgaggcggt cgagggagag
    10621 gacctgtttc atcccgaaac gccgccggat ctcccgctgg aggtcgatca gcatgcagcc
    10681 gtcgagcgag aagtttgcgg cccggaacgc cttcttctcc atatctgctg caaagacgac
    10741 gatagccgga gtatcccctg ccgcgatgac cgcctccatg aaggtcccga ggtccccaaa
    10801 cgcatgccgg gcggtctctt ctgccgccgg cacctcgtcg tcggagaccc ggaaggagtg
    10861 gtcgtaggcc atcccgtact cgccgcgacc catattcgcc acggtcgtcg cgacgccgac
    10921 agttttgccg cacgggtcgg tcaccttctt ccagatctcg acatcgatgt cgtaccggaa
    10981 ctgctcgccc gcgtagtgga cgctgtcatc ctccggtcgg tggacgactg caccgatctc
    11041 gatcggcatt atgactcccc gactggtgcc gtatatgtac ccgaactcga tatccaggta
    11101 gacgcgcctg cctgacccgc ccgtcgcgga ctgtaccaca gtagagatct ctttccccga
    11161 caacgaaaag gtttcgcggc ctcctgtctc ccggaaagat ttatcctgca ggacataagc
    11221 catttataga agagcaggag gccctgcacc caacgaccat gagactctcc gcaatatggg
    11281 aaaaacttcg taacagcggc ttcttcggag agagacctcc agaacacccg gagcccaccg
    11341 gcgatgaccg ggaagcggag gtgcgtgcgc tcctcgcccg ccccccattc gggatcaccg
    11401 atctccgcga cgccgccctc cccctctacg accgggcact gacccaccgg tcgtacatga
    11461 ggaccggaga gtatccggta acggcagtcg acgacaacga gcggcttgag ttcctgggga
    11521 actatatcct tgacttcatc atcgccgacc acctctacgg ggggtacgac ctcccgccgg
    11581 gggagatgaa ccggagactc caggtgacgc ggaacacgaa actcgccgac atcgtcttcc
    11641 ggcagggcct cggcatcgac agcgtcatca ggagacgggg gcaggtgctg accgactcta
    11701 tcatcgccga tgcgttcgag gccctgatcg gcgctatcta cctcgaccgt ggcctcgacg
    11761 cagcccggga agtggtgctc gcgatctttg aaggagagat tgcagcgtgc gacacccgca
    11821 ggaactaccg gggaaggctc caggagtacg tagcccggga gaacctcggc gaactcgatt
    11881 acgccttccg ccagaccgga cccgggaact gcccggtctg ggccgcacgg gtgatcgtcg
    11941 gggggactcc cctcgggaag ggagaagcgc ggaccaagca gggagccgcg atgctcgcgg
    12001 caaaagaggc gctcggccgt ctcggggagg agtgagatgg tgcagccggc cggcagggat
    12061 gcgaccgtcg cggtcgtccg ctgcgacggc tatgacctcg aggaagtcga tgcggccgtc
    12121 cgccgtgcga tcgacctcgc cggcggcata gagaggttcg tctcgccggg ggagaaaatc
    12181 ctcctcaaac caaacctcct ccaggggcag gcgccggaac ggtgcgtcac cacgcacccg
    12241 gcagtcgtcg ctgccgtcgc ccggctcctc acggaacacg ggtgccgggt cgtcatcggc
    12301 gacagccccg gggccggggt cgtctacagc gaggcgaacc tgcggcgggc atacttgcgc
    12361 gccgggtata cggcggtggc ggaggagacc ggcgccgtcc tcaactacga taccagttcc
    12421 cgggtcatct cgttccccga gggcgaggcg atgaagcagt tctccatcat caccccggcc
    12481 gcagaggccg acgcgatcgt tgttgtttcc aaggccaaga cccacatgtg gacccggatg
    12541 accggcgcca ccaagaacct cttcgggctc atcccggggc tcgataagcc ggtcttccac
    12601 ttccggttcc aggacgagta tgccttcggg aggatgctcg tcgacctcaa cgagtgcatg
    12661 aagccccggc tccagatcgt ggacgcagtc atggcgatgg agggggacgg cccccaggcc
    12721 ggcagcccga gaaagatcgg ggtgatcctt gccgggagcg actattcggc cgtggacacc
    12781 gttcttgcgc ggctcatcgg catggagccg ctcgagatcg ggagcatcag aagcgcggcc
    12841 gcgcgcggcc ttctcgatcc cggggctgtc cggacagtcg gcgacgaccc tctggacttc
    12901 gcggtcccgg acttccggaa accctcgacc tacgccggcg ggggggcggg cgtctggcgg
    12961 cgggtggtac tcgccgtcgt ccagcggttc gggaagacct acgctccccg gcccggcgtg
    13021 atcgctccgg cgtgcatcgg ctgcgggaag tgcgaacgga tctgcccggt gcacgcgatc
    13081 accgtcacgg aaggcagggc gacgatcgat ctatcgcgct gcatccggtg ctactgctgc
    13141 cacgagatgt gcaccgagca cgccatcgcc ctctcccggg gcctcaccgg gagactcctc
    13201 gcccgcctgc tcgggtgagg cgacgaagcc aaaacgctca tgagtgatgc cggcagagca
    13261 ggggccatgc catgcccgtt ctgcaacccg gccagggagg atatcgtcgc ggcaaacgat
    13321 ctctgctacg cccgctacga catccacccg gtcagtcccg gccacctgct cgtcataccg
    13381 ttccggcatg tggcgagtta ctttgatacg acagacgaag aaagaatggc gcttgcccgg
    13441 ctcatcgacg actgcagggc gaagaccgag cgtgcgcatc atccggacgg ctacaacatc
    13501 ggcgtcaatg ttggagaggt cgcgggacag aacatcatgc acgttcacat tcacttcatc
    13561 ccgcgctatc ggggtgacgc cggagagcac ggagggggcg tgcggcgcgt aataccgcga
    13621 aaagaagggt ggcggcgggc gtcaggttcc cggagaggga tgagatgaaa tcggccctcc
    13681 atcgtcggaa cacggcctgg gccgggacgg gccagccgat accccggggt gcctatttgg
    13741 aatgattaat tatgtaagta aggcatagtt accttacgag ggactttagt gcagacgctg
    13801 ctcttcctaa tactatttcc catcctcgtc gcgtgtcttt tattgtttgt gaaacagacg
    13861 acattgcgtt acgcggtggt cgccctctcc gctctggcaa tcggcggagc atcgatatat
    13921 ctgcttatgc agtatgcgtt cgagggcgcg gtctacttcg ccgcgccttc ggaggcggcc
    13981 agccttgtta tggtggccat cgaaatgggt ctggcgctct tcatcgttta catgggcgca
    14041 aagttcagga actacctggc aatcgtactg tccgtcgttc aggcggcgct ggtggtatac
    14101 cttgagatat cgttcccgga gaaccttcac gcggtcaaca acctcttcat tgaccagttc
    14161 tccatcatca tggccctgat cgtagggctc atcgggagcc tcatagccgt atacgcggtc
    14221 aggtacatgg agacatacca ccaccaccat ccagaggtgc gtgaccggca gaagatgttc
    14281 ttcttcgtta ccttcatctt cctcgccgcg atgttcggcc tggtcttctc caacaacctc
    14341 atgtggctct tcttcttctg ggagatcacc acgatcacgt cgttcctgtt gatcggttac
    14401 tcggagacgg aggaggcgac aaagaacgcc ttccttgcgc tggtgatgaa cctcctcggc
    14461 ggggtcgcgt tcgtcgtggc gctcatcttc cttgccgcaa ccgagccgac gagcgggggc
    14521 atcgatcttg cccgggtgct cacctcgggg gacgcggccg tcatcctgat cccggccgtc
    14581 ctgatcgggt ttgccggtat cacgaaggct gcgctgatgc ccttctcctc ctggctcctc
    14641 ggggcgatgg tggccccgac cccggtctcg gccctgctcc actcgagcac catggtcaag
    14701 gccggcgtct acatcctggt ccggttctca ccggtcttcg ccgggacatt cgccgggttc
    14761 tccgtcggtc ttgtcggcgg agtgaccttc gtcgtcgcat ccgcgatcgc gatatcacag
    14821 agcgacgcca aatcggtcct cgcctactcg acgatagcca acctcggcct gatagctgcc
    14881 tgtgcgggtg tcgggaccta catgctcgtc tgggctgcga tcctcttaat catcttccac
    14941 gccgtcgcga agtcgctcct cttcctcggg accggggtga tcgagcaccg gaccgggagc
    15001 cgcaatatcg aggatatgga gggcctgatc gtccggatgc cgaagatggc agtgatgatg
    15061 ttcatcggga tcgccggcat gttccttgcg ccattcggta tgctgatctc caagtgggcg
    15121 gcgatcgagg cattcgtcca ggtccccttc ggcctggtct tcatcgccat cctcgcctac
    15181 gggagcgccg tcacggtctt tttctgggcg aagtggatgg gcaaactcgt cacggtcacc
    15241 cggagccagg agaaggtcga gaagggattc ttcgaagagt cctgggcccc cctctacatc
    15301 atcaccggcc tcgtgatagc ggccgtcctc ctcttcccgg tcatatcctc gacgctgatt
    15361 gagccctacg tccttgctat ctacggggtt acggcactcc tcccgcaggc gaacgtcgcc
    15421 atcatgctcc tgatgatggc cctgctcctg gtcctcccgg tctcgttcct cctctttaag
    15481 aggagcgcaa agcacctccc ggtctacatg tgcgggaggc cctcgacacc ggacctgcat
    15541 ttcgccgggt ccctcggtct gacccgggag gtccagacgc agaactacta cctcaccgaa
    15601 tacttcggcg aggcacgact attccgcccc ggagcagtgg tctgcatcat cctgatcctt
    15661 gcatcatggg tccttgcagg ggtggcctta tgacctggac ctggctcatc ggggcggtcc
    15721 tcttcctcat cctcgcgccc gtcgccggcg gcctcatcgc cggtatcgat cgaaagatca
    15781 ccgcgcggat gcaggggaga gtcgggccgc cgctcctgca gcctttctac gacgtcggca
    15841 agctcttcga gaaggagaac gtcgtcgtca ccacggcgca gaacttctac gtcctcgcct
    15901 acctgatctt catcgccctc tccggcgcgt tattcttcgc gggaggggac ctcttactga
    15961 tcgtcttcgc cttcacgctc gcccatgtct tcctggtgct cggggcctac gcggtccact
    16021 cgccctacag ccacatcggg gccgagcggg aactgattca gctgatggcc tacgagccga
    16081 tggtcatcct tgcagtcgtc gggctctaca tggttacgaa cagtttttac gtcgccgata
    16141 ttgcaaccac gaccatacct gccatcctct acctccccgg tgtcttcctg ggcttcgccg
    16201 tcatcctgac gatcaagctt cggaagtctc ccttcgacat ctcgacgtcg caccacgcgc
    16261 accaggagat cgtcaagggc attacgacgg agttctcggg ttcaacgctc gcgcaggtcg
    16321 agatcgccca ctggtacgag aacatcttcc tcctcgggtt catcttcctc ttctttgcct
    16381 ggaacccgat catcggggtt gtcgcagtcg tggtcaccta cgtagccgag atattcatcg
    16441 acaacgtcac ggcacgggtt cggtggcagg cggcactgaa gagtgggtgg cttgcagcag
    16501 ttcttgccct cgcgaacctg gcgatcctct cctatatgat gctagggggt gtatgagtac
    16561 catggcatac ctgaagagat caccctggat ccttcactat gatgcgtcca gctgcaatgg
    16621 gtgcgatatc gaggtgcttg cctgcctgac accgctctac gacgtggagc ggttcggcat
    16681 catcaacacc ggaaacccga agcacgcgga tatcctgatg atcaccggtg ggatcaacga
    16741 gttgaaccgg ccggtggtca gaaacctcta cgagcagatg ccggagccaa aggtcgtcat
    16801 tgcaatcggt gcctgtgccg caaccggcgg tgtattccgt gagtgctaca acatcacagg
    16861 aggcatcgac aaagttatcc ccgtcgacgt ctatgtcccg ggatgcgcgg cgcggccgga
    16921 acagatcatc gacggtgttg taacggcgct cgggattctc gaggagaagc ggacgaagat
    16981 gacgagagac gcgcaggcaa aggaagaagc gcggcacgcg gcaggtgaga gcacatgaca
    17041 gttaaagaag agcagactac catcagcgtt gcgctggagg atctcctgcg ggaggtcaaa
    17101 gggctccagg ccgacggata ccgcctcgtc cagatcgggt gcacggacat cggcggggcc
    17161 tacgagatca actactcgtt cgataaggac taccagttca agaacctccg cctgacggtc
    17221 gggccggata tagaggtgcc gagcatctcc ggcatctact ggggggcgtt cgtctacgag
    17281 aacgagacgc acgacctctt cgggattccg atcaccggga tcaacatcga cttcaagggg
    17341 acgttcatca agtcggggga gaagtacccg ttcagcgtca cgagaagggg gggtgaccaa
    17401 tgccgaaacg catagtcgtg ccgttcgggc cgcagcaccc ggtgctgccg gaaccgattc
    17461 acctcgatct cgtcctcgaa gacgagctgg tggtggacgt agtcccctcc atcggctaca
    17521 tccaccgcgg gctcgaacag ctcgtgcaga agcgcgagtt caccgagtac gtctacgtgg
    17581 cggaacggat ctgcggcatc tgcagtttca tgcacgcagt cggctactct cagggcgtcg
    17641 agcacatcat ggggctcgaa gtccccgacc gggcacggta cctccggact atctggtcgg
    17701 agtgctcgcg tctccactcg cacctcctct ggctcggcct ctttgccgac tctatgggct
    17761 ttgagaacct cttcatgcgg tcctggcagc tcagggagag cgtgctcgac acgcttgagg
    17821 acaccaccgg tggccgggtc atccagggca cctgcaaggt cggcggagtc aggcgcgaca
    17881 tagggcagga gaaactcgac gagatgcacc gggccctcga ccccttcgag gagcagtgcc
    17941 gggacctcgg cgatgtcttc ttgaacgacg atacggtgaa gtaccgtctc aaggggctcg
    18001 gagttatctc aaaggacgag gcctacgctc tcggggcgac aggaccgacc gcccggggga
    18061 gcggggtcgc gatggaccac cgggagaccg gatacgctgc ctacggggat atcggcttca
    18121 aacccgtcgt cgagaccgcc ggggactgct acgcccggtg cgccgtcagg gtcagggaag
    18181 tctacgcctc tatcgaccag atccggcgcg cgatcgatca gatacctgaa ggaccgctcg
    18241 acgtgaaggt gaagggaaca ccgaacggcg agtacttctc ccgcgtggag cagccgcgcg
    18301 gtgaggtcgt ccactacctg cggggcgacg gcaccaaaca ccttgcccgg gcgcgtatca
    18361 ggacgccgac gctcgcgaac atcccgatgc tggtgaagat gctccccggc gtgcaactcg
    18421 ccgatgtccc ggtcgtggtc ctctcgatcg atccctgcat cagctgcacg gagaggtgag
    18481 acggcatggg attttttgcg atgacaaaaa ccgtcctccg gaaccttgca gagggaccgg
    18541 ccacccgcca gtatccagcg gtcccggcgc ggacgagcgc cctcacccgg gggcacatca
    18601 ccttcgaccc ggcaacctgc cgctcctgcg gcctctgctc gcggcggtgc ccgtgcgagg
    18661 cgatccgtct cgataaagaa gagaaggtct gggagatcaa ccgcatgcgg tgcatcgcct
    18721 gcggcgactg tgtcgagggc tgcccgttcg ggagcatcac gatggagcgg gactacctcc
    18781 cgccggtagt ggagttcgtg gtggagcgcc acaccatcac ctacgtgaaa cccgaaaagc
    18841 ccgagaagat accaaaggaa gatagcgcgg gcgagagcgc gtgaagggag gccgggatga
    18901 ggagccccgg tgagatcttt ttttgctatt ttttgactga caactatcga tcagtggaag
    18961 gtccaaaggt tggcaggtgc aatcgcaact gctgaaacag ccgaaactgt aagagaaccc
    19021 cgtacactgg accgtgggaa tatcgccacg gggggaaggg gctgacgggg agtgcgagcg
    19081 agagcgagct tgagcaccga taggtgcgag gggggagcat ccccacgtta caccttcaga
    19141 cttcatccct gcagactgtt ctactcctga cccccacctc tcccgcgctt cgcgctcctc
    19201 ccccgcccca agggcggggg cagtgcgtgg cgatgcccga tggaaagctg tggatgggca
    19261 tacgcgaccg gccaagagtt cagcacctta aaccagtgtg agttacagaa cgtgacccac
    19321 agggtgcagc gtcgtcccct atctctcgac ttcagacaac ttaccccctt tcatttccgc
    19381 accagcggat ggaccatgtg gatgatgtcg taggtgctgc tcgggtatgc ccacgggatc
    19441 gcgtccagcg tcaggtcgtc caccgtcagg ttgtgcctga tcgcgagggc gaagatgttg
    19501 atcacctcct cgacgtgagg cccgatgagg tgggccccga ggatccggcg ggagtcgccg
    19561 tcgatcagca gtttgtagct gacatacttc tgaccgatgc tccggttcgt gaagcgcccc
    19621 gacaggtcgc cggcgttgac gatgtagggt atccccccct ccatcgccgc cgcttccgtg
    19681 agcccgacgg aggcgagggg cggcgtcgtg aagacagcgc tcgggacgac ggagtagtcc
    19741 gcgacccgtg cgttcccgcc caggatgttg tcgacgacga tgtgggcatc catcaccgcc
    19801 accggcgtca gttgcgggct ccctgcgttc gcatccccgg ccacatagac ggcggggttc
    19861 gagacgctcc ggaggtattc atcgaccacg attccccgcc cgtcggtctc gacgtttccg
    19921 gccgcaaggt cgagatcctc gatcgcggag accctgccgg cgccgtggac gaccatatcg
    19981 gcatcgaagg tcttcacctc cccctccctc ccggcccgca cccggaggcc ggcggcgttc
    20041 ctctcgaccg agagaagcgg catgttcacc tgcacgtcga tcccgatctc ttccgacgcc
    20101 accaccagcc ggtcgacgat atcggggtcg aaccctgaga gcacccgccc actccgctgg
    20161 aggatcgtca ccgccgaccc ggccgcggcg gccacgtggg cgaactcgaa cgatatgtag
    20221 cctccaccga cgaagacgat ccgttccggg agcgcctcga ggtagaagaa atcgtcgctc
    20281 agggtcatga ggtccgctcc cgggatctcc cgggggcgcg ggtgcgcacc ggcggcgacc
    20341 acgatatgcc gggccgtgag cgtgtcgtcg ccgatagcca cccggtccag gccgacaaag
    20401 cgggcaagcc catggtaggt gtggatgccc gcctcccgga agcttccctc cttccgctgc
    20461 gggacggggt cggtgaacgt gcgctcgaag gcgaccagtc ggggccagtc gatcacgacc
    20521 tcaccccgga taccgttccc ctgctggtca cggacgcggg cagccgccgc agcggcaccg
    20581 gcaagcaccc tcttcgggac gcagccccgg agggcgcacg tccccccgta atcccggcaa
    20641 tcggctatcg ccacccgcat tccggctttc ctgcagtgcc aggcgatgtc ggagccggcg
    20701 accccggtcc cgataacgac gacatcgtac tcccgctcca taatggggct ccggtcgcca
    20761 tagagcggta taaaggttta cccggaccgt cgggcaacgg taccaccggt cgccctgctg
    20821 gagagccggg aggtcagaag tagagaaagg agacgatgcc gaccgcaatc acgatgaaga
    20881 cgacggtcat gacgctcccc gcgacgacat agtcccgggt ccggtagttc ccgggacccc
    20941 tcaggagggc gttcaccagg tgggtcggaa ggagttcgag gagcgcgagc ccgcggggat
    21001 cgagcccgaa cgagcctgcc atcaccagca caagcggagc caggaggacc tgggtctcca
    21061 tcgggcaccc tatgggaggg gagctgaaaa aggtgcgggg gaccggggat tgaagcggag
    21121 atgccctgga acccgggaaa atcactcgta gatcgcgcgg gtaacccggt gcggctcaaa
    21181 ctgtccacgg tggaagaccc ggacctcgac ctccgcgcct ttcccgaagt cccgcatccg
    21241 gaggtcgtag gtccgccgga cgtagcgcag cccctcccgg acgaagaggt cccggagggc
    21301 tcgccggaac cggatctcct ggtcgaggaa cgctgcctcg tatgcccggg tctcccgggc
    21361 aatccggacg cattcggcgt cgtcgatcgg ggtgttgtag cagccgtgct tgttcaagcc
    21421 gccgatctgc cgccagccga tggcgcccgt ctcgtcggcc tcccggtgga gcatgtaggg
    21481 gtagacccgg cagatcgaga accgccggtc gtagatgctg cacatgccat ccttgagaaa
    21541 gatacaggtc ccttccggtc ctgtccggag ggcatatccc gatacgtaga acctcccctg
    21601 ctgatcgcag gcatcgtaat tgggcgccgg aattatggcg tccggcctaa atgagcggac
    21661 acggtcggtg tcctcttcca gcaggaagac atggccgttg aactcccggg tgcagcaccg
    21721 gccgcagcag tcgcaggaga accccacgtc ccggatgacg tctatgaact cgtcttccgg
    21781 aaactggaga aggtctttta gttcctgctc gagggcttca atctgttcgt cgaatgagag
    21841 cgtagtgggg gcctccgtca ttatgtccgg caggatgcgc cggcaccggt accctcaatg
    21901 ttgaacgggg agggggatat gcccatcggc atccgccgta tcatgacttt ttccccacca
    21961 tcgcaacgta gatcaggaac tgctcttcgc cgccgatgcc gagaagccgg ttcatcgccg
    22021 cgtcgtcaaa ggcggcgatc gcacagaccc cgcaccccac gcttaaagcc gccaggtaga
    22081 ggttctggca gacgtggccg gcgtccaggt gcaggtagcg gtagccgcgc tcaccgtagc
    22141 gccacgtcat ccggtccgga acggcagtcc agaggaaggt gacggcgctc ttgaggatat
    22201 gctgctggtt caggcacccc gccgccactc gcacctgacg gtgctcgaac tccgttcccg
    22261 gagggaacat cgttcctgcc gtttgatccg ggatcgcgcg actcttcggc aaggcggtgc
    22321 tccagcgcca ggtagcggta aagtcccggg ggagcatcct cgacgcggtt gatcagcagg
    22381 taggtctcga acgcgtgccg tgcgcccacg gacgggaccg tgcggagggt gaagatcctg
    22441 tcgacatgcc tgaccccctg ggtgccgatc acgtcccgaa ggtcgacctt cgggatggcg
    22501 atatcgccgg gcgcgggcag cgggatgatc gtctttgcct tcgtgtacgg gagatcgaga
    22561 ggcggtggcg ggcggcccag cagctgggcc gacggtggct gactcccgta ttctgtcctc
    22621 agcgtgaact cctttcctgc cccgcgcagg gacctggcag cccggtgaat gcagaagata
    22681 aataatcttt tcccgccgcg aatcctttcg ctgcaggcgg tcgtccgggc cggactccgg
    22741 aaacgtatta ccggaccccg cccaactaat atcaccactt tcgaggcgtt tgcgtgatcg
    22801 taaccttcat catattcatc gtcggcatag ccctcctcgt gaagggagcc gacctcttcg
    22861 taggcggcgg aagcggcctt gccctccgct acagcatctc cccggccctg atcgggtcca
    22921 cgatcatcgc gttcggcacc tcgctacccg aactcgtcgt cagcacgaac gctgcagcca
    22981 cgggcaactc gggtattgcg ctcggaaatg tcttcggaag caacattgca aacatcgccc
    23041 tcgtcctggc cctctgcacc ttcatacggc ccgatatggt ctccgcgtcc gggacatccc
    23101 gctccgcgct cgtccggcat gctgcgttga tgctcgttgc gacggcggca ttcgccctgc
    23161 tcgccgtgag aggcacgctc gacgccctca cggggggggt gctcctcatc ctcttcgtgg
    23221 ccatcctctt catgctcctg cgggaacgcc gcgaagaaga aggggttgag atcgagtccc
    23281 acggatgggc cgacgctctt tacatcggcc tcggactcgt cgctgtcgtt gtcggagccc
    23341 agctggtcgt caacggcgcc gtcacgctcg cggagatctt cgggattccc gcctttgtca
    23401 tcggtgtctc ggttgtcgcc gtcggaacgt cgctcccgga gctcgcgacg tccctggtgg
    23461 ccgccgtcag aaacgaaggc gccatctcga tcggcaacat cctcgggagc aacatcttca
    23521 atatcctcct ggtcctcggg ataagcctcc tcctcgcccc ggccaccatc gggtcatgga
    23581 tcgacattat cgtcgtcgtc ctcttctcgg tcgcaatcct ccccctgctc tttgcccgcc
    23641 cgtccgtcgt ccggggctgg tcggccctcc tgctcgtcgg ctacgccgcc tacatggcct
    23701 ggatcttcgg ggcggtgtcg gtgtgatgga gtccggaaga cttcgaaaat attcgtaggt
    23761 tacacaaaga tgatgcatcg gcgcgtgcat ctccctctac cggaaggtga acgagtatgg
    23821 ggaactggaa actggtaatg gtcgccgccc tcgggtctct cgtgatcgcc gcgatcatcg
    23881 ggaccgccct cgtgtcggag gacgccgggg cagggaccta tggggactta cagaaattcg
    23941 cgtcaaaaga ggagatcagg gcgttcttaa aggagcacgc gcagggaggg gtgagcgatg
    24001 gtggctactc ctggtccccg ggaggtgccg ggcaggagac cgtcgcgaac gcaccggcgc
    24061 cggcgtcgac ggccgccccg acatcctccg cccgcgacta ctccaccacg aatgtgcagg
    24121 tcgagggcgt ggacgaggcc gacttcgtga agaacgacgg gaagcacatc tacatcatct
    24181 caggggagac cctcgcgatc ctcgaagcgt tcccggcgaa ggacgcaaaa attgtctccg
    24241 agacccggat cgacgggtcg ccgacagcgc tcttcctcgc gggtgaccgc ctggtggtct
    24301 ttgcgaccgc aacagaggag cagatgacca cccccgaagg cagcgtaaca ccggtcccgg
    24361 tctggcggac ggtgacccac gcttacgtct acggtctcgg cgaccgggga gatccggagc
    24421 tggtccggac cctgaccttc accggcgatt actacgacgc ccggatggtc ggggactacg
    24481 tctacaccct cacacgggag tctcccgtct gggtccggga cgacatcgtc ctccccgagg
    24541 tgaggggggg cgaggccccg cctctccggc ccgacatcta ttatccgaaa agccccatgc
    24601 agagctacat cttctacaca gcaagcgcct tctccgtccg gagcgatacg ggcgcccccg
    24661 acgccgagac gtttctcctc ggctacgaca ccaccctctt cgcatcgcag gagaacctgt
    24721 atatcggcta ccggaacaca ggaccggtgt atctctccag gccatcaaca gacagcgccg
    24781 gcatccggga ccggacgatc atccaccggt tcgcgatcga gcaaggaaag atcgactata
    24841 aggccatggg agagattccc ggagatctct taaaccagtt ctcgctcgac gagtatgcag
    24901 gcaacctccg ggtggcgacg accgtcgaga cctggacccg tgatgggtct ctccggtaca
    24961 acaacgtcta cgtcctcgac ccggcaatga agaccatcgg gacgctcgaa catatcgcgc
    25021 cagacgagcg gatatacgcc gcccggttcg ttttggaccg gctctacctc gtgacgttca
    25081 agcgggtcga ccccctcttc gtcatcgacc tctcggaccc aaaacatccg ggtatccttg
    25141 gaaaactgaa gatcccgggc tactccgact accttcaccc ctatgacacc aaccacatca
    25201 tcggtgtcgg gaaagagacc agcgagaacg attggggcgg tgtctcggtg gaagggctca
    25261 agatcgccct cttcgacgtc tcggacgtaa acaaccccac cctgatcgat accgtcgtga
    25321 tcggggaggc cgggaccgat tcggaagccc tccgcgacca caaggccttc ctcttcgcag
    25381 ccgaaaaagg cctcattgtc cttccggtga gcgagataaa gcgggtggag aacccgtcgt
    25441 caagctactc cggctcttac agcaccacga cctggcaggg tgcctatgtc tactcggtga
    25501 gcccggagga ggggttcact ctgaagggca tagtcgccca cgccgagaaa gggccttcct
    25561 atgcctggga ctcgcctgac gcggtgcggc gatcactctt catggacgac accctctaca
    25621 ccgtctcaaa gcggagcgtc gtcatgaccg gtcttgccga cggcagccgg atcaatgagg
    25681 tcttcctccc ctaccggtgg gagacctacc cgaccccgta tccggtctgg tgaccgggag
    25741 gacggtgttt cctcaccgcc ttgaagatct ccatcacgac cacgaccgac gaggcgagga
    25801 cgagcagcga gagccattcg accagggaaa cgggctgcag ctggaggata tcccggataa
    25861 cggggacata gagcgcgagg atatggatac cctgcgcggc gatgacaccg gcgatcagga
    25921 agtagttgct gcttatcggg acacggaagg ccgagcggta ctccgaccgg cagttaaaga
    25981 cctggaagtt ctcgaagagc accatcagca ggacgatcag gttccgggcc gcgaactcgt
    26041 cccatccgtt tgaaaggagc cagtaccagg ttccgaccgc caccagcgcg atgacggtac
    26101 cggagatgac ggtctcctcg atcatcagcc ggttgaagat cccctcctcc ggcctgcgcg
    26161 gcggccggtt catgacgccg ggctcgcctc gctcgaacgc cagcgccacg tcctggatac
    26221 cgttcgtcac caggttcagc cagaggagct gaaccgcgag gaggggcagc ggcagaccca
    26281 cgatgagggc gagcatgaag aggagcacct cggcaaaccc ggttgatatc aggaggtaga
    26341 cgactttgcg gacgttgtcg taggcgtacc ggccctcctc gatcccggcg acgatcgagg
    26401 cgaagttatc gtcggtgacg atcagtgacg ccgtatcctt ggcaacgtcg gtcccggaac
    26461 ccatcgcaac cccgatattc gcgctccgga gcgccggcgc gtcgttgacc ccatccccgg
    26521 tgaccgcgac gaaggccccg ctctcccggt aggcctcgac gatccggagt ttctgcagcg
    26581 gggtcacccg ggcaaagacc cgggcggcct gcacccggtc aatgaactcg gggaacgtcg
    26641 cgatctcgcc gtctcccagt tccgcgccgg tgacgacctc gtcgggagag gccgtgatct
    26701 caagctcgcg ggcgatggca agggccgttg cggggtggtc cccggtcacc atcaccacgt
    26761 cgaccccggc gttgcggcac cgccgcacgg catcggggac gtcgggccgg atcgggtcca
    26821 tgaacccgac caggccgagg agttcgaggg gagggagctc ggggttctcc gcagagaccg
    26881 gtccctctgc gcggccgtga gccacggcaa gcacccggta tccccgggag gtgaggccgt
    26941 cgagttcctc ctgcacgtgg tccaggtcga tggggacacc cccctcctct cccgcggcca
    27001 tggtccggca gaacgggagg accgtctcga tggccccctt gactgccacc cggatctccc
    27061 cgccgtcctg gtaccagacg gcggcgtacc gccgctcgga ctcgaagggg atctcagcga
    27121 cggcaggcgc actctcgcgg ataccggcgg ggtcaagccc cagcttgtag gtgagcgcga
    27181 ggaacgccac gtcgatcgcg tcgccctggt gtgcccagtc gtcctcgccg gtccgctcca
    27241 gggcggcctc gttggtgatg gttgcagccc gggccaggcg ctccaggcgt ttacgggcag
    27301 agccggctac cggggctccg gtctcgtcga gaacctcccc ctcgcctgcg tatccggcgc
    27361 ccgtgacaga gaacgactcg ccggtcggca gactgacgac ccgggcggtc tgctggttca
    27421 ccgtgagggt gcccgttttg tcgctcgcga tcagcgtgca gctcccgaga ctctccacgg
    27481 ccgcaaggaa ccggacgatg acgttccgtc cgaccatccg tgacgtcgcg atcgagagca
    27541 cgacggtcag ggcaaccggc aggccctcgg ggatcgctga gacggcgaga gcgacggcga
    27601 ggaagaagac ctcgataggg ggcatcccct ggccgaggac gatgatcgca agcagtccgg
    27661 tggcggcaag gacggcgatg ctgatcttcc tggagaaatc ctccatacga atgacgaggg
    27721 gaggcttgcc cgcctccgac gccgtgaccg tctcggcgat ctgcccgatc tcggtatgct
    27781 ggccggtcgc gaccacgatc cccatgctcc ggccggtcat gacagtgctg ccggcgtaga
    27841 gcatgttcag gcggtcgccc agggggaggg ttgcgcccac gggatcgggg ctcttcccga
    27901 ccgcgtggga ctcgccggtc aggagcgatt cgtcgacggt gagcgaccgc gccgtgaggg
    27961 tgcgggtatc ggcggggacg cggtcgcccg actccaggac gacgcggtcg ccggggacca
    28021 ggtcttctgc cgggacggtc gtctcccgcc cgtcccgcct cacccgtgca cggatcttga
    28081 gcatctgctg cagggccgtc gcactcttct ccgccttcac ctcctggaac gtaccgatga
    28141 cggcattaag caggatgacg acgaagatga aggccgcgtc ggtgacgtcg gcgaagagga
    28201 gggagatgat cccggccgca aggagcacgt agatgagcgg gctcgtgaac tgccggagga
    28261 agacctcaaa gaccgtcggc ggccgcttcg ccgggagggc gttctccccg aagacctccc
    28321 gccgccgggt gacctccgcg tccgtgaggc cccccgtcgg cgatgcgagc cgttcggcta
    28381 cgtcgcccgc aggaagggcg tgccatgccg tcgtccggga ctcgtcgggg gagaggaggg
    28441 gttccatgat atcatctcac gaggtatacg tcgcagggtg ccgatgcccg ggagaataga
    28501 tataaccgac cgtgccggac gtcctgtttt cgaggaggta ggacggtttg gtggaacggt
    28561 tccctcctgc gaggctcgaa atgccgccga tagttatctc cctggatatg accggaaagg
    28621 atctctctcg cccgcatata aaaacctgcg ggcaggcgac ccgcgagagg ggtcacgcgt
    28681 ggatcatcgt cagcatcatc ttgaaacggg tgacggcatg gaccgcatgg ggtttatgtg
    28741 ccggcatgat gatcaggtcg cccgtcttca gggaatattc cgtgcccgcg atggtgacga
    28801 gggcctcccc gtcgacgacc tggaggacgg cgtcgtaggg tgcggtgtgc tccgagaggc
    28861 cttcgcccgc atcgaaggca aagaccgtga tcgtgcccga ttttgtgtag gcgagcatcc
    28921 ggctgacgat tgagcccggt tggtaggcaa cgaggtcgcg gggatcgatg acctgttcag
    28981 tgagggtttc tttcttcgtc tcaggcatac tggccggttt ctcctgcagg gtcttttgct
    29041 ttgcgctccg gggtgacgat cgggcatacg acatcttaac cctcaaggac ggactgaaga
    29101 cataacttgc ttaacctgat taccgcccga tccactgtga gaagaatatt ataacgagtg
    29161 gatgaactgt taatgagtca actacccccg gttaaaa
//
LOCUS       DAJB01000124            7017 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_12341, whole genome shotgun sequence.
ACCESSION   DAJB01000124 DAJB01000000
VERSION     DAJB01000124.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_12341"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    4044..4075
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1178 a         2304 c         2170 g         1333 t
ORIGIN      
        1 gagattgtaa gcggatccag atgcgaaacc cagatatcga agccattaca ccccgggtgc
       61 tgaacgatca cgtaataccg gccttctgtg agattactgg tgttagcgcc tgtaagaacg
      121 tactcgaagg tgccgtcgct ctccaccgtc gcggctccag aatggtaata atccgggccg
      181 aggatccaga cctggacggc agtcggcgct cccgttgcaa tcccgctaac ccgatagtcg
      241 tcacccgggg taagggtggc gccggatggc tgtatagtga gagagggagc cctcagcacg
      301 atggatgtcg tggcgagcct gacgccggtg aggtttccct tgccttgagg ttgtgcagcg
      361 gcgtaaatcg tgtagccgct ggcattaaga cgcctgccga cgtcggcagt gttccacttg
      421 taggaccagg tgtcgtcggc ctcgacgtac acgcgggtga aggtcgccgg gtcgccgttc
      481 tccaccaggg tggtcacgtc cgagagcctg acgccgttgg ccgggaggtc cgggccggtc
      541 aggaagaggt aggtggtgtc actgtcgctg ttagttccgg agagggtgat ctcctcgccc
      601 aagtagtata caccggtgcc ggaggcggtg atggtgacgt ccccccgctc gatctttacc
      661 tcgacgtcgt cgctgactgt cagatctgcc ggatcctcga cccggatcgt gaaggcccgg
      721 gtatcggtgc tctggttggt gttaaactgg atagatcggg tgccagcggc attggtccga
      781 acggttacgt cgatggggct attgctcatg acgccgacct ggccaagtgc aaccaccggg
      841 tactctccgg ggccaaggcc gtcgacctct tcgacgaaaa ggtggtaatc cttgattggc
      901 tcaccggtga tggtcgcgac gaaactgtgt ccacgaatga ctacatcctt atttgacaca
      961 atagcgagcg ccccggagat tggcggggtc tgtgtcgggg ttggtgtcag cgtttgcgtc
     1021 ggcaacgggg tcggtgtcgg tatcggggtc gggtaggccc cgaccgggat cacttgggag
     1081 agagcctcca ccccgtcgac gtagacagtc accgtcacgg tgcccgaagt gatcccctcg
     1141 atggcaaagg agatcgtgct gccctcaggc ccctgcttcg tcccgagcac cgtgagatcg
     1201 gcgacgatgt cgctcgccgc ggcctccccg cggagggtga tggcaccaaa ggtcccgctc
     1261 aggaagttgc ggggctgact gatccggacc tcattgttac tgtcggcgtt gtagcgcatg
     1321 atgacggagc ccccatcagg gagttccgcc acaagctcgg tccaggtcgt gcccctcgtg
     1381 taggcgttca tctcaccgga attgagggag aagggaaggg tgaggtcccg gatggtgaat
     1441 gcaaacgctt ctcccggagc cgcggcgaac tcgccgcgga tgccgagcga gaaggcagcg
     1501 ccgtcgggaa ggccgcttac attcaccatg accgtatccc cgggcgcgat cgcgtcaggg
     1561 ccgatattga tcgttgccgc ggtcgcggcc gggatcagca gaacaagcag gagcaaaact
     1621 ccacccagtc tcttcatgag tagcagctcc tcatttttgt cccaacagag gtgccggtgc
     1681 tattcgccgt gacctcccct gccgtagccg gtgtaatgaa cagcgcgacc aggaaggcca
     1741 cggtgacgat cgccagaaat gccgttcgct cagaattcat cgtaatccca ttctcgacgg
     1801 tacgtaccag gaagggctgg agttccgcag gatgctggct gcgggggggt tgaacgcaaa
     1861 tagaatcgct ggaccagcga aagccctctt cccgtgcgca gggttctgtc ttgtgtaagg
     1921 taggataaac atgccgctat cgtccggacg gacatatcag acacaggcgc aatccaacac
     1981 ccggggaggc ccggatcata gtaattttga tggggatgac gtctgtcgtg agcagtataa
     2041 caccggcact cgcagcggat cagggtgcag gacatcgtct gccgaaccga cacctgcccc
     2101 tatggtctgg tggtacgact ccgcctaccc agggcgatgt cacaaccagt tagaccacga
     2161 gcccaccggg gggcgtccgg caccctacca cgagagcgcc ccggcagggc aacctatatc
     2221 ccgcgccccg cactctcccg gagcatgccc gccggtgcac cgcagtggag gaggagcctt
     2281 cccgccgacc tcgtcgccgg ggcgaccacc gccctcgtcg gcatcccgca ggtgatgggg
     2341 ttcgccctcg tcgcggggat caacccggtc tacggcctct acaccgccac gttctcgacg
     2401 gcgatcgcgg cattcctcac cggctcctcc tacctcaagg tgatcctctc aaacgtcctc
     2461 gcggtctcga tctactcggt cctcgccccg gtccccgagg ccgacgtccc ggccaccctc
     2521 tttgtgctga cgctcctcgt cggactcttc cagctcggct tcgggctcgt ccgggcaggc
     2581 agcctgaccc ggttcatttc gaacgccgtc ctcaccgggt tcgtcatcgg ggcggcgctc
     2641 ctcatcatcc tcgggcagct cgggaacctc accggctatg aaccccaaag acacgcagtc
     2701 caggccctcg tcgtgctcga cctcatcggc cacgcgggcg agatccagcc gcaggcgctc
     2761 gcggtcggcc tcctcaccat cgccctcgta ctcgtcttcc ggcgggtgcc gcgcctcaat
     2821 ttcgtcgccc tcctcctccc gatcatcata gcgaccctcc tcgcccgggc cgcattcccg
     2881 gatgtcgcgc tcgtcggcga tatcagcacc atccccgccg gcctcccgat acccgccatc
     2941 cccaacctcg ccctcgcgcc ggggctcctc gtcccggcgc tcgccctcgc catcatcggg
     3001 ctggtcatgg ccgtcggggt cacggagacg acccccgagc gcgacggcac catcgccgac
     3061 gtgaaccgcg atttctccgg gcaggggatc acgaacatcc tcgcgagctt cctccagtgt
     3121 gcaccggcgg ccgggtcgct ctcggcgacg gcgctcaacg tcagcgcggg ggcagagacc
     3181 agggcagcga acctcatctc gggcgccctc gtcggggtga tcatcgtcct cgcgggaccg
     3241 ctcgcggagc tgatcccgct cccggccctc gccggcctcc tgatcctcat cgggatcgaa
     3301 ctcatgtacc agccccggga gatagtctgc atctgcaaat actcccgctc cgggcgctgg
     3361 gcgatggtcg ccaccttcat ctccacccag gtcctgccgc tccagtacag catctacatc
     3421 ggtgtcttcc tctcgctcgc catctacctg gtcacctcca cccgggaggc aaccgtggtc
     3481 cgcctcgtcc cggcaggagg ggggatgttc cgggaagaac cgccgcccgc ccggctcccg
     3541 accggcgatc tcaccgtcct ctcggtctcc ggcagcgtct acttcgccac cctccgggcg
     3601 atggagcgat cgcttccatc tcccgaggga gcagaggacg ccgtcgtggt cctcgccctc
     3661 cgggggaggg tcgaggtagg gagcgggctc ttccggatgc tggagcgcta cgcccggcag
     3721 gttgcagccc ggaggaaccg gctcatcctc gccgaggtcg acccccggct tcttgcgggc
     3781 ctcgaagaga ccggcgcggc cgcagccatc ggccgcgaaa acatattcct cgcgacgccg
     3841 gtcatcgggg agtcgctcat ggaggcgatc cgggcagcgg aggcactcgt ccgggagaaa
     3901 ccctagagcc ccacccagaa aactcttttc ccttcggagc cccataccgt ctctataagt
     3961 atgaccgccg aaagcctctc cccccctgcc cggatcgcaa tcgtcggcgc ggcggtcgtc
     4021 atcgtcctcg cgggtatcca ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttg
     4081 ctgccggaac cgtggtcgtg gggctcatcg gtgtcctttc cggggtgatc gcctttctcg
     4141 gtgtggcctt caccgggttc atccgctccc tgccgcagta tcaggcgagc ctcgaggcac
     4201 aggctgccgt cctcgccgat tacggcatcg accccggcag tttcaccgta tgggactaca
     4261 tcgaccaggg gttcgtgatc cagcaggtag ccgggctcgc ccggcagatc gggaacatcg
     4321 ccgttgacgc cttcatcgtc ttcgtcggga tcgggttcct cctcctcgag gcgccccggc
     4381 tcgcggcagc cctcgcccgg cgcctgggcc cacagagctc cccctaccgg cacttctcgc
     4441 agtcgagcca gctcctcatc gattacgtgg tggtcaggac aaaggtgaac ctgatcaccg
     4501 gcgtcgggac cgggctcttc ctcaccctcc tcggggtcga ctttgcggtg ctctgggggt
     4561 tcattgcctt cgtcctgagt tacgtcccct acatcggcct cgttgtagcc gccataccgc
     4621 ccaccctcct cgctctcatc gagttcgggc cggcgggggc cgtggccgtc gtcaccgccg
     4681 tcgccctcat cgacgccgcc gccgagaacc tcgtcctccc ccggatggcc gggcgggaac
     4741 tcaacctctc gccgttcgtc gttctcttct ccgtcatctt ctgggggttc gtcctcgggg
     4801 cgatcggggt cttcctcgcc ataccgctga cgatcgcagt gaagctcctc ctcgagagct
     4861 gggaagagac ccggtggatg ggggagttgc tgggcaacgg ggggcggcgg gagtaaggtc
     4921 caggccgacc gcaggagata tatatcctca attatatttt tggctgcatc tcaattgcag
     4981 tggaggatct atgagtgatc tgattgccat cgcgtacgat ggcgaggata ccgcgttccg
     5041 ggtcagggac aggctcgttc agctgacaaa ggagcatgtc atcgaactgg aggacctggt
     5101 ggtcgccgtc catcaccatg atgggaagac cgagatcaag caggccgcga acctggccgg
     5161 catgggggca ctctccggcg ctttctgggg gctcctgatc ggcctgatct tcttcgcacc
     5221 gatcttcggg ctcgccatcg gggcgatcgc cggagcagtc agcgggcggt tcgctgacta
     5281 cggcatcgac gacaaattca tcaaagaagt cgcggagagt gtcgggccgg gcaactccgc
     5341 ggtcttcctg ctcgtcaaga agattacgcc ggacaaggtg gtcgacgcca tcagggagta
     5401 cgggggccgt gtcgtccgga cgtcgctctc ggatgcggag gagacgaacc tccgcgacgc
     5461 cttcggcgcc gggacggcgg caaaggcgga ggtcccgcca cccggtgcgt aagcggccgg
     5521 gtggagacgg tggcaccgcg gagacctgca tgcaccgggc caggtatgag ggaagtcggg
     5581 gtcacccgca cggggcggga cgaggcggta gagatcgcgt tccggttcaa agcgacggag
     5641 cagctccttg acccggacga ccggtctcct ctccccgcgc aggaactcac tgagttcgcc
     5701 gaggagttta tcgccggtcg cctcgagggc tacaacatca aacggatcgc cggcatcact
     5761 gtcggtctcc cgcgggattc gctctcaccg gaggaggcag cgctcctccc ggagacgatc
     5821 cggcggcact tctcgttccg attacgcgac ctcgacgacg acatgcgggt ctcccgccgg
     5881 gaagggagaa tcagtgttct tctcgccggt atcaacgcgg cgatcgcaat cgttttcgtc
     5941 gcgttcttct ccggatccct cgatagtctc atcatcttcc tgctcggggg gttgatcacg
     6001 atcctgaact gggtgacggt ctggcacacc tatgagtact tcgtctacga ctaccggagc
     6061 gagatgcgaa aacgccggat ctaccggaag ctcgcgggca tcgatatccg ggtagaggca
     6121 tggtgacgca ggagagccgg cccagggacg gcacaagatg tttacgaaac gccgccgata
     6181 gtacctcatg cagagacctg ccgggctcta cctcgcctac ctcttccagt tcctcatcgc
     6241 tgcaaacgtc ctcctcgcgc tctcgctcgg cgaatactcg caggtcttcg gcggcgtcat
     6301 cgcgctcggc ctgaccctgg ttcccgccgt cgtcacccgc cggtggagca tcacgctccc
     6361 ctggggggtc aacctcctcg tcgccctctc gctctacctc cacatcgcag gcgagatctg
     6421 ggggttctac gtgatctact acccctacta cgacaaggtc gcccacctca tctccggggt
     6481 aaccgtctcg gtgctcgcct tcgtcatcgt cctgttgctc gaccggttca gcaggctgaa
     6541 cctctcccgg tggatgatcg tcgggttcgt catcatcgcc gcgatggcga tggaagggtt
     6601 ctgggagatc tacgagtggt tgttcgacac cttcctcggg acaaacctcc agtacggtct
     6661 cgacgatact atgctcgata tgatctccgt cctcatcggc tcgatcggcg tcgcattcgc
     6721 cgggaaccgt tacctctcgc ggtcctcaaa ggaggagatt accggaagga tggtagtccc
     6781 cgaagggtcg ggagctcccg gagagtcggt tgaccacgaa gagccccggg ccgggtgacg
     6841 gccggaacct ttttctgatt cgccccggag agggggcgcc atgcttgagc agatggagac
     6901 gggtgcagag aatgtcgtcg ggttccggtt caacggcgag atgactgccg gggactacaa
     6961 cggggcgctg attccggcac ttgagggagc aagaaagaac catccggtcc tcaggat
//
LOCUS       DAJB01000125            8916 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_20192, whole genome shotgun sequence.
ACCESSION   DAJB01000125 DAJB01000000
VERSION     DAJB01000125.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_20192"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1561 a         2794 c         2870 g         1689 t
ORIGIN      
        1 gtgacaggat gcactattcc ctgccctcaa acctgaccag cgcaggtgac gccggccttc
       61 cgacgagtgc cagggcatcg gatatgcggt cggtgatcgt aagatcgccg ccggttctct
      121 cccggtactc cccggcgagg caggcggtgt ggtagcggta tgtccggtcc cgggtgatga
      181 agatcaggtc ctctgcgagg tttgcaagca gcgtctccca gatgagctcg tcgccgatgg
      241 tatccttacc catgctcttt ggggggttgc cgaggagttt gcggcggtgg gcccgcgtga
      301 cgagttcatc ggtccgatgg atgacccgca cggcggggtc gccggagaga tcggtgaacg
      361 cccgggcgat cgggtcggcg gcgggctctc ttatcatgct ctctgtgggt atggcggccc
      421 ccttaacggg cacgatatgc attaagatgg ggaagttttc ttcgttttct cggaacaggg
      481 gtaacaatac cgaatcaaaa tgcttaagaa ttatatcagt tcatctctcc gtaataggtt
      541 gggagcatgt tccgtattct cgtggtcgat gatgagccga cccttctcga actgaaccag
      601 atctacctgg agcggtccgg agatctgcag gttgagacga cgtcatcggc agtgcagggg
      661 ctcgacatgc tatcgcgagc cccgtacgac gccatcgttg ccgactacga gatgccggag
      721 atgaacggta tcgcattctt aaaagaggtc cgcaacaggg gcatggggat accgttcatc
      781 atcttctccg ggcgcggccg cgaggaggtc atcatcgagg cgctcaacag cggggcggac
      841 ttctacctcc agaaaggcgg caacccctcc gcccagtttg ccgaactccg gaacatgatc
      901 ttccaggcgg tcaggcggag acaggcagag gccgaggtgc agcgggccgg agacctgtac
      961 cggagcatct tcgatcatac cggctctgcc accatcatca tcgagagcga tatgacgatt
     1021 gccctcgcga acagcgagtt cgagcacctt accggccact cgcgtgagga gatggaggga
     1081 aagatgccct ggacggcatt cgtcgnngga cctatgattt ccggctggtc gaccggcacg
     1141 gcaggcgaaa agatctgcac atgacgatcg ggattattcc ggggacggat cgctccgttg
     1201 cgtcgatgat cgatgtatcc gaccggaaac ggttcgagga cgagttgagc gcggcgcacg
     1261 agcagatgac cgcagcgttt gaggaggcga aggcaagcca ggagtcactc gcggcgcagt
     1321 gccgcgagat ggaggattac caggcaaacc tccggggcat catagatttc cttcccgacc
     1381 cgacgtttgt gctcgaccac acggggagga tcaccatctg gaaccaggcg atcgagttga
     1441 tgaccggagc ccggaagagc caggtcatcg ggtccgggcc ggaagccatc tgcgcggcga
     1501 tgcccgggtt acggtctccg ctgcttgcag agacgatcct ctcccggggg tccggggagg
     1561 gtgtcacccg ggaagtctgt gtcacctcgc tccgcaccgg ggaggaggtc tgcctctcgg
     1621 cgaaggcatc gccgctctac gatgcacagg ggaggctctc cggcgtgatc gagtcgctca
     1681 gggacatcac cgagtcaaaa cagatggaag cgcggatccg gaaccgggta gacctggaac
     1741 gggtggtgag ttcgatctcc gcgcgattca ttgccctgaa ccccgcagac ctcaaagacg
     1801 ccctggagga gacgctctgg gtcctcgggt cgttcctcga tgtcgaccgg agttacatct
     1861 tccgcttctc cgccgacctc acccgtgcgg agaacaccta cgagtggttt gcagaaggaa
     1921 tcaagccgga tatcggagtg ctgcaggatc tcccgaacaa catgatctcc cggggaatgg
     1981 agctgatcga ggccagaaaa ccgatctgca tccccgatgt ggccgatctc ccggcagagg
     2041 cggcggagcg tgactttctc atgggatacg gggtccggtc gctcctcctg gtgccgattg
     2101 cgaccgctga cgagctcctt ggggtcatcg ggtttgagac catcctgcag acgcggccct
     2161 ggtcggacga ggatctctcg ctgctcacga tcgtcggcaa cctcttcgcc gacctcttct
     2221 ccgggattcg cgtgcacgag cggctccgcg agagtgagga gcggttcagg acccttgttg
     2281 agcggtcgca cgactgttac atccgtgcaa ctacgacgcc gctggcgatc gagtatgtca
     2341 gcccctcatg tgagcgcctg atcgggtaca cacgggagga gattctgagc gatccggggc
     2401 tcatcggaca gtcgattcac cctgacgacc gggagaccta cctgaccctg ctccgggagc
     2461 gggatgcatg tgccagccag ccgtatgcct tccgggtacg ccggaaagac gggcgctaca
     2521 tctgggttga ggtctgcacg atccccgtct acgggaacga cggacaggcg gtcgcgatcg
     2581 attacgcggt ccacgacatc gatgcctgga aacagaccga ggcggcgctc atccaggcca
     2641 acaagaagct caccctgatg aacagcatcg tgcggcacga tatcttgaac caggtcacgg
     2701 tggtgctcgg ccatatcgcc ctcctccggg accggccgct cgacccggac gtcgccgcag
     2761 ccctcggaaa gcagcaggcc gccgtcgaga taatacagtc gcagatcgag ttcacgcggg
     2821 attaccagga cctcggcgtc cactcccccc ggtggttccc cgtcgaaccc ctggtcatgg
     2881 cggcggtaaa agcgctccgg ccggccggga tccggctcgc caccgacctc gacggagttt
     2941 ccgtctacgc cgatccgctc ctctcgtctg tcttctacaa cctcctggag aacgccatgc
     3001 ggcacggcag gaccgtgacg gagatccggg tcacggccgt tctggacggc ggcggtgccc
     3061 ggattgtctg ggaggacaac ggcgtcggcg tccccatcga gcataagcag cggatattcg
     3121 agcggggttt cgggagccac acggggctcg gactttttct cgtaaaagag gttctctcga
     3181 tcaccggggc caccatccgg gagaccggcg agcccgggaa aggggcaaga ttcgagatta
     3241 cggtcccgga gggcaacgcc cggtacgggt aaaggggtcg gatttgcccc tgcaacttcc
     3301 cgccggcgtc ttttggatcg gccctatcgc cgcccgtatt tcctgaacat ataccagagc
     3361 cctgcggcga ccccggccat caccaggagg ccgagcaccg cgacgtagga ctgctctttg
     3421 accacgatcc ggtactcgtc ctccgcttcg tcctggtcgc tcttcaccgc ggcgacgacc
     3481 ttgtactcgc cggcgacgat atcggccggc gggactaccg tcacctgcac cgtcgcccgt
     3541 tcgccggcct caaggctcgc gaccgaggcc gggtcgacgg tcacccgcca cccttgcggc
     3601 gcgctcatgt tgacctcgat gttcgtcagc gctcctccgg tgccggcgtt cgagatcgtc
     3661 atatcgaacg tgagggtgtc gccgcggtcg atatcatggc ggtaactctt tgcgtaggtc
     3721 cgcatgtcgt aggacccccg gagccggagc gtcaggttct cctcgtactg ccgggcggag
     3781 gagccgatca ccgccgtgaa gttgtactcg ccgacaccca ccgagtaggg cgggatgaaa
     3841 tcgaggtaaa gggacttctc ttcaccggaa gggacgtaaa tctccgagac ctcctcggtg
     3901 gcgtcacggc tctccttgaa ccgggcatac cactgctcgg ggaggccgag tacggcgagg
     3961 gtgtaggtat catcgtttct gccggcgttc ttcagggtca tctcatactg cgggttcgag
     4021 ccgatcgccg ccatcctgga tggggacttc accctgagtt cggcaaagaa gttctccttc
     4081 tcgagcggca cgatgccgag atcggtcgtc ttaccgatcc ggacctcgac atcctccttc
     4141 tcccacgccc ggtagccggg ccgggtgact ctgacggtgt aggtgcccat cgggatgccg
     4201 atggagacct tgccctcggc ggtcgcgagc atcccctcga cccgggtatc gccgtcgtag
     4261 gcgctgacat cggcgctctt caccacgttt ccttccttat ccaccacggt ggcctcaagg
     4321 gttcccactt cgccgctgtg ggtcttcgtg accctgacgt agagccagat cgtgccttcg
     4381 ccgatgccca cccggatggg gtactcccct accgcggcat ggccggaggt ctcggccacc
     4441 aggcggacgg tctttgtctc gccggccggg atcagcatcc tgtagacgtc gcggtcttcg
     4501 gcctcgaacc ggatcttcca gttgtcggtg ccgcggtagg tccagtagtt gaggaggcat
     4561 gttcccgtat ctccccggtt cgtgaccgcc agatcgaaga cggcggtgtc gccggcttca
     4621 atcatctcgc ccgggaagcc gcaccggatc tccgtcgaaa tcgtcccgcc ctgtgccgcc
     4681 gccgctcccg gggccatgag gagggccagg aggacaagta ttcccaatct tctgacagtc
     4741 gtcgtctcac ctgatatcca tccgcatgaa cttcacgtag gtcgcggcga agaagaccga
     4801 cgggaagacg atgagcgcgg cgatgttcat ccagacccgg ccgagggcgt cggcaaggcc
     4861 ggtcggctct tccggtgccg tgtcgtaggg cgagtaggca atcgcggcaa tctgcgggtt
     4921 ggtgaccgca atcgcgacgg tgaagtagtt catctgcggc gagaggaggt tcgagacgtc
     4981 gctgatgaat ctccgtttct cgacgtaggt cttcatcgcc tcttcgtagg ccgtccactc
     5041 cgggtcttcg gttccgccgt ataccacgct ctgcgaggcg ctgctgacga aatacccgct
     5101 gctcgcggta cgcacgatct cttttggcat ggggcccatt tcaggcggct cgggcgggtc
     5161 gcccgcgaaa gcgcctgcgg ccatcatccc gaacatgggg aggagcgagg agacggcgaa
     5221 gaagatcacc agggtgtaga tcaacgcgtt cccgctctct tttgcgaccg tcgacatcgt
     5281 gagcgcgacg gcgaagtagg caagcaggaa cccgagcgag accgccccga agatcaggat
     5341 ggcggcgaac tcgtccaccg tcgggacgac cgagaagacg aggagcatgg cgatggcgat
     5401 ggcgagtgcg agcgccatgg caaatcccag ggcggcgacg cccccgagcg ccttgccgtt
     5461 gatgatctcg tcccggtaga ccgggtggga gaggagcgac ttgagcgacc gggtctcctt
     5521 ctcctttgag accagatcga acccgatggc aatggcaagg atgccgccaa gcatcgtcat
     5581 gtaactcatc atggatataa agacgtacat gatcgagggt ttctggggca tccagctcga
     5641 atacgggtct tcgatctcct gcatctgctg gagctgctcg gtatacgact ccaggctgtc
     5701 gttatactgc ccgatgcctt cgtagatgct gatcgtcgat atcacgagaa gcagcgcgag
     5761 gatgatgatg aaccgcctgc tggtgatgtg gtcggagaac tccttccgtg ctacgttgaa
     5821 caatcgatcc agcgccatca tgtcacctcc ggtaagccgc catgaagacg tcctcgatct
     5881 ccggctcttc gacccgcatc tcccggacgt gcaatccctg cgaaaagagg gtttccgtga
     5941 gccggtcccg gatatcggtc tttgcccgga tgaccgcacg attcccgctc ctctcgatgg
     6001 cgatgatatc ggggtcctcg agcgccggca ggtgctcccg ggtctcgacg acgatccgga
     6061 cggtgtcgcc gtccggggat gcgagcgtcc gggcgacctc ctcgagcgtc ccctgcgcca
     6121 caagttttcc ttttgcaagc agcccgacgg tccggcagac ctctctgacc tcggagagga
     6181 tgtgggagga gacaaaaacc gtctttcctt cgccggcgag ggcgaggacg agttcccggt
     6241 actcccggac gccctcgggg tcgaggtttg ccgtcggttc gtcgaaaaag agcaccttcg
     6301 gatcgttgat cagcgcctgg gcgagcccga gccgctgctt catgccgcgg gagtactctc
     6361 cggccttctg ggtgacgccg tcgaggtgga cgagttcgag cagttcggcg atccgctctt
     6421 tgcgcttcct cgcgtccatc ccgtagaacc gggcgaagta gtccaggttc tgctcgccgg
     6481 tcatgttccc gtagaacccg acgtcttcgg ggaggtagcc gatgatctcc ttcacgttca
     6541 gcgggtcttt ggcgacctcg atcccgtcga cgaagcaccg gccgccggtg ggctcgatca
     6601 tgccggtgag catcaggatc gtcgtgctct tccccgctcc gtttgggccg aggaagccga
     6661 atatgtcgcc ttccccgatc tcaaggtcca gaccgtcgac ggccgccttt ccgttgtata
     6721 cttttgtgag gttctccgtt cgtatcattg cacacctcgc cgtgccagtg tggcggcgcc
     6781 tgttgcagac tttgatattc tcttattaac gttttctgtg acaagcgtct actcttcgga
     6841 gggggatcat gattccagca gttgcgaggt catggttctt tcgaaggcgc ccggtgatcc
     6901 gccgtaaatc cggcgactgc ccgcgtaccg ccccccgcgc ggcgtgagtc ccgggaccgg
     6961 gatactctgc agcgacgccc caatgaggat gcagccctgc tcgctcatcc gtcaggcagt
     7021 ctacgccaga gtatgggcgg gagcgggaga taaggcgatg gttaactacg aaaaaaatcg
     7081 gacctataag aagggccgca atgcaaaaac cttttctccc gtcggtctga tggttcccct
     7141 gattggtatg agaaggttat tttgtgcagc agcggttctc gtgctcgcat ccctggtgct
     7201 cgcggccggg tgcacgtcgg ccccgtccca ggcggggcat gtgacgatcc ggggcgtgga
     7261 catcagcatt ccggacggtc agccgccgtc gaacgtcacc cgggtcacgg tggtcccgta
     7321 ccttgacgcg gtctatgcag cagtggacgg cgtcgacctg caggtcagcg caaaagaggc
     7381 cgggacggat atcgtcgtcg cggagacggt cctccccatc ggcaacctga cgagcggcag
     7441 gactgcgaaa gaggagattg agctcgcgct cgagaacggc aggcgctacg atatctgggt
     7501 gacggtgtgg caggacgggc gcatgcggga gtcgggctcg gtgaacgtct tcctcccgga
     7561 caggacgaca gccgggcagc ggctggcata ctcgctcctc tccgtctccg cgctcgacgt
     7621 catgaccccg gagatcgacg ccgacccgat cacgctcgat atcgtcaccg ccatcacgaa
     7681 cggcggccag gcctcctccg gggatcttgc gatggaggtc cgggccgtca acctccagac
     7741 cgggatcacg gccgtccgcg agtcccggcc gctcgggacg gtcgccgggg gctcgggcgt
     7801 cgagaagcag gtgagtgtca cggtccccaa cggctacgac tacgagatcc gcatccggct
     7861 catcgagggc ggtgcagcct ttgcgaccag caccggcagg attaccctgg cgccgccgcc
     7921 gcaggtggtc ttcgccggcg gcacggtgct tgactccggc aaggtcacgt cgccggtcgc
     7981 cctcccggtc ccgaccgcga ccccggcgaa gccgtcctcc gcgcaggtcg ggcagttcag
     8041 ggtccagagc agcggtgcgc cggtgccgac gcaggcaccc gggtttgcgg ccgggctcgc
     8101 cgccgccggg ctccttggcg ccggacttgc gagacggagg aggtagggtg atgccggagg
     8161 gcctcaccgc gtggagggcc tacctctacg ccgtctcgtt cgtcgcgctg atcgttgcga
     8221 ttctgggcgc cgtggacctc attgccgtcg cgatcgaggt cttcgtctac ccgccgcccc
     8281 ggccctaccc ggtgccgcag tactaccccg ggctctcggg gagcgtggcg caggtcgtgg
     8341 tggggctcgt ggtctggggc taccactgga tgctgctcaa aaaagagcac tgaaggggat
     8401 tcacctcttt ccgaggtagc tcgcgacaat cagcagcccc gcggccgcgg ccgccgcccc
     8461 gaatcccggg agcggggtgg gcgacgtctc cgccgggagg gaggaggcgt agggcgccgg
     8521 ctgcaccgac gtgtagacgt gaaccgtccc tcccggcggc ggcaggggcg ggagctggtg
     8581 gcgcgacatc gtccctctct ccttattcct gcagacgaac tcttccggag acgggagcgt
     8641 ggtgttcacc gcgacccgta aggtgccgcc ttcgacgacg cccacgaacg tatcgtcgat
     8701 ggagacctcc tcgccatcgg accggtagat caggtaccag ccggtctcgt tcacccccgc
     8761 acccggggag ggctgctcga cctgatagcg ttgcggaaag aggtcgatcg tctccccggc
     8821 ggccggggtt ttggtgatga acccggtata cagcagacgc tctcccctcc agacggtata
     8881 ctcccgtgcg ggcggggccg ccgggtcggc cgccac
//
LOCUS       DAJB01000126            6220 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_141738, whole genome shotgun sequence.
ACCESSION   DAJB01000126 DAJB01000000
VERSION     DAJB01000126.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_141738"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1102 a         2104 c         1688 g         1326 t
ORIGIN      
        1 ggggaattcg gccgggatcg ggccgtgata gttcacctgc gcgggacata acgtttgtga
       61 ttcatcacat attaacatta tgttcctgct tcaggatttg agccggggat tcatcacaca
      121 ttcccggccc gatcgcatca ggcggcccgc attacgcgag ttctgataca ttgtgcgaga
      181 acacatattg aagttccaga catataaaca ttgtcacccg catcagaccc aggccgaaac
      241 ttgagccctc acaacggttg aagggccaca taccagacca cccggaaacg ggctgcacgg
      301 catgatgccc cgacaacaca gccttatacc attgtcgtca atggcatata aacatttccc
      361 tgtattgtcg gatccaagca cagggtttgg acccccatat gtgttgggag tatcgcgcat
      421 tctgcatttg aatgcagaat caggcacgag atacgctccc aacttgacta tagtaggttg
      481 actccggggt atatatacat ttaccccgga cccgcacaga gaaccgacat ccgactgcat
      541 agttgcccgg atatgcctct caacaggcca taatatataa gcaaaatgcc ggacgtattg
      601 ccacgcgcgc aaagcgcaca cggagtcagg aatcctttcc cggccagaat tctgtggaat
      661 actcaccaga acggcagaac cagcacccgc cccgggcccg ccgggccccg gggcggccgg
      721 cagcgccggc gctatccggc gtcattatcc ggaacgtggt cgccgacata gatctcgcca
      781 aagaactcgt cctgccagag ggcgagcttc ccctcaagca tcaggaacag gagcgggata
      841 tagacctcgc ggcgggtccg ccccattgcc ccggccaggt caccgagcgt caggatgtcc
      901 ccgttccggg cagcccgctg gaactcctcc atcacgatcg agaccgcatt ctggtatccc
      961 tcgtcatgcg ctaccgccac cacatctccg gcagtgaggt cgagatccgg ttcgtggaac
     1021 acaggagccc gtctccgctg cttccggcgc tgctccttct ccgccgtctt caactgcttg
     1081 atgagttcat agagcgtgac cggcgggcgt ttccgcagac tcttgcgccc caggcgacgc
     1141 tggatctccc gctccaggcg gcccatcggc tcgagatcgc cggccgactc gaagtcgaac
     1201 ccgagatcgg caaacgactc ctccccctca tccgcaaaag agtcctcagc ctcctcaccg
     1261 tcccatccgt cgagataatc cgacttcaag cgcagcagac aggcagcata aaagagcgtc
     1321 ctccccgaga cccggagatc cagttccttg cgtcggtcga gttcggcgag gaaccgatcc
     1381 gtcacatcca cgatatcgat gttccaggga tcgacttctc cccgctcggc catgcccacc
     1441 aggatctcga caggctcttc atccattgtt ctgcacaccg gtcacgtagg tgctcttgtc
     1501 gggacggatc gtcacgccca cgatgcggtc ggctcgctcg atcatgggct ttcgaagcga
     1561 gacgatgatc gactgcgcac ttcccgagag ttcgttcatc atggaagcaa tccgaccgac
     1621 gttgctcccg tcgaggaaca tatccacctc atcgagcgcg tagaacggcg cgggcatgta
     1681 ctgctggatg gagaagatga acgcgagcgt ggtaagcgac ttctcgcccc cggagagcga
     1741 ggagaggagg tggaccttct tgtcgcgcgg ctgcacggca aacgtcatgc cgccggcaaa
     1801 cgggtcctcc tcgttgtcga ggatcagcct cccgctcccg tcggtcaacc gcgcgaagat
     1861 atcccggaag ttcgtatcga tagcggaaaa agcggtcatg aaggcgtcat acttcatctt
     1921 ctcgtacttc tcgatccgtt cgagaagcat catccgctcc cgcgagagca cctccttctt
     1981 ctcggtcctc tcctcgacgc gggcagcgac ccggtcgcac tcctcgatcg cgagcatatt
     2041 caccgcgccg atctttttca acgcccgctc ggcctccgca atcccggcat cgatggcggc
     2101 gaggtcaaga tcggtctcca cgtcaccggc ctgctcccgg agcgccgaaa gttccgcgag
     2161 cagagtgcgt tctcgctccg cgagggcacc aagctgcatc cgggtgcgtt ccatcgcact
     2221 cgacagttcg agagagtgct ggtccagcac ccggatctcc tcgaggatcc ggtcccgctc
     2281 ttcatgcagg ccgacaagct cgtcggagaa ctccttctgc cgcgcctcca gctgggcgat
     2341 caggcggcgc tggctctcga tctgctcccg ggtagcgatg atctcaccgt cgatctcctg
     2401 gttcttcgtc tccaggcgaa cccgctccgc gccgagttcc tcgatgcggt ttgcaaagtg
     2461 ctgccgctcc cgtttggcat cggtgatgtc ggcgtcctta ttccggagcc ggcgctcgat
     2521 atcctcaacg gacttccggt aactctcata ctgttcggtg agaacgggaa cctcggtgtc
     2581 gttgagtttc ttcttgagat cgtcaacctc tgccgagagc cgcgcgatct cgccggcggt
     2641 ccgctcgaga tcggtttcaa gccgcgcaag ctcctcgcca ccggtcttcg cgctctcgag
     2701 catctcctgg agagtatctc tgagggtctg tctttccccc gcaagcactt cggtgcgctt
     2761 gtggaactcc tccacaagca tgcggtagcg tgcaacctgt tcttcaaccg cactccgctc
     2821 cgcccgcttc tcctccgcaa ccacagcaag gcggccgata gacgcctcga tctcccctgc
     2881 ctccgcctcg aggccggcga gtgttgcacg aacccgcatg atctcatcgt cgaccgccac
     2941 cccgaacccg cggaccttct tcgtctgcgc gccgccggtc atggcgccgc tcctctcgac
     3001 gaagtcccca tccagggtga ccatcttata ccggcccatc agccgccgcg cccggtcgag
     3061 ggtatccacc actaccgtcg cgccgaagac gacgcggaag gcacggtcga acgccgggtc
     3121 gaactccaga aggtcgaccg cgtacccgat gatgcccggg tctgcctcga gcggcgagag
     3181 catcggcggg cgaagtttgt tgagcggcag gaaggtgacc cgcccgagac ggttctcctt
     3241 taagtagcgg atcgcatcgg tggccaccgc atcggtatcg acgatcgccc agcgaagccg
     3301 gcccatggcc gcgatgtcga gcgcggtcgc gtactccggc ggtgcccgcc cgagctgcgc
     3361 aacggtgccg tggacgccct ccatgccgag gacaaagtcc atccccctcc cgccggcatc
     3421 gccctgcgcc tgctgctgcg cttcatagcg catcaggctc tgctcgttct cccggatctc
     3481 cttcttcagc cgttcaagtg ccgaccgccg tgcaaagagc gtactctcgg cttcggagag
     3541 gtcgcgctcg atccggcgct tctgtgcctc gcagtcggcc atgcaggagg agtaatccgc
     3601 gacctgcgcc tgcttgttct ccagttcctc ctcgacctgg tggacgcgag catccaggcg
     3661 ttcccgctcc gacgtccgca tccggctctt ctcgatgaag atatcctgtt cgcggaggat
     3721 cttcgcccgg agttcctttc tgctctcgag atcctgcaat cgggcgaaga gctgatcctt
     3781 tactccctcg acttccttgc tctcgctcgc tatacgcgcc tcgaccgcgg tcatcttctc
     3841 gcgctgcgtc gagagttcca tagccaggtt cgcccggtcg atcgagagat tgcggatctg
     3901 cccgacgcat tcctcgacct tcgcctcggc gcgcctgctg tccatgtaga cccgctggac
     3961 cgtctcgagg ttctcgtccc ggctgacctt cagtcgctcg atggtcttct caccgagttt
     4021 gatcccgcct cgcgcctctt cgagccgtcc gacgagttcg aggtactcgg ggccgctctt
     4081 gcgattgatc tcttcgtcga cggcatgctg ccgcccgtgt gcctcctcga ggcttgcccg
     4141 gagagcgtca acctcgccgg aggtgcgctc gagcgccgcc tgctgatcgt gcatcaggtc
     4201 gtggagggtc ccgatctcct gctccttctg cccgacgagc gccgcacccc ggcactgccc
     4261 gaggtgttcc agctgctcct gccaccgccg gtgctccaca gcctgctcgc gctcgtgctg
     4321 gagcgcatcc agccgcgcca cgagttcgtt taagaggatc tcctcacgct cgatccgttc
     4381 ccggaccacc tcaagctccg agaacgcctg ttcgcgctta tgatcgaact cggcgacacc
     4441 tgctatctcg tcgacgatct tccgtctctc tccgtcgctc atctccatga tgcgggtgat
     4501 atcaccctgc atcacgacgt tgtagccctc aggtttgatc ccgagcttcg cgaggaactc
     4561 gatcacctcg ccctgtttgc agaggcggtt gtttaagtag ttgtaactgt agtatcccgt
     4621 cggcgtccgc ttgatccggc ggcggatcgt cgtgccgtcc gagaacgtga ccgtcacctc
     4681 ggcggtgttc ttcccggaat tgacgttgat gagatcggtc aacttctcgg cccgcagccc
     4741 ccgggcaccc gagagagcca ggacaaagag gatgctgtcg atgatgttgc tcttcccgga
     4801 tccgttcggg cctgagacga cagtgaatcc ttcaaaaaaa gggattttcg tctttctggc
     4861 gaaagacttg aagttgtcga tctcaagctg cgtgatgtac aaggatcagg ctcccttcgc
     4921 tatcgatcga ggtcgaccgt gtcatcctct tcagcatgga tgacatcgtc aaccggacgc
     4981 ttcttctgct cgacgaagag tgtccgttct ggggacttgc cgccttttcc acgggccttc
     5041 gccggggcgc ccgcaaattt agggctggcg acgatatact ccgagggtct cctcggctcc
     5101 ggcttcagtg tcccgtcatt ctgcatgatc agttccatat ccacatcgtc cggaacgggt
     5161 gcgggctggg gagcaggctg gcgcttctct accgggcgca cgggggcccg gggttcgggg
     5221 gcatggacgg gcctggattc ggcggtcgcc cggggctccg actggagcgt ggccccggcc
     5281 ctcctctctt cagccaccgc gggcttcttc cggcgctcct ccccgtcccg ggagagtttc
     5341 atcgccacgg atttgacatc cagaacctct tccatcaggc ccttcaccag ggcctcgagc
     5401 tcccgcacct tccgctccag gttgcgcaga tgctcttcgg ctgcctccag gcgctcttcc
     5461 gtcgcctccg ataccgccgg ctgctcatcc ttgagctccc gcaccttccg ctccaggttg
     5521 cgcagatgct cttcggctgc ctccaggcgc tcatccctcg cccccgatac cggcggctgg
     5581 tcatgaccct caagttcctg catcttccgc tccagattgc gcaggcgttc ttcctctgcc
     5641 tccaggcgct ctcctctcgc ctccggtacc ggcggctgct cacgggcctc aagttcccgc
     5701 accttccgct ccaggtcgcg caggcgctca tcctccacct ccggtaccgg cggctggtcg
     5761 tactccttca ttgtctccat ctccttctcg cgctccgcga gttctcgctc aagaaaccgt
     5821 atcctcgcat ctttctgcgc catgactctc cctcaaatta atccttgata ataattacgt
     5881 aataatatta ttatagattc cccgaatgaa gaattattac attttatccg ggatgcggca
     5941 gggaaataaa cagcagttgc cacagatgcg gccgtcatcg acaaccacgt ataaaagaaa
     6001 gaatattacc tcactaaagt aaccctcttc agcatgtcta gtctggcaaa cttcgatcca
     6061 gaagtcgcgg gtctcatcga gaaagagcgc ctgcggcaga tcaatgggtt ggaattgatt
     6121 gcctccgaga atgtcgtcag caaggcggtt cttgaggcga tgggttctat catgaccaat
     6181 aagtacgccg agggatatcc cggcaagcgc tactacggcg
//
LOCUS       DAJB01000127            9040 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_25059, whole genome shotgun sequence.
ACCESSION   DAJB01000127 DAJB01000000
VERSION     DAJB01000127.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_25059"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    342..419
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1923 a         2509 c         2624 g         1906 t
ORIGIN      
        1 ttaaccgggg gtagttgaca caggttcctg gcgcatgcat acacgatagc ctttgggacg
       61 ttgacacggc ggaggcaggc agcgtccagg ggatcccgtt cccgctggat tcgcagacgg
      121 cggcgaaaca aacgtgaacc acttatctaa tcgccggtgc aagtactcgc gaaagaacat
      181 cggagcattt gcatgagcac accatcacca gactcacccg attcctgcgg catgatcctg
      241 cggcccgtgg ggagcattcg caacgctgta caggaaccct tcctcgtcgc gggcagggat
      301 ggaatctcaa tgcgggaagg gcccgacgcg agcagggatc annnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
      421 tcttctcgac ctgcagtccc gcccgtccca accccgtgct cgcgaccgtc gccagactgc
      481 acgcgcgacg ggggaacgtt ctcgaggtta ccggactcga cgccgtcgac ggcagccccg
      541 tcatcgatat caaaccctac gtcccgaccc agtacccgaa aggcgacgtg cggatccccg
      601 cgtggatgga cgggctcatg aacgaagtgg gcgagcggca ggcgtgaatc cttcctgaag
      661 gaagaaggga aaaaggaata ttctatcgct tttttgcgac cgatcgttcc agcgcgggca
      721 gtttgtcctc gaacgccacc ccgtatttcc tcgcgaggat gaccaccgcc gcctcgaccc
      781 ggaggttgcc atcgaactgg tcggtgacga cccggtggag ctcggcgacg acctcctgcg
      841 gcggcttccc ggttgcggcg gtgatccgcc cgatcaactc ttcgtagggg ctctccgccg
      901 ccgcgaggac atcggaggtg ggcttgaacc cgagtggtat cgagacctcc gcgacgtcga
      961 agagcggccg gatggctccc ccgtctacct cgatgaggcc ctccgacctc gcccggtcca
     1021 ggagctggtt cgcctgctcc ttattcatcc acttccggtc gatggcgatg tagaagacga
     1081 actcgctcct ctgcagccgt tccttgtgca tgtgtttgaa cggtgcggca accgcaatct
     1141 tgacgctcac tcgcaggcac ccttgagcat tcttcgcagg tccatcgcat ccagatcccc
     1201 gatccggctc tccttggcga cgtagcactc cgtcgccggc cctttgtcga cgatacggag
     1261 aaaaaagacg ttctccgaga ccggcagccg tttatccggg gtaagaagcg tcctctgcag
     1321 atcgacccgg aagtccgcca cctcggtgac tttctcgggg agcggtgcca gggcagcgag
     1381 gtccaggaac tgggtccgcc cgtccgcgac ctggatcagg gtccgctcct tgacgaggcc
     1441 gtcggccccc cgaacggtcg tatgggtgtc gtgcatcctg actatgcagg caaggacctc
     1501 ccggaacggc cggacgagtt cgaaatcgag gtcgatggat tgggggaaac gatggtatat
     1561 cctgcgcatc actagaattg aggtctgcgg aacgtaaaaa ggatgtgatc cgctggagac
     1621 acaaaagttt ttggcacacc tctccctccc tgcacctgta tgacggacca tcttacactc
     1681 ctgcagatga acgactcgca cgggtatctg gaaccgcacc aggaactctt ctatgccggc
     1741 ggcgggcccg ggtaccggac ggcggggggg tatgcccgga taagtgcaat ctttgaggag
     1801 atccgcgatg cgcagccgaa tagggtgctc gcatttgact gcggcgacac catccacggg
     1861 acctaccctg ccgtccagtc ggaaggcgag gcgctcgttc cggtcttaaa cgccctcggg
     1921 ttcgatgcca tgaccgccca ctgggagttc gcctacggcc cggagcagtt ccggaaggtg
     1981 gccgggaggc tcgactaccc ggtccttgcc gtcaactgct acgacgatgc gaccggaaac
     2041 ctcgtctttc ctccctatac ggtctgtgag accgaggggc tgcaggtggg cgtcatcggc
     2101 atcgccgcca cgatcgtcga caaggtgatg ccgaagtcat tctcgacggg actcaggttc
     2161 tctctcggaa acgccgaact cccgggacag atcgctcacc ttcgcgacga cgagggggtg
     2221 gatctcgtcg tggtgatatc gcacctcggc tttccccagg aggtgaaact cgcccgggag
     2281 gtggacggga tcgacgtcct cctctcgggg cacacccaca accggctttt cgaacctgcg
     2341 gtcgtcaacg acaccgtcat catccagtcc ggctgccacg gctccttcat cgggcggctc
     2401 gaccttacgg tcgagaaccg gcgggtgaaa cacttcgacc acgacctcat cgtcgtcggc
     2461 gaggagatcc ggcccgaccc gggggtcgag gagatggtcg ggaaggtcat ggacccgcac
     2521 cgcgagcgtc tctcccgggt cgtcggggag acccggacag acctcaaccg gaacacggtc
     2581 ctcgaggcca cgatggacaa cttcctcctg caggcgctca tcgacgccac gggcgcccag
     2641 atggccttct cgaacggctg gcgctacggc gctccggtgc cgccgggccc cctcacggta
     2701 aacgacctct ggaacatcat cccggtcaac cccccggtct cgacggtcga gattacgggc
     2761 cgggaactcc gggcgatgat ggaggagaac ctggagcgga cctttgcacg gaacccctac
     2821 gaccagatgg gcggctacgt gaagcgctgt atgggcgtca ccctctactg caagatcgag
     2881 aacccgcccg gcctgcggat ccaggagttc tttgtggggg ggagaaggct cgatccggac
     2941 gccgtctacc atgcggcgtt cgtcaccggc cagggcgtgc cgccggccta cggtaagaac
     3001 cgggagaacc tcgatatccg ggcggtcgag gcgcttgaac gctacctttc gagagggcct
     3061 gtcagcgctg acctccgcgg gagcgtgacg gcgatatgat gcccactctg cgggtcgtca
     3121 tccacctgga tgagcgcgag aaggcggcac tggcgctcag gaacgcgaat aacctcgtca
     3181 cggatcttga gggggtcgag atcgaggtgg tgacccatgc cgacggggtg gaggggctcc
     3241 gtgccggcgg cccgaacacc gacatcatgg acctgctcgt gggccagggg gtccggttcc
     3301 ttgtctgcga gcaggcgctc cgctcccgga attcaccccc ggagatcttc cccgactacg
     3361 tgtcgaccgt cccgtcgggc atcgtggagc tggttgtcag gcaggcggag ggctggtgct
     3421 acctccggcc gtaaaaaaga agggtatgtt atgcgagcag aacagcgttg acgacgccgt
     3481 cctggcccgg tctgctgacg atccgtgcgc gcccgatatc ggtcttgatg atagcgcccc
     3541 tggtcaggag gttccgcctg acgtagttgg ggttggcgct gtttgcctcg accgtctcga
     3601 tcttcgcctt gtgggtctcg ccggttgcgg ggttcgcgac cgtcgcgtaa tctaccctga
     3661 gtgcccggac cttctggttg cccccaaagg tgcggacaat cttcttgcgt tcttcaccga
     3721 tgtgcgtctc tgccggagct cttccgatct ccgacctctt cttgccctgg gaggtgtggt
     3781 agcgtccacc cgatggcttc cttactgatc ttccttgcca ctgcatatga ccaccgaaaa
     3841 tactctgttg aggacgcgat acccgtcgtg ggatagcaat ctctcgttaa agtgctataa
     3901 ctattcgaga ccgggatatt taaccctgct gatcaggcga ggatctcgtc cagcagatcc
     3961 tctagcccct ctccgatctt catgttcgtc cggaagacct gcatccccgg gttgtagcgg
     4021 tgcatatcct gctccatccg gtcgaggttg gcgccgacga gtggagcgag gtcgatcttg
     4081 ttgatgacgc cgatattgct gctgcgaaac atcatcgggt gcttgttcac cacatcgtcg
     4141 ccttccgtcg aactgacgat gacgatccgt ttctcggcgc ccagccggaa atcggtcggg
     4201 cagaccatgt tgccgacgtt ctcgatgaag aggatgtcga tctcatcgag cggcaggtgt
     4261 tcgatggcat gctccaccag gtgagcgtcg aggtggcact ccttcccggt gttcgcgttg
     4321 taggccggga cgttgagggc gacgatccgc ttgaaatcgt cgtcgccata gacatccccg
     4381 gcgatggcgc cggcgcggag tccccgatcc tgaagcagcg gcacaatgcg ctcgatcagg
     4441 gcggtcttcc cggacccgat ggcgccgagg aggtcgaatg cccggatgcc gtggtccttg
     4501 aggaggtttg cgttggcgtc tgcgatgcgg ttgttgacgt cgtagatgtc cttctccacg
     4561 tggacgtcga tatggtgcat ggtatatact gtgatcagga tctgtttata ggttaaccac
     4621 ccaaaccata cgacaatgag cgttgagcgc atcctgtatc cctgttattt tgacgtcacg
     4681 ctacagcggc gggaggggcg tcgcgtcccg aggaaccttg gcgtgaagtc gccggaactc
     4741 actgccatcg aggctgtcct gcggaagctg aaggtcccgt accgggtgga agagcaccac
     4801 caccccgccc ggtgggcgga gcgccaaggg cggatcgtgg cggaatggga aggaagcaag
     4861 gaagacctga tccggaaggt tgccggcagt cttgccggcc ggaagtgacg gtgatgtacg
     4921 acctgcacac ccacaccatc ctctccgacg gcgaactcct cccgacggag ctggtccgtc
     4981 gggctgccgt gctcggttac gacaccctcg ccgtcaccga tcacgcggac gcctccaacc
     5041 tcgcgcacct ggtggaggcg gtcgctgagg tccgggattc ggcgcggtgt tacgatgtgg
     5101 gcctgcttgt cggggtggaa ctcacccatg ttccgccgtc cctgatcccg gcatttgcgc
     5161 gcgacgcaaa acggctcggt gccgatatcg ttgtggtcca cggcgagacg gtgatggagc
     5221 cggtcgctcc cggaaccaat cgcgccgcct gcacgtgcga gtacgtggac gtgctcgccc
     5281 atccggggct catagcagtc gaggatgccc gcgcagccgc ggagcgcggg attgcgctcg
     5341 agatcacctc ccgggggggg cataaccgca ccaacggcca tgtggtacgg gtggcgcggg
     5401 aggccggctg tcagcttgtg gtcgattccg atacgcatgc gccgtctgat ctgatgtcta
     5461 agggggcacg gcgggcggtc gcgctcggcg ccgggctgac agaggcggaa tcccgggaaa
     5521 ttctctctct agatataaat cggcttctgc agaagtgaga tggggtattt atattttagc
     5581 aaaacgtttt tatactctaa tggccaatat atatgtgtta ctaaatactt ccaactatag
     5641 tatcccctag aggttgcagt ttgcggttaa tcggacgttc tgtaagtgct tgcggcaatc
     5701 gtttgttaat cttgcgatgt gatgccgcac agttgccccg cctctacggt gaggccgtgg
     5761 atcgccggtt aaatctcgta gggaaggtag ttgacatctt tggaaatata tcgtctccct
     5821 atgccgtagt cctctgctac aatggctgtt ccgtgcaggt tggcgagaag atctttgcaa
     5881 aataggtgaa taatcgatgg cagaagtaga aaaattaaag cagctgcagt tgcagcgtga
     5941 ggcccttaag aagagagggg agcagaaggt caaggagacg gagaagaagc gcaccgagga
     6001 gagtgttcag tccgtctgtc ccgagtgcgg cagccgccag ctcgtccacg actacgagcg
     6061 tgccgaactc gtctgccaga actgcggtct cgtccttgac gaggagttca tcgaccgcgg
     6121 ccccgagtgg cgtgcgttcg accacgacca gcgcatgaag cgctcccgtg tcggtgcacc
     6181 gatgacgttc acgatccacg acaagggtct ctcgacgatg atcgactgga ggaaccgtga
     6241 ctcctacggc cgtgcgatct cgagcaagaa ccgcgcccag ctctaccggc ttcggaagtg
     6301 gcagcggcgt atccgggtct cgaacgcgac cgagcggaac ctggcgttcg cgctctcgga
     6361 actggaccgg atggcgtctg cgcttggcct gccccgaaac gtgcgcgaga ccgcggcagt
     6421 ggtctaccgc gacgctgtgg acaagaacct gatccgcggc cggagtatcg agggtgtcgc
     6481 ggcggcggcg ctgtatgcgg catgccgcca gtgcagcgtg ccccggacgc tcgatgagat
     6541 cgcagaggta tcccgggtat cgaggaaaga gatcgggcgc acctaccggt tcatctcccg
     6601 cgagctcgga ttaaagctcc tgccgacgtc cccgatcgat tacgtaccgc gcttctgctc
     6661 cggcctgaac ctgaagggtg aggtccagag ccgtgcggtc gagatcctcc ggcaggccgg
     6721 cgagcgcgaa cttacgagcg gcagaggccc gacgggcgtt gctgcggccg ctatttacat
     6781 ctcctcgatc ctcggcggag agcggcgcac ccagcgcgag gtcgccgagg ttgccggcgt
     6841 aaccgaggtc acgatcagga acagatataa ggaactggca gaaaaactag acatcgagat
     6901 catactctga tctcctatct ttgggctcgt agatcagggg tagatcgcta cgttcgcaac
     6961 gtagaggccg cgggttcaaa tcccgccggg tccactgttc ttctttcttt tcggaaatta
     7021 agatcatatt gtgcggcgtt actgttctga gttcagaaac agtgttatct agtaatttac
     7081 ccacgttcag ggcagtattt ttcagatact cgtgcgtttc aggcgatacc gtagtgtgca
     7141 ctcgtttcct aatccgatgg attacagttg gtttcgtctt tcctcctgtt ttcatttctt
     7201 ttttcctcct gtccggacat ccttgggatg gcttagtctc tgcttaggct gccttaaaga
     7261 ggtggtaacc aaaaaggggg tgcagaggcc gcgcacccac gtagggggct gaagatggct
     7321 gaggcctctt tcccccccgc agcgtgggac gtcgcctcta ggtctaacct acattgtcct
     7381 agtgaggtcg tgcgcccact tcttcagagt tcaatggata ctatcaggac tcaatatgaa
     7441 tagtttctca tccgattgag acaaaattga cgaccacgat ttagatggaa gtccgtattc
     7501 cgggcaaatt cgtcccactc ccaccgacct tcttgagtct caactgctcc agcgaaatag
     7561 ctggttatat cctcgctttt caagcccttc tcgaacagaa gcaatacatc acgagtctca
     7621 ctatcagcaa gtgcctgtaa ctccatagct gccgacaaat tcacattatg ctcctcaggt
     7681 ccttcgataa aaaagaaaat tatatggata tggctagatt cacgcaacct agtgcttaca
     7741 ctgcagtttt tttgatgttg ggatacttct ggagcatcaa ctttgggttg gtcaagatcg
     7801 aatgacattc gtgcattata tctttcagaa attaagaaat cacggaagtt tatgagaatt
     7861 tttatattat ctttcttaaa tgagccataa ataccaatgt ttacggctct ttttaattgc
     7921 tcgtagtatg ctgtttttga agaaatctcc ttaaagaagt ccacaaaggt actccgtata
     7981 taagattaaa agagttacgg ccattgaggt cgcatatttg tgacagttgt gcaacatata
     8041 agtataaata agtcatagat ctataacttc acttggtgac gatatggatc gcgcaaagat
     8101 cgaggaatat ctagccgaag tccctgcaga aatccgaagt gcagtgaaac cgctagataa
     8161 cgataaagct tgggcaatct atattctcct actaaaacga cagcaaatgc gttttagtga
     8221 aatcaagagc gaatttaatg ttaaatcatc gggagacatt gacaggtact tgaagactct
     8281 tgtaaatgca ggtctagttt caaaagaagc tagaaaattg agcgaactgg gaaacagtga
     8341 gaaagcctat tactccccga ctaatttagg taagtctctg ctgcacggac tattccagaa
     8401 catactacca caagcaagat cagcaccaca ggatcggaat ttttggacag aagtactaaa
     8461 cagggagtat gtggcatttt atcccggtga aactgaggat cgagaatcaa acctcttgta
     8521 tcgagaatcc tctatggggt acaaatcaaa gagaattact gaacggaaac aattggacta
     8581 gtcaacggag atgaaaaaga tgaagtctga aaaaaaggtt gaagcaaaaa agaatcgggt
     8641 agttgtcgag aagttagggc agaaagtgcc actcaataaa tactacgagt attcaccgtc
     8701 tgagcctaag ttaaaccttg catatacaaa atatgtgaat gcgtttacag gaaatgtctc
     8761 aaacgaggat gtttttatcg actttataga gttacctgga cgcaaagtgg gcggagtaca
     8821 gacaatagac gggtgccgcc tctatttcac acacaatgct gcaaaaacat tgtgcgacct
     8881 tctccatgag atagtcaaac aaattgaatc aaatagtgat gagaaggaca atcaaggaaa
     8941 tgaaccggag gaaaaccagg gaagtgaaag tgaggagggt ggaaaaaata acgaataatg
     9001 gtcactaatg attacccgac attccgcacc aattttttca
//
LOCUS       DAJB01000128            2984 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_111792, whole genome shotgun sequence.
ACCESSION   DAJB01000128 DAJB01000000
VERSION     DAJB01000128.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_111792"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          450 a          986 c          997 g          551 t
ORIGIN      
        1 gctcccggtg gggaggggga tgaagcagga agccccctgc gtaagctggg ggtagttcac
       61 aacgcggcat cgtttgcatc catctacctc ttcagcctgt actttcagta tgtccgggga
      121 ttcgatgcac gggtggccgg gggtatcctc ctcgtcgcaa gcctgatcac ggcgtccctg
      181 gttggatacg ccggccggct ctcggatcgg ggaagcccgc acctggtcgc agcggccggt
      241 gtcgcgatga cggtcgtggg cctcgcaatt ctctcgttca tcggcgaggc catgcccctg
      301 gccgtcgccg ttcttgcgct ggtgctcctc gtggcgggag tggcgttctt ccagcctccg
      361 gtctacagca tcgttatcgg agcggtcgaa agagcgatgt atggggtggc cgcaggactt
      421 gtggaaacga tgcgcctcct cggcatgacc gtcagcatgg ccgtcaccat catcattttc
      481 gctctcttct tcgggagcat ggagatcatc ggcgagaaca tcccccggtt tctctggagc
      541 atgcaggtgc tcttccgcat ctacttcgct ctcgccgtgg tgagccttgc ggtgacctgg
      601 ctcgcggggc gcgctcctcc tcgcgcgccg tagccccggc gcaaaaagga tcccctgcct
      661 acggttcgag gaccgcacgc gcccggtcgg ctatcagcag cacctcccgg agggggatgc
      721 ccgcgtcgtg ggcaatggcc gcggcgtcgc cgtaagcgac ctgctttgcc gtgaggaccc
      781 ccgcggcagc aagcctggcg gagaccgacg ggctcacccc ggtaacgatg gtgaccgggt
      841 aggactgagt ctcctcgatc atcgtccgga ggttctgctc ttccgggtag tcccacccga
      901 tatgcccgat cccccggcag gtcgcgtagc gcttcgcgtg agcggagagt ttggtgttgc
      961 agacgatcag ggcgccgtcg atggagacgg tgcactgccc ggagcggtac ccctcctgga
     1021 ggtcctcgac gatcgcccgg gcgatccgcc cttcgtcgag gccggtcatc cggtggtggc
     1081 tcgcgtggtg tttcacctcg acaaagatag tggtcccgtc tttgtaggcg acggcgtcca
     1141 cctcgtgctc cccgcaccgt ccggcaagga tgcacccgct ctccacccgg taaccgtgct
     1201 cccggaggag gacccgcacg aactcctcga actccggctt cgggcggagg agggcgagcg
     1261 ctctccggag attggtccgg tgcgcgacgg cggggcggac aacctgtgcc cgggcgcgga
     1321 tcatccggag gaccgcggcg gtcttcacac cgtccggaac cgactcctcg atctcgcggg
     1381 cgacccgttc ggcatccgtc tcacccaggc ccaggttccg gagcgtccgc cgcaccttcc
     1441 tccagtcgaa cggctgcggg ctgccgtcgg ccttcgtgac gtacttcatc aggtacggta
     1501 ggtcggcccc gaagacgaaa agcgttcgct accgcgttct ccggcggcac atccggggcg
     1561 gagcaggatg ggtgagagga tcggaggcag ggtatgccta cccctgccat gttgagatgc
     1621 tgccttgcag ctccggcccg gtcgggtctg cgccggggcg ggtgcccatc gggggaggcg
     1681 agtataaacg ttgcggttgg gcgtgtcctt atcttctttt acgcccaaca gggaccccgg
     1741 gagaatggaa gcgttacaac gcccggtcat cctgatcctc ggcgcggggg cggtcggtct
     1801 ctcgctcgcc ggaaaacttc gtggggtggc gagggtctat gcagcctgcc gaccggtgca
     1861 tgccgacgcg atccgggagc ggggcctcgt gatggagggc gcctggggag acggcactgt
     1921 cgacgccatc gcctgtgtcg cgggtccgga ggaggccccc tccgggatcg atttcgtcat
     1981 catcaccgcg aagggcaccg atacccgggc gatctgcgag gagtacgccg gggtgatccg
     2041 gggccggccg gtggcaagcc tccagaatgg tatcgggaac gaggaggtca tcgcctgcta
     2101 caccgatacc gtcatcggcg ggaccgtgac gacgaacttc tcggtcttgg gcccgggcca
     2161 tgtccgggtc ttaagcgaga gcgccccgat gcatctcggg atctggtccg gcaacgacgc
     2221 cgggatcctc gaccggttga tcggtgtcat ccggtccgcc ggcatcccgg tcgaggcgga
     2281 caccgatatc cgttccggga agtggaccaa ggcactcctc aacatcgcgg tgaacccgat
     2341 ctgcgccctt ctgcgggccc cggtcagggt cgccgccgac ggaaacgtcc gggagaccgt
     2401 catcgggctc atcaacgaaa cgttcgccgt cgcggacgcc gaaggggtga aactcctctg
     2461 ggcgaccgcc gacgactatc tcgcccacct cttctcagtg caggtgcccg acttcgccga
     2521 ggtctatccc tccatgtact acgacctccg gcagggccgc cggacggaga tcgacctcct
     2581 caacgggtat atcgcacgcc tcggggagcg ccacggcatc gagacgccgc acaaccggtg
     2641 catcgccggc ctcgtccgct acgcggaggc gcacccggag aaatcgtgac gcttcactcc
     2701 ccgcccatct tcgcggcgag cagtcgggcc gactcttttg ccttcgcttc ggtcctccag
     2761 agcccggtcc tgccgatggg gttcccctcc cggtcgtaga gctgtacgta gtagccgccg
     2821 tcctggcggg cgaaccggac ctcgccgacg atgtcggggc cgtactcctg catgccctga
     2881 tcttgtcggc gcgaaggata tgatagttcc gcgtcccccg gcccggttct gccggctgta
     2941 tataggagaa gtgctctacc tctgcggggt tgaaggggca gagc
//
LOCUS       DAJB01000129            3568 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_58824, whole genome shotgun sequence.
ACCESSION   DAJB01000129 DAJB01000000
VERSION     DAJB01000129.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_58824"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          849 a          913 c         1046 g          760 t
ORIGIN      
        1 ttataacaga acgttgattt ctcacatgtg gataccggga ccggtatccc gatgcgcggg
       61 ggtccacccg gccaggagcg aatcggatgg gtgaacatcc cgcatgcccg gaggcaaaag
      121 agtgtacgtg ccttgtgttc ttgaaagtat gcctcacttg cctccggaaa attatcggag
      181 gaaagcaaat gaatggaaag attggaacgc aggcattctc cgtgctcctc gccgtgctgc
      241 tggtgagcgt gggaattgtg ccggctgtga gtgctcagga tgcaacagga acccctaccg
      301 ccgcaggcag cggcgagcca gccttggtca tcggtgattc tatggatgtg cttattgcgg
      361 gtgctacatc ggtatccgat atgcgagcat ccggaatcac cgcgccggaa gaactgaata
      421 tcccaaaggg cattgcccgg tacgatctcg tgacgttcga ccatgctgca ctgaacaaac
      481 aggtccgcgg ggtactccct ctccagatcc acggcaagga ttaccaggcc gaactgcaca
      541 ggatggattt cgagcagatc gacgacggta ttgattccta tgaaggaacg atcgccggag
      601 ttgacggaag cgatgttctt ctgacgaccg gggaaaacgt cctcgtcggg agtgtaacac
      661 tcgggaatga gacattctgg atcaggccaa ttgagccacg cgcgcgtgta gaacagccag
      721 aaacatcgtt gcacatcata tacagctcag aggatatact gtatccacaa gaacctgctc
      781 aagtggatca gggaccggtt acaccaccgg aaggttatcc cacttggcag atggtaggag
      841 agcctgaaat cggcgttatg ctgactgctg aacagtatgc aacaatcaac ctgctggtgg
      901 ttactgacaa ccagttctac acgaacgaaa gagactggaa agcgtctgct caggaaatag
      961 ttgctgaagc gaacagacaa tacgacagag acgacatccg tgttgcgctc tgtgtcatgg
     1021 catatgacga ttcgaggcgg aatcaacttt cgaataatgc ttatgttctg actgatcctc
     1081 tgcatgcttt ctggcaagtg tacccagact cggatctcga tctatggtcc tcagatctcg
     1141 gattatactt gggaggttac gacaaaacta cggcgtatga tcagggggct gcatatggat
     1201 tcacaaacag aggaagacat gcatgggcgc agatggtgcc agatgacctg ttttatcagg
     1261 gtaccgatca cggccgcaaa tatgtagcca ttcatgaaat cggacacaat atcgacgcca
     1321 accacgaggg cacaaattcg agtatctata accaggcata cgattggtgg gatctcgggc
     1381 tctaccatcg gacggtcatg tggagcggtt atctggagca gtttacactg ctggagttct
     1441 catccgacga ctatcatgga gatgccacgc atgacaacgc acggagcatc cgcgaagcga
     1501 agtggcaggt tgccggttat gtgtgaagaa aacccaaccc atttttcctg ctctctccca
     1561 aggaataaaa tcgatggcgc tgttgtatca tataacaaac atgaggttca ggcggtattt
     1621 catcctcacg aggatgcttg ggatccacgc gtgtgcaggg attaaaaaca gagcgtcctc
     1681 ctctgtagag atgaatgaga ctccccgatg cgggaatagg gctatccagt tctcacaggg
     1741 tcaaatgaag aaaacgagag tgctgtatgg gacctaacat tgacagacgt ttgttcgtgg
     1801 tcggggttct ggcattcatt ggcgccacca tcctcgttgc gacgattgta ctcatgtgca
     1861 cggccatttt tacctgggtc gaaacaagta gcggcttccc agtgttgtac gtctcagagg
     1921 tcagggggga gcacctgcag aatgcatcca ttatccacct gaccgagaag gacttcgagc
     1981 agtacccggc actcgactcc gtgatccggg gcgataacag gggtcccgat ccgtggaagt
     2041 tggagtaccc ctccgacgac cctgacgaac gtgggatagg gagtgttgcg gtgacgtatg
     2101 tggaacgtga cgtgctcatc gagtcgtccg gcatagacct tgagacgagg aaacgaccat
     2161 acctcgagta caaaggcgcg tattactata ccctcgtctc gattccctga cacggttaga
     2221 atgggtgcaa ttggaacgga taaatataag gaaccagaat ccaggaacct gctcaaaatg
     2281 gcgggggttg gcgtcgtagc atctgggatg aaagcataga ggagattaca tgaaatcaaa
     2341 accagacaga cgtttgatca tcgtaggggt tctggcgctc atcggtgtca tcctcctcgt
     2401 cgcgacggtt gtgctcacgt gcaccgcccc cttcgcctgg ctcgaagcaa gcggcgggtc
     2461 tccgagattg gacgtctggg aaattagggg tgaactgccg ccggaaaatg catcgatcat
     2521 ccacctgacc gagaaagact tcaagcagca cccggctctc gacgccgcaa tccggggcga
     2581 caaccggggt cccgggccct ggtattcggg agacacaccg tacggtgtcc tcgacgagcg
     2641 aatgattggg ggtgctccgg tgacatatct ggaacgggaa gtacttatcg agtcatttgg
     2701 tcccgatgtc gaggcgagaa atcagccgta catcgaatac gacggtgcgt attactactt
     2761 cctcatcctg atcccctagg atcgggtcaa aaacaggtgc tattggaatg gaagaagata
     2821 agaatcctaa atcaaggaac cggttcaaaa cggcagggct catcgtagcc tgcggcctcg
     2881 ccggcatcgt cgcggtcgtc ctcggcttca tgctcgccga tgccctcatc gtctacgctc
     2941 acgagagttc gggtgggcaa ccgatgctct acgtcatgga gagggcggag ccgggagaga
     3001 gcacgatcgt ttccctgacc gagaaggact tcgaacagca ccccgcactc gacgcagtcg
     3061 tccggggcga ggaaaggaac ccctcggcgt gggagtgggg ctaccgggat caacgtgtca
     3121 tcggggggac gaacgtctcg tacgcggaaa gcaaagcgct ccacgatact tacggtcccg
     3181 gaggggagca tgggctgtat tcgctcctgg agtacgaggg tgcgtactac gaggtgctga
     3241 cgacgtggcc gtgaactggg cacgtcgtgc ggcggtacta cctgccctga tgtgggattg
     3301 cccccggtgc cagggtgctt gccctccgtc gttcggcgcc ttctcggctc tgggattgca
     3361 atggtcacat ctgggtgcga attcagtctc gcgggtccga cgcagagatg cgtgtcaccg
     3421 agaccgttta cagttgaacc cgggttttga tccggagtcc gggcgttcag cgcgtctcca
     3481 gcctgatcgt gtgctactgc taggaggatg catctggagg aatttcctat cagtaggagg
     3541 gacccctggt atcggacccc tctctgcc
//
LOCUS       DAJB01000130            7952 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_80763, whole genome shotgun sequence.
ACCESSION   DAJB01000130 DAJB01000000
VERSION     DAJB01000130.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7952)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7952)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7952
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_80763"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1628 a         2287 c         2405 g         1632 t
ORIGIN      
        1 cgtacctcga atacgacggt gcgtactact acctccttgt ctcgatcccc tgatgagttc
       61 aaaagggtga aatcggaatg gaagaagcta cgaaacctaa atccgggaat cggttcaaaa
      121 cggcagggct cgttctcgcc tgcggcctaa tcggcatcgt cgcggtcgcg ctcggcttca
      181 tgcttgccga cgccgtcatc ctctacgcga tggagagttc gggtgggcag ccgatgctct
      241 acgtcatgga gaggatggag ccgggggaga acgtggtcat ctccctgacc gagaaggact
      301 ttgccgacca tccggcgctc gatgcggtca tccggggcga agaatgcaat ccatcggcgt
      361 gggagtgggg ctccggcgac cggcgcttca ttgggggtgc tcaggtctct tacgcggaga
      421 gcagggcgct ccaggatgcc tacggccccg accgggagac gtgggtgtac ccgcacctgg
      481 agtacgaagg ggcgtactac gtggttgtga cgacgtggcc gtgaaagggg tcttaaattg
      541 tcggatagta ggtgcgctgg aaggctgcag gtacgtcgaa atgatgcaag gtttgtaccg
      601 gctgaaactc cctactacaa attagcggtg atcgaatgaa gaccgaatgt ttcctttttt
      661 tcgttctcat gatcctgctg gcgtcctgcg gttgtatcaa tacccgggac gccgtcgggg
      721 atgacggcat gcagaacggc accgtggaag gattgccgat gaacggctct tttacacccg
      781 cctccgcggc taccgggggt atggggatac ctgtcgacct ctctcaggtc gagatcaggg
      841 gcggaccaaa cttcagcccg aatgttgcgt taatctcctc gatccttcaa aacgatccgc
      901 gtacagggct cctgatcgag aatgggtgga acatcacatc gatcaatgag ccgttacagt
      961 cctggggcat cgtggacccg gtacggctcg aaaggtggaa cgtcacatcc atcaacgaga
     1021 tgaacgataa aaaagaccga gcccggggct tcgctgccgt agagttccag aaggaggggc
     1081 tgtcgttcta catcggagtc gatgaacagg aggggcgtac cgccgggggt tactgtggtg
     1141 ccgaggtctg gattgcagag cccgtttcag gtcctcgtcc cggggactac tatcaggcca
     1201 gggactggac ggagggatgg tggcacgtct tcgatgaccg gaatgagagg gtcgttatga
     1261 tctataacag cacgacgttc ctctacctct atccgtcgta cgcgatcatc agcatggagg
     1321 ggatgcttga ttgatctcac ctgtgaagtg tcgtccttcg ccggggatgt tgtgggagtg
     1381 cgtgaacgga actgtttggt gtggagaaac tccgcggggt ccgggctgca caccgacggt
     1441 caggagggtt ggaagacgat gaaaaccgca cccgtcgttt ttctgctgtt tctttctgtc
     1501 tgcatcgtgg gctgctgcac cgagggggcg gtcgacattg caggcccaaa cggcgacata
     1561 caggggaccc tggtgcaaca gcgccctgga atcaacgtat cagatgtccc ggtaaccgcc
     1621 ccggaattga tccggaccaa cggctcgtac atcgccggca ttgcgctcgg cgacgagcgg
     1681 gcacgggctc tcatccgaca cggcggcaaa cccgaatcgg ttgccgtgct cttccactcg
     1741 tgcccgcgaa acgaccccga ttgcaaccgg aacccgagcc tcgtcatccg ttacggggag
     1801 atccggtttg ccgtcgccgt cgacgaggcg gccgggacgg tgctcggcgg ttctgcgctc
     1861 gtgccgaaca ccccggaccc gaacaagccc tccccgacgt attataaggc ccgcgatctc
     1921 tcgaccggga ccgagacagt ctacctgggg gatgccccga tcatgaccta caacgggaca
     1981 tcgctcctgt acctgaacga gtcgtacggc tgctctgccg gactggaaga gggtgccgcc
     2041 ccggcagaag acctctccga cctcgacgag atcgtggcgc ggtatcggtc ggcgcacccg
     2101 tccggggaga tcggcaccgg tcctgctccc gaaccgtgtg gggactattc gaagaccggg
     2161 gagttcgtcg aggtcgtcgg cgggttcttc gacctcgtcc gggaggaccc cgccgccggc
     2221 cggctcctct ccgacggggg cgagatcgtg ggcgtcgttg tcgcctgccc gccgtccgcc
     2281 gcgtccgaac ccatgggtcc ggggtgctac cctgcggtcc gcgtcaggca cggggagatg
     2341 acgatcgact acctcgtgaa catcgactgc atgcgcatcg agagggcttc cgtcgaggtt
     2401 ccgtccggat atctctcccg tacggtcgat aatgtaactt atgtcgtcca taacggcgag
     2461 gtggtgctcg tcctatgagg gcgggcgcgc ccctggtttg cgtgcggagt cgctctcgcg
     2521 cggcccgacg tccggatgtg tatcaccgag atcgtttaca gttgaaatcc gaatccttcc
     2581 ctatcggcat gcatgacccc ctgatatcga attacccacc gtcgcgcagg ttctccccgg
     2641 ccggctgcgg catgcttcct ggagcaaaac ggatcagaat gccttaacgt gtagagagga
     2701 tcgacagaaa tcgtttataa aagaacgttg atttctcacg cgtggatacc ggttctggta
     2761 tcccgaagcg cggggggttc acccggccca gtagcgaatt ggatgagtga acgtcccgca
     2821 tgcccggagg cgaagggatg tacgcattct ccatacttga aggtatgccc ctcctgcctt
     2881 cgggaggagg aaagcgaaat gaacggaaaa attggaatgt gggcattctc catgctcctc
     2941 gccgggttgc tggtgagtgt ggttgtggta tcgatagcaa gtgcgcaaac aagtatagat
     3001 gcgatcgctg aggaagaact tgcggatagc gtaatcatca ctcccgactt cagttctcag
     3061 gacattgtcg tttcaccatc gttaggggaa gacgacctga taacactggc gtttccggaa
     3121 tcctggatca aggatcggaa ttcagcggat tattccgatc gggttgaact tgctgatgca
     3181 cacgtgttac tgaaaaatga gtgttctgat gaaaagactg gattaagata cttctctccc
     3241 gttcaagtaa ctgaagccca gagtctgagt gtgttaagga ttccgaaaaa aatgtttgaa
     3301 ctttctcttg ctatgaacga cgggagcatc tcatttccta tgaagtattt taccgcgtac
     3361 ccggacatgc agaccatgct gagcgaagtg cgcgtagcca ccccctctga gcccgaagtc
     3421 cactcacctg aaaatgcgcg atccgcttcg tatgtatccc cacccctgca cggtgaatgg
     3481 gcacaataca atgtgaactc ccagtacgct ggcaggccgg ttcacctcga ggggctgatc
     3541 aagccgggtt cgtttacaaa taacgggcat gaaggcgcaa tctatcatga gcgtgagatt
     3601 tatctggatg gaggagatgc gattgagtac atattctatt acgacgagga ttattatggc
     3661 gacaaaatct ggctgggcgc agcgatttac gataacagtg actcattcca gggatgtccg
     3721 acaattaagt ggttcgatgc aacctcaaga cattggtacg attacgattt cacgataagt
     3781 agcgctggaa cctactacat ctggttccgt gattgcacga cgggcagctg gaaggagcac
     3841 atttactacg acaacgacga cccctccgct tcgatcaatc ggatctgtgg ttccgctgag
     3901 atctatgccg atgtgcctgt tcagtattca ttcgaggcga taacggatcg catgatcgat
     3961 gaatacgtga gaacaaatga cggactaacg aaacttcccg gagaggtttt ctcctgggcc
     4021 gcttacacag gtgaagacag gacgtactgt tttatgaatg cgtggattgc ctcgggtaga
     4081 atcactacat accacgagtg cgatagcacc ttataaccta tttttaagaa atgaaattgt
     4141 attgaggtgt tagatgcaat cgcgacgatg gatcccctgg gtgctgataa tggccgtgtg
     4201 cgtgacggcg ggctgtagca taatcccggc taccccggaa cctttgccac ccgacgaggt
     4261 tttaccgcca gaagaagcac ttcctcccgg ccctgaagtt gccgcgaagt tcgccgagac
     4321 gtacgactcg ctgcaggatt tctccgcgac ggtgacgacc gtatcggatc gcggaacgtt
     4381 ctgtgaagtg gtctggttta agaagccgga tcgattccgc gttgactacc ttgaaggaca
     4441 tcccaacaac cctgagcacc accccgcgaa ggaaggggat ctcgttgttt tcgatggcga
     4501 gacgcagtgg cactacgtca atgctaccgg tgaggttctc tacgtctctg cccggatgcg
     4561 caactatact ttcagtgaag agtacgcgac gggagctttc gacgctctcg gcctgatccg
     4621 gaacctgctt gctgaccatg agtgctcgtt atccggggtg cgaaccgagg atggaaagat
     4681 gatttacgtg cttgaggttg cgaacaccac ccggatagcg gacactccgg atccccgacg
     4741 gtacccggtc tacgccgtga atgccggggt tgagaaagat tcatggatcc tcaggagagc
     4801 cgacttcttc aacgccaggg gcgagactgt cctgatggta gattaccaca acgtcacctg
     4861 gaacaccggg atcccggata gcctgttccg gtacagcccg ctggaggagg cgatcgtcaa
     4921 gccgatgaga accgtggcga ttacccctcc ctacaccggc ccctgagaga agctgggtag
     4981 aagtacatca tccatgaagg cgacgtggtg cccgctctcc gagggacggg cgcgcccccg
     5041 gttccgggcc cgtagtgggt ttgttcgggt tcaaaatcta tcgggaaggc ccgacgtgta
     5101 gacgattgtc acagaaatcg tttatagaag aaggtcgacc tctcccgcat gggtaccgat
     5161 atcccggagc gtgggtccgg tcgtgcgacc cgtcaaaaat cgggccggta ttaccgggtc
     5221 gggacgactt accgggactg aacggtgagg gattgagaat gaacggaaag gctagaacat
     5281 tgaccctctt cctcgcggtg ctgctcgcgg tcgtcccggc ggcgggtgca ggtatcgatc
     5341 tcgatcacta cgtgatcccg gaactgaagg tgaacgagtc gatcgagacg atcgccatat
     5401 ccggggtgtt gagccttcag ccggaatggg acgggagtag aacctccggg ataccgttcg
     5461 gttcgatcat ggtccatacg gcggatggga ggacgctggt ctttgataaa gacgggaacc
     5521 agttgctctc catcagcgac gaactctctg caaaagttcc caccccgggc ggtgttgaga
     5581 aaccgtgcac ctggctgcac cagctcccga acgattcccg ggtctaccat cgtgacaacg
     5641 agacgttcgt ccttgatatt gccgggaacc tcatcctgac cgtcatcaat aagcatccgc
     5701 cgcccgtcga agacccgatt accatcaaca tcacctcccc gcgtgagggc gaggtggtct
     5761 ggatcgacgt cgtgccgccc cgcgtcgccg tcgtcgggga ggtgcatgcc cccgcaggtc
     5821 tgcacaacgt ggttgtgcgg agcggaaccg gagagatctc gtgcgggaac cggcccgaat
     5881 tcgcctgctc ggtgccggtc tccgccgggg agaacacgat caccgtcgtg gcggacaccc
     5941 atggacccct ggccgagaag accgtgaatg tgaccgtcca tatcggtctc ccgccgcccc
     6001 cggcgatcgc cgtctcgggc agggtgacgg acgccagcgg caacccggtc cccggtgcgc
     6061 tggtgcggtt cgagtcggtg tttacgctcg acgacgaacc ccttgctgcg gcggccgtga
     6121 ccggggagga cggcggctat ctggtaacca cccccggcta ccggcagact atcacggtcg
     6181 agaaggaggg ctactccact ctccgggagg agttcgtctt tgagaactca acaaacaccc
     6241 tcgacctgga actggaaccg tcgagccgga cggtcccggg gttcgactcc gtggtcgggg
     6301 ttctctcgct cgtgggaacg ttgctcctcg tcggtcggaa gaggcggtga gatgacacgg
     6361 aaaggctcgg taatcccggc gctcctcctt accgccgcag tcacggcgct ggcggcgggt
     6421 tttgcggtct tctggcccgg cacggaggtc gccggtaccc cgcacgagca gattgccccg
     6481 gaggatcagg ccctggtcca tatcgacttc ttcacggaga agagggatgc cccggtcgag
     6541 ggtgtggata tcctctcctc tatccggcgc tacgaactcg ccgtctacga tgcgcaggca
     6601 atccgggatc gcctgctttc cggcgaaccg ctggcgctgc atatcgccgg gcgaccctac
     6661 acggcggacc tgcaggagat gctgcacgac cccgaagccc gcagtgcggg gagacactcc
     6721 ttcaccggca ccctttccgg ggatggcagg gcgcatctga ccgtcagcgg caacgtcctt
     6781 cgcgcctatt tcgagatcgg cggcgtcgcg taccaggtgg agagcaccgg acggtatgac
     6841 accgtatccc cggagaaagt cctctactat gttttctcct ccgccgacgt gaaagggctg
     6901 agcggctgct tcctcacgct gtacaacatc agcaacgggg atctaaaaga ttccggatac
     6961 cctgcagact ggacaaaagt cgacttcctg aacgctggct acgagatcgt cccgctcacc
     7021 gatgccgatc ttgccgacct ccccagggtg aacgagaccc ttcgcaccgg ctttatggag
     7081 gtgccgctct ccgagaacga gacggtgcgc atcgcaaaga gttaccgggg gaagatcgcg
     7141 gagtactgcg ggaactacta cgtgatcgac ttcatcactg cctgatcccc ggccccctaa
     7201 accctctttg ctatcgtctc tatcgcctcg atcagcgtct gctgctgcgg cttgatgatc
     7261 gcgaccggac agtcaacgat cttctccacg atcggcgcga ggatgggggc gcagacgatc
     7321 ccgagcgccc cctccttgtc ggcccgcgcc gctgcggtga tgcactcgtc gagggagtcc
     7381 gccgggtagc cccggatccg gtacttccgc tcaccgatgg gaagatcccg gttgtccagg
     7441 tcgtcgagca ggaaccgcgc cgcgatcacc gcaatgaaac tcttctcccg gggctcgagg
     7501 acgttgacga tccgccggac ggttgagagg cgcggatccg ccctgcccga cgtgagtttg
     7561 tagagggttg cggacgggat ccccgcccgt tccgcgaggt cgcggatgct catatcctgc
     7621 cgtgcgagtt ccttctggag cgcatcggca aagtcggctt caaagatctt tcgcgagagc
     7681 attgttctac tatagtagta tctatgggat aatgtttttc ctgttctggt taagagttcc
     7741 gctatggaga acaacccttc tcttcctgca tgacaaccct cccgcatgga cccctcaccc
     7801 atccgcgaga tcacgatcct ccccggccgg aaccggaacg gcgaaccgga gcggttcgac
     7861 gcgatcgcca tccgcccggg cgacacgata tccatcgtcg gccccaccgg ttcggggaag
     7921 agcgccctca taataatcag gcaggagacc cc
//
LOCUS       DAJB01000131            3709 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_49429, whole genome shotgun sequence.
ACCESSION   DAJB01000131 DAJB01000000
VERSION     DAJB01000131.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_49429"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          643 a         1155 c         1183 g          728 t
ORIGIN      
        1 gggagcggga ggtcgaggta ggtgacctct ttctcgaggt cgccggggag gctgagccgg
       61 gggcagatca ggatgacggt cttcttcgta tccttgaggc tcgagaagag gtcccggacc
      121 ttccggacgg tgatcgggtc cgggttctcg gtgcggagat agtggtggta gtccgcgagg
      181 acgaagatcc cgttctccgg gtaccgctct atcgcttcga gaatcgagta ggggtcggct
      241 gggccggggt tgggctcggt cctgacgatc tcgtagaggc catcgtgagt cttccccacc
      301 tcgatctgcc gcccgtcgag gaccttcatc tccccggatg ctatctcttc gaggttctcc
      361 gtcaggtcga ggaggcccct ggtcacggac cagtacagga ggctccggtt caggccagcc
      421 gggcccgcgg tctccgcgag gatctgaacc acacgaggct cctcgtgcgt ttcgatgcag
      481 atcagggggt atttcgcccg gatgtgcatt tctagtgcat ttttgagagg cattttatcg
      541 gtatcggtca tgctgtttca cccatctcac ctgcatgtgc aggttggtaa cagagtgttg
      601 tcggttatta attatatagt tgccggtttg agtgcggagc cggtcggggg gatcgggggg
      661 cgggaaaaaa gggggttatg aggccttttt gcgagcatag gataaggttt ttttgcgaac
      721 atagaccata tagcctggcg cgaccttgac tgtgcgcaca taatacccgt cagcgcgata
      781 cgctttcgct gtccgttctg cttctttttt cgtgccgaac tgaccgtcgc caccgtccat
      841 cacgcctaac ttgtaccacg cgccgttgaa cgcccggcgg attgggtagc cgctgcaaag
      901 gccgggatca tactctctct gtcccatgta tatcacctcc ttctgggttg tgttctgcaa
      961 acggtgctcc tgcccccttc ggggccattc ctaacggatt ttctcaggag caagagggat
     1021 gcagaccggg cttcgccccg gatcaggggt cggatcggag atccgggaga aatgatccgg
     1081 gaagattctt gaacggggca agggagaggg agatccccgg acctatagca gctgccgctg
     1141 cttgaggaac ggcttgaggt cctctttgat gtagtatttc gcccccaggt cctgcagcag
     1201 atccacggcc tcgcgggcga acctgtgcca gtcgatggcc gcctcgatct tcgccatgtc
     1261 cccggatcgg gcgttcagtt tcccgacccg gaactgatcc acatactcat gcgtctcctt
     1321 gatgatcgcg agcgtctggg ccgggtcgat caccggctcg caggaaaccc acgtctcgat
     1381 gccgagccgg tgcgccgccc ggagggctgc gacccggccg gcgtaggtgt cggcgccggg
     1441 ctcgtagcgg gcgaggtcgg cagggtcggt gaacgtcagg gtggcggcgt aggtgctgtg
     1501 gtctttgtgc tccgcgagga ggtcgaagtc ccggatgctg cgccgcccgc ccttcgtcag
     1561 gatctggacc ggctgctcgt gccggaggag gatctccagc gcctgtcggg tgatcccgca
     1621 ccgctcctcg atctgcatgt agggatcgca ggtgaagcag agcaggatcg gcttctggtt
     1681 cccggccgcc tggagggcct tggcgtcctg gaccagcttc gcgatgatcc ccgcccgggg
     1741 cacgggctgg tggaagtctt cggtgtgcat gtgcaggaca tcaggcccgt agcagtattg
     1801 acatccggtg gagcatcccc gatagagatt gagggcaagg ggggaatact cccgcgcccg
     1861 gccccggggt tcgtagacga ccgagagcat catgccgccc cccggtacac ggttcccgcc
     1921 ttcggcaact cccgcacggc cccccggagg tcccgggccg ggatctcgag cgggggggct
     1981 tcgcccacat agacccggaa gaggtccccc tcccagaccc cgtagccgac ctccgtcccg
     2041 gcgccatcgt agaccggcgt ccggcgaccg acgaccccga agaagagcac cacatcccgc
     2101 cgggggatcg cgaggagccg ccctgcccgc ttcttcgtcg gcaggatcag gaccgcttcg
     2161 gcaagccccg ccccgatccg ggaccggccc ccgtccacgg tgccgtaggg atagcccatc
     2221 tcaggcctcc ggcacataca cgagaacccc ctgctggagt ttctctgcca aggtgtcgat
     2281 gaacggagcc acatcttccg tgaccgtata gcagtacccc gccccgccgt cgccagtcgg
     2341 cccgtaatgg ccttcgatga tcccgtagtc ctccagcgtg aacagggcga cccgggtaac
     2401 ggccttgctc atgagcgggg ccagccgctc gatcagaccg ggggtagtgg caggcgtccc
     2461 ttctttgttc atctgccgga tctcgatggc aagccggaag gaggcgctga gcgtgcccgg
     2521 gggccattgc cgggcctgat tattatctgt ggacgacatc ccgctcacac ctcctcggcg
     2581 gccacgatgt tccggtacca cgagaggggc tggaaccgcc agcctgtgca ggtgacatgg
     2641 taccgcccgc cctctttcag gctggcgtag cggtcggagg cgtcgaactg gagcctgaag
     2701 aggttgtcct caacggtgaa ggcttcgcct tcctcagtca ggatgaggaa gatcccgtcc
     2761 tcgccgtgcg ccccgatccg ctcctcgacg gtgatggtgg cctcggagac cgtgagcgcc
     2821 cccgcggtcg ccacccccgc gacgagcccc atgaggaaga agaagacgat cccgaggaac
     2881 accaagggtt cttcccccgg gtgccgcagg caccggaggc agaagagcac atcccggaca
     2941 aggtcactca cggcacccgc cccccgaaga ggacccccat tcccggcggc cggatcatac
     3001 tcacccagcc gaagacgaag gcgaggaaga gtttctcagt ctgggtcagg ttctcgatcc
     3061 cccggcccgc gttgatcatg tcgaggacat cagacctgag cgggcaggac gggatgacgg
     3121 tctcgaacaa ccagcggttc agttcctcgg cccggccagc cgggatcccg agggccgccg
     3181 ggacggacgc ggaatagttc ggcggcgggg ttaacggata gggttcagcc atttttatcc
     3241 ccatttttta ggatcaagtc agggctaatc cccgccattt caatggcggg atacagcccg
     3301 tccaccgata ccattatagg ccattatcac ctatattatg attatgagat tggggctgca
     3361 atctcaggga taacatccca agatgccccc gacttcagtc gggggagcag tcacatctgc
     3421 acatgcaggt taaccagagg tttgtctgtc atcatatatg tctgttccgc tttcgttccc
     3481 cggacccccg gcgggatcgt cgggaacctc ctccacgatc atcccggcaa cgaaaaccat
     3541 cttctttccg tcgccggata ccggccgccc gatgatggtg agccagtcca ggtagaggtc
     3601 gatctccgtc tcgggggtgg cgatctccgc cagtttgacg acgaacgcct gcaggttcgc
     3661 gagggtgatg gtggcgaacc ggagggcgtc ccccggctcg ccctcgacc
//
LOCUS       DAJB01000132            5082 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_131854, whole genome shotgun sequence.
ACCESSION   DAJB01000132 DAJB01000000
VERSION     DAJB01000132.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_131854"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          896 a         1551 c         1691 g          940 t
ORIGIN      
        1 ggtctcctgc ctgattatta tgaaagtaac aatcgtagcc tggggcagcg aactgctgca
       61 gttttccggt gcagcgcggg agatggggat cgaccttgcc gcgtggcagg tgcatgaact
      121 gacggacgat tcagagaagc gggagaggtg catcgagtcg tgcaacggag cggatgtcgt
      181 cctgatccac ccgtcgcacg acccggtctg ggacgagatc ctgggcggtc tcgcggaggg
      241 cgttccagtc ataccgttcg gctacaccga cgcctcctgg tcggcctcga ccgtcccgct
      301 tgccgtcgtc tccacggtga gcacctactt cctctacggc ggacctctga acatcgggaa
      361 cctgatccgg tacgtccagg ccgaagtggc cgggatggac gtcgcctacg accccccgca
      421 ggagacctgc tgggagggga tctaccaccc ggatgccggg tgcgtcttcg acgaccccga
      481 tgcttacctc gcctggtatc cgtcccggca ccgcatccgt gtcgggctcc tcttctcccg
      541 gacacactgg gtgaacgggg accttgccgt cgaggatgcc ctgatcaggg aacttgagag
      601 gtattacgac gttcttccgg cattctgttt cggggtcccg gataaggaga tcggggcgcg
      661 gtcggaccgg gagatgatag agaccttctt tgcgggccgg atctctgcgc tcatcgacgc
      721 aagaacgttc acccctcccc gtgacgccga tgcctgcacc gaggcgctcg cgaaactcgg
      781 cgtacctgtt tttcaccccc tgatcctcta tcaccggact gaagccgggt ggagggccga
      841 tacggagggg atgcgcggga gcgacgtctc ctggtacgtt gcgctgcccg agttcctggg
      901 cgcgatcgag atgctgccgg tcggtgccgc ggagagccgc gaccaggcgg gcaccgaggg
      961 cgagcgtcat gtccccatcg ccgagcgggt ggagaagttc gtaggcaggg tcaggaagtg
     1021 ggtcgatctt gcgtccagac ccgccgcaga acgacgggtg gccttcatcc tccacaacaa
     1081 gccctgtgcc tcggtcgagg cgacgatcgg agccggggcg cacctcgata cgcttgagag
     1141 tgtctcaaga atcctcgaag cgatgcgcga ccgggggtat gcggtcgaat gcccggggag
     1201 cggaaaagcc ctgatcgaga ctatcatgga cagaaaagca atcagcgact tccgctggac
     1261 gtcggttgcg gagatcgtcc ggaagggggg cgcccttgcc ctggtggagc cggaggagta
     1321 tgccgcctgg ttcgaaaccc tcccttccgg ggtccggacc cgtatctgtg aggcgtgggg
     1381 gcggccgccg ggtgaagagg tgaacggggt cccgccggcg atggttcacg aggggaagat
     1441 cgtcgtcacc ggggtgcgtt acgggaacgc gatcgtctgc gtccagccca agcgcgggtg
     1501 cgccggcccg cggtgcgann taccttgcga cctaccgcta catcgaggag acgttcggtg
     1561 cggacgtgat cgtccatgtc ggtacgcacg ggaacctcga gttcctgccc ggcaaatcgg
     1621 tcgccccctc ggatgcctgt ttccccgata ttgccattgg gaccatcccc cacctctaca
     1681 tctacaatgc cgacaacccg ccggaaggga cgcttgccaa gcggcggagt tacgcaaccc
     1741 tggtcgacca catgcaggcg gtgatggttg caagcgacct ctatgcgggg ttaaaagaac
     1801 tcgatgagca gatcgcggac tacaaccggg tgaaggacgt cgatcgggca agggcgcacg
     1861 cgcttgagca taccatcgcc gacctgctcg agcagaccgg cattggtggt gagattgggt
     1921 ttgcaaccct gcacgagcag ggagccccgt ttggagaggt ggtcgccgct gcccataccg
     1981 ccgtaacccg gattgccgac acccggattc ctgaggggat gcacatcttt ggggaggtgc
     2041 cggagggcga gcgccgggtg gagttcatcc atgccgtcat gcgttacgat ggggaagtga
     2101 agggtgcagt gctccggatg atggggtacg acgcttcgac tgcggacgac gccctgctgc
     2161 gggatgccga agctgcggca aaggctctca tcgccgcgtt tcttgaggga gatctccccg
     2221 accgggcggt ggaacgggcg ctctccggcc ttctcgtccg gcacgatccg gaagcactcg
     2281 aaaagatccg ggccggtgtc ctcgatatcg cagcgcggat agacgactcc gacgagatcg
     2341 ggagcctgct ctcggggttc gccggaggct acatcccgcc gggcccttca gggttgatca
     2401 cgcggggcaa gcctgaggtg ctccccaccg ggcggaactt ctactcgctc gaccccttcc
     2461 gggttcccac ccgtgccacg tggcggatcg ggacgcggct tgcggaaagc gtcgttgcga
     2521 agtacgtcga cgagcatggg cggatcccgg agaatatcgg catgtactgg atggcatccg
     2581 atgttatgtg ggccgacggc gagcagcttg cccagatctt ctccctcatc ggggtcgaac
     2641 cggtctggac ggacggcagg gtcaggtcct gccgtgtgat cccgcttgag gaactcggac
     2701 gcccgaggat cgacgtcacc gtcaggatga gcgggatcct ccgcgactgc ttctacacct
     2761 ctatcgaact cctggacgac gctatccggg aggtcgccgc gctggacgaa ccgcccgagc
     2821 tgaacttcat acggaagcac gccctccagg ggagcggcac ggcccggatc ttcgggagca
     2881 agccgggcac ctacggcaac ggcgtcagcc tggcggtcta cgcatcggcc tggaaggagg
     2941 aggcggacct tgcagaggtc tttgtcgggt ggaacagtta tgcctacggg cggggtagtt
     3001 tcggtgaaga gtcccctgag accttctccg cgcaacttgc cgccatcgac ctcaccttca
     3061 acaagacggt gaccgacgag tacgacctcc tcgggtgctg ctgctacttc ggggcgcacg
     3121 gcgggctgac cggagcggca agagcgctct cgaaccggga cgtgcctgcc tactacggcg
     3181 atacgcgtga ccgggaccgc gtggaggtca ggacgcttgc cgaggagnng gcacggctac
     3241 aagggtgccg gggacatctc gcggcaggtg ggcaccgtct acggctggga ggcgacgacg
     3301 caggaggtgg acgaccggat cttcgacgac atcacccgca cgttcgtcct ggatccggaa
     3361 atgcggcagt tcttcgcgga cgagaacccc tgggcgctcg aggagatcgg gaggcggctg
     3421 cttgaggcgc acggacgcgg cctctgggat gccgacccgg acgttctcga agggttgcgg
     3481 gcggcgtacc tggagatgga gggctggatc gaggaccgga tgggcgatgc cggcggcgac
     3541 gtccagggcg gcgcggtccg cgtagtgacc gtggaggagg caaaggcgct acggggagca
     3601 cgggagacgg agaaagaggc gtgaagacgg cgatggggat atccgttaac gcgtcgtgct
     3661 ccactggtaa tcgccttcgt gcggcacgtt cccgtttctc ctcattcctg gtttcgcagt
     3721 ctgagcaaac ataccgtgag gagcagtgca acagccgcaa aaattgcctc aaatcccggc
     3781 gacgcgggtg ccgccgggtc ttcgagtggg attgcagaca ggtcgtttcc atcgctcacg
     3841 acgatcgtcc tgccgtcggg agaccactgc tgcatcatgt atgccggatt atcaggatcg
     3901 aggggtgcga ggccggagcc gtcgctctcc atcacccaga gtttctggtt tcgcgactcc
     3961 gaccggaagg cgatcctggc accgtccggc gaccagaccg gtgatgtgtc ccagttcccg
     4021 gttgtcaatt gcttcgaacc caatccgtcg ctattgatta cccaaagaga gaactccggc
     4081 aatgtccccg gcggtgacgc gacgacgtag gcgatcctgg caccgtccgg ggaccaggaa
     4141 tacccggata cgctcccctc gctgaacgtc acctgtgtct tgcccgttcc gtcgctctgc
     4201 atggtccaga tctccctgtt tcccgacccg tccgagaggt atgcgatcct gtccccctgc
     4261 ggctgccact ggggcaggcg cgcatctcca tcggtcgtca gctgcttctt gttgccgccg
     4321 tccgcgtcca tgatccagat attcgcttcc gtgcggatgg ccgagccgtt caccgcaggc
     4381 gacccttcct ggatggccgc attctcaaag aggcaggagg tatagactat ccgctttcca
     4441 tcggcgctcc agtccgcccc gtagccggag agccccggac tgggggtgat gcggccgcct
     4501 tcggtcaggc gcaccctccc ggtcccgtca gggttcatcg tccagaggtc gagccctcct
     4561 ccgtcgtcgg acaggaagag gagcttcttc ccatccggcg accagggatc gaattcggtg
     4621 aagagattcc acgaacggtc gtccgtcacc ggtaccgggt tttggccgtc gcgctccatc
     4681 acccggatgc cggcatggac tccgtcggat gagacgaagg cgatcctggc gccgtccgga
     4741 gaccagaagg ggtatgcatc gaatgccgaa tcgtttgtca gctggactac gggtgcagcc
     4801 gctgccggga gagtgcacgc gatcagcagc aggacgatgc atatcgtttt tcggttcata
     4861 cagacctcat acacggtgtc accgatcaag gtgtcaacgt tctcccatat accttttctg
     4921 tgacaaacat cgaactgtcg cggctgtacc cgggccgtcg cattctcctg atacgcccta
     4981 gtgcatcttg tcagtttatt ttaggaattt tggcgtgtgg gggcgggcgc tatggtcgca
     5041 ctcccggggt actccggagc acggctttcc accgggtatc gc
//
LOCUS       DAJB01000133            3136 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_103970, whole genome shotgun sequence.
ACCESSION   DAJB01000133 DAJB01000000
VERSION     DAJB01000133.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_103970"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          515 a         1036 c          991 g          594 t
ORIGIN      
        1 tctcttcagt cccggtggtg gagcaccgtt gccgtctgtg ggaaaaatct tcatatctgg
       61 attatctgga atgctgcctt ccctccctcg aagcgttgag ttccagacag gcagtgtgac
      121 aatatatatt ttaccctgct tacggccgtc ggcttgccga ccctggcgca tacggtggcc
      181 ccggagactc cgaagttccg ggtggggcac gaaatggcac cggaacccgc gccgccccta
      241 actattaact gcttttacat cgagtatgta tctggactgt caggcggcag atgatgagag
      301 ttacccccac tgaccggtga tgaagcaggg gactgatgcc gctgccctat gtataattgc
      361 tgtttatttt tcccgcacgc tcgccccgca gttcctcgcc cgcgtcagaa gcaccgagcc
      421 cccgtggtcg ccgaggacct ccgtcgccgc ccgggccaca tcctgcatat ccgtatcccc
      481 gacggcgtaa accgtcgggc caaatgaact taagcccgca ccggcggcgc cggcctgcac
      541 cgtagcctcg aggagcgttg agacgaccgg gtgctgcatg gccacctcga ccttcttgaa
      601 ccccagatgc tgtgtccggt tgatcgccga gccgaagagg tcgaggtcgt gctcgatcag
      661 gcccgggatc atccgcatca gcacctcatg gcagacctcc cgcacctcat cgagcggcac
      721 cgggcagtgc gtgcggaaga tatcgacctc ccttcctccg tgcgcccccg caccggtatc
      781 gggggtaacg agcaggatgc gccagtcctc cgggaaccgg tgccgggcga gaaccggcgg
      841 gggcgcgatg ccccgtgatg ctgccgaggg gcggaaatcc tgcttgccct cccgggagcc
      901 gaaccggtgt ccgccgtcca ggataaaccc gccctgctcg aacgccgccg tgccgatgcc
      961 ggaggtcccg ccacggccga cgatcttcgc aaggtcggat accgggagat cgcggtcgta
     1021 gagccggcag agggcggcgg ccgtggcgag cgcaacctgt gtcccgctcc cgagcccggc
     1081 atgctgccgg gcggtggtat gcagggtgat ctccgcacct ccggggagtc cgagcccctc
     1141 cgtcaccgcc cgggccgcat cggcaaccct gcggcgggct tcctcatcgc cgccgtgcac
     1201 gtcggtctcc gtgctcttcc gtgcatccag gatgcacccg ggctcgtcga gagtgacccc
     1261 taccccgccg tccaccctcc ccgaggcacc gttcatatcc agcagggtga tgtggatccg
     1321 ggacggggcg tcaacgagca ccctgatctc gtctgtaaac gccgtgcagg ggaagatctc
     1381 ctcgatggcg atcaacggct cttcgcggtg aatgatccgg tatttccggg agagcatcgg
     1441 ttcgcaccga tggacgttga atgtccgggc cagatccgca ccggcatgga tgacgcgggc
     1501 atcggtgatc tcccggcgcg actctatctt gtggcggccg agaattctcc cgatgggaat
     1561 atccgcgcgc ataagatcct gcctgaactc cggcgcaagc cgccggagcg gggtgcagga
     1621 gaccgcatag atgaggacct ctcccgttac gctgtcctgt aactccacaa cacggtagtt
     1681 gacctcgtcg cctggctcga tatcaagtgc cgctgcggtc tttatgtcgg cggcgacgac
     1741 atcctgaacg cgggtggtga tggtgaccgg atgacccgtc gccatctcaa gcaggttggt
     1801 caccgatccg tcggtcccga tgaggacctt ctgcatcggg gagagccttc ctacgccctg
     1861 ctcgatctct tgtatcttct cgacgatcct gtctgccgtc aggggtttca tatccgaata
     1921 gatgtgatgt atggcgacta caaaaaggtg gtgtcgggtg atcccggccc tgccggccgg
     1981 ttcacctcga gaagagcagg gagaggaggt cgggcgcctg catgagtccg aatgacccgc
     2041 ctgccgcctc gcgctcggag aacgcggcag cgtcgtcctt ccccttcccg agtattgcaa
     2101 acggtatcgg gtccgcggtg tgggttttga gccgtatcgg cgtcggatgg tcggggagga
     2161 ccgcgacggt cgtatccggt cggtcgagga caatcgcgat cgcctcgtcg agccgctcga
     2221 tcgcccgcac cttctcctcc acgcttccca tatgtccggc ctcgtcgggg gcctcgacgt
     2281 gcatgtagac gaagtcgagg cggtcgaggg cgtcgactgc ataccgggcc ttcgcctcgt
     2341 aatcggtgtc gaggaacccc gttgcacccg ggacgcggat cacctccatc ccggcaaggc
     2401 gggcgatgcc gcttaagaga tcgaccgcgg agatcacggc gccggagagg ccgtacttct
     2461 ccgtgaagga cacgagcgac ggcttcctcc cgccgctcca cggccagact tcggttgccg
     2521 gggcctttcc ctcccggatg cggcgccggt tgaccgggtg gtcggcaaag acctcccggg
     2581 accgcttcat gcattcgagg aggataccgg catcgtctcc gcgcgggagg tagtccccga
     2641 cggactggcc gacgatgtcg tggggcggtg tagtgacggc accgtgcgcc ctggagacga
     2701 ccatcaggtt ccggtaactg acccccgggt agacccggac cctgccgagt gcagcgtcca
     2761 ggtcgcggag gagttcggcg ccctcggcgc tggagatgtg tccggcgttg aagtcctcca
     2821 tcaccccgtc acggaccgtg accaggttgc accgataggc catctccccg tcctggaggg
     2881 tgactcccat gctggcggct tcgagcggcc ccctcccggt gtaggaggtg cgggggtcgt
     2941 agcccaggat ggagaggttc gcgacatcgc tcccaggctc aaacccctcc ggtacggtcc
     3001 ggagcatccc gcaccgtcct tcccgtgcga tcctgtccat attcggtgtc tctgcgtatt
     3061 caaggggtgt cctgcccccc agttcggcaa gcggctcgtc cgccatgccg tccccgagaa
     3121 tgatgatgta tttcat
//
LOCUS       DAJB01000134            3438 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_91627, whole genome shotgun sequence.
ACCESSION   DAJB01000134 DAJB01000000
VERSION     DAJB01000134.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_91627"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          565 a         1156 c         1116 g          601 t
ORIGIN      
        1 cccgggcgcg acggcttccc cccgtctccg ggaggaaaga agcgatggcg tgcccggtcc
       61 ggagggctta tgccagggct tcgtgacgct gctggcagcg tgcctgcagt tcatcccact
      121 cgtccgggtg ctcctccgcc cactcctgga tcgcatggac tatctgaccc cgccactcgg
      181 ggtccccccg gtagtttatc aggatgcaat caaccactac ttcgatctgt tcttccggat
      241 tttcattgtt cgttatcatg ctgcatacct tctgtcgtgt gcccccggtg ctcatatcgg
      301 gtgaagatcc gacaccatat aatcctgatc ctcctctgac agcggcgccc ggcggtctct
      361 cggttgcctg aaaagagacg ttaagccccg ttttttgccg ccagtccgct ccctggccgc
      421 gtgctggcaa cgccgttgca gggttttatc tggctcccgg ccgaagagct acgggaacaa
      481 tcatgaaact cggcgtactc ttctccggcg ggaaagactc caccttcgcc tgctggaagg
      541 cgatgcagca ggaagaggtg gtctgcctga tcacggtcat ctccgggaac ccggaaagtt
      601 acatgttcca caccccgaac atccgcctcg ctgcactgca ggccgaggcg gcgggtctcc
      661 ccctcgtgga ggtggagacg gcgggagaga aggaggaaga actcctggat ctcgcgcggg
      721 ctctcctcgc cgccagggag cagttcggga tcgaaggagt cgtcaccggg gcgatcctct
      781 cggtctacca ggcggcacgg gtccagcggg tctgccgcga actggacctc tggtgcttca
      841 acccgctctg gaacgtggac caggaggcct acatggagga actgatcgac gcggggttcc
      901 ggatcatcat cgcaggcgtc ttctcctccc ccttcgacaa atcctggctc gggagggaga
      961 tcgaccgacg caccctcgac gaactccggg agatcgcccg gaagcaccgg atcaccctca
     1021 tcggggaagg aggggagttt gagacgtttg tcatcgacgc ccccttcttc tcctggcgga
     1081 tcgcgatcga ggaggcatca aaggtctacc ggaactacaa cggcgtcctt cgtatcgagc
     1141 gggcggggct ggtggcaaaa tgatactgtt aatcgacctc tgctaccggg ccggctcgct
     1201 ctcccgggac gagttcgtcg ggccgatcga acggcttctc cgggaggcca aaactccgtt
     1261 tttcacccgc cactacaccg ccgtcggcgc accggagatc gaggccgcgg atggggtgat
     1321 cctctgcggg acggcgctcc gggacaccgg gtttgcagag catcctgaac ggttcaggtg
     1381 gcttcctgca ttcgggcgcc cggtgctcgg catatcctcc ggcatgcggg ccctcgcggc
     1441 ggccttcggc ggcggcatcg agccgtgctg cgagatcggc atgaccgacg tccgggtgct
     1501 cgcgcccgac cccctctttg ccgggaggga ggatttcccc gcatacgggg tgcatgaatt
     1561 cgccgtgcag gtgcccgacg agttcctccc cctcgccgcc tcggaccggt gcacccaggc
     1621 gatccggcac cgctcgctcc ctctctacgg cctcctcttc cacccggagg tcaggaacga
     1681 gtggatcgtc gagcggtttc tctggctggt aacgaccggt cagtaacacg tatccccgga
     1741 ccccgggtaa aagggtagcg atgctccggg atacgccccg gcgcacctcg cacggggatg
     1801 aagcctgggt tacccagcac ccggaccctg ccggcggggg ggtgaaggag tgcccgaccc
     1861 gctcaggctg ctcgtcatca ccgggagccc gggcgacgcc cccctggtcg gagaggccct
     1921 tcgcaaggcc gggctcccgg ccgagacggt cccgcaccgg ctggttgccg aaggcatcga
     1981 cgccctccgg cgagagtcgt tcgacctgat cctgatcgac cccggccggg agcccggggc
     2041 ggtgacccgg gtgagtgaag aagcctccga taccccggtt gtcatcctgg cctcgcgcga
     2101 agacctcgat gcggcggtcg gggctctctg gcacggtgcg gacgactacc tggtccggga
     2161 gaccatctcc cccgaactcc tggtctgcac cgtccggcac gtcctcgcgc tgaaacgtgc
     2221 cgaggtggcg ctcggcagag tggagcacct gtgacgcgag atcggcagga gcctgcacga
     2281 aggggttctg gtgctcgacc cgggaggcac cacgatcttt gccagcgccc ggatggcgga
     2341 gatcctcggt tacgccgtca gccggataca gggcgcctct ttcctctcct tcgtcgaccc
     2401 gggcgacgtc ccgcgggcga agacgctgat cgagcagggc gagtggcggc gggcggaggc
     2461 cgaactccgg ctccggcgtt ccgacggagc ggcgatcgac gccctcctcg ccgcctcccc
     2521 ggtcaccggc gagagtcccg ggtacctggg cgtggccgtc ggtgtcatgg atgtcaccgg
     2581 acagaagatc gccgaggaga aggcccggcg caggaacgcc cagctctacg tcatcaacca
     2641 ggtggtcagg accgccacgt cgtcggcggg catggacgaa ctgctggcag gcgtgctcga
     2701 gaagaccctg gaactcctcg atttcgaagg gggcagcgta tacctgatcg accccggccg
     2761 gtccagggcc gaactggtcg ctgagatcgg gctcccgaaa gggttctcgt ttcggcggcg
     2821 gatcgtcgac ctgaacgccc ccccgtacga tgcggtcctc gggcagggta tggcgcactt
     2881 cgtggaggac tacctgcacg cctacccgca ggacgccggc gcaggaatcc aggcgtttgc
     2941 aagcatcccc atcatcgccg gggagcgggt cgtcggcgcc gtcaatctgg tcagccggaa
     3001 cgtgcacgtc ttctcccccg acgagcggga cctgctcgcc tccatcggcc gtgaggtcgg
     3061 gagtgccgtc gagcggatgc ggctccacga gcaggcgaac ttctacttgg atctcatgac
     3121 gcacgacatc aacaacgcaa acaccgtcgc catcggctac gcaacgctcc ttgccgagac
     3181 gctctccggg ccggagaagg accttgccgg gaagctcgcg gccgccgtca ggcagagcgc
     3241 cgagatcatc gggaacgtct cgacgatccg gcggatcgcg gaagaggccc tcgtgcccgg
     3301 gccggtgaac ctcgaccaca tcgcgaggag cgtcatctgc ttctttcctg acgtcgatat
     3361 ccgctacctg ccggaggacc ggtgggtctc tgccgacgac ctcctgtcgg cggtcttcgt
     3421 aaacctcatc ggcaacgc
//
LOCUS       DAJB01000135           13125 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_39191, whole genome shotgun sequence.
ACCESSION   DAJB01000135 DAJB01000000
VERSION     DAJB01000135.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13125)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_39191"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    12822..12926
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2151 a         4156 c         4210 g         2503 t
ORIGIN      
        1 tttcaaccgg gggtagttga ccctcttttc ttacggtaca cgaatatata ataccctgct
       61 ccgatgataa tggctgcaag gacgacgagg aagaccggat tggtgaagaa ggcgccctgt
      121 ctcctctcga gcgcgacctt tactttcatg gtatctgaga tcttgctgtt gtcgagttcg
      181 tcacggtagc ggatctccga gtcgatcccg tactccttct cggtgccgtc gttatcgaca
      241 ttcacctcga accgtgccgt cgtggtctcg ccgggggcaa ggtcgccgag gaaggcggta
      301 tcgtcgctgc tggtgaaggg gtcgacagcg ctgatccggg cctgggcgtt gtagaccgtt
      361 gccgcgccgg cattccgata cacgacttca aggatgctgt tactgcccag gtagagcgtt
      421 gataccggcg agacgacctc gaaggcgatc ttgccgccga ccgggagtcc gatcgtcgcc
      481 ggctgggagg tcaccgtctt cccttcgtag tcctcataag tgactgcgat gtccagcgga
      541 tacgactgag gttcggcgct gtcggcaacc gagaccttga acctcacgtc cactgtctct
      601 cccggctcaa acgtcccgat gtaggcgctg ccgtcggtgg ggatgagcgg gctcgcgtcg
      661 tttcttacga cctttgcgat ggccttgttt gccgtgtcgt gcccgatgtt cttcaaggtc
      721 atgtagacat agccttccgt gccgacgttc agcgattcgg accgtacgtc caggacctcg
      781 acgcggaggt cgggcgtgac ccgcacggtc agcggcaggg tcagggtctt cttctggtag
      841 ttgtagcgga gggcatcccc gtaatcttcg gcatcctgca ggtaggtgta ggtcacctca
      901 agcggcagtg tgtaagtgcc cggctccgcg gaatctgcca ctttcacgtt gaacgtagcg
      961 gtcgcggatg ccccgcccat gatatcgctc aagaactggg ggtccgtctt gatggtaaac
     1021 ggcgtatcgg tgcccttgag cgccacggta accagtttcg cggtgttcgg ctggtcgtcg
     1081 ggggtgatga gcgtcgagcc gaccatcttg aaggtattca ggccgctatt ggatatgatg
     1141 accgcaagct gggtctcggc accgggtgcg aactcattgg tgccggcgat tgttgccgac
     1201 agatcgggac ttccatagag gtacctggtg ccctgcgccg cccccggcac aaccaggagg
     1261 gccagcagga gcatcagtac cgttacgtat tgccaacgca tctcttcacc ttgttgttat
     1321 agtagcaaca acatttatcg ctgaacggta tatatacgtg atggcatgaa tgctccccgg
     1381 gatattccaa ataacctcct tgaatctctt aaatcactgg gactcacgaa atatgaagcc
     1441 cttgtatata tcgggctcct cagggtggcc ggtgcgaccg cgacggagat ccacgagatc
     1501 tccggcgtac cgcgcgcgtc cgtctacccg gttctcgacc ggcttgtcca gaaggaactg
     1561 gtcagcgtct cgtacatcac gcccaaacgg tttgacgcgg tccctcccga ccggggcgtc
     1621 gagaacctga tgcgccggat cgagaccgac gcgaaaagcg cccgggcggc cctcgacgca
     1681 ctctaccggg agaagaggcc ggaaggccgg ggcaaccagg aacagatctg gacgatctac
     1741 ggggaggaga acatcaggac ccgccttgcc ggtatgttcc ggagcgcgga acggagcgtg
     1801 gaggtgctgg cgacatggga tctcctcccg gagagcctgc tcgcactgct cgaagccgtt
     1861 cccgactccg tgtcggtcga tatcgtcacc gactgctggg agggcgagcg gccctcccgg
     1921 ttcgggattc atgtgcttcc cctcgcgacg ctgcgcgaga ctcgttcccc ccccggcaaa
     1981 ctgcttccgt atgagagctc cggggtcttt ctggtggacg acgtccgtgc gctgctcagg
     2041 gtagggtcgg gtagcgagca accctcggcc ctttactcgg agtctgcggg gctcgtcctg
     2101 tttgtcaggc ggcatatcac cagcgtcacg gaatgggcga ggaccgccgc tcagtagtcc
     2161 tccagataac ccatctcccg gatatcgatc agccctttaa gtgcccgggt caccaccgcc
     2221 cgtcctgtct ccctgacggc ggcgagcgcg ttcgtgcccc cgaccatcgc cacacccaca
     2281 tactgcggac tcaccgggac cccgagcagg gggacgttcg gggcgccgac atcaaggatg
     2341 ccggagaacc cgatggccga aagttcgtcg agcaccgctc ccataagcgg ttcggcctcc
     2401 atgtggcatt cccggatgtt cgcgaggatg ttgccgtttc cgtggtgcat gacgtcgagg
     2461 atcgacgttg cctcctggga tgtcaggaca tcgagggggt cgagcgtggt gtcgcggtag
     2521 aggatcatgc tcgtgaaccg ctgcgcgacc ctcccgtcga cctcgacgat ccctcccccg
     2581 atcgggttga gcgggatgcc gcgccggagg aggagagcgt cgagcgtgat gctgcacatc
     2641 gtgcagactg cgtgccggcc gtccggcaca atgtagccat cgacctgctc ccccggcccg
     2701 gcgagcagga tcctgtcgct gatggtgata cccgttttat gggcctcttt catgatggag
     2761 agcgcgaatt cgacgtcgtg gctctcgatg agcgagaggt tgtagatgat tgtcccgacg
     2821 tcctcctccg gccggtaggt caccctgagc gcgtactcct cgatacggtg attggtgaac
     2881 ttcaggggga catgcattat cagatctctc gcacggcagg ggaaaaaagt gttgccctcg
     2941 gagtcgggca gagtcgcccg gcaggaggtt cccgggggat ccagatccaa cccgggtttc
     3001 gccggttgcg gctcgcaccc tgccgtcatc cgtttttccc tctgtcccgg attgtgcgca
     3061 gtcccacctc ccacccggcg gactcccggg acctgtcccc cggcccgacc gaccacggat
     3121 gcaaaacttg ttctacctcc ctgtggtatt gtacggtgaa tggagagtgg gactcgggaa
     3181 aaagcccgga acgtgctgaa gcggatcctg atagcggctt cctacgacgt cgaggaggtc
     3241 ggcaacccgc tggatctctc cgcaatcggg agtgaagacg ccctggtggt gctctgctcg
     3301 gacgacccgg agacgattga gatcttcgat tcgatgactt accgcctgcg caccggcgac
     3361 gacaaccggg tctgcaggaa attactcttc gccctcgatc cggcggtgca gacggaggac
     3421 tgcatccggt ggggcagcga cgagttcgcc cgttacgccg gcagggccgc gcttgccgag
     3481 gtgctcgggc agagcctggt ccttgacttt ggctgcccgc ccccggcgat ccggcgcgag
     3541 ccggaaaagg ccggccccgc ggcagtggag gaagaaacgg aagcggggcc ggagttgccc
     3601 caccttccgg tacaggtcgc tgaaaagcgc gcctgcggca tcgccgggct gcatgggtcg
     3661 gcgaagtgcc ggttcatccc gtactggagt taccactacg tgagcaccgg cgagcgcgag
     3721 gtgaaggacc ggctggtctc attcgacgcc gaaggagccg gagccgtcaa tgccatcaac
     3781 ggcatgagga tggagatcga ccccggcacc gtcgagcgcg gcccggtccc gttcggctcc
     3841 gagatcgtgc cctcacggct cgtgcgggag gacgtggagg agcagctcgt gcgtgatatc
     3901 gtcgaacggc tcacccagcg ggtgcggttc cgctacgaga agggcgacgc catcttctac
     3961 gaggaaaaga ccctcaaacc cggccggagc aacatcaagg tcgacctcga gaccgtctac
     4021 gtgccggtct ggcaggtgcg gggcgggagc aagatcgtcg aggtcaacgg cttcaccggt
     4081 gaggttctct cgatgccgat ggacgagggc gtcgagctgc tgtaggtaac catgatcgtc
     4141 gtcatcggcg gcgggcccgc aggaaggctt ggggcgatgc acctcgcaca ggccggggcg
     4201 gaggtccggc tcattgagca gcgcagcatc ggtggacagt gcctccacga gcggtgcatg
     4261 acaatctgtg ccttaaacga cgtcgcccgg gtcatcgagt acgcccggac ccagaaggat
     4321 ctcgggatcc tcgactcggt tccggcagtc tcctatccgg cgatccggcg acgggtctgc
     4381 gaggtccagg agaaactcgc ctcggtcctc gacgccgaga cccgccgggc gggggtcgaa
     4441 gtcatttacg gagccgttgg ccgcctggaa ggcaaccggg tctatatcga tgacgaggag
     4501 gtccgggccg acgcggttct cgcagccacc ggttcgcggg cggccgtccc cactatcccc
     4561 ggcaccgatc tccccggcgt ctacacctac cagacgctcc ccgcgatgtc cgacctcccg
     4621 cgccgcatgg tcatcgtcgg cggcggggtg gtggcggcag agttcgccca catattccgg
     4681 gcgttcggct ccgaggtcga gatcgttgcc cggcacgagc tgctcgggga cctcgatgag
     4741 aagatgcgct ccgccgcgct gcgcgacctt gcgggggttg gcgtccgcga gcagaccgat
     4801 gtcgcaggca tcgagggcga cgggagggtc cggtcggtcc ttcttgccgg tggcgaggag
     4861 ctgtcggtcg atacggttct cctcgccacc gggctcgttc cccggtcgga gtcgctgcag
     4921 ggtctccgca agggcccgga cggcgcagtc gtcgtggacc ggcggatgcg cacgagtgta
     4981 gagggcgtct acgccgccgg cgacgtcatc ggcccgcctt acctcacccc tgcagcccgc
     5041 cgtgagggcg tggtggcggc cgagaacatc ctcggccacc ccaccgagat ggattacacg
     5101 ggcatccccc aggcgatgag cctccgctac gactatgcct ttgccacggt cggcaccgac
     5161 gggggcgtca ctctctcggc ccccgccccg gcgggccccg gctcgttctg ggacgtggcc
     5221 gggggctggg cgggactttc ccagatccgg gtcgatcccg ggagcggccg gctccttgga
     5281 gccgccgccg ctgtccccgg agcgtcgatc ctcctctcct accttggcta cctgatgaag
     5341 cggggcatca ccgtcgacga cttcgacgat atcgtcgagg ttcacccgtc gaccgacggc
     5401 gtctatgggc tcgcccgcta cgctgccggg atgctcaaaa agaaagaatg atccgtcccg
     5461 gcggctctaa atctgccgga acatctgccc gaatttcggg ataaaatctt ttttccggga
     5521 catgacaccc tcaagccgga ctggctggtc ccgcaggtcc agtttggtga tgcaggcctc
     5581 gtccccggcg gcgaagaggt cgctcgcgtt actaaagacg tttgtaaaga gggtgaagaa
     5641 gcagtcgacc cccatcgtcg tccgcaggcg gtcgagttcg gcccggatct cttcggcatg
     5701 ggtctctgcg aacgcaaacg acgacgtcat cacctgcgag accatgatgc tccggccgaa
     5761 gaggttgtag cgcttcatgt cccgtgtgag aagttcttcc ttcgggaggc tatcgaggtc
     5821 catgcccttc tggatcagtt ccgggccgaa cttggccggg tcgaccccgg cgatcgccgc
     5881 gaggtactcc gctgcccgga tatccgcggg cgtggtcgtc gagagtttca tcaccattgt
     5941 gtccgagagg atccccgcga ggaggagacc ggcggtcgac ggcgtgggct caatgcaggc
     6001 ctcgatgaac ttgtttgcca cgatcgtcga ggtcgacccc acggggtcgt tgaggaactt
     6061 caccggcttt aaggtggaga tcgcgccgag gcggtggtga tcgatgatct cgaggatatc
     6121 tgcctgttcg atcccgtcca cggcctgcga gtactcgttg tggtcgagca ggatcaccgc
     6181 tttctggacg tcctgcatca gtgtcgtccg ggatatgagc ccgatgtgct gccgcccttc
     6241 cccgacgacg caggctgtcc tgaacttgga gttcgagacg acccctttcg catactcaag
     6301 cgagtcctcc atccgcaccg tcgggacgtc ggtctccatg atcatgctcg ccggcaggga
     6361 gaggctgatc atcttgccgg cgctgaaggc gtcgagcgtc gtcgcgagca ccgaggcccc
     6421 ccgcgtccgg gcggcggcga tcacccgctc gccgaccggc gccccttcgg cgatgatgag
     6481 cgcggcgacc cccgccgata ccagggcgag ctgggccggt tcattgtccc cgacgatcgc
     6541 gatgtcttcc ggcgtcatcc gggagagggt gacgtgcagg gcgtcgatca ccgtgtagac
     6601 ccggctttcc ccgagcgtct cgcacgccgg tacgaccgtc cgggcgtcga ggatgcgggc
     6661 gagggtctca agcggcatcg ggatgaggga gagctgctcc cgggggttct tccggacgta
     6721 ggcccgggcg agaccgtact cgctgacgag gccgaccaga gtcccgtccc cgtccgtgat
     6781 cggcatgttc cgcatgtcgt aggtgtccat cagggcggcg acgtcgatcg tcgggacgtc
     6841 ctgccggacg ctccgggtat cgagcggtat atccgatacg ctgggctcga cgcttgctac
     6901 ataaaccggt gcctccgcgt tgaacgtctc gagcgcaaac ctcgtctccg ggctcacctc
     6961 cccgcaccgg gcgggaatat acttgccggg ctccgcgtgg ttacggagct cggcgtagcc
     7021 gatgacgctg cagatgctgt cggtgtcggg ctgcctgtga ccgatgataa cgatattgtt
     7081 ctgtcccatg ctctcctcgg atccccggcg gggcgacggt tgtctcaaga gttatcgctc
     7141 tctgccgtca aaggttttat gccggcctcc gggagcaacg ttgaagtagg ggttctctcc
     7201 attcttccgt gtgggaaccg atcgccccgg gtccgtcgcg gccctgctca tcctggtctg
     7261 cctcctcgtc tcccccgcgg ccgcggcccc gggagacgtg acggtgacat ccaccacggt
     7321 cgacccggtg gtcctgatgc cgggtgatga ggggaccatc acggtcgtcg tgcagaatac
     7381 cggctcttcg accgttccgc tcggcggggc ccgcctctac gaggacggtg tgctcccggc
     7441 aagcgacccc tacccttcgg tcggggatat cggggcgggc ctcaaccgta cattcacctt
     7501 cgccgtccgg gcggatgctc ccgatggtac ctactacccc cgcttctccc ttgacctccg
     7561 ggagaacggg accctcagcc acccggtccc cgtccgggtc gacggcacgg tgctccaggc
     7621 atctgtggcc gggaggccgg acgccttctc cgaaggacgg acggccgccg tcaccgtccg
     7681 ggtcgggaac ccccgcccga acgccgcctc cggcgtccag gtgacgccgc tcggcgaggg
     7741 ctttaccgtc accccgaccg gggcgtttgt cggggctctt gcccctgacg gtactgcgac
     7801 cgtcctcttc aacctgacac ccgatctgga gacgaacgtc accttccggg tggtctggcg
     7861 caacggcatc aacacccaca ccagcgatct cgttctcccg atcgccttcg gcgaggataa
     7921 acagcgggcc gatccggtca tcacgaacgt cgaggtcgcg cccaccgccg gggctaccgg
     7981 gcgacgggcg acgtcatgaa cgcgggcctc gagtccacgc ggtcggtgct cgtcaccgcc
     8041 ggtgcgcccg cgacgcctat tgacccgttc cgggtctacg ttgtcgggac cctcgacccc
     8101 gacgacatct cgacgttcga ggtgacgttt cagactgaca caggcgtgac cgagatcccg
     8161 ctcgtcgtcg agtaccggga cgacgatggc aacctctaca ctgccacacg gccgatctcg
     8221 cttgagaacc gcaccggaga ggaggacggg gatggcggga tactggtccc ggccgccgtc
     8281 gccgggtatg cccgccgcac gatccggatc gtggacggga gattggcgtg atgttcttct
     8341 cgttcgcggt caggaatctc cggaggcact ggatacgctc ggcgctctcg atcatcggga
     8401 tcgtcatcgg cgtgacagcc atcgcctctc tcggcatcat ggggagcagc ctctcggtgc
     8461 tcgtcggggg gatggtctcg gatgtgagcg acaccgtgct cgtcacaccg catctggcgg
     8521 catcgagcgg cgacccgttc gaccccagaa acacccttgc cgcgcgcatc tccgagagag
     8581 acctggggct gatagagaag gccgccgggc agcaccgcgt catccccatg attctcgcgt
     8641 cggatcggct gcaggtgcgg gacacagggg gctacgtgac gatgtatgcc ctgcctgcag
     8701 aagatatccc cttcctgctg caggaggaag ccgggatcta cccgcaggcg aggtcttcgg
     8761 gggtgatggt gggcgcgctc cttgccgacg aacttgacct gcatccggga gccgcattga
     8821 cgtcggcggc gaaagcgtcc gggtcgtggg gatcgcggcg gagcggggga tgggcatcga
     8881 catcaacccc gactatgccc tcatcgtcac ggaagactgg tatacggagc ggcgcgggga
     8941 acaggactac agccgggtcg tcgtgaaggt cagcgatctc tcggagatcg agggggtgaa
     9001 ggccgctatc gaccagcaga tcaaccgcag aaaagaggtc gtggacgttc tggactctcg
     9061 cgagatcctg gatctctact acgagacctc cgacgccatc aacatcttcc tcctcggcat
     9121 aggcgcggta tcgctcctcg tcgccagcgt gagcatctta aacgtcatga tcatatcggt
     9181 cacggagcgg acgggggaga tcgggctgat gcggagtatc ggggcgcgaa ggagggaggt
     9241 gctcttcatg ttcctcgacg agagcctgtt cctcgggatt gcgggaagcc tcgtcggcgg
     9301 catcgtcagc atagcggtcg gctactatgt cagcgcctcg gtagcggagt tctttacgga
     9361 cttcgccgta tcgggagggg gcggtgtccc cgtttcggtg gtgatcggct acgttgcctt
     9421 tgcgatggcg ttcgggacgg taacgagcat cctggccggt ttctacccgg catggaaagc
     9481 ggcgcgcctg aatcccatcg aggcactccg ctacgagtga cggtcttctt ccggacacgg
     9541 tcccggaggg taaggaggtc gcccccacgg gatcccctgg tgctggattt ttatacctga
     9601 ggcggcatgg gggacccggt gaagacgatg ctttttgtcc tgtggtatgt gttcgaacct
     9661 gagcacaccc accatgtgct ggaactctgg aagcacttcc agttcccctc cgatgtaaag
     9721 gtccaccacc gctatctcct tatcgggagg cacatatcgg ttgctgtctt cgaagcgccg
     9781 gacgagcgct ccatcttaaa gattaccgcg ccgttcagct cttacggggt tgcacacgtc
     9841 gcgccggcga tgtcgctcga agaggcgatc aagacggtct gatcggggca ctgcacaact
     9901 gaaacagcac cccactattt tccaccctgc gggatcccct cgcgtctcct gtcggggcac
     9961 tcccggaacg ctgccgtttg ccacgagcag gacggaccgc cgcgcccggt ccgggatgtg
    10021 aacccccggg tgcctctcct cctcctgaga gcaggtcgta agagcgatca tggtgatgcc
    10081 tctccccgtg atgagatcat gcgtaatttc atagtcccgg agaccggaaa ctctatgagc
    10141 acttgtagga tgatagactt ccctgtcagg gagtgctctg acccccatcc ggtcctcaat
    10201 acgatagcaa taccccagtt gtgtgaaacg aatgttacag atgattacgt ggctcgacaa
    10261 aaacttcagc tcgcttcagc cgacgcgggc gatcatcatg agagcgctgc gccacctgcg
    10321 ccccgcggat cgaaaaaagc tcttctccga ggatatcccc gagatgcgga ccgccgaagg
    10381 gcggtggttt gaggcgatcg tctacgagat ggtcctcgac ctctcactcc ggaccgacct
    10441 catccgctct gtcgtggccc ggggggcaga cgggcctgga aaggtccggc gggcgcaact
    10501 tggccagaac gggctcttct actcgaacat cggggatatc aaggtccgtg gcaacggtca
    10561 ggatcttgcc gaagtcgata tgatgctcgt cgatcatacc ggggcgctga cgtttggcga
    10621 gatcatcacc tctcctgccg acttaaaaga gttcgaggcg gagattcgct acaagaaaca
    10681 actcttcggc tacctctacg gacagccgac cgtgccgttc ctcctcatct cctcggtcga
    10741 catatcccgg acggccgtcg tccgccgcct tctgcgggag cccgacaacg ctctcctcac
    10801 cacccccacc tgcgaggagt tgaagtcact gatccgtccc ggggacctga aggggagcct
    10861 gacccaccag gtccggcaca agaaactggt ctcgatcgcc gatattcccc cgcggcggcc
    10921 gttcgactac aaaaaactgc acgatgagcg gatgcagagc atcatcacct ccgtcacctc
    10981 ccggcgaggc atcggggagc tcgggacgcc ggacgagatc ccgccgatcg tgaagaaagt
    11041 cctctttggg gggctctacc cctccgccct ccggatgctt gacgcccggt accctatccg
    11101 catcaagggg aagacctacg acccggatac gatccagaag cagttctcga aggtcatcct
    11161 ggcggtgaac atcccggagt accggccgat catctatctc cggacgagga acaagaagga
    11221 gtacctcaag atggtgccca atcgggcggg tgggttcaag ttcgagagcc ggcggacacc
    11281 ccacatggcc gggttcttcc tctggctcga gtccgtccag ccgtcgctcg gggccgaact
    11341 gacgcgggaa ctcctcgacg cgttccccgc gatccacgtt cccgaagcgg ccgcggttgg
    11401 aaaaccataa gcatcccggc cccctacgcc cccatggcat cgggggagcg aggcccctgt
    11461 tgctcgcgaa gaccgacccc ggcaggtgag gcatgacgga cattcagatc ggcctgatcc
    11521 aggcgctcca gacccaggcg gcatggctcg agacgccgat gcagttcttc tcattcctcg
    11581 ggcagccgga gttctacctg ctcgtcatcc cgatcctcta ctggtgctgg gacccgcgcc
    11641 tcgggctccg tctcgggctc ctcatgggga tctcgggcgg gctcaacgag gcgctcaagg
    11701 tcgccttcca cctcccccgc ccctactggg tgagcccgga ggtcagggca ctcgggaatt
    11761 acccgtcgtt cgggctgccg tcggcgcatg ctcaagggtc gatggcgctc tgggggctga
    11821 ttgccatcga tgcccggcgc tggtggttct ctgcctttgc ggtcgctctg atcttcctca
    11881 tcggcgcgtc aagggtcgtt ctcggtgtcc acttcccgac cgacgtcatc gcgggctggg
    11941 cgatcggcgc cgtcgtcctc ctcgggttcc tggctatcga ggagccggtg ggccgccggg
    12001 tcgccgccct cccgctctcc cggcaggtgc tcgtcgcgtt cgcaggttcc ctcgccctgg
    12061 cgctggtctc gtttaccgcc ctcgcggccc tcggggactg ggaggtcccg gcgtcctggg
    12121 cggcgggggc gcttgagagg tcgggggagc ctatccaccc gctcttcctc cgggacgccg
    12181 tcacggcgtc ggggctcttc ttcgggttcg cggccggtgc ggcggcagag caccaccggg
    12241 gaagcatgtg cgtcatcggg aaggggtcgg tccgcctgct ccgctacatc ttcgggatcg
    12301 ccgtgaccgg ggcgatctgg ttcgggctcg ggctcctcgt cccgcctgac cccgatgccg
    12361 ccatctatct cttccagtat ctccgggccg ccgccgccgg cgcctgggtc tcgttcggtg
    12421 ccccggcggt ctttgcccgg tacaacctgg acggggcgta gggcaggaat atatccgcgg
    12481 caggcgagga tcctgtatgg atccggagga aggcggtctc acggcgctcg aaggggcgat
    12541 cctcggcgtc gtgacggtcc tgcttgccct gcttgcagtc cacgccctga ccgttcccgc
    12601 cggtcctccg gcaggcctcg tctactcggc catcggcgag accgggggat gcctggttgt
    12661 ctccggtgac gtctatgggt atgcgctcgc gagcgggagt gtcggcggca ccgatctctg
    12721 gtgcgcccgg cccgaccgat cgaggatggg gtcggttgcc tgcaacgtcc gcctcgttat
    12781 cggcgatatg gggagcgtcg atatgagccg gaccgcggtc gnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnngatc gtggtcgagc gggcggtgcc gccgtggatc
    12961 acgccggtga tggatctcgg gtgaaatcgt ccttcagcag gcttttatag ccgaaagtta
    13021 actcaggttc tatgcttgca gaggggatcg ctctcggctg gattctgatc gtgctcgggg
    13081 cggtgctgct cctggtggag gtctaccagc ccgggttctt catcg
//
LOCUS       DAJB01000136            8503 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_106047, whole genome shotgun sequence.
ACCESSION   DAJB01000136 DAJB01000000
VERSION     DAJB01000136.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_106047"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    782..818
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1514 a         2894 c         2590 g         1468 t
ORIGIN      
        1 ggccaggtcc ggcaccaatc tcatcaggga agaatgacag ccggaatagg gcttcaccag
       61 agcccgataa gggaaatctt cttcccatgt tattttttgt catgaacgcg ggttggtgcg
      121 caacgtaatt cggccggatt ctctgagatc aattatcagg tatatgctgg agcactaccg
      181 agcaggccgg ggggagggtc gtggtcccga agatggcggt gcccgggagg ggctacttcg
      241 ccatctgcga ggataccgag cagaaccggc tcggcctctg gaatgaggat ccctcggcgg
      301 gttgatcagc caaccacggg acgactgccg ccggtgcggc accctctacc cggacaccgc
      361 aaagggctcg aagacgggaa gaccatcctg ccccgccgcc gaccccggcg ggatcctggc
      421 agtccgccgg gctctccgat caggtgggat cacctgctat ccggaaaaac actcccgaaa
      481 agacaccttt acgcttgatg agctcccggg atcattcgga tctcgggacg gacggacata
      541 acgctgctat gaaggattta aacccaggat ttcagttttt cggcagggag aaaagtttat
      601 ttacgccaag aagcatcgtc caccacatga agagcatgtt tggtatcctt gccctcatgg
      661 tgatcgcgag catcatgatc gcagggtgtg tgcagccagc gggtaacgag acagtgaccc
      721 ccggtacgac accgggactc accgagaccc cgatggcaac agagaccccc atgacgaccg
      781 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gaccatggag accgggacca
      841 ccgtaacgcc cggggccaca acaacgaccc ttgccgcggg cacgacggag accccgccgg
      901 gacctccggc ggcgctcacc gctgacgaaa ttgtctccat caccgatagc gggttcgtgc
      961 cgaacacgat caccgtcccc gtgggcacca cggttggctg gaccaacgac gccgcgtcga
     1021 accataccgt atcggcgacc ggagcagccg gattctttga ttcaggacta ctccagccgg
     1081 gagaatcgta cagttacacc ttcaacggcg cgggcaacta tacctaccag tcgttgacga
     1141 ccgggattgc aggtgaagtc atcgtcaccg cctgaccctc ctttttccca ccggcggttg
     1201 taagggagaa cccttacgat cgccggtatg ccgcggcggc ccggaccagt gtcccgctaa
     1261 acccctccga aaagtaggca tgggtatatt gaccgaccgc attctgcgcg gcaagcccgt
     1321 cccggccgcc gtcgatcccg cggccccgga ggagttccag cacaaaccgt gcgtccgagg
     1381 cgcagtcgag gcgtgagtag tggaattcgt ggccccggaa ggcggagccc ggtgcaagga
     1441 ccggggcccc gccgaccacc cgggcctcca cgtagccgag accctggatg cggtccgtca
     1501 tctccgtcgt cgccgggagg acacccgcca tcgggtagtc gccttccccg gtcacgagcc
     1561 gctccgtgag gtaggcgagc ccgccgcact cggcatatac cggcatgccg tcgtcgaccg
     1621 cccggtgaac gtcctcccgg caccgggagg cagaaagcgc ctccgcgtgg agctccgggt
     1681 agcctccccc gaggtagagg gcgtccacgt ccgggagccg atccgtcatc gccgagaaga
     1741 agaccaggtc cgccccggcc catgcgagcc tatcaaggtt gtcggcataa taaaagcaga
     1801 acgccgcatc gcgggcgacg ccgacccggg cgcctacctc cgatcggtcc ggaacctccg
     1861 gcagcgccgg gaggggtggg gccgaccggg caaggccgat gatgccatcg aggtcgcacg
     1921 tctcctcaac caccgcaccg aaccctgcca tggccccggt ctcggccgcc atcgcgaggc
     1981 cgaggtgccg gctttcgacc gcaaggtctt tgcgccgcgg gattcccccg tagaccggga
     2041 ggctttttgt ctcctcgatc atggccatat ggcgaggact cccgatccgg ttgaagatag
     2101 tcccggcgac ccgcaccccg ggatcgaacc ccgcataccc ccggaccatg gcatggacgc
     2161 tccgcgacgc gccccctgcg tccaccacga gaaggaccgg agcgtcgaga accttcgcga
     2221 cgtgcgccgt gctcgcgata tcggtccctt caagtccatc atagagcccc atcgcaccct
     2281 cgacgacggc gatgtcggcc ccggccgagg cccggacgaa cgtctcccgc acgcctgcct
     2341 cccccatcat gaacgggtcc aggttgcgcg atctccggcc gcagatggcc gagtggtgcg
     2401 tgggatcgat gaagtccggg cccaccttga acggctggac ggcgagcccc cgggcagcga
     2461 gcgctgccat caggccgctt gcaagcgtgg ttttgccgca gccgctgtgg gttccggcga
     2521 cgattactcg tggaatcgag cgcataacgt tcctgtagtg ttcaataccg caggagataa
     2581 atatgacctg ggataacctc gatatatcaa ataaatttgc cttaccgcaa gcgggtttga
     2641 cctgctcgcc ggcacggcgc tccacaggtg aaatatcatg cacatcatgg aaggattctt
     2701 accaagcccc tggtggcaga tctggtttct cgtctccctg cccttcatca tcatcggact
     2761 gtatcaactc aacagacttg tacaggaaaa acgggaagtc ctgccgctcc ttgcggtcgc
     2821 aggcgccttc gtcttcgtcc tctcgtcgct caaactccct tcgttcaacg gaagctgctc
     2881 ccaccccacg gggacggggc ttggcggcgt cctcttcggc ccctgcatcg ccgccgtcct
     2941 cgggctgatc gtcctcgtct tccaggcggt cttcctcgcg cacggtggga tcaccaccct
     3001 cggggcgaac gtcttctcga tggggatcgc ggggccggcc gttgcctact tcatctataa
     3061 ggccgggacc cgggcgggcg caaacaccta cgcaacggtc ttttgtgccg cagccctcgc
     3121 cgacctcgtc acctacgtcg tcacctcggt ccagctcgcc ctcgcctttc cgggaaccgc
     3181 cgggttcctc ggggcattca cggccttcgc cgcgatcttc gccgtcaccc aggtcccgct
     3241 cgccatcgtc gagggtgcgg tcatcatgct ggtcttcaag tacatcgtcc agctcaaacc
     3301 cgagatcctc acccgcttaa acctcctctc cgagagcacg attcggaaac tccaggaggc
     3361 caccgcatga aatacggcat ggaacttgcg gtcgtcgcgc tcatcattgt ctttgccgcc
     3421 gtcttcctca tccaggacgc cgcgatccgg gcctccgggg aggaagcctg gggtggggcg
     3481 gacggtgagg ccgccgacct catcgaggcc tccggctacg agccctgggc cgagcccttc
     3541 tgggcacccc cgagcggtga ggttgagtcg ctcctctttg ccctgcaggc tgccatcggc
     3601 gccgtgatca tcggctacgt cttcggctac tggcagggca gcagaaaaac cgcctgattt
     3661 ttcaggtaat cctgcaaacc ggataggaag acgcgcatgt acgaggtgct cgaagacttt
     3721 gcccagacta acggcctccg ggagacgagc ccgggcctca aactctttat cgggctatca
     3781 agcatcctcc tctgcgtctc gtcgcccgat cctgccgccc ccctgctcgt cgcggccacg
     3841 atgagcgccg cggtcctcac cctcgcaaag atcccggcac ggttctacgc ccggctcctc
     3901 ctcgtcccgg tctcgtttgc gatgatgagc atcgctgtgg tcctcttcgt caccgggagc
     3961 gggccggtcc tcttcgaggt ccccggcctc cccctcgcgg tcacggcgga cggcgcgaac
     4021 ctcgccctcc tcctcctcgc ccgggtcttt gggggtatgt gctcgctcta cttcatcgcg
     4081 ctcacgacac cgatggccga ggtcttcgat atccttcggc gtctccgggt gcccgccgtc
     4141 ttcatcgatc tcgccatgct cacctaccgg ttcatcttca tcctcatcga ggaggccggc
     4201 cagatctacc gctcccaggt gatgcgcctc ggctacggcc ggttccggga gtcggtgcag
     4261 tcgtccggga tgcttgcagg agcgctcttc ctcaggacct gggagagcgg cgaagccctg
     4321 gtcctcgcaa tggacgcacg ctgctacgac ggcaaactcg gcatccccgg agaaacccgg
     4381 cccacctcct ttatcgcgct cgtatcgacc ctgctttacc tcgggaccat cttctgcgtc
     4441 tcgctttcca ccggaggtcc ggtattcgca tgacagcacc gctgcttgaa accgacaacg
     4501 tcacctacac ctacccaaac ggccctgccg ccctcgccgg ggtgagcgtc cggatagccg
     4561 ccggttcgaa gaccgccctc gtcggcccga acggcgccgg gaagtcgacg ctcctgttga
     4621 tgctcaacgg catgctccgg cccgcctcgg gcgaggtccg ctttgacggc cggccgctcg
     4681 cctacgacaa ccggagtctc cgggacctcc gccgccgggt cgggttcgtc ttccagaacc
     4741 ccgacgtcca gatcatcgcc ccgactgtcg agcaggacgt ggccttcggg ccggtgaacc
     4801 tcggcctctc tcccgatgcc gtccggcggg cggtccggga cgcgctcggc tacgtcgggc
     4861 tccggggcta cgagaagcgg ccgcctcacc acctctccgg cggtgagaag aaacgggtcg
     4921 ctatcgccgg catcctcgcg atggagccgg cggtcctcgt cttcgatgaa ccaacgaaca
     4981 ccctcgatcc cgcaagctcg gaggaggtga tggaactcct cgacgagctc gcctccggcg
     5041 gcaggacggt gctcatatcc acccacgacg tcgaactcgc ctaccgctgg gccgactccg
     5101 tcatcctcat ggaacggggg ggcgtcctcg cccgggggcc gccggaagag gtcttctcgg
     5161 accacggcct cctcgccgcc gcccgcctca aaccgcccgc cctcctcgac ctctacaacg
     5221 agcttggtct ccggggcgtc atcgaccgcg gcgtcccccc aaaaagcgtg ctcgagttca
     5281 ccgaccggat cgagcgggag atgcacggcc acgccccggc ccgggacgag cccgggaaga
     5341 tctacctctg caacgccggc cggaccggcg gcgaagagct gcgggtgctc gttgagcagg
     5401 gggtggtcga gcacgtcggg gcgatgggca cccgcgccaa ggagttcgcc aacagggagc
     5461 ggatcctcct cgactacacc tacggcgtca tcgacaagtg catcttaaaa gccctcatcg
     5521 gggagaactc gctcatcatc accacagggg gcatgatcga gcacgtccac cgccgtatcg
     5581 gggagtacag cgccgagagc gggcgggcgc tcgcggcaac cccggtgcgg gggccgggga
     5641 gccccggacc cggccgggag agcatcccgg attgatggaa acgcaccagc cccctgacag
     5701 gcacctggtc cttccataac tttttttccc gggccgcccg agacctcccc gggcgaagat
     5761 taatccggtg ccggtacccg aagggtacct atgatggtcg aaaaggtgct gcagcggatc
     5821 tcggagcagg tgaaacggaa gcggatgagc ctgcctgacc acaaaacaaa gattgtctgt
     5881 accatcgggc ccgcatcccg ctcccgcgag gtgctcgccc ggatgatcct cgcaggcatg
     5941 aacgtcgccc ggctcaacct ctcccacgga tcgttcgagg agcatcggga gaacatcaga
     6001 aatatccggg ccgcggccga ggatgtcggg cttcccgtca cgatcctgat cgacctcccg
     6061 ggaccaaaga tccggatcgg cgacctcgcg aaagagccca tggaactccg caagggcgag
     6121 accgtcaccc tcacgaccgc cccggtgccg ggtgacccat cccggatccc tgtcgacttc
     6181 gagcagttcc cgcaactggt cgcggagggg agcatcatct acctcagcga cgggttcctc
     6241 cagctccggg tcgacgaggt cacgggccgg gacgtccggg cgacggtggt catcggcggc
     6301 cccctcctct cgcgcaaggg gatgagcctt gtgggcggcg gggggattct cgcggtcagc
     6361 gccgtgaccg accgggacct cgagtgcatt gagttcgggc ttgctgaggg gcttgacacc
     6421 ttcagcatat ccttcatcga gcgggcggag gatatcctga aggcccggga gtatgcggcc
     6481 cggtccggga agaccatccg ggtgatagcg aagatcgagc ggcaggaggc gcttgcacac
     6541 ctcgatgaga tcctccggga gaccgacggg gtcatgattg cccggggcga cctcggggtc
     6601 cagatcccca tcgaggaggt tccctcggcg cagaagcgga tcatccagaa ggccaacatc
     6661 ctcgggcggc cggtgatcac cgccacccag atgctgatgt cgatgaccga taacatccgg
     6721 cccacccggg cggaggtgac cgacgtcgcg aacgccatcc tcgacgggac ggatgccgtc
     6781 atgctctcgg aggagacgtc catcgggaac taccctgtcg agacggtcgc gatgatggcg
     6841 aagatcgccc gctcgaccga ggggacgcgc acaagcgtcg gggcagggtg cagcccgctc
     6901 gcctacttcc ggcagggaaa ggggcgggag aatatcaccg tcaacgacgt cgtctcgcta
     6961 aacgtcatcg aggcgctgga tgcacttggc atccggttcg tgctgacgcc cacccgctcc
     7021 gggagcaccc cccggaatat ctcccggttc aaacccgagg cctggatcct cgcgttcacc
     7081 cggaacccga gcaccctcaa gtttctggcg ttctcctacg gggtctgccc gttcctgatc
     7141 cggagcgagg aggagtactg gcacggggcg atcctcggct cgatcaggga ctcagggctt
     7201 atcgagcccg gcgaacgtgt ggtgctcacc gagggggtat cccccggaca cgaagggacc
     7261 gactcgctca tcatcctcac ggtcgattga ggggagacag cctcgggacg aaaaacccta
     7321 tcccggtgga ggacgaacac tccccaatca tgggagtgat cgttccggga gaacgggatg
     7381 cttcgggcca ggacggcgtg ttaaaggaga agaccgcacg cctctcggtc gcatcgaaca
     7441 cgtttcttgt cgtaacaaaa ctggccgtcg gcgttgccat cggatcggtc ggtatcatat
     7501 ccgaggcgat ccactccggc atcgacctga tagccgcggt catcgcctac ttctcggtcc
     7561 gccgctcggc ccagcccccc gacgagtgcc acaccttcgg gcacgggaag tacgagagca
     7621 tatccggcct cctcgaagcg gtccttatcc tcgtggccgc cgtcctgatc atcaacgagg
     7681 cggtgagaaa cctcctcggt ggagaggaga cgctgaacgt cgaggcgctg ggattcggca
     7741 tcgccgtcat gctgctttcg gccggggtaa atttctacgt ctcttcccgg ctgatgacgg
     7801 tggcgaagaa gaccgagtcg atcgcgctcg agagcgatgc ctggcacctg cgcacagacg
     7861 tctacacctc cgcaggggtc gtgggcggtc tcgtcctgat ccggctcacc ggacttacga
     7921 tcctcgactc cttcgtcgcg ctcggggtcg ccgtcatcat cctgaaggcg gcattcgatc
     7981 tcatccggcg ctcgttcgag gacctcgtcg accggagcct cccccccgag gaggagggcc
     8041 ggatccggga gatcatcggt gagcactgca cggacgtcat cggtttccac cggctccgga
     8101 cccgccggtc ggggccgaac cggttcgtgg acctccacct ggtcgtcccg aagaccgcga
     8161 cccttgagga ggcttacggt atcgtgaagc acgtggagac cgatgtcaaa ggggagttcc
     8221 cccgcacgag cgtcaccatc cgggtgcagc cctgcaccgg cgaggactgt tccgcctgcg
     8281 gggtcttcac cacctgcccg gagtgcgacc acctgacgct ccacgatggg ttagaagagc
     8341 ggcaataaga ggttgggggt tcctaccccc aggaaaagag gccccgggcc cgcatctcct
     8401 cttctgcgag tgccgagagc cggtcctgct cctgctctat cgtcctgacc tgctcccgga
     8461 gcatcgtctt ggtctctgca tcgcaggtgc agttctcgat cga
//
LOCUS       DAJB01000137            3119 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_177446, whole genome shotgun sequence.
ACCESSION   DAJB01000137 DAJB01000000
VERSION     DAJB01000137.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_177446"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          697 a          917 c          997 g          508 t
ORIGIN      
        1 ccgggctgtg tctccggtgg cccctcggtg aacgagtagg gattgttgtc catggtcatg
       61 gcgatcttcg ggttggtgac agccgttgca gccttctgat aattgacgtt cggggagagc
      121 agacagatca gggacgatgc agccgcccgc ctctcccagt atgactgcat ctcctgctca
      181 tactgcttga actcctccgg attggcattc atcgaggtgt aaagagcacc ctgggccccg
      241 gcaggctgtt cggggagcgg tccggcaatc atctcggcgg ccgtccaccc gaagatcggt
      301 atggccgagg agaggagcag aaagattacg agagtggaga tgagggcggt tccgctgtcc
      361 ggcgcgacgg tggacatcag gagagccagc gcgaagtagg tcaaaacgaa tatcgccgaa
      421 accccggtga acaccaggat tgcacccagt tcacccatgg tgggtacgat gcccattata
      481 aggaggatgg cgaccgatac cgcgatcgtc gcaccgaggg caatcgccag tgccagggca
      541 ccgccgagtg cttttccgtt cacaacctcg tccccgaaca ccgggtggga aaggagagtt
      601 ttgagcgatc gggtctcctt ttccttcgat ataaggtcaa atcccattgc gatgccgagg
      661 aacaccccaa acatcacgaa gtaatcggct atcttcgtaa atataagcag gggtgataac
      721 ttttcgggca tatatctcgt aacctgagtg tcggaagaga cggactgcat ctgttgtgca
      781 tacgacgcaa gcatatcgct gtaccggtcc gctccgtcgt ggacgccgat cgcggagatg
      841 atcaggaaca gcgcaaatat gctgaggaag cgccggtcgg tcaactggtc gacgaactcc
      901 ttctgcagca ccacgaacag gcgacctgca gtcacgagaa gccacccccg gtggagggga
      961 gaggaggcag gacgtcgcta taccgccgct tgccgacggg gtctgtcgac accgtgacac
     1021 cgtcacccgt atgtcccatc ccaggtggca gggcccgggt tgtcccggca acccggcata
     1081 ttactggaga acacctgatt gttgtcattt ttcatcccaa atccgattgt taccgcaggt
     1141 cgagccgcat gaaccgcacg taagcgacgg cgagaaagat cgccgggaac gcaacgaggc
     1201 cgacgatatt ctgccagaat tggcccagcg cctccgccag gtccggcgtt cccgtaaggg
     1261 acccggtcgt cgcgtccccg ccgtggtggg gatcgaccag catccctgtt atcgtctcat
     1321 agttgccgtt ggggctgagg gccataaccg aatttttgac ccagttatac gtcgcgtagt
     1381 agtcgctccg ctcctccagg taccgctgga tctcgtcagg cagggtgtca tcggggggcc
     1441 attcaggcag agaaccggcg gctgaaccgg ccgcatatga tgcggcatgg ggcacaaccg
     1501 tcgagaagag gatgaagacc gccatcgccc aggcaagcgc ccggccgccg tcttcgctga
     1561 acgcggagaa cgcgatggcg acgccgaagt acgcgagcag gtagagggcc gtggcgcccc
     1621 agcagagcat cacgatcact gtatcgtaag gcgtaacagt gagcccggcg acgagcaccg
     1681 cacccagcgc caggaggtac ggcacgacgg atgcgagcac cagcacggcg gtgccgccca
     1741 cgaccttgcc ggtgatgact tcgtcgcgat agaccggccg ggaaaggagc atccgaagcg
     1801 accgggtcga acgctccccg gagatgaggt cgaagcctgt ggcgatcgcg agaaccggcc
     1861 cgaagaggag gagctgatcc cccacgctca ggaagatcca cagcaccgac gggaggacaa
     1921 aggaggaaga ggtagtgagc ggtgatgtcg acacggtatc gacgttcccc gccctctcca
     1981 gaagaaactc ataccactgc agcgcactcg cgtaactggc aagaccgcgc tccagggcaa
     2041 ggaaggtcag gatgccgaag agcgcgagca ccagaaggaa cgtccggtcg gtgaggtggt
     2101 cggcgaactc ctttcgtgcg atgatcgcca cacgggagag gtcgatcttc attggcacat
     2161 caccggacat ccatcctctg gaacgccata taggcgagca taaagagcac gagcggtgcc
     2221 atgaagagca ccagcgtgtt ctgccagaac cgtgcgaggg cgtccgccgg gcgtgccgtc
     2281 ccgtcgtcgg ggaggaggac tacggaaccc tcaatgaacg aataggtggt aagccgcggt
     2341 tcaagagcac tctttgcaac cagttcgtag ttccactgcg gtgagaagac cgtcgcgacg
     2401 ctgttcacca gtgcgagccg tgtctggtag tcttcccgtt cggaggggct cgcacccaga
     2461 gggtgagcac cggcaacgac gtccttcgca accgctccca ccgtcgggac gagggcggaa
     2521 aagatgaaga acgcgatcag cgcgtacaaa aatgcctttc cggtccgggg caccaccgtc
     2581 gagatcgcca gggcgagact gaagtagaac gccagaaacg caaccgttgc gatcccgaag
     2641 agagagatcg agaccaggtc gtccgggccg ggcacgatcc cttcgatgag aaggactgcc
     2701 agcgaaacgg caagccccgc ggcaatgagc gccgtcaggg cgagaaggcc acccagcgcc
     2761 ttgccggtga cgatctcgtc ccggaagatc ggccggacga ggagcaactt gagcgaaccg
     2821 gtctgccgct cccgcgagac gagatcgaac cccatggcga tcgcaaggat tgccccgacg
     2881 gtagagagtt ctccggggag gcgggcgtaa acgtccgcgg cgttcggaag actgctaccg
     2941 aactcttcga caccccatgg ggtgacgacg ggaccggcag catacgccgc ctgagtcgcc
     3001 tggtatccgg taagaccctg ctggaaaggg aggatgcaga ccacgacgag caggaccgca
     3061 aagacgagga acgtccggct cgtcaggtgg tcggtaagtt ccttacgggc caccgtcag
//
LOCUS       DAJB01000138            8669 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_66850, whole genome shotgun sequence.
ACCESSION   DAJB01000138 DAJB01000000
VERSION     DAJB01000138.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_66850"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1667 a         2701 c         2710 g         1591 t
ORIGIN      
        1 tagcaataaa taagccattt agaacagtcc ttttacctgc agaacttctc tcacagattc
       61 tggaggaaat cggactatga agaacagatc tcttgtacga atcattggtc ttggcatgat
      121 cctgacgctc atgctcatgc cggtatcgcc ggccctcgca gcggggttcg aattaccctc
      181 cccggcgagc gcaacgactg ttaaagccat cgagaggccg gatatgacgg ctgtctcctc
      241 cgccctctgc gacgccttcg ggatcgaatg cgacagggcc acgatggcaa aaggcgtcta
      301 catcgacccc actgtctggg aattctcgcc gcaatggacg ctgatcatgc tcggttcgac
      361 gaaacagcag gaaaacatcc tgaagttcgt cgacgggctc gatgcctcga aagagaagaa
      421 ggccgtctgg cgatcgagcc ttgaagaact ctggaagaag tacccggtcc gggccgtgga
      481 gacggaggac ggccttgcgc tgaccatcgt ccccgagaag agcgatgtgt ggcttgcagt
      541 gtccgaaaac tcggctctcc gggagctcga tgaggagatc ggccgtgcga tggcatcgag
      601 catggacggt gaagtcggcg tgcggtggaa cggaggcact catagatgga taatcgaggc
      661 ggcatgcgtg cataactcag ttaatgagac aaatgcaggt atagcaggag aacatgcata
      721 tgaaccagac tggtggtatg aaggttcgga tcttgagata attctccagc gctgctacaa
      781 ccacggctac gtccctccca cacccctccc tcctgttccc ggtgtcccgg agggtttcgg
      841 cagggccccc gagaattgcc agatgaacgc gacggatgcg gcgaccgcct actacaataa
      901 ccgcctcgac gacgctttca cgtctctcgg gcattcgagc cacttcatga ccgatatcgg
      961 taacccgatg cacacgggaa acctcttcgt gcagggctcc ctgactgtca tggggtggaa
     1021 tgttcacggt gcgtatgagg actacgtaga aggtaactgg gaaaccctcg ggttcaacgc
     1081 gaccgtctat gacacggtag aaacggaagt ctggagtaac cccgattatt cgacacggct
     1141 tctcggctgg aagacccagt gggtccgcga ccccctcatc ctcctggtca ccctgaacta
     1201 tctaactcac ggcggagagt tccacctcga agagagcccc gcaatccgtg aaatcacgct
     1261 atggagcctc ggcgagacga cgaagcacac caacggcctc gtgtactacg tcatcaagga
     1321 cggcatccgc acccacgtca tcaccgcaac cgcgggcccg cacggctcca tctcgccctc
     1381 gggcgacgtc caggttctct acggtgagga gcaatcgttt gagattactt ccgatagcgg
     1441 ctataccgtg gacaacgtcc gcgtggacgg cgaatccgtc ggagcccgca cgacctacac
     1501 cttcgatgcg gtagacgcgg atcacaccat tgaggcgacc ttcaaagaaa tcaccggctc
     1561 cggcaacgaa tgggtctggt cgagggacgg atgggatgga tggtcccacg aggcgtcgtg
     1621 gtcgggaaca cagaccggac cctgctccga atacggcccg gtgattgtcg acgaccacgg
     1681 cgagcacggc atcgatgtaa cccttcttgc cggttcgacg accgctcgtg tcgagcggca
     1741 gttcacggat gcttcgggaa ccgggtggaa taccctcaca ttcgtcgggc tcctgacggc
     1801 gtcggacgta cccggcggac ggtggatgac gatcgaggtg aacggccagc aggtctttgc
     1861 aggcaccgcc tcgcagacac ccccgggcaa cggccagctg ttcgagatca gcgtcccgtt
     1921 cccgcagtcg gacgaggtga acgtcaggat ctcccacggg cagagcccgg cctggaggac
     1981 gcggtttgca atgcactgct actccctgaa cctgacgctg gacaacaacc agagaggcgt
     2041 cgctgtggaa gacgcaccct tcgtcatacc ggaccccgcc tcgctggtgg tcaacgggac
     2101 cgccgggagc ccctgagccc tgtcgctccc gcctgcgggc ggtgacctgc actccctttt
     2161 tttcaagacc accctgtgaa gatcacgccc cctcggatgt acctgatgac ctggtcattc
     2221 cccgtctttt atatccccgc accgccatga tcttgtagac cgatgcaaaa caaccagcgt
     2281 acgccgtgcg aaaacccgct ctggccctgt gcgatgaccg gggcggtcgc ctgccttgcc
     2341 gggttcgagg acctcgccgt cgtcgtccac ggttcgagcg gctgctactt ctaccccgca
     2401 tcgctcgtcg gggtccccat ttacgggacg ttcctcgtcg agaacgagat catcttcggc
     2461 acggagtccc gtctcgcgga ggtgatccgg gaactcgagg gccggtaccc ccggatagcc
     2521 gtggtcaaca cctgcgtgcc cgccatcatg ggcgaggata tcggtgatct cgcgcgtgac
     2581 gggcaggtcc tcgtcgtcga cagcccgggc ttcctcgggg acttcgaggc cggctatcac
     2641 cgggcgctcg gcgaggtcgc gccggaggtg gaccccgatg ccgccggcgt caacatcgac
     2701 ggcatctgcc gcaccgaccc cttctgccgg ggcaatgcca tcgaggcgcg gcgcctgctc
     2761 aacctggccg gcgccgcagt cgccaccacg ttctgcctgg accgctacgc tgcggctcac
     2821 cgtgcggcgc ccctcaccgt cggcacgaac ccggacctcg cgagcggcgt cgggacggcc
     2881 tgcggctcgc tcctgggcct cgatgccgtt gccgggacgt tcgagagact tgccgccgag
     2941 atcgacggcg tcgatgcacg cccggtcacg gcagagatcg catgggccga ggcccggatc
     3001 aagaaggcct gcgacaagta tctcaggagg ttcgacccgc cccgggtggc gatctctgcc
     3061 gggtactcgt atgccacgtt tgccgccgaa ctcctcgacc ggtacctcgg tgccgatatc
     3121 gcctgcatcg ccgcccggaa cgatccgggt gtctgcaacg atgccgtcac ctctacgacc
     3181 gacttctcgg tcatccgggg gatgatccgg gacgccgaac cggacctgat cctcggctcc
     3241 tcctacgagc gggggatctc ccccggcgcc gcattcgtcg ggttgacgcc gccgatccgg
     3301 ggccgggtcc tcctctcctc ccgggcagtg gtcgggatcg aaggagcgct ctggctgatg
     3361 gatgaggcgc tcaatgcctg catcaacaaa aaccggcgcc cggagggcga gcagatggac
     3421 ctctcctgag caaagcgtta ttacgcccgg tgtcagagca ctcctgcagg aggacggggt
     3481 tcgcccggat ccccgctctg ccgctggaga aatgtccaca tgccgctcgt caagatcgat
     3541 atccgctccg gaaagcctgt tgcttaccgg caattgctta tcgaaagcct ccgtgaggct
     3601 tttgtagaca ccctgcagac cccggaggcc tccctctgga tccggctctg cgagcgcaaa
     3661 gttgagaatt tcccgatgcc ccccggccgt tccggcgacg cagtcctcat cgaggtctcg
     3721 ctcatgccgg ggcgcgagcc cgcgacgaaa gaggggctct atcgcgcgat cgtggagcac
     3781 ctggtctgtg atgcgggagt tcgccccgac gacgtctgta tcgtcctcta cgagccgccg
     3841 gccgagaact gggccgaaca cggcggagca ttcatggcct cccggtgagc agccgcaccc
     3901 gccataagat atttatggcc ccgtgagcac agggtgccgg cctgtccctg tgggcagacc
     3961 ggtatgaggg ggttatgata tgacacacta cggcggacag ggcgcccacg gcatgctcct
     4021 tgagaagatg cgcgatcttc ttgacgagga ccagatgcgg cagctgacga tacggatgat
     4081 cgagtcccgc atcaagatga agcagcagca catcgagatg atgaagtaca aggtcgagac
     4141 gtacgagatg gcacgcgaca tgctcaaagc gggcaagaag tgagcctgtg gatcgagggt
     4201 gctccgggat gcaagcggcc cggggccgct gcctattttt agccttcacg acgaatacac
     4261 cggtatggac ctggtaatca gggggtatcg cgaacaggat tttcgttcgg tctctgccct
     4321 cgagcgggag aacagcccgg gggactgcaa gcccgaggtc ttcatccggc aggccggcgt
     4381 ccttttcagc gatacgtttc tcatcgggga atgcagcggg aagatcgtcg gctacaccat
     4441 cggggctctc gtgcagcatc agttggggac gggatggatc gtccggctgg tggtggcgga
     4501 gcggtaccgg cgccgtgggt ttgggggaga tctcgttgcc gccgtcaccg gcagcctccg
     4561 ggagcggggg gcgggggagg tctacctctc ggtcgcgccg caaaaccggt ccgcccgtgc
     4621 actctacgaa aagcacggtt ttcgggagac ggatttttgc ccggcctact tcggtgaggg
     4681 cgccgatcgc cacatcctcc ggagggatct cgccggcccg gaaggcttaa tcagtccgga
     4741 ctgagattgg tgtgatacta tgtccctcac cccgctccgc ataggcgagg cgttcaaacc
     4801 ggtgccgcgg ttccggtcgt acctctacgc gaccctggtc ctgaccgtcg tcgtcctcat
     4861 cctgccctgg ctgattcccg tcgtcgtctt cacctggggc ttcgtcgctc tggccatcgc
     4921 actacccgtc cttgccgtca tcgtcttcgt cgcctactgg atacccctct actacgagag
     4981 tattacctac cggctcaccg tgaccgagat cacctggcag cggggagtct ggttccgcca
     5041 gacggggatc gtcccctaca accggatcac gaacgtcgat atcgcccagg gccccctgat
     5101 gcggttcttc tccttctcgg cggtccgggt gcagaccgcc ggctactcag cccaggcaca
     5161 ggctgagatc ctcctcaacg ggattgcgga cccaaaagac ctccaggaga agatcatgaa
     5221 cttcgtgcgg aggaccactc ccgtcgcggt cgggggcgag ccggaacaac tgccggccgc
     5281 cgacgcgacg gtggaagaac tgcgggcgat ccggaagctg ctggagagcc tggtggagaa
     5341 ataaagcctt tatttttgct gcaaaaaagg gggaattatc tcccggcggt caggtcccgc
     5401 ttgatgacca ggaccggctg atgggcgttc ttgatcacgt actccgagac cgtcccgagc
     5461 aggcggtcgc ggaggttcga tctcccgtgg gaaccgatga cgatgagcga gaccccttct
     5521 gcgtccgctg cagccacgac ctcctttccc gggctgccca cgggcgtccg gacggtcacc
     5581 ttcacgccct gcttctcgag cgcccgccgg gtatcgagga tccggtgctc gatctcctgg
     5641 cgcaactcac tctgggtctc ggtctcctgg tcgggcacgg tcccgaggaa cccctgcccc
     5701 cccgaggcga tcagcgtgac gtcctttgag tcgatgacgt ggacaaggac cacctcccgg
     5761 gttcccgcct ccttcagtgc ggggatgtag cccagcacct tcatcgaggg ctctgaaaag
     5821 tccgttggaa agacgatgcg ttcgaacatg ctctggcact cactccgatg ttctctctgt
     5881 gggtcggata tttaatagat gtcatttaga acctacaggc gagctgggtc tcggtccact
     5941 gcaccgggtt cttcgttgtc ctgtggttcg catgcagaac aaactctccg gattctcctc
     6001 gtttagcaga gttgtaaatc tcctgaccga gagtgttctt cgggagatgg ggctgaacgg
     6061 aacggcaggt gctcgcggtc aaccagctca agacgcaggg gagcatcacc aacagcggtt
     6121 atcagtatct caccggcgtt gcggcgacga ccgcgctcaa ggaactcaag gttctggtcg
     6181 accttggcat tctggagcgg agggcaccgt cgaggaagaa gacacagtat gtcctgcgcg
     6241 aggaacggcc atgatgccgg gtgtggataa tagcacggag tttcggcaga atgggcgccg
     6301 ctcctgctct ggagcagtgc ggatgccctg ccgggtgcgg ataacgtgcg gatatatggt
     6361 aaccctggag cgagatgagg gcgcgatcga ccagcattag attcttcttc attgccggga
     6421 gaatactcag aaagaaatca gaggcatata gaggggagac atggcagtcg aaacaatcag
     6481 agctcggact cgtctgggaa catctataag agctgcggca tgacccccgt ccgaatcttc
     6541 atcagcagcg tgcagaagga gtttgccggg gagcgggcgg ccctccgcga ctacctgcga
     6601 ggcgatgcac tgatgcggcg gttcttcgag gtcttcctct tcgaggacgt cccggccgcc
     6661 gatcgccgca cggacgacct gtacctcgac gaggtccggc gctgcgatat ctacgtcggg
     6721 ctgttcggga acgactacgg ctctgaaaac accgagggtc tctcgccgac cgagcgggag
     6781 ttcgatctcg ccaccgccga agggaagtac cggctcatct acgtcagggg cacaaacgac
     6841 gaggcccgcc acccaaagat gcgtgctctc gtcggcagag cacaggcggg tctgatccgg
     6901 aagcggttca gcactccctc agaactggtc accggattgt atgcggcgct agtcgagtac
     6961 ctggtggaga agcagctcat tcgctccggc cccttcgacg cggccccgtg cacgaaggcg
     7021 acgcccgatg acctggatcc tgagcgtatg gcgtggttcc tccgcacggc cagagcgacc
     7081 cgccggttcc ccctcgccgg ggatgcttct cccgctgaac tgctggagca cctgaacctg
     7141 ctcgatgacg gaaaggtgac aaacgcggcg gtcctcctct ttggacaaaa gccgcagcgg
     7201 ttcctgatct cctccgagat caagtgcgcc cacttccacg gcaccgaagt cgccaaaccg
     7261 atcccgtcct accaggtcta caagggcacg gtcttcgatc ttgtggacgc ggcggtggat
     7321 tttgtcttga gcaagatcgc cctctcggtc ggcacccgcg aggccggccc gcaggcgccg
     7381 gtgcgctatg agatccccaa agaggtggtg gcagaggcga tcgtcaatgc ggtcgcccac
     7441 cgtgactaca cgagcaacgg cagcgtgcag gtgatgctct ttgcagaccg cctggagatc
     7501 tggaacccgg gcaggctccc gccgtccctg accctcgaaa aactccggca ggcccacggg
     7561 tcggtgccgg gcaacccgct cctcgccgaa ccgatgtacc tgacgggcta catcgagcgg
     7621 atgggcacgg ggacgcggga catgatccga cactgtgctg aggccgggct gccggagccg
     7681 gagtttgccg tcagtgatgg gtttcagacc atcatccgcc gggtccaggg tagaggtacc
     7741 agcgaaccta gggaaaaacc tagggaagaa actagggaaa aaaccaggga taagatcctg
     7801 gccctaatcg cagccaaccc atctatcagc acgaatgagc tcgcagagca gatcggtatc
     7861 acacccaaag ggattgaatg gcagatccag caactgaagc gggcaggtca gctcaaacgg
     7921 gtaggtccaa agaagggtgg ccgctgggag gttgtcggag atggggatga atagtgccgg
     7981 agaggtggga tgacacactg gacgataacc ctggagcggg agggagccgc ggtcatcgtc
     8041 cggggcgagg gcgacgaccc ccacgctccc gacctgatcg gttacgatct ccgcggcagc
     8101 cccggcccgg tataccggga gctgctggac aggatccgag gcggcgttga ggtgcatctg
     8161 ctcgactggg tgaaggatga gcgcgacctc tcctggattg ccgggtgtgc ctataccggg
     8221 gcgctgctgc accccgggtg gtccgtggag actgatctcc ccctgctgcc ggaggcggag
     8281 gaactcccgg agggggtgat cccgtgaggg aggaggcagc acggcggggc tccggcaacc
     8341 gtcctctacc gcgggctccc ggcaggagac ctctcacctg ctgctcctca ccgtgaaggc
     8401 tcccgtgaac ggccagttgc tcttgaggca gtcggtcgtc cggggcgagg gcactcttca
     8461 acctcgctcc gccgggtgga tagggtagaa gaggttctgg aagaagatgg ctctgtcgac
     8521 gaactgcaca tccttcttcg gactggcggg gttctcttgc acggccggcg cgcacctccc
     8581 gggttccgtt ccctgcaacg atcccgcact gaacctgatc accgccggga cagaggggta
     8641 aatctcagat gtgaactacc ccgccctga
//
LOCUS       DAJB01000139            2649 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_100631, whole genome shotgun sequence.
ACCESSION   DAJB01000139 DAJB01000000
VERSION     DAJB01000139.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_100631"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1878..1893
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          557 a          824 c          757 g          495 t
ORIGIN      
        1 cccccttcac ccccacaaga taaaagaagt ggtatagacg ggcgcaccgg caggaacccc
       61 aaccattcct ggtatttgcg gaggccaaaa gatgccgggg tgcacgggag acccaccatg
      121 gcagcacccc cggccccggg ccccttctcg cccgggagcc ccggagatcg gcatccagta
      181 aaatgacctt catacgctga ataatcggca aactggctgt aaatcgcagg ataacggaca
      241 ggattgcagt ctataattaa atgcgccgca aaatgccgga aaaacaaaat tcccagttaa
      301 tagcgagaat tttcaaaaac acgaaattat aaggcagata aaatagaaat cctgagtcgg
      361 agggttccgt catttttgag aagatatcca gagaagactc aacatcgtta caaccaatcg
      421 tcagtataag gggcctgacc cgggtattcg ggcaggatcc cgagaaggcg ctggcgctgc
      481 accggtccgg ccgctcaaag caggagatct acgaagagat cggtgcaacg ctggccctca
      541 ataacgtctc ttttgaggta atgcggggcg aaacattcgt cctgatgggg ctctcgggga
      601 gcggaaaatc gacgctcctg cgctgcatca accggctcat cgaccctacc gagggcgaaa
      661 tacacataga cggggaagat atcgtcggga tgagccagga gaaactgcga gcgacccggc
      721 ggcgcaagct cgggatgatc ttccagagtt ttgctctcct cccccatcgg acggttctcg
      781 acaacgtcgc cttcggcctt gagatccagg gcgtcccgga ggatgagcgc caccggaaag
      841 ccgaagatgt gctgcagatg gtggggctcg gcggctacgg ggcgagcatg ccctcccacc
      901 tctccggcgg catgaagcag cgggtcgggc ttgcccgggc gctcaccagc gacccggata
      961 tcctcctgat ggatgaggcg ttcagcgctc tcgacccgct tatcaggaga gacatgcagg
     1021 atgaactgct ggatctccag cagcggctgg ctaagacgat catcttcgtc acccacgacc
     1081 tcgacgaggc cctgaaactc ggcgatcgca tcgccctgat gaaagacggt gcgatcgtgc
     1141 agatcggcac ccccgaagag atcctgacga acccggagaa cgcctacgtc gagaagttcg
     1201 tcgccgacgt ggacttaaca cgggttctct cggcaagcga cgtgatgcgc cgccccgaac
     1261 cggttgccca atgcaccgca ggaccaaggg tggccctgca cctcatggag gagcacgaca
     1321 ttccggggat attcgtcgtc acccgccagc gcctgctccg cggccgggtg acgctcgacg
     1381 acgccgccga agccgtcaag aagggaaaac agatgacgga tatcctcatc accgacattc
     1441 ccatcgtggc tcccgatgca tcgcttggcg acatcatctc gctgatcgtc gagagcccct
     1501 accctgtagc ggtggtggac gacacgggca ggctcagagg ggtcatatca cgcggcgcca
     1561 tcctcgccgc tctggcacga aagggggagg agactcatgc agctgcctaa actccccgtg
     1621 ggtgaggccg tcgaggcgct tgtcgaatgg atcgaggagt acttcggctg gctgctcgac
     1681 ggcgtcagcg aagggctgca gttcctcgtg ggcgggttcc aggacctcct tctcttcgta
     1741 ccagcactga tcctgatcct cctcgcgagc gtgctggtct ggtttgtcac ccggcgcgac
     1801 accaaaatgg cggtgctcac cgcgctcggc ctgctgctga tctggaacct cggactctgg
     1861 agcctcgcca tgctcacnnn nnnnnnnnnn nnnccgctct ccggccggcc ctcgacctca
     1921 tgcagaccat gccttcgttc gtctacctca tcccggcggt gatcttcttc gggctcggga
     1981 gcgttccggg catcatcgcg acggtcgtct ttgcgatgcc gccggcgctc cgcctcacga
     2041 atctcggcat ccggcaggtg ccccgtgagt tgatcgaggt cgcggacgct ttcggcacca
     2101 cgccccgcca gaaactcttc aaagtgcagc ttccagtcgc actccccacc atcatggcag
     2161 gggtgaacca gtgcatcatg ctcgccctct ccatgaccgt catcgcatcg atgatcgggg
     2221 cgggagggct cggctaccag gtgcttgtgg ggatccagcg ggtgaatatc ggcatgggat
     2281 tcgaggcggg gcttgccatc gtgatcctcg cgatcatcct cgaccggatc acccagaacc
     2341 ttgtgccgca acatggtgag agccggtgac ctgtcccaaa aatatctttg agaagaacaa
     2401 gcggtctgct catcctcccg catcttctct cgtgctcttc aaacacataa cggataaccg
     2461 gcgcatcggg aatggttttc cggccgcccc cctcttaaaa tccggtttaa gggaacttcc
     2521 gtctgtaaaa cggaacccgt gaactacccc gccctgaagg acggggcttc ctgcttcatc
     2581 cttcctctac tgaggcaagt ccacaggctc tatggcgcgt tccgcacctg ctaccccgac
     2641 aaggttctg
//
LOCUS       DAJB01000140           10005 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_57971, whole genome shotgun sequence.
ACCESSION   DAJB01000140 DAJB01000000
VERSION     DAJB01000140.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_57971"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1836 a         3237 c         3017 g         1911 t
ORIGIN      
        1 gttttaaccg ggggtagttg acagaacagt atcccgaagt tccatggcgt ttcatcgctg
       61 gaatgcggga caaactcatc caccactatt tcggtgtcag tcttgagatc gtctgggaaa
      121 ctgcacattc cgacatcccg acacttaagg aacggctgaa ggcagtccac gtccctggca
      181 ggcaaaacgg gagataacca tggacgcggt gcaggtgacc accgaaaggt acccggaagg
      241 gcacgccccc tctcccctga cccgaccggc cagcccccct cacgcctcct tgagccgctc
      301 gacgatcgtc ttctgcaggt agttgatgag cggcatatcc gagccgatca ccgcataccg
      361 tccggacccc gcagtggtaa tggcgaagag atcccggccg tcgaccagga tcgcgcactc
      421 gtccatcctc acaccgtctg gcccggactc atccattgcc ggccgggcat gcaggagttc
      481 gatcacggtt ccttcggcct cgtagatggg aagattgatg tcggcgtact cctctctcct
      541 ctgcacgacc acgtagagat cgatcttcct ctcaagcggc ttgatgagcg tatagtatct
      601 ccggaggaac tcggggttgt agcagaggat caccatgctc ttcttcaccc ggcggaagag
      661 cgaggagata tggttctcaa tcgcccagcc gctccggagg atgaagaacg agacaggagg
      721 aaggcgcggt ttgaccgaga ggctctttag cgcctcgcgt gtctcatcaa gggagcggat
      781 gtaatcgtct tttagccggt caaagaccac gtcgacgtcc agcacgtagt aggaggctgg
      841 agaaccctct tccaccccga tgaacccccg ccgggccagg tcgtttaaga tctcgtagat
      901 acgtccccgg gggaccccac ttatctcgtg gatgtcccgc gcggtcgctt tctgcagccc
      961 gacaagcgtg gagtagacgt tcgcctcata ctcgttcatc ccgagagccc gcagcccggg
     1021 gagaatctca gcagtcattg cacctctatt gagttgcaac acacttatgg tttccgggag
     1081 agacggatag atcggcgaga gagaacccat ctctcgcaag aacgtccccc ataacagccg
     1141 cccgcagagg ccaggcatac accccgaacc gacccccacg cagaacgggt cggctcgcca
     1201 ggccgggaat acccaccttc gaagaaggct gtcccggtga ggacgaatta gagcagcacg
     1261 caagagcgat accacctgaa ccaacagata ccccggatag gaatatcctc cgaagaccgg
     1321 gagatcggtc atcaacatgc cctggaacgt atccggcggc gggcccgggg tgtggctgac
     1381 ccgccgcccg aactgctgcc tctcccgggg atgcttctat tccacggtat gccgggacag
     1441 gcggcacctg ccgtcagtac cctgcaagga gcgtggaaat gagccgtgcc tctgcctttc
     1501 gcaggtgctc tatcgcggtg gctttgctga tgcccacctt ctccgcgagc tgctggctgt
     1561 tgatcctacg agggtactca tagtagccat actgttttgc cgcgatcagc aggtccctct
     1621 gccggggagt gagccgtgag aggggatcgt tcccggaaaa ggttgcttcc tcgaagatga
     1681 cgttccggac ctccccgtag gtggacatga gacggaggat cttgttcagg ttctcggcct
     1741 cgccgatgca ggcgtagacg aacaggtcgc gagacttgta tatgggggag ggccagatga
     1801 catccaggtc gaactcgcgc atcttctcca gcaggaactc gtcccggatg acataccgca
     1861 caagacaggt gcaagcccgg ccatcggcct tgagcacctc aagagacccg aggatttccg
     1921 ggaactcaag atcctcgatg gcgtatccct ctttcatcgt gatctcggca accaccgtct
     1981 cctctccccg ctcaaagttc atcttgagca gctcgaggac tttcacggat tcgaccctgt
     2041 ccaggaggtc tttcaccgcg cccggttgag agcctctgcg tcggggggca tgcccatcag
     2101 cggatccttc aggcagacct cgacggtcat cttgcgcata catcgcgtgg gaccctgccg
     2161 cagataaaac ccgtacatat acgggagaat cttgaacacc ccggttctcc agtactgatc
     2221 gatatgggaa gcacacgaga accggctgca aacgatcctg tgcgggcacc ggacgccgat
     2281 accggtcccc gcccgggttg cagtaagttc atctccggtc ccacggaaac agtacctcca
     2341 gcagatgagc aatatatagc gaggagagac gtgaactgca caactacccg tagcaccgga
     2401 ccctgccggg gttccgatga acacccgtgc cggaggccgg cgcatcgaca gatgtcatat
     2461 aaggagccgt ttcatgagga gaccgacccg gtatggaccg aggccgggtt cctccccccg
     2521 gcagactgtc agcgggccgt ggccctgctc caccgcctcg ccatggacga tgagcccgta
     2581 gcatccctgc tctcgtgtgt tctggccgca gcggcgaaat ccggcgagac aggccgcagt
     2641 taccggatcc tcgaccccac caggctccgg cgcatcgcgg aggagtacgg catcgagccc
     2701 accggaagga gcgacgacga ggtcgcccgt gcggtgactc tcgcgatcat cggcgagtat
     2761 gaggtgaaca tgccggagga gcacgcatga agaacccgta cgagtggctc gcaagcgcca
     2821 tcaacaacca cacctggacc gtcgccggag tcgctgcagc catcttcatc gtggcgtgct
     2881 tcggcctctc gatggtgtcg atggagaccg gcgacgacac ctacctcgat aagaccacgc
     2941 cccgcggcgc gctcctggat cactacaccg agacatacgg ctccgacgcc atcatgatca
     3001 tctatgaggc ggacagcgta cgaaacaccg agatcctcag gtacatcgac aacctccagg
     3061 aagatctcag gaatgaacgc tacgtcgatg aagtttcagg cgtcgtcgat ctccttaaac
     3121 aggccaacgg cggcaccctg ccgtcgtcca aagcggagat cgacgggatc atctctcagg
     3181 ccccgccgga gatggtcgag aggatgctgc cctcggacct gatgacgatc agcgtcatcg
     3241 ccctcgaacc gggcgtctcc caggatgcac agatgcaggt tctcgataac atggcaaccg
     3301 ccatccgcat atcgggaccg ccgccgggcg tcacggtcac cgtatccgga gaaccggcgt
     3361 tcgtgaagga gatgagcgag gcgatggggt cccagatggg tacgctgatc ctcgtagcca
     3421 tgcttttaat ggtcctcgcc gtgacgttcc tctttgctca cgtcagatac cgtctccttc
     3481 ccgtcgggat cgtggcagtc gggctcatca tgaccttcgg gttcatgggc ctcttcggga
     3541 tcccgatcag catgacggtg atcgcggcgt tcccggtgtt gatcgggatc gggatcgatt
     3601 acgcgatcca gttccacgcc cggttcgacg aagagatccg gcaaggcacg atcccggagg
     3661 ccgtacgggc caccgtcacg cagatgggcc cctcggtcct gatcgcgatg tcggcgacgg
     3721 cgctcgggtt catcgcgatg ttctttgcgc cggtgccgat gattgccgac ttcgggatgg
     3781 tctgcaccat cggggtaatt tgctgctacg ttgcggcgct catcatcgtc ccggtctttg
     3841 taatcgtcac ccgctaccgg gcgaagaccg gggccacacc ctccgacccc accccggaac
     3901 ccgggaactc cttcatcgac cgctatgacc gcctcctcgg gcggctggcc tacaccgtcg
     3961 caaaacatcc aatctcggtc atcctgctct ttgcagtggt cgccgcgggg ggcttctacc
     4021 tcgaccagag cgtccccatc agcgctgacg agaagacctt tgtccccgat gacatgccgg
     4081 ccctccggaa tatggagaaa ctcacccgga ccatggggtc aaccagcacc atcccggtcg
     4141 tcgtgctcgc agacgacgtc ctttccccgg atacgcttgc ctggatagac cggttcgggg
     4201 tctacgagca ggagcacaac gacaagatca ccggcgtcac cagcatcgca accctgatcc
     4261 gtgagaacaa cgatggggtg ctgccttcaa ccgagagaga ggtcgacaat gtcatcgccc
     4321 ggatcccgga ttcaaccctg aagagttacc tcgatgggaa catggaggca gtggtccagt
     4381 tctcgacggt cgatatggag atgagcgtcg cccgcgacct cgtcgagagg atacagggcg
     4441 acgttgtgtg gaacaacccg ccggccggcg tgacggtgaa gatcaccggc accatggaga
     4501 tgttttccgc cgtcatggac gacatcagcg agtcaaggct gtacatgacc gcgctcgggt
     4561 ttgccttcat cctcgggttc ctgatcctgg tctaccggaa attcggcgct atttcgccgg
     4621 tcatcccgat cgtcttcatc gtgggctgga acggggcgat catgtacctc ctcggtctcg
     4681 actacacgcc gctgacggcc gtcctcgggt cgatgacgat cggggtcgcg tccgagtaca
     4741 ccatcctgat catggagcgg tgcgaggagg aacttgcccg gggcatggag ttcctcgacg
     4801 ccatccagac cgccgtgcag aagatcggga cggccatcac ggtgtccggg ctgacgacgg
     4861 tcttcgggtt ctcggcgctc accctctcga acttcaacat catcagcaac ttcgggatcg
     4921 tgacggtcat atcggtcggg ttctcgctcg tgggcgcgat catcgtgatg ccagcaatcc
     4981 tctcgctgat gcaccgctac accggacact cccgggtccc ggcggcggcc ggggtcccgg
     5041 agtaacctct ccgccatcct tcgaagacct tcggtcttct catgctcctg ctctgcgacc
     5101 agtcgcactc cccggcaacg gagaccctgc cgccccccgg gtcgcagggg catcaagatt
     5161 tatatagtac ctgccatatc cttcccccgg tgatataccg tggcaaatgg agacctcacg
     5221 gcgcaggttt tggcgccatt tgagccggtc gaagaactgg cccgtaacgt cattctgttt
     5281 ctacccaacc tcatcgcagc gatcatcatc ctgatcatcg gctggatcat cggacgcatc
     5341 ctcggcaggg tcgttgccga actcctcgac cgactcggtg tcgacgacgc actccgaaag
     5401 acatcggccg gcaagcagat tgaacgatcc acgatgaaca taagccgcct cttcgacctc
     5461 atcgtccgct ggttcatcta cctcatcgcg atcctcgcag cggtcagcat cctccagatc
     5521 ccgaccctga cagcggcggt cgcggctatc gtggcctata tcccgaacat cgtcgcattc
     5581 atcatcatcc tggtcgctgg attcatcctg atcgactggc tgatcgacat cctccagaac
     5641 atgggggaga cgcggcagat ccagggcttc ggattgatcg cgatgctgct gcgggcgttt
     5701 ctctacttcg tcgtcgccat cctcgcgctc cagcagctct tgatcgacct ctcgatcatc
     5761 tacgtcttcg tcgtcccggt cgcctgggga gtcgggctcg gcctcggtgc ggcgattgcc
     5821 atcatcgtcg ggctcggcat gcgcgaccgg gcaccggaac tcatggacca gttgataggc
     5881 aaagtgaaag gagaataatc ctttttttcg ttggcgggct tacagcccgc cgtacttctg
     5941 cacgtgctct ctggcacgcc ggtagcgtgg atcccggtca ccggcgggag cgtcccggcc
     6001 gtagtgccgc cggtcctccc ccaccgggca gactttgatg cagatcccgc agggggagat
     6061 gtggcgccgg gcgagttcag cgctgttttc ggcgcaggcc gccttatcgg tgaggccctc
     6121 agggtagtcc cgctcgtcga gcgcgctcac ggggcaggag cggacgcaga gcatgcaccg
     6181 ggtgcagaga tccttctcca gcagggggtc ttgcggcagg tctgcggcgg tgaggaccga
     6241 gccgaaccgg acacgggggc cgtactccgg cgtgaggagg gtgttgttca cgccgaaggt
     6301 cccgagcccc gcgaggaggg cggcgtgacg gtgcgagaag aaggcgacgg ggttcttttt
     6361 gagcacctcg atgctcccgt agccgtcccg ggggacgaag accgaggggt agccctcgtc
     6421 gttgaggaag gaggcgagcc ggtaggtgta ctggtcgagc agggtgttga cagtcttata
     6481 caactcgtga tagtagatcg agggcgaggt ctcgagcacc gggaggtgga ccgggaggcc
     6541 gatgacgatc accgaccggg cctccggaaa gatcgactgc gggtagaact cctccggcat
     6601 ccaaggcaaa aacggcgggt tctcccaccg gcctgtgctc gcgatcccga cgagcgggat
     6661 ctccatcctc cggcaccgat cgaggacggc tgccttcagg tcgctgttca tgggtatcnn
     6721 ggggccggcg gtcttcccgt aatcccccgg cacccaccgt tcaccgtccc ggtagaactc
     6781 cttcttccag atcggcacgg tctgcttgag ccggtcgatg atatgctcgc aggcagcgaa
     6841 cgcctctttc cggtgcccgg ctccgacgat gacgagnntc gatcgggaac tcccgcatcg
     6901 cctcgtcgcg gatcgtctcc agttcacgga ccgcgacgtc ctcgtaggct tccagctcta
     6961 tccgctccac cccgtcgtcg tcccgaacaa cgccgcagaa ggtggccagg gctcccatac
     7021 ccggcctctt cgctgcctcg atcatcacac ctgcgtcgat gacgtcccgg gtaatatcaa
     7081 tcatatgtct gtatcccccc tcatccgccc gcaaccggcg ggaagatggc gacctcgtcg
     7141 ccttcggcga gggtatcgag gttgtttctg ttgacccgtc taccgttccg catcaggatg
     7201 acgtgcgccc ggagcgcccc ggtctcgtcg aagattgcat cgccttcgcc tccggcacga
     7261 tcccggagcg cggcgagaac cgctgccatc gtcgctccgt cggggacctc gatcgagagg
     7321 tcgctcccga ggatctcccg gaagcgggcg aatgccttca cccggacctt catggtcgca
     7381 cctccccgcc gcaggcaggg caccccggcc gccgctctac ggcaaacgtc tcaagcgtcg
     7441 tcgcgagccc gtcccagagg agaagccggt tcaacaggag gtcgccggtc ccggtgatgt
     7501 acttgatcgc ttcgttcgcc tgaatgaggc cgataacccc cggggtcgtc ccgacgaccg
     7561 ggaagacctc gccgggcggg gcctccggga agatgcactc gaggcaggcc gtccggccgg
     7621 ggatgatggt cgtcgcctga ccgtcgaatc cccggatggc accgtggatg agcggcacgc
     7681 cgctccggag cgcctcccgg ttcaggaggt agcgggtcgg aaagttgtcc atcgcgtcca
     7741 cgatcaggtc ggcaccggcg accaggtccc ggacgttcgt ctcgtcgatg gtcgctgcaa
     7801 gggcctcgac ccggatatcc gggtttgcct cccggagttt cgcttccgca gatcgggcct
     7861 tcaactcgcc gaggtcgctc tcccggtgga ggacctgcct gttcaggttg gtccggtcca
     7921 cggtatcccg gtccacgaga cggatctccc cgatacccgc gaccgcaagg tagagagcga
     7981 tcgggcagcc gaggcctcct gccccggcga tgaagacctt cgccttcttc agccgctcct
     8041 gcccctcctc gccgaagagg aggatctgcc gggaataacg ctcccgttca cgctcaagga
     8101 gcatatcgtt attctccact cttcgcaggc atcccgtgcc gtgtccggta gtcgtcgatc
     8161 gccttgtgga tcccctcttc ggcgaggacc gagcagtgga ccttctgggg gggcagcccc
     8221 tccagcgcct cggcaaccgc ccggttggtg accgcccacg cctcgtcaag ggtctttccc
     8281 ctgatgagct cggtggccat cgagctcgac gcgatggcgg cggcacaccc gaaggtcttg
     8341 aactttgcgt ccacgatcct gttctcttcg atccggaggg ttatccgcat gatgtcgccg
     8401 caggccgggt tcccgacctc gccgataccg ttcgcatccg ggatctcccc catgttccgg
     8461 gggttcgtga aatggtccat gactcgttcg ctgtacatag tgatcactcg tgtcatccgg
     8521 gggtgagcgg cgacatcttc cgcagccgct cgatcacccg ggggaggacg gaaagaacgt
     8581 aatcaacgtc gtcatcagtg ttccggtggc cgagggtgag ccggagcgag ccgtgcgcct
     8641 cctcgtgagg gaggccgcag gcggtcagga cgtgcgaggg ctcaagcgac gccgaggtgc
     8701 aggcactccc ggtggaggcg gcgatgccga gcgcgtcgag catgagcagg atcgactccc
     8761 cctcgacgta gcggaacgcg acgttgacgt tgtttgcgag ccgctcgacc gggtggccgt
     8821 tcaaccgggt gtcggggatc gtctccagga tcccccggat cagccggtcc cgcatctcga
     8881 ctatccggcg gttgcgtccc tcgatatcgg cggtcgcaag ttcgatcgcc ttcccgagcc
     8941 caacgatacc ggggacgttc tcggtcccgg cccggcggcc ccgctcctgc gccccgccgt
     9001 ccatgaacgt cgttatgcgg gttccccgcc ggatatagag cgctcccgtc cccttcgggc
     9061 cgtagaactt gtgggccgag agcgagagga gatcgatccc cattgcatcc acggcgatcg
     9121 gcaccgcccc gatcgcctgg acggcgtcgg tatggaacgg gacaccgtgg tcgtgcgcga
     9181 cttttgcgat cgccgccacg ggctggatcg tcccgatctc gttgttagcg gccatcaccg
     9241 agacgaggat ggttttgtcg gtgatcgcct cctcgaccgc cgccgggtcg accaggccga
     9301 actcgtccac ggggaggtag gtgacccggt agccctgctt ctcgagggcc tggcaggggt
     9361 ggaggaccgc gtggtgctcg atcgaggagg tgacgatgtg gtcgccgcgc ttccggttcg
     9421 ccgctgccac ccctttgatc gcccagttat cggcctcggt ccccccggag gtgaagaaga
     9481 tattctccgg ccgggccccg atcgcctccg ccactcgccc tcgcgccctc tccatcgtca
     9541 cctgcgcctc acgggcgagg gcatagatcg aggaggggtt cccgaaccgc tccgtgaagc
     9601 aggggagcat cgcttcgacg acctccgacc gcgtcggcgt cgtcgccgca tggtccatgt
     9661 agaccgaccg tgatctcccg tcagccatga tgctcacacc gtatcccggc cctgcgcaaa
     9721 ggtatttgtt ctccgaagtc aaagttaaca attgttaaca tcaatgttag attcgctcag
     9781 gaaccattaa atgttgtggt atgatgaaac tcccctgcca gttgattgta tggaatgtgt
     9841 tgcccgcaat ccgtgctgcg atcgcggaag aactgaataa aataggcgtc tcccagctgg
     9901 aggccgcccg ccttctcgat atgacgccgt cggcgatctc ccagtaccgc accgggaagc
     9961 gggggtaccg gatcgtcttt gagggcgagg tgaaagcgtc gctca
//
LOCUS       DAJB01000141            5448 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_96383, whole genome shotgun sequence.
ACCESSION   DAJB01000141 DAJB01000000
VERSION     DAJB01000141.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_96383"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          958 a         1789 c         1714 g          987 t
ORIGIN      
        1 tttaaccggg ggtagttgac tcccctatga aaccgttcat gaactgctcg gagacgaatt
       61 cgacctggag aagatccggg tgtttgcggg gaaggatcgg ttcaacatga ccgcgctcgc
      121 gctcagtttg agtacctacg tgaacggcgt agcccgcgcc catatggagt cttccacgag
      181 acttttcccc ggctaccata tccgggcaat tttgaatggg cgtgtacacc cttcaaagcg
      241 cattgaaaaa caagaggatc tgtatcatgc tctgggtgtg aagccgccag cctcgttatg
      301 agtgagcggg aatataggtg tcatagtagg gcgtatcctc gtaccagtat tcctttccat
      361 tgatgatctt gattcgaaca tctggcttca tggctgtcag tacaatataa gcgcacatta
      421 tatttataat tacctatact cttcgaaaat ataatggtca aaataggttc agaaagtaat
      481 tttgaagatg attgatgatt cgattgaaag caattatgcg cttattaaac gggagttgag
      541 gctggtgctc acgctcactg tgttcgcgac tcgaaaccct tcgggtttct cacgttcgct
      601 ttcgctcacg cctcgaagcc taatggcttc tcgaactcct gcccttcgag cagtcgttcc
      661 tcgaaacgct tcgagcaact catgtccccg ccgttttgcc ggaccccgct agaagcctgg
      721 cccaagagac ccggcagggg cccctttccc ccctcacttc agcaaatccg caagctgccg
      781 catctcccgt atatggtact gcccttcggc cgtcggggtg ccggcatggc agaaggcgaa
      841 ctccgacccg aagaggggca ggactggacg ggcgtagcgg aactcggcgc ccatgatgct
      901 cgtgcagatg ggcttctggg cctggtgggt aaacatgacg agttcgaaga ggtcgtcctg
      961 ggtccggggt ttcacgacga tcttcgggat atcgccgtaa gacctgagca tctcctcgat
     1021 ctttccgagc tcggggaggg tgggcatctc gttcgtgtgc agcgatgcaa ggatctggac
     1081 cccctgactc ttgatgaacg gcgcgtggtc ccggtagcgt gcctccacgt ccacgatatc
     1141 gacgtatctc gcgatcggcc cgacaatccg ggcccagagg gccgcgtcgc cgacaaaccg
     1201 ccctccctcc tctctgctcc tgagcgtggc aatcaggggt atggcggtct tctcccggat
     1261 cgcacggacc tggtccagca ggtctccctc catccggtca agcctgacct cgatgaacgt
     1321 cgggttgtac tccgccacct catcgataac gccggcattc tccacagaga cgacgatctt
     1381 catacttacc gaggtataac acccttatca cttaaaagcc tgcccgctat ctcccttccc
     1441 caccgtatcg cagcgctcca gcatggcggc gaagacctca tcgaagactg ccggcagcgc
     1501 ttcccggagc ccctgcaccc ctacctccgt gataccgccg ccggtcgcga cctccccgat
     1561 aaggtctccc ggcgccttgc cggtctcccg gaggtaggcg gccgtagccg cgaccatctc
     1621 ggttgcgagc acgagcgccc ggccgggggg gagtccgctc gcacgggctg tcgcgcccgc
     1681 cagttcctcg atgatggcgg caagcagccc gggcccgcaa ctcgagagaa gcgttgccgc
     1741 cgggatatca ctctccccga cgagcaccac atttccgatc gacgagaaga gcccctccac
     1801 cctgagagca tcatgcatgc ccacctgccg cccgtggcag accagcgtcg tcccctgccc
     1861 gactgcagag gtgaccgtag ggatggccct ggtgaccgac ccgggaaact ctccctcgat
     1921 ctcctgaagg ctgatgcccg cggcgagcga gacgatatgc tcgtccccgt ccatcaccgg
     1981 aacaatctcg tggaggacct ctctcatctc ctgcggccga acagcgatga tgatcgtacg
     2041 gcaccgccgg acgagttctc cgttcgttgc ggcgatgccg atccggggcc atgctgcggc
     2101 gcatgcgtcc cggctcttct ggccgcgaga agcaaccacg atctcatcgg tgccgagcac
     2161 cccggatgcg agaaatccgt tcaccagcat gctgcccatg tgcccgtagc cgataatccc
     2221 cacgcagtcc atacccaagc ctcctctcca catcgccttc ttgcccaaaa aggatacccg
     2281 ctcctgccgg ggtggaatat ataggccggc gaggtgaacg gtacccaaca gatgccgcac
     2341 gatcattatc aggggtccgg atcaccggaa ctgcttgcgc cggccgggtc gccggaggcc
     2401 ctggccgcag cggtcgccgc cggcgccgac gccgtctacc ttgccgggag gcgtttcggg
     2461 gcccggcact acgcggcgaa cttctccaac gaggaactcc gttctgccgt cgactacgca
     2521 caccttcggg gagtcagggt ctatgtcacc gcaaacgtcc tcgtccggga cgccgaactc
     2581 cccgacgtgg cgcgttacct cctctggctc tacgagaccg gggtcgatgc cgccctggtg
     2641 caggacaccg gggtggcgtc gcttgcccgg gaggtggtcc ccgacctccc ccttcatgcc
     2701 tccacccaga tgacggttca caaccgcgaa ggtgtagccc gggccgcaag ggaggggttc
     2761 tcccgcgtgg tgctggcgcg ggaactcacc ctccccgaga tcgaggggat cgcggaggac
     2821 gcggaggcac ggcgtgtcgg catcgaggtc tttgcccacg gtgcgctctg ctactgctac
     2881 tcgggccagt gcctcctctc ctcggtcatc ggggggcgga gcgggaaccg ggggatgtgc
     2941 gcccagccgt gcaggaaacc ctaccggctc gtggtcgccg agaaggacga ttacgggcgc
     3001 ccgaaggatc tccgggccgt gcctgcagag gaccgttacc tcctctcgac gcgcgatctc
     3061 tgtgcatacc cgcacctcga cctcctcgcg cgtgcaccgc tggcgtcgct caagatcgag
     3121 gggaggatgc gctcggcgga gtacgtcgcc acggtgacga gcatctaccg gcgtgctctc
     3181 gacgcgatcg ccgccgggga ggcctggtcg ccctcacgcg aggatatgca cgacctcgcc
     3241 ctcgcattca accgtgagtt cacggagggc tacatcctcg gcgctcccgg tatcatggcc
     3301 cgcgaccggc ccgggaaccg gggcatcctg ctcggcacgg tcattggcta cgacccgcgg
     3361 agagaggagg cgaccgtgcg tcttgccggc gacgtcgtcc cccgttccgg cgacgggctg
     3421 gccttctgca cggacgaccc cgaacaggac tcaggcgcgg tcgtccgcgg cacccccgcc
     3481 gtccgccacg gcacggtgcg actcagcgtc cccgcgcccg tgggagaggg agcccgggtc
     3541 ttcctgacga agagcgcgga ccttgaggag cgggcaaagc gggtcatgga agagccccac
     3601 caaccgttgc cgctcgacgt gacggccacc tgggaggacg gcacgccctg tctggaggcg
     3661 gtcctcgcac gacacggcgg cgaaccgctc cgggtacgct accgctcgga cctccgtatg
     3721 gagccggccc ggagccggcc gcttaccccg gaccagatcg cggagcagct cggaaagacc
     3781 gggggaacgc cgtttgcggt caacaagatg gacctccggt acccgggcgg gctctttgcg
     3841 ccgctcggag agttgaaccg ggtcaggcgt tcattcttag agaaggtcga ggaggccgtc
     3901 ctcgctgcat ggcgcccggg gccggatgca gtaagtgcag cccgggaaca agccgaagca
     3961 gcgattgccg ggatgacggg gccggctaag tcggagaggg agaagcggct cccgtcggtc
     4021 tcggtctaca ccgacaccct cgagggcgtg gaggcagctg tccggggcgg tgcccgaacc
     4081 gtctacctgg agccctctac tcccggtctc ctcgaagagg ccgctgccgt ctgccgggaa
     4141 gggggggcgg acctcgtctg gaaatggccg tcgatcaccc ggcgcgggtt cctcgatgca
     4201 gcggcgccgc tcctcccatc actttacgag gcgggcgtcc acggggcgat ggtgagcggg
     4261 cctggcgcag cggacgcggt gcggcgggcc gaaccacgga tgaacctcta tggtgccgcc
     4321 gggctgaacg tctggaacca ccgcactgcc agggaactgg ctccactctt cctgcggtgc
     4381 acggcctccc ccgagctccc ggccgccgat cttgcaaggc tcgcggagtc tgccgggggc
     4441 gcccccctgc tcgaggtgct cgtgcagggc aacgtcgagg cgatggtgac cgaggaccgg
     4501 ctgatcccgg cggtctccgg ggaaaggtct gacggccggt tcctgggcct cgaggaccag
     4561 cggaaccgcg tcttcccgct ccggtgcgac ggcgaggggc ggacctgcat cgcaaacgcc
     4621 gtcgaaacct ctctgatcga ccacctgccc gggatcgtcg cgatggggat cgacgccgtc
     4681 gccatcgatg cacgggggcg gggaccccgc tacgcagggg atatggccgg gatctaccgg
     4741 gcggggcttg acgctgcagg acggggagac cccggcatgc tttccgcgct caaggacgag
     4801 gtgaaacggc gggcgctcgg cggcatcacc ggcgggcatt tcgtccgggg acttccggag
     4861 tgagcttccg ccgggccccc tcctgaattt tcgtatcctg ccgatcacga gacagggcag
     4921 ggttcaggaa taccagtgta cttttgatga cggaacgtcg ttccccagac ctggtagtga
     4981 aggcgttcca aaacgcctct gaggtcgcgt cgaccacagg cccgatcaat ggaaatcgtg
     5041 gcgtcggatg cagaagtagt gcattccttt cagagcggtt cttctcaggc ttgtccggta
     5101 cactggagcg tggagatatc gccacgcggg ggcggggctg acggggaggg gggtatgccc
     5161 ccctcccctg tctccatccc ataaaaaggc gtgtaacccc cactccaccc ggcctccggc
     5221 ctcctccccc gccccccggg gcgggggcag tgcgtggcga taggtgatgt tcctacggga
     5281 catcgctgga gaggatcgca gatctttgga cttgcgacgt tcctctggaa tgaggctctt
     5341 gaaccgcaga tacaacttgc gactggctca attacttcag gcttgtcatg tcgcttatgt
     5401 tcacatacga cgggcaagga ggcccccggt ttcaaccggg ggaggaat
//
LOCUS       DAJB01000142            4335 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_28000, whole genome shotgun sequence.
ACCESSION   DAJB01000142 DAJB01000000
VERSION     DAJB01000142.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_28000"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          782 a         1353 c         1499 g          701 t
ORIGIN      
        1 gttctcgttg ttcgccccga cattctccat ctttcggtac ccccgctcct tggcctgccc
       61 gaggaggagc cactgcagga actcgttgcc gggcattgcg gtcatcttcg gggcccccat
      121 ccagagggtg aaggccttgt actcctgggt ggcctgcacg cccgtcaccg cgccgtcgcc
      181 gtcgtagagg tagtagagcc cgagctcatc gggatatgcc cggaagaggt cctcgaagta
      241 ggaccggctg atcatcggaa tgttcatatc cgggtcgctg aaccgctccg cgaccgcagc
      301 gtggaagacc gacagatcgg tgctcttctc gaaacggtag ccgctcttct cgactttccg
      361 caatttggtg cgaagggtag atgagaaacc gtcccagatc gcggagagcg gcgggtcgag
      421 gtcgatgacg tacgagaagt ggatccgggc atcgtagcct tcccggagga actgccggac
      481 atcgatgaac tccgggacct gggttatcgc gaggtagttc ggggccagag ccgcgaattc
      541 atcctcgagg tgtcctgcaa ctgccttgag catcgactcc ttcttgctct gtttggcggt
      601 atcatagcgg cggcccatga tgaacccgag gtacggtatc accgactgga cgggaggagg
      661 ggagaggagg acgttgatcc cgtgcacctg cttctggaag agcgggaaga tgcagatcaa
      721 ctcgttgccc ttgaagaccc cgtagggaag gaaccgaaag ccggtatgct tctctgtaat
      781 cttcataaag tcccatttat ggaaaaggcg gccggaggcg ctctcgtcca cgaacgtatc
      841 ccagctctcc ctatcgtcga taagtcccgt tgttatgctc tcactccctg ttccggggtc
      901 actgactgtc ccggcggagt atccatcacc tgggatatat atatttcgcc gaatcgaacc
      961 ggtctcggcc gggtgcccga ctccccgacc cggctgcggg cagtgacgac tctatggaat
     1021 cacaacgttc aaatcgcccg ggacaccaca taactgcgta ttcacctgca cggaggactg
     1081 agagggagat gctcacggat cgggaaccgg gacgctacag gcggcaggtc gcgctcttcg
     1141 gagaagaggc gcaggaacgg ctcgcaagtg cccgggtcgt catcgtcggg gccggcgggc
     1201 tcgggtgccc ggttgctctc taccttgccg ccgccggtgt cggggaggtc aggctggtcg
     1261 acggcgacgt cgtggacagg accaacctga accgccaggt gctccatgga gatcgtgaca
     1321 tcgggcgggc gaaggtcgag tcggcggccg agaaactccg ggcgcagaac ccggatatca
     1381 cggtcgttgc gacgtatgcc gccgtcgacg aagaaaacgc cgccgcgctc gtgggcgggg
     1441 cggacctcat cgtcgatgcc accgataatt accgggtgcg ctacaccctc aaccgggtgg
     1501 cgctcgacgc ccgcgtgccg ctcatccatg gggcggtcag gggctttgac gggcaggcga
     1561 cgacgatcat ccccggccgg acggcctgcc tggagtgcat catcccaatc gccccgcccg
     1621 aggagggcgc cacccccgtg gtcggcacca cgcccgggat catcgggctt gtccaggcaa
     1681 acgaggcgat caagtatatc accggcaccg gcgaccttct cacgaacagg ctgctcatct
     1741 gggacgggcg ggccgccacc atgacgaccc tgccggtcga gcggcaggaa gagtgcaccg
     1801 cgtgcggaag cggccggtgc tcgaggagat gaagaaatgc aggttcgggt cagggctttt
     1861 gcccggttgc gggaggccat cgggggggat ctcacactgg aggtccccgg gggggcgacg
     1921 atgagccggc tgcttgcggc ggtcggggaa acctcggagc aggcaggaga ggcgctcttt
     1981 gaggagagcg gagaactccg ggggtacgtg atcctgatgc acaacggcag gcgcgtgcgg
     2041 cgtgcggagg ccgggacgct caccctcgcc gacggggacg aggtcgcact cttcccgccg
     2101 gtggcgggcg ggtgacggag ggactggaat gatcagcgta acacgagacg atttcgacgt
     2161 gaatgcagtg atcggccggg taaagcggcc cgatatgggt gccctggtca ccttcctcgg
     2221 cgttgttcgg gacgacggga tcgaggtgat ggatcttgaa gcttacgagg aggtcgcggt
     2281 gaaggagatg gagacgatcc gggacgaggc gcttgcagat ttcccgatcg aggccgtcga
     2341 tatcatccac cgggtaggaa ggctccgggt cggggagaac atcctcctca tcgtcgtcgg
     2401 cgccggccac cggaaggagg cgttcgacgc ctgcgagtat atcctcgagc ggatcaagca
     2461 gagcgtgccg atctggaaga aggagatcgg gaaagacggc gagcgctggg tgccgggcga
     2521 gtcccacggg ggcgacgcat gatccggctg cagtcgtgcc ggaaggccta cgcgaaggag
     2581 acgcagcgga cggtcccgcc cgaagagacg cttcgccggg cgcgggcaag actcccggcg
     2641 gcggggatca cccgggttgc cgacatcacg aacctcgacc ggatcgggat cccggtcttc
     2701 tcgagcatcc ggccgaccgc tgaagcggga gcgatatcgg tctacaacgg caaaggcgcc
     2761 accccgatcg aggcggaggt ctcggcgatg atggaaggga tcgagcgcta ctcgggagag
     2821 atggacgacc ggcagcccat catcgggagg tacagcgagg tctcggcccg gacgaccgcc
     2881 ctcgacccgg caaccctgat cctgcccgaa cgggtccccg ccgatatagc ggtcccctgg
     2941 gtcgggggat acgacatcgt gcaggaggag gaggtgctcg tcccggccca cgcggtcttc
     3001 caccccctcc cgctgacctg cggccgcctc ttcaggacca acaccaacgg gcttgcctcc
     3061 gggaacacgc ttgaggaagc gaccttccat gccctgatgg aggtggtcga gcgtgacgcc
     3121 tggtcgctcg tcgaggtcac ccggaacacc ggcccccgga tcgaggggat cgccggcggg
     3181 ctcgctgccg acctgctcgc gaagttcgcg gccgccggcg tcgaggtcac cctcaaggat
     3241 atcacgagcg atatcgggat ccccacggtg gccgcggtgg ccgacgacgt ggtgcttaag
     3301 gaccccacac tcctcacgat cggcatgggg tcgcacacga gcgcccacat cgcggtcctc
     3361 cgggcgctca cggaggtcgc ccagagccgg ctgacccaga tccacggcgc ccgggaggat
     3421 accgatacgg cggacgtcag gaagaagatc ggctacgacc ggacgaaacg gctgaaccgg
     3481 cactggttcg cggagtccga aacggtcgag tttgcggcca tgccgtcgtt tgacagcgac
     3541 gacttcctca ccgacatcaa ccacgtcacc gaccggctca gcgtggcggg gctctcccgg
     3601 gtgatcgtgg tcgacctcac ccggccggag atcggcatcc cggtcgtccg ggtgatcgtg
     3661 ccggggctgg agatgtacgc gatggaccag gaccggatgg gaaggaggtg ccgtgacgca
     3721 cgaagtcgtc gtcttcctcg gtcccagctg tgatcacgcg accgcccgga agatcctcga
     3781 cgccgattac cgtcctccgg caaaacgggg cgacataacc cgggccgcag acgccggggc
     3841 ccggatcatc gggctcatcg acggcgtctt cttccaggac tgcgccgtag cgcaccggga
     3901 gatcctcgcc gcgctccggg ccggggtgcg ggtcgtcggc gcctccagca tgggcgcgct
     3961 ccgggccgcg gaactcgaca ccttcgggat ggagggcgtc ggggagatct accgtgccta
     4021 ccgggaggga aggctcgtcg ccgacgacga ggtggcgctc ctcttcgacc ctgagacgtt
     4081 catcccgctc tcggaaccgc tcgtgaacat ccgggcgacc ctgcagcggg cgctggaatg
     4141 cggggtgatc ggcgccgacg ctgcccgggt gcttctcgac gcggcccggg aactctattt
     4201 tcccgaacgg acctacgacg aggtcgtcga ggcggcggcc ggggcggcgg atccggaaga
     4261 cctcgcccgg ttcgcggcat ttgccgcgga gcacgctgtc gaccaaaaac gcgaggatgc
     4321 gctgctggca ctcga
//
LOCUS       DAJB01000143            3504 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_26675, whole genome shotgun sequence.
ACCESSION   DAJB01000143 DAJB01000000
VERSION     DAJB01000143.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_26675"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          581 a         1104 c         1137 g          682 t
ORIGIN      
        1 ccgctcatgc actcgtgata gatctcgacc tggtcgccgt cgatggtggt gaagacccgg
       61 cggcaggcgc tctccggcat cacgctgctc gccgcgtagc gggccgcgtg ccgtccccgc
      121 tgctcctcca tgagttggac acatccctcc tcgatgaggc ggttcaccca gtcctggacg
      181 gtgctccggg gggtatcggt ggcctccgca atatcggcca cggtaaagga gcccctctcg
      241 agcgtcgcct gacgcatcag ccggaggtac tgttttctcc gctcaagaac cctggacata
      301 ccggtcatgg atgtagagga gatcgccgat cacggcgctg gcggtctccg tggaacccgc
      361 gcccttcccg atgaacgtga gatccccggc aagatccgcc tccacggtga cggcgttgag
      421 tgttccttcg acaacgagcg ggtgcgcctt gccgacgatc cttggtgaaa cgcggagcac
      481 ccccgccttg gggacgatct cgccgatgag ccggatggtg cagtccaggt cttctgcgag
      541 tcgcagggcg tcgggtgtca ggagggtgat ccccgtcctc tccacgtcat cgagcgtggt
      601 atgcatatcg aggatggtgt tcgcgaggat gaccagttta acgcctgcgt cgatcccctc
      661 gacgtcgtag gtggggtccg cctcggcgta cccgagttcc cgggcctcgg caagggcctg
      721 ctcgtaggtg aggctgtcct cggccatccg ggtgaggatg tagttgcagg tgccgttgaa
      781 gaccccgtag agccgggaga tgctgttccc ggcgagcccc tcctgcatgg catggatgag
      841 cgggatcgcg ccgcagaccg tcgcctcgta cctcaggagg acgccatttg cctccgcgag
      901 agcccggagt tcggggtagg cgagggcgat cggacctttg ttcgaggtga cgacgtgctt
      961 gtgccgcccg agggcccccc ggatatggcc cagtgccggt tcggcggtct ccacgttcgt
     1021 cggggtcacc tcgacgagga cgtcgtaatc cgccttcgcc accacgtccg cggggctgac
     1081 gcccggggtg ccgcaggagt cgccgttccc cttacgggca agcgccgccg caacgtcgat
     1141 cccgccggca tcgatgatac cgctccgcga gtcggcaagg ccggtgacgg tgatattgag
     1201 gtcttttgca aggatagccc gggcgatgcc ctggccgacg gagccgaacc cgagtatggc
     1261 gatccgcatc atcatgcatc ctccaggggc tcgatgatga ggagtttctt ctgttccgca
     1321 acgcccctca ggatcctgat cgccttcttc atctccgtcc gggtggtcga acggatggtg
     1381 atgcgggcgg aagaaggggc gttgatcgcc ggcataacga gggagagttc cgtcacctct
     1441 gcagacccgg tccggtcgat ccggtcgacc gtgtcggaga ggtccgtatg catcaagtgg
     1501 ccgatcatga tcagcgtgca cttgtagagg agccgctcct ccccgatgcg cacgacgctg
     1561 acgccctgct cccggagcag ttcgaggaga gtctcaaggc gcccctccgg cagttccagc
     1621 acgatctgga catccagcgt ttccgcggcc gccgaggttt ccctctggtg aatgaccgcg
     1681 atgatgtttc ccccgacggc cgagatcggt tggagggctg cgaccagctg tccgggccgg
     1741 tctttcatct cgagtttcat ggatacctgc aaaaaaaccc acctgctgaa tggttgacct
     1801 gccggagaga taagcctttt accccggaac ggacccatcg acaggggagc gcgccgtaaa
     1861 aagagagggg gacagcggct ccgttaccgc accgacccgg gcgcaccggt gacgaccgtg
     1921 agggcccggg caaactccgg gaggatctcg gcggggatgc ccatgacgag ctcgtcatcc
     1981 tcgatgccgg agaaccggcg cgagccgtcg cacccgagcg agtagttgat cctgccggtg
     2041 agatagggct gcgcggtcgc gtccgcacag accgactgga tccccgacat cgtggactct
     2101 atccgtccgc cgagctggta gagcgcggcc tgtgccagtt tcagcattgc ccgcgggttt
     2161 gcaacgatga tgacgacatg cgggtcaaac ggcgtcctct cgagcggtgc gtagattgtc
     2221 gcataggtct cgagttcggg gacgtgcggc acctgccgga tggtccgcat gcaggcggcc
     2281 cagctctcga acttgcccag tttgtagtag aactctcccg tgcgaaggct ctgcgtgagc
     2341 tccttcagcc cgagggccca gccgccaccc atgcagacgt gtttgtcggc ggtcgcgtag
     2401 aagatctcgc cgtccagacg ggctttgctg accatctggc agtggcggac cgtctcctcg
     2461 atcggcccga ccccttcggg gatgccctca gggctctttg cgagtttgac cgcgaccggc
     2521 gacccccgca gatcgagggc aatcttgagc gtctctgaaa tctctgcgta agagatcttc
     2581 gtgcgcatct ggtcttccat aatcgtgctc actcctctgt ggagatagtc cccgtggtca
     2641 ctcataaaat ctcggttcgg ggtatcggca gggtcagagg ttgcgcagtt tgtggttcac
     2701 cttctcaaag aagggtttgc ccgtatggac gaagagggcg ggctgttgtg accttttaac
     2761 tgtgatcgta gcctccttct ccagttcaaa cgtgctctgc ccgtcgatga cgaggtgcgc
     2821 cggcttctcg gtctcaaggg tgatctcgag gttcctcccg gtgctgatga ggtgggggcg
     2881 cgacgagagc atatacggcg cgagcggtac gaggaggaac ccttcgatct gcgggtcgac
     2941 gatcggtcct cctgcgctca tggcgtaggc ggtcgacccg gtcggggtcg atatcagcag
     3001 gccgtcggcc ctgaatcgtt ctgcgggcgt cccgtcgacg tagacgccga accggagcat
     3061 ctttgccggc cggtcggtga caacgagccc ctcattgagg gcgtctccga gccgtttccc
     3121 gtcgacggag aggctgaccc gcatccggcg ctcgacccca aacccgcttt cgtgggcggc
     3181 gaagaatgcg tgggcttcgt caggttccag gtccgcgagg aatcccacct cgccccagtt
     3241 gatcccgagg accgggacct gcctcttcat ctggtggacg gtgaggagga ccgacccgtc
     3301 gccaccgacg accacgacca gatcaccggc gatctcctcg aacggtgtgc cttccgcacc
     3361 gagttgcctt gccgtgccct cttcgagcgc gacgtcgtgc cccagacctt cgaggtcccg
     3421 ggcgaggtct gcggtatagc ggagtgcatc cgcatcgtcg atgcgcgata ccagcacgat
     3481 cttcataaaa atcaggcagg agac
//
LOCUS       DAJB01000144           13240 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_83101, whole genome shotgun sequence.
ACCESSION   DAJB01000144 DAJB01000000
VERSION     DAJB01000144.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_83101"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    7538..7573
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2233 a         4366 c         4241 g         2364 t
ORIGIN      
        1 tgatgcaggc cctgctcgcc gccgacagca atgcacgctg gaccgtggcg atggcgctcg
       61 ccgagatcga gagagtgttt gagcaatcgg gcgaggcgtt cacgggcctt gccatggaag
      121 cctacgaggg gacgtgaaac ggcgtcctcc cgggacatac cttttttgcc gcagccgggg
      181 acggtgcgga tgaataacta ctaatagagc ccgttaacaa gagactagta tgcaagggcg
      241 tcatatcctg ctcctgatcc ttctcgcggc gaccatcatc gccggaggct gcacgacgcc
      301 ggaagaagcc ggggagacgc agccagcctc ccccaccctg tcgccggcgc cgactgaggt
      361 gcaggcggtg ggagcaggcc tcccggcccc cacccccgcc gcgcccgtcc ggggcgacaa
      421 gacaccgcag agcgtcgggt tcgtcgaccc gcagacctac catatcccga ccccgacgcc
      481 cacgatcgcg atgagaacgc agccagacga cctccgggtc tccgggaaga tggtgaagta
      541 cgcggtggtg acctgcgact accctcccgg cgttctcgct actgaggtct accacatccc
      601 cttcccatac tgggacctga acgtgagcgc aacgccgatg aacgagtatc cctggcttgt
      661 gatagaggtc cgggatccgg aggacccgaa tcgggtcgtg aaggagatcc ggtactcccg
      721 cggggatatg ctgtatgccg gaaacggcac cacacaaagc acctccgtag agaacagcgc
      781 aatcgaaaag agggagacgt tcaccatccg ggaagggtat ggagactact acttcaccat
      841 ccgctcgggg tccctgaagt cgctcgagct caccatcctg atcccggaga agtacctcgt
      901 ctaggcggga ggagcccctc ctccctcttc tgcaacgcgg agctcccggt cgtagtcccc
      961 tatcgcggtg acgtattcct tccattcctg atccgcccgg gcgaggttct cgtttgccgc
     1021 ggtgtatgcc cggatgtcac aggcggccgc agccgccgac cagagggaga acgcgcggcc
     1081 caggtggtcg agcgcccgga catacgccga ttgcgctccg acgtacgctt ccgggaccct
     1141 gtcggccccg acgacctgcc ggtggtaggc cgcaacggcc ctgccgtact ccgcggtggc
     1201 cgcaaactcc cgcacgtagg cgtcggcccc ggcggccgac gaggtgtcct gcaaatagcg
     1261 catataagag gagaccctcc ccgagaccag ggagaggtca gcctccgatg ccgccacgtg
     1321 actgccgaat accgcaacct gtcgatctcc ggtgtagctc gcgaagagcg tccacccggc
     1381 gaaggcgatc agcaccacgg caatccctac cccggcggcg atgagcgcct ttctccggtc
     1441 gatggtaact ttcgggagtt tgggaatctt caggtccatc gccctcacgc ccccgccggc
     1501 aggcgtaccg ggcgaatctg atactcaacg ggcgtgggga tgtagcggag ggggatgatc
     1561 cccggtgtcc ccgacctcac gctctggagt gacccgtcct gagtatcccc gatggggagg
     1621 ggctttcccc aatacgtcga gtttcccgtg gagatcgtag tatagttcgt cccctctacg
     1681 tagtagtagt gcttcccctc gtactcgaag tacttcggcg tgtacttcgc gtaccaggga
     1741 ttgaacccgt ccatccggat gccgagcgcc atgtgatccg ggtactggag cagcaccgta
     1801 tcatagccga gagcatccag gagccccccc atcaggattg ctgcatcctc gcagtcaccc
     1861 ttcccatcga caagcgtctc gacggggtac ttgggatact caacgccgcc ccccacatgc
     1921 cgggcataat gggggaggac cccttcggtg taggactctg cgctggtatc agattcgtac
     1981 gggatctgct ggacgaagaa gatgaggttc atcatccgga agtactcctc cgccggattg
     2041 gcggtcgggg gggcgatccg gtgggcgagg tcctcgagga tcggccggtc ggcgtcggcg
     2101 agcgcatatt ctccccaggc aagcgggtcg gcaaagcgcg gtgtctcccg gtgctcgata
     2161 aagagcgcct ccgggacccg gacctgcgtc gtctggacgc cgccgtcaat cgccgtccag
     2221 cggagggttc tggtgtaggc cgcgctgtcc tccgggaagg gcgtcgccgc cggcgccggt
     2281 gtgggtggga cgtatacggg tgacgtcgtg accggtgccg gtccggtgga gacgaggccg
     2341 atcggctccc cgaggagtgc gggtttcact acgagggcga cgaccaggat cacgagcccg
     2401 cccgcaagaa ccggcataat atccttccat ttcatctccg gggcctcacc gcgtatcggt
     2461 aataggatat gggcgacctc ccgctaaaaa ccttgactca tggtcgcggc cggtcccggg
     2521 agcccgcttc aaggatgatg cagacagaga gcgcgaggcc gaccccctgc cgctggagcg
     2581 ggggatcgac cgccagcggg gcgagcgcga gaaccatcct gccgccggtc gtcatcgcga
     2641 gggagaagag gatatggccg atgatccggc cgcccgctct ggttccggat cacctcgttc
     2701 cgggctcggg acctgtgggg caaacccctt caagcccggc cagctgcttc cccggcccgg
     2761 ccgcatacct gcagaccgag gggtagtacc tggtgatgta ctccttgagc atccggacgg
     2821 agcagtactg cggggcgatg ctcttgatcg actccttcat catccggacc catttgtgcg
     2881 gaacgttgtc catcgtccgc gagtagtaga gcggtgcgat ctcgttctcg atgaggtcat
     2941 agagtgtctc cgcgtccgcc tggttctttc ccgcataggg agcgtggcct tcgatccccc
     3001 agccgtttct gccgttgtaa ccctcgatcc accagccgtc gagaaccgag aggttcagca
     3061 ccccgttcat ccccgccttc atgccgcttg tgccgctcgc ctccatgagc gggacggggg
     3121 tgttcatcca gatgtccaca ccatggacca tgtagcgcgc aagctgctca tcgtagtcct
     3181 cgacaaacgc tatccgcccg ccgaaccggg gatcctggga atactggtag atctcctgga
     3241 ggatacgctt gccccgttca tcagccgggt gagccttccc cgagaagacg aactgcaccg
     3301 gtctccagcg gttgttcacg atccggtcaa gtcggtccag gtcctcaaag atgatatccg
     3361 cccgcttgta ctcggcgaac cggcgggcga accctatcgt cagcgccgag gtatcgagca
     3421 tgagcccctc ggccgcgagg ttcgcaggct cgtcacggag gttcgcccat ttgagacgtt
     3481 ttcgctcccg gatgcgggca aagagtttca tcttcagcca ctggtgctcg ccccagagtt
     3541 catcgtccgg gatctcgtcg accacctccc agacaaccgg gttatcgtag tcctcccgcc
     3601 agctcgggta gaccgggtcc atataccggt caatgagcgt ctcgatccgg tggtttaacc
     3661 acgtgggcat gtggacaccg ttcgtgatgg cgtcgatcgg cacctcctcg acggggaggt
     3721 cgggccagag gggccgccac atctcacggg cgacctcgcc gtgcttctgc gagacgccgt
     3781 tgtggaaacg gctcatccgg agggcaaagg cggtcatgtt gaacccggcg cccgggttgt
     3841 gcgggtcctc cccgagcgcc atgaactcgc cccaggaaag accgagcgac gggcagtaac
     3901 tgcggaagta cttcgccatc aggtcttcgg ggaagacgtc gtgggcggcc gggatggggg
     3961 tatgggtggt gaagaccgag gtgccccgga cctgctcgcg cgcctcttca ggcggcatcc
     4021 ccgctacaag ccgctcccgg aaccgttcga gaagggcaaa cgcagaatgg ccttcgttta
     4081 agtgcatcgc cgagtaatcc acgccgaggg tatggaggac cttcctcccg ccgagaccga
     4141 gcaccacctc ctgccggagc cggcactcga gttcatttaa gtaaaggcgg tgggagatgc
     4201 tccggttctc ggggaggttc tcgtcgatat gggtgtcgag gaggtagagg gggacccgtc
     4261 ctacctgcac cttccagacc gcgacgtaga tcggcgggtc gaccaggggg acctgcacca
     4321 caagctgcct cccggcgtcg cccgtcaccc ggagaaccgg tgctgcgtca cggtccagga
     4381 tctccgcgac gttcttctgc cacccgttcc cctcaatatg ctggtggagg tagccttcgg
     4441 cgtacatgaa cccaacggcg accatcggca cgccgagatc gctcgatgcc ttgatgtggt
     4501 cgcccgcgag gattccgagc ccccccgcgt agaacgggag cgagtgatgc agtccgaact
     4561 cgcttgagag gtaggcgatc gtcagcggcg accgcccggg atagtgctgt gcaaaccagc
     4621 tcgtccccgg caccatgtac tcacggaacc ggtgcatgat gatgtcgtag cggcggaggt
     4681 actccgggtt cgctgccgcc cgctcgaaga accgcggcgg agtctcccgg agcatcctga
     4741 ccgggttgtg gcggctcatc ttccactccg cccggttcag cagcttgaag agcaccttcg
     4801 ccgaggagtg ccagctccac cagaggttgt acgcgaggtc gacgagcccg aagatccgct
     4861 ccgggacgtg ggtgaactgg tcgtagggta tctccgtctt catctaaccg gcattacccg
     4921 tcgcacctga taagtgcttc tgtcacgggg acacccgggc aggagccggc tcaccgccgt
     4981 tcttcgcgca ccataacttc ccggatcttt ttctcctccc actccctgcc gaagatctgg
     5041 agcgcgtgga agaccacgcc cacctccccg aaggtagtca cccagtagaa ccagagcgtc
     5101 ccggggctgg acacaatgtt gatcgcgaag aggaggggtg acggcgatgt agaccgcaag
     5161 gtgcatgtag aacccccgga tctcctcgac ccgcctcttc gcacgggcat agctgtcctg
     5221 ttcgtccata tgttccggtt acgaaccctg atgtttatac ctgcctatgc cgcccggcgc
     5281 agacccggtg cggcaggcgc cggatggagg gcgggaatcg gatagcagcc aaccgcggat
     5341 acaccgtctc tcacggcttc tccgggggac ggcaccggca aggcctgcgc ggcgtgaggt
     5401 ggtctgacgg gaggggcggg actggaggga cggtttcgat gaattctgcc gtaagccggc
     5461 cggagagaat ggagaggtat tatatgcacc cgttgccgat gttccttcac cagaaatccg
     5521 gaggagcgtg actggagtgc agcagatgat cgaggcagac ggcctcacga aggattacgg
     5581 cagcgtcacc gcggtgaaca gggtatcgtt ctctgtcgca gagggggagc tcttcggcct
     5641 gctcgggccg aacggctcgg ggaagacgac gatgatccgg atgctgaccg ggcaggtcag
     5701 accgacgtcc gggtcagccc gggtgatggg gctcgatgcg gcgaaagacc ccatcggggt
     5761 ccggaagctc gtcgggatca tccccgagca ggagacaccg ccgagtttcc tcaccgcgga
     5821 ggagtacctc cacttcgtgg ccagcatccg gaacctcgac cgcgtcgacg agcggtgcga
     5881 tcgctggttc gatctcctgg agtttcagga taaacgggac gtcctctgca aggacctctc
     5941 gcggggcacc cggcagaaac tgatgttcgc acaggctttt cttcacgagc cgcgactcgc
     6001 cctcatcgac gaaccgctga tcaacctgga ccccatcatg cagcggacgg tcaaagactt
     6061 cctgcagagt tacgtccgtg acggcgggac cgtcttcttc tccacccaca tcctcgagat
     6121 cgccgaggag atctgcgaca ggatcgggat catccacaac ggcaggctcc ttcattccgg
     6181 ccccatcgaa gacctcaccg ggtccggccg gcacctggaa cccttcttcc tcgacctggt
     6241 acggggcgat accggtgcct gacctcttcc tcgcgatgat gaaagaggag tggcggatcc
     6301 actcgacaat cttcgggagc ctcgggttcg cgctcttccc ggcggtggtc gccttcttcg
     6361 ccttcctcgg atcgctcacc ctcccgctct tccgcgatgt gctcccgtac gccgcggtag
     6421 cgctcctcgc gcagatcctc tttctgctga tgggcgtgat ggtggggtcg ttcgggctta
     6481 tgggccgcga gttcatgaac cgccggttcg ggcaggcaag cctcgtcgcc tacgcctccc
     6541 ggagcctccc ggtctcggaa cggcggatat tcgccgcgtt cctggtgaag gatacggtct
     6601 actacatcct gctctatgtc ctccccttcg ccgtggggtt tgccgcagca acccccttca
     6661 tctccctcga cccggcctgg ggcctccttg cattcgtctc cctgacgctc gccttcctca
     6721 tgggcctctc tatcgtctgc ttcctctcga ccctctacgc acactctgtt gcgctcctcg
     6781 caggaacggt cgtggtgctc ggggtgctcg cgcttgcggc gctccatctc ggggcgatca
     6841 ccgtctccac cgccctcccc ccatacgcct tcttcctcga cccgggcccg ggaccgctcg
     6901 cgctcgcgct cctcctcatc ctggtcccgt cggccgtatc ggtcctcttc gtcaccgtcg
     6961 aatatccgga caggacaaaa cggttcaaca acagcctcga cccctttgcg gagaggctcc
     7021 gcgccctcgg gagtgcgcac ttcattgcca aggacgcgct ggacctgctg cggagcgagg
     7081 ggggtgccgg gaaggttatc ttctcgttcc tcctccccat cggcctcatc tgggtctgcc
     7141 tccaggtcct gatccggttc atcccgggga tcgatcccct cgtggtcttt gcggtcctcc
     7201 tcggggtgat atcggccacc atctacaact ggctgaccga gttcgactcc ttctcgtcct
     7261 acaccttcct gccggtggcg gtatcggaag tgatcgacag caaactgaag agctatggcc
     7321 tcctcggcct cctcccggtc gcagtgctcg tgctcgcggc ggcgacctcc ggcggggcgg
     7381 ggaccttcct cccggccctt gccgcgttcc tctcggtctc ggcctacacc ctcgcggtga
     7441 cggtctacct caccggcctt tacccgaacg taatgcttta ttccgccggg gtcttcctcc
     7501 gctacctgct ggcgataagc ccggcgctcc tgctcctnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnactgaca ctttcacccc ccgcacggcg cggcttttaa acccctgcgg
     7621 gagactccag aacagcaggt gcgccgcggc cgacgaggag gggagaccct gccggatcgt
     7681 cgagatctcc ctcgacggcc ggcggttcgg aaccagggtc ggcgaactcc ggcaggcgct
     7741 cgatggccgg cgacccgccc ggatcaggtt gctccacgat gaccggggcc cggttctcgg
     7801 ggatacggtc ggccgcgcgg agcggtcgcg gacggggaag gcggtcgtct tcgagctcgt
     7861 cccgggggag cgctactcgg tgccggcggc ggcgctccgg gccgtcctcg cccgggctgc
     7921 cgcttttgca cccgtctcgg caatcccccc ggggagcatc ccggccgccc ggcggcaggt
     7981 tcccgtcagc gggtaacccc ggcgtcgcgg aacgcccacc cgacattccc cggaccgagg
     8041 aggactccgc cgaagatcct tgagaccacc agcgcgtggc tcccgaggcc gcgcttgcgg
     8101 agcaggtcga gaagcaccct ccccggccgg ccgacctcac cgtcgttctt gaactcctcc
     8161 agtgttccgc accggagggc ggtgcagtgg tgggcggcct tccggtaggc ctcacggtgg
     8221 ccggcaagaa cccgggcgac gtcctccggc ctctccaccc ggtagaggtg agcgtagaac
     8281 cgcgatcgcc ggacctcggt cacggcggca ccgaggggct ctgcggtcac gcggccaccc
     8341 gcttcctcgt atactcatcc cagtgcgcct cgaggaaccc ccggataccc cggccgggtt
     8401 tatacggcgg gtatatccgg cggtaggccg tctcggcatc gtcaaggctc tccgtcggca
     8461 ggacgtcggt gtaggccgcg agatagagga gcccgatcgc cgccgatcgg gagatgccga
     8521 agccgcagtg gaccagcacc ggccggccct cgccaaggca ccgatcgatg aagcgcaggg
     8581 ctgcgtcgat ggcctccttc gggacgtcgt cggggttcct cgagtcgacc aggttgagca
     8641 tcaaccggtt cccccgccgg gcgaccaggt gttcggggtg gtccggcggc accgtgccga
     8701 ggggcgagta ggtgaccgcc aggcagtggt aggggctccg gcaggcgtgc accacgcacc
     8761 aggtctcgtg cgcctctacc gtaacctcgt agtcctcctg cgtcccgatg tacaggttcg
     8821 ggtagatctc gatcatgctc cccccgtcac cgcgcctcga gcgcgtacgc cgccgccccg
     8881 aggagcggtg cctgtccctc gaggccggag acgacgattc ggggaagggc aaggtagcgg
     8941 tcgatgtaag gctccatgtg ccggatcacg atatccccgt agtggagggc gagcggcccg
     9001 tcgagcacga tcacctcggg gttgtaggca acgatgacgc ctgagatccc ccgcgcgttc
     9061 acctccccga gcgcctccat gaaggcgagg gcgaccggat cctccatccg ggccgcctcg
     9121 aagatcgccc gggtggaacc ggcgtcgaag gttacccgcc gaacgtcgtt ctcctcccgc
     9181 cagaccgcga agaaccgggg gacgtacttc gccgaggcgt agccctccca gtggccggca
     9241 aagccgcacc cgcagacgag gttgtagcgg gtatcgacgg gggtgtgccc gatctccccg
     9301 gcattcccgc tcgctcccag gagcagacgg ccgttcacca ccgcaccgcc gccgatgccc
     9361 gtggagaggg tgacatagac gacgttatcg gagccgcagg cggcacccgc ccaccgctcg
     9421 ccgaggacgc cggcccgggc gtcgttgatc agggcgaccg gcagcccgaa ccgctcccgg
     9481 agcgggccgg tgatctccac gaccggaaag gcgatgttcg gggagttgac gacccacccg
     9541 cggccgagat ccagggggcc ggccgacgcg acgccgatgg ccgtggcctg ccttccttcc
     9601 ggcgccgcga ggagagcctc cacccgggcg gcgatcgcgg cggtgatcac ctccccggaa
     9661 gggcccgcgg tcggcgtcgg gacggcggca tgggccagta cggtcgcgtc ggagcctacc
     9721 agggcggcgc ggaggttggt tccgccgagg tcaacggcaa tcacggtcgt cataggggtc
     9781 gctcctttca ggtgcgggtg cttctcttct cggattacgt gaaccaccgg cataaaccta
     9841 cgccttcggc cggggcagcg gatccgggac aggtgccacg agcatggaag caaggtatat
     9901 ctacttcccg cacccagatt taggtatacc taaaaatgat cgccatggag aagacgttac
     9961 ttgatctgga accgggcatg cggggaaccc tcacccggtt tgcaggaaat ccgggctttg
    10021 tcaggaaact caggaccatc ggtatcaggg aaggcaagac cctcaccgtc gtcgccaggc
    10081 accctctcgg cggtccggtg gtgatcgaag tagacgggcg ggaagtgacg ctcggccggg
    10141 gaatggccag aaaagtcatc gtggacgtgg ccgcatgaag accgtcgtcc tgatgggcaa
    10201 cccgaacgtc gggaagagtg ggatcttctc gcggctgacc gggtgccggg tcatcacctc
    10261 gaactatccc ggaaccacag tcgacgtctc ccgcgggaag atgcgtcttc tagatacgcc
    10321 ggccgaagtg atcgacgctc ccggcaccta ctccctcacc cccgccaacc cggccgaaga
    10381 ggtcgccgcc gggatcttcg cgcaggcgga ccttgtcgtc aacgtcgtcg atgcgaccaa
    10441 cctggagagg aacctctacc tgaccctcga gaccctggag cgcggggtcc cggcggccgt
    10501 cgcactcaac ctctgggacg aggccggcca cagggggatc acgatcgacg tcgccagcct
    10561 cgcgcggctc ctcggggtgc cggtcgtccc gaccgtgggt ctcaccggcc aggggatacg
    10621 ggaactcgtg gagcggctcg gcgaggcgca cccctcggag acgatccggc ccatgagcga
    10681 agaggagcgg tgggtggcgg tcggccggat caccggatca gtgcagacga tcacccaccg
    10741 gcaccccacc ctccgggacc gggtcgcgga ggcaagcatc aggccgctga ccggcctgcc
    10801 ggtagcactc gcggtcatcg ccgccgcgtt tctggcggtc aggtggatcg gcgaagggct
    10861 catatcgctc gtcttcgagc cgctcttcga cctctacacc ccgatcgtcg gacaactcag
    10921 cgactacctc gggccggggc tcctgcacga gatcctgatc gggcacctca tcgagggcga
    10981 gatcgactac gtccagtcga tgggtctcct cacgacgggg ctgtatgtcc cgttcgccat
    11041 gatcctcccc tacatcgtgg cgttctatct cgtactctcg atccttgagg ataccggcta
    11101 cctccccagg ctcgcgacgc tgaccgatac cttctttcac cggctcggga tgcacggcta
    11161 cgggatcatc ccggtcttcc tcgggttcgg gtgcaacgtg ccgggggccc tggccacccg
    11221 ggtgctggag acgaagaagc agcggttcat cgccgctacc ctcatgtcgc ttgcgatccc
    11281 gtgcatggcc cagattgcga tgatcttcgg ggtgctcggg ccgttcggcc tgcagtacgt
    11341 ggtgatggtc tttgcaaccc tcgcgctcgt ctacgtcgcc gtgggcctgg tcctgaacag
    11401 ggtggtgccg ggggagtgcc cggagatctt cctggagatc ccgccctacc ggatgccgga
    11461 cctccgggtc accgccacga agacctggat gcgggtgcgg gggttcatcg gcgaggcgat
    11521 cccctacctc ttcctcggca tcctgctcgt gaacctgctc tacgcggtcg ggttcctcga
    11581 ctggctggcg gccgtcttcg ccccggtgat ggagacctgg ctcgggcttc ccgccggagc
    11641 ggccgtcgcc ctcctggtcg ggttcctccg gaaggacctg gcggtgggga tgctggtgcc
    11701 cctgggaatg tcgccggaac agctcgtgat cgcggtgacg atgctgtcgg tctactttcc
    11761 gtgcgtggcg acgttcgtgg tgctcttgcg ggaactcggg gtgcggggga tgctcgccgc
    11821 cgcaggcgtg atggtgacga cggcgctcct cgtcgggggt gcgctccggg tggtgctgat
    11881 cgggttttag gagggagaag aggtgcgcag gaagacaaaa gaggagtaca ttgagatcat
    11941 atgcctgctc gagaagcagc gggggcgggc gcagaccggt atgatcgcgg aggcaatcga
    12001 cgtgaaacct ccgtcggtca cggagatgct ccggaaactg gaacacgagg ggctcatcgt
    12061 ctacgagacc tatgcaggcg caacgctgac cccgacgggg gaggagatgg cccgggacct
    12121 ccggaagcgg caccggacgt tcgcagacct gctggaactc ctgggcgtcg gcggggagac
    12181 cgcagaggcc gacgcctgcc agttcgagca ccacgtgagc ccggagacgc tggagcacct
    12241 cagggtcttc ttagcgttcc tcaaagagag cccggacggt cggcggtgcc tcgcggcgtt
    12301 cggggagttc cggaagcggc gaggatgagg gccgctcccg ggtcagacgt cctcccccac
    12361 cgccatgcag ggatacttct cccggggata cctcccctgg acggactcgc ggacgaactg
    12421 ctcccttgat gcggccgcga ggaggtcatc gtagacctct ttcggaacgc cggcgtagcg
    12481 gtaggcgtcg gcgctctcgc agatgatgag gagcgccttc gttgtggggt cataaccgat
    12541 ggaccgtatg ccgccggcgc gtccggctga ctgttgagcc ataggggtgg gttgtggtcg
    12601 gcggtggtga tagcggttgc ggcgggtacc gacgggagtc tcaccggacc ttctcgtagg
    12661 gatagcgcag cctgatgaac tccgatccaa acgtggcgat caaaccccta tgcgtccgga
    12721 ctgtgatacc gcgagtgagc agcagtgctg ttgccgcgaa gaacatggag taatatgcac
    12781 ggctcgcgga gtcctcataa aacccttctt ccagcagata ccgggcggca acgagcctct
    12841 gctccgattt ccggagcagc tcgtgaaccg tcgccttctc atccaacccg ggcaccttca
    12901 gtcagtaccg cctttaaaaa cggtgaatac cgccccctct ccatatcgtc gcgggaaagc
    12961 actttgaccg agaggttcac gccggtttcc agcataatgt cgtaggcgag cccgatcagg
    13021 ttccgcctga gatggaaatc ctccccactc gtgatcacca gcaggtcgac gtccgatgcc
    13081 gccgtcgctt cgccgcgtgc cgtcgagccg aagaggatca cctcctcgat acggccgccc
    13141 cactgctggg tggccctcgc aagaaacttg tcgagcgcag tgcgaaatga ggggtgcatg
    13201 cgtatcagtc cagtatcatc aggctgcgct atccctacga
//
LOCUS       DAJB01000145            6763 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_110614, whole genome shotgun sequence.
