LOCUS       DAOR01000001           11892 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_61312, whole genome shotgun sequence.
ACCESSION   DAOR01000001 DAOR01000000
VERSION     DAOR01000001.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_61312"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    180..463
                     /estimated_length=284
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    596..827
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11219..11448
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3689 a         2039 c         2095 g         3323 t
ORIGIN      
        1 ttgcgttacc aacttatgaa taatatggac tcataatcat agtgaacagt tgttaatcat
       61 tagtatctgt acattatata attgcaaccg aattgaagaa gggacttact tccatgtgcg
      121 cattacttct atttccatta atatcataaa gtacctcata atcatctccc catcttttcn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcttat tttttcatca
      481 ccatatacgt ggattgcaag gacttttcta tttccatcca aatccattag gattggcgtc
      541 acaagcttct cttctctggt atttgtattt gtatctacta caaaattaaa tatatnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca tccaatttca
      841 tatagttcta tcacactttc gaatctattg tctttgtaac ttgatggaaa tcttttgagc
      901 tcttttgcta gctcgatagc taggaaactt cctagatgca atctataata cctagccata
      961 tgtgcaattt ctttcctgta ggaagaggaa ttttgttcat caaatacaaa ggaagtttct
     1021 gggtagtgac actcccctat cagcgcggca tttgatgccg ccatggcata tttattacta
     1081 agaaatgtga ctattcttag tagtgaattc aggagatcgt ttttttcttc aagttcaatt
     1141 atatcttctg ctactttcaa aggatttccc attgaatgac acaatgtatt tgttgagtta
     1201 gagatgaagg gtctgaacgc atcagttgat ttctttttta gagtattcaa tctaaggcac
     1261 acctcatcca cttcagactg cggtttatag gtgaagatgt tctcaatatt gatgctactc
     1321 aaacgcgtta ctgaagctat aatggaatca gatgatagtc ctgatttctc aagaatcgtc
     1381 tttctatacc tcaacagttg aggagtgtcc aatgatgtta attcagataa cgacaattaa
     1441 gcaatataag tatagcactg tgtaaacaca ggttagaata ccatgtgaaa gattatccac
     1501 caatccattc tcgatatggt tcgagcccta tcggtagcgc ttacctttag tagactgcat
     1561 agatgacatt caaagattta aagaagacag caggcataag acagaacgct cagtaccata
     1621 gaaaaagcgg ccatacacta gcagcagtag gtgctagaca aaatcgcacc tgacagttaa
     1681 catatactcc atgtcaagaa aacatacatt cggaagttac ggaaatgata cccaaatctg
     1741 ctcctctgag ttcttttatg ctgtcgcgtt gcccctgcta gtactgtttg tataaactgt
     1801 aatatcaaaa ctatcaacat cagctttggt gagcgttcct ttcattacct ttagaaggct
     1861 aatcaatttg tctaaggagc tagtcgtgct ttttttactg caaagactga agtggtggta
     1921 gagctaggca ttaacaacac aaaaatatct ttcataatac gcatacttat ggattcaaaa
     1981 atcttacctc aagttaatcc tttgactgta tccataaata actggattgc cacatagtca
     2041 aaaggatctt gaaatgcaag gaagaattgc tgtattccaa catccacgta ttcgctaact
     2101 cttcgtgcta tatcttcagg ttttccaaca agggagtgtt tgagtaattt attaagcaca
     2161 gcagggcctc tctccattgc aaatagtctt ttcttatatt ctaagtctga atgagaattt
     2221 gcaattataa catctaattc aattgatttc ataattttgc ttgcatcgtt gtttttcaac
     2281 tctttaccgc atatatcttc aaatttcgac ttaacagaag caaactgttg aggagaaaga
     2341 taagaagatt cccatatatc ggcatatctg gctgctactt ccattgttct tctcccccta
     2401 gctgctactg tgattggtat actacttttg attggattat gcaggctagc tccattcatt
     2461 gtaaaatact ttccgctaaa gttaacgttt ggtttactcc aaagcaattt gagaactttt
     2521 ataccttcat caagtaatga aacccgatcc gctctattag gataatctat tccatatgga
     2581 taccaccact gtactgcgta atcatgtgtt gctccagcac cagtcctaaa ttccaatctt
     2641 ccttttgaca tatcttgtaa tgtcgtagcc tgcttagcaa ctagtgcaat gtttctatat
     2701 tgaggaatta ggtaggtgat aacaggccct agttttatcc gagtagttat ggctgataaa
     2761 ttagatatta cactccagca atctagatca atctcttcta atgattctcc gtaatagaat
     2821 ccataatatc ctaatttctc tgccaatatg caagtctctt tgatcttctc gtaatccgtg
     2881 ccccatattt ctaagcaaaa tcgcacaata gcttatcata gatattaact atgtcatata
     2941 tcaacgcatc atactgtaaa taaaatcgcc atgtggtttt gttacactta gcaaagagtc
     3001 tgaattaata gacaattagg ctaaggacaa tagtgagggg agtgcatgca ttctttggca
     3061 tgacttacta ctaggggggg atatcagtca ataaaaaatt ctaattatac acacacttat
     3121 catatcagta cctatttgtt cattccaaac tttcttttag tagccccaaa acatcatatt
     3181 ttttctccca taatctaaca tagtaacggt tatttcttag taagcattga ttaacagtat
     3241 gacgaatccc aaaaaaaggg attcgaaccg aggctccttc attatcgttt gaaatacata
     3301 ccactgaaat gatgtgggaa gacgatattc tactaagagg aggttcctac ttctatgatt
     3361 tcttaaatag taaagggaaa ttgactgaag ttggcccgta tatattagat tctaagatct
     3421 atctctcaaa tatagaagtt atttatcctt tgggtatatt caaatatatg gaattcaaga
     3481 tcccagaatc attagggtta gagcatgatg aacttcatag acagctcgtt aaggctatag
     3541 aagaaggtgg gaaagtaggc gaagccgcaa aggctgttgc agacgttcta catccacatt
     3601 tcgaaaagga agaagaatat gcgttgccac ctttaggttt attatcttct ttatcaaaac
     3661 aagataaaat cggttcagag atgaagagtg ttcttactat gaccgatagg ctgaaagcag
     3721 atcttccaca tatgattgaa gaacataaca gaattgtcac tgccctagat aatctgattg
     3781 aggtagcaaa gaaagaaaat aaaacagagt ttgtgcaatt tgcagaaaaa ctaaagcttc
     3841 atgctaagat cgaagaacag gtcttatatc ctactgccat attgattggg gaatatttga
     3901 agttaagatt atgactaaat ggtaatggtg aagattacaa acctaattaa atatactatc
     3961 ctggtgaagt atattttctc acgttggaaa gtacactgcg tcaaacggag ctctagccat
     4021 ataacaacgg tagatcaagt atgcaattca atgaagagtc atcttggatg tctataaggt
     4081 aaggtcattt gtgtctatta atattcaagg taaacttagt cagtgcattt cattttcaca
     4141 gcacaaacaa acacaaacac agtggacatt tgcatgtatt ttgattgaag agaatttgat
     4201 ataattgcaa tacggttgat aacaatagag gaacatgctc aaaattctag tttagaatga
     4261 tactacccat gggcacttaa ggctgctagt gaaaagccca gccgacgtta tccatgcttt
     4321 gaatatttcc aaatttgaaa aattatcgtg gattctgtag atgtaacata tttgcctttt
     4381 caattaagaa agaagatggc cgatgtgaaa tagcagtcgc aacaatgtag taagtgccga
     4441 tgaaaccaac tattagcata atgatgtctt attgcggatc acgttcttca tgatgtctac
     4501 ttccaaaata gtcttaatta aaacatgtac ggcatacggc tttaggtcat acctacaata
     4561 aatctatatt agcaatagta ataggtaaga taatcgggag atcggtgata aacgcttgaa
     4621 atatcaaatt atctgttttt attatgttta ttattgccct cacaatttta tcagatacaa
     4681 tgtagataca cattaaaggt aaaaggaagg aagaggtttt gtaggagtaa cggataatgg
     4741 caatctcatt agctgatgac ttaaggcgta ttgccaaagg tgagattctt tctgatagct
     4801 ggagtaggga catttattca gtagatgcta gtcattacac tataaaaccc tccatgatag
     4861 cctgtccatt aaatgaatct gatgttgaaa aaatctgtca ctattgcttt tcaaagagta
     4921 tacctataac agcaagagga gcaggaacgg gtcttcttgg acaatccctc tcaaacggta
     4981 taatcattga tttcacacgt cacatgaata aaattcttga ggctggtact gactatgtag
     5041 ttgtccaacc aggattggtt aaaggcgttt tagataaaga attgaaaaag gaaagaaaat
     5101 tccttcctcc tgatcctgca agtagtaact actgtacggt tggaggaatg gtagccaata
     5161 attcaagtgg tgctcattgt ttgggatatg gtaatacaat agatttctta caagaggtca
     5221 gagttgttta ttctgatgga aattctaggc ttgtcaatag tagtaggacc agtgcagaaa
     5281 aagaggatca taggatcaca gatttgttga gattattgtc agatcgtaga gaccttatct
     5341 cgaagggata tcctagagta acgaaaaact cttgcggtta tcgccttgat aaagtaataa
     5401 atgacaaagc cttcttgcct cataaaatat ttgctgcttc tgaaggaact ctaggcatcc
     5461 tgacttctgc aagattgaag atcattgata tcccactcta ccggtatttg attgtcgtag
     5521 gttttgaaga tttattatca gctatatcag ttgttccact tattttgaaa tactcacctg
     5581 ttgctttaga aatgcttgat agcacagtcg ttcgacatag agaagagaat agtaatatta
     5641 acctgcaatt tcagaacgaa gaaacaggtt gcctattatt tatagaattt gcaggcgaca
     5701 agcttgcagg agttgaacaa ggatttgata gttgcagtag taaattgtct gataaatgca
     5761 ccataataga gagtgctgct gatgaactga gcttgattcg aatgtgggga gcaagaaaaa
     5821 atgcactgaa ccaagctatg aaattgacag tcgggagtag aaaacctatt ggattaatag
     5881 aagacacggt agtacctccg gatatattat acgatcatac acaacaactt ttacaaatgt
     5941 attctgagaa taaactagac tatgttatat atgggcatgc aggggatgga aatttgcata
     6001 caagaccact aattgaggta gggtctcaag ctgaaactca actaatggac catcttgcca
     6061 ttcaactctt tcaaagagtc ataaaagctg gcggaacgat tacaggtgaa catggcgacg
     6121 gattagctag agtaaaatac atagaatctg tgtatgggcc agagatctac tctcttttcg
     6181 gagaaattaa gaaattattt gatccaagat caattatgaa tgttggcaaa aaggtaattc
     6241 aggttaggaa ttaataatga cttaccacca agcattaaag cacttgatca tcgttattac
     6301 tgctatcaca caaaataaaa aattgaaaat tcctgtacac tatatagttt taacagtgct
     6361 taaacaacat ttttgatcag atcttctgtt gcaataattt caaacgaatt gcatatactc
     6421 aaggtgcttg cttgactagg atctaatttg aaaaaggagg tttgtatgca aacgtcttcc
     6481 tcattgcaac aagaacatca aaaacaagac attcctcatc ttaaaggtat ccttgatgac
     6541 aattcactta ctttgcgtac cttgcttgtg gcgatctcac tatttttctc aagactggtt
     6601 ttttcatgag ctcgctcctt ctttctcata cgatttaaga taaggtatag atagtaatcg
     6661 ataatgatag agcagttaat tagacatgtt ggaactgttg gtaccatacg ttcttaaatt
     6721 tcctagaacc tacagaactt atgaactcaa tcaaggagcg tataggagtt gttgcgggcg
     6781 gcggtcactc aatgaacgcg gcagcagcaa taacagcaat tgccgcagct gaagcggccg
     6841 gggtaaggca aatctggatg aaccaagaat atctagatac attgacgata tttgcagctg
     6901 ccaccacaaa aacatctata gtgcgctttg gtacggcagt tgtacagacc tatccgcgcc
     6961 atccgttagc tttggcacag caggttttgg ctttgaatga tatcgcacca ggtcggttgc
     7021 ggcttggaat aggacctagt catcgttggt gatagagggc atctttgggc tgccccagag
     7081 atcaccctat tgcaattcag cttttactct ttttaccaaa ccatccatat cgactggcat
     7141 tctattgaat tgccctccac tattttccag ccacctgtga gagcctatcc acttttaacg
     7201 ttgactgcga tgtcacaatt tttatgtcta tcatattagc actctatgtt ctaaatactt
     7261 ggaaactcat agataaaaga cgaccaagct acttgcatat ttgtctttta ttataacatg
     7321 tcatcaacaa ccgaattaac agacactgca tatgatattt taaaagtatt gggtaaagat
     7381 gctgactttc tgtatgacac aatagaaact tacatcaaag atgcacagaa atccaataaa
     7441 tcagagttgg tagaaatatg gcagacaatc aagaatgata gaaaaagaca tatgcatatg
     7501 cttaaagacg cattagaaaa agagattcac agatagatct tgaattaagc tgagctgaaa
     7561 ttgattctaa ataagaacac gattaagcgg aatcatcgtt tcatcaacat gcaagcctca
     7621 catactcatt gattgcatgt aattacaaaa ttaatcttgt tctattttac tttcaaggaa
     7681 ataatttgaa cgaggtaaag aaagaccacc atctgcatat atcatacatc ctgtaatata
     7741 gctcgaatca tccgaggata gaaatagcgc gatcttagca atttcctctg cttgtccaat
     7801 tctttggaaa attttttaat atctactcaa tactctcaaa gagtatgctg tttcttggtg
     7861 cggtgattct tctaaaggga tgaatgctgt aagacacttt gtaaatacga cacattccct
     7921 tattgttgcg atgcctgaca aaccatggaa atgttggtat gttgataaga tctatggata
     7981 gaaatgaaat caagtaatat gcaaaattat accggcgtaa gtcaaaatgt atatgtactt
     8041 tagcacagag atcttacagt atcatcccat cgatcgctgg cttagtataa gagactttat
     8101 caatgcaaat atttttaaaa gcaataagac atttccggca gtatggggtt ggggacggtt
     8161 ggttggtcga ttctggtcat ggggatttcg cttgctacgg tcataatttt atgggtatgg
     8221 attggacata taggaccaac ctactcttca aacactttaa tacaccaaca gaaaattcta
     8281 aggcagcatt ttggcctacc acctcagcct gtcattaccg atcctaagct actcctcact
     8341 cccccttcgc ttagagacgt taagaataat actgcgaata tgaccacggc ttctggcgga
     8401 actaactcaa ccggcggtat tagtgtttca atcgtaccag gagccgctac cttagctgac
     8461 aaagcatttt caccaaaccc tatcaatgtc aaagtcggag gtagtgtgac atggtctgac
     8521 aaagacacca cagccccaca tactgttaca tcagggacag gatctagtga tccaaataag
     8581 gggaaagagt ttgattctgg attatcaacc ctgctaacag caggaaaaac attctctcat
     8641 accttcaaga ctgcaggaga gattccatac ttctgtatta tacatcccac aatggtcgga
     8701 aaggtagttg tgaagtaaac tccctccatt ttttcaggta tcaactaatc taagatcttt
     8761 aaagccgtaa aaacgtccat acaacctgaa gcagttggaa catcacattg acagcgttca
     8821 tgaatcataa tattacttga attgcactca ggacaatgcc actctcattg atcgtctact
     8881 tcaacatcta tcgtagagaa tgaaaactcg caatgtcgat tgtgacagac aaggcattta
     8941 tcatcagaag atccttcaaa atactcatgt ggtgctgatt gccctcggtc gaatactgaa
     9001 aattaactat ctcttaggtc gtatctaaaa ccgacgatct tactggtgtt cagagactga
     9061 aaatactatt caggcagacg tctcacatag atttctacaa gccataggga attgtgggga
     9121 agaaaagcag tatcctgtta aaaagaattg gagatactta ctgggagggg agagtcatga
     9181 ataagaatgg ccaaaagaaa tactcttcta ccttctcttc gatcacgatg cacatgaaaa
     9241 tatttcaacc tcattttttt attaggaaac gtaaacatca ggacttaaaa aaagagatga
     9301 ggttgtgtta ttttcttttc tcacaatcct ttactaattc atttaagaaa tgagactaac
     9361 cttcatcatg ttctttgaac attttatctg ctttaaaacc agtatgcgat agtccgtatt
     9421 tgaaaagtag gcatgcagtg cgttgcaatg caagcatagg tttgatataa cactctgcat
     9481 gtaactagaa gaaggcggac gtgttaaaat atgaagagtt aaaagcaaaa gatttagcta
     9541 gggaagccat tgcactagaa ccaagcaaaa ctctttatga tactcgtaac attttactga
     9601 gatataatat tagcagagtc gtaatcgcca aagataacaa gcctttagga ataattacag
     9661 agaaggatat tgcacggttc ttgtacaaag aggtgccaaa taaacgtctt aacgagatta
     9721 cattaaatga aataatgaac aaagatctca taacagttga agaacaaacc aatctgaata
     9781 tctgtgaaag gatgatgtta gagaataaga taagctccat aatagttgtc gataatcgag
     9841 atctaaaggg aatattcaca aaaagtgatt tggtagacgc atatgcaaga cattatccaa
     9901 gaaagctaaa ggtagagcaa tacatgacca aaaaagtcat taccgtagcg cctgatgaaa
     9961 atctccatat gattctttta cttatgagtg ataatcaaat atctagagtt acagtaacaa
    10021 gagatgataa actcattgga attattacag gacatgatgt actacctgta agtatacttt
    10081 ttggaactgg aacacctagt atgttttgga taacccaaga ggaattgata gcacgcagac
    10141 gagcacacag gtttatacca gctgggataa atgacgtctt tttagcaaag gatataatga
    10201 aatacgaccc tgtaactatt acaaaaggtt ctgatctagt agaagctgcg caaataatga
    10261 gaaacaatag gataagtggc cttccagtag tagaaaacga ttcaattgat ggcggtttgg
    10321 tgggcataat cactaagaca gatatagtta aagcaatgag gcacaaaccc aacaacgcaa
    10381 aggctggcga acgaattgag atagtttatt aactctatct ataaccgata agccaagatt
    10441 ttaaattccg ctggcgcttt ctcccaactt gctttcaaca tagtgtcaag tagtacccta
    10501 ccccccacga caacagagct attacatatt actattttgt tcaagcgctt tgatcgttac
    10561 caggacaatc tgttgccttc gacgatattt gcgttagtcc agacaagtga ctttgtaaac
    10621 gaaaaaatag gtcaatattg gaatgagaaa gtagaagaga tgacaactca atgactataa
    10681 cagataaaga ccatttcgta ttgttaaaaa cgattagtgg aataaaagag caggtcggta
    10741 aaatatcaat atatggaaac cacgacatga aggatatgcg taacgagttc gacggaatga
    10801 ttgaaagatt gattgacgta gttaatgagc tacataaaaa ggaggaattg acggaggtta
    10861 aaacgaatgg ccagtaaagg tatcattgtc agtatagctg tttacagcta gtggcgcatt
    10921 tccttttttc caactacgac tgaggccgcg atcgcaaatt caaaatcaaa ctcaaaacca
    10981 aacgcaattg agataactta gctgctaaca gccgaaatag gaattactga taaccagaca
    11041 caactaaaca caatctatgc gagaggttgt attccaaaat tatttttggt aacagtcact
    11101 atgaaggtgg taagaaatac tgtagaatga gtgaagtcta ttatcaccac gatggtgtgt
    11161 tctgtacttg ttgtggtatg gcattgaaaa cgttaccaac tagtaaaaga gataaggann
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta taaagggaat
    11461 aaagaagtag cctgtcgaag aaggcagttc aaaatgaaat gaattcgatt ggtttaacag
    11521 tctagatgaa aatttggggg ttgattcaga ctagaatgaa ccccctctta caataagaaa
    11581 cctacgtgtg aagcatacta acacaactgt tccaataatt gagtcagtgt ttgaatttgt
    11641 cgatataata atatattagt cctctggtcg aatatcctac gatatttact aatttatggt
    11701 actccacaga ttttcttgga tcgattagtt tgctgccctt tacatgtgcg atggaacatc
    11761 tctcatttgc ataacaattc ttccagcttt gctcaaatcc aacaactacg gctagaaaag
    11821 acaaggaaga aataaagcaa aatcaacact aaccacttaa gttataaacc atctcccaga
    11881 aaagatattc at
//
LOCUS       DAOR01000002            3113 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_52209, whole genome shotgun sequence.
ACCESSION   DAOR01000002 DAOR01000000
VERSION     DAOR01000002.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_52209"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1014 a          550 c          601 g          948 t
ORIGIN      
        1 cttcaaggct catgaaacag ataagacatg aaggtcttaa accgatgaga aggcagtatg
       61 ttcatttgtc tgttgacgag cagacagctt tgcaggtagc caaacgcaaa gagggaacgt
      121 ctgtcatcgt tacgatttct gcaactaagg cgtatctaga tggaataaag ttctatcaag
      181 gcaataagac cacgtggcta acagactaca taccatcaga atacctagat tctagtacca
      241 cgacaacact ttagtagtct ttggaactgc atcacaattc ttgacaattg gatatccttg
      301 ttttggttca tgcatttctc cggggttgct ctgctcatac cgataaatgt gcccttcgtc
      361 atatgtgaat aaaatttctc gtaaaaagta gagtccatct ctgctgtgtt aagaattgag
      421 agtaatagcg aagccttttg gcgtccttgt caggatagaa tactcctgtc cacccgcctt
      481 tgtctgaagt atcagggtaa ttccaacctg aacagcctag cagaaattct cccatctatc
      541 aatcaatttt cctatccagt ctatatattc tgatattaaa tatcttagat tattatttta
      601 tggcgctatt aatcaatggc ggtccttgcg tttttgttaa ctatcataaa tttgtatact
      661 tttacgagtg tactataaaa ttggttacac tcctctgtat tctagatgtt tgatacattt
      721 agaatcaaaa ctgatgcctt tatttgggcc gatctagtct ttccatctca aacaacgatt
      781 accacccaca gcaccctaca gcattgtttg gaaccctgtc agtcctagga agttgttagt
      841 gcagttgccc acgctggctg aattgcaaga agaacgtcaa cagtagtcat tgggaaatgg
      901 aggaagagtc gtaaagaaat gagagcctgc gaaacttgta gtggcttatg aacattgtta
      961 gaattatccc tctccgaatg attgaaccta tgcgtaaggc attttcatgt gcaatgccgc
     1021 ttgtgcaaca aatggaggtt ttatttacat ttctaactat agtcttggag atgagtgatg
     1081 atttcagatg taaagataaa ttcgttatgg ataatcaaaa atttttggag atgacggaga
     1141 acggcatcct gttaacagaa tacgacatga cgccacagga tgtgactagg gaagtcaacg
     1201 taggtaaaga ggttataatg ttaggtggtt ggaaatttaa cgtctcaaga gtgatttaca
     1261 catacgctac gataaatgaa attgaagagg gttggaaaga tttacttcat gaaaagatac
     1321 ctgtcgatat tcttttttta tctctcttta ttagaaccat tcttgacaga gataaacctg
     1381 aatcgatagg taaggagccg gaattggttg tccttttaaa actatgttac tggtatcata
     1441 aatgtctaaa aatggatagt aacaataact ttcagtataa gataaatgaa ataagggatg
     1501 agctaggtga agggttactc gctaaggcag agcgatatca attacctgta cgtgtaaatc
     1561 ttggcagtga cactcggaaa tatttcgaaa acttacaacc aatattattt gcaatcggaa
     1621 aaaggaggtc tcctcctggc ttcactgata tgttgttgga tattcgtcat tcagtttata
     1681 gattcgtgtc tgaatatctg ttttactcat taggtgatga ggctaatttt atgatatcca
     1741 taattaaaaa taatcgacac caaaaaactc ccgacttgta cctcaataca attccttcgg
     1801 acgtgaaaac tatcgttgat aaaatggaat atcaggaaaa aatagaggat aatctacttg
     1861 aagaaattct ttgttctctt aagcgaaata agatatttaa aaaagtaaac gaaggtctat
     1921 cacaaggagg caaaattata attcttgacg ctactgcacc gtctctcgga tatgctatta
     1981 attactatgc atcaactcat aagaaaactc ttactataca aatagcgata aacgattcaa
     2041 ttgtgtttgc aaattctaac tctaagggat atattcccat tttaatattt gctcaatcta
     2101 ttgatcatga atgcaatttc aggttatctg cagtcatggt tccatgtcct gtaatgaatg
     2161 gtaataaagg acttgaggta gacctttcga aattcccgtg atgaaattca aatagaatat
     2221 agaagattca gtgtttctat tgatgaatag agattggcat ggcttgtggc cttttcattt
     2281 ctaatcacta taatgaaggg attttgataa aaagaaaaaa ctctcctttg agtagtcata
     2341 tagacctcaa taaatatttt ggaaaataga gttggagcat cgaccaaatt atcaagcgta
     2401 tccaggaatt agccaaggta gcatttgaca ggtatgacga tatttcggcc ccatcaatgt
     2461 gactccaact aggctaacat taatagctgg gccgggcgca tccgtaggta gtgttagagg
     2521 ctccgcacca cggcgaagtc tgttcataga ccattgagcc aatgacttta ggacattttt
     2581 gtcatgtcag tcgaatcaac ttttaaagga acatttctgt tcaagcataa ataactatca
     2641 tcgaaataac attcaaaaaa ccggtctaga cccagaggaa aaaagaaatc caagttataa
     2701 gttcttttta ctttctgttt tgcgatgcta tatgattacc agcagaatca aacgttctac
     2761 taaggttcca agatcgataa attacgccta gttaagccta tttatacaga ttagaaacgc
     2821 ctataaaatt gttagacata ttccttaatt atcacaatcc cctaacagtt tatcaaatgg
     2881 ctgattgccc cctatgcaca ggtgcgagag gtatcaataa aaaagaagaa tttattgagc
     2941 atttagatag ccaccaagaa atggcgatga accatgacat gatgttcaga aagttcaagg
     3001 aatacgtatt aactcatgaa aactttacgg aatgagacta aaatacgggg gtatcatagt
     3061 ttaaatgtac tatttggtgg tagtagacat gaccttaaat gtttaatcaa tta
//
LOCUS       DAOR01000003           13081 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_37093, whole genome shotgun sequence.
ACCESSION   DAOR01000003 DAOR01000000
VERSION     DAOR01000003.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13081)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_37093"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    199..481
                     /estimated_length=283
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3950 a         2421 c         2301 g         4126 t
ORIGIN      
        1 aatttcattc aatccaccag ttcgcatgaa ctcatattta ataatctaca ccttaaatgc
       61 attgtataaa tttcctatga ggttaaatag aaacacaggc cctcctgtga tagataaagc
      121 tactactgaa gaacttttta tgctaatgcc acagaagcaa ctggtcaagg gttccaatca
      181 attagtgaaa gtttctgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 ngtttctgaa atgtaagaag ggtttcaatt aaacttatag aaaccgatct cgaaaagaaa
      541 aataactgac gatcaaccat tctattgtgt attacataac cattctaaaa acattctgaa
      601 aacattctgg aatccattaa tggtgcgatg catactggcg ggatgtgtta atgacatcta
      661 tcccctcagg gtaggtaaat ttttgaaaaa ccgattgtta ttgttaccga ctgttttttg
      721 aaaaagtgca atgatcgaag ttattcctat acggagatgc ctatgtgaag gttttgaatt
      781 aacggcttgt gactacgaag actttcctga gtggaaaact gagtgccact ttgataatac
      841 ctattgaaat ggcacgtcga cgcggccttg aggaaccctc ccacatagtg gttgaagaaa
      901 gaagtgatgg aatactaatt cgaaggttcg agtgaaacgt cttgggtgaa aatgacggct
      961 aaaagtggct tgaataacat agtatattcg cttgtcgatt ttgacattct tgtaaagtat
     1021 agtatgacgg tactgtaact aattaaatgg taatgatccc tactttacag tttaccgtct
     1081 acgcacaacc tactattgtc ttgttaagtt ttgttgtgct gtctgctgct gtgttggttg
     1141 tagtggctgc tgctgaaacg gcgtttttgg tagttggaaa aacggaaatc caccaaaact
     1201 gctactacca agagcataag gcatttgacc attccaccta ttgacggcca gccactgaag
     1261 atactgtgga ctttgtttta attgtgaagt aatgacctgg attgcctgag atttgccgtt
     1321 ggccttggct acatttgctc ttgctgttgc ttgggcttgg acaattgttt ggtctgcaat
     1381 tacctgtatt gccattagac tggtacataa taataatcct tgatagaagt ttccggtatt
     1441 acagcgacct gttgagagtc tcccaagtat ttgagaacgt tgaaggtatt gcggttcata
     1501 tcttaaaaga cttgctatac tggttaagca ccctgttctt atgaggattg ttcataaggg
     1561 aacaccctag caaaagacac agaaatagag ggtagcacac ttcgcagtgc cttcatccct
     1621 tcagccgagt cagcgtagta tttggcgagt aagttgcaag tataaaaggc tatttaggta
     1681 actaggtgat gttaagtcca acctcggatt aaatgacgct atggtacagt tatgcacaaa
     1741 actattcatt atctattatt tcccctacgt tacaaaatta ttttcttatc tattagaaca
     1801 cttcaacttc atgcagactc acaacaccat tatcattgac cattctcttg atctgaggaa
     1861 gcaaaggttc aagtcgagat tgttcatcaa ttacttccat caccagcggc atattgatgg
     1921 ttattccttc gaggtggatt gtagatctcc tccgttttcc aaatccatca acaccagtcc
     1981 agactgtagc accagaaatc ttagcttcca tcaaaaattc tatcaatacc ttgtgcaatg
     2041 gtttgccatg aaattgatca tttctcttta tccttatcgt gagacaccac attttcagtc
     2101 tcatatcata aactacctct tagcactata agactggcca aagctcttcc tccaatcaaa
     2161 aaacctaatg ataatcctac attagccatt atattgattg cagctgtata gaatagtctg
     2221 ttatcaatta gattgcttgt ttcaagagca aatgaagaca tcgtagtaag agagccacag
     2281 aatcctaccg caacaagtat tgaatatctt gtatctagat tccaaaaaac ggagacgatt
     2341 gaaaatacac ctaaaatgaa acttccaaca atgttgacca tcaagatatt tactggtagc
     2401 acccctccta aaatcagcgg cgaagaaact atcctgtatc taagaaatgc gccggccacg
     2461 gccccgatta caagaagaat gaattcgatt cctttcattt tttaatcgta gcttacccct
     2521 attccatttt ttattattgt atttgggtag gcattattag catcgatttg acaactaatg
     2581 cggttttggc atgctgcgca tgagaagcta gccttggcta atgccatagc cagaattcca
     2641 tataaccaag caccttttaa acttattgca aaacttacta tttctatgag atacactcta
     2701 tgctcccatc taactgagga agactgcaaa ttacattcca tatcattaac tggctaaaca
     2761 ttactcaatc cgcctcataa gtgtaataaa agcaaataaa aagaagaatc gaggctcatc
     2821 tcctagttga tttcaaaaac aataacctat atgtttgttg tagtcatcgg aattgcaatt
     2881 atttatctag ggattagatt cacatttggc acgtataatc ccttctatgt cgttgctagt
     2941 ggaagtatga tacccactct gaacatcagt gattttgtaa ttataaatca aaacatacct
     3001 tttatgaatt tgaaggttgg agatatcata gttttcaagt cgcctggctc attaatttcc
     3061 aacgagcaac acgagactat agttcacaga gttgctcata tttcgactga catagaagga
     3121 gacagaatta ttagaacaaa aggagatgcg aacgatggtt caataccata tatcgactac
     3181 cctattagag accaaaatta catcggtaaa gtggtgtatg atatccctaa actgggttta
     3241 gttactaagg tgattagtcc gccaacgaac tatattatca ttggagttct gataatagtg
     3301 ctggtctact actcgagatt cggaagaagt gaagaacaaa aacgggtaac ataaaaacga
     3361 gatcgcctcc taccttcaat tcaaactttc gaacggataa taaaagatca taccaaagcc
     3421 tggcggggac ggagaaaaag gtggcactac gctagtgaaa attaagaatt ctactgcaac
     3481 ggcacaaaca aaataaaatg tatctacgca ggtttgatat tcggattttg aagagtcact
     3541 ttggtattta ttgcgttata gtagcagata tcttatgaaa aatattacag atttgtacat
     3601 aagataaatt aaaattgtct ttttatcaaa tatatgtaat gcaattttgg cacataatag
     3661 aatactacaa tatcacgatt gcaattgaaa aagtgatatg atatatgcac acattttttc
     3721 acataataga aagtctagtg cattcatgag agtcatgctg cttaggataa gatgtgactc
     3781 aaggaaatat tctataatca aaaagtttct ttaagttatc ttctgcatag atccagcaac
     3841 cacctaaatt aattgctgcg ttagcctcat aaaatccggt atctgtctct gcagggatcg
     3901 tggcatcgat gcttctgcag acgcagttac catgtatttt ctgttctcca gcacaataat
     3961 actacctcat gcataaaata cattctattg gacactattt ctattgttgg atatgtcctc
     4021 atgaaaaaaa ccaagtatca aaaaaaatat tgaatgtctg aatctcatat tggagaagat
     4081 atttaattcc gtagcggggc ataaaatgat attatataac ttgcattatt attttccatt
     4141 attatttcat atctaatcct aaaatcccgg catttcccca attttctttt gaaacttcat
     4201 agattaaaat tcttaccttt tcctcttgag ttttcataat ccttgctgtc tctttcgtca
     4261 atactttaac tagttctctt ttctgttcaa cagaccttcc ttggcttatc gtaatttgta
     4321 ttactggcat atttgttaac tgtgctcaca tattatttat gatttctata cattcaagtt
     4381 ttagttatca ttcggattat gcttgcatat tgtgggcgtc gggttgtact attcttgcta
     4441 tccactacga tttggttact atacaagtct tggtctgacc atcagtacta gataaaatat
     4501 ctccatttga ctttctcgtc agcagttcac acgatcctgt tgtggatgag atggagtaac
     4561 aggaatccct gaacctgaac ctacaagatt ccattgattt gctacaaata caccgctaat
     4621 atcacattta gaaatacctc cagattcatg attgattacc ggagacataa gatcgccatt
     4681 gaaattagca tgacctagtc ctagagtatg tcctatttca tgcataacta tttgctttaa
     4741 ttgggctgtg gtgaaagtat taccaaaggc acttgttgct atattcacaa aggcacgatc
     4801 tacgtagcca ctgccatcaa agtaagttgt tgtctgtcct actgtatctc caaaactgga
     4861 tttgccacca atagtactgc tgccagaagc agcgctattt tggaatccga atattatgtc
     4921 tgcagtactc ttttttccag aaacctgtgt cagtttcaat ccttttacac tcttcatcca
     4981 tgtatcggca gcagcattaa gagcttgacg ttcagctgta ttaccaccga ttgtcttata
     5041 tgttaattct ccatttgtaa attgggaatt ccaggaacaa caaactagta ttgaatgagc
     5101 tggtgggtga aagtgtttaa taatactatt gttgtttgca tgaccgctgg tgggaggagt
     5161 atgattatta tgatgatgcg atgaggcttc tgcgtatctg ataggcaaga aaatataatc
     5221 atttggttgc cggggcagta tagaagcggc tgacagaacc aataaaaaag atgccattat
     5281 accatagaaa aatgccggct tcactaaagg gatattttca ttgctatata atatggattt
     5341 tttaaaatat tactgtattc ctactaagga gttaagctat actaaaagac tcttctattg
     5401 gttacccata tatcatccct accaatcatg cataaatgta aattataaaa atcttctagg
     5461 tcccagagtc tcagtacctc cattctccga ttatattatt ccaccaagca cgatacggcc
     5521 tagcatcctt atctatctcc tctttgattc taaattcaat tgaatagaac atattctcca
     5581 tagcatcgag tcctccatct gcgtacaact cactaccgat ctcttctgct cttttctttt
     5641 ttgctccaaa atctggatca tgtggatttt gtaggcaata gatgagaagg tcgtaaagtc
     5701 ttcttctaaa tatgcatcca tttcttatta cgtatcatat gaggcaaaaa gtgcctatct
     5761 atacgtaacc attctccgtt cagatattat attagtagta tatgacgaga aactgcacac
     5821 tctcaagatg aagtagtagc ttgacttcct ccgtatttct taacatattc acaatccatt
     5881 tcggattgtg cttgtatatt gcgggcatcg ggttgtatta ttcttgctat ccacttatcg
     5941 acttggttac tatacaagtc ttggtctgac cattactaga taatatatct cgacttgtag
     6001 attcctgata atgattctca aaagttttct ttaaatgctc atcattttct ggtagactaa
     6061 gagataggtc aactacggcc gtatctttac gcaattcaag atatggttta gggaatcttt
     6121 cctcattgaa gcttcctata cgtcttgtgt ccacatttga agatgtagac atttatccat
     6181 ttcaacatct cttcaatcgt tccactaagg catctactcc ttaaggaagt caataacaaa
     6241 tttattgaaa agcgttgagc tattcatatg tactgaatga ccacaatttt caaggagaat
     6301 tactctacag tttttcattt ttatgaaagg atcaagaaac tttacaggaa tcatttgatc
     6361 atcttttccc caaattaata gtgttttggc cttgattttg tgtaatcggt catttaacct
     6421 aggtgcatgt gcactcccac taaaagcgga cataaatgcc tctttagctc taggcaattt
     6481 caacctctga aaaaacattt ttgcgtaatt atattcaata gatttgacat ttttatctat
     6541 gcctaagaga tctcttctta cttgttgcac tgtctttgcc ttcacaatat tgatataatt
     6601 atgtaattca ggtgttccct taaatgaaac aggcaaagcg ccagcagggc ttactagtac
     6661 aagttcagag actgctaacg gataatttat agcaaactct gctgcaatat ggcctccaag
     6721 ggaattacca atgatacaca aagaacgaga atctaacctc aatcgcctga taaattttga
     6781 tataaacttt gagtaatatt ctatattgta cgttattttg ggcttatcac ttaggccgaa
     6841 tccaggtagg tcgagtgcaa ttacttgaag attgtgagat gaaattgcct caacgttatt
     6901 tagccaactt tctatcgagc cacctaatcc atgaatcagc aagacaggat cacctttgga
     6961 ttcagtggca acgtaacgga cacgaacatt agctactttc atgtattgaa aatgcatatt
     7021 tagcatctga gctcttgaag ttccatccag attaataaat aatgttataa caataaaaac
     7081 tgttcttttc ttgtatctgc caccaaagta ttggtaatat gtacaaagta gtacgaacat
     7141 taccaaagcc ctggatgcgt ggtgctaatg tttgtaaatt tactttttca tggctttgtt
     7201 ttagcatgca agaaagtaaa ggacgataaa aataaaccta tttaggttaa acgatttcgt
     7261 ctaaagcatc ctgctgcaga aaaactattc taaaactggt ggactgtgta tcaatagatc
     7321 cggtcagcaa ttattctggc attacaattg aagttctact tcgttgttat tttctccgtt
     7381 atatcgccta aaacattcct atatactgac acagctatat atccacatac aagtaccatg
     7441 aaagctcttc cacacgttat gagaaatatc aatgagttta atataccgcc tggcgattct
     7501 aatggttatg agatactgta ttttggtgta aatcttatac ttatgtttga ctataggctg
     7561 tcttttgata tagaggtaaa gaatacatat agcgaaattg gagttttgat aattagtaga
     7621 agatacgttc cagcttggaa aagtaatgcc actaaccagg atctgaatac acattatggt
     7681 aatcatggga caagaatcaa tgatgtcttt aaaccatcag caagtgatgc atatgcattt
     7741 attttagaca ataggtttcc ttctgtagga gactctgaaa ccaaaaaagt agacgttact
     7801 cttgttcata cctggcaaca agaaataaga gaatcacaac taaaagacag ggaagttaat
     7861 gacttcaaaa gatagcaatg catctcacaa tactagacca ttaatttgca tatatgcgat
     7921 attatgaaaa tatcgagtta aaccaagatg atgatcaatg acaaaatttg gtgaacctta
     7981 tgctaggttc gttgctgcaa ataatcagac aggaaatgaa gaaacgagat tgattggaaa
     8041 gagaaattaa gttctattgc cctcgctgtc ttactaattt gataatacac atatcttttc
     8101 tttccaattg gcatagagat acgaaccatg tgaattttga caatctggat caccatattg
     8161 acattagatt gcgaaaagta cctttgcatt cttaaacatt ataagatctc tttcttggaa
     8221 gttgagtttg atcttttgga aaaaattgaa atacacgccc atgtcggaaa ttggccaatc
     8281 tgttccgtat aagaggtacc gtggttcacc tgcataattc aataactctt ttatcttttt
     8341 tcccaaatat ttttcagagg ccggtgtaaa atcgccatag aacaatcctg atatatctgc
     8401 gtaaacattt ttgttcttgt ataatacttc ttggcagtct ataatccatg gattccctag
     8461 atgacacata actatcttta gatcagggtt atcaacagca acatcatcga tatttattgg
     8521 atgtgaaaac ctgagtttac cctcctttag aaaagtatct cctgtatgaa tcatgactgg
     8581 ggcattaaat tcgacacaca aatcatacac tcgttggtat ctcttatcat aaggatagta
     8641 gtgttcgtag ccacagtaga gctttatcgc cttaatgaaa ccctttttga accatcttct
     8701 acaattctga aggtcttcgt cactatgatt atctattgaa aaaccagcaa ccacgccaag
     8761 gttatcgtgt tttttggtga tttcaattat tttggaggtg gatggcctat ctgaagtaac
     8821 tttataggat gtaagaataa gtgagtaatc aacatcataa ttatccatag tatcaagtaa
     8881 tgattgcaat ctctcttcta aaggaatctt agtagcacac ccgtattggt tgagatggac
     8941 atggcaatct atgatcatga aggttacact gtattatgtt attgagaata atatgttatt
     9001 aaatatcccc tttatctcat agcaataaac acagataagt taattctaag gataatagaa
     9061 tctgtgtaga cggtcatttt ggcttttaag tatctctttg ttggcttaat tatcgccgcg
     9121 tgattatatg cgatatatct tcttttaaca tggtcccttc ttggaccatc aactgtattg
     9181 tttttactat tattatttta ggacgctttt catcactaat tttctaaact ctgtgtcaga
     9241 actattttct cttatttcca taagcttaaa agcatcatca cctaccagat accttaattt
     9301 tggtgggttt tctgatgtaa ctgcttttaa gatgactttt gcgacttctt ttgcagaaga
     9361 accgttttca aatctaggct taaatcctga aaccctcttt tgtgttattt ctgcatacgg
     9421 tgaattagga ttactagata cgctttttcc tatttttaaa ttaatgtcaa aattagtttt
     9481 tgttaagcca ggttctataa gtatactctt tatcccaaat tgttctattt caaaagccat
     9541 agattccgat aatccttcca aagcaaactt tgaactaacg taagcacaag taagtggaaa
     9601 gcctatccta ccgtttatag agctgacatt cactattgtg ccatgtcctt gttttctcat
     9661 aataggtatg acatgttgta ctactctgat aacaccaaat acattagttt caaattgttc
     9721 tttaaattct tcaatcgaaa gctcttctaa tggtcctacc aatccataac ctgcgttatt
     9781 aaccaacaca tctattcttt cttgttcatt tacgatctta tgaattgctt ctttaacaga
     9841 tttatcgtca gttacatcaa gttgtataat atgtaatggt agtttgcttt tattggcact
     9901 atctgaaatg ttgccggatt tgtcaaggtc acgcattgta gcataggtat caaatccatt
     9961 ttttgctaac aacaaggagg tttcaaatcc tataccagac gaactaccag taactactgc
    10021 aaccttcttg ttcttattcc tatttttgta tcccaattgt atatcacaag cttaacagca
    10081 tcttaatacc tcagttctcc gcttatatta ttccaccgag aacttttctt tcacgcatgt
    10141 cataagcatg agattatatg ttaaaaacat caaatgataa gttgtatccc taactcagga
    10201 tgttcaatca taattgcggt ttgtctgtgt attttttttg tccaactcat tcagaacatt
    10261 tgtttcaaca tctaccgaca aattttgtat ttgctgcccc tagctatagt tgtaatcaga
    10321 tatgaaggcc caaaaggagg tcctggaatg cgagaaatgc tctctgtaac agctgctcag
    10381 ctgggtcaat gattaggaga aaaagtcgca ttggttacag acggaaggtt ctctgggccg
    10441 actaaaggat ttatggttgg ccatattaca cctgaagcaa tggtcggagg agcaatcgcg
    10501 ctagtaaaga atggtgataa aattatgatc gatacaataa aggggaagat cgatctgttg
    10561 gtatcaaagt cagagttaaa tagaagaaag agaaaatgga gaccaataaa accacattat
    10621 gcaaaaggga tacttgccaa atacgcctca ttagtgagct cagcctcgat aggagccata
    10681 actcttccgt tgatgtgatc taacacatag catgacataa tcaaatgaaa ggttaattca
    10741 attcctgaat gatggaagaa attgggtgag aaatgccacc agtatacgag gtatttttct
    10801 attcagacta cctgcttcca aaggaagacc ttagctattg agattaatcc aattgatgcg
    10861 tacggctcta ttacaaagaa gagaggagta gtaataaagc ctcgttcgga attggctgaa
    10921 attagcaagc cttagtcatc cgaaagcggc tgggctgacg aaaaggatag gcttagtaga
    10981 tccagaaaag tatgagtctt atgtgaatct ggatgcctta tttccatttc gagatccaga
    11041 aagagttctt ttaactgtag caatgaatac atgatgaaaa aaatgaactg atgatcagag
    11101 tagccgcctt gaaaatatac ttataaaact aggataatgt ctaacaatgg tatatcacca
    11161 tagatctgat gcgggggttg ccaagcccgg tcaacggcgt gagatcagac agatgtgtcg
    11221 acacgacgct cagatgttat agcctgactg aggtacctca tctcttaggg gttcgtgggt
    11281 tcaaatccca cctcccgcac cttcacatat tcttccttct tattactttg ctacttctgg
    11341 aatgagactc ttatggtggc atagtatact attatataga tgacagaaac ggtcttgtca
    11401 gcggctgaaa aaatactaac gagaactgtc gtttcctgta tggttaatta aaagcttctg
    11461 ctaatttgaa ggagatcgca actaatcgct agaaatatca gcctgtttct caaatcatac
    11521 ggttattata ttcgataacc gtggaattgg aaatacaaca accggtatca aaccattttc
    11581 gattcagcaa tttacaaatg atacagctgg tttgctggat gctctagtgt tcttggattt
    11641 tctgtggttc taaaatagtt caagagctta ctcttatgca tccagaaaaa ggttaacaag
    11701 tttataattt acggtagtga ttgtgatgga aaagaacatg tacctccctc cccaagctac
    11761 actccgcatt cccgaaaatg gattgcagag aattcacatt tctaacgcaa taaagtttgt
    11821 gtctaaagaa attgatccag atagtatagc gggacagctt ggagtgagcc tggtctccat
    11881 gaaatgtaac gttctcttca tttgctgagt agtaataggg ttgactaatt cttattaaca
    11941 tttttgatag tgtcgttttt aatagtgttc ccaacagagt ctctaaatcc caagaatcat
    12001 cgtaacgaac accattgata aagaaagtgg gtgtaccatt tacaccatta cgtactccgc
    12061 taagaaaatc ctcacggaca cgtccttcct ccgcacgacg aatctagctc tggcatttcc
    12121 acaaaatact atagaggtta tggagagttt tttagagttt tatttattaa tttagctaag
    12181 tcaaataata aaatattggc attgatgact cgagctgagt tttatggaac aattaaacta
    12241 aaaaaagaaa tgaggagaga aaaaaaggtt ttcttcaatg acttagagat atttgcttaa
    12301 gaatgtcaac gtcttttgcc acgcatcagc agtttgttgc ggagcataat tagctccagg
    12361 aggatttgca aaggcatgtc ctaatccctt gtatatgaga atttcatttg taataccctc
    12421 gccatccaat gctgctttga atgcgtttat gagtgataat ggattagctt ggtcatggtc
    12481 tccaaatatt cctaacacag gccactttat ctttgagagt tcttgtttat ctataaccaa
    12541 tggagttcca tagtacaata tagtagcggc aagagggtgt tgttcactat gcaaggctag
    12601 ttgcagtgat tgcccgccgc caaaacacca accaaaagaa gctattttag aactatctac
    12661 aaaaggaaga gaacttacat attttacggc tgcttgtagg ttagaaatag cttcttgcgg
    12721 gttatctcta actgatttta ctaactgcga agcttgattt ggatcgttgg ttgattgccc
    12781 cttaaacaga tccactgcca atacaacata tcctgcttgc ttagcaagag tgcgtgccat
    12841 gcttcgtatg ttatcattta taccccaaaa ttcatgaatc ataattactg ctggaagttt
    12901 cttgcctgta gcggcggtac tagaattgcc attagtagca gtgggagagg gatataccaa
    12961 gtagccagaa gcattgtcaa agtatttgac ttctttatta atttgtaatc cctctttagt
    13021 ggcagtagca gttgaatttg agattgtatg gttagccgta gtttgtagct caactcacag
    13081 g
//
LOCUS       DAOR01000004            4570 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_41905, whole genome shotgun sequence.
ACCESSION   DAOR01000004 DAOR01000000
VERSION     DAOR01000004.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_41905"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1278 a          959 c          979 g         1354 t
ORIGIN      
        1 tggctgttac cacttcagca atatcccgtc ttaacttgtt tagcatttca gcctgtttta
       61 tgtaagaata gtctaacgct tgtctgggat cttcatgttt atccagaata atgtttacct
      121 tttgccttat gacagtgtaa atgcggttca taagtcccat attgagtgag gttgaagaga
      181 tctcgatttc ttattaaatg tgacgaagtc caaaaatctt tagctgttgt aaccgaacct
      241 gcgtctttcg tgttcatcag gcgggccgta atcctccttc tttttgaaat aaccccaata
      301 cagaaggatc cctccgccga tcagcatgcc tataacaaat ttctcgttgg ttatctgtgc
      361 gtttgtgtaa ttatcaagat aaaaggcgaa ggacaagttg gtcgtaacaa aataaagatt
      421 tctaagggcg ataatggctg ccatgacaat aaaaagtatt cccattgccg acatccagtt
      481 ttttccgcgt ctacttcgtt cttccccttg tcgaaatttc atggttctac actattcgga
      541 tccctgggtt cttgatctta tttctgtgag ccgaatttaa tagcatggga gttagaattt
      601 ctgcctgata tgaaatggat aatggcccta ggtatatagg acgcaatccg gcaatttcag
      661 atatcaaact attgaccgtt gatacaatat tccgatcgtc tccacacaca aaggtgtctg
      721 aatcgaggct ttctgttatg tttttgagtc gggcctcaga gatcgtatga aatgctgata
      781 cgatccggga tctgggaggt aagttatcgg caacgatctc tgcagcaggt ttcttcgact
      841 gttctagcgg aatgtaaaca aatcctgccc cagtcttttc catgggaacg atgggggtta
      901 taacaataca atcatccctg agttgttttg atagctgtga gcacatatct gcaatgtgct
      961 cgtaatgaat tgacaacaga agaatgtcag atgattttgt aaccgccaaa ttttcatcac
     1021 ctgtaatgct cccagtcatg cgattcccgt aggactcttt ggctactttc gcgtatgttt
     1081 ctgctgcgtc ttttgccttt tgaacctccc tggaacctat aattacatca tgcctagaag
     1141 accatctcaa agcgaaccct tcacccatac ctccagtccc gcctattaat cctactttca
     1201 tcctagatgg ttcaccaaat gtttggttat taagtttatt aaaaagagat actcctcagt
     1261 atctactggt atctaaagtt caagtatcat aaggcttagt cgattgcagc gaaaattaat
     1321 attataatcc gcttttgaat ttgagtggtt tacttgccgc tggtcattct tatgcgtcat
     1381 ggacaagcag agaacaatgt caacagaatc ctagtagggc gacacatcga gtcgcacttg
     1441 actgactacg gaagacaaca agtagaagac acggcaaggt accttcaaaa tgttccgata
     1501 gaaaaagtct atgttagccc cgtcactcgc actgtggaga ccgcaaaaat tgtgagcaag
     1561 atattaggaa taagctacga aatcgatgaa cgattatacg agattgagct tgggaagcta
     1621 gcgggtatga attacgatga cattatcgat aaatacggga atttatttct caagttttac
     1681 gcaggagccg atagcatgct ttcgaactac ggtgtggaga gttttacggc cgtgaagcga
     1741 agaataaaag acttgatgga tgaaattaaa gagactgaac acgataaaaa tgtgttaatg
     1801 attacgcatt tggatcctat caaagctgcg atctcagttc tgcttgactt gaaaccggag
     1861 gctttgtttc attggcatat acgcaacgcc gcgctaacaa ttctaaaaca tgaaagcaaa
     1921 atatactcgc tgtctggagt caacgtaatg agcatgcaca ggtacctaac cgagtaggac
     1981 acggcttcgc gcagatattt gtcttggtaa gtaactgccg atatgttgca tttgtatccg
     2041 gctgaccgtt aagatatgat atcaattaca cattgtcctt gtctcgaagg cgttctcttt
     2101 tttttcgcct attcgtcgtc gaattctact acaaaatata taagtccgtt gtctatgagt
     2161 tgtcgtgcta cggcaaacac gggctaagat atttgccgtc tatggaagta tcctggtatt
     2221 attgacagct gcaattatgt cgggaggcgg tcccttgtca ttagtttcta cttccagtgc
     2281 acatctagat catttacctc actacaacgg aggaggcacg cctgtaggca ggttttacgt
     2341 caatcaggca atagaccccg aatatactcc accatcgcag cctgctaaaa ttatcttcag
     2401 tatacaagat caaaatggta atgacgtcta caatgtctat accatggtag agatctatag
     2461 cgccagcaca ggagagcgac tgaaggcttt cccttggaca agagaggata ttggagactt
     2521 tgcactttat tacacttttc agaagattgg taattatcaa attgttataa gtatagccaa
     2581 tgacaaccct aatggcaatt atcccaatgg catagatcct cccagaacta tcttaactaa
     2641 taatttgaat tgtagttgcg atcgaggcgt tctaaacgta tcagtatcaa gaagttttgg
     2701 gaatatcttt agcaccgcag tcctaagcgg aattgtaggg atgattgtag tcttcggagt
     2761 tgtcgctgca ctcacctatc gaagtcgaat caggagtggt gtgcatttag acgcaataga
     2821 caaacaggtc ttaaaatatt cagtcatgct ctttgcaata gcagcaggta tagtacacct
     2881 tgcagtcttt tctgagcacg gttctctccg cctcgaatac agtattttct tgttagccgc
     2941 cggggccgct cagctgtcct acggcattat gtatgtacta ttgactcttg caaaccaacc
     3001 aggatcactg gatggtccag gactcgcaaa ggtctactac aaaaagactg taattctcaa
     3061 tctttttgga ctagtcggaa cttccgtgct gctaggattg tatacatatt ccgttgtgtt
     3121 accgccacca ctttctccaa acaacaggcc tgaagatatt gatcttgctg ggattttgga
     3181 caaatcattg gaagtgctac tagtgattgg aatagtatac cttatgcgtt gggaaaagaa
     3241 aaaactttca aaagagctgg tcgaaatatg atacgataaa cgttcatgca attcctcatt
     3301 ccctttcttc agtcactcca tttcataaaa ctctgaggta tcgtgtttct ctggttcgaa
     3361 cactatagaa ttgttcctga attctataat attcacgttc gttatatttt tggaataatg
     3421 atatttttca atcaaataga tctcacctag gcgtttccaa attgtttaca atagagaaat
     3481 tctcccaacc tgcataaaga aattagctat caaaagcctt aaattatata cgcagagcta
     3541 gaggacttac acacaaagag gaaaatcatg atggcctcga atttaattat ttggcgaaca
     3601 gtgctatttg ctgtgcttcc catcattgtc atattaatcc tgagtgtaac tatatctatt
     3661 gtatacggtc agaccagtgg tgctccccaa aattatgtga tcaggagggt agagatttgg
     3721 ggcctgttct atagaggaat ggtagccgca ttcatcgttg gagctcttgt acagggctgc
     3781 atcgtttatg ttgcttggag gtttagagaa tccaacaaga aaacgcatcc acctagagaa
     3841 tcaccagagg acagtcttcg gtaatgtcag gtcacgcaat atacgaatgg atctatatag
     3901 gcgctgtaat ttccctcttg gcatgggtag gcgctgattc ttggaatgta gagcatatgc
     3961 tagaagctgt gccgccgaat acggaagtta taaaggtgat tggtcagcaa tggttttgga
     4021 ctttccaaca tatagacggc actagagaaa ttggccaact gcatgtaaaa cagggggttc
     4081 cgtatagatt tgagatcgta tcgcaggatg ttatccattc cttcaatatc ccagacttta
     4141 ctgtcctgat ggatgccgtt ccaggaaggg ttaatacggt ttggaatgta tttgacgttc
     4201 cgggtcagta cctaattcaa tgtagagaat attgtggctt gcttcactac aatatgaggg
     4261 cgcagttgtt tgttctaccg gcaacagcat cagccggtgc actttcgcag gtatctactg
     4321 cgggagcggc agggtcgagc aatacagcta atagtacctc tgccggcaac gtcactagtg
     4381 gctcttcttc tacttctgct gttacatcgc cgataggagc caaggtatca atcctagtcg
     4441 gtgcttctac tcaaggaaac cctgcctatg ctcctactcc agcaactgtt aagaaaggcg
     4501 attccataca ggttaccaac aaggactcgg ttccgcatac ggttacaaat ggcaaagatg
     4561 caagcgatcc
//
LOCUS       DAOR01000005           14922 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_7165, whole genome shotgun sequence.