ACCESSION   DAJB01000145 DAJB01000000
VERSION     DAJB01000145.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_110614"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1234 a         2116 c         2189 g         1224 t
ORIGIN      
        1 ccttaaagaa cctctaaccc attttttgcc gggaatggtc ccgggtgcgc aacgcgtatt
       61 gactgcgagg agaagggacg gctatggctc ccggatgccg cctcgcgatc gccgttcttc
      121 tcgccaccct cctcgccatg acacctgtcc ttgctcacgt ccccctcttt gtccaggatg
      181 gttcctcgcc ggagtcggcg ttcgtcattg aggaccctgc gaagtcatgg gtggtctatg
      241 acgggctgcc ggatggagga aggagagcgg atatacgcga cgctccaggt cccgcgtgcg
      301 ggcgggttcg tgcccggcat ggtgcttgcg gggccgggta tccagagcca gggcccggtc
      361 ccggagggcg acggcgcgat ggcagtgccg ggagaacttc ctgaacagcc ggagtacgag
      421 ccgttcaccc cgtcgaaact ctacccgctg gcccgggtgg atatggcagc gccggcagcc
      481 ggcgactaca ccctcgcggt ctacacgtcg ggagagggag gaaattatgc tctcgcgctc
      541 ggcttcgtcg agtcctacac gctcggagaa tggatccggg ttccgatcga cgtcgtcgcc
      601 atccaccgtc atgaggggca gcccctcctc ctgatcttcg caccgatgat cgccgtcctt
      661 gccgtaggag cggtcctgct gctccggcgg cgccgggccc tctccctctt cgcgctcgcc
      721 ggggcgaccg caggtctcct cttcatcggg agcggcgcca tgaccctgat gcagatggct
      781 atcgcggctg tggggacgga acccggggcc gccctcctcc tgaccctggt cttcgcgctc
      841 atcgctattc tcctcggggt cctggcgctc cgggtggcct tccgggagcg gatcggagcc
      901 ggggagcgga tcgtcatggt ggcgctcggc gctctcgccc tcgtcacctg ggcggggctc
      961 gtgattggcc ccctgttcgc catcgtcgcc ggtatcctgc cggcacgccg ccggagaccg
     1021 ccgtaagtca ctcccggagc ggttcgagca tcgtgcgggc cgtatccagc aggctaccga
     1081 gcccctccgg tgtggcggtc cccttctcca tccagcggat gatgccgcgg ccgtccagga
     1141 ggtagatgta ggcaagcgag gggtcgtcga tcgcgtaagc ccgccggcac cgctcgatat
     1201 ccccgggtat cgtcagcacc cggtcctgct gcgccggaga gacgccgccg cgcaacccct
     1261 ggtggatccg gtcggcaagg gacccgccga tgaggctgtc gccgacgacc gcaacgaggt
     1321 aggtcgtcac ccgggaattc tcggtgaacg ccccggcaaa ggggccgctc caggactccg
     1381 ccatcggcca ggcggactcg aggaagacga ggacgacgag cgaggcgtcg cctctcgcat
     1441 cgtcgggaag cgtgacgacc ttcccggtga tcgacgtggc catgagggtg ggaaacgtcc
     1501 tgcctatggc tgcaggcacc ggctcggtgg ttgctgccgt tccattcatg ctctgccagt
     1561 gggatgcact cctgataaag cctctcccgg gaccggggat ctgtggccgt agcaaagagt
     1621 actataaccc tgaaccacaa cagttctctt acggaggctc atcgagcgtg atcagcatcg
     1681 gagagggatg gaaggatctc gtcgcggcgc ttgccgaagg cgacggaccg gagcgcgtct
     1741 acgtcgtcgg agcgacggac agcgggaaga cgacgctctg ccgctacctc gtcgacgaga
     1801 cggcaacgcg cacgaggacg gcatacgtcg actgcgacac cgggcagtcc cggatcggtc
     1861 cgccgacgac cgaggggatg gccctgtacc ccggcccgcc agagccctac ctccggttcg
     1921 tcggctcgac gtctccgggc ggccacttca tccagacgct cgcaggggca aagcgccttg
     1981 tcgagaaggc cgccgaacgt tcggccggcg ccaccatcat cgactcgccc gggctcgtct
     2041 ccggcggggt gggtgccgag ttccagttcc agatgatcga cctcttgcgt cccacccgga
     2101 tcgtcgccct ccagcacggg cgggaactgg agcggctgct cgcgaacttc gcccggcatc
     2161 ctgcgatggc ggtccaccgg atccccgtat cccctgcggc cgtcgcccgg accgcagccg
     2221 ggcggcggcg ctaccgcgag gaacgcttcg cagcctattt tgccgacgcg cgattgcagg
     2281 atatcccgct ccgggggctc gggctccagg gccgggtgcc cgacctgcaa aacccccggg
     2341 gggtcgaacg gcggctggtc tccttaaacg acccagacaa ctttacgctc accctcggca
     2401 tcgccgggga gatgcatccc ggagggcgcc ggctcgaagt ctttgctccg ccctttgatc
     2461 cgcgggccgt cgcgtcggtc aggttcggct cgatctccct ggacctcggg gccgaacccg
     2521 ggtcgatgga gtcctgccgg cgctgatcac gccgggaaga gagagaggaa gagcacgtac
     2581 accaccgtct gggcgacggt gagcgggatg ccgatccggg cgaactcccc taacgtcagc
     2641 gtctcgccgc ccttctctgc accctggatg atgatgacgt tgctcgccgc cccgaggatc
     2701 agcatgttcc cggcgatggt gctgccggcg gcaagcgcca tcatgcccac cgtggaggtc
     2761 ccgaggtgcg agaggaccgg gagggagagg gcgacgaagg ggacgttcga gacgaactgg
     2821 gagacgatga cgctcatcac ggcgatgacc gggaccgcgg tgagatcgag cgagctccct
     2881 tcaatgagcg gctggaagaa cccggactgc cagacgctcg ccataaggac gaagagaccg
     2941 gcgaagaagg ccagtgtcgg ccagtcgatc cggcggagga tctcgatccg ccgcctgctc
     3001 ccgacgagga tgggtatggc cgcaacgact gcgatccagg tcagccggat atcgagaccc
     3061 gggacgagca tgaccgcggc gaccttcgcc ccgatcagga ggacgagcag gatgagcgag
     3121 aggcgggcga gggccgcgag ggttgggtcg tggatctcct cctcgcggtg gaccagcggg
     3181 gcagttgcgt tgagctcgcc cgggaacgct ctcacgagga acccgtaggc gagcaggagg
     3241 gagatcgccg tcgggacggc gaggtagagg ggaaacgtga tgaacgggtt cgcaacaccg
     3301 cccgagagtg cgatgagcag gttctgcggg ttcccgatgg gacttgcaac gcttcccgtc
     3361 gtgacggcga acgcaagcgc gagcagcagg agttttgggg agatgccgtg ccgccgggcg
     3421 aagtagagca tcagcggggt gccgacgact gcgagcgtat cgttcatcag gagcgccgag
     3481 aggacgccgc cgccgatgag gatgagaatg agcaggtgcc ggaccgactc cgcccgggag
     3541 aagaggcgaa aggagaggtg gtagagatag ccgctctcgg cgagtgcttc cccgatcacg
     3601 aacatgaaga agaggaagag catcacgtcg aggttgatgg acgcgagcgc atcctccggc
     3661 gtgatcgctc ccgtcaccag gacggcggca gcaccaagga gcatcacctg ccagatgcgg
     3721 aagttgacgt tgcctatctt gcggaccgcg atcaggagaa agacgatgat gaggacgacg
     3781 atcgggatga gggtgctcat ggtatcgctg gcaggagaga tgcgcttgtc gagagtttgc
     3841 tctctatcgg aaaaaaggga tcgcgcgggt cctcaggccc cctctcctac ggtatagttg
     3901 atgacgctct cagaaatatt cccggagtac tccccgatcc gccggatact ctctgcgatc
     3961 tgccctgttg ggatcgccgc caccgcctcg aaccggagca tccgggcagt gatatcccgg
     4021 gtcatctcct caaggtcgcg caccctctgg agtgtcgcgt ttgccttctt gatatcgctc
     4081 gtgtaaaacg cctccatgct ccacgagaag atctccaggg caagcgcgct cgccttttcc
     4141 atgaggtcga gcgtcgccgc ttcggcctcg cggtcgatca gggcaagagc gttctgcgcc
     4201 acccgggtag tgtggtcggc tatccgctcg atgatcccgc tgacctggaa gtagtacgcc
     4261 gcccgggtta ccgtgatgtc catccggcgc gagagggcgg catcgatcat gatgaggtta
     4321 ttctgccgcg caacgagcca gtgcagccgg tccacttccg tcttccgcac gacggcattc
     4381 ccggcaagag ccgcatcgcg tccccgtacg gcggccatgg catcctgctg cataccccgc
     4441 gctaagagat gcatccgcct gatgatgttc tcaaacggca tctccacggg gttgaggaga
     4501 tccttgatgg ttatcgtggt gtccgtctcg ccgaccactt cctgcccgat cgccatctgc
     4561 gtgaactccc ggaccagctg caggacgaac ggcggcagcc ttcctctcga ttcaaggcgg
     4621 atggccgtga acccggcgat gtatgccccg acgaggaggc gaagcaggta ggtgcggtcc
     4681 gtcgcggcac cgacttcgaa gacccgggtc cggtgagtcg actccccgct gatctttggg
     4741 gtgatcagga gcgaaccgtc aggctgtact atcagtccca ccgggtcgtt cttctggatg
     4801 tttgcggact tgatccactg tttcggcagg gatacgatgt acgacgagcc cccggtgatc
     4861 tgaacctttc tgatctccat gaattgacac gcttttgtgc cgggagggta tatgcattct
     4921 cggttatata tatttcatcg aattttatcg gaggacaata tacccctata atcgcctaca
     4981 acgacaccta ttcgcacggc aagcatgggc cgggtgaggg ataggcgacc ggggtggtcg
     5041 cacgctaatt ccacatcagc cacgagatcc agagaagcag gacgatcagc agcacgatca
     5101 gtgcgaccga ccaggtcata tatcgttttg cgatggtctg accgcgggta tcccggggtt
     5161 tgatcttcag cacggtcacg gcgacgagcg ccccggctat cagtccgaca acctgcccgg
     5221 ccaccagcag cgcagtagtg atcaggctca tgggctggag caccagaaca aggccggcca
     5281 ccaccgtcgg cgggatgatg gcggcagcga tggcgacgcc ggcgatcacc tccgaagtgc
     5341 cccggttgaa cgccaccacc gctgcaaatc cgaggagcac cgccattatc gtatagatgg
     5401 ggttcaccac gaggcgtatg gcaatctcgt cggtcaatcc gagcggcatg acgatattga
     5461 tgagaaaggt ggcgattgcg gagaggacga agacacccag gagcagcgcc gccagcacgc
     5521 cgaggcaacg cagggcgttc tcgatcctgc cgagagcggc atagacggag aacccgtaga
     5581 tcgggcccat gatcggggag aggagcatcg ccccgatgat gatcgtctgg ttgtcgagga
     5641 gcaggccgga gagggccacc agtccggcga tggacgtcaa cgataatttt tcgagatcga
     5701 gttccccgta acccttaacc gtgccgagca gtttctcgac cggcgtcttc tcttcttttt
     5761 tctcgacctt cttctcagca cgcttcagga gcgatgagat gacgaactcc ggtgtcgata
     5821 cctcgatcat gttctcgttg taccgcaggt ccagcacgcc ctggatcttc tcgatcaggt
     5881 cgtcaaggtt ctcttccggc gtgtatacga taatctcata aacgtcctgc acgagcgata
     5941 tcgtgtagtg aaggccttcg agagtctctt ctacctgttt gtgcccgtct tcccggacgt
     6001 gtaccacgat ctttttcatg atccgaaacc atatttcgcc ggcatgagcc tccggtcagc
     6061 cggtccgcac tctggcctcg gggtcatgcg tagccgctga tctcgtagtc gatggccgtg
     6121 gaactcctga cgcactcttc gccatgcgcg aggaggtcct cgatcctctt ctcgatcatc
     6181 gccgggtaga ccgctcccac gagttcctca aagggccggc ccgagcagaa gaacttgaag
     6241 gtcggcgcgc cctggacgcc gtatcgctcg gcgatccacc tgttctcctc gatgttcagc
     6301 cttgcaaaga ggagcacctc gccgtagcgc tccgcaaacg tacggaagta cggctccatt
     6361 gtctggcagt gcgggcagcc cgggctgtag aacatcacca cagccggcct cgtccctttc
     6421 tcgacgacac tctcccagtc cagggccgtg acctcgacaa cagtagcgtt catctgaatg
     6481 atataacggt gatgggaaat ttaagttttt ccgagaacgg caggagagaa acgacgatcc
     6541 ggcaccggct ctccgtcccg ggggcagggt agccgggaag gggctgctgg agaaaccggt
     6601 gggacggcgt caactacccc ccgctaacgc agggggcttg cagcgggggc cccttgtggg
     6661 gcacagggct gcaagttaat cggtgcgaag gttgacggtt caaaagacgt ccttattcgc
     6721 acgggccggt tgacgcggcc cctaccgatt atccgtggga tca
//
LOCUS       DAJB01000146           10012 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_41621, whole genome shotgun sequence.
ACCESSION   DAJB01000146 DAJB01000000
VERSION     DAJB01000146.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_41621"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2493..2577
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8241..8332
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1733 a         3049 c         3046 g         2003 t
ORIGIN      
        1 tgctggcggc ggcgaacgcc tcctctctgt gctcagcgag tatagcaaga taggttatat
       61 cttctccggc atagagccgc cctttccggt ggtagaaccg tgcaccgatg atccccggga
      121 ctgcttccat ctctctatgg atgctctcgg tcagggcgtc gacggtctcg tcgaactcca
      181 ggtattcggt ccgctcggtg ccggtccact cccggacaat cccgttgaac gtgaggacgg
      241 cccctgcatg cgagacgccg cactcgcgcc ggagctcccg gaccagcccc tcgacggtat
      301 agtagtcctc gaactccggt agtgcggcga tcacctcgtc gatggtgggg ttccggagca
      361 cgacgttctc gcctggaagg tcgcctatca ctaccctcgg gaacggccgg gacttgaacc
      421 cctcgagtat ggcatattca acgccggtgt tgcagaggat tgccagggtg gagtcgaggt
      481 cgttctcgcg cctgacgatg accgacttct cttcgtcgac gccgccggat atagcagcat
      541 gcgattcata gtatagcgtg gtgtcttttc cgggctcgag tagaaatccg tggtgcccca
      601 gatgcttgac cgctccgacg gtcccgcgtg cggagagcgc cccgatcagg cttcttatga
      661 atgtggtctt cccggtgttt gagcggccga caatctgaat gatcttcata cgtcctcatc
      721 cctctctgtt attgggttct cctctccttc ctggccgggt ctggacttta agatgaccga
      781 cgccgcctcg gccacgcccc gcatcacctc ggtgatccgg tcctcgacgt cgatctcgtc
      841 gtcgaggttc aggctcgtcc cttccacctc cagcaggaac cgggcgactt cgcttgcctc
      901 gaagaggatc tcgtcgagtt cttctgcggt aaaaaagccg cacatggccc cgtagaagaa
      961 cgtggctcct gctttcttca ccttcttctt gaacgagctc cgcgcctgat tgaccgcctg
     1021 cggggtgtag gagtcgacca tgaacgggca gagttcgggg aggttctcac cgatgaacgt
     1081 catctccgag ccgctggtgg tcttgaaatc tgcgcagagg cgtgcgatcg cccactcgcg
     1141 ggcggtgata tgggtgtgct tgcgcaggaa ctggttgacc cgctggtagg tggcgccgtc
     1201 tactttcttg aatttctggt acttatttgg gtctttgatg ctttcaggct cttccatttt
     1261 acctatcctc ttgttggttt tgatatttca attgggctgg tctaacgacg gcgccggggc
     1321 ggctaagatg gcggttgaga gaatcgggtt tgccgcgggg gtccggttgt gcgcaggctc
     1381 cggaggtttc gcggctgccc ctgcctcagt taacgtatag cattatgtgg tttataactg
     1441 tctctttgat cccggagccg gggtgaggat ttggccgccc tggatccggt gttgccttaa
     1501 tcggggtaga atggttggtc ttcgggcccg ggnncccggg gcggcacggg ttgggcatcc
     1561 ttgccgggat gatcctgtga cggggattct ccggctatgg ggggcggtga cgttctgtag
     1621 cggcaacgga tgcgggcgag atgggggggc gatgttctct ggaatggctg gcgggagttc
     1681 caggaaggca ttgggctttg ggttgcgaat gcaggctcga tcgccgttcg gtaagggatg
     1741 ctatgggtgg aatgcgaccg gctcgtggga tgggggcggg ggcaggaggc accggtaggt
     1801 gggagttgcg actgacgaac gtccggagct tgaggggtca tcaggcttcg aggtttccct
     1861 cagatcctcg tgagcttcag tgggtgttgt ataatggctc cgctggggca ggtcgcccgg
     1921 gttttggcac ctccgtatca tggttttggg ccaggatcgc gggctccagc atatgaatgg
     1981 ttgtcggcgg ggacgatctg cggcctatgc ccttatgtgg gggcccttta tatgtgggcg
     2041 gggcctgcag gcttcgtggc accgatggta ctcctggatc gttcactttc tccccgcacg
     2101 gtatggggtt catatactcg gaagaggacg tattcaacag ggtctgttac ccgtccaccc
     2161 gggatggcag ccccatacct cctaactccg atgcctcctg catcaccgga aatattcttt
     2221 tttagggtct ggaacgaaat tgagttcctc cctggtttcc gggcggtttg ctggatgtat
     2281 tgtctccgaa gggatcggtc ggggctcctc tcgccgggaa ccccgggttg cccggggtca
     2341 cggaacggcg ccggaaatgg ccgatctctt gttttcgtta gatgaaccgg ggcgtgaaaa
     2401 gcaccttttc tgttcctccc cgaccgactt ctccttcctg cgtctcctgg tgcgaatacg
     2461 tggccggatc tccgtgttct atggcctttg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg
     2581 tccaatgggg gctctccggg tgaaaatcgt cttgcgggga ataggcattt accgcaattt
     2641 tgatatgctt cgttattcta atcaataacc aatggaaaac accctcttct cagagtggct
     2701 ggatcgcttc ggcaaccacc tccggatgcg gaattactcc ccgaggacca tcaaaaagta
     2761 cgggcagacc gttcagcgct tcgcccggta tgcctggctc cggcagcatt cggatccggg
     2821 cagggtttcg ttcgatgagg ccgcgttcga gaacgcttcg ctggatgcgg atgtcaacgt
     2881 ctctgcggcc ctcgtcaccg atttcttctc ctgtatttcg gaagggcggg agtataagcc
     2941 caagactctc caccggatga tatcgacgct ctcttcgttc tatcggtatc tctacgcaca
     3001 gggggtcgtg gttgccgacc cgatgctcgg ggtggagcgg ccccggatca agaatcagga
     3061 actgaaatac ctcaagcaca gccaggttct ccgcctgatt aacacgatcg agagcgagcg
     3121 ggatcggttg atcgttcgcc ttatctacgc caccggcgtc cgtgtttcgg agctctgcgc
     3181 gataaacgta gaagatatcg acttcgagga gcagacgatc cgggtcaagg ggaaaggcga
     3241 caagatcagg actgtcttcg tcgacgaaga gacgcttgag gagatcaacc ggttcatcgg
     3301 caatatgatc atggggccgc tctttctggg tcagcagggc aaccacatct ctccccggac
     3361 catccagcat ctcttcaagc aatacgcccc tgccgggatt acgccgcata aaatccggca
     3421 ttcctacgcg agcgagctct atcgccggtc gaagaacctc cgtgtcgtgc aggagaacct
     3481 ggggcactcc tctatcaaga cgaccgagat ttacctgcat accgacattg atgagcgcaa
     3541 acgtgtctat cagcagtttt tcccgctctc gaacgggaag gggggtgagt gatccgggnn
     3601 ggggccgtac ccttccggcc atcccgtcgg ccccacctct ccggcaacgc tcaaatcact
     3661 ggagtgccaa tcgtactcat atgcgctaca tcgtcacagg cggagcgggg ttcatcggct
     3721 ccaacctcgc agagcgactg gcacgcgacg accacgatgt cgtgatcatc gacgacctcg
     3781 tcaccgggcg gcgtgagaat atcgagcacc tcatcagcca tccccgggtg acgttcatcg
     3841 aggggagcgt cagcgacctc gccctgctca ccgatgcctt ttccggggca gacggcatct
     3901 tccaccaggc agccatcgcc tcggtccccc gatccgtggc aaagcccctg gagacaaacg
     3961 cggtgaacgt ggccggaacc gtgaacatcc tctgggccgc aaaagagtgc ggcgttccgg
     4021 ccgtcgtggc ggcttccaca tcggcgatct acggtgacga cccggctttc cccaaacgcg
     4081 agacgatggc gccgacaccg ctctccccct atgcggtctc aaaactcgcg ggcgagtact
     4141 atggaaaggt cttctcggac ctctatggta tccggacggt cttcctccgc tacttcaatg
     4201 tcttcggtcc ccggcaggac ccgaactccg agtatgcggc ggtgatcccg aagttcatca
     4261 cccgcctgct cgatgggaag ccaccgatca tctacggcga cggggagcag acccgcgact
     4321 tcatcttcgt ggcggatgtc gtccgggcga acatccaggc gatggagagc aacgcctccg
     4381 gtgtcttcaa catcgccggg ggcagcagga tcagcctcaa cgaacttgcc gggagtctct
     4441 cggagatcac cggggtacac caccggccgg tgtatgagcc gccgcggtcg ggggatgtgc
     4501 gtgactcgct tgccgacata tcccgggccg ggaaagcctt cggtttctct ccccgccgca
     4561 cgctcgctga ggggctccgc gagacggtgg cgtggttccg ggatgccggc cagtaaccgc
     4621 tccctgatcc ggagaccggc gccgggcacc ccggctgccg atcgcgggtt gccccgactt
     4681 cgggcatgag atctgcagga acttatatat cgtcactctg cagtaagggg tgcgaggtgc
     4741 caacgtatgc cccaattaat tacaccggag atcccccgta agggtgatga tagagatgcg
     4801 tcacgggcgt acaccgacgc aatcgtggag accctggacc ccgcggccca caacaacagg
     4861 ggtgtggcgc ttgagaggca gggacgttat gatgaggcgc tgactgcgtt cagtgcggcg
     4921 ctcctctccg acaacgagga cgtctacgcc tggaacaacc gggcagtgat cctcacccgg
     4981 ctcggccgcc cccaggaagc ggtgcttgcg tgcaggcagg cgctcgcgat cgaccggtcg
     5041 tgcatctttg cctgggttac ctacggcatg gtgctcggca ggctcgggaa ttaccaggag
     5101 gccgaacggg tccttgcggt ggcagaagac ctcgacccgc aggcccgtgc gctctacccc
     5161 gggaattcga cgatgacccg ggcctgaagc agggtactca ttttttggaa accttttttg
     5221 gaaaccgggc ggagtggtcg acaggcccgc cctatccccg ggtgcccggg gtccggggat
     5281 cgatacaccc tccggtgcta tacgggtggg tggtttgatg ggtctactca tgttcctgct
     5341 ccagccactg gccccgtatg catcccacga ccgccggagt tccccctggc cccccgggag
     5401 acccgtacgt ttataacctc cttgtcgcat tagagtgtgc caccgagtgc ccctccggca
     5461 cggagtcgat cccatgaaga cgctcattct tgcaggcggg agcggcaccc gcctcttccc
     5521 gctctcgagg gaacactacc cgaaacagtt catcccgctg atcgacgacg agtctctctt
     5581 ccagaagacc gtgaagaggt cgctcctctt ctcctccccg caggagatcg ccatcgtcac
     5641 caacacggac cacaaatttc tggtccggga ccagctcgcc gccatcgggt gcgactgccg
     5701 ggtcctcgtg gagccggcag gcaggaacac cctgccggcg atctactacg gcgtcaggga
     5761 ggtcgccggg ggggacggat ccccccagac ggtcgcggtg ctgccctcgg accacctgat
     5821 caccgcgaac gggccgtttc acgaggcctt ccggcgggcg gagcggctcg cgaaggacca
     5881 cctggtagtc ttcggggtgc ggccgaccct cccccacacc ggctacggct acatccgtcc
     5941 gggcgagccc gtgacggacg gttcgctcgt ggacgccttc gtggagaagc ccgaccttgc
     6001 gaccgccgag aagtatgtcg cggacggcta cctctggaac tccggcatgt tctgcttcga
     6061 cgccgatctc ttcctcgccg agtgcgaggc gtgcgcaccg gaggtggccc gggcgttcga
     6121 gcagcccgtc gaggaggcat acggtgcaac ccctgccctc tccatcgact acgggatcat
     6181 ggagaagacc cggcgggcgg cggtcgtgcc gctcgaatcc gactggagcg atgtcggaaa
     6241 cttcgacgct ctctacgccg cactgccgaa gaacggagac gcgaacgccg tacggggcga
     6301 gcatatcggg atcgactcct ccgagaacct gatcatcgcc gaccggttga tcgccaccgt
     6361 cgggattcac aaccttgcca tcgtcgagac gaaggacgcg atcctggtcg ctgcccggga
     6421 cgaggcccag cgggtgggcg agatcgcgaa agccctccgt gagaaagggg actcccgtgc
     6481 gctcttccac acgcaggtcc accggccgtg gggctcctac acgaacctcg aggaggggca
     6541 gtcgtataag atcaagcggg tcaccgtccc cccgaaacgc cgcctctccc tccagatgca
     6601 ccaccaccgg tccgagcact gggtggtcgt caccgggtgc gccgaggtga cgattgccga
     6661 tgcgacctac ctcctccgga acggcgagtc caccttcgtc ccggcgggaa cggtccaccg
     6721 gctcgcgaac cccggcctcc tgccgcttga attgattgag gtccagatag gggagtacac
     6781 cggcgaggac gacatcgtcc ggttcgagga cgacttcgag cggacgtgag tgcgggtcgg
     6841 cggtccaccg cccctctccg gctccgcgtc tttccgcgac caaaccgata taatcctaca
     6901 atgcatagaa tataagagcc gataccgcag gtatcagtcg taggttcatt tctgtcaccc
     6961 ccgtagtgta gtggtcaatc atgcaggact ttggatccgg cgacggcggt tcgaatccgc
     7021 ccgggggtat ttttacgcgg ggaggctgaa caactgcggc cgaccccccg ctcaaacagc
     7081 cgcaccatct ctctgccaca atcccgccag tccggggtta tacaatgcaa cgggcgcgag
     7141 cggcacaact ctttttctcc agagccctgc atactccttc cgggcgtcgt acgatcagat
     7201 gcaggcgatg aagcccgcct caaaccgccg ataccttttt tgcgctcgat accctcccgc
     7261 ctcccggtgc catagagcat gactgaaatt gacacgagga tacgatcaat ccatgcccgt
     7321 gagatactgg actccccggg gaacccgacc gtcgaagcgg atgtcacact cgccggcggg
     7381 gcgtccgggc gtgctgcgac cccgtccggc gcatcgaccg gtaaacacga agccatcgag
     7441 ctccgggacg gcgtaaaaga ccgttacggc ggcaaagggg tgcagagacc cgtcgcccgg
     7501 gtcaacggcg agatcgccgt taccctgcag ggcatggacg cccgtgatca ggcggcggtg
     7561 gacgaggcgc tcatcgatct cgacggaaca ccgaacacgc ggaacctggg cgccaacgcc
     7621 acgcttgcgg tctccatggc cgtcgcccgg gctgccgccg ccgcagaggg tctctggctc
     7681 tgggagtacc tgggagaccc gttaaagccg ctccttcccg tccccatgat gaacatcctc
     7741 aacggtggag tccatgccaa ctggcagggg tcggatttcc aggagtacat gatcgccccg
     7801 tacggcgctc caggcgtcgc agaagcggtc aggtggggaa gcgagactta ccacgcgctc
     7861 aaagagatcc tcaaagcgcg gggctacacg accggcgtcg gcgacgaagg cgggtttgcc
     7921 cctgcggtct ccacgaacac cgagccgctc gacctgatcg tggaagcgat cgagcgggca
     7981 ggctatgtcc ccggcaaaga gatcggtatc gctctcgacc cggcatcgag cgcgttctat
     8041 aacaacggcg tctatagcct caggaccgag gggctgaccc tgaccgctgc tgagatggtg
     8101 gaccggtacc gtgaccttgt tgcggcatac cccatcgtcg tcatcgaaga cggccttgcc
     8161 gaggacgact gggatggctg gcgcctgctg accgggacgc tcggggaccg ggtagaactg
     8221 gtcggcgacg acctcttcgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcatcgc
     8341 cgatttcacc gtcgccatgc agacgggcca cctcaagacc ggcgcaccgg cccggggcga
     8401 gcgggtggag aagtacaatc aactcatgcg gatcgaagaa gcagccgggg atgcggcccg
     8461 gtatgccggg cggagcgggt ttgtgcgata agatagggta cggcgtcggc gactgaggtc
     8521 ctcaagcggg ctatcctgca gtagtcggag caggcccgtg ccggcattgc cgccggcagc
     8581 ggcctgcaag aggaacccgc ccgcgcgaca gcggatagtg aaagagccct gcaactctcc
     8641 cgggttccgt acgattttta aggcctcgcc ccccattacc ctgagtacca atggacgccc
     8701 tagcaattga aatccagatg agtctcctcc tcttcctcgc actcgccggc tacctcgtgg
     8761 catcccggat caatcagtcg gcgacgatcg gggccattct cgtgggagtc ctggtcggac
     8821 cgagcgtgct cggcctgatc acctacacgg actttgtcgc gagtctggcc cacctggggg
     8881 cgatcatcct cctcttcgtc atcggtttcg agtttgatat cagggatatc ctcgatgtac
     8941 gctacggcgt catcgcgctc atcggtgtag tcgtcccgtg gatcggcggc tacgccacca
     9001 ccgtcttctt cgggttcgac ttcgccagcg cagtcttcgt cggcacggcg ctgaccgcga
     9061 cgagtatcgc catcacggca aacgtcctca aagagatcgg cgtgctccag accggggcag
     9121 cgagggcaat catcggcgcc gcagtcatcg acgacgtcct ctccctcctg gtgcttgcca
     9181 tcgccaccga cctggtagtg agcggggatg tctcggcggt atcggtcggc gtgatgctcg
     9241 ccaaggccgt gggattcatc gtcgtcgccg gcgctgtcgg gtatttcggc atacgaaagt
     9301 tcatccagcg gatggacgca acgtccctcg ccgggaagta tcccgagttc gtcttcatct
     9361 ttgccatgat gatggcgttc ctctacgcga tgctcgcaag cctcgttggc ctatcaggga
     9421 tcatcggtgc gttcctcgcc ggggttgtct ttgccgatgt cgagctccgg cagagcaagg
     9481 gtgtcaagga gggtgccgag tacttccaga tcgtcttcgc ctcgatcttc ttcgtatcgc
     9541 tcggaatcct cgcggacgtc cgtgcactca cctccgacat ggccctcttc ctggtggtgc
     9601 tgacgctggt cgccatcgtc accaaggtcg tcgggtgcgg cctgcccgcc cgggcgatgg
     9661 ggatgtgccg ggaggactcc ctcatcatcg ggttcgggat ggcgccgcgg ggcgaggtgg
     9721 cgatgatcgt cgcgttgatc gggcttgatt cggggctcat cggccagggg atctttgtgg
     9781 tggtcgtcct gatgagcctg ctgaccacca tcgtcacccc gatcgtctac cggagctggt
     9841 tcttcaaggg cgcgtactgt gccacggagt gatgcagggc ttccccctgc agtttttccc
     9901 aaaaacggct ttgttgaacc cccacttgtt tgctccaggt ggtcgcaatc aggggtactc
     9961 aggggcacgg ctttccattg agtatcgcca tgactgcccc cgccccctgg gg
//
LOCUS       DAJB01000147            4877 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_128915, whole genome shotgun sequence.
ACCESSION   DAJB01000147 DAJB01000000
VERSION     DAJB01000147.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_128915"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          900 a         1563 c         1544 g          870 t
ORIGIN      
        1 gaaagaacca tccggtcctc aggatactct ttcagatcgt gaatttccac ggctggaaga
       61 cgcacgggca ctgggagacc ctcaaggact ggccgggtat gaagagggtc gaccggatcg
      121 cgatcgtcgg cggggagaag tgggaggagt ggatgaacca cctgccgggg ctctttgtcg
      181 gctttgtcgg ggtcgatgtg cggtacttca ccgaaggcca cctggacggc gccctcgact
      241 ggctgcggga gccgatagcc tccgaaggga ctgtcgttga ggtgcaatag ccccgccgtg
      301 gtgcgagaga ccgcctcccg ggagggcagg caaccctttt ttaccccggg cagcctaccg
      361 gtgatcagac gaatacgaat ctcatgcact attataaaga tatcatattc gaaaagcccc
      421 tgcccgagga gatccggcgt cttgggctgc tcccggcgga cctccatttc cacacccggc
      481 actccgactc ggccacacgg gtacgggatg cgttgaaact cgcggcccgc cggagagtcg
      541 ggcttgcgat caccgaccac aaccaggtcg gcggcgtggc cgatgcagag cggcagacga
      601 tccacgtccc cctcattccc gggatcgagg tcagcgccaa cgacggcccg catatcctcc
      661 tctacttcta ctcggcctcc gaccttctgg acttctaccg gtgtcacgtc gagaagaacc
      721 ggaggggcgg cccgttcacc gcgatacgcc tggacacccc cgacattctc gaccgccggg
      781 aggattactc ctgcatcgcc gtcgaggcgc acccctgcgg ctacgccttc ttaaaccggg
      841 gggtccagcg gtgcatcgcc ggcgcgtgca ccggcgaaga ggtcttctcc cgcctcgacg
      901 ctctcgaggt gatctgcggc gggatggccc ggtcccataa cctgaaggcg gccgggctcg
      961 ccgtcgccca cggcctcggg cggaccggcg ggaccgacgg ccacctcctc cacgaactcg
     1021 gcggggtcgt cacctgcgcc gaggccgata ccgtcgagga actcctcgac gccatccggg
     1081 cgaggaagac cgtcatcatc ggtcgtgagc ggccgctcgt cgagaaggcg gtgatgggaa
     1141 ccgcggtcct cccgcaccac ctcccctaca ccgtccctat cctccaggcc cgctgggaac
     1201 agagcctccc ctggctcagg aggttcgtcc gggcccggct gaacgggccg gcctcccctc
     1261 ccccggaaga tgagaagagc cgggagaccg ggcggggtgg cgcccggtag agacggtgtg
     1321 gttctcctcg aagatctccc ggaagacccc cgggccctcc cgctcctcga tggcgaacat
     1381 cctctgccgg gcgtagttct gcccgaagag caggtagagc acaaacccga ccggtggcag
     1441 gaagaagagg accgtcagcc ccgcggtgga catctccggc gaaccgccgt atattcacgc
     1501 ggagaaagag gcgcggatgc tggtggcccg cgggtgaagt gaagaatatg atgcggagag
     1561 gatccgcgga tagtccgggc agccatagcc ttttcccttc agaagtagat agagggagcc
     1621 atgcttgacc ggatgaagga aagttcagga agtgttctcg ggttccggtt tgatgggaag
     1681 ctgagtgaga gcgattacgc caccatcctg atccccgagc tcgagcgagc gatggaagag
     1741 cacgagaacg tccggatcct cttaaagatc gagagcttcc ggagctggag gccgggtgag
     1801 ggctgggacg ccttcaaaca gtggccgggt atggagaggg tcgaccggat cgccgtcgtc
     1861 ggcggagagc ggtggcggga atggatgaac cacctgcccg ggctctttgt cggctttgtc
     1921 ggggtcgacg tgcgatactt cccggagaac cgcctccggg acgcctgggg ctggctccgg
     1981 gaggggcttc tagtcccacc gcaaccggca tgaaacgcct atatatcccg gacgccaatc
     2041 ctcgggtatg cattgtccgg tctgcgggca cgactgcgtc acggacgccc gtgcactcct
     2101 cacggaactc cccgggcgtt tcgccccgtg tcccgactgt atggggctcg tctatgacaa
     2161 gcagagccca ccgcccgata tcgataccgt ggagccctgc ccttcctgcg ggaagcggtt
     2221 catcgacgaa gtctttgccc ggatctaccg ggtgatggtc gctgaaggag accttgccgg
     2281 gaccgaaccc ctcgcggccg tcgggacgcc gctcgttcac cccgggtctg cgatgcacag
     2341 agccccctac ctcccgccgc gatcgctcgt cctcctctcc cggtcggtcg gagagcgggc
     2401 cgctgcccgc ctggtcgccg aggtcccgga ggtccgtggc gtcgtccgga cgggtgccgc
     2461 gacgccgggg atcaaagata ccgacgcggc accgcaggta tacaccctcc tcgccggctg
     2521 cgacgtccgg gccgacattt ttcacacccg cgcgggaccc atcgtcgttt acaagcagca
     2581 gtcggccctg catatcgagt ttccccggga ccgcgatcca aagatcctcg ctctcgagcg
     2641 ggagatcggg cggcatcggc cccggacctt cgttgacgcc tgctccggcg ccggcaccct
     2701 cggccttgcc gccatccggg cgggcgtccc gcacgtgatt gcgaacgacg cctggtatgc
     2761 ggcggcatac tggaccgcat gcaacctcca ggtgaaccgg gagttcctcg ggatcgggga
     2821 ggtgacgatg caccgttcct acgacgatct ccgtcgccgg ccggtcgcgc gggacccaat
     2881 ccaggtcgcg acagccgccg gcacgcagga ggctcatgtc taccagggcg atctccggct
     2941 gctcccgacc gtcctcccgg ccgaagtcga ccttgccgcc ctcgacctct tcgagaaggc
     3001 ggacgccggg aagatcgagg agatcacccg ggcctggcgt gcccgggtgg gcggagctat
     3061 ttttatccca tagaccccag tatatatggg gactagcctg ggtggctcgg cgtcacctgt
     3121 tacccgaaac cgccgttagc ggggggcgaa gtctgaggaa ggccacagcg gcctgcagga
     3181 tccactgcgt cccgacggag aagcctcgtc ccatgaggtc ggcggagagg tatcaaagcc
     3241 ccggaggggg tggcgacctt ttgccgcggg taccggttca ggcccggaag ggagcagact
     3301 taccgtggac gttcggcgcc catggggttg cggggtggag gagggatgca gtggtgaatg
     3361 cctgtgcgca cggtcgaccg aggacgtgtc cccaacggcg atctccatgg caaaagtaac
     3421 gattcttggg gctacaggga acgtcggcat atttgcggcc cacaccatat ccgagatccc
     3481 gtacgtcagc gacatgctgc tcgtcgggag acccgggcgt gaaaattttc tcgcgggctg
     3541 ctgccgtgac ctttccgatt catttgctgc ccgaggtacc gacatccggc tctcgtacag
     3601 caccgatctc gcagatacaa aagactcgga tattatcatc tgtacagcgg gagtgccccg
     3661 tcagcccggc caggacagaa acgacctcgc cttcgagaac gcgaaactcg tcgccgcgac
     3721 cgccgagacg atcggccggt cttcgcccga tgctatcctc cttctcgtga caaatcccgt
     3781 cgacgtcacg accgccgtcg ccctgaagta ttcggggttc cagccaaggc atgtcttcgg
     3841 cctcgggacg cacctggact cgatgcgctt gaagtccctg attgcacgat acttccgtgt
     3901 ccacgtcagt gaggtgcata cccgtatcat cggcgagcac ggcgacagca tggtcccgct
     3961 ctggtcggcg acaacgatcg gcggcatccg gatcaacaac ctgcccacct tctcgggact
     4021 gcccgtgcag gagatgatcg acaccgtcag gacgagcggc gaggcgatca tcagggataa
     4081 gggcgctacc gtctacgggc ccggagaagc gattgcaacc cttgtgaaga cgatcctcgg
     4141 gaacgagaac cgcatcctca ccgtctcaag ctacatcaaa agcgagatcc atgggatcgg
     4201 cgacgtctgc atcggcgtgc ccgcccgcat caaccgtgac ggggtcttcc cggttccgct
     4261 caacctcgaa gaggacgaag tcgccggatt ccgcgagtcg gtcaaaaaga tacgcaggat
     4321 cactgctgaa gtgatggagc ggcttgaaga ggctcgttag ggcagaaaat gggtatggga
     4381 atccacttat acgagagtgg attcgatgat ctcagcgctt ccgacgcctt caagcttctg
     4441 gagcgctgct tcgatctcat cgggagcccc cgctttgtcg gggacgacga tggcggcttt
     4501 cagtgccacg agaccgaagc caatcggctc ttctctgata tcgtcgaccg ggacagccgc
     4561 cttgacggcg gctttgagtg cttcacggtc gacatcgggg gattcgggca ttatctttac
     4621 aatgatggct acgtttccca tcggttatgg cccccggaac ccacacttcg gacatgtata
     4681 tgctatgctc tgctccctgc accggtagca cctgttgatc tcgtgcgcac acacggggca
     4741 cgggaaccag gtggagcctt cctcggccag cgtggcgttg caggaggtac atcgatctct
     4801 tgctgtcatc gttatttcct cgaatgttaa cctatcatat cgtctctgca gcaagtgaac
     4861 taccccgccc tgaaggg
//
LOCUS       DAJB01000148            6501 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_72799, whole genome shotgun sequence.
ACCESSION   DAJB01000148 DAJB01000000
VERSION     DAJB01000148.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_72799"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    6144..6158
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1391 a         1940 c         1926 g         1229 t
ORIGIN      
        1 aatcgctacg ggaggttagg gtacaacgct ctgcataaaa gcctccgctt taattattaa
       61 attatccccc gacggatata cctttctcta ttataacagt agaaatgcga tattaaacgt
      121 aattcccggg agaaatccgg tatattcctg ttcagcgaca gagtttgcgt gttgttcccg
      181 gttggtcccc gcagaaacgc gcccatgcac gacctcccgg cataccgtat cctcatggtc
      241 gaagaggggt tgaaaacagt tcgtatcgga acggggcggg gaaccccacg ggagcctcgg
      301 agaagccggg ttgtggcgct gctgcccggt aatcccaccg gtggcgtctg agggcaggtc
      361 tggagagccc aaaagacggc gagcaggtat ccgggaaact gtgccggaga acaactgttc
      421 gtaatacggt ccggtgagtt tttttgcagg ataactataa aaccagaacc tgcatgggga
      481 gagtcctgcc atggcaccac tgcccgagaa catccgtata ctcatcatcg gcgccctgct
      541 cggggtatcc gtctttctga cctattactt tcatgcagtc ctctcggtcg gggccgtctt
      601 ctcccacttc ttctacatac ccatcatcct cgctgcgctc tggtggggga gaaagggcat
      661 cagcgtggcg ttcttccttg gcgggctggt cctgataagc cattacctcc tcaggataga
      721 cgtcgggacc ttcaacgact acgggcgtgc gctgatgttc gtcgtggtcg cagtcgtcat
      781 cgccacgctc tccgagcggt taaaaaggca gcagaaggac accgagagag agcgtgaccg
      841 ggcccagggc tacctggacg tggctgccgt tctgctcgtc gtcatcaacg cggaccagac
      901 cgtcggcctc atcaaccgcc gtggctgcga gctgctcggc taccgcgagg aggaactgct
      961 cgggaagaac tggtttgatg tagcggtccc ggagcgcaac agggaggacg cccggcgggc
     1021 attcacccgc atgatatccg gggtctccgc ggtatccgct tcggatgaga acgtcatcgt
     1081 gacggggagt ggcgatgagc gcacactcat ctggaaggag gccgttttac gggacaccga
     1141 tggccgggtc accgggatcc tcgggtcggg gaaggacatc accgaccgga tccgggtgga
     1201 ggaggcgctc cggaacagcg aggcccagct ccgcacgatc gtcgagaacc tgaacgaagg
     1261 gctgggcgtc ttcgacctcg acggcaaccc gctccactgg aaccgtgccg cccgggatat
     1321 ctacgagccg tccggccttg agattgtccc cgggaatgca gaggatctcg gtgaaatatt
     1381 cgagatcgca acgatggagg gtaccgccct tccggtggag gagtggcccc tctcccgcat
     1441 cctccgcggg gagcagctcc gggacatgga agtgcgtacc cggcgcatcg ggaccgactg
     1501 gcagcgcata ttcaactacg gcggcaccct cgtccgcgac gcggacggca acctgttcat
     1561 ggcgatgctc acgttcaccg acatcaccga acgcaaagag gcggaagaag ccatcatgac
     1621 ggcgaacgag gaggcgaacc tctacctgga catcatggtc cacgacatca acaacgtcaa
     1681 taccgtggca ctcgggtaca gcgacatcct cagcgaagat ctggagggaa aggaccggga
     1741 ggtagcccgg aagatccggt cttccgtccg ccagagcatc gagatcatcc agaacgtctc
     1801 cacgatccgg cgtctccgcc ccccaaaccc cgtcctgaaa cctgtcgatc tcgacaccgt
     1861 catccgggcc gagctgcggc agcacccgga gaccaccatc cggtacagcg gcacgtcggt
     1921 cgacgtcagg gccgacgacc tcctttctga ggtcttcacg aacctcctcg gcaacagcat
     1981 caagttcggg gagcccgatg tcgtggtcac gatccgggtc gaggagcagg acggggaagt
     2041 gctggtctcg gtcgaggaca ccggcccggg catccccgac gcgataaagc ccaccctctt
     2101 ctcgaggttc cggaagggga agagttcgag gagcggcaag ggtctcgggc tttacatcgt
     2161 gcggatgctt gtcgagcggt atggagggag cgtccgggtg gaagaccggg tgcccggccg
     2221 gccggaggaa ggggcggcgt tccggttcac gctcataaag gcgcaggaag agtgatcaga
     2281 tccgggcaga gagcccggac ggccccgtgg ttcccggctg cacgggccac agcgaggcat
     2341 ccgccggcat aaaaacggga aatttgtggt acatcccgga gaaaggtcct tcgaaataaa
     2401 aaggtttttt ttaccgatct gcatattctc tctactatga aacaccgtgc gatcaccgcc
     2461 ttcctggctg catcggccct tctcctcatc cttgcggcag gatgcaccgc cacaacagaa
     2521 gagaagaccg atcccggcgc tccttcgccg gccgacgagg cctacgtgaa ggggctcgcc
     2581 gggtacgcgg acgccaacta ccgggtcgcc gaagaacgct ttgcggaggc ctacgccctc
     2641 tacgccgacg ctccggataa agccagagcg gccagggacg ctatgttccg ggcgaaccgg
     2701 acggtcatgg agtacacgct cgacaccgag gccgcagagg cggcgctgcg ggagaaggtc
     2761 cccggcatca ccgatgagca gatagccgac tggctggaga accgcgccca gaagatcgtc
     2821 tctgaaaacg agacgctcta cttctacgac gtcgccggga actatctcta cgcgcacgtc
     2881 gacgagatgc ggaagcagaa cgggaggaat ctcgacttcg actacatcgg ccggtacgcc
     2941 tggtcggagg aacagcccga agggtccggg ccgtacgtga acccggtgca ttacgcgggg
     3001 accgaacggc ttgagatccc gctcgaagaa ctcccggaga cgggcatgct gaagatctgg
     3061 ttcccgctcc cggtcgagac ggactcgcag agaaacgtca ccgtcaccaa cctgacgtgt
     3121 ccggattaca tcgtcgcggg cccggtcacc tccgggggta tcggctacgt ctactacgag
     3181 atcccggccg gggcggtcaa cgggaacctc gttctctcgg cggatatcgc gttcaccgcc
     3241 cacgagcaga tctttgacga tatcgacccg gctaaggtcg gggagtacaa cacaagcgat
     3301 cctgagtacc tgctctatac cgtatccgaa cgcaacatcg agatcacgga tgctgtccgg
     3361 gagaaggcga aggagatagt cgggaacgag acaaacccgc atcttcaggc gcagatgatc
     3421 tactaccata tcatcgagac ctacccctac agccacgtcc cgcacggttc gctcgatagc
     3481 cgcgagccca aggtcgcgga gtccacgcac atgttcgaaa ccggccacgg cgactgcggc
     3541 acccagagca tgctctttgc cgcattctgc cggtcgctcg ggatccccgc ccgcgccatc
     3601 gggggctacc agatggtcat agccgaggct cccggcaccc acttctgggc ggagtattac
     3661 ctccccggct acggctgggt cccgaacgat cccaccattg ccgaggcggc cgactgggtc
     3721 cccatcaccg acgagaagcg gtcgacgttc aaggattact atgccacgaa cctcgatcct
     3781 gctcgcttca tcatccagaa gaacgtcgat gctccgatgg acccggccct cccggaagat
     3841 gccgccatct tcaggttggt caggcagtac ccggcgatcg tctccgacac ggcagactat
     3901 gatctggatc tcttctggac ggagtacttc agcgtcagcc ttgctgccgt cgactgacac
     3961 cccctctttt tagcaacagg cccttccata tcccggtgcc cgggtaacta cgggccgtgc
     4021 atcactaccc gtgtgagcca tgggctgatg cacggctgct gccgtccatc gaggcaaggt
     4081 tattgcacgg cagattcgcc gtggacgcaa ccggttttga tacgtctgct ggtcgtggat
     4141 cgagacggtt ttccctgaaa ccggggtatt ttcatcatat gttgataaaa tcccggaatc
     4201 agccagatat cggtcgagtt tactccggat gaacctgctg gaggtgaagc catcctgagt
     4261 atttatataa cccgaaaaaa acaggcgata tgatacggta gttgcgtgga tggcagagag
     4321 agtttttaac tgataatgcc tgttttttct accgaattta ataatcggca tgaatttttc
     4381 tgatggtaat accgtaatat ctatccgtag aacgattcgt caacgtatat atggatacgg
     4441 ctccatacaa ctggttaact gtctcacttc gaccgccaga tactgccgta tcacatcatg
     4501 actccgagaa acccgcttga cctgagaaag ttcgtagctc ctgagtttat ctgtggatac
     4561 ggagcactgc accttgcagg ccggtatgca cgcaacttcg ggatgaaaca ggtgctcctc
     4621 gtcaccgacc cgggcgtccg ggccgcaggc tgggcagagg ccgtcgggga gagcctgaca
     4681 gaagcagggg tatcgttcac cgtcttcgac tccatctccg tcaatccccg ctccaggcag
     4741 gtgatgtcgg gcatggaggt gtaccggtcc gaagagtgca acggcatcgt cgccgtcggg
     4801 ggaggaagtt cgatggactg cgccaaggga atcgggatag caagctccaa catgcgggat
     4861 atcctcacgt tcgagggcgt cgaccgggtg ctggtgcctg ccccgcctct catctgcatc
     4921 ccgacaactg ccgggagttc tgccgacgtc tcccagttcg ccatcatcac ggacgaagag
     4981 cgaaagcgaa agatcgccat catctccaag acacttgttc ccgacatctc tctgctcgat
     5041 ccccaaccgc tgacgacgat gtcttgggag ttgaccgtgg ataccggcat ggacgccctc
     5101 tgccacgcga tcgaggccta cgtctcgaac gccagttccc cgatgaccga catgcatgcc
     5161 cttgaggcga tacggctcat ccatgacgca ttgcctgctg tcatcctgga gccggaagac
     5221 ctgaacctgc ggttttcgac cctcctggcg agccttcacg ccggcctcgc gttctcaaac
     5281 gcaagcctcg gagccgtgca tgcgatggcg cacagtctcg gcggggtctg cgatcttgcg
     5341 cacgggcggt gcaacgcgct gctccttgag catgtgatcc cggccaacta cgaggcggca
     5401 tcggagcaat acgaccggat aggggcgatc atcggcccgg tacagggcga acgaacgcct
     5461 ccggcagagg gaatagcggc attcaggaga tcgcttggca tcaccgggac cctcgcctcg
     5521 gtcggtgttg gggaggacgc gatccagacc ctcgccagaa gggcgtatgc cgacccctgt
     5581 atggtaacga acccgcgcag gctcacctgc gaagatatcg aacggatcta cgcatatgcg
     5641 ctctgaacct cctgatgacc gggatatgct ccgggagaag atcatcggcc ttggagagac
     5701 gtctctccat aagagttact atccggagtt gcagagccgg cttgaggaac tcgaacggtt
     5761 cagggccctg ctcgaccaga cgcacgatgc aatatttctg atccggatgg agaccgggaa
     5821 acttgcggac gctaacgcgt ctgcggggat gtatctcggc tactcaaagg aggagctgct
     5881 tgcgataccg tttgccagcc tgttctcccc gcagcagaag gccagactct ctgagttcat
     5941 ccctgagagc agcgatcgtc catcagattc gcggcagacc tgcatcacat cgcttctatc
     6001 ccgggagggg acggagatcc cgatggagat caccgttcga tccgtcacgt tcggcggcga
     6061 gcagtatgtc gtagcgattg cacgggacat tacggaccgg atccgtaccg agagcgaact
     6121 caagataaag gagagcgctc ttgnnnnnnn nnnnnnnnag aaacctggag ggattttttg
     6181 cagatcccac cctcggcgaa aagatcacgg gtcatctcct tgataaccat accatcgtgg
     6241 acgagatgat cgggctgcgg gacaacggat ccccgtttca tatccaggtc tcgggcagcg
     6301 tcgtgcagaa caatacccgg cagccgctct gcatcatgct gatcgttatg gatatcaccg
     6361 agcggatact caccgaacag atgcgacggg agacatatgc gcagattgaa aagaatatcg
     6421 agcagtttgc tgttctcggg gaccatatcc ggaacccgct gcaggtgatc gtcggatatg
     6481 cggagatgat cgacgacccg c
//
LOCUS       DAJB01000149            9386 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_44824, whole genome shotgun sequence.
ACCESSION   DAJB01000149 DAJB01000000
VERSION     DAJB01000149.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_44824"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    173..285
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8440..8628
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1779 a         2886 c         2787 g         1630 t
ORIGIN      
        1 ctaatcgcta cgggaggtta ggataacacg gaacgtgtcg gcacaggtat ggatcccgtc
       61 ccgggacacc ggagtggagg aggcagcggg ccgtgcagac ccgcgggagg gagcgtacct
      121 ccccggaccg caaaaataag gaagtgtcta gcacgtcccg ccgcagccgc agnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgata tggccgaggt
      301 actcggtctt gagcaacgca cccgcgacgc agctgctgta gagaagcgcc gactcagcga
      361 gcggggccgg gaggggcgct gtcagcacga tatcggagac catgagcctg ccgccggggc
      421 ggaggacccg gaaggcctct gcaaacacct ccgctttatc gggagagaga ttgatgacgc
      481 agtttgagag gatcacgtcg accgaactgt ccgccaccgg caggtgctcg atctcgccga
      541 gacggaactc tacgttcgcc cgcccgctct tctttgcgtt ctcccgcgcc ttatcgagca
      601 tctcggccgt catgtcgacg ccgatgacgc ggcccgtcgg gccgacccgc tccgccgcga
      661 ggaaagaatc gaaccccgcn ngggttgccg cacccgagcc ccaggttcgc gccctccggc
      721 gcctgttcga ggtcttcttc cgaatagccg atgccgaggc tcaccgattc gaccgatctc
      781 cccgtgccgc agcacccggt gccgcatccg catccgccct gccgggccac ctgcccgtat
      841 gcactccgca cctgtttatg aatatcttct tctttcattg tgattgaact cctgagccgt
      901 ctgatgcgcc tttgtctgcg agctcctccg tcatgggaga gagcagcgtc gttaccatcc
      961 cgttgaaccg gctcgccttg atcagcgtga tgcagcagac gtctccttcc ttctcccaga
     1021 agacaactgc aagccggtcg ccgggagtga ttcccgcccg ttcgcggaga tctttcggaa
     1081 gcaccagctg tccccgtgcg tcaacgctca cgaccgcctc gatcgtgcag ggtccggaac
     1141 aggaggatct ttgctcatct ctggcatccc ccatgtggct cacgggatca tagttgtctg
     1201 tggaagcaca tacattttcc caagtttcag aatattctga aaaaccaaca ttatcagaag
     1261 ggcatccaac tgcacagcga tgactcagga cgggggtgcc ggacaggtcc agaacggcaa
     1321 cggttcattc tgcaggtgct ggccagaagg ggtctgtata gaggtcggcc tcactcgcag
     1381 acgacccgct cctctccggt ctgctcggtc atgagacgct cgaagtactc gtgcgcctcg
     1441 atagcggcct tcgccccctc gctggcagcg atgatgatct gcttggcctt gacgcaggtc
     1501 gcgtccccgg ccgcaaatat cccggggaca ttcgtgtggg tgttctcgtc gatgaggatc
     1561 tcgcgctgct cgttcaacgc caccagatcc ccgaggaacc ctgtattcgg cgcgagcccg
     1621 atcgcgagga agagcccctc cacgtcgagg accgtctcct tcccggtatc ccggtcgcgg
     1681 accgtgatcc cggtcagggc cgcttcaccg tggagttccg tcacctcgtg gtggaggtag
     1741 atccgcacgc cctgttcctc tgcccgggcg gcgtagacct cgtcgcaccg tagagcgctc
     1801 cggacgacca gggtcacgga actcgcgatc ttcgtcatct cgatcgcggt ctggagcgcc
     1861 gcatttccac cgccggcgac ggcgaccggt ctgccgtgga agaccggggc gtcgcaggtc
     1921 gcgcagaccg agatacccct ccccatcaac cgatcctcgc cagggagccc cagggtccgg
     1981 gcctcttttc cgggagcaag gatgactgtc cggcaacgga aggagttctc cgagacagtc
     2041 cggacgacga agacgtcgcc ctctttctgg atgttctgga cgctgtcgag ctcgatggcg
     2101 acttcgagtc cccggacctg ctcctcgaac ttccggataa ggtcctcacc cgtgatcgtc
     2161 gggaacccca tgtagttctc gatattccag ctccaggtcg cctgaccgcc gacgttctcc
     2221 cctataacga gagtcttcat gagtttccgg gcgcagtaga ccgctgctgt cagcccggcc
     2281 ggcccggcac ccacgatgat cgcgtcgtag accccggccg tcaccggcgt cccgaagagt
     2341 tcccggagcc ccttggcatc aaaccccacg atcacctcgt cgccagagac cgtaaccggc
     2401 accccccgct gccccgagac cgctatcatc tcccgggctg cctcgcgatc cttcccgacg
     2461 tcgacgagtt cgtattcgac gttgtgtttc ttgaggaacg cctggaccat ccggcagtag
     2521 gggcagctct ccgtcgtgta gaccttcaca ccagccatga gatggagtat ctccggagcg
     2581 ctatttaact tgtgggggat gaggagagca gaccgggtgc ggaggggttc gtggttagcg
     2641 gcggcagact gaaaaagagg ttattgaatt tcgcggaaca tccgatccgg aaccccgcag
     2701 atggggcact tctccggggc cgctccaagc tcgatgttcc cgcagaccgg acagagatag
     2761 atcttctcga cgtcgaggtc ttttccttcc cggacggcct cgattgcctg gaggtagagg
     2821 ttcgcgtgca cctcttccgc ccgcatggcg tgcgtaaaga cgatcgaagc ctcgttcttc
     2881 cgttccccct tcgcctcggc gacgaacgcg ggatacatct ccgtgaactc gtagttctct
     2941 ccctcgatcg cacccttgag gttctcctcg gtgctcccga cggcgttcat gatgaggagg
     3001 aggcgtttcg cgtggacggc ctccgcttcg ctcgccgccc ggaagagttt ccctactgcc
     3061 gggtaacctt cggcggctgc cttctctgca aagaccgagt acttcctgtt cgcctgggac
     3121 tccccggcgt acgcattctg agcgttttca tcagtcggca tggatggatc attgcgggac
     3181 gctcttatta aatctttcac tttcttcccc cccggaaacc ctatgccgct ggagaaccta
     3241 ccctcggtgg atgcaggttg cgactgccgg aacggcagga gctcgagaag accgaaggtc
     3301 ttcgagtgcg acggttcgta gaaccggagc aggagcacca gaggtgcgac ttgcgacggg
     3361 ccgaagaggt ggagcatgag cgccaaaggt gcaaacggaa ctcgagaaga ccgaaggtct
     3421 ccgagtgcga cgggcggaac ggcaggagca ggagcaccag aggcgcgagt ccgggtgcag
     3481 cgggtcttca tcatcgtcct cgccttaaac ctctccgtcg cactcgcaaa agcggtcttc
     3541 ggcctccttg ccgggtcggt gagcatgata gcggacgccc tccattcggg gttcgattcc
     3601 ttctcgaaca tcgtcggaat agtcgccctc tacttcgccg ggaagccccc cgacccggag
     3661 cacccctacg gccacggcaa gatcgagacg ctcggcaccc tggtcatcgg ggcgatgctc
     3721 ctcctgaccg ccgccgggat tctctacgaa ggctaccggc ggctcgttgc tcccgtcacc
     3781 cccgcgatca ccgcggtcac cgtcggggtc atggtcgtga cgctcatcat caacatcgcc
     3841 gtctccacct acgagcgtcg gcgcggcgaa gagtaccgga gccagatcct tgtcgccgat
     3901 tccctgcaca ccagaagcga cgtctttgta tccatcgctg tcctcggggg ctttctggcc
     3961 gtcaggctcg gctacccgca ggccgacccg atcatcgcct ttgccatcgg cctcctcatc
     4021 acgaggatgg ggatcgggat cctctacgac gcagcacagg tcctcaccga cgcgatgaac
     4081 ctcccctgcg acccggcgct catccgcgcg gtggtgatgg acacgcccgg ggtggccggg
     4141 tatcacgact tccggtgccg gggaaaaatg ggtgaaatct ttgccgatat ccacgtcacc
     4201 gtcgacccgg ccctcccggt ctccctggcc cacgagatct ccgacgaagt ggagcggcgg
     4261 ctcaaagaaa cggtgccgga gctcgccgag gtcgtggtcc atatcgaacc cgacaactca
     4321 ccctaacagt ttcccgagtt cgtagccctt ccggtaggcc gcctccatca cctccggccg
     4381 cgtcgtgata tcctggatgg tgtccatatc gtttctaagc acctcgtccc agtacgggca
     4441 gtcgatgatc tccccaaacg cccgggcggt catctttgcc ccctcaaaga cgttcgggat
     4501 gttcatcccc gcaattgata tgaagagcat cttccgcttt ttccgccgtt ccggggagat
     4561 aaacgaccgg ccccggtgat acttggccat gtaaaagacc tggaaccggt caatgaagga
     4621 cttgagcctt ccggggatgc ccatcgtcat cacgggggtc gcgatgatca ggccgtccat
     4681 ctcgcgaaac ctggcaaaga tcccctccat ctcgtcctgg atcaggcagg tttcgctccc
     4741 cttgcactgg aagaactcca tgcagggcag aacgtccatg tgcgctacct caagcttctc
     4801 gacctcgcac ccggcctcct ctgcaccccg gatggcctcg tcgaggagac gggcggtgtt
     4861 cccgtcgagg agggggcttc cgagcagcgc gataatcttc tttgacatga tgctgggttg
     4921 cgcacgaccc tattaatagg atgcggagac cgggtcggcc ggacgccctg gccggaaggc
     4981 gaactgctaa atatcttgcg aagcaactat cgctctggtg aatttctgtg gccgaaccag
     5041 aagcaaaaaa aacagcgaag gcaggagaga aaccgggtcc cggccggttc atctgcattg
     5101 actgcgggcg ggagctccgg ctcgacagca ccgatgagga cctcgtcagg tgcccgacct
     5161 gcgcctgcga gatgtacaac tgcctcccga tgacccatat ccggcccgac atcaaaacac
     5221 cggaggatgt cctgcacccc ccggagagga agagcaagac agaataaccc gaggtgagag
     5281 aaatggtgaa cgtatcggca aaagggcagg tctaccactg cgagatctgc ggaaacgtgg
     5341 tccaggtcct ggaagccggc ggcggagaac tggtctgctg cggcgaaccg atggtgcttg
     5401 aggagtgatc gagatggaag agaatcagaa gaacctggaa gggaagatcg gaaaagtgcc
     5461 cgagatcttc aaagagttga aagagacgga tcccgacctc tacagaagcg tcatgggcct
     5521 tgaccagatg atctgggccg acggcgccct ctcaagacag acaaagaaga tcattgcgat
     5581 cgcgatcgcc gccgcgctcc gggacgggca tgccgtccgg gcacagatgg ccggtgccgg
     5641 gaacctcggc gtatcgaagg aggagatcga agaagggctc agggtcgcgt tcctgctcgc
     5701 ggggatgccg gcctacgtct acggcaagac cgccctcgag gagtacctgg gcaaccgccc
     5761 gcggaagtaa atcatggaac cgaagagctg ctccagatgc cggtgatcgg tgagaaagca
     5821 cctgagttcg acgcgctgac cacccaccga cccctgaaac tctcggatct ggcggggaag
     5881 tgggtcatcc tcaggctcac gaaggccctc cagacccccg ataagcacgg cgtcgcgacg
     5941 ctggcgaact gggaggctgg ggagaaagtc atcgtcccgg cgccaaagac ccccgaagag
     6001 atcgagaaga ggatgaacga aggctacgag tgcaaagact ggtgcctctg ctgcaagcaa
     6061 ctctaaaccc ggcacttttt ttgcctccgg ccggacaaca ctccttgtat gacccttgac
     6121 gtccttgcgt ttgcgacctc gccccgccgc cacggcaact ctgagaccct cctcgactgg
     6181 gtgctcgcgg cgatggaaga ggaaggggct gcggttgaaa agattatcgt cgccgaggcc
     6241 gacatcaggc cctgccgcgg gtgcaacgtc tgcgaggcgc tcaaccggtg cgtgcaacgg
     6301 gactacatgg actacgtcca cgaccggatc gtcgaggccg actgcatcat cctcgcgtcg
     6361 ccgatctact gcatggggct tgcgtcgcag gcgaaggcgc tggtcgaccg gacccaggtc
     6421 ttccgctccc ggaaatacgt cctccatctc ccgatcgtcc cgccggagcg gaagggaaag
     6481 cggatgggca ttttccttgc gaccgccggg cagaactggg actacgtctt cgatgcggcg
     6541 atcccctcgg tgaagtgctt cttcaacgtg atcgacgtca ggaacaagga tctccggtat
     6601 ctgatggtga acggcgtcga tgagaagggc gcgatcgaac gccacccgac cgcccggcgc
     6661 gacgcgcagg ccctggcgcg ggaagtcatc gaacacctgc gggaggttgg agcggcatga
     6721 ccgaacccgt aagagtcctc ggcatctccg gcagcccccg gcggcacggg aacaccgaga
     6781 ccctgctcga cgccgtgctc gagggcgccc gggaggcggg gggcgtggtg gagaaggtca
     6841 tcctccggcc gcttgactac gcctcgtgcc aggggtgcaa cgtctgtcac cggaccgggg
     6901 tctgcgtcat cagcgacgac ctcacggagg tgttcgagaa gctcgccgcc gctgacgtcc
     6961 ttgttcttgc atcccccatc tactcgatgg ggatcacggc ggaggcgaag gggctgatcg
     7021 accgggccca gtacctctgg gcacgcaagt tcatcctgaa gaatctctac ttcactgccg
     7081 agcatacccg gcgtcataag gggatcttcg tatcgaccgc ggggcttggg tgggagaacg
     7141 tctttgatgc ggcatttcca gcgatcaccg ccttcttcaa caccaccggg tttgagtact
     7201 gggacaacgt catagcaaac gatctcgacc gctacggcgg aattacgggg caccccaccg
     7261 cactcgcaga ggcacgggag aaagggcgga gcgtggtcga cctcctccgg aagccgtaga
     7321 cgccggaaga tgcggaggcc tgatcttccc ccgtccgcgc gcggccgacc ccgggacgca
     7381 gaagtgaaga atattcccca tttgtcgatt ctgctcggga tcagccgcga cgtcaccggt
     7441 cggaagcagg cggaaaggca actccagttc caggcccggg tgctctcgca ggtggacgac
     7501 gcagtgatcg caatcgacac cgggggacgg atcacctaca tgaaccgggc cgcagaacgg
     7561 ctctatgaca cttcctggag cgaggctctc ggtcgactcg actcggagct cttcacctac
     7621 cagtggatca acccggacga cgaacggaac gcctgggacg cccttcggtc ctcagggacc
     7681 tggcgcggtg ttgtcctcca ccggaagaaa ggtggggagg tcatattcgt cgacgagacc
     7741 gtcagcacct taaacgacgc ggacgggagg agcaccagga tgctttgctc cctccgcgat
     7801 gttactgcgc agaggcgagc cgagaaggaa ctccggatca aggatatggc gatagcatcc
     7861 tcaccgagcg cgtttaccat ccccggcctc aatggccgcc tgacctatgt caaccaggcg
     7921 ttcctctcga tgtgggatta tacctctccc tccgaggtgg tcgggaagcc ggtcaaggag
     7981 ttgtggcatg acggaaagga ggccatgact attcttcaga cgctccttga gaccggcaga
     8041 tacaccggtg agcgggtcgg caggaggcgg gacggatcga cgttctatac catgttctcg
     8101 ggaaacgtgg tcacgaacga cgccggggtc ccgatcggtc tcgctgcgtc gctcaccgat
     8161 atcaccgacc agaaacaggc cgaagcggaa gtccaggccc ataaccgagc cctctcggtc
     8221 ttaaaccaga tcatcggcgt atcgacgtcc gcgacggata tcgacgaggt gctggagagc
     8281 gtgctcgcgg ggacccttac tctgctcgac cttgcgggcg gcggcatcta tctgatcgag
     8341 ccgggaaagc agagggcacg gctcgtctgc acacagggcc tcccagaggg gttccccaag
     8401 cagccgtgca tccccgatat caccctcgcg ccctacgcgn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ctcctcgtcg
     8641 atatgctcga cggggagacg aagggctacg cagagaaact caggagcagc atcaggaaga
     8701 gcgtcgagat cttacggaac gtctcgacca tccggcggat ccaccacgaa tcggccgcaa
     8761 tcgtgccggt cgacctcgac cgggtgatcc gggacgagat cgcgatcttc tccgggagcc
     8821 ggatccacta cggcggcacg gacctcacag tcatggccga cgcgctcctg cccgaggtct
     8881 tcacgaacct catcgggaac gccatcaaat tcagtgggca ggcggtcgag gtgaccatcc
     8941 gggtcgagga gcacggcgat gaagttctgg tctcggtcga ggacaccggg ccgggagttc
     9001 ccgacgagat gaaagaggcc gtgttcatgc gctttgggca cagcaggaac cggaagagcg
     9061 gccagggtct cggcctctac atcacccgga tgctcatcga gcgttacggc ggccggatcc
     9121 gggtggatga ccgggtgccg ggccgtcctg aatgcggcgc cgcgttccgg ttcaccctga
     9181 agcgggcctg aaaaaaagag agggcctcag aggagcgacc agaggagaag cccgacggag
     9241 gcggccatga ggacggcaaa gatggcaggg ttccaggcga gaaccttgcg cccgtcgaga
     9301 acgctgatcg ggagcatgtt gaaggcggcg atcatcgcgt tgatccggag cccgacgagg
     9361 cccacgaggc cgatgagacc gccgcc
//
LOCUS       DAJB01000150           25107 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_10044, whole genome shotgun sequence.
ACCESSION   DAJB01000150 DAJB01000000
VERSION     DAJB01000150.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 25107)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25107)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25107
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_10044"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    5183..5229
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5108 a         7560 c         7963 g         4427 t
ORIGIN      
        1 ccggtaatct gcgacaaaca ggtctgaggc tcccagagag ccccggacac tcgcaggaag
       61 ccccctgcga aagcgggggg tagttcactg gttacggata gagaatattg tctgaataca
      121 gagagatccg gccaaacagc acccccgaaa cacattctct gggtaaggga catggcattc
      181 tttgataaat tccggacgaa catcgagggg ttacgacagg cgaaagacta tcccggcctg
      241 atcgcggtcc tggacggtcc agaaccgggt aaccgcgccg atgcagcccg ggccctctcc
      301 gccctcgggg tccccgctat cccggatctc cagaaagcgc tcgagaatgc caatcccgca
      361 tcacgggccc ggatgaccgg ggcccttgct tcagtcggca ctccttccat ccccctgctc
      421 ctcgccctga tcctccgggc aagcccggcc ctgcaggcat ccatcgccag cgcaatcgcg
      481 ggaacgggcg actcgatgat cgaagcactg ctgcccgcac tgcaccacga acagccggcg
      541 atccggcgtg ctacggtgat cgctctccag gggatgggca gaaaggcagt tcagcccctc
      601 tccgaagcgc tccatgacag caaccattcg gtccggcgag aggccgcaaa cgtgctctcc
      661 tcactccggt gggtgcccga cgacacctcc gagaaggtgt cgttttacta cctccgggaa
      721 gactggacgg aactcgccaa actccaggga gcagccgtcc cggtcctcct cacagccctc
      781 ggcagcaagg acccccggac acggtgcgag tcggcacgga cgctcgggaa gatccatgac
      841 tcccgggcta tcccggcgct cgtcagggcg gtgaaggacc cccagatgac tgtgcgcata
      901 cgcgccgtgg aggcgctcgg ggagatgggt gacgaccggg cgaaaccccc gctggccgaa
      961 gccctcaatg acccccacca ccaggtccgg atggaggcgg catgggctct tggccggctg
     1021 ggctgggtgc cgcagagcga cctccagaga gcggaatacc tgattgcgaa agagcagtgg
     1081 agcagccttg tcaggatggg gagggtggcc atcctgccgc tcatccgggc cctggaagtg
     1141 gagtattcag gggtccgtac cggggcgagc gaggcgctgc ggcaactggg gcaacccgca
     1201 ctcagcgctc tttgcgtgga ggccggatcg aagaatccgg cacggcaaca gcgggcacat
     1261 aatgccctca attacatccg gcggcgccag gaagagacaa ccctgagcca gcccgcgcag
     1321 gaagactcct cccattacga gaaggaactc aaagaggggc ttgccatcca gaaacggttc
     1381 gagaagcagt acggacgccc gaactatgcc ccaaaaccct cgccgcagcc cgtgacaccg
     1441 gcgggccccg cacccaacag ccccgaaaaa cccgcagaaa aggcggtaga agagccaaaa
     1501 aaggccgtaa gcctcggaga tctcctcaag gagagccagc aggcggaagc ggcgtgggca
     1561 aaggtcaagg cccggctcag ggaggagccg tcggccccgg tgggttcgat accgctggac
     1621 ctgctcattc cgcttgagtt tgaagaggag atcgccggca ccgacgaagc cgagcaggaa
     1681 cccgttctcc agccggaagc ccccgagccg caggaacttg agatcccggt gatcccccgg
     1741 aaggcgccgg aacccgaacc aaaagagccg gttcccgaga agactccact ggaacggtgc
     1801 cttgaagcgc tcaggagcag cgacgaaagc gtcagggcaa cggcgattgc tgcgctgcag
     1861 ggcatgggca gcgaagcagt cgggtacctc atcgaggcgc tcaaagaccc tcactacggg
     1921 gtccgcatcg cggccgcgga ggggctcggc gagatcaagg accctgttgc cgttgagccg
     1981 ctgatcctgc tcctccgcga cgcaagagaa gacgtgcgga tcgccgctgt ccgtgccctc
     2041 gagcatatcg gcgaccgccg ctcgattcgg ccgctcatac tcctcttccg cgacaggtat
     2101 cacggcgtcc gggtcgccgc agcagacgcc gtcgcgatct tcggccgtga cgcgctcggg
     2161 cagcttgagg aggcgctgga cgatccggtg ccggttgtcc gggtgacagc ggcaagggca
     2221 atagggctca tcggcgcaac cgagtcggtc ccggccctca tcgaccacct gggagacgag
     2281 gcgccggaag tccggtggag cgttgcccgg gccctcgctg agtttggctc cctggcaatc
     2341 gagccgctcg ctctggtgct gaggaagggg aggaaggaga tgcgccttgc ggcgatcgac
     2401 gccctcgggg aggtgccgga taaggaggca ggcgacgtcc tgctctatgc ccttgaggat
     2461 gaggatgaag acgtgagggc aaaagcagcg gccaccctca gaaaacggca ggtgatagac
     2521 gtatggcgga gaacgctcgg cagccaggtc agcgaggaag agcgtacccc caaaaagaag
     2581 agaacagtcc ggtcggagga taagaaggcg tttgagcagt ccggccggca ggagatcgat
     2641 acgcttgtcg gagcgctcaa ggagaaggac tggaatacgc aactcggcgc cgctacacgc
     2701 ctcatcatga tgggacgccc cgccgtggac ggcctcatcc gtgcgctcag ggacgagaac
     2761 ccggaggttc agactgctgc cgccagtatc ctcggggaga tgcgtgagac tgcggtcgcg
     2821 ccgctcatgg acgccctgag cgacaccgac cggttcgtcc ggctcgttgc tgcccgcaac
     2881 ctgggtaaga tcgggaacaa acgggctata gaagccctga tcggctccct gcaccaggaa
     2941 cccgacagcg aggtccgggc ggccgtggcg gaggcgctgg gctacatggg gagcaggcag
     3001 gcgatagaac cgctggccct ggcgctgcag gacagggacg aggcggtcaa gatcgccgcc
     3061 gcccggtcgc tcggctacat cggggacctt cgtgccatcg aaccgttgat cctggcgctc
     3121 aacgatgtgg acgaccgggt caggtatgct gcactcgaga ccttaaagga ccccggggat
     3181 acgatgcggg gccacctgat cgaagcgctc cacgcggggg acataacgtt ccgggcgggg
     3241 gtcgccgaag cgctggaagc gggcggctgg gagccggaga ccggcgagga cctgacacgc
     3301 tacctcatgg cccggggccg gtggtccgag gtggaagcgg tcggcgccga tgcgctgccg
     3361 gtacttgccg gggcgctctc cgactcttcg atagaggtgc gggcaaatgc cgtcagggcc
     3421 atcgaacgga tagggggaga agaaacggtt gccctcctca tcctggcgct ccaggacgac
     3481 gcgctcacgg tcagaaagcg ggccgaatgg gccctgatcg atatgggcga cgccgtgatc
     3541 tccaaactcg acctggcggc cgcagagggg cggcctgagg tttgggaggg gctgcagcgg
     3601 atcatcgccg agatccgcgc gaaggggacg gggacgctct agggtatccg cccggctgcc
     3661 ttccagtagt cgtactcgtg gtgccagcgg tccttgttcg ccgtgaccag ggcccgcatc
     3721 cgctcgagca tcgcggcgtg gagtttctcc tgctccggat aggtctcctt gtttgcgatc
     3781 gccttcgcga gtcttttgcc gagttcgtag gccctcccct ccgcaggcac gatcgtctcc
     3841 ggatctcccc cgaggaccac gccgacaccg cccaccgtgt tcgcgcccag ggtctggagg
     3901 acccggttga ggtacgccac gacctcgtcc gctccggacc cgcccgccgt ggcgacggcg
     3961 cacccgtact tcccgatgaa catctggcag tggatggcgt ccgccatccg gtcgagcagg
     4021 gtctttgtct gggcggtcac cgaatcgatg tagttgggtg acccgagcac aattccgtcg
     4081 cttgaaagca tcttctggta caacccggca aagtcgtctt tcttgacgca ctccccccgc
     4141 tcgcagcaga tcaggcatcc gttgcagtac tcgatctgga ggttgcagag atccacgagt
     4201 tctacatcgg ccccggcact ccctgccccg tcgagaacgg cctcgacaag ccggcgcgtc
     4261 tggctccgcg agccacgggg gcttccgttc actccaagta tcttcatgtt atctctcctc
     4321 gtaacgttct ccatcgatcc gctcacccgc agccttatgg gatgtcgtgc gtgacggcga
     4381 tctcggcgtg gtactcccgg ccgcagtcgc cggggagtgg atcgacggtg aagatcttcg
     4441 tctcgcccgc ctggaaccgg ggagcgaaaa tggactcggt cgcccggatc ataccgctct
     4501 cgtcatcgac gagcatgaaa cggatgacga cgtttctcgc gggtgcgttg ccggtattgg
     4561 tcaccgtccc cgtcacagcc cagtaacagg atgcgtcggt tcggaaatgc ttctccggtg
     4621 cggtgacgct ggccgagagt gacggctgcg gcccggcggg cgagtaggtg gtgtgagcgg
     4681 agactgtcgc ctccggcgtc ggcgcctccg gccccgcgga ggtccccatg cagcctgccg
     4741 cgacggcagc gcagacgagg caggcgagga gcggaataac catcaggctc tttcccgaaa
     4801 gagagacatc ctgcatgatg gttcatcgac cggaggatat ataacggatc cggaagatcc
     4861 gatctccccc tgcccgggtg gcgggcaggg gcaacgattt atctctgccg ggttgaaggg
     4921 gcagagcggg aagaccccct gcgcaagcgg ggggtagttc acatgctccc cggataagga
     4981 gccgggaccg ggaggaatac tggtgagcag agaggccgta gagactatcg ggaccgtgaa
     5041 ggtctgcaac gccgtaacgg gagaggtgga agaggttgag agggtcgtca aatccgacga
     5101 ggagtggcgg cgccagctga cgccggagca gttctccgtt gcccggcatg agggcacgga
     5161 gccggccttc accgggaagt acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnna cccggttctt catgagccgg accgaggtgc tctgcaggcg gtgcgacgcc
     5281 cacctgggcc acgtctttga cgacgggccg ccgccgaccc ataaacgtta ctgcatgaac
     5341 tcggcgtcgc tccgattcgt gcggcagagg gacctgcagg gatgaggtag ggagtcacgg
     5401 cgcccggcca agaacccaga ccgggctatc tttgccgatg ccgacctcga gatacgcacg
     5461 ggaggggtca agatcctccg gcacctcgaa gaagaggaat ccctcgacag actcgtgtcg
     5521 atcgagaaga actgcggagt acgaccctcc ccgattggtt ggcccgggcg cttttaacgg
     5581 acgataggtc tcagcggcgt agtggagggt aaacgcgctc tccgcggggg tccggaaacg
     5641 ggtgttgacg ccttctccct tatggccgag atggctcgcc ttgacagcgg cgaggaggaa
     5701 ggagttcccg ggcgctgccg tgtataaggt cggcttcgtg ccggaagtct ggaatgttcc
     5761 caccgacgtt accgtgctga cgatcagcga ggcagcgttc tccccgcggg catcatcgta
     5821 acggaaccgc tcgccgacct gcagagcgcc gggaaacaga agagcaggct ccccggtgag
     5881 gttcatcggg acggtgggag cgtccgaatt gccggcagga gagcggttgc attcctgcag
     5941 ggcttcagag aggtgttcgg ccccgagagc aagatggtcc agtgcatcgt ccgtcacgct
     6001 ccggttcagg ggggtgctcc cgttgagcag ggtgccggct gcggcgtact cgtcgagcgc
     6061 aacgaaaaac tgcgcgtatg cggatgccat atcaggagag acctcgaggg cggcagcctc
     6121 ggcacggtat ccgtccgcga gggaagacat attggccgcc cggctctgga gccccgcgtc
     6181 gtcctggtca tacagggaac agatctgctc cacggagacc tccaggagcg gtatactggc
     6241 cccggcgatc agcgactcca gcctatcgtc gtcgcttggg ggttctgaga gaagggccgt
     6301 atcgtttccc ggcaccagac cgggggaaga tgcgttcacg ggagcgccgc cctcctcttc
     6361 cggcaaagcg ggagagaacg ttgcccatgc tcccgggaag aggagaatgc cggagagaat
     6421 cactccgata aagccgatta ccagcagtac ccctatgcga gagcccctca ttgtcgtaaa
     6481 cgtatcgcgc acaaaaggat attcgttgcg atagggccgg cagaccggca cggcaccacc
     6541 ccggaatgcc gggatcacag catgccggcg gacggaaccg gctacaatgt cttcccgagt
     6601 gcccagacag gagaaccatc cccgaggctc acctggatag agcattcacc cagagtgagc
     6661 gcctcgggaa catcaaagac gatgtatcct gttttcacct cgtaccggcc cagcgtcgcc
     6721 gcagcatacg gctccccgag cgacgtcccg ggtgccagcg ttatcgggga gtaggatgtc
     6781 ccacgatacc ggagcgtaaa atcgcgaggg gccggggtcc gtatggtata catcctgttc
     6841 tcgcttttat gggccaggtt cgtaactttc acctcaacga gcaggaacat cctcccgggt
     6901 tctgccgcaa ccacttttcc actcccatcg acgaagtaga agagaccggt gctcctcgcc
     6961 gactccagta tcagggagat ctcgtttgca cggctccggt cctcgtagat gtatcgctgc
     7021 aggagcgcca gttcttcccc ggatgcggac ccggcgtata accggggcag ggagaaactg
     7081 gtcgccacga tctcttcggg cgcctccagc accggagact gcaggtatcc aaacgcttca
     7141 ccgagatggt tggacccttg ccggttcccc tcgagggctg tatccacgat cgtcctgtta
     7201 agaggggcat ttccctgcag cgcctcgccg gccgcccgat acgactcgag agcaagagta
     7261 aatgaggtct tcagggtttc ccggtcgcct gagacatcca gcgccgttga atccttcaaa
     7321 agacctattg caaatgcctg gagcgttgcg ccgctctcct ttgccagctg ctcgtcccag
     7381 gcgtagaggg cgtaggtgtt ctgcacggag agcagcatga gcgatacgct gttctcttcg
     7441 accagccgga ccagttcgtc atcgtcgtag tccgggacgg gcaccgtgct gttctcagac
     7501 gtctcgttct cgtccgggag ggtgggagag cctgccgtcc ccggcagggg cccgggggtg
     7561 aggttcaggg gatgaccgct ctggagccgc tccagagccc cgccgaggag acaggagatg
     7621 ttccgggcgg cggccggctg cccgccatcg ggtgtgcctg agccggcggc ctccggtggt
     7681 gccggggagg ggctgaacag gatggccgtg aaggtcagga gggccagtat cattgcaacg
     7741 gcatccggga ggtccacagt ggttgtacat ctccccgttc caggacaaaa accttccccg
     7801 gaggccggcg ctccggaacc tttctaagga gagatcgaga ttccggctgc agttagagga
     7861 ggtttccgga cgccctgccg gaacggcgcg cggatgcgaa gggtatcggg accttcgtca
     7921 ctccccccgc cccaacagcg cgaaccatta tcctccgccg gtaccgacct ctgaggcgtg
     7981 actgcccatt catctgcctg ggacgacgac taccggaggc ggggagatct ctggggcgga
     8041 gcgccggcgc cgctcccgga ccttccggca aatgccgccg tcctggaact cggctgcggg
     8101 aacggcaaga ccctcgcggc ccttcttcgg cggtcggcgc gcgtgaccgc ggtcgacgtc
     8161 tctccccatg ccgtcgctct cgcccggcgg cacccgggga cggcggcggc atgcctcacc
     8221 gttgccgacg cccgctgcct gccgttttgc aaggcatctt tcgatgcggt cttcctggtc
     8281 cacatcaccg gccacctgcc cggcccgggc cggaaggccg tggcatccga ggtctgccgg
     8341 gtgctcaggc ccggcggcgc ggccttcttc cgggggttct ccgtcgagga tatgcgcgcc
     8401 gggaaaggag cggagacgga gcagcagacc ttccagaggg gcgacggcat catcacccac
     8461 tacttcaccg aagacgagac ggcggaactc ttcgcacccc tcgtcccggt ctcggtccgg
     8521 acatgccgct ggcagatgcg ggtgaggggc agggacctcc cccgggccga ggtcgaggcg
     8581 acttttcata agacggtcta gtggcgggaa acgggttttg cgcaccccgc catccccctc
     8641 ccccggcccc tgcgggagcc ggaaccggag acgaacatac cttttttacg cttccggatc
     8701 gagatatagt accaggatac gggaaacccg ggagtgaacg caggcaaaaa cggctcctct
     8761 cccgggcccc cgtgcagtaa ggtcaacctt ttttgacacc cgaacaaaat aacaggagag
     8821 gaagagaggt catgtgcatt gcaatgcctg ctgaggtact ggagattaaa gagggcaaca
     8881 tcggcgtcgt cgacttcgga gacctgcaac aggaggtcag gctcgacctc gtggacgtga
     8941 aagtcgggga gttcgtgctc gtccacgtcg ggttcgccat ccagcgtctc agcagagagg
     9001 aggggcttga gacccgcgag gtcttcaggc aggtctacgc tgcgatgtcg gaggagtgat
     9061 gcacgagtac agcatcgcct acgacatcta cacgaccgcc cgccgggccg cgcttgataa
     9121 cggcgcaaaa gagataacat gcgtctccgt ggacgtcggc aagatggcta tggtgaaccc
     9181 cgagcaggtg gagttcctct tcaagatcat catcgaggac gacccgctct tttcgaacgc
     9241 ccggctctcg tgccgggacg tcgaggcgcg cagccgctgt tcctgcggct atgagggaaa
     9301 cgagcggttc gtatgcccgc agtgcgggaa actcccggag atcgttgcag gaatggagat
     9361 cgcagtcaca aacatcgaga tagaagtgga cgaagaatga aagtcaacct catgcacggt
     9421 gccggcggcg aggtgatggg cgagctcttg cgcgtcctca ccaacctcaa gcacaacaac
     9481 gccgggggga tagggctcga gtcgcttgac gacggcgcgg tcatcccctt gaacggccag
     9541 aacatcgttt ttacgaccga caaccacgta gtctcgccga tcttctttcc cggcggagat
     9601 atcgggcgta tcgcaatctg cgggaccatc aacgaccttg cgatgatggg cgggcggccg
     9661 atcgccctct cgtgcggcat ggtcatcccc gaaggtttcg aggttgccga cctcgagcgg
     9721 atcgtcgcgt cgatggatac ggcactcggc gagtgcgggg caaacctcgt cacgggcgac
     9781 accaaggtcc tcgagcggag cgcgcttgac accatcgtca tcaacaccgc cggcatcggc
     9841 gtcgccgagc gggtggtgcg ggacaacggc cttctggtcg gggacaaaat catcgtcagc
     9901 ggcaccatcg gcgaccatgg catcgccatc atggcccacc gcgaggggtt cgattttggc
     9961 ggccagatcc actccgacgt cgctcccctc tggacgctcg tcgagcgggc gctcgccgcc
    10021 ggggatatcc acgccatgaa agacccgaca aggggcgggt ttgcgaacgc catcaacgag
    10081 atggcgagaa agagcggtgt cggggtcgtc atcgaggaag aggccctccc cttccgggcg
    10141 aacgtccgga gcgccgccgg tatgctcggc ctcgacccgc tcgaggtggc aaacgagggc
    10201 aaagtcatca tgggggtcgc ccccggcgat gttgaggccg tcctcgcggc actccggtcg
    10261 catccctacg gacaggacgc cgcgatcgtc ggcgaagtcg ttgaaggctc caacgtgata
    10321 atgcggaccg ctatcggcgg agagcggttc atcgaaccgc cgatcggcga cccggtcccg
    10381 cgtgtctgct aggagacgac catacctttt tacatctgcc ctcgatcctg ctgatttata
    10441 catcgctgtt atgcagtctt atggagaggc gtgaatgatg gaagaagcag gcatcgaggt
    10501 gcgcctggtg gatgtctggg acgtagagac gatcgccgac ctctaccggg cggggggctg
    10561 gtggaacgag aaatggaacc ctgccagcct cggcggcctc atcgccggaa gtttcgcgtt
    10621 tgccgtcgcc atcgaccctg cgaccggcaa agcggtcggc atgggcaggg tgatctcgga
    10681 cggggtctcg gacggctaca tccaggacct ggtcgtcctc cccgggcacc ggggcagggg
    10741 cgtcggaacg atgatcttat cagcgctgct ggattattgt acctctgcag gtgtcgcctg
    10801 ggtcgccctt gtcgccgaac ccgggactga aacgttctac accgcgcttg ggttccggag
    10861 aatggaggga cacataccca tgcggtggta tccgaaagac tggtgagaca tgctgaaatt
    10921 tgctgacttt aaccccgtga ggttagacga tcgcgacctc tttctggagc actaccggca
    10981 gtacccccag gtgcacagcg acaacacgtt tgcgaacatg atctgctgga accattatgc
    11041 agattaccgg tttgcaaaag tcgaagggtc gatcatcctc tcaagcacca tcaataatgt
    11101 cacgtcgttc cggatgccga tcggccctaa aaacccgggc ctggtcgagg acgtcatcga
    11161 tctcgcggtg agggagggcg gcgacaaacc cctgtgggtc ctcgacccgg taaatgaagc
    11221 gctgatccgg gaactctacc cggcccttcc cctgcacgaa aaccaggact tcttcgacta
    11281 catctaccgg accgaagccc tcgccgacct cgccgggaag ggctatgcca ccatccgacg
    11341 ccaggttaac cggtttgggc gggagtatgc gtatacggtc gaagcaatca cggaagacaa
    11401 catcgacgag gtctgggagt tcctggtcgt ctggtgcgag tggcgggact gcgactccga
    11461 gcccatcctc gcggccgaaa aagaagccat cctcttcgcc gtgacccact tcttcgctat
    11521 cgggcttgag gggtggatag tcaggatcgg cggaaccatc ggcgcgatat cgatcgtcgg
    11581 gcgggtcaac gcggatatgg cggtcgtcca cttcgaaaag gcgctccccg agacctaccc
    11641 gagcatctac aaggtgatca cgaccgagac ggcggcgggg ctccgggacc gctaccgcta
    11701 cgtcaaccgg gagtgcgaca tgggcgtgcc cggtctccgc gaatcaaaga cgcgttacca
    11761 cccggcgtac atggtcgagg tgtactacgc gaaccgggaa gacctggagg cgtgctgcag
    11821 gtgaccgccg atctcgtgct ccggaacgtg gtgctcccga cgggacggcg cgccgacatc
    11881 gtcatcgccg ggggtgtcgt ccggcacgtc ggggcagcgg tgcgtgccga tgagacgatc
    11941 gactgcagca ggctcacctg tcttccggga gcggtcgata tgcacgtcca catgcgggga
    12001 ggcatgcagg cagagaagga ggactggcgg acgggcacca tgagcgcggt cgccggcggg
    12061 gtgacggtgg tcgtcgacca gccaaacacc gtccccccga tcaccacacc cggactcctc
    12121 cgggcccgtg tccgcgaggc ggaggagcat gccgtctgcg ggtttggtgt gaacgcaggc
    12181 gtcgtgccgg gcgccgacct cccgggcatg tacgatgccg gcgcgatggc ctttggcgag
    12241 accttcgcgg caccgtcgag ttacggcgag ggactcgacc cggataccct caaggaactc
    12301 tttgcccgca tccatgccct cggggggctt gccacggtgc acgccgagga ggtcatcggt
    12361 ccggcaccgg caaccctcgc cgaccacgac cgcgcacgct ccggcgcagg ggaggcgcgg
    12421 gccgtgcggg cggtctctgc cctcgccccg gctgaaataa ggctccactt ctgccacctc
    12481 agcaccgccg cctcggtcga ggccgcacgc ggcacggtgg aggcgacgcc gcaccacctc
    12541 ttcctcgcgc gtggcatgtt cgaggaggac gacacccggg cccgggtgaa ccccccgatc
    12601 agggacgagg agacccggcg cgatctgtgg tcctgctggg ataagatcga cgtcgtcgcc
    12661 tcggaccacg ccccgcatac gttccacgag aaggcgctgc cgtttaagac cgctccctcc
    12721 ggtgtccccg gggtcgagac gatggtgccg ctcctgatgg cggcggtgcg ccggaggcgg
    12781 atcacccttg cctcggtgat cgcgaagacg tcgtggaagc ctgccgccat cctcggcatc
    12841 ccgcgcgcag ggttcgagcc gggcgaccgg gcggactatg ccctctaccc ggacgaagtc
    12901 acccgcatcg acgcctcgcg cctccacgca aagtgcggct ggtcgccgtt cgaggggctc
    12961 gatgcagtct tcccaaaaga ggtggtcatc ggggggaagc gggcctacgc ctgcggcgat
    13021 taccgggagg cgtgtgcggc ttggtatccc gggcaaggat atttatcccg ggaaaataaa
    13081 tactacaaag ccgataaagc gcatctttag gtccccgggt gatcatctgg cgagacgcct
    13141 ggatatgatc cgcgggacaa cccaggtgcg cgacaggcac gcctggcata tgggtacgat
    13201 cgggtgagga tcggcagcag ccgcctgtga gcatcgatcg ttagtgccgg gcgacatccc
    13261 tttacgaggc cttttcttat ggaaccctat ctcgacgccg tcgtcgaaac cgcgcacgga
    13321 gtggtgatag cgcttgacgt aaccgcaggc gcaaaacggt ctatctttcc ggcaggctac
    13381 aacgagtggc ggaagagtat tcgatgccag gtgacggccc ctgccgtcgg cgggaaggct
    13441 aaccgtgcca ttatcgacct cctcgcagag acgtttcgca tacctcgcgc cgacgtgagc
    13501 atcatcgccg gacacacgtc atcctcaaag accgtggcga tcgtcgatgt atcgaagtcc
    13561 aatgtggtcg catgcctgaa ggccgccggt gcctgacccg gtacggatga acgaaccagg
    13621 agcaccgtga cgaaacggct ttcatcacgg gagaactata ctgtgagata tattcaaatg
    13681 cgcccttcgt gaggcctgtt tcttggattt ccgctttaaa aaatcttgcg tttaaacccc
    13741 catctgattt aagtaattgc accctagata tataattgac ccctatgccc gcgcgcatga
    13801 atacctgcgt ggcatacggc gatcagattt accttcaagg aggagactcc atgtattcac
    13861 cagaaactac aaagtacctc attcatctta ctctgcagat cgagggggtg gtcgataagc
    13921 ccgacgtagt gggtgccatc ttcggtcaaa ccgagggttt gctcggcgaa gacctcgacc
    13981 tgcgtgattt acagagaaca ggtcgtgtcg gcaggataga cgttcagata accacgaaac
    14041 ggggagagac aaaaggcgaa atactcatct cgtcctcgct cgaccgggcg gagaccgcgc
    14101 tcctggcatc atcgctcgag acgatcgacc gggtcggacc gtgcacggcc cgggtgaagg
    14161 tcgaccgcat cgaggatatc cgggtgacca aacggcgcaa gatcgtcgag cgcgctaaag
    14221 aacttctcct cgaggatttc gatgaaggcg ctataaacag cgacgatctg ctcgacgagg
    14281 tcagggaagt catcaggata gagaaactcg agtacctcgg tgaagagcgg gtgcatgccg
    14341 gtccgaacgt catggcctcc gacgccatca tcatcgtcga aggacgggcc gacgtcatca
    14401 acctgctgcg ctacgggatc aagaacgcgg ttgcagtcga ggggaccaac gtcccgcgca
    14461 tcatcatcga cctctgttcg caaaagaccg cgacgaccct cctcgacggg gaccggggcg
    14521 gggaactgat cctccgcgaa ctcctgcagg tcgccgagat cgacttcgtg gcatacagcc
    14581 cgcggggaaa gagcgtcgag gagatgagcc gcaaggagat cgtcaaggcg ctccggaaca
    14641 aagtgcccgc ggaggtgatc accgaccata cccccgacga ggagggagtg gagcggatcc
    14701 ctgccggtgc atacgcggca acggaagacg agcagccgcc agagcagcag agagagagga
    14761 gccagaaacc gccctcgacg ctcggcgagc acatggccga tgtccggaat agaaatattg
    14821 cacggttcct ctccccggat tataccgtcc tcttagaatc gaacgctacc gatgtcgagg
    14881 gcgcactcca gaacctgaac ggcgatgttg aagggcttgt cgtcgatcgc atcatcgacc
    14941 agaaactcct cgatcagatc gggggaaggg atctcgagtt cgtagctgcc cgggacttta
    15001 aggggattat aaagcgcccc ctgtccatca gactcataaa gatcgggtga aggctatcga
    15061 gggtgcaaat ttatatatat ttacatccac catttttatc tctgggagag aatatgcaca
    15121 aagaagagtt aatagcctta catgggattc tcactgagat taaagacttt tttgaaatgc
    15181 agaacccgga gttgaaattc tcgcagtatt acgcgctgaa gatagaccct tctcaggtgc
    15241 ataagagcaa aatggagcat aaatatgcta tattcgtgct cggaacggaa ctggcgaatg
    15301 ccatgaaaga tgtggagttc tcgtcatcgg gccggatctc agcccggatg aaggaacttg
    15361 cagagaagac cttaaaagag atcgagtatc tcgattgaga acgcccgata tccacgtctc
    15421 acccttcaat acgacaccgg gggcctaagc cccttcgatc ctctcctcag gcacgcatct
    15481 cgcgttcacg ttcgatgagg tacgtagcga gcagcttcgg gatgggtgcc gccatgcagt
    15541 gccagttcgg cgtcccgttc acctcaagga ccgtgtagcc ttcccggtcc cggaggagat
    15601 cgacgccgca gtagtcgatc ccgaccgccg atgccgacgc ctcggtgatg gcgcgcatct
    15661 cctcgtcgat cgggaccggc ataccgatcc cgccctggtg gatgttgtgc atcagcgtat
    15721 cggatacccg ggttatcgcg ccgacggcct ccccgccaag gacgaagaca cgaaagtccc
    15781 ggtcatttgg cacgtactcc tgcaggtacc agggtcccgg cccgaggtcg tcggggtccg
    15841 tcaccagccg gataccgttt ccgtcgtacc cgtagagcgg cttatagacg atctttccgt
    15901 gccgcagaag aaagtcccgt gcctgcgtct ccgatcgcat gtagcccgtc tccggtgtcc
    15961 gcactccatt ccgtagaagg agagcggacg tcatcacttt ggatgcacag gcaacgatgc
    16021 tcgcgggggt attgatcacc cggttatgga gcgagagcac gtttaaggtc tcgaactgat
    16081 gcccgtcctg ccgcatcccg cagacccata cgagttcatt ctcaagcccg gagtcgaggg
    16141 ggtcgattgc accgaggtca aggaccccgt atcgggcatc ggctctctcc agctcccgta
    16201 taacagcgcc ggtcgagttg tcgtccggcg tatcggtcgg ttttggtacg atctggatca
    16261 tacgtctcac atcccgctcc cggggctgac aggaatttca gtaccatttt gtaccttgcc
    16321 ggatataatc ggtagggaag aataccatgc aaaagaaccg ggtctgtgtc atcgggtgcg
    16381 gcaaccccct catgggaaac gatggggcgg ggattctaac gatgcggctc ttcgaaggga
    16441 agttcccgga cgtcgacgtg atcgacggcg gcaccggggg ttttggcctg atccccctga
    16501 tggaaggata cgagaaggtg gtgatcgtcg atgcgatggt gggtatcggc gaccgtatcg
    16561 gcgacgtcct cacgtttgaa acgccgccat cctgggatct cccggcatat gcgctccacg
    16621 atatcgggat tggggaggtg gtaacgatag cccgcgaact cggatatgct tcggatatcg
    16681 tgaccgtcgg aattgaagca ggcgagatcc aggcattcag tcaggagata gacccggcgg
    16741 tcgaagaggg gatccgggtt gccgggcagg tgatcctgag attcttgcag gaatggaccg
    16801 gggagtccgc gagtatcttc tgaaggcgcg tcactggagc atccggatct ctgcgtatcg
    16861 gccttatctt caaagatata aatggcaaga tttatttcat accacagaac taaagttgtc
    16921 tgaatgcaac tgccacgcgg tcggttccat cgccttctca agtccacgac atcccgtgcc
    16981 ttgatagaag agatgggctc aacacagttc accgggatct gcacgatcgt ccttggcagc
    17041 gagagtgcta ccctggtgct gaacgaaggt caggtggtgc ttgccgagca gggggacttg
    17101 aaggggcagc acgtgctgga tgcgatgttg aagggcgaag agaacgaagt ggcagcagag
    17161 ttgaaccttc tgacgcctga acaggtccgc ctcgccctgg agttcaaccg accatgtacg
    17221 accggcgatc ccgcaggaca gggaagaccg tcgccgggta aggccactcc gggagggacg
    17281 aggccgtccg cgacgccgaa gaaggccatt gcggtagcct cgccgaagcg ccggcaagag
    17341 cagcctgccg gggtacacca tattcccatg ccgggagtca aatccgtgaa ggagacggcc
    17401 gaggcgtccc aatcaggaag cgacgagatt gacacgctcg tccagaacat ggaagagatg
    17461 gacgtcgatc aactgatcag cagtttcagg gtaaactgca agaatatgct ccggaggatc
    17521 catctcgatc atctcatcca ggacaaagaa acgtaacaga tgcaggggtg gacaacagaa
    17581 tggactcgac aggcatcatc cagtttttac tcatcatcct cacaatagcg gtggtagccg
    17641 gctttgccag taccgcgtat taccttgcgg agattcgtcg gactctcaac aggcccgctt
    17701 ccccacgggc tccagcaacg ccggaggagc cttcggaccc caccgggtcc atgcccctcc
    17761 ccctcagcga tatcgatcct cggagtacag gaagtctgga ggagtctctc aatgcaatca
    17821 gcgagagata tggtctttcg tccttcacgc tggcgaccgt cgacggcctc ctgatcggct
    17881 cgacgaggcc cggcgccgag gacgaggccg cgcggtacag ttatctctac acgcagggga
    17941 agttgcatga cgaggccgga gttgaactcc tcggagtccc ccaccacggg gagacggtgg
    18001 tcggcatcgc ccacctttct gagcatctct ctgctgaaca gatgagcaca cttgaacagg
    18061 atatccagga caccctgcag cactgggtgt aaggccggaa caatcaggac aacgagtgat
    18121 tacatggtaa aagtatacac gattgcgtcc ggtaaaggcg ggaccggcaa aacaacggtc
    18181 acagcaaatc tggggccgat gcttgctcaa tatggaaaga agacatgtat tctggatgtc
    18241 gacgtcggaa tggcgaatct cgggctcatt ctcgggcttg agaacctgcc ggtgaccctg
    18301 cacgaggtgc tggcaggcaa agcccgcgtc agggatgcca tctacgatgg gccgttcggc
    18361 gtcaaggttg tcccgtgcgg gctctcgtta cagggtttcc agcaatcgaa cccggatcga
    18421 ctcaaggata tcatggcaga cctcgtgagc gagttcgaca tcctgatcct ggatgctcct
    18481 gcggggatca gcaaggacgg cgtcatcccc ctgactgttg ccgacggggt gatccttgtc
    18541 gtaaacccag agatctcctc gatcgtcgac gccttaaaga caaagatcct gaccgagacg
    18601 gtcggcggcc acatcgaagg ggcgatcatc aaccgggttg tcggtagtgg ggacgagttc
    18661 aaccgcgcac agatggagaa actcctcggg gtccgggtgc tcggggtcat accggaagac
    18721 cccaacgtcc ggcgcgcgtc tgccggaagg tcgccgatcg tggtgaagta tccgacatcc
    18781 ggggcatcga aggcatttaa gcgcatctct gccgatgtag ccggcatcga gtatgtcgag
    18841 gaggagacgc ccgaactccg ggagagcttc gtcgatcggc ttgcccgtgc tctcttccgg
    18901 gcaaaggcct gaggattgcc cggccgatcg ctctcattac cgcctttctc tttttggtct
    18961 tcaccacgat tgcagcggca ggcggagatc ggacccgtgc gagcgttggg tcgaactcac
    19021 cggtaccggt catccccggg gttgcaaggg acgcacgatg cgcccggggt aaagaccgtc
    19081 taaaacagcc taccacaccg gccggattct gccgtcaacc cgagggtggg gccatttacc
    19141 cttacgaatc cccaggcccc ccgtatggcc agaaggtacg gtgaaaccgc atagaatccg
    19201 gtattctctc ccgagacgaa gaggcttgca gcgaacaaac tatatatttt taatagcagg
    19261 aagtggtaat cacattcaga gggctcgtaa tggatataca tgtggataag gacgcgtgcg
    19321 tcggatgcgg tctctgcgtc aaagactgcc cgatgcacgt gtacgaactc cagaacaacg
    19381 tgagtgtcgc ggtcaggccg aataactgca tgggatgtct ctcctgccat gagatctgcc
    19441 cggcccaggc actcgagcac cgcggcatct acgctgccaa gaggcattac atcgacatta
    19501 aggtctgtga gatgcttcgg aaggtggtct gatgagcgcg ggatacatcg atgaactgca
    19561 tgaggccttt cactccgatc tcgtctatcg ctccgaggat attcccctgg agtgttcgcc
    19621 gaagccaaaa gagatggaac agacgctcca cggcgtgatg aaactcaacg gtctcgtgat
    19681 caggtcgctt gaggagatcg caggcagggg ggcaaacgca gtcacctacc gggccgggaa
    19741 gaagttcggc cacgaggtcg ccaaatactt ccagaagcgc gaggacgtgg agggggcgct
    19801 tcacgaactc tccgacctgc ttcgcggcca gtactccttc gaggtctgga agcccgagga
    19861 taaggagacg ttcatcatcg aggagaacgg cgagaccttc atctacctcg tcttccacga
    19921 ctgcatcgtc cgccagacgc tccggcgcaa cggtctcgac cagggcggtc cgctctgcca
    19981 gacgctattc ggctacgtcg tcggcgcgat cgaggagatc accggcagga aagccaaact
    20041 ggagatcgtt cacaccggcc cgaacgcctg cttgaagaaa ctgattctga aatgaggcag
    20101 aatgataaag attgctattg aagaactggc agggtgttcg ggatgcacga ttgcggtgct
    20161 cgatctccac gagatgcttc tggatgtcgt cgcggaggcg gatattgtct actctccggt
    20221 gatcatggac gtaaaagagc cccctgaagg catcgatatc gcgttcgtga ccggggcagt
    20281 ccggaatgaa gagaaccgcg aacgcctcga gaagatccgg aagcggtcga agacgctcgt
    20341 ggcgctcggc acctgcgcct gctacggtgg gatatcgggc ctctcgatgc tgagctcgaa
    20401 cgaggacctc ttctcctgcg tctaccggga agtggagacg gcaaagccca acggggttat
    20461 cccgacagac gtgcctccgt tcctctaccg ggtgttcgcg gtgggagacc ttgtaaagat
    20521 cgattactac gtcacgggct gtccgcctaa agaggcgttc ttaaagcaga tcatcccggc
    20581 gatgatcgcg ggcaataccc tcgaactctc acgcaaatcg gtctgtgcag agtgcgacag
    20641 gatcatgggg caggtagaga actggaccct caagcggcgg cacgagggtg ttcccgaccg
    20701 tgagcactgc ctgctcgggc agggatatct ctgtctcggg agcgtcacct tcgggagatg
    20761 cggggcgtcg tgccctaaaa acaacatccc atgccacgga tgcaacggcc cgtcgctcga
    20821 tatcctccgt gagccctgcc gggacatcca cggcatgatg gtgcggagaa tttcggatct
    20881 caccgacaag ccacaaaaag aggtggagaa ggaactctac gacgtcgccc acacgatgta
    20941 cgccttcacc atcgggagcc tgatcatgga ggataaagag acgtcaaaga tccgggacct
    21001 cgttaaggag aggagcaaat gaaggagatc acgatcagcc cggtgacccg gatcgaaggc
    21061 cacgccggcg tcaagatctt cctcaacgac cagggagaag tcgactcggc ccacttccag
    21121 gtcgtggaac tgagaggatt tgagaaattc ctcatcggcg ctgcaatcga ggaagcaccc
    21181 cggatcacgc cccgcatctg cgggatatgc ccctcggcgc accacctggc ggcggcgaag
    21241 gcgaccgacc agatcttcgg tgtcgagccg cccgcgaccg gaaagaaact ccgggaactc
    21301 ctcagcgtcg gccagttcgt ccattcccac gccctccact tcttcatgct cgcggccccc
    21361 gacttcatcc tgggccacga tgcaccggcg gagacgcgca acgtcatcgg gcttgcccgt
    21421 cactcgccgg agctcgcgaa gaaagcgatc gcggtccgga agttcggcca gcgcctgacg
    21481 gaagcggtcg gcggcaaacc catccacccg gcaaatgcgc tccccggagg gatgtcgacg
    21541 ccgctcacgg aaggaaagag agcggaactg gctgttatgg cggctgaagc gctcggaatc
    21601 gcccgcgaag gatgggacct cgcacgcggc ctcctcgacg gcgtcgatac ggagatcggg
    21661 gctgtaaaga caggattcat gggcatgacc aatcacgggc tctactcgac ctgcgatggg
    21721 cctgttgcga tgctcggccc ggacggaggc agagtaggtt cgttctcagg cccggagtac
    21781 acgaacttca tcgaggagta ctcggaagac tggtcctacc tgaagttcgc ccggttcaag
    21841 ggcggagact actaccgggt cgggccgctc gcccggctca acatcgtgga gaggatgggc
    21901 acggagcatg cggacgctgc tctcgcggag taccggcagc ggttcggcac cgtcacccag
    21961 gctgcgctcg cctaccacct ggcacgctac atcgagttcc tcgcctcgtg cgagcgggcg
    22021 gttgcgttgc tctcggaccc cggcatcacc ggttccgata tccgggctcc ggcggggaag
    22081 gttgtaaacc ggcggggcgt cgggatcatc gaggcccccc gggggaccct gatccacgac
    22141 tataccgtca atgaggccgg catcatcgag cggtgcaacc tcatcgtggc gacctgccag
    22201 aacaactacg caattgaccg gggtgtggag gatatggcac gccgggtggt ggagaacgga
    22261 gcgttgactg aaggggcggc aaaccgtatc gagatgatca tcagggcgta tgacccctgc
    22321 atctcgtgcg caacgcacgc gataggaagg atgcccctcc ggattgagtg catcagaaga
    22381 acgtgaggcg atgaaattat gttaaaacag atcctgggag aatttctgaa acttgatggg
    22441 gttaccgccg cagtcgtggc gggacgggat ggctttgtga tcgagagtgt cgttgcgggg
    22501 gacgtagacg tcgaagccct cggtgccatg gcatcgaccg gcatgggtac gtctgaggca
    22561 atgagcaatg aactcgggaa gggagagatg tcccagatgc tcatcgaact ggagaacggg
    22621 cccatcctgc tctctccgct ctcggaagac gagttgatcg ccatcgtggc gaacgcgaat
    22681 gtcaacgtcg ggcgaatccg gtacgaactg aagaagaaca gggatcgaat aactgctgct
    22741 ctgtgagacg atgctacccg acggcgtctc actggggcga ctgcaggggt ctatcgcggc
    22801 ccttgcttcg atcagccctc acttcacagg agcggtgcgt atcaatgggc ccaaaggggc
    22861 cggttttatc ctggtccaga acgggaatcc atacgcagcc gcgttcgagg acaccgacaa
    22921 aaacctgctg ctcagtggag acgaagctta caggcacctg cttgaccggc cctcgctcaa
    22981 ttatgaactt atcgcgtaca ccgccgaaga gaccgaggcg gcccgggagg cctcccgtga
    23041 gacgggatgc ctggtgagcg gagacggtgc aaagcctgct gcccggaccc ggctggcgga
    23101 gacgagttgc ctcgaccaga tcgcacttca gccgggtgtc attgccgtct cggtctttca
    23161 cgaggggttt ggccttcagt cagtgggctc cgcggacttt gaccaggtgg ctgcggtcgc
    23221 cgaagacctg ctccgcgccg gcacccgtat caccgccgat atggagatgg gggatctctc
    23281 ccagatgatc ctcgagacac cgggagggaa actcatcatc gcgccttacg gtgacctctc
    23341 cctctgcata ttcgcgaagc ccgacgccaa tctcggtctg atccggctct cgatacgggg
    23401 catgcagatc gggccggcct gagagaaggg gcggcctcaa caccccccca ctcccggcga
    23461 accggttgtg cagagtgtgc gagtccggtt tacggcaggc tccgaacgta ttttttcggc
    23521 tttgtgaaaa cttttttttc tagccttcct ccctaccccg atgaggttgg tgctaggatc
    23581 ggcttgtccc tggaatcagc ctttttcttc gagcatgttc ccatgcagtg gaccgtggtg
    23641 gctatcgcca ttgggggcgg ggctgacgnn catctcatca gccccacccc acccgcgctt
    23701 cccgggcagg cgtttcccac ccgggctctt tcgcacccaa cagttgatat tccggcgggg
    23761 tagagatagt tctatggatt tccacaccgc acagggggca tggccgacac tccttcttcc
    23821 aggactcccg gccaggtcgc cccgccgtca gggagtgatc gcctgatgaa gtattacccg
    23881 gttcattccg gatacccgtc gtatgccgag gtgaattcag cggttgaggc ggcttttgcc
    23941 gagcacggca gagagggcgt tcagatgccg ccgaaggtct acgtgacgtt cgtcgggaac
    24001 ggggatttcc ggacgatgcc cgcatacctc ccggcgctcg ggattgccgg ggtaaaggtc
    24061 gtcaacgtcc acccgcacaa ccgcgcaaga ggcctgccca ccgtcatggc gctcacgatc
    24121 atcatcgatg tggagacggg tgcgccccgg gcgatcatca acgccaccga acttaccgcg
    24181 atgcgtaccg gagcagcggg tgccgtggca gcgaggtacc tcgcgccccg gcagtccgtc
    24241 accctcggag tcgtaggggc cgggcggcag gcggaggcgc tggtcgaggc gatggcagcc
    24301 gcgctcacga tcgaggaggt ttttgtctgg agcaggacgg agaagagtgc ggaggctttt
    24361 gcagcgcgtt actcggacta caacgcccgg agtgtcccga ttgagcgcgc ctgcgactgc
    24421 gatgtgctga ccacaatgac accgtcgaca gagccgctcg tgatggcaga ctgggtccac
    24481 gaagggacgc acatcaatgc gatcggtgcg gacgcccccg gcaaacagga actggacccc
    24541 ctgctcctgc tgaaggctga ggtcttcgtc gacgacccca gccaggctat ccactcgggc
    24601 gagattaatg tcccggtatc caccggccac ttcgatcccg gccggatcgc gggaaccctc
    24661 ggcgaggtcg tcatcgggaa gaagagacgg tcgtcccccg acgccatcac ggtctttgac
    24721 tccacgggac ttgcgataca ggatcttgcc atcgctgctc tcgtcctcgg ggacgacgag
    24781 ggatacgaac tgccgttccc gtaacatcga ccctgcggag cgtgagcact gcccaggtgt
    24841 gggtgccgga aatgccacaa ccgaaataag gacagacccc atgcagtgga ccgtgggact
    24901 atcgccacgc actgcccccg cccccggggc gggggaggaa gccggaggcc gggcggggtg
    24961 ggggttacag atgtccgcgt acaaggtaga gacaggggag ggggctgctc cccctccccg
    25021 tcagcccctc cccaagggcg atatccccac ggaatccatg ccaagcaatt ccagatgtca
    25081 cgtgtcaact acccccggtt gaaaccg
//
LOCUS       DAJB01000151            6450 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_128847, whole genome shotgun sequence.
ACCESSION   DAJB01000151 DAJB01000000
VERSION     DAJB01000151.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_128847"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1198 a         1998 c         2089 g         1165 t
ORIGIN      
        1 ccggtgagca tcgccgccct cctcgggacg ctcgtgctcc tcttcacctt caagggcgac
       61 gtcatcctcg caaatccgct cacgatcctc tggatcgcga tcccactctt cattcagacg
      121 gtcttcatct tcgcgctcgg ctacttcgtc cttgcccggc ggctgaaact cgcctaccgc
      181 gatgcggcgc ccgccgggat gatcggggcc tcgaaccact tcgaggtcgc catcgcgacc
      241 gcgacgatcc tcttcggcct cgggtcgggg gcggcgctgg cgacggtcgt cggggtgctg
      301 atcgaggtgc cggtgatgct gatgctcgtg cggatctgcc tggcgacaca ggggatgttt
      361 gggggtgagg cagaatgacg aacagcagag aaccccgaaa ggtgctcgtg ccgctcgacg
      421 tgacggagag cggcgagacg attctctcgg cggtcgagga gatcgtgcgt ctcggcgtcc
      481 aggagatccg gctcctccac gtagcgaacg tgcgcaatac cctcgcggct ccggagatcc
      541 tcgaccacga ccgggaggtg ctcgaggcat ggcgggagcg gctggtcgcg tgcggggcgg
      601 cctctgtgac tgtggaggcg gtcagcggca tccccgcgat cgagatcgtc gagcgctcgg
      661 agcgggacga ctacgcgctc attgtcctcg ggtcgcacgg gcggaacctg gtatcgaggt
      721 gctcctcggc agcaccacgg aaggcgtcct tcggaacgct gacgacccgg tcctcctcgt
      781 ccggctgcgg gttattgaag caggcgatga agcgacctgc cgactcgcgg ccgaccggct
      841 ctttgggcgc atcctgtatg cgacggactt ctcggcgtat gccgagcggt gcatcccctg
      901 cctgtcatgg atggccgggg cgcaccccga ggagatgatc gtcgtccaca tccaggacct
      961 ccggcacctt gcgtatgcct cagaagagca gatggaagag ttcaaccggc gcgacgaagg
     1021 gcggctcgcg gcactgaagc ggcagttcga ggcagcgggg tctgcaaacg tcggcaccgt
     1081 cctccggaca gggaacgcga tcgacgaggt gctctccctt gcgaaagaac gcgacgcaac
     1141 gctcatcgtc ctaggagcga aggggcggca cgggattgca gaacagatct tcggcggagt
     1201 ggccgaagtg gtcgtgcacc ggtcggcggg cacgtgctgg tggtacgata attcaggaga
     1261 gatagatatg gcagaacagg aactcgtcgt cgaatggcgg catatcggaa gagacatcga
     1321 gaacacctgc gagcggtgcg gcgagaccgg acgggcggtg atggacgtcg tcgagcagat
     1381 ccgccccatc ctcgaggagg agggcatcgc ggtccgggtt gtcgagacag ccctcgagaa
     1441 cgaggcgatc ggggagtcga acagcatcct cttcaacggc gtgccgctcg aggaccttat
     1501 cgagggtatg gaagttacca gcacgccctg cgcctcctgc gcctgcatca ccgggcagga
     1561 cgatgcagcg tgccgtgcta tcgagtataa cggcgagcgc tacgagtcga tcccgccgga
     1621 gctgatcgcc cgggcggcac tcaaaacgct cgggatagcg actacagtag aataacgtga
     1681 tgagagtgac agcaatcaga gacattcaag cgatgggatc gacgatgaag gagatcctcg
     1741 ggctctccgg gtcgccggtc ggtgtgcgga tcgtgaagcg gggcgatgag attgcagagg
     1801 ccgaacccgc aggagggcac cgcttctgcc aggcgctgat gcgggggagg cgcggcgacc
     1861 acgtcgtcgt gaccgccgag acgatcgcgt gcccggcggc cgcccgtgcc ttcgggttca
     1921 agccgctccc tgaggcgctg aagtcgggga agggtctggt cggtttcggg atcactgcac
     1981 acgaatcggt cgggcagcag atgtttgtcg ggatgacgac ctttgagccg ggcgagatcg
     2041 gccagctcga cgtcttcccg ctggatgccg ccggggagcg aacccgacat cgtgatcgtg
     2101 gaggacgagg tcgagaaact gatgtggatc gtcctcgcct acatgcacgc ccggggcggg
     2161 gaacgggtct ccggttcgac cgccgtcctg caggcgacct gcgtggatgc gacgaccatc
     2221 ccctacgtcg aggaccggct caactacggg ttcggctgct atgggtgccg ggacgcgact
     2281 gacatggcgg caggggaggc gatcctcggg cttccggcgc attatctccc cgagatcgtc
     2341 cgacacctcg agtacctgaa caaaaaggca ctcccgcact cccggggcaa acatgccctt
     2401 gccgcgctca cgaaacagcg gagtgccagc gagcagggtt cctgctcgtc gctctgaaga
     2461 ggggtctgat accatgaaga agaaagtcct cttcgtctgt acccacaacg ccgcccgctc
     2521 gcagatggcg gaaggctacc tgaacgcccg ctacggcgac cgctatgagg ccttctccgc
     2581 cggcaccgca ccgggagccc tgaaccccta cgccgtccgg gcgatggccg agatcgggat
     2641 cgacatctcc ggccaccgct caaaggatct cggtgagttc gacgagcagg agatggacgt
     2701 cctcgtcgcc gtctgcgagg gagggatctg ccccctcttc ccctgggcga aggaggagat
     2761 ccaccgggag tttccggacc cggcgaacct cgccggcacc gacgaggaga tcatggccgg
     2821 catccggcgg atccgggacg agattacggg ttggcttgac gcgacgttcg gggcgatctg
     2881 aatggcgttc accatcgttg cctgttcggg gatctcgaac accgggaagc tgaccgccca
     2941 ggtgggggcg atgctcctcc actgctcctg cggggcggtc gaggcctgca tcgcggccac
     3001 ccggccgacg gcgtcgctgg aggctgcggt ccggcacgcg gatcgcatcc tggtcctgga
     3061 cgggtgtagc gactgctgcg ggagaaagaa ggtgcgggcg ctcggggtcg agccgcacct
     3121 gcaccttgtt gctaccgacc gcgggattgt gaagaatggc atggccgagc cccggttcga
     3181 cgagatcgag cgcctttcag ccgccgtgct ggaggcgatc cggcggtgaa ctgcgggttt
     3241 cccggcaccc ggcctgtccg ggcgaaaggt cggctccggc cgttaccgga gcgggtaacg
     3301 aggccgcaca cgacgggccg caccgcatcc cgctgcgatg cccactgcag gagcggcagc
     3361 attgcttcac ggagccccgc accgtcctcg gtgagcgaat actccacccg gggcgggatc
     3421 tcggcgtagc agtgccgctc gacgagatgc tcactcacga ggactttgag cgtatcggtg
     3481 agggtcttgg ggctgatgcc cgcgagcgca ttctccagct cggtgaaccg gatcgtcccc
     3541 gctctcccga tggtcgtgat gatcaggaga gtccatttct tcgcgatgac ggggagtatc
     3601 ccccggagag ggcaggtgcc gaacgcttca ctatcgttgc acatagttgg tatctgtttc
     3661 gacagtatgc atccttaata ctatactttc taaagtattg tcgggagcac tacaaacgtg
     3721 tatctcgtac gtggatggcg ctgatatgcg ggaacgggag aacactatga ttggaaatgc
     3781 cggagaacac tgttgcggta ggctgaacct catagaaatg gcagaattct cgaaagaagg
     3841 accgatgaag aaagacctga tacgatcggg gcagacagat ctggtcctcg tctgctttga
     3901 gagcggccag gagatcccgc cccatagaga accgtacgcg gtacagttcc tcgtcgtttc
     3961 gggagaaggc gtcatcgttt cgggagacga cgagtatccg gtgaaggccg ggcacctcgt
     4021 cgcggtggct gcgaacgggg tgcggggcat caggtgtcgc acacggatga ttatcctcgg
     4081 gatccgtcag gccggcagag ggggagaggc atgccgggag tgatcgaggc cgcaggcctg
     4141 acgaaggcct tcggcgccat catcgccgtg gaccacgtct cgttcgaggt ccgtcagggt
     4201 gagatcttcg gttttctcgg tccgaacggg gcagggaaga ccaccaccac aaggatgctc
     4261 accggcgtcg tcccgatgga cggcgggagc gtcagggtct tcgggcacga catccgaaaa
     4321 gagcccatcc gggcgaagca gcggttcggc gtcgtgcccg agacctcgaa cgcctacgtc
     4381 gacctgaccg catggcagaa cctcatgcta atgggggacc tctacgggct cgatcgcacc
     4441 cgggcagaac ggcgttcacg cgacctcctc gagaccctcg gtctctccga gaggagagac
     4501 cagaaggtgc aggggtactc caaaggcatg aaacagcggc tcatcctggc gatggcgctc
     4561 ctccacgagc ccgagctcct ctttctcgac gaaccgacga gcgggcttga cgtacagagc
     4621 acgcagatga tcctctccct gttgcgtgcg ctgaatgttg acgggacgac catctttctc
     4681 accacccaca acatggagga ggcgaaccgg ctctgcgacc gcataggcat catccagagc
     4741 gggaagatcg tcgcgatcga tgcgcccgag aaactgaaga ccaccatcga ccgcacgcac
     4801 cggatcgagg tgagctttga ccgggcagtg cccgcccagg ctcttgcgga actcgatggt
     4861 gtgaccggag cgaaccggac gggggataag tggcagatcg ttgcggaaaa cccggatctg
     4921 gccatccggt cgctcgtggc cttcagcgga gaccacggga tggcgatcct caccttaaat
     4981 accctcgccc cgtcgcttga cgaggctttc ctgcggctga cacaggagga gcgatcatga
     5041 acccggcagc gttccggcaa cagatccggc gggcatgggc gatcgcgaag aaggatatcc
     5101 ggatctatta ctccaagggc ccggtgctca tcttcgggat cttcatgccg gtcttcctct
     5161 tcctggcttt cttcctgggc ggccggcagc tccccctcgc gttcctcctc tccgggctca
     5221 tggggatgac cctcttcttc acctcgaccg ccgtatctcc tgcaattttt ccctgggaag
     5281 ggcaggcaaa gaccctcgaa cgccttgccg cctgccccgt caccatcgag gcgatcgtcc
     5341 tcggggacct gattgcctcc atcctcttcg gcatcggggt cacggcggtc acgctcctca
     5401 tcgcccttgc tctcggcctg ccgctcctgc acggtgtcgt gcttggttcc gccgtcctcc
     5461 ttgcggcggt ctgcttctcg gctatcggga tgctccttgc cgtccccccg acaaacgtgc
     5521 cctcgaacat catgatgctc tcctcgctcc tgaagttccc gctcgtcttc atctccggca
     5581 tcttcatccc gctcgagcag atgcccgcat gggggctcta cctcgccgtc tgctcgccgc
     5641 tcacctactt cacggatctc gcccgctact cgtttacggg cgcacattac ttcccggtgg
     5701 ccgtcgacat cgcagcgctt gccctcttca ccctggtctt caccgccgcc gcgatgcgcc
     5761 tgcacaggcg gaccatgccg cggaggatgt gatgtcgtgc cgggaagccg attccgggat
     5821 tggctgcggc tccccacatc gtccttgcag ccggccgctt ctcccgggtg ctgcacctgg
     5881 tgcccctcct cggagaagag gaggaggtgc tccgggcgct cgaacggagc atcacgcgaa
     5941 cctgccggaa ggtgaatgcg atggacagag tgctgatccc cgcatcatcg acactctgcg
     6001 gtatatcaaa gagcagcttg aggagcagga gcgggaggat ctctaccgca ggaagatcac
     6061 ccgggcaaag aagcggggtg agccagagtg accgcttctg cggatcgccc ccgacacgtg
     6121 gtggagagac gtcgatatct tcggcagggg aatgtggtga tgatgcacct ctggagaaag
     6181 catatatatc tgcctgcaca agaagtcaga atcatttcaa accaaattgg aacagtctat
     6241 ccagcgacag gagtgtccta gatgaaaaca aaacgtaaca ggtcaggtat acctgtaagc
     6301 cgcagttggt atgaagggtt ctccctcgaa tgctctccgg atggccgtga ttaccgaccc
     6361 tccattcttc ctcaccgctg agatataccc ccgaaccctg cagaagttag atgccccctc
     6421 cacacaactg agacgtccct gagatcttct
//
LOCUS       DAJB01000152            4478 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_40745, whole genome shotgun sequence.
ACCESSION   DAJB01000152 DAJB01000000
VERSION     DAJB01000152.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_40745"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          846 a         1312 c         1274 g         1046 t
ORIGIN      
        1 ggcaccctga tcaacgccga tgtcaacggc gctctaaacc atttacgaaa ggtagccggt
       61 gataccgtga tcccacggat aatcggtaga ggccgcgtca accggcccgt gcgaataagg
      121 acgtcttttg aaccgtcaac cttcgcaccg attaaattgc agccctgtgc cccacaaggg
      181 cccccgctgc aagcccccgg ttttaaccgg gggtagttga cagcgttcat gtcggggaac
      241 cttcctggtg cagaatctcg cccgcgggcg aaggcttaac tcgcctccct tttagggacg
      301 acagatcccc ggcctgtgcg cggatcctcg agatgtcact tttagcaccc tctgggattc
      361 aacctgtaga gtgaccgata gagaggttca gaccgggcgc gttcctcaag ttaaaatcct
      421 caaattcctc tgttgactat ggaaaaacca tcgttaaata gtagctgcac gataccttat
      481 tagtcaaatt tcgggtattt ccgccttgag gctgcccgaa aatcagcaaa cgatctgcag
      541 atcggggtga gggatcaggt ctccacaccc gggggcgtag agaatgaaga ggtctcaatg
      601 gaagtggctg ctcgtctcga tcagtttcag cactcttgtc atggcgggcg ttctgtactt
      661 caccgtcgat gagacgacga tcgagtatct cagccgtata aacccgtttt acctggtcct
      721 tgccgtcttc ctccacatcg tctcgctcgg gttctgggcg ctccgcatcc agaagatgtc
      781 ggcgtcgctc gggtatcggg tacatttcag gcactgcctg aacctggtgc tggcgaacat
      841 gctcgttgcg gcggtcaccc cgtcacaggc gggcggcgag ccggtccggg ttcatgagct
      901 ctaccgggcg ggcgttccgg tcggcgacgc caccgccgtc gtcattatgg agcggatcct
      961 cgacggtatc gttctcgggg gtctcggagc cttcgccatg ctcttcctcg gcagttactg
     1021 gagcagtctc acctcggggt tcagcggcgt gagcatcatg atgtacgccg cctggatctc
     1081 cgtcaccctc ttcgtgctgg tcttcatcta ctcggtgaag aatccggacc atctcaaacg
     1141 ggccctcaaa tggatgtcgc gatgggtcga ccggcgctgg cacttaaaac gcctggagag
     1201 tctgcttgaa acgatcgacc gggaagtgga caacttcaac aaaggtctcg tcaagttcgt
     1261 gaaccacggc aaaagcggtc tcgtatgggg gatgttcttc acgctgctct tctggttctc
     1321 cgagttcacc atcgcatcgc tgatccttat gggtctcggg cagccgccgt tcctcatcga
     1381 gtcgttcgtc gtccagctcg tcattgcaat catcatgatg atcccgctca cgcccggcgg
     1441 ctcgggggtt gcggagctcg gcgccacgtc gctctacagc ctcttcgtcc cgtcgtcgat
     1501 cgtgggcgtc ttcgtcatcc tctggcggct gatcctctac tatctcaaca tcctccttgg
     1561 cctgctctcg ggtctcgtca ttgtgcggcg cgagtttctc gcccgcgcca aaaagcagcg
     1621 gtgattgacc ccaagattta aacacgataa ggctgatgtc aggtgatcac atgactgctc
     1681 aaagttgccg ggtgcagggc gtgttcatgt cggtgagcga gatgggagcg gcgccgactg
     1741 tcgtcctgga tgccgggggt gacagcacca tacccatcta tgtcgggctc tgggaagcga
     1801 tctcgatcag caacgcattg aacagcgaga tgcttccccg gcctatcacc cacgacctca
     1861 tcgttgagtt gttccggaac tttgagatca ctctcgatgc tctgcacatc gattctctgg
     1921 aggagggggt cttctacgcc aaactgcttc tcaggcaggg gtcgcgcacc gagaccatgg
     1981 actgccgccc aagcgacggg atagccatcg ctctgcgcta ccgtgctccc attatgatcg
     2041 aagatacggt cgttgagtcc gcagccgtaa agaaggatga tctcccgagg atggtggacc
     2101 tcaaagagta cctttgagcg gcccttatgg gacggacctc ttaccggggc gggacggccc
     2161 cggaaaattc taaaatgggg caggccgtta cttccggcgt gcggcgagtt cgtcgagcac
     2221 gtccgccaca tcggtgcctg atgcttcaag cgccaccagg aagtggaaga ggaggtcggc
     2281 cgcttcggag accgtcctct cggggacctg attcttggcg gccaggatga actcgaccgc
     2341 ctcctcaccg accttttcga gggatttgtc gatccctttt ttgtgcgtca ggacggagct
     2401 gacataactc tcggccgatg ggtgctctgc ccggttctgg atgacctgcc agagttcgtc
     2461 gaggacgttc cagtcacgca tgagtctcct cccccgcaag cacctcacca aggtcggtat
     2521 cgaagtaccg ggagaggaga agccgcgcct tctcgatggt gcacccgcct ttgcctatgg
     2581 cgatgccgag atcgttttta ttgatgtata ccatgagcct cccgtcgttt ccttttctgg
     2641 caccgaagac ctcggccggt ttaaacacat ttctcgtaaa tttctcgatc tcgggagaat
     2701 attcgaccat ttcgatacgt ttgccgagaa cccgctgcat cttccggata ttgcttccat
     2761 tccggccgat agcaaggccc atatctcctt cttttatgag gtatactatg cgatcgaagc
     2821 gctcgtcgat gatgcagtcg agcgcggtcg cccgggtgag aattctcaac tcttcgatat
     2881 atcgtcgctc tttaaagcat atggttcgta tcattagaga ggtccccaat gggaatgatg
     2941 cggtatgata tagtcgctgg aagaatatat atctgagtac ccttcccggg agagaaacct
     3001 agcacttttg caggatcacc tttattgtcg ccccctcctc cgatttgccg ggtacccggt
     3061 ccgccgccca gacgcgtccg ccgtactggt ttacgagggt cttgacgata tgaagcccaa
     3121 gacccctgct cccggatgcg gttttgctgc cctcgtggct gaaacggtcg aagatgttgg
     3181 gttttaagtg atccggtatg ccgatgccgg tatcggcgac ggagagcgtc acgatctctc
     3241 cggtctccat gacggatatc tcgatccgga ccttcatgcc gccgaacttg atgctgttgc
     3301 tgatcaggtt ggatacgatg tgttcgagga gggggtttgc ccagaccatg tggctttccg
     3361 gctcgtacct gatatcgatg cctgcgtacc gctgtatctg gccgcgaatg ataccgtcca
     3421 ggtcgaccgg tccgagacgg acacggtgtt tgttgagggt atttaaaagt tcaacattct
     3481 tgatgacgtt gatcccctgc tcgaccgtgt tcttgacccg ttgagcgagc tccgcctcct
     3541 caccggagag ccgttcccgg agcatctcga tgatcgttgc cgcaaccatg ctggtgttgt
     3601 agatgtcggt gccgagcagg tcgaggtaga ggctggagag ctggctggtc cgttcacaga
     3661 ggaggtcgcg ggtgatatcg cggctgatcc cggtggcggc cacgatcttc ccggcggggt
     3721 tgtgcagggg gtgaatggtg acctgaaacg accgctggtc gttgcgccac aaaaacgacc
     3781 gggtctccgc taccacctct cccgctccga tgacccggtg cgcagccccg accagaaagt
     3841 ctgcctcctc cgcaggaaac agggcatggg gtgtcttccc tacgacccct tcggggtcta
     3901 cgcccaggct ccgggcaccg gtccagtaga actgggtgaa gatgccgtcc tggttgagcg
     3961 tgaagatcat atcggggaga gaactcgcca ggatctgcac ctgctcctcc ggtgccgcac
     4021 gagcctcttc gaccgttctc tcttccggag catcccggat ggtctcaacc gcaccgatct
     4081 gcctgccggt ggtgtcgtag aggggggcag ccatacagac gacctctctc ccggatgccg
     4141 tgggaatgcg cgactcggcg agcaactctt ccccttcatg aggaagcacg cggtaacggt
     4201 cgctcccggc gtcgccgtga gtcagaatgt agttcgcgag tgtggggcac gtctcgccga
     4261 acaacgcttt tccatggacg tgaccacctt tccctatgat ctcttctgcc gaaatgccgg
     4321 tataccgttc catgctttcg ttccagacga ccacccttcc ttcgcagtcg agaacgagtg
     4381 ccggttgctg gagatggtcg aagatactca cgggtatctg gctctgtttc atagactccc
     4441 tgactctttc ctgcttcata ataatcaggc aggagacc
//
LOCUS       DAJB01000153            6315 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_539, whole genome shotgun sequence.
ACCESSION   DAJB01000153 DAJB01000000
VERSION     DAJB01000153.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_539"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1318 a         1968 c         1909 g         1120 t
ORIGIN      
        1 ggctcgaaga acagggcgtc gacgtaaccg cgatcagggc cgccgtcgag cagggggatt
       61 acgagaccgc acggacgctc atgaagcagg ttctggagag ccgcaacggc gcactccccg
      121 agaaatcgca gaatggctgc tctgagggca aaggcctctt cgggtgccgg ggcgtcagaa
      181 ctgccgagta aagaggagga ctatcggaca gcagtagggg gacctctcac gcaaaaaccg
      241 ggtcgacgac acgtctcgcc gaaagatccg actccccggc gacaccctgc tgcccgggca
      301 tcgtctcctc tttttcggaa cgcgacgcag gcggggatcg cctgcacgcc gtccgggccg
      361 cagaccctgc atgcgatcct ccttgcagga gctgtcaaga aggctccgca ggctcccgcg
      421 tgccccgctt ccttcgtgtc gtgaggcccg gacggcattc catccgaacg acccctccgg
      481 tggtccgcac ccccgctgcc gggaggaggg ggttcacccg caaagatcga ccttttccga
      541 agcggccggt cgacccaaca tcgttcgggc gagagcccgc ccccatgtcg ttcgccgggg
      601 ttacacatta ataactaaat gttcaagatt gtatacaacg gctgcaatat gaagcgaaat
      661 acattctatc ttctggccgg catcgttgcg ctcgccgagg tcggcatctt ctggctctcg
      721 gtggagctta aaaggccgat cctgatccag gtcgcgttcg tcctcggcgt cctcctgatc
      781 tacctggcac ggcggagagt cgaagaaagg atagaggatg aacggacggc gatgatcacc
      841 cagaaggcag cgctccggac gctggaggta ttctgggtag tcttcttcgt ggtcagcctc
      901 gggagtgcgg tcgttgcgtt ttcgaggccg ctcggactgc gcccgcccca tccgggtccc
      961 cccaacatgg cccctgtcga cgtgctcgaa ctcccgttcg tcggcggttt tgctctcttc
     1021 cagatggcgc tcctctgcct gatgatcttc ctgtacgtgg ggttcaggat gtactacgcc
     1081 cgcaagtatg gagaatggga tagagatgaa gaacaggatt aaggtctacc gggcgatgca
     1141 cgacctgacc caggaagggc tcgcaaacga gctcggggtc acccggcaga ccatcctcgc
     1201 catcgagaag gggaagtacg acccttcgct cgacctcgcc ttcaagattg cccggttctt
     1261 cggcgtcgcc atcgaggaga tcttcctcta cggcgatggg acagagcaga aataatctct
     1321 ataggatcct cttttcgtag aacccccgcc cgccccggtt cccggacagg gcaggtagcc
     1381 acccggcggt cacgacgtgc ctccgacgtc atacaaccac gcgctcagtt tcatgcacgg
     1441 caggcacgcc caacccgggg gatcggatcg ccggtaagcg gatctgttgt cgggagagtg
     1501 tcaggccaac cactcattat gttgcttttt ctgacaattc gtaacatata aataacaata
     1561 tgtaaaaact agtttacatt gtcgggcaac ccatcccgca gatgccgggg gggacgcgac
     1621 accagatcag cagatccaga gcgagtgaaa agaccatgaa aacttccagt aaactaccag
     1681 agcagcaccg gtgccgtgca gagatcgcca tgcacaggag gcagagccga tgaagtataa
     1741 gttgtgcatc ctgctcctcc tcctgtcggc aatcgtgggc gccgccgcgg cggccgatac
     1801 aacgaccgag acgacatcgg cggactcaca gattgcagtc accgggatca ccgttaatcc
     1861 agaggcgctc atgcgcgggg ataccggcac ggtgacggtc gagatcaaga acaccgggac
     1921 gagcagcgtt gccatcagca gggccgaact gtatcccaac gggatctccg tcatcaacga
     1981 caggacctac gactcggtgg gcaccatcgg tgccggcaac accatgtcgt tcacattcac
     2041 cgtgcaggcc aataccgcgg acggcacata ctacccggtc ttctacctgg atctcaggaa
     2101 tggcgggagc atccgctact ccatcccggt gacggttgag agcacgggga tccaggtcag
     2161 cgtcgtcgac gctccggaga cctacccggc caacagcaaa gacactctcg ttctctccgt
     2221 cggaaacccc cgtgagagca gcgtgaacgg cgtaaccgtc accccgggcg gcgaaggcat
     2281 caagattacc cagacggccg tcttcctcgg cgccctgaca ccggacgagg agaagaccgc
     2341 cacattcgag atcctggcgt cgcagtcgac cgacctcacc ttcgacgtct cctaccggaa
     2401 cgggaacaac gagcaccacg ccaccctgac cgtccccgtc gagatcggcg agagaacggt
     2461 cgaaccggat atggtggtca ataacatcga ggtgtcgcag agcggcagcg cgatcacgct
     2521 gaccggagac gtgaccaacg ccggcctgaa agacgcgtat tccgtcaagg tcaccgtcga
     2581 tagcccggcg accgcaaccg acccctaccc ggtctacgtc gtcggcggac tggagtcgga
     2641 cgacttctcg agtttcgagg tcacgtgcaa cgcgcagggc gcgtcttcct taccgcttct
     2701 cgtccagtac aaagacgagg acggtaaaac cttcagtgag accgtgacca tctccctctc
     2761 atccgcagga caggcaggta ctgccggtcg ggccccggct gacatcagca gcggcggccc
     2821 ccagggtgga agaggaggca tgggcatgtc gttcggcagt ggatttgggc agatcccggt
     2881 agtcgagatc ctcctcgtga tcatcggggg cgtggctgtc gtcgtcgcat ggcggaaggg
     2941 ctacttagga aagatccgtg accggttccg caaataatcc aggggaggaa gacgcatgag
     3001 cagtccggtt atcgaactcg aagatgttat gaagatctat cccctccctg cgggcgatgt
     3061 tgtggcgttg aacgggatat ccctcaccat caacgaggga gagttcgtcg ccatcatggg
     3121 gccgtcgggg tcggggaaat caaccctcct caaccagatc gggtgcctgg accgtcccac
     3181 gtccggcaac ctcttcctcg ccgggaagaa caccagggag atggacgatc gagagttgac
     3241 cagcctgcgg ctctcttcca tcggctacat attccagaag ttctacctca tcccgcttct
     3301 ctccgcctac gagaacgtcg agtaccccta catcatgaaa cacaggaaga acgacgatac
     3361 cggaagggtg cgggaccttc tcgcccaggt ggggatcgac gaaggcctct cggcccataa
     3421 gccgagcgag ctctcgggcg gccagcagca gcggatcgcg atcgcgagag cgctcgtgaa
     3481 cgacccggcg atcctcctct gcgacgaacc gaccgggaac ctggactcga agaccggcgc
     3541 ccagatcatg gaggtgctct cggatctcca tagaaagggt cgcacgatca tcatggtgac
     3601 ccatgaatcc gagatcgcgg catacgcaga acggacgatc gtcatccgtg acgggagggt
     3661 ggcatgatcg ggaaagatat catctttgcg ctctcgatga ggagtgtccg gcttcacttc
     3721 ctccgatcgg tccttgccgc cctcggtatc gtgatcggcg tcgtcgccat cgcctcgata
     3781 gggatgatgg gggcgaacat gacgctctcg gtcacggagc agctctccga catggcaaat
     3841 aaactcaccg tcaccccgta caccgggggc ggcatgatga tgggcggggg aggaggcggc
     3901 agggtcgtca tcggcggcgg ctcctcttcc aacgacgaca acgtgctcac tgaaaaacag
     3961 ttccgggata tcgagcgtat cgtcgcgaag tacgggaccg cctacaccgt caggtccgag
     4021 tccgacgaga tcgaggttgg gtcggagacc gggagagcga ccatctacgg cctcgatccg
     4081 gatatcatcc gcgagattct caccgtcgag gagggggagt acccgaagag cacgacctcg
     4141 gtggtcgtcg ggccgacgct tgccgaacgg ctcgacctca agatcgggag caagatcaag
     4201 atcggcgacc ccgacgaagg gtccacgacg accgtccggg tcgtcggcat cctcgaagag
     4261 cgggggatgt cgatggacct gaatacagat atggcgatca tcggcaccga caaactcttc
     4321 accggcatct acggcggcga gggggagtac gaccaggtca acgtcgtcgt gaacgactta
     4381 aacgatatcg atacggtcaa gacggcaatc gaagacgaac tcaacaggaa agaggatgaa
     4441 gtcaccgtcc aggacagcag ccggattatg gagaccatca cctcgacact ctccacgatg
     4501 acgacgttcg tgatggcgat tgccgggatc tcgctcctcg ttgccgcggt ttcgatcttc
     4561 aacgtgatga tgatgtcggt gaacgaacgg gtgcgggaga tagggatcct gcgaagcatc
     4621 gggacgcagc ggcccgagat cctgcggatg ttcatctacg aggcaacgat catcgggatc
     4681 gtcggagcag tcatcggcgc catcgcgagc ctcatcatcg gctacgtcgt cgtgctcggc
     4741 atggtcggca ccaccgagta cttcttcgcg gccgggagtc tcatctacgt tccaatggcc
     4801 atggccgtcg gcgccgggat ctgcatcgtc accggggtct acccggcatg gcaggcgtcg
     4861 aatctcgacc cgatcgatgc gttgcgggca gagtaagagc aggaagaagg atggccctca
     4921 tccttctccc caacccgcta gccgccgatc acggtctctg aaccgccacc gtctccgccc
     4981 gcccgatcag ttcccggagc accgaccgcg cgagcccggc attctcgtaa tccctttccg
     5041 cgatcacttc ccggatacag gccggcacat cgacgaaccg ctcggcctcg cggatctcgg
     5101 ctgcgagcgc cgcgagatcc ctcttctccc gcatccgctc ccggtgccag gccgcggacc
     5161 cccggtccgg gtcgatcgcg aggaccgcct cgtagcaggc cgccgcctca tcgtacctgc
     5221 acatgctccc gagcacccag cccctgctct gccaggcgag gatatggtcc gggtccagcg
     5281 tgagcgcctt ctcacagcag gcgatggcat cttcgctcct gccgaggtgg gcgagagcgt
     5341 atccctttct ctgccaggcc gtcggatctt cgggatcgat ggcgagcgcc cggtcgaaac
     5401 aggcgaccgc ctcgtcatag cgaccggctt tgaggagcgc aaaccccttc cgcctccata
     5461 gaacagggtc gtcgggggag agttcaaggg ctttgtcgta acagacggct gcctcgtcgt
     5521 agtttctggt gttgcagaga gcctgacctc tgtggtacca tgctccggca ccctcttctc
     5581 tagtcgccat cggacctatg ggggagacca acccataaga acctaacccg taccgggaac
     5641 gtttaagacc ggtcgaacga gagatagttc tattatggca gataagattc tcgttgcgta
     5701 tgccacccga tacggctcga ccgcagaggt ggcggaggcg atcggcgatg aactccggaa
     5761 ggcaggcgtt gccgtcgatg tcttgccggt gagcgatgtc cagaacctct cctcgtaccg
     5821 ggcggccatc atcgggagcc cgatctacat ggggaaatgg ctcccggagg cgcaggtctt
     5881 catcgagcag aaccagcagg acctgcgcag catcccggtc gcctacttta ccgtggggct
     5941 gacggtcgcg gacggggggg ccgagatact caggaaggcg gaagcatcga tggaccaggt
     6001 caggatgctc gtgaaccccg tagatatcgg catcttcccg ggaaaactgg agagcggcgg
     6061 cctctcgctt gcggaccgca caatcaccaa gctcatccgg gcaaagacag gagacttccg
     6121 caactgggag gccgtccggg cctgggcgga gacggtgcgc tcgatgctcg tccccgcgtg
     6181 accggtccct gccgcactcg cggcagcaaa ctcacagggc atgcactaca acccctattt
     6241 ctcattccga ccccagaact ccgggaaaag gtgcttatag gacgaccgcc catttatctc
     6301 tgccgggctc gcggg
//
LOCUS       DAJB01000154            9153 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_74900, whole genome shotgun sequence.
ACCESSION   DAJB01000154 DAJB01000000
VERSION     DAJB01000154.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_74900"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    5859..5884
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1822 a         2828 c         2932 g         1545 t
ORIGIN      
        1 gtctccctcc cggcaccaac cccgacacca ggagggggag gtggcggcga cgattcgtcg
       61 tcctcctgga tcaccgtcac cctcgaatcg ggtacgttta acgtcaccgc ggcgaacagc
      121 gggaagacct acacggtcga ccggcggact gcactggggg ccctcgatgc agcgggaacc
      181 ccctatacca tcgacgactc gtactaccag gagtacgggt cgctcttcct caactccgtc
      241 aatggcagag cgggtgaggg gatgaaggga tggatgtacc aggtgaacgg cgaaagcccc
      301 ggcgccggcg caaatgcgta tgcggtcaca aacggcgatg aagtcgtctt cttctggagc
      361 gaggggatga gttcaacccc ggcgacatcg ccggacgtca tctcgatcag ggttgtgatc
      421 ccggcatctt ccggcgactc gggatcgagt tcgtcgggcg acggttccgg cgtttcgggc
      481 accacaacct cgggatccgg tgagccggga acggccccgt cattcttcct cggactaccg
      541 gccggagccg tcatcgaact cggagagtgg ggtcaggtct tctcgtttga caccggcagg
      601 gggaacacgt ctgcggagcg ggtcaccgta tcggggaact cctttattat caggcggaac
      661 gggctgaccg tggttcttgc cgcccggaac atcaacgaac aggacggtag ggctacaggt
      721 ctgatcgaga gcgtcacggc atcgatcgag cccgtcacgg ccaatatcag cacgatgggc
      781 atcgtcaccg catcgctgga cttaaacctc accggcatac cgggagacgg ccgcatcgag
      841 atctccttca atgagacacc tgatccgggt gcggcaagcg ctttccacct cgccacgaca
      901 gagaacgacg aggaaattgt ttctattgcc tataccatga ccgtcacgcg gaccaacctg
      961 gagaacggtg aagatatcgc cggagcggtg atccggatga ccgtaagccc cacatgggtg
     1021 gaagagcacg gcggcgtgga tgccgtcagg atcgcacggt ctgcggagga cggcacatgc
     1081 gagatactcg atacccggct tgccggaacc gacgggaacg gaaacctgat tttcgagggg
     1141 atctcacccg gcggcctctc ggtcttcggt ctgctggcgg tcacggcaac gcctgcggca
     1201 caaggtaccc cggcgatcac tcctgtcgct gccatcccca atacgcctgc tgcaacaggc
     1261 gcatcaccgt ctctctcatc accgctcatc gtcgtcggcg tgggcgcggc cctgatcatc
     1321 ggagcggcat acctcttcat cgaacggagg agaaagcaat gaagcggatc ggcagcatcc
     1381 tgcttatcct cctgctctgc gtcgcctcct gcggcgctta ccccctgaac acgggcgacc
     1441 cggccatcca gacctccctg gactatctcc gcaactgcca gaaggatgac ggcggtttcg
     1501 gggaagccgg gcgcgagagc agcccgggca cgacgtcgtg ggcgatcatg gcagccgtcg
     1561 ccgcgggcga agacccccgg acatgggtga agaacggcaa ctcgaccatc gactacctcc
     1621 ggtcgatgaa cgatgagatc ctggcaaaag gagggaccgt cgatatcgcg cgcaccatcc
     1681 tgacactggt cgcggtcggg gaagaccccc gtaccttctc cgggacagac tacgttaccg
     1741 aactgaaatc ccgggtgaaa cccgacggcc aggtggggga ccacgtctac acgaccatct
     1801 ggacgatcat agcccttgct tcggtcggcg aagacaccgg tgcttcggtc aggtggctaa
     1861 tggcccagca gaatgccgac ggcggcttcc cctggacacc gggtgccgag agcgaccccg
     1921 acgacacggg cgccgcactc gaggcgctcg cggcggcagg tatctcccac gatgcagccg
     1981 cggtgcagaa cgctctcgca tacctgaagg gaatgcaaca ggagagcggc ggtttccatt
     2041 acgggggaac gagcgcttcg aactccgcat ccgatgcctg ggtcatccag ggtatcgttg
     2101 ccgcgggaga caaccccgca gggtggagag tgggtgaaat gagtgtggta gaccacctcg
     2161 ttggcctgca gaatcccgac ggctcgttcc gctacaccgc gtacgtgacc gacagtccct
     2221 gcaggatgac ggcaagtgcg gttcccgcac tccttggaag accatacccg atactgcctt
     2281 catcggccgt acagccatca cccgtcgcag ccgcaccggg cgtcacgatc gctgccgcaa
     2341 ctgcaacccc ggcaagcaca cccattccag caacgacgga catcggcgag agaaccgtca
     2401 cggtgaccga cgacttcggt gagatcgtca ccatccgggg tatcccgcag aggatagtct
     2461 cgctcggtcc ctccaatacc gagatcctgt atgccctcgg cctcggcgac cgcgtcgtca
     2521 gtgtgaccga ctactgcaac tacccgccag aggctctcga taaaccgaag gtcgggggat
     2581 acagcaccat caacatcgag aaggtgatcg cagcagagcc ggacctggtc ctcgccacgt
     2641 tcggcaacac cgaagacgcg gtcgaccgcc tgcgaacgtt cgggatgacc gtcgtttccc
     2701 tcaacccgct gacaatcaac gatgtcctgc gggacgttga actcgtcgga gcgaccaccg
     2761 ggcaggaaga gcgggcagcg gcgtgcgtgg aggatcttca ggcccgcatt caggcggtga
     2821 ccgataaaac ggaggccctg gtagaaaagc cgtccgtcgc ccatgtcgtc tggtacgacc
     2881 cgatctgggt cagcggcggg gaaacattcc aggacgaggt gatcacgatg gccgggggaa
     2941 caaacacatt caattcaatt ggggggtggg gtattgtcgg ccttgaagag ttcatcacca
     3001 cagacccgga gtacatcttc gtcagttcgg gcaccggcat gggcgatggg gggtacgata
     3061 tcgtctacaa ctacttcatg aacgagcccc ggatgcaaag gcttgatgcg gtcaggaacg
     3121 gacacgtcta cgtcgtcgac gccgatatca tcagccgggg aagcccgcgg atcgtggatg
     3181 cgctggaaga ggtggcaggg gatctccacc cggatctctt tgatacgggc accccgacgg
     3241 caaccccgac agtccagtcg ccgggtttcg gcgtggtccc gcttgccttt gcgttcctcg
     3301 ccgttctcct gatccgggtg aagaggtaag atggaccggt gcagaatgcg gcggaacctc
     3361 atcttaccca cgctttttgc agttgttgta gtcagcagcc ttgcatgtcc ggtcctggga
     3421 caggaggtct ccgtcaaaga gatctggcga tccggaatcg gagatccggt tgtggcggtt
     3481 gcactctcac aggacggcct gtacggtgcc gtcaccacag aagagagggt ctacctctat
     3541 gaccaaaacg gtacaattct ctggcgttac ccggtgtccc acagcaggag cgtggccatc
     3601 tcttccgaca gcgaacgtat cgtggccgga ggggaccatc tcctgctgtt cgaccggaaa
     3661 ggcaacgtaa tctggcgcta caagccggag tcccggatac agggcgtcgc cgtcgccgca
     3721 gacggacgta cgatctgcgc aggggccggt accagcctcc gggtcttctc cctggatgac
     3781 gggcaaacaa ccgccaacac ctcctggtcc tttgatacgg gaggcccggt tagaagcgta
     3841 tcgaccgacg gtgacggttc gagcatcgtt gccggtggcg attcggggaa tatatacttc
     3901 ttcagtggag acggccggct tctctggaac tacaggacgg gtagtcacgg cgtccgggtg
     3961 gccgtctcac gcgacggctc gacggttgca gcagcatcga tccagcgggt cacgttcctc
     4021 cttaaccgga acggaagact cctctggaag tctccgatgc ctgagatgat tacagatgtg
     4081 tcgatctccg gcgacgggtc aactcttttg ctggcgaacg ggagtatctc cgttttcaat
     4141 cgggatgggg aggctgtgtg gacgtacgtg acggaggaaa agaccaggtg cgtctccgct
     4201 tcatccgaca taacccatat cctcgccggt acactcgacg gaaccgtatg ggcattcagg
     4261 gtccaaccgg aaacctttcc tgccggcaca tccgagacat catcgccgga taccgccttc
     4321 actgccggac cgccgcaaac ttccgtttct tccgctccgg ggcagacact cccgcagcag
     4381 gggacggcac tctccccggc aacgccggtt accatgggtg cgtgctttgc cgcgctggca
     4441 ttgtggcgaa gacagaaacg gtaacctgat cccatgtcca caaagaattt cattggctgc
     4501 tccacctgct gcctgatgga ccggccgctc gaagaggcgc tcggcctcat ctccggccaa
     4561 acgggtcttg cggagatcct ctctgacggc cctcactccc tcttcctctc cgaagagacc
     4621 tgttattcgt tcgatctcag gtacacggtc cacgccccgg tcgccgatac caatatcgca
     4681 tccgacaacg agcacctgcg aagggcgacc ctcagggtta tgagggacct cgcggaggtc
     4741 tgcgaccgga tcggcgctga gcggctggtc atacacccgg ggcatgtatg gggagaagag
     4801 gtgcggggcg ccgcccaggc cgccctcgac cgttcgttga gcgaactggc cgcaatccag
     4861 ggagaccaga acgtccggtt cgccatcgag aatatggggg cgtgggatgt ctgtttcttc
     4921 agggagcccg ggttcctcga tcgtcttgcc gatcttgatc tcggcttcac cctcgacgtg
     4981 gggcatgccc acgtgaacgg caacctggtg ccattcctgg aacagggagg agcgatccac
     5041 gttcacctcc acgacaactg cggcagtcgg gacgaccacc ttgcatgtgg ggagggaagt
     5101 atcgactttg ggcatgtaat ggcggctctc cccccggacg ccacgaaggt catcgaaccg
     5161 gtctcgtttc agcagtacgg gagaagcctc gaacatctgc gaacgatcct tccatgacgc
     5221 cccaggacct cagggatacg aactccgccc gatcagccgt tcaaccgccg cgcgaaactc
     5281 gcggaactgc cgtgagacct ccggcccggg cgagcccggg taggcgtttg caccccgcac
     5341 gacggtgctg tgcccgtcca tctccatccg aaggtgccag taggtgacat cgcagcagga
     5401 gtaggcggag acgtactccg gctcccactc ccatacacca agcctggttg cggtctcccg
     5461 gaaccttacc cactcctcct ctgtgggcga cgcctccatc actaccccgg agtagccgcc
     5521 ggcactcgcc cactcgtaga ggagccggcc gtcgacgaac ctgacgtaga gggatggccc
     5581 gacgctcccg ccgaggtaaa actcgaacgc tgccggaagg gaggtatggg gagagggaga
     5641 tgcaccgtcg cacataccag gctatacgac cagcacggcc aaataccctg cgaccacaag
     5701 gcccatgcag cagatcccgg cgacgtcggc cctgcccggg acgaggtcgc gcatcggcgt
     5761 ccgcctgccg gagcggtagc cccggaggtc gatggtcaga ccgaggaccg tggcctttcc
     5821 gagggagttt gagaccagcg ggatgatgat ggggaagann nnnnnnnnnn nnnnnnnnnn
     5881 nnnnacaggc atccggaggc ggtccatgac gtggacgagg tcgcgcggct gggtggatat
     5941 gaccaggagc tggaacgcaa agagcatcgc ggcgaaccga agcgacatcg caagcgcaag
     6001 gtcgatcgct cccaccgtga cggggaaagc cccgccgatg accgggaccg acggagggat
     6061 cagatagccg aggatatccc cgctccggat ggtgaggatg gtgagggcaa gcagactcac
     6121 cacgagcgag atcaacaggg ggacctgacg gaggaggtcg cgggcgagcc cccccactac
     6181 cgctacggct acgaccgccc cggcaaggac cgcaagcatc agggtatcgc tcgtcagcac
     6241 cgcaagggcc acgacgacgg cggcaaagat cagtttggtg agcgggtgga ggcggtgatg
     6301 ccggcacctc gagcacctgg acaacgacgg cgttgaccgc cgccgtgatc aggacgatcg
     6361 gcacggtgac cgcgaggaac gcctcgaggg tcccgaacct gccgaggacg gtcggggcca
     6421 cggtgatgat ggcgatgagg atgaaggaga acccgctcgc gaaggtggca acgagcgtcg
     6481 tcaccgccgg ggcgagaacg aaccggtctc tcagcaccag gtaggtcgcg agacagacga
     6541 ccgccccgat aggttcgctg agcaggtttg cgggcgggaa gagcgagtgg ctgagaaggg
     6601 cacagacggc acctgccaca acgccgatcc cgagcgcctc ccggaacgtg ggcgcgatga
     6661 ggatgatggc aaggctatag aaggcgatca caaggttcga tacgatggag ccggggatca
     6721 gtatggacgc cagcatgaga agacctccgg tcttcgaggc ctgaagagtc ctccgggggt
     6781 tctttcggag gcgataggcg gggtagaagg ccccgccagt atgctgcaca tcatcgtata
     6841 aaacagtaca ttagtgcata tgtatttacc aggagcaggg accgtgggcc gggcatatca
     6901 ccggcgcgag agcaggagca ccgcaacaag caccgcgccg gtcgtcgtga ggacggctgc
     6961 cgcaggtgcc gctgccgtcg gcgtgggcga ggctgtggct gccgggatcg tcggggtccc
     7021 cggctccgtg ggcatcgtgg cgggtggagt ggcggtcggc gtcaccaggg ttcccggggg
     7081 tgtcccgagg gtgacaggaa tggtcgtgta gccgtggccg gagaggtttg atcggtctac
     7141 gggcgcttcc acggcgtaaa cggtgtaggt tccgggatcg agcccggcgt cacccgtcct
     7201 ccagcggtag gtccagccgt catcggcacc gactgtcacc gtcgtgaaac tggccgggtc
     7261 cccggaccgc acaggcgtac gcgggtcttc aagccgtccg ccggtggacg ggaggttcgg
     7321 gccggtcacg aagaggtaga cggtatcgct ccctgtgctc gtccccgcga tcgtgacctc
     7381 gcttcccgga tacaccgtga cggcggaggc agccggagcg atgacgaggg cgatgaggag
     7441 aaggataccc ggaatcaggg ctctatcagg catatggttc atcgtcaccc gatcgacccc
     7501 ggaggtgatg aacctgttct tgtcacataa gggcagcacg tccgcctccg gcatacgcct
     7561 ctacgagtag ccgggtgctc gccttcgcct caccgcctcc gggcacataa tccactccgt
     7621 cgagggcggc aacaatcgcc cggacggctt tcgcatgcga cggcccatag cccccttcca
     7681 ggacgagggc aagagccgca ggactcgcgg ccgccagcat ccccgtcagg gctccaaagt
     7741 cgtccggccg gaggaggatc gaaccaagcg ggtcgtcgaa gagcgcatcc tggcctgctg
     7801 agaccaccac gaggtccggc ttgaggcgtc ggattgccgg gatgaagacc tccccaaaga
     7861 ccagagcgta gtcggcgccg gtcgagcctg ccgccagcgg agcgttgatg gtgtacccga
     7921 agcccgggcc ggcaccctgc tcatccggcc acccggtccc ggggaagagc cccgcctggt
     7981 ggaccgagca gtaaagcacc cggttcgagg cgtagaagat cgcctgcgtc ccgttcccgt
     8041 ggtgcaggtc ccagtccacg atcgccaccc ggtcgaccct gcggagcgcc cgggctgccg
     8101 cgacggcagc attgttgaag aggcagaacc ccatggcccg gtccggcacg gcatgatgcc
     8161 ccggcggacg gaccagggca aaagagtgtt cgccgtggag cgcccgctcc accgctacag
     8221 aggctgcccc cgccgcatac agggccgcat cgaacgaccc acgggtgacg tacgtatcag
     8281 gatcgaggta gcagacccgg tcggggggac actccctgca gaacgagcgg atagccgcga
     8341 tgtgctggtt cgtgtgcacc cgtgccagat ccgcgggcgt cgcctgttcg ggggcgatgc
     8401 gacgggcatc ggccggcaac ccggcgaggg cggcctcgag gcgtgcctgc gactcggggt
     8461 ggccgggatc gtcatgagcc cggaagacat cccccgtcac gaccgagtac ggcatggtga
     8521 tcgtttgtgg gttgtcggcg ccggatatga atcttgcctg ccggatgggg accgggggca
     8581 tggtggcagg ggatccgacc ttaacgcagt tggcggggct gttcccggtt caaccggcat
     8641 gcatgaggtt cccgaccggt ccccgctgac catagccggg tgccctgagg taaagtgatg
     8701 aaaggaatgc cgttcgtgta ccgaaggtgc aactcaacga ggaggagatc caacggccca
     8761 gagtatccgg agaacgaact gttgaccctg attgttatgc cgataacata ggcatccggg
     8821 gaacataccc cccatcctgt tccctggaga gggccggcac gggggtagtg ctccagcatg
     8881 taactgataa gtggtctcag agaatccaac cgaattacgg tgtgcaccaa cccgcgttca
     8941 tgacaaaaac taacctgggg agaagacttc ccttatcggg ctctggtgaa gccctattcc
     9001 ggctgtcatt cttccctgac gagattgatg ccggaccctg cctgtccctg agttcgacct
     9061 ttcgtatgaa aatgggtcac ctacatattt tcatccggta tgcctgtggc cccacgggca
     9121 tcatgtggtt tttctcaagc atgtcctcat gcc
//
LOCUS       DAJB01000155           10280 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_5324, whole genome shotgun sequence.
ACCESSION   DAJB01000155 DAJB01000000
VERSION     DAJB01000155.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_5324"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    6697..6757
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7853..7862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1792 a         3207 c         3211 g         1996 t
ORIGIN      
        1 gttttaaccg ggggtagttg actcaatgtc gcggaatgtc gtacggtatt acaattaagt
       61 atacgtctgc tggataggtt aggatatgag actgcgtgta gtgctcgaac ccagtgagga
      121 tggggggtat acggtctatg taccaagcct tcccggctgt atcagcgaag gcgatacgag
      181 agaagaagca ttggataata tcagggaagc tattgagctg tatcttgaaa ccgttgagga
      241 tgatctcgtt ttcagcgaaa cagccgagca ggttgagatt gctgtatgag caaggtgcct
      301 gttcttgatt atgacagagt cgtcattgct cttcaaaggg acgggtgggt tgtcgtccgt
      361 acacggggta gccacattcg actgcataag catctgccgg ctaaaatact gaaactgaca
      421 gtccctatgc acaaaccaat caagcgctca acgctgtcgc acattttaaa acaggccgat
      481 atgacggtcg aagaattgaa tgatctgctc taggccggcg catgtgagcg tcaactccgg
      541 caggctacgt tgaccgacac tcgatctcca caggaccggg ctctcctggg gggatgcccg
      601 gtctgccctt cacgagaaaa gggctaaccc tcccccctac ccgatcagct tcaccagccc
      661 gtgccggggc tccagcacct cgcagccgct ccccgcctat cacgtcacct tgatatagcc
      721 gtgccggggc tcgagcaggt ccccgccccg tttcagctgc tcgatggtgt gctcgatctt
      781 gtcgcgggag aagccctgct gcaccaggat atcgagcact tcatcgagct ttgcctggcc
      841 gccggagtcg ccggagatgt tccggatcgc ctccttgacc gaccggatga tgtcgcgctg
      901 cgacttcatg acgccggtca cgagcttgtc gatatcgaag ctccccgtct cggcgtcgta
      961 ggcgacctgc cggagacaga cgtccacgat cttgaggatc cgatcggtat cctccgtatc
     1021 gatggtggtc gagaggcgca tccgggcgct cgcctcagca aggcggacca gcgcctcgag
     1081 ctgccgggcg gtgaccggga caggcttgtt cccgctcgcg aggtcccgga gccgcatata
     1141 gtaggagatc agcgcatcct gggccccgtc ggagaggatg gggaaacagg tccgctttgc
     1201 atacgcgatg tacttccgca ggagcgtcgg gtcgatgtcg gggatgacgg gcgcaagcgc
     1261 ccgctggatg tacgcgtcgt cgacgtcggg cagcggcgcg tatgcgtgct gcttgatcag
     1321 ttcgccgacg gaatgagtct tgatgatgtg gtgggcgatg gccccgtcgc gctcggcctc
     1381 cggctggtcg gtcatcacga agatgaggtc gaaccgcgag aggagcgaag gcggcatgtc
     1441 gatctgctcg ccgatcggga cgaactggtc gaaccgtccc agtttcgggt tcgccgcacc
     1501 gaggagcgca caccgggact ttaaggtcgc ggtgatgccg gcttttgcga ccgagatcga
     1561 gttgccgcac cagatcggga tgccgtttcg ccggacatac agaatatggt ttgggacctc
     1621 gaggcagtag atcgtgccct tatacgggcg cgtctcgatc tgccgctgcc cgttcgagtt
     1681 gacgttgggc tcgttctggc cgtcgcggat gaaggtcacc tggaagatat cgtgggtcgc
     1741 ggtcgaggtt ccaccccgcg gggtgggtgt gacggtcccg gcctcccgca tgagatggac
     1801 gttcccggac caccccttct tcagcaggag ttcggtgacg tcgtcggcta gaacgttcga
     1861 ggaggtggtg tacatcgaga ccccggtcgt cctgtcgacg tagccgtccc cgagcatcag
     1921 cgcgtcgaga agtatctcga tctgctccgg ggggaggcgt ttggcgtagt cagggacgta
     1981 cttctcgtga cattttccga acggcgcaag gtgtgccgcg agctgctttg cgttgattgc
     2041 aaagtttgca ccgttgtacg aaaagcggaa cggcagcctc tcaagacact gccggatcgt
     2101 ttcgaccgac tcaggaatct tctgcgatat gatcacgcgg tagggaacct gcgtctggtg
     2161 atgccgctgg acggaccctt cggagaggaa gtagcccagg aactcaagcc agtcatccat
     2221 tccgatgcgg atgggctcgg tgagctttcc ttttgagttc tggttcgcga acttgagaac
     2281 gggcgggatc tcgtaggtct cctgccgctc ccccgtccat acggcgttct tcttgaatcg
     2341 catcctttta tgggtgggga cctcgtccat gcggatgagc ccgaacccct cccactcgtc
     2401 ggcccgccgg ttcaggttga cgtacatccg gtggttcggc gtgaccgcga ggtccacctg
     2461 ccgcgacttg atgtaataca gttccccgtc gtaatccgat gccacaaaac cggacggagc
     2521 tgcgtactca agcctgccgt cgggagagag cgtcgcgaca cggtcgtctg aggttacatc
     2581 ccggaatagt ttccaaccgc cctccgtaag gacttcggtc acgtcgtcgt agcactgctg
     2641 ctccattgcc tcatggagcg cgctccggtc ctctttcgcc atcttgtcca actcatcgac
     2701 ggctgcgatc cccatatcgg cgaggaccag cgcaccggcc tcgagcgtcc agcggccgtc
     2761 gccgaactcg tccttgacgg ccgtcgccgt cagccccgcg gatgtcgacg acttgccgct
     2821 cgtgtagatc ccccgcggcg agagtttcac gacgtaacgc aaaatttgac tctttgcgat
     2881 acccgggtcg ccgaccagaa ggacgtggat atcgccgcgg aggcgggagc cgtccggcat
     2941 ctccttggcg atgccgccga agagctggag cgcgatcgcc tctttcacgt cgtcggtgcc
     3001 gtagatcgtc ggcgcgatcg accgggcgat cttcttgtag atcgagggat cgcgggcgag
     3061 cgccatgatc ttcgtctcgt cctcctcggt gatcgagacc tcctcgaact ccttctcgcc
     3121 gacctcgatg gagttgcact ccaggtagat gtcaaagagc gtgctcttct ggccggagtt
     3181 gaccctctgc accgatcgca ggatgccgtt caccaccacc cggtcgcccg gcgcgaccat
     3241 gccggtgagg tcgtcggtga catcgatatc gagcgtctgg ggctgttcgc cgccccggag
     3301 accctcgggc gactcctgga tccggagttt ctgggcgtcg acgaaccggc accggttcat
     3361 caccaggtcg agacgggtct tgcgctcgca gttcgggcag aagtcaggct cgtcgaaccg
     3421 cccgtagccc tgggggaccg gctcggtgtt cttcccgcag gagcggcacc ggaataccgc
     3481 gctgacgatc cgggggcgca cctcggtcgt ctttctgagg atcccctcga tgccgatgaa
     3541 cgtgttgatc tggtcggccc tgatatcccg gacgtcggtc ttctgcggca ggttcgtgaa
     3601 ccggatgttg atctgctccg ggccctggct atctttcagt ttgacgagtt tgttctggac
     3661 aatcgcgtcc cggatgtccc cgagcacctt gcccgggctc cgcaggagct cgaatgcgag
     3721 cctgttattc aggatctttc gaaaatcgat ggtgagcgac cgcttatggg ggtactcgcg
     3781 ggagagctcg gcaagctccc gcttgtactg cttctttaag aatttcgtcc actcaccgac
     3841 gacgtcggtg acctcaacgg ttacctcttc agtcacggca gacccctcac cggtgctgcc
     3901 ctgcggcgag gacgaaccgg gcgagcagcg cctccatctg gatgtcgctg cttgctcctt
     3961 cggagagacg gaaatcggtc tcgccgaggg catcgatcag tctcactttg agcgtccggt
     4021 tgatgtcgcg ctggaccagc gcccggtagc actggttgat cagctcgttc ggggcgatgc
     4081 cccggccgcg cgtcagttcg gagagcgcct gttccgcctc atcgaacttt cctgctatcg
     4141 agaggtcgag gagctcgttg atctccgacg ggcgggccgt tgcggtgatc tcgtagatca
     4201 tctcctcgtc gatggtgggc cggaggatgg cggcgccctg cagggcgttg atcgccttcc
     4261 tcatatcccc ggaggcgaca tagacgatgg cgtcgagcgc cccctccgtg atggtgaggc
     4321 cttcaactgc ggcaatcctc cgggtctcct cgatgaccgc ctcacggtcg agcggcctga
     4381 accggtagat ggcgcaccgg ctctggatgg ggtcgatgat cttcgaggag tagttgcacg
     4441 agaggatgaa ccggcaggtc cgggcgtagg tctccatcgt cctccggaga gccgcctgtg
     4501 cgtcggtggt gagcgcgtcc gcctcgtcta agaagagnnn tgccaggagt cgccgaagaa
     4561 ctcccgcgcg agcgccacgg cggcggtcgt cttcccgatc cccgcgctgc ccgtgaagag
     4621 aaggtgcggc aggttgccgg tcttcacgta ggattggagg cgcaccacga tatccttctg
     4681 tccgaccatc tcatcgagtc ttctgggccg atacttctct atccagatgg tgtggttgcc
     4741 gtccatttgc ataacgtttg gtgctcgcgc atcgtaaaag catcgacatc tcttccggtg
     4801 atccttttta gagggttgag ggcgaagaga taggtacaga gaccagggaa atatcacgag
     4861 tttgcgtgga gttacggcca gccgggactc ctgcaccgac attccttgtt cctttcctga
     4921 ggcggacgga ttatggaacc tgctggacgg gtatttgcgc gggagttctc cgccgcgcgg
     4981 catacggtgg actccgggga cggctcatac gtggtgacgc ccgctggcgc ctggtgccgc
     5041 cgcctcttca tcgtcggcgc cctcaccgag aagaggggga gcggcgacgt catgcaggcc
     5101 cggatcgccg accccaccgg aacgttccgg gtcacggcag actggcagag cccggacgcg
     5161 gtcgagacgc tcggctacat cgagccgcct gcgttcgtcg ccgtcacctg ccgggcgacg
     5221 ctcaccggtt ccgggtccag ggcatacgga gtgttggacg ccgaagcgat caacgtggtg
     5281 aaccgcacgg ttcgcgacca ctgggtgctc gcgaccgccg gcgcgatgct cgatcgcctc
     5341 gaagcactcc gggacggcgg cgacgagcgg gccgcgcccg ctctgcggct ctacgggatc
     5401 acggacgagg atattcgcga catggccggg atggtgcgga cggcgcttgc aagcatccgc
     5461 ccggatatcg cccccggcga tatcgagccc gtcgtcgggc gtgaccttct ccttaccgcc
     5521 ctcggtgacg agggaggggc ccggggcatc agtctcgacg ccgccgtcgc cgcgggggtg
     5581 cgctccggcc tctctgcccg ggacgcaaag agggcgctcg aagaactcat cgccgcaggg
     5641 gaatgctacc tgcccgcatc gggtatgata aaactgatct gacacgatat gcaccttcat
     5701 ttcatcgaga acggcccggc cctcctgatc gagcaggacc agcgcgtgct ggtcgtcgcc
     5761 gacctgcaca tgggcatcga gtcgggcctc gaacgtcacg gcgtccacat cgcgagccgg
     5821 agcgccgccc gggccgaccg ggtgatcgcc tgcatcgagg aagcaaaacc cgaccttctc
     5881 ctcctcctcg gggacatcaa gcacaacgtc ccggtcacaa gcaggcagga ataccgggag
     5941 ctccccggcg tcctcgagag gttccgggac cgcgtgccga tcgccgtcgc ccccggcaac
     6001 cacgacgggg gcatcggaca gttcctcgac cccggagaac tcctgccctc cgggggtgcg
     6061 ctcatcgacg gtgtcggcta cctccacggc cacacccacc ccgcccccga actccccggc
     6121 cacctgatcg tcctcggcca ccaccacccg gtcgtctcgc tcgtcgactc cgtcggatgc
     6181 gccgcacgcg cccggccggc ctacctctac tcggggttcg aagggccttg cctggaccgc
     6241 acccggctcc tcttcgtccc ggccttcaac gagtttgccg gcggtatcga cgtcgggcgg
     6301 ctgcacaaga gcgggcttgg tcccctctcc cggtgcatag acatgaagtt cgcggaggtg
     6361 tttttagcag atggcacgta cgtcggctcc ccggccacgc tctgccccgc aggcaacggc
     6421 tgacctcctc gacccgaggg tgcgggaggt cgtccggaaa cgtgggtttt ccgggttatc
     6481 cgaggcccag gagcaggcca tcccacggct cctcgcggga gagaacctga tcctggtggc
     6541 gccgaccggc acgggaaaga ccgagagcgc gatgttcccg gtcttcgacc ggctcctcga
     6601 gaccaccggc gccgggttca aggcgctcta cgtaaccccg ctccggtcct taaaccggga
     6661 tatcctcacg cggatggagt ggtggtgccg ggagctnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag atccacgaac tcgctgacag
     6781 caagcgggga gcgcagctcg cggtggcgct cgaacgcctg gccgcctacg cgggggagtt
     6841 ccagcggatc ggcctctcgg cgaccgtcgg gaacccggac gatatcggcc ggttcctctg
     6901 cggggcccgg gcgttctcgc tcgtggaggt gccggtcgcg agccacctcg atatcagcgt
     6961 ccggtttgcc ggggaagact tcgccgccca gacgcgggcc gtcgcgggct gcctggatgc
     7021 gcccggctcc accctcgtct tcgtcaacac ccgggtgacc gccgaggccc tcgggcacga
     7081 actcttttcc cgcggcgacg tcgaggtcca ccacggctcg ctctcgcggg atgtcagggt
     7141 cgatgccgag gagcggttca ggaagggcga ggtccggacg ctcatcgcca catcgtcgat
     7201 ggagctcggc atcgatatcg ggcatatcga gcacgtcgtc cagttcggct cgccccggga
     7261 ggtctcgcgc ctggtgcagc gggtcggccg ggccggccac cacctcgatg ccgtctcgcg
     7321 cggcaccgtc cttgcgacag ggtttgacga ccttttagag tcgctcgtga tcgcacgccg
     7381 ggcaaaggcg aacgagtgcg agcggatcgt cccgcccacc ggcgccgcag acgtgctcgc
     7441 gaaccaggtc gcggcgatcg cggtcgaata cggcgagatc gagatctccc gcgtccggga
     7501 gatgatcgag cgatcgagcg tctttgccag ctcgcggccg ctcctcgacg ccgtctgcag
     7561 ccagatggcg gagcaccgcc tgatccggct cgacggctcc cggatcatcc ggacgggacg
     7621 cgcacggcgc tacctcatcg aaaacctctc catgatccac gacgagcgga agatggagat
     7681 cttcgatatc gtctcgcgcc gcacggtcgg gaccctggac gagtcgttcg tcctcggctg
     7741 gatgcacacc ggcgcggtct tcatcaccaa gggccagctc tggcgggttc tcgagatgga
     7801 ggggggcagg atcacggtcg agccggccac cagggcgaaa ggtgaggtcc cgnnnnnnnn
     7861 nngttcccgg tgcgatcgcc tttgcggagc ggtcgcttgc ccggatggac gggggcaact
     7921 acccggtccc ctcggaccgc ctcatcaccc tcgagaacgc ggacgagggg gtggtctgca
     7981 acgtctgcgg cgggcataaa gcaaacgaga cgctggcccg ggcgctctcg gtcctcctct
     8041 cggcccgcta cgggacgacc gtcgggatcg aggtcggcgc ctaccggttc ctcctgcgcc
     8101 tgcccgacga cgtccgggcg cttgaggtgc aggagaccct cgccgcgctc gaaccagcgc
     8161 acctccccgg aatcctgaaa ctcgccctga agcggacggc gctcttcaag tggaaacttg
     8221 tgcaggtggc caagaagttc ggcgccatcg atgcggacgc cgactacgag cggttcagca
     8281 tccaccggct catcgacctc ttcgacgaga ccgtcatcgc ggccgaggcc taccgggagc
     8341 tcttcagcaa ctccatggat gtggacgcgg cgcaggagat cctctcaggc cttcgcgagg
     8401 gccgacttgc cgtcaccaca ggaaggctga gcccgctcgg gagcgagggg ctctcgtcgt
     8461 caagggacat gatcccgccg ccattggtcg accaggcgat tatcgggacg ctgatgcggc
     8521 gccttgagaa ggaggacgtc gtcctcttct gcatgcactg ccggaagtgg aagagccgga
     8581 ccgtcgtcga gcgggtcccc gaccggccgc aatgcccgct ctgcagcgcc cgcctcatcg
     8641 cggcgctgaa accctgggac gagcagttca tcccggcgat gaggaagaag aacaagtccg
     8701 aggaggagca ggcaaccgaa gtccggttct tccggaacgc aaacatcgtc ctctcgagcg
     8761 ggaagaaggc ggtgaccgtc ctcgccgcaa agggcgtcgg cccggaggcg gcgtcccgga
     8821 tcctggccac cctgaccgag ggggacgcct tctaccggga agtcctcaag gccgagcgga
     8881 attatgtgaa gacgcatagg tactggtagg gttttattca ggcgagggag accggagttt
     8941 tagacaaacc cggtaaactg gaccgtggga gtatcgccac caggggaggg gctgacgggg
     9001 aggggggagc atcccccctc ccctgtctct actcgtcaac tgacgtctac aacccccacc
     9061 ccacccggcc tgcggcctcc tcccccgccc cccggggcgg gggcagtgag tggcgatacc
     9121 cccacgaaat ccgtgcgtga gagttgcggt gattcaaggg acgacgtttt gcacactcca
     9181 agtacgatta ccctcagatg cgagcaccaa aaagacccgc ttcaggcccg gatactccgc
     9241 cggcacatac gggcaggacg tcctgatagg cgaaccagac cccgactaaa aatcaggggc
     9301 ccctgccccc ggcaaaaaga acgtatcgcg aaaaacagtt tcacattata gtttcgagcc
     9361 gctgctccag gaaatcaagt cctctcttga tatcctcgat agtctttccg ccggtcagga
     9421 tgatcttgcc ggaactgaag agaagggcga cgatcttcgg gtcctcgatg cggtatacaa
     9481 gccccgggaa ctgctcgggt tcgtactcga tgttctcaag gttgagcgtg atgaccacct
     9541 tgtttaagtt gatgtacctg cctatgtcgt acgaacagac gatgttcgtg accgcgacct
     9601 gcggctcatc gtaggtatcg acgccggcat cccgcagcga ttgcatgatg agctcaaggc
     9661 cgtcgtggag atcctggttg ttcctgatgc cggtgagcac cacttttccc gaggagaata
     9721 tcagggatgc gatcttgggc ttctcgatgc ggtagaccgc tccggggaac ctcttcgtat
     9781 tcagttcaca gttttttatt ttatccgata ccatttcgag gtcgatcgag cctgcaatcg
     9841 ccccggaggc aacgatgttc tcaactttca gtgactcgta tcccttctca gccattgata
     9901 taagtatcgt tttttgaacc ataatactaa tgggtaatct ttatgcctgt tccggatatg
     9961 gtcgaatagg gagaggatgt taccatcctc cccccttcca ggaactgtac gtgcgccttt
    10021 cagcgcatac agctcgcgca tttcactacc ccgctaaggg cggcaacatt gcgttgcctt
    10081 tgcaatccgg gtctgtagcc tattaacgtc cgaactcacg ttgtcccagt caatggagtt
    10141 ccattgccgg gcgagttccc tgtcagtatg cttctcgccg ttcggcgtcg ttgaatgaca
    10201 tactttcacg ggtatacctc ccttctttgc tgtgaaacac ctgtcccacg tgggcgccct
    10261 ttcgggccga ggtaagaacc
//
LOCUS       DAJB01000156            6292 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_112946, whole genome shotgun sequence.
ACCESSION   DAJB01000156 DAJB01000000
VERSION     DAJB01000156.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_112946"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    4604..4723
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5275..5386
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          926 a         1826 c         2008 g         1296 t
ORIGIN      
        1 gttccacctc gtgatgttct tcgcctctgc cggggccagg tcgaggagtt cggggtgctc
       61 tgcgagcgcg gcgataatat cggcgaggtg ccgcttcatc cgcgcccggt aatagatgtc
      121 ctcattgagg agcgtgagtg tgacatcggt cgcctgtcgc atgagctgtt gcttcctcgc
      181 ggcatcgttg tcggtgtggt tctcgcggat gctctgcttc atcacgcggt tgagtagcgc
      241 gaggaaggag tcgtcgtaga caaaaaccgc atcagggtct ttcttcgccg cccggtatgc
      301 cgcgctgttc ccggcgcacc gatatttcag gaacccctgc cacgcagtgt ctaggagttt
      361 ttcgaggacg gtgttcttga tcgtcttctg gaaaaacatc cccagtcccc ccgcatagca
      421 gttgtgggcg aacccgaccc ggggcgcgtt cgtgagcgcc tgagcactct gcgggtcacg
      481 ggcgatcatc gcctgcttct cgtcagggct cataaactgg gctgcgaacg attcggacat
      541 cctcagccct catagtagtg cggttcgcgg atcagcctac ccgagacgac gatgtttccc
      601 gccccggtgc cgaggttcgt aacccggatc aggtatttct tcgtccggtc aaggtagatc
      661 tccgggagcg tgccgtgtgc cgcgtcccct gccccgacat cacccatcgt gctctggtag
      721 aggatgtctg ccgggagtgc ggcggcgacg taggcgaccg gggccgcgag cgtgcctgct
      781 gccgatgcgg tcggcgtgtg gtagacggcg ggcgaactat tcatactcgg atcgccgttg
      841 cggttgagcc cgtatgcgat cagttcggtg cctgcggccg aggtcgtcgg ggactcatag
      901 atgccgaccg agcagtcgac gccggggtca acatccagcg cgagccggag cggcgtcacc
      961 gcgccgttgg cggaggggtc gagcaggata tcaacggcgg ctgcggcggc gacagccacg
     1021 aacctgtgcg cgaaccgaaa cgcccggccc tgccagactg catgaagcac gtcgctcccg
     1081 acgatgtgcc gcccctcgtc gtcgatggtc cccgtgacgc cgtagcggtt acttggagtg
     1141 aagaccatac atgcctccaa aaagggagag gatcagctct ccgtcatgtg gccgcgcatc
     1201 acgccgaggt tgaggctgcc ggaggtctgc cggaggttgg agaacggagt ccgccagata
     1261 tcgtactggt agacctcagc gccgagtctg cggtcgtatt cgcccgttgc cttgacctgc
     1321 gagaggacgt tgatcacgcc cgggacgcgg ttgcagaccg cgaacacgaa ccggttgccg
     1381 gaggtgtcat cgaggttgtc gatgtccgtc gaggagtaga tcgggacgtt ctgcgtcggg
     1441 tgcctcatag cggcgttgcc ccactcgctg atgccctgca ccgccgcctt gtcgccgacc
     1501 gcgtcggaat agtaggctgc cgcgagggtg cccatgacgt aggcggtcgg cttgtagatg
     1561 cccatgccga tcgagagttt gttgagcgcg agcgtgggct tggcgctcgt ccagtcgccg
     1621 aggtcgccgt cggtcgagta gatctgcggg gtcgtgttga gccgtgccgc gatcagtttg
     1681 ttgaggttcg atgcgagggc tccggcggcc tgctgctgct ggagactcat cgggtcgccg
     1741 acgtcgctct ggatcttcgc ctcgtcggag accgcgagga ccacgcgatc cttgagcacc
     1801 tcgatgtcgt atccggccgg ggtgccggtg cctgccaggg cttcctcaaa ctctgcgagc
     1861 tgcgaggaga ccgggacgtt gccgagcatc gggatggtcg ccttgagttt cgccgaggtc
     1921 agcgtcttgc agatgggggg cgctgcgaga tcagtgagtt ccagcgcttc catgatgacg
     1981 ggcagaatga ggcgcttctg tgcccacgtt ccatcgatgt gcagttctcc gagtatacca
     2041 gtatcggcca tgttagatca cctgcccgag gaagacgacg ccgacagtcg tgtctgccgc
     2101 tcccacgatc atcttgccga gcgggcacgg tcccgctgcg ggggcgagcg tctcaaacgg
     2161 ctttgcgacc acgattgcct tgccggtctt gccgccgacg atggcgccgc gataacagtt
     2221 tccatctgcg gtcacggcga cagggccgta gacgacggcg cgaccgttac cggacgagtc
     2281 aatggcctcg atcgcgaccg catacggggc gttgcccgac gcatcgcact tcgcggcacg
     2341 gggaagggtc gaagtcggtg tgagcgtcag aacctcaccc ttaacgattg ctgcacccca
     2401 cgcggtgacc tgctcgatac gggcaccggg gaaaaactcg cctgctgtgt atgccattta
     2461 gaccaccttg attccggtgc gcttggcgaa ctctgctgcc agattcgcga actgctgctc
     2521 ttcggtactc tcggcaccgt cggcgctctg cccgacgccg gagccctctg cggccttctc
     2581 gccaggctgc gtgttcttga acgcgaccac cttcagcgcg aacgcgccgg gggcggcctc
     2641 gtactccttg cgggtctcgg cttccttcgc accgagccag cccgccggga ggttcgcctt
     2701 catgacgtcc cactcggcgt ctttcttcgc ggtctcctga gcggccttga gcgtcgcgag
     2761 ttcctgcttg aggttctcga actcagtcgt cttcgttgca agttcctgct tgagattagt
     2821 catctctgcg aggttgtcgt gctgcgtcgc cggtgcccgg gttaaggcgt cggcgattgt
     2881 cttgagcagc cccttggatt cgtcgtccat gtctgtctcc tgtaggttgt ggaatgttgc
     2941 tccgttgtcg ttcgggaagc aattcgggca cgctccgcgc tcgaacacca gaacgtgatt
     3001 cggggtgacg ttgcctgcga tccgcgttgc accgggccgc ccggggtccg gggcttctgg
     3061 agaagagaac ccggtcgaga gcgagagttt tccggcggcg gcaagggcct cgatctccgg
     3121 atcggagaac gcgaccgccc cccgtagcgc aggctcgccg ctctgcggga ggttggcgcg
     3181 ggagacgctg ccgacggggc ggaactgcgg cgggagggtg ccgtttgcga tatcctccgg
     3241 tcgcgggtgc tgtgccgggg tgccgggctc ggtcgtgacg tagatcaccg gaacggtgtt
     3301 ccacaggtcg accgagggcg cgaagtgcgc ggaatcgtag aaaacagggt gcccgttgtt
     3361 cgtctggtag acgtcgaggc gctggagcgt gacgtcgtgg aggttagcga aaacgttgga
     3421 gtgcgggata tctccacgag tcatactata tgcttggcgc ccatacggca tatataaaat
     3481 tgtgggtctg aaaagggggt attggtggga tttgcgaggg ccgctcacgg attcggggca
     3541 gcggtgccgc gcaaatcttt acccttttcg aggtagtcca cgaaccccgt gaccccgccg
     3601 tgtccgtgga accgggccgg gagcccatag aggttgatgt ggcgcttgat ctgcgcggcg
     3661 aggtcggctt ccccggccgg tgcgatcatg cgcttgatcc gctcctggta gtgccggatc
     3721 gtgtcgcggg agatgtcgtc gccgtcgaac ccaggaaggg tcttgatctg tgccggaaac
     3781 atatcgaggt gctcccggac gatcgccatc tgtcggtcgt tgaggtctga ttttcgggtc
     3841 atgctgctaa ttcctctgcg ctcacgatga tcgcgccgtc gccctcgacc ggcatcccgc
     3901 gctcctcgac ggcgaaatcc gcgtcgatga gagagcaccg gcagttgggc tcgccgaggt
     3961 ccggagcgtc ctggatcttg tagatcttgc cgtcccggga tgcgtggtca ggacgcacct
     4021 cgccgtcgcc agcggtgaca tactgcacgt agtggacccc tgccttcgcg taagtggcga
     4081 accgggcatc cgtccttatc ttctgcgcct cggtcctcgc ggcagtcagg gcgttgtgct
     4141 gcgtgccgtc gaagtagtcg cggagacccc gggcgatctg cttggggtgc atcccgcccc
     4201 gctgcccctc cccgatgata cggaggatct cttcctggtc gcgggtcgcc atgtccgaga
     4261 gccagggttt aaagacgcgc tgtgtcgata ccgagacctt gccgttgccg agatccttga
     4321 caacctgctc gatgatatcg gtcccgccct tcgccacctg cgcccggtat gcggcgacct
     4381 gctgctcagt gataccctcg acgacggagc gggtccactt ggttcgatgc atccgttcca
     4441 actcgcggag cgtggtggtg gctgcggctg ctgcgacggc ctcggcctcc tcgaccagag
     4501 cggcggcgat cgcctcctgc cggtccgcga ggatctcctc gatgagtttc tgctgcttgt
     4561 cggtcggcat ctagcctctc gacaggatgc gctcgatcgc ctgnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagaggt tgcgccggat
     4741 ctcttcgggg aggatggact tcccggcgat cgcggtctgg atctgttggg cgacgacttc
     4801 ggatcggtcg agttcggggc gcttgagttg gatccggaca tacgtatcat cgtagccgtt
     4861 cgcggtcagg agtggctgca agaacccttc gtattgctcc tcgatccacg cctgcgtccc
     4921 gccgataaag ttgttccaga tcctcatggc tccagaatcg gtgccgccga tcgtggtgcc
     4981 ctcactcttg aggacggtgg tcgggttaaa atacgcctcc aaccagtgcg tgagctgctc
     5041 cagtcggtcc cttgcggtct ggctctcgcg gatcttgaca tcagggaagg tgaggccgtc
     5101 ggggaggaag aacccggtat ctttgcccca cttcttgacg aaattatcgc cccaagtttt
     5161 catatccggc gtcatcgggc cggtgacctg ggggaagatg agcggagcgc cgacgcggtg
     5221 cacctgctga tcggcggcgt ggttggcgtg gtcgatggcg gcgatcaccg ggtannnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcct gccggaacga
     5401 gatcgccggg agatcgcgga tctcatcgag ttcgtagcgg ccgttgcggt tgcggtagcc
     5461 cgggctcttg acggagcagc cgaagcccat gcactcggtc caggcgatct tcatcgaggg
     5521 gtagacctgc gcccgggcgg cctgctgcgc gatccacgcg gagagttcgt cgaccttctc
     5581 gccgtctgcg tcctcgacgt agatcgccgg ttcgcccggg aagacctgca cctgcatgtt
     5641 gatgacctgg ttgcggaggt ggatgtttcc ggtcagatat ttccaggcca tttcgggcgt
     5701 cagtttcggt gcggtgtagg cgttttccga ggactggaac gatacctcgc cttcggcctt
     5761 tgtgccccgc atgacgcggt tctcttttgc tgtgatctct ttctctgcca tgttagtgtc
     5821 ctgtgatgat gcaggcgatc gtgnnccggg aagacctgca cctgcatgtt cacgatctgg
     5881 tttctgaggt ggatgttccc cgtgaggtat ttccacgcct tctccggcgt caggtccggg
     5941 gcagtgtagg cattctccga tgactggaac gcgacctcgc cctctgctct cgtgccccgt
     6001 gagatacggg tctcttttgc ggtgatttct gcttctgcca tttatcctcc gatgtatgca
     6061 acgatagtca taaacacgaa cagcgccgcg aacgcgagcg caaaccacgc ggcgaggctc
     6121 atctgctnng gtgctgcgtc gcgcccggag gagagttcgg tgatcgccca cacgagggcg
     6181 tccatgcggt caggggactc gtcgccgggc acccactcgc acatctggtc ttcgaggtcg
     6241 gggagtgcgc cgacgtggtg cacctcgccc ttctcgtaga gtgcggcgac cg
//
LOCUS       DAJB01000157            9742 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_95579, whole genome shotgun sequence.
ACCESSION   DAJB01000157 DAJB01000000
VERSION     DAJB01000157.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9742)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9742
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_95579"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1906 a         3034 c         3052 g         1750 t
ORIGIN      
        1 ccccgatata gcctcttgtc gcacgccccc ttatcgctcc cagcgtggga ggaggggcag
       61 aagaagatgg ccggaggcga gccggtcgag gggctcgagg tagagttccg ccgcaaagac
      121 gggtccgtgg cattcgtaga actgaacgag tccccgatca tcgagcatgg aagagtggtc
      181 ggcgtccagg cggtgggcag ggatatcacc gggcgaaagc acgacgagga gctcaaaaac
      241 cgggccttcg ggcagatcga acgaaacatc gaacagtttg ccgtcctcgg cgaccacatc
      301 cgccagcccc tgcaggtgac cctggggaga gcggaactgc tcgacgacga gaaggcggca
      361 gcgatcattc gtaatcaggt cgagcggatc aacgagtaca tcagacagct ggaccgagga
      421 tgggtggagt cccggatggt ccgcgatttc ctccggcggc atgagcgggg gtgagcccgg
      481 tgattcagcc gaaccggccc ggcagggatc ccccgcggat tgcttaccct taccgcccgg
      541 caggtggttt ttccgtgacc gaatcgatcc cgatgtcaca gtcctgtatc gcgccgtgtt
      601 cggaaaaaac cgagcgaatt tctccaagaa tccgctctgc cagcattcgc gcatcgccct
      661 cctggtcggt atccatctcg atgaacccgg ccagttctat acggaacttt ttcatggcat
      721 ttacctccgt cttcccgcag agcctacccc tggtgtccta aagtccatgg gtagttggct
      781 cctctccgat actgctgaga accgcctgca cgaccctgtc cacatgctcc cacgtggggt
      841 tatccgcgct catacccgga cgccggacaa taaacgggcg cccttcgtca ccggctttgc
      901 gcatgtcggg gtccagaggg atactgccga ggtacggcac gccaaggtcc tctgcggcct
      961 tcttcccccc gccctgtccg aagaggtcga tctccttgcc gcagtgcggg caaaccattc
     1021 cactcatgtt ctcgacgatg ccgagaacct taatatccat tttctcgacg aacttcacgg
     1081 ccttggtcga gtcgagtatg gcaacctcct gcggcgtcgt gacaataacg gcaccggcaa
     1141 cgttcggggc gagctggatg atcgataacg cctcgtctcc cgtgcccggg ggcagatcca
     1201 ccacgaggta gtccaggtct ccccagttga catctgcgag gaactgtttg atgacctgca
     1261 tcttcatggg gccgcgccag atgacaggag tgctcttatc cggcaggaga aacgccatcg
     1321 aaacgacgcc aagtgacggc actacgtata ccggttcaat cgtcgtgccg ttcatcgacg
     1381 tgagtttggt ctcctcgatg cccatcattt tggggatgtt ggggccgtgg atatcgagat
     1441 cgagaagacc cgtctggtag ccgtggttcg caagcgccat tgcaaggttc gcagccaccg
     1501 ttgtcttgcc gacgccccct ttcccactca gcaccaggat aacgtgcctg acactcactt
     1561 cgactttttc aaacgggttt ttctgttgct gctcgatttc aggagaacca ccgctctggg
     1621 tctccgcatt catgaccgta tcgtttgctt cttccataac atatcctctg taccccatgc
     1681 gtccggcgaa gaatgacttt gttcgctgat ccggggtgtt tataagccga gttcacaccg
     1741 ctggacgatg ttgttcttcc cgccaccctg gcatcctcca gacgaaaccg gcattccggg
     1801 tttgcctgga gagaggaaga cctgcgtatc aaccgccaga cggctaatgc cgcaacacct
     1861 gtgtgctaca cggcttacat aacaaagtta acgccgttaa cagaaaacat tactgatact
     1921 tacatcctca agaaccccac ccgaagatcc tctgcaggga aaaagtctcc ctgcggcctg
     1981 atcgggcacg atcgaggaga ctgagacggg tgcagaagac catcttgccg ggaaacccac
     2041 gttaaagaga aggtatggac acagggtaga tagagggctg tgtgatggca tttgatctat
     2101 cgaaatgtga cttctcgcgt tgcagtggag aatgtctcgt ccgctgcccg tatgtggcat
     2161 acgacgaggc cgacgcaaag agcgccataa aatccctgat acggggagag gagcacccaa
     2221 tcctgaaaga gtgcatcacc tgcgcggcat gcaacgattt ctgtccacgg ggcgccaatc
     2281 cctgggacct cattgcacgg cggcaggaag agacaggcgc tctcggcatc cccccggatg
     2341 caaagccggc gagcgactgg ctgacaaagc cggcgacggt catccggagg gggaagcagg
     2401 gcggcccgct gatctccgcc ggcggcatct acgaggtggt gccccaggcc gagttcctca
     2461 cggggcaggt gtttgaggat gcaaccctca tcgggggagg gccgtacgcg tgcgggttca
     2521 ccgagaccca cctcgggcgg gcgtcccggc cgctgaagtc cttcccgcag ttcatcgcga
     2581 acctcgcaaa agcggccgag gagttcgggg tcgatgagat catcttcacc cacgacgcct
     2641 gctacaatgt ggcgacgaca ctcgcgatgc aacaggggat agaagtgccg ttccggccgg
     2701 tccatatcct cgagtacatc aggaactggc tccgcgacca ccctgaccgc attgtccacc
     2761 cgctcggcat cagcatcgcg gtgcagggcg gctgcacaac ccgctacgcc ccgaaaggcg
     2821 gggaccgcga gatctggtcg gactggctcg ccgatatctt cgagctgatc ggtgtcagat
     2881 cggtcgagga gaagcggacc tacacgggag acgatcggct ctgctgcggg tgcgggatct
     2941 tccataccca gcacgaacgg gcgctcgaga tccagcgcag gaatatcggc gacgccgccg
     3001 aggccggcgc cgaagagctc gtcttcatct gcccggcctg catctccgtg atgcgcgcga
     3061 cctgccggaa gatggaactc gagccgatct acatcaccca gctggtgaaa cgtgcgctgg
     3121 gtgaggaact cggacccgcc ggcaccgctg cgttcgggta cccggtgaag taatcaggtg
     3181 aggtgagaag aatggtgaag tttacgaagg agatgaaaga agccctgcgg gttcccggca
     3241 aaggagggag cctgatctac ctctgcacgt cgagcaggga cggcaacccc aacatcgccg
     3301 cgatgcggtt cgtcgccacg cacatggacg acaagatcct gatcgccgat atgttcctct
     3361 tgaagacgaa ggcgaacatc aaggagaatc ccaatgtcgc ggtcaccatc tgccatccac
     3421 tcgaagaagg gcggtggtgg gtcttccggg ggaaggccgt ggacatggag tacgggttcc
     3481 cgcgggattt cgactggtac ggcgcaaacg cgactgatat cctggacgag tggggcaact
     3541 ggaacaagtc cgaaccgccg gacgaggtgc cgcccgacat cgtcttcccg agagcagtcc
     3601 agcgcggggt tgtggtggtg catgtcgagg aggcctactc cattgagccc gggcatacgg
     3661 gcgagaggat tctttcggag tgatcgggat gacggtaaac cttacagaca ggatgaaagc
     3721 atggattgag gcgatgggat gccacctctg tgtcgcgaca ccggggggcg tgccgttcgt
     3781 ggtgctgggg cgctacgcgc aggcgacagc cggcgacgag gtggcgttcg cgctcgcgga
     3841 ggacgaggcg aaggtcatcg cccccgtgtt gtcggagaac ccccgggtgg cattcggcat
     3901 atcacggcag ggcggcatcc gcgcggcata ccagttcaag gggacgggac aactcctccg
     3961 ttcaggggag gccttcgacg cggttgcagc gcgagcaaaa gacgagaaag ggatagaaac
     4021 cggggctgta ctcctcgtgc acctgaacga actctactgc acgaagcccg ggtatgaagc
     4081 cgggctgagg ctggatggca tgccacctga ggaactcgat gcgtgggagc ggcagcgctg
     4141 gaaggacgtg ccgaggaagc gggcctgagg ttcgatgacc ggcgaccgaa aagagacgga
     4201 cggcaagaag atgccggacc ggttcccctg gatcattccc ttcgcatgcg aggggtgcgg
     4261 cgactgtgtc gaggcgtgtc cgaccggctc gatcgaactt gtggacctgg gaaaaaagat
     4321 cgacaaggcc tggatcacga accccgacac ctgcatcggg tgcgggaagt gcgcacaggc
     4381 ctgtgtcgtg ggcgccgtgc agatgacgtc gtacgtcgag aaggcactcc ggcgataccg
     4441 cgagaagacg acgccggagt gaccggcgat tcggctccct cttttcagga tcgtggggcg
     4501 aggagccccc cgacttaagt cgggggaggt atcggccctc ggcgtgtccc gatacacttt
     4561 caccccgcac gggcacttgt tatgcctggg gagtgcccac agggatctgg ttgcgccaca
     4621 gaaggcacga agcaggtgag tatctttcct gcggattacc gttacacgcg attggcgcaa
     4681 acgacaccac agtgcagcga cattgctgcc agggccgccg tcaaccggcc cattgcggtc
     4741 tgctcccccg ctattggagg agaagttgaa tcgcaagcct gtaaagagcg tggtaccaca
     4801 cacggcacca tgcccccgac ttcagtcggg ggtagttgac cgcaccccga atgattcgag
     4861 cagcacgtcg gcgttcaggc tgccgaaatg gtagacctcg ccggacgttg aatcgttgat
     4921 cgtgacctcc gccggcgaga aggcgagcag ggagtccacc ttcaggaagt cgtagccagc
     4981 gtccttgagc acccggccaa acggcctccc gtagtcaggg gaggtctgcg agggcagggt
     5041 tgcaaagacc gggtcgtagc cttccgcgac gagggagacc gagccgtagt agatgttgca
     5101 gtcgttggtc gcccccatcg cctcgatgcc ggttcgcttt acaggcgcta tcggcgaccg
     5161 ccccgccgca tggctgatgc ggagggtgtc gtatcccctc tcttcgagtt tgtggagggt
     5221 cgccgtgacg acccgcccgg agatctgtac cgagccaacg aggctgcgtg tcggggcgac
     5281 aaggacgtag aggttttccg ggtcgatgcc gcatttttcc gcgatgaaac cggtgacctc
     5341 ctcatccggg agggtatccg cttccagcgc caggatgccg aaccgggagg tgtcgtgata
     5401 gccgatcttc cgataggtct ctttcggttt ggcggccagt gcccgcgccg ggcccgaagc
     5461 catggcggag tagttcccga ctttgagcac ccatccggcc ttctgcgaac cgagacacgc
     5521 aagagcggga tggctcacga cgaggttcag gaacgggacc ggcacgtccc ccacccggtc
     5581 catggttatc gccgtcgtcg caagccctcc aaggcaggcc tccacaaaga gggtgcccgc
     5641 ccggtaactc ccgggcacgt gcacaccgca gtcgatgcac gtcacaccat tctcctgctc
     5701 tgcaaccgct accctgagtt cgtcccgacg gtcgatcatc tctctcacgg caggcatcgc
     5761 cgtgctgttg atgctgagca tcgtcttcac ctctcttacg ggggatagga tacggaacgc
     5821 tttagcagtt ctcctccggg catacacgag aaattctctc acgaccccgg tctggtgccg
     5881 gccgcgccga gcagcccggc atcggcgagc cacccgatca gaccgagggc aatgccgccg
     5941 atgacgatcc cggcggctgt cgctcccgcc acccggtcgg ccgcacatct tcggcaaact
     6001 gccgggcggc ctccggcgag gggaactcga aggtccgttc catgggttca ccccgacacc
     6061 tcccggccat ggccgtctgc acttcgccgg gaagtaggtt gcgcgaccct gtgcggggat
     6121 ggtataaaaa ggtgagactc gtagggtggt tcccggagac cgtgccctca ggtaggttcc
     6181 atagaatttt gcgttgtctg ccagggtgcg ccgcaccagc tcattagtgt cccgaagtcc
     6241 tgatagatcc catctttgcc tggatcgaag agaggggcgg agttcatctg caatgccggg
     6301 gtctcgatcc cacacccttt cgcgccgttg cagtgctcgc acatcgtctg gtaattatca
     6361 atggaggagg ggccgccttt gcacttcggg actatgtgat ccttcgtaaa catcacttct
     6421 tttccatcct tgagcccgta gaggttcagg cgatactcat cagccttgca aagccgttcc
     6481 agagcgaagt atcgagcctc gaggccacaa cagatacaga ccgttcccga atgcaggaaa
     6541 cagcgcatcc ggtccgaggc catgttgacc aggacgcccc cgaaccggat acggtgtcga
     6601 ttcccgattc gccccgatac gccgaggaca aagggcatga cgtaaccgac cgagtacgta
     6661 gcaagccgat ctctcccacg acgcggtttt tttctccaat tacgggtcat cgccgccatc
     6721 cccgaatgcc gcgaatccga tctccgccgt gccgtagagg gcaagaacca gccgatgcag
     6781 gatgtccagg gcgtcatcag cctcaatctt cccgggacag gtccgggtcg gatacgggca
     6841 tctcctgatg ggagatccat cctcgcagag acaggagtgg cgaaacatga tgacaagtac
     6901 acggtcatca atcctgactt ccaggttctc aggctcatcg agccacacgc agaactcgca
     6961 acagtccatg tactccgctc ctactgcttc tcaatggcga actcgcccgt ccggatcttt
     7021 cgcatacgac cggtgcacgc gccgtaatgt ccccggccat acgggtgccg aaagttccct
     7081 gccctataat gggcagggtt cctgatcacc ccggagagaa aacgcaccag cataccggga
     7141 tacccgggca gcacctcgag catgtttgac cggacttcag ttccctgttc tatcgctgcc
     7201 atgtctatgt acctgccaga attctgtcaa gagtccggtt gcagagccgg tcggcgatct
     7261 gcacaccggg ttccgtccgt cggacgtggc agaaacgaac tctttcgcgg ccgatctgct
     7321 gcaccaggtc ggcaataagc ccgcgaagcc gcagatgagt acgacacgtg cacacgctct
     7381 cgccggtgat ctgccggatt gccaccaggc tgtcactgta cagggcaaca ttcttcccgg
     7441 ggtcgtgctc caccagggag cggagtgcgt gaatgatgcc ccagtactcg gcctcgttgt
     7501 tggtcgcgac gcctatatag ccggaatcct gcgtcacacg tgagaacgcg tcaaaagagt
     7561 cgaatctgac atacgcccac gcggaatcgc ccgggttgcc tcggcaagcc ccgtcagtat
     7621 aaacccgcga gtacatacga aactattgtt tatccaggga aaatatatag ttgatcattc
     7681 ctcgcctcgc catctcctga catacgggcg ccagagatcc gcaggtaccg cggccgctca
     7741 tgcccaaaca gcgcaatcag ggaatgttcc gaaaaacagg ggccctggaa cccacaaaga
     7801 gcagtgccgg cggcacacgc agcgggacgc cgggcagcgg ctggttttat gacggtgcca
     7861 tcaaaataga taacggtagt tatgatggaa aaaaccccgg caaacttcat ggacggcgcg
     7921 atccccgccg gtctcctcgc cgccgtgcgg gaacagcggc cgctggtgca ccacatcacg
     7981 aacagcgtca cgatcaacga ctgcgcaaat atcacgatct gtgccggggc cgccccggtg
     8041 atggccgagg ctcccgagga ggtcgccgcg atggtcgccg ccgccggcgc ccttgtcctg
     8101 aacatcggga cgctttcgcg aggccaggtc gaggcgatgc tgatttccgg ccgccgggca
     8161 aacgagctcg gcatcccggt catcctcgac ccggtcggtg cgggggcgac ggagttccgg
     8221 accgcgaccg tccggcgtct cctcgacagc ctcgacatcg ccatcctgaa ggggaacgcc
     8281 ggggagatcg gtgtcctcgc cggcaccggc ggaagcgtcc ggggggtgga ctccggcggg
     8341 gtcgcgggcg atcctgtcga gacggcccgg gaatgtgccc gggcgaccgg caccgtggtg
     8401 gcgatgaccg gggcggtcga tgtcgtcgcc gacgcgcgaa gggtctttct ggtcaggaac
     8461 gggaatccgg cgatggaccg cctctccggg accgggtgca tggccgcttc ggtcacggcg
     8521 gcgttcgccg cggtcgccga cgactacgcg gttgcatcgg cggcagcact cgcggcgttc
     8581 ggccgggcag gggagcgggc cgcggccggg ggcgtcgccg ggccctacac gtttcggacg
     8641 gcgctcttcg acgaactcgc cgggctcacc cccgacgacc ttgtcgtgca cgcgcggatc
     8701 gaggagatct atggcgtatg acctctacgt gatcaccgac gaggggctgg gccgcggcct
     8761 ctcccacgcg gagcttgccc gccgggccgt cgccggcggt gcggacgtag tccagctccg
     8821 cgacaagagg ctctccggcc gggacctcct cgccgcggcc tccgccatcc gcgaggtcac
     8881 ctatgatgcg ggagcgctct tcatcgtgaa cgaccgcctg gacgtggccc tcgcggccga
     8941 tgccgacggc attcacctcg gcgagagcga cctccctatc gaacacgccc ggaggattgc
     9001 gccgccgggg ttcatcatcg gggcctcggt cgggtccgtg accgcggcgg tccgggccgg
     9061 gatagagggc gccgactacg tggcgctcag cccgacgttc tcgaccgcat cgaaggacga
     9121 cgcaggggag ggccacgggc ttgccatgct ctcggcggtc cgtgccgcag tctccctccc
     9181 cctcgtcgcg atcggcggca taacccccga taatgtcggc gatgtcatcg ccgcaggggc
     9241 ggacggcgtt gcggtcatct cggcggtcgt cgggcaggac gatgtcacgg ccgccgcacg
     9301 ggatctccgg agccggatcg ccgccgcgaa ggcgggaaag cgttaatcca cccctccggc
     9361 tcccggggca gcaggcgtcc gctggtggaa ggccgcccgc caccggccgt cgctcctggc
     9421 atacacggtc gatacaaaga gtgtctgccc gccgagggag acggtatacg cgaggacggc
     9481 acccgcctcc ccgaggggca cgaccttcgg gtcagcgatg gtgtacgccg gcagttcgtg
     9541 ggggttctgc acgatgtccc ggaggatccc ttccctgtcg aggacgcccg gagggctgac
     9601 gacaagggct tccggcgcga ggtactcccg atagaactcc acgtcccggc ggtcaacggc
     9661 ctcccaggcc cgcttctcaa tatccagcag aacggttaag aggtctttca taaggagcac
     9721 gtcaactacc cccggttaaa ac
//
LOCUS       DAJB01000158            4094 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_151692, whole genome shotgun sequence.
ACCESSION   DAJB01000158 DAJB01000000
VERSION     DAJB01000158.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_151692"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1040 a          995 c         1266 g          793 t
ORIGIN      
        1 taattagtta agacgttatg tatagcatgg ggtacgccga catctatgac gatatccgtt
       61 tgagtggagt caaagcagtc aaggaccgga acgtctacgt gattcctgcg gggatggggc
      121 cttggctggc cacagctgaa cagccgttgg ccatgcaatg ggcagcaaaa ttgttttacc
      181 cggacagatt tggtgatgtg gacatagaaa gagagatgca gtggttctat acgacgtttt
      241 acgactacga cctgtcggac caagagatat cggagatgct cgcagggggc aaagttgtcg
      301 atcctgggac acggagggat ccccactgat ccgggcgcta acgggtacgc ccggaggaga
      361 cggggtcgct agtcgataac cagggcgata cagggagcga gtgccagatg gtatggccgg
      421 gatagagaga gagggcagtt aaagtccccc aggggagtaa caggcgatct ggtgagaggc
      481 tggaaaggaa gagaaaaagg gtacaagtat caaaggatgt gaaaaatgac acaggataat
      541 aatgtgaaat gctggatcga ctgctggaat gcatcagcac agaacagcag gataatcact
      601 gacgacctgc aggcggccca atgggacgag cgagctgacc gctttgtaca gaacatggat
      661 gaggaaaggc accggaaacg agttgccgat gtttttgcaa ttctggagga ggccggtttc
      721 tcacccgagg gcgcacgtgt cctcgacatc gggtgcggac cgggcaccct ctccctcccc
      781 cttgcccggg ccggggcgga cgtcacggcc ctcgacatct cgacgaggat gctcgaccac
      841 ctcaggaaaa ccgcgcaaaa cgaggggtta cgtataaacg cggtcgaatg ctcgtggtgg
      901 tcggccgata tcgacaaact tgggttccgg gagaagttcg atctcgtgat cgcgtcgatg
      961 acgccgggaa tcagggacgt cgagaccttc gaccggatga tggcgtgttc aaggcagttc
     1021 tgctattaca gccgcttcgt cgggagggtg ggcacagacg gggctcgccg ggagattttc
     1081 aggaaaatcc tgggtgagga acccgacaac cccgggccgg atatgctgta tccgtttatg
     1141 tacctctacg ttcttggtta ccggccgctt gtgagactca gccatgtttc aaggacccag
     1201 gagcgggact gggccgaagc agcggaagag acgatcaacg atctgaaatg cacccgcgat
     1261 atttccagcg atgtcgagga aaagatcagg gagtactaca aaaatgcgtc gccggacggc
     1321 aaatatcgtt ccgaaacaga gagttgcagc ggcatgatgg tctggtctgt cacggccgga
     1381 gatggggtat aagcgggctc aaccgttcgg gtctcttcag ttcgacatgg gcggggaaat
     1441 taacggagaa ctcctcgcat accttctccc ctccctgtcg ccgaacttcg ggggtttacc
     1501 ggagtataac ccatcagaga tctcccggcc tggttccgta ccagagggtg ccccggcgaa
     1561 ctcaccgacc tcgcaacacg ggacaatcaa ccataaattg ttaaaaagtc tattttataa
     1621 atagaaagca ttaactcccg gtgttgaaga cgttatatca cacctgtgag gcgatatgcc
     1681 gggacacctg aagggctacc cgtacgggac ccgcgtatga ggcctgcagg gggtgttgat
     1741 gatgaaagag acgagtacag tcctgaaagc gttcataacc ctatcactca tagcacttct
     1801 ggcgttctcc tgcggctgca ccgggcaggg acaggcgacc gacagcggga caaaggtgat
     1861 cacggacatg gcgggacggg aggttgtcat cccgaagaat atcgacacca tcgtgacgga
     1921 tggtgccgtg ccggtgctga acagtttcat gttcgtcatg ggacaggggg acaagatcgc
     1981 taataacctg ccggactcat ttatcaaaca aggcaagtgg aagtaccagt atgtgatcga
     2041 gcccagtcta aagaataaac caacgatcca gaccatgtcc ggggacttca acctcgagga
     2101 actcatgaag atcaacccgg atgttgcatt caccatgagc atgtcctcgg tcgataaact
     2161 cgagaagaac ggtatcccag tcgtttatct ctcctggacg gatgctgagg acgtgaaaga
     2221 cacgctgctg ctgctcggag aggtcttcgg cgagcaggag aaggccgaga gatacgtcag
     2281 gtacttcgac gaggccaccg cccgcccggg taaggtggtc gcatctatgc cggacgacca
     2341 gcggccgaag gtgttgtttt gttcatacaa gggtatgtct gtgaaccaca agatagccga
     2401 gtggtatatc gagaaagcgg gtgggatagc tgtcgctaag gaggcccatg ttgcggagag
     2461 cctgaagttt gatctggagc agttacttgc ctgggatccc gacgtgctca ttcttagcag
     2521 tcatgatgag aaccccctgg tttacaacga tacgaggctc agcggcgtaa gtgcagtaaa
     2581 gaacgggaag gtgtatgcgt cgcccatggg agcacacctc tggtgccatc gcacgagcga
     2641 gcagccgctg atggtgctct ggacggcgaa gacgatctac ccggagcagt tcaaggactt
     2701 cgatatggag gcagaagtca tgaccttcta taaagagttc tacaactgcg agataacgcg
     2761 agagcaggcg gcggagatac tcagcggaac ggcagcatga gccctgcata atcaaagagc
     2821 cggcatgcgc ggggagttaa cggagaacca gtctctcccc cgccctgttg ccggaccccg
     2881 gatattgcgg cgatcacgga atttgttgtc gcacgtcagg aatatgcaaa gagccaattg
     2941 tacaggaatc ggtgtgtgtg gtagttacag aggaggaagg gatcagagat gaaaggaaaa
     3001 actacaggct ggaaagcgtt catagcccta tcactcatag cacttctggc gttctcctgc
     3061 ggctgcaccg ggcagggaca ggcgaccgac agcgggacaa aggtgatcac ggacatggcg
     3121 ggacgggagg ttgtcatccc gaagaatatc gacaccatcg tgacggatgg tgccgtgccg
     3181 gtgctgaaca gtttcatgtt cgtcatggga cagggggaca agatcgccta tagcacgcct
     3241 ggattatcta agtaccagtc tgtggtctcg cccggtttaa gggacaaacc gaagatccag
     3301 gggggcatcg agctcgagga actcgtgaag atcaaccccg acattgtgtt caccgcgagc
     3361 atgcctaaag tagaagcatt cgaggaacgg ggtctcactg ttgtttacct gcccatgccc
     3421 gagacggatg cggagctcgt gaaaaatgtg gtaaggatac tcggagaggt cttcgatgag
     3481 caggagaggg gcgagaggta catcaggtac ttcgatgaga ccgtcgcccg cccgggcaag
     3541 gtcgtcgcat ccataccgga cgaccagcgg ccgaaggtgc tgtattgcag ctacaagacc
     3601 atgaccgccc accacaggtg gtggatcgag aaagccggcg gtatatccgt ctctaagaac
     3661 aatgtcgcga agaggataac attcgatctg gagaagttaa tcgcgtggga tcccgatgtg
     3721 atcttcctcg gcggtcaaga gatcgatctg gtttacaacg acacgaggct cagcgatgta
     3781 agtgcggtaa agaaccggaa cgtatacgca atacccggcg gaacggggcc ctggggctac
     3841 tcgagcgagc agccgctgat ggtgctcttg gcagcgaaga cgctctaccc ggagcagttt
     3901 aaggatctcg atatggaggc agaggtcata gccttctaca aagagttcta caactgcgac
     3961 ataacacggg agcaggcggc ggagatactc agcggaacgg tataagcacc gccgaatgct
     4021 ccgtgcctct aaattcgcat agggggagtt gttgcgggtg aaagaaaaac tacggtctga
     4081 aaagcgctca taac
//
LOCUS       DAJB01000159            5171 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_79733, whole genome shotgun sequence.
ACCESSION   DAJB01000159 DAJB01000000
VERSION     DAJB01000159.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_79733"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1014 a         1756 c         1564 g          835 t
ORIGIN      
        1 ccccgccccg gggggcgggg gcagtcatgg cgatacccaa tggaaagccg tgccccggat
       61 tgcaatcaat cgggaatacc cggacgggat cgcaacaacg ttatgatcag ttgtttcgca
      121 gggcactacc cgccacgggc tacgatgggg acaaaaccat caccgggcgt tgtagccggc
      181 agagatcccc tcgcggcctt ccaggtcgtc gtaggcggcc actttgtacc ctgcataccc
      241 gcccccgagg ttcatctgca ggaggacgaa gatccggagg gcgggatgca cccagatcgc
      301 gacatcggca tcgtcctgct ccggtatgaa gtttaacgac tggagtgctg ccctgacatc
      361 ggcatagttt ggtgtcgact ggatcatctc ctcgatctcc accatcacgg cccgctgctg
      421 caggatctcg atatcctcaa gcatgccatc ccgatcgatc ccctcgtata aaagatgatc
      481 cggggccgga tccggagaag aaccacgcat tctgcggcgc cgtcgccacg tggaagcccg
      541 gggattatgg tcggtatgcc cctgccggga tacaggatcg cccgatgaag tgcacaacct
      601 tcatcagaca cctcgtgaaa acatacagca aagaaggccg aacggaggac ctatgaccaa
      661 cgacgatgac tcaaggacac tgatcctcga tatagacacc gacgatctgc aggagctctc
      721 cgaatacctg gacgtgctga gcagcagcac gcgcttgaag atcctgaagg tcatcgaacg
      781 aagtccaaaa gatgtccggc agatctcggc cgcgatcgag acgagttacg agaacacaaa
      841 gaagcacctc gacaaactgc tgagcatcgg ggtcgtccgc aaagaggccg gtctctcccg
      901 cccgaccgca aaaggtattc acccggtctg gaaatactcg ctcgtgcccg gggggctcga
      961 ggcgatcacg agaagcctcg ggctcttctc gaacctgaaa ctgacgctca cggatgctgt
     1021 gctcgcgaag agacttgccg gtgtccggga gacgttctcg ggagagttca ccgggcaact
     1081 cccggtcgtc atcctcctcg gcgggtcgga ggacggcagg gtcttcccgc tcgggcatga
     1141 caacatcgcc ataggccggg cggacccgga cgcacccggt cgagccgggc aggatatcgt
     1201 cctcggcgaa gagtacgcgg cggtcacccg catatcgaga ccgcacgccc ggctcacccg
     1261 ccgccgggac ggtgcctggc tcatcgagga ctgcggcagc acgggcggca ccttcgtgaa
     1321 cggtacgccg ctcgaccggg gcacgcggcg ggagctccac gacggggacc tcgtcgaact
     1381 cgcgaaagga gcgccggggg caaccctcgt cttcgtcgtc tccgggggag tgccggtgcc
     1441 ggatgctgcc tgaacgggcg accgtcctgc tcctcgccgc cggcctgctc ctcgccgtca
     1501 tctgtcccgg cgtctcggga acaggggggc cgccggagct ctccggcacc gacgccgccg
     1561 ccatcggggc cgccggcaag acaccgggcg acgatacggt caccgccccc ggaaagagcg
     1621 gagacgcccc cggccagaac aaggataagg gcaagagtac accggaagcg acgccgaccg
     1681 ccctgccctc gcccgaagag accgtcaccc cgggagaaga gcagacggag gcgacgccgg
     1741 aggtgacggc aaccgcgaca gccacaaaaa caccccagga taacagccag acggccacag
     1801 acccgttccc ctgggccggg gttgctctgg ccctcatcct gctcggcggt gcctccgtgc
     1861 tcggtgccgg gtacctgaag aagtcacggg gagcagatgc cggcgaagca ccggcgggca
     1921 cgaccatcct ccccggcgca taccggacca cactctccca cccggccggg ttcccgcccg
     1981 aacttgcagg gcggtacggc caggtcgcct tcgtcgggag aggcgggctc gggcaggtct
     2041 tctcggcggt caggcgtgat gacggccgga ccgtagcggt gaagatcccg gtcacctacg
     2101 atgaagcgac cggaaaaacc ttcatgaagg agatgcggtt ctgggaagac ctcacccaca
     2161 aaaacatcgt ggcggtctac tcggtcaaca tcctcccggt gccgttcgtc gagatggaat
     2221 tcctgccccg gaccctggag gaagtggcaa aacccgtccc ggcggagacc gccgcccgca
     2281 tcgctgcagg catcgccgcc gggcttgcct acgcccatga gcggggcgtc gtccaccggg
     2341 atatcaagcc gaggaacatc cttctcacgg acgacctgac gccgaagatc accgactggg
     2401 gcatgagcac gacgctggca gcgacccggg acaccagcat cgccgggttc accctcgcgt
     2461 atgcggcccc ggagcagatc gcccctgagc ggttcggcag gaccgatgca cggaccgaca
     2521 tctaccagct cggcgtcgtc ttctacgaac tggtgacggg gaagaccccc catgccggcg
     2581 agagcctcgc cgggcttgcc ggggcaaccc tccaggagca gccggtcccg ccctcgcgga
     2641 tcgatccagg cctcgcaagc ctcgacccga tcatcctccg gtgcctcgca aaggaccctg
     2701 ccgaccgcta ccagtccgcc ggggaagtgc tcgatgccat cgagaccctg atgagagaag
     2761 caggcgacac gacgccgacc ggacccacgg gggaggagga agcgtgagga accctctcgc
     2821 ccggtatctt gcccgcgcga gggccatgca gttccactac ctgctcgcat cgctcatcct
     2881 tctgctcgcg gtctacccct acgtaggtgc agggccgacc ggcccggccg ccctgaaggt
     2941 gctctcgtcc ttcatcctga tcaccggggt ctacgccgtg agcaaccggc gacggcaggt
     3001 cgtgatcgcc gccctcctcg cggttcccgc cttcggcctc ggctggctct acgtcatcac
     3061 cgggaatccc gtaatcagca ccgccgagag cgtcttcacg ctcctcttct acgccttcac
     3121 cgcgctcgtc ggcctctccc gggtgctcgg ggaacgccgg atcaccaccg acaccatcta
     3181 cggggcggtc tccgtctacc tcctcatggg cctcacctgg gcgaccgcct acggcctcgt
     3241 cgagagcatc agtccgggat cgttcttcag cagccacccg gacggcgcct tcaccttccc
     3301 gacattcatc tactacagtt tcgtcaccct cgcgacgctc ggctacggcg acatcacgcc
     3361 gatcaccgac caggcccggt cgctcgccgt gctcgagacc gtgagcggga ccatctatat
     3421 cgccgtcctg attgcccgcc tggtggcggc ggccgggtgg ctgccggaga ccggccgcga
     3481 gtgacggcaa cctggatatg ggattaaaac aactattttc ggaattcctc cgattaaagc
     3541 tacgaaaatt ctcgttgtta aagcaagata tttattttcg ccaaatcaag tgatgattga
     3601 tacctatgcg aggaaaaaca gcacttctgg ggatcgctct gatggtcctc tgcgccgcgg
     3661 tgatagggaa cgtcaccgca cagttgccgg aggaatccag agacaaagtg atctacgtct
     3721 ccggtaccgg caaggtgacg accacacccg accaggcgat catcgtgttc gcggtcgaga
     3781 ccgagaacgc cgatgttgcg acggcgcagc agcagaacgc ccggcagatg gatgccgtga
     3841 tcaacgcctt aaagaatgcc ggcattccgg caaaagacat ccggaccacc ggctacaaca
     3901 tcatcccggt gaccgagagc gatgacaggc ccctgacaac ctccagggtc aagtactacc
     3961 gggtgatcaa cagcctcgag gtcacgttaa acgacgttag ccgcgccggc gagatcgtcg
     4021 acctggccgt caccagcggc gcaaaccggg tcgaccggtt ctcgttcacc ttgagcgacg
     4081 caaagcagca ggagttccgc tcacaggccc tgaccgccgc ggtagcgcag gcgcggggcg
     4141 acgcaaacgc ggttgctgca gccatcggca agaccatcgt cgacgtcaaa gaggtcaacg
     4201 taggaggtaa ctacgtgccc atggcctacg acagccgcta ctcgggcatg gagaaggcag
     4261 cgggcagcgc tgtcccgacc ccgcttgagg tcggcgagat cgacgtgacc gccaccgtct
     4321 ccatcaccta catcatcggg tgactgctcc ctctttttcg tctgcaggtc cggggcactc
     4381 cgacccccgg gccctacctc tccccaccca atctgcccca tcgggaagca taatcactgc
     4441 cagggagaga ccagaagaca ggagtctgtt ttcatgctca ccatcaccga aatctacaac
     4501 aacatcccct gccgggaggg gctcacaacg gactgggggt tctcctgcct gctcaacgag
     4561 gcgggcctgc tcttcgacac cggggaacgc ggcgacgtcc tgctcgcgaa catgcaggcg
     4621 ctcgggatcg acccggcgtc tgttgggcgc ctcgtcctct cccacgacca ccacgaccat
     4681 atcggcggcc tcgcggcagt tcttgctgcg aacccctcga tggaggtcta tgtccacgac
     4741 gccttctccg caaagacgct tcggctgatc cgggagcata ccgagccccg gatcgtcagt
     4801 gagtggacgg agatcgctga cggcatcgcc gtgaccggcc cgctcgggac cgatatccgg
     4861 gaacagtcac tcgcgataac cgtgccggac ggctatctga tcgtcaccgg gtgcgcacac
     4921 ccgcatatca gccggatcgt cgaccgggtc tcccgggtcg ggccggtgtg gggcgtcatc
     4981 ggcgggctgc acaccgtcan ntcgcagagc ggtacccggc ctccttccgc ccgggcgggg
     5041 ccgggaaggt gcaccggatc tgaacggttc tgaaaaaagg gctcttttga aaccctattc
     5101 tggtcttcct ccctatcctg atgaggccgg tgccagaccc ggcctatccc cgggacgacc
     5161 tttttttctc g
//
LOCUS       DAJB01000160            4091 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_57851, whole genome shotgun sequence.
ACCESSION   DAJB01000160 DAJB01000000
VERSION     DAJB01000160.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_57851"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          663 a         1287 c         1349 g          790 t
ORIGIN      
        1 cccatggtgc gcctcgaggt cgaagcggtc aaaaagccgg agtaacggcg cctcctcgtt
       61 cgatcgcccg gaacgctgtc ccggccctgc cggggagcgg tcacccttcg gcccatcgca
      121 ttcgaagacc ttcggtcttc tcaagctccc gccgctcgca atgctcgcgc cagtcgctca
      181 gcggaaaggt atatatccaa atgcatggta ttcgggcagc atatgcgtat acgggagaag
      241 accctgacgg ttctgcttgt tttatcgctc gtcatgaacg tcttcgtcct ggccgtcgtt
      301 ctcatgccgc aaagcggcct ctccccggcc ctctccaggc ccggaggggc tgatgcgatc
      361 ccgacggcga ccctgagccc tctccctccc gggaagggag cagggaacgg gagtgtggtc
      421 tcgatgcagg tgccggtcat cctccagaag gtcgaggttg accgggggag cccgctcctc
      481 tatgaggagg tgaccgaaga gggggcgatg gtgaatgtct cggttgagat cgtgcccgga
      541 agaggaaggg tgctcgtcca gacaacaccc ctgatgggga tagcctttca ggatgccgcg
      601 aaccttgcgg tcgttgtcgc cgccaaccac tccagcgtgg actttacggg tagcgacatc
      661 atcttcagca tccagggccc ggaggaggtc tcggagatcg acggccccag tgccggggcg
      721 ctgatggcca cgctgctcat ctcggtgctg gaggggttcg agctcaacca gagcgtgacg
      781 gtgacgggat ccatcgatga gagcgggaag atcggcccgg tcgggggaat cctcgagaag
      841 gccgaggccg cagcagcgag cgggaagacg ctcctgatcc tctcggacga gaacgaccag
      901 gtgatcgatt atcaggagga gggccggtcg ttcggcggac tgaagatagc aaggcagcgg
      961 ccggtcgcca ctgatgcgaa agagtacatc gaggagaact tcggcatcca ggtgaaatac
     1021 gcggatactc tcgacgagct gctcgccgat gtccgcgctc ccccgggttc ccaggtcgtt
     1081 gcggcttttt aaaggccgcc acgctctgat tctcctgtcg tgatcgggac ggtgtctacg
     1141 acatcgaccc cgtcgcggaa cacctgcgcg agggttcccc ggtggtcccg gcgatcgaag
     1201 gaaggatcgc ggtcgtcaac aggacctgac tggagagtat ttattctcgt tcgggccacg
     1261 tacccggata gtatgaagtc acagagtctg ctgatcatcg gggttcttgc ggttgccgtg
     1321 attgctgtcc tggcggcaac gctgccggga ctctcctccc cgcagccgcc cggtccggtt
     1381 ccgaccccga ccccgacccc ggcgccggtc cccgggaacg gcacctacat caatgccacc
     1441 tacggctatg ccgtcacctg cccggagggg tggttctaca ccgagtccgg cgagagcgtc
     1501 tggttctcgt cccccgacag gcacgaggag gtccgcgtga acacgaaccc gctctcgggc
     1561 caggaagccg ccgatgaaga gaaagttctc gaagcgctga acgcgaccta tgtgaaggat
     1621 ctcaaggccg ggatcgatgc cgaatgggtg tcgacggaga ggacctccct tgacggcgtc
     1681 ccggcatatg agtcggtctt ctcggtcccc atcgtcgagg aggaccggta caccttcgtc
     1741 atccggtatg ccgtcaggga cgacgtcgtc tactcggtca tgcacacggt attccccgag
     1801 gagtacgaca tccactccgt cgatgtgcct ccggtggcgg aatcgttccg gttcctctaa
     1861 accttttttt tggccgcgtc tctatgcgca gtgcttcagt cgtgctgcct gccgatgggg
     1921 gcggggcgct tcctctccgt gggatgcccc cactcctcga tgaatgggct acacgcccgg
     1981 gctgcgaggg cagtatccgg cgtgcgaggg gtttttaaaa aaggtggcgg gtagtatgat
     2041 gttgttaaga ggtgattcgt tcggcaggac gtctggggag gttccccgtc acctgatctc
     2101 ttccagcggt atatctggag gaagggtcac ttcgggagag atatcgaagt acggttcgat
     2161 gaccgggtcg ttctcacagt cgtggtactg cttccctctg ctcctggccc tcatccagag
     2221 gcggatctgc tggagtagtg tcacatggtt caatcctttc gttcgcagtg gatgggtccc
     2281 actgtgacgc atagccgttc tgatctcctg agaataaatc cattgtgata cgggcttttg
     2341 ctcattggtc cgtaaaatgt ccctgtatta aaaaatagtg cgattatacc cagtattttc
     2401 tgtcccgggc gatcggtccc ttacacccgc cgaaagatcc cgtcgatctt cctcctgtcg
     2461 aacgccacca ctgtcggcct cccgtgcggg cacgtccagg gggtctttgt ccgggcgagc
     2521 tgtgcgagga ggcgcttctg ctggtcgtgc gtcaggtggg cgccggcctt caccgcaccc
     2581 ctgcaggcga cgatacaggt gaccgcctcc cgccggtcgg gggtggtgcg ggacgtatcc
     2641 gtgaggaggt cggcgatcgt ctcccggact gtctcggggc cttcgattgc gccgagggca
     2701 gcgggcaccg cccggacggc gaaggtgtcc cggccgaact cctccaccac gaacccctcg
     2761 ccaagaagga gcggcatcgc gtcccggagc gccgcggaat cctttggggg gagggagagg
     2821 acgaccggca tgatcagttc ctgcgacccg gctccagcat cacgctgctc cacgacctgg
     2881 tcatagagga tgcgctcgtg ggcggcgtgc tggtcgacga ggtagagggt gccgtcggtc
     2941 ccctccgcca cgatgtaggt cgccgccacc tgcccgatcg gctccatggc cgggaggagg
     3001 ttctctctgc cctccgtctc cgtccgccgg agctgcttat cagagagggc gagttcccgg
     3061 tgccccgccg tgtaggcggc ttccggctcg ccggccgtcg gggtccgtgc ctgccggggc
     3121 tctgccgggg agatctgctg ctgcaccggc tcggccggcg cctcgcgtgc gaggtcgtgc
     3181 gtggcgagcg cctcctccac ggccgccgaa accgccggga ggatctcccg ctcccgggag
     3241 aggcggacct cccgtttcgt cgggtggaca ttgacgtcca cgaggtcggt atcgatcgag
     3301 agttcgatga acgccaccgg gtagcggtct ttcgggagga gggtgccgta gccctcccgg
     3361 acggcggcgg cgatcggccg ggaggtgatg cttctgccat tgatgctgat gaagacctgc
     3421 gaggggttcc ccctgctctc agacgggcgg gagacgtagc cgccgatgcg gaggaacggg
     3481 agcctcgcct cgacggggac gagtgtccgg gcaaggtctg cgccgtagag cccggcgatg
     3541 gcgtttaaga gcccacctga gcgctgggta gccatccgct ccttcccgtt gtgcaccacc
     3601 cggaaggcga cctcgccgtg cgcgagggcc aggttctcca cgaccccgta gaggtgggca
     3661 agctcggtgt tcctgctctt caagaacttc cggcgggcgg gggtgttgta gaagaggcgc
     3721 tccacggtga ctgtcgtccc ctccggcgcc cccacctcgc tcttctccat cacctcgccg
     3781 cccctgacca gcacccgggt gccggcgagc gccgcggcgc ccctctggcg ggtgatcagg
     3841 gtcacctccg cgacggcggc gatgcttgcg agcgcctctc cccggaaccc cagggtgcgg
     3901 atggcggaga ggtcggcgat atcnnggtgt catcccctcg ccgttgtcgg tcacccggac
     3961 cttcgtgatc ccggcgaggt ctgaagagac gtcgatgagg atgcttgttg cgcccgcatc
     4021 gatcgcgttc tccaggagct ccttcaccac cgatgcaggc cgctccacga cctcgccggc
     4081 ggcgatctgg t
//
LOCUS       DAJB01000161            4173 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_92134, whole genome shotgun sequence.
ACCESSION   DAJB01000161 DAJB01000000
VERSION     DAJB01000161.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_92134"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          727 a         1462 c         1311 g          673 t
ORIGIN      
        1 cacctcttcc atgtccagac ggtcgggggc acggtcggga tcgagtatca cgggaagtcg
       61 ctcgtcgcct accgggtcga gcggacgcac ctcatggccg ccgatgtcga tgaagccacg
      121 gcgctcgcgg cggcccagat ccaggacggg gtcgcccggt tcgtggagga gcagggatgg
      181 ttcggggtcc ggctcacgct cggcaccccg cagatcatcg ataagccgca cttcgccttc
      241 gcctcgaaga ccgcccgcac ggtgctcgac gccgggcacc agctcacgac gcccgggatc
      301 tactgcgacg actcgctcgc ccaccacggc cggccggacg ccggggagat cgagaccacg
      361 gacccgatcc tcgcggacca gatcgaccgg gggctgcgga acgccctgaa catcgagccg
      421 atcgtggagc ggtcggtcgc ccggggcatg gtcgagcaga gcaagacgat cttggacggg
      481 ttctgccagc agctcgaccc gatccagcag acgctcgggt cgatctacga gaccgcgaac
      541 accgtcctcg cccacgtcca ggggggaacg acgctggagt atcagttcac ccagatcgtg
      601 acgctgctca cccataccct gaacgagatc aaggacctcc ggggccgggt cgcggacctc
      661 gaagagcagc agacccgtaa aaatgtgccg aaagggaaga aaaatgtcgc cagaacaatg
      721 ccacaggaag gccgggacgc ggggggcgcc ccggcccggg gtcccgatca cggaccagaa
      781 accggatgcc gagcacaagg tctgcctgaa gtgcgggctg gtctaccaga aacaccgggt
      841 cctgaagtac cgctacatcc cgctgggcct gcaccgggag gtgacccggt atgtctgccg
      901 ggagtgcttc gcccggatgc cggcggcgga acgccggcag gtcgtcgtgc ccgccggaag
      961 aagaaaaagt agccgggccg cggtcgtcaa ccccgctgcc gtcgcgggtc tggcccggct
     1021 ccggaagacc aaaggagaga gcccgcaggc catcctggcc gcgcaccacg agatcctgaa
     1081 agacgacccg cagcggctga gcagcgcgtt catcctctcc ctttcccgga tgccgcgagg
     1141 aggcgagtcc cgtgagtaac ccgcggaagt actgggaccg ccggtatgcc gccgggggga
     1201 cgtccggcct gggctcggtc ggaacgctcc gggactggaa gcatcaggtt atcctgtcgt
     1261 atgccgggac ccccgcctcg gtgatcgatg tcgggtgcgg agacctctcg ttctggcacg
     1321 ggtgtccccc ggagtcctat accgggatcg acctctcgac cgtcatcatc gcccgcaacc
     1381 agcgccggta tccagatcgc cggtggatct gttcctccgg gagcgtccgg cccccggtca
     1441 ccgccccggt cgtgctctgc cttgacctgc tcttccacct cctggacgag accgagtacc
     1501 gccggacgct ggaaacgctc tgcgcctgct cggaacgctg gatcttcatc tacacctggc
     1561 acacgaaccc gatcccggcc atccgctggc agatccgggt agaccacctc cggcacctcc
     1621 ggttgcgggc gtttgccgca tcgttctggg acctcacctc cgaccatgtc tacgaacagt
     1681 tccgaaacct cagaaccgac ctcccgctct tcaccgcggc agggttcgac ctcgaccggt
     1741 tcgtgcccta cgaccaggtc ggcgggatgt atgtcttccg caagcgggag gaaaagcatg
     1801 aagatccttg aagtcggcgg gtgctgcgcc ggggttcccg cccggctggc cgacctcctc
     1861 gacacccggc acatcacgtt cgggccgacg gggttcgctc ctgcaaagca gactgtgcag
     1921 tttggggagg cccggcggtt cgaggttctc aaccgagtcc agcacctccc cgcattcctc
     1981 gcggcggctg agtcgtatga tgtcctgcac ttccactacc gcacctacct gccgcagttc
     2041 ctcgacctcc ccttttggcg cttgcgggga aaacgggtct gggtctcgtt ccacggcagc
     2101 gacatccggg gccgggccct caaggccgca aaccccctgc tccggctcgc agaccggatc
     2161 ttcgtcagca ccccagacct tctcgatacg ctgccggacg ccgaatggct gccgaacccc
     2221 gtcccggccc ttgaccccgc cccgctgccc gacaacgaga tcccggtcgt ggtccactgc
     2281 ccatcaaacc cggccctgaa agggaccgcc gtgatcgagc aggcggtcgc gtggctcaaa
     2341 cagcaggaag gacgggagtt cgtctaccgc cgggtcacgg ggtgctcgca cgaagagacc
     2401 ctcgcggcca tgcggggtgc cgatatcgtg atcgaccagg ccctctatcc caggctgtac
     2461 tgcatggtct ccatcgaggc gatggcgctg gggcggccgg tcctcgcggc agtagaccgc
     2521 cgcacgctgg cccgccttcc ccccgactgc ccagtcgtct cctgcgggac cactcaggaa
     2581 accatcgcgg cggcgctcgc ctatctgctg gacgcccccg gccggtgtga cctcctcggt
     2641 caccaggggg tccagtatgt tgcccgggtc cacagccccg acgccgtgag ggaggtgctc
     2701 ctcggatgaa atattcagac ttcatggttt gggcagacga tcttatccag atctacctcc
     2761 gctgcggtcc ccgcccattt actcgtgccg aaatcagtga cataactcac cgaggaacca
     2821 tgaatcggta cgccgaacga ggctattttg ttcgtgttcg gaactccgag ggttcgcccc
     2881 tcaaaaaagg cgtccggggc cgtgcaaacg tctggcggtt ccatgatcgc attgcttgga
     2941 agtgtcaatc ggtaattgca gagcatgagc gattggaggc agatcttgga tggaatcgcg
     3001 atctaccaga cgttttgtgg aggtcccctg aatggcgcag cgggggggaa gatctggagg
     3061 agagatcatg atcgtctgcc acctcacgac tactcacccc ccaaacgacg ccaggatcac
     3121 gcgggagagc gactccctgc gccgttccgg gtgccgggtc tccgtcgcca ccatcccgcc
     3181 ggggtacccg aaatggcaac acccgctcaa actcctccgg ctcgtgggag agtcgttccg
     3241 ccacaatgcc gatatctacc actgccatga gcccgatgcc cttgttgtgg gactcatcca
     3301 tcgtctgcgg gggaagaagg tcgtctatga cgtccacgaa cactggccct ccgagctccc
     3361 gcacgacatc aacgccccgc aaaccatcgc ccccctcatc gacccggtcg aacggtggct
     3421 ggcccggcgg gccgatgccg tgatcgccgt ctcggagagt gtcggggccc ggttcgacca
     3481 ccccgtgatc ctgccgaact acccggaccc cgccacaact ctcctccccc cgccggcgac
     3541 cctcaatctg cacgccttct cctgcatcgc cgccaagctg cacaccttcc acggggtccc
     3601 ggaagccctc gcggcggtgg atcgtctccg tgccatgggg tgggaggatg cccggctcac
     3661 gctgatcggg aaccgggccg tcgccctccc ctccgccgcc ccggtcacct gcaccggcta
     3721 cctcccgcag gcccgggtcc cggccgctct ccaggccgct ggagtggggc tggtcctcct
     3781 ccagcccgag tacgagaaca tccggatcgg cctcccgaac aaactctttg cgtacatggc
     3841 cgccggggtc ccagtgatcg cctccgccct gccggagatc cggcgggtgg tcgaggacgc
     3901 ccggtgcggc ctcctggtgc cgccggggga cgtggacggg atcgtggacg cggcgatccg
     3961 gctcgcggat caccccgacg ccgcccgggt gatgggggag aacgggcagg tcgcgatccg
     4021 cgaccgctac cactgggcgg cggtcgaagg gcggctgact gaagtgtacc gccagatcga
     4081 aacgaggaac aacctatgat cgaaaactac actgttatcg agcggaacga agccgagacc
     4141 gtcgccttgg tcgagggcga gccgggggac gcc
//
LOCUS       DAJB01000162            8804 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_12185, whole genome shotgun sequence.
ACCESSION   DAJB01000162 DAJB01000000
VERSION     DAJB01000162.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_12185"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1583 a         2866 c         2951 g         1404 t
ORIGIN      
        1 cccttcgagg gcgacccgcg ctacgtgctc cgcatggcga tggaggacgc cgcgaaggac
       61 gggtacgtct tcaacaccgg gccggagctg gagttcttcc tctttaagat gcttgacggc
      121 cgaccgacca cgcagttcca ggacgccggc ggttacttcg acctcgcccc caccgacctt
      181 gcggaggacg tccggcgcga gatcatcctc gccctcaccg agatggggtt cgagatcgag
      241 gcgtctcacc acgaggtcgc cgagagccag cacgagatcg acttcaagta cagcgacgcc
      301 ctgacgactg ccgaccgtgt cgtgacgttc aagttcgcgg tcaagacgat ggcgctgatg
      361 cgcggcctgc acgcatcgtt catggcaaag ccgatctacg gcatcaacgg gagcgggatg
      421 cacgtgaact gttcgctcgc gaaggatggt aagaacgcct tctacgaccc cgacgccccg
      481 ctgcagctct cagagacctg catgcatttc atcggcgggc tgctccgcca cgcgccggcc
      541 atcacccggg tcgcaaaccc gacgatcaac tcctacaagc ggctcgtccc cggctacgag
      601 gcgccctgct acgtcagctg gagcacgagc aaccgttcgg ccctcgtccg ggttccggcg
      661 ccccgcggca acagcacccg ggtcgagttc cgcagccccg acccgacctg caacccctac
      721 ctcaccttcg ccgcgatgct caccgccggg atggagggcg tccggaagga gatcgagccc
      781 ccggccggtg ccaacgggaa catcttccac atgacgaccg ctgagcggga gcgcgacggg
      841 atcgggacgc tgcccgggag cctcgccgag gcccaccggg acctgctcgc cgacgacctc
      901 atctgcaggg cgttcggccc ccacgtcgtc gatgccctca cgagtgtcgc agaagccgag
      961 tgggatgctt tccggacggc ggtccacccc tgggaactcg accgctatct ggcggcatat
     1021 taaccttttt ttgctgcttg aagctccgga cgttccgtcc gtcgctctcc gcaacgttct
     1081 taatagagca ggcccagatg catcgcaccg gccgaccggg gagatctatg acgcagatga
     1141 aagatatcga cacgatgcgg agggcccggg atatcgacgg gctgattgga gcgcttttag
     1201 acccggccga ggctgtgcgg ctgaccgcgg ccgaggccct cggcaccgtc ggcgacgaac
     1261 gggcgctcga tccgctggag cggctgaagt tctcggactc taacgtgggc gtcaggcggg
     1321 ccgcgtcgct cgcgcacgcc cgggcggtag ggaggctcgc ggagaagaag gcagtggaag
     1381 gctggctcct tgatacgtag gcagacggga tcctctcctc cacgaatacg gacgggccgg
     1441 ggtcacctgc agtccgcccg caaaggcata tataccgccg gggcgccaat gcgggagatg
     1501 atcaggagat tgctatggcg acactggacg atatcgatac cctgcgcgac ggtcgggaca
     1561 tcgacgggct gatccgggct ctcgacgaca ccgacgagca tgtccgggcg caggcggcgc
     1621 tttccctcgg gacgatcgcc gacccgaagg cgagagagcc gctcgaccgt atgcgaaacg
     1681 aggacccgag cccctcggcc cgggaggcgg ccgggaccgc gtataaatgg ttggtcggcc
     1741 ggctccagga ggtcgaggtg tcccggagac ccgggacccc gccgaaccgg tagaaccggc
     1801 tcacagcacg acgaagttga gccagaggag gcccgacccg atcgctacgg tgacgaggag
     1861 cacgagcatc cccaccttca ggaactccac gaacgatatg gagacgccgt ttcgtgccgc
     1921 gatcccgagc acgacgacgt ttgccgacgc cccgatagcc gtgccgttcc cgccgaggca
     1981 tgcaccgagc gagagcgccc accagagcgg gtaggtgtcc atcgaggtgc ccatattctg
     2041 gatcaggggg atcagggtgg cggtgagggg gatgttgtcc acgattgccg aggcgatcgc
     2101 cgcgaaccag gcgatgatca gcatggcctc gccctccgag tggacgttct cgaccacaaa
     2161 cccggcgacg gccgcgatcg ttccggtctc gacgagggcg ccgacgacga cgaagagccc
     2221 gccgaagaag aagagggccg gccactcgat cttctcgaat atctcctccg gcggctgcct
     2281 gctccagaag agaaggatcg atgcgccgat cagggcgatg agagcgggtt ccaggccgag
     2341 caggtcctgg gcaaagaaca tcccgacgac gagcacgatg acggcgatcg acttcttgaa
     2401 gagcgaccgg tcccggatcg cctcctgctc gttcaagttc tcaagcgtct ttgcgatccc
     2461 ctcctgctcg gttgccgaga caaggagctg ctttctgtag ataaggaaga gcatccccat
     2521 gacgacgacc aggttgacga ccatgatcgg tcccatgttg acgatgaact cgttgaacgt
     2581 caggccggcg gcggagccga tcatgatgtt cggggggtcg ccgatgagcg ttgccgcccc
     2641 gccgatgttg gaggcgaata tttccgcaat caggaagggc aggggggtta tccgcatcac
     2701 gttcgctatg tagagcagca tcggcgtgag gaggaggacc gtggtgacgt tgtcgaggaa
     2761 ggcgctcgtg acggcggtca cgacagagaa gagcagcaac actcgtatcg ggctcccttt
     2821 cgcaagtttc gccgtcttta tggcgatata ctcgaagagg ccgctgccgc gggcggtgtt
     2881 gacgatgatc atcatcccca tcaggaggaa gatggtgccg aggtcgatgt actccgggat
     2941 cttctcccag gggacgatat ggaggaagac gaggaccgag gcgccgagca tcgccgccac
     3001 tgcacggtgg atccgctcgt cgatgatgag cgcgtaggtg aagagaaaga ccacgatggc
     3061 gatcagttcc gggccgatca cgccttcgca ccccggcgac ggttggtgct acgggtggcg
     3121 gggcgtgcaa tgaacgtctg aggaattgtc ataggatccc aaaacgaata gtcaggaatc
     3181 agattcgcat ggctgcccca taatcatgcc gctccctaca gtccggccgg aggatgaaac
     3241 ccggcacctc aggagggggg actccatctc agagggcggc ggacggtcgc cccgggttca
     3301 gtccggcaca cggacatctc cggggccgga gccctctacc cccctccccg cacgtagcag
     3361 gaggatctta tagacctccc cggcgaggag caccggcagg ccgagcaggg cgagcagccc
     3421 ccagtcgccg ggggtcagcg gtgcggtccc gaggacggtc tgcaggatcg ggacgtagac
     3481 caccagcgcc tggagggcaa ggctcccgag gatcgcgagg agcagggtgg ggttcgagaa
     3541 gaacccgatc cggtggaggg ggaaccggaa cgaccggaag ttcagcacgt tgtagagttc
     3601 gaagatgatg agcccggtaa acgccatcgt ccgcgcccgg tcgaggtcga cctcgtagag
     3661 gctggagaag acgtagatcg tcaggagacc gagccatgcc ccgaggatga ggacgacgag
     3721 gtaggcatct ttggtgagga tggactcccc ggggtcccgg ggccgcctct gcatgacgtc
     3781 ttgctcggcg ggctcgagac cgagcgcgat ggcggtgacg ccgtccgtga tcaggttgat
     3841 ccagaggatc tggatgggga tgaggatcag cggcaggccg aggaggagcc cgccgacgat
     3901 cgcaacgagc tccccgatgt tggaggagag gaggtagcgg gtgaaccggg cgatgttgtc
     3961 gtactcccgc ctcccctcct cgacgccggc gacgatgctt gcgaagttgt cgtccacgag
     4021 caccatgtcg ctcgactccc tggcgacgtc cgttcccttg atgcccatgg ctatcccgat
     4081 gctcgccttc ttcaacgccg gagcgtcgtt gacgccgtcg ccggtcatgg cgatgaccgc
     4141 acgctcccgg gcaaggatat cgatcacccg gagtttgtgc tccgccgtga cccgggagag
     4201 gacctttgtg gtccggaggc gactcgcgag ttcgtcgtcg cccaaagcct cgaggtcggc
     4261 cccggtcagc gccccctcgc tcgagaggcc gaggtcccgg gcgatggcgt aggcggtcag
     4321 cgggttgtcc ccggtgatca tgatcacgtc gatcccggcg ctccgacaga ggcgtatcgc
     4381 ctccgccgct tcggggcgtg gagggtcgcg gatcccggca aacccaagaa agatcaggtc
     4441 ttcctccacc gtctcctcgg tcaacaaaag accgtccggg agcgggcggt atgccgcccc
     4501 gagcacccgg agcccgccgg acgcatattc ggcgatctcc gcgaggattg catcgcggag
     4561 gtcgtcggtg agcaccacga cagaatctcc ccggagcagc cgggacgacc gggcgagaag
     4621 cacctccggg gctcccttca cgtaggcgac gtctccctcc tcttcccgat agatgatcgt
     4681 catccgcttc cgggtggagt tgaacgagaa ctcccggacc gcctccggag catcctcgcg
     4741 ggagaggccg gctttggccg ccgcgaccac gagcgccccc tcggtcggcg tgccgaggat
     4801 ccgccacccc ccttccgtga gagagacggt tgcatggttg cagaggagca cggtgcggag
     4861 gaaccgggcg agccccggtt tcgccagggg atcgatgggg ttgcctgcaa cgaggaactc
     4921 cccctccggg gcatacccgg ccccggtcac cgtcacctcg cactccggcg tcttcacccg
     4981 gacgaccgcc atctcgttcc gggtgagggt ccccgtcttg tcggtgcaga tcaccgagac
     5041 cgcgccgagg gtctccgatg ccgggagatg gcggatcagg cagttcctcc gcatcatgct
     5101 cttgatgccg agggcgagcg tgagcgtcac gaccgccggg agaccctccg ggatcaccgc
     5161 caccgcgagc gagacgccga tgaggaacat ctcgaggagt tcccgctgct gcagaagacc
     5221 gacggcgacc gcaaccaccg cgataccgat ggcgatcagc ccgaggtccc ggccgaggcg
     5281 gtccatctgc cgggagagag gcgtcgtctc gtcttcgacc cgctgggaga ggccggcgat
     5341 catgccgaac tccgtctgca tgccggtggc gacgaccacg ccgcgtcccc gcccgttgac
     5401 gaccgtcgtg cccgcaaaag ccatgttgct ccgctcagca aggctggtcg gggaagggag
     5461 cgaacccggc gccttatcga caggcgacga ctcacccgtc agcgacgcct catccacctc
     5521 gagcgacgtc gcctcgacga tgaagaggtc ggcgggcacc cgctctccca tctcgaggac
     5581 gacgaggtcc cccggcacga tctccgaggc atcgatctcc tgctgccccc caccgcggat
     5641 gaccacggcc cgctgaacga gcatcttctt caacgcctcg atcgcctcgc cggcctgcca
     5701 ctcctgggag aacccgagga tggcgtttaa gacgacgatg atgaggatgg cgacggcgtc
     5761 caggtacgcg ccgacgaaga acgagacgcc tgccgcgacg atcaggatga cgatcaggac
     5821 gctcttgaac tgccggagaa agacctggag gcgggtctcc cgcgcctcct cgcggaggac
     5881 gttcttcccg tagcgctcca gccgccccgc cgcttcttcc gcggggaggc cggatgggcc
     5941 ggtgccgagt tcccgctcga cctcatcgag cgagaggctg taccagtcgg gctgaggtct
     6001 tccttctgcg ggggggacct cccggcggtc ccgattcggc acaatcatac atctccggtt
     6061 ctgcgccgcg ctataaaaag atatagaagt cgctcgaacc tccagagaac ggccgcagtg
     6121 gcggctccgg cggtcgggtg gataggcccg ggcctgccat ccttgcggta acattatatc
     6181 tcccggccga acagatcggg ggccgggtcg ggagtccggc ccggggagga atgacgatga
     6241 gcagaatagc ggtcctgatc acggatctgt ttgaggatgt cgaatacacg cgacccgcag
     6301 aggcgtttct ggaggccggc cacgacctcg tccacatcgg cctctccgcg ggagagaccg
     6361 tgcgcgggaa gcaggggcag gcagaggtca ggatcgaccg ggccgcatcg gaggtatcgg
     6421 cggacgactt cgacgcgctc ttcataccgg gcggctactc gcccgacaaa ctccgtgccc
     6481 acgacgcgcc cgtcgagttc gtgcgtcgct tcgtcgagag cggcagaccg gtcctcgcca
     6541 tatgccacgc gcctcaactc ctgatcaccg cgcaggtgct cgagggccga aagatcaccg
     6601 gctggaagtc cgtcgcccag gatatcagga acgccggggc ggagtacatc gaccgggagg
     6661 tggtggtcga cggcaacctg gtctcgagca gaaagcccga cgacatcccc gcgttcatcg
     6721 aggcgtcgct tgcgaaactt gaggagaccg gcaggaagac gaagactgcg gctgcagcgc
     6781 agcggtgagg ccaaagcccc gccgcacaaa ctctttatgg atgcgaaccg cacccactct
     6841 ttgagatgaa tcctgcaggt ctgccagact caggggaatg gggcaggctt gcgatcatcg
     6901 ccttcgtcac ggcgatctcc gtctttctcg gcctcttctt cggcgccgga ggtgcgtata
     6961 cctgcttatt ctacccggtg ctcgtcctcg ccgcattctg gtaccggaga cgggcggtat
     7021 atctcgggct cctcctcgca gcgctccaca tcctcatcga gtatctctcc ctccgccctc
     7081 tggctccgga tgccctcgcg cgtgcggcgg tcttcgtcgt cggcgtatac ctcctcggat
     7141 acctcttcga ccttgcgggc aggcgtgccg aaggcctgca cttccgcgtc ggcgagcccg
     7201 gcgcacccgc gtgcgaccgg gatacgcgaa ggttgatctc ccggttatcg agccgggacc
     7261 cggatacccg ctaccaggcg gccgggtgcc tcggcgacgc aggcgtttcc gctgcggtcg
     7321 ggccgctcgc cgctctcctc gccgacccgg agaccggtgt ccgctggaag gcgacggagg
     7381 cgctcgggag actgggatct ccggccatcg ggcccctcat ggagagcctc gcgagcgaga
     7441 acgtcgatgt tcggtggatg gccgcggttg ctctcggcga tatcggcgag gcggaggcga
     7501 tcccggcact gatggaggcg ctcaacgatg aggataccta cgtccggagc agggtggccc
     7561 tcgcgctcgg ggctatcggc gaggccgcac gaggggatct catcgccgcc ctccacgacg
     7621 ggaacgagcg ggtccggtgg ggtgcagcgc tggcactcgg gagtatcggc ggagaggagg
     7681 ccgcgacggc tctcattaat gcgctccacg accccgacgg ggatgtgcgg cagcgtgccg
     7741 tgggtgcgct cgccgatatc ggcgagcctg ccgttccccc ccttcttgcg gcgctccgga
     7801 cggacgagga agagttccgg caggaggcga tcgccgccct cggcctggtt ggaaaacctg
     7861 ccgtcgcctc gctcgttgcg gcgctgcgtg gccacgacga ctggcgcgtc cgagcaggag
     7921 ccgcgcgagc tctcggggag atcggcgagc ggcggtcggc cgacatcctg atcgtggcgc
     7981 tcgatgacga gcgggaggag gtgcgcgagg ctgcacggga ggcgctcggc agcatcagga
     8041 agacataaca ccagcgagag agcacctgaa cgctatgact gacgacaccc ggatcgagga
     8101 actgatcgcc gaactccggc agggccctct cgcggtgcgc cgggcggcga ccgccgagct
     8161 cggggcgagg ggagaggcgg cagtcctgcc gctcatcgcc gtaatgcagg tcgagggcaa
     8221 cgacgtccgg tggtatgccg cccgtgcgct ggtggagatc ggcaggccgg cgatcggccc
     8281 tctcctcgcg gcgatgcgcg ccacggacga ccgggacttc cggcgatacg ccacggcgac
     8341 cctgtcgggg ataggcgaac ccgctatcga gcccctcatc ggcgtcgtcg agaacgccga
     8401 cccggacctg cagccgtttg ccgccatggc tctctgcaag atcggcgagc cggcagtcga
     8461 accgctcctc cgcctcatgc gcaatcagga cccgaagacg caggagcgtg ccgcgctcct
     8521 cctctgcaag atgggggagc cgggcgtcgg accgctcacc gaggcactga agaccgggag
     8581 cagatccgcg aataataaat gatcggagaa ccccggatca gtaaggtctg atggcacctt
     8641 acacgatgaa gggccccctc ctgccgctga tggagaggga ctttcaggaa cccgggaagt
     8701 ttctggttgc aggtactgcg gtccttgccg ccctcattgt agagcccccc ggcaccaacc
     8761 tcatcctctg gttgcttctt tttatctccg cggggctcgc gggg
//
LOCUS       DAJB01000163            2927 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_10682, whole genome shotgun sequence.
ACCESSION   DAJB01000163 DAJB01000000
VERSION     DAJB01000163.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_10682"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          534 a          937 c          901 g          555 t
ORIGIN      
        1 tccctaatcg cagaatagtt tgggtacgat gatgcggcag atctcctgca gaagcgcagg
       61 ctcttgcgtg gcccgcacga cagcctggtt gcactcgcgg agaatgttga gcgtgcggtt
      121 gacccgggcc agctcggcgg tgcgctcctc cacccgccgt tccagcccgg cgcgggtttt
      181 ttgcagcgcc tcttccgcct gcttgcgcct ggtgatgtcg agcacaatcc cctggtagtg
      241 agtgatggtg ccgttttgat cgcggcggat ccgggttcga tcgtcaagcc agcgaacttc
      301 gccggatgct gtgagaaccc ggtactcttg aacgaactcg tcgcgccctt cgcggctgta
      361 ccgggagacc tccgccgtta cctgttccag gtccccgggg tggatgatcc gggcgtaggg
      421 aatgcggccc gatgtgaaat cggccggtgt atagccaaac tggccgacgt tatcggatac
      481 gaattcgacc ggccatccct ctgccgcacg ccagagaaac gcaacggccg gacttcgatt
      541 gacgatcttt tccagctctt ccagtaccct ctccggctcc tcttccacca gagtgcgccc
      601 ggttacattc attgccacac cggtgatgac cgggaccacc gggtcctcgt gcgctcgttt
      661 cctgccatcc ggtatcctct tgtattccct gtggtggatt tcgccctctc cccggtcttc
      721 aggagaaggg caggtgccca tgagtagccc ttcccccggt actcaagtgt ttcgtccatc
      781 catcggtcgg cctccccgcc ccccttaacc gcaggttgcc gtttgcggac ctggcgaacg
      841 ctcatcttca tgctgccgat ctcaaccccg ggacacttcg tttcggataa tcgaaaaata
      901 tttccttcat acaataacga taaccccgca cgcttacccg ggaccggaga gggcctacct
      961 cccccccgca ccggaggcca cccgctccgt caaccattcg tagaatttgc ggtgccagaa
     1021 cggcacgtct atcggccagc agagcgactg ccatgccgcc cacatgagcc tctcagacat
     1081 gctctgccac tcggcggccc gcgaggccat gtcgtcggcc ggctgcccga tcgcgtgcag
     1141 gatctcgtgc cagatgcgga ggacgatcgc ctcgtcatcc ctgtcctgcg acgtgacccg
     1201 gacgctcgct cgtttatcca tcccgccccc gagcgcccgg gcggggatcg cgtcgtcgaa
     1261 gacgtagacg gtcttttccg ggaccgggaa agggaagccc ttccacatcg ggtccttctg
     1321 gtcgaagtag cggacctccg gcgtgcctgt ccggaccgtg aactcgaaga acggcgggat
     1381 aagccgcagg tgcggctcaa gcctctgccg cagcgcttcg gtcttgaaga agaggtcgaa
     1441 gtgaggcatc agggccgcat gggtgatagg gggtgataaa tattcagttc atggggggcc
     1501 caccggaaca gggggttact gccgaccgga ggggctccgt tctgccccgg gctgaacaaa
     1561 caccaccagg tctccgggtg tgcgcggttg cgccgcatcg gtcccggccg aaggttccgg
     1621 catggctggg cccgaatcac tgcaccctgc cgcactcgct accccggctc ctgcatcccc
     1681 gtttcagggt gtcgtttcgc gggcaccctc ccgcaagagt ttttccgcga gtgctccctg
     1741 ctgctcgtcg atccaggtcc gggtccagtc ccgcaggtca ttgatgccgg cggacccctg
     1801 cttgctgccc atcgcccgca ggtagccctc aagttcgtcg gccagcccaa agatcagcgt
     1861 ctcagacgct tcttgcgata acggcccttc cgcccggagg cgggcagcgc ccaggtcgag
     1921 aaaatgttgc aactcgcctg ttgcctcgag gaccgtggtg agatccagtc tgacagtagg
     1981 ccccatgaag aggggattga cgcacccggt aataacttca ttcattttgt tccgggcaag
     2041 gaggatgcac ctcttcccat ataatccccc attccaggtt aattcagccg gagtgcgctt
     2101 ctgcgccgca aacgggcagg gtgggtcggg cgggtcccgg gcctgcaggg acaaaaccat
     2161 tttaaggact ccgggcactc agtgcacgcc ccgcctcagg tgacgtgcct gagaaagggg
     2221 cgatggagat gaagaagatg caggagagat cggaggtaat tctggagcgc aggacgcgga
     2281 cggccggcga agagtggttc atgtcggccg acgacatcct ggacaagaac gtgatcaacc
     2341 cgcagggaga cgacctcggg gagatctcgg atatgcgggt ctccttcccg gagggcaagg
     2401 tgcagtacct ggtgctcaag tacggcggca tgctcggcgt tggggcaaag aggtttgcga
     2461 tcccgcccga ggccgtgatc taccgcccgg gggacgacaa tttcgtcgtg aacatcgaca
     2521 agcagcggct ggacgacgag cggggcttcg atgaggacaa ctggccccgg gaagcggact
     2581 ttgacctgat ccagtcggcg ggggcgatga cacccccgag ccgggaggag gtggaggcca
     2641 tgaagcggca ggagactccc ccgcccgagg tcatcacgac tgagcgggtc gagacacggc
     2701 cccggcagag ataaccctgg acgaagagtc cagggaacgg atactttttt tggcgggcga
     2761 cccgcggcag gattacccgc gatgaacgag ccacaccggc acctgcaggc ggtccgccct
     2821 gcaccaggtt tttataccgt gagaccactg acgccgactg tccgccccgg catgccgggg
     2881 taggaggcct ataccatgca gttcgtcaac tacccccggt taaaacc
//
LOCUS       DAJB01000164           17597 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_20287, whole genome shotgun sequence.
ACCESSION   DAJB01000164 DAJB01000000
VERSION     DAJB01000164.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17597)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17597)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17597
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_20287"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    7863..7897
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3390 a         5554 c         5383 g         3231 t
ORIGIN      
        1 cgggcttacg agatgctccg cgactgcgtc gtctgcccgc aggagtgccg cgtgaaccgt
       61 gtcgaggacg aaacggggtt ctgccggacc ggcctcctgc cgggggtctc cagttacagc
      121 ccgcacttcg gcgaagagcc cccgctggtg gggaggaacg gatcggggac gatattcttt
      181 tccggctgta atatgcggtg cgagttctgc cagaacttcg aggtcagcca gtgcggggtc
      241 ggcggcgcgg tcccgtgcga agacctcgcc gcgatcatgc ttcacctgca ggaccgccga
      301 tgccacaaca tcaacctcgt ctccccctcc cacgtcgtcc cccagatcct ccgtgcggtc
      361 gccctcgccg ccctccaagg cctcagcctc ccgctggtct acaacagcgg aggctacgac
      421 tccgtggata ccctgcggct cctcgacggc gtcatcgaca tctacatgcc ggacgcgaag
      481 tacgggcggg acgacgtggc gctggaactc tcccatgccc ccgactacac cgcccgcatg
      541 cgggcggctc tcgcggagat gcaccggcag gtcggggacc tggaggtcag agacggcatt
      601 gcggtgcggg gcatgatcat caggcacctt gtgctccccg gaaacctcgc gaacagcgaa
      661 atagtgatga agttcatcgc cgacgagata tcgcgggact cctacgtgaa catcatggcc
      721 cagtaccacc cggcatggag ggcggccgag ggagggagga gcccggtgct tgcggcgctg
      781 cagcgcccga tcacggcgcg ggaataccgg tatgccatcg attgcgcaaa ggagaacgga
      841 ctctcacggg ggttcccgtg agagacgggt ggtaccgcca ccaaaagaat attcgttcgg
      901 gcagcccagg atcgtgtggt gcagggcagg agagagtccg ggacccgtta cgcggcaatg
      961 agcgatatcg ggagcaggga acagaatgag gacgcttact ttgccgaccg gatggacggg
     1021 tatcatgtct tcgccgtcgc ggacgggctg ggggggcatg cccgcgggga ggttgcgagc
     1081 aggatggcgg ttgaaatcct cgcggaggct gcccgcgagg gtctgccgga gactccgccc
     1141 gccgcagtcc tcgatcgcgc gttccagcgc ataaacgccg cagtgtactc ttataaccag
     1201 gagaagaacc tgaacgccgg aacaacgctc tcggcggcga tcgtcagcgc atcaggcagg
     1261 tgctggatcg ggacggtggg ggacagcagg acgcacatca tcacgtcgac gtccgtctgg
     1321 catacgcgcg accagagtta cgtccagggt ctcgtggacg cagggatgct ctcccctgcg
     1381 gaggcgatgg tccacccgaa aaagaacatc ctgacccagg cgctcggcct cgcggcccgg
     1441 gtgcagatcg accttgatga gcgggaactc gcaggggcgg ttctcgtcat cagttcggac
     1501 gggctccacg actacgtccc ggcaaggacc atccagaaga tcgtgctcgc cggcgatccc
     1561 gatacggcat gccggagact gatcggtgcc gccagggacg cggcaagcac cgataacacg
     1621 acggtgatcg tcgcgagata ctaaacgccg tccgcccgcg ctcctcatcg cacctctggt
     1681 gcctaaactt cgactccgtt cctgcggagc gccgcaacga ttgcggcacg gacgccgtcg
     1741 gccgcctcgt ctccgagagc tgcacgcaac agcccgatcg cctccccgcc gccgatcttt
     1801 ccaagggatg aggcagcact ccgtcgcacg aactcgttct cgtcgcgctg catgaccccg
     1861 aggcgtgtaa ccgcgcgcgg gtcgccgagc agtccgagcc ccttcgccgc catgtaacgg
     1921 acatggtcct tcccgtcgtc gagggcagtg gtgagaggcg catacgcttc accgtcgccg
     1981 atcagcccaa ggacctccgc cgcacggtag cgcaccttcc agtcgctgtt ctgcagcaag
     2041 gctatgcagg cgggtatggc cgatcgtccg gccgccgcga gcccgcacac tgcctccgcc
     2101 cgcaccgtct tatccggatc ggataatcgg gacaacagat tctgtagatc ctgctcattc
     2161 aaacgatcgt cttcagtctt gctctgcgtc atcttgcgtc acatccgtca tgtaagcccg
     2221 ggcttacatc acacgttgag gcggatttgt aataagttct taggaccgcc gggacccgtt
     2281 gcaggcacgg tatcccggcg ggcgccttct gccgtcaggt cagaaacccg gaggcatata
     2341 tgaccatact tgcgaaaaca agaagaccta tcccgaggat gagcgtcccc agactgtcga
     2401 ataggggacc gctctcaatc cattctcctg gaacgtgcat ggcaccggat actgcatcaa
     2461 ggtatatata ttttatccaa agaataaaaa taaaatctac tatttaccct ttttcatcgc
     2521 gccgactgcc ggacaggagc ctttgaggat tatcgccgga ctcacgagac gttccggcag
     2581 atccagggga gtgcggcccc cgccgtcatc accggcgaag aaagggtagt gccctacgaa
     2641 acaaccgata ataacgttgt tgagatccca tccgggtgtt cccgattgat tgcaatccgg
     2701 ggcacggctt tccattgggt atcgccatga ctgcccccgc cccctggggc gggggttaca
     2761 cgcttcctta aagggatgga gacaggggag gggtctcccc ctccccgtca gccccacccc
     2821 ccatggcgat agccaccacg gtccacggca tgaggacagg cccgagaaag aaaggtcgtc
     2881 ccgggnncac ggcatgagga catgcttgag aagaaaaagg tcgaactcag ggacgagccg
     2941 agttcggcat caatctcgtc gggggagaat gacagccgaa atagggcttc accagagcct
     3001 gattcgggaa atcttctccc caggttattt tttgtcatga acgcgggttg gtgcgcatcg
     3061 taattcgatc ggattctctg agatcaattc taccgaagaa ggatctgctc cgctcgagtc
     3121 ggaaaaatga aagaaaatga cgcccaggac ggaattcgaa tccgtgtcga tggcgtgaca
     3181 ggccatcatg ataggccact acactacctg ggcacttttt gttgagggat cccgcctccc
     3241 ggattcgaac cggggacatc gcggtagccg cgcagttcgc ccgagagcga tgactatact
     3301 acagccgcgc actctaccag tctgagttaa ggcgggtctc tgctctaata atgttagcgg
     3361 gagttgttat taaatatttc cctaaatcct cccggctccg cacgacacgg atgttattct
     3421 ttaaatacta ccccggataa tggattggta tgaaccctac aaacgctgag gcacccagga
     3481 acgctgaagc gtactgtctt cttcgccgat agccgtgcga aaagtaacgt cactgttttc
     3541 gacaccacac tgcgcgacgg tgaacaaaca ccgggcatct cattcacgct ggaagagaaa
     3601 ctcggtattg cagagcagct ctccgcgata ggagtgcacg ccatcgaagc gggctttccc
     3661 gcgtcatccg atgccgaacg cgagagcgtc agggccatca gaggtctcgc gctcgacgcg
     3721 caggtctgcg gcctcgcaag gtcgctgcgg gccgatgtgg atgtctgcat cgactgcgac
     3781 gtcgatatgg tccatgtctt catcccgacc tccgacgtcc agcgcgagta caccatcaaa
     3841 aagacccgcc agcaggtcct cgaggccacc ggcgatatca tcgcgtatac ccgcgaccat
     3901 atcggccagt gcatgttctc cgccatggac gccaccagga ccgacccgga ttacctgata
     3961 gaggtgtacc gcgttgcagt ggacgccggg gccacgatca tcaacgtgcc cgacacggtc
     4021 ggtgtgatca ccccgacggc catgaaaaac ctcatcgagc ggatcgcccg ggaggtggac
     4081 tgcccgatcg acgtccactg ccacaacgac ttcgggcttg cggtggcaaa caccatcgca
     4141 gcggtcgagg ccggcgcctc tcaggtgcag gtgacggtga acggcatcgg cgaacgtgca
     4201 ggaaacgccg atctcgcgca gaccgtgatg gcattatcgt ccatctacgg gatcgatacc
     4261 ggcatccgga cgacgagcct cgtggagacc tccaggctcg tctcgcgcta cgcagggatg
     4321 agcataccct ccacgcagcc gatcgtcggc gagaacgcct tcgcccacga gagcgggatc
     4381 cactcccacg gcgtgatcac caggtcggat acgttcgagc ccgggatcat gacgccggag
     4441 atggtcggtc accggcgcag gctgaaactc ggcaagcacg ccggcaggca tgcggtcagg
     4501 cagatgctcg ccgaggtgca tatggagccc ggcgatgccc agctcgacga gatcgtcgtg
     4561 cgggtgaagg agattgccgg caagggcaag cgggtgacgg atgcggatct ctacgagatc
     4621 gccggaagcg tcatgcagct cgcgcccgac gaaaagatcc tgcaactcca ggatgtcgcc
     4681 attatgaccg gcaaccatgt gctcccgaca gccagcgtca gggcgatcat cgacggaaaa
     4741 gagcggatct tctcgagcgt cgggaacggg ccggtggatg cggccgtgcg agcgatcctc
     4801 ggcatcatcc cggccccggt tcacttgaaa gagttcaaca tcgaggcgat ctccgggggc
     4861 accgacgctc tcgggcacgt caccatcgcc gtcgaggatg aaaagggccg ggtcttcgat
     4921 gccagcgcct cgagcggcga catcatcctc gcatcggtcg aggcggtggt caacgcgatc
     4981 aacctcgtct gccggacacg aaagaatgac agcgagcggg aggagtgagg aactcactcc
     5041 ccgtctccag aaaagaattt caggcggcgc cgggaggctg ctgtttgcct tccagcgctc
     5101 ccctgatctg tgaggataac tgctcgaacc tgctctgcag ggccttctcc tgcttatcga
     5161 gcgacttaac ccggagatcg agcgtctcga cgcggtcgtt taaggaagca agcacctgct
     5221 ccttgccctt ctgcatcatg atgctgccga cgttcatgaa gaccgccgca tcctcgggga
     5281 catcggccag atcctcgacc gcccgctttg cttcacggat cgtcatttcg tactgtgctt
     5341 tctgcgagac cacagtctgc agctgctgct gcatctgctg gagcatcgcc agctggttct
     5401 gtactttcgg tgggatattt tccatattca tcaaccctcc tgaaatagga cgaagatcgg
     5461 taactatatt tcgggtggcg gacaggggcc ttcccggctc tctgcccttt ctctacatgg
     5521 taggggcgtg gagcccataa aatgtgaggg ttctcggcag agatcttcgt cgcgtccatt
     5581 cccgggccgc atgcccccgc gagcccgtca cgtgcgcaag gccggggcgg cgatctcccg
     5641 catctcgacg gctatggtga tcagccgcag ccaggtgttg agggccgccc ggagcgcggg
     5701 tatatcggtg gcagtcacct cgagaacgag cgtatcgtca cctgcgaggc gtacacgggc
     5761 ggacgaccgg tcccccacgt cgcccatctc ctgacagaca gagaagtaaa gggcgcgggc
     5821 atcgtcggca gtgaaccgga agactgccgt atgtttcacc ggacaacctt cagggtgtag
     5881 cgcttccgtt gcgtcccgtc aaagacgata cggtgttccg ggagggtctc gccggcaaac
     5941 acgacgtcga tttggtcctt gagcgcctcg tatatgaagc ggtccgagac gaagaccccg
     6001 cgcgcgatca cgcccggacg ctcttcgaca ttgaaggact taaccgcgat ctccaccagc
     6061 ggctctccgc tcgcaaaagc ctggatgaga aaaagagggc cggatttgga gacgaccaga
     6121 accgtctcgt cgagggagaa gaggtcgccc atgcccgcct tgccacgggt gacatactcc
     6181 ccgccgatcg agaacgccag atcccgggag agggagcgca cctcgggaac cggtttacgt
     6241 gacgtcgtga cgaccgtcat cgagccttca gatcttttat tccggctccg cgttccttga
     6301 agaggatgcg gtgtccacag tagggacagc gtatattgac gtcgatttct actttctgtt
     6361 tacaacgagc gcatttatag gcagccacga ctgattaacc ctccttctgc agcgagcgct
     6421 cgatactcct tgtagcgacg cgcatcgccg gtgtctccgg aacgtagctc ccgcctgcaa
     6481 atttaaatcc acacttgcgg cacgcccaga ttcccgttcc ctcacgcttg actgcgacct
     6541 gatcgcagcg ggggcagggg tggcttgcgc gggagacctg ctcgatctgg tttattctct
     6601 tccggataaa ccggccatac ctcgggccca tacgcccggc acttccgact accctgccct
     6661 tagcactctg tttgcggctt gccatttcgc ttacctccaa tacgtcctct aatttttgtg
     6721 ttgtcgatta atatagttaa tcacgacatg cgttcgatga tccgcgtctc tgcatttccc
     6781 cgggagagtt tgttgaccag atcgtagaac tcgtcctgga tgcccgccgg aattctggcg
     6841 acggcaatca gggaaccatc cttctgccac tcactctgct cgagcgtcac cgcggtctgg
     6901 agttccccat aggccctcgc ggcgtaatcc gacgggatct tcacggcgat gcggacttcc
     6961 tcgaaccgga tggggagaag cggccggagt gctttgacca cctctttgac ctgctcctcg
     7021 acggacttga aggggtcgat gctcaccctc gcctcttcca tggcaagttc gatgcgctgc
     7081 ggggggtgag ggaacccgga ctgcgggttg acggcattcc tcgcgatgaa cgtgatgacc
     7141 cggttccgct tctctgcaat aagatggcgg cgctgttcgg aggtgagatg gatctcgccc
     7201 ttcttgatga tccggagcgc agccggttcg aactcggtcg tcttgaagac ctttacgagg
     7261 gactcgtcgg aggcccgttc ggcgtgggcg gagttcgaga agatggtgtc ggctgcaacc
     7321 gcatcctcga tatcgatctc ctccccctgc cggatacgca tggcctgatc ggggtcgacg
     7381 agcacttcga accgttctcc gtagctctca agccgggcta cgactgcctg atccaggggt
     7441 atcatagaag gtaacgctca ctcctcgaac tggtcgacgt aactcttcac ttcgtccttc
     7501 tccagcttcc ggaactcctg gttcgcgatg gagacaacgc cgatctcgat ggcgctcacg
     7561 tcaagcttgc cctctgtcgc ggcatgcagg gccttgatac cgagtacgat ggcgccggcg
     7621 aagtccgcct cctcccggta ctcgtcctcg aagaccttca taaccgcagg ccggcctgtg
     7681 ccgatcccgg tcgccttgta ctcgagcagc gtaccgctcg gatcggtctc gaacagccgg
     7741 gcctccccgt cgctgactcc tgcgatcagg agagcagtcc cgtaggggcg cgcaccgccg
     7801 aactgggtgt aggtctgcat gtggtcgcag agtttcttgg ccagggtctc gacgttgatc
     7861 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc accgcttctc tggcgtactc
     7921 gacctggtag agccttccgt ccgggctgaa gactgtgatc gcccggtcat atcccatttg
     7981 atattgtggt tgcattctcg ttcctccaaa tcgacttcgg tgaaaaacaa tgacttctga
     8041 tgcttaatac ctttttcgac caaatcaacc ttttgacggg gatagcggta caccgtgaaa
     8101 taggtttccc cgaacttcac ttccccggcc tcggagagag gccggatcgg tttcatccgg
     8161 cgccgcagtg catgaatggt cccggaggtg gcgactgtcc ggagtgcggc ccgctcatcg
     8221 gcgatcgcgt tgaccgttgc aagagcggtg gcgatatcct tctctgttcc ccgccggcac
     8281 cggaccacga ggtaccctcc gtcgcagaag accaccgccg gctgcgcaaa accgatagct
     8341 gcatcgcccc agagggaggt cgcggcctcg atgaccgaga ggtagacctg tttctggtcc
     8401 cacgccaccc cgtacgggag aatccgtgcc aggatgtaac gccgctttgc ccgcatcgcc
     8461 gggggcctcg gcttcatctg accaccttca caggctggtc cggctgggtg atccggtcga
     8521 tggacgagag agcctcactc acttcctccc cggtcatacc gaagagtgcg cagagacccc
     8581 ggacgtcgcg gactgaccgc tgctcaagga tcgagcgtgc acccgacgat atcgtcaggg
     8641 gaaagccgta gcgccgctgc agcgagagga tgtccgtata ccgctgcagt gccctctggc
     8701 gcttcgtccc gcggagctgg atgatagggg ccatcgagat atctactgcc acgttatgct
     8761 ccgccgccga ccgtgcggcg acgtggtcga aggcgttcct ccgggcggca tggatgctcc
     8821 tgacgacgtg cacccctttc aacgtaacga tggcccggtt gaaggagata tctcctgcat
     8881 tgacgtagac cacatcggca cgccggacgg tgggctcgcg cacctgcctg atgacctctt
     8941 tttgggatgc agcgctgatc acggcacccc ggaggacctc cggtccggaa ggccgatgcc
     9001 cgacgtctcc gatagcgacg atgctatcga acccgagccc tgctgcctca agcgccatcc
     9061 gcgcaagcgt cgagtcaccg acaggatagg ggtgtatact tgcgtctgtg atcttcatcg
     9121 gagtaaaccg tatacaaaaa aagggttatt tgccgcggtt cgcatgggaa cgtaggctcg
     9181 ggcgtgtctt ctcggttcca cgtccgcgcg tccgcatccc gcgtcccttc atgccggcag
     9241 aggtcttgcc gcgctctgcc cggccccggt gaaccgggtt ggccagccat gcgaggttcc
     9301 ggtcgctccg gaccgacggg tggtgcccat cgaccaggat cacctcaaac cacctggacc
     9361 gtccgtcctc tccaacccag taggagttca atgcctccat gttcgggaac tttcgggacg
     9421 cacgctcttc ggccatgcgc tgcaggctct ttgccggggt catccggcgc atacccatgc
     9481 gggcggacct gcgcccgcgg atgtacctgg gcttccttcg gccaccgcgg cggatcttga
     9541 cccgcacaac gatgattccc tgcttggcct tgaatccgag cgagcgggcc cggtcgatcc
     9601 gggtcgggtg ctcgacacgt acgatactcc cttcgcgccg ccagacctgg agacgctgcc
     9661 agaggaggcc tttcacctca gacctgtcag gccgcttcca tgcctcgcga acgtaggcat
     9721 acattgactt tgccatcttt taatcacctc aggttcagcg tttccgccac attcctatcc
     9781 gggacgtgtc ccgtggttcc taacaggttg gcgagagcgc taataaagga ttcctttcgg
     9841 cggcatgggt ggccgcacga gggcctagac tcccttgcgc ttctccacga catggatggc
     9901 atccatccgg gtgacggggt actgtccgtt ctcgtctttc tctgccgact tgaccatatc
     9961 ccagacggtg aggagagcaa cggagacgcc ggtgagcgct tccatctcga cacccgtcct
    10021 cccgtaggac ttcacgcggg cgatcgcctc gatgtaaccg tcaccctcct cgaaatcgac
    10081 ggtgatgctc ccgagcggta tgggatggca cataggaatg atccgcggcg tgtctttcac
    10141 cgcgagggtg gccgccaccc gtgcggtcgc gagaacgttg cccttgactg cggttccttc
    10201 ccatatggcc cggagcgtct cgctccgcag gtaaattctg ccgctggcaa ccgcttcccg
    10261 gacgacctcg ggctttgccg agatgtccac catctgcgcg cggtcgttct cgatgtgggt
    10321 gaagaccggg gtctcgccgt gtttgccgga ctcgttcatg tgcatcagga gatcttctct
    10381 ttttttgggc ataaaagagg acgtctccgg gcaggtcact ccggtgctcc ccgccggaag
    10441 agttcttccg ggatgtagtc cagcatatcg gtggcgagca taccgttccc tcgcccttcg
    10501 ctcgcccgca tgccggccct gccgctcgcg tacgcagcgg tgcaggctgc ttcgaacgcc
    10561 ggaacctggc agaagagcgc ccccgtgaca ccggcgagca ggtcccccgt tcccccggtg
    10621 gtcatggcag gagtcccggt ccggttgaac ctgacccttc ccccatcgga gacgacgtcg
    10681 accgggcctt tgagcaggat ggtcccccgc gtggcggcag ccttcacgca ccggccgcgc
    10741 gcgatgaggt ccgccggggg ttccgtcccg gtcatccggg cgaactcgcc cgcgtgtggg
    10801 gtgtaaatcg tctctttcgc cgccggcagg gggaggacca gcgcatcggc atcaaagacc
    10861 gccctcttcg ccgccccggc gatggcgagg acgacgtcgt ggctctctct cccgaggccc
    10921 atgccgcaga cgaccacgtc ggcccgcccg acaagccgga ggatcgtctc caggtggtcg
    10981 cccgtgatcg cgttcccctc caggcgctcg tagatcaggt cgggcatggg gatgtacgca
    11041 ggagaggcga tccgcacgat atcggccccg gcacggagcg cccccatcgc cgcgatgtag
    11101 ggcgccccct ggtagggccc gcccccgacg acgaggacct cgccgccggc acccttgtgg
    11161 gcttccgatg cccttgccgg gacgagcaga agatcgccgg ggccggtgta gacctcagcc
    11221 tcgagcggga taccgatatc cacgacgtcc gccccctcca ccttgggccg gtggaacgag
    11281 acgatccggc tcgcccggat gccgggggtg gggatgtcga cggcgacgac cggcgcaggg
    11341 cttccattcg cccgggcaac gagggctgca aacggctccc gcaccgcgcc cgatgctccg
    11401 gtcccgagca tcgcatcgac gatgacgtca gtctcctcaa agatgcggga gagcgcgtcg
    11461 acgtctgagg cgcaccggac cgggtggagg gcgacggagc agtgccggag gagcgatgcc
    11521 tggacggcgg ccgaggcggt catcgaaccg cagtcggggt agacgatgtc gaccgcatca
    11581 aggtgctgga ggtaccgggc cgccaccatc ccgtcgccgc cgttgttccc cctcccgcag
    11641 aggacgagga cgcgggaggg gccataggcc agcacggcat ccgcgagcgc cttgcctgcg
    11701 cactccatca ttgaaagcgt ggagtggccc agcgcgacgg cattctcttc gactgcccgc
    11761 atccgcccgg caccgatcac gccggcctcc agaaactccc gcatatcgtt tgctgccatg
    11821 taccgtctct ccaggagtgt tgtctgatcg gtgctatggc ggtatgatct tagatatctt
    11881 ttgaaaagag ggtgtacgag ggtgcagatg acacctgcat agcgcagtct ttgggcactc
    11941 tcgctccttc acccctggag aggcactcac gccccattta tcgcacaaat ccatgcacgg
    12001 atttccatcg gatatcgcca cgcactgccc ccgcccnngc cgggcggggt gggggttaca
    12061 cgcttcctta agggatggag acaggggagg gggatgctcc tcccccaaag gcgatactcc
    12121 cacggtccag tgtacggggt tcttcatcgt tacagttgct ccggcgcccc gaccacacct
    12181 gcaagcaacc gcaattccaa gactcaagtc acctgaacct taacatccca tttacctaac
    12241 ccatctagac ccttcgaccc ccacactcac atgctttatt gacactcacg acgcactact
    12301 atggagatgg gcctcgaaac agatgtgcgg agagcggcag atactatctg cgatgcggaa
    12361 tcggtgacga tcatctccca catcgacgcc gacggcatca gcacggagtc gatcctctcg
    12421 caggcgctca cccgcgaggg gataccggtc gactcggtct tcgtccgcca gctcgagccg
    12481 atggcgatgc gccatgtccc gaaggacgac tcgctcaaac tcttcaccga cctcggggcc
    12541 ggccagcaga gccttcttga ggaacacggt ctctgtgctg acgaggtcct gatcctcgac
    12601 caccacgtca gccagccctg cgccacggcc tacccccagg tgaactgcct ggattacggg
    12661 tttacccgga tgagcgccgc cggcatcgcc tacctggtcg caaaggcgat cgacccgaca
    12721 ggcaccgacc ttgccaaact cgccgtggtc gggaacgtcg gggatatgat ggcccgggag
    12781 aaccgcgggc tggtcggccc ggcacgcgag atcgtccagg acggcgtgga gtacggcaac
    12841 atcgtcgtgc ataaaccgga cctgaactgc tacgggacgt ccacccgccc ggtccacgtc
    12901 tgcctcggct actgcgacga cccctacatc gacgggatct cgaacagcac gagcggggcg
    12961 ctccggttcc tggaaaaact cggcgttgcg ttaaaggcgc cccggggcgg ctggctcgtc
    13021 tgggaagaac tccccttcga cgacaagcgc acgatcatca gcgcccttgc ccagcagctc
    13081 atcgcgcatg ggagggagac cgaccgcctg ctcgccgaga cctacatctt ccccgacgag
    13141 ccggaacgga cgccgctccg gaacgcctcg gaatacgcca ccctcttgaa cgcctgcggc
    13201 cggtgggcaa agcccagggt cgggggcagc atctgccgcg gggaccgcgg ggacgcctac
    13261 cgcgatgcgg agcacatgct cgcccaccac cggacggtca tccgcgacct gctccagtac
    13321 atcctcgata ccggggtgac cgagttatcc cacctgcagt acatccacac cggcgaccgg
    13381 ttccccgaca ccatcgtcgg gatcggggcg ggcatggcgc tctccaaact gaactggcaa
    13441 aagccgatga tggtgctcgc cgcgatggtg gacgaacccg aggtcaccaa ggtctcgatg
    13501 cggacgaacg agtgggcgct cgcccggggc gtcgacctcc agggggcgct tgtcgaggca
    13561 tcaggcgagg tgggcggcgc aggcggcggg caccggattg cggccggggc atttattccg
    13621 catgatacag aagaggagtt tgttgatagt gttaaccgaa tactcaaacg acagtgcgct
    13681 tcagcggatc cggataatag ctgacttcca gttcggcgcc ggggcaggag ctgcggtatt
    13741 tcccgacgaa tgcacctttc agttctcgac gacgggacgc gtccgccaga ttcgcctcgg
    13801 gaaagagagg cttgccaccg tcagagcaca ggacggccgc ctgaccctcg gggtcgccgg
    13861 agcggctcgt ctcgcggcgg ttcttgcccc cccggcctac cgggtggcgg tgcaggagga
    13921 tgtggccccg ttcattgcgg acggaaagaa cgcgatggcg aaacacatca tcgccgccga
    13981 tgacggcatc cgcgcagggg acgaggtgct ggtggtgacc ggagacgacg tgctcctcgc
    14041 caccggggga gccctgctct ccggccggga gatgctggca tttaattacg gtgtagcagt
    14101 aaaagtacga caggggagag gatcggaatg tttccaggaa agataaaccc gaaaaagatg
    14161 aagcagatga tgaagcagat gggcatggag atggaggaga tcgagggcgt agagaaggtc
    14221 gttatctaca cgcctgcggg aaactacgtc tttgatgatg cccaggtcgt cgcgaccacc
    14281 atgcaggggg ccacaagtta ccagctcacg ggagagcccc ggttcgaaga ggcagtcccg
    14341 gagatccccg acgaagacgt cgccctcgtc gcatcccaga ccggtaaaac ggacgaggtt
    14401 gcgagggcga cgctcatcga gacccgtggc gacatcgccg aagcgatcct gaaactctcg
    14461 cagcaatgat cgaagaaggt gaacgcctcc tgctcgtcag tgaggaccgc gagtacttcg
    14521 tgaaggcagg cgagggaaag ttctccaccg accggggcat gatcgacctc ggagcgctcg
    14581 caggtatgaa ctccggggac gagatacgga cccatctcga cgtcccgttc agggtcctcc
    14641 gcccccggcc gaccgacttc tttgttcatg cgaaacggag cggagcaccc atgctcccga
    14701 aggatatcgg gatcgtgatc gcctacacgg gcatgaatca cgaggaccgt gtccttgatg
    14761 ccggcaccgg gagcggcgtt gccgcaatct acttcggcaa catcgcccgc gaggtgaaga
    14821 cctacgaggt gcgcccggag tttgccagac tcgcggccaa aaacgtcgag aacgcccgcc
    14881 tcaagaacgt cgaggtggtc gcggccgaca tgctcgaggc aaccggcgag ttcgacgtcg
    14941 tccacctgga cctgacgatc acgccggcac acgtggagca cgccttctcg ctcctccggc
    15001 ccggcggcta cctggcatgc tacactccgt tcctcgagca tacgttcgtc gcgctcgatg
    15061 ccgcaacccc gctcttccgc gacgtccact gctacgagtg catggagcgg gaactgacgc
    15121 gctccgcacg gggaacccgc ccgtctaccc gggtcgccca cagcggctac gtcacaatcg
    15181 cacgaaaata aaggggggtt actcccgcag cgggcgctcc cacccggtat agccgcagta
    15241 gatgcagcaa tccccgccgc agtgccggca tttctgcgac ggctgcggca ccatcacggt
    15301 acccttttta ttgcagtaca tacacccaag ccccccgcag tgagaacaga cttcaggggc
    15361 atactctacg ttctcttcgg acatgagttc tcctccagaa ttatatgggg cgggcgaagg
    15421 tgacaaaggt tgttgtgggg gccgggcacg cacccgaaaa aaaggtatta gtgggtgggg
    15481 ggcgaaccac tactcaggag agaagagggg ctttgtccgt gtcatggcgg gaggggcgga
    15541 agattcgggc gcccgccgga cgaaaagccc cggaaggtag ccgcacgatg taccacatta
    15601 tctctatccg cgattttgaa aggagcgatc tcgattatct gctggaccgg gcaggggagt
    15661 tcgataccgg gaagtgtcgg tcccaggcgc ttgatgataa acttgtggcc cttctcttct
    15721 ttgagcccag caccaggaca aggatgtcgt ttgcgacggc catggcccgg ctcggcgggc
    15781 ggtcgatcag cgtggactct gtcgaggcaa gttctatcgt caagggagag acgcttgcag
    15841 ataccatccg ggtggtgagc agttatgtgg atgccatcgt gctccgccac cccaaggaag
    15901 gggcggcacg gctggcaagc gagttcgcct cggtgccggt catcaacgcc ggcgacggcg
    15961 cggggcagca cccgagccag acgctgctcg acctctacac catccggcag tcgatgccga
    16021 tcgacgggac cgatgtcggg ctcctcgggg atctccgcta cggcaggacc gcacactcgc
    16081 tggcgctcgc cctctccctc tacggcgtca ccctgcacac gatcgcgccg ggggggctcg
    16141 agatgccggc gaacataacc ctcgaactcc gggaacgcgg catggaggtg atcgagcatg
    16201 cggacgtcga ggaggcgatc cgggagctcg acgtcctcta cgtcacgagg attcagcgcg
    16261 aacggttccc ggactcggcg tcgtactaca acgtcgcgtc cagttaccgg atcacgcccg
    16321 accttctcgc cggtgtccgg gatcacctga tgatcctcca ccccctcccg cgcgccgggg
    16381 agatcgaccc ggcggtggac cacacgccgt atgcacgcta ctttgagcag gcaaggaacg
    16441 gcgtgcccat caggatggcg cttctccatg aggtgatgaa atgagcacca aagacccatc
    16501 acggggactg ctcgtcagcc ctataaggaa cggcacggtc atcgaccaca tcacggccgg
    16561 cgaggcgttg aacgtcctgc ggatactcgg catcaccggg tcgacccccg aatgcctgag
    16621 catcgccaca aacgtcgaga gcaaacggat ggggaagaag gatatcgtca agatagagaa
    16681 ccgggaactt cgcacggagg aggtcgaccg gatcgccctg cttgcaccgc aggcaaagat
    16741 caacatcatc cgcgactacc gggtggtgga gaagaagggc gtggagatcc cgaagatcct
    16801 caaaggcgtg gtcaggtgcc cgaaccccgg ctgcatcacg aacacgaacg agccggtgga
    16861 gagcacgttc gaggtgctcc ccaaagggct gcactgcctc tactgcgact ggctgatcaa
    16921 ggacgatatc gcaaaccaca tcatctgatc aggcgtccat gccggtcgat ctctccccgg
    16981 tagacccggg tgctcgagat ccgccggccg tcttcggcga ggacgcatga gatctcatgg
    17041 aggtccactt ttttttgccc ccgctcacgc cggaggtcgt tgatctccac ggcaaccggg
    17101 aaggtctcct cggagacgac gaggatgtcg aagtccgcat cgagggcgct gccgtaacgg
    17161 tcggcgagcg gctcgacgtt ccacgccgca gtgtagccgt gctcccggat gaaggacgca
    17221 atgcttgcca gccgttccgg ataggagtgg acggggtgca cctttgcacc ggcaaactca
    17281 tcggtcgtca acccgatgat cacctccccg tcaggcccgg caagctcaaa ggagcgggcg
    17341 aggagtttcc tgtgcccggc atggagggga tcgaacgtcc ccccgaccat cactttcatc
    17401 tggaggcgtt tgccgcgagg ggacttatgg gtagtgatcg gtgagtggga ggggagcggc
    17461 tcagggggtg aaagggcgtg gagtattttt gggagagatg gctgggccgt gtccccctcg
    17521 gttgcgacca gtggaggtca tacgctggaa aagctccata ctgtggaccg tgggagtatc
    17581 gccatagggg gaggggc
//
LOCUS       DAJB01000165           16169 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65100, whole genome shotgun sequence.
ACCESSION   DAJB01000165 DAJB01000000
VERSION     DAJB01000165.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16169)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65100"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3087 a         5044 c         4940 g         3098 t
ORIGIN      
        1 ggatcgcgcc ggagctctac ctcaagcgcc tggtcgtcgg cggtttcgac aaagtcttcg
       61 agattgccaa aaatttcagg aacgaagata tcgacaccaa ccacaacccc gagttctcga
      121 tggtggagat ctacgaggcc taccgggact acaacgacat gatggacctc accgaggaga
      181 tcctctcgca cctcgcggag aaggtgtgcg ggagaaccac cataccgttt gcggggcacg
      241 acctgaactt cacccgcccc tggcgccgtc tctccatgga ggaggcggtg caggagtacg
      301 ccggcatcga tatcaaggcc cataccctgg aagaacttca tgcgttcggc cttgagcact
      361 gcgtcgaggg ctgcgagagc gccgtgaccc ggggcgagta cctggtgctc ttcttcgagc
      421 acttcggcga gaagcagctc atccagccga cattcgtcta cgacttcccc atcgagaact
      481 cgccgcttgc aaagaagcac cgcgagaagg agggaatgac ggagcggttc gaactcttca
      541 tcgccgggat ggagatggca aacggcttct cggagttgaa cgacccctta gaccagaagg
      601 cccggctgga actgcaggac gcaaaacgcc ggcggggaga ccttgaagcg cagatgatcg
      661 actacgactt catcaacgcg ctcggctacg ggatgccccc caccggcggc gtcggcatcg
      721 gcatcgaccg tctggtgatg ctcctcaccg gcaaggactc gatcaaagag gtgctcctct
      781 tcccgcagat gaagaccgcc gtcccgggac cgaacgggtc cgaaacggag cagaccggcg
      841 gggaggagtg atcatccccg gtttttcgcc atgaacggac agaaccgggc tgccatccgt
      901 cccggcatga cggtggatgt cgtgctcaaa cgtgaccagc gaaccgggaa actcacccgg
      961 ggcgttgttg ccgaaatcct gacgaactcg tccttccacc cccatgggat caaggtctgg
     1021 ctccaggacg gacaggtcgg gcgggtgcag gtgatcgtcg ggtgaaaccc atcggcttca
     1081 cccggccttc cggagggtga accggattgc cgcgccgcac tccggccggc ccgctacccg
     1141 gtcatccgcc cggatactgc cgccgtaacg cgatacaagc atccggcaga tataaagccc
     1201 gaggccctgg ccgctctttt cgccgtcccc accgctgaag cggttgaaca ggcgttgttt
     1261 cctctcgtcg gggatacccg gcccgtcgtc ctcgaccgag acctcgacct cctccccgtg
     1321 ctcctcgacc cggacggcga tgcacccccc gggaccggca aacttctccg cgttgccgat
     1381 gaggttcgtg aagacctcgg agaggaggtc gtcggcgagg actgcgaccg gatggccctc
     1441 gtaccggatc ctgatcgcgg ggtgattcgc tacctgtgtc cggataatcc gatcgagatc
     1501 cacccgcgtc aacgctgctc cgctctcccg ggcctcccgg agggtggcga tggagccgat
     1561 gatcccggag ctctgctcga gccgggagag catcttcctg gcctcttcct gccgctctcc
     1621 ctcgagagca tcgatgagaa actgagtata tcctatagca atggcgttcg cattgttgat
     1681 gtcgtggacc atgatgtcca ggtagagcgc ggccgcctgg ctggactctt cgagatcggc
     1741 cgtccgctcc cggaccagat cgctaagccg ggtgctgtac tctcgtgtca tctcctctga
     1801 ggcgcggagg cgctcgatat tccctttgag agagtcgatc atggccccga tgctcttctc
     1861 aagacgggta aattccgctc ctgcagatgt ccggatcggg tgttcgaggt ccccccgggc
     1921 gatggcgttg acatcgtcga cgatctcctg taccggccgg gtgatccgcc gcgaggccgg
     1981 gaacgcgatg cagcaggcga tcaggctcgc gaagatcgcg acgagaacat gggtaaacca
     2041 catctccgtg agccgggcat caagaggagc gggggtgtag atcagctcga caacccggct
     2101 cgtgtccgag ggatagtcgg gggtggagag gtcgacaaaa atataccggg tcaatcgtcc
     2161 cgttgccgga tccgtcatcg tccgaccctc tttctcctca aatactgcca gggcaacgga
     2221 attgataaca gtgggatcgg tcgggccctc cgaatcggtc acgttgacga gcctcccata
     2281 acagtcgaag atccgtatct cttcgagtgc cgggttcaac cgcatcaggt cttctttgag
     2341 cgtctggtat tgtggatcaa accgatcagt ctcaatcgca ctgcagatga gccccaattc
     2401 gaagaggtag cggtgatcag gggagggcat gtaagcgtat ttccgcaacc gtccgctcgc
     2461 cggctccgtc aggattcggt ccgcgacaaa cgcatcgccg agccggatct ccgttatccg
     2521 gtcgtagaag gaggggaacc ccctgaaatc gaggccgagg tccggagggt aggtggtgta
     2581 ctcgatggcg cctgacgcgt tgatgatgta gaggtcgata tcccccccga tctccttctg
     2641 gacgcgggag aggtccatgg cacccgggtc ccttcccgcc cgctcgtatt ccgcgatgaa
     2701 cgggccgaac ccctcctgca tctcgctgtt taaggtgtta tcgaagagtt tcatgcctgc
     2761 atccacaagg tgtatcgact gcatgacgct cctctccgtc tgctcccgga gcagaccatc
     2821 ttcggcgatc agagctccct cgaccacctg gtagtccagt gccgagatca gcccgatcac
     2881 cgggagcgtg accaggagca tgacgagcac cagatgtgtg ccaaagcggt gaggcctctt
     2941 gccgccatcg ccggtcaccg tcatgggctg cctccctctc cggccttccg aagcgagaag
     3001 cggaaggctg cgccgcactc cggccggccc gctacccggt cgtccgccca gatcctgccg
     3061 ccgtaacgct cgatgagcat ccggcagatg tagagcccga gcccggtccc cgcggccctt
     3121 tgagttcccc gggccagccg gccgaagagc cgggacttta tagcatccgg gatccccggc
     3181 ccggtatcct cgaccgagac cagcacctcg ccgtcctgct cttcgacccg gacggtgatc
     3241 ccgacccccg gtccgccgaa cttcgccgcg ttcccgatga ggttcacaag gacctcggag
     3301 aggaggtcgt cggcgaggac ggttgccgtg gtgccggcat aggttatcct tgcgagagga
     3361 gaacgctcta tctcgcgtct tacgagcggg tcaagggcta tcggctcaag acggcgtgct
     3421 cccccctgga tatgctggat ggtgttgacg ttgtgcacga tctcgatact ctttgcaagc
     3481 cccatccttg ccttcctgag caactcagcc tcgggctctc cctcaaggtc ggcgaggagg
     3541 aggtctgcgt agaggttcgc gacgttgttc gcgttgttga tgtcgtgccc cataatatcc
     3601 agatagagtg ccgccatccg gttggactcc tcaaggtcgg cagtccgttc acggacctgt
     3661 tcttcgagga catggctgtg ccggatgatc tcctcctccg actcccggag cttccacatc
     3721 gtctctttta aggatgcgac catcgccgat atgctcttcc cgagatgcac gaactcctcg
     3781 tccccgctca cccgtatggg gtggtccagg tcaccgtggg cgacggcatc gacgtcctcg
     3841 acgaggatgc gtatcggtcg cgtcagggag tgtgcaacga gagccgagag gcagccgatg
     3901 cagaggaacg cgacgagcag gatgctcgca tgttttccga gcatcccggc aagttttgcc
     3961 tccgcggcct tcgtggtgta ggtcagttcg acgaccatgc tcatatccga tgcgtactcg
     4021 ctgtccggca tatcgatgaa gaggtaccgg gtcagttcgc ctgcagtggc gttctccacc
     4081 tcaagtgtgg tcttctcccg gtatgcctgc cggaccatgg ccagccgggc gtcatcgtcg
     4141 ggatgcttct tgccggtgat ccgctcaccc aggcagttga agatccggat ctccagaatg
     4201 tccggattta tgtccaccag ctcccggaca gtctcccggt acttcagcgc cgtgcgatat
     4261 tgccgaaact cagaccccgc aaggccgagt tcgaagaggt aacggtgatc gggagagggc
     4321 atgtaggcat acttccggag ctctccggtg gagatctcgg caacgaccct gtcagccagg
     4381 aaggcgtcac cgagccggat ctcggtgatc cggtcgtaga aagaaggtat cccggtaaag
     4441 tcgtacccca gttcgggagg ataggtggtg tactcgatga caccgctcgt gttgatgatg
     4501 taaaggtcca tcctgccccc gagctgctcc ttgacccgga ggaggtccat ggcgcccggg
     4561 tccctccccg cccgctcgta ttccgcgata aacggcccga acccctcctg catctcgtgg
     4621 tttaaggtgt tgtcgaagag ctttaacccc gtgtccacca ggttcatcga gagggcgatc
     4681 ccggactcgg tctgatcccg gaagtcttcg gcgttcattt ccagttcctg tctgactccc
     4741 atagaatcga cgagcgagag caggcagacg acgggggcca tcacgagcag gatggcaagc
     4801 aggaggtaga gcatgaacgg gagcaacggt cgcaccgggg tctttgccgc catttacata
     4861 tacactccgc tcttcatgca ttactttccc cgggattgga acagttcctc caagagggat
     4921 atatccgtag ctatatagca tgaacgattt tgacacgaaa gggcagattt agacaacgct
     4981 tttaggcaag gggccccgga gatcgaagca aagcgcactc aaaaccgcat tgagggaaga
     5041 gttgcacggt agcgggcacg ggatagaaaa agagaatacc gggaccggat tgcggccccg
     5101 gatcgtatcg tgggacggta cggtgaactt acataggggg catgcccatg ccacccatgc
     5161 cgcccatctc gtccatgccc gggccacctg cgggtccggc agacttggat gaggcgatga
     5221 cgtcgtcgat ccggaggatc atgacggcgg cttcggcagc gctggagatt gcctgggtct
     5281 tcacccgcag aggttcgatg acgccggcct cgagcatatt gccggatgcg ccgttgaaga
     5341 catcgagtcc catgtacttt ccgttcttac cacccttctc gtgggctgcg cggagagcga
     5401 cgagcatgtc gatagggtcg aggcctgcgt tctcggcaag ggtcctcggg atgatctcaa
     5461 gcgcgtttgc gaatgcctcg atggcaagct gggcgcgtcc gccgacactt gcggcgtact
     5521 cgcggagacg cagcgagagc tcgatctcgg gcgcaccgcc gccggcgacg aacttcttgt
     5581 cctcgatggc aacactgacg accctgagag cgtcctcgat ggcacgatcg agttcggcaa
     5641 cgacgtgctc ggtgccgccg cggatgatga tcgagacggc tttggggttc tcgcattcgg
     5701 tgacgaagat catctcttcg ccggagacct tcttctcctc gacgctgcct gccttaccga
     5761 gttcctcggc ggcgatggcg tcgatggagc tgacgacgga accgctcgtg gcgcgggcga
     5821 gcttctccat gtcgctcttc ttgacacgcc gcacggcaaa gatgccggcc ttggcgaggt
     5881 agtgctgggc gatgtcatcg atgcccttct ggcagaagac gacgtttgca ccgcttgcaa
     5941 taaccttttc gacgatgccc ttgatcatcc gctcctcttc atcgaggaac atctggagct
     6001 ggtcggggct cgtgatgctg atctcggcgt cgacctcggt cttcttgaac tcgacggcgg
     6061 cgttgaggag caggatcttc gcgttcttga cgacacgggg catggcgggg tgcacacgct
     6121 ccttgtcgat gatcatgcct tcgacgatct cagagtcctc gatggacccg ccgaccttct
     6181 tctcgacctt cacgaaatcg gtgtcgacgg tgccgtcggc gtcagcgacc atcgtgatcg
     6241 ctctgacggc gagttcggtg agtttatcct ttgcgacctc ggcacccttg ccggtcatag
     6301 cggtgtcggc aagcttgcgg agcatcacca tgtcgtcggg cctgatgtcg acggcgatct
     6361 cactgaggat ttcctgtgct ttgtcggcgg ccatgcggta gccgttggcg atgaccgtgg
     6421 ggtgcacgtc ctggtcgagg aggtcctcgg cacgcttcag gagttcgcct gcgatcacca
     6481 ctgcggtcgt ggtgccgtcg ccgacctcat cgtcctgggt cttggcgatc tcgaccatca
     6541 tcttcgcggc ggggtgctcg atatccatct cttttaggat ggtcacaccg tcgttcgtga
     6601 tgacgacgtc accaatggtg tcgacgagca tcttgtccat gcccttgggg ccgagcgttg
     6661 tccttacggc gcctgctacg gcctttgcgg cagcaatgtt gcccgactgt gcgtcacgac
     6721 cgcgggtacg ctggctaccc tctttcagaa tgaagattgg ttgtcctcca aggtttgaca
     6781 taagtatcac cgttgttaag cgatagaaag gtaggtttgt agttctatat aaacctattt
     6841 actcgttcat catatctatg agttccgagc cgtcttcaag ggtgtggaga tgctcttcgc
     6901 cgataaggag ggttttgccg atttttttct gctttttgta atccgtgacg acgcacaccg
     6961 caaacgtctt cgtgatctgg gagatgttgc cgatgagaga agcgcgcttt acaatcttct
     7021 gggaggtccc gtaggccgtc aggatggtat gctgaccaaa gagtgccagc gcctggaacg
     7081 gggcctgccg catagcgtgg atctccattc ccatgcgttc gagatcgatg aggacgtcgc
     7141 cggccgtgga ttcgggaggc ttctcgggct cgggggagcg gtaggcgatg agctcgatcg
     7201 tctcgacgag cggggcgttg aagagttcct caagcctgat ggcaacgtcg agcgtggtac
     7261 ccatcccgct ctcgtacttg ctgatggtcc ggcgggagac cccgagacgc gacgccaggt
     7321 ccccgagcga catccgggag cgctcccgca cctcccgcag gaggtcgcct ttgatcttca
     7381 cgtagaggcc gccgggggat gcgtagacca tcggcgagag cccttccacg aagtagtcgt
     7441 agagtgtctc ggggctgagg gcaaagagtc cgtagcggat gtagacgacg ccgcgttcga
     7501 ggtccgcatc gcgagcccgc tctccgacga tgagaggggt ggcctgaagg taccgggcga
     7561 tctggttgag gtcccaggcg atatcggcac tcacgctgtc gatgtgagag gcgactttga
     7621 tgatgaccag gctgtcgcct ttcttcgcga taaggtcgaa actccgcgga cggatgttgc
     7681 accgctccga gacgtcgaag tccgcgagaa gcatgatgct gatgaccatc tgaggaaggc
     7741 gatcctgcga cataacccgt aagaaccgat atagccggag aacgatataa actagagggt
     7801 tatgtggatc gggatcgacg acacggattc ccccgcaggg atgtgcacca cctatatcgg
     7861 ggcggttctg gtgcggcagc tcgcgcgatc aggattccgt gtcgtcgaag cccggctggt
     7921 caggctgaac ccgaacgtca tccacaagac ccgggggaac gcggcgatct gcatcgaggc
     7981 cgagggggag atcgaggcgg tcttcgcact cacctgcgcg tgcgtggaag cgctcgcgga
     8041 gttcgatgct ccgggaacca acccgggcgt ggtggtctcc cgcgtccggc caccgccgga
     8101 cttctactac gcggcgctcc gggacttctg caccgtggag gaggccgtcg aggtgctcga
     8161 ggcagccggg gcccggtacc ggggctacaa gaaccggcgc ggcctcatcg gcgcaacggc
     8221 ggcggttgcg agcgatctgc ccgaccggac ctacgaactg ctcgcctacc ggacgcggga
     8281 gcgctggggc accccccggc aggtcgaccg ttcgagcctc ttttgcgccg aggagatgac
     8341 cggaccccac acctgggaca cagtggaccg ggagaacgat gtggtggtct gcgtgcccca
     8401 tacccccgac ccggtcctct tcggcatccg gggggagagc cccgcctggg tcggcagagc
     8461 ccggtcctct gtccgctcgg aggggccggc ctgcgaacag gtctacacca ccaatcaggg
     8521 gacggacgct cacctggtcc cggggcagat cggagaactc cgggaggggc ggtcgtacct
     8581 ggtgcggggc acggtcgcgg gctccccgac caccggaccc ggcggccatg tctcgtttct
     8641 cctcgcagat gaaggcgttg aggcccgctg tatggcctac gagccgacca aagggttccg
     8701 ggacgtcgtg aggagactcg tccctcacga cgtggtggtc gcggccggaa gttacaaagg
     8761 cgggagcctc aacctcgaga aactcggggt ctgccacctc gccgatctga tccggatccg
     8821 cccgccggtc tgcccggcct gtggaaagcg gatgacgagc gccgggacag ggaagggcta
     8881 caagtgcagg gtctgtgggg cccgcagccg cgagcccgag gctgagcggg tcgaacgcct
     8941 gctccagccc gggtggtacg aggttccccc caccgcccgc cggcacctcg cccggccgct
     9001 ggtgcgtggc gtccccgcgt gggaggaagc ccttcttcaa tcaggccgaa aatgtagccg
     9061 ccttccccac cggcagccat gacctgcgcg ggtggggagc cgggaggctt tcccaccccg
     9121 tcaatcaaat gaactttaca aaaaaagatt tataatgttt gtgtgttaac ttatccctgg
     9181 tgatcccgtg ccgactatca gcgagagcgt acgtcctctg gcctgtccgc ccgcaacggt
     9241 gccaaccccg ggcagacagg gttcacctgg cggccgtacg gactcgcggt cgatatgcac
     9301 ggaacgaagg taaccctcag gataaccttc tgtttccgac cagatagtca gctcaggaga
     9361 gatggcccgg acataccgga ccaccgacac ggcgaaacga gcgggatcat cccgtgtcga
     9421 acaatcaatc agtctctgtc tggcagcggg ggacccggcc accgggctcc cgccactgtt
     9481 ctgtcgttat cagacgcctc cccggcccgg tgggcgtcag gttcggtatg acatccggta
     9541 gtcagggcgg ggtttcccgc gggtacggtg tcctcccagg ggttttcaag cactgaggga
     9601 tacgaggggc gtcgggcacc gcaggcgtac cctgtgacag cctcacgaaa agacgatcca
     9661 ttatcgtgca tgaataccaa ccctctccag agagctggtg taacactatc atgaccttca
     9721 aatatgggga tgcggtacaa caggcacggg tgcgccccgg gatgacggtc ggtgaactcg
     9781 tcgatgaact cgggaaagcc ggagcctaca acggggggtc cctctggcag gcggtcaaca
     9841 tctacgagcg gatgctccgc gatgagaagg cgctgaagtt cttcggcctc tcgggcgcca
     9901 tggtgcccgg cggcatgggc ggcatcgtcg ccgacctcat ccagcgccga cacatcgacg
     9961 tactcgtctc gacgggggcg aacctcaccc acgacgtcat cgaggcgata ggctgccacc
    10021 actaccatgg aacctgccag gtctccgatg ccgaactctg cgaggagggg gtcaaccgga
    10081 tctacgacat cttcctcccg aacgatgcgt tcatcagatt cgaagagttc atgcaggacg
    10141 tctactcgtc cctcccggag ggctcgacag tctcgatctc cggcctcctc aaccagatcg
    10201 ggagccggct tgactccggg atccttgccg gggcagcaaa ggcaggggtg ccggtctact
    10261 gccccgccat ccaggactcg atgatcgggc tgcagtactg gctttttgcc cagacgcaca
    10321 aggtcacggt cagcgcgttc ggggacatgc ccgctctcct cgaccggtgc tttgaagccg
    10381 aacgggccgg gaccgtcctc gtcggcggcg gtgtcccgaa gaactacatc ctgcagagca
    10441 aactgatgac cgagagcggg tttgactacg cggtgcagct caccggcgac cggccggacc
    10501 tcggcggcct ctcgggcgcg acgctcgatg aagcccgctc atggggcaag ctcaccggcg
    10561 aggcgaccgc cgcaaccgtc tacggcgacg cgaccatcaa cctgccgctc ctcgtggccg
    10621 caaccctcga taggctggaa cgatgaccga acttatcctc tccctggacg tgctcgaccg
    10681 gaaacgggcg gtgaagatcg cggagtcctg tgcgcccttc atcgacgcga tcaaggtcgg
    10741 ctatcccctt gtcctctcgg ccggcctctc tatcgtcgag gacttcgccg ggctcggcct
    10801 gccgctcatc gccgacttca aggtcgcaga catccccaac accaaccgcc ttatctgtga
    10861 agcggtattc tcagccgggt tcgatgccgt gatcgcccat gggttcgtgg gagccgatgc
    10921 cgcgaggacc tgcgtcgagg cggcgcacaa ccacagcggg gctgcatata ttgtcgccga
    10981 gatgagccat cccggggcga ccgagttctt ccatggtggt gtggccgaac gcctcgcgac
    11041 gctcgccgtc gcctgccggg ccgacggcat catcgcccct gccacccgtc cggaacgggt
    11101 cgcccggctc cgcgagatcg tcggcaagaa ggcgatctac tcccccggcg tgggagcaca
    11161 gggcggtgac ctcgacacgg tggcccggct ggtcgacggg gtcatcgtgg gcaggagcat
    11221 ctacgaggca gcggaccctg cagctgaagc cgaacgcctc tcacgcatcc gccggtgatg
    11281 agttctccgt tccgatcctt tgggagatga cgaaccctat gagtggtctg aggcggatgc
    11341 ggctcatccc gcatctgaag ggttatatat tctcccgcag atactcgtat catgaactgg
    11401 agccccgagc agcagaaact ggcagaaaaa taccggagcc tcgacgaaat ccccgcaggc
    11461 gagcggcgat acaagtgcca cacctgtcac ttcgtcgtgg acgagacccc ctgccctcac
    11521 tgcggtgaag cgtcgctcga ggtcatgtgc ccgcttgacc actgcgactg ccaccactcc
    11581 atcgtcgaga agatcgagta ctgtccgctc tgcggccatg ccgtctgccc ggagtgcggg
    11641 agccatgacg tcgtgcagat cagcagggtc accggatacc tccaggacgt tgcaggctgg
    11701 aacgccggca aacaacagga actgaaagac cgggtccatt actccgtcgt atgagatatt
    11761 ttgtcaggga cgatactctt ttccttcgcg gccggttcag ggcggccagc accggcatca
    11821 acggtggtat cgccgatgtc acgacggtat taaaccacac ggtgccgcac gatttcaacg
    11881 acgacccgcc acgccgcctc gatcttctcg ctgcccggca cggcctcttc aacgagtact
    11941 tcgggctcct gaccgccgtc gagatgcatc acctctgcgt gctccagtat gacttcatca
    12001 cggtcttcat cacggcgggg gtgaccaacc cgaccccgtc ccccgctccg cataccatca
    12061 acatcatcgt ctacagccgg gaggggatgg gcgacgcggc gctcctcgag acgatcgtca
    12121 ccgcaaccgg ggcgaaggcc caggcgctcc acgacctcgg ctatgatttt cccgggacca
    12181 ccacggatgc ggtcgccgtc gcctgcgagc gcgatgccgc cccggcacat acctacaccg
    12241 ggacgctgac cgaagccggc cgacgggtcc atgcggccgt cctgcacggc gtcccggagg
    12301 ccctcgcgcg gcagcagggc agggtgcggc ggagcgaccc gtcgttcttc atctacagcc
    12361 gctacggcgg ggatcactgg gtggagtggc aggaagaggg ctgtccctac tacccctgcc
    12421 acttcccggg ccagcggtgc gactactgct actgtccctg ctacccctgc accgacgagg
    12481 acctcgggga atgggtcgag agtttgagcg gcggcaggat ctgggggtgc gccgactgta
    12541 cgctcctcca cgtcccggag gtcgcaaact acatgaaaag aaatcccgag gccaccctcg
    12601 ctgagctcaa gcgcctccgg gaccggttat aagatcgtta ctgcttcacg cttctgcgac
    12661 gccgagggcg gagagcatct cttcgagggg aacaccgtgt gcctgggcgg cttcccggat
    12721 cgtctcgttg tttgcgatgg cacagccaag gcaacccatt ccaaaccgga agagtacctg
    12781 tgccgattcg ggcttctccc gaaggaggtc cgcgattgta ctgtccgcat tcaatatcat
    12841 acagctgatg ttgtccctgc ccctatatat acagttaagg agtcggtgca gaggtgccct
    12901 gatccaaccc cggactgcaa aatgacgcat ccccccgccc ctgcgggtgt gggcaacccg
    12961 cgaggaccca tagaccccat ccccggcgag gagatgcttt ccgccccacg gagcagacgc
    13021 gtggcagaaa acttgggaca gccggctttt gccgcgcttt cccccggccg ggcgcatcgg
    13081 ggactcccgg cgggaagaac cgggttcatt ttcacaccgc acgccgagtc ttaatagcct
    13141 gatgagacaa agtacagact ggagatgtaa gtatgaacct atctgaggta acagagagaa
    13201 tctcccggaa acttgaagca aatggtgcac agcccgatcg gcagaagatc gaatcacgcc
    13261 ttcaccgtct cgttgaagag ttcggcgtca acaccgccga ggccgagcgg acggtgacgg
    13321 ctgatctcgc ccgtgagtat aatctcaccg gtgtcgggag cgctggaaac acctcaacgg
    13381 agctccaccc gataagcgag atcgttcccg gtgactgggt tacgatcgag ggaaagattg
    13441 tcgccctgac cccggcgctt tcaccctcga tcgcgcagaa cgggatcgtc gccgactcga
    13501 gcggcgccat acgcttcgtg acctggaccc gggcgaacgc ccctgcgatg gagtacggcc
    13561 actggtaccg gatcgagtca gcggtcgtcg acgagtacaa aggcgcgccg aacctgaaga
    13621 tccactcggg aaccaccatc tcccggatgg agaaggatgc tccgctcctc ccctcgatca
    13681 caccggttgc cgagctgaag ccgggtgtgg ggagcgtgcg ggccaaggtc atccaggatt
    13741 gggaagtctc ccacgagcgg atgctccaga cggggctcct cggcgacgag accgggatca
    13801 tcaagttcgt catctggaag gaggatacga aggagaaact ggaactcgat gctgtctaca
    13861 acatcttcta cgccaccgtc gacgagtaca acagcaggct ctccctctcc ctgaacaccg
    13921 cgatgtacat atcggatgag ggcgatatcg aggtcggacg aaacgagatc gagatccagg
    13981 gagctctcgt ccatgtcgct cccggctcgg gcctgatcaa gcgctgccct gtcgagggct
    14041 gcaaccggac cctctcacgg cagaactact gcccggtgca cgagatccag cccaacttcc
    14101 gctacgacct ccgcttaaaa gcggttctgg atgatggcat acgcgcccac aatgtcctga
    14161 tgcagcggga gatcgtcgag aaacttgcag ggatcaccct cgaggaggcc atcgggatcg
    14221 cggagacgaa cccgcttggc atggacgaga tcttctaccg catcaggaac gcggcgctcg
    14281 ggcgctacta cacctgcagg gggagcgagt tcggcggcag gctgctcgtc aattcctgca
    14341 ccccggtaac gttcaagccc gaggaactgg ctgcattgct gaaccgcgcc gggggtgagg
    14401 cagcatgaaa ccccagagcg cattcgaacg tgagccggca cgcagggtct tcgcatccga
    14461 actccgggag acccgctacc agttcaagga gggcgaggac gagaagagtc cgacctatgt
    14521 cctcctcccg agcggcatcc ggagcaaccg gatcttcatc gtcgggaccc tcacggagaa
    14581 gcagcggaaa ggggaccaga acatcttcta ccgtggacgg gtggtcgacc ccacaggcac
    14641 cttcttcatc atggccggat cctaccggcc cgaggcgatg cagcagctcg cccgaatcga
    14701 gccgccggca ttcgtcgccg tcgtcgggaa gccgaacctc tacacgacgc cggacgagac
    14761 ctgcctggtc tcggtcagtg ccgagtcgat cacagtcgtg gaccgcgaga cccgggacct
    14821 ctgggtgctg gataccgccc gcgaaaccct ggaccggctg gaagcgttcg gcaccaccga
    14881 cgactccaga aaggcccagg aggagtacgg aacgcagccg gacatctatc ggaagatcgt
    14941 ctacgacgcg ctcgcccagg tgcagttgta aggcggcgat cttcgcggaa tctctttttt
    15001 ttacggaacc gtcgtttctc cccaaccaaa aagccctgca catcgtcccc cttctcgcag
    15061 caacccggtc cgggccgcag acgggttcca acacggcgta ccagtgcatc tcttcaccgg
    15121 cgacgtactg ccgggcgtgg cgatggggct caccgtatcc ggaatcgact tatgtatgcc
    15181 ggagcgacat cagaagtcag ttccgagggt agagtgatcg acaaaaaaga taattcccga
    15241 tgggggcgag acacccggga gttactccgg catctctccg gcgggctcct gccgggcccc
    15301 cttccactcc cccttcagtt tcgggtagtc cgcgaggatc ttgctgaccg tgctcctgct
    15361 gacgtcgaac atcctgcaga tctgactgat gctcgtcccg gcctgccgta tcgtgaggag
    15421 ggcctcgacc tgaccctcat tgagcgcctt cggcctcccg atgggccgtc cgtcctcttt
    15481 cttcaccttc tttgagacga ggggggcgat tttccgggtg ctctcccggt actggtccag
    15541 atacgcgacg aagttgccga tagcctctct cctctgctcc gggtcgtccc ggaacccgaa
    15601 gaagtcgtta ttcacgcagt aaaccgtgta ttggtcgttt aaggatttga gcgctgcaag
    15661 cccggcatcc agatccctgg caaggccggc aaggtcgtag atgacaacgt cggggcagtt
    15721 attggcggca cagtaatcca gcatctcagc aaagcccttc cgtttctccg gagatgttgc
    15781 gctcgcccgg tggtcgtgga agatttcaac gatctgaaac cgatatttac aatagttctc
    15841 gacttcactc ttttgtgctg cgaaatcccc cttctttctc gtattcaagt atgccacagc
    15901 atctcttttc atccgtttat gatgccgtac aatcatatat attgcttttg aaaaaatacg
    15961 agcaacgcct tattgcagca tgacaggaaa tcccgtgcag attcctgctc tattgccggc
    16021 aatgtgtaaa taaggtctca ggatacatgg tgaactaccc cgccctgaag ggcggggctt
    16081 cctgcttcat ggacaacact tgcatcacag agatgtgatg tagaggcatc gtctccacag
    16141 gctcgaaggg ctgttccatc cccacgcgg
//
LOCUS       DAJB01000166            3015 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_50986, whole genome shotgun sequence.
ACCESSION   DAJB01000166 DAJB01000000
VERSION     DAJB01000166.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_50986"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          598 a          924 c          980 g          513 t
ORIGIN      
        1 ctcttcggcc agccggtccg cctcatcccc accctccacc ccgccgccgc gctctataac
       61 ccggcatacc gggaggccct ggaagaggat ttccggatca tcgggcgcga actcgcagga
      121 gccggagaat tcacgacaca ggactgaagg gccatggcaa aagagcgttc atgtggggcg
      181 gtcgtcgtcc ggcgggatac gggtctccag tacctcatcc tgcagtacgg ggccgggcac
      241 tgggacctgg tgaaggggca cggcatgcgc ggcgagagcg aggaggagac ggtgcttcgc
      301 gaacttgagg aggagaccgg gatcacccgc gcagagttcg tccccgggtt ccgggaggag
      361 atccactact tcttccagcg ccgggcacac acggtctaca aggaggtcgt ctactacctc
      421 atcgagaccc cggaggagga ggtgacgctc tcggacgagc atatcgccta ccagtggctc
      481 ccgcacgacg aggcgttgcg cgtgatcact ttcgggaact caagaagggt cgtcgacggg
      541 gcgcacgagc acttaatggc actcccgaac cggaagagcg agtgaccgcg aaatcccggt
      601 ttactcacat ctttctttcc tccgggagtt cgctcacctc ttttccgagg aagtaactca
      661 cgacggtcct gatcgtgacg agcgccccga ggatggccag atcctgcagc gttggttcga
      721 ggatcgtctt caagacgtcg gcggcaacga agaactcgag gccgatcagg atacgcgtgg
      781 tgaagaccca ccggatgtcg tggtaagtga tcgctctgat gcgcatctcc cgcgccagga
      841 gctcgatcat cgcgatcgcc gctccgtaga cgatgaagag cgccccgaat gtcccgagga
      901 ctatctcggt gatcccgatg agcgtctcga gcaccatatg cccggcgcgc atatcggcga
      961 gggtgggctt atacgttact gcggttctcc ggggccaggg ggacgacgaa gcggaacccg
     1021 ttgtaccgct cgaccgtcac gggaacgccg tagactgcct cgatcgtctc cggcgtcacc
     1081 acatcggggc tccccgcggc atgaatgacg ccgtccttta agaggatgaa ccggtcggcg
     1141 tagcggagcg ccatgttcag gtcgtgcatc gtcatcaccg cggcaacgtc gtggtccgtc
     1201 gtcacctgcc tgacgatccc gaggatctcg agctggtttt tgaggtcgag gctgcttgtc
     1261 ggttcgtcga gaagcagcac ccggggctcc tgcaccagcg cccgtgcaat gctcactttc
     1321 tggagttccc cgccgctcat ttcgtcgata tagcgtagcg tcaggtcctc aaggtggagc
     1381 atccggactg cagcctcgac gatccggata tccgcatccg tcacgtccca ccggatatgc
     1441 ggccggcggc cgaggaggat cgcgtcgaag acggtcatgc ggctggcttc acatcgctgc
     1501 gggacgtagc ccaccttccg tgcgatctcc atccggtccg ccgtgaggag gttcgtcccc
     1561 tcgacgagga ccgacccgga cttcggccgc aggatggcgt tcatgcattt taagagcgtc
     1621 gtcttcccga cgccgtttgg gcccaggatc gcgaggatct ggtgcggcga gaggtcgaac
     1681 gtgatatccc ggatgaccgg ggcgctccgg taggtgaacg ccacaccgtc gacaccgagg
     1741 atcatcgtcg ataccccgtg aggagaaggt agatgaagac cggtgcgccc aggaacgccg
     1801 tgagcacggc aaccgggagg atatacggcg ccacgatgac ccgggcgacg gtatccgagg
     1861 cgaggaggag gaccccgccc atcacgcagg accccgggat caggtagcgc tggtcgtcgc
     1921 cgatgagtct cctcaccatg tgagggcaga ccaggcccac aaatccgatg accccgagga
     1981 acgagacgat cacggcggac acgagcgctg caacgatcat cccgatatcc cggacccgct
     2041 cgacgctgac accgagtcct ttggcggtct cgtcgccggc gtcgatcgcg ttgtagttcc
     2101 agcggttcgc gacgaagaag agggtggccg ctatcgtgac gagggccatg atcccgagtt
     2161 cctgccagct ggcccggccg acgtcgccga acgtccagaa gacgacggcg gcgagctggg
     2221 tatcgtcggc gaagtactgc agaaacatcg tccccgccgt gaagagggag gagagggcga
     2281 cgccggcgag caccatcacc tcgggagacg ccccgcgtat ccgggagatg agcaggatca
     2341 cggctgtcgc aaggagacag aagatgaacg ccaccatcgt cgtcaggtag gggttgttta
     2401 cggtgacggc gtcggcaacc gtcgaatgca tctctcccgc gccgaggagg atgaccgaga
     2461 cggccgcccc gaaggcgccg gcgttggaga tgccgagcgt aaacggcgac ccgagcgggt
     2521 tacggaggat cgactgcatg gcgacacccg ccaccgagag cccgaccccg gcgacgaccg
     2581 cggcaagcgc ctgggggagg cggatgttcc agacgatcag gtctgtgttg gagatgctca
     2641 cagcagcgcc gggatggaaa agcgcgttga tccggtcgat ggtgccgttc acgagggaga
     2701 gaaagacgtc gtatggggaa atgctgaccg caccaactga aattgatacg atcaggagga
     2761 gaaagaggag gactgccccg ccaagtatcc agagattctt cttccgcacg tatgccaggt
     2821 agtctctcgg gatggccccg tctgcaaagt gcatctcttc ataccctccc atcaggcccg
     2881 gcactacagc gagatctccg cgaacccgag gttcctgtac tggctgttgt gatcttcaaa
     2941 gaccggtttg ccgacgaaga acgtgtagat ctcgtccgct ttctcttccg gatcgacgtc
     3001 ggcgaaccgg tcggg
//
LOCUS       DAJB01000167           13795 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_39192, whole genome shotgun sequence.
ACCESSION   DAJB01000167 DAJB01000000
VERSION     DAJB01000167.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13795)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_39192"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2363 a         4495 c         4580 g         2355 t
ORIGIN      
        1 cgcttattaa acgggagttg agggtactag gtatctccag atctccggca gatttatccg
       61 gacggtatat tctcccggag ggcggttcct cacaactatc atgggttttt tcgcgggagc
      121 cccggctgct ccggatcccg ttcctgaaaa aagtttatcc cttctcgtca gccaggtgta
      181 cgatatcatg gatactgggt ttctccgggt gttgctcaac cccgggcgtt tctttgaagc
      241 gcgcatgcag gacgagccga gcctgaaggt gccggcgctg atcgcgctcg tcatcgggct
      301 gatcggtgcg gtctcggccg cgctggcggc aaacctggtc atcgggatac ttcccgcaga
      361 tatgcaggcc ttcgggatga tcggggtggc gttcgccgta gtcggtggcg tcatcggggg
      421 gttcctgaca tggctcatct tcggtgtcgt cttctacggc atctcgatgg tctttaaggg
      481 cacaggatcg ttcaagcgca cgctggagtt tacgggctac ggatccctgc cccaggtctt
      541 cgggggtatc atcgggacga tcttcaccta cctgctcata tcgggcctga cgatcccgag
      601 agcaacgagc cccgaggaga tcgcggcggt ggcagagaac ctcacgagca tcatggtgac
      661 caatccgctg gcgcagatcg ccgggctcgt gggcatcctc ttcatgatct ggagcgcgaa
      721 catatggatc ttcggcatga aatacgcacg gaacctctcc acgcgggacg cggttctcac
      781 cgtcgggata cccgtgggcc tctatatcct ctacaccctt atcacacttg ccggatggct
      841 gtaacatgaa accaatccac cttgcaatca taacactctt ctgcgccgtc ctggcggtcg
      901 tcggaccggc cagtgccggc ccggcagaca tcaccgtgac ctcctccgcg accgatccgc
      961 cggtcctgat gaagggggat accgggacac tcacggtgac gatccagaac aacggtcccg
     1021 acaccgtcgc catccggagc gccagactct acggcagcgg ggtcgtgccc ctgagcgact
     1081 cctatgcctc ggtcggggag atcggtgccg ggaacagcaa gacgttcacc ttcaccgtcc
     1141 gggcggacgg gggagagggg acattctatc cacagttcgt ccttgacttc cgcgacgacg
     1201 gcagcctccg ctacccggtc ccggtccagg ttgaagatac cccgctcagc gcgtcggtga
     1261 tcggaaagcc ggacgccttc tccgaaggac ggacggccga gatcaccgtc cgggtcggca
     1321 acccccgccc gaacgccgcc tccggtgtcc aggtgatccc gcaggggacg ggcattaccg
     1381 tcaccccgac cggggggttc atcggggctc tcgcccctga cggttctgcg accgtcgtct
     1441 tcaacctgat tccgaccacg gagacggacg tcgccttcca ggtgatctgg cgtaacggca
     1501 tcaacaccca caccaccgac ctcgttctgc caatctcctt tggcgaggac aagcggcagg
     1561 cggacccggt catcacgaac gtcgaggtca cgcccatcgc cggcggctac cggatcgtgg
     1621 gcgacgtcat gaacgcgggc ctcgagtccg cgcggtcggt gcttgtcacc cccggtgcgc
     1681 cggcgacgcc aatcgacccg ttccgggtct acgttgttgg gacccttgat cccgacgaca
     1741 tctcctcgtt tgaggtgaca ttccgggccg acgcaggcac gaccgaggtc ccgatcgccg
     1801 tcgagtaccg tgacgacgac ggcaaccgtt atgcggcgac gacgatggtc ggggtcggcg
     1861 gcgttgctgc ggcacccctc gaaggtcagg acagcggcat accgatagcc ggcatcgtca
     1921 tcgtcgcgct ggtcgcgctc gggatagccg gggcaatcta ctactcctgg agacggacgt
     1981 aaccacaatg ccggtcatca gtttcgagca cgtgagcaag gtctaccccc tccccgccgg
     2041 cgacatcgtg gcgcttgatg acgtcgatat tgccatcgag gcgggcgagt tcgtcctggt
     2101 catggggccg tcagggtcgg gaaagtcgac gctcttaaac atcatggggt cactcgacgt
     2161 cccgaccgca ggggaggtca ccattgcggg aaaccggatc aacgggatgg acgatgatga
     2221 gttgaccctt ctccggcgcg accacatcgg gtttgtcttc cagcagttca acctgatccc
     2281 gcttctctcg gtggtcgaga acgtcgaata cccgctcatc ctgaaaggcc ggcagaacgg
     2341 gtcccgggag cgggcagagg aggtcctcga agcggtggga atcgccaaaa cgcatttcac
     2401 ccacaagccg ggcgagctct ccggcgggca gcagcagcgg gtggcgatcg cccgggcgct
     2461 cgtgaacgac cccgacttcc tcctctgcga cgaaccgaca ggcaacctgg actcaaagac
     2521 cgggaccgcg atcatggacc tcctcgaccg gatgaaccgc gaagagggca agaccgtcgt
     2581 catggtcacc cacgatcccc gaatgacgga atacgcggat cggacgatcc ggctcgccga
     2641 tgggagactc gcatgacctt cttcgatctc gcccgccgga acgtcagccg ccactggctc
     2701 cggtcagtcc ttgcggtcat cgggatcgtc atcggggtga tagccatcgc caccctcggt
     2761 atcctcggca acagcatcgg cctcatgttc ggcgacatgg tcaccgacgt cggcgacacc
     2821 ctcatcgtct ctccggcgac gggtaccacg aaactcaccg aacggcaggt gggcgatatc
     2881 acccgggcgg tcggctccaa tcaggtgata ccgttctcga ccaccgccga taagatgtcc
     2941 gtgggcgata aagagagcgg tgcgatgcta tacgcgatcc ctgccaaaga cattccctcc
     3001 ctgctcgagg tggagtcggg agcctacccg agggacacgg gcgccgggtg cctgatcgga
     3061 agtcagcttg ccgagaacag cagggagaac ggcctgaaac tcggtgcccg ggtcaggatc
     3121 ggcgacgaga ccctccgggt tgtgggcgtg ctcgaagagc gcgggatggg gtttgacatc
     3181 aaccccgact acgctatcgt cgtgccctat tcctggtact cgagccacta cgatgaggac
     3241 gattacgacc aggtgatcgt gaaggtcagg gacgtcaatg atatcgacgc ggtcaaggaa
     3301 tcgatcgagc agaggatgaa ccgccgggag gatgtcgtca acgtcatgga cacccggggt
     3361 atcctcgaga gcgtctttgc ggccaccgag tcgatcacgg tcttcttgac gggcatcggc
     3421 gcgatatcgc tcatcgttgc cggggtctct atcttaaacg tgatgctgat gtcggtgacc
     3481 gagcggatca aggagatcgg tgtcctgcgg agcatcggca cccgccggag cgaggtgatg
     3541 cggatgttca tctacgaggc gctgttcctc ggccttgcag gtgccgccat cggcggagtg
     3601 ctcagtttcg tcgccgggta cctgatgacg gcggtcttcg tcgggaaccc ggattacctc
     3661 ttcgacccga agagcctcct ctatatcgtc tttgggatgg cgttcggcgt catcacgagc
     3721 gtggcctccg gcctctaccc ggcctggaag gcggcgcagt taaacccgat ccaggcgctg
     3781 cgccacgagt gacggcctac tccgtacact tctttttttc ccgctcgatc tcgttttgga
     3841 gcctgccgat gatctcggcg gtcttcgcgg gcgcgccggg gaggagggtg agggactcat
     3901 gctctccttc gacgaccagc agcgcaaccg gcgtcaggca gaccgtggca gcccccccca
     3961 ggtgcacctc aagcgtcctg atgatgggga tgatgcaccg cccaccgacg ttgcgggcgc
     4021 cgccgacgac gagaccggca tcgccgctct ccttctcctc acgcacccgg agcacctctt
     4081 cccgatacct ggcgaagccg ttctctgacc ggtttctgaa gaagggctct tacgaccgga
     4141 acgatgatca ggagtggccg gcggatctgc atccggaacg tgaaggtgcc ttcgaagatc
     4201 ctctggcaaa agaccggggt catcacgaaa tcgatcgcct ccgccggcca gagggcgtag
     4261 cggacggcgg tgcagtagcc gtagatgatg ccggtgtcgg ccgggctatc gaggccgaga
     4321 gtgatatctc cccggagcgt ctcgaggtag agggatcggt agacggcacc gaggatcctc
     4381 tggaggtggg gccagaggtc gccggcggcg tcgaggtatt ctctcgccga aagagtgggt
     4441 ccgggcgcct ctgccttctt ctcctctttt ttgggctctg gtgccgcaat ctcccggaga
     4501 tcccgggtca tgaccgggcg gccgacgagg aggatctcaa gcacctgcag ttcgtcggtg
     4561 atccggaccc tcccccccac gataccccag gctacagtcg ccatcacccg ggcactttcc
     4621 cgtctgcagt cagcgaccac cgagatggtc accggcacca ggtacagggc aagcaggaga
     4681 gagaggagga tgacggcaac ggcgagcagg atgaagaaga gcagggtcgc ggccatagat
     4741 tggaggggaa gagaacctta cgccccttct tcactgctga ccgggatctc ctttccttcc
     4801 tctgagaccc ggggtggttt acccctgcgc tgctgcacca tctcagagcc cttttcgagc
     4861 accttctcga cgtgggggcc gaccgtctcc ccgatcgtcg agatcacctc ggcgagctcg
     4921 ctcttcttct tcagtgacac cacctggata ccctcgggac cggggactcc cctggtgatg
     4981 acgatcaggg caacggccga tatgccgcct ccaccgccgg tcccggaccc gccgtgctgg
     5041 ctgccttccc tgcgtcctcc tgtgccttcc ccgccgccga atgcgaaccc gtactcggcg
     5101 accggtatga taacccgctc gccggcctcg atcggggcac cgaggactgc gctcgcgcag
     5161 agtgttcgtg caagttgatc gacggttatc tggagcatcg attcaccagt cataccattc
     5221 accacaggta ttccagtctg cggcctcgta tataatggtt ggggtccccc ggcggtcgca
     5281 ggatgcttat cccggggcgg atggatacct acttacgctc gtaaggagac caaatggctg
     5341 atggaggatg ctgttgcccc ggactggaac gaggtctgga agaggaggct tgccctgaac
     5401 caatcgactc cggcgttccg ggaaggggcc gatctctggg gcgggcggga gcaggcccgg
     5461 cggtatgccg tccggccgga gaccggccac gcccaccggg tcgcggaggc gatgcgggat
     5521 ctcgcagtct ctcccggcga ccgggtgctg gatatcgggt cgggccccgg gactctcgcc
     5581 ctgccgcttg cccgggcagg tgccctggtg acagcggtcg acccggcgga ggggatgctc
     5641 gccgaactcc gggcagcggc agaagaggag ggactcaccg ccgtcaccac agtccggggc
     5701 ctctgggaag ggatcgaccc gacccgcgac ctgcagccac cctacgaccg ggtggttgcc
     5761 tcattctcgc ttgctatgcc ggatatccgg ttggccctcg ccgcgatgga tgcggctgcc
     5821 tcagggtcgg ttcaccttta ctggttcgcc gacgaaccgg tctgggagcg gctttacctc
     5881 gcgctctggg aagacctgca cggggcgccg taccatcaaa aaccgaaggc cgactgcctc
     5941 ttcaacgtcc tctacggtat gggaatctac gcaaacgtcc tgatgcgccc acttgagggc
     6001 acgaccacgt tcacgaccgt tgaagatgcc gtcgcgcatt tcgcaccccg gatgagcgtg
     6061 gagatcccgg agcaacggga gatccttcgg gactactttg aaaacaatct cgtccggcgg
     6121 gacgacgggc tcgccctcgt cggttcctcg acctacgctg cgatctggtg gcggaaggag
     6181 ggatagagcc cgggccggct ttcagaggag gagacacccg cccacggcca gtgcggcgac
     6241 gatgacgagg ccgaatacaa caaaaatgat ctggcgagtc ccggggcccg caaaccgggc
     6301 gaggagcgcc ccgagttcct gccggtagag gtaggcaaac agtccggcga tcaggaagag
     6361 gaagagccac tcactcggct gggcgagttc ccgcaggatg gcgtcccaga agaagtcttc
     6421 tgcgtagttg taccggggta tcggtccgag gaacggccag aaccactcca cagggtgccg
     6481 ccacatcgca tcgaagaact ggtggcttgc catgcccacg cccagtgcaa ggagaccgat
     6541 cttcccgcgg tagcggtaga ggaggatgcc ggcgaggagg atcagggtga ggatggtgag
     6601 gccgtggaag tagatccgac cgtagttgac cgtcccgttg aggatgatat gcccgagcgg
     6661 cttgtcgatc aggtcgggga gcacggcgcc cagcgcggcg agcgtgagaa cccgccggtc
     6721 gccggcgatc gccgcgatga cgagccctat gaggacaccg accatggcat gggcgatgag
     6781 gtacatcgct aaccagtggc ggggcgatca tataccagtt ttccccggcg accccggttc
     6841 ctcccgaccg gggagcgggc aggaactccg cgatcctgcc gtaccaggat atccttcact
     6901 gatcaataaa gatacgtaat gtatatatgt gtactgagta caccatgatg tatgcagacg
     6961 aagatcctcc ttgacgagga ggagatgccg aaacggtggt ataacatcca ggcagatctc
     7021 ccaacgccga tggatccggt cctccacccg gggaccggga aacccgcagt ccccgacgac
     7081 ctccaccaca tcttcccgat ggaactgatc cgacaggaga tgaacacgga ccgctacata
     7141 gatatccccc aggaagtccg cgaaattctc accctctgga ggccgagccc tctctaccgg
     7201 gcaagaaggc ttgaggcggc cctaaagacc ccggcaaaga tctactacaa gtgggagggc
     7261 gtgagcccgc cgggctcgca taagcccaac accgccatcc cccaggccta ctacaaccgc
     7321 gaagaggggg tggagcggct cgcgaccgag accggggcgg gacagtgggg ctcggcactt
     7381 gcgtttgcaa cgggcctctt tggtatggag tgcaccgtct acatggtccg gtcctcctac
     7441 gaccagaagc cctaccgcaa aagcatgatg caggtctacg gcgccgagtg catcccgagc
     7501 ccgtcgccca ggacccggtc gggccaggcg atactggatc gcgaccccga gactcccggt
     7561 tccctcggca tcgccatatc tgaggcggtc gaggacgcgg ccagccacga aaacaccaac
     7621 tacgcgctcg gctccgtcct caaccacgtc tgcctccacc agacgatcat cggccaggag
     7681 gcacagcagc agctcgcgat ggaggacgtc taccccgacg tcgtgatcgg gtgcgtcggc
     7741 ggcggtacca actttgccgg gctctccttc ccgttcgccg gcgataagat gaccggaaag
     7801 caccccgaaa ccgatatcgt cgcggtggaa ccttctgcct gcccgaccct gacgaagggg
     7861 ctttacgcct atgacttcgg ggacgtcgcg gggctgactc cgatcatgag gatgttcacc
     7921 cttggccacg acttcgtccc gccggcgatc cacgccggag gtctccgcta ccacggagcg
     7981 tcgccgctcg tctcccggct tgtccacgat ggggtgatcc gggccgtcgc ctaccaccag
     8041 aacgaggtct tcgaggccgc cgtgaccttc gcccggacag aggggatcat cgtcgcgccc
     8101 gaggccgctc acgcggtgaa ggccgcgatc gacgaggccc tccggtgccg ggagaccaaa
     8161 gaagcaaagg tgatcctctt caacaactcc gggcacggca actttgattt ctcctcctac
     8221 gaagcctatt tcgcggggaa acttgtcgac tacgaatacc cggcggagct gatcaaagag
     8281 tcgctctccc ggctaccggt gacggggtga tgccggtgga ctggacaggg ctcgaagaga
     8341 gctgaacact gagccggggt gtgaggaggt cgtcgccgcg gcggcgcgtc gcgagcggcc
     8401 cctcatcgtc ccggtattta ccgatatccc gctccccgcg tcttcaccgg ccgacctcta
     8461 cgcctccctt cgggacggtc cggggttcct gctggagtcg cttgagggga acgagaaggc
     8521 cgcctgctac tcgttcatct gcaccgcccc ggccgcgacc atcgccgtga ctgccgatgg
     8581 cgcggtgaca gtctccggcg acccccgcat ccgtgagatc gctgccggta tcgaggccgc
     8641 cgacgccgtc gacgccgtcc gcttgttcat gggccggttc cgggttgccc cctcgccgct
     8701 ccagaggttc tcgggagggc ttgccggcta cttctcctac gacctcatct cttctatcca
     8761 cccgatgttc cgggtggaac cggcggagga tcttaaagag cccgtcgccc ggttcatgct
     8821 cgcgcgggac tgcctcgctc tcgaccacca cggagagcgg ctcgcagtca tcgggaacct
     8881 gctcatcacc gacgggacgg atgcggagga ggagtatcac cggggccggg cagcaatcgc
     8941 cgaacgggtc gcccggatac atgatctcat tccggcaagc ccgggcgatc cgggcaggtc
     9001 cggcgttccg gcatcgtcat gcacccctga ggagttttcg ggagcggttc tccgggtcaa
     9061 ggagcatatc gcggctggcg acatcttcca ggccgtcctc tcccggaggc tcacctgccg
     9121 tatcgagggc gatccgttcg atgtctaccg gcggctgcgg gcaacaaacc cgagccccta
     9181 catgtactac ctggactttt cggactgcca ggtcgccggg agcagccccg agatgctcgt
     9241 ccgcgtggag aacggtcggg tgacgacggt cccgatcgcc ggcacccggc cccgggggtc
     9301 gactccggcg gaagacgaca ggctcgcggc cgagctcctc gccgacgaga aggagcgggc
     9361 cgagcatatc atgctcgtcg accttgcgag gaacgatgtc ggcgcagtct cggcctatgg
     9421 gagcgtctcg gtgacggatt tcatgaccgt cgagcggttc tcgcacgtcc agcatatcgt
     9481 ctccacggtc tcgggcacgc tccgcgaggg atgcgaccgt ttcgatgcgc tgcggtcctg
     9541 tttccccgcg gggaccgtct cgggggcccc gaagatccgg gcgatgcaga tcatcggcga
     9601 gatggagggg ctccggcgcg ggatctacgc cggggccgtc ggttacctcg gctttgccgg
     9661 cacgatggac cttgcgatag ccatccggac ggttatcgtg gagaacgggg tcgcctccgt
     9721 ccaggtgggg gccgggatcg tggccgactc cgaccccgcc cgggagtggg cagagaccac
     9781 cagcaaagga cgggcgatgc ttgcggcgct cggtgcggag gtggaatgat gcgggtgctc
     9841 gtcattgaca gttacgacag tttcaccttc aacctctgcc agcagatcgg gatgctcggg
     9901 gcggaaccgg tcgtggtgaa gagcgacacg ccgtttgagc ggatttgttc cgcatccttc
     9961 gaccgggtcg tcctctcgcc cggcccgggg catccccggg actccgccct ctaccaggcg
    10021 gtccttacgt caatcagcag gaccgtcccg acgctcgggg tctgcctcgg ccaccaggcg
    10081 atcggcctca ccttcggggc ctccatcgcc cgggccgacc ggctgatgca cgggaagcgg
    10141 tcgctcgtcc ggcacgacgg agcggggatc tacgcggggg tgcccgatcc cctcgtcgcg
    10201 acccgctacc actcgctcgt catcgaccct gcaaccatcc cggactgtct cgaagtgacg
    10261 gcccgaagcg acgacgacgg ggcggtcatg ggtgttcggc atcgggagtt cccgatcgag
    10321 ggggttcagt tccacccgga gagcatcctc acccccgacg ggaaccggct gatagcaaac
    10381 ttcctcttcg gcacgggggg tggggcatga tccgcgaggc gattgcccgg gtatcctcag
    10441 gcacggacct caccccggcc gaagcggggg aggcgatgga agagatcatg cggggggcgg
    10501 cgacgcccgc ccagatcggc gggttcctca cggcactgcg gatgaaaggg gagaccgagg
    10561 tagagatcgc ggcgttcgcc cgggtgatgc gggccgccgc cgtcccggtc ttcctcccgg
    10621 gtcctgaggc gcgggtggac acctgcggga ccgggggcga cggtgtgggg accttcaata
    10681 tcagcaccgc ggccgccttc gtcgcggccg gtgcgggcgt ctccgtcgtg aaacacggga
    10741 accggggggt gtcgagccgg tgcggctcgg ccgacgtcct cgaggcgctc ggggtcgacc
    10801 tctcgctgtc gcctgagcgc gtggtcgagg tggctgagca ggcgggcatc acgttctgtt
    10861 tcgcgcagac cttccaccct tccttccgac acaccgccgt tccgcgtcgc gagctgggca
    10921 tcggcagtgc gctgaacttc ctcggtcccc tgaccaaccc tgcccagccg accttcgcgg
    10981 ccatcggctg cgcggacgca ggcatggctc cgctgctggc cggggtcttc gcgagtcgcg
    11041 gcaaggatgc tgcggtcttc cgcggcgatg acggactgga cgaggtcacc gtggcgacca
    11101 cgaccagggt gtggtgggtt cgcaatggcc agatcgggga ccgcagcatc gatcccaccc
    11161 ggctgggcct cgagctgcac cccacggagg cgctgcgggg cggcgacgcg atgcacaacg
    11221 cacaggtcgt gcgcgaggtc ttcgcaggtg cgaccggggc ggtccgtgat gccgtcctac
    11281 tcaacgccgg gatcgcgctg gccttgaccg agcccgactc gggcgcgact caggccgatt
    11341 tcgaccgcga cctgcgcacc gccatggacc gtgcggctgt ggccatcgac tccggagcgg
    11401 cgtcgcgcgt catcgaccgt tgggtcgagg cgacccacgc ctgagcagtc aggcgccggt
    11461 cactccccgt cgagtccgat gctgaaggcc gcctcgaggt ccttcgagga gtaggtccgg
    11521 aaggcgatgt gcgtcgatgt gccgacgacg cccttgatct tgttgagtcg gtccgcgatg
    11581 aggtctgcga agtcctcatg cgccttgact cgaacgagcg cgatgaggtc ggcgtcgccg
    11641 gtcacggagt agacctcggc gacaccgggg atctcggcga tctcctgggc agcctcgggg
    11701 atgcggttga cctcgcagtt gatcatgacg atggcggtga tcacggccca accctatccc
    11761 gcgcgtcgga agcgtctcga tgccgctggg ccagacctct cggtcgtggc gtcccgatcg
    11821 aatccgacca cctcgcgagc cgccgccacc gagggctcca gctcgtgccg cagcgcaccc
    11881 gcaccgccga ccgggcaggt ccaggacccg tccagctcga cgaggcgcac accgggggcc
    11941 tccagccagc tcaagaggag ctccgtctcg gcagtcagcg tggccgccgt gagggcctcg
    12001 ggcgcactga cgacctcggc ggtggcctgc atggtccgca cgtgcaccat ggggtcagcg
    12061 ccgcgcgggg tgaccaccgt cgcggcgagg cgtccgtaac ggatgcacac gagctcccag
    12121 ccaccgtcct cgtgccgtct ggccgccacg atctccggcg cacgggccac gggccccagg
    12181 cgttgggcgc gcgccgcgcc gcggacgagc tggaggaacc tgtcgcgaac ccgggccgcg
    12241 tcctcgaacc gctcctgcgc ggccagctcg tcgagccggg cccgcagtcg ctggccgccg
    12301 ggacgggcgt tgccactcag gaccgcggcg cagtcctcga cgacgagcgc gtagccggcg
    12361 tccgactggg cgccggtgca cggtgcgccg cagcggccga gatcggccag ggcgcaggcg
    12421 gactgccgtc ccgtgatgcg ctgggtgcac tggcgcaagg ggacgatgtc gtgcacggcg
    12481 tcgatggcag cttgggcagt gacgcgggag cggaagggcc cgatgtagtg ggcgtcgtca
    12541 tcgcgcacct ggcgcacgat ggacaggcgc gggaaccgct cgcgggtcag cttgacccac
    12601 atcacgcgcc ggccgtcggt ggacctgcgg ttgaagcggg gcttgtgctc gtcgatgaga
    12661 cgcagctcgc gcacctgtgc ctcgagcggg gtctggcaga cgaccggcgt cacggcgtgg
    12721 gcgaggcgaa ccatgtcggc catccggctg cgctgctcgg aggccgtgaa gtaggtccgg
    12781 acgcgggtcc ggatgtcacg gctggtgccg acgtagagga cgcggccgtc gccgtccttg
    12841 aagaggtaga cgcccggcgc gtgggggagc ggatcggcca ggaatcgctt gcggcgttgg
    12901 gcgggagtca cgcgggaggt gtagctcgcg agctcctcga tggtgtgcac accgaggttg
    12961 cccacccgct cgatgaggcc gtggagcacg tccaccgtgg cccgggcgtc gtgcagggcc
    13021 cggtgatccg gggtggtggt ggccccgaag acccgagcga gcgaggccag ccggtggttc
    13081 ggcgcctcgt cgcgcgtcac gagctggcgc gcgagcgtga gcgtgtcgag cacgtcgaat
    13141 ccgggccacg gcgtctgggt gcggcgagcc gccgacttga ggaagctgat gtcgaagccg
    13201 gcgttgtggg ccaccaggac gctgccggcg gcgaactcga ggaaggccgg aaaggccgac
    13261 tcgatgcggg gcgcatcggc caccatcgcg tcggtgatcc nnaccagggt ctggaactca
    13321 ccgagcacct cgccggcacg caccttgacc gccccgatct cggtgatgcc gcactgctcg
    13381 gggctgccgc cggtcgactc gaggtccacg acgacgaacg tgacgtcggc caaccgggtg
    13441 ccgaggtcgt cgaacgtcgc ctggatcgcg tgcatgaagc gaccgtatgc cgctgggctg
    13501 acactgctcg gaaggcgcgg cgggatgccc gggcagttca ccggcagccg ccgtcggtcc
    13561 gctgtcagtg ctctcccata gcgttcttct cgacagttcg caatgacgaa gaaagggcgg
    13621 agccgatgat gctggtcgac tgccacacct gccccgtcag ggacgtccac tgcgctgact
    13681 gcatggtgac ggcgctggcg gccattccga tgcgtgcccc cgaggtcgac gagagcctgc
    13741 cgctcgagcg cgccgagggc ctgccactcg accgcagcga gcggcgagcg gtgtc
//
LOCUS       DAJB01000168            4571 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_123794, whole genome shotgun sequence.
ACCESSION   DAJB01000168 DAJB01000000
VERSION     DAJB01000168.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_123794"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          964 a         1281 c         1337 g          989 t
ORIGIN      
        1 cgaggtcgtc gaggataagc cggtcaagag cgctctcaag atcggtgcac ccgtacttct
       61 caaggaacat atagtagtag atcgtcccgc cgaagaggag atagagatcg agccggtccg
      121 ccgggtcctg cacgccaagg tcactgagca tggcaagaca ctcttccgga ccaaaggggc
      181 gcagcgtcag gatgttgtcg gcccgcttga agagaggtgc tcctccttcg aggaagatct
      241 cccggatcat cccgaccgac gagcctgaga cgatgatgaa gaggtgagaa ttccgtccct
      301 tcaggtccca gaaacgctgc atctgtgaga tgaacgaggg gtggatcttc tggaaccgct
      361 gaaactcatc aaagacgacg atcagcggac gatcgtacga gaagaggaat tccaggaatg
      421 tctccggggt atcggtccgg atatagccgg gaagatcgag cgtatcgatc atcagccgcc
      481 cgaactcctc catcaggtct tcgatgctct tgttcgcatc gacgtaaaag tagagggcct
      541 gcttatcttt acagaactct aaaatcagcc cggtctttcc gacccgcctc ctgccggtga
      601 cgaccaggaa ggagggggct ccggcataga ggtgctccat cagtgcaagc tcgcgcttcc
      661 ttccgtaaaa tctcataatg attattaata ttattattat taatataatc actatcacgg
      721 gggtgcctgt cactctcccc cgggagttac cgcagtgaag agggtggagc gggaagatcc
      781 cggacgcctg tatcttatag ttatatttac ttatacaact aaaagtcact catgccagcg
      841 tgtaaccagg gatcacacag tccccagctc gacacgatcc ggatggtcga agaattcatt
      901 caggagcaca gcggcgaata caaacggcgg gccttgtggg agaacctgcc gaagaaaatg
      961 atgtaccaga cgttcaagac gatcatcgag tatcttttgg agtcggggaa gatagcaagc
     1021 gattcgcggg ggacaatctg ctggattcac gacccggata tggtcctgaa gtatgcaatg
     1081 cgcgaagacc tgcggttccg atgaagtgcc gaatctattt tgcagacgag caggtaaggg
     1141 aggccttcct ggctcttcag gcctcccagg atcccgggga tcgtcggcta gctgaactgc
     1201 tggtccgggc tctcgacgga atcgcggcag atgccttctg cgggatccag gtgccgaaga
     1261 agctgatccc gaaagaatac cacaagaaat acgggccgct gaagaatctc tggaagtata
     1321 atctcacccg atcgtggcgg ctcgtgtata ccgtagtccg cgacggcgat ataaacgcgg
     1381 tcatcatcga atggctggac cacatcaact atgatcgccg gttcgggtac tgagtgttac
     1441 ctcccggcag atttgccggg atgtgccgat cgccggaggg cgggccatgt cacccgacgg
     1501 ggtgacgtca ggaccccttc agggtgacat ccctcgcggt atgggggtcc ggctcccggt
     1561 cagggccctc tcccgggccg atgttgcagc gctgcccctt catcaggcat gtttttcgtg
     1621 acggcgtggc ggtttagtgc cgtagttatc ccggaggacc ggaagctcac gctcaaagaa
     1681 gggccaagac tcgtggcttg acggattggg attctttcac catcgaacgt caccgggatt
     1741 taccgatggt cattctttgc catcagggga tatgaggttt tgccggtggg ggttggcctt
     1801 cagatcgcac cggttgatca cgcaatctcg ctctaccttg aagtcgacga gtcgcctgaa
     1861 ccgggcgaat tacggaagtt gtgggttcag gctgttggag tgaggtcctg tcggggctga
     1921 aaaggtcatg aactatcagc acagcgacgc cccctacccc ggctccggga tcgttccgtt
     1981 cggtcatcct cagtcttcag cgagcagggc ccggtagccg gcctggacaa catgccggtc
     2041 gccggtcagg gcatcggaga gcccctgctc cttcatgacc gcaagggaga tacaatcagt
     2101 aagactccag gctttatcca gccgggtctc gtagatgctc catcctttcg accataggtc
     2161 gggatcgaca aagacaatct ccacccgagg tgacgattca aggcggcggc gaaatgcgat
     2221 gacggacggt ttgaatgcag gatggcagag agcgttggac gtctcgatga gcacggagga
     2281 cgtggtgacg aaaccggatc ctgactccat tcggtctttt tagatagttt cagcggtttt
     2341 gtggaggttg tcgtgcctgt tcagcagcgc gatccagcaa gccgaatcga caaaacacct
     2401 gtcagtcatt catattgagc ggtagacaac atgcacatgc ggacaataga actatccgat
     2461 gtggctcacc atcccgcgag gtaagtattt agttcgacag caccacatga tctcactggc
     2521 atgaaagtca attgccttat cgaaaaagac gaggatggtt attactacgc ctcggttccc
     2581 tctctaccgg gatgctttac acaggcaaag acttacgaag aattgatcgt acggcttgat
     2641 gaggcgatat ccctctacct tgaagtcaac gaattgcctg aaccggatga gtcgcgagag
     2701 tttgtggggg ttcaggcagt tgaaattagg tcctgtgaag gctgaaaaag caataaaagt
     2761 ccttgaagaa tccggttttt tgtttgcccg acagaggggc agccatgtca ttctgcagca
     2821 ctccgatgga aggagtatcg ttgtccccgt ccatcaggga gaagaactcg ggagaggggt
     2881 gttacgggcg atcatccggc aggcaggcct cacccgggag gaatttatgg tgaggctact
     2941 gaaatatcgg tgaagataac gtttattgca gatcgacgag gatgcgcagc gtctccacgt
     3001 tctcccggct ctgcgtcgag aagtttgcga gatcggtctc tgagaggtcc agggagacga
     3061 ccaggctgct catcgaccgc gtcatgttat tgtgcgaggt tccgctgcgc gctctcccgg
     3121 tccttcacct tcacgtctgc gctcctcgac gaagtacccg actactgcga cgagaccttc
     3181 tgttccgtcc gggaagaccg ggaggaggcc gtcggcgatg ccggggaggt gcgcctgctc
     3241 gcccgtttca tcgaggagaa ggggctctga gtcttgcagg accggcgcga tggaggctac
     3301 ggcttacccg gtgccttccg ggagcgtgac ttcacccacg ccagcgcacc ctccgttcct
     3361 gcccatcccc tgcctgcggt agtgacatag atgatcgcct cgttccggtc gccgaggccg
     3421 tagtagtcgg gcatccgggg cggcaacttc cgccgtccga gcaggtactg cgggacgaac
     3481 gggtttttct gcaacgcgtc gtcgagtgcc gcgtcggctg caggtccggc cccctccttc
     3541 cgaaacagcc agagcgcttt tgagtatgcg accgccgccg tgggatcttc tccatactcg
     3601 ttcaggagcg cctcaaacag gtcgtcccgg ttcgcctccg caaagtactg gatgatgatg
     3661 taccggaccc cctggttgtc atcgggattg agccgcagca tctcctgata cagcccgatc
     3721 gcttcgtccc tgtccccggt cacccagagg aggtctgcga gtgcaagcag ggccctcatg
     3781 tagggacgcg tctcaatgat cgaccagaag ttcccgacct cctcttcaaa gacctccttc
     3841 ccgagtgccc gctccccggc agccacaccc ttccgggcgt actccagcgc tcttcgcagg
     3901 ctttttgtct cttctccggc cagcagcacc catgcatcgg cgcagtcctc ggagatctca
     3961 agcgcctgtc tggccagcgt cacccggcgg cgtccggtgg cttccattgc gtcgtacgcg
     4021 acctgctgcg cccgcttgag cggggtatcc gcgggagcgg gcggcggccc tccctctttc
     4081 agcacggttt gaaggaaggc gttcgcctct tcgagcgatt caaattccct gtccttcatc
     4141 aatgccgtca cgtcgatcaa cgacctctcg tttgccgccc gcattgccat atggttcagt
     4201 cgctttgctg ccatcttcaa cgtagcagtt agtgtgctct accctgatag agtttctgtc
     4261 ccgggcgctc acgaagccgg gatggatggt ggccccttcc agggggtact tctcgatggt
     4321 gccggggagg gcctcctgat cttgaggtga agagggcgga gcgggaagac cccggacgcc
     4381 tgtaggttat agttatagtt acttatacaa ctgaaagtca ctcatgccag cgtgtaacca
     4441 gggatcacac agtccccagc tcaacacgat cctgatggtt gaagagttca ttcaggatcg
     4501 tgggccgagg agcccctcga cttaagtcgg gggaggaatc ggccctcgcc gtgtcccgat
     4561 acacgttcac c
//
LOCUS       DAJB01000169            2690 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_97557, whole genome shotgun sequence.
ACCESSION   DAJB01000169 DAJB01000000
VERSION     DAJB01000169.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_97557"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          489 a          822 c          884 g          495 t
ORIGIN      
        1 gcaatcgaga gggcggcgac ggtgatggtg atcgcctgcc cgcacgccct tggcctcgcc
       61 gtgcctctgg tggtcgcggt atctaccgcc ctcgcggcgc agtccggtct cctgatccgg
      121 gagcggcagg cgtttgagcg ggcgaaggac gtgcaggcgg tggtcttcga taagaccggc
      181 accctcacgg agggccggtt cggagtgacc gatctgctgc ccctgggagc gatcgccgat
      241 ccgctcgagg tgttgcgtct taccgcgtcc ctggaggcca actccgagca cccgatcgcc
      301 cgcgggatcg tgcggagtgc agaggagaag ggccttaatc tcgttccggt atcggggttc
      361 cgcgccattc cggggaaagg gatcgaaggg tatgccgacg atgcgttcct gaaggtggtc
      421 agcccgggct acctgaaaga agcgggaacc gaaccagccg atgagcgggt cgagcagctg
      481 aagcagcagg gaaagaccgt ggtcttcctg ctccgggacg atacgcctat cggggtggtc
      541 gcgctcgcgg atatcatccg gggtgagtcc cgggaagcca ttgcacggtt aaaagagatg
      601 gggatccggc cgatgatgct gaccggggac aaccgctacg tcgcgaagtg ggtggctgac
      661 gatctcgggc tcgatgctta cttcgccgaa gtgctgccgc acgagaaggc ggagaagatc
      721 aaggaggtgc aggctcagta ccgggttgcc atggtcgggg acggggtcaa cgatgccccg
      781 gcgctcgttc aggcggatgt ggggatcgcg atcggggcgg ggaccgacgt ggccgtggag
      841 agcgcggata ttgtgctggt ccagaacgac ccccgcgatg tggcggatat aatactcctc
      901 tcgaggaaga cctacagaaa gatggtgcag aacctgctct gggcgacagg ctacaactcg
      961 ttcgccattc ccctggcggc agggattggg tatggggtcg gcctggtgct caccccggcg
     1021 gtcggcgcta tcttcatgag cgccagtacg gtcatcgtcg ccatcaatgc gaggacgctc
     1081 tccctgcact gatatcgaac gcggcagccc accccctcct tcatacccct cgttcccgac
     1141 gccggaccgg aattacattc gggcgccgcg gccgagcact tcggggttca ccacattctc
     1201 cgggtgaccg gccgcgaact gctcgatgtt tcgtatggtg gtgaaggtgc attcgtccat
     1261 cgactcctcg gtcaggaacg cgatatgcgg cgtcaggagt acgttatcga gctgccggag
     1321 cggactctcc gcagggagcg gctcgtgctc gaagacatcg aggccggctc cggcgagacg
     1381 tcctgtccgc aacgcctcga cgagcgcccc ctcatcgatc acccggccac gagcggtgtt
     1441 gatcaggatg gcgccgggct tcatcagcgc cagttcccgc gccccgatca tccgctcggt
     1501 cgacgccgtc agggggacgt gaagcgtgag gatgtcggat tcggcgagca gcgtcggcag
     1561 gtcgacgaac tcgaggccga gccgccgcgc ccgttcggga ccggggtgcg ccgtggtcga
     1621 gaggacccgc atgccgaacc cgtgggcgat ctggatgacg cgtgtgccga tgtcgcccgt
     1681 gccgatgacg ccgatggtca tacccatcag ctggtgacca cagtattccc gccagtcaaa
     1741 tcggccgccc cgaagccggg catcggcggc atggacccgc cgcagcaggg cgaggacgaa
     1801 ggcgaagacg aactcggcca ccgcgtcgaa cgcgtaggca ggaacgttcg agacaacgac
     1861 ccctgcgcgg gttgccgctt cgaggtccac atggtcgtaa ccggtctgcc agaccgcaac
     1921 catctgcagg tgcgccgccg cgtcgaaggc gtcggcatgc atcccgtatc ttccgacgac
     1981 cacgacctct gcatcacgga tacggttccg gaactcggcg tcgaagatcg ggtacgtgtc
     2041 gaatatctcg acctcgccga ggaggtcgag ttggtgacgg taggcctccg tcaggaagat
     2101 cgggtcggtg atgacgatct tcatgtctgc atcgcccgca ggtacatctc ggggttctcg
     2161 tcgaacttct tcttgcaggc gaggctgcag aacgagtagg tcctgccctg atactcgctt
     2221 gtaaacctga cctcccgctc ctcaagttcc atgccgcaga ccgggtcttt caccgtcacg
     2281 atccgtcacc ccctggttct ccattcaccc cgggagtggg tatctccgcc cggcagccac
     2341 gagggccccg gtaagcgggt gcgtcccgga gatcccgaga accgcgacgg tggcgtctgc
     2401 gactcgcttg cccactgtcc cgtatctcga ctgcgacccg gatcatagga tgtgcccatc
     2461 agcgatattt tatatatacc ttttcccgga tttcagaaaa ggccggattt agtgtattat
     2521 tttggtattc cgactacacg gactgtttta gaacacggga tacatctcgc cgcaagggac
     2581 acggcggcct gatgagatgc ttgttcccat cagggtgata ctgacataac cgcgaagagt
     2641 gatgaggctt gcagagagtc aactaccccc ccctaacgga gggggcttgc
//
LOCUS       DAJB01000170            2600 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_123793, whole genome shotgun sequence.
ACCESSION   DAJB01000170 DAJB01000000
VERSION     DAJB01000170.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_123793"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          520 a          725 c          881 g          474 t
ORIGIN      
        1 cgaggatcca gaacgtcttc tggggaaggc gcagatagtt gctgacgact cgcagtgcca
       61 ttatctctta tctttggtgc tcatctgtct ataagagaac tcccgcgggg cgaaagtata
      121 cggggcttga tgcgggcaat tcctcccccc gcttgcgcag ggggcctcct tgcccgtcgt
      181 atgtgaaggg cgtctgcatg acgccggtag tgcaattggt gtatcaatgg gcaaacgtgt
      241 accggtaaca tctgggaaag agcaaaacgt ccctatgccg gagtcccctc ctctctcggg
      301 ggcgcctgga agagggcgga cgcccgtgcc acaggctcgg gcatgcccag gacgatgacg
      361 atatcgtcgg gcaggatctc cgtctccccg cccggattcg tgatcgtctg ttctccccgc
      421 aggacggcga ggaccagcac gtcgtgcctt ttgcggaggt tgatctcacc gagcgtcttt
      481 cctgccaggg gggaggcagg atcgacggtg taggtgacga tgctcacgtt ggagagccgg
      541 tagaccagat ccgtgagcgt cggagggtgt tctttcgggt tgcggagcat ctggtaggtg
      601 tccgagcgaa tctcggcggt cagcccctcg atccggtcct tcgggatgag gtatttgttc
      661 agcacccggg tgaagatctc aagcgacgtc tcgaactcct ccgggaccac ctcgttggcc
      721 ccgagatcct gcaggggcgc cacctcgatg aggtagcggg ttcgcacgat gatgtagagg
      781 gacgggttta agcggcggca ggcctccacg atctgtcggg tcgagacggg atcgttgatc
      841 gcgatcacgg caatacgggc ggactcgata tcggcgtgca ccagaaccgt ctccgtggtg
      901 gcgtcaccgt agtggatggg ttcgccgaga gcccgctctt ttcgcacggt ctcggggttg
      961 atctcaatga tgacatacgg gatcccgccg actttcgccg ctcgcgcaag gttcctcccc
     1021 gtcacgccgt agccgatgat catcacgtgt tccctgaggt gctcccgctg gggctcctgt
     1081 atggggcggc gggcgtccag ggtccggtgg agaaacccga cgcggcggag gcgatcggag
     1141 agcatcggtc cgccgccgat gacgaacggt gtggcggcca tcgtgatgag ggcggtcacc
     1201 aggaagagct ggtagctctc aggggagagg atgccgtaat caagaccgct tctcgagagg
     1261 atgaaggaga actctcctac ctgggcaagg gcgagcccga cgagggtgat ggtgcggatg
     1321 gggtagccca gaaccaccgg aaccacggcg gcgatcagtc ccttcgcggc gatcacgccc
     1381 acgatcagga gaagcaccag tccgaggtgc tcgagtaaga accccacgtc gagcagcatc
     1441 cccaccgaga caaagaagaa actcgtgaag atgtcgcgga acggcacgat gctgccgatg
     1501 gcctgatggc tgtactccga ccgggagatc aggagtccgg cgaggagggc gcccagcgca
     1561 agcgagatcc cggcgagcga gacgagccag gcgacgccga agcacgtcac catgatgaag
     1621 aggaggaaga gttcgcggtt cctcgttctc gctatctggt aaaggaacca gggaactccc
     1681 cactttgcgg ccacgacgag gatgaccaga atcaggatgt cgatggtgag catcgacgag
     1741 agagactcca tgctgaacgc ctgcactgcc gcgggtgcgg agatgcttgc gaggaacggt
     1801 atggccatga tcatcgggat ggcgatgatg tcctggaaga tcaggatccc gaggatgatg
     1861 ttgccgtgcg gggtgctcat ctccccccgc tggtggagga tccggagcac gaccgcggtg
     1921 ctggagaggg cgaagaggaa acccagaagg acggcttctg caaacggcag cccgatgagc
     1981 gtcgcgatga agaaggagac caggaacgtc aggaagacct ggatcgatcc gctgacgagg
     2041 agcatcttcc gtatctgcca gagatgggcg aaggagaact cgagaccgat ggtgaagagc
     2101 agcaggacga tcccgatctc ggcaaggatg ttgacctctt cgaccgactg cacgatgccg
     2161 agcccgttcg gcccgacgat gatgcccgtc aggatgaacc cgaccagcgg cgggatgcgc
     2221 aacgggttaa agagcagccc gacgatgagg gagattccga agatgaggac gatggcgttc
     2281 aagagttgca gttccatttc cggctcccgt gcttcgttct atctgttttc ggttttgagc
     2341 ctcatgtagg tatcgggctg tccgggtgcc gggccatccg gaggaagggc ggcgttccgg
     2401 ctcaggctcc agaaagccgg ccgcgacacc tgagagatcc ctgaaaaacg gcttcttcgt
     2461 gcaataaccg caggatatct gaatgcgggg cggtctcgtc gcccggtctc gggtccatgg
     2521 ggcccacctc ctccctcctc ttctggaggg gggcagtcga tgcgataggc ccgttattca
     2581 ggatcgtggg ccgaggagcc
//
LOCUS       DAJB01000171            4806 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_109396, whole genome shotgun sequence.
ACCESSION   DAJB01000171 DAJB01000000
VERSION     DAJB01000171.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_109396"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          837 a         1561 c         1537 g          869 t
ORIGIN      
        1 ccatctgggc aatctcgagg gtctcaagat ccgtgatccg caggccgccg acgaacctgg
       61 gctccttgcc catcgcctgc atctttgtgg tgatctcggg gcctccgccg tggaccagga
      121 cgaccttcat ccccacgtag cggaggagga cggcgtcccg gatcaccgta tcgaggatgt
      181 tctggtcgac catcgcgtgg ccgccgagct tgatcacgat cgtcttgccg tgaaacttct
      241 ggatataggg gagtgcctcc atcagcacgt cttctcgctt catgtcgtat acttcccgtt
      301 gatctctacg tacttctccg tgaggtcgca tccccacgcc gtcgccttcc cctcgcctgc
      361 ggcaaggtcg aggtcgaacg tgacggtgtc gccgcgcatc gcggccttcg ctcttgcaag
      421 gtccgcaacg atctcgccgc ggaggacaag cggcgtccgt ccggcaccct cgccgacaga
      481 gagcgagacc ccataggggt cgaactcaac gccggcccta ccggcggccg cgaccacccg
      541 gccccagttc gggtcttcgc cgtagacggc ggtcttgacg agcggagagg cgacgatcgt
      601 ccgtgccacg gcggcggcag cgtcctcgtc gggggcgccg cggaccgtga ccgcgaggag
      661 tttcgtcgcc ccctcgccgt cgcgggcgat ctggacggcg aggtcgcggc agacggcctc
      721 gacggccttc gcgagttcgt cccggtcaac ccggcctgcg gcccccgtcg cagtgcagag
      781 ggccgcatcg ttcgtgcttg tatccccatc gaccacgacc cggttgaagg accgccgggc
      841 tgcctggcgc aggatatcgt gcagaaccgg cgcctcgacc tctgcgtcgg tgtagaggaa
      901 cccgagcatc gtccccatgt tcggggcgat catcccgctc cccttggtga tcccgccgac
      961 ggcaaacgac tccgtctcga cgagggcgtg tttggggaag gtgtcggtgg tcatgatcgc
     1021 cttcgccgcc gcgtcctcgg catcgccgtt ccgggcaagg agcggcgcga cccgcccgca
     1081 ctggtcgcgg atcaggggga gattcaggta gcgcccgatc accccggtgc tcgcgacacc
     1141 gaccgactcc ggggcgaggt tgagggattc gcccgcaatg gcgcacatct ccaccgcgtc
     1201 gcggtatccc cgctcgccgg tgtaggcgtt cgcgcacccg ctgttcacga cgatggcgtc
     1261 gagggtgccc cggcgcatcc ggtccatcat cacctcgacc ggggccgccc gcaccctgtt
     1321 ggaggtgaag acgccggccg cggtcccgct cgcccggatc agggcaagcc cgaacttccc
     1381 ttccttgatc cccgcggcac tgacaccttc aaccgcacag atgctcttca cgacacttcc
     1441 tcccgggaag atgccccgag accctggacg atgtcggccc gggtgacgat cccgaccagc
     1501 ctgccgtcgc ggagcaccgg gaggcgtgcg ataccttcct ggagcatctg ggcggcggca
     1561 tcggtgatat ccgactcctc gtcnnggtga gcgcacgctt tgtcttctcc cagttgatga
     1621 actccctgac cgggacctcg atcacctcga gcggcgacgg gagccagagg tcgctcgaga
     1681 gatcaccggt atccagaagc gagaggacat cggtctcggt gacgattcct gccacccgct
     1741 cgccgtccat cacgggaagc ccgccgatat ggtatttccg gagcagcccc gccgcctcac
     1801 gcaccggtga gtcgacccgg acggtcaccg ggtccggcgt cataacatcc ttaaccagca
     1861 tcattgtcac ctcaggggag catcccggca ctgcggagac cgtcgtcttc cgcaaacccg
     1921 cacatcaggt tcatgttctg gatcgcctgg ccgcttgcgc ccttgaccag gttatcgatc
     1981 gccgagacca cgacgacccg gtcgccctcg ctctccaccg ccaggtcgca gaagttcgtc
     2041 ccccgcacgg cggcgagggt cggcctctgg tagcggacaa agaactcgtc ctcgtagaac
     2101 ttccggtaga gcgcctccac ctcaccctgc tccatcggct ccgaaaggag gatgtgggcc
     2161 gtcgtgagga tgccccggtt caccgggaga aggtgcggcg tgaagtagca ccgggcgttt
     2221 gagccgagcc gggcgacctc ctgcttcatc tccgcgaggt gccggtgatt cgtccacttg
     2281 taggcggtga agttgtcgcc gacgtttccg tagtgggtgg tcgcggaggg ggaggtcccc
     2341 gccccggaga ccccggtctt cgagtcgaag atgatggtgt gggcgcgact tgcgagcggt
     2401 gccgccgcga gcgtcgcccc ggtcgggaag cacccggggt tcgcgacgaa ggaggctccc
     2461 ctgacctcgt cccgatgaag ttcgggcagg ccgtaggggg ccttgaagta ggcttcgtgg
     2521 gagacaccgt aggtcttctc gaagatatcc tttgagagcc ggtagtcggc cgagaggtca
     2581 acggtcttga tgccccgctc caccagcgtc ccggccacct tcatcgccgc cgtgtggggt
     2641 acggcgagga aggcaaagtc ggcatcgatg tcaccggctt cggtgttccg gaagacgaga
     2701 tcggtcagtc cctgaagatg gggatggacc gaggcgacgc tgaccccgtc cagcttgcgg
     2761 gatgtcgcag cgacgacatc cgcagacgag tggtgcagca ggagccgcat caactccccg
     2821 ccggtgtatc cggatgcacc gataatcgca atttccataa gaaagaggtg tcgtttatac
     2881 aatcttaagg ctttgtggtg tatcgttcgt agagtcgggc ggcgaccgac tcgatctcgt
     2941 cccttccttc gagcccttcg aggagatcct gggggagggc ggttttccca tagacagcac
     3001 ccgcgtaggc cccgcagatg agcgcgatgg tatcggtgtt tcctcccaca tgcgccgcag
     3061 tggtgaggag ggccgtgacg tcgtggatgc ggctgatcag gaagaacgcg agcgggattg
     3121 tctggtaaac ggtcacgtcg ttcccgataa cggataacgc gctctcaagg ctgataccct
     3181 cgtttgccag gcggacagcg tcgcgaatct tgttgccgag ggcaacgtcc tcgaggctgg
     3241 catgcttccc cgcaagggag agggcgtcgg agcgaccgcg gacggcgtgg tggacgagca
     3301 tcgcgacggt gaccgctccc gcatgcgccc ccggatgggt gtgggtgacg ctgcaggcct
     3361 gaaccacccg ctcggtcacg tcgacgggat cgccgtagag aagcccgaac gggacggcga
     3421 tgccggtgca gccggcggtg ttggaggaga cgccgcccgg tctctcgccg gagaggagga
     3481 ggtgccggca cgcggcgtcc acggcaccgt cggggaaccg gagttcctcg ttcatgtaca
     3541 tccgggcaag ggcggccgcg tacccctgct ccgagtaggt gccgtcggcg agcatgccgg
     3601 caacgaggag catcatctgg gtgtcgtccg tgaactgccc gggtttcagc ccggcgttcg
     3661 ggtgcccccg ccacgcacgg cggtatccct cgtggaggcg ctggaggtcc ggcggcgtgc
     3721 tctcccgcgc catgccgagc gcgtcgccga tggcggcccc gaggagacag cccttgaact
     3781 gatcgagcat cttaggtagt atctctctta aggttcctct taagggttgt tgttcgacgg
     3841 cggagaacac cgtgcctctg taccggtgtg ccggaaacga aagcgggcga acgtgggaat
     3901 acgccgtgtc cggcagtcgc tcatcccgga gggcacgcgc gcattgtcgg tcaactgctg
     3961 gtgcctggta gtaccctgac ccgaacgcca ggagcataaa cgtaaaaagg agaacgagga
     4021 gcggtttggt cttgccggtt ccatcaattg cagggattga gccgtacgcg tgtcggatcc
     4081 tggattacac tcactctgca ggatcggata tgttcgcaag cgatatcggt cgaacaccct
     4141 ctcacaaggt cacgaagtat cctttctgcg atatgttcga acgagatagt accccagcaa
     4201 gagcacgatc acggccccca gaacgtacca taggagcacg ggttcgtaga acatgctaaa
     4261 cgcatcctca gatccgtgat acgtcaccgt atcccctatc tcgggctgtt cgagcaacgt
     4321 ttgcgacgca ctccctgcat cgtcaggcga tgcccccccg cccgccgaat cggcccgcac
     4381 atccccaccc gggggcgcag atgcagtcgc tacaccgctg ccgatcagaa cgaagagcag
     4441 cagggcgatg cagattaacc ctccccggcc ggtaccgttc atgactcgtc cgtctcccat
     4501 caatgttcac ctgccggcgc gctccgggat accgattccg gtattcacga gggaaaagtc
     4561 aacgctcttc tataaacctt ttctgtgacg cgcgtctaca cgtcgggcct gcccgatcgg
     4621 tgccacacta gaacgtgacg tagaatgccg ggagccaggg ccgcgccgat aatgaagaat
     4681 acggaccgaa gagccggagc actgcccgct ccttcagggt atcagagttc acccggcagc
     4741 aggatcaacg agaggcacgg gactgatctt cctgattgtg gtacgtgcag atcgagcgat
     4801 ctgtaa
//
LOCUS       DAJB01000172            4824 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_124653, whole genome shotgun sequence.
ACCESSION   DAJB01000172 DAJB01000000
VERSION     DAJB01000172.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_124653"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2612..2770
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          897 a         1461 c         1488 g          817 t
ORIGIN      
        1 accatgcttc cttcggtcgc ggggggaaca gtcgagcgct acgaccgcgg cggctacacg
       61 gtggtccacg gggatcaggc ggtcgacgac cacgggacgc tgatcatcgg ccacgaccac
      121 ccggcgatcg agatcgatat ggcccggttc ccctgcttcc tctacgggca gggcgcggtg
      181 cgcgggaaag accttatcgt gatgccggcc gcgaacccgc tctcccccgg cgtcatcatc
      241 aacgccgcca ggagccagga cttcctctcg cccatcctcc gccgcgtgga cgcaggaagc
      301 ctgcagccgg tggtggaggt ggacggcgag gcggtggtcc tcccgccgct tgccggcatc
      361 cggcggttcg cgtgaagaga tatcaggtga ccttccggta cagggcgatg tcgagcgggc
      421 cggcaaacag gggtcctgcc ctctccccga agcccctgaa gtgcggagtt ccgttgtggg
      481 cgtcgattgc ggcccggtcc ttccaggtct cgatgaacgt gaacttcgcg gggttcgcga
      541 ggtccacgta aaggtcgtag gagacgttcc cctcctcgcc ccggcttgca tcgacgaggt
      601 ccagggcaat cttcatgaat tcatcgatct tttccggtct caccgtgcat cgtgcaacga
      661 tcgttatcat annggaacgc tatagcgccg gggctcaaac agggtcccgg gatcattgtg
      721 tggatcgcaa acatcctcgg cggaacggtc atactcttcc tcggaatcat catccgggtc
      781 ttcaacgcga gcggcctcat tgcaggctac aacaccgcgt ccgcggagga gaaggcgaag
      841 tacgacgaga gagcgctgac gaagttcgtg ggcggcctcc tgattgccgc ttccgtgatc
      901 cttctcgcac gcgggatcct gccggccgtt gcaggaatat cgtgggccct cttcatggcg
      961 atcgtcatcg ggggcgtcgt ctacatgaac accggggagc ggttcaggtg gtgagggagt
     1021 ggggcgctcc gcagctcagg caggccccgc aaacccggcc gccctctctg catacaccgg
     1081 ataccgggcc gagaagaccg ctccgccaaa gatcgggagc cagatgcaga tatccgtgac
     1141 gatccccggc gctcccggat agaggacgta gagcacctcg ctcgcaagcc cgatgacgaa
     1201 catcaggccc cagaaggccg tcaactgacg gttcagccgg atgaagaagg gatcggtcca
     1261 ctcctcctcg gaaacctcgc gccgtgcata ctgctcggtg aacggtaccc cgagaaggag
     1321 gctccccagc gtgccgagga ggagaaaccc gttcgaggcg atgccgagga gcgggacgat
     1381 atcgaaccag cagagggcca gcacgaggac ggaaacgacc gcaaagaaga gcgtcgtcac
     1441 cccggcgatg atgaaccggt ccttgaggtc ccggtagccg acgacgatgg acgcggcgag
     1501 cgagaggagg aggatgaact cgatcggcac cggggtgtag acggcgaggg ccgcgaagat
     1561 gagccagggg gagaaggaga cgagaaggtc gagcgtcttc atgggggcaa cagatcctgt
     1621 attgaggggt tccggatgtc cggatgaagc ttttgaaatg aacccgcatg agtaacgcct
     1681 atatacgctc ccgtcctccg gggtcccgga gttggccctc atgagacgcc ggactgttat
     1741 catcctcgcc accgttgcgg tgctcgccct gctcggcatc gccgtggccg tcgtgatgcc
     1801 gccgggcggc accaccggca ccctgccgct cgatacgatc accctgcctg cggggtttgc
     1861 gatcgacgtc tacgccggga acgtcaccgg ggcacggtcc atgaccctct ccccgaacgg
     1921 gacgcttttt gtcgggagcc gcggtcccgg gaacgtctac gccatccccg accgggacgg
     1981 tgacggtcgg gccgatgcgg tgatcaccgt cgcgagcggt cttaactccc caaacggggt
     2041 cgcgttccgg aacggctcgc tctacgtcgc cgagatcggg cgcatcctgc gctatgacaa
     2101 tatcgaggca gacctcgaga acccgcccga ggcggtggtg gtgagcgacg ccttcccggg
     2161 tgatgcgtcg cacgggtgga agttcatcgc gttcgggccc gacggcaaac tctacgtccc
     2221 ggtgggcgtg ccctgcaacg tctgcgaccc cgacgacgag cggttcggca ccatcctccg
     2281 gatgaacccg gacgggaccg acctcgaggt atatgcccgg ggcatacgaa acacggtggg
     2341 attcgactgg gacccggaga ccggcgattt atggttcacc gacaacggcc gggactggct
     2401 cggcgataat atcccgccgg atgaattgaa ccgggcgccg gaagcgggga tgcactttgg
     2461 gttcccctac tgccacggac ggtcgatcgc cgacccggag ttcggggaag ggcagtcgtg
     2521 cgcgaacttc acgccccctg agcaggaact cgggccgcac gtggccgcgc tcggtatgcg
     2581 gttctatgac ggcgagcagt tcccggagga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn gacgaccggg cgaatgccat ctaccggata acctacccgg aggtcacttc
     2821 cccttccgga tgacccggcc gaggtggaac ggcgtcgggc ccggtgcgac ggcggggaac
     2881 gtcccctccg ccgcgcgccg gacatcctcg agggagtaga atgccttcgg gttgaactcg
     2941 tggatggcct tctccacgtc cttgatgttc ttcctcttaa ccaccgagaa gattaccttg
     3001 ccggggccct gcttcccctg ggcgtcgagg actgtcactc cgtagccggc ggcgcggagg
     3061 tactcgataa ggttggtcgc atcccgctgg gtgatgatcc ggacgagggc aagccccatt
     3121 gccagccgct cctcgacgat catcccgatg tagttcccgg tgccgaaccc ggcggcatag
     3181 gcgaggtagt tgagcgggtt ggtgaggttc tggaagatct ggccgatggc gatgatccag
     3241 atgaagacct cgaagaaccc gagaaccgga gcgatcatct tcatgccccg ggatatgaag
     3301 atgatccgca tcgtcccgat ggtgacgtca catattcgcg caaaaaaaat gaataccggg
     3361 actatgacca gcgagaagac ctccgggtcg atatccggaa cgatgccgag catagatcat
     3421 gattgatgag caggtgttat atttttatct atattcaaat atatttcgaa cgcagaagag
     3481 ttccaggaac ctttctttag aggaattaca cgaagatgcg ctggaaaaag gtcgaaagac
     3541 acacggatca taggctgatc gtggcgtccc attcctctgc ccggaggttc tccggcctct
     3601 tttcgctccc ccggagagag ctccttcccg cagaaagggc tgacaccggc cccccgtgtc
     3661 cacgggtccg gtagcatcgc aaaattaccc ccgtccatcg ggtacttcag cccgtagccg
     3721 gagtttcggc cggagccccc gttggatagc cttatgatat cagcgtccga tcaggcatag
     3781 tgtggtgaga taccatgcca aagtgctaca gttgcggtgc cgagatcaat ccgaagaaga
     3841 cgagatgtcc gaaatgcggg gcaaaggttg aggcagggag cggaaaacaa ccggaagccg
     3901 gagttgttgg ggggcggcgg taaaaatccg cgaaaacccc ctcaccgaac ctcttctact
     3961 ctttcctggt ctttttccac ccggtagccc ggggatgtgc gccaccagta gtagacttcg
     4021 tagacgagca gcgcgagcag caccgcgcag gcgccgaaga agaagcccat caccaggtca
     4081 tggaccggcg gcgccccggc ggcatcgagg gtgggggcct ggagcgtcga gaactcctct
     4141 ccgttcccgc cggcaaccct ctctgcgggc gccgcgtccc aggatggcgg cagcaccgcc
     4201 ggaagaaccg cccagagccc gaggctcgcc aggacaacga tcccaaagag tgtcgcgtac
     4261 ttctgcagca ccgaccggag gtcgcttacc ggcgacgcga tgatgaggac ctggttcgta
     4321 aggccgtaga ccttcacctc gcgtcctttc tcgctccagc gcgtctccat aacctcaaga
     4381 agcccgacgt cgagaaggtt ctcgatgtgg taggaagcgg tggtgatggg gatcttcatc
     4441 tgccgggcga cctgcgacga cgtcagcggc ccgctaccga aggcctggat gaccgcgttc
     4501 gccgtctggc tcgccattgc ccgggcgatc ttctgtgccc gctcatcgcc cggctggatc
     4561 actacaacat cgtcaaccat ggagtgcttc aagaatccgg ctgtgcccgt gctcgagcgc
     4621 ctcttcgagc cgggaggcgt ccgagcctgc gccctgcgca aggttcggct tgccgccgcc
     4681 cttcccgccg aggacgctgc aggcctcccg cacgatatcg gcagcgttca ccgccggtat
     4741 tcccgacgtc gccaccacct tcacgtttcc gtccgccgat gcaaagagcg ccacgccgcc
     4801 ctcatcagcg acggtggtgg cgag
//
LOCUS       DAJB01000173            7717 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_60292, whole genome shotgun sequence.
ACCESSION   DAJB01000173 DAJB01000000
VERSION     DAJB01000173.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_60292"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2485..2532
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7164..7201
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1463 a         2402 c         2445 g         1321 t
ORIGIN      
        1 ggttgtacga ctacatcggc aaggaggcga tggcgtgacg ggaaaggtcc gggatgcggc
       61 aatccgtatc aaactccagg agatgaccga gagcgtcgat ctggtcaaag agcacctccc
      121 cgactcagcc gattcgttca ggcagcttgg catcataaaa gacggcgtat acaagcgcgc
      181 cgaatatgcc atcgagaacg tcttcgacat ctgcgcaatc ctgaatgccg acctccacct
      241 cggcgtcccc ggcaccgatg aggatatcct cgagaacctc gtgcagcatg gggtgctcac
      301 ccccgggatg cggaagaacc tgaaggcgat gaaggggttc cggaacattg tcgtgcaccg
      361 ctacggggca atcgacgatg cgcttgcctt ctcaatcctg gaggagcata tcggcgattt
      421 tgctctcttc aggcaggagg ttgaacactt cctgcgggcg gtgagtggaa gatgacgggg
      481 ccagagagtg cgatagttcg gaggacctgg aactcaagaa gggccaacta ccccctgaac
      541 ccggcgatca acgttatgag atcgttatga gattggggct gcaatcgcag ggataccatc
      601 ccgggatgcc cacgacaaaa ggagggggga ccagccactg cacttaccgc gatttctcgg
      661 gaggctgttg ctgcatctga accggcattc cctgtagttt gataaatgac ttcccgttct
      721 ggttctcaat aaccttcacc gcataggttc cgccgaaggt gtgcgttgtc gcatgataga
      781 gcgaaagaac aaggtcctgg aactgttgat cgtcctctaa atcatccaca tacagaccaa
      841 tctctcgcgc ctcggtacgg ctgatatggt gcccatggct tctaaacagc gtgtggttgg
      901 agagacgatc tgcgatagtc tctgcagatt ctcttgcctg ctccggatca ttatgatctg
      961 cgaacatata ccgggccagc catgtagaga catacttctt cgatagcgca atcgcgttct
     1021 gggactggac aagtagagca ggaccatact ggttcaggat cggaagccat gcgccaagag
     1081 tccggggatc cttacactgg tccttcgcca tatcgaactg atcaagtatc gcctgtgcag
     1141 gaactgccat caccccaagc ggggtttgga tgatgaattg tggatcgatc ggaccgacaa
     1201 acgaatgctt ccccataaca atcctattcg acgcacaggc gatcatcgtt gcagccgaca
     1261 tcgccgcatg cgggataata acgcggatgt cgtcaaattt cgagcggaga taggcaacaa
     1321 ttgctgcagc agcttccggc gaacctccgg gactatgtaa aataagatcg agttctttcc
     1381 catgaagccc gtgaattact tccatcagcc cttggatatc ttcttcggtt atggagatgg
     1441 cattcgggtc tttcccgggc tgggtccagc tggttgcata caggatacag ttccgtccgg
     1501 tatacgatga gagactgacc agatactttc gccgaacata atcgaaagga ggctcgttcc
     1561 ttttaacgaa tgcttcctcc agttcctttg cgatcccgct ccaggtcggc ataacagtgc
     1621 gtcaggttga ccgggttccc gctgtagacc gggcaaacgg cttattgcct tcgacatata
     1681 cccgcgtttg ctccataacc tcatcgtatc ttccgtaagc ccgcatcagt tcggtaatcc
     1741 ccggctgtgc ctgtgctctt ctgatgagtt cctccgcctc cgcctgaaaa tcagtcgcca
     1801 ccgcatctca ccccgatttt tgaatatact acaatctggt tacgattagt tatactttac
     1861 gacgagcagg ggcgacccca cggtcttcgc gtgaggcaac ccgggagcaa aaggtgatgc
     1921 gccttcgcag gaagtaagat cgttcgatac catggaagca tccatccggg acggattcac
     1981 ccggcagctc cgtaaccgta acccctcgaa cctcatttct cacgcaagca gcgccgcgat
     2041 gatcccggtc aggaatatgc cgtcgaacgt ccccgccccc ccgatgcttg ccgtcggcgc
     2101 tcccagctcc gcgatatggt ggaggttcaa gaggtcggcc ccgacgagag tgccgagcgt
     2161 cccgctcacg taggcgatga tcaccgccgc agccgggaca cctcccgcaa agagcgagag
     2221 gatgagcgcc gcaaagagtg cggcgagcgg cgggatgaag aacggcgtcg caatcccgag
     2281 gcccggcacc gggcgggcga cgagcttcgt gatgacggtc acgaaggcga ccccgaggag
     2341 tgcgagcccc agaaccaggt agccgccgct gatcacgacc gagtcgtaga gcaggtagag
     2401 cgatatcgcg agtggaatga gggcaccgcc gacgttgatc gcgaggaccg tccgcggcac
     2461 ctgctgcggg atacggtaga gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnaggaacgc ctgccaccag gagaacccga gcttcgcaaa cgtcgccccg
     2581 atcgccgtca ggaagaggac cgggaagacg atgacggcga gcccgaccag gatgagcagc
     2641 ccgaagaaga tgaggaggac aagaggagag aacgggttga agacgactcg gtccatgccg
     2701 gaaggtgctc ccccatctat aatatatgct ctcaccggaa ggagcagacg atcgcggcgt
     2761 ggtcgtggtg gtagggctcg agccaccgga cgtccgtcac gtcgaggcgc cgttcgagcc
     2821 cggcccgcgc ctccgagagc acctcggccg ggtcccgccg gacgtccacg ctccgggtct
     2881 tgagcatcag gatgaggtgt ccaccgggct tgaggaagac gaggttccgc tccgcgatct
     2941 cgacctggtt gggctgtgcc acgtcctgat agaccagatc caccgcctcc atgaacggtg
     3001 catactcgtc cggccggttc gcgtcggcca tgatcgggac gatgttcctc ctccggcggg
     3061 ccacctcgag gagatcctgc atcggccggg gggcaaactc caccgcgtag acagtctcga
     3121 cgtagtcggc gacgtgtgag accgtggtcc cgttcgccgc accgaggtag agcacccgca
     3181 gatcgggggt gagatccatg ccgccgccga gcacgtagag cgccgcgagc ttgctccggt
     3241 aggggtccca gacccggtag ccgtccagcg tccgctcccc gtagaccccg ccttcaccgg
     3301 gtgagacgag cacgtttcca agccagatca tgccgcgacc cccgcgttgt cgatccgcgc
     3361 ctgggcattc tggatgaact ccggcaccgc ctctccccgg tagtagtcga gccgggcggc
     3421 gatggcgagt ttcgccgcga gcacccgggc cacccgcccg cgcacgtctt tgggggcatt
     3481 gtggaccctc cggtgctgga agatgatccc gtgcttcggc ggcggcgtcc cgccccggag
     3541 gtgcgagaag agcgccctct ccgagccaag gacctggatg gtgctgcccg gcatcctcgc
     3601 aagtgcagag agcccccccg cccgggagag gagtcgggcc gcgaccagcc cgccgatgag
     3661 ggcgctcgta ttcggcagca cctcgtcggc ccgggccgag acctcccgca tcaggcgggt
     3721 cctcgtcccg gcgagccgtt cgatctcgtc ggcagcgtcg gagagaccgc cccgggcccc
     3781 cctccggatg aggtcgagca tctttctggc cgggagcgac cggtacttcc gggagaacga
     3841 cggagtcgtg acctgatacc actctgcggc gcgttcctgg aggaggttga tgacgttatc
     3901 gagctcgtcg agcatcctga ccatctggag gagctcgacg tcccggccct ggtaggagag
     3961 ggcgatcttt tcccgggcaa gggtggtgca gaccgcacga agcaggttga cgtactcctc
     4021 ccgtgtccgg acgaccccgc agtcccgggc aacctcccag tcgatgggaa tataagactc
     4081 cataccggtg cggagcgtgg cggcccgctc tgcgagcgcg gccggatcgg cgccggcagc
     4141 ccggcactcc tgctcatcga catctccgaa ccagtaacgc tgtagcatgg tacggtattg
     4201 gtgcccggcc gatataaggg tgaaccggac agcaactgat atccgggagc cgcctcatac
     4261 cctgcaacca tgctctcgca ggtaaccgcc tccctcccgg agttcgtcgt cggcatcctt
     4321 ctcctcgtca agggagccga cttcttcgtc ggcggaggga gcgggcttgc cgcccggtac
     4381 cgggtctcgc cggcgctgat cgggttcacc gtcatcgcgt ttgggacatc cctcccggaa
     4441 ctcgtggtga acctccaggc tgccgcggca gcggagcccg acctcgcgct cgggaatatc
     4501 gtcgggagca ccgtcgccaa catcgccctc gtcctcgccg tcgtggccgt cgtcaacccc
     4561 gaagcgctcc gggaagaggg ggcgcacgga atgagacagg tcgccgtgat gcttgcggca
     4621 gccaccctct tttcgcttct tgccctccgg ggagtcttcg accttcccgc cggcatcacg
     4681 atgctcgggg ccttcgccct caccatggta gtcctgtggc ggcagggggt ggccacagag
     4741 gagacgtttg agagccaggg catccgcgac gttctcctca ccggcggcgg gcttatcgcg
     4801 gtcatcctcg gctcctggct gctggttgaa tccgcaaccc ggatcgccgt cgccttcggg
     4861 atcagcccgt tcgtcatcgg cgtcagcatg gtcgccgtgg gcacgtcgct cccggaactc
     4921 gccacgtccc tcaccgctgc ggtgcggggt gcaggcgcga tatcggtcgg gaacatcgtc
     4981 ggcagcaaca ccttcaatgt cctcttcgtg atggggtgcg cagcgatcgt cctgccgata
     5041 ccggtgggct cgtatgccaa cgtcctcgtc atgctgggat ttgtcgtcgc catcctgccg
     5101 ttcttctcct cccgggtgca ggtgcggcgg gggtggggac tcgtgatgct cctcgcatat
     5161 gccggatata tcggcctgct cttcgggctt atatgatccc gaacgagtag accatcacgg
     5221 ggaccatgat cgcgcccatg cagtagaccc ggcggatgtt gacgagagtc ggcacatagc
     5281 cgaccgccgt tgccaggagg aggataagtc ccccgaacgg gccgcagaga aagagggaga
     5341 ggaggacgac gaaggcgatg acggcgtagt tgaggttcgt aacgttcacg ccggaaaacc
     5401 accccgccgt cgacgagaag aggatggtga ggaggtaggc gccgaccgcg gctatcgcgc
     5461 ccgcaaggag aatggcggtg gccggcggca tctcctcgag cgccgatatc gccgccatca
     5521 ccccgctccg cgtccgggat acggcgtaaa acgccgcaag cccgaggaag gcgttcacgg
     5581 tgttcgcggc gctggtcgca aggatatact cccggggatt ggagtcgtag ccgatgaccg
     5641 aggtcaggag ggcgtttgcc gtggcgctcg agagacccgg gagccagccg accagggcgc
     5701 cggcgacgga gccgaggagg gaactccgcc ggacggtacc ccgggggagg tcgatacccg
     5761 agaagtgctg tgcgggcatg accccgtgcg acgccttgag gaggaccgcg atcccgaaaa
     5821 gcccggagag gaggggcatc agcaccccgg actcgccgcc cgccggccag gcgaggaagg
     5881 agtagccgag ggagaagagc ccgaggatcc ccgagaccga aaagaccgcc agcgcccatc
     5941 ccggcgactc ggagacgacg atgaggtagc ccgccaccgc gaggatgacg aggccgatac
     6001 cccagtcgat cgccggctgg agggcgggga ggaccaggac aaaggccagg gcgagcggga
     6061 gggagagggc gacgccggcg gcgctcccga gagccgatat ccggacggcc tcctcacccc
     6121 gcccttcgag gcagagggcg tgcgccggga ggaccgcaag cgacgtgtcg gcgtcgggga
     6181 tgccgaggaa ggtgctcggg acgttatcga gaaacgtgtg cgtaacgagg gtggaaaaca
     6241 tcgcggaagc gacgactacc gggtcgaacc aggcaagcag gagcgcctgg agcgagagga
     6301 ggacccctgc catggtgttt gcgtggatgc cggggacgag gccgctcacg gcgccgcagc
     6361 cgatgccgat ggcggccccg agcacgatac tgaagagcac gttgatctac ggtcgaagca
     6421 ggctatgata aaccaaccga atcgtgtccc acgatacagt catattccac cgggacgaag
     6481 gttgtagcga tgaagattgc catcacccgg ctcgagaaga aggcggcagg cgaccatgcc
     6541 ctctgcgcaa cttatggcca cgattgttat acggtctcgc cgctccgggc ggaacttcgg
     6601 cctgagcggg tcgctctgtt cgtcgaagcc gtccaccgcg gcgagttcga ctgcctcttc
     6661 ttctcaagcg ccctccctgc cgcgattgtc gggccgaaac tcgaccggtg gccccgggtg
     6721 atcgcgatcg ggcccaagac cgccgaagtc ctcaaggagg ccgggatcga ggcggaggtt
     6781 ctcccctcgt tctactcccg cgacttcgtc ccctacctcg gggactggct ccggggccgg
     6841 acggtcggga tcccgcgggc cgacgtcccg aaccctggcc ttctcgacgc gatcgccgct
     6901 gcgggaggga cggtccggga ggaacgggtc tacgcgctcg tccccacggg aaccgaactc
     6961 gcgctcgata ccgccaacgc cgtcctcttc acgagtgcca tgtcctaccg ggaggcccgg
     7021 tggcaacccc gcgacgacct catcctcgtc gccatcggcg atatcaccgc cgaagcgatg
     7081 cgagttggtg gacatccccc tgcggtcgtc ggggacgggt cgcttgcagg aaccctcgag
     7141 gcgctgaacc gccacatgga gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nctcgacccg caggtctccg gggtgctcat tgtcatgttc ggcggcgccg tccggctcgc
     7261 ccccgtcctc ctctcgcaca acaaggagta tgtctgctta atgaggctcc acggcgacgc
     7321 atcccgggag accgtggacc gggtggcgga ggagttcacc ggccggatct accagcggcc
     7381 gcccaaaaag agcgcggtca aacggagcct ccggatccgg aagatccagg agatcgagat
     7441 cctggatatg gaaggcaggc ttgtcctctt ccgggtcagg tgcgatgccg ggacctacat
     7501 ccggacgctc tgcgtccaca tgggctatgc gatcggggtc tgcgcccata tggaggaact
     7561 ccggaggata cggtcgggat cgttcgacga ggccgccgcc gtaacgcttc acgagctcgc
     7621 cgatgccgcc gcagcggccc gggatggcga gccgggcccg cttcggcgga tgctcctctc
     7681 ccctgacacc gccatcggcg acctcccgag ggtcgtc
//
LOCUS       DAJB01000174           27833 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_45133, whole genome shotgun sequence.
ACCESSION   DAJB01000174 DAJB01000000
VERSION     DAJB01000174.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27833)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27833)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27833
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_45133"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    20675..20711
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23577..23626
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24007..24026
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5138 a         8470 c         8336 g         5775 t
ORIGIN      
        1 ttgcaatcaa tcgggaacac ccggatgcga tctcaacaaa gttattatca gttgtttcgt
       61 agggcactac cggcacgggg gcaagcgacc cccgcaaata cgataaccac tacggcaacc
      121 gaccgtgcgg cctcaccctc ggggagaccg tgacagccct gacccgcttc ttttgcccga
      181 cactctccgg acactttagg aatcgccagg tgaacctgcc cgcgttgccg gaactcagat
      241 cgagtccccc ggtccgtcct ctcatcccga tcaatcgggt gcgcatttat aggaggggac
      301 gacaataggg ttctatgccc ccgatggggt tgatctcccc ctcgtacatc tatgtctgca
      361 cccggttccg ggtccgcagg acgacactcc ttccccgtga agcgtacctg cggatcatgc
      421 ggatgagcat accggaggtt gtcgcccacc tcggccagca ggagtacgga caggagatca
      481 tggatctcgc acacgacttc gccggcgccc agctcatcga agaggcggta aaccgcaacc
      541 tggcgcggtc gttccggcac gccatggcga tcaccccggg gaacctccgg accctgaccg
      601 gggaatacct caaccggtgg gacatcgcga acatcatggc gatcctgcgg gggacggtcc
      661 acggcatacc ccggcggcag gtccgcgacc tcctcatccc ggcgggcgag gtcgacaacg
      721 tgctcctcga ccgcctcctc ggtttcacga cctgcgaaga gattctcgag gcactccaga
      781 actggcggct ctacccggtc cttgcggaat actaccggac ctgcggagag cagggcatct
      841 ttgcccggat cgagaacgag ctctaccggc agtactacgc cgggctgctc aacctcggca
      901 catccgggtg cagcggatgc caggaattgc tcgcctatct ccggttcgag atcgatatca
      961 ccaacatgga gaacctcatc cggcttcact gcggagaaga agcatgcgat ctcacgctca
     1021 tcgaccagac catgatcccg gggggccgta tccccatcgc cttcttccgc cggctctacg
     1081 gcatcgagac ggagggagag ttcgtcaacg tcttcctcca gaccgatatc gtcccggttc
     1141 tggcccaggc ggtgcgggaa ctccggcagg acccgggctt ctccagcgtc gacgccgcgg
     1201 aactggtctg gcagcggtgg caccagcacc ggcggcccat tcacgagatc gaaatagcgg
     1261 tcacccgcat caggcttcac cacttagagt ccctctcccg gcgtcatccg ttctcggtcc
     1321 tgccggtgct tgcatacctg gaacggaaga agtacgaggt cttcaacctg cggacaatcg
     1381 cgcgaggaaa agcattcggt ctgccgcccg agcgaatatg gcagtatatc gtcctgtagg
     1441 tgagtatcat gccggttgac gatatcaaac ccacccggtt cggcctcctg atcgtcaggc
     1501 ggcggatggc gctcgccgag cggatacacc gactgttagc gatgaagctc gacggcatga
     1561 tgctggaact ggtcagactc accgaccaga ccgcccgaga gcgcaggatg ctcgaagagc
     1621 aatacagccg ggcacgagag atggtggcag tcgccgccat gatggagggg gctacggggg
     1681 tgctccttgc ggcgctctcg gtggagacct cccccaccta cacgaccggg cacaggaacg
     1741 tctttggggt acgactcccg gacctcgaac cggtgatggt caaaaagacc ctcgcccagc
     1801 ggggttacgg catcctcggg acctcgtcgg tcattgatga cgccgccgac gcctacgagg
     1861 accttcttat ggccatcatc acaagcgccg aactcgaagg agggatcaaa cggctgctcg
     1921 acgatatcga acgaacccgg cggcgggtga acgcgctgga gttcaaagta atcccggatc
     1981 ttacagagac acttcggttc atcgagaatc agcgcgacga gatggagcgg caggagtgga
     2041 cacggctccg gcgtatcaaa aaaataaagg taaagagagc aaggagcccc tagagggcgc
     2101 cggcttgctg cttctgcagg cacgcgattg cggcatccac aatcacggcg agggcatcca
     2161 caccgaaggt ctcggacgag aggacgatat catacgccga gaggtcatcg atctcgatac
     2221 cgtagtagtt ccggtaacgg atggcttccg agcgctgccg gttctcagtg tagacccggg
     2281 ccgcttcttc gtccatcccg tcacggcccg ctatccgctt tgcccggcag gagatcgatg
     2341 cagagagcca gatccggaga tcggcgttcc cgaccatccg gcccgagagc ctgccctcga
     2401 cgatgatgtt gccggcgccc tcaccgatct ctttctgccg ggcgtctatc atccggtcga
     2461 ccgcgggatc gctctcggcg aaccttccga actcggcgag gtccatgccg tgctcccggg
     2521 cgagctggcg gaagacctct cctgcagaga tgaggtcaag cccgtacttc ccggccaggt
     2581 accgggcaag cgaagtggtg ccgctccccg gcaggccgct gatagtgatc cgcatcaaag
     2641 ccctccgatg ttgagggcct tccggatcac ctggctgacg gtcagcgagc agatcatgta
     2701 ccagatgatc caggcaggga cgatccagaa cgcgaagtcc gagaggacga cagttccgac
     2761 gaagggcaaa acgataccgt tctgcaggct aatggcggtc gccacgccga ccggcgggag
     2821 ccgcaggagg agccagaaga agatcggcac cgacaacagg aggatgatcg ccatcggggt
     2881 aaactgctgc cgggagatgt cgagctggtc ctgcatcatc cggtcctgtt tcccctggag
     2941 tttcttgacc ctcttttcat caccggagag ctgggcctcg cggaactcct tctggaacac
     3001 ccgcatcttc gcctgcgtat cctgcatctt ctcgtaatcg atggtatact tctggacgag
     3061 ggacgagtac aggcccgtcg tgccggagag gatcaggatc atgatgaaaa acggcacgcc
     3121 aaaactatcg atgaaggggc ccagcactac attcatcgcc gtaccgaccg tcacccgtat
     3181 ccattcgacg ctgtacgaga gcatcacgag catggtaaag atgagggcga tcgtcggccc
     3241 ttgcttcttc aggtccacca tgtcaggtta cctcagcaca cccacaaggt cgtcgatgcc
     3301 tcgttcgagc aggaagtttt cgttcctgac gatcttgatg gtgcagccgg tgtacattgc
     3361 atatgccgcg gcaacggcgc ggttgaactc ctggtgctcg gtgatcgcgc gggctccttc
     3421 catgtcgcgg acccgggaat catccccgag ccggcgcatc aggatctggt cagggtcggt
     3481 ctccaccagc acgaccatat cgggcatcag ttcgcggagc acccattcgg gcagccccgc
     3541 aaggaacccc gtcggggtct tgacgctgct gtgcgtatcg atgatgatat ttgcatcccc
     3601 actcatggcg gcgatccccg acgcagccgc ctgctggagg cgtttctgtg catcttttcc
     3661 aagcttgcgc atttcatcgc gattcgagac caggttctcc ttctgggcta cgtcaaacat
     3721 gaacgtaccg aagttgacgg ccgtgtagct aacgccctgg gccgccagtc tctccatcgc
     3781 accgttgatg acggtggtct tcccaacgcc gggaaccccc gttacgacga ctttcttccc
     3841 caactctatc actccatgta gaatatagtt cagtgtaaaa taaatccgcg cctgaaaagt
     3901 aagtcctttc ggaatcattc cgggctgaac gatcgcgagg aacgtccggg tggtaaaacg
     3961 gatgttgtgc ggtttttggg aaaagagagc cggatgggct cactcccggg aggagagtgg
     4021 gcctccgaac ttactctttc ccaaagaatc cccgcatgaa cggatacatc tccatgatct
     4081 gctggcttgc gatctcctcg tagaggcggt aggtgatact cactgcaagc agcagccccg
     4141 ttccaccgac cgcaccgatc acaccaaaga ggttcgcgac aaccgagagg aggccgatga
     4201 acacaccgcc gatgacggtg atccttggta tgtagcggtc gaggtacttc tcaagcacct
     4261 gttcgttccg ccggtagccg gggatctgca tcccgcttct ctggatctgc cgggccacgt
     4321 cctttgagtc gaggcctgca gtcttgaccc agaagagcgc gaagatcgca cccccgacca
     4381 ccataacggc gatatcgatg cccatgcgca tgaggatctc ccagggggca tgcccgagat
     4441 cggagagcca ccacatccag tcatgcggcc ggttgatggg agcgatatac cacatgaggc
     4501 cgttgaccgg cgtctgcccc tggaacgtgc cgaggatcgt tatcccggcg ttcgagagga
     4561 acatcccgat catctggatg ttcgcctgca gcacccgaac caggatcatc ggcagaacac
     4621 tcgcgtagat gagcttcacc gggaaccgcg cgcgggcgcc gcggacagcg gtatgcgcga
     4681 gcgggatctc gatacgggtc gactccacgt agacgatgac catgaagatc gcgaccgtcg
     4741 taacgagggc aagcatgtcc gtgccgaaat actcgaggaa gtttgccccc gacgtaccta
     4801 tggcgaacag ccgcgggaag aacccgatcg ggaagggatc cgtgcccgtc tgccagttca
     4861 ggaaaccgtt cacgaggccc tgcgagactc ccgcaacgat gaagagcccc acacccgaac
     4921 cgacacccca cttggtgacg acctcgtcca tcagcaccac caggagtccg ccgaggcaga
     4981 gctggaagaa gatcaggagc gagaccgtga gcgtactgcc gccgaagaac tgcgttgcca
     5041 ccgacgggtc gggcatcatc agcccgctcg cgaccatggg aagcgcttcg acgatgatca
     5101 tcacgaagat gagcagtttc tgcaggccca tatacatgac ctggccgcgt gcatcgctgg
     5161 tatcgatctg caggattccg gcgcccttga gcaactgcag cacgatggat gcggtgacaa
     5221 tcggcccgat accgagatgc agaagcgaac cgcttgcacc ggcaagcagg gcacgatata
     5281 ttccgaaaat atcctgtgac tccggagaga gcccgaagat atcgatgttg gtcaatgtga
     5341 agtagaggag cagaatcgca agcgtccaca taagtttatt cttaaaatgg acgtgcccct
     5401 ccggacttcg gactgcaggc atcgctgcaa ggatgggttc aaatctatcc agcagatctc
     5461 ccatggtttc acctaaaaaa ggagatcaga cggtcgttgc ctgaccgccc atctcctcaa
     5521 tctttgcccg ggcccgttct gaaaactccc gggcggagat gctcatcttg tgggtgactc
     5581 ttccgccacc cagcaccttc tcgatgccga tttcggtagc atcgagggca atgaggtctc
     5641 cttcctggct tgcaagcccc tcacggagca gtgtctcggc catctggtcg atctccccga
     5701 tgtcaagagc ggataccggc acggaagtct tgctgacaaa accgtgcttg ccgttggata
     5761 tctcgccctt caggtagaaa tgcgagaagc ggtggtcccg ctgcccggct ctacctcgtc
     5821 cacccctgtt gccagcgcca cgccggttct tgtgcgtacc gccaccacag gtccgtgaac
     5881 ccctatattt cgatcgcttg tttacgggca tcagtctcac ctcatcttgt agaggagatc
     5941 gttgatctgg gggccataat agccgagagc tccaccctgc tgaaaggttc gcttgatagt
     6001 tttatagccc tttcgtggcg ggtgcagtct taacaccggc ttgatctcca ccagatcgcg
     6061 aaaactcgtc tcaccgctgc agagcgctgt tgcgaattca tcgatgtcgg catacggggt
     6121 atgcgcacgg acgtagtcgt cagtcagctg gtcgttcccg gtcagtctgc cccgggtgtg
     6181 caggagggtg gccagggtct cccggtccac ctcgccgtac gccacgtagt ccttcacctt
     6241 gcgaatcatg cccaggtacg caggcgtatc gggcacgagg acgcagtggt tgacatggtg
     6301 cagacgaagc atcttgaggg tgtccttgat ctcgcggttg gtattgacca cgccgcgcac
     6361 ctgtactacc gcgtacatca ctctgtccct ccgattctga cgaaattcgt ctgcttgagc
     6421 gcctcgaagg tcgccttcgc gtagttgatg gtcgtccggg tctgcccccg gttgaaagcc
     6481 cagacatcct tgatgcctgc aagcgtcagg accttctttg gggtctctcc ggtcacaaga
     6541 ccgatcccct gcggagcggg cttgagcgtc acccggacgc tgcctgcctt cccggtcact
     6601 tcgatcggga ttgagtgccc ggtatcgcaa ccgcactccc agcttccgca tccgcgggag
     6661 actttgatca ggttcagttt tgcgtttacg attgccttct ggatcgcatt gccgacctgg
     6721 acatcctttc cctggccgaa cccgacgtag ccgttccggt tgccaaccac cacgacggtg
     6781 cggaacttca cacggcgacc cgagtcggtc attcgctgaa ccatgttgat gtccagcacc
     6841 tcgtcctcca ggtcgggcag gaggagatcg acgatctggg gctccttgat cggcctgccg
     6901 cttgcgagca cctcatcgat actggtgatc tcgcctgcgg cgaccatgcg cccgagaccg
     6961 gtgagcggaa tccattcttc ctgtacgtat gccatgtcag accagctcct tcttgatggc
     7021 cagagccaca gcctctacat tctctaccag gttacctgcc cgctcgggag catacctggc
     7081 aatgtgggca cccttgagcc gttcctcatc ggggaggatc gactcgccat aggggacatc
     7141 gagaccgcca tccactgctc ccttgagagc ggcaaatacg cgcgctccgg gctttgcccg
     7201 ggcgagtccg atatcaagga tactctcctc gtatcctgca ttcaacgcct tgaccgcgaa
     7261 cagcattccg gtcaggtagg cggccggggt gctggaggtc gatccctcgt agccgtaatc
     7321 ggcaagttca gccgagtatg cagctaccag ggtgcggtct ccctcaatct cggggacgac
     7381 cagctgtacg atgatctgcc ggttcgtcct tcggacgacc attctcggga ttcctgatga
     7441 caggagcgac atccgcttgt agtaatcagt cttgccctcg tgccgccttc ggaagggcac
     7501 aaagtatctt gggccggttg ccattatttc atcctccctg ccatagtctc aacgtgagcc
     7561 gcaaggtgcg ccacgctccg gaactggcct ccggaagccc gccggtacat cacgcggtag
     7621 agactccgct cgattgtgcc gtctgcacgc atcacacgca gagtgcgacg ctgcgcgcga
     7681 atcttcttta tccacgcccg cttgccgggg ccacgcgctc cagcggcgcc cctcctccgt
     7741 cccggtcctt tccggtggcc gtaggaacgc ttcgccatcc gtgcccgggc cctgccgcgg
     7801 ctgttcccct tcacgacgtg agccttgatc acgccttcac cgatcagctc gcgcagatcg
     7861 tttctggaga tagccgcctc gatatcagac tggcgctcgg gatcgaacca gacacggttg
     7921 acaccgcact tgagaacaga ggctgctatt ctcttctggc tggcgagatc actcatcttc
     7981 gttcacctct tcttcgcttc cggcaggtgt ttcacccgca cgagtgagat cttttgcgtt
     8041 cagcaccttg agccccagtt ccagggcccg cgtctgaatc attccccgct ttctgttgcc
     8101 tacggatcca ccgatccgga cggcctgcgt ctcggggttc agtcccacga gttctgtcgg
     8161 ggtaaagacc cgtacatcct catagccgct cgggtgcatg ccgcggattg ccgcggggct
     8221 cccgtatccc ggccggggaa gaacgccctt tgcggcgaac tgccgtctct gcttgttgtg
     8281 tagaccacgc ggacgccgcc agacatctgc cagcctcgat tttcggtgaa gcccccgcct
     8341 cttgaaggcg ggcttgttgt gccgggtgcg gacgcggatc aatcttcttg tttcatccat
     8401 tggtttcacg ccctctcggt gatgtagatg ccgtcctgga acacccgggg gtcacgcttc
     8461 gtgatcctgg tcgcgtgctc gatgtttgcg gctgtgttgc ccaccttctc cttatcgata
     8521 ccggtgagag tcacctcgtc gcttccgatc ttgatggtga cgccctcgat gatatttgcg
     8581 gtccgggact gtttctcgcc gaggaagttg ttgatctcga ggcgcccgcc ccgctgtttt
     8641 aactggatcg ggaagtggtt gtagaccacc ttcatgcgat actcgtagcc ttcggcgacg
     8701 ccccggatca tgttcttcac atgggcctca agcgtgccgc tcatggcgag gaaacgcttc
     8761 ttattggacg ccgtggagac gacgacttcg ccgccttcaa ccttgatatc gatctggggg
     8821 aagcgcatgt cacgggccag cgtgcccttc ggtccggaga ccgtgagcat gctgccttcg
     8881 agcgtgacgt ccacaccggg agggatctcg atctgtcgtg ttattgccat atctctccct
     8941 cctttagtag acgtatccca gcagctgccc gccgacaccc gcgctccggg cctctgcatg
     9001 ggagatgact cccttcgagg tggagatgat caggatgccg aagttctttg cggggaggta
     9061 ctgtgactcc caggattcca tatcagccat ccccaccggg aaccgggggg agatagcgcc
     9121 acacttattg atcgttccgg agagctggat ccggaactgg ccgccgcggc cgtcgtcgat
     9181 gaactcgaag ccgccgatat agccattttc ctgcataacg cggagcatgg cgcctaacaa
     9241 cttgctggcg ggttcaacaa taacctcgtt cttcccagcg tcgccggcat tcttgatcgt
     9301 gctcatcgcg tcagcgattg gattgagtcg tgccatattc tctaacccct agttcatctt
     9361 cttgaagccc attttccggg cccactcacg gaagcactga cggcagaagt agatgccgta
     9421 cttgcgtaca aggccctgct tccggccgca gatgcggcac tcgttggccc cacggccgaa
     9481 cttctttgct ttagcgccca ctgaaggtgc tcctgactct tccgccattc cttacacctc
     9541 cacctggtag tgctcgcgca tgaacgcgat ggcttcctct gtactcactt tctggtccga
     9601 cggcagtttt ctgcgctcgg cgactctgcg tgcgatccgc gcgcctttgt actcgagcac
     9661 cacgttgacg tccatgccgt agatgccgat cgtcgggtca tacgacatgc ccgggaagtc
     9721 ggtgtgctct tcgatgccga aggagacgtt gccggtccgg tcgaactgcg tgacggccag
     9781 ttgccgctcg atgatgttga gcgcggtcgc gacgaacttc tcggcgttcc cccggcgcag
     9841 ggtgaccttg cacccgatgg gagcgccctt cctgataccg aacgccggct gggtccgctt
     9901 tgcgatggtc cggacgggct tctggccggt gatcttcttt acgagatcct cagccttgac
     9961 cagccgctca ccgctctctc cgacgcccat atgcacgacg accttgtcta tgtagatatc
    10021 tctcatcgca ctcatgctga ggcctccagt tggggagcaa tcgcggagcg gccgaccatg
    10081 aagatgtact cgtcaatggt ctcgaactgc tcgccggccg attcgtccat gagaatgacg
    10141 cggttcggga cggaactcgg ggttctgatg acctcgacga tcctgccgac tttcccggag
    10201 tgctttccac cgacaaccat ggccacgttg ccttctgcga aggggaagtg gtcgatgatc
    10261 tggaaccgtc cttcatcgga cttcggggca ccgagggtca ccacgatgga gtctcttgcc
    10321 ttgtaggtgt tgtcggccag gatgtttgca ccgaacgcaa ggttcagctg taccttgccg
    10381 ccccggatcg tggtcttgtt ccggatcttg cagagccggg tcgttgaaga ctccgccggg
    10441 atatcgacgg caaccaggtt gccccgcttg tccagctgga tgcggtagtg cttcccgatc
    10501 ttcgggatcg agacgatatc aaagacacca aggcccatct gggggttcct gcaggcccga
    10561 ccgttgacga tgacatcgcg ctggtgcagg atcttccgga cttcgctcgc gttctgtgca
    10621 agaccgatgt gctcacggag ccagacaccg accggcaggg cgccggcgtt gtggggaccc
    10681 ggagcggtct tcatgacgaa tttttgtact ttcttcggga tgtgccagga ccctggcgct
    10741 accaatcgct tgagatagtt tgacattaag ctccgcctcc gagccgctcc tcacgtagtt
    10801 tgtctttcag gttgagcttc gtgatctgca cgttcgaggg atcgaccggc cggggaacct
    10861 cggttccatc ggacttgctc accatcacgc cgtgcacgac cagccggcac atcctcatat
    10921 ctaccgcatc gacgacgcct tcgttaccgg cgaaatctcc acggagcacc ctgacggtgt
    10981 cgcccttgac cacacgggtt ctccgggcgt tgtgcttccc acggagttcg ggggcaagcg
    11041 atgcggagag gtatcggccc cgggtgtggt tcggtgcgtt gtaacgcgcc ttacgctgtt
    11101 ttctgggttg tttacttgct atgcgtacca ttctttacca caccttacac gattatcgtc
    11161 gccatggagg tgatcttcgg gaaccgctcc gcgatctcgc gggggacggc acctttgatc
    11221 tccgttccct tcggctcgcc gcgctcgttg atgagtacca tggcgttgtc ctcgaacgag
    11281 agacgcatcc cgttcgggcg gcgcatctcc ttcttctgcc ggatgaccac cgctttctcg
    11341 agcttcctcc gcatatcggg ggtgcccttc ttgacgctca cggtcgcgac gtcgccgagg
    11401 cccatgcagg gctgccgacg cctgacaccg tgataacggt cgaccgagat gatctccaca
    11461 agccgtgcgc cggtgttgtc ggaacagacc atctgggagc cggtggcgag ggctcgcgga
    11521 atcttcgcct gcatcgcctt cattccttca tcacctcaac caccacgaag ttctttgtct
    11581 ttgagagcgg cctgcactct gcgatccgga ccacatcgcc aacacccgcg ttgatgcagg
    11641 gcgtgctgtg ggcatggtac cgggatctgc gtttctcgta ccgcttgtat ttcttgacgt
    11701 agtggaggaa ctcacgctcc acgacgacgc taccgttcat acggtcgctc acgactttgc
    11761 cggtaatcac ctggccgcgt accggtaaag tgccgtgaaa cggacaattt gcgtcctcac
    11821 attccgcttc cggaatgggg acgtccaacc caatgtttcg tgccattatt catcctcaat
    11881 tatttgatgc gcatactgat ccgccgttcc ggctgtgtct ccaggctcga tccgtccaca
    11941 tcgacggtcg tgccgtcagg gagccggagg cggaagacgc tgaaccgttt cggtatccgc
    12001 ttttctcccc gcccggtcag gataaccagg gtattacggg tttcatcgac gatgcgaccc
    12061 gataccccgg catgacccgg gttgctcgca tgagcgacca gaacgtcgag gccgatcaac
    12121 tcgtgccgca ggacgttctg gggagagatc atgcgctcct ccgtgcgttt tgttcggttc
    12181 ggatacgcgc aatcgtcctt ttgacctcac ggatccttcc ggggttctcg gtggcaccgc
    12241 cggcgctgac tttcccccgc tcctggatca gttcgaggga gagtttctgc tcctgctcaa
    12301 ggagttcaac gtcggagagc tgcttcactt cctgtgcgcg aaagattgcc atctacctct
    12361 cctccacgaa ctctgcacgg gagttctcct cgagttcttc ctcgaagacc tcgtcgccga
    12421 tctcttcggg ctcggggatc gcgaccggag ccttcttcgg ggcgggttca aggacatcga
    12481 aggtgtcggg cagccgtgca tccggcggaa cgatcttgac ctggacaccg atggtcccga
    12541 gcttcttgat cgcaagtgcg tagcccttct cgacgatcgt ctcgctgggc tcaccgcagt
    12601 gcttgacgta gccttcgatg aacttctggg tccgtgaccg ggcgccggtc agcttcccgg
    12661 caacgatgac ctcgcagccg agcgcaccgg attccataac ccggcggatc gtgctctgtc
    12721 cggcctttct gaaataccag ccgcgctcga gagcgtttgc gagccgctca gccatgatct
    12781 gggcgttgaa gttgggattc tggacctgct ggacctccac ctgcggggac tcgatatcgt
    12841 agatagtggc gaggtcctga gtgagctggc ggacctgctt gccgcctttc ccgatcacga
    12901 taccgggctt ctctgcaaag atggtcacct gggtgccgag cggcgtccgg gtgatgtcca
    12961 tgccgccgta tcctgcccgc ttgagttcct tcgtgaggaa cttctcgacg cggacgttgc
    13021 gcacgccgtc agtgataaat ttcttctcta ttgccattac gccacctcgg tcacgacgat
    13081 ctcgatgttc acggtctgcc tgtgtttcgg ggtagcgcga cccatcgcac gcgggaagaa
    13141 tgccttgtgg ccacggcccg tgttggcggc aacgtggttg attcggagtt tttcggtgtc
    13201 aaggccgatg tannnnncgc ttgtgggcga cattccggtt gaaccgcttg aaggggatgg
    13261 ccttcttgag tgccaccaca tcgacgagat acgcttttgc ctcggtggtg gtcatcttcc
    13321 ggatgaaact ggcaatctcg atcgagtgct tgggagagac gggaagctcg ttcgccttgg
    13381 cgcgagcgac gttctcaccc tcgatctttg ctgaataatc tgttcttgcc atagccatca
    13441 cttcagcggt acgtatttac tcgaccgggt tgcaccgata ccggcgctgc cgtgggatac
    13501 ccttctgcgg gtcagtgcaa actctccaag gtagtggaag accgcctcag gctggatctc
    13561 cactgccagg aactctttgc cgttgtgaat ctctaacgtc ctgccgacca tctcgggcat
    13621 gacgatcatg tcacgcagat gagtgcggat ggttccgtct cctgcccgga gattggcaag
    13681 gagtttctcg tggtcccggg agagaccccg gtcaaacttc ctgcgtaccc gggccggcat
    13741 gatcggcagc agctcagaga gagccatctg ctgtagagcc tcaacggagt ggccgcggta
    13801 ggtaaactct tcacgccgcc gcggcattcg cttctgtgtc ttctttgcca tgcttcaccc
    13861 ctcacttctt ccacttgccg gttctccggg cggcaacatg ccctaccttc ctgccgggag
    13921 acgtaccgtg agatacggtc ttgggccgtc cgcagtgctg gtgtccaccg ccaccgaacg
    13981 ggtggtcgat gacgttcatg cagacaccct tgacgcgagg ccacctctcg gaggaggacc
    14041 tgacgtgcat gtgcttcttt cctgccttgg caaacggttt ttcgccccgt ccgccgccgg
    14101 caacgatacc aacggttgcc atgcaggcac cgttgaacca cttgttcttg ccgctcggca
    14161 tccgaacacc gactctgccg tcatcggcct taccgatgac gagggcctgg acaccgctcg
    14221 agcggacgaa tttgccgccg tcgttgggtc gggcttcgat gttgcagacg tatccaccga
    14281 ccgggatctc ccggagcggc agggtgtttc cgttcttcac cgcgcctccc gggccccagg
    14341 agactgcatc tccgacaccg agtccttcgg tggcgaggac gtagaccttc ccaccattct
    14401 cgatcctggt gagagcgatg ggtgcgtggc gagccgggtc gtgctcgata tcgacgacga
    14461 tcccggtggt cagggcgtca ttcttcccga tgtgccgcag ttcggcctta taccggtgcg
    14521 acggtgcacg gtaggtcgga ccgccttttc cgcggctctg tgctataatg cgatgtgcca
    14581 ttcgtcactc accttacata attcctaacc ggctgaggat ctcttcagcc gccttcgcat
    14641 ccgcaaacgt tacaatggcc ttcttctggc ccttcatggt catcattgtg cgaacttcgg
    14701 tcacttcctg ctcgaagacc gacgccagtt cacgcttgat gtcctgcttg gtggcatccc
    14761 tctgcacgat aaactggagc ttgctctcgc cttcgagcat catcgttgct ttttcagtaa
    14821 cgaacgggtg tttgagtttc atgagaactc ctccagtctc ctgattgcag actcggtcca
    14881 gatcgtcagg cgtcctgcct gcgtgccggg tgccagcagt tcggtgttca actggttgac
    14941 cgtcacggca tcgacaccgg caagattccg ggctgcccgg agcggttcgt tcccggtgac
    15001 gatgagaatg ctcttgcgct gcttgtagcg gcgaccacgc atggtgcccc gccctgcacg
    15061 gactttcttg ctctccttcg cccgctcgac atcggcatag acaccgagtg cagagagcgc
    15121 cgagatgaca tcgctcgtcc ggatgatctc ttcaaaccgg tcctcgaaga ccagtgggag
    15181 atcgccctca aagaggtgcc cgcgttcccg ggcgagatcc tggtatgtgc tggctgcgac
    15241 ggcggaccgg aaggccttct gcttctcttt ccggttgata tccttgacga ggatctttgc
    15301 gaccttcggg ggatgcgctg cacgcccgcc ggttgcctgt ggcactctgg ccaccctgct
    15361 gccgttcttt aagcggggga cctgagcgac accccgaccg ctaccccagg agactgccga
    15421 ggtgcgcatg cctgcatagg ggtttgtccc atgcggctgg aaccgggtgc tctggagcgc
    15481 gaggacggcc cgcttaatca ggtctggcct gtactcttcc gtaaagattt ccgggagatc
    15541 gatctcgtga gcgatctcac cggtcagtgt tcgaacctgt gccttcatcg ctgctcaccc
    15601 ctgctggctc tgcgcgctga cgaaactgat cgccggcgtg tggatggtct gttcaccctg
    15661 ccgtatcgcg gaccgtatcc ggatcagccg cttgttcggt cccgggacgg aacccgtgat
    15721 cagcacgtag ggaccgcgca caaggccgta gtgcaggaac ccgcctgccg gcgttatgtc
    15781 gtcgccgttg gtgccgatct ttatgatccg cttgttgaac tcggtgcgct gctggtagcc
    15841 catctggccc gactgcggga cctgccaccg gacgtggtgt gggtgccatg gaccgagggt
    15901 gccgatgtga cgcttcttgc cgccgcggga gtgtttccgc ttccggacct gaacacccca
    15961 gcgcttgacg gggccctgtg tgcccttacc ggtggtgatg gcggtcacgt cgacgaattc
    16021 gccgacctcg aggttgccgg agatcgtaac ctctttcccg aggattccag cggcgtaggc
    16081 gatctgctcg tcaaggcttc cgccggcgat ccgcatctcc atcaggtcgg ggaccttctt
    16141 cgggacaccg gtgagttcca tgggccgggt gtaagcaagg gcgtagagtt ccgcgaccct
    16201 gccttcgccg attgcactct tgatggtatc aagggcggcg gcggtgtcgt ggttcttcgg
    16261 gacgttgacg cgacgggaca actcctcgtc gagttcggtc gtccaggcct cggtcagcgg
    16321 gtgctttccg taggtgtctt cgccgtatgc gcgcacgcct gccacccgca tgggcgggac
    16381 ctcaaccacg gtcaccggca ccatcacatc cttaccttcg gtggggctgc tcttgtggtc
    16441 atcgaccatg atgacatggg tcatccccac cttgtaacct gcgaatccct gcacagccgg
    16501 ggctcccgta tactccggcc atgagccgta gcggggaacc ggactcttcg cccgctttct
    16561 cgggctgtac gcgagggatc ctctgcgtgg tctgttaatc ttcggcatgt ttcaatcaat
    16621 ccttcgtgca ttgtagcgtg gtgcatcgac tgcaggtata ctgcagagcg atgcgtgcgc
    16681 cggacctcgc gccggatctg ccggcacgat cggccttcta gcgaacccat ctgggcagga
    16741 tatcgaacac ccggccaaaa cgcccggggg tcaggaaaca gttgatggag gcggcaacgt
    16801 gagaggatca gagagacatc cgtctctctg cgtttcgtcg ctctctttgc cgccgtcccg
    16861 gtgtgcaccg ggtgcattcc aatcgtcgct aatagatcct gattctccga cgtttccgga
    16921 aagagtgttc ccggaaccgg gcgcgctgca ctggcgttgg cccagcgtct cgatcagatc
    16981 cagctcctgt catcccgttg tgacgagcac aactccatat cgacatcggc cctccatgtg
    17041 gagggttcga ccttcgcttt gtctggtacc ttccgcaccc tccgccgcgc gtccctgatc
    17101 ggcatatatt gcgaatccag agagatcccg gggatcacag gcatttttgg tgccggctga
    17161 ctcccggacc cgggcggaat cgtgcagagc ggtttcttaa acattactta aaatgagata
    17221 taaaccttgt tatcggattc ccggagaatt tgcgcaaagc agctatttgc tgcaggcacg
    17281 gcatcagttc accggacaga gcggtcaagg ggatgaacga agggctgcgg cgaccccgtc
    17341 gcgggcatgc aaccgggagc gattctcccg cagggctccc gctccggcat gggacctgtg
    17401 cgcgcgctcg gggagccaca atcatccaaa gtacaggtcg ccgtctttgt tgatatagat
    17461 ctcaacgtcc tcgcggatct gcctccccct gaaccgctcg gggttagcgt ccctgagcct
    17521 gttaatgagc gatatcgtgt cgtatttgac ttttaacccc ttcttctcgg cttcagcgta
    17581 gatgatctcg ggggccttga gcaggtcgag gccgctctga agcgtacaga ggtgcacagc
    17641 gtcgagggtg tagaggaact caagcgtctc acgcgcacgc gatatattct cgacagcagc
    17701 cttctcgatg gtacagacgt ttgcctttga ggtatggata atctccgcaa tctgttgctg
    17761 tgtcagccct ttcttccggt agcgcagcac ttctttctgg cgatcggtaa gcagaccttg
    17821 tttcatcaaa catatatgga cggtgtaaat ttaaacactt tcccttaacc cccctcgtct
    17881 ccggacgggg ttttcggggc atttcaaaac gtttatatga gaacttgatg atactatcgt
    17941 gttctcaaat tttgagagag gtgttaaagt ggttgttaaa gtaggaattg cgaaactggg
    18001 aaatatcgcc agtggtgtaa tgggcgaact tcttctcgac gagcgcgctg accgtgagga
    18061 tatcatcacc ttcatggcga cctccggtac gaagctgcag cccgaggata tcgaccgtgt
    18121 ggtcagcaac atgaaggcat ggggccccga cttctgtatc gtcgtctccc ccaacggcgt
    18181 cctccccgga cccgtcaagg cccgtgagga tctcgcagcg gccggaatcc cctgcgtcgt
    18241 catcaccgac gatgtcacca ccaagaagga gcagttcgaa gcgctcaagg cgagcaactt
    18301 cggctacatc atcatgaagg ccgacgccat gatcggcgcc cgccgtgagt tccttgaccc
    18361 catcgagatg gcggactaca acgggaacct cgtcaaggtg ctcgccctca ccggcgcgtt
    18421 ccgcaagatg cagatggaac tcgacagggt gatcgaccag gtgaaggcag gcaagaaggg
    18481 cgcggacctc gcactcccca aggtcgtcat gacctccgac aaggctgtcg aaggcgagtt
    18541 cagcaacccc tacgctctcg cgaaggcccg tgccgcctac gagatcgcac aggcggttgc
    18601 aggcgtcaac gtcaagggct gcttcatgac gaaggagtgg gagaagtaca tcccgatcgt
    18661 cgcgagcgct cacgaaatga tgcgccaggc gatgttcctc tgtgaggagg ctcgcaccct
    18721 cgataagggc atggacgccg ttatccgcaa gccgcacaag aagaccggtg agatcgtctc
    18781 caagacgacg ctcatcagca agcccgagta aatccaacac cacttctttt tttcgcatct
    18841 tgccagccgc aagttcgccg acgctgcagc agcagagagg aggctgcatc tacgagccct
    18901 tgccgggtta aaggggccgg ctcaccagaa tagaataccg ctggcaggcc acgcctcctc
    18961 acggggagaa gaccatcatc cgctcttccg tcatctcctc gatcgcatac ctcggccctt
    19021 cgcgcccgat gcccgaccct ctgaccccgc cgtacggggc gtggtccatc cggaacccgg
    19081 agatgtcgtt gaccaccagg ccgccgaccc ggaggtcggc gaacgcctgc gcgatcctcc
    19141 gggcatcgtg ggtgaagagg cctgcctgca gcccgtagtc ggagtcgttg gcgagtgcta
    19201 tcgcctcctc gaaggtatcg tagggggtga ccgtcaccac cggggcgaag acctcggtcc
    19261 ggttcacctg catctccgcc gtcgtgtcga caaggaccgt cggcataatg agcggaccgt
    19321 cacgggttcc cccggtgagc gctctggcac ccccctccac cgcttcctgc accttcgcaa
    19381 acgctgctag tgcggcaacg tccgtgatca tcggccccac gtcggtgccg ggctcgcgcg
    19441 ggtcgccggt ggcgagggca cggacccggt cgatgagcag cgcgagtgct tcctcaaaga
    19501 tgggccgatg gagatagacc cgctgcaccg agatgcaggt ctgccctgcg ttcgagaatc
    19561 caccggcaac gatacggccg accgcgaagg ggatatcggc atcagagtgg acgatcacgg
    19621 ccgcgttgcc gccgagttcc agtccgaccc tcttacgacc ggcaatctcc cggaggtgcc
    19681 acccgacagc cgggcttccg gtgaaactca cgcaggccac ccggtcgtcc cgcaccatcc
    19741 gttcagcaag gtcagcggag catgggacga cgctgatcgc ctgtgggggg aacccggcgt
    19801 caagcaccat ctccccgagc atcagcgcgg agatcggggt ggccgacgcg ggtttgagga
    19861 tgacggagtc tccggcggcg atggccggcc cgagtttatg gcaggcaaga ttgagcggaa
    19921 agttgaacgg cgtgatcgcg agcaccggac cgagcgggaa ccggcggagg cagccgaccc
    19981 gcccttcgcc ggccgtgttc cagtcgagag ggaggatcgt gccgtcgatc ctgcgcgctt
    20041 cctcggcgga tacctggatc gtctcccggg cgcgcaaaac ctcattttcg gcgagaaggc
    20101 gggtctttcc cgcttcgagg gcgatcgtcc cgataagttc ttcagaacgt tcccggatga
    20161 ggccggcaag ggcgtagagg atctctgaga ggcggtgggc cggaaggcgc cgggtgaggg
    20221 agaaaccgct ctctgcagaa tggagggcgt cctcaatatc cccgcttccg gcaaggcaga
    20281 cccggccgac cgtctccccg gtgtagggga accggacatc caggatctca ttgcttctcc
    20341 gccagtctcc ccctatcagg aatccgcgcg cgctcgtcat ggtgaggagt taacgccggc
    20401 acgcaatcaa cctttctccc gaaagagggc tcccagggac tcggggagcg tcaggatgac
    20461 cggctccagg tgcagccgct ccatgaacgc cgagtcgcac atggagaggc ggttcgggtt
    20521 caaccgtgcg atgagggagc agaagacccg gtggccccgc cccgccgttt tgggggcgtc
    20581 aaagaagatc gaggcgttga aggcgtgcgt gccgaggctc cggtagaact cgaggatccg
    20641 gaggatcccg tcggccagcg gctcgacata cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
    20701 nnnnnnnnnn ngcgaggtag cggcggccgg cggatatgta gaggtccgca aggcgggtgg
    20761 gctccggatc ggcgatgccc tgcaggtgcg ggtggatcat gctcgcaccg gccgacggga
    20821 ggtagttcca gttgatgctc gcgtagccgg aagaccgggc cagggcttct gccgtgccgg
    20881 atatggcgtc ggcaaggcat ctcctgtcga accggccggc cgtgtgatcg ggcgtgatca
    20941 ccgtgaccac gtgacatgcg gcgaacggat agaggttggg gaacgtcacg ctctccccgc
    21001 accggagacg gctgccgtcc tgaaacgtcg gggtggaggt ttctatggca tcggggcaga
    21061 acggacagcc gtcacgggat gagggcaggg caggggcggc atctatctgc cgatcgaccc
    21121 ggacgggact gatccggcac cggatgccgg tgagggactc ccggcggtac tggagaatgc
    21181 cccgacctgt ccggatctca tgggtggtga acatgaccct ttgtactccg gttagcgcgc
    21241 cgaccgtata aaaccctttc aaaatcgccg gggcgagaat caaaggtttc ggaggggtag
    21301 gtgaagcgtc caggagagtc ggggatgcct gcggaaccgg tcagcatgag ctcttgagcg
    21361 gagccgttcg ggacaaaaaa aggtgatgag tgttctcctg gccttacacg acgtacttgc
    21421 cgagcaggcg gatggagttg gtcatgtcgg ggccgagcgg cgagccgaag atgatctggg
    21481 taactccagc cttcgtgagg tcttcgcact tctgcttgac catgtcgggg gttccggcaa
    21541 tggtgaaggc gtcgatctcc gcgtcgccga caagtccgcc gacggtcttg aagtcgaagt
    21601 ggtcgagcgc ttccttgatc ttcgcgacgt tggcgaggtc gaggttgtgg cgctgaagga
    21661 gcgcgggcgg ggagcctgcg gcgatgaacg cggccacaat ctttgcggcg ttccgggcct
    21721 tcttctcgct catgtcgatg gacatggcgg tgtaggcacc gacgtcgaag gtcttcttgc
    21781 cggccttctc catggctccc ttgatgatcg ggatggcgat ctcgaagtcc ttggggttgg
    21841 acgcgttgat cagggcgccg tcaccgatct cgccggcgag ctcgagcacc ttgggtccct
    21901 gggcaccgat gtataccggg atgcccttct tgccgggcag gttgacaccg gtcagcttgg
    21961 cgccgttgta gtcgaagaat tccatgttgc cggacttctt gacctcttca ccagcgagga
    22021 gtttccggat ctgctcgacg ccnnccgggt ccgataccga ggacggcacg tccgtcggag
    22081 atctcgttta aggtagccat aaaggaggca atggctgccg gggtgtcggt gaacgtgttc
    22141 atgatgccgg gtcccatctt gatcgagtcg gtgtttgccg cgatcatggc gagggtcggg
    22201 taggcatgac ggttgttgta gtggttggtg atccaggcgt agtcgatatc cttagactct
    22261 gcgagcttgg tgaatttcac cacctgcttg acgttgattg ctccgggcac aaattcaatt
    22321 ccatatgtag tcaaggctat tcacctcatt gagtagttac gtgcagagag ttatatatat
    22381 tatcattttg tgctcaccaa gcgacacgta ccgcacaggt agtgcaaaac ggagatgtgt
    22441 aacgggttcc ccaggacatc cggtgaatct tctcccttat cctccggagg gctccagcac
    22501 ggggaagcca aaccggaata tttaagtgcg ggtattgtgt gaacggacgc gctcccgccc
    22561 ccgggggacg tacagaaatg tatatgttcg tccgcccaca tactcatcag tagttctggg
    22621 tgatatgcat ggttttttcg cggcggacgt cactttgggc atgcgaaatt accaggcagg
    22681 gggtatgatg ttatgcgagt gctgacgatc gggttgggtg gcgccgggtc acgggttgtg
    22741 gatagcctct acgaccacga ccggcgaagc aaaatctgct gcatgaatgc agtagcgatc
    22801 gacatcgacc cgaactccct ggtgcaactc cgctatctcc cggaccccgc gagggtcttc
    22861 tttccgcgga cagatctcac ggaccccggc gagatcacgg acgtgatcga tatcgaggag
    22921 gtgatgaccc ggctccaggg catggatacg atggagatcg acgctatctt cctctgttgc
    22981 gggctcgggg gcagcgtcat cgatatcgca ccgctcatca tcgatgaact ccgaaagtcc
    23041 tacatggagc cgatcttcgc tcttgccata ctgccgtgtc tggaggaggg gaaacgagtc
    23101 tcggccaagg ccgccgacga cctcgatgcg ctccagagca tcgtcgatgc cgtcatcctc
    23161 ttcgacaacg agacatggtc gcgtaagatc cgggcagccg cagctgcggc cgagacagag
    23221 aataccggtg tcatgggcca gctgcgccgg agcccccggg catctgaccc gagaactcac
    23281 tataatatgc tcaacgagcg gattgcacga cagatcggcc tccttctgcg cgcaggtgag
    23341 ttcaatgaat cagggctcga ttacgctgag gtcgtcctgg atgccgggga ggtcctcaac
    23401 accctgaagg ggaacagttt cgtcgcggtc ggctacgcgg ccgaacgcct gccgaccggg
    23461 tggctgaact tcctccagcg gcggcggtca ctgaagtact tcatcgaggg ttcgcaggag
    23521 aaggcctccc ggattgtttc actcgccaaa aaggcggtct atgaagaggt atcggtnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagga acacatcatt
    23641 cgtcgggatc atcatcgtgc tctcggggct ctccaacatc ccccgcgtcg aggatatcca
    23701 ggatatccgg gacgaatacc ggcttgaatg tgaggaagaa caactgaagg ccgaagagga
    23761 ggagcggctg cgcaatgagg agggagcccg ggcactggag cccgagtccg gcgccccgga
    23821 gccggacgat atcccgggtt ttgcctcccc cctcgagtcg gcatacctgg aggagatcgg
    23881 atacatgacg gaaccatcta caccagaaag agatgagatg atcatgctcc cgggcagcgg
    23941 cgagggcggc ccaaaacgca gggacgacac cattgatata cttccgagag ccgggaagga
    24001 acaggannnn nnnnnnnnnn nnnnnncctg aaggggatca gcatcgagaa gaccgcccca
    24061 aaagacgatg cgataacata ctccgcagcc ttaaaacccg tgcagcgccc gaaggaaggg
    24121 gttttcaccg gcgatacggt caggctggac cgcgggatgc agcggccgag cgacggcgtc
    24181 ctcgatgaac ccggcctcca catgcgtggg gcggcccccg caccgaagga tgccctgctg
    24241 gaccagaccg gccgggggtt tggacgcccg gctccccgac caaaggaggt tggcccctcg
    24301 cgcgggaggc ttgcggtgat cgatgcggcc gcccggcaaa agagcagcga tgaggggaag
    24361 aaatcggacg ccgacacgga cgatggcggg atcacctgga tccagtagcg tccgctattc
    24421 gctcaaacgg tatggtcccg gcaccaacct cggttctgac ggcatagccc ttctcaatgg
    24481 cgttccccac catgttcatg ccggaaaacg cgacgacgga aagccgatag gggtgtttgg
    24541 cgaggttgaa ctccccgatg caccctccgg cggggaaggt gaatccacac cccgtcatca
    24601 gcctggcggc ctcctcaacc ctctcctgcg cggccgcagg caccgcacgg atgtttgcaa
    24661 gtcctacgcc tgcccgtgtc gtcaccagcc ggtttattga ggtgagtccg gcggatatga
    24721 agaggtggag cgggtcgacc gatgttcccc ggtagccgat gagatcgagg aacccggcgg
    24781 agccattctc atcaacggca atcctgccgg catactcgag cctcgccggg atacccatct
    24841 tctggaggac gccgtctaac gtgacgctgc aggccgtcat gatcccggcc tggccaccgg
    24901 gtatgcgggg gtccgaatcg acgatccggt aggagttcag gagcccgtag cctgcggttg
    24961 cgacctcgtc gaaggctgcc gtgacgccgg gaaggtcctc ctcccgcacg aaggagaggt
    25021 tgtaggcgac gctgcccgtg gcggtcaccg ggttgaaggt gctccggaag gagagttgct
    25081 cgagttttga gatgatgaag ccgatccgct cgtcgacgag cgcgctctcg ctctcgctga
    25141 tccccgccag ggtgaggatg cggccccggt tcccgacctt ctccgtaaac cccatctcat
    25201 cgaggtactg gaggtagtac tggacggcgc ggtcgctgag gatgaaaccc cgctcggcca
    25261 tcttttcgct caaccttttt gcacccaggg gttcgtgcga ctccgcgagg atccgcagga
    25321 tctcaaggta cttccgttcc gaacgtatcg acgtgctcat cgtgtatgca ccgcgccttc
    25381 gctatcctgg tacctgccct ggtagagccg gtcgccgctc agcacttcct gcaggatcat
    25441 ctgcacgacc cggtcgcctt ccagaaggcg gacctcctct gcgccggcgt tcgtcaggca
    25501 gagcgtcagc tgcccgcgga accccgggtc tacgaacccg ccgccgagga ggacgccgcg
    25561 gcgggcgagc gaggagcggc atcggagggt tgccgcgagg tcccccggga gttccacccg
    25621 ctcgaccgag gggacgaggg tgcatgcgcc gcacgggagc acggtctctc cggccgcccg
    25681 gaggtcgtaa gatgcgggct gctgggatgc gctgtggtat ggctcgatga caaggtcgcc
    25741 atcccggagt cggcgaacga tttcgctgga cgagaggatc attgtataga gtactggcac
    25801 aaaaacgctt aataacttat gatagaaatc agagagggga tccatggggt agtggacatc
    25861 cttttgggct tcgaacccaa ggacgcgggt tcaattcccg ctgggtccgt catttttccc
    25921 gggtgcgcga agtgcgcacc cagtaattgt cacgcgagtc ggatatacac cgtatggtgt
    25981 tctccgaccc ggatcggggg gaagagtgcc ccggcatcgg gtgcgtcgtc ggcagaggca
    26041 cgtatccgga ccaggtattc acccggagga aggtccccta catcgaggga ggaggcttcg
    26101 ctggtcacgc cactgtttgc ggtcggggtg tcaaaccgca cccaggagag gccgccgtcg
    26161 tttgagtact ccacgctctc ggtcacggtt cccgacccgg tgtatgcgag gtcccaggca
    26221 acggtgagga gttccagcac cggctctgcg caggtgtagc gcgggtttga gacgccaagc
    26281 gtcgcggagc ccaggcccgt gttggtgagg tcaggcacgg ccgtgatgaa ggtgtcgggg
    26341 aggggcagcg tatcggtgcc gtcgttgaac gtgaccgccg agttcggtcc gaagacgttg
    26401 atgttgccgt cagtgaggac tttgaggcgg aacgttgcct cccaggtctg gtcgaggtgg
    26461 acggtgccga tattgaattg cagggcgtgg tcgtcgttcc agtcgtcggt ctggttgata
    26521 gtaataaagg gagtgatcgc atgatgtccc gtatcgttgt cgacccagct ctcgatggtg
    26581 gtcgagatac cgtcttcatg gacgtaggtg aagaccgtct ccccctcccg gtcttcgtcg
    26641 ttgacctgta tcgtgctgaa gtcgagagtc atggcagtgt tgacgccggc ctcggtcttg
    26701 agttcgccgg caatctcgga gtagatagca gcgaggtcgg caccggtggc ggcatggtag
    26761 tgtttgccgc cggtcgcccc tgcaagggtg tccatcgtct tccatgtggg gctttcctca
    26821 ctaataccgt tcgagaacga tatcatgtac aaccggatgt tgttgttctc cgcgtagacg
    26881 gacatattct ggttcgtgtg caggccgcca tctgcctctt ttgaaccccc gagaccctcg
    26941 aagaaggtat acctgtccgt ccgggtatcg gtccaatcat acttatcgcg cccttcaccc
    27001 cgggcaagcg ggtccccata gtagttgtac tcaccgtcgg agagcagaat gacggcctgg
    27061 acggcatcgg gatcgggatt tgagtgcgcg atcatctcct ggatcgaggt atagagcgct
    27121 tttcgcgtgg cggtccatcc tgaaggggtg agcgcattaa tggtagtatt tacactcgaa
    27181 taatcatgcg tcagatgcag ggcgcacgag gcgtcattgc catactgaac caggccgatt
    27241 ctatcccggg cgggactcat ctctgcaacg aaggtctttg cagcagtttt cgcactgctg
    27301 atccgggatg gattatcatc cgccatactc cccgaccggt ccatcaccaa tatcacatcg
    27361 atcgggtccg gctggagcgc ccagccgtcg ccccggaggc ggacggtgac atcgaccgta
    27421 tcggtcacgt tgaccgtccc ggggacggcc tccgcctcca cggagaggta ggggtagttc
    27481 tcccaggtca gggggatcgt ccgggtggtg ccgttccacg tggcgacgac gtcgcacccg
    27541 ccccgggcgc tgccgctcca ctccggatcc tcccgatcgg tcgtgaacgc gcccgggtag
    27601 aatcggacgg tggcgtggcc gtcgccatcg gtctctgcag aggtctcggc gaggtacggg
    27661 tcactgacct gcacgaaccc gccggtatca gtgttccgga tcgagaaggt cacgtcctct
    27721 cccccgaccg ggtttccttt ctcgtcgatg accttcgcac ggagggttga gacggacgat
    27781 ggggtcaact acccccggtt aaaaccgtgg gcttgcagct agaatcatcg gag
//
LOCUS       DAJB01000175            3060 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_100058, whole genome shotgun sequence.
ACCESSION   DAJB01000175 DAJB01000000
VERSION     DAJB01000175.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_100058"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          519 a          988 c          974 g          579 t
ORIGIN      
        1 tgcagtagtc gaggtcgata ttgaggtcct cgtcgatgac cccgtccggg cagtactggg
       61 cgcagagccc gcaggcgttg cacgcctcct tgtccaccac gggccggaac gtccgccagg
      121 aacccgtttt ccccgaagag gcctccgtcg gcctgctgag cgcaagtctc tctctcatac
      181 cttcaactcc tcatacgccg cttttgcagc cttcacattc cgttcgtcgg caaacatctc
      241 ccggatcgcc tgctctgccg aggggagggt gacgatatcc atcttcgcga gcgccccgag
      301 caccggggtg ttgaggatgg ggctcccggc aaccaccagg ttctccgcga gcgcgattcc
      361 ggtcaggtcg acgtgccggc aggtgccgtt gcacccctcg atcggatggg cactgttcag
      421 gagaactgtt ccgccagatt ttaagccctg caggacgtcc accacgtcca tgatgctggt
      481 atcgagcacg attacgagat ccggctcatg gatctggctg tagatcttta tcggccgatc
      541 atcgatccga acgaacgaga cgaccggtgc tccgcgccgc tccgccccgt agaacgggca
      601 ggcggttgcg tattttccgt ccctgaatgc cgcgagggcg aggagcttcg cggccgtgac
      661 gccgccctgc ccgcccctgg agtgaatcct gatctcatac atggtcgctc accccaaacc
      721 aggtctcctc accgggatgc cggttccgaa caaagtccgc gatatcgttg taggtgacct
      781 cctggccgcc gaggccggcg atcacgctgt agggcacgat gcctgtcttc gacctgatag
      841 aatgcgccac cacgcctcca aagccgaagg agtagtcgcg gtcgatcacc acgacctccc
      901 gcccggcaag gtcgaggtca gggaaggggc ggaaccagcg caggcgcatg gacccggcct
      961 ttacaccctc gtcgcggaga cggtcgaccg ccacctccgc ctctttcccg agcgttccca
     1021 tggcaacgac gatgacatcg gcatcttcgc accggtagtc ctcggtgggg ccgtaggagc
     1081 ggccgaaccg ctctgcaaac tcgcgctcga cgtccgcgat cacgccctta gcgtcgcgca
     1141 tcgaccgctc gatatcccag cggaaccgga agtagtcggc cggtccggtc atggggccgt
     1201 agccgcaggg gttctctgta tcgatcgcgt gcgggagatg ataggcaggc aggaagtctc
     1261 ctatctcaac cgtttcgagc gactgcatga tgtggctcag cgagaacccg tccaggttga
     1321 ccatcaccgg gaggaggacc ctctcgtcct cggcgatctt aaacgccatc agggtggcgt
     1381 cgtaggcctc ctggaccgtg cccacgaaga cctgcagcca gcccgtatcg cgctgggaga
     1441 aggcatcggt gtgctccgcc caggtgttcc acccgggacc gagggcacgg ttgacgtttg
     1501 ccatgacgat cggcaggcgc gcgcccgccg cccagtggac catctcgtgc atgtaaagga
     1561 gaccgtgcga actcgttgcc gtgaaggtgc ggacgccccc ggcgctcgcc ccgatacagg
     1621 cggccatcgc ggagtgctcg ctctccaccg ggatgtaccg gctttcgagc tcgccggtgg
     1681 tgacgtactc ggcgagctgc tcgacgatct cggtctgggg ggtgatcggg tatgcggcga
     1741 cgacacccgg ttttgcggcc ttcaccgcgg ccgccaccgc cttgttgccg gtcgcgatcg
     1801 tcagcatacg ccggcctcct cgcgggaaag gcgctgaatg ttccggtcca cctgctgccg
     1861 gatctcgctc atgacctcag gactcaggtt cttgaaccgg ccctggccgt tgatgtagtc
     1921 ctcgatcggc gccggcttcc tcatcgcggc cttcgacggg ccgctgatcg cgagtttccc
     1981 gaactcgcgc tcgtagagaa tccactgggc ggtcttcacc gccagtttcc ccatctcgac
     2041 ggtcttctcc gtggggtagc gccatccggg cgggcacggc gccaggatat ggatgaactt
     2101 gggcccctcg atcgagagcg ccttcttcac tttcttgtag aggtcgagtg ggtaggcgct
     2161 gcaggcggtc gccatgtagg gggggttatg cgcctcgacg atcctgtcga ggtccttctt
     2221 ggccgtgggc ttgccgccgg gggtcgtggt ggtgcgtgct ccgagagggg tcgagcccga
     2281 ccgctgcatg ccggtgttgc cgtacgcctc gttgtcgtag cagatgtaga ggaaattggt
     2341 gccccgctca aaagcgccgg agagcgactg aatcccgata tcgaccgtcc cgccgtcacc
     2401 tgcgtagacg atgacgttgg tctctttccc gagcgcgcta agagcgttgc tcaggccgga
     2461 agcgcaggcg gcggctgcgg caaacgctat attatagacc ggcacgttca tcgccgtgtt
     2521 ggggtagatc ccctggatga cgctggtgca gcaggcgggc acgaccagaa cggtatcggg
     2581 gccggccgct ttgagcacgt agcgcagaca cagggaagag ctgcaccccg cacaggcgga
     2641 cgtgcactta tacagatact cctccttcgg aatcgcggac aaattgttta cactcctctc
     2701 atctatattt gtcaccaatt ataatgagag ttttgcggcc ccgcccccgg gtatccggcg
     2761 gccttccgga ggaatggtgg gtgaggatgc gccggaatac gggatgcgtt gaaaaacggg
     2821 ccgggctgga gcccggttaa atcggacccg gcgcggaccg acttgccggg ggttccccgg
     2881 acccttcacc cccgttcagt tcccggcacc actccccggg gtgcgcctct gcggcgccga
     2941 acctgggcga agcgaccggc ggatcaggcc ggcgaagcac ttcaggcaga agggcgtgaa
     3001 gaaccagacg cccatctcca ggaggggaag gggtccgttg tggcgtatcc ggtaccggta
//
LOCUS       DAJB01000176           17289 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_39724, whole genome shotgun sequence.
ACCESSION   DAJB01000176 DAJB01000000
VERSION     DAJB01000176.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17289)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17289)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17289
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_39724"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    11437..11514
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3233 a         5574 c         5439 g         2963 t
ORIGIN      
        1 gccgcggccg ccggcatgga tctgggcctt caccacccag accttgccgc ccaaggtttc
       61 tgctgccttg acggcctcat cgacggaaaa acagggaatg ccgcgcgggg tcgccacgcc
      121 gaatttcctc aggatttcct tgccctgata ctcgtggatc ttcatggatc ttccttgccg
      181 aggggattgg tcaaacccgc atggcctgtg ccgcggggca gttgaataat atcacgttat
      241 gggtgctgca tttttactga tgaagccgcc agaccctaaa cctcgatcac ccagtcgatc
      301 gacgatggcg gcaggtagtg ctgctgcacc tgcgagaaat agaagcggtt ggcgacgaag
      361 gccgcatcga tcatgccgca actggccgcc ggattggcgc cgctggcatg gaagtcggcg
      421 aagccggccg gctggttgat caggaccgcg ccggtcaggt tgaaggaaag cgccacgccg
      481 gcacgcagcg cgacgtcttc ggcctgctgg cgcacgaagg ggcgcgtcgc atgcacgaga
      541 aaattcagcg cccccttctc gcgcatcgtc gtttcggcga gcgcgagact ctcggcggtg
      601 ttcgcggtcg tcaccaccgc ggcgatcggc ccgaaccgct cctccatgta gagggcgcgc
      661 tgcctggcat ccgcgcgcag cagcagcggc gtgtggatgc gcgccgccgg ccactgcgga
      721 tggctgagcg gcacggacgg gcgcagcacc tcggcctccg cttccgccag ttcgccggtt
      781 gccgcgatgc gcgcgacggt ggccggcgac tggattgcgc cgagcagctc gaccgcccgc
      841 accgggtcct cgacgaactt gcccagcgcg tagccgaggt cgcggccgaa ctgctccgcg
      901 ccgacctgtc cttccggcgt ctggacgcct tccgcggcga cgaagatcac gcgcggggtg
      961 gtgcgcagct ggccggcatc gagacagagc gagaacacca ggttcttcag cagcgcctgg
     1021 tagtcgtagg tcgagtcgac cagcacgcag ttgacgccgc cggtctcgac gaacagcctg
     1081 gcttggcgag cgttttcctt cagccagtcg ccgaaatcgg gagcgccggc atagtcgatg
     1141 aggcggatct cgggatgctg cgcgatatcg cgcgcgaccg gcccgtccgg gttgctcgcc
     1201 agcaggctca cgaggttggg gtcgaagccg ctttccttga gcacctggcg ggcgacggcg
     1261 accgagatgg ccaggggcag cacggcggtc ggatgcggct tgacgatcac cgggttgccg
     1321 gtcgccaggc tggcgaagag ggccggatag gcgttccagg tcggctgcgt ggcactgccg
     1381 atgaccagcg agattcccag cggcgcgacc atgaagcgct tttcgagccg ctggctgacg
     1441 ttgcgctgcc actccgcctg ctgcgtgatc tgcgccatct cgcggtgcgc ccaggccagc
     1501 gcttcgagcc cgcgtgcctg cgcctgcggg ccgccgctga gaaaggcgag ggcgaggctc
     1561 tggccggtgg tgtgcatcaa ggcatgggcc agctcggcgc tgcgcgtgtt cagccgcgcc
     1621 aggatctcga ggcagattcc cgtgcggctc tcgatgccgg ccctgaccca gggcaacagc
     1681 gccgccttgg ccgccgcgat cagcccgtcg gagctgcagg acgggtagga aatcgccaag
     1741 ggcaaaccgt agggggagat ctcgtccccg cagcgtccct gcacgtcggg ctggtcgaga
     1801 tagaactgcg cgccgcgata ggcctcgaac gcggccttgc catcgtccat cgccgtctcg
     1861 ccataggcgc gcggattcgc cgcataaggc agccagtgcg tgcggcgcgt gatcgcggcc
     1921 tgcgcctgct cgagcaaggc gcgatgtttg tcgaacagcg gatggaacat ggcggtctcc
     1981 caataggcgc cgtgataaag ttcgccccgt cattgtattg tcgcaacggg ctttacgctg
     2041 caccgggagg aacgcaagaa tgaaatcgga tcgggcgctc gacgccatcg aaaccgccag
     2101 ccgcgacgag atcgtcgccc tgcagaccga gcgcctgcgc tggagcctcc ggcatgccta
     2161 cgacaacgtc gcccacctgc gcgccaagtt cgacgccgcc ggcgtcaagc cggaggattt
     2221 cagggaattg cgcgatctcg ccaagttccc cttcacgacc aagcaggacc tgcgcgacac
     2281 ctatccgttc ggcatgttcg ccgtgccgga aaacaaggtg gtgcgcgtcc atgcctcgtc
     2341 cggcacgacc ggcaagccca cggtggtcgg ctacacgcag aaggacatcg acacctgggc
     2401 cggcctgatg gcgcgctcga tttacgcggc cggcggccgc cccggcgaca aggtgcatgt
     2461 ctcctacggc tatggcctgt tcaccggcgg cctcggcgcc cactacggcg ccgagcgtgt
     2521 cggcgcaacc gtccttccca caagcgtcgg gaacacggag cgacaggtcg aactgatgca
     2581 ggacctccac gtcaccgcca tcgcctgcac cccctcctac ctcctccaca tgggcgaggt
     2641 tgcggagaag atgggcgtct cgatcaagaa cgataccgac cttcgcatcg gcttcctcgg
     2701 cgccgaaccc tggtcggagc agatgcgggg ccggatagag gactggctcg gcattcgcgc
     2761 ctacgacatc tacggcacaa gcgaactctc cggcccgatg ttcaccgaat gcgccgagca
     2821 gcaggggatt cacatctggg gggaccttgc gctcgtggag atcgtggacc ccgcaaccgg
     2881 cgaagtgctc gcgcccggcg aacagggcga gctgacgatc accatgctgc agaaggaggc
     2941 tctccccatg gtccggtacc gtatcgggga cctcaccgcc atcgaagaga gtccctgcgc
     3001 ctgcggccgg acccacccgc gcatcggccg gatccgcgga agggtggacg acatgctgat
     3061 catccggggc atcaacgtct tcccgtcgca ggtggagcat gcgctgcttg agatcccaca
     3121 ggtcggcagg cacttccaga tcgtcgtcga ccggaagggc gcgctcgatt ccatgctcgt
     3181 gcgggtagag gtgagcgaag aggcgttctc cgacaagatc accgatctta tggtgatcaa
     3241 gagaaaggtg gagcatcgcc tccggggttc cctgaacgtg ggcgtggatg tggaactggt
     3301 cgaaccggga actcttccgc ggtttgaagg caagtcgaag aaagttgttg acaggaggtc
     3361 actataatgg agaaatcgta tatcgtcaaa cagatctcaa tcttttcgga gaaccgcccg
     3421 gggcgcctgg cggcgatcgc cggtgcgttg cgggatgccg ggatcaacat ctttgcgttc
     3481 agcatcgccg aggcggacgg gttcggggtt gtccgtgcgc tcgtcgaccg gccggaggac
     3541 gcccaccgga cgctgaccgg tctcgggttc cgcgtctcgt tcaccgatgt catcggcgta
     3601 aagatgcgcg acgaacccgg cggcctctcg gatattgcag ccctcctcgg ggatgagggg
     3661 atcaacatcg agtacgccta cgcctactca ggcagggacg gagcggtcct gatcctgcgc
     3721 gtggatcagg ttgaggacgc catccggcag atccttgacc gcggcggaga actcctcaaa
     3781 gcgtctctct cgcggtaatc cccgggctca gaccgggctc cggggcactc ccgggccctc
     3841 gggctcttct gcctcatctt tgaggaacat ctgaaccctt cccgcgatgc gccggatcgg
     3901 gaggacgaac gcgaggccca tccccggccg gtccagttcg gcggcgacga cgagggtgat
     3961 ggggagagga ccgcacagag aacctcgaag actgttcacc tgagaccctg caccaggata
     4021 catccctctc acgatttcgg gtgtcaggta ccctgccgca agcgcagata agagcggtgc
     4081 tcgacgccga tcagtgccgc ttctcccatt tcaggagttc agagacctcc gaaagcgtct
     4141 ttccgcgccg gatctcctcg cggatcctct cctcggtctt tttgacttcc atcgcccggc
     4201 gtgcgacctc gtaggcccgc tcccggggga ccaccacgac accgctctcg tcggcgacga
     4261 tccagtcgcc cggccgtacc tcctgcccgc aacaggcgat ctcggtgttg atctccccaa
     4321 gacccttcgg gtccccggcg ttcgggacgg tcgccgtggc gaagagcgga aatcccattt
     4381 ccctgatatc gtcgatgtcc cgggccgccc cgtcgatcac gacgcccgct atcccccggt
     4441 tccgggcgga atgcgtggcg agttctcccc agggcgcgat ctccctcctt ccatcgttgc
     4501 tgatgacgag gacatctccc ggcccggcaa cgtcgatcgc ctcgacgggc tttgcccagt
     4561 ctccagcgac ggtacgaacc gtcaccgccg ggccgaccgc ctttaccctg ccacagatgg
     4621 aaacgatgcc cgccatcgcc cccttccggt gcatggcgtc ggtgacgttc ggtgcggaga
     4681 cggcttcgaa aaggcggcgg atgacggcat cggggctctc ctcttcccgt ggcctgatct
     4741 cgggccggtc aatagcattc cggacccgcc gggccgcccc ggtcacgtcg gcagccttga
     4801 tgatgttgcc tcccacaatg acgatcgaag ccccggcggc aaccgcctct gatgcagtct
     4861 ctgcatcgag cccccctgcg acggcgacgg gcgtgcccac ttccccggca aggcggcgaa
     4921 ggaggtcgag cgacgatttg ccgatcatct gctggtcgat gcctacgtgg gcgttgatgt
     4981 aatcgacgcc gagcgccgca agttctcggg aacgggagac ggggtcggcg acgttgatga
     5041 ggtcggccat gagccggacg ccgtacttgc gggcggaacg gaccgcctcg gcgatcacgg
     5101 tgtcgtcggc atcggcgagg atgcagacga tgccggcacc ggacttcgcc gccatctcca
     5161 cctcgacagc cccggtgtcg gcgatcttca tgtccgccac gatctcgtgc ccggggaaac
     5221 gttcccggag cgtccgcaca gcctgcatcc cctcgctctt gatcagggga gtcccgacct
     5281 caatccagtc cgcaccacca cggaccgcct catctgcgat ctctaccgca cgatcgagct
     5341 cgaggagatc gagcgccacc tggagagtag gcgttgccat acagtacagt atggcgaggg
     5401 ttctatttat ctggtgccgg accggggaaa cctatatgct atgggatgct tgaggtagcc
     5461 ggcgcccacg cctgccctat catgccccga agacggagcg atagggcgac tgtatcggcg
     5521 ggatgtgatt gaaggatggt cgaaattggc tataaactgg catgcgaaga gcacggcccc
     5581 ctggatcttg tctgcaacgc ccggcaggcg gaggatgccg gctttgcatt tgccatgata
     5641 tcggaccact accacccctg gacgagccgg cagggagaaa gcccgttcgt ctggggggtc
     5701 attgggggca tctcccaggt gacggcggat ctgcagctgg tgacgggggt cacctgcccg
     5761 acgatacgga tacaccccgg cgtcatcgct caggctgccg caaccgccgc cgcgatgatg
     5821 ccggagcggt ttatcctggg cctcgggtcg ggcgaaatcc tcaacgagca cgtcttcggc
     5881 gacccctggc cccctgcgcc ggtccggata cagatgctcg aggaggcggt ggaggtgatc
     5941 aggatgctct ggaagggggg catgcaggac cattacggct ccttctacac cctggagaac
     6001 gctcaggtct tcacgctccc ggaaaaactc cccccgatct atatcgcgtc agaaggcccg
     6061 atcagcgctt ccatggccgc ccgggtcggt gacgggttca tcaacccggg cagcaacgcg
     6121 gaggagaacc tcatcatctt ccgggactcc gggggcggcg ataaaccggc atacgttgag
     6181 acttcggtct gctgggccga aaccgaggag gaggggcaga cgaccgccta cgagcagtgg
     6241 cccatcgcag caaacaccgg ggaactcaac cgggtccttc ccacgccgac acactacgag
     6301 caggtggcga agatggtgac gccggaggat gtggcggagc acgtagcctg cggtcccgat
     6361 ccggaggcgc acatcaaaaa gatcgaggag cgcatcagga tggggttcga ccacgtctgt
     6421 gtccaccaga tcgggcacca gcagcgggag ttcatggagt tctacacgaa cgaggtcctg
     6481 ccgcatttcc gggagtgagg gcgttacagg agggttttgg aggcacaaac ccgggcgagg
     6541 gcgaggcgcg ggggtagaga cagcggcatt tacgggtcgt gacaagtaaa gcatctaaac
     6601 ggtggaaaac ccgatgcagt ggaccgtggt gaatatcgcc attggggagg ggctgacggg
     6661 gagggggggg gcgtgccccc ctcccctgtc tacaccagat aggcgatacc tgtaaccccc
     6721 accccgcccg gccnncctcc ggcctcctcc cccgccccct tggggacggg ggcagtgcgt
     6781 ggcgacaccc catggaaagc cgtgcatgag ttgtgattgg ccggaggaca cgaacccacg
     6841 catcagagga tgacgttcct ccaggaacgg agtttgagca ccgacagtgt aaaatagcga
     6901 cggaccagag gtgcgaaagg cagtacgtct aaagtgtgaa cgctgtcaga tgccaggcct
     6961 cctccgcgca aaggcggccg cgaaacaacc caaaaaaagg ttatgggtcg gccggatcct
     7021 cgagatccgt ccgcaccctg cagatgctct cgatcggcag gacgaagatc ctgccgtcgc
     7081 ccggcttgcc ggtccggccg gttgcccgga cggtcctgat gaccttctcg acatcggcat
     7141 ccttgacgac catctcgatc ttcaccttcg ggatgagctc gaccggcatc gttcttcctc
     7201 tgaactggag ggtgatgcct ttctgcgtcc cccggcccat cacctcgctc acggtcatcc
     7261 cgttcacgcc ttccacctcg agtgccgctc tcacggcatc gaacatctca ggcctgatga
     7321 ctgcagtaac catcttcatc aacaccacct cacacccgca cggactcgcc gtgctgggcg
     7381 atgtccagtc cgacatactc ttcctcttcg gtgacgcgca ggcccatcac agcatcgatg
     7441 accttcgcga tgacgtaggt cgcgccgaac gagtaggcca caaccacggc cacatccacg
     7501 agctggatga ggaactgacc cgggttcccg tagagcagac cgtctacgcc ccctattgcc
     7561 gcgaccgcaa agaccccggt cgcgagggcg ccgaagatgc cgcccatacc gtggatcgcc
     7621 catgcatcga ggctctcatc caggcccctc ttgacccgga agagcagggc cccgtagcag
     7681 acgagaccgg cagcggcgcc gatcgggatg gcggccatgg gcgtaacgaa ccccgctgcc
     7741 ggagtgatgg cgacgaggcc ggcgatggcc ccgctgatga tcccgaccga acccggcctg
     7801 ccgcgaaccc aggcagcgca gagccaggag agagcccccg cggcggcagc gatattcgtc
     7861 acgacgaacg cgtttgccgc aaggccgttc gcggtgaggg cgctgccggc gttgaacccg
     7921 aaccacccga accagaggag cgccccgccg aggaggacca tcgggatgtt gtgggcctcc
     7981 atgccgtact gcccgaatcc gacacgcttg ccgatgacga gcgccagtgc gagcgccgag
     8041 aagccggaac tgatatggac gaccgttccg ccggcgaagt cgagcgctcc aagctgcgca
     8101 gcccatccgc cgccccacgc ccagtgggcg agcggatcgt acaccagcgt cgtccagagc
     8161 aggccgaaga tgatgaacga actcagcttc actcgttcgg cgaccgccga cgtgacgatg
     8221 gccagagcca gcccggcaat gaccagctgg aagaccatga agagcaggtc cgggatgccg
     8281 tcgccctcca tcccgacgcc atgcaggaag acgtaatcga ggtttcctat cagcccgttg
     8341 atatcggtgc cgaaggccag gctgtagccg aagacgaccc actggatgct gacgagggca
     8401 aatgcgatga acgccagcgc gagcatggag atgaagttct tccgccgcac gagcccgccg
     8461 tagaagagcc ccactccagg agtcatcagc atgaccatag ccgtgcagat gaggacaaac
     8521 gccgtagagc cggtatcaat catgccccgg cctccgggac gcccgtggat agattacgta
     8581 ggtagccgtc accattcatc ggaaggaagt cgccttccta gagttataaa tttatctcgt
     8641 acggagagaa cgtagctgca ccccggttct gtttcaccga acgcgtcatg gggatagaag
     8701 ttcccccaga atacggcccg gaataccccc gggcggatcg ggttcgtgca gagaactgca
     8761 gggccccgac cccctgcgtt acagccctgc tcccgacgta cgagggggga cgatccacac
     8821 aaaatagaaa gacataaatg cgcaggaagg aaccttcctt ctaccatatg cgatgcaggt
     8881 gaatatggtg aatctgaaac acgtacacat tatgctgatc gtcgtcctcc tggcgatgct
     8941 ggtcacgccg gtccttgctc aggacccgac cggggccgag accctcgagg gcgatccaaa
     9001 cgcacccgtc aactttgcat ggaccctgat ctgtggattt ctggtcatgt tcatgcaggc
     9061 cgggtttgca atggttgaaa ccggactgac ccgggcaaag aacgcctcca acatcatgat
     9121 gaagaatctc gcggacttct cgttcggcgc cctctcctac tgggcggtcg ggttcgctct
     9181 gatgttcggc accgcctccg ggatcaccgg gctgctcttc ggcacggacg ggttcttcct
     9241 cctcggggac gcctacgacg tgagcaccat cgaactctgg ttcttccaga tggtctttgc
     9301 ggcaacggca gccaccatcg tttcgggtgc ggtagcggaa cgggtgaagt tctccacgta
     9361 cctgatagcg agcgtcgcca tcaccgcgct catctacccc atctacgggc actggctctg
     9421 gggcggcggc tggctgaaca ccgcagactt catggtcaac ctcggcggcg ggtacggtgc
     9481 gctcgacttc gccggttccg gcgtcgtcca tggcatcggc ggtttcatcg gcctggcagg
     9541 tgccctgctc ctcggtcccc ggatcggaaa gttcagaaaa gacggcacgc ccgtggccat
     9601 cccggggcac gccctgacgt ttgccgtact cggtgtcttc atcctctggt tcggctggtt
     9661 cgggttcaac ccgggaagca cccttgccgc caccgagctg cggatctcgg tcatcgctgc
     9721 caacaccgta ctggcttcgg ccgcgggtgc gatcaccacg ctgttcatca cctggtggcg
     9781 gttcggaaag cccgacgtca gcatgaccgg gaacggggtt ctcggcggcc tcgtcgccat
     9841 aaccgcatcc tgcgcgtggg tgagcccatg ggcatccgtc gtcatcggca tcgtcgccgg
     9901 catcatcatc tacgtcggcg tctggctcct cgactgggtg ctccacatcg acgatccggt
     9961 gggcgcggtc tccgtacacg ggttcaacgg cgcctgggga ttgctcgcgc tcggcctctt
    10021 tgcagacggc acctacggtg tatataccac cgaaggtccg ctggtgaccg ggctgctcta
    10081 cggcaacgcc ggattcctgg cagcgcaggc catcagcatg gcggtgaact tcgcctgggc
    10141 attcgggcta gggcttatcc tcttcgccgc cctcaagtat acggtcggca tccgggtctc
    10201 tccggcagaa gaactgcagg ggcttgacag cggagagcac ggggtgagca cctatccgaa
    10261 cttcgtagtc gcggagccca ccctggacgg tgagaaagca tgaagaagat cgaagcaatc
    10321 attcggacaa tgaagttcga gggcgtcaag cacgccctcg aagatgcagg attcgactcc
    10381 atgaccgtca ccgaggtgaa gggccgcggg cggcagaagg ggatcgtcca gcagtggagg
    10441 ggcgccgagt acgtcgtcga cctgattccc aagacaaaga tcgaggtcgt ggtggcggat
    10501 gccgatgtcg agaaggtcgt cgacgtcatc gccaccagtg cggcgaccgg ccagatcgga
    10561 gacgggaaga tcttcatcat accgctcgaa gatgcgatca gggtccggac acgggagcgg
    10621 ggtgatacgg cactttgacc aacgcaccct acacccacat cccccatgtg attgcatccg
    10681 gggccccctc aagcaagtca gagggcaagt atgtgcccga tgtccgggca ccaacccctt
    10741 tttacacgtc ctcccttttt tcagggcggc acaccgtgcc cccgaactag ccgagacacg
    10801 aacacaccac ccttatggcg tgatcagggt atggattgat gcatctgaag atcaagatct
    10861 acccgggtga tacgatgagc acgcatcgga gtccggcagg catccagcgg gcctgcgccc
    10921 gcctgcggtc ggcaaagacc cgggcggaac ttgcagatat cctctaccgg gaggtcgccg
    10981 gctactcgct cttcgatctc caggcgatgc gggggagggt ggagcaggac ctcaggtccg
    11041 tccccgcggg ttaccggcgg cgcctctatc cccggatgat ggagatgatc tttgagacgc
    11101 atcacaccct catcgccacc atgcgccgga gcggtctgga agtgcaggac gagccgctca
    11161 cggaggagtt cctggaatac tgcacaacgg tcgagagaac gtgccttgcc cgggacgagg
    11221 aggagcagca cctcgaactc ctctacttcc tccttgccgc cttcaacatg ttcgtcctcg
    11281 gactcccagg ccacccggtg ggaacgccgt tccccggcgg gatcgaggtc gaaatcaggg
    11341 gcggcgagta cctctgcccc atccgggaga aggccgacga tgtcgagaac gccctctgcc
    11401 cctactgccc ggctaagcag agcgacgatt gaggcgnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccggga
    11521 cgttgaggag tttccggaca tcctcggcgt agtccttctt ctcccccgca acccagcagg
    11581 acccgacccc ccatgcctgg agcgcgagga ggagctgcat cgtcgccgca cagcagtctt
    11641 cgaggtagta cttcgcatct ctcttcccga agacagcgaa gcagatgggc gcatcggcga
    11701 tgaaccggcc attctcggcg aggtcagcga tcgcccgcag cgtatcgcgg ttctggatgg
    11761 tcccaaagag ccagggctgc tcgttcctgg cggtcggggc aagacgtgcg cagtcgaggg
    11821 catccttgat ggtcttctcc tcgatagggt tgtccttgta cttccgcacg ctgtgcctgc
    11881 tccttatcac ggtgacacca agattaaccg ttccaccaat cggactcatg caacgatggt
    11941 gggcactcca ggcctaaata ccttggggaa aatcggggag cggccttaga agaggccctg
    12001 tatcatctgc accagatttg agaagccgtc gacgatgacc acggccggat cgatcccgag
    12061 gaccgggaag atgaatatga agtaagcgac ggtcccgagc gtgctcccga cgttggcgag
    12121 agcagcgacc agcaccaccc ggaagagcgg gatcttccgc atctccgaga aggtctccgc
    12181 ctcgaggatc ttgcgcaact caccggcggc gggcttcctg atcttcgcct cgacgatggc
    12241 cgcaaaccat cccgccgcaa gcagcgggtt gagcgacgtc agccaggata tggcaaatgc
    12301 cgtggcggcc gagatgggat gcccgccggc gagcagcgtg aatgcggcgc tcagggcccc
    12361 gttgatcaag acccaataga ggagagcggt caacagaacg tcgaacccca cacccgagaa
    12421 ggctatcgcg gctatcagga gaaggaagag gacggtgacg cccaccccga ggatctttgc
    12481 ccacggcagg cccttcgcgt cggcggtcag tcccggaagc ggcggcagcg tctccggcgc
    12541 gtcaaggtaa cgctgcaccc cccggatatg tccggcaccg acgaccgcca gcacccgttc
    12601 gtaccggcct ccgagcatca ggatctggtg agtgagatag gcatcccgtt catcgatcag
    12661 ggcctgagca cccccgggag agaacttccg gaactcctcc atcgcggcac tcaccacatc
    12721 ctggttcgtg aactcgtcta cgtcgatctt ctggccctcg acactcacca tggagtacac
    12781 cagggccccg aggagcttga tcttctccca gattcccatc atgccccaga accgggcaag
    12841 cgtgatccgg atatcccggt cgatgagcgc cacgggtttc tggtgcgcct cagcctcctc
    12901 gatggcgacc agcatctcgg caccgggttt cacaccggtc tcgacgccga tccgctgctg
    12961 gatgtaggta agcacccact ggacgaggag cctgccgaag ttcccgcctt tcaggatatc
    13021 cgtgaccgag ggctcggcca cctcctgcgt gagcgatatg aaccggcccc ggtcgagttc
    13081 gacgccgacg atgtcgggct ggaactcctc gatagtggat cggacttctt caacgctctt
    13141 ctgcgagaca tgggccgtcc cgacgaggcg gatctcaacc attgagcacc tttacacctt
    13201 cactgtcgat gacggcgcgc ttccccggcg gcaccgtcgc ggtccggtag acagcacgga
    13261 gaccggcctc ctctgccgct tcggctgcct gccgcctgca gaggtctgcc gcagcatcgt
    13321 gcgcgtcgcc gggagagagg ggtgtgccga ggccggggca ttccgagacc agcagatcgt
    13381 cgtccaccag cataaacggg taggtacggc agatccacgg gcgatgggca tagaccgagc
    13441 accgcccgtc ccggagaaag atacaggcat cagccgttcg cctgatgcac caggcaaagg
    13501 tgtactcccc gccgttcccg gcatcgatga agtcggggta gggcccggcg acctcgtccc
    13561 aggccatgcc ggtcgccgcc atgattgccc gaacctcacc agggctcacc agcacgaggt
    13621 tcgagtcctc cgcgacccgc cggcagcagg caccgcactc ccggcaatgg aacccgaagg
    13681 accccacccg gttcgcaaca tcgtctactc caggcatatc cggtcaaaat tctcaaagag
    13741 gagccgcccg ttgacggtat ggctgacctc cgggtgccac tggacaccat agaggcgctc
    13801 atcgggagat gccatcgcct ccacaccgca gatgaccgac tctgcaagcc ggacaaaccc
    13861 ttccggaacc accgagacct catccgcatg agacgcccat acattgatcc ggttcgggta
    13921 gccctggagg aggctactct gctcgaggat atctacctcg accgcaccga atcccccact
    13981 cgccccctgg cggactgcgc ccccccgggc cgtcgccatg atgtgcagtc caagacagat
    14041 gccgaggacc gggaggccaa ggtcaaggta cgcaggagcg accccgatgc ggtccagggt
    14101 cgggccgccg ccgaggatga tgccgcggca gccccgggcg acctcggccg gcggcgtctc
    14161 attggagatc atcgtggtct cgaggtccat atcacggagc gtcctcaaga tcaggtggtt
    14221 gaactgtccg tgattgttga ccacatagag tggaagcatt gcgtataggt cgatgtaaaa
    14281 gggtatagtc cttccctcag gcatactttg tgacgaaatc aaagactttc cgggtgatct
    14341 cctccggatc gtggcccatc aggtaggcga gcgtcagagc cccgccggca cccatccctt
    14401 ccttgacctc accgatgcag tagcgggcga gcccgacgtg cccgataccg gcgaagttcg
    14461 ggtcgacgaa gtacgcggtg gtgccgacat cggcgaccga gcggacgaac ccggcggtgg
    14521 ggtcgtccct gacgtagatc gtcgtcgcca ggtgcggcac ccgtctcccg agcgccttta
    14581 aggtagccgc gacggcgagc atctgggtcc cgccggcaaa gaggatatcg ccggtatacg
    14641 cgctcgcgat ccctgccgcc accggcatca tggggtcgcc cgcggcgcgg aggatctcaa
    14701 ggggccctct cgcgcccgtc tcgtctatcc gggcgagcac ctccctgcag acggcgtttt
    14761 tcaggccgac ggggttctcg gcaaaggcac tgctgaccga ggcgtcgtag ccgagcgccc
    14821 gaaggacgca gagagcggtg gtcgtcccgc cgggaacgca ctccccgagc atcaggaagt
    14881 cgctgtactg cgagagaagc cggccgaccg tctcgccccg ctcaaagagg agagcggcat
    14941 gcggcaccgc gtcctccttc cggggatcgc cgccggggct tccgtagacg tccaggcagg
    15001 ggacggtggg agtgtgggcg agccccgcgt tgatgaggac aggggcaagc ccggtcagtt
    15061 ccatcatgga ccgggtgatg gtcgccggtg tcgggcaccc ggtaggcgta ttcggccggg
    15121 ccgggacgct cgtgatcgcg ccggtcgtga cgagctccgc gtccaggacc ggggtgagca
    15181 gggtcttttc cggggtcggc ccggcgcccg atatgccggg gacggtggag agcatggtgt
    15241 tgccgaggac gagcgccatc atcgggcggc atggccggaa ccctggatct tctgagagga
    15301 acgggtgaga catcaggtac agggtaggtg ccgcaggata gaagagattg ctgtcggggg
    15361 cgtggcggga cggggtgcgg gtgtcgggtg tgagcgaggg ggggcgcagt tgccgggtat
    15421 gccggagcgt cgccatcgaa aacccaaagg gtttttctcg aactccggcc ctttggcccg
    15481 tcgttaaatc caaacgccaa aatacctccc ccctcaatac ttgaacaatg ttcgagatag
    15541 agcgcaggct ggaagagaaa ggtatcaaga ggggagatat cgtcgcctcc gcgatggaac
    15601 tctacgtctc ccacgggata ggggaagaag aggcttcaaa acggctggat aagaagatcg
    15661 ggaaggcgct ggaggacccc aacgtatcat cgctcctcct cggcgccatc ctgctcgagg
    15721 acgaactcta cgtgaagcgg aagaactccg agatcgccga cgaccccgtc ttcctcctct
    15781 ccgacgagat catcgggatg gccatcgccg aggtgatcgg ggggacctat gcgcggttcg
    15841 agttcacccg ctacgaccag aagaaacccg ggatcttgag tatactcggg ccgttcctcg
    15901 acgatgccgt cgccgggctg atcgcgggct gcacgtcacg cctctacagc gagtcgatgg
    15961 gatcgatgtg aagtccgtca ttgccctcct gcagttctgc accccgctcc ctctcgggag
    16021 acctgtcgag ttcgagcact tcgcacgccg ctcctacctc tacccgctcg ccggatacgt
    16081 catcggcggg atagcggccg gtatcgcctg ctggatagcg tcgccggtgg tgggtgcggc
    16141 cgtcgccctc gccctggtgc tccttctctc cggatgcaat cacttcgacg gcctgctgga
    16201 cttcggcgac gggctcatgg cccacgggag ccgggagaag aggatcgcgg ccatgaccga
    16261 ccggaccacc ggggccgggg ccgtcgcggc ggggatcgtc gtcacactcc ttgcgtttgc
    16321 cggactccag agcgttgcat acctcccggc ggccatcctc atcgccgagg tctgcgcaaa
    16381 ggtcgcgatg tcctgcctca ccacgctcgg cgtgccgttc cgggagggga tccactccta
    16441 ccttcacggg tttgcacgac cgtggttcct cttcccggcc atactgctcg cgctgcccct
    16501 cttcctgctg ccggtgcccc gggcgagcat cgcgctcgcc ctcgcggtca cattcgttat
    16561 cgtcgcactg atgatgcttc tctcccggcg gctttttggc ggcgtcaacg gcgacgttgt
    16621 cggcgccaca aacgagatca cccgggcggg ggtcatcctc ctccttgccc tcctgtaatc
    16681 acctttttca tcccggcgac cgctactact gtagaatgaa gcaagaccat actctctact
    16741 cccggggagg gatcatcacc gagcggaacg cagactacgc aacgatacgg ctgcgggttc
    16801 cggcaggggt tctctccgca gcgcaggtca agcagctcgc caggatcagc gagaagtacg
    16861 gggacggctc gctccacctc accatgcgcc agacggtgga gatcccgcac gtgaaccccg
    16921 agaatctcaa gaagatcgtg aaagccctcg ataagaacgg gacgcccatc ggagcagagt
    16981 ataacgaagt agtgaatatc atggcctgcc cggggacaga gcggtgcaag tacgccaact
    17041 gcgagacgat cgaccttgca cggaaggtcg acgaacgggt cttcgggaag gaactcccga
    17101 tacggctccg catcgccata tccggctgca cctacatgtg caacagccca ctcttgaacg
    17161 atatcgggat catcgggagg atcaggccgc tgcgcacccc cgggctctgc acgggatgcg
    17221 gcacctgcgt ggagtactgc aaacagtgtg ccatagtgtt gaaaaacggc atatcagtcc
    17281 ttgatgaga
//
LOCUS       DAJB01000177            7561 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_107200, whole genome shotgun sequence.
ACCESSION   DAJB01000177 DAJB01000000
VERSION     DAJB01000177.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_107200"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1294 a         2341 c         2561 g         1365 t
ORIGIN      
        1 ttcctccccc ggttgaaacc gggggtctcc ttgcccgtcg tatatgagca tttgcatccg
       61 gcggccgggg tcaccacccg ttgtggtgga cccggtcctc gcggtagcgg tcctcgggcg
      121 ggggacggac ggccgggtag ccgatggtga caagggcgag caccacgatg ccgtccggga
      181 gcgaaaagag gttcgtaaac ccgagcatca ggtcggggtc cgggtagacg gcggtccaga
      241 ccgcgccgag cccgagggcg tgggcggcga ggaggaggtt ctctgtcgct gcggcgcagg
      301 cctgcacccg aagatccggg ctctcggggc ggcggagatc gatgcatacg aggatcgcga
      361 gcggtgcggg agtgctgatc gccggtatgc gggagaggat ctccgggtcg tctatgacga
      421 tgaaaccggg cttcttcctc tggttcatga aagtatcgga gcgcccccgc cctaccgctt
      481 cttcttctcc cggcggatcc tgacggacga gaaggggaac ttcacgaagt cccggaggcc
      541 ccctgccggg gagaagttcc gcatatcccg ccggaccccc gcgatgagtt cgtcgacgac
      601 ctcctgctcc ttcgcctccc cctcctcctg caccacggag aggcggtgga gggagtagag
      661 cccgatgatg aaggcgaaga ggaagaagaa ctcccactgc tggacgtcga gagtgacgaa
      721 gacgagttcc tgcaccgggt ctttccagat cagggtccag atcagttctc gctcggcaaa
      781 gaagtccacg aagagcccgc cgaggatcgg ggcgacgccg gcggcgaccg agttggcgaa
      841 ggtgctcgct gcaaggtaac cggttgcctg gccctgcggg gccatcttca ggctgatgtt
      901 tcccgaagca agcgagacac ctgcaagcga gatccccatc aggatgtgga tgagtatgag
      961 cagggggtag gtcaggacgt agacgtcggg gagcgtgacg aacatccacg cgaagatcgc
     1021 gagcatgaaa agcgggccgc tcacggcaag gaccgacttg ttggagtacc ggtcggagac
     1081 ccgaccccat gaccggtaga agatgatgtt catgatctgg ctgagaatgc tgagagcgat
     1141 gacgagtgag acctcgaggc cgatcctctg cagcatgtag accgtgaaga acggcgaggc
     1201 aaggttgacg gcaaagttcc acgacccgag gaagatgatg aggtttttga agttgaggtc
     1261 ctggaacggt tttctgatgg cgacgaacag accgtcctcc ccgtcgtcga cggctatccg
     1321 gggctcgggc gtgcgggcga ggaacgtaat cccgatgagg ccggcgatga gcccgaggag
     1381 gaagaggatg gagtagccgt aggctgcaag gtccgggaag acggcctccc atgagtcgat
     1441 gaagaaaccg gcgacgaggc tgatgacgag cgcaattacc gtggagaggg tcagccggtg
     1501 ggagaagaaa ctcccgagcc tgtcctgcgg tatcaggtcc cgcatccagg agttccagcc
     1561 gcagtgggag accgccgaga acgacgcgta gaaggcgatc gatgcgatga gcacgaatat
     1621 cccctgctcc ggtgagagga agaacggtat cgcgatgatg ggcatccagc agaggcgggc
     1681 ggcgagtgat gccgcgacga ccaccagcct cctattgcgc acccggtcga cgatgtagat
     1741 ggcgggtatc tgcaggagct ctgcaagcgg ggggatggcg gcgagcaggc cgatgacggt
     1801 attcgatgcc ccgagctgca gggcgaaggc tacgaggaag atgccgccgg tcagcgtcac
     1861 catcgcctgg gtggtaaggc cgtccctgat gacgagtttt aacccgtgac cgacctcttc
     1921 gtccgtcagg ctctcttttg tatcaaagac catgctccac cggtgtaccc tcttctgacg
     1981 ctcacggaac cgttcacctc gaagggccgc ccggcggcag gggaatccct gccggaactc
     2041 ccgcgtcgga tcctgcggcg gacgccgttt ttcgggtctt attgtataaa aatagaatta
     2101 tattaatctg tttgaataaa tcccgcgacc ctccggggtc ttccggcggg gaccccggag
     2161 cggttcaggc aattttgggg tagactctct gtggtaactc ccccggggag ctgccctggc
     2221 tccctcaact ccccgacgac cacggcgtcc tgaagtcggc acagagggtc agcccgccgt
     2281 ccacgtagag cgtctgcccg gtgatgtaag cggcttcgtc ggacgcgagg aaggcgaaga
     2341 ccgcggcaat ctcctcgggc tctccggcgc ggcccatagg gatgtgcccc tcgacaagcg
     2401 ccttcttctc ggggtcgtcc gcccactccc ggtttagcgg ggttctaatc gcgccggggg
     2461 cgaccgtgtt gacccggatg ccttctccgg catactccag cgcgagcgtc ctcccgaggg
     2521 cctcgagtcc cgccttgctc gcggcgtagg gagcatactg cggtttaggg acggtttcgt
     2581 ggacggaggt gttgttgagg atgacaccgc cgcccccgcg gtcgaggaag tgccccaccg
     2641 cttcccgggc acagaggaac gcgccccgga ggttcaccgc caggacccgg tcgtaagcgt
     2701 ccgcagtcat ctcgtgggtc gggctctcgg tctggatgcc tgcgttgttg accaggatat
     2761 cgagccgccc ccacgccttg aggacctccc ggaatatgcg tttgacgtcc tcttcccggg
     2821 cgacgtcccc ccgcagccct tctcacggat gagactgcag gtcttccggg tctcctcaag
     2881 ggttatctcc gcttccctct cgctcgtgtg atagttgatg gcgacgtttg ccccatcgtc
     2941 tgcgaacctg atcgccgtcg cccgaccgat gccggttgag ccgccggtga cgaggacgtt
     3001 ctttccttcc agccgtttca tctcctgccg ggttttgggc ggcgccgatc ggagcccgct
     3061 ggggttgaac gcctgcggga atccggccgg gcatcccgcc cgtcccgggc ccctgtggtg
     3121 cgggtgggtt atgaaggtct cggccggggc tcaccgccgc tcctcgagca ccttccgggc
     3181 gagcactctc cggtcgggct tcccggccgt gagcgcctgg aacttctcct cgtagaccgg
     3241 tttttcgatc gagtagaacg tcccgagcgg gatcggcgca tcccggtcgt agtcccactc
     3301 ccggatcttt ccaagagcgc tctcgaatcg gccggtatcg acctcgccct cctgcatctc
     3361 gtaagcgtac tcgttatagt agtcggtcag gttgaagtag gtcgcgcaga tctggaggac
     3421 gtcgacgaac gagaacccgc ggtgcatgat cgcctccttg aagaggtctt ttacgtgccg
     3481 gatcttcttc gtgtagcccc gggcgatgtg ggtggccccg gcagcgagca tgacttcgag
     3541 cgggtttaaa gggtgctccg ttatcccgcc gggggtggag cggcccttga aaccgaaggg
     3601 cgacgtcggg gtgtactgcc ccgtcgtcag cccgtagacc cggttgtcgt ggacgatcac
     3661 agtgatatcg ctgttccgtt tggccgcgaa gatgaggtgg tcgagcccct cggcgaagat
     3721 gtctccgtcc ccgacgtggc agatcacctt gagatccgga tttgcaaggc gtatcccggt
     3781 ggccgccggg accgaccgcc cgtggatgcc gtagaaactg ttgacgttta agcagtcgac
     3841 cagtttcccg tggcacccga tgccgccgag gaggacgaag ttctcgagcg gtatcccctc
     3901 ctcctccgag agttcggcga tggccgactt catcatgtgc tctatcgaga agttgccgca
     3961 cccggggcac caggtgttct gcgccgggct gatgagcccc ccggccggtt gcccggcagg
     4021 ctgctgtggg ctcatgcgaa gacctccaga agcctcgcct ccagttcgtc gaccgcaaac
     4081 ggccgcccgt cgtgcttcag caccggccgg ccggggtcga agccctgctg ccggaggagg
     4141 cgggcgagct gcccggtggc gttgttctcg acgcaggcga ccttccccgc cccgatcatc
     4201 gcctccttcc attgtcggac ggggaacggc gcaagaacga gcggctggac gacccgcgcc
     4261 ccgaacttct cggcggcctc gatgcacgcc gacttctccg acccccagca gacgatcgtc
     4321 ttcccggcgt cccgggggcc gtagaccctc accgccgggt aagtgctgag ttctgccttc
     4381 aggctctcgc ctttccggaa catcttctcc tgcagcgccg tgacgtcctc taccgtctcc
     4441 gtcgtgaacc ctatctcgtc gtgggcgtaa ctggttgcct tgacgacggc gccttccctt
     4501 cccgggaagg cgagcggcga taccccgtcc tccgtccggg cataccgccg gtactcccct
     4561 gcaccgtccc agagtaccgg ctcccggtca gggggctcta tgacggcccc gacatcgaag
     4621 gagtaggctc cttccgcgag cgtcttgtcg gtgaggatga tcgcggggac ctggtacctc
     4681 caggagagca tcagggcggt tgccgaccag gcgtaggcct ccgcgaggtc gcccggggcg
     4741 acgacgagcc gggggaactc cccctgacct gcgttcagga cgaagtggag gtccccctgg
     4801 gcggtgtagg tcggcatgcc ggtgctcggc ccgggccgct gccccatcac gatcgtcacg
     4861 ggaatctcgg acatccccgc aagggagagc ccttcggtca tcaggcagaa cccgccgccc
     4921 gaggtgccga ccgccgtctt ctcgccggca taggcgagcc cgagcgccat caggatgacg
     4981 ccgatctcgt tttccgggtg aatgaccttg atggcgaggt cttctgcgtg cccggcaagg
     5041 aagtgaagaa ggctcgacga cggggtcatc gggtaggcta cgtaggactc aagcccgccg
     5101 tggacgagcc cgagccccgt gacctcgttc cccgtcagga ccgggagcgc cggggcgctg
     5161 agcggctcga cggtgaagac gtctcctgcg gcgtcgtaac cccgtccggc gacccggagg
     5221 tttgcctcca ggtgctttgc ggggacgctt ccgcgaatga cgtcatcgag gacctcccgg
     5281 ccgatgcccg ccacccgggc gagcgccccg agcatcgcgg agtttcccgt gatcgggggc
     5341 gccttctctt cttcgacgat ccggtcgagc gggaggccgt agccttcggc ccgcaccacc
     5401 tgcgaggcgt cgtagatgac ggtcgtgccg tcgtggaccc tctggcggtg gttatcgatg
     5461 ctgttctggt cgaaggcgag gaggacgtcg accggagtgc ggtgcgcccc gatggtcctc
     5521 tccgaagccc ggatgatggc gaagttgtgc ccgccccgga tgagcgaggg ataatcgaag
     5581 tacatgtagg tgcggtagcc gaggcgggag aagaggccgg cgatggagag gcctgccgtg
     5641 ttgatgccct ccccggcctt cccgccgatc agtacggagt attcggccat ggtgatcggg
     5701 catggcgtag attggctggc ggtatatagg ttgtgtccgg cctcttcacc cgacaaccgg
     5761 cgacgccgtc tcgtcgaagg ggttgctccg catcgcaagc gagaagaggt aggggtagtt
     5821 cttctggaga tactccatgt agccgagcca ctgcccgacg agcaggctgt acacccttgc
     5881 gatgtctccg ctgaggtggg cgatgtcgct ctccgggagc ccggagaagt cctcccgccg
     5941 ttcgagttcc tcggtgaggt ggaataccgc ccgaaggagg tcggtgaacg attcgtgctc
     6001 caggaggacg gggttctcga gcaggcggag gtggaagccc cgccgctctt tgaggtaccg
     6061 gcagagttcc tgcaggtccg cggcatcgac ggcgacactg caggggtgat gctggaggcg
     6121 atcacgcacc gcggagaaga tctccggtgt ccaggcgtcc gtcacgacga ggtcctgttg
     6181 gacctccggg aggcgcgggt cgtggtccga gaggaaggtg aggagcccgg tgccgacctc
     6241 cgagaagaag gtcccgatga ccatgttcag cttctccagc ctctcgcgct ttgcccggat
     6301 actcaggagc tggtttaaga tcagcgtgac caggaggacg tttatcggca gaaacccgag
     6361 agcgttgaag atgtactggt aggtattcac cggatcgccg agcaggaggt acttcgcggt
     6421 gtagacggcg gctgcgaagg cgatcaggac gatcccgagc cgtacttccc aggtccggag
     6481 gctgaggcgc atgatacaga taagatacgt caggtgggaa aaataaaggt tgggccccgg
     6541 aagcccttca gaccgggagg cgcgacggcg ttggcacctc aaggggtgcg ggaaccttcc
     6601 gccgctctgc gatctccgcc tcgacccagg cggccaggtc gagcaggccc gcgtcgaggg
     6661 tgacctccgg ggcgtagccg aggacttccc gtgcacggct gatatccgcg atacagtggc
     6721 ggatatcgcc gatacggccg gtccccgtga cccccgggga gaggtgctgg cgccgggtta
     6781 tcgtcgcaag aagagtggcg acggtccgga acgtataggg gcggccgctc cctatgttga
     6841 acgttccgcc ggccgcggag ggcgactcaa gagccaggcg gaaggcgcgc accgcgtcgt
     6901 agacactcac gaagtcacgc tgctggtagc cgtcctcaaa gagatgcacc gggagatcct
     6961 ggagcaggcg ggatgcatac tcggtcagca tgccggagta ggggctggcg tatgcctggt
     7021 gcggaccgta gacggaaaaa agccggagaa tggtcgtcgg gatgccgtat gtccctccga
     7081 tcagcctgca catctcctcc tggtcgtact tggagagggc gtagaccgag agcggcgacg
     7141 cctcctttgt ctccggggtc gggaggggat agatcacctc cccgtccggg ctccggggct
     7201 cccagtcccc ccgtgccacc tggtcgggcg agcgctggat cttcgggtaa atggtgccgt
     7261 tgtacgagca gtataagcct tccccgtaga cggcgctgct cgaggcaacg atgagccgct
     7321 cgaccggacg gtcgagcagg gcctcgagga ggacggcggt ccccgtggtg ttgacgctca
     7381 tgtacttctc gatccggtac atgctcgacc gctccccgac gaccgctgcc aggtgaatga
     7441 cggcgtcgac tccttccagg gcctcccgta gccggtgcgg gtcccgtata tccccgacga
     7501 agacctccgc ccgccggtcg agatggtcgg gccgctgcct ctccggccca tgcacgcgtg
     7561 g
//
LOCUS       DAJB01000178            6342 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_52173, whole genome shotgun sequence.
ACCESSION   DAJB01000178 DAJB01000000
VERSION     DAJB01000178.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_52173"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    264..375
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1343..1393
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1189 a         1891 c         1929 g         1170 t
ORIGIN      
        1 tttcttgcgg gacgtgagat caagagatat cttactccag accccacgaa gtttctgatc
       61 ctctttgaaa aagggtggac taaccagcac tcagggagtg tatctgacaa atgggactgg
      121 ctaaaaacct cctatccggc gatcgcattg caccttgagc catttgcagg aagtggtgaa
      181 cggaggtgcg ataaaggaga ctattggtgg gaactgaggg cctgtgacta ttatcaggaa
      241 tttgagaggc cgaagattat ctannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnccccg catgagaagg aggtgctcgc cgggatgatc gacgcgaccg
      421 accgggagat cgataggctg gtgtatgagt tgtacgggct cgcggaggag gagatcgcgg
      481 ttgtggaggg ggctgtctga tgcgatgaac ccgctcagca gatggactgt taccggttcg
      541 acggtgagcc gtgagggaag catgccggga gaaacgtcgt taccgggagg gtgaatgcct
      601 gccggcacaa ccctgcgacc ggcaacccgg gggaaaaacg ttatcctgat gggcgggtaa
      661 caggagtatg atggggtatg cgcgatggtc cggtaggaat gacgctccgc tccgcggagt
      721 tcgaaaagac cgaaggtctt cgaggaccgg agcgcgagca ccgccaggtg cgagtcttac
      781 ggctgatggc atcctcggtg cgctggcgga acaccgcgaa cggatcagga gcctgggcgt
      841 tcggcggatc ggggtcttcg ggtcgtttgc ccggggcgaa gagcgcgaag agagcgatat
      901 cgatatcctc attgagttca aggaaggagg gcgctccttt gacacgtaca tggacctcaa
      961 gttcttcctt gaagatctct tcgggagaag agtcgacctc gtcgaccgcg atgccataaa
     1021 accggctctc gcaccgcata tcctccggag tgtccggtat gtcccgggaa tataacctct
     1081 atctccacga tatccttgag gttatcggga aaattgaacg atacacccgg ggaatggact
     1141 acgaggagtt cctggcagac gatctggtgc aggacggagt gatccggaac ctcatggtca
     1201 tcggtgaggc cgtgaaactc atccccgaac cgctgaaatg cgaacacgcc gatatttcct
     1261 ggagaaagat tgcaggaatg cgagacatcc tcattcacgc atacttcggg atccataacg
     1321 agatcgtgtg ggacgttatc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnntatcagg aacaagatcc cggaactacg ggttgcagtc aggcagatgc
     1441 ttgatgacga ggccgcctga ccgccctctg ccgtggttcg gtgcccgaaa acggcttgcc
     1501 ccgccgacgt cgcccgccac gaccgcatgg tcgtcctcgt agaacaggtg ctcgacctga
     1561 acaaccgcct cgcggcggcg aaggccccgc acgagcggga ggtgctcacc gggatgatcg
     1621 acgggaccga ccggcagatc gataggctgg tgtatgagct ctacgggctg acggaggacg
     1681 aagtgaggat tgtggatggg gagacacgcc ccgaaccaac ctgcaccgcc atcccggcct
     1741 ctacagcgcc ggagaggcgg aaagagatct ctcctcgtct ttgaccagcg ggtaattcgc
     1801 cgcctcccat ccgatggtgc cgccgagcag gttggtgacg tgccggaacc catgcttctc
     1861 gaggatgctt gccgcaaggc ttcccttgaa cccggcatcg cagtggacga cgatctgctt
     1921 ctcctccggg acctccgcca ggtgctcttc gagctctccg acgtagatgt ggtgggagcc
     1981 cgcgatgtgg ccatcctcct cccggtggtt gacgttcctg acgtcgagaa cccagaccga
     2041 cgggtcgtcc aggtgctccg cgagatcgga gggggaccag gtgccgttcc tctcgatctc
     2101 ctcgccctgc ttgaaccagg ctgcaaaccc gcccgcgagg tatcccgcga tgttgtcgta
     2161 gccgagccgc acgaagtggc gcatcacgtg gtccatgtcc aggttgaagt cgtccacgag
     2221 gacgatcggg tcttcgtagt tcaagtagta gcccatgtag aggggaaggc cctgccgcca
     2281 gatgctgagg gtccccggga tatgcccgcc ggcgaagctc gtcggagacc ggatatcaac
     2341 gatctgcgct ttccggtcca ggagagagtg gagctcccgg gccgagtatg cccgcaggtg
     2401 cggcaggttg tggatcaggg gcgggccttc ccggttgagt tttcgcatca tctcaaaata
     2461 cgctggctcg tagagccgtt cgttcacctt gcgctcgacg aactcttcct tcgagaggga
     2521 gagcaactcg ttcgtcgcct tttcaaaccc gatgctcgtg tactcccggt cgctgatatt
     2581 cccgccgcag accgaaccgg cgccgtgggc ggggcagacg atcacaccgt cggggagcgg
     2641 gagaagtttc tcgtgcaggc tcgcatagag catctctgag gcctcccgcc gcaggtcctc
     2701 gccggcaaga tcggtcctcc cgacatcccc ggcaaagagc gcatcgccgg taaagaccat
     2761 ctgtggttcg tcggagacgg accggtcacg gagcaggagc gatatgctct ccacggtatg
     2821 gccgggtgtc tccaggacct caagctcgag tgggccgagc ccgaacgtct ccccctcccg
     2881 gacgggggta ccgaacccga actcctcacg tgccccgtgc aggatctccg ccccggtcgg
     2941 acgggcgagt tcacgggagc cgatgacgta atcttcgttc ttatgcgtct caaagatctt
     3001 cgtgatcctc atattctcgt gctcggcgat ggtcgtgtag acctggcagt cgcgcctcgg
     3061 atcgatgacc gccgcctccc cgcccgcccc gatgatgtat gaattgtgag caaggatgtc
     3121 ggacttaatc ttctcaaaca gcatctgtct tcatctccgc gatgatgggg tccgtccccc
     3181 gttctgatgc gccggaatat gggagaacta tataaaaggt ttccgggaat acggagagat
     3241 ggacggacgt caccgtccgc cgcccggaca ttggtcttca cgggacccgg gcgggtgttc
     3301 acccggccgg gcggtcctga ccccgaggat gcttcccacc gccttgaacg catcctcaag
     3361 ttccgagaag ttcgggatcc ggcaaccccg cagaatgcgc aggccgctcc tgatgctgtc
     3421 gccgccaagc atgcagccca ccaccatctt ctccgtgttt cgggagagcc ggaggatctc
     3481 gtttgcaaca tgtgccggat cgacaagcgt ggtcggcacg gcgatcacga acgcgatgtc
     3541 ccagaagtcc tggtgccgga tcagcacgtc gaagagagcg gcgaaccggg tggcgtcggc
     3601 gtccccgagg atatccatgg ggtttgagtg gttccagaac ggcggcagga acgcattcaa
     3661 ctcgcggagc acgtcgtcgg ggaggtcgac catatcgacc ccatacgtct cggcgtagtc
     3721 ggaggcgagg acggcgaatc cccccgcact ggtgacggct atcgcgcgtt tcccctgcgg
     3781 gtacccctcg gacgccagga gttccgccag gttaaaggcg tcccgcaggc tccgggccac
     3841 tatcaccccc gcctgcctga acgccgcgac atagacatcg taactcccgg caagcgatcc
     3901 ggtatgggac gacgccgcgg ccttgccctt ccgggacgac ccggacttca ccgccaccac
     3961 cggctttctt ccggcaacct cgcgcataat ctgcgcaaac ccgcgcccat cctggatctc
     4021 ctcgacgtac agggtgaccg accgggtatt ttcgtcctgc tcggcgaacc ggaggtagtg
     4081 ctcgaatccg aggtccgcct ggttgccgac gctgatgacg gctgagaacc cgaactcttc
     4141 ggggaggctc cagtcgacga cggtggtgat gatggcgccg ctctgggaga tgaaggccac
     4201 atccccgcgt ctcggcgaga ccgggtcgaa cgtagcattg atccactggt gcggcatcat
     4261 gatcccgagg cagttcggcc cgacgatccg gataccgtgc ctgcgtgcga tcgccgtgac
     4321 ctcctcttcg aggaccgtgc cggcgccgcc gatctcgcgg aacccggcgg tgacgatgat
     4381 ggcaagccgg acgcctttct ccccgcactc ctccatcacc ccgggaacga ggcgggccgg
     4441 cacggcgacg accgcccagt cgaccgggcc cgggatatcc gtcacggagg agtaggcctt
     4501 tcgcccgaag agttctgtgt gggacgggtt gaccggataa aggttgccgg gaaaggagag
     4561 caggttccgg aggacagagt agcccacctt gttcgggctt gcggatgcgc cgataacggc
     4621 gatggattcc ggatagaaga gatccggggg tgcccggacg ctgaggcggg aggtggcgcc
     4681 cccggcggta tccccgacga tgatcctcgc atcgacaacg ctggctccct cctcgtacac
     4741 gatgaccggg ttcaggtcga actcccggat tcgcggatcc tcgacgaaac tctgtgccat
     4801 catcgcgatg acccggacga gcgcctcctc gtccttcggt ggctcccccc ggtatccgct
     4861 gatgagccgg tagccctcga tttcgcggat catctcgcgg atctcatcgt ttgtgacggg
     4921 cagcacccgg atggagacgt cctccaggag ttccaccagt ttccctccaa ggccgaacgt
     4981 gatcaccttc ccaaacgacg ggtcggtctt cccgccgatc agcacctcga gccctgcggg
     5041 catctgcttc tccacgatga cgccggtgac cgccgcttcc ggggcccggg cggcactgtt
     5101 ccgcatgatg gtccggaacg cttcttcggc gtcatccggc cccccgattc cggtgatgac
     5161 cccgccgacg tcgcttttat ggacgatctg cggagagata atcttcatga cgacagggta
     5221 gcctatccgt ccggcggcct cccgtgcatc gtcggcgctg gtggccaggt ggttgggcgg
     5281 cacgggtatt ccgcacgatt ccagcaggcg gtatccctca gattcggtta acatcctctc
     5341 cggcatgcat atccctcgct ctatgccggt cgtgccggtg ttgcatgacc ggtgtcctgt
     5401 ttcagggatt ggcccggtcg cccataatag ttatccatcc cgcgggagcg gacccggttt
     5461 ccatcaggga gtgtggcaga tgcggcagat gcggttttgg cccgtgaacc gccacggccg
     5521 aacatcggca cgggtaatac ttatttgcat ttagtcatac ctgataggtc ttaattcgat
     5581 aactataata aacagtgtta cgacaatttg tattagtatg acccagtctc gatacacagg
     5641 actcttcgcg gccgcagtgc ttgcggccct cgttctcgct atgctcgccg cagggtgcac
     5701 cggcaccgaa accggttcgg ccgtcgtcca ggatggtcag acgacctcga tcaccgacgg
     5761 gttcggtcgg accgtcaccg tcccgtcacc cccggagagc gtggtctgtt cgggctccgg
     5821 aggcctccgc taccttgtct acctgcaggg tcaggacctc gtcatcggcg tcgacagcat
     5881 cgagaaggaa gaacaggcga tggaaggccg cccgtatgcc ctcgcctatg ggtcgcagtt
     5941 caaggacctc cccctgatcg gtgagttcag gggcaaggac gaccccgaga agatcctcgg
     6001 catcggcccg caggtcatct tcaagaccgg ttccaccgga acagcatacg ggaccagtgc
     6061 cggcgatgca gataaactcg aggcaaagac cggcatcccc gtcgtggcat tcccctacgg
     6121 ctcgctcagg aacgacgccg agaaggccga gatgtacggc ggtctccgca cgatgggcca
     6181 gaccctcggg aagcaggacc gggccgaaga ggtgatcgcc tacatcgagg cgacgatagc
     6241 cgacctcgag caccggaccg gcgacatccc cgaggctgaa cagaaggcag tctacgtcgg
     6301 cggtatctcc tatgcaggcg cccacgggat catctccacc ga
//
LOCUS       DAJB01000179            5583 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65243, whole genome shotgun sequence.
ACCESSION   DAJB01000179 DAJB01000000
VERSION     DAJB01000179.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65243"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1113 a         1628 c         1616 g         1226 t
ORIGIN      
        1 tgccgttgat gatctcgtcg cggtagaccg ggtgggagag aaggcttttg agcgatcggg
       61 actccttctc ctttgagacc agatcgaacc cgagcgcaag cgcgaggagg cccccgtaag
      121 agaccagcgt ccggaacatc gaggagtaaa cgccttcgac cggcggtttt gcgggcatca
      181 tcccggccgg tccgtcatgt tggttgtcca tcgccgccag ttgctgggca tacctgtcca
      241 ggtctctctc gtagctcccg accccgctgt aggttcccgc cagggcgata cccaggaaca
      301 gggcgaggat caccaggaac cgccagcccg tgatctggtc ggtgaactct tttctggcca
      361 cagtgaagac ccgttccgct gtcatgtgac cctccgggaa accggtatcc ggaaggtctc
      421 cctgcccgtc gccggggttt ttgcacgttg atgtacctgc atctgcgcct tccccgttgc
      481 cgtcgatttc gctgtcatac ctttgccctc catgatcgtg cagaaccgta ccggttccgt
      541 ccatcaacat cgtcggctct tttcgggccc ggaaacgccg tccgggcatt gtcatccaga
      601 gactgggatg gaactctatt taccttttct ctcaaactcc tctacacgtc cgctgcccgg
      661 ggtcgcggta tccggtccgt ttccctcccc ggagcggacc tcccgaacgg gtgcgcccgt
      721 taccgttcgg gcgccatcga gaagccggaa cacagagaat cccgcgtcta ctccggtggt
      781 gtcaggggtc gaacgccgcc gggaggttca gccgcctctc gcggatggtc gatgtgacct
      841 cgaaccgacc ctctccgggt tcaaacgggg agcaatctac gctcagtgtt tcggagcaga
      901 ccccccggaa ttctcgggtg gtgctgtggg gcacccccgc ggggtcccag accgtcaggg
      961 tgaagatggc cgttctggtc ccgccgagtt catttccccg ggcgttgtcg catgcgacaa
     1021 ggaagacgct tcttcccgtc cccgtctcga tcgaaggggt cgcgtttgag agcgttgcgt
     1081 tgcttccgaa atcggcccag gctctgccga ggagatagac gccgtgggag tcgttatcct
     1141 gaaagaggat cttgccgccg gaggtggtgg cgttcacgac gatgtcctcc gtggcatacg
     1201 atacgagccc gtacccgagg aggccgacaa atcctatcgc cagcacgatg ctgcacaata
     1261 cttttccgct caataagatc acccaccgtt tcaaaaaata ggtgcatccc ctaggaatgc
     1321 acccccttca gacgtagtcg aggtccccgg tgcctatgtc cgttgctttc gttgtgtcga
     1381 gccaatcgtg cgtctcgcac cagagttcgt agtaggcatc actagtgccc tgcgaatcaa
     1441 aactcacctc gattgtcccg ctggttgccc agtccacctt tatttgttta gtgtgtgcca
     1501 taccttgagc atcgtagacc gtcagacggt agagtgctcc ctgcccgtcg ttgtcttcgt
     1561 cattggagtt gtagtacttc gctttgaagg tgttgtaccc ggccggaacg gttatgggtc
     1621 cgtacgtcgt caagcttgcc gtatcggcgt cgccgtctgt atacatgact cccagttctt
     1681 ctacggccag aattccgatg tcgccggtat cctcggttcc gacttcaatg ttctcctgac
     1741 tttgagcctg ggctgcggag accgcaccga cggaagccag tacaaccaat gccagcaccg
     1801 cccagaccgt gatagatccg tttcttgttt tcatcttcgt ttcctccatt gctacggcgc
     1861 cggaggggag agaacgtacc tataagtaga ggtaataaca accttctttt cgccctccgg
     1921 gcatgcgggg cgcatcatcc tgtcgtgaac tgcagatgtg tggccccgca actttatgtg
     1981 aatgcccgat tccggcaccc gcgctccttc ctcgacctca gggtatttac ctcttctctc
     2041 aaacctctct acacgtccgt tgccgtcttc ccgaaaacga aaggttgcat ccgtcctgtg
     2101 ccggcagggg gaacccggaa tcccccgtcc ggtcactgga tgtccatccg catgaaccgc
     2161 gtgtatgcgg cggcaaagaa gccgacggga tagaagatca gcgccaccac gctcgaccat
     2221 atctcgccga gcgtgtcttc aagggccgct cccgacgacg gggagttatg aatcctctgg
     2281 atcaggttgt tgatcgccct ccccggggaa aagaggtttg tggcgctcgt gatgacatcc
     2341 tgctgctcgt agtatgccgt aatctccgcc tggtagcctt ccgatgaggt atcccctccg
     2401 tatgcggcgg ggtcgggctc ctccatcatg agcgccgcgc cgatatgcgt ggcggtgaag
     2461 gggatcacga atgagaggag gatgaagacg accaccgcga ggagcagggc gctgccgctc
     2521 tcccggcaga aggtggaaaa ggcaagcgcg atggagaaga aggtcacgag gaagaggagg
     2581 gtgaccccgg cataggtcag gatcatccag aggtcgccgg gggagggtac gaccgagaag
     2641 acgaggaggc ccccgtagga gatcagcgtg agaaacatcg aggagtaaat cccctcgatc
     2701 ggcggtttgg agggcaccgt catccgctcc gactcgtcga accggtcgtc catcatcgcc
     2761 agctgctggg aatatctatc cagttccctc tcgtaactcc cgaccccgct gtacgtcccc
     2821 gccagggcga tggcgaggaa cagggcaaga acgaccaggt accgccagcc cgtgatctgg
     2881 tcggcaaact ctttccgggc tacggtgaag acccgttccg ccgtcatgcg accccctgga
     2941 gcgtccgtct ctcccgggag gcaagatcgg gctgaccttg gggctccgct ttggtgtccc
     3001 ctttctgaaa ctgggcccta actctattta cgttttctct cactcctctc tacacgtcgg
     3061 accccggagg gagccggaac agaccccggg ccagggtggt actacagcac aacgcaccgg
     3121 aggagagcgc agccctgttt cgaaaacttc aagggaaacc gaccacacat tactggttta
     3181 gattgtgcat ctcactggac cgaggactcc gattatgaga ctgctccttg ccgttatcgt
     3241 gggaataaca ctcatctatg cctgcatgca cttcttcatc ctgccggaga ggccggacgt
     3301 tactcgcatc gagaatgcaa cttacctgga atccccggca tacaacccgg ccgtcctgga
     3361 cctctgggat atcgtcgtcg agaggaccgg tgtcgagaac gagtcgacgg tgctgctgca
     3421 actagaggtc gacatcgctg gagacggagc gatccgggtt atatggctct tcttctacgg
     3481 ggtggaggac agcgaacagt atgcatacga ggcgtatgtg ggaccgggcg gaaccgtgtc
     3541 tgtcaaatcg cagaaattcg ctcttcccgt ggagggaaga catcccctcg ccattctcga
     3601 agaaatcgac gacattgctc ttgaagatct ctccttccgg gaccggggta tgacgttgct
     3661 cgcaagcgca aacgcatacg gggatcgata caacgagaca catgggaggc tctatgaagt
     3721 ctcgaatggg gtgttccgtc cggtgaaaga ggcaacgttc ggtccggatg cacgctggta
     3781 cactatcacg atcaccccgc accgtgacgc ggagcctccg tcgtccgggg cagaactccc
     3841 agactgcatc attgcgttca cgcggcagga tctcgaggcg gccgattccg tggtctacga
     3901 gtagtggggg tgagcgggcg ccaaaaccgg caacatcggg actcgttcat tatctccagc
     3961 gcgaatatct ccggatctac tcaacccgtc cctaagtgac gaagacctag ccggttgtgt
     4021 ccacgtccct gtccgcctgg atatattgta gaacatcctg ccgagggaag taacgcacca
     4081 ttcttccatc cttttcgctc cgtacggccc ctccctggat gagcagggcc atatgccagg
     4141 tcaccgatgc accggcgata ccgagcgtgg atgcgacttc tttccgcgtc attcccgggc
     4201 tccagccgag aaggtcgagt atcaacttgc gtgttccatt gagggtgtaa ctggtcttct
     4261 ctccctcgcg tgccgccctg ccgtttgcgt aatactgtag cagcccgtgg tcgtgctcgg
     4321 atactatctg ccccgtctca cagagggttg caaggtggta ccggagcgtc ccgacgttaa
     4381 tccccagcat atgggagagg gtcgagtggt tgatcccggg gttctcccgg atgcagagat
     4441 acgctgcacg acggatgtca tgttcgagag cgttcttccg ggagatcctc ctgtagccga
     4501 ggcagagcca tgccaacacc gatatgagaa tctgcaccgg gaggaagagc aggggggcga
     4561 tcatgaagac gaagtcaagc aggaccaact tcaggggcac attccagatg tatattgcgg
     4621 caggatcgtc tggtaccaac tccggaaact ccccctcgct cattatggtg ctggcagcgc
     4681 tccccgtggg gatgagcagc atgagagtga tgaagagcag ggtgctcctt gcggcagggg
     4741 ttcggttcgg cattgggaag atcaagtggc tggggatata aaaaagatgt gatttattct
     4801 gccgtggcct cgaaagtata agcctgcgta tcttgcacga cttccccata gacctgaaac
     4861 tgccacgttc cggactctgg cgaggagatc cacgtgcgga tctgggcatt cttgtaacca
     4921 tcgagatcga tgtcgtgata gtaggcataa gtgcttccac ttggcgagat gaccttcagt
     4981 gtcaacgact gctgggggat aaaccagttc aggttcacaa acagtgttga gcttccggga
     5041 ggcacgtatg cgctgtggcc gttcgtctcc ccctgctgta tagaataaaa ggcacaaata
     5101 cctgtgtcgt tttccaaggc attctctccc ggaccggtgt tcacaccaca ttcgctgctc
     5161 ccggcaaaag ccgacggcac catcaggacc agtgcaagca ccagtgtaaa gatacggttg
     5221 tatgtcatca taccacggga gaacttctct aacaacttcc cttaagtttt ctgtcgatcc
     5281 tctctacacg ttctccggtt gagggcaggc cggccgcccg ggaatcggtg tcgagcaatg
     5341 gtggaggggg cctgttgctc gaggtgagcg ctggtgccgg gttctcctgc aaaataggcg
     5401 tctgtgacgt ggatacggta tccggtatcg atttttcctt tgccgcgtgg gtttattatc
     5461 ggtcggcgca gtggaagttt ccggtcgact gcgggatgtt ttgggcagca aaatggatcg
     5521 aacagcctca acgtgtagag aggatcgaca gaaatcgttt ataacagaac gttgatttct
     5581 cac
//
LOCUS       DAJB01000180           15220 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_56438, whole genome shotgun sequence.