ACCESSION   DAOR01000005 DAOR01000000
VERSION     DAOR01000005.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14922)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_7165"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5329..5496
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6293..6604
                     /estimated_length=312
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4314 a         2822 c         2790 g         4516 t
ORIGIN      
        1 aaaataaaac aaccacatct cttttcgaat cctgatgttt acattttgta ataatcgaat
       61 aggttaaaat attttcatgc gatcgaagag aacgtagaac ggtatttctt ttgactattc
      121 ttatccatga ctccctctcc attaggagat ccaattcttt ttaacagatt cttcttttct
      181 cctctagaat gattaccagc catatttaat ctgtaatgta cgaaattcat ctagccttat
      241 tatattccaa atgtaattac tgctgtaata aatcgggtcc ttggactata acaataatac
      301 tgctttttat aggaattgca attaaataat ttcaactacc tttgacactg tgtttaatgc
      361 atctcttatt agcagtacca ggaataataa tgcgaataca gttatttcag gaatcattgg
      421 tggtatatag ggatagtaat caatgtagtg gtagcatttt atccatataa acgaaatgtt
      481 aaagcgtatt tcggtatagc aataaaatag gccttctctc tagttccatg ggatggcatt
      541 atgtaatgaa ttaatttgaa aatataaatt agagtcattg aatactatat agtactgcca
      601 gaaaatagat tatggtgttt tcattctctc tttttaatca aataaagatc cagctaccac
      661 tttaacatga atcgttatct aattcttact ttaaactaca acaaaaagaa tatacccaat
      721 tgaaaaacaa tctttatttg ccgataagga ttgtcatata ctttaatatt tgggaataat
      781 tttatctctg agagtccaaa gtctatcaaa atttacgaca ggattctctt ctccttaggt
      841 gaaaacaatc taggtcctta cttgagcgca gagatttccg acaacatggg aaagcaatta
      901 atcaaagtag accataatgc atgtatatat tatcatgaaa aattaattaa caaatctagg
      961 gaaccagagc atattttaat aataaacatc aacggtgaaa atatattgga atcaagggtt
     1021 ttagataaaa aaacaattct tgtaagtggt atgtttgctt tcccagaatt tatattgatc
     1081 gcaacacaga attatatcgt gatgcctagt ggaaagaggc tcatgcacag tcgagttaat
     1141 gcaaagaatg gtgatgtagc aatcacagaa gatggtctga aattagtcta agctgagttg
     1201 ctttcttaca aacgcaggaa taatactaat aacaaaaaat tatggctcaa aaaaatctaa
     1261 tcggcattcg gtcagaataa tacgacagta gatgcgaata tgactgcacc gtccgaattg
     1321 actggaacat actctgcaaa taacataact caaatgaaca ttctatttca gtcatgcaat
     1381 gatcttgtta gtcaaaatgg tactttaacg tcagcaggga gacagcaata gaatgtatta
     1441 agaatggatt gtcgttggga cttgaggctc tacatcacgg agtgccatta tggagagtga
     1501 tattcagtct tggtctgctt actcaacccg gcagcgtagt aagcatgaat gcaatagaat
     1561 cgtgtgatca gttccaattc cttacacacg cgatgggtct acaatagacg cgaattcatt
     1621 acattacata ctactgatac gggcgttgtc tgtctggtgt tcacgaggtc ggcaacagac
     1681 ctaattggtt atttattaat tgaacttttt acagggtctt tatagtctta cgataaccga
     1741 agtttgtttc ggactaaaga gtctgatcat tctaatttcc tgttgtaagg acagctcgaa
     1801 atcgtacctt accgctcatc attagctcgt aggcctctgc tgcacgctcc agtggaaaga
     1861 tttcattcat ggaacgcacg ccagatagca gactgaagga tagcgtatct tgcgaatcga
     1921 tagaagatcc gctgggccag ccgactactg atctacgtcc ggagaggaaa aaattcggag
     1981 acacttcaag cggttcatca gatgctccaa tcatgatggg ctttccatcg acggccagac
     2041 cgcccaacac tgcgctcatt acttttccgc ttgggaccgt cgcaagaatg atcttggctc
     2101 ctccgagctt gacaagttct tcgataggat tctgcgattg gctatcaata tattgtattg
     2161 cgccggacag aatacgatat aaagctcgta gttgatttca aatgaaaacc gagtagactg
     2221 cctttgcctt cttgatttct tcattcgcgg ccgatgaagc agcaggaaag tggaaagaaa
     2281 agctggcacc ttcaatgatg tacaaataat ttcatgagat aaaatcttga acacatactg
     2341 tatcaaaatc tatccagata ttatctctag taaaaatgaa aacctaaaag tgtagatgtg
     2401 gacggaatgt atatcctgtc atcccgaccc tggctatctc ttgcccttcg ctgacagctt
     2461 gacctacttt gacttttgag ctttgaggtg ccaagtgatc gtacctggaa tattcttcat
     2521 tcgcttgcac gatagttaca aaatttgtaa agtccaatag attgggttaa gcccaccaat
     2581 gctagaatcg tctttaagat aggtgactat accattggct gcagcgagta ctggagtatt
     2641 ttctgggact atgaaagcta ttgcgtttcg aaggtttcct gcgtgagctg gagaggacgt
     2701 tctgtccact ctttccaaca aattcctcgg tacaggtaat ctatagcggt taatagcact
     2761 cttccaaacc tcttaaagtc tccagcttta gagtctcaac tttgatacat taatcgaaca
     2821 agaaaggctt aggaggcttg gagcattgga atcttcatgg ccagatcctc ctgacatctt
     2881 ggaatctttt tctcaacgtc acgatacttg tgttgcctgc aatagcgatt tgagcttggc
     2941 tgaccttatc tccttccttt agacaggcgg catataatgt agcaacagca agagcatttg
     3001 gatctttgcc atgtgatatt gggttctcct ttattacaga aagcatttga agtgctctcc
     3061 tgtaggcctt ttcgcttaca cgagccctat ttgctatctt ggataggtac acgttcgaat
     3121 cgattactgg cagtttaagt tttagatgcc ttagaagtaa cctgtaacac ttaccagcga
     3181 atatagagtc tgcatttgca atctccgcaa tttcatcaag tgttcgtggg acattcattt
     3241 ctctgcatgc agcatagata gatgacacta ccaatgcacg aattgatcta cctttgataa
     3301 tttttttgtc cagagcctta cgatagttat aagcagattt ttctagaagt gcgtcgttta
     3361 atgaaagctt atcttttaca ctgcttaaga tagcaaaggc acttttcaaa tttctgtgat
     3421 agcttctatt attgttagaa atcttattcc aatgtctcat tctctgaact ttactaatct
     3481 ggtcagggct tatcgcacca ccgtttgcat ccacatttga ataggagata aacgttgaaa
     3541 gccccatatc gtgaaacgct aaagaagttg gcatacctgt ccggctcttg ctctcgatat
     3601 cgtctcctga gtatgctttc cattgagggt ataatgactc tgctttatca gtgagaacca
     3661 tgccgcagga gctacacacc gtctcgctag tttccacgtc gaaaagaata gccgtactat
     3721 tgcacatggt acaaacctcg agattgttcg gggaagcttc catctgttgt cttcgttctt
     3781 ggtgttcgcg cttaccttcg tcagcaggag aaaacatcag aggactaatt gatttagcca
     3841 ggacaacgtt ggctgttgac gattttgcca tggacttacc actactgaat aaataataag
     3901 gaacatgtgt actaagcatg gtcggctgaa cagaaatcaa cacatataac tacaaattaa
     3961 cacagggaag accttataat tgcacttatg cgcctgtcat gatcaaaaga aacgacgtgg
     4021 attatacggt tttcgtggta tatacacgtg agagattgtc tacaagttga tccatgaccg
     4081 cctttattgc aaaggctggt atgcaatgtt tactcgctta atatcaagat agcagcacag
     4141 agaacgcggc aggaactaaa gtatcaagcc gaactgattc tattagagaa tatcaagata
     4201 aagagccaat gtctccggca aagctaaagg aacatgaacc aactgctgta aaaagagaga
     4261 tgacagaaaa gattatcgaa ggtggtaaaa catcaacaga tctggaaaac gcaaaagagt
     4321 tagctagaaa aagcgggatg gcagaaggaa cagctggcgc tgctgagggc ggcgacgagt
     4381 acgaatagga taacaggagc aggtacaagt aagtagtagt ggcggtgcta gtaggattgc
     4441 ccaatatgga taaagaaaaa ataggcgaag caatcgatct agaaatggct gcacacaaag
     4501 cagttgagca gcttgcttcg aagggtttga tccctaacca gggatcaatc aagttagaag
     4561 gaatgagaaa agaagccata accatcagac ccagatggag gatttggtta gaaagctatc
     4621 ccagtccgac gggttagact ccgcgaagat tcaggagatg acacaggaga tcgagcaaaa
     4681 agcatcaaat ataatggaaa tctatcttgg agaggatcca gattctcaag aagcattaga
     4741 atttctgtgt ctcgcagaag gtggtgaagt taccgattat gaggtcttga gtgcaatgac
     4801 gaaaggtatc aaaaataagc aattcccagc aaaggtaagc tctatactca accaagagaa
     4861 aaaacaccta cttttgtgca ccaaattggc taagaaaaat gctgctgcac cttcgaaata
     4921 gttgcgcttt tttatttttt tcgaatcttt tccaagcctt agataaacct tcttttgaat
     4981 gcaatatcaa ttccgctcat tgttttgaca agttcttgat aatccgttac gtctgccttg
     5041 actatctgtt ccctccaata aagccacttg cacatgtccc tagaattcgt atttgtttga
     5101 ttcttgatgt gcattattgt ctatatggct tgcatatccc atccgaaatc gccataatcc
     5161 cgaacatgaa agagaacatt tattgaatac attaggtaac ctgaccagtt ttctgtaagt
     5221 tcattgtctt ggagcaacca acttcaattt agtttattat ttgtatacaa gttgcggcaa
     5281 tgtttcccgg gagggcgatt tactttattt ttgtattttt ttacaaaann nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacct ccccggatga catgggtagg
     5521 aataagagaa gacgacacta aaacctgata tgtattctgg aattatcaga actatcctcc
     5581 cccgcgctgc atataatcaa atcaccgcca atagttaaca tctggatgcc cattatgccc
     5641 taaggagaca tatgattttt ctctcctacg ccaactgtgg cttgcgaagc gtgtcctcaa
     5701 gctgctcccg ccagcgaact accttcgatg atcgaatgag ctcataagga gcggcacagg
     5761 agacacgagc ggacttcgag gagaaattga cgcacctatg aaaattctgt ttgtcttatg
     5821 ttgatcgaca gaaagtacaa tgcctaggtt gtcaaatgta tgagcccttg ctctttataa
     5881 acatgatttt catacctttt tgtcgcagat gaaaaacacg ctatgtggcg atggcgttgg
     5941 agtaaagaaa tgaccgatcc aacaatagcc aagtacgaaa atgaaacacg tcaaataatt
     6001 tctatgaagt ggatacttgt gactactata gttgctggga cgctgctaat gatgttgtcg
     6061 atcgtagcac cattcattgt tctagctttc cttcatcctt tgtccccctc gttgccccaa
     6121 caaaccattc taactgcggc ccagcaattt acaaccgcac ctagggatgt tctaccgttt
     6181 gcaacagctc tagtaggatt tgctggtgga gtagttactg ctatgtatag gactggtaca
     6241 gcacaaggac cgcctcctca atcatcacct ggtcaaggaa cggctggtca agnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnggtcaa ggaacggctg gtcaaggatc acctaagatc ccgtgaattt atcctatctg
     6661 ctgatatagc tttgacgagt tcttccaacc atgcttgttg catgattcta atctacagat
     6721 tctggttgct ggcattcctc ccgggagggc ggctgcctta tttttgtatt tttttacaaa
     6781 aagttttaaa aagtaatgca gcctatgagc gtgcgaatga tgcctcttga gttttaatct
     6841 gatcgtatta aaaattgcag tttgacctcc ccggatgaca tgggtaggaa taagagaaga
     6901 cgacactaaa acctgatatg tattctggaa ttatcagaac tatccttagt gagtagtaac
     6961 gttggcatag attttacgga aataaggccg ccaccttggc agggacccag acgggtatgg
     7021 cacgagtccc atcaaagagg gtatggtgtc gtaggtgaaa aacaagaaga cctgataacg
     7081 ataggatttc gtgtcaaagt gaaggaaaag gcaatcattc aggactatgt taacaggatg
     7141 tacaaccatt cgtagtagat cccaagacag aagaacagaa gagaatgttg gattatccct
     7201 ctagcgaact tgtgaccaaa cttgtaactt tttcatatat gactcaatct aattgattcc
     7261 tgctcgctgg aacttttctt ttcagaaatc atcatttaat gtaagtatcc gaaattgtcg
     7321 gtagcatcaa aaccaagaga gaatttaaat tcaaccttca catcctctca atagctcaat
     7381 ttcggctcaa tcgactctta gatttggcag catttttata aatgctcact cgtcagggga
     7441 gaaaccaaca atctttgtca atctgaagct tgcattaaca aggtatccag cggcaaccac
     7501 catatataaa agaaccatgt gaagtcctca gctagcatag cggatataac gttagctact
     7561 cccaaggacg cggctttact cctttcatgt gtaccaagta tattaaaact aacactatca
     7621 aagaaaggcc cgaaatctac gagaataaaa attaccatca taggatatat gtggtaagaa
     7681 taaagtcccc ctgttattgg agtagcaaca cgatgagtcc caccgtttac tgttaggttt
     7741 gcagtttttc ctaggactgg atcattgata taataatatt ctagataacc gtagctagct
     7801 gcaaatacga ttaccaagct agaagtcaca agataagaca cacgtaccct tttcagaaga
     7861 acgagaaacc ttttcctttt caactctttt ttttaacagt tttcttatct gtgtatttta
     7921 ctaggatata ggtactagta cttactctta tctattgaat tacctcaccg cattatcatg
     7981 ttgatacaac tgaaggcaaa ggctcgtagt attgcatttc gagacattaa ggaaatgtta
     8041 ttcgattctg gtgacaccta gagctataga gactgaatct atgctacaag caatccagtg
     8101 gctagttcta tttttgcttt tgttgtttct ctattctctg tgtaagtatc aagagttgaa
     8161 tacaagaaat ctgcctcctt gcctaatgcg atcagaatat tgtatgtggt attctctagt
     8221 cttgttgttt ctatcataga tagtactgtc acaactgaat taattgagta tcgatatcaa
     8281 tacacaatct gctttatgtc ttctggagaa ataaatcgtg aagcattaag agagatatat
     8341 acagcagtaa gccaatgatg ttttatcaaa aaggcaggta caaccgatca tttcattaaa
     8401 agagtatggc agagttggtc tcagtattat ggtgtcttcc acaggaccaa taatttagta
     8461 gaaacggtaa gagctgaact aaagtaaatt tttcatggga tgaggattat agctactttt
     8521 aagaataaga aatggaatta gtaggtagtg agtagtaagt gaatactaca ataataaggg
     8581 ctcatacgct ccggcaccta tctactaaac attttatcag gtagtcccaa aatgaataaa
     8641 actcgttcat acggacgata aattcacgca attaaaacag atgatcttgg cacagtctgg
     8701 gacatttcta cttttaacgt agaaatatgt ggaaaaccag tcagggtaat atggatctcc
     8761 tgggactcgg gtaggttagt gacacagatt agcctactca acatattgta gaggaattat
     8821 tctttgctct cggccacgaa aataatttca tacggttaaa cgactgtcga agactaatta
     8881 attatgttta taggaaaaat aaaaaagcgg aataagaaaa ttattcctct cttatcactt
     8941 caaagtactg caaatcgccc ttgtggatat tctcatcagt atccatgacc attcagtcct
     9001 tgaaagtctc tgtgagcatc atcacatccc tgtgttgcca tgaacttcta taaacctact
     9061 ctctgggcca aagataatag tcaacactct gtccgtattc gtatttttcg tttctttgaa
     9121 tgtgttgtcg atattaggac acgttccaac acatatgcct attggctcgc acaagatgat
     9181 gtctaccgct gctgggtgag tgcaagacca aaatcgtttc aatccgtcta gtttagtttt
     9241 tcttattatg gtcgtttaca ttaaatcaca ttaaaatcct cctagaaagt aatttctgag
     9301 cttctttagg aagtacaagt tgctactgaa tatgcttatt cgtattagtt ctacgttaga
     9361 gtgcaataaa ctacaacaaa atatctattg gaatagacca aacaacagca ggaagtgtta
     9421 accttgaacg atatcttacc taagcgaggt aaacgacttg gagagcaaca acaacaactt
     9481 gactaggtat gaaataggca gagcacgaaa aatatcataa ctctggatgg agatactttc
     9541 acggtcacta ttattattgt acaggatcta agtatggaaa cacaggcgtg ggcaggtcaa
     9601 cattgcaggt ttgtgagcag aaataatact ggaggaagtc aaagggcatt tgggtgttta
     9661 tcctacttcc aaaatagacg aaagagaaat tcctcctcca atttgacata gctaaagcaa
     9721 aaagtatcaa aaatgcaaag acttttgaaa gcctctaaat gattttatag gctttactga
     9781 ttaagtttat gttaggacta ggaagaagac atgacagagc aacaagctga accaacaaca
     9841 aagaggaagc ccagtccatt acaaaaagag atccttgcaa agataaggaa gttagataag
     9901 gtaaggattg tctaatcaat taatgcttta tttgtacggc tgctattatg tgtaagtttg
     9961 ccaaaatgtt ttgtaaatac agactaatta agtgtaggtg ctgatttgtt gcgttcaact
    10021 caatagtcat attgtaaagt ttatctgctt cgttttcatc gttatcttgt tgcaatgtct
    10081 gagtcaaatt ctccaacaga gcctcgaacg agcttccgta gtagagcgat caaagtaatt
    10141 gctcacatta tagccctagt aggcactcta ttgacaatat ttactcaaat tggtccagcg
    10201 gttgcccaat ttcaaagccc tcttgtcaac atgcagattc agatttacga aacgattcgt
    10261 ataaatgaaa tgtatgataa acaaatcata agacgacggg agaaaaaact aacacaaata
    10321 caaaaacaga tggttaatac aatcagatcc ctggatcgtc gcagcatcac attttgataa
    10381 agtctaggag acgataacaa tgaatagtat caaatgcgct tcctgcaaaa taattaatgt
    10441 agagaatctt caatcaggta agattttaaa caagataact tctgtgaaat tgtgcgataa
    10501 acacaagaat gtagttgggg taaactaatg atttttaaaa ggtgtaagat ttgttttaga
    10561 tttattgatc tgaacgtagg tgagatattc aatggaaaat actgtcatga atattctgat
    10621 tggcaggata ttcaaaatgg gatgagtttc tggatatcac cgacttgcta gataattagt
    10681 ttgtagattt agaatagtat ttataacctt ctagaaggct atctattcta gattttattg
    10741 acgatgttaa tgatactaac tctgatatcg tcacttcttt tctatattta agaacacctt
    10801 gcatcaattg tttatacgtt atatgatcgt agtcgccgtt attgctaagt ttgaatattg
    10861 tgttttcatc gtcatctata ctttctagaa atgctttaac gttaatgata cctttattat
    10921 caataatagt cattctagat caatcaaatt ctttagtaag tatctatgac aattttctcc
    10981 atccttctca tgacataata gtgtaacgtc agtaacttta cttttctcac gtaactcatg
    11041 tatagcttgt tgactaactg ggttactctt catctcttta tgataaccag atgtgaatgt
    11101 atcccaatca atcattctag ttagataaga ctttaacaat ggagcactag gagataattc
    11161 ctgcatccaa cggttgtaac tacctttctt aagaaatctt ggacgatgtc tcattactac
    11221 tacacgatat ccatcgcttg cctcagaatt atcatagaca cgttttgttt tgatggtcat
    11281 ttcttatatt tcattatagc agtcatagca cactttcgta ttacaacaat cacacttcat
    11341 acattttaga cctgacaaat ctttcatgca ataggcacat ttcgtgaata atacttcagt
    11401 catttcctat ctaccaagat gttgtttaca agagtcgcat aaaaagtaac atttaatatt
    11461 actcggattg taacagtcag gacttacatc atatttggtt agttccatat ctcctttatc
    11521 acaataatca catttctctt gatttgctgc tgttggtaaa gtcatctgtt cctagccaca
    11581 ttaagttttt tttgtattct tcttcgtttc tgttccgctc ttacatttaa taaataagtc
    11641 atttcaggcc acctgactta gcgaactgca gacaatcaca gtcatgtttt aaacaaaagt
    11701 cctttccgaa tctgtgatca cactgtttat gcgagcacac tgtttatgcc agcacccgtt
    11761 gcacttattt ttaccctcat attcggcctc tgtttgtgct attgtcggta gagtcatttc
    11821 gtggatttac ctgccttctt ggatttcgct ttctttgctg atttgtttgt catttcttat
    11881 cccccttcac tttcatatac gattcccact cttcttccca tgtaaaccca caacttaggc
    11941 attcgatatt acacatataa ccagatacgc ttactccttc tgctttcgga ttagagcgcg
    12001 taggacacat tgtatacgct actacgtttt tcagacgttt ctcttcctgc ttcactgtag
    12061 ataaccaagt tgtcatttct tctgttcctt acatgtcgag gcctggtgct tttctaatga
    12121 ctctaatgtg ataaactcgg aattacaacg tttgaagggg aaaataattg aagtcatttc
    12181 gtggatttac ctgccttctt ggatttcgct ttctttgctg atttgtttgt catttatttt
    12241 tcaactcctt atgtgctaat tccaatgttt ctttagcttc tttgattaac gctttaaggg
    12301 tttcgcgatt attttcattt tctatttctc tagctcctta ttgactgcat tcaaaatatt
    12361 gttgtatatc tgctttctat ggtgtgtatt tacgtagttg tctttcttta ccaagaagct
    12421 cataacaagt ccatttatca acataggcag cgctaattca tcaaacttgt agtaatcatc
    12481 ttccgtttgc gcctcatcta gagcactctg caatgtatga attttgtcat tatgtgacgc
    12541 attcaatacc attgtcagtg ttttaagctc ttcagcatat cgtagcttcg aatacaaatc
    12601 tggatatagt tcttgaaact gttttatgat tattccagct aaacgttgaa gctccttgcc
    12661 aagagagtca ggttcgggtt caggatcacc tggaggaaac caaaataatt catttcctct
    12721 aggtatcttg ggaatctcta aagtttctag ctcacttttc caattggccg ccgcttctgt
    12781 ggctatagcc tctgtaaact cttccagtgt gaatcctgca gtcgttgcaa cctgctgttt
    12841 tatcatttca taaaactctt cgtctgatct agtgatagtt cgtcgttcct ctcctgtaca
    12901 ttctctaaac ttgttcatta ttccatcgaa aaaagatcgt gcagcaagct tatcgtgtgg
    12961 tagtgttctc atactaggat cagtcgttac aatggtctgt aacatttcat ataatgtcct
    13021 gtcttctagt ttcttgccac tgacctcctc taccacatct tctattttct tttttatgaa
    13081 ctcgttgctt aatccgtcat tgtatcctgc agtgaagata atgtcaatca tagctttctg
    13141 tgtttcaaat accttatcgg ccgtatcatc ctgtttaagt ccacccgctg tcatgatctc
    13201 cttgtactcc ttcaacgcac gcaagaatat ctctgagggt tctttattgt tgtcttctgt
    13261 catttctatt tattacacac tcctaccttt ctagatcctc ttgctcttgt tttatcatat
    13321 actgaatagc ttcttcgtga atggcttata taacatcgaa tgatatctgg aatttattgt
    13381 aagcaaacgc caatcctaaa aattagattg tttgaaattg atccatcact cgtctacctc
    13441 agaggataat tatctccacc cgcacaatat gtcgtatcta tttcgcataa gtagctaaga
    13501 atctgtgagt caatattggc taggtttagt ctcatttgtc gtagagttta ctggatgtga
    13561 agtgtcattt ccaatttcat caattgcgac ctgagctgct aacattaact tataggcttc
    13621 aattattgtt tgtatgatct aagactaggg gagggttaca ggttgcagga ttcgatgatg
    13681 gtgtttgtgt cgttgacgtt gttggttgtg ttgctgaatt ggccgtgttg gactgttagt
    13741 atgactcgtt gaagaaccag acgatggtga tatcttgata gcgtaggtgc tgctatcaac
    13801 gaaggtagcg caagctagaa gaatcagctt agtcatttat ttcaccttga acccatattc
    13861 aggagtaggc gtcgttaagc aatggtcggt aatatttcca tacttgttta tggcagccgc
    13921 gctattattt ccgtccaaag caacacttcc cattcctagt ataagccctt cctctatgca
    13981 tgtctaagtt gtggatcagt aagtcccgta agtatcctac cagtaggaac gtgaacaagc
    14041 gttgaagcga atgcgttcaa tggtgctatt aataaaatac caaatactgc actaatgcca
    14101 ttaagaaaaa gaagatttgt catttgcaga ggacacggta gatgcgaatt agcgacgact
    14161 cgtattttcc aggtgtttac aaacgcacat tttatcgtat agtttatcca ttattccaca
    14221 ctcctatcta tgagttgcag ggaaacttta cgccgcaata caagttattc gccttttgat
    14281 atccagctac acaagcagtc tggtttatca ctccttgtgg ttgtttttag attgagttga
    14341 ttttacagtt gtttaaaata gttggttggt ttatggtgaa ttgctactac aactgcctgg
    14401 ttgcgattga ctaaacgtag atgagaaccg ctgatctgct gaggtgctag agcaacttga
    14461 ttgagagccg cctgaactac ttgaagtagt caaacctgcc ccagcatgga aatctattga
    14521 acaagggggg gcagcggatg gagcgccgca acctattata ctagcatgta ccatgcctga
    14581 actacttgaa cttgtagagc agctgccttt gctgccgctg acgcttgtag aagcgctagg
    14641 tgtgtgcgct gcggaggaac gactgctaca actatatgcc tttgcgacgt ttggcgttgc
    14701 gtttaaactt actaacaacg ttgcgaaaat ttagcatttg aatatgagaa gtgtttagtt
    14761 gagataattt caattcagaa agaggcttgg gcaatctcta aaacgtcaaa gtatgagagg
    14821 aataagataa tgggaattgc attagcaaag gatgcattag cactaaagat tgatctatta
    14881 agcaatattg atactatgga taaggcgata aggtttgtta ag
//
LOCUS       DAOR01000006            7863 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_12311, whole genome shotgun sequence.