ACCESSION   DAJB01000180 DAJB01000000
VERSION     DAJB01000180.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15220)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15220)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15220
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_56438"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2830 a         4854 c         4793 g         2741 t
ORIGIN      
        1 ggcagtgcgt ggcgataccc ccacgaaatc cgtacacggg atatagcagg tagcaagcac
       61 cacccggccc cacatgccga taacgtcccg ggaaacaacc ctatgttatg agcaagggcg
      121 cggcgaagcc gccggatgga gtgggtccag ccaaaaaaaa gagactacgc cgccccccca
      181 cccgcacgga gcgggcagac gcccctgagt gcgtagagca gccccccgta gccgaggctg
      241 accgcgatga cctggacgat cgccccgagc agcggaatga agaggaggat ctgcaggatc
      301 acgaacccca ggacaaatat ccacgcattc cccggtctcc atccagcccg ggaggcaata
      361 acgtccccga gggcgtatgc gacaaagagc gacgagagca tcagggcaac gatgaagagc
      421 agcccgccga cgagcgcgag agggagaccg atgatcgtga ctgcgacgat gaggaggatg
      481 atcgccgata cgacgatcgc aacgaacccg aggaccgtga ggacgaccgg actcttctcg
      541 acctgccgga ccaccgccgc gaacgggccg ggaaataccc tgatgaggag gagcccgagg
      601 atcaggaatc cgatggcggc gagcaccgag aagaggcccg ggaatggtga ttccggctcc
      661 tcctcctcaa acgtcacgtt gccgaccgtg ccggtgttgt tgaaggtctc gcccgatacc
      721 gtcaggttac gcaggaccga accggcgttc gtgacctgcc ccgcgctgat gaatgcgtca
      781 cgctcgatga cggcatcctc cccgatccgg accgtgcccc cggttatgag ggcgttcgtt
      841 gcgttcccgt tcagttcgat ctcgcccccg gcggcgagca ccttcccgcc gacgtcgccg
      901 ttgatgatca gggtgcctcc cgcggcgatg atgtcgccgg cgaccggcgc atccaccgtc
      961 accatgcccc cggcgaccgt caggctgtcg acgggggcgc tgatggtcac ggacccgccc
     1021 gaggcgacca cgtcgtcggc gatgggagtg tcgatcaccg gctggtcccc gccaaggaag
     1081 gtgagcgcct gggctccgga cgggatgagc agcagaccga ggatgaacac tgccagatgc
     1141 ttcatgcggc agggatagat cgctctccag aaaaaagttc gtgcggaggg agagaaacat
     1201 tcctcgcaac cgtcaaggag gcttactgcc cgggttcgtc ccggcggtgc aggacccgtg
     1261 aggcggtgtg agagacctcc ctcccggcat aaaaatcgta gcggacctct tgcccgctac
     1321 accagcttcg caaggcggct cttgagctcg gcgatcgccc cgtccttccc ggggatatcc
     1381 gactcctgga ttgaacgaat gttctcctcg accgatgtgg cgagccactc cttcgggacg
     1441 ccggatgccg ggaagagttc ctgccactca tccgaaccga acggaatgac cttgaccagt
     1501 ttcccgagga cattccaatc gacgacccgg tagtcctctc cggtgctggt atccgcgacc
     1561 tgtatcgatc tgtcctcgtt gcactcgatg acccggccgt ggatgacgta gacactcacc
     1621 tcgtccttgt cggtgaaggt gatctggacc acatcccccg gttgcgggct ctcgggcacc
     1681 atgtagaggg cgatatcccc cggatacatc aggtccggtt cgtaccggtc tgcaataccg
     1741 atgtacccgg tgtcggagac atagagccgg aggtattcct ggtagaactt ccggacgatc
     1801 cagtaaactg ctccgggatg tgtctccgcc aatttcagga ggagattgtg gatggcgctc
     1861 gggatgctct ccggccgact cagcacctca cgtgccagcc tctcatacgc ttctacaacc
     1921 ccggcctctg tctgcgttgc ctcttccata tcaataaccc cgccgcatag taccggtggt
     1981 aggataaaag tgttcctccg gagatttcct ctgcatcgtc cgggtcaatc ccggcatcca
     2041 tccccgtacc ggcccgcgag aaggagagac tgccaatccc cgccaccggg tgaggggggg
     2101 aacgccggtg gcggagagaa ctataatgct tatattcgac cgtgaccata cagtaaagca
     2161 gaggatattg atatgggtgg cataaagaat tctacgtatc ttgatgtcca gaaggccctt
     2221 gcccggcgtt tctcttcccg ggacgggtgg cagtttgcat ggtcccccac ctacggcagt
     2281 gtgcagccgg aatgtatgct ctcccgccgg gtcgccggca ggacagagcg ggtggtcgtg
     2341 aacgtgaaga tggcctccga agtcctgatg gataccgtcg aagaactcca gagccagtgc
     2401 cggacacttg ctgcaggcaa cgtcaacgtc gataaggcgg tcctggtcgt ccccggcggg
     2461 gcgaacgtct cccgggtccc cgaagggatt gaaatcctgg agatggacag gtggcagatc
     2521 gtcggcggcc ggatcacctg gtcaaagaat ctcgagcgcg gtgcgttcat cgaggaagaa
     2581 cgcgcgaaaa ggggcctggc gtaaagcgcc ctgcccctca aactcttctt acaacccaaa
     2641 atcaaagagc gtcgtcctcg aggggtcgac gtcgtgccag tcccacccga gcggctcgat
     2701 gatccttgag agaggaccct tgagtgtctt ttcaagcatc gtctcccagt cgacgacgaa
     2761 ctcgggcggc acctgatcgg catactcgaa gcagaccacg tctgtgcgcg ggtacttcgc
     2821 cgtgacggtc ttgatgtaga cccgcttggg tttgctgccg cgcttgaagt cggtcccgag
     2881 atgttcgttc gagtacttgg cgccgcggat ctgggcgtcg tcgttctcgt aactatccag
     2941 gttcttcccg attccgccgg gaataccgac ctcgtcgagg ggatactccc cgcggcggta
     3001 cttcctgatg acggcgcgca ggtacgcctg cacgtcagtg aacgcgtccc cgcggaggat
     3061 catctcgatg acggcgcgct gcacttcccg cgtgatctgc ggtgaatcgc tccgccggat
     3121 ctcgaacccg acgacatcga tctcgtcgac gtccttgcct tccttccaga ccaggtgccc
     3181 ggcgtaacgt ttcttcttac cggcctggaa gaagcgctgg tagaccttct cgaacttgat
     3241 ggagaagtag tgcgtatcgg cgttcagttc gttcctggcg aaatcactgt aactcgcgtt
     3301 taaccgggcc tcgatagccc tcgcccgcgc gatggtctcc tcgaggttcc ccggcgggac
     3361 ctgcaccatg caggagtcgg tgtcgccgta gaggaccgtg tatccaagac cggtgatgat
     3421 gtcgcgggtg tgcctgatga tcgcccggcc taccgaggtg acggccgacc cgatctcgcg
     3481 gtcgtagagc ctgaaccggg tgtaacccga gaccccgtag taggagttca tgatcacctt
     3541 caggacgttc tgctggaggt cgtagaggac gtattccgcc gacccaaagg ggtactggtt
     3601 gcgcaggcgt ttgcgctcgt cccgctcccc aagaagttcc gagaggatgc tccttgttaa
     3661 cccgtccggc tcccgggaga atcggacgcc gtttggcgcc cgcagttcgc cgtccgggtt
     3721 cttcgtctcg ggggaggcgt tgatcgtcat catcgccatc gggtagagcg attttaagtc
     3781 gaggaccacc acgttctccc ggaggccggt ggcgggctca aagacggtcg ccccctcgaa
     3841 ctcgtccccg gcggcgagcc ccttcgaggg gaggacgaat gtccccgacg ctttccgcag
     3901 gacgtagatg tcgatgacgt ttgaggagtt cagcgtccgg tcgagcgggc acccgacata
     3961 ccgggagatc tcccggtaaa aatcgatgat attgtctttc ctgtcgatcc cgacacagag
     4021 ttcgacgtca cggcagttgt attccacgag ccgtgccggg tcggattgcc agagcgccgt
     4081 gatcgtcccg gtgtagcgga ccttcgtgat cccgagttcc tccccgccga tcgcatcgag
     4141 ccggtaagac tccttctggg cctggtgcat cttccggtag gcggaaagaa ggtcgaagac
     4201 cgccctcccc cggacggcgt tccgctcggt ctgccccggc aggcgtgcga ggtcctccgg
     4261 cctgatgccg agcgtcgcca tccgtttcag gacgtagggg atatcgaact ccacgaagtt
     4321 ccagcccgag aggatgtcgg ggtcgcgctc cctgacatag gtgacgagcc cccggagcat
     4381 cgccacctca tccgggtacc ggatgacccg gtgctgctcg tggatacgaa gtccggggtc
     4441 gtcgacgggc gcatcgcccg gcaccccgcc gggctgccag aggagggtcg tatagtcgcc
     4501 gtcaaacgag tcccagcagg tgacgcagat gaccggatcc cgctcgggct cggggaaccc
     4561 ctgctcgtcc acgcactcga tatccatgat acaggtccgg gcgggagcct tgatatcgac
     4621 cggcacgagt tcgcggtagt cgatcacgga gacgccggcg ggaagttcca cccctgcgga
     4681 gaggccgcag tctatcatga accgggtggc gaacgggata tcggcctcat agtgctgcga
     4741 aaacatgtcg cggacgttcc ggacgtcgct cgggcgccgg gtgtagaggc gccggagcgg
     4801 ctcgccgcgg atggagcggt aggttgtccc gggctccagg tcgacctcct gcggaaggga
     4861 tcgcccctcc accagaccgg cgggcacata gaaatagggc cgaaaaccgg tcacgtcgat
     4921 cttgaccgcc ctcccggagg tgtcccggcc gaagatgtgg atgaccggga tctcaccgcc
     4981 gacgctgtac tccacctggt gaatgccgac ccggaccttc ccgaagtcgt ccagggtagc
     5041 cggggtgctc atatgggact ccactgccag atcagccatc gaatctcctg cagaggctct
     5101 gcacgaacgc tcccgcctct gccatctttg cagattccca ggcatcgagg tcccactcct
     5161 cgatgccgag gatgccctcg cggccgatcc gggcaggaac accgagcgag cagccggaga
     5221 gaccgtattc gccgtcaagc acgcacgagc aggagagcac ctcccgccgg tcctcaagga
     5281 ccgcgcggat cagcatggcg atgtggtatg cagggccaaa gaccgtgccg cccttgccgc
     5341 ggatcacttc catgctcgcc ccctgcattc gggagaggat cgcttcccgc tcatcgaggc
     5401 cgagattggc gcctgccttc gagaagagcg gcacctggtg ctcgccgtgc tcgccgagca
     5461 cccaggcggg ccccgagacc cccgcctcgc tgaggtaacg ggcaaagcgg gtgctgtcga
     5521 gctggccgcc aaagccgatg caccgttgcc ggtcgacacc catcatcttc cagaggccgt
     5581 agttgttcgc gtccatgggg ttcgtgacgg tgatgacgac cccggagaac ccctgcagaa
     5641 gctcgctgca gcgcttcgcc accgggatat tggcatcgag gaggtcggcg cgggtcttga
     5701 tatcgggcgt cctcggggtg cctgccgcaa aaacgaagat gtcggcgtgc cgcatcgccg
     5761 ccgtatcggt ggagaccgtg acgtcgatgc ccgtgtgctg caggtcgagc acctgtgccc
     5821 ggaagatggg ctcgtatgcg tcatggagga tgatctcatc gacgaggcca agcgtggcgg
     5881 agagaaacgc cgtctcgccc cccacctttc ccacacctaa gattgcgagc gaggtcattg
     5941 tatcggtcct atatgtggat aacagtaaaa gactgtgcgc gccaaagagc cctgctgcat
     6001 ggagaataac gatccggcag agaaaagggg cactttaccg tcttccgttc cggtatccgg
     6061 gctccggaga acggagcaca tatttcttct ccagtacact tggcgcgagg gtataataat
     6121 acctcactcc cacctgtttt caatggttgc gctttatcct ggccctatcg aagtcggcgg
     6181 tatccggttg aagaaccacc tcatgctggc ggccggcatc ctcgggacta ccggggcgtc
     6241 gctcgcccgt ctcctgcaga acggtgcagg cggtgtggtc acgaaatcca tcggcccgag
     6301 cccgaaagag ggccatcccg gaccctgcct gatcgtcgtc gacggtggca tcatcaacgc
     6361 catgggcctg ccgaacccct cggccgcgtt cgtggacgaa ctggcagccc tgcagggcga
     6421 gccggtggtc gtcagcatct tcgggggaac ctccgaagag ttccggacgg tcgccgggtg
     6481 gttcgccggc acggcgtccg ggctcgagct gaacctctcc tgcccccacg cggagggcta
     6541 cggggcagca atcgggagcg atcctgccct cgtggaagag tgcacacgag cagtaacttc
     6601 cctcggggtc ccgacctggg tgaaactcac cccgaacgtc gccgatatcg gtgagatcgg
     6661 ggcggccgcg gaacgcggcg gggctgatgc gatcgttgcc gtcaacacgt tgaaggcgat
     6721 gcggatctcg acggcgctcc gccggccggt gctcgggaac cgtttcggcg ggctctccgg
     6781 gaaggccatc ttccccgtcg cggtccggtg cgtctacgat ctctacgagg cgtgctccat
     6841 cccggtcatc gggtgcggcg gagtctccac cgcagataac gtcgtcgaga tgatgatggc
     6901 gggggcaagc gctgtcgaga tcggaagcgc ggtcatcgac gatattgata tcttcgccgc
     6961 gatcgcgaaa gacctctaca gccacgacgg catcgacgca cgcgagatcg tggggtgcgc
     7021 tcatgcatga gcagatgccc acgggggttg cgataaaaga gatcgtagag gagacgccgt
     7081 cgatcaggac gttcgtcttc gaccgtgaga tcgccgcacg gccgggacag ttcgtgatgg
     7141 tctgggtgcc gggcgtggac gagatcccga tggccctctc ctccccgtcc tcgatcacgg
     7201 tcctgaaagt aggggatgcg accgccgccc tctttgcgat gcacgagggc gacctgatcg
     7261 gggtccgggg cccgtacggg aacgggttta ccgtctcagg aaggacgctt gccatcggcg
     7321 gcggcgtcgg cgcctccccg ctgctgccgc tcatcacggc cgggcaggtc gagaccttcc
     7381 tcctcggtgc ccggacagcc tccgagctcc tcttcgccga ccggatacgg gaatcggcga
     7441 cgctgatggt cgcgaccgac gacggcaccg ccggtcacca cggcttcgta acccggctca
     7501 tgtcaagagt agacctcgcc gacttcgacc acatctgcgt ctgcgggccc gaggtcatga
     7561 tggcggcggt gctcgccgtc ctcgaccggg aagggtgtgc ggagcggggg cagttctccc
     7621 ttcaccgcta catgaaatgc ggagtcggcc tctgcggatc atgctgcacc gacccgcacg
     7681 gcctgcgggt ctgcagagac gggcccgtct tcaccggcga catcctgctc gggagcgagt
     7741 tcggacacta cgcccgcgac gcgagcggga gcagacacag ggtatagtcc gccaaaaaga
     7801 ggatcaggcg aggaagtcct tctggggctc gaagagaagg gagatgatgt tctccaccca
     7861 cccgagcacc tgcgaggtga ttggaacctc ctcggcccgg gcgttcactt ccaccgcagg
     7921 atccgcgcta tcatctgggg cagacgggag agcatcgctc gccgtgctgt ttgggggttt
     7981 ggtatcgtct atcacagcct ccgtcggatc cctgctctcg atcagggccg cgttgtccgt
     8041 gaagaggtgg atggaccgat cgccatcgcc gcccccgacg acgaggaact ccccgtcagg
     8101 ggtcacggca acagactcca cacgaccgtc gagggtgatc ttctgaagat cggcacctgt
     8161 tttgtcgagg atatagactg tcctgccgga cgcggcgaag atctcgttcg cctcgtttga
     8221 gagagccgtc gcgagaacgg cttctcccgt gggatatttc cagaggtgct caccctcccc
     8281 cgtggagagg tagacgcacc ggtccaccga cccggctacc acgaaccgtc catcaggggt
     8341 gagagcaatg ccctccacgg atttgccggt cccataactc caggactctc cctccctgct
     8401 gttgaggtag taaaccccct ggttgtcgca gccgagcgcg atcctacggc cattctccga
     8461 tattgcaatg gcataaccat cggttcccat atcgtaatcc cagagttgct tcttgttccg
     8521 gtcgaagata aagaccgcac cgttgtcgca cgcggcagcg acatacatgc cgttggacga
     8581 gagcgctaca tcccggtaga caaaggtcgt tttctcggtc cagagctcgt tgccgtcccg
     8641 gtcaaagaga tagagtttgt ctgcggcgac gccgatgtac gatccctcgg gcgatattcc
     8701 gacgccgttc acccgggacc cgacaacggt cttccagaga acagttccct gctggtccag
     8761 gagatatacc accgagcccg caccggcaac gatcgtggat ccatcatgcg tgaccgccac
     8821 ggaactgata gcatcgtttg cccccagttg ccagagggga gcataccccc cctcatcagc
     8881 ggaggcggac ccgcagagag cagcgatgag gcagcagaac agaagaagcg gcagagagat
     8941 ccggccggat cgagagctat ggatcctgcg accggaatgc gttgcctgcc gaattcctga
     9001 atcgatccgg cttctggtag aactggatga agtcacttcc cccgatgaca tcaggccctg
     9061 acttctcgga gattatatat atagtttatg gggacgttac ccgccccgtt ctccggggcg
     9121 gttcgccagc tcccggccaa ccccgatcag gggctgccgg ggtttcggga atactttgca
     9181 taacgtttaa aggattcggg gaggcccgga ttccgggaac tcatcggccc gggatgcaaa
     9241 gagggtatag tgggtcggga gacaacatct gtcgattcga tgccggtaag acgttaacgg
     9301 tccgcttacg acggctcctg cccgggcccg cgcaggttgt cgcggccctg ccctgccgac
     9361 gggtcgatcg acttcaggtc ctccaggttg ttgatgttcg taaacgtcag caggtccggg
     9421 tcgatctccc ggatcttctc cacgcacacg taccgggcct caaggctcct caccatcgag
     9481 cggagcgaga gcgactcgtg ctcctccagg taaccgagca gtgcgctcgt ccggtagaca
     9541 gcgtggagcg gctcgagcat atcggcgttc cagcagggga tagcggcatc gtacccgaca
     9601 gcggcgtcaa agagcagccg gatcactccc ggatggatgc agggcatatc acaggcggca
     9661 acgaagacgt actcgccatg tacctcgagc gcccccgcgt gcaggccgcc gatgggcccg
     9721 agatccctcc ggacgtccgg tatgcacctg acgtccccga ggtgaccgaa ccgttcgcac
     9781 tgcccggggt cccgtgcgac caccacaatc tcgtcggcca cctcccggag ggtatcgatg
     9841 aggcggtcga tgaaggtctt gccccggaac gtaaagaaat acttctcccg cccgccggca
     9901 cgccgtgcct cgccgccaac gaggacaatc gcagaccgca tactatctct ccactgtcag
     9961 agccgctccc ggaagcggat cagttcggca atgcaggcat tatagggcgc gtcgataccg
    10021 tgcttccggg cgagcgctgc cacagcaccg ttcatgaagt cgatctcggt ctttcgcccc
    10081 agggcaaggt cctgcagcat cgatgaatga tgggcggccg tcgccggcaa ctgctcggtc
    10141 cggagatact ccaggtacgc cccgggggtc ttccagggga gggtgacgcc ctcggcctcc
    10201 accacctgcc aggcctcccg gacgatcgcg gtgacgatcg cccaggcgtg cggatcggtg
    10261 agcgcgccgt agggggcgtt cgcgatggcg ccgagcgggt ttaatgcaca gttgtagagg
    10321 gtcttactcc agatatcggc cctgatctcg ggggtcgcct ccgcccggat gccggcagcc
    10381 cggatgaggt ccgcgagaga ctcgaccgca tcgtccgtcc cggacggaaa ccgccccagc
    10441 ttcatgggcg ccgcctccac cgagacgtgg accgatgcat cgccccgcca ctcgaacccg
    10501 gtgatgatca tggcgccgat gacccggtcg gtaaaccgct cgatgatctc ctcgtttccg
    10561 atcccgttct ggaggctcgc cacctcatgc cccctgatag cgtcggcgaa ctggcggcag
    10621 acagactccg tgtccgtaga ctttgcggtg atgatgtagt agtcggcgtc ccgccacacg
    10681 tcggggaggt cctcggagca gctgaagcgg tatatgccct ctccccatat accggtcatc
    10741 agaaaccccc gctctcgcac cgcgtccaca tgccgctttc gggcaacggc gtggacgtcc
    10801 gcgacacggg acaattttgc ggcaacggtc aggccgaccg ctcccgcacc caggacggcg
    10861 atcttcattt cggagtacta tattgcggag ttacctgtta acttctccca tttttcaggc
    10921 gtcgcagacc cttccgcggc gctccatggc aagcagcgcc tccttgatcg tcacgtccgg
    10981 ggagaacccg cccatgccgg tcttcgcgac gacccggtgg cagggcacca cgagcggggt
    11041 cgggttccgg gccatcgccg atccgaccgc ccggggtgcg gtcccgaccc tccgggcaac
    11101 ctcgccgtag gtgacggtct ccccgcaggg aaccgcacgg accgcacggt agatcccggc
    11161 agagaccgat tccccctcgg tcgcgatact ccggagggag gcgaggtctg ccggccggcc
    11221 ggcacagtag cggaggagct cctccgggac cggcccggcg agcgcctctc gcgcgaaccg
    11281 cacccggtag acgagatcgt cctgccatgc gacgtggaca tgccagaggc cgaactgaca
    11341 cgacccggtc acgatcgcca tttgccagcc tccttccgga actcttcgac ggtgcacgtc
    11401 gtcatctcct tctgcatcca cggcggcagc ccggcatgaa cccgggattc caggaaccgc
    11461 cggtccccga ggacgagcat cccccggtcc tcgggcgtcc ggagcaccct ccctaacgcc
    11521 tgcaaggcgc ggttgatcgc cgggagggtg tagctgatga actctccctc ctcaccgaac
    11581 ttcatccgga agtagtcgat caccatcttc cggacacggt tgaacggggc gagcgggagg
    11641 ccgatgacga gcgctcccgc aagcatctcg ccccggtagt ccagaccctc gctccacttg
    11701 ccgccacata ccgcaaagag aacgcctgag cggcccgttc ccgggagggc catgaactcc
    11761 cggagcatcg tggcggcagc ggcagtgtcc ttggactcga cgtagatctg cttctttcgt
    11821 atccgcggag tgcaccgttc tgcgaaagtg ttcaagaggt cgtaggaggg gaagtagacc
    11881 gccagattcc cgggaagggc ggcaaacgtg gtgatgtact cctcggtccg cgcaaggttc
    11941 tgggggtccc ggcgcatcga gtaggtggtg gtgatgtcgt ttgcgcagaa gatgcggcgg
    12001 ttctcggtgg ggaaggagtt cgggagcgat gtcgtggtga cgggaaggtc gccgaagtag
    12061 tagcggcggt aactctcgat gggggagagc gtcccggata tcaggacgca gcaggcatgt
    12121 gcccgggcga tatcctggag cttggtgctg gggtcgatgt tcctgacctc gagcgccacc
    12181 gtgccgtcat cctccttccg gtagaccgtc agatacgccg ggtcggccgc agaccggaag
    12241 atccggtaga agaactcggt cagccgctcg atcgcgctct cccggaactc gcctgcctgc
    12301 atgttcttct cgcgaagccc ctcgctgatc ttgaggaggt cgtccacgat ctcctccatg
    12361 ctccggtaga gcgtgccgga aaggatcatc cgctggaaga tgacagggtc gaaccagtcc
    12421 tcaacctcgt gcgagccctt gagcgcctcc atgaaccggg tgatctgcgg caggatctgg
    12481 gtgatggcat ccgcctgctg cgaccgccgc cgaccggaga gttcgtgccc ggcctgcacg
    12541 atgtcgcgct cctcgatcgt cacgctctcg atgttctgga cgacatcgcc acagttgtgg
    12601 gcctcgtcga tgagcagcag ggcgtcgtgc cccttgattc cgagcaactg gtagagctgc
    12661 tcccggatgg tgtcgtcgaa gaggtggtag aagttcacga gcaccacgtc ggcatcccgg
    12721 gcggcatgca tcatcgtgtc gtagggacag atgtcgccgc agaacccggc aagctggtcg
    12781 ggccagatca tctcggtgct cacgcgctcg gcccggacgc gcagggccgc cgacgggatc
    12841 atcttcagcc ccgcgtcctc gggctccacg aaggatttgc cgtggatgaa gtaagggcag
    12901 atgagcgggt gctccgggtc catcttccgg atctgctgct tgatctggcg gtcgtttgcc
    12961 gggacgagcg atcccttctg cgcccgctcc cgcatcagcg ccgtcgagaa ggccttgacg
    13021 ccctcgcacc gccggtagac gtccccctcg ccgccgagcg ggcacatgct ggacttgccg
    13081 ataaggtagg cgaacttcag tttgccgcgc ttcttccgga cgagctcgag ctcgcgcatg
    13141 aacgtggcga gctggctgat ggtccggacg gcgacgagca ctttccgtcc ccggctctct
    13201 gcgaggagcg cggagaccac gctcgacttg ccgctcccgg tcggcgcatc gatcatggcg
    13261 atgccgccgt cgcgtgcgat ggatgcagcc agatcaagca tctcccgctg gccggatcgg
    13321 tactcccggt acgggaacca gtcgtcgagg gtatccattg ctaataggtc gacggctgac
    13381 gtcaatgtag tttgggtgcc cggggcgaaa gtgaacgagg cagggaacgg gtcgggcgcc
    13441 ggtggaagcg aggcgagacc ggggatcccc tgaccattgc ccgggtgctg cctatggcgc
    13501 ttgcggcgtt catccccacc gggaaccccc atggcgcgca tatgctcccg cctctccgga
    13561 aaaagacgga ctaaaagttt ccagtacggc ctcgctggag acgcgaacca aaatatttat
    13621 ataacgaacc gctcaccgct caccggtgag actgtatgtc actcaaggct gaggtcatcg
    13681 acaagatatc ggctcttatc acggccgcat tcggcctggt cgccgccctt gcatggaacg
    13741 gcgccatcca ggaactcttc gnntcgtcgt gacgatcatc gcggtgatcg cggtcatcct
    13801 gatcggccgc gccgcaggga gggcgaaggc agaggatgag gcggcagcaa caaggtgagc
    13861 cccggaactc ccccttttcg gcaaatttat ccagacagaa acctacctga tcgctgatga
    13921 gccggcatga cgatcagcca gagggcaggg caggtgtgga cgagtcgccc ggccctgccc
    13981 gcgcgcgacc gatcaccgtc acggtcatcg agtacgacga gtcccgcttc aatgagcaga
    14041 cctgcacccg gcccggggac cttcgggcac tcatcctccg cccgacggtc acctggatca
    14101 atgccgacgg catccacgat accaggatgg tccaggcaat cggggacgct gtcgggatcc
    14161 acccgctgac cctggaggac atcgccaaca cgagccagcg accgaagatc gaggactacg
    14221 gcgattatct ctacgttgca atcaggatgc tctcccctga cagtgacggt gagttccaga
    14281 gcaagcaggt cagcctggtg ctcgggagag gttacgtcat atcgttccag gagcaaccgg
    14341 gcgatgcctt cgagcgcatc cgggagcgcc tgcgtgcagg agccgggcgg ctccggagcg
    14401 agggggccga ctacctcttc tatgccctgc tcgacgcgat tgtcgacggc tactttggcg
    14461 tgatcgaggt cttcggggag cgtatcgagg cggtcgagga ggaggtggtg gcagaccccg
    14521 accgggagac cctgcaggcg atctacgcct tgaagcggtc cctggtcgct ctccggcggt
    14581 cggtctggcc actccgcgac gtggtggcgg aactcgagcg gggcgactca cccctgatcc
    14641 gggaatcgac gctcgtctac ctccgggatg tctatgacca caccatagag gtcgccgaga
    14701 ccgtggagac ctaccgggat acgatgtccg ggctcctcga cgtctacctc tcgagccaga
    14761 gcagccggat gaacgagatc atgaaggtcc tcaccatcat cgccaccatc ttcatcccgc
    14821 tcaccttcat cgccgggctc tacggcatga acttcgcgta tatgcccgaa ctcaggcatc
    14881 cctggggata tcccgcggcc ctcaccttga tggcgatcat cgcgggggtg atgctcctct
    14941 acttcaagaa gaaggggtgg atctgatcgg gaggggcggc tgatcgccag cgccagaccc
    15001 ccgtaaccgg aataccatta tatcccggta agtcgaagcc cgcgcaaccc tgctgcgagc
    15061 ggaagcagac cgcgtggacc accggcggca ggatgtgggg catgcaggca gaccgagggc
    15121 acccggagga gcaggaagag aagcgcgcga cgctgaggac tgtcgccgtc atcaccgggt
    15181 tctacgggat gaatctcgtc aactaccccc ggttaaaacc
//
LOCUS       DAJB01000181           18025 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_14894, whole genome shotgun sequence.
ACCESSION   DAJB01000181 DAJB01000000
VERSION     DAJB01000181.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18025)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18025)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18025
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_14894"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3783 a         5556 c         5297 g         3387 t
ORIGIN      
        1 ttcatcccca ccctgaaggg ggggggtctt cccgctccgc ccccttcacc cccacaagat
       61 aaaccgcttt tttcggtaaa atccatcttt ggaacatcgt attcaaaatt gacaacattt
      121 aatgggtctg atgcggtaac gctaaatgtg gactatggca aaaaaatcat ctccgcagaa
      181 gaaagcaatc gagcaatacg accataaggg aacctgccgg ctgaataacc cgccggcagg
      241 tctcgtaagt gcggagaccg aacccgagta cgacacaaag acctactcgt atgaccctca
      301 cctcgacccg caactccagt gggcggggaa ggccgagcat acgtcgttcg aggtgccgac
      361 ggtctccctc cacgtccacg aacggatcga ctcgcggacg atcatcaggg cggcgaaggg
      421 cagcaatggc gaccgccggc aggcctcgct ctttgagttc tcgatagaga acccgccgct
      481 gcgggaggcg gtggagttct accggcacaa gcacaactgg agtaaccgcc tggtggccgg
      541 cgactccctg ctcgtcatga actccctgct ggagaaggag gggatggccg ggaaggtgca
      601 gatgatctac atcgacccgc cctatgggat caagtacggc tcgaactttc agcccttcgt
      661 gaacaggcgg gacgtgaagg acaggaggga tgaggatctc acgagcgagc cagagatgat
      721 caaggccttc agggacacat gggaactcgg gattcactcg tatcttgcgt atctccggga
      781 caggttgctg ctcgcccggg aactcctgac cgagggcggg agcgtgttcg tgcagatctc
      841 agatgagaac gtgcatcatg tacgggagat catggatgag gtgtttggct caaggaattt
      901 tgtttcaatc atctactttg ccaccactgg cggatttgct accacatcgc taagccgaat
      961 tggagactac cttatatggt acggcaaaga tcgaaatctc ctaaaatatc atcaactata
     1021 cgccgaaaaa ggattgcgcg aaggtggcgg ttggtctcac tcacgagctg aatttgagga
     1081 tggaaggcgt gagaacattg gtgtcgatga agtacgacgg ttattagatg aaggacgtaa
     1141 gattcgacca tattcattag atggacttac ttcacagggc acatcaaaag agagtagtga
     1201 accatttgaa tttcgggggc gtatttacaa tctaccccat aattcgcatt ggaagacaag
     1261 ctctgagggc atgaaacaat taattagagc agagcgaata gaggcttcca ctaatagtat
     1321 tcgctacgtt cgatttattg acgacttttc cgtcaagcct ctcaccaaca tctgggatga
     1381 tacaaatcga cccggatata ttgacgaaaa aaaatatgtt gtgcagacct caaccaaagt
     1441 catcgaacgc tgcctcctca tgaccaccga ccccggcgac ctcgtccttg accccacctg
     1501 cggctccggg accaccgcct atgtcgccga gaagtggggc cggcgctgga tcacctgcga
     1561 cacctcccgc gtcgccatca cccttgccaa acagcggctc atgaccgccg tcttcgacta
     1621 ctacgacctc gcccaccccg aggagggcgt cgggagcggg tttgtctata agactatccc
     1681 gcacatcacc ctcaaatcca tcgcgaacaa ccccgcgatc gacgacatct acgagaagca
     1741 ccggctgaag gtcgaggccg ccctcgctga catcaacgcc gccgcgaaga cctcctgcga
     1801 agagtgggac gtccccttcg atcccgaccc cgcctggccg gaagcggcag agaaggccca
     1861 taaaaagttc ctttctgtcc ggcagaagcg gcagaccgag atcgacgcga gcatccagaa
     1921 gaacgctccc caggagaccc tctacgacca gccgcaggcc gacaaaacaa aagtccgcgt
     1981 caccggcccc ttcaccgtcg aggccgtccc cgcacccacc gtcgaaccgc tcactggaga
     2041 cactcccgca gtcctcccgg ccgacgactc cgctgctcgc accggcgaat cccttcggca
     2101 ggacgaatgg cgcactgaac tcctcgccgc cggtatccgt ggcagaagtg gtcagcggat
     2161 cgccttcgcc cggctcgaaa ccctcggcgg cacccggtgg ctccacgccc gcggcgaaac
     2221 taaagatgac gccccgaaac aggtcacagt ctccttcggc cccgagcacg ccgccctcaa
     2281 cgcgagacag gtcgagatgg cgctcaaaga ggcgcaacgc ctcgtccccc gacccgagat
     2341 cctcgtcttc gccgccttcc agttcgaccc cgaagccgca aaagacatcg acgagaccaa
     2401 ctggcctggc gtcaccctcc tcaaacccaa actattccct cacttanncc ctaatcgcag
     2461 aatagtttgg gctcaaagcc cagatgaaca ccgacctcca gaccgacgac ctcaagaaga
     2521 aatgctcccg caacgagagt ttctggctca tcggccagcc cgacatcacc ctgaacctga
     2581 tcaccgaagg gcccgaggct ggcacgatcc tcctctccgt caacggtttc gactactacg
     2641 acacccgcaa aggcaccgtc gacggcggcg gccccgacaa gatcgccatg tggatgctcg
     2701 acaccgacta cgacggtcgc agcctcttcc cccggcaggt cttcttcccc atggccggca
     2761 aagacgatgg ctgggcaaaa ctcaaaaaga gcctcaaggc cgagatcgac gaagagaaga
     2821 tcgaggccta ccgcggcact gtctccctcc ccttcaagcc cggggagaag cggcgcatcg
     2881 ccgtcaagat cgtcgatgac cggggcatcg aatccatcat cgagcgggat ctggacaacc
     2941 tggaggcata actcatgccg cagaagacca tcgaccgcct catcattacc tcgccctaca
     3001 ccgaacctgc ctgccactgg cattacgacc gaaccacccg cacattcgat cttgcagaag
     3061 gccggcgcaa ggcgggctac atcgtcgcca cccggggctc cagcgccttc gacgatcctg
     3121 gtgtcttcta cgagatcccc ctcgtcaacc agatccggcc ccgcatcagg gcctggcagg
     3181 aggccggcta tcccggagcc accagcatca cccgccgcct cctcgactac tggcaggacc
     3241 gcgaagagcg gggaagcatc cccttcttct tctgccagct cgaggccatc gagaccctca
     3301 tctggctcac cgaggcgccc gccgcagaaa agaccgggat cgacatcacg ggcgacggtg
     3361 gcgagttcct ccgctactgc tgcaagatgg ccaccgggac cggcaagact gtcgtcatgg
     3421 gcatgctcat cgcctggcag gtcctcaaca agaccgccta ccccgccgac ccccggttct
     3481 ccaaaaacgt cctcgtcatc gcccccggcc tcaccgtcaa aagccgcctc tccgtcctcg
     3541 tcccctcctc cgagggcaac tactacgaca cgttcgccat cgtcccgccc ggccttgcgg
     3601 agagcctcag gcaggggaaa gtcctcgtcc agaactggca ctcgctcgcc tgggagagcg
     3661 aagagcagat ccagaaaagg aagagcgtcg acaaacgcgg tccaaagagc gacgaggcct
     3721 atgtccgtga ggtcctcggt gatctggccc ggcacaacaa cctcattgtc atcaacgatg
     3781 aggctcacca tgcctggcgg gttccggccg agtcgaaggt caaaggggtc gccaaagacg
     3841 agatcgatca ggccaccatc tggatccagg gtctcgaccg catccacaag gcaaggaaca
     3901 tcctctcctg cttcgacttc accgccacgc ccttcgcccc gagcgggaag aggagcgacg
     3961 aagaagccct cttcacctgg atcgtcagcg acttctcctt aaacgatgcc atcgagtctg
     4021 gcctcgtcaa gactccccgc gtcgtcatcc gtgacgacac gaacccgagg cccgacgact
     4081 ataggtcccg gttctaccac ctctacaccg atcccgatgt ctacgacgac ctcaaccgca
     4141 agggcgccga agaatccgaa cccctcccgg acctcctcaa caccgcctac tacttcctcg
     4201 gcctcgactg gcaggagaag aagaaggagt ggattgcggc cggctaccgg accccgccgg
     4261 tcatgataac cgtcgcgaac accaccgaga ctgcggcgag agtcgcctat gccttcaccc
     4321 acaaaaagat ccggatcgat gacctctgcg acagtgacag aatcctccac atcgactcaa
     4381 aggtactcaa agaggccgag accctcgacg aaccactgac catcacaaca ggcgaggatg
     4441 aaggggtgaa ccggccgctc accaaaaaag aaagggctct ccacctccgg cagcaggtcg
     4501 acaccgtcgg gaaacccggc gaaccgggcg cgaaggtcca gaacgtcatc tccgtcggca
     4561 tgctctccga gggctgggac gccaggacgg ttacgcacat catgggcctc cgggccttct
     4621 ccagccagct cctctgcgaa caggtcatcg gccgggggct ccggcgcacc agttacgatg
     4681 ttgacgagga gaccggcctc ttctcgcctg agtacgtcaa catcttcggc atccccttca
     4741 ccttcctccc ccacgagggg ggagacgacg caccgccgag gcaggagaaa ccccagaccc
     4801 ggatggagtc gctcccatcc cgccgggagt acgagagcac ctggccaaac gtgctccggg
     4861 tcgagcacac ctaccggcca cggctcaccc tcgatatcga gaccgccaaa tctcttgttc
     4921 tcgacgctgc ccagacaccg cagattgccg aactcgctcc ggtcatcgac ggcaaacccg
     4981 ctgtcgacca gatcaccacc atcgaccttg aggaactcgg ccggatcagg ctccagcgga
     5041 ttgtattcga gacagtacgg gatctcctcg accagatgcg cccggagtgg cggggtaccg
     5101 ccgccgacct cgtcgggcag ctggtcggcc tcgtcgaccg gttcctccgt tcggataagg
     5161 tcaggttcgc acccctcatc tttagcaccg atgacacgcg ccggcgggtc ctgctcatcc
     5221 tcaacatgaa caaggttgtc cagcacatct acgagcagat ccgcgagaca aataccgaag
     5281 ccatcgagcc ggtcctcgac ccggcacgcc ccattcgctc gaccgctgac atgccggtct
     5341 ggtacaccac caagccctgt ggagaaacca taaaatcgca gatcaacttc tgcgtttatg
     5401 atagcacatg ggaggccacc gaggccttcg cccttgaccg aaaccctgca gtgcaggcat
     5461 gggccaagaa cgaccatctt ggatttgaga tcttctatct ctaccagggc gtcgtacgaa
     5521 ggtatcgccc ggactacctg atccggcttg agaacggcga aaacctggtg ctggaaacga
     5581 aagggcagga cagtcagcag aacaaggcaa aacgggccgc ccttgctgag tgggttcgtg
     5641 ccgtcaccgg gcacggcggg ttcggtacct ggcactccga cgtctcgttc aacacggccg
     5701 acgtcgacgg gatcattgcg aaatacaccg agaaacagcc tgccagccag gtcgagagtt
     5761 tgaccgtatg accgggcgta acaatctgag taagagggct ggaacgggtc ttacttttcc
     5821 agtatatatt ccagattcat ccctcattca actgcctttt cgccgtgact cattcccatc
     5881 cagcaccatc acccccgccg acgtcgcccg ccacgaccgg atcgtcaccc tcgtggagca
     5941 gatgctcgac ctgaacaaac gcctcgcggc ggcgaaggct ccgcatgaga aggaggtgct
     6001 cgccgggatg atcggcacga ccgaccggca gatcggccgg ctggtgtatg agttgtacgg
     6061 gctgacggag gacgaggtgg cggttgtgga gggggctgcg tgaggtttta caacgatccg
     6121 ttgaagatca gcgctgaagt ctgggctgaa ctgctggtag acagcgacgt gacaaaggaa
     6181 tccgacttaa ttgtgttacg gctggtgtat gagagtgtaa accacgagat gcacgcatca
     6241 gagatagcac ctcatttgaa ctatcagatg catgcgccgg tgaacgcgaa aatagctcga
     6301 tttagcaaac gggttattga gaaaactgga gcccggcctc ctacgagaga aaacggaaca
     6361 gcccgatggt ggcatgttcc ctttttaggc tatgataaac gaaacggaac gtgtgcctgg
     6421 attatgcggc ctgaactggt ggtggcgtgt gaacaggttt tcggtcaaaa caactcagaa
     6481 atcgtttatc ctggagaggt gaacattgaa gataacaccg tcctatccga aggtgccgtg
     6541 agtcaggttt ttgtaaaccg ttacgaacgg agcaagcagg cacgaaccgc atgtatagaa
     6601 catcatggct gccgatgtgc cgtttgcggt tttgattttg agaaggtgta tgggccgata
     6661 gggcagaata aaatccacgt gcaccatctc atacctctat ctggaatccg acaggaatac
     6721 gagatcgacc ctatacgaga tctacgtccc gtatgcccga actgtcacct cataatccac
     6781 ggtaagagag agcctttcac aatcgacgag gtaagagaac aaatcaggtt caacagcggt
     6841 caataagtgc ttacatgaat tcccatttgt accgttgaca atcggtcggt ttgctcaggc
     6901 gctttcgata gccgaaattt aagcgatgcc tgaatacctg ggggaggata cttttacatt
     6961 gagagaaaag aactctctat gtcactctac gaccggtggc agggatcaga actctctgtt
     7021 ctggagaaag agctcatttt caatagagag aacaccggct tcgtccagta tgacacaaag
     7081 gacggaaaat acttttatct tcacccctcg ccgttcttga atagcccaaa gaaggagatc
     7141 ttctgtaccg agttgtgcga cccgccggcc gaacatacat tcgtcgaggt cgatatcgaa
     7201 tccgaaagag agtttccact caaggggtct caagaccaca tcaccgtgaa aaccatctgc
     7261 ggctggaaac ctttcgatcc gagcctccta gcagagcgca gaaaaattct ggactacgaa
     7321 gagatcgtgc attactttac cctgccgttc cagggtgagg aggatacgat cgaacagatt
     7381 gcaaaatgtt ccgctctcta ctccctctcg tctcccccga tagtcgatga ggtgggaggg
     7441 atcaacgccg ctatcctcgg gaaaaaaaca cagtggaata ccttcaaagg gtcaatgaag
     7501 gtcattcctc aggacttctt ccgttccaac tcacagtatt actactacat atccgaccag
     7561 gagaagtgca cggtcaaaag cggatgcgag gaggtaaacc gggcgatcta ccgccctaat
     7621 cagtatgtca tggatattcc gctcgttgtc gaggaagcgt cacctagccg actatcgaag
     7681 gattacagag aactcataaa cgaagaaaag ccgcttatta catcctacat tctcgatgct
     7741 ctcattatca aacccgaccc gatgaactcc gttgagaaga cgatcaccga cgcggtatat
     7801 acgctccgcg aagagtacaa acgaaccggg tactcgccct acaaacaaaa cctgggcgat
     7861 gcaatcccca agctgacctc atcgcttgcc cgacttcagc gagcctcgaa agcaactcaa
     7921 tccgatgtca aagatgtcgt tgaactctgg ctggatatgc actggaaagc gaacaaaatc
     7981 tactcctctc cactcaaggt ctcaaagcga tatgatctgc tcgacaccgc acggaagctg
     8041 tatgtggaac tccacgacat ctacggactc gagttctgga ttccgctcga cgaagcagtc
     8101 accagaacta cgcttcatcc agaggagttc atggtatctc ttgaatcact ggtcttcgcg
     8161 ggatacgcgt tgagaaaagc gggaactata aaaattctct agactccaga ccctcaagat
     8221 ctggcattcc ggagcgccac ctctcttcaa agataccggg caaaatcgtc aggatcaatc
     8281 ttcgccagtt tcaggacgtt gcggagcgtg ccgatcttca actcgtcgtg caatgggaca
     8341 actgtaccga ctttcccgcc gggcgtcgcc tttgacaggc gtacgtggct ccctgattgc
     8401 ccggtcacag aaaatccgta gtgtttcgag agtatcttta tcactgtctc gcccgagact
     8461 ggtttaagcc cgggcaccct cggccacctc gaacgtcgtg atgatcggtc ttctcccgtc
     8521 ttccagcggg aactcttcca gatagagctc cgtagcctcc ttcaggttgg ccactgcttc
     8581 ttctaccgtc ctgccctggc tgacggtgcc gacttccggg cactccgcta cgtacatatc
     8641 ctcttctctg tagagtacgg ccgtgaacgt cctcatgatc tcaccgatag aatagcatct
     8701 ctctccgggg tgataatact gaccgacgca cccgtgtgtt cctcccgtca acgttgaagg
     8761 gttcaaacgt gggttaaaac ccggactccg tcacccatac cgctctctca catctcccgc
     8821 ggttcgtatt gtggcatgac ctcgactaca cccggacttg ggctacccgg cccggtgtgt
     8881 cggggttatg cagtgcgggg cgataggttg tggatggggg gacacgacaa ccgactgaac
     8941 cggcttgacc tgcctcaaaa catcggcatc cggttaaaaa acgccctgta gccggtcttc
     9001 gtgtttctgc cccgtgtagg ccgcaccggg ggtcggcggg ttattccaac gaccccccca
     9061 gggcaaccgc ttgcccgccc gaaactaccc ccgcctctcc ccccggatca gcaccctcca
     9121 ccggtgcagc atccgccgga gccctgcggt cccgacgggc gccgggtacg ggagggggat
     9181 ggcggtcccg tccggggcga tggtgaggcc gagggcttcg gcgcattcgg tgcagatggt
     9241 ctctgcatcc ggatcggcct cgagcatggg gtggggaggc tcgaagtgca cccggctgcc
     9301 cgcgatgtcc gcgaacctct cgaagatcgc cgcctgacag gcgcgctcct cttcgacggc
     9361 ggccgaagcg tcgatgccgc agatgcggcc gacctcttcg aggaaccggc acgtccccgc
     9421 aaggccggcc gggaacgagt ccacgtaggg cgtggcaaac ctcttccgga ggtcttcgcc
     9481 gaccggccgg agcgccggtt ctctgaggat gttgacggtg gcggacccga ggcgtccgat
     9541 atcccgggta tcgagccccc gcacgaaccg gaggttgatg ccgatgccga gccgggaaag
     9601 aaggcgtgcg atctcggctg cgttctcgtc gacgccgtac tccaggttct tctcgccgac
     9661 caggtttgcc gagagcgtcg tctcggcaag aggccgggcg agggacgctg ccgaagcgag
     9721 ggcgtttctg atgccggtct caaacacccc gccgaggaac cccgcggtcg gcacggcgat
     9781 caccgggacg ccccgggcct tcgcacagac cgccgcggtg tcgtccccga tcgtctcgac
     9841 gatgcaggtc gagaggacga agacggaagc aggggagagc gagagcgccc gtgcgatggt
     9901 cttctccagc gcctcttcgc ccccgaagat gatatcgttc tccgttaagc gggtggagag
     9961 gaggcggggg gcttcgagcc ggtcgttcga gagcagtgtg gcgtggagga gggagaagtt
    10021 atggtgcgta caccctgccg gaccgtggac gatgcagaca gcgtcccgta cctctgttag
    10081 caccgagagc gctccggtca gggtgcaccc ctcaaacctg gacgaattcg agggcgaggg
    10141 attcgagttc atccatctcg aggggtgtgg ggatactcgt cgtttcgttc ccatagatcg
    10201 tgcgtgcaag ttcctggtag accgctgcct ggtctgattc aggggcatac tcgacgaccg
    10261 tctgcttgtg cagctccgca acctgcacgt cccgggaccg tgggacgtag gcgatgagcc
    10321 gggagttgat ccggcgggcg aactcctcca ccagttcccg ttcgccttcg atgttctttg
    10381 cgttgcagat cacgcctccg agcgagcatt tgctccgcac ccggtgcgag aggcgggcga
    10441 tagccttagc aatgttgtta gccgcgtaga tggacatcag ttcgccggag gtgaccaggt
    10501 agacctcctg cgcgtatcct tcccgcatcg gcatcgcaaa cccgccgcag acgacgtcac
    10561 cgaggacgtc gtagacgatc acgtctcctt tgagggcctc caggcgttcg aggagctgga
    10621 acgttgcgat gatgccccgt ccggcgcacc cgatccccgg ttcggggccg ccggcctcca
    10681 cgcagcgcac cccccggaac cccttgtaga ccacgtcatc gacggcgatc atctcgtctc
    10741 cgcgcttccg gatgaggtcg agcaccgtcg ggatccaggt cccgtgcatc agcatccggg
    10801 tgctgtcgtg cttcggatcg cagccgatct gcaggatatc gagcccttcc cctgccagtg
    10861 ctgccgatag gttcgctgca gtggtggatt tcccgattcc ccccttcccg tagagggcga
    10921 tctgtttcat tgtttctact atgcgcttgc atgggtaatt aggtgtcgga tggtgctcgc
    10981 acacttaatc tatatctact ttgttgtaat ctgggatatg ttccatggaa gatcttttac
    11041 ggaaaaatag ggcattcctc tgtattttga taatctgata tttataattg gatttttaga
    11101 gttagaaaag tatataatta tccgattgta actcttgtta agcagaggga aaaccatgga
    11161 cccacgattt cgatcacggt tgattgcggc gatcattctt ctgatcgtcg tctgttcggc
    11221 cttctcagtg agcccggtcg ccggtttccg tctggaaaac agggatggga gcggcactga
    11281 agcggcactt gcagaggccc tggtgctgca gcagagcacc aaaatacgag aagagtttat
    11341 ggagaacctc acggtctaca tcgacagcga gaatgcggtg ttccggcagc agaacagcag
    11401 cgcgagtggc ctgtatgtcc ctggagagaa cgccatctac atccgctcgg acaggaaccc
    11461 gtcgcaggcc gacgaggtct ttgccgagca ggtcgggtac cgggtctacc gcaccatggg
    11521 atttgaggag agcgcggtct ttgcggcgct cgccgcgaac tccggaccct acctggccgg
    11581 tatcagcgca tcctccggtg aggagaggga ggcggcgctc tttgccgatg ccttcatgct
    11641 ctaccacacc accccggcac tcttaaagaa ggatgccccc ggcgtctacg cctacatgga
    11701 cctgctcgca aagaacggcg gggatgcggt ggcggtggac gacctgtacg cccggcaccc
    11761 gcaggcgtaa ccgatatatc ctttttttgc ggttcactcg aaccgcttga ggacacccat
    11821 aacgagcatg atcaccggaa tgatctccag acgtcctatc cacatgatca gaatgaagag
    11881 gaccttcgcc ggttcggcca tgtcgggcga gacgaatccc gtggagatgc cgttgttgca
    11941 catggcggag acgacctcga agatcacgtt gcttgagtcg aaggacgtcg gctgcaggtg
    12001 catcaccagt atcgtggcga cgaagatggt caggaagtag agcatgatga tcagcatgtt
    12061 ccgcgagacc tcgaggtcgg cgacgttctt cgggatcacc ctgccgtcat acttgaaggg
    12121 cacgagcacc ttcccggaga cgagcatccg ccggaaccac cagagcaggc tctgaaaccc
    12181 gaggactacc cgggagagct tgataccgcc ggcggtactc cccgacgccc cgccgatcac
    12241 gatcagcatc gagaggaaga ggaccgtcac gctcgcccac tcgttcgggg cggccacctg
    12301 gaacccggtg ctggtgatcg ccgcagtcga catgaagagt gcctggcgga tggcggtggg
    12361 agcgtcggtt gcgctgagcg tgatgaggtc ccacgttatc acgacgatcc cgagcgcgac
    12421 gagcataaag aggaggcggg cctgctggtc ctcgatgaac cgcacgccct tcttgcggta
    12481 gaggaggtag tagagtttga acggcagggc gccggcgatc atgaccggga cgatgaggac
    12541 ctcgagaagc gggttgttgt agaaggggat cccttccgcg tggacggaga acccgccggt
    12601 ggagatcccg acaagagcga tattcaccgc gtcccagagg ggcaccccgg atacgaggac
    12661 gaggccgatc gaggcggcgg tcagcaccag gtagatcttc cacatctcaa agcccgttgc
    12721 gacgacgctc ggcatcagcg cctccgagcg cccttccgac cggtagaggc ggaaccgggt
    12781 cagcccggtc cgcgaggcca tcgcgacggt gaaggcgacg atcccgatac cgccgagcca
    12841 ctgcatcagc gaccgccaga agaggatcgt ccggggcagg tcgtcgaccg accgcatcat
    12901 ggtcagaccc gtatcggtcc accccgacat cccctcaaag acagcgtcga ggtacgggac
    12961 gccgagcccg aggcagaacg ggagggcgct caccagcgcg atgatcagcc agatcagggc
    13021 gacggccata agggcggcgg ataggggggc ctcacgctct cgccggggta tctgggtaag
    13081 aaacgtcccg aggagaaaca gggtgacggg gacggagacc atgggcagga tcatatccca
    13141 ctcccgatag atcaccgcca cgataagcgg caccgccgtg gcgatgctca tgaacctgaa
    13201 gatattgccg atgtcgggtg cgatgatcga gaactgctcg atcctatcca tcgatacaca
    13261 ttctccgggt gctcttatct actttctcct gcacgttcgt tttgctcccg gtgacctgag
    13321 atctcccaaa atcaccgacg gccgcattcg cagccaccgt tcccggtcga cgccgcctgc
    13381 tccccgccgg ccgtcaccgg cgtgctctcg atgaagatgc gccggttgac gattcgccag
    13441 gcgaggatga gctgggccat atccccggcc gtcaggtagg cgccgctctc gacgaggtcg
    13501 ataggtcgca cctgcgtctg aatctcgata ttcttctcca gtttcctcac cacggcgtcg
    13561 tttaactccc cgtcgaacgt gagtgccccc atcgccctcc ccccgatctc ttcgacggtg
    13621 aactcgacca tgaagtcccg ctgcgagagg gagaggagag gaaagaggtc gatggagagg
    13681 tcaacgtccc ggatgctttt ctcgagtttg ctctggcaga gggtgacgtg gtagacgttg
    13741 cgcttctcgc ttgcctcccg gccgtacttc gagaaggcga tccggatgac cgcgtctgca
    13801 aaccggcact ggggtgcgac gtactgctcg aagtcgggtt tgcgctccgc catctcccgg
    13861 aggacggcct caggggagta tccccgccgc tccacgtcgc gcttgatctt ccaggcacgt
    13921 ttgacgtcgg ggtccgggtc cacatagagt ttgaagtcga tcaggctccg catctttggg
    13981 gttataaacg ggtgcagccc ctcgaggatc aggatcttcg tgggtttaaa gggcaccggg
    14041 ggctcgaact tcccgttgtc atggttgtag accggttttt gaatcgtccg ccccgccttc
    14101 aggtcgtcga ggtgctcctc taacaggtcg aaccggttcg cctcaggaac aagcggcgtg
    14161 atcccgagtt ccttgcgttc ccggcggtcg taccggtggt agtcgtcgat ggtgatggtg
    14221 gagacgagat cctcgccgaa gatctcccgg atagcccggg tgaacgtggt ctttccggtc
    14281 ccgctgtctc ccgctacgcc gatgatgaag acataggacg actctgcaat gactctcttg
    14341 aagtcggatc ggggcataat cagatcaagg agtggtcggt atccttagac tgttaagatt
    14401 tcggtgccgg gccgagacca tacccgggaa aaagagatgg gcgtagagcc taggacggga
    14461 tgatccccgc ttcctcgtgt ctcttcttga tctcgtcggc gagccggtcc agcgccgcga
    14521 agtcctcctc cttcggggag ccctggacgt agaccggttc aatgaactcg actttgagcc
    14581 ggccgagcat atccgtcaag gtatcgaccg tcttcccgcc ccagccgtag gacccgatca
    14641 ccgtcgcgta ccgggtcttc gggcggagga ggtttgcgag gtatgccgcg tagaccacct
    14701 gcgggtgggg gccgaagatg accgtcggcg acccgatcac gacggtcgcg gcatctacca
    14761 gggcctttgc aaggtcgccg gtatcggtct tcgtcaggtt gaacggctgg acctggatac
    14821 cgcgctgcat cagggcgttg acgaagtggt cgaccatcgc ctgcgtgctc ccgtgcatgg
    14881 agacgaaagg aagcaccacc atgtttttga cggcgtcgcc cgtccagtcc tggtaggcat
    14941 cgatgatcaa cgccggcctg tcgtagatcg ggccgtggct cggggcgatc acgtcgatct
    15001 gccgcgtcgc gagctttgcg aggtgcgcct tgatgctcga gcgaaacggc atcatgatct
    15061 cggcgtagta gcgcttcgcg gcctcgtaga ccacgccctc gtcggggacg tagagggaac
    15121 tcgttgcgta gtgcgagccg aagagatcgc aggggaagag gacccggtct tgctggaggt
    15181 aggtcagcat cgtctccggc cagtggaccc agggggcgat gatgaactcg agcgtcttat
    15241 cccccagatc gagcgtatcg ccgtcgccga tgacggtgac ccggtctttt gaaatatgga
    15301 ggaggtcgac caggaagtcg cggcacttct ggttcgtcac cagttttgct ccggggaacc
    15361 ggtcgagcat tgccggtatc gagcccgagt ggtcctgctc ggcatggctc gagacgatgt
    15421 agtcgatggc gttgaccccg agctggtcga ggttccccat gagttctgca gtcttcgtcg
    15481 ggtcgacggt gtcgatcagt gccgtcttct cgctgccccg caccaggtaa ctgttgtagg
    15541 tggtgccgtg ggggagcgct atcagttcgt cgaagagccg gcggtcccaa tcgatggccc
    15601 cgacggcaaa gacgcccggc acaatctcac gtgctgccat gtcctgccgc ctccatcggt
    15661 tgcgctccgg ctgttatacg gtgtatccag gtgtccgaca tgcttcatgc tccgtagact
    15721 ctgaaccgcc ccggcgcgca gtgctgcgcc ggtcggtgag cgtctatccg gtccgaacgc
    15781 tgataacggt ttcgctctat cctcaggagg aggggagagt ggtctgtctc ccctccctcc
    15841 ggacgacgtt cgcatagagg tgggcctgcc gggtcggttc cgggagccgg tatccccggg
    15901 cggtcgcgag gacgaggtcg accgcgggca ggagagcaat ccggtggccc accgagacgt
    15961 agaccggttt cgtcttctct tttgtccgca ccgccatccc gaccgtcttg ccgttatgga
    16021 aaaccgggct caccgacccc ctgccgggcc cgggctccgt cgccgttcca aaaagaaggc
    16081 tctttgccac cccgacggta ggccggtcga gcaggaggcc tatgtggctc gcgataccga
    16141 gcccccgggg gtgggcgatc ccctggccgt cgaagaagat gacctgcggc tcggaccgca
    16201 gcctctggaa cgcctcgagg agggcaggcc cctcccggaa ggtcaggagg cccggtatgt
    16261 aggggaaggt cgtctcagcc aaggcggaga cccgctcgac gacggtgagg tcggggtaac
    16321 tgagggcgac gacgaccgca tagatctcgt ctgagccctt catgtaggag gcgtcggcgc
    16381 cggccacaag ggagacctcc ccgatatcgc cggagaggct gatccgggcc cggagcgcct
    16441 tctgaagaag gatcgcttcg gccggcgtca ggttgaaggg gtgggtctct ctgatctcca
    16501 tggccggctc ctgtcgcatg gcgacatgca caggttcgga gggtagtata taattatcat
    16561 cagctgcctt cacaacaccc ggcctcttcc ctgtcatccg ggttgccgct gccatcgtcc
    16621 tcgtgggggt gctcgccgcc ggcaagaaac acgatggagg gcaacgggga gggccgggga
    16681 gctgcagccg gtgacgagaa gccccgtccg ccggtggtgg gaggacccgg ggctccttaa
    16741 ccccgctgcc gtcccggatc agcacaatcg gggcctgcat gcccgcaacc ccggaccccg
    16801 gcacccgggg gcgaggcatt tttatacgtg gagatggggt acggcccgga gagtcatctt
    16861 cggagtgtgg acggtatggc gcggtgggtg tgcagcgtct gtgattacga gtacaatgag
    16921 gaagcggggg acccggcaac cggcataccg ccgggtacgc cgtttgagga tctccccgac
    16981 gactggcggt gcccgcgctg caccgtgggg aagggagcgt tcgtgcgggt caacggagag
    17041 gaggaagagg tgaggacgaa tgaagaagac tacctctgaa ggagccgcca tgaccgtggc
    17101 cgacgttctg gtctcggaac tcgcggcatg ggggatcacc ctgtacttcg gtatccccgg
    17161 atcgtcgtcg ctcccgctcg tggacgcggt caggaggaac cccgatgccc ggtacatcgt
    17221 cgtccggcac gagcagaccg cagcgatggc ggcgtccgcc tacaacaagt tcacggggaa
    17281 gaccgggcga gcgtgctcgc actctccggg caggtgaagg cgcagtacgc cggtcccggc
    17341 ggcatccagg agatcgacca gaacgcgttc ttccgcccca tcacggtctt cagcaacacc
    17401 gtcaccgacc cggcgacagc ggtcaaactc ctcacccggg cgctccggta cgccatcatc
    17461 ggccgcggcg tcgcccagct ctccatcccg aacgatatcc agagggagcc gctcgagccg
    17521 gcctactgcg aacgcgagac ctgtctcccc acggtggcgg tagcccccac cgacgaggct
    17581 gtcagggtgg cggccggtca tcctcgcggg cttcggggcc atgggtgcgg ccggagcggt
    17641 ccttgacctg gcgaagaaga tccgggcgcc ggtcgtcacc accttccggg cgaagggtat
    17701 cctccccgac gagaacgagt gggttgccgg atacacgggc cgctcgggac accgcatgcc
    17761 cgcacgctcg tcggcgagtc ggacctcgtg atcgcctgcg gcgcgagttt ctccgacttc
    17821 accgggattc ccggggataa gccggtcgtt catatcgata tcgatccgat ccagctcggc
    17881 aaacacccgt tcgtcgcggc ggtctggggc gactgcgcca tcgccctgcc ccggatcctt
    17941 gaactggtcc ggccgcggga ggacccggcg gtcgggcagt ggctcatgga gcggaggcgg
    18001 gagtggtcac tccagctcga ccggg
//
LOCUS       DAJB01000182           18720 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_43011, whole genome shotgun sequence.
ACCESSION   DAJB01000182 DAJB01000000
VERSION     DAJB01000182.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18720)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_43011"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3717 a         5848 c         5746 g         3405 t
ORIGIN      
        1 ggttttaacc gggggtagtt gacggacaat gtatcactct tgatcctctg tcttctgcga
       61 accaatcccg gatcatgtca cgtaccgggc ggaccaaccc ggaacgagcc ccatagtggt
      121 tatatccggc aacgatcttc ttgaacgctt cgatccggtc ggaggaccac ccggtggcgt
      181 tccaccatgg agagcagttc ggattgaacc ggggcgatcc ccgagttccc gcgggcgccg
      241 gtgatcttga tcccgtcccc gggcccgggg atcgcgcccg cggcgttcat cgccgcgccg
      301 accggatcgc cgttcaggat cgcctgcacc gcatcgcgga tgtcggcgat gccgccgacg
      361 accggcaggc acaaccctca ttatcaccgg cgccaatcac tctacaatga agccccggga
      421 cctctctatc gaggagaagc agcaactctc cgaccttctc gtcgggctgc tctcaaatcg
      481 agaggagatt ctctttgcct acgtctacgg gtcatttctc cacggtgcct tccgcgacat
      541 cgacatcggc gtcttcctgc aggacggcag gggcggggcg gccgagccgc ttcggtacga
      601 gatggcgctt gagcaggatc tggaagatac cacgggagtg cccatcgacg tcagggtcct
      661 gaacgctgcg cccctgccgt tcgcctactc ggtcctgcag accggggagg tgctcgtctc
      721 ccgcgacgag aaggctcgct gcgacttcgt atgcaggatc tatacccact accacgattt
      781 cgcctactac cgaaagcgtt accggaggga agcacttggt ctcctacgat gatgaaaaag
      841 tcaccgcact ctcgtcggaa ctgacgcagg cgtgcgggcg cttaaaagac ctcggccgga
      901 tgcaaaaaga gattgttatc aaagaccctc acctggtcgc cagcgcaaaa taccacctca
      961 tcgtcggcat cgaagccgcc atcgatctcg ccaaccacct gatcgcgaag aaccggtgga
     1021 gagcgccgga agactacgca gatacatttc gcatcatgga ggagcagggt ctcgttgacg
     1081 cggagttttc agttcgtctt caaaggatgg cccggttcag gaaccgtttg attcatatct
     1141 actgggatat caacgacgaa accatctaca gcctccttca agaagatgtt tgcgacattg
     1201 aggagtttct ttccacatac ctccgttttc tcaggacgga atgaggggcg gcattcatat
     1261 attccccccg ggtgccagcg agaagcgtcg atgaactgcg gattcccggc attcaggcgc
     1321 cccggacagc gacactcgct catccccgcc gcccccgcga ctcccccacc aggtaccccg
     1381 catacgggac ccccgacaat ccccgcaccg ccccgtagct taccacggcc gccgccgcga
     1441 agatcaccgg gtaggtcagc cagtcggccc acgagagccc ggtgagggag aaccaggcgg
     1501 ccgcagcggc ggcgatgacc agggggtgga tgaggtagat cccgtaggag tgctccccaa
     1561 atgaccgggc ggcacccgcc cgaaggcccc cctctccctc cagccgccag gccgcgagga
     1621 cgagcacggc gatgaccggg acgtagtaga aaggttcgag gatccggtag acgcagaaga
     1681 ccgcgagcga ggtgccggcg aggctcccgg agatgaggac gctcgccatc cacatgccgc
     1741 cgaggagaag ggcgcccccg ccggccgcgg cgagaatccc cgcaggggat aaagaccgga
     1801 ccacggaacg gaaccggtcc gtatgccggg cgacgtggat cccgaggatg aagtagaaga
     1861 ggtgcgaggg aaagaggcgg atcagcaccg tataccactc cgtgcctgca aacgcgccga
     1921 ggagatgtgc gccgacgttc cagaggacct ggaagaagag gagcgaaagg aggaggtaga
     1981 gcgccgcccc gctccggttg aaccagtcgt agccccggac gatcagcggg tagaggaggt
     2041 agagctggat gatcagcacg aagaaccaga ggttgtaggc ggcggtcccg aggaggaggg
     2101 cttttgccac agcatcaggc gtcggcaccc cggcaaactc aaatgtcccc tccaccgcca
     2161 ccaggaggta gagagcagtg aagaagaggt agggaagaag gatcgtcctc gcccggcggc
     2221 gatataagtt cgcaatcggg taatcgtcca cataccgcgc cgcgagcacc cacccggaga
     2281 tgaagatgaa gaccggcacc gcgaagtgag cggcgatgta gacgaagacg tccaggagtg
     2341 cgagcaggtt cacgtcgggg atccgggtgt agttcatcga gacgtggacg gcgatcacgg
     2401 cgagggcggc aaacccccgc aggtactcaa cctcccgaaa ccagaccact gcagccacac
     2461 ctcgccatcg ctcacccggg ccggcaagca ctacaggatt gccggccggg atatatgaac
     2521 ccctgcccgc cggccttgcg tagccggagg gctgcgggtc aagcactata ataccagaag
     2581 acaccaccta tctggagagg gagtccatgc agtactcgga agggcaggtc ggcagggtct
     2641 ttacggtcag gatcgacgac ggggaggatt tcatccgcga gatccagcgt tttgtaacgg
     2701 caatgaacgt ccagagcggc atgatccatt tcctcggcgc cgtacgatct gcaaagatcg
     2761 tgaccgggcc aaaggagccg gtcatgccgc cggttccccg gcgcgaggag atcttcgggg
     2821 gctgggaact cctcgggttt gccaccatct acccgggaga ggaggagccg tccatccata
     2881 tccatacggt ggcggggaaa ggaatacggt cgcttgccgg gtgcctccgg gagaaggcgg
     2941 aggtctacct ggtcatcgag gcgatcgtca cggagttcgt cggcatctcc gcaaaacgga
     3001 tcctggatcc gaaaacgggt gtcaacctgc cggtctttga ccggatactc ccatgaccgt
     3061 ccagccgaag ttcctcccca tgacgatgga ggaggcagga aggctcggca tcgagcggtt
     3121 cgacgtcatc ctgatcaccg gcgacgccta cgtcgaccac ccctccttcg ggaccgcgct
     3181 gatcggccgg gtcctctggg acgccggcta ctcggtcggc gtcatcgctc agccggactg
     3241 gcggaatgac gccgatttta agcgcctcgg cgagccccgg ctcttcttct cggtctcggc
     3301 cgggaacgtc gactcactgg tgaacgcctt tacgccgaac ctgaagcgcc ggagttcgga
     3361 tgtctactcg cccgggggga ggctgctgcg gcccgaccgg gcgaccctgg tctacaccga
     3421 ccgggtccac gccctcttcc ccaacacacc ggtcgtcatc ggcgggatcg aggcgagcct
     3481 ccggnnggcc gacctcctcg tcttcggcat gggcgagcgg caggtggtcg ggatcgcgga
     3541 ccggctcgcc gccggcgagg cagcaggcgc cctcacggat atccccggca ccgcctaccg
     3601 ggtcgatctc aagacctggc ggagcatgga ccaggccggg tacgtcgtcc tccccggtta
     3661 cgcagaggtc aaagaggacc gttacgccta cgcccgtgcg tttgcgctgc actacaacga
     3721 acaggacccg ctaaggggcc ggagagtggc acagccgcac ccaaagaccg tcgtcatcca
     3781 gaacccgccg gcgatgccgc tctcaggcgc tgaactcgac cgggtctacg aactccccta
     3841 cacccgaagg gcccacccct cctacaccga gcctgttccc gccctcgaac cggtcaggtt
     3901 ctcggtcgtc agccaccggg ggtgctttgg nntcgatcgt ccgggaggtg gagcggatgg
     3961 cagcgatgcc ggactttgcg ggcgtcgtcc aggacgtcgg cgggccgacg gcgaatatgt
     4021 acgccatcca ctgcggccgg tgggagaccg ccggcacctg tcccgaccgc cgctgcatcg
     4081 actgccccac gctcgaccgc agccacgagg agcagctccg cctgctcagg cgtctttcgg
     4141 agatcccggg ggtgaaacgg gtcttcatcg cctcgggcat ccgctatgac ctgatccctc
     4201 cagaggaccg ggagtacctc gcccgcatct gcgcccacca cgtctccggg cacctcaagg
     4261 tcgcccccga gcacatctcc ccgcgggtct ctgcctgcat gggaaaaccg ccccgcgagg
     4321 tcttcgacgc cttcagggaa cggttcgagg ccctccaggc agggaagcgg aagcggcagt
     4381 acctcgtgcc gtacttcata tccggccacc cgggctgcac gataggagat atggtcgaac
     4441 tcgccgagta cgtccgggat accggcctct acaccgaaca ggtccaggac ttcacgccga
     4501 cgccgatgag catctcgacg accatctacc acaccggcct cgaccccttc accctcgagg
     4561 aggtctacgt cccgaaaggg cgggaaaaac gggtgcagcg ggccctcatg cactaccggg
     4621 acccggagaa ctacgctctt gtctgcgagg ggctccgcgc ggccgggagg gaggacctca
     4681 tcgggaatgc gtggaactgc ctcgtccggg agaaacgagg cggtgcgccc ccggcgagga
     4741 gaaaagggca aaggtcgtaa ggctatcccc gcaggatcgc acccgccgca aacccggtga
     4801 ccaccgcgag ggcccggttc gggatccggg ggatgaggag tgtcgtcacc gcgaacgtgg
     4861 ccgcgatgtt cgccgtcgtc ccgggggtga caacggcatc gagcatccgc atcgtgccgg
     4921 aacgccatct cccgtgagag accggcgggg agagcgtgaa cttccctgcg aggtcgctcg
     4981 ccgtccgcat agccgtcgac cgccactcgt ctctcactgc tacactccga ctgcaggcca
     5041 tgatcgtagg gtgtggcccc gggtactact tattcctatg gcaggccata ccggcgacgt
     5101 gctcgcggat ccggagcatg cggttccgag gagagagggc ctcgagtgtg tggggaccgc
     5161 cagcgacccc ccaagggcga ttctcgaaga actgtccccg aacaggcaat ataccgtcgg
     5221 ggcccggaga attttcccgt gaaatgacgg aaaagcctat tgcttaccga caaaaaatgc
     5281 atcagcatgc attacgaaat caccggagac aacctgcaga tggtgaccct ccgtctcacc
     5341 cagggcgaga gcatctgcgc cgaggccggc gccatggtca acatgagcgg gaacatgcag
     5401 atgaccacca acatgaaggg cgggctcttc aagggattga aacggatggt gaccggcgaa
     5461 ggcctcttca tgaccgagtt caccccgacc ggcggggagg ggttcgtctc cttcgccggg
     5521 aacgtcccgg gcaagatctt cacgctcgac cttgccggga atgagttcat cgcccagaag
     5581 gacgcgttcc tctgctccga gcagggtatc gacctcgata tcgcgttcac gaagaagctg
     5641 cggtcgggcg ccttcggcgg cgaggggttc attctccagc ggctcaccgg cagggggacg
     5701 gcgttcctcc actgctgcgg cgacatcatg gagatgacgc ttgcgcccgg cgaggtcgtc
     5761 agggtcgaga cgggcctcgt cgtcgggttc gagagcaccg tcgactacag cattgctctc
     5821 gccggcggcg tgaagaccgt cttcttcggc ggtgaggggc tcttcctgac cacgctgacc
     5881 ggtccgggcc gggtcgtcct gcagtcgatg gacgtcgcga aactcgccgc cgccctgatc
     5941 ccctacctcc ccgtccagca caactcatcg ggaaggtaac ctgatagtcc acggcgtcgc
     6001 aggagtacct gatgaacctc gtcatggtgg tgcacggccc cgaggccttc gatgccggcg
     6061 acgcagcgcg actgatcggc ctcctctccc cccggcgggt gctggtcgcc ggggtgatgg
     6121 cccggaccgc cgccgaggag tccgggctcc cggtcacctg cacagacgag cggccgagcg
     6181 tggtcctcgc cgcgcttgac gggtccgcat gcctgatcaa ccggggaaag accccggagt
     6241 cggggcggat attcggcgag atcgtcgctg gcaggctcga agggctcgtc cacgtggaga
     6301 cgtcgagcgg aatcgtctac tgttggaacc atgccgatga gagcctcgca cgcgagatcg
     6361 cacagctgac cgggtacgct ctcgcgcccg gaacgagcac cgccgcccgg ggggaaggcg
     6421 tccgggaggt ccgtggatgc ctcccgggag aggcggtctt cgtgaacggg atcgtgatcg
     6481 ggaccgcgac ggcggagacg gtcgtcctct ccggggacgg caccttcagg gccgtctccg
     6541 gcctcgaccc aaagcctcac gggtttgaga aactcctccg ggccggcctc ccggacctcg
     6601 ccaccgcctg gtgcaagagc ggaccggtgc ggacgatccc tcccctgcag ggagagcgca
     6661 cctcccgcgg gggcagggtc gcggtcatcg accactgcgg ccacaccctc taccacgaga
     6721 tcggggaggg agtctgcggc gtcctcgccg tcggcgacga caccaccgcg gtctgcggcc
     6781 acatctgctc ccatgccggc atccccgtct tcggggtggt cgacggggac gccgacgcca
     6841 tcgtggagcc cgggtatgcc ccggggtcgg tggtggtcga ggtcgtcgac gggcgggacg
     6901 atgacctcgg gcgggaactt gcggcgacga gggacctcac ggcagccagc tgggaggagt
     6961 gggtggagga gacgctgtgc accctcgtag gaagggtgcg gatcgtcgtc gaccgccgcg
     7021 gaggataagc aatgcgatgc gccgagtgta aggggaaagg gctctgcggc ctcgaacgct
     7081 gcccgatcat gagcaggttc tacgcccagc tcccggtccg cccttccgac cactacgagg
     7141 gatccgctcc gtcggtcttt gtggggagtt acggctaccc gaaggtggcg gggggcccgc
     7201 tgatgatcaa cgacgccgac cacccgccgg actggatcgc ccgggggctt gggatcgacg
     7261 atatcgtggg gctccgggcc cggacgatac gcggcgccgg cgaggcgaag gggctcgccg
     7321 ggaacctcca ggagatcgcg ctctcgagca gcccgctcga tgtggaggtg cggtttgtaa
     7381 aaccggtcgc cttcgacctc cggttcgacg ggacgatcgc ccccgtcggg ctcaccggcg
     7441 ccatccggaa gatggacgtc ctcgggaacg cccgggtgga ccgggcggtc gaccgggcga
     7501 cgtccgatac cgacctctcg gcgaccgatg cctgcgagat cctcaacgcc tcggggaccg
     7561 acgtctaccg gatcacccag ctcctcaccg ccggcctcct tggcaagaaa cggcgcgtcg
     7621 tccccacccg gtgggcgatc acggcggtcg acgacacggt ctcgacccgg ctcaagaaga
     7681 agatcgcccg gtatcccccg ctttccggga tagagatctt ctccgcctcg ctctacggca
     7741 accagatcgt ctgcctcctc gtccccgggg actggaagta cgagatgatc gaggtctggg
     7801 ggaagcagtc gctctggggc gaggggggcg agacgattgc ccgggacggc gaaggcctca
     7861 ccaaatccgg ctactcaccg attgcaggcg cctactactc ggcacgtctc gcggtcacgg
     7921 agtacctgga gagcgtgcgc cgctcggcac gggtgctcgt gctcaggaac gtcacgagcg
     7981 attactgggc gccgctcggc acctgggtcg tccgggaggc ggcgcggaac gccatgaagg
     8041 gtccaaaaac cacgtgcgcc actctcaacg agggggtcga cctcgcctcc cgcctcatcg
     8101 gctttgccca ctggcgcccc cacagccggc tcgtccccga actcgtgaca cagaagacgc
     8161 tctttgattt ttgagtatca taaggtgcga gttgaggggc cttggggggc ctcactcgaa
     8221 gaccttcggt cttctcatgc tccggttcta gcgaaccatc gcaccctgcc catttttctg
     8281 gaggccgtag cgcccggcga gggtatcgag cgtctcgacc tcgtccacgc tcttgtcctc
     8341 gcgcacccgg acgaaccgcg ggaaccggag cgcgtagccg ctctcgtagt tcgggcttgt
     8401 ctggatctcg gagtagccca cctcgaagac aacttccggc tcaagcgtca cctcttttcc
     8461 ggaccgggca atcaccttct ccttgaagag ggcgtaaagg tcggccagca cctcgtcggt
     8521 gatcccggtc gccaccttcc ccacggggag gagccggccc tggtcctgga ccgcgagcag
     8581 gaaggagccg aacatcttcg cccgccgccc ttcgccccac tcggccccga cgaccgcgag
     8641 gtcgagggtc tcgaccccgg gtttcacctt cacccagagg cggccccgga cacccggggt
     8701 gtagggcgaa tcgaggacct tcaccatcac gccctcgtgc ccgagattca gggcctcggt
     8761 gtagatcgcc tccgccccgg ccgcgtcgcc gacccggaac tgtggggcga cgtgtgcgcc
     8821 gagcgcccct tcgagggctt tgcgccgctc cgcgaggggc cggtccatca gcgtctcgcc
     8881 gtccaggtag aggatgtcga agatcctcgg cacgagttcg atcttctcca tcatcgagtc
     8941 aatctcgtgc ttgcgccgga accgccggat cacgaactgg aacggcatgg gcctgccgtc
     9001 gcggacggcg accgcctccc cgtccaggat gacgtcgtgg tcggtcgcct ccagaagttg
     9061 ccgtgcgatg tcggggaggc tccccgtgac ctcttcgagt ttccgcgagt agatccggca
     9121 gacgtcgccc accttgtgga actggaacct gctcccgtcg tacttgtact cgaccgccac
     9181 ctcgccgtgg tcctcaagct gtgcggcgac ggtgcccgcc tgcgcaagca tcatcttcac
     9241 cggccggaaa ggctcgatcg tcacgcgggc gagttcgtcg gggttctgcc gcgcaaggag
     9301 cgccacctcc ccgaggtcgt tcatcgcctg gtgggcatgc tcgaccaggt ggacatcaag
     9361 ctcgaacgcc tttgcgacgg cgtcgcggac gttcccctcg cccatgccga tccggagttc
     9421 ctcgagcatc agccgtgcga ggtagcgccc ctcgagcggc cgggcgttcc caaagagccc
     9481 ctgtgccgtc cggagtttct cccgctgcga ccgctgccct tcggcggccg ccatccgctc
     9541 gaactcccgg tagacctcga caaggtccat ctcctggatg aagaacgacg tctgctcttt
     9601 cctggcaagg agttcctcga cggcgagacc cgcatcgccg gtcgtgttga tagcctcgcg
     9661 gaccgtctcc cgcttcgtcc cgacaacgta ggccacggcc tcgtagagga ggttcggacc
     9721 cacgccgagt tttttcgaac tccagtcggg aaagaccctg cccatgatga accgaacgaa
     9781 gacggggagt tcctcgtcgt cgagccgggg caggacggcg gcgacctgct cgatcatatc
     9841 gaggcgcccc ggtgtccctt ccagctgctc gcagacgcgg gcgaactcca gaaattgcat
     9901 acccatagtc tcctgaagca tttcccggcg gggggcatca accctcctct ttgacacgat
     9961 tgatgtggga gcactgccta cctctatcca tgcaccagga cgaagaaacg aaagagatgc
    10021 ttcgcgacct cctctggctc aatgccacga tagccaccga actgatccag atcaccgaga
    10081 acacctccca gattctccgg aaggcggctc cgccggagac ctgcatcgcc gaacatgcgg
    10141 ccctgcgggc gacggcgctc gagatcgccg atcgttacaa gcccggcacg atgctccggc
    10201 agcacgtcgc agagcaccag taaaaaaaga tgacgtggtc gccgtcaggc tccggccggc
    10261 accagtcccg catccctgac gacgaggtcc gtcgcctcga tcacctcttc tgtcaggttg
    10321 atgacggtgg tcgtccggtt gaccgggtcg acggtgctcg ccgtcgcgcc gggtgccccc
    10381 cggccgggga cggtgaaggc gtacccctcc ggcaggacga cttcgacggt gtagttcccc
    10441 gggaggacgc cgctgaagag gtagagccct tcacaggcgt gggagaacgt ctccgccgtc
    10501 gcgatcctct cccccgtggc gttctggagg tggacgacga taccgctcat gccccactca
    10561 ccggcgtcct gcacgccgtt cgtgtcggca tccagaaaga ccgtccctcc gatcgtgccg
    10621 atggtctgtt cttctcctgc acccggcccg gccgggactg cggcgcttgc gatcgaaccg
    10681 aagacgaacg ctgatacgat gattattcca agaatgctga tagatctcac tcggttcacc
    10741 tctttttccc ggacggccct cccgggcgct ccgggtatgt aacccccggc ggaaggctgc
    10801 aacgcctgcc ggaagatact gcaggcagag gacggaccgg ggtttttccg atccggtgcc
    10861 tgccgggggg ttcgacccag agcagctgtt ataatatata aaagttctca aatggctccg
    10921 gcagatagta actacaactg gccagaatga gaacccggtt ttcagccaac ctacctgaaa
    10981 atggacaata ccaggtttga ccacccggca gatggcagat ccccatccgg cgtcgcggca
    11041 ggggcatcgc gcgtattttc gccgtcaccg gcggttccgt tctccggacc cggcgtcacg
    11101 gtcggggtcg gttcctcggg gggtgtcacg gtcagggtcg gctcctcggg gggcgtcacg
    11161 gtcggggttg gctcctcggg gggtgtcaca gtcagggtcg gctcctcggg gggcgtcacg
    11221 gtcagggttg gttcctcggg gggtgtcacg gtcggggtcg gctcctcggg gggtgtcacg
    11281 gtcggggttg gggccaggga gaggatcaac cccgcatccc tgaccaccat atcacccccg
    11341 ccaaccgtaa acggggccgt cgtcccggtc gacggatcgg cgtggctgtc gccgccaatg
    11401 ctcgtgaaga tgtagtcgtc cggcggggtg aacgcgaggg agtactctcc gggcgggagg
    11461 gggccgaaga ggtacataga gaagtaactg tcatgatagc cggtgcgggc cgatgccgcc
    11521 acattcccgc ctgcatccag gagggtgacc tcgacgtccg tcaggccgtc ggtttcgtcc
    11581 cagataccgt cctggttacc gtcgctccag gtcgtcccga gcacccaccc gtaggccgcc
    11641 gccggggtca gggggaaggg actcccggtg aggccggcgt tcaggatccg ccgcccgtcc
    11701 accacttcgg catatgccac attcccgtcg agaggagcgg catggttgtc aagccccgag
    11761 atggtgcagg agaagccgtc cggcaggacg aactcggtgc cggcgtacct gccgggggcg
    11821 acgtcaccga accggtacga cccgtccggg ccggtgatgg tgtagtccat catggtccgc
    11881 tccccgatca gccgcacgct gatgccgggg atgcccggct cgccggggtc gagaatgccg
    11941 ttagcattca ggtcgtacca gaccatcccc aagagcgaat cgctgttgtc gtccccgtta
    12001 tcatcgtcgt cgtcatcatt atcctgcgtt ccggtcggtg acggcatggc cgtcgccgtc
    12061 accgtcggcg cgccggtggg taccgttgtc caggtgggcc cggacgtcgg gaaccccgta
    12121 ggcactgcgg tcggcgccgg cgatgcggtc gattccggtg gtgcacccgg caccggcggc
    12181 tcccgggaaa caatcggtgc tttcgagggt ttggcaggtg ccgccgtggg cgcgggcgtc
    12241 ggggttgcag tcggcgtggg cgtcggctcg gggcgaggtc ccagtctcac cgcccaggcg
    12301 tccgtattcg gccctcccct ctccgtggcg ttgaaggtcc ctgcaaagac gtgctctccc
    12361 ggtgcgatct ccacgatgga tgcggcctta tcgccgggat tttcaccccc gaaggtccgg
    12421 ctccaggcca ccgccccccc ggcgtcggtc gcaatcagcc aggcgtcggt gtcatcctca
    12481 ccggggtaac ccgtctcccc ggcaaagagg tagccgccgt ccgcggtctc gatgacggct
    12541 gcggccgact cgttcacgcc aaagtcgccg tagatccagt tccaggtgat attcccgtca
    12601 ggcgtgagtt tgatgaggag ggcatcggta tcgaccgtcg tgttatcggg ccgctccgtg
    12661 aacgtaccgg cgacaagcag gccgccgtcg gaggtgttga tcaccgcccg accggcatcg
    12721 tcgtcggggc tgccgaacgt cctgttccag atctccgtac ccgactcgtt cagcctgacc
    12781 acccagacgt cggccatgcc cgctcccgag gacccggtgc tgccggcaac ggcgaagtcc
    12841 ccgccgggaa gccgggcgac ggcatccgcc gtgtcgttgg ccccgccgaa cgttctgttc
    12901 cagacctcag cacccgactc gttcagcctg accgcccatg cggcggactc gtttcctccc
    12961 cggggggtga tggagccgac gaagaggtaa ccgccgtcat cggtcccggt caccgcgtgt
    13021 gccgaggcat tgacatccgg accgccgtag gtccggttcc agacctcgct gcctgagggg
    13081 tctatcttca cgatccaggc atcggtgtcc gcctgcgttt cgttcgtgac gagggtgagg
    13141 ttcccggcga agagaaggtt gccgtcatcg gtctggatga tcgagcgtgc ggtgtcggcc
    13201 tcccccccgc cataggtccg gttccactcc tcagtgccct ccgatgtcag cctgatcacc
    13261 caggcatccg tcttgccctc gccggacgat gccgtctccc cggcgaggaa gaatccggcc
    13321 cctccgggtt cgaaaacgat tgcgtatgcc gaatcattgg catacgtctc tccgtacgtc
    13381 cggttccaga gaaccgtagg gtctgctgcc tgtgcccccg cggccgcgat gagtgcgagt
    13441 gccgacagga ggatgatccc gaaatacgtg ccgtgttgca tggtttacct ctcctcgtct
    13501 ccatccccgg caaacgtgct gcactccata ccggagaccg cgggtatccc ccatctcccg
    13561 gacttatcgc gtaagacccg ggttttgttg tggaagaccg ggtcccctgc agagttctct
    13621 ggtcccgcag caccgcgggg tctgcacttc aatagcgcta ttattataat aattttattt
    13681 caaaacacgg gtgtggcagc agttcgagat caccgggcac cccggaaccg ggcaccggga
    13741 gttgctctcc tctccgggag gggccggcgc atatgccggt tcggttgccc tccggccttc
    13801 catcggggcg aggaggctcc cctgcgggtg tgacggcacc tggtgatccc gctataccgg
    13861 cgcatcccct ctaccccgca accatcaata cgcttatgaa caaaccttga taggagaccc
    13921 atatgaaact cttgcttgcg gttgcacctg aacggttcag ggatgaagaa ctcgagaccc
    13981 cgaagagggt ctttgaggag gcggggatcg atgtcgatat cgcctccacg gtcgccggca
    14041 cgtgcacggg gatgctcggg gctaccgcgg aagcggccat ggcgtttgac gacgtggacc
    14101 ccgacgacta tgtcgggatc gtggttgtgg gcggcgcagg ttcacaggag cacctctggg
    14161 gaagcgagat acttctgacc cttgttcagt cgttctttga gcagggcaag gttgtcgccg
    14221 ccatctgcct cgcgccggtc gtgctggcac gggcgggcat cctcgccggg cggcaggcga
    14281 cggtataccc gagcccggcg gcagtgccgg agatgaaaaa ggcgggagcg aaccttctgg
    14341 agatcccggt cgtcgccgac atgcagatcg tgaccgcgaa cggccccgga gccgccgcac
    14401 agttcgccga taccatcatc acgaaactcg aatgctagac gatacagaga gcggtgaggt
    14461 gaaccgggcg aactgcaccc tggtaatccc cgcatacaac gaagacggac ggatacggtc
    14521 gctcctcgag gatatctcgg gctttgccgg cgatctcgtc ttcgtttgcg acgggaccga
    14581 cgagaccgcc gccaccatcg gtgcgtttgc ggccgatcac ccgtcgctct ccatccggtg
    14641 cctgacgttc ccggcccgcc tcggcaaagg gggagccatc gtcgccggca tgaaggcggc
    14701 agccacgccc tatgtcggtt acatggatgc cgacggctcg acatccttat ccgagatgga
    14761 gcgcctcttc gaccgcctgg aaaccgcgga cggtgccatc ggatcgcgct ggctccccgg
    14821 ctcgatcctg acggtgaggc aggggttccg gcgccgggcc gagagccgtc tcttcaacct
    14881 gctggtccgg ctgctcttcg ggctcgatta ccacgatacc cagtgcggcg ccaaggcctt
    14941 ccggagggaa gcgctcgatg cggttctccc gtccatccgg tcgaccgggt tcgagttcga
    15001 cgtcgaactc ctctggcggc tcgcgcggag cggttgccgg gtcgaagagg ttgccatcac
    15061 ctgggagaac cgggacgagt cgaaggtcgc cacctcggac gcgagcgaga tgctcctcgg
    15121 gatgctccgc ctccggttcg ggtaattcat ccccgccgga acaaaccgcc gggttttaag
    15181 gttctccggg cgaatggatt ctgcaggagc agagatcacg gtgcttgaga aagcgatctt
    15241 cgttcaggga ctcagggagg gcatgcaggt cgatgcaccc tttgtggtgg atgctgcaga
    15301 cttaaaggag aaacggaacg gaggcaagta catccagctc accatctccg accacacggg
    15361 ccgggggtcc tgcaaggtct gggggacgcc cgaccacata gggcagatcg aggcgttcta
    15421 tcgcgcgatc gtggtcggcg aggtctaccg catccagggc tacgcgaagg tctacaacgg
    15481 cacctgcgag gtcaacgtca acgacgagat agccgatctc ctggccccgc tgccgcggga
    15541 cgggttcgat ccgtccctgt ttacctatgc acccgtcgat tgtgatgggg tcaagaggag
    15601 actcggcagt atggtagcaa agatcgaaga ccccgggata cagtccctcg ttctcgaagt
    15661 catcgacaac actccagggt tcctcgatgc gcccgcggcc aaacgccacc accacgccta
    15721 catcggcggc cttgcggagc acaccctcga tactgcggag attgccctct ccctcgcggg
    15781 caccgtcagc cgggcggaga tggataccga cgtgctgctt gccggagccc tcctccacga
    15841 catcgggaaa gcggcctgct tccgccgcca gggtttctcg ttcgtcgcgc tccccgagta
    15901 caccctcgtt gggcacacgg cccttggagc cgccgcgctt ctccggcact ccgccggcgt
    15961 cgatccctcg cgattcgcgc acatcctcca tatcctgatg tcgcaccacg gccctcacgg
    16021 cgaggtccag ccccggaccc ccgaggcctg ggcagtccat tttgccgaca acgcgggcgc
    16081 attcctccgg gggacgtgcg acgatgtcga gggtcttgcc ccgggagagg gaaggcaggg
    16141 agtacggacc gggaaggcag tctaccggtt ctgacagcac cccaacaggt gtcctctctt
    16201 ttagttgcca agcaccatta gatcctgcgc aggtaatccc ctccgtaacc agcactttgt
    16261 caatccctgc atgcttttgg aggtcgtccc aaaacgatct atgaaccttc agtgcaccct
    16321 gatcggaaca catacctccg aggtttttac catctgaacc agatcctggg caaggagggt
    16381 aaaacggctt tccattgagt atcgccacgc gggggagagt ccgggagggg gtgtccccct
    16441 cccaggaacg gtgttttggg acaacctctt gctcaagcca aacacttcag agtagagaat
    16501 acactgtgtg gagtgaccac gctagcggcc ataatgaaac ctaaactaaa aatgaatcct
    16561 gtaatcagcc gtaacaaatt attactctca cgataaccca tcgcttgcga caaaccgtct
    16621 agggccattg ggatcatgag tccgacagat aacagaaaat taaactggat attcagtaat
    16681 acagttacaa ttccaacagg tatacctgca acaattccaa cacatcttgc acaaagatac
    16741 ctatctaaac caaagaacgt gatacatctg tctttgcgtt tatgacaaaa acaaaatcct
    16801 attgttcgtt gagtggtgtg gaatctgacg accggaccga tcgcctcatc ccattcgtac
    16861 gagactctca caatactccc agatatcgtc taatggtatc aattcaactt ttagaaaaga
    16921 ttcaataatt ttccgaaatt gtttcgcgat gtgcggttga cttcaaccta taattcttgt
    16981 gttgttctct caaattaagt tgtaatgggg cacccaaaag attaaatatt atagatatct
    17041 aatagatatc taatgaaaaa agttcctttc aatatcgcaa cagagttgaa agtaaacaac
    17101 atctgtggat tctacataag agaagtcaaa cccttcggaa ccagcgcgaa agttgattgt
    17161 ccaaaagagc atttaggaaa aatcgtttat ttggtgatcc tgaataatga cgaataatga
    17221 caaaccagaa aataaggaga actatatcga tgagctagag cgaacaatcg atcagttgaa
    17281 gaaagaactt gaaatagaac gtaaccaaaa aaacaaactt aaaagggaaa atgacaacct
    17341 caaaaaacag cttgcacaat tacagggatc tgctccgttt cttgcagcat cttgtaaaac
    17401 tgctgaagcg gggggagttc ccagttcaaa aacattttac cgtaggaata ggcaaaatga
    17461 aaacaaaaaa gccaaaggtg gccaaccagg gcataaaggt catggtaggg aaaggccgac
    17521 ttcaaattcg ccgtttgtaa atatcacatt ggatacatgt cctgactgtg gtacacattt
    17581 gcacaaccct gtcaaaggtg cagaacaaaa acgcaccata acggacattc catttccaag
    17641 acacattgtt tacgaaatac gttacccaag atactggtgt tccaattgta agaagttggt
    17701 tcgcggagaa ttgcctttac cccccaacca acaattcggg cctgccgtat cttcgtggat
    17761 agcttatcaa aggatgttag gattgagtat agggaaaatc aaatccagtt tattcgagac
    17821 ctatgagatt cgtatgagtg aagctaccat tctgaaacta gagaagtggg tggcagatac
    17881 actcaaagaa gactacgaaa aactcaggga tgagatagta cgcggaaata acatcaatgc
    17941 tgatgaaacc ggattcagaa tcggtgggaa gaatggttgg ttatgggttt ttacttcaac
    18001 aatcggctct tactataagg ttgctccaac acgtgggcat tcagttccag aagagatttt
    18061 ggaagatttc aatggcgttt taggaagaga tgcatggaag ccatatgatg tcgttacgtg
    18121 ttcggggcat caacttgatc tccttcatgt gaataggtgg ttggagagag cagagattaa
    18181 acacaatata gaaccacgga gtctcttaac ctctcaacca gcaaaattct taaaaacaga
    18241 tagacctcct gaaaaggtta tcgagtttgt tgatgggatt cgttcgattc ttaaacgagc
    18301 gattgagtat acggaaaatg atccacctcc atctatgaat gagcggataa atgcatgcaa
    18361 agagttccag ggagagataa ctgcattcct tgatagagac tggtctgatg tagatgctat
    18421 ccgaataacc aaagaacttc gaaagcggca ggatatgctg tttacgttca tgcgatacga
    18481 ggatgttcca tggcacaata atgacgcaga acgggcaata cgtcagggtg tacttcaccg
    18541 taaaattagc gggggtcgaa gaacatggat cggagcagat atatttgggg tgcttttgag
    18601 tatttacgaa acctcaaaga aaaaaaagca gaaatttatg gaaatagtgg gagaaaaact
    18661 tggggtttca tcgagtgaga ggagtgcaaa caactcaact tcttaaaagt gagttgttac
//
LOCUS       DAJB01000183            6140 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_65973, whole genome shotgun sequence.
ACCESSION   DAJB01000183 DAJB01000000
VERSION     DAJB01000183.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_65973"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1295 a         2080 c         1794 g          971 t
ORIGIN      
        1 cgccaccgcg accggtattc catccccgtt ccccacgctg caccgcaccc ctacgaccct
       61 cgaaacatac gaccacatca ttatcgagtt ccacattaac cggaaagata ccgtaggagt
      121 cggctgggta gagggggacc cggaagccgg caaccgcctc ggcgtctacg gcctgcggga
      181 actccgggag gcactcgagt acgccgaagg tctcggagcc gaggccgtcg ccctggtcga
      241 aggtccccgg aagactcccg gcggataccc ggtactggtg atgctctgcg aggacccggc
      301 cggctctgtc agccgtcagt gcgttgtcgt cgcccctcgt gacgaggaag ataaggaggc
      361 ggatgcctga tgcgctgcaa acagtgcaac cacaaaatgc gggtcatcaa aggcgcctac
      421 gccgtctgcc tccgtggctg cggccgggtc gtctcgttcc gcaccggggc aatcatcccc
      481 ggcgacccca tgctcgcggg ggtgccggca tgatcccggc cgtccgcctc accccggaga
      541 ccctcgcgga cttcgagcgc cacctctcgg cccggggctg ctacaccccc gcgaccgtcc
      601 gcaacaaagt atccctggca cgagccctgc aatcctcgac cttcagcgcc accgggttcc
      661 cgctcgacga agacgccgcg atcgaccgga tccgggacac ccagagcccc ggccgccgcc
      721 ggcggaccct gaacacgacg gcacgagacc tgatcgcgtt cctccggacg agacacgcaa
      781 cagaggtggc ggcatgatcg aggtcatccg ctcctccacc gccctccccc ggacgcagac
      841 cgtgatccgg gccggggatg tcgtagtccc gtgggcgatg ccgaccgaac gccagcagcg
      901 ccgccggtgg cgcgagtact ccagaccccc ggcctgtgca gagggggatg cctgatgtct
      961 gcggacatta ctggtttcct ccacgagatc gcggcagcgg tcggagacgg ccccttcacc
     1021 gcgtcctcgc tggccatgcg gcggatcttc gtcccccggg gcaccaccct gcggcagctc
     1081 cacgccgccg gcctcctcgt gagcaccggc accgcctggc gtctcgcccc cggagtggct
     1141 acccgtacca acgatcaggt aaccgcgtag ggcgatccca atgtcatcgg acatcgcgga
     1201 gcgccctccg ctcatcctca ataccggccg gtggatctgc cggccgtgca tgcggcacaa
     1261 gacccacccc cgcttcatct acagcgacac cggcctcgaa atctgcccgg tctgtggcga
     1321 acgctcatgg gtgccggaat ggaacgccga agcgctagcg gaggctaacc ggcgacaggg
     1381 ggaagcatga gcgctcctgc aacctatctc tggtgcgggc ggtgcgggca ggctatcgag
     1441 cccgacgtcc gcgataacca gtggccgtac tgccgggcgt gccgggtgct ccccgttgag
     1501 gagcgaggct gcacgctcgc ggcggcgatg aagagcctcc gactggaaga gctccgaggg
     1561 gtgccggaat gaccctcgcc ctcaccatct acctcaccga cgagccgcta ggtgtcaccg
     1621 gatacggcac cgtcgcgatc ccatcggcgt acgtgtgtgt ggcggccgtg cttgcgttcc
     1681 tcatcggtat ctacctcatc acctgcctaa taccgacccc gaccgttcca ccgagggggc
     1741 ggcagccatg agcgaccggg agagcatctg cgaactcttc tgcggtgccg gcgggatggc
     1801 gttgggcttc gcccccttct tcgagatcgc gtatgccgtc gatatccggc ctgaagccgt
     1861 agcgacctac ggcgcgaacc accccgacac cgacacccgg aagcgcgacg tccggaacct
     1921 caccggctgc cggggcgact tcgccggcat caccgggatc atcggcggcc cgccctgtca
     1981 ggcgtggtcc tgccggaaca cccgccgggt cgaagacgac ccgcgggctc acctcatcgc
     2041 cgaatacctc cgactcgtcg aggagatccg tccaaacttc ttcgtcatcg agaacgtcac
     2101 gaccgtcccg gcgaaagaga agacggcagc catccgcaac agccgggcgc tcggatacga
     2161 catcaccgcg acctacctga acgccgccga ctacggagcc gcacagaccc ggcgccggtg
     2221 gatcctcata gggacccgga ccggaaccct cggcatcatc aaaccccgtc acccccgaac
     2281 cgtccgggac gccttcgcaa ccgtccggga aaactggggt ctcatgcagt ccaccccggc
     2341 aaccctcgaa cgcctggcac gagcccccac aaagcagtgg ggagcgatgg acggcgacgg
     2401 ataccggaac atgatccggc tccagtggca ccgtccggcc ccgaccgtct gcaacccccg
     2461 caaggtctac atggtccacc cggaggaggt ccgcaacatc tccctcgccg aggccgccgc
     2521 ccttcagggc ttccccggcg aatacctctg gaaaggatcg gacggggcga tcgcccagat
     2581 gatcgcaaac gccatgccgg ccgagatggc cgccgccatc gccggggcac tccccggcgc
     2641 aaccaccccg gaggccgtct aacccatgcc cgagcgccag cagaccttcc aggacctcgc
     2701 ggcgctcacc tacgtccagc gtctcaaccc actccagatc aggatcctca aactcctcgc
     2761 cctcggtgac tacacccagg ccgagatcgg gcggcgtctc aacgtcagcc ggcagtatgt
     2821 caaccagatc gtcagcaaat tccagcggta caacctcctc cagaagaacg gccgcgcctg
     2881 gaccgtcaag taccgccacg acgaccgccc ccaccgggaa gactacctca cccgggagaa
     2941 ggccgaagcc gccgccgagc aggcccggaa gaacggcatc tcccccacca tcgaaccctc
     3001 gcgaggcagc ccctacaacg ccacctactc agtaaacccg gccctcagaa aacagatcga
     3061 ccgggagacc gactacatcc agtacaccag ttgccgcgtc cacaacatcc gccggtccta
     3121 caccatcctc agacaatccg gccccgtcgc caccgacccc cgcaccggct acgaaaagag
     3181 ctggaacatg cgaggaggcg cccgccacaa attcttctac cccggcaaag gcaccgcccc
     3241 gaacatcacg atcgacatcc accccggcac cctcaccgcc tacccagacc gggggcagac
     3301 cgtcaccgcc gagagcatcc aggacgccgg cgaactcgta cgaaaagcca tcgaggacgc
     3361 gatcgccaac ttcatcgaag gacaacggcg gttcggcatc cagatcgaga tcatgcacgg
     3421 ccggcagacc acgccgacgc actacggctt caccttcagc accgacgccc cgcacgccgc
     3481 cgaacaaacc gccctcccgg gcttctggat cgaccacagc cccgaggcca acggcgaccc
     3541 caaccgcgcc gagatcgaga ccctcgaccc cacccgggca accggcctcg accgggccat
     3601 cctccagatg caccgcattg acagcaccat agcggacggc atcgccgccg cactcccgca
     3661 ggcaatggga ccgctcaccg cccaactctc cagcatcgaa gcggcggtct gcggcgggat
     3721 cacgatgcag cagaaggtgg acaacctcac catgatcctc gcggcgatgc tcaagaaaaa
     3781 tgagcagttg tgggacctcg tgctcaccaa cttaatcccc ggccagaccg ggcagaaaaa
     3841 ccacccccac caaagcccta caacgggcgg caacggaggc caccatgtca aagcataacc
     3901 ccaacacaaa aaccccgcca gaagcgcagg agcagggcac acagcacccg ttaacctgcc
     3961 actactgcgg gagagcggta cgtcgaaccg caaccgtaga ggcatacgat cgcacccggg
     4021 gccgctgcct gatcttctgc agcgagcggt gcaagcaggc tgcccgcaac cgcccggacc
     4081 actattaccg gttctcgcac gcccggatcc acacgattct ggcgcacgca gagggggcgt
     4141 tcgagtgatc aagacgttgc ggcactactc cgcggcgtgc cacgtctgcg gcggcaaagg
     4201 agtgcggcaa aacgagttct tcgagatctg cggcgggccg ttcgtctggc gctcccgacg
     4261 gtgccggatt tgtaagtatt acctattgtg caaccgggga gagtggcggg agtgctccgt
     4321 gtgccacggc aacggcaccg tccacctcta ctgcgtggaa gagccagcgc tggaagtgat
     4381 ggagctccag atccccctgg aggccgcatg acccctcacc gtcgctgccc ccatgaagtg
     4441 caggaggtcc gggagggcgt cttcgtcgcg gccgtcgatt atcacttcac ctgcgtctgc
     4501 cccgaccggg tgaactggaa ttgtgatcaa tacgtctgcc tctgcccgga acgccgggcg
     4561 gtcccggtgg actaacagat ttggtagaga agtgatatga ttacctctaa cacgccgatc
     4621 agggggcact acaagtacca tccccaaccc tcatctccta cactagttga ccaggcaatt
     4681 cgggccgccg gagtaagggg gctgcgagtc ctcgatccgt tctgtggaac aggaaccatt
     4741 gtgtgtattg cagccgccaa cggcgcaaaa atggcaatcg gttctgacat cgaggattac
     4801 accttctgct cccggagccc ggtctatatg gctgcaacct ctcaaggttt cggggatccg
     4861 gctatcgcgg tgcattatgg ggttgatgcg tttgaagcca tctcattgta tgactatgac
     4921 atcctgatca ctgacccccc caatcctcgt gcaatagtcg gcggcgcccc gatctcaatc
     4981 aaggagtata ccggaatgaa cggttgggag ttagccaaat tctggaaaaa ccgattctct
     5041 gaccgcaacc tgattaataa gaaggaggaa accgtatcga gagtacggga tcttttcgtg
     5101 caggccagcg ggcagggtaa gaggattatt gccaacctgt tcaactcaag agggtttgac
     5161 tattcaaagg cattgtgtga tgaatttacg ctccggccgg tggccgggac gtattatgag
     5221 atagacttgg tagaggagta gaagatggct gatggtatca cggtagacat ggggaagatc
     5281 caagcgtggc tcttctcccg tgctgagatg caccaggacc gcggcgagtg ggatgcgtcg
     5341 ttcgaggatt tgtacctcgc caccgcgatc ttccggggag acttcacccc ggatcccgag
     5401 gagtaaaagt atggtagagg agtgaaagac aatggaacat ctcagccttg gaaggccgat
     5461 tgaggcgagc ctcggccccc ggtatcaatg cccaatgtgc gggctgaaac tctggaccat
     5521 tcaggccccc tggactggct ggcaagaatg gtaccggact gaagacggcc gtcggcacta
     5581 caagcactca tgcaacatca tgcgggatgc tctcgtaatg ttccggtgga tgttcgggca
     5641 ggactggtag aggagtgaaa gcatggcaga atacaccata gaactgtaca aaatagacga
     5701 gcccgtgaaa ttaacgcggg tcgtctccgg tcccggtggc atcggtcttc atgagcgggt
     5761 cttcccctca gcattcgcgg cgtttacgtg gattatgggg gacgtatgcc ccccgggcga
     5821 tgatgtgatc acaattgagc acacagacgc cccggtcctg gtagaggagt gaaaggaatg
     5881 gaagccgaag tcacgcacat aacaaaggac atcaccggag aatggcgcga tatcggagca
     5941 cccatggatc agtggattca ggagtgccct tacaactgtg cttgcattaa gggtggcgag
     6001 tgtgagcatg agcgatatca gtatggctac tggccggtat gccgccatcc gcgatacctg
     6061 gagattttcg cgggcattcc aagcatgtgc tgtagcgagg atgagttaga ggacttggta
     6121 gaaaagaggt agttcaatgc
//
LOCUS       DAJB01000184           12608 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_28339, whole genome shotgun sequence.
ACCESSION   DAJB01000184 DAJB01000000
VERSION     DAJB01000184.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12608
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_28339"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    3533..3632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4200..4218
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2292 a         3928 c         3960 g         2307 t
ORIGIN      
        1 gggatggaga caggggaggg gggatgctcc cccccgtcag cccctccccc taatggcgat
       61 atcccctcga aatccgtgtt aaggaggcga gcgaggtctt cccttcaaac ttctcatttt
      121 ccccagttgc atctcacagc cttcgagtat tcaaactccc gccgagtaaa caggtattct
      181 ctggaccttc gccacctgat ctccggtgtc agaaaaactc cggatggata attatatatc
      241 cggccgcccg cacattccca ttaaccatgt acctcgtcat ccgctgcccg ggctgctgga
      301 ccttcaacta cgtggaccgc taccagcgtt ggaggctctg tcccatgtgc ggcgaggcga
      361 tcaacgtcga gcgtgcgccg gtctacctgg aagccgatga cttcctggat gcagaacgag
      421 tggtggcgca gctggagtcg tacctgcacc agaccgggaa aacagacctc acagagcagg
      481 acatccagca gttacgggcc cagtatgccg agtgggtgaa aaaccgggtc tgatcaccag
      541 agacgcccgc agcccggaca gagcatgctc actcgccttc cgcagtgcgg gcagaacaat
      601 cccgtacgtt cgttgctgga tagcggtctc ccgcatcgct tacagtgaat cggggcatcg
      661 aacccacact cgtgcctgac catcagtcca gatattcgta gtccttcaca aaaagatttc
      721 cttttccccg gttcgcgata tccccggtaa aacggataag cgtgcgaccc tcgtgctgaa
      781 ccgtcccgat ctttctgaag gtggggagca tcccctgcgc ggtcccgtag acgtagcagg
      841 agcccccttt catgttcgca cccgggcggc ggcagtcccc gtgaacggtg agcgtgccgc
      901 cgtgcatctc cgccccggcc atatccccgg catcgccgtg caccgtcacg gtgccgccgg
      961 cgatgctctc cgccaggaaa tcgccggcgc ggccgaagac ctccacggtg ccgccggtca
     1021 tgcccttcct gccgccgcgg tatcccgacg cacagtagtc ggcgacgtcg cccctgcaga
     1081 ggatggtccc cccggccatc tccctcccga gccagccgtc ggcgttgccg tgaacctcga
     1141 tggtcccgcc gctcatgaag ttgccgcagt gcatcccgat atccccgagg acggtgatct
     1201 ttccgccgtc catgtactcc ccgaccctct tcaaccggaa cgtatccccc gagaggacga
     1261 cctcaacatc ctccgtcctg ctggcactgc cctcgattga gaccgagaag acctcgtcga
     1321 gcctgagctc ccggttcccc cgccagacta aaagatcggc gccggcaagg aagttcttcg
     1381 ggacgatcga ctccgcctcg atgggaataa acgatttcgc ggcgggtttc atggcgagcg
     1441 tgaccttcat aactccgcct ccgtctcgat gcaccggttc cggttcaggt actcatcctg
     1501 caccgggtag ttcctcatcc ggaccgtgta gtagcggtcg aacttctcga tgaagtcagg
     1561 atctttcccc atatcataag cctccgagac cttcgggctg acccagaacg tggcgttgct
     1621 cccgtcgacc aggcactcgc ccctccgggc gacgacccgg ccccgcttga tcgtgtactc
     1681 ggtccggctg aacgcatcga ccaccttctg atagtcagtc gacgggtcga cctcgtcgat
     1741 ccggagcggg tagatggcga cgtccgcatc ggcgcccggc ccgagatggc ctttcccgaa
     1801 ctcctggatc ccgagcgcct tcgcctggcc ggcccgggtc atcaccgcga tctcgtacca
     1861 gtccagttcc cgctcgatcg ccgggagggg cacccgtgac gcggtatcga ggtggaccgt
     1921 ggcgaactcg gcgtcgcggt acttcttgct catcaaaagg ccgatgatct cggggtattt
     1981 gacgaacggc gcaccgttcg gattatccgt cgtgagcagg cactgccagg ggctcttcgt
     2041 gaggagcgcg agttctaacc caatcgccca catgatcgag ttgaccaggt tcttgcgccg
     2101 gtacatgacc ggaatgaccc ctgagccggt ctcgagctcc acgtcgtggt tgctccactt
     2161 ctcgtggtgg aggcggtaga ggttgaactc catcggcccg tcggcggtca tcgtcgtcgt
     2221 ccgaccaaac atcacctgtc ccatgtccat gacgatctgc gggcgcatgt tgacgaagtt
     2281 tgcaatcggc tcgctcttcg agcagatatc cctccaatcc gacccgccgt aggcgtggaa
     2341 ctggacgtgg gtggcataca gcgtctgccg cttcggattc aggtccggga tgaggtcgag
     2401 ggtttcgagc gtggtgctgt agttccccgg catcccgagg ttgttgcagt ggaggtgggc
     2461 cgagtgcggg aggtggagga tctcgttcgc ctcgacaacc gactggatga tctcggccgg
     2521 cgttacctcg aagtagggca ccgggtccct gatgccggag acgttcttgc cccactgcca
     2581 ggcctcggtg ccgccggggt tcgtgagttt gacgccaaac cccttcaccg cacggagcgt
     2641 ccaggcaatg atggccgcaa cgcgcttctt gtcgccgtcg cggatcgcct ccatgatccc
     2701 ccagttcccg tcgaagagga cgtttgccat cgtgtcctgg agcggggtat aggtgaactc
     2761 ctcgtgggtg tggcgggcct cgagcggcgc catcgcgccc tcgagcaggg tggtgtagcc
     2821 catcacgctg tagcggtagc tgttgccgta ggtggtcggg acgctgtagc ctgacgtggc
     2881 gtgcatcggc ccgcgccggg gcgtcctccc cgcccgcata tcctcggggc tcatgtaccg
     2941 cccgaagttc accttcgtcc cgcagatgtg ggtgtgcgaa tcgacccctc ctggcagggt
     3001 gagcatgccg tgggcgtcga tcacctccgc cgcatcgctc acctcctcga cgatcctgcc
     3061 gtcaaggatg gcgatatcca tcgtctcggc gttgatgccc cggagggggt cgatcacgca
     3121 ggcgttcttg attcggagtt cgttcatcgc tcttgcgcct ccttcatcag tgcgtacatc
     3181 cgtaagagga tctcccgatc ggacgggtag tcggtggaga ggaccttcct tgtccggata
     3241 ggcaccccgt ccatccggta ggccgtcccc tccgcatcga tcccggtcac cgccaccggg
     3301 atctggacgt tgcagaacgc ggtggtagcg ttcggatagg ggtcgagctg gaccaccggg
     3361 atatcggcga gatgattgag gcagcggcgc gggaagtggg cagccgggtc actcccgacg
     3421 atcagcgccg cgtcgcactc cttccgggcc aggatatcca ccgccgtcgt ctccccgggg
     3481 ttgtagaagg cgatcccccg ggagaagtcg atcccgaagg ggtagccggt cannnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccccg aacttcgccc ctttacaaag
     3661 gtttgccacc cggacgagct gctcccgggt gacgcccgcg accgtccgcg ggatcgtctc
     3721 ggtcttcccc tggacgatag cgcggagcgc ggagagaacc gcgtagtccc cgccgggttt
     3781 ganntctgga cgttgcagaa cgcggtggtg gcgttcgggt aaggatcgag ctggaccacc
     3841 gggatatcgg cgaggtgttc caggcaccgg cgcgggaagt gggcggccgg gtcgctcccg
     3901 acgatcagcg ccgcgtcgca ctccttccgg gccaggatat ccaccgccgt cgtctccccg
     3961 gggttgtaga aggcgatccc ccgggagaaa tcgatcccga aggggtagcc ggtcatccag
     4021 gtgttcactt cgttggaccc gtagacgttg tagtggcccc gcatcgccga gatggtgaac
     4081 ttcgtgtgcc ggctcagctc ggagacgagt tcgatggcgt ttcggatgtt cttgtacttc
     4141 ccccgggtca tcgtgagccc gagcccgaag tagaccgccc cgaacttcgc ccctttacan
     4201 nnnnnnnnnn nnnnnnnnac cgcgtagtcc ccgccgggtt tgatctgcac gaactcgtcg
     4261 gcgatgttcg cggtctcggt cttcctgacg tcgacgacga tcaccttccg gtcgcggaag
     4321 gcgttctcga ggaagaaccc ttccgcatag cgcgtgtagc gcgagaggtg acgggggtgg
     4381 gcgtcgatcg ggttcgaccc ccagtagatc acgaggtccg cccggttctt caccacgcca
     4441 agcgtgcacc ccgggtgccc gacctcctgg atggcgagga tcgaggggcc gtggcagacc
     4501 gaggaggtgt tgtcgatgac gccccgcacg agctcggcca tatgcacccc tacgcactgg
     4561 gcctccccgt gagtgccgct ccacccgtag agaagcgggc gctcggcgga gaggagcata
     4621 tcggcggcat accggatcgc cgtatcgtag gtgatatcct tccactcatt cccatcgcgc
     4681 aggatcgggt tcttcagcct tttttcgccc gtgaacttct tcgtcccgag ttcgcaggcg
     4741 ttcgtcacgg cgaccaccct gccctcctcg acttccacct cgatgtcgtc gcagaggcat
     4801 ccgcagaagg ggcagaccgc atccttaacg atcataagca agacctccca ggtcttccat
     4861 caactcgtga accctctttg gttcttcgtc cgtcggttcg atctccaccc tgtgcgactt
     4921 gaagtccggc atcccggtgg agtgggtatc ggcagcgacg atgtggtttg catacggccc
     4981 gtaggggaca aaaacggtgc ccatctccac ctcaaccgat acggacgccc gcatgacgac
     5041 ctccccgcag ggccccgtca ccctgacggt atcgcggtcg gcaagcccga gctccatcat
     5101 gtcgaactcg tgcatgaagc aggtcgaggt ctcttccgca taggcggcag aggtcttgtt
     5161 ctcgacggtc acaccctgat tcaccgtcct cccggtattc atgagaaacg tcatgcctcc
     5221 gcctccccgc cctggacccc ttccaccatg cgggcgaccc tgatggcgcg gttcgggcag
     5281 ttggtctcgc aggtcttgca ccccgtgcac ttctcctcgt ggagtatctt cacccggccg
     5341 ccctcgatcc gcatgatcac ctcgtcgctt gcggacgtcc cggtccctgc aagctgaggg
     5401 tccacctgcc ggttgaccgg gcacgagacg cagcagttcc cgcaccgcat gcagaggttg
     5461 tcgtcgacct ctaagcggta ccacgaggag aacgccgtcc cgtcacgccg ggtgacttcc
     5521 aggatccgcc cgctcgtaag aacgccctcg gggcatgcct cgacgcagag gccgcaccgg
     5581 atgcagtggc ccggatagac ctccggtgcc caccggtcct tatgccggag gaccgcgatg
     5641 gcgccgggcg ccggacagat catcatgcag gtgaggcatt gcgtgcactc cttatcggtc
     5701 agggtcgggt agtccttgaa gtatgagggg gttgtgagcg gcgcggtctt taccgagaag
     5761 aacttcttca accattccgg cctgaggaac tctcgcacgt agtagacgat cgatcgcata
     5821 tcctcacctc tcgttgcacg cgatgcaggg gtcgctgctg atgaacgtcg acgtcacgtc
     5881 ggcaatcgat gcggcatcga cgatcatcga gtgggcgcag gcctcgatgt tcatgatcga
     5941 gggcgtccgt atcgcgatat cgagcaccct cccaaagtcg tcggtcttga tggtgtaggt
     6001 cagctcgccc cggggggctt cgccggcata cgaggtctcg cccgccttgc agaggccgcc
     6061 gccccggatg gggccgtccg ggagggtttc gacgcacttg cggatcaggt cgatgctctg
     6121 gaagacctcc tggaaccgga ccatgatccg ggcaaagttg tcgccctcgg tccgggttat
     6181 ctgcgtaaag ccgagttccc ggtaggtcgg gtggcggagg cggagatcgg actcctcgac
     6241 accgctcgcc cgggccgtgg ggccgacggt gtgagaccgg atcgcttctt cccggctcat
     6301 gaccccgatc cccttgctcc ggagagcgat cagggggccg gtctcgaaga tccggacata
     6361 ccgcttcatc tcgtcctcga ccttcacgag atccgcaagc atcgccgcgg cgtcctccgg
     6421 caggaggtcg aaccggaccc cgcccgggat catataggcg gtgttgatcc gggagcccgt
     6481 gatccgctcg aggttgtcga gcacggtctc ccgggtgttt aagaggtaca tcgcgagcgt
     6541 ctcgtgctcg atcgtgtagc agtaggagaa gttcgcgagg atatggcttg ccatccggtc
     6601 gagttcgttc acgatgaccc ggaggtaggc cgcccgcggc gggactgcga tactgctgat
     6661 ctcctccatc gcctcgatga agaccatgtt gtggatgacc gagcagatgc cgcagacgcg
     6721 ttccgcaagg aacatcacct cctgccaggg ccggccccgc atgatccgct cgatcccttt
     6781 cttcatgtac ccgagctcga cctcggcccg gatcacccgc tcgcccgccg tctcgcactt
     6841 gaggcggacg ggctctttcc agcaggggtg gaccgggccg agcggtatgg atacgtcgac
     6901 ggtcttcttc atggctgttt ctcctcgtag tccttgaata ttatcggcgc gaccgagagt
     6961 atggcattga ggatctccgt cgggcggggc gggcagcccg gcaccgatgc cgcgatgggg
     7021 atgtaattct cgacgggcgg ggtggtcagg ctgcccctcc gggcatagac gcaccccgat
     7081 atcgggcagt tcccgatggc gacggccgcc ttcggctccg ggatcttatc ccagatctcg
     7141 cggagtttgt ccacccactg gggcgttacg gagccgatga ccaggaggac gtcggcctcc
     7201 cgggggttgt tgtggacgta gatgccatac tgctcgatgt cgtagcgcgg cgcaagacag
     7261 gcaagcacct cgatgtcgca gccgttgcac gacccgacat cgacgtaagc gacgtggatc
     7321 gacctcgacc ggacgaagtt cttgatctcc tgcaggatgt tcctcatgag aggaccgcct
     7381 cccggatccg tgcctttcct ctcgcgagcg cctcttcttg tggtccgttc ttgctcattg
     7441 ctcttgtctc gctgcatatc gactgcataa cacccgggtc cacgagcggc acgaaccggg
     7501 tgaagagttc gcacccgagt tctttcgtca cggggtcgcc gttgtctgcg tgctgctgcc
     7561 ccatctccca ccgggacccg aggttctcga gcagggacat gtcgaaccgc cggatcagca
     7621 ccatccggag tttcgcgtcg gagatgccga ggcgcccggc gatcgcccga acggcctcgt
     7681 cgtgcctcgt gctcgtgagg atggtgaact tcatctggcg gaggtctttc tcgtagaggt
     7741 acccggtcat acgtatcccc ctgcttttgc aagggcgtag ccggcgaaga gggcgacggc
     7801 cagccagagc cccgccgtct cgaacgtttt caaccgttcc tcgttcgagg cgtggtggac
     7861 gagggagagg gccacgagga ccccggcgag aagcaccgcc tcgccctgga tggtctggtt
     7921 ctgcagccac tgcaccaggg ctatgacgag gtacgccgcc cctccgagca gcaccagttc
     7981 gcggatcatg gcatcaccca ccacaggtat ccgacgtagg cgagcatcag cccggtgatg
     8041 agcccctgga tggtgacgct gtcaaacggc gagagcatgg gggataccgc gcagacgaac
     8101 gagagcgata cgagaacgat cgtcatgagg acgagcgccg cccagaacgg catggctccg
     8161 atgaagagag cgacgaacgc gaagaggagc acgaatgtct tgagcccgtc gatgacggag
     8221 atccctgccc gccagacccc gaaatgctcg gtcttgtagc cgctcacgag ttccttgctc
     8281 tcgacgatcg agaaggggcc gtagggcatc ttcgagagga taacgatgta catggctatc
     8341 gcgacaggcg gtgctatgag gaggaacggc ccgtggactg cctggtaggc ggtgatatcc
     8401 gaaagaagga gcgactccgt gaagaggtag atcgcggcga tcgtgacgaa gagcgggatc
     8461 tcggaggccg cggatatgac cgaccggacg ccgccgaact tcccgtaggg cgagcccgac
     8521 gagagcccga acccgtgctc gacgacctta tggagcatgt agatcgcaaa gagaatcagg
     8581 atgctctcgc cggcgaggac gacgaagagc gccgcgctcc agatgccgat gccgacgagc
     8641 acgaccccta cgtagagcgt agcgcttgct gtcttcggca cccaggtgct cttgaacgag
     8701 aacttcaggg catggaggat ctcctgccag acgggagggc ccggcctcat ctgtacccgt
     8761 gcgatgactt tccggtggag ccccagcagg aggagtccga acactgctgc aaataccagg
     8821 tactcgatca tgtcagtatc ctatgaacgg gatatcctct tctccgcccg gagcccgcca
     8881 ccagagcccc aggaagatga ccagtgccgg gaaggcgatc acgatggctg tctccgccgc
     8941 ccagagcggt atgaggcctg ctgcgagcag ccccgatgcg gcggcgatga cgatggccga
     9001 tgccgcaagt ccgtaggtaa tcgtcgttcg tttcatgtca atcagatggt tatggcaatc
     9061 tccacgacgc gcaggaagat caacagcgat gagagggagc acatgatgac gtagggggcg
     9121 cccggcgccc ggaacatctc ggcctttgcc gcgtagaacg gcgccatccc ctctccgatg
     9181 acgccgaggg tgaggagggc tttggccacc agcgggacag caaagccgcc ggccgcaaga
     9241 gccggtatcg agagcgtgcc ggtggtcgcg aggatcaacg cggcgccccc gaaaagcggc
     9301 agggtggcga ccatggcgac gatgccgtac tcgtacgcgg cgttgaggac gtgatggctt
     9361 ttgaccgccg cgacgatgcc gatgttcgtg atcccgacga gggaggcaaa gagcgtgaag
     9421 ttgaagaggt ccccgctccc catcgccccc atcgtcgcga ggccgcaggc gatcgccatg
     9481 aaccgcctga agcgcatctc ggagaccgtc gtctcctttg cgtagtaggc gtccccccca
     9541 aacgctatat cgatctgccc ctcgggcctg cttgcgatag caagggccgt gaagatcagg
     9601 gcgaacagga agagcgtcgt ggtgtagggg gtgtagtagt ggaggatatc cccgaactcc
     9661 agcacgaaaa ggtcgcttcc gccgatatca ggcattcgga ccaccccgca tggtgccgat
     9721 gagcgacatc ttggccatga ccttgacgag gatcgccgat cctgcgagca tgaccgcgaa
     9781 gaaccagtac tgcgggagga tcatgaagac gaagaacgcg acgatccaga gcacccacga
     9841 gtagccgctc gccgtctcga tcagttcgaa ccgctcgtgg tggttgcggc agaggaagta
     9901 gaagatcacg ccgaggccgg cgacggcccc gccggtgaac ccggagagga cgatgccgta
     9961 gacgacgagg gccgcggcga ggatgccggg agcactctcc atcacctcga tctgcaggcc
    10021 ggcatcgggt ggccggacgt agccttcctt tacccggtag atctcggaga gcgcgatcag
    10081 ctccgagacc cccaccacga ggcccgggag gatgagggcc tccgcgaggt cggtgccgac
    10141 gagcgcgatg aacgcgaggg tgacgacctc gatgaggtcc gtgagcagca ggcggtggag
    10201 gccgtcggtc tcccgcatca gggcaaagaa cgcgatcccc gccgacccga acgccacgag
    10261 gaggccgacc gagtaatcag ggatcattct tcctccctcc ggaagaggta tgaggcgatc
    10321 gcaaacgctg taaagaggat ggtcgtctcg acgaccgtat cgagcccccg ggtgttgtag
    10381 aggatctcat cgacgatccc tcccggatgg gcgacgatcg tcgtgccgag gtagtgcgtg
    10441 tggtccgcga ggtactgcga gagcggcgtt aagtaggcag tgacgtagcc cgcgtagggt
    10501 gagttctccg ggtactgggc gacgacctcc gttgtgccga acgggacgcc tccccggtcg
    10561 taggggtaga gcgaacttgc cgggtcgagg gtcttggggt agagctggtc ctccgcaaag
    10621 ccgagcggca gggagaggat gcccgcgacg gagacgatga tgcacgcagc ggcaaagagc
    10681 gcaacgaggt tctggaagtt ggagacgaac cttgagatcc gcgtgatgat cagcacgatg
    10741 catccctcca ttccatcagg cgcacgatga cgaacgtgct gattgcggtg acggcaacaa
    10801 acgtcagtat ggccagggcc tcgtcgtagg cgagcatgac gagtaagagg ccccatgccg
    10861 gtatctcgag gttgatcagc cgggcgacgt aggtcttggg gcgggtgaag gcgaccgaga
    10921 cgacgccgat cacgaggatg gcgagaccga ggatgaagac agcgttcacg cttcagccct
    10981 ccggacagcc atgaggatga atatcgtggc gatcggcgcg atgagtgaga ccgtgatggc
    11041 aacgtctaag tagccgcggt ccacgataaa cggcaggatc ccggcaacaa ggaccccgag
    11101 cgagatcact ttcccgtagg agtccctgat gagggcggtg gaggccgcgc ccaggagcgc
    11161 gacgagcccg aacgcgacct ctaacgcgac gatcaccgct tcacccctcc tgcataggtg
    11221 cttgctacgg cgtttgcctc gagcgtggag ccgacgaagt aggctgccgc cgcgacgaga
    11281 gagagcgggt ggtcgagcag caggacgatt gtcccggcga cggcgaagtt caggacgttc
    11341 gcgtagggga gtttccggcc ggtgttccgc tcgatgacga cccgtatagc ggcaaagacg
    11401 gctactcccg ccacaatata gagcgcgatc actgtctgac cctccagagc cgcgcaagaa
    11461 gcctgcttgc gtgggcatgc gagagtccct ggatggtgag gatggcgatc accatgcctg
    11521 cgatgaagtc cgtggcggcg aacccgacca gggtggaggc gtagacgaca aacgtggcga
    11581 ggaccgcgcc ggtggtccct gcgtagccgg ggtcatggca gatgcggttg ccgatgaaga
    11641 cgagcaccgc cgcggcgagc ccgccgggga tgccggcgac gtagacgccg caggcggcaa
    11701 ggagcgtccc ggccgaggcg tcgggagagc agacgatgtt ccccatcatg gtgccgccgg
    11761 caagagcgcc gccgccggcc tcgatatccc gcgcgatagc cgatgctccg ctgacacccc
    11821 ccgcttccgg caaccggaag aagatgtcga cggtcacgaa gagtatccag ctgatgatcg
    11881 ctgccgccag gatatggagg atctcgccca gcatttcagt tcaactaaag gaaaatacag
    11941 tcgatcaatt caataaaggt tttgttagta acattttggg cacgatccgg ccggaatgcg
    12001 ccgtcggcgc atcccggaca tgatactccc cggttaattt tgtatataat gtaatgcatt
    12061 ttatatacaa tatatgtttc cacgtgtctc tggggcccca ttgcgcgctg ttattgccag
    12121 attccgggcc gcaggatcgg gcacctgagc cggctggaat gcaggtgatg ccgcggtgac
    12181 gtggataccc tgcggtgcgc cccggccggg gcatcgggcc gggatgggac ctccgcctgc
    12241 ggcgccgccg gggaacaaat ataatgcttc ccgcgccgac ctatatggca cgcgagggtt
    12301 gccgagccag gtcaaaggcg acggatttag ggtccgttct tgaaggagtt caagggttcg
    12361 aatcccttcc ctcgcatact aacaaatttt ccatgagatt cctccaggtc agggcgagga
    12421 ttccctggcg ctctgctcca tcgtgcgaca ggcacggccg tcctttgggg agcctcaccc
    12481 tatcaggttt tcaacctctc gcagtgcgag ggtatcgagg tcgaacaagc cggggaggtc
    12541 cggccggtca ccacctgcga ggacctcatc gcccgcaacc ggccccgggt cggcccgggc
    12601 gagccggc
//
LOCUS       DAJB01000185            6433 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_86514, whole genome shotgun sequence.
ACCESSION   DAJB01000185 DAJB01000000
VERSION     DAJB01000185.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_86514"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1208 a         1803 c         1780 g         1642 t
ORIGIN      
        1 ggcgacccgc tcctctctct ccccggatcc gcgctggcaa tggctgttcg tggtgtctgg
       61 agtggagatg atgctctttg tcccaccgat cgacaccacg atgctcactt atatacaatg
      121 atttgtatat tcatcttaaa attctacgga cgcaaatggg tagatataaa tattgatctg
      181 gacaaaaaac acttgctcac gagggaatca atcgatgtcg acaaaggacc gtattaaaga
      241 aaaatatagt gatacccagc gaaagatcct gtatcatctc aagtccgggc taaaaacagg
      301 caaatcctac ttcaaatcca agtatattgc aactgatctg ggcctttctc cgaaagaggt
      361 cggcatcaac cttgctatcc tctcggagat ctgtgatgag ctggacatca ggcgctggag
      421 ttactcgaac agcacgacgt ggcgtgtcac ctcccgtgca tcataatttt attccgggac
      481 gctctattct acggcatgaa gatcattgag ttcgaagcac ctgtcgagtt tgtggccacc
      541 atcgacgacc acaaagactg cctcatgtcc caggacaaga gccatgagaa tcctgatgtg
      601 ctctggttca acatcgatgt ccctaaaggc cacggtgtac gggccggaga tcgggtcaga
      661 gtcaccgtcg aaaagatctg aggttagagg tacccgggga gcgtctccag ggtaatgcgg
      721 tatgaatcga cctcactcac gctgacgatg aggtagaaat cctttcctcc cctctcgacg
      781 tttttgatga tgctgcccgg atagtgcagg tcggcccctt ccacgatggt cccgctctcc
      841 agctccacga gcgccatccg gaagtgtgcc gcctcgggcc ttactgttgc agagacactg
      901 caattaagcc tccataccgc gtacggcacg tggaatacct cgctgatgcc tcctcgcccc
      961 tcctcgatgt acgcgaacgg gatgcgatca gcagttccct tccagggcgg gtcggaaact
     1021 ccgtagagcg tcaggttgtc ggggaggtga cagcaggggt aattccagac ctgggtcaca
     1081 taggagagcc tcgcgggttc gagcggcgcg ggcgatgccg gtacgggggt agcggtgacg
     1141 gtaggcgtcg gggtgagact ttccggaatc tcaggcccgg cctccgctac gggcggtcgg
     1201 aataccagtg ctactacgga gacaacgatc agggcggcaa gaatgctgaa gacgtcgcct
     1261 ttatccacag gtttagaggg atgtatcagg taaaaagacc ttcgggtttg actctctgct
     1321 tttgcccgca gagagtcgaa gatcgaggtt cgagagggaa gatatccctg ttttaccctg
     1381 cctcctctct ttgttgagat tataaagcgg aactgttaat acggagacgt gagtatcgtc
     1441 ctgatgagag catggaaatc actgaggttc gcacatgggt gatcttttat ctgctctcgt
     1501 tcgtggtgct ccttgcagcg accttcatca caacccagaa cgacagccag ctctggatca
     1561 gcctcatgct catcatcatc gtcgtcggtg taaacttttt cctgctcttc tccgagttga
     1621 agcgctacca ggcccggaag gccttcatgc accggatgtc gaagtttgac gacaccgaga
     1681 cggagaagga ctcttgcggc aaccgctggt gaccactcct acgcagcctt gcgcagaatc
     1741 ttttgcatga cgttaaacgg tgtaaagagg tgctccacct ctcttgccag gtactcggtc
     1801 ttgcctatga catagtaacc gtcgacggca agtgcagagt aaaaaaggtg cgtaaggtcg
     1861 ttcttctgct tctcggtgaa gtagatggtg acgtttctgc aggtgacgac gtcgaggtag
     1921 cgtgagatgg gaacacccga catcaggtcg tgctgccgga acttgacgag gcccttgata
     1981 tggggacgga cctcgaacgt gccgtcgtcg tggctgataa agtgcttgcg gatcttcttc
     2041 tcgtccacat tctcgagcgc cttcctgtca tagaccccgg ccgcggcttt ctgcagcact
     2101 acggcgtcga tatcggtcgc atatatcgtt acggtgacgt ctttatggag tgccatcagt
     2161 tcatgcacga ggatggcgat cgagtagggt tcttcacccg tggcgcagcc ggcgcaccag
     2221 atccggatcg accccctgcg acggaccaga tccggcagta tctcccgtcg tatcgcttca
     2281 aagacttccg gatcgcggaa aaatttggtg acgttgatgg tgagagcgtt tctgagcaga
     2341 tcgagttcct gcgggttcgc gagaaggtac ttatggtaag cctcgaaatc tgcggtgtcc
     2401 gtcaggcgca tccgggagag gatgcgccgt ttgatgtagt cctccttgta gttcgagcac
     2461 ttgatccgga ccagtttttc gatgctcttc tggagcgaca taaatgcatc catggggtaa
     2521 tctactctca ccgggtgttg atttacggag cgttctgata gcatccgagg tcgctgatca
     2581 ctttctggac gtcgagccag atgataaggg tctttgttct ctcgtcttct ccgtccccta
     2641 tcttgatgat tgctttgaca taattgctga tagaagacgt gatgccgtca ttgatacgtt
     2701 cgatctgctc gttacggatt gaaatgacac tgtgaacgtc atcgacgatg atccctacat
     2761 tcgacccgct cgccacatcc ggcatcagca cgacgatctt tttgttctcg gcctctccgg
     2821 attcagggag cccgaggagg tctcggaggt cgataatgtt tgttatctcg cctcgcaggt
     2881 tgatgatacc cgcaaggtat tcgggagcac ggggaagggg agtgatcggc atcatctcca
     2941 cgatctctcg tgctatctgg atatccagtg cgtagtgctc cttcccgagc tggaactcta
     3001 ccacgtctat ggatgctgcc atcttctcct cctgttagtt aatcttgaac tgtttcatca
     3061 ctgactggag ctgttcggcc atacctgcca gttcgtgcga cgcactcccg acttcctcgg
     3121 ttgatgcact gacctcttct gcgagtgccg ccatatcctc ggtccggtca aggctctcct
     3181 tggtcatgtg ggtggactgg tccatcttct gcatgacatt gttggtggag cccgcctggt
     3241 cttcggtggc cttcgtgatc tcggtgatac cgtgggcgac tacttcgacg ccgttgacga
     3301 tccggtttaa gccctcgatg actttgttga cgctctcaat gcctgcctgg atctcctggt
     3361 gggacgcctg catcgagcct gcggtcttgt cggtgctcgc ctggatcgtc cggatcaggt
     3421 cctcgatatc ctggctcgcc cgcttggact ccccggcaag gtttctgatc tcgcctgcaa
     3481 ccacggcaaa cccgcggccg tgctccccgg cgcgggcggc ctcgattgct gcgttcagcg
     3541 caaggaggtt ggtctgattg gcgatatcgg tgatcagctt gacgatgttg ttgatctccc
     3601 tcatctggct gttgagggtg ctgatctcgt ccacggtctg tttggagatc tcctcgacga
     3661 gctgcatctt cttcgctgcg acttcaccga gagaggctgc attgtctccc tccttcgacg
     3721 ccttcgaggc ctgttccatg acctcttgcg acgtgcttgc gatctcctcg atggacgccg
     3781 agagatcgtt gatatccgtt ccgatctcct cgatcttatc gagctggcgc cgtgcatcct
     3841 ccgacgactg ctgcgtcgag gtggcgacct gctccgtggc ccggatgatc tcgtcggtac
     3901 tcttgctgac ctcttttgtg gtcatatcta cccgatgggc cgcttttgcc acgtccgcga
     3961 ggagtgcgcg gatggcggtc agcgaggcgt tgtaatcgct cttgacttca cggagagggt
     4021 cgcctgcctc tgcgtccacg aatgccgtca ggtcgccctt tgccatggcg gcgagtgcgg
     4081 tcccgagttc ccgggcgctc tctgcgagtc tctcgctctc tttctttgcg ctctccatca
     4141 gctcccggat ctggtcctct ctctcccgct gctcggtgac gtcgttgtag acgttcagga
     4201 tccctgtcag ttcgttcttc tcgtttaaga gcgggatccc gtactgttcc atgatgtagg
     4261 tgccggaggg gaactcaacc gtcacttcgc cgaaaacccg caccttctcg gtcagcagct
     4321 tcttgatccc ttctcctttc tggttgtgaa ctttgaagtc ccgcagggtc atcgtggtga
     4381 tgcgcgaccg atcgatcccg gtcaacttga gatatgcctc gttggccgtg aggatcttca
     4441 tccccgtatc gacgaccagc atgggcatcg ggttctcctg gatgatcgtc tcggactgcc
     4501 gttgcagttc ggcaatattc tccatctgga ccttgatctc ttcctctccc tcccgctgtt
     4561 tggtgagatc gttgtagacg atgaggacgc tctcgaccgc gttttccttg tcaagaagcg
     4621 gaatgccata ctgctcgagc gtatgggtgc ccgagggcag ttcgacggtg acttccgcat
     4681 acgcacgctt cttctcctgg atcgcctgcc ggaggccgga acctttctgc gacgttattt
     4741 tgaagtctct gaggctcctg ccgagcatct ggtccatcgg gatcccgctc atctcggcat
     4801 aagcagcgtt tgccacagtt actttgaact gcggatcgac gaccagcatg ggcatcgggt
     4861 tctgctcgat gatcatatcg gatcccttca gaagcgctct ggcctcttca agccggcgcc
     4921 tgagggcctg cttctcctcc tgcttcttct caagcagcgc gttgatcgat gctgccagag
     4981 gccggaattc ctcctgaatg ccggcaaggt cgatctttgc ggaactgtcg ccgtcaagcc
     5041 ccgcatggag tgcagcctcg atcgcctcgg ggagtaccac cggtccctgt ttctgtgcct
     5101 gtccagtcgt tcccggcaca atcggtccgg taaatagtgc tttccttcca ggtttatcga
     5161 tcattaatac acatcctcat catgtttctc gtctcgttcc agaagtgcag ttgctattgc
     5221 ccggtattcc ctgccggcgt cactctcggg tgcgtaatgg gagatgggaa gcccccgttg
     5281 ctgtgtctcg tagattgcag gcgagtaggg gacggtgaat acctgcttga acgctttttt
     5341 gacctcggac tcaaacgcct tcagccgcat cctctccttc tcctcttctg cggaggggcc
     5401 ggctgaaaag attcgtttca ggaagagtgc aaggccgccc gttccggccg ccgggtcgcc
     5461 cgatcccttc cagcgggtca gaatggcgaa atctgcggtg acgtcttccc cgagcatctc
     5521 tctgatgtcg ccaaagactg ttgacagtgc ttccatgcct ctgagggcga aggtgcccgg
     5581 gtcgagggtg acgatggtat ggtccgctgc gacgaggccg ttgatgacga actggcccat
     5641 gctcgggggc gtatcgatca ggatgaagtc gtaggcatcc ctcaccgggg tgagcgcctc
     5701 cctgagcagc cctgcccggt cctcgatacc gtagaggtag ggctcaaccc ccacaaggtc
     5761 cagggttgcc ggagcaaggt cgactcctgc tgccgtgggg acgatcacat catttattgc
     5821 gatgtcggga aagccctcaa aggcattcat aaagacgtca tacatgctca gttcgagggt
     5881 ttcggggttg accccgagcc ccgtggtggc attggcctgc ggatcgcagt ctactaccag
     5941 aacgtgtttt ccgtctttct ggagatatcc tgcaatgttg aggcacgacg tcgtctttcc
     6001 tgtgccacct ttatggtggg cgaacgctac gattgtaata tccagcaccc ctcataccac
     6061 tatgtgaaat gcgggttatt aaacctattt ttatgatgct gtgcagatgt gggatgtgtt
     6121 ccacatttaa aatactacag cacccttgat gagaaaattc tgcctgtatc tgctattgct
     6181 tatctccggg atcagagggg cgggtacaga aggtcttact tgccgttcgc cgtcgtcccg
     6241 ggcttctgcc tgtgagtcaa ctacccccgg ttaaaaccgg gggcttgcag ctagaatcat
     6301 cggagatgct tctgctacca taggctggtt ggcggcagcc ctgaaggcaa tgtttttcgc
     6361 agcatttgtg tcagcaggtt cggtgtggcc gcacgcccta caccaaaaga ggtttctggt
     6421 tttccggttt ttc
//
LOCUS       DAJB01000186           11968 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_15017, whole genome shotgun sequence.
ACCESSION   DAJB01000186 DAJB01000000
VERSION     DAJB01000186.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_15017"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2402 a         3688 c         3713 g         2165 t
ORIGIN      
        1 agaagcgtgt aaaaaacatc ctcccagcac ctcccccgca cggcacggcc gcggtggacc
       61 gcgacgatgc agtccccctc gatgccgatg cggatgcacc cgagcgggtc gtaggtgatc
      121 tcgtccgggg gggaatacgc gtccatgaca ctcccgtatt ccagcggggg ttcgcgccgc
      181 cgccgtttct ctttcaggat aaggaggtcg agccccagat ctttgggata ggggcggtcg
      241 gtcatcagcg ccatcatctc ggccgcccgc cgcatctccg ccaccgatcc ccgggtcttg
      301 tcgctgtgtt cgctcgtgaa gacgacggcg gccccgacct cgtgcgccat ccccgcgagc
      361 agcgcgttcg cccccggtga gtcggcatcc agcagttcca cgacgttgcc cgccccgaag
      421 aagagcgggc gggggacctc cccaaacccg gcaagcgatc ccgtcagccc ggaccccacc
      481 ggctgaagga ggggatcggc gatcaggcag gagatcccgg cttcttgtgc ggcggcaagg
      541 ttctcggtga gcgtccggtc acggggcacc acgaccgcgg ccgccccggc ttccgcgacc
      601 gcctccccga cgaccgggat gtttccctca tgcagggaga gcaccaggtc ggcacggaag
      661 agcgccgccg cgatgaggga cgggtcctgg gtatcgaccg ccagagggcc gccaacctcc
      721 gcgacggccg caaaacaccg ttcgacgtcg ccgggggtcg cgtcgaaccc gaatcccagg
      781 tccacgatgt ccgccccgtc ggcaaagaac ccccggacat cggtgaggag atcgtcgcgc
      841 ttatgcgcgt ccatgatctc cgcgagcacc ttcatcctcg aacccccgcc gatctttgtg
      901 ccccggatga tgaagtccgc ctccgcctcc gcttcccgga gcgccacctg ccggcaggcc
      961 tcctctcgac gcacctgtgc gagaaattca tcggcgggaa cagttgtcga aagccggacg
     1021 cgatcgagca ccgaaaggac cagcgggaga tcggccgcat gccgcgggcc ccggtagacc
     1081 gggatgccgg tcttgcgttc gacgccggca aaggatgcgg tgcacatccc ggagacgatc
     1141 gccatatcgt agctgccgcc ggcaagcagc ctctggaggt caccgggggc aagaaacgac
     1201 gcgatatcgc cggtgaccgc cacgtctatc tcatagcggt cgctgaaccg ctcagccgcc
     1261 gccttcacga ttccgaccgt cgccgatccg gtcggcagga gaatgcgcat acacttcact
     1321 tattattcag aggaggacaa ataatctgat ctcatgctgc ggtgcgatct gcatgtccac
     1381 accaggtact ccagggacgg agagagtagt gtggaggata tcctccggcg ggcggaggcg
     1441 gtcggcctcg acgccgtcgc gatcaccgat cacgatacgg tggaaggtgc cctctacgcc
     1501 ctggagtgcg acacctccgt gaccgtgatc ccgggcaccg aggtatcgac aagacagggc
     1561 cacctgcttg ctctcggggt cacggagccc ctgccggcag ggcttgactt cttcgagacc
     1621 gtcgccctgg cccatgcccg ggatgctctg ctgatcctgc cgcaccccta ccaccgctgg
     1681 cgccacggcg tcggaaggag actcgcggcc ggtatcggag cggtggatgc ggtggaagtc
     1741 ttcaacagcc gttacatcac cgggtcggcg aaccggaagg ccgcagtcat tgcacggaag
     1801 ttcgggaaac ccggcgttgc cgggagcgac gcccacaacg cccgctacgt cgggttcggg
     1861 gtcacctatg tcacggccga gccggatgtg gcttccatcc tctccgcgat ccgggaaggg
     1921 cggacgatgg cgggggggag gatgaccccg ctccacacct acacccgcca gtccatcaag
     1981 ggcgctctcc gcagaatacg gcggacggta taccgatgaa tctggcgttc cgctattcat
     2041 actttggaga ccgtttcttc ggctcgcaga tgcagccgct cctccgcacc gtagagggcg
     2101 aactcgtcgc ggcctgcagg cgcctctgcc ttttcgacga ctggcgggag gcaaacttcg
     2161 tcaccgccgg ccgcaccgat cgcggggtgc atgcccgggg ccaggtctgc tcgtttctga
     2221 ccgataagca ggatcgggcg gtcgaagcac tgaaccaggt gcttccggcc gacatatggt
     2281 gtacggcgtg ggccagtgtt ccggacgggt tccacccccg gtacaacgcc gtatcgagga
     2341 cctaccgcta ctacttcctg gccccgggga acactgtcgc catgcatgaa gcagcacagg
     2401 agtttctcgg gcggcacgac ttctctacgt tcgcccgtgt cggcgaccgg aatcctgaac
     2461 ggaagatcct tgcggcacgg gtctttgagg acgggccgtt tgccatcttc gaggtgacgg
     2521 gagagagctt cctctggaat atggtccggt gcatggcgac agcacttgaa cgggtgggaa
     2581 ggggcgaggc cgatgccggg gagatcgcac ggctccttgc cggaccgccg gagcagaggg
     2641 tccctgcggc tcctcccgag gggctggtcc tctgggatat cggctatgag atctccttta
     2701 cgccgctccc gatcggcgag aagagtcgcc ggtaccttgc cgaccgccat cgccaccacg
     2761 ctctcatggc ggagatctct gcacgccttg caccggaggg ccggtgcccc gacacatccc
     2821 caatataata cctcgcaata cggcaaaagg gtgcagagag gatcgggaaa cagacccctc
     2881 tcattaccgc tgctttaaaa ggatcagacc gcaggcaacg gatcttccaa atgcggtgcg
     2941 gcaggggaaa tcggactctc ccccctcatc cggggctgct gcacttaatc tttccggata
     3001 gcgatacccg acaaaccaga accctgccga aatcccggca tggaggtact tcatccgtcc
     3061 ctgcgacagg tacgtagact tccgtccagg agccggaacg cttaagtatc ccgggacagt
     3121 tttaggcaca cctgagtaaa acagattaga tttattttca atatagttaa acgttttagt
     3181 ttaataataa cctattaata tatgacagca cagtcccgtt tcagtacgtc aagaggggaa
     3241 agtggatgta cgacaaggat ccggttcggg cgaggccatc agctcatcta gaggggtggt
     3301 tcgccgaaga aacaactgtg ccattaccgc agtatacaga tgcagagaga gaccgatgga
     3361 tagaacgaca tataccccgg agtatcggcg aggagagccc aataaacatc cctgccctcc
     3421 acgttgaggt ccaggagcag gccaaccctt tgcgggcgcc gacggccccc ggacaccggc
     3481 gggtggtggg cgagaccgct gatgctgccg ccgtcccgcc ccgccggtcg agcaggacga
     3541 gaacacttgt ggcgatcccc tgtttcaacg aggaggtcgc tatcggttcg gtcgtcctcc
     3601 gggcccggca gtgtgccgac gaggtcctgg tgatagatga cgggtgcacg gacagcaccg
     3661 tgaaggttgc ccgggatgcc ggagcgaccg tcatctcgca cggggcccgg aaggggaagg
     3721 gacaggggat caagagcgcc ctccggtatg ctgtcgacca caactacaac tgtctggtct
     3781 tcatggacgg agacggtcag cacaatcccg aggagatacc ccttctggtc gagccaatcc
     3841 tcgccgatac cgccgacctc gtcgtcggct ttcggacctt cgaccagatg cccttttacc
     3901 ggaggttcgg ccgggcggtc cttgacgtcg ccaccaacac cggcagttcg ataaccgatt
     3961 cacagtgcgg ctttagagcg ttgaacagga agtccatgga gtcgatgctc ggtacgctcc
     4021 gaaaggatga tttttcaacc gaatcggaga tgctccggat agcacaggag aagcatctgc
     4081 gtgtcgggga gacaccgatc aactgtaaat acggtgattt tgccacatcc acaaaaaacc
     4141 ccttatctca cggaatggag gtgctcggat cgatattctg gctcgtggtg gagaggaggc
     4201 ccctcctgca catcggcctg cccggacttg ccgcgatgct tgtgggcgtc tttctcctgc
     4261 tccagtttct ccggggattt agtgagaccg gtctcgtccc gatcgagcag ggcatgcttg
     4321 catcgctctt cctgatcccg gggaccatcg cactcgtgct gggcctgatg cttgcccttg
     4381 ttgcaagaat gcgggagtga gcaggagtga aaggtacgat gcagaggaga ggtgtagaat
     4441 gatcggagtg atactcggca cgcggccgga gatcatcaag atgtctcccg tcatcaggga
     4501 atgcaagcgc aggggtcttg actattttat ccttcataca gggcagcact actcctacga
     4561 gatggaccgg ctcttctttg aggaacttga gctcccggag ccgaactaca gcctcgatgt
     4621 aggttccggg acgcatgccg gtcagacagc gaagatcatg accggcgtcg aggatgtcct
     4681 ccagaaggag tcgcccgaca tcgtcctcgt acagggggac acgaacaccg tcatggccgg
     4741 ggctctcgcg gcatcgaaac tgcatgtgcg ggtggggcac atcgaggcgg gcctccggag
     4801 tttcaaccgc tggatgccgg aagagatcaa ccgggtgctc accgaccata tctcggatta
     4861 cctcttcgct ccgacggaga gcgcccggaa gaacctcctc gcggagggca ttcccgagca
     4921 gaagatctgc gtgaccggca acaccgtcgt cgatgcaata taccagaacc tcgacatatc
     4981 gaggaagaaa ggcaacgtcc taaaagacct cgacctcgag cccagtgaat acttcctcgt
     5041 caccgcccac cggcaggaga acgtggacaa ccggacacgg ctcaaggaga tcctgaaagg
     5101 gcttgagaga gtgcagaagg agttctccct gccggtcgtc ttcccggttc atccgaggac
     5161 cgagaagagg atcaaggaac tggggatcgg cgtcgacgga ttaaacctca ccaaaccctt
     5221 cgggttcctg gagttcctgc agctggagtc gcaggcaagg ctcgtactga ccgattccgg
     5281 cggcgtccag gaggagacct gcgttctcgg tgtcccctgt gctacgatgc gttacgacac
     5341 ggaacggccc gagacgctgg acgtcggatc gaacatcctg gtaggtgccg actccgggag
     5401 gatcctcgaa ggagtccgct ctgcagccac gtggagacgc ggctgggcga acccctacgg
     5461 ggatggaatt gctggaaaga tgattatcat ggtctgtgca gccgcacggt cccaataact
     5521 gcacgaggaa atacacatga agatctgtgt actgggatta ggatacattg gattgccgac
     5581 cgcgctgctg tttgccgctc acggggcgga tgttgtcggc gtcgatgtga agcagagcgt
     5641 ggtggattgt ctgaatcatg ggaacctgcc gttcagggag ccggggctcg atgacctgta
     5701 ctgtgatgca aaagagcggt tttgcgccag gaccgaaccg gaggccgcgg acgtattcct
     5761 gattgccgtg ccgacacccc tggatccggc gacaaaggtc tccgaccttt catatgtcaa
     5821 gaaagcggcc gagatgatcg cctcccatct cggcaggggg aacctggtta tcctcgaatc
     5881 gaccgtcccg cccgggacga gtgaacgggt cgtcatcccc cggcttgaga agagcggcgt
     5941 cgttgccgga gacttcctgt acgcccattg tcccgagagg gcaatcccgg ggcggaccct
     6001 gcaggagatg gcgggcaaca gccgcatcgt cggcgggtac gaccgggact ccaccgaccg
     6061 ggcgacctcg atctaccaga cctttgtccg ggggcagata taccagaccg atacgcggac
     6121 ggcggagttc gtcaagctga tggagaacac ctgccgcgac gtgaacatcg ctctcgcaaa
     6181 cgagttcgcc cagctcgcgg aggagtgcgg gatcaacgtc tgggaagcca tcaccctcgc
     6241 aaacaagcac ccccgggtca ccatcctcaa ccccggtccg ggcgttgggg gacactgcat
     6301 cgccatcgac ccctggttcc tgaccgagaa ctcaacacgg tgcaggatgg tctctacggc
     6361 gcgggaagtc aacgattcca tgccgaacta cgtgctgcac gttgtccgcg gcctgcttgc
     6421 cggcgtcaga aatccgacga tcagcatctt cggtgtcgcc tacaagggca atatcggcga
     6481 cacccgggag agcccggcgt tcaaattcat ccagctcgct gagaacgaag ggtatgccat
     6541 caggtgccac gacccgtatg cgggggagtt cccgcacccc ctcatggacg ctacggatgc
     6601 gacggccggg agcgactgtc tcgtcattct cacggatcac gactgcttcc gtacgatcga
     6661 accggcaggg cttcggatga gaacgaagtt cgtcatagac acccggaaca tcctcgacca
     6721 tgagaagtgg accgaccagg gttttgccgt ccgcgtgctg ggtgacggtg catcggtgca
     6781 gcagacacca ctggacggga tcgcaccccc ggcaaacctg tatcccacag atgctgcgcg
     6841 gtcgatcgca tccccatctc cgccgtccat ctccctgctc tctcccaacg ggcgggaggg
     6901 cttcctctaa cccccgcaca cggtttttac aggggaggaa gcaacaaccg gtctccggcg
     6961 aacagatcca gatactacga ggagttccat gaaacaggta tgcatcgtat cacaacattt
     7021 tccaccagaa aaatcgggaa acgcatcacg catctatgac accgccgtgc atcttgcgag
     7081 actgggtatc gacgtgaccg tgcttgcgcc gcaccccacg tttcccaccg gctcgttttc
     7141 ccggacctgg aagcgatcga atgtgcagga ggtcgacggg atccgggtag tacaactctg
     7201 gacctggcag ccgggctctg gaaatccggg atttgcaagc aggatggcct actacctcct
     7261 cttcccgctc cacgccgcac tctggctcct tgtcaaccgg agtcggttcg atgcgatcgt
     7321 cacgtcggca ccgcccctct tcaccgggat cccgggatac atcctgaaac ggacctcgcg
     7381 ggtgaagtgg atcctcgata tccgcgacct ctggatcgac gcatcgataa gccttggttt
     7441 cctccgggag gggagcgtct acgagaggat gagcaggagg ttcgagcaga tgtgcctcgc
     7501 ccgggccgat ctcgtcggcg tcacgacaga ggaactcggg cggaggatct cgtcgcgcta
     7561 ccgggtcacg gccccgatgg agttgatgcc gaacggtgtc aacaccgact tcttccgccc
     7621 caacaacggc ggaaagaagc ggcggatcat ctatgccggc aacgtcgggc atgcccagga
     7681 tctcgataag gtggctcttg ccgtcaagtc gatgaacggc acctacaacc tgaagttcct
     7741 catcgtcggc gacggcgaca cgagggagaa ccttgagagg ctggtgaaag ccgagagcct
     7801 caccggctcg gtcgtcttta caggcaccct tccgcgtgag gagatcccgc agttgctctc
     7861 cgagtcgctc gtgggagtgg cgcccttaaa gaggctcgaa aacctcgagt acgccgtccc
     7921 caccaaggcc tacgagtaca tggcatgcgg gatacccttt gtcggctgcg gatgcggcga
     7981 gatcgcccat ctggcgagag agtcgggtgc cggggtcatc gccgacaaca cgccggaggc
     8041 gatcgccgcg acgctctcgg cgctcctgga cgacccggag aagatggagg agatgggccg
     8101 ccggggccgg gagtacgttg cagagcacta cgaccggcga tcggtcgccc tgaaactgaa
     8161 gcagtatatc gagaggatga catggacggg cgcctgaaat cgcttctttc ccggatttcc
     8221 ggggaggtca gggagctcgc cgtcaacgac ggcgagaccg cgtacatccg cgacccggtc
     8281 tttggcatca tccggaaccg cgtgcacgcc gaggtctgca aagcgatgct ccgctcaggc
     8341 ggggacccgc tcgtgggccc gatcctgaac tatgtcgcgg catgccagaa cccggacggg
     8401 tcctacaacg agattcacgt caactacaac gagccatcgg ccctgatcac cgcgtttatc
     8461 ggcgacgcgc tcctggaggc ggcggaccgg tacccgcacg aagaggtact ccggaaggcc
     8521 cgggatttcg ttgtcgccgg cgaaagaagg ccggggtgct tcctgaagtc ctcggggtat
     8581 accgccgacc acctgaatgt cgatgcctcc tgcggggcgt tccttgcccg gtatgcggaa
     8641 cgctacgacg atgaggacgc ccgtgcggcg gcgctgcggg cggcggagaa catcgtccgc
     8701 caccagtgta aatgcggctt ctacccctac gccgtcgata aggggaccta cccgtacgtc
     8761 ttcaaactcc cctgcgtgca ttaccagggg gtgacgatgt actacctggc aaagataaac
     8821 gacgtactct gcgacgaccg gattgacgaa agcctcgcga agggtgcccg gtggctcgcg
     8881 gccgcccagc gtcccgacgg gcggttcgac tggtcgagga gcggcctctc cttcgcctac
     8941 tacctctccg gggcatacgc gttcgcccag gcttcctttg tctacgcctc ccggagggaa
     9001 gaccgttacc gggagcacgc cgacctctgc cttgattggc tcgataagaa cgtgcagggg
     9061 ctcgcccccc ggtgggagcc aggctcctgg atcgatctga tcccgtccgt cgccacggca
     9121 gcaaagacag cgtcgcgtgg gaagtatccg ctacggcacc gggcgttccg gttcggctac
     9181 ggcatgtacc ggcagatcgc ccgcaggcgt tacagcaaga ccgcggagac gcggtcgttc
     9241 gaggccctct gccggctgct cgggatccgg tcgtcgaccg tggaaccgtc gaagaacttc
     9301 cccgatctct tcatgacggc ggagatgctc gattgcctca gccagtcagc aatatggggg
     9361 gaatacgcat gaaacaggta ttactcgacc tgcagtcagg gtctatccag gtggagaacg
     9421 ttcccgttcc cgccgtaacg aggggtgtcg tcgttgagaa cgcttactcc ctcatcagcg
     9481 ccggcacgga gtcgtcgctg atcaatctcg cacagcagtc gctcgtcggc aaggcgaaag
     9541 cacgtcccga cgacgtgaag aaagtcctcc agaagatcgg tacggacggc cccctctcgg
     9601 cgtaccagca ggcgatgagc cgtcttgcaa agcccgaacc gctcggctac agctgtgcgg
     9661 gcacggtggt gacgacgggc tccgatgact tcgaggtcgg cgaccgtgtc gcctgcggcg
     9721 gggccgggca tgcagtgcat gccgaatatg tcgcggtccc caagaacctc tgcgtcagga
     9781 tccccgacgg cgtcagtttc agggaagcgg cgttcacgac cgtcgggtcg atcgcgatgc
     9841 acggtgtccg gaacgcccgg gtcacggtgg gcgagaacgt cgcggtgatc ggcctcgggc
     9901 tgatcggcct tctttccgtg cagatcctca aagccgcggg gtgccgggtc atcgggatcg
     9961 acatcgaccg ggagaaactt gcgctcgccg cagacctcgg cgccgacgtc gtctcgaact
    10021 acgacggcct ctccgagagg atgaaggcct tctcgccgtt cggggcggac gcggtcgtca
    10081 tcaccgcggc cacccggtcg agcgcccccg tcgaggccgc cggccgcctg gtcagggata
    10141 agggcagggt cgtggtcgtc gggaatgtag ggatggacct gccccgggag atcttctacg
    10201 agaaagaggc ggaggtcgtc gtctcccggt cctacggccc ggggaggtac gaccggaact
    10261 acgaagagag aggggttgac tatccgatct acgtccggtg gaccgagcgg aggaacatgg
    10321 aggcgttcct ggaactggtg cggcagaagc ggatcgacct cgaccgcctc atcacccaca
    10381 ccttcacgct cgacgacgcg ccggaggcat acaacctcat caacacggga aaggagcggt
    10441 ttatcggggt tctcctgcag tacagcccga acggttcagg tgctgccggc accgtcatcc
    10501 acctcccgga gccggagaag cgaaggcggg aggttccgac cgcagcccgg acgctcgggt
    10561 gtatcggtgc cggggtccac gcccagagcg ccctctaccc ccacctgccc ggactccccg
    10621 tgaacctcgc ggggcttgcg accgcgacgg gcctctcggc gcagacggtg gcgaagaagt
    10681 acggattctc ctactgcacc accgactacc gaaagatcct cgaagacacc gggatcgacg
    10741 cggtgatgat cgcgacccga aacgacctgc atgcccccat ggcaatcgac gcactgcgcg
    10801 ctggaaaaga cgtcttcgtc gagaaaccgc tcgccaccga catcgacggg ctcaaaaaaa
    10861 tcgttacagc ctggaacgag tccgggaaac agctggtggt cggattcaac cgtcgccact
    10921 ccccgcttgc acggaggatg aaggagtttt tcgggaaccg cgctacgccg atgatcctgc
    10981 actaccgtgt caacgccgga cagatccctc ccgaacactg ggtgcacgac gatgagcagg
    11041 gcggcggcat gctgatctcg gagtgctgcc acttcatcga cttcatgcag tatatcaccg
    11101 gggcacggcc ggtccaggtc tacgcccggg ccatcgagcc gacagggacc ctccggaagt
    11161 acgacaattt ccaggcgacc ctgacgtttg acgacgggtc gctcggcacc gtcacctaca
    11221 cgacgcttgg cgaccgatcc tacgccaagg agatggtcga ggtcttctgc gacaacgccg
    11281 tcgggaggat caccgacttc cgcgacctcg agctccggcg ggatggaaaa gcgttgcggg
    11341 agaggcggtg gctcacccag gagaaagggt ttgccgagga gctcctggcg ttcgtgaagg
    11401 gcgagaagcc ggactttgtc ggcaacgtcg cgacaaccct tgcgacgttt gcggcatggg
    11461 cgtcgatcga gaccggggca ccccgggaga tcgatctggg gcaggtcggg ctttgacacg
    11521 gttaaaccat ttttcgcgat atctacagac agtggaatac atcactggag agaggagcgg
    11581 ttgttggttg tgctgttcta gttcaaccgc acttcaagca cggtaattcg ggcctccaat
    11641 tgggtgttca cgggtatgag ttccactatg gttcgtttga ggtctttgat agtagttgag
    11701 agaacggggg atattcgcaa cgatatagtg ctacgacggg tcgctcgcac agttatttat
    11761 ggtaaggaga tattgctgga actcagtctt attccgatgc tctaataaaa gatcttttgt
    11821 ttaagattga acagctattt tttcctttac tgttttgctg cgcattcaat tccccaaaag
    11881 tccttgaaat acgtatcggc agctttttaa tattagtatt aaaatttccc tccatgataa
    11941 atgaagcctg aactcccgga tccttagg
//
LOCUS       DAJB01000187            6510 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_60453, whole genome shotgun sequence.
ACCESSION   DAJB01000187 DAJB01000000
VERSION     DAJB01000187.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_60453"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1313 a         1859 c         1823 g         1513 t
ORIGIN      
        1 tcgacgttct tgagcccgtg aaacttctcg gggagggggc agaccgactt tgtgaggagt
       61 tcgaaccggt cgacccagat ggtgatctcc cccatctttg tccgaaagac gtggccgacg
      121 acgccgatga tatccccggc atcgatgtat tgtttaaaga ggtcgaactg ctcctcgccg
      181 aggtcgttct tccggagata gagttgtatc ctgccgcccg agtcccccat gtcggcgaat
      241 atcgtcttgc cgtgctggcg gacaatgtag atgcggcccg cggtgctgac ttcttcctcg
      301 ctcttctcat gctcgattgt ggagaaccgt tccttgatct cctcgacggt atccctgcgc
      361 tcaaaggtat aggggtatac cgtcacccct cgatcgagca gttcctgtat cttatcgatc
      421 tttgtcgtat caaaactgag atgatccata tcgctcatag tgtaaacagc tccattcgtc
      481 ctgcagccgt gccccggttc ggaaatccgg gtcaggcgcc gctccggcga gaccgggcgg
      541 tgaagtcgcc gggaaattct atatagtgtt catctctggg ggtattaaat tggcgacnnc
      601 gacgggcggg agcgtagtgc gactggccga agggcaggag cttgagcacc gccaggtgcg
      661 accacgagcg gccggagttc gagccccgca catccaaaac agcccctcac tcgcccgatc
      721 tcttcacgtc ttcgaaccat tccgttccgt acgaggggta gaggtagaag atcgtcgttt
      781 cgttgtaggc catcatcacc cgctcgttgt ggtggtgatc gaagacatag tacccggaga
      841 accgatttgt ggacgtccag ttctggtggt agtcctcagg gtaagggcct gcatacaatc
      901 cccctctcca gaatgagacg ttgcaaaatc ctcttcccgt gtgcccctcc tgttcgtcca
      961 cttcgatgaa gaacgacggt gactgttcgt cgcgtcggaa ctctacatcg acatacgtcc
     1021 tcaagtcgta atcaaagttc gtccgaaccg aagttatctt ccagccgtcc tcaagcagag
     1081 caccggcccg cgggtcggcg aggaggatct cgatcacctc tgttgcgttg gcgccgaatc
     1141 cccgcactgg aatctcggag acgttgatcg ggatcgagac gtccccccgc tggtagtgga
     1201 ggaccggggc gggggtgacg gtttccgctc cattcgtctc cggggagtcg gtgcatccgg
     1261 ccccgaacgc caggagcagc agcgcaaggg agaggatgaa gatccgtttc accttattgc
     1321 tcctgccggc tgcaggtacg gcttgtgcct gccttcccgc tttttggttc attttgcttc
     1381 cctcctattg gtggttccat gatcgttccg gcaacctgta ttttcccgga tcgccctccc
     1441 ctacgctttc atatcgccct gttccgtgtg agaggggcgc cggttccgct tcgatcactc
     1501 ccattcagtt acatccccgt atgatgggta gaggtagaag atcgtcgtgt cgtcataggc
     1561 catcatcacc cgctcgttgt gatgatcgac gaccacaaac aggcccatat acgggtttac
     1621 tgcctggtga tagtcctcct cttcagggaa gggttccgat gtccacgggt gtcccctcca
     1681 gatcgtggcg ctgcaacgcc ctctgccggt ttgcctctcc cgttcgtcca cttcgatgaa
     1741 gaacgacagc gacagttcat cgttcctgaa ctccacgtcg atgtgctccc ggttcgggtc
     1801 ctcctcctca aagcccgtcc ggatcgaggt gatgttccac ccgccctcaa gcagaatccc
     1861 ggcccgcggg tcggcgagga cgatcccgat cacctctgtt gcgttgacat cgaaccttct
     1921 caccgggacc tcggaagtgt tgatcgggat cgaggtgttt ccctgctcgt agtggaggac
     1981 cggtgcgggg gtggcgtttt ccggtgtgct ggtgcatccg gccccgaatg ccaggagcag
     2041 cagcgcaaaa aggagaaagg aaatttgtct catcgttcat cagttgtagg tatggaggca
     2101 cacccgcgaa ggatcctgaa tgatctgcac ccagcagtat tgcatgatct cgtagggaac
     2161 ttcggtcgaa taccacggtt ccaggtcgat gctcatgggt gccccataac tcttgtacac
     2221 catgtcgtag aagagcgtat cgcccggcac gtcggtgttg tcgtcgatat tccagccttc
     2281 aagaacgcag ccgacacggt acccggcccc cggctgcatg cattgcgtcg acagcgaact
     2341 gtactggccc gttgtatggt cgtagatggt gatagaccag agattgagac tgctgctcca
     2401 gtaaatgcgc cctcgcatcg tatgcccggt ggcggtgccg aacaggggcc cataataact
     2461 atcctggtag ttgggactgt atgccgccca ggcctgccct tcccattgat gggtggatcc
     2521 gttgtaggcc agcaccggct gcaacaggta cgtcgttgaa ttgacagtcc cctcgatgcc
     2581 gttgaagaga tagatcctct cattaatcgc aagacccgaa ggcgggctgg acgggaccgt
     2641 ccagtatgcc gtgtactcgg tgatgttgga atttggtcta tcctctgccg attctaccca
     2701 gctattaccg gtccacatct tcggctccgc cacattctga ccggaggacg aggtctcatc
     2761 aatgacggtc aggatcagtt ctccggcagc atcaaggacg aaggtctgat ctccacggtg
     2821 gtataccctc gagtcgttcg ggagttgatg cagtcgggta caggattttt cgacaccagc
     2881 aggtgtgggt agttttccgg atttttcgtc actgatcgag agcaactggt tcccatcgtt
     2941 gtcgaatacc agggtgatcc cgtcggctgc gtggacgacg atcgaaccga acgggatccc
     3001 cccttcggtc ctaccgtccg tgattcgtcg ctctgattgg gggcttaacg cctcggagat
     3061 ggctatcgtc tcgatcgatg gatcgacgtt caattgagga ggcgagtacc gatcgagatc
     3121 gattcctgca ccttcgccct cgattgcgcc tgcaatcggc ataacacccg cgctcaccag
     3181 cattaccgcc aggagcaggg ggaatgcccg cattccgatt tttttttttg ttcatcttgt
     3241 tttcctccgt ttttttcccg gaggcaggcg aagcatacct tcaagtacgc ggagcgcgta
     3301 catcctgttg cctccgggca tgcggggctc ttcatccggg gcgtcaaccg gggatggggg
     3361 ggtcccgcgc atctcgggat accggttccg gtacccacgc tgaagaactc aacgttcctc
     3421 tatatacctt ttctgtgaca cgcatctaca cgttggtcct gtgccgtatc ttttccccga
     3481 aaaatcggct tctatgggcg caaaaacggt gccgcgccgg tagggtggga ccgcagttcg
     3541 gtgaacggac gcagtccggt ttcaccgttt cgggccgccc tccggtctat atgacggatc
     3601 tccgggctcg cggtgccttg cccggctgta cctctgccgc tcacgtacag gatgcgagcg
     3661 gcctggcgtt ccggagcgag taactccgga gcataagttc ctccagtgca gcggtgtgat
     3721 cggggagaac ggccggcccg aaaaccccgt agtaaacgat gaaataatcg tcgtgttccg
     3781 gggatagcgg cttttcaacg acgccgaagt aaccggacga tgtttcgctc tcgtatttcg
     3841 tcacggtgat ggggctaacg atcggtgcgt ccggcgatct atccggttct cccgggagga
     3901 tcaacccggc ggaggcgacg ttcccggacg accgaaggta actgcagagg ttcccttccg
     3961 cccgcgcgag atctgcgtca tcatcgaagt accagttgac gaccagatac cggtcaccca
     4021 acacggtgtc gagatacacg gcatgactgc agtaccggga gcaatccgga aacagagatg
     4081 ggcatccttc ttcaatatcg ccgagcagaa cgatacctcc cccgatcgtt ctatttacga
     4141 cgacggtgcc gttttccgcg tactcatacc tgggctgggg gatataccat ttttgcaggg
     4201 cctcgggggg agggtacccg ggaccgatcg tgagaagatc gaggagcaaa agggcgccaa
     4261 aagcaccgac ggcgatcagg aggagaacga gtgcgaccct gccggttctg tcgttttccc
     4321 ggaatttcat caagttcctc cacgctaaag gggggaggtc agggcctcac ccaggttgcc
     4381 atggcaccca accaatcacc atagactatg ttatggatct gagtatccca tgtgtcatgt
     4441 atggacaccc agtcctctgt atagtcgtgg tagccaacac aggcaacgct gtgatcacca
     4501 taaggttcat cttctcctat aggtgtgccg ccgctccaca ggctgagaac aaaaggtctc
     4561 tgtgcatcca cctcgctttt aacctcatca aacgtcatcc aggcatcgtc gcttgaatcc
     4621 attgaagaat atccatgatt cgcgcacact tcatcgatac cgtcatcaat atcccagggc
     4681 cacgtcgagc cttcgggggt agttcccatt gctgtggcaa gttcctgaat caatggatct
     4741 tctcccggga aatttggata tccgtgacta tcccaatagc ccagcaccat cgcggaagcc
     4801 gtaggagagc accccagttg ccataggtat gtcgggacac cgttgatgta tccggatgtt
     4861 ccagtaactg attctgcaga gattgttgga tccggggatt ccatctggtc atctagagcc
     4921 tcccaaagta catttatctc tttttgtttc tcttgctgct gctgaaggaa ctcatctccg
     4981 gttactggga gtcttgtaga ggattcatcg acgaccgaag gcgctgtgag atcgatgatg
     5041 acggaatcct gttttatccc tctcgaatcg gctagcggga acttcgcgta atagaatgtt
     5101 gcacctaaat acagcaactc tgtatcgcct gtgttcagtc cacgcttttc agcgaactta
     5161 cctgcgatct cactcgaatg tgtcatgagt tcggaacatt tgtcgggagt gaacccttta
     5221 gagaactcaa ggatgggata attattccgg gttgctgaaa cgagaatata tccacggtat
     5281 tctccgttaa cgacaacgtc gaaggaatac gctgccggaa ttccctcaag atcgtagtaa
     5341 gtcgtagaga actcaacatt cccgtttcgc caatcggcaa aatccgggat attccctgct
     5401 atttcgttga ccttcagcga agcaattggt gaggctgtgt gtacgctcac cgtgagatca
     5461 cgttccggtg aacattctgt agtctggtgc gattcccccg ttgttgcagc tgcacctacg
     5521 ataggtagca gtaccgaggc tgccagcaga agcatcagca atgctggcaa tacttttttg
     5581 gggcgcataa ttttcacgca ttacacctcc gttttttacc cggaggccga cagagcatac
     5641 cttcaagtac acagagtgcg tacatccctt tgcctccggg cacgcggggt tcttcatccg
     5701 gggcgtcaac cggggatagg gggaccccgt gcatctcggg atactggtac aggtatccac
     5761 gctggagagc tcaacgtttc tctatatacc ttttctgtga cacgcatcta tacgttgggc
     5821 ctgtgccgga ttaccccctc gaaaaaccgg cctccatagg cacgaaaccg gcaccgcgcc
     5881 ggacggcgag atccgtggct ggaggtgcga aacctcctct ctcttcaaag gttgttcccg
     5941 ggtggttgag atgggcccgg cggtaaaacg caatgcataa gaaggttata cgagttatcg
     6001 tcaatagtgt gggcatagtg caggagacgg ggagagaggt agcgatagag aggcttgccg
     6061 ccgtcattga cgaatgccgg aagtgtcccc tctgggagaa cacccatcac gcggttccgg
     6121 gggagggggc taccggggcc ggtctcatgc tcatcggcga agcgccgggg aaacaggaag
     6181 acctcaccgg gaggccgttt gtcggacggg cggggaaact cctcgagagc ctcctttccg
     6241 ggatcggtct aacccgtgac gatgttttca tcgccaatat cgtaaagcac cgtccgcccg
     6301 ggaaccgtga cccccgggat gaggagatcc aggcctgcac tccctacctc gaggagcaga
     6361 tcaggatcat ccgcccgaag gtgatcgtga tgctgggccg gcactcgagc aggtatatcc
     6421 tctccttcct gccggtcgag ttcgaccgga tcacggagat ccgcgggacc gtctacccgg
     6481 gcctcctctt cggccagccg gtccgcctca
//
LOCUS       DAJB01000188            2981 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_149370, whole genome shotgun sequence.
ACCESSION   DAJB01000188 DAJB01000000
VERSION     DAJB01000188.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_149370"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          584 a          886 c          833 g          678 t
ORIGIN      
        1 gaagttcttc ttgatcgtcg ccgccagccc cacggcctcg tcgaggtgct tgccggtcgc
       61 cgggggggcc gcggaggatc gcgttcttcg ggctgccacc ctgaatggcc gggcgggcga
      121 ggagggcgag ttcacggatc tgggcgtcgc ggtggtggag ctctaaattt aaagaaacca
      181 gcggcaaagg gcaccctgat ttatgactcg aggaaatttg gggatatcgg gtaaatcaag
      241 cgaatgcttc taaaatggat caggtgagcc catttggcgt atagtataaa agtgggggtc
      301 cgtgttcacg ccatctgctc gcgggcgatc cccctccccg cgagaatctc ccgcaggtgc
      361 tcccgtccgc tcggctccgg gatctcccgt ccctccctaa actcccggaa aatttagacg
      421 cattgttcca ccaatcccgt tataaacccc tttttcggaa atatctctgt aattctatta
      481 gaatcgaaag gccgagcgga tcacagtgcc taaattttat aggcacatta tgcctcagca
      541 agtgggacat aaaggggaga aatgaagcgg ataatggtga aaggagccac tatcgtgaga
      601 gtagatggcc ccgtgcacct aatcgctgcg ggaggttagg tccctcctcc cagacagcat
      661 cgagccagag gccgatagcc accttcactt cggagagtgc ctcctcttcg gtctcgccga
      721 acgcggagca gccggcaagt tccgggacga cggcgatgaa cccccgtcct cgtcgctgaa
      781 gaagatctcg attgcgtagt tatgcggcat ctcaatcgct ccgaccttgc ggaacctcat
      841 cgggctgttg agtgtacagt tcgacacaat ccaacgccca ttcaaacgcc tcctcaaaat
      901 tcggaacagc cttcatctga tgccggagcg atgcgttcca tgatgctgag aaccggttac
      961 gcctccccgc gagcacggag ttgtccggca cgacctcctt gcattcgcat ttccggtgga
     1021 gaatgctcct cacggcgtcc gggttcacgc ggtcggccag gtgccggata tcgtagatat
     1081 cgcgcggacg tatccgctgg aagagagagc ggatcttctc ggccatgatc tcctccaggc
     1141 agtaacaggt taccccggcg gtgagtctgt ctgagtacgg gtggtagatc cgcctcttct
     1201 caacggggag cagaacgctt tcgttattca tggtcgtgag gtcgacgatg atcttggtcg
     1261 gggtggatgc gttcataggg atgatcctga acaaggccgt cgcccggaac ccggttgtcg
     1321 tctcgatgat gtcgatatcc tcctcgaacc tgatgccgct ctcctctcgc gcacgggata
     1381 ccgcatcctt caccgtctcc cgcagggtct ccttttcgta cggctccagc agggtgaaat
     1441 cgagatcgtc cgagaagcgg tagttctcga tgaagacttt ccggatccct gtcccccctt
     1501 tcagcgccat ggggagggcg ctcagatgag cgaggagcca gttctgtgca tagtcccgct
     1561 cgatagtcga tgggggaacc ccgaaggcac gggcggcctc tttaatctcc acaatcggta
     1621 tcactcggag ttctcctgaa gtgtaatctc ggtgttgatg accagccgcc acctcggatc
     1681 gttctctccc tggtgcggca tcgtaggatc gaggaggaga taccttctgg acgtcgggag
     1741 aggtagatcc agtgggatac ccatctgttc gctcagatac ccgagcctcc ggacaacagc
     1801 gttgttcccg agctgctgcg cgtatcgacc cagggtctcg cgatccagtg atgcgctctc
     1861 cagcgccttt gcgacctcga cgatccctcc cgcgtattcg ggtctgtcga ggcagtcgat
     1921 caccgtcttc tccctgtcgg tgacggtgac cgatatctcc tcgatccatt ccttcttgaa
     1981 cccgaagaac ttctccggct ttacccgcac gatccggtag tccacgccga atacctcgat
     2041 ctggttcttc ttcttccgcg ccgtcgtctg gacaaagacc gttatcggga tctgctctgt
     2101 caggccgtgg tagtggagag cgctccagta tgcaatcgcg gacggccgga cgaggtgcga
     2161 tgcaatcacg aactcgtgga gcgtatactt gcccttttcg ctctctagcg ggattatcag
     2221 atatttcccc ctctcgatcc gctcgacgaa gccccgatct tcccaccggg agagcatcac
     2281 tgaaagcacg ctcttcggga tctctgtctg ccggtgcagt tgttcgaggg tgaatacctg
     2341 tccgttgcgg gcgatggact tcaggatctc aattgtcact tgccgcctct cactttcctc
     2401 atctagtaca ccaaccttat cataattgtt aatttcggtg tactggatgg tgatcgccct
     2461 ccctgttaaa acgcagaaac gcaatcgtac tgtatctctg gatcggaata agcgagatat
     2521 ccggccttcc agttcattcg gggggcatgt gacggagaaa aatctcgtta tgtctcacac
     2581 cagaccacct cgctctccac atcctgccgg atgagaggat cgatccctcc gacggtgagc
     2641 aggtcgacct tccgcccgta gagttcctcc aggaaatcgg cgagcgcgat gaagttccgg
     2701 aacgtgaggt ggtccggcga cagctcgacc agcacatcca catcgctctc cggccggtca
     2761 tcgccccggg cggtcgaccc gaagatgccg atcttcgtca caccgaaccg ctcccgaagt
     2821 agcgggagca cttcttcgag ttttgtgacg aagttgctct tctcctcacg ttgtgcccgg
     2881 actgccatac gggttctgct cgcctccgga tcgtacctgg gggtgaggag atggtcaact
     2941 acccccggtt aaaaccgggg gcttgcagcg gggacccctt g
//
LOCUS       DAJB01000189           12115 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_88573, whole genome shotgun sequence.
ACCESSION   DAJB01000189 DAJB01000000
VERSION     DAJB01000189.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_88573"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    9732..9787
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2151 a         3868 c         3629 g         2409 t
ORIGIN      
        1 gttcagtccg caagtactaa cgtccgatgc cttccgttga actcagacga aatccggaat
       61 acgcaccaaa atccagggag atgatccatc aggatcttcg acctggccag ttcccatggt
      121 aatagcggcc atagcagagg ggaaacacct ggtcccattc cgaacccagc agttaagccc
      181 tctcacgtga tgtgttgtac tgaggtgcga gagccctcgg gaagcacaac tcgctgctat
      241 taccttcatt acattgaaac tctgttcttc tgatagttct cttccacgag cgtatagtct
      301 ctttacgatc atctctcctg taagacgcac gtggttattc ttacctttgt tcacccattt
      361 agcaactgca accacaggcg caggattctc atcccgcact caagtgatcg acccggaaaa
      421 aaggtgtcta ttcagccggt tacgttcgtt gtattatctg tcattgtctc attcgttgcc
      481 agcagggtaa tgtccggcgg gagcatgacc gcatcgatgg cgtggatgac gccgttcgcc
      541 gctagaatat cgacccggac cacctctgca ccgttcaccc tcaccccgtc gccggcggcg
      601 tcgacagtca cgccgttgcc ctgtatggtt gcaatgatat catttgaggc tatgccggaa
      661 gccatgtagt ttcccggggc catatggtag agcaggacct ctgcaaggtt gcccttcggg
      721 tcgctgaaga gctcttccat cacggcattt gagatccggc tgaaagcctc atcggtgggc
      781 gcgaagactg tgtaaggtcc cggaccggcg agcatccctt cgagcccggc cgcctcgatc
      841 gcctgggtga acgtcgagaa gttgccgtcc cggtccagca cctcattgat tgccaggctc
      901 tcgacgaacg gtgtctcgac aggcgttccg ttcgtctggt tccgggcatc acctcctgat
      961 aggcccatgc aaccgcagat gagaactgcc gccccgatgg ccagagcaat gatgattgtc
     1021 gcgtaattcg ttcgcatggt ccctcctgaa gactgtatgc tccaaccctc ctctctcctc
     1081 acccctacat aaagtattgg tctcgatcgg atccggagca cccttatttc cagagtttca
     1141 gggtagagac gacatgacgg acccgatcgt agaccctgac ggtatggttg ccggtatcgg
     1201 cgatgtacca gagccccctg aacttgacca gaccgcccgg ctcattcagt cttgcgtccc
     1261 ctgataaccc gtcccggtag ccgcgctcgc cgtccccgac cctggtgagg acccagccgg
     1321 tctccgggtc gaactgtttg atcttatggt tgtaggtgtc ggcgatgtag agcaactcct
     1381 ctccgtaggc gatgcccccc gggtggtgga tacgcgccat ccctgcgatg gtgtcgaggt
     1441 cgccgaagtc ttcaagggag tgcccgatgc gtgtctcgac cataccccgc tttatgcgac
     1501 ggatcgccga agctccactg tcggccacgt agagcgcctc gccgtcggtg acgatcccgc
     1561 cgggcccggc gaaggcagcc tcttcgagcg ggccgtcgac caggccctcc cggcccgaac
     1621 cggcataagg ctctatctca tgggtggcga ggtccatccg ccagacctga ttcgctccgg
     1681 ccatcacgac atagaggtag tcgcccagca gcgccagatc ccgcggcgcg ttcagcgcga
     1741 ccccggtgcc cggtccgcct tcgccgggcg agggtgcgag aagccccgtc ccggcgatcg
     1801 tctcaacccg ccggtccggc caggagacct gccggaccgt gtggttcccg gtatctgcaa
     1861 catagaggat gcctgcctct tcgtcgaacg tgaggccctc cggcaggtag aacgccgcct
     1921 catcaaacga cccgtcggta tttccggcag ccccggagcc gatcgtctcc aggatcctgc
     1981 cgtcggcgtc ggcgacgacg atccggttgt gaccggaatc gcttataaat agccgcattc
     2041 ccgcgttgtc tgccgcaacc ttgccgggac gatagagggc tccttcccgc accgtctccg
     2101 gcgcggtgcc gtgcagccct ccactctcca gcagaccgcg ctgcttgaac tcagctgcga
     2161 gccggtcgat cttcgggttg agccgtccgt agagcccctc cccggtggtc ttcccgacca
     2221 cgttcccctc cgggtcgatg aggacgaagg tcggccagtc ccggaccccg aaggccggcc
     2281 agagcaggtg gtcgtggtcg atgacgacag ggtgctccat tccggtacgg cggaccgcct
     2341 cccggaagtt cccggtgacc gccccggatt cgaacccggg tgaatggacc tcgatggtca
     2401 ccaggtccgg gtacttctcc tcgagttgcc ggatctccgg caccagccgc atgcagtttg
     2461 aacaggcgaa cgtctgaaac gagagcagca ctatcctgcc ggagaggtcg cggacggaga
     2521 gggggcggtc gatgttgagc cattcgagac ctgcggggaa atccggcgca gtcatctgtt
     2581 tcatgcggca cactctggcg tttcagggtt atgaatgttg gcgaggaccg ggggtcgggt
     2641 gacccctcgc cggtcccgtc acgccggcgg cccggcgtac tccacccgcg gcgcggcgag
     2701 catctcttcg cagaaggcaa tcgccccggg aaccctgctc ttctggtaga ccacgggttt
     2761 gatccgcggt ctccccggaa ggctgccgat cagcgtgaat atctgtcgcc agggcaccct
     2821 gtcggtcacc gacggcgggg cgaggtcgct gtgaatgtga aaggcccgca gggcatcgag
     2881 cgggacctca ccgagcgatc ccgggaagtc ttctttcgtg gcagcatgga ggtgccaggg
     2941 gtcgagcgct atcccggcca gtccggctat ctcgggatgg atctttttga gcattgccca
     3001 gaacgcctgc aactggctcc ctgtggagat gctctggtcc ttatggtgct caagcagcac
     3061 caggggttcc accccgaagg cgttccagtg ggcggctatc agggtatgca tcgccgtgac
     3121 gatatcggcg tgggcgttcc tccggccgcc ggggtgtatc tcgatgatct ccggagggat
     3181 gcccagatag tcttcgatct cgatgagcat atccccaaaa gctaccaccc agtccgggtc
     3241 gcgccaccgc agctccgccg tcacctgccg gccgtgctgg tctcgccggg ggatagccgg
     3301 ctcggtgtgc aggagatact tcagagatcc gggaaggact gtatccttct cgtaaaggtg
     3361 ctcgatcgac gaccggctga gcatggaccc gatctcctct ccggtccgct ccacctctgt
     3421 cttattcttg atgaagtcgg cggggacctc gatatacatg cacccgaacg gcagagcaag
     3481 gtgccagcgt tcgaggagcc cgccccgccg cgcgacttcc gtgacgtttg gatagatgta
     3541 atccgcacct ggcatggcaa tcagtctacg ccatggccgg agtggtacaa aaagattggg
     3601 gcgggggtac tgatgcatgc ggggcttttc gtgctgcgac gaagagccgg cagtcgccgg
     3661 aaaacggatc gccctcgggc tacctgcggt tatcgagggc ctgcggacag acgaacagga
     3721 tcgatacggg ggtactgatg taggtgcagt aggtgcactc ggggtcgtaa tacctgcacg
     3781 aggtgcatcc ccactgcgcc ggtttccctc ggtctcgctg tctcactgct gcgggatctc
     3841 cgtcccggtc cgacgtttca ggtgggcgga tgcctgtctc cctctccagc aggccggcgt
     3901 tctccggtag actcatggtg gtgcaaggat ctctcggata ggtagacatt aattgcggcc
     3961 atgaagattc atcggactga tgaatcccgg tcctccggaa gcaccgtccc ctctctgcca
     4021 gattctggca gggagactcc cgaactgcct gaaaaaccgg ttattccgga ggcgtccgca
     4081 gtggccgcgc ggttgcttta tagtgctgaa acacccgttc gccccaggcg accgccgccg
     4141 catcggtgcc gatgagatct gcagcggcgt cgtacatatc cgtatcgcgt ttatacaggc
     4201 ctaacgacag gtattcgtcg gtcaccgtca ttcccaggcg aatcggggag ggagatacgt
     4261 atattcctga gcgggtatac tccttcaggg actcgataat ggtccggtac ggctcttcgg
     4321 ccagcctcgc ggcgagttcc ggggagacga cgagttcgac cggcttgctg tccataacta
     4381 cctttttgat cacgtctaca tggaccgggc tcatgatcga ggatacgccg tgaatccatg
     4441 atgctctccc gagtatgcct gcaaaagctg tgtgtacggc atagatgtcc tcttcgacgt
     4501 cccggaccac ttctgcaccg tagaggtcct gcaggtcgct caggaactcc ggaggtatgc
     4561 tttcgatctc gtgttcgatc cagaagtccc ggtgacgctg gagcaccccc atcaccatga
     4621 ccagccgctc gatctccggc tcgacgattt ctccaatcgg ggtgagcgcg tagtcgcccc
     4681 gtattgattc gatgtaatgc agcgcctcaa ggtgccggat cttcgggatg agcgcctgcg
     4741 acgaacttcc cgtgacatca cgaagggctg aaagaggttt gctcccgctt ccaagcgcaa
     4801 tcagtatctg cgtcaacaat ctcgaccgga agatcgcctg gaggtcgtca tggaactcgt
     4861 tatacatttc cagaggggat gttcctttca tcttaaatta agaggtgccg gtcgacgcat
     4921 atcacccttt ccattcctgc gcgccccctc cacccgacgc tcttttcccc ctgtcgaccg
     4981 atcggtcgcg tgcccggggc ggggttcatc tgcccccgca ggtgctcttt ttgcccgacc
     5041 tcagcacact tatagccggg cgccagatac ctgtagaggc agcatgtcgt ccatacctgg
     5101 atacctgatc accatcgcac tctgtacgtg catcatcgcc tgcttcatca ccgcaggctg
     5161 ctctgctccc atcgctcagc cggagatcgc ctccggcctc tccggtgacc tcgtcgtcca
     5221 cttcatcgac gtcgggcagg gggactcgat cctgatcgag ttccgcgata agaccatgct
     5281 catcgacgcc ggggagcgcg ggatggggga gcgcgtcatc gcgtacctcg atgagcgaaa
     5341 cgtcgaggaa ctcgacgtgg ttgtggcaac tcacgcgcac tccgatcaca tcggcggcct
     5401 cggcagcgtc gtctccgcct acccggtcgg ccggttcgtc gatgccgcgc agccgcactc
     5461 taccgcgacc tacgaggacc tgcttgtcct ggtcgaggaa caggggatac cgtatacggc
     5521 agcggagcgc gggcagacga tcgccctcga tcccgacctc gagatactgg tcttaaaccc
     5581 gactccccag ccgctcggtg acacaaacga ggactccgtc gtcctgaagg tgacgtacgg
     5641 ggagatctcc tacctcttta tgggcgatgc cgggaatgcg gcggaggaga gtatgatgga
     5701 ggccgggctc gacctcgacg ccgacgtcct gaaggtcggc caccacgcga gccgatacgc
     5761 atcctcggcc gaattcctgg ccgcggtcag ccctgcgatc agcgtcattc cggtcggtga
     5821 ggggaacgat tacggtcatc cccacgagga ggcggtcgaa cgcatcgagg cgaccggctc
     5881 gcgtatctac cggaccgacc ttgatggaac cgtcatcgtc gccaccgacg gccgggcgct
     5941 taccgttgcg gccggcggag caccgtccgc caccgtgacg gcccgggcca cgacatcgac
     6001 tccggcagcg acctcctccg gcgtatacat caccgacctt gacatccagg acgagtggat
     6061 caccgtcacg aatgcagaga cggcagcggt caatctcacc ggctggacga tcaccgacga
     6121 ggggacgcgg aatacctaca ccttcccgct cttcaccctc agcccgggtg cagacgtcac
     6181 cgtccactcc ggtccgggca acgataccgt gtccgacctc tactggggcc gggagacccc
     6241 ggtctggaac aacgacggcg accttgccac gctcgccgat gcgaacggca cggtcgtcag
     6301 caccctggag cggtgatgac gcaggacgaa tcaaccctcc gggtcagcct cgaccgggtc
     6361 gaggagggtc tcgccgtcct cctcctccgc gaggacgagt cggtgcgctt cacgctcccg
     6421 cgctccctcc tccccggcga cgcccgggag ggcgatatcc tcgagatcgc gatccgccgg
     6481 gacgtcgcgg cgacggcgga ggcacggcgg cgggtggcgg agaggatcga gcggctgcgg
     6541 aggaagggcg gagactgact tccccgccgt cgccacccca ctcttttata ctcctctgct
     6601 cctatgggag cacgatgctg gatcggtgtg acgtgctcgt cgtcggtgcg ggccccgccg
     6661 gcagcactgc cgcacgctac gcagcagagg cggggctcga tgtcctcctg atcgataagc
     6721 gaaaggagat cggcgttccc gtctgctgcg gggagttcaa cgcatctctg gcggtgctcg
     6781 ccgaagcgct ccccaatgcc cccgggctcg atgaactctt ccccatcgac cccgggctgg
     6841 tccggcggaa gatccggggc gccgtcgccg tttcccccgg cggccgggag tatgagttcg
     6901 accttcccgg ttacaccgtt gaccgggatg cgttcgaccg gcacctcgcg gatgcggccg
     6961 tccgtgaggg cgccgtgctc tcaccggata caatgttcct tgagagggat ggccgccggg
     7021 tcaccaccag ccggggcgat atcgaggcca atgtcgtggt cgccgcggac ggcccgctct
     7081 cccgggtctg ccgctcggcc gggatggagc gcagcgccgt gctcgccccc gcgatcacct
     7141 gccgggtaga tggggagttc ggcgatcgcc tgcggttctt cttcgggagc agcgttgcgc
     7201 ccggggggta tgcctgggtc ttcccgaaga gggggtgcgc caacgtcggg cttggcgtgc
     7261 agaggccggc aacgcccgtc cccatccttc tccgggcgtt cctctcgcac aacggctttg
     7321 ccgcccggga tatccaggcc ggtttcgtcc ccgtctccgg ccccatcggc gagaccgtgt
     7381 gcgagaacgt cctcgccnnt cgcctcgaac gggggcggta ttgcgcccgc aatgctctgc
     7441 gggaggcttg ccggacttac cacggcgaag caccttctgc acggcgagcc tctcggggcc
     7501 tacgagcagg cgtggcgctc agcaataggc cgggaacttc ttgccgccgc aagaaccaag
     7561 aaggcggccg accggatcct cggctccgac ttcctgctcg aacaggctat gggtatactc
     7621 gggggccgcg gtatcaggaa catcatcgtt tttggcggcc tcaggggacg gtgacgaata
     7681 cctgactgca caccttcatg gggatgagaa ccggaatcct tattgtcccc gggcgagtcc
     7741 gtgccacacc accgggaggt gtgtgaattg gtcgatattg tcaaggtcga tggcagaaga
     7801 gaaccgttcg tgcgggagaa ggtgactgtg agtgcgctca aatccggagc cccgccggag
     7861 gaggccaggg ctatagggga agccgtcgaa cgggtcgcct acgatgggat gccctcgggg
     7921 gagatacggc ggcgggtgct tgagcagctc cgcgaccgga accctgagtg ggaagaaaac
     7981 tggctcatgt acgaccgtgc tgtgaagaaa cgcggggcga cggcggccgt ggggctgcct
     8041 gcccgctgat ctcaccgctt tttttcgagc cggccgggca gcggcgcatt gcaccccgcc
     8101 gcgaggccgg catacccgcc ccgtcatgac gaaactatat ctactgatcc gcgcccatac
     8161 tagtcttata ttgtcatatg ctggccggaa atccaataat tgaaatgggc gccagcaggc
     8221 cgttttcagt ttggccacct atccggggca cattcgacaa tatacatcca cagaaaggca
     8281 cgacgtcccg cccgcactcg aaacggttcc cgatttcacc gggagctcgc ggcatgggca
     8341 cgccagcagg cgatatgtat gccgacaagt gactcagaag aagacacacc ctctaataat
     8401 aaccagaaag atacagaagg aacaaagata ctcctcgccg atccgaagac cgcgatcctc
     8461 aggctatccc tgccgatgat gatcgcgatg accctcatga ccctctacag cgtcgtcgac
     8521 gcgttctggg tctcgggtct cggtgcagac gctcttgccg ctgtcgggtt ctcattcccg
     8581 ctcttcatca taaccatcgg attagccagc ggcctcggca ccggcggtga agcggcgctc
     8641 tcacggatga tcggcgcccg caacagggat ggagcgagca gcgtcgccat gcacacgatc
     8701 ctcctgatga ccgtccttgc cgtcgccgtc accatcccgc tctccctctt cgccggggac
     8761 ctcttcgtcc tcatgggggc gggcaacgcc gccggccttg ccaccgagta cgcccggatc
     8821 ctctttttgg ggacgttcgc cctgctcttc ggcgaggtcg cctacgcgct catgcacggg
     8881 gaaggagacg caaagcggac catgtacgcg atggcggcag gagcggtggc aaacatcatc
     8941 ctcgacccga tcctgatcta cacgcttcat atggggatcg ccggcgcagc ctgggcgacc
     9001 atcctcgccg aggtgatcgc cgccatcccg atggtctact ggctcttcgt gaagagagac
     9061 acatacgtct ccctccgcct ccgggaattc tcccccgacc ccgcggtcgt gcgtgacatc
     9121 ctgcaggtcg gcctccctgc cgcggccgag cagctgggac ttgcactgat ggccctgatc
     9181 ttgaacggcg tcatcgtcct gatatcgagc accgacggcg tcgcgatcta ctcggtcggg
     9241 tggcgggtcg tgagcatcgg cttaacacca atcctcgcca tatcctcggc ggtggtcgcg
     9301 gtcaccggcg ccacctacgg cgcccgtgcg tatgccaaga tggagtctgc cctcctctat
     9361 gccgtcaggc tcgggctcgg catcggtctc gggcttgccg cagcgacgtt catcttcgcc
     9421 ccccagatcg ctgcaatctt cacggcacca ggcgacgcat cggggatcgc cccggagttg
     9481 acgacgtacc tccaggttac gagcctcgtc taccctatgg tcgggttcgg actcttctcg
     9541 tcgtcgttct tccagggaac aggtcttggc gcacgatcgc tcacgatcac gatcctgcag
     9601 aacgtcgttc tctcgaccct ctttgtctgg gtgtttgcac tcgaactcgg gttcggcctc
     9661 cccggcgtct ggtggggagt tgccgcaggg aacgcaatcg gcgccgtgct cgggtatatc
     9721 tgggcgcgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnntcc ggcctcccct ctatccggtc ctcaacccag atccggccgc cgtagcgctc
     9841 gatcagcatc ccgacgatgt acaggccgag cccttccccg cacccctggc tcttgccctg
     9901 cctgaaccgc tggaaaattg cctctttcat cccgtcgggg atgccggggc cgttatcctc
     9961 caccaccacc accaccgtgt ggtcctcttc gggatagtcc tcgacccgga tcgttatcgc
    10021 ggcgtccggt ccgccgaact taacggcgtt accgatgagg tttgtgaaga tctcggggag
    10081 caggtcgtcg gcccagacct cggtcggtgt atcctcaaac gagacacccg cttccggagt
    10141 acggtcgagc ccggtcctga tcacatggct gaggtggacc ggttcaaggt cgggcggctc
    10201 ctggtgaatg cggcggatgg tcgagatgtt ctggaggata ccggtgctct tccggatact
    10261 ccccttgagt ttcctcgcgt actgcgcggc gtctccctcc agcatatcaa gcaggaggtc
    10321 gcagtagagg ttcgcgacgt tttccgcgtt cttgatgtcg tgggtcatga catcgaggta
    10381 gaggttcgtc tcgcggtggg ccgcctccag ccgtttatgg agcatgctcc gcaggacccc
    10441 cgaaccgatc tcactcccga tcgcttcgag aaggcggcgc tcctcgtcga aaagttcctc
    10501 cctcttcctg ctcccgagaa agagcgcccc gaccacgact gactctgcaa gaatcggtat
    10561 gcaggcgagg gcacagaccc cgagagatga gaggatatct tcctcgatcg taccgccctc
    10621 gcctcgcagt tcaaggtagc gcggttgtcc ggcaacaaag acaaaattcc agggccagtg
    10681 atggaccttg atagccctgt tgcgggcgag gtaggtctcc gggacctcgt ggtggtaacg
    10741 cgtgagtgcc agcgttcgtt ccgggtttaa gaggtaggtc agtcccagat cgaagtcgag
    10801 caggtccagt gtctttgaga gcgatgcctc gagcagttca tcgagcgaga gggatgacgc
    10861 agatactccc atgatctggt tgaggatcag gagctgtctg ttcgaggaga gcagcccttc
    10921 gttcgtctct tttcgttcgg taacgtcccg tatcctgagc acccagatcc ctgcgaacgg
    10981 ctcctgctct atcttccggc tggagtagac gaaccgtcgt tcttcgcccc gggagtcctg
    11041 caccgagaaa tcgaggccgg gtatctcgtc ttcgtcacgc atcgtggcga ggagtctgtg
    11101 ggcatcttct ctcccccgca ggagcggcac caggtgcgta tcgagaacct ggcaggcatc
    11161 gcatcctaaa atctcctcct cgcatgccga gaggatatcc atggcataag cgtttgccca
    11221 ggtcacctgc agatcccggt cgagcacgag ggtacccacg ccgggctcgt cgaggatctg
    11281 aaacgtctct cgtggttgga tagatttcgc attcgttcta ctcatgtgag tatcgtgccc
    11341 ccttgaggat attatcgggt aaaaattata taaataattc atagccggag tgataagaga
    11401 tttgcggtga tggcccggga aaattcggat cgccgggcag caacggctgg ccgcgcaaag
    11461 atggttattt ttgaagcggc aggcggcctt ttgcctcaaa aacctcggcc ggagccccgc
    11521 accccggctc accccgttgc aggggttatc ctggaaccac cattaaagaa gccctatccg
    11581 gctgagtgcc ggagtgagcg acgtcatatg gccctggatg tagaccgtct cacgggggtc
    11641 ggcgcccatc gcgagcgcca ggagttcggt gatatgaagc accggcaccc ccatatggtc
    11701 gtacaccagc tcgcaccccg ggcagtcgac gaccatcatg tccaggcgat gctgtatgaa
    11761 atcctggcgg acgagccggt tcaatggggg gagttttaga ggatggcttt tctggtgccg
    11821 gtcgaagagg ttcttgaccc cgccgcaaca gtagttctcc atcccgctcc tgacgacctt
    11881 caccccggtg acctctgtta ggtcctgcaa ccccggcggc acggcgaggg tgccgttcat
    11941 cttccggtag tggcagctga catgcacccc gacgcgcagc cccgagagcc gtgtctcgac
    12001 gagccgtctc aattcgtcga ggtagagggg gtagaggtcg aggatgtttg ccacacaaaa
    12061 catccccggg tcgctcactt cccgccctat cctgcccagc gcttcgttca ccacc
//
LOCUS       DAJB01000190           36757 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_55675, whole genome shotgun sequence.
ACCESSION   DAJB01000190 DAJB01000000
VERSION     DAJB01000190.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 36757)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36757)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36757
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_55675"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    6941..6961
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7114 a        11599 c        11412 g         6611 t
ORIGIN      
        1 agccggatac cgtgttcgcc ggtagctgcc gggagaatca aaaaaagagg ggcgatatcc
       61 caatttttcc ctggaacggg agcccattcc ggccccacta cccgctcatc cccatcctgt
      121 cgtcgagcca gtcgaacctg gcggcaaacg attgcgcgaa cgccccgagc tggcagtgcg
      181 acccggcacc gtagtcgtcg gagaagacca tacgttcccg ctcgcaggtg aggtggccgt
      241 agagtgccct tgcctgcacc ccgtccgggt cgaagtgatc ggccgcaccg gcacagacca
      301 gcgtggggca ccgtatctct tcggcaattc cgacgagtga aaaaagattg aaaggatatg
      361 cagatcatct ggacgatcgc ggtgtccacc agtgtctacg cctttgcctt gagtttctcg
      421 atctcctctt caacctctcc cgcacccttc tggtagaatt ccttctcccg ggggtccagt
      481 tcgatgagga ggcgatagaa ctcaccgacg tgtaccttct cctcgtctgc gatatcccgc
      541 agaacctcgc gggccagttc attgtcggtt gcgtcggcca tctggttgta gagctggatg
      601 gcttcgaact cggcggcgat cgtgtagcgc acggcgcgga tgagttcgtt gtggctcatc
      661 atccggtcga tgttgtttcc ggcaaacggt tgtgcaaatt caggcatgta gttccctcac
      721 aagaatcgtt ccggccgctg cagcggggcc ggcgatgact ggtttgctta gaaaagatat
      781 atgccggtcg gttctggcat gcggcgtgag gagagagatt ccgggcccgg tcggcagaaa
      841 atcacggaca cggagcccgg tgcggccgtt cgcgtatgca tccggttcag ctcccgcggg
      901 ccggaggaga gaaagacacc cccgccaggc tgtgggcagg atcgatctgt ttccggaata
      961 ctcccgttaa aatcaataat atccaccgat cagcacttta ggggcccgga tattttccat
     1021 atgtctaaat actatcctat aaaagttaca cctgaaccca agtattctgg tgattgaaat
     1081 ggctaacagc cgggtgaagg gagttcgccc gataacactt ctttttgcgc tgacagtgct
     1141 tatctgcgcg atgctgatag caccggcagc tgcagcggcc ctgccggaca gggtcaatgt
     1201 gaacgcatcg tacaacggag aaacgcccga ctacgggacc cttaacttct cgaccatcca
     1261 ggaggccgtc gacaacgtca cgaccggtgg cgaggtatgg gtctacaacg ggacatacaa
     1321 tgaatcgatc gtgatcgcga gaagcatgac cgtcacgacc gtctcgcact tggtatgccc
     1381 gggcctcaac ggcacgacta tcgacgccat gtatgagccg gtaggcatcg agatcaacgc
     1441 ggacaatgtc tccgtttccg ggtttgaggt cgtgaacgcc tcagcagtcg ggatcaatgc
     1501 ccacagcgtc agcgcggtca ccatcgagta caactcggtc gtggttcgca gcgacaccta
     1561 cgagctcacc tgcggtatcc tcatcgaagg gggcgaggga gttgccatcg gcaacaacga
     1621 ggtgcgtgtc atcgggagcg gcggacaggg cggcatcatg gtcgagggcg tcactgaggc
     1681 ctacgtccac gacaacgacg tcgtggccgt ctcgcggcac ctggatcagg aggctgccgg
     1741 gattgaggcc aatccggcca ttaccgccct cgttccggag gatgacggac ttctctccat
     1801 cgcagtggag gacagcccgg tgctggagcc caatggggtt ctgatcgtag agagcacctt
     1861 tgtcctcgtc gagagcaacc gggttgtgtc cgtgggcgcc tgcatcccgg acgagcagga
     1921 cccgcactac gcggccgtgg acgcctgggg tatccggtcc agcgtctccg agaacctgaa
     1981 ggtcctggag aacaacgtca cggtcgtcgg tgttgcagcc aacgccacac ggacggtcgg
     2041 catcctggga accggggacc tcgcgctcat ccagaacaat acagtcgaga cctataccga
     2101 gtcccagctc gtccagccgg taggcatcgt gctcgaccgg gcttcgaagg gacaggttct
     2161 tgataacgag gtcgagatgg aggtcgagtg tgtcggcacg tccggggaag agctgatgga
     2221 gtctgccggc atcatcgccg tcgagtccta taaggctcag gttaccggaa acgacatcca
     2281 ctacatgctc ctggccgccg gaccggagaa cctggctctg gcagagatcg agggtatcga
     2341 tatcgtcgag tgcgacgagc cccgggtaag cgagaacatc gtcatcgtga tgtccgagat
     2401 cgccgtgtta ccgactggcg atgaacccat ggagggtgaa gagtccttcg tcgcggcgtt
     2461 cagcagagtt acgggcatcg acgtcgagat gagcgacgag cccgagatca tcgagaacgt
     2521 cgtgcacgcc ttcggcaccg ccatagccta tgacggaacg gccgcggacg aacccctctc
     2581 ggtcgtcgcg agcgatctcg tcctgacagg catctgggtc tggggcggct atgacgaact
     2641 ggtctccagc cccgtgatcg atgacaacgt tgtcgtggtc cgggcgcagg acgcaggaat
     2701 tgccggcatg aacccctact ttgaggatgt agaggccgcc atcgcggaca acagcgatct
     2761 tttgacccgc tacgtcagcg tgatgcaggg cggagaggaa ggatcttccg ccgcgacatg
     2821 cctctcgaca tacctcaccg acgctgagga acaatccgtc gtcgcgaaca gcgtccttga
     2881 gtacgaaccg agcatcgtgg aggccaccct cgtcgaagcc cgtgtcgcaa cggccggcat
     2941 cgtcctcgaa gacgtcgtga acccgacggt ctgccacaac tacgtgccgg tgcgccagag
     3001 aatcatcgtc tacgcagtcg acggatcgca gcccggaaat gtcgggctct tttcgaacac
     3061 cctgccgata gggcttatcg agcaggctgt cacgggagaa gaaatgcagc aggccgtcct
     3121 gctcagcctc atcgacgaga acgacagtga gtggcagaac ctttccccgg cggacagaac
     3181 ggcaatcacc gatgccgtcc tctccggaga cggggacgtt cttgcggttt acgcagagaa
     3241 cgagaccctc tttgccgccg actccgagat ggcagacacg ctcctcgagt atcaggcact
     3301 cattgaggac gagggcacgt tcgagttcct gaacgtgacc cggcttgcgg ccacggtccc
     3361 ctactcgatg agttacggtc tcctcttcta tgacgccgaa ggtgacctgg agatctacgg
     3421 aaacgagttc ctggtcgaga ccaacgccat cagcgttgca cttgccgagg gcgacaccga
     3481 ggtgcccgat gccgcggcag ggtccgccgg cctcgtgacg gccttcggta tctccggaaa
     3541 cggcgatagc atcaacatcg aagagaatac catcaccgtg gacacccagg gcacgttcct
     3601 gaacctggcc cgcggaggcg agaacgagac cgccgacgtg gcggcaggat ctgcgcacct
     3661 cctccttgcg gtgggagtca gtgcggacgc ggatgatgaa ctcatcctca acaacaacat
     3721 caccgtcatg cagaagacta tggccgaggc cgaggccatc aaccgggtaa aggacaagac
     3781 ggccctcagc gttgccgcta cggcaggcgt gggcgtcggg atcgtcctcg atgcggatga
     3841 cgtctttgac cccgacacct acgagcccgc tccccccatg ccggaagagt ggttcgcaga
     3901 tgtcatccag ggtaacacca tctccgtcac ggatgagatc gaggttgcct cgatcgccgg
     3961 gatgctgctc cccgcacccc tcgacgggtc atcgaacgcg ctctccggcg cggtaggagc
     4021 cgcagcgagc ttcggcatcg ttgcacccga ggccgaagtg ctggataacg tcgtcaacgc
     4081 aaccgcgagc cagagcagca ttgcagcagc gttcgtcgaa gaagtcatgc ccgtagcgct
     4141 ggcagcggag atcgacctcc ccggcaccgg ggccgcgaac ctgggagtcg cagtctccgg
     4201 cggcatcctg accctgcgct cgtacatcgc gaacaacatc gtcttcacgg aggcgaactc
     4261 tgaaacagtc gttgaggcag cgacggagga actcctcgag gacgccggcg ttctcggagg
     4321 caccatggcg gtcgacgtcg gcatcgtgag cctgagcccc tccttcatcg acgggaacag
     4381 cgtggacgga gcggcatccg cgagcgccat aggcatggtc gggggcgaca gggtggacgg
     4441 agaggtcctt gtcctgggta tgaacctcgg gatcctctcg cggggcagcg acgcgaggtt
     4501 caacaacatc tataacggct acatagggtt cccgtactac tacgaagagg aggagtacga
     4561 accctacgcc ttctataact ggtggggcga cgcgagcggc ccgtccgggt tcggtcccgg
     4621 cacgggaagc ccggtcaccg gcaccatgaa ctatgaaccc tggctgacga agccggcaga
     4681 cgtcgtcctt gagaccggca aatccaattt cggccttgag atcggatcgc ccaacatcgg
     4741 cggcgacggc taccgctacg gcctcgagcc gggctggaac accctctcgt tcccgctggc
     4801 gcttgagaac aacacctggc aggcggtcac ccacgccggc gacggcctcg actacgcggt
     4861 cgcctgcacc tgggattcaa ccaaccagcg gtgggtccag atgactgcga cgagcaagat
     4921 caacccgctc gattcggtgt acatcctgat gaacgactac gaccgcctcc cggtcgcgat
     4981 cagccccgat cccacgaacc cgccggtgaa gatgctccag gccgggtgga accttgtcgg
     5041 cccggcgtat gacctggtcc cgcttaacga gccggtcgac gacatcaccc tctggtgggg
     5101 cgagccctat gggacctcgc tcgtcaaggc gctggcctcc gtcgagcaga cgcccgcagg
     5161 cctcaccggc tacacggtcg tcgtctcccc gtccatcaac cccgaatcct gggtctacac
     5221 tccgggtgac gggacagaac ccgatatgga cgccacctgc ggctactggg tctacatgaa
     5281 gaaccccgtc gaacttgtcg ggttctcctc cacccctctc gacatgccct ggtgggcctg
     5341 ggacctgtga gacggagggc ccaccaaccc tttttgagga acaaccatga agcgaacacc
     5401 agcaccacga accataccca tcctcacgct cctcatcgca gtcagcctcc tcgccgtccc
     5461 cgcactcgcg gacggcggtg ccccgtcgac gatcccgcac tacttctacg ggcagatcta
     5521 taacgtagac ggctcgaatg ccccggcagg ctcgatcatc gtcgcatcgg tcgcggggaa
     5581 ccccttcgga atgaccaccg tgaccacagc cgggcagtac ggggttaatc gcacggacgg
     5641 ggacgccgtt aaatttgctg tctgggatcc cgccatgtct ccgggcgaca ccatcgtctt
     5701 ctacatcgac ggcgtccggg caacggagtc ggcgatctac gagagcggcg gcttcacgaa
     5761 cctgacgctc accgcgagcg cggccctccc gaagaaacag cccggcgaga gcacgacccg
     5821 gccggtcaat gccacggccg gccagcccgt cacggtcgac ggcggcggcg cctcggtgaa
     5881 cctgacgaca aagggcggct gccagggcga gacgctcgtc tttacgctct tcacgcagcc
     5941 ccccgacgac caggcgatcc cgccgggcaa cgataacatc ggccggtttg ccgagatcac
     6001 gagcagcatc acaaacgaca acatccagaa ggtgatcgtg acgctccact acaccgacag
     6061 cgatgtcgcc gggatcgacg aaaactccat ccgggtctac tggtggagca cggcgaacag
     6121 cacctgggtg cagctccccg gcggcgtcga caccgccgcg aacatcgcct ggggcgagac
     6181 ggaccacttc tcgaccttcg ccctcctcgg cgtcacgccg cagccggcgc cgacacagcg
     6241 gggcggtggc ggaggttcga gcggtggcgt cttcatcacg cccacctcga acgtcacccc
     6301 gacgatcacc ggagaggaga cccccacccc ggtgaatgca accgagacct ccgcggccac
     6361 cccggccgtg acagggggac cgacgaccgc gacaggcgca caggccacgc agggtgaacc
     6421 gggtgcggaa gcacccgggg accttccgat gaccgcgatc gccatcgtcg ccggtatcgt
     6481 cgtgattgcc gcggcagggt tcctgctgct ccggcaacgc tgatacccca accctttttt
     6541 ccagaccgcg atcccgtcgg gtgatcgttc gttaccgctc tccgtcaccc ggaaccttga
     6601 tgggcaggag ggagagacct tgcaggagag aggcccggag accgccatga tccttccacc
     6661 tgcattttac gagcgagata ccgtgaccgt cgcaaaggac ctgctggggt gcctgcttgt
     6721 gcaccgggag gatgcaggga cgacgatggg gtggatcgtc gaggtcgagg cttacctccg
     6781 ggacgacccg gcagcccact cgtaccgggg agagacgaag aggaatagcg ttatgttcgg
     6841 tccggcgggc catgcctaca tataccggat ctatggcctg cacacctgcg tcaacgtcgt
     6901 gaccggaccg gagggtgcag gggaggcggt cctgatccgg nnnnnnnnnn nnnnnnnnnn
     6961 nggtcgcccg cgagcctgcc cggccgccgt ccggaaggct cggaggggga gatcgtggag
     7021 acgacccgga tcgggatcac gaaggcggcg gatctccccc tccggtatta cctgaagggg
     7081 aatcgatatg tctcgcggcg gtgagtcgcg gggctattcc tgatggcccg gaggggtccg
     7141 ccgccggacc gtcctctcgc cctccgctgc aacctgcccg gttatcaata tggtctgttc
     7201 ttttaccggg atctcctttc ccgagcgttt cagggcctga tccacgacat acctcacacc
     7261 gctgcagcag ggcacctcca tgtgcaggac cgtgatcgag cggattgtat gctgcgagaa
     7321 tatctccgcg agtttcgtga cgtacccctc aacatccgtg tccagtttcg ggcagaagat
     7381 gatcgcgatc ttgtcccgca gaaaatcccg gtgaaagtcc gcgtatgcaa agggaacgca
     7441 gtccccggag atgaggagat ccgcgtcgtc gaagtacgag gctgcggcat tcaggagctt
     7501 caactggacc ggccactgcc tcaactctga ttccgtccgt gcggcacgct cactctcccc
     7561 cgcatccctt ctcggaaggc ttcttgcggc ggagcccgga caccccgtcg gaacactctc
     7621 tgtagcacct gcatggcctc ccgcggtgtc gtgccgggga accggaatcg cgttcgcggt
     7681 caggtactcg atcgcctggc ggtacaggtc cttctccccg tggccgataa ggtgctcaag
     7741 gtgcgcctta atgaccgctt ctccctgggg ggcgattgtt gccatcacgg ccttctcatc
     7801 gtatgggccg gcctcccgct cgatgacgca gatcgccccc tccgggcacg tcccgataca
     7861 ggcgccgagt ccgtcgcaga agaggtcgct cacgagcctt gcctttccgt cgatgatctg
     7921 gagcgctcct tccgggcagt ccggtatgca gagcccgcat ccggtgcact tctcctggtc
     7981 gatatcgatg atctttctct tcatccgcga ctccctccgt tcggtagatc ttctaaccgg
     8041 ttggcgccca aaaacgtttg cagcccgccg ggacccggga gggacgaggc ataccgggtc
     8101 tgggaggccg ccatccgatg gatactcacc ccatgcgatt cttcaccgga taagaagtca
     8161 tggtaacagc aactctgccg gtgttcggca cctgccgtca acactaccgg gagcagggca
     8221 ggccgggatc agcctgaaat cgtgtacaaa agaggagggg tcggatgcct atccgcgaac
     8281 accggcaccc gcggtgtcct catgtgcttt gaaactctcg ccggcaatga gctcgcgaac
     8341 gctggtcagg atgtcttcaa agaaactgcc gatggaggac agggtcgaca tcacgggttc
     8401 gggaaggagg tcttcagcca cctggccgga ggatccgtct tccgcgatgc cggaatacct
     8461 ctcgatgaca acgactgttc cgtcggttcc gccgccgagc atcgtctcgt ccggctggtc
     8521 gaccagttta aggttggcaa atttcccggg ctgcaccgag accatgtcgc cgggcaggag
     8581 tgtctctccg accgttgccg gaataacatt catttgccgg gttatgtaag ctccgccggc
     8641 gtcaacatac gtgatccggc caaggatagc gtcgtcggtc tgcacctgca gcaccgtccg
     8701 ggaagcatcg atccggtacg ggacatcctg ctcggcggca catgccgcag gtacggcaat
     8761 acctgccatc atgactgccg cgatgaaaag ccagaagcct cggttcatga ccagtttttg
     8821 ctatcggatt atttatcctt tcgcatccgg ccatagagca tatcgcctga gataaccaat
     8881 aatatccatt taaatcgatc acttaatcaa tcaggccccc atcaaggccc cggacatcac
     8941 aggctctccg ctccggaact tcgccggaca cttcccgctc ggttgacgac cccggtatcc
     9001 gggacgaaag caattatcca tggacggaga gattcttaag agaatgtccc ccccgccggt
     9061 ccgccatcct gcccccgcct tcgacgtctt caacgtatac ttcgagcaga tctacgaccc
     9121 gacgatgcac gtcgtcttcg tcttcgacgg cgaggtcgac gccggggcta tgagggaggc
     9181 gacgaagagg ctgatcgcgt ccgaccccta cctccggtcc aggtacgcag aggtggacgg
     9241 ccggcccgtc tgggaggaga tcccggaaga actgtgggaa ggagcgttcg tccttgtccc
     9301 ggccggggag gtcgagaacc tgcccccgcc gccgctcgac gtccgcgccg ggccgcaggt
     9361 gcgggtcggc ctctaccgga gaggggaagg agaccatctg gccgtcacct gccaccacgg
     9421 cttctgcgac gcctccggtg ccatgatcct tgcccgggac ctctttgcga tctaccggga
     9481 gatcctgaag aactcggact tccggccggt cccccgcgag ccctatgacc ggagcacgga
     9541 ccacatcctt gcgctctact ccggggagga gcgggagcgg gcgctcgccg gggaggagcc
     9601 cttcgtcgac cggtggcgct tcccggtcga gcggaccggg agggggacgc ctcgcatcgc
     9661 ctgccggacg ctcccgcccg aacgcctcga acttgcaaaa gcgtttggca aactgcacgg
     9721 ggcgacggtg aacgatgtcc tgatcggcgc gtacttcctc gccctcctga gggtccgggg
     9781 caacccatcg gaccggggag caccgttatc gctcctgact tcggccgacc tgcgcaggaa
     9841 gcaccccggg tgccacggcg agggcctgcc catgaacctc tccatcgcct acgagatcac
     9901 cctctctgca gaagtgagag cggaactggc agatgtcatc gaccaggtga cagccataac
     9961 gacccaccgc aaggcgggaa atcttggcct tgcggccatt ctcttctacg aagagatcct
    10021 ggcaggcggg atgccggcgg tgagagcgtt ctacgacggg atgctcggaa ggtaccaggc
    10081 atccggccag aaaaacccgg tcttctccaa cctcggggtc ttcgaccccg gcgattacct
    10141 tccggtcccc ggaaaggacg gtgcggcgct cgatatccag gacatccaat acctcccctg
    10201 cacctgctgg ccgtatgggt ttctgctgtt agcctccacg ttccgcggcc gcctgacgat
    10261 catgacggcg tacgaggagg ggccctactc gacggccgtt gtggagcagt tccttgagta
    10321 cgtggatgag taccttccgt gagatctgct gcacgcggca ggccggaata gaggcagcac
    10381 cggcagatgc cgcatccctg tcccggggca gactcatacc cattttctcc tgctgcgccg
    10441 atcattgagt tgatcaggac gatgccggac caggtacggg tatctctctg cccggtccgg
    10501 catcgtgcat gccgatgagg gatcgttctc gattgagatc gccgaaccgt tccggcccgc
    10561 ccttctgggc cttgacggat tcagccatat gctcgtgctc tggtggtgcg accgggttga
    10621 tacgaaagag tgccggaacg agactgtttg taaaaaacca tacacgaaag gcccggagat
    10681 gatcgggatc ttcgccaccc ggtcaccggt ccggccaaac ccgatcgcgc tctcggcagt
    10741 tccggtgctc ggcatcgatg ccgccgcggg cgtgatcagg gtcgcctaca tcgacgccga
    10801 cgactgcacg ccggtgctcg atatcaagcc ctacctcccg tgcaccgagc gaatccggga
    10861 cgcaaaggtg ccggcatggt cctcccactg gccggagtgg tacgaagaca accgggggtt
    10921 cgactgggcg gcggagttca cattcgagga atgagcgagc gtatgccggc cggcgcagtc
    10981 tgggtcgtgc atctccggct cttctcaaga agggataccc cgggatcgat gcacgatcct
    11041 gcctgataga ataaaaatcc ttttcccggg tacggttgat gaattactgt gatcgcatgg
    11101 agtgtaagac aacgcgagag ggaaacgtcg tagtggtatc tgcatccgga aggctggacg
    11161 gattcggggc agagaaagta aacgatcttc tgcgctctta cctgcatgaa ccgggatccg
    11221 ggcggtatgt tttattgcag atatccagag atagcagatc catacgaaga acaagagaaa
    11281 aaccgggata tgcaaaacaa taccccggag aggaaacccc actcgatgaa gggaagggca
    11341 cattacctgg cgatactgat aaagcaacgg gggcacccag ggcgagaaac atgccgtcga
    11401 atcagactgg gcatgatttg ataggctccg gaaacccggg gtacgggaac gtcaaaaagt
    11461 atatttcagc gcgaatctca atcactctca ttgaatcgtt actaccctat ttccgaggca
    11521 gagaagcccg gccgctccgt ccgtgtcagc cgaccttctt ttcgtgaatc gttcgtctat
    11581 gaggtgcaat gactcgttcc gctcatgtca tcggctcgga ggcccacaca cttcaccgga
    11641 cctgccgcct tgcaggcggt gttgaaaacg agcttctgac gctccccggc ggtctgatcg
    11701 cctacctggc ggcggtactg cggcaggagc cctgcccgcc gccggagtgc acgcacgacg
    11761 actggaagcg atttctggaa ctgctcgggc cgcactggat cgccgccgtc ctgtattggc
    11821 atctcagcga tctccccgcg gagtgccggc cgccggagac agtcatgacg gccctgcacc
    11881 gcgcctggct gcacgccggg gcacggttgc tcggaacggg caggcagatc gaacgggtga
    11941 tcgcggcgct ggaggcggag ggcatcggag ttctcctcct caaaggcccg gctctcgcac
    12001 gaacggtcta tccggccgca gccatgcggc aggggagcga tatcgacctc ctcatcccgt
    12061 atgacgatat gcaacgctgc gagaatgtca tggcggcgct cggctacgcc tgcccatacc
    12121 gggcggcgga gcatgcaccg cagacggaac accaccagac cttttatccg gaagcggaag
    12181 gggccggccc cttcggcatc gaggtgcact ggaggcttga ctgcgggttc gggctctccc
    12241 cggacgggtt tttggacgag gtcttctccc gctcgatacg ggtcgaggcg gacgacgcga
    12301 ccttctcgac cctctccgtg cccgatcatc tcctctacca ggcgtttcac atggcctgcc
    12361 agcactgcgg cgctacccgc ctgagctgga tatacgacat tgcgatgctt gccggagaga
    12421 tcccggacgc ggccggctgg aaagatctga tccgcctgag cgtggagcga aactgccggc
    12481 tctctctcga agcggctgtt gggatggcgg agttctggac cgggcttacc cttccgccgg
    12541 aggtctcgga taccgctctc tggcctgccc cctcccggga ggaagtagcg gcctgggaac
    12601 tggcgatgag acgctccgtt tcgatcccgg catccttcca gctgaagatg cgtgcgatac
    12661 ccgacgcccg cgggagactg tacttcctct tcaggttcgt cttcccgcac ccggacgcga
    12721 tgcacctcta tcgcaggggg gaagggaagc tgagcctttc ggcggcgtac ctcaggcggt
    12781 ggctgcaggt gttccggtaa cgaaaacgtc cgcgggcttt caggcatcgg tgccgggtcc
    12841 ggggagaatg ccgcagacga tcgtcagcga ccgggtcttt tgctctgctg cgatctgccg
    12901 gatcgccacg gggcgatcga gccccacggc gaagatgcag gcgacgaggc tgcacgcggg
    12961 gcaggggtac atggtgcaga tcttcgccga ttcctcccgg acccggacgc acccggagaa
    13021 gaggcgatac ccacggatct ccacgacgat ctggtcgtcg gagaccgtcg tctcgacgcg
    13081 gtcggcgatc tcaagcacgt cctcgaagac ctcgcggacg gctgtgccga tcgcttcgct
    13141 ctcctccggg acgtgcaggt tgaactcttt ctgcaacgtc gtcagcaacg ttcgcccgga
    13201 aggtacgagg agaataccgg ggcaggcggc gtccgtgagg tacgaatagg tatcgacgat
    13261 ctcgggtatg ccgccgcctt cggtgacggg gacgaagtga accggagttg ctccgtcggc
    13321 ggcgttccgc acgggaatga cgaacccgtt tccggcgatg ccgaggtcgg cggcgtgccg
    13381 ggcggtgctg acgacggcgt ccgcggcaag gagaccggcg atatccgcat cgaccgaatc
    13441 gcccttctgc agggtgacga agaatatccc gacgagaaaa aagaccgcac tgcagagtat
    13501 gacgctggca ctggcaaggt cgctccttgc cgtgaagacc agcaccagca tcagaaccgc
    13561 tgccgtgccg atcaggatca gccccgcggt cgcatgctca ccaaaaggaa tccgcctcat
    13621 gcttcgtcac ccgtccagcg ccagttcagc agagattcgt acagtcccga taccccgacc
    13681 aggatcatgc agcccgcgcc cagctgcagg aggaccggcg ccgtgtggaa cagctggtcg
    13741 gcatacagga cgaatgccga tgccgaaacg ccgaagaccg caaaatgggc atattcgcgg
    13801 acggtccggg gcgaaacggt cattctgagc agcatgccca gaacgaggag ctgcacgacc
    13861 agggcttccc acctgcccgc accggcaacc gccaccacga ccagctcgcc gatgcagagc
    13921 gtgccgaacc accggtgccg cccgtcaccg ggccgcagga tgaggagcac taccaggagg
    13981 cagcagagca ctccggcgta caggagttcg gcggatgcaa gggagagcac cgccaccccc
    14041 ccgacaccca gggccagaac gagcaggcga aggtcgtgct ccatcagagc acctcgactt
    14101 cgaccgggga attcaggcgg tgcagtatcg cctcggccgc tttgggcgta tgaagacgca
    14161 cctttacgcc tctctgttcg gcctggcggc agaccgcatg gaggtggctg agatcgccgt
    14221 cacatgtgct gtagagatcg acgacctgac atttcgcctc ttcgaagatc cgaaacaggc
    14281 ggttgttcag gacgaccggt tcgggcccgt ccagaaacgc ccggtagatc cgcgacagcg
    14341 atccggagaa cgccgcgagg ttccgttcgc cggcgcagct cgagtgcagc cgcccggcct
    14401 cccggcagtg ccgcagagcc gcccggtcgt ccagggcccg gtagaggtgg tgcgtccgct
    14461 cgaccggccc gaggtctgcg acggcatccc agatcgcctc ccggcccaca gcgtgcggaa
    14521 aaaacctgat gatatctccg cccgagaaga ccaggagcga gagatcgcgc cttcggtcca
    14581 attcgctctc gaccctgctc ccgacagcgc cggagacgac ccgcatcgcc tcctcgctcc
    14641 cgtgaatccg gtcgggaagg tcgacgacga tgaagacgga atcctctgcg gtcatggaga
    14701 gttcgcggac gatgtacctg ttgtgccgtg ccgacgcctt ccagtcgatc cgtctcagat
    14761 cgtcgccttc cacgtaagcc ctgagccaga gcgtactctg gccgtgcagc ggtgaggagc
    14821 gggcggactc tgcctcgccg aacccgggag ggtctcgtgg cagaacgggc agcggtgccg
    14881 gaaagatatg gatcgccggc gctcgcgcgc gatcgggagc gtagcgaacg gtcgccgagt
    14941 aaaagaagtc ctcgccgcgg atcgagagtc cgccgaaatc ccggtccccg acggcgagca
    15001 tcttcaggcc gtaggtcgtc cgtgccgcgg gggccgctgc aggatcgatg ctgacggtgt
    15061 tcgtccccga ttcgagcacc gatcctgccg gaatcaggtc acggcaggtg atggagagtc
    15121 tttctgcgga agtgatcgct acctgcgtat gcaccgtaac cgtcgaaccc tgccgggaga
    15181 tagcgggctc gaccgaccgc tcgacggtaa ggccgtcgat gcacgagcga aacgcggaga
    15241 tgaagacggc ataccgcacc gcaaggaaga gggcgagcag gcctgccgcc aggtatatgc
    15301 ccgcatcgtc gagaacgaac gccgctacgc cgaggatgac gatcagaact gcgatccagt
    15361 atgtgggggg tgtcgggtgc ataacttcag ggtacggtta ctgtctgcag gatctcctgg
    15421 atgatctgct cctcggttct cccccggatc ctggcctcgc tccgcagctc gagacggtgc
    15481 cgcagcaccg ctggcgcgat ctccttgaca tcgtccggga tcgcgtaccg gcggttgtcg
    15541 attgctgcaa gcgccttaat ccccttcacg aaggcgatcg aagcgcgtgt gctggcgccc
    15601 accctgacat cggcgtgcgt ccgcgtcgcc atgacaatat cccggatata ggcgagtatg
    15661 agttcgtcga cggtgatcga tctgacgaac cgtaccatga gccgcagggt ttcgacgtcc
    15721 gtatgcgcat tgagattgtc gaagaactcc tgccactcca gctcgccggt gttcacccgc
    15781 cggaggactt cgagttcccc gtcctcgtcc agccgctcca gaacaaggct gaacataaac
    15841 cggtctttct gggagtggat aagcgggaac gtcccttcgc tctcgtgaat attctgtgtg
    15901 gcgatgacga tgaaggggtc gctgacggga acggtgaagc cgtcgatggt cgcctgcttc
    15961 tcgctcatcg cttcgagaaa tgcgctctgc gtcttgggcg tgagccggtt gatctcgtcg
    16021 atcagcagga agttggtata gagcggcccc ctcttcaaga caaaatcccc gcaggagggt
    16081 tcgaatacgc gaatcccgat gatatctgcg ggttgcacgt cgacggcgcc ctgaacgcgc
    16141 tggaaatggc agccggaaaa tttcgatatt gcttttgcta tcgaggtctt cgcagtgccg
    16201 ggagctccct cgagcaggat gtgcccctcg ctgagcaggc agaggtagat catcttgaca
    16261 acgcgccagt tgccgacgaa gtactcggca agctgctcct gtgtctgctc ccagcactcg
    16321 acgacctgct tcagctgctc ctcaatctcc ctcgcgtcca tggaaccctc cgcgcgacat
    16381 ccgctgcgca accgccgaca aaccgatgag aactgtcaaa attaatagtt taaaaatgtt
    16441 cgttttttga attccctctc gaacgtcgat aacgaggccc gagcttccag tcgcagtgtg
    16501 agcctgttcg acggcgaggt gagggcggag cgcgagaagg tttgcgatga agcgggtatt
    16561 gtcgcgcgac gctgcaagct ccgtcatgcc gttgatgaag aggctcccgt ccgcaaggac
    16621 gatcacctcg cccccgccga tcatttcccg ggcgaggacg gcgtgtctgc ccatcgcttc
    16681 gcccttgccg atctgccggt cgccgtcctc gtcgatccac gaaaggagcg tcgaagccgc
    16741 cagagtctcg cctcccgtca gtgcggcagg gcggttgagg aggagcgtat cgacatcctc
    16801 aaggagcggg tattcggcac tgctccgggc aaggatgagg gccggatcct ggtactccct
    16861 gtcgatgctg gagagattcc gcgcgccgat acggatggag gagcccagcg ccgccagcag
    16921 ttcgttgcct gcaaaagact cgtccgctat gaagatggtg ttccccccgg caaggtagtc
    16981 tttcagggac tctatctccg cggggtcgaa cggccggttt ggggcgatga tgaggaggat
    17041 ggtgtcccgt tcgttccgga tcgcggtgca atcctccatt atggcggcgt gatgcgcctc
    17101 gacctgagag aagaatcctg atgtcccgtt ccattcgaca ttgtaccggc tgtactcggc
    17161 gtcggtggtc gtctgctgga agaggagcag cgtcgccgcg atgataacag cgaagagcag
    17221 aacaaaggca agccgattaa tgccgatcac cccggaggtg cgccgtcacg gagtcgaaca
    17281 gttccgttat cgatgcgtac tcgccgtccg ataccgggat tctcccgtat acgtgccgtt
    17341 cgtgcgctgc aacgaacgcc ttgaggtcgc tctccggaac gcgtccgtga agcagcaaaa
    17401 gcacctcccg cggcgtgagg gtatcgggcc ggtccgtgcc gagggttcgt cccgcttctt
    17461 cccggagctg caggaaccac cggtggacgg cctcccgtcc ctccccggac gacgtgggag
    17521 gagtgatggt cgtgcgaacg gcggtgtctg ctacaaccac cggcggttcc tcgtcggggg
    17581 gcaggggttc ttccggtgct tcgtcccgtc cctcatcgtg cgatcttacc gggcgtctcc
    17641 gggaatgccg gaagtagagc gctccgccga gggagacggc ggcgacgagg catgcggtcc
    17701 agagcgtcgt ggtgaggcgc ggggtgagca tattttcgat cacgatctcc tgaatgtcgc
    17761 tctctgaggg gttgagggga agcgagccgg gctcgaactt cgcctggtac gcgtaggtcg
    17821 tcgggtacca gaggtagaac gcccgctcgt accgcccgga ctcgttcgtg gtgatcgttc
    17881 cggcaggaat cgagaagacg tacatatcga cggtggcatt ccgcacggga attctcgtga
    17941 ccgtccggag cgagaccagg aacgttacca accgggagtc gtcctgccgc aggagcgtga
    18001 ggtcgaggac ggtatcctcc gcgacgaccc aaaacgaccc gagatccgag tggtgcaggt
    18061 aatcggtcgc atagaccgta tgaacgcccg ccggtatccg ctcgaccagg aacgcagcgc
    18121 tgaagcatcc ttccgcgtcg gtctcggtgg tgatcgcttc ggtgccgtcg acgaagatcc
    18181 cgatcgaccg gttcgcatgc acggggcctg catgtgtccc tgagatccgg acgacgtcac
    18241 ggtagacagc ggtctccgga gagaccgtaa gcgagatcag gggacgatct tccggagaga
    18301 cggcgctgcc gccggcgccg gcgatcatac gggcggccgc ctcctgctgc tcctcctgat
    18361 accggtcggc cttctccgcg atcgccgcaa cgtcatccac gctctgcagg taccgggtgg
    18421 tatccagatc gaaccggtcg ctgatgtcca ggatctgcga tttctgggat gcgtaccggt
    18481 ccgagagatc gcggatcttc cgacgtagtg cttctccttc gtacaccctc gacgtgatct
    18541 catagggatt ggtcgggtcg ttccaccgga tctccagcct ttcgagttcg ccgaaccgtt
    18601 ccgtcgcgtt tttcagcccg gagagatatt ccaggttctt ccggttgcac gcgaggaacg
    18661 tgtcgatatc ggactcgtcc atctccagtt cgatcacgag cccccggagg tcctcgatgc
    18721 cgtccaggta cgcacgcagt tcctcttcgg atgcgggaat gttgtccgac gagagataca
    18781 gcgtcacagt tccggtcgcg ttgagcagat cacccatcgc ctcgaggaca tcctcctcgc
    18841 gctcctgcac actgtgcttc agtacggcgg gattgaagtg gtcgtccgtg gacgatccgt
    18901 tctcgggggc gtaaaggacc ggtgaggggg cgaccgcgac gaggcagaag agaaagagtg
    18961 ccgtgacgag tgcaaacgcg gcggcgaaga ggaccgatct cataccagaa tgatctccac
    19021 gactttcagt gcaacgatat acccgaagat cgccacgccc gccgcaatga tataccggac
    19081 ccactgcagg taccgtggcc gggagaacac cggatcgacc agtacgacga cgaccaggat
    19141 cccgatcaac cacaggacga agaggatctc aaggttcagg ctccgggcga gaagcatgaa
    19201 ggatactacg agaaagagcc agagggtcag ggcaccgagc gagatcacct tctttccgac
    19261 agccatgagc cagcatcctt gcagtatcga attcgtctgc ggatagatag gatatttatt
    19321 gccgactctt atgacccttt tgctatcgat cccgcgcttg cgacgagggg aattaccgta
    19381 tgcactgcgc tcgtcgacgg agcgtcgccg tcccggtcgt ccggcacggg tgccggcgat
    19441 gcaaggaact cccggatctt cctcttgagc gcctcgccct cgtagatctt cgtattcgtt
    19501 tcgtatgggt tcgattcgtc gagccagttg atctcaaggc gctgcagccg ctcgatccga
    19561 tcgtcgtccg ggaggatcgc gagagcaccc cggttccgcc cggcagcgtc gaggaactgc
    19621 tgcatctcgg gctcggtcat gcagaggttc agcacgacga cgctgacggc gccggaacgc
    19681 tccatgatgc gatcgatctc ggcggcatgc cgcctgtaat cgccggagac gtcttccggg
    19741 gagagagtga cggtgagagt ctcccgcccc ggatcgtacg cgacgccggg cggtgtcgcg
    19801 atctctcccg cgcccgggag gaggagcaga acggcgacga gagccgctgc tgctacggca
    19861 gggacgagcc ggagaaaggg cgtggtccgg tcaggtgcgg gtctcattct tactcaccac
    19921 ggcgtgccga tcgatctgca tcgacaaaag acccatattc gcgcacggaa cgatgaggct
    19981 ttcgcaacga aggcccccgc cgtctttcga atccggcagg gttaaacctt cccggcacca
    20041 tctgttatgt aaatggggcc tgtcttctga aaggaaggct gttcgcaacc gatcgtgttg
    20101 gaaacggagt agcgatgagt ctggccgcga tccacaccga actgaagtac gcactctcgg
    20161 tcggcaggga tgccctgtac cgcaggtgct ccgccaccgg tactctccgg caccgggacc
    20221 tcctcttttt cgccagctat cttcggccgg tggcgggact cggagcactc tcgctgaccc
    20281 tcctcctggt cgcaaccgca ctgcgggcgg tcgtccccct gagcggaaag atcctcgtcg
    20341 acaacattct gctcggatcg gggcccgaga gcctcgaagc gtcactgcag gcactgcacc
    20401 tcggatttct cgcccctctc gccgacctcg ccgccggttc ggtgaccggg ctcgtcgcgg
    20461 cgctctgcgg cgtgatgatc gccatcggca ccataggatt cgtgcagcgg tacgttgcga
    20521 tcaggtttca gcaggaactg tcgttccggc tccacacctc gctcttcgac cacgttctcc
    20581 ggtttccgct ctcctacttc aagaaacatc ataccggcta catcatggcc agggtctccg
    20641 acgacgtgga cgcggtgcag gccctctttg cgcagctgac gttccaggtc ttcaccaatg
    20701 cagctctcct cgtcatctcg ctcgccatcc tcttcatgct caaccccggg ctgacgatcg
    20761 tcatcgcagg cctgtgcccg gtatacgccc ttgcgaacct gctcttccgc aagagggtct
    20821 atgcaaaggc ctatacggag agggaggcca ctgccgacgt aaagcggggg ctgcaggagg
    20881 tgatcgccgg tatcgatctc gtgaagtcct tctcgtccga ggaccgtgag gtcgaacgct
    20941 tctcaggtcg gttgcggaac gtcttcgatc tgcgcctgca acagtcgatc ctgcagaccg
    21001 cctccggctc gtccgtctcc gggctgtact tcctcggctc gctcgtcgtc ctctggttcg
    21061 gcgcagagca ggtgctgcac gggtcgatga cgatgggcga ctttgttgcc ttttttgcct
    21121 atgcctccat gatctccggg gtcgcgatcg cgctcttcac gctccccgct gccatacagc
    21181 ccacaatcgc atctgcaaac cgggtccgcg aactcttctc catggctccc gaagacgggg
    21241 tcggctgcga ccaaagcgcc cttcgccgcc tgcccgaggt aaatggccgg cttgcgttct
    21301 cggacgtatc cttctcctac gattcgcaga agccggtgct cagagacgtc tccttcacgg
    21361 tcaaccccgg tgaaatgatt gccatcagag gcctgagcgg cggcggcaag actaccctga
    21421 tcaaccttgt cctgggatac tacgccccgc agtcgggcaa gataacgctc gacggaacga
    21481 gcctcgctgc cctggacctg cgctggctca ggagcaacat tgccgcggtc gcgcaggaga
    21541 ccttcctctt caacgacacc gtcgggaaca acatccggta cggcaacccg ggcgcgtcgc
    21601 acgccgaggt cgtccgggcg gcaaagctcg cgcatatcca cgatgcaata tcgcgccttc
    21661 ctgcaggcta cgacacgatc gtcggcgagc ggggagcata tctctccgaa gggcagcgcc
    21721 agagaattgc gatcgcccgt gcatttctga aggatgcccc cgtgcttatc ctcgacgagc
    21781 ccacctccgc cctggacgag gagaccgaag ttcggatctg cgggtcattg cgtctcctcg
    21841 cccggggcag gacaacgatc atcacctctc accgcacgcc gctcctggcg acagcggacc
    21901 gcgtctacca cctcgaagag gggagactgg tcccggacga agacttcacg gcgggcgcgg
    21961 aagggtgtcg cgatcgagga tgagcccgaa cggaggggag agacgccatt cccgatcggc
    22021 agggcgcagc agaccgatca tccgccgtcc ggagaagagg tgcagctcgg tcccgtccgc
    22081 ccgtttgaac tcgacgaccc tgaccctgat gaagggtgag aggagacgcc gtacgctatt
    22141 cgggagagcg acgtcccgac atccgcgccg gaggaggtgc cagagcaggc ggcggcgccg
    22201 gtgatacccg cgcaggagaa cggccgacca gtggccgatc cgtccgcctg gcacccggac
    22261 gcgcctcccc ctgcgctcga ccgcgatcac ccgcccttcg acggcatcga tcggtagcgg
    22321 ctgctcgtcg accgacgggt tggcatcgcc gcgggtggtg aagcccatcc cggaaacgcc
    22381 gcagatgcgg tggacgatcc agcgcccgtc cttttcggag cggaagagca cgacgtcgcc
    22441 gcgccggagt tggccgcgcc ggtgggcgga gcagacgacg atgtcgcggt cccggagggt
    22501 cgggtacatg ctccgcccgg tgtacacgac cgtcccgggc agtcgcccct cgccggtcat
    22561 cccgctctca cctcctccgg gggagcagca gccgccatcg tttcccagaa tctcccctcg
    22621 agcgtcgcac gcaggcgaaa ggccgggacg gcggcggcca gagaccacgc gttcagaaac
    22681 acctgccgcc gcagcgccgc tgcaccgccg ggatctccgg cgctgcagag cagatcgcac
    22741 ttgtctgcgg ctgagcggta gagggcggcg gctgcagcgc cgggcgagac ggggaccgct
    22801 tcgtcgacgg ccgactgctc gagaaagaac actcccccga ccgggacatg ccggtcgatc
    22861 cggcgccact ccccgcgctc tccgtcccgg atgtggtcgc tccaggtcgg cagtgcatgg
    22921 gcccggtagc ccgcatcggt cttcacggca aggacgagat cgtcgcacca cgaccgccac
    22981 ggcggtggca cccgtcgggc gcaggtggtc tttcccgtgt ttccacggcc cgcgatcagg
    23041 tagcccgaac cgtccagcga gacgagagcg gcgtggagcg gcaggcctcc cgaccgcagc
    23101 gcgtgcagga agatgccgta cgaaacgccg tccatctgga tgatctccct gatacgtctc
    23161 tcgtgctctt tgtgcttcag ccgaatagac gccccgagat cgctgtcgcg ctcccggtcg
    23221 agcagcccgc agacgaccgc cccgctccgg aagtgatgcc gcagccgcac ccagggaagg
    23281 gcgtactcga tccagccgtc ccccgcaccg cggcggccga aatttctccc ggtctcgcgc
    23341 ccgacgaagc aggtaggagg ggtgcattcc cgcacgcgtt caaggccggt gatggaggca
    23401 aagcgatcgg tccatgcgcc gagttcctcc tcaaagcaga gggtcaggcg ctgcccgtcg
    23461 gcgagggtgc acgcgtagtt ccgggcgagg ggatcggctg tcattggttc gggagtctgc
    23521 aatcggtctt cgggagaacg accctcaccg gatcacggcc agaagtcgag caatccgccg
    23581 ctgcacttcc acttggagat cacgccgaaa ccctgccggc attctccccc gagccaccac
    23641 ccggaagcgc cgtatccgtc cgcacagtcc ggcccccagc cctggtggcc ggggatactc
    23701 ccgcttgcgc acccgccgta cgcagcaatt tccgccccgc cgccgaatgt atccccgtga
    23761 ctgaccgcca gagtgccttc gcgaagcggg aataatcggg gagtctcgta gtttctgcga
    23821 accatggtat ctcatcgttt ttgcaggttt tttccgtctg tcggagcact ttctgcagat
    23881 acgagccccg ctgaccgccc ggtcgggatc aattgaaatt atttacttct agtgtatgat
    23941 ataggttgtg ccggattccg ggtgcacccg tagtgcagga tgaggatttt gagaacagat
    24001 attccgcaaa acccgtctgt tcgagggcag tgtatcccca ccgaaaatat ctccgcggag
    24061 ggggatattt accgaacgca tagtattatc agtccggatt agaaatgaaa accaggagga
    24121 gggcacatac ggtacgattg aggcatttgc ggtggcgttt gccgatacga accgatcgag
    24181 cggagggcaa gaggagatga cgcctgaaac gcccggggca ccaggggcat atccgctcag
    24241 aatggtgtac gtctacctca cgcgcgactg caaccttgcc tgccgccact gctggatagc
    24301 gccgaagtac cagcgttcgg gtgttgccgc caactacctg cccctcgatt tcttcaccgc
    24361 catcctcgac gaggcgcagc agctcggagc tcaggccgtc aaactgaccg gcggcgaacc
    24421 gctcctgcac ccggagatcg gcgccgtcat ctcccgcgtc ggagacttag ggctccgcct
    24481 caccatagag acgaacggca cgctcgtgac gccgcagatc gcagcggata ttgccgcagg
    24541 aaaacagccg ttcgtatcgg tgagcctcga cgggggcgac gccgccgttc acgagggcgt
    24601 gcgcggcgtc cggggctgtt tcgatgcggc gtgcaccggg atcacgctcc tgagcgacgc
    24661 cggcattgca ccccaggtca tcatgacggt catgcgtgcc aaccgggatc acgtcaggca
    24721 cgtcgtgcag cttgccgagt cgctcggtgc gggctccgtc aagttcaata tgctccagcc
    24781 ctccgggagg ggagaggaga tctggcgcga cggcggagcg ctcacggccg gcgagtacct
    24841 cgagctcttc cggtggatcg agaatcatct cgcgcccacg accgcccttc gcctctactg
    24901 cgactacccg ccggcattcc gcccgctcag ccggatattc tgccgggacg gcgacggctg
    24961 cggcgtctgc ggggtccacg ggatcatcgg agtgctctcc gacgggcggt acgcgctctg
    25021 cggcatcggc gagacgacgc cggacctggt gctcggctcc cacccccacg actcgcttgc
    25081 agccgtgtgg agagacgctc ccgtgctcgg tgcggtcagg aagggaattc cgcgcaacct
    25141 gaaaggcatc tgcggcgagt gcgctctgaa ccggctctgc ctcgggagct gcgtggcaca
    25201 gaattactgc cgaagccgcg atcttctcgc gccgttctgg ttctgcgagg aggcggaaaa
    25261 acagggactc tttcctgagt cgcggcggat cggatcggtc cggaacggcg gcgccgatcg
    25321 ttaggccgcg ctttcgttcg ccgtcagcca cgcgtaggtc gcgctccgat cgtgcaggtg
    25381 ggcaacgatg tagttgtacc gcggctggtc tccggtcttt gcggcatcga gaagggcctg
    25441 gtggatggca agcatctcgg cgcacctgcc gtagatcttc ccacccgggg atacgggaat
    25501 cacgcagacg tcctctgccg gtggcatccc tcccggagga acgccgacga ggctcttttc
    25561 cgtggtttcg atgattgcgt atcccgaatc tccaaatgtg gcgtctgggg aggcgacgcc
    25621 gaccgcaacg tggtttgcct cgccgaagta gaagagggcg acgccgtacc ctgcgcggtc
    25681 gagcagcgcc gccaggagaa gactccgatc gtcgcagtcc ccccgtccgt cgacgacggt
    25741 ctcgacgggg taccgcggct gggcatagtc cgacgcatac ggcaggctct gcacgaaagc
    25801 ggtcagcagt tcaacgtact cgtcgtcgtc cagatccgac cctgcccgga tcttctcgaa
    25861 ttccccgaga aggtcggtat agagagcgtc ctgctgagga tctgccatga aggatccgta
    25921 gtagccgacc agccacccgt ccggtcccgt gccggagcgt atcgtcgcca ctttactggc
    25981 attccgtgcc ccgagataaa cgggcctgtc cgcacgcacg ctgacggtcc gcacctcctg
    26041 catgaacgca aagcggaagg tcagctcctc gaaccccccg acgcccggtt ccggaacgat
    26101 tgcaggaatg gcgatgacac cgtccccgcc gaccgtgtct gcagctcctg ctgcctcggc
    26161 catgcagatc accgtgcata gcacacgcag aatcttcatc ggtcggctta cagacatcga
    26221 aacggaaaag gcaggcattg gaatcgatta cctgctattt tatcacacca atatttaaaa
    26281 gttcctcact tggataaaca gataagatca tccttagcga taattagaaa agttatgtac
    26341 tatcgattaa cgagaggtgg gatacctgcc gcggcaccat tgggaaaagt agatttaccc
    26401 tcatatgcaa caggtgaaca tgcccgatga agagaaggtt tattctgcaa tcattggggc
    26461 tttgaaagag aatccgcgtg gcatgtcaat gaagcagatt gccgaatccg tcggtgtcaa
    26521 caggattacc atggcccggt atctcgatac gctgcagatg tcggggcagg tcgagatgga
    26581 gccgcagggt cctgcaaaac tctattacct ctcacacagg gtccctatat ccgcaatgct
    26641 ggatttttcc tcggattttg tcgtcgtcct ggacacacgg cagaagatcg tccaggcgaa
    26701 cgcaaagttc ctgcagctcg tagagaagac gcgcgacgaa ctcgccgacg agcacctcca
    26761 ccggatactc cgcccggatt ccaccgaaca ggatctggat gcggcgatag cccgggcgct
    26821 cgaaggaaaa gaggtcgtcg aagagatcgc gatccggaaa ggagaggaga acctctcgtt
    26881 caggatgaag atcgtgccca cggtcttcac ggacggttcg ccgggcgtga ccctgatcct
    26941 cgacgacgtc acggagtacc agcgttcgct cgacgcccta ctcgagagcg agagaaaata
    27001 ccggctcctt gttgaaaaca tcggggagct gctggtcaat atggaaagac tcgaccagct
    27061 gaacaaccag gtccgaaacc ccctgcagat gatcgtcggg cttgcaggcc tcgagggcgg
    27121 aaagatcgcc gacgtcatct ataaacaggc aaaggagatc gatgcaatcg tgcaggagct
    27181 cgatctcggg tggcgttcgt cggagaacat cagggagttc gtgaagcgtt tttccgctgt
    27241 tccgaaaccg gaataaccgg caggccgaag aaggggagac cgcttttcgt actaaaatac
    27301 cgggttggaa cggccgaaat ccggaaacta tttatttcct tagaaagaaa cttctgcacg
    27361 cgacggagat ccgccgattg cagcatccaa tccgaaccat catcgctttt ggaacatgac
    27421 cgccggaaac atcagaacca tcgggctgct tgccctcatc cttgcagggt gcgtggctgc
    27481 ctgcggcgca gtcactatcg gagaatggcc tgaagagata cgggaaggcg acccgatcca
    27541 cgtcgcgatc gacggtctgg gtgccgggca ggcattcacc acgaggatag agagtgcggt
    27601 gatcgacctc gacggccggc agacgttcga attcagccag aacaatttta ccatgccctt
    27661 caccctgcgg ggcggcggta tcagagtgga ggtgagcagg gcaaaatggg ccgttctgga
    27721 gatctcctat ccggacgggt caacctcggt gacgcgctcc gacccgcccg acagcctgat
    27781 cgtcatccgg aaaaacacca cgatcgccag cggaagatac ggctacatta caatccgggg
    27841 agaggccgaa gacggagagc aacaggtgac cgccagcctg gagctgaacg gcacgacgga
    27901 tgccgccgtc aatacgaccg tgatggactt tacgatcctg gggattgcag aaggtgcgat
    27961 tcccatccgc gccctcgtcg acgcgcagga atatctccag acgatcgtcc gggtggtacg
    28021 cccgacgccg acacctgccc cggacacggg cgacggaagc gacgataccc ggctggcggt
    28081 tacaccgaat gcaacgccga cggcggccgt cgaagcgatc gagaccttcg accctgcagc
    28141 cgcccaggac ggtcttccgg tcgtcatctg gtccaccgac atgcagatgt tcatcgtcct
    28201 cgcacccggc accacggtca ccgcagccgg cggagagcgg atcgatcctg caacggagat
    28261 cgtcctgcag ccgcttacgg gagacggcat cccgggaggt ttcccggagg gcgtcgaacc
    28321 gtacggccgg aacgtctacg gctgcctccc gcagaacata acaacgtccg cgcctttcac
    28381 ggttacgttt tccctgaccg gagatgcgtg gaacgcggta accgggagga atccggtcat
    28441 ccgggagtac gacggcaagc tcgcagcctg gagagaactg cagacagaga ccgatccggc
    28501 gacccgctcg gtctatgccc ggacggagcg gttcgacacg atcggcctct tcgttgcggt
    28561 gcagcccctt ccgacggaag ataccgcgga gacgggagtc ctgccgctgc tcatcctcct
    28621 gtttgccatc ccggcgctgc tggcagtgct gtatctcctc aaaacccgaa gacgggaaaa
    28681 gtgaacgaaa cgaccaccgt ctccggccgc ttattccggg aggcgtccgc cgtcttcgag
    28741 gaatctcttc aggcagccgt cgggggccgg cccatacgcc tccccggtaa gggtgtaggc
    28801 aagagcgggg caccctccgc ggcagtacgg aaggtactcg caccgcctgc aggactcgaa
    28861 cgaggagagc ggaatcgagc tgcgttcccg gagccggtcc agcgcaggat gcatctgcca
    28921 gacccccttc aggctgtccc ggttgatcgt cccgagctcg atatggggca gctgcaggca
    28981 gggaacgaag acgccgtcgg ccctgaccgc cagcttcgag tacggcccgc tgcaggaggc
    29041 cagaaagccg catccgttcc ggaacccggg agcaccgtcc gcccgcatct tttccatatc
    29101 cgtccacatc ctcgcatcgg caagcgggcc ggcctgggcg gagattctgc cgttgtattc
    29161 ctcgtttaac cggagaaggg tttccatggc atagacccga tcctcgacgg tgagctgcac
    29221 gtatgctgca ttcgaccggc acaggcccaa ataggaggcc gcattggtcg aaaacgaggg
    29281 aaggccgagt tcgtcgagca gaaacgctgc aatgccctcc aggttgcgga cgttatgctg
    29341 gtggagcgtc acccgcaccg gcaccggcag gccgtgctcc cgcaaaatcc cgatgccccg
    29401 caccgccttt gcaaaggtcc cgctcccgcg gcaggaatcg tgagtctcgg gccgggaacc
    29461 gtcgatggaa acctgaacgc cgtcacacct gccggttgcg gcaaggtgcg tcgcaatatc
    29521 gtcggtaaca agcgttccgt tcgagaggat ggcgaaccgc atgcggttcg caccgatggc
    29581 gtcgatgatc tcgggaaggt ccctgcgcat gaacgcctcc cccccctcga gggtgacccg
    29641 catcaccgtg cagtcgccca gctcttcgat gaagcaaaac cagtcctccg tgggaagatc
    29701 gcgagtgacg tcgccggcac tggtgaaatg gctgcagtag gcacatttca ggttgcaccg
    29761 gttcgtgatt gcgatgtcga cggatctcgg ggtcctcggt atgcggatca ttgccggttc
    29821 acttcaaaac ccgcataccc cctgtccacg aggtcatcga gaaatgctgc gacctcgtcg
    29881 gagacggtct cgggcacgtc gtcaaaggcc gatcgcaggc cggcgatgag ttctgcgaac
    29941 gtttttgaac cgtccaattc cttccagaca tgtgcaccaa ccggattgac gccgtaggat
    30001 tcgccggtgt cggggtcgaa gaggagcgcc cagtcgtcga actcttcccg aaagacgata
    30061 gaggggtttg caacgggcag ccggctgtac tccatactac gtcaacaacg agtcctccta
    30121 taaatttgcc gatccgggga tgccgcactg ccgcgtccga caggattaca aaaggaaata
    30181 gcatacgtat ccgccgagtg gaatactcca tgcacagacc atcacccggt tccggcggca
    30241 tggccctgcg acccgtcgcg aacgtccgga ataccgtaca ggaacccttc ctcgtcgccg
    30301 gcagccccat catcgacatc aagtcctacg tcccggcccg gttcccaaag ggcccgcccc
    30361 cgccgacgcg gcgcctttga gcgcatgttc gagcagcgtc gccgtgttcc atgtcttccg
    30421 tggacttccg ttgacgccaa ggaccgttgt catgtctcgc cactccaccc accgcacctt
    30481 ccgatcgagg tccgggtcac gccgtgccgg aactgtgggc acctcatcgg ggtactccag
    30541 cggcgtgacg gcaacgatct ccttttcagc agggatatcg aggatcgtgc gcagcgactg
    30601 ctcgtcctgt agcggcccgg tcatccagca ggtgccgagc ccgagcgcgg tcgcggcgag
    30661 cagcaggttc tccgtcgcgg cactcgcgct ctcgatgttc gccctgcgga agttcggggc
    30721 gtcggccgca tcggtcagga cgacgatcgc cgccggtgga tcgcctcaaa gacgtccata
    30781 ttcacgcacg ctccttaccg ccacgccacc tgcaccgggt tgcggcgggg gatgcccggg
    30841 ggtgcgtgtt gcgggttgcc aaagagcatc gcgtaggcga acttccgacc tgtggggatg
    30901 ccgaattcct tctggagcgg ctcgtaggat gcggcggcca tcgagacgaa cccggcccag
    30961 caggtgccga tgccgaacgc cggagcggcg acatcgaagt gcgtcatcgc gatgatcgcg
    31021 tccacggacg cgaccgggtt cccttccggg atatgggcga cgagaaggtg tggggcgccg
    31081 cggcagatga cgtcgttgcc cgcctcccag gcacggatga gccccggcac gtagccgctc
    31141 atcggatggg tgctctccac cagacccttc atccacgcga tcgtcagccc ggcaaccttc
    31201 cggaccttct ccgggtcgtg gacgacgatc caccggaccg gctgtccgtt tcctcccgac
    31261 ggtgcatacc ggatgacgtc gaggaccgcg aggatcgtct cccggggaac gggctcatcg
    31321 gtgtagcggc gcaccgaccg gcgcttccgc aggtagacgc cgagatcgcc gggcgggagc
    31381 gtgcccgcac cggccgggac atccaccttc tcctccggcc tgacgttcag ggtaagcgcc
    31441 tgcgccgggc agaaagcctc gcagtggccg caggcgacgc agatgccggc cttctcctca
    31501 gggacccccg ggagggtgtc ttcgtccgcg gcaccgacga ttgccgtcgg gcagacggcc
    31561 gagcagaccc cgcagcgggt gcagagggag cttcccggcg tcaacgcgaa gatgctcacc
    31621 cggcagctcc gggaactgga gggggacggg gtcgtgcggc ggacggtcta ccccgaggtg
    31681 ccgccccggg tggagtacgc cgtcaccgag ttcggcagga ccctcctccc gatcatggag
    31741 gcgctctgcg cctggggaac gcagtacctc gggatagacg acgcggccgc gccggggtgc
    31801 ccggcgaagg tggggagaga gaaggcatga tcgcaccggc gcctcacgtc gagtaccgca
    31861 ccacggacat cgccggcatc gaggttatcc gcccgctctg ggccggactg aacgcccacc
    31921 accgtgccca tgcccgggcg ttccgggacc tgtacgagcg gataaccttc gacgaccgga
    31981 aggcgtactt cgccggggtg gcggcggtcg gggccctgcg gatcgatctc gccttcgatc
    32041 cgacggcgga gaagtgcgtg ggctactgca tcagttcgct ctcgccggag tcggcgggcg
    32101 agatcgagtc cgtctatgtc gacgaggcat accgctcgca gggtgtcggc acggcactcg
    32161 tccgccgggc acttgcgtgg cttgacgcga acggttcggc cgtaaaccgg gtctcggtcg
    32221 ccgacgggaa cgaggaggcc ttcccgttct accggaagtt cgggttctac ccccgccgaa
    32281 cggtactcga gcaggtcggg agagtgcacc cccgcggccg gccggagaca cgagatcccc
    32341 tggtgtggtg tgcagtatac gggagatgtg cgccgccgac cgggaagaga tccgccggat
    32401 ctatctcgag gggatcgcca ccggcaacgc gacgtttgag tcggaggcac cttcatggga
    32461 gcgctgggat gcgggccatg ttcggtcctg ccgcctcgtt gcggccaatg gccggagggt
    32521 agttggctgg gcggcgctca gtccctcttc cgggcgcccg gcctacgccg gtgtcgcgga
    32581 ggtcagcatc tacgtcgaga acgatgcccg ggtgctgggg gtcgggagcg cgctcttatc
    32641 cgcactcatc gccgcatcgg agcaggaagg gttctggacg ctccaggcag gcatcttccc
    32701 ggagaacgaa gcgagcctta ccctccatgc aaaggacggc ttccgcgtcg tcggccgccg
    32761 cgaacgtccg ggccggatga aggacggcag atggcgggac gtactgctta tcgagcggag
    32821 gagcaggaag ataggcctct gacccgcccg ccggacccga tcgattcgga ttcaggcgct
    32881 tttgccgctg ccggtgctcc ggtacagctc cgatcctccc aaacagcctg ccacatcgct
    32941 tatccctcat cccttcgtag atctccacat ggacccgatc atcgaagcga tacggcagga
    33001 gttcgcggcg cacgcggacc cggtgatccg ggagaatggt caacggttct tcaaggagga
    33061 ggtccggtgc tacgggatga agacggcaac cggaatcgcg attgtaaagg agtactggaa
    33121 ggaggtcaaa tcccgcgaga aagaggagat cttcgccctc tgcgaggaac tctattcgtc
    33181 ggggatgatg gaggaggcgt tcgtcgtctc cgaatgggct ccgcggctcg ccgaccgcta
    33241 cgagcccggc gatatcgccg tcttccggca ctggatcgag acctatatca ccaactgggc
    33301 cacctgcgac ggcctctgca accacgccgt cggggacttc atcgtgcggt acccggagaa
    33361 gatcgaggaa ctgaaacgct ggacgcagtc ggagaaccgc tggatgcggc gggcggcggc
    33421 ggtctcgctg atcgtgcctg cgaagcgcgg agagttcctc gccgaagccg tcgagattgc
    33481 cgatctcctc ctgaccgaca cggatgacct ggtgcagaaa ggctacggat ggctcttaaa
    33541 agaggcgagc agaaagcaca cggacgaggt cttctcctac gtcatggcaa acaagaggga
    33601 gatgccccgg acggcgctcc ggtacgcgat cgagttgatg ccgaaggacc tgaaggccga
    33661 ggcgatgaaa aaagattggt gaggggtggc gaggctcaga acctgacccc gggagcctcg
    33721 gcttcccgca tgttgtactc cagggtccag aagtacgcct cgccgtgggc cacgcggaag
    33781 acggctagtt tggggtcgtc tgcaccggag accccgatct ggaggagcca cggccgctct
    33841 ttgacaagcc gctcttttaa ggccgcgtcg tcgacaaact cgatcgctcc cgcaatccgc
    33901 atcatcattc ctttaccctc gggattgtag aaacagagct cgaccttcgg gttcttggtg
    33961 agctgctgcc agacggcctt cggggttccg gtatggtaat aaaacccggt cgcatcggca
    34021 aaccacatgg caaacgcccg gacccggggc tggtcgccgt ccatcgtcgc gatgtaggtc
    34081 acggggttgg cgtttgcgaa cttcacgcat tcggaaaagt ccattctcct tcctcatgca
    34141 tcaatacgga gaggaagtaa aaaatgtttc cgggctgcct caggcggaat ccggcgggaa
    34201 tacctcgcgg gccagcctcc gcgcctcgtc gagccaggcc ctgcgctgcc cgtcggtgct
    34261 cgtgaccaca acggagaaca tccggcgatg aacggccgga attccgcaga acgtgcagat
    34321 acagtccttc cagagccgtt caaggggatc gccgaagacc tcgagttccc tctgccttgg
    34381 ggtgttcgag gtaatgaata aattttacat ctcataacgt gaagctgaat gtgatgaaga
    34441 gcccttttca cgtcatgatt atccccacgc tcggttgccc atccagatgc cactactgct
    34501 ggagttctga tgaaaggtct ccgaagatga gtatcgagac cgtccgggag ataacggagt
    34561 ggctcaagga ctaccagtcc aaccagatca ccatcacctt ccacggggga gagccgctcc
    34621 ttgcaggcgc ggacttttac cggcaggctc tccccatcct ctccgagggg ctcgcccatc
    34681 tcgaaccgac gttcgccctg cagagcaacc tctggaagct caccccggag atggcaaagg
    34741 tttttgcaga ataccatatt cccatcggct ccagcatcga cggccccgag gagatcaacg
    34801 acctgcagag aggaaagggc tactttgaaa agaccatgcg gggctacgag atagcacgag
    34861 aaaacggact tgaggtcagg ttcatctgca cgttcacctg ccggtcggtc gagcataagg
    34921 aggagatctt caacttcttc ctgcagaggg ggttcccgct caaactgcac ccggcgctgc
    34981 cgtcgctccg gagcgatgac ccgaaagagt gggcgctcga gccggaggcc tacggggaac
    35041 tgctggtcta cctcctcgat aagtacctgg agaacctggg caacatcgag gtcatgaaca
    35101 tcaacgacct ctgcaggtgc atctttaccc ggcacggcac cgtctgcacg ttcgtggact
    35161 gcatgggaag cacccttgcc ttcgggccgg acgggagcat atacccctgc taccgctttg
    35221 tcgggatgcc ggaatacgtg atgggcaacg tctacgaccg tcccacccgc gaagacctcg
    35281 cccaatcaga cgcctggaaa ctcatgcacg cgttcaagga gtacgtcgac cgcgagtgcg
    35341 gagaatgccc ccatattaag tactgccggg gcgggtgccc ctacaacgcg atagcgccca
    35401 cggacggcga gatccgggcc gtcgatcctc actgtatcgc gtacaagcgg atatttgacg
    35461 agatcaccga ccggctgaat aacgagatgt acggttctcc ggatatggag ataggcgggt
    35521 tcggttcgcc attcgggcgg aagaaaaaac ccgggatcat gtcgatcttg caggcgatgg
    35581 cgacgaaatg agctccccga ctaccctttc ttttgtcagg atcagccccg tggcgcgtta
    35641 acgtcgttaa cagcggctcc ggacactgca cgaagcccgg tacgaccgga cctgtacatg
    35701 aactatcagg gaggagacgc tatcccctta ccatgcgtgg aaccgatctc acggacagca
    35761 gccggacgac tgcagggata ctgctctttc tgctcccggc ccagttcata atcgctcttg
    35821 tggccggcgc ggccatcgcg cccgggtata gcatcaacac gaatgcgatc agcgacctcg
    35881 gcgtgatcga cacgacggcg tggctcttca acgcctcccc ttcatggcag gcctcctgaa
    35941 tatcgtcggc ggcggtgatg cggcagtcag ggcaggttcg ggcaagttcg accgtctgca
    36001 tcccggcccc gcacccgata tccaggatct tcggccgggc cgggaggtcg gcgagcatcg
    36061 agaaagcttt ccgggtgcac tcgttgctcc ccggccccct ggcggggcag gccctcgtgc
    36121 atggtaaaaa tgaaaggggt ttccatgaaa gtaatgcgtc gcgcggctct ataacctata
    36181 cggtcggaat tgcttccaaa cgtcgcgaga ctgcgtccat gccgaacccg gggttatagg
    36241 gaaggattac aggcccacca tccccatgac cgggaggggg atcggggcgg ccccaactgc
    36301 caaaacccgc atcccgccag agctaaatat catcgccgcc caatcatgcg tatggaacga
    36361 aagacccccg ccaccgaact ccgggaccgt atggagcggt tccggctccg tatggacgcg
    36421 gagaacccgt catgggagtt tgccgcaatc tttggcaggg taaaccagtt ctatttcacc
    36481 gggacgatgc aggacggggt tcttctgatc ccccgcggcg acgaggccgt gctctgggtg
    36541 cgccggagtc tcgagcgggc gctcgacgag tcgctcttcc ccgatatccg gccgatgaag
    36601 agtttccgcg atgcggcatg cgggatgggc gcgtgtccgg cgacggttca cgtggagacg
    36661 gagacggtgc ccctcgcact gctcgagcgg ttccggaagt atttcccctt cacatgcgac
    36721 gggcaaggag gcccccggtt tcaaccgggg gaggaat
//
LOCUS       DAJB01000191           13613 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_44657, whole genome shotgun sequence.