ACCESSION   DAOR01000006 DAOR01000000
VERSION     DAOR01000006.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_12311"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2436 a         1477 c         1542 g         2408 t
ORIGIN      
        1 ttacataggc gttataatag acagtccaac aattggtgtt gcaaaaagca tcctgtgcgg
       61 ctcagttaat gcagatcatt ttatcgatgt tgatggagtt atcagtgggt ttaaaatgat
      121 aaaggaaaaa aagaaaccaa tctatataag cgtcggtcat aaaattaatt tagtaaatgc
      181 tatcagaatc gttaagcaat tagttaagcc ggaagaacga attcccgaac cgctacggct
      241 tgcagatatc tactccaaag cgctagcaaa ttcggtacct taaacgtaac tcactacaaa
      301 tcttaatttg ggttccatca agcttagttt atgtgagcac aatcagaacc accgacagca
      361 ttggtttaat tgaaaatcac ttggtacgca tgtagtagac gaaacaaaat gagaaactgg
      421 actaaccgaa gtgttgctgg cccttctgaa gtctctacca tccattctta gtaggttttt
      481 agaacagaaa tatgaccact ctcggaaaac tgcgaatttg ggcactggca tatgttagct
      541 acttctgatc ggaattgttt ccgttcgtcc ttaacttagc gtatgaaatg ctatgggagc
      601 ttaaaatcgt gtcaaataat tcacccactt aaataggtga cttgattata ctttttactt
      661 ctaatgacta ttcttatagc tgcaatgcag gatttcctcc tctcacttga atcatcaaag
      721 acaggttgga aaactcatga gtctctgaaa ggaactcatc cgcaatccgt aacatttgac
      781 cctcgaaatc caaaccgtgc ctattgcggg acgttcggtg atggactatg gaaaagcgat
      841 gatggcggcc aaacttggga tagaattgga aaagacgcta tatccagtaa taacgtgatg
      901 tcagtttcag ttagccgcct agaacaggga aataaagtat acgttggaac tgagccgagc
      961 gctctgtata gatctgatga cgaaggatac tcttggcaga gaatgagtac tcttaacaat
     1021 ttggagtcat ccaaatcatg gagctttcct cctagacctt ggacttctca tgtacgttgg
     1081 attgaattag acgtaaacat ccctgattat ctttttgttg caatagaagc cggagctcta
     1141 gtccagagtc gtgatggtgg cagaacttgg atagaccggg ttgagcaagg accatatgat
     1201 actcacactc ttgctacaca tcaaaaggcc cctaaatgcc tctactcttc tgcaggcgac
     1261 ggctattttg aaagctttga ttatggagag tcttggaaga ggccgacagc tgggcttaag
     1321 cataagtacc tttacggact tgctgttgat ccggctgatc cccacactat tgttgtatcg
     1381 gcatctcaat cggcctggca agcacattcc gttgaaggtg cgaattcatt agtatataga
     1441 agatctttgg agaatgatgg tggagagtgg aaagcaattt caaatggtct tccagaatca
     1501 caaggaacaa ttgttgctat ccttgccgca aatccaaaaa atagtggaga attctatgcc
     1561 attaacaacc gcggagtatt ttgttctatt gatactggcc ttgcctggag agagctcgat
     1621 gtatcatggt ccaaagaata tctttcacaa catccttggg cattagcaat tcgaggagaa
     1681 gactagtagt tcgcaaattg gattattttt taaccgtaat gggaactatt gttaaacatg
     1741 gttctagata cataagctaa ttgtaactgg catctgaatc gagtcaaaaa gttaaaaatg
     1801 tcagagccta ttctaaaacc atcaaggctg aggttagaag ctgttgaata ttagtagagt
     1861 tatatctgaa gagatttcag gcattgactg gcaaagagta acagaagaac tgaatgaaaa
     1921 aggatacgcg ttagtgtcac gattcttacc ggttcaatat tgcgatgaac ttatcggcga
     1981 atatgacaac tccaatctct atcgcaagac cattacgatg gaaaggcatc gtttcggatt
     2041 aggtgaatat aaatatttca aatatccttt accggatcta atccatactg ccagaagagc
     2101 gatttaccca aaacttgcac ctgttgcaaa tacttggatg aaggtattag acataaaaag
     2161 acagtttcct gatcagtttg atgaatttca aaggctttgc catgacaata atcaaacaaa
     2221 gcccactgtc cttattttaa aatatggtaa gggagggcat aatacgttac accaggattt
     2281 atatggaaat attttttttc cttttcaatt ggttttattc ctgaatgagc cagatgatga
     2341 ctatactggc ggagaatttg tattaacaca gcacactcca agagcacaat caaaagccat
     2401 tgtcttaaga ccgagtaaag gagatatgct gatattgact acaaatttta ggccagtgaa
     2461 agggagtaaa ggatattatc gggtgcaaat gaagcatggt ataagtgagg tacacgatgg
     2521 agaccggcat acccttggaa ttatttttca tgatgcccta agctaattcc atgatacgac
     2581 ataacgagac tagtgatata gaactgaaga atcgaattaa acgaaacgag atttgctttg
     2641 gagaaaatgt aaagcctaga atttacggca aactgaattg tagatcaggg aatggaatgc
     2701 gtgtgttttt tctgctacga caggaagccc tattgcaggc actgcatgat aatggataat
     2761 gaataaaaaa atggatttat ttgatcaaga caggataagc aatcttttac ataaagacgg
     2821 aacggtaaac tattacagaa acgtactgac acatgatgaa gcaaaccgct attttgattt
     2881 gttattgcaa aacatattat ggagaaacga tgaagccgtc atttttggta agcatatcgt
     2941 tgcaaagaga aaagttgcgt ggtatggtga ttctgactat ttgtatactt attcaaatac
     3001 aacaaagcaa gcattaacat ggaccaaaga actttctgac cttaaacaaa gagtagaaga
     3061 cgttacagga acaaaattca attcctgttt actgaactta tatcacaatg gagatgaagg
     3121 tattgcatgg catagtgatg atgaaaaacc attaggagag aatagcatca ttgcctcatt
     3181 gagttttgga gcagaacgaa aattttcatt taagcataag caaactaaac aaacaatttc
     3241 agttgtactt gaacatggca gtttgcttat aatgaaagat gctacacaaa cgaattggct
     3301 tcatagctta cccaagtcta aaaaaataac ccgaccgaga ataaacttaa cttttcgaac
     3361 gatagtatac taacctatcc caaaagacca cctatagtga cattgacgct gtgaagtttg
     3421 cctcacgact tgtgcattta gacgattaag aatcttattt taaggtctat tcaggagctt
     3481 tgattaagtg gaaattaatt cttcgttatt tgaattttga atttacatca tttattatat
     3541 tgggtgctgc tgttttagta gatgcagcat ttgtatttga ctctactgct ttttttgtac
     3601 tctctagata tgcaatgctc attgccaaat ttccaaaacc cattgaatta agattttcag
     3661 acggaataag aactatcgta ttgccctgaa gctttattga ttcatacagc atgtttgatt
     3721 gtcttaatgc ataagctata ggattatttg tgtatacgtt ggctgcctct acgaatctag
     3781 cggctaccag aatctctgat tcaccgtata tgacccttgc gtttttctct cgttcagctt
     3841 gtgcctgcat tgacattgca ttctctaatt cctgagggat gattacttgt cgtatttcta
     3901 ctcctgttac ttcaataccc caattactag cttcttgcgc tattacagat ttaataagat
     3961 tatttatatc aacccttttt gatagtattt cttgaaagaa agcagaacca atattgtttc
     4021 tgagggtagt ttgcgaaacc tgctgtatca tctcattaaa gttttcaaca tttaagagag
     4081 catctttaga tcgatcttca ataattttgt acctaagtat tgcatcaata gttacaggaa
     4141 cgttatctct tgtaagcatc ctctcagctt taaattctcg taccttttct cttatgtcta
     4201 gcacaagaac actatctgca aacggtatcc tagtttgaac tcctggacca actcgtttct
     4261 gatattttcc taatctgaga attattgctc tatcatactg cttgatgata attagaaatg
     4321 aagaaataat tacagcaatg ccagcggcaa ttgctaaata cattatcaac acgtttttta
     4381 atgataatcc agttccaatt ccaattatta gaattattat gccaacaata agttgggttc
     4441 ctgaaccctt cttcataaca tccgtaatac ctggtatgga cgattcccta gacatattat
     4501 ctatatatta tttgcacaac aaccatgcat aaaggtttgg atttgttttt tttagtgaca
     4561 ggtgggatta taaatatatc agagaggact cccgaaatat cgaaaatgtg gatgaatcta
     4621 cgttttggct ctactagagt tttgccgtag attcatgcta gctgtaaaca actttcattg
     4681 ttcaatttct aagatcgata taataccata tattaaatca aaacgagatt aatccctatg
     4741 tggccaagta ttgtcatgct tatcaagttc agctagccgt ttgtatgcaa cctcgactgt
     4801 cataggatca ttatatatgg atgaacctgg ttgctgttta cggccacctt cacgtagtga
     4861 acccgcatct acaggcgtaa aacctatctc atcaataagt tttgatacaa cagcttttgc
     4921 atcggggtcg tctcccgcca caaatagtac tagacgctct tcctgggagt tacgagctcc
     4981 tgtacgcaac atttcataat acatcgtatt gaatgctttt acaagacgag cactaggtag
     5041 ttgttttgtt acctcttcac ttgacgtaga gttaccaaga tcaattactt caaaattctc
     5101 agaataaggg ttcattgcat caattacaat ttttcctttg aaaagatcag atggtggaag
     5161 ttcatgtttt ttcctccaag gtattgcaag taataccact tgagcaaacc tgactgcccc
     5221 ctccggagtt gcagcagtaa cattattgga actgattgac tttacaagtg cattcaggga
     5281 tgcctgacca ctagaattac tgatttctac ttgatgcccg gcttttgcaa ataaatgtgc
     5341 tagtgttgct cctatatttc ctgaaccaat aatacctatt ttcatgtttt ctgtttcact
     5401 atgttaacta tagctttgta aggatataaa gtgccttctt ctgaagcttt ttttaagact
     5461 gtaatccctg cgaaaccact acccaaaatt actatagtga actactcact gtgttcttag
     5521 aatctgcatg atttatcaat agtgggagcg atgctgcccc caattggaat atcaccgtta
     5581 tgcccgagtt gttcatgccg cctaccctgg ctgcatttaa tctacccgta aactttctgc
     5641 catttttgca cagtgagaag acatgtattg gttagctcct ccaaggcgat cgttccactt
     5701 tgccaccatc gtttgagcgc actggttggc tacgtcatca tcatgcgttt tgaacactaa
     5761 ccaaggatta ctaaatccac caacttgttc ggcgatcttg gtgaagttag caaacatctt
     5821 ggtagcatta attgcgctgc ttttgagatc tttaacgaac tccatctgcg taacaacgtc
     5881 ctgatgagta ccaagtctgg tcagatgacc agcaactaat gtatcgaaag gatagctcaa
     5941 ggcttgctca tgagctttga tgaagccagg cacatcttca gctattgcca agtccctgaa
     6001 aggaacccaa cctggaaaga cgacgtcgac caacataagg actttctggg ttggggcata
     6061 aatgaaggta ttacctcttt cgtggttgac acccttgtaa tctaatatca aggtctggtt
     6121 acctaacttt acagtatagg attttgaaaa tgtcagagta ggtgctggaa cgctcgtgtc
     6181 attgtgatcc ttcaagattg tagctgtctc ctgttgtgct atgtaagtag cgtttgcagg
     6241 aaacatacta gccgccccaa tatggtccat atgtgcgtgg ctatagataa caaccttcac
     6301 tggttccttt gttacctcag caatagcttt aagatagttt ttgccaattg tgaaaggtgc
     6361 atcaactacg atgactcctt ggttgcttac gaggaacatg gtattatacg atccatcggt
     6421 gacccaatac aagtggtcac gaatttcttg aaccagatag ccctttgcag gaatctgggg
     6481 tcccatggct atcgctggaa tatggattgt ggagtttaca gtattgttct gttttgctgc
     6541 ataagcttgt tttaccgcat aagaggcaaa tttcagatat ccataatctt cgtgatcctc
     6601 ttgaactagc gcttttgaat tttggatata cgaagtagat agaagggatg ttgctaagag
     6661 aataactaag aaaacactcc ccgattgcag tactttgctg tttagacttg atttattaag
     6721 catatttaca gtccccagat ctgatgatat aactcttatg gatacaaaaa catcacatgt
     6781 tatacaatct atatattaca attaattagt tatacacagc taataatgtt gctatggcaa
     6841 atgaaatttt aatacaactg aatttctgga tatttctaac cgagccagta cttgaagtac
     6901 aatttttatt tcctgctgca atgccatttt gccaagtttg actacactac cttcagaaaa
     6961 agctggattt gtttaatctc attactggga tacattgtat ctttgttagt tacttgtaca
     7021 atctgcttag ttgagtgagt gaggttatta ggaacagaca attcattcga actcaaaacc
     7081 agtatgaaca agttctacaa gaagagtatc gatttcatct tttatcatta aagttttgtc
     7141 caatgggatg tcctgctaat atctgtcaga aaaattttga acatcatcga tggaagtgtc
     7201 tattgttgtt attgacgagc tctaattgga cttcaaacaa tgatgcttta ctacaacgac
     7261 aagaaattta gctcaaaatc gtacgaaaag agtcagtcgg caagccaaag atgccttggg
     7321 tgctaaagat ctagtagaac aacaacagaa atctactagg tgtggtaact attactaagt
     7381 gagtagcaaa tactactaag tttaagtaac tacttgtagt aaaactaaga tctaatgaga
     7441 gaagatatgg tattgaataa aggtactaac gaaggggata ggcaaaaagt tgccattgtt
     7501 acgggtagct caagtggaat aggatatgca acttcactaa tgttagcaag aaaaggattt
     7561 tatacatatg cttcagtgcg taatatcaat aaatctgcaa gtctcgaatc gatagccaat
     7621 gctgaaaggc tacctttgaa attaattcag ctagacgtca cggacgatag ctcggtaaaa
     7681 gacgcagtag aaaagattgt tttagaaaaa ggaagaattg atgtgctagt taataatgct
     7741 ggatatggtc tattcggagc atttgaagat ctttcattgg atgagataaa agcacaattt
     7801 gaaaccaatt tctttggagt cataagagta acccaacatg tccttcctat catgaggact
     7861 ccg
//
LOCUS       DAOR01000007            3944 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_70037, whole genome shotgun sequence.
ACCESSION   DAOR01000007 DAOR01000000
VERSION     DAOR01000007.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_70037"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2835..2991
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1086 a          689 c          724 g         1288 t
ORIGIN      
        1 tagacgtgag aatgtaaagc tagcaatagt agtggtactg gcttcttgtg tttttccata
       61 tttgccattt gaaattgttg tgatttaggt actaatgcag gaacactgat agcatttggt
      121 agattgctct ctgttgtgat atttgtggtg ccagtgctga tgcctgtccc tgtgtttgtg
      181 ctgtccggat ccaacgtact gttctgagca taggctaaat gatgtaacgg gactgagttg
      241 agcatgatac taaggcgcag aacagtgttt aggctgacag atagaaacga ttctagaata
      301 acaggtagta atattatact cgtcgagagt aaaacaataa cgactatttt catgtggaat
      361 tatgaactac attatggata agagtcttct atatctaaaa ttttgctaca attcattcta
      421 tcaatttcta tggatccaaa ggagaataag cgttattatg atcatacttg tgccaattcg
      481 aactctaggt ctatttgtta aacgatacta agatggaata taaagggaaa taaggcgaaa
      541 ttatgctaaa aactaattgc gcttttattc tcatatttag ctattgagtg gccacaaatg
      601 aatattttca attcaaaaac aggtttgaca agaattgcaa gattgtcgtt tcgataaaca
      661 aataccatca aaattctatt attgtctaca tatcccgaac aatattacaa gtagtacatt
      721 acaagaccta caaactagtt attatttaga gttgcatttt gcatgttact tggtgttctt
      781 ttcatttcaa gatttcaaaa gactcgatca ttttttgtac tgtcggtaga taatatgagt
      841 atttttctgg ttctgcagta tagctaataa tatatgctct ctctcctttt actgtcaaga
      901 ccatcatcgc ctgaagatcg acgttcgctt ctagtccagt gtatacgatt ttgtatgcag
      961 gattgcctgc aagagttgca tttgactcac taaccctaaa gtcttgtctt aaatcactaa
     1021 ctgtgtcatt aacatactcc gctagcgttt taggtttaat atctgatata ttgtcaacct
     1081 gtacaattaa cttgcccacg atatctgagg tgtttttaaa cggagggctg aatgcaacaa
     1141 tgtcaataag tgtactattg tctttagaac tgctagtatt ctcttgttta tcccaattag
     1201 cgggatacag aatttttatt ccaaagatcg gatttacata cgtcaagaaa ttaatggagc
     1261 tgctgctatt tttgctctgt tctggagttt gcgccatagc ttgtcttgac tgaacgtaga
     1321 atgacatact gaaagcagca agtaaagata aggcacctat cgttattatt actgctggtg
     1381 aaaaaatttt gttgtacatc ttattgtaca gttgttaata gcttaatact atttgggttt
     1441 ttagaccgag cgaagtggtg cttatccata ttttcagata tttgcagtct cgatacgtaa
     1501 ctgtagtcat ttttagttat ataatgaatt acggttaagg caacaacaag ctattaaaaa
     1561 atttattaaa cttactatta gctattatat cgtctgagta aatacctagc gatattactt
     1621 ttcctttttg atagttcagt tcgtacgtcg caatcagagg gtctagtgct atacgttgtt
     1681 tatttgggac tgatgcatta taattcaaca gaattaggtc gttaggattt gtcaagtact
     1741 gctcctcgtg atgcctgtat tggaatggat tattggcaaa tgtgacattg catgaaaaac
     1801 aatagaagtt gctacctacc cattgtgaag tttcattcgc ccatctttct cttacactct
     1861 tccatgctga tttaccatta taagcccaac cgtgcccttt cactaatgtt attgtatgag
     1921 tgttcctgtc gtatttcacc tcagcataga atacgtttcc atccatgaga atcattgtac
     1981 ccccatctgc gacaaaccgt tttagattat catattcttg ttgtgtgaca tattcttgat
     2041 gtccaagtat cagaatgtca tacttgttac ttccatttac gaaaaaaata ccaccattat
     2101 ctacatcttc gtcagtcaac agatgagtgt tggatttagg aagagctgtc ctcatatggt
     2161 tcgttaggta aacgatcgtg gatgacgatg atgatctcgt caatagattg gttagtgggc
     2221 ttgagagcag atttaggtct gatgtaatat tctttcgtgc gtgcagcggt atacttgaat
     2281 ggacgaagta gaacttgtaa aatgacgcat ggtaggctgc agctgtaaac gtaggcttga
     2341 taagtgcaat acttaatccc ttagattcag cagtgaactt tgttgtgttt ttcaagagaa
     2401 gcaatggtct ggttgacatt attgctacta tcgtaattga aatgacgaat gctataatga
     2461 caatgctgag tacagatttt ctcatatgta ggattatctc gatcttgttt gattacattt
     2521 tagatattat ataccatgtt cttacgatgt actcatcagc tttgtgacga aacctgaaca
     2581 tgcaatccat tctgtggact cctcattatc ctacacagtt atcctcactc ttgccattag
     2641 aatttttctg ataattatgt tcattgtcgc cgatcataac aaaaatacat gtcagcacat
     2701 ctggtacgct ctaaaaatgt cttgctcatt cttttcccat atcagattgg cttgagcaaa
     2761 ttcaaatatc ctaagtcgtt ttttatatag aacatctgga ttacctttga aatactctag
     2821 ttgcggcact agcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natatagaac
     3001 atctggattg cttttgaaat accctagttg tggcactagc gtttcataac cctcgaatgt
     3061 cgagcaattg tctttcaggc tttcccgtat ggtcgtcaga gaactggtac acataacata
     3121 aagtccagcg tgggcatatt cataggcctt gttaggatta aggtacgcat gtgaccaatg
     3181 ttgcttgaat ggaaccaagc ctatggaatg attggacatt tccatgtaca tagaatctct
     3241 cttgagatat cccacgtacc taacattttc tgaagagtag ccttccgacc cgatgatagt
     3301 gaggtcacca atatcatggt tgccaaatag ttcaggaagt ccttctctat tgaggtgtgg
     3361 atatttttct ttattatgtg catctccccc agcataagta caagacaacc tcggatgagg
     3421 gagtggtttt gtaaagatac ctacttcggc tcgaagtgga taatttggaa ccacaaagat
     3481 tttgccggtg ttaccagctg tcgttttcat ctcctctgca attttagaag acacagttat
     3541 tgttgggtga gaggaaacga cttcactttc ccattttgtc cacaatttca cagcataatt
     3601 attaataaaa ttacgtctaa ttcttttcgg taaatcaaag actgattgaa cggcctctgt
     3661 cttttctgac gcaggtagat ttcctatctc ctttaataac cttgcctgaa tgggccagta
     3721 ttcatgatca tcatatacaa aagggatatc gaattccgag atcattttag ctgagaaaag
     3781 attatgtgca tggacaatat caggtcttgc ttcccggatt atttttgcaa gctgcttttt
     3841 cacagaacgc caatagaaag gaatgccgaa cctggcttta gcggtccaaa ccatgctaaa
     3901 tatttcagaa aaggtatcgt tattgtaatc ttttgggtat atac
//
LOCUS       DAOR01000008            3191 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_56945, whole genome shotgun sequence.
ACCESSION   DAOR01000008 DAOR01000000
VERSION     DAOR01000008.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_56945"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          982 a          584 c          605 g         1020 t
ORIGIN      
        1 acattcataa gaaagtaaag tacttgttca aggccagcag ctactagata agacctattt
       61 cttggtagct ttcggataaa catttcaaag acaccctttt cacttttgct gtcgctcgtt
      121 gctttatcag ctggtttaat gaggtagcga tagtaatagg cagcagccat tgtcaattcg
      181 taaaagtcag ttgctagtgc aatatctcct ccatctgcat ttcctgagat taattggcct
      241 tccaaaatac tgtcagctaa tcttcagtta gtaaccgtat aactttttcc tgaaaatctt
      301 aggtcacaat tacaagaagt gatcttgtaa taatgactcg aacgggtcat tatatagtct
      361 gccactttta attcatgaat ttgagtttca agtatcgtct ttagcgatag ttgcatcaag
      421 agaaccgact ctggcaagta tgaagagcta gctgaaataa gaacgaagaa acttgatagg
      481 atctgggttg acgttgatga ttttcaataa gtaataatga tgcgtttacg aagtattctc
      541 tgagccaata aaaagcttga ttatatttac aaactacttg cgcgacttta attgtcagac
      601 ggttctatag gttctatttt aatacctttt atccatctct ttgtttggct tcaatattta
      661 cgctcagttt attatctatt tctgttcact tcccaagcaa attaagaagg ctttttttag
      721 cgcacattcc acccgccatc cacaacctat cgctttggtc aaaacctgtt tttggggatg
      781 ggttaactca ggaacagtta acggcctcct taggaaatag gaaggctgat cttctcatta
      841 aaatgattcc ccctgtagtc acaacagaaa ccttacaaaa tggacaaaaa cctgttctgg
      901 aatacttagt cctggacaga cattcaagca tgcttttgat aaaccaggaa cagttggcta
      961 cttttgtagg cttcacccat tcatgttggg agaagtgata gcaaagtagt gaatgacgta
     1021 atgatacttt cacaaatgtt ctactatttc actgaaagtg catagggtat gcttaagaat
     1081 attattcttc aaattgttcc aagtcttttc cagtcgaaca attcatgaag aaggagcaga
     1141 aaagaagaac cactaagggt ttatcaggtg taccattaga ggaagaactt aaagagcttc
     1201 aaatgttatg ttcaaaatat gataccttta acatccgcaa ttgctagatc aaaaagtcga
     1261 gtcacaaatg ctaatagtaa ccagaataac aaatggtgtc cgcatcatga cgaattacta
     1321 ttacgttagg ttgtttaaaa ctcattcagt tattattgaa tagtgatttg aggaaaaata
     1381 aatgtcaata tctttagctg aagccttaag ttaccttaat acttttcgtg aatttattat
     1441 tgatagagat atgccagcgg ctattaaggg gaatgccatt tatttagccg ctttaggatt
     1501 gtgcacatat acagagatat taggcggtct ttattctgga gacctgagta aaatgcagaa
     1561 acaacactat acctctttca taaagcactt ctttcatcca gattatatga tagtcgatca
     1621 ggaccttaca aacgatcgtc ttaatggtct atacggagcg gtaagaagtg gcttggttca
     1681 cgaatatttt attaagaaaa tctctaaagt agagttagaa agtcccttgc ctatcaaatg
     1741 tggtattaca taaaaccccc aaaaaattcc tcaaattgtg ttctatgtta atcaatactt
     1801 tcaggatttt aaaaatgcct ttgaaaaata ctatagggat ttaaatactg accaatccgg
     1861 tgtcatggta gcaaagtttg gaaacgccct atgcagtata aattcaagtc tgattggtag
     1921 aatcgccaaa aattttcgcg aagatgtcag cggaaagagt atttaatttt cttatgggga
     1981 atagtgagag tattaacaat gatacggttg tggtatcggc tgaaaaagaa atttaattgt
     2041 aataatgtaa taatggcctt ttattcttaa gactataagt tctgctaact ttttctcgac
     2101 agattatata ttatatataa aaaacaaact ctcttgacgg actcagatga gtatctgttt
     2161 tccttcatct aactcctgta atcattcttc aactgcaaca tcagcggtga atcacattac
     2221 gtgaccgata ctaggggaca accccggcgg ttttattggg attatcaatt gtggtccttg
     2281 tccacttgcc tgtgatgatg gtcacacgta tatccaatgt agtaggtaga gtaagagaag
     2341 gaaacatgtt caacaagcta agagaaaaaa caagcagctt tgtggcaatg catccaaaag
     2401 tcatgatgta tggaattagt ctaggtgtga cactgggatt aacaataggc ttaagctatg
     2461 tctttgcacc acatgaggcg tttgctgctt gtataaggag aggtagttgg gcaagaccat
     2521 atctattcta gtaggtagaa tttctatcta accttctttt tttatttaca atatgtattt
     2581 acttactctt gacatcatca tccttagcga ctttagaata ttcctcggca ggccgaagat
     2641 gggtataatt acaactgtat tgtgaccggt acgaacctgg ctattcccat cccatcagtg
     2701 tatcaaatga ttggatgttc aaatgcttga aacacgacta atcagaaagg ggaattaacg
     2761 tgcctaatgt tattaacatt acaattctgc tagaaggtga agtcttaagc tgtctagttt
     2821 tacaaactaa ttaccattgt aaacaagatc tatatggtct tctcctgccc actccataaa
     2881 tatcaggtct tgctgcagtc gttttacatt aactgtgagc ttgccgcgct tccgtgcatg
     2941 atgtaatctc tctatttcca cttgcagtac atagatagta tcataaagcc tactgttctc
     3001 ggcccgggca tcaaatagca gctgctctac atcatgaacg ttagttttct gcaaagttaa
     3061 ttgccttgtt gtcatcaact tgatatagct tgttacttgt atacaacttg cgtagaaggc
     3121 tggcgtaccc tcccgggagg gcggcagcgt taattttgta tttttttatg tagcttgtag
     3181 cacaatagat a
//
LOCUS       DAOR01000009            5084 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_27841, whole genome shotgun sequence.
ACCESSION   DAOR01000009 DAOR01000000
VERSION     DAOR01000009.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_27841"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1575 a          982 c          945 g         1582 t
ORIGIN      
        1 atgtgatgat ttaatattat tttcaagttc tgactatgaa ggtgaatgat tcaatcatgc
       61 gggtcacgat tggcaaatat tgttgatatt cgtctacatc gcaattaaac aatattgaat
      121 atatcttatt gttgtgcata gcaataatat tcaagtgcgt cgaatatacg ttatttatta
      181 ggccattata cacgatcata tgcgcctttt ctcctccaag tgttgcctta ttcgtcgatt
      241 ctaaaaggtg gaaattcttg gttttttcta taataaatag cgtttcaatg ttggtatttg
      301 catagacatc taaagtcaaa tctttatcat tatccgaaat cgcaattcct agagctgcca
      361 acgaagtaag gggcaacgcc tttagaatca caacataatt tttgtttatg gcctcatggt
      421 cggtaatgac gataaaattc aatggtctat ttatttgaaa taagttttgt cctgtcttat
      481 cctggtactg atagcattgg attttctctt gttgtatcgg agctgtgttg tctattggtt
      541 tatcattctg tagttgctgc aatcttttct tttcagcctt aactaaggaa gctactctgt
      601 taggggcggc aatatttcta aatatgattt caggtttgcc accgtgcaaa aagactaaat
      661 cgccgacgtg cctagagttg gaattgctga aaccatggta cgaacaaaag tatctgcctg
      721 aagaaaaccc gctataaatt ccttgactaa cactacggtt attcattatc tgcacatcgt
      781 caagctctat caggggaatt tttattgcac cttcattatc tcggctcaag acaacgttat
      841 aatttgtaat ttcctgagtt tgaattacta ttttagtatg tttagtatgc agtttatgcc
      901 aaacgctttt tgtgatttca tttaaccata ggattacttc ttgatcatgc tgcccttgtt
      961 gttcttgcga catttttctt aatcctttgt gcctacgggt gttggagcca agttgtgata
     1021 tgacaccagg acatataaca tttttcgttt cgtgttacaa aacggggttg aaacggtgca
     1081 gaggttttta ttaatgatgc cgatccgaat tttacttcta accacaacca caacgaacga
     1141 aaaggaagta aaaaaaaatg gataatgtag agaattcttc tcttccatac ttttacatct
     1201 cactagccgt caaaaaacac accttaacca cattgtctat ctttttcgtt tcctgaaaca
     1261 gatgaaaatc attatccatc aacaataact tagtggtacg tattgcgcac cataaaacgc
     1321 tttagcttcc tctttattca gggcttaatc ctttaacaat tcattcttcc caactgctat
     1381 caaaatgaga gtcgtttaaa gaaatgaaac cttttcctcc ctactgtgca atgtaggagc
     1441 aacaggagtc aattcttgca ctctatgatg tatacgcagg aaattcatcc ccttttctgt
     1501 ggtcttgaat gttctttctc catccagata ttcgatcagg cctttttcta ttaaaagtac
     1561 caaatattcc ttagttactg tgtgcgaaag gtagccgtca tacattattt tcgttagtcg
     1621 tactctgttg ccattagcac tttcaagaat ctgcgcagta atttcgtttc tatcccggta
     1681 cttcatcaca tcgtaacatt agactaccca ataagaatgt cactatcata aagtgattac
     1741 gattatcaga ctttttttat taaccgaata tgcaattcgc gctgctagca tcggcccgat
     1801 ttaagaaaaa ttttaaggac gagaaaatat tgcattaaga attgtcctga acaaccagag
     1861 aaatcgtcac acgcgaggaa tctagtgaag ccgcgagcgt aggaaacatt gcaactcata
     1921 tttctaagcg gattctcaat atagaattga aggcatctag gcgccagctg gttcgttgaa
     1981 catcccctgt tatgaggtta agctgctgga ctaacttgag gcactcgtcg aaacaccagc
     2041 gaaacaactg agtgtttccc gcgggaatca gaacaccatg tcctccatag gaaggtgcct
     2101 aagcaatcag agccttaagg tcgtcattag tcaaaccaac agagtggtta tagaaagaca
     2161 tgcccgtagt gtaacctgta attctctctc catgtacaac tactttcgca gttccttact
     2221 tcgtactgtc tctaacttca ccatctctat catgaccggg tacagcttca aatatagcgc
     2281 cgcatgtctc accatcgaac tcggtcgcag ggcgcacaat tctaccaaga atatcatctc
     2341 gaattggttt actcagcatg gtagataagg tcgctctaat ttgaaaccac agactggcgt
     2401 agagcgttag cgagcggtta tggtaagcag tttggagcaa acgcacgccg actcagatac
     2461 cgaccaataa cactcacaag taggtaaaga tatcaaaaag tactgacacc atttacctgc
     2521 aaggtcaaaa cccgctggcc acatgtcaat cttgtcattt tattgggcac catcgattct
     2581 aatggatcgt atacattcaa taatgaccga caatagaaat attattcaaa tccctatcgt
     2641 ttgttcaaat aatcaacgcg tacagagata acaagaaaat attatgagaa gaattagaca
     2701 attttttgac tttattgagt atgatctaga ttattcttgt ctagaaaggc gctgtaatgt
     2761 ctgcaaataa aggaagaaaa gatatcaatt acaaagcagt aattgatgta attaaatgcc
     2821 ttgagagcag tgaggatagc gagtaataat gtctaatcta gatctaaggg ctcactttga
     2881 ctattaaagg aaacgacttt atagcatttt tacctatcat taaatgagca aacaaacaat
     2941 accgaaggaa agaaaatgaa aaataatgaa agtggattat atcaatagaa gggaatcgct
     3001 ctcaatccta aagacaacaa taccacttgc atttaagatg gtatttccaa atcgtgtcta
     3061 tattgccata gctgctgccg tattcaccat tttttggatt gcttttaatg tatttgatca
     3121 actgttgttc ttttcaccag tcgtaacttt ttatcttcct gatgacgcaa tagctggatt
     3181 catcctaaca aacataactt cagtattgat gggggtattg gttgccatga atgtatatgt
     3241 aattagaaat tcagagttaa agttagacaa atcattattc tcaggctcaa ttctaggtat
     3301 tgcttctagt acttgtgcta gctgttcttc aataggtttt cttgtcatat ctacatttgg
     3361 aggttttggc atcatcgcaa cagactttct tacaaattat cagatcccat taaggatagt
     3421 atcaataggt atactcctgt gggcattata ttcagtacat tataagatta caaagccgtg
     3481 catttttgat tcattcaagc aacagaaaca accgccaaca ccgtagcaaa tgtaacgaac
     3541 aggaatcatc taataatatt atgaaaaatg atagaataaa tgctctcgtg gtgagggtaa
     3601 ctattgtcac atttttcgca gcactagctc catctcttct cctttcatct ttgcagccac
     3661 accataatcc cttctatgct tttgctcaaa tacagaaaaa tgttgctcta tcattatcta
     3721 atctaataaa acatggagct tcctatcaag gaagtacctc agctcccgta actttaattg
     3781 attttagtga cctacaatgt catttatgcg atagatttgt aacagcgaca gagccgcaaa
     3841 taaattcaac ttatgtccaa ccaggtaaag tggcatttat ctttttacac ttacctaatc
     3901 gtggttttga ttcttttcct gcagcctcgg cagctcaatg cactaatgat caaggacagt
     3961 tttggcaata tcataactta ttgtataaca atcaaggacc tattgattct ggatgggcaa
     4021 atagagataa tttgaaaaaa tttgcatctc aaatacctgg cttggatatg cagaagttta
     4081 attcatgttt tgatagccaa aggcagaagc ccgttgtaga aagcagtgtt gcactggctc
     4141 attctcttgg atttacgcag acaccctcat ttataatagt taaaagtgat ggctcaaatc
     4201 ctcagaaagt agaaggaccg cagccgtttc cagaatttaa gtttttgata gataaagagc
     4261 ttggagggcc ataagaagga aggagatggt gtgagacaga agaagaactt ccaaatgggc
     4321 gcattttatt cgtccatctc acggtatatc ttatgggatt aatgccttga ttaaggctat
     4381 cagaaaacga cgacaataag aggaacgcag taagtattgt ctttgtgtat atacatgaag
     4441 ccatctcctg acttttaatg atcaagttct ttgataagaa ttcttctgat gcatctaggc
     4501 caaggaaata aattgtaagg gtaccagaag cggtaggaat actggcgtta ggtttgcggg
     4561 gcacattgaa gtattacaat aatacaatga tgatgaagta gatgtacata tcaagtccag
     4621 actagaccgc cgcttagtta tgtccccagt cccacggaaa tgggctagta ccttacatac
     4681 ggactcatcc gcgcaccaag aaacgtctca cttatatggt cgactttttc ttatcatctt
     4741 atgaaaacta taaactgtcg tgaagcagga tttgactgcg actacgtcgt caaaggcgaa
     4801 acagaagaag aagttatgaa aaatggggga gaacatgcga tgaaagtaca tggtatgaaa
     4861 gaggaagata taactccaga aatgaaacag aaaatacgag gacttattcg cagttcttaa
     4921 cttttttttg attctacgaa ataatcttca agatcagata ttacttcagg ttgtatttga
     4981 tttgaaaaaa tagtatcctc aagtgccatt tgggctgaat cacactcatt ctaaggcctt
     5041 cgaggcaata gacatggatt gatttagctt gtagcacaat agat
//
LOCUS       DAOR01000010           21968 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17148, whole genome shotgun sequence.
ACCESSION   DAOR01000010 DAOR01000000
VERSION     DAOR01000010.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 21968)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21968)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21968
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17148"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    17742..17958
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6630 a         4405 c         4241 g         6475 t
ORIGIN      
        1 tcatgatagc ttaattcatt atgagaaatg tattcacaat tatgctgatc tgagtcgtga
       61 taccaatcct tatcgaatct agaacgaaat ttagcagagc aagtcatgaa ttttctgcaa
      121 ggccaatcca tggcgacttt cctaacgatc tccttttatg gtccgaggca ggcatacttc
      181 atcaaaaacg aaagttactc aggcctcttt ctgccaagta atcttgctac ctacctggaa
      241 gtgaattaag gtctacaatg gctatgttcc tacctctttt agtatagaat caaggtctga
      301 ttcgtttaga cctgcgctca tcttagcatc tctccttgat atgatgttac ctttgtcaaa
      361 ggcaataata gtaggaacag catttataga aaatctatcc catagtggat tatcatcatc
      421 attcactttt gctccataca ttttgtaact agcagtagta gatttagatt ttgcagcagc
      481 agattcaaaa aggggtttga atctttgaca aaaaggacac caatctgcgt aaaacattac
      541 taaatccttt ccgcctatgg cgaataattt atcgaattga gttggttcaa gatgttccat
      601 aatatcatat aacatctaga tgaatatatt aattaattca cgctgcggga cagttttctg
      661 tttatggttt taccaattcc agttggtatt tggcatttag ctggcctcag gatatttggt
      721 ggttcgtgaa atgatagtct taatgaattc agctaggaca taattttctg ttcattgttt
      781 tactataata gattgccttg gcagggccta caccagattt tttcctttga cgagcatata
      841 cacgtttgcc attaaatata aaagatagtt gattcttact tctaattaac tgccgcaaac
      901 gatgttttgg ctgtttcgcc agttccttac tgatgtttat taatgtacca ctatcgtgga
      961 atacaatatc gacccaatgg gtaacattgc aaccaaatgg tgggcttgga ggtgaagaaa
     1021 cataaactgc tatatagcaa tcaatttcct taatcttctt cttgtattat gatagacaac
     1081 gattcggtca aggagcaagt gcgtgattga agtgataaaa ataatacgag atcccgacgt
     1141 gacttctttt tgttttgtga aatacagaag ccgtatcgga attataagaa tagttcatgc
     1201 taacacaaaa taaaagtttc agccagtttt cgttgctaaa ttactcataa tagattaatt
     1261 tacgctgctt atggattatt gttccgatca aagaacctgc acattctaat tcttaacgaa
     1321 tagacgtcct cggtgaaacg gtgcggttca ctcaattttt tcatgtttca acattgaaaa
     1381 ttctttcgtc ttaagagcat aaaattctta agagttagta ataatcaaaa caaaataata
     1441 aattagcatt tgaagagcat gataaaataa ttcctaactt aatattcata aaggattgct
     1501 gttaagcttg acttctaagt caatctctgt actagcaggg ttatgaaaac aattgcatgc
     1561 agagaagccg gatttaatcg cgctactgtt gtcagaggag agacagaagt tgaggttcta
     1621 ggagagggac gcgagcgtgc catgcaagac cataagatga cagcaaagca aatactccag
     1681 aattcaacca gaaaatcaaa gggcttattt gaagtcctag tttttcacac cacgttcgga
     1741 taaagcagaa cacgactgct atcaatgatg gtaacaatag caactagacg ttttgttctg
     1801 catttcttcg ctttgacgac tattcttctt ttcttcgctt tgaggaacta aaaggggagc
     1861 ggccaaggac gaggcccttc tcgttcacaa gtcctgaaat ccggaatata tatatactaa
     1921 taattagtat actatttaat tggtggctag acattatcca aagttatgat ttggaaaata
     1981 gcataggttt tttgttatat aaaactgcaa tggcttttcg taaagccctt gatttagagc
     2041 tgcgtagaaa gacgggagtg acttttggtc aatggaagat tcttaccatg ctttctagag
     2101 aagatggact gactcaaaaa gaaatagctg atagatgtga agtggaaggt cctactctta
     2161 ttcctattat cgataaaatg gaaaaggaag ggttcgttac acggaaggta gatcgtgaag
     2221 ataggcgaat aaacagaata tctcttacta ccagaactga tgctgcatgg aattcattgg
     2281 ttgattgttc aatgcaagtt agaaaatctt caatacgaga tattcctgaa gagcaagtca
     2341 agattatgcg aggtgtcctg gaaaagatat cgcagaacct aatgactgac tttggagttg
     2401 atcattattg taaccccagt tcagtaacat cgagtgctac tgctagcact gaccaacgaa
     2461 gaaaaaacgc tagatataag tgaggtccta aatagtggat tcgcaaccaa atcaaaataa
     2521 atctaataac aatacagctg gacaatcacc gtcatcgccg aattattctg taaaaatccc
     2581 agcttctgca tggaagatac ttgtgatcct tagctgtctt gctacgatgg tcatgtacgc
     2641 agaaactatg cttataccag ccatccctga tcttatcaaa gactttcatg tctcttatag
     2701 tatgtcatct tggatactga ctgcatacct aatcacaggg gctgtaatga ctccaattgc
     2761 aggcaagtta tctgatattt acgggaaaaa gaggatattg cttattatta tgataatcta
     2821 tgcaataggg gtttcaattg cagggtttgc tactaatatt tactttctgg tgtttgctag
     2881 agccattcaa gggattggga tggctatgtt tccaattgca tttagtatta taagagataa
     2941 ctttccaaga gagaaattat cgattggcca aggagtaatc agttctatgt ttgcatctgg
     3001 ggccgtgata ggtctatcag tcggaggagc aataatcaag cactacggat ggcaggccac
     3061 attttttacc ataatcccaa ttgcaatagg tctattaatt gttgtatcac gctttattcg
     3121 tgtaaatgaa gaactagagg tagaaaaaga aagacagcca caacaacaag gtctgtttgt
     3181 agaaacggag gagaaaagat ttgaatccaa agacaataac gagactaaca acaacagagg
     3241 tagtaaaggc aaacgccgca gcgtcgttga cattaaaggc gcaataacat tggctgctgc
     3301 tgtcacttca tttctactag tacttaccta cttagaaaca ggtgatagtg ggggaacatc
     3361 aacaggttcg ctacagactg tcatctttct tgttataggg attatctcgc tagcactctt
     3421 tatattagta gaaaagcgat caacaaatcc attagttgat tttagattac tattgcataa
     3481 aactgtcctg ccagcaaatt tgataataat ggttgtagga ttttctatgt ttatggtatt
     3541 tcagacaata ccaattctgg taaggaatcc accaccactt ggatttggtg aagatgctgt
     3601 tagtactggt aacatacaat tgccatttgc ggttatactc ttggtatttg gaccgacatc
     3661 tggttttatt atatcgaagc taggatcaat gaaaccagtc atagcaggtg catctattac
     3721 ctcagctgga ttcttcggcc tccttatgtt ccattcaacc accttatcat tatcagcaaa
     3781 ccttgcaatt ctatctactg gtctatcact aacaaatgta ggagcaatga atgtgaccat
     3841 actatctact ccaaggcagt tcatgggaat atcacttggt acgagcatgc ttatgagaat
     3901 tgtaggagcg gcagttggtc ccgcacttgc aggaatgtat atgcagtcta atcaatctat
     3961 actgaggata aacggaatag ctcaatactt cccctctgcc gaatcataca acctcatatt
     4021 cctcactgct acggcactct caattctatc cattgcattg gctataatat taagacgcag
     4081 ggctattaaa ttggcaatac caaacgtccg ctagtataga agaatagcta cacatcatga
     4141 aaaaagccgt tcatcgaaga gacttgtata tcgtaattct tcgagcgtta tgagtaaatt
     4201 cattgattac tacagctatc tgaattttta atcaaattcc atgctgcatg atttgctgct
     4261 cctaaaatgc ttttaaattg gaggaactat cgtatgaagg cttctgcgca atgactgctt
     4321 gaagcagtat tgtaatatct acaagtgcgc gtcgggaaca agttactcaa ctcaacctcg
     4381 caatagtcta tgctgctccc aattcctatt aacaacaata ccttgcatgt gcaacgtcag
     4441 cgacctcatc tgaagatttg gattttacaa agttactgcg acctagtact acgtattatt
     4501 ggaatacaga ctagaagtta gttactggac tacttttagt ggggtgtaat tattccagga
     4561 gttgaggtat ttgtagcagc aggagcgcga ttaccagtat tcacacctgc tccagttacg
     4621 ttcacactat aatacgaaac tagattcgcg ttgttaccac aggagaattt ggcagttatt
     4681 ttgtttgttc cctctttaac aattgcgtag gctggaggaa gtgtaaattt ccattttgaa
     4741 taatcacttt gtcctctagg acctactcca gtagtattct ggtatggctt taccccattt
     4801 aggataacag agacatgaca attagaatta ttagtagtac cgccagtagg tgttgatata
     4861 ccagaaacta cgagattctt tccaacagga acttgttgac cctttgcagg tgacgttatt
     4921 ctcacagcat gtatctttgt tgttacattg gaagaagatg tcgtcgctgc tgaaactttc
     4981 gtgttattag cagtcgtagc ggtagaagaa gataaggcct tggcataagc gactgtatcg
     5041 gcattcatac cgacagtatt atacagacca actattacga aaacaaacgc cagcagtatc
     5101 agtctttcaa accctaatcg agtagcctgt ttcatgagaa cttgtctccc acatcattca
     5161 taaaaaggac tttatcaaat atttcagctt cttgactttg taatattata ctacctattt
     5221 acttattaat actgctagtt tgttcctcaa ttatacacgg gctaggcttt ttgtcgtatt
     5281 agaataaaat ttctcatcac tcgcatttca gccaatgtct caataaccga ggcttgggtc
     5341 aaaaggccca aaaaaataat gcttaaaata gtcctagata accattgttc ttatctggca
     5401 caacatgggt aaattcgaac tttattctaa taatgcatca ttatccttcg aaagtcatct
     5461 gaaaggatta cgggaacaac cgaagacttc attgcttgag ataagaaatt tcgtaaaatc
     5521 tcttggtgat aacgtcatag aagaagttag gccgcacaga attgtttacg ctaagtctct
     5581 cacctttaga acattcttgg atatacaacc aaaaaatgac tctctgatta tttctacaag
     5641 aactagtaga aatgagtcag ctacaattct gactgttagg accatgcaag aagtggaaga
     5701 tctcaaatcg caaatcaaaa atgcatatca gactattcaa taaaaccaat aaaatccgcc
     5761 ggggacaaat caataaaatc actaagccat catcgttcaa cagcctcgtg actgtaggat
     5821 agtggagtag tttggggcca ccttaattga gttcaagaat tcataattgc atattatttg
     5881 aattacaagc aaaggtcatt agactgcaat tcgacgagat tggaatcatt gaagtgaatg
     5941 ctatgatatt catgaactga aagcacgcag gggagttcat gggctttcaa tccacatggc
     6001 gaacacgatg attactgtca gttaaggagg atttattgtt tgcaaaatca agtcaaaatt
     6061 gtccacatga gacaactgaa actctacaac agacgtatct acgacggtct gcacaaatca
     6121 tgttaaagct gcccaagatt taaacgacga gcttatatga aatcaattaa cgaaagcata
     6181 tgagacaagt atcaacaaaa tataacacat cttcctttca atattgtcaa cacagtattt
     6241 ccgattttga tgagacggca aagaatagat ttctatacct tggttttatg tcttgccgtt
     6301 ttaactatta ttttatcatt gtcatctacc gataactacc tcattccaaa agcagtagca
     6361 caaaaagata actacggtaa tagaacaggc aaggcatcat cagcaacacg atttaatcaa
     6421 tctgctatag agtctgtaaa tacgtccctt acgagtccta aaacatcggc ccacaactct
     6481 tccaaattga atgaaggcac tgcgactgtg gcatcaccac ctaatccgac taataccgtt
     6541 gaatcagtta tcggatcttt tcttaatccg aaagttctat tgtcctctgc tatagatcaa
     6601 attcgtaata gtaccgccag tagcgttagc agcaatgact ctaatagaaa cagcacgatc
     6661 gttggtggcg actacgatgc ggtgttatta tcacgtaaaa taattgcacc taaggatttc
     6721 atacctcctt ttgatgcccc accttacaac gaaatgaaag gccacgtgtc tgcaaagctt
     6781 ccatctaacg cgaactcaac atcgccattg aaaatattta ttgcgaaaat cgtggttggg
     6841 caaacactag agctcaaacc agttgaacta caactcgtta gtgatttatc taaacctggg
     6901 tacacatgtg tgtatcatga gagccttccg ttcacaactg ctactagaac tgcaggtgcc
     6961 agtaatgaag ccaaaggaaa taatactaat acggcgctat caggagcaac agacaaccta
     7021 acaattaccg acattggact tcttaatccc acagacttac caagtatact tcccgatact
     7081 agtagctgtc ggattcaatg agatgatacc ctcggttctt cgccatccat ttaacaagta
     7141 attattatgg attaaaatct agcttaggct aatctgatta cgctataaat tccaactata
     7201 ctttcttaaa ggttttacct tgagaaatgc ggcttcgcct ttcaacatat ccatgtgctc
     7261 tacaaccttt tttgtcaaaa tgaccgacat atatcagtac tttcgtagaa gggatcttgg
     7321 ctgttctacg gcttaacaag ccctcaagat ttgcttttat ccgtcctacc aatatctctg
     7381 tctcgatagg tgtaaactcg gaaaactaca ccataataaa gtacccatga accactatta
     7441 cagatggtct gatattatct ggacgttgaa acccgttcct gcaaaatcta atcagataat
     7501 ataaaagcat agatattgtt atttcgactg aacagagatc tcgatgcaaa aaagtgaaga
     7561 tgttgaacta cgatccagtc cacatccaca ccgcttggtg acctacgtaa ttgttatcca
     7621 ttcgtaaaat gggctggagg taaaactcag ttattagttg aattagataa agtaattccg
     7681 tctgaatttg gccaatattt tgaacctttt ctaggcgggg gtgccatgtt ctttcatttg
     7741 atttcgaaca gaaagaggat attcaatgcc tatcttgctg acttaaatag agagttaatc
     7801 acaagctata aaattgtaaa acataatgta aaagaactaa tccaactcct taaaaaatat
     7861 gaagccgaat acaagaaaga ccctagcgag cgttactaca cgctaagaga tacaattaga
     7921 cccaaaaatg atattgaaaa agctgccagg ttcatcgcgc tcaacaaaac gtgctacaac
     7981 gggctttatc gagtgaacag ggctgggata tttaatgtgc cgatcggaag gtacaagaat
     8041 cctccgattt gcgatagcaa taatctagag aacgtcagtc aggctctccg ttattccaag
     8101 gcaattatac aggcgaggga ttatagacaa atgatactgg aaagggcaga gaaaggcgat
     8161 tttgttttcc tagacccacc gtataatcct acaagttcta cctcaaactt tacaaactat
     8221 acacattatg gatttggtga tgatgaccaa gagcaacttg ctagcatctt tcgaaaatta
     8281 gatgatagaa aatgcagggt tcttctaacc aattcagata ccccatttat tagagagtta
     8341 tattctgatt ttgcgacaaa tattaaagaa gtaagtgtat taagggctat caactgtaaa
     8401 gcctctaaac gcaccggtca taaagaatta ctggtacgta actatgataa cgatccatgt
     8461 ttttaaccac caatcagcac atcctcaata ttggcgatca agcttgaccc caatgacgac
     8521 aagaacaaca accaaacctt cttagatttt agcacctcag acagtctgac cgtaaaacaa
     8581 tcctttattc taaacgatga tcgaacttgc aacagaagga ttagaacctt cttatttcaa
     8641 caagtcaaag aagtcaatat gccatactaa caccctgact accgcaaatt acatcctaga
     8701 tataaaaaga gagatcaacc cttcacatag gattaatcta agagcactcc taaagctatc
     8761 acaattcttt ggtgacaaga tttcatttag aaaaatgttg aggaaagaga tttttacttt
     8821 cttggatagt tatcgcaatg ctgatgcgat cggctgtaca aatggattgg cacagtatac
     8881 ctagttatac ttgtaggatt cattaaatgg tgatgttttc cacaattggc gtctgttgat
     8941 aggaagaaac caactgcaac aagaccgtta atgactttcg atttgctgta taagccggct
     9001 taactgcgtg cgggaacgaa ggttaggaag gataatgact gcgatacgct agaaacatct
     9061 gggatatcgg agaacaaaat gaaagggtta atcgctatat tgtatttatt atgtgaaaag
     9121 tagaggtaaa ttatcagcgc caccttagtt cctgtagggc gctgtttgtt gtattctcta
     9181 atcagcctgc tgctcttaaa cctaccaaca ctatacacat gatgctacaa tgtgatattt
     9241 gcacggtgtt ggtatttgct aacggaaact tgtcaaaaca aagcctatct agtttaccaa
     9301 acggttctag tgaggacaat aacagtaatc ttatatatcg acagtatcaa cctacctaca
     9361 gaatatcgaa ttattcctgc atggaatggt tccgcgcacg tattacgata ttataaaaat
     9421 ccggtctacg ggaatcattt actctccctc ttttacaaca tttagcaata acacctgact
     9481 aaccgtagta gtcatctgca aagttttaaa aataataaat ttctatgcgg caccgtatag
     9541 gcacactttc gacaagaatt tttccttgta ataagattgc ggaatagcgg ccagaacatg
     9601 tcagacatgg ttagtatcaa tccattctcc tgaccttctc ggtaatcaat aacgatccag
     9661 tcgctaatgc caaccgtttg ctatccttta caggaggcgg cgcctccttt tacagggtta
     9721 aaactactcc aaaattcata ccccatatcc aaccatgatt gacaggatca gcagacgctt
     9781 agaaaataag aagaacatgc tacactctta gaaatacagc ttctccagaa cattttctga
     9841 gcaggtgatc ctgataatgc atccgtcgca tatctcgata caggagtttt gaatcgagct
     9901 atcgatcgtg gcgctttttt ccacgctcac tcgttcaaac aatctcttat atgaacagat
     9961 aaaagatcaa acgtctaaac gcagaataac atttacattt taaaaggact aaaaaacaag
    10021 ggatattatt tagcacggaa agcaataata atactgctag aagcaacatg aagattgcga
    10081 ttgtaacggg atccggtaag ggaataggga gagccatagc tttgacgttt gcaaagtcaa
    10141 agggatattc cgggatagtt ataaacggta gaaaaatgga tgaagtacga gaagtggctg
    10201 atgagataag gggtgaaggt tgtgactcgc ttgcaattga agccgatatc tcaaatgaga
    10261 atgattgttt tagattagtt gaagaagcta acaaagcata tggcaggatc gatgtattag
    10321 taaataacgc tggaattcaa actgacgtcc catttgagga cacaaccacg gaagaatggt
    10381 acaagataat aggagttgac cttacagggc catttgtatg tagcagggaa gtagcgaacc
    10441 atatggaaaa acaacgtccg aaagggggct gtattattaa catatcatca gttcatcaga
    10501 caataccgaa gccacactac aatccttatg ctacctcaaa ggcaggtgtc gaaatgatga
    10561 caaaaactat ggccttagaa ttggccaaag acaatataag agtaaatttg gtggccccag
    10621 gtgccatact aactgatatg aatattgagc ttaaagaaaa taaggcggag gtagaaaaga
    10681 tactaaagca aatacctatt ggaacgattg gaactcctga ggaggtagca aatgtagtcg
    10741 aattcctagc atcagataaa gcaagttatg ttaccggtgc gtcctttttt gttgatgggg
    10801 gtatgaccct ctatcccaat ttcggtatag acccagaaca tgatgcacaa agacattata
    10861 aggagtaata atggattact tggcctaatg tagacattag tctaggctaa ttttcttaga
    10921 ttattcttaa gttaagtacc tttattggta gtattagtgt ggtttacgta taatttgata
    10981 aacagatgaa atattttggt ggccgtatag cacttaccgc ttattgatta ttgattttat
    11041 ttgattgcag attcgttcga atttgcgctc tattacttat tgtgtagttc ttccaagacc
    11101 tcatctaacg acgtatctgc gaccaagttt cgtgccaagt gcctcttata aaagacgcac
    11161 agagtgcttt tagcattggc agatagtaac gtgtatcgat tccataattt ttcagctgaa
    11221 attctgtctg gggtcgaaga aacaacatat ggtggtacag ccctactatc taggacgtgg
    11281 cagttgttgc tggagaactc aaatatagcg aactagtagc tatctcgtat tattccccta
    11341 taatctgaaa tacaaccttt cgttctcgat ggcgagtatc catttctacc aagactattt
    11401 gttgccatgt accaagtatc agactgccat ctttgaaggg aaccgtcaaa cttggtccaa
    11461 tcaaagatgc acggacgtga gagtgtccgt ttccgtcctg ccaaattttt tcatgttcgt
    11521 aatagatctc tttcggtgcg actctggaga gcatctccgg aaaatcctta gcgagcccgc
    11581 gttcaaactc tatagtggta atggccgccg tcgaccctac aacaaaaaca gttacaatgc
    11641 catctgcgag tttactatca agaactacct ttgctgtctg tttggtcaaa tctatgatat
    11701 cgttctcacc tttagactta atctggagaa ctttaccgag aactgtcatt atggcataat
    11761 gagagacggt aaaatatatg cgatgcgcga tttaaaaact cccaattcac atcagctgat
    11821 ccctgcgcac caacaatgct ttagttacat acaaattcct acatgaatta aaattggtta
    11881 gtttgaaggg aaaggtagta atgcatcggt atgaaagcaa ggtgctcaaa tccaacccgc
    11941 taaaggatcc ttatgttcgg gagctcgtga tctatcttcc tccaggatat cggcaaagcg
    12001 attcggctgg atacgtgaca gtctatggac tcgtgggctt tggcggacag gggaagatga
    12061 tgctcaatgt tgaccctttc actgagacca ttgaagatcg gatgaaccgt ttgatttctc
    12121 atgggaaatg tggcccaatg atacttgtgt tgatggactg cttcacaaga ttcgggggaa
    12181 atcagtatat caattcttcg gcaactggaa ggtacgagga ctatatcatc aaagaaattg
    12241 taccatttgt tgacaaaagc tacaatactg ctgctggtgc acgtgcgcta tggggaaagt
    12301 cgtcgggtgg ttatggatcc atcgttttag gaatgcgtca ccccgaaatt tttcacggac
    12361 tagcagatca ctccggcgac tcggcgtttg agtattgcta cctccccgac tttcccaaag
    12421 caatggaggc ttttagaaaa gttggtagtc cgggaaagtg gctggctaat ttctggaaga
    12481 aaccaaaccg tcatcaagca aacgacgcac cgcctttgaa catcctagcc atggcagcgc
    12541 attactctcc aaaccgtcga tcaaaagaga tgggggtaga attgcccttt gacctcaata
    12601 ccggtgagat ctcgcaaaga gtatggaaga ggtggctttc ttttgaccca gttcgaatgg
    12661 ttgaaaagta tcgcaagaac ctgagcaaat taaagctaat ttatatcgat tgcggcacaa
    12721 aggatgagtt caatttgcag tggggcgcaa ggatacttca ttcaaagctt gaaaggatca
    12781 acattgacca ttattatgag gagttcgaag atgggcatat gaatataagc tatcgctacg
    12841 atgtctcact tccaaaaatc tatttggcgt tgtcgtagga atctgaacac ggtgtattat
    12901 agcacatgta caacaaatta gaacacacct atttttgtta ttgaataagt gatggctttg
    12961 acatattaca gacgaggcta gctcaaaccg acgaatatcc aagcaagcaa atcttacaga
    13021 agatgtcggt ctctactcca agcaagttat agtgctaact tctgcgaaat aaataagctg
    13081 gcactacgta tgtgatactg ctctcccctc gtccgaattt tcttttgagt cgatctcgac
    13141 ttctgtcttt tttctgcttc gatattatct tttttaattt cttctagctc atgtttggat
    13201 tctgtatccc gtttttgttt ttttggtgat gacaactctc ttcgaacttc gatatccaaa
    13261 ctactactat tattattatg atactgtggt caactaaata attgggcaac aaattgacga
    13321 tctgattcac tacgcttctg tattccttct atgtgaagca caagcaagag aataccaata
    13381 tatattgcat attatgtttt tttaagaaca tgtcacgtcg cctgtaaagg ttcctttttc
    13441 gccgttgtca gcatttaatt ctatagttac tccttgccca cattgcccgc taatcgtagc
    13501 aggtgttggt ggattgcctc tgcagatgct atcggaaatt tgacttccag taagagtgta
    13561 ttgatcggat ccaatatgtc ccgtctgaaa agatcccccc gtgttctgcg atgcaccaga
    13621 agtgcgaata ctaacaactt cccaacttcc gtgtaggggg acattgctct tgaaagcaac
    13681 aaacgatatg cttccaccct caactctcgt accatccgag caagcaattg aaccgctccc
    13741 gctgcctgat actccctggt tattattaac tggcaatcct cttagtgcac cattggaaga
    13801 agggattggt gacgaaggta atgatggcga aatctcatta ttgattaata ctccaaaatt
    13861 cccccgaaat aagtcggcac tggcataaga ataccatgaa gactccatga gtagtccgca
    13921 cagtgcaacg acggaaacaa ctccgatctt gggtgcaatc cctttataca ttccgactct