ACCESSION   DAJB01000191 DAJB01000000
VERSION     DAJB01000191.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13613)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_44657"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    6806..6908
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12628..12659
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2478 a         4184 c         4244 g         2570 t
ORIGIN      
        1 atagagttaa atgacaacga gtactcctct tttggccggt cgatctctgt gccgggacgc
       61 agtatgtcac cgaagaacgc cggcaatgcg ggatgcatgt ggctgaggcg gctgcatcac
      121 ccggaccaaa aaagtattgt cggcctcgtg cagacccggg gcccgctaca gcgtctcctc
      181 gccgtagaga tacggccgga gggcctccgg gacggtgacc gaaccgtcct cgttctgata
      241 gttctcgagg atcgcccgga tagcgcgcga cgtcgcgatc gcggtgctgt tcaaggtgtg
      301 cacgaagcgc ttcgaggtga attcaacggg gtcgcgcacc ttgatgttca gcctgaccgc
      361 ctggtatgcc gtgcagttcg agcacgacac cgcttcgcgg tagcgctcct cgcgcggcat
      421 ccagacctcg aggtcgtact tctttgcggc gacggtcccg atatcccccg tgcagatcga
      481 gacgatccga taggggaggc cgagttgctg gaagacttcc tcggcgttcg ccatcagttc
      541 ctcatggagg tcccaggact gctccggcgt ggcgtagatg aactgctcca ccttgtggaa
      601 ctggtggacg cggaagagcc ccttcgtatc gagcccgtgc gccccgatct cccgccggaa
      661 gcacgggctc agccccnnct cgtcgcgatc aggaactcgt cctcgccgtc gatcttgtac
      721 atgacgttct cgaagtcggc aaggtcggtc acgccctcgt atgcggcccg gttcagcatg
      781 tacgggggga tcaccggggt atacccgcgc tggacgagga ggtccatcgc aaaccgctgg
      841 agcgccatgt cgagcagggc gagcctgcct tttaagaagt aaaatccgga cccggatatc
      901 ttcgcggcgc gctcgaagtc cgcccagccc tgctcgacgg cgagcgcgcc gtggttcttc
      961 aggtcgaacg cagggatctc cggtttgccc caccgccgga tctcgacgtt ctcggtatcg
     1021 tccttgccga tgggcacgct ctcgtgcaga atgttcggga gccgcatcag gcggtacttc
     1081 actgcttcgg tgagtttatc gtactccgtc tccgcctcgt tgacccgcgc gggaaggtct
     1141 gccgcctcgg agaggagccc ggcgatatct tcccccgcct tcctcgcctg gctgatctcg
     1201 cgggatatga cgtttctccg gttgcgcagg cccccgatct ccacggtgag ttcccgtgcc
     1261 ctccggtcca tctccagcac ctcgtcgacc caggccagtt tctcggtatc tcctctcttc
     1321 gtgaggtctg ccctgacgat atcggggtgt gcacgaacga acttcaactc aagcatactg
     1381 tctctctgta cgtacttcgc catgacggta tatggtcatg ttgccccctc ctgcgggatg
     1441 agtccgggcg actcccgggg cggagcgccc ggaccatgtc ctcttgcccc cgactaaaac
     1501 ctactcttat aatccccggc gaaccaaccc atgtagcatg gaaaagaacc agaagatcgt
     1561 tctggtgatt ggcgccctca ttacgctcgg gctggtcttc atcaacacct tcttcgccct
     1621 catcgcgctg atcatcgtgc tcgtcctcct gatgagcttc cgcatcatgg gagattccgg
     1681 caactacccg ctcgtcgccg tggagctctc ggaagatgcg cgggagatta tagtcaccaa
     1741 tacgggcacc gcgagggcgc agaacatcca cgtcgctctc gtcccgctcg acatcgattt
     1801 cgagatcgcc tcgctcgacc ctgacgaaga atcaggattt ggcctcgctt cgatgctcgt
     1861 ggaggcaaaa gcggtcgtca cgtacgaaaa tgacgaaggg cagcagttta cgcgctcata
     1921 tccgctcacg gcgctcgggg ccgggcgtga cctcctggaa ccggtgttcc cgatcttcgg
     1981 gcgccggtga ggagacagaa gcgggggagg ctcccgccgg gcacttccct cagtcctgcc
     2041 gctcccaaac ctcaggttct aacactgatg gctgtgagtc acagccacgg gaacaatgta
     2101 aaaattggct ctcctgaaac cccattctgg tcttcctccc cctcctgatg aggctggtgc
     2161 cggatccggc ttgtccccgg gacgaccttt ttttctcggg tatctcctca tgcagtagac
     2221 cgtggtggct atcgccattg ggggtctcgc cctccctgtc tcgcgcgaag atccgcatac
     2281 ctcatcgatc ccactcctcc cgcgcttcgc gctcctcccc cgcccatggg cgggggcagt
     2341 catggcgata cccggtggaa agccgtgccc ctgattgcgg ccatcttgaa caaacaggtg
     2401 gggttcaaca aagccaaaaa ttaatttttc aggagccgag gtactcgagc gtccgtgcga
     2461 gcgcggccgt cagttcctct tctttctttg catactcgta gagcgcccgg tagagcatga
     2521 agacaaggtc gtagccgtag acgtcgaagg cctcctccgg cgtcagggag cccagatacg
     2581 aatcggtccg gatctcggtg gtggtgtaca tcaactcgtg gaaggccccg tcttcgtcga
     2641 ggacgcatat ctgtgcatcg acgggcatgc ccggatcgtc cgggcggtac gggagcgggt
     2701 cgcccttccc gaggacgatc atcttcttct cataaaacac ctgttcgtag agctcccccg
     2761 atgcctccct cttcgcgcgc cggagcatct ccagcccgat ccgcttcacg accggcgccg
     2821 tatcggcagc catccgcgca aggagggttc ccacgttgcc ccggagttcc gaggccgact
     2881 cttccttctt cgcctgcagg tccccgaggg tctcgagaag ccgggcgtat ccctcttcga
     2941 tgatcggatc cattgttcac tcacctcttg cgtacgccgg cataagcaca ccggtttgtc
     3001 ccgctagtcg ccctgccgga ggcgctcgat gccgagagcg cccgcatacg cgagcgtgct
     3061 cacgagaatg atcgtcgccc ccgaggggac gttcaaaagg taggagagcc atattccggt
     3121 cacggagaag acgatgccga gaccgacggc aagaagcatc atgctccaga tccgggtggt
     3181 gaagaggcgg ctgatcgccg ccgggagcgt gaggagggcg atcacgagga tgatccccac
     3241 cacctggatc agcatcacca ccgtgagcgc aatcagcacc aggaggagga gcgagagccg
     3301 ttcgaccggg aggttcatga ccgtcgcgta atccgggtcg aaggtgatcg cctggaactc
     3361 ccggtagagg taggctacga cggcgacgat gatgacaatg aggatcgcca tcagcaggat
     3421 atcgtcccgc ggcacgagga ggatgttccc gaagaggtat gagaaaaggt cgggggcgaa
     3481 tcccggtgtt aagtagacaa agaggatgcc gagcgccatt cccgccgccc agaccgcgcc
     3541 gatgagcgta tccatctgct gcctggcccg gagctggagc gtacccatcc cgagcgctgt
     3601 cgccacggtg aacccggtgg cgccgatgag cgggtctatc ccgaggaagt agccaaggcc
     3661 tatcccgccg aaggctgcgt gggagatgcc gccgctgacc gagaccatcc gccgcaccac
     3721 gacgtaggtg ccgatgacgc cgcaggcgac gctcgcgagc accccggcga cgagcgcgtt
     3781 cctgaagaac tcaaatccga gcacctcgaa catgctcact cctccctgga gtgttccgaa
     3841 aagacgcggt gcggaacgcc gtgggcgatc aggtccaccg gacagtggta tgcagcctca
     3901 agcatatccg gcgtgatctc gttcgtatcg tgcgtataga ggcgccggtt taagcaggca
     3961 acccgggtca cgtgggccgg gatcaccccg atgtcgtggg ttacgaggac gatcgccatc
     4021 cggtcgcgga ggcggtccag gacctcgtag aactgcgcct ccgtcggggc gtccacgtag
     4081 accgtcggct cgtcgaggag gagcaccttc gggtcgccga cgagcgcccg ggcgatgatc
     4141 gcccgctgct gctccccacc cgagaggtcc cggatctgcc ggtcggcgag atcggcgatg
     4201 tgcatcgtct caagcgcctc ctccgttcgg tcacggtccc cggcatcgta gcgccggggc
     4261 ctgcgggtga cgtggccgag gcggccggag aggaccatct cccgcacggt gatggggtaa
     4321 tcgaagtcga acgtccggaa ctgggggacg tagccgagga acctgcgcat ctctgccccg
     4381 gtgctgccga ggatccggac gacgccgcga gagggggcga gcaggccgag gatcacccgg
     4441 aggagcgtcg tcttcccgcc gccgttcggc ccgatgatcg cgtagaactc gtcagggtga
     4501 atggcgaggt tcacggcctc aagcaccgtg tggccacgca gcctgaccca gacatcgtcg
     4561 atctcgatca cgggggcgct catggtttgc cgctctctgc gaatgccgaa gccacgtctc
     4621 gcatgttctc caggtagtcc ttcgcgagcg ggctcaccag caccacggtg ccgtcgatct
     4681 cgtctgcgat cacctcggcg ctccgggtgg agtgctcggg cgacgcgaag atcactctga
     4741 tctgctcctc cttcgcctgc gtgatcaggt gctctaacct ctgcggggag ggttcttttc
     4801 cctcgtcctc gatcgggacc tcgacaagac catagtcccg ggccaggtag gcccacgacg
     4861 agtggtagac catgaccttc gtggtccctg attcagcgag cgctccggcg atctcagcgt
     4921 ccagggtatc aagttcctcc aggtaatcat cggcgttccg gcggtagtcg tcggcgtttg
     4981 ccgggtcgac ctccacgagg ccttcgcgga tgttctcgac catgaccttc gcgtttttgg
     5041 gcgataccca gatgtgcgga tcggtccccc cgtggtggtg gctctctagg gggtcttcct
     5101 cctcgtgatc cgcctccccg gtcgagatca ggtcgacgcc ctgcgagcag tcgacgacga
     5161 gcatatcggg gttcagggcg gcaatcttct cctgccaggc aagttcgaac tctatccccg
     5221 aacccaccac ggcatacata tccgcctccg cgacatcggc gaggaccccc ggcggcggtt
     5281 cgtaggtgtg cgggtcggcc ccgggcggca ccagcagaat cacgcggacg tgatcgcccc
     5341 cgattcgttc cacgaactcc tgctcgggcg gtatggtgac cgcgacgacg acccggtcat
     5401 cctgctgccg gtcggtcccg gtgcatcccg ccgtggctgc tgccagcagg aggattacaa
     5461 atgcggctgc aatgagcgat aaagaagtaa tattgccttt atcctgtgat cccatggttc
     5521 tccactccaa atgtggtatg gccccaaatc tcctcatgcc gaaataaaaa acgttttacc
     5581 gtcacctctg gtggtcgccg gcggggcaac agcagcaatg gtcgtgctcg atctcgccgc
     5641 agccgctcat catcgggtgc ccaagcgacg tacacatcct gttggtgagg tcctttgaga
     5701 agcactgctc gatcttcttc gcctccctgc aggcttcgtc gggctcaagg ccgtagcggc
     5761 tgagcaccag ggcgacgatc cgatgacgcc ggatcaggaa ccgggcgtag tcggtgccga
     5821 gagttgtcat cttcacccca tgatagggcg tatgctcgat gtatcctgct tttgcaatct
     5881 ctccaagcgc ctttgtcgcc gtcgagggcg cgaccgagaa gtgggcggcg atctcggtcg
     5941 tcttcacgac gtcgccccgc atatagatgt atttgaggaa ttctgccttc ctcgaggaga
     6001 gctcatctcc ggtgaacccc tgcatgggaa gagggtatgc tctgccggaa gaaaaagttt
     6061 cgggggggct aaaccctgtg gccggcaccg gacgggacca gcgtgccgat gacgtagtgg
     6121 cgccggttgg cggtgatcat cgccctcccg gagaacccga acggcaggtc ttctgcgatg
     6181 acgacgttga ggtagcaggg gttgcggcag acggtcgagg aggtcgcgtg cttctcagtc
     6241 gcaacgattg gtgtctccct cccgatccag cgctcgttgt agcggtcgta gatgatgttc
     6301 gcctggcgaa gtagcgcccg ggacctgact ttccgtacct tttcagggag gttcttcagg
     6361 acagcggcgg gcgtgcctgg ccgccgggag tagcgggtga tgttcacctt cacgaacgcc
     6421 gcccttcgca ggagttcgag cgtctgttgg aactcatcgt ccgtctcccc gggaaacccg
     6481 gtgatgaagt ccgacgagat catcatatcc gggtagcgct cccggaacgc atcgacgatc
     6541 ttcacgacgt cggccgcgga gtaaccgcgc tgcatccggt cgaggacgga gtccgaacct
     6601 gactggacgg ggaggtggag gaaccggaag accttatcgc tctcgtaggc atcgacgagc
     6661 ggttcgagga tcctgagcac cgatgccggg ttcatcatcc ccagccggac ggcgaatctc
     6721 ccggggatac ctgcgatctc ttgcaggagg tcgggaaggg tctcgccccg gtcgagcccc
     6781 caggcagcca cgtcctgccc ggtcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnngt gaatctcgtc ttggtggatg atctgcggtg tacagaccgc gtgaatcttc
     6961 tccatctgga cgagcggcat gcacccggtc acgtagaggt cacggtcggc gaaggccgcg
     7021 aggcgccgga gcattttccg ttcggttgct tcgatcaccg tgcaggtgtt gatgatcacg
     7081 gtctccgcct cgtccggccc ggtcagcctg cagccgagac cttccagtat cgcttccagc
     7141 ctccgggtgt cggcgtggtt ataggtgcat ccgtaactct cgatgtagat tcgtcttccc
     7201 cgcagatcct caagggtaat agcaatcgac tccctgcgcg cggtcggcag ccggaccgat
     7261 cccgcggggg tgtgatcccc cgtatcgttc ggaggatgtt ttaccatctt ccggccggtt
     7321 ttgtgccgag cacaatactt aaccgctgat attacaacct ctttatttaa tgattaaaat
     7381 agggttgctg ggatgcggca acgtcgggca tatcatcgcc acacacgccg agagcatcca
     7441 ggttgtcgcc gtcttcgata tcatccccgg acgggctgaa gaactggcgg cgctctgcca
     7501 tgcgcggccg tatacggact ttgaagcctt catgcgggat gacttctcga tcgtcgtgga
     7561 agccgcctcg gtcgatgccg tcaggttcta tggcgaggcg gttctccggt cgggacggga
     7621 catcatcgtc ctctcagggg gagctctcgc ggacgatgag ttccgcgagc acctcattga
     7681 agtcgcacgg gaggcgggaa agaagatccg tattccgagc ggcgccatca tcgggctcga
     7741 taacctcaag atcggtcagg tctcgccgcc gaggaaactc ctcctgcgaa cgacgaaacc
     7801 ccccgcgtcg ctcgggatga ccgccgcggc ccctacggag atatttaagg ggctggcgca
     7861 cgactgtatc aagcaatacc cgaaaaatat caatgtcgca gtagcgttgg gactcgctgc
     7921 cggcagggac gccgacgtgg aactctgggt cgaccctgca gcggagagga acattcacga
     7981 gatattcgtc gagggcgact tcggggacat ctacgtccgg gtcaggaacg tcccgagccc
     8041 cgataacccg gcaacgagtt acatggcagc cctctccatc ctgacgctct taaagaacct
     8101 cgagaaccct ctggtagtgg gtacgtagca atggaaaagg agattcgtgc gctcaaagcc
     8161 agaaagaacg cagtcgtcat ggcgcataac tatcagccca tggaggttca ggcgcttgcc
     8221 gacgtggtgg gcgacagcct cgagctcgcg gtgaaggcga aggagaccgg ggcggacctg
     8281 atcgtcgtct gcggtgtccg gttcatggcc gagacggcaa agatcctcaa cccggagcgg
     8341 aaggtgatca ttccggtaga ggacgcgggg tgccctctcg ccgacttcct gaccccggac
     8401 ctgatccggg aggcacgaca gcgatacccc gatgcggccg tcgtggtcta cgtcaacagt
     8461 acagcggaaa gcaaggcgct cgccgacatc acctgcacct cggcaaacgc agttcaggtc
     8521 gtcgcgtcgc tcccaaacga tacgatactc ttcgggccgg atgcaaacct tgcggcatat
     8581 gtccagcggg agctcccgga gaagacgatc gtcccgatgc cgcccggtgg ccactgctat
     8641 gtccacaccg ggtttaccct tgccgacgtt gaagcggcgc ggagaaaggg cggcagggtc
     8701 atctgccacc ccgaatgccc gccggaggtc caggagcggg cagacctgat agcctccacg
     8761 ggaggcatgg tccggggcgc cgcggccggc ggggacgagc cctggtgggt cttcaccgag
     8821 cgggagatgg ccttccgcct ccggatgctc tacccgggaa gggtctttta cgagaagccg
     8881 gaggctgtct gcgcggatat gaagaagata tcccccgccg acctccggcg ggcgctcgag
     8941 cgcgaggagt atgaggtcgt cctctccccg gagatcatgg accgggcacg gcgggccatc
     9001 gagcggatga tcaccgtcgg agcgtgagta tgatcccgat tgaggacctg ctcaggttcg
     9061 tccgggagga tgcgccgtgg ggcgacgtca cctctgagac ggtcgtcccc gacgtcatct
     9121 gccgggcggt gatccgggcg aaggatgcag gggtcgtcgc aggcctcgcg gaggcacggg
     9181 cgctcttcga gcacttcggg gtcacggtcc ggcagcactc cgctgacggc agggcggttg
     9241 cccctggagc gaccctcttt gagctcgacg gccctgcgag agcgatcctc ctcgtggagc
     9301 ggacggcgct caacatcatc gggcggatga gcgggattgc gacccggacc cgggaggcgg
     9361 tcgacgcggt ccggaaggtc tcgcctgccg tccggattgc cgcgacccga aagaccgccc
     9421 cgggactccg gatgctcgac aagaaggcag tggtgctcgg cgggggcgac ccgcaccgct
     9481 actgcctttc ggatatgatc ctgatcaagg ataaccacct cgcgctggtg ccgctccccg
     9541 aagcgatccg gaaggcgaag cagcagagcc tctaccggac ggtcgaggtg gaggtcgaga
     9601 cggcacagga cgcaatcctg gctgcagagg ccggggccga tatcattttg ctcgacaaca
     9661 tggcgccgga cgcagtccgg gagaccgtcc gggtgctcaa cgagcggggc ctccgggacc
     9721 acctgaccct tgaagtctca ggaggagttg gcgccggtga ccttgccggg tatgccgcga
     9781 ccgggatcga tatcatcagt atgggtgcgc tcacccatac ggtcaggaac ttcgacgtga
     9841 gtctggatat tctgaagggc gcgggcacag tccgggttcc ctgaccggtg ccgcccggaa
     9901 gaagagaaag ggtcagggta cggtcggcgt atcctccatc gtgagttcgt cgagaaacgg
     9961 catcacgcct ttgacgtacg gcacggtcac tccctcccgt gtcctgagtt ccccgtaggc
    10021 gaggacctcg acgaccagcg ggagctcgtc caggcgccgg atgaagaggg ggtccgactc
    10081 ggtggcgtac cggagaaagg catccagcct gatgaacggg cgctccaggt ccgggacgtc
    10141 gctgtactcc tgcaggtctt cccggagcgt ggaagacctt gccagcgatt cttcaaagta
    10201 gaggaagacg tggaagtcat ggcgctttcc catcgcgtcg agctcccgga tatcgatctt
    10261 cgttcccggg gcgatgccga ggcattccag gtccggggga aacgccgtac cctccggtat
    10321 accggtcgga ctctccatac tatggtgaag ggagggatag tagaaaaatg cacctccggg
    10381 gcacctcatt tgcggggcaa atcttatact caggtggccc aagatgggcg taaggtggcc
    10441 tatggaaggg gaggatccgt cggtatcagg aggagagatc gaggacctca gcagggagaa
    10501 gaaacttcac aggtgcctct cgatgctgga gtcgggcgac ctcaacgcgc ggtggcgggc
    10561 cgcggatgcg ctcggggaga tgggcgacca tcgtgcggtg cagccgctga tcggggcgct
    10621 ggacgacgag tacgtcgatg tccggtggaa ggcggcgaaa gcgctcggga ccatcgatgg
    10681 gcgtgaacct gtcctcccgc tgatacggag cctggaggac gacagttcct gggtccggat
    10741 gagcgcagca tgggcactcg ggaatatcgg cgatccccgg gccgttgagc cgctgatcgg
    10801 gatactcgat gaccccaaac cccgggtcag gaggatggcg gtacgggcgc tcgggcggat
    10861 aggcaacccg cgggccagag aaccccttgt gcgcctcctc ggcgatgccg accgcgaaat
    10921 caggtttgcc gcccggcagg cgctcgacga tatcgggacc gagcgggaga tcccgagcat
    10981 ctgactggcc gactccccct gttgccggga aggctccttt atggaatctg ttcagccccg
    11041 tcggcctctc tatggctcga tccggttgta tcctgcatct cacccgttgg cattgtagcc
    11101 gttgtgctgg ccggggacgg ggctatcctc cgaagtgttc cggggaccgg ggccgccggg
    11161 tccgtcccgt atgagtttat aatctaggaa cgacttcatt tactagatta actgttgcaa
    11221 tcaatttata tacaaaattg agcgccccgg gaggatatca gtatggatct atttatgaag
    11281 tgcgccatcg aggaggcgga agagggtctg aaagagggtg gaatacccat cggttcggtg
    11341 ctggtgcgtg gcggccggat catcgggcgg gggcggaacc gccgtgtcca gtgtaacgac
    11401 cccctcctcc acgccgagat cgactgcctg aggaacgccg ggaggatcca gaactacgca
    11461 gagtgtacgc tctactccac gctgatgccc tgctacctct gtgcgggagc ggccgtccag
    11521 ttcgggatcg gcaaggtcgt cgccggggag tcggtaaact tccccggagc gagggagttt
    11581 cttgaatccc acggggtcga ggtgctcgac ctggacctcg atgtctgcaa gacgatgatg
    11641 catgcgttca tcgaggagca tcccgacctc tggtacgagg atatcggggc actgtgatca
    11701 tggctgaaaa cacgttgctt cgctacgccg tcgttgccgt tgtcgccctc ctcatcgggg
    11761 tctgcgtcac ccttgcggtc gctccgcacc ccgccgggcc tgcgcccgga ggcgacgccg
    11821 atcgggacat gctctttcag gcttcgacga tcgatgcgct cctgcagggc acctacgacg
    11881 ggagcataac gttcgatgaa ctttcaacgc acggggatct cgggatcggc tgcggcgacc
    11941 ggctcgacgg agaactgatc ggggtcgacg gtgagtggta cctgatccgg accgacggac
    12001 gggcctaccc ggtcgccgga aacgcgacga caccgttcgc ggccgcgacc ttcttcgatg
    12061 cggacctgac ggtcgcgatc gacgagccga tgaacctgac cgctctcggg cgttacctgg
    12121 aagcggagct cccctcaaag aacctcttct acgcgatccg gatgggcggc accttcccgc
    12181 gggtggtgac ccggagcgtg cccatccagg agaagcccta cccgcggctt gccgacgtga
    12241 cggcaaacca gacggtcttt acgttcgaga acgtcaccgg gaccgccgtc gggttctgga
    12301 cccccgcact ggcagaaggg atcaacgttc ccggctacca ccttcacttc atcacgagcg
    12361 accgctccgc cggcggacac ctgctcgaca tgacggttcc tgagggctgc agcgtgcaga
    12421 tcgacatgac gccgaacttc accatggcac tccctgcggg tgacttcctg acggtcgacc
    12481 tctcgggcga cctctcggac gacctcgagc gggtagagaa gtagggcgtc agcagtcccg
    12541 gccgggagag ggtctctttt ttggctctgt tgaaacccca cttgtttgct ccagatggcc
    12601 gcaatcaggg gtactcaggg gcacggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    12661 acggtccact gcatgaggac atgcgtggtc cggcaccagc ctcatcaggg taagggtgaa
    12721 gatcagaaca gggtttcaac aaagccaatt tttgtacatt cttcctgtgt ggctgctggt
    12781 gacccccttc gcacgttcgt gtgagatcgc atccgcttac tatacaggca agatgaaacc
    12841 gcacggcacg gggcgacccg gccggaaaaa gagaggttat cggacgacga gcagcggcgg
    12901 tctgaccgcg ccgccgatct gctcgagcag ggcgctgatc cgggtctttc cgcttctgcc
    12961 gaaagtgccc atgacgacca gcccgtattc gccggagttc gcctccctga ggacctcgtc
    13021 ccggatccgg ccctccacca tctttgtcga gcaccggatc tgctcccctc tgtagcggga
    13081 gaggatctgg ttttgatcgt cctcgatgct cttacgcata tccttccaga tccgggattc
    13141 atagtccttt aactggtcgc agatgccgct ctgggcgcag aagttgaacg ccatcgcttt
    13201 ggcctccgcg atgtccagca ccgagaagag gactgtctcg gcattcttcc gctttgccag
    13261 ctcaatcgca tgcagagccg ccttctggct ccacttcgaa ccatcgataa gcacgagaat
    13321 cttcatgtta ctcacatcgc aaatttcagc cagagcactc cgaatcccac cgccaccgtc
    13381 acgaagagca cgagcatccc cactttcagg aaatcgataa aggagatgcc gatcccctcg
    13441 cgctcggcga taccgataac gacgacgttt gccgacgccc cgatggcggt gccgttcccg
    13501 ccgaggcatg caccgagggc gagcgaccac cagagcgggt agacgtccat cgtcgccccg
    13561 aggtcgtgga tgagcgggat catcgccgcg gtcaggggga tgttgtccac gat
//
LOCUS       DAJB01000192            3164 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_110200, whole genome shotgun sequence.
ACCESSION   DAJB01000192 DAJB01000000
VERSION     DAJB01000192.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_110200"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          682 a          911 c          991 g          580 t
ORIGIN      
        1 cggatcgcct ttttctccct catacaggct gaacgggcgg caactaaaca aagaaactct
       61 tagcgagtga aagagccatg accacaacca gaatgatacc ggggcgggac cgatgaggcg
      121 caacctctgc gtcctgctcc tgttcgcact gctcgttggt gtcgccagcg cggccgagac
      181 cacaccggcg gaacagatcg cggtcaccag tgtcaccctc gaccctgaaa ccctcatgcg
      241 cggggatacg gggacagtga cggtcgagat acagaacacc ggggatgccg gcgtcgccat
      301 cagcagggcg gaactctact ccagcggggt tgtcgttgta aacgacagga catacgactc
      361 tgtgggcaca cttggcccgg ggaataccat ctcgtttacc ttcaccgtgc gggccgatgc
      421 tgaggatggg atatactatc ctgtcttcta ccttgacctc agggacggcg gaagcgtccg
      481 ctactccatc ccggtgactg ttgagagcac cgggatccgg gtcaacgtga tcgacgctcc
      541 ggagacattc ccggcaaaca gtgaggacac catcgtcctc tcggtcggga acccccgcgg
      601 gagcagtgtg agcggtgtta ccataacccc ggccggggag ggggtcagga caaccgagac
      661 ggccgtcttc ctgggcgctc ttgcaccgga tgaagcgaag accgccacat tcagtgtcgt
      721 ggcgtcacag tcgacagaac tcaccttcga tctctcctac cggaacggga tcaacgagca
      781 ccacacaacc ctgaccatac ccatagagat cggcgaccgt gtggtcgaac cggagatggt
      841 ggtcaacaac atcgaggtta ccgggagcgg cagcacgatc accctgagcg gcgacgttac
      901 caacgccggg ctcaaagacg cctacgccgt caaggtcacc gtcgatgatc cggcgaccgc
      961 ggccgacccc tacccggtct acgttgtcgg cgggcttgag cccgatgact tctcgagttt
     1021 tgaggtgacg tgcaccgtgc cgcagggtgc atcctccgtt ccgctcctcg tccagtacag
     1081 ggatgaggac ggcaaaacct acagcacaac cgtgagcgtc tccctctcgg gtgcgggcca
     1141 ggcgctgcag tctgacacag gtagcgagaa cccgggcagc atgagcgctg cccctgcaag
     1201 aggtggcatg ggtgcggccg ggtcatttgg cagcgggttt gcaaagatcc cggtcaccgg
     1261 gatcgtgctt gtgatcgctg tgatcgccgt cggcaccgtg gcgtggcgga agggatacct
     1321 caggaaggtc catgaccggt tccgcaggta gtcgccccgg ggtgtagaag catgagcagg
     1381 ccggttatcg agttaaacga tctcagaaag gtctacaccc tcccttcggg cgacgtcacg
     1441 gcgctaaacg gggtggacct caggatcgat gagggggagt ttgtcgtgat catggggccg
     1501 tcggggtcgg ggaaatcgac catcctcaac cagatcgggt gtctcgaccg cccgacatcg
     1561 ggcgacctct tccttgctgg aaagaacaca agggagatgg atgaccatga cctgaccaat
     1621 ctccggttga catccatcgg ctacatcttc cagaagttca acctcatccc cctgcttacc
     1681 gcttacgaga acgtcgagta cccctacatc atgaaaaacc gcaaacccga cgataaaggg
     1741 agggtccggc atcttctcac catggtcggg atagatgagg cgctatcggc gcataaacct
     1801 ggagaactct ccggagggca gcagcagcgg gtcgctatcg cccgtgcgct cgtcaacgac
     1861 ccggcgattc tcctctgcga cgagcctacg gggaacctgg actcgcagac gagcatccag
     1921 atcatggaga tcctggcaga cctccacctg agggggcgga cgatcgttat ggtgacgcat
     1981 aacccggaga ccgccgcata cgccgaccga acaatcacga taagggacgg gaggatcgca
     2041 tgataaaaaa agacatcatc ctcacgctcg cagcaaggaa cgtccggctc cactttatgc
     2101 ggtcggtgct tgccgctctt ggcatagtca tcggtgtcgt tgctatcgcc tcgatcggga
     2161 tggtgggggc gaacatgacg ctctcggtca cggaagaact ctccgatatg gcaaacaaac
     2221 tcagcgtcac cccctaccgt ggtggcggcg gtatgatggg aggagggcct ggcggcgggg
     2281 aagacgagga cgatgaggac gtgatcaccg atgacgagtt ccgtgatata gagcgcatcg
     2341 tcgcgaaata cgggaccgct tatgccctga tgtcggagtc cgatcagatc gaggcggggt
     2401 cggagaccgg acgggcaacc atctacgggc tcgacccgga tatcatccac gagacgctgg
     2461 ttgtcgagac gggaaagttt ccagagagca cgtcctcggt ggtcgtgggc ccgacgcttg
     2521 ccgagcggct tgacctgaag gtagggagca agattaagat aggcgaccct gacgaggggc
     2581 caaccgcgac cgtcagggtc gtcggcatcc tggaagagcg ggggatgtca atggatctct
     2641 ccacggatat ggctattgtc ggttcgcaga aactcttcac cgggatctac ggcggtgagg
     2701 gcgagtatga tcaggtcaac atagtggttg atgatatcaa caacgtcgat gctgtcagga
     2761 cagcgatcga agatgaactc aaccgcaagg aggatacggt caccgtccag gacagcagcc
     2821 gcatgatgga gagcgtaacc tcgacactct ccacgatgac aacgttcgtg atggcgatcg
     2881 cggggatctc gctccttgtg gcagcggtct cgatcttcaa cgtcatgatg atgtcggtga
     2941 acgaacgggt gcgcgagatc ggtatcctga gggcgatcgg aacgcagcgg ggcgaggtcc
     3001 tgcggatgtt tgtctacgag gccgggatcc ttggtatcgt cggcgcggtc atcggtgcga
     3061 tcgcaagcct tgtcatcggc tacgtcgtcg tcttcgggat ggtcggcacc gccgattact
     3121 tcttcgcaat ggatagcctc atctacgtcc cgatggcgat ggcc
//
LOCUS       DAJB01000193            5684 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_44653, whole genome shotgun sequence.
ACCESSION   DAJB01000193 DAJB01000000
VERSION     DAJB01000193.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_44653"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1034 a         1871 c         1799 g          980 t
ORIGIN      
        1 ccgatgtgta ccgcctgtgg gagcgagtat accgatccgt cgtgccggcg ccaccacgca
       61 cagacgatcg cctgtgcggc ctgcgggccc cacctcgccc tctttcaggc cgacgggacg
      121 ccgctcgcgg cggcggatcc cgtccgggaa gcggcaaccc tcctggatgc cggttcgatc
      181 gtcgccatcc gggggatcgg ggggttccat atcgcctgca cagaagaggc ggccggtgag
      241 ctgaaacgcc gcctgggccg gacggagcag cctctcgcag tgatggcgac tcccggggag
      301 gtggagcgga tcgcggtcgt atcggacgag gagcggcaga tcctctgctc ccgggaacgg
      361 ccgatcgtgg tgctcgcaaa acgggacccc gcatcccatc ctgtgctctc gggtctccac
      421 accatcgggt gcatgctccc ctacaccggg ctgcaccacc tcctcttcgc gaacctcgcc
      481 cacccgctcc tcgtgatgac gagtgcaaac ctgccgggct acccgatgat caccgatctc
      541 gcggtcgccc tcgagcggct cccgcggcag gtggactaca tcctcaccca tgaccgccgg
      601 atcgtgaacc ggtgcgacga ctccgtcgtc cgggacggct atatcatccg tctctcgcgg
      661 ggccttgccc cgaagcggac ggcgatcgac ctcggtgatg aatgcatcct cggcgtcggc
      721 ccggaactga acgcgaacat ctctatctac cggagcggct tctgcatcac ctccccccat
      781 gtcgggaatg tcaggaaccc cgggactctc gcctacctgc aggagacggt ggagaatatc
      841 ggggggctcg ccggggcccg gtacgatcgg gtcgtccacg acctccaccc gcagttcctc
      901 tcgactcgct acgcgaggga ggtcgccgaa gcggccgggg cggaactcct cgccgtccag
      961 caccaccggg cgcacatagc ggccgcgacc cgggaggagt gcgtcgggat cgccatcgac
     1021 ggcgtgggct acggcgacga cgggacaatc tggggtggcg aggtctttgc cgggcaggtc
     1081 ccccgcctcg accgggtcgc ccacctcgag gtcgtcccga tgccgggcgg cgacctcgcc
     1141 acacgcttcc cggagcggat gctctacggc atcctgccga cgtccggggt ccgggacctg
     1201 ctggcgtcgc ggggctggag cgacatcgag ctcgcggtcc tgacaaggca ggtggagcgg
     1261 ggcttaaacg tcgccgcgac ttcgagcacc ggccgggtcc tcgacgccgc cgcggccctc
     1321 ctcgggatct gccgggagcg gacctacgac ggcgagccgg cgatgaaact cgagtcggcg
     1381 gcatacaccg gaaagccctc ggcctggaac ctcgacttcc tgcgtggcga tgacggctgc
     1441 gaggtcctct cgacccgctc gctcctcgtg gaggcctacc ggcgttcagc cgccggcgag
     1501 cgggcaggaa acgtcgccgc atcgtttcag tataatctgg ccagaggcgt cgccgcgatg
     1561 gccatccggg cggcggagga acgggggatc ccgcgggtgg cgctctcggg cggggtggcc
     1621 tacaaccggg cgatccggga gacgatcgtc ggtgaggttc gtgctgccgg gcttgagttc
     1681 atcatcaacc gggagtaccc gctcggggac gggtgcatca gcttcgggca ggtggtatac
     1741 ggagggagcg tggaggagta gggtgctccg ccggggaata accgggagaa accactgttt
     1801 ttattcacga tcagtgccgg ggggcggcac tttcccggtc cggctgccct taccgggagg
     1861 cagcgtgcgg ggggtgcgga aggaggggag ctcacctggt agggtacctt cttccaaaga
     1921 atatcgcttt cgtagagcaa ttccgtgaaa agagttcgcc agtccggtga ctcctcattc
     1981 tccaggaggt cgtcgggctc cggggcgtct ggttcgaagt gctctgagga cctcacgaac
     2041 cgggagggtg acacccattt tttatccgac ccgggtgcgt gagatatcca ttgagcagta
     2101 tataagagat ctgttttaat tcttaaactc tcgcaggaca tcaggcaaaa taagcaattc
     2161 agacgggaag ttatatgatt tctagtgaag atgttgctat atgcgagata cctctcttca
     2221 ccgcagagtt gcccatttta ttctctgcct cagcgttctt acagtaacgc tcgtatctcc
     2281 ggcagcagcg ctcatggtcg agctctcccc ttcatatctt gcagggcact cggacgcgat
     2341 agcaaccgga acgatcacct ccgtcgagag ccggtggaac gacgccggca ctgagatcga
     2401 gaccgtcgtc tccttctcgg tcgacgagac ccttgcaggc atactcccgg atgaccccgt
     2461 tctggtcgtt ccgggagggt cggtgaacgg gattacatac tgggtagaag acgtcccggt
     2521 cttctccccg ggggagaacg tcggactgtt cctgaaacag cgccccgacg ggtcctacct
     2581 gccggtcggc ctctcacagg gggtcgtccc cctcgccgga gagtccgcag cgaaagccgg
     2641 ggcggcaggc ctgctcacgc ccgaagagtt cggcgagagg gtggacgccg cgatgcaggg
     2701 cgacgccggc atcatgtgga cgacggcgac atcggttccc gcgccggtcg ggatcgcgga
     2761 ggtctccctc agcgccatcg ccccgacgac cgcgtcggca gggaccggga ccgaggtgac
     2821 gataaccggg accgggttcg ggaccaaggc gtccagggag agcttcgggg acgtcgcgtt
     2881 cttcttcact tcaagcagtg acacgtcgta ctggatctat ggtaccgggt acctgccggt
     2941 caccggctgg cagaacacca acccaaacga catcgtctcc tggaccgact cacagatcgt
     3001 ctgcaacgtc ccgaccgggg ttgcctggcg gggatatgcc tactggggat cggcaagcag
     3061 cggaccggtc tgtgtgctcc atgacgacgg ggtgaccata ctcgggccat actcgctcag
     3121 cgttccgttc gggtggagca aaaaacgctg gcaggggacg gtgcccgtcg tctcctacta
     3181 cgtcaacccc tcctcggtct cgggagctct cgccgcggcg cagaacgctg ccgggacgtg
     3241 gacgtctgtt gcgggctccg acttctcctt tctgtatgcc ggaatcacga cctcgacaga
     3301 cgtgagtggc aactccaaaa acgagatcct ctggggcgac atcgccatag acggggtgct
     3361 tgcgcaggca agcacctggt caagcgcggg gcaggtcgcc gaatgcgata tcaagttcaa
     3421 cacccggtac acctggtcga caaaccccgg cgccggagag ttcgatatcg agacggtctg
     3481 tcttcacgaa ctcggccact gggttcaact gctcgacctc tacgggaacg tccagaacta
     3541 cccttccgac acggcgaagg tgatgtacgg ccgggtcggg tcgggcctga cgaagcgcac
     3601 gctcaccgac tccgacgcag ccggggctcg ctggatctac acgggcctct tcccggcccc
     3661 ggcgatcgcc gggatcaccc cgaactccgg gtatgccggc agtgtcgtcc agatcaccga
     3721 ccttgcagga accggcttcc agaacggcgc cgcggtgaaa ctcgccaggt cagggcagcc
     3781 tgatatcgtg gcgacaggcg tctccgtcgt ctcgtcgtca cggatcgcgt gcacgttcga
     3841 cctcgcaggc cgtgcggcgg gctcgtggaa cgtcgtcgtc acgaaccccg acgggcagtc
     3901 cgccacactc ccgcagggct tcactgtcag gagcccgatc gccctcaccc tcaccgccgg
     3961 cgaggatgcc atcatgcgcg gcaacgcctt cgacctgacc ctcagcggcg agagcggccg
     4021 gacatacttc atatatctcc aggcggccgg agacactacg ccggcggcgt atccgtccat
     4081 cgcacccgtg cagcctggca tcagcgacat cgggacatcc ggcccccggt cggtgacgaa
     4141 cgcgaccgcg acgtcacagg caaacgtcac cgttggatcc tccggcaccc gtacggtcag
     4201 gttcgagacg aacacctcaa cgagggcgca ggactacacc atcgaggcga tcgactgggc
     4261 cgacccctcg cgctcggcga gcgtcacggt gtgcgtggag cggggagcag tgaccatcac
     4321 atcggccggc accggcatat actatattgg tgacgaggtc ttcttcggcg gcacaaacac
     4381 cgacagtgca accacatatc tcttcatcac aggactcggc cttgcagcag acggtgcaaa
     4441 acttgatgat acgaccatac cctgcgtaag tggggatacc gtcacgttta cacgggcgga
     4501 ggttaatccg gacgcgacat gggaataccg ctggaatacc agcagttgcg gccttgcacc
     4561 ggagagcggc tcccatgccg gctacacggt ctacgccgtt gcggttcccg ccgccaaaaa
     4621 tgatctggcc gatgccgcgt atggaacaac ctctctcgtg ctgaggggtg cacccgatac
     4681 gaacaaccag atcgccgtcc tgccggcagc ctcaacgttg tcaatcgggc agacacgggc
     4741 gtacacggtg gcgctcgatg ccgcccctgc ggaactcctc tgttataacc tcaccgtgca
     4801 actgaccgat ccggccgtcg gcgagattgt cggcgtccag ttcccggcgt ggtcgattat
     4861 cccggcaaac gacacggtgc ccgccgatgc ggtctggtgc agcgccaggg attccacagg
     4921 agcgtccgga accgccaata tcacgctggt gacggtaaca gtccgtgccg atgcccccgg
     4981 cacgacgaac atcacggttc ttcctggcag gatcgaagat cggcatggcg gcaggtatgc
     5041 ccctgccgtg acccgggcgc agctccacgt gaatgccccc gtcgcaaact tcaccgccga
     5101 cgtcaccgcc ggggcggccc cgctcaccgt ccggttcacc gacaccacga cggagaatcc
     5161 gtcatcctgg ttatgggatt tcggcgacgg caacacctcg accgagcggc accccgtcca
     5221 cacctacacc ctccccggca accacaccgt cgcgctctcg gtggacggca gcctctcgac
     5281 cgccacaaaa cccggctgca tcaaggtcac gccggtcctc ttcggggatg caaacgagga
     5341 cggggcagtc aaccaggccg acaccctcct cgtcctccag gaggtcgtgg ggctccggga
     5401 gaagcttacc ccgggcaccg acgggttccg gaaggcagac gtccacgcaa acggcgtgat
     5461 cgaggtcggc gacgccctct tcatcgccca gtacaacgtc gggctccggg acatctggtt
     5521 cgaagtgctc tgagaacccc gcaaacagga tagaaaattt ctctttttca ggcgggtcac
     5581 tcctcgtagt ctgcgaactc cgggatacct gccagaggag aaccctctcc gagaggaact
     5641 agtgcatctt gtcagtttat tttaggaatt ttggcgtgtg gggg
//
LOCUS       DAJB01000194           28523 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_43091, whole genome shotgun sequence.
ACCESSION   DAJB01000194 DAJB01000000
VERSION     DAJB01000194.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 28523)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28523)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28523
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_43091"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         5814 a         8409 c         8626 g         5674 t
ORIGIN      
        1 ggtcttcggc aggccgtttc tcgcggtgca ccacatcttc gccgccatcg gccttctcct
       61 cctcacgctg catccggtta ccctcgccat aggggcgttg aatcccgcca tattcatccc
      121 ggtcttctcc cctgggggag gttctgggca ctcgcaagcc ggcctgcacc ttatcttctc
      181 tacattgcgt tcgccggcgt gctgctccgg aagtacatcc cgaagtactg gcggtgggtg
      241 cacggcctga tgtatgtcgc gctccttttt gccgtcgtgc acgggaacct gatcggagac
      301 gattttagag acccgatcat atgggtgctc ttcaatacgc tcttcgccct tgtcgtcgca
      361 gcattcgttc tcaagcggtg gcagaagatg cggaaaaaga gggcatccgc tcagcgtggc
      421 ggtgcacggg ggtgagcccg ggtacccgct gggtgttcgc ctcaccgggg tcccaatact
      481 tcgctaaagt tccgaggaag ccccctggag gttaaaccac tgacttcctt tcgcacctga
      541 cggtgctcat gttccggccc ttctggccat cgcactttgc ggcgtcgcgt gagaccgcat
      601 cgttgccggg taccaaaatt aacgaagtac tgagccctgc gaagggatct ttcgcgtgct
      661 cgaacgcccg gatggaacgg cctgacgttc agtcaggtgc cggcgcactc accgaggggg
      721 ctgctgacag ggtgacaatg agggcctgcg caaccttgat gcagagttgt ttaaccatcc
      781 cgtggatgaa gcggccagag ataaacgtat tgtgatttag tctgacatgg attcggtggt
      841 ttcatcgtaa aagcacttac aaccagttga gttgaatcta tccagtacct ttggatgtta
      901 tacaatgcga ttaagttata tgcttaacta agaaagctct acccttaggt tggattgcct
      961 cgctacagtt cgcgaggagt ctaacactca attaacgcaa ggagaagtca gtgatgttca
     1021 ctttaccctc aacagccagg tgaacatcac aacagcccgt aggcagagtg tattggctct
     1081 cttcgtactt aagagtttac ccgttctgcc tcaggttcca gggtgatgag caatgagtat
     1141 gaagtgtctg gagagagcct tttcgcttct catagcggtg ctgatgataa ccacggcgtt
     1201 cattcctgcc gtgggtgctg cagaaccgaa cagagccgca tcgagcgtga acaccgccgg
     1261 agactcgttc tccggtacga cgacgtttac tgcaggtaca tccacaccgg cgtccggcgc
     1321 cgtatcgaac ggtcatggta ctgccaaggc cgagaaggac ccgccgctgt tctcaaggaa
     1381 caactggtcg caacccacgt cctggaccaa tacctctgta gatatcgaaa atgccgggga
     1441 acccggtgtg gatgcctcgc cggcggcagg tgccgcgcgc gtggtcaccg gttccggcag
     1501 gaccaagatc aaggtccatg acccggaggc caggaccatc cggatcaagg atgccggcaa
     1561 gtctgccgcc gccggcggtg atgacgaact cgagatccgg ctggtgaatg ccaccccgga
     1621 tctcggcatt tttaccgaga tctttgagat cactgcccac cgcacgatta ccctcgacgg
     1681 atcggaggac ttcctggcca cgtgggcgct ccacaacggg gacgcgaacg ccacccgggc
     1741 cgagtggttc gtctgggagg agcgatccca cagcatcgag atccccatca tcgaataccg
     1801 ggaagttacc cttgagagcg ttactcccga ggacacaagc cctgtaagtg ttacccttga
     1861 gaacattacc cctgaagacg ttaccctaga gggtgttgtc caggaggacg ttacccctga
     1921 atacgcccca tcagagggtg ttagtctgga gggtgccatt cccgaagccg tcaccgtcag
     1981 ggaaccctac atcgccggca ccgagacggt ggagcagtcc tggccggagt gggtgccgtt
     2041 cgtccctgat ggggaggtgc tcaacgccgg ggagacccgg ctcttcaagg tcgtctacac
     2101 caaacctgcc gagatcggtc agatcgatat caacaccgcc cccgtcttcc ggggtgtcgc
     2161 gtgccccgag atgacctggt ggagcaccgc ctgggcaaac cgggtcccgg tcaccgtcac
     2221 caacccgccg tcgatcgacg ggtatcccca ccgcgaagtc gtctcgtttc tgcctggaat
     2281 gcagagcgac tttgatgatg tccggttcgt cactaatgac ggcatcgtcc tcgactactg
     2341 gaaagagacc tacacggcct cggattcagc cgccatctgg gtcaacctcc ccgccggtgc
     2401 gacctcgatc tggatgtact acgggagttc ggcagccacc gataccggca gccgggacaa
     2461 cgcttacatc ctctacgacg acttcgatgg tccggatctc gacacaaccc tctggacgga
     2521 cacgactccg ggcgcccatt atttctcgaa cggctggatc tatgacgccc gggctcaagg
     2581 tgccggtgcg ttggtctcaa acgagcctgt gatcaccggc ggcgagccgg tgatcgtgga
     2641 gacgcacatc cacgccgata gtctgccgtc caacgccggg tactcctgcg gggcgttccc
     2701 ctggttctcc gacaccccca tcaacggcct gctctggtac ccggaatcgt cgtcctggga
     2761 ccgctatgtc ttcgtgaacg gcgcggcagc ctggcgaccg ggagcaccgc gcggtctctc
     2821 gaccggcggc gactactaca gcacctggat cattactccg gcaagtcaga cgcataccgt
     2881 cagcgggagc acctcattta ccgacgtctt caccggcacc accgggatct ccagccaccg
     2941 gatgcagatc ctcggcggtg acaacgtccc ctacggccgg gtcgtgcgga tggactggat
     3001 ccgggttcgg aaatacgtcg agaccgagcc ggcattcacc tacggcacgc cgcaatcgaa
     3061 ccccgtcgtg acgacgattg ccgtgacccc gctggatgcc gaggtggtgg agggcgagac
     3121 cgtccagttc accgctaccg cctacgacca gaccggtgcg gcgatgaacg gtgtagcgtt
     3181 tacctgggcg agcgggaacg aaacagtcgg gaccgtgact gccgacggtc tcttttcggc
     3241 ccggtgcagc gggacaagcg agatctctgc atccgtcggc ttgatccacg gaaccgccca
     3301 gatgactgtc acccatgtcg cgacagccgg ttcggcctgg aactggtcta ccgacgggtg
     3361 ggccggctgg gtgcataccg catcctggac caatgacctc caacccagct tcgaatacgg
     3421 tcccgtcatg gtcggcgatc acggggagca cggtgcggat gttcatctgt ctgccggtgc
     3481 ggtgatagcg accgtcgaac acgagttcac cgacccttcc ggtgtcgggt gggacagcct
     3541 gacgctcgtt gcgcgcgtgc cgggaagcga tgtcccttac gggcggtgga tgacgatcga
     3601 agtcaacgat gaggttgtct actcggagtc cgggttctcc agttccgatc ctgccaacca
     3661 aattccaaag gaatttcacg ccgacttccc gcaatccggg accgtgcggg tgaagatctc
     3721 ccacgggcag aacccggcct ggaatcctcg attcgccatg gagtactact ccctcgagct
     3781 gcacccacca tcatccacca cctcccccat cacgatcatc tccccccatg acgatacggt
     3841 gggagccggc ggcaacgtca ccgtggccgg gaatgtctcc gatatcaccg tatccagtct
     3901 tacgctcact cacaacgggg tagcgtcgac cgttccggtc ggggacggga acttctcggc
     3961 agtggtgaac ctcacggctg ccaacaccat cgttatcagc ggggtcgatt ctcagggagt
     4021 cccgctttcc gcgacccttc tcctcgacgg ggatatgctg ccggccgcct atgagcaggc
     4081 gatcgggttt gaccccctcg acgccgactc cgactgctca cagtggcccg gggaccagtc
     4141 cgggaacggg gtcatcgacg gctacgagat cttcgccggc gatctcccgg tctttgcgaa
     4201 gtaccggatc ggcgccgatc cgttcgtggt ggataccgac ggcgacggcc tgaccgattc
     4261 gttcgagttg ctgaagcttg gaacgttccc ggaggagccg ggacaggtaa tggcactggg
     4321 ggacggcgat cccgtcatcc ttgctgccgg gacggacgac gatccggacg gggacggcct
     4381 ctcgaacctc gaggagcagg gctacgggac cgatccgctc tcggccgaca ccgataagga
     4441 tgatctctcc gacgcggccg agatctcgat ggggaccgat ccctgcgacg ccgataccga
     4501 cggcgacgat cttgccgatg cggccgaggt tgccctcggg accgaccccc tgaacccgga
     4561 ctcgaacggc aacggggtcc cggacggcgc cgaggactac tggagcgacg agcggttcct
     4621 cgacgacacc ctgaacctga cggtcttcgg ccgggggtat gccgtcgcca acgtcagcgt
     4681 cgcggaggtg aactacaccc acctgatcga cgaggagatc ctggtcagca aggtctatga
     4741 cattgggttc ggcgacgatg tcgagtcagg aacgattgag atcgcatacg accctaccta
     4801 cgtcgagaat gcctcccggc tctcgatcta ccgcttcgac gaggacctcg ggacgttcgt
     4861 caacgtatcc tcgaccgtcg attccgtgaa ctgggtggtg tcctgtgagg cggcggggtc
     4921 ggcgaagtac gccatcatgg actcggcgcg gtgggatgcg ctgtttgaag aggagccact
     4981 ggcaggaatg agggctcccg gcgttgtgaa ctacgtaacg gtgcatgtca cggatgcggt
     5041 gacagggaac ccggtgagca atgcggcggt cacgttcacc tgtctcagtg acggtttcgt
     5101 gtatgcctat gtcaccgaca gcaatggata cacctccact agctactcgg atgcgagcaa
     5161 tatacagtat caaatcacca agactggtta cagaacgttt acgggactcc tcaaagcaga
     5221 tcctgtctac gatcaggaac acattaccat tcatccctct atcgtcccga caaatgcgcc
     5281 ctgcggcaat atcgacgttg acacggaccc ggacggtgct gatatctatg ttgacggcgt
     5341 cctccgggga ggttcgccgc acgtggtagt ggatctccta gaagacgatc ataccgtcga
     5401 ggtgaagatg acgggatatg agagcgcatc cacacctgtt cctgtttacg gcggccagac
     5461 tacgccggta atatttgact taagccccgt tcccgttctg ggcacgggca cgatctgggt
     5521 gtcggccgga ccagggggtg cccaggtgta cctcgacggt gtctataaag gagttacgga
     5581 cgagaacgaa gagctgacca tctctgacgt ccccgtcgga aaccatcggg tggtgatcca
     5641 gaaggacgga tacattccgg tcacacgggt ggtgaccgtc gaactcaatg agacgaccat
     5701 catctccgtg ggcctgaaca acgccgacct tgacgaggac ggcctgctgg atggcttcga
     5761 gaatggttat cgcgacgggt tcggcaggtg gcacacggtc gacccgtatg cgatcgacac
     5821 cgacggggat gggctctcgg acggctttga ggccggggaa tccgtcaccg acgagaatgg
     5881 caccacctac ttcaggccgc ggagcgaccc ggacaaggcg gataccgacg atgacggtct
     5941 cgacgacctg gcggaattcg agtacggcgc cgatcccttc aacccggata ccgacggtga
     6001 cggcctccgc gacggtgccg accccgaccc gctgacgccg caagccagcg gcgatgtcaa
     6061 tctcgccaag atcgggcggg ccatcatcct cggggcaacc ttcggggaga ccgggcttcc
     6121 gggtgggctc tgctacgggc tggtcgggga ggagaccgcc tcctcgccct actacatggt
     6181 cgggtggatc gggttctcct gcctgccggt tgtgggtggg atcgcggata tccgcgacgc
     6241 ggtgcaggcg atcctcaacg gcgatccggt cggcgcggcg atgaatgccg cgggcgcgat
     6301 cccggggccg ggggacggga tcaagatcac gggggcgatc gtctgcttca ccgggaagtt
     6361 cccgtggaag gcgcgggaac tgggggtgtt gctctcgaaa gagatattgc cgctcctccc
     6421 ggatgcgctg aaactcgatg tctggagagt agtattgtcg gcccgcgaga atgccattgt
     6481 cacgaagctg accaatgagg gctattccgt ctcccagatc caggagatgg tcgcctcgtc
     6541 ccggggtctg acgaagtcgt tcctgatgaa gaatataggg gagttcaatg cctaccccgg
     6601 gaatctgcac ccctatgggg cagggatcaa gaagacggtg attggaagca gtcaggtgac
     6661 tccgagttat actgtagtcg gggatacttt gaatgcaaat tatctccgct ttaccgaaga
     6721 agcatggacc tgggagataa atgtgaagtt ccttgactgt atgattgata atagcgatga
     6781 aattatcctc tcggttcatc cgagtggtgt tacgaactat ctctactcaa atttcaaaaa
     6841 agagatcgac tatttggaag gtgctggata tagcatcagt ggcagtccgg gcgctgatgg
     6901 gtattacggg atgacaatat cttgagtggt cttataaaca atagaatcct ttttgagcag
     6961 ggggtaacat gctagacaac aaagatatag gattattgaa acagattcaa acagcagtcc
     7021 agccgttaac ggctgatgaa gagttccaac ttgaagcaat aagttatggt tcttattatg
     7081 gacaagttac agtagtatta gtctcaagag agatggatat agtatttaac ctgagcttaa
     7141 aaggatatcc cataataggt tgtgttttaa ggccccattc cattaatctc tcatttattg
     7201 cagaatattt attttccgcc ttgggaattg acatctcttt caagagatgt aactccataa
     7261 aaggcttgaa cctaactatg tttaacggga ctcctcacaa gcatcaaaaa ataattgact
     7321 gtcttgttga gaaatccgga tgtactatcg gattaaacag actctgcact gaaattcaag
     7381 ggttaatgcc taatattctt cggatctttc atctggatgc tgtgaatgag actgtccgaa
     7441 tgtatgtgga ggagactaaa aagcgaaagt atccgggtag actttttcac gaactagata
     7501 actgtcaaaa cgaaagtgat catggtataa gaagggaaat cgatgaaacc gggggatatt
     7561 atcttaaaat agaagatatc ttagaggcga ttgatccatt gttcggagta tttgtcttcg
     7621 gcagtgagat cacctattct ctcggatctg gcaaaataga tgttttttgt aacacatttc
     7681 gcaacggcaa aggagatctt ctatatcact ccgaagagaa cggcacagtg ggtgcgtatt
     7741 ttgttctgta ccctcatgac ctcataaggg agaatatcaa aaagaaaaaa gaacctatta
     7801 tattttcagg taagaatatc tttcaattgc tgggaattga ttacttctac aaccccaaag
     7861 gtctccttga agaccttaac gagctatcct ggctaatata tcacaatatc tccaggattc
     7921 aagaggcttt ctcggatgga aatgttgaaa gaacctacga cggattagtc tcgatggaag
     7981 gtaaaaagga agaagagatc aagaaaatcg taagccgtta taaatctgtc atataagagt
     8041 tgaacccttt tttcggacga cgattctttt caaaatatta gtctaatggc gaaatatgtt
     8101 tctcttttat ctggtatggt ctactgatat tcacgttctt tacgtcatgc cttgatgtcc
     8161 tgctattacc gacggggacg gcctccgtga tagcgctgat cccgacccgc tgacctgccg
     8221 cgagcgggac gatcaacctc gccaagatcg cgcgcgagat cctgctcggc gccgtcctcg
     8281 gcgagtgggg cgccgacgac cacgacaaca tctactacct gggcggctgg gtcgcgagcg
     8341 gggtcatcgt catcggcgac ctccgggata tcgccgcgac gatctcgcgg ggcaacctgg
     8401 tcgggacggg gctcaacctc gccgcgctga tcccggggta cggggatgcg gcgaaggtcg
     8461 cggccgtggt cgggaagttc gtcctgaagc acccggagct gttgaagccg gcgatggtgc
     8521 tcctgattag cgaaatactg gatctggacg tgcggacagt cgcaggtgcg agccgtgaag
     8581 gcgcgaaggc ggtgtggatg ctggtccctc tcgcgttctt cgcacctgac ggtgttcgag
     8641 ctccggccct tcggcccgtc actcttagaa taggctcgct tccgaataaa ccatgatctg
     8701 gtcctggagg atctcaaacg gcttaatctc ccgggaatgg gcggaacggc gcatcttgac
     8761 gacctcgagg gccagatgca cctcggtgag ccctgcagat cggatatagc ggaggaggac
     8821 caccgtatcc gccaggtact ccacaaggcc gtacctgctc ccctccgggt ttgttgcgtc
     8881 ggtctcgctc gtcagcacga gggtgcaggc ctcgtcccgc atcacctcga tgagcctgaa
     8941 catctcctgc cgccgggtgg actcgtcctc aaagagtccc tcgaagagcg atatcgggtc
     9001 gatgatcacc cttgaggccc cggtgttctc gatgagggcg ggaagctcgc tcttgatgct
     9061 gctgatcgcg aggttgaagt cggtcgggtc cagcttgaga agatagaacc cttcgccgaa
     9121 gacctccata tcccacccct tctgcgctat ggtggcgcgg aggagttcct cccgctcgtc
     9181 gaggctgata tagatcacct tctcgccccg gcggagtccc tcgtacgcga actggagagc
     9241 gaacgtcgtc tttccggtgc cgtaggtgcc gacgaccgcg cagatgctcc cctcaagaag
     9301 ccctcccgag agcatcgcat ccagcccttc gaccccgaac ttcacccggc tcgccgtcag
     9361 accaccaccc ggatgttccg cacctcgaat ccacccgccg ccgagacctt caccgcgaac
     9421 ttcaccagat ccttatcctc gaggcggggc atcacccccc ggaacttctc aaagtgcatg
     9481 acccgctgcc ggcgctgcgc gctgttctcc tcccagcgga agaggagcgt ggcgtcgacg
     9541 cagtcggcga tctcaagttc ccgtgaccgg tccaggatac cggaggtgag caggaggtag
     9601 atcgtggtat tccagcgttt ggacgcccgc tgcaacccgc gcaggaaggc aatgaacgca
     9661 tgccaccggg gtccctccgc aagcggcgga gcgatgtcgg tcagcgagtc gaggacgatc
     9721 aggctgttct tcgggctccc gtcaagcacc cggacgatct cggcaaggat gttcccgttc
     9781 tccttgcgct gcctcttctg caagcgctcg atgacgcttc cctccccgta ccagtccgtg
     9841 gggacgatgc tggcatcgaa gtagagctcc gagagatcat ggaagacgat attctcctcg
     9901 agccgctcgt agaggcccgg gttgaacgag agggccatct ccttcaggat atcctcccgc
     9961 atccgggtga acgtcacgta cgagatcccg tccgggagca taaggttccc gccggtcgtc
    10021 ccggtctgtt tccggaggga gagctgcatg agcgaagtct gcacaaactc gacgttgccg
    10081 gcaccgtgct cgccgaggag cagcaccgcc gaacctggcg ggacgccgcc gtccagtacc
    10141 gggtcgagcg aggcgatgcc cgtcggcatc cgcaatcgga ggcggtcgtg catagatatc
    10201 tactatatgt ggatgacggt taaaacattc tggtcctgtg cgatatcgaa ggaagttgcg
    10261 gccactgaag gcatctcccg ctaaaaaaag cagatttata aggatgccgc tccacatcac
    10321 gtataacctt acgcagcctc ctgtctcgct gccggcccgt ccccgaaccc ggaggttggg
    10381 cggcagggag cggggatgca aaacgacgta ggtcgtggga acatgcgcaa aacggtcggc
    10441 gatccggtga tgaagaagac gtgcagggac acccctccgg cgtgggtgcc gcgatgacgc
    10501 cggacgttcc gcacccggca gcccatgagc cccctgcccc ggagcgctcc gctctccccc
    10561 gcctcctgga tcggatccgc tcctgccgga agggcatcag gatcgcgggc gggtatgacc
    10621 ccgcggcgca tggccccctc gtggaggcgg cggtccctcc ggcctacgag gaactcgacc
    10681 gctactggat cctcgagggc ctcgcggtcg cggtaattgc ccggaacacg cagacgaacc
    10741 aggcagaata tctcctcttc gagccggtgc tctcggagtt cgagtacgaa ctcctggaac
    10801 ggctcttcga tgacctccgc gacgtcctga tcctcgacga ccacgacatg gcctccgacc
    10861 gccggggggt cctctccggc aaggcacagg agttgctcac ggagtacggt ctcatcctcg
    10921 acgatgcctc gaccttcaaa ctccgctact acctcgagcg caacttcctc ggctggtcgc
    10981 gcattgacgc gttgatgaag gatccccgga tcgaggatat ctcctgcgac ggtacaaaaa
    11041 tcccgctctt cctgtaccac cgaaagtacc agaacatcaa gacgaacatc ctgttcgacg
    11101 agccggcctt aaactcgctc gcgatcgcgc tcgcccagcg ctcaggcaag cacgtctcca
    11161 tcggcagccc gctcgtggac gcgacgctcc ccgacggctc acggctgcag ctcaccttcg
    11221 gcagcgaggt caccacccgg ggcacggcgt tcacgatccg gaagttccgc gaagccccgt
    11281 ttacgcccgt agaactgatg gagacgaaca ccttcgacgt cgaccagctc gtctacttct
    11341 ggatggcgat cgagaacaac aaaagcctgc tcttcatcgg cggcacggcg tcgggaaaga
    11401 ccacgtcgct caacgcggtc gccctcttca tcccgccgct cgcaaaggtc atctcgatcg
    11461 aggacacccg ggagatcacg ctctaccacg agaactgggt tgcgaacgtc acgcgcgaga
    11521 cggtatccga ggggctcggc gccaccatcg ggatgttcga cctcctcaag gccgcgatgc
    11581 ggcagcgtcc cgagtacatt ctggtcgggg aggtccgggg aagcgaggcc cagaccctct
    11641 ttcaggcgat gagcaccgga catacgacgt tctccaccat gcacgccggc gtcgtcgacg
    11701 cggcgatcca ccgcctggag aacgaaccgc tcaatgtgcc gagaaacatg ctccaggcgc
    11761 tcgacgtcat atccgtccag gcgctcacgc accgggggcg cgaccgcgtg agaaggtgcc
    11821 gggagatcgt cgagctcgcc gggatcgacc cgctcaccgg caacctcctg gtgaacacgg
    11881 tctatgagta caatcccgtg cccgacacct tctcctactc ggggcggtcc cggatcctcg
    11941 gtgagatcat ggagtaccgc ggctggagcc ggacccggct cgacgaggag ctgcggatcc
    12001 gacgggcggt cctccttgcg atggccgaac agggtatcag ggactacagg gcggtcgccc
    12061 ggatcatcca ggcgttcact atcgaccggg accgggtgct cgcctccctc gacgatctcg
    12121 gcgggttgat ccggtgatgc tgcccggctg cacgcagatt cgccaccttg tccgccggtg
    12181 ggtgaagcgc gatcctgtac gctaccggag tctccgcgaa gaccttgttg cggcgaatat
    12241 gggagtgacc ctcgaccgct acctcctgcg gacgttcctc gtctccgggc tcttcggcac
    12301 cttctgggct attgcagcgt tcctcgcgat gcaccttgcc gttctcccgc aggtgagcgt
    12361 cgggatctac aacatcttct ccattcgccc gccggcgttc gtgccgacag acgccctgca
    12421 gatcgccgtg agcgtgctca tcttcgtcct caccgcctac gcggcttaca tcttcttcct
    12481 gcgctacccg tccctggtga agaagaaccg gacgacccgg atcaacctcc tgctccacca
    12541 cgccgtcgcc tacatgtatg ccatgcgcca gggcggggcg gagatgatgg cggtattccg
    12601 ggcgatcagc ggaagttcgt cggtctacgg cgaagttgcc cacgagttcc ggcgagtcgt
    12661 ccgggacgcc gactacttcg gctatgacgt gatcacggcg ctccggcacc tgcaggagac
    12721 gaccccgtcg gagaaactgc aggagttcgt gcaggacctt gtctccgtgg tcgagagcgg
    12781 gggggacatg ctcgcctttc tcgaaacccg ggtgcgcacc tatcaggagg aggcccggtt
    12841 cgagcagaag accttcctct ccacgcttca gctggtagcc gaggcctacg tgacgctctt
    12901 cgtcgccggc ccgctcttca tcatcatcgt catggtcgtc atgggttttg tgagcagcac
    12961 gtccgtcgtg cagctctcgg tcattatcta cctccttatc ccggtcggct cgctcttctt
    13021 catcctcttc gtcgacgcgg tctcgatcaa aaccgagggg atcgagcggt acaccgggat
    13081 acgatggctc cacgagttcg gcgacgcccg ggtcgaagag cggaccggtg acgaaccact
    13141 cgtccggcag ctcgcgcact acgaccggat ccggaaactt cggtcattcc tgcaccaccc
    13201 cttccaggcg ttcctcatcg agccgaaccg gacgttctat gtggcggtgc cggtcgcgct
    13261 tgcctatgtc ctcctcgctg tcctcgcgac cccggcctac cccgatgttg aggtgctgat
    13321 cgacgtcctc gacgaccacc tgatcgtggc gctgctgatc gtccttctcc ccttcggtat
    13381 ctgttaccag atctggcgga caaaggtgat ggggctcgag gcgggcatcc ccgagttcct
    13441 caaccgcctc tccgggataa accaggtggg cctgacgctc gcgcaggcga tcacgatcct
    13501 cgtgaaggcg gacctcgggg tgctgaccta cgagatccgg aagatcaagc gcgacatcga
    13561 atggggcgcg agcgtccagg atgctctcgt caggttcgag gagcggatac gcacccccac
    13621 catcgcccgg gcggtcaccc tgatcacgac ggcgagccgg atgaccgggg atatcggcgt
    13681 agtcctgaac atagcagccc gggatgcagc gatctcggag accctcaagc gagagaggcg
    13741 ggccgagatg ttcatctaca ccgccatcgt ctacctggtc ttcatcgtct tcatcttcgt
    13801 cgtggccgtc atcgacgccc agttcctttc cgtgctggcc ggggtcgata cgctcgctcc
    13861 cggcagcatg cccggcggga tctcgttcgg gagcacctct atcgcgacgt tcgagcgtct
    13921 cctcttccat gcgtgtctca tccaggcgct cttcacgggc ctgatcgccg gggagatggg
    13981 ggaggggtcg ctccgggccg gggtcaaaca cgcggcaata atggtcttca tcgcgttcgt
    14041 gatcttcacg gtctttctat aggcctgccc ctcggggctg ccgcgatagg gttttatatt
    14101 atctgcctcc cacctctctt ctggtgatgt catatgtgta cagtcggtgg acctgtggat
    14161 atcgagttcg atgatgatcg ggtgaagggc acgaattacc gctgcaagga gtgtggcgag
    14221 cggttcaagg gcgttggaaa gcggcccatg tgtccaggct gccagtcgga agatgtcgaa
    14281 gaagtctgaa agcagctcct ccgggggttc ctctgccggg ccgggtgtga cgaccgtacc
    14341 cctcggcagc gagttcctcc tcatccgggg agaacgctct tttctgctcg tggggaaggc
    14401 cggttcccgg tttcccctct gcatcgagac cccggacgac gagtactgcc tcgcagtcga
    14461 ccccgacgat ctggtcgtcg tctccatgcc ggaaggcggc ccggttgagc aggcacgcat
    14521 gatgctcgag ctggtccgac ggtaccacat cccgctcgtc gtcctcggaa aagaccatcc
    14581 ggggacgaag cggctatcga tggtcgtctc ggtcgccccg gagatcctcc tcgcctgtga
    14641 tatccggcgg ggaacccatc ccgaacagca cctgctttgc tcatcggcgg agttatcggg
    14701 gctctcgctt gccggggtcc ccggaggtgt tgccatcaga cagctgcctt tcggggtggt
    14761 aatggagcat ctggaaacgg aaaattcctc agcggacaaa caacaataaa tatatgctca
    14821 atttcatcaa atattcatac gccgtgagaa ggagggtttg atgaagaccg atgtcctgaa
    14881 aagcatcagg gtaactgaag aagagtatca ggcgatgatc cgtgatgcgc aggctgagag
    14941 gaagaagagc ctctcggatg ccgagctgga ggctgagagc ctggtcctta aggcacagaa
    15001 ggatgccggg gattacagga atcagcgcct tgcagacgca cgagcccagg cgcagaacag
    15061 atatgcagag attgtcaggg agggtgaagc gcgcgcagag gcgttgaaag caacggggaa
    15121 caagaacctt gcagaagctg tagactttat tgttacacga tttaaggagc agctgcatgt
    15181 taaggcctga acggatgcgc cggctgctca tcgcggcccc gaagggtgag atcgacacca
    15241 tcatcagtga actctatcgc cacaacatct accacatcga agatttcgtg gcagagagcg
    15301 aagctcctga agcgctgtcg atcggtcacc cgctttccgg ggcaagcgat gcagcctcgg
    15361 cgctcatcaa ggtcagggca atcgaaaatg cgtgcgggat agacccggac aacgtagaag
    15421 tcagaggaaa actccccgca tcgaaggtca gggcgatgat ccggacggat cttccggtca
    15481 tccagaagga ggtggaagag ctcatcggtt cgcgctcgat actggaggcg cggcagaaag
    15541 agcacgagca gcgtgcgaga gaacttgaac cgtttgctgc gatccctcta gatctggaac
    15601 tctaccgcgg gtacacccgg tttgccgtct ttgccggaca catcgcgcac gacgtcgaga
    15661 tcgacgtccc ccacgagaaa tatttttccg acaaggtggt cggcaacatg atcgtcgtcg
    15721 tggtgcagac cgagcaccgc gagcaggtcg agcggactct tctcgacgcc gggttccagg
    15781 cgatccccgt cccggaagag accggggctc cggttgaccg cctgagggct catgccgacg
    15841 aggtccagcg gatcaacggc gagatcgccg cggtcaacga gaagatcgcg ggaatccggg
    15901 agaggcacac cgacttcctg gtagcatgtg atgaactact cacggctgac gtagagcggg
    15961 cggaagcccc attgcggttt gctaccacag aggagacctt catcaccgaa ggttgggtgc
    16021 ctgacgatca ggcagacggg atccaggaag cactggagag agcgaccaac ggcaggatct
    16081 acatcgagga actggagatc gaggacgaca ccagggttcc ggtggagtac gaaaacccgt
    16141 cgttcgcctc gccgacccag gtgctcattg acctctacgc acggccgcgg tactccgagc
    16201 tcgacccgac gctgatggta gccattgtgt ttcccatctt cttcgggctg atccttggtg
    16261 atgtggggta cggagcaatt ctgctcgccc tgagcctcgg gctccggaag ttcctgaagg
    16321 gtgacgaagg aaggatgctc ctcaaggtgc tcagtatctc aagcatctca agcatcatct
    16381 tcgggattct ctacagcgag ttcttcgggt acgcgcttcc gtgggatgca atgttcttct
    16441 cccgacacct caacatcgga ggggaagtgc atggtcacgg cccgcaggta gtcgaactga
    16501 tgatcctttc gatctggatc ggcatcctgc acataacgtt cgggcgcacc ctcggtatgg
    16561 tcaatgccct gaaattccac cacggaaagc acgcgacaaa ggctgcaatc gccaatgccg
    16621 gctggcttgg gacgatgtgg ggtatcctca tcctgatctg gtcgttcttc cccatgccga
    16681 tgatgcccga cctgacaggc ttccctgtcg ccatcgcggt gggtgtcgtc ctcctggtgg
    16741 cgggcgtcct cgggattgcg caggagaacg cgctcgagat cgtcgaactt ccgacgatca
    16801 tcagtcacgt attgtcctac acccgcctgg tggcggtggg cttatcgtcg gtcgccatcg
    16861 cgatggtggt caactacatc gcgatcggca tgatgataca gccccagctt gaagcgatca
    16921 ccccggtcgg cgtgatcttc atcatctttg gtgttctcgt cctctttatg gggcacgcgc
    16981 tgaacacggc actcggcctc cttggaggcg gtctgcactc acttcgtctc cactatgttg
    17041 agttcttcac caagttctac aaaggtggcg gcatcaagta caacccattt ggtatgaaat
    17101 caaagtttac ggaggagtaa aaaaatggta gattttggca cagcagaact gactcttgaa
    17161 atggtacagg catcgcagtt gggaatgaga gcactcggcg cggctcttgc agtcggtcta
    17221 accggcatcg cgtccggtat tgcggagatg acgatcggct ctgctgctgt cggagcgacg
    17281 gcagagaacc gtgacgtgtt cggtctggtg ctgctgctca cggttattcc cgaaaccatc
    17341 gtcatcttcg gtcttgtggt tgcactgctg cttctgttct gatggagtga accatgggac
    17401 tcgaagcagt cgtcaacgag atccgggaga aagggcgaag agaggccgag gcggtccggg
    17461 cagagacccg tgccgaagtc gaagagatcc tgaaggacgc acaggcccgg gctgctgggt
    17521 tcaagatggc ggctgaagag gaagcggacc gggcggcaat ccacatcacc aaccaggaag
    17581 cctcggcggc aaacctcgtc gtcaagcggc aggtcttaaa cgcccagaag atgctcctcg
    17641 atcaggtcta ttcggcctcg ctcgctgctg tcggtgatct gcctgctggg ttccacgaga
    17701 aggtgctcac agacctgttg aagagagcgg caaaggagat caagaagggt gtcgtgcatg
    17761 caaatgagcg ggatatccct gttgtcgaga agatcctggg cgagacgaaa tccctctcgg
    17821 gctacaccgt gggcgacccg gttgacattc ccggcggcat catcgtcgag agcaacgacg
    17881 gcgagctgca gatcgactac agttacggca cgttcctgga cgagatctgg gaatccgccc
    17941 tgaaggatgc gtcagatatc ctgtttgcgt gaggaggttc atacatggca gggataagta
    18001 gcggattggc aaccaactac atctacgcct gcacccgcat gcgggtacgg cgatcacggc
    18061 tgataccgcg cgaagattat ctccggatgc tgaatatgag cctgcctgag atcacccggt
    18121 tcatcggcga gaccaactac cggtccgaga tcgatgaact cggcacctcc ttctccggga
    18181 tcaacctcgt cgaggaggcc ttgaactgga acctcgccaa ggagtaccag aacatcctgg
    18241 aacttgtgcc gggggatctg aagtatttca ccgccagtta tctgcgccgg tgggatattc
    18301 agaacgtcgt caccatcctg cgcggtaaga tgcagggggt gcagcccggc aagctcaagg
    18361 aagtcctgat accggccggc aggcttgatc gggtcgctct cgaccgtctt ctcaccgaag
    18421 agtcgccgga acgggtcgtc gaggcgctca ggggcgagcg gttctaccag atcctcgaga
    18481 aggagctccc ccgcgcgatg gagatggggt cgtttgccca cctggagaac gaactctaca
    18541 agggctacta cgcacgactc atcgccgacg ccacggaagg catcaagggc gggagtatat
    18601 tcctgaagta catcaagctc gagatcgata cccggaacat ccagaacctc ttccgcctcc
    18661 gggccgggca ggtccgcgag gacgtgcggg aactgatgat ccccggcggc tcgttctcgg
    18721 tggaggaact gcagcggctc tcagggcttg aagaccggga cgagttcatc gacgccttaa
    18781 agaggcaggt gaggacggcc ccgctcttaa acgcactcga ggcaatccgg ggcaagagtg
    18841 ccctccatga gatcgaggtt gcgctgaccc gggtccagct ggaccagatg gagcggatat
    18901 cgaagcggta tccgttctcg atccttcccg tcgtggtcta cctggaggag aagaagtacg
    18961 aggtcgcgaa ccttcgcgcc ctcgcccggg gcaaggaagc cggcctcccc ggtgagcgat
    19021 tacagggcta cctggtgatg tagaatggag atcgcaattg tcggtaccgg tgaattcatc
    19081 ctcggtttca ggctcgcggg cgtcaggaag acctacgcgg ccgagaccga cgagcggctg
    19141 gtcgagcaca tcaaccaggt gctcctggac agggacgtcg gcattctcgt gctgaagggc
    19201 agcgacatgg agcggatccc cctccggctt cgcaccaccc tcgagaactc cgttaagccg
    19261 acggtgatcg ctatcggcgg agaagagggc ggcctgtcga tgagagagag aatcaagaga
    19321 tcggtgggtg ttgatctgtg gaagcaatga agaaagaaaa agagaacggg gctcagggag
    19381 ccctgaaaag gatttcaggg ccggtcgtca ctgctgttgg tctcagcgca cacatgtacg
    19441 acgtggtgaa ggtcggcaat gaggaactga tgggggaggt catcaagatc cagggtgaga
    19501 acaccatcat ccaggtctat gaagataccg ccggcatcag gcccggtgag ccggtcagca
    19561 ataccggcct gtcgctcgca gtggagctcg ggcccggcct gctgaccagt atctacgacg
    19621 ggatccagcg gccgctcgag gtgctcgtga acaagatggg caacttcatc gagcgcggcg
    19681 tctcggcccc cggcctctcc cacgagaaga agtgggaatt tgtacccgtc atgaaggcgg
    19741 gcgacgtcgt caaggccggc gatatcctcg gcacggtcca ggagacgaac atcgtccata
    19801 aggtcatggt cccgccccgg tcaaagggcg gcaagatcaa gaagatctcg ggcggcagtt
    19861 tcacggtgga cgagaccatc tgcctccttg aggacggcac cgagatcgcg atgctccagc
    19921 gctggccggt ccgtgtgccc cgtcccgtga aacagaaact gaacccggac atcccgctga
    19981 tcaccggcca gcggattctg gacggcctct ttcccatcgc caagggcgga acggcggcca
    20041 tccccggacc gttcgggagc ggcaagacgg tcacccagca gcagctcgcg aagtggtccg
    20101 atgccgagat cgtcgtctac atcggctgcg gcgagcgcgg caacgagatg accgaggttc
    20161 tgactgagtt tccggaactc gaggacccca ggaccggccg gccgctgatg gagcggacgg
    20221 tgctgatcgc gaatacctcg aatatgcctg tcgcagcccg tgaagcgtcc gtgtacacgg
    20281 gcatcacgat cgctgagtac ttccgtgaca tgggttacga tgtctccctg atggccgact
    20341 ccacctcccg gtgggctgag gcgatgcgtg agatatcgag ccgtcttgag gagatgcccg
    20401 gcgaagaggg gtatcccgca taccttgcgg cacgcctctc ggagttctac gagcgggcag
    20461 gtcgcgtcat caccttaaac gacgcgagcg gttcggtctc ggtcatcggc gcggtatccc
    20521 cgcctggcgg cgacttctcc gagccggtca cccagaacac cctgcgtatc gtcaaggtct
    20581 tctgggcact ggacgcgaag ctctcccagc gccggcactt cccggccatc aactggctga
    20641 actcctactc gctctacctc gactcgctca acgagtggta cgaccgcgag gtctcgcctg
    20701 agtggacccc tctccggtcc tgggcgatgg gtgtcctcca gaaggaagcc gaactccagg
    20761 agatcgtcca gctggtcggt tccgatgccc tgcccgacga ggagcagatc accatcgagg
    20821 tggcaaggat gatccgtgag atcttcctgc agcagaacgc ctatgacgcg gtggacacct
    20881 actgctcgat gtccaagcag tacgacatga tgaaggcgat caagcactac gccgatctcg
    20941 cccgtactgc ccagatcggg ggagcgaccc cgcagcaggt cgtcaccata aagagcaaga
    21001 acgagcttgt gcagatcaag ttcaccaggg actacgagcc catgctcgcc aagatcctga
    21061 aggatatgga agctgaattt gatgcgatga gggcggcgtg accatgaagg agtacagaac
    21121 gattgcacag attgcagggc ccctggtctt tgtcgagaag acagagccgg tgggctacag
    21181 cgagctggtc aacatcgtga ccgccgacgg cacggtcaag cgcggccagg tcctggatac
    21241 gagcgatgaa atcgtcgtcg tccaggtctt cgagaccacc gcaggcatcg gcagggactc
    21301 gggcatccgc ttcaccggcg agacgatcaa gatgccggtg gggagggata tgctcggtcg
    21361 tatcctctcc ggcggcggca agccgatcga cggcggcccg gagatcgtgc ccgaaaagcg
    21421 gctcgagacg accggcgcgg ccatcaaccc ctacgcccgt gcgccccctg cagacttcat
    21481 ccagacgggc atctcgacga tcgacgggac gaacaccctt gtccgtggtc agaaactccc
    21541 gatcttctcg gcttccggtc tgccccacaa cgatgtggcg ctccagatcg cgcggcaggc
    21601 aaaggtgccg ggatcgacgg aagagttcgc cgtggtcttt gcggccatgg gtatcacccg
    21661 ggaagaggcc aactacttca tggccgactt cgagaagacc ggcgccctcg agcgggcggt
    21721 cgtcttcttg aatcttgcag acgacccggc tgtcgagcgg atcatcacgc cgcgtctcgc
    21781 gctcaccacg gcggagtacc tggcgttcga cctcggctac catgtgctgg tcatcctgac
    21841 ggatatgacc aactactgtg aggcgctccg tcagatcggc gcggcccgtg aagaagtgcc
    21901 cggacggcgc ggctacccgg gatacatgta cacggacctt gcgagtatct acgagcgtgc
    21961 cggtatcatc aagggtgtca agggctcggt gacccagatc ccgatcctga cgatgcctgg
    22021 tgacgatatc acccacccga tcccggacct gaccggttac atcaccgaag gacagatcgt
    22081 ggtcaaccgt gagctgcacc ggaagggcat ctacccgccg atcaacgtgc tgccctcgct
    22141 ctcccgtctg atgaacctcg gtatcgggaa ggggcacacc cgcgaggacc acaagaaggt
    22201 ctcggaccag ctctacgcgg catacgcgga gggtaacgat ctccgtggcc ttgtggccat
    22261 cgtcggcaag gacgcgctct cggagcgcga ccggatgttc ctcgagttcg ccgatctctt
    22321 cgaggaccgg ttcgtcaggc agggcctgta cgaggaccgg tcgatcgagg agacgctcga
    22381 cctcggctgg gacctcctcg cgacgctgcc ggaagagcag ctcgtgcgta tcgaccgcga
    22441 actgatccag aagtatcacc cgaagtaccg gaagaaggcc caggggtaag tgtccatggc
    22501 gctgcgagat atcaagccga cccgttcgga gctgatcaac atcaagcgaa ggatcaagct
    22561 ctcggagcgc ggctataaca tcctcaagat gaagcgcgat gggctgatcc tggagttctt
    22621 caaggtgttg cagcaggcca aagacagccg tggtgcactg ctggagcggt atgcgcgtgc
    22681 gatggagatg atcgctctcg ccgagacggt cgagggtgcg gtcggggtga aggccgccgc
    22741 cttttcgacg gcggacgtcc ccgcaatctc cttaacgagc aagaatatca tgggcgtcgt
    22801 cgtcccgcag atccagtcgt cttcggtccg gaagggcgtc ctcgaccgtg gctacgggat
    22861 gctcggcacc tctgccgtca tcgacgagac cgcggagtcg tttgagaacc tgctcgaggc
    22921 catcatcgag gctgccgaga tcgagacgac tatgaagcgg ctgctcgatg agatcgaggg
    22981 caccaagcgg cgtgtgaacg cgctcgaatt caaggtgatt cccgaactga ccgaggccag
    23041 agactttatc aagatgcggc tcgatgagat ggaacgtgag gagctcttcc gcctgaaaag
    23101 ggttaaggcg cggagcgcct gatcaaggga acaggtgata cccggtggag atcggtacgc
    23161 tcgctgatgc tgggaagttg acgggaacgg tgatgctgcg ggttgatttc aactctccca
    23221 tcaacccctc gtcaaaccag attctggatg acaaacggtt ccgggagcac ctgccgacgg
    23281 tgcaggcact cgaggatgcg cggcttgtca tcctcacgca ccagagcagg cccggcaaga
    23341 aggactttac gacgcttgag gcgcacgcgg cgaaactgga gcggctgctc ggccgcacgg
    23401 tgacctacgt cgaggatatc ttcgggcggt gcgcccggga ggcgatccgg aacgcaaagc
    23461 gcggagacgt cctgatgctc gagaacctcc ggtttgccgc cgaggagaac ctgaccttga
    23521 agccggagga ggcgaagaag accttccttg tccggaggct ggcgtcgatg gccgatttct
    23581 acgtcaacga cgccttcggc acggcgcacc ggtcacagcc gtcgatcgtg gggctgccgc
    23641 tcgcgctccc gtcggtcggc ggtctcctga tggagaagga ggtcgcgaac ctctcccggg
    23701 tctttaccgg agctccacgc cccgtcacct tcgtgctcgg cgggacgaag gtggatgact
    23761 cgatcgcggt cgcagagaac gtcctcgagc gggggacggc cgaccgcgtc atcgtggtag
    23821 gtgtggttgg aaacgtcttc ctgctcgcgg cgggctacga tatcggccgg ccttcggccc
    23881 ggctgatcga cgagctcggc taccggggcg aggtcgatac ggcgaaagcc ctcctggagt
    23941 cctaccgcga ccggctctct ctgccgcact cggtcgccgt ccgtgaggat ggcaaccggg
    24001 tcgagtaccc ggtagatgcc gtccccgggg atgcccaggt gcttgacatc gggagcgagt
    24061 cgatcaacct cctctcggag gagatctcga ggtccgggac ggtcgtggtg aacgggccgg
    24121 caggcctctt tgaggaagaa gcgttcgccg tcggcacctt cgagctctta agggcggcct
    24181 ctgccgtgga gttctcggtt gtaggcggcg ggcactccgg cgcggccatc gagcggctcg
    24241 gcctggaggg cgagttcacg cacatctcca ccggtggggg ggcggctatc gagttcctga
    24301 ccgggaagaa gatgcccgcc atcgaggcac tggagatgtc caaaaagatc ttcgggtgag
    24361 gggcgcgagg ctcctcatct cttcagagtt cttttctccg gcatcgcccg gcgtggctat
    24421 agtctgccgc gaggccgtct ggtgaccggc tgtttaccct cctcttacag agcaagtgcc
    24481 gatggcaggt tacgccgggt atcgccgggg ctcaatcgca atgtctggca ggatttcttg
    24541 agggcaggtc cggaaaaaaa tgaggtgggg tttggcctca cggaatgagg tggggggcca
    24601 cacccgaaga ctccatggtc cggtgtgtgt aatctcgcct tgtggtcgct gaacgctggt
    24661 gcaggggcac tgtatgcccc tgaccgaacc ctcgtatcgc taggtaggta tggtttgatt
    24721 gggtgtggtg gttgctgatg gaattgaatg actgatgatc gctgggtgtg gtctttcccc
    24781 gatccagtat atattgtgcc ggttatataa tattttccta taaatatctc ggtcaataaa
    24841 tttgtctgaa tactaaatag ggtttttatt tctctctttt aatcaaaagg agggatattc
    24901 tcccgggaaa acacgcccca catctttata tacgtcccgg gttgcctggc ggtacccggg
    24961 agaacccccc ggcccccgcg ctgccgcggg atcactcgcg gaggagagcg acccgggagc
    25021 cgaccgggat cccgatgtcc gcgccgatcg ggccgcactt gaccgacatc aggtacgcca
    25081 cgggcgggag gtcgcgctca tcggagagcg actcgtagtt ggccatgccc ttggcgatga
    25141 cgagcgttgc atggtcgaga gcatcggcaa gggggggcgg gatgagttcc cggttcaggc
    25201 cgagttcggc gatgccgtcc gtggtggtgg tgagcaggtc gaccctgtgg tcgagcccga
    25261 gcgccacggc atcctccatc gtcgcgtcgt ttaagatcgg cgcgccccgg acggcaaagg
    25321 tgacgtgcga tccgttcttc ctgaggtggt cggcgaggag ggcgtcaaag acaatctccc
    25381 cgcagttgtc ggcaaggtag accacccggg aggtgagggc ttctatcgcg ccggtatcgt
    25441 caacggtcag tcccgccgca aactcccggc gaaagaactc aacgaagttg tcggtgacag
    25501 tatgggcctc ggaaccgtaa tccagcgtgt tgccgatgac cgacgcaagc acgaggtcat
    25561 ggaacgtcga gaggtcgccg cgcaccctcc ggcagaccgc cgccgcatcc tcgttgttgg
    25621 cctttttcaa cgaccggtaa ggatcggtgt cgccgatcat ccggtaggcg agccgatgta
    25681 cccggctcgc gatgacgggt gcgggcacct ggtcggcgga gatgcgcggg agaagatccc
    25741 ggcacgccct gacggtctcc cctatgcgcg ccgaatcatc ggtacagagt ctgctttcgt
    25801 actcgacacg gctgatcagg caatccgtgc agcccggagt caatctcata ctgcagataa
    25861 ctgggtccgg ggaagagaaa aaatggtctg atggggccac tgcgatttga acgcaggtca
    25921 gaagaccccc agtctcctag gatggccagg ctaccctatg gcccctctct tataatatgt
    25981 gctcggattg cacatatatc ttcgcttggg agggcgggga tcggcccttc tatactcgca
    26041 tgtttttgta ctcctcgacg cttcgtgtca gctgttccag ttcgctgccg gtgatctcca
    26101 ttctgcccgt caatgcattg acctccgtga ggagctgctg gatgcgcacg tacatcacgt
    26161 acataataaa cagcaggcag atgatggtta ccgagagcaa gactatcatg attgtattaa
    26221 tatcagtcat cgtgtttctc tcctgaatag tgcgcgggca aaccgctcga caaacccttc
    26281 ctgtacttcc tggggctctt cctggaccgg gaagcccgcc agcgaggctg caattcttcg
    26341 gaacgctttt gacgattcgg atcctggcga tttgacgaca accggagtcc tcgccgctgc
    26401 tgccctccgt acgtttgcgt cttcgggaat cgtatcgatg atggacacac cgagattctt
    26461 ctcgatcttc ctccgatcct gatccgtctc ctccagggtt gcccggttga ggatagcccc
    26521 gccgacggtg ccgccgatca tctccgtcag gatcttgatc tttaaggcat ccacgagcga
    26581 ggagatctca aggttcacaa ccagcagaac ctcgtctgcc acagtgagcg atacaactga
    26641 atcagtcccg atgccggcag gcgcatcaaa gagcaggaac tcacagcgat cggtgagatc
    26701 gcacattaca tctttgagcc gctcggggtc tgcgctctga aacccccgga gcgacaggcc
    26761 gctcggcacg accttgaggc cgtacggacc ctcgtacatt gcatcctgga tccgcgcctc
    26821 tcctgccagg acctcgtgga gcgtgaccgg cgtctctgcg agccccagca ccagcccgag
    26881 gttggccata cccatgtcca tatcaacgat gcaggtctcc ctgccatatt gagccagcgc
    26941 tgttcccaga tttgcagtga cggtcgtctt ccctgttccg cctttcccgg aggcgatggt
    27001 gtaggccctg atcatcaaaa ccgtctcaag gatagatcgt agttgtcaaa tttaaattta
    27061 tttgtttgtc tctcttgcta gcaagacttg aatgcgtttt ttgaaccagg atatactctt
    27121 tcccgggtaa acccggagca taaacgtctg gaggtgtctg cgcaattcgg ttccgtgaag
    27181 atgccgcaaa gacgttttgg cctgccacgg cagcgtcctc tgcctcaggg acggcaatcg
    27241 tccgctaatc gtggcaaaaa gtgagctaaa ggcaggtctg gagcaatgct gattcgatat
    27301 tgatatatga tgcgacgcag atatctcaat gagatatgcc ggggttaatc agatgagttt
    27361 tcatcccttc gtggaagaga gtgcccggag acgcatcgat gagatccgat ccgtcgtcgg
    27421 tgtcgctgca tgcgccctcg tatcctgtga gggcataatt atgggcaagt acttcccgga
    27481 agggggcctt tcgtcgtctc ttttcgcaac gatgtgtgca acggtcattg catcggcgga
    27541 ggcagcttgt ggcagtgcca acatagagcg gccgtctatg gtcacgatca ccgcggccga
    27601 cgctacaatc ctcattttga gtgctgggca ggccgccctg atcacagcgg taatagataa
    27661 gtctgtagat ctcccaggag tacagagaca gttattagat atagcagtca gaatcagaga
    27721 ggaggcgtga tgtacacaat actggctgtc gatgatagcc ccatgatcgt cgacgttttt
    27781 gttaccatgc tggagcgtgg tggatacaga ccgatcaccg cctttagcgg cgaggagtgt
    27841 ctggaggtcc tgaatgcgac ccccccggat ctggtccttc tggatatcat gatggagccg
    27901 atggatggct gggagacgct cgggaggatc aagaccaacc ctgcgacacg ggatatcccc
    27961 gtcctcatgt tgaccgccaa gcccctcacg cccgaggagg ccaacgaata cggcgcttac
    28021 atcgaggact acatcctcaa acccaccacc caccatcagc tctacgaggc catcgagcac
    28081 gtgctggcac gccggcactc catcgccgct gacatcaagc gggcgcgcga ggccggcgtg
    28141 gacgcccgga ttgtcgatga atacgaacgc ctttccaaaa gtgtcgatat caacaggcgc
    28201 ctcctgaaga tcctggagac cacctacagc atcaaggacg cccggacggg aatgggcgag
    28261 gacatcacga gggccattaa gagcatggcg gcgagcatca ggctccagga agagcgtcta
    28321 aaccaggtcc ggacccagtt tgcagatctc ttcgaggtcc attgatcagc ttcatttccc
    28381 tactttgaag gggccccttc ccggaccggc gcgctcgcgg ttgcgaagaa tagtgaggcg
    28441 gcaggcgggc caaactggtc agggcagcct tccggaactc ctctcttctc caggaccccc
    28501 taattattat ccgcgtgttc ctg
//
LOCUS       DAJB01000195            6608 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_41962, whole genome shotgun sequence.
ACCESSION   DAJB01000195 DAJB01000000
VERSION     DAJB01000195.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_41962"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1450..1466
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1233 a         2096 c         2046 g         1216 t
ORIGIN      
        1 agcgtttgca gttcctccat cgtccgcggg acctcgccgc cgaagtcggc gacgagcttc
       61 ctcgccgtcc cgacgatgta gcgggccttg gcgtggtgga acccgatgct gtggatgaac
      121 ggctccacct cctcgggctc cgcgcgggcc agcgcctccg gcgtcgggta gcgggcgaag
      181 agggcgtcgc ggatggcgtt caccgagcgg tccgtcgtct gcgccgagag gatggtgagg
      241 atcagcgact caaaaggggt atgaaactcg ataaaatggc gcctgtcatc gacgacggga
      301 tactgcagaa gaagacggcg gtacacctcg caggcggctt cgcggttcat actgatgggg
      361 cagggcagat tcgaactgcc gtcaaagcgt cccaaacgct ctaggatgga ccaggctacc
      421 ctactgcccc cgtgtaccac catatattgg agaagcaggc aaaaatagtt gccgatggaa
      481 gaggggttca ggacagcagg gggtttgaag attcttcaca gacgatggtg ccgacgtagg
      541 cgccttccac gaccgcgcgg tagaggttct ccgggtcgcg gccgtcgagc accagcagcg
      601 ggataccgga ccgctcgatg accttcccgg ccacgatgtc gaggaccgta ttggcgcccg
      661 cgtccatcct gctcccggtg atgatatcga ggagttcctg cggcgtgagg tgctcatgcc
      721 tcaccgcgcc ggtatccttc ttcgggtcgg cgctgtagat gccgttgacc gacgtggcgt
      781 tgatgaggag atcggcaccg acgctctcgg cgaggaccgc ggacacggcg tcggtcgtct
      841 gcgcgggagt gatgccgccc atgacgacga tcctgccggt ctcggcgaac tcctttgcct
      901 ccgtgtaatt ctctgcaacg cgcgggtagg ccgcatcccc gagtccggcc accaggaggc
      961 gcgcgttcag ccgggtcacc aggatgccga gttcgtcggc cgtcgcctca ccggcgccga
     1021 ggtcgcgggc aacctggatg tagcggcgtg cctctccgcc gccgccgacg acgataaaaa
     1081 tccggcattt tccggcgatc ctttcgagaa cagagacata tcggcttata ttgttcgatt
     1141 caagcgaagg gaccagaacc gagccgccca gcgatatcac gatcttcttc attccaccct
     1201 actattatcc ctaatatcgc tcatatacct gttgtgaaaa ggctgcactg ccctgtatgc
     1261 aactccacag aggtctgggc aattaccggc ggctataccg gctgtctcta ccgctgcaag
     1321 cgttgcgggt atcgcggagc gctggtcgtc gagtacgatg acgaggagcg cgatgagcgg
     1381 tagcccgcaa taactatcat ggttcccctc acgcatggat catatgcacg aggcgcgact
     1441 ctaccagaan nnnnnnnnnn nnnnnnctct acgccgccac cttcggcaag atcatcgccg
     1501 aagcggtcga ccccatcgag aagaagccgc tctatcactt cctgcccggg acgctctcct
     1561 actcgctcgg gaccatcggg tgcaacttcc actgcgagca ctgccagaac tggcacatct
     1621 cccagcagac gctggagatg gagtcgctcc gggacctctc cccggagcag ggcgtcgaac
     1681 gggcgtttgc aagccactcc gccagcatcg cctggacgta taacgagccc gccatctggc
     1741 acgagtaccc gcttgcgatg gggacgctgg cgcgcgaaaa aggcctcggg accgtctacg
     1801 tcacgaacgg ctacatcacc gaagaggggc tgcgcgagat ctccgggatg ctctcagcct
     1861 tccgcgtcga catcaagtcg ttctccgaca ccttctaccg gaaagtctgc gggggacggc
     1921 tgcagccggt cctcgacgcg acggcgcttg caaaggaact cgggatgcac gtcgagacgg
     1981 tcacgctggt catacccggg cagaacgact cgatggaaga gatggagacg cttatccggt
     2041 gggtgctcga gaacctcggg cccgataccc cgatgcactt cacccggttc caccccgact
     2101 acaagatgct ggaggcgaga ccgacccaga tcgggaagct cgagaagatc tacgagcgtg
     2161 caaaagaact cgggatcaac tacccgtatc tcggcaacgt cggaggccac ccctacgaga
     2221 acacctactg cccgacctgc ggcagccccg tcatcgagcg gtcggggtat gcggtccgga
     2281 tcgtggggct caaagaccat acctgcacgg catgcggcgg gcgtatcgag tatgtctcga
     2341 agatccagtg aacgccggag gttcctggcc gacaggatgc tcgggacgct cacccgctac
     2401 ctccggttca tgggctacga cacgatgagt gcaaacagcc tcaccccggg cagcacccgg
     2461 gaagatacgc tcctcctcga gatcgcttcc cgcgacgacc ggctcctcct gacccgggac
     2521 cgcgaactcg cacggcgggg cggggcgcag gcggtctaca tcgattccga agaggtcatg
     2581 gaccagatcc ggcaggtcgc cgacctcggc ctcatcgagc ccacgctccg gatgagccgc
     2641 tgttcgctct gcaacacacg tctccggccg gccaccgtac gggagatccg ggaggcacgc
     2701 tacgccccgc ggtcggcccg cgggaaggag ttctcgtggt gccccgcgtg ccggaaactc
     2761 tactggatgg gttcgcacag caccggcctt gagaagcgtt tgagggagat gcgctcctcc
     2821 ccctgaccgg gcacctgctg caggggcccg taaaaacccc aattcagaca gttttgaaga
     2881 attcgggaaa gatatatcac ctctcaggca gtatgaggcg cagcgactgc aggccagtcg
     2941 gtccgatcgt ttatttaccg ggccgcctgc ggaagagtcg gctttgccgg cgcgtgaaaa
     3001 aacgaccgaa agcacattta atggggcttc gtgcacggat cggcgaggcc ctgactgttt
     3061 atggtgaggg atagtttgga acatcttaag atagcatttt tctgctggga gtcgttgtat
     3121 gccgagcgtg tcggcgggct cgcaaacgcg gcgacaaacc tcgccgagac gctggcacgg
     3181 caccacgagg tgcatttttt tacccgggga gagggaggag acgctgagat taacggggtc
     3241 cggtaccact actgccggcc ggcgggggga gagaacatcg tccggtactg ctccgatatg
     3301 agcagaggga tgatcgaccg ctttgccgag ttcgatacac cgtcatttga cctcctgcac
     3361 ttccacgact ggcatgtggt cgacgtgctc cgccggctcc aggagcggga gaccatcttc
     3421 acctaccact cgacggagtt cggcaggaac ggaaaccgtt tcagcggcgg atggccgttt
     3481 tccgagatat cggcgattga acgctacggc gcatcgattg caaaacacat aacggcggtc
     3541 tcctcgaccc tccggcggga ggccatgaac ctctacgaca tcccggactg gaagatcgat
     3601 gtcgtgcata acggcatcgt gcccggccac taccaggcag acgtcgatcc tggggcggtt
     3661 aaacggcact acggctttga ccccgactcc ccgctgatac tctttgtcgg caggcttgca
     3721 tggcagaagg gccccgacat gctggtggat gcagcccccc atcttctgcg ggagcacgcg
     3781 gacggccagt tcgccttcat cggcgacggg cagatgcgtc acggcctcga gacccgggcc
     3841 cggtcgctcc ccgtccgttt cctcgggcga ctggccgatg cagagtacgt ggagctcctc
     3901 aacgcaagcg atatcgtcgc catcccgagc agaaacgagc cgttcgggct tgtgctcctc
     3961 gaggcgtgga gcgccgggcg gtgcgtcgtc gcttccgatg tcggggggct ctccgagaac
     4021 atcgagcacg ggaccgacgg agtcaaggtg cggccgcacc cggactcgat cggcgaagga
     4081 ctctcccggg tggtggattc ccccgagaag gcgctctcca tgggccggga ggggctcgaa
     4141 aaagtgaaga aagagttccc gtggagccgc gttgcagagc ggatggagga gtcgtaccgg
     4201 aaactcgcga agcacgacac catcccctgc tgaccgcccg ctcacggcag catgacggct
     4261 ataaggtgct ccatcaggcg gaacgggtcc gatgagacaa cgggcgtacc cgcgtagagt
     4321 tccatccctc cgatatccga agtccgggaa tggaggtcgc cccggtccac cacccacgcg
     4381 acgtagtggc ccggttcgtc gagcggcggc atatacaccg ccgcgttata actctgcacc
     4441 ccgatgttcc ggtagcactc gagcactttt gcgagtgcag gggcgagatc gtgcggcgac
     4501 tccgtgatga tcgcgacttc gtgctccttt ctcggggtga ggtgcgccat cacccgggca
     4561 tttttgtaga cgagcccgag accgacggcg gtatggacgg aaaagaggtc gtcgtagtat
     4621 tcgctcccgt aacggcaccg gtactcttcc cgggtcctct caatccgtgc cggagtggta
     4681 tatggacggt gggtgagcag cacctgggca tggttgtgca cgacgctcgc ccccgcgcgc
     4741 cagaggaagt tccagatgag gaaggggtag accgcacggg ggtctctgcg gtgcgcatcc
     4801 tggcaccagc ggatggccac gctgatcata tcggcgatct gcggctccgt ggtgatgtaa
     4861 gggttgctct ccttcgcgat gatgatggcg tggaggtagt cgtacttggc gatgttgctc
     4921 gccgtgatcc agtggtcgcc gtcgatcctc ccgaacgtgt cggccggcgt cttctctaag
     4981 gggtttgaaa acggcccttc tttcgccagt gcctgcaggt cccgtgtctc ctcggcgcgg
     5041 ggcgtcagca ccgggcggcg tgcccggacc gaattgaaga gcgtgctctc gcccgtatgg
     5101 ttatttgtca ccctgacgat ctgctgcgtc tcgatatcgc cgtacttctc ccgcacccac
     5161 tcccgcattg cgtcggggaa gatcaggtgt ccgggttcta cataccagga gtagatccgg
     5221 gagacgagtt cttccatatc cggcgcgagt tccttcacaa tgcccggcag cctggtgata
     5281 tcaagcttta ctgatggcat agccttggga ttaggaggat tataccattt aatccttggc
     5341 taactggatg tgagagatag aggtatgcct tcgcccgggg agtgagcgtc ccccggccga
     5401 tggtcggtca tcgttggtcg ttgtcgcact tttcaccgcc caaatctgcc tcagattgac
     5461 ggggtgcccg cagaacggcc gtttccgttt cagttcccgg acgcgcacac acgcacgccc
     5521 gtgctgccgg gggcctcctg cccgggagaa cagaatcgtc ccagagccac acagacggcc
     5581 tccagcggcc tcacccccca ggacggcaaa atcatacagg gtgtggtcat tgagcccgcc
     5641 ggtgttgcac tgtggcggcg tcccggtgca gcgggcgtac ttgatcggga aattgtaccg
     5701 ggagacgaag aaggagcagg gcggcgaccg aggcaatcag tacaccgcgc caaaaggaaa
     5761 taatttccct ttgccaacca ccgctgagcg catcgccgag cagttcaacg tcggacacgc
     5821 taccgttcgc cgcgccggac cgactgcctc gatgtggccc catcccaaaa ccctcgcgca
     5881 cgcgccgtac cctgctcggc gttgccgacg cattgtttcc accaccatcc cataaccctc
     5941 gcgcacgcgc acacgccgga acactgtttc gctatgttcc gcaagggcgt ccataaccct
     6001 cgcgcacgcg cacacgcctc accggaagat cctgcccagt ccccggccgg tgatcttccc
     6061 ggccactctc cgggcgatcc gtctgccgac ggtgcccgtc cggacggcgt tgacgtcgcc
     6121 catgatccgg gccgttttat agagatgtcc ccgtatgccc gtcactgcct tcgcctcgga
     6181 tccgcaggtc cgtgctgcag gtcgtcgttc gccgcgtccc ccgggccggg ggtgatctgg
     6241 atcccccggg tatccacgtc caggttgtgg aggaccgtcc gcacgcccgc gtgattcacc
     6301 tgcatgggga tcccctgttt ccgggcgtaa tcgatagccg cagatacccg cgcagcgacc
     6361 tcgtgcccgg tgaggtcccg ggtgaaaacc gtatcccgat accccccctc gatcggggtg
     6421 gagccgtcgc gctcgacgat ataaaacgcc ggccggaaat taccggcccc gtccactgct
     6481 gcccgctggt ccgtgtgcga aaccagaatg ttccgacgca tcaggccgcc tctggctctt
     6541 tcccgaaggc ctccgcgtac gtcaacccat ggtcgggcag gcgctcgtag tactgccggg
     6601 cctcgtcc
//
LOCUS       DAJB01000196           12799 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_107197, whole genome shotgun sequence.
ACCESSION   DAJB01000196 DAJB01000000
VERSION     DAJB01000196.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12799
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_107197"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    4539..4563
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2270 a         4162 c         3888 g         2452 t
ORIGIN      
        1 actccttgct acatctcttt gaagatccgg gtcaccggcc gcgccggaga gagcgtcggc
       61 tttaccaccg ctgcgatcga gagtttcggg aaggcatttc tcctgcttcc cgactgcctt
      121 atcggatggc tcgccatgcc gggatcaaaa cagcggctct ttaaccggat ctcgggaacc
      181 gtcgtgatag agacccggga gagggaagag ccggaaggcg tcacctacgt gaagagcaag
      241 gagtgagggt cagtccccgg caaccgggag cccggcagcc tgccaggcgc tcatcccacc
      301 ctcaatctcg tagacttcgc ggaacccggc ctcccgcatc aactcgcgga cgccggcgct
      361 ccgtccgccc cgctggcagt atatgatgta ggttccgtcc gggtcgaggt cctgaagatg
      421 ctccgagaac tctgccgagt tgatgttgac tgcacccggg atatggccgc cggcgaactc
      481 atccggcctt ctgacatcga cgacgacaaa acccggatct ccttccctct cttcgatgag
      541 ggcggacgcc ccggcaggcg ggatggtccg gaagatcgag tcatccggtt ccggatcact
      601 gccgaggcat ccgccgacga gcacggccac aagggtgctc ccggcgacga gcaggaagac
      661 gagcgggaga tgagggtcgg tcatacacct atgtcatgac cgcagagata aaagggattt
      721 tggtcctccc gaccgcttct gtcacccatc ttcgccggcc ggtgcgggag tggagcactt
      781 taagaggtat cgtcgaattc gagcggggta tcgtcgagag ccatcaccgc acggagggta
      841 tccggcgtat ccagggctac gacctccctg ccatccatca cggcaacccg ggtggagatc
      901 gcctctgctt catccatatc atgcgttgta aagacgatcg tcaccctccg ctcacggttc
      961 agccgccgga gcaggtccca ggtctcccgg cgtccgggct cgtccagccc cttcgtcggt
     1021 tcggcaagga agaggacatc cgggtgagta agattagccc gggcgatctc aaggcgctgc
     1081 accatcgcgg gggagtaggt ctcaaccgca gcgtcggcgc tccctgcgag gccaacgaat
     1141 cccatgacct cggggatcct ccgtctccgg acctcgtcac ccaggccgtg cagtcgcgcg
     1201 tggaggtcca ggttctccct gccggtcagc gcagggtcga gcaccggctc ggggaggacg
     1261 atacccatgc tccgccgcac gtccccgagg ttcccgagga tcgcaagcgg ggaacgctcg
     1321 gcaaacccgg agaccgggaa gatcagcgtc atgagaatgg cgagcagagt ccgccggtcc
     1381 tggccacagg ggcagagaat accaaagacc tctccgctct tcacgtcaaa cgggatcggc
     1441 ccccgtgcct ggagccgctc caggtgccgg atcaggttct caacagtaaa ggcggtcatg
     1501 ttgctccgca cagatgcaat cagtcggata gaacagagtc gttgtcaatc acccactacc
     1561 ccgacacgta catccgggaa tgccggatgg cgacccacct gcgggatatc ccccggggat
     1621 gtgctatccg gtttccgtta tccaattcga gggaggaact attgttttag tatcattttt
     1681 ggttagccct atatgaatat atctatttat aaatgaatat aaaataggcc atttaacttt
     1741 tctctattct attgaacccc ccttttaccg tatggctggt gagacgcatc tttacgcgag
     1801 agagcaaccc cggctggccg ggcaaggccg gccgatattg tcccggcccg gaagccctcc
     1861 ggtcgaagcc gcgggaacgc cctccacatc ggccggggag acccaccggg gcgatactgc
     1921 tgaaactatt tatggcatgc acccatccgg tatacccgcc ctatggagac cgaactgtcc
     1981 acgatcgcga ttatgccggt tcccttcagt ccggagcaga tcctcatcgc gttagccgtc
     2041 ctcataccgg cactggtcat attcaacctg ctcctcatca ccgagggggt ctttgaatcg
     2101 atcgggctcc gattctacca ggcggtgctg gtgaccaccg gagcgctgct cgggagcctg
     2161 atcgacatcc cgctggtccc ggtgggcgag gcagtcatcg cggtcaacgt tggcggcgcc
     2221 gtcatccccc tcttcgtgac catcgggatg gtcggacggg gccgggtctc tcgcccgaag
     2281 atcctcgctg ccgtcggcgt cgtatccctc gtcgcctaca tcttcgcaac gccggtcccc
     2341 ggcatcggga tcaccatgcc gttctacatc gcgcctctcg ccggggcagc cgcaggactc
     2401 ctcctcgccg gcggctgcca caccgctccc gggctcgctt acgcgggcgg gaccatgggc
     2461 accctgctcg gcgccgatat cctcaacctc gcgaaccccg acgtgctcgc ggcacttgcc
     2521 ggaggccagg cgactgtcct ctccatcggg ggggcgggca tattcgacgg catcttcatt
     2581 accggcgtca tctcggtcct gcttgcggcg tacgcaggaa gaggcctgcg gaagaagggg
     2641 ggagcctgtt cgcaggagga aggggagtga tgatgcgaag tgcgacggtt cgtgagtgcg
     2701 actgccggga cggcaggagc nnaaggtgcg aagacgcgaa ggagactgcc ggtaactatc
     2761 cggacttcgc gttcttcgcg gcttcgcgtg aggcaccgtg tcacacgtca aaccgtcgca
     2821 aactgcttca accacatctc cgcacaggct atccgccaga actcggtgga gtacgcgctc
     2881 ttcccctcaa ggaactcccg gtagttccgg agcacctgct ccgcgtccca gtagggcctc
     2941 cgggagaatt cgggcgagga gaagagcgcg aggatatggg atgcaagttc atccttcatc
     3001 cagacctctt cgggggtgac gaaccccatc ttgtccatcc tgcaccggat ggcgtcgggg
     3061 accagcccgc gaatcgcccg ccgcaggacg tatttcgtga cgccgtggcg gaccttctgg
     3121 tcgagcggga gccctgcgag gtactccacc agccggtagt cgaggaacgg gacccgggcc
     3181 tcgatcgaga aggccatcga gttccggtcc tcccagtgca gcaggagagg gaggttcgag
     3241 gcgacgacct cccgtttcag cacctcgtca agcgatcccg cgtaccggag gacctccggg
     3301 ggagagccgc ggagaagccc cctccgctcc gaccggaccc ggaactggcg aaatgcccac
     3361 gagaagaagg acccatgatg ccgcgcgctc ccgatcccct cccggagggc cgcgaggatc
     3421 tcccggcgcc ggaggaggcc gcggatgtag ggcgcctggt aggcgatgta ccccccgagc
     3481 tgctcgtcgg cgccctgccc gtccaggacc accttcacct cgctttgggc gagccgcatc
     3541 acgcagtact gggcgtagat cgagagagac gcaaacggct cgtcctgcag gtaaaggagc
     3601 cgtccgatat cctcccagag tccctccgta tcgggagtgg tccggcggct ggcgaccttt
     3661 gtcgccgcaa ccaccgtgtc gatatgcctg ctctcgtcga accgggggtc gtcgaaacag
     3721 accgagaacg tcttctgccg gtcgccgacg ctctccgggc tctccgcccg gaggagaacg
     3781 ttgatcaggg cggtgaccgt cgaggagtcg atgccgcccg agaggcaggt gccgaccggg
     3841 acgtcgctcc gaagcctgag ccggaccgca tcggtcagga ggtcgcggac ccgccgggcg
     3901 gcggtctcgt cgtcgatggc cgcacgtgga gcgccgttca tcgtgacgtc ccagtaccgc
     3961 tccggctccc cgacgctccc ctccgagacg acgagtgcgt gggccggcgg gagctggaag
     4021 atgccgtcat acatcgtctc ggcggtatgg tccgcgaccc cccacgcaag aaacgtcgag
     4081 agcatacggt cgttcggcct cccgccggcc agaggatgtg caaggagcgc cttgatctcc
     4141 gaggcaaaga ggaacgaccc tccggccacg gtatagtaga acggcttgac gcccaggcgg
     4201 tcccgggcgc agaagagttc gcggcggcgc tcgtcccaga gggcgaacgc ccacatcccg
     4261 ttgaaccggg cgaggcagtc cctcccccac tcctcgtagg cgtgcaggat cacctccgta
     4321 tcggttgccg aggcgaagcg atggccggcg gcgaggagct cctcccggag ctcgcggtag
     4381 ttgtagatct cgccgttgaa gacgatccag agcgatccgt cctcgtttgc catcggctgc
     4441 cggccttcgt cggagaggtc gatgatcgcg agacggcggt gggcaaggcc gaccgggccg
     4501 gagaagtggc ttccctcgcc gtcagggcca cgatgagcnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnaggcccg tgccgttgac gtcgcaggag gggcagggac ccgaaccgtt caacacccgg
     4621 taggcgtccg cgaggaccgc ggagttcgcg agcatccccg cgatcaggat cacttccagg
     4681 atctcggccc gggttgcgcc cttcgccatc gccgactgca tgtgatactc gacgcagtgg
     4741 ctgcacttga gcgcggcgcc caccgcgagg ctgatcagtt cgatcacctt cggctcgagc
     4801 tggctggtct cggtaacggc gcccttgtac aacaggtgcg agatgagaat ctcgggtcgt
     4861 tccgccatcc gctcgaatat cagggggacc ctgccgtatt cggcctcgat ctcccggagg
     4921 agttcccccg cgatgacgtc cgacccccgc tgtgcaaccc tcgtgagtat cttctcaaaa
     4981 tccatcgtta caccaagatt ctggtctcag agaagggttt aatccggatc aatatgtagt
     5041 tgcgcatccg tgacggcgta acgctggcaa tatccccgat ggtgaccccg ctggcgacct
     5101 caagcgaccg tacggcatcg gcatacaggt cataggggag tttcacccgg aggccttcca
     5161 tctgggcggt caccggcgtc gggtgcgtca gcatgcttat ctcgtggatc cgctcttcga
     5221 ggatcgccat caggcggggc gggaagatag agagcgggtc ccttgcgagg tcgtcccgcc
     5281 gggcgtccag ggtgcgggag acctcgtcca ccagttcgtc gagtttcgcg agttcctcgg
     5341 ggcgcttgaa ccggtgcatg atcctgccga cgcctccgac atacccctcg acgggcgggt
     5401 cgatgatccg cagcagggtc tcgcggtccg gggcgccggt caccacgatc gggacgtgga
     5461 tcccgcgccg gagcaccggg aacttctgcc ggatacactc ctcgaagttg ccgagagcgt
     5521 agacagcaag atcgtgctcg ttgatgacgt cccgctcctc atcgttgagg tttgctatcc
     5581 gcttcccaaa ccccctcgaa agtccgacca tgttggtctt cgcgcctgag cgccggaggt
     5641 actcggcgat atcgcaggcg gtatgcggga ggtgatggat ctccaggctc gggctgacca
     5701 cggcgatctc ggtcccgacc aggggcgcct cgaccacctc gcccgcgagc ggttttgcga
     5761 tctgccgaat caggtcgata tcgtcccgcg gcacgaacga ctggagcacg acgtcctgag
     5821 ccatgacatg tttctggacg atgtagcccc cgaggtcgtc gatcaggtcc atgatctcgt
     5881 cgtgccggta gacgcctccc ttgtaggtca ccggcaccag cgtcgtcaca gcgtcacccc
     5941 catctttgca aagatcggca tgaccatcgt ctccacgatg tcggccggca gggtatcctc
     6001 gcttgccaca tagtaaaact tctcatcccg gatatactgg cggcggacct tgaacccctc
     6061 gggtgcgatg tactggagcg cgtagatgac gtccatatag agcgtctcgc tcggatcggc
     6121 gaccaccata tgttcgagtt cctcttcccc ggcaaccccc gccgggagga cgatcgtgaa
     6181 tcggtcgggc tggtcgacgt tctccttgcc gtagcgggcc cagaggttct ggagcagcgg
     6241 cgcaaggtag gtctcgtcgc cgatatcaag cacgatctcc ttctcaccgg ggttgacgtt
     6301 tgcaaagtcg ctgatacgga tagcacgcgg catatgacgc agcactcctg cggcgacgaa
     6361 gatcggcacc ttcgggtcga cgtagatgtg cagccggtcg accaccttga tcaggtcgag
     6421 atcctggagg acatcggcca cgatccgttc gtaagccttt ctcccgacct catcgggaga
     6481 ctcgaccacg aagtagttca aggggggcat ctactctccc tggacgaacc cggacgcaag
     6541 cagggcagac ccgaccgcac cgatgtactg cgagtagggc gggacgacga tctcggtctg
     6601 caggagctgg cccatcgcat agaccagccc ctcgatcagc gaggtgccgc cgaccatgat
     6661 caccggctcc ttgatgtcga tctcctgcaa ctgctgctca aagacctgtt ccgcgacgct
     6721 gtggcaggct gcagccgcca cgtcctcacg cgagctccct gcagcgagcg cgttcaccag
     6781 gctctgcgtc ccaaagacga tacagtaact gttcatcgag acgttcttcc ccatgccctt
     6841 catggcaagg ggcccgagct cggtgatatc cacgccgagc cgtttcgcgg tcatctccag
     6901 gaaccggccg ctcgcgcctg cacagatccc gcccatcgtg aaggttccgg ggataccgtc
     6961 gatgacgctg atagccttgt tgtccatgcc gccgatgtcg atgacggtcg cggagccctg
     7021 ctggtggccg gcgaggtaga cggcgccctt cgagttcacc gtcagttcct cctggataag
     7081 gtcggctccg aggtgctttc ctatcaggaa ccggccgtag ccggtggtgc cgatcgcctg
     7141 gatatcctca cgggtaagcc ctgccatcgc aagcgcgtct cgaaccgcgc tctcggcgct
     7201 cttgatcacc tcggtagtcg ggtgccatcc ggtgccgacg atctgattgt ccttcatcac
     7261 gaccgcctta gtcgtgctcg accccgagtc gacgcccatc gtgatcccgg tctgctcctc
     7321 gcgggcaagg agcgctcttc tgcgtgcgat ggtggtcagg gcctccatcc tcgtgagaag
     7381 agttccggag gtcgtccgct cggtgaagga gtaacttacc accggcagct tcgagtgttc
     7441 gggaatatac cgcctgagtt cgttcctgac gatagccgcc tcggcgcagc ggaagcaggt
     7501 gccgatgaag accccgtccg cctccacctt cccttccacc agggccattc cccgggcgat
     7561 catcagtttt aagtcggcgc ttctgacatc cagcccgaac accttgaccg cccgctcgac
     7621 gtcggcaagg gcgatgtcgg ggaagaacat ctccgcaccc accgactcgg cggcatcgtt
     7681 gatctccttc tggacgccgc tgtactccgc gccacaggtg agctgggcaa tccggacctt
     7741 ccggctcacg cttcacctcc gttggggaga ccggcgagga actttttgat ggcggcgacg
     7801 aactgtatgc cctcctcctc gttcgtggga tatttcagtt ccaggatcgg catgcccttc
     7861 tggcgcaggg agaagaggat caactcgtcg gtgcgggcgc atcccatgca cccgaacgca
     7921 aggtcggcgt cggtgacgat caccgcagcc tcggcctcct cgatgagagg cccatagatc
     7981 gccatccgcc cccggacacc ggacggcacc tcgacggccg cgtacttcag gccgcgtttc
     8041 ggctcttcgg gggtgatctg caggggcggg gagtcgaccc cggcggtctg tatccgctcc
     8101 cggataccga gggctgcacc gagcggctta tggccgaacc gggccaccag gtcggagagt
     8161 atgaggctcg ttgcaggata gataaacacc tttgccattc tattcagggc tcctctgtct
     8221 caataatttc ttgtaatcgc ttgatatcaa acacgggttt cttctcgcgt acgggagccg
     8281 cctccggcac ctccgcctcc tctccccgcc ggtcgagttc ctcaaggccg tgggtgatgt
     8341 agcggaggat gcggaactcc cgctcgtggc cgaggtagcc cggcctcgcg ccgccgaggt
     8401 tcgcccggca ccgccgcgga tccccgggcg ggaacccccg atccttgacg aagatgtgat
     8461 tggggtcgaa ggcgcggatc tcttcgatga ggcggtcgac ggactccggt tcgcccgtca
     8521 cctgcagccc gaagcacgtc tccttgatca ggacgccctc ggacgcctcg tatgccctga
     8581 gcgcgagctc gcccggggtc atggacgggg actcgacgaa gacgtacttg gtcaccgtgc
     8641 ccacgtagtc cgggatgtat tcggccatca tttaacctcc ttcacgtata ccgtcgtccc
     8701 ttcgcgcatc ccggcaaggt tccccatatc gagcaccttc ccgatgatgt tcgtgcccgt
     8761 gaacggctcg gaggtcgggc cgaactcggc gttcggtttc gtgcgaacgc cgaccatgcc
     8821 ggtgccccgt cgcgagtcgt tggtcatcgc gagggtgaat gccggcgtct cgcctgtagg
     8881 aacattctcg gggatgatcc cgacattctt ggcgatcttc ggcttgaaga ggaagacatc
     8941 ctcgaacatg aagacaagcg gcatcttccc gacggcatgg tgtttgagcc cggtcgcctc
     9001 acggaggatc gcgaccgacc ggggggcggc cgcctcgtcg agggtgatgc tgatgacgtg
     9061 gtccggggac tctgttgcaa cctcgacggc cttcgcagcc agcacctcga tcgtcgttcc
     9121 cggcatctgg ccgatcacca cacggtcttc cccggctgta tcggcggtga gcgcgatacc
     9181 gcgacgggcg gcgaccgcct ctgcctcggc gagcgaaaga ccgaccagat cgaactgttc
     9241 cggttcggcc cggatcgcaa ggatgtcgcc ctcgccggca aaccggacca gctcgatgcc
     9301 gtggacaacc ttgcctacga cggtgtgcgc cgggcttgcc gatacgccct gcgtatagat
     9361 gtagactgct cccgaggatt tcccggcagt cctgacggta atggtccctt caagcctcgg
     9421 accggagaac tccatcggaa ccttcgtcgg gaccagatgc gtatcggcga tgtaggtgct
     9481 gcccgaacgg tccaccggga atctgccgct ctgcacggag aggagaaagt gctcgacgct
     9541 ccgggcagtc tcggtatcga cctcgcctgc ctcgaagccc agcacttccg cttcgacgta
     9601 ggagatgatc tgcatcccgt cttcgagcgg gaactccgca tcgcgggtga cgatcgcatg
     9661 cgaccggtcg gcacgctgga atatccgctc gacctcgatg acccggtcac cgtcgccgat
     9721 gcggtcgagc agcccccggc cggtgacgac cctgccgata acgccgccgt ctgccggggc
     9781 gccgtagtcg gcggtatgcc gcatgcgtgc gaagatcagg tgggattggg acgggtcgta
     9841 gcctccgcac ccgaggatca catcgccacg ctcgtaccgg cccggttgcc gtgccggccg
     9901 gacgacggac gcaaacggcc caaaggcggc ggcgtagcgg tcctgccagt ggaggcggag
     9961 ctgttcggca agcccgggca aaaagaggcg ggagaccatc cccgggtcgg ccatctcgac
    10021 gaccatatcg cccgcagagg tgaggaacct gacttcctgc gactccgccg catcctcctc
    10081 gagtgccggc cggatgacag cgacgctgca tcgcacgtcc cgctccggga ggagatcccc
    10141 gagccgggaa cccgccggga cttctctacg ttctccgtcc aggtggatct ccatcttttc
    10201 acacctcagg gggcgccggc ccccatcacc tgtcgttctt cctcggtgag gtgagcgagc
    10261 acctcctcgg tcgggcccat ctggatgatc ttgccccccc gcatgagggc gacgcgatcg
    10321 cagatatctc gcacaaactc catatcgtgc gagaccacaa taaacgtctc gtccatctct
    10381 tcgcgggcat ggaggatcga gtgtttcacg tcgaccttcg tgatcgggtc catcgttccg
    10441 gtcggttcat cgagaacgac cagcaggggc tcgcgaatga ggacctgagc cagcgccacc
    10501 cggtgcttct cgccttcaga gagctgaccc ggatagcgct ccaggatctc ccggctcttc
    10561 tcgggcgtaa acccggccat cccgagggtg atgatcgcct tcctcatcgc aagttccttg
    10621 gggaactcaa ggccgatggc atcggtgagg ttgtcgagca ccgtcctgtg cgggtagaga
    10681 tcgtactcct ggtggagaag gcctatgtag ccctttgccc ggccacggtt ttcgatgccg
    10741 ggtttcgtca tatcgatcca gtcgtccccg atccggatat tcatctcgcc gctggtgggc
    10801 tccaggatac cggatatgat ccgggagagg gtcgtcttcc ccgcaccgct cttgccgatg
    10861 atcccgaaga tctccttctc gctgacatca tacgagacac cgttgaccgc cctgacgacg
    10921 ccccggtcga ccgagaggta acgcttgacc acgtcccggg cgatcaggat cttctcgccg
    10981 acctccccgg cctcgtgctg ctcgatatcg ctgtagttct ccatgaactt gcgggtgact
    11041 tcctccgggg ccccgatcac cttgatctcc ccgtcttcaa ggaggattgc cctatccacg
    11101 acatcctcga tgacgtttga gaagtgcgac gtgatcacca tcgccatatc gttttgctgc
    11161 gcgctctcga tgagcatccg atgaacaagc gtcgcggtct ccgggtcgag cgtcccggtc
    11221 ggctcgtccg caaagagcat gaacgggttc ttcgcgagct gtcgggcgag caccacccgc
    11281 tgcttctcgc cgccggagag gtcccgggcg atgtgcatca tccggtgaga gagccggacc
    11341 tgatcgatga ggtcggcggc cctgctgacc gccttctcgg ccggatagcc gatgtcgtcg
    11401 agtgcccgca ggatgttctc gatcacccgg tcatcaccgt agagggcgaa cgtccgctgg
    11461 aacatgatgg cggtccttcg catgatccgc cgtttcatca tcgcgctctc ttcggcccag
    11521 agatcgacat ccaccggctt gagttcgccc ccacaggcag gacacggttt ccctgcctca
    11581 cccgggacgc cgatgtactc gcacttctca catgcgacga catgatagat gatcgcaccg
    11641 cgtgtcgggg gctggtccac accccgcata aggtgcataa gaacggtctt accggccccg
    11701 cttcttccga taataccgac cgtctctccc tctgctactt cgaaatgtat atttttgagt
    11761 acaacgcatc cattgaactc catgcagagg tcctttactg taatgagtgg ggtcatcttg
    11821 agctcctcgt taaactaatt ttctcgtggt atcatattag ggtttatatt gttgcacttt
    11881 tcccggactc cgcggtatcc gtgaggagtt ccgccacaat accgggcacc tcgctgacat
    11941 tccggatcac ccgatccgcg gtggtataga gtatatcggg cttgagatca gactgctgtt
    12001 cggtgagtag cgcgagatcc gccttctgga aggcgtttaa gtcgttgatg gcatctccaa
    12061 ccataacaac tttatcgtac tgctctttga ggtcttccac gagccgggcc ttcatcgagg
    12121 gcgtggcgac gccgtagacc ctttcccggg ggatgccgag gtaatctccc atccgttcga
    12181 gcttcgccac gcggtccccc gacgctatgt atgcggggac gcccatccgg tgcagctcgc
    12241 tgatggcctc tctcgcaccg tcaaacggcc tcccgccggt cgtcaccgtg aactcaatgc
    12301 gggcgaggtc catgtttaag atcacgccgc tgttcatggt gacgatcgac tctctcttgc
    12361 agcagctcca gacctgccgg atacaggcct gcagatcacc gatacgggca tgcctgtcgt
    12421 tgtagaggat ctctgcaacc tcctcggcgg ggatgatccg ccgcgaacag gccaccccga
    12481 agcctatcga ccgctccagg aagaactccg agaggagctg gtcctccggc gcctcgatga
    12541 cgtcccgcga atggaggtga aggacgacga gcactctcgc ctcggccccg aaggtgatgg
    12601 tggtggtctc gacctcggtc agcatctcat cgtggaggag atcgcgtgct acacggtagg
    12661 tcaggagaag tgtgcccgca ctatcgaaaa caacggctac cgacatttgt tctcacgtca
    12721 gtgaactacc ccccgcttgc gcagggggct tcctgcgagt gtccggggct ctcggggagc
    12781 ctcagacctg tttgtcgca
//
LOCUS       DAJB01000197            9852 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_124154, whole genome shotgun sequence.
ACCESSION   DAJB01000197 DAJB01000000
VERSION     DAJB01000197.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_124154"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1684 a         3301 c         3195 g         1668 t
ORIGIN      
        1 gatttaccta cgtcgccctg gaccttgccg ggtaccggag cgggagcatg gacgaggtat
       61 tatgacgacc gaggaccgca gggcggactt cccgatcacg cgggacctcg tgtacctcga
      121 cagtgcagcc acatcgctca ccccgaagcc ggtggtcgag gcgatggtcg aatacgacct
      181 ccgctaccgg gcgaatgtcg ggagaggcgt gcaccggctc accaccgtcg ccacccaccg
      241 ctaccgggac gcccgcgacg ccgtcaggtc gttcatcggg ggagacgggg gcacccttgc
      301 agtgacccgg aacaccaccg aggcgatcgg gacggtcgcc gccggtctcc cctggcagcg
      361 gggggaccgg gtggtgacga ccctgctcga acaccactcg aatctcctcc cctggctccg
      421 gctcagggag cggggagtcg cggtggatat catccggccg gacgctgagg gcaatctcga
      481 cctcgccgac cttgaggcgg ccgtccgcga cgatacccgg ctcgtcgccg tcacccatgc
      541 gtcgaacgtc ctcggcaccg tcctcccggt ccgcgagatc gccgggatct gccatgaccg
      601 gggtgtgcga ctcctcgtcg acggtgccca gtcggtgccg cacctcccga tcgacgtcgc
      661 cgccatcggg tgcgactact tctgcttctc cgggcacaag atgctcgggc cgaccgggac
      721 gggcgggctc tggatgcgcc ggccggacct tgaaccgctc ttcgtcgggg gcggggcggt
      781 cgagagcgtg acggccgggg gctacacgct cgctcccggg gaagagcgct acgatgcggg
      841 gacgccgccg atcgccggca ccatcgggct tgcacgggcg gcggcatacc tcagggagat
      901 cggaatggat acggtgcggc ggcacgagga acgattgtgc gcccggctga tcgacgggct
      961 cgccgcgctc gacggcgtca cggtcgtggg gcaggatgcg gaggctgagc ggatcggcgt
     1021 cgtctcgttc acggtggagg ggatgcaccc gcacgaggtg gcgcaggtac tggacgaggc
     1081 cgccgccgtc ctggtccggt cggggcacca ctgctgccag ccgctcatgg agcatctcgg
     1141 cctcccggac ggaacggtgc gggcgagcac ctacatttat accactccgg aggagatcga
     1201 gatgctgata gccaccgtcg aagagctgtc ccggagagtg atctgatgct ctaccgggaa
     1261 ttgccgatcg tcaaaggcga cggagagacg ttcgtccccg cgacccaccc cgtcgtcgaa
     1321 gaggtgcccc tctccgtgac cgtcaacggg cgccacgcac tcaccgccat gaccagcccg
     1381 tcgatgctcc gggagttcgt catcgggtat ctctacacgg agcggatcgt ccgggaaccc
     1441 ggcgagatcg agtcgatccg gatcgaggga accaccgcga gcgtcctcac gaagaacccg
     1501 ttcgcgatcc tgacctccca caagaccgtc ctctccgggt gcggcgggag cacctcgttc
     1561 ctcgatgccg gacagctccc cgcgatccgg tccgacctca ccctcccggc cgaggcgatc
     1621 cgggcggcga cgaaagagac gctcgactcg gatctgcacc ggatcaccgg gggcatccac
     1681 cttgtcgggc tcttcgatga tgaggggaaa gcgatccgga tcgccgagga tatcggccgg
     1741 cacaacgcgc tcgaccgggt catcggccat ggcctcctat cggggaccga tttctcgcgg
     1801 acgtttgcgg tcagttccgg ccggatctcc tcggagatgg tcaggaaatg ccttgtcgcg
     1861 aacatcccgg tgatcgtctc gcggggggcg acgaccacca tcgccgtcga ggccgcagag
     1921 gcgggcgggc tcacgatcgt cgggttcgtc cggagcaaaa agatgaacat ctacaccggg
     1981 tgggagcggg tccgcggcgc ttcgccgccg tccgccggcg agtgaagcgg ggctctaccg
     2041 atcaagcagc ctgcagacct ggcagacctc gccgctcgta agttcgccgc agatgcggca
     2101 ggtagagagc gccttcgccg gctccgcgga gcgggccgat tccctgaccc cgtcgcggaa
     2161 ctgcataagg cggaccatcg tgcccgggcg gcggtgctca agtcctgcaa gcatcgtgcg
     2221 cacctccgac cggagtgcgg tcccggcgta ggggcactcg ggcaggtcgc ggaagtagcc
     2281 gcggaggagg aggtaggtcg tgatctcttt ctcggagagg acggcgagcg gctttatccg
     2341 cgggacgaag tgccctggct gcccggtcga ggtgtcctgg cgaaggcggg ggaggtcgcc
     2401 gcggaggacg ttcatcagga ccgactgcgc ctcgtcgtcg aggttatggc cggtggcaag
     2461 tttcgtcgcg ccggtccgct tcactgcctc ggtgagcgcc cgcctgcgca gcacgccgca
     2521 gacggtgcag ccctgcgctt ctctcccgac gagtatcgtg tcgaggtcgt ccccgaagag
     2581 gtcggcaaac ccgacgatca cgtgctcgat accgagttca tctgtcaggt cttttgcagc
     2641 cttgagggtc tcctcccggt agcccgcgat cccttcgtcg acggtgactg cgacgagggt
     2701 tgcgcctgat gtagggagga agcggtggag cagcaggagc agggcggtgc tgtcttttcc
     2761 tccgctcagg ccgacggcga tccgnnctcg atgaagtggg cggcgcataa ccgtcgttcc
     2821 ggctcgatga ggcggacgac cgcgggttcg ttgcagaggg agcacaggtt ctctacagga
     2881 gtcaccggct cacccgccgt gagagaaccg gaggatccgg atctcgtcgc cctcgtccgc
     2941 cgtcacgtcc tcggggacga tcgtaccgtt cttcacgacg atcacggtcg tcgggttgat
     3001 cccgagctcg atgaggatct cttctatcgg tgcggagggg caggaaagcg tccgcacgct
     3061 tctgtccggg aggatgatct gcatactgcc cttaacggtt ggtcccgccg ccccataaat
     3121 atcctcgagg gatgggacgg gtgcagccgc aacagcagca aaccataaaa ggatccccgg
     3181 tgatacttag tgagttaaca attgtgaact caacgcccgg aaggagcaga aagtatgcac
     3241 accatgacac cagaggagaa ggcatacctc atcagcatcg gctcggcgag catcgacgag
     3301 gcgctaagcg cagacctcct gccggcgccg gcgaccggcg cgtccgcctc tcgattaacc
     3361 ccgcatcgcc gctccgggtc gttccggacg gagacgggat cgcggtagtc cgggacggag
     3421 agaagatcgt gcggggcagg ctcgaacgcc cgctctacca ttgcccggag caggcctata
     3481 tcaccgtcag cgagcgctgc accttcgact gcaggttctg tccggtgccg aaactcgacg
     3541 ggacggtcaa ggatacggag acagttctcg ggatggtcga agaggccgct cgccggggga
     3601 acctctccgc gatctccctc acgagcggcg tcgcagagtc gccggagcag gaggtggagc
     3661 ggatggcggc cgtggtgaga gcgctccagg cacggttcga cctcccgatc ggggtctcgg
     3721 tctaccccac cgcggactcg accgggatcc tccgcgccgc cggtgccgac gagatcaagt
     3781 acaacgtcga gacgatggac ccggacgtct tcgcgcgagt ctgtcccggc ctctcccttc
     3841 cgttcatcct cgacacactc gcggaggcag tggacgtctt cgggaggaat gccgtcttta
     3901 caaacttcat catcggcctc ggcgaggacg acgagacggt cagagacggc gtcgcccttc
     3961 ttgcgaagat aggcgtcatc cccatcctcc ggccgatctc cgcatcgccc ctccgggcag
     4021 gcgagatcac cgtcaagcgt ccgtcggcgg agcggctcct ccgcctcgcg tcaatgaccc
     4081 gcgagatact ggtacagcac gacctcgacc cgcggcgggc aaggacgatg tgcctcccct
     4141 gcaccgggtg cgatctcacg ccgtttcggg acgtatgagg ccgcgggcat gccgaaggcg
     4201 accgcagtga cccggacccg gggcagagcg gccgatcagg gggtgcatcc atcctcgctt
     4261 tcgtagcgct gcaggatcga gagagccgta tcccggccga gccggtctgc gataagagta
     4321 aggaaaaccg cgaggtgccc gggagagaca catccgaggc tctcgcttcc gtcctgtatc
     4381 agttcgacga ggtattgaag atcatcatcg ttcagctgtt ccagccgttc ccagaattcg
     4441 gcggggtcga ccgcgtagtg gttcgccagc ggcgggagga tggaacgata ggggcctccc
     4501 gaactctcac acatcagccc cgtgcactcc ggggcggttt tcttcaggat catcacgtct
     4561 tgttcacaca ggattcatgc catgggatcg cctctgcggg cccggaaacc cgggccgcac
     4621 cacacattca caattgttaa ggattcatag agataattgt tttgaacagg gccgcgcctt
     4681 tgacactgaa ataaggttgt gataccatct cgccttccag tcccgctcaa accctcgccg
     4741 aacggataac cgcgttgaaa gagaagcgga gcgccgtcat cctcgcccac aactaccaga
     4801 tcccggcagt gcaggaggtc gccgacctcg tcggggactc actcgaactc gcacgggcgg
     4861 cggcagaggt cgacgccggc gtcatcgtct tctgcggcgt cgacttcata gccgagacgg
     4921 ccgcgatcct ctcgccggca aagacggtgg tcctgcccgc ggccgacgca tgctgcccga
     4981 tggcggagat ggtcaccccc ggcgaggtcc gggtgctccg cgagcggttc ccggatgccg
     5041 ccgtggtcgc ctatgtcaac acctccgctg cggtcaaagc ggagagcgac atctgctgca
     5101 cctccgcaaa cgccgtcgcc atcgtcgagt cgctcgcggc agagcaggtg atcttcctcc
     5161 ccgacagaaa cctcgcccgt tacgtcgcac ggttcaccga aaaagagatc ctcccctggg
     5221 acggctactg tatcgtccac gaccggatga cggccggtga ggtgacagcc gcccgccgtg
     5281 ctcacccgga cgccgaggtg ctcgttcacc ccgagtgccg gcccgagatg atcgaccttg
     5341 ccgatcacgt cttctctacc tcggggatgg tgcggtacgc ctgcgcaagc ccgggacggg
     5401 agttcgtcat cggcaccgag gtcggactcc tccacccgct cgaaaagaag tgcccgggga
     5461 aaatcttctc ccccctctcg aataaggcgg tctgcgtcaa catgaagaag accgatctcg
     5521 caaaggtgct tgcggccctc gagcggctca atccgcgggt gacggtcccg gaggagaccg
     5581 ccgtccgggc gcggacggca atccggcgga tgctggacct tccccggtag ggccgacttc
     5641 agaattccct gagaacatcc ctctggctga aaaaccggtc ccagtcccag ggctcgtcga
     5701 gcacccggac ctcgacgtcc cggacgccgc tcacgtcgag cacccggcgt cggacccggt
     5761 cggtaaggta gtcgacgagc gggcaggcgg cggtggtcag gaccatctcc acgagcaccg
     5821 aggagtcctg aacggtgatc cccttgatca ggccgaggtc gacgacgtcg atcccgacct
     5881 ccgggtcgat cacttcttta agcgccgccc ggatactctc ctcgatcgcg atctcctcct
     5941 gccgtttacc gccaaccagt cccgaccgct ccaccgcccg gagccgctcc tcgatccggt
     6001 tctgccggtc gagaatgcgg ctgaccaccc ggagcatcgg gtcggggagg tcgccgcggt
     6061 cgagtctctc ctggcccttc cggcacttcg ggccggcgag cctgcccgga acgcccacga
     6121 ccgtcgcacc ggcagggacg gggtggacga cgaccgaacc cgccccgatc ttcgccccgc
     6181 gcccgacggt gatgggaccg aggacactcg cccccgaccc gatgacgacg ttatcctcga
     6241 tagtcgggtg ccgcttcgtc cgctcgagcg cggtgccgcc gagcaccacg cccatgtaga
     6301 tcaggacgtc gtcgccgacc tcggcggtct ccccgatcac gacgcccgat ccgtggtcga
     6361 tgaagacccg gcggccgatc ttcgcgccgg ggtggatctc gatcccggtc agcgcccgcg
     6421 agatatggga gacgaaccgg gccgggaagt agagccggcg gcgatagaag gcatgcgcga
     6481 tccggtgcgc ccagatggcg tggagacccg ggtaacagca gatcacctcg agcgtcgacc
     6541 gggctgccgg gtcttttgca aagactgtct ggatatcttc cctgatgctc accgcgtccc
     6601 gtccccctac tgcgcctcga agagatccgt cgagagatac cgctcgccgg tatccgggag
     6661 gatcacgacg atnnaggatc ccctctttgc gggcgagcag gcggctcatc gcgaaggcgt
     6721 ccgcggaggt gacccggaga acctcgtcga cgagatcggt cctgagaacg cccggcacga
     6781 aaccggcccc gattccctga atgcggtgcg ggccggccgt tccgccggag aggacgggag
     6841 actccgccgg ttcgacggca attgcccgga aagagggtct tcgtgccttg atgacctcgg
     6901 cgattccggt gatcgtcccg cccgtcccga caccggcgac gacggcatcg acggtcccgt
     6961 cggtatcccg ccagatctcc tcggccgtcg tccggcgatg gacggcgggg ttcgccgggt
     7021 tgtcaaactg ccggggcacg aagtaggcgt tcggcttccc ggcggcgatc cgggccgcac
     7081 ggctgaccgc ccccgtcatc ccctcggctc cgggcgtgag aaccacctcg gcgcccagcg
     7141 ctgcgaggag cttgcgccgc tccacgctca tcgtctcggg catcaccagc acgagcgggt
     7201 aaccgcgggc tgcacagacg aacgcgagtg caatcccggt gttgccgctc gtcgcctcga
     7261 cgatcgtcgt cccctcccgg atctcaccgg ttcgttcggc ttcatcgatc atcgcgaccc
     7321 cgatccggtc tttcacgctc cccatcgggt tgaatgactc caccttggcg aggaccgtcg
     7381 cccgggagcc ttccgtcacg cggttcaatc ggacgagcgg ggtgcccccg atcgtccagg
     7441 tgatatcatg gtatatccgt cccatctgca tcaatctcct atccgttact cacgcatgtt
     7501 gtgggccggc cgcatcctga gcgtttccac gtccccggca tctcccaccg cttcctccag
     7561 ggtactgacc gtctcgatcg ttgcagcggt ggaggggcgt gccggcaccg ctccgcaccc
     7621 gcaggcaggg gcgatcgacg catcgaacgt cccgatctcg cgggcgagcc ggacggcatc
     7681 ctccttgtcg aacgcgatca gcggccggta gaccgggatc gtcgccgcat cggtgagcac
     7741 catcaggttg tcgagggtct gcgacgcgac ctgccccatc gactcgccgg tgacgaaccc
     7801 ctttgcccct tctctccggg cgatctcggc ggcgatccgg tacatccgcc gcttgcagag
     7861 gaggcaggtg tagcgctcct gcccccgttc ctcaagcacc cgccgtgccc gggcgaggta
     7921 gccgtcctcg acgaccagga gttcgatatc cggctggtag cgccggaggg cctcgaccgt
     7981 ggcctctgtc cgggcgaggt tcgtctcatc gagatagcct tcgagcccga catagagcgg
     8041 gacgatccgg cacccgcgct tcatcatcag ataggccgca accggtgagt cgatgccgcc
     8101 ggagacgagc gcgacgagcg ttccctcgac gccaagggga atgccgccga cggccgggat
     8161 cttccggtcg aagaggtagc aggtcccgtc ccggacctcg acgaagatct cccgtcccgg
     8221 gtcgtcgaga tcgacgcgga ggtgcgggta ctccgcccgg accaggttcc cgagccgttc
     8281 tgcgagatcc ttggaagtaa atgagtgctt cccgatgcgc cggatccgga gggcgaaggt
     8341 cgcagcctct gcgataccgt gacgctcgca gaacgcgagg agacccttcg ggagatcgtc
     8401 gagcggcacg acgtcgcaga gcgagaacga gacgatcccg aagacgttcc tgagcacctc
     8461 cgggtcgacc tccccggcaa gccagatcct cccgcgctcc cgccggggtt ctgcacccgg
     8521 aagcacccgt ttgatgttcc ggataagggt cttctcccac tcccggcgga cgggctccga
     8581 cttgaggaag acctcggagt agcgcaccag ccagacctgc tctgtatcga tcatcgcttt
     8641 cctccgggtc tgcccttctg cctgagtcgt tcgacctcct catcccccgg cacccggatc
     8701 aggaatgtcc cgtggcaggg tttggtgaag gggaagacga tgaaggtatc ctccaccccg
     8761 acggcgatcg gcggcacccc gatgatatgc gtatcgaaga tcgcgtaccc cgggtcgacg
     8821 tagtagacct cgcggcaccg cttctccacc gccgtccggc actccgcaaa agaggctccc
     8881 cggaggagga tctccgggtt caactcgtcg atgcacgcca tccgaagacc tcgtcaatgc
     8941 ttttcttcag ttttcccggg ttgtggaccg ccttctccac gaccgtttca cagggaaaat
     9001 caacctgcgc cgcgagctct tcggcgttcc ggccgaagac gacgacgacg agacgcgctc
     9061 ccgggagcag gtagcgcatc gaggcggcgt acgagatgcc ggcatcgtta tgggcgaata
     9121 ccacgcagag gccggcgtct ctcttcccct ctgcgacctc cccgatacag cggtcgaggt
     9181 cgacggtcct tccgatgtag cgcttcttcc ggtcggagac gcggaggaga ccgagaaccg
     9241 aggggtttcc agcactgagg acgtcgtagc cgtcttcact gagccggcct gcaaggtaga
     9301 ggccgatcgc cgcctgcacc ggaacctccg ggcacccgaa gaccaggagg gcctgctcgt
     9361 tcttctgtaa ttcattctgg gtcatgcgca tcgtcctatc agcgtgctga tggcatcgct
     9421 cgatatgagg ccgatcaccc gctgatcggc gtcgacgacg ggaagggcgg atatcgaacg
     9481 ttcttccatc cttcgagcgg cctccgtgac cggttcgtcc ggcgcggcgg tgacgaccga
     9541 acgggacatg atctcgtcga ggctcccgta ccggcaggcg accgctttcg cgatgtccca
     9601 ggaggtgacg atcccggcaa ggtcgccccg ctcggcgagg acggggaggt ggttgacggc
     9661 gttctcgatc atgatccttg ctgcgtcgtg aatggacgcc tcctcagcga tggtcggggc
     9721 gtccgtctcc atgacgtccc ggacgagcag gtggtcgagg aacgtgccga tgaggtagtt
     9781 catctgcccg tcttcgatga ggaaggcatc gtgcccgtgg ttcgaccgga tctcgcagta
     9841 ctggacgttg at
//
LOCUS       DAJB01000198            2645 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_96162, whole genome shotgun sequence.
ACCESSION   DAJB01000198 DAJB01000000
VERSION     DAJB01000198.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_96162"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          473 a          949 c          815 g          408 t
ORIGIN      
        1 gggcctcctg tgcgttgatt gtttcgtcgt ccaggaagac aaggttgccg ccgaggtcca
       61 caatggcctc gatgagcccc gcctccccgc gcttctttgc ggtcgtggcg tagacgagga
      121 ccttctcagg ggctaccccg gcctcccgga gcgcccgccc gatcgcctcg atcacctcgg
      181 ccgccccgac gcccttccgg cacccgacgc cgacgacgta ctccccgttc cggacgagga
      241 gggagacacc cgggcccgcg acgacgatcc cgggtccggt gacggcgtag agaggcacgt
      301 cggcgtccag caccgcagcg ttcaccgccc gggtggagtc gcggttcacg atatcgcagc
      361 ctgtccgaac ggctatcccc tcgaccgact ccctcccccg ggtctcggtc gcggtcgtga
      421 tcgccggttc gatcccgagg acggcaagtt cccgggcaag gtcgtttgcg ccgtggtgcc
      481 cgccaacaac cggtatggcg taacgcaggt ccggcccgac caccaccacc gccggatccc
      541 gccacttatc cgtcaggagc ggggcgatcc cccggacagc gatcccggcc gacatcaggg
      601 cgacgatccg accgtaaccg gcaaacgccg cccggaaagc ctccgggtca taggggatga
      661 cttcggcgtc gagcaccccg gcgatcctcc gggcgtcggg gaggaaccgc tcgagcgcta
      721 tcacggccgt ccgggtcatg agtagaggac cgacctcccg aaccccgacg ttgctccttc
      781 gaccaccttc ccgatgacga tcagtgccgt ccgctcgatc ccggctgccc gggccttcgc
      841 ggcgatgtcg gccaccgtcc ctaagacgac cttctggtcc ggccaggagg cgtggtagac
      901 gaccgccgcc ggggtcccgg gcgggcactc cacccgggcg agaatatcct ccattcgctc
      961 ggtcccgagg aagaccacca tcgtctgccc gagccgggag aactcagcga tccggtcctc
     1021 ggcaagcgtc gcccccgccg ggcgggtgac gatcaggctc tcggagacgt cgcggagcgt
     1081 gaactgtgtc ttgagcgccg ccgcggccgc aaagagcgac gagacgccgg ggacgatctc
     1141 ggcctcgatc ccgcgccggg aaagttcggc catctgctcg acgatcgcgc catacagcgc
     1201 cggatcgccg gaatggagcc ggacgacaag tcttcccgcc cggatatgct cctcgatggc
     1261 atcgacgatc tccggaagcg tcatccccca gctgtcgagt ctcacgccag cgccggactc
     1321 cgcaacaagc gccgggttca cgagcgatcc cgcgtagatg aggacgtccg cccgccggag
     1381 gagatccatt ccccggacgg tgatgaggtc gggcgcccct ggccccgcac ccacgatgta
     1441 gactttatgc atgcgaccgc ctcgcaaaga ggatcgagaa atagtcgctc tcctcgggga
     1501 gatcgtcccc ccggtagacc cgctgctccg ggaggaacat ccgctccacg aggacgaact
     1561 ccgagtagcc cttccccctc aagtcggccg cgagcgccgc cggtcgccgg accttcatga
     1621 agatccccga ctcaggtccg ttcccgtcag agacaaaaaa tccgccggcc accgggacgt
     1681 ttgccacgga ggcgaacgcg gtgatggaac tgatcccggg cgtggtcgca aacgtgacct
     1741 ccgggtagcg cccggcaatc acctcacaga gccgggagaa ggtcgagtaa aagttcgggt
     1801 ccccgatgat cccgaagacc gcgagaccgt ccagcgcgaa cggtgcaatc cggtccgcgt
     1861 tctcttcaag gcattcacgg atgtaggcct cgtcgctcgt cattggaaag ttcaggacga
     1921 cggcatcggg gcggtaaggc cgcaccagat cgtaggcgag gtttccggga acgaagacca
     1981 ccgctgcttc atgaaggagc cgaaccgccc ggagcgtcag aaggtccggg tcgccgggcc
     2041 cgagccccac ccctacgagc acccggcacc cccgacgatg acatagacgg gattgatggg
     2101 tttaaacatc accccgccgg cgatgccggc ggaccgggag acctggaggt ggacggcctc
     2161 cagaaagatc ccgaggcgct gcatactcgt tatcgcctcg gagaccgtcc cgagcatcac
     2221 cgcgttcacg acgatcctcc cctgcaccct cccggcgagg agatcgagga cctccggcag
     2281 gcggcgggat cccccaacga aggcagcgtc gaggtggtcg ataccctgga ggatctccac
     2341 tgcgtcaccc tcgaagaact cgacgttccc ggcgccggcg gcagccgcct cctcccgggc
     2401 gtacgcgacc gcctccggtc tcctgtcgat tgcgtagacg tgttcggcca ccctcgaggc
     2461 agcgatggcg atcttcccgg tcccgcaccc gatatcggct atccggtcac ccggacggat
     2521 gccgagtttc gcgagcgaga cagccatgat ttcgtcctgc gtgggtccac ccttcagtcc
     2581 catagcatcc tcatttccca gataaatttg tgctaacatc tagtcaacta cccccggtta
     2641 aaacc
//
LOCUS       DAJB01000199            6658 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_84737, whole genome shotgun sequence.
ACCESSION   DAJB01000199 DAJB01000000
VERSION     DAJB01000199.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_84737"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    857..971
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1164 a         2073 c         2022 g         1284 t
ORIGIN      
        1 aggaggcgat caacggccgg tacggtgatg tcatctttgc gacggatagc ggcatctccg
       61 tgtacacggc aaacagcacc tggtattcgg tcaacccgag gtacccggga gagacggcct
      121 acggtctgct cgcccccctc gataccatgg tgaccgcgat cgccctcgat gcggagggcc
      181 acctctgggt agggtatcca aacgggctcc agatcggaga cggggcggga taccgggaga
      241 tccaggacca gcagttcctc aagaaccgca acatcaactg catggtgcga tggggggacg
      301 agatgtggat cgccaccgga cgggcgggcc tccaccgctg ccatggcgat gactggacat
      361 ggtataagcc gctcgggccc gaaggcctcg ggtgctacac cgttgtgagc atggtggtgg
      421 atgccgcgag cgatgcgctc gtcatcgggt ccgaaagcaa tggcatccgt gtgctcaagg
      481 atcggacagg ggaggtccgc ttcgaggagg tcacctgccg cggtgatccg ctcaggggga
      541 ttgccggggt gcgggtcgac ccgttcgggg gcgtctacct cttcaaccgc acgacggtcc
      601 tccggtatac ccctgatgcg ggggttaccc cggtcctcgg tgccggggac ctgagcacgt
      661 tcccggtcgt catcaacgat atcgcggcaa cacccaccgg gatgctgttt atcgcgtcgg
      721 ataacggggt ctacgggtgg aactcgtcgg gtgtcgccct gcatatcacg tctggagacg
      781 gccttcgctc aaacatcgtc aagagactct tcgtcgatgc tgacgggcgg tgctggttcg
      841 ttgttcccgg gaacgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn ngaggcgtgg atcagggcgg ggataccggg acggcaggtg cctctgacta
     1021 tcgccatgcc gcggtcagga agcacaggcg tgtttgagcc ggccggcctg taggtttgtt
     1081 tgggtggatt ctagaagagt gaaccttcag gactaccacc agcagttcac cggaaaagaa
     1141 gaggagttcc cgtgccgctc acgacttcga gatctccgcg tagtagtatt ccccggccgc
     1201 gacctgctcc cgatccagga acgaccccgg cttgttgatc cgcggcctcc cggtcctgtc
     1261 ccgccggaag gagatcccga gcgcttttaa gaaccggttc atgcccggcg ccatatcgaa
     1321 cggcccggcg gccttgccgt gcagacccgg ttcgccctcg aagaccagaa tacggtcgct
     1381 gatcatatcg atcacgtaga tgtcgtggtc gatcacgagc gtgctcgcgt cccgcccttc
     1441 ggcgtgcttc ctgatcatcc tcgagatctt cacccgctgc tccacgtcga ggtgggcgct
     1501 cggctcgtct aagatgtaga ggtcggcgtc gcgcgagagg cagattgcta tcgccacgcg
     1561 ctggagttcg ccgccggaga gcgtgttcac cggggcctga agaagatgtg caagcgagag
     1621 gggctctaag atctcgtgct ggtagaacga ggtgtcgaac ttcgtcgtcg tctgccggag
     1681 gacctcttcg acggtcgcct ccgagtcgcc tttgaggtac tgcggcttgt aggatatccg
     1741 cacccgggaa tccatcgccc cggtatcggg gggaagcacc ccggcaagga gctgggcgaa
     1801 cgtgctcttc ccgataccgt tcgccccgag gaccccgagg acctcgccgc tccggatctc
     1861 cccgccgtcg acggtgagcg agaactgttc gaaacgcttc gtcatggccg ggaacgcgag
     1921 gagcacctcc cggttcgcct cggcggcgtg ggcccgggtg tcgaaggtca ccgcatacgt
     1981 ccgtatcctg acgttctcct ccggcaggaa accctccagg tactggttga tccctatccg
     2041 gacacccttc ggatgggtga tgacaccaaa gaccgccggt tctccgtagg cgatatgcac
     2101 ggtatcggca agcatatcga ggatggcgag gtcgtgctcg acgataacca ccggccgctc
     2161 cagggcgagt tcccggatca ggttcgcggc ggccatccgc tggtagacat cgaggtacgg
     2221 cgtgatttcg tcgaggaaat agaagtcggc gtcgcgcgcg aggcaggcgg tgatcgccac
     2281 ccgctgcagc tcgccgccgg agagggtcga aataggccgg tcgatgaccg ctctgagcga
     2341 taactggtcg atgtagtagt cgagcctgcc gcgctcatcg gtggtcgcaa gaaggtcgcg
     2401 caccgacccg gtaaagacct tcgggatctg gtcgatgtac tgcggcttca cggcgacctt
     2461 catgttcttc tgcgagagca tctggaggta gtcgaagagc tcggttccct ggtagagggt
     2521 gaggacatcc ttccaggaca tctccgcatc aatccggccc aggttcggga cgagcgtgcc
     2581 cgagaggatc ttcaccgacg tgctcttgcc gataccgttt ggcccgagga taccggtgac
     2641 cttcccttcg atggggatcg ggaggccgta gagagcgaaa ccgttctgac cgtagcggtg
     2701 gatcggctgg tcgagttgtt cggggagatt gacgatgtca agggcttcga acgggcactt
     2761 cttcacgcag atgccgcagc ccacgcagag ctcctcggaa atgatgactt tctggtcttc
     2821 accgatgacg acggtctcgt cgccggtccg gacacgcggg cagtaaatga tgcattcggt
     2881 gccgcacctg gccggatgac atcgatcacg gtgtacaaca gcaatacgca tgggtgatca
     2941 cagaacgctg gtggttaaga gaatagtcca ggtcatgaac cagagcgcga acgtcatgaa
     3001 actctggtaa aaccagtctt ttttgttcag ttccatatag tcgatccgga tgagcatgaa
     3061 gatgtgtttc tggaagacga cgcccgccat caggaggaga acgccgatga tggcgttttc
     3121 ctggagccca ctgaccgggt caggggtgcc ggagaggtag aacgagagcc ctccagccag
     3181 gatgcccatg aaacaggcta caagcgtccg ctttatccga cctctgtgct cgacctgttt
     3241 ttcggcacgt gtccgcggcc gtgtcttttc aacctctgct gctaccgcat tatcgctcat
     3301 cgtgacacca gtgttacgcc attcctggaa gggaagtctc tccgggacgg cggctcccgg
     3361 ccactccctg gattgacgga attattacat tatagggtct tctccggtta taaaggtagg
     3421 caactcccgc cggacggtct cactcgatcc tgtaccgcag gagtgctgca gcgccgccga
     3481 ttgccgcgag acgttctcca ggctcgaact cggtgctcag caccacgacc gatgcgttga
     3541 cgcgctcggc ctccctgatg acgttcgtag ccccctcgtc ccggagcagg gtatcggaga
     3601 cgagcaccgt ctccgccgca ccgtaagcaa ttgccttcct gacctcgtcg agaccgtagg
     3661 cgacagcgcc cccggcagcg acccggtgca ggacttcgtc catcagcctg acctcgcgtg
     3721 cgaggtggag gtcgccgagg agccgctcga ggaccccctg cccgatgacc tcctgcacgg
     3781 ccccccgccc gatacgccgc gtctcgacga cgagcatacg ctctgcgagg tcggacgcac
     3841 gggctttcac gtaattcgca aaatcgtcct tcacgaaccc cgggcccgcg acgaccaggg
     3901 ggccggtgac cgcggccagc acctcaagcg tcttctcgaa gagcaccgac cgggtgccgg
     3961 tatcagtacc cttgctgctc ccggccgcaa cagtcgtcac ccactcaggg ccgaactggc
     4021 gcaggcggta gacctgcgcc tccccctcct cgacgctcac gacgtggacc acgccgtatg
     4081 cagaggcatc gacggcccgg tgtatccgtt cgcagtcgac ggaccgccac tgtttgatga
     4141 ccgagatctc gaaccccgcc tccacgttca aggtgtggtg cgcggtgacg tccactccgc
     4201 tctcaatgat cccggcgata cggaggcggt tcgtgtgctg gtggaactcc accttctcga
     4261 cccggatccc gagccggacc ggccgcttct ccaccttctc cggccggagc ttatcggttg
     4321 ccgcctccac gctccggaag gtcgtcgcaa agacgagatc ccccggcccg accaggtggg
     4381 agagatgcca gatgtcgtcg agcgtctccg ggaagaggcg tatctcgccg aacttccccc
     4441 gcatctcccg aacttcagcc ttcatggttc tacgatatcg gagcctcccg gcgggacgaa
     4501 tgcggcgacc gtctcggtgt tcagggcgtt cttgagccct ttccactcgt cttccgagag
     4561 tttatccctg agtccccgga caaccttctt tgcggtgtcg ttcggctcga actgccccgg
     4621 acgaagctcc agccatactt tcgcacgccc ggtgacggcc ccgggcgggc cgccgatgac
     4681 ggcgacctcg ggcgccatcg caagaccgat cgccaccccg agggggacgt tcttaaagta
     4741 ctgccgctct ccccggacga tgaaggcgcc ccgggccaca tactcgccgg actcgggcgt
     4801 cttgctcacc tggtcggggc gggcggcgta gacgtccgcc gtgaagtgcc cggccttcca
     4861 ggcgttggag taggatgcgg cgaactgcac ggcctcgtca agatgttcgg tagcgccttt
     4921 cacgatgacg acgctcccgc cgtggacgtc ggcgtggacg aagagatccc cgccctccat
     4981 gtatttcttg acgagttcct cgttctggga ggcgtcccgg ccgccgatga cgagggtccc
     5041 gtcggtggtg gtgaaccagc ggaaccggtg gtaccaccgc ttctttaaga gggcaaggtt
     5101 ctgtttatgc cggggcttct cggggaccat ccgctccatt gccgcgagtg cgcccgtctt
     5161 cttcttctta aacttcttga tcaggtcgta gtagcggccg aggttctgct ccatcgtctc
     5221 gtggacgtag accttcaccc gctcctcttc aagaccgagt tccaccgccg cctcggcagg
     5281 gtggaccgca cggaccatct ttgccgttgg gttctcaccg ttcgatttga ggatctgctc
     5341 gatctcctgc cacgagcggc ttttgctcgc ctcgtcgagc gtcgttaaga tcccggcaac
     5401 gggcgtgtag ttctcgtaga gcacctccac aacccgctcg taccgctcga tcttcttctc
     5461 aaaccccttg atcgcctccg cctggcgccg gcggatcacc tctgccttgg acaggcgggg
     5521 tttttcggcc gcggcctcct ccttttgacc cgtcaccttc gggtagaacg catccagggc
     5581 ctcgctgaag gtcgcaaacc gctcgcgaac ctcttcgccg gcaagcacca ccggccagca
     5641 cccggacggc gttgtcaccg gctcccggcg cctcccgacg tcggccatca gacgctcgaa
     5701 agctgcccgg atcgcctccg cgtccacctc tgcggcggcg gcgcccttat cgacgcctgc
     5761 catccggcag acctcttcgg catacgcgcc gccgagcatg cacccgacgg caagcgtccg
     5821 gacgatgtcg cggtcggact ccgcgagcat cttctggaac tcaacgggag ggagcgccga
     5881 gcagtcggtc ccctcgaagg ggtagactac acccggaata atctcccggg tcttgaaccg
     5941 gtggtgccag aggggcttga tgatcgtata ctcttcgttg cagaggatcg tgttcccctc
     6001 atcgaagagt tcgaagatga ggtgataggt cgtatccctc ttgccgatat cgatgctcat
     6061 ggtgcgctcg agcccgagct ggcggatgct gagcaccttc ccaccgtcga gatgcttccg
     6121 gagcagcatc gcataactcg gcgggttctt tgggggcgtg ggaaactctg cggtgaagtg
     6181 ggcacgtcgt cccatctcca cgagcagcag gtattttgcc cggtcttccc cgtttaacct
     6241 gataccgagg gtcttcgcgt cgaactggta gatcttgccg atccagagcg gaaggcgatc
     6301 ggcagcctcc gccaccagcg cccgaagatc gaccccgctc attccctgta gtgtcgccat
     6361 tgattccaga atacattcgg cccggtacct aaagaagggt actgccggca tgaaaccctg
     6421 gttttgccgg gaattggatg ggtgcgctgt gcccgagggg tcggcgaaga ggtgatcgct
     6481 gcactcgata acctgcagaa agccctccag atttctcacg acccttcagt cgcaccctgc
     6541 acctatcggt ttgagcactg aaggtgcgct ttgcggggac aaaacagtcg gaagcgatga
     6601 caaagacccg tatactggac catggggata tcgccccccg gggaggggct gacgggga
//
LOCUS       DAJB01000200            4006 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_17453, whole genome shotgun sequence.
ACCESSION   DAJB01000200 DAJB01000000
VERSION     DAJB01000200.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_17453"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          833 a         1143 c         1242 g          788 t
ORIGIN      
        1 ctaatcactc aggacgacat tgctgtcatt aaaggatggc ctccctaccc tgctgaattc
       61 agcgatctcg attacgcgct cagagacagg ggctggctgg atgaatactc gcaaaaaccc
      121 gatgcggata ttcttatcgc cgacgatgaa ggaacgattg ccggtttttc gatcattgca
      181 cgcgagcccg gcggcattgc cgagttccgg atcgcactcc acccggagag atgcgggcga
      241 gggttaggga gaaccgtcac acatctctca cttgtgcatg gattttccga tactacaacc
      301 ggcaccatcc ggcttattgt aaggaagaat aatccgcgtg caaaaagact gtatgaagag
      361 atgcacttcc gaaataccgg cgagtgcata gaggagatcc agggaaaacc tgtggagttc
      421 tatcggatgg agatcaacag gattgcattg tacggggcgg atgcatgatg aaggaggcat
      481 tactggtcat cgatgtccag aacgaatatt tctccggcaa tctgccgata acatacccga
      541 agacgagctt aatcagtatc cttcaagcga tggatgccgc atgtgctgca gagatcccgg
      601 tcgtcgtgat ccggcacacc agcactgctc cggatgcccc ctcgttccgg ttcggaaccc
      661 cggcatggga gcttcatccc gaagtgaaga agcggccgta tgatctgctc atcgagaagg
      721 ccctgcccgg gagtttcacc gatacaggcc ttgatgcctg gcttagagat cggtccgtca
      781 attcggtgac cattaccggc tacatggccc agatgtgctg tgattcgacc gcccggcagg
      841 cattccataa cgggtacggc gtgaagttcc tttcggatgc caccgggacg ctctccgttg
      901 ccaaccgggc cgggacgatc tccgatgccg accttcaccg ggcagtcctc gtgacccagc
      961 agatgcgatt ctcgcaggtg atgacgaccg cagaatggat ccggtcgctg gaacttgcat
     1021 atgggtagcg gtattccgcc tgatcgcgtc cgagggatcc ccgtggacac cgctcttctt
     1081 ctccttcgag tggccgccgg tggtgtatgc actggacata ctcgcgtggg acgttttctt
     1141 cgccctctcg atgctctttg ccgctcccgt attcgggacc ggccagctgg aaaaggcggt
     1201 ccgggtccag atggtcgcga gtggagtatt gagcctcgcg gggctggccg gcgtgccgct
     1261 tggcgacatg ggtatccgga atatcggggt cgtgggctac gtcggggttg gtctggtcgt
     1321 gttcctcctg cttggggtcg tcttcgggcg tgctcaaccg gcgcccggag aggtcgggtg
     1381 aagtccggtt cgggatcagg gatctgcggt aaaactgcct cacgacgacg ctccccggca
     1441 aaccttgaac cccctgtaca tcctaccctg atccagatac aaagacggga gagagcatga
     1501 acaagacaat cattaccgtc gtcggaaagg ataccgtggg aattatcgcg aaggtctgca
     1561 cgtatcttgc caagaacgag gtcaacgtcg aggatatctc gcagacgatt gtgcaggggt
     1621 acttcaatat gatgatgatc gtcgataccg gcgggtcttc aaaaccgtat gccgaaatgg
     1681 tgaccgaact tgaaggtctc ggcgatgaga tcggcgttaa aatccggtgc cagcgggaag
     1741 atatttttac gaaaatgcac cgtatctgag gggttttatg gtcactattc ttgaggtgaa
     1801 cgagacgaac aagatgatcg agcaggagaa gctcgatgtc cggaccatca cgctcggcat
     1861 cagccttctt gactgctgcg actccgatct cgatgcctta aaccggaaca tatacgacaa
     1921 aatcacccgg gtggcagaga acctcgtctc aaccgggagg gagatcgagc tcgagtacgg
     1981 gatcccgatt gtaaacaaga gaatatccgt aacccccatc gcacttgtcg gcggacgggc
     2041 ctgcaggtcc ccggaggatt ttgtagaggt tgcaaagaca cttgatcggg ccgcacggga
     2101 tacgggggtc aacttcctcg gggggtactc ggccatcgtc tcgaagggca tgaccccggc
     2161 cgatgaagcg ctcatccgct caatcccggc ggctctctcc tcgaccgaga gggtctgcag
     2221 ctcggtcaat atcggctcga cgaagaccgg gatcaacatg gatgccgtaa aactgatggg
     2281 agagattgta cgggagacgg ccgaggcgac gaaagagaat aactcccttg gctgtgcgaa
     2341 actggtcgtc ctctgcaacg cccccgacga caacccgttc atggccgggg cttttcacgg
     2401 cgtctccgag gctgatgcgg tcatcaacgt gggcgtaagc ggcccgggag tcatcaagca
     2461 tgcactcgag ggtgtccggg gaaagaactt cgagattctc tgcgagacgg tcaagaggac
     2521 ggccttcaag gtcacccgtg cagggcagct cgtcgcccag gaggcgtcgg agaggctcgg
     2581 gattccgttc gggatcgtgg atctctctct tgcccccacg ccctccgtcg gggacagcgt
     2641 cgctgagatc ctcgaggaga tgggactcga gtcggtcggc gcaccgggga cgacggccgc
     2701 acttgccctc ttaaacgacc aggtgaagaa gggagggatc atggcgagtt cctttgtcgg
     2761 cggactctcg ggcgcattca tcccggtcag cgaggaccag gggatgatcg acgcggtgaa
     2821 ccgcggtgcc ctcaccatcg agaagctcga ggcgatgacc tgcgtatgct cggttggcct
     2881 cgatatgatt gcgattccgg gcgacacacc cgcttcgacg atatccggta tcatcgcaga
     2941 tgaggcggca atcgggatga tcaacaataa gacgaccgct gtccggctga tcccggtgat
     3001 aggaaaggat gtcggcgaca ccgtcgagtt cggcggactg ttaggccatg caccggtgca
     3061 gagggtgaac aggttttgct gcgccgactt cataaaccgt ggcgggcgga ttcccgcacc
     3121 gattcacagt tttaagaact gagtgccggg ataccccctc acccggtctc gaaactcatg
     3181 acgccgtagc actcatcgag cgggaggtcg gggatctcct ttgtgtacat ccgcgccgtg
     3241 ccgaagaccg ggaccgtccc gtgccgctct gcgagcagaa caccggcgcc gttcggctcc
     3301 ggcacgtcga ggaagagcgg ctgaccgggc agtgccgccg caaggccgga gtagatccgc
     3361 tcggcgatct ccggcgtgtc ggcaaagagc gggccgatct tcgcgcccct gcggcacggc
     3421 cgcacctgcc cgtagccgcg gacctccccg tcctcgagga cggccagcgc cgtcgcgccg
     3481 ggctgcgcca gccagtggcg gaggaactcc ggccgctctg ccgggaaatg gcgtgcgtcg
     3541 tagcggacga gggcgtcgaa gggaacgttc gcggcgtccg cgagacccgg aggacaactg
     3601 cccccgccga gaccctcata cctgatattc cggtgcgaga agaggaagcc gtgggctctg
     3661 tagcggtcct gcatctcgaa gaccccgtcg atcccgaagt tgtagggccc ggcgagccgg
     3721 tcggcgcact cctggatcgc aagaccgata ccccggcccc gatattcagg gtccaggatg
     3781 tagaacccgc cgaaggcgaa ctcgtccggg tagaccatga gtcaactacc cccggttaaa
     3841 accgggggct tgcagctaga atcatcggag atgcttctgc taccataggc tggttggcgg
     3901 cagccctgaa ggcaatgttt ttcgcagcat ttgtgtcagc aggttcggtg tggccgcacg
     3961 ccctacacca aaagaggttt ctggttttcc ggtttttccg gtcaac
//
LOCUS       DAJB01000201           10913 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_82566, whole genome shotgun sequence.
ACCESSION   DAJB01000201 DAJB01000000
VERSION     DAJB01000201.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10913)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_82566"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2089 a         3248 c         3367 g         2207 t
ORIGIN      
        1 ctgcgaaagc ggggggtagt tcactcctgc cgtaacggcc ggctacctct actgcgagag
       61 ggggatcgtc gccgccaccg tgctcgggct cgcctgcctc ggcatcacgg cgctccgcgc
      121 ctaccctaat ccggtcatat tcctcccgtt catcgccatg gttgctcttg cgtcgctcgc
      181 ctcgaccgtc gggcactcga cccgggaacg ggcccgccaa cgggaggcca tgggacaggg
      241 tcccggcggt gcgtttctcc tcaggcgcga gaccaggacg attgcggatg cgaaccagga
      301 cttcgcggag ctcctgggct atgcgcggga agacctgcag gagatgccgg cttcaaggct
      361 ctggccgtat gccgacgacc gcgaacggtt cttcgccctg gcaaaaccgg gtgagggcag
      421 tacgatcatc gagacgcagt tcgtcggccg ggacggcaaa acacactggt tcgtcctctg
      481 ggggcgctgc atcgacgatg ccgtcatatc ctgccgggtc tcggatatca cccggtacaa
      541 agaggcggaa gcagccctga acgccgaaca ccgccgcctc ttctcggtcc tggataccct
      601 ccccgcctat gtcaccctgc agagagagga ccacaccttc cggttcgcca acagggcgtt
      661 ccgggagacc ttcggcaacc cggagggcag gacctgctac gaggtgcagc aaggctctcg
      721 caggacctcc ggacctgcgc tatccacgcc atgccccgcg ctgcgttcga gaccgggcag
      781 gtagaccaga tgctcccgcc ggaaggaatc cgggagagaa tcatccagat agcgggaatt
      841 ctctgatata ctcttctttt gagggccttc ccgggacccg ggcctcccac ccgggaacgt
      901 gccctctaaa tgcgtgaagg gcgcatactt caggtatgga ggtctcacct gctaccgccc
      961 ggtacttcga tgaactgata gccgggcttc tgtccgcaat gcagctcgcc gctgccgcac
     1021 gggcccgcgg actcgatcca aggacggaga tcgagatccc tatagcaagc gacctcgccg
     1081 accgcgtgga ggcgcttctc ggctacaagg ggatcgcggc caggctccgc gaactcgagc
     1141 agcagatgtc ccgtgaagaa gcggctcttc acatcggcga cgatttcgtg gcaaagatgt
     1201 tcggggagac cacggccgag gagatcctcg accacgccat ccggggtgcg atggcgctcc
     1261 tgaccgaggg cgtggtggcc gccccgaccg aggggatcgc gaaggtgagc ctcgggaaga
     1321 acgatgacgg gaccgattac ttaaagatct attacgcagg ccccatcagg agcgccggcg
     1381 gcacggcgca ggcgctctcg gtgctggtgg gcgactacgt ccggcaggcg ctcgggatcg
     1441 gccgctatat tccccgcccc gaagaggtgg agcgctacat cgaggagatc cggcagtaca
     1501 acaacatcat gagcctgcag tatctcccga gcgagaagga gctccggacg atcatcacca
     1561 actgccccgt ctgcatcgac ggcgaaccga ccgagcagca ggaggtgagc ggctaccgga
     1621 acctggagcg ggtggagacg aacaccgtcc ggggcgggat ggcgctggtg gtcgcggaag
     1681 ggctggccct caaagccccc aaagtcttaa aaaacgtccg caagatgaag atggcgggct
     1741 gggactggat cgaggagatg atcagcggcg gcccgaagag cgacgacgat ccgagcgccg
     1801 cgatcaagcc gaaagacaag tatatccggg acctgatcgg cgggcggccg gtcttttcct
     1861 acccgatgcg caaaggcggg ttccggctgc gcctcggccg ggcgcggaac accgggtttg
     1921 cggccgccgg cttgaacccg gcgacgatgc acatcctcgg cgacttcctg gcggtcggga
     1981 cccagatgaa ggtcgagcgc ccggggaaag cggcggggat cgtgccggtg gactcgatcc
     2041 aggggccgac ggtcaaactc cggagcggag aggtgcgccg ggtggacgac gcagcggagg
     2101 cccgccggat cgccggccag gtggccgaga tcctggacga cggcgagatg ctggtcagtt
     2161 tcggggagtt catggagaac aaccacccgc tgatgccccc gagttactgc gaggagtggt
     2221 ggatgctgga agggggcagg cggcacccgg agagcgaact cgaggcgata gagttcgcgc
     2281 tcgacggggc tcccctccat cccgactaca cctacatgtg ggacgacctc cccccggccg
     2341 atatcgccct cctcgcggac cgggtcagcg ccgacggcag ggtcgaggac ggggtgctga
     2401 tgctgcccga cacccccgag gtgaaggcga tcctcgagga actcctggtc ccccaccgcc
     2461 ttgcagggga ccggatcgcg atcgccgaat acctggccct cctcgcctgc cttggcctga
     2521 cgctgcagct tgcgaagcgg tccacgtggc aggatgcacc catggagaac tcgcttggcc
     2581 tcgtgatgca cctctcgggc tttctggtcc gttcaagggc aggcacccgg atcggcgggc
     2641 ggatggggcg gccggggaag tcgaagccgc gggagatgaa gccgcccccc cactcgctct
     2701 tcccgatcgg ggacggggga ggttcccgcc gttcgttcca ggcggtctgc gcctcaaaac
     2761 accggtccaa cacggacggc ggggtgatcg aggtggaggt cggcgagcgg cactgccccg
     2821 cctgcggcgc cttcacctac aagaacctct gcgagtgcgg tgcgcacacg gtccgggtcc
     2881 tccggtgccc gaagtgcggg aaggatgtcg gggtggacgt ctgcccccgg tgcaacgtgg
     2941 cgaccgtctg cctccagaag gtcacgatca acgtcaaaac cgagtacgcg gcggcgctgg
     3001 agagactcgg ggtgcgtgac tcggcgatcc cgctcttgaa gggcgtgaaa gggctgatat
     3061 cccgcgagcg gccggtggag ccgatcgaga aggggatcct ccgggcgctg cagaaccttt
     3121 acgtcttcaa ggacgggact gtgcgctacg atatgatcga tctccccctg acccacttcc
     3181 gcccggacga ggtcggcgtc cccatcgaga ggctccggga actcggctac acccacgaca
     3241 cctacggccg ggagctcgtc tcggccgacc aggtgctgga actccgccac caggacatcc
     3301 tggtctcgga ggactgcggt gaatggctgg tccgggtggc gaagttcctc gacgaccttc
     3361 tggtgaggtt ctacgggctc gaaccgttct atagggccga aaaaccgctc gacctcgtgg
     3421 gccacctgct gatggggctt gcgccgcaca cgagcgccgg ggtcctcgcc aggctcatcg
     3481 ggttctcgaa ggccgcggtc ggttacggcc acccgttctt ccacgcggcg aaacggcgga
     3541 actgcttcca gggcgatacg gagatcaccg tcttttccgg ccggcagtgg gcgacgatgc
     3601 cgatccggca gttcgtgacg gagaatttcg atctcagcaa accggggctg gaccacgtgg
     3661 ggacgttcta ctccgacccc cggcagccgt tctacgtcag aagcatcgat tcccgcgggc
     3721 aggtcagcat aaagaaggtc acctccgtct cggtccaccg ggccccggcg cacctggtcc
     3781 ggttcgcgac gaagcggggg aaggtcctca ccgtcacccc cgaccacgcg atgctggtct
     3841 gggataccga ttacctgcgc aaagtccggg cgctcgaggt gaaggtcggc gaccgtctcc
     3901 cggcagagga gggcgggctc gtcatcagcg acgaggtcgt cgcccgggag accgtccagg
     3961 cgctcgacga ccgggtctac tgcctgacgg ttgcggagaa ccacacgctt gtcgcaaacg
     4021 gcatcttctg cgggcagtgc gacggcgacg aggactgcgt gatgctcctc ctcgacgggc
     4081 tgatcaactt ttcccgggct tacctccccg agacgcgggg cgggacgatg gacgcaccgc
     4141 tggtgctgac ttcccggatc gacccggcgg aggtcgacaa ggagagcctc aacgtcgatg
     4201 tctgcgacca ctaccccatc gaggtctaca acggctgcct cgcctacgcc aaccctaaag
     4261 acctcgacaa gttcgtcgac cgggtggagc ggcggctcgg gaccccggca caggtcgagg
     4321 ggttcctctt cacccacgcg accacgaaca tatccgcggg gcccctcgag tccacctaca
     4381 cgatactcgg gtcgatgctc gataaactcg aggcggagct cgacctggcg aagaagatcc
     4441 gggcggtgga tgccgacgac gttgcggagc gggtcttaaa cacccacttc atccgggacc
     4501 tccaggggaa ccttaacgcc ttctcaaagc agaaggtgcg gtgcatgaag tgcaacgcaa
     4561 aataccggcg gatgccgctt gccgggaaat gcacccgttg tgggggcaac gtcatcccga
     4621 cggtccacga gggctcggtg aagaagtacc tggagatgtc gcgcaacatc tgcgcgacct
     4681 acgcggtctc ggcgtacacg aagcagcggg tggaggtcct tttcatgcag atcgagtcga
     4741 ccttcggcca gcccccggag cggcagctcg ggctcgcgga cttcatgtga ggatgtgcga
     4801 tggcaacgct ctcgcctgct gaggtcctca cggcccttaa atcggggaaa ccggttctag
     4861 gcgactgccg gagtgcggcc gtcgagcagg tgagggacgg cgtataccgg gtgacgacgc
     4921 ccgcgtcggg gccaaaccgg accctcctct tcttccacgg cggggggttt gtcaccggct
     4981 cgacggccga cgaactcgac ctctgcgccc ggcttgcgga cgcatgccgg gccagggtcg
     5041 tctcggtcga ctaccggctc gcgccggagc accccttccc ggcggcggtg gaggactgcc
     5101 tcgccgccta ccgcgacctg ctcggagagg ggtgctcccc ggtgccggtc ggggtctcgg
     5161 ccggggggtc gctcgtcctc gcgatgctcc tctccgccct cgatgagggg ctcccgatgc
     5221 cggcagcggc ggtcgtcctc tccccggtgg tcgatatgct cttcctcggg gagtcggtga
     5281 tcttcaaccg ggagacggac tggctgacgc cggaacacct cgcagcgatg cgggaggact
     5341 acctcgccgg ccacgacccc gtcgacccgc tggcctcccc gctctacgcg gacctcgcgg
     5401 ggctgccgcc gctcctggtg caggcgggga gcggcgaggt gctgatcgac ggcatcacag
     5461 cgttcgcgag cacggcggaa ctccagggag tccccgtcac gttcgaccgc gtggagggga
     5521 tgttccactg ctggcaggcg tttgccgccg tcctccccga ggccgcggcg gcgctcgagc
     5581 gggtcggggt gttcgtccgg cggcaggttc ccgacgggcc gggggggtag ccgccatcaa
     5641 gctcattact ctgcacgttc aataggtaca ggcgcgaggg tagccaagcg gtcaaaggcg
     5701 gtcgactcaa gatcgactct tgaaggagtt cgcgggttcg aatccctccc ctcgcatttt
     5761 cggagtatat ctcttagcgc aatagttcat ctcttttgag ctttcacctg cacctgctac
     5821 ggtgaaacgg cactttatcc gatttttgcc aatcacttta ttcagacccc cattacttca
     5881 cttgcaccca tattcccagg cgatttaagg gccggcgacg ttcccggttt gtaccacaat
     5941 cccagatcag ggcatcttga agaatggcgc tgcaggatat tcaaaaaggg atagaaaacc
     6001 cttccacaga tccagatctg ttcgattggg gtcagaggtg tttttcggtt agtaattttg
     6061 cccacaagcg atgaggcact gctttccttc tatcgccagt tagttctgaa gcagatttga
     6121 acaccgaggg agggaaatat atacttaaaa tgctccttac ttccgcctat ggtcacaaaa
     6181 tgtgatattt gcggatatgc tattaatcca accgataact tttgtggagg atgcaacgtg
     6241 gatttaaggg aacctaagaa cccggatgaa cagaagacaa catctcagaa gaagaggtct
     6301 tcaaagggaa aggaacaatt atcaccgagg ccggacgcac caggtgccga cgtcatttac
     6361 tggtgcacaa ttcagtcatc atacacttcg tttgagatga cgttcccaaa attcctttat
     6421 ctggccgtcc tgttggcagg gggtacgaag aaattaggct tctgtcattg ttcgacatgc
     6481 aatcaaggat atcgcgagct gctagccgat ctcaccaatt ctacagagaa gaagaaatta
     6541 acagctaaca gccgggagat cgtaactaaa gcacgtgatg cgaaaatcga cggcgtgacc
     6601 ttcgatgtat ccaatttaat ttcaggttct aataaaaaag agagtgagca tcccattcat
     6661 gagatagacg gctctgtatc ctcgaaacca tcaacaggaa aaattacaat tgagatagag
     6721 ccggatgtgc ttgaaaatgc ggtcttcacc ctaacctccc gtagcgatta ggcgcacggg
     6781 accatctatt ctcacgatag tggctccttt caccattatt cgcttccttt ctccctttta
     6841 tgtccggctt actgaggcgt aatgtgccta taaaatttag gcactgcgat ccgcttggcc
     6901 tttcgattct aataggatta cagagatatt tccgaaaaaa agagatttat cnncttcacc
     6961 atattgatga gtgaaagagg gcgccggatc atctctgatg caaccagatc acccagaaca
     7021 gaaatgccaa agagatcctg atctagagat ctcagatcct ttcaagccac ctcgacgcac
     7081 ctggttattg taccagccag accagtaatc aggccgcaac ctcgccgcag atcctctcgg
     7141 cctgttccag gaccgttttc gtcgcctgtt cctgcaggtc gggaggatac ccgtacttcc
     7201 tgaggatcct cttgatcatc acccgcatct ttgcccggac gctctccttt gcggcccagt
     7261 ctatcgtcac actcttacgg acggctatga caagctccga ggcgatcttc tgcaggagtt
     7321 catcctgcat cacttcccgc gcactctcgt tctcggcgag cgcgtcatag aaggcaagct
     7381 cggcgtcgga aagacccagg tcatctccgc gtcgtacggt ctcacggaca tcatgggccg
     7441 tcttgataag ttcctcaatg atgatggcgg tctcgaccga acggttctgg tacttcttca
     7501 ccgatgcatc aagcatctcc gagaactttc tcgactggac catgttgcgc ctcagccggg
     7561 tccgtatttc gtcttcgatc agttttcgca gcagctcaac ggccacgttc ttctgcggga
     7621 gatcccggac ctcggcgagg aagtcgtcgg aaagaatgga gatatccggg ttcttcaatc
     7681 cgaccgcctg gaagatatcg atgatgcctt cgggggtgat ggcctcggag acgagctgct
     7741 ggacggcatg atcaagggcg gcccgggacc cggcggtccg tgtggttgtc tttgcgattg
     7801 aggctttgat ggtctggaag aacgccacat ctgtccgtat ccggtcggtc tcttcgtggg
     7861 gaacgcagag agagaacgcc gttgtgagct gcgtcacggc atcgaggaac cgcgacttcc
     7921 cgtcctgccg ggagaagaca tgctccatcg cggcaggaat gaacgagaga cgcttgccgg
     7981 gatcgcccgt gttccaggcc gaccagtcga aggtatggag catgtcgcag cagatctcgt
     8041 acttctcaag catgacgaag accgcctctt cctgctcctg gacgctcaga cccttgtgct
     8101 tcttcctctc gtcggtgaac tctgcgaggg cttctttgag tttgggcgca atccccagat
     8161 aatcgacgat aaggcctccg ggtttgtccc ggaagacccg gttcacccgc gcaatggtct
     8221 gcatgagcgt atgcccctgc atgggtttgt ccacgtacat cgtgtgcagg cagggggcgt
     8281 cgaacccggt gatccacatg tcccggacga tgacgatctt caagggatcg tcggggtcga
     8341 cgaagcggtt ccggatcttg ttccgttgcg ccttcgtccg gaagtgcatc tgccattccg
     8401 gcttttcgga tgcggaaccg gacatcacaa tcttgacggc gcccgacatg tcgtcgtcac
     8461 tgtgccaccc gggccggagg cgggtgacag catcgtacat ctcgacgcag atgcgcctgc
     8521 tcatgcagac gatcattgcc ttgccttcga gcgtctcaag gcgcttttcg aagtggccga
     8581 cgagatcccc ggcgatcacc tctatccggt gctctgagcc gataaccgcc tccagttgcg
     8641 cccacttcga tcgcaacctc tccctgacgc tctcctcctc gttctcggtg acttcctcga
     8701 aaccttcgtc gagcaccggt ttttcttcct cagcaagatc gagcttggcg tggcgcccct
     8761 catagtagat ggggacggtg acgccgtctt cgttcgcctg gacgacgtcg tagatgctga
     8821 tgtagtcccc gaagaccgcc cgggtatccc ggtctttgag cgagatcggt gttccggtga
     8881 aaccgatcga ggaggcgttg gggagggcct gccggatgta ttcggcaaac ccgtagcgga
     8941 tatatcccgt tgccttatcg acgtgtggtc tgaacccgta gtggctgcgg tgcgcctcgt
     9001 cggcgataaa gacgatgttg cgccgctccg aaaggatcgg atggctctcc tcttctttct
     9061 cggggaagaa cttctggacc gtagagaaga tcacgccgcc ggactcccgc tggagcagtt
     9121 cgcggaggtg taagcggctt tcggcctgga ccggggtctg ccggatatgc tcgctgcacc
     9181 gtgcaaaggt gtcgaagagc tggccgtcga ggtcgttctg gtcggtgagg atgacgaggg
     9241 tcgggttctc catcccgggc gcgaggatga tctttctcgc gtaaaagacc atggagaggc
     9301 tctttccgga gccctgcgta tgccagatga caccgccttt cctgtctcct tcgggggcgg
     9361 aggccgcaac ggtgctctcc accgctgccc tgacggcatg gaactggtgg taggcggcaa
     9421 tttttttgat gaacgttttt ccgtcgttct cgaagaggat gtagtcggtc aggtattcga
     9481 ggaatctttc tttctggaag agacctttga ggagaacttc gagttcgggg acgctccccg
     9541 gtgcggtctc gtcgccatcg atcgtccgcc agtgcatgaa ccggtcttcg tcggcagtca
     9601 gtgagccgac ccgggcctgg acgccgtcgg agacgatcag gacctcgttg gtgtagaaga
     9661 gggaggggat ctgctgctta taggtctgga gctggttgta ggccgaccag atggtggcgt
     9721 tctcgtcggc cgggttcttg agttcgataa cgcccacggg gatgccgtta gcaaagacga
     9781 cgatatcggg ccgccgggtg tggtcgccct ctttgatggt gtactgattg acgacgacga
     9841 ggtcatttcg tccggggtct tcgaggtcga tgaccttcgc gaggccgtgg cgaatcgctc
     9901 cattctcccg gtactcgacc ggaataccat cggtcaggta gcggtggaag gtatggttgt
     9961 tctcgatgag cgaggcggag gaggggacgg tgagtttgcg gacggcctca tcaacggtgc
    10021 tccccgggag ggcggggttg atccggcgga gcgcgtcgcg gaccctccgg aggaggaacg
    10081 gctggtggta gtcggtgcgc tcctcgcccg cttcgccggg ggcgatctcc ggcccggaga
    10141 ggacggtgta gccgaggccg gcgaaccacg caagggcagc ctcttcgact tccgattcgg
    10201 tgaatgtata gggggtcatt ctttcaaacc cctgaatatg aagatgtact ccttgcccga
    10261 tttctgcttc gccagatatc ccaatttttc gagataaaag agatcatttc tcgctgttcc
    10321 gtaagccaca cggtatgttt caacgatttc cttgatcgta atcggtttat caggcttttt
    10381 gataagagat ttaatgatct ctgcctgacg aagactcagt ccttcgctct tctggagaat
    10441 gtttagcgcc tgtctgcttt ctctctgctt cttctcgatg tatttctgga tattgtcgac
    10501 cgcggttttt actgccgcga gattgtagcg gatgaaatac gtgagatcgt tctcatcact
    10561 ctcggtatac tggtatgcta gtttatattg ccccttcgtg tcttttattg ctctggatac
    10621 tgccatatat tcgaaaagcc agtaatcctt ccgaagcatg taccagtaaa agatactccg
    10681 tgcagtccga ccgtttccgt cattgaacgg atgtatgtat cctaacagga aatgcaggat
    10741 gatgcccttg atcacggggt ggatgaattg ctcgccgtca ttatttgcaa atgcacagag
    10801 tttctcgatg aggtcttcaa ttaacgcgaa atcgggaggt gtgtgcagga cattgccccg
    10861 catatcaaag actctgatat cgttgttgtc ctcaactccc gtttaataag cgc
//
LOCUS       DAJB01000202            8244 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_83396, whole genome shotgun sequence.
ACCESSION   DAJB01000202 DAJB01000000
VERSION     DAJB01000202.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_83396"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1728 a         2366 c         2589 g         1561 t
ORIGIN      
        1 gaggacaaga atgagggtcg atgagttgtg gtagacgcgg ccgatgccgt atgtgacagc
       61 aaccgcgacg atcccgaagg cgaaggccga tacctggatg acccatgggt tgcccgagac
      121 gaggatgccg agggccgccc cgaacccggc gccagcggcc acgccaagga tatcgggtga
      181 gacaaggggg ttccggaaga gcccctggaa cgatgcgccg gcgagggaga gtcctccccc
      241 gacgagcatc gccgctatca accgcgggag cctgatctgg aaaacgaccg tctgttccga
      301 tgctccccag gtctgctcga aggaggggag gacgacctga tgaccggagg cggccgagag
      361 cagggacgac gcaccgtcga agaatgcgtt cgccgatccg atgacgagga tccggaaggt
      421 ctccaccggg tccatcatgt atctcccgaa gaagagggag aagaggaaga gggcgaccgg
      481 gaggaggtag atcgccttct ctgccgggat cagccggaga acggcttcat gggccggtat
      541 gccgtcccga aagaccgatc gccaaaaacc gatcgcaaca ttcttcctgg attcctgtcc
      601 gatctctata atcggttctt tcctatgggt catccggtat accgtccata tactcatctg
      661 tccatacttt tcatttaatt ttatgggaat gtaaatggca taacctttac aacaacaata
      721 aaaaggttcg gcggaccccg ccgtgttgca caatttcccg gggtttccct cacggatctt
      781 cctgcggtag tttcactcag gccaccgccg caaccagcgt gttgcaacag ttcacctgtg
      841 cctggtctga gatcaccgtg aggggcgggc agacaggttc ggggatagga atatatctcc
      901 agctaatgtg taaatatgtt ttatttaatt ttgctgaaaa gtaaatgatt atagataaat
      961 atgcgcgacg tcgcactatt gtacggtgta gttggtatga agaaagatgc tcgtcatctg
     1021 gttctcgcca ttgtggccgt ctgtgccgtc ctggctcttg ctttcatcct tgcggcgccc
     1081 gggacagccg gtgaagattc tgctgtaacc gtccggaaca ttactgatat ggacggcagg
     1141 acagtgacca tcccctccga ggtcaggacc gtggtcatca cctgctgtgg cggagcggcg
     1201 caggaactgg ctgccctggg agtcggcgac cgggtcgtcg cccagccgca acaatgcact
     1261 acggagcgcc tgtacaccgt cgtcccggga taccgcacca cacccgacgt cggttcattt
     1321 gatgaggtca acgtggagga gatcctgaaa ctcaaccccg acgttgttat cgggagttat
     1381 tatgcagaga ctggcaacgc ccgcattgaa gcggccgggg ttccggtcgt gcgggtactc
     1441 acggggcgtg ccggagtcga ggagctgaaa cgggagttcc tcatgctggg tcaggtcttc
     1501 gggcgcgagg agcgtgccga acggctggtt gcatactggg acgagaccct cgcgcatgtc
     1561 ggggcgaacc ttgacgatat ccccgagggc gagcggaagc gggtttatta cataaatggc
     1621 gcacccctca agacagacgg gcgcaatgcg tggggtgacg atatgatccg cgccgcagga
     1681 gggatcaacg tcgccggagg gatcgaccag aagagtggca gccagatctc tgccgaagag
     1741 ctcgtccggt gggaccccga ggtcatctac gttagttcaa acatgcggga ctactcgagc
     1801 ccggccggtg cgtttgcagc caacccccag tttgcgacca tcaaggcggt ccaggacggg
     1861 gcggtgtatc cctgccccgt aggaacgttc tggtgggatc gccccgcacc ggagagcccg
     1921 ctcgggatcc tctggcttgc ggagaaactc taccccgacc ggtttgccga cgtcgacctt
     1981 gcagacgaga caaagaagtt tttccgtgag ttctacgact acgacctcac ggacgaagag
     2041 gtagagagca tcctccaccc aacaccaatt tcaacggatt gatcaaacca tgacagaaca
     2101 tacaatccaa ctccggcagg ttatgccggc ggcagagcac gttgaacacc tggatgcgac
     2161 catatccggg tatcaggcct accgaatcct tgcggcagcg ctcgaacttg ggctctttga
     2221 ctggctctcg gagcgtggcc cgaccgcacg aaacgacgtc actgcaatcg gcataaacgg
     2281 gatgtttacc cggggttttc tcgcggcgct cgccgatatg ggatatatca cgttcgaaga
     2341 gaacgtgtgt gcaaacaccc caatcgccga agaactcctg gtcagcacca gccccaccta
     2401 ccagggcaac ctgatcctcg ccgcaggcgg ggagacttcg ccgtggtccg atctggccgc
     2461 aacgctcagg aacggcatgg agacaccgaa aaccggtccc cggccgacgc acgaccacct
     2521 cagggcggtc gcccagcggt gcacccgggg cgaactgcag cacgtagtca gccagatcgg
     2581 ccagcgcccc gagttcggga acgcccggtc catgcttgat gtcggcggcg gccacggcct
     2641 ctacgccgtc gccctctgcc aggagaaccc gaacctccag gcaaccgtct tcgatctacc
     2701 gcacgtggtg ccgctgaccc gggagtacat cggcaaatac ggtatgggtg ggaggatctc
     2761 cgcccgggga ggagacatcc tcgtcgacga tccgggcagg ggttacgacc tcatcgtcgt
     2821 ctcgcacctc ctctacaagt tccgggaccg gctgacggac gtcctcggca agatcacgga
     2881 atccctcgcc cccggtggtc ttctcgtcct caaccatctc ttctgctcac cgggctgcac
     2941 catatccccg gggagtggga ctgcagaact cgaccgggca ctcatgagcg ccggacatcc
     3001 gctctgccat ccgcagggcc tcgaggccgt gctgaagcgc ctgggataca ctggtatcac
     3061 cacctccccc cacgagaccg gaatgggcta tggcgtcctc ttctgtgcga cgaaaggtga
     3121 cagcgagacc gggcattgcc gggacggctc ctcgtcttcc gggtgcagtt gcgggccggg
     3181 cgggtgcggg aactgatggg caacagcaca gtcctcgacg agaccgtccg gatcctcaag
     3241 gagcgcctcg gggagaggat ggatgacgtg agggtcgagc gggcggttgt gggaatcttc
     3301 ttcaccggag tcaaactcag taccggccac gggggcatct gcgccacgcc cataaagtcg
     3361 atcccggaag ccgtctgctg cccgagttcg gcaaaggcga tgccgcactc ggggaggctc
     3421 cgggagcgga acataggggc ctacctggcc gacgcccttg gagaccggcc gatgcgcaag
     3481 gcgctcggga tcgcggtcgt cagcgcgtta tcggcgctct gtgacgatgt atgccccggg
     3541 ccggatcacc ccgttgtacg cggcgtcgat gcggtcgatc tcctcacgat acaggagagc
     3601 gggaatgtcg tggtcgtcgg ggcgctgacc ccgtatctgc gtgccctcaa gcaggccggc
     3661 cagccgtttc gcgtcctcga gatggatccc cgtacgctca agcccgacga gctgccgttt
     3721 tacacgccgg tcgaggagac ggatatggtc gttccctggg ccgacaccct ggtcatcacc
     3781 ggcaccacgc tgatcaacgg gacgctcgac cacctgctcg aactcgcccg gccggaagcc
     3841 acggtggtcg tgctcggccc ttcggtgagc atcctccccg acgctctctt ctcgcgcggc
     3901 gtggagatcg tcggtggaaa ccaggtgacc gaccccgatc gcctgctgga tatccttgcc
     3961 gagggcggtt ccggctacca cttcttcggg aagggggccg accggatcgc cgtccggcgg
     4021 tcccgggcac cgtgaacgca ctaagggaaa aaaagggcat ggccgcctta gaacgagagg
     4081 aaacggggtt tcctcttcca cggtgcacgc gtgttcgtgt tcctctcgga cactatcaca
     4141 ccatgccctg tggatgggct accggcaaac tgccggtaat ttcttaaatg cttcaatcca
     4201 gctggagaac cgttcgatgg cattgcgctt cttgtagagt tctggatcga accagaccgg
     4261 cctccttcgt ctcggaggtt ttcgggctct ctggttgacc cggatattgc tccggatctc
     4321 tcgtttccgg ctgcactgac gaatctcctg ggcaccatag gttgcatctg ctgagatgat
     4381 cgttggttgt tcctctgttc cgggaatcca aaatgctttt agtgtccccc tgccggggtt
     4441 gtgacaattt ttcacgcccc atgtaaacta atatatctta actttttgat attgttaaac
     4501 ataaatatta tattgccgct ctgtagtaca tctaatgttc aaccgtcggc ggtatgggac
     4561 gctgatgtgt ggcgggcgtc ctccgttgag gagaagaaag agctcttcac ccattggggt
     4621 tcaggcggag tatttcgatc atacggatgt gtgaaacaaa atttggatgt ggtagcatgt
     4681 gtgaatcaac cagtgagccg gtatttgccg gaattgatgt cggatcgctc acgacgaagg
     4741 cggtgatcat atcaaacggc ggcatcgccg gaagcggtgt gatgcctacc ggcgtccggc
     4801 cggaagagag cggcccggac gtctttcgca tcgcgctgaa agccgcgaac ctctcagagg
     4861 aggacgttgc ctactcggtt gccacgggct acgggagggt gtccgccgac tatgcagata
     4921 agacggtgac ggagatcacg tgccatgcgc ggggcgcata ctacctccac ccggctaccc
     4981 ggacgatcat cgacatgggc ggccaggact gcaaggtgat acgcgtcaac ggcgagggaa
     5041 acgtgaccga ctttgctatg aacgacaagt gcgcagccgg caccggccgg ttcctggagg
     5101 tgatgtccgg cgtcttcaag gtcgacatcg gcgaactcgg tccaatggcg ctccgggcga
     5161 cggatgtcgt gccgatcagc agtacctgca ccgttttcgc agagtctgag gtgatctcgc
     5221 ttattgcacg gggagagcca cctgaaaaca tcatcagcgg gatacatctg gccatagcga
     5281 accggatcgc ggggatggcc gcccgcgtag gcgtcgaggg cgacgtcttc ttctccgggg
     5341 gcgttgcgaa gaacgcaggc atgaaagccg cactcgagcg ttctctccgg tcgagcatta
     5401 ccgtaccggc gttcgacccc cagctcgtcg gggcgctggg ggctgcagtc attgcagaaa
     5461 caaccgggcg gaaggcaggc gcatgacgcc cagagcaata acgaatatcc gggacgccct
     5521 cgcgtcgaga ccggcagaga tcgaggctgc ccggaagcag ggtgcaaagg tgatcggctg
     5581 gttcgggtat accatgccca tagagatcat ccacgcgctg gggctcatcc cggtacggct
     5641 cgggaggggc ggcgacgacc ggctggtgga gatcggggca cggtacatct ccaaccagaa
     5701 ctgcgtcttc ttacgggcga gcgtcggtct ttttgccgag aagaacgacg tctatgtgaa
     5761 gaacgtcgat gcggtcgcta tcgatggcac ctgcctgcag atgtaccgta tggcagacct
     5821 tctggactac tattttaagg tgaaggcggt cgtgctctgc gtaccaagaa cgtttcagac
     5881 gactggggcg cgggagtatt tccaccgaga actccagtgg ttcgtccacg aacttgagga
     5941 gattgccggc accccgctcg accccgagag gctcgccggg tctgttgcgc tctactcgtc
     6001 catacgagac acgacccgga aactctatcg tcatgtctcg cggggccgat catccctcac
     6061 ctggagggaa ctctacgagg cggtccacgc cgggtattac ctaagcccgg agaggtatgc
     6121 gtcgctgtta cacgaactgc ttgccggggt taagcacgga gagaatacga tggcaacatc
     6181 ggacagcccg cgaatcatgc tctcgggcag cgtccttgcc ccgggagaca cgaaactgat
     6241 cgagatcatc gagcaggccg gaggccgcgt ggtggtcgat gacctctggt cggggttgag
     6301 cccgtcgctg gtgcggatca acgaaccgac cgtcaccggc atcgccgacg cgtacatgga
     6361 cagggttcct catgcagcgc aaccgcatct tgaccgacag accgatcatc ggctggcgaa
     6421 cctgacgagg ctcgcgcggg agagcaacgt agatggtgtg atctaccata cgttgcggta
     6481 ctgcgacccg ttcacgttca aagcgattga agtcaaggag gtactgggca acgagggcat
     6541 acccatgata gagattcata ccgagtatgc cgggtcggat accgaggcta tccggacgag
     6601 ggtggaggcg tttatcgaac tcctcacgaa caggggatgc cggcccgggc agcctgtggc
     6661 ggaggtgagg gtgtgagcgg cccggtatgc acgccgccct gcggcggtac tgggcagggc
     6721 atgcggcaca gcgatcaggg gacgttctgg taccccgaag aagttcgccc gctcttttta
     6781 gagactcccg accttgtctt caaggatggg agacgagtct cgccggcgga gatctgggac
     6841 tacatgacag aagaagcgcc aaaacgatat ccctatgcct acagcaccaa tccctcgttc
     6901 ggtggccggt tttcctgcga cgtatggctc ctcagtggta taaagcgcag ttatatgggg
     6961 atgaccgcga tagaccggct caaggacgcc cacagcaaag gtaccccgct tgtggtcatc
     7021 cagggaggct acacgttcga gccgtactat gccgccggct gtatcccggt aggccccatg
     7081 ttcccgagag gatggatcat gaacctcaag gagggcaggg acttcgacga atccaaccgg
     7141 atggggatgg ctctgctgga agagtcacga aagatgctct cgatagagtc atgcaacctg
     7201 atcgcgtgcc tgacaatcat gaagaagatg aaggtgcccg tcaacatggt cgctccctgc
     7261 ctgtgtaccc ggtgttcgga tatggcgtac gtgaccgagg cctgccggca tggcactgtt
     7321 agtcaggctc ctctgcacat tgtggactat ccggtcaacc atcactgggg ggagtggcgg
     7381 gtcgactacc tcaagggaga actgcaaacg cttaccgaga aactcgggaa gttatccggc
     7441 aaggaggtga cggaccagtg cctgagagac gagatacggc agcagaacta tgcccgcagc
     7501 ctggtcaggg agtgccagaa gacgtggtgg caggcgaagg tgccaccgac aaacagcagc
     7561 gatagcggga tggcacacct gggcgtcggc ggcatgttcg actttaccgc ggccacgcag
     7621 atcctggaag agacgctgga tgagatacga acgcgggcaa agcagggtgt caaaggcgca
     7681 gggcttgtgg acgacccggc caggctgtat atctgcgggt cctgtgcaca cacaaacccg
     7741 ggattcgtgg acagtaaagg cggcgtcatc gtcggaaggg acgatatgtg gagtacagcc
     7801 accatggagg tacaggaggc gggcgacccc tacgagaacc tcgcaagggc gatggcctcg
     7861 ctgccctacg aacggcccac ggtggagcgg gcggaatgga ccgtcgagca gattcaggag
     7921 tcccgcgctg acggcgtgat attcatccac aactggggct gcaactacca gagcgcgata
     7981 gcccgcatgg tcaccgatat cataaaggaa cggacagggt tgcctgctat cagcatcgag
     8041 gtcggagaac tggcgaggat ggagtcgacc gagcagttcc agaaccgcgc cgaggcgttc
     8101 attgagatgc tcgcctgaca gaattgcttt tcccagaatc tggagggatg gaattggata
     8161 ataaaattag taacaggtca ggtatacctg taagccgcag ttggcatgaa gggttctccc
     8221 tcgaatgctc tccggatggc cgtg
//
LOCUS       DAJB01000203            5398 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_10069, whole genome shotgun sequence.
ACCESSION   DAJB01000203 DAJB01000000
VERSION     DAJB01000203.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_10069"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1197 a         1599 c         1614 g          988 t
ORIGIN      
        1 ttccatcctg agtgctgcaa gagactctta ccatctacca tacccccaat attttcgagg
       61 atctccctga atcccattga aaaacattct tgagacaggg gggcaatcta tatatccgat
      121 aatggatatg catgatgcag aacccaatgg agagcgctga tcaaaatgga tggaaaccta
      181 cagatgaaac agaagatcag ctccgctatc tcttctggcg atctgcgtga gcttgaaaag
      241 gtagtttcgg acatatcgga gacccagacg aggaaggaga agaaatcact ttacgagcag
      301 atgaatgccg ccctggtcga agcggcgttc gacgaagccc agcccgaact cttaagccag
      361 ctcgtcgaac cgaccaggga agagatattc gccctcgcca ggcgtgcggc cacgctctat
      421 agtgagaaca aggatgaagc atggttctct gcaatattta ccctggtgga caaactcgac
      481 cgtaagagcc accagtccga tatcctcgcc gggatctccc gcgatctggt ccaggccggg
      541 gtcgataccg gcgacatcca ctatattgag agaggaggag aagccttcga taagatcagc
      601 atccgtaaat accgctcggc aatcctctcc gagatcatcc cgctcttcat tcaatacgga
      661 cagaaacacc acaacgtcga catcatgcag tatgccctcc agatgctccc cgagatcggc
      721 gacatatcaa gacaatcaca actccacgcc gacgttgccc gggcgatcgc cggctccggc
      781 attgagtcgg gtaatatcag cctggtgatc agcggacttt cgagcgctac cgagatcaac
      841 cagaagatcc ggcgcaccaa ctcgatcgcc gacatcgtgg atgccgcctg gaaatcctcg
      901 ctgaagaagg agatctccga tgtcgagcag atcatagact ccctcccgga tctccccgaa
      961 gagcggctca ccgaggtcct cgcgatcctg accgagcagc tccttgaccg gcagcgggac
     1021 aaaaaacagg tatacaccaa actgctcagg atcgacgacg aaaaactgtg ggcgggccag
     1081 acccttgtcc ttgaactctt gaaaaaggca gagcggagtg gcgaacgctg gttcctcgag
     1141 aaggcgttcg agttcaatgc ccggggcgtg ggagagaccc aactccccat cgaggagatc
     1201 gtcctctccg ggattgcagt cgtcgagaag agcggtaacc cgaccgttct cctcgacatt
     1261 acaccgttga tcgacgagtc ctgtgatgcc gcgaaggcgg cccagctcta ccggcagatc
     1321 accgacgcac tctcccggat gggcgacttc taccacgcga tcgaggttat gaagaaggct
     1381 agcgccagca cgcagaggat caacgcccaa tttttcgata ccagtgtccg cctgatcaag
     1441 gagggtgtcc ggcgtgacga gatcaaactc gtccgtgaga acatcctcgc cacactggat
     1501 atcgatattg ccgactcgct ggtacaccag gcggtgaccg acttctgcaa ggagtacgac
     1561 tttgacgaag tcgtcaggca tataccggcc ataaaggagc tcgcaggatc gcaccgtgca
     1621 caggaccccc tgctccttga atgcaacacc atcctgatcg agcgggggtt cgtccgccag
     1681 aacgacccgt cggccctcgt ggatctggtg agccagatcg aagagcaggc cctgcgcgag
     1741 caggcaatct cgaccatcgc cgtcgagatg gcacggatcg gcgtctcccg gaaagaccgg
     1801 gacctcctcc ggcatgcgac cgccctcacc tgcgaaatca tggatcagca ggcacgggcc
     1861 gaggcgatgg gcggcatcgt ggaccaggcg gccgtgcttg ccgtcgagga cggcgacctc
     1921 gacctgctcc acgggatgcg ggtcctctcc acatccctgc tggagcggga ctactgcctc
     1981 ttcgcgatgg gcaaggttat ccacggcctg atcatgtacg ggatcgagca gctctcgctc
     2041 cgggcgctgg atgaggcagc ccagatcctc tcccagatcc gcgacccgtc cctgcagcgg
     2101 cagctggccg accccctcat cgagggctac gtccgggtcg ggagcctgca ggcggcagac
     2161 cagctctccc gggggaaggc ccggatcttc gacggcatga tggaaccatt cgagatcgcg
     2221 ctcaccctcc tccagaccag cgccccgcgc gaggagatat cgatcaagat cgcgagttac
     2281 gtcgatatca tgctcgagta cacgcaggtc tatagaagcc cgatctttgc ggtccccatg
     2341 acgctcttct ccctggagat cgagggggat tacgaacgga ccgccatgat ccagaggatt
     2401 ctcaccttct tcaccagtta cacaaaggag ttcgactcgg ccgatcctta tgaggtgacg
     2461 gcttacctgc tcgaggggat cgggggggga gcgacgacac agccggtcct cgagttgatg
     2521 taccgcctct tcgagcacac cggcgacgtc tatgcgcgct actccgggat gtaccggatc
     2581 gtgagcgcct attcggtcct cggcaacgtt gagcgggccg agatgatcat caggaggctc
     2641 catgagacca tcggcaccat ctccgagccg cccatccgtg cgatcatgct ctccgacctc
     2701 gccggactca tggccgggat cgaccacagc acagccagag gctaccttgc cgaggcgcag
     2761 gagacgcttg agttcgtggg gcaggaacac gaagcattcg tcaggaagaa cctgatctac
     2821 gccgcaagaa gcctcagcgc cgtaagccac caggagaagg atgtcgagtg ggcgatcggg
     2881 caggtcagca ggatcgagga tccggtcgag tacgtggatg ctctcgcggc ggtctttgac
     2941 atggtcaccg aacctgcaca gcgaaaggat atcctctcgg caatgtgcca caccgtcgtg
     3001 agcattcctt ctccctatgt gcggctctcg atgctctttg acgtggcgca gttcgccgaa
     3061 tcctacggtg acgaaaagga gattgatgaa ctgctggagg gtatggagag aaccgccggc
     3121 tacgttcaga tcccgttcat caccgccatg acgcaacagc ggatggccca gatgctcttc
     3181 tcgttctacc gggcgagtgg gaagccggcc gcccagcagc gggctgccga tatcgtctcc
     3241 accatcgacg atgaccggat ccggtacacc ctgatggtcc agctcgagca ggccatgccc
     3301 cagtcatgga agaacacggt ctacggcagg atcctcgact gccgggacaa gatccggagc
     3361 ggcgattaca ccacaaaaga catggtgaca ctcgaccggg cgatacgggc ggcaccggac
     3421 cgggcaaaac gggcgactta ctacaccgag ctctacctca ttgccaggaa cgcaggacag
     3481 catgaggttg cggacaggat gctcctctgt gcgctcgaag aggcacggat catcaggccg
     3541 ctctcgcggc gggcctttgt gctcggtgat atggcgtgcc ggatctacgc agagcgctat
     3601 gaagaccgct caagggaggt cctcgacatg gctgtgagcg aagccctcaa catccgcgac
     3661 tcgacgctcc gggacgaagt ctacgacgag ctggatatgt ccatgcggat catccaggag
     3721 cactggctgt gaagacgatc gagatcagga tctcaggaag ggtgcagggt gtcgggtttc
     3781 gtgcgtgcat caagaggatc gccaccaacc tcggcgtcgg cggcgaggcg atgaacctcc
     3841 ctgacgggag ggtgctcatc accgcaaccg ccgaacccgt catcctcgac aagttcgtct
     3901 ccatgcttta cgggtgcccc cgggtcgtcg tcagggatct tatccagcag gagatccccc
     3961 ctgccgccta ccccgagttc accatccagc ggggcagtta ccagtacagc acgtgaaatt
     4021 cggcgttcgt gaggagcgca tcccgcagga tgcgcacctg agcatcgata cgcgactggt
     4081 cgatggagtc gcggagcgcc gcgaccagcc gggccgagag gttcccatca agatagcatc
     4141 tcttttcgcc gcattcatag tccccgtccg agacctcgtc ccgggcaaac cggaggacgg
     4201 cacggtccac catcgtcgct ttctgcggtt cgatgaggtc gtggacgaag ctcccggccc
     4261 cctcgtgaag catgccgagg tcggggtcga ggtgggcacc gatcattgat acgcaacagt
     4321 tgccgtagag catcgcgtat ccgagcgaga acatggcatt gacggggtcg aggtaagggc
     4381 ggctcgtcct gcgccggaac ccgagtcccg gcggaagtgt gcgggagagg atctcatagt
     4441 acatatcagt ggtcaggcgg gagagcctcc ggagctcctc catagtgacc gcgaccgcga
     4501 gttcctccct ggcctggtgg aggaaatcaa gctctccggc gtagaagata tcctgtccgg
     4561 cgcggtcgta gagttcctca aggaggagaa gcctcgactg gaggccggcc gttgccagcg
     4621 gctgcgcaaa ccggtggggg gcggcctgtt cctgggcaag gcgcaccgcc tcgtccggcc
     4681 ggtagccgta gggatacagg tatccgacgg gcgtgccgtc gatatcgaag aaggtgatgg
     4741 cagcaccggc tttgaggagg tttgtcaccg ccgaggtgtg cagggtatgg ccgccgacga
     4801 ccaggaggtg cttcaccgcc tggagggggt agcgccgggt ggcgctcccg tgcgcgatga
     4861 ccagttcctg tgtcgtcgct ttgatgtgcc ccccgtagcc gaagacgggg agccagggtt
     4921 ccgtcgctgt catccaatca ctctggaaat gaatataggg cggctttcgg tgattaatgc
     4981 atcgactcgc cggatccagg gagatcctcc ctgtgcagga caggtgggcg aggatccaga
     5041 tccagtcaat atagttcaat taacttaagc gagttaatta actgcaacgt ttttacacca
     5101 ccaaatccca gtttaaaatg ttatttctcc agaaaaggct ctgggaaaga ttatgaacta
     5161 aaatgggttg atttgatgaa cgaaacgagg aaaacagttc agtacatcct gtttgtactg
     5221 atggtatgct cgctgctggt actgccggca gcaggggaga cgctccccgg cgggaacgtg
     5281 agcccggggg agagcaccgt gaataccaca gtgacccctg aagtgactga gacagagacg
     5341 ccaacggtga taccgacaga cgagccaggt atgagattcg ccgggaccct gtctgagg
//
LOCUS       DAJB01000204            5092 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_130121, whole genome shotgun sequence.
ACCESSION   DAJB01000204 DAJB01000000
VERSION     DAJB01000204.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_130121"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    558..672
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4109..4206
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          969 a         1493 c         1471 g          946 t
ORIGIN      
        1 acataccggg gcgtcaacga ctatctttct acgaagtatc ccgcctcgtt cgccgatgag
       61 cgcgactact ggctccagtt taccgacgag agcatccagg accggtacct ccggcagtta
      121 gccgacaaca tccgggctgc cgagaccgac cgcgaggatc aggcgcgggc cgcgatcagc
      181 atggtgcaga agatccccta catcggttac tcgtacgata cggcggccaa gtacccgtac
      241 catgtgctct accaccagaa cggggactgt gacgagaagt cgctcttgct cgcgtacctc
      301 ctccgggacc tggggtacgg tgtcgcggtc tttgaattcg agcaggaaag tcatatggcc
      361 gtgggcgtca gggcgcccga gcggtactgc tacgggaaga ccgggtacgc gttcatcgag
      421 accaccgtac cgaccatccc gaccgatgcc ggaggggagt atggagaggc cgaagtaaag
      481 ttgaagtcag atccgaaagt cttcgtcgta gccgacggag actcgtttga gggaatctgg
      541 cgggagcata ccgacgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnacggcgtc ctcgtgatgg tcgccgtcac catcatcctc gccgctctcg
      721 tcctgctgat gctgcttgcg atgattcctt cctggtcgtg ggaggagccc gcacagcccc
      781 cgatcatcat gaccgggatt acccacacca gtcaggggac agggctgctt acctgtgcaa
      841 gcagggtcac cctcaagaac aacggcagtg cggtgtacga gaacgattgc ctgaaagcag
      901 tcttctacgt aaacggccag aaagtctgca tcgtctatac cctgaacggt tacctcctca
      961 tcccgtcgca ccactacggg gtgaagactc tcggcggcga gggctgccgc agcccctact
     1021 ggaacccggg cgagacgatg gtgctggatc tcaccgataa caccctctac ccgggagcac
     1081 aggtcaccat ggagattctg gacaaacgca ccgggatggt gatctcgaag cacacggtga
     1141 gggcatgagg ccggccggca ccaaagacca tgtttttgta tcgggacgct tctagagact
     1201 gtatgttcag atcgatactc gtggcagtcg acggatcgga gataggccac cgggcgctcg
     1261 aagaggccct ggatatcgcc cgtgccatgc aggcccccgt gcacgccatt catgtcgtcc
     1321 agaccgggac gtatcccacg ctgatcctca gcgatctcga accgccggat ctcacccagc
     1381 aggtgctcct cgattcgctc gaccgggagg cgaatgagat cctcgccgac gcggaacgga
     1441 aggctgccgc cgccggtgtc cggatgacca tctacaagcg gtgggggcac ccgggagcgg
     1501 agatcactgc cctcgcacag gaactcggcg ccgacctgac cgtggtcggg tcgcacggca
     1561 ggggccggat cgaccgcttc ttcctcggga gcgtcagttc ctacgtcgtc gatcacgcgg
     1621 cgtcaacggt catggtcgtc aggggctgac gacctcacaa cgccccgggc gacgagccgc
     1681 gccaccctga ccggctcggg cacccttcct tcacgggtga agtcgttgca gagcctcgca
     1741 gcatccgcgg gagagaggcc gtaggggcgg atgaacaatg tgtatccgga atgcagcaaa
     1801 accgggatcc ggtcgccgag ccgccggtat gccgcgagcc tgccggaatc gtcggggaag
     1861 tgacggcgga tatcctcctc aagcccctcc gactcctcgt aggtggtgac gatgacggga
     1921 agaccggtcg cgtcctgcac cgccaccggg tcgatgatgt tgtaccaggc gatgacgctc
     1981 ccgctgagca tcaggaggtt gatgtcccgg cgggcgagat cggtgaagac ccggatgact
     2041 gcatcggttg catcgcaccc gcccacagtc acccgggcga aggccacccc gtcgattaag
     2101 agatcctttc gcatgacgac gccggcaagg gtggattgct cccgccccga ataactctcg
     2161 gcgataccga gggcgcggag ccccgatttg gctacgtgca tgcgaagccc ttatgatact
     2221 aaattggata tagtacaccg atgaacatcg agcgagatga agtctgcatc ttcatcccca
     2281 cgttaaacga ggccccgacg atcggtgagt tgatcgaggg gttccggcag cacggttttc
     2341 tccacatctt cgtcatggac ggaaacagca ccgatggaac acccgatatc gcacgatccg
     2401 caggagcggt tgtccggacg caggcgggga agggaaaggg caacgccatc atcgaggccg
     2461 tggagctgat cgatgagcct tacgttctca tgctcgacgg cgacggcact tactcgcccc
     2521 aggacgcgga gaggatgctc gaccccctcg ccctcgggtt cgaccacgtc atcggcgacc
     2581 gcctcgcgaa ccctgagccg ggggcgttca cccgcctgaa cctcctcggc aaccagctcc
     2641 tcaacgtgat gtttcgaata gcgcacggaa aagacctctg tgacatcctc tccggctacc
     2701 gtgcctttac ccttcgctcg gtccggcaga tgaccctcaa agaggcgggg ttcgagatcg
     2761 agacggagat ggcggtcgag gcggtgagga acggacaacg ggtcacggtc gtcccggtcc
     2821 ggtacctcgc gaggcccgga accatcacca aattaaaccc cctccaggat ggcttcagga
     2881 tcttttcgac catataccgg ctcgcaaaga tgaacaaccc gatcttttac ttcgggatca
     2941 tcggcctctt catatcgctt gccggtggcg tcatcggcct ctatgtcatc cttgaatggc
     3001 tcaggaacat cgagcacctg ccgctcacca tcctcacggt ccttctgata acgataggct
     3061 ttcagatctt catgttcggg gtgatcagcg acatgctgct tggattccac cgcgagacca
     3121 cccgccagat cgagcagtta cagacccctc ccaagtcgcc ccgataggaa gaggatccgg
     3181 caggcgtact cggcgatcga ggtgtagaga tacgcctcat ccatcgtttt tcctgcggca
     3241 aacgtcccgt gtccccggac gatgacgata tgccctcccc ggagggcccc ggcaacgttc
     3301 tctgcgatct cgtcggtccc ggggtttcct gtaacgaccg ggatctccgg gcagagtatc
     3361 cccccttcgc tgtcggccgg ccggaggagg tcggcgtcga gcgaggcggc gacggcgtgg
     3421 acggggtggg cgtggacgat cgcacgatga ggcgtctccc ggtagacggc ccggtggacc
     3481 cggtactcgc ttgatgcttc gcgcggtgca tcgcccgtct ccggcacaaa gaccggcatc
     3541 tcccccgcgt cgagataggc gccggtccgg gtgatgtgga accccccctc ggtgcgcagg
     3601 ctcatgtttc cgaaattcgc tccgaccagc ccttctagga agagccgctt tccgatccgc
     3661 tcaaattcct gatccagcat tcattacacc ctttcgtaca gaatgctttg gcgtaccccg
     3721 ggatatgtgc atgccgcttg gggcgtgagg gcggggttca agagtgctgc aggggatgcc
     3781 agcggtatgg gtttggagag cctggtttga gtcagaggaa ccacagatat gcctgatggg
     3841 tgttttcttg gcaagcccgg tccactggac cgtggtgata tcgccacgca ctgcccctgc
     3901 ccgggcaaaa aaaagaaaat tatacgaatt tcacgccgac gtccttcatc ctgttgaacc
     3961 gggcgatgtt gaggaggagg ggtgtgatgc tctcgaccat ggatgcggcc gcaagcggtc
     4021 ctgcgtcgta ggcacggaga ctcgatacgt tgttgacgat ctccatgacc tgttgcttcg
     4081 cactgtcatc atcgcagcag acggcgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnagta gaagtaggtc gtccgctcga gcgggttgac ggggctgacg acgatcttgc
     4261 cctcaaaccc ggtcagggtc tttaaggtgg gagccacgtg cttgaacggt atcgcgagga
     4321 cgacgatatc tccctcgtcg accgcacgct ggttggagac gccgagcagg ctgcatgcga
     4381 ggtcccgctc ctgcagggtc tcccggcagg tgtcgcaggt tgcgatagcc ttcgtctcct
     4441 cgcgggatcc cacgatcacg tcgtacttcg gagagagccg gagcgccatg ccctccccga
     4501 tgtcgccggt tccgccgacg atgccgatct tcactgctcc accagcggct ttaggatgct
     4561 ctcaagcgta ccgaggtcct ggacgcccac cagtttctga accagattcc cgtctttctc
     4621 gatgacgagc gtcggtacga attggatgtc gtatttcgcg gcatactgcc gcgtctgttc
     4681 tgaatcgaca ccgacatcga tctttttgat ctcgacctgg tcgcccattt tcgctttgag
     4741 ctcatcgatg atcggtgtct gctggtggca cggaccgcac cactcggcaa agaagtccat
     4801 taaaaccggt tttcccataa aactataccc cgttcaaaca ttgtgtgtga acggggtaat
     4861 aaagattatt gtagggttgt cattttgaga ggatcgccat gataatcttt ccgtacgcgg
     4921 gcctggtgac caggacaccg atcagaacac cgagtatggt gatgatggcg aaccctctga
     4981 gggtggagag gtccatcagc gccagcggga gcattgcgac gaagaccgtc gctgcagcca
     5041 cggcgatgat cccgaacgcc cgcccgtacc gcttcatgta gaggttcggc ga
//
LOCUS       DAJB01000205            3072 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_174507, whole genome shotgun sequence.
ACCESSION   DAJB01000205 DAJB01000000
VERSION     DAJB01000205.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_174507"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          668 a          891 c          911 g          602 t
ORIGIN      
        1 ctcaattccc atctcccttg cccgcgtatc aagcatcgcg tacaccgctt ctatcgagga
       61 gacgttctcc ggcagaggcg ggccgttcca gatacccacc cggagatacc cgtcgacgtc
      121 gtagccgaaa ccgatgagag acccgttggg atagaggtag cgttcccgta tcagattctc
      181 tgtggcggtg gtgaagtccg gaagcgatgt gctttttccg cccttcgaga agggaagccc
      241 tccgagcgtg acggtaatgg gctccctctt gttcaggctg gctttgaggt gttcgatgtc
      301 tggttcggta aatactggtt gagaggattg gagtttcgcg gcagatgttg ccgacccctg
      361 atcccgtagg ccttccggat aatcagaacc gttaatcccg ttaaagtggt gctgatcgat
      421 tccaggcccg ggatctgccc gtacagaagc ggattgtgaa gaaactcccg aagcaatcct
      481 atctgcaaca gagcgatatt tatgtaactg tctaagcctt tctgcaagtt ttctgctctc
      541 actttcactc atgtcgtcaa gatatgatat ctcgggcatc tcttcgatca tttgagaaat
      601 ttccctgtcg ctcaatctgt ttcctgcatc atcaaagaga tgaggacttc gctctagcac
      661 tacattcctg tctactggat cccctccgga cggacggata ttctccggag tgtcctcatt
      721 cgcgctcacc gccgccacga agatcgtgcc gacgatcagc aacgccgcaa gcagggcgct
      781 gatcccggag aggaatttcg tattcatggt tccagtcctc ctttcacgcg catcccggga
      841 ggccacggaa ccgcaacggc tatctccgat gcgcagccaa cacgaccatg agcctccgca
      901 ccgttgcagg cgccgcctgc gaaacccatg gttgagaact caacgttctt ctatatccct
      961 tttctgtcac gatcatctac acatcgggcc tctccgatcg gtttcgctcc ccggtcaggg
     1021 tgccattggc cgaaaccgga tttgcgctgc ggttcgaaga tcggtgttcg cttcgacggc
     1081 catacctctg aataggcccc gtgttcggag actgccggga tgaccgcaaa atcatcggat
     1141 cgggcttgaa cccccggcag gaggggccgg aggaaccagg gtgggcgccc tccccaggcc
     1201 ggtcgctggt tgttaagcct cacaatctct ctcccggggc tatcgcgtat cgtgctcatg
     1261 cggagttttt gcggatgata tatattctcc gtacggagta cccgaccacg aagccgggta
     1321 tgagcgtcat aggcactgca atgatgagcc accagaaaaa atccacgctt gccggcgccg
     1381 acagccagaa taggaagaaa aatccggtga ggactccgaa gaacgctgag accgggagga
     1441 aaaaattccg atggcaagcc aggcgtactc ctccatgctg agttttgcgc cgctgctgcc
     1501 gaaaatcttc ggccagaaga accagacaag gtagacggca gcgcagatac ctgttacggt
     1561 gagaaacgac tcgaacatat gaactccact accctgttaa gcaacgacgt taatcgagat
     1621 ccccggctcc cgcgggatac cgcatgcggt atcgtcaatc gagacatcgt tgttcgtctt
     1681 catacagttt ctgtgccaat catcgacacg tcagagcatt cgaaggctcc gaaccgggaa
     1741 caagatccgt tctgcagata atccggtact cgccctggat gccggcggcg aacggagtgg
     1801 gcgggctgac cgcccggccg tcaccggatg tccatccgca tgaaacgagc gtatgccgct
     1861 ccgaagaaga ccgccggaaa cgcaagcatg gtgacgatcg gctgccagat gagagcgatc
     1921 aggccgggaa gatcgggaag cgggtcctcc atggggttgg gcgtcctcaa ggcgaagggg
     1981 tcggggatga tccacatcga aacccgtgga ttggtgatcg ccagcgagag ttccaggtaa
     2041 ttcctttgcg gcgagagcag atcgacggcc attgcgatac ggttccggtg cccctcgaag
     2101 gatcgctgct cctcccggta ctgcagccac tcctcctcgg tgaaatgcgg gtcgctcgta
     2161 tccaccggct ccgggggcgt tccggcagtc gcatcggtaa ggatgccacc gaccatcggt
     2221 acgacggagg agaagacgaa gatcgcgacc agcgaccaga caagagcgct cccgctatcg
     2281 gcaacgaccg ccgaggcgag gagagcgacc gagaagaacg tgaagaggta aaccagcgaa
     2341 acaagccaga agaccaggat cgcgccgagt tcggcaggcg aagggacgat tgcaaagagc
     2401 aggaggagcg cgagcgcgat gagaaccgcg atgctcatcg ccagcgccag ggcggcgaat
     2461 ccgccgagcg ccttgccggt gatcacctcg tcgcggaaaa ccggacggga gaggagggtc
     2521 ttgagtgagc ccgaccagcg ttcccgcgtg accaggtcga acccggtcgc gatcgcaagc
     2581 agcgggccgt aactcgcgac catcgaggag aggttgagga agaccgtaag gagggagggc
     2641 ttctccggca tccagtcggg gtaacccagg aatttctgcg ccggcgcggt accgtcagag
     2701 tatgccgcga gtttctcgct atacttctcg acgccctcgt agagggagac ggagcagagg
     2761 atcaggaaga ggagaaggac aagggtgaac cgccggctcg cgaggtggtc ggcaaactct
     2821 ttccctgcca cgaggaacat gcggttcgga tcgggtaccc tctctgactt cctgtcgtct
     2881 tccaccggac cactacctcc ccgggttgta agatgcagag agaacctctc cggaatgggc
     2941 gtttatccat atttcggccg ttcgcggcgg gtcctgcgac cggtaccgct cgtcgtcgaa
     3001 ctcgatgcgc caggcaagag gcaccgcgac cggttccttt gatgggcgtt gctgtgcggt
     3061 caggtcgtac ca
//
LOCUS       DAJB01000206            8001 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_41037, whole genome shotgun sequence.
ACCESSION   DAJB01000206 DAJB01000000
VERSION     DAJB01000206.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_41037"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1497 a         2646 c         2329 g         1525 t
ORIGIN      
        1 aattcataga gttaaatgac aacgagtact agtgctcgga agtaggtctg tggagttgct
       61 ccggctgggt atgtgccgga atgggtggat gccactttgt aataaccttc tgaagggaac
      121 cattttactt tgagcgtctc cacgaaactg atccagactc cagaatcaga tccggaatct
      181 acctcactcc atgaagatcc gtcactactg taggaggtca tcccacaatc atatgaggtc
      241 tttaggttgt cccaaaacgc ttatgcggcc gtgccgtcca caggctcgac cgatgaaaat
      301 cggtaacccg gcttccattt ccaccttcaa accgattttc atgtgaaact cttccgcaca
      361 accgccccgg caaccccggc ggcggtcccg agaagaatat agagataccc cagggcgatc
      421 ccggccatcc ccgcgagccc ggcgacgaac cccgtgtcgt gcgacgcggt ccagaggaat
      481 gggagcagaa aaaggccggt tccgaagagt gccgcgacaa gcccggcctc aaggccgttt
      541 cgtgcccctc ccttcgtgga atagcctgcg atgaacccac cgacgagcgg ggcggccagg
      601 agcagcgggc cgagaaaggc aacgatgaga gccgacccgg cgatgacggc cgtgccgatg
      661 ccgatcccga gccatcgtgt tcgctcagcg gcaccggcag tgcccctcct gataccgttt
      721 cgcaccgccg tccccgccgc cccgccgacg gcgttgtagg ggaggaggag tgccgtgagg
      781 atcagagcgt agaagcccaa cgtcatccag aacggaggat actgcggctc gctgttcacc
      841 aggctcatga gggttgttgc ggaggcaata aacagggccg cgagcactcc gcacacggcc
      901 ccggtgatgg ccccgtcctt caccgagccc ggggtgagga gcccggcgac gaggcccccg
      961 ccgaagagga gcgctaccgt catgaccgga cccgggaggg tccccagcag accgagaagc
     1021 aagaccgccc cgatcgccag cgctcccacg atcaccccga aaaagcgttc gccgacggta
     1081 cgcgatacca tggggggatc tcgccccgcc atgcgttaac cattctggtt tgtccctgtc
     1141 gcaggagcag aggtattata caccagggga ggtatggaac tgcaacagag ccgcggccgg
     1201 agagcgaccc tcccggcccc ggactgctcc tcaaccggac ctgtatatta tgggaatcac
     1261 agaccactgc cggcgcaccg tcgccttcac ccgggatctc atgcggcagc cggtgaagtc
     1321 cggcaaagac ctccggttct ccgacctcgc cttcttcctc cggttcgccc ggccgatctg
     1381 gagagtcggc gtgctggcgt tcgcagcgac ggctcttgtc tccgccgcaa agaccgtcct
     1441 cccgctcagc atcaaggtct tcatcgacaa catcctgctc ggggaagccc ccgcccccga
     1501 cgccctcctc gcaggggccg gcggactcct ctcatcgctc aacgccctca tcgtcgccct
     1561 cctggtgctc gggttcttca ccggcggcat ggatctcgtc cggaactact ggaccgcccg
     1621 gttccgggaa gactacgcct tcaacctcca gaccaccctc atcgagcacg tcttaagttt
     1681 cccgctctcg tacttcaagt cgcagcagac cggctacatc atgtcccgcc tctccgacga
     1741 catccagatc ctgcagtata ccgtctccca gtacctcccc cagatcgtcg caaacgccct
     1801 ctacgtcacc ttcaccttcg ccatcctctg cattttaaac ctcagactga cgctcgtcgt
     1861 cgtcgccttc atccccctct acctgctggt caacgccgtc ttcatccggc ggatccgtgc
     1921 cgccacccac cgggagcgcg agcggcaggc ctacgtctcg cgggacctcc aggaggtgat
     1981 cgccggcatc gacaccgtaa agtcgcacgc cgcagaggac cgggaggtgc gcaaggtctc
     2041 cgggacgctc cggaacatgg tcaatgcccg gatcaaaaac acgatgctct ccgcattctc
     2101 ggagtatttc aggatcggca cgatgtcgct catgatcatc gccgtcttct ggttcggcgg
     2161 gaacgaggtg ctcgccggtg ccatgaccat gggggacttc gtcgcgttcg cggtctacat
     2221 cgtgacgttc gctcccaccg tcaacacgtt cttcgccttc cccgtcgtca tgcagcccgc
     2281 actcacgtcc gccgcccgcc tgcgcgacct cttcgggatg gacggtgaag cgggcgccgg
     2341 cgggattgtg cccgccacgg tccagggcca aatcgagttt gccggcgtcc ggttcggcta
     2401 caccgggtcc gaaccggtcc tttcggacgc aaacttcgtc gcccgtcccg gtgaggtggt
     2461 cgccgtcgtc gggcggaccg gtgtggggaa gacgacgctc gtcaacctga tcctccgggc
     2521 gttcgcgccg caggaaggca cgatcaccct cgataagcgc gaccttttat cgctcgaccc
     2581 ccggtggctg cggcggcaga tctcgctcgt ctcacaggac ctcttcctct tccacaccac
     2641 catcgaggag aacatccgct acagcaggcc tgaggcaacc ttcgaggatg tcataggagc
     2701 cgcgcggaag gcgcagatcc acgacgacat cgtgggattt ccagacggct accagacgat
     2761 cgtcggggag cgggggaccc agctctccgt cggccagcgg cagcgggtcg ccatcgcccg
     2821 ggcgttcctg aaggacgcac ccatcctcat cctcgacgag cccacatccg cactggacct
     2881 gcagaccgag gaccagatcc ggcagacact caaaaccctc gtccgggacc ggacgaccat
     2941 cctcgtctct caccgcccat cgctcctggc cctcgccgac cgcgtcctct cgatcgaagg
     3001 cggacgggtg cgggagtgcc ggggagccga tgccttccgg atacctgatg ccgaccgcac
     3061 acaccgctcc ggtcagcctg ctgccccctg attcaccggc cgcagtgtga gtctggcgac
     3121 gtcaaccgcg actactcgac cgggtgaccc gcggcccgga gagcggctac gatgatgcag
     3181 tagccggcga cggtcccgat gagcgttgat gcgacgctag cgaggtcgtt gccccagtgc
     3241 atgcggctcc accaccccgc cgcgctgccg atgaggaagg acgtaaaaat gatgaggatc
     3301 tcctctctgt caagcgttgc catgagatgg agaactatcg gctctctcca gccatagcat
     3361 ttgcgcaggc ccccgagaac agggttatat gccggccggc aacgatcaat atgaagaatg
     3421 ttcatcgcct gccacctctt cctcggcctc atcctgggcc tcgcgatcgc cgaccgcctg
     3481 ggcgaccggc ggctcatcgg cttctccgct ctcggggccg tcctccccga cctgctcgat
     3541 aaaccggtgg gccacctcct tcttgcggag tcgctcaact ccgggcggat attcgggcac
     3601 gggctgctct tcctcacgct cctcgccatc gcaggcatcg ccctcgagag gcagcggggg
     3661 tcgttcgccc tcctcgccgt cgcagcgggc gtgctctccc accaggtcct cgacgcgatg
     3721 tgggcgatac ccgtcacctg gtactnnatc ctggcggaga tctcttcgct ctcggagtgg
     3781 atattcctcg tggcatcggc cggcatcgtc ctcgccgcct gccggggctt cggccccgac
     3841 ctccccgggg ttacccgtac actcgcccgc gtctccgtcc cgctccttgg actcctcgtc
     3901 gccgcctccc tctacgcctg ggcggccggc atcccggaga gtatcctgat ggccggcgca
     3961 gggccggaag attacctgct gctcgcggcg gtgggtgctg tcgggatcat cggggcgatc
     4021 aggcaccgcg acctcctgga cgccggatag ggccgtcact tgagaagggt gatggtatcg
     4081 atgctgcccg ggacggtcct ctccacccgg agcgcggccc tccctgccgg tttgatctcg
     4141 acggtaaacg acctgtacgg catgcagtcg gttctgatcg ccggtgtaat attcagcata
     4201 tactgctccc cggcctcgat aagaaggtcg gcgtcgctgt tcaaccgctc ctggatgctc
     4261 cagaatccgt atccaggaga gacgtcgacc agaggcacgc tctgagcaac gatctcccgg
     4321 tgcgtagaac cgacaaaccg cacagatacc gtggcgaaat cgatcggctc gctgccggcg
     4381 gtgagcccga ccgggacgat gacggaatcg atatatccag gcgtgttgct gacgccgtag
     4441 accataccgg tcacggtgac gctcgacccc gcctcccgga taccctggtg aatggttgac
     4501 tggctctccc cggagaagag aatacccgtc gtcaggacga cataggcaaa gacggctgca
     4561 acgatgacga atgcaatgag tacaatcgcc gcttcaagcc cggtgaatcc ggtctccggc
     4621 aggccccggt gggggcccgt cgcgtcagag gagctcgacg tagacatttt tcccgatgcc
     4681 ggcaggaatt gttctggtca gtgaagcggt cgccccttca gcggcggtca tatcgagggt
     4741 gaacgtatcc ccccgcctga tgcccacgct tcccacattc agtttcaccg tgacgacctc
     4801 ccccgcttcc aggaggtctc caggatcttt ttggtaccgc caggtctcgg ttacgtgagg
     4861 gtcgctggcg gggaacgtga caaggctctc ttttgtgacg atggtgtagg tcatgctccc
     4921 catatcgact gcagcgagat cggtcgcggt ctcaaggtcg aactcaacaa agtcgagcag
     4981 gcccgtggca tccagtttgg cgatgaccgc gtaccccggg cggaggccgg acccggcttc
     5041 gccgagagcg gcatgcatca cctcctggct cttctgcgat gtcgtcatgc ctgtcccgag
     5101 tacaacgaac gcgaagaccg atgcaacgac aacgaaggca atgaagatga tggccgcctc
     5161 aagtcccgta aaagcccgat cgctcctctc ttcccggggc ataatatttg cgagttgatc
     5221 gcactccgat taaaagatgc tggtatctcc cgcggggtgc tgctacaggc cccccggagg
     5281 ctctcagaga acttatttat agcatggctt tttatggggg taccagaatg acgtctgaaa
     5341 ccaccgcagg ccctctcacc gattccttca acccccgcac catcccgctg gatttcgtga
     5401 gcatttaccc gggaatcgtt ctcgccctct cgtttggcct ctccatcgcc gtcgtccccc
     5461 tcatcggcct cgcgctgaac tacacgccct gggggatccg gctcgaccct atcgccgtca
     5521 cgctcgtggg attcagtctc gtcattggtc ttatcgcccg gttccgacgc cgccgcctgc
     5581 ttaaagagag tcgcctccgg gtcccggccg gcacctgcct cgccgcgata cgggaggagt
     5641 cctccggtaa ttccgcgttc cgggtcttaa acatcgtcct cgttgctgcc gtcctcgtgg
     5701 ccatcgcaac gacggtcttt atcgtggtga ccccaagaga cggcgagaaa tacaccgagt
     5761 tctacatcct cgggccgaag gggaaggcag ccgattaccc gaccgagttc atggccggaa
     5821 caccgcagac ggtcatcatc gggatcggga accacgagta ccgggagatc acctacacgg
     5881 tggagacgta tgccgtcgag agcaggttca ataacgcgac aaaccagtcg acgatcgtct
     5941 cggcgacacc gctcgaccgg ttctccgtca cggtgccgca taaccagacc gtagagcagc
     6001 cctactcctt cagggtcgtg gattcggacg cgaacaggct cgagttcctc ctcttcaaag
     6061 agacgccccc caccgacatc cccgacagca gcctcaccga ggccgcttac cgcgacctgc
     6121 acctctggct gcgggtgcat tgagaggaac gagggcaact cctggtactc ctccaaatat
     6181 ttatccattc taacggcgca tgcttgtttg aggtacacag atgaaacgcg aaattatggt
     6241 cgcctccctt ctggtcctga cgcttatcgt ggcccctgcc ggagctttca gcgcagacaa
     6301 ccttctgata accgtggacg atgacgggag cgcaaacatc acctttgagt ataccatctc
     6361 gtggatcgag aggatcgccg tcttcttcaa gatcgccgag ccggagcagg aactgaaatc
     6421 cgcgctcgag gagacgttgg gggttcccgt aacggtgacc tcggcagaga gcgacaacgc
     6481 ttcattcctg gtccagatct ttgcaaaggt caggagcacc gatgagggga gcgtctacac
     6541 gacacccgga ctcgacttca ccagggcaca ggagatgctc gacagttact ggtttgcgcc
     6601 gctggtcgag gccgacttct ccccggacct gacggtggtt cggttcccgg acggctacga
     6661 ggagaccttc gcaaaccaga gcgccattcc ggcactctct cacgcattgg cgtgagcaga
     6721 aatctctctt tttttcggta gtgccctacg aaacaactga taataacgtt gttgagatcc
     6781 catccgggtg ttcccgattg attgcaatcc ggggcacggc tttccaccgg gtnnggccgg
     6841 gcggggtggg ggttacacgt ctacttgtgg gatggagaca ggggaggggg agaccccctc
     6901 cccgtcagcc ccacggtcca cttgcagggg catgcccggg gaacaaggcc gttcccgggg
     6961 tgatccctca tccggggtct gtttttcatc ggtatgcttc cacacagaag gcaggccatg
     7021 agggatgcga agggcgtgaa agggaattca tagacttaaa tgacaatgag tattagcctg
     7081 ccgcaggcgt ggccctcgaa cgatgggagc ccctatagtc gctgaccgga gaagaaggcg
     7141 gcatctgcca ggccgtaatc tatctaaaaa aaggagaaat cttccgttta ctcgaagatg
     7201 ttgaagatct ggtcgctgcc ggtgtcggag agacgcttgc gctctgtctg ttcctcgacg
     7261 agcgcgagca ccgggagttc catgtccaca ccgggggtgt ggccgaacat atcctgaagg
     7321 tcggtctgca gggcgtgcat gaagagcgcg ttcgggatat ccatcgcgca gtgctcttcg
     7381 cactggccgc agttgatgca cgagtccgat atgtgggcga accggatcag gtggaacatg
     7441 aagggcggcg ggacctgacc gggcgtgacc aggtaggatt tcttcgtgct gcactcgacg
     7501 cagtagcaga tcgggcagtt ctcgatgcac tgatagcact tgatgcaccg gctcgtcgcg
     7561 tccatgatct tcttgagacg gtccttgccc tcgccgagcg ccgcgaaatc cttcgcccgc
     7621 cacttgtcgc cgagcttcag catcgcgttc tcgaccttgc cgcggatctc gatacccttc
     7681 gggttcgcag caccggtctt gaggactccg gccgtcacgg caccgtccag caggtttgca
     7741 cccttctcgg agcagacctc aacgaacgtg gccttgccgg ccgactcacc gatgacaccc
     7801 cagttgccgc aggcgagatc cgcctggcgc gggaccttca tcttgcagcg gcggcagttg
     7861 gcccggcggc cgaacccttc ttccttgacg acgtcgtcag ggttgacccc gaacttctcg
     7921 gcgatcatct tccgggcggc cacggggctg acggagccgc cgcagttgag gccgaccatc
     7981 aggacgttgt ccaggttgac c
//
LOCUS       DAJB01000207           12049 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_72038, whole genome shotgun sequence.
ACCESSION   DAJB01000207 DAJB01000000
VERSION     DAJB01000207.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_72038"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    3237..3268
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3617..3631
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2246 a         3761 c         3783 g         2212 t
ORIGIN      
        1 gcccttcagg gcggggtagt tcacgccggc agcacaggca ggatcgagtt cgacggtgcc
       61 ccggaccccc cgggcgccga ggggctgtat gggcatattg cgggggtcgc ggaaggggga
      121 gcggacgatg ccgatggggg tgaatacgat gccggtcatg ggtgtcactc aacggcgttg
      181 tgcggcaaca tcaaagaggg tttgcgtccg ggcagagtat ggggaacggt tattatcggc
      241 acgtgcgttc atactgcttc tccactcgca ccactacatg tgccggatta tttatacgtt
      301 ctgggaccgg ccggaaacat ctatacagca gccgctctgg caacggcagg caggagcagc
      361 gcgttgaccg catggcagat gccgttcgtg caccacgcat cggattccac gaccggcacg
      421 ttgttgacca gcaccccctc cggcgcaacc tccacggtca ggtctccccc ctggagcgac
      481 cgcagggccc gtataccgag caagtcaatc atggagtgca cccccgggac aatatggtaa
      541 ttcaggacgc tggcgagccg gtcggcgttc tggcggatcg catcgagttc tgagggggat
      601 attgcccgga aagcatcgtc atccgggacg aagagcgtat agggcccttc gttgctcaac
      661 cgctcttcca tgccggctac ctgtacgagg tcaaggaacg tggcaaacga tccggattcc
      721 cgtagagtcg cgaggatact cttcatacga accaactccc ggcgggagaa gataaaggta
      781 agggtggtcg ggcagcaggg ttgagcctga gacgagagcc gccgcctcag accagtttct
      841 tcacccgtgc ctcgaccgcc cgcggcatga gcacccggtc gatgatatgg tagatgccgt
      901 tcgtgcactc gatgtcgggc tggattatgg tggcgtcgtt gagccgcatc gccgccacag
      961 gtcccgtaat atccaggtga tccccctgca gggtcctgat cgcctccatc tgtgcaagct
     1021 cctgcacggt gagtttgccc cggagcacat gatacaggat catacccgcg agcctctccc
     1081 ggtcgtccgt gatagcgtcc agcacctctc cggcgaaccc ggaatacgcc tcattcgtgg
     1141 ggaacaatgc cgtatactcc ccctccccct tgaggagctc cgccacccct ccggcctgta
     1201 tcatcccgat tgaggtgctc aaccggtcgt cttcccgggc tgtctcaaag atactcttca
     1261 tagatatgcc tccagatgga cacccggatc atttagccca atatataaaa ttacgtttat
     1321 accggcacgg gctcccggga tggccggaga gggagatagg gccggattgc acacccatcc
     1381 cggcaggcgg caacgagggg aagcaccccg cacccggcgg gaaagaaagg gatttttgcc
     1441 ggacccaaaa gttctgtcag tccgggctac tttgcagcgt aggcctcgaa ggcctgcgag
     1501 agcgtcacga aggggatgcc gtcaagggcg tggatgatgc cgttgtccgc ctccatgtcc
     1561 ccctcgagca cgacggcctc gtccacctgc gtgccgcgtt cggtcttcct gaccgcgatg
     1621 acgctccctt ccacagtctg cacgacattc cttgagagca gcgattcaag cgtgcactta
     1681 tgcccgccga tcgtgaagaa gtcgatgaga tgcgagagca tctgcttatc gttcaagatc
     1741 atcgcccggt tcggctcggg gatcgcatcc catgccgagt cgaccggagc aaagaacgtc
     1801 atcggcccct cgccctgcaa cgtcttctcc tcgccgaggg tctgaatgat ctcgatgagc
     1861 cggctgaacc tgccgtcatt ccgtaatgta tcaaagatct tcgccatacc aggtaccttc
     1921 ctgtaagggc ccttcgtggt aggttattgt atatatggat tattatactg tgaatggcat
     1981 agataacagg agatacgcgc ctccgtgccc gggccgggcg gcaggcggcc ctcgatcccc
     2041 gggaagaacg ggtacatccg gttttttttg gaccggcaat gccggccggg agaaaccggg
     2101 agttcgggca acgggaaaac cgtcactccc cggtcaggtc gccgatatac cgctcgagga
     2161 gttcgaccgc ctcccactcc cgggggccgc cgcacttccg ggcgaggccg gtgtggtggt
     2221 cgatcaatcg tttaagccac gggtcgcgct tctcgacata ccgggtggca tggaccgtcg
     2281 cgtccacgac gcttgcaaaa ccccggttca ccgggtgcag ccgcaggtcg agcacctcct
     2341 cgtggaaggg gacgagccgg acgacgatcg cctcgtcgct cgaccgctcc acgtccgcgg
     2401 caaacgcagc ccaggcctcc acgtcgcgga ggcggcagac gaccatcccg tcgatcgcct
     2461 cccggacgaa ggcctcctcg gggaggtcgt cgaaggcggt ccggacgtag atcaccgggt
     2521 cgaagacgaa gttcgccacc acccagcggt cccgggcgat gttcctcgcc gtatggctcc
     2581 cccggaagag gaccatgtgc agggagccgc tccggttgat gatccccata ggagcggcgt
     2641 tgccgtcggt ggtcgcgatg acctcgttga tcccctcgct cagcagtccc attgccatcc
     2701 ctccccgagc gccacgtata ttccggcgat ggtgatgtcg gcgatcgaac ccgggttgat
     2761 gccgccggcg atgcactcgg cgtcgaaggc acacaggtcg cgccccccgc cgagcacctc
     2821 ggccgcacac cgcatcgtcc tctccgccac cgcctccccg tgcttcttcg cgatgaaggt
     2881 atcgggttcc gacgcaagga ggtcgaggaa cgccgcgacg attgcctgcc tgccgtgacc
     2941 gtgcgcatgc agcaggtccg cgcaccggcg ggtcagggca aagccgttgg tccactcccg
     3001 ggcgaccata tcccggggcg ccgagtgcgc catgatgtcg gcaagggtca tgccccgctc
     3061 ccggatcgcg gcgatcgagg ccgggtcgtt cacgtcaagg tcgtcgctct ccggcatccg
     3121 gacactggtg aggccgaacg ccgcgtagaa atcgatcgcg tcctcgacat cggtcccgga
     3181 gacaatgcgg cgtgctccgt cgatatctcc cccgaggacg agcgggatga ggaggannnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ggcgggtctc gcgaggatgg tcgaggcaag
     3301 gaagtgctcg agacgggtat ccgaatagtc atggcaccgg tctacgttcc cgggtttcgg
     3361 gtaggcgcag acctcgagca tcatcgccat ctgcgcacgt tcagctcgat ccatcgacgg
     3421 tctcttctcc atcgaatacc ctgtgcagta tcttctcgga gaggcggtgc ttgcaccgca
     3481 tgtactcccg ggcagagagg cctttactct ccagcgtcat gttgcggaac atgcagggag
     3541 aactgatctt gcagcaccag gcaaggctcc cgaaacaggt gttcttcccg tcgtcgagcg
     3601 gggttccctt caccgcnnnn nnnnnnnnnn ntcgccgccg cggcagagct gtttcggggc
     3661 gttgtaccag ccgccttcgt cggcggaccg ggtgatggcg gcatcgaggc cggcgagcgt
     3721 ccagggatcg gactgccggg caagcgagac gaggtctgcc ccatgcgaga aggcgtccat
     3781 cgccttatca aaggtgttga tggagttgtt tgcaatcagc atcagggggg aggcgttgcg
     3841 gatctggcga accttgttgt gtccgaagtc catcaggtcc acgtggagga tatccgcccc
     3901 cgccttccag acggtgcggg cgaggtcggc gtcgttcgcg gcgacacccg cccgcatctt
     3961 caccgagacg gtcacgtccg cctcttttaa ggcccggacg atctccacga gttttgccgg
     4021 atgtttgagg aggtgctcgc cgcagcctgc gtcaagcatc gcgggctgcc ggcagtgggc
     4081 gtcgatctcg tagaccacgc ggttctcaaa agcttcggcc acctcgccgt aggctgcggg
     4141 agtgctcccg cggaggttga tgcccgtaac aacgtcgctc tgttccaggg catcgatctc
     4201 ttttcccagc gcttccaggg gatcgtcgta tacaaactct ttccggcccc cctctgccat
     4261 ctgccggctt gcatcgaggg tggggccgtc gatggagtag ccgccgatga aggcggcccc
     4321 gacatgagcg gcccgctcaa gaacgtaggc cgcatccacg atccccgcca tggatgcgat
     4381 tgctatcggc gtttttacga ctcgatcgtt tatgaggagt tcaaaacgat catatgcttc
     4441 catcatgctt ctccatccgt tggtagtgag agaggatatt gatttgtatc cagcccggga
     4501 agatcgtact cctcaccgga gcggttgagc gcacagcaga gccggaaatc gtcgatagag
     4561 atgccgggca cgttcccgta atacccgagc accctgctcc agggcatgag gtatgcctcc
     4621 ttcgccctcc ctgcaccgaa ccggaactcg acttgcactc gatggccggg tagtaacggg
     4681 ggtcgaggga gtactgagta ttgcatttgc tctgcttgag ccggtaggca aacccgtgag
     4741 cctgccggtg ggcgaagaat cggttgatac agcaggtgat ctcccgttcg aagtttgcca
     4801 tcacataggt tttggagcgg ggccttaaaa ataatcggta attattttaa tatagtgtcg
     4861 tagtaccttg taacatatgg gtagaattgg aatgttactg gtcggacatg gcagcaaact
     4921 cccttacaac aaggagttga tcgagaccac cgcagggctc atcgccgaga agaccgatga
     4981 gtacctcgtc aaacccggct tcatgagcct caacaccccg acggtagtgg accagctcga
     5041 ggcgttccgg ggcgaggata tcgacatgct cgtcgtcgtc ccgctcttcc tcgcgaaagg
     5101 cgtccacatc aaccaggaca tccccaaact cctcggcctc tccgagggat cgcggatcgg
     5161 cacgttccag ttgaacggaa agacggtgcc gctcgtctac gcaagcccca tcggcagcga
     5221 cccgctcctt gccgaactga tgctgaagaa cgcgtccgac gcgatcgcga cactgaagcc
     5281 ctgaaggaaa tgcgagtcct ggtgctggat accatccacg gcggggcaga actcgccgtg
     5341 gcactccgtg gtgccggcca ccaggtggac gaggtggacg tctaccttgg acaggccggc
     5401 atccctgttg cggaggcact cgtccgcacc tacgatctgg tcaccgcacc ggtccacctc
     5461 gaccccgatc accctctcct ccggcggcac ggccccgcgg tctcccacca cgagatggtg
     5521 aagtgggtcc tcgacggccg tctcccgcac cctttcatcg agatcaccgg agcacggggg
     5581 aagaccacca ccgcccacgc tctcgcgggg ctgatgccgg gcccgggcat cctgcacacc
     5641 tcgaccggga cctaccgcta cccggagcgg gagcggctct ggaagaagag catcacgcct
     5701 gcctccctga tcgtggcggc gcgtgaggcg gagcggatcg ggggctggct ggtcgccgag
     5761 gagtcgcttg gggttaccgc agccggagac gtggcggtgc tgacctcgcc ggaggactac
     5821 ccggtcgccg cggggaagaa gcaggcgatc gtggagaaat gccggctgct ctcccgggca
     5881 cggacggtcg tgctcccgcc ggggatcgac ctgtcgggag gggtcgccgc cgacgagata
     5941 gtatctttcg acggccaggt ctgccgctac gaaggatgcg ggatcgccgg tacttttgca
     6001 aacccgctct gcaccctggc gggataccgg acagcgctct cactcgcggc cgcaaccgcc
     6061 tgcgtgctcg gaatcgatcc ggcgccgctc gcggagttcg ctgccctgcc cggccggatg
     6121 gcggcccggc gagaggggga cctcctgatc gttgataacg caaacagcgg gacgaatatg
     6181 gcgaccaccg ctgaagccgc ccgctacgcg agagaactct cgggcagcga aaccctgacg
     6241 ctcgtcatcg gcgaggaggc acgggcggtc tgcgaagggt tctctcccgg ggacgtccgg
     6301 cgggcggtat cggcggtcgg gccgacggcg accgtctacg tcggggaggg gcatgccgcg
     6361 gcgacgctgg aagcggggct cgctctcgcc cggagcatca ctcctgcggg ggcaatcgtc
     6421 ctcgcagtaa agacgtggag atagacttat ggactacatt caaccgagac cgagttcaat
     6481 tgtcgcggcg ctctacaccg cccgcgacct gggggtggac gtggcgatcc tccacggccc
     6541 gtcaggctgt tcgttcaagc acgcccgtct cctcgaagag gacggcatgc gggtgctgac
     6601 gacatcgctc gccgacaacg agttcatctt cggcgggcac gagccgctca tgcgggtgct
     6661 ccgctacgct gaggagacgt tttcaccccg gcggatcgcg gtcgtcggga cctgtgtctc
     6721 gatgatcatc ggcgaagacc tccagtccgc gatccgcgac gccgcgatcg ccaccccggc
     6781 gatcgccgtg gatatccatg ccgggtttcg ggagaacatc gacggggtga tcgcgacgct
     6841 cgagcccgcg gcggccgacg gctggatcag cgccgacgaa ctggaacgcc agcgctacct
     6901 cctttcaaaa gcaaacgagg tggagcggct gcggggagcg gcgtcccgga cctacatcga
     6961 gccttcgcgg ggcgacctca agcacgtcgc ggcagaacgg ctcgtggagc tcgccgatga
     7021 gggagctacg ggggttgcgg tcatgaacgc gaagaaagag accgcctata tgttcgccga
     7081 cgaactcctc gggctccacg atgcctgccc ggacgccgcg atcacgtatc tcgcaaacct
     7141 cgaagaccgc ggtctcccga aggtgcggga cgacgccgcc cggatcctgg ccggtatgca
     7201 ggacaggggg gtcgaccccg aactcctcgg agcgctggac gagtacggcg caaacggccc
     7261 cgccatcggg gagcggatcc gggagattgc gcccgacttc gccctcctcg tcggggtgcc
     7321 gcacgccgtc ccgccggagt atacgcaagg tatcgaggtc ttctcggtca cgaacggccc
     7381 ccggcaggtg gcgccgctcc gggagctggg gcaccggcac gtcgtggtcg agatcgacct
     7441 gcacccaaag accctcggtg tccgggacgt tgtcgagagc gagttcgggg cggtcctgcg
     7501 gagcatgcga tgaagtcctt cctcgtcgcc ggggaccggt ccgggagcgg gaagacgagc
     7561 atcaccctcg cgctctcggc ccttctctcg accacccgga ccgtccagac cttcaaggtg
     7621 gggatggact acatcgaccc ctcctacctt gcgggggtga cgggccggcc ctgccggaac
     7681 ctggacggct acgtgatgag cccgggggag gtcggtgcgg tctttgcgca cggctgccgg
     7741 ggagcggata tcgccgtcgt cgagggcgtc agagggctct tcgagggggc ggaggcgctc
     7801 accgacctcg ggagcacggc cgcgattgcc aagcaactcg acctgccggt cgttctcgtc
     7861 atcaacgccc ggagcatcac ccggagcgcg gcggcgatcg tgaagggctt tttggccttc
     7921 gatgcggacg tcgatatccg cggagtcatc ttaaacaaca tcaccggcac ccgccaccag
     7981 gagaaggcgg tccgggcgat cgagcactac tgcggcattc ccgtcgtcgg cgccatcccc
     8041 cggacggcag agatggaact cgcgatgcgc cacctaggtc tcgtccccta ccgggagggg
     8101 cagggaaacg aggcgttctt agaccggatc gaggtggtga agcggatgat cggcgatcac
     8161 gtcgacctcg acgccctgct tgcgctcgcc cgggagtctc taccgccggc ggaggagggg
     8221 gccatgtatg cggcttgcga tccggacgtc cggatcggcg tcgcgctgga cgaggccttc
     8281 aacttctact acgccgacct cttcgatatc ctggcgtcgt tcggcgctga ggtggtcccg
     8341 ttctccccgg ttcacgaccg gctgccggag gccgacggct acatcctcgg cggcggctac
     8401 ccggagctct tcggccctga actcgaggcg aacaccgcga tgcgggaggg attccgcgag
     8461 gtctcccgga acggcacgcc gatctacgcg gagtgcgggg ggctcatcta cctcaccgac
     8521 cggatggtcc tcgcccgcgg gtttatggat gcggatacgg agaaggccta tgaactcgcc
     8581 ggggtctttt ccggcgagac ccggatgccc gagaggcgga tgctcggcta cgtggtgggg
     8641 acgagcgcgg ccgggagcct gatgggcgag gcggcgttca agggtcacga gttccactac
     8701 agcagcgtcc gccttgcacc cgggacgcac tacgcctacc ggctcagccg ggggagcggc
     8761 atcgagggcg ggtgtgacgg tgcggtcagc gaccggacgc tcgggagcta cacgcacctc
     8821 cacccggtgg cgagccgggg aatgctcgca cacttcgtgg actgctgccg gacataggca
     8881 tgagcggcgg ggaggccccc gcactatttt taccggtcac cgcagggtgg tccgacggta
     8941 taaaacgcaa aacatacaaa caaaccggaa ccatactggt tgcatgatca tttaccttga
     9001 cggcagatac gtccccgaag aggaggcgaa ggtctcggtc ttcgaccacg gtctcctcta
     9061 cggcgacggc gttttcgagg ggatacgcgc ctacaacgga agggtcttcc gtcttgacga
     9121 gcatcttgca cggctctacg actcggcaaa gacgatcgac cttgccgttc cgatcacgag
     9181 agaggagatg gccgaggcga tcaaggagac cctgcggaag aacaacttaa cggacgccta
     9241 catccgcccg atcgtgacga ggggcaaggg cgacctcggc ctcgaccccc ggaagtgccc
     9301 gaaaccgacc gtcatcatca tcgccgtctc gtggggcgcg atgtacggcg acctctacga
     9361 gaagggcctc cgggcggtct gcgtctcggt ccggcgcacc cctccggagt cgatgccgcc
     9421 gaacgtcaaa agcctcaatt acttaaacaa catcctcgcg aagatcgagg cgaactaccg
     9481 cggcgtggac gaggcgatct tcttcgacat ccacggcaac gtctcggagg ggtcggggga
     9541 caacatcttc gtcgtcaaga acggcgtcat catcaccccg ccgaccctga acaacctgcg
     9601 cggcgtcacc cgcctggtcg tgctggagct tgcggcgtcg atgggcctca ccctcctcga
     9661 gcggaacctg ggctacttcg atctctacgc ggccgacgag gtcttcgtca cgggaaccgc
     9721 ggcggaggtc ggcccgatcc gcgagatcga cggcagggtc atcgggaacg gcaaacccgg
     9781 ccccatcacc cggcagttga tggcggtcta caagaccgcc acccagaagg aaggctcccc
     9841 gatcaacagg tgaggaactc cggtctccgg ccggcccccc gggcctcctc cggcacaact
     9901 tttttatccc cgggcatcga agttgtatgg cacgttgtgc atgctgctat agtgtagggg
     9961 ccaatcatgt cggcctttca cgccgacgac aggggttcga atccccttag cagcactccc
    10021 gatttcgatt ttgtccgatt tttgagactc tttacccatt agtcttgagt ataggtcgtt
    10081 aatcttagat tcaacatcag aaagacgttt ttcaagggag tccggcagga gaaacgccat
    10141 cgaaacgacg ccaagtgacg gcactacgta taccggttca atcgtcgtgc cgttcgtcga
    10201 cgtgagtttg gtctcctcga tgcccatcat tttggggatg ttggggccgt gaatatcgag
    10261 atcgaggagg cctgtctggt agccgtggtt cgcaagcgcc attgcaaggt tcgcagccac
    10321 cgttgtcttg ccgacgcccc ctttcccgct cagcaccagg atgacgtgct tgacgctcac
    10381 ggcaaccttt tcaaacgggt tcttctgttg ctgtccggta tccggagcgt tgttgctccc
    10441 ggtttcggca tttttcgttg tattgtctgt ttctaccata acataccctc aaaatcttat
    10501 ctccggagaa aggacgattc cgttcaccgg ttacgaagtg attgtgaacc cacatggggg
    10561 ttcttcctga accagaccat gcaatatggc tcgtacaata aagttaacac tgttaacaga
    10621 aaacaatatt gatgcttaca tgcctgaccc ccatcccggt gtgagacgat tcatggcact
    10681 cattggattt gctccctgaa cagttctccg ccggggaggt gcgccggttc tctttttcgt
    10741 gccgggtgat gaccggcgag ccgtacgccg ggatggtgct tgcccggttc caggacattg
    10801 ctcctgaatg cgacgaggca gtgcggagcg cctggaacgt gagggcaaaa gtcagtgccg
    10861 agtcggtctt tgccggcatc ggggaactga tcccctatgg catctgggtc tgtaaaccgg
    10921 acggcgccgt gctctatctt tcggcctcgt gaggatgact ctcgaggagt gccagcacgc
    10981 cgggtggctt gagtccatcc ggctggagga tgcggcggag gtgcttgccg actggaagca
    11041 gtgcctcaag accggatacc ggtgggatcg caagttaaag gtccgggaca ccgacggggg
    11101 ataccatatc gtcctctccc ggggggtccc cgtccgggac gaaaccggca gaatcaccct
    11161 ctgggccggg gttaacctcg acgtcaccgc gagaaggcgc gctgaacacc tcaccgccga
    11221 ccgggcagca caacaggcag ccatcgcgga actcggccag ttcgctctcg ccggggcaga
    11281 accttccgag ctgatggatg ccgcggtccg ggaggtggcc gagcacctgg gggtcgctta
    11341 cgccaaagtg ctgcgatacc tccccgacga gaatgcattc gtgctcgagg cagcggttgg
    11401 gtttaacgga tgcgatgtcg gcacgaaaag ggtggagggg gggaccgact cccaggcagg
    11461 gtatacgctc ctctcccatg aaccggtgac cgtccaagac ctcaggaccg agacacgatt
    11521 ccatggcccg gccctcctca gggatgcggg catcacgagc ggtatcagcg tcatcatcca
    11581 gggagataaa gcgccatacg gggtattcgg ggcccacacc cgggagcagc ggatatttac
    11641 acgtgacgac atcagcttcg tccaggcggc cgcaaacatt ctggcacagg ggatcgagcg
    11701 gagagcggct gaagaggcgc tcacgaagag cgaggagaag ttccgcgaga tcgcacagcg
    11761 gagcttcgat atgatctaca cctgctacac tgacggcggg atctcctata tgtctccggc
    11821 agtgacccgt atccttggct atactcccgc tgaactcatc ggctcccgct gccgcgacta
    11881 catcgtcgaa taacaaggtg ggattgctcc caccccgtct tccaggaacc caccttgcga
    11941 ctttcaccgc agtaggctcg cgcattcctc tacccattgc tgggcagcaa cgcaaaatag
    12001 gataatgtag tttgaatcca tggtgtttca ccagattcca ttttccttc
//
LOCUS       DAJB01000208            8142 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_41034, whole genome shotgun sequence.
ACCESSION   DAJB01000208 DAJB01000000
VERSION     DAJB01000208.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_41034"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1638 a         2538 c         2563 g         1399 t
ORIGIN      
        1 ccccgcgagc cccgcggaga taaagttatt ttatcgggaa cgactgcccg gcctcctggt
       61 cctgcgccgg gagagagtag tgcagctgga caaagaccca cgcatggcct gtcccgcgga
      121 gcaccgccgt cagccgcccg ttcagggtct gcagggcgcc gtcgacgacg atatggtagg
      181 tcatacctgc catgacccag gcgaccgttc cctcggcgcc gatatggatg tcgcagaact
      241 caagcgcgat catctccgcc tgagaaaagt ctcgttcaag gtgccggcga tacgcctcct
      301 tcccgatcgc cttctcgtcg gggccggtcc cgaaaccccg gaaatcaggg tcggtaagcg
      361 ccatcaggcc atcgatgtcc ttctccgccg tggccttcgc catccgccgg agcaccgcca
      421 tgatctggtt ctgtgtctgt tcgctgactt gcatgaagag tactctcgcc gaaaagaaga
      481 tgtgcctttc gagtatgcac gccaatcagg ccgggagtgc atcccgcggg gttccgcccg
      541 ccgggtcggt gtcggtgcga tcccgttcca tcgaccggag gaacgcccgg aactccggtg
      601 accgggctgc ccggacgagg cttgcgacgc cgtcgtctgc agcaagttcc ttccggatca
      661 ggagctcgca cgactcgtac ccaagcgggg tgaaccggag ccccgcctcc cgtgccatgg
      721 gggccgggca gacacctgcg tccgcaaacc cgttgccgac ggccgcggcg acggcaccgg
      781 ccgtccgcac ctcctgctcg tatccggcga tgcggccggc atcgataccc tgccggtcca
      841 gccacgcatc gaggaggatc cgcgccgccg tgcctttcgg gcggttgacg aaccgcagcg
      901 atgcaaggcc gcctgcatcg aggagccccg ccgacgcaat ccccagttcc gtccgggcca
      961 cctgcacccg caggaggtcc acgtccggca ggtaccggag cacctggtcg ttccaggccg
     1021 cttcggtctc gggaatgcag atcgaagcgg catggcaggt gttcgcccgc acggccagca
     1081 ccgctccaac cgtcgatgcg ttgcagcagt ggagcaggta gccggtctcc gagaggaggt
     1141 ttctgagttc ggcgagggcg ggatcgcgct ggccgacaca gacaagcgtg cgggcgatct
     1201 cgggcggcgg gacggtgagc cggacgtgca cctcctcgcc cgcctcgatc ccctcgcgag
     1261 cggcggggac atgaaggtag ccgttcgccc ggaccaccgc catctggatg ccgccccccc
     1321 ggggatgggg ggtcgcccag caggtcccgt tcacccgacc gaccgagacc gggacgaact
     1381 cgtcgaaccc caggtcggac gccagtctcc ttgagagccg gacgtccatc tcctcgcccg
     1441 ggagcggcga gagcccccac cacgcaagca ggttcccggc aacctcgcgg agaacggtct
     1501 gtgcggcgac gggatacccc ggcatcccga ggaccggctt gccgccgacg ctcgcgagca
     1561 gcaccggttt tcccggccgg accgcgatgc cgtggaatac gatctccccg agcgcgccga
     1621 cgacgtcccg ggagaagtcc cgggtgccgg ccgacgagcc tgccgagagg atgacgaggt
     1681 cgttttcggc gaccgccgtc tgcaccgcat cgctgatccg gtccggatcg tcgggaacga
     1741 tcccgtaacg gcggcaggtc gctcccatcc gggagaggta ggcctccgcc atgagggtgt
     1801 tcgtctcgat cgtctggccg ggcgcgggcg ctacaccgag cggcacgagt tcgctccccg
     1861 tcgggacgat cccgacccgg accgaccgga cggcgagccg ggcaatgccg taggtcgcga
     1921 gcgcgccgag atcgaacggc cggacctggt ggcctttcgg gaggacgagt tcgccggccc
     1981 ggatatcctc accggcatgc cggacgtgct gccaggacgc ggcggacttc ctgacccagg
     2041 gtatgccgcc ttcatcccag gtatcctcga tcatgacgac ggcgtcaaac gacggaggga
     2101 gcacttcgcc ggtattgatg cgagcagacg caagaagagg cagcggccgc tggtcctgcg
     2161 ccccgagtgt ctcgctgctc ttcaccgcgt agccgtcgaa cctcgcgata tcggccccgg
     2221 ggatagaata ctctgcatac agcggttcgg ccgtcaccct gccgaccgag aggtgcagcg
     2281 gcacggtctc ggcgtgaccc ggggaaggaa acgcctgccg catcgccgcg agcgcttccg
     2341 cgagcggggt aagattcagg taccggcgtg acatgctctc gactccttga tggtgtgccg
     2401 ggttgaggag gttctcctcc ccgtcccggc aggtatagat acatacgtta ctttcgatta
     2461 aaaggtaact acctccctat cggccgcgcc gggagcggat cttgcagtcg aagagtgatg
     2521 cctcgctctc tgcaaggatc gtttcgatac tctccggaat ggagaggagg cagaagaccg
     2581 cctccttccc ggggccggca aagacgtagc ggtttgtccc ggtacacccc agccggcgat
     2641 ttcccgggct cgcgccggtg atcccgatga gtgcatcggc gatgcaggga tcggcgccga
     2701 gcgtgaccga gacccgcgga tgccgtaggt cgcttccgaa tatgcgcgtc gccgcctccg
     2761 cgacccgcac cgcgagcgcc acgcccgggc agagttcccc gtgatagttc acggcctcgc
     2821 ggatgaggcc ctcccggtat tcgccggggc tcatcttccg gatcttttcc gtccggtcgt
     2881 ggccggtcgc ggagaagacg cagtcccatc cggtctgata gggcttgagg tcgatcaccg
     2941 gcgtcccgtc gatcaggtcg acgtttgcga gtagaagaaa ccgcccctcc cgcacgccgc
     3001 gcagccgcac caccgagaca gagagggggt ttggccgcac cggtgagcgg agggagaaga
     3061 cacccttctc cgggaggtcg cccgagacct tccgggccac cgccttgagg actctccggt
     3121 ccgcctcatg gagccagcag atcaggatca ggtgcgaact ctcctcgacg ccggagagcg
     3181 cccgggcgta cccgggaaag acctcgatct cggcgcagac gccctggacc ggcatatcgc
     3241 tgcgggaacg gatgcttgag tgcacgagcc cgatagggaa gagttccatg cggtgccttc
     3301 tctgtcgatg agatttatgc ccttcctcgc aggaagggga gggagacctc ttcagtacgc
     3361 cgtcttctcg tagtaggact gggatcggca gtcgacacag gccccgtcga cgagcaggtt
     3421 gttaggaacc atttcgccgc agatggagca gattccagac ttccagggcc atttttgagg
     3481 cactttcacc cgcaccctct cgtacgagag gaagtcgcgc cctgcatcga tgatctcatc
     3541 gatcagcgcg atgccccggg ttctcgggtc gaagaggggg tctttggtgt accagagggc
     3601 aaacgttgga tatcgcccga tcttctcgcc gtcgacgaag acccggattc cattcacgta
     3661 gggggcgtcg gccggcccgt tcatggtgat ggcgaacttt ccgatcggga gcacccggag
     3721 gcggtggttg ccggtcgtgc agtgggcaat cacctgcaga gggtcgggca ggcatttcgt
     3781 ggtctcgcag acggcgtaga tcttctcgtc ctcgggaggg ttcaggagtt ccagggcata
     3841 gtccaccatg gagactccga ccaggagccc gggtgccgcg aaggtgtgaa agtcgataca
     3901 ttgccggaaa tgctctatca actccggagc gaagcctttt gacttgagcc ggacttcgat
     3961 atcgttccat gtgtgattca gtctcggttg cacggaagag tatttggtca aggttagttg
     4021 taaatatttt ggatcattca accacattta gtttacatga aaatggagaa tacggcgtat
     4081 taccgatgag acatcgttga atcaaataat attgaatatt atggcgatta acctgtgtcc
     4141 agcgggtcac tggttatgaa tatcaactat acaccaccag attaggtgaa atgttaaaag
     4201 aatatgttta aatttatata ctttggagta gagtaaaaca aatgtgagca tgtcggtcct
     4261 ttcccgacat gtgaggcaac cgacgttgaa tgcaacaatc gggagaatgt gaatgataga
     4321 atccaatgga aagttacgct acgttcccac gacctgcccg tactgcggtg taggatgcgg
     4381 gctcaacctc gtggtgaacg acggcaagct cgtaggagtg gaacctctga agaggagccc
     4441 ggtgaacgaa ggaaaactct gccccaaggg agccacctgc tgggagttcg tgcagagccc
     4501 cgaccggctg acaacgcccc tcatcaagaa gaacggcaag ttcgaggagg cgtcctggga
     4561 tgaggcctac gacctgatcg cctcgaagtt caaggagacc tacgaaaagt tcgggccgaa
     4621 gtccctcggg ttccaggttt cgtgccggac cccgaacgag gaatgctaca tcatgcagaa
     4681 actcgcccgg gtggccttca agaccaacaa catcgacaac tgcgcgcgta tctgccacgg
     4741 gccgtccgtc gcagggctct cgctctcgtt cggctcgggt gccgcgacga accccttcga
     4801 ggacctcttg aacgccgacg tcatcttcct gatagggtcg aaccccattg aggcgcaccc
     4861 gctcgcaggc cgccggctcg tccaggcaaa gaaggcaggc aagaagatca tcgtctgcga
     4921 cccgcgctac acgcccacgg cgcgcctggc cgacgagtac gtccgctaca acccctcgac
     4981 gaacattgcc ctgatcaact cgatcatgta ctggatcatc cagcagaacc tccatgacaa
     5041 ggagttcatc gagaagcgga cgacgggatt cgaggatctc aagaagaccg tggagaacta
     5101 cgcggacgtc gagtcgatca ccggcgttcc caccgagcgg gtcaaggaga tcgcccggat
     5161 ctacgcaagc gcaaagagcg cggtggtcat ctactgcctc ggcatcacgg aactcaccac
     5221 cggcactgac aacgtccggt cgcttggaaa cctctcgatg ctcaccggca acgtcggcag
     5281 gccgggaacc ggggtcaacc cgctccgcgg ccagaacaac gtccagggcg cctgcgacat
     5341 gggtgcttac ccgaacgtct tctccggcta ccagaagtgc gaagacgacg ccacccggaa
     5401 gcggatggag gatctctggg gtgtcaccgg gcttgcgagc gagtacggcg tgaccctgac
     5461 cgaacagatc acccagtgcg gcgacccgat caaggcgatg tacatcttcg ccttgaaccc
     5521 ggtcgtctcc taccccgact cgaaccacgt catgcggtcg cttgagaagc tggacttcct
     5581 ggtcgtgcag gacatcttca tgaccgagac gacgaagtac gccgatgtta tcctccccgg
     5641 agcatccttc gcnnagaagg acgggacgtt caccagcggc gagcggcgca tcaaccgtgt
     5701 ccgcaaggcc gtagagcccc cgggcgatgc aaaggaagac tggcagatct tcgtggatct
     5761 cgcccacaaa ctcggcctcc agggctttga cttcaactcg ccggaggata tctggaacga
     5821 cgtgcggcgg gtaactccgt cgatggccgg catcagctac gcaaggatgg agaagccgga
     5881 gtcggtacac tggccctgcc ccgctgagga ccatccggga accccgatcc tgcaccgcga
     5941 gaagttctcg tccgccgacg gcctcggcca cttcttcggt ctcgagcacc ggccgcccgc
     6001 ggaggtcgcc gacgccgagt atccgttcac cctgatgacc ggacgtctgc tcttccacta
     6061 ccacacccgg acgcagaccg accgcgcgaa gctcctccac cacgaggtgc ccgcagggtt
     6121 cgtccagatc aacagcgacg atgccatgcg gatgaatatc cagaacgggg agaagatcaa
     6181 gctcagcagc aggcgcggtg aggtggagac cctcgcgaaa gtgaccgatg aggtcgcgcc
     6241 cggggtgctg atgatgacga tgcactttgg ggatgcagcc gcgaacaagc tgacgaacac
     6301 cgcgctcgac ccgctctcca agatgccgga gttgaagcac tgtgctgtga aggttgagaa
     6361 gatcacgggg gtgcagtaag atggcagcaa agggagatat ggtatatgcc tggacaacga
     6421 gccccgatat cgccaaagtg gcggagtgcg gcggcgcggt gaccgggctt ctcaagttcg
     6481 ccctggagaa caagatcgtc gacgccgtgc tggcggtgaa gaagggcgtg gacctctacg
     6541 atgcggttcc gacagtcatc accgaccccg ccgagatcgg acagacggca ggctcgctcc
     6601 actgcgggac gctcctgctc tcgaagctga tcaagaagta cctcgatggc gccaatgaca
     6661 tgcgccttgg ggtgacggtg aagggttgcg acgcgatggg catctacgaa ctcgcgaagc
     6721 gcaaccaggt caacctggac aacctcctcc tgatcggcgt caactgcggt ggctcggtga
     6781 gccccgtggc cgcccggagg atgatcgccg cgaagttcgg ggtcaaccct gacgacgtcg
     6841 tcaaggaaga gatcgacaag ggccagttca tcatccagac gaaggatggt cagcacaagg
     6901 gcatctcgat ggacgagctc gaggaagaag ggttcggccg ccggagcaac tgccgccgct
     6961 gcaagatgaa ggtcccccgc caggcggacc tcgcctgcgg caactggggg gtcatcggcg
     7021 agaaggccgg caaggccacg ttcgtcgagg tctgctccga aaaaggtgca aacctcctcg
     7081 atgcggccgt gaaagccgga gcgatcgcct ccgagcccgc gaacccgaag ggtatcgaga
     7141 tccgcggcaa ggtcgagaac gcgatgctga agctcggcga caagtggcgg gcgaaagact
     7201 tcgcggcgct cggcgagggg aaagaacgcc tccagaagat gatggaggac gcgtcccggt
     7261 gcatcaagtg ctacgcctgc atcgagaact gccccatctg ctactgcgtc gagtgcagca
     7321 cgaagaagac ctacctggtg ccgccgggcg agcttcctgt gccgttcatg ttccacctga
     7381 tccggttcgc gcacatcgcc gactcctgtg tcaactgcgg ccagtgcgag gagaactgcc
     7441 cgatggagat tgcaaactcg ctctacatgc acgccctnng cgctcgtcga ggagaaggcg
     7501 gaacgggagc gactctccgc gaccgggagc gaccagatct tcaacgtgtt cgagtaaacg
     7561 acgctccgca ggagcggagt ttgagaaaac gcgaacacgc aacattgcgc ccggccaatc
     7621 cggccgggcg tgtaacgacc accgaataac atcttgagat ccagaaacac ctcactgcgc
     7681 ccgtgccggg acgcaaaagg tcaggcaatg cccggtggtg cgggagagag cgttgctctc
     7741 cccccgctgc cgggacagcc gcaaaagcat ctcctgcgct cttattcaac atttttgcag
     7801 ggtgttcgcc cgcacaacac gggaatcggc aatcggaagg ctgaactgct gcaaccccct
     7861 acgacaatag cagcacaacc ggcagtccga agggtggtga agccgctcgt atcgccggcc
     7921 gccggagcag ggtctgtggc aacggaggga gcaggatgaa gtttatatgc ccgcttattg
     7981 tcgttcgcga catagaggtt tccagagcct tctatgagaa ggtgctgaac cagaaggttg
     8041 gattcgatct cggcgagaac gtatcgttcg agggcggttt tgcgatacat ctccgttcgc
     8101 acttctcgga tctcatcggc gtcgacgaga gcgatattgt cc
//
LOCUS       DAJB01000209            3162 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_88287, whole genome shotgun sequence.