    13981 ttattctgcg cttccccatc agttatacat tatgtagttg cttactagtt tgaccctgta
    14041 tagttatgta ttaggataat ttacatggag gaattatcgt agttatgaca tgcttatgcc
    14101 atctgttcgt cattacactt cttacatctt gcaagaattg catccgcaga gataggcttg
    14161 aagagccact agcacgagat ggattcgtag atttgagggt agaatcaaat aatggccaaa
    14221 acctgcctcg tcatattgcg actagatctc tcttgggagg tggttccgga tttccaaatt
    14281 cattcgaata tgcggagtac aaccttattt cttgcccatg tttaatatta taagaaccga
    14341 tagattttat gatcttttta tactcacctc tcaatggtat caatttctcc gctaatacat
    14401 ccgctatcaa tatgtgccaa ttatcgccca tctccataac tcgacgagct aatattatgc
    14461 cgggtcccca aaagttttga ttgttcttaa tatcattcac cgcaaacacg ggacctgaac
    14521 tcaagcctat cctaacacca atgaaatcat ccggtgattc tttgctcctg ttatagagcc
    14581 gaagctttcg atgaagctgt gtcccaagtt ctaatggaag ttgtggattt aaaagaaatc
    14641 caatcaccat gccgtcgcct gtgggcaaat ggattttcat atcgtctgat ttaaaagcgt
    14701 cacaagattt gactatacca aataggttct cgattttttc tcgctgttta gttacagata
    14761 ataagggatt agacaacccc actatatcta tatagaaaaa atttgcatca acgacttctg
    14821 atgatatccc catctcagat atattcaaac cgtagactcc tgcaagcttc ttgtatgttt
    14881 ccacatgata atcatgaaaa ggaaagccgg catgcattat agggacatcc aaaataggtt
    14941 tttggcacaa ataacaatgc aacgaagcag atcgatcggc gaaaatcagg cttccatcat
    15001 tgctaatcgt gaaatcgatc cacgaagggt tatgatgtag cccttttatg gaaagcatcc
    15061 tgatattcct agtcaataca ttatctcctt caagcttcat ctcaagaatc ccgtctacta
    15121 tggaacttaa gattacatac tcgtttgtag aaggcgtcgt caaggtaaag atggcctgtc
    15181 cttcaatctt cttcaaaagg aaataaagtt gggtcacgaa agttattaca gaactttctc
    15241 cgaaaatcgc aaataaatgt gtcaaggaat caataatcaa gagaattgct ctgttggtgt
    15301 cgttattggt actgactgca tcagaattat tcataccatt tgttgtcgtt ttgccgttgg
    15361 tcattatact ctctgtaata tcattgatct cttctaatgc taggcctaat ttctcgttgt
    15421 cttgcccctc gattaggtat gggtttataa atacggaatg ttcaggcaga tcttttctgc
    15481 tggcgaatag acttttgtat tgattttctg ataaagttga gctaatatag atgcaatgat
    15541 cccctttatc taaaccgtca ataaagaatt gtcgacagta catggtctta cccgccccca
    15601 caggaccgat tagaagtaac acgctgttag gaagaccacc tccttctacc aagtgtgata
    15661 tgccaggaat ccgcattgag ttgaaagtgt aaatctatta gtaaatactt ataggttgtt
    15721 gtcatcctaa gctttcctaa gaaactaact cacatcagtt ccaaattgtc aaggtcgaat
    15781 ttctttagaa catctttgtc atggttcagc agtgatgcga atattccata ctaccttcgt
    15841 ctgtactttg cgctgtcggc cgagttactg tgcgtatata ataacactct tcggcgaatg
    15901 aacttacgaa gttaagcttg aatggtctac agagttggct acacaccaaa taggattctt
    15961 tgaaaataaa acatgcgcta aaaatctagg agggctgata aaaatattaa aaacccggta
    16021 gtgagtcccc cactcccggt cgttcttttt cgagcctagc aatatttcga gacatgtatt
    16081 ccatcgtgcc tgggatgtga cacttgcaag cacaatcact acagtctaaa tgagtgcttt
    16141 tcttacaaca ttcagataat tcgtcgttca tcgcaaaaat acgaaaaacc tttacaaaag
    16201 agtaatttta gtcttgctac ggattatcag acactcatca gatacctctt tagctaattt
    16261 ccttcgtaag caattaccag cttattcgtg aaaccaggtg tgtgtagcag atttactcta
    16321 tcgaccaaga atccagaggt tttccaaaat aaatggagtt ctttttttcc tacaagctgc
    16381 cgaaggcccc tcccctaagc ggaagaatct attataatag ctcgcactag catatagatt
    16441 tttaacttcg ttgaatcgat agattgtggg gttttcgact taggcaaaag gaaagtattg
    16501 agtgaatctc atctcaaaga attggttaag ccacagcaag gcgagttgct tggtcgggtt
    16561 atcaagcttg ttggggggga caatattatt gtaagatgca cagatggcaa ggttagaact
    16621 tgccgtattc gagggaaaat taaacgtaga atgtggattc gagataatga cttagtatta
    16681 attgctccgt gggactttca agcggagaag gccgatattg tatggcgata tattgccgca
    16741 cacgcagaga agctggagca ggatggatat ctagaaggcc taaagtaggg ctcccgcctg
    16801 cccaacgggg gggatacacc ggcgcaatat gcagttgtag cagaaccgtt ggacgtcata
    16861 aacacatatc tatccttttc agtagcattt gcctgaccta aaatggacag tagcatttat
    16921 cgctaccgag aatacattcg gttgctcgaa tatctaacac aactacgaat tcgatttatt
    16981 tattctatat tttatttaat taataagtca ttacggctga acaaagtgtt ttgaatttcg
    17041 attaatgagt ggtattattt tattcattat aatctcatga aaattagctg tctcaacgtc
    17101 gaatgtaaac ataattacat aggcaagacg gttattttta tctgttagag ggatcgtggc
    17161 tcgaagtaac ttatcataat gcgagcatgt gtaatctatt ttgcccaaca gagtctgcca
    17221 tcttattcta gtatattggc gtgtcgccgc agttattgca tattgctgtg tttcctccaa
    17281 gtttaataat ggcttcagat ctttgcgctc agacatcgaa agcaaaagtc catgttcatc
    17341 agcaactccc acgaagcgaa ttgatggatc taaactaata acgtcgttac aaaattgttg
    17401 aaagaactgc ttcattctga cgaagtaacg tcaaaaagac ttacttttat acttttgaat
    17461 ggttgtacta cgatggcaat aacaaccaat ggatctagcg ccacgtacgg ctacttgacc
    17521 aatgccctac aagtcacgaa tatcgagcgg agattgtagc agggtaagtt ggcgttcaga
    17581 gcatttgact gagcagtggc gtggcgtgat taaatgtgac tcatatctga cattggctaa
    17641 gaaattctat tcagaccttc acttttgaga cgatatcgat tctgcaggga ataccaagac
    17701 tgatgaaaac aaaaaaaatc gaccatctat tatcagacag annnnnnnnn nnnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnag aattatgcgg gttttttatg cgttgctgct tctatatacc
    18001 tattctatag ggctatcatc tgttagttct tgacctctta gcggcatact tgacaaaccg
    18061 gatgatgcag atgctcgtct ccttttgctc ccatacttat atagttcaaa cttaataaaa
    18121 caactttgag ttacctatta tgtttgtcgt ttgtgggagt gccgccaccc cctgactaaa
    18181 catatgcgta tcatctatcg acttaacctt ctaataggga ggtccattct attgccccat
    18241 tctccccacg atcctaaata cattttgacg ctttcatagc cgagcacttt tagcgctata
    18301 aatgtattag cagctctata tccgccctga cagtatgtaa ggatttcggc atttttgtta
    18361 atacctgaat atatacgtcg taacttctcc ggatctttga aaaatccgtt atcgtcaacg
    18421 ttctttttcc aatctatgtt aatcgctgaa ggtatgtgcc ctgcgctagc tgcacgtaca
    18481 acagagccct catattcaaa ttttgaacgc gtatcgatga gtgtgactga ctttgtgttc
    18541 tttatcgcag atctcacccg cctcaggtcg gcaaggattt tcggattagc tcggcagtca
    18601 aaattagaat gagtaaaagg attggtcctc gtctctattc tgtttccctt atttttccat
    18661 ccttgatatc caccgtcgag gagggctact cttttatgcg agaaataaag cagtagccaa
    18721 actcctctag ctgaggaagg accagaaata tcatcataaa ataccgcaat cttctctttc
    18781 gttactccga tatttgagag tagcagcctc atatgattat taaactgctt gattccttgt
    18841 tcagagctat caatccaatg aaactgcatc aagtccatgt ttaccgcgcc tggaatgtgt
    18901 cctttaatat aatcggaaaa tggcctagta tcaatcaaga gcaaattcct tttctgtcct
    18961 tgatcataca ataattcgct caactgttta gaagcaataa caagttgcat gtcataacaa
    19021 gctgcaatca gtattttatt atcctatacg cggcgacttg gatataacta cccaccttaa
    19081 acggtattgt ctagcgatag aattaagaag gatgatgacc gcgccgagtg ccactcattc
    19141 gtagattaat ttggtaagtg attatggctg ttgttctgac atctgctcga tgtaggtgac
    19201 gggttgcgta tatgatacca gaatggccgg tcaaatcatt gcccattcat atccatgcga
    19261 tatgagcaga tcgctgatct catttcttga aaatctaatt aaacatccta gaccgcagcc
    19321 gcttatgtct tagttgaagt tattgtttaa tgcaacttaa tctcgctaaa accattaaaa
    19381 tgattaatgg cggcgctcgg taggactgac tgttccgtat tattcatatc ccaggagtgc
    19441 gatctgtttt ggtaaatggt ttacaagtat attgacatag taggaacttc gccaaaagga
    19501 actgattacg caataaaaaa tgctatccaa gaagataaga ctataaaaaa tatacaatgg
    19561 gccgaactag gcagggtcac cgtaaaagtt gacacacaaa cgttagatta ccaggcagag
    19621 gtcagaatcg gcttcaaggt tcaacgcagc agaaataact agcggctgct gcactacagt
    19681 aaaattcaca aatcaatcac aggtttatct atataagact ggtagcatga ctactgccat
    19741 catgtctgac agcattcttt ttttcaaaat acaaaaatac tttattcgct agagttagac
    19801 gaagcaactt cggttgagta agttttccca gtaggaattg tattacaatc tagtccattg
    19861 tcactgatct gttgcttttt tctatcgtgt ttagagccta actgcccgta atcgtctgca
    19921 actcccgcaa aaacatatct tatgcctttt cttgttaatt cggaactcaa catattattc
    19981 atttgttcta catctttctt caccttctcc tcagagaatt tgttcgttct agcaacaacg
    20041 tatttaaaaa catcttcctg atagcagtgg atttcataaa gctgagccat cgttaaatat
    20101 tcaaactatc ttgtgaaaag agttgttagg ttaagaaaga tttccaattg aatgaacaaa
    20161 ataacgcctc attcatcttg atatttgagg ggtgaggtaa gcataagtca cgttgaggag
    20221 tgaagaactg cggactcttt gggttaagac acacgccata ctcccgaaaa tcgttatgct
    20281 ggcaactgta gactcttcta atatcacccg cattcacgac aaatttgttg tatcgttagg
    20341 tataatgacc ggtatcagtt caaggagtaa ctgctggaga ctcgcgatta cctgtccgtt
    20401 ctggaacgca aacaacagga agtagaataa cagatgctat caatcttgat gttacgttag
    20461 cgatgcacct tgattgatgc aatacactct gagataattc tagcggccag atttgccgta
    20521 gtatggttaa aatcaaaatc cggagaaagc tcgactatat ctagtccaat tacgccgctg
    20581 tctattgcac gctttacgag atatatgaga tcgatactcg ataggccgcc cgctgacggt
    20641 aaagagactc ctggagcaaa agccgggtca atgcaatcga gatcaataga tacgtatttt
    20701 ttaaaccctg tcgcttttgc aaccacttta tctgcaattt tcaaaacgcc caattcgacg
    20761 aaatccagtg gagagacgat ctctaatcca gcattctgcg cattctccaa ttcctctggc
    20821 tctgcggccc tcgttccgat tagcatactt tttttatatt ctatatacgc agcagaatcc
    20881 gtaagtactg aaccataata gtttttcatt gaggaaacga agtcaggatg tgcgtcaaaa
    20941 taaagcagac cgattttacc caacacgccc cctatcgcgc gtaacgctat agttgttata
    21001 gaatgatctc cgccaatgat aatcggtatc ttcttcgctg aaactatgtc agctatcatc
    21061 tggggaagct gttccctagt gacgttacct gcatcgagga tccgcttatc gtcgagacta
    21121 ccacgcatgg gtgatactgg gatagttttt tcttcccttt caaaaaactc cgagtcattg
    21181 gatgccgagc gaatgatatc cggtgctcta ttcgttcctt tccgcttcgc tagcgacttt
    21241 gactcgtccg gcacaccaat caggattata tctgcctcgg aaaaggaact gtctctgata
    21301 gaacgataaa aacgaaccat ggcctttcga ttaatctgta ttcgaattct ttccctgtat
    21361 atataggcat tatactgctt tgtcatatac tagcttcctc atttctttac gcaagtcaag
    21421 atcaagatca cgtgaatgtc aaccttggat taatgcaatg agtgctgtgt catctgagga
    21481 aatcttttct tgccaactaa taaggagtgt tgtatattct agataataga taatcattct
    21541 cattaccaga ttaatgaaac aatcaataca agggtgtttt tcctcataat ttcatcagtc
    21601 cgtgtctgac gggtctatat taagaataag ttaaatacga aaccttgagc ccaatctacg
    21661 tttagttcca gggattgcct tggttacagg tcccagtaat acggtcatcc tcctaattta
    21721 gtgaggcggc tcttgggaaa atctaatata ctaagagtct ataccatatt gcatggtatt
    21781 tgacgcgcta cttcagcaga tcgtcccggc aacctgggag agctttgggg tgtggatccc
    21841 gttgacattt ggtctgagcg taggattagc atacgtaatt gtatcagcca taaggtctca
    21901 taagaaatag acctcaagcc catacttttc ttttcctttt tttctatagc tgaatattat
    21961 atgtctaa
//
LOCUS       DAOR01000011            6268 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_36416, whole genome shotgun sequence.
ACCESSION   DAOR01000011 DAOR01000000
VERSION     DAOR01000011.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_36416"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5529..5846
                     /estimated_length=318
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1936 a         1071 c         1099 g         1844 t
ORIGIN      
        1 cacttaacac ttaacactta acacgagaaa ttgataaata aatgataaga actttggttt
       61 gttaacctat tacgagagaa cctcatagca tgttaacaac attactcttg acttgcagat
      121 ttcagtaata gcacaggaat ttatagttcc agtggaaata atggggttga attaacgaca
      181 ttaacattgt taactaacat cttaacactc cagtggaaat aacggggtct ttcgtttcct
      241 tttttaacct tctacaaaag taattgttaa caccttgtta acatcagatt ttcatttatc
      301 agacccccac cccactattt cgtttccata tacttcacat acttcctcat atggtactat
      361 atctaaatga ataatattat tcaaataatt aataataatt gatttaggta tttaaaagca
      421 aaatgttaac atgttgttaa ggtaatataa aataatatta aatgtccttt cttatgttag
      481 aaaggaaaga taggggtgtg tgtgtggata ttgagctgta taggagtaat aaaaatttga
      541 gtgacgatct agttgatagt atatttgaaa aagctgtttc aggaaaaaca ctcattaaga
      601 atcgccggac attaactata gactacgtcc ccgaaaaact tccgttcaga gatgaggaaa
      661 ccaaaacaat cgcacaaacg ttagctgtgg ttttaaaagg cgcgagacct tcaaacttat
      721 tattatttgg gaaacctggt accggaaaga ccgcagtaat aaaaaatgtg gttcaacggc
      781 tacatcagaa ggctaaggac cttgggatag aagttacagt accgtttgta aacgcaaaaa
      841 gtgcgaatac cggatataaa gttctctatg aaattgccga gaatatggga attaatcagg
      901 aagaagaaaa gaaattacaa gtacatttta ctggcctgtc gatgggtgaa gcgacagata
      961 ggattcttga tttcattcaa aagaaaaaaa agcttcatgt tgtcctaata attgatgaag
     1021 ttgactcact ggtagagaga aacggcgatg atattttata taatttcaca agggcaaatg
     1081 agcaaatctc gggaggtggt tttattagtt taatcggtat atctaatagt ctcacgttca
     1141 aagataaact ggatccacgt gttcgtagca gtctaagtga agaagagatg gtatttaatc
     1201 catacacagt ggaacaatta caaaagatcc tagcagatag agccaagctt gcattcaacg
     1261 atgatgttat ttctagtgca gcaatcaatc tgtgtgcagc aatggcagga agagaaaacg
     1321 gcgatgcaag aaaagctatt gatttgctta gagttgctgc cgaaatcgcc gaaagagaaa
     1381 gattaactaa tgtcgaagaa aaacacgtcc gattagcaca agaaaaaatc gaaaaagata
     1441 ccaatcatga agttttaaaa aattccacta cccatactaa aataataata ttggcgataa
     1501 tgaaatcaaa gaatggaaat actggtgaag tatatgatat ttattcttca ttgtgtaata
     1561 atgctgaaca tgagccactt acacaacgcc gtataacaca aataataagt gaactcgacc
     1621 aactgggtct agtatcaagt gacgtagtga gcaaaggacg ttacggcaga tcgcagagaa
     1681 tcaaaattac ggttcccacc ttgaccatca gagaagcatt caaggacgat ccttctcttt
     1741 ctacatggga tttggttgat tgaaatccat ctgaaatggt tgaaggttag ctagattaac
     1801 tacaattgca attccggggg ttggaacaat acccagcgtc tgttgatatt tggtttgcgc
     1861 ttgaaaagcg cctgaattaa cgatcaaagt tccgcgataa ttttgaacat ctatactatg
     1921 tacatgacct gaatgaagta tgtctggaac ttcggtaatt accatcatgt cctcgagttc
     1981 cggtcctata ggtgtacgct ctccataaat aggtgataga tggcgtgctt tgagtaatat
     2041 cttcattgct tctgccggct ttgaataact tagacctgga gtcgtagcaa tgatatcatc
     2101 caagctctga ccatgataca tcaatatttt tacaccatct aactccacta agctagggtt
     2161 tcctagcatt gtaaaattct caaaagaata cagtttgtca gaatattttc tcggcagcgc
     2221 cggttgtggc aatgctcttc ttcctgggtc gtggttacct ggaattacaa agacattgat
     2281 atgttgtgga attttggcaa acaaatcaac gacgtgactc atttgtttgc tggtatccat
     2341 ttcaattaat tctttctctt gattaggaaa tatgccaata ccatcaatta agtctccccc
     2401 tatacataca aatttgatct tgctgacgat ttcgtcatca gttaatgaaa gccaatttaa
     2461 aaatctaaga aattctactt cttggaagta tttactacca acatgaagat ccgaaattaa
     2521 gaccgcatac gattccgatt ttgatcggtt gggaagatga tctggaatgt ctggagaaac
     2581 taaattcctt acaacaaaac cttgcattcc ttttttatta atattgtcaa cttctaccat
     2641 aatcatttgg tccagtgcaa gcattgaagc ctgttttttc aactcttcgg aaactgcaag
     2701 tacacttagc attccgctgt ggtcatcaat caccatctcc acaccattct tttttgaacg
     2761 cttggacatt aacaaaccag ctataacgga ggaatttcta ttcgcatctg tattcatgtc
     2821 gtacgtcttt cttaactttg cgttattaga tttatgcttt agagatgtaa tttttgtaat
     2881 tcgtttacta tcaggacgca atgctaagat acgaagtgat ttttcaaacc ggctgcggaa
     2941 aagtgcggta tagcccctaa tgccctctcc actgttaatt ccaggtgtgg gatctaaaat
     3001 cactttatag gaatgctgtc cagaagaaaa agtagcatca gcagcggccg tcgtaacatt
     3061 aagtagtgta ttactaaaac tattaacatc agccaccttt ccttcgggat taataagatt
     3121 cttgatatct tcaactagga tggaagagct ttctttgtgt ttgattttca ttttaactat
     3181 gtcttgcacg acctttaaaa tatcgatatc aagtcctttg agcatcgcaa aagcatcagg
     3241 atgtatttga taacctttgc taattgcaaa tgacaaagcg ttagaaattt ctctttgcat
     3301 agacacgtgt tgccattttc attatggata tcatgtttaa ttatatttct ctacttgttg
     3361 atataataca tagttatatg cctggctttg atttatttct tgaattcaaa ccatctgtgt
     3421 aaaaacaaag gaaattaaca tcaatgatcc tttatttgaa agaaagatag aaatcgcaac
     3481 tgatggccta agtcatgatt gttttaactg gctttataaa aaggtgcaaa actccaaaga
     3541 aaacgccgcg attatagcag attatgtcat ctctatgaag actatcaaag tcagagtata
     3601 atgtcattat gtcatggcag cgagggcatc aatttcctga aatcagtgct gtcgaatgta
     3661 taatttgtac cgccaaatac gtttactatc tggtatttta gtttttaatt tattcggtgc
     3721 aatttctatc ccctaagagg attttaatgt attttaatgt aaatgatcat ggtcaatcga
     3781 aagactttgt atttgcctct gagaaataca atgtcaagac catcgttcct actattccaa
     3841 ttgtttgtgc aaggttaagc atgtcagaaa atcctatctc tgaaaccaga gatctctaga
     3901 gaagttaaaa caacatattt aggaatatgt tggggctgcc agaaaccaaa tgggaagaca
     3961 agggagaaca acagcaagaa ggaaagtatc ctcagcacca cttagactta aagcaaagta
     4021 cgctttccga attcctagat taaagccatt caacagaatc gtggcaaatt tgaatggtat
     4081 cattttaaca ttttaatgaa taattccttg agactgtaag cctaagcccc ctctccctga
     4141 cattctgtaa tggatatatc gataatgatg cagaattcaa ttctatatgg atgaattatc
     4201 agagctagaa gaaagaatta aacagaatag cgcaaataaa agacgaaatg cacaagactc
     4261 gcgagagagc aattacggat agactatggc caaaaataga gacattgagt tgggtagtga
     4321 atgaaatatt ggtattaagc agagaggaat agggattttt cagctacacc tgtaatcctc
     4381 cggaacataa atttgtacac ttttattttc cctctttttg taattcgtaa aaatttgata
     4441 ttagtagtca ggaatggctt tggtgcgaac agcgactaga agtactaaaa atatgcttgt
     4501 ctgtctttat ggtgctactg gctccttctg gaatagacat ggcaacgtat gcttcagggg
     4561 aacccgacgt aaacactaga tttacctttg ctgatggaga atttcaattg attaagcata
     4621 agagcacgca atgacttttt aagagcaatt atgagttcta attaaatcta ttgtgcgttg
     4681 aacaatgtat ctgtttacaa atggaacatg gttacataaa ggtttaatat tctcgcgtcc
     4741 ataaaaaatt atgagtaaaa atagccaggt actaaaaaac tacaaacaat taagcatcgc
     4801 aacattactg gcattcggat cttgcttatt gatgatttcc ggatctgccg ttgcaccggt
     4861 ggcatatggc cgatcgcatg cggaaggatc agctacacta tcttcaggcg aaatatcgca
     4921 tgcggaagga gttattaaag ctgatacatc tcacgtggca cagtctttga tttatcttcc
     4981 acaagtaatc cggactcact aagttggtct tcatgaaaac gttgttgtgc ttgattatga
     5041 tgatgagtac acaaatctaa ttcttaagca taatgagcta tgaaacgatt gtctcttagg
     5101 gactactcca agtggtgatt gaaaaagtac tcaaacgtcg tctttatttg atacttgtct
     5161 gtggctcatt ttatcaactt gccacctaca ttaaatttta tttggtgtct cagcctcagc
     5221 agtaatacaa tcagtagaac catagactgg agcagcaaag ctggcatata ccattatttt
     5281 ctcgagtaca actactgctg tatgatcgaa aacagtgagc tttctacaaa tagctgagat
     5341 atggcttagg acggcatctc ctggtattag gcacctgacg ctgaaatatt ctcctaagga
     5401 catatctttt ctccatcttg gtagccgcta acgatattac tgcttctact ctttttttta
     5461 cccaatacct ttcttctacc cattctctca tagacttcca gtaaaagtag tgttggtaca
     5521 aaccctgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnatta gattaatcat gctcaagctt atttcataca
     5881 aatcataatc tattattagt cttcagaaat atgatcaact caatttcctt aatcaaagat
     5941 agaataattc ttcattgaaa tcggcttcta gagttagttg gatttgaaaa gaatcttgta
     6001 gtaacagtat acatgtatag tttaagatct aggagacttc ctcgatccaa cctgctattc
     6061 ttcgtattgc ttttaagcca cctgcgcctg atttagtcat taacaccagt cagcatgaca
     6121 attctggagt atattttatg actagataac ctaataagat caaagtgcat atatttagtc
     6181 gtatatctag ctaatctcgt gagcagtagc gacgtaataa aatagatgca tattcttatg
     6241 cgattatatt tgctatctgt ttttgtca
//
LOCUS       DAOR01000012            8410 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_27409, whole genome shotgun sequence.
ACCESSION   DAOR01000012 DAOR01000000
VERSION     DAOR01000012.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_27409"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    7227..7456
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2618 a         1511 c         1555 g         2496 t
ORIGIN      
        1 aaatagaaaa acaaatgttg taatcatgtt tacagatgtc aataattcaa cagaaatgag
       61 cctatcatta tgggaggata ggtttgcttt actgattcag acatttgctc aagaaataag
      121 tatcgctgtg accggttatg gcggatatgt cttcaaatat gaaggtgatg cggttatcgg
      181 actttttccc ggagaatatg atcaggcaaa ggcatgcaaa aatgcattaa attgtacgac
      241 ttcaatactg gagatcatta aagaagtaat aaaccctgcg tttaaggaaa atggtttacc
      301 tgaaatcagc gtaagaatag gattgactta tggttatgct ttagtagtgc tttatggaaa
      361 cagtttagaa aaagcttaca tagatatcat tggttccagc ataagcttgg cttcaaagat
      421 cgtctctatc gcgaaaccta atcaggtttt agttggcgaa tctatctata atatcctcct
      481 gtcatcaaaa tctataagcg atagaaagtt tatagagata aatctcgatc caacaaaatg
      541 gaaatatctt tctcgttctg accctgaaag tatgtatcgt gtatacgaat accatagtta
      601 accgataatt ataaaatgtc tacttttaga aattgaatgt aatgataaac cgcaatccac
      661 atgacatagc acttgtaaac tgaattagta ttatgtggga cacaagctcg ctttttacac
      721 caacatcctt gggtccaccc actcaacaaa cgaaacctgt acgtaatgga taacaggcat
      781 gggcagtggt ggggatccag ttgcaagttc accacccgta gatgatgtcc ctgctgtaca
      841 caggtctaag attaaatctt taaacttagt tgcaatgttg aggtctttga ctcacgccgc
      901 ctgaaacgga tgctcgtttt cctcattgat agtgtggtat acacttaaaa gatcagacgc
      961 cgctatcatc atcgttatga ctactataac cgactctatg aggaaaaaat tagaaactat
     1021 tgaaaaatca gcttctgttc aagaagcagc taaaaaaatg acagataaga atgtgagttc
     1081 attaatagta gtaggtccag atggcaaacc acaaggtttg gttactgaac gtgatttagt
     1141 taaaaaggta tgtcttaatg atcttcgcac tagcctagtg actaatgagc agataatgtc
     1201 ttctcctctt attactatcg attccaaatc atcgccgtca gtagcagctg atatgatgct
     1261 gcaatataat gtgaggcatt tgcttgttat tgataagtca gacgaaaaca aacccgttgg
     1321 tatagttact ccactcgact ttacaagata ccaggaatat ccaaacgatg aagttagtaa
     1381 agatgctgtt gaaaagatac tagaatatta tatataaata tgaattctat tgcttctact
     1441 atcaggactt acgttggtgg ataactgaca atacaaacta ggatttatgt actacgatac
     1501 ttgctagggt attaatcacg acttaccccc ccaacgattg ttatttggat tttttcttat
     1561 tctcagaatc aaaattgacc caatattgct actatattct gtactatctg ttttgagatg
     1621 tgggtcaagc ccttaagaag gtacgtctct tgcagagggt tgcgaggttc ctcatcgatg
     1681 agtggatact ggtcggatgg cgatatatgc aaccaaaatg atgatcccga ggaattcgag
     1741 ggagagtgat gtcgtgaaca aagagattat ctctttgacg aacgatggaa aacgtgcttt
     1801 gaaattacta gcggagattc gaatacttgg gaatagacga acttgtacac atttttttaa
     1861 cttaattggt aatgatttgc tttgttgcca ttaactcaat ttggaagcat aatatcttga
     1921 caaaaaatag attttatttt tttgctgtta tcgttattgt ggatcggtta taattatgca
     1981 gtctttatga gttttcggaa gtgctctccg ttcttcgcaa tctcttcatt ccaagtttct
     2041 tccgaatatc catgaacttc tattccgtgt ttctttgcat tctgtaacaa ctcttgctca
     2101 gtttctcctg tatattgtaa attttttgac gctcatgcat caggctatct gccaatccta
     2161 gtcatttaac actaattgca gtagtaaata gtagaatgga tagacaacta actttagtta
     2221 tcggatccaa ttcaggtaag aaagttgcag atattagccg tagacaaatg catctttctt
     2281 tcgtttgcta tgattatgat agttattata acggagggat attcaaattg ctcagttaca
     2341 acttatagga tttattgata attctcttca tcatgttagg agattaggaa ccctagttag
     2401 ccagatatac gttcctcgtg caccttctta atgtttaggt aatcacgccg gataatagat
     2461 tccaaaccat tatgccttga tctgttcttc ctatatttca taaatgatca ccatgactta
     2521 tcatcagatc tgttttcgtc gatctacaat tgtttacgct cctcatcctt agaaggtcta
     2581 gcaatcacac ctgattcacc ttcgtattct tctgcaaaat tttaaaaaga atggaacttt
     2641 atgcggcact atataggcac actttggaca agaatttttt gttgtattta gatctcaaaa
     2701 tggcaaccaa aaccgggtgg gtcataaaga aatcaagaaa tagaccgcta ctccagctga
     2761 gacacaacac ggagaaaaga gatacataca tcatagctaa acgaagaatg cgataggagt
     2821 aagtaggatt tatgactaat cgttccttgt ttgttacgtt ccacctaaaa ccataactat
     2881 accaacatct tccgttctca atccttatct tgtcacagta gaatagttgg gaggcaagtt
     2941 atattccaaa aacaccattg tgctttatcg gtggcctcaa tcgtctatat cagtatgtat
     3001 cgaatggtct aaaacaccat taaaagccaa taatatgata tgaaatccac aattttttcc
     3061 tatttccaat tctacgtgca gaagctagga agctatctac acaaattaat atctcccttg
     3121 gtttggcatt aagctctaga gttagaatag gctcttgttg caaaagttat tataaaaaat
     3181 aaaaagggat ccaataaaat caaaaaacac gtgtccgtcc aattagaggt ttgtctcaaa
     3241 ttcttccgga ctcctacaaa tcctttctca ggtggagtat ctagtgtcat tttactgtag
     3301 tcttgagttt aaagaaatga aacctgctct tccctactat gcaatttcgt agtaagcggg
     3361 cctaattgtt gcactctatt atgaatacgc aaaaaattaa tgcccttctc cgtagtcttg
     3421 aatgttcttt ctccgtccaa atattcgatc aggcctcttt ctattagaag taccagatat
     3481 tccttagtta ctgcgtgtga aaggtatccg tcatacatta ttttcgttag tagaactcta
     3541 ttgccgttag cactttcaag aatctgcgca gtaatttcgt ttctatctct atacttcata
     3601 tctatatcgg taattataga atatataaat gtatacttct atacggtaag tacgttatat
     3661 gcttaagcat aaagaaatgt acctgtgtat ataaatgacg tatccatatg gtaagatctc
     3721 catatgccaa agcatatata gatataagag tgcgtataag aaaacgtgta tatggaaaag
     3781 gaagaaatgg tagaagatga tgaaactaaa accacgatga aaattcctac agatctactt
     3841 gatgatttca aacatttggc tatagatatg aaaacgacag tgactgccct tatcgtagaa
     3901 tcaatgaggc tgtatcttaa agatattaag aagaaatgaa ccagattctc gtggaagggg
     3961 gaatactctc cgcaagagca aggaaagata agcctattct ccaaattaag caaatctgaa
     4021 atgcgcgctg cttatcgctt cgattacaaa gttgagatat gtcatcaagg ggtttaagat
     4081 ctccttgatg tattgtaaca agatagtccc ttcctgctaa gatcgataat tgactgaatc
     4141 tagacaaggt cttctctttt tctgcagtag ttggaaaatg aagtataatg aacacatgat
     4201 cctcatatct gtctacctta ggaatatgga tcttggaaag acagtcttct acatttagtt
     4261 catgaaaatt ataattcttg gccagcagag tcatcttctc gttagtaggc ttttggatat
     4321 ctgtccacaa aaaccattat tgcttattgt ctctagatct cttcgaacct gttccgttca
     4381 taacagcttc ctccatttga atgtggaatt ctggtttata atctagttct gttttatccc
     4441 tataacttgc tagaaagtgg caacaataac gacgatgaaa ataacgagag gaaacaagca
     4501 caaatacctt gacgacctct tcaaatatac tgagtgaatg aatgagttaa tgagcgagta
     4561 tacctctatc tatattttag ttgcaatcca cacatattaa gtcgcacata ataagtttag
     4621 atactcggat ttgaatgccg ctactgcgac ctatcttttt cagcttatat cgaacgtgta
     4681 aaacatatag atttagacca tgtaggtaag ctatactatc ctactcgtga agaatttaag
     4741 gatagattaa aaccgaacgg gaaacgatga gcgaaaatct cgtctgcaga ggactagaaa
     4801 agagatcatt tattcgatta aaacccagca acaggatttc gaagtattcc tagcagagga
     4861 atttgagatg gatgatgggt tttcatggtc ttcctccccg ggaggccaat caaattgagg
     4921 acaattagaa tcccactagt aaatctgctt ttagcgatac tattttcaga acatatgcat
     4981 tcgacattca gcctggtagt caccagcatg ctattgctag caagtgccgc taccgtcact
     5041 accgctacta ctaggtcgga tctggtccac taaagctccc gaaaccatag actagaacaa
     5101 aaaacttcca ttcaaagcat gggggagcat atagcaaaca gtaaccctca atacttcagc
     5161 cagtgttaat catttcagga gctccctata ttcacctgaa tctctaatca gatgattcaa
     5221 aacacaaaaa tcaaatatac tctatgaggg cttgctaggt atgtcggata cattaattcg
     5281 tagcaaaagc ttcacttcgt ttttcctagt tttgatctca ataaacatta taccgattgg
     5341 ctacgctatt aattctgttt atggacaggc ttcattttct ggaagccagc caaccatcag
     5401 tgactctaat ctcaaggcag agttagtttt cgagggactt aaaagtccta ccagcatggc
     5461 attcttaggt ccaaatgata tgttggtttt ggaaaaggac caaggtaccg tccagagaat
     5521 tataaacgga aaaatgatcc cacagccatt gcttcaagtc aacgttgcta ccgatggaga
     5581 aagaggtatg ttaggaattg ctattgcaaa aaatatcacg aatggaaccc catttgtatt
     5641 tctttattat actgaaagta aacaaatggc aagtccgcct ttaggaaatc atgtctatag
     5701 atatgaatta gtagataata aattaataaa tcctaaactc attcttaacc taccagctac
     5761 ccccggtcct tttcataatg gtggaaaagt tctgattggt cctgataaga atgtatacgc
     5821 tgttatcggt gatcttcttt atcatagaac ccaagctcaa aatgtggcta ctggaggacc
     5881 gcctgatcta accagtggaa taattagagt tacagaagac gggaagccgg taccaaatag
     5941 tcccttagga tctacttatc ctctaagtct atactacgcg tatggcatac ggaacagttt
     6001 tggcatggat tttgatcctg taacggggaa tttatgggat acagaaaatg gacccaatta
     6061 tggagatgag ctaaatcttg ttgagcctgg ctttgacagt ggttggttac aagtacaagg
     6121 catatggact ccaaatgggg ccatagagca cgagaatgca ggaccaatca atcttcatcc
     6181 acttaacttg gtagaccttg gaggaaaggg aaaataccga gctcctgaat tcatatggtt
     6241 tcaaacagta gctccaacag cacttaagtt tctcaattct acaaaacttg gaaaacaata
     6301 tcaaaatgat atctttgtag gagattacaa caatggcaat ctatatcatt ttaaactaaa
     6361 ccaaaataga acagggctac ttcttagtgg cacactagct gataaaatag cacaaacacc
     6421 acaggatgct aaaccagtaa tgattggtag tggatttgat ggtggaatta cagatttaca
     6481 agttggaccg gatggttatc tctatattct tacatttgca ggtgcactat atagaatagt
     6541 tcccgctagt tcgagttgac gtgataattt ctcttttaga ctggttggct tttagctgta
     6601 ttcccaaaaa attctgttag tatccactct cttataagaa ttattgcacg tataagtaaa
     6661 agatatatag aattttatta aggttgaggc tgcaagactt taagaggttt ggaaaaaagt
     6721 gctattaacc gatatagatt gcctgtaccg agaaatttgc tagaaagagt agacagaacc
     6781 tcctctccag ctcacgcagg aaaccttcga aacgcaatag atttcatagt cccggaaaat
     6841 actctggtac tcgctgcagc caatggtata gtcacttatg ttaaagacga ttctatcatt
     6901 ggtgggccta atccaatcta ttggaacttt acaaattttg taactatcat gcatgcgaat
     6961 gaagaatatt caaggtacga tcacttggca cctcaaagct caaaagtcaa agtaggtcag
     7021 gttgtcagcg aaggacaaga gatagccaga gtcgggatga caggatatac attccatcca
     7081 catctacatt ttcaggtgtt catttttact agaactaata tatggataga ttttgatacg
     7141 gtaagtgttc aagattttat ctcatgaaat tatttgtaca tcattgaatg tgtcagcttt
     7201 tatttccact ttcctgctgc ttcatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnntcaa gaaggcaaag gcagtctact cgattctagt cgtttatttc
     7501 attagaaatc aaatacgagc tttatatcgt attctgtcca attccacaca taggctggtg
     7561 atatctgttt gtctgcaaaa tccagtaata accatgggcg aggcgacact gcaacaacgg
     7621 acgagccgtt agaagaattg aatagacagt taatgccgat ctgtttattg atgtaaagat
     7681 atggccaacg aggacttcaa tgctacgata aggaactctc agcggcagac catattgaat
     7741 ttgtataatt cagcaattcc aacggaaata atctcctttc agctagatat cagccaagaa
     7801 gaagttgaaa atattataga tgaagagagg aaggaagagg aaaagttatc acttaaaggg
     7861 gcaacgccac cggatgcttc ttcttctatg gatttatttt atctcgatgc catcgttaac
     7921 attgaccttg ctatcaaaca cgcgcagaat agtatgtggg tggctttgaa gtcagaacca
     7981 aagtttgaca tttcaatgga agaaacagat aaaattttag agaaatttgc taaatccaaa
     8041 gttactcttg taattttgca tgttgatctt gtagactcaa ccaggctgtt aatgacactg
     8101 ccagttgata gactctcaac gataattcag gcatttactc gagaaatgtc tttaataata
     8161 gaagcatacg gaggatgtat cctgaagtat attggagatg caattttagc tttcttcact
     8221 gtaaacataa aagatgagct atatttgcca tgtgtaaacg cagttaattg tgcaagatct
     8281 gtgattaaga taatccaaaa tggtataaac ccaattcttg atcaaaatgg ttaccctgag
     8341 atgagcgttc gtattggcat agatgtcggt gaccagaacg cggtattgca gtatggttgg
     8401 gatattattc
//
LOCUS       DAOR01000013            3903 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_67613, whole genome shotgun sequence.
ACCESSION   DAOR01000013 DAOR01000000
VERSION     DAOR01000013.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_67613"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1268 a          757 c          697 g         1181 t
ORIGIN      
        1 tacgaacttg agccttttgg aatcaaagta atattgatcg agccgggtgc aattggcagt
       61 aatttcatga agggaagtgt tttgcctaag agagcgctag atccacagtc gccttactca
      121 gaattagtgc aaaaatttag cgttaaaaca agttctcagc atgataatgc aacacagccg
      181 gaggaagttg caaagactat attccaagct atttcaattg aaaagcctga atttagatat
      241 gtagttggca acgatgctgt tagtttattg gaagcaagaa aaaatatgcc atattcagag
      301 ttccaaaaga tgataataca aaatatcata caatagttat gcatatcatc aaatatctgc
      361 agacgatatg tagataacaa aataagaaca atatacctcc aatttaagag ttctaataat
      421 atgcctaggt cagttcgaac ttcctgtagc agctgctaga tgctagcggt ttccacataa
      481 agtcggccaa tatttttctc ctctagatta gggaacttgg tttcagtctt ggtctatttt
      541 aggtaaagag catacaccat gtctcttcaa cgtaatccta tgtttatctg gccaatttct
      601 aagcttcaga tacgtatgca tgcatacatt acatcgtaca ccaatccagc ataaagaact
      661 gtagaataga tttacgtttt ctagtgaatg caaatatcat ttcatatcat atgagtaaac
      721 attgattctg gctctgtttt aaaccgggca aaaagctttc ccagtcttcc tcatgactaa
      781 ctccatctgt aagtaaatct tcgaatattt catgagtaac tagatctttc atatgatatt
      841 tttttgcttg tttgatatat ttttcgatta cacaactttc accttctatc accagttcca
      901 cgattctgta tcattagcca tggtattgtt taggctacag tagcggaatg tgcttacttt
      961 atgacgattc gtggcgctga cggtcttttc tttatactac cagcaagtac aatagatact
     1021 actgagagca atgtcgctgt aagaaatatt agattatatg attgaggagc cgggaatgaa
     1081 gaaccactga tgcccttaac gaacgcttga tttgcttgca taaatattcc agctatgact
     1141 ggaccaacag aggtcccaat tagataaaga agagtagtca tcccaagcga tactccattc
     1201 gactgctttg gagtattcac caaaatgata ttaatgctac cgatctgtgt taatgatagt
     1261 ccagttgcta ttacggcaag ggaggttgtt attaaaaatt ctgttgaatg taacgcaaag
     1321 agcaaaaaga atcctgcaat ggatattata attcctgata tggtagggct ttgattacca
     1381 aattttgata ctatgaaacc ggaagcaata gatactatta gtgatataac catgtaaggc
     1441 aattgaacgt ttgcgatact taatgcattt ccaccaaatc ctaacggagg tggacttcga
     1501 atcagtatag gtatagtctg atacactacc attaaagcag taatgccaac taccatgttt
     1561 atgatattcg cacgaagtag aactttattt accaacagct tgagatcgat taatggagaa
     1621 tctgcctttc tctctgtcat aataaaaaat gacagagaaa tagttgccgc agctccaaaa
     1681 ccaatcacaa tgggtacgtt tgcagaggct gctgcattaa ctgtttgtat gaattgtaat
     1741 gcgactagaa atgagattac tgtgatagat agtgttatcg cacccatcat atctatagta
     1801 gccttcttct tgttgatgct gccgctgcta ctatcatatt ctattgttgt actactactt
     1861 tcggaaagta gtatgtccct tctcacatga ataaacctgc aacaaaattc agtatttttg
     1921 ttagatattc gtgatgttgc tttgtccatg tcgatatgaa taaatctgct aattgctaca
     1981 aataatacaa ttgctattgg tatcaccagt aagaatgtag catgccatcc aaaattattg
     2041 attatagttc cacctattgc tactcctatt actgcgccag ccgaaaacat ggaactaaat
     2101 attccctgag ctaccgcaag tttttctggg gcgaatttat cccttatgat accaaatgaa
     2161 atagcaaaca ttgaggcccc aaagccttgc attattcttg caataatcat aactgtgaaa
     2221 tttgtagaga tagcagccac agaaatacca atagtataaa ttcccacgat tatgagcaac
     2281 attttcttct taccatacat atctgatagc cttccggcga taggagtcat aacagctcct
     2341 gtgattaaga acgatgcaag tatccaagaa gacgcattat aagttatctt aaaatccctg
     2401 ataaggtctg gaatagctgg aagtatcatt gtatctgcga acatagccat gagacccaca
     2461 caacttaaga ttgccagtgt tatccatgga gatctagaaa tttttccacc ggtttgacta
     2521 tttgtagttg gtctgctcac agtcttgatc ccttatcgtc attgattata tgtattgcct
     2581 tatacggagt gaaaaatata tctaaaacaa ctggatttag atgtttaggt ttacgattat
     2641 ttttgcacta tgatcctagt ctgccctttg acaaataccc tgctttgcaa aaagagtgcg
     2701 gcagacataa caagatagct tgaattatag aacaaatccc atatccactc ttcttttcgt
     2761 gctttatgta ttacggcgct atattcgaag ccaatatctg ctacagtgac aagcactata
     2821 gatagagcta acaaactcca atttgctgaa gccacattct taccccgtaa tacccacaaa
     2881 attaggacgg caggaatgag caagattcca tctaatattg gatacgtaat actgatcagc
     2941 catgctaggc taccttcttg agcagacaat aaatctgcga taccaaaagt gagattaagt
     3001 atatagccca agataatcgc ggcagctaat gatacaagaa ccactaacaa tttttcattt
     3061 tctttactta agaggttgta tattctaaac ataaaataaa taaaaaatcc atagccgctt
     3121 aaccaaaatg catcagcaga agacggaaat ggagtaccaa tatccaatac aagttgataa
     3181 taagtccaag tgacttctgc aacgagccag acggctattc ccacagtaaa aatgagatac
     3241 gcctgaacaa aaaatccatc tatcctgtgt ttgtaggcag taactaccgt tatgatagac
     3301 agtgacaacg cggtagcggc tgccgtgttt atggtccaat tagtaaagaa atctcgggtg
     3361 tcttcgcttg aaaggataat aaaagaattt gatactacta aggatccgac cagtatgagt
     3421 atcgttcgcc aggggtcatt tacatacttc cgataagata gcaatctaat ttccacactg
     3481 ttctctagta tcgatggcat cactgactgg cctaaaaccc gaaattcgaa taacatccag
     3541 taggcttaaa acatgtgcct cattatgata actaatgtgt tccaagagca ttacaccctt
     3601 ttaaatttgg atatcatcaa agatttcaaa gcgatttcga taacaagcat tccatgcagt
     3661 ccccttgccg tgcgtactgc cataatactc tctaatacgt ctactctcat atttgtatat
     3721 tcccatgaac ggaaatattg acatagatat tcccatgaac ggaaataggt ttaatacaaa
     3781 gtggtattat attatctgca aagatctatc cctaatcgga tattctaaac ccgatataga
     3841 tagtaggttt atgcgagaat ggaaataaaa tcgaagctat ttataataat ggtttcatta
     3901 gtt
//
LOCUS       DAOR01000014            9233 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_46823, whole genome shotgun sequence.
ACCESSION   DAOR01000014 DAOR01000000
VERSION     DAOR01000014.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_46823"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5359..5463
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6991..7194
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2877 a         1670 c         1851 g         2526 t
ORIGIN      
        1 atttttgctg actcgagaaa agaggttgga tcaccgccac tacctgccga gccttgagcg
       61 gtaacaggaa caggaacagc agctactgac atacttaata ttagtaaggc tgtcgttact
      121 cccgtaacaa cgattgcaaa ttttactcct attgtcatcc ttgctgccta aactggggga
      181 atttgtatat aaagattata tacttcagaa tgatgcgcgg tacgatattc gacaactgac
      241 cccatctctg aacgctgtgt attccgaagc tctcagcaag aagaggctgg gcaaggttcc
      301 agtcgaggag gtaataacaa aattcgattg gaggttgagt gttaatcacc atcttaagag
      361 ggggcacaac gattcgcaag tagtctgtag accaatttga aaaattttta gttgtttttc
      421 tgctactaag agaacgcaca gacttttggt tgcagtgcaa gaaaaagaac aacaactaga
      481 tctagaatct gccgaatagg cattaaaatt ttttacacaa accacgtcta attcgttttg
      541 gacacgagtc aaagtataag tgcacggagg aaggtgagaa tgctgagtaa ggagaaatag
      601 aaaaagagca attgcaaccc acctctaaat taggttatag aaaagaggtt tcggattttg
      661 gagactagtg ggatattatc gcaacttcgc actttcatgt tttttctaaa gatgctgagt
      721 aacaattgtt gacatacctc atagcccact tccgaagaat agttctaaat agaacactgt
      781 gtaagtagac acgtcattgc ccaagaaaac agtatatggt aaatgtccaa ggtgtaaaac
      841 aaaaagtgcg cttgaagtag tctctgggaa tcccggcaga gagaagttca agtgtaatca
      901 gtgttactat aactttggta tggacgagct ctagcacttt actggaccac ttaaattctc
      961 ttaatatcta ttaggtctct tctcgaaccg aggtgtgacg gcgttgaata cttaatctag
     1021 tactacatga tatcccaata ctcaggacaa aaaaattgtc aaatagtgaa actaaagata
     1081 aaagagacac tgaaagtctg cgatacgaaa aatttgtatc actcaaccca aaacaggcga
     1141 cctgtaaagt gtgtggattg acagctcgta acgaaacaga actggaagac catgttcgtc
     1201 atgcgcataa aaatgagaat aataacgtac ctggaaagag ctcataatct tgtctttttg
     1261 attccacatc gtttatcttc aagtcagata cacattggca agttaaacaa atataaaaac
     1321 aacatcgtaa aataagatat aaaataagat atgaaggaca agaagatgaa taattagaat
     1381 gccaatgcta atgataaagt gtaaaacttg tggcgaagtc tttcccgggc tgtacgttcc
     1441 agaaggcagt agtactgaat tcaaaacctc agctacaaat gcggaaactt cacacacctg
     1501 ttcgagagga cacaaaaatg aatatgtcat ggaagattat atggactggt cttgattccg
     1561 aacaatctga tctgcatatt ctggtttagc gatcctggcg agagagcttt tattggaaaa
     1621 aaagagacag actatagtgc ccaaacccac ttgatgcctc aaatgctttc ggccattctg
     1681 gccaaatatg gatatcttcc aactgagata aatatgccat aaggcatatt cgagagttaa
     1741 gaaaatcact tctatactgc gacaaattga tatccttgat gccattcggg cacacggagt
     1801 cccaatgtac cagaataacc aataggcgag tgagccgatc aataatctct taaaaggtac
     1861 taagtcgcat gagctaggcg attggcggac atccctgtct atccaagagt acattaatta
     1921 attatgaact gtcagacatc gatggggctg tttctataaa gtagaatttc tagaaatgaa
     1981 ataaatgatt taatttcaga ttatccgact gattctcgaa gacatgtggc tttcccttcg
     2041 tgactgcctt cataggttcc aaacagccat gtggtttgcc cttaccatcc tagccttgga
     2101 ctccacaccg tttctttcga actagcggtt cacctattaa tcctagtatg acaaccatgc
     2161 atttccatgg acgtactagg cctttaccta atacggaaat ctgcatgatg gatcatcctt
     2221 ctgtttcggt cagtcggaca tgatatatat cgcataacat cctattatac ctttaaccat
     2281 tcttactgct taaattattc gaaaatgagc gtggatgttg ggatcagatt tgctaataat
     2341 aaagcccgtg caattttttt gctcgaattg agtagcagat aacatgtcaa cacgtcatat
     2401 gcttctgcac tgatcttgca acgtgctcca gtacgtttgg tgcgtgcata tacagtactc
     2461 gcccccattc ctagaaatta accgaaccac caaccgctgt atgtatatct ggatctagtt
     2521 gttaaacaga aatataatat ttattagaac aagtacaacg tcgatctgga gtggatacta
     2581 tcgaatcaat aagaaaggcc aaacgcaaag ccgcagaact tgctgacact accctatcac
     2641 tagttaatag taaagaaata gaaactgcaa gttcctatta ccttaaagca ttagaagagg
     2701 taggtagaat aaaaattctc caggacaacc caggagatgg aatcgcaaag actgaaggag
     2761 cattcgttga ggatgcagat tatataatgc aggaggcaag agattccaga attcaggcag
     2821 ctctggctta tttggtaaga gaaaacgatg ttagcttcgt tgtcaaaaat ggaacacagt
     2881 tcgtgatccg aaaagatgtc ccggggaacg aattaaagag agcagtagta tctttaaaga
     2941 ccaaactggc attcttatag cctggcctga atcgaatatt atttaaatga attcgaattt
     3001 cggttttccg ggattccacc agactactct atgcctcaac tacttcggct gcgcctttaa
     3061 tgaagttccc gattattgcc gtgtaggtgg gatttcagta cggctttagt ggatcttcgt
     3121 gggcggctct ccataagttt gccgtctacg atgtggttta tagccgacga agtcaatcgt
     3181 ggttgtattg ggtccgaatg cgaaaaacga tgaggtgtcc gagtgtggat catactatcc
     3241 gaagtcaatg gaatagtcaa acctgatatg acatgaagga tatttggctc tttttattgg
     3301 aaatgcttgc tcaaagctga agtaagcaaa aaacaaatta aacgtcgtaa gtaagatatt
     3361 aatcaattat ttgtcagtac taaagaaata ataagttgtc atatcattgg gtccacctcg
     3421 tcaagggtcc ggctgtagtc acccttgaag gatttgggga agtactagga aaagacgtat
     3481 ctaataacca agtgtctgta ggagtcggca agattttacc atttgagatc ggctctggtt
     3541 gcaaaattga tatcaagggt ggtaagagtc ggctggtgtc cagttataat tttggaacag
     3601 caatatggca tcagattatt cgaagaattt ttctcgaaaa catgacaaca gtaagaaaaa
     3661 tattgattgt cggggatact gatactggga aatctactct agcaacttat attacgaata
     3721 aggcacttga aaaaggatac aggcctggca taatagacgc agatataggt caaggggatc
     3781 tggcccctcc aaatgcgatt ggtgctgctg ttgtcacaga gcagatcact gatctacgag
     3841 gtgtcaatac tcacttctat gagtttgtag gaaatatcag tcctgttgga tttgaagacc
     3901 ttattatcaa agcagttaaa aggattctcg caaaaattag aactgcctgt gacatttgtg
     3961 taattaacac agacggttac atcctcaaca ccggtattga gtataaagtc aggatggcga
     4021 aggaaattcg atccgatatg cttgtttgcc taggaaaagc atctctactt aacaacttca
     4081 aagcaagatt ggatccatct actgtagtat ttggaaggtc ccctagcaaa accaccaaat
     4141 ctagaattga ccgcagtaag agaagattaa atcaatttca acgctatttc aaagaacaat
     4201 ctagaactaa acttatcgcc aaagaattga gttatactaa gttcgtatat agaggccaaa
     4261 catactctgg aatgtggata gacagagatg gctttttacg attgaataaa agaaaaactt
     4321 tgccagtgaa gctgcgaaga ccggagggta tgtttgtagg tttaggcatg aatagagaaa
     4381 ttgttggatt tggtctcata ctaaatgctt ctcgccacga gttaactatc caatcaagtg
     4441 ctaatgactt taacaaaatt catcttagta actctggaat atgcaacgaa aaaacagtac
     4501 tgggattaga tacactgaat agactaataa aaaaagagta gatgctaaag tagaaagctg
     4561 ccggtcaggt cttatcatgg gagctatgcc ttggaaagga aacgggaccg agcaccattc
     4621 gtatgcatgt accttctgat cttgacattg gcctcataac ttagatcgcc tatcaaatcc
     4681 tcagtataac gatcatatcc gcattttatc tgcattttat ctgggctata ttgaactgtg
     4741 accataccat actcaatttg acaagaattt gaccagcagt gttactccac tgtaagatgt
     4801 ctataaatcg ataaaataca tgccttttgt tagggtatca aatattccta cagttgccct
     4861 atatccaaag aaccaacctt ttgcagttcc cggattaaga actaatgttc tgccaacctt
     4921 agaggttagg gttcgatgag tatgcccaca cactataaca tcatattttc ctgattcaac
     4981 taacaaatct ctgcgttctt gtcgagttcc atggtatact gcaaatatta agtcatcaat
     5041 ttcaaattca cacaaatcac ctttaatttg accacctatg tcgctaaagg cgtctcttat
     5101 gccggattta tcaaaatcat tatttccaaa aacccctact agttttaccc cgcgaaaata
     5161 tctaattgct tcaggactaa catagtcgcc cgcgtggatt accaaatctg ttttgctctc
     5221 cttgaatact tcgacggctt tttctatatt ttcgatatta tcatgggtat ctgctattaa
     5281 gccgattttc aatattgttt tcagtctatt ctgtgttcgt taaaatacct atgcttattc
     5341 tctgatcccg tgccaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnntatgatc ccgtgccagg acttgttttc tggatcatct atatttcatg aataaatttc
     5521 tagcaaaacc agatacacat tattcccaga cgagagtggc ccatgcattc aatactgaca
     5581 tcttaaatat gcagcagtac ttgtttgaaa actaaatcaa cacatggata agtacatacg
     5641 tttatttcca tatgtgtcgg ccgctttctg gctgtttgga caaatacttc gccgtatatg
     5701 ttggtttgga gggatttaaa attatagccg cgctaagaaa ttaagatatt tcatttttgg
     5761 catacgtgta agccaagcca aaaattacac atatctctgc gataggcacc atccatccaa
     5821 ggcccattct tgtaaaacag aaagtcagtt ctgttttaga gctgtcgcag ctgtttgtcg
     5881 ccacaaattg actgaaggaa gacttgttta atatcgcatt taagaacgca ttattaggtt
     5941 ttcatcttgt gtcagatttc accacacggt tacgcagtag ctataatgga ggtgctatag
     6001 ttgacagttg tattagagtc tgatgagcaa cagcagcaga agactactaa ttgtgctagg
     6061 tgtgataggc agttgaattc gattaacagt tacttttgtg ctatttgcgt ggttgatatg
     6121 atacccaaaa tctacccatg taagatttgc aatagagaat attcagcttc aagtgtcgtc
     6181 aagttttgta ataattgtat tcctattgat tttgccattg attctgcgga gcttaggagg
     6241 ctatgactac taccaataaa tctaagatta cattagaaga ttgtcattgc ccacttggta
     6301 agtacaccaa tcagaagggc tatgttgtat gtgtatatgt ggcaatgtat cacgacctag
     6361 attgcccaat tcgtaagcgg attaactcta gtgagtatgg ctataatacg taggtgcttg
     6421 gagctgggaa ttaaattatg ttagatcatt ggtggccgat gggattgtat acagcaatgt
     6481 tcaatattgc acagaagcac tctacagtag aaagaccgtt aggagcgaaa gaagtatcag
     6541 taactattgt cagagagtgg ctaaaagaac atactggatt tgaatgtaag cacccagaag
     6601 aaaggattaa gatagacaag gaagaagaag gctatcccac gatagggcat tgcacatact
     6661 gctggacaag gtttgaagta ataaagaaac gtggtatgaa gaggaatgtc aatgagttca
     6721 cagttgtacc gggaaagtat agggagatta agccgcctat gtttgaagaa tggagagatg
     6781 ttctacaaga aactttagga gaaaacatag aagtatgact tatccgatat ggaagaatct
     6841 tctatactta tctgactacc atattataca ttatacatta tatgattata taatagggtg
     6901 tatttttgcg catgttagga attcattagg agttaagtta gaggttaggt taggtaaggg
     6961 aatgagcctg agtgccgcag gcacgaaggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcacga
     7201 aggcgagtct gtcgtgtgat agtgatagta ggaagaagcc caagccatta cctagcccta
     7261 ttccaccatt aacaggaggc agtatagtag ttaagtatga ttaatcagat atttatatta
     7321 ctggtggtat taatgtcatc tgtgtggtta gtaaggcaaa ttcagacttt acaaagttcg
     7381 agtggaaaca ggggatctaa ttgactacac gaaggattct taagaatagt gcaaaacaaa
     7441 gggtattctt agaggtatta tctctgtacg caaaaggcta caatcagtca caaattgtag
     7501 ataaggtcca catctcacag cctgtagtgt ctagaatgat aagcaagatt cacgacgaca
     7561 gcctagccaa actgccatac tatctgactt cacaaatccc ttgggaaatc gagagaagtt
     7621 taattaccta taatgaagtt gaagtcaagg cgtgggaact agccgaccat gcagatccta
     7681 gaattcgtca actcggtcta aatttggtaa tgcaatgcac gtctgaccgg caatcccttt
     7741 taagcacgct tcatggaacc aatgaggcta ttcaaactat acgagccgca gagatagcta
     7801 ggagagcaaa tgagataagc gaattaaggg agaatattat caagtcaacc cctcctgaac
     7861 tacagttaca aatcctacat taaaatgaag aagatttgaa agaagcagcg acctacggaa
     7921 taccgataga acagattcaa gcgagaaaga agaagagatt ggcattgata gaacatgaga
     7981 cgaatggaga agcattctag tgtcaataaa agcatatgga ctgaggttaa gccatcttag
     8041 tattatcaat catactacta tagatttcac cttaaaacga aggaatgcct tagccaattt
     8101 taacagtctg gatggtatat ttggagtggt catgtaggta atgactagac gttatcattt
     8161 aatagagtcg tctagtcggt tgtcgtctag acttgaaaag atccgtggca ttctcggtca
     8221 tgtcgtgtcg agagtgacac agacgccgcc cttggtaaaa tttagaggta tgggataaca
     8281 aatgaaacac agcttcgata aactgactgc aatcacccaa acatacacca gcaaaagtaa
     8341 acccctagac gagctgattt tacgactgaa gggaaaacca ttctattgta atagaccctt
     8401 taaatcagtt aacgagtact gctgcctctg gcacaccttt ccacctaaaa agccagacgg
     8461 ttcttatagt gagttatatc cctttcaaca aagtctagtc acagatatcc agacttacaa
     8521 atttctagct ttactaaagg ctcgaaattt aggggctacc cagatagcac tagcttatgg
     8581 actttacctc accttatgtc tacaattgcc gggaaattac atgtttatta caggagtcgg
     8641 atatattttg agccggacct tatgccgtcg tgctaaagct atgcttgcca caaagggcgt
     8701 ttatacagat gataacgtga gcactataac atttcctcat tgtagatgga tgttttatgg
     8761 atctgattct aagacatacc ttggtcaaag tgaagtctca tatttggtag cagatgaaat
     8821 cgcatcattc gacccctatt ctgactggaa aggcagtata gatacatttg ctattaagaa
     8881 tgccggagcc acaatatttt taattacaac cccagccggt agagttggtt caactgctca
     8941 tagagtattt acagaaacag atgataaatc actatatcat agagtttatc taagttttag
     9001 ggaagcgttg ggatcaatgc tatctccgga gtcaatccga cttttacaaa agacctcaga
     9061 atctttcgat atgatgtatg atctaaagtg gggatttgta aaacgtggta ctatgttatc
     9121 aagtaaatca ttagatgatg cattatcaaa acagtataat attgaagata ctacagtaaa
     9181 tattaacgct ccaaagtcaa tgggtataga tttgatctct tgcgtaatta aca
//
LOCUS       DAOR01000015            4863 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42617, whole genome shotgun sequence.