ACCESSION   DAJB01000209 DAJB01000000
VERSION     DAJB01000209.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_88287"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          587 a          911 c          993 g          669 t
ORIGIN      
        1 atgcccgtgg ggccacaggc ataccggatg aaaatatgta ggtgacccat tttcatacga
       61 aaggtcgaac tcagggacag gcagggtcag gcatcaatct cgtcggggaa gaatgacagc
      121 cgaaataggg cttcaccaga gcccgataag ggaanntggt gcgcaacgta attcggtcgg
      181 atcctctgag accacttatc aggtatatgc tggagcacta ccgccacgct ggagaggttc
      241 ggaatagccc cggcggaacc gacggccctc cggcccttat ggatctaaaa aagaatattg
      301 tacggagatc agatgagccc gagactggag agttcggaga ggattcgctg taccgcccgc
      361 ctggcatcct cgggcacctt gccgcccgtg atgatcagct tgccggaccc gaagaggagg
      421 acgaccacct tcggatcgtc gagccggtag accagcccgg ggaactgttc gggctcgtac
      481 tcgatcttgt ccaggttaaa gccgacggca atcttattta agttgatcgg cgtcccgaga
      541 tctgcggatg tgacgatatt ctggatcttg taggtcgggt tgtcggggat atcgatgtcg
      601 agcgcccgca actggtcggc gaggatctcc aggccccggg agaggctgtc gatgctcttt
      661 gccccggtca ggacgacctt gccggaactg aagacgagcg cagcgatctt cgggtcctgc
      721 atccggagga cgacgccggg gaaccgcttt ttattgtatt ccgcgtcctt caaccgggag
      781 gcaagagagg ggagatcaag cgaatcggtc actttcgctg aagcaacgat gttttcgatc
      841 ttgagggact cctcgggttt atgctcattc atatttataa gatgcttaaa aaagtatata
      901 aaaagttggt tgtttattct tccagggtcg atatgtcacc ggggtccata cccagttcct
      961 gcgcttttaa gacccgtctc atgatcttgc cgctccgggt tttggggagc gactgcacga
     1021 attcaatctc ggacgggatg gcaatcggcc cgagcgtcat ccggacatgg tagacgaggt
     1081 cgttcttcag tttctcacag ggctcgcggc tgttcctgag gataacgaag gccttgatgg
     1141 agttcccttt cagggcgtcg ggtttgccga tgacggcagc ctccgcaacc gcctcgtggg
     1201 agacgagcgc gctctcgacc tccgcggtcc cgatgttgtg cccggcgacg acgatcaggt
     1261 cgtcggaccg cccgatgacc atgatgtagc cgtcctcgcc cttcaccgca aggtcggccg
     1321 ccgtgtagca tcccgggatg gtgtgccagt acttgcggta gcgatcgtcg tcgttccaga
     1381 tcgtccgcat catcgacggc cagggctccc tgatgaccag gaggccgccg gtgcccgggg
     1441 cgaccgggtt gcctgccatg tcgacgacgt ccgcgacgac gcccgggatc ggttttccgg
     1501 caaacccggg gcgcatgggt tcgccgatca tcgtggtcac catgtgcatc ccggtctcgg
     1561 tctgccacca ggtgtccacg atcgggcacc ggtcttttcc gatggtgcgg tagaaccatt
     1621 cgaacgcctc cgggttgagc ggttcaccga ccgacccgag gatacggagc gacgagaggt
     1681 tgtagcggtt cggccactct tcacccaccc gcatgaacat ccggatggcg gtcggggcgg
     1741 tgtagaagat ggtgacgccg tactcctcga tgaggttcca gtaggtgccc gggtccgggt
     1801 agtcgggggt tgcctccgcg atgaggcagg tggcgccgtt caggagcggg ccgtagacgc
     1861 tgtaactgtg gccggtgatc cagccggggt cggcggtgca ccagtagatg tcgttgtcct
     1921 tgatatcgaa gacgtgcttc gtcgtataat aggtcccgac catgtagccg ccgcaggcgt
     1981 gcacgatgcc cttcggggac ccggtggtgc cgctcgtata gaggatgaag agcggatcct
     2041 cggcgtccat cgcctcagcc gggcattccc ggtccacacc caccatcagg tcgtagtagt
     2101 ccacctccat ctcggggtgg agttccacgg gcttgtcgtc gtcgcgccgg aggatgacga
     2161 tccgctcgac ggagggcgcg ttgacgaccg cctcctcgac gatgttcttg agcgggattg
     2221 ccttgccgcg ccggtaggtg acgtcggcgg tgacgaccac cttcgcctcg atgcccgtga
     2281 tacgctggtt gagcgcgctg atgccgaacc cgccgaagac gaccgagtgg atcgccccga
     2341 tgcgggcgca ggcgagcatc gcgatgatct gctcgggcac caccggcatg tagatgcaga
     2401 cccggtcgcc cttcccgacg ccgagacttt ttaagccgtt tgcgaaccgg cagaccgcct
     2461 ggaagagctg gcggtaggtg aagatccgct cctcctcctc ggtctctccc cgccacatga
     2521 tcgcgacctt gttccgccgc tggttgtgga cgtggcggtc caggcagttg tgggtgatgt
     2581 tgagtttggc gttgacgaac cacttcgcgt acgggaactc ccactccttc acccggtccc
     2641 agggctggaa ccactcaagc tctctggcga tgctgtccca gaacccgtcg gggtctgcca
     2701 ggaactcctg gtaggtccgg ttgtagtcgc ctatccagga gtgctcttta aagctggcct
     2761 cgggagtgta gtacttcgcc tcctcgagtt tgacgttaaa atcttcagcc atgcgattgc
     2821 cccttattat acattattaa taatgtattt tatatcaaca ttctggttga aggcgtggat
     2881 ccccccgggg ggtatccgcg gggaatagat accggaaccg taccggcgga ggcggcggct
     2941 acggcggagt gtgcacatcc cggcgatctg cggcgaataa gcgtccgcgg agcctgctct
     3001 gggggtggtg tcgatatggg gaatgagcgg acattgggtg cggcacttca ttcaatcggg
     3061 ttgcctgtct tctgacggac tgcctcagcc tttcgcacgg cacgcttcac cacgaaacag
     3121 ggcattccgg cagagagcat acaagatacc ctgcaccccc cc
//
LOCUS       DAJB01000210            7253 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_83791, whole genome shotgun sequence.
ACCESSION   DAJB01000210 DAJB01000000
VERSION     DAJB01000210.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_83791"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1170 a         2047 c         2643 g         1390 t
ORIGIN      
        1 cgttgccgtt caaatcccac acgccatcgg cgcggccgtt gaggctccag gaggtcgggc
       61 cgctgccagt caggcatcgg cacttctcgt gggtcgcgtc gccggcgagc accgggtcgg
      121 taaggccttc gtactcgatc gcgcgcgggt cgccgatgtc cttgccgtag tcattgttgc
      181 ccttgggata ggacaacccg tggcggtagc ggaacgtcat cgcccaggct gcgagcgaaa
      241 accattcgta cggcgtgatc atgtggtggc cgatgatggt gtaggtgtcg tcggtggtga
      301 tggtcgccgg gagctccggg tagatccgga cgtacttggc ctcgccgaac atgccggact
      361 tgatgatcct gcggtagtag gtcgtgccac cctggacgat ctcgacgtgc cggcccacca
      421 gatgagtgat gctgtcgaca aggaactcag ccttcgggtg gtctccggtc gcgtacatcg
      481 cgcaggcggt cggggtgcca gagtcggatt tgtttgcgat tccgcctcgg ttctcgagca
      541 cggtcctggc agtggtccac gagacgtcgg tccacgggac gacgtggggc atgctcgcgg
      601 caccgacgcc ggcaccgggg ctgttcggcg tcaggccccc gcgcgaggtt gctgtggccg
      661 atggcatgca agcctggtac ttatcgaccc agaagccgcc aaggagcagg ccattgaggt
      721 tcggctgcga gaagccggcg ctcctgaaga gggggaccca cacctggtgg atcttcaggt
      781 cgtttggggt gccagcaccg tcctggatct ggagcacgcg gtttaggagc gccggcgagc
      841 gcaggttgag gtcgtaggtc gggtcaccga tctgcgaccc catgacattg acgagcgcag
      901 cgtcaatttt gtctgcattg ctgttgagtg gaacgtccca gtcgagggtg ccggccgcgg
      961 gtttttcgag ggtgaatttc ggagttgtca tgcaaaatca cgtcctagag aagatagtga
     1021 cgatgccctt cgtctgttcg ccggcctcgt catactcttc gacgatgtct ccgaccggca
     1081 cggtgcggac agtcgtgctg ccgttgaaga agatgtaggt ctcgtcttgg atgacatagc
     1141 ggtccgcgtc gaggacctcc cggccggcca gggtatcaat ctggatctgc tgcatcaggc
     1201 agccccccgt gcaaacgagg ccttgacccg gaacgccccg ccgacggtcg cgtcgaagct
     1261 gagcgggttc ctggtctcac ggtagtagag gacgcctccg gacgctgcgg agagtagccc
     1321 gatctccgtg atgactgcgc tgccgagttc tccgggagca aaggtcactt caaaggtcac
     1381 ctggccgccg ctgaccgtgt atgtggcggc cttccgggcg acttcacctt tcatcgtagt
     1441 gtcgccgacg accgggttga cggtgctggt cccaacggcg atccaaccca tcgggctgcc
     1501 agaccctgtc aggagttttg gcagttcgtt gatgccggcg ttagtgacga tcttcccgac
     1561 catcgagcgg cacctcctta ccttctgcgt cgatgcggaa ctggattacc tccgattcca
     1621 cggaaacgca tacattcgcg tcagacataa cgcatgcatt cgcgtcagac atgcaggaag
     1681 ggtcctcctg accctcataa aaaggaaagt cgggatgtta gaacccgaac tcgtactgac
     1741 catacttcca gccggcatag ccgaggtagc tggcggcctg agccatggcc gcggcggtcg
     1801 gctcgatgac caggttgacc gcgctctctg cccggacgtc gacggacccg gcccgggtcc
     1861 cgtagggttc ggtgccgagc cggccggcac cgaagccgag cagggtttcc acgaggacct
     1921 gaaggggttc ggtctcggcc gaggcctcga cggcggcttc gaccgggacg atatactcga
     1981 tcgtggcggc gtcgacccgg ccgccgaacc ctgcccatcc atagggtgac cggccgggct
     2041 cgagcccgtg ccggatgccg gctaccactg ctgcggtcgg ctcgacgacc aggtctaccg
     2101 ccccttctgc cgtcgcccgg accgcgtgca cgcagtaacc gtaactgccg gcaccgtaac
     2161 cgtccgcgcc gaagcccgaa cgcaggggag ttgggactac ccagaggacc tccccctccg
     2221 cgtcgctgaa gacttcctgc agggtgctgc ggaggatgat gtcctggaag tagacgcccg
     2281 cagctttgac ccgttcgatg ccggtaatga gagcagggag ggatatcgag ccaggatcgg
     2341 cgaggagttc gacgcggaac gcggcgtgcg gggtgcccca gggcaaggct ccgtaccgtc
     2401 cttctccgaa tccccatcct tcgtggacgt cgggaggttc ccagatccgg acctggtctc
     2461 ccaaaaggcc ggtggagtat tcgatgacgc cccggatgtc cgcgagggtc ccgcaactgt
     2521 agcactgctt gatccgggtc gtgatgcgtg accggaggtt gtcgtccgac tcgccggagg
     2581 tacgggcgat gttgaacatc tgcgcaacgt agtccagagt ccgcccggcc gtgaggtcgg
     2641 ggtttcgcac ggtgtcgatc gcagcggccg tcgagcgggc agtggcgagt tctccggcaa
     2701 gggtttctgc gacccgggca ttgttgcccc ctggggtcag ggcactggag aggcaggaga
     2761 ggatccggtc ggcggggtcc atggcttaca ccaccgtgat cgagatgagt ccaggagagg
     2821 ctttctgccc ggctgggatg atcagggtct gcccgaactg ctggatggtc gtggacccgg
     2881 cggtagcgga cattgagtgg atatcgttga taccgtcgac accgatgacc gtccggacca
     2941 ggtcggagta gatgacgtcg tccccgatcg ggagactgtc cagatacgcc gtgacggcgg
     3001 cctccaccct atcctggaca gtgggggcgt ctactgaggg gattcgggat acggtcgcgg
     3061 tgacggcgac gctgaccggg gtgggccgct gccaggtggc caggattcct gccggccggg
     3121 tatcttcgag tgtggcggcg atctcggcgg cgtccccgcc ggagacggtg agggtgatcg
     3181 tgcagtcggt gaagttctcg tcgagggcaa cgtcctgaac cccctccagg gcagtgagtg
     3241 cggccacgat gctgtaccgc gtccctttcg cgaaaggcga gtaggagatt gtgcggtagc
     3301 ggagctcgga gtcggtctct gtgtccgctc cgcctgaggt ggcgtccgtg ttggtgacgg
     3361 atgtgactcc ggagataggg tcgacgagcc gggtgatcgt ggccggcgcg acgttgccgg
     3421 cggcgcccgt gtcgacggcg atcaccggga ccgagacgga gaggccgatc aggtcgaccg
     3481 tctgcgtggt ctggtagacg agggccaggt cctgggtggc cacccgggtg ccggtcggga
     3541 tggtgatgat cgacccggca gccgaacgcg agaagaggat ggtgccttct gcccgggtgg
     3601 cgggcttgcg gaggactccc agcagtgcga cgagatggtc cagactgtcc ccggtggcaa
     3661 agtcgatgta accgctgtag tagatgtcct cgaggatgtc ccagatgagg gcattctcgt
     3721 aggcgacgga ctcgagcatc tgatagaacg gggtgtgcgg ctggaggtca atatcgtcgc
     3781 cgagagtcgc ccgggcccgg gcctcgtagt ctgccaggat aacggtgaac ggttttcgta
     3841 cgaaccctga aggggtgacg ccgtagtcgg tcatacatca gcctccagag tgacagtatc
     3901 gccgtcgtgg agggtgacgg tggccgtgat cgccgcgccc cgggcaggga tgtcccggct
     3961 gatctccagg aagtcgacct gcttgaccgc aggatgggag aggagggcgg tccggatctc
     4021 ggcgttgatg agggtccggt tatatccgct gtgggcgatg gccacccagt ccacgccgaa
     4081 cgcggtgttg aaggggtagg accctttgac ggtccggagg atgatcgtca ggtcctgggc
     4141 aaccttggcg gtgccggtaa tctcctcgaa ccggttgagc gggcttttga gcaggtcccc
     4201 gtcggaggtg atctgcagcg tcctgccgta tgacatgata gggggtcggt gatgcgagat
     4261 aaaaagagat gtcaggagtg atcggtgacg aaggcctgcc cggtgacgac gatcccctga
     4321 ccatcgtgat cgccgtcctc cccgacgtcg cccgcccggc agacagggac gccgttgatc
     4381 cggacgagcg ggcttgctcc ggtggtgccg gcggtgcagg tggcgtcgca gatcgggcag
     4441 tcggtcggga actcctggcc ctgcaggatg gctggcttgc cggcaatccg gacgagggtc
     4501 tgcagggtgc cgggaatggt cttgtcccca ccaccggtcg gggggatgtg ggcgtctgag
     4561 taaggctcat agtctccgtc gacggcgatg aatggcatgg tcagagctcc acgaagtcga
     4621 cggacgttgc ggtgatggtg acgacacctt caccggtgat cgtgatgccg gccccggtat
     4681 ggtgggtgat ctcgatatcg ccggtctgcc ggatccggta ctcggtgccg gagacgtggt
     4741 ggaggaggat ctcgtcttcg ccgacggcgg ggggggtgtc ggcgagggtg tagagaccgg
     4801 cgacgacgac gacgttgttg atagagaact ggtggcgctc gttgaccggg acgacgtcgc
     4861 gggtcttgag ctgctcctcg aggtcttgct tggtgaacgc gacgaggacg atatcgccca
     4921 ccttcggcgc gacgatgagg gccgagccgt ggtaggcctg gacggcgatc gggacctcga
     4981 agagctcgac ctgctggccc tgcaggaggc ccttgaggag gacgttgcac ctcagccggg
     5041 ggaggtcgac ctgggttagg atcccgaccc ggcaggtgtt gatcttgtcg acggcccggg
     5101 ttgcggcggc gacgatggcc tggccgacgt tcatatgggg accacctcac actcggtgct
     5161 gtagtcatcg ccggcaagcc ggtgcgtgaa ggtcttgacc ttgaagttgc cctggacgac
     5221 gcgggagtct agctggacga gggagtcggc cttgatcttc cagttgagga tgcacttcac
     5281 agtgtaagcc tcgccttcct ggtcgtcttc ctgcgggaca acttcgagga gcccggtctc
     5341 ggaggagagg atgacggcgt cggtctcgct gtgagcctgc cggacaaagt agccggcgcc
     5401 ggcactgaca aagtaggtcc agccctgcgt ggcgatctgc cgcttgataa gaccggtgag
     5461 gtcatctccg ccagccgcag tggtcccgat gacctcgtct ccgttgacga tgtccaggca
     5521 gtcgtcgagg ttctcctgcg gggtgccggc gaaggtgtag ggtttcgcga gggtgatgcc
     5581 cggatcgtcg acagtgccga cggggatccc ggcggcggcg aactggtcca ggatgacggt
     5641 gacgagtgcg gcgccggcgg ggtactgccg gacgatccgg gtgccgaaat acaggttccg
     5701 gacgccgtcc tggaccgtga tggcggtctt gacgtcggca ccctgccgag aatcgtagat
     5761 gcggtcgatg gtaccgagga agatgacgcc atggtccgcc cggtagccgg cggtgagctg
     5821 ggcggtctcg ccctttttga tgcgggcctt ggtggcggcg gcgaggttcc agatcccgat
     5881 ctcggccagg tccgcctcgg tgctgttgct gccctcgacg ctgaactcga tgtcgagttc
     5941 atcgatgctg atctggaggt cgccggtctg gaaagcgtag tagcggtccc agagctcagt
     6001 catagaagac ccacacctcc gctttcgtgg tgtcgacctg gtggggcatg atggtgaaga
     6061 ggagcgcccg ggtgacgggg tcgcggatct cgtagccctg gagctggaca agcttgcccc
     6121 gccagatgac gtgctcgtcg gtgctccgga ggacggtcag gacgcagaac ccgccggcgt
     6181 ggttccaccg gtagatgagg tcatactgct gctggtcgat gaggatccgc tgccgctgcg
     6241 ggtatttgag gaccgggtcg aacgggagga cgcggaccat tatttcccgc ctccaaagag
     6301 cccgccgacc cagctgacga tgccgtcgag ccagctgccg ccggacttct cgggttcgct
     6361 ggcgacgggc ttctcgggcg gggtgtacca gctgccgggt ttggggggtt ctctgccggt
     6421 gtcgataggg attgggaggg cgacctggac ggttttggca gtggctttcc ggacctgttt
     6481 gagagtgaga gtggcgtaga cgatgttgtc actgtcgttg tccttgtacg ttggttgttt
     6541 gacggccatg tcggcgaagt gacccaggcg ggaggtgacg tcaatgagct ggcgggcnnn
     6601 tcggcgacgc tgaactggtc ctcgacgcgg tgttcgggga tgtcggcgtc tttgctgagg
     6661 ttgatgacct ggatcgccct gaactcgtgg ccgccgatta ggacggactg gtctggcatg
     6721 ggtagggggt cgctactgcg agataaaaag agaggtcaga tcccgaacgc ccggaggtgg
     6781 tcgacgagcc ggcgctcgat gatcttgacc tggtcgtggg tggagcggga aagggcgtcc
     6841 cggatctgtt tctgaatgtc gctgttctct gttccgttga cctcgatggt gttctggatg
     6901 gtgatggtga tgggggcggc ggtccgggga gtctggcccg gggtgcccgg cgcctcgatg
     6961 gcggggatct ccgggggtgt gatggccggt atctcggggg cggcgatagc ggggatgcgg
     7021 ggagccacga ggtccgggat cctgggagct gcgatagcct gcacttccgg gggcgtgatg
     7081 gctggtatct ccggtgcggc aatgtccggg atgcgggggg ccaccagatc ggggattcgc
     7141 ggggctctga gggccggcat ctccggagca agcatctgca cggcgggggc cgcgaaggcc
     7201 gggaggatgg gagcggcgag ggcgccggcg aggagcgagc tgatccggtc gag
//
LOCUS       DAJB01000211           10757 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_88727, whole genome shotgun sequence.
ACCESSION   DAJB01000211 DAJB01000000
VERSION     DAJB01000211.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10757
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_88727"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2057 a         3399 c         3128 g         2173 t
ORIGIN      
        1 tagcgcccgc ccccacacgc caaaattcct aacataaact gacaagatgc actagggtgg
       61 tggaagggag ccgtgctcca ggctgcagcc cccctccccg gcgggctccc cgcacccata
      121 ccctttataa gcagaagcga caaggctggg ttcaaacagg ggaataacca tgtcagccag
      181 attggtggat tacttcaaca aacagccgag aatcggtatc ctgagcaccg caaacagaga
      241 aggaaaggtc gatgcggcag tcttcggctc gccgatgatg atcgacgaga agaccgtcgt
      301 catgggcctt gggcagaatc gcacgtttac atacctgcag gagaacccga acgccgtctt
      361 catgatcgtg gagcagggag aggggcttat ggactggaag gggctccggg tctacctgaa
      421 gatgaaggat tatgcgacct ccggcgagac actggagacc tacaagaaac aggtcgccag
      481 agccacgggt gaggatgcgg cggcgatgat ccacgccacg gtgacgttcg aggtcactga
      541 agtgcgaccc ctcatcgata tgggtcaggg ctgggagaaa tccatctgat tttttagggg
      601 agggcgcccg ggcgagccct ctcccgccta ctctgagagg aacggaaata tccgccgcaa
      661 gttctcgttg agcggcttct tcttctgcca ggtctcttcg agcggcgtgc acgcgaggtc
      721 accgccgacc tctcccacca tgcacccgtt ccggccctcg atcagcgcct cgacggcggc
      781 gacaccgagg aggctcccga gcacccggtc gcgcagcgtc ggcggtccgc cccgctggag
      841 gtgcccgagg acgacgacgc gggattcgaa gttgaggttc tccccgacct ccctggcgat
      901 tgaaaaggag atgcccggcg tctccccctc cgccaccacc acgatcgcgc tcttcttccc
      961 gatggaaaac ccctcccgta tgcgctcgct gatccgggtg atcgagacct cttcttccgg
     1021 gatgaccagt tcctcggcgc cgccggcgat gccgctctcg agcgccagga acccggccgt
     1081 ccgccccatt acctcgatga agaagaggcg ctcgtgggac cgggccgtat cgcggattcg
     1141 gtcgatcgcc tcgactgcgc aatttgccgc cgtatcgaac ccgatgcagt agtcggtccc
     1201 gtagacgtcg ttgtcgatgc tccccggcac ccccacgagc gccgccgtat ctgcatcggc
     1261 cgcaagcagg cttgcgccgt ggaacgtccc ctcgcccccg atcaggacga tgccgtcaag
     1321 ccccatccgg tcgatggccg ccgctgcctt gagtctccct tcccgggtgt agaactccgg
     1381 gttccgggag gtctcgagga tggtgccgcc caggtgaatg gtgtttctga tcgccgcccg
     1441 gtcgagcggc acggcgtcgc cggcgatgag cccggtatac ccccggcgta tccctacgat
     1501 ggtgaggtcg ttagcgagcg cggtccgcac cgccgccctg atgcaggcgt tcatccccgg
     1561 ggcgtcgccg ccgctcgtca ggatgccgat gcgcctcatg gggtccctgc atcatccagc
     1621 gacgtgacgc ccggaagcgg ttttcctgca agcatcgtca gcgcggcccc gccgcccgtc
     1681 gaaacatggg tcatctggtc ggcaagcccg aaccgttgca ccgcatccgt ggtcgagccg
     1741 ccgccgatga ccgtggtgcc gcggaggttg gcgaggctcg cggcgacccg gcgggtcccc
     1801 tctgcaaact ccgggatctc aaagaccccc atcgggccgt tccagacgac ggtccgcgca
     1861 ccgccgagtt cccgggagaa ctcgtcggca gtcccgggcc cgatatcggc gatgacccag
     1921 ccgtccggga tctctgttgc agatacgacc cggaccggga cacccgcttc cagcctctcc
     1981 gcgacgacga cgtcccgggg caggaggagg cggacaccaa gttcagtcgc cctttcctca
     2041 agttttctga cggcgtccag gcggtcggtc tctacgtgcg atgcgccggt cccgtacccc
     2101 cggctcgcaa ggaacgttgc cgccatgcct cccccgatga tgagtccgtc cacccgggat
     2161 atgatgttat cgaggacctc cagtttgtcg ctcaccttgg ccccgccgat cacggcggca
     2221 aacggcctct cggggttctc aaggatgcgc gtaaaggcgt ctatctcctt ctccagcagg
     2281 agcccggcca ccgccggcag gtgctccggg acgcccacga ccgatgcatg ggctcggtgg
     2341 gatgccccga aggcatcgtt gacgtagatc tccgcgaggc cggcgaggct ctccgcaaag
     2401 gccgggtcgc cggccctctc ctccgggtgg aaccggaggt tctcgaggag aacgatatcc
     2461 ccgtcgttca ttgcggctac cgcgctctcc acctccggtc cgatacagtc ccggagcgcg
     2521 acgaccggcc gggcgagcag cacggagagc cggtttgcca caagggcgag gcgtaaccgc
     2581 tcttcgacca cgcctttggg ccggtccagg tgggagcaga ggatgacccg tgcgtcccgt
     2641 tcacagaggt agcgtatcgt cggcaggctg gcccggatgc gggtgtcatc agcaatagcg
     2701 ccgtcctcgt cgagcgggac gttgaagtcg gcgcggacga gaacccgctt acccctcacg
     2761 tcgatctccc ggatggtctt tttccgtctt agtatcacgt tctgcatacc ggcatacctc
     2821 cggatcagcg gggacgggca ggacttctgc gccggtgggt gtacccttcg tccggcttcc
     2881 tgctgtcttg ccgttccccg cggaattacg gtctcccgtg catgcgtgcc ctggcatata
     2941 tagtgttcgc cgggtgcaac cgggcttgcc agggttccaa aaactgtctc cggccacggg
     3001 cccggctctg cacacgggtc cgaagccgac ctgatgcctg cttttccgga gagttcaccg
     3061 ctcccggcgg cgctcccagt agggtgcata tccgtagtgg ctgtacatgc tggtgagcca
     3121 ctcacgctcg gcggtcatcg gccagttgtc cttgtcgaac cccggtgcgt tatcgagccg
     3181 ttctttaggc acgtcaagga cgaaggagtc atcggtcgcg ttgtaccgaa gagcctccca
     3241 ggggatggcg aagagcttgt cgccgagtcc catgaacccc ccgaacgaga gagcggcgta
     3301 ggcaatgcag ccttcgctgg tatcgatcac gacgtccttg atggtcccga ggtcctcgcc
     3361 ctgcgggttc ctgaccgaat agtccgcaag atcctttccc cgtacaatac tccgtgtccc
     3421 catctctgtg gtagctcctc tcatctgcat cacatctcct ggattgtgga acctgctgtc
     3481 ccacggcaag agagtcaata gaggccgggc agtatttaat tcttgtgaca atataataac
     3541 ctggacatag aaaaatggcg gccgctctcc ggcgatgccg cctccggctg cctatcccgg
     3601 agcacaacct gccgcttcgc ctcttccttc ctccgttcac tacccggtcc cttctcgtag
     3661 acccgctcca ccgccgcccg ggtgaccttg tgggcaacct cctcacgtga ctcttccggg
     3721 ttttgccgct ccgaagggtc ggcatactgt tcccgggcac tgttgaacgc ttcacggtag
     3781 atatcccggt cgtgctcggg aagcagatcc ctcaccagtg gggcaggtct tcgttactcg
     3841 catagtatgg catatgttct cacccactca ggtgcgcccc gggtgtatcg aacagtataa
     3901 aaagattcag gtgtccgtag gcgaaccgga ccggcgtgaa gtatcatcgg ccgaaccggg
     3961 cctgagaggt gagaatcctg cgcggggagg gggcaggacc ccgaaccccg catcgtctcc
     4021 caggcgatag tgcttcttca gaaattgcct gacgtttaac gatgcgacgt acccctggtc
     4081 cagttccgtg atgatcccgt cgatgatccg tgcctgttcg gcgatctttt gcgtgacctc
     4141 gttgtcctgc agatcagcaa gaccaacgat gacggcgagc gggttcctga tacgatcgcc
     4201 gaggcatgcg aggtgctcaa tgttttggtc gagttgggaa caggtttccc gccacagatc
     4261 gtcgtcggtc ttcggatgcg caaccgcgat gagcccggtg aggctgcctt caccatcccc
     4321 tacatgcgaa aatccgaaga ttgcggcaat cggttgattt cgcgcgtcga taaggttcac
     4381 ctccatcgtg cgcgcgccct cgaccggatc agaacgcccc gccgttcctc tggagacgcg
     4441 atcccggaaa caggccttct ggaattcggc aatagaatca ggggccatta tggaagagag
     4501 gtgttttccc gtgagatcgt ccgggttatg cccgaggagg cgccctgccg aggggctcac
     4561 cgatagtatc gtaaggccgg gatcgaggga tatgaccggg tcggaagaga tggctgccag
     4621 gaaggcatac tgctgctggt attcccaaag actcgtctcc agccgaccgg aatccgggct
     4681 ggcgtggtag gtatccaggc gcgccccctg gttcggtccg tcggggatca cggtgctcga
     4741 gtactccacc cagacccttt tcgtcggagt acgggatatg cagtagcgcc tggctggaat
     4801 attttcacag aagaagcagg atacgatgaa atcgtctttc aaatcttcgc cgtccaggag
     4861 gctcggggag atgcagaggg agatgaactc cacggcactc atgccgatcg tttcgtatcc
     4921 gctcactccg aagagcccct ccatagcctt gttaatcccg gcgaccgtct gatccgggcc
     4981 gatcagcacg attccatcct cctggcagtc gaccagcagc gccaggaggt cccgggcaga
     5041 tgccgccgta acgcgggatt cgccctgcgc gccgcaactg ccgggacaaa gcagcatctc
     5101 ggctacccgc tctcgttccg acttctgctt tccagtcgtg ttgcaattca tacccgctgc
     5161 cccggagtaa tgcacccgtc tccccgggat atactttaaa ataaccgata taaatggttt
     5221 tcttacggtt ccggtgaaaa aatacaggta ttttggaaaa ttacagccac cttgtgcctg
     5281 tacaatggta ctgtacaatt tcggtcagaa tggaatattt tgctgtcccg gattgtgtac
     5341 accatgcaag cgcaacatct tatcatctcg atatttgtag ttacgctttc aggagccctc
     5401 aaactgcaat tcgcgtatgg gttcctcgga agccatgtac cgatcaccga atttgccgcc
     5461 gccgcactca tcgcatacgc agcctatgcg ttcgaccgcg gcgtggagaa taaggaggat
     5521 gagaaacggg gaagcgggtt taaaaaagcg ctcctgttga cagcactcat cgccatggtc
     5581 ggcacattcg ttctctatcc gaaccccgcc ctcgtcatac cgttcctcgt tgcgtacctg
     5641 tatgcgaagg gcatcggggg ttatcggttg aaggggggca gcgggacaaa aaactgcatc
     5701 gtcgctctca cctggtcgct tggtatcctg atcttccttg gcgccgtcac cctgccggtc
     5761 gtgctggtct gcctcttctt cttctgcaaa agttttgtca ataccgtcat ctacgacgtc
     5821 cgcgatgtcg ataaggatag aatggcgggt atcctgacga tcccaaccat tctgacccgg
     5881 gtacaggtca cctccctgct gctcggtctc agccttgttg cccacgtggt agtctttggt
     5941 gcatatttca caggccttat cgccggcatc gacgttctgc tcatcagttc ggttcacagc
     6001 accttctaca tcatcgccta ctgcaacagg tttgagatgt ttcggaacgc gctcgtggac
     6061 ggggaatgga tcctctatac cggttataca atcctgcggg attgcatcct gtaatggaga
     6121 atgagatcct taccaaattt tattgcttcg ggagacgttg cgacgaggtc ccgtgtgaag
     6181 tcgtaacttc ctgacttgta gaagaacccg atggagaaga aggtgtccgt gacggtggca
     6241 gcgatcctgg gggattctgg agtgaggtat atctcaatat tgtcgtagtt cccgagcggg
     6301 taggtcgaga tgatatcgaa gatcccctgc gtgaccacca gcgatatcgt ctcaacggtc
     6361 tctgccaggg tcttgaagga ctcggtgtaa tcgggaaaga gaatcgggga gacgccgtat
     6421 atctcccggg tattctttag attgtcggta aatacccggt gtggcttcaa gatgtcggac
     6481 ggttccgagg cgataacccc ggcatcgcgg atctcacaga gacggagagc gaactcgtcg
     6541 gggaaagcaa tgagatggtc cgggaagact tcgtagagca tccggagggt ttcgatgctc
     6601 tccttgaacc gcttgaagac gacgaggttg gcgtgccctc tatcggtcag cgagacctcg
     6661 ccgtcagcct ccctgacaaa acccaccttc ttgaggtgat tgacggtaaa agcataggtc
     6721 gaggacggaa tatgctcctt gatctcatct ttggactctt gccgcagaag cgcatccaga
     6781 acctcaattc ggcgctcgct cgaaaagatc ttcatgaaat cggtgatctt catgtccttc
     6841 tagtatttcg tcggcagcgt cagattaatc ttcttctttg aagagatggt atactgaaat
     6901 actctgcgga accggagaac gatcggttcc gctggagact cagcaccggg accgctcctg
     6961 gccggtcccg atctctcgac gggagagcag acgaggatct ttcttacacc catgaacgtc
     7021 aactggcgag ccataccccc ggcaaacaat tccctggccg tcctctccgc ggcctgcgag
     7081 ggtaacggat accccctcga gatcaccgac gaaccgcttc ctgacatcac ctgctacagt
     7141 ctgaactcca tcaacgaacg tttctaccgc gacgagatcg cgggagccga ctgcataacg
     7201 attgtcggag ggccccacgc ttccgcctgc taccgtgagg tggcggagta tgccgactac
     7261 gtcgtcgtcg gggaaggcga gtacacgctc ccggcactcc ttgccgccat tgaagagggg
     7321 agggatccgc ctcccggcgt cgccactgcc gctggctaca cgcccgcccg ccacaccgtc
     7381 ctccttgacg gctacccacc cttctcggag atcaagggtt tcatcgagat cacccgggga
     7441 tgcccgttct cctgcggata ctgtcagacg ccccgcctct tcgggcggtg catgcgtcat
     7501 cgctccgtcg acgagatcgc ccggtacgcc gcgcggttcc gcgatatccg ttttgtcagc
     7561 ccgaacgcgt ttgcttacgg ctcggatggc atccaccccc ggctcgataa ggtggagcgg
     7621 ctcctcaaga gccttgacgg ccggatttac tttggcacct tccccggtga ggtgcgcccg
     7681 gaatgcgtca cgcggcagtc cgtcgagctc atcctcgacc actgcgccaa cacccgcctg
     7741 cacttcgggg cccagtcggg gagcgatgcg gtgctccgcc gcctgcaccg ggggcatacc
     7801 gtggaggacg tcatccgtgc cgttgacctc tgccgggagc aagggctcgt cccggtcgtt
     7861 gatgttatcc ttggtctgcc gttcgagggt gacgacgacc agcgcgcgac gctcgagctc
     7921 gtgaaactgg ttgcccgggc ggggaaggcg cacatccaca gcttcatgcc gctgcccggg
     7981 accccgctcg caaacagccg cccacggggg ctccttccgg aaacagaaaa ggttcttggc
     8041 aaactggctc tcggcggcag aataacgggc tcatggatgg atcatgagat aaggtttttt
     8101 agacgcactc ctcacctata ggcatgacgg atgtgctact cttagcagat ctgcacggta
     8161 actacggaaa gcttgacgct tttctcagct acgactacga tgcagtcatc attgcaggcg
     8221 acatcaccaa ctttggtcca cttgagcctg tagattcggt actctccaac tttgaagtac
     8281 catgtttcgc aatccccggc aactgcgacc cccgcgagat catcgacgtg cttgaacgct
     8341 cagatgcgat ctgtctgcac aactcgtgga tggccattgg caagataacg ctcgcaggtc
     8401 ttgggggttc gaacaagacc cccttcgaca cgccgtttga gctgaaagaa gaagtgattg
     8461 ataaagaact cacccggatc gttaaccaca tggacaagaa tgtccacaac gtcctgctct
     8521 gccacgcccc gccatacggg gcgctggatc tcgtcggcga gaatcacgtc ggaagtcgga
     8581 gcatcagaaa gcacatgacc cggttcgacc tcgtctgctg cgcccacgtc cacgaggcac
     8641 ggggcatcac cgaagtcgac ggcgtcaccg tcgtcaaccc cggacccgcc tcagaaggct
     8701 acggcgctat catccacttt gggaaggagc caaaagatat tcatatcgac cttatcgctg
     8761 tgtagatagc agcatctcca gatacgaggt gtagatcggt ggaccgtcta cacccaattc
     8821 ttttccaatc acgccgatcc tgtctgcagc atcctctcta tcatcctctg tcaggatctc
     8881 gatctcgatg aacgtcccga gcccctcgac gtcgtcgagc gtcaccgtca catcccccat
     8941 ctcgtagaac tcccgaactt tcgagaccgt ggcggcccgc cggaaaccga gacgggagag
     9001 aatcgtctca agggtttcgc ccgaagccac tgcgaggttg aactcctcgc gggcctttgc
     9061 gctcccgacc cggaccttcg gccctttgta ggtcacggta aacccggtat cgtcgtacct
     9121 gacccttaat gcctcgtcgg tctccccgaa atcccggtgg ggggcgttgt agtagacgtc
     9181 gcgttcctgc tgtctcctgg ccatctgcac ttctttctgg ttgagccggg ccctgacttt
     9241 ttccaggtca gggaccgcaa actttgcttc tacctcgagc atgccaatca cgtcagatcg
     9301 tctctccgga gcagtgataa accctcactg cacggcccgg atcggaccgg cgatcttctg
     9361 tcggatcagg tctctctgct gctctgcggc ggtgcggaga gcgccgtccc cgagatgctc
     9421 cgcagagacg atcgcccgct ctgtcagcat cagagccgta tcgtaatcat cgcggtggta
     9481 gaaccggagc ccgagctcca ggttcaggtc cgccgccttc ccgtactccc gggcgtggaa
     9541 gaagtggcag gcaagtgaga ggaggacgtg cagcctgccg gagaggcggt gcagcgaacg
     9601 ctcgaagcag tccgccgcac gggcgttgag cgccgccttt gcatcgggga agagttcccg
     9661 cctgcagtgc tcctgcagga ggggatgcgc gaaggcgtac tccccgcccg ggagcctccg
     9721 gaatatgccg ctctcctgca tccggtccgc catcagcgtc acgcccgccc cctggaggtc
     9781 gagcatgcag gccatgaccg gcaccgggaa cggggggttt aagaccgaga gttccttcgc
     9841 ccatgccttc cccgacccgg ggagcgtgga gaaggcaagc cctgccggat cctctgctcc
     9901 gtcgagtacg tctgcgatat tctcgggggt aggcgcaaga tcccggtgat gcagggcgtt
     9961 gaagcaggcc gtgagactga acgggtcgcc cgaggtctcc ttgaggagac cggcgactgc
    10021 atcgtcgatg gagaggccga accgtctctc cccccatctc ccggatctcg tgccgctaca
    10081 tcccggagag cggcacttcg gaaaaaccga ggccccggat cccctcctgc atcgatatgt
    10141 accccgaatc ggtcgtattc cccgtccggc aggtgaaggc aaggaggatg ccgggcggga
    10201 ggtcgtgcat gatctcccgc agaaggcggc ggtcaaggct cgaggcgaga tggacatcgt
    10261 cgagcagtac tgccgcaaca cgaccggtcc cctccatcag cctcccgagt tcccggagca
    10321 actcctcgaa cgcctgatat acccggtcat acccgacctc aggcgaccgg gcgaagacac
    10381 cgatgatctc ctcgccggtc ttcgcgagca ggccgaccgg ctccaccggg gcggcatact
    10441 tctcgaagac gtcgtcggca taccggatcg cctccctgat gccctcgata ccgggggtat
    10501 tcccaaacct cgcggaagcg gcagaccgtt gcaggtcttt taccaggtca cgaaacgcag
    10561 agaagatcat ccccggaagg tcaaagacct ccgccctgag gacgggggac tccagcccgc
    10621 cggagcgttc ctgcacttcg tatgcgagcc aggttaagag cgaactcttc ccgatgccgg
    10681 gcgggcccga gatcattacc gcctgcccat gctctcccgc ctgcgagagg atgggtcaac
    10741 tacccccggt taaaacc
//
LOCUS       DAJB01000212            3830 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_66257, whole genome shotgun sequence.
ACCESSION   DAJB01000212 DAJB01000000
VERSION     DAJB01000212.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_66257"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          766 a         1167 c         1193 g          702 t
ORIGIN      
        1 cgtgaccttt gattccccgg agtctcccca caccaggcct ccattcagta gtggccccac
       61 tacaggatat gcatttcgct gccgggagac cgtgccccag tgacgaccgg ctctatcatc
      121 ccgggctctc ccaaccccgt cgcccccgat caaccgtcct taagtagtac gaaacctcaa
      181 tttcaaccag gatggggtta ctcacatgaa actgacagta aaactgctcg acatcgaaaa
      241 ccgtggggtt ctcctccaca gcaccgacgc acggaacatg cgggtccgcg atggcgaccg
      301 cgtccagctc gccgacgagg taacggggca gaccgttcag gcccatgtcg acaccaccgg
      361 gtcgctcatc gaaccgggcg tcatcggcgt ctaccggccg ctgaacgcca cgctcgccgt
      421 cgacgaggga actcccgtcg aggtccgtgg cgctgagagg ccggcatcgc tcgcgcatat
      481 caagaagaag atggacggcg gccgatttac caaggacgag accctggata tcgtcaggga
      541 tatcgtcagc gacaccctct cgccgggcga gataactgct tacgtcaccg catcctacat
      601 caacggcctc gatatggacg aggtggagta cctgacgaga gccacggtcg agaccgggga
      661 acgcctccac ttcacccggc accccgtcgt cgacaagcac tccatcggag gtgtgcccgg
      721 gaacaagatc accctcctga tcgcaccgat catcgccgcg agcggtcttc ggatcccgaa
      781 gaccagctcc cgcgccatca ccggcgccgg cgggaccgca gacctcatgg aagtcctcgc
      841 gcccgtctcg ttcccggcga tcgaggtgca gcagatgacc gagaaggtcg gcggcgtcat
      901 cgtctggggc ggggccacga acatcgctcc cgccgacgac aagatcatca cctacgagta
      961 cccgctccgg atcgacgccc ggggccagat gatcgcgagc gttatggcca agaagttcgc
     1021 cgtcggcgcc gacatcgtgg tcatcgatat cccggtcggc cggaacacca agatcgcgac
     1081 ggtccaggag ggccggaaac tcgccaggga gttcatcgaa ctcggnngag gcgctcgccg
     1141 ttctcgaagg ggcgaccgaa ccgaattccc ttattcagaa gagtctctcg ctcgccggga
     1201 tcgcgcttga gatggccgga aaggccgcgt atggccaggg tgcccaggca gccgccgagc
     1261 tcctccggag cggcaaggcg ctcgagaaga tgcggcagat catcgagatc cagggcggcg
     1321 acccgacggt gaaggccgcg gatctcgtcc ccggggagta ccggttcgac gtgaaggcac
     1381 cgcaggacgg ctacgtcatc gagttgaaca acagcgcgct cgtcacgctc gccaggctcg
     1441 cgggctcacc ctacgaccac ggtgcggggc ttttcatcca cgcaaagaag gggacccggg
     1501 tcaggaaagg cgatcccatc ttcaccatcc acgccgagcg ggaatggcgg cttgagcgcg
     1561 cgatagaggt cggccggacg ctgatgccgg tggtcgtgga agggatggtc atcgagcgca
     1621 tcccacctga acgctgggtg taaaccgatg cactcaaatt ttaggccgca gtacactcca
     1681 gcatgaagca acgtcgtctc gttatagccc ttctcatctc ctgcatcctc ctctgctgta
     1741 cggcgcaggc ggtcaccctg cggatcacgg tggccgacga gaaaaccgat aatgccatct
     1801 ccggcgcctc tatatacatc gacggcacct atgtgggaag cacttcgtca agcggcacct
     1861 actcctacgt ccattccggg tacgatgacc tgtacctgaa ggtcgtcaag acgggctacc
     1921 agaactgggt ggattatgtc gatgacgatg caactcgtgt agaggtcgat atgtcccgcg
     1981 cggacgagac cctcacgttc gaactctacg atgcagggac tctgcagccg atcgtcggtg
     2041 ccgtggtgtg cgtcgagggt gacgactact ccgactcgga ggtcaccggc agcgacggga
     2101 gcgcgaagtt ttcggtggag tcggatgagt catacagtgt tgaggtccgc gcatcgggct
     2161 actacgagct ctcaaagacc gtggagatgg agagcagcga tagggtcgtc cagtactggc
     2221 tctacagcaa cgacctcatg gcgttccagg tcagggacag tgatacttcc gcagttatca
     2281 aaggcgcgga ggtctacata gacaactcgc gcgccggggt gaccgacgcc gacggccggc
     2341 tgcagctcca cctggaacgg gcgaagaggt attctctcag ggttacggcg cccgactacc
     2401 tgacttacca ggaaaaccgc tatttcgaga cggacgatgt ccttctccgg gtggatctct
     2461 caaagtcggc gtatcaactc tcgataacgg ccttcaacga ggcgacgaag ccggtcgagg
     2521 gggctgaagt ctacctcaac gggacgctgc tgggcaagac cagccagtac ggccggttca
     2581 tgctttctga cgtccacgcc ggcacctacg aggttctggt gcgggcctcc ggctacgagg
     2641 actggagcgt gacacgccag atctccgggc agggggagga tattgtcgcg gagctcgatt
     2701 acgaccgggc gaccgtgacc atccgcgcgg aaggtcccga ccgggaagcg gtggtcgacg
     2761 cggtgattgt catcgacggc caggtcgtcg gggtgaccga cggcctggga tacctcagaa
     2821 cagccctcgc tacgaaccag gtgtatgccg tcaccgccgc ccgtgacggc taccggaaca
     2881 tctcggtcga tgccgagatc ccgctcggca cgaccgagtt caccgtcccg ctcgtcatgg
     2941 agcgggactt caacgtctgg gtgctcgttg ccggcgtcgg ggttgttgcc gcggtcatcc
     3001 tcgcggcggt cgcggtcgtg cggcacaggc gggcggggca gaacaagggc aggtcacgcg
     3061 gccgggacag cctgtaggcc tccccgagtt gcttttttta gaatccgggt tccgggcggt
     3121 ataatgttcc cggagtcgga cggcggaact cttccccccg gagaaatgat attttcctga
     3181 tggaggtctg gaaatacctt tctttcgccc tgttatcctc tctgtcaaat atttgttcaa
     3241 tttgcgtgta tattattgcc gatcaggaaa aggagccctc tcagttgccc tcctcactgg
     3301 caacctttat atgtgatggt gtgtcaaggg atagatacgg tagagtatgg gtggatggac
     3361 gtatgcgtgt ctggtagtgg ggctgctcct tctggcggcc ccggccgggg cgctgatacc
     3421 tgataacgtt acaattgtca cggatactcc gtggttgacg gcggggagcg gagataccgc
     3481 gaccatcacg gtacaggtct cgaacagcac ttccggcaac gtctcgttcc cgggggttgc
     3541 tgtcgatttt acagtaaata gtacctatgg cagcatttcg cctgcgcgcg cggtgacgaa
     3601 tggtgaagga tatgctacgg cgatcttcaa gcccgcgacg cttagcggga tcgcgacgat
     3661 cacggcgacg gcgtcgtacg aggggctgga tgagcccctg accggaagcg tcgatcagca
     3721 gatagaccat gcggcaccgt attccgtcgc caatctctgg tacgcttccg aggtgaccgc
     3781 cggggagacg acgccgatca ctgtccggat ggtggaccgc tacgggaacg
//
LOCUS       DAJB01000213            7922 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_109208, whole genome shotgun sequence.
ACCESSION   DAJB01000213 DAJB01000000
VERSION     DAJB01000213.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_109208"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2787..2832
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1446 a         2380 c         2571 g         1477 t
ORIGIN      
        1 caaccgcttt tccgtgcagg aactccacca ctgcccgggc gatcgagcac ccatcgagcg
       61 tgctcgtccc ccgcccgatc tcgtcgagga tgaccaggct tcgcggcgtc gcgttgttta
      121 agatatttgc gagttcgagc atctccacca tgaacgtgga ctgtccggag gcgagatcgt
      181 caaacgctcc cacccgggtg aagacccggt ccgcgatccc gatggtggcg tgccgggccg
      241 ggacgaaact tcccatctgg gccatgatgc agcagagcgc caccgcccgc atgtaggtgg
      301 acttgcccga catgttcgcc ccggtgatga tcatgatctg gtcgccggcg gcatcgagat
      361 ccgtgtcgtt cgggacgaac ggtaccggga ggtgccgctc caccaccggg tggcgcccgt
      421 ccctgatgat ggtccggccg ctctcctcga tcaccgggcg ggagtagccg tagcgggcgg
      481 cgacctcggc gagggccgcg tagacatcga gcagccccac cgcccgggcg gtcgcctgga
      541 gaccggggac ttccgctgcg agcgtccgga cgagttcggt gtagatctcc gcctcgaggg
      601 ccgagaggcg ctcttcggcg gtcgcgatca tcgcctcctt ctcccggagg tcgggtatcg
      661 tgtagcgctc gccgttcgag gtcgtctgcc ggcgctcgta ctccggcggc accaggtgca
      721 ggttcggtct cgtcacctcg atgtagtacc caaagacccg gttgtaaccg accttgaggg
      781 acttaatccc ggtcctctcc cgctcctgct gctggaactc cacgatccag tctttcccgg
      841 acgtcgcgag gtgccggagt tcgtcgagtt tctcgttgaa cccttcgcgg atcatccccc
      901 ccgactttgc gaccgccggg ggctcgtcca cgatcgcccg cccgacaaga tccgtgaccc
      961 gggcgtggtc gctcatcgca tcgagggcct cgcggaccag ggccggggcg tcggcggcga
     1021 agagcgcctt tagatccggg atcgcttcaa gcgactcctt gagcgtcccg agatcccggg
     1081 ggccggcgtt cccgtaggcg atccttcctg caatccgctc gatatcggcg aagtcgccga
     1141 gcgtcgtgcg aagcgcctgc cgatcgagcg cgtgttcgag gagccactcc accgcatcga
     1201 gcctcccctc gatcgtcctc ttcccgagca gcgggctgat caggaacgac cgcatcgtcc
     1261 ggcttcccat cgaggtctcc gtgacgtcga gcgcggagag aagcgtgctt gcatcgtcaa
     1321 cgccccggat gctcgtcgtg atctccaggt tccggagggt gatggcgtcg agcatcatgt
     1381 tccccgacgg gatccgtgtt gcgatgccgg agatgtgggt gaggggcgac tgctgggtct
     1441 cctgggcgta gcggagcgcc gccccggccg cggcgaccgc tcccgtcatc ccggcgcacc
     1501 cgtagccgtc gagcgtcgcc gccccgaact gctcgcagag acgctcgcac gctgcatcgg
     1561 cgtcgaaggc gtcgtcgcgg tagcggctca ccgtcacccc gagggtttcg agccggttgg
     1621 ggagcgcgtc cgcgaggctt tcggggacga tcgcttccga cgggcggtac ctgaccacct
     1681 ccgagacgat ctcggcgtag tcgcgcccgc cgctccccgc ggagacgaag aactcgccgg
     1741 tggagacgtc caggaacgcg aggccgaagg tatctttccg gtcgggggcg accgccatca
     1801 ggtactgggc tcccgctgag ccgagcatcg aggagtcgat cagcgttccc ggggtgatca
     1861 ccctggtgac gtcccgcttc accacgcctt tcgcggtctt cgggtcctct atctggtcac
     1921 agatcaccac cttgtagccc ttgttcacca ggcgggcgat gtagccgtcg gccgcgtggt
     1981 gggggacgcc cgcgagcggc atccggtcgc cgttcttgtc cctgccccgg gccgtcaggg
     2041 tgatgtcgag ttcccgggcc accacgccgg cgtcctcacc gaaggtctcg aagaagtctc
     2101 ccatccggaa gaagaggact gcgtcggggt atcgggcctt cactgaatag tattgccgca
     2161 ttgccggggt cggtccgtcg gtcatcgcat ctccctatag aatccgcacg gagtcacata
     2221 agggtactgc cccgggaaat ccggcaaagt gggctccgat tcactgcacc acccgggcgg
     2281 gcctgccaag gtacatacgt ccgaagtgcg tacctggaac acgatactct atggcagcga
     2341 cgatcgcaga aaagatattt tcgcaacggt gcggcaggac tgtccgcgcg ggagacgtag
     2401 tgatggcacc ggtggatgcg gcgatgatcc acgacatcac cggcccgctt gcggtccggg
     2461 tctttcggga gatgggtgga gaacgagtct atgatcccgg gcggatcatc atgctcttcg
     2521 accaccagat ccccgccgac tccatacccg ctgccgagaa ccacgtcttc atgcggcagt
     2581 ttgcagagga gcaggggatc cacaactacg acctcctcga gggcgtctgc caccaggtcg
     2641 tgatggagaa agggcgggcg gcgcccggcg agatcatcgt cggcaccgac tcccacacct
     2701 gcacctacgg tgccgcgggc gcgtttgcga ccggcatcgg ctcgaccgat atggggttcg
     2761 tgctgaagtt cggggcgctc tacttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nngcgctcga gttcaccggc ggggccatgc gcgagatgga catggcgggc
     2881 cggatgacct gcgccaacat ggcgatcgag atgggggcga aggcggggat cgtgccgccc
     2941 gatgcgacca cctgggatta cgtcaccgcc cgccgcgaga tcgagccgtt cgacctcgcg
     3001 ggcgacgctg atgcggtcta cgcagagcgc cggcagtacg atgtcaccga tctcgcgccg
     3061 caggttgccg tcccccacaa cgtggacaac gtcgtggacg tgggcgacgt ggcggggacg
     3121 aaggtcgacc aggtattcat cggctcctgc accaacggcc ggtacgagga cctcgccgag
     3181 gcggcggagg tgctcgggac cgctgatgcc ttcgccaccg gtgtcagggt gatcgtcatc
     3241 ccggcctcgc ggaccgagta cttgaaggcc ctccgggccg ggctcatcga gcggttcgtc
     3301 gaggccggag cccttgtgga agcaccgtgc tgcggcccct gcatgggcgg ggcgttcggc
     3361 ctcctcgccc ccggcgaggt ctcgctctcc acgtcgaacc ggaacttccg gggcaggcag
     3421 gggagcacgc aggcgtcggt ctacctcgcc tcgccggcga cggcggcggc aagcgccctc
     3481 tacggtgaga tcaccgaccc gcggggggtg taatcatgcg gatatggaag tttggcgacg
     3541 atatcgatac ggacgcgatc atcccgggca ggttcctgac gatctacgat ccggcggagc
     3601 ttgcgaagca cgcgtttgaa gggacgagag acgagtttgc cagggaagtg cgggaaggcg
     3661 acgtcgtggc ggggggcagc aacttcggct gcggctcctc ccgcgagcac gcgccgctcg
     3721 cgctcctcgg ggccggtatc agggtcgtgg tcgcaaagtc ctttgcccgg atcttctacc
     3781 ggaacgccgt caataccggg ctcctgccgc tggtctgccc ggaggccgac gagatccggg
     3841 acggcgacgc cgtctcggtg gatatcgccg ggggctttat catggtgaac gggaaacggt
     3901 tcgccgtcga gccggtcccc ggattcctca aaacgatcgt cgaggccggc ggcctcgtgg
     3961 cctacgcaaa agatctggat gaggtggaga catgcagcac cgagtagcgg ccatcggcgg
     4021 ggacggcatc ggccccgaga ttgtggcggc gggaaaagag gtccttgacg ccgcaggaga
     4081 gcgctttggg ttcgatatcg cgtggaccga cttcgatatc ggggcggagc gctacctcgc
     4141 gaccggggac ctcctcaccg aggaggatat ccgggaactc tcgaagtttt cagcgatcta
     4201 cttcgggtcc atcggcgata cacgggtgaa gcccggggtc ctcgagaagg gcatcctgct
     4261 tgcgatccgg ttccacttcg accagtacat caacctccgg ccgatcaagc tcctcgacgg
     4321 cgtcgcgaca ccgctcgcga acaaaagacc ggaggatatc gacttcgtgg tggtccgcga
     4381 gaacaccgag gacttctacg tcgggatcgg ctcgcgattc tccggtaaac gcgagaaaaa
     4441 gaccctcgaa gtcgtccgcg acctctacag cgtcaagttc gggctggacg tcgagaccga
     4501 cgccgacgag ctcgcttacc agatcggcgt gatcacccgg ccgggtgcag agcgggtgat
     4561 ccgctacgcc ttcgacctcg ccgggagacg gcagaagaaa ctcacctcgg tcgacaaagc
     4621 aaacgtcctc tccgacgtct acggcctctg gcgcgacgtc ttcacggacg tcgccgccgg
     4681 gtacccggac atcgcgacgg agttcaactt cgtcgacgcc gtcacgatgt ggttcgtcaa
     4741 gaaccccgaa tggttcgacg tcgtcgtcac cccgaacatg ttcggcgaca tcatcaccga
     4801 cctcggcgcc atgatccagg gcgggctcgg gctcgccccc ggcgggaaca tcaacccgga
     4861 gggcacctcc atgttcgagc cgatccacgg ctccgcgccg aagtaccggg gcctcgacgt
     4921 tgcaaacccg ctcgccaccg tctgggcggg ctcgatgctc ctcgaccaca tcggcgagca
     4981 cgatgctgcc gcggcggtcc tcagggcgat cgagcaaagc atcctcgacg ggttcgtcac
     5041 ccgggatatg ggcggcgcaa agaagacgag cgaggtcgga caatacctcg cggcgcttgt
     5101 gaagacggtg tagggcggta gccctctctt tttttatgga aaaccggccc ggtgcgtccg
     5161 gtctactcct ctgaaccgga agtggtggag aagttgagtt gccactcccg gtagcctccc
     5221 tgccccgccg tcacttctcc ttcggcaggc atccagccct gcgggtcgcc ggtgatcgtg
     5281 agagagtaag catcggagta tcgattttgc ccggcaaaat cgaatccttc aagccaccgg
     5341 tttgacccct caatttccga cgagatcgag acctgcgtcc cgttctcgta gcgcgcgtag
     5401 acggggatgg tgtaggagta ccggtatcct tcggggttgt aataaccgcg ctcgttgggc
     5461 gttccggagg aacttgctgt cagtgtgagg ttctgcttcg gggcgaagag cgactcgttg
     5521 ccgaatgggt gccgtgtatc gatcaactgc gggacgacct cggggcgtaa tttgtcacca
     5581 agagaggtgc gattagagta gcgaacaccg atcggttctg cgggattcat gtagggagtg
     5641 gtcagccgga ggtagtactg gccgtcgacc gggacgatcg cgaaatcgta gtgctccgga
     5701 agagtcgagg gatcggaggg atgttttcgg aggttgccgg gatcggtgta aagttcacca
     5761 gaaatttgtt cgggaccgat gaccggctgg tcgccgcggg tcgggatggg tacaaggagg
     5821 gtcacgttct cgatgggcgt ggctgtccag atggagagtt cgtatctgaa atatgtataa
     5881 ggatcttgag gttcccccga gagacagccg cagaggaaga cctgggcaac gacgagggcg
     5941 aggaggacga cggggagcct gaagcgcatg caggaatctg ttcnntcatt tcctgccatc
     6001 ctctgccgca tcaggccgag gcatccgttc tactcccctg aaccggaagt ggcggaggcg
     6061 ttgagttgcc actcccggta gtttccgtgc ccagtccgta cttctcctcc ggcaggcatc
     6121 cagccctgcg gctcgccggt gatgacgaga cgataatggt cggtatatcg attcagcgcc
     6181 catgcatcaa actgttcagc ccatccgttc tcacccataa tatccgaaga gatctcgact
     6241 cggcttccgt tctcgtactg cgcgtagacg gggatggtgt aggagtatcg gcatccaggg
     6301 ttgataccgc gtgtttcaac cgcctccggg gagcctgcgg tcagggcgag gttctgcttg
     6361 ggggagaaga gcgactcgtt gccaagcggg tgcagcgtat tgatcatctg cgggacgata
     6421 tcaggccgga gatcacctgc aaggaaggta tagttttcat agttcaccca cacaggttct
     6481 gcagggctca ggaacggggc agttaaccgg aggtagtact gaccgtcgac aggaacgact
     6541 gcgagggtgc aatattccgg aatagtcgag ggataggagc gtacctccgg gagtttttcg
     6601 gaataaaacg catcggagat cgggctatgg ccgataaccg gccggtcacc gcgggtcggg
     6661 atgggaacaa ggagggtcac gttctcgata cgggtatccg ttgtgatgtg gagagtatat
     6721 gatgaggata cgctgaaatc ttgaggtccc cccgagatac agccgcacag gaagaccagg
     6781 gcaacgacga gggcgaggag gacgatgggg agccggcaac gcatgcagga atctgttctc
     6841 tccgggtata cattcatttc cccggaggcg ttccctggac ggttatcggt tcatttcctg
     6901 ccatcctctg ccgcatcagg ccgaggcatc cgttctactc ccctgaaccg gaagtggcgg
     6961 aggcgttgag ttgccactcc cggtagaccc cctgccccgc cgtcaccact ccttcggcag
     7021 gcatccagcc ctgcggctcg ccggtgatga cgagacgata ctgatcggta tattgattcg
     7081 gcgcccatgc gtcaaactgc tcgagccatt cgttttcgcc ccgaatatcc gaatagatct
     7141 cgattcggct tccgttctcg tagtgcgcgt agacagggat ggtataggag taactgtagc
     7201 cagggttgat accgcgtgtt tcaacgattc ccggggtgcc tgcgatcagg gtgaggttct
     7261 gcttcggaga gaagagcgac tcgttaccga acgggtaccg tgtttcgatc aactgcggga
     7321 caatctcggg gcggaaatca ccaagagagg tgcgattata atagcgcaca ccgatcacct
     7381 ctgtgggatc catggaggga gtggtcagcc ggaggtagta tagaccgtca acaggaacga
     7441 tcgcgcaatc atagcgttcg gggaggcgat cggcataaaa tgcatcggag atcgggtcat
     7501 gacctagggc gggccggtcg ccgcgggtcg ggatagggac gaggagggtc acgttctcaa
     7561 tgggcgtggt tgtcttaatg gagaattcgt actcaaaatg tgtgtgaaga aagtcaacct
     7621 ccttcatgga acagccgcag aggaagacca gggcaacgac gagggcgagg aggacaacgg
     7681 ggagccggca acgcatgcag gaatctgttc cttcctggta tacattcatt tccccggaga
     7741 tgctccttga acggtcatcg gttcactttg ggccagatcc cgaacatagg gcagatcgtt
     7801 caggccgttg agcgtgatcg agctctccct gcattcgatg atcttttcgg gtctcttcga
     7861 agcgagggat attcaccaca tccgcgaaca ttcatcatag caataaataa gccatttaga
     7921 ac
//
LOCUS       DAJB01000214            9121 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_45475, whole genome shotgun sequence.
ACCESSION   DAJB01000214 DAJB01000000
VERSION     DAJB01000214.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_45475"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2995..3082
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1529 a         2910 c         2891 g         1699 t
ORIGIN      
        1 ggaaagacgg gatataacta ttccgctatc gtccacgcat ctcctccgtg cggccgggtc
       61 gttcgccctc tcctctgctg ctcgtcccgg ttacggcaac cggccctgcc ccgaggtcaa
      121 ggaggaaccc cactttggga gcgtgttcat catgtggtcg gaagttacat cggagaagga
      181 gcgcccgaat cggtcctctc ggccggcggc gagatccggg acttcacgcc ccggttccgg
      241 gagtcggcct acaccccggt caaaaaggtc gtggacgagg acaaaaggga ttttgaagag
      301 ataatgatcg gggtcgagcg ggcggcggag gcggcgcttg caacgaagct ccgggcctcg
      361 taaagaaaca gatccctgtc aaagtgcggg atcggtgagt ataagtgtga gatttgggag
      421 agggttatcg caagagatcg taaatttgtt cttttgtcat accctcgaat tctgcaacat
      481 cggtgacgat tgtgttgagc gtagccgtac tgagttcgtc gtgaaggggc acagtaaccc
      541 ggagtcgttt atctccaaca actttacgca tctggacgtg acttccgacc tgccggagaa
      601 aagaaaatcc gagtttttca aggactctga tggtctcaaa cccactaaca accgggtgcc
      661 gcggcaatga tatcgacctt gcattcgaaa tttgatggga tctggctctc ctgttcgcgg
      721 gtttcggatt tgtatctggt tacaatggtg atctgttctc ccgcttcgag ttcttccgtg
      781 taatggagcg acgttgcctc caggatattg tcgatgagtt ctccgatgct tttaccctgg
      841 gtatagatgg cattttcagg tgcatgtgcg ctggcactcc accatccttc agcataggtt
      901 acttcaaatg tgaccagcat gactctgcac ctctgtgtga ttgggatatt gggtttttca
      961 gagataagtt tgatggtaac gtggtcatca catccagagt aggttatgtt ttaggtccgc
     1021 gtcggggacg gagggacggc ccgtgcgtcc ccctcacaga cgtgacctaa ctctccggtt
     1081 ttttcccggc cgcacagacg ttgtaggcga ggtagggcag gttctcctcg acgaacggct
     1141 ccaccttccg ggcgagttta aagaccgggt ctgcaaggtt taagtgcgca aacgagcacc
     1201 ggccgaccgc gacatcctca aacccggctt cctcgaagag ccccgcgatc tcggcgttgg
     1261 tgtagtaatg ctcgtcgaag tacccgacac ccaccttctt taagccgacc ttctccccca
     1321 cctggtagac gatggggacg atgctcgtaa agaccgtccg tgagagggtg cagatggcga
     1381 tccggccgcc gggcttcaag acccggtagc actcgcggag catcgccgca gcgtcgggga
     1441 cgtaactgaa ggcgagcagg cttgcaaggg cgtcgaaggt ggcgtcgtgg aacgggagga
     1501 cgtcggcggt cccgacgcag aacccgctct ccgggcagcg ctgccacccg tgcctgacca
     1561 tccccgggct gatatcgagg cccaccgcac ggccgccctc ctcgacgtag cgctgcacga
     1621 agagcccggt cccgcacccg aggtcgagga gaaactttcc cttcgggaga gcgctcatca
     1681 cgtggtcgga gatgtgccca tggtagtact tcccgcggtt gccgtcgtag cggtcgtcgt
     1741 agatatccgc gacctcgtca tagtgttgct gtactttctt caccgaacac ctcccgggca
     1801 atccgggcaa gaagtgcatt gatctgcaca aagtcgtttg cattctggca gagtttgagg
     1861 tccgcatcgg cgagcgcgat ggcaagggcc gggtggttgt actcgcgccg gaccacctgc
     1921 cggagttcgc ggacgacctc ccgcgccgaa agcccgtact cgatcatcag cgactcgaca
     1981 atccggcgtg cggcatcgaa gttgccggcc cggagcgtgt cgaacgcaga cgtggcaacg
     2041 ttcgcggtct ccgagcgcga gacctcagcg aggtcgagat cctttcccga gccgacggtg
     2101 atctgcaggt acatgatcgc ccgccgcaga tccccctgtg cggcgtggac gatcaggtcg
     2161 aggtcgtcga caggacatgc ggccccttcc gcagcgagga tctcttcgag ccgggcgcgg
     2221 acgatcccgc tctcaagcgg ggcgaagaag agcggcaggc agcgcgacgc gatggcaggg
     2281 atgatcgccg acggccggac cgtgacgagg atgaaccggc aggtggcgct gtagcgctcc
     2341 atcgtccggc gcagggcctg ctgcgcctcg aacgtcaggc cctgggcgtc ctcaaagacc
     2401 atcagtttga agtcggcgtc aagcgggcgc atcgaagcgt accacttcac gatccgcttg
     2461 aagttgacga tcaggctccg gtctttgcgg tagatcatgc tgaaccgctc gtccgtctcg
     2521 agagcgctct tccctctccc gaggatgtcg gcggcgctga ggatggtcgt gttcgcctcc
     2581 cagttctcgc cgtaaagcct tctggccagg cactcgacgg ccacggtctt cccggtgccg
     2641 tgcgggccgg agatgagcat atgcggcacg ctgccggagt ccgcaaacgc cgtgaggcag
     2701 cggacgacct catcctgccc gatgatttca tcgcaccgct tcgggcggta gacctcactc
     2761 cagagcatgg gccagtctct ctctcatctc atctattgcc gcccggagaa gataggtatt
     2821 gtcaagcgag gcgacaagcc gttcaccctc gccgggacca agagaggagc acgccgcggt
     2881 tatcgcgggg atggcacgcg tcacctcgtc gacgaccgtc agggtcgccg tgcgggcggt
     2941 ccgagagagc gggtttagat cgatcgtgat gacggtcttc cccatcgccc ggagnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nncgctcctc tgtccggtga aagaggttca cctcgacgtc
     3121 ggcgccgctt gcccgctgga gcgccgcgac ctcatctgcc gcaagcgccg ccgtgttccc
     3181 gttcaccgat atcaccgcgt gccgggcaga taaaagcatc gcggcggccg tccgggcggc
     3241 gagagcagcg ctcttcgtcg tcctctcgcc gatcaggtag tcgaacgcct caccgcgccc
     3301 gtgggcggcg agcccttcca tggcgacgat gccctcgcgg gcgcaccggg ccagccgctc
     3361 gcgggcgacc agggagcgat agcgtggatg gtctttggag atcatggttt catctcgatt
     3421 atgtatgcgc cgcggcgtgc gacgcggaga cggtagacct ccccgaaccc ggagagcacc
     3481 cgctccgcag cctcaccgca ggcaaagaca ccgtttccaa gcatcgtcat gcttgccgga
     3541 accccggcgg cgtcgcaggc ctcgagcacc cggtgcacct ccggcgcgat gagcccgctc
     3601 ttctcggcga accctcgcga gaggaggcag aagtcgatga ggtcccgtgg gcagcggtcg
     3661 ggatacgcgg cggctatccg cgccatcgcc ccctccgacg agaggaccga ggcggtcggg
     3721 agcgggccga ggctcacggc atatagaggt tcctccgggt agaaccggat gatgttcgcg
     3781 tgaatgcccg gccccgtcct gcagacgacc ccgcctgnna ggtgatttgc cgccgtgagc
     3841 gttgatagaa gcgccgctgc cgagaggccg aacccggcgc cgatggggag ccggcactcc
     3901 gtggtgacgc tggcggcgac gcccagctgt tcgagggcgt actcgaccgg cggcgacccg
     3961 gtgcttgcat ggcccggccg gacaacccgg accgtcgtct ccgcggcctt cgcgaccgtc
     4021 gagcgcaccc cttcgtcgat gaccacgccg gccccgacgc tcccggtgct cgaggggtcg
     4081 ctcccttcga tccgcctgaa atagccggat atgtgcccgg gggagaaggc tacagcagtc
     4141 cggaccatac cgctgctgcg acctcctctt tgctccccgt cacgtcggtt gccccgccgg
     4201 cggtcatgat gcaaaacgac ccctcggccg cccccatcgc cggtggagcg ttcacgacga
     4261 ccatccgggc acccgcatcg agcattgctc tcgcccgctc ttcctcgtcc caccccagtt
     4321 tgaacgccac cgtcacggga cggcacgccg ccatcacctc gtcgatgacc ttcgggaggg
     4381 ggtcgagcct gaccgtcagg ggcgagccgc tcgggatctt cccctcccgc cgcccggggg
     4441 caaagtccga tatcgctgcc gcgctgacgt agatgtcgat cccttcgcgg cagacagcga
     4501 ggacagcctc acgcatctct ccggcggtcg tgatatggat gtttcggacg cacgggaacg
     4561 agccgctgtg caccaccgtc acctcggcac cgagccggaa ggcctcgagc gcgagcgcct
     4621 gacccatccg tccggtcgac cgggtggtca gcacccgcac gtcgtcgagg ggttccgcac
     4681 acgccccgct cgtgatcagg acttttttgc ccgcgagcgg ccggccggag accgcccggg
     4741 cggcgtggag gacgatgacg tcgttgtcgg cgatcttcgc cttcccctcc tcgaccttcg
     4801 ggtcgacgac gtcgatgccc caggagcgga gccgccggag gttctccttg atcccggggt
     4861 ggcggtacat gctctcgtgc atcgcgggag cgaccannat cttcccgatg gtgtttgccg
     4921 tgcagggggc gaccagcagg aggtccgccg ccccgccctc accgcagtag aggacatgct
     4981 caacgaggcc ggtgatccgg gttattgccg gccgtccggt cgcgtaggtc agggcgtcgg
     5041 ggtggacgat ccccgctgcc gcctccgtca tgaccgcctg caccgttgcg ccccggcgcc
     5101 gcagggcgtg agcgagtttc accgtctcga cggctgcgat gctccccgtc acggcaagga
     5161 ccaccgtctt tcccgaaagt gtcttcacga taccgtcaca tcctgcacca tatgccacgc
     5221 gtgcgggccg tacttcttca cccggcggga ggttatcgcc gccgcaaacc cggcttcccc
     5281 ggcctgttcc cgtatcgcgg ccgcagtatc gccgatactg tggacatgga gcacgctccc
     5341 gctccggaca tgggcgaggg cgtcggcgag catcgacggg gcttcgaaat gccccatcag
     5401 gacccggtcg tagaccccgg caagaagcgt ccgcgagtcc ccgacctctg ccgtgacacg
     5461 gtccgcgaca tggtttgcca taatgtttcg tttcaggtat tcaaaggctg tcggattgat
     5521 ctccatcgca tggacccgtg cgccgcttac ggctgcgggt atggagaagt agccgatccc
     5581 ggcgaacata tcggcgaccc tttccccggg ccggacgaga gcggcaatcc tcgccttctc
     5641 cgtgcggttt ccctgcgcaa acatcacccg tgtcgggtcg aggatgaagg tgtagccctg
     5701 ctcgcggtgc cgcacctcgc cggcggtgcc gtagagcact tctgcgtccg ggatgcgcat
     5761 tgcgccgaga aaatccttca cccggacgac tccccggggg tggcagtgcc ggatgatccc
     5821 ggcgagttcc tcctcagacg ggagagggcc gtggagcacc gcgatatcgc cgacgaggtg
     5881 gtagccgcgc ccccggtagg tctcccgctc ggggaggttc gcgtcggccg gatacccctc
     5941 cctgaccggc acccaggcgg tgccgccctc gacatacggc cggcgggtgc ggtccaccca
     6001 ctctgcatcg agacaatcgg tgagggcgcc cgccggcacc ttcctcgcac gcatggcgac
     6061 actcacccga cgatgatcag gcggtcctgc tcctcgtcgc ggagcacccg gatcggctgc
     6121 ccctccaccg ggaacgtcca gggaacgggg gtcagttccc gcgtcttgta ggtctttgga
     6181 tccatgagcc ccaccaggcc cggctgcgtg aagacgacga gtgccgcctc ggcatcacgg
     6241 acgctgccga tgatgcgctc gatctcgctc tcggcaaggg tccgggtcat gccggtctgg
     6301 aggtcgaaga cccggaggcg ctgcccgtcg acatcgcgca cctcgaagta gttgccccgg
     6361 gataccacca cgtccccttt ctgatagtag gggaggcgga tggagtaggt gatgcggtag
     6421 agcgccttgc cctccttctc acccatcagc ttcgggtggg tcgtataccg cccgccgagc
     6481 gcccccgtta tcgcccgggt gagcatctgg ccgaggtgct gggacccgac ggtgatatcg
     6541 acgccgtccc tcgactcctt gagttccgat atgaacgaga gacggtcccc cgactcctgg
     6601 agagagtctt cggtctgctc tgcgatgctc gtggcgacct cgcgctcgta ggcgttgatc
     6661 tttctgttcg tagcccgcac ctggacgatc ccctcgtagt agccgccgga gatgcggctg
     6721 caccggtcgc aggcctcctt ctgccagtgg atctcgacct ggcaggtctc gtggaccggg
     6781 atggaataga gggtcccttc cacgtcgacg gtgcaggccg tcctgttcga cgcgatctcc
     6841 tcggatcgga cggtgaccct ggggttcttc acatcctcgt ggatgctgac cgccgatatc
     6901 gccatttcgg tgatgagatc ttctcgcggc gtccgcaggt ccgaccaggt cttgccgcgc
     6961 ttctgcgagt cgcagacagg gcagtagacg gcggtcacgt agttctcgca ggtaagcacc
     7021 cgggtatccg cgacccggca ccgggggcag agcccttcct cggttggttg cccacaacgg
     7081 gggcagatga tcgtctgtat ggtcatggat cagatcctga ctatctgggc atggggtaca
     7141 tcgccgatca tgatgcagtc ttcgttggtg atcagcccca tagcgatggc gagggctacg
     7201 acgcgcttcc cgataatgtt aacgttcgtc gcgtcagcga gagcctctct gacttcttcc
     7261 tcggcggcac actcggtgcc gtaaaatcct ccggtgatgc agacctctac gtctccgtgc
     7321 atcagggtga cgtttaagag ctcggagtcg caggcagcca ccacctcgcc ggcgcccggc
     7381 atgcggtgaa ctttcaagta cattgcagat ctcttgggcg tgagtcgtca aaaaggtgcg
     7441 ctgttgtcta gggaaggacg atatccacgc cgtaggtgcg cgacggggtc ccggcatgga
     7501 tgcgccaggc atcctcttcg gtgataagcc cctcatcgag gtaccggcgc gtgaaccgcg
     7561 ggaccgattt cggcccgatc accgcgccgg gcctcgcgtt ctcgtccatg taatcgctct
     7621 ccatggtgaa ccatcgcccg gcccgggcga gcgcggggat atcctcgtgc cgcgcgatga
     7681 aggagggcat gagcggcgta tcgggggttg cgaagtgttt gacaacctgc tcgatcggga
     7741 tccccgcccg gccggccatc tcgacgacgt cggtgcaggg cccgctctcg gcatggagct
     7801 gcacggcgca gccgcactca cccccgagtt cgagcgcgtg gaagaggacc gtgttcgatg
     7861 ccgcgagcac ctcgggcgcg acctcgtagt ggggccgccc gcttttgagg gcgacagccc
     7921 ttccttcctc gacgtaacgg gcggcaaggt cgagcccggc catcatgatg gcggcggcct
     7981 cgtcaaggct catccgtccg gcgaggcggc tcatctctgc cgggtggatg ccgaggaccg
     8041 ggtagacgac gagcccggtc tcccggatca tatcggccac ccggagcgtc gcctcgaaga
     8101 cctcccggta gtcttcgccc gacgcggcct cgaccccgaa cgaccaggaa ggcttcgtga
     8161 ccaggaagat atgcgtcccc ccgctgcggc ggaagtcctt cgcggcctcg acgccccggc
     8221 cgttttgcgg gtcgatgtgg atatggtcgt cagtgatcgg tatcgcgggg ggtttcatgg
     8281 aactcaacaa tctccatcat ggggtagtcg tcctcgaggt gcagccgggc ccggcagacg
     8341 gtctcgccga cctccgtcgt gatagcgacg tcgaacatcc gcccggcaag ctccgagtag
     8401 ccgaaccggt tcgagaccat caggtcgcgg ttcagctgga cagagatctc cgtcacgtag
     8461 ggctgcaggc cgacggcctg ttcgatgacg gtctcaacgg tcgcggcggt ctcgcgggcg
     8521 atgggcgtcc cgacgaactg gtggtagagg gcgccgagtt tgatcccggc ttcaaatgcc
     8581 gccttctcgc ggtcggtgat catggtagtt aggtttggtg tgcagggtaa taaggggctg
     8641 tcgccggggg tggatatgac tgcttacagg aggaggccaa aaccgggtgg tcccgctgaa
     8701 aaacgacctg ccgcaggttg cgacgttcca acggaacgga gcttgaaaag tcgtcagtag
     8761 tgccctacga aacaactgat aatactttgt taagatccca tccgggtgct cccgattgat
     8821 tgcaatccgg agcacgtctt tccatcgggc tgcgcacctg gcggtgctcg agctccgttc
     8881 ctgtcggaac gtcacgtatc gccatgactg cccccgcccc cgggggcggg ggaggaggcc
     8941 ggaggccggg cggagtgggg gttacgggta tcccattcgg cagtagagac aggggagggg
     9001 gagaccccct ccccgtcagc cccacccccg cgaggcgata tccccacggt ccacttgcag
     9061 gggcatgccc ggggtgattc ctcgtcccgg gtcgatgctt ccatattcat gccctattca
     9121 a
//
LOCUS       DAJB01000215            5455 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_108453, whole genome shotgun sequence.
ACCESSION   DAJB01000215 DAJB01000000
VERSION     DAJB01000215.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_108453"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          912 a         1795 c         1738 g         1010 t
ORIGIN      
        1 tacttcgacg ccgacgccga ccggttcgtc gagacgacgg cgatctccgg ggggagcggc
       61 gtcctccccg ccaacccggg gctgaagccc gcggcgctct ttgcggctgt cgccctgatg
      121 acggtctcgc tcctgatcgg ctacgccttc ctgaccatct acgcctaccc ggcagtcttc
      181 ctcgccttcg gcatcgccgg caacctgctg ggatggttct acacggcgcc gccgatcgcg
      241 cttgcgtacc ataaactcgg ggaggtcacc aacatgatca ccttcggcct cctgatgccg
      301 ggcgcgggct acttcgtcgc gacggggacg ctcgacctcg ccttcttcgc atttgccctc
      361 ccgctctgtc tctacgggct tgtcttcatc gtgagcgtcg agatcccgga tatggaaggt
      421 gacttcgccg gggggaagcg gaccctcgtc gtgcggaagg gccggatcta cggattttac
      481 ctcatcaccc tcgcggcgac ccttgcgacc gcgacgctcc tcctctttgc gctcaccggg
      541 acttttgccc ctgtggactt ccggccggtg gcgctgatct ccgtgatccc gctcgggctc
      601 gctctgtggg ggttctcgaa ccggtgtgcc ggacgggcat gcgccctccg gtacgccacg
      661 gcgaatatca tgggctattt cgcatttcag gggctggtag tcctctactt cgtcctcgcg
      721 gccactcagg cctgaccggg ctgcacctcc accgtccgct cgaaggggac ctcgaaggtc
      781 gcaatgccga agtcaaggac tcccgacccc gtcacccgga gcgtgaccgc tccgtccgcg
      841 aggccccgga ggagtccctg cacgagccgc aggttatcga cggtcaccgg gatggagatt
      901 gccgtctcgg catcgggctg gattgcaaac cctccccgct ctccgtgcgc gaggaagacc
      961 cattctccgc cgtcgaggaa gtagacgtcg aacgagaccc gggtcagggt ggcgccgatg
     1021 gggttggggt tgtggatgat gaggtgcagc gagaggtcgg tgcttcgtgg ggtgacattc
     1081 tctatctcga ccccgctgag ggtgatggtc ggctcctgga gcgcgggaac cgcacaaccg
     1141 gcgctgcata gcaggacggc gacaaggagg gtgacggcgg ggcgccagat gcaccctgcc
     1201 gggatagagg tctgccgtgg cctcatggtc tccctcctat gagccgcccc cgtaggagag
     1261 caccggttcg atgaggacgt acttcctgac gatgatgttg tcggcgtaga agtggtgcgg
     1321 gtcgccggag taccacgagg aagacccgat cagcgcgaag tcgtcaaaga ttgtctcgct
     1381 cgagaccagc gagccgtcgg cgaatcgcat gggcgcactc cccaggtact cgccgtccat
     1441 ccatgcgtgt tcggtggcac gcggcgtatc cagaacgacc ttgatgtgat accaggtatc
     1501 ggtcctggcc cgggcgtcgg cggagaagtt ccggtgttcc ccgtcgtagt gctgcacgga
     1561 accgttccgg aggtgaaggg catacaggtg gttggtcccg ttccaggcct ggatgtagcc
     1621 cgaaccgcac caccgcccga actcactgac ccggaagtcc ccttcgatga ccagcggacc
     1681 tgccgagatg ttcgtgagac atacccggtt gctgccgaac tccgggaggt cgggctcgga
     1741 gacgctgaga tccccggcgt atgcaccgcc gcggacgacg tccgactgga tcctgccgtt
     1801 cgaaccgctg aacgaccacc gtgagagatc gcccacctca tagtcctcaa agaagaggaa
     1861 gacggcgtcg ggatcgcttg cgctcccggc ctccgggttg ccgtagagca tggtgatcgc
     1921 aggcgtcttc gccggaaggg cgagccagac acgggcgtag gcaccttcgc tgacattctc
     1981 tgcccagtag gggatgggga tcgcttgagt gtccacgaac cggatatcgg tgaagtccgg
     2041 ccgcatcccc gggaggtggg tggcgttgaa ggagaccgca agaccatcgg tgccggaggg
     2101 gtcgccaacc gagattgcct gctgtcgggc gaaggtctcg acggtcgccg tggtggtgac
     2161 gacggatgtc ctggtcgcga tcgagagcgt cacgttgtag gagcccgggt ccgggtaggt
     2221 atgcaccacc gatcgttccc ccgtaccgga ggtctcctgc ctcgtcccgt cgccgaagga
     2281 ccagctccgg gagacaacgt cggcgatgcc ggacgtatcc gtaaactgga cactggcggg
     2341 ggtcccggcg tacgtgacgt ttgcggtaag cgccggtccg gtcgtgggca cggcgccggc
     2401 agggacgatg aggagcagca cgatgacccc tccgatgaga atgatccagc cgttatccat
     2461 gatgctgccc ccatgtgtcc ccgcccctat atgcgtgtag cggccggccg caccccaatg
     2521 ctgatgctcc gcggggaagc acggtacacc ggtgagtacc gtgaacgacg ccatcacgat
     2581 cgcgatcatc gggggaggcc ccgccggact cttctgcgct ctgcaggcag ccggggaagg
     2641 gaggagcgtg gtcctcttcg agaagaagcc cgccccgggg cgaaaactcc tggttgcggg
     2701 atcggggcag tgcaacatca cccatgacgg ggagatcgcc ggcttcctct ctcattacgg
     2761 tgatcacggg gcgtttctcc ggcccgcgct catgaacttc acgaaccggg accttatcgc
     2821 cttctttgcc gccagggggc tcacgatgaa gaccgaaccc ggggggaaag tctttccgga
     2881 gacccggagg tccgccgatg tcctggccgt cctcctcgcg gagtgcaccg cccgtggggt
     2941 ggacatccgg tgcgatgaag ccgtccagtc cgtcgaacgg gacgaagacg ggctcctcgt
     3001 ccggacacag aaggcgaccg tccgggccgg cgtgctcgtc ttagccaccg gcggggcctc
     3061 ctaccccgcc accggatcga ccggggacgg ctacgtcttc gcccgggccc tcgggcagcc
     3121 ggtcaaggag atggctccgg cgcttacccc ggtctacgtc gcggactacc cgttcgccga
     3181 ccttgccggg atatcgtttg agaacccgac tctcgctgtc taccgggacg ggaagctggt
     3241 tgcccggcat gccggcgacc tcctgctgac gcacaccggc ctctcgggcc cgggcatact
     3301 cgacgcctcg cgctacatcc tcccgggcga cgagctccgg gtcacgttcc tccccgggat
     3361 gaaggcggag gaggtgagga agcgggtcgc cgacggggtt gcggcgggtg gggtccggca
     3421 ggtaaagacc gtcctctgcg atctgcccct ccctgaacgg ttcgtccggc ggctgctcga
     3481 ccttgccggc gtcctgcagg gggagacctg tgcccagctc ccgaagaagg cccggaacgc
     3541 agtcgccgca tcggtcgcgg agttctcatt cagggtggag aggctcggcg gattttccga
     3601 ggcgatggtg acgaggggcg gggtcgccct cgagggggtc aacccaaaga cgatggcatc
     3661 aaagaccgtg caaaacctct actgcatcgg cgaggcgctc gatatcgacg gcgataccgg
     3721 aggctacaac ctccaggccg cgttctccac cgcggccctc gcagcgcggc atatcgccgg
     3781 tcttcaagca ccccggtgac cccaccggtc ctgggaatct tttttgcgcc ggctcaaagt
     3841 cccggcccgc cctcccgatc aacccggagc tggtccctgt taaggtgcgc tccggagtgc
     3901 cttttctcag cagtatctgg gcaaatctta tatccatcat atacatgggc gtatcaaaat
     3961 agaagtccta atataccgat gtaggcatta aaatatattg agacaataac gtgctttgga
     4021 gcattattaa cgcgattctt ctcctctcag tcctcatccc ccagggacac ctgatctacg
     4081 atgccgggtt tgccgatgcc gggataagcg ccaccgaagg gacttcagtg gacgacctgg
     4141 ccctcttttc tgcacctccc gaggtcctcc gggaatcttc ccccgatgca ctcatcgatg
     4201 cggtgggccg gacgacgttt gcctgcgtcg acggttcccc ccgcctcttc ccggtgggcc
     4261 tcaacgcgac ccttgcccgt ggggtaggcg actgcaccga cctcgcgctc ctcagggcgg
     4321 agatcctttg ccggaacggg atcgctgccc ggccggtcca cggcctcatg gtctgggatg
     4381 cggagacgtt ccggaccaat gccctccacg tggaggtctt tgggaggggc gttgccgtcc
     4441 acgactggat cgaactcggc gacggccgga cgatgggcgc ctacgaaggg agccccggga
     4501 tccggtgcgt aaagaccggg aacggggtct gtttccagcc ggtcttcgag gcattgggat
     4561 acctctgaac gggggttgaa cccggccttt gggaagattc aatatcagcc gcgccaaacg
     4621 gggggtatga gcttactcac agggctcctc gctctgatcc tggtggtcgt catcgcgttc
     4681 gtcctctaca aaatcgtcaa gagcgtgacg ggcctcatca tcaacgccgt cgtgggcgtg
     4741 atcctgctct ggttcatcaa cctcctcgac ctgatgagcc tcgtcggccg gcccgacata
     4801 ccgatcaacc ttatcaccgt cctgatctgc gctatcggcg ggatcttcgg cgttttgatc
     4861 acggtggtgc tccacctcct cgggataccg ctgacactct gaggagcgcc taagcggccg
     4921 gagcagcaat ctccacgatt gtccgggcga actcctcgat cgccgggccg aactgtccgg
     4981 catcgacctc gctctccagc gtggaggcag acgagacgaa ccgcatcccc tttgcttcga
     5041 ggcctttcct cacgaccgcg acggccttct ccgcgaggac cgagaacggt tttcccgagg
     5101 cgttcgagac cagagcaagg tactcgttga tgtagggcgg gaccttccgc gaccagaccg
     5161 gggttgcgac gaccaggtag tcgacaccgg cgagatccgt ctgcgccggg cgaatggctg
     5221 cccgcatccc gaacgccgcc atcacacccc ccctgaccac gcccggcccc ggggccggtt
     5281 cgatcctctc aagcctgccg ccgacctgct ccgcgcgcgc cgtcgccacc ttccccgtat
     5341 gtccctgcca tgagtagtat acgatgagga tcatcaccat cactccggat gggaggtgaa
     5401 ctacccccag cttacgcagg gggcttcctg cttcatcccc ctccccaccg ggagc
//
LOCUS       DAJB01000216            7801 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_106132, whole genome shotgun sequence.
ACCESSION   DAJB01000216 DAJB01000000
VERSION     DAJB01000216.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_106132"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1522 a         2439 c         2360 g         1480 t
ORIGIN      
        1 actccttgct acatctcttt gaacggctcc ggaaggttct tcaccgcctc tccgaggatc
       61 tccaggttcc tcgtgaccgc atcctgtgtc ctggtatcag cgagaaactc accataggtc
      121 atatcggagg tataccggag gatgcgctcg acggcctcgg cgatgtcgcc acagagaagg
      181 gggatatccg accgttcagg catacgcgac ctcggcggtt acctgctcac gggtaagcgg
      241 tttaagggca tcaggcgtca ccaggtcgac gggagcgccg agacggagcg agagatactc
      301 ttcgagttcg acgaaggtga agaacccgac cggccgggag aactcgacga ggatgtcgat
      361 atcgcttgcc tcggtctgct cgccgcgtgc gaccgagccg aagataccga tccggctgac
      421 tgagaaccgc gtggagagtt cgtctttgag ccgttgcaga gttgcgaaga cttcttcccg
      481 ggaccgcatg gtaactggta ttctgtcggg gaggtattaa tggattgctg caagagggga
      541 tgtggccgcc ggggcctccc gacgtctgat cccataaaat ggatgtcatc ctttgttgtt
      601 cgagtaggtg atcctgacct ccgcggtgtc gcgcaggaag ttgatgccca gcacctcggc
      661 gccgtagacg ttcagtccgg atctccgagt tcaggacggt atacagcgtt tcgacgtcgc
      721 tccagtttgc gtcaccttca ttggtcttct tctcgaaact tcccgtgtcg aacgtgccct
      781 gcgcaaacgt tgccccggtg ccttcgggtt tgtagacgtt gccggaatcg tcctcgaact
      841 gggtctctat caccgtatcc tcgacgctct cgaccatcgt gtagagcccg aagtagaccg
      901 gcccctcccc gtagtccacg tagatgcggt agaacgcagt atacggcgca gggactccgg
      961 actccatgaa tatcgacgga acgatcatat cccgcatcag tgagtcgtca gtgtacccgc
     1021 tctgcaggtt gagctcgtca aagccgtaga accgctggtt cttgattgcg gggttctcgt
     1081 cctcgaactt gccgaagttg agcttgaacg agatcttgcg ggatccttcc ttccacgtgc
     1141 tcccaagcga catctggcct ttgaaccgga tgccgacgtc cgtccatacc ttatcctcga
     1201 acgagacgtc cgcggggacg tagacgggat cgatatcgcc ggagatacct cccatgtcct
     1261 ggtctcctcc gggctgcctc cctccctgca cgtcgccccg ggcccccggt tcggcaggca
     1321 taccccctgt ggtgctcccg ccgaattcgc cgtagagttc ggtcatgttg tcgagcatct
     1381 tctgccaatt ctccggcgtg atcgtttgac ttcgtcgtca gaaaacacca cggagtaatc
     1441 cgggtcggca ctgctactgt gcgaggggtc cgcctggata tcgcctgtgt ccgggaccgt
     1501 cgtactgtcg tccccggcac ctgtcacagc ggtgcagccg ctgcaggcgg tgagaacagc
     1561 tacgatcaga gtcagggata cgagtcttgc cggcccccgt ctcatcatct cgggtacgaa
     1621 ctctgctatg ggtcgcacgt ggatacatag tttaacatta tgttaacaat actttactaa
     1681 atgtagttat ggatttccaa acgacaggcg atcgtactcc acctattctg ttgaaggacc
     1741 agggatcggg caggaaccgg catatcctcc tctcctggaa ccgcccccgg acatcggcct
     1801 ctccggcctc ccgcccgcgc cccggtatat gcccggccgg aagaacccaa cggggacgcc
     1861 gatggtcttc acggtgtgcg accacaccgg gtgactgaga atcccgcagc gatcccaccc
     1921 ttgaggcgct tgcggggcca cgaagacgcc tccagacgtt gcgtgaatgc caatggcgga
     1981 gactggtccc gttttcattc tgaaattcgg tgccgttcac gttttcaggt tttacgtccc
     2041 gttcccggag aaaaaagagc gagcgggcag cgggctctca agcgtccgtc atatgatctc
     2101 gatgacggcc tcgtccccgc tccctgcccc ttacgcacgc gccggattcg cgacccgtcc
     2161 catgaagagg accgtgccgg aatcattctc caaaatgagg aagatgaacg ggtggtccgc
     2221 ccggaatacg ggcgtatcgt tcggacgtac gctcgtcacg cccatgacga cgccggtcgc
     2281 cgccgcggcc tcggtgccct cctcgttgac gtcgacaaac gccttatgga cgacctcgct
     2341 gatgaagagg tcctgcgttc catccatccc cgagaagtcg gcagcatcag taaacgccgt
     2401 cggcatcccc attgccccga gcgttcccgg gaggctgtat gccgtctcaa gcgtgaactt
     2461 cgggaagaag accctgaccc gttcgcgaac cagcgactct cgcaggtccg cgaccgtctc
     2521 cgcatccagg ctctcctccg cagccgtcag attatcttcc ttcggaagaa ggacgagcat
     2581 cgagagttcc gttccgttcc cgtgcgcata cggtagctcg agcacctgga gggtgtcggt
     2641 ctccgtatac ccgtagacag catcctcgtc cgtccgctgc atcatcggga cgagcacggt
     2701 ttcgtttggt ccgacccgga actcctcttc gcttgtttcg ttcgcgtcga actcgttcgc
     2761 ccaggtgcct ttgaagtaga tcgcgttcgt gatcacgagg cgggtcaggg agtcgatcga
     2821 cccggcgggg aggaggtcgc ggatcctatc ctcggtcctc tcctccaccc agcggttgat
     2881 ggtcgcacgc gaagcctcgg ggctgctctt gaagtcaagg ttcgtgacgt tcgccccgta
     2941 ccaccgggcg gccacatcga cgtaatccgg gaggaacggg taggtctcct ccgcccagag
     3001 agcgtttgcg gtgcggagag tatagttttc gtccccgctg ttcaggatgg catcgagccc
     3061 ggcaaatccg gctctccgga gggtctcgtt ctccgggagg tggagcaccg atccgatctc
     3121 atcggcggtc gtgccgcggg cgccctcgca ggtgatcgcg agggccgacg agatgctgta
     3181 gggtgagaag aagaggttcc gaccctcaga atccggagcg ttcgcgagct gccggtagag
     3241 gtcgaccgca aaccgggtgt tccccgccgc cacgctgctg ttgggtgcca tatggttatc
     3301 cggtgtctcc tgcccgccag gaggggcgga ctctgcagac gtcgtttccg gcagatcggt
     3361 gcacccggcg gcgatacagc cgagcgcgat gagagatgcc agaagcagca ggctgatgac
     3421 gtttccgttc atgaagtgca gtatgacgag ccggatagtt atacctggcg ttgacgacga
     3481 attttttcga agtttcgcgt ccggcgagcc tcgcgtctgc cgtaaggctg ccctggcagt
     3541 tcctccttca gagcggcatc tctttgatgc gcctctcctc gagatctgca cggtgcccaa
     3601 cggggtccgg agccgcaccg cacgcctgaa cgccagcgat caggcacccc gtgaccgacc
     3661 ggccacccgc gggggtccga acggcaaatc cggctgcagt tccgggcagg cgctttcctc
     3721 cgtctctccc ggagatcgtc tccgggagat gagatcaggg atattccgaa tggtccggaa
     3781 aagatcgaac ataaaataaa gaaggccagg gccgagattc gaacccgggt cgagggatcc
     3841 acagtccctt aggataaccg actaccccac cctggcaagg tactcattat agttggtagt
     3901 cggatttgat taaggtatct cttaatcacg tccgcatccc gaagaccgaa ggtcttctca
     3961 gaccccgtac cgccggaggc agccggctgc cggcgacccc ggccggccgt gtgtccgcga
     4021 tccccgggac gatgtgagca ccgcaggaaa tggtgtccct cccggaacgg agttcgagca
     4081 ccgcaggtgc gaacatccgg agcttgagga acccggaagg tttcgaagac gggagcatga
     4141 gatgactgaa gatcttcgag gaacgacgga tggaacatcc agagtttgag caccgaaggt
     4201 gtgaatcggt gaggtgaatt attaatagac tccatgctct ataacgtacc agtgttccgg
     4261 gcgccgagcg aagcccccgg gggtagggga atcgcccaga atatggtgtg attcggaaaa
     4321 agcgaccaat attccagatt ttttgggttt attcgtccat acttcatata ccgaaaggtg
     4381 attatcatgg caaagcagtc aacgatcagg acccccatcg tctgcgtgat gggccacgta
     4441 gaccacggca agacatcgct cctggacagg atccggggct cgtccgtagt atccaccgag
     4501 aagggcgcga ttacgcagca catcggtgcc acgctcgtcc ccatcgacgc gataaccagt
     4561 atgggcggag ccttgagcca gatcagcgtc aacgttccgg gcctcctctt catcgatact
     4621 cccgggcacc acgccttcac caccctccgt gcgcgcggcg gggcgctcgc cgacatggcg
     4681 atcgtcgtag tggacatcaa cgagggtttc cgcccccaga cgatcgaggc gctccagatc
     4741 ctgcgcaact ataagacacc gttcgtcatc gcggcgaaca aagtcgaccg catccacgga
     4801 tggcgcgtcc agaagggcca gccgttcaag aagacgtttt cgcagcagaa cgagcgcgtc
     4861 cagggcatgg tcgagacgaa ggtctacgaa ctcgtcggca aactctccga cctcgggttc
     4921 aactccgagc ggttcgaccg ggtctcggac tttgcgcgga acatctgcat cgtgccgacg
     4981 agcgccatca ccggcgaggg ccttcccgac atcctcatgg tgctgatcgg gctcgcccag
     5041 cgttacatga ccgagaacct caaggtcagc gccgacggcc ccggcgccgg cacggtgctc
     5101 gaggtgaagg aggagcgggg gctcgggctg acgctcgacg tgatcctcta tgacggcacc
     5161 ctcaaggtcg gggacgagat cgtcgtcgca gggaacgacc agatcatcga ggccaaagtg
     5221 cgttctctct taaaaccccg ccctatgagc gagatcctga tcgaggagcg gttcgagcgg
     5281 gtcaagtccg tcaccgccgc tgcgggtatc aaggtcgccg ccccgaaact cgacggggtc
     5341 atcgcgggct cccctctccg ggtcatccgg ggtgggaacc gggacgagat gatcgaacgg
     5401 gtccgccacg aggtccagga catccaggtg aacctctccg atatcggggt catcatcagg
     5461 gcggacacca tcggcgccct cgaagcgctc tccaaggaac ttgaaggtca ccagatccag
     5521 gtgatgcggg ccacggtcgg gccggtcacc cgtcacgaca tcatcgaggc ggggacgatc
     5581 aaagaccccc tctacagcgc gatcattgcc ttcaacaccc cggtcctgcc ggacgcggtc
     5641 gacaccctgg cggacacttc gatgtcccat gtcagcatgt tcgagggcgg ggtcatctac
     5701 cagctcctcg acgactacgt cgaatggcgc gaggcgaaga agcaggagct cgagcggcag
     5761 cagttcgaga aactggtcat gcccgcaaag ataaggatcc tccccaactg cgtcttccgg
     5821 cagagcaacc cggcagtggt cggcgtccgg atcctcgggg ggaaactcca gagcggtgtc
     5881 gacctggccc tcccgaacgg caagaaggtg ggccgcatca agcagatcca ggcgaagaac
     5941 gagaccgtcc aggaagcgga ggcaggcaag gaggtggcga tctccatcga ggggccgacg
     6001 gtcggccgtc agataaacgt cgacgacgac ctctacgtgg acataccgga gcggcacgtg
     6061 aaggtgatcg agcgggagat gatcaagcag ttaagcccga gcctgcggga gaccctcgag
     6121 gagttcacca tcctcaggcg ccggcaggac cccttctggg ggaagtgaac tgccctgctc
     6181 ccgggcgcgg gcctctcccg gctatcattc ctcttcagcc ccgtaaaggt aggcttttaa
     6241 taccatattc tcaaattgta taggtacttt cgaaggagtc gttaacatgg tagatttcaa
     6301 aattatcctc tcagaaccag aaaccgggcg ctcatataag gttgatgcga ccggccccgc
     6361 ggcaggagcg ctcatcggca agcgcatcgg tgaagagatc gacgggggcg tcctcggcct
     6421 taccggctac acgatcaaga tcaccggtgg cacggacaag accggtatcc cggcacgccg
     6481 cgaccttccc ggacccgcac ggaggagact tctcctctct gagggcgtcg ggttccaccc
     6541 ggtcatggac ggggagcgcc gcagaaagtc ggtgcggggc aacgagatca gcgccgattt
     6601 cgtccagatc aacgccgccg tgaagacgag cggtgcaaaa cccttagccg aatacttcag
     6661 caagcccgag gcggcggctg aataaatcag cacccatctt tttctcgaga tctgtttttc
     6721 ctggaggggc tccgggacga tccaccctgc atctccgttc tttgttctac cgaccgcgcc
     6781 agggttctct gagcgtggcg gggcagcggt cgcggagggg gcatgccccg cagtcacggg
     6841 gatgggtggg acacccgcac gccgcggcat acgcagcggt gagcgccgct ctcggctctc
     6901 ctccgtatgc agcggtaagc cgtgctgccc cggcggcgac ctcatccggc accgtctcgg
     6961 ggtagtaacc gctccaggcc acgtcctcga gtcccatgtc acggcagacc gagcagaggc
     7021 gttccatctg ctccttcgac ggccccggat ggtaatagag aacgttattt ggccgaaacc
     7081 cgatgagccg gtagggcacg cccgggtcga gcgaggcgat gaacgccgcg acggcaccca
     7141 cctccccgtc ggtcatgccg gggatcacca ccgtccgaaa taccctgatg cggtctctcc
     7201 cctcgtgcac gaggtactcc gcgttcctga gcaccggacc taccggcgct cccgtgaggg
     7261 cgcgatggac cgggtccgac catgccttga tctcaagggt gatcacccgg cagaggtcga
     7321 cgatccgctc catcgtcgcc tctgttgcaa agccgccggt cgagatcccg atcccgagat
     7381 cgagcccctg ccgcttcacc tcggccgcca cttcttcgat gtaggggagg tgaatgatcg
     7441 ggtccccgcc cgtgaagccg atcgtccgga tacgccggcc cctgtaggcg gcaatccgct
     7501 cacgggcttc ggccaccagc accgctgccg gcacgtaacc ggcgtactgc cagccggggt
     7561 cggggtactg tgagatccgg taggcgttgc agtggaggca gcggtagcag catccgagca
     7621 gggtgacgat gtaactcgcg agggatcccg aacacatcgt cgccgccacc tccgcacggc
     7681 cgacacggca taccccccgc tctccccgga ggcggtcgac gctgcatctc cactcgcaga
     7741 ggcggcagga ctcgaggtct ttcataaaaa tcaggcagga gacccccggt ttcaaccggg
     7801 g
//
LOCUS       DAJB01000217            6253 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_116567, whole genome shotgun sequence.
ACCESSION   DAJB01000217 DAJB01000000
VERSION     DAJB01000217.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_116567"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    201..348
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1019 a         2055 c         2064 g          967 t
ORIGIN      
        1 agaagtagta ggtcttgccc ttgtattctg ccgtgaactt cgcggtcgcc tcatcgacgt
       61 ccatcttgca taccggatca actgccatgt gcatcaacct cccgatagca ttcctctctt
      121 cgccggaggt atatatgtct tgagcagcag ggagagcgag acgaccgtca ccgacgagag
      181 cgccatcgcg agcgccgcaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gttgatcccg
      361 tcgcccacga acgccacgac ctcgccccgg ctctggaggg cccggacctc ctgcgccttc
      421 tcctgcggca gcaccccggc gtggacgtcc tcgatcccga cctcacgggc gatggcgttt
      481 gccgtccgcg ggttgtcgcc cgtgatcatc gtcaccgaaa ggcccatccg ctttaactcc
      541 gcaacggcgc tcctcgtcgt cggcttgagg gtatcggcga tggcgaggat acccgcgaac
      601 tcagccccgg cggcgaccag gacggcggtc ttccccacgt cctggaaggc ggctatcctc
      661 ctctccgccc ccgccgagat agcgacgccg tgctccagaa ggaacggctg gttcccgatc
      721 agcacctccc tgccctcgac cgttgcgctg acccccctgc cgccgaaggt cgtgaaccgc
      781 tccgactcag ggatgctgac gccggcgctc tccgcccgcc gcacgaccgc cgccgcgagc
      841 gggtgctggg agttgtgctc gaccgccgcc gcgaccgcga ggagccggtc ctccggcatc
      901 ccgaacgcga cgagatcggt gacgtccggc tttccccggg tgagcgtccc ggtcttgtcg
      961 aagatgaccg cagtcagctt ctcggagatc tcgagcgcct cgccgttccg gatcagcacc
     1021 ccgagttcgg cgccccggcc gatcccgacg gtcacggcgg tcggggtggc gagcccgagc
     1081 gcacaggggc aggcgacgac caggacggag atcagcaccg tgagcgagaa gaggagggtg
     1141 gcgccgagac ccacgtacca gacgaggaag gcggcggctg cgatcgcgag cacgaccggg
     1201 atgaagtagg agaccgcgac atcggcgatc cgctccaccg gcggctttga accctgggcg
     1261 tcccggacga gcctgatgat ctgcgagagc accatatcct tcccgatctt ctcggcccgg
     1321 acccggagca cgccgttcac gttcagggtg ccgccgacga ccccgtcgcc ctccttttta
     1381 tcggccggga tgggctcgcc ggtgatcatc gactcatcga ccgagctctc gccgcccacg
     1441 accgccccgt ccaccggcac cttctccccc ggccggacca ggagaacgtc gccgacgacc
     1501 acgtcctcga tagagacctc gacctcttga ccgtcccgga tcaccgtcgc ggtcttcggg
     1561 cggagtccta tcaggctctt gatcgcctcc gacgtcctcc ccttggcccg ggcctccagg
     1621 tatcgcccga gcgtcaggaa tgaggcgagc atcaccgcgg tgtcgtagaa attaaaatcc
     1681 ggggtgagga caagcccgaa ggtcccgagg atgcttgcgc cgtaagcgac gccgatgccc
     1741 atcgcgtaca tcacgtccat cgtcagcgcc cggttccgga gcgcggcggc ggccgcctta
     1801 aagatggggg cgctgacgta gagaaagacc gggagggtga tgacgagcat gacaaggttg
     1861 accgggaccg ggagggcagc catccccggc atcccaaaga gcatgtagag gaagagcggg
     1921 atgctcaccg cgaacccgag ggtgaaccgc cggaacttgt cccggaggtc ttcctcgcgc
     1981 atcctggcct cgacgtcttc ggagacctcc tcctcgagcc ccaggtactg gtagccggca
     2041 tcctcgacgg cggcccggat atccgatgtg gtgacgagcg ccgggttgta gacgacgtgt
     2101 gcgttctcgg tcgcaaggtt gacccgggcc tcgtagaccc cgtcgaggtc ggcgagcgag
     2161 gcctcgatca cctgggcgca gcttgcgcag accattcccc cgatcctgac ggcgacctcg
     2221 ttcctgataa cctcataccc ggcctcgccg accgtctgtt cgaggtctgc gagggtggcc
     2281 ttcgtcgggt cgtactcgac gacggcggtc tcggccgcga ggttgacgcg ggcctcgtag
     2341 acgtcgtccc ggccctggag ggcgcgttct atattgaggg cgcaggacgc gcagtgcatc
     2401 ccggagatct tcagttccgc cttcctcttc tcctccggca tgatattctt ctcgctgcgc
     2461 ggggataaat acctgtaact ccggcccgtg gttcccggtg caaccacgtc cggatccccc
     2521 aaattacatc tgccgacttt tatatacgca atattattat actcgggggc aggtataaac
     2581 gccctatcca gaggtgattg tggtgcagag aagggatctt ccaacagagg aatcgcaggg
     2641 agagaagcga tacgatgttg agcgtatggg atcgagcagg gatatcgagg gcctgacaga
     2701 agcgctgcgg agcagcgatc ccgcagttcg ccggagcgcc gccctggggc ttggcgggct
     2761 cggggaatgg agagcggtgg aacccctcat ccatgcgctc gccgacccgg tgcaggccgt
     2821 ccgggaggga gcggcgaacg cgcttgtcat ggtcgggacg ccggcggtcg aaccgctgat
     2881 cgacctcctc gagcgccctg gtgctgcagg tgtgtatggg gcgcccgggg agggcctcac
     2941 ccagttcgac ctcctcgccg gccctgaggg cataccgctt gcaaaaagga aactccggca
     3001 ccgggacggt atcacccagc acgatgctct cggcggcccg gaagatctca agaaggcggg
     3061 aggcagagga gctctcgatg aagaagagga cctctcccag cacgacgcgc tcggcggccc
     3121 ggaggatatc aggagggcgg ggggcagaag gtcccttgat gaagaagggg agatcaccca
     3181 gcacgacctt ctcggcgggc cggaggatgt cgggacgaag aagaccctcc ggcaccggga
     3241 gattgcgcag cacgacctct ttggcggtcc ggagggtgtg agggagcacg acgtgctctt
     3301 cccgggggcg aggagggccg gcatcgtgcc ggagggtgct cttcccggtc ggggggtgcg
     3361 ccgggcctac gccgccgtca tcctcggcga gatcgccgac ccgcgggcgg agggaactct
     3421 cggccgggtg ctcaacgaca gcgaccccgc cgtccggagg gcggccgagg acgggatggc
     3481 gcggtaccgc gagaggcgag gtatggccac gcccgtcccc ccggcgtccc gctgatgccg
     3541 cggacggaca attctctttt tccgtgccgc ggacggcgat cccctgctgc cgcgatgcgg
     3601 tcgggccacc gtatcagcaa gattattata tcgagcgggc gccatacacc agaaccggag
     3661 gcgaagaagg ttgcagcaag agagtagtag tccgggtgag aggcggttca atatcgatct
     3721 cctgagggcg gacgaggaca tcgacggcct gatcgaggcg ttaagaagcg ataacggcct
     3781 gacccgccag cgggccgccc tggcgctcgg ggacctcggc gaggcgaggg cgacggagcc
     3841 gctcatccgg gcgctcggcg acccgatgac ggcggtgcgg gaagcggcgg cggattcgct
     3901 tgccatgctc gggagcgcgg cggtcgggcc gctggtcgaa ctcatcgaga gcccggaggc
     3961 gtcggggagg tacgaggagc ccggggctcc cggaggcacg gtgaccgtaa ccggacccgg
     4021 cggccagacc tgggagatcg agacccgaag agacctccgg cgtgtctacg cggccgccat
     4081 cctcggggag atcggcgatc ccgccgcggt gaagccactc gtcggagcgc tccgggacgc
     4141 gagcgacgac ctccggtgcc atgcggcggg agcgctcgcg aagttcggga gcgaagcggt
     4201 cgagccgctc acggcgatgc tcgccgaccc ggacccggag gtccggatcg tcgcggccgg
     4261 ggtcctcggc gataccagcg atccggctgc ggtggagccc ctcgtcgcgg cgctccgcga
     4321 caagaacccc gatgtccggg gggcagcagg gggcgccctc ttccgggtag gcgatgccgc
     4381 ggtcgagccg ctgatcgcgg cgacgaaaga cgccgaccgg aacgtccggc tctacgcggc
     4441 gggggcgctc aagtacatcg gcaacccgcg ggcgatcgat gcgttgcagg agctcgcccg
     4501 gagcggggac acggaggaga gaagcgtcgc ggaggacgcg atcgagaaga tccggatggt
     4561 ccggggggag gctgcgccgg agccttcggc gccgacctcc cgataatcca tcttactttt
     4621 tccggaacga ccggagcagg accgctccgg tctcgaagat ttctggtttc ccttatcccc
     4681 ttctcctcac ccttaccgac tccgcgtggg cgtggagccc ttccgcatcg gcgatcgtct
     4741 ccaccacgtc gccgattgcc tcgaggccct cccgcgtgat catctggacc gtcgagcgcc
     4801 ggcagaagtg gttcacgtcg agaccggagt agaggctggc gtagcctgcc gtcgggagga
     4861 cgtggttcgt ccccgacgcg tagtcgccgc aggccaccgc cgcgtagggg ccgacgaata
     4921 tcgatccggc gttccggatc cggttgagga tgccgagcgg gtcggccacc tgcaacgaga
     4981 gatgctcggg ggcgatgccg tcgaccgtct ccactgcctc gtcgaggtcg ccggccacga
     5041 tgtagccgga gtggccgagc gccttctcca cgatctcacg ccgggccgcg cccgctgcca
     5101 tccgcttcac ctgctccgtg accgcatcgg cgagggccgc atcggtggtc accaggacgc
     5161 aggcggcgtt cgggtcgtgc tcggcctggg cgaggatgtc ggccgcgatg aagaccgggt
     5221 tcgccgtgct gtctgcgagg atcgcgatct cgctcgggcc cgcggggaag tcgatctcgg
     5281 cctcgttgcg gaggagcatc ttcgcggcgg tgacgtagac gttcccgggt ccgacgatct
     5341 tttccacccg cgggatcgta tcggtcccga ttgccatcgc cgcgatcgcc tgggcgccgc
     5401 cgacccggta gatctcgtcg accccggcga tatcgagcgc gacaagcgtg atcgggctcg
     5461 tcggcggcgg ggtgcagcag cagatctccg gaacgccggc gatgatcgcc gggatcgtgc
     5521 acatcagcgc cgtcgagggg tatgccgccc gcccgccggg gacataggcc cccacccggg
     5581 ataagggggt cgtcttgacg ccgagggtga tccccggctc catctcctgc agccagaggt
     5641 ctcgcccgcg ctgcagttcg tggaaccggc tgatccgcgc ctcggcctcc acgaggctct
     5701 ccacgagctg cgcatccacc tggtcgtagg ccgccacccg ctcctcgtcg gagacggcga
     5761 tctcctcgag atcgatgccg tcgaacttcc tcgtcaggtc gaccagggcc gcgtcgcctt
     5821 ccgcccgcac cctcccgatg atctcaaaga ccggtccctt cacccggtca aggtccgacc
     5881 gccgcccggc gatccaggtc tcgatatcca gcgcctgcca catgagggaa atggttggcg
     5941 ggccggagcg ttaaggtttc gaccccactg gcaaccagcc cccggaatgg agcccgttaa
     6001 cattccggcc aggataacta tatatccgag gaaccccccc ctagcgcgtt gctatgaaga
     6061 cgaatgcgac tcttctgacg gcaggcatca tcacgatcct gctcatcctc gccctctgtg
     6121 ccgggtgcac gagtccggcg ccgggcggaa acgagacgcc ggccggcacg gcgacgaccg
     6181 ctcccgccgg cgggggaaac atcctgcgga tagccaccac gacgagcctc gagaacaccg
     6241 ggctgctcgc gga
//
LOCUS       DAJB01000218           10749 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_28224, whole genome shotgun sequence.
ACCESSION   DAJB01000218 DAJB01000000
VERSION     DAJB01000218.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_28224"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    3092..3119
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2262 a         3204 c         3105 g         2148 t
ORIGIN      
        1 acggcctcgt gggatcgctc gtccttatct acctgacata cttaaacacg atcaccaaca
       61 cccagtactt ctcgttcttc ggcgggcttg ctgccgtggt cgcactcctc tggggtaccg
      121 acacgatcaa gcacctctgc agttacggtc ttggtaccgg tgtcccgtcg gcgggtatga
      181 tcgccctcgg gtccggtgtc gtcgccgcgg tcttcggagc cacgaccggc atcttcgcgc
      241 ccatcgtgac gataattatc gctgcgatca tcggtgcggt ggccggagta ctggcgaaca
      301 acgtggtccg gatggacatc ccggtcatga ttgtctccct gaccgaactt gcgatcgtcg
      361 gcgcgatgac cgtgctcggc ctcgcagcaa tggcctgcgg aacgttcgaa ttcctcggca
      421 tgatcaccgg cacggccgtg atccttggat tcgccgtacc gagctacgaa ttctccgtca
      481 tcggcggcag cattatcgcc gtgatcttca tgcttggcgc catcgccatc cagcactcct
      541 tcaacgcctg tctcgggcca ggggagaagc aggaccggac actcatgctc gccgcagagt
      601 gcggcttcct ctccatgatc cccgtcgcga tcatctcgtt cgcgttcatc gggttccttc
      661 ccgctctcgt cgcactgttg gtctccatca tcggatggat ctacacctac gtgcagtaca
      721 tcgcgctctc gaagcgtgac gcctacgcat ggctcgacgc gaagccgatc ctggagccca
      781 agggaggtgc ctaaatggca tacattcagg tactgcctga gttcggactg gtcgtcgacc
      841 cgacggttgg catcatcacc accgccggtg tctcgtacac ccccgtgctc gagcaggtag
      901 cagaactcga gaagatcacc gacgacctgg tcggcatgct ctccggagag ggcagcttcc
      961 tggcatcgtt cccgaacagg gagggagttc tcaacgtcgc cggaggcgtg aacgcattct
     1021 ggtacggcat ggcgatcggc cttctgattg ccggtgtcgt agtattagga ctgctgtgag
     1081 gtgaagaaca tggttgagaa gaaatcaccg gccagtggat ggccgattgc taagggcgac
     1141 ttccacacgg gagatgcgca gagttgcgtc gccgtcatca ccatgggatc ccacctcgac
     1201 gaacagggca tcgcagatgc cggagcggca atcgccgggt cctgtaagac cgagaacctc
     1261 ggcctcgaga aggtcatcgc aaacgtcatc tccaacccca acatcagata cattctctgt
     1321 tgtggtacgg aggttaaggg gcacctctcc ggtcagagct tcattgcgct gcatgccggt
     1381 ggagtctccg gcggcaagat cgtcggagcc cagggagcca tcccgttcat cgagaacctt
     1441 accgacgatg cgatcaagcg cttccaggac caggtcgaga tcgtcaacat catggaaagc
     1501 gaggacatgg gcaccatcaa ggccaagatc aacgaactca aggccaaaga tcccggtgcg
     1561 ttcggcgcag aacccatgat cgtcgaggtc aaggaagcag gcggtgcggc agaggaagcg
     1621 actggcgaag tacagccgct cactggcgaa ctggcactga tacatgcacg gatgaaggtc
     1681 atcgagcgga tggtcaccga catcggctac cgcaacaagt ttgccgccgg cgtttactcg
     1741 ggcaagatcg agggcctcat gatcggcctg atcgtctcgt tcgtgattct ggggttcatc
     1801 ctgctggggt gagatagatg gcagaagaaa gtacacaggc agcaggcccc atccggatga
     1861 cctcgatcaa cagaatgatg gacgacatcc ggtacaaggc acagatcctt gcccgcacga
     1921 ccaaactcga gtcaggcatc atgggcatgg gaatcgtcgg gttcgcagtc gggctcggca
     1981 tcatcatgct ctgcgtcctg gttccagctc tgatgctggg ggcgatctaa catggttgag
     2041 aagaaatcac cggccagcgg atggccgatt gctaaaggcg acttccacac gggagatgcg
     2101 cagagttgcg tcgccgtcat caccatggga tcccacctcg acgaacaggg cattgcggat
     2161 gccggagcgg caatcgccgg gtcctgtaag accgagaacc tcggtctcga gaaggtcatc
     2221 gcaaacgtca tctccaaccc caacatcagg ttcatcctct gctgtggtac ggaggttaag
     2281 gggcacctct ccggtcagag cttcattgcg ctgcatgccg gcggagtctc cggcggtaag
     2341 atcgtcggag cccagggagc catcccgttc atcgagaacc ttaccgacga tgcgatcaag
     2401 cgcttccagg accaggtcga gatcgtcaac atcatggaaa gcgaggacat gggcaccatc
     2461 aaggccaaga tcaatgagct caaggccaaa gatcccggtg cgttcggcgc agaacccatg
     2521 atcgttgagg tcaaggaagc aggcggcgcg gcagaggttg ccgctgcagg cgcaaaccca
     2581 cagttccttg agatcgaaga gcggctcaac gctatcgagg agaggatcga gttcgtcgat
     2641 gcagagatag cccagcgcgt cggacgaaaa gtcgggcgcg acatcggcat cctgtacgga
     2701 ctggttgcag gtttgattgt attcatgatg ttgttggtat tactgcccaa attggttggg
     2761 tacctgtaag gaggattgac gaatatgttc aagttcgaaa aagagcagac ggtacacgac
     2821 ttcaacggta ccatgatcgg cgggcagcct ggagagtacc ccaccgtgct cggtgcatcc
     2881 atcttctaca acaaacacga agttgttctg gatgaccaca ccggaaagat cgacaagctg
     2941 aaggcagagg cactctggaa ccgctgccgg gaactctcgg atatcaccgg cattccgcac
     3001 ttcattcaga tcatcgcgga atacggcgag gccttcgaga gctacatcga ttggttctgt
     3061 ggaatcgacg acaagaccgc gttcctgatg gnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3121 agaacatcga agcgctggcc aagagcgatg tgacagcagc catcgtcctc gccttcaacc
     3181 ccgccgaccc gtcggtcacc ggccgtgaga aggttctggt cgagggcggc gtagccggac
     3241 aggaactcgg catgctcgcg atcgcagaga agtgcggcat cacccgtccc atcctcgata
     3301 ccgcagcaac ccccctcggt ctcggctccg gtggttcgta ccgtgagatc cttgcctgca
     3361 aggcgatcca cggcctgccg accggtggtg cttaccacaa catgacggtc tcctggacct
     3421 ggctgaagcg gtggaaggga accaagaaga acccgtccca gcagcttgcc ggcctcgagg
     3481 gcaaagatgc ccttatcgag cagctcaccc accactatct cggtggcgtg gatggaatgc
     3541 gccaggcggc ctggtctgca cccgatatcg gatgcaacat ggttgcaagc acgctcggtg
     3601 ccgacctgat catgtacggc ccgatcgaga atgtcgaggc gatgatcacc gcacaggcct
     3661 acgtcgatat cgttgtgctg gaagcggtgc gcgacctcgg tatcgagccc nnttaccgtg
     3721 attattccgg ggacacgcct gtcaggcgta agtcatacaa gtatcaggtg caaccggtac
     3781 cccggggctc atcgtgtgcc cgtggaaggg cagcagagac accagcctca ggagtgcgag
     3841 caccgaaggt gtgctgcctt aatggctgat acgcaggtga tcccttactt gagaactttc
     3901 aggaggctcg tatgacgagc aggattccac aaaacaggtg ccatcacagc ccacgatatg
     3961 cactacggcg gtgacgtaca tagagaatta caacctcccg ctcctcatga tgcacggcat
     4021 atataatccg gtaatctcct acccggatcc ggtagaggtg ctctgctccg cttaacttac
     4081 gtacaccaaa cggtgaaggg ttttcagata gaacttgtat gctctgaaaa atgcgcaaaa
     4141 caacatcgcg cggcaatggc gcaatatctt tttcgatact cgccttaatg cggatcgtgt
     4201 agcccgccat tactactgct cgtaccgctt ccggaactca ttgaacccta cagtcggttc
     4261 ttcacgccgc tccgcaacca cggcaaggtc atggagatct tcctgcagct gctcgtactc
     4321 gcagagaggg agaataatcg cgacccggtt cccgtgctca tcgacgacat actgttctcc
     4381 tgtagcactc atcgttctcc tcacttgtag atggagcgca agaaccatta atcttctttt
     4441 tacgatacca ggcgaagggt atcgccagcc gtattaccga agcgtcgctc ctatgacatt
     4501 tgagtccctg cgtctccggc aggacggtcg cgctccgatc ggtcatcctt gcatcacaaa
     4561 aagaagcgtt tgggctctgt agaaaaccct gtcggaggat cacaccgcct ctccacgtca
     4621 actgagcagt acccggggtt ggttgaagca gttctcttca gtcgcacata gatctcgact
     4681 cacgcgaaga cgcgaagaac ggttggtgag cattgtatcc cttcgcgtct tcgcgcgata
     4741 tcacatcact gatttctaca gaggcagcgt ttgttattcg gaaggcgtgg aaccccccac
     4801 cccaatagct cacaccagtt cgatatgccc ggcaatccgg agtttccccc cctcaaggta
     4861 gtggagcacc gccgtatcgc cggggaggta gacgaggtct ttttccagcg caagcgtgag
     4921 cgtcggttgc cgccagttcc cgtcatcccg taccgcctcc acccgcgccg ggatgaactg
     4981 catccagtgg ccgatgtgga gcaccgtccc tgccgtgagc gctgccggcc agtatttgac
     5041 cagcgtcgcc cgggcctcaa gggtcgtccg ggtccggatg gcaggatcgt ctgagaggac
     5101 aaagccccgg tcgagatcgt cggactcgat gttcttgagc gcaagcccca cgcggtcgcc
     5161 ctcggcggcc cagtcgaagt cgtcgtcatg cttctggatg gaccgcacca tgatcgcctg
     5221 ctccccggga tagatcttca gggcatcgtg cttctttata cccccccgca ccaccccgcc
     5281 gagaatgacc gtcccgatgc cgcggacgtt gaaatggtga tcgatgggga tcgttcccac
     5341 agcaccggcg ccgggctgga cggagggccg ggcgtgtgcc tcgcggagca ggcggtcgca
     5401 cagcacgatc gggtcagcgt ctacgaactc gtagtgctcg agcaccgttc cgcggaggag
     5461 cggcgctatc tgctcaggcg ttacgtagtt tttgaggatg atgcaaccct tttctacccc
     5521 cacttcatcg agcatcagca cccactcccc gagcatggga gtgatctcac tcaccacgat
     5581 gagaaccgta tccgccatcg atgcggcgta gaagagcggg gccagccgct caggataccg
     5641 cgtaggctcg atgatggtga cggtatcgtc gcccttcttc aggttataga aggtgatatc
     5701 ggattccgtg cccttcttcc cgaggtcctt cgcgtaccct gcgggtccca gcacggcaac
     5761 attcagattg cccatatggc agagaggttt gacggctcat cgtatcaggc ttacccagat
     5821 tccctccgat gcacaagaat ccggtcaatc tacgtttcta gaaacctggc gcgcggcgat
     5881 acacaagatc ggtttaataa agtaaaaact actttattta atggagaatg tccagaatca
     5941 ggttgattgc gattccgata ctcatttgcg tccttcttgc cagcatggtt cttgccgtgg
     6001 ccgtcgggcc aaccagcgtc tcgatcgcgt cgctcttcag ttcggagacg gcagggatga
     6061 tcgtccggga cgtcaggctt ccacgggtga tcgctgcagc gctggtcggt tgcgcgcttg
     6121 ccgtggcggg cacagccatg caggggctgt ttaagaactc catggcagat ccctatatca
     6181 tcggcacgtc aaccggcgga tctctcgggg ccacgctatc catcgtcttc ttcgcaggtg
     6241 ctggattgcc ggttttcgca ttcgcagggg cgacaatcac tacttttgcc gtctacacca
     6301 tcgcccgcca gggcgggaag attcccgtcg agacgctcct gctctccggc gttgccctct
     6361 ccatgctcct ctccgcagtt ctctcgttcc tcatgtatac ctccggacgg agcctgcatc
     6421 agatcatgtt ctggctgatg ggagggttct ggaatgtcgc atgggacgac gtcttcatcg
     6481 ccatcctcat cccgatcggg tgcgtggcca tctacctcta tgcacgcgat atcaacatca
     6541 tatcgctcgg agaagaggac gccatccatc tcggcgtgaa tgtcgaacga ttaaagcgga
     6601 tcctcctctt tgcgagcgcc ttcctgacgg gcatagcggt ctctatcgcc ggggcaatcg
     6661 ggttcatcgg cctgatcact ccccacgtga tgcgcctgat cgtcgggccg gaccatcgca
     6721 tcctctttcc tgcggccgcc cttgcaggcg ggatcctcct cgtctggtcc gatacgctcg
     6781 caaggacgtt tgccggcgac atgccggtcg ggatcgtcac cgcctgcttc ggcgcaccgt
     6841 tcttcatata ccttctgcgg agtcggatga aagcatgaaa ccggtcgaga tcatcgatat
     6901 cgacgtctct tacggcgcaa agaagatcct cgaggcgatc tcgttttatg cggacagagg
     6961 cgagatcctc gggatcatcg ggccgaatgg gtccgggaag acgaccctgc tcaaagcgat
     7021 gagccgggtt gtcgccccgg attcgggaga ggtccgtctc tccgaacagg gcctgggtac
     7081 cttcagtttc cgggaactgg cgcagcaggt cgccgtcgtg ccgcaggaga tctcaatcag
     7141 cttcgattat accgttcgcg agatcgtcat gatgggaagg cacccctaca tcgggagatt
     7201 cgcttccgag accgcccggg atctggagat ctgcgaccgt gccatgcacc ttgcaaacgt
     7261 cacccgcctt gcaggttcat cggtccacga gatcagcggg ggtgaacgac agcgggtgct
     7321 catcgcacgg gcgctcgcgc agaacccgaa ggtcttgctg ctcgatgaag caacctcgaa
     7381 tctcgatatc agtcatcaga tcgagatact gaacatcatc aaaggcctca ccgtcgatat
     7441 cacagtgatc agcgtctttc atgacctgaa cctggctgca tactactgcg accgtctgat
     7501 catgctcaaa gaccggaggg tctatgccgc agggccgccg ggggatgtcc tgacccggga
     7561 gacgctcaat gtggtatacg ggatcgacgc actggttaaa cctcatccgt tgacagggaa
     7621 gccgtatatt ctcccaacat acgaatacgc cgcaaaacac catccggaac ggggcgtgca
     7681 cgtcatctgc ggcggcggca cgggatccga tctcctctac gccctccaca cagaagggtt
     7741 tctggtgagt gccggcgtgc tcacggtctt cgataccgac catgcaaccg caacagaatt
     7801 aggcatcccc tgcgttaccg aggcgccgtt cagcgcgatc tccccggatg cctgtagtga
     7861 actcaggcgg tgcatcgacg ctgcgcatgc cgtcgtcgtc acggcaatgc ccatcggacg
     7921 gggcaacatc gagaacatca gggtactgga aaattaccgg aacaagccaa tcatactcct
     7981 gaacgacggc gggcaccggt ccttccagga ctgtaccgga ggcgaggccg aagcgatcat
     8041 tcacgggctg cttgaacggg gggcggtcac ggcggagcgg attgaggagg tcctgcagat
     8101 cctcagggag gcagtgtgac ccgccgctgc agaatcgcag gtaccgcaga atccgctgca
     8161 ttttcacagg ataccgtgat cgctgccgct gctacagtac caacccggga actcactcca
     8221 gcagcgacag aaatcaccgg gagaaccatg acggtgaccg acgactccgg ggagaccgtc
     8281 accatcaggg gaataccaca gagaatcatt tcccttgctc cctcgaacac cgagatcctg
     8341 tatgccctcg atctggggaa ccagattgcc ggtgttgccg acaacagcaa ttaccccccg
     8401 gaagccaccg ataaaccgaa gatcggcggg tacagcagta tcagtatcga gaaggtgatc
     8461 gcggcggaac ccgacttgat cgtcgctgcg tccggcaaca cggatgaggt gattgaccac
     8521 ctgcgatcgc tcgggatgac cgtcatcctc ctcgacccac agacgatcga cgacgtcctg
     8581 cgggagatcg aacttctcgg agcgatcacc gggcaggaag agcaggcagc gacgtgtgtt
     8641 cgggaactgc aggaccgcat ccgggcagtg actgatacaa cggaggctct ggcggagagg
     8701 ccctcggtcg cccatgtcgt ctggcacgat ccaatctggg tcagtggcag ggggaggttc
     8761 ctcgatgaag tgatcacgct ggccggggga accaacgcat tcggctcggt tgatgaatgg
     8821 agcatcgtcg gctttgaaga gttcgtcgcc gcggatccag agtatatctt cgtcagctcg
     8881 ggcaccggca tgaccgaggg ggggtgcgac atcatctacg actacttcat gaacgagccc
     8941 cggatgcaac ggctggaagc ggtcaggaac ggacatgtct acgtcgtcga cgccgatatc
     9001 atcagccgcg ggagcccgcg gattgtggat gcgctcgaag aggtgggggg ggatctccac
     9061 ccggacctct tcgggacaga cgccccaatt gcaactcctg cggtccaatc tccgggctcc
     9121 ggtgggatgg cactcaccgg tgcgttgttc atcgatttcc tgctcagaac aaagagataa
     9181 accagggacg atgcgaattc tgacagtatt ccaatagttt ttgtggaaca ggcgcggcgc
     9241 gcggctctgc ataccgggtg cctctccggg gataaacttg atgcgcggcg ccacccgggt
     9301 caacgggtta ggcgatctca tgtcccgtct ttcctcgaca taacgccgcc cgatcaggcc
     9361 agaaacagcc ttaaaaaagc gtaccggcac cggaacgccc ggtggaagtc cgaccggtat
     9421 ccacaggtta tttttagtta ggacaaatca ggattataaa attttattaa cttaaacaaa
     9481 gtgtacttgt atttatgaaa ccgaaactgt ttccactctc cattgggctt atcatgctct
     9541 cactcctgct cgtcggcgtg gtgagcgcat catacccaat gtttcattac aacgggcaac
     9601 gaaccggcta cgtccctgaa gacgggccgc agaacaacac catcctctgg aaggccaacc
     9661 tcggtgagtt catcggcgcg ccgccggcgg tggagggcgg ccgggtctac atcggggtct
     9721 ggccggatat gaactttgct tccggggaag aatattacct cttctgcctg aacgcctcca
     9781 ccggcagcga ggtctggcgg aacccgctgg gtgccggtga aggtaccgta tccggcgcag
     9841 ccgtctccgg cgacaggctc tttgtcggat gcatggacgg gaacctctac tgtatcgata
     9901 ccacggacgg cagcacactc tggaatgttc ccgtcgatac cgggagagaa ggcggcaact
     9961 ggtacggcct ctcctgcagc ccggttgtgt acgacgacat ggtcctggtg acctccatga
    10021 ccgacgggac gctgcatgcc ttcgatcccg atggaaatga ggtccggacg tataccaccg
    10081 gaaacacgac gttcgcctac tcctccccgg ctgtcgatgc cgcaggccgg gcgtacttcg
    10141 ccggcaacgc cgtgaccgat gccctctact gtatcgacat cgataccggt gaggagatct
    10201 ggaacgtcac caccgacggg caggtaaggt cgtcgccggc gatcgacggc agcacggtct
    10261 atgccgcgtc ggcaaaccgg ctctatgccc tcgataccgg cgacgggagc gaggtctgga
    10321 acaggtcggt cagcgcatcg tggggcacgc ctgccgttgc cggcggcagg ctctatctcg
    10381 gcacggccga cggggtcatg cactgctacg atgcacagac cggagaagaa gagtggacgt
    10441 ttactgcaaa cggaaggatc gacgcctccc ccatcgttgc gaacgacacc gtctacttcg
    10501 cctccaatac cgggacagcc acaatctacg cggttgatac tgcaggcaac gagatctggc
    10561 ggtacgccac gaccaactac ctccactcat ccccgtcagt atcggacggc atcctctaca
    10621 tcgggtccga tgaagggagc ctctacgcct tcggggatgg gctggttccg acaccggtat
    10681 tccccggatg gagcggatct gtcagactcg ccgaaggaga gaccttcacg gtcacgccgt
    10741 tcaacaacg
//
LOCUS       DAJB01000219            4123 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_107310, whole genome shotgun sequence.
ACCESSION   DAJB01000219 DAJB01000000
VERSION     DAJB01000219.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_107310"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          768 a         1278 c         1373 g          704 t
ORIGIN      
        1 cgctgccggg cggacgcgat cgggaaactc ggccaggacg ttcagtcctg tgtctaccag
       61 cagatgaagg atgagaaggc agagtaacca actctttttt acctgccggt gccgccggcc
      121 atgacgacga tcacgctcgg accggaccag ccgttcgacc tcgaccggac gctctcctgc
      181 gggcaggcct tccgctggga gaaggcggag ggggggtggc agggcgtcgt cgacggccgg
      241 gccatccgga tacggcagga gggaagcctt ctcgcgttta ccggggcgga tgcggagttc
      301 gtccgcgact acttccggct cgaccaggac ctccccgcca tcctctcgtc gatcgaccgc
      361 gacccggcga tcggcgctgc gatccgcgag tgccggggcc tccgtctcgt caggcaacag
      421 ccgtgggagt gcctggtctc gtacctctgc gccacgaaca cgaacatccc ggcggtgaag
      481 cggcgggtcg cgctcatggc ggaacgctac gggagaccga tagacgggcc gtccggcacg
      541 gcgtatgcgt tcccggagcc ggaggcgctc gcggcgatca cccacacgga cctccgggac
      601 tgcaagctcg gctaccggac ggattacatc tcctgcgccg ccgccttcgt ggcggagcac
      661 cccgactggg cggaacgggt cgccgccctc cccttcgagg aggcacggca ggcactgatg
      721 cactttaagg gcatcgggcc gaaggcggcg gattgcgtgc tgctctttgc gttcgggttc
      781 ctcgaggcgt ttccggtcga tgtctggata caccggatcg tagcggaggc ctacctcccc
      841 gaacttgtcg gaaggagttg caccccggcg gagtacgagc ggatccggcg gtttgcgcgg
      901 gagcacttcg gggaatacgc agggtatgcg caggagtacc tctactgcgc acggggcccg
      961 gcacgccgcc cgcagtgagg ctcacccttt cccgttgcgg atacgctccc ggatgagcaa
     1021 ggctcccgca agcaggatga ggatgccggg gacgacgatg gtgacgaggg caatgggcac
     1081 tccacacacc gagatcacgg gcgaggcgat gccgattgcc acccgtttcc cgggaggcac
     1141 aagcgtcacg ttgtcacaat ccaggtgccg ggacatcggc atactgtaga cgtgcgccct
     1201 caactcaggg ggcatgctct ccaggaattc ggggcagacc tgctcgtagt actcgccgag
     1261 ggatgcgttc tgctccagca tccgttcata tatctctgag tgcggcacgc tgagcgagca
     1321 ctcgatgggg atctccccgc ctcctgtctc ccctgcggcc gatcccggca aaagtgcggc
     1381 aaccgcgagc agtgccagga atagcacgta gccctgcttc ttcatggtcg ctatcagagg
     1441 ggccgggagt ccggcggccc ctgcctatga tcagtgatgg gcacccgggg cataaggtcg
     1501 tcccgaaacc cccggcccgg caaaactcgc gataaacccg ttctcccgca tcgcaagcac
     1561 ctcctccggc gcaccctcgg ccgccacccg cccttcctca atgaggcaga ggcggtcccc
     1621 gagctcgagg gcgtcgcggg gggtgtgggt gacgatgatg cagggtattc cggcattccg
     1681 gatccggtcg cgaagttccg tccgcatcgc agcttgagcc cgcatatcga cggcggcgag
     1741 cggctcgtcg aggaggagga gctccggttc gagcaccagc gcacgggcga gggccacccg
     1801 ctgccgctgc ccccccgaga gccggccgac gttgacgtcc gcaaggtcgg agaggtgcat
     1861 cgccgcgagc tgctccgcca cggtgtcggc tatctccggt cgcgggatct ttcggcaacg
     1921 gaggccgaac gccacgtttc cggcgacggt catgtgcggg aagagggcgt aggactggaa
     1981 gaggtggccg atgttccggc tctccgcggg gacgtcgatc cgacttccat ccgagaagag
     2041 cgtcctaccc gcgagggtga tctcgcctcc gtcaggcgtg aggatgccgg agatcaggtt
     2101 cagcaccgtg gacttgcccg ccccgttctc tccgatgagg acaagggtct ccccgcaccg
     2161 aaccgagagc gtgatatcga gaacgaaatc acgcagttgc cggacggcat ggacggagag
     2221 catcaggcgt tcctccgggt gatgatctgc accgcagcaa tcaccgcaaa cgatatcacg
     2281 acgaggacga ttgcaaggct gatcgcggcg gagaggtcgc cctgcatggt cgtatagatc
     2341 gcaagcggca tcgtctgggt ccggccctgg aagttcccgg cgaacatgat cgtggcgccg
     2401 aactccccga gcgcccgggc aaacgagagt atcgccccgg aaacgagccc accccacgca
     2461 agcgggatgg tgatccggag ggcgacggtc gtgggagatg caccgagggt ccgtgccgcg
     2521 tcctcgtaga gacggtcgat cgcctcgaaa ctcgccctcg cctgcctgat atagaacggt
     2581 gaggccacaa agacctgggc caggatcacc gcgaccgtgg tgaaggcaac ggagatgcca
     2641 aacccgtcga ggtattgccc gaccacgccc cggcggccga aagccatcag gagcgcaatg
     2701 cccgcaaccg ccggcggcag gacgataggg aggtcagtga gggtatcgac gatctcccgg
     2761 ccgcggtagt cacatcgggc gttcaccagg gcaagcggcg tcccgaagag gacgacgatc
     2821 gccgtgctca ccgtcgcggt agcaagggag agggccaggg cgtcgagcac caccggttcg
     2881 gcaagcgagc ggaagaacgc ctccggcgat attcgcagaa agagcgaggc gaccggcagc
     2941 gtgacgaaga ggaggaagag agcaagaagg acggtgagga ggagggtgag gggcgccctc
     3001 caaaaccgcg tccggccgcc gtgccggagc acttcacgtt catgccaaaa tcgggtcgaa
     3061 tccatattct gtcaggatgg cgctgccgtc cggactccgg acgaatgcta tgaacgattc
     3121 tgcttcagtt ttgtgtgagg actctgtaag gataccgagc ggatacgtgg ccatgacgtt
     3181 gtactccgtt gggatctcga tccgcgccac gttatccagg tcgcctttcg agacgtccga
     3241 cttgtagacg aacgccgcat ccgcctcgcc gagcgtcagt ttgggcacca cggagctcac
     3301 cgccgtctcc tcggagatga cgttccccat cacggcctcc cggtatgcct cgccgtacgc
     3361 gggatcggcg gccatcctgt cgagcacctt ccgggtataa tcgccgaagg gaacgtcttt
     3421 ggtgccgatg acgatctttg tgcccgactt attcaggtcg gcgagaccgg tgatccctgc
     3481 cgggttatcg accgggacga tcagggccat gctgttcccg aggaacggcg tcacggtacc
     3541 gttatccatg aacccggcgt cctgaagggc ctgcatgtgc tttgtgctcg cggagacgaa
     3601 gatatcgggg ttcgctccct gctcgatctg ggtgcggagg gcctgcgacc cgtcgaagac
     3661 caggtcgacc ctgacgtctg cgttctctgc ctcataggct ttgccgatat ccgtgaaggc
     3721 tcccgtgagc gacgcggccg taaagacggt cagggtcgtc tctcccggcg cctcggcggt
     3781 agtcgtgcac cctgcaacca ggagaccggc gatgatcaga agacttacgg caacaagcgt
     3841 tgccggggtt gacgttttca taacaacacc tttctcagag gattaaggct ttgggtatat
     3901 aatttagtgt actttgcgta catattaact aaaatttatt taccttcgcc gacgatatta
     3961 aaagggcgtg gtggtgccgt ggcgaggacg gagcccgggg tctcgggtca ggaagggtgg
     4021 gcctgccggc cggagatcgg tgccgggggt gttgggcagc ccgcaaggcc ggtagtgctc
     4081 cagcatatac ctgataagtg atctcagaga atccggccga att
//
LOCUS       DAJB01000220            4420 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_175196, whole genome shotgun sequence.
ACCESSION   DAJB01000220 DAJB01000000
VERSION     DAJB01000220.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_175196"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          899 a         1227 c         1254 g         1038 t
ORIGIN      
        1 aggtgtaaat tctatctatt caggttaaat gctcctgcct atcaaagtct ctccccgtcg
       61 ccgcgtgtgg gctgatgtgt ccttcagatt cgagaagcat gtctatgcag agtccacaga
      121 ggacgttgcc cgctacactc tcaagtacgg ccgcgtgaat gggatctatg aggacgaaga
      181 gatagccttt gatctcaagc gtacagtgtc ctcgatgctc cgggacggca tccccgccaa
      241 acaagtggtt gaggagacca gccagatcta tggcatcccc caagagtgcg tagaggaagt
      301 tatctcagcc tgtccccccc aataagccct ttccccaggt gcgcggcgtg aaagtgatcc
      361 tccccaaaac tatatctttc cagatacaga catcatcatg tatgccgacg aagacggtag
      421 tcgagcggaa gaaggcagcc ctcactcagg ctgaaatcca agccagtatc gatagactgg
      481 atgcggacat ggagagagcg gggaaaggtc ggaaatatac tcatgctgag ataaaaaaga
      541 aatatgggtt ataaatcgat gtgcgggtac atctggtgtg cctcctcgaa acccacgatc
      601 agatgaattt gagcaaatct gttctctttc gcccccatca ccttgtaaaa cacggtgtat
      661 ttcctctgaa catgtaaccg gtgagtgttc tccttccctt tcagtttctt cttgttgcat
      721 agtctgatcc ggtcgaagag gtgttcaccc aggcaaaact ctattttctg gatgattttt
      781 gctaaatact cgctatctct gtgctcaatt gcatcaagat gtttttctgc cgtctctgtg
      841 tatacgatct cgtatttcgt catgcgcctc cctcagacct cgacgccccg tgtacgcagc
      901 gcggcgatca caccctgttc tgcagcctca gaaatgggga tactctcttc cttcgcgagg
      961 aggtagacgt tctcgtggat acggacggag gtctgttgaa aaacctctcc ctttgcgttg
     1021 atgtgcttca tagtagatgc atagcaagta ttgccactta aaaagcaggt gtcaaacaaa
     1081 tcgcccctct ttaggcaccc tctctttatc gcggtctaag cagagcatta ggatacccga
     1141 aagtgtgccc acatgtgcgc acatgtccgg acaggagaaa agaatcatgg aaacgagaga
     1201 aaggaagaat ccgacgataa tccatcggat tagaaaaann aaagggtcca cactactgta
     1261 gcaccggata cttacgagta tctaaagaat acagccgtta acgtgggcca attagtagat
     1321 agagcggttt ctgaactccg aaaggtaacg cctcatggat tgatccttat ttcgcaaaag
     1381 aaggaagaat catgggcttg gggcgattcg aacgcccgga cttcgccgtg taagggcgac
     1441 gtcataacca gctagaccac aagccctgcg gatgccttat agtgttttgc accgtgacgg
     1501 ataaacgtgt tgcctgccgg aggtttcgga taggagcctt gcgaaaaaaa ttgggttaga
     1561 tgaatccgaa ggacttcagc gtcacatcgg ggttgacggc tcccacgtgg tagaccttgc
     1621 cctcggagag ctcgttgatg acgacctcgg ccggcgagaa gagcgaggtg tcgatctggt
     1681 agaagtcgaa gttggcttcc ttgaatatgt cgtagaacgg ttttccgtat cccttggact
     1741 tattgctcgg gagccggtcc aggtagtcct tgatctcggg ggcgttcatc gtcagcatga
     1801 tgctgccgtg gtagatcgtg gcgtcgttgg tgcagcccat cgccttcgtg ccgtccttct
     1861 tgacgggggc gatcggggcg tggccgatac cggcggtgat cttcctcgtg tcaaagccga
     1921 gctcgttcaa cttgtagacc gcggtctcga cacagcggcc ggcgacctgg accgagccga
     1981 cgatcgatgc cgtgggggcg acgaccgcgc aggtgttcgc cacatccacg ttgcaggcct
     2041 cggcgatatt ctccatcacg gcggcgttcg ggaggtggtc gctctcgagg cagatgaccg
     2101 cgtaatcgaa ctcgtcttcg tactcgatga cctcataggt gtgcttgggc tttaaggaga
     2161 gcgcccgtgc ggggccgctg cccatggcga agtacttgtt gacgttgacc gtccagcccg
     2221 ctttctgggc gccgaggcat gcaatggccg ggaagtcggt gctgacctcg acgaagggga
     2281 tcgggacgtt cttgatctgg cccatcgtta cgttgacctc gccaagcccg cccatgcaga
     2341 tctcggtgaa ccgtctcccg gtcaggtacc cgccggttgt gctgacgccg cagtctacga
     2401 cgcgtgcgcc gttgtcgagc tcgtgcggga cggcatggaa ttcttcggcg tattcaaaga
     2461 gttcctcaaa aatatccagt gccagttcgt ttacgctgag catgtggaaa acctcatcag
     2521 agtacaaacg gtggacgaac agataaggat tatcaaatcg tctgctccag tcccaggtgc
     2581 tccagcacgc gcccgggatc gtagcggaga ctgatcagcc gggtccgccc cttcccctgg
     2641 cggtaatgga ggtttaagag ccgcatcgcg tcaaatttct ggatcatctt atagtacttg
     2701 gtgtaactga ccgccacctg ttcttttacg aaccggtaga cgtcgccggc gttcatctcc
     2761 tggtcgtcgc gagccgccat ctcggcgatc ctgccgagca cctccttctc ctcggccttg
     2821 agcgcccgaa gcgagaacga aaggtgcaga taccgggaaa cctcgtacgc cttgcagaca
     2881 tcatcgcgct cgacaacacg gcgtgcctcc ttctcggcgt tcagggctgc ccgtttgaga
     2941 aggtcgatac cgacgcggag gtcgccgctc ttcatcgtct gctccaccac gaggtccagc
     3001 atctcgggcc ggaggacatt cgggtagagc ccctgcagga ctctttcttc aaggatgtcg
     3061 tggacctcct cgcctgagta gggggggaaa tagacctcgg tgggtcggaa gaccgatgcc
     3121 acccgggcgt cgacctcgct ctgcaggttg acggacatgt cgctgacgat ggcgatcacg
     3181 ccgatcctga cgcccggata ggcctcatga gagcgcagca gcgggtagag cacctggttg
     3241 atctcgttct cgtagaggag gtagtttgcg tcgtcgagcg ccaccagaag cacctgctcc
     3301 tcgcgctgca ggatccgggc tatggcatcg aagacctgct tgaacgatgt gcctgacgcc
     3361 ggcggcaggt gcccggtgat ccggcggtat atctgcgaga agatggcgaa tttggtgttg
     3421 tcgatctggc agttgatata gacggggacg agtttcttcg tggcctcttc gatctcggcg
     3481 aggactttcc tgacgcttgt cgtcttcccc gtccccggaa ggccccggca gatggtgttc
     3541 tggggcctca caccccggag gcccggccgg acctgaaaag cgagttcccg gatctgggca
     3601 tcacgatgat tgaactgctc agggacgtaa tcgatctcga agacttcagg gtctcgaaaa
     3661 agcgtctcct cccacatgag caggttcttc ttcattatac aatcctctgg tacagtagta
     3721 tttataacct ccatgttgtt atttcatcat cgcagagatc ctgaagggct taaggctgtt
     3781 ccatgcactt cttgcagagc gttttgctca tgaagagctg agagagcttt gcctgtgatt
     3841 tcgtcactcc tgctccgcac atctcgcaga catcctctcc cggagcggcg ggcggctttg
     3901 ctgcgactgg ttcctgcgcc ggtggtgcct gttccggggc tgcctccggt tctgctggaa
     3961 ccttctcctc cagttttacg ggtggttggg ggatccgggg tggctccggg tgttccggcg
     4021 ccggcggaat tgctgcctcg atgacgtcgg gcgaagggca ggctttctct tcctctccgt
     4081 gtgccggctc cgtctcgggg gtctccgggc cggcagcctt ctcctccggc gccggtcctg
     4141 cgggcggtgc tcgcggctcc gcaggtctga ccgcgagaac gcgccgccgg tatgcatcca
     4201 cacgctcggc ggttgcctga tcaccgcgcc cgagccgggc aagcatcccg tcaaggaacg
     4261 tgacaaggtc gtctacatcc tcatgggtct ctacatcccc ctcgacctcg aggcggaggt
     4321 tttcgtagtt ctcgaggttg atggtgatcc cgatggtgac ttccttcttg ctggacatat
     4381 attccattaa atattgtatt ccattatata caattccacc
//
LOCUS       DAJB01000221            2536 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_122689, whole genome shotgun sequence.
ACCESSION   DAJB01000221 DAJB01000000
VERSION     DAJB01000221.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_122689"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          581 a          660 c          757 g          538 t
ORIGIN      
        1 tctgacgccc cggatgacgc accccacatg cccggaggca aagggaaggt aagcattctg
       61 cgtacttaaa catgtgcctc tcctgcctcc gggctagaat gttatggagg taggttacat
      121 gaaaggaaaa actggaatgc gggcactttc cgtgctcctg gcggtaatgc tggtgagcgt
      181 ggttgctgtg cccgcaagcg catactacta ctgttgcgca gaatgggtga acgattatag
      241 caataacccc gattttggaa atttagataa gaatgacgaa aacgcacagg gattctacta
      301 ccaactcggt ggagactcat cgtggtgggg agactttatt tatggcaatg gccaggtcct
      361 ggaaagacat tggaaagatc ccagcaaacc cggtaatggt atagattata tttacaccga
      421 cgacatgcat tttgcatttt tctcgggtca cggctacaat gatggttcgg gatttgcatt
      481 tggcaccagc caggacgatt ttaaactaca ttacaacgac gccctctggg ggaatcacag
      541 atggactgga ttacactcga tgcctgcaga gttctgcgag aggataggta tacaaactgg
      601 tacagtgcat ttgacggcct gcactcgatg accggtttcc acacaggatg caccgatgtg
      661 gaggacaggg gatccaactt tgcacgccgg atggacggca cgtggaccac ccagccgatc
      721 atcactgcat ggttcattgc agcgaaggat acagagcctt cgaccacgta tgcggcagcc
      781 cttgcccgag aaggctgctg gtccgactat gtctacgggc acggaagcca gggaaccccc
      841 ggtgcgtcgt tcctgtacgg cacgtaccag tgttgaggtg atctagatga acatgaaagt
      901 aattgggata agcgtcgcta tgctggcgct tctgggaact atctgcgcga tcggcgtacc
      961 catgcttgcc gacgatcctc ttcgggatga tcgttccgac ctgacaggcg gtatgaaatc
     1021 gacgagtaat gcaaacctta cctataccgt ctctgtaccg ttcccatcga cgcccgaaca
     1081 ggtcgctcta tataaatcag taaaaccgga gatcacagcg gaactcgtct catcgatggc
     1141 ggagaagatg ggtctggagg gaacggtgcg tgaatcgtac gaccagatgc tgctctccga
     1201 tgaaccctac tcgctggagg tccatatgaa atcgggacgt gtcgccctga ttgacatccc
     1261 ccgctggatg aacccgaacg acaaagacct gccctcaaac ctaccttcgg atgagaaggc
     1321 gatcgagatc gcaacgagat atctggagga gacggggctg gcaccccctg atgcgaccct
     1381 gtgcggcgtc gatcatccac agatcgtcaa acagaatgag aatggcgagg ttatcggtat
     1441 cgcctttgaa gacgttcagg tctcgtactc ccgcacgatc gacggccggc cggttgtcgg
     1501 ttcgaaactg acggttgaag tcggcggtgg aggggatatc ctgaatgtat ataaactctg
     1561 gagagactat gtcccacagg aggagatacc aatcataacg ccggaggagg cgcttgaaga
     1621 gttgaaagtc gccggcgtgg cggagggcct gagcgcggat atcaccggga tcgaactcgg
     1681 ctattacgag gccccggcga cgagggagcc cacccacctt gtcccggtct acatcttcac
     1741 gggcaaggtg ctggacggga ccggggagga gacctcgttc gtccgctacg tccccgcgtc
     1801 tcccgagttc aggaaggagg tcccggcggt caagtgaccg ctcccggccc ccttcccttt
     1861 ttcggagcaa gcctatgaag aagatcctgt atcccgtcat cgcctgtgcc gccgcggctg
     1921 cactgatcct ggtcgccgca ctgatgctgc cggatatggt ctcggaagtc ccagagattc
     1981 cggagaatca gacggcccgg ctgacggacg ccctccggga gaatgtctcg ttcattgagg
     2041 tccgcgctcg cgggggcggg agcaccctcc tctacccgga agacccgggg tattttgccc
     2101 tagaaacgga gtgcctcgag caaattcggt gtatctctgg ccagtataag acagggttct
     2161 cgcgggagga actggacgcc atgaaacaga acggcaccta tgtcgcgatg tatttcccgg
     2221 tttcaacgac attcgtgacg ggttatatcg tcgatgggtt gccgaaggag atccttgcag
     2281 acgaagcggt gttctttctg gatctcgagg actggtcggg gaacatgatc gtcatgcggc
     2341 tggggaacga cggttgcggg gtctgggata cgtctcgcga tcgcggagaa ctccgggatc
     2401 tggttgatcc gatcttgttg gagttacgag cgagaatggg tgacgactga atactcggcc
     2461 ggatccgaag cgagcggaac ggtcattttc tggtcgcttg aggatgggta tgcgcaccat
     2521 ccgcagtaag aaactc
//
LOCUS       DAJB01000222           15322 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_38519, whole genome shotgun sequence.
ACCESSION   DAJB01000222 DAJB01000000
VERSION     DAJB01000222.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_38519"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2676 a         4778 c         4998 g         2866 t
ORIGIN      
        1 actccttgct acatctcttt gaagagcagg tgtcagcgcc agaggctgct gcctgtgcgg
       61 cgtgcagcgg ggtccccgac ctcggggtct gctgcatccc ggactctctc tacgcgttcg
      121 gcgatggatg ggcaatggtc gggtttggaa cagaaaagcc cggagatcat ctgcgggaga
      181 tcaccggacc ccgtgcggcg tggggtcgac gtcccggcca gtaggccgga gaggaggttc
      241 tggaggaggg cgccgggatc cctggtccgg tagcaacacc tcatcgcatc tgccgcgtac
      301 cggtcggcgg tgaactcctc gtcgcacatg cggcaataat atgtccggta ggcgaagaag
      361 acggtcagga ggaggagggc aatgaagacg aactttgtac cgccaggacc tccttcctgc
      421 agggacggat gcccaaagag catgaggcag aggagaaccg ggagggccgg gagggcagag
      481 aggaaggaag aaccttcttt agcatgcccc tcttcgtgga gcagcacgaa ccggagcgtg
      541 tcgtcgctgg cccgggagag gtttctctcg tagaacgaac gccggtagcg gatgcagttg
      601 agcgccgggc atccccgcag ggaggatgtg aaggtgcatc tcaccagcct cctcttgcga
      661 tcgatcagcc ccttctcctg gagttcccgg aatatccggt acaggcggga atcccggtct
      721 gctgcatcca ttctaattaa attataataa tcagcatata ttaaatgttg ctctgaatcg
      781 gaatgttgtc tattccgtaa tttgccgatt tccttcgtac taatcccggg acagcgggat
      841 ccccgcggca gggtaccggc cagacccgct gcagcacggc ccccctctgc cggcagctcc
      901 gaccgccata gggtgcgtcc cggatgcccg ggctcctgcc gatccgccgg ccccctgttc
      961 cggggaacgg gtccggacgg gcttctcgcc cctctccgga gcggccgggg tgagtcctgc
     1021 ctcctggcct ggtacggttc tccggtatcg gcccgggatc tgaaacattt gcaatgcccg
     1081 tctttttcac aatgtattaa taatagcagc caaaattgtg aatgaactga gtggaggtga
     1141 atttatagga ccggagggat cacatggccc gtgctcgtgc cggcacggca tgcgggtctc
     1201 cggtactctg agcaaatgtc cagcaaatac gtgagtcttc tgatactggc ccttgccgtc
     1261 ctcaccgggg ctgccgggtg cctctccacg gcatttgggg aggtgacgta tagcagcgac
     1321 gccctgcagg tccatgtgga gcacaccggt gagcccgttg agaacgccgt gctccaggtc
     1381 acgatcatgg aggtggtcgc gcttgaacag catgaagcct tctctgaagc gcggtatatc
     1441 aatctcgacc ggggagagaa cgaatacacg atcccggtcg gcctgcagcc tggctcgtac
     1501 aaggtctttc tgaccatatt cgtcggtaat gaacggagaa cgtctgtcat ccgggacctg
     1561 gaggtagcag cctgatcccg actggactcg cggcggcgcg ggggcttgaa ccggcggatg
     1621 ccgtcttctc cggggctgag gtcttcaacc cgttcgcgtg catctgggag cgcgtggact
     1681 ttgcgatgaa ggacggctat gtcgtcggcc tcggggagta tgaagcggag cgggagtacg
     1741 acctcgcggg ggcctgtgtg gtgcccgggc tgatcgacgc ccacgtccac atcgagagtt
     1801 ctctcctcgc gccggccgag tatgcccggc tcgtccttcg ccacggcacg acgacagtaa
     1861 ttgccgaccc ccacgagatc gccaacgtcc gcggggcgcc ggggatcgag tatatgctcg
     1921 ccgagagtat gcggacgccg ctcgatatcc tggtcatgct cccctcctgt gtgccggcaa
     1981 cgcccctcga cgcgggcggc gccgtgcttg ccgccaccga cctcgcccgg ttccgggggc
     2041 gggagggggt catcgggctc gccgaggtga tgaacgtccc cggcgtcctc gcaggcgacc
     2101 aggacctccg ggcgaagatg gacctcttcg aggtgatcga cggtcatgcg ccgttcctct
     2161 ccgggaagga cctgaacgcc tacatctacg ccggggtcca gagcgaccac gagtgcacaa
     2221 cgctcgcgga ggcacgggag aaacttctcc gggggatgta cgtcatgatc cgggaaggct
     2281 cgacggagca caacctcagg gacctcctgc cgctcgtgaa cgcctgcacg gcgccgcggt
     2341 gctgctttgc caccgacgac cgtcatgccg acatgcttgc cggaaagggg catatcgatg
     2401 actgcgtaag aattgcggtc gcgtccggca tcgaggtcga actggcgctc cgtatggcca
     2461 cgctctccgc cgccgaacgg ttcgggctcc atgatcgggg tgccctcgct cccgggaggc
     2521 tcgccgactt ctgcgtcgtc gacgacctcg accgtttccg cgtcctgcgg acgttcaagc
     2581 gcggcatcga ggtgatcgac cccggttacc gtccgccggc ctgcctcgcg gcccccctgc
     2641 acgcccgcgt gccggaaccc cgcgatatcc ttctttccgg ccggggagag gcgcgggtga
     2701 tcgggatcgt gcccggccag atcacgaccc gggacctgcg ctttccggtg gacgccgccg
     2761 ggatcccgga catcggccgc gacattctca aagcggtcgt caccgaccgt taccgggcga
     2821 gcggctccgg cgtcgggctc gtccacggct tcggcctgca ggaaggggcg ctcgccgggt
     2881 cggtctccca cgactcccac aacatcgtcg ccgtcggagt cagtgacgtc gatatcgtcc
     2941 gcgccgtcgg cgaggtggtc cggctcggag gagggcttgc ggtggtctct aatagcgacg
     3001 tgaccgcgct ccccctcgag tgcgccgggc tgatgtcggc gctcccccac gccgaggtcg
     3061 tgcggcggct cgcggctctc gaggagcatg cccggcggct cggggcagtc gcgaacccct
     3121 tcatgtacct ctcgttcctc gccctgaccg tcattcccga gatccgggtc acggagcggg
     3181 gggtctttga cgtccgggcc tttgcggacg tcccgctctt ctctgagtga ggggcggagg
     3241 gcatgcccct ctcccctcac ctgacagtgc tcaagttcct atcggaatgg tagtcactct
     3301 tcatcggccc ctccctacac cctcgctgct caaacttttg gccctgcagg aacatgttcc
     3361 tcgtaaagac ccggatcctc ctcaccagaa ccccggcacc gcctccggcg cgaggaccat
     3421 gagcaggagc aggataaccg gctggtgcag gaagtagatg acgagcgaat gccggcccag
     3481 gaaatcgagg ggccgggcga acgccggctc cgctaccggg agcgcaaagc cccgcctgcc
     3541 gtccgggtag aagagagacc cgcaggccat gccgacgagg acgaggccga accaggggag
     3601 gagcggcacg taatcgaggc tcgtgaatga ttcgggatgg atgccaagcc agaggagggg
     3661 ccacggccct gaaacggcgt tggtgacgta gcccgcgatg aggcaggcga tgctcgcgat
     3721 gatgagtttt tctgcgtcga accgcacgaa gagcggcgcg agtacgattg cgatgccgat
     3781 gaagtgcagg atgccaaaga cgatgtaggc cgacggcagg aagagccagg tgacggcggt
     3841 gatgacgagg ccgtagccga atatcgagag gccgcgccgg acgtacttga gcgggaggtc
     3901 acgcccggcg agccgttgcg aagcccgggc gtaactgatg gtgaaagaga ccccgaccag
     3961 gaagatgaac gtcgatgcgg tcaggtaggc gagcatccgg aggaacccgc cggagacgtt
     4021 cagcggcagg actccaaaga agttgaggtc gaaggcggag tgaaagagga tcatcgtcac
     4081 gacggcgatg ccccgggcga cgtcgatctc ccggtaacgc tcccccggca tggtttccgc
     4141 cgtcagaaaa accttctccg gaccggggcg ccgcaggcgg ggcaggtctg ggtatcggcg
     4201 atctccctgc tctgctcctc gttgggctcg ttgtagatcc gggagaagcc gcactgccgg
     4261 ctgcactcga tcacgacgaa cgggctacag atccgaccca cgccccctca ccccggcttc
     4321 ccgctgttcg tatatcatca gctcttctac actcagaagg aagggctatc tgataagcgt
     4381 ttacccgggn natgagcgac cacgagcata tcatagaagc cgccggcagg tgccgggtcg
     4441 tgatccggaa cggccgggtg gtcgaggtcg ggacgccgca gataaaagac tgcccgctgg
     4501 cacggcggtt cgcctgcccg atagaggaga tgacgcctga ggcgatacgc gagaacatcg
     4561 aggcgcgtat ccggtccttc gggatgtgca cgccggagcg ggaggtcctt gccgggccgg
     4621 acttcgtcat cttcggggct tcggaactcc tgagcagcgc catccggcga ggggagcttg
     4681 acgccgcggt gatcgcctcc gacggggccg ggacgctggt tgcgacgaac cctgccctca
     4741 tccagggaat cggcggtcgg atgtcggggc tcgtcaagac aagcccgatc ctggaggtga
     4801 tcgcacggat cgaggagaac gggggtgtgg tcctcgatcc ggagaccgcc gcaatcgacc
     4861 aggccgccgg tgtcgccctg gcgacaacgc ttggctacca gcggatcgcc gtgaccaccg
     4921 ccgttgccgc cgaggcggcg gctatccggg aacggttccc cgacaccgtg atcgtcgccg
     4981 tccacacgac cgggatctcg cgggaagacg cggcgctgat ggccggcgcg gccgacctgc
     5041 tcaccgcctg cgcttcaaag catatccggg aggaggcggc gaagaccgcg ctcctgcagg
     5101 cggggacgtc gatcccggtc ttcgcgatga cccgggcagg aaaaacaatc attcttggaa
     5161 agatcgggga gaccgaccag ccgatcatcg tccatggagc gcgcctcccg gtgccggggt
     5221 cgcagtcccc gtcgccgctc tgctgactcc ctacctgccg agaatcccca cgtggcccgg
     5281 gagttcggca aactcagcaa ggacgatatc tgcctcgaca ttcgctacgc tgctccggcg
     5341 ccagtcgccg taggcggcaa aggcggtcac catgccgagc agcctccccg gggcgatgtc
     5401 gcggaccagg ctgtcgccca ccatcatcgc cttcgccgga gccgtgtcga gctgtcggag
     5461 ggcatagagg agggagtcgg gctcaggttt tcgtcttccc gatatctccg gggtcaccac
     5521 gacctcgaag tgatcgatga gcccggtctt ctcaagccgc cggcgtgcct ggtacgactc
     5581 cgcgtcggtg gcgaccgcga gcccgatccc ggcttcttcc aggcaccgga gcgtctcttc
     5641 aacgcccgga taagccgtga ccccgtcgag tttcacgtcc tcgtaggtgc ggcagcaaac
     5701 ctcgaatgtc tccggctcgt agaccccgag atcgtttagg tagtcgcgta tgttcctgtg
     5761 gtcctcaaac ccgtggatgc cgcggagaaa ctgcgaaaaa agcgtctccg ggtctccggt
     5821 cccgaggtag tcgaccacgc accggcatgc ctcccgcttc gctcccacca ggtcaaagag
     5881 ggtgttgtcc atgtcgcaga ggattgcctc tatcttgtta tgccaagtgc tgagagtcgc
     5941 ctgcatatct ccagttccct tgtaaatgca cggtaggcca catcgtctcc gaagcgcacc
     6001 ttcagttcgt ccaggtcgtt gcccttcccg ccgtactcac cgaagatcgt gaaaccgttg
     6061 catccgacga acccgaagac ggcggcgagg tcaaagagca accgccgaaa cgccggcgcc
     6121 gtgcaggttg ttgcctcctg tctgtatggg cggtcaaagt agaagtactc gagcccctcg
     6181 gccatgcaga ggtctgccat gtagatgtcc gcggtgagaa gaaccgggag ggcataccgc
     6241 tcttcctcga acttccggag cgcccgcacg atgttacggt cgttctcctc ggaaagatgc
     6301 gtgtgggggc ccggcgccgc gatctccatc gcccggtcgc ggatagtccg atactctttc
     6361 agcgcgaggt acgccgcttt ccgggaccgt ttcatccgct tgttctcgag ttccccgatc
     6421 aactcccggt agccgggcgc gtgtgccgtc atctctccga tcagctttgc cgggtacttg
     6481 cggttgatgg cgtacgcgat ctcatcccgg acggtatcga cgatcaggta agaggagtga
     6541 ttgatctccg ggttgttcga tgcaaaaccg tggtagaaga ggttcgtatc caggccgaag
     6601 aagacggtct tcttgagctg cccgtaggat gtgagcatcg catcaaaagc ggcctggttc
     6661 acgtaacgcg cgatcccgct tgcaaacatg cattcctgga gatcggcgta ggtggggagt
     6721 tcgccggcca tcggtccgta cgcggcgagc cagccctgga aggtctcagg ggacgcgggc
     6781 agttcgatcc ggaagccggt cccttcggga cgtgcgacga ggaagtcctc ctcgtagagc
     6841 gggtacgaga cgcggatctc gtcgagactg ttgagaagaa cctgcaactc gtcctccctg
     6901 atgatcgcat cgttcatggc ctcacccccg catcctccat gatatcccgg atctcctgga
     6961 tctggtgcgg gatcatcatg tacggctttg ctacgtcgtc ggtactcttc atggcgaggt
     7021 agtagatgtg ccggagatca agtcccgcga gcgtgaccgc gataagcgcc ccggcgaccg
     7081 tgaccttcct cccggaggtg atatcgatcg ccacgcggaa cccctgcccg ataaggtctc
     7141 cggcaagcga gcggatcgtc tgccccgccc gtacaaagtc cgcttcgggc aggacctcca
     7201 cctggacctc cggaacgaac ccgtatccct cggagactat ccggattgcc tcggctgcgg
     7261 tcgatgcatc tcccgcgtag ggctcttcgg tgacgataga gacccgtttc gggcgcaacc
     7321 ccttctcccg caggactgca tgataggcgt tgacgagcgc ccatgttgag cgtccgagta
     7381 gtgttatgta ggcatcgccg gaggcgggtg tcacggggga gatcactgct caagactcac
     7441 cctgcttcta tataggcttt gtcggggccc ctctccctgg attggagttc acggccgggg
     7501 ttctgggcct gattctttgc tattttgcgc ttatatgctc catactcgct tcccctggaa
     7561 ttgtgtccgg agttctgcaa aattatgacc gcaattcact gccggggttc ttagaatacg
     7621 cctttagcgc taaagaaatc gcaaaatctc gcgaaatatt tggtaaatct tttatgatcg
     7681 gtatccctat agtggtatag aagagcgcga aattaccccg gagaatatga tccatggaga
     7741 taagaagagt gcgattacag acaccagccg acacctgggg gctggcggac ccggagcagc
     7801 agaggaaggc ccggcaggat gctatcgacg acggggatct gatcgagatc acccggatgg
     7861 gaagggacat gggcatcacc ctccccctcg cggtctcggc acgggccgcg cagagtatgg
     7921 tgccgtttac aaacatcccg caggataccg tcacggagaa cttatgggac acccttcatg
     7981 cgttcaggga taaagccctg acgacgaccg aggaggagtt cgagtttcag gtcagcctct
     8041 accagaacgg cctcgtcccc accctcacct tcaaagccac ggtctccccg ggagacgacg
     8101 gcgaaccggt catcaccatc atgatgccgg acgaggactg ggagacgatc gggtgcggcc
     8161 accaccagag cgcgggcgat gcgatgctca ctgtcgacga cgttgcctcg accctgaact
     8221 tcacccccgg ccggatccgg gagttcatca gggaagatcg gatcgccgcg gtcaagtgcg
     8281 gggggtcctg gcgaatcaag cgctcagagc tcaatcggat catgaacgaa ggtttctgac
     8341 cggttgttcc aggttggctg cggggccgta tcggccggca gacgacggag gttaatacgg
     8401 caggaggata aacactcctc cgacaacctc tgctcttcga agaagttctg cccgtgccga
     8461 taccgtccgt ctggatatac tgggtgatca gtctcaccat cgcgacctat gcaggtgccg
     8521 tagtcgtcag gcggtttccc gggtacgggt ttgccaccct gaccggcctg tatatcatct
     8581 accttggtgc ctcccagatc atcgcggccc ggcttattga gttcgatatc ggtatcgcca
     8641 cgtttattgc cctcctctcg cagatccttc tcgccgtcct catcgccatg acgaacaccc
     8701 tgaccccggc gccgatcttt gcgttcgaga cggcgtggca gagcatcttc acccaggggc
     8761 tgtggatcat cctcgcatcg tgggttgcgt tcctggtctg ccagaacctc gactgttacg
     8821 tcttcgaccg gctgaagcgt gcctaccccg accggatcgt cctccggagc gcggtctccg
     8881 atgtcgccga cctgacgctc gacagcgtca tcttcgtgag catcgctttc ttcagcgccg
     8941 ggattcccct gctccccctg atcctcgggc aggtcgtcag caagagcatc atcggtgtca
     9001 tcgatacacc ctggttcgtc tggtacaagc gctacctggg tgccgccggt ccgggggcgg
     9061 gcggggacgg acgccgccct cctgaaacag gtgttattta aaaattcttt tatcgatcag
     9121 gcacaggaaa caaacatatt taatttttag acgtagagtt ctcataatgt gcgccgaaga
     9181 cccaccagca ttcccaggca gctctttcgc tgaccctgca gccggaatgc acgatgtggc
     9241 gacagagttg ccggcatctg cgggtgggtg cgcgcccggg cctgatggtt gtcgtgcgcc
     9301 ttctccggcg tcatggaccc tggtgtgggc gatcttcggg ggcgggtgcc ttctcctcgt
     9361 tctgctcggt gcgggggtgc cggatctgcc atcgattccc acgatcgaag ccgtatgccc
     9421 catcgtgggc cttcccggac tttttgggcc gaacagattc tcgttcctca ggcactgggc
     9481 gaggaggggt actgaagaga gcggcactga ggacgggccg ggactctcct cgcccgatgc
     9541 gcccattgaa ccatccccgg ccggtgatgt gacgagcgtc cttgagagac ttgagaagat
     9601 cccggatacc ctccgttggt tcgacgatct cgactccccc gaccaggcga gtgcagccct
     9661 ccccgccggt gaacctgaag gagatcggat gctcccggag agcccggatg acgaactcat
     9721 gcgaatgctc gggttcgatg agggggatgt cgacgccacg tccgggcttc tcgaattcga
     9781 tgccttcctg caggaggacg agccggcggc tgcggccgct gcctgtgagg ttgccgtgga
     9841 ccaggaacgc ccggtggagt cgctcatcga cgacctcggc agtgatgatg ccggagtccg
     9901 cggacgtgcc atggatgcca tcgcagagca tggagccgat gccgtaactc ccctgatccg
     9961 ggctctcgct cttacaggtg acaggagacg gtggtgcgtt gccgaaaccc tcgtcttgat
    10021 cggtgaaggt tcaatcccgc cgctgatcgc tgcgctcggg gacagtgcga cgcaggccgg
    10081 ggcggctgcc acgctcgtgc gtaccggggg tcccgccgtg ctgcccctca tcacggcact
    10141 tgccggcgat gacagcgagg tgcagttcgg cgctctctac gcgctccggg agatcggcga
    10201 tgcggcggtc ccttccctcg tcggggccct tgacgcaccc gaagggagca tccgaagatc
    10261 tgctgcttct gttctccggg aactcgggtg gaaacctccc gatgacgccg gagcgatccg
    10321 ttatctcgtt gccggcgagg cgtggcttga tgtcgcggat tacggccagg ccgcagtcgc
    10381 tcccctcatc cgcatcctgg agtctccgga taaggagtca tggtggaatg ccgcccggac
    10441 ccttggggaa gtcggcgagg ccgcagtcgg ccccctgatc gatctcctgc accgggcagg
    10501 tgatgaggtc cggccgctcg tggccatggc actcacggag atcggtctcg ctgcggtgga
    10561 tccgctcatc gggatgcttt ccgacctctc tcttcggagc accgcggcga tggccctggt
    10621 taagatcggg gagccggccg tggaggcgtg cgtccgggcc ctggacgaca cggacgagga
    10681 ggcacaggaa gcgctccggg aaattctcgg tgcgctcggg gaagctgctg tcccgtccct
    10741 gatccaggcg ctgacatccg gccggtcccg gacccgtggc cacgttgccg atatcctgtg
    10801 cgggatgggc tgggaaccct ggagtgacgt cgagcgggca tggtacctga ttgcccggga
    10861 ggagtgggtg gaacttgccc tgatgggtaa accggcggtc gctcccctgg tccgggcact
    10921 caacggcgac gacgaccgca tacgcggcga ggctgcggcg acgctcggtg agatcggcga
    10981 tccgacggct gtagggccgc tcgtggatgc cctcgccgac gatgccgttg ccccggtggc
    11041 ggcggatgcg ctcgttgcca tcgggagacc ggccgtagcc cctgtcctcg aactgcttga
    11101 aggagagacc ggggaggccc gggagaacgc cgtcgaggtc ctgggcaggc tcggggcgcc
    11161 cgaggcggtc ccggtcatcg tcgaactcgt caggagcacc ggggaccgcc ttcaccggaa
    11221 ggccgtcgaa gccctggtcg gcatcggtga gcctgcaata ggcgccctca tccccctcct
    11281 cggtgaggnn cagcactccg gccactggcc gaagcactcg gcgatgagaa ctccctgacc
    11341 cgcatggggg ccgccacggt cctggagagg cttggctggg ttcccacggg ggcagaggag
    11401 caggccgtct acctgatcgc gttgcagcgg tggctggagg ttgtagacct cggagccccc
    11461 gccgccgatc ttctggcggc gtggtttggt gatccggagg tgcaggcggg ggtggcggag
    11521 tcgcttgcct gcctcggggc ccctgcggtt cccgccttga tccgcctgct tggtgaagag
    11581 gcccactcgg aacccgcggg cgataccctg gtgcggatcg gggaggcggc tgtggaaccc
    11641 ctgatccgtg cgctgagcga agagacgctc cgcccggcag ccgctgcggt tctggtccgg
    11701 atcgggaggc ctgccgccgg tgcgctcatt tcggccctcg ggcttcctga tgccgggcag
    11761 gctgcggcgg aggttctggc gacgatgggg gagccttccc tcgatgcact ggtcgaggcg
    11821 ctcgggaacg acgaccccgg gattcggcag cgagccgggg acgtgctggt cagccttggg
    11881 gatctggccg tcaccccgct catcggggca ctgggacacc cggacgatcg catccgcctg
    11941 gaggcgatcg atacgctcac ccggaccggc agaccggccg tcgctgatct tacggaggcg
    12001 cttttagacg agcgttaccg ggtacggctg ggtgcggcgg aggtcctcgg gagagtcgga
    12061 tgggcgccgg aaaccgaggg cgagactgtc cgctacctga ttgcaaagga gcagtgggtc
    12121 tcggttgccg agctcggggc cggtgctgtg gaaccgctga tcctgacgct caacgacccc
    12181 gacagtgcga ttcagatggg tgcggcacgg gcgctcggca tgatcggggc gcctgcagtc
    12241 acgaggctga ttggcgaact ccgcttcgag cagaacggcg ggcagagaaa ggcggtcgag
    12301 gcgctcaaga tgatcgggga gccggcggta gtaccgctca tcgatgctct ccaggaccgc
    12361 gactggcata tccgactggg agcggcacgg gcgctctcct gtatcgggga tcccgccgtc
    12421 gaaccgctgg tccgggctct ccgcgccgcc cccccggcgg tccagatggg agcggccgca
    12481 acgctcggca agatcgggaa cccgggcgcc atcgagccgc ttaccagtgc gctcctgcag
    12541 gaggactggc gcctggggcg tgtggtggtg cgggcgcttg gcatgatggg cgacgcggcg
    12601 gtcaggccgc tcctgcgcgt tctgcgggaa gggaacgata cggcacggaa gggtgcggtg
    12661 acggcccttg tgctgatcgg ggagccggcg gttcggcttc tccccgatgc gctcaccgac
    12721 gggcatttcc gcgtccgtgc cggggttgcg gacgccctcg accgtctggg ctggtcgccg
    12781 ggaccggggg aagagacggc tctctacctg atcgcgaagg agcgatgggg cgatctcacc
    12841 cggatgggcc cgcttgcggt cgagccgctg gtcgcggtct tgaacgacca cgacgacagc
    12901 atccggcggc gggcgacgaa ggtcctcggc gaggtgcgcg atccgcgcgc ggtcccggcg
    12961 ctgatggccc tcctccacga cgactactac agcatccggc gggaggcggc ggcggcgctc
    13021 gttgtcgttg gggtcccggc gatggatccg gtcgtatccg cactcggtga cccggacggc
    13081 gacgtccgga aacgtgcggc ggatgtgctg gccgtgatcg gggatgcgcg ggcgatcgag
    13141 cccttgaggg gcatcttcga cgacgaggac tggtatgccc ggagggccgc cgaggacgcg
    13201 gtggcgagga tcctggagcg ggtcgacggg gaaggtccgg ggaaggacgc ataggccgtc
    13261 tccaccggtt cacgttaacc cggcttatgc atccacgtcg attcccgaga tatacgccac
    13321 gccgagtttc gcccgccgga tccgggtgct gtccttgagg tgacgcatat cgacgaactc
    13381 gccttcgctc acgatccttt cgatctgccg gccgaggtcg agcgctgcat cccacggcac
    13441 cccccggaag atgacgccgt ggtcgtggag ctgcacccgc tctgctccag ccggtcccgg
    13501 ctcgacgagg gctgagagac gctcgccata ggcggagaga tcggcggcga gatcaatgcc
    13561 aacaccgaag ctgaccatcg tatcgccgcg gtacttcggc tcgtcgtcga gagcgaacca
    13621 ttcctgcaga ttctcttccg cgttgatagg aagaccgttc ttcggggagg atgcggcgat
    13681 tggtgacttt ttcaccccgg aactgcagac cccgtcgacg acggcccaca tcgccagagc
    13741 gacgatgccg gagaagtcgc gcctccgctc ccgggcaaag tcaaagaccg agcggtacag
    13801 ttcggagagc gcgatgccgc cgccgtcccc ggcgtcgacg gtgatgatct cgtgaaacgg
    13861 cccgtcaagc gcgacgttgt acccggagta gacgtagagc tccccggtat ctttcaccgg
    13921 cgtgaagaag tccggtgtgg cgttcccatc ggtgggcagc cagaccatcg agccgtgcag
    13981 cgtgatcatc tctccaagga gcgggagcgc gtccgccacg ctccctccga gcgcaccgag
    14041 cccgagcgag tactggaggg ccgagaacgg tattgaacgg atgtcttgct ccgtcaggcg
    14101 ggcatgtaac acctgatcca ggctgccggt gatccggagc gacgacctct ttgcagagac
    14161 cggctcgagg gtataggcaa cgccgttcct gacgaccgga gtccgggcaa gctcgttgat
    14221 gaccgaaagg ctgtcctgcg gtctcctgca ggcggagacg gcttcctcga cccctggata
    14281 gagggagaat atcggtgtca ccccggccat gtcgagaacg ttcttcgggt actcctggat
    14341 cccggcaagg gccaggatgc cgttgcgttc ttttaattgt tttttaacgg cgatgagaat
    14401 ccggatgcct gcgctgctca ggtaactcag ccctgcaagg tcgaagacga ccgatcggtc
    14461 atcgtcctgc agagacgcct gcacggcatc ggagagttct cccgcaccgt agccgtcaag
    14521 ccttccctta atgacgagcg tcagaatccc gtctcttctc tcggtctgca attccatatg
    14581 tatcctggta cagatatcgg gctgctgacg gttaattttt ctcaaatccg ctttgccacc
    14641 ggcttgtcgt gtgtctgact ttcaccgtcg gtattgtccc ggtcgcgatt gaaggtccac
    14701 actaacttcg taaccgttat cgctcctgtt atgtcatcct ctatgatcca ccgtgaaacc
    14761 caacagagcc ttaataatca tgatcactct ttccatcaca atttttctgc tatttccgca
    14821 ggctgtaccg cgaccggtca gcaatcgccg gaatcgcctc tccctactgc ggttgccgca
    14881 cctgcccctg actgcccggc gctcgtgttt acggatcgcg aatttgcttt cgagctccag
    14941 cggacacttg gcgcctcgta tgcgggagag gccgatatcg gcgagtgcct tcccactgcg
    15001 tcctggatct gggaagggga ctttgagagc tggtacagcg aatggaacaa gaccgcggac
    15061 aacttcaggg cagcgggcga cgagagcctc gcagccgggc acagcgttac cgcaatggaa
    15121 gcatactacc gggccgcaac gtattaccgc acggccgagt tcttcctgca tggtaatccc
    15181 gccgatcccc gtatcgtgga gacatgggga aagagccggg attcattccg cgatgcgctc
    15241 tcactcgatg tcgttcctta cgagattatc gccatcccgt acgagaatac cacgctgccg
    15301 ggttacttct atatggtcga cg
//
LOCUS       DAJB01000223           19073 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_93357, whole genome shotgun sequence.
ACCESSION   DAJB01000223 DAJB01000000
VERSION     DAJB01000223.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19073)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19073)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19073
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_93357"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3524 a         5851 c         6063 g         3635 t
ORIGIN      
        1 gcatcgatgt tcaggtacgg atcgatcttc accgccgtca cctgatagcc gcgatttttt
       61 aagaggcggc cgatagatgc ggtggtgatg ccctttccga gcccactcat gacgccgccg
      121 gttacaacga tatacttcaa actctcgttc ccctccggtt gctccagatt ctctatctta
      181 tgtatgtgat ctttggtatt gagaatgtat tgttccggca gtccccacga atcgctgccg
      241 gcgtaatggg cggtttttcc ggtgtccacc gcagtgctac ttcccgatga cggcaaacga
      301 tgtcgatgtc cgggtaagaa gcgtgtcgtc tgccgcgctc cggacctctc cgtctgcaaa
      361 cgccacccgg cgccctttgc ggatgaccct cccggttgcg gctatcgtcc cggtgttgac
      421 gccttttatg aagctggtat gctcatcgat cgtggcgatc ttctcgtctt ttgagagaag
      481 cgtatagagt gcaagcgcaa tcgcctcgtc tgcgagcgcc acgtagaccc ccccctggag
      541 ccacccggcg ccgttcaaca tatccggccg gacttccatg gtgaggctgg cgcacccgtc
      601 gccgaattca cccagttcta ttcccatcaa cttaaagaag ggatttgcgt ccctccctac
      661 ccggtttagc tcgtcaatgt agtccacgcg accgcgcccg tatgcgagag ggtttggagt
      721 ccgccgggat aagcgttgtg aataccgtgc ccgatacagt atttcatgcc tgttggcggc
      781 cgtggaatcg atcgaaggga tcgccacgct atccaggtcg ggccggtggc agggaccgcc
      841 tcgttattgt tccggcaaaa cctcatcctg agcagttcct ctcgaaaacg tggacaataa
      901 gggcgagggg gctctccgcc gccggttgag ccccggagat catataaaaa caaatatctc
      961 tttcgctctc caccagtaga tgtatggaag atgcatggca gcccgaagcg ctccggaaca
     1021 ggaggctcga gcaactccgg gctaccatgg acgattacat cgtcaagcat accgaccagg
     1081 tggaagggaa cctcccggtc ttcttcgggc acgttgccgc cacgctcgac aagacctttc
     1141 ccgacctgga cgacaggacc tacgatgact tcatcgatgc aaccaccttc acgctcgtca
     1201 acaccacgcg cgaggctcct acagtggagt ttctcgaaaa gaccctgcgg catgccatgg
     1261 ggaacaagcg gcggagaaag gggcgggcaa ccctcgatgt gattgtgggc ctcaaactga
     1321 tcgataccgg caactattat caggcgatcg attaccttgc gcgctacagg aactatgacg
     1381 gccgtatcaa cgcggcaatc gcctacagtt actacgccct gtccctggca acgaaccgga
     1441 agcagggcct tcgccccgac gatctggaac ttcatgcccg cgaggagatg ctgaacctct
     1501 cccggacccg cgccccgctc aaccgcctcg gggtcctcga tcgcaaggat acccggttaa
     1561 actcgatctt ctggttcatg ctggatctgg cgttcacctg gttcccgagt gaaccggagt
     1621 tctaccgcat cggtatctcc tggacaaagc acgaagggga cgtcgaacgc cgccagagcc
     1681 tccttgcccg cgctatagag cggttctcgg ataacaggtt cttcctgacg gaggcgttca
     1741 acacccgcgt agaactgcgc aacgggggcg atgccgccgc gattgtcaaa cagatgatgc
     1801 agcaatatcc cgacgaccac gagccactct acttcgggat gaaactcgcc attctcggag
     1861 gacaaccgag atcctacgcg agtttccgaa aactggcgct cttgaaagca tttccccggc
     1921 acctgcttct gctgctcgat gccgtgctcg aggtcatgtg caaccacaag accgagagtt
     1981 acctctgctt tgaggaggca aagaaggttt ccgggcggga ttactacatc acagcgctgg
     2041 aatacatcct ccgcgacttc atcgaaggag atgaagaccg ggcgaaacgg gcgagatctg
     2101 ctttcttcac gagcgtcgac cagtactgca cgcaggccct gaagattaag gaggagtgat
     2161 gagtggccgc gtatcccccg acccggtaat atgcaatccc gcgtctcatc tccggatagc
     2221 ccgttacccc ttcacctctg ctgaattcac ggtaattctc aggatgtctc gttaaaacga
     2281 ccaatctcga tgatggagca gaaaggcata acgatgaata tatctgtcgc gacagggaaa
     2341 acttcctacg aaaagcgctt ccgggcccca ataggcagat acaccgggga ggatcggtta
     2401 cgatgacggg aaacaatact acacgagaca ccgggagggg agcatccggg accgacggcg
     2461 tcgggggatc gccgtacctc agagaggatg tctgcctctc catcctccgt gaagcgccgg
     2521 acggactcgt tctggtggac ggcaagggcg aatgccgata cctgaacccg gcgtttaccc
     2581 ggatcaccgg gtatacccgg gaggatgtcc cgaccctgaa cacgtggttc gaacgggccc
     2641 acccaaaccc ggcataccgg cagaaggtgc agggcatggg gcaggaactg ctccagggcg
     2701 accgaaacac tctggtcgcc agcgtggtct gccgggatgg aaaggtgcgt gatctcgaga
     2761 tccggcgggc gccggtcgag ggaagctgca tcctgctcac ggtccgggac atcaccgagc
     2821 aggtcctgac cgaggagcat ctcaggcagg ctacatccga actgaccgcg gtgatcgagg
     2881 cttttcccga tctcttcctc aggttgaacg ccgacgggac gatcctcgac tccagagccg
     2941 gcaggctggc ggagacgtcg ctggtatccc gggcactgct cggccggcgg gtgcaggacc
     3001 tgctccccgc cggggtaggc gaggtgctcg gggatgcact gcagcaggcg gcccggacga
     3061 atgcgtctgt cgcacccctg gagttctgtc agatggaggc cggagagacc cggcattacg
     3121 aggcccgcgt tgtactgctg tgtgagatgc acctgatggt catcatcagg gagatcacgg
     3181 agcggaaaga ggcggagcag gaactgcatc ggcaccgcga gcacctcgag gaactggtcg
     3241 cggaacgaac cgcagaactc gagcatgcga accagcagct cgagcaactc ctctcctcca
     3301 tcgagatgac cgagaggaga gccgcggagg agtggctgga ttcatcgatc gagcaggaca
     3361 ccctgaatcc ggccgaaccc gggggggcaa ggctcaccac cgatgttgcc gggacggtcg
     3421 tcatcgtcag cccggaggcc gagcacctga ccggctatgc cggggaagaa ctggccggaa
     3481 gaccggtgtg gtctctcttt tcggacagca atgttcacga tatcctctcg gaggaggtgc
     3541 tggggcgggg caggtcgaca gcgtgctcgg agggggctgt actcgtgcga tgtgacggtt
     3601 cagtgcaggc ggtccagatc tccgccgacc cgatcatcga tgccatgggt ttcgtgatcg
     3661 gcatggcctg cacgattcgg aagatataag ggtgggcggt cagagccgaa agccgtagga
     3721 ctccgggtgc accggccccg ggtcctgggg tgccacggtg actcctacct ggtcggagta
     3781 gaacccgagc ccttcgaggg cggagaggag caccttctcc ggcgtgttat tgatctcgct
     3841 tacatgggcg agcatcgcca tgtggatctg atcggcaagc ctctgcaggc agcgggctgc
     3901 atcagggttc gagagatgcc cgcgttttga ccgtatccgg ctctttaaat acgcagggta
     3961 ggggccggtc tcaagcatat cgggacagtg gttgctctcc aggagaatgg cgtcgcagga
     4021 tgcgagccgg tcgaagatgg aggcggtgac ggtgccggta tccgtgcagc atccgatgcg
     4081 gaggtcccct tcagcgatgg agaacccgca gggttcccgg gcgtcgtggg aggtcacgaa
     4141 gggctcgacc gagaagtcgc ccacacagaa cggctctcca tacctgcacc gccggacctc
     4201 tgccggcgtc ggcccgcatt tctcgttgaa cgcctccagc gtgccgccgg tcgcgtagac
     4261 gggcacgccg agcctccttg ccaggacgtc gactccccgg atgtggtcga tatgctcgtg
     4321 ggtgaccagg atcccttcga tcagcgttgc atcggcgtcg gcgcatctga ggcggcgaag
     4381 ggtctcgcgg gcggagagcc ccgcgtcaat gagcagtgca ccccgctccc ctcgcagata
     4441 ggagcagttt cccttgctcc cgctggcgag gactgtcagt tccattgtag aaatggtggt
     4501 ctgtgcgggt atttaaggcc gcgtcgggga accgtgccaa acgagtccca tggttcccgg
     4561 caccgtactc agtcctgcat cgccctgacg acatggaccg actccggcgc aaacgagacc
     4621 acaacccggc tgccttcccg gatatcgaga ccgtccaccg ccttccagga gaggacggac
     4681 gagagcgcga acccgcagtc gaccgtcacc ttgctgaacg ggccgcgtgg cacgatggag
     4741 gctacggtgc cgggaagggt gttcccgttg agagcactcc tccctccggc cgccggggcg
     4801 atccgcaggt cctccgacct gatgcagagg cagacctctt ccccccgttt aaacgacgac
     4861 acggcccgga cctggacgcc gccgatatcg accaccgagg cgccgtctcc gccgtcgacg
     4921 acggtgccgt cgacgacgtt ctcggtgccg acgaaccggg cgacgtcccg gttcgccggt
     4981 aaggtgaaga cttcccgggg cgtccccacc tgtgcaaacc ccccgttcat cagcacgccg
     5041 atccggtggg cgagccgctg cccctggtac atgtcgtggg tggaaaagag gatcgtcgtg
     5101 ccgaggtcgc ggttgatccg gatgaccagg tcctcgatcg tctctaccga gaccgggtcg
     5161 aggtttgctg tcggttcgtc cattaagagg atctccggct cgatcaccat cgcccgtgca
     5221 agagccaccc gctgcatctc cccgcccgag agcgtccggg cccgtcgctc tccgtagccg
     5281 gcaagcccga ccacatcgag ggcgtcccgg gtcttcttac ggatcgcccc ctcgctcacg
     5341 cccctgaacc ggagcccgac ggcgacgttc tcggtgaccg tcgtgttgaa gacggcaggc
     5401 ttctggaaga ccatccccat catccggcgg atggagaggc ttttgtcctg ttccctgtgg
     5461 atgtcggtgc cgccgatccg gatcgctcct cccgtcgggg tatcgaggag gtcgatgagg
     5521 cgcagcaggg tcgacttccc ggccccgctc ggcccgatga tggcgaagat ctctctccgc
     5581 tcgacgcgca cggtgacgcc ggcaagcacc gccgcatcac cgaaccgttt tgagacgttt
     5641 gtaagttcaa tcatgcgatc accggtgctg caccgtggtt atggcgagat tgacgccaag
     5701 ggctatgatg aggaggatta tcccgagcgc aatcgactga gggatgtttc ccatcgaggt
     5761 ctcaagcgat atcgccgtcg tcagcacccg ggtcgaactc atgaaggacg acgccatgat
     5821 gttgccgccg acgaggatcg ccgctcctac ttcggatatt gcccgcccga acccgatcgc
     5881 gacggccgcg aggattgcaa accgggcctc cttcatgatg ttcgtgagaa actggagacg
     5941 cgttgccccg agcgagtgga gggtgtcctt gaggaccggg tcgactcccg agagcgcgga
     6001 tatggtcagg ccgatcatga tcggcaggat gagcactgtc tgcgcgatga tcatggcggt
     6061 cggggtgaag aggagccgca gaaagccgaa cgggccgaca cgggatatga gcaggaatat
     6121 caggagcccg acgacgacgg tggggagcgc atagagggtc tggatcaggc ctatgaggct
     6181 cttctttccg gggaacgtgc cgaagttgat cgcggcgccg agcggaacgg cgatcagggt
     6241 ggcgagcacg gttgccgtga atgagacgac gatgcttctt gcggcgatgg ccattacgtc
     6301 ggggtcaagc gtgatgatga gcctgatcgc ctccgtgaaa ccctcgatga tctcgtacat
     6361 gctctgcacc tgattgtggt ctgccggggg caaaaaaggg gtaggagtta aggccggatc
     6421 acagaacagg gtccgtcact tcagcctcgg tcacgccgat ctttgcccag tcgttctggg
     6481 cggcatagaa gagcggctgg ccgtacttgt cgacgccgaa ggaggcgatc tccttctgga
     6541 catcctcaga gatcatgaag ttgacccagt ctttcgcgac ggtgctgttg atatcggggt
     6601 acttccccgg gttgatctgc atggcgcagt agacgttgag caggatgtcg ccctcgtcca
     6661 cgagcgtcac gaggtcgagg tcgcccttgt aggcaaggta ggtcccgatg tcggagaggg
     6721 tgtaggcctg cttttcgttc gccatcgtca gggttgcacc catgcccgtc ccggcctcct
     6781 ggtaccagtc gccggagccc tgcacgtccg cggagtagtt gaacccggct gccttccata
     6841 tggccttctc cttggagtgc gtgcccgagg cgtcaccgcg cgagacgaag acaacatccg
     6901 atccgtcggc cattccagtc tcccggatgg tggtgaacgc cgcttcaggc tgcatgccct
     6961 tgatgcctgc cgggtcggac tcgggtccga cgacgacgaa gtagttgtag gcgaacacgc
     7021 gccggttgat tccatagccg tcggcgagga aggcatcttc acgggcacgg tcgtgcacca
     7081 tcaggacgtc cacgtcgccg ttctgtccgt actcgatcgc cttgccggtt ccggcggaga
     7141 cgatcagcac ctcggcgttg tactgctcct cgaagatcgg cgtgaggcgt tcgaggagtt
     7201 tggtgtcgta aaggcttgtc gtcgtagcga tacggagctg ctgcttatct gcgggggtcg
     7261 ggtcggtcgt cgtcccggta cacccgcaga tgagcaggag ggcgaccatg acggccgcaa
     7321 cggcaaaaag ggttcgtctt accatagtac tcagtatggt atctggacct gcacaattaa
     7381 atgtcgtggt tttacactga tggtaacttg attaacttga ccaggtatac ttgggggtag
     7441 gttacttgac ccgcaacgga acgccgggag ggcccggcgt gccgtgccca cctgccctca
     7501 ccccgtcacg atatcgtggt aggcgtcgcc gtccgcttcg acaaaaagga tcgccttccc
     7561 cccgctgaaa gcgggctttg gaaactttcg cagggtgaca gtgccgttgc tgactgccgc
     7621 cccgccccgg gtctccaccc ggtcgacgcc ggaggcgagg attgcggcga ttgtctggct
     7681 gccgtgaagg ctccgggtcg ttcctttctg aacgaaccgg ccgtcgtttc cggatgccgc
     7741 gaggccgatg ccggcaagcg caccgatgac gccgtcttcg gtgccgccga gcccttcaag
     7801 acggatccct gcctgccggg cgagtgagcg cgcctgctcc tgtgtcacga cagtcttctt
     7861 cgcagagaac ccgaagaggc agaggtcggt gtcgatgtcc tggtcggcgg cgacgcagat
     7921 ccccggatcg cttccctcga tgaagtcggc gagcatcagt tcctttgccg tcgcgaatac
     7981 gtcggccgcg gccctgttct ctgcttcccg gatgtggatg accgcagagc tgttgtgaga
     8041 ggtgtagggt atagcagggt ggacaaagag ctggtgccgg gtcacccccg tcaccgtgta
     8101 cgaccgggcg agttctgctg caatcgtgcg ggcgagccgt ccggttcccc gggactcgcg
     8161 ggtgtcggtg tcgtcgatgc caaggtagag ggtcatgtta tcgctcgtgc tctgtactga
     8221 tccgcccaca aaactaaata atacttctcc ggggtcaatc aaaagatgtt gactccgggg
     8281 ccgtcaggcc cggatgatcg tgccgacccg ttccccgttg atggcttttg taatgttccc
     8341 gggcacgtgg gcgttcacca ccttgatctc cgtgatgttg acggcgtcgc ggaggagctc
     8401 gaccgccatc ggttcgagca ccatatcctc catctccatc tccaggagct cgtcggccgt
     8461 gatctccggg atgaactcag cgtcggggtt cacaaacggg ttttccgtgt agaggccgtt
     8521 cacgttcttt ccgaggatgc agttcttccc gccgaccacc tcggccatca ggaaggcgcc
     8581 ggtatcggtc cggtgcggcg ggatgctccc gagtttcggc ggatgctcgt agagcccgta
     8641 gggcggggtg ccctggacga ccgggagcat cccgagggat atgagcgagg ggaggtttaa
     8701 gaggtcggtg gtgtggatcc ggacaccgtt gtacttcgag aagaggatgg acatcatgat
     8761 cgcgttctgc tcgctgatct tgcctgccag ttccgcaagc acccccgtcg gcataccgag
     8821 atcgatcccg acgtcgagga tgtgccgcac ccggacgccg ccgccggtgg cgaccaggat
     8881 cttgttgttc cgggagagtt ccccgatctc ttcgaccagc gggaagatca cctccttccc
     8941 gtagtcgatg accccgtggc cgccgatctt gacgacgttt aactccggca tgatcctgac
     9001 ctgccgaccg cttctggtct cgcggagcag gccccgccgg acaaggctct caccgcggaa
     9061 cctcgattcc atttcaaacc gttctgccat gttaaccctt gcttctatgt gaaggaaaac
     9121 attaagtaac tttgtgacat accagcaggt ataccacctg gtaggagatg aaaacaccat
     9181 gaagatctat gcacgagaac gccagaaagt cggcaccggc gtgaagcagc cccggttccg
     9241 cgtcgtcgcc gtcgtcgaag agaagagtga agagaagcac gtgaagatgg aggggaccca
     9301 cttccgcaaa gtagaactcg aacagatcgc aaaggatctc ggggcggagg tcgtctatct
     9361 cgaggagatg cccgaagaag agcggggcaa gaagaaagaa gaggcgtaac cagaccgacc
     9421 cccttttttg caccgggcgc gagtacttct acgatcggat gtccacacac gaacatgacg
     9481 atataatcga ggcggccgcg agaatcctcc tcgaagagga ccggtgcgta actatcggtt
     9541 tttacccggg cggcatcgcc atacgtttcc ccacgacccg gaaacttgcc gagcacctcg
     9601 ggatccccca ttactacgtc ctcccccggt tcggggagat ggagcgggac gggctgatcc
     9661 gccgggtgga gcgggtcggc atctccacca cggccgcggg gacagagcgg ctcctcgcgc
     9721 tcatggcgga gcggcacggc gagcgtgcgg aagaagtcct cggcgccggg gtcttttcgg
     9781 cactgcaggc ccgcaggagt caggcccctt ccggagcacc ggaagggtag acgtgggccg
     9841 ccgaaggccg gaaggcaagg tagacggcat cgcccctggc gacatcggct tcctcgaacc
     9901 cctgccgggt cagcgtcacc cggaatggga cactcgcgtc gacggagacc ctgaccagcc
     9961 ccgcgttctg ccgcaccgaa tcgacgcgac cgaaaaagac gttcttcccg ttctgcggca
    10021 gggctgcttt tgagaggagg atatcctccg gccggattgc aacggagacc gggccgggca
    10081 cacaactctc cacccgtatc cggatatccc ccaccctgac ggtcccgtcg ggcgacccct
    10141 ctccccggaa gaggttctcc accccgacaa agcctgcgac gaagtctgag ttcggcctcc
    10201 ggaagatctc ctccggcgtc cccacctgga ccacgtcgcc accgcgcatg atcgccaccc
    10261 ggtcggcgag atcgaagacc tcctcgaagt tatgggtgat atggatgatc gtcgtcccgg
    10321 tggcctcgtg gatccgcgcg agttccgccc gcaggctctc gcgcgtcttc acgtccagag
    10381 cagagagcgg ttcgtccagc agcagtgcgt cgggttccat caccagcgcc cgggcaatcg
    10441 ccgcccgctg ctgctcgccg ccggagagcg tgcccgggta gcggtggagc aggtggctga
    10501 tgtttaagag gtccgcgctc tcctgcacct tcccgcggat gaacgcggga tcggcgcggc
    10561 gtgacttcag tccgaagccg atgttcgcct cgacagtcag gtgcgggaag agcatgtagt
    10621 cctggtagac catgccgatg ttcctgtccc tggccggcac atccgtgatg tcccggccgt
    10681 taaggaggat cctgcctgca tcggggttgt agatcccggc gatcgtctcg aggaggatcg
    10741 tctttcccgc tccggtaggg ccgaggatga tgaagtactc cccgtctccg atcgagagcg
    10801 agacgtctgt tagcgaaaat tcgccgaggt tttttgacac agaatcgatg ataagcatgg
    10861 gtatacctcc tcaatagacg tgcgtggctc cgctgtagcg ctcgaagaca tagagcgaga
    10921 tgatcgagat tacaatgagg atcgtcgctg cggagatggc gtactcgagc tttccggtgg
    10981 acatgttcag gaagagcgag agcgggagcg tctcggtctt catccgcgtt gcgccggcaa
    11041 gcatcagcgc ggcgccgaac tcgccgatcc ccttcgacca ggtgatcacc gatccggcaa
    11101 ggaggccgtt tgaggacatg gggagcgtga cccgccagaa ggcctgggcg tcggtgcatc
    11161 cgagcgtctt tgccacgtgc tcgtagcggg ggttgatgct ctggaacgtc gagcgggtca
    11221 cccggagcat gtagggcagg ttcacgaaga actgggccac gatgatcccg agcggtgtaa
    11281 agacgaaggc gagccccatc gcatcaagcg acttgcctat cggggatacg ccgaagaaga
    11341 ggaggagccc cacgccggcc acgagcggcg ggagcgccat cgggatgtcc atcaccatgt
    11401 tcatcaggga tttgcccggg aacgtgtagc gggcgagcgc ataggcaacc ggcacggcga
    11461 ccagaatgca gaggagcgtg gagatgacgg acgtgatgat cgagagcctg atcgcaaact
    11521 ggatctcttc ggagaggagg cactcgataa ggacactcgc cggggagtgg gtgaccaccc
    11581 cgatgaggac cagcacgatg aaggcggtga tgaggagggc gaccgctatg gtcaccatct
    11641 ggaaacggga gacgttaccc ttgctcatga ggactcctca tgctcccgga ggtcctttgg
    11701 cgagaaactc cggaccttca cctcctcgat atgccgcgcg tcgacccagc cgctcctgac
    11761 ctcgtcgcgg tggtcgaact ggcggacgta gggtttgatg tcgagaagcg gcgtgccgtc
    11821 cagcacgtcg atgccgcgaa cccggacggt gctgtcctcg acggcaatga cttcgacgag
    11881 ggagatcccg atcgggttcg ggcggctgaa atggcgtatg gcaaagatcc cccgctcttt
    11941 gctgccgtca acgaacggac gctggagcag acggaatccg tctgcccggt ggaaatggta
    12001 gagaaggtgg atgtgagaga acccctcgat cccctgcaat ccttcggcat actcgggaaa
    12061 gacctcgatc gtcccctccg cttcggagaa gatgctctgg atgggcgtgg tgtcctggtc
    12121 ggtgaacggt gagtgaatgg ttccgatgat ctggtaggtg acttccatgc gacttctcct
    12181 tcgttggtcc cctcagggct tgacgcccgt gtaggtctcg ttcgggtagg tgatgaagcc
    12241 gtggctggta aagatcgcct ttccttcttc ggaggcgacg aacgcggtga actgctccgc
    12301 ctcatcgggg tgctcagagg acgtcagtac gccgatgggg acgatcttca tcaggttctc
    12361 cttgttcggg atgtagatga ggtccatctt ctccggcacg tagaggtcct cccagatgat
    12421 gccgacgtcg gcctggccca tggagaggta gaccagcagt tcgttcaccg tgccgctgcg
    12481 ggtcacgacg ttcttctcca catcactgag cagtttgttc ttctccagga gtttgtcggt
    12541 cagctgcccg atggcggtgg cggggttgtc gccgagagcc acccggacgc cgggcctcgc
    12601 caggtccgcg agggacgtga tgttggccgg gttgcccttc gggacggcga tgaccgggat
    12661 gtggtagacg acgagggact cattcccgac cagccccatg ccgcgggcag aatcgaagta
    12721 ggcggtcgcg cccggcatat agacgtcgcc cattcctgtc aggttcatct gggtgagcag
    12781 ggtgttcgat cccccgaaga cgtagttgat cgggatgccg gtcctgttcg tgaaggtcat
    12841 cgcgatctcg tccattggtt cccgcatgcc cgcgccgcag taaacgagca gctcgggttt
    12901 tgtcgtgtcg cccgccgccg gcgtatcggt ggtggttccg gtacatccgc atgcaaaagc
    12961 agcggctgcc atagcaagca gtgcaataat gagaagcagg tgtgctttca tgagaataat
    13021 tactcaagtg ggcaaaataa gcgtttacat tctcctcacc ggaaacgttg gtaaattaac
    13081 aaaacttatt ttggttaaaa ttaagtaaaa ggggtttatg gtgggggggg cacgcagggg
    13141 tacccttcca aaacccccaa aagggagagg agacataacg agagttgcac aatggcggag
    13201 agcaccggaa cggaggagag aggcatccgg atcaccctcg aggaggtcgc cggagccgtc
    13261 ggcgatttcg gcaccatctt ccccatcctg ctcggtgtcg ccatcgtctg ccccgacgtg
    13321 aacgtcagcc acttcttcct cttcctcgcc gcctggttca tcttcaccgg gctctactac
    13381 cgccttccca ttcctatcga gccgatgaag gcgatcgggg ccatcgtcat cgccgaaggg
    13441 ctctgtgccg gcgagatcgt ggcatcgggg atcgtcgtcg gcgcgctctt tctcctgctc
    13501 gggctcgtcg gcggcatggc ctggcttggg gaccggatcc ccaaaagcgt catccggggc
    13561 gtccaggcag ggctcgcgct cctccttctc cggacatcat ttaactacat catctccgac
    13621 gtcctggttg cggccatatc catcgggatc atcctcgcct tcttcgtcgc gtcgcagcgg
    13681 acccggatcc ccgacgtctc cgccctcctg gtcctcatca tcgggcttgc cgccggcatc
    13741 gccgtgcagg ggatgccgcc gttccacctg atgcccctcc cgaccctcgt catcccgatg
    13801 ccctcagact tcatagccgg cacctgggac ctcgtcctcc cgcagatacc gctcacgctc
    13861 gcgaatgcaa tcctcgccac atcgctcctc acctacgacc tcttcccgaa gaagggcgtg
    13921 gaccccgacc ggctctcccg gaccatcggc gccatgaacc tgatctcgac gccgctcggg
    13981 gggttcccca tgtgccacgg tgccgggggg cttgccgcca tgtaccggtt cggcgcgagg
    14041 accggcggag ccaacatcat cgctggcctc ttcatcctcg tatttgccgt cgccttcgcc
    14101 ccgccggagg tgctgaccct catccccttc ggggtcttcg gcgcgctcct ggtcttcgta
    14161 gcgctcgagc tcgggaagca cagcgtcaag acagaatcgt acctggtcac gggcgtcatc
    14221 gccgtcctca ccctcgcgat cggcctcacg ttcgcgttta ttgtcgggat ggtccttgct
    14281 tatgttctgg agcggcgggc aggacggcag gggagcgggc aacaacccgc atcctgaagg
    14341 cacccggacc cagggtttat ctggtactgc gggagatccg ggagagagac ttatggatac
    14401 cgtaaccgcg ctcggcctca tcgccggctc gccgaccacg ctctcgtttg caccgcaggt
    14461 cgcgagggcg tggcgcacgc ggtcgaccgc cgacctctcg cttgcgatgc tcgtcgtctt
    14521 ccttgccggc gtcctgctct ggctcgccta cggggtggtg aaaggagatg ttgccatcat
    14581 cgcggcgaat gccgtcacgg cggttctcat cggcctgatc ctctcgctga aaacaaagaa
    14641 cgggtgagac gcctcacccg cggatgacgt ggatcgccgt cgccttgacc gagagggtta
    14701 cgggggtccc cggaacaagg gagaggtcgc gcaccgaccg ccgcgtcagg accgcggtca
    14761 ggatgacgcc gcagtccacc ttcacctcgg cgacggggcc gttctccgcg accgagacga
    14821 ccttcccggt catcatgttc cgggcgctgg agcggcgttc gtccccgacg gcgaggacga
    14881 cgtcgtcggc ccggatgcag agggtgacct cctcgcccgc ggcggcctcc gtcaccgcct
    14941 cgaacgtccg ccccccggca tccaccgtcg cgtgcccgtc ctcgtttgcc gtcacggtcc
    15001 cgtccaggat gttctcgatc ccgacgaacg aggcgacctc gcggctctta ggcgtgttca
    15061 ggacaacttc ggcctcccct tcatccacga gggttccgtc gatgatcacc gccacccggg
    15121 tggcgaggag gtgcgcctcc cggcgcgagt ggctcacctg cacgatggtg aggccctcct
    15181 cccggtggag acgccggagg tcgtcgatga acttctcctg cgtgacgggg tcgagggccg
    15241 agagcggttc gtcgaggagg aggatatcgg gtttcaccgc gacggcgcgt gcgagagccg
    15301 cccgctgctg ctcccccccg gagagtgttc ccgggtagcg gtcggcgagg gggccgatac
    15361 cgaaccgttc gaggaggccg gcaatttcgt cccgggcttc ttccttccgc atccctcgca
    15421 cccggaggcc gtaggagacg ttgtcggcga ccgtcatgtt cgggaagagc gagtagtcct
    15481 ggtagacgag ggcgaccccc cgcttctccg gggggagagc ggtgatctct ttgtcccgca
    15541 ggagtatgcg gccccgctcc ggccggtgga ggccggcgat cgcctcaaga aggaccgtct
    15601 tccccgctcc cgacggtccg acgatgaagt agtagtcgcc cttaccgatg gcaaggctga
    15661 cgtctttcaa gcggaacgag ccgagcgcga gcgagacgcg gtcaaacgcg atcatcgtat
    15721 ctccccatga accgggtcat ccgtctgaga aggtagaaga ccccgaaact gatcaggatg
    15781 accgtaaacg cgatgctcct gctcgtctga atgccgtccg tcgtgaagga gtagtagatc
    15841 agcgtcgata tgataaacgg gtagtaagcg atcatgatga cgcctgcaaa ctccccgatc
    15901 gcccgccccc acgcgaggat ggccccgcta tacatgtgcc ggaggcttaa ggggagcgtg
    15961 accgtgcgga aggtggtgaa ggtgccggca cccagcgtgc gggcgacgtt ctccaggtgg
    16021 acgggcacct tctcaaaccc ctcccgcatg gtgttgatga agaacggcga ggcgacaaag
    16081 agcatcgcga cgaccgtgcc cgggtaggcg tcctcgaagg caagcccgat ctccccaaac
    16141 ggcgctccga gccatccccg tcgcatgaag aggaggtaga cgagcaggcc cgccacggtg
    16201 tgcggcagga tgagggggat gtcgacgatg ctctcgacga cccctttgaa gcgggaggga
    16261 cgggaccggg cgaggacgta ggcgagggga gtcccgaaga agacgaggag gaggacggcg
    16321 tttgcccctg cacagaacgt gaggagaatg gacccgataa cctccgacga ggttgccacc
    16381 tcgatgaggt gagccggatt ggcaagctcc gccgtcgcca tgttcgcgat cgcgagcacc
    16441 gtgatcccga cgagcagccc gccaaggatg cagaacccga tggtgcacca gtctacgtag
    16501 cgcttccggt tctctttcca ccgggtgagg gatgcctccc tgccgccccg gaggagaccg
    16561 cctgaaatga ctctggtctt catgtccgta tgtagttggt gctccggctt ttgggcggag
    16621 cgattgacat gaggacgatt gacggcggcc tgatgtaaga gtatcgtttt ttacgcggat
    16681 taacccgtgt ccgttaactt cccaccctct gcctgcggga tcaggtgcac cgccttcgcc
    16741 tggagggaga cccagacctc tatcccctca cgaagcccga gggtctctgc cctcctcgcg
    16801 gtcaccaggg cggtcaggtc gcacccgcag tgcaccgtca cgttcacgaa cggggccgtg
    16861 ggttcgatgg cggtaaccgt ggcgggaaag aggttctccg cctcctcgtg ccccggttcc
    16921 ctccggtgca gggagatgtc ttcgccccgg acgatcatct cgacttcgtc ggcggacggg
    16981 ggggttgtcg agaggatctg cttccccctc gcctcgacca cggtgagacc gtcgctccgg
    17041 gagacgaccc ggccggggac gatgttctgg acgcccacga atcgggcgat ccgtcggtct
    17101 ttgggctcgt ggaatatctc gcgggaagct cccgtctggg cgatcatgcc ctcgagcatc
    17161 accccgaccc ggtgggcgag acgctgcccc tgcgccagat cgtgggtgct gatcacgacg
    17221 gtcatgttgg cctcgcggtt cagccttgtt acgatcgcct cgatggtggc ggtcgacgtc
    17281 gggtccaggt tcgccgtcgg ctcgtccagg aagaggatct cggggtcggt cacgagcacc
    17341 ctcgagagcg ccacccgctg ctgttcgccg ccggagagat cgagtgcccg gctcttgatg
    17401 tagcgcgaga gcccgacggc ttcgagggca tccttcacct tccggtcgat gtcgtcgcgg
    17461 ggagcacggc ggtagcgcaa ccccatcgcg acgttgtcgt agaccgatgc gttgaagacg
    17521 atcggcctct gaaagagcat ccccatccgg cggcggaggt cgagccagct accgcgatcc
    17581 gcgatcgtgt cgatcccgaa gaccgagagt tccccctcgt tcgcgggctc gataaggtcg
    17641 aggatccgga gaagcgtgct cttcccggag ccgctcgggc cgatgagccc gaggatctcc
    17701 ccttcccgga ccgagaggtc gatgtcgtgc aggacttcaa ggttgccgta ggattttttg
    17761 atgtcgtgtg cctcgatgat cgccatggta tcaccgccgc tgcaccaggg aaaggacgat
    17821 attcaccccg agcgccaccc cgaggaggat gatcccgagt gcaatcgaga gggagtagtt
    17881 tgccatcgat gtgttgagcg cgatcgcggt ggtgaggacg cgggtggcgc ctctgatgtt
    17941 gccgccgacg atcatcaccg tcccgacctc ggcgatcgcc cgcccgaagc ccaggaggac
    18001 gccggccatg acggcgaacc gggcctccgc gatgatggtc atgaccgtct ggagcctgct
    18061 cgcgccgagc gcggtgatcg tgtagcgttt gtcccggtcg atcccggaga gcgccgcgac
    18121 cgtcagcccc atcaggagcg ggatgataag gatcgtctgg gcaacgatca tgccgccggg
    18181 ggtgtagagg aggcgaagga acccgaaggg gccgatgttc gagaggaaga ggaacacgag
    18241 caggcctacg atgaccgtgg ggagcgcgta cagtgtctgc agcgtggaga cgaccgcacg
    18301 cttcccccgg aactcgtaga agtagatgag cgccccgata gggagggcga tcagcgcggc
    18361 gaagaaggtg gcggtgaggg agatgtagag cgagcggatg gtgatctcca ccacctgcgg
    18421 gttgagggtg acgatgaggc ggatggcctc gatgaacccc tccacgatcg ggttcccgtt
    18481 caccatgtga actcaccttc cggactccct gccgcccttc caatcacctc gtccacatct
    18541 ccccgtgtcc tctcacgata cgcttttcac gttgttatcg gctccgcgca ggtgcagttg
    18601 aacgggggtt cggtgcactc gggtgcatag agcggcgtaa agagcggttt gccgaactcc
    18661 tcggtgccga agttcccgat cagctccttt gcttcatccg agatgagcca gttgatgaag
    18721 tccgtggctc cttgggcgtt cacgtccggg tgcatcgcgg ggttgacggc gattgcgctg
    18781 tagcggttca ggagttccgc accctccgcg acgatcggca ccaggtcgag ccggtccgca
    18841 aacgagagat aggtcccctc gtcggagagc gtgtatgcct gctgctcgtt tgcaaggatc
    18901 agggtgtcgc ccatgccctt gccggcctcg acgtaccagg ccccggcgtc gatgatctcg
    18961 gtgtcgtagt cgtagcccgc ctcctcccag agcaccttct cctggttatg ggtcccggag
    19021 ttgtcgcccc gggagacgaa ggcgacgccc gccgtctcgt tcgtcccggc ggc
//
LOCUS       DAJB01000224            3642 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_48871, whole genome shotgun sequence.
ACCESSION   DAJB01000224 DAJB01000000
VERSION     DAJB01000224.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_48871"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    3114..3126
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          634 a         1136 c         1247 g          610 t
ORIGIN      
        1 acattcatga aaccggaggt gctcgcggcg atggccccgt acttctcccg gtatttcggc
       61 aacccctcgt ccctctaccg gtttgccggc gagtcccggc agggcgtgga agaggcacgc
      121 gggcaggttg gagccgccct cggggcggag ccgaatgaga tcttctttac tgccggcggc
      181 agcgaagccg ataactgggc gatcaaggga gcggctctcg cgaaccggaa gctgggcgac
      241 catatcgtca cctccggaat cgagcaccat gccgtcctcc atacctgcgc gtggctggag
      301 aagcaggggt tctccgtcac ctacctgccc gtcgacgagt tcggccgggt ggacccgggc
      361 gacgtcgagg acgcgatcac cgataagacc accctgatct cggtgatgat ggcgaacaac
      421 gagatcggga cgatccagcc ggtcgcccag atcggccgga tcgcgcacga ccacggcgtc
      481 ctctttcata ccgacgcggt ccaggcggtc ggggcggtgc cgatcgacgt cgacgcaatg
      541 gggatcgacc tcctctcgct ctcggggcac aagttctatg ggcccaaggg gaccggagcg
      601 ctctacatcc ggcgggggac ccgggtcgag agccttatcc acggcggcgc acaggagcgg
      661 ggacggcgcg ccgggaccga gaacgtcccc gggatcgtcg ggctcggccg ggcgatcgaa
      721 ctcgcgaccg ccgatatcga ggggcacaac cgccggattg cggcgatgcg tgaccggctg
      781 gtccgggggg ttctcgggtc gatcccggat gcccgcttaa acggccaccc gatcgagcgg
      841 ctcgcaaaca acgcgaactt cagcttccgc tacgtcgagg gcgagtcgat cctcctgctg
      901 ctcgacgccc gcgggatctg cgcctccacg gggagcgcct gctcctcggc atcgtcggaa
      961 ccgtcgcacg tgctgctcgc gaccggcctc ccccacgagg aggcccacgg ctcgctccgg
     1021 ctgacgctcg gcgacgccaa cacggaggag gacgtcgact gcgttctgga cgtgctgccg
     1081 gaggtgatcg ggcggctgcg gcagatatca ccgctgacgc ctgccccctc ccggatgccg
     1141 ggggtcgact gatgtatgcc gagcgagtaa tggaagagtt cacaaacccc cagaacgtcg
     1201 gggaactcgt cgatgccgac ggtgtcggcg aggtcgggaa cccggcctgc ggggatatca
     1261 tgaggatcta cctcaaggtc gaggaggacc ggataaccga tgtccggttc cggaccttcg
     1321 ggtgcgctgc ggcgatagct tcgagttcca tggccacccg gatggtccgg ggtatgtctc
     1381 ttgctgaggc atgggccgtc tcgaacgccg acgtggtgga tgctctcggc ggcctcccgc
     1441 cggtgaaggt gcactgctcg gtcctcgccc gcgacgccat ccgggccgcg atcaacgact
     1501 accggacccg ccgggggctc gatccctggg agacggggcc ctgtgggtgc tgcggcgggg
     1561 ccgggtgcgg ttctcacccg gagaacggtt aaatacggac gctacgaagg taacaccatg
     1621 gttctcccgg aatatgttgt ggtcttctgc acggcccctg caggcgaggc ggaggcgatt
     1681 gcaaaagcgc tcgtcgatgc ccggctggcg gcctgcgtga acgtcaccgg cgtgcagtcc
     1741 tgttaccgct gggagggcac ggtcaagagc gagcccgaac ggctcctgat catcaagacg
     1801 cagcagagcc tcctcgaccc gctcatcgcg agggtaaagg gactccacag ttacgacctc
     1861 cccgagatca tcgccatacc catcgtcggc ggctataccc cgtacctgga ctggatacgg
     1921 gaggagacgg cctgatggag atcgtctgcc gcgccgggat ccaggtgacg ggcgacgccg
     1981 cgcgcaccgt ccacgacgac atcatcgtcg aggaccacgt caggctcttc ctgaacggcg
     2041 agtaccttac ggccctggtg gcaagccccg accgcctgga cgacctgggg gccgggttcg
     2101 tcgtcagcga ggggctgacc gacagggtcg actcggtgga agtctcgggg aaggacgtgt
     2161 atatcaccgc cccggtcggg agagacgtcc ggctcgagat ggagtcgtcc ggcggttacc
     2221 gcgtcctcgg cgagcccaga ggggttgcct cgtcgatcac cgtcacggcg gacggcatcc
     2281 gtgcggtcac ggcggcgatc gagtccgata cctggcggag gaccggcgcc gtccactgtt
     2341 cggtcctctt ctgcaacggg gaactcatca cccgggcctg cgatgtgggg aggcacaaca
     2401 ccgtcgacaa ggtcgtgggc tacgcggccc tccgggggct cgaccggtcg acctgcatcc
     2461 tcgggtgcac cgggaggcag ccggcgggga tggtggcgaa ggcggcgaac gcaggcatcc
     2521 cgatcgtcgt ctcccgcgcc gcctcgaccg accgggggat cctcacggcg gagcaggcgg
     2581 ggatcaccct ggtcagcttc tcgcgggggg agcggttcac catctatacg catcccgagc
     2641 gggtgcctga agtccttgaa caactggaga agacagaacg atgacggaga acgacgaaaa
     2701 aacagcactg atcctgctcg ggtgcccgca ggtgcccgtc cagacgagca tggcgctcta
     2761 cctggtgcac ggcttgaagc agcagggcat ccggccggtg attgccggga cgacggcggc
     2821 gcggaaactg atggaggtgg ccgacccggg ccggtactac ctggacgaga tgaccgatct
     2881 cgacgttgcg atcgatgaga tcacggggaa gcagcgggac ttcgacctct gcttcgtctt
     2941 catccataac gactccggga ttgcctatgc gggaacgatg gcctacatct cgaaggcacg
     3001 gctgtatgcg ctcatcttcg gggagcacgc ggaagaactc gccggcgaga tcgagttccc
     3061 ctgcgaggtc gtcgcggtaa aggccgtgca caaccccatg ccgctgaagc ggannnnnnn
     3121 nnnnnnacga ggtcgagccg ggctacaaga tccatgatgt ccacatcatc ggtgtgccgc
     3181 cgctctatgt cggcatcgag ggcgaggacc tgatcttccc ctacaccaag ccctgccacg
     3241 ggacgttcgt ggtgaaggtc cccggcggcg aggagatcgc gcggctgcgg gcgaggaagg
     3301 ggaagtagcc ctttctcatc tatcgggcct ctaaaatgtg gcgggtgcgt tcaggattaa
     3361 ccctctgcat ggctgttttc tgtgaccnnc acgtatccca gttttgattc gaccgtccgg
     3421 tcgtgtgcgg ggttgatgta gtagaaccgg acgaacccgg accccttctt tgccgcgtcg
     3481 atgaactgcc gggtgtaggg gttgccgaac gcgtcgagtt cttcagcccg gttcacgccg
     3541 atcttttcgg ggtcgaacgg gtgggcgagg gtggtgttgt tgaagtcgta ggcgtagatg
     3601 tagagttcgc cccggacaaa cgttccgttc gggtcggaaa ac
//
LOCUS       DAJB01000225           21933 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_25081, whole genome shotgun sequence.
ACCESSION   DAJB01000225 DAJB01000000
VERSION     DAJB01000225.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21933)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21933)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21933
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_25081"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    10920..11000
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11676..11821
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15961..15990
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4228 a         6822 c         6798 g         3828 t
ORIGIN      
        1 cctaatcgct acgggaggtt aggctgataa tatgtattcc gcgagaggct ccgatgctgt
       61 cccgccctat agcggcacgg tgaaggccgc gaacgccgcg agtgccgcaa acgcgagaaa
      121 tgcgaacatc ccgaccgtcc agacaaccat gaagaggaga accgtcacca gacgggcctc
      181 ccggaacggg acggttcccg cgttcatccg ccgtgccgtg gcccaggcat cgtaaatccc
      241 gaagagccag acggccacgc cggggatgag catcacgaga agccctgcga gcaccccgaa
      301 gagtaccaga acgcccttgc ccgtttcgcc gttgtagacc tgcccgagcc ccggaaagag
      361 gagggagagc cccgccgcaa acgccgggtt cttctccccc aaaaccgggg tatccgccct
      421 catccccgga acgggagttc cagggaatcc aatcggttct ctcatgattt ccgtacgtgt
      481 actgctctcc gggggaggaa agaggttccg gcccatccgc atcacaactc cgtcaggctc
      541 ataacctcag aagttatccc ttgttgagga tacccacatg acacgaagag acgtaacccc
      601 cgatgaagca aaggcgatgg gcgagcagct cggcatcacc tggaaggagt tcgatgtcga
      661 gcagttccgg cgcggcatgg tcgtggaact cgaacacggc ctccatgacc cggcgacgaa
      721 cgtcaccgac gacgacctct tcctgacggc gaagatagcg cttgcgcact taaacgagtt
      781 cccggactac tatgaccggc tcgaggagat ggaggaagag gcggaggcct actgggcgga
      841 gcgaaagacg cagggctgag gaggtcacca ccacgacagc cctgacgtga gggtcgatac
      901 cgccgcaggg gcgaccggca ggaagagaag gacagccgca acgctgctct cgcggtacgg
      961 caccgtgccg ccgttcatcc tccgtgcgac ggcgtaggca ccgtagagcc agatggccgc
     1021 cccggcggga gcgaaggaga ggcccccgaa cagcgtccca acgaggaaca gcacgccccg
     1081 caagaactgc ccgttgtatg cctgcccgag cccgacgaag acgagcgaga ggagcgcgga
     1141 gaatccaggt tcttttgatt caagggtaac cgaaggggta attctgcagg tcataggcac
     1201 cggcacctga aagggtaccg cgaggagaaa tgctttgtgg gcgatggggg gcctgccgcg
     1261 aggccgggcc cggggagggc ccggtcgggg agcgggggag gtgcgcaaca actattgccc
     1321 gtccgttgca accatatcgt atggaacggc gctcggaaag agagtggatc aagatacggc
     1381 aacggcgagt catcgatgaa gccgagtact acgccgatcg actctacgag acccagcggg
     1441 ccgcaaccac ctacgcccgg cagctgcagg gtcggggaga tgaggtcctc gtccttccga
     1501 ccgtccatgc gggcgggcac cggggttacg ctgtctttgt cagaagataa gagtgcgttt
     1561 ttgccgcgca ggcagccgcc cggctgtctg tccggtggag ggggtaagtc agcacaggcg
     1621 ggtgccttcg gccggggcaa taacagagtg gggggcgaac cccatccaca ttccccatta
     1681 ctccgtcaca tggggggatg atatattccc cgtacgactg aagttcgagg gttgaaccag
     1741 ctggttcaac gcacccggca ctcccggatc gccggctctg tgcattgctt ccgccagtca
     1801 tcgtggagga ccccccagac gtcgacatcc tcataaccat cccacttccg ggcatgcccc
     1861 cggaggcgcg cctcatgcat catcccacac ctccccatca cccggcccga cgccggattc
     1921 cgggagaagt gcatggcgta gacccggtgc agctcgagga cggagaaccc gtactcgatc
     1981 atcgcccggg cggcctcggt ggcatacccc cgaccccagc agggcttcgc gacccagtag
     2041 ccgagttccg cccgaccgtg acggcggttg acctccgcga gcccgaccgc ccccacgagc
     2101 tccccgtccg gggccagggt gacggcgtag atggcgtgca tccccttctc aaacccggga
     2161 cggtgggtgg caatccagtc ctccgccgct ccgtcggggt aggggtaggg gatctcgagc
     2221 gtgcgggacg cgatctcata atcaccggcg agccgttgaa ccgcaggcgc atccgcgagg
     2281 gtgaacgggc gcaggaggag gcgttttgtt acgaggagag gctgtccgct catacgggtg
     2341 ctctgtgcag cggcgaggga gaatacgccc cgggacggct gaccggctcc cggaaaaccc
     2401 cccagacgtc gacatcctca tagacacccc acttccggac atgttcccgg aaacggcctt
     2461 cgtggagcat cccgcacttc tccatcaccc ggcctgaggc cgggttccgg gagaagtgca
     2521 tggcatggac ccggtgcagc ccgagagtcg agaacccgta ctcgatcacc gcgcgaaccg
     2581 cctcggtggc atacccccgg ccccagcagc ggctcgcgac ccagaacccg agctcggcac
     2641 gggagtgctc ggtgtcgatc cgcaggcgaa cgccgccgat gaggctcccg tcctcggcga
     2701 gcgtgacggc gaaggccgcg gcgtcgccgc gctcggtgcc ctcgcggagg gaggcgatcc
     2761 agagctccgc gagaccgtcc ggatacgggt aggggaggag ggtcgtcgcg gcaacggcgt
     2821 agtctccgca gagtcgctga accagtgggg cgtccggaag gtcgaacggt cgcaggagca
     2881 ggcggccggt tgcgaggata ggctgattct ccatggatat atgctcatag caccgaccgg
     2941 atataaatcc tgccgcgccg ccgcccgggc cgtgggagag gacggcacga agagatccgg
     3001 cagatgctcc cctgccatat cacctgcatg aaatcacccc tgtttatctc ttgggttccc
     3061 atttaccggg attaaacgag ttaatagaca ttttgggcac tcaagaatcc ccgtttgaga
     3121 taagagccca taatatcacc gctatttgca aatcagcaac ctttattact ttttgttctg
     3181 tattccatca tacatgatca aagtcgcgat caacgggtac ggcaccatcg gcaaacgagt
     3241 cgccgatgcg gtcgccgcac aacccgatat ggaagtgatc ggggtctcaa agaccagcgt
     3301 ctctgccgag gcattcgtcg caaaagagcg cgggtatccc ctctacatcg cggacctcgc
     3361 gaagaagccg gcgttcgaga aggccggtat cgaggtcgca ggcgacgtcg ctgcgatgct
     3421 gaaagccgcc gacatcgtcg tggacgccac ccccggcggt gtcggggaga agaacaaacc
     3481 aacctacgaa aaactcggga tcaaggcaat ctggcagggc ggcgaggcac acgaggtcgc
     3541 cgggttctcc ttcaacgccc acgcgaacta caacgaggct gcaggaaaac agtttgcccg
     3601 ggtcgtctcg tgcaatacca ccgggctcgt ccggctcatc aacgccatgg accaggcctt
     3661 cggcgtcgag cgggtccggg cggtgatggt ccggcgcgga gcggacccga gcgacgtgaa
     3721 aaggggaccg atcgacgcca tcgtcctcaa cccggccacc atcccgagcc accacggccc
     3781 cgacgtccag accgtcctcc cgcacataaa catcgtcacc ctcgcgatga tcgtcccgac
     3841 gacgtttatg cacatgcact cgatccagat ggacctgaaa cgcgagacca cccgggacga
     3901 ggtcctgaag gtctttgagg accacagccg catcggcctc gtccgcaagg ccaccggcat
     3961 caagagcaac gccgagctcc gggagtacac ccaggatctc ggccggccga ggacggacct
     4021 ctgggagaac ggcgtattcg aggagtcggt ctcggtgctg aacgggaagg agttctactg
     4081 cttccaggcc atccaccagg aagccgacgt catccccgag aacatcgact gcatccgcgc
     4141 cctgatggga acggtgaagg atccggaaga gtccatccgg atgaccaaca aagcgctcgg
     4201 cctcgtcgcc atcgggtgaa cggcggagca ggggaggaag aagcattaac tccccggcaa
     4261 acccattcct tttaatggat ccgtacgaac tggtgacccg aaataccgta gaggtcgtca
     4321 ccgacgagga actgcgcgcg ctcatcgacc gtccggtcaa gcgggtctac accggctacg
     4381 agccgagcgg ggagatccat ctcggccaca tggtgacggt gaacaaactg atggacctgc
     4441 agcaggcagg gttcgaggtg accgtgctca tcgccgatct tcatgcattc ttaaaccgca
     4501 aagggaccat ggaggagatc cgggagaccg ccgagtacaa ccgccgctgc ttcgaggggc
     4561 tcggcctcgt cggcgcaaac tacgtcctcg gctcggacgt gcagctcacg ccggagtacg
     4621 aacttgccgt gctgacactc tcccaggcga tcaccctcaa ccgggcgaag aggagcatgg
     4681 acgaggtcgg gcggcagatg gagaacccca cggtctccca gatggtctac ccgatcatgc
     4741 agatggtcga tatcatgacc ctcaaggtgg acgccgccgt cggcgggatc gaccagcgaa
     4801 agattcacat gctcgcccgg gaacacctcc cctcaatcgg gtatccggca ccggtctgca
     4861 tccacacgcc gatcatcaac ggcctcgacg ggaagaagat gtcgtcgtcg gccggaaacg
     4921 tcatatcggt cgccgattcc gaggagggta tcaggcagaa gatgaagaag gcgttctgtc
     4981 cgccggaggt cgaaaacaac ccggtgctcc agatcctgcg ctaccatgtc ttcccccggg
     5041 ccggggcggt caccatccgc cggcctgcga agttcggcgg ggaccgggag tttaccgtct
     5101 acgaggacct cgagcgggcc tacgccgccg gcgaggttca cccgatggac ctcaaagcgg
     5161 cgaccgccga ccacctcatc gatatcctcg cccccgtaca cgattacatc tgcagcaggt
     5221 gacctgaccg tgacccgcga tgaagagcat gatcagttcg accgcaagat cgaggcgatg
     5281 ggcgtcagga taaaagacgc cgacaccgtg aaggtacggg acgatgtctt cgacgaggtc
     5341 accctcatgg ccctctacaa gctcgtccag aagaaactga tctcggtcat cggcgggcct
     5401 atcagcacgg gcaaagaggc aaacgtcttc tacggcgagc atgacgggag ggggctcgcc
     5461 atcaagatct accgcatcca gaccgcgaac ttcaaggcga tgaccgagta catggcagga
     5521 gaccgccggt tctcgagcgt ccgggggacg cgcaagggcc tcatctttgc ctggacgaag
     5581 aaggagttca gcaacctggc ccgggcgcac gacgtgggga tcccggtccc cgagccgctg
     5641 gtcttcgacc ggaacatcct cttgatggag ttcctcgggc gtgacgaggt gccgtatccc
     5701 cagctccgta acgccgaggt cgaggactac ggggcggcat accagcagat cctcggttac
     5761 gtgaagcagc tctaccagga ggcgcgcctg atccatgccg acctctccga atacaatatc
     5821 ctctaccacg agaaaccata cctgatcgat atggggcagg cagtcaccct ggatcaccca
     5881 cgggcgcccg tgtttctgat ccgggatata aagaacctca acagatattt ctcccgctac
     5941 tgcgatgtga cggatgagga agaggtcctc agagcgatcg tcggcaccgg acgccgggag
     6001 ccctgaacac cggggagaag ggatgcaatc atgaccacac aggagataaa agtttcaaca
     6061 gccagaatcg gtgtactcat cggcaagagc gggtccacaa agcgcgagat cgaggagaag
     6121 accgggataa ccctctggat agacagtgag gaggggatcg tgacgatcga ggcggaggat
     6181 cccatcgccg ccatgacggc gacgaacgtc atccaggcga tcaaccgggg gttctcgccg
     6241 gagcgtgcgt tccgccttct tgaggacgag gacatgatgc ttgatattat caacctcgca
     6301 gacctctcgg acacgacccg gcagctcgag cggctccggg gccggatcat cggcaaggcg
     6361 gggacgtccc gctcgcagat cgaggatatg accaacacgg agatctcagt ccacggaaag
     6421 accgtcgcga tcatcggtct cccggagcag gccgagaccg cgaggaaagc ggtggagatg
     6481 ctgatccagg gcgtgccgca cgagcatgtc tacgggtacc tcgaccggaa gaagaaggaa
     6541 gccaaacagt cgatgctgga gtactactcg tgatgcgacc ggggcatatc ggccccgtcc
     6601 atgaccatga caatacgtgg aatatgcaat ttgcagtgga aatgaagggg tatacatgga
     6661 gataaccggc ctgccgatcc cgccggatct cgcggccggc tacgctcggt gcgggatcac
     6721 ggaactctac ccgccgcagg ccgcctgcgt cgaagcagga cttttttcgg ggaagaatct
     6781 cctgatcgtc atcccgaccg cgagcggcaa gaccctggtc gccgagatgg cgatgcacca
     6841 ccaggtgggg aggggcggca agtgcctcta catcgtcccg ctccgggcgc tcgcaagcga
     6901 gaagttcgag gagttctcgg agaagggtct ccgggtcgga atcgccaccg gcgacttcga
     6961 ccggcgggac gagtacctgg ggagaaacga catcatcgtg gcgacgagcg agaaggtgga
     7021 ctcgctcctg cggaaccgga cgccatggct cgccgagatc actctgctgg tcctcgacga
     7081 ggcccacctg atcgacgacc cctcccgggg agcgacgctc gagatggtga tagcaaagct
     7141 ccggcacaaa aacccggata tgcagatcat cggcctctca gccaccatcg gaaaccctca
     7201 ggacctggcc gggtggctgg atgcggagct cgtcgagagc gactggcggc cggttgacct
     7261 ccgcgaaggg gtcttctttg aagacggcat ccggttcgcc gacagcaccc gggaagtcag
     7321 tcgccggagc aagtacgagg acctggacct cgtcctcgac acggtcgcag agggggggca
     7381 gtgcctggtc ttcgtcagtt cccggaagaa cgccgaggcg tttgcaaaac gcgccgcttc
     7441 agggctgaaa ctggcaaacc caaccctcgc cggctatgcc gataagctgc gctcgaacgc
     7501 cgcgaccgat atggggagga cccttgccgc ctgcgtcgcc cagggggcgg cgttccacca
     7561 cgcggggctc gcgcgggagg agcgccggat cgttgaagcg gggttccggg aagggcatat
     7621 caaggcgatc gcctcgaccc cgacgctcgc ggccggcctg aacctgcccg cccggcgggt
     7681 gatcgtccgc gactacctcc ggttcaacgc cggggagggg atggtcccga tcccggtccg
     7741 ggagtaccgg cagatggccg ggcgggccgg gcggccgcac ctcgacccct acggagaggc
     7801 cgtcctgatc gccaagtccg aggagatggt cgaggagctc ttcgactgct acatcggggc
     7861 gcctgccgag gacgtccgga gccggtgcgc gaacgaggcg gtcctctgca cccacatcct
     7921 ctcgctcatc gctaccgggt tcgcccgcga gacgggcgag gtgctggggt tcatggacaa
     7981 aacgttctac gcccaccagg gcgagagccc gctcgcgctc tcgcgggcgg tcaacagggt
     8041 gcttgagttc ctggaggagg cggagatgat caccgaggtg ggggagtggc tcgaggctac
     8101 cgagtacggg tcgctcgtct cccggctcta catcgacccc cgaagcgccg agacgatcgt
     8161 gacgacgatg gcccggcaga aggagtacac cgatatcggt ctcctgcacc tcctctgctc
     8221 cacaccggat atgatgacac tcttcgtcag gagggacgat atgtatctcc tggaccggtt
     8281 cctcgccgac caccgggacg acctctggat ggagatcccc tgggacgcgg gcgaggggtt
     8341 cgaccggagc ctcaagacgg cgctcctcct cgccgattgg gccgatgagg tgggcgaaga
     8401 cgtgatctgc gagcgctaca acgtcggccc tggcgacgtc tacgggatgg tggagagcgt
     8461 cgcctggctg gtccacgcga cccggcaact cgccggtctc ttcgccccgc acctcgccgg
     8521 tccgatcgag gagatggaac tccggaccaa acacggcatc aagaaggaac tcctgcccct
     8581 catcaggctc cgggggatcg gccgggtccg ggcccgccgg ctcttcaaca gcggtatcgg
     8641 gtccctcgag gcgctccgcg ccgccggccc ggagaaggtc gggacggttc tcgggcaggg
     8701 gatcgccgct cagatcttcg agcagctcga ggggagacgc gacgagatcg ccggggccac
     8761 cggcgagcag tcgaccctct cccggttcgg gtgaactaaa aatgacagaa attgcatgcg
     8821 atatctaccc ggcggtcttt acggtcgacg agaacgtcag gttcctccgg gagatccgca
     8881 gggtagccga cgagtgtggc actcacatca tcctcttcga cgccgaccgc ctcgctggaa
     8941 gggaccacgt tgatgcagca ctccggcacg cctggcgatc gtgggccggc ggcgagccga
     9001 tagcgaactc tatcgagatg gaggcgctcc tctacgccgc gggaacccgg cagtgccagg
     9061 tggccgcatc cttcgggatc catccggggg agaaccactc ctacatcgcc gtctgcccgc
     9121 cggcgccggg tgtccgggac cgccttgccg atctcgtgac gttcgtcgat gctggccggg
     9181 aggaggagat cgatcccgca aaaagggcgc gccttgcgga ggtcttcggg atcacccccg
     9241 aagaggtcct tgtggtcggg gaggcgcggt tccgcgacct ggtcctcgaa cgggtggcgc
     9301 tcctcgacgt ctaccgctga cccgacgacg cgtgggtgag gatgtgccgg atctcgggga
     9361 tgatgatctc tttggcggcg agcgtgacgg ccttcgtgga tcccgggatg cagaagaccg
     9421 cccggccgtc gatgaccccg gcgatcgccc gcgagaggat cgcggcggtc ccgatctctt
     9481 cgtaactttt gaggcggaag agttccccaa acccgtcgat cgtcttatcg agcagcggca
     9541 cgaccgcctc gatggtcacg tcgtcggggg tgagcccggt gcccccggtg atgatgacgc
     9601 aggttgcacg cgaaagcgcg acagggactt cggagcggat ccgcgcgata tcgtcgggga
     9661 tcacggcgta atgggtcacc tcgattcctg cggcagagag gaggtcgatg agcgtctttc
     9721 cgctggtatc ggtctctggt ttccggctcg aagagacggt gatgacggca ccggtgaccg
     9781 tgaggggctt aagatggtta ctctccatag gatcttccag tacccctgta tcccgtattt
     9841 tacttctttc tatcccccga tttgaggggt tcaatccccg acccctttgt ttcgactgga
     9901 actacaggga ggcatgacgg tctcccggaa ggggtgggag ggtgtggtcg agagagcaag
     9961 gcggggttcc cccggatctc tgatgataga atctcgttat ttggagccag ttgagagaat
    10021 ccgggctgga taggggatct gctcaacatg ataggccgct ctcatctcgg gagggttgat
    10081 ctgattcacc aggagaggaa accggatcct ggtggaatgg tgatcagatt cacgcgggag
    10141 ggtaaccctt ctcatcctgt tctccttcct cccactccgt actcttctta tcttgtcctg
    10201 ttctccttcc ttcctacccc atactccata ctccatattc cgtactcttc ttatctcatc
    10261 ctgttctcct ttctccccgt cctgtacctg tctgatcctg ttctattcaa accctgtaca
    10321 cttaccataa aactttatat cttgagttgc agaggtacac atgattatta tcatcacctt
    10381 tgatttgatg atacaccctg tgtatcatca tcaccctcca cacgaaacaa aggggtgggg
    10441 gtgatcgccc cttcccctcc ccgggaacac tctccaacat catccattct gccatccgtc
    10501 agcagtctgg tgttccactg gaaacgaagg ggttatctgc cctgaaagcg atcctattaa
    10561 gagcccttga cacgaaaatt tcctgtcctg gcaacctcct ccggtgacac catgtcggac
    10621 gataagacaa acccaatggg actattcaaa aaatacctga ccaatagacg tatatttaag
    10681 aacagggaag tgctccgaca cagttatcgt cctcagatcc tgcctcaccg gaaaccgcag
    10741 atcgatgaac tcgcctccat ccttgcgcct gccctcacga acgagacccc gtcaaacatc
    10801 ctcatctacg gcaagaccgg gaccgggaag acggcgtcgg cccggtacgt cgggacggag
    10861 ctcgagaacg ccagtgcact cgccgggacg agatgcagga tcgtccacct caactgtgan
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn cagcggtggg gtcacggtca tcatcctcga cgagatcgac
    11041 aaactcgtca agaagagcgg cgacgacacc ctctacaacc tcacccggat caactccgat
    11101 ctgaagtctg ccaaagtcag catcatcggg atctccaacg acctccggtt caccgacttc
    11161 ctggaccccc gtgtcctctc ctcgctctcc gaggaggaga tcgtcttccc cccctacaac
    11221 gccccgcagc tctgcgacat cctccagcag agggccgagc tggcgttcgt ggagggagcg
    11281 ctcggcgaga cggtcatccc gctctgcgcg gccctcgcgg cgcaggagca cggcgacgcc
    11341 cggcgggcgc tcgatcttct ccgggtctcg ggcgaactct cagaccgcga aaacgccacg
    11401 agcgtggatg agaagcatgt ccggatggcg caggagaaga ttgagaccga cagcatggtg
    11461 gagtgcatct ccaccctccc gacccagagc aaggcggtcc tctactcgat gctgatcctc
    11521 gatcagatgg gcaaacggat cttcacgagc ggcgaggtca cggtggtcta ccgtgagatc
    11581 gcccggatca tcgacctcga cgtcctgacg caccgccgga tcacggatct catctccgaa
    11641 ctcaacatgc tcggcgtcat caacacccgg gtcatnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nctcctggag gagaactgcc gcaccccgtc aagcgagctc gcggtgctgg ccgaggtgag
    11881 cgagcaggat gcaaaagatc ggatcggccg gttggaagcg accggtgcca tccgccgcta
    11941 cggggcagtc gtcgactggg agcgggccgg tgacgggaac gtcggcgcgg tgatcgaact
    12001 gaaggtatcg cccgagcgtg actttggcta cgaccggatc gcggaacgga tcacccgatt
    12061 cccgcaggtc cggtcgctcc ggctgatgac cgggacttat gacctccagc tgctggtgac
    12121 cgggccgagc atgcacgaga tcgcccggtt cgtagccgag cagatcgctc cgatggaccg
    12181 gatccgcgag acggcgacgc atatcatcat gaagacctac aaggagaacg gcaccacctt
    12241 cgccgagcgc gaagaggttg agcgtctccc ctactcgttc tgaggtgaga tgtgtgagga
    12301 acttcgtctc ggaacgggcc cgcgccatac ctccctcagg aatcagacgg tttttcgata
    12361 tcgcccagac gatggaggat gtcatatccc tgggtgtcgg cgaaccggac ttcgtgaccc
    12421 cctggtgcgt ctgcgaggca tccatctact ccatcgagca gggatcgacc gcctacacct
    12481 cgaataaggg aacaccccag ctccgtggcg ccatcagccg ctacctcgat acccggttcg
    12541 gtacacgcta cgaccccgaa aatgagatca tcgtgacctg cggcgtctcg gaagcggccg
    12601 atatcgccat ccgggccgtc gtggacccgg gcgacgaggt gctggtcgcc gagccgagct
    12661 acgtctctta caccccctgc gtctccctcg ccggcggggt cccggtgacc gtcccctgcc
    12721 gggcggagga cgagtttcgg ctgacggccg atgccctcgc ggagagcatc accccgcgga
    12781 cgaaggtcct gatagcaaac ttccccaaca accccaccgg cggggtgatg cagaaggcgg
    12841 actggcatgg aatcgccgat ctcctggttg ataaagacct cctcctcatc agcgacgagg
    12901 tctacgccga gctcacctac gacggcgacc acttttcgcc agcgacgatc cccgaactcc
    12961 gtgatcggac gatcaccttg aacgggttct cgaaggcctt cgccatgacc gggtggcgga
    13021 tcggctacct ctgtgccccc ccggagatca ctgcggcggc cctgaagatc caccagtacg
    13081 tcgcgctctg cgcgccagtc atggggcaga tcgcggccta cgaggcgctc cggatcgggg
    13141 gagctgagaa ggacgccatg gtccgggagt accggctccg gcggaacctc tttgttgacg
    13201 ggttgaacaa actcggtctt gcctgccacg ttccgaaggg cgcgttctac gccttcccct
    13261 cggtggagag caccggcatg accgataccg agtttgccga gtcgctcctc agtgagcagc
    13321 atgtggcggt cgtccccggg agcgtcctcg ggtcctccgg tgccgggcat gtgcggtgcg
    13381 cctatgcggt ctcgcgcgac aacctcaaag aggccctctc caggatggga gcctttctgg
    13441 actcggtgaa ataagaacgt catcataagc gccctccaaa caaaataaaa acatttttat
    13501 caggcatctt tgtttaagtg acaggcggtt gggtgtccga caggagaatg gcgggcagac
    13561 aaccccctta cttccactgg agatgtggat ttattcaggg attctgactg cccgggatcc
    13621 cgccacaccc atactcagaa tatgcatcct gtcctccaaa ccccgggcag atcttcgggt
    13681 tcgcatccgc gctccggcaa acgatctaat tacagatcag catgccgctc caggggaaaa
    13741 actcgatgcg gcggtcttgg atgtcccgga aattaaattt ccactggaaa tgaaggggtt
    13801 cagattccga tggcaaccgc gccggagaca ctctgcggca gcgaaggttg atatacgatg
    13861 cccgttaaca gccgggaatg cggaatatac gcgaatattg tggcgcaacg ccagagacac
    13921 ctgccggcac tccacacctc ccgcgagacc cgtccaacca caggggatgc ctccagttgc
    13981 ctgcgtcccg gtgcccactc ccggggcgcg caacagcgcg aagcgagcgg tacggggact
    14041 gtcggacccc ggctgccttt aaagggtctg accgctcacc cggtatcaca ttgtaacgtt
    14101 tatcacccag tatcactcca tcctacttga tagaacaatg agttgtacca ttatcgtcgg
    14161 cgggttcttt ggtgacgaag ggaaaggaaa gatcgtagcc catatcgctc accaggatag
    14221 accatccatc atctctcggg gcggcgtcgg tccgaacgca gggcataccg tctgtatcgg
    14281 agagaaggaa tacggtgtca ggatggtccc ctcgggattc gtctacccgg aagcggccct
    14341 tatggtcggg agcggcgtgc tcgttgaccc ccgggtcttc ctgcgggagg tcgacttgct
    14401 cggcgtccgg gacaggacct ttgtcgatgg gcggtgcggc gtcatcgaag aggaccacat
    14461 cgcgcgggac aaagcgagcg cgcacctcag ggataagatc gggagcaccg ggaccgggtg
    14521 cgggccggcc aattcggatc gcgttctgcg gacatcccgg caggcgaaag atgtcccgga
    14581 gctggcagac tttatcaccg atgtggccga ggaggtcaac tgcgccctgg ataaaggtga
    14641 ggtggtcctc ctcgagggga cccagggctt cgggatctcg ctctacttcg gaacctatcc
    14701 attcgtgacc agcaaggata cctccgcctc gcagatcgcc gccgacaacg gcgttggccc
    14761 gacccggatc gacgacgtca tcgtcgtctt caaggcctat ccgacccgtg tcggtgaggg
    14821 gcccttctcc accgagatgc cggtggagcg gtcacacgag ctcggtatcg aggagttcgg
    14881 tactgtcacc catcgccagc gccgcatcgg cacctgggat gggaagatgg cccgctactc
    14941 ggcgatgatc aacgggtgca cccaggcggc gatcaccggc atcgaccgga tcgacccggc
    15001 ctgtttcggc gcgacggact acagtcagct gaccgcgaag gcgaaggact tcatcgttca
    15061 ggccgaaaag gacatcggca agcccgtcac cctgatctcg acgggtcccg agatgtccca
    15121 gatcatcgat ctccggggca ggcgatgaga cggaacctga tggatattct ctgctgcccg
    15181 gtctgcaagg gcgacctcct gctgacggtg accgaagaga acggtgagga ggtgctggag
    15241 ggcaccctcc ggtgcgcgac ctgttgtgtc gattacccta tcagtgaagg catcccgaac
    15301 ctccttccac agaccgccgg tgaggactga tctcgtcgat gtcggagatt catctcaacc
    15361 gccgggggat taacacgatc gaggtcccgg acgtggtcga ggcgatgccc gggagcgacc
    15421 tcgccctcag gctcgtcaac catggctcgc cgctccacat cactctcgcc tcagcgaact
    15481 cctcgacctt caccggtttt tttcacgaga acctttacgt gaacgggacc gaggagttca
    15541 ccatccccat ccgggaggac gcctaccccg ggacctttaa cgtggaggtc atcgccgggt
    15601 acggcgcccg gagagcggag ttccgggtcg tcgttcggga acgggcgaaa tctgagctcg
    15661 agccggttga gtcccctccg acagcggccg tcacgggtgt ctcgtctcgg tggcggctct
    15721 ccgctccggt cttcgccctc atcgccgctg ccctggtcct ctacgggctg tggctggcct
    15781 accgggttga gctctttaac gccgctgcgt ttgcggcgct gctcctcggg gtcatcttca
    15841 catggctacg gcagcggtcg taatcgctgc gtcgctgctg gtggatcggc tcatcgggga
    15901 cccgcattcc cggtatcatc cggtggcggt cctcggccgg ttcatcggat ggtggggagt
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctggtacc tctacctcct cctcgcgccc
    16021 ctcctcctca aggcctgtct ggcctggcgg gcgctcgagg agcataccgt cgccgtggta
    16081 cgggcacttg agaccggcag catcgatgcg ggacgttcac aggtcgggat gatggtgagc
    16141 cgggatacgg cgcgcctcga gcgcgagcag atcctctcgg ccgcttacga gtcgatgacc
    16201 gagaacctgg tggacagcat catctctccg ctcttctact tcgggctctt cggccttgcc
    16261 ggggcggccg ctttccgggc ggtcaacact ctggatgcaa tgctcggcta ccgggacgag
    16321 cggctccgga tcgggtggtt cccggcacgc gccgacgacg tcgcgaactt catcccggcc
    16381 cggatcaccg gttttatcct gctcgcctac tttgcagcga agggccggtt cgccccggcc
    16441 tacgcggcgc tccgccggga tcgaaagaag cgccccggat tcaacggcgg gatcccgatg
    16501 gccgtcatcg ccggcggcgt cgggatcagg cttgagaagc ccggcgttta cacgataggg
    16561 aacccggagc ggacgcttgc ggaggcgggc gccgggatcg tccgtgcggt ccgggcggcg
    16621 acgatcatct tcgccctggc ggagatcgtc gcactatttt tattgtggtc ggtgagctat
    16681 acatagaagt aatgaaactc gaggaactga ggtttggcac cgagctgatc aagcgcggct
    16741 ttgcatcgat gcagaagggc ggcgtcatca tggacgtggt caatgcagag caggcgcggc
    16801 tcgccgaaga ggccggcgcc gtcgccgtta tggcgcttga gagagtccct gccgatatca
    16861 gagcggccgg gggcgtggcc cgcatggccg acccgcagaa gattgtcgag atcatcgatg
    16921 cggtctccat cccggtgatg gggaaggccc ggatcggtca tttcgtcgag gcacagatcc
    16981 tggaagcgct cggtgtcgat atgatcgacg agagcgaggt gctgaccccg gcggacgagc
    17041 agttccatat tgataaggcc ggatttggtg tcccgttcgt ctgcggcgcc cggaacctcg
    17101 gggaggcgct gcgccgggtc aacgaggggg ctgcgatgat ccggacgaaa ggcgaggccg
    17161 gcaccggcaa cgtcgtggaa gcggttcgcc acatgcgggc gatcatgggc gggatccggg
    17221 cgctgaaagg tctctccagc caggagctca tcgaccgcgc ccgcgagatc gaggcacctg
    17281 cggaactcgt catcgagtgc gtgaacctcg ggcggctccc ggtggtgaac ttctcggccg
    17341 gcggcatcgc gacgcccgcc gacgcggccc tgatgatgca gcttggggcc gacggtgtct
    17401 tcgtcgggtc gggcatcttc aagtccgcaa atcccgggat gaccgcccgg gcgatcgtgg
    17461 aggcggtgaa ccactacaat gagccgatgg tcatcgccga ggtgagcaaa ggtcttggcg
    17521 aggcgatgaa aggcctcgat gtccacctgc tccgcgaaga cgaggtgttg cagacccgtg
    17581 ggcgttaagg tcggtgtcct cgcgctccag ggcgacatcg ccgagcatat tctggcgttc
    17641 cgcgaggctc tcgcggaccg gccgggttca acggtcatcc cggtccggca cgtggaggac
    17701 ctcccggggc tcgacgccct cgcgatgccg ggcggcgagt ccaccaccat ctcccggctg
    17761 atcgggaaga acgggcttta tgagccgctc gccgggttcg agggcgcgat atttgcgacc
    17821 tgcgccggga tggtcctcgt ggctgaccgg gtggacgacc cccgcgtccg gaccctgggt
    17881 ctcatcccga tgcatgtagc gaggaacgcc ttcgggcggc aggtcgactc ccgcgaggct
    17941 cttttagatg tagcgggcct cgccgatccc ttccgggcgg tcttcatccg ggccccggtg
    18001 gcgacggaag tcgatcccgg tgtcgaggtg ctcgcccgca taccggaggg gatcgcggcg
    18061 gtcaggagcg gccggcacat ggcgttcgcc ttccacccgg agctcggcgg tgacctgcgc
    18121 ctgcaccgga tgttcttgga gaccctcggg gtataggacc cgggattcac ccggttcttc
    18181 gacttccggc gtcgcctatc acctgtcttg cccgggccgt gccatcggcg gtcagccggt
    18241 agatgatcca gtttccctgc gcctcgccgg cgatcagtcc cgtcttcttc aggacggaga
    18301 gatgatagga gagcttcgag tccgctatcc cgagcaccgc cttgatgacg cagacacaga
    18361 gcggctgcac ggcaagcatg gcgaggatct tgaggcggag ggggtcggcg agtgcgcgaa
    18421 aaagagcact ctctcttacc aggagatcgt ctcccggcaa ctgccccgtc agccctgcga
    18481 tgcccccgca ccggcagagc gactcctcga cctccggcgg gaggggcagc gtctcttccc
    18541 ggcactctct cttctccgtc actgcatcat cttcctgctt caaactgatc tgaaatgaga
    18601 cggcatatac cttttccgca ccttcgtacg ctccgtcgcc cggcgtgttt cgctcccgag
    18661 cgcgtgccct ctcgcacagg aaccgggagc gataccagtc accaatcacc cccgatgggt
    18721 caggcagagg tattccggca gggccgtctt gctgcatccg acaccggaca ccacaacctt
    18781 ttttttacct gaactcgtac acattaattc aaattaattt gaattgctgg gtcccggtct
    18841 tccagcgagg tacacgcatg atcgacatac tcatcaacgc tctctcgtca gggctcgccg
    18901 cagtgctcga ctacctctcg gcgcacgtcc tcacctgcct ggtgccggcg ttcttcatcg
    18961 ccggggccat cgcggctttc gtcaagaagg acgcgatcct gaagtacttc agcccggaca
    19021 cgcccaaaca catctcctac gggatcgcgt cggtctcggg gacgatcctc gcggtctgca
    19081 gctgcaccat cctcccgatc tttgccggca tcctcaagaa agggagcggc atcggcccgg
    19141 caacgacgtt cctctttgcc ggtcccgcta tcaacatcct cgcgatcatc tacaccgcat
    19201 gggtgctcgg gttcgacctc gggatagccc gggcgatctc ggcggttctg ctcgccatcg
    19261 tcatcgggct tgccatggcg ctgatcttcc ggtcgacgga cgttgaaacg ctcaaaaaga
    19321 aggcgatggc gccgagcgtc gccggggcgt gcgaggaaga gaaaccgcgc tgggtcacgc
    19381 cagcgttctt cgccctcctg gtcggggtgc tggtcttcgg cgcctcgccg ctcgactgga
    19441 cgctccggct cggcatcgtc tacgtcctga ctcttgcgat cgccgtcctc ctcatctact
    19501 acttcgataa ggacgaggtg accgactggg ggatggagac ctgggacctg acaaagaaga
    19561 tcttccccat cctgatcgcg ggaaccttcg tcgtcgggat catcgccttc ttcctcccgc
    19621 cggagacctt ccagccctac ttcgggaaca actcgctcgg cgcgaacttc cttgcggcga
    19681 ttgtcgggat gatcctctac atgccgacgc tgcttgaggt cccgatcatc ggcacgacgt
    19741 tcggctacac caccgggcac atggccgcag gaccggcgct cgccctcctg ctggccgggc
    19801 cgacggtcag cctcccctcc gtcctcgtca tctatcggat catgggggcg aagaagacgg
    19861 cggcctacgt ggcgctggtg gtcattttcg cgaccattgc cgggttcgtc tacggcaacg
    19921 ggatgctgct gatataggag agagcgtatg gagacgaaga agagagaaaa accctgctgc
    19981 gctgccgaag ccctgcggcg cattcgccgg atcgaggtcg ggggggtcgt cgtcgggctc
    20041 gcgatgctcg acgacgtcct ggccgacgtc atcgagcacg gcatcactgg cgttaccgct
    20101 atcggcgatg aactgatgag acagataaag atctacaact atatcccgaa ggaggcggaa
    20161 ccgctctaca gagcggcgct ccttttagcg tatcagaacg aggtgaagaa acgatgaagg
    20221 ttgaagtgct cggaaccggc tgcgccaaat gcaagcggct cgcaaagaac gtagagacag
    20281 cgatcaacga tctcggtatc gaggccgaac tcgtcaaggt cgacgacatc accgagatca
    20341 tggaccgcgg cgtgatgctg acccctgccc tcgccgtcga cggggaactg aaggtctccg
    20401 gccgggtggc ggacgtcaag gagatcaagg agatcttgca ggggtaagcc gcccttcccc
    20461 gcgcggagga agaatggtgc tgatagaagt attcgggacc ggctgcgtca agtgcaagcg
    20521 gatgaacaag aacgtcgaga tcgcagtcag ggctctcaaa atagatgcgg aagtccacaa
    20581 gattgaagat ctcgatgcaa tgatcgaacg gggggtgatg ctgacgccgg cgctctacgt
    20641 cgacggcgag gccaaagtcg tcggccacgt cccctgtgtc gaggatatca aacagatact
    20701 gctcggagtg aagtgacatg acagaacaga cagtgccccg gtgtacgtgc gggggtgacg
    20761 cggagccggc aggcggcacg aaacggatca tcttcgcctg cgccggcgcg tcgaaggccg
    20821 ggcagctctc gaatgaggct gccgtttggt taacgaagga aggatacggc aatatcgcct
    20881 gcacggcatc cctcgctgtc tctacaccct cgatcaaaaa gaaggtcgag gaggccgacg
    20941 aggtggttgt catcgacggc tgcccggtcg gctgcgcccg gcagattgcc gagcgggccg
    21001 gggttgtacc cgaccaggag atcgtggtca cggctctcgg gatcgagaag ggcgggagcc
    21061 tcgacctctc cgaagaggac gtcgagacgg tcgtctccgc cgcctgggag ggacggggaa
    21121 gagagaagac agagaaagcg cgcaggccac ccggtggatg cggctgcggc ggcggctgct
    21181 gctagggtga gggcatggcg gcaccgaaac cggaacggcg cctctccacc ttcgagaagt
    21241 acctcacgat ctgggtggcg ctctgcatcg ttgccgggat cctcctcggc agcaccttcc
    21301 ccggtgtcgc ggtcgcgctc gacgccttct cggtctacca gatctcgatc ccgatagcca
    21361 tcgccctctt cttcatgatc tacccgatca tggtgaagat cgactttggc gaggtgctcc
    21421 gggccgcaaa gaccccaaaa cccgtcgccc tcacgctcct cgtgaactgg gcgatcaagc
    21481 ccttcacgat gtacctcatc gctaccttct tcctcggcta cctcttcgtc gacttcctcc
    21541 cgggcaccga gatcctcccg aacggcgtcg aggtcgagct ctggcggagt tacgtcgccg
    21601 ggtgtatcct gctcggcatc gccccctgca cggcgatggt gctgatgtgg agttacctcg
    21661 cacggggcaa cgacggcctg accctcgtca tggtcgcgat caactcgctc acgatgctcg
    21721 tcctctacgc cccgctcggc gggtttcttc tcggcgtcgc cgcgataccg atcccctggg
    21781 agacgatcct cctctcggtc gcggtctacg tcggcctccc gctcgcggcg ggctacgtga
    21841 cgaggaagtg gatcatcgcc cggaagggca tggcctggtt cgagacgagg ttcctgcact
    21901 acctgactcc ggtgagcatc gccgccctcc tcg
//
LOCUS       DAJB01000226           21004 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_28735, whole genome shotgun sequence.
ACCESSION   DAJB01000226 DAJB01000000
VERSION     DAJB01000226.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21004)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21004)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21004
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_28735"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    12571..12584
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4413 a         6522 c         6361 g         3694 t
ORIGIN      
        1 aacggcatat cagtccttga tgagagcaaa tgcgtccagt gcggcgtttg cgtccactcc
       61 tgcccctatc atctcctgaa atccgagtat gcccactacc agatcacgat cggcggccgg
      121 cgcggagcct cccccgccgc aggaagggaa ctcgtcaccg tcgagaccga ggaggaggtc
      181 gtcgaggtgg tcgaccgtat cgtctactgg atctaccgca acgcctggag cggccggcct
      241 ctcgccgacc agatggacga gatcgggctt gacaaattcg cggaggggat ccaaaaagag
      301 ttcgggccga aggaagaggc ggccggggag tgagaaccgg cctcctacac aggcatcatc
      361 gctatacccg atatcgagag tacaagcacg atgagcagga ggaggtcact cctcccgggg
      421 cggggagggc gatagcaggt gggccttgcg gtcgaggcga accccctgct ctgcatcgct
      481 tcagtcacgt ctcgggcccg gttcaggacc gataccgtca tggggatgcc gagcgccatc
      541 agttttctcc tgtttttgat gaacgtcccc cggtcgaact caagccctct ggaacgctgg
      601 gcatccatga tatttctgag atcgtcgtag agggccgtca tcgacttcag cgcataccca
      661 agggagaaag cgatggtcgg aggcagcctg ctggcacgga ggctggcgac gaaatcagtg
      721 tacgaagtgg tcatcacgaa gagaaacgac atacccccga tgacaaggac tctgattccc
      781 tttgccgcag catagagcag cccgccggag gttatcaccg atgagaacgg gccgatctgg
      841 gccgaaagat acacgtaccc ccaggtctcc tcgggaaaga agatatcgac cgcgacgatg
      901 aagatgagga gcggcaggag gaggagaagc gcccgaaaaa gcggtctcgc gagtcctgcg
      961 gcagccgcaa ggcaggcaac gaaggcaaag aggaatgcag tgcggacggg cgacgtcgtg
     1021 gcaaacgcgg cgatactcat cgcaagcacg gtcaggagtt tggtcctcgg gtccatccgg
     1081 tgaaggaacg aatccgccgg gatgtagccc actgcccggt aggcatgcat ccgatcggac
     1141 cgtttgcggc cggcagtcat ccctgctcct cctgcactct gcggtcgtcc acgatgcggc
     1201 cgcgctcgat cctgacgatc ctgtccgcga ctgcggcgga gaactctgcg tcgtgagtcg
     1261 cgacgacgac cgcctttcct ccggcgcaga gcgtatcgat gagcccggcc agcctctgtt
     1321 tggcaacggg gtcgagcccg agcgtcggtt cgtcaagcgc caggacgggc gtatccatcg
     1381 tcaggacgga cgcgacgctg acgcgttgct tttctccgac cgagagggca agcggcgaga
     1441 cggtaagtcc cagatgcctg atgccgagat gctcgcgcac gtgctcgatc cgctgcgtta
     1501 tcacgtccgg aggagtgccg aggtttgaag gggcgaaagc gagttcctcg gcgatggtct
     1561 ccccgaagag ctggtagtcg gcatgctggc agaccaggcc gacggtgcgg gcgatctcgg
     1621 cgacggtgct ctcccggatt tctcttccgt ccacgacgat tcgcccggac tccggccgca
     1681 gaagaccgtt taagagtttc agcaacgtgc tcttccccga gccgttcgga ccggagagga
     1741 ccgtcacctc gccggggtag atagccagag agacaccgtc cattgccggg agatctctac
     1801 ccgggtgccg gaacgtgacg ttctccaggg tgacgatacc ggcgcaggca gcccttgccc
     1861 tccggcgggg agggcggtcc cttgggagag catccggtgc gggaggctcg atcaaccttc
     1921 cgtcgacaag ggatatccgg cgggatgccc accgcgcgac aagttctgtc cggtgttcgg
     1981 agaggatgat cgtcaggccg gacgtccggt tcaatgtatc gaggagatcg aggagcccgc
     2041 ttgcactcgc ggcgtcaagc ccggagaacg gttcgtcaag gatgaggatt gcagggtgca
     2101 gaacaacgac ggaggcgatc gccagacgct ggcgttctcc ccaggagagt tcggtcgtct
     2161 gccggtcgcg aagtgggtca agccggaact ctttcaggat ctcggtgacc gcccggtcaa
     2221 tccttgatgc ggaccagccg agctgttcgg ggccgaacgc aatctcgctc accacatcgt
     2281 ttgagaagat ctggtgatcc gggtcctgaa agaccatccc gacagtggtg gcaaggtcgc
     2341 tgatcgtgca gtcgcgcgta ttctgtcccc ggacaatgac atcccccttg aaggtaccgg
     2401 gccgagcgtg cgggatcagg ccgttgcagc accggaggaa ggtcgtcttc ccggagccgc
     2461 tcggcccggt cagcagcaca cgctcgccct cccggatggt acagtcgatc ccgctgagca
     2521 cttcccgcgg ccgggcagac cgaaacgcca ggtgccggaa cgagaccgac ctgaactcga
     2581 tcgccgggtc agccctcatc cggcacatcg tcctggtgca caatccccgc cctgtagagc
     2641 cgtccgacga tcagcgcggc gatgataccc ccgatgcccc cgaagaccag gtgcgtgaac
     2701 gacgagaccc cgatgccgag gaggatgaag acaccctgga cgccgaggag caggtgtacg
     2761 gcatagttca ccaccatctt caccatgttc cccgcggcgc cggcaatgaa cccgacgacc
     2821 gggttgtcga ggcggtggcc gaacgccatg gagacaaggt cgatggtgac ccccattgcg
     2881 gtatacttga agatatcaaa gacatgaagc ggttcaacgc cgaagaacga cgcgagaatg
     2941 ccggagacca gtccggcgta cgtacccgct cccggtttcc gcacgagggc ggcgccgacg
     3001 acgagcggca gaacccagaa tatccccgaa ctcccggggg tgtggatcgg aaccacgatc
     3061 gccatccgca ggacgaagac gagagctccg aagagcgaga gcagggcgat ctcgtacagg
     3121 ctgaagtaag aagagcgttt catcatcggt tatcctccac aaacgtcgat tcgagcgtga
     3181 gtccgaaatc ctctggctgc ggccggtcca cggcgaccat caggaagagg tccccgaccc
     3241 tgttgccgcc ggtatacgtg acatagaacg atcccccgga atcggcggtc tcggtcacct
     3301 gccctttccg ggccgcaaga tcggtggtga tgtggatacc gtccaggcca gaactgatcc
     3361 gctcgaacca gaggacgcgg gtatccctgc ccacgtcctc gtccaccggc cgcggggaac
     3421 ccgctacctc gacggtatat gtgttgccct ccgggagagg agacatcccc catggcgaat
     3481 ctccgggcgc agcggtcccg gtgacgtagt agccggaggt gccgtagccg gactgccggt
     3541 tcgtccacgc cctgccgtct ccggcgtgga gaccgtagac ggaaaacgac gccgttccgg
     3601 caagatccgt gaactgataa tcgactttga ccgagccggt ccggaagagg gccgggttcc
     3661 cgccgacagg caccactccc aggtctacga aggcaagata ccagctcccg gaggggagat
     3721 cgcccggacc gatcgcgatc ggtgtggcgt agggccgcca tgactgggga ccgtaccgga
     3781 gatcatagcg atcgatggtt gcggtatgca ggagagagtc agtcgaaccg gtctccgggg
     3841 cataggcccc cgagacggct gtatcgatca ggaacgaaac agcgatgact gccccgagcg
     3901 cgacgaccgc aagtatgact ctcctgccga actccatgat cagtctcttt tcaccgcaat
     3961 cagttccccg tcatgctcat ccacaaaaag gaccgccctg ccattgatgg ggcaggcttt
     4021 ggcggccttg ccctctttta acacaatcat cccttcggta acccgccggc cgtcaagcgt
     4081 catgatctcg tcgatccctg cgccgaggag gacatccgcc ggtgtcgggc cggtgtggct
     4141 gcggatggcg cctttctgga ggaacctgcc gtcgttttgt gtcgctgcga gcccgaggcc
     4201 cgccatcgca ccgatcacgc cgtcttcggt gccgccgagg ccttttaacc atatgccgag
     4261 gttctttgcc agcgtccggg cgcgtgcctg ggtgatcacc atctgctggg catcgcggcc
     4321 gaatgctatc acggccgggg ctacctggtc gagggtcgct ccggcaaggc cggggtcgct
     4381 cccctcgatg aagtcgtcga gcatcgtctc ctcggcaatg gagaggcacg tatcgagcgg
     4441 ctcacgaccg tcggcatcga cgtggatgac cgcgcagctg ttgtgggacg tgaacgggat
     4501 cgaggggtgg acaaagagtt ggtgccgggt gacaccccac acagggtagt gtttctcgat
     4561 cgcgtcggcg accgcacggg ccaggcggcc cgtcccccgg gattccaggc tgtccgtatc
     4621 gtcaagagca agaagtaggg tcatgatatg tctttttttc ccgaatagac tggggaggtg
     4681 aagataaaaa aaggtgctga aatcaggccc atagcaatta ggtttgatta acctgatcta
     4741 aaataatgat ctcaggttaa ctccccaccg acagcgctgc tgaactaacc ctgccgtacg
     4801 atggcttcta tcgtccggat aactgcctgg aacccctctt ctcggggcgg tgatccggca
     4861 taggagacgg ctgcacactg ttccaggatc gaagataccg tcccggcctc acgaccgctc
     4921 tcctcgatca ggcgcagcac gtcaccgtcc gcgagttctg ctccggtccc gtatgcagac
     4981 gatacgtacg accgcagcgc cctgccggcc gtaccgtacg cctccttccg gtcgccacgg
     5041 tcgaactgcc tgcgtgcctc atcgaggagg tgcagggcaa cgcttgaggg ggcgcagggg
     5101 accggcaaag cgaacggctc cggcgttttc ggggctcccc gccggacgag gtacgcgatc
     5161 gcgaggatga tgcagagcgt gcctgcaccg gcaagccaca tgaagtcaaa cggctcttcg
     5221 agatccactc cccgcacctc ccccccgatg agagtggcga tgatcctcgc ggtccggttc
     5281 ccgccgtcgg taaatcccac gctgacgaag gcagctcccg gtgttgcatt catcgaagat
     5341 gcggccggga agaagccggc atctgcaagc tcgacggcat actgtttgta ggtatcattc
     5401 gccgcgaccg catcaggcac cacgagcgac gtgccggtgg cgtacacggc atacgccgcg
     5461 gtaccgttct tcaccacacc gcctgcgcgt accgtatccc cggtacctcc gcggtactca
     5521 aagacaaacg ttccgccgtc ttcgccgatg ggatcgatgg tcgtcgttgc ccgggtatag
     5581 ccatgcgacg tgaggacggc gtggatctcc tttaccaccg ggtcgtcctc gagcccggcg
     5641 agcagtgcgg accgcagggt ctgcatgcgg tcctgctccc gcgccgcctg ctcctggagc
     5701 gagcccatat cctgcgagat ctggccgggt tgtttggagg aggatgagga gggcgccccg
     5761 ccggaccctg cctgggtcga gacggacgag acggaactca cctgccggtt ctgctggtcc
     5821 tcctcctgtg cagccgaact gacgacatgg acccggatct ccggcagggc gatccgggtg
     5881 cgcggcgcgt tcccgtactc gaatgcgacg tcgaagaaga acgtgccggg cattgccgcc
     5941 gttccggcac cgacggctat ctcccgggtg tcccggaaga tggtgatcgc ccgggactgg
     6001 gactgggtcg ccggactgct cccgtcaccg ccctccggaa cgaaccgcac ctcccggacg
     6061 agcgtgccgg tggtatcggc gccgagcgta ttctcgacgg cgaccgggaa cgaggcctcc
     6121 ccgccgacct gcacggtgta gtcggactgt ccggtcgaga aggtgatacc ggcagccgcg
     6181 gcggtcgcca cgcagccgag ggtaaccagg atggcgagaa ggagaactct cctcacggca
     6241 ggatcctccc ccggccatag cggaggtaga tctccagcag gagaaccccg atcgctccgg
     6301 cgaagaagac ctcacggaca ctcgtatctt tgggctcacg cgggatctcg tcggtcagcc
     6361 cggcgtagat cgctacaagc gtcccttcat cggccgccgc atagtactcc cctcccgtcg
     6421 tgcgggcaat tgccctgagc gtctcttcat cgagcgcagc agactcggtc gtacccgtca
     6481 ccgggcccgt tgagccgatg cctatggtgt agaccggcac gctccgttcg caggcgtaac
     6541 tcgccgcctc ttcgggcgag aaggtgccgg cgttgctgac gccgtcggag aggaggatca
     6601 ccacccggtg ccggttcggg atggaatcgg ccatatcgac gccgagggcc agcccgtcgc
     6661 cgagagcggt ctgtccgtcc cggccgcgga tgccgtaaag cctctcgatc acccggtccc
     6721 ggtcgggaga gagataggca gcgctcgttg ccccggattc gaagacgaca atccctgcgt
     6781 agtcgtccgg gttgagccgg gagagcagga gctcagcggc ggtctttgcg gcatccaggc
     6841 gagtggggta gtaatcggtc gcctgcatgc tcccggaggt gtcgatcacc agcacgacgc
     6901 ttacgccttc gtcaacccct tcgagcggga tgtgcgggtc ggcaaggccc accaggatga
     6961 gggcgagggc gaggagggca agggcaaaga gggcgtgcgg gcgcggcagc cggcccgggg
     7021 tccggatctt tgcggcgacc gcgacccggg agaaggtgag tgccgcatac gaccgctccc
     7081 tgaccgcacg ccggtagagg aagcagagga ccgggatgcc cgcgaggccg acgagccaga
     7141 agggttctgc aaagccggtc atctaatcgc cccggtagcg cgggtgtgcg tagaaccggc
     7201 gcaggggcag gtcgtacggc ccttcggtgg agaccccgac gtcgtgcacg cggcacgagc
     7261 gcagatcgcc ctcgatacgc cggtccgccg cagccacggc atcgtcatag gcacggcgga
     7321 aggcggggtc cgaggtatcc acgaacacct gctcgccggt ctccgggtct tcgaggatcg
     7381 cgtgcccgac gtccgggagg cagtactcgg caggatcggt cacccggatg gcggtggtct
     7441 cgtgccgccg ccgcaggtgc gagagagccc ggacgaaccc cgggtcgtcg aagtcggaga
     7501 ggacgatgac cgagcacctg cgcctgaccc tcgtgcagag gaaacggaag gcggcggcaa
     7561 gatcggtgcc cgtccccgta gaccggtgcg aaatgagggc ctgcacgagc ctgatgacct
     7621 ggcttctgcc cggccgggcc gggatgaacg cctcgacgcg gtcggtgacg atgcagagcc
     7681 ccacctgccc gttctgccgg tgggcggcaa agatcaggct ggcggccacc tcgagtatcc
     7741 ggcgatcctt gctggtcgaa gacccgaacg ttgcggagcc cgaccggtcc aggacgaggt
     7801 agtaggtccg gctccgctct tcctcgaact ccctgacgac gagccggtcc atccgtgccg
     7861 atgccttcca gtcgatcgcc cgggcgtcgt cgccggggag gtactcccgg aggtttgcaa
     7921 gcgccatccc gctccccttg aaaggcgatc gcctggggcc gagacaggag ccgtcggcct
     7981 gcgcggcggt ccgaagttcg atccgccgga tctgccggat gagatctgcc cgctccccgg
     8041 cgtcgctcac cgtcagggca cctccacagc cgccaggatc tcccggacga tctcttctga
     8101 ggtgagatcg tccgcctctg cctcgaaggt gaggagcacc cggtgcctga ggaccgcagg
     8161 ggccagggcc cggatatcgt cggggacgac gtagccccgt ccggcgagca ccgcgtgcgc
     8221 cttgccgcag aggaggaggg caagcgatgc ccgcggggat gctccggagg cgatcatggg
     8281 tccgaggtcg agtccgtagg ctgcggggtg ccgggtggca tcgaccagct gcgctgcata
     8341 gcgtttcacg cccgggtcgg cgtataccga ccgcacgaac gactgaagtt cggctacgcc
     8401 ggcggcgtcg agcaccggcg ctggaggggc gggccggtcc gcagtgaacc ggtcgaggat
     8461 ggcgacctcc tcgtcgaggc tcgggtagtc catccggatc ttgagcatga accggtcgag
     8521 ctgcgcctcg gggagcgggt aggtgccctc cgattcgagc gggttctggg tggcgaggac
     8581 gaagaacggg cgcggcagcg gatacgtctc gccctggatg gtgacctgcc gctcctgcat
     8641 cgcttcgaga agcgcagact gcaccttcgg gggggcacgg ttgatctcgt cggcaaggat
     8701 gatctggtgg aagatcggcc cctgaatcgt ccggaacgcg gccgtttgcc ggtcgtagat
     8761 ccgggtcccg gtgatgtcgg caggaagaag atcaggggtg aactggaggc gggcaaaggt
     8821 gcagtcgagg cagtgcgcga gggtgctgat cgtgagcgtc tttgcgatcc ccgggacccc
     8881 ttcgaggagg gcgtggccgc cggcaagcac cgccgcgagc acctggtcga taaccgcctg
     8941 ctgtccgatg atcaccgtct ccatgccgga gcgaaccgca tcgagagaag ctgcatgctc
     9001 ccgtgcccgg ctgttgagcg ctgaaatctc cgtatccgtg cctgccatgc tggttcctgg
     9061 aaaaacgtgg gtgaggatgc tatatgacgc ttgtcagttt ggggcgagtt aattaagaca
     9121 ggttaacgac acctgctccg aatacgggcg ggctcctgcg gccgtacctc aaagttaccc
     9181 catctcatag agatctaccg attttgagga cgtcacaggt tttccggcgg tgacaggcgt
     9241 gcttatcgac agatactata cagcgcgatg ccacccgatc cggtgctgaa aatgagaggc
     9301 cgtccggtgc cctgacccgg gcttgcgatg ccatgtccca tcgaaagtac tatcctgcac
     9361 actagggaaa aacccgggca gaagaaacgg agccgtatag caagcatctg tcacgtgccg
     9421 gcagatcgtg gcgtatgcgc gatcctccac ccggcggatc attgtaatat atgaatcctt
     9481 gcctctcgaa gcagaggtta ttccaatatt tttccgatta ttcaatattg tttcatctag
     9541 atagcctgat cacaggaata tttggcacaa atagataaac acaaagtgtt tataccatga
     9601 cgacctcaat gcgaggtgtt gacagcggcc gcatgttcat ggttgttagt tgcctttttc
     9661 gtccggcttc tgtcggcagt agcccggccg tcagatgacg cggctacaat caggaaaaaa
     9721 gagttgattg gcatgcagaa caatggaaaa acaatgagat tcggtgcgat gacggcatcg
     9781 gcaattctgc tgatgcttgc cgtctgcatc gtgccggttt ccgcggacaa cgatgtcttc
     9841 gcggacaaca acgacacgtg ggctgccgtg gcgaacgatg acggagcgag attcgacata
     9901 ctcgaggagg ggacatacta tttcaacttc tcgaagccta ccggcggact gaacgcgata
     9961 cacatcaccg acagtccatc gagcctcgac ggcgccgtgt acaccaatcg gccggcatcg
    10021 ggtacactct acatttccga caccagcacg gatacccagt accatgacga cgtgatactg
    10081 ctgtttgccg tgccgtctgg tgcaacttac tcaaacctca atctactgat aaacgcgtca
    10141 gggtaccagt ggacgccgac aggagatgga agtaagccgg tacttgacgg aggctattat
    10201 cacgatccca cgctcagcga gacgttcgtg agcaccgatt tcctctccgg cacgtcggcg
    10261 tggagacccg cgcccctgga tgactatccg atatattacg gccagaacat gggcgctaac
    10321 gaccagttca agatcatgct catcgacctg tacgcgggat cggtcaacca gacaggcctt
    10381 caggacagcg ggacggtaaa ggtcgactac tacctcacag ggtacgaggg cgctgcttac
    10441 ttcgacgcct atgcatggaa caaccagtcg gctcaggcgc agggaatttc ctggacgaac
    10501 aggattatcg gcaccggcag cagcggctgg gcgatcagca tctagacaca acaaccactc
    10561 cttttcgtat gaaaatgggt cacctacata ttttcatccg gtatgcctgt ggccccacgg
    10621 gcatcatgtg gtttttctca agttccggaa gggattctcg tttcactaca cctgtcccgt
    10681 gaaataaaga ggatcaagaa tcaactctgg cgaggctccc cgaccagccc gatcctgcca
    10741 atgtgcttcg gaacccgcac gaatttttct atcagaattc agggcgatac agcagtgttg
    10801 caggaccagg gaggccctct tttctcaaga agaaacacca cgtcaactat atcccattaa
    10861 caccatttta attcaacacc ttcttataat gcattattaa aatgtaacgc attcgattga
    10921 gagattctac gcaaatgaag gcgacccgat ccccggtttt gtgtagactt cccccctacg
    10981 tccactgaat gaaaggattg gtctgacgat ggtaaattgt ggtatcacaa tcggaagatt
    11041 gcagggaggg atcatcgccg tgctgttgat cctcgtgctc ggcaccgtgc cagcggcggc
    11101 actggatgcc gacctgccgt caaacgcaac cggaccggcc gggacgccgg aggtatcccg
    11161 gatcgatgtg acgccagcag cggctgaaat gaccccgggc agcatgcaga tatgtattgc
    11221 cacagcatac gacgcatctg attcaaccat tcccggagta gcgttttcct gggcatgcag
    11281 caacggatcc gtcggaacgg tgaacgagac cggctatttc acggcgctcg cacccgggtt
    11341 cgcggtaatc agcgctggca tcccgggcag tgtggtgttc gggaccgcgg atgtaaccgt
    11401 aactacctcg atgcccgagc cgattgtgtc gatggagact gtaacggcag agacgcacac
    11461 ctggatcgtc gacgcctccg ggggagggga ctacacgacc atcggtgctg ctgtagccgc
    11521 tgcaggacca tccgacacga tcatcgtccg gaagggaagc tacacggaaa acgtcgttgt
    11581 gaatagtccg gtgaccgtcc gctcagaaag cggacccgaa gagacgatcg taatcccatc
    11641 agttcagagc actcctgtat tctccctctc cgcgaacgag tccaccgttg cagggctgac
    11701 gatccgggga tccatcggag cggcagcact cgtatccctg tcgggcgtca gcggatgcgt
    11761 tatcgaggat aacgtcatct ccacatcaaa aatcggcatc agcatggcgg atacccggga
    11821 tagcaggatt ctgaacaata cactggatac aatatccgaa gccggtatcg acctgaagag
    11881 ggccgaatcg gatctgatat cccggaatac catctcatcc tgcaagaatg gtattcgaat
    11941 ctatgatccg ggcaatcaat actcttatcg cgttaaagtc cagggcaaca cactgaccga
    12001 atgcgactat gcgatctttt ttagatatcg cacgcaagac atcgaagtca tcaacaatac
    12061 gatgacgaat ggaacgtgcg gtcttttcat tgtggacagt tcaagcgaca acactatcgt
    12121 tggaaacacc atccaacaac agtccaccta tagcggaatc ttcatgcaga atacctacgg
    12181 caatcttttc tacctcaata cagttcagga taatgccggg gatattgtgg taaacagcgc
    12241 gagcgcctcc tggaactcga cggagccgat cacctacgtc ttcgacaatg ttacccgaac
    12301 cggctacgtc ggaaattact gggaccgata tatcggatcc gatgccgacg gcgacgggat
    12361 cggcgacgaa ccatacatcg tcaaaggcac ccagaaggac tattacccca tcgtcaaacc
    12421 gagcaaccgc atcgttaccg tcatcccgga caacgtgtcc ctgacagcgg gcgacggcca
    12481 acagtttacc gccgtactgt ccgacaggga aggcacagag atcccgggcg ccgagtttgc
    12541 ctggacaagc agcgacgaaa cagtcggcac nnnnnnnnnn nnnnggaacc gcgcaggtga
    12601 ccgtccagcg aggggagacg ccgacagggg gagtcatccc tgcagtcgat gacgtccacc
    12661 ttaaggttgc aaacgatgcg ggcgtccggt tcaatgattt cggcgacaac tcgttccgca
    12721 tcctctggtc cggcggcgga ctgaatgccc tgcacatctc gaacgggtcc ggcaccgtgt
    12781 acggcgaggt aacacagacc cgtgaccggt ctggaacctt ctacatcacc acgaccggcg
    12841 gcaggggata ttttgacaat atcttcatgt gcgtggcggt gaacggtacc atcccggacg
    12901 atttccgcct gcacatccgg gccgacggat accagtggac gccgcttccg attccccgca
    12961 acgtacccac gcctgagaat cgcacctacg tgaatccgac ggtcgacgag tggttcacga
    13021 aggacgatct gtgctacggt ccgcagacct ggcgaccggc ggcaggtgcc acgtatccgc
    13081 tctatcccgg ccaggaggtc ggcgacgaat cgaacaggtt ccggatgatg ttcatcgacc
    13141 tcaaggccgg catacttccc ggctacccga tgagggtgaa ctatacgatt gaaaacctcg
    13201 agacgatggc ggccttcaac gtctacgcgt atgcccagag cgcagatgcg gtgaacgact
    13261 atgccactac ctggacaaat cgcctgacgg gagatcccgg gacgttcagc ggctggtatg
    13321 tctacggcac tccgcttccg gtcgcaggct tcacggcaaa tgtcaccgcc ggtgaagccc
    13381 cgcttgccgt gcagtttaac gacacctcga cagggaaccc gtcgtcctgg ctctggacat
    13441 tcggcgacgg caacacctcg acgggacagc acccggtcca cacctacacc ctccccggca
    13501 actacaccgt cacgctctcg gtggacggcg gcctctcgac cgccacagaa ccgggttata
    13561 tcaaagtcac cccggtccac ctcggggatg cgaacgaaga cggcaaggtc aaccaggccg
    13621 acacccttct cgtcctccag gaggtcgtgg aaattcggga gaaacccact gaccccgacc
    13681 tattccggaa gaccgacgtc caccagaatg ggatcatcga gattggtgat gccctcttca
    13741 tcgcccagcg caacgtcggc ctccgggacc cgtggtttga gcccctgtaa cccgaacccg
    13801 gtagttcgca gatcgagaaa acccaaccgt gcttctccaa cctgaagcca gcccaaaagc
    13861 aaaagatcta catgagttgc aggcgctgac gaacattgga aaacggctaa cacagcgtta
    13921 tcccggatcg gagatattta gcgtaagggg ctgaaccctt acattcttct tgccgggact
    13981 gcaatcgagg tcgatacggc atgtgagatc ggtaatctat tgaacgatag tacattaagc
    14041 actgctgcga ctttaacaaa gtcagaccgg taatcaactc tttgcaaatg actaaccgat
    14101 ctccgaatat ttttggctcg atcaattccc tacgatagat ttaataattg ctaaaaattg
    14161 ttaaccaaat tactttaata cgcccccatt tagcaatggt cttataccga acggttaaga
    14221 ttttactcat tctcagagag cggttatgct gatgtgggca ctccggatgc cggttccgca
    14281 ggagcttctt cgagaatcct tgctttaaaa ggaagatatt atgacagggg ataaacgtat
    14341 tcaaaacagt ccatggcagg ggcggatagc cgtcctgcta ctgatcctca cgctcatcgc
    14401 cgtaccggcg gtggcactgg gaaccgacct gacgtcgaac acgaccgaac cgaccgccgg
    14461 ggttctcgag gagatacctc cgaccggtac ggagggcaat accacgccgg aacaaactcc
    14521 gaccgaagcg gccgagaatg acgtgacgcc gacagcgacc gggcaacccc aggtctccgc
    14581 gcatgcctcg atgatgcata cggtcgagac cgtgcaatat gcagagacgc cgacaggtgg
    14641 cgtcattccc gcagccgatg acgtctacct ggaggttgca aacgatgcag gcgttcggtt
    14701 cgatgatttc ggcaacgaca cattccggat tctctggacg accggcggac tgaacgcttt
    14761 gcacatctca aacggggcgg gcaccgtctt cggtgaggtg acggagacca ccgaccggtc
    14821 cggaaccttc tacgtcaccg caaccggcgg caggggatac tttgacaata ttttcctgtg
    14881 cgttgcgggg aatggaacca ttcccgacga tttcaggctg catattcacg ccgacggata
    14941 ccagtgggct ccgaatccga ctcctcacct cgcacctgca cctgagaata tcagctatgc
    15001 agatgcaacg cttgacgaat ggttcacgaa ggacgacctg atctacggtc cgcagacctg
    15061 gcgaccggcg aattatgggt ctcagtatcc cctcttcccc ggccaggacg tcggcgacga
    15121 atcgaaccag ttcgggatga tgttcatcga tctgaatgcg ggtactctcg acaaccaccc
    15181 gctgagagtg aattatacga tcgagaacct cgatacgatg gtagcgttca acgtctacgg
    15241 ttattgtcag agcgcagaat cggtgaacga ctatgccact acctggacaa accgcctgac
    15301 cggggtcggg gaattcagcg gctggtatgt ctgcggcact ccgcttccgg tcgcagactt
    15361 cacggcaaac gtcaccagcg gcgacgctcc gctgaccgtt gcgttcaccg atgcctcttc
    15421 gggagacccg acctcctggg cctgggactt cgagaacgac ggcatcgtcg acagcaacga
    15481 acggaactcg actcacacct acacggcaat cggcacctac accgtgaacc tcaccgtcgc
    15541 gaacgacggc ggcagcgact ccagggtcaa gaccagctac attacggtgc gtgacggttc
    15601 ctccggcgga gatccgccca ttgccgactt cacggcaaac gtcaccgcgg gcgttgctcc
    15661 gctcacggtc gctttcaccg atgcctccac gggaaatccg acctcctggg cctgggactt
    15721 cgagaacgac ggcacagtcg acagcaccga gcagaatgcg acgtttacgt atgcctctgc
    15781 cggcacctac acggtcaacc tcacggtcga aaacgatgac ggcggtgact ctgaagtgaa
    15841 gaccgactac attaccgtca cccccccccc ggcaaagacc tggaccgtcg gtgcgagcgg
    15901 atgcgacttc gtaacgctgg aggatgctct cggcagttcg tcggtggtcg agggagacac
    15961 cgtcttcgtt tacaacggta cctatgccgt cagcggagta atcaagcccg tttcgatcac
    16021 cggtgaagga gcggagatcg taacgctgga tctgggtgga agtgatatga gggtcagcgg
    16081 gaccgggaca attatcgaga atgtgcggtt caccaacggg cggttgtatt tcaacagtgg
    16141 tggtggcgta gttgcccctc ccacggacat gattgtgaga aactgcatct ttgagaacat
    16201 gaaaaattca gttactaatt tgggagctgt ttatctaggc ctgagaaata ataccctcat
    16261 cgacaataca ttcagaaaca acgatgtggt catcctgata tacgcggcgg cgggggccgg
    16321 tggtggggac aatcgcatcg agaacaacac cttcacgaat accacctcaa cgttatccgg
    16381 taaaggagtg atccgcctgg ctaccggcaa tgggcccatc gtctgcaata attcattcag
    16441 cgacaatgat atgccctgca tagtcattat gatgaccatt aaacccggta cccggattta
    16501 tcttaacgac tttgtcgtgc cggacgggat cgctcccgta aagtggcagg gaccggcggg
    16561 tgcagccttc ccgtgggtca ccccgcagga gatcccctat acctaccagg gttcgtccta
    16621 cacgaaggtc ctgggcaact actggaatac ctacaatggt acggatgccg atggagacgg
    16681 cataggcgat acttcttact cgctggtcac gaaccagatt gatgaagctc ccctgatgag
    16741 cagcgctcag acctatctcg gacagtcgac ctccccgatc gcactcttca cggcaaacgt
    16801 caccagcggc gacactccgc tgaccgttgc gttcaccgat gcctccgccg gcagtccgac
    16861 ctcctgggcc tgggactttg agaacgacgg caccgtcgac agtaccgggc agaacccgac
    16921 tcacacctac accgcagccg gtaactacac cgtgaacctc accgtcgcaa acaacggcgg
    16981 gagcgactcc atgatcaggg ctgactacat cgacgttgcc gaaccggccg ggccttccgg
    17041 tcccgcgccc cttccggcac ccaggcacat cttcttcgaa gtcgccaacg acgccggggt
    17101 gaagtacaac ctggacggcg ctgcatacag cgggccgaac ggcacgtact acatcaaggc
    17161 ggacggcggc ggacagaagg aacttcacat cacgaacgat gcagccgtcc cggccggaca
    17221 ggttacagtc agcaacgacc ggtccggggt attctggatc accaacacgg gagggcgggg
    17281 attctacgac aacataatcc tcctggtctc ggtgaacgga acgatcccgg acgacttcgc
    17341 cctccatgtt aaatcaagcg gctatacctg gacgccttca tcgacggcga acgaattgcc
    17401 gacggaatac acctacgtcg aaggggccgt cgacgagacc ttcacgaagg aggatttcat
    17461 ctacggtccg cagacctgga aaccgggtcc gggagacctc gttgtcccat ccctgccgct
    17521 gtattacggg caggatatta acgacgtagc gacggcgtcg cacctgatgt ttgtcgacct
    17581 gaacgtaggg gatctgaaac cgggagcatt ggacgcgacc ctgaccgaga acggcggggc
    17641 gaaggtcgag ttcacgttca ccaacctgac caccacggct gcctttaacg gatacggctg
    17701 gtgcctcgcg tcgggttcgg gacaggggat ctcctggacg aacaaggtct cggacgctga
    17761 ctccagcgga tactccgtga ccggattacc gtactcaccg ccctctgcac cgattgcgaa
    17821 cttcacagct aacgtcacca gcggtaacgc accgctgacg gtccagttca ccgatgcctc
    17881 aaccggcagt ccgacctcct gggcctggga cttcgagaac gatggcacaa tcgacagcac
    17941 cgagcagaac ccggtccaca cctacaccac agttggcacc tacacggtca acctgacggt
    18001 cgcaaacgac ggcgggagcg attccgccgt gaagaccgac tacattgttg ttacggagtc
    18061 gcagaacccg cagcccgccg gcatcgtcgt caccccggcg acggcggaac tcaccgtcgg
    18121 ggcaacgcag gcattcgccg cgaccgtcta cgacaccgac gggggcgcga tggatgacgt
    18181 caccgtcacc tggacgagtt cgaacgggac cgtcggcacc ctgaacaccg ccggcctctt
    18241 caccgcccgt gccccgggca cgacgaccgt caccgccgcg tacgccaacg tagcgggcac
    18301 cgccgtcgtc accgtgaccc ggccgcaggg cgaccggacc cgggacaccc ccctggacat
    18361 ccccggctgc aacgtgacca cgggcaacga cggcaagccg caggtcagca tcaacaccac
    18421 cgcaaccaac gcaaccgtct ccgggaacac catccggatc gttgagggca acttcaccct
    18481 caccatcgag accgagggcg ctcccaccgt cgggaacggc accgtgaacg gaacggtcgc
    18541 cgggatcacg ctcgacacgc agccggtcgt cacggaactc ggttcggtcg gcacggtcag
    18601 cgcctccgtc tcggcgaacc tgaccggcct cccgcagggg gccggcctca cgacgaccgt
    18661 ctcgcagaac gtctccgccg atgcacagag cgccttccag ctcgccgcca gcgccgacgg
    18721 gctgaccctc ggcgatgtcg cctacacgat gaacatcgtg aggacgaacc tcgagaacgg
    18781 ccaggacatc gccggcgcca ccatccggat gaccgtcagc cccgcctggg tcgccgccca
    18841 cggcggcgcg gacgcaatcc ggatcatccg gtcggccgag gacggcacca gggaagtgct
    18901 cgcaacgacg ctcgtcggca ccgacaccga cgggatgatg gtctttgagg ctgtctcccc
    18961 gaacggcctc tcggtcttcg ggctggccgc agccacggca gcatcgtcgc cctcatccgc
    19021 ctcctcctcg tccggcggca gcagcggcac ctcgaccgcg gtcggtgcgg caagcaacct
    19081 caagtccggc gagagcgtca ccctcgcgat ggacgcgacg gcgatctcgg cggttaccct
    19141 caccgccaag accgacatca aggacgctat ggtgaccgtc gcaaagggct cactcccgca
    19201 ggccgccgaa cccccggcgg gcacggtcta ccagtacgtc caggccaccc tctacaaggc
    19261 gtctgaagcg gacctgagtg ccgtccagtt ccggttcgcc gtcccgaaca gatggctcgc
    19321 ggagaacggc tgcaccgcag gagaggtcgt gctgctccgg tacgccgacg gcgcatggca
    19381 ggcagtcccg gtcgaggtcc tcggcgagga gaacggcaat gccgtcttct ccgccgatcc
    19441 tgacgggttc ggcctctttg cgatcatcgc gaacgagcag gctcccggca cgaccggcga
    19501 ggcgacgctg cagcccactg caacggcgac accggtccag gcggaagcag cggcccagac
    19561 ggccggaagc acgcccacgc cgcaggcgtc cccgctcccg gtctgggcgg caatcctggc
    19621 attcggagtc ctcttcctgg tgaagaggag aacctgatta cccttttttc cgatgatgcc
    19681 cggggaaagg ccagaatgca aacactctac gcaaacgccc ggccgggatg gtcggagact
    19741 tacaggagaa gaatgcaatg aaaacaggaa agagacaccc ggccgggtcg gggggtacga
    19801 ttgtcgccct tgttctcctc gtcgcgctca tcgccgcacc ggccgcggca gccgacatcg
    19861 gagggatcac cctcccggat tacaggcata tcgacatcac gatggcgaac ggtggagaga
    19921 agtatgtcaa gttcgacggc ggcggtctca acgccctcca tatcacgacc gacccgtccg
    19981 aacgctacgg acaggtgacg gagaccagca cccaatcggg caccttctac ttctccgaca
    20041 caggcgggag gggcttcttt gacgatctca tcctgatggt ggcggtacag ggagaggttc
    20101 ccgacgactt caaggtccac gtcagggcaa gcggctaccg gtggacgccg accgccgagt
    20161 tgaaccggcc cccgacgaca gacgagatcg agcatgccga gggcgccctc gaccggatct
    20221 tcaccaggga agactttatc tacgcgccgc agacctggaa gccctgccgg gaggcgaatt
    20281 acccgatcta ctacgggcaa gacatgagca ggaacaatcc cgaagaccgg ttccacatca
    20341 tgttcatcga cctcggcgtc ggcgcgctcg gcccgaacac attccttgac gggctgaccg
    20401 acaacggatg cacgaagatc gagtactcct tcgagaacct ggagacgttc gcggccttca
    20461 acgcgtacgg ctggtgcaac cagtcgaacc aggggcaggg gatctcatgg tccaatgacc
    20521 tcacaggatt ctccgaaagc ggcgtgagcg gctactcggt caagggcacc aggaaaggcg
    20581 gcgatacggg tgggtccggc ggcggaggca gcagcagcgg aagtagcgcc ggttcatccg
    20641 gggggtcggg catcaggggc ggcacgctga atgcatcgac gacgggctcc atcaacggca
    20701 ccctccacgc cttcaccgca tccgggccga cagaaccgtt aacagtcggg tctccgtcga
    20761 ccatacggat tcccgtcgcc gtccccgaag gcgccacggt cgaccgcggc ctcctctacg
    20821 tctacgtgag caacgcaacg gaggcgaaga caggcgccgg aaccgagttc gagccggttt
    20881 tcaggtttga cggcaagccg gtcacgcccg cggccgcatc gtgggaccgg cagggaaccg
    20941 gaacggcttc gatggccggg accttcaccc tggagctccc gccggagatg ctcgccaacg
    21001 gcga
//
LOCUS       DAJB01000227            6138 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_74209, whole genome shotgun sequence.
ACCESSION   DAJB01000227 DAJB01000000
VERSION     DAJB01000227.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_74209"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1158 a         1836 c         1856 g         1288 t
ORIGIN      
        1 ctccttgccc gtcgcatgtg aactaccccc cgcttgcgca gggggcttcc tgcgagtgtc
       61 cgggggtcac agggatcctc agacctgttt gtcgcagatt accagtgaac tcccggtagc
      121 ccgtccacag ggcattctgt gataggtatc ggaccataat gttgatcgcg gcgttcctgt
      181 cgcggtcgag cacggtacca cactcacagt gcataacacg atcccgaact gtcatctctg
      241 cgtggatctt cccgcagcaa cagcacgtct tgctcgtgta ctcttcgccg acctctatta
      301 cctgtttacc tgcaaggatc cccttgtagg tcagaaatcc ggcaaaccgc gcaaggtgtc
      361 cggttccttg cgtagacctg tttgtctgtc tcgttgccga cctggacttc ggcatcttct
      421 tcacggaaag atccccgatg acgatcgttc cggcccgggt gttctcaacg aggttcttgc
      481 tcaccttgtg ctggaagtct ttgatctggt cgcttcgctt ccgttccatc ttccgtttgg
      541 tagtattgta gctgatccac cgcctgctgg gttttttacc cttgaccttc ttgcagtggt
      601 cccttcggga ctgcacggca tcgatcttcg ggttccagta ccgatccggc cggatgttct
      661 tgacctcaat gaacttgccc tggctgttga ccgccgtctg cttgatcact cccagatccc
      721 aggcctgata ctgtccgtta tcgacggggt tcggagggag gacgtcttcg accacagcga
      781 ggaaccactt ttctttgctg tcccgaaaca cctcaacctg cttgatcgcc ccgaactctt
      841 gctcgggaat cgggaaggtc aactctacct tgccggggag gttgtgtgac aaccggatcg
      901 aagtgttggt gaccttgaac ccggactggt tgtgcttcat ggtggtgaag tagtgtcgcc
      961 ccttgaagcc cggggggttg gcgtctacgt caccgttctt tcggagctcg aagaacgact
     1021 tgtagttggc gtcaagggtc ttgagggtca tctgcagaac cttggcgttg acggttttga
     1081 gttccggcca ctctttcttc aggacaggaa gatcgttctg ctgtttgatg tagttgaccc
     1141 ccttgatccc ggcagcatgg gcttcccggc gttcgttgag ggccaggttg tacaccttcc
     1201 ggttgatctc ggataactcc cacaggacgg cttcctgccc cgcggtcgga tagattctaa
     1261 tcttccgggt caactgcata ccgatcctcc gcctgacgat ctatatactt cttcaggata
     1321 tcgagcgtta cctgcccggt cgtggcaagg aaatacgatc tcgaccagaa cgcatcacgc
     1381 cagagcacct ggttgacttc cgggaagttc cgccggatct cccgggaggt aatggtcttc
     1441 agggcgttga tgtacttcgg gagatccaga gtggggctag ctttgaacag catgtggaag
     1501 tggtccatgc tgcactcgat gttggtgaca tcaaccccga aggtttcgga tatctcatgg
     1561 attttggtct tcaggagatc cacataccgg tcatcgacaa agacgttcct ccggtacttg
     1621 acgcactgca ccaggtggta gtgcaaggcg tagaccgaat gagcaccttt gtccaactgg
     1681 tatgtcatag ttacttttta gtgggagtaa ctataaaggt ttctgttggc acgagggcgg
     1741 ctctcatccc ccagcttgcg cagggggact tctcgcctgt tgtgttaaat agcgcagtga
     1801 cccgcctcgc tgccctgacc agggaggcac gcgatgatgg cacgggaatg caccatcagg
     1861 acggagattt cgggacatct ccggaagcaa ggaacataaa ggtttttgcg aggcaaacga
     1921 caatatgagt acaatgctca ggataaaccg agacaagtgt ggatactgcg gcacctgcgt
     1981 cgcagtctgt cccgaggacg cgctcgagct gatcgacgct tacctcagcc ttgagcggga
     2041 gtgcatcgcc tgcgggatct gcgcgcgggc gtgcccgctt ggtgcactgg aggtcgtcca
     2101 tgaagagtaa gtatgatgtt ctcgtcatcg gcggcgggcc cgccggcgct cttgccgcga
     2161 aaacagcggc acaagccgga aactcggtct gtctcgtcga gaagcgtcct gccatcggca
     2221 cgccggtccg gtgcgcggag gggatcggca aagaactctt aaaggaatac atcaagcccg
     2281 acccccgctg gatatccgcc gatatcgaga gtgcccggat catcgccccg aacggcaccg
     2341 ccatcagcct cgaccaggcc cgggccggaa acgaggtagg ctacgtcctc gaccggaaga
     2401 tctttgaccg ggaactcgtc tggcaggccg ccgaggccgg ggcggacgtg atcgtcaaga
     2461 cccgggcgac cgcgccgatc atcgagaacg gcgcggtccg gggcgcgaag gtgctctctg
     2521 ccggcgtccc ggcagatatc cgggccgaag tggtcatcac cgccgacggc acggaggcac
     2581 agttcgcccg gtgggccggg ctcgacacca ccgtccccct ccgggagatg atgagctgcg
     2641 cccagtacct gatgacggat atcgatatca acgcgggctc gacggacttc tacctgggca
     2701 acagcatcgc gcccgaaggc tatctctggg tcttcccgaa gggcgatcgg acggcaaacg
     2761 tcggggtcgg catcaccggg aggaagagcc gcgacggatc ccgggcaaag gactacctgg
     2821 accggttcgt cgcaaagaac ttcccgaatg ggaaggcgat tgaagcaatc gcgggtgggg
     2881 ttcctgtctg ccggccgctc ccctgtacgg tcagcgacgg ccttctcgtc gctggcgacg
     2941 cggcgagggt cgtcgacccc atcaccggcg gcgggatagg gaacgccatg tataccggaa
     3001 ggctcgccgg cgaggtggcc tcaaaatgca tcgaggcggg caactgctca aaggaggccc
     3061 tgatgcccta cgacacggag tggcgtgcat ccagaatggg catgacgaac gagcggaact
     3121 acagggtcaa agagtacttc atcacgctcg acgataagaa actcaacacc ctcgcggact
     3181 cgattgcaag catcagtttc agtgagttat cggtctcggc actcatcagg gaactgatca
     3241 agcggaaccc gaagatgctg ctcgagctca aggcgctcaa agatacccta gtctgaccgt
     3301 atcaactgtt tgttgagggt ctttatcgtc tccccttccg cttccttttt tgtaaagacc
     3361 gtgatgatat caccgggccg gatacggacg gtgccgctcg ggatgatcag gtcgcccccg
     3421 gcacgccgga tggcgatgaa gacgaagtcc cggacatcga ggtcccggat ctcatggtcg
     3481 acgataggcg cgccctcatc ggcaacgatc tcgaagatgg tgccgccggg gatcgaggcg
     3541 acctgctgga ggttcgggct cttcgcccag tagtagagcc gggtcgccac cagctcgtcg
     3601 gggttctcac tgatcttcac tccgacctcg ttaaataggt cggaatgctc cttctgattg
     3661 acgatcgaga cgacgtttcc cacgttgtag cgtttcgcga gccagcaggt catcaggttt
     3721 acggcgtcgt cgctcgtggt agcgaccagg gcgtcggccc ggtcgatccc ggcatcctgg
     3781 agcaccgatt tgtcggtcgc gttcccggtg atggcaagaa cgtcgtaatg ctccagaata
     3841 tcgctcgcac gttcctcatc ctggtcgatc accactacgg agtcaccggc gttcacggcg
     3901 atcgcagtca aatttcgccc gattccgccc aggccgacga tgatcgtgta catccatctg
     3961 gattcaatcg tcagcattga aataccttgc gaataaacca gaagatgtga tctgcggggt
     4021 ggatgaagcc gggaagggct cagttctcgg gccgatggta gtcgccgccg tcgggtgccg
     4081 ggcgactgag gaccttgcgg cgctccccat ccgggactcg aaggtcctcc ggccgaaaca
     4141 gcgcgaggcg ctttatcaga tcctcttccg tgacttctcg atagcgatcg tcaccatcga
     4201 tgccgccggg atcgacgatg cgcggagcag gatgaccatg aacacatgcg tggccgagct
     4261 ccatgccgag gcgctgacgg ggctccggcc ggaatctgcc tacgtggacg cctgcgacgt
     4321 gaacgcggag cgctacggcc ggacggtcgc ggcctacctc gatttcccct gcgagatcgt
     4381 cgccgagcac cgggcagacg cccgccacaa gatcgtcggt gcggcgagcg tcgtcgcgaa
     4441 ggtcacccgg gaccgggcca tccaggaact cagtgagcag tacgggaaga tcgggagcgg
     4501 ttacccctcc gacccgacaa ccatcgagtt cctccggggc tacatcaggg agtacgggga
     4561 cccgccgccc tgcgcccgcc ggagctggaa gacggtgacg aacctttacc agacgacgat
     4621 gcgggatttc tcataaccag gaagatttta tgcctcccgc acgccaaccg tgagtgatga
     4681 gctggctcca gatactcctt cttctcgtcg cggcctacgc gctgatagcc ttttacatcc
     4741 gtgaccgggg gctgtttcgc gatcacgtca tgttcttcgg ccccatcctc gctatcaaga
     4801 ccgagcgggt cggtttcttc gactggttcc ggaagttcaa gacagtgctc cgggcctacg
     4861 cgacgctcgg ggtcgtcatg gtcgtggtgg tctcggcctt catgacgctg gccctgatcc
     4921 tcgcggtccc ccagtatctg gtgcatactc ctgaaccgac cgggatctac gacccccgga
     4981 atatactcgc gatacccggg gtcaaccagg cgataccgat caccgcggcc gtcatcatcg
     5041 gccttctgct cacgatcgtc gtccacgagt tcggtcacgc gatcctcgcc cgggttgagg
     5101 atatgcgggt gcggagcatg ggtcttctca tcgccgtcgt cccgataggg gcatttgtcg
     5161 agcctgacga ggaagacgtg gatgcggcaa aggggatgcc caagattcgg atgttcggcg
     5221 ccggtatcac gaacaacatc gtcatcggtc tcctctgttt cgtcgtgatg ttccttcttg
     5281 tggggatggc gacgccactt tccgtcccgc tcgtccaggg ggtctaccag gactttcctg
     5341 cggccgaggc cgggatcccc ggctactcgg tcatcacggg agtgaacggc gtcccggtga
     5401 cgagccagaa ggagatatcg gcgatcatgg acgggacgcg gccgggggag acgatcacgc
     5461 tgacggctgc agaagacggg gttgaaagca cgtacacgct caccctctct gagtggccgg
     5521 aaaccctcgc cgacgaccgg gactccgggt tcatgggggt ctactactac aacgcgcccg
     5581 cggtgaagga acatatcggg aacatcgcgg atctggggcc gctcgcgccg ctctacctga
     5641 cgatagcgcc gatcgacgcc ttcatccagg gcaacaccca gcagctcgcg atcctgctcg
     5701 ccgacacccc cgagcagata gcctgggagg agccgttccc cttcttctgg ggcacggtcc
     5761 acctcttctt ctggaccggg tggttcaacc tgctggtcgg gatgttcaac gccatcccga
     5821 tagtcccgct cgacggcggc tacatgatga aagaaggggt ggagcggttc tttgagcggc
     5881 ggggctggtc ccggtacgcg cagcgggtcg tcgcctcgat cagcgggttt gtcacggtga
     5941 tgctgatcct cctcatcacg atgccgatga tcgtcgcggc ggtgcggttc gtgctgacga
     6001 tagggtaggc caaatatctt tttcaagtgg aggcgcggcc acctaaactc tcgcgcgcct
     6061 ctaccatcgt caggattccg gcgacaatat cattgcgact gaccggtcgt tcacgacggg
     6121 gcagggccgg agttacac
//
LOCUS       DAJB01000228            2640 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_118850, whole genome shotgun sequence.
ACCESSION   DAJB01000228 DAJB01000000
VERSION     DAJB01000228.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_118850"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          531 a          828 c          814 g          467 t
ORIGIN      
        1 agtatgctat tcaaggacat taccaaaatt cctaacataa agtgacagga tgcactagag
       61 gtgcccgagt tctttcaggc tcacgtaacc cttcttcgtg acgatgacgt ggtcgagcac
      121 ccggatgccg aggatggacc cggcctcgac gagttgccgg gtgatgctga gatcctgggt
      181 ggagggctcc agggtgcctg acgggtggtt gtggaccaga acgaccgagg cggcgcggtc
      241 ggtgatcgcc tcggcaaaga cctcccgggg gtggacgagg ctctggttca ggatcccgac
      301 ggtgacggtc cggcgctcga tcacctcgcc ggctccattg agggttatgc agaagaagta
      361 ttcctgcttc ttatcccgcc attccgcaac aagcgggagg acatcggcgg gacaggtgat
      421 ccggtgtccg gagacgccgt cgtccccgag atatcgccgg ccgagttcga aacaggccat
      481 gatctcgcag gctttcgctg acccgacccc atctatcgca aggagggcgt catacgatgg
      541 gccggccccg gcaccctcca ggtagcgttc aacatcgctg gcgacctgcc agacgtcccg
      601 tgtctttgtg ccccgcccga ggaggagagc aatcagctcg gcatcggtga gcgcctgcgg
      661 tccccgtgtt gccagttttt cacgcggacg atcgcggtct ggtgtatcgc gcatcctctt
      721 catgctcatc cggcccgggt gggttaccct gatcggcggt tttgatcccg gagcatgtat
      781 acgttgtcaa ttgatcccct gcatcagacg ggtttaccgt ccgaaccgtc ttccccggtt
      841 cggaaaagtt cccttctgac cggggaaatc ccgggagcgt tatcgaaatc ctttttgcca
      901 gagggttgcc atctctcaca aaatggaact cccctggaag agcgccgagg cctggtgcag
      961 gaagggacgg gcatacgtcg agcaggggca gtacgaccgg gctgtcgagt gctacgaccg
     1021 ggcaatcacc ctggatgcca acaccgccgc cgtctggtac cacaagggac tcgccctcac
     1081 gaccgcccga aaacaccggg aagcggccga atgcttcgac caggccatca ggatcgaccc
     1141 aacctgcgcc cggctctggc atgcccgggg gcaggcgctc tatgacctcc aggagtaccg
     1201 cgaggcggcc gattactgcg gccaggcggt gaaactcgcg ccggagatcg cgggcgcctg
     1261 gcttatccgg ggccatgccc tcaggaagat agggcgaact cccgacgcga tcacctgcta
     1321 cgaccatgtc gtcacgatcg aaccggggcg cgtcgaggcc tggctcgccc gcggaacggc
     1381 gcttgctgcc gatcgctggt acgacgcagc ggtcgactgt tacgaccggg tcgtcgcact
     1441 cgatccccgg aacgcaaatg cctggtacgc cagaggaacg atcctgatcc tcctctcccg
     1501 ctacgaggac gcgctcgcct cgtatgatcg ggccgttacc atcgatccga accacgcaga
     1561 cacctggtat acccggggat gcacgcttgc agcgctgaag cgctatgatg aagccatcgg
     1621 atgcttcgat cgggcgctcg ctcttcgtcc cgacgacgcc gaggcctggt acaaccgggg
     1681 tcgggcactg cagaacctgg aacgctacga ggagacactt gtctgctatg accgggcgtt
     1741 ccgggtcaat cccggccacc ccgggatctg gtaccacaaa gcgatagcac tgaagaaact
     1801 gaagcgttac gacctctcgc ttggctgctt cgaccgggcg ctcagggaga acgccgtcga
     1861 tgcggagatc tggcaccaga agggacttct cttctttgtc ctgaaacgct acggggaggc
     1921 gatagagtgt ttcgatcagg cactcagggt ccagaccgac cacaaggacg catgctacta
     1981 ccggggcgag agttactacg ctctcgagga ctgggaggcc gcagttgcct gctaccagac
     2041 tgtggtcagg gcggccccca cgaatgccgt cgcctggaac aactacggaa acgcgctcta
     2101 tcggcttgaa agatacgagg aggcacttgt ctgctacgag cgggcgctcg agatcgaccc
     2161 ggagaaccgc caggtctgga gcaacaaagc aagcgtcctc tcagttctct cgcactacga
     2221 caaggcgctc gtctgctacg accaggaact ccgggcccac ccggagaacg cggacgcctg
     2281 gtacaataag ggccttgcac tcttcatcct cgggcggtac ggcgaagccg tcacctgcta
     2341 cacccatgcg cttgagatcg acccggagag atccggcgtc tggggcacga aggggaatgc
     2401 tctcgtcatc ctggaacgct gcgaggaagc ccttgactgc tacgaccggg caatcgctct
     2461 caaccccgac gacgccgacg cgctcaacgg gaaggcgatg gcgctcgtct atctcgaccg
     2521 ccccgccgaa gcggtcaaat actacgagag ggtgcagcgg ctgccggagc gggcctgggc
     2581 tgcaaaaccg aattcgagta aaaataagag gatctagacc cgcacaatcc gcctgaccgc
//
LOCUS       DAJB01000229            2713 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_129448, whole genome shotgun sequence.
ACCESSION   DAJB01000229 DAJB01000000
VERSION     DAJB01000229.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_129448"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          671 a          735 c          751 g          556 t
ORIGIN      
        1 ctaatcgcta cgggaggtta ggttacagct attctttatg cccaggagag cccaatatgc
       61 ctgtagagcc gggtgtgccc cacccggctc tacgcttgcg gagatcggga ccgctacacc
      121 gccgaccctg gcggtgcacg tcccggtcaa cgaaccagga atctcccgga ctttagcccg
      181 gggagagtgt caaggagtgt atccagatgt tcggagctcg actgtgagta gatcgtcccg
      241 gtttcgaata gccatccccc ctcgggtggc ggccgggccg ttaggagaag gcgtttctgc
      301 caccctgacg gatatgaacg aggagtgcat catgaaccgc catagaagat ccggggatca
      361 cagaagagaa agcccggcgt tcggcagggt ctccacaata tcgtacgagc aggggtggaa
      421 ctatgagcgg agatgagaag aaactgatac ggaacagcac ggttgaattt ttgctattta
      481 cctcggccgc cggtgaagac tcaatcgagg tcaggtatga ggacgaaacc atctggttaa
      541 gccagaagat gatggcggcg ctcttcgatg tgactgttcc gacaataaac gtacacctga
      601 aaaacatcta cgaaagcggc gagttgaccc gtgatgcaac tattagaaaa tatctaacag
      661 ttcaaatcga gggtgatcgt gaagtttcac ggactcttga ctactacaac cttgatgcca
      721 taatctcggt tgggtatcgc gtcaattccc gaagagccac ccagttccgt cagtgggcga
      781 ctcaggtgtt aacagaattt gccattaaag gatatgtact tgataaaaag cggatggaga
      841 acggtacgtt ccttggggaa gactacttcg agcgcttgct ggcggaaatt cgggagatac
      901 ggctgagtga gagacgattc taccagaaga tcacggacat atacgctaca agcctcgact
      961 acaacaggaa tgcaccaaca acacgcgagt ttttcgcaaa cgttcagaac aaattacatt
     1021 ttgcgataca tgggaatact gcggctgaac tgataatggc gcgtgccgac agcagaaaga
     1081 agcatatggg ccttagcacc tgggaaaaag cccctgacgg gaagatactc aaaaccgacg
     1141 tatcggttgc gaaaaactat ctgaacgcaa cagaacttga atcgctcgga agaatcgtca
     1201 acgcctggct tgaccttgct gaagagcgtg ccagaagaaa gatcccgatg actatggagg
     1261 attgggcaaa acgccttgac cggttccttg agttcgatga gcgcaaactg ctcgaagacc
     1321 gtggaaaggc cacaaaagag atggcaaagg agttcgcaga gagtgaattt gagaagtacc
     1381 gcgttgttca ggacagactt tttgagagcg atttcgaccg gatgctaaag gaggccgacc
     1441 cgcccgaagg agaggaacgg gataacaaaa acaatgagac tactggagac ctgaaataaa
     1501 ctactccgtg gtgggcggca ccagacggga ggaatacctg atggggtgta ttcggagtgc
     1561 ccgcttcctg atatcaatgt cggaaatatt gccgacgatc tgcatatgtc cagatatctg
     1621 ccggcgtttc cgtacttgat gggtacttgc ggaggggcca gaggagcatc acatccagca
     1681 ttatcaccat agacagaaca gtcttcgaca cttcctgcca gggggttcca ggaggaccag
     1741 ccgatcagga gtcgttgatg ccccctcaca cggctcatgc tccacgaccc gatctccggc
     1801 acccatcgtt gccgctgcac ccttcgcact ccggcatccg gaacagctgc cgctcgatgc
     1861 cggaaaccgc atcacgaatc tcaccaggaa ctctctgtcc gtctaccgcg acctccaccc
     1921 agcggtcgct atcgacaaaa tagagaaaag gtttgactgc ctcacccaga atatgctcgg
     1981 cagcaagccg atagatggtt atctggactg cgtaatcctt gactttttcc gggacatggt
     2041 ccacatcagc agcacctgtc ttatagtcga tgagcaccca ggtgccgtcc ggccgccgaa
     2101 cgagtcggtc gatggcgcct ttgaacttca caccaccgat gcttgtccgg aacggcacct
     2161 cgcagtagtc gttcgtaacc ccctgcatca cctctgccgc acggaagcgg tcatagagta
     2221 tctggtattc acccacgatg ctatcatcct caaccccgta gcgccgcagc acggctccgg
     2281 ggtctctgcc ccggaagacc tcgtggacga tcagcccccg cgtttttgca tccattgccc
     2341 cggatgcctc acggatcggc tgcgtcgggt tgttaagccg gaatttgcgc tcgtatgcga
     2401 gcgggcagcg gaggtactgc cggatctcgc tcgcggagta cacgtgctcc tcttcatcga
     2461 cctggatggg aggaatacct tccgacaccc cggccccatc gctcggcagg gaaaggtgta
     2521 tgagcccgat ttgctgcggt tctgcgcata ttgctgcagg atctgtaacg agagagatgc
     2581 aaagcgggga ctcttcacca ggaatggtga tctcggccgc tcctcgtatg cagacatcat
     2641 cgcagagccc gacacagtgg gcgagccagc ctatccggtt cttcgccccc tccagggttt
     2701 tcgggacctc gat
//
LOCUS       DAJB01000230           11946 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_16400, whole genome shotgun sequence.
ACCESSION   DAJB01000230 DAJB01000000
VERSION     DAJB01000230.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11946)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_16400"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    971..1082
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2236 a         3606 c         3803 g         2189 t
ORIGIN      
        1 ccccgagaat gatgatgtat ttcatgcgaa ccttctttgt gatctcgatg atgaacggcc
       61 tgctgtaatt cccttgaaag tgtatcgctt ctggccccgg gagagtgggc gcgcgcctct
      121 acccgagcat ggcacggacc gccgtgcgca cgctctcttc cgaggcggtc ccgcaccgcc
      181 agccgagatt ctcgatcttt ttaaccgaga gttgcatcct cggcacatcg ccgacccagc
      241 cgcgtgcacc gccggtgaac cggtagcgga caccggagag acccatctct tctgcgacga
      301 tgtcggcgat ggcgacgacg tcaatccagt cttcagagcc gatattgaag gtgttcaccg
      361 ggtcgtgcga gtgttcgatc ccgaactgca cggcgcggac gcactcccca acctccaggt
      421 acgattttgt ctgcctgccg tcgccgagga tctcgagttc gtgcgggttc tcacggagtt
      481 tccggatgaa gtcggagatg acgccgtggc cgctccgctc gccgatgatg tttgcgaacc
      541 ggtagaccca ggacgtcatc tcaaacgagt ggcagtacga cgatatcagc gcctcgcagg
      601 cgagtttcgt cgcaccgtag acggatatcg gctcgagcgg agtataggtc tccggcgtcg
      661 ggatgacggc cgcatcccca tagacggtcg aggtcgaggt gaagacgagt tccggcacac
      721 cgtgcgcccg catcgcctca agaacccggt gcgtgacgac gatgttgttc cttatctgtg
      781 agtccggggt cacggcgctc tgccggacat cggggtctgc ggcgatgtgg tagacccgat
      841 cggcgccctt gaagtgctcc tgccagccgt catcgagcag gttctgcctg atgaaggtga
      901 cctgcccgct cgccgtatgc tgagcgaggt tcctttcagt gcccgcactg cagtcatcga
      961 tgaccagcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnagagatcg gtgatgcggc agcacgggga aaggttaatg cccgcatatg gtttatttct
     1141 cgatccggta gtttactccg gggcatattt tacctgatta cctccaacat atgaagaatc
     1201 aagaggagtt gtctaattga gagaggcatg catccgttgc aggaccggcc ggtctttgat
     1261 cgccgctgca aaacagaccg tgcaggaatc ctttgcaccc acgcaggtgt ccccgcggcc
     1321 cggcggcgat agagccccca tacagacccg ggcatccggg cacgaccaca ccggcgccgg
     1381 catcaccccg gcaaccgtgt tcatcgatac ctgctccgga gccgggccat gagcccgggc
     1441 ctccgcgaac acgccgggat ctacctgcgg ggtctgatga tgggagcctg cgacatcgtc
     1501 cccggcgtct cgggggggac gatcgccctc atcaccggga tctacgaacg gctggtcggt
     1561 gctatcggca gcgtcgaccc ggcttcggca aaacacctcg cgaagggcga cctggagtct
     1621 ttccgggcag acctcgagag gatcgatctc ccattcctcg tcgtcctcat tgccgggatc
     1681 ggtactgcct tccttctgat gtcgcgggtg atcctctccc tcctcgccaa ccacgcggta
     1741 gagacctact ccttcttcct cggcctcatc ctcgcctctg cagtcgccat attccttgag
     1801 atccgctccc cgggcgcggc cacccttggg tacctcgccg tcgggaccgg cgccgggttc
     1861 ctcatcggag ggctcgggca cctcgacgtc ggccactctc tgccggtcct cttcctcacc
     1921 gggatggcgg cgctctgtgc catgatcctc cccgggatct cgggggccta cataaccctc
     1981 gtcctcaacc agtacgagtt catgcttgcg gcgctccggg ccctctccct ccccgagatc
     2041 ttcgcctatg ttgccggcgg tgttgcgggc ctcctcctct tcacgaaggc cctcaagtac
     2101 ctcttaaaga cctaccacgg ggcgatgctc gccctgctca ccgggctgat gctcggctcg
     2161 gcgaggatgc tctgggaaaa aggcgccgca gccggggata tggttgccgg cggctgggtc
     2221 ttcttccttg ccggcctcat cgtcgtcggc gcggtagagt gcgcgaagag gcgctatcag
     2281 ggcagcgggg cctgatcccc cctccccttc gagacgatcc gaaaacttac ttccacccga
     2341 aggtgcaacg agatatacat catgtttatc ggcatcgacc acggcactac cgcgatgcgc
     2401 ttctccaccg gtgaagcgga gttcaagatc tcccgcgaag acgccaggca cttctcggcc
     2461 ggcgatcttg cccgcctctc ccctctcgac gagatcgagg gtatcgccgt ctgctactcg
     2521 atgggcgacg gcatctcggc catcaccgat atcgggaagg ttgaaggccg gggcgtcatc
     2581 tcccgggagg gtgccggcaa gcacatcggc ggcggcaccc gggtctacga cgagatcaga
     2641 gagagcggtc ttcccgccgt cgtcatcccc ggattgcacc gtgcatcacc gaccgatccg
     2701 cggttcaagg cctactccca ccaggcgagc cccgagaaga tcgggatcct gtatgaggtc
     2761 tcccatgacc tcgggggcga catcgtggtc tgtgacgcga gctcgaacac cgtcaccctc
     2821 ctcgtgaccg gcggccggat caccggtgca tttgatgcct gcatcttcgc acccggcacc
     2881 cggcatggtg ctctcgatgt ggatgccatc cgccggatcg accggggcga atccacggcg
     2941 aacgatgcct tcctccacgc gggcgtggac cactcgttgc ctccggaact caggatagag
     3001 acgatggcca tgtttgccgc gatggaatgc gcctccatgc ttctgataaa tcccggcgca
     3061 aaggtcgccc tcgccggctc catggcacct gccattgcac cgggggtgga ggtgctcctc
     3121 tcgcgggagg tcgccgtcta cgacgagtgg tgtgcgtccc ggggactcgc caggatcgct
     3181 cgcgatgtct tctcgggagc gaccgggatc ctcgggctcg cggtggagcg gtagcccccg
     3241 cccgggcgcc cttcccattt cgtactcttt tatctggtgc cgggtagtat agtggactaa
     3301 agaggagtga ccagaagcgt gcagccggaa tgccccagta tcgctgatct catgctcctg
     3361 atggcgtcaa gtctccagga gacggcacgc gccataaatc cggagaatgc aggtcagttt
     3421 ctcgatgaga tcctcaacgc gaaacggatc taccttgccg gcgccggacg ctcgggcctt
     3481 gttgccagag cattcgccca gcggctgatg catcttgggt ttgagagtta cgtcatcggt
     3541 gagaccatca cgcccgcgtt cgggccggga gacgtgctcg ttgccttctc gggttcggga
     3601 gagacccagt ccgtcgtgga cgtctgtgag accgcccggg agatcggggg gaagatctgc
     3661 ctcatcacat caacacccaa ctcgcatatc gggcgcatgg ccgactgcat tgttgagatc
     3721 gggaataacc cgcacaagga cccggatatc ccgcgtgact atgagatccg ccagctcacc
     3781 gggcagtaca ggtcggtctc cggatccttc gcccctctcg ggacactctt tgagaccgca
     3841 gcgctggtgt tttccgacgc gatcgtctcc gccctgatgg aagtgaagca ctgcacgatc
     3901 gaggagatca agggccgcct cgccaacgtt cagtgagcgg ggtatcccgg caacccctgt
     3961 tcgaactctt tgtagccacg ccgggcgatt gtatttaaga cgtctgcggg gcaatgggtg
     4021 ttccggcacg ctcgcggctc cggggggtct tgccgtgtgc cggagtattt atattattaa
     4081 tcattaagat ttaaaggggg tttttgtttg agagagttac gcattcatgg caggggtggt
     4141 cagggttccg tgaccgccgc cgaactcatt gctttcgcag catttgaggg cggtgtgttt
     4201 tcccaggcat tcccggcttt cggggtggaa cggcggggtg ccccggttca ggcgttcgtc
     4261 cgtttcagtg acgagaagat ccggctgcgc agccaggtgt atgagcccga ctacatcatc
     4321 gtgcaggacc cgaccctgat cggggatgta gacgtcttta agggcatgga agcaggcggt
     4381 atcgccatcg tcaacaccga gaagcccgac ttcgatgcca gggtcccgga gggcgtgaag
     4441 atctacacca tcgatgcgac caccatcgcg ctcgaagaac ttggtgtgcc catcaccaat
     4501 acaaccctga tgggcgcttt cgccgcagcc accggcgaga tccagctgga acctctggaa
     4561 cacgcactgc ggaaccggtt ctccggaagc atggccgaca aaaacgtcaa ggcagcagag
     4621 cgcgcatata atctggctgg aggtaccgcg taatggcact gcgtgtcggt tgcgccgcgc
     4681 ctccgggcag ggctctcgat aataagactg gcgcctggcg ggtcttcaaa ccggtctttg
     4741 aacccgaaaa gtgcacgaaa tgcggcatgt gcgccatggt atgtccggaa gggtgcgtcc
     4801 acgagacgga agagggcacg ttcgaacccg acctcgacta ctgcaagggc tgtggcatct
     4861 gcgtggaaat atgcccgaag aaaggtatcc ggatggagaa ggaggagaaa taatgctgga
     4921 atttatggaa gggtcgcatg cagtggccga gatcgtgaaa cgctgccgcc cgcaggttat
     4981 cgccgcctat cccatcacgc cgcagacgca catcgtcgag gatcttgctc agatggtggc
     5041 gaactgtgag atcgatgccg aatacataac ggtcgagagc gagttctcgg ccctctccgc
     5101 ctgcctcggt gcgagtgcag cgggttcccg ggtctattcg gcaacgacct cgcaggggct
     5161 tgccctgatg tttgaagtct gcttcaacgc ggcgggaatg cgccagccga tcgtgatgac
     5221 ggttgcgaac cgctcgctcg gtgcgccgct ctcgatctgg aacgaccagc aggactcgat
     5281 atccctgcgc gactccgggt ggctgcagtt ctatgcggag gacaaccagg aggctgccga
     5341 tcttcacatg atcgcgtacc gggtctgcga agaccacgac atcctcctgc cggcgttcgt
     5401 ctgcttcgac ggattcatcc tcacgcacac ctacgaaccg gtcgcactcc cctcccagga
     5461 ggaggtagat gcctatctgc ccgcctttac gccctaccag cggctggacg ctgaggcgcc
     5521 gctgagcatg gggatgtacg cgacgcccga gtactacatg gagttccgct acgagatcga
     5581 ccgggcaatg caccgttcca aacaggcatt catcaaagcg ggtcgtgagt tcggcgagca
     5641 gttcggcagg gactactccg ctctcgtgga aggctatcgc cttgaggacg ccgataccgc
     5701 gctcgttgcc atgggctcca tctgcggcac cgcaaaggac gctatcgacg agatgcgcga
     5761 gcacggccgg aaagtggggc tcttaaagat ccggacctac cggccattcc ctgcccggga
     5821 gatcgcggat gcgctcaaag gcgtctcgac ggtagcggtg ctcgacaaga acatctcgct
     5881 cggcagcggc ggggctgtcg gcatcgaggt gaaagcggcg ctcagcggtt ccagtcaggc
     5941 tgtatacgac tatatcatcg ctctcggcgg gcgcgacgtg aggaagaggg atatcgccgc
     6001 ggtcgtcgac ctcgccgaga agggggaagg ggatctattc tatggattac ggacggaggt
     6061 gctctgaatg gctgaagtag agcagggatg cgagctcttc tcggccggac accgggcatg
     6121 cggagggtgc gggccggcgc ttgcggcgcg tctcctcctc aaggcgacag gaaagaatgt
     6181 catcatcgcc gctgcgaccg ggtgcatgga ggtcttctcc acgccctatc cggagaccgc
     6241 gtggggggtt ccctggatcc actcgctctt cgagaatgct gcagcggtcg cgtcggggat
     6301 cgaagcgtcg ttaaagaggc aggggcgcag cgagaaggtc gtctgcatcg caggcgacgg
     6361 ggccacgttc gatatcggga tgctctgcat cagcggcgcc ttcgagcggg gccacgacat
     6421 cacctacatc tgctacgaca acgaggccta catgaacacg ggcatccagc ggtcgggcgc
     6481 gacaccctac ggtgcgagca ccaccacgag ccctgcgggg aagtgctcgc ccgggaacgt
     6541 gcaccaaaag aaggatatgc ctgcgatcct cgctgctcac ggtgcgccct acgtcgcgac
     6601 cgcctcgatc gcctacccca acgacttcat ccagaaggtc gagcgggcca tcaacacccc
     6661 cggaccctgc tacatccagg tgcacacccc ctgctgcacg gggtggggct ttgagtccgg
     6721 tgagacgctc gccatggcca agctcgccat cgagaccggc ctctgggtga actacgagct
     6781 ggtcaatggg caggtggaga aggcgaagaa ggtgaggcgc aagcccgtcg aggaatacct
     6841 cacgaaacag aagcgtttcc ggcacctctt caagcccgcc cgccaggacg acatgatcgc
     6901 gaacatccag gcgatcgccg atgcgaacgc ggagcggttc ggcatcgaca tcaagaagaa
     6961 agaatcgtca gaataaagta cagcccgaag agtatcatgg cgagtccgct cgcctgcacc
     7021 acttttttat aatgggggcc aagcacgttt gctccgcgtg agaccgcaaa ggcgaggagc
     7081 ccgagccaga cgatatccgc accgatgtgc ccgagataga attcggcaaa cgacgggtgg
     7141 agcgcgagaa atccgacacc gaacgtgagc caccataccc accagtaggg gttgccgagc
     7201 gtgcaggcca ccccgaaggc gaccggggtc cgggtctcct cgttcacggc gacggcatct
     7261 cccgcgtgaa ggaactgcgc gacaccgagc gcgatcagga cgcccgatcc gaggagggcg
     7321 atgaggtcga tatacgggac gtcgcgcaca ccaaaggcta tggctgcaac catgacggct
     7381 tcgggtatgc cgtgccccgc taccagccct gcaacgaacc gcccgggttc ccgtgacccg
     7441 aggccgagga ccgaggcggt catggggccg ggcgaggcgg ccccggagaa cccaatcaga
     7501 aacgatgcga cgacgatatc aagcatcagc cttctctcct ccgtgagatg aggaggggca
     7561 ggagcatgac aagcgcgacc gcgagccata tggtgccgaa ggtggtgagc agaatctcgc
     7621 gatccggggt gtagaagcgg acctccacga ccgtcatctt gtcccaggtg acttccgtag
     7681 tcccgttcgg cccggacgag atgaccccgc cggggctgat catgccgatg agcgggttcc
     7741 tgacgtcaaa tccctccgga agggtgacgg ttatggcatg cggtgtgtcg aaggccgcga
     7801 caaggcggtt gtctctcacg ggggcctgat aggtgaccgt gtagttaccc tccgggaagg
     7861 cgatggcacc cctcccggtc tcccggtact cgaccgggcc ggagggggcg agcacctcga
     7921 taccctcgac ctggagcggg acccgctctc ccatcaggcc cggggtccag aacgtgtgtt
     7981 cggtgccggt gacctggacc gaggcctcgc aggaggtgcc gccggggagc acctggaaga
     8041 cggcctcctg tgccgccgcc ggggcacaga tcagtatcag cgcaaggcag agagcagcga
     8101 ctgcaggtcc gcgtcgatct cgaggggggg aacgcattgt cgtaccactg tttctggatc
     8161 ttttaagaga tgaccggtca ccacacagac gatccgctca tccttatcga tcaggccgag
     8221 ttcggcaagt ttccgtattc cggcgactga ggccgctgag gcgggctcga cgccgatgcc
     8281 ttccttcctt gcgagatccc gctgcatggc aaggatctcg tcatcggtca cggccgccgc
     8341 cgtgccaccg gttgcgcgga tggcgacgag cgccttctct gcattcaccg gggcgccgat
     8401 ccggatagcg gtcgcaatgg tctcgggtga ggtctctgga accagcacgt cgaggttctg
     8461 ttctatcgcc ttcacgagcg gctgcgaacc ggccgcctgg atgccggtca tcatcggcag
     8521 ccggtcgatg aacccgaggg cctcaagctc ccggagtccc ttgtagaccg cggagatgtt
     8581 gcccgcgttt ccgacgggga ggaccatccg gtcgggcacc tcaccgccga gctggtcgat
     8641 agcctcaaac ccgatcgtct tctggccctc caggcggtag gggttgaccg agtttaagag
     8701 gtagaggccc tggctgatgc agagctcgtg taccatctcg agcgctttgt cgaagttgcc
     8761 gcggatggag atgacccggg cgccgtgcat cagcgcctgg gcgatcttgc cgagggccac
     8821 ctttcccgca ggcaggagga cgacgcaggg gatgccggcc ttcgccgcgt aggcagcgag
     8881 gcttgcggag gtgtttccgg tgctcgcaca ggcgacggtc gtcatcccga gctcgcgggc
     8941 catactgacg cccacggtca tgccgcggtc cttgaacgag ccggtcgggt tcatcccctc
     9001 gtgtttggcg tagagttcac tgagacccag ttctctgccg atattcttta agtggtagag
     9061 cggggtgccc ccttcctgga gggagaccgg ttcgccctga accgggagga gctcacggta
     9121 gcgccagacc gagagggggc gccggttcca ttcatcccgg gagacgtcga tgccgtccag
     9181 gtcgtattcg acggccagca ggtgtccgca gcggttgcat gtgtagatga tctggtccgg
     9241 ggtgtaggtt gcaccacaat gaacacagac gagacggtac atagaaaacc gttgatcgcc
     9301 cggatacgta tagatatggt atcgggggtc gggacggatc gcttcaccgg tgccgccgct
     9361 cgcgccggac acgggagacg agcacgaaga gcagccagat gaccggagtg ccgacgagtg
     9421 cgaggcgccc gagttcctct ggtgatgtgg tgatcaggaa cggtgcgatg agcatccctg
     9481 ccgccgctgc cgcaagcacg atggcgaagg cagtcgggcg cacctcaaag tccccctctg
     9541 cccggatgcg gcccgccacc cgctgccggg agaaggggta gagccaggcg atgccggccg
     9601 gggtgcagga gtcctctacg aggtggagga cgcaccccac aaagaatgcg cacccgaagg
     9661 ccgggaggag cacgagaggc tccccaagcc aggtgaggat gaggcccagg tagcctccga
     9721 ggatcaggga ggtcagcccg acacccgcga atgagtggag cagcccgcgg tgccggtgcc
     9781 ggtaactctt ccggaggagc agcgagaata tcagggagag cgggtagtag atgagatacc
     9841 tgatggtata gccgaagatc ggaagcacca cggcaagacc gtttaagacc tgccctttct
     9901 tcccttttat tccgcctatt cgaccgttga atatcgctgc gtcgacggca tccgcgtccg
     9961 gcgcaagcga tccgatgaaa gtgccgaaga ggaggacggc gatcagggcc gggtcgagga
    10021 cgggagccca gggagcaata agaagtccgg ccgtggcgag actgagagat acgtgctggt
    10081 caccccgcat ggaatatctc tacaatttag agagtagact gtgataaagg tcccttctgc
    10141 ggtccgaaag tgaaggaagc cttagttggg cttcacggac cgctgccggg tcgacttcga
    10201 cggagaggag ttctgagccg gccccgcacc ggccgatgac ggtgccgtcc gggtcggcgg
    10261 cgagtgaccc tccacagtag atctcgcccg gcgcgccggc ggtgttgacc cctgccacgt
    10321 aatatcggtt atcaagagct cgtgcgggga gcagggtctc ccagtgaggc agccggcagc
    10381 agggccatgc ggccggcacc agcatgcact ccaccccggc gagcgcgtag acccggaaga
    10441 gttccgggaa ccggaggtca tagcagacgg caagcccgaa tctcgtcccg tcgaccgtga
    10501 aggtggcgat ccggtcgcct gccgtgtagt tgcagtcctc tccgtcagga gagaagaggt
    10561 gaatcttcgc gtaggcagcg aggagttcgc cgtctgcacc gagcaccacg gcggtgttcc
    10621 tggggcggcg cccccgcacc gcttcgacga tcgaacctgc gattgtgatg ccgttctttt
    10681 gcgccatctg cctgaatgcg gcagtgagcg gaccctccag gggctccccg gaccccggtg
    10741 ggatcttcgc cgaccacccg gtaacaaact gctcgggaaa acagatgagt gacgccccgg
    10801 ccgctgcggc ctcttcagcc atccggccgg ctgcatcaag catccccgcc gggctgctct
    10861 ggccgccggc aatctgggca agcgctatct tcgtcatcgt tgggatgggc tggtgtggcc
    10921 cctgcaggag agaagaagca tggtggcgcc gatgacgacg agggcggcga gtgccaccat
    10981 gacgtacggg gcgaacggga taactactgc gaacatctgc gccccgatga gaattccgcc
    11041 tgcaatgatg caggtgctga tcaggaccgc tccgggaagc gtaaggtcgg ttgtcatgcg
    11101 gggactccgg tgagtacggg gaacgctgct ctgattccga tgaggatgaa ctggactgca
    11161 atcgcgatga ggagcatacc catcagccgg ttcacggcgc ggtactcgcg ctggccgatg
    11221 cgccggacga tgacgtcgga gcgttgcatc atgtagtaag tgatcccgat ggacgcgccg
    11281 gcagcgagcg gtacgacggc aattgcggcg agactcatgc tcatcgcctc gttcatcagc
    11341 acgatcgcgg tggtgatggc gccgggcccc gtgatcatcg ggattgcaag cggcatgacg
    11401 gcgatgtcgt ccgcgtcctg cccctcgtac ttctcggtag cggtcatctt cgtccgtgaa
    11461 gtctttgcgt agaccatgtc cataccgatc ccgaagagga ggaggccgcc tgcaatgcgg
    11521 aaggcttcga tggagatgcc gaagagctgc aggatccagc cgcctgcgac ggtaaatatc
    11581 aacaggatta ccagggcgaa ctgggttgca tcacgagcga ccttgagttt tgccccctcg
    11641 tccatcgtgc cggtgaggga gacgaagatc agggtggctg cgagcgggtt cactacgatg
    11701 atgatcgaag agaaactcag cagcgcaaaa ctcaggaggt ctaccattcg tgatcaggta
    11761 tgcgacaggt ccgatattag cacttgtggt ttagaggggg gatgggtgat ccgtttctcc
    11821 ggaaacctgc tccggagaag attttttccc ggcaggtcgc gttggtgtgg ggttatccct
    11881 gcgggtgctt cgctgacggc ttgcggttcg gtggggacgg gctttatctc cgcggggctc
    11941 gcgggg
//
LOCUS       DAJB01000231            4153 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_189799, whole genome shotgun sequence.
ACCESSION   DAJB01000231 DAJB01000000
VERSION     DAJB01000231.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_189799"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          811 a         1262 c         1302 g          778 t
ORIGIN      
        1 gggccgggtg cggggctcga cggagacgag ttccttcacc cggacctgcc cgtccttgac
       61 gacgccggtg ccgaaccggt tcttatagga gacgatgacc gtcccgtcgg ggacggcggt
      121 cgccaggggg aaccgtttcc cgccgatacg gcccttatgg gcgctcacgg cgggctcggc
      181 ctcaaggtcg atgatgcccc gggttgcgtg ctgcaggatg tgcgggagcg cttcgggggc
      241 gatgtcgagg ctgaacttcc gggcgatcgg gtcgaacgag agccagccga accggtcgcc
      301 gtgcatgatg acgaggtccg cccggtcgac gccgccggtc ttgtttaaga gcacgacgtg
      361 gggcagggga atatcgccga actgttcggc aagaagccgc cggatcaggg ccatatctgc
      421 ggcaagggcg ggccggacgt cgtgcggctg caggagaggt atctccctga ctctggtttt
      481 gcaggcgcag gtgcgcccga tgagcgggac gttgcactgg tcgcaccagt agaggaactt
      541 ttttgctgcc ggttcaggta tcacttgaag aatagttggt tgctgcagac aataagtgaa
      601 ccggtgacga aaaaaccgcc cgggtgcggc cggaaacccg ggcccgggca gggatcccgc
      661 ggcgtgcagg cggccgtgag gtgggggaag agacggcccg cctcacggcc gcccgagcag
      721 caggacgaga ccggcgccga ggacgaccag caggacccct gcaaccagga agaagatggc
      781 atcgagatat ccctgcatct ggattccggg gaaatccggg ccaggctacg tgacgtttat
      841 caggacccgg gcgtcggcgc cggagaccgg gtcgacggcg acgaggagga gcctccccgc
      901 cggcagctcc gaggaggcaa tcaccccctg aaagtgccgg gtgtcaccgt cggagaccgc
      961 gcgttcatcc tccgggaccg tataccctgc gacgagcgac cctcccggga cgctgtagat
     1021 ctgcacgtcg acgctctgtg gggggcggta gaagctctcg tcccggctcc aggtggcgga
     1081 gacgctcagg ttctcccccg gggagagggt atcgtgcgat accgagaccg tgaggagagg
     1141 gggcagcgga ggcgtcgcgc ccgagacgga gcagggcatg atgaggagag cggcgaaagc
     1201 catcaggagg aagaggcgga ccccgggcgg gcgacccggc attccatacg gcttcatacc
     1261 cgggtatctg cccggcacgc gataaccttt ccggcgtgtg gacagtcgat acagaccggt
     1321 cgggagatat acgaccgccc cgatgcccgg gttccgggat ccgggtatca gcgcaacatt
     1381 tattcgtgta aatgcgaaca gtagggagat ggcatcgtgc tgcgtaaaca atctcggtgg
     1441 acgggtgctc ctcctgggcg caactcccga ccaggcaaac gactacgtgc gaaagcactg
     1501 caaagagtac tacgagatac ccccgggttt cgtgttcagg gacgtccgga tcctcttaaa
     1561 aagccccatg ctcgtcggca tccaggtccg gaagcagaag gtcctgctgc cgtttatgaa
     1621 acgctgcaca ggcctcggga cgatactcta cgagatcgcg gcaaaggagg cggacatcga
     1681 tttcatccgc gaacacctcg cgaaagtctc agaatgaggg gccgggtgac gatgcggccc
     1741 cgccacgctc cagatgcccg gccgataccc gggtgagcag accggccgcc gtgcgggaga
     1801 tgccgcaaaa ttctacggcg aagccgggcc gcaaccggag cagtttttgc ggatcgctcc
     1861 cgacgaccga ccgatgcgcc cgcggtgggg ccttgctttc ctgtttaccc tgtttcgtcg
     1921 tatcctgccg cccctcccgg gcggctgacc gaacgctata taccccctga cgggaaaatc
     1981 ttatagcgat catcgtgggc attaaggagt gggtagtccc tcaagataag attttttttg
     2041 acctctttga cagaatggcc cggacggtcg tctccgcagc cgatctgctc gtcgagttcg
     2101 tcgaaaacta cgagaacgtg aaagagcagt gccaccggat gaagcagatc gagcaccagg
     2161 gagacgagat cacgcatgag atctacgagc agttgaaccg gacgttcatc acgccgctcg
     2221 agccggaaga gatctcccgc cttgcgtcgg cgctggacga cattctcgac tacatcgacg
     2281 gcaccgcaca acagatgtac agctacggca tcaccaagac cgacgactcg atgatcgaac
     2341 ttgcaaaact gatccagttg agcgtcgtcg agattgagaa ggcagtcaac aacatccggt
     2401 cggtcaagaa cccgagcctg atcgaggagc ggtgcattga ggtgaaccgc ctggagaacg
     2461 tcgcggacaa cgtcctcggc catgccatca gggacctctt caagacggaa gatgcgatca
     2521 ccatcatcaa actcaaggac atctacgaaa acctggagat ggcgaccgat aagtgcgagg
     2581 acgtcgcaaa cgtcttaagc gacatagcca tcagacactc ctgaccgcta tggacccgat
     2641 catcatcctc ggcattcttc tcgcgctact ctttaatttt gcaaacggtt tgaacgatgc
     2701 cgcaaactcg atcgccacca tcatcgcgac caaggccttg acacccctgc aggcggtgct
     2761 cctcgcaggg gtcttcaatc tcctcggccc ccttctcttc accacggcaa ttgcagcgac
     2821 catcgggagg gcgatcgtcg accccgtctt cctcacgccg tcgctgatcc tcatggccat
     2881 ggccggtgcg gtgctctggg tcctcgcgac ctcatacctc ggaatccccg tatccagcag
     2941 ccatgccctg atcggggggc ttctgggtgc cggcattgca gctgccggaa cgggggcggt
     3001 aatctggccg tcgcttgcgg tcatcgggca ggtcctcttc tacggcctca cgggtgtaat
     3061 cctcggtgcg atcgtcacgg gtgcatttgc gcttctaaaa ggcgacttca agccgtggaa
     3121 cctcatcctc ggcggactca tcggggtgac ggtcgtcatc ccgcttgcca tcgcaacggg
     3181 gttgttgaag gtcagcggca ttctcggggt tctgatcttc atcgtcatct cccccatgct
     3241 cgggttcata gccgcgtttg ccctcggcac tctcgtcgcc cacatcttcc gcaaccatcc
     3301 tccgcggcgc ctgacaggcc tctttcgcaa tctccagatc ttttcggggt cgtttcaggc
     3361 tatcggccac ggcagcaacg atgcccagaa cgcgatgggg atcatcaccg ccatgctcct
     3421 cgccggcggg ctgatcaccg agttctccgt cccgctctgg gtgatactcg catcttcgct
     3481 cgccatctcg ctcgggacgc tcttaggagg atggcgggtc atcgataaga tggccaacaa
     3541 gatcaccagg atccgcccct accaggggtt ctccgcatcg accgccgccg ggggcgtgct
     3601 ttcgctgatg accgcctttg gcatcccggt ctcgacgacc catgcgacaa ccggcgcgat
     3661 catgggcgtc ggcgcaacgc gcggctactc ggcggtcaag tggggcatag tccgcgagat
     3721 cctgatcgcg tgggtcctga ccatcccggc ggcggcggtc gtggccggga tctgttattt
     3781 catcggccat gcgttgatca gcggggtttt ttgagggagc cggagtcgac cataaccgtc
     3841 ctggatcgaa gaagagtggg tcgggtgtcc cggccaccag gacgaattat ttcccggggt
     3901 tcagccctca ttttttcatg ccgtcggcgc agctgagtcg attatatagt ggaaaaacaa
     3961 atgataacgt agttgttccc catcctatgg ggatgaacct gtatcgtaca atgaaacagc
     4021 cgtccggctt ttcacgttcc ccatcgcgtg gggatagtcc gcactggcgg cggattcttc
     4081 ttctctttct ggccgggtga tcccgggaaa agatggtcgc tattacaacc cggagggcgc
     4141 cgccggggcg aac
//
LOCUS       DAJB01000232           14390 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_14737, whole genome shotgun sequence.
ACCESSION   DAJB01000232 DAJB01000000
VERSION     DAJB01000232.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_14737"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    9175..9189
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2710 a         4590 c         4571 g         2502 t
ORIGIN      
        1 catgatgacc tagtgatcaa ttgattacca tacatgataa aggtaacggt tgatattctg
       61 gctttaatta tgcaaagaat tgaggagggg tgagattgaa attattgatt ctggcaccta
      121 gtgatcaaaa agacgctgtt taagtctcga agacgggggt cgtgccagcg gtcgagcgtt
      181 gcggcaggat cgcgggatgc ggccccatcc ggatatgcga atacggccag taacgcggag
      241 catcccgtca ttttgctctc cgcgcaggca ccagaatgcc tcgatccgct ccggggatga
      301 cggttgccaa tttcccgagt atcggagcac ggaaggggcc tgaggatccg cgggtattcc
      361 ggttcgacga catttatata cggaaacagc caactaccac cgatgacaga cgagggctca
      421 aatccaccgg cggtctgtct gggattcgac attcactatc cctgctccct caatcccaga
      481 tttcaacccg acgtggtaaa gggaaagaga aacccgaagg atagctactt caatccggac
      541 gcgaaggagg acctgggacg ggtcatcgac cgctccttca cgccgacgac ggagatcctc
      601 ctcgatcttc tcgatgacgg gctgaagtgt gccgttagca tctccggggt ggttgtggag
      661 cgcctcgagc agtggtaccc ggagatgctc gaacttctct cccgtgcggc aacccaccgg
      721 aacgccgaag tcctcgccgg gacctactac cacagcgtcg ccgggcggtt ctccgacctc
      781 acggagttcg tggacgaggt ccgcatgcac cgggacctga tgaaggagca cttctcggtc
      841 acgccgacta cgtttgcgca taccgactac ccgatcaccc cggccaccgc agaggcgctt
      901 gccggggccg gaattgaggc ggccatcatc gagggaggcg aggggcccgc tcttgagagg
      961 gaccaaaact acacctacac ctaccggggc ctcccggtcc tcgtcaccca ctgcgatctc
     1021 tccgacgaca tcgccgtgcg gttcccgtgg cgagagtggg accaatggcc tctcacggcc
     1081 gaccggtacg cggggtggct cgccgcctct cccggcgact gcatcacgct cttcctcgac
     1141 taccgcatct tcggggactt catcggcccc gaggcgggca tcctggagtt cctgcgtgcg
     1201 ctcccgtccg ccctcgccgc ggaggggata gagaccatac gcccctcaga cgccgcccgg
     1261 cttccaccgc acgaggagat cggggagacc ggcgagccga ccggcttgca gggccggcag
     1321 aggaccatca tgcagcagtc cgccatcgag gcgctcgagg aggccggggg cctggtgaca
     1381 gacgcggagg tctggcgctg cctccttgag accgagcata ttcgccggat ggccatgcgc
     1441 tcgacctcgt gcggaaaacc gctccacgcc gtcagccacc aggcgaccta cgactacttc
     1501 gcctcattta tgcggatcct ctcgcacgcc gaggagcggt ctgcagccat cgcctcgtca
     1561 cccagggccg ctctctcgct ccgttgcgtc cccccggata aggcattcta cttctcgtca
     1621 tatgaccggc cggccgggta cgccgcccac agtctccagg aactcgcaaa tatgctcgag
     1681 tttgtcccgg acgatgtcat ccggtaccac gtggagcggg aggactttaa ccgctggatc
     1741 gcccaggtca tcggcgatac gcaactggcg gagaagatct ggggtatctc cgaccgcacg
     1801 agactccgaa cgatgattca gaagaggatc cacaaactat ggaaacgctt aagttagcat
     1861 tcttctgctg ggagtcgctc cactccacgt tcaaggtggg cggccttgcg ccggcggcga
     1921 cccacctcgc cgagaacctc gcccggaagg ggcacgaggt ccacttcttc acgcggggag
     1981 agggggacga tgcgttgatc aacaatgtcc actaccacta ctgcctgcct ttcggcgaca
     2041 acatcatcga gtactgcagg accatgagca acatgctcgt ggatgcgttc cgcgcccatg
     2101 acgccccgcc attcgacatc ctgcacttcc acgactggca cctcgtcgag gccctgcaca
     2161 ccctccgcgg caggaacacc gtcctctcct tccactccac cgaatacggg cggcacgggg
     2221 ggaatttcgg gggctggtgg gagttccagg agatatccgg caaagagtgg tacgggggct
     2281 acatcgcaaa ggcggtcgca accgtctcga actcgacgaa gaccgaagta atgtggctct
     2341 acaacgtccc cgaatggaag gtcaccgtca tcccgaacgg gatcgacccc gacacttatc
     2401 atactcaggt cgacccgggc gaggtgaaga aacgctacgg catccacccg ctcgcgcccg
     2461 tcgtcctctt cgtcgggcgc ctcacctacc agaaaggacc cgacctcctg atgcaggcca
     2521 tacccgagat cctcgccaaa cgctgggacg tgcagttcct cttcgccggc accggggata
     2581 tgcgcggtta cctggagggg atggcgcacg gtcttcccgt gcagttcctc ggctacgtct
     2641 ccgatgagga ccatatccgg ctcctgaacg cctgcgacct cgtcgccgta ccgagcagaa
     2701 acgaaccgtt cgggctcgtc ctcacggagg cctggagcgc ggagcgctgc gtcgtcgcga
     2761 ccgatgtcgg ggggctctcc gagaatatcg ataattacgt aaacggcatc aaggtccccg
     2821 tccggccgga ctcgatcgcc tggggaatct gccacctcat cgacgacccg gcctacatgc
     2881 agaaactcgg gagggccggc cgcaggaaag tgatagagaa gttccagtgg ggcgtcgtca
     2941 cggagaggat gctcggcgtc tacaggagaa ttctctccag cggcgatacc cggccgggag
     3001 ggtgagaata tgcagcaggt gctaccggat atcacgaaga ccgggagcct catcaccgac
     3061 tacgacgttt acctctttcg gcaggggaac cactcccgcc tgcacgagag aatggggtcg
     3121 caccccgccg tcgccgatgg tgtgcggggg accttctttg cggtctgggc gccgaacgcc
     3181 gcggaagtct cggtcatcgg ggatttcaac gggtggacgg cgggccggca tccgctccag
     3241 gcacggagcg acgactccgg catctgggaa gggtttatcc cggatatcgg ccacggaacg
     3301 ctctacaagt accatatcaa aagcaggtac aacgactatt ccgtagagaa gggcgacccg
     3361 ttcgcatact tctgggagat cccgccaaag accgcgtcca tcatctggga cctcgcctat
     3421 gtctggcggg accgggcgtg gatgcgctgc cgggaggagg ataacacccc ggacgctcca
     3481 atatccgtct acgaggtgca ccccggctcg tggcggaagg tgccggagga ggagaaccgg
     3541 tctctgaact accgggagct ggcgaccgag ctcgccgaat atatgcttga agccgggttt
     3601 actcacgttg agttcctccc cgtcatggag cacccgttct acgcctcctg gggctaccag
     3661 acgctcggct actttgcccc gacgagcagg tacggcaccc cccaggactt catgcacctt
     3721 atcgaacatc ttcaccagcg ggggatcggg gtgttcctcg actgggtgcc gtcccatttc
     3781 ccctccgacg gctacgggct ctcctacttc gacggtacgc acctctacga gcacgctctc
     3841 cccggccagc gctaccaccc cgagtggaag agttacatct tcaatctcgg caggaacgag
     3901 gtccggtcgt ttcttctgag cagcgccctc ttctggctcg agcgctacca cgcggacggc
     3961 ctccgggtgg atgcggtctc ctcgatgctc tacctcgact acgcgcggag cgccggggag
     4021 tggataccga acgtctacgg cgggagggag aacctcgagt cgatagggtt cctccggaag
     4081 gtgaacgaca cggtccacgc aaacttcccc gacgtgcttg tcatcgccga ggaagcgact
     4141 tcctggccgg gggtgaccgc cccgaccgcg accggggggc tcgggttcga cctgaagtgg
     4201 aacatgggct ggatgcacga caccctggac tacttcgtgc tcgacccggt cttccggaag
     4261 taccggcccg acctcctgac gttcagcata tggtatgcgt tccgggagcg ctacatcctc
     4321 cccctctcgc acgacgaggt cgtccacgag aagggttcgc tcgcaggaaa gatgcccggc
     4381 gacgagtggc ggaaacgggc gaacctccgc ctcctctacg gctacatgtt cacgcatccg
     4441 ggcaagaagc tcatcttcat gggcggtgag ttcgggcagt ggtcggagtg gagccatgat
     4501 accgggcttg aatggggcct cctgcagtat ccttcccacc gggggctcct ccggtgggtc
     4561 accgacttaa atcaccttta ccggcggatg cccgccctcc acgagcggga cgccgatccg
     4621 gaggggttcg aatggatcga cttctcggac gtggagaaga gcatcgtcag ttacctgcgg
     4681 cggggacact caggcgacga agtcgccctc gtcgcctgca actgcacccc ggtgccgcgc
     4741 tacggctacc gggtcggcgt cccgttcggc gggttctgga aggaggtgct caacagcgat
     4801 gcggtcgagt acggcgggag cggtgtcggg aacctcggcg gggtggaggc ggagccggtc
     4861 ccggtgcacg gccggccgtg gtccctcccg ctcaccctgc ccccgctcgg cgcagtcatc
     4921 ttcgctcccg agggggcgtg accgtggacc gtgccgtctg catccacggc cacttctacc
     4981 agccctcaag ggagaacccc tggctcgggg aggtgggggt ccagcggtcc gccgctccat
     5041 accacgactg gaacgaacgg gtggccgcgg agtgttacgc cccgaacact gccgcacgca
     5101 tccttgacgc ggacggcatg atcgtggaag tcgtaaacac ctattcacgg atcagcttca
     5161 cggccgcccc gacgcttctt gcctggcttg agcggcaccg cccgggggtt taccgggcaa
     5221 tcatcgaggc cgaccgggag agccgggagc gctacgccgg ccacgggtcc gcgatcgcct
     5281 gctgctacaa ccacctcatc atgccgctgg caacccggcg ggataagcaa gcccaggtcg
     5341 cctggggggt ccgcgacttt gcagcaaggt tcggccgggc tcccgaaggg atgtggctcc
     5401 cggagacggc ggtcgacctt gagtccctgg acctgatggc cggggcgggg atccggttca
     5461 cggttctcgc cccccaccag gccgccggag tgcagccgat cggggcggag gactggacgg
     5521 acgtctccgg cggccgggtc gatacgacaa tgccctacct ctgccgcctc ccctccgggc
     5581 aaagcatcgc ggtcttcttc tacgacgggg cgattgcccg cgacgtcgcg ttcggcgacc
     5641 tcctctccga cgggcggcgg ttcgccgggc ggctgctcgg cgcgttctcc cgggggagcg
     5701 agcgcccgga actcgtgcat atcgcgaccg atggggagac ctacggccat caccacgcgt
     5761 tcggggagat ggcgctcgcc tactgcctcc gcaccattga ggagggcggc gacgcacgcc
     5821 tcacggtcta cgccgagtac ctcgccgctc acccgcccac gcacgaggtc gccgtccggg
     5881 agaggacgtc ctggagctgc acgcacggcg tcgggcgctg gcaaggggag tgcgggtgcc
     5941 atagcgggac acacccgggg tggtcgcagg catggcgcgg cccgctccgg gaagccatcg
     6001 tcatggtccg ggatgctctc gccccccggg tcgcggagga gatggcggcg cacgtccgcg
     6061 atcccgcgga ggcacgggac gacgctgtcg gcctcgtcct ttcccggtgg tccgacgagg
     6121 ccatggcggc gttcttctcg cggcacgcgc ccgggagcct ctctcccgac gagcggcggc
     6181 gggtgcttgc tctccttgag ctggaacggc aggtgatgct gatgcagacg agttgcggct
     6241 ggttcttcga cgatatcgca gagccgggga gcgtccaggt gatgcggcac gcgaagcggg
     6301 cgatggacct tgccctggag accatcgggc tcgacctcga gccggcgttc atcggtatcc
     6361 tccgccgtgc cccggcaaac gacccggagt atacgaccgg tgcggaggtg tacgaagccc
     6421 tggtccggcc ggcggcggcg gaccttgccc ggatcggggc aggagccgcc ctctacgcgc
     6481 tcttcgacat cgaaccccct gcggtcgcgt cggggatgta tgccgttcac ggagactggc
     6541 gctaccggtc ggacgcgggg ggttggcgcc gcctcctcgg gaccgtccgg gttcgggccc
     6601 gggcgacggg cgatgaggcg ctcctcgatg cggtcgcact cttcgccgga atagaccgtg
     6661 ttttctctgg cgtgctcgag cccggcgacg atgaggggtt ccggacggtg tgggtgggga
     6721 tgatggggcc cgaccccgca ggcgccgtag ccggggcctt tacccgggtc tacaccgcgt
     6781 ccgacctctc cgacgaggag cggcggacaa tcgcccgccg gaccgccccc gggatcgggc
     6841 aggccgtctg tgcggtctac cgtgacgcgg cagcgctgat cggcgtgctc gacgacctcg
     6901 ggatccctgc gccgggagcc gccgcccatc ttctggcgca tgcctgcacc gagcggctgc
     6961 tcgcgatgct cggagggggg tgcaccgacc cggatcgatt tttcgcgctc gcagaggaga
     7021 tgagagcccg ggaccttgcg cccgatcttg cgggcctcgg ggaggcggcg agcaggcgga
     7081 tcgcgctctc tttgcaggcg gcggcgaccc ggcccggtga cccggctccg ctcgaggaga
     7141 tcgtccgcac cgttagatgt acgaaagttt ttccgttgac gctctccctc tgggagagcc
     7201 agaacctctg catcgggatg cgcgggcgct attttggaat gcgggagcgt gctggccggg
     7261 gtgatgggga tgcagaacgg tgggcggagg ccttcaggag ggcggcggca tgcctcggcg
     7321 tgaaggtggc gtaaggaggg gcggagcatg aaccggcggg gcagcggggt tctcctgcac
     7381 atcacgtccc tgccgtcggc gtacggcatc ggcgacctcg ggccggcggc gcaccggttc
     7441 gtagacctcc tcgccggcgc cggacagcgc tactggcaga tcctccccct caacccgacg
     7501 tgcccggcgt tcggcaactc gccctaccag agcacctcgg cgtttgccgg gaacacctgg
     7561 ctcatcagcc ccgagcagat ggctggcgac ggcctcctcg ccccggagga tatcgggaac
     7621 cctcccggtt tccccgagga acgggtcgac taccaggcgg tcctcgacta caagaacggc
     7681 ctctttaacc gggcgttcga gcggttcaaa gagcgcggcc ctgacttcca ttacaaggag
     7741 ttcgcggccg gcaacgtctc atggctcgac gactatgcca tcttcgtcgc cctcaaggag
     7801 cggttttccg ggaaggaatg gggcgactgg ccgtccggga tccgggaccg gcaccaggaa
     7861 gcgctgcgga agcacggtac ggagctcgcc gatcggatct tccgggagaa gttcctccag
     7921 tacgtctttg accgccagtg gaaggtgctc aagaaccact gccgcgagcg ctacatccag
     7981 atcatcgggg atatcccgat ctacctcact tacgacagcg tcgacctctg ggcgaacccg
     8041 gggctcttca aactgaacga gcagaagaaa ccggccgcta tcgcgggcgt tcccccggat
     8101 gtgttcagcg agaccgggca gctctggggg aacccggtct acaactggga tgcgcaccgg
     8161 gagcagggat tcacgtggtg ggagagccgg atcgaccgca ccctcaccct cgtcgaccgg
     8221 ctgcgtctcg accacttccg gggattcgtg cagttctggg aggtgcctcc cggtgaagag
     8281 accgcacgga acgggcgctg ggtcgacgca cccggccggg acctcttcac cctgcttgcg
     8341 agaagcaggt cgtgcctccc gatcatcgcc gaggacctcg gctacatcac ctccgacgtc
     8401 cacgagatga tgggccactt cggattcccg gggatgaaga tcctgacctt cgggttcgcc
     8461 ggcgacgtcg caagaaaccc tcatgcgccc cacaacataa cgaagaactg cgtcgcctat
     8521 acggggacgc acgacaacaa cacggtccgg ggctggttcg agcacgaggt ccccggcgac
     8581 cagaaggacc tcgtgttccg ctacatcggg ggccacgtga gcgcggaaga gatacaccgg
     8641 atcctcatgc gcctcgcgat gctctcccct gccgataccg tcatcattcc gatgcaggac
     8701 gtcctcggcc tcggcgagga ggcgcggatg aaccggccgg gcacggggga ggggaactgg
     8761 gagtggcggc tccggccgga gcagatgggg gaagaggcca tgcaggagtt tgccggggtc
     8821 acggggatct acgggagagg gtgagggggg gtcgaaaaat gggtttcgat taccgctaag
     8881 atacaccagc caagagacag tcaactaccc ccggttgaaa ccggtggtct ccttgcccgt
     8941 cgtatatgaa aaaatgtccg acacactgga ccgtgggaga atcatcacgc actgcccccg
     9001 ccctcagggg cggggtgcga tgggccatag ggccggagca tgagcaccgc aggtgcggag
     9061 gaggaggccg gaggccgggc ggggtgcgac ggacgggacg tccggagctt gagaagaccg
     9121 taggtcttcg ggtgggggtt acggccatct ttttcctagt actcgttgtc atttnnnnnn
     9181 nnnnnnnnnc gtcagccccg cccccaatgg cgattctccc acggtccgct ggatcgggcc
     9241 tttccctcat tttgaggatt cgccgactgc aacttgcacc agatgatgtt cccgctccgg
     9301 aacatcgccc tctaaactac cacagtcaag gggcaacact caccgtccat ttcctgcaca
     9361 aaattctgac cccccatctc agacccaccc ccatcgccgt cgcgctggcc acccccgtga
     9421 cagggatcac aggagatggc cgagcaactc cagcacccag ggcccccctc cccccctttc
     9481 gggtatcccg cagatgcccg gcaccccgta tgccgggaca gccgccctga tccacaccac
     9541 tctcagaaat cccaatatca gcggctattt tccgtttttt cagcccattt caaaaataag
     9601 caaacatata taataataaa catggacagt acaccatcca aaaaggggac agaattcatg
     9661 gagaattcca tacacgatcg gttcgaccac atcccggagc ggctctccgg gctcgtcgac
     9721 ctggcataca acctctggtg gagctggaac ccggaggccc ggatactctt caagcagttg
     9781 aaccggcagg cgtggaaggc gagcggccat aacccggtcc ggatgctccg cgagatgccg
     9841 gccgaattcc tgagccgggc ggcggagaac cctgcctact gccaccgcta cgacgtcatc
     9901 ctgcgccggt tcaggaagta catgagcgcc accgggacct ggttctccga ggagtactcc
     9961 ggcggccgcc cgttgacgat cgcctacttc tcagcagagt acgggctcca tcactcgctc
    10021 cccatctacg cgggcgggct cgggttcctc gctggcgacc acttaaaaga ggcgagcgac
    10081 ctcggcctcc cgatggttgc cgtcggcttt ctgtactcgc agggctacct gcaccagcag
    10141 atcgactccg acggctggca ggagggcatc accgaaccgc tcgaccgcga tgcagcgccc
    10201 ataacccggg tgcttgattc cgacggcgaa gacctggtgg tccgcgtccc gcatatcgac
    10261 ccgcccatct acgtcgccgt ccggaaagtc caggtcggca ggataccgct ctacctcctg
    10321 gatacggaca ttccctgcaa cgactcccgg aaccggggca tatcgtcccg gctctatgcg
    10381 ggcgaccagg atctgcgcct ccggcaggag atcgtgctcg gcatcggcgg ccggaaggtg
    10441 ctccacgccc tcggcatcgc atacgcggcg gtgcacttaa acgaaggcca cccggcgttt
    10501 gcgctgctcg agcgggtccg ggagcgggtc gagcggggga tggagttcga ggcggcgctc
    10561 gatgaggtgc aggcaacgtc catcttcacc acgcatacgc ccgtccctgc gggccacgac
    10621 gtcttccccg tcgacctcat cgaccgctac ttcaagacct actacacggc gctcggcatc
    10681 gaccggacca ggttcctgca gctcggagtc cacccggaga gccccgcggc aggcttcaac
    10741 atgaccgcgt tcgccctgcg tgcgtcggcg caccacaacg gcgtctcgcg cgcaaacggg
    10801 gccgtcgcct gcgatatgtg gaagtgcctg tggcccgggc cccgggcgac ggtgccgatc
    10861 gactacgtca cgaacggcgt ccatgtgcct acctggttaa acccccggat gaaggagctc
    10921 tacgaccggt acatcgggcc gacctctccc gactggctct cggagcacga cgacccggtg
    10981 gtatgggaac tcatcgacga ggtcccggac gccgaactct ggcacgtcca tctctggctg
    11041 aaggcgaaac tcctcaaccg gatccgggag cgggagcgga taaagtggac cgcgcaccgc
    11101 ggcggggcgc aaaaccccgc tgccgagggg gcgttcctca acccctccgt cctgacgatc
    11161 gggtttgccc gacggttctc gacctacaag cgggcgcatc tcgtcttcga ggacctggaa
    11221 cggttaaaga gcatcctgaa caatccgtgg tatcccgttc agatcgtctt cgccggcaag
    11281 gcccacccgg ccgacaacga gggaaagcag gtgctccagc agatctacca ctacacgcag
    11341 cagcccgagt tcggagggcg tgtcgcgttc attgaggact acaacgagca ggtggcccga
    11401 tacctggtgc atggcgtcga cgtctggtta aacaacccgc tgcccccgat ggaagcgagc
    11461 ggcacgagcg gcatgaaagc gtcgttaaac ggggtcctga acctctccat cctggacgga
    11521 tggtggaccg agggctataa cggcagaaac ggctgggcgt tcgggaacga ggcgacgacc
    11581 tgcggcggga ggaatgccgt cgatgccgcg gcgatctacg acctcctgga gaaggaggtc
    11641 gtcccgctct actacaaccg ctccatcgac gacgtccccc acggctgggt gaagatgatg
    11701 aaggagtcga tcaagagcaa cggcccccgg ttctcgtccc gccggatggt gaaggagtac
    11761 atcgcccgct actacccgtc tctcctgaag gccgccggga cggagtatgc ccggactccg
    11821 gcagcggcga aaccccgggc gacggtctgg aaaccgcggg tgcagggaaa aatcggggac
    11881 ggaagcgact gagctccgta acgtgcccgg agacctaccg ggagcgcttc gggacggctg
    11941 aaggccggcc gcctcttcgc gagacccgct tccgggtcgc ctccggtgca gcctggagtc
    12001 cccgctcttc gtgcggggcc ctgcccccgg cnncccgccg ccgggtctcg cgggaagctg
    12061 ccttgagccc ccgttcagta tgcggcgcca tcccgccagt ctttgcgacc cgtttccggg
    12121 ttgcctcgtc agcggatgcg aggcctcgtt tcactctcgt gatcgcattc atcggaagac
    12181 aggccaccgt gatggcaggg gatatgcggg agatcgcatt tgagggggac gatccctgca
    12241 tcgaagagct ggaacgcgag ctctatagcg gctcaatcac gtccgatacc gaactcgcgg
    12301 agcgggcgga cgagattggc gtgacaatcc gggtcagaga cgataaagcc aaggtctcgc
    12361 agcagggcgg ggtgctcatc ggcgaggtga ccgagacgga ggggtcggta acccgcagga
    12421 agaggctgta cgctacggca ggaagttacg caatcgccga ggcgatcgat tcccggctgc
    12481 gggtaacgca gaggggcagg gcaagcaact tcgtggtgct gggaaacgag gttacgaagc
    12541 ggattgccaa ccagtgcatc caggggatgt gggaaggagg gacgattcag gacgccatgc
    12601 ggctcctcat gctcaccatg cagataacgg cgagcgtgac ggcgtcggtg agcagaacat
    12661 tcatactggt gcataccgat ctcgcggcca acctggtcga cgccatcaac caggattcgc
    12721 ggaaataaag tatcgccggg aaggaggaat cccggagtgt gcccacctcc gctctggtgc
    12781 ggggccctgc gggaggccgg caaagaaatg caggaattat tggctgagat agttgtgctt
    12841 cttgagccag tctacctggg gcctgatgta gcggtagacg atatcgcatt tttcgtcctg
    12901 gaaactccag ggaacgacga tcaggagatc gccttcgcgt atccagagtc gcttcttgat
    12961 cttgcctttg atgcgccctg tgcgtgtgac gccgtcctca cagcggacac ggatgtggtt
    13021 tgccccgagc atcaggtcgg cccgggcaaa tatctcccgg tttcggttct tcggtaatcg
    13081 tacgcgtacg atctcttcac tctcgttgtt ctgtgatcct cgtgtattcg tcagttaatc
    13141 aactccataa caggcggcgg cgcaatccgc ctgatattta ctagaggtgt ataccaatga
    13201 cccgagcgac cattaaagta tctgtcccgg gaacacccgc gcaaccggga tccggcgggg
    13261 agagtccggg agatgcaggg cgctcccggg ttatcaggac cgggaaccct gctcaacgtg
    13321 atccccgccc ttatgtgcga cagagtgaac tatcctccgg caccccggac gatacggcgg
    13381 ggcgccggcc gcaaatcata tgccccccgg tgaggggcac gagggagacc tgcctgagag
    13441 tatgcacaat gtcccccctc tggccggaga ataccatgcc cgacgcagcg tactcctgag
    13501 acggcacccc ggttcaagaa gatatccggt ctaaaatcca ggatcaaggg cggtgaatgg
    13561 tctcttagaa agcgcaagta tatcagcaaa tgcgtccccg tatcgggtga gttgccccca
    13621 aaccgctgcc cgggttttcc tccgaagggt aattaaggtt tgagcgtaca tgggtgaaga
    13681 ggatctttga atggaaagca gcaagctgta cgtcggcaac ctcacctact cggtaaatga
    13741 agaacagttg aaagacttat tcggccagta tgggacggta agtgacgtca gagtcatcga
    13801 gcgcaaaggg ttcgggtttg ttgagatgtc cagcccagaa gaggctgagg cagcaaagga
    13861 agccctgaac aacacagtat ttgaaggccg caccttaaag atcgacgagg cccggcctcc
    13921 gagaccgaga agcgacttcc agcggtacta agcacttacc cggaagaaca gtattctgaa
    13981 tatcccacat ttttcaagag tcggggttca agggaacctc acctctgatt gtccggcgcg
    14041 tacggccccg ttaccagtct atatcgtcgc cccggtcggt gcgtagccac tcatccctcc
    14101 cgaaggtcgc atgtgattca tcgagccggt gagcgggtcg ggggatctct ctgtcgaagg
    14161 tactgccggg gaagggtcag gccagctcgc ccggcgcagc cgtcgccatc ccctgttcta
    14221 aacattctct cgccagatta ggggtgtttt ctccttaaag cacaaccaca tttttattca
    14281 ccaagagcta tgggggcggc cgtgtgtctc gctatgcggg atgcagtcga aacggcggtg
    14341 agaagtatgg cagaagaaag accttcaggt gaactacccc gccctgaagg
//
LOCUS       DAJB01000233            3594 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_81067, whole genome shotgun sequence.
ACCESSION   DAJB01000233 DAJB01000000
VERSION     DAJB01000233.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_81067"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          952 a         1037 c          961 g          644 t
ORIGIN      
        1 tgctcgtcac ctggggcggg tacaaggcat cgatcgcgaa agagaccgcc cgccaattct
       61 tcaagatccg gatatgggat gccgaggacc tggtgcggat ggtccagacc tattacgagc
      121 aactcccgga cgtcatccag gccgagctcc cattgaagag gatctgggcg ctggtccaga
      181 gcgaggaata acacatggca ttcggcggcg aacgaaacac tgtgcaggcc ccgttcctcg
      241 gatacgctca ggaagtcggg tgggactacc tcactcccga caaggcactc gatctccgcg
      301 gcggaaggac cgggcttctc ctccgcgaga catttcttgc ccagatggag cgcctgaacc
      361 ccggaatcat gacccgggag aaaggcgagg agctcgtcag gcggatcgag cggatacccc
      421 cgaacatcga gggcaacttc atcgcctggg aattcctcaa aggtctccgg acagtctttg
      481 tggaggccga gaaccgggaa cgaaacctca catttctcga cacggaagat atctccagca
      541 acaccttcca catcaccgac gagttcgcct tcaccaacgg gagccggacc atcagggccg
      601 acatcgtcct cctcatcaac ggcatcccgg tctttttcgt ggagaccaaa gccgcataca
      661 agatcgacgg gatagccgag gcgctcgacc agatacgccg ctaccatcgg gactgtcccg
      721 aactgctcgc aatcctccag atctacgccc tcacccacct cctccgctac tactacagcg
      781 ggacctggaa catgtcacga aagaccctct tcaactggcg ggaggagacc gacggagact
      841 tcgagacact ggtaaagacg ttctgcgaca gggaacgcat gatccggttc ctcaccgatt
      901 atatcctctt cacccggcag gacgacgaac tcaagaagat catcctccgt ccccaccaga
      961 tgcgggcggt cggaaaactc gtcgaccggg catcggatcc cgacaagaaa cgcggactgg
     1021 tctggcacac ccagggttcc ggtaagacct acaccatgat cgtcaccgcc cagcagatcc
     1081 tgaaaaaccc gatcttcgag aacccgaccg tcatcatgct cgtggaccgg aacgaactgg
     1141 aaagccagct gtacgggaac ctcaaagcgg tgggcatcag gcaggtcgag atcgtgacca
     1201 gcaaacgcca cctccgggaa ctgctccagg gggaccgccg gggtctgatc gtcacgatga
     1261 tccacaagtt cgacggaatg cccgcagacg tcaacaagcg ggagaacatc atcgtcctcg
     1321 tcgatgaagc ccaccggacc acgggcggga aactgggcaa ctacctgacc ggagccctgc
     1381 cggaagcgac ctacatcggc ttcaccggca cgcccatcga caggaccacc tatggaaagg
     1441 ggaccttcat caccttcggg aaaaacgacc ctccgaaagg atatctcgac aaatacggca
     1501 tcggcgaatc catcgcggac ggcacgaccg tccctctcca ctacacgctc gccccgaacg
     1561 aactcagggt ggaccgggca acactggaaa aggagttcct cgatctcgcc accgcggaag
     1621 ggatgagcga catcgccgag ctgaacaagg tcctcgagcg ggcggtcaac ctccggaaca
     1681 tgatgaagaa tccggagagg atgacaaaga ttgcacacta cctcgccgac cattacaggg
     1741 actacatcga accactcggt tacaaggcgt tcctggtcgc cgtggaccgc gaagcgtgtg
     1801 cgctgtacaa gaaagaactc gacaaggtcc ttcccgccga ttacaccgcc gtcgtctaca
     1861 gccgtgccca gaacgatccc gaggacctaa cgctctatta tctctccgat gatgaggaga
     1921 agcgggtcag ggtcgatttc aggaatgcgg aaaaacttcc gaaaattctt atcgtgacag
     1981 agaagttgct caccgggttc gatgcaccga tactctactg tatgtatctt gacaaaccga
     2041 tgcgtgacca cgtcctcctc caggccatcg cccgtgtcaa caggccgtac gaggattcgc
     2101 aggggaagac caaaccctca ggattcgtcc tcgacttcgt tggcatcttc gagaacctcg
     2161 aaaaagcact cgccttcgat tccagcgaca tcgagggcgt cgtaaaggat ctcggaattc
     2221 tgaagggatt gtttgtgaca ctcatcgccg aaggacagga gaaatatctc tcgattgcca
     2281 agggaaagaa gagggataag gccattgaag cgctgctcac ccactttctc gatgaggaag
     2341 cgcgtcacga attctaccag tacttcaaga caatatcgga gatatacgac atcctgtctc
     2401 ccgattcgtt cctgcggccg tatgtcgagg atatggaaaa acttgccagg ctctacaaga
     2461 tactcaagga caacttcgag cccggcatat cggtcgacag ggaattctcc cgaaagatcg
     2521 cgaagctcgt ccagaaacac tccgacagca ccgatatagg ggagaccacc gggatatacg
     2581 agatcgacga gaaactgatc tggcacctgg aagagagcga ggcctccgat atcgagaagg
     2641 tcttcaacct catcaagagc atccagagaa cggtcgagaa atatgccggc agctccccgg
     2701 tattggtctc tatcggagag aaggcggaag ggatcgtcca ggcatacaag gaccggcaaa
     2761 aatccacgca ggagacactc gaggaactca agaagatcgt agaggagatc aacgaggcaa
     2821 gggcagaaca ggaaaagatg aacatgactg tcgatacctt cgcgattttc tggatcatga
     2881 agaaggagcg tatcgagaat ccggaaggga aagccaggga tatgaagacg gtcctcgatt
     2941 cctaccccca ctggagggag agtgaaaaac aggaacgcga ggtgcgttcc agcctgtacc
     3001 gtattgttct cgcctcggga aaaccgaatg tccaggaagc aaaagagacc gtaacgacga
     3061 taataagagt ccttcggcag gcatcggaat gatctccccc gcgtcgttca aggatgaagt
     3121 ccggatatgg gcagcgacaa tcggtgtgga agcccgtgag atccatatcc gcaaaatgaa
     3181 acgcaaatgg gcaagttgct catcgagagg acggttgaca ttcgatcccg aaatccttgg
     3241 actgccccgg aacgaccgtg acgaaaagat cgtccacgaa cttctccatc tgaagtatcc
     3301 gaatcatggg aggatgttcc gggtcatgtt gaatgcctat ttgcaccgtg ccgaaggaga
     3361 agagatacga gaaacctcca ttgattccgg ataaaattca ttttgacctt atatcgcgtc
     3421 cccgattcct atttttccac tcccgataca gtcacgccca aatactcctt tttctttata
     3481 tactccacgt ctactcgatt cccgggaaca gggcagacag gagagtatca gcaatggcag
     3541 attttgtcga gaaaagcgca gtcaagtccg caacccgtga gcttgcagct ccga
//
LOCUS       DAJB01000234            4275 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_10763, whole genome shotgun sequence.
ACCESSION   DAJB01000234 DAJB01000000
VERSION     DAJB01000234.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_10763"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          593 a         1446 c         1370 g          864 t
ORIGIN      
        1 aacagttgtt tcgtagggca ctaccgcctg ctccacccgg gccgcgtgct cctcgatgga
       61 ggggacaccg acgaagaccg ttatcggctc gccagggtag gtccgtggga ggatctcgcc
      121 gcctatccca cccgtcgtta tctcgaaggc gccctccatt cccacaaaag gctccgtcgt
      181 ccaggcgaag acttcctcgt agaacgcccg cgcccggtcg gtctcgccgg cggggacgtt
      241 gaaccagaca atcgtcggca tatgcatatc tccggccccc ggtgggtgcc acggcgaata
      301 attgtttccc ctgcccggct aaaagatatt tatagccggg cacggataag atggtccatg
      361 gcggcactgg gcaggcgcag gttcggaaag acgggagacg aggtcacccc cgtggggctc
      421 gggggcgagg gggtcctcag gacgtacggg cggcacgcgg aggcaaacgc cgtcatcatg
      481 gaagccctca atcaggggat cgcctacttc gactcggcga aagcctacgc ggggagcgaa
      541 gactactacg gcgaggtctg ggtgagtcat cccgacctcc gggcacccgt attccaggcc
      601 agcaagtcgg cgagccggca ccacgcggat gctgagatgg acctccagga taccctccgg
      661 aggatgggca tcgagaccct cgacctctgg cagatccacg acgtccggac cttccccgac
      721 atnnatcggg gtgacgggac accacgaccc ggacgtcctc tcgcatgcgg tggagaactg
      781 gccggtcgac gccgtgatga tgcccgtcaa cccggtggag ggggcactcg ggggtttcct
      841 ctcctcgacg ctccccgcgg cgagggagca ggggctcgcg gtcatcggga tgaaggtgct
      901 cggcgggtcg aactaccttg ttcccgacgc gggggtgacg ccggaggccc ttgtccgcta
      961 tgccctcgcc caggatatct ccgtcgcgat cgtcggctgc tcgacgcccg cggaggtgca
     1021 ggcgcttgcc gccgccggac ggcgtggtcc catgcccgac gacgaagcgg cggcgcttgt
     1081 cgaagcgttc cgcccctatg ccagggagct cgcgtactac cggggacccc ggtgaagcag
     1141 ccgctggcag aaccactctc ctgtaaccga tatatgctga cggcgccaac ctacggggag
     1201 agagcagtgc cacggggacg ttgacgtgcc gcagggagac gaatcaccat gacaaccagg
     1261 gagccgcatc ggttccacac gttccgggac cgcatcgccg catcgccgga cgcactcctc
     1321 gtgctggtcg tgctcgtcat cttcatggac atgatgatct acggcctcct cattccggtc
     1381 ttcccgcagt atgcaccccg gctcggggtg gacgaatcgg tcatcggggt cgtcttcggg
     1441 atttacgccg ccatgctcct cctcttctcc atcccgatgg gcctcctctc cgaccgggtg
     1501 ggccgccgtc ccctcatcgt cgtcgggatg ctcctccttg ctctggcgac ggtctttttc
     1561 ggcttctcga caacggtcac gcacctgatc gttgcccgga cagttcaggg gatatcggcc
     1621 gcggccacct ggtccgcggg gcttgcgctc ctcgccgaca cctgcgatcc cgccaggctc
     1681 ggggagaaga tgggcgtcgc gctctcggcg gtcgggctcg ggaccgtcct cgggccggtc
     1741 gtcggggggc tgctcttcga gtacgtgggc tacaccgcga cttttctcgt cccggcggtg
     1801 ctggtggcct cggttggcct ggttgtcctc gccgtcccgg tgcgcacctg caggcaggag
     1861 ggcggtccgg ggcggtcagc gatgctgccc cgcggaaccc tcctcccgct cgccgcctgt
     1921 gcggccgcga tcattatggt ctccgggacc tatggtgtcg tggaccccta cctcccggta
     1981 tacctgcacg ccgtcttttc gacgtctccg gcattgatcg gcctcgtctt tgcggtgctg
     2041 gcggtcgccg ccgccatcgc gcagccggcc gcaggaagga tttacgaccg gcacgggggc
     2101 agccgttacc tcatcggcgg cgggctcctc ctctcggggg tcgcgatcgt cgccgccatg
     2161 caggcaccga ccctcgtgct cctcattgcc gccatctttg cgctgggggt cacgctgagt
     2221 tgcgccctgg tgccggcgat gccgatcctc gccgacatct accggggcca gggctcgcag
     2281 ggtgccgcct acggcatgta caacactttt ttctccgtag gactctcggc cgggccgttc
     2341 gcgggagcca tcctcgcggg ccggtggccg ctgccggtga tattcctgct gcaggcggtg
     2401 gtcctcgcgg ttatgggggc tctgggctgg cttgtcatcg ggaggctcgg gtggcggtga
     2461 gggtgcgcta cgttcatcgt gccgtcgggt caacactgtc tcctgccatc gaaccgagga
     2521 taccatgtcg ctggaattct gccgccgcca cgcccccgaa ctgctgatcg ccttcttcgg
     2581 tctcatcttc cttgtcgtga tctccggggc cgtcggcggg gaggcggggg cgttcctcac
     2641 ctccgcgctc aatgtggcgt tcttcgtcgt cctcgcgatg ctcgtctacc ttgccgggga
     2701 ccggcaccct gcgttccggt gggctgccgg cgcctggctc ctcgtccttg tcgccgggct
     2761 cgcgttcacc gcggccggga tgggagttat agccatcatg ccggcggagg tgctcgaggg
     2821 cggggagttc gagacgttcg accttgcgct ggctgcggag gtggcccttc tcctcctcgg
     2881 cgtctctatc gcggggttcg caagcctcat cgggctctcc cgctggttca ggggctggct
     2941 cgcccgttac ctcccgttcg acccggactc gctcctccat acggttgcgc ttgtggtcat
     3001 cctcgccatg atcctcatcc caccggtgcc gctcctggtg accgggggtc cgcccttcct
     3061 ctcgccgcag ttccttgacc tcctgctgga gtccggggac ctcctcgcgg atacggtcac
     3121 gttgaacgcc tacaccctct tctggacgct cataggctcg ttcttcattg cgggggcatg
     3181 catccggcgg acgccccgcc agaccctgga gcggctcgga ctcgtccggc cgacggggaa
     3241 ggaagtcgct ctcgccgttg ccgcggctct cgcgctcgtc ctggtgttct ccttcatcga
     3301 cccggcgctc tccgcgcttg tggactggct cggcctccct gtcaccgacg aggatgccgt
     3361 caacctgctc tttgcaggat cgctcaccct gcccgggatc atcgtggcgt ccatcgcggc
     3421 gggtttcggc gaggaggtgg gcatccgcgg cctgctccag ccccggttcg gcatcctcct
     3481 ccccgcgctc cttttcgcat cgctccacgc gttccagtac agctgggacg gcctcatctc
     3541 ggtctttctt gcggggcttg tctttgccta catccgcagg tactcgaata ctacgacgtc
     3601 ggcgatcacg catacggtct acgacctggt gctcttttcc ctgctcctga cagggatgtc
     3661 gatctgatgc gggtgtccca gtttttcaaa atagtgtgcg gcgccggagc cgcgatcgtg
     3721 cgtattgtgt cttactccaa cagtcccctg cgccgtatcg cctgcagcgc ggcgcgtgcc
     3781 tcctccaggg tgtacgggcg gccggccctg atatcttcga gggactcctc gattccccgg
     3841 atggcctcgt cgctcagggg ctcttcgtcg tgaggcatga tactgtctgg aagcctatca
     3901 gatatcaagc tcatggtagt gaggatggga aaataagaaa cgctgagggg gagatttgaa
     3961 ctcccgaggg gctcaacgcc ccacgggctt tccaggcccg cgccttaccg gtctagacta
     4021 cctcagcaat gtgcatcatt agttggcggc gttgtctatt aataatatct cccggtgggc
     4081 gtgccgcccc ggagccgggg agaatatcac gagcgtcctg acgccccgga ttctctgacg
     4141 tgcgccttcc agcccctggg gtaggtgccg ggctgcatca tgacggcagt gggggcgatg
     4201 acgaacccgg gctcccccgg ttttaacgcc gacgaattga tgagggcctg cccgagcgcg
     4261 acaagctccc cacgc
//
LOCUS       DAJB01000235            3032 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_22196, whole genome shotgun sequence.
ACCESSION   DAJB01000235 DAJB01000000
VERSION     DAJB01000235.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_22196"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2782..2864
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          620 a          925 c          925 g          479 t
ORIGIN      
        1 ctctacgcga aggcgttcga gttcatctgc ctctctgagg ccgaaaaccc cgccaaaatc
       61 cgggcagtga agatgctcgc ccggacctgc accgagatct gcgaggggca gagcatggat
      121 atggcgttcg ggacgaggga cgacgtctcg gatctcgatt accttgagat ggtcagcaag
      181 aagaccggcg tgctctacgg cgcgtctgcc gccatcggcg gaatccttgc gggtgcaaac
      241 ccggtccagg ccgatgccct ctaccagttc ggggtgaaca gcggcgttgc cttccagata
      301 caggacgacc tcatcgacct ccttgcaagc accgagaaga gcggcaagga ccgcgcatcg
      361 gatatccggg aagggaagca gaccctgata gcaatcaagg cacgcgagaa ggggctcgat
      421 ctcaccccgt accgcagaga actctcggct gccgagatcg atgacctgat cgcccggctg
      481 gagggtgcag gcatcatcga agaagttcgg gccacggccg tcgagcgggc gaccgtcgca
      541 aaacaggcac tctcgatcct cccggactca gaggagaaac agcttcttgt cgagatcgcc
      601 gattacttca tagaccgggg ttactgacac catggacacc gatatcgagc atctgctctt
      661 catctacgcc ctgcagaacg cggtgaagca cgacgggatc ccaaaaagcg ggacggtcat
      721 cggcacggtg ctcggcaagc atcccgagtt ccggagccgt gcccgggaga taggagcgct
      781 cgcggggaag gctatcgcgg aggtggcggc gatgagccag gccgaccgga agagccgtct
      841 cttagagatc gcccccgaac tcctcgacga actcaccgag acgcacgagc actcaaggga
      901 actccctgcc ctcgagggcg ccgagaacgg cgtggtgatg cggtttgcac ccaaccccag
      961 cggtccgctg cacctcgggc atgcccgggc ctccatctta aacgactact atgtccgacg
     1021 ctatgggggc cggtatgtcc tccgtatcga ggataccgat ccaaggaggg tcgatcccga
     1081 cgcatatgca atggtgcagg aggatatcgc ctggctgggg ctcgggatca ccgatatcgt
     1141 ctaccagagc gacaggctcg atatctacta cgactggtgc aggaaactca tcgagctcgg
     1201 cggcgcctac gtctgtgtct gtgatgcaga gcgcttcagg gaactcaaac tcaaggggaa
     1261 ggcctgcccc tgccgggagc ggccggtcga ggagaacctg gaactctggc agcagatgct
     1321 tgacggagag ttctacgagg gggacgtgac cgtccgggtg aagacggaac tcacccaccc
     1381 cgacccggcg atgcgggact actccgcgat gcggatcgtg aacgcgcccc tccacccgag
     1441 agtggacaac acggtctttc cgctgatgaa cttctcggtc gcggtggacg atcacctgct
     1501 cgggatcacc cacgtgatcc gcggcaaaga ccatatcgcg aatacgcgga gacagcgctt
     1561 catcttcgac tacttcggct ggaagccgcc gttctaccgc cactacggcc ggatgggaat
     1621 atcgggcgtc gtcctctcga catcggggat gcgcgagggg atcaacaacg gcacctacac
     1681 cggctgggac gatattcacc tcgggacgat gcaggcaatc gcacgccggg gtatcgagcc
     1741 cgaggcggtc cggaacgcca tgatcgatat cggcgccggg gagaccgaca tatcgttctc
     1801 gtgggagaac ctttactccc ggaacaagga gattgtcgac ccgaaggcga accgctactt
     1861 ctttgtgccc gacccggtgg aggtcaccgt ggagggcgct cctctccacg aggcccacgc
     1921 ggccctgcac ccgaacgacc cctcccgggg ggtccgcacc ctcgtcaccg ccggaagggt
     1981 gctcctcccg aaagcggatc tcgagggccg gagcatgatc cggttaaaag acctctacaa
     2041 tatcaggatc gcgtggaatg gggatgaacc gcacgtatcc tacgcgggcg acgcactcga
     2101 ggatgcccgg cgcgagaagg cgccgatcgt ccagtggctg ccggcggacg cgaaactccc
     2161 ctgcaccctc ctccggcagg acggaagtct cgaagggttc tgcgaacccc tggtcgccgg
     2221 agaagtggac cgtgtcgtcc agttcgagcg gatcgggttt gcgcggatcg actcggcgga
     2281 caacggccgg gtgagcgcgt attttgcgca ccggtgaagc ggctcccgca gatcacccgg
     2341 ggaagagacg gctgaggcaa cgccgcccct gcccctcccc ggcgaaccgg ggcggacccg
     2401 gtccggcacc cactcgtttt cactgccggt cgggagaaaa ggacgccatc cgcgaaggta
     2461 ccgcaaagcc ggagcaaccg gatctttcgg ttcggccact ggagcggatc gccgcacggc
     2521 tgctcccgta cgaatcccgg gatagagcag atccccttcc ggcctgcgcc agggggtatg
     2581 cctgagcctg tttcggagtt tttctgtttt acggcaggtc tgcagctgca ggcccgatct
     2641 tcccggtcgc caaccgccgt tacgagagcg ggcactgcaa atctccaccc catatgtaac
     2701 attacaataa ccaatagtaa cctattaata acaaaaagta taatgtattt tacgtaagga
     2761 gcaggtcccg ccgccccacg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaaccg cgatcagggc
     2881 cgccgtcgaa cagggggatt acgagaccgc acggacgctc atgcaacagt tcagggaaga
     2941 gcaccggatc gagcgctccg ccccgccggc cgacggcaac cgggagatgc ggatcgcagg
     3001 gctccttgac cggctcgaag aacagggcgt cg
//
LOCUS       DAJB01000236            4969 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_19515, whole genome shotgun sequence.
ACCESSION   DAJB01000236 DAJB01000000
VERSION     DAJB01000236.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_19515"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1411..1543
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4412..4439
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1016 a         1438 c         1491 g          863 t
ORIGIN      
        1 tcgacatcga cccggtccgg ccgagcgggg tctcgtcgac cgacgccgag cacgacgcgg
       61 cgctcgccgc ggcggagcgg atcgcggcct acctcgccga gcaggggttc ggggcgcccg
      121 tccgggccga ctcgggcaac ggcgcccacc tcctctaccg ggtggacctt ccaaatgatg
      181 agccctcgac cgcgctcgtg aagggctgcc tttcgaccct cgatgccctc ttctcgaacg
      241 aggccgtgac cgtcgatacc gcgaaccata acgccgcccg gatctggaaa ctctacggaa
      301 ccctgtcacg gaagggtgac aataccccgg agcggcccca ccggcgggcg aggctgctcg
      361 cggctccagc ggagatcggt gttgttcccc tggagcagtt gcagcatctc gccgggctcc
      421 tcccccggga agagcctcct cagagaaaaa acggccccgg tatcgacctt gccgcctggc
      481 tcctcgacca cggcatcgcc gtccggtcga cgcggcccta tctgggcggc acgctctaca
      541 tcctcgagga ctgcccgttc tcgtcggccc acaaagacgg ggccttcgcg atccagtttg
      601 caaacggcgc cgtctttgcc ggctgccacc atgcaagttg cggtggaggg gcgcagcgct
      661 ggccggaact ccgggagatg tacgagcccc gggagaagca gaagaaaaag cgggaggagg
      721 cacccgcccc tcccccggca ccagtggagg acgagcaccg ccgcagggcg acggagatcc
      781 tccgggacgg cgacccgctc gccttcctcc tcgacacctt ccacaaagag cacgtcggcg
      841 accggaccgt cgcggagtgc ctggcgatgt ccatcgcttc acagtctgtc gcgaacacga
      901 aggggctgca cgtcgccatc tccggcaact ccggcaaggg gaagagccac gcctgcgtaa
      961 cgatgctcaa cctcatcccg gcggactaca aactcaccgg cacggtgagc aacaaggcgc
     1021 tctactacgc cgacgacctc cggccgggga cggtgatctt ctttgacgac acatcgctct
     1081 cagacgacct gcaggaggtc ctgaagtcgg caacctcgtc cttccacgaa ccgatcgagc
     1141 accggaccgt cacggccgac cggcaactcc gggtctgcac catcccggcc cggtgcgtct
     1201 ggtggctcgc gaaggtcgag aaccccggcg acgaccaggt gatgaaccgg atgctgacgg
     1261 tctggatcga tgactcggcc gagcaggacc gggcggtgct cgagcacctc aaggagacgg
     1321 aggcgagcag acctgccagg ggcgacgacc cggacgtcct caccgcccgg gcgctctggt
     1381 cgatcgtgaa gcagcagcgg atcttcgtcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctgcga tcaatcagga
     1561 gggcggcggg caggactcaa agatgacccg gaacgaagcc gcggcgctcg ccaccatcgc
     1621 ccggatgggc tgggagtcgt tcaccgtccg gatgctgcag gaggcgacgg ggctctccta
     1681 ccaccaggtc cggcggatcc tgcagggcta caccgcgagg ggcatgacct actgcggcct
     1741 cctgaagaag tgtcctgcca tcagcgtggt ggacgcgacg gtcatcgacg agagcaccgg
     1801 gacgaccgtc cgccggcatg aactccattt ccagttcgat gccgggcgct accaggagtg
     1861 ggtggcagag ctcgcggtct ggctggagga tgagggcgag ggggaggatg actccccacc
     1921 gccttctagt gaccatttgc aacatctcca accctgttgc aaaggggtgt gcaaacatga
     1981 gtgcaaagag atcgagggtg ggagttctca attgggtgag ggggatttga tgaacaaaga
     2041 gatactcgca gagatcaatc acggcgttca gggatcgttt gcaacaaatt ccagatcaca
     2101 cagttcacct gatcctgctc cactggaaga gcaccgtgat cgcatgcacg accggggtgt
     2161 gccgtgtgtt cctggacctg ttgcaaacga aacgatcgaa catgatgatt tgacggtgaa
     2221 tcagaaaaaa ctcaacgcat tgagtggttc gagcgtgaaa agtgtttgca ctcctatttg
     2281 cacccccctt tgcaacacgg gtgcaactgt tgcaaactat gtccggccgc tctccggggt
     2341 gcttgacccg aggacgtttg cccgggtgaa ggtcgaactc ggccggtgcg atatctgtga
     2401 tacgggaaaa gcggtctacc gttcacgcga ggcacagacg catatctgtg aggggtgcta
     2461 tgcccggctg gtgcgggagg ggaacgcgag ggagggggtg aggtgatccc ctggacagtg
     2521 aaagatggcg tgcaccaccg cagagacttt tgaggcatac ctccctattt ccggattctc
     2581 ctcgtttagc agggctttaa aaaccgaaat cataatccca tcccgcaaac aaaggaacct
     2641 tcagcagaag gatagcatta ccattgctct gccgttcatc ctggagaaaa cacggtagtt
     2701 tcgcccttta tccgatcaga cgtgcagaag aacgcacaat cggagagata ttccgccatg
     2761 ctgcaaaagg aagatatcag aagagatgcg ctcggccgga tcgtgcgcat cactatgaac
     2821 gcgctgccgg gctacaccag gcctgccgac cgacggtgaa gtgatggggc gtcatacatg
     2881 agagaagcac atgatgtttt tgctcgggta cggtgctgga agtgaagcgc gtggatgacc
     2941 tatactccct gctcgcccgc tttgaaggac ggcggatcga tttcaaggaa gaagtgagcc
     3001 cgaacctcta caggcttctg tccgcgtttg ccaacaccgc cggcgggata gccgtcatcg
     3061 ggatccgcga ccatgatcat gccgtagtcg gagtcgacct cagaaataat gccgtcaaaa
     3121 aactcgcaga cagcataacc acccggcttg gcattcatcc ggtcatcgag atccgcgaga
     3181 tcgaggggaa gtccgtcgtc accgttgccg tcgaacggag ccgcgtgccg gtcgcctgtg
     3241 acggccggta ctacacccgc atcggcgaca cgacacgcga gatgctcccg gacgagcttc
     3301 gcgggttctt ccagcagagc attgagtggg acagcgtcgc ggggccgtac tcgttcgatg
     3361 agatcgacga ggagacggtg cggcgtttcc tcgccggtgc aaaggcggca ggtcgcctcg
     3421 ctgccgtcga tccggccgaa ccggtcgagg ccgtccttcg ccgcctcggg cttgccgggg
     3481 acggagggat cagcaacgga gccatcgtcc tcttcggcag agacccgcaa cggtacttcc
     3541 agaactgcat tctccggatc gggcggttca agcgggcgga tatcatcgtc ggcgaccacg
     3601 agatcagggg caatctcttt caccagtttg aagagggcga gcggattatc aagcagtatc
     3661 tcggcgtcag gtacgacatc tcggaggagg cgatgcagga gtcgtttcag aggagagagg
     3721 tctgggacta cccgcttccg gcgatccggg aggcgcttct caacgcgctc atccaccgcg
     3781 attacttcaa caacacgatt cagacgcagg tcaagatctt tgacgaccat attcgcttcc
     3841 acaatcccgg ccatctcccc gaaggcgtta ccatcgatat gatcttaaaa gagcactact
     3901 cctaccaccg caacccgaag gttgccgata ttttctaccg tgccgggctc gtcgaacgct
     3961 acggatcggg gatcgagcgg atcatcacgg ccctccgcag cgccgggatg ccggttccca
     4021 ccttcgccag cagtccgctt gggttcaccc tgacgatgcg gatggatatg ctgaacgaga
     4081 gttcccttcg gaagatggaa ctgaatgaac ggcagatcct tgcggtggaa cgggtcaagg
     4141 tcgaggggag catcacgagc ggtgcctatc aatcactcac cggtgtctcg gcagcgacgg
     4201 ctttcaggga tctcaaggcc ctggttgacc gtggtatcct ggagcaggtg agcccgtcga
     4261 ggaggaatac acgatatgtc ctgcgggaaa ggccatcatg aagtgtggat atttccaaag
     4321 cgttccggcg aaacaggggc tggtccgggc ctgaaaccgg gtagatgccc ccctggcgtg
     4381 ggtaaatgat ggatggatga gagatggatg annnnnnnnn nnnnnnnnnn nnnnnnnnna
     4441 aatcgtgatt tgtcgattaa tcggcaatcc ccaagtgcag cggagagttc acagacaaaa
     4501 agtgtttgca cacccctttg caacaccatg tacaacagtg ttgcaacacc cgtccggccg
     4561 ctgcccgggc tgctcgacca ccggatgttt gcgtgggtga aggagatctc ggggggtgta
     4621 cgatctgtga taccgggaag gcggtctacc actcccgcga agtacgggcg agtgtctgcc
     4681 aggggtggta tgcacggctt gtgcgggagg ggaacgcgaa ggagggagtg cggtgatccc
     4741 cgacgctccg accattcatg agctcggagt cctcccaacg ccaggtgtcc gatacgtccg
     4801 aaaaatgtcc gaaaaatgtc cgttagtcga ataacaaggt gggattactc ccaccccgtc
     4861 ttccaggaac ccaccttgcg actttcaccg cagtaggctc gcgcattcct ctacccattg
     4921 ctgggcagca acgcaaaata ggataatgta gtttgaatcc atggtgttt
//
LOCUS       DAJB01000237            2624 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_64877, whole genome shotgun sequence.
ACCESSION   DAJB01000237 DAJB01000000
VERSION     DAJB01000237.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_64877"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    1943..1962
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          802 c          853 g          484 t
ORIGIN      
        1 gagtggtcac tccagctcga ccgggaggcc gaccccgagg ccgtcccgat ccgccccccc
       61 tatatcatga aagtcctctc cgagaccctg ccgggattgc agcggggcca tgagacccga
      121 tgaagtcgga ggtccggacg ggacaggtct ctggcaggcg gcaggggggg cgtggcacac
      181 ccctcccgaa atgtataagt attttccatg ggaatgaggt cccgagggta tatgtgaaca
      241 tcccggtgcg ggtgcataac cagtggcgcc cataccgggt acgcgcggat atccggatgt
      301 cgatccgggt gggagcatcg gtttctcgtt gctgctccac gctaaagggt tcccgggtgc
      361 ccgtccgttt gacgggcgtt ccgggcagac ttgcggcgaa ccggatccgt tccggttcac
      421 cgctcttgcc ggcaaacggc ggagtttttt cggaccacgg cagatagacc tggctctctt
      481 ccggcggaag catgaaaaag agtggaattg gattaatcgc cggagaggta gtcgtagagg
      541 aacgcagcga gcaccgcgcc gacgatgggg ccgatgatgt agatggggaa gaacgcccag
      601 aggttctgcc cgcccaggag ccagtccccg aggtacggcc cgaacgtgcg ggcggggttt
      661 aacgacgcgc cggtcaggtt cccggtcgtc gtgatgatgc cggcaaccgc aagacccacc
      721 gcgaggccgg caaagcccgg cgtggcccgc tcgtcgacgg cagcgcccat gatcacgagc
      781 atcaggagga acgtcccgat ggcctcgatg acgatcgcct gcaggtagcc gatgccgagg
      841 aagggtgccg ttgcgccgag gcccccgacg gtgacggcgt ccgggccgac cgcccaggca
      901 aagagcaggc ttgcggcggc tgcgccgagg agttgtgcga cgatgtaggg gcccacgtcg
      961 cgtccgggga accgcccggt cgcaaagagg gctatcgtca ccgccgggtt gatgtggcat
     1021 cccgatatcc ttccgagggc gtagatggac ccggcgatgg cgatgccgaa ggcgagtcct
     1081 atggcgagcc agtcggcgag acccccgagc accccgatcc cgatgttgaa gggagtcgac
     1141 ggggtggtcc ccgaggcgag catcagcgtg acggctgctg caccggcccc gaagaagacc
     1201 aggatgaagg tgccgacggc ttcggcgacg aaccgctttc caggagatac agtcattgtt
     1261 tcaccagata atcatatcat ctttttagag taaggctttt gctaaaattg tatgggagag
     1321 tgccttattt cccgtacagg gcggtgtagc attcggggca gaggatcctc ggcagcggag
     1381 ccttcacgtg cttgctgggg agcgggtagc ccccttccca gacatcgata tcctttctga
     1441 tggcgtgctc catgcagagc cccattccgc agacgacgca gactgccacc gcctcgctat
     1501 cccccccttc aagggcgcag tagtagcact tcatagcaac tctccctcag accgcctctt
     1561 tccactggca gtatcgctgc cgctggtcga ccaggcatgt cgtggcgcag ctcttgcagg
     1621 cccgcaccgg agcggcggcc gtctctttct caagggcgat gacgttggtc atcatcgtcg
     1681 cggtcacggg ttcgctcgtg aggaggcacg ggtagtcggc aaggcgcatc agtgcggaga
     1741 accggaccgt tatggcgcag gccgggtagg cataccactg cgggttcatc agcacctcgt
     1801 ttgcgtggat gggcgagaga tcgacggggt acttgccgac catgggagtc agggcctcgt
     1861 cggccccgtt ccggttccgc cgcgccttat cgaggagttt ccatccgcgg tagatcatgt
     1921 ccatgaagtc gcagttgtgg agnnnnnnnn nnnnnnnnnn nngagtcgag cgagtttcgt
     1981 agcgtcgact ccatcacctc ggtgaccgcc tcgatcaccg ccatgacgac gtcgtccttg
     2041 cacatgttga aggtcgactg cgcgatgtgg tgcgcaacgt cgccgacgct gtaggccgga
     2101 acggtcagga tgttggcgta atggacgccg tcatcgactg cggcgcgaac agtcccttcc
     2161 atccgccgcc ggtactcgct catgtacttc cgcggatcaa aagaggtcat cccggcggca
     2221 tccatcagct ccgcctgggc ttcgaccggt tcgcggtaga tcctctgcat catctcgatc
     2281 tccctcgccg tcgcctcggc gggggtggct cctgcctcaa gggcgtgggc gaaggtgtcc
     2341 ccgaagccgt aggaggtgtt cattccccat gatttggcgg cgaggatcgc ccgcttgtgc
     2401 tcgaccggga tgtcggtctt ctgtaagacc cggttcacca cgttgctcgt gctcccgggg
     2461 atcagcgcaa agtcgacgac acaggtgggg ccgtagaacc cgccgtagcg ccgcaccgcc
     2521 tcgaggccga tccgggcctc cgcatccgcg actgccttcg tgaaggcatc gacgctcttt
     2581 gcgaacgcct cgtcctgact cttcaagatc tcgagcacca cggg
//
LOCUS       DAJB01000238            4583 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_116986, whole genome shotgun sequence.
ACCESSION   DAJB01000238 DAJB01000000
VERSION     DAJB01000238.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_116986"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2159..2184
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          879 a         1494 c         1461 g          723 t
ORIGIN      
        1 accccgccgc atatcgtaga ggctctctgc gccgcggccc gcgacgaaaa gaaccaccac
       61 tacccctcct acaccggcat gcttgcctac cgcgaggcgg tggccgactg gtaccggggc
      121 aggttcgggg tcgatctcga cccgaagaag gagacactcg cgctcatcgg gtccaaggaa
      181 ggcatcgcgc acatcgccga ggccttcgtc aaccccggcg agatcgtcct cgctgccgat
      241 ccgggatacc ccgtctacaa gacgtccacg ctctttgccg aaggaaaggt ccacgaactg
      301 cccatccggg cggagaacga cttcctcccg gtgctcgagg atatccctgc cgacgtggtg
      361 aagcaggcaa aactgatctt catcaactac ccaaacaacc ccactgcggc gatagcgccc
      421 ctctcgttct ttgaggaggt cgtcgagttc gcgcgggagc acaacatcgt cgtcgtccac
      481 gacaacgcct actccgagat caccttcgac ggctaccggg caccctcgtt cctcgaggct
      541 gacggcgcga tggaggtcgg ggtcgagatg cactcgctct caaagaccta caacatgacc
      601 ggatggcgga tcggcatggc ctgcgggaac cccgggatca tcgccgggct cggccgggtc
      661 aagaccaacg tcgactcggg cgcgttcgac gccatccagc acgccgcgat cgccgcgctc
      721 accggccccc aggactgcat acaggaggcc tgctcggtct accaggagcg ccgggacgtg
      781 ctcgtgaaag ggctctcgga gatcggcttt gatgtccagg cgccgaaagc caccttctac
      841 gtctgggtgc cggtcgacga ctgcatggcg ttctccgcaa aactcctcga tgcggccggg
      901 atcgtcgcga ctcccggact cgggttcggc agcaacggcg aggggttcgt ccggttcgcc
      961 ataacccggt cgatcgagcg gatcaacgag gcggtcgacc gtatgcgggg gctcgacctg
     1021 tgatcctccc ctcccacctc tcggtcaggg acggccacct ccggatcggc gagcacgata
     1081 cggtggacct tgccggacgg ttcggcaccc cgctctacgt caccgatgag atgcggatcc
     1141 gcgataactt ccgccgtctc tccggcgcgc tcaccgccca ctacccgaag gtcagcatac
     1201 tctacgccgc aaaggcgaac ggcaatctgg cggtctttcg gacgctcgcg tcgatgggcg
     1261 ccggggccga tgtcttctcg gccggcgagg tcgcgctcgc ccttgcggcg ggcatgcgcc
     1321 cggtttcgct cctcttcaac gggagttcca agacccccgc cgacctcgcc ctcgccgtag
     1381 agaagggcat ccgggtctcg gtggactcgc ccgacgagct ccggcagctc gaggcggcag
     1441 ccgcgaaggc caacaagacc gtcgatatcg ccttccgggt caaccccgcg atcgaagccc
     1501 ccacccaccc aaagattgcc acaggcctcg ccacgagtaa gttcggcatc ccggcgggag
     1561 agatcctcga tgcctaccgg gaggcgctcg cgctcgaaca tatcaacccg atcgggatcc
     1621 actgccacat cggctcgcag atccttgccg tggaaccctt cgcccgcgag gtcgaggtgc
     1681 tgatcgatgt tgcccgcgac ctcatcggta tcggggtcga cgtgaaattc atcgatatcg
     1741 ggggaggcct cggcatcccc taccgccggg agaccgaccg ggccccgacg ccggaggagt
     1801 atgcgggagc ggtcatgccg gtcttcctga agggtatccg ggacctcggg atcgagcccg
     1861 aactctgggt cgagccgggc aggtggctcg tcgccgactc gacgatcctc ttaaccaggg
     1921 tcaactcggt caagaccgct catgcgaact tcgcgaacgt cgacgccggg ttcaacctcc
     1981 ttgtgcggcc gacgatgtac gactcctacc acgaagtcgt ggcggcagac agggcggacg
     2041 cacccgcggt gcgggaatat tccgtcacgg ggccgatctg cgagacgggc gacatccttg
     2101 caaagggccg gatgctcccc gatctatcgg ccggcaacct catcgcggtg ctggatgcnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnngagacg atcgacgacc tgaccgcccc catggtcact
     2221 ccgccctggc aggcataagg agcggagcgg cagcgtgaag ttccactatc ttcttgtcga
     2281 tgacctcctc gaaaaggggg agttcgagcg ccgggtggag gagaaggtag cggagtcagg
     2341 ggacctgctc gacgaccgga cggcggcgat gctggtcgtc aaagacctcg ggcgggcgca
     2401 tatccggata cacgacctcc cggccgcccc gagcctcgcc tgcttctttg caaaggtgct
     2461 ctccgtcgag gagccccggg aattcgagcg gcccgacggc acgaccggcc tcgtcgcaaa
     2521 cgtgacggtg ggcgacgaga ccggcagggc caggctgacc ctctgggacg agaaagccgg
     2581 cgccgtccac gagatcgagg caggggacgt cctcgagatc ctcggccggc cgaagggcaa
     2641 aggaaaggtc cccgacgtca ccgccgtcgc cgtcgagaaa gcagcgtgcg agatcacctg
     2701 cgacgataca ggagcccccg cgcttgggcc gggaggcgac ctcgaagtca ggctcattgc
     2761 catcgagagc ccgcggacgt tcaagaagcg cgacggcggc accggggaga tggttgaagc
     2821 ggtgatcggc aacgaagacg gtgtcttccg gcttgtcgcc tggacccccg cagtcctcga
     2881 agggttggaa gccgggggaa acgtcgtcat ccgcggcgcc accgcccggg agggcgagca
     2941 gggcatcgag tacagcctcg gcgatgcggc gtccgtctcg cgctccgacc gggagatcag
     3001 cctcacgctc gataccgtcg cggatgtcgt ggaagggaga tcgtattcgc ttgccggaac
     3061 ggtggcaggc gtgcagccct ctcacgcgtt tgtcacgcgg agcgggaggc agtcctgtgt
     3121 ccgaaacctg gtcctcgccg acgagacggg ggaggttccg gtcgttatct ggggcgagaa
     3181 ggcggacggg cacctggtag ccggcgaccg gatcgagatc tataacgcaa ctgcccggcg
     3241 ggggcgatac ggcgacatcg aggtgcatct ctcgtggggg agcgctctcg ttctcctcgc
     3301 acaggaggag aaggaggtgg aggtcgaagg gacgatcatc gccacccggg aggggatcgc
     3361 tcttgatacc ggggaagcct gctacctcct cgccgagccg ctgcccatcg gatctattgt
     3421 gcgcgcccgg ggccgggtgc ataggggtgt gatcagtcca ggcgacatcg aggccgtaac
     3481 gcccgatccc gaggatctgc agcgtcgcct ggatcagttc tccgggagac cctgatcccg
     3541 atcttcactt tcgccccgca cggcgagcga cctttatggt tcccgctata gactcttagt
     3601 gtaagcagag cagacacacc atagagatca agtgaggatt gagatgacag agattgatct
     3661 tgaagattta cccggcgtcg gatccaccac cgccgataag ctccgcgagg ccggatacgg
     3721 caccgtcgag agcattgcaa cggccaccac gtcggatctc gctgaagcgg ctgagatcgg
     3781 cgaggggacc gcaaagaaag tgatcctggc cgcccggaag atggcggata tcggggggtt
     3841 caagacgggc cgcgatatcc tggacaagag aaaggatatc aagaagctga agacgctggt
     3901 ccccgagttc gacgaactgg tgggcggcgg acttgagacc caggcgataa cagaggtcta
     3961 cggcgagttc gggtcaggga agagccagct cgtccaccag atggccgtca acgcccagct
     4021 ccccatagaa cttggcggtc tgcacgggtg cgtcctctac gtcgataccg agaacacgtt
     4081 ccgcccggag cgaatcgagc agatggtcaa cgggctcccc gaagaggcgg acatcggccc
     4141 gctcgaggat atcctcgagc ggatccacgt cgcgagagca cacagctccg accaccagat
     4201 gctgctcctt gagaccgcac gagaactggc aaacgacctg cggacctccg attacccggt
     4261 aaagctcttc gtcatcgact cgttgacgtc cctcttccgc tcggagtacg cagggcgcgg
     4321 cacgctcgcc gcccggcagc agaaactgaa tcgccacatg catgacctcc tgaaactgat
     4381 cgacgaccac aacgccgtcg gtctcgtgac caaccaggtg atgtcaaacc ctgccgtcct
     4441 cttcggcgac cccacgaaac cgatcggcgg caacatcgtc gggcacaccg caaccttccg
     4501 gctctacctc aggaagagca agggcggcaa gagggttgcc cgcctggtag acagccctaa
     4561 cctccccgag ggcgaggcgg cat
//
LOCUS       DAJB01000239            2585 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_134328, whole genome shotgun sequence.
ACCESSION   DAJB01000239 DAJB01000000
VERSION     DAJB01000239.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_134328"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          481 a          784 c          764 g          556 t
ORIGIN      
        1 gatgcgaagg gcgggaaagg aaattcatag agttaaatga caacgagtag tagagttcat
       61 gctctgcgtc ctccccgaat gcgaggatgt atgccccgac ggtccggctc ccggccacct
      121 caagctccgt aaactcccac ttatctccgg ggttctccgg cggcgttgca gcgaagatga
      181 tgccgtcgcc ctcagcaccc gcccggttcc attcgccgaa gatgtagcgt ccggcgagcg
      241 cagggatggc gctgccgcgg tagacgtatc cgccgatgac gacctcgccg atgccgccgg
      301 gctggccggc gttcgggtac tcgatgatcg cgggaacgag aggctcgccc cgccggccgg
      361 tccggggcac gtcttgtggc gactcgtagg ggttagtggg gtcgaaggca tggttgcctt
      421 ccttaaggtt ccacccgtgg ttgccgcccg gctcgatgat ctttaccgac tcccagaggt
      481 gctgtccggc atcgaaggtc gtgcgccacg ggttgcgcaa cccgtaggcg tagatctcgt
      541 cccgcccgtc tctgccgacg aacgggttgt ccggcgggat tccgtagggc tcggggccgt
      601 cgatatcgat ccggaggatg ctcccgagca gcgtggtgat atcctgcccg tttcctcccg
      661 gtgggtggcc tctcccgacg tcgcgggcgc cgccgccgtc gccgagcggg atgtagaggc
      721 agccgtcggg gccgaagacg atcgagccgc cgttgtggtt ggactgcggc tcatcgacct
      781 ccaggaccac ccgctcggag ccgtggtccg cccggttgtt gtctgccgag accgtaaact
      841 ccgatacccg gctcgtgtgg tcccagccgc gaggtgcccc ggctctcagc ggggcgctgt
      901 agtagacaaa gaacctgctg ttctccgcga actgcgggtg aaacgcaaga ccgagcagtc
      961 cacgctcgtc gtaaccgggg cgcagcccaa caagccggcc ggtgaggtcg aggaacggcc
     1021 ggtccagcag atggtcgtcg gcgtcgatga tccggatgac accgacctgg tccgcgatga
     1081 agagccgtcc ggtcccgtcg ccgggagatg cgagcgcaac gggcgcggtg aacccaccgg
     1141 cgaccggcgt gaggcccacc tgttccgggg ttgccgttcc gggcttttct tgattgatag
     1201 tcccacctct accggggaat ggatagggtg gaagtactta aaggtacccg ggcggcgctc
     1261 accgcctcat ccaggtcaca ccgatctgga tatctcccgg gacggtgcat ccataaatga
     1321 atgacaatca ggggaagttc ttagattttg tcgtaaacat tctcccgctt cccgaccttg
     1381 atcacgtaca caatcatggt cttctttctt aagtccagaa tgacgcggta attccccacc
     1441 cgcagtttga agtaagggga tcctcgtagc gggtgcaggt gatccagagg gttggaacgg
     1501 gttctctcga cggctgcaac gatcctcgca gcagtttcgc ggggaagttg tttcaggttt
     1561 ttaagggctt tttcggtcca gatcaccgtg taaggcattc aatcgatccc cagttttagt
     1621 ttcaactcct ccgttgtgta gacctttccg gccttaatat ccgcaattgc ctcttcgata
     1681 tcacggatat cttcatccgt cagttcctcg gcagcttcct ccagggcgtc ctgcgtgaga
     1741 cggtcgatca cttcgttgta cgtctcccgg gggtagtttt tcaatttatc cagcctcccc
     1801 ttcacttccg gggtcacctt gatcgttgct tccacagcca tacaccacaa tacaccgtag
     1861 ggtaacatca atctttccag ccggaacaga acccggcacc gctatgatgc gaagtccacc
     1921 ttcggccggg tcaagaatac gcggaagatt ttgtattatc cggcgggatt aataattatt
     1981 caggggcttc cgttacctcc ggggggccgt cgatgaggcg acggcacggg ctcccgtcga
     2041 ggtctggact gaggatcatc ccggagatct cttcgccttc atctccttcg cccggccgat
     2101 gaggtattcc agttccacaa gtaccccaaa actcccgcag gcacccctgc tgcatgcctc
     2161 gttgtgcagc ctccagcact cctgttcgat gcggttgagg tcgtccatcg tataggtgtg
     2221 ataacgggcg aggatatctc catactcctg ggtgaacgta gcgatctccg ccaggaggtc
     2281 catcctgtcc ggcgaccgcg ttcttccgga agtggtgtgt tccatgacgc atgctctttc
     2341 gccacgcgga gagttatcct ttccgaaacg atgtggtaac aatgatatcc acctggagga
     2401 gtagaaaacg ggatccgctc cgaagactgc accggtcaat cccggttgat ccgtgagaag
     2461 aaccccttca ggcttcccgc aatgacgaag cccactccct gtatccatac cgtaaagaag
     2521 tccagagcga cggatgccag cgtctcttcc gggaacatat gggtgcgcac gttccggagc
     2581 gaaaa
//
LOCUS       DAJB01000240           11091 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_90912, whole genome shotgun sequence.
ACCESSION   DAJB01000240 DAJB01000000
VERSION     DAJB01000240.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_90912"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2082 a         3592 c         3411 g         2004 t
ORIGIN      
        1 aaaatgggtt agaggttctt taaggcgacg atatcggtca cgccggtaag tttccagatg
       61 tttgagcgtt cctcggaggc gatcgactcg agcggctctt cgggcttgtg accgagcgcg
      121 gcaaggatgt tctgggagag gttgtgcgcc ttgatcatgt ccacgaactt ctccctcacg
      181 atcttcttct cgatcgcgtc ctcaagctgg accaggtagc cctgcatcgt cacgaaggta
      241 tagcaggaga ggtcgcctga atacttctcg atctcgacgg agacgtatgg gtgctgccgg
      301 aaaaggtcgt tcttcctgcc gtacttggtc gcgaggaaat agagaaactt tccgtcgaag
      361 acgtacagga acggggcgat atacgggtac ttacctccct ggaatgcgat acggctgaca
      421 aatccctctt cgatgagttt gtcgtactct gctttatcca tactcgggat cttcacgatc
      481 tccatcgggc atcctcttat cccggtaacc cgcgagagta catataagtt tccacaggag
      541 ggcgcatcgt ctcccaagaa agagagaaca cccggcaact acggcccgga ataggggcaa
      601 cccctcctcc gctggactca gccccatcgg gaggggtttt ggagggtttt ctgccggcag
      661 cacctgatcc cggcctccct gaagaggaat ccaggtggcc gatccgctgg cccacggttg
      721 gcggatatcc gccgttacag gaaatcgttt caccggagaa cccatttttt caatcacgca
      781 atataataat atttatattt aagaaacgaa agaaaaatgc tgatgagcaa acaccatgag
      841 aacgccctaa ttttcttagg aattgccatc tgcgcgggat tcctcctctc cgtacctgcc
      901 gttgccggct ccggccaggg gctcatggcg ctggaatcgg gagaactgtt atcgttctcc
      961 ctcagcaatc tttcggtggc agggaacgga tcggagcaat ccgacgtccc ggcaatcccg
     1021 gacgaggaag ttacccccga tccagttccc gccccgaccg atgccggata ccctgacctg
     1081 ccccccgaca accagacaga gccaatgacg cctgcagaac cagggagcaa cacctcacct
     1141 gtcactccgg cccctgtggt cgaagcagct cccgacggga ccggcagcta cgacgacgaa
     1201 cggctggcgg ggttgatcga aaccgccagc attcgcctga tgcggctctc gatggagcat
     1261 gcccatgccc tctacctgca ggatgcagat gccgccgcta tagcggcaga cgacctgcat
     1321 gcgttctccg tcaggttgct tggcgaggtg cagccgctcc aggtctctcc ggaacagcag
     1381 cccgtcaagg acgagttcat caggatcgtg ggggcatact ccacggcaag cgagaccctt
     1441 ctcggcccga cggatgccgg cggggatgcc gttccggcgg ccttcaggga tctggccata
     1501 gcatcggaag gccttgaaac cgttagccgg caggccggcg agatgcagca ggccagcacc
     1561 cgtatctcta cgacaagcgc cgccaccgtc acgacagcag aggcacccgg cgtttcggcc
     1621 gggaccggca ccggcccggt cacggtcaca ccgcctgcgg cggcgctccc tctcctggag
     1681 cgctacacct acaacgacca gtcaggtgag aacatggtct cgctgcttgc ggagtccacc
     1741 cggaccgcaa cggtatacga ggcggtccct gccaatgcgt cgacggcgag agtcgaagcc
     1801 ggcgaagggc gcatgttcct cctcgttgcc gtcaagtcga cgaacctcgg gcacaagggc
     1861 gactccgacc tctacgccat cgagaccccc gggcgggacg cgttcgtcct tgagtgtcag
     1921 ggatcgacgt ttgagtccat ggaggtcccg tccttcacct cgctcggcga gtcgttcgac
     1981 agaaaaacac tcgagcgata tgaatcgctg aaaggctacc tatacttcga tgtccctgcc
     2041 actctcgagg tctccggggc gactctcagg gcggatctcg gggatgccgg cgctcctgcc
     2101 tggagtctcg gaaaggagct cggcaaagaa tccgacgatg caggtgcggt ataatccgca
     2161 ccgttctttt ttccacctct cagcagatca gaggtcgatc agttccacgt tccggctcac
     2221 cgtatccagc acggcgacgg tcggccggcc ggtcaggtaa ccgcaggtct ccccggggtt
     2281 gacgaccagc gtcggcccgg gagttcggac ctccgcctgg tgggtatggc cgtggaccac
     2341 gatatcaaag gctttccggc cggcgagcgc cagcaggagt tcgcggtcat ccccatgaag
     2401 cagtccgatc gtcatgccgc ccacggtgac ggtcgcaaaa gttccccgca agctgagatg
     2461 ttcatactcc gcaaaccgtg ccgaaaggag acggtggtcg ccgtcgttgt tcccgagaac
     2521 cccgatcatg ggcgattgca ggttcgcaag ccgggggatg acgaacggcg agacgtaatc
     2581 tccggcatgg agcgtcaggt ctacctgttc ccggttcagc tgcctcacgg ccgcatccac
     2641 catctggagg ttgtcgtgtg tgtcggagag gatgccgatg cgcatatatg cactcctgac
     2701 tgctgtcacg atatatatgg ctgtgcacca tcccggtggg gcgggcggtg ccgggaggca
     2761 tatcactccg ccccttcgac acgcgctgtt acgcacaaca ccctggaaaa tcctggatat
     2821 tcaggtatta tccggctatt tgaacaggaa aatctttatt taactattat ctgatacttg
     2881 agtaggaaca tactcccggg gatacaccgg gtccgggcct tgcccggtcc gggtgcatcg
     2941 ggagcgctga aaccatggac tttcagaggt acagcatgtg ggcatgtgtc aaacattctg
     3001 acggcagaac agaggaacga gaatttcctg cagggatcta tattgagatc ggggacatcc
     3061 ttgaagacgg atcggttgtc gtcgatctcc cgtaccccga cgacatcgac gacgacgcgg
     3121 aagccctgga cctctacgac gactgaaccg ggcgtgcagg ctatcggcgg aaagaacctg
     3181 cagcacgccg catcggctgg acgttcgccc cttccatctc gccgagagca aacgcgtacc
     3241 tcgcccggat ggtcggcgga aggtcgctct ccggtatcga tataaagccg tatgcggcgt
     3301 agaagtcgac gaggttccgg accgagtgca tgtacaggat gtcgtgctgg cacgcctcga
     3361 cgagtgcatc catcgccttc cgggcatacc ctcttcccct gaaccgggcc ggggtgaaga
     3421 cgccatccac ctcgtagccg tccgggtgcc ggcggcaccg ggcgacggag acgagggtat
     3481 cgttcgcgaa gaccccgaat atccggtctg tctccgggtc tgccttcaac ccgtggtagt
     3541 cgatccagat cccctcggcg agcgcgaact cctcgcttcg cagttcccgg acctctatgc
     3601 gcaggtggac cgagaccgcc agcaccagca ccggcggctt tacgcattcg aggacggcgc
     3661 tcgtaacgct cccgagcggg gcgcttcctg cggcatcacg cttcccgatg cgcgggatga
     3721 tgactgcgga ggcgcctatc tcgtctgcta cggcacagat ggtgcgcgcc gggctccccg
     3781 accgtacaag cgcctctatt tcacgccctc ccggagagag agcgcttttg agagccgcga
     3841 gccggctgcc tgcttcctgc tcagtctcct gtcccggttc gacgacgtgc aggagcaccg
     3901 ccgtcccgtc cccggcaggg tcttccagcg ttgagcggac ctggggcgtc agagccgcga
     3961 gatcggtcgg caccagcagc cgcgcaaaga gcggcggcct gccccctccc gactgcggga
     4021 cgatcaggac gtggatccgg gtatcccgga gcacggtggc cgcaacgttt cctgaaaaga
     4081 ggttccggag caggccctga tccctgaggc ccatcgcgat caggtcgaca ccccgctcag
     4141 cggcggcccg gagtatcctc tccgggacag gtgtcccgtc atcctctgct atcaccacct
     4201 ctgcggggat accctgccgc tgcaccagct cctgcatccg gcggagcgcc tcgtcggccg
     4261 gatccctgct gcccgatatc cgggcgtgga agaggactac ctcacggacg cccgggatct
     4321 cgccgacccg ctccgccatg gcggcggagg caactgaaaa gtctgtcggg atgagcacct
     4381 ttgtaaacat ggtttcacct cagattatcc ggtatccgcc cgggggcacg tgtattacga
     4441 acctcgcccc attaccgggt gtcccggtct cccggatcgt catattcgtg atggcaagga
     4501 tctcccggac gagaaagagc ccgagcccgc cgtcgatgcc gacgccgtac gtaaagatct
     4561 tctctttctc cgcctcggga acacctacac cgttgtcctc gacgcagatc gagagcccgt
     4621 cgtctaaaat ccggtaggtg acgacgacct gcgatacagt cttcccgtgg cgggcagcgt
     4681 tctcgatcag gttggaaaag acccggtcga gcatcgggtc ggcgaagacc tcaagccgct
     4741 ccacccaggg acggaccgat atcccgggga gcagcagcga cgacgtcacc tcccggatca
     4801 ccttctggag cggttgccag cccggcggcc ggagaccaag gtcctgatag tccctgctga
     4861 tctctgcgcg gcgctgcacg agccgggcat cctcttccag gctctccagg tgccggcgga
     4921 cagccgggtc gtcaaactgc cgcatccccc cggcgatccg cccgaggagt cccgatattg
     4981 ccgccgcgag atcgttccgg gtgagccgtc ccagcatgtt cagtttggcg ttcgtctggc
     5041 ggagcgcatc ctcggcgttc ttgcgggcgg tgatatcgac gatcgtgacg atgatccgcc
     5101 cgtcggggag tcggccgccc gatacgagca cctggacggc agccccgtcg gtgcggcgga
     5161 gggtcgtctc aaactcgtag acggtgttat tccggttgat ctccgacatg aacgcttccc
     5221 aggcaccggg gtcgtcgagg aatccggtga caggttctcc gatcaggttc ttcgtcgtgg
     5281 agccgaggag catggtgccc atgtagttga cctcctcgat acgcggcccg gcctcccccg
     5341 gtgcaacgag gaacgttccc gcctccgagt tgtcgaagaa cccccgatac cgctcttcct
     5401 gctcccgcaa ccggagcgag agggatgata cgacggcgcc gatggcgacg aagaccaccg
     5461 cacgcgagag tgctgagacg accagtccga cgtctccggc gaagggcagg gtcatcgcca
     5521 ggtacccgag ggcgagagcg aacgagaaga cgataccgcg gcgggggaac cagtaggcgg
     5581 caagcacgat cggcaggtac agcaggtgcg ggaggacgac ggtgatgccg gcaaaaagcc
     5641 cggcggcgtt tgcggcgaag gcgatgagcg ttgatgccgc aagcaggagg agccgggtat
     5701 cccctgaccg gcggcgcacg gagatcaggc tgctcgcccc cgaatacgga tacagaacgc
     5761 aaccggttct ccagaactcc gagcagggga taccgctcca cggcatgctc tcagcataat
     5821 ctccacaagg cgctttgact tttggccttt aaaagggatc tgatcggggg gacggcgtgc
     5881 ccggtgtcgg cggctccctt tccatgagaa caaggtcatg caaaggcata tgccatccgt
     5941 tccgccatta ctgatcagaa taatcgggaa caatcatgaa gatctcactc ctccaggtga
     6001 acacggtcgt cggcgacctc gccggcaacg ccgacaggat agcggcaggc gtcggggagg
     6061 cggctcgttg tcgacctgac ctgatcgtca ccccggagct atccctgccc ggctgccccc
     6121 cccgggacct gctgctcgat aagggattta tagggcggaa cctcaccgtc ctggaagatc
     6181 tggccgccga tctctccggt gcgccgccgg tcctcgtcgg ttttgccgcg ccgaatcccg
     6241 ccggcaccgg acggccgctc ttcaacgccg ccgccctcct ccgggacggg gcggtaggag
     6301 agacgttcca caaagcccct ctctcctcca gtgtactcga tgaaggccgc tacttcgagc
     6361 cggcggcagg aagcccccgg atcctctgcc tcggcgggag ggctgtcgga gtctctatcg
     6421 gcgaggagat ccggtgcggc ggcccgaccc tggaggtgat cgttaacctc cgtgcatcgc
     6481 cgtttgccgt cggcaggcag gtcctcctgg aggagacgct ctcggggatc gcgagggacc
     6541 accgggtcgc aatcgcctcg gcaaacctcg tcggcggaaa cgacgacctg atctttgacg
     6601 gccggagttg tgccttcagc gccgacggga cccttatcgc ccgcggttcg gcctttgccg
     6661 agggtatcgt gaccgtcgat cttgcccgcc ccgtgccgca ggcggtcgcc cccgacgatc
     6721 gggcacccga gtcggaggtc tggcgggcac tggtgctcgg cacccgcgac tacgtccaca
     6781 agtgcggttt tacatcggtc ctcctcggcc tctcgggcgg gatcgactcg tcgctcgtgg
     6841 ccgccgtcgc tgcagaggcg ctcgggccgg agaacgtcct cggggtgctc ctcccctccc
     6901 cccacacctc gatagaaagc atcgaggatg cccgggaact tggagagaac ctcggtatcc
     6961 aggtacagtg cattcctatc gctcccatga tggaggcatt cgacggggcc ctcgcggggg
     7021 tctttaccgg gcttccccgc gacaccaccg aagagaacct gcaggcccgg atccgggccg
     7081 tggtcctgat ggccctcgca aacaagttcg gctccatgct gctctccacc gggaacaagt
     7141 cggaggccgc ggtcggctat gccaccctct atggagatat ggcaggagga cttggggtga
     7201 tcgccgacgt ccagaaggcg atggtctacc ggatcgcccg gtggctgaac gcacagcgtc
     7261 ccgtcatccc cggacgggtg ctcacgaagc ccccctccgc ggagctccgg cccggccaga
     7321 ccgaccagga gatcctcccc ccctacgaca tccttgacgc gatcctccac cgccacatcg
     7381 actgcttcga gtccccgggc gagatcgtcg ccgccgggta cccggccgaa accgtccggg
     7441 aggtcctccg gatggtccgg caggcggagt tcaagcgccg gcaggctgcg cccgggctca
     7501 gggtgaccga ccgggccttc agcaccgact ggcatatgcc gatcgccgcg aaatcctggt
     7561 ggcagtgatg gcggcgcccc tcgccgaaga tctgctcgat atctacagca ccctctccgc
     7621 ggcgttcggc caccagcact ggtggccggc agaaacgccg tttgagacga tggtcggcgc
     7681 catcctcgcc cagaatgtat cctggacgaa cgcggcgcag gccgtccgca acctgcagga
     7741 cgccgggatg ctcgacccgc acctgcttgc cgcggcggat gccggtgaga tcgcccgcct
     7801 gatcgtcccg tcccggttct acaaccagaa ggccgggcgg atccgggagt ttgcccgggt
     7861 ctacgctgcc gagtttcggg cggacccggc ggcgatggcg gccgtggaga ccggcgccct
     7921 gcgcgagcgc ctgctcgccc tccggggtct cgggaaggag acggtggata ccatcctcct
     7981 gtacgcctgc gaaaagccgg tcttcgtcgt cgacgcctac acccgccgga tattctcgcg
     8041 ctacggcctc ctccccgaag gggcgtccta cgaccggaca cagcgtctct ttgcggagaa
     8101 cctcgaagag gacgtagcgc tcttcaacga ctaccatgcc cagatcgtcc gtctgggcaa
     8161 gaccgtctgc aaaaagtcgc cgctctgcga ccgctgcccc gtccgggagg ttcacggaac
     8221 gctcgcgtgc gccggggggc gggcgtgaag catataaggt agctctgggg cgcgaaggga
     8281 agtgaccaac aaccacacga acttcgcgtt ttcgcgcgag atctcgttgt ctactgacga
     8341 ttcacatgaa gtgcgactgc ccggaggata ggaacttgag aagccatagg cttcaagccg
     8401 cgacgggccg aagggctgga gcgtgagcac cgcaggtgcg agccacggag aacgcgaagg
     8461 aaagagagac gcgtatcaca gaatctgctg aagccctttt tcggtcttcc cctaccccga
     8521 tgaggttgat gctgagcccg gtttgtcccc gggatcagcc tttttcctcg agcatatccc
     8581 catgcagtgg accgtagtgg ctatcgccac gcgggggtgg ggctgacggg gagggggtct
     8641 ccccctcccc tgtctcnntc ggcctcctcc cccgccccaa gggcgggggc agtcatggcg
     8701 ataaacgacg cgtgccccga aggggcaggc ggagttcgag caccgtcagg tgcgcagttc
     8761 agtggaaagc cgtgcccctg gggccgccag cgccgggaga aaaccgatgc ccgcttactt
     8821 cttccggtag atgttcagcc cgatcttgat atcctcgtgc cccggcaccg caacgttgcc
     8881 ctcggtggag gcgatcgtga tcgacttccc gctcttggat acgccgaact cttttccgag
     8941 atccacgcgt atggtgagga cattctcctc aatcgtcata tcgatgttct tcatctgttt
     9001 agtagatgcg gttcccgcgg atatacactt gcaggtacgg cagaaagacg acaaacacta
     9061 tcccggaccc cggtcaacag tagtatcaca tcggactccg tgatgcaatg ggtgcaaagc
     9121 acactgctct tgagaaatac ttcggctata cgtcgttcct tcccgaccag gaagaggcca
     9181 tcgacgccgt gctcgcaggg aaggacgtcc tcgccgtcat ggcgaccggg agcggcaaat
     9241 ccctctgcta ccagctcccg gccctcgtct ttggggggct tacggtcgtc gtctcaccgc
     9301 tcatcgccct gatgaaggac caggtcgacg gcctccgggc aaacggcatc agggcggcga
     9361 cgctcaacag ttcgctcggc tacggggagc agaagatcat cgagcgggtg atccgcgaag
     9421 gccgcatccg ggtcctctat gtttcgccgg agcgggccgt gcagccgttc ttcctctcgc
     9481 tccttgccca gtcggacgtc aggctcatcg ccatcgacga ggcgcactgc atctctatgt
     9541 ggggccacaa cttccggccc gaataccgcc ggctccgggt gctcaaggag cggttccccg
     9601 cggtccccgt catcgccctg accgcgaccg ccatccccgc cgtccgaaac gacatcgtgg
     9661 tgcagctcgc tctctcgaac cccgcccggt tcgtcgggag tttcaaccgg aagaacctca
     9721 cctaccgggt ggtgccgaag gcccgctact tcccgcggct tgtcgcatac ttaaacgagc
     9781 accgggacga cgccggcatc atctactgct tctcccagaa ggcgacggaa gacctcgcgg
     9841 agaagctccg gggcaagggt ttttcggccc tcccctacca tgccggcctc cccgatgccg
     9901 tccgcgcaga gaaccaggag gccttctccc acggcgacgc cacgatcatc tgcgccaccg
     9961 tcgccttcgg catgggcatc gacaaacccg atgtgcggtt cgtcatccac accgacctcc
    10021 cgaaggatat cgagtcctac taccaggaga ccggccgggc cggaagggac ggggaggcga
    10081 gcgattgcat cctcttctac agtcggggcg actacaacac gatcaagtac atcatcgaga
    10141 aggagtgcac tgatgcggcc aaagacgcag cctaccggaa agcgggggcg atgctcgact
    10201 actgcgagac caccgggtgc cggaggaagt ttctgctcgc ctacttcggc gaggcctatc
    10261 ctgaagaaca gtgcgggggg tgcgaccggt gcgagacgcc ggtgaaggtc tttgatggga
    10321 cttctgtcgc atcgacgatc atcgcctgca tccacgaggt cggggagcgg ttcggagcgg
    10381 cctacatcgc agacgtgctc tccgggtcga agagcgcgag aatccgtgag aacgggcacg
    10441 atgccctcca cgcctacggc tcagggggat acacccgcga ccagtggctg ctcttcattc
    10501 aggaactggt catgaagggg ttcatcacgt ctaccggcgg ccggtacccg gtcctcatgc
    10561 gaaacgaccg gagccgggcg gtggtcgcgg gcgacctccc ggtgccgctc acagagccac
    10621 gcccggaagg cgtcatcgcc tcggaagtcg aggatgacta cgacgagacc ctcttcctcc
    10681 gcctccgcca gctccggaag gtcgttgccg acctcgagca cgtgccgccg ttcgtcgtct
    10741 tccacgaccg gagcctcaag gagatggcga agtgctaccc gggcaccggc gttgcgctcc
    10801 tccaggtcta cggcgtcagc gaggggaaac tacagcgcta cggcaggcgg ttcctcgacg
    10861 ctatcgataa acactgcgtc gcgcagggga tcggaccgga gcgccggagc gggtgacccc
    10921 ggcgggagga accgggtcac ggatctgtag caccgtcgcc gcaactacag ttttctgaca
    10981 aatcactata taaacctgta atcgagagac tgtattggtg gaaactgatg gttgcactta
    11041 ctgaagagat gaaggcagcg tttagcacga tgaaggcctt cccggtcgct a
//
LOCUS       DAJB01000241            8521 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_69378, whole genome shotgun sequence.
ACCESSION   DAJB01000241 DAJB01000000
VERSION     DAJB01000241.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_69378"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    8281..8301
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1520 a         2636 c         2726 g         1614 t
ORIGIN      
        1 aaacgttccg ttcgggtcgg aaaactcggc gagggccttt tctttgccgt ttgcgtgtgc
       61 ataagccacc gcgctctcga cgaaggcgac gagcgtttcg ttcgaggtgt agttttcctg
      121 ctccggggcg tcagggcttc ccggcgttga agcggccgtg tcgggctggc tctgcgtgca
      181 cccggcggcc ataaggagca gacccaggac acacacggat acgaatgaca ttaacgatgc
      241 attcgtgcca ttcaatctgt attccctccc ttaaacgagt tagaacggtt atacggacaa
      301 aggctctttt gaatccctca atcttcaccg cggtcatcgc tgcttcgctc atccggggga
      361 gtatcctctt cctgagcatg ggggttaacg cgtgaccggt gccccggacg cgactaaaga
      421 atgaggtata aattccccaa gggcggtaca tacattactg ggagtggatc ggggatcgtg
      481 cctgccgggg gtgctgtcat ggccagccgg gttctccccg ggtctgctga tccgccctgt
      541 acggcagcgt ggtagagtga gtgtccggag agcatcatca cggggggatg atcgtgaaac
      601 acagcggcaa ccgacctcaa gccgggatac ctgagtttgc agccgatatg ggcgggtgtc
      661 aggctttgca tacgagtgag accatgagcg aatcgttgag agtgatcttg atcggggaga
      721 atcccggcga cgccccgggt atccgggaga tgcttggagg gtgcgggcgc gaggttgaac
      781 tgctgtactg cgagcgcccc gaagacgggc ttactccggt gctggagggc ggaatcgggg
      841 tggttctcct cgacctcgac ttcccggacg gccggggaat gggaatcttt gaccgcctgc
      901 gagaggtggc gcccgacatc ccgatcatcg tgctcaccgc agcgggagaa gagcccaccg
      961 cactccgtgc aatgcagcac ggggcgcagg actacctggt caaagggaag accgacgcgg
     1021 aactgctctg ccgggctgtg cggtacgcgg aggagcggac gcgggtggaa cttgcactcc
     1081 ggcactcgga ggcgatgtac cggagattgg tcgagaacct caacgaaggc gttctcgccg
     1141 tggacgaggc cggcctcatc accttcgcaa acccggcgat ggaggagatc ctcggctgcc
     1201 cggcggaaag cctgatcggg tcgccggtta ccgggttctt cgggaccccc ggcagcaccg
     1261 gtacggccac ccgaaatccg ttctgctggc agaccgctca gagacaggag tttgaactgg
     1321 acctcctcag cgatgacggg agaagcgtcc acgcgctcgt ggtcacgtcg ccgatcacgg
     1381 atgccaccgg cgccttccgc ggatgtcttg ccggggtgct cgacatcacc ggccgcaaaa
     1441 gggcggagga ggcgctcagg cagagcgagc agaagtaccg gcttgtcgtg gagagcctca
     1501 gcgaggggat ctgggtgatc gaccggaacg cctgcacatc gtttgtcaac ccccggatgg
     1561 cggagatgct cggctacgct ccggacgaga tgatcggcag gtccatctat tcgttcctca
     1621 cccccctctc cgatgccacg atgcgggatc gcctgaactg ccggagagac ggcactcagg
     1681 aacagtatga gtgcgagttc atccggaaag gaggcgaccg catcaccgcg ctgatgatcg
     1741 cgtcgccgtt ctccgatgat tccggcgaat tccggggctc gatcgccggt gtcatggata
     1801 tcaccgagcg aaaacgggcg gaagaggaga tccgcatccg gaacgagcag ctcatggtgc
     1861 tcaatcaggt ggtcggcgtc tccgccacct ccctctctct tgccgaactc ctggaagagt
     1921 cactggaaaa gacgctcaac ctgatggggt ttcatgtcgg catcgtctac atgctcgacg
     1981 ccgagcgcaa acgggccctc ctccagcacc agagcgggct gccccaatca tgcatggccc
     2041 ggagtcgtat catcaaggtc caccactggc cgttcaactt tatcttcatc gccggccagc
     2101 cgcgctacat agagcggcag cctgacctca actccatcga ggcgggcata ctgcaggagc
     2161 tcgaggtctc ggcgctcgcc tgcatcccgc tcattgccga atcggtcgtt gtcggggcgg
     2221 tttacgtcgg cagcaggaca aaggaaacct tctcccgcga ggagatggcg cttctcgaga
     2281 tggtgggaaa ggagatcgga tcgggtatcc tgaagggcat gctgcacaag aaactggagg
     2341 cggcgaaccg ggaggcaaac ctctacctgg atatcatgac gcacgacatc aagaatgccg
     2401 agaacgtctc gaatctctat gccgatctcc tcatcgagat gctggaggga gaggccgcgc
     2461 tatacgccag gaagatccgg agcagtgtcc gcaggagcgc agagatcctc gggaacgtca
     2521 ccacgatccg ccgcatccac cacgattcac cggatgtcgg gccggtcgac ctcgatctcg
     2581 tcatcaggga ggaggtcgcc gcctttccgg atgtcgtgat tgagttttcg gtcagccacc
     2641 gccgggtctg ggcggacgac ctcctctccg aggtcttcgc caacctcatc cgcaatgccg
     2701 tcaagttcgg cgatccgggg atccggatcg ccatccgtgt ggaggagtac gacggtgaga
     2761 gcgtgctggt ctctgttgag gataccgggc cgggcatacc cgatcccatg aaggagtcga
     2821 tctttcaccg gttcgagcgg ggatgggtcg ggggctacgg cgacgggctc ggcctcttca
     2881 tcgtccgcac cctggtcgag cgctacggcg gcaccatccg cgtggaagac cgggtagagg
     2941 ggcgttcaga cctcggtacg gcgttccggt ttacgctccg cgaggttctc gcttccgggg
     3001 acgatgaaga cgacgagtat ccggacgggg aagagtgcga gtgacctccc gggcatcccc
     3061 ccggagagtc tcccgcgcgg gaacggcgtg atgtcggttt cacatcgggt tgggggctga
     3121 atttttgact caatatcgaa aggttttaag tggtgaggcc acaatttagc gtcgtggcat
     3181 ctctctatat ccgggtcact cagttctttg cgtcaatagc ggtctgcctg ctcgcagcgg
     3241 tgatcgggtc ggccttcaca acgccggcca tcccaacctg gtatgccgcg cttttgaaac
     3301 ctgccctgaa cccccctgca tgggtctttg gtccagtctg gacggtgctc tatatcctca
     3361 acccgtaaga ccgctcactc tttttctatc tgccgccaga cgtgggcgaa acgctgaaat
     3421 gcggtgtagt gcccgaggat gccgaatata acgaggagcc acccgaggta gttcagacca
     3481 tagacctctc ccgggatcag caccgtgagc acgccggcga tgatgatcag cacgagacgg
     3541 tctgcccggc cgaggacgcc cccgtaaaac ctgccgaccc cgacagcctg cgcctgggtg
     3601 ccgaggtatg aggacatcag gaccccggtc agggcgaaga cgccgatcgg ccagggtgcg
     3661 gcgccgcctg caaatatgcc cgtgatgatg aatatatcgg cgtaccggtc gatgacatgg
     3721 tccaggaaat ccccgcggac gcccgcgatc cccatgtccc gggcgagcgc tccgtcgagc
     3781 gcgtcaaaga cggcgttgaa gatgaccatg gcgacgccga gcacgacccc tccggcatag
     3841 aacgcgatgc cggcaagcgc cgatacaaaa aaggaagcaa tcgtcagccc gttcggggtg
     3901 accccgatct ttctcatcag gcccaccatg ggctgaatga tgccctgcac ctgcggtcga
     3961 aactggtcga gcgtcatatg ccgggctcca gataatcggt ccagtcgatg gacccgcagg
     4021 acggcggcaa ctccccccgg atgatctgct cgatccggtc tgcacactcc tcgacggaga
     4081 gatcggtcgt atccacctca aagacgtgct cgtctgagtg ctcatcgagc gtctcgatca
     4141 ggatgacgtc gagcgcctct gcctcggcgt tctccgcgac cttctcgggg ggatagttcc
     4201 tggggccaag acgcttttgc aggacgtcgg gccggcaccg gagcaccacg acccggtcgc
     4261 aggggagcag gtgtgcgagg tgcccttcga cgattccgtc gatgggttcg aactcctccg
     4321 cccataggtc gacgtccacc accagcgtct ggcgggcgca gtcctcctcg atgatgtagg
     4381 gccgcaccgt atcggccaga cggacgaccg tgtgccctcg ccgctcaagt tcggccgcaa
     4441 tggatgtctt tcctgttccc ggcgtgccgg tgatgcctgt catcataagg ttttaatcgc
     4501 ctcgaggaat cgaatgtttt cccagtcttc gccgatgctg acccggatat agtggtcccc
     4561 gagccccggg aaactggtgc aggaccggac gaggacgccc ttcgccgcga ggcgctccac
     4621 cgcctcgtcg cctgttccgg gagcgacgtc gatcagaacg aagtttgcat cggtagggta
     4681 tgtcttgaac ggcacctctt caaggaaccg ctgcctccac tgccggacgt ggtcgcgggc
     4741 ttcaagcgcc cggtcggtgt cggcaagcgc cccgacggcc gccgccaggg agaccgagtt
     4801 taacgcaaac ggtgtcgccg cccggtggta gtacggctcc agccacccgg ggacgaaggc
     4861 gtagccgacc cgcagcccgg cgagggcgaa tatcttcgac atcgtccgcc cgaggaccag
     4921 gttctcgtgc cggcgcatga gaggccggta gtcgatatcc gcaaagtcga cgtaggcgtt
     4981 gtcgaggaag agcagcccct ccatcccctc gaggatctcc tcgaccgcct ccggcggtac
     5041 cgagttcccc gtggggttgt tcggagagca gaggaaagcg agttttgccc cttgagaggc
     5101 ctcgatgaac cctgccgggt cgaccgagaa atcctcccgg cgggggacgt ttgtgacccg
     5161 ggcgccgtat atggcggccg cgagaccgta gaaggagaag gtcggggtcg agatgaccac
     5221 cgtttccccc ggctcgacga cggtccggat caccgtctcg atgacaccgt ccatccctga
     5281 gccggtgacg aaccggtagt cgccgtggta gcggcggaga gcctcgacga gcgccgatgc
     5341 ccgttcgtcc gggtaccggt ttgctgtctg gagagcgttc gccgccgctt gtattgtggc
     5401 gggcgacggc ggccgggggt tctcgttgct cgcgaggcgg gccacccggt cgatgccgtg
     5461 ctcgcgcgcg acgtcctccg ctttcttcgc gtaggagtag ccgcttgcgc ccgcgaagca
     5521 cgccctaatc aaacgccgca ttaatgacac cgacagctgc gtcgatctcc tcgtacgtga
     5581 tcaccagcgg cggaatcagg cggaggttcc cgtcggcggc acagttgacg agcacaccgt
     5641 gctcggcaca ggtgcgctgg atctccgcgc accggtcacc gaccgagatg ccgatcatca
     5701 ggccgcgtcc ccgcggattg tggggagcaa gttctctcat gaaccgcttg cccttcccgg
     5761 ggatatcggg gaggacctcc tcgatgacgc cgatcgtcgc gagtgccgct gcgcaggcga
     5821 gcggccctcc ggcaaaggtg ctgccgtgct cgctccttcc aaactcaagc ccctcgcggg
     5881 cgacgagcgc gccgatcgga aagccgctcg cnnccggtct gcacctcgtc gacgatcatc
     5941 agtgcgccgg tgtcgtcgca gatatcccgg ataccccgga ggtaactgtc gggcgggatc
     6001 aggacccccg cttcgccctg gataggctcg acgaagactc cggcggtatc cttatcgacg
     6061 gcaccctgga gggcgtcgag gtcgccgtag tccacgaagg tgcaggcggg cgagagcggc
     6121 tcgaacggct cccggatctc gggcttatgg gtgacggcga gcgacccgca ggtcctgccg
     6181 tggaacccgt gggtaaacgc gacgaacttc tttcttcccg tccggacgcg ggcgagtttt
     6241 attgcgccgt cgtttgcctc cgcgccggag ttcgagagga acgccttgcc aagccccgtg
     6301 atccccacga gtttctccgc aagttccgcc tggttcggga cgtagtagag gttcgaacag
     6361 tggatcaggc ggtgggcctg gtcgcagata gcctctacca ccttcgggtg gcagtggccg
     6421 gtgctgcaga ccgcaatccc ggccacacag tcgatatact cccgtccctg atcgtcccag
     6481 acgcgcgacc ccgcaccgcg gacgatcttc atcgtccggc cgaatgcggg catgtagtag
     6541 cgtgcatcaa gcacccgtga atcttctgcc atggtctcac tcgtactcga tcgtcgcggg
     6601 gggtttgggg gtgacgtcgt agacgacccg ggcgacgccg ggtatctcgg cggtgatccg
     6661 ggctgccatc cgcgcgaggg tgtcgtaggg caggggcagc gggtcggcgg tcatgccgtc
     6721 ccgtgactgg acggcccgga ccgcgacgat ccagccgtgc agccggatat ctcctttgac
     6781 cccggtcccg aggccgagga gcgcggcaaa gcactgccag ggttggtact tctccaccag
     6841 gcactcctcg acgatggcgt ttgcctcccg gacgatcgcg atcttctctc tggtcacctc
     6901 accgagcacc cggacggcaa gcccgggtcc cgggaagggc atcctgtgct ggatctcagt
     6961 cgggagcgaa agcccgccgg ccacatcgcg gacctcgtcc ttgtagaggt cggcaagcgg
     7021 ctcgatgacg cctttgaact tgatatcgag gggcatgcca cccacgttgt ggtggctctt
     7081 gatgccccct tcgctctcga tgcggtcggg gtagatcgtc ccctgcagga gcatcgtcgc
     7141 tcccgtccgc tttgcctccc gctcgaagat ccggatgaac ttcgcaccga tggctttgcg
     7201 cttctcttcg ggatcggtga tcccggcgag cgcctcgaag aactcatccg ccgcatcgac
     7261 gacgcggagg tttgcatcgg caaagaggga ctgtatccgt tcggtctctc ctttccgcat
     7321 gaggccggta tccacgtata tcgggacgag ctgatcaccg atggcacggg tggccagcgc
     7381 cgcgcagacc gacgagtcca cgccgccgga gagtgccatc acgaccttat ctccaccggc
     7441 agtttcgcgt atctgccgga ctgcctgatc gataaacttc tcaatagtta ccattctgtt
     7501 gtctcacctg gtctgggatg atattttatt ctttttgcat gcctctacaa agccgatgta
     7561 gggcggggag gggtttgtgg ggctcgattt gaattcgggg tggaactgtg tggcgaggta
     7621 gaaggggtga tccgggagtt cgcagacctc catcctctct ccgcaggagc ccgagaagac
     7681 gaggcctgcg ttctcaaggt cggcgatgaa cttcgggttc acctcgtagc ggtgccggtg
     7741 ccgctccacg attgcggtcc tgccgtagag gccgnngaga cctcctcctg ctcggggagg
     7801 atggcgatga cgtgcgcccc gggtcccatc tcttcgctgg tggcatcggg aatgccgagg
     7861 acgtgccggg cgtactcgat caccgagagc tggaagccga ggcagaggcc gaggtagggt
     7921 ttgttgttct cgcgggcata ctgtatcgcc cggatcttgc cttcgatgcc gcgcttgccg
     7981 aaccctcccg ggatcaggat gccgtcgaca tcggcgaggt cacggagttc gaaggtctcc
     8041 gcatccagcc agcgtatgtt cacttcggtg gagagcgccc ggcccgcatg cttgagcgac
     8101 tctttgatgg agatgtagac atcctcgatc ccgtacttgc tgacgatggc gaccgtcgcc
     8161 cggttcgtgt actcctgcga aacgacccgg taccatgcgg tgtccgggtc cttgttatcg
     8221 agggccaggt aactgcagac gacgtccgca agcccttctt tctccagctc caccgggatc
     8281 nnnnnnnnnn nnnnnnnnnn ncccacgatg atgtcgggct gcagcccgag ttcccgcaga
     8341 gccttgaccg agtgctgcgt gggtttggtc ttgtggtcgc ccatggtgtc caccgggacc
     8401 agggtgacgt ggaccaggat catatcctcg ggcgcaagtt cgccgtgcat ctggcgaacc
     8461 gcctcgagga acggcatgct ctcgatgtcg ccgaccgtgc cgccgacctc caccaggcag
     8521 a
//
LOCUS       DAJB01000242            4594 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_132710, whole genome shotgun sequence.
ACCESSION   DAJB01000242 DAJB01000000
VERSION     DAJB01000242.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_132710"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2640..2673
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3449..3472
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          838 a         1387 c         1484 g          827 t
ORIGIN      
        1 ggtgcagatc gggatatggt cccacacaga aggcaggccg tgagggatgc gaagggcggg
       61 aaaggaaatt catagagtta aatgacaacg agtactagtt attccatctc ccggcagtac
      121 ggccgatctc cagtggaaat atgccttgat ccccggatcg cgggtatgag tgcatactcc
      181 ggtaagaacc ctgcatacct tccccgaata tggaaactcg gcggaataat cggcctgcct
      241 ccgatgcagc ccctcccggg gagcgaaagt gcgcgcccgt ctatcatcgg atatccgtcc
      301 gcatgaaccg cgtgtatgcg acggcaaaga agacgacggg atagaggatc agcgccacca
      361 cgctcgacca tatctcgccg agcgtatctt caagggtcgc ctcggggagg cgtgaatcct
      421 caggatcagg atgctcctca gggaggtcct tcgagcatcg tcaggtgctc agcccggtga
      481 accctcgtgc ccgggaattg cgatcgccgg gagcaggcgg ggaaggggtt caacaaatcc
      541 ggtttttctc gtatcggctg gttcccgggg cactacccgg catgacgggg agccgggatc
      601 ggtcagacgg cgaaaaatcg ggacggtgac gctgtcggga aaattaatgc tacaaataat
      661 ttatatcgta tcacttaatc ttaaaaatag taaattatat aacactcctc gccatatatc
      721 tgcctggcgg gcggtggcgg gaccgccggt gccccggccc gggccacaga tgggccatgg
      781 ccacctcacc gggcctcccg gagttcattg ccgctgcctg ccgaactgcc ctgccggtgg
      841 cggggcggcg gtgactgagc atgaaggaat gtattcaatc cgagaaaggc gagatccttt
      901 tcgacaccga ccgtgacgcc tggctctacc tcgcgccgcg gggcggtgac gcaggcgggg
      961 cattcgggcg tggaagggat ctctacctgc acgaacagga ggggctgccg gatatctact
     1021 acatccactc ctggtcgatg gagcccggcg aagacgagca gatccagact atctcaatct
     1081 tatccgccga ggagttcctg gagtcgcggg ggctcgtgct tgccgcatac ccggagcagc
     1141 gggggagcca gatactgcga tcgtacggct acggcatcgc ggaagagttc tgatatctca
     1201 cggtcccggg cgggggcggt gtcgccctcg cccgccatga gttataccga atagaaatta
     1261 tttaacatca attgcatcct attttctcat atgtcatact ccgcacggag gaacgtcctt
     1321 ccgttcaccg ccatcgtcgg ccaggagacg atgaagcgcg cactccttct caacgccgtc
     1381 aacccgggaa tcggcggcgt actcatccgc ggcgagaaag ggaccgcaaa atccactgcg
     1441 gtcagggcgc ttgccgacgt gctccccgag atcgatgtgg tccggggctg cccttacagt
     1501 tgcgatcccg caggcttcgg cggtttgtgc agcgcctgtg ccgaaaaaac aacatccggc
     1561 gaggatctgg acgtagaacg gcgccgggtc cgcgtcgtcg acctcccgct tggagtgact
     1621 gaagaccggg tggtgggcac cgtcgacgtg gagcgggcga tccgtgaagg aacggttacc
     1681 atcgatccgg gtatcctcgc ggcggtcaac cgtggtatcc tctatatcga cgaggtcaac
     1741 ctgctcggcg atcacgtggc cgacgtcctc ctcgacgctg ccgcgatggg cgtcaacgtg
     1801 gtggagcggg agggcatatc attctcccat cctgcccggt tcatcctggt cggcaccatg
     1861 aacccggagg agggtgaact gcggccgcaa ctgctggacc ggtttgggct gcaggtgacc
     1921 gtggagggga tcgaggatcc cgccaagcgt gtggcggtca tcagggccgc cgaggcgttc
     1981 gagcgcgatc ccggagagtg ccgacagaca cacggcgcgg cgcaggagga actgcgcgaa
     2041 aagatcgccg atgcgcgggc gctgctgccg tccgtcacgg ctgatgatgc tctcctggac
     2101 gcggtggtga atgcatgcat caggcttggg gtcaggaccc accgcgccga tattgccgtc
     2161 gtccgcacgg caaagacgat tgccgctctc gacaggcgga ccgtcgtcac ccgcgatgac
     2221 gtccgggaag ccatggcact cgcgctcccg caccgaatgc gccggaaacc gtttgaggag
     2281 ccgaagatcg acccggagca gctcgacgaa gcactgaagg atacgggaag caggcacgac
     2341 cacgatcact ctcctcatga tggcgggggc ggcccggccg gtgatgagtg tcccggcagg
     2401 agcgacggag aactgtcccc ctccggcggg agtgcagcac atgagactgt cctcccggtc
     2461 ggagacccgg tcgacgtcgg aaagatgagc ctgccggagc ggcgggacaa ccaggagcgg
     2521 cagcgggcct ccggcaggcg gcttgagacg ttctcggcgg agaatgtggg aagttatgtt
     2581 tctgcgcggt atcccaccgg caccggcgat gtcgcgctcg atgccacgct ccgggccgcn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggttcga tgggaaccat gatgcggatg
     2701 gaatcggcca agggtgcggt tctctcgatg ctggtggact cctaccggca ccgcgaccgg
     2761 atcggccttg tggcgttccg gggaaacagc gccgagatcc tcctcccgct ctgctcaagc
     2821 gtcgatctcg cgcagaaaca gctggaggat ctcccaaccg gcggaaagac gccgctttgt
     2881 gccgggctga tgaaaggcat ggaggtcctc ctgcaggagc gacggaagaa cggcgaggtg
     2941 atcccgatga tggtcgtcat ctccgacggt agggcgaacg ttcctctcgc gggagatgtg
     3001 cgtcaggaga ttgtcggtat cgcagaggag atccggtcgt acggcatcca tacggtggtc
     3061 atcgataccg aggagatcgg ggactctttc ctggagttcc ggctgggtta ctgcagggcc
     3121 gtcgcggagc atgccggcgg cgggtactac ccgatcgccg acctctctgc cgggctcctg
     3181 cacggcatca cagcggccga gcgggatgcg atggtgcacc ccgcctgaaa actgttcacc
     3241 ggcaaagcgg caggagtttc tcgatcaggc ggtccacctg ctggactgct gtcccgatgt
     3301 aggcgtccgg gtcgaggagc ccctccaggt cttcgcggga gacgaacttc gtgacctcgg
     3361 ctcgctcacc gagcacctcg gcgaggtctc ggtcttcggc gagagcctgc atgctcgccg
     3421 tccgcatcac ttcgtgggct tcctgccgnn nnnnnnnnnn nnnnnnnnnn nnccttccat
     3481 cacgccgatc atcaccttga ggatgtggtc ggtcagcacc gacgcctcgg ggaagagcac
     3541 ccgctcggtg gaggagttcg tcaggtcgcg ctcgtcccag aggacgttgt ttgccagggc
     3601 cggttcgacc gctgaccgca cgatccgggc aagcccgcag acctgctcgc tcttgatcgg
     3661 gttcctcttg tggggcatgg tgctcgagcc gacctgattc ttcccgaacg cctccgcgag
     3721 ttccccgatc tccgagcgct gcatcagccg gacctccagc ccgatcttgt cgagcgttgt
     3781 cgcgatattt gcgaggagca tgaagtactc cgcgtagcgg tcccgcgata cgatctggtt
     3841 tgagacatcc acggactgga tcccgaggaa ctccatcatc gtctcctgga tctcgatgcc
     3901 ggcctcgccg agcgctgcct gggtgcctac ggcaccggtg agctgcccga cgacgacccg
     3961 ggagcgcatc tggcggagcc gctcgatgtg gcgggagacc tcactcgccc agatcgcgaa
     4021 ccggaggccg taggtggtcg ggacgccgat ctgcccgtga gtgcggccgg cgcagacgag
     4081 ggtctttgtc tcggcgctcc gggtgaggag cacgcagagg agtttgccca gtttctcctc
     4141 gatgagcgcg aggctgtcgc ggagctgcag ggccgtcgcg gtatccagga tgtcgtttga
     4201 ggtcgcgccg tagtggaccc accgacccgc gtcgccgcag acctcggtga cggccttgac
     4261 gaccgccatc atgtcgtggt tgatctcggc ctcgatctcc ttcgcccgct caagacttgc
     4321 ctccaacgcg caggccgcga tcgtctccgc atcttcgcgg ggaatcatcc cgtgcacggc
     4381 ttcggcacgg gccagcgcca cttcggccat gacgatcgcc cggaaccgat tctcctcatt
     4441 ccagacggca cgcatctcgg gggtgccgta ccggtagtcg atgggatgga ttgccatagt
     4501 ggagaagtgt tggcattctc gctacaaaaa agggggtgcc gcccctcttc ccgcgccgca
     4561 gggctcgccg tcatatacga cgggcaagga gacc
//
LOCUS       DAJB01000243            7703 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_37265, whole genome shotgun sequence.
ACCESSION   DAJB01000243 DAJB01000000
VERSION     DAJB01000243.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_37265"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1483 a         2447 c         2410 g         1361 t
ORIGIN      
        1 gtgagacagg agcaggagga gcagggcgtg gtgatcacct ctatcccctc gaggagctcc
       61 tcgatcggca ccccgttaaa gaggaggctg ttcgattccg cgggggcatc atcggggagg
      121 acggtctcgt ccacccggac gccgatcccc tccatctcaa ggagcatccg gatctccgcg
      181 agaacggccg aaagcgtcat cccggtctcc tcaaactctt cgggggtctt ctcgatctcg
      241 tttccgatgt gtttccattc gatgacgagt tccttcttca tggtgtacct cctccattgg
      301 tggtggcagg acatgtcact gtcgcctccc cggcatacca ccggtcccgc agtcggaggg
      361 caacgttcac aagcgatatc agcaccggca cttcgatcag cgggccgatg accgtggcga
      421 acgccacgcc ggagccgatc ccgaagaccc cgatagcgac cgcgatggcg agttcgaagt
      481 tgttgcttgc cgcagtaaac gaaagcgcgg tagaccggcc gtagtcgacg ccgaccttcc
      541 aggacatcca gaacgagacg aagaacatgg cgaggaagta gatcgtgagc ggaatggcga
      601 tccggacgac gtctaggggg agggcgacga tgtttgcccc ctgggtcgag aacatcacga
      661 cgatcgtaaa gagcagggcg acgagggtga tcggcgatat cctcgggatg aaccgctcct
      721 ggtaccacgc cttcgatcgt gcccggataa gcgtaaaccg tgtcagaaac ccggcgatga
      781 acgggatgcc gaggtagacg aagaccgtca cggcgacgcc cgcgatcgtg atcggcaccg
      841 ccgtcccggc cccgatcccg aggagcggcg ggaggacggt gatgaagacg taggcgtaga
      901 gggcatagaa gaggacctgg aagacggagt ttaagcccac gatcgcggcg cagtactcgc
      961 agtcgccgca ggcaaggtcg ttccagacga tcaccatcgc gatgcaccgg gcgaggccca
     1021 cgaggatcag gccgtacatg aactccggcc ggtcgaagag gaagactgct gcaaggatga
     1081 acatcaggac cgggccgacg acccagttct ggacgacgga gaggccgagc acctttcggt
     1141 cccgaaagac ccgcccgagt tcttcgtagc ggaccttcgc aagcggcggg tacagcatca
     1201 ggatcagccc gatcgcgatg gggatcgatg tcgtcccgac cgcgaagtcg gtgatcgctg
     1261 caggcaccgc ggggaagaga tagccgacgg cgacgccgac gaccatggcg aggacgatcc
     1321 aggctgtcag gtagcggtcg aggaacgaga gccgcgagcc ggcggcctgt gagtggatct
     1381 ggtcgacggt gcccatcgcc ggtcaccccg agcatccgca gccgccggag cgccggtcgg
     1441 cccgaaactc cctcgccagc gcggcggcat acaccgcccc ggcaccgggt aggatatact
     1501 tatgctcgca cgcccgccgg acctggcgca gggcgtccgc ggcacggcag ggtatgttct
     1561 ctcgtcttgg attcattttt tcacctcata cgtggtcaga tcacgctgag cacctggaac
     1621 aggacgccgg caaggacggc gaccgtgagg atcgtcgcca cgaaggcggc gaccagcctc
     1681 cgctcgaaga tcgcagatag aagcgtcacc tccgggatgc tcgcccccgc gccgccgatg
     1741 atcagggcca tcacggcgcc gattcccatc cccttctcga ggaggacggc gctcaccggg
     1801 atgagcgtct ccgcccggat gtacatcggc accccgatca ccgccgcgac cgggatggcg
     1861 agcgggttat ccggccccgc gagggagacg atcaggtctt cgggaatgaa cccgtagatg
     1921 aacgccccga tccccgcgcc gaggagaaga taggggaaga cctgcttgaa aaggctgacc
     1981 gcgaaggaga acgctccccg gatacggact gcgtggcccg cggcgcagtc gcagggctcc
     2041 ggccgccctt cgaccatcac gtctttgacg tagcgctcgt agccgagccg gccgaggagc
     2101 cagcctatga cgacggcggc gggaaacgtg acggcggcat atatcgcggt cgggacgacc
     2161 ccgacgagcg cgacgaggag ggcgaggatc accgggttca agagcggcga cgcaaacaga
     2221 aacgacatgc agaccccgaa cggtatcccg atatcgagga ggccgagcag gatggggata
     2281 gtcgagcagg agcagaacgg ggtgagcgcc ccgaacccgg ctgcgaggac gttcccgaca
     2341 ccctcccgcc tcccggcaag cacgcggcgt atccgctctt cagggatgta ggcctggagc
     2401 agcccgacga ggaacgtgat cccgacaaag aggaggacca gttcggcggc tatcacgacg
     2461 aagaactgcc cggcggtgat gagtgttgcc agaagatcca ttctgtcgct cccgcaccgg
     2521 gggtttactg ctctcctgcg tcagactctt ccttcttctc cttcgggacg atcctcgtcc
     2581 cgcagcagct gccgccgcag ccggaggaga agaggttctt tatccgcgat ccgatacatc
     2641 cttcgtcttt tctgtcattc tcacctttca tggtcatcac atccgctccg gcaggtgcgt
     2701 cggagtgttt caacccggtt tgaactactg ttggtcgtat aggtgtatga agatttctat
     2761 ttcaataaaa gttgaatcta tacggcatgg cgcgcaggag gaacctgtcg gacgatgtag
     2821 tcatcagacc ggtcgtggat agcagtttga tgaatgtccg gcgcttttgc aggagcagga
     2881 acataccgag cagccgttca ggctctcaaa cgtggcaagg atggctcttg aatgtcgctt
     2941 gcggaggatg catggcagat gggcgacgcc gggagcgact cgcacccggc agcatccgac
     3001 ctcacccaaa cagcattgcc atgagaagat atgccccgat cccgatgaag agtaccccca
     3061 caatgcgccg catccagtat tcgagcctct ccgccgcctt catcgcgctc cccaccttag
     3121 caaccccggt caccatgatc agcgagaacg cgatcaccgg cagagcggtc gcgacggcaa
     3181 agaccgccgg gacgccgagc ggatcagagg tccggagcgc cagcgggacg agcatcgcaa
     3241 agaagagcac cgccgaaaac gggcagaagg tcagcgcgaa gagtacgcca aggatgaaac
     3301 ttcccgcgag cccccggtcg gcgacccgct ccttgaacgc cgcaaacttc ctgctgccgc
     3361 ctgtccagga ggggagcggg acgagatcga gcatcacgac cccgatcgcg atcagcagcg
     3421 ggccgacgag cacctcgccc caggtctgga ggaagagcga gatcccctga acggagagcc
     3481 cgagggtcac aatcgctgcg gcgatgccga cgtagacgac catcctcccc gcgctgtaga
     3541 gcgccccggc gaggagggtc tgccccgggc tgccgatccg gcgggagagg tagccgatcg
     3601 cggcgatgtt cgtcgcgagc gggcaggggg agagggccat catcagcccg atgaagaacg
     3661 cggcgacgag gggaacactg ctcgtcccca tcgtctccat gaaccccatc aggctcattg
     3721 cgagaagtcc ccggcgagac gcatcccgat cagcgtcttg aggtactgca taaatccagg
     3781 cttgtcgttg agtttgtacc agacccgaat atcctcttct ttagagaacc cgccctggtc
     3841 gtcgtaggta ccgatccaga gcgacgagcc ggtcgcgccg taacgctcga cgatctcctt
     3901 cttttccggg tcggcgacgt cgacgtgatc gaagatcacc ttcccggagt cgagttcacc
     3961 ggggaagtgc gtcttcacgg tctcttcggc gtagtcgccg agcaccacgc aggagtagca
     4021 ctggttcggc gcggtgaagt ggatgacctc gactttcgtg acggtgccgt tgagcggcga
     4081 gatggtgact gcgtccgctc cgccgggttc cgtcgtgcac cctgcaaaga gggcggcaca
     4141 ggcgaggagc accgccagaa cggctaacgg caggatctgc cgggcagggg cgctgcaggg
     4201 gaactgcccc tgcgtgcgcc cgggtccgga gttactcacg tgtgttcgtc tcatcaggga
     4261 cacccctggt gctggataga tcattacaac agatcttgaa atgacgcaga cctgttgttc
     4321 agccgggtat atagttcatt cgggagagca ctggaggagg agagcgccgc cggtggaaaa
     4381 gccgtcgatc cggtcctgca cagcgagaga aagagccgga caaacaaccg caaacacaag
     4441 aggcttgagg ccgtagttca ctccctgatc gcctcaagca cggcggctga aaggcgctcg
     4501 atctcgtcga atcgcggttc ggccatcccg ttcttctcga tgccgcagtc agtcgcgaca
     4561 aggtgcaggt gcggctcgat tccgagcgcc cgcaccttct tccgcccgca acagtcgccg
     4621 cacccgtcca gcaccaggat acggtccgca taccggatcg cactctccag cgacgcggtc
     4681 ggccgggtgg ccgcaaggca cgcctcgacc atcccgccgt agcggcgcat gagcgccgca
     4741 ccggcctggg tggtcagctt tccggtgttc gagatcccgg aacaggtgat gacggtgacc
     4801 gccatcgtca cgcttctccg aacgtcgtat cgatccagcc gacaatctcg tcccggatct
     4861 gccggacgcc ggccatgatc tcctcgtcgg tgccggtgag gttcgacggg tcgggaaact
     4921 cccggtggat ctcctccttc gcccagggga agagggggca gatcccgccc tcgcagacgg
     4981 cgacgaggag gtccatctcc tgctcgtcga actcaccgag atcctttgag cggtggccgg
     5041 agatgtcgat cccgatctcg gccatggctc tgacggcata ggggttcagg gttccaggta
     5101 ccgtgccggc cgagaaggcc tcatagcggt cgccgtagcg ggcgttcagg tagccttccg
     5161 ccatctgcga gcgggcggcg ttatgggtgc agacgaagag gactttcttc ttcatggtat
     5221 cactccagcc cgagcgccct gagtgccgcc cgggcgatca gctccggcgg gatcgactcg
     5281 tagcgctcgc cgtcatactc gaccgcacgg cacgctgcat cgtcctgccc ggtgatgcag
     5341 gcgcaggagg cgcagggcgt ggtggtaact tccataccct cgatcaggtc ctcaagcggc
     5401 atgccgttga agaggatgct gttcgactct ttgatcgccg cgttctcgat gggcgtctcg
     5461 acaacccgga ccgtgatgcc ctcctcctcg aggatggggc ggatctgctc gacgacgtcc
     5521 atcacggcct ttcccgtctc gccgcaccgc tcgcaggtgt gctcgatgtc tcttccgata
     5581 tgccgccatt caacgacgag gatgtccgtc ggccccccgc agccgcagcc gcatccaccg
     5641 gacggcctgc gctcgttctc ttcctctgct ctccccttcc cctcccaggc ggcggagacg
     5701 accgtctcga cgtcctcttc ggaaaggtcg aggcttccgc ccttctcgat cccgagagcc
     5761 gtgaccacga tctcctggtc gggcgcaatc ccggcccgct cgacgatctg gcgggcgcag
     5821 ccgaccgggc agccgtcgat gacgaccacc tcatcggcct cctcgacttt cttctttatc
     5881 gagggtgtag agacggcgag agatgccgtg caggcgatat tcccgtatcc ttccttcgtc
     5941 aaccaaacgg cagcctcgtt cgagagctgt ccggccttcg acgcgccggc gaagatgatc
     6001 cgtttcgtgc cgccttccgg ctccgcgcca cccccgcacg tacaccgggg cactgtctgt
     6061 tctgtcatgt cacttctctc cgagcagtat ctgtttgata tcctcgacac aggggacgtg
     6121 gccgacgact ttaggctcgc cgtcgacgta gagcgccggc gtcagcatca ccccccgttc
     6181 gatcatcgta tcgagatctt cgatcttgtg gatgtccgca tctattttga gatccctgac
     6241 tgcgatctcg acgttcttgt tcatccgctt gcacttgacg cagccggtcc cgaatacttc
     6301 tatcagcacc attcttcctc cgcgcgggga agggcggctt acccctgcag gatctccttg
     6361 atctctttta cgtcggccac ccggccggag accttcagtt ccccatcgac ggcgagggcg
     6421 ggggtcagca tcacgccgcg gtccatgatc tcggtgatat cgtcgacctt gacgagttcg
     6481 gcctcgatgc cgagatcttt gatcgctgtc tctacgttct ttgcgagccg cttgcatttg
     6541 gcgcagccgg ttccgagcac ttcaaccttc atcgtttctt cacctcgttc tgatacgcta
     6601 aaaggagcgc cgcccggtag agcggctccg cctccttcgg gatatagttg taaatcttta
     6661 tctgtctcgc cagttcgtcg ccgatcacgg taacgcccgc gatgccgcgc ttgatgacgt
     6721 cggccaggac gtcgtcgagc atcgcgagcc cgacgacgac ctcgccgacc tcgatccgtc
     6781 gaatgcgccg cagggcttcg gcggcgcagc agggtttctc tctcttcttc gtctccatac
     6841 gatcactcct atatgagcag catcccgttg ccgtagacaa acccggccat ggtcgcgaaa
     6901 gtgaccacca gcgccgtgta ggcagccgtc ttcttcagcc cgacaatacg ggagatgacg
     6961 aggacggagg ggaggctggc cgtcggcccg gccagcagga gggcgagcgc cggtcctgca
     7021 gccatgtgcc ccgtggtgta gccgaacgtc gtgccgatga tcgggacctc gagcagcgtc
     7081 ggcatgtaga ggatcgtccc cacgatcgcc gcgaggaagt tcgcgccgag cgagttgtcc
     7141 ccgaagtagg gctggaaggt ctcgggcggg aggaagaagg cgatgatccc gacgaggaac
     7201 gttcccccga tcaggatggg aaagatcttc ttcgtgagat cccaggtctc gagcccccag
     7261 tcggtcacct cgtccttatc gtagtagtag atgaggagga cggcgatcgc gagggtcagg
     7321 acgtagacga tcgagagccg gatcacccat tcgagcggcg aggcgccgaa gaccagcact
     7381 ccgaccagga gagcgaaaaa tgcaggcgtg acccagcgtg gtttctcttc ctcgcacgcc
     7441 ccggcgacgc tcggcgccat cgccttcttc ttgagcgtnn tcaacatccg tcgaccggaa
     7501 gatcagcgcc ataagaagcc cgatgacgat cgcgagcacg accgcagaga ccgcccgtgc
     7561 tatcccaagg tcgaacccga ggaccctggc ggtgtagatg atggcgagga tgttgatggc
     7621 cgggcctgca aagaggaacg tcgttgccgg gccgatgccg ctccctttct tgaggatccc
     7681 ggcaaagatc gggaggatgg tgc
//
LOCUS       DAJB01000244            5168 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_172494, whole genome shotgun sequence.
ACCESSION   DAJB01000244 DAJB01000000
VERSION     DAJB01000244.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_172494"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1160 a         1434 c         1443 g         1129 t
ORIGIN      
        1 caagagggta tcgccaggaa ctatactatg accaggaaac cccgcgatgg cactaccctg
       61 ttgttctcat gctcaaactc ctggtcatca agtgctttca ccaacaatct tacgcccgcg
      121 ccatctactc ccttactcag gaagattggc acaatctcgg cgtatccccg gaagatcggc
      181 taccccaccc tgcaacgctc cagcactttg tgaaataccg tcttggagtg gatggtatgc
      241 agcgagtcat gatgctgacc ggtagcgcgc ttacgcgcaa actcgacggt gagtcgattg
      301 gcatcatcga ttccactccc ctagaagcat cgcggtataa ccaggatgcc ccgtacaatc
      361 cccactatca gataaagatg gataaagcac atatcatgca tctgggtcgt tatccactgg
      421 cgatggtgta ttcgggagga acagatgccg atatcacgca cctctcccga ctcatcgcaa
      481 ccgttgcacc aatgcaacca aacgtggtag gtgtacaact tgacagcgca tacgattcct
      541 atgaggcaca tgcacagatc tggtattacc tggatgcatg gccgtgtatc gctctccgcg
      601 agggggcggt cgtacaggag gagggtaccg agaaacggat ccatcactgg gtgaataaac
      661 tctggaaaga agggggagac gtacatgccc ccctggaaga acagctaaga ttcctcttcg
      721 agcagggaag aggagagcag gttgggatgt tctttcggaa taagaacacc ctcaatccag
      781 aattctcaac gctcctacac ggccgcggtg attgcgagcg cgtgcacaac cacatcaaga
      841 caactgtcgg gttccgcctc aaaggaatcc gacatgagag tcgagaactc tacatcctcc
      901 tcaacttcat cgtatatcaa cttctcctcc tggtcgggtg tacggcagag atcgagaacc
      961 caagtgtgct gagtgcgttg atctgagcga aaatgctcga aagaaaagac cggtctggca
     1021 aaaagagcgg agcaaccggt tcttgggaga ggttccgttc ggttaagtga acggaggata
     1081 tttttggaat gagagaggaa aaagcaggaa tcaaggaaaa aaccaaaatg ggtacctgaa
     1141 tagactaacg gggacaagtg cgccgaaaag catgccatca aatgagggta ctctctgatt
     1201 tgaagggctc tccaccgacc acgtggttgg acctcccccg gcatttctgg cctccttctg
     1261 accgggtctg gatacttcct ctgaccggct ctgttctgcc cccgggataa ccgtcacttc
     1321 ggggatcctt cagcgctgag tttttttgag atcgatgggc ccgtcgagat ttgaactcga
     1381 tgtctccacc gtgtgaaggc gaattctgcc ctagtttcca attaagggcc caaatattcc
     1441 atatcttccc cgacacttcc ggtggaaaat ccgcgtcacc tacgcctgta gagccgtacc
     1501 atccgcctct tccgtagcga ttggctttcg cgatgccgct gtgccgtctt tccgagcata
     1561 tagggcataa tggttaggtt aatagaagca tcgcaccaag gaatgcacta aggggcgatc
     1621 cggtatgcag gaagaggtgg aggagacggc agggcctgat cagacgccgg agttcgtcat
     1681 cctgcgtgtt gcgctctact cccttgccgt caatgccggg ctggtcgtcg caaaactggg
     1741 actctcggtc tattcgggga gccttgccct ccgtgcggac ggcatccact cgttcgtgga
     1801 cgtggtcgcc tcgcttgcgc tgatcgttgg ggtgtatctc tcctcacgca aacgccggga
     1861 tttcccgtac ggtctctaca aggtcgagaa cgtcgtctct gtagccattg ctctcctcat
     1921 cttcttcact gccttcgaga ttgcggcgga ggcggtgacg ggcgatgccg ccgccctgcc
     1981 ctacagcggg tggctcctcc tcgccgtcgc cgccttcatc ccggtgccgt acatcctcgg
     2041 cacatacgag gtgaacgtag ggcagaaata caactctccc agccttatcg cggacgggag
     2101 gcagcaccgg gtggatgtcc tctcctcctc gattgtgttc tttgcgctcc tcgggcagtt
     2161 cttcggcata cccctggacc ggatcgcggc cgtcattgtc gccgtcttca tcgtccgtgc
     2221 gggctggggg atcttaaaag acagcatgcg gaccctcctg gacgcctcgg ttgattacaa
     2281 tacgctcaac gagatccgca caatcatcca atcggacccg atggtgagca gcatcaagag
     2341 tatcacgggc cgcaactccg ggcgctacat cttcgtggag gcgatggtcg agatggcgga
     2401 gtcggatttt gagaaggcgc accgggagag cgagcgcatc gagcagaata tacggcagca
     2461 ggtgccgaat gtcgagcggg tcctcatcca ctacgaaccc gagaaaaaga cgcacctgcg
     2521 gtatgcggtg ccgttgcagg accgggaggg ttcggtcagc gttcacttcg gcgaagcgcc
     2581 gtatttcgcg atcctcgact ttgacattga gaagaaccgc atcgaacggc aggatatcat
     2641 cgccaaccct tttgtggatg tggagaagcg gaagggggga caggtggcgg agatgctgct
     2701 cgcgcacgag atcgatgtgg tcttcgttca tcactcgctc tcgggaagag cggccggcta
     2761 cgcgcttgag gcggcggggg tcgagatgag ggcgacctcc gcggcaacgc ttgcggaagt
     2821 gatcgacgat gtggtcgtga atcatgggga ggagaccgac gcttcgcctg gctgatccct
     2881 cgtcgcatgc ccggctgaca cggcgactgc cggatatagt ctcaagttcc ttcgcaatag
     2941 agaaattatt ttaaccggta tcgaagatcg cgttcgatcg agcatgaagg caagacaaat
     3001 agttggagca cttctcggcg catttatagc atctctcggc cttttatggt ttctgcaagg
     3061 cacaggaatc cttcggatgc gccccagact atgtattgca aactgtgagg agattgtcgg
     3121 cccatctccg ttctgggcag tatcaggggt gatcgctctc attattgggg ctattgtcgt
     3181 tttcgtaagt gtgagaggcg tcagaacacc ctgagaccct gatcgatcgg actgccttgc
     3241 cgtccattct gcgatatggg cacgacccgg gcggttgcat tttttgccaa aaaaccttta
     3301 tctccgccga aatcccacaa aggtatcaac agagggtccc gggagcatta tgcatcacca
     3361 caaaaacatc atcctcatcg gcatgcccgg tgcagggaag agcaccgtgg gcgtcatcct
     3421 tgcaaaatcc ctcggcatcc agttcatcga tacggacatc ctgatacagg aacgggccgg
     3481 gaagatgctc caggagatcc ttgacgagga cgggccggat gcgttcaagc ggatcgagga
     3541 agagacgatc ctctcccttc atccccgcca tgccgtgatc gcgacgggcg gcagcgtggt
     3601 ctgcagcccc gatgcgatgg cgcacctgaa gtcggcaggg gtggtcgtgt acctcgaggt
     3661 ctcgtatgac gagatggcaa agaggctcaa gaacatcacg acccggggga tagtcctcct
     3721 tccgggccag agtctccgcg ggatgtacga cgagcgcgtc ccgctgtacg agaagtatgc
     3781 cgatctcacc gtcgcgtgtt cggatgagga tctcgagtct gtggtcnngg gggagtctca
     3841 ttcgctacga tcctctgcga ccaggaatcg cccttcctca tattcggcga agcgcaccgt
     3901 gagcatttcc ccctcatgca cgccgaaatc cgactcgggt tcattcagga gcgttccccg
     3961 gaatgacgtg tcgtcagccg actggtcttc aaggcggacc cacactcctt cagggatcgt
     4021 ttggtggtct tttgagtaaa ggatcaccga tacatcgtca aaatacccgg gagccctgaa
     4081 cctgtcgaga tcaaccctgt ttcttatcgt gcggagcgat tccggttcat aatagatgcg
     4141 ccatctctgc tcttcgagta acgagagact gtttgcttct tcattcgaga ggagcgtata
     4201 cgccccgact tcatcggagt ggagtatgag cccctcgccg tgatcctcga agttcgcgac
     4261 aatctccggc ggtcctccgg gctcggtccg gcacagcgta tggatacgaa aggtgatgcc
     4321 ctcctcgtgg tccacataaa aaaagccgat gcaccgatcg tgcgtccggt gcatcacacc
     4381 cttcacggag aggatacgag agatgccggg accgatctct gcaaccgccc accggttgac
     4441 tatctcccgg tccgaatggc gtgtcattcc atcgttcatg gtatgtggta cagggtcttc
     4501 atgtgcaatg tgaaagcggt ttcgaaatgt tcatggaaag tctctgatta gctctctctg
     4561 tcaataagaa gtatatgagt tgaggaaggg cccgataccg cccccgtccg ggcatcgaga
     4621 tccctcgtat atcgcccaga tcttaagtgc atcgttgccg ccatgctccg cgacggtatc
     4681 cctcgccgtc agatcgtcga ggagacctcc gcgctgtacg gcatccggcg gggtgtgtgg
     4741 aagaggtagt ggccgcggtc ccggcgtgaa agtgtcgcgg tcccccaaga gtgcgtagag
     4801 gaagttatct cagcctgtcc cccccaataa gccccttttc acctaccccg tcaacccttt
     4861 ttcaaacaat tggcaccccc cctttatctc cccctaagca gagtctcaga tacgcgaata
     4921 tgtccggatg tgtccggaca tgtccggaca ggagaaaaga accatggaaa cgagagaaaa
     4981 gaagaatccg acgataatcc atcggattag aaaaagggtc cacactactg tatcaccgga
     5041 gacctacgag tatctaaaga atacagccgt taacgtgggc caattggtag atagggcggt
     5101 ttctgaactc cgaaaggtaa cgcctcacgg attggttctt atttcgcaaa agaaggaaga
     5161 attatggg
//
LOCUS       DAJB01000245            2809 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_87318, whole genome shotgun sequence.
ACCESSION   DAJB01000245 DAJB01000000
VERSION     DAJB01000245.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_87318"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          567 a          933 c          802 g          505 t
ORIGIN      
        1 cctccagttt tctccccgga ggcaggagag gcacatgttt aagtacgcag aatgcttacc
       61 ttccctttgc ctccgggctt gcggggtgcg tcatccgggg cgtcgacctg gggatgggcg
      121 gcccccgcac acaagcgggc accgcacgcg gcacccgatg cgattctggg tcgttagaac
      181 atataaaatc agcaggcatc gttcggctgg aaccgaacgc acgataaacc ttcttattgc
      241 agaacgatta tccaacctgt gtctccctgc cccaaagata gatccaccga taccccgcca
      301 ccccggacgc agggttatgg cggggttcta tctggcatca gtacttcatg tacatcccga
      361 gcaccgccga tccgccatcg gacgtcggga tgagggacac gaccctgctg tactcgtcca
      421 gccgctgcgt cgacgtccat gacgcggctc cgtcttcatc gaacccctcc acccggagca
      481 gggcaccgcc gtagtcgttt tcggcaagnn gccggaggat gcttccctcc tcgccgagcg
      541 agacctccag aactcccgcc ggttccgcac ccgtgacccc ggcaagcagg ctgtaaccgc
      601 cggccgcctc ctgccgcgcc gccgagagga ggtaccggtc cccggggccg ccgcggatcg
      661 tcgtgttcca gacctgggcg ccccggttgt cgaacttcgc cgctattacg gctccgggcg
      721 cccctgcagc ttctccggcg atgagggacc ctcccgccgg ctccgggacg acgatcggga
      781 ctgaacccgg actcacgccc ccggccggct cctgccaggc gacggtgccg ctgccgtcaa
      841 tcctcgcaac ccggtcgtca ccggccgcga tccggcccgt atcgcccgag ggcgcgatcg
      901 cccagtactc cccctccgcg gcaaggtccg tgcggtgccg ttcgctccct tctctgtccg
      961 tcaggatgac gaggccgcag gaggccgcga gggcgaactc ccccagaccg gcagatgcga
     1021 tccccgtccc gcggcggtcg cccggagccg ggtaggtcat accccatgcc accgccccct
     1081 cccggtcgag gaggattgcc ctcggcgtcg ggtggaagtc acctgtctcc gagaccgaac
     1141 cggcgaccgc gtagccgccg gggacctcga taatggagaa cgcttcgtcg aacctctcgc
     1201 agtcgatgac ggtgaaccac tcccggtccc cggcatcgtc cagtttcagc acccagacgt
     1261 ccccctccag cgtgcgatgg cccggcgtca gcggccgatc gaagcacccg gcggcgacgg
     1321 ccagcaggat gagcgcggca accactgcca aggtccagag tcttcttttg gttggtgtca
     1381 tcgcttcatt cccccggtgc accttcaccc aggctacgta ttacgatgac cgcaatcagg
     1441 cagacgaccg ccgcaccgaa gaatacctcc aggtacagcc cggccagcag accgagagag
     1501 aagaaacccc tatacccaat ctccccggcg gttccgatcg cttctgcaag ggcgtcgaag
     1561 agtataccca ggaccggcag tttttccgcg aagaccggcg gggcggcgcc gggcaattta
     1621 tcgggaccca cgagctgggc aaccaggaac ggcagctgaa gcagcgatcc aactgcaaca
     1681 cacaacctcc tgccgctcgt agcaacattc ccgaacagaa aatgcgccgt cgccacggcg
     1741 gatgccgaaa ccataaccat cgagagcagg attatcccgg atatcagcag gctggaggga
     1801 gagcctgcat aaatcgtacc gatcgtgccg gatgcaatgg cagcgatacc tatcccgagc
     1861 gaaaccgccg ggcgccgctt ctcctgtccc agcttcccga ccagggttat aagagcgaac
     1921 ggccacgcga ggacgaaggc gacggccgga aagaagagca cgctcacggt gcatccttcc
     1981 cgcgggacct ccggatgagg acgtacccca acgcgaggac gatcacggct cccaggccgc
     2041 atccgaggag cacgggggtg tagaagatgc tgaatgcact ttcggagccg tgctacgttg
     2101 tcgtatctgc gggctccgca agcccggttt gccgggcatt ctctgtatca accagcaatg
     2161 cctcaccgtt tgtcgggtcg atctggatgc cttcatccgg atccgcggct gcatgcgcca
     2221 caccgctgcc ggccaggatg aagagcagca gggcaacgca gatcagtcct cctcgaccgg
     2281 taccgttcat aaatcgcctt ttgttcatcg atttttcacc tgccgaccta tgccgcgatg
     2341 ccgatccagg tatctacgat ggagacgtcg acgctcttct ataaaccttt tctgtgacgc
     2401 ggatttacaa gagcagtatg atgcaagggg cccggatagc agtatgaggg cccattcttc
     2461 tgcactcgtc caatgtttca acgcggaccc tccaggaggg atcctaccgt tccagaggta
     2521 gaactccggt tcacgcctgg cagaaacgat agggagagcc atttatcgga ggaggtgcca
     2581 agctgaatat tgccgtacat atcaacggtt atccaggaat caactgagaa ttttgcattt
     2641 gttgggaaat tgtacaacga ccagatgtca ttattagtta cccagccagc gatgttacta
     2701 tgtccgactt ctgagtggca gtcctggtgc tggtaaaaac cagttggagt aactgcatat
     2761 gatctgtcag tgacatggag cacagcccgc ccataatcta catagaacc
//
LOCUS       DAJB01000246            4961 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_45942, whole genome shotgun sequence.
ACCESSION   DAJB01000246 DAJB01000000
VERSION     DAJB01000246.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_45942"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1022 a         1570 c         1448 g          917 t
ORIGIN      
        1 ctctcgggcg ggatgaaacg gcgcctgatc atcgcccggg ccctgatcaa tacaccggaa
       61 ctgctcatcc tcgacgaacc caccatcggg ctcgacccgc aggcacggca cctgatctgg
      121 gagacgctcc ggaacctcca ggcagaagga aacaccctgg tcctgaccac gcattacctc
      181 gacgaagcgg agcgcctctg cgaccggctg gtgatcatgg ataacgggag gatcctcgtc
      241 gagggcgccc cggcagacct catccgcgag catgccggga gcgatgtcgt cgaagtcgag
      301 cggacgccta aggtgatcgc ccgcctgacg gagctcggtg tgaactacga tcccgccggc
      361 ggtctgctcc agatcttcac ggaccgtccc cacgacgtgg cgcgcgaact gctcgaggtc
      421 tgccggcacg aggcggcggt gacggtccgc ccggcaaccc ttgaggacgt ctttctgcgg
      481 ctgaccggcc ggagactgcg ggagtgagcg agcgatgctc gacatctccg gcagggtctg
      541 gagcgtctgg cggaggaact gggacgcctt cctcaggacc taccgggtaa acttcatccc
      601 gcccttcgtc gaaccggtcc tctacctcct ggcgctgggg ttcggcctcg ggacgtatat
      661 cgaggccgtc gacggcatac cctatcccgt cttcatcgca ccggcgcttg tctcgatatc
      721 ggtgatgtat tcggccttct ttgagtgcac ctactcgtcg ttcgtccgga tgtactacca
      781 gaagacgttt gatgccatca ttgcaacgcc ggtcagcatc gatgaggtga tcgccgggga
      841 gatgctctgg ggcgccaccc gggggatgat ctacgcgaca ctgatgctcc cggtgctcct
      901 cctctttaat gtcgtgacca tgccgtcgtc cctgctcctc atccccttcg cgttccttgc
      961 cggactcctc tttgcaagca tcgcgatgtg tttcacggcg atcaccccga gcatcgacgc
     1021 gctgaactac ccatccttcc tcttcatcac gccgatgttc ctcttctcgg ggacgttctt
     1081 cccgctcgac ctgctcccgc agccgatcca gtacttcgcc cttgcggcgc tcccgctcac
     1141 gcacgttgtc agcatcaacc gggcgatcac gctctcggcg ttttcgccgt tcaatctctt
     1201 taacctcgcc tggatcgtgg tggtgactgc gctcttcttc atccttgcta tcagactgat
     1261 gaggaggcgg cttatcgtgt gaccgctccg cgcggccggc cgataacgta gagcagatgc
     1321 tgcgggcagc gcaattacga aagcgcacca tacgggcgcg tgcaaaaaaa ggttagaatc
     1381 tgggttttct gtacttcggg aggcagtcac ggcaatagac aggccgaccc tcggtaggct
     1441 tgaaaggaac ttcgcactct ttgccgcagt cggaacaaac ggtctttgtc atttcgcggg
     1501 ggcggtcgtc gaacgaacgg gggcccctgc ttggatatct ctcttccatt ggaattcact
     1561 cactttgttg gattatattc acagacgact cagtaacggg gtttccggtg cttcgggagg
     1621 cagtcgaggc agtagacagg ccgaccctcc gtgggcttga aaggaatctc gcactcttta
     1681 ccgcagtcgg aacagactgc cttgtggaac tcgcgggggc gatcctggaa cgagcgggga
     1741 ccccggctcg gatatctctc atccatatac ttcaaccatg cagatagtct gcatatacac
     1801 aactacctgc cggcatatat aggtgcgtct cgcagatcac gggcagcaaa aattccccgc
     1861 gtcggcccgc cttgcccggc cggggttgtg caccggacgg cacaggaccc tgcatccccg
     1921 gtgcgtgacc gggtctcatc cagcaagaaa atcccctgca atgcggctgt ttatcccggc
     1981 agggagaact gtgacacaac gcttttatcg atactatcct accaaatgat catccaacta
     2041 tggcagacga tgaccaatct cttttgcaat ccggcaaaac gccggttcta tcccggagac
     2101 gggagtaggg ggaacggctc agcatgaccg acgatattca gaccgacgaa ccccctgata
     2161 gcagaactaa aggaaaacac cgggacagga ggacgatcgg tccggtcccg aaccttgaag
     2221 agtatgtgaa acccgactgg tggaggggca tcttcaaccg cctctactta aagaccgacg
     2281 gcgatgtcgt cgacgatcag catatcaccg agagtgagat cgacaggatc gttcaggtct
     2341 taaacctcca gtccgatgcg aagatcctcg acctctgctg cggccagggc aggcacaccc
     2401 tcgaactcat gcgcaggggc tacaacgccg aaggcctcga ccagtcacac tacctgatcc
     2461 agcgggcacg gtcgaccgcg aagaaggagg ggctctcggt ccggttccgg gagggagacg
     2521 cgcgccgtct gccgtaccgc accgacacct acgacgtcgt cctcgtcctc gggaacagct
     2581 tcggctactt tgattccgtt gaggaagacc tgcggatcct caccgaactc cggcgcatcc
     2641 tcaaaccctg ggggcgggtg ctcctggatg tggccgacgg cgattactta aaagagcact
     2701 ttcaggcgag gtcgtgggag tggatcgacg acgcgatgtt cgtctgccgc gagaggtccc
     2761 tctcgctcga tgaacagcgc ctgatatccc gggaagtgat caccgatgtc gagaagggcg
     2821 tcgtagccga ccagttctat gccgaacgcc tctacacccc cgaggcactc cggcggctcc
     2881 ttgagaaggc gggcttctcc gggatcacct tccacaagat cgacaccgag tcgcagcgga
     2941 accaggacct cggcatgatg gaacgccgcc acatcgtcac cgcggtcctc aagaaggagt
     3001 ggtcgacgac gaagacgaga ggtcaggaga gagcaaaaca cgtcgcggtg atgctcggcg
     3061 acccggccaa gcccgacgcc ttaaaacctt cctgcacctt cgacgacgac gacttctaca
     3121 ccatcgacca gatgaaggcg gccctccggg aactgccggg gtaccggttc acgttcctct
     3181 gcaagcacga cacgatgatc caggacctcc cgaaactgaa ggggaaggtg gactacgtct
     3241 tcaacctctg cgacgagggt ttcaacaatg acccgaggaa agaactccac gtcccggcgc
     3301 tcctggagat cttcgagatc ccgtacacgg ggtccgggcc gcagtcgctt gcgttctgct
     3361 acgacaagtc gctcgtgcgc ggcgtcgcga aagagatggg gatcccggtg cctgacgcct
     3421 gctttatcac tcccggcgac cgcacctacg atgtcgggat gaaactcccg gcaatcgtca
     3481 agccaaacgc aggcgactcc tcgtacggca tcacgcagga gagcatcgcc cgcaccatcg
     3541 aagagctctc cgatgtcatc aacgccctgc ggacgaagtt aggatacgac cgctcgctcc
     3601 ttgtcgagga gttcctcacg ggaaaggaca tcagcgtcgg catcatcggg aacccgtcgg
     3661 gatactgcac ggtgcttcct atcatcgagg aggactactc cgcgctgccc ccggaacttc
     3721 cccggatctg cggctacgaa gcaaagtggc tcccggactc gccgtactgg aagatcgtat
     3781 cgaagccggc ggagctcccc gaggagatcg agaaggtgat cgtgcggtgc tgtctcgcac
     3841 tcttcaagcg cctggagtgc cgcgactact gccggtttga ctggcgcctg gacgagcacg
     3901 gcagtccgaa actccttgag gtcaacccga accccggctg gtgctgggac ggccacctcg
     3961 caaagatggc aaaatacgca ggtctctcgt accccgagat gctcggccgg atcctcaaag
     4021 ccgcggaaga acggtttggt atggagcccg agaccggtgc gcgggagggc gagaaggatc
     4081 ctctcccgtt cgacctcacc cggcagacag cctgacccac ccaccttttg agcggagcgg
     4141 gaacgatcct gcccgacatc ccccgtctcc ctgaccggag ccgcgacgcg ccccagacac
     4201 ccgggccgga gagcccggtg ggcagcgcac ggaaccggtc gagcggacca tgcgatatat
     4261 gagtgccgcc tgccggccgt tacccatcag tatataatat cctctggcgc atagatctga
     4321 ttacatggcc gatgaatcgt atcggactgc aacggtccag ggcagggagg tcccctacga
     4381 tccggagcag atgaggaagg tcaacgagca cccgtgctac tccgagaaag cgtgccacgc
     4441 cttcgggcgg tgtcacctcc ccgtcgctcc gaagtgcaan natccagtgc aactactgca
     4501 tacgggactt cgactgcgtg aacgagagcc ggccgggcgt gacgagcaag gttctcaccc
     4561 ccgaggaagg cctcgacctg gtcaggaagg tcgtcaaaga ctacccgttt gtgaaggtga
     4621 tcggcatcgc agggccgggg gagccgctcg caaaccccga gacgttcgag gcgttgcggc
     4681 tggtccacga ggagtttccg cacctgatca tgtgcctcag cacgaacggc ctcatgctcc
     4741 cggagtcgat cgaggaactg gcaaagtaca atgtcgggaa tattacggtc acgctcaacg
     4801 ccgtcgaccc tgcgatcggg gagaagatct actcnngaag acgttcgtca agataaacac
     4861 cgtctacatc cccggggtca acgacgagca tatccccgag atcgcaaaga aggccggcga
     4921 gatgggggcg ttcaccttca acgtcatccc gctcataccc c
//
LOCUS       DAJB01000247            9032 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_77325, whole genome shotgun sequence.
ACCESSION   DAJB01000247 DAJB01000000
VERSION     DAJB01000247.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_77325"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    8618..8693
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1950 a         2641 c         2525 g         1836 t
ORIGIN      
        1 tagcgcccgc ccccacacgc caaaattcct aacataaagt gacaagatgc agtaggtgct
       61 gtccggcaga gagtattcag ggaacctcaa cccgcaatct gcccgggtcc tcggagacgt
      121 cttccgcagg ccggcgaagc agaaggcgta tctcctggac aaccctctct ccttccgggg
      181 tatcctcgag gatgaagagg atcgaacccg tcaacaggac gccgatacca agcaggatga
      241 tcggcggtgc gagggtgagg agcatcgcca cgcaggatgc ggcgcccagc agcggaaaga
      301 ggatgccgaa cggaacggaa aaataactct tatccggggg aagcagagcc ctccgcctca
      361 ccagaatgat ggcgcttgtg ttcacgagcg tcatggtggt gaggatcgtg acattggtga
      421 gttctacaat aaaggagaga tccccgatca gaatgaggag gagcacaaac ccggccccga
      481 gcaggacgga gttgatcggc tggccgttcc agatccgggc aaatcttccc gggatctgtt
      541 tttcgctcgc catttccagg attattctcg aggccccgat gatgttggca ttcgcagacg
      601 agagcgtcga gaagagtgcc cccagtgcaa cgataatccc gccgacggat ccgaagagga
      661 caaccgatgc atcgaagaca ctctcgctcc cgtacgaggg aagccgtgcc gcgagcagcg
      721 cgatgatgac tcccgtatat atcagcgcca cgatgccgat ggatgcaaga gtggcaagcg
      781 gcacgttttt cgaggaactc ttcacctctc cacccatcat ggccacgacc tgaaacccga
      841 tataggcaaa aaagatcatc gccactccct gcagcatgcc gcccacgcct cccgggagga
      901 gcggcgtgaa gtcggaggcc tggatgtgaa cgagcccgaa cagtatcagg gccccgagga
      961 tcgccacttt cgaggcgacg agggcgactt cgatcttccc tgcttccgcg atcccccgga
     1021 ggttgagggc cgtgagcagc acgagggcgg cgatggcgcc gtaccgcgga tcaaggttca
     1081 ttccggtcag gaggtttgcg tagatcccga acccgaggag aacaaaggca gtggcgatgg
     1141 tgacgctgat atacatcgcc catcccgcga gaaaacccgg atagggccca aggatgcgcc
     1201 gggcgtagag atatccgccc ccgtcctcgg gataaatgct ggcgagcgct gcgtaggaga
     1261 ggcccgagaa gacaacgatg agaccgcaga gaaggtagga gaagactgcc gaagggccgg
     1321 cgatacccac gatgaggccg ctcagcacaa aaaccccggc gcccaccata tttcctaccg
     1381 caaacgcgat ggcagcgtat atgctcaacc ctttggttcg atgtgctccg ttctgcattg
     1441 tgttccccct ttcggcnnag agtatgcaaa aagattactg acagcagttc ccgccgtgcc
     1501 tgtatatgcc tgaacagagg tacgaaccgc cgggcgggaa tgtctaatac cgggcggggt
     1561 ttttcgtcgg catcggtggg acggataatc cctgccgtgc ccttctggag caacggagga
     1621 actgcaggat cgccattgag tttatccggt atttttccgg aagatgggcc ggagggagcg
     1681 gcagggaggg tgccgcccgt ccctgttcag cggctgcgct tacccccgcc gccgtgtcct
     1741 gccgcatctg cggcggcagc gtggtgcgga ctgaccgtgc cgacgtatcc tgcgtgagcg
     1801 agtgaaacgg cggcgttgat gatcccgatg tgcgagaacg actgcgggaa gttacccata
     1861 tgggttccgg tcagcggctg gagctcttca gcgtagaggc cgaggggact ggcgcgtgca
     1921 aggaggttcc tgaatatccg gtgcgcctct ttcttgcgcc cggcacgtat cagcgcgttg
     1981 acaagccagg tcgtgcagac gacaaaagac ccctcctttc cggtcacgcc ttccggcgcg
     2041 tcgacgtagc gataacagag gccatcgaca acgaggcact tgagcgtggc gtctatcgtc
     2101 aagagcatcc ggggatcgtc tccctcaatg aaattgatgg tagagaggcg caggttcgcg
     2161 gcatcgagct gctcatcttc gtaagactgt ttgaacgcgc ccaatttatc gctccacccc
     2221 ttatctaata cttcctcccg gatctggtca cgttcgcgtc gccagtcttc aacatggccc
     2281 gggagccgca taccttccgc aatctcgatt ccgcagttga gagcgaacca catcatggct
     2341 ttactgtaaa cgaaatgccg cacgccgccc cggacctccc agatcccgct accccgatca
     2401 tgccagtgat cggtcaccca atcgagcatc tgccgtaccg tctcccagat cggtgtcgga
     2461 tcgtacttcc cgatccgcca ggcgaactgt attgcaccca taacctctcc gtagacgtcg
     2521 agctgttcct ggatcgccgc cccgttcccg attctcaccg gtcgggagtc ccggtaaccc
     2581 cggaaattgt cgagtatctc ttcggtggtg ctgctctccg ggacaatcgg gtacaggatc
     2641 gagatacccg ttcctttcaa ttcgaccgta cgcacaagcc agtcgaagaa cggccgttca
     2701 tcatcgaggt aaccagcaag gagcagcgca taaagcgtga aagacgcatc gcgaatccag
     2761 gtgaagcggt aatcccagtt ccgctcacca ccgaaggatt ccgggagcga tgtcgtcggt
     2821 gcggcaacga tagcgccgga cggatcgtag gtcatcaact gaagggtgag agcgctgcgg
     2881 attacggcat cgcggtacgg tccgcggtac gtgcacttct cgctccattt ccgccagtaa
     2941 tcggtcgtca cgttcagggc atctgccgga tcaagctgcg gcagggcagc gtcgtcctct
     3001 gtcacgagtg tgaaagccgc cgtttcaccg gcgtgaagca tgatccggca ggtcagcgcc
     3061 ttgttcacat cgtctacctg ccatgccgtc ggtccggtga tcgtgcaggt cccgaatgtc
     3121 acccgattcc cgtgaggtgc aaactccggg agtgtgcggg cgtaattcgg acggggcaga
     3181 caatcgcaga caatcgctac cttcccctcg gtgcattcca ctatgcgtac aatcctgccg
     3241 ggagccggaa gccggcggaa gcgggcaacg cttgcaatat ccatgaaatc ccgcaggact
     3301 acagttccgg cagcgcaccg gaacgtcgtt tcgaggacgt tcgttccttc acgatagcga
     3361 tgcgaactct cgaaacgccc ttccggctgt atgctccagt agccgccacg atccggatcg
     3421 agaatacggg caaagagcga cggtgaatcg aaccgatgga aacagagcca gtcgacggac
     3481 ccacagctgc tgacgagcgc cgcggtatgc ccgttcccga tcaccccgta gtcttcgatt
     3541 ggccggtact catcatggga cgcgccttca cggcgagggg gctgcatggg aatttgtgac
     3601 cggtttccct ggatagtatc cgacatagca atcctattat tgttttggta cgcccttcag
     3661 ctcacgagga tgtcgaccgg ctctgaaatg ttcttcgatc tcttcaagtg tgagtccttt
     3721 tgtctccggc accagcctga ggacgaagaa ccatgcgaca atgcccacga gtgcgtagag
     3781 ccagaagacg cctgcccgtc ccagtacccc ggccagtgtc aggaatgtca gggtgatgag
     3841 aaagttcgca gcccagttgg cgatggttgc gacgctcatg gcacgccctc gtacattgag
     3901 cgggtagatc tcagcgatca gcagccagaa gaccggtcca aggccgatgg caaacgaggc
     3961 gacgtatgcc atcaggctga ccgcggtaat cagccccagg aaattgccgc cctgaaccgt
     4021 tccacctaaa gcaaaccccg ccccgagcac ggcgaggctc acaaccatgc cgccgattcc
     4081 cactaagaga agggggcggc gtcctgcccg gtccacgagg ataacggcgg cgacggtgga
     4141 gaggacgttc acgacaccga cgcctgcagt cgctgcgatc gaggcccccg cagagtgcag
     4201 tccggcaaac tggaagattg tcggggcgta atagataacg gtattgatgc cggtcaactg
     4261 ctgcaggatt gccaggccga gaccgacgat cagaggcatt ctcatcgacg gtgccagcag
     4321 gtcggaccag ctgccagctt tctgtgccct gaccgatcgc tcgatgtcgt cgagttcgcc
     4381 cgatacatcc ggggttccac ggatcttctg gagaacgccg gctgccctgt cttgctgctg
     4441 atgagccacg agccaccggg gactgctcgt aaggaggaac atcccgacga tgaggatgac
     4501 agccggcact gcaccgaatg cgaacatggc acgccagttg ccgcttgcgg agaagaggta
     4561 atcgaccccg taggagacga ggatcccgag ggtgatgaag agctggttga gcgagaccat
     4621 gccgccgcgg aacgctgaag gcgcaatttc cgagatatag agaggaacga cgaatgatgc
     4681 gacaccgatg gcgataccga tcagcacgcg gccgacgagg aaaacgggca ggcccgctgc
     4741 aacgacgacg acacccgtgc ccgcaaggaa gacggccgac gcagcgatga tcgagaggcg
     4801 ccgcccaatc ctgtcggcaa ggaaaccccc tgcgatcgct cccagaatcg tgccgacaag
     4861 caccgaactg gtcgcgacct cttccaggat cggagaaaga ccgaactcac cggtgatgaa
     4921 gagaatcgct cctgaaatga ccccggtgtc gtacccgaag aggatccctc cgattgccgc
     4981 tactgcagcg gtaacgtaga cgaataaggt gagaccggat ctctttggct ctccatcctg
     5041 tgctttcata ctttatccac cttcaatttt tacgcgaatt cgtcccgggg ttcttgcata
     5101 gtccgggaca cactgtcatt gtatattccc gatccgatag accaggaatg gaggatcatc
     5161 ccctggacgg agcaagagtg tctatcgagc acttccgtat ccattcgaat actcatcgtg
     5221 cgatcagcag tattttcgtc aggcttcgtt cgcgtataca taaagatagt ggtcgatcat
     5281 cctctgcaga tcgtccatcc ctgaacagcg gcggcgggaa ttacgaggcg ggttggagac
     5341 ccgcacctcg tttacccggc ccgcttgatc tcgtcgtgat cgatgagtcc gcggacgcga
     5401 acatccaccg ggtccatgtt cacgacctga ctttccggtt ttataaccca ctctgactca
     5461 atctcctctc cctgaacaaa tccttgaacc ccatcgcctt cagctgctcc ttcatcttat
     5521 ccgtcaggtt gagataccag aacaccttcc cctgccctgc actnnctctt cttctttgga
     5581 accagattca gctggtgatc atacaccttc cggagccaca gtgccatcat gttcacacag
     5641 aagatcgccc tgatccggtg gttcttccag tgacggacag gagcaatctt cagatgcgtt
     5701 ttcagcgtcc tgaaaatctt ctcgacaaaa tccttctcca tatactcatt caccacctct
     5761 gtcgccttca gagaggtgtc ggtcgaatac aggaggtact ttccatccag atcagccgcc
     5821 ctgtcaatag cttcctcgtt atactcccat gaaatcgcac accgttttcc attctgaaga
     5881 acatgaacgg tgaaaaagtc agcatactgc ccaagaatac cagcaataga ctcttcaacc
     5941 tcctccttcg ttcgaccttt acgattctcg ttaaacgcct gcaattcagc atcaagctca
     6001 gccaacatcg catttcttga atcaatagcc tccatcctct tcgcatggtt gacgtacaca
     6061 actccggcat cctccctgcc aaagagtgtt ccttttgccc tggttgcata caatgaactt
     6121 gtgccggttg atctcacccg gttggtaacc tttgttggaa catcagtttg cagaagcagg
     6181 gattgtgcct gggttgatcc tttcttcaca ccacagatca ggttccagcc aagtgcttca
     6241 atatcccgga cagactcttc cgaggtattt ccccggtccc agatcagtgt ccccggctca
     6301 taggagagat ccagcagatg taccaggagc tccttgaccg atgacatcga gttgaaatta
     6361 ccgggcagca caaagaatga aaccggcact ttatcccatt ttgagacgag aatacagaga
     6421 ttgatctgtt tctggttcgc cttcttagaa ttgtatccct tctctccaag ctcatcaccg
     6481 gagccaaaga tcagaaccgg ggtgagatcg taggcaacga tctcgtttgc agcatcaggt
     6541 aacggatgaa gtgatcgcca ttgggtatag agcgagtcac agatgagatg gctgaagtcg
     6601 gtgaacccac cagctgtaga atctttgaaa cagactctgt caagggatga atagaaggca
     6661 tggttggtga agtactcttc cggcagttcc atgaggtccg gaagagtcgt tgtctggatc
     6721 cattcaccca cgttggtagc actttcggga ttgagtgctt tattgatggc ccatgcagtg
     6781 aggattttac cgggtgatct gccttcgatc cgcccatctc cacaacagat ctgatcaata
     6841 gtcttcggca tattcagggt tgaattagca atagaccaca tgagctggat atcgcctgcc
     6901 cgttttgaat gttcaggata cagtggtgtc tcgatatggg ttgacttctt tgggagtggc
     6961 acgcctcgtt catctccttc ttttccgagg tatttgatga accgggtctt ggactttccc
     7021 tcctctcggt atgactcata gagtgcgagg tatacggcat ccttcttctt aatccgtttg
     7081 acgtgcatga aagttataat tataactcac aatatgataa agctttctgt cattattgaa
     7141 gagagatcgg gaaaatgagt tgaatgtgaa gcgggatgtt gagcgagata gagatcttga
     7201 agttagaaac tcacaaagtc aggttagata cacacgtcag ggagacgctc gtacagcggg
     7261 tgaagtctgc aagaaactat gcgcaggagc acggacggga ggcggcatgc gccgcgttca
     7321 acgacctctc cggcgaattt gccgatggcg gggcatatat ctttgcctac agtgcgaacg
     7381 ggacgacgct caccctcccc taccagccgg agctcgtcgg gacgaaccgc cttgagtttg
     7441 aggaccggta cggtgttgcg gtgaccatgc tggagagtgc cgccgcagag gccgggggag
     7501 ggttcgtata cgtcacctac tacaatcccg ataccggcct tgacgggctg aagctctgtt
     7561 acgtggctcc ggtggacaaa gagtggtttg tcggctccgg cgtatatgcc tgagagcagg
     7621 tagtaaagag gataaccccc ggccgccatc cgggcagggg ttgggcgccc ctgcaaacgg
     7681 agagatggcg ggcaatacta tttttcggcc gcttaccaga acccgggcac cagaacgaag
     7741 atcgcccagc ccatatgctc gcggctgtag ccgaaatact cttcctggag acggtggagg
     7801 tagtcgatca cgagttcgcg ctcgggatgg ccggggtttt cggcgagcca ggagaggagc
     7861 gactgccaga tccccgactc gtagcggtcc cagtcatcgt cgctggcccg gattaccgcg
     7921 gcgacgtcga gtccggcctc ccgcacgaca ccgaggatct cgtactcggt caggacttcg
     7981 ggccattcgc gagcgaactc gggcggagtg cggtcggatc cccagcagcg gtcgccgatg
     8041 acgatggtcc cctcgtccgt gaccatactg aggagagcat cgagcgtcgc cgcaaatccg
     8101 ccccagatat gggatgcccc gatcgctatc gcgcagtcga agagatcgtc ggggacgtac
     8161 gcccgggcgt ccatgcaccg gacggcgatc gcatcgccga gccccgccgt ccggaacgtc
     8221 tcctccgccc ggctgcaggc atcctcccgg gcctcgacac cgaccccgga taccccgtac
     8281 tcacggcccc agagcgcgag gatcgtgccg ttcccgcacc cggcctccac gatgcgggat
     8341 cccttccgga gccctgccgc ccgcccggcg gcgagcacct tctccggcgt ggtggggttc
     8401 atgatcgcga gcgctccctg tgatatgctg acaagttcgt tgttctccat ctatgatagc
     8461 cccccggatt gccggtctgt tctatagatg gtgcatcttt cgcccatata acccgtgtac
     8521 caccgtccgc gcttccccgt gactgccgga cagagggggc atcccggcac ccggaatcgc
     8581 tgctccgtgc cgggaagggg cgcgccgctc caccaggnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggtctc
     8701 gaagcacctc gaggtgatgc aggcggcggg tcaggtcgag gcccggctcg tcggcaacgc
     8761 taaggtctac tcggtcgcac aacgcgtacc cctccggcgt ttctctgttt tacgaagaac
     8821 ctgatcctgg tcctcgtact cgttgtcatt taactctatg aatttccttt cccgcccttc
     8881 gcatccctca cggcctgcct tctgtgtggg accatatccc gatctgcacc atggattgct
     8941 tcacgcgaaa ctacgaaatt tcagttctgt tgaatagggc atgaatgtgg aagcatcgac
     9001 ccgggatgag gaatcacccc gggcatgccc ct
//
LOCUS       DAJB01000248            9110 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_95645, whole genome shotgun sequence.
ACCESSION   DAJB01000248 DAJB01000000
VERSION     DAJB01000248.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_95645"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    2371..2453
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1970 a         2617 c         2728 g         1710 t
ORIGIN      
        1 ccggccggga gcgggttccg gttcggcttc tttctttttt ctcaggggcg ttctgacaaa
       61 tcctttcagg tcaaccatac cgatatctcc ggagaagaat ccgtgccgtc aggttgatca
      121 gaaaaacaat cccgatgaga atgaaggctg cagcgaatcc gtccgatatt gcgacaacgt
      181 tggagttctc gtaagcatgg tacactgcgt aggggagcgt gcccacgagg tcctgcaggg
      241 ggtagagttt gatgccgaat ataagatttt cgttgctctt gattgccagt tccgggaaga
      301 actgtgtgta ccccgcggtc aggatgacca cggccgattc ttccgctgcc ctgccgaacc
      361 cgaggatgat cccggttaaa atcccggaaa tcaccgtggg gatggtgacg ttccggatgg
      421 tctgccattt cgttgccccg agagcgtaac tcccttcctc cagatccgtt ggcaccgtcg
      481 cgatcgcatc ctcgatagca cgttcgataa ccgggagaat cagtatcgcg agtgcgattg
      541 aaccggagat caacgaaaag cccccggagt atgcacgcat gtaatagacc aggatcatca
      601 gaccgacgat acccaggacg atggaagggg tgccggacaa tacctcgatc aggaaccgca
      661 gcagccgcgt gagcctgttg tccggggcat accgctgcag atagatcgcc gtcccgagag
      721 caaggggggt tgcgatcaga gtcgaacaga gggagatgat gagcgttccg acaactgcat
      781 tccctatcgc gccgccgaga ccgggagatg cggattcagg ggtgatgagg aagtgcaggc
      841 tgagcgaggg gagcgcctcg atggtaacgg taccgaggat caggaagagc acgagcaccg
      901 acagcgctgc tcccccggtg cagagcagca taaagaacag ttcttctgcc tttcgcattg
      961 ttcttgttgc cactgtcatc atctcctatc ctgcttcagt cttttttcga cgaaccggat
     1021 cagcccgacg aagacgatct caaggcagaa gagcacggcg gctatcccga acagcgcgct
     1081 ccgcgcttcg tccatcgaaa cccagtaccc gatatcgttg aggatcttcg atgtcattgc
     1141 ataccccgag tccatgatgg atgaagggaa ccgggatgtg ttgccgatca gcatgaccac
     1201 ggccatcgtc tcgcccatcg cgcgcatcag cccgaggatg attccggtca gtatcccggg
     1261 agtcgcgatg ggaatgacca ccatcctgat ggtctcccac cgggtggcgc ccagtgcgaa
     1321 cgaaccctcc cggtactcgc gtggaacgga gcgcattgct tcctgtgcga gtgtggtgat
     1381 ggtcggcagg atcatgatgg caagcacgga tgcggcaagc aggatgccgg tcccgctccc
     1441 ggggttcggg tcgcggaaga tggggatgaa ccccagggtt gcatcgatga acgggtctat
     1501 gtagtagcga aaaatgtttt caagaacaaa atagccgaat atgccgtaga cgaccgaggg
     1561 tatgccgacg agcagctcga tgagcggccg tatcatcttc tccagccatg cgggagccca
     1621 ttctgcaagg aagatcgcgg tgaagaggct taaggggcag gcgatgacca tcgtcaccgc
     1681 ggtcagcacg atggtgccga cgaggaagat caggataccg tactcgccgg tagagtagtt
     1741 ccaggtcgta ccggtgagaa atccgacgcc ttctttttcg aatacagggg cggcggtgaa
     1801 aaaaatgaat ccgaagatca ggaaaacgac gagagcgctt actgctgccg aagacaggaa
     1861 gaacaatcgt gggatgtcgt atgtccggct cataaaaaaa gagttggttg gtgttccatc
     1921 ctctatgctt acgcgaactc aatgatgctg aacatgccaa catcgttgaa caggtcgacc
     1981 gcaccggggg acttggcgaa gttgatgtag tccttctcga tggcggacgg gttgccgttg
     2041 acgatgtagt tgagggggcg agcgagcgcc gagacgtagg acgtagtgtc gtcgacgccc
     2101 ttcaggctct caaggatgtg cttcttgtcg cattccttgt cgtcgacgtt cagggccttg
     2161 tgggcgctgg tgacgtagcc gaagtcgatg aagccgaggc ggacgttagt atcgctaccg
     2221 gcggcgatgg cggctgcaac acctgcgttg ccgttcacac cctctgcccc ggtgttgtcg
     2281 aagctgccac cggagtagtc gaggtattcc ttggcgacgg tatcctcggt tccggaggcc
     2341 tcagcgcgct ggtagacggt gatgtcaacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaggtac
     2461 aacgattgcc acaccggagc cgccgatctg gtaggtctgg aggctggggt acttctcaag
     2521 ttcggtatcc ttgacggcac gggaggcaga gccgatgtcg acgatgtcca tgccgacgga
     2581 ggtcacgccg gtaccagagc cgccgccctg gacggagatc ttcacgccgg gcttctcggc
     2641 catgtaggcc tcggcgagga gttcggagac cggctggaca gtggtcgaac cggcgatcag
     2701 cacctcggcc gcggaggcgc cggcgacatc gccggggttc atctgttcgg atacatccga
     2761 gctgaaggca cccatgatgg taccgacggc gaccgcaccg acgacggcga ccacgatcag
     2821 gacgagggtc gcgacgatcg gcgagaccgc gctttcattc atgttcttga aattcatttt
     2881 catttctcct ttttttcgta acacgcggcg aagcctgcaa accatttctc caggaaaatc
     2941 ttcaccatct attcggttgc cccgggtact atatatttct gacggaatag atgtagaaag
     3001 agcacaatat tatatgtatc tatatagatg caataaaaaa gccggataaa aattgccggc
     3061 gccggaggaa gcgctccggc ggcagcaccg gggtgccagg acagaacact gaagtagcgg
     3121 cgacattttc ccggaaaaat gattaaatcc tgtttttcag ccggaccgcg gcaaaataga
     3181 gggcgagggc gaccaccgca gccggtgccg ctgcgagggg gagcgcggag gccccgtacc
     3241 aggaggtcga cccgagggtg atgaacgcga gcgcggagaa tgcagcaaac accaggaagg
     3301 gccggtccgg gttctcgcgc gcgggcagga cgtttggggg tgcagaaggg cctgggggag
     3361 aatgccgtga ttcgggattg gtttgaggag acatccggac cagaggatca tatgctcccg
     3421 ggaccatata ccttcccgcc atatgctata gagatatata gaaccacggg agttcccggt
     3481 tctgcgccgc gagacctcag cgcgccgtgc cggcgcatac cggacggcgc aggacggcgc
     3541 acccgaaggc tgcgagcgcc anntccggac gatccacgac agcccgccga ggacgtgggt
     3601 tgcggcgaca agcaggatga gcgcgacgcc gaaccgatac agcccggtcg ggcggagcag
     3661 gcggagcgcc ccgaatgcca ggccgtaata gaagagcatc atggcgagca ggtagaccgc
     3721 cacgatatcg tgctccacgg cgtaccggac gagcgggctg aactcgaacc ggagaagcat
     3781 ctccctccct ccggagaaga tcagggtcat ggagtagtcg aagagcaccg caagagccgg
     3841 tatgcaggct atgatgcgat aatccgccgc cagcccggac acgccacgca gtcccgtatt
     3901 ccccatcata cctcctcctg cagaagatct ccccgcgaag agaccatgga gacaggatct
     3961 atagagccta tagaaactat agtctcacga actccagaag tatatgatat tcggcattat
     4021 ttaggtcttt cccgcagcat ggagcgatcc ggcgaaaata cgggggcggt tgtggatgtc
     4081 ccacccctat acggcagccg cgtatacaga aaccatatct ggtgcaaccg ccatactagt
     4141 gggaaatgga gtttgagtgg ggactcatca cccttccgga gaacgccgtc atcgtgctcg
     4201 aagaagcgct gggctgcacg aacgtcacct acgccctgca gctcgcatca ggcgcggccg
     4261 agcagggaaa acgcgtcacc tatatcaccc cgcattacag ggacgatgtc cgcatccaga
     4321 tggacctcta ccggatcagg cacttcgacc gcttcgcgat caccgagtgc gcgagaaccg
     4381 ccgacgccat cacgacgtcc tgcacgggag acttctgcgt catcgaccag ttcgcgtcct
     4441 tctgccccga tgccgatctc acgagactca aagcgaccct gtgcgccctc ctcgacgcca
     4501 gcaggagcgg aaagaccatc ctcctgacca tgacgaccgg catcctctcc aatcgtcacg
     4561 aacagctcat ccgctctctc gcagacggga tcatccagtt cctggaggtc aacgaaggcg
     4621 ggaagatcaa gcggcagatc aacgtcccga agatgaaagg tgagatcccg ccggaacgga
     4681 cggtgctctt ctccatcacg gaagagggga tcctcatcga cacccgtgag cgccacgggt
     4741 gaacggttca ttcccccatc gccagagcaa ccgcggtcgt ccgggcggtc gtctccccga
     4801 cggtcgcgct gacggcatag gtgccctccg ccgcgccgag gacgaccctc accgtggctt
     4861 tcccttcttc gttggtcttc accgtctcgg gctcgacgtt gccccccggg tctatcccga
     4921 acgtaaccgt cgtccgggac aggggattgc cctcgagatc ggtgactgaa acgacgatcg
     4981 ggtccttgag cggcagaccg acgagacctt cctgatcgaa cccggagatg atcgcgacgt
     5041 tcgcatcgat cacgtccccg cccgcacccg caggaaggag catcatcacg cagactatgt
     5101 agcagccccc gatcaggatc gtggcatgct tcagcccata gagcgaatcg ccctccgaga
     5161 tcttgccgat gatgaggccg ccgaagaggg cgttgagcat gatcaggtgg aagaagccct
     5221 gggtgaagtc gatgctggcg atctcggcgc cgccgaaccc cgatcccccc atatcgttga
     5281 agaaggggag gagcgaggtg ttgagcgtat agacgatgaa gacgatgatc ccctgcgcca
     5341 ggtagaagat gacgacgtac tgcttcaggt ccccttcctt ctcccgctcg atcgcgagaa
     5401 tgctccgcat atcctccgat gacttgagga tcaggtcggc caccgaaccg ccgctgtagg
     5461 atgcctggat gacaagatcg gtataccgga tgatcagttc gctcccgagg tccctcgcca
     5521 tccgtttcat cgcctcctca aaagacttcc cgaacttcat ggagttcgcc gcaacccgga
     5581 cgtcggcgtt cagcgcgccg agatccgtct tcgaaagctc catgaccgat ttgatagggt
     5641 caagcccgcc ccgcatcatc tccgagagct tgaagagaaa ctccgtgtag agctcctcct
     5701 tcttgcgccg ccgcatcttc tggagggtga tatcaaagcc gtagggcgtg atggcgatca
     5761 gcgtggcgat gatgaggata tggtcgatgc cgatcttctc cctctcttcc tgcggctcct
     5821 ctgccgcctc gtcctccgac ggcagttcta tcccctgctc ttccgcttcc atctggcgga
     5881 gaagctcctc gtaggccttc tgccggtcac tcacctgatc cgattccccg tcgcccccgc
     5941 cgatctgcgc cgccccggag agcagcatcc cggcaacgaa gagtaccgcc accgctacgg
     6001 gaatcccgac cgtgaagtac tcccgtttcg gatcgtattt gatccggata cgctcgatga
     6061 agaggccgat gccgccggga aggctgccgg atttttcgtg tctgcgtcgg agtgccatgg
     6121 tggatcaggg atttgtggag tacttgacgc cgagcatcag caggaagagg acgttgatga
     6181 gcggcgtgcc gagatagacc aggaagaaga agagctggtc gggggtgaac ggcccgagct
     6241 ccacccccag cgcgctgctg atcacgatcc cgaggacggc gagggtgagc gtcatgacga
     6301 tgccgccgag gtacccctcg ccgaagacgg agagcgactc gacgaactct ttgtccgtct
     6361 gccgccggat ccccatcaac tccttcgact tcgacgcaag atacccggca aggccgctcc
     6421 ccccgtagat gaggttcgtc aggtccatgc agatatcccg gaagacctcg gagggcgtgg
     6481 agttcaccat gctgttcatc gccgtcaccg cgtcctcgcc gaggatctcc acgcgaaaga
     6541 cgacttttct gaattcgctc gatatatgac cataatcctc acgtgcaatc ctcctgatga
     6601 tatcgagagg cgtggcaccg gaactggcca ggatggacat gaaagcaagc gcatagggga
     6661 gattcttctc gatatcgatc tttttattcg agatctggct ggtgagatag aagggaaagc
     6721 cggccgcgac gacgaccggg gcaaagaagg tgagaatgaa gatgtacagc agtgggcttt
     6781 cggaggcgaa ggggctgaaa gggacgagga atacgatgag cgagacgacg aacatgatcg
     6841 cggcgcaaag cgcagccgtg acgagcagca gcgaaaggaa caggccgggc gtcatatcga
     6901 ggtccgcctg gtagatcatc cgggctatat cctcgttcgg gtttttgtcg aggtgcttgc
     6961 cgagcacgta gcagatctgt ttgtattttt caaacagttt tgccgggtcg agagacggca
     7021 gcggaggggc tccgtccggt tttctgcttc ggaacggtaa aagcgacaat atgcgttcac
     7081 gatcgagcat gcaagacccc gatgcccctg cggagggtaa tcaacacaga atatggattg
     7141 agaggatata ggtatttcca aaagagtata tattgactat aggtttcgca cagaactatt
     7201 tatttacagg gtgaactggt cggatcgccc cccgttcgaa ggtcgattcg ttttctgggt
     7261 gcaccgcttc gagcgcgtcc cgacactccg gtccgctgct gcagacaaaa cgtactcgca
     7321 gccgtgatcc gcgggaaata ccgggcgcag tagagcaacc aggcaaccga tagaaatata
     7381 aaaaccaaga gaaaaaaata acctagtacc gcatcgccgc cttatcagga agggacggca
     7441 gatgcgagta tcagtaacac gcggtgaacc ggaaccgggc agcccaaact gcccggtttt
     7501 gacaccagcc catagatatc ttttttgtat cctgtttccg tcgcgtcgaa ctacggatca
     7561 ttctatccgc agtctcatcc catacaggcc gtcgatgaaa acgcggcagt atgttttcca
     7621 cccgcttaga ggcatttctg ctcccccgcc atatcaaacc tcaccggttc tccaggtcgc
     7681 ccagacgctg ctcgatcctc tcgacggtgc atctcatgga agcaaccgac tcctccaggt
     7741 ttgccgtgct ggtggcgatc gcctccgaca ataaccggac ccgctggagg acgtcggcag
     7801 cgaccaggat cagcgccacc ggtgcgacga cgaggaccat caaaacccac tggtaggtga
     7861 tcgctgcata caggccggcc atgatgccga gaagcatcag cacgatgagg tatttgcgct
     7921 ccatgtatac aatgacctga acgttcggag gtatatgttt tctgtcacgt tcctctacac
     7981 gcccggggac tattttggaa acggtacgtt tcgtcagtct gttccccccg gcacacgccg
     8041 tcgctcccgg agatacaggg caccaaaaga aacgatacca acgagcacga tctcctggaa
     8101 ggggggaaga acgggatacc ccagcaccag gtagcgcaga cggagaatcc cggctgctgt
     8161 gaggtcgaag acgaccgatc gtgcgaaagt gacgcggcgc tcgccgccgt cgataaagac
     8221 gatgcgtgtc aggagtatgc cgatgacgat aaggacaggg agccagagca cctggtcgat
     8281 agaagacgca aggaatgcgt agaggtaaac ccggaggatc gcgacgacaa ggacggcgtc
     8341 gaccgccttc tcgacgatgg cccttctgcg cttcgtgctt ccctctccct gttcccccga
     8401 ccggagggca tgacggagga tgctcacggg gttggggtcg accaggttct cctttcgcct
     8461 gtaccagacc atgacgaaga aatgcgccgc agtggggggg acggcggcgg cggggataaa
     8521 ggtgaccggg gggcggagga ggctaaacct cctgcagcgc ccgcataaac cgctcccgga
     8581 tctcccccgg ggcgagggga atattcggta ccgcggcatt cccgggcgcg agcacaacgt
     8641 ggataaagtt cgggccccgg ccccggtttt cccaggcctc cgcgagttcc cgctcatcct
     8701 gcaccttgca cgtattccgg atcccggcgg tgagggcgag gagttccatg tccacctgcc
     8761 cggaggccgg ggtgggctgg ttgccggtgc tcccgaaggc cccgttgtcc aggcagacga
     8821 tcgtgaggtt ctccggggct tctgccgcca ccaccgggag gacggcggtc ccgagcaggc
     8881 tcccgtcgcc gtcgaggacc acgacgtctc tatccgtcgc gagcgaaagc ccgaggccga
     8941 tcggggtcgc ctgggtgtaa ctcccgagca tgtagaagtt gaggtcccgg tcacgggccg
     9001 cgtagagctc ctttgcgggg accccgatgt tcgcgacggc cgcctcgccg tcaagggtgg
     9061 cggcgattgc ctttatcgca ttgttccggg tcatcaccgg gtcgcgaaac
//
LOCUS       DAJB01000249            8643 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_99590, whole genome shotgun sequence.
ACCESSION   DAJB01000249 DAJB01000000
VERSION     DAJB01000249.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8643)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_99590"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1845 a         2545 c         2571 g         1682 t
ORIGIN      
        1 ccccaggggg cgggggcagt catggcgata ctcggtggaa agccgtgccc ggcatcttcg
       61 atgagcaggg ctggaggcat gtgagaatag tttcacccag ccaatgcttt attttcgtga
      121 aaaaaagtaa aaactcgctc caatccttca gataaagaac cggaacgcaa tccaggcgat
      181 cagcaccatg atggccgagt acttcagccc tccaagcacc ctgccttcgc ccatggcacc
      241 tgccgcaagc cccgagaaga atccctggat cagtgcggag tggcagaaga gccggttgta
      301 gaggaagagg tccacggtgc cgatgaacgc ctggctcgaa cccgacgccg cgaccatctc
      361 gcccgcctcc gccatggtgg tgaggaaggt gctcgatagc accccgatga cgaagaggaa
      421 gacgaagaac gacatgacga cgatgaccat gtagatcatc atcccattcc tccgctccga
      481 cttcaggtta aagaactcgt aggcgtcgtg ggccgcagcc cgcagcacct cgctcacgtc
      541 gccgccggcg gaactcgcat gggcgatcag gtcgacgctc cgctcggcga gcggggtgtt
      601 caccgccttc ccgaaccggc ggatggtctc cacgaacggg acgccccagg atatctcggt
      661 atcaagcttg cggatatgcg gcgtgagcgc cccgtactcg ccctccgaaa cgatgtggat
      721 ggcgtgcggg agcgtcatgc ccgagtcgtt catcccggcc acgtcccgga agaagttcgg
      781 gagggatgcc tcgacgcttg agacacgcat tgcctccttg aggtcgaggg ctgcaagcgg
      841 cacgatggcg atgaggagcg agaagacgaa gatatcgtcg atcatcgtcg tcgtgaagag
      901 gacgctgatg ccgtaggaca tcaccaggct cataaatcca ccgatgaaga ggaggagcgc
      961 caacgggacg gagacgaaaa ggacggtagc gggctcctcc atcatcaccc ggagcgggtg
     1021 tttgatgaac cggtaaaacc cttcctgata cttccgccag ttctcaatcc caccgaagat
     1081 ctcctgctcc tgctcctgga acggatcaga cgccggattc ccctccgcca cgccgccgtc
     1141 aggagaccgg ttccggtccg gggacctgtt caaaaagtcg tcaaagattt ctttgaatcc
     1201 catatcacac ctccggggtc atggaactga tcaggatgac gaacatcatg ctcccgaacg
     1261 gcacgatcag gtagatgatg atgtagagga agaccgggtt cgagtcgccg gagataagcg
     1321 tcatgatcga ctgcaggatg atcaagaaga gcatgccggc gaccatcgcg gtgacataag
     1381 actccgcgat cagcccgagc gtctccagga acatcttctg ctgctgattg ttctcgaacg
     1441 tatactggtg ggccttggca cggaaatact ccgtaaggtt gctcccgctg gagatgctcg
     1501 caacggcccc ctgcaggaac tcccgcatcc gttcgctcgg cgtcgcctgc gagaccgtcc
     1561 gcagggcctc gaggaggtcc tttccgaaga aatcggtctc ccgggagacg taacgcgcct
     1621 cgacggcgct ttcaccgtat atcttgctct gtccgagcag tcggaagacc tcgtcggggg
     1681 tgatccctgc cgaggacatg gcggtgacgt agttgatggc gtacgggagg gtggcttcga
     1741 tatttcggcg gcgatccccg gcctgtatac cgggatagag gaggaagatc aggtacgtta
     1801 tgcccccgaa gatcaggaga gagagggccg tgatgacggc ggtgccgagc agaagtttgt
     1861 agtcgtttaa ggcgtagaaa acctcgggga ccgctccacg gtagacgatc agatcgggga
     1921 tccgcaggag gtaggtcagg agaccgataa gcacggcgga gaccagcccc acaacgatcg
     1981 cagaaacgta cgcggtcgcc agataggctt caaacggtgt attcatccgg gctcccacca
     2041 ggtcgttcct gaggctgaca aagtctgccc gcttcgcctt ataattgtgg ccgatcaggt
     2101 tgaagcagaa ccgttcatac ccattcatgc ggagccacct tccccgtaga ggtcggagcg
     2161 cacccgctcg acgaccgtct cgggatcccg gaagtaggag accaggatct tgctcacgtc
     2221 acggaactgg cggatcttct tgatgcgcat ccattcgagc acttcctgcc gccgtttcag
     2281 ttcttcacgc atccgctcct cgctccagcc ccggtcttcc atgatctgtt cgaggatgta
     2341 ggacttgccg gaatagcgga tctcgtcggt cgccggatgc caccgaaaga cctcgttcgt
     2401 gatcagctcg ttcgtccggg ggtcgatgtc caggatctcg atgagctgct tgttccgacg
     2461 gatccgctgg ccgcccaccc gggcctgcac ctggatggac atcagagaga gcgcagagag
     2521 catgttcctc ggcacgttga tcggcgggtt ctccagacgg tggacggcgc tcgccaccga
     2581 gtcggcgtgc atcgtcgcgt aggtgacgtg gccggtgctc atcgcctgga agagggtcag
     2641 ggcctctttg ccacggacct cgccgaccag gatgtactcc gggcgctgcc ggagggcggc
     2701 acgcaggagt tcgtacatat cgatctcgcc ccgcccgtcc tgcgagaacg agtcgcgggt
     2761 gatgcttgga atccagttcg gatgcgggag tttcagctcc cgggtatcct cgagggtgac
     2821 gatctttgca agcggcggga tgaacagcga taaggcgttc agcgtcgtgg tcttcccgga
     2881 cgccgtccct ccggcaaata tgcaggactt ggcgttctcg atcgcaagcc agatgaaggc
     2941 gataccgagc ggcgagaagg tgtgccactc gatgaggtct gtgggcgtga tcggctcctc
     3001 ccggaacttc cggatggtga acgtcgaacc gtgagcagtc acttccgccc cgagcgtcat
     3061 ctggacacgc gacccgtcgc tcatcgtggc gtccagcatc ggttcggcga tagagatgta
     3121 tttcccggcc cgctgagcga gtttcgtgac gaacgagtca agagccacgg aatcctggta
     3181 gacgagactc gtcttcatcg actcatacgt cgtgtgatag acgaagatgt aactgcccac
     3241 gccgtcgcag gagatatcct cgatgtattt gtcgtgcatc accgggtcga tcagcccgtc
     3301 gccaaggaac tccttctcca catggtacag aatcttctca cgcccgagcg gcgcgaggtg
     3361 gattccataa tcggcgatta ccatgttggc cgaatcgcga agggccttgc gtgcttcgtc
     3421 acgggtacgt tcacgggtgg tgacgttgag cgtctcaaag aggcgctctt tgatctccga
     3481 aaagagttcc tgctcggcag gcgagagcac gggctcgaga acattatagg tgtactcgtg
     3541 cgtcccgccg tcataggtca cccggacata ggcatacggc tcgttcaccg ggtagagttc
     3601 gatctcctcc agtcccggcg acggccgcat cgccagatcg acgataggtc catggagcgt
     3661 ggggttgtat tcctcgacca ccaccccgac tttcgggagc tgcaactttt tgaggaaccc
     3721 gaaccgggac tgcgggccct cgtctgcagg aaccgccacc tgtgcagcag ctgcggcaag
     3781 cgaagtggag gagaaagcct gggagaagag atcgtcgaac tccgatacca cccgggcatt
     3841 atcgacgaaa tcaaacgagt gctcgccgcg gttgatgctc agttcctcta ccgtgaatgt
     3901 cgcactcttc gggaggatca ggtcggccag gtttccaagt tcctcgaccg ttaccacgcc
     3961 gtcgtccacg tcttcatcag gctcgggttc atcggcctcc accacaccgg agcccttcgg
     4021 accggaggcg acggatacat cagtctcccg ccatcccctt atccggtcga ggatacctgc
     4081 aggagcagtg cgccgggaga gaggggccgg cctctccggc tccgtctcga tatctcccac
     4141 cggttccggc acggggaagg agtcctcttc gtcggcatcc tccttgtgct gcccgatccg
     4201 gtcgaggagt gccctgaccg ggtcctcgct gccggctgca cgtcggtcgg cggcatttcc
     4261 cgatctcatc actgcgtcga tcagagaacg gatctccacg ctctccggca cgggagctgg
     4321 ctccggttcc tcgtcctttt cgtcgaaaac gggcctggtg acctccagaa cctcttcttc
     4381 ccgcacctca tctccaaagg gttggaacgg cggagcggaa tcatcatgct ccgtcgtggc
     4441 ccccccgttg ccggtctccg gtttcccggg ttgtgtgaga agattcctga ccgctgctat
     4501 cagttcacgt tcttcatctt catctgctct catcctcttt gcccccaata tcaacgcacg
     4561 cttctacctg aggttcatgg aactcgatga tacgccgtgc ccgctccccg atccgatccg
     4621 agaccacgca catataacgc ccgttcaaca gcctgaaaca gactcttccc ccggaatccg
     4681 tcacatcgct tgtgatgacg acctttccct tccggatcgc gacgtgggct ccggcgaggg
     4741 gggcctgact atcgcgcacg gtcaggcaga gcacccccac ccgctgtcgg ttgggagcac
     4801 cgatggtcgg gatatcgaaa cggctgttct tcttcaggtg gctcttctcg aagactctgc
     4861 accgtgacac cagcgcgtca acaatctgcg gcgtcaccag ggagagttcg aactcttctc
     4921 ctccggtaag gtgcaggacg gccgcgtcgc cgaagagtgt ccccttctca tcgacgaaca
     4981 tggcaccatc gggttcaccc ccggagagga ccaggaaaaa cgtgtgcttc ttctctttcg
     5041 agacccctat tccagtcagg gaatgaacgg taccataacg cagcaactcg tcaagagtaa
     5101 agcgcccttc gtacgtcgac tgcacaagaa tctcgtctac aaggccatgt atgtccatgt
     5161 acatcttcag gtatggtatt ttttatgccg gaaaggggat ttttaccccc tatcacaata
     5221 tatcacatca gtatgctatt attaatacct tgtgatagcc ggatctcaat tccagcagat
     5281 attctcgtga aagcgacgta tccgtgaggc ggtagggggc cgttttcggg cagcgatctc
     5341 cagggggttt ccgccagtgg agagagccct gaccggatat tgggggaacc agggcggctc
     5401 taatcggaga gggagccata agtaccctta ttcttgcgga aaacgcatct aaacagcagg
     5461 gatcctctgc aaaaagggat cgccagaaat aagagataaa aggtggatag agtacctgcc
     5521 catgattctt ccgcccgtgg tagcggtccc gctcatgatt gcagcggctg caataggggt
     5581 cacgctcatg tacgcgtcaa ttctggacgt aaaggagcgg cgggtgcccc acagaacctg
     5641 gcgtcctgca ctcgtgatcg cgatcccgat ggccgtctgg gtgtatggac tgaccatcct
     5701 cgcagactgg caggtagcgg tggggtacct cattctggtc gcggttttct gcggattttt
     5761 ctacttcttc caggctgtaa acctctttgg gggggcggat gcgtatgccc tcatcatcat
     5821 caccgcgtgc atcccgttct tcccgatcga gccgtacttc ggcacctcac ccctcgggtt
     5881 cttcccgttc accgtgctga cgaacgccgt gctcatcaac atcgtggccc cggttgcaat
     5941 actcggaaag aacctgctcc agggtaaccg ggccccgctt ccctgcctct tcctgggctt
     6001 tcccgtcgac gggaaagcga tccagaacga atttggtttt gtgatggaag atatcgaaga
     6061 agaggaaggc aggctgaccc ggcggttcgt cggatttacc gagtcgctca ggcgcatcgt
     6121 gcggggagaa cggcggctct acacaaaaga tctcaggctg cacccaaaag actaccagag
     6181 ggagattgcc ctctaccgga aggccggcag ggtctggata tcctacggca ttccattcat
     6241 cataccgatc acagcgggat tcctgactgc actattcatg ggtgatatcc tctttgtaat
     6301 catgaaggct ctagcaggaa tgtgaaccaa tatggagata caattcaagg acggactggt
     6361 gcctgtcatc gtgcaggaga agcggactcg tgaggtcctg atgctcgcct acgcgaatgc
     6421 tacggcactg gaactcaccc ggaccaccgg gtatgcacac tattacagca ggagcaggca
     6481 gaaactctgg aagaaagggg aggagagcgg gcatttccag caggtctgcc gcatactcgt
     6541 cgactgcgac gaggacgcag tcctctatga agtggaacag accggtgctg cctgccatac
     6601 cggccatgct tcgtgcttct accggacggt cgagggggaa gagatcgccg gattagtctt
     6661 tgatccgaag aaggtatacg ctaataaggg tgaatgactt cattattatg gtgatttttt
     6721 atgaaaattg tacccgatac gagcgtcgtg atagacgggc gcataacatc gctgataaaa
     6781 accggagaat ataagggcgc aacaataatc ataccggaag ccgtcgtcgc cgaactggag
     6841 gcacaggcga atcaaggacg ggagataggg ttttccggcc tgaccgaact ccaggaactt
     6901 ttccggatgt cggaggacaa ggtcatcgaa ctccagtatt ccggaactcg cccaagcctc
     6961 gatcaggtaa aactctccag tggaggcgag atcgacgccc tgatccgaaa cgtcgccatc
     7021 gagcatggcg cccggttcat cacgagcgac ctcgtgcagg cagaggtagc gaaggccaaa
     7081 gggctcaatg tcacctactt aaggccgcag atcggcgact actcgccgct ctcgatagac
     7141 cagtacctcg atgaggagac gatcgcgatc accctcaaag agcgggctcc accggtggca
     7201 aagattggaa aactccggga cacccgcctg gtcactctcc gggatgcgcc gatgaccgag
     7261 tatgaactca agtccatggc gcaggaactc cttgagaggg caaagcgcga tccggacggc
     7321 ttcatcgagc tcgagaagcg gggcattacg gtcgtccaga tcgggtcgct ccggatcgcg
     7381 atagcccggc ggccgttcgc ggacgggatg gagatcacgg cggtgcggcc gctcgtggac
     7441 ctcgcgctcg atgattacga catggccccg gagatcaaga agcggatcct cgggaaccgc
     7501 cgtggcgtcc tgatagcggg ccccccggga gcggggaaga ccactcttgc ccagagcctt
     7561 gcgacgttcc ttgccgacca tgacttcatc gtcaagacga tggaggcacc gcgggacctc
     7621 caggtgcccg accacatcac ccagtacacc gcgctcgagg gcagcatggc gaacactgcc
     7681 gaagccctcc tgctcgttcg gcccgactac gtcgtcttcg acgagatccg gaagagcgag
     7741 gacttcaacg tctatgccga catgcgtctc gccgggatgg gcatggtggg tgtggtgcac
     7801 gcgatggagg tgcacgactg cctcaggcgg ttctgcgacc gcgtgagtta cagtatcctc
     7861 ccgcagatca tcaataccgt catctatgtc gtccagggcg cgatcacgaa gatctacgac
     7921 ctcgaactga cggtcaaggc accggacggc atgcccggcg atgcatacat ccgcccggtc
     7981 gtcatcgtcc gcgaacactc cgggcgtgag cccgagatcg agatcttccg ccacgaggga
     8041 gagaccctgg tgatgccgat catccggaag aacgctgagg agcccgcagc cgccccggcg
     8101 gcaccggtcg tcgcggcagc agcggagccc gaggagaacg tctcctggaa ggtcgccgag
     8161 aaagaggtgc agcgggagat tgggcggtac acaagcggcc cggtcgaggt ccgcatcatc
     8221 agcgacaaca aggccgtcgt ctatatcgat gataaggacg tcccggcggc gatcgggaag
     8281 ggcggaaaga acgtctcggc gatcgtgaac aaactcggca tcgggatcga catcaggccg
     8341 aggagcgaac ttgaggcgca aagacccgtc gaggaggaga tgcagcttgc cggcggcatc
     8401 aagatccgcc tcgacaaaaa gcaactgacg atcgtctctc ttgagaacag gggcaagatc
     8461 gtggatgtct ttgccggaaa ggagtatctc ttcacggcca cggtgaacga ggagggagat
     8521 atcctcctcg ccaagaacag cacgatcgcc caggagatga tcaagcgcta caatgagggc
     8581 gagactatcc ggctgcggcc ggtatgatac aggacagatg gtcaactacc cccggttgaa
     8641 acc
//
LOCUS       DAJB01000250            2650 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_130585, whole genome shotgun sequence.
ACCESSION   DAJB01000250 DAJB01000000
VERSION     DAJB01000250.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_130585"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          464 a          975 c          767 g          442 t
ORIGIN      
        1 cctgctacgc tcaactcaag tgccacgacc cgtcgggtga cgactattac gtcaccttca
       61 cccggacgac cggccggctc tcgtcctacc aggacgacgc catccggacc gcggtcgaga
      121 cctgggccga cggcgtgccc gcactggcgt gagaggggag accccccttt cactttcaac
      181 ccgcgcacgg ccttcattta tctacttctt cttctccaga ctctcctatg aaacccctcc
      241 tccgcgccga ccagaccctc tttcgagatc ccgacgtctt tgacttcgcc tacatgcccg
      301 accacctcca ccaccgcgac gcccagctcc aggaactcgc cttcctcctc cagggcgccg
      361 gcccccggaa cgccgttctc cgcgggccgc cggggaccgg gaagaccacc accgtccgcc
      421 gggccttctc cgaactcacc ggagagttca tcccggtcta cgtcaactgc cggcagaacc
      481 acacgttgct cgccgtctac cgctgcatcg caaaagacct cctcggctac acatccccct
      541 cccggcacct cgacgacacc cgggaggcga tcgccgcctg gctcggggag aaggatgcac
      601 gcctcctcgt ctgcctcgac gacgcaaact acctcatccc ggccggcacc tacaacctcc
      661 tcctctacca gctcctccgg ctctatgagg ggtgggaggt cttgggtgcc gtggtctttg
      721 cggtcacgag cgacctcgcc ctgaacctct acgcggaggc cgatgcgagc gtccggtcgg
      781 tcttccgccc ggccgaggtc accttcctgc cctacacaaa ggccgagatc cgcgatatcc
      841 tcgccgaccg cgtccggcag gggctctacc ccggggtggt gccgccgtcg gtcctcgacc
      901 ttgtcgcgga gatgaccgcc ggcgagcggg acatccgggt cgggatcgcc ctcctccggg
      961 cggctgccgt ccgggccgag aaggacggcc gctttcgcgt cacccgaaaa gacgtcctcg
     1021 acgtcgtcgc caccgtccgg tcgccggcgc ttgccgcccg gaccgctgcg ctctcttccg
     1081 gtgaacgcac cctcctctac cggctcgcgg aacagtcgct ctccgggggc gatatgatgg
     1141 caggggcggt ctttgaggag gcgcaggact accttgccat cggcaagacg acctaccatg
     1201 cccggctgcg aaggctcgcg gacgccggga tcgtcgacct cctcccgacc gggaaaggat
     1261 acgaggtctt cctccgctac gctcccggcg atgtctttgc ggcatgtgcc cacgacgacc
     1321 cggcaaaagg ctgaacaggc cgctcccctc aatcttaccg ttaattggtg atccggactt
     1381 cccgccacac tattttctca tacgataaag taacgtttat atgcagccct tcacccgatt
     1441 cagggcctgt aaggtatcct tttaatcatc ggtgtccgag gatgatgtac gcgtccggga
     1501 gcgtcccggt gcgtgaggta gcgaccatgc aacagagaag aaccagcaga ggcggcagcc
     1561 agggacaacc aaatgttcac agcaccagga tgcaccggac cgccaatggt actcggtatc
     1621 cgaacgatat aaggatgccc gtcccgggag cgtgcccgtg atgagccggg aagatacgcc
     1681 ggatgcggtc ccgctcttcc tcgtcccgcg ggcggtcgcg gacgcgatcc ggcaccacgg
     1741 gagagccgtc cgggagatca ccgtccgccg gacccgcaac caccagtatg caataaacgt
     1801 tgagcgaggg cggccatgac cgaagagtcg aaacccttcg ggtttctccc gctccggtcc
     1861 gataggaccc tcgcgcctgg aaccctctcg tacagtaccg gcggagcact cctggtctcc
     1921 gccgaggccg agcactaccg gatcgaggcc ggggaccttc ggggcctcct cttctacggc
     1981 cgggtcagcc cgatcgtcgc ggatcgtccg caggcgaccc cccgggagct tactatcgag
     2041 gggcacgcgg cgatgaacgc ctccggcaag gcagtactgc tccacacccg cgccgggtcc
     2101 tacatcatcc cgctcgtcag tttccagcag gtcgcccgtg gggaggccat ctccgcaccc
     2161 ctctttcccc tcgtaccggg gaacgacggg tgcgacggac tacgtccgga ggttgagaag
     2221 ccaacaggct tcgaaccgga gttcgagcac cagaggtgcg aggtgacagg gtgagcagcg
     2281 agatcctgag tgcggtcaat gtgctcnngt cgaggccctc gacgccgatc cggcggtcgc
     2341 cgggatctat gtcaccctca acagcgtgaa cccggccctc ctcgcccggc gggcaaaccg
     2401 gatcaagatg cgcctctccc gcaaagacgc caccacggcc gacgccgaca tcatccgccg
     2461 gcgctggctc ccggtcgaca tcgacccggt ccggccgagc ggatcgcggc ctacctcacg
     2521 gagcaggggt tcccttctcc tatcaaagcc gactccggga acggggcgca tcttctgtac
     2581 aggaccgacc tccaaaacga cgaaccctcg accgcgcttg tgaagggctg cctcgccacc
     2641 ctcgacgccc
//
LOCUS       DAJB01000251           10793 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_95550, whole genome shotgun sequence.
ACCESSION   DAJB01000251 DAJB01000000
VERSION     DAJB01000251.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_95550"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    5522..5539
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7116..7150
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1964 a         3512 c         3524 g         1738 t
ORIGIN      
        1 agtcacctgc cgccccataa accttgggga tccttcaccg gcacggtggg ggtgagggaa
       61 aaccattacg attaccgccg cgttccaatc cggagcggat gggagcgcgg tggcgggcac
      121 aaatttctgc aatccctggt atttttccac aagaccacga tgcatggacg acctccggtg
      181 acagggatgg tatcgcccgc ccgcgaccat atttttcctt ccggaggggg cctgtgcggg
      241 agagggggat accccctcac ctctgctccc tgagccgctt catgagggcg atcgcacaga
      301 agtccccgca catcgtgcat ggccctggtt cgggggcgag gttccttgcg gtttccgggt
      361 cgatggcatg ggcaatctgt ccatcccggt cgaggcggga gcgggaacgg gcgagggcga
      421 ggtcttggcc gtccgggccg tacttgatcg agtcgccgat atgcgcggcg atcttaaaag
      481 cgatgagtcc ttcccgnngc gcctgcgccg cttgcaatgc ttgcgccgac gcaccccgcg
      541 atgtggtcgt acccgacagc aatgtcagtg gggagagggc cggcgacaaa cagcggcgcg
      601 cgggagtgct cccggtaacg ccggaggtag acagggatcc tgtcggccct gacatggccg
      661 ccgagcccct cgatgatcgt ctggacgccg tgctcatggg cgtaggcggc aagccgcgcg
      721 ttccagcgca tctccccctt ctgggcctcg tccatctcat cgtggatgca cccgctccgc
      781 atcgcgttgc cgagggagag gacgatgtcg tgttccctga ggatggtgag gatctcatcg
      841 aagtgctcaa cgaagggatt ctcagcgtcg tgggcgagca tgtaggcggc ggtgatcgat
      901 ccgcccttcg agacgacccc catgatccgc tctccgtccc aggcggcgtc gatcatctcc
      961 ctgctgacgg tgtggaggac gaacgaggag accccctcat ctgcctgatg cctgatggtg
     1021 gcgaggatat cctctgcggt catagtaccg atgccgatct cggcagcaac ctggtagacc
     1081 ggcaccgtgg tgaccgggag agtggtggcg gcaaagatcg cgcgccggat cgctgtgata
     1141 tcgcccccgg tggagaggtc ggtgatggtg tccgcgccgt gcagcccggc aatcctggcc
     1201 ttctcgacct cctcttcggg atgaacccgg gacgtcgagg tcccgaggtt gacgttgacc
     1261 ttcgttcgca acccgcggcc gatgccgacc gagcgtgttt ctcgcgtcat gataacggct
     1321 tcgccgacgg cgacggagta gcggagatct tcggccttga tcccttcggc cgctgcgacc
     1381 gcttccatcc ggggggtgat ggacccctcc cgggcctctt cgatctgggt cttcatccct
     1441 ccatctgttg gattccagac ccgaaaaagt ccgcgatgga aatgctgccg ttcgatcgcc
     1501 atcccgtgcc cgataaccct gcagagcccg gcccggggag caaaccggga cgccctcggc
     1561 ctccctcacc gcacctcccc gggccggtga cgtaccttat atactatcca atcataactc
     1621 tacaggcagt gttctatctg catgagcaat ccaatggatt ataacagagg caacagcaga
     1681 aatttcggtg gaccgaggaa cttcggcggc ccccgtgaaa tgacgaagac cgtttgttca
     1741 gactgtggga aagagtgcga agtcccgttc aagccgactg agggcagacc cgtatattgc
     1801 caggactgcc tcccgaagca cagaaaaccc cggttctaaa ccaatacatt cttctttttc
     1861 aaccggagag tcacgaaacc cctgccggat ctccggtaat gctcctttca ccccgagcag
     1921 agcggcccgg aggcataacg aagagccccg ggggtatgcg gtcaccccgg ggagagcggg
     1981 ctcaccggag cccgagttcg gagaagaggg tattccggag gtcccggaga gcgtccgtcg
     2041 ccggggactc gctccgggtc acgggctggc cgttccggac cgcttcgata actatcggat
     2101 cgaacgggat ctttcctgcc accaggatct cctccttcct gcagtactcc tcgatccttt
     2161 cgcagatatc ctccgcgagg tcgaaccggt tgatcgcgac gaagatccgg acgccaaacc
     2221 gccggcagac cgtcaccagc cgctcaagat cgtggagccc ggagaccccc ggctccgtga
     2281 cgatgaggac cgcgtccata ccgccgatcg tcgcgatcag cggacacccg atccccggcg
     2341 ggccgtcggc gagcagcagg tccgcgtcgc ccgcaaggtt catggcccgc ttcttcacct
     2401 cggtgacgag cagccccgag ttcccggcgc ccgggaagag ccgggcgtgg gcgagggtgc
     2461 cccggtccgt cgccgaggtg tagatctcgc cgcagacacg gggttccatc tggaccgccc
     2521 ccgtggggca gacgtaggtg cagacagcgc agccctcaca gtgcagggcg tccaccaccc
     2581 aggcatcatc ccggcgcacg attgccgaaa accggcagtg gtccgcgcag atcccgcagt
     2641 cgcggcagag ggccgggtcg atccgggccg ccgcgagccc ccggaactcc tccgtggtga
     2701 gccgccgggg ttcgaagagg agttccaggt tcgcggcgtc cacgtcacag tcgacgagta
     2761 cctgcggcac ggcactgata tccgcgagcg cagccgccac catcgtcttc cccgtgccgc
     2821 ccttgccgct gacgaccgcg agccggatca caggctcacc cccgcgatct tcacgatctc
     2881 gccgaagaga tcggcgaacc gttcctccca cccgggaagg tcgcggcaga tgagcccgcc
     2941 ccggttctgg acggcggcga tctcccggga gaacgggatg gtcatgagga tggggagccc
     3001 gcgctcccgg cagaacccga gggttgcctc gtcctgcccg tcgctccggt tgatcacgac
     3061 gccggctccg atgccgaccc gctcagccac ctcggccgcg aggcggaggt cgtgcagccc
     3121 gaacggcgtc gactccgtga ccaggacaca gacatcgctc ccctcgaggg tctcgatcac
     3181 cgggcaggct atccccgggg aggcgtcgta gaggatgagc gggtgccctt ccgcgagcat
     3241 cttggccgcc ttgatgaccg tcggagcctg cacctctcct tcgtttaaga caccgctgat
     3301 cagcgtgagg gcgggcagcg ggcgcgagca tgccacccga ccgacggtgc gcgggacctc
     3361 ccggacggct ccctgcgggc agacgaggac gcagcctccg caggagtggc agagctccgg
     3421 gaagacgaga acgcggtctt tgaggacaga aagcgccccg aagcggcaga acttcccgca
     3481 ctcaccgcag agggtgcagc gaccgggatc gatctccggg accggggtcg tcaccggcac
     3541 gtccacggca gaagccggga aaaagagatg gagattcggc tcctcgacgt cgcagtcgac
     3601 gagcaccacc tcacgggagc gggcgagggt gtaggcgagg ttcgccgcca ccgtgctctt
     3661 cccggtgccg cctttaccgc ttgcaatcgc gatcttcatg ctgggtcccg cttcaggaga
     3721 gggaaatggg ctgcagggtt ccggcggtat actccgcgag ggcatcggcc acgctgccgc
     3781 ttccgcggta ggtgtaagcc ttgaccccgc ctgcttcgag cgcccgggcg gcgttcccgc
     3841 caacctgacc ggtgatcagc acggccgcac cgtgttccgc gagcaactgg gccgcccggg
     3901 gcccgatccc gccggcagcg cctacgaggg gattctcaac cgattccgac ttccctgtct
     3961 ccgtatcgac gaagacgaag taaggtgccc gaccgaaccg ctgctcgacc ggggcgtccg
     4021 tgcccgcggc acgtgctgtg atgcagacta tcattcagat acactcctct ggtcattatt
     4081 acccatatga gcataatact atgggtgacg caacaccgca tcactcaagc gagatggcgc
     4141 ctgccggaca ttcgtccaca caggttccgc agtccacgca gagcccgggg tcgaccttcg
     4201 ccctgtcgcc ctccatggtg attgccgccg cagggcagac cgccacacac gtctcgcacc
     4261 ctgtgcatcc ctccggatca accaccgccg gcatgctcag tgcgccccgt cgcactcccc
     4321 gccgtcttcg tgatggcagg agctctcccc cggcgagagg cggccggcga tgtagtcctg
     4381 cgcgacataa tcaacgtttc cgctgatgcc gaggagcacc tcgataccgt tctcgtggaa
     4441 gatgtcgacg gcccgcggcc ccatcccacc ggcgatgatc aggttgaccc catgccccgc
     4501 gaggaagacc gggagccgcc cgggttcgtg tccggggctc gggaggtcgt ccctccggta
     4561 gatgatcgaa tcctccacgt caaatatcgc atactcccca cagtgcccga agtgttcgga
     4621 taccatgttt ccgtcctgtg caattgcaat cctcatgatc tcacctgttc ctgcggcccc
     4681 gtgtggaccg catacaaaca tactacgcat aagcgcgaaa gtaagtaaaa ccacctatcg
     4741 taccatccgg tgccgaaccg gggaaccttt tttaatgcat gcgacatcct gcggtgtagt
     4801 tgcaggaact gtggtgaaac tgatggttca gcctttggat atcggagagt accggaacgc
     4861 ctacgagccg ggaaaggtag agtgtgccag caccggggaa atacgcccga tggaggagat
     4921 tatcggccag gaaagggcgc tccgggcgct gcgattcggc cttgagatcc gggagaaggg
     4981 gtttaatgtc tacacctcgg gcgcccaggg caccggacgg atgagggccg tccggagttt
     5041 ccttgacgag ctcgcgaagg ccaaagcccg ggcaagcgac tgggtctacg tccacaactt
     5101 cgagaaccag tacgagccca acgccatcgc cctcccggcg ggaagggggc cacggttcag
     5161 ggaggatatg aagcggttca tcgaagaggc ccgacaggcg cttccgcggg cgttcgagag
     5221 cgaggagtac gccaaacggc gcgacgagac cctccagtcg ctccagggta acagaacaga
     5281 cctcatcgcc cggatcaacc agcgcgctca ggaggcgggg tttgtcatcc agatgagccc
     5341 catcggcctc ctgaccatcc cggtcatcaa cgggaggccg gtccccgagg aggagttcgt
     5401 caccctcccc gacgacgtgc gagcggaggt ccagcggcgc cgggacgccc tgaacgccga
     5461 actccggagc acgctccggc aggtgcagga catcgaacgc cagggggccg aggcggtcaa
     5521 gnnnnnnnnn nnnnnnnnnt cgacgccgtc cagaacgata tcctcgataa cctccaggcc
     5581 ttcctcgggg tccccgagca gccgggcgcc ccgccccagt tccaggcctt catccgggag
     5641 ctccccttcc ggaagtacga tgtgaacgtc gtcgtggaca acgccgatgc cgggggggcg
     5701 ccggtgatcg tcgagcagaa ccccaccttc cagaacctcc tcgggaagat cgagaaggaa
     5761 gtccagttcg gcatcttcac gaccgatttc acgatgatcc ggccgggctc gctgcataag
     5821 gccaacggcg gctacctcat cctcagcgtc gaagacctcc tgcgcacccc cctctcctgg
     5881 gacgggctca agacggcagt gaagaccgga gaggccgtaa tcgaggagcc gggagagcgg
     5941 atgggattca tcaccgcaaa aacaatcaaa cccgagcccg tccccctcga tatcaaggtg
     6001 gccctcatcg ggaccccgat gatctaccag atcctctacc ggatggaccc cgacttcaag
     6061 gagctcttca aggtcaaggc cgacttcgac gtcgtgatgg acaggaacga cgagaacgcc
     6121 gggaagtacg ccgacttcat gtgcaacctc gtccgggagg agaacctccg gcacctggac
     6181 cgggaggcaa tcgcccgggt gatcgagtat ggctcgcgcc tcgccgcgga caaggagaaa
     6241 ctctcgaccc ggttcgccgc ggtcgctgac ctcatccggg aggcgaactt ctacgccgcg
     6301 agcgacgggg cggagcagat cggcaaacag cacgtcatga agacgatcga cgagaaggtc
     6361 taccgctcaa acctgattca ggagaagatc caggaataca tccagcggga gatcttcctc
     6421 atcgagaccg agggggagaa ggtcggccag gtgaacggcc tctcggtcat cgggctcggg
     6481 gacttcgcct tcggccggcc gtcgaaggtg accgcaagca tcggggtcgg gcgcgagggg
     6541 atcatggata tcgaacggga ggcggcgctc ggcgggccga tccacacgaa aggcgtcctg
     6601 atcatcagcg gctacctcaa caacaactac gcgagggaca agccgctcgg cctctcggcc
     6661 cggctcgtct tcgagcagag ttacgagggg atcgaaggcg actccgcgtc gagcaccgag
     6721 ctctacgccc tcctctcggc gctctcgggc ctccccttaa agcagtacct cgccgtcacg
     6781 ggctcggtga accagaaggg cgaggtccag gcgatcgggg gcgtgaacga gaagctggaa
     6841 gggttcttcg aggtctgcaa ggcaaaaggg ctcaacgggg accagggagc gctgatcccg
     6901 gcgagcaacg tccagaacct gatgctcaag gaggagatcc tcgaggccgc gaaggccggg
     6961 aagttccgga tctacccggt acggacgatc gacgagggga tcgaggtcct gacaggcgtc
     7021 ccggcgggca gccggcggga ggacgggacc tacgaggagg ggaccgtgaa ctacctggtg
     7081 gaccggcggc tccgggagat ggcggagacg ctcaannnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn cttctcggag cggttcggga acccgtcctc gctctatacc ctcgcccgtg
     7201 aggcggagga ggcggtcgag gaggcgcgag gccgcgtggc ggcggcgatc ggcgcgagcc
     7261 ccgaggaggt cttcttcacc tcgggcggga cggaggccga caactgggcg gtcaaagggg
     7321 tggcggcagc gagccggaag aagggcgacc atatcgtcac ctcctcgatc gagcaccacg
     7381 ccgtcctcca tacctgccgg gcgctcgaga agcagggcta ccgggtgacc tacctgcccg
     7441 tcgacgagtt cgggcgggtg gagccggaac gggtcgagga ggcgatcacg gataagaccg
     7501 tcctcgtctc ggtgatggcc gcgaacaacg agatcgggac ggtccagccg gtccgggcga
     7561 tcgcagcggt cgcacacgac cacggtgtcc cgttccatac cgacgcggtc caggcgatcg
     7621 gggccttccc ggtggatgtg aacgagatcg gggccgacct cctcgccatc tccgcccaca
     7681 agttcggcgg gccgaagggg gtgggggcgc tgtatgtccg gcggaaaacg cggatcggga
     7741 cgttcatgga cggtggggcg caggagcagg ggcgacgggc cgggaccgag aacgtccccg
     7801 ggatcgtggg gctcgggaag gcgatcgacc ttgcggtcgc cggaatggcc cggaacgctt
     7861 tccggaacgc cgcgatgcgg gaccggctga tccggggaat cctggagacg atccccgaca
     7921 cccggctgaa tggccacccg gtcgagcggc tggcaaacaa cgtcaacgtc gcgttccgct
     7981 acgtcgaggg ggagtcgatc ctcctcatgc tcgacgcccg cgggattgcg gcgtccacgg
     8041 ggagcgcctg cacttcggcg tcgcttgagc cctcgcacgt cctgaccgcc tgcggcctcc
     8101 cgcccgagca cgcccacggc tcgctccggc tcaccctcgg ccaccggaac accgaggagg
     8161 acgtcgacta cgtccttagc gtcctccccg gcgtgatcga gcgcctgcgg ggaatgtcgc
     8221 cactccggga gggggcctga tgaccggcat cttcaaggcc tacgacatcc gggggcgcta
     8281 cccggacgaa ctcgatgagg cgacggcgga gaggatcggg aacgccttcg tccggctcct
     8341 tgcggcggag cgggtcgtca tcgggcagga tatgcggctc tcgtccgcgt cgctttcccg
     8401 ggcgtttacg aaaggcgcga ttgcaggcgg ggcggaggtc accgacatcg ggatggcgag
     8461 caccccgctc ctcaactacg ccgtcagcga cgggaggttc gacggcgggg tgatggtgac
     8521 ggcctcccac ctcccggggg agatgaacgg gttcaaactt gcccgcgagg acgcgatccc
     8581 tctctccggc gaccgggatc tccccctcct cgagacgatg gtcggggagg aacccgtggc
     8641 ccgcgccggc gggtcctgcc gcagggccgg gatgctcgat tcctacatcg ggaaggtggc
     8701 cgggttcgtc cggaaccccc ggccgctcac ggtcgtcgtg gacgcgggga acgggatggc
     8761 cgggccggag gtgccccgcc tcttcgcgcg ggtcccggtg tggcgccttt cgccgctgta
     8821 tctcgagccc gacggacggt tcccccacca ccacgcgaac ccgctcgacc ccgaaaccac
     8881 ccgggagctc caggaccggg tcgtggcgga gggggcagat atgggggtgg cgttcgacgg
     8941 cgacggcgac cggtgcgggt tcgtcgacga gcgaggcgaa cgggtccggg aggacctggt
     9001 gaccgcgctc atcgccgcgt tcctgctccg ggagttcccg ggggcgacgg tcctctacga
     9061 cctccggtcg agccgggccg tggtggaggc gatcgagagg gccggcggcc ggcctgtccg
     9121 gtcccgggtg ggccacgcct tcatcaaggc ccggatgcgg gaggaggacg ccatattcgc
     9181 cggggaactc tccgggcact actaccggga tatggggttc actgacaacg cgctcctgac
     9241 gatggtcctg gtcgcgaacc ttctcgccgc gagcggccgg ccgctctccg aactcatccg
     9301 accgctcgac cgttatccct cgaccgggga gatcaacctc cgcgtgagcg atcccgcggc
     9361 ggtgctcgcg gtgcttgcgg cgcgctaccg ggacgcggat atcgataaac tcgacgggct
     9421 gacggcgacc taccccggct ggtggttcaa catccgccgc tcccacaccg aaccggtggt
     9481 gcggctgaac ctggaggcgg atacggcgga cctccttgat gagaagaggc gggaggtcct
     9541 ctcggtcatc cgggacgccg atccagcggc gagggaggta tgaacgccta caacaactgg
     9601 atgcagtgga aagagcagca ccagaagatg cagtaaggaa tttctcagaa cattttgcag
     9661 gcggtctctc agagtacagg cgccaaagat ccgtccgcag gttgcacggc gggcttccgg
     9721 acattaccct gtcgggtagg tggatgccct gcagaacgcg cacaatgttt ttatgatcaa
     9781 agaataataa ttatatgcat tgggattcca ggaaattcgc aataaatgcg tcgtttccga
     9841 attcaggtcg gccacatcac aattcttggg cccgtagctt agtccggtca gagcgcccgg
     9901 ctcataaccg ggcggtcgtg ggttcgaatc cctccgggcc cactcccatc ttttgcaaaa
     9961 aattgtgcag agcggatgcc ctgcatctcc gatcaccggg gcggttgacc ggcgtgggtg
    10021 tccccgtcct ccggacaccc ctcctggggc cggcggtcgc acccgacgat ccggatcgtc
    10081 ttcccgtgca ccagggcgtc ggcgaccttc ccccgcgcct catggaggtc gcgccagagg
    10141 gtctttcggg agacgccgag ggcgacggcg gcctcttcct gatcgagtcc ctggaggtcg
    10201 acgagccgca gggtctccac ctcctcgggg aggatgacga cgaactcgtc gggcctcccg
    10261 cagagggggc cgaaacaacg aaacactccc tgatccccga tagtccggcg gacccggggc
    10321 cggccccgcc cgcggtggcc gtacccctcg ccgggctctc tctcgttcac ggtatcatct
    10381 ctggagaaga actccgtccc acatgcagat agtgccttcg cttcggcacg gtaccagccg
    10441 ttaccaccgg ccgcgtgccc ggccgccgct accgagaccg cgaccgcatc cccatgggat
    10501 accgccccgg ccgatgccgc gaagcaccgg cgtggcggac tcgccttccg ccgccgcagt
    10561 cgggggcaga acgcaccggc cccgccggcc gcccgtcatc gggccacggc ctgagggtcc
    10621 ggttccatca aatcctggca tggtgtatca cctcaattag tacacatatg ggtaaaaata
    10681 tattaaagta tcttatcggg cagaacaggg tagggccggg gccagggagc tgttttcgga
    10741 tgcaaagaga ggggataggc cccccgtgga gaagagggtc agccgcgctt ccg
//
LOCUS       DAJB01000252            9979 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_117641, whole genome shotgun sequence.
ACCESSION   DAJB01000252 DAJB01000000
VERSION     DAJB01000252.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_117641"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1995 a         3110 c         2802 g         2070 t
ORIGIN      
        1 ttgaaaccgg gggtctcctt gcccgtcgtc tatgaaagac ctggtcgact actggcagca
       61 ccggtacgac tggcgcaggc acgaggccgc actcaaccgc ttcgctcact tcagggcaga
      121 gatcgacggg ggggatccac ttcatccacg agcgcgggag ggagccggac ccgacgccgc
      181 tcctcctgct ccacggctgg cccgactcct tctaccgcta ccacagggtc atcccgatgc
      241 tcgccgattc gttcgatgtg gtcgtcccgt ccatccccgg acaggggttc tccgaccgaa
      301 agcccatgac caccgacgac accgccgatc tcttcgccgg actgatgacg gaggaactgg
      361 gctacgagag attcgtcgcg gccggcggcg acgccgggac cctgatcgct cagtcactcg
      421 ccgaacgcca cgccgacgcc ctgctcggca tccacctgac cgatgtcggc tacccggacc
      481 agacgacgga tttttctgcc cttacaaagc ccgagatcga atttgcgaac tacattcaac
      541 agtggtggat gaacgaaggc gcctttaaca tggtccagtc gaccaaaccg cagagcctcg
      601 cctacgggct ggccgattcg ccggcaggcc tcgcggcctg gatcatgagt ttcatggcct
      661 cgggcacgcc ggcgaggagc tcgatacgcg cttgacccgc gacgaactgc tcacgaacat
      721 cacgatctac tgggtcacgc agacggtcgg cccgtccctt cgcaggtact acctcgacgc
      781 tcacgctcct ccccgtccct ggcagcgcac cccggttccg gcagccgtcg cccaccctcc
      841 ccgggacgct ccgctgcccc gcgagtgggc cgagcgcagg gtgaaccttg agcacttcac
      901 aaatctccct cgcggcggac acttctcggc ctgggaggag cctctcctct acgcagggga
      961 cgtgcgggag ttcgtcgggg aactgcgcaa tccttaggca tccctgccgg gcggctgtgt
     1021 cgcaccccgc gttcctggcc tgattacttc cggttttcac gccgatatgc ggcaaccgcg
     1081 ggaaaacgga tggtcaacca tatatggctt cggcattaaa cgtatgaatg tgaaatcggc
     1141 ggtactggga ggtggtctca ccgggattac gctggcccgc ctgctccatg aacagggcga
     1201 cgacgtgact gttcttgaac gggacgagac gatcggggga ctctgccgct cgagaactga
     1261 ggcaggtttc acgttcgacg tcgggggctc gcacatcctc ttctcccgcg ataccgaggt
     1321 cctctccttc atgctctcgg tcctcggcaa gaacgccgac cggcggaagc ggaatacgaa
     1381 gatcctctat gcgggccgct acgtcaaata ccccttcgag aacggcctct accagttgcc
     1441 cgacgaagac cggttcttct gcatcaacga gttcgtcaag aacctcatcg ccgtcgagaa
     1501 gggcgaggtc ccgccgccgg aaaactttgc ggagtggatc acctacacct tcggccgggg
     1561 gatcgccgaa tgttacatga tcccctacaa cgagaagatc tggaactttc cggccgaccg
     1621 gatgtcgcac cactgggtgg aggggcgtat cccccggccg ccggtcgagg atatcatccg
     1681 gtcggccatc gggatcgaga ccgagggcta cgtccaccag gcggtcttct cctacccggt
     1741 gacgggcgga atagaggcgc tggtccgcgc gatagccgag ccggtcctct ccgccatccg
     1801 gaccgggttc tccgtcgcgt ccgtccgcga ggagaagggc gggtttgcgg tgagcgacgg
     1861 catcaagacc gtccacgcag accgcctgat atcgaccatc ccgctccaga acctgctccc
     1921 ctgcctcgca gatgtcccgg ccgacgtgca ggcggcgtgc gacgctctcc ggtacaactc
     1981 gctctgttcg gtcttcatcg gcctatcggg cgaagttccc gatatctcgt ggctctacgt
     2041 ccctgacccg gagaccggcc tcttcaaccg gatatcgttc ccctcgaact acagcacgga
     2101 agtcgcgccc ccgggtcact cctcggtcct cgccgagatc acctacaacg aaggcgacgc
     2161 ggtctcggag atgacggacg ccgagatcat cgagcacact atcggttctc tcatcgccgc
     2221 ggggatcatc ccttcacggg ataacgtcgc ctacaccggc gtcgcacggc agaagttcgc
     2281 ttatgtcgtc tacgacaccg attacctgca gaacatcgag atcgtccggg agttctgccg
     2341 ggagcgccgc atcgatctcg tcgggcggtt ttcgcagttc gagtacctca atatggatgg
     2401 gtgcatcagg agcgcgatcg acttcgtgag ggcgaacgga tgaaggtcaa catcttcgtc
     2461 gaggatttcc gcttcctgcg ctacatcggg tgcgcaacgg cagcgaggac gctctataac
     2521 cacctcgccc tgctccccga tatggaggtc tcccgcaacc tgtatcacgg cgacttcgac
     2581 ctgacccact accacacctt cgggccctgg gcgatgtatc acctgaagtt caccgagggc
     2641 gcaaaggtcc tgaccgccca ctcgaccccc cggttaaacg aggggaacgt cgccttctca
     2701 aagcggataa acacggtata cccgaagatc taccggcagt tcgaccatat catcacgata
     2761 tcggagccct gccaccggga gaccgaccag ctcgtccccg atgtcccaaa gaccctcatc
     2821 ccgaacggca tcgaccggaa gtatttcaaa cgggacaaga agaagaggaa gtcgttccgc
     2881 gacgagtacg ggatcgatcc cggccggcga gtggtcctct ccgtcggtca gcagaccccc
     2941 cggaagggca tctacgactt cttcaaactc gcggaccagc accccgagat gacctggatc
     3001 tgggtgggcg ggttcccgta cgggacgttc tcgaaggatt acgcaaagat ccagatgctc
     3061 aagacccatt gccacgagaa cgtcatcttc accggcgtca tcgacgacat ctcccgggtc
     3121 tacagcgggg cggacatctt cttcatgcct tcccacgccg agacgttcgg cctcgtgatc
     3181 gtcgaggccc tctcggcagg cctcccggtt atcgcccggg acatctatga gttccgggag
     3241 atcttcgggg actcgatcct cttcttccgc gagaccgacg aggcacggga cctcttcgcc
     3301 gacgacgcag ccctgcagcg ctgcgccgcc cgcgcccgcg cttcaacgga gaagtacgac
     3361 atcacgctta tagcaaaacg gcatcaacaa ctataccggg agttgatcga gagatgatct
     3421 ccgtagtcgt tccgacctac aacgaagagc agaacatcga gggctgcctg cggtctctcg
     3481 ccgaccagac ggtgccgagg gatacctacg agatcatcgt cgtcgacgga aactcaaaag
     3541 acaagacccg ggaactggcc gaacctcttg cggacagggt ctttacccag acgagcaagc
     3601 gagtcggcgg ggcccggaac gacggtgcga tggccgcaaa aggcgatatc atcgcgacga
     3661 ccgacgccga ctgcatcctc ccccgggact gggtggagcg gatcgagaga aattttgcag
     3721 acagagcaat cgtgcagctc tacggcacgg tctacccgct cgaaggcggg atcaggaacc
     3781 atctctcgct tgccggcgca aacaccttcg cgcgcctcgg ctattatacc aggacgatct
     3841 actttacgct cgggtgcaac acggcgtttg accgcgaggc gttcctccgg gcggggatgt
     3901 accgctgcat cgatgccggg gacgacctcg agatcgcgca gcggatgcgc aagatcggca
     3961 aggtctacct cgatcccggc ctgaagatcg ggttctcgat gcggcgctac cagcagttcg
     4021 gcacgctcaa gtcgatatcg gaatggctgt atatcgttct ccgcggtgga gaagcaggcg
     4081 gcgctcacta ctcgcagcgg gagtacaagt aggcgtgaca gacgagaggc cgatcccgga
     4141 ggtttacacc gaagcggtgc gccggtccga gacctgcggg tttgcacgcc tgctcgggat
     4201 ggtggtcacg gagatcgagg ggggctgggt cagggtcgcg atgccgattg cgggcaagga
     4261 gaacgtccac ggcacgaccc acgggggcgc gatcttcgcc gtcgcggacc acgccttcgg
     4321 cgtcgccgca aacctggacg ggatcgacca gatagcgatc tccgcgtata ttcagtactt
     4381 ctccgtgccg gcggcgggca cgctcgaggc gatggcctac cgggtctcgg agacggagaa
     4441 gacgtcggtc tacgcagtag acgtctactc gggcgaccgg cgggtcgcaa cgttcgaggg
     4501 cgtcgggttc aagatcaggg tttctgcgaa gaaagagtga agagggctgt ttttgtgccg
     4561 ggcgatgttc cggctttcgt tcttgtgaca cggccagttg tcacttgcat acattggaat
     4621 ggcatatcgg gagaaaattc gtgacactgg accgtggaga cgacgcacct aacggtgctc
     4681 acgctcctgc ctcctggcct gtcacgtaac gaaccacgga tttcatgggg atatcgccac
     4741 gcactgcccc cgcccctgag gggcggggga ggaggccgaa ggccgggcgg ggtgggggga
     4801 aaccattaga ttggtttaac aatgagcgaa agattcagga acttgggtac aagactccca
     4861 atgtgtactg aagaagaaaa aatcagagtt tgcgaggggt taacttttta acatcgttgg
     4921 tttctcaatt atgcaataca ccttctcata gcaaacaact ttctgaatcc tatgtccacc
     4981 aatgtgagcg agaggttttt caactatctt agcggcctga caagccaccc tatcaccttt
     5041 caaaccatca ttgagtgcat ataaaattgt catgctgctt caacaaccct cccctcttca
     5101 attagttcgt tgattatttg tacaaccttc tccaaatcaa aattaaattc acaggcaata
     5161 tctgaagggt atacaacctc atgtttggag taataatcaa gaatgacctt tctcatttga
     5221 tcgtaactta cttctacgaa atcgtctgag agagagcaca ttgccccctc atctcctctc
     5281 cctagcaata aaagtatctc gcggttttgc tcttcaatgg aattaagttg gatctcaagt
     5341 gagttaagtt ttgtatatat ggtttggtaa cagtcaataa taaaacggac gttctcatgt
     5401 aattgtttta acctggcgtc aaactcagtt tctttatggt atatcacccg atttagataa
     5461 taaggattat tcgatggatc tgttgcagca taatattcac cagtgagata gtttttatca
     5521 ggccattctg ccgatacctt tctttgggat tgtgtaactg ctgccatatt tatcaagaaa
     5581 ggaggtcttt catcatggca ccgatctgcg ggggtatctg aggtacttta ctaggtttgg
     5641 atttacctat tctggaccac tttaattgga attgagtggc actgtacctt aattttatca
     5701 atgaattaag aatatccatt ttatcgtttg cttcaacgga tgcaactaat tcattatatg
     5761 cagtttgcag cctctccatg gtatccgtaa gctcttcatc agggatttga taagtatcaa
     5821 ttacaggtcc tacttgagag aacactccct caagaacttc actaataaaa acaccctctg
     5881 ggtattccat aagataacca aaaagcgtaa gaccacttga tgttttgaag gcactttcaa
     5941 tatcttgttg gcctaaaaga acacctatct ctcttaagct atcgagaaac acgacactag
     6001 aatccttcat aaatatcaaa caccttacta cttatttagt ctctcacttc ttccccttgg
     6061 aagataaatc ttttgaattg gctttaacgc tattttcgtc aatttttacg cacgagaaga
     6121 ggtacgctgt cacgttacac cacagataag gtgggacacg ccctctcaaa actcttcgtg
     6181 tttctcatgc tcgcttcgct cgcactcccc tttgtcccca ccccctatgg cgatatcccc
     6241 gcagtccact gcaccaggct ttttcctcgt ttcagtcgct ctgtctgccg caacttactc
     6301 gcaccttcag tgctcatgtt cctgcccttc ggcaagtcac aattcacacc tacaagtgct
     6361 catgctccag ggatttacag tctcacccaa ccaaaaccgg attccccaac ccccctcact
     6421 tcggcttata ccgcccccac ttctcaagcg ctcccctcag ttcgggggcc ttcttcgcct
     6481 tctcctcaag cgcctccccc tctttccagg cctcgatcgc ctgcatggtg gcctctgcgc
     6541 ccgcccgcgt gcccttcggg tggccgtgga tgccgccgct gaccaggaga acgagttcgg
     6601 tgccgtagat gtcgagcacg tccggcacga gcccggggtg gagcccgccc gacgaggcgg
     6661 gaaaagcact cttgatctcg ccccagtcct ggtcgagggc catgtggtcc accgcgttcg
     6721 tatgcttctc ccggagcata tccgcaagca cggtggcctc cgcccgcgtg ccgaccagtt
     6781 tcccgacggc cgtgccggtg tggatctgcg agacgccgat gagccgcatc atcttcgcca
     6841 ggaactgcat cgtgatcccg tgcctctcgt cccggtcgaa ggccgcgtgc atcgcccggt
     6901 gggcgtggat cgcaagcccg aggtcggagc agtagtcccg gagggtcgcg accgcggcgg
     6961 tcccggcaac cacgacgtcg atcatcgcgt agttccagcc gtagtccgca agcagcttcg
     7021 cccgcttctt catggtctcc gtctcggccg tgatgttgat gaacgcagac ttcacgtcgc
     7081 cggtctcctg ctcggcctta tcacgcatct tcgtcatggc ccggacgcgg tcgtcgaacc
     7141 ggttaaacga gtgggacgtc aaattctcgt cgtctttgac gaagtcaaac ccgcccatcc
     7201 aggtttcgta gccgacctct gcgtgctcct ctgccgtgaa ccccaccttc ggctttgcga
     7261 ccgcgcccgt cagcggcctg ccgtggacct tcatcatatc ccggatccct tccatgccga
     7321 agtgcggccc cttgaagtgc cggaggtatt cggccgggag cgaggcatcg atcagccgaa
     7381 ggttcgcgag cgccttcatc ccaaagatgt tgccggcgat gccgctcaag agctgggcag
     7441 cgttcccttc ctcccagaga gcgaggggat aggcgatctt cacgtagttc ccctcgatct
     7501 cgaacgcctt cgcctggagg tcccgcatcc ggggcggcag ggtaaagagc gtggtccacg
     7561 tcccggtcga actctcggac gcgattctcc cgaccgcctc caccctgctg atcccctccg
     7621 cgggctcgaa ataatagagg gccacgagtt cgtccgggcc gggggtatgg tttaagtcaa
     7681 caaactcttc gtaccagtca atcgccatag aatcacctct tgaagaaagg ggtgcccggg
     7741 tagaaaaacc ttccattcgg agcagggcag agaggcctgc aggcgccgga tttatcagcc
     7801 ggcggtccag aaggtgcatc atggaaagac ccgttgagaa cacccgctcg ctcttcgact
     7861 acgacgcagg ggagccgccc ggtgtcaggg cgaccggcat cgatgtgacg atccgcgacc
     7921 tgacgtatca ggccgctcca gaacgctggg tccgggccta tctggtggcc ccgacccccg
     7981 atcnntgggt gcggtctccc tgctcgtcga cgccccctgg gccgatggcg gggcagcggc
     8041 ctggggccgg gcggttacgg accccgggga agcggtgtgg gagcacgtcc ggacggtgat
     8101 cgaccttcgc cgggggcttg acctccttat agcgcagccg ggcgtcgacc cggaacggat
     8161 cgggttcgtg ggccacagtt tcggggcgct catcggagcg gtgctcgccg gcgtcgaccg
     8221 gcgggtgaag gccgcggtcc tgatggcggg caccgggagg tttgccgacg tcgccgccgt
     8281 gaacctgccc gatctgcagg gcgagaggct ggagcactac cgccggacgc tcgcggagat
     8341 cggccgggcc gcaccccccc ctctcctctt ccagtccgct cttcgcgacg aggtcttttc
     8401 ggaggagcag tcccgggaat tcttcgagtg ggcgaacggc ccgaagtcgc tcgaatggta
     8461 tgacgcaggc cattacctgg atgaggcggc ccgccgcgac cgtatcgcgt ggctggccgg
     8521 gcggctctct ctggagcggc gggagccgga ccgttcccgg tagcggttct ccgggatttg
     8581 aacccgtgcc ttcccgggta agggtgcgat gacggcagga acgggatcca aaatattaca
     8641 aacagtgccg atgagtaata attggtaaaa ttattatagg gttaattcat actagtgtgt
     8701 ggccaggcat tgcccgtgat gaaccctgcc tgcgcctcgc ctctatccgt cataccattc
     8761 cgatgcgcta ctgcacgtcc ttccataccc acccaacaac tttttgccgt ccgtcccggg
     8821 cccgcaccat tcctgccgga gtgctcccgt tgcccttccg cggggggtaa cgcatcactc
     8881 ggcaatcccg tcgagggcct tctcgatccg gtccaccttc gcctcgatcc gctccatcga
     8941 ctgccgcagc atctgcagat ctgcaccggc caccgcgttc ttctgccccg ccgcattcgc
     9001 tatctggtct tcaatccact ccttgagcct tacgacaatg atgaagagca gcgtgaggat
     9061 accaaattgg atgataatcc agactgaccc ggcgagcacg gcattcaagg agtgcgaacc
     9121 gccgagcaag ccggcaacaa ggctgctgat cacgagcagt gcgatgacgg cgccccagag
     9181 caccttcagg tagagtttcc acctatcctg tttcatcctt cgacctctct aatgacataa
     9241 tcacgtccag gttcagcacc agctcaaacg gcaccgcccg gtagtacctc cgcaggtacg
     9301 gcggctcgtc cgacgtcctg ctggtccctt ccgcgagacc gagcttctcg agctgccgca
     9361 ggtagatggc gaccagcggc cgcgatatgc cgagctcttc ggcgatctcg cgggcatact
     9421 tctcgtcggc cgcaatcgcc ctgagtatgg caagccgctg ctcgttcccg agcagggaga
     9481 gcagccgtgc cagttcgggg agggtgctga aagagaatgt caacaggtgt taattatttt
     9541 ttacacatat gaatattccg ataccacatc tcatacgccg cccgggcttt ccgaaccgcg
     9601 aattgccctt tttcggtgga taggctggac tcgctccccc tcctttcccg gctgtcgtac
     9661 cgtaaccttc ttgttccgcc gggtgcgttc acgggctgca ggagtctgag tatgaccgct
     9721 gacagggtgc ggaagaacac gccgcccgag attaacgccc ggatcgatcg cgagatcgcc
     9781 gggagtattc gttattttgc cgcacaggac aacttcgaga taacgaggcg tatccgtgaa
     9841 ccagaccgtg aatgggatgt cgagcgggcg ctggaggcga atgccgctct cctcggggcg
     9901 ataggactcg cgctcggaat tttccgcgac cggcggtggc tggtcctgcc gttcctgctg
     9961 cagcacgccg tgcagggat
//
LOCUS       DAJB01000253            2890 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_131495, whole genome shotgun sequence.
ACCESSION   DAJB01000253 DAJB01000000
VERSION     DAJB01000253.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_131495"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          634 a          811 c          862 g          583 t
ORIGIN      
        1 tgtcggacaa tcgcgatctg ttcacatcca gtcatgcagg catggggggt tcaaggttca
       61 agagggtatc gccaggaact ataatcgaga tcacaggcct gtccggcctt gccgtgaacg
      121 gcaccgccac gatcatgatc ccggttccgg cgaacgcgga gggcgagctg gtgatcttcg
      181 aggcgtccag cgttcgccag cccgccgggt ggcggacggc gatccgggag acagagtacg
      241 ggaagatgct cgcgttcacg acgacggaag actacgcgca ggatatcttc gtaccgaccg
      301 gtgagttcga gaagagagaa gagccgcggc tgctcttccc cgtgcttgcg atgcccgaca
      361 acgcgagcgt cgccgagttc acccggggat cgggcgggac ctacaccacg gtcgtcttcc
      421 tcgacggctt cgtcccgccg gagaacacca catcgatctc gttcgacctc agataccggg
      481 gcggcggggg catgaaatac ctgatcaaag agaatatctg gacggcgacg gtgaatacgg
      541 ctgttccggg cacaacgtcg ggatttgttc cggtgccggc cgactactat gtcactgccg
      601 gggggatcta tctttgatgg acgattccgt gcgaaaagtc cccggcggac cgtgagcgtt
      661 gcgccccccc gaccatgtgc tcttccagtc ttgcggatta gaaggtgtca ggaagtgaat
      721 agaagatggt tccctcgata atctcctttt taggcaaagt tatcggtgtc tcgcttcttg
      781 tgatcgcagt tgttgctatc ggcggaaaca ttctcgtcta ctattatcca acggtgccgg
      841 ggcattactc atacgtcgtt tccattaccg gattgtccga ctaccaggaa gatcccatca
      901 cggagatagt tgttcccgtc cctgcgatca gcggctcacc ggtcttttcc gaaaaggacc
      961 tacagcatat ggtcaccgag aactgtacac ccctgcctgt tatgacagag gatggagaaa
     1021 tgctcgcatt gcgcttggtc ggtacagatc tcacagacat ctctgccgct atctcacggg
     1081 atttctcaaa agatccctcg cttgaggagg tgcaggaagg tgtgtttgta ccgagatcct
     1141 cacggccctc tgaggcgggg agcaccccgg atgacttccc atacattatc atcccggatt
     1201 cgctttgccc cgtatcgagt gatccgaacc ccatctcggt aagtatcaac gttagcgtat
     1261 ccggcagcac caccttcggt gagcacaggc ccgattacct tgtctcgatc gtggagcaga
     1321 tcccgcccgg aaggaccggt attatccccg tggagccgcg gatctactat cgggatagtt
     1381 ttcgtgaagc cttccgtcct ctggaggaga acccatcaca acctttatag ttaggggcaa
     1441 cctaattaac caggtatcaa ctatgccggc gagacacgag cacctgttcg aagtgtttga
     1501 tcgtctgatc gccatcaaga acgaatgctc ctctgagatc ttctccgaat gcgggcttgc
     1561 cgacgtgacg gtgaagcagg ttgcctacct gaagacgatc gacgagcacg gcgacgtgac
     1621 gttcagcagt cttgccgaga tcaccaggaa ctcgaaaccc accgtgacgg agatggtcga
     1681 tcggttcgtc cggatggagt gcgcctaccg ccagaagtcc cccgatgacg ggagggtcac
     1741 ctacatccgg ctgaccgaga agggccggat gctggcaaac gccgagcgga acgcgctgaa
     1801 ccggatgatc gagaggatgg tgcggacgct tgatgagaac gaagtggatc tcctggtgga
     1861 gatcttaaag aaggtcggat gatttttttg gccggcatta acgttaggta aactcgaact
     1921 aaatgattgg agtcaccatg cactcaccac aacctgcaat gatccgagag aagatcgtca
     1981 taccgctctt cgagaccgtg gtctacccgg agagccggac gaagttcccg gtcgacacgg
     2041 ccaccgggga ggcgctgctc tccgctatga agaacgacgg gtccgcgtct gcggtcgggc
     2101 tgaccgtaaa gtgcggcgta cagccctcag aggtatcggc cgaagcgttc tatacgaccg
     2161 gaaacctcct tgagatcgcg cacgtacagc ctgccgacga cggctacctg gtctttgcgc
     2221 gggcagcctt ccgggttaag gctgtaaccc tctccgagaa tgacgggcag ttctctgccg
     2281 tctacgaacc gcttccggat ctgccggatc tcgacgaggg gacccgggct aagatgcttg
     2341 tggatatcag aaaggttgtt cacgagatca gcggcaactt ccagggctcc gaacagttca
     2401 cccgccccgt tgacaggatg gactccgtcg accagatcat agggttcgtc gtgccgttca
     2461 tgccggttga tgttgcagtg aagcagaatc tcctggagac cgtctctgtt cgggagcggt
     2521 acgctgcgtt ccttgaagtc ctcacgaacc tgaaagagag catcgatctc cggatcgagg
     2581 tggccaggaa ggtctccgac aaggtaggca aggcgaaccg cgaagcgatg ctccgggagc
     2641 agctcaaggt gatccaggac gagctcaacg gctgcgacgg ctcatccggc gaggaaggtt
     2701 accgggagcg gatcgagcgc tcgacgatgc ctgatgaggt gcggaagaag gcgttttccg
     2761 agctcaagaa actcgagagc ggcgggagcc agcaccacga gagccagggg ataagaaact
     2821 acctcgacct cctgctcgac ctcccctgga cggtcgagga gaagaagagc atcgatatcg
     2881 cagaagcccg
//
LOCUS       DAJB01000254            6307 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_104948, whole genome shotgun sequence.
ACCESSION   DAJB01000254 DAJB01000000
VERSION     DAJB01000254.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_104948"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1380 a         1902 c         1881 g         1144 t
ORIGIN      
        1 cactctaatt gttccgccta aaacaggatc gaccggttat ttcgcaacga tcccggcacc
       61 gggaaatcat ctcttgttag aatactattt attaaccgga aaaacaatat agctgatgaa
      121 gtaaatattt acaactatat atgcgcctgc tgtcgggcct gacagcagag gagagagtaa
      181 tgccgcccga caacgccgat attacccatg cacctgccca aaagacgcat ccggaagtgc
      241 agctggatgc gcctcagtac tacctcaacc gagaactgag ctggatacag ttcaaccggc
      301 gcgtcctcga ggaggcgcag gaccaccggc acccgctgct ggagcgagtg aagttcctct
      361 cgatcttctg gagcaacctc gacgaattca tgatggtccg ggtctccggg ctccggcggc
      421 agcggacccg cggcgcactc gaagcccccc cggacaggat gacgccgacc gagcagctga
      481 cagcgatccg tcaggacctg acaccgctgc ttgaggaggc ctcccggtgc tggagcgaag
      541 acatcctccc cggactgaac cgggcgggca tcctcatcca ccggcaccag gaccttggtc
      601 ccgaccagaa acgggccctg gaagacttct tcgagcagga ggtctttccc gtgctcaccc
      661 cactggcatt cgacgtcggc cacccgtttc cgttcgtctc aggcctgagc ctgaacctcg
      721 ccgtcatcat caacgatccc gagcacggaa cggtcttctc ccggctgaaa gtgccgctcg
      781 acatcctcca gaggctcatc cgggtcccgg gcgatatcct cgacgccgat gacccggaga
      841 gcctttccgg ggcgaacgct caccacttca tcttcctcga ggatctcgtc gccgcgaacc
      901 tcgaccggct gtaccccggg gtagaggtcg ttgccgcata cccgttccgg atcacccggg
      961 atgcagactt cgagatcgag gaggacgagg cctcggatct cctcaccgcg atcgagggga
     1021 gcgtggagct ccggcggatc ggggaaccga tccgcctcga agccgactgc tccatgccga
     1081 catgggtgag agagatgctt acggcaaaat tctgcctccc ccagagcctg atttttacgc
     1141 ccgagggccc cctcggcatg gccgacatca tggaacttgc agcggtcgac cgcccggacc
     1201 tgaaagacca gccgttcctt ccctcttacc cgcccgccct ctccggcgaa gaggatatct
     1261 tctccacgat ccgaaagcag gacatcctgc tttaccatcc ctatgacagt ttttcacccg
     1321 tgatcgagtt cgtcaaacag gctgcatgcg atccgaacgt gctcgccatc aagcagaccc
     1381 tgtaccgggt cgggttgaac tccccgatcg tcgaagcact gatggaggcc cgaatgaacg
     1441 ggaaacaggt tgcggtgctc gtcgaactga aggcgcggtt cgacgaagaa aacaacattg
     1501 gctgggcgcg ggcgctcgaa cgggcaggtg tccacgtcgt gtacgggctc gtcggcctga
     1561 aggtccacgc aaagatgtgc atggtcgtcc ggcgggaggc aggagcgctc gtaacctata
     1621 ctcacatggg gacgggaaac tacaacgccg ggacggcgcg gatctacacc gatctcggcc
     1681 tctttacctg caacgccgag atcggcgcgg acgtcgccga cctcttcaac gcgctcaccg
     1741 ggtgctccag gaagtcgggg taccgaaggc tcatcgtctc ccacgggtcg atggggacca
     1801 tgaggcagga gatcatcgca cggatcgagc gggaggtccg gcgccagcag gagttcggtg
     1861 acggctatat cgccatgaaa ctgaacgcac tcgtagacca cgactgcatc atggcgctct
     1921 accgggcgtc cgaagccggt gtcggagtgg acctgcaggt ccgggggatc tgctgcctcc
     1981 ggcccgggat acccggggtc agcgatacga tcaccgtcac ctcgctcgtc gggcggttcc
     2041 ttgaacactc ccggatctac tacttcagaa acggcagaaa cgaggaggtt ctgctcggga
     2101 gcgcggatct aatgccgcga aacctcgatc gacgggtgga aacgctcttt ccggtcgtcg
     2161 atccggagat gcgggaggcg attgtcaagg atatcctgca ggtccatctc caggataccg
     2221 ccatcgcgcg aatgttacat tgcgacggca cctacgagcg gatacgcccg gctccgggag
     2281 aagagcctct caactcgcag gactggatgc ttgcacaccg tggggggtgg catgatgacg
     2341 agtgcacatg atcacgggac ctgtctgtac ggggccaggt acatctccgg ccttctcaaa
     2401 gccttcagcg ccgagatcga aggtgtccgc gcagcagacg atatcgaatc tgtccacagg
     2461 atgcgggtgg catcccggcg gataagggca gctcttccac tgttcaagga ctgtttctcc
     2521 ggcaaagaat atcggttctg gaacaaggag gtccggacca tcacgcgggc gctcggggcc
     2581 gcaagggacg cagacgtcca gatcgagttt ctcctatcca ttatcgggaa tgtcggcgag
     2641 agagactccg ccacgcggaa cggcgcggaa ctgccgcagg agatcaccgt cgcagccgag
     2701 cctgctaccc gggaccttct tctcggactc aaatgtctcc ttctgcgtct tcagcagaga
     2761 agagcgagtc ttcagccgga tgttattgca gggatcgacc gtctggagag cacgggggtc
     2821 gtcggggtga tggaagacac tcttgccggg tatcttgaaa gggaggagtt ccgcaacacc
     2881 gacgtccgtt cgcctgcagc gtatgtgcat gcgttctccc atatcacgca gcagatcgac
     2941 gaggtattcc ggtttgagcc cttcatcgcc atgcccgacc ggaccggcga gcaccatgcc
     3001 atgcgcatcg ccgtaaaacg cctccgatac accatggaag tgttccggga cctgtacgat
     3061 tgcggcataa aagagcccct gacgccggtg aagcatcttc aagacgttct cggcgagctg
     3121 cacgactgcg acgtctggct tgggtgcctg cctgaatttc tcgactcgga gaaggagcgg
     3181 cagcaggaat acttcgggca ccttgagttt ttcaggatcg tggagccggg catccgctac
     3241 ctcatcgacg acaggaacag gcggaggcag gaactccacg cactgctggt gcggacatgg
     3301 accgagcaga atgaggaaag actgtgggaa agcctccgcg agacgataag cacccccctg
     3361 ctgggccgaa aacaggatac gccggcagcg gttgccctca tcggggacgt gcgtggaaac
     3421 catcccgctc ttgcggcggt cctcaacgat gccagggcac ggggcgcaac gctcattctg
     3481 aatgccggtg attccatcgg ttacggttcg tcccagaacg aggtcgtgcg gatgctcaag
     3541 acagagaata tcatcagcgt tatcggcaac cttgatcgag acgtagtcgg agtaaaacga
     3601 gttcaaaaga cttccaaaaa caggaaacgg cacaggacgc tgctgcgggt ccgtcgcggc
     3661 ctttcacgga aaagcaggag atatctccga acgctgccgg aaacagtccg cctgacgctc
     3721 ttcgggaagc gcttactcct cacgcacggg ggtccatcct cgatcaccga taatatctcc
     3781 gaaagggcct tagatcagcg attaaagcac cttgcggccg aagctgaagc cgacatcatc
     3841 gttaccggac actcccgccg cccgttcgcg accgaggtcg acggggtctg gttcgtcaat
     3901 accggtagtg ccggaaagcc gggtgacggc gattcacggg ccggttatgc gctcctgcgg
     3961 caggatccgt tcgcgatcga gcatattccg gtcgcatacg atgaagcacc acaggagatg
     4021 catggggagg aggactgcga gcccgacgca ataaagggtc ataagattac agagccaaca
     4081 gctcagcaga tcgatcggac aacggaggca caacagagca atgtcgaaca gcacaagtag
     4141 ccgaaccgag tcaatcgcgc acgttgtgga ggaatacggc ggagatatcc gacacgcccg
     4201 gcaggtatcc actcttgcgc tccggctctt cgacgaactg cagccgctcc atcgacgaac
     4261 cgaaacggat cgcgagtacc tcgaatatgc cgcactgctg catgacatcg ggtgggcaga
     4321 aggccgggca ggccaccaca agcgggcgct tgagacgatc cggcgtgagg aacagcttcc
     4381 cttcaatgaa tacgagcggc ggatcatagg aaacatcgcg cgataccaca ggaaatcgct
     4441 cccaaagaag tcccataccc cgtttgcgga actaaacgcc cccgaccggg aactggtatg
     4501 caaacttgcg gcattgctga gggttgcaga cgggcttgac gtcgcgcaca ccgatgcagt
     4561 ccgggagatc ggctgcggca tccacccgga caggatcgtg atcaactgca cattctcttc
     4621 tccggccgtc gaagaacgga agtctgcctt aaagaagagc gatctcttcg agtcggtcta
     4681 tcgacggagg cttgtgatcg aatgaccgca ccccaaaaag ggcgggtcgt tgcattcttc
     4741 gacctcggca cgaactccgt tcgcctcctt gtagcccgtt ttgagtccgg acagtcctat
     4801 accgtgctta cgaagcagaa agaagttgta cggctcggag agggggagtt cgcccggggc
     4861 tggctcaggg acgacgcgat ggaccgcgcg gtccgtgcct gcaggaggtt caccgacctt
     4921 gcacgcagct tcggcgcagt agagtacgtc gcagtagcga cgtccgccgc acgagaggcg
     4981 cgaaactgcg cagaattcct caagaggctg caagatgagg ccggtcttag cgtccggacg
     5041 gtctccggga tcgaggaggc gcgcctgatc tatctcgggg tctcgcgagg cgttgagatg
     5101 gacggtgctg ttgcactttt tgtcgatatc ggcggcggca gcaccgagat catcctcgcg
     5161 gaccgggagc gggagcatat gctcgagagc ctcaatctcg gggcactacg tctcacaaac
     5221 caattcttcg aggacggagc ggcagatccc gtgccgccgg aggtctacga gaacatctgc
     5281 cgctgtgtaa aggacgccgt catgccaacc gcggaacagg ttaagaaagg tcgtgcagat
     5341 gttgcctacg gaagttcggg cacgattcag aatcttgcgg agattgcccg gcgttctttc
     5401 caggaccgac ttccggatac gcctccgatc ctcacccggg aggatctcag aaagaccgct
     5461 gccatgctct gctcgcttcc gctcgatcag cggaagaaag tccccggaat caatccggag
     5521 cgtacggaca tcatcattgc cggagccgcc attcttgagg ggatcatgga cgagttcggc
     5581 cttccggaga tccgcataag cgaccggggc ctgcgggacg ggctgatcgt cgattatctc
     5641 tcgcgccaaa aaggcatctc acccgcagac cggttgcagg agagaaggca gagcgtacag
     5701 cggcttcttg gcgcatgcag agccaatccg gagcatgcgc agacggtcgc acgcctctcg
     5761 ctggaactct tcgacagcgg agtacgggcc ggtctgcata cgttcggcgc tgcagaacga
     5821 gaactgctcg aatatgcagc gtacctgcac gatataggag aactcgtctc gttctccaac
     5881 catcacctcc actcgcacta tatcatcgga aatgcggatc tgcccgggtt tgaccggcag
     5941 gagatcgata ccatcgcaga agtggcccgg catcaccaga agaaatcgcc caaggcaggc
     6001 gcaggatcga aggcaatacc aattctctcc gcatttcttc gccttgccga gcacctggac
     6061 cggggccatg ccggcctcat cacccatgca cgcatcgcct ctgcagacaa aagagaggcg
     6121 accctcgaga tcgtgagtgc aacagattgc acccttgagc tctcggcgat tcggtccgag
     6181 gggcgcacca ttaagaagat cttcggcagg gatcttgcgg tccggtgcgt tggtatcgaa
     6241 tgagatccgg cagccgccgc tttccgttac cgtctgcaaa caccgtgaat aatcagctcc
     6301 gcgatac
//
LOCUS       DAJB01000255            3728 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_81970, whole genome shotgun sequence.
ACCESSION   DAJB01000255 DAJB01000000
VERSION     DAJB01000255.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_81970"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          685 a         1151 c         1136 g          754 t
ORIGIN      
        1 gtcccgtccc cgaaacactc ggcccagact cccgtgctgg aatggttcgg ctccgggtcg
       61 tcgaagacat ccgcagcctc ggccccccag gtatcggcga cgtgaaagta gtacccaatg
      121 aggacgttac tgtcgaggaa ataaggcagg ttacctcctc cctgccggag actcactgca
      181 cgcgagatcg cgggagatgc gcatcgccat atcgacgaga gccgagaccc tgccgtcgga
      241 atccgggggg agagaaggtg gattatcgcg ggcgatcgcg tcccgatcgg tgttgaagcg
      301 ctgctccatg gtattgcaga actcctgcaa ctgctcctcg ctatacacag ccgggatcat
      361 atgcgtcgcg acgacgtgag agacgaagca gagcgcatcg cagccttcgg ccgcaagtgc
      421 gggggcgtgc gaggggttgt ggtggagcgt agtgcggtat gcgtcgtccc acgccaggta
      481 tgcgtcgtag aagatctcaa gggcgggcaa cggatacttc gcgatgaagt tgtccaaaat
      541 ctcctcgacg gtggcaatcc gctctgcctt cagggggtgg agcgcgccgg caacctccgt
      601 ggagagagga aggattcccg tacagaacgt gcgctcgaag ccgtcggcgt gcaggccgga
      661 atgttccaga tacattctct cgcggcggtt taaccggaag tactcgaggg gaatatcttc
      721 tccatcggaa aactccttcc agaggcggaa gtagtgggta atgtcactga tgagatccgt
      781 cttcttgaac ttcatccact ggagcaccga ccagaggaga actcgctcgt agggcgtaaa
      841 gatcgtgaga tctgccttgc tcctgggtct gtattcgtac gactcgtagg tatttcttcg
      901 aaaaaaaccg cccgcccttc tccttcccgt atgcgcattt gctgtcggtg ccttcacgtc
      961 gaaatatgcg ttcgattcgg atgttaattg gaacgccgta gcctcggctg gtcctttcgg
     1021 catgcggatg taggtcgtcc cgcagagcgt cgtttctcgc aggttgtaat agatgaggtc
     1081 cacgaagaag atgaacttca ggagtttcgt ccgaccgatg tgaccgtcgc gctccaccgc
     1141 gtacagcaac gcgttgatca acttctgctt gtgcatccca tcacctcgac cctgaagctt
     1201 ccgctataag cgggaaaaca agtctgtatg cgtgcgtatt acaggtacta aaagataacc
     1261 ctttctgaaa gtgggtttta tcatcgcacc ttcggtgctc gaactccaga ccttcggccc
     1321 gtcgctaatc gcagatatgc gtctgcgccg ccgcttcgtt gcgggtcatc tttgagtcct
     1381 gcccgccccc gtctcctccc acccgagaca gaccctgacc ggcactctcc cttcaagcga
     1441 agccgcgacc ggcgagcgcc ggtaagcttg attcacgagt tcctcgacct tctcccacgg
     1501 tgcgtcggca tccacccgga cggtcagccg gatccccgcg aggcccggtc gggcatcgcc
     1561 aaatgttgag gaaccccgca aggtcgaggt cgacctcgac atcgatctcg atcgaatcga
     1621 cctcgacgcc gtcctgggcc gcgctgtagg cgatagagat cgcggtgcag gcggaaagcc
     1681 ccgtcagcac cagctccgtc gggctcgctc cccggtcggt cccgccgagg gcgaccggtt
     1741 cgtcggctgg aatggcgaag ttccggacga tcgtggtgga gtgcgcaccg ccgttccagc
     1801 tcgtgatgca ggagagggtc cgtttgccct tcactgcatc cttccgtatc gcctccacaa
     1861 gggcgtccac cctctcgaga tcgactccgt tttgcatcaa tcgcatcaga ctcctcacag
     1921 aacgttgccg tcagatgatc ggctccggcg gttttcctgt cgatctcacg agccgcagca
     1981 tctcttcggg atcggtcgtg gggatatcgc aggcatagtt ggtgcagaca tacgccgtcg
     2041 ccctcccctc gaccgttacg atatcgcggg taaacccggc tacttttgta atccccggat
     2101 cttcctcgtc ggaggggcgg aagatgacga cggcatcggg cgtatagtgc aaccggatgg
     2161 cgttgaccat cgctctcgta tcctcctcgc ccggaacccc ggatatgatc acctcgaagt
     2221 tcggccccag catgaactcg aggccggtaa ggaagtggga gtaggcggta ggagactgcc
     2281 gaatggcatc ggcaaagacg tgccgcatcc ggttcgccgt ctcctcgatt tcgaggttcg
     2341 ccgtcatccg gccgaggacg aagagggcat acatggccac gctgttgccg gaggggatcg
     2401 ctccgtcata gatcggcttc tggcggacgg ggacgtccgc atcgtctgga acgaagaaaa
     2461 accctccctg gttgcagtcc cagtagtggg cgacgaggtc ccgggagagt tcggtggcgg
     2521 tcacgaggta gtcgggggag aacgatgctt cgtagacctc gaggagagcc cagagcatga
     2581 acgcgtaatc gtcgagggtc gccggcaggc cnnggtgaga acgaacgcgg ctcccttctc
     2641 cgccgcggtc aggtactccg ggtcatcgaa gacccgggca gctctcgcca gggcggcgat
     2701 cgtgagggcg ttccagtcgg cgaggatctt gtcgtctttt gccgggtgga cccgctctcc
     2761 ccgggctgta aagagtttct gccgggcccc ctccacgaac catgccaggt cctcttcgga
     2821 ggtcgagaat tcatgggccc aggatgccag ggggcgccgc aggcggagga tgttctggcc
     2881 cgtcctcctc ccgccgggct gctgccggta attgccgggc tccgtgacac cgaagatccg
     2941 ggagaaccgc tccccgtcct cttccccgag gacccggagg atctcatcct tcgtccagac
     3001 gtagaacttc ccctcctcac cctcgctgtc tgcgtcctcc gccgagtaga agccgccacc
     3061 gggattggtc atatcccgca ggacgtaggc gatcgtctcc cgggcggtcc tcgcgaaatc
     3121 ctccctccct gtcgcaaggt aagcctccgt atacgccatg atcaggagcg cctggtcgta
     3181 gagcatcttc tcgaagtgcg ggacgagcca ctccgcatct gtcgagtagc ggtggaaccc
     3241 gtagccgagc tggtcgaaga tgccaccccg ccgcatggca tggagcgtct tctcgaccat
     3301 cgcgtatgct ggctccttcc cggtccggtt cccgtatcgg aggagaaata tgaggttgtg
     3361 cggggccggg aacttgggcg catccccaaa cccaccgttc tccccgtcga agatccggaa
     3421 gaaggtgtcg taggcctcat cgaggatcgg ctccgcaagc tcgctctccc ccggaggagt
     3481 gcgggccgcg ctctgcagcg cctggagcac ctgatcgccg gcgttctcga gttcctgccg
     3541 ctggctctgc cagatcttgt tgatccgggg gacgaggtct aagagaccgg tcatcccata
     3601 ccggctctct ctggggatgt aactcgctgc aaagaacggt ttttgatcgg cggtcatgat
     3661 gatggtgagc ggccaccctc cggccccggt cagcgcgtgg gctgccgcca tgtagacctg
     3721 gtcgatat
//
LOCUS       DAJB01000256            4659 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_128184, whole genome shotgun sequence.
ACCESSION   DAJB01000256 DAJB01000000
VERSION     DAJB01000256.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_128184"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    4420..4515
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          938 a         1371 c         1249 g         1005 t
ORIGIN      
        1 ttcatcttag actatatcta ttttttaatt tacggaggga aaaatcacgt gaagccccac
       61 tatatcatca tattgctccc gtttcttctc ctcaccgcgg ggtgcatcga gcccgtggcc
      121 accgacaccg caggggttac gccgccaccc gcgatagacg ccacaaccga actttccccc
      181 gataatgcac catcactccg ggattcagcc ttcatcagga tcgaggaatg ggaagacccc
      241 gccgaataca cgagtttctc cccgccgtca tacatccccg aggagtatac tcactacggc
      301 ctcctgatag ccactgtctg gaacgagact tacggccact acacgacgag cggcgagacc
      361 ctgtggtacg ctcacgacga tacgatacag aatgcgtcat ggacctccga tatgacgctg
      421 ctcgaaatca cctggagcaa ctgcgggtat aacccctatg caaaagatca gattgttaac
      481 aaagaaccgg aatccgttga tataaacggg tatgcgggac ggatctacga aaccgaatcg
      541 gaccggctga tcgcctggac ggcggccgat aacgctacgt atcgggtacg tgggtcgctt
      601 gaacgcgacg aattggtccg ggtggcgcga tcgatcgctg gttaatcggc ggataccatg
      661 gaggggagat gaaccatctc tgcaagccgc tattacggtt ccgaaaaccg ctgtgcagca
      721 aaatcactca cggagaacca tcaaagagga gaacaggttc tgccgcgctt caggaatgaa
      781 atcaggcaca cattaacaac aggaggttgt aaatgggccg cataaacacc tcatgtatct
      841 atctcctctg cgagcacctc tcccggagcc gactgcgctt cgcaatcccg ccgcttgttg
      901 cggtcatcgc gtgggccgtc tgggtaggtg tcgccggcat cgcctcaggt catccggggc
      961 ccgtaccgct ctttgctctc ctcaccctct ttctggtgat cctgactctt gcagttgcgg
     1021 gggccgctac gccgttcccg cttgtcgcag gcgtcctctc gtttcatggg cgaattcagg
     1081 cgatgctcgt taccgccgca gcaatgaccc cgctggtatt ccccctgtcc atagccccgc
     1141 accgctggag aggcgagccc ccgctgctcg cggacagact ggcacttctc gggtggttct
     1201 tcgacggcgt catgaacggc ctgccgctcg ccgaaaacac acaagtatac gatctggtgt
     1261 tttccgcgtt cacgttcctc gggttctacc tcgaatgtgt gctggtcgcc accgtcttct
     1321 acgttattct ccgggtggtc gtcatcctcc gccgcacgaa caccggggaa ggagggagcg
     1381 cgaaatgacc gcagacacag ggtaaaacca gatgagatcg cgggcgttct gggcgtactc
     1441 tgcggtttat tccagtataa cctctggatc gccgtgctca tggcgatccc cttcacgttc
     1501 ttcgcagaac cgaacgccat tgggagcatt catagtcaaa tccccgctca tgctacggac
     1561 ctggctcacc gccgggatgc tgccgctctt catcatcttc ggctactacc tgtttacccg
     1621 cgaggagatg cccgcagagg aactccttct gagcagatcg ggacttgccg cctcagcctc
     1681 gggttttctc ctgtggcttg cggtgctggc tgtcctggag gtttccgggg ttgccgtggc
     1741 gtatccctat aacgtcgccg gtggttatgt cgtcgtgctg atccggggtg tgatcttctg
     1801 gaaagcctgg agccggggag catgagtccg gtcgccagaa ggggaatcat gaaggtcctg
     1861 aaggttattg tcgagaagca ccctgacggc tatgtcgcct accccctggg tctgaaagga
     1921 gtcgtcgttg cagaggggga tacctacgaa aaagcgctag ccgaagtgaa atccgccatc
     1981 cagtttcaca tagagacctt cgggaacgat gcgttcgaga acgacgatat catggagacc
     2041 ttcgttgccg aagtcgacat ccgggtctaa agtcgtgtgg ttaccggcga ccggtctcgc
     2101 gcttccccgt tcctcttctg gagccctcga taccctgctg attcacgcaa agtgcgagcg
     2161 acagcgtagc tttgattacc acaggaacga cagaccgaag ggccggagct cgatcgccga
     2221 aggtgcgagt tgcgagccgc gatgatccgt cgggaccgga gcgtgagcac cgaaggtgtg
     2281 agccgcaaag aacacgaacg gggaggaggt gcttatcacg gacttcggcg gccttcgtta
     2341 gaatagtctc ccttctctgc ccccgtcccc ggagcgagat cgatcgcttc tcttttgcga
     2401 acggctgacg gaatgtaaag aaattggaca gaattcacac ccaggtagat gatggatgaa
     2461 gcagacagcc acgctcgagg agcatcgggg tagttcaccg ggaataacgc tgacgttgcg
     2521 actcacggga aaaggtgccg atagagttct gcaatgagcg gatgcacctc tccagacttt
     2581 cggagtggtc tgatgcaacg tccggtctcc atccccgttc ggcatcgttg acgaactccg
     2641 aaagaagcga caggtacgcg ggcccttctg cccgccggcc gcccggcgtg gggacgatgc
     2701 ccttccgaat cgccgatgaa ctcgactctc ttgcgcagtt gatcaggtac tccttcgggt
     2761 aatcgatctc ctcgggcgtt ccggttatcc gtgcctccgg gacctgcaga tatctcgcca
     2821 gcctctccct gtcggccagg atccaggcct caacctccct cacggccacg cggaagttca
     2881 tgaaatgcga aggggcaggc agccagttcc ggacagcttc cggcgcgcat ccggtatcgt
     2941 gatccagatc gacgaccaca atccagggcc gggtttgtgc ggaagcgttg aatccctgta
     3001 atctctctag caaataggat ttcctctgcc tgtagaggtc tccaggcagg gccccgacgt
     3061 gccggagcaa ccgtctcaca actgcttcat ccagcgatcc ttcaaaggcg cactgaaatg
     3121 ctatcccatc gtccatccta ctctccgaac cgggctaact ctgcagtgtc gccgggacag
     3181 gtctttggga cgacgacatc cgcaagggag aggccgccct ttaacaggtc gtcaacctcc
     3241 tcgatatcac ctgccggtgt aaccgttgta ccctcctccg agggccggag cagcagcacc
     3301 tcatcaagcc cgataccctc gtcctgcagc agttcagagg aatgggtgct gacgagcacc
     3361 tggcgttcac tcgtcagctg cacccgggag aacatctgcg gaatgtacct gatgatcgca
     3421 ggatggagag agagttccgg ctcctcaagg aggagcggtc ctccgtcatc caggagtgcc
     3481 cagagaaggc cgatgagcct gagtgtcccg tcggagagct gttgctctgt ttgccaggct
     3541 ccctgcggcc tccagtgctt atacctccca cagaggtgcg gtttgccttt gctgtcgaat
     3601 tcaactttaa tctctcttaa ttgcggtacc gcaacgttga gcgtcctctc gatccggtcc
     3661 agcatggaat cccgctcttc ctcggatctg ctcatgacct gttcgagaaa gtcgccgcca
     3721 taagggtcgt ttttgtggcc gaacgaacga tccggttcac ggaccagctg cgggacgagg
     3781 tgcaggtagc ggatgttttt caggaagtcg gcaacctccc gaaattcttt gttgacgttg
     3841 acctgctcca gatacgtctg ggttaacctc tcggggtcct cctcatcctg ttcggagggg
     3901 cgagcgatca cttcgtcccc gcacctcgca acccgctcct ttgcgatgac cggctggttg
     3961 tgctcgtcca gcccaaacgt caactcgtat tcccagagat cgccgcactc cacgttaccc
     4021 agccgcacca ttacgcctat atcggggtac cgtctcgcag agagtgccct gaggctggag
     4081 acgccgcctc tttgtttcac cgctccctga aatccgccgc ctacggacac aatatcgtgc
     4141 aagaatctga acgcatctaa aaagttggac ttcccggatg cattgggtcc gacaaggaaa
     4201 acccggtcct gcaggtcgac atctacccgc tggaagttgc gccagttcca gagagagata
     4261 tgagtaaatt tttccggcct acccacagat ctggagcagt tacccatgat agtaatcgat
     4321 tgaagccccg tgtatattac ccttttcgaa gaccggaggt cttctcacgc ttcctgctcg
     4381 aagcctgacg acttggtagt gctccagcat atacctgatn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnntgcct gtccctgagt tcgacctttt cgtatgaaaa tgggtcacct
     4561 acatattttc atccggtatg cctgtggccc cacgggcatc atgtggtttt tctcacgcat
     4621 gtcctcatac cgtagaccgt ggtggctatc gccatgggg
//
LOCUS       DAJB01000257            3974 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_110775, whole genome shotgun sequence.
ACCESSION   DAJB01000257 DAJB01000000
VERSION     DAJB01000257.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_110775"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    801..882
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          866 a         1215 c         1144 g          667 t
ORIGIN      
        1 ctcgtaagat atatcttact cgatcccata tgagcctttg catggcaccg ggcacacgcc
       61 cctcaggaac ccgtgggcgg acgcctgccc gcccttctct cttcagacag ctcgttatca
      121 tagcgatcga tgtcgatcat cgaaagaacg cctatgataa cctgtgagat gctgcgaaag
      181 actccctctg ccactcacgt cctcgaactc gtgtcaggat ccgaacaatc tcggcggccc
      241 ggacaccctc gcatcgatag gctcaggcac ccggtatacg gagcaataac gctcagggct
      301 cattcccggc cagccagtcc tcgtagcggt cggcatgctc catcatccag cgggccatcg
      361 caacccacct ctgcggcggc atcccggccc atgaagaaca ggggagacta cggggggacc
      421 atgaagatcc tggtcatcat gggaagcccc ggaagggcaa cacctaccgg gcggcaaaga
      481 ggatcgagga ggcgatgcag tccctcggcg acgtcgagtt cgagtacctg atgctcaagg
      541 acgtcgggct caagacctcc tcgcgtgctt cgagtggggc gaggagcgct gccccgtccg
      601 tgacgacgcc cccgccgtcg gacagaaact gcaggatgcc gacggagtga tctttgcatc
      661 cccggtctac ggtctcgccg tcaccggcct catgaagacc ttcatcgacc ggttctccta
      721 catcttcgtc cccggttctt cgacaagaaa gccctcctcc tcaccaccgc cggcgcggtg
      781 gggggaaaag acgtcctcga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagcggcg gccggggctc
      901 cggagcgtca tcatcttcca cggccagcgg gcgacgttcg acgagctcgc cgacgacttc
      961 ccggccgacc acgcctactg gaaggagcag ggctggctcg atccaggagc ccgctactac
     1021 acgagcgccc gggtgaaccc gttctacgac gccgtgggga gaatcgtgga atgggtcgcc
     1081 aggcgaagga tccggcggga ccttgccgcg atgcgctgac cgggtgcccg gccggtcgat
     1141 gagcagcggt tcccgtggaa aaaacccgac catatcggtc gccggatgga tatacgccgc
     1201 catcggcctc cccgtttccc ggcaggggac agtccgaaaa aggaccgtcc agatcccctt
     1261 tctggccata cagactcata agaaattcct gtttttatta taaaaagttg agaataaatc
     1321 accgtaaagg atatatattt atatcgccat ccccccattc accggctacc ctatgaccgg
     1381 gtcttaaatc agaattcgca gtacgcctgc cgggtgctcc ggcgagcacc tgcttcagtc
     1441 agcccgtttg tgcctaccgg caggtatgca ccggcggtct cgcaggcggt gagcgatcag
     1501 cagggggaaa aatgagaaag cacgcctatt tggtactatt gtgcgcagtc gttgccatcg
     1561 gactgctgat gccgccggtt atggcggtag agactgcgtc gggggaggag atttatcgcc
     1621 cctttaatgg agcgcaggaa caccccgata tcgacggcaa catgatcgtc tgggacgaca
     1681 accggtatga tgagttgaat aatggagtca aggatatttt cctcgcgacc gtagaggagt
     1741 tccagagtac cccgccgtac agttatgttg ggacaaggat aacagacgat cccgcctcgc
     1801 aggagaaacc atccatctcg gaagactaca tcgtctggca ggataaccgg cacgggaact
     1861 gggatatcta cctctatcag cgctctacaa gcacgacaac acggctcaca actgactcgg
     1921 gcaaccagtg gctgccgatc gtccgcggga actacatcgc ctggtacgac aacagtagcg
     1981 gcagcaccaa catcgtcctc tacgacatcg ccgccgggga cgtgaaggat gtcatcaact
     2041 gtgacgcaaa gacaacgata ccctacgggt cgaccgggtt caaacccgca ctctcggaga
     2101 agtatgtcgc ctgggtggaa gagaatgatg ggagggtcta ttactacgat atcgaggccg
     2161 aagacccagt aggcccggta tccacgagca tggcgactca ggcctggccc tcgctatacg
     2221 ggagcatcat cgtctgggag gattaccggc atggaggcgt aaaccctgac atctacatgg
     2281 ctgatctcga caacccgtcc gccgaagaga cgcagatcac ctccgacacc ggggaacagg
     2341 tctcgccggt gatcagcggg agtatcatcg cctgggagga caagcgggtt ccggcgcgga
     2401 gcatcttcat gtacgacctc tccgtcaggg aggaactgtc cgtagttgaa tccgcagaca
     2461 actacgatga atacctctac cccgccgtca gcggcaatac gatcgtctgg cagaggggct
     2521 caggcactaa ctcgaacatc tacatcttcg cctacgagcc tggagcaccg gtcgaaccgg
     2581 tcgcaatgag tatcggggta gaaccgtcca cgcccacaat cgcggtcaac gacacggtga
     2641 cgttcgaggc aaccgtccgc aaccagttcg gcgatcagat gaccggtgtt gagatcgcct
     2701 ggacaagcga caacgagact gtcgggaccg tcgacgatac cggcctcttc accgctcatg
     2761 ctgagggcac ggcagatgtc accgcaaccg ccggaggcgt ctccgggagt gcgaccgtta
     2821 ccgtcaacgc cgaagaaccg gtcgcaacga gcatcgaggt cgtaccgacc gcggtcaccc
     2881 tggagatcga cggcacagag cagttctctg caaccattct cgacctgttc ggcaatgtca
     2941 tgaccggagt tgcggtcgcc tgggcaagca gcaacgagac ggtcggcact gtcagtgaga
     3001 gcggagtctt cactgctctt gccgggggca cggcgacgat caccgcaacg gcaggcggcg
     3061 tatccgggag tgcgacagtc accgtcaacg caaaagagcc ggtcggaccg gttctgacac
     3121 gtatcacggt cacgccgccc acagttaccc tgggtgtcgg agatatccag agattcatcg
     3181 tgacggccta tgaccaggag gacatcgtca tctccgccgg tgaggtcgcc tgggcgagca
     3241 gcgatgacac cgtcgggacc attgacgcaa acgggctctt caccgccctc gagagaggca
     3301 ccgtgaccct caccgcatca gctgacgggg tatccggaac cgcctgcgtg acggtcaaac
     3361 atcgaggtca aacagaccac ggcctccatc gaggctggaa agacccagca gttcacggcg
     3421 accgtctttg acccgcgcga caacgagacg aaccgggtca gggttgccag gtcgtgttcc
     3481 gacccggacg tcggcatgat cggcaggacc gggctcttta cagcgttcgc agagggatcg
     3541 acagacgtaa ccgcctcggc aggcagcgtg gaggggaccg ttgcggtcac catccccgcc
     3601 gattggcttg aggagcacac catgacgccg gagggtgtca ggctcatgcg ctacgtcaac
     3661 ggcggctggc agatcctgaa gaccgaggtc atcggtgagg agaatgggaa gtaccgcctc
     3721 cggacaacca cgcccgggtt ctccaccttc gccatcgctg cagcgccgat gaacgtgaca
     3781 acgatcaagg agacgaacgt cacggccacg gcaaccgtga ccgaagaatt gacgacggag
     3841 ccgccaccgt gccggcgacg acacctgtgg ccccctcgtc tacgcgccac tccacccaga
     3901 ggccgacgcc tggatatccc ccggagtatg cccacccaca ggccctgcac ccatatgggt
     3961 acatgaaaaa ttcc
//
LOCUS       DAJB01000258            4002 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_32104, whole genome shotgun sequence.
ACCESSION   DAJB01000258 DAJB01000000
VERSION     DAJB01000258.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_32104"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT          764 a         1186 c         1143 g          909 t
ORIGIN      
        1 cacctacata ttttcatccg gtatgcctgt ggccccacgg gcatcatgtg gtttttctcc
       61 gctccggcct ttcatcacgt ctttccgcac atcccgctat cggtgagccg gttttccttt
      121 gcggaggtcc gggctgcttc cgaaaaaggg gagtattcag ttccccggca ccatccggcc
      181 gaggtgatca agggtatcaa gcggtaggcg cttcggtgga agatcgagaa cctcgcgtgt
      241 ggggcgcagg actgccggaa ctgcgtgctt taaaacggga tagcgaagac cggatcgccg
      301 atctcatgcc cggggcgggc cgggaggtcc gtctccggta cgaccctggc gaggtggccg
      361 cggtctgcac gagatcaaaa agccgggatc gaccgaccgc cccggaagag ttcacccgac
      421 gcgctttccg ggatgcggtg agaataatag atcataatcg acacttttat agataatcat
      481 cgagtatgat cgatagatgg acgagaggct gcagatcgca ctcgagagcc agaacccctg
      541 gtggttcgga aaagagttcg aaaccggcgt tgaacgactt tctttctttc ctttcctgaa
      601 gcagtacctt ggtgcgccgg aggtgctcct ccttgtcggt gcacgcagga cagggaagtc
      661 gacgcttgtg taccagatca tcagttccct tctcagaagc ggtgtgcccg gcagggccat
      721 cctcttcatc accatggacg aaccactcct cctctcgatg gcagacgatc cctggctgat
      781 ccgtcgcatt gtcgaggacc atcttgcccg ccaccccgaa ctcgaacggc tctatctctg
      841 tatcgacgag gttcagcact atcctgcctg ggtgcccacc atcaaaaccc tctatgacac
      901 aacgagtaca gtcaaatgta tcctgaccgg ctccacatcc gcgctgctca ggcaggagag
      961 cagccgcctc ctctcgggca ggtattttcc ctgtaccgta tatcctctct ctttccctga
     1021 gtttcttcgt tttcagggta ttctgaaccc gacaatcatc gagaagaggc attattttga
     1081 gacctacctg aaatacggcg gtttccctcg cgtggtcctt gaaaaggaca gggatctgaa
     1141 gatgcagata ctcaaaaatt actacgagac gatctatctc aaggatatca tctatccaaa
     1201 caggttgagg aagaacagcg aggttgtaga cctgctctat tatctcatat caaatgccgg
     1261 caacgccgtc tcgtacaacc agattgcccg gaccctcaat atcgcaccgg ataccgtgag
     1321 agagtacatc gagtacgccg ggaacgcgtt cctcctgtat cctctgatga aattcgatta
     1381 ttctgcaaaa aaacaggttg caaacccaaa aaagatctat ttcgtggacc ccggcctggt
     1441 aaacgccgtt gcatttgcat tctccgaaaa tcgtgggaga ctcctggaaa acattgtctt
     1501 catcgccctg atgaagaagt acggtgaggt cttctaccac cgggaccgct acgagtgcga
     1561 cttcctggta cggtcaggcc agacgatcgt ccgggccatc caggtgacgc agtccctgca
     1621 ggatgacgtc acgaggcacc gggagatgcg ggggcttgtc gaggcgatgg tcgcacatgg
     1681 ccttgctgag gggacggtcg tcactgcgtc ggagtcagcg gagacaacca tcgatggtcg
     1741 gacagtccgc atcgtctcga tcgctgaatg gctggaagag ttatgacgtc ccttcggggt
     1801 cgtcgtaggg ttgccagccg ctcgcagcgc cgtcactcca gattcgaggt actgcgccgt
     1861 taagatagac cgtgtggggt gaaagcgatt tttggggtta aagttccagc aggaggtccg
     1921 gatcgggttc agttgatacc agagtgatgt ttgtaatttt gtggtagaac ctctgagggt
     1981 aatggtaggt tgtcttcccg ctccggcttt tttgcgtgaa gggtttgagg atgtcctccg
     2041 tctcatactc gagcgagtat gccgccccga ttttgagggc aggcccttcc ttcgcccgga
     2101 acatgacctt cctgaaaccc tctgttgaat ggtcgcagtc cgagtaatcc gaggcgaaaa
     2161 caagcccatc ttttcggatc ctatcgatca caatcctggt tcgtagcatt ctcttcaccg
     2221 gttctgattt tgattgctgc caggcattaa gatgtgccgt gcggggtgaa agtgctcccc
     2281 ggagccccgc tctagataac ccccatctcc gacaacctcg tcggcaggta ctccttcgtc
     2341 acgaagtcga gcccccggct cgcgaacgcc tgctgttccg acttcacctt catatcgagc
     2401 tgcaggtgga tctggttatg ccagtaatcg gtcgcaaacc tcggatcggt cagttccgcc
     2461 tttaaggcgt tgatgtcctg ctccgtgagg tggtcggagg ggaggttgta gtcggatatg
     2521 tcggagggct gcacgccgat gaactgcgcc tggggggttg ccagaagttc cgacatgtgg
     2581 gcgctcttga tcgagccgta ggcgacgctc gcgtagatcc ggtaggacca ggggtcgccg
     2641 tcggtgaaga ccacgacggg cagaccgagc gagtcgttga gtttccgcag gacccgccgc
     2701 gtggagcgtg ccggctgccc tttcaggtgg accaggaccg ccccgtactc ctcgtcgaac
     2761 ccgttctcca tcagacgggc gtacataccg ccggtctcga tggcgatgac gaacttcgcg
     2821 tcgtgctcga tgaagtcgag gctgtcgacg ttgttcggga tggggtaacc cgcctccccg
     2881 acatcctcct ggcagtgcat ctcccgcacg ccgcgccggg tggtctcccg tatcctcagg
     2941 ggtccgaaga gcgaggcccc gtcctcctcg ggccggaggt ggaacgcctc ccgctgcacc
     3001 tccgtgatga tctcgagatc ctcgattaag aggttgctct cgtcctgggc ggcgaacttc
     3061 cccttcttcc agccctcgga gatgtagtag agttctctta atgtcgacga ccggttctcc
     3121 acgagttgct tttttaagaa ccagacgagg taggccatct ttaagaggtg ggccgcgcct
     3181 ttttcggtcg tagccgcccg ggtgctctcc ctatcgccgt acttccagac ctcgctttcg
     3241 tcgtcgtatg caatattccg cttcgtccgc gtcggcagcc ggataaacgg cacgatgccg
     3301 tcccgtatct ggtcgtacca ggtgcgggcg ataccgacga gccgttcctt tgccagcgca
     3361 tccatctctt ccctagacat cgagatccac caccactttc atgccgtcct ccacgccgcg
     3421 cagatcgagc gatccgtcgc ccgcccccgt ataggcgacc tgccaggccg cgccgggggc
     3481 aagcgtgcac cgccagacct tgttgtactc cccatcgata tggtccgtga agtccggccg
     3541 gggctctgca tccgccgccg catcacgcga gaggttgtag aggacgaacg taacctcccg
     3601 cgcggtgtag ttgttcacat agatgtggac cttcccgtcg ctcgtccgtt tcttggcgac
     3661 gatcttccgc atgatctttc cctcgatcgg ggaggtgtcc accaggggaa gctcgacgat
     3721 ctcgctcact ttcgcggcga tctcggggat gatcgagcat atcgcccggg cgcggtcgtc
     3781 ctgctgcttc tttttgtccc ggcgggagag gtagctcttg agttcccggc cgagttcctg
     3841 gagcacgagg acgacctccc gctcgatctc cgggatggac gcgaccgcgt ccttgctctc
     3901 gctcgtgaag gggacgttcg tggatgccac atggaccatg atcagcgccg gacccatggg
     3961 gaggccctgc tgcgagaggc cgtaaccctt ccagttcacc tg
//
LOCUS       DAJB01000259            6110 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_149413, whole genome shotgun sequence.
ACCESSION   DAJB01000259 DAJB01000000
VERSION     DAJB01000259.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_149413"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1173 a         1908 c         1761 g         1268 t
ORIGIN      
        1 tatggatcta cgggcgtaat gtatgttaat gaaaacagga aataagccag attcagactt
       61 agttacccag ttaagaacac aagataccca gatagaatca gagagagaac gcattttatc
      121 atcacttcca ggtagtctcc aggatgagat tcagagcctt ggacagagaa ttaccccaga
      181 gaaacgagag gaactgattg tcagggtatg cgaggtccac ccatttaccg ctacggaaat
      241 tagtatcttg ttcagaaaga cgcggacatg ggctaaaaac tgcttaagag aactcgttaa
      301 tgcagagagg gtgtccctga caattcctga caagccaaac agtaggaatc aggcatacca
      361 tgcgcctaaa tccggtggac aacgaaatat tttcgagtgg gagtaactct cgaggaaact
      421 caggaagaac cttgaaagta taagggttct agaaggagtt gtctcaacga tgagggaagg
      481 gggggagcgc cagcaaccta tgtgtttctc gctccgcctc gcgtcactgc tgtaagttga
      541 aaactctcgg ggtagcctca caaccccatc cggcagaggt cccgcatcgc ccggaggacg
      601 tagagagtct cgtcttcgag cacctcctca tcgacgacaa acgagacgaa cccctggcgg
      661 gcaaagagct cccggacttc gtcgagatcc gtcagcgacg agaccaggag gaggatccgc
      721 ccgaagggcg agagcaccct tccaacatcg gcgacgaacc gttcgatgac tacccttcct
      781 gtcggcccgc cgtcgagggc gtactccagc cagtcgtcga tccgctcctc agggagcgtc
      841 gggaggtaag ggggattgaa gaggacgagg tcgaagaggc cggatagccc cgcgaagaga
      901 tcggtccgga ccgccgctac cccgcgggca cgggcgcacg ccaccgcgtg ggggttgatg
      961 tcggtcgcga ccaccgccgc cgcccggccg gcaagcccgg ccgcgacgta gccgctcccg
     1021 gtcccgacct ccagcacccg gtcggtcggc cggacctcgg caagcgccgc ccggaggagg
     1081 agaaacgagt cctcggcggg agagtaaacc tggtcgggga gcatcctcaa ctcccgctca
     1141 tcttggttgc tatcagcgca aactcctcga gcgtcaggtc ttcgggccgg cgctgcagga
     1201 ggtcgtcggg cagacccgca atcgcccgct cgatcgcctc gggcgcaaac gtatccttac
     1261 cgctccggag ccccttccgg acggtctttc tccggtggga gaagagcacc cggacgacgt
     1321 cggcatagac cttccggtcg gcgatcgggt acggcggctc atgcggcgtt atccggacca
     1381 cccaggagcg gacctcgggc ctcgggcgga aagcgccggg cccgagttcg aggagcggtt
     1441 ttacggaagc ataggtctgc accatcaccg agagccgccc gacgttggac gtccccggcg
     1501 gcgcaatcat ccgctggccg aactccttct ggtacatcag gaccgcgacc tcaaagccga
     1561 tcccaagcag ccggaacgtg atcttcgaag aaacagagta ggggaggttc gcgacgacga
     1621 tatcatacgg cgggaggtcc accttcttcg cgtcgcctct aacgatctcg aggcggcctt
     1681 cctcgatctc atcggcaaag gcgacctcga gttcttcgac aagagccgga tcgatctcta
     1741 ccgcaatgac ctctgcaccc ttatcaagga gggcacgggt gaggatccct tcaccgggtc
     1801 caacctcgag caccctccga ccggaaacgt caacaaaacc ggcaatcttc tcgacggccc
     1861 gcttgtcgac gagaaaatgc tgatctctag gagcactcat ctcgatgtga agatatgata
     1921 cttctcatcc ggatcctcga tctcatgcag gatccggctg acaatcatcc gatccgggtg
     1981 cgggagcgac ttgatccgcc cggatatatc ggcgaaactc tcgaacggct tcttctcccg
     2041 ctgctcgatc acttcccaca acagcttctt cccgatgccg ggaagcaggt gcagcatgtg
     2101 gaacttcggc gtgatggaga cggatttgtt gaagaagtcg acgaaccgcg cctcgttctc
     2161 gcggacgatc ttctcgatca caaacggcag ttccagcttc gccgtcgccg tcagatcttc
     2221 gtagctcatc cgccgtttga cacgctcgac cttctcccgc tccgcatcac cgatgtagac
     2281 acggtcatag acctggatat ccgcacccgg cttcgggacg agttcaagca gtttgaactg
     2341 gtccacgccg actgcgagga taaccggttc gcgcttgtaa actggacgct ggtcgctcgc
     2401 ataccccata ggaaggatat cgatgactac cgcattctcc tctttccttt cggtcttcat
     2461 tggcaccacc ccttcaatca gaagtgggtc attaccagat cgaggatctc gtccagttct
     2521 tccgtcgtga ggttgaaccg ttctttggcg tagatggctc gcagttcgtc gcgggtccgc
     2581 ggcatcaggt ttgcgatgcg gaaagcgatc tcctctttca tcttctcgag ttttacgagc
     2641 tcggcgacaa ggtcacgggc cttctcagag gatgtctttg agaggtgatt ggcatgctcg
     2701 atactcttac gaagctcgta ggacatctct tctctggact cgagccggac tgcctccacg
     2761 gaaaggagtg tatcatgcag ttcggggagc agcatccgct cttcgctgac tattcgtttt
     2821 accttcatgc caatcactac tgctgaggat tatatcttct gcgcttttag gtgttgcggt
     2881 ctcgcaatca cctgttttat cgtatctcca tccctgactt cgagaaccca tgcgcgtccg
     2941 cgctgcccga tgactgtgcc ggtcttcccg tggaatctcc ggtggggcat gcccttctgg
     3001 acactcggct cgaccacgac atgtacccgc tgaccgagct cgaacacctg aatcaccgag
     3061 ctgaccgggg ggataccgcg ttttctgagg tctttcttga acttataccg cgtcttcttg
     3121 cgtggtccat tgtgatgtgc catgattact caccatattc gttcgctgtt tcgaccagca
     3181 ccacatcgag gctgacaaca ctcgcggtgc gccccaggat ctgggcaagg ctgggctggg
     3241 ttctgccact gtcactcgat atcaattctt tgatgtagag gcccgcttcg ccgacgactt
     3301 caacccagaa tctgccgtcc tgcgtgcctg tacacccgat atcgagaacc tgccgttccc
     3361 gaactaaatc tgcccgtcgg tgtgacaccc ggttgggggt gcgctgccgt atcgttacac
     3421 cttttaactg atcgagggcc gctctgaact catctggcgt tacagaaccg tcgatctcga
     3481 ccaggatcct gtatttttta tgcgctttgt cggatttaag gctttgcaca accttccggt
     3541 ccgcccatcc ggtcagatgg accgctaccc tgccttcagc gctgcggttg attgtttctt
     3601 cgagcgctgc gaggtccacc gaccgtttca ccggcgtctc gacctccatg acgaacgggc
     3661 ggcccgtccc gagcatccgg gcgtcgatat cctcccggcc agcaccgtgc aggacagcgt
     3721 tctccgcatc gaaggcctcg atcaccggcc ggccgatcag ttcctcgacg gagtcggcat
     3781 actgtttgcc ggtgaagttg cagagttcgc accctgctcc ccggcacgtc cggcaatccc
     3841 agtgcgtctg ggggatgccg cgctcgtact ttatgtaccg gccgaagaag aagacaggat
     3901 tgacctgcac ctcgacggtt ccttccgcga ggtcgaggat cgccacgaca tcgggcttct
     3961 tgaggtcggc cttcttcccg gtgagtgccg agacggtctt cccgacttcc cggttcatct
     4021 ccgctttcag cggttccggg tcatggaggg agagatcgct ccagaccatc tcctcgctct
     4081 ccgcgacaag cggcggcact ttcgtcccga taagaaacgt tgaaaactcg atgccgcgca
     4141 ccttttcgac gaccttcgcg gcccacgcat cgatgcgggt gaactccccg ccgcagatcc
     4201 agcaggactc cggttccggt tcgtgatgcg gctcgttcgc cacaagttcc cgggtgatcc
     4261 ggagtgcccg gccgcgttcc tcgttcgtca gcccaaacga ccgcttgccg aagaaacgcc
     4321 cgaggcagtg gttgcaggta tcgccgtatc caagaattgc ttctacctct tcgatatcca
     4381 tcaggattcc ctccggtcca tctcgtttaa gaggacggtg attgtgtggt cggcatgcag
     4441 cgaccgtggc cctaccgagt agcaggggta gccctcgatc agctcctgct ccccggcggt
     4501 aaagttgtgg tgatcagaga ggaggcaggc ctccgggagc gccgaaacgg tccggatatc
     4561 gtcccccgcc tcgtcgagga ccgcaaacgg gatctcggca agaagccttg cgagtccgcc
     4621 gcggcggatg tagacgcccg gggtcgactc acggaactcg tccccgcagg ggatcgcgag
     4681 cgccttcttg atcagggcgg cgctgctccg ctcgtccggc gagaggtagc ggacctcgct
     4741 tccccggaag aggacggtct tctccggacc gggctccccg cagaggatca ggtaacactc
     4801 gacgtcgcgc cggaggtcgt gggagagaaa gaacgaggag ttgatgcagc ggcagaggac
     4861 gtccatcctc cccgcactcc cggcaagatc attgaggctg aaggtgccgc tcgttgcggc
     4921 aaggtggcct acgatggcaa accgtttcat ctactgggtc ctgccgaact tcttcatcat
     4981 gcgctgcatg ttgaatttgc cgctgcccat gccccgcatg ccttttaagg cccgctgcat
     5041 gatcttgtag tatttgatga ggtcccggac atcgtcgggc gccaccccgg caccccgggc
     5101 gatgcgctgg atcctcgatc tcccgatcac cgatgggtcg tcgagttcgg ggggcgtcat
     5161 cgagtccatg atcaccttgt agcgctgcat cttggtgctg gtgatgtcgt aggcgtcgtc
     5221 cgggatctgc atgcctccaa gggggagcat gctcatgatc tgcttcaagg gccccatctt
     5281 gttcaaggcc tcaagctgct tgtacatgtc ccggagcgtg aacctgcctt tgagcatggc
     5341 gttgacgtcg atatcctcgg ccgagaccgc ctcctccgcc cgctcgacga gcgcccgtaa
     5401 gtcgcccatc ccgagcagcc gggagatgaa cccgtccggg tcgaaccgtt cgagatcctc
     5461 gatcgtctcg ccgctcccga tgaagacgat gccgctctgc gtctcggcaa cggcggagag
     5521 tgcaccgccg cctttcgcgg tgccgtccat cttggtgacg aggacgccgt cgatcccgat
     5581 cgccgcgtgg aacccgcggg cctgctcgct cgcctgctgg ccgagcgccg cgtcgatcac
     5641 gagccagcgg tggtcggcac gggagatctc gttgatgttg acgatctcct cgatcaggtt
     5701 ctcctcaagg gcgtgccgcc cctgggtatc gatgatgatc acttcatact gcttgcggaa
     5761 tttgggcagc ccctcccgga cgatccggag ggcgtcggat tcgttcgggt ccccaaaaca
     5821 cgcaacgccg atccggtcgc agagcgtctt taactggtcg tacgcacccg gacggaaggt
     5881 atcggcacag atcaccccga cccgaagccc tttgcgctgg aagtagcggg cgagcttcgc
     5941 ggtcgtcgta gtcttgccgc tcccctgcag gcccgccata aggatggtct ggggcccgag
     6001 cgtcacttcg cccggcgtcc ccatcatccg gacgagttcc tggtagacga tccggaggac
     6061 gtgctcccgg acacccatcc ctttgggggg ctcctcctcg agggcacgct
//
LOCUS       DAJB01000260            8133 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_56496, whole genome shotgun sequence.
ACCESSION   DAJB01000260 DAJB01000000
VERSION     DAJB01000260.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_56496"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1352 a         2689 c         2512 g         1578 t
ORIGIN      
        1 gagagcccgg aacgtctttt cgctcccacg cagttcggcc tccgcccggt gacgctggag
       61 ggcaatcgaa gcaaggctcc ggaagacctc gacggcccgg aaattgacct ctccgccttc
      121 ccgtcgggtg agtatcacgg ccgacccgaa gatttcgcct ttccaggtga acgggatgca
      181 gtacattatc ccgaggccgg caatagcctc gaatttacgg cagatcccct gagatatctg
      241 cccgagggcg acttcctgca gccctccttt cacctcctcg atctcgccgc ggatcatgat
      301 tgcccggata tgcgggggaa tttcgagcga gatcccggtg aggtcctccc cgaaggtctc
      361 gataatatcc ggcaggaacg gctcgatgcc gaagaacgcc cgcgggctca gggtgcccgc
      421 ccggatatcg gcggcgctca cgatgacgat ggaaccgggc gcgagttcat ggagataggc
      481 gccgattgcg gagaagacgt cggcctcgtc cggcatcccg gcaagttccg tcgccgcccg
      541 ggagaggaag gccagatcgt ctgatgtccg ccgcacatct ccttttgcgg cctcttggcg
      601 ggtgacgtcg cggaagcgga cgatccagtc accggtctgc ctcacccgcc ttgaggagaa
      661 gacgtaccac cactcaccgg cggccgagga taccctgacc gtgatgccct cggactcggt
      721 gcggttttgc agcagcgaga ggatctgctc cctttggtcc ccatccgctt ccgggaggat
      781 ccggtcggcg acgatgcggg ccgcatcctt attgcgggcg tcatcggcgg agatcccggc
      841 gaggcgctcc atcgaagggg tgatggtccg gacgttcatg tcggcatcgg cgaggatgac
      901 ggcatcctcc atggcatcca gtatctccag tatctcgggt cgcacccctg tgtccggttc
      961 agtggtctgc atacggccca gccccgtccg gcaggatcgc catcgacagg gagatcctcg
     1021 tgcagggttc tccacgggga gaacgtcatg gagagtatat atagtctgtc tgccggcccg
     1081 tccgggcagc cagcccgccc tttttgtgcg ccgggggatc tcgctacggc aggagttttg
     1141 ggccctgtca caacaggcat gtgcgggaac ggctggaata acgggaggac ttgaaagtgg
     1201 agggaatata acccaaaaat cgaaatgcga gggaggcgat tcgaacgccc gaacaccttc
     1261 gtgacaaggc cctcaacctt gcgcctttga cctggctcgg caaccctcgc gctgttctct
     1321 atatgcaacg cactgcaaca taaaatatct tgctctccag gacccggttt tgtgcggacg
     1381 caaaggcctt gagcccccgc gaagtggtct gtctctggac atacccgcgc tccggcgccc
     1441 cgctaccgcc ttcggtgtat atttatgcgc tccgctccaa taatcccgca tgtgggctga
     1501 catctcacgg gagttcgccg attcaccgtc ccagggccgg gtagtccggt tcatgctgga
     1561 gaacgggttc ggcatcaatt cggaggggcg catcgtctgc aacggcatcg agatcccggc
     1621 cacgcacatc ggcagggcga tcgagaccga tcgcagggtc gtggacgcca cagcccgccg
     1681 gatcctcgag atccctgagc tgcgggaggt cttcatccgg atgcgcgccg cgcccgacct
     1741 ctcccgggtc gccgaggctc tcggcctctc ggtcatcacg ctcttcccca aggacgcgca
     1801 cgagaagggg atcgtcggcg cggcggtcaa agtcctcgtc gaccacgacc tctccatccg
     1861 ccagatcttc gtcaccgacc cctacctcgc agaggagccc aggctcgtta tgatcgtgga
     1921 cgaggcacgg gtcccnnatc gtgatgatac gggtcccctt caccaggttg tccgcgtggg
     1981 cgacgatctt ctcctcaagc gtccggggca tacaatcccg cggcacgagg ccgaggagcg
     2041 agcactcgtc agccgtcagc ccggcgccga tgtgccgctc gacgatggcg acgatcgctt
     2101 tgtcaagacc gaacgaccgg cagatcgcgc cgcccgcctc ggcatggtga aggtcgtgcg
     2161 tcactccccg gccgatatcg tggaggaggg cccctgcctc gaccagggga tcggaggcgt
     2221 aggtgagtgc aaggtcacgc accgcccggc agtgggcgat gaccccctcg gtgcacccgg
     2281 cgcgcctgag aagactgatg cagtccgact cacgcactcc cgaggctctc cttgatcttg
     2341 tcgacccttt tccgaagggc ggcggcgatg agctcgttat cgaagtgctc gagcatcacc
     2401 tcgcagttcg gacactcgaa gttccagttc gccgcatccg tgaaggtgta gacgacgctg
     2461 cagttcttgc acatataaaa gtcgttcttc tcctcgtagt tgagccgggc gtcgaggtga
     2521 tcgagcgcat cccggatcac ctcctcaagg acgtcgtgga cgcggtccac gcggaggtgc
     2581 cagaggtagg tgagccagcc ggtctcggtg ttcttcagcc gccggtactc agcgaggcgt
     2641 ttctcataga gggtgtagag ggtgtgacgg acggtgttga ggttggcccc ggtcatctcg
     2701 gcaagctcct cgtcactgtg ctcccctcct tcggggaacc gcctgagaag ttcgactccg
     2761 tcctctccga tcatgcgcag gatgtaggcg tggattgccg gatcgtttaa taattcagtg
     2821 acggttacca tgctcgaatc tctcccatag ttggattatc gtataatagt atatgttcca
     2881 gcatcgatgc ccaccatact ctgttctcaa tctttaattc ccttttcccg tgcccggggg
     2941 cagggcagcc cgtcaaggca gagaagcggg cgttctcggg gttcattccc cgatcaactt
     3001 ccggactgcc gcggtgttcc tctcgaggtc catagatgcc gctgcctccg tctctccgca
     3061 cccgaagacg ctcacgatgg cgtggccctc ctcgaactcg gtgccgggcc agggaatgtc
     3121 ggcgatccgg ggcgcaagcg ccgcgaggtc gacatccagc cgcatatccc gctccgcaaa
     3181 aaggatccgc cggaccgcga cctgcctggc cgcaggcatc gccgccggga tcaccccgcg
     3241 gcaggcatcc atgtgcatgg aaaagacgct cttttgggtc gccatctcga ccgtatccac
     3301 ggtggcctgg aaccgggggt tgatctcgat cgcccagggc ttctcgcccg ccatgaagtc
     3361 gatcccgacc gagcccacgc acccgctcgc cgcagcgatc cgctccgctg ccgcgatcat
     3421 ctccgcggca agcgggtggg agaacggggt gaccgacccg gcaaacccgt gcgcgctctc
     3481 gccctcgccc cgcaggatct gccggttgac ggcgacggcc cgcgcgcgcc ggccgtcggc
     3541 aacgcaggag acgcttgaag ggatcccgtc gacgagcgcc tgggcgacgt agggcacgtc
     3601 cggccaggtc tcctcccacc ggcgaagttc ctcccccgtc ttcacgacgg cgttcctcca
     3661 tccgcccgct ccgcggcgcg gcttgaccat caccggaaag tcttcgctcc cggcgagcgg
     3721 cgggaccggc acctccagcc cctcgaagaa ccgctggatc tccagcttgt cgaggaaccg
     3781 ctcaaccctg tggggcggcg tgccgcagag cgggatcgtc gtcccgatcg tctcggcgcc
     3841 cgaggcgacg accagcgcgt cgaccgggtg gcgggcggcg agttcggtaa tcttctccgg
     3901 gagtttctcg agttcgtcga acccgagaga gtcctctgta taccaggcga ggtcctggtc
     3961 gcagaagtgg tcgacggcgt agaccgtata gccggcccgg tgggccgact gcgccacgtg
     4021 ccgtgtcgcg aacccggcaa tcaggacccg tcccttcact gctccaccgt cttctttccc
     4081 accttcgtcg gttcgatccg gatcttcgcg tccgggtact cccggttgag ctccttccct
     4141 tcgaagaggt ggtcgaggaa gagggcgagg cttgagatct cggagtgggg ctgggtggtc
     4201 accgagacgt tgtagtcggc aaggccgtag atgtcgcccg gcaccttctc tgcccccacc
     4261 acgacgagca cccgctcctg gccccggatc tcgtcgatga cgtcggtcat ccggagcccg
     4321 tacatcgtga ggtgggcgac cttgccgccg ccggccttcc agtcctggat gcaccgccgc
     4381 cacttcacgt cgttctccac aaaaaagtct cctccccacc gggaggccac gtccttgatg
     4441 ctcgtggcca cgccgtgatc gtctgcggca agatacatcc cccgggcccc gagcgctcgc
     4501 gccgtgagcc cgacatgggt cgttacccgc tggtcgcgtt cgggccggtg gcctatccgg
     4561 agtaccgctg cggtgggcat atcgctcact cctctcgcct ctttgccctg acgacgccgt
     4621 tctcgatgac gaacggcata tcattcgtat accggcactg gcactcgatc agcgactcct
     4681 ggatggagag gtggcgcacc gtctccccgg atcgccggat caggagatag cgctcgaggc
     4741 gctcaagcga tgccatgacc gcaccatgtt caaaaccggt ggctgctgcc agatccccgg
     4801 cggtaatgcc atcggtttcg ggaatccggt ggtatatggt ccagtccaga tcttcctcac
     4861 gcacacctat tcttttttcc ggacagaccg atatagttat tcatgcaccg cccgcaccgt
     4921 tccggctctg cgggccgcta tcacggcggt tctttagccg gtgcagggca gcggaaggta
     4981 tggtgggaac tgagtatggt cgattatgca gcactggcag atcttgccga tgggctcttt
     5041 gaggcgagcg acgatgacga tgaacttctc gcagcgatgc tggatccgct tgacggagag
     5101 atcctggggg cgctgctcgc ctccgacctc ctcaacgcct accaggtctt ctactacttt
     5161 ttccgggaga cgccggacga gctgacgaag gagcggctgc agctccacgc ggcctcagat
     5221 ctcgcccggg ggatcgtcat cgacgaggtc gatatctacg atgtcatctt ctctctggag
     5281 ggcggggagc ccgtcctcct tatcaccgac ggcgaggcga cgctggcacg gttcgaagga
     5341 gagcaggcat acacggatct ggttcggtat ctggacgagt ccctttagac gggcgatcta
     5401 gcagaaaccc tgcgggtttc ttcaactcct gccgtgccgc cagtcgtttc cggcggtgat
     5461 cagccgctcg tccaccctcg cccgcacgat cggggggagg tccccccacc ggataaccgc
     5521 atcgccgatg acggcaagca cgccccggat acctgtcccc gggagggcgt cgaggaccgg
     5581 ggtgtcttcc ggtgtgatcc ggttgcagat cgccgtcgcc caggcgtccg cggcggcgac
     5641 gtcgggggag aggaccgtca cggcgtcggc ggtgccgaaa ctgatggacg ggcccaccgt
     5701 cgccgatgag gtgcagatcc cgaggatctc ttcctgtggc ggcaccagga acgcgatacg
     5761 cccgctcaag ggcgatgcgc ctgcatagat gcctatctta acctcgcggt cgcttacaag
     5821 ggcaatatcc ccaccgttgt cgacgagggc aaacgtcgcg ccctcctcga ccatcgcctc
     5881 cacccctgcc caggctatgg cgcccgccac cgccgccatg ggcccgaccc cggcaactgc
     5941 cgcggcccgg gccatgcgat cgggggtcct ctccgggtcc ttcggagcat acggctcaag
     6001 cgttgccgaa aaataggagt ccagggcgat ctgccgctcg acctcctgcc gggcggccat
     6061 catgccggat tttgccgccg cgatctcttt gggatcgtcg gcgaggattg tcgtaatcgt
     6121 ctgccggaac tcgaagtgct cccggatcat ctctgttgcg agagggcgcc atgagggcac
     6181 gcctggatgc acttgccaca gaggacgcac cgctctgaga tcacgcgcag acgccactcc
     6241 tcgtcgaagg agaagacttc ctgggggcag acgctgatgc aggctccgca gtcgacgcac
     6301 tccgcctcgt cgagagcgat ggcattctcc atcacgcgga ccgtgaccgc catctcctcg
     6361 aggcggcgcc ggaccaggtc cgcatcctta tcggggacgt cgatcagcac ctcccctgcc
     6421 atcgaatcga tgttggcctt ctcgacgttg acgaggacgc cggtctcctt caccagccgg
     6481 gcgatcaccg gttcccgtcc tgggtcgtgc tgtcccttcc gggagaacgc cagcagaagt
     6541 ttcatcgccg ccgcctcccc ccgaagagtt tcccgagatc gatcgccgag aggatgccga
     6601 ccacccggtt cgtcgtatcc accaccggga gcgcgctgat gttgttttgc tccagttttc
     6661 gggcggcgac atccaccggc tcgtcggggg tcgtcttgat caccttcctg gtcatgatgt
     6721 ccttgacctg ccgcagtttc ccgtccgtca ccactgcctt cgagacgtca taggtggtga
     6781 tgatcccgac aagcgtgccg ttgccgtcca ggaccgggag atggttcgtc tcgtccttga
     6841 ggagccgctt tgccgccacc cggatctcct cgtcttccgt gatgctgatc acctgccggt
     6901 tcatgatgtc gcggacgcgg gggctgacgg ccgtctctcg catgggtttt gcacgctttg
     6961 ccgggtcgat ccgccgggtg gggagcgcaa gttccatctt ccctctctct acccagtctt
     7021 tgagttccgt cgccaccgcc cgcgcccgcc ggtagctgga gagcgacgac gtcctgaccg
     7081 tttcctcgcc gatctcgacg gacccgctcc tgaggtcggc gtaactgacc gtcgcaaccg
     7141 agggccggtc acgccgggga gtgccgtaat cgatgatatc ggtccggata tcctcatccc
     7201 gcaccgcggt gctccggacg atcccggtat cgagcaccgg tatcgggacg ccgatgccga
     7261 cgtacagcgt cacgccgtag ccgttcatca ccgcggcgcg caggaactcc tgccgcatct
     7321 tcttcatgtc cccggtcacc atcagcgtcc caaagccgcc gcctgccgag tgctgggtgc
     7381 cctcgccgat gaccattccc tcgccgccgg caaggaagat cgggacgccg ctcccgatga
     7441 cccggaagcc ggggtcgttg gcgagcggcg agagggtccc tgcgccggag taggagacgt
     7501 tgccgcaccg cgggaggagg gtgcccatgt aggtgtggag cgtccggtcg gtggtgtttg
     7561 ccgccgcgtt gtagcgctgg taggagttcc tcgggttcac catgatcgcc tggttcatgt
     7621 cctcaagcag cagctccgtg gtgatgctcc ggcgcgggta gcagtcggtc ccgtgggagg
     7681 ttgcccgcag ttcgacggta ccccccgcaa cgagttcttc gaggacgtgg gcgccgccgt
     7741 agaggcggtc cttggattcg gcctcctggg ttgcgcccag ataggcgtcg accgccgcaa
     7801 tacctccgta tgcttccacg tcgttgagcc agacgcgttc catcctgatg ggggggtcgg
     7861 cgtgcccgaa gttgaagaat gcaccggagg agcacattgc cccgaacgtc ccggtcgtga
     7921 cgacgtcgac ctccgccagt gccccttcct ctcctaactc gtcgacaatg cccggcatct
     7981 cctcggcggt aacaactcgt gcagtaccgt cgcgtatccg ggcattgatg aggtcaaggg
     8041 acttgtccat gaacgtatat tcattttgga atatttatag gtgttgatta ttactttagg
     8101 taatattgag ccggggtatg tggccatccc ggc
//
LOCUS       DAJB01000261            8158 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_84008, whole genome shotgun sequence.
ACCESSION   DAJB01000261 DAJB01000000
VERSION     DAJB01000261.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_84008"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1339 a         2606 c         2541 g         1672 t
ORIGIN      
        1 acgcaccgga catcttcgcg gatctcccac tgggcggcaa gcgtctctga ggtgaagatg
       61 aaccggtcga cccagaagaa gatcaggccg ccgatgaggt tggcgacgat ggtggcggcc
      121 agtgctccga ggcccgcgag caggtagagg acgccggcaa ggagcggcgt cgagagctgc
      181 caccggacga gataaaggcc gtagcgttta aaatttattt ccacctataa aattcgccgg
      241 cccccctcat aaagccgggg ccggcacccg gcatacctcc cccttgagca ccgccggaag
      301 ggagggattg cctgcaccgg gcgcaccggc accgtcagga gagcaccagg atccggcggg
      361 tcaggctctt gtgcacccgc tgctcgtgct cctgctggac ggtgaagtgg cgggcggcga
      421 tcgtcgtgat gtcgcggtgc gtgacgacga ccgcgcgcct tccgggccgg aggatgcgcc
      481 ggatctccgc aagcgatccg tcgtagagcc ggtccatgct ctcggcgtgg atccggaccg
      541 actggccgta ggggagatcc gtcacgaccg cgtcgagcgc cccgtcgcag accgggaccg
      601 ccgtggcgtc ggcgatcatc acgtccgacc ccgggaggtt ctgccggtag ccggcgacca
      661 tcgcggggtc gaagtcgctc ccgagggtcc ggacgccgat ctcttgggcc tcaaggagga
      721 tcccgccggt gccgcagaag gggtcgaaga ccacctcgcc gggccggacc agggagatgt
      781 tgacgagcgc ccggcccatc cggggcatca tgacgccggg gtggaagaac ggccggcgca
      841 tggggttccg ggcatcgaat gccccccggt cgatccgtag tacgacgcgg ccgaggtagc
      901 agcggtcctc cgagattacg gcgcggtact cttcgattgg ctcacggagc gagaccgggc
      961 cggcgatcag ggtcccgatc agccgttcca gttcgaggtg cgcggcatcc atccggcttc
     1021 cctggatcgt ctttaccctg cccgcgaacg gcttatcggt cgttatggcg aggtcgcgaa
     1081 ggaggcccgc gaacgcctcc cgcgtgggct cgcactcccc gaggtactcc atcaccacgt
     1141 gcgtcagggc aaggcgtccg gcggcctccg ggtccgggca ctcggcaacg gcgacctggg
     1201 cccgcctatc gaggacacgg cccacgcact ccagttcaga gaccgggaga tcggggtgct
     1261 cgccggagag ttcgaagagc agtttcatcc tcagggtatt ggggtgggag ggggataaag
     1321 cggggggatg ggggaagcag aggtcttaac atcggtagcc gaaagaccgg ttgggatggc
     1381 ccgactattg ccgttgcagt tgggcgcaat gtgaaagccc cgcagtgcgt gagctcggac
     1441 catgaggttt aatccgctgg cacggatttc gaggggatat cgccacgcaa tgcccccgcc
     1501 ccccccgggg gcgatatccc cacggtccag tgtacggggc tttttcatcg cccctgctgt
     1561 tcgggttgcc ttaactcgcc agtatccaca ttcgggagtc gtaacacaaa aaagataacg
     1621 aaaaaccgaa ccgccgaagc ggggcccccc tcacttcacc gactctttca ccttgtcgac
     1681 gacgtcctga aagaagcctt tgcccttctt agagcccttg gaggagggtt tcttcccctc
     1741 catctcgagc aggcgcatgt agagctcgcg ctcctcctct tttaagtgtt tgggcacgac
     1801 gatgcgcacc cgcaccagca tgtcgccggg tttggtctgc caccgcacgc ccttgcccga
     1861 tatcttcaac ccggtgttgt actgcacgcc ggccgggata tcgaggacga ccgtgcgccc
     1921 gtcgatcgtc tttacctcaa cctcggaacc caggattgcc tgaaccggac tgatatcgac
     1981 ggtcgtctcc aggtcgtcgc ctcgccgggt gaaggtcctg tgcggcgcga cgctgatctc
     2041 gatgtagaga tcccccgccg gcgcaccgta ctccccggcg tcgccgtatc cttccatcct
     2101 gaggcgcatg cccgtatcga cgcccggcgg gatccggacc ttcacggtct gccggccgcg
     2161 ccggtgcccg ctgccgccgc agaccttgca ccgggactcg gggatcttcc cccggccgcc
     2221 gcaggcggtg cagggggtca tcctgacgaa attcccgaag atagactggc tcatctgccg
     2281 catctggccg ctgccgccgc aggtcgggca ggtggtggac ttctttgtct cgctccccga
     2341 cccgtcgcat gcagggcagg gctcgacatg gtcgaggctg acctctttct cggtcccgaa
     2401 cgccgcttcc tcaagcgtca cccgtatctt catcaggaga tcggccccgg ggcgcgggcc
     2461 ggaactctgc ctgcccccgc cgccaccgaa gaaagtctcg aagatatctc caaagccgga
     2521 gaaatccgcg ttgaacccac cggtgggtcc tcccgcgccg gagtacgaac cacgggacgc
     2581 attgctgtag gcgtcatgcc ccagctggtc gtactgggct cgcttctggg cgtcggagag
     2641 gacgctgtag gcctcgttga tcttcttgaa cctctcctcg gcccccggct ccttgcagac
     2701 atcagggtgg tattttcgtg ccaggtcccg gtaggctctc ctgatctcct tatcgtcggc
     2761 gtcacgcgag acgccgaggg tatcatagta gctgtccgga cccatcgacc tcactctttg
     2821 acttcgtaat cggcatcaac gacagtatca tccttcttgg cagctccctc tgtcttctgc
     2881 tgctgggcgg cctgcgcctg ctgatacatc tttgtcgtga ccgcatatac cgcttcggtc
     2941 agggtttcca tcttctgctt gatggcatcg aggtcgtcgc cttcgagcac cttcttgagg
     3001 tcggcgatcg cgctctcgat cttctcgcgg tccgcggcat cgatcgactc tttcgcgtcc
     3061 ttcaaggccc gctcggcggt gaagatcgcg gtatcggcgg tgtttcggag atcgatctcc
     3121 tcgcgcttct tctggtcctc ttccgcgaac ttctttgcgt cgctcatcat ccgctcgatc
     3181 tcggcctccg agggtcggga gtcctgcggc ttgatggtga tcgactgctc gttcccggtg
     3241 ccgaggtcct ttgccgagac atggacgatg ccgtttgcgt cgatatcgaa cgtgacctcg
     3301 atctgcggga tcccccgggg tgcgggcggg atcccggtga gctggaaccg gcccagggtg
     3361 aagttgtcct ttgcaagcgt ccgctcgccc tgcatcacgt ggatctcgac cgaggtctgg
     3421 ccgtcggcgg cggtcgagaa gacctggctc ttcctcgtgg ggatggtggt gttgcggtcg
     3481 atcagtttcg tcgcgatgcc gccgagcgtc tcgatgccga gcgagagcgg cgtcacgtcg
     3541 aggagcagga catccttggt ctcgcccgtc agcacgccgg cctggatggc ggcgccgatg
     3601 gcgacacact cgtcggggtt gatccccttg tcgggctcct tcttgaggat cttcttcacg
     3661 gtctcctgca cgagcgggat acgggtcgac ccgccgacga ggaggacgtg gtcgataccg
     3721 tcagggccca gtttggcgtc ggcgagcgcc tgcttcaccg gcccgagggt cgagtcgacg
     3781 aggtcgccga tcagctgctc gaacttcgca cgggtaaggt cgaagtccag gaacttggga
     3841 ccgctctccg tcgtggtgat gtaggggagg ttgatgttgg tcttctggac ggtggagagc
     3901 tcaactttcg cgttctcggc cgcatcgcgc agccgctgca tggcgaccgg gtccttccgg
     3961 aggtcgccgc cctccttctt cttgaactca tcgaccagat agtcgacgac cagtttgtcg
     4021 aagtcgtcgc cgccgaggcg gttgttgccc gcggtcgact taacctcgaa gacgccgtcg
     4081 ccgagctgga ggatcgagac atcgaacgtg ccgccgccga ggtcgtagac gagcaccgtg
     4141 gactcgcctt ccctgtcgat gccgtaggcg agggcgcttg cagtcggttc gttgatgatc
     4201 cggagcactt cgaggccggc gatggcccct gcgtccttcg tcgcctggcg ctgggcatcg
     4261 ttgaaatagg cgggcaccgt gatgacggct ttcgcgatct tctcgcccag gtactcctcg
     4321 gcgtccagtt tcatcttctg gaggatcatc gcggagatct cttgcggcgt ataccccttg
     4381 ccctcgatcg tgatcttctc gctcgtgccc atcttccgct tgatcgactg gatggtgcgg
     4441 ttcgggttgg tgatcgcctg ccgctttgcg acggtgccga ccagccgctc gccctccttg
     4501 gtgaacgcta cgacggaggg ggtggtcctg ccgccttcgg cgttggcgat gaccgtcgcc
     4561 cgcccgcctt ctatgattgc catgcacgag ttggttgtcc caagatcgat accaagaact
     4621 ttttccgaaa ccatctgttt attcctccaa tcctttagat actacgactt ttgcgcatct
     4681 gataacttta tcctgcatac agtatccgcg aacgacctcg tcgatcacgg tgccctcctc
     4741 tgcgtccgaa ggaacgtagg cgatcgcctc gtgccgctcc gggtcgaaag gaaggttccg
     4801 gcattccatg agcctgatgc cgtgccgctc aaggatggtc gcaaagagtt tcctgatctg
     4861 ctccatccct tccgtgaggg ctgcaccgtt ggccttctcc gcccgctcga agttgtccac
     4921 gacctcgagc aactccaccg caaacccctc gatggcgaat gttgtacgcg cgtcgagttc
     4981 ccgggccatc cttctgcggt agttgtcgaa gtccgctgca aggcgcaggt gacggttgtt
     5041 taagtcgtca tatgccttct gcaactcttc aagcgcggga gatggtgcat ccgccggttc
     5101 tgcgagattc tgctggctct cgtttggttc ggatgtatcc tccttcatac gcttaacctg
     5161 cttcaaatga tgttaatgta gtagttcgat tatttattgt aatgtggttc tatatattac
     5221 taactgttgc tgcagacatc cggctccttt aacgggccaa atcgccgatt gggagcccat
     5281 ccgtcagtat tcccccgtcg atatgcggat ccggaggagg gggacggcct tcctcgccgg
     5341 ccgtcggggt gctctccagg gcatccctcc ctgctttccg tggacaaaca cgcccgcttt
     5401 cgcgggcggc gccaaatata tatccctcta tcccgctcat gtgtacccat gaagtgggca
     5461 ctgctgtctg tctgggataa agcaggcatc gtcgatcttg caaaggtgct cgtcgagcac
     5521 gattaccggc tcctgagttc gggcggcacc ggggccgcgc tcaagcaggc gggaattccg
     5581 ttcaccgacg tctccacgca caccggtttt cccgagatga tgcacgggcg ggtaaagacg
     5641 ctccacccga agatccacgg gggcatcctc gggcgccgcg gcatcgacga cgccgtgatg
     5701 caggagcacg gcatcgagcc gatcgacctt gtggtggtga acctctaccc cttcgaggag
     5761 atgagcgcga cggggctctc ccttgaggag atgatcgagt acatcgatat cggcggcccg
     5821 gcgatggtgc gggcggcggc gaagaaccat aagtacgtcg cggtcgtggt ggagagcgcc
     5881 gactacggga cggcggcgga agccgtccgg cggggcggct ttgcgcccga agagcggctg
     5941 aacctcgctg caaaggcgtt tgcacggacc gcggcttacg atgccgcgat cacgaactac
     6001 ctcaccggca tcgaccggcc gttccccgag gtcctcacgg tccagttccg gaacgggaga
     6061 tcgctccggt acggtgagaa cccgcaccag gcggccgccg tttacggcga taccggcatc
     6121 gccgcagagc agccgctcca ggggaagtcg atgtcctaca acaactacct cgacgtccac
     6181 gcggcggtcg gcctgctccg ggagttcgac gactgcgccg tggtcatcgt caagcacaac
     6241 aacccctgcg gcgtggcgac gggcgacgcc ccccttccga cctacatcgc cgcccgggat
     6301 gtcgacccgg tctcggccta cggctcgatc gtcgccctga accgcgaggt gacggaggac
     6361 gtcgcccggg agtttgtcgg gacgttcgtc gaagtcgtgg tcgccccgtc ctacaccccg
     6421 ggagcgcttg agatcatgaa ggcaaaggag aacatgcggg tgctccggct gccgaagccc
     6481 gtggtgcagg acgagatccg gaccatcgac ggcggcgtcc tgatccagcg cacggagccc
     6541 tgccgcgagg actggcgggt cgtgagcgag cgggagcccg acgcccacga gatgcgggcg
     6601 atgcaacttg ccctgaaggt ctgcaagcac acgaagagca acgccatcat atttgccgac
     6661 gagaaagcgg tcatcggtat cggtgccggg cagatgaacc gggtcgagtc cgcagagatc
     6721 gcggtcagga aggccaggaa gccccttgcc ggctcggcgg tcgcctcgga cgccttcctg
     6781 ccgttccccg acaccctgga ggtcgcggcg gcggcgggcg cgacggcgct cgtccagccg
     6841 ggcggctcca tccgcgatgc ggaggtcatc gaggcggcga accggctcaa catcgcgatg
     6901 gtctttaccg gcgtgcggca cttccggcac tgagaggccg gccggagatc tcttttttcg
     6961 taagtccgca ttccagccgg gtctgccatg aatcttcgag cactacaggg ataagtgccg
     7021 cgttatggtc gtcggcacgg gagttcgtcc ccgtagtctc cggttctatg agcgctgcgg
     7081 gtttgccgta tctcatcgcg tcgagaactt ctttgtcgat aattacgacc acccgatctt
     7141 cgactgcggc gaagagctcg tcgagatggt ctacctgaga aaggagttgt gacaatccgg
     7201 gagttctgcc ggatcccgat ttttgagccg gaaggccgcc gacacggcga accggctcaa
     7261 gaacgcgacg gtcttttccg ggtgctacct ctatgacagc cctgtgagat cgcgtccaaa
     7321 ctgttatatc ccccgccccc tctccacggc cgaaggtggt agtgatatgg actacggtaa
     7381 tcttgtctcc ggctcggtcg gttacacgaa ggaggctctc tggggcgaat ggggacggtg
     7441 ggtgctcctg gtcatcctct cgctcatcca gacgttcacg ctctttttag tcccgctttt
     7501 taacggctac atcgtccggg tgctctccgg caggacgccc gcccccgacg tcaacgaatg
     7561 gggcaggctc ttcgtcgacg ggtggaagtg gaacatcatc aacctgatct acatgatccc
     7621 ggtgatcctg atcctggcct acttcggggg ggttgcagtc atctccacca tagcggcgta
     7681 tggggtggac aaccccgacg tatgggcccc ggccgttgcc gccgcgatct ccggcatcct
     7741 gattgcggtc gtcgtctggg tcatcatatc gttcatctcc ctcttcgcgg tcgtgcggtt
     7801 cgcccacacg gacagcctcg gccaggcgtt caacttcggt gcgatctttt cgcatatcgg
     7861 acggatcggg tggggttcct ggatcgtcgc gatcatcgtc ctcctcctca tcggtctcgt
     7921 gtatgcggtc gtgctcggcc tcatcgcctc gatcccgatc ctcggctgga tcatcaacct
     7981 cttcctcggg gtggcgttcg gcatcttcca cgcccactac ctcgcccggg cctacgagag
     8041 cgctccggca ccggcgtaaa accccacgcc ccttttctgg tgcccctctt cgggcacaca
     8101 cccttcaccg ttatcaagac cgggtgtttg tatcctgaat agatagaatt tacacctt
//
LOCUS       DAJB01000262           11636 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_137427, whole genome shotgun sequence.
ACCESSION   DAJB01000262 DAJB01000000
VERSION     DAJB01000262.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11636)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_137427"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         2398 a         3380 c         3487 g         2371 t
ORIGIN      
        1 gtcgtggact atgcggtgag ccggcccgac gtcgacgaag accggattgc actctggggg
       61 atctcgctcg gcggctacct cgccccccgc ggtgccgcat acgagctccg gatcacggct
      121 ctggtcgcgg acgcaggcgc ctacgatgtc ggactaactc tcctcgcgaa cctgcaggaa
      181 ggcgggggag cgaacctgac agaaattgaa ctgcaggagt ggctgcagac agatccggtt
      241 gagttcaacg atgccctccg cgatgccatg gcacacgata ccggcacccg ctggctgaac
      301 gagaacggga tgttcgtctt tgatgccggc tcgccggcgc agttctgggc gaaatggatg
      361 gatttttcac cttccttatg tgaatctgtg aggtcactat cgggacgatg cggatcatct
      421 ctgtatccgg gggatggacc aggtcgtccc ggtcctcggg ttcctcgagg tcttcatctg
      481 gatcattgcc atcggcaaga tctttcaaaa tttcacgagc ccgctcaact gcgaccggtt
      541 ttgatgatgg acactttgtc gcgtaggtag gagcccgcat tggcctccgg gaacgtctcc
      601 aggtgcaatt cacccccggc ttccttacaa cgaccaaaac tcagaaatac tggtggcaat
      661 acactggaat actgttaacc tagtggccct gattataggg aggttgtgga tatgaagaaa
      721 ggatttgtcg cagatatcga aaaggaaacc ttaaagaaca ccgacttccg cagagtcctc
      781 tatacgggga agttcagcca gctcgtgctg atgtgcctta agcccggcga ggagatcggc
      841 gaggaagtgc atgatgacgt cgaccagttc ttccggttcg aggaaggcga aggagccgtc
      901 gtgatcgatg atgtaacgca cgccgtcaag gacggcagcg ctgtggtcgt gccgagcagc
      961 gcgaagcaca acgtgctgaa cacgtcaaag accgccaatc ttaaactcta caccatctac
     1021 tccccgccgg agcaccagga caaggtcgtc cggaagaccc gcgaggaggc catggcccgg
     1081 gaagagcact acgacggaaa gccgaccgag taactctccc tcgaactgct aagaaccaat
     1141 ttttcatttt tcaacagttg ttaaaagtta tcgtgccggt atgcatgaag tggggcgtct
     1201 cttccgtttc acagcctgct gtcgggcgaa tttctttgct cccggtggtc gctgcagaac
     1261 gactgcctcc cgtccgccgc tcgcatcacc ggctcggagc tcgggcatgc atcaaggttg
     1321 ttcgacccga tgccgacgga tgctcggtgg caccccatct gcaatcatgc agtattcccc
     1381 gcgtcattca ctctacccgt tccagaacca gagcaccgag gtatgccatg gtctggagga
     1441 tctctttatt ctccggcgtc agcatctcat ccgggttcct gaacgcgaaa gctaaaaccc
     1501 cgaatgtaac acgggatgcc ctcatcggaa cgtaatgccc tacgcccgcc ggaagcgtct
     1561 cagtgcctct gcctgccacc tgaccattct caaacaccca ctgggcaatt gtcctctctt
     1621 ttgacgtgag gggataatcg ggatctccga cgctcataaa tagtcccccc actctgggca
     1681 ttaaaacggc acaggtaccc tcaccgaatt gcttcatatg gcgatagagg gtattgagaa
     1741 gctcctggcg atgggtgaca ttcgccaggt cgcgggagag gacggtgaca gcctcgacct
     1801 ccgcttcgct cctccgtatc tgcgggagca ggttgcggag ccttgtagca aggctgctga
     1861 tgatgaacgc aatgaccaca tatcccacaa aagagatgaa atattcccag tcagcgatgg
     1921 taaacgtgta atagggcacc acaaaagcaa agtcaaagac cagaatgctt gcaatcgcgg
     1981 ccactatcgc tgtctcccga cggaagaaga gcgctgccac caccaccgga atcaactgga
     2041 taataagcag gtttgacgga ctgatgaaac cccgcaggaa ataattcgtg atcgttacca
     2101 gcgcgatgag caccgtgctg atagtaagat tcaggctcac aaggtgcggt aactgtcggc
     2161 gaatggggat ttttactgct gctttcggtt cgtaaaggaa gatatcgatt ccctttgacc
     2221 gaaccatgat ccggtacacg ggagaggaga agatgtccag cccccggggt ttgccgagca
     2281 taatcatggt gacgttgtgc tgccgggcgt accggagcag ctcgccagcc acattctctc
     2341 cgcggtagcg tacgatccgt cccccaaggc cccttgctat ctccattgca tcgttgagcc
     2401 accgccgttc ctggtccgtg agattcagtt cttcctcggt tccgatagag atcacgatcc
     2461 agtccgtgcg gaatctgtcc gccagacggt acgccgcacg aaccatctga tccgccgtcg
     2521 ggccggggcg tatccccaca aggagccgtt ccccggcggg ccagggccct ggaatagccc
     2581 tggcacgcat ctgggtgagc atctgccgat cggtgatctg cgccacatac cggagggcaa
     2641 tttgccggag tgccagcaaa ttttctgtgc tgaagaatcg ctggacggcc tgctcggcca
     2701 tatcctgcac gtagaccttt ccggcacgga ggcggacccg gagttcctca gggggaatat
     2761 ccacaaggcg gacctcatcg gcgtcgtaga agaacgtatc cggcaccgtc tccgtgacgt
     2821 gaatgcccgt gatctgggcg acaatgtcat tcagactctc gatatgctgg atgttgacgg
     2881 tggtgtagac agatattccc gcgtgaagga tctcttcgat ggcctggtag cgtttggtgt
     2941 ggcggctccc cggagcattg gtgtgcgcga gttcgtcgat gaggacgacc tcgggacggc
     3001 gccgcaggac cgcgtcaaga tccagctccc gcagggtcag gccctggtaa tcgacggaga
     3061 tcgaggggag ggcctcgagc ctggcggcga gaacatccgt ctcggctctc ccgtgcgtct
     3121 ccacatgccc gatcacgata tcgagcccgt ccctccggtg ggaaagtgca tcctggagca
     3181 tcgcataggt cttcccgaca ccggcggcgt agcccaggta gaccgtcagc tgcccctctt
     3241 ttgctttctg ttccgcacgt tctgctacta tgagcagatc gtcgggctgg gggcggcctt
     3301 cttcggcacc tctatccgct gtcatcgccc ccccccatcc agacggtcca gtgcacgatt
     3361 gagcgagagg acgttgacat actgccctgt gaacgggatc ggcgggctct ccgcctgggc
     3421 gagaaccagt gcacggactt catcctccgg gagattgcgt gcctccgcca cgacgggaac
     3481 ctgcaccagc gcggcatcca ggctgatatc ggggtcgaga ccgctccctg acgccatgac
     3541 cagatcggat gagatgaagc cgttcactcc agcgtttcgg agaacactca cccgttctgc
     3601 gatctgctcc aggagcaggg ggtttatcgg cgccagattg gagccgccgg aggaagaggc
     3661 gttgtacgcg gtgaccggcg tcgccgaggg gcggctctgg aagtaaaacg gtgcgctgaa
     3721 gttctggccg atcaggctcg agccgatcac cctgccctgg tcgccgatga tcaggctccc
     3781 gtgtgcctgg tacgggaatg ccgtcccggc gatgagcgtt accgccagtg aatagacgaa
     3841 tcccgtgata acgaagagga cgaggaagag gagtagaccg cttttcactg catggcgtat
     3901 atccgtcacg aataagcccc tcctcagata tgcaggattc cgacgatcac gaggtcgatc
     3961 gctttaatgc cgatgaacgg ggcgacgagg cctccgatac cgaagatgat caggttgtag
     4021 gcgaagagcc gcgatgccgg catcggtttg aacttcaccc ccgcgagcgc gattgggatc
     4081 aggagcggga tgaccagagc gttaaagatg accgctgaga ggatagcaga ctccggagtt
     4141 gcgaggccca tgacgttcag cactacgagt tgcgggtaga tcccgaccag agcggccgga
     4201 atgatggcga aatacttggc gatatcgttc gcgatggaga atgtggtgag ggctccgcgg
     4261 gtcatcagga tctgtttgcc gatctcgacg atatccagta gtttggtggg gttgctgtcc
     4321 agatcgatga tgttggctgc ctcccgcgcc ggctgggtcc cgttgttcat ggccaccgcc
     4381 acatcggcct gagcgagggc aggcgcatcg ttggtcccgt cgcccgtcat ggcaaccatg
     4441 tagccctcgt tctgggtctc gcggatcaat cgcagcttgt cgtccggctt cgcttcggca
     4501 aggtaatcgt ccacgcccgc ttcggctgcg atcgcagccg cagtgagcgg attgtcgccc
     4561 gtgatcatga ccgttcgtat tcctatccgg cgaagatcgg caaaccgttc cctgatgccg
     4621 gtcttcagga tatccttgag gaagatcacg ccgagtatcg tctcattgcg ggtcaccacc
     4681 aacggtgtgc ctcctgccga tgcgattccg ttcacttttg ccatcaggtc gagtgggatc
     4741 gcccccccat gctcctgaac catctgcgta atcgcgtcgg cagctccctt cagatatgaa
     4801 gttccgccgc actcggcacc gcttacccgg gtggcggccg agaacgggat gaatctggtc
     4861 tcaggagagc aggccggaat ggtgccggtc ttctcctgaa caagggcaat gacgctcctg
     4921 ccctccgggg tctcatcggc atatgaggag agaatggaag cttgcagcaa ttcgtcgcgg
     4981 gattgccctt ccaccggaac aaactctgtc gcctgacgat ttcccagggt aatcgtcccg
     5041 gtcttgtcga ggagcagaac gttgacatcg cctgccgcct cgatcgctct gccggagagt
     5101 gcaacgatgt tccgctggaa gaggcggtcc attccggcaa tcccaatcgc cgggaggagt
     5161 gcagcgatag ttgtgggtgc caggcaaacg aagagtgcga ccagcaccgt gagggtcaca
     5221 ggagcgcccg aaccggtgaa cgaggcgctg taagcagata ccgggtatag gttgccgacg
     5281 accgccagga agacggcggt cagcgctagc agcaagattt cgagtgcaac ctcgttgggt
     5341 gtcttgcgcc gttctgcacc ctcgaccatg gcgatcatgc ggtcgaggaa ggtctgcccg
     5401 ggttcggtgg tgatccggac gatgatctcg ttcgcgagca ccgtggttcc cccggtcacc
     5461 gcactgcggt cccctcccgc ctcgcgcacc accggtgcgg attctccggt gatggcggct
     5521 tcgttgacca gggcggctcc tttcaccact tccccatcgc ccgggacgat ctcgtttgcc
     5581 ctgacgagga cgagatcgcc cgaccggagg tcggaggagg tgacttcttc ggtcggagca
     5641 tcgaatgcct ccttcctgag gcggtgtgca ggcacactgg cacgggactg ccgtagcgac
     5701 gccgcccgcg cctttccccg cccctcggag agagattccg cgaagttgga gaagattacg
     5761 gtcaaccaga gccagaggga gacgagtccg gtgaataccg tctgttcggg ggttgcagtg
     5821 gcgagaccga cgaaaaaaat cgccgttgta gtgatccccc cgatctccac cgccagcatt
     5881 accggattgc cgatctgctt gcggggatcc agtttgagca cggtgccggc gagcgcttca
     5941 cggataagtc cgggctcaag agcaccgaac cgcggatatc gtttccgggc catcggacta
     6001 ccctcctccg aacaatagca ggtagtcggc aatcggcccc atggcaaaga gagggaagaa
     6061 tgtgagcgcc ccgacgatca ggattaccag gatcgtccag acggtaaatg tcactgagac
     6121 ggtaggcagg gtttcgggtc cccggggagc aaccttcttc tcgaccatcg agccggccag
     6181 ggccagcatt gcgaccgcgg ggacaaaccg cccgatggcc atcgccgcgg cgccggtgag
     6241 tgcgtagaat gcccgggagg catcgaaccc ggcaaatgca ctcccgttgt tgttggacat
     6301 ggaggcgaag gtgtaaatga gttcggagag accgtgcggg ccggggttat gcatcgcctc
     6361 ggtcacgcca ggcaggagca gtgcaactcc tgtgaggacg agggccagca ctcctgggac
     6421 caggacggtg agaacggcca tgcgcatctc ctggacctcg atcttcttgc caaggtactc
     6481 gggagttctc ccaatcatca gcccggcgat aaagacggcg accacaataa aaccgatgaa
     6541 ggtatagaat cccgaaccca ccccgccgaa gacgacttcg ccgagcagga tcaggaacat
     6601 cggcatcatg ccggcgatcg gtgtgaaaga gtcgaacatg gcgttgaccg ctccacagga
     6661 tgtcgcggtg gtggaggtgg cgaagagggc ggtgccgccg agcccgaacc gcacgtcttt
     6721 gccttccatg gagattccgg tcacgccgta ctgccccacg agcggattgc cgcccaattc
     6781 tgcggtatag agcacaccga gggcggagag gtagatcacc agcataacgg tgtagatggc
     6841 ccacccctgc cgcattgacc cggccatccg cccaaagacg aagggcaggg cggtcgggat
     6901 cagcaggatc aggaagatct ctatgaggtt ggtgaacggt gtcgggtttt cgtaggggtg
     6961 ggcggagttg gcgttgaaga acccgccccc gttcgtgccg aactccttga tggcctcctg
     7021 agaggcgacg ggccccatgg cgattgtctg cggtccggcc gggccatacc ccgtggcatg
     7081 cacgtaggga tctaagttct ggatgacccc ctgcgagacg agcaggaggg cgatgatgaa
     7141 tgcaatcggg aggaggatgt agaggacgca ccgggtcatg tccacccaga aattgccgat
     7201 ccgatcagtc gaccggcggg tgatcccccg catcaccgcg atggcgatgc agatgccggt
     7261 ggcggcagaa agaaagttct ggaccgcgaa acctgccatc tgagtcaggt agcttgccac
     7321 cacttctccc gcataaatct ggtagttggt gttggtgacg aaactgaccg ccgtgtggat
     7381 tgccgtgaga aggttgaatg cactgaatcc ctgcgggttc agcggcaggt aaccctgcaa
     7441 caggagaagc gagaagagag cgaggaagcc gatgccgttg aagatgagca tgtcccgcgt
     7501 gtaacccttc cacccctcct ccgtatctgc cgacgtgccg atgaaacgga agagccggat
     7561 ctccagcgtt ccgaggatgc cgtgggagta gtccccggaa aagacatgga ccatatatct
     7621 gctgaagaac actcccgcga cggttgcgac tgcaaggaac gcgatgaaga ggaacccatc
     7681 gaggagtggt tcaggttgtg ccataacgga tcatccggtt tgtatagatc tggaaggata
     7741 attcctacca ttataaaatt aacgtccctg cggagtagtg ctatccagat ccgtcgtccc
     7801 gggcgaggag ggcaatggtg gaataccaga cgggcgccag tggcgatcgg gaagggagcg
     7861 cagcgatccg tatgccgtta taactgttat ttcgaggttc ccgacaaacg tctcgcgtat
     7921 ccggttcttt tctcgttatg ggtatcggag gcgccgaggg tatacagctt tttatcggag
     7981 agcgtgccac gctgtataca tgaggcgcat cgaacacctg gcgtccatcg ccggtgacat
     8041 cggcgcgata ttccggtggc tcggtatcct cgcccttgca cccttcatcg tgatcgtcat
     8101 cttcggggaa tggcagatga tcgtccccat ggctccggta ccgatactct ttttcgtcct
     8161 cggttatctg ttgcaaaggg tccgtccgaa cgaacgccac ccaaagttca gtgtcgcgct
     8221 ggtcggtgtg gcgttcacct ggctctttgc ggcgctcatc ggggcgatcc catttctgct
     8281 cggtgcccag atgtctgtta cggatagcct cttcgagtcg atgtcgggat ggacgaccag
     8341 cggtttctcc gttgttccgg tcatcgacga tctcccccag acacttgtct tctggcggtc
     8401 gtacacccaa tggatcgggg gaatcgggat catcgcattc ggggtcgcga tgcacaaccg
     8461 gtctgccatt gcactctcgc gcctttacca ttcggaaggc aggccggagg agtttatgcc
     8521 gagtgtgatc ggcaccgcac ggcgtgtctg ggtcgtctac ctggcactca cggtcgcatt
     8581 caccgtcttg atcctgttct ccgggattcc gctctgggat tcgctcaacc tcgtgatggt
     8641 tgcgatatcg accggcgggt tctccaacca cgacggcggg gtgctctttt atcagaaccc
     8701 ggtgctggaa ggaatgctca tcccggtcat gattgccggc tctcttccga ttcaggtcgg
     8761 tatcctgctt tattacggca aatttcagtc gttcctcaag agccgggtcc tccacttcat
     8821 cacgatcgcg accctgatag tctccgccct cgtgatcctc aacctgcacc ggaacaacgg
     8881 gatgccgcta tcgcaggcga ttcgcgaggg tgtcttctgc gtcgtctcga gctttaccac
     8941 caccgggttc cagaactccg acatactcct ctgggcaccg gtcgcagttg tcctgctcac
     9001 gatcctgatg ttcatcggga catcgctcgg gagcaccggc ggcggcatta aggtcaaccg
     9061 tctccttctc gcctacagcg cactggtctg gtggttcaag cggctcttcg tgggcaagaa
     9121 tgtgaaaatt ccattccgtc acgagggggt gacgtatccg gaatcggtct cggaggtcga
     9181 gatcgcaaag aacatgctga tcatctttct gtacttctcc acctgcgtcg ctgcgacggt
     9241 gttcgcgctc caggtcacga cgacatcctt taccgtctac gaagttctct ttgatgtagt
     9301 gtcggcactc agcaatgtcg gcttgagtgt cggatacctg acgcctgcct caccactgcc
     9361 cattaagtgg ctcttcacct tcatgatgtg gatcgggcga ttggagtttg tggcagtgat
     9421 cgcgctgttt atcggtctcg tgaaggggtt cgacgccttc gaggttagat gacagtaaac
     9481 gtcgagaagt cgccgggcgt gcatcaaggc tgctccggtt aagcaatatt aacctcccgc
     9541 aatacgggca gaatcctgcc gaatttcgac ccttacataa gcccacaggc agcccgcggt
     9601 gcccgacagg gtatacatcc tcctgtaatt cccctcgctc atatggccgc tacatctcaa
     9661 cgatttttcg ctaatctgga accaagccaa tatgcttcgg ctaatcccag cgccgaaatc
     9721 cggcatccag aaccctcggt aaattcgcaa aataacggta attcggccct atttctgggc
     9781 ggggagacgg tgagtcctca tcacccgggc gccgggcaga cctgcggcgg agtgaagggg
     9841 ccagcttacg ccgccgcctc cccggagaga tgagcctgga cgaaggtgac cgccatatcc
     9901 ccggcccttt cggtgaacat ctggaccgcg atgccgagga ggacagcgac ggcatggcgg
     9961 atgcgcgtgc tgccgaggtc ggcggtgtac ctgagaaaga acgggatgtt gccgattggg
    10021 tttgcgatgg caaaagtgcc acaaagaagg caacgaagaa ggatgcctgt tccatgcagg
    10081 accgtgaatc tagcagcacg atgaacgttt tgaagcttgc cactccgaat ggggagagac
    10141 agacccttca gtcccgctcg atctcgccgc ccgcctcgcg gatcgtcgtc tcgatatgct
    10201 ccagtagatc ggcgtcgtgg gtggcaaagt ccagcatcga aaatcctccc acgaggggga
    10261 tgggcttgaa gatcgcgagc ggcgattttc cgcttttttc cgggttatcg cccggggtga
    10321 tgacggcggt cgttccatcc ccccacacgg cgaggttccc gtagccctgt gcgtcccaga
    10381 tctttttgac gagctcacgt gtgtacagca ttttctgttc ccggaagaaa aggtgtgtga
    10441 agagggcatc aaccttccgg cacggtagtg ccgcaacgta tgcccgggcc gttttcttac
    10501 ctcaccggcg gagcctcggg ctccccgccc tccaccgaag gcgtccgctc tcccgtaaag
    10561 aggtggtcga cacccaggat cgtgttcacc caggcccagt catcgctgaa ctggacgatc
    10621 atcaggacga tgagggtgag cggaacggcg aagatcatcc cggcaacgcc gagagcccat
    10681 ccccagaaga tgaccgagag aatgactacg agggcgggaa tgtcgaagcg cctcgacgcg
    10741 aagtgtgcaa agatcggatt ctccaccagc gcgttcagga ccgcgacggc tgcaatcacc
    10801 gcggccgcgc cccagagccc gaactgcagc caggcaaaga agatcgccgg gagtgccgcg
    10861 acgatcaggc cgatgtaggg gatgtatgca agcagaaacg tcagcactcc ccagagtacg
    10921 gctgcgtgca cccccatgac ccagaggatc gtgccgaaga ggaaaccgtg gacgaggttt
    10981 gcctcggtcc tgacgatgac gaagtcgatc aggaacctgc tcatgcggga gaacccctcg
    11041 acactctcgg atcttttcgt caccctctgt atgcgctcag gcatccgcgg tgcctcaagg
    11101 agcgtgaaaa ttgtcgttac cgctataaag aagaggtaga ggagaaggtc cgcgaggctt
    11161 acggcggacg aagagaggaa tccggcgaac ccctgcaggt tgaccgaaga aggggagaac
    11221 gagcccgtgt cgaccccgta cctgtccagg agggcgagga tctccgatag cctggcggtc
    11281 agctcccgct ggtagagggg gagggccgag atcaggtttt cgaacgagaa gaagacgagt
    11341 cgaatgggga gaggatgtta ccatcctccc cccttccagg aactgtacgt gcgcctttca
    11401 gcgcatacag ctcgcgcatt tcactacccc gctaagggcg gcaacattgc gttgcctttg
    11461 caatccgggt ctgtagccta ttaacgttcg aactcacttt gtcccagtca atggagttcc
    11521 attgccgggc gagttccctg tcagtatgct tctcgccgtt cggcgtcgtt gaatgacata
    11581 ctttcacggg tatacctccc ttctttgctg tgaacctgaa tagatcaaaa atacac
//
LOCUS       DAJB01000263            4765 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_61840, whole genome shotgun sequence.
ACCESSION   DAJB01000263 DAJB01000000
VERSION     DAJB01000263.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_61840"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    544..579
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3464..3519
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          983 a         1411 c         1541 g          738 t
ORIGIN      
        1 ccttgcggag ggagccctcc cgctctgccc cttcaacccc gcagaggtag atctggactc
       61 cctctgcgtc atagatgagt tcgagcgcct ccgtcgggat agaaacagta tagcgatcgc
      121 gctcttcggc acccacatac aggagcgtgg cgttgtacgc ccgcgaaagc tcgatggtcc
      181 gcgacggctg ctcgtatatc ctccgcacgt cagcgctccg ttcgcttata cgtccctcgt
      241 ctccccgcca catatactcg tggaacggca tcccgatgac tgccgggatc ccggtgaacg
      301 acgacacccg tgagtagtag gtgtaatctc cgccctccgc ctcgacgatg atatggtcgc
      361 ccggaagatc ccgcaggaac gcgattgctg ccgcatcccc cgggtgcgag ccttcgagat
      421 aggccgcccc gtcgagggtg tggctcccgt agccgaagtc gacattgatc gcgaagggtg
      481 cagcaagaag cagcgccaca gccaggacgg gcaacagacg ccccgcccgg gcaggcatgc
      541 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgagatagac cagctcggta
      601 gcgaggatga tcaggagccc gaggatggcg agcgtatcgg tggccgcaaa gcggcggcgg
      661 gcgagaaggt agaccagcgg aacggcggcg atggccgcgg ccggatagcc ggcgagcacg
      721 aacggcaccg cggcgagcag aaggtagggg cgcttcctga tatccggagc acaaaaagcg
      781 atcagcaccg cgaggaagaa cccgtggacc agcaggaact ccaccggttc ggagggtgtc
      841 gggacgatcc cgatcccccc gatgccgggg ctgttgagct gcaggtagaa cgggagatac
      901 gttacgatcg cgagcggcgg caccgccgca agcatcatcc aggacttctc tctgtcccgg
      961 gtaaagttcc cgtaacgcca ccatatcagg aggccgaaga ccagcgtgac cggggcgtag
     1021 accagcacgt cccaggagtt gaacccgggc atggacccga gggcgagggc ggcaagcccg
     1081 cagacgagtg cccgcccccg cagggggagg gtcccccagc gcatgcaggc aaagaccagg
     1141 aggaagagca ggaacccctg gttgaagaag ctcatgacgt gtgggtgcac gtcgccccag
     1201 agcattgaaa aaatcggata ctcgttgatg gtgttcgcga tcgtccgggt gctcccccag
     1261 aggacgtcga accatgcgtc accgatcagg atgtggtaga tcgcagaggg gtttggaatg
     1321 aagagcgcga gtaccgggag ccaccggtag cgatcgagga ggagatgccc gagtgcgtag
     1381 agcgagacga cggcaagccc gagcacggtc gggaggacaa ggttgaacgt tactgtcgag
     1441 ggcacgccgg atacaagccc gagcaccccg ctcatccagt agccgatgta gtagtagaca
     1501 tccagtgtcc cccccgcata ccaggggtcg agcggcggga tggttggaag ccgcatgatc
     1561 gacgcgagga tggcgtggtc catgaacttc tcggcgtagg agatggtcgg gttgatccag
     1621 cgcacctcaa gcatgaagag gaagggtatc aggaaggcga gatcccagga gagtgcggac
     1681 tgcaggcgct ccctcgtgta gaaccgccgc gaccctgcat aaactatcgc ggcgagaaac
     1741 ggcagcaggg cgagccagac aggaaggccc gaaagaccgc agtaccagga cccgagcgtg
     1801 aagaggagga ccgatgccgg gtatgcggcg gcggccgaga ggttgccgag ggtgcggtcg
     1861 agcgccggcc aggtgccgag gtggagcagt ttgatgaccg caagccagag cagcacgggg
     1921 acgacgaact cacccgccaa atgtctcctc cttctcgatc tccctggaaa aggccttctt
     1981 taagagttcc atcgaccagt cgccgaagta gtagattgat ccgacgcccc cgatgagcgt
     2041 gaccaggttc ttgatcaggt ggtcgatgac cgcgatcagc gtcgccgtgg ctgccggcgt
     2101 tcccgcgagc ccgaaggtca gcgcgagcgc gagttcatag gtccccaccc ctcccggggt
     2161 gagcggcacc gccttgacga ggttgccgat gacgatcgca agaacgacga tgccgaacgg
     2221 gaccggctcc tggaacatca ggacgaccgc gtagcagacc agcacatcga cgagccaaat
     2281 gaggagcgat gaaacgctga gcgtaacgag ggcccggggg ttgagggagg cccgtttcac
     2341 ttcatcgagc atcctctgaa cggcaacgat gacccggttc tcggacttca tcctgcccga
     2401 ccagagaagc atggcaaaga agatgccgcc tgcggcgagg gggacgatga tgaccgtgat
     2461 gaaccagtcg gggacatcca ggacggccag aacgaacggg agcgcgacgg cgccgagcac
     2521 cgcgatcatc aggatgtcga agacacgctc gacgacgagg gacgagaacc cctgcgagta
     2581 ggtggcgtcg tcctcgtgct tgaggatgag catccgcaca aagtcgccga ggcgtgcggg
     2641 aacgatgagg ttcgccgtct gggagatgaa gatgcaggcg gtcgagaacc ggaggctctc
     2701 cttgatgcca agcccctgca ggatgaactg gtaccggtac ccccggagga tccaggcaac
     2761 aacgcagatc ccgacggcag caaagaggaa gggtatcacg gcgtgttcga gcgtcagcag
     2821 gaggtcgtcc catacgcgga agagcatgta cgcgacaatc ccgaccgcga tcagggtggg
     2881 gatgacgacc gcgctaacct ttttccacat ggagccgcca ccagagctgc acaatcgccg
     2941 atcccatctc gattacatcg cttctacgca cagtcgtccc ctctccctgc tgccatcgca
     3001 cgggaaactc tcgtatccgg tacccgttct tctgtgcccg caccagcacc tcggtgtccc
     3061 agaaccagtg gtctgcagtc accgacggga gcagggaaag aatgcggtca cgacgaaatg
     3121 ccttgaaccc gcactggtgg tcgtggagcg agctcccgag gatcgtccgg gcgagcatat
     3181 tgtagccccg gctggcgacc tcccgtccgc cgctccgggt gataatgctt tcggggagga
     3241 gacgggaacc tgttgcgata tcatagccgt cacggatggc gccgatcagt tcggtgaggt
     3301 gcggcatgtc ggtggcaagg tcgacatcgt agtagcagac gatcgatccc aatgctgcgg
     3361 caaacgcacg gttgagcgcc cgaccccgcc cgagccgctc gtcgctgtgg agaaggcgaa
     3421 cccgggagtc tttagcctcg tagtcccgga cgaactcagt gctnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng catccacaaa cctcgcgaga
     3541 accctttttg ccacttcctg cccggcgacg accgaaccgt tcatgctccg ctccgggtag
     3601 ttcggggggc tgaacatccc ggcgaggaag agcccgtgct cttcgtaggc aggcaggcgg
     3661 ttccggagtc cggtcgtgta gacggggcct gcatacgggt cgatggcgag ccggtgccag
     3721 tgcacttcgc tctcgctgac agaaaaccgc ctgcagaagt ccccaagcat cgacgcctcg
     3781 aagccctcgg ggagccgatc ggtaaaatac gacgcaaggt agacgatgtg ctcaccgtac
     3841 cacgccagcg gggcgaagtt ggtgtgcgag accaccgcac catagggggc cgcatccttc
     3901 atgttcagcc agtatatccc gtcggtcacg tcccggtcga gtgcgagggt catgcaggcg
     3961 gcgccctggt agggcaccgg ggcgatatcg agaccggcaa ggtccgcggt cacctgcggg
     4021 gggagcgtcg atacgacgca gtcataggcc tctccgttga cgagccagcc gccgttcttc
     4081 cgccggatct cctcgacggg agagcccggt acgatcgagg cgccccgtcc cgccacctct
     4141 tcctcgagcc gcgcgatgac ctgctggaat cctcccttca ggtaacccag acgctcgccc
     4201 ccggcgcccc ggtccgaccg gatagcgata cgggatatga gccaggctgc ggagacctcg
     4261 cccgcacggt ccccaaactt gctctttaag agcggttcga agaacgatgc gtagatgccg
     4321 gggccaaggt tgtcgagtat gaaatccttc gcggtgatcc cgtcgagcgc tgctgtgtcg
     4381 agccgccggg agcgcagggt cagcaggccg aggcgggcct tctcggtgag cgtgagatgg
     4441 gggtagcgca ggatctcggt tggggtggta agcgggtgga tggttccatc gacatagtag
     4501 ccggtgctgc ccttgagcca ttccagccgg tcggtcacct gcagtgtatc gaagaggtcg
     4561 agcaggctgg tatcgccggc aaagcaatgg tggtaatact cctcgatcca gtaatcgccg
     4621 atccggtacg aaccgaggca gccgcccagg atgggccttc gttcgaagag atcgacctgg
     4681 tgttttccgg cgagttcgag agccgagacc agaccggata gacctccccc cacaacgcag
     4741 atcttcataa taatcaggca ggaga
//
LOCUS       DAJB01000264           16621 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_43984, whole genome shotgun sequence.
ACCESSION   DAJB01000264 DAJB01000000
VERSION     DAJB01000264.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16621)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16621)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16621
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_43984"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
     assembly_gap    12498..12588
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3142 a         5092 c         5062 g         3234 t
ORIGIN      
        1 agaccgcttc cgcctcctcg gtcagcgcca tcaggtcgtt ccgggcgacc gtcttgaggc
       61 tgaagttccg cgacccggcc atgacctgct ggagcccggt cctgaacttc tgcgcgtagg
      121 tgtagatccc gagtgctccg agcgggatct cgccgatccg gccggggaat ttctccttga
      181 gctcctcgta gcagacgaag atctcctcga cgctgctgcc gtacttcgag acggtcttgg
      241 ggagttcgcc gtttgcgagc cacttttcga tgttcttgcc gaccatgccg gggatcatca
      301 gaccgcggcc catgcagacc gccttgacgt aggggctgcc catggcaagc gccttgaaga
      361 cgttggcctc gtctgcgaac ccgccggcga tggcgaggtc cgggacgcgg atgccccgtg
      421 ccttcagccg ctcggcgaac tggtaggcga gagactcgat gtagaacgtc gggatacccc
      481 actcgttcat catcggccag gggctcatcc ccgtgccgcc gggggcgccg tcgatggtga
      541 ggaggtcgat cttggcttcg gcgctgtagc gcattgccat tgcgagttcg acggccgagt
      601 aggcgccggt cttgagggtg acccgcttga acccgacgtc gcggagccgg tcgacttctt
      661 cgaggaaccc ttcccggctg acgaagccga ggcgtgagtg ccgctcgaac tcctttatgg
      721 cgccgtcctt aaaggctgcc tgcacttcgg aggatcccgg gtccgggagg acgatgtagc
      781 cgcgatcttt tagctggttt gcccggtcga ggctgccgac cttgatctcg ccgccgatgc
      841 actttgcacc ctgaccccac ttcagttcga tggtctccag gttgtgcttc gaggagacat
      901 actccgcggt tccaagccgc gtgtcctcca cattcaactg caccaggagt tcgccgtact
      961 tatcatggaa ctgccggtag atcccgatac ggcggtccat ctcgggcgac ttgctgacct
     1021 tgccgttgtg gtcgagttcc agtttcgggt cgaccccgca gacgttctct ccacagacga
     1081 gcgtgatacc cgctatggcg gctccgatgg cgaagtgctc ccagtttgcc cgggcgatct
     1141 cggtcgaccc gagcgctccg gtgaagatgg gaacccgcat cgggaccttg atgtcccatc
     1201 catactcggt cctggtgtct acttccgaga ataccgcggt gtcggggtta gcctcgacgc
     1261 cttcggggag tcccttcgca ccctgcgcgt agccctggat gttcaggtgc gagtagtcga
     1321 tcgggtagtt tttgtcagcg ccggccgtga tctcgccgaa cgggccggga tagagaactt
     1381 ccctgccccg gaaggaggag agccagattt cgcaggatcc cttgcaccca tcgacgcacc
     1441 ggctgcagat gccggacatg ggcacgacgt cccgggaacg gttggtcgtt cccgttgctt
     1501 cgttagcgtt aggtcttcgt agattcattg ttacacctgt gtgtggatag aaggttcctt
     1561 cctatgcata taataccttt ctgggcctga gggccgccgc atccgggttc ttcccgcaat
     1621 cgggggagat ccgccgggcc acgctgccgg ataggcggct ttgcagcaac accggttatt
     1681 tatatagttg tatcccccgg tatggctgga gcgcgacgaa agggtgaaat atcttgatgt
     1741 gcatgaatgc tttatgaact tcaggttgct atcagctctg attgtgcttc tggtcgcatt
     1801 ttcgctctgc gccgcctgca ccggctccgg cgagcccacc ggaccgtccc agacccagac
     1861 cccggcggtc acggcaacgc ctgcgcccac gacctccgtc tcgcttacgc ccggcccgac
     1921 gcagacagtg ccgccgggaa aggaggttga gttccagctt acgggcggat acccctcacg
     1981 ggttacgaac gacctgtata tcgggttccg cgggggtaag ggccaggact tcgtaacgag
     2041 catcgacgtg cgcgtgacca agtcgaccgg ggaggttatc accgataccc tgcggccggt
     2101 cgttggagac gcgggcgaac ttatcattca taatgcaaaa ggggagaacc gggttgagat
     2161 aaccgtcgct ctcgttaccg gaggctccta taaggtcaaa gacgagatcg taaaagtccc
     2221 gtaacctgca cgatctctct tttttgatga ctgattcgaa gcccggtcat ccgggaaacg
     2281 gttgaaattt gatacctgct ccccgtcgat caacggggtg gccgggcggg gtgctccccc
     2341 ctcacgcgct ctccacgctc caccgtatga gcagaccttc atcgagcctg acggcctcga
     2401 tgatgcgtaa ccgtgcaaaa tccgcttccc ggaggaaacc ggtgccgtcg gcaggcgtgg
     2461 gggcatcctt cccgccgatg acgatgttgc cgacgaacgt ctggagttcg tcgacaagcc
     2521 cctgggcaaa aagggcccag atcagggtcc cgcccccctc gaccatcagg cgccggacac
     2581 cctgccggta caactcctcg agcaggatcc cgagatcgac ggtctcgtcg ccggcgagga
     2641 cgacggtggc gtgctgccgg agggtttcca ccgttccggg gagcgcctcg cgtgagacgg
     2701 cgacgacacg cttcccgctt cccttctgga ggatatccgc atccaggggg atcctccccc
     2761 tgctgtccac cacaacgcgg atgggattct catcctctcc ggccgctctc cgcccggccc
     2821 ggagttttgg agacttgacg gtgagcgagg ggttgtcggc gagcacggtg ccgatgccga
     2881 ccatgatggc atcgctctcc gccttgatcc ggtcgacccg tgagtaatct tcgttacccg
     2941 agattctcat ctgccgccgc tctcgtgttg agatcttccc gtcagcgctc atggcgatgt
     3001 tgacgaagac aaacgggcgc atattctagg gttatatgac gatcttccat atatgctctc
     3061 ctcccgggcg gtgcgggccc cgacctggtc cccggactct cccttcaggg gccggggaaa
     3121 tgttctaccg ctaacccccg gactcaaaga attatacttc ttttttggaa aaattattta
     3181 ttctgccccg tagtgtttcg aatggcttat atatgcctct cgggatactc tctgtatccg
     3241 ctatgtccct agaccggtgc ctcatgagcc gtttccgaca agacctcttc accgggacgg
     3301 atatcgtcaa aatgattgtg gttatgtcgt tttcggcgat atcactcatg ctgacggccg
     3361 ttgcaccgga ttacaacgct taccctgtct cccccctgat atactgcatt cccattctcc
     3421 ttgccgctct ctggttcccc cggcgggggc tcctggtgac cacgctcctg gttgccgggt
     3481 ttgtacttat ccgggtatat ctgtcggtcc tcggctttgc catagacctg gtgatgacgg
     3541 ggttgcacac gatgctcttc ctctgggtct tcggggccac gatgctcttc tcacagagct
     3601 cccacctgac cctctcccgg tgcaggcaga tcgtggaaga tacccgcgac gcaaagttcc
     3661 tctgcgaccc caggaccctc cagctgctct gcgtcagcag acggtgtgcc gatatactcg
     3721 gttacacgcc ccaggaactc gtcggcatcc cggccgagac gttctgggcg gatgaggccg
     3781 acagggccag gttcatcgag gagatgaggc gggaaggcta catcggcaat atggaggtga
     3841 cgctatgggc gcacaacggc gatgcccata tggtcctcct ctcctgccgg gcgctggttc
     3901 ccgagaacct cttcgagtgc acggtcgtgg ataccggcag gctcctgggt gagaatgccg
     3961 agctcctgca gtcgaacgga cgcctgatgg aactcatcca gcagtcaaac gatatcttct
     4021 tcatgcagga tggctcgggc tgcatcctgc acttctcctg gctgcgtgcc tccgagcacg
     4081 gtctctctcc cgatgatctc atcgggcagg gtgtggatgc cctcctgccc cccgaccttg
     4141 ccgcgcagca catggagcag gttcgtaagg tgattgcgga gcagaagcat gcctgctacg
     4201 atctcgacct ggtgatagca ggcacccggc gcaccttctc cgtcaccatc gctccgtatt
     4261 ccggggcgga aggtgctctc accggggtcg tagggtcggc gcgggacacc accgagatgc
     4321 ggcggcagaa acttgcctgc aggcagatgt cctgggaggt tgaccagtgg aagggggtcg
     4381 tcaccaccat atcccacgaa ctgcgcaccc cactgcagcc gctcgtcggc tatctcagga
     4441 tgatcatcga ggatcccggg tattacggtc tcacgaagga gacggagaca ctcctcaaga
     4501 cctgccttga gtgcgccgat caggagcagg cggtggtcga gagggcggtc gagttgagtc
     4561 tgctcacgat ggactacgtc gacctgaccg ttcaggacat agcactctgc gaccttgtcg
     4621 gttccgtcat ctccggtggc gaatatgacc aggtggctca aatttgcaac gaaattccaa
     4681 aaagcgtcca tattctgggg gatcccgagc ggctttccat ggtggtggac ggtctggtat
     4741 caaatgcggt taagtataat gaaccgccag agaaaatatg gatccggtat gcgggatcaa
     4801 atgaaaacca ttatattatg gtgtgcgata atggcatcgg tctcccagag gatatcatcg
     4861 agtcgatctt tgagccgttc tacatcgggg gcgccgggag acagaacccg aaaggtgtca
     4921 gaaccgctcc ggggctctcg gttgcaaaca agtatgtcca tctgcatggg ggagaaatat
     4981 cggtgacaag tacggtggga gaggggagca ccttcaccat cagaatacca agggaggtat
     5041 gaattttggg aaacaaaatt atggtcgttg atgacgacct gccgacgctg gaagtaatgg
     5101 agctgctgct caaaaagatc aaccgtgaac cgcttctggt tcacaacggg tgggatgccc
     5161 tccgggcgat caagaaagag aagcctgcac tcatcatcct cgacgtgatg atgtccccca
     5221 tagacgggtg gcagttcctg gaggaactga agcggaacga ggagttcaag gagattcctg
     5281 tcctgctctt taccgcaaag catgtctggc ccgaggagta ttcacggtat gcggatgata
     5341 tcgtcggtgt cctggaaaag cccatctcgc ttgccgagtt gaagactgcg ctggagagag
     5401 ccctcccccg gggtgcctct tctcgcacgg acgacgatac tcgccgtatc caaaagatcc
     5461 gaagcgagta atctcttcta ggacgcaccc cgcggcatcc ttccgggtcc ccccgaccct
     5521 taccgaaagg cattttatcg accggatgtg tataccgggt agtgttcttc tcatgatcgt
     5581 cacggatctc cccggatgcc cttccctcaa ggaataccag cagatcgtgg gtagagagca
     5641 gctctcggcg ctcgaggagc ttgccggtcg cctcgcgggt gtcaggatgc aggagatcaa
     5701 ctcgacccgc tacgggggcg gcgtcgcgga gattctgatc tcgtatgtcc cgttcctcaa
     5761 ggcgctcggc ctcgaagtgg agtggagtgt catggaggcc ggaccggcgt tcttcgaggt
     5821 cacgaagtcg ctccacaact tcctccaggg ccgggacggg ttctctccgc cgatgatcga
     5881 gacctactgg gaggcacaga ggcagaatga aggacttatc gaggacaatt cggacgttgt
     5941 gacgatccac gatccccagc cgctcggtct cgtcgagttc ctgacgaacc gggaacgcaa
     6001 gcagaagagg ctgctctggc ggtgtcacgt ccagctggag acggtcccca ccgcgacggc
     6061 aggcagcatc ggcaacatct tacgaaggct ggtcgagaag tatcacgcaa gcatattctc
     6121 cagttttcaa tacctccccc tctggaacgt gccgagtttc atcatcccgc cgttcatcga
     6181 ccctttatcc gagaagaacc gcgacctccc ccgggcagag atcgacgcca ccctcacgaa
     6241 gtacggtatc gacccggaaa agccgatcgt cacccaggtc tcccgctacg acgtcttcaa
     6301 ggacccggtc ggcgtcattg aagcgttcaa gaaggtccgc cagaagaccc cctgccagct
     6361 cgtcctcgtc ggcgggcggg cgtgcgacga cccggagtgc tacctcgtcc tgcgcgaggt
     6421 ccgcgagacc gccgaggacg atcccgacat ccatatcctc gacctcccgc cggacagcca
     6481 ccgggagatc aacgcgctcc agcgggcgtc ggacgtgatc gtgcagaagt ccataaaaga
     6541 ggggtttggc ctgacggtga ccgaagggct ctggaaggga aaacccgtgg tcgccgggaa
     6601 cgtcggcggc ataccgctcc agatccgcga cggctggaac ggctacctgg tctcgacggt
     6661 ccaggagacc gccgaccgga tcctccacct cctccggcac cctgagaagg ccgcggagat
     6721 gggagcgcga gggagggagt tcgtgcggga gtactacctc cttccccggg gcgtacagga
     6781 ccacctcacg gtcatcgacc aggtgaagaa cggcaggatc actgcccggg acagcgtcat
     6841 ctgctaccac ccgcaggtgg tgacgacccg gatggccggg tgcgcggccc ggtactgatt
     6901 gatcgctccc cctctgtccg tcccgttgcc ctgatggatg agggatacac acgggcgcga
     6961 gtgttgccgg tgggagggcc gcgtcagccc ccccgtcacc cgatggggat ctctttgccc
     7021 ccgggtccgg acctggtcct catcttcttt aggcgcactt cgaggacgcc gttcttaaac
     7081 cgggttttag ccccctctcc cgttacgctc gcgggcagac gtatcagccg gctcagggtg
     7141 ccgtctccgc gttcgtggat gtagtaggct cccggcccct cctcgatcgg tccggcacgc
     7201 cttgccgtta tccgcatggt ctgcgggttg agcagggtga tattgattgc gtccccctcg
     7261 gcgccgggca gctccgcaac aacgatcacc tcatcgccgt ggtcgaggat atcgacccgg
     7321 aagtccgcgg agaggccggg gaagtcgcgg ctccccccac gggagaccgc cggcagcccg
     7381 ccgctctctc cgccccggga ctcccggttg tctgctgtct catcgatcgt tcgccgtccg
     7441 ggttcggcgg gatcgtcctc tcttcctgtc atgatccttg cctcctgcag tctggtggta
     7501 gctatcggat ggccggtcac catccgggga cctctcttga gccggcatac ttaacccttc
     7561 gtaccagggg gcaggtgcgt ccggggccgc tcatctcccg caaccacatt atctgtgatg
     7621 tatatcttgc ggtctcccgg cgataccgga acgatgccgt gccggataat cagccgggcg
     7681 ttcgtcggca ctattggcgg ggtatccggg aacggcgttc cgggtgggtc atcctgtcgg
     7741 ccgttaggtt tttgtggcgt gaccggtatg gtggagaccc ccgggggttt ccggggtgcg
     7801 ggggcgagcc gttccggtcg ccgcctgtgc cgtcgtattg ccgaaaacgc ccgtaccgac
     7861 gagttcggcg gggttatgga ggaagatgag atggctcaat tcagaccctt tgacatggag
     7921 ggcatgccga tcgaagagca gcccatgaac tggaaggata tggtcccggc cccgtacgac
     7981 aagaaagcgg tcgacgccta cacccggacg cggatcatcc tcatgaacgg tatcgagaac
     8041 aacgccgtcc tgatgtcgca cgcgatcgag cggatgcacc cggaccccga ggtgaagaag
     8101 gcgatggccc tgatgcggcg gatcgactcc cagcagcagg agacggtcaa ctggctcaac
     8161 ccggcggacc agtcgatcat cgagaccacc ctcggctacg agcaggtggc ggtcgacctc
     8221 accgcgaacc tggcgaagaa cgagccggac ccctacgtga agcagacgct ggactttgcg
     8281 ctgcttgagg atttcgacca cctctaccgc tacagctgcc tctacgacta catcgagggc
     8341 ggcgaccccg aggcgatcgt ccagggcaag accgaggtca aacccggccg gccgacgagc
     8401 atcgagcacc gccaccccga cgactcgatg cgtaagcact acgacaagga tacgaccgac
     8461 atcaagacaa agatgaacta catcacgatt gtctcggggg agcagcagac gatgctctac
     8521 taccggtcac acgggttcat gtatcccgac gagatcgcgc ggcagctcta cgcagagatc
     8581 gcggagatcg aggagcagca cgtgacccag tacgggctgc tcggggatcc gaacgagacg
     8641 atgcttgaga agatggcgat gatggaactc aacgaggcct acctctacca ctcctgcgca
     8701 cagaccgaga gcgacccgag gatcaagggg atctgggaga acttcacgaa gatggaggtc
     8761 acgcactttt ccgagtgtgc gaggctcatc aagaagatcg agggtcggga tatccaggac
     8821 ctcgtgaagg ccgatgtcat cgagcccctg atagtctttg agcagaacaa ggattacgtc
     8881 aacagcgtca tcgatgctca actcgacctt gcgccgaaca acatggagtt cgtgcggatg
     8941 cgcgacctcc cggacgactg ggcgagtttc ggctaccagc gcgtcatgaa cgcaaagggc
     9001 gttccgagcg aggagattgt cggcaaagcg ggccgggagc tcgcggaacg cgaccaggcg
     9061 gagaatatcc ggagggtcaa gcaggagatg gcccgccggg tcgagatggg tatggcagcg
     9121 gtgcctgccc ggtaaatact ctttttttac ctgcccgtct cgcgacctcg cgattatggg
     9181 ctctgctctg ccgatctgct ggattcgcct cccctggcct ccctgagcgt gaacaaaaac
     9241 gctgcccctt ctccgtgatg gcccgatacc cggtcttcga cccggatccg gccgccgtag
     9301 cgggccacga gcatccggca gatcgagagc ccgaggccct ggccgctccc ccgggccttt
     9361 ccctgctcga accgggaaaa gatcgcctcc cctgcatcct cgatggtgag ttctatcccg
     9421 acatcaggcc aggtgcgatc gataccttta tgatatttcg cgcatatgag taatatatca
     9481 caacagaggt gaacaaaaat gccaggattt gatggtacgg ggccccaggg tatggggccg
     9541 atgaccggtc ggcgcatggg caggtgcgtc ctgccgccgc agccgccggt ggggggcagc
     9601 cctgctggag agacgaacga gaccacccag ggggtaccgg ctcagcagcc gctggtttac
     9661 ggccttggcc ggggcgggat cccttacggg tgtggccgtg gccgcggctt cggtggcgga
     9721 cgcgggcgcc ggtggtgaac gcttgacgca agttggtgcg ggccggtctg ctatccggcc
     9781 gcccctcgcg gagccagccg gggtgtagtg agcatgacgg cagtggttga tccggaactc
     9841 tgcaccgggt gcgaaacctg tgtggatgcc tgcccggctg cagcaatccg aatggaagac
     9901 gggaaagcaa aggtcgacgc cgggctctgc atcgactgcg gaacctgcgt cgatgagtgc
     9961 ccgtcaggag ctatacgtct tgagtaatgc cggccgggca ggatccagcc ggtgaaaaaa
    10021 ctctttttat gtgatttaac gcaaatttct acgcctgcag gatccgttgc cgtcatatcc
    10081 ggatacggat cgtccatacc tgaatttaga gcgtgagtgg aagagtaggg gaagaagggg
    10141 tttggtgccg gtcccacagg gaggaacacg gtgatgcgtg cagacaagga gaaccctgtc
    10201 agcctgtggc taccagcacc gccatccgcg gccccgtccc ccaccgaagc cgcggccacg
    10261 gccgccacct cgtggcaacc ccccgcggcc gagaccgtag accggtcccg gatagggtga
    10321 gtacggaacg cgataacccc caatgggccc ccacacgggc ggccactgcc tccgtgcggt
    10381 ggggttgcaa tagccgaacc cgccgccggt catcgggccc agccctcctg gtcctgtacc
    10441 atcaaatcct ggcataattc atctcctttg cgggcacact cccgcccgct attattactc
    10501 atatgttcta aagtatataa atatttgcca aatttacata gcaggcgaaa ctgatggacc
    10561 tccccggaac ggggcctgca aaaggcggag tgcccccact tgcctgaatg gcaacaaaaa
    10621 taggctggtt cgttccggat acgggttgtc tctcgcacga gaggatcgaa gccgtcccac
    10681 cggttcaggc acttcctgcc ctcgatgctg gctgttgaga gaggatctat ccttcgctcc
    10741 cggagttcgg caaagcgttt cttgcttcgc gggcaaaagc aagcgtcctt tccgggttgc
    10801 ggacaccgtt tttcttgacg ccggtatgca cgtcgacccc gtacgggcgg acctttcgta
    10861 tcgcctcgct cacgttctcc ggcgtgagtc ctcctgcaag tatgacggga attgtcgagt
    10921 ttgcgacgat tgtcgcgctg atattccagt cgtgggggat tcccgttccc ccgattttct
    10981 ctctggtcct ggtgtcgagg atgagcgcat cggcataggg ttcgtagagt tttcccgttt
    11041 tgatggccga ttcgtccgtc acgtgaacgg ctttcacgat cttgaggtac gggagagaat
    11101 cgcggatggc atccagttcg gacggttcga cggtgttctg gacctgtaac gccgtgcagc
    11161 gcgagtctct caccagctcg atcaggtctt ccgtctcctg cagatgggtg actgcgaccg
    11221 gttcgatgaa cggcgggagt tgttgtatca tcttcgcagc ttctgctgga gttacgaaat
    11281 cactgctctg gtgggtcacg cccataatga aacccatagc atcgcagcct gcatcgattg
    11341 cacactgcat atccgcaaga ctggctgttc cgcagaattt gacacgcatc ggaaactctc
    11401 tgtcgttatg gcagataggg tatttgcaga tagcgaaggc cgcgacgaca gatccatggc
    11461 ggcgatcagc gctatgcgcc ggtgctgcga ttgtagttat gtaaacgaag ccatcaggaa
    11521 agttgcttgg agtgcccccg cccggatttg aaccggggac aaccagatct tcagtctggc
    11581 gctctcccag tctgagctac aggggcttct gccttacaat gttgtcggtc gttcctaata
    11641 aatactgtgc ttcgcggacg gcagtctccc gggccggatg gcaggagaca tcgggcttaa
    11701 gaccgcctgt ttatcacatc gcacaccgat gtataaccaa tgggccggag agatcacaag
    11761 ccggattccc ggggcacgaa cctgatgcag gcgctctccg gaaggacgga gaccgtcgtg
    11821 cacctcacgc ataacgacct cgacgccgtc ggcggcgatg ccatacaccg caggaagtac
    11881 ggggaggtct tcaccatctg gtcttcggtc ggcaagtttc tctccctctt cgatgccgtg
    11941 gccggctctc cgggacgggg cgacctcctc agcatatcgg atatcggcta ccagcagggc
    12001 gtcgagcaga ggctcgccaa ggcccggtcg aacgggtggc ggatcgagtg gcgcgaccac
    12061 caccgctgga aggacgagga gatccgggcc gtcgagaaga agacgagcct cctccacatc
    12121 gacgtcgccg tctgcgccac cgggatcgtc gcacgcgacc ttgcccccgg cgacgccgtg
    12181 gcggaggaga ttgcgcgggt cgtctgcgac tacgacctct ggaagcacca ggacccccgg
    12241 tcgaagatgc tcggccaggt ggtgatgcgg aagggctgcc gggagtacgt ccgcgacaac
    12301 cttgtccgcg gcaccatcat cgatgcgagg gtggagagcg agtacgcggg cattgtccgg
    12361 gagatggagc gggatatcaa aaagagcctc cggcacacca ccgtcatcgc ggacggtcgc
    12421 taccggatcg cgtttgctcc cctctacggc tacccgagcg agaccgccca taccatccgc
    12481 gacgaactcg gcaccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccttgcccgg
    12601 gtggcggaat ctatcgagga atgaacgccc ggtcgaccag attactccag tagtccgggc
    12661 agccgtcctc gcagtagccc ccggccacaa gcatatccgg gtcggtgaat gcggcggcgg
    12721 ccgcgaggtc tttcacctcc gcatcgagca ggatcaactt ccggacacgg aaccgctcca
    12781 tcaggtccgc tgcccggccg aggtcttttg ccgggaggac aaggtcgccc tggtactcga
    12841 gcagcagttc caggtcgaag gtccccgggt ttgccgggaa gaagaggatc tttttgccgg
    12901 cgagcacctc gagctcgatc gcgtccgccc tgtccgcaac caggatgtgc cccggcaccc
    12961 cgatatcgcg catctcccgc gcaagccggg catacccgtt tgcgatggtc tcggagaact
    13021 cttcttcatc gccgatgtag gcgtcgcgga acccgagcat atggggtgcc ggaagagaga
    13081 accaggcatc cttccgcctc tcccggacga actgcacatc cgcggccaga gcggccggct
    13141 cgatacccgg ctcgtcgacg agcaccccgt cggtcatgcc ggagaacgct tcccgccagc
    13201 gctcgccgta gaagacgccg ccggcggcgg gcagccccac acccttctgg gcgtcgagcg
    13261 agcgatcgag ccgataggtg gtgaggtcca cctccttccc cctcttctcc ttcagccact
    13321 ccgcgagctc agcgaccgtg ggcatcccta cctcggagcc gaaggacctg accggtattg
    13381 taatacgtcg tgccatgcgc tggtgtcttt attagcatcg caaccagagt agataaggat
    13441 gagtggtaag ccgttgctgg taacgtgtgg gctcccgtac acgaacggtc cctgccatat
    13501 cgggcacctg cggacctatg tgccggcgga tttttacgtg cggtatatgc gccggtccgg
    13561 ggaggaagtc gtctttgtat gtggctccga caaccacgga acaccgatcg tgatcagcgc
    13621 cgagcaggag ggctcgacac cccgcattct ctcggagcgc tatcatcagc acttcttcga
    13681 gacgttccgg cggatggagg tcgtcttcga ccggttcggc atgaccgacg atgccatgaa
    13741 ccacgagacc acccaatcga tggtgcagcg gctgatcgag aacggctatg tctacgccaa
    13801 gaccatcagc cagagttact gcccccactg cgaacggttc ctcccggacc gctacgtaga
    13861 ggggatctgc ccccactgtg gggccgtcgc ccggggcgac gagtgcgacc agggctgcgg
    13921 gcagcacctc gagcccggcg agatcaagga tgcggtctgc aaggtctgcg gcgggaaggc
    13981 ggagtaccgg gagcaggagc actacttctt caggctctcg gcattccgcg agttcctcct
    14041 cgactacctc cccaacctcc ggggcacgtc caccgcccgg aactacgctc tcgggtgggt
    14101 gaaggagctc ctgcacgact ggtgcatcac ccggacgctg gactggggcg tcaaatttcc
    14161 ggggagcgac gacctcgtcg tctacgtctg ggtcgacgcg ccgatcggct acatatcgtt
    14221 cacgaaggag tgggcacaga gtgcaggggc cgactggaag gactactggt gcggcgacga
    14281 gaccggggtc acgcacttca tcggcgggga catcatctac caccactgca tcttctggcc
    14341 tgccctcctc aaggcggcgg gctacggcct cccgaatgcc gtggtcgcga gcggtatggt
    14401 cacgatcgag ggcaagaagt tctcgaagtc ccggggctac gtcgtctgga caaacgacga
    14461 ctacctggac caggggcttc cggcggacta cctccgctac tacctcctct cctacacgaa
    14521 ccacaccaag gaactcgact tctcctggaa ggtctacgcc gagcgggtga acaacgagat
    14581 cgtcgggacg ctcgggaact tcatctaccg gacgatgtac ttcgccgaga aggagttctt
    14641 gggcgtgccc aacctccctc cccggccgga gatcgtcgag gagatcgagc ggtcgttatc
    14701 cgtggtcgat gccctgatgc gggactacga cttcaagaac gccgtcgact cgatgatggc
    14761 tttggcgtcg tttggaaaca cctacatcca gaacaacgcc ccctggaaac tgatcaagga
    14821 ggaccggaca gcggcagagc aggtgatcgc cgactgcctc cagatcgtga aagcgctcgc
    14881 cctcgtcttc gaaccgctga tgcctgacgc gatgcagcgg gcctggacga tgctcggctg
    14941 cgacgacgat atcgcggacc atcccatagc cgaggccacg gtgccgatag gagcacgcat
    15001 gcttgcaaag ccttctcccc tcttcgtgaa gatcgaggag gcccaggtca aagacctcga
    15061 gaaggtcctc aatatgcggg tcgatgaggc ggatgcggcc gccaggccga agaagcgcac
    15121 cgtctccatc gaggagttcg gcagcctcga gatccggatc gcaaaggtcg tctcggccga
    15181 gccgatcaag ggctcgaaga aactctacaa actcatcgtc gacttaggag acgagaaacg
    15241 ccaggtggtg agcggcatcg cgcagttcta caccccagac gaactggtgg gcaaagacgt
    15301 ggcgatgata gcaaacctcg ccccggcaaa gatcttcggt gtcgagagca ggggcatgat
    15361 cctcgccgcc ggtgaggagg cctccctcct cgtccccctc cgcccggtca gcccggggac
    15421 gccggtccgt tgatcaggac tacctgatcg atcctgtttt tcatcgagtt cgtatcgtag
    15481 acgacgatgc gccactgccc gccgggcgag aacggacgga tgccggggtg gtaggcggct
    15541 gtaaagatca tatccggctc gtcggtgatc cagtagccgg acgcggccgg ccggtcgtct
    15601 ctgggaaaat tgaagatgta cactacctgc ccggcaccga gcgtcgtgtt ctccaggatc
    15661 gcggtctcca cgccccggag ggtcccttcg gggtcgatga ggtagatccg gcacctggtg
    15721 aggttgatcg cgctctctgt gccggatatg cgtattgccg gggcatcggc atacccggtg
    15781 acggcctcca ccgggagggt cagggtccgg cctgcgactc cggtctcctc tcccggtatc
    15841 aggagacctg cggctacgac agtcatcagc accgtggcta tgatgatggc acagaccgtc
    15901 gccgtcccgg tggagatagg cccccgtctc acgagcagcc tttgggcatc agcctgtatt
    15961 tgaaactatg ggtggagcgg cgcgaggagg ggacgagcag aaagcagagg catgttgtga
    16021 aaaaaagggc tctgttgaac ccctattctg gtcttcctcc ctatcctgat gaggctggtg
    16081 ccggatccgg cctgtccccg ggacgacctt tttttctcgg gcatctcctc atgctgtgga
    16141 ccgtggtggc tatcgccatg ggggtggggc tgactgggag gggtctcccc ctcccctgtt
    16201 tcgcgcgaag atccgcataa ctcagacccc accccgcccg cgcttcgcgc tcctcccccg
    16261 ccccaggggc gggggcagtc atggcgatac ccggtggaaa gccgtgcccc ggagtgcggc
    16321 catcgggaac aacatggggg tgttcaacaa agccgaaaaa agggaatcgg gcagaacgtt
    16381 ctagagacgg tcgagtgctt ctctccctgt catgccgacg gcaacgccgc gctcgcatgc
    16441 agacctgttc gcccccttga tctcgccgcg gaggagatcc tcgacggtct cgatcgagga
    16501 tgcgcttcct gcaggtttca cacgcgctgc ggggtatccg aacttttcaa gcgcgtcgac
    16561 gtcaaacgcg ccgcaaccga ccatgcctgc atcggtcacc actgccacaa ggtttaccgg
    16621 g
//
LOCUS       DAJB01000265            9352 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_14124, whole genome shotgun sequence.
ACCESSION   DAJB01000265 DAJB01000000
VERSION     DAJB01000265.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_14124"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1695 a         3135 c         2903 g         1619 t
ORIGIN      
        1 tttatacctc atgttgggtg aattataata gaatacccaa gaggatatac ctatcgcagg
       61 aaggaagggc ggctccgctt tcatccccgg cctgaagacc ggggacttcc cgctccgccc
      121 ccttcacccc cgcaagttaa aagatctcgc ggggttctcc cggataatcc tcctctatca
      181 cttccaccgc tcggagggct acgcgctcga ggtggtcccg ttcctcgaca aaacgcccca
      241 cggcgtcttt gcgacccggg ctccccgccg ccccaacgcg ctcgggctct ctatcctcaa
      301 actggtcgcc gtcgacggcg cgacgctcac catcgaggat gtggacatcc tggacggcac
      361 cccggtcgtc gacatcaaac cctacgtccc ggcgttcgac gcctaccccg atgaacggtc
      421 cgggtggctg gccgcagccg gggaggaccc ccgcaccgtg cggtcggacg gtcggttctg
      481 ctgaccgggg agaaccgtaa catttaacac gacgtaggtg aagtataatt atttaacata
      541 acttaaaagt aaaagatagt actttaatat ggtaagggtg ggtactgaat ggtatccatg
      601 acaaagaacc tcgttgcact gattacgctc gttatccttg ccgccctcct ctgcacggca
      661 ggatgcacct cacagccgac gatcaccgcc accggcgaca acaccacccc cgccgggacg
      721 gatacctcac tcttcgtcta ctccggcgca ggcctcaaga agccgatgac cgcgatcgga
      781 gaggcattca ccgggaagta cggcatcggc gtcgattaca ccttcgccgg ttcgggcacg
      841 ctcatcgccc agatggacct ctcccggaag ggcgacgcct tcatccccgg cggaacgccg
      901 gattaccgga cagcgcagga gaaggggctg gtcggagaac ccggatacgt cgcctaccac
      961 gtcccggtga tcgtcgtcca gaaggggaac ccgcagaaca tcacctcggt ggacgatttt
     1021 acccggtccg ggctgaagat cgccctcggc gccgcgaaca ccaccgcaat cgggaaagcc
     1081 ggcgacaaac tctttgagaa gcacggtatc ctcgacgccg tcgagaagaa tgtcgtcctc
     1141 cgggccccga cgatcaacga ggtggtggtg gcgatgaaca tgggcaccgc cgatgccgcg
     1201 ctcatcacgc tcgaccaggt gaactccgcg acgatggata cgatcgacct cccgcaggaa
     1261 gacggcctcg tcctcatcgt gccggtaggg gcgaccacct tcacggagca gccggacgcc
     1321 gcccggcagt tcatcgagtt cgtcacctcc gacgagggga aggcaatctt tgcaaagcac
     1381 gggttcccgg cctaccccga cccggcgtac gcggggatcg aaccatgaac agcagagagt
     1441 actcctgcag gaccatcggg attgcccgga cggcgttctc cgacccggcc gccaccccga
     1501 tccaggcggc tttctccacc gcccgcggga cggtggaggt cttccccgag ttccgggacg
     1561 gcctttcggc gctcgcgggg ttctcccacc tcatcctcat ctaccggttc caccgggccg
     1621 ccggtgaaga aatcgccgaa cggccgctca tcgacggcgc ggaaccgcac ggcatcttcg
     1681 ccacccggca cttcaaccgc ccgaaccggc tcgggatatc ggtcgtcagg cttgccggaa
     1741 ttgacggcgc cacgcttgcc gtcgagggcg tcgacctcct ggacgggaca ccggtcctcg
     1801 acatcaagcc ctacatcccg gcgttcgata gcatcggagg tgcgacttca gggtgggtga
     1861 cgccgcaaca cgtcgggcag atcaaaagac agagcgcgtc cttccaggtt gattagccgt
     1921 gttcccgaac agggatgccc tctcccgtcc taccccattt aaaatcctct ttctcgctgt
     1981 cctgttctgc ctgatcggtt acatggtcat cgtcctcgtc ggcctcgcgg cctacccacc
     2041 actcccggcc ctcatcgaga gccttgcgtc cccggagatc attgccgcgg tcgaactctc
     2101 cctcgtcacc tcggcggtct ccaccgccct ctgcatcctc gtggccgtcc cggtcgccta
     2161 ctccctcgcc cggttctcgt ttcccgggaa aagcgtcgca aacaccctga tgaacctccc
     2221 gctcgccctc ccgcccctcg tcgcgggcgt cgcgctgctc atcttctacg gcccgtcgac
     2281 attcgggaag atgctctcat ccctgggact cgacgtcgtc tacaccccgc tcgggatcat
     2341 cgtcgcccag ttcttcgtca acgtgcccta tatgatccgg gtcacccggt cggccttcga
     2401 gacgatcaat ccccgctacg agcacgtcgc ccggacactc gggtgcaccg aatggggtgc
     2461 gttccgccag gtcaccctcc ccctcgcccg taacggcctc gtcgcgggcc tggtcatcac
     2521 ctggtcgaag tccatcggcg agttcggtgc ggtcctcatg ctcgccgggg ccactcagat
     2581 gaagaccgag accctgccca tcgcgctcta cctgaacatg tcgacaggag acctcgacct
     2641 tgccatcgcc gcatcggtca tcctgatcgt gatcgccatg gcgtcgctcc tgatcttcga
     2701 gcgctgtgcc gacacccggg ggatcttctg atgctcggga tcaggggtct ctccctctcc
     2761 ctcggggagt tttccctcac cgatgtggac ctgaccgtcg ccgacgccga gtacttcatc
     2821 atcctcggac cgaccggcgc cgggaagacg atactccttg agacgatcgc ggggatctac
     2881 gctcccagag cggggacaat caccctcgac ggccacgata tcacccgcac cgacccaaaa
     2941 gaccggggga tcggtatggt ctaccaggac tacatgctct ttccccacct caccgtcggg
     3001 gagaacatcg ggttcggcct tctgcagcgg ggggatgacc ctgcccggat acgggaggca
     3061 gtgcgggagg ccgcagacct gctcgggatc ggccatctcc tcgagcgcac gccggggacg
     3121 ctctccggcg gagagcagca gcgagcggcg atcgcccggg cgctcgtcct ccggccccgt
     3181 gttctgctcc tggacgaacc cctctcggca ctcgacgccg tcacccggga tcggctccgg
     3241 aaagaactga gaacaatcca ccgggcgacc gggacgacgg tcgtccacat cacccaccac
     3301 ttcgaggata tattcgccct cgccgaccgg gtcgcggtga tgcaggacgg gagagttgtc
     3361 cagacgggca cgcccgacga ggtcttccgg aagccggcca ccgagttcgt tgccgccttc
     3421 accggcatgg agaatgtcta ctgcggggtc tcgatggtcc gggacgggga ggcgacgatc
     3481 gacctcgggg cgatcaccat ccggaccgtc accgagatcg agggcgatgt ctgtgtcggc
     3541 atcaggcccg aggaagtgat cgtatcccgc gagccgttcg agtcgagcgc cacaaatgtc
     3601 ttctttggaa cggtgacaga ggtccggcag aacggtatgt tttcgcgggt cgtcgtggat
     3661 gcgggcctgc ccttcgtcgc cgtcctgacc cggcagagca tcgcccgcct gggcctcgcc
     3721 gagggagaac cggcctctgt caccttcaag gcctcggcag tccacgtctt cacccggtag
     3781 ccgccggagg atttatccgg gctccacgcg cagggacgac cgatggaaac cgacgagatc
     3841 cgggaagaac ttgcctttgc cgccgcggag gccgccctcg ccgcccggtt cggcataccc
     3901 gccgatgcgc tcatccctct cctcttctcg ctccagtacg ggggcgactg gagctatgcc
     3961 tcgggggacg tcagtgccat atcggccgtg acaaagacca cggtctacga cgaagcgagc
     4021 cagaccggct actccctcga ggagatctac ctcttcatcg acccggcgct cctggaacgg
     4081 gaggggacgg tccaccggct cgagaagtgc ggcagcgctc cggcccggct cctcgtcaag
     4141 cggccctacc gggtgcggct cggcgccgaa cggaccatca aaatgactgt tcatccgctg
     4201 gagcgcgaga tccggaccga agacctcggc accgtggaga tagccttctc gggctccacc
     4261 gcctacggta tcgaccacga gctcgagcac ctcgccgggc gggagatccg cggggagaaa
     4321 ctccgggtct tccggttcgg gtgaggaggg gatcccccgg cgcgtcccct ggttcccggg
     4381 cgcggggtcc cgcgcccggg tcctgaaaat agtagggaca tgtcctcttc cgtgagatcc
     4441 ggctctatca gccggttctc agactttcac gaaccggctc ttcggtgcgc cgcaccgggg
     4501 gcatttccag ctggcaggaa ggttttcaaa ggctgtttcc ggctcaacgc cgtgggcagg
     4561 atccccgatc gcggggttat agatatagcc gcagaggctg catcggtacg aatccataac
     4621 tggttctcct tctgccccga ccggccgcga ggtcggcaga gcacaagatc tcatttactc
     4681 ccggacggag gataagtgct ccggtcagga gaccgggagg gggagttccc tgccggtgca
     4741 gccgtcgagg atgatcgagc gttccccgtg gtccgatgca aaccggatca ggtagaccgg
     4801 gtagtagatc gtgtcgaacc cgacgatctc cgccgtgggc tcaagccctt tgaggataat
     4861 ccggagagag ggttccgtga ccttcgcctc gaggggctcc ccccggagtg gctccatggg
     4921 gagatccgct ccccgccgga gagaggagag cgccgggacc tcctcttcaa gcagcggcac
     4981 gtagaccacc gtatctccta tcgtcttcat ctccgtgaca agtttcgcct ttgcaaggct
     5041 ttttatcgcc ttcttcacca tgccgggcgg gagatgggta tcggcctcga tctcggccat
     5101 tgtcgagccc ccgttcgcga gcccggcaac gatcttcacc gcagcctcat cgaggtccag
     5161 aagttccgaa aaccccggca tgaccttcag cccccggtcc acctcaacga tgcagccggt
     5221 aagcgcgtca atgacgaacg acactgtctt cgtcgtcttc cagatgagcc cgccgatgta
     5281 gcggacctcc acccggtgaa gcggccggta gacccgttct ccggagacaa tccgctcctc
     5341 gggctcgagg aagaggaacc ggcgtttccg ttttgccatc gcgatatcaa gcgcctcctc
     5401 gcggagcagt accggaacca ccgcgttcgt agcggcgggc ggttccaccg gaccttcctg
     5461 gtagccgccg ggacacggtt cagccggggc cgcaccggcc ggcggggcgg gcacaagacg
     5521 gggtgtcagc ccccggtgct ccgtcaagcg gtcgccaaag cgcatcctca ccttctcccg
     5581 ggagagcccg cccatcacga agaactcccc cggttcgagc tccgcgagtt ccccgacctc
     5641 ttttttggac gaaaagaaga ggctgaccgc tttgaggtcg ttttctatcg agagcttccc
     5701 gatgatctgg ttgttgcact gcgagaggac gttctttgcc accagcgatg gccgctgggt
     5761 cgcgaccagc agcccgagcc cgcgcttgcg cccccggcgg gatatctcct cgatcttctt
     5821 gatggagtcg cctgactgcg ggatgaactt gtctgcctcc tccacgatca gcaggaaggg
     5881 cttctttaac ccgctctcga gatcgtagag tacgtcggcg aggtgagtca cctcctcgcg
     5941 catatcggcc tccgagacgt cgaagatcac cgccgtccgg gagcagatag cattccgcat
     6001 cagttcctgg aggtttgcct gcccgatatc cacgtcgcag ccgtcggcgg agccaaccca
     6061 gaggacgggg aaccggtccc tgagcgagga atactctccc tccgtgtcga tgagacagaa
     6121 cccgacccgc gccttcagga gctgctcgca gagaactgcg atgctccagc tcttcccggc
     6181 acccgactgc gcgatgatac aggtccttcc cgtgaccagt tcctgggcat cgacggcgat
     6241 ctcgcggtcg ccgtcttttc caatcaccag ctccatccgt taaaaatatc acaggatgcc
     6301 cggagtttta acgcttcctc ttcgttccat aggccccttt ttttaaaatg gaggcgaatt
     6361 tatcaacatt tattcctacg gagcgagaga acgaccccaa aggccgagag gactgcgaac
     6421 gcaaggaaga tcaactgcac gctcaggagg aactccggga atatcgccgg agtcacggtc
     6481 gtcgacccca tgatgaccgc aaagaccacg aggaccgccc ccatgctcag catcataccg
     6541 agcgaccgca tcatcgccac catcgccgat gcgctcccga agaactgctt ctccacgcag
     6601 cccatgatgg cggtggtgtt cggggacgag aagagcccga ggcccagacc cagcaggatc
     6661 aggagggcga ggatcacgac gataggtgtc gtctcgccga gcatagagaa gccgaagagg
     6721 cccacggccg agagcgccat ccccaccgag gcgaccaccc cggcctgctt ccggtcggcg
     6781 aggcggcccg ccaccggggc gacgaagacc tggatgatcg gctggaccag gagcagggtg
     6841 cctgcggcgg cgggttcgta gccccgaatg tactggaggt agagggagag gaggaacgcg
     6901 accgcaaacg tggcgctgta gttgatcagg gcggcgaggt tcgaggcggc aaagacacgg
     6961 ttcttctcga ggagcgagac cgggaggagg gggcagggct gccgctgttc cacccggaag
     7021 aagaccgccc cgagcacgag ggccaccaga aggaggacca cgccggccgg ggtggtcgcc
     7081 gaagccaggc ccaggaagag gcagaggatc agggccgacg agagggccag ccccgggacg
     7141 tcgaagtgct cgggctgccg ttcgtttaag aacgccggga actttttggc gtagaagaga
     7201 accggaacgg cgaggagcgc ggtcacgatg aagatgctgc gccagccgag gaactgggtc
     7261 aggatacccc cgaggaacgg cccgaacgag agcccggcgt agacggcggt ggtattgagc
     7321 ccgatcgcgt agccccgctc gccgggcggg tagaggtgcg tgatcagggc cacgctgttc
     7381 gcgaagatca tggcgccgcc ggcaccctgc aggaagcgga agacgaggag caggccgagc
     7441 gtgggcgtga agatggtgag gatggagccg gcggtataga ccacgatccc gatcaggaag
     7501 acggtgacct tcccccggga gtcaccgagc cttccggccg ggaggaggaa gatgaccgac
     7561 gagaggagat aggcgctgga gacccaggcg agcgtggcgg cgtccgcgga gaactcgctc
     7621 ccgatggccg gcagggcgag gttgaccgcg gagcccgtga acgggttgag gaacgtgacg
     7681 acggtgacca ggatcaggat gaatcgccgt gaatatgatt gagcgtcttc ctgctgcact
     7741 gctgtcactc atacctcccg aagagtccga tccggtcccg gggtctgcgc tataagagta
     7801 ttcacagcaa gcgcaaataa gggcactgtc cctgccgggt ccgtgcagta ccttatgaga
     7861 acggcccccc aagacccccg ggaacatgcc cgaaccttcc cccgatccct ggcagaaggt
     7921 ccgcgacctc tcgatccggc tcatcctgct cctcggcgtg gcaagcctcc tggggagcct
     7981 ggtctcgaac ggtgcccgga gtgtcaccgg tccctacatc ctcctcctcg gggggagtgc
     8041 ggcgatcgtc gggctggtcg ccgggtccgg cgagttcata gggtacgcgc tccggtctgc
     8101 caccggtgcc tacgtcgacc agagtcaccg ctactggagg gtggcaatgg ccggctacgg
     8161 cctgctcgcc gccatcccgc tccttgccgt cgtcggccgc tgggagactg cggccgtcct
     8221 catcatcgcc gagcgggtag ggaaagccgt ccggacaccg gcgcgggaca cgatcctctc
     8281 ccacgcgacg acgaccgtcg ggcgggggtg ggggttcggg gtccataagg cgctcgacca
     8341 ggtcggtgcg gtcatcggcc cgctcgttat ggtcgccgcc ctggccttga ccggcggata
     8401 tgccccgggc tttctcctgc tcggcatccc tctcgccggt gttgcaatag tcctcctctt
     8461 cgcccggtcg gcggtgccga agccagggcg cctggaggtg aacaggggaa ccagagagaa
     8521 cggggatgaa ccgcccggga tcatgccgta cgccacgttc atcttcctcg gcatggccgg
     8581 gttcgccagc ttcccgctca tctccttcca cttgaaagcc cagtcgatca tccccgacgc
     8641 cgccatcccc ctcttctacg catccgcgat ggtggtctcg gtcatcgtgg cgctgctcgt
     8701 cgggcgggtc ttcgaccgca tcggcaccca tgccctcctc gccatcccgg tcctcagcac
     8761 cgcgacggtc ctgcttgcct tctcgttcaa ccccggcgtg gcgatagtgg ggtcgctcgt
     8821 ctggggggcc gggatcggga tcttcgagac cgttctccgg gcatcgatcg cggagtctac
     8881 gacggcggac cggaggggac aggtttacgg cgccgtgagc gcggtcttcg ggacggcgtg
     8941 gttcgtgggg agcgcggtga tgggggtcct ctacgacgta tccctcgggc atatcatcgc
     9001 atacgttctc atcgtcgagg ccgcggcggt ggccgcctac ctctggatgt gccgctcccg
     9061 catcgtggtg aggttccagg aggggatcgg ggacctcatc ccgtaacccg ccggcgcgac
     9121 accatccatg ccttcagtgc cccaccgatg gcgccgccga cggcgccgag gagcccgaag
     9181 tagagagcca gcacgaagag gaggacggcg atcccgaggc cgatgagggg gccggcagga
     9241 ccgaggaacg ctgtcccgcc gacgacggcg acgaccgaga atatcgcggc gacgatgagt
     9301 ccacccatga gcccgccgag agcgcccctg accgctcggc agtgctccag ca
//
LOCUS       DAJB01000266           19536 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_49545, whole genome shotgun sequence.
ACCESSION   DAJB01000266 DAJB01000000
VERSION     DAJB01000266.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19536)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19536)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19536
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_49545"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         3810 a         6082 c         5961 g         3681 t
ORIGIN      
        1 actccttgct acatctcttt gaacgcccgt cacacctgct tgacgggatg ggatcagggg
       61 cgtgcagcag ggggcgcacg acgcatccga tcatcgagac cgggaatgtt cagggttcaa
      121 gcggagagcg ccacgaagta tatactcttc cggagggaga ggtggttatg caccggatca
      181 tcgactggaa cgagctctgg aaggcaatcc acgtgagttc gcccgaacgt gtcgaaaaag
      241 accgcgaccc cgctgccgtc tgggataagc gtgctgccgc ctaccagcag gccacccgcg
      301 gcgagaagcg agcgacggag caggaactcg ggaccctgga tctgcgaccg ggcgacaccg
      361 tgctggacat gggtgcgggg acaggacggc ttgccgtgcc gatagcacgt accgccgccc
      421 acgtgacggc cctcgacccg tcgggcggca tgctctccat ccttcgggag cggatggcgg
      481 cggagggacg gacgaactac tcctgcgtta agatgcgctg ggaggatacc gtgatcggca
      541 gggatatcga gccgcacgac gtggccatcg cggcattctc gctcggcttc tacgacctcg
      601 ccgccgccct gcagaaactc gacgccgcgg ccctccggac ggtctatctc ttctggcacg
      661 cgggcgagtg gcggagcccc gatgagatgg cgctgtatag gacggtcttt ggggaagaag
      721 cggccatgca gaaggggtat ccggactata gctatcccgt caacatcctc cacgatgccg
      781 ggatctaccc gaacgtcagg atctatcatg ccctctggga cgcggtctac gactccgtcg
      841 aggatgccgt ccagacctgg gcggcgatgc acaaccccga tctcgcggat ctctcccccg
      901 tcagggagta cttctcacgg acactccgcc gggacgaatc agggaagtac gtcgagactg
      961 cggtacggcg aacggcagcg atctggtggg agaaagagga ggaataaggg gacaattccg
     1021 gagggggaaa cggcacccct gccgccatta ccagaacgac cctcacttac ttttctgcct
     1081 cacgaatgag ctgattgatg atcgcttcgg agatgtcgcc ggagtattca cccgcccgcc
     1141 gtatgctttc tgcgatatac ccgatcgaca gggcaaggtc accttttttc tggtgtgcga
     1201 gagcgctgat gtcatcagtc atcttctcaa ggtcggcgac catatcgatg gtgcgattgg
     1261 cgtcgacgat atccgatgag aagagagccc gcatactgga atttaagata ttcatggcat
     1321 tctttgtagc acccttcata tcggcgatga gccccggatc aacctcatgg tcgacgagca
     1381 tgggaacgtg agaggctatt cgaacgccat ggtccccgat ccgttcgagg gtcctgctta
     1441 caatatagta attcgaagcc tcctcaacgg atacatgcag tttctccggg aggcttgcat
     1501 ccctgaacat gaggtgatac tgccgtgaga ttagccactg gagtttatcc acttcggtat
     1561 cccgattgac aacgtcttcc gccaggctct tatccttcgt ttcgagggca accatcgcat
     1621 cgtgaaacat cgtcccgata atgacgaaca tccgtttcac gctattctca aacggcatct
     1681 ccgatgggtt cagaatatcc ttgatgagga tgagagaatc agcttcctcg acgatctgct
     1741 ggccgatggt catcgaactg aaatcgcgga cggccttccg gattgcaggg ggtagtttgg
     1801 tagaagagga tatacgaatg gttgtatagc cggcgatgta cgaccccaca agacatcgga
     1861 agagaaaaac gggatttcct attgcatcgg cactgaattc ctttattcgg gactcttttt
     1921 cctccgagga gcggggaagg atgagaaggc ttccatcggt ctggatcttg atctgaaggg
     1981 gttcgttttt cttgatattc atggaccgaa cccattcttt gggtaaggag atgacatacg
     2041 aagacccccc ggtcatctgg accttcctga aatccataga gagtattctc tatgaaagta
     2101 gatgaaactg atcatttttc ccaattaaat atatatcacc atttctttta tgaggccggc
     2161 catattcaat gtcgaaaatc aataaactta ttcgtatctt tcatctatgc aatgacaaga
     2221 aagagcctgt cagggagttc aaggagtatg accacggttg ccgttgccgg cattctcgtg
     2281 gttcttctga tggtggcggg atgcgttggc aatcaaccat ccaattcttc ggcatccccg
     2341 tcggagcaga tcacggtaac gggatcgacc acggtgctgc ccatcgccga gatggagaag
     2401 gaggcattcg aagcgacaca cgcctccgcc gagatcatgg tgagcggagg gggctcgagc
     2461 gtcgggatca aagccgtcgg cgagggcacc gcggacatcg gcatgacctc acgggacctg
     2521 aaggccgagg aggtcgcagg ttaccccgat ctcgtcaaac accacatcgc cgatgatgcg
     2581 atccttctga tcgtcaatcc gcagaacacc atcgatcgtc ttacgctcga ccaggtgcgg
     2641 ggcatctaca acggcaccta cacgacctgg tcccaggtcg gcgggtccga tcagccgatc
     2701 gtcgttgtgg ggagagacag cgcatcggga acgcgggagt tcttctccga gttcgtgctc
     2761 gcgaaagagg acttcaccaa acagcaggag gagttcaact cgaacggcgg gattcagcag
     2821 aaggtatccc agacccccgg tgcgatcggc tacgtcggcc tcggctacac cgacggcgtg
     2881 aaggtgctgc gactggacgt cggcggaact gccgtcgagc ccacgattga gaatatcaca
     2941 gacgggacat acccgatcag ccgaccgctt tatatgctga cgataggaga gccggacggt
     3001 cttgcgcggc agtacctcga tttcatcatg agtgcggaag gacaggagat cgtggcaagc
     3061 gccgattata tcccggtaca ggagtgaccg ggtcttctct tctcttttga tcgtccggag
     3121 cgctacaaca gcagagtgat aatgaaaaaa gatcacggta caccggctca attattccag
     3181 agtcccgagt ggctccgaag acccggcatc aggatccctt cgaagaccga cctcccctct
     3241 gccgggatga acaggcgatc tgtcgacctg gttatcggca gattactttt ctttgtcgca
     3301 ctcttcgcaa tcatcaccgt tctgttcatc atcgtcttcc tgctccggga cgggtaccag
     3361 atcttccttg agatcggcgt atgggacttc ttaaccggca cggactggaa cccggccggg
     3421 cagcacccgg cctacgggac acttccgctc atcgtcggta cgatgctggt gacggcgctc
     3481 gcgatgatca tcgccgtccc gctcagcatc ggcagtgccg tctacatcgc ggagatcgcc
     3541 ggtccccgga cgaaagcggt catcaagccc gccgtcgagc tgctggccgg catcccctcc
     3601 gtcgtgtacg ggttcttcgg cctgatcctc ctgaccgact ggatacgcat cgccttcgac
     3661 cagccctcag ggtccagctg gcttgcaggc tcgattctcc tcgcgatcat ggccataccg
     3721 acgatcacga gcgtcgcaga ggacgccatc agttcggtcc cccgggagtt caaggagggc
     3781 agtcttgctc tcggagcaaa ccactggcag acagtccgga gcgttatcgt gcccgctgca
     3841 ctctccggca tcagcgcggc gatcatcctc gggatgggac gcgccatagg cgagacgatg
     3901 gcggtgctca tggtcacggg gaatgccgcg atcatcccgg atccaatcat caacgtcttc
     3961 tctccagtgc ggacgctgac cggaacgctg ggcatcgaga tgggcgaagt cgcgttcggg
     4021 agcccgcact accatgcgct cttcggcgtc gcggtggtcc tgctcttcat cacgctcgcc
     4081 atcaacagcg cggcaagggt catcatcaac cggatacagg ggacgcaggg tatgacacgg
     4141 cccgcaccgt ccggaacccc gggtacatcg accggtcggc tgaaggccgc gctccggcgc
     4201 accgcaccgt atcgggcgtt cagagcggcc ttccgacgca tcgcccgcgt cgcatcggcg
     4261 ccatccggaa gggttgtcca ccccaagact gcacagcgat gcgcgttctt cctgatctcg
     4321 ctctcggtca tgaccgtgct cgtgatcctc ggcgtgatcc tcggcgagat cgtcgtcaat
     4381 ggagccggag cgctcacctg ggagttcctg accgggtccc cgaaagacct cgggagagca
     4441 ggagggatct ttcccgcgat cgtcgggacg ctctatctcg taggaggggc cctggcagtg
     4501 gctcttcccc tgggcattgc gacggccatc tatcttatcg aatacacggt ggacaccccg
     4561 ttgacacggg gcatccgtgc agcggtggac ctgttgaacg gaacgccgtc cattgtcttc
     4621 gggctgttcg gcttcgcgtt ctttgtcctc ttcttaaact tcggcgtctc gctgatcgcc
     4681 ggacagatca ccctcggcct gatgatcctg ccgacgatca tacggacgac cgaagaggcg
     4741 ctccggtcga tcccccatgc tctccgggaa gggagttacg ctctcggggc cacgaagtgg
     4801 cagacgatct ggcaggtggt cctccccccg gcgctccccg ggatcctcac cggagcgatc
     4861 ctctccatcg ggcgtgctgc aggcgagacg gcccccatca tgttcaccgc ggtggtcttc
     4921 agcagcagat tcctgccctc atcactctcg gagccggtga tggcgctccc ctaccacctg
     4981 ttcattctta ccacaaacgt tccaggggca tccacgcaga agttcggaac cgccctcgtg
     5041 ctcctcctca tggtcctctc gatctacctc gtcgcgatcc tcgttcggcg gcgatgcgac
     5101 cgggcaaagg tgatgtaaat ggacgaatcc acaattcttg aaacaaagaa cctcaacctc
     5161 tggtacgggc agaagcacgc cctcatcgac atctccgttc gcatcccgaa gaacaggatc
     5221 acggccctca tcgggccttc gggatgcggg aaatcgaccc ttctccggtg ctacaaccgg
     5281 atgaacgacc tcatcgactc gtcgaggatt accggagaga tcctcttcaa cgggaaggat
     5341 atccacgacc cccgtacgga tgtatacacg atccgcgaaa agatcggcat ggtcttccag
     5401 aaacccaacc cgttcccaaa gagcatctac gacaacgtcg cttacggccc ccggattcac
     5461 ggggttagcg agaagaaggt gctcgaccgt atcgtcgagg atagcctcaa aggcgctgca
     5521 ctctgggaag aagtgaagga caggctccac gagcccgccc tgagcctctc aggcgggcag
     5581 cagcagaggc tctgcatcgc gcgatgcctt gcggtcgaac ccgaagtcat cctgatggac
     5641 gaaccctgct ccgcccttga tcccatcgct acggcaaaga tcgaggatct tatcatccag
     5701 atcgcccagg accacaccgt cgtcatcgtc acgcacaaca tgcagcaggc ggcgcgggta
     5761 agcgaatata ccgggttcat gtacctgggg aagttgatcg agtttgataa aaccacgcgg
     5821 atcttcgagg atcccggcga agaactgaca gagaactaca tcaccggacg gttcgggtag
     5881 gaagaccatg gtagagaaat ttcatacgga acttgcatcc ctgaaaggag atgtcctgga
     5941 gatgggctat ctctcacgga acatgctgaa tcagtcgctg gaagcactgg agagccagga
     6001 tgtggcgcta gccggatcaa tcgaccggga taaaacgaga ctcttggatc tggaccacga
     6061 tatcgagcag gaatgcctga ggctcatcgc cctctatcag ccgatggcaa aagacctgcg
     6121 gacgattgcg gcctcgatga aggtggtaac gagcctgtac cgcatcgggc gctacggcaa
     6181 agacatcgcc atcgttgtac ccgaccttgc cgacgccccg cacgtaggta acctcgtcag
     6241 cattccccac atgggggatc tcgtcggcgg gatgatcgcg gatgcgctga ttgccttcga
     6301 acgcgaggac ctcgccgcga taagcgatat caaggaccgg gaggacgccg tcgacgccct
     6361 cagatactcc atattccgcg agtgcctcac ctacatgatg gaagacccaa agaacatcac
     6421 gaggtgcacg aattacgtca tgatcgcccg gtacctcgaa cgatgcggag accatgcgtg
     6481 taagatcgcg gagaaggtgc actacatggt gacgggcgat cggatcgaga tccggtgaac
     6541 cgctcatgga agagccgggg tccggggagg tgggctgacg agtatcatcc gccccggcgg
     6601 gagccaacag gggccattat ttatccctgc aaacgaatca tcacagagat gcagaaccag
     6661 cagcccgtcg acctgaacgc catcgcatgg gcggcgatgg atcagtacgg cttcatcccc
     6721 gggttcccgc cgtccgtcct ccgggaagtc ggcgctctcg ccgcgaaagt ctttccggat
     6781 accctcgatg acccccgcga cctccggtcg ctcctctggt cgtcgatcga caaccacgat
     6841 tcaagggacc tcgaccagat cgaggtctgc gaagaaggac cgaacgggga gatccgggtc
     6901 agggttgcaa tcgccgacgt cgacgtctac gtccagaaag agtcggagac ggaccggcat
     6961 gccggccata acgggacctc ggtctacacc ggcgttacga ccttcccgat gctgcccgat
     7021 cgcctctcgg cgggcatcac ctcgctcctt cccggccagg accggatggc ggtcgtcatc
     7081 gagtacaccg tcctccccga tgggagcgtg aagcccggcg atatctaccg ggcaatcgtc
     7141 gcgaaccggg caaaactcgt ctacgaggag gtcggcgact ggctggaggg gaccgggccc
     7201 atccccggga cggtcacgga gacacccggc ctcctggagc aggtcctcct ccagaacgag
     7261 gccgccatgc gcatgaagaa gcgccggaca gagcagggag cgctcgccct cgagacgata
     7321 gaagccgcgc cggtcgtgac ggacggccag gtcaggggtc tcgtcatcca gcagcagaac
     7381 cgcgcgcgat gcctgatcga ggagttcatg gtcgcggcca acggcagcgt gacggcgttc
     7441 ttaagcgacg ccgatctgcc catgattcac cgggtcgtcc gcacaccgaa gaactgggag
     7501 gggattgttg cggaggcagc ggaacgcggc gaagcccttc cccccgaacc ggacgcggag
     7561 gcactcacac ggttcctcat ccggcagaag gcggccgacc ccgaccgctt ccccgacctc
     7621 tctctcacgg tggtgaagct catgggggcg ggggagtacg tggcgttccg gccgggcgac
     7681 gtgccgatcg gccacttcgc cctcgccgtc accgactaca cccacggcac tgccccgaac
     7741 cggcgctacg tcgatctcat catccagcgg ctgataaagt cggtcgtcga cgccgagcgc
     7801 tatcccccct acacgcctct cgaactcgac gacctcgccg tccgactgac cgaccgggag
     7861 aaagcctccc agaaggtcga gcgcttcgtc cggaaggcgg cggccgcagt cctcctccgg
     7921 gagaggatcg gcgactcgtt cgaggcgttc gtcaccgggg catcggagaa ggggacgtat
     7981 gttcggctga tcgacccgcc ggccgaagga agggtcatgc agggcgagca cggtctcagg
     8041 gtaggccaga gagtgcgtgt ccgcctgatg gcgacggacc cgtacaaagg cttcatcgac
     8101 ttcgcgcgta cccggtgagg gaggggggct cccgcccggt tcagtaaccg agctgctccc
     8161 ggatcaggac cagggttatc ctcccctcgg taacttcgtt ctcgaggatc acgtacttgc
     8221 cgatataact cccggtgccg taggcatgct gcgcccgctg gttctcgagc ggcaggcagt
     8281 actcccggca ccgccggagc gcgtcctcaa gcgtcggcac gatcttgttc gtcccggaga
     8341 cgaggatgag gtgagccgcc gcgtggggcc acgcccccat ccggctcccg gtcttgtcgc
     8401 acccgacaac ctcgcccgat tcggcgatcg cctggacgcc agagaggaag tagtcggcgg
     8461 tcacgctttt gcggcggagc gcgtgccgct tctcctcatc gttctcggcg gtgatgaccg
     8521 cgtggtagtc ccgccatccc ttccggttct ccctgagcac ccggtcaaac cccatctcta
     8581 tcagggtcgt cgagtaaccg ttcatgacct cggcaccttc gggcagcagg tcgacgacgg
     8641 tccggagcgc atcctctgcc gtatccgcga ggatcaccct gatgttccgc gcctcgatcg
     8701 ccgcaaccgt cctctcgatc gtcgcgtcgt ccggaatccg gctccatcgt tctctgtcca
     8761 ccccggcatc ggccatcagg ttgattgcgc tgtactcggt atcttttgcc atctgctggg
     8821 taacattcgc catctgatca ccccgggcac aggagaacgc gatcccacat atacctctcc
     8881 atagaccggg catccgcacg ggggttgagg cctccacggt tgctccgggc cgggagcact
     8941 ggtgacaccg tttcctttgc acaggagaat tccggcgtgt ttttttggtc ctccgcccca
     9001 cgaaagggta tgaagagatg gtatctcgtc ggcatagtct gtcttctggt cctcgcggcc
     9061 gggtgcagca gccccggaga ccgggaggtc gtggaggaga atataaccgg gggagacctt
     9121 gcggacctgg tccgggacgc cgccgcgtat gcggagactg caggaagaga tgcagccctc
     9181 gccgagttcg gcaacgagac cggccggttc tcgcagggag ccctgtatgt ctacgcttac
     9241 gactacaacg ggacactcct cgcccacccc tataagagag aggcggtcgg aacgaaccgg
     9301 gagaactgga ccgatgtccg gggactgccg gtgacccgca taggtgcccg gatggcagca
     9361 gacggcgggg gcttcatcac ctacctctac ccggtaccgg agaacaggtc catcgatgag
     9421 tcggcagagg actcctatgt cccgaagctc ggctacgtcg cccccgtcgg ggatgcgtgg
     9481 tggatcggat cgggtgtcta cctctccgat gcgaccggtc ccggccggga gccgtatccg
     9541 gaacccatat cggcgatggt cgacctcgtc gaacgcggtg ccgcctatgg ccgcgagcat
     9601 ggagcagaag cggcgtcctc tgagatctca aatagatcgg ggatgtttgt cgatccggcg
     9661 gaacactacc tctacgccta cgactacaac ggaacgctcc tcgcccatcc ccacctgcag
     9721 gccgcgatag gcgcaagcct gatcgagagg caggacccct tcggaatgaa gaacatccgg
     9781 gcgctcaggg acacagcaca gtcgggaggt gggttcatcg tcttcgtctg gccaaacccc
     9841 gagcaggaga accgcgagga actgaagatc gggtacgtcc gcccggtcga cgacacctgg
     9901 tgggtcggct cgggggtcta tctcagcgag atcaccgggg aggatgcatc gttctcctct
     9961 ctgggtccct gaaaaatctt ttttcccaaa atttgcattc tctccccggc agatcattaa
    10021 tgcatcccca aaagccggga tcttaccccc ggggacatct accgggtaaa acgtctggag
    10081 aagtcctcat ccgaccgtca ggcggatagc acggccttgt ttctgaaaaa aaagtcagga
    10141 ggtcggtgcg gcctccatgg ccgtctcttc cgcccccacc tcctcgctgt cccagacgag
    10201 ggtgctcttc atctcggtca tgatcacccg tgaaccggag gggacccgga tcgcgtcgcc
    10261 gatatcctca ggcgcccgcg cccagacgac ctggtgcttg acctcgccgc cgggcagcag
    10321 caccacgacg ctgtaggtgt tcataccctc ctcaagggct ccctcactct ctcccggggt
    10381 aacacgatag cacgtaaatg tgaccggttc cgatccttcg ctcatactct gtcctgcttc
    10441 acctttcata ctaatcctcc gtcacccgga cgtgcgcggg ctctcgcggc tctgaacggc
    10501 ctccacaaag agcgggaggt tgccttctcc gaccgccggg gttcccgcct cactccggag
    10561 agccaccccc ttctggatcc cctcctaata ataacttgcc catgccggag ttcccgggag
    10621 gggcacccca cacccgggcc gggaccctcc tccagtgctg cccggatggc taccggaaat
    10681 catgcccgga tgaggaccat gcgcgacgga taacgattcc gtccggcgac ggcacgggaa
    10741 tgccgggtcg tcaacccgcc ccatccaacc ttccggagct gtcccacaat atcatcgggg
    10801 ctcccgggat agcggcacca acctccttgt cccggagccc ggccacctcc gccagcaccg
    10861 agaggccgag ccgggcattc ttctttgtat actcaaacga gacatggtcg atggtgtcgt
    10921 ccggggtgtg ggccgggccg tgctcctcgc agtgggtcgt cacggccggg agcccccggg
    10981 cctggaaggg aacgtggtct gaggcgcagg tgtggttgtt cgcggtcagg gtgaagttcg
    11041 tggcgtagag gctgttgtag tctgccatga gtgccgcgag atcgcgagag cgctcgtcgt
    11101 agacgatgtc gaggatgaaa cggttctccg ggtcgtaaca ggccgagtcg tagttgaggt
    11161 agaacgcgac agggttcgtc gcattcctgg catagtcggc gctgccgtac cgaccatcct
    11221 cttcggcgtt ccagagcgca aactgcaccg gccggtcaaa ctggtggcgg ctcatcaccc
    11281 gggcgagttc caggacgatc gcgacgccgc atccgttatc ggtagcccct ggcgcgtcgg
    11341 tagggtctga ggacctgctg tcgtagtgcg cccccaccac cacgacctca tcggaggtat
    11401 tgtcgctccc ggggagcgtg gcgatgacgt tgttgagttc gccgccctgg aactccacct
    11461 cgagcccggg gatgctggca aacctcgcaa agagatactc acccgcctcc cggtttccat
    11521 cggtcccaaa gacccgggtc gaaaagttct gcaggtcgta tgtcgtgttc cagagttcgg
    11581 tttcgttgat ctctgcaagc attgtggcga tggccgggtt gtactcccgc tcttccccgg
    11641 atatgccggt ggagcctgcc ggggcagcta cgaggagaac tcctatgccg gcaagcagca
    11701 aggcacagag cagcaccctc cggatgcagg catgccgtcg ggggcacctg tcgcgtgctg
    11761 aatcacgtct catgaatgat cacccagaag acgccgcata gggcgcagag atacatatac
    11821 tttggctccg gatagccgga gatccatgat ccggaccgtt tcctgctcga gtcaccggaa
    11881 taacgccctg gctcccgcca gcggggacga gcctccgggg gccagatata tatcgcatgg
    11941 cagagagtag cctttagacc caaccgggat cgccaccgga gcagacatgc ccgaccgacc
    12001 atcagcaggc gacgagtcga ccctcttacg gtggatgcgg cttgagatcg gcaggatcaa
    12061 cgacggtgtc gttgcggagc ggagacgcct ctcggaactc ttacgggagg aacgcccgtc
    12121 gtccgtcacg aaaggcggga accggtacga cttcgaccgc caggtcctcc tccggctgga
    12181 gcaggccctc ccgggggagt ggcagagacg gctccggctt ccgatcctct tctacttcga
    12241 ctccacggtc ccggacagtt tcttcctcgc ggatgaagct gcagtacggg ctctccagtg
    12301 cctggaagag atcagccctc tccggaggat gcagggcggg aggctctgga tagcgaaacc
    12361 tctcgtgtac gacatcatga accgataccc gacggccatg cagatcatga tgggttaggg
    12421 gatgacccta caccctaaaa gagatgagtt cttcgccggt gcccggtgag gtactcaccg
    12481 gcatggcctc cggccggtgg ccgaaaatgg gatacgcggc cggtgaggcg ctctcgggcc
    12541 gataatccag ggacccggtt ccgccagccg gtgagaccac gggagacggc cgatcctgtg
    12601 ttccgacaac gatacaggcg attaacccgc caaagaccag ggcttgtatg gcgacggtca
    12661 ataccacggc catcttctgt tgatagatct cgatcacctc gcgtgccggt agcggggatc
    12721 gtatttaaat tttgctaatt atatattatt aattattata ttttgttaca aatgtccaat
    12781 aggcatagcg aatagacgga ggccgtgcat ccggcagccc gggactgcat gcacacggtt
    12841 tttgggatag cccgccattc gcctcttttt ggaagcgggc ctaccgggag gaacgacctg
    12901 ccggctgccc gggatcgttc ctcctcgcga tggttcgctg gatccgttcg aagaccgggc
    12961 gggtgacctc atccatctgc ccatcaaagt aatccaccca ttccgcaacg agcgcgagct
    13021 cgccctgctg ccgctcgagt tctccctgca ggtgatcgat ggtcttccgg agacggccga
    13081 cctcctccac cctgtttccc cgttctcgct cgagctcctc agccagacgt tcgatgcggc
    13141 gttcctgccg tgccagcgtc tcctgcataa cccggaggtc gatcaccacc tcgggaggga
    13201 gcgggacagc ggttcggtca accccttctg ccggctcttc aggtgcaggc gatctccgcc
    13261 cggatctgat ctcccgggcg atctcttccg gtgcgaggcc cttcccggag agttctacga
    13321 gttcccggac aacccggacc gctttttgcg ggaagagtct gacagggccg atggtccggc
    13381 aggtaaaaag gctgtcgtaa gtctggatga gtgtccggac atcctgctcg gagagcccgg
    13441 tgaggtgcgc gatatcgccg atcttcagtt cttgctcgct catgatacca tcctctgcac
    13501 gcgctcgagc ggtaaccctg ctgtaactct ttgatctcat caggtatagg tacgtgccgg
    13561 atcacggggt ttttcttgcc gcggtcccag tacggtacaa tgaacagggg agcgaaggag
    13621 agagtcccgg gcggcatgac cgtcgtccgg aaggcggtga ggtctgcccg cctccaggtg
    13681 cggccggatg ggaccctgcg ggtggtcgcc cccccatcat tcgatatcgg ggagttgctg
    13741 cagcgcaacg cggcctggat caaagagcgg cgcgaagaat tcgaccggct ggccgccgaa
    13801 gggtgcgggc acgaggacct gctcctcctg cacgggcggt tccaccgtct ggtccggggt
    13861 gcgcgattct gcatcgacga gacatgcggg gctgtcgcct gcccatcccc tggcgccctc
    13921 cggaggaacc tctcagggtt gttaaaggag gaggtcttaa accggctcga ggcctacccg
    13981 gaccttgcgg gccagaggcg gggacagatc gcggtgaaac tgcaaaagac ccggtggggg
    14041 agttgctcga cgctcggcaa cctgaacgtc aacctgcgcg tcatagccct tccagaaccc
    14101 ctgcgggagt atgtcgtcgt ccacgaggcc gcccaccttc gcgagcagaa ccactcaaag
    14161 cggttctggg acctcgtggg cgaccactac cccgggtacc gggcggcgga ggccgagctg
    14221 cggcgctact gggtcatcct cgaacggaac cgggtctggt cctcactccg ggatacgaag
    14281 tgaggtggta tcaggagtac ttccgccaga acatggagag tttccgcgac tccgcccacc
    14341 gctcgtcgag ctggtcgatg atcccgttga tccgctgcac ctgctgccgg atcttctccg
    14401 tcgctccggc atcgtcaagt agttcggccc gccccatgat cgcctggagc gggttccgga
    14461 tatggtcggc aagcagggca aactgctcca tgttccgctc tatctgagca taggccttcg
    14521 atctcaggtc ttcaacctcc ctctgggcgg tgatgtcccg gccgacggtg acgatcctcg
    14581 cgacctcgcc gtgctcgaag atcgggatcc gcatctcccg gaggatcatg acccggtctc
    14641 cgaacttaag ggaccgctcc gatgtcagcg gccgaccggt ccgtgcgacc cgctcgtaca
    14701 gctgcctcgc ggagggaggg aggaacggca ggacggtgaa gagatccttc ccgacgacgt
    14761 ccgtcgcgag gccgaggtct tcgcaccagg cgatgaaggc gtcgttggcg agaagaaggg
    14821 tgagcccggt atcgacgacg gtgacggtat cggtgatggc atcaaggaca atctggtaga
    14881 ggtcttcgga ctcccgaaag agatactcgg actgctttcg gtcgagtgcg ctcgcgatga
    14941 tatggacggc cgcagagagg agcgttacgt cctcgtccgc gaaccgggcg tcgccggtat
    15001 tccggtcaaa cccgacaaaa ccgacgaccg ccccgttcag ccggagcggg accatcagcg
    15061 tcgcggtagc gtcgtgccgc ttcaggagct cgcgctcctc cctgcccggc cgcccttccc
    15121 gggagatccc gctcacgagt acggtctcgc cctccaggat ctccccgacc caccagggga
    15181 gatcgcccgt gaggaacttc tgggactccc cccgccgggg cttctgatcc ggccggcacc
    15241 actcgtgcgt tgtgccaagg agcgttcggg aatcgttgaa gagggagagg taagccctgc
    15301 ataccccgca tgcacggccg agatcctcga gcgagtcgtc gatagccgga tcgatctctg
    15361 caggatccgt gaaccgtgcg ggcatcgccg cgacggccgc ttcgaggctg cggcggggac
    15421 cgggtgcatc ctccgccttc tgctgcacgg caacctcgat gcaggagagg aggagccagg
    15481 gatcggcggt ctcggggtca cggacagaac tgatcgagac cctgacgtca aaggacgatg
    15541 aatcccttcg ccgtgcggca accgtcccat tccaccggcc agaggagaga gcgcagccga
    15601 tgcagtcctg cccctcgctc cgggagggcc agaggagggt cacgggcttt ccgacaacct
    15661 catgcaggtt atacccccac atggcaagca acgaagcgtt ggcaaaggca atgcgcccct
    15721 cctggtcgat gatgcagatc ccggagagcg aggtttcgat cacctgccgc agcatcgcgg
    15781 cagtaacgcc gggggtcccg gccggggtct cactggcgac agaatcctct ctcccctgat
    15841 tctccaagac gtctccctgc tgctccggag aaatgcctgc cgccgaacgg ttcgctgtgt
    15901 gcagatccat acttgatcgt cccgggcagc agaacagagc tgccctcgct gtcctggaga
    15961 gatcgcgtac tgccgctctc cggactccgg tccgggttgt tccggacggg ttaggatgat
    16021 taatcccccg ggctgcgtat tatagtttct gtttgtgttg cgccatcttc ggcccggagg
    16081 gggtcatccg cccggtttga aacagcaccg gggggatcaa cggggccccc cgacaggaga
    16141 agatcgcctg cgtcagcccg gggcgctcag gcgttcgtct ccgctcgccg cgagtaactc
    16201 cggtacatca ccaggagtgc ggtgccgatc aggaggaaga cggcgagcga cacccagaac
    16261 ccggcccccg ccgggatgag gttcacggcg tagtcgatgc caagagcgat ggcgaaactg
    16321 ccgatgatgc cgccgatggt gatgcaggag aggacctccg tcttcgtcat cccgagcatt
    16381 ctcccgacga tggaaccgac gatgctcccc gatccgtcaa aggggagagc cacgaaagca
    16441 atgaggccga cgaagtagag ccgttcaagc cacggccgcc ggtcgaggaa cgtcctgccc
    16501 gccgccgtga accggctgac ccagggcccg atgtagggga tgcgcaggac cagcggaaag
    16561 ttccaggcca tgaagagccc gccggcgaag tccaggaatg ccagggcaag cgcgaccagc
    16621 caccagggaa tgccgcaaag gatgcagacc gggatgatcg tctcccggcc gagcggcgga
    16681 acgatgtagg cgaccatcat cccgacgagc aggagccact gctccgccgg aaggaagaac
    16741 catactacgg caaaaaagag cccgcagagc aggggcggaa ccgagacctt cagccagctc
    16801 gcccggtcgt caaccccgtc accgccccat acaccaatct gtcgcatgaa ccgccatcac
    16861 ctgcgggtga gtgccacgcc cggggccgga gtcactcctc accggggggc ccgggggggg
    16921 ccggggggac cgggataccc cgggtgttca ggatgcgctc gacgatcttt gatgaactgg
    16981 caagcgagcc gggatacccg ggtatccgga cgacgtccgc gtcgagcccg cggtcccgga
    17041 gtctctgccg gagacgctcc tcgtcgaaat gctggttaaa cccgagcgtg atgatgtcgg
    17101 gccgtatctc cgcgatcggc ctgaacatat cgtggaggtc gccgaggagc gcatggtcga
    17161 ccggccgtaa tgcccggacc atccggagcc gttgatcttc cgggaggata ggcctcggct
    17221 tatgcttcac gttcgcgtcc cgcgccacga tcacggagag ttcgtcgcca agtttccggg
    17281 actcctcgag gtagtagagg tgcccggggt ggaggatatc gaacgtcccc gtcgcgacca
    17341 cgcggatcac tcgaccacct caaggtttgc gggactgccg tccgcccggt agcaccgcca
    17401 gtccctctct gtataggggt acccgacgat gatgtggtac cgaccgaccg aggggaagaa
    17461 cctgaggtcg gctgacgacg gggagaggac gccgttcggg tggctatggg cgctgccggc
    17521 ctggtgggta tccagcggga gcatatcgat gaagaacgag gcgctctctt ccccgacgat
    17581 ggtgccgggc accaggtcga ggtcccggat aacgccgtcg cgctcccgca acacggcaac
    17641 gaactcatcc gggtggtgtt cccgcccgag ttcgaagagc atcgcaagca gatccctgct
    17701 gatcccgcat atggccattc tactgaatag tgggggagga ggaaacataa gccctcgcgt
    17761 tcggcatgcg tgcccggccg catgcaccgg accaccggga gtcgggagaa aaaccacatg
    17821 atgcccgtgg ggccacaggc ataccggatg aaaatatgta ggtgacccat tttcatacga
    17881 aaatggggtc gtttaaggga tgacccttcc ttccggcagg gcctcggagg ttatgttccc
    17941 ggtcacattc tcaaccgggg ttatgttctc aaccggggtc acgttcccgt cggcggtgac
    18001 gttcaccgtc tcgaatacga gcggtatgag cggcgtgtgg tcgttattga ccagctggac
    18061 cgagaggttg tgctcgcccg ccgtcacgtt ctcccaggtg taggaggtgt ttgcagagac
    18121 aatgtagccg ccggtctccg ggatggcggg cgcactcgcg ttcgtcggca ccggggcatc
    18181 caggtagtag tgcaggtggc cttcgcccgg ggcgttcggc tgtccggcag gctcgatccg
    18241 tgtgaaattc gtgacattca cgctcaccgt gacgttcccg gcagtgacat tggcgccnnt
    18301 agtgacgttc gcctcagcag tcgaagtcac gttctccggc agcacaagac ccggcggcac
    18361 gagaaccgtg tcgatgatgt ggacgatgcc gttgtcggcc gtgatgtcgg atgcgacgat
    18421 ggtagcgttc tctaccgtga ggttgccgtc cgccatcccg acagtaaggt tgtcccccgt
    18481 aagtgtctca agaaccgtct tgttatcggt ggcgttcgcc tccgccatca ggtcttctgc
    18541 ggtgtagttc cctctcacca ggtgatgcat gagcagggca tcaagcgtct ccgtgtcgtt
    18601 cgagagtccg gcaagggtct cgttgccgag agcctcgaag gcggcattgt ccggagcaaa
    18661 gaccgtatac ggtccccccg tagtaagcac ggtttcggta aggcctgcct ccccgacggc
    18721 atccgcaaag gcagtcaggt tttcatccgc cgcgaccagg tcgatcagcg tcatgttcgc
    18781 cggctcctcc ccgggggtcg cgttctcgtc cccgtcgacc atcatcccct ccgggacgac
    18841 gatgacgacg ccctgcatgg gcgtccgctg cgtcgaggcg ttgagcgtac agccgtacgg
    18901 gtaggtgccc gtatcggcga acgtgtagtt gaacgtcccg ttctgctcta tcggcccgga
    18961 gtcgaagagc cctcccgtgc tggtgacagt gtgactcacc tcatcataat tcgtccaggt
    19021 taccgttgta ttgccctcga ccgtgagcga ggcggggtgg tactccccgc cgcgtatatc
    19081 aacgtcggca gttaccgcga gagccggcag ggcaacgaga cctatggcca gggctgctac
    19141 gagcccgagt atggttggat tccttctcat gcagtttcac cacgcatatc taccctggca
    19201 gggagagcgg ccggcatcct gaatgcagac cctgagcaca cttctgatcc ccgaaggtcc
    19261 acggtagcgg gaatacccgt ccgaacagtt cggcaccgct cgccgctgcc acggaggggg
    19321 aaataaccgc acaactatat aaccattatt atatttcaaa aggttgaata tccgaaccgg
    19381 gttctgcgcc ctccatcgag agaatctacg ggagcggact gttcagaacg gcccgtcctg
    19441 ctcgaaactc ctcctgtatc ggatggcaag ttccgccttg aagagttcct ttccgaggta
    19501 ggcggcatgg tcgaaccgcg agaggctccc ttcctc
//
LOCUS       DAJB01000267            5994 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  TPA_asm: Methanoculleus marisnigri isolate UBA478
            UBA478_contig_73242, whole genome shotgun sequence.
ACCESSION   DAJB01000267 DAJB01000000
VERSION     DAJB01000267.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027610
            Sequence Read Archive: SRR5037141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Methanoculleus marisnigri (hydrocarbon metagenome)
  ORGANISM  Methanoculleus marisnigri
            Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
            Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 25.90x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Methanoculleus marisnigri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA478_contig_73242"
                     /isolate="UBA478"
                     /isolation_source="waste water"
                     /db_xref="taxon:2198"
                     /environmental_sample
                     /country="Canada: Medicine Hat Glauconitic field"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327522;
                     metagenomic"
BASE COUNT         1145 a         1748 c         1764 g         1337 t
ORIGIN      
        1 atttactgtc ggtccctgca tcatccatct tgccttgaaa gcctggtaga tattcgtcaa
       61 ctggtgtatg gggtcgtcct cgcatatatc tattgcgtcg ccctccggag cgcatatccg
      121 ggggaaaaaa gcaggttttg ggttattcga tgttctgcat ggtctgtgcc gtcccggtat
      181 cagaaccagg catccagtgt cttctgcgtc gccttcaccg agaccttggc gagagcgctt
      241 tcgacccgct ctctggagaa gtcgtagcgc ccgcagagca tctccacgac gccctcgacg
      301 tccggcgacc tccactcgag ggtgtagtcc ccggtgaccg gcgggttaag gaagaagtcc
      361 cggattggag cggggtcgaa atcgggctgc ttctccgcga tcacggactc aaactcgccg
      421 tttcgcacga ccttcaaggc tgtcttgccg ccgatgcccc ggatgcccgg gttgaagtcc
      481 gtgcccacca ggatgccgat ctcgacgagc tgctccctcg tgacgtcgag gcagtcgagg
      541 agggaggaga ggacgatccg ctcggggttc acggtgatcg tccgccctcg catctttcgc
      601 cggccgctga ccgtgaggtt gcgcaccagc accggggagc cgaagaggag cgagtcgtag
      661 tcctgcgaga ccgcataggt gaccatcccc ctctgtgcca tgtgggctgc ctgcgcttcg
      721 ccttcgctcg gtgcctgcac ccaggggatg ccgagcaggt cgaggagttc gtgagacgac
      781 gcaatgatat ggctgtcgat acgggtggag gcgctcgcct gcttatacgc ctccttcata
      841 tccccctctc tgagcgccgt tttccaggct tcgtcggccc tggtacggac gagacgccgc
      901 tcgtcgatcg tctcctgttt gaactccggc ggcttcccgt cgaagacaaa gaccggcttg
      961 atgccctttt ccaggaagtt gatggaccgg aagaggatgc cggagaggtg cgaggtgacc
     1021 cggcccgcgc cgttcatcag cggggtgccg tcgggctgcc ggatgatgga gaggaattga
     1081 tagagcgcgt tatgggcgtc cactgcggcg gtgccggaga ggccgtccca cgtcaacgtc
     1141 tctttacagt ctgccaggat atctcggata gcaacgccca tgctacctga tcatgagagg
     1201 gggagaggcc attaagatgg cggtccggtt caccggtaca tccgccggga gagcccgtcg
     1261 acgatctcct cgatgtgact gaccgtattg tcgtatttgg tgctcattgt ctttcccatc
     1321 tcctccatgt tcacgcgcat ggtgcgctca cgttgtgcga cgctcttatc gaggctcaca
     1381 atcttcatac caagagagat gagcagcccc ccgagggaga gcatcatgaa cgtcgcagca
     1441 accgctataa tcaggtcctg ccagaaccgc atcaccagaa cgatggtgga gactaccatc
     1501 acgatggtga gaacaatgtc gccgatgagt tcgcgaatct ccatatttta aaatgatgag
     1561 gatgcattaa ttaacctacc taaaatgaat ggagttttgt gatgcagata cgcgactggc
     1621 tgccacttct tggaatgccc ctcatgctct tatccgtcca gctgatcgcc ctcgttctcg
     1681 ttctccccat gcaggcggcg ggtcttgtgg cgtttgaaga ccccgagtcg gtcgtaaatc
     1741 ccctgatatt catcgggatg ctgcttgcct ttacgctggt cctgctcctc cttatccggg
     1801 cgggagggcg gcgtttcatc tcgatcatca tcggcatctc catattcctg acgttcctct
     1861 acatcttttc agcgctctcc aacctcgcgc tcgggaccac cgatgccgct gtcatcggga
     1921 cgctcatcgg ttccggagcc gcgacggcgc tcctctacct ctacccggag tggtacgtca
     1981 tcgatctcct cggcgtgctg atatccgcgg gtgtcgcatc tatcttcggg atatccctcg
     2041 ccatcctgcc ggtgcttgtg ctgctcgtgc tgctcgctgt ctacgacgca atatcggttt
     2101 atcggacaaa acacatgatt acgcttgccg aaggcgtcct cgaaacaaaa gctcccatca
     2161 tggtcgtggt cccgaagaga atggactact cgttccggcg ggagggtctc aacatagggg
     2221 agggggagga gcgcggcgcg ttcgtcatgg gcatgggcga cctgatcatg ccctcgatcc
     2281 tcgtggcatc gtcgcacgtc tttctggacg cgcccgctgt cctctggatc ctctccgcac
     2341 cgacactcgg tgcgatggca gggtcgcttg ccggcctcgc cgtactcctc ttctttgtca
     2401 acaagggaaa gccccaggcg gggctccctc ccttaaacgg cggggctatt cttgggtttc
     2461 tggttggggc ggctctcgca ggttcgtttt cgtggcttcc tttttgtgtg tgagcaggag
     2521 gccgaagacc gcctccaccc cttcgccggt gagcgtggac atctccgggt aaccctcgat
     2581 cccctggata tccgccttgt tcgcaaccgt caccaccggt acgtcgacga tccggtgtat
     2641 ctcatcgagg agccggagct ggtcgtcgag cgtgtacccg cagtgctcgc tggggtccag
     2701 gatgaagagc acgacatcgg cggcgttgat gatggcgctt accgcctgcc gctcgatggg
     2761 gttcctctct tcggccgggc gttcgaggac tccgggcgta tcgacgagct ggacccggtc
     2821 gcgtttgccg atctcgtggt ggccgacgat gatccccttg gtggtgaacg ggtaggcggc
     2881 gatctcgggt tctgccgttg agacaagccg gataaacgaa gatttcccga cattcgggaa
     2941 tcccgccacc accacggtga agtcgtcgtc gttcacgtgc gggagtttcc gcaggatgtt
     3001 gcgggcctcg ttcaagtata cgagatcgtc ttccacctgg tgaacgatgg aggctatgcg
     3061 ggcaacggcc tgtcgccgtt ttgaggctgt gtccactccg gatcgcatac tccgggtgta
     3121 atcggagccg atgatccaga cctggtcggc cgcccagttg acggcgccga gagatcgttt
     3181 cagccggtcg agcgagacga ggatgtcggt cacctcctgg tagaagggtg agaggcgctc
     3241 gaagctcgga aacgagctga ccacgctttt caacttgtcg tggatggctt tcgcgaccgc
     3301 tctcacgaac tcttcgtttg cgctatcgac gttggtcttc agcttccttt tggcagctgc
     3361 tctgcgaaga ctgcggtcaa gcacttcgtc tgccgtcgga acggtcgaga tggtttcaaa
     3421 ttccactgta cgcacaacct aatgtattta aacaacgtat aatgactgat aattatggag
     3481 ctctctccca ttcagaagga tatcctgata actctgatta ctctatatca tcagtcctcc
     3541 cactctataa aaggtgagga gatcgcggat gtgctcaagc gcaaccccgg cacagtccgt
     3601 aaccagatgc aggcgttaaa ggcgctcggg cttgtcgatg gcgttcccgg gccgaaaggg
     3661 ggctatagcc cgaccgcagg tgcatacaag gaactgaatc tcggagacct cgagcaccag
     3721 tccgaggtgc cgatcttccg cgatggtgag agagtgaagg gcgtccgggt cgccgaactc
     3781 ggtttcacca ctctctgcca cccggacctc tgtcaggcga cggtcaggat catcgggagc
     3841 gtaaagctct tccgcgtggg ggatatggtc ttgatcgggc ccacgccggt gaataaactc
     3901 ctcgtccgcg gtgagatctt cgggatggac gagaaccttc aggcgctcct gatcagcgtt
     3961 tcagagatga catccctgcc gaaacagtcg atcaagcact acatgagcac cccgctcctg
     4021 accctgtcgc tctccgccac cctccgcgat gccgttcacc tcttcaccat gcaccatatt
     4081 cacggtgccc cggtggtgga ggcgaacggc gacctcgcgg gcatcgtgac gttgagcgat
     4141 gttgcccgcg gcctggacgg gggcatgacg ctggatgcgc ctgtcgcaga gatcatgacc
     4201 gccgatgtgg tggaggcacc gtcttcgatc cggctctacg agcttgtcgg ccggttcaag
     4261 gaacgggaga tcgggagact ggttgtggtc gagggcggca agcccgtcgg gatcgttacc
     4321 cagaccgata taattcgggt ctttccctca ctttgatccc ctccccgaag ggaatttcgc
     4381 gcttcaaccc ctctggaatc agataagtat ttatatgaga atacctatgg tgcttctgta
     4441 aaacgagttc tgtgtgtgag tgcatttcac agaactcaat gaaaaggggg aaatgagatc
     4501 atgactgata tacctctggc accagttggc agaattgtaa agaagtccgg cgcggaacga
     4561 gtgagttccg atgccaatga agaactcgca aaactgatgg aacagtacgc gtcacggatc
     4621 gcgaaagagg cgatcaagct ggcgggccat gcgggcagaa agacggtcaa ggcaactgat
     4681 gtcaggatgg cagccgagac cgtgagataa atcaacccta cttcttattt ttaccgtcag
     4741 gtgagtggtc gcccgtctaa aggatctccg gctccttgtg tgagacgagg cctgaggcgt
     4801 ttctgtgagt aagacctgcg gcacgccagc atcgcccggt aaagggttct ggctatcggg
     4861 aacaatttag tcccgaggac ccctctgcag cccccgacaa cggcagtggc accctcccct
     4921 ggtgaaggat tctataatgc cgctttcccg gggttttatg ggccttagaa catttaaata
     4981 gtacaaacgc cccacggttc tgtatgagca aaacgttctg ccgtgccctt gcgaggaaga
     5041 aagtgatgag taatgatggg atgctcatcg ggactatcaa gaatatcatg gtcgacctca
     5101 acaccggaca ggttgtcgac ctgctcgtca aaccggacga gacgttcagg actgagggct
     5161 acaggacaga tggggacaag atgctcgttc cgttcgaggc ggtgaaggac ataaaagact
     5221 atgtcgtggt cgatcgctac ctgctgaaga gatccggata atactttttt accgcttcta
     5281 aaaatccgtc cccataggtc ccttcggtga cccagtctgc cgccgtgcgt gtcagggccg
     5341 gggcatttcg cactgcgacg ccgatgccgg caacttcaag catctcgatg tcgttttcgg
     5401 agtcgccgat agccatcatc tccgtggggg aaagccccat ctctcttgcg agttcccgca
     5461 tcgccgttcc cttgttcacg ccgggtgtct ggaggtggac cgcaaacccg gtgtcgagca
     5521 ccttgacggg gagctggcgg tcatggatga cccttctcgc ttcatcgaca tcgatgttcc
     5581 gggcgaaggc cacgtccgcg aaccggtact gtgcgctgta gagttcgagt tcgacgccgt
     5641 ttccggcaaa atagtccttt aagacctcaa acgcttccag gcaggccgtc tggtcgccgg
     5701 gaatatggag ggtgccggga aaccccctcc ggtagacacc gccgttctcg ccgatgatgg
     5761 tcccgtccgt cccgaccatc ttgcagagac cgtccatgaa gcagacggtg ttcccgcttg
     5821 caaggacgac ctcgatcccg gcgtcgacga gggtccggac ggcatcgacg gccgcggtgt
     5881 tgatccgccg ccgccggtcg gtgatggtgc cgtcgacatc cgttactagc gccttcagca
     5941 ctgcttgagc ccggcctgtt caaccatgaa tgccgcctcg ccctcgggga ggtt
//