ACCESSION   DAOR01000015 DAOR01000000
VERSION     DAOR01000015.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42617"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1448 a          975 c          973 g         1467 t
ORIGIN      
        1 cccacattac cgtttcattg actatgccgg atgaatgcaa gatgagtccg tgtgtgctga
       61 atcaaattaa ttgatggaag gtaatcaaat aaacaacaag aggcgatttt aaaggatcat
      121 tcattactta tttcatgttt ggaaaaaatg aaacactgga tagggaatat tattggagag
      181 cgacaaagac atcggctatc cgcatagaat taatcgatca gaaaacaacc acactgaaag
      241 taggagaaaa gtcagagaac aagaatctgc catatcctga agcttcccga atcataatta
      301 caaggaatgc gcagaataat gatcttatct tagattacta ccaagatgac aatcgattag
      361 cccaggaaag aatatcagga cataccatcg caagggtatc tgttgaattt ccgttaccct
      421 ttaacatgct ccaacaaggc ttgacaatgt atgatctcat atatgcggcc gcatgaccaa
      481 caccggagga gggcatcctt tgactgcatg actttttgct atacatactc tacaattgca
      541 ttatttgctt atatagccag gctcggttgg tgttattggc cgtctttatt gcaaccggcg
      601 ccgcaatatt gggatatttt attctagccc agtcgattca ttcgaatgcg attatcgcac
      661 cgacgccaaa tcgagcatta ccgaccacaa tgtataattg tattactcat tgttctccgc
      721 agcaaaatgc atttatggac ggctacaaga tgggtgcaaa tcatgcaata tccggacaat
      781 tatatgccaa taattgcagt aaagacgccc acttctgttc tatatatcac aggggttatg
      841 ttacaggttg ggataaatac atctgggtca ccgaacatac ttgtcatcgg tgtgcgtgat
      901 gtgaggaatt ccattcgtgg gggcactatt gctggcggta cagtactagt cggcacgtta
      961 tcgactgata gagtgtagac atcggcatct ctgctgacta ttcgtatccg cccagtcgaa
     1021 gtggaagtca ttcaggcaag acagggcgtt ctggaaaaat tataagtgta ttcctcacag
     1081 tggaacgcga tgaagaaaga ccgtatcatc tcaaaaattg aagcctctca agatggaagc
     1141 cccttacgtg tatgttacat tttctgatcc aaatgattac aagccaggag gcgaaaagcc
     1201 gcaaaatcct tttggtccaa acttgatggc atttacttcc ccagaggaga tgatgaagaa
     1261 tttgccaaaa gccatgtcca atatttctag ggccatgggt ggaagcggcg caccaacgga
     1321 ttcgcccaca ttcgaaatca gtatgaggga gtacgaggat atggcaataa aagtgggcga
     1381 taaaattacc atagaaatta agaaatcaga tagcagcagt gggagataga tatcactatg
     1441 ctcagtcctt tctaattttt tatgctcact ttttccgggt cggattggcc tcttctctac
     1501 gtagatatga gcaacagcta taaatcggca gcaagaaggg ttagccattg ctaacactag
     1561 cggcgcgata aaaagaatta gcgattccgt atacaaagtg aaatcgcaga atggcaacgg
     1621 agactatgat gtttgctcga cggatttggg ctgggtctgc tcatgtcctg accataaatt
     1681 tcgcggtgta aaatgtaaac acattttcgg cgttgaaata tcagttgcgc tgcgtaagga
     1741 agtcgaagtg gcgcgcattg agtctatcga catgcaatgc tgcatttatt gcaaatcttc
     1801 gtggattgtt agagatggtc taagatataa caaatatggg gcgaaacaga aattcaattg
     1861 cagagaatgt aaccgatact ttacaatcaa tttgggattt gagaaaatgc atgctacgcc
     1921 tcaaatgatt acatcggcat tgcagcttta ctttacagga gaaatgatca gaaattcttg
     1981 acattgcaag gtgtgaatat gagtcacgtc gcgcaaatac tgtgattaca gatggagcag
     2041 cgaattttca tgatgttttt aaaaaatcca cagtcaacaa tccaagaatc agacatatat
     2101 cccatatccg tttacaaggc gaccacaata ataacaaaat ggagagactg aatggtcccg
     2161 cgcgatagag agaaagtaat gcgcggtctg aagctagcag acacagaaat tcttcccggg
     2221 tatcagatat atcataatta tttcaggcca catgaagcat tgaagggtaa gacgcctgct
     2281 gagaaatgtg gaatcataat tgagggtcaa gacaaatgga aaaccattat cgagaatgca
     2341 tcaaaggaaa ggtttaagct atcctagaat gttatgcact tcttaataca ctaatgtaga
     2401 tgcaatggct aagcagtaac gtctgataat aacctgcttc agcttcctca cgcgctcttt
     2461 ttccctggta gaatttatat catgaaaata tcgtattccg aggaagaaat aacatatgtt
     2521 gaattgagaa agcgtctaca aattgctgat ggtagcttgg tagctaattt tagatatctt
     2581 gagaaagcag ggtttaccgt atctcaaaaa caaattcgcg ggagaaagac ggtaacgaca
     2641 tatagtatta cagaaaaagg aaaacaggct tttcaggtat ttcgcgctag tatgctgaat
     2701 gtgttgtcat gattttatcg gaaccgaaac taaaataaat caattaattt ttggaattgc
     2761 accaacatgt tttgcgagtt tttttcaaat tggccgatta attcaggaca atccttgtct
     2821 ctgtatagcg gtccagtatt tggtatgaac caaatttgtg gttcaccgcc gaccgattga
     2881 tcgatttcat gttcagtcaa tagtttaatc ataaaatatc caacttttgc aaattgattc
     2941 attgtcatat ccttctcttt atcaaaaaat ggttttaaga acacgttact aaacatcccg
     3001 ctggagccta tccccctata gtcgtagaca tctgattgca ctttttcagt aatatgtgta
     3061 agcgcagctg ttttcgtgtc tcctatttgt gtagcaacca aaatttccaa gcttccgttt
     3121 gaatctcgag caactttact gttgttaatg gctttaacaa gaattccaaa attatcatgt
     3181 aatgaagcaa aattatactc gttactatac atatgtacaa tgcctgaggt ctggacagga
     3241 tgtttggcgt ataccagcgg ttgttctaaa cgaatatctt ctggtattat tgcgcctgat
     3301 tgcgtagcaa ataatgcgtt tgttctaaat cctttataca agtctgtaga acccgcgcct
     3361 cctgtgacta tatgatagaa ttcgctgtgt aatttcggtt catacgatgg aggatggtcc
     3421 tcatacgtca ctttactatt tgaaaccagt actaccccat caacacattt tccacctata
     3481 atgtatgtca attgaggatt tttctttaat cggcttttat atttaaattg aacagacgag
     3541 tacaccctga gacagcttta ttgtcatatt tcagtgttcg tccgacgacg gttaacactt
     3601 tagaacccag tcgatatgtt aacagaaccg aaacgaatga tagaatttat ttcagtagag
     3661 caatagtcta tcgctagaaa aatcgataag aagaaggttc ttccaaaggg ctttgatgtt
     3721 cacaggactc aatttgcgaa attagatgat ggatatttta ttattgagca aaagtggttt
     3781 ccaccaagcg ctaagaaatg acccgatggt taacaccctt gtcaatgacc cgttaaatta
     3841 acagagccca ttttagggct ctgtactcct cttcgtcgga tggcttattt ttggtagcct
     3901 gtatgcgttc gtcggataga attttaccag accttttgaa gaataacatc gccttatcaa
     3961 agtcaggaag agggattcga ttcttcttac aatactccaa taaccaagcg atctgtgttc
     4021 taagataatc tagcatttca taattcattg attgtaattc ttcaacccta tccatggcat
     4081 ccgataattc catgacttat catgtgactc ctggcagatt cttctctgca ttctgttgcc
     4141 gcttctcgtc ctgctttttg atttcttcag attttttctg tattaagcgc tcgaagcctt
     4201 cttgtaagct tcgagtcttc tcttcccttt cttcaatctt cctgaatctc tcttcgaatg
     4261 acctcgtttg catcgtttta cgtgaaatat cagatacagc gactcttaga gctgtgatat
     4321 tggcatttag aagatctatc tcagcttgtc gtttctcttg tctctcggct aagatctccg
     4381 ttctatatga taagtcgtcg ctcacactat caattgcagc taatacggat ttagacactt
     4441 cgagcgcgag ggccatttga gggggtgatt tttccagact atctatcctt cctcgaagcc
     4501 aatccctata ctgctttcgg agcgcactaa tctcgataaa atattctttc agggtctttt
     4561 tcatttcctt tcacccttta cttttttgtg agcctcatcc tctatccact tctctatagc
     4621 ttgttctact aattcagata attccagatc attacgtatt gcttcaatct ttgcttgctt
     4681 ccaaagctgt ggatctattt tgatagatgt cgcttccctc ttaatgtcat tcctcggtgt
     4741 cattattata ctactatacc taccgtagta taaatagcta gtggtaaaga tttaaataag
     4801 catagtgcta taacactagt agtatagtag tatgagcaaa acagctataa accgtcaaca
     4861 aga
//
LOCUS       DAOR01000016           17398 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_44006, whole genome shotgun sequence.
ACCESSION   DAOR01000016 DAOR01000000
VERSION     DAOR01000016.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 17398)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17398)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17398
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_44006"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    187..209
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5299 a         3380 c         3239 g         5457 t
ORIGIN      
        1 gctcggagat gtgtataaga gacaggcacc agactataca accgaggcag aaaggaatgc
       61 tgtttttttc tttggcaaga aaattcgaga ctgcatagtc gtggttattt tttgtctttt
      121 tacgtggata ataataattg tcattaatac caatggatgg cattggtgtg agcatcgaat
      181 tgtagcnnnn nnnnnnnnnn nnnnnnnnnc atgatattct ctcctaaaat tggtttgcat
      241 gagaacgttg tagtactaga ttatgaaaac gaatatgcaa atctaattct caaacataat
      301 ctgagctatg aaacggtcac atcaacggaa gacggaagaa ttatcgggaa aagcaaggac
      361 aacccaggcc tacttccaac cgtactagaa agcgttctca agagacgaat atttttcaag
      421 aatttacaga aaacgtttct cattaatacc aatgaatggc tttggtgtga gcatcgaatt
      481 gtagcactaa aagatatcct tgtctatctg ataaaacagt actacttatt cctgggagcc
      541 ctttttaatt ggttgaaacg aatggcttct ttattacaaa gattctttag aattaataat
      601 taccatcgtc ttcctagaac ttcccaaata ttggatatct cacattcctg agtttgttgt
      661 actcttttag gttgcttcat aatacctcca catagttctc actttcaccg atttcacgaa
      721 gtgcaggtgc gatacgcgta cctattaatt cggtagctcg catgagcttc gcgtgaggta
      781 atgaggcggg gttcatcata aaagtgatgc gggatatgcc gcctagcgct tggctgtgcc
      841 ggatgatctt ctcgactacc tcatttggat cgcctacgag aagtgcacct tgcagacctc
      901 gctgggcttc aaaacccgca cgcgtcatct taggccatcc gcgctccttc ccaacctcgg
      961 tcatggtatg tgcatagcct ggaaagaagt catcgactgc ctcttgtgta ctctctgcga
     1021 catagcccag cgaatgtacg cctactttca attgatcgga tgaatggccg gctcgccttc
     1081 cagcctccct gtaaagatca atgagtggcc ggaatctgtg cgtttcgccg cctatgattg
     1141 cgaccatcaa aggcagtcca agcaaaccag cacggacgaa tgattctgga gtcccaccaa
     1201 cgcctctcca tataggcaaa ggattctgta aaggcctcgg atagactccc tgtccattca
     1261 gagtaggccg atacttgcca gaccaaaaga tatgctcatt gtcacgaatc ttcagcagca
     1321 aatcgagctt ctcttcgaaa agcgagtcat aatcttccaa tcgaaatccg aacaggggaa
     1381 acgcctcgat ggatgagcca cggcccacaa ccatctccgc ccgaccttgt gacaaaagat
     1441 ccaaggttgc gaattcttga aacacccgta ctgggtcggc tgcactcagt actgttacag
     1501 cactcgtaag acgtatgcgt ttcgttcgcg cagctgccgc acctaggatg acagcaggag
     1561 ccgaatcgag gaactcctga cggtgatgtt cacctattcc aaatacgtcc aacccaattt
     1621 gatcggcgta ttcaatttgt tcgatcaatc ttcgcaaacg ctcagatggg ctgactgaga
     1681 ggccagcgtt atcaaacgct gcgaagctat caattcctac ttgcatttaa tatgcattta
     1741 ggtaaatgat tatttaagta tagtagtaat tcataactta gtaggtgtgc acaaccatat
     1801 ttagtaagca gaaatttact taggtgtact aatgatacgg ttactgtgaa tgtgcaaagg
     1861 agcactacga acactaccaa ctcttcgact aatcctgcca ataatactca gcagcgaata
     1921 aaacctgcaa gcataggttc acaaccacca actactacaa agatttctga gagagggata
     1981 tatcaagtgc aactgacttt tatctcaaca ttacctatac aatctcctaa tcttcttcct
     2041 aaaagtggat ttcagatgga aatagatttc ttaaacgcaa gtgctccagc tccaacgatt
     2101 ccacaaaaag aatcagctat cagaggagag tcaagtctgg gtatgccagc cagccaacca
     2161 aatatcatac aacgattagt accagtagat agttttgata tgaccgttta ttccaacagt
     2221 ggcaaagttc tgtggaacaa gacaaatcag gcagtaaccg ctgggagagc tattgagact
     2281 atctcatttg atggtaacca tactggccct attacaattt taataaataa tatcaaatct
     2341 agcattgtaa tgacatgaag tgtcacaaca cctggcatat ggaaactaca tttaaaaaaa
     2401 agtctatgcg ttcagctgta ttaagtgtag gcatgatgtt tattgcaagt tttgcttcgc
     2461 ttacacatcc tggtttggct aacgctcagc agaataaaac attaatgcaa cctcatggag
     2521 tacagagtag acaacaacga atgcctcaaa gatgaatatt attctagtcc atggggcatg
     2581 ggctgacgca tcttcatgga gtaaagtaat tcctattttg cagcacactc ctaggagggg
     2641 gccattatga ctgaagtcgg aactagagac atccagccgc tgaactggga cgtgctcgta
     2701 acgccaggca tacccattgc cacgagcgac ctggctccgg gcatgaaaca ggcgatgttt
     2761 caggcgatag cgtcaaccct catctatggc aagcgagaca ccgttctagt cgacgccttt
     2821 atgaccgtca agcaggctga tgccctagtg gattgggtag cggcaagcgg caagaacctg
     2881 acaacaatct atattacgca cggccatgga gaccattggt ttggaatcgg agctcttctt
     2941 gaacgcttcc caaatgctag agcagtggcg agacctgatg ttgtgagagt tatgcgccaa
     3001 caggcctccc cagagttcct tgagaggttt tggaaggcgg gataggtgtc actccattta
     3061 gaagtattat aaaatacgcc acaaataaac aattgcctgt aaagataacc atgtttgact
     3121 ctaatcgaaa tgaagagaac atactttaca aaaaagaatt tgatgaatgt gcagatttag
     3181 acgcaaattt gaagattgtc tatactctag atgtaagcga taatgattgg gagaacaagg
     3241 ctacataaac caagcgatgg taaccaaata tttgaactct aacgaactag atgattcgat
     3301 atattatatc tgcggtcctc ctgtcatgct aaaggccatg caaaaattgt tacaggacga
     3361 tttacatatt ccaaagcaaa gaatacagat tgaagaattt accggctact aaataaatgt
     3421 gacaacaacg ttgtggtgag atattttgga tatgaagagc tgctaacaaa gattggtgcg
     3481 aaccaagaca gacttaacaa gcttgtgatt gacctatcaa catttaatga tctgtattca
     3541 attctacgag aagatgttct tgctagtaat cgccttgcta taaaacccgg tactgaagga
     3601 tattggcaac gagtcttctc tgccgctcgg aaggtagctc atcacattta cgatacaccg
     3661 gactgtaaac atccgctaat atatgaagac gataccaata aagataaaaa atggattact
     3721 tcagccgatt tggaatacaa gagactgata atgatttatg ctttggtaag attggcgtgg
     3781 gaaaagaatg tattgataat aggattgatc aaggatacag ccgcagctga actgacgaag
     3841 atgatagttc caatcttaca aaatgctcag aagttcaata tcttaggtgt ggaaggagcg
     3901 gcaggaaagt tgcccaactt caataccgac acgcagcttt tgcagacatc tagtgcaatc
     3961 aatggagaat ttgttatcaa tccatggaga acctttgagt ttgattcatg cttcagaaca
     4021 atagctccta tagtagacaa tgaggctgct tcaaaaaata atcaatcaag agtaagaggt
     4081 gctttcaaaa atgttatttc ggcggagaga atattcgtca aattttacat acaactttgg
     4141 caaagcgaaa atggtcatat tgaactactc tctgaacgtt tgcttccaat cttgacagaa
     4201 agtgactagc tcgcaccact catcatagta cgtatgtatt tatttagttg actatggatg
     4261 tgttaatcct tttcgctagc cgctcttctc cttaaaacga cctttcatga ccatttgttc
     4321 taatggcttt ctatcactcg gcttctccat ttcctgaaat ttcgaaggaa gcatgtcttt
     4381 accacctctt tttccgattg cgaccattgc aataatatcg aagctatctg gaatgtcaag
     4441 atcttttctc gtccgcccat agtcaaatcc tgctatcccg tgaacaacta aacctcttga
     4501 tgttgcttct aatgcgagat tctcccatgc agccccagta tcaaattgat gtgttactga
     4561 tggaatgacc tttccattgt attcaaattc tttttttgag actatgacaa tcaatacggc
     4621 agcattttta gcccagattt tgttaaagtc taccagtaag ttgaaaagtg tattccactc
     4681 ttctgtgtct ctagttgcat aaataaatct ccaaggttga gaattaccag aagatggtgc
     4741 ccatcttgca gcttcaaaca ggctcattaa ttcttcatag ctcaacttct catcagtcat
     4801 tgatcttgga gaccatctat tgagaatgat gggatttatt ttatagattg agcttctaac
     4861 ttctctcgga gttgattgta acaattgact accaattact ttttctacca ttaccgtatt
     4921 aacgagcagg aattatttaa gaatagtagt aataacatac taagtaggta agcaatatca
     4981 aactggattt ggctgagtta aaaaatgact ccggtagtat taatattact aactaagtaa
     5041 gatgcaacta ctaatcttaa atatatggca aattattatg atctgacatg gttcaaaatg
     5101 actttgttga agttgcaaaa gtaaatgaaa ttcctgctgg gaaaatgaag catattgaag
     5161 ttaatagcaa agaaataatg cttgcaaact tagaaggatt caaaaattga tatcaatctt
     5221 ggtaaggcat ctactgctgt ttttctaata gaaaaatcca tttgataact aaatggcgta
     5281 ttttctggat ttatttcaac tatcatggct ttattatttt tagcataaga atacaatgta
     5341 ttagccggcg atacgacaag tgaagtaccc actattatca tgacatcaca agctacagaa
     5401 agcattactg ctccgttcca tacttcttgt tttatgcctt caccaaacca tacaacatca
     5461 gggcgtaagt tacttccaca tatcttacaa ctaggtggga gatcagagaa ttcacttctt
     5521 atcttgcctc tgaaatcaca tttagtacat ttagttgcaa atatgtttcc atgaagctct
     5581 gtcacctccc tgcttcctgc catctgatgt aagccatcga tattctgagt aattactgag
     5641 acttcgctgt ggttctgcag atttgctatg gccagatgtc ccacgtttgg ttttgcagcc
     5701 attattttcc ttcgtctgtc attataccaa ctccatacta atttcggatt atctctgaaa
     5761 gcttgagccg aagcaagctt cattggatcg tattttttcc acaagccgtc ggttcctcta
     5821 aaagtactaa ttccactttc ttgtgatatc ccagccccag tgagaaagac gacttccttg
     5881 gctcttttca gtgtatcttc aaaagtatca atcatcgcat tatttctact ttatcaaaag
     5941 ccgtatttat cttgccgtgt cttgatactt caacaaattg accatcaaac taacaataga
     6001 tgcaaacaag caaatccaga gtatcataaa aaaatacaag ggaaaggctg acccgatacg
     6061 gcgaaaggat gcgattatgt cctgggagcg aggacgttga atgttatgcg cagctcatgg
     6121 ctaattgttt atgtcggata gcaccgtatt attctcttga aaactttgtt gtgcaaagga
     6181 gtatttcatg tgagggacac ggagtaataa tgggtgcaat tcaataaaga taaccaaaaa
     6241 caatcctacc tatttcctgt aggtttgaat aggttaatat ccattatatt tgagagtaat
     6301 ttaatcaagt tcaaagcaat cttctgtagc acgtatacta ttaaagctgt aaagcgctat
     6361 gaagattctc cacatacaga aggtgcataa tctatgaaat cgtattctga ctggtgttta
     6421 agtgctatac ttatattgtt taattgtcgt tatctgaatt agaatcattg gacactcatc
     6481 aactaatgcg gtatacaaac atgattcttg agaaatcagg actaccatct aattccatta
     6541 tagcttcagt aaagcgtttg agcagcatca atattgagaa tatttttacc tataaacccc
     6601 tgtctgaagt agatgagatc tgccttagat tgaatacttt aaaaaagaaa tcaactgatg
     6661 cattcagacc cttcatctct aattcgacaa agacgttgtg tcattcaatg ggcaatcctt
     6721 tgagagtagg agaagatata attgaacttg aagaaagaaa tggtctcctg aattcactat
     6781 taagaatagt tacatttctt agtaataaat atgccatgga ggcatcaaat gcagcgctga
     6841 taggagagtg tcagtaccca gaaacgtcct ttgtctttga tgaacaaaat tcctcttcct
     6901 acagaaaaga aattgcacat ataactaggt attatagatt gcatctagga agtttcctag
     6961 ctatcgagct tgcaaaagag ctcaaaaact ttccattagg ttacaaagac aatagattcg
     7021 aaagtgtaat agaactatat gaaattggat gtgctgacta tttatttaat tttgtagcgg
     7081 atacaaatac aaatatcaga gaagagaagc ttgtgacacc attcctaata gatttggatg
     7141 gcaataggaa agttcttgga atccacgttt atggtgatga aaaaataaga ttatggaaaa
     7201 gatggggaga tgattatgac agactttatg acattagtgg aaacagaagt aatacgcgca
     7261 tggaagtaag tccattctta aattcggttg caattacata acatgcggat cctaataatt
     7321 aacaacctgc actatgatta gtcgagatta ttgttaagtt ggtaacacaa aagtaaatcg
     7381 gttgctgcta tgagaacccg aacctcttct atcaactatc cacctggtcc cacccagaag
     7441 ttacctttgg ttttatttct taatttctta agagatccta tccgagtgct gattgatact
     7501 tcaaaatatg gagacatttc tcattttaaa tttggaaacc aacatatcta tttcctcaac
     7561 catccagact acatcagaga cgtactagtg actcataatg ccaacttcat aaaaagccgc
     7621 ggattacaat tggcaaaaaa agtattggga gaaggattgc taacaagtga agggaacctt
     7681 catcgtaacc agcggcagtt aatacaacca gcttttcatc aagaccggtt aacagcttat
     7741 gtggccatta tgacagattg tatcttgcgt atgtctaatc gttggaggga caatgataca
     7801 ttggatgtac ataaagaatt tatgcaactg acgctggctg ttgtttgtaa ggctttattt
     7861 aattttgata tcgaatcaga agcaaaagag attggtaagc atgtcaccac ccttgtagaa
     7921 tatttcaacc gtgctagaat accgttagct gagcttatcg aaaagttacc tttaccaagt
     7981 aatagacgct ttcagtatgc taagaagcag ctagatacaa taatttatcg tatgattgat
     8041 ggcaccagca ttgctaaggg aaacgatttg cgtcagcatg atcgtcatgg tgatctaata
     8101 tctatattgc ttgcacacaa gaacgttaac tatgacaatg ggagctacgt tatctcaaat
     8161 ttgcagttaa gggacaatat tatgacgatt tttttggctg gacacgagac tgtagccaat
     8221 gcattaacat ggacatttta ccttctctcc caaaacaaga gagaagaagg gaagctccaa
     8281 gatgaagttg actcgattct tgacaacgat ggagttccga cggctgcaga catccctaag
     8341 ttagaataca ccgaaaaagt ctttgcagaa tctatgcgac tttatcctcc ggcttgggca
     8401 gtagggcgtc aagcaataca cgattacagg atcggagaat acactatacc tgctggctcg
     8461 actcttctaa tgagccaata tttgatgcat cgcgatccac gatttttccc cggacctgaa
     8521 agatttaacc ctgaccgttg ggatagtgag gcaaaaacca acttgccaag gtttagctac
     8581 tttccatttg gtggcggtcc tagggcttgt attggagagc catttgcatg gactgagggt
     8641 atactatctg tcgctactat tgctagaaga tggaagatga aactagaacc aggccatccg
     8701 attgtactcc ggccacttgt cacattgcgt cccaaatatg gtatgcggat gaagttaata
     8761 agaaggaatg tgtagggtgg gtgaaattag aaacatagtg cctgttggaa cgtatagtac
     8821 gagcattagc catccatgat ggaaaggata aggctaagcc taagtgttat ccatcatgta
     8881 aaacttgaat gtgagagata cctacagtaa acgatatgtc agactattat gataatagat
     8941 gggtgatcga caggctgtat atgactatga cttaagatga tagatcaagt aagaatctat
     9001 tcgttcatat cactagtcca ggagaaggag cgagggaatt aaattatggt tcctcttcct
     9061 ccactctctt aaaacgatcc caacatgtaa tcgtgttcct atatcgacta ccagaagatt
     9121 caatgtgtat atctacataa acatataaac agcaatcaga taaaggcagt catgtatttc
     9181 aagaaacctg tgctaacagt catacacaag ttctttattg gatatgaaga aactctcaat
     9241 acggaatttg gtacacgtga agctgtcatg gatttagtcg acgccaagga atggattaga
     9301 ctttataggt ctagtactaa aaagattgct tacaagttct ggcttgatac ctacaaacgt
     9361 ctacagccgt gtatagtatg cgatgaacga gatccagaca aattgagttt acatcacttg
     9421 ggtgaagaat acactcgtag acaaaagaaa gatagaatcg ctaatatggt atctaaccag
     9481 ctaccactac atgaaattga acatgaaatg atcaaatgtg caatggtgtg tttcaattgt
     9541 catcaaaaat atcataaagc aggtaagcaa ggattggacg acttgtatga tctcagcgag
     9601 agagtttatg gcatatacta tgggcccgga gccgatgtag aagaaacaaa acatcttcgt
     9661 agaaacttca ggaaagagtt gaataacgtt gatatagaat tagtcgatga gaacaggagc
     9721 aaaaccgatc aagagcaaga tgaaagaata gccgtatcta tatatcaagc aggccgtcac
     9781 aaatgccaag acgaggatgt gatgtatcta tattcgaaat tgtggacaga aagacccaca
     9841 ttacatgtta actttaaacg ttagtctttg acctttttat actaattgac gaacagtacg
     9901 gatagtaagg gatcagttga atgatcaaaa tttgatatgt ttagccctaa ctagacttgc
     9961 ttctagtgac cttatatcaa agtcatagta tttggttggc tttctcgatt tggatttact
    10021 tttaggtttc ggtctggata gatattgaaa tgaaagattc ctatgcaaca acgcagactt
    10081 aactattgcc ttttcgaaat caaggaacaa gtctccgggg cagagtgcca gccctaaatc
    10141 accgtcacta tcaatagtat aggccagatg tcttggcgat cttgacttag cgatgacatt
    10201 cagatatcga tcacgttctg caccacttaa ggaaacgtaa aagtgttttt tattagtgag
    10261 atcaactatt atctgttgat attaatggcc aagaccgttt ctaataaggt cgaaaaacgc
    10321 acaaagcaat gaatcacctt tattattaac aatataagga agatcaaact cggtagttag
    10381 cccgcaaggt ccagggagta aagtaaagta ttttggatca attctgttta tctcattaga
    10441 aaaattattc aataacttgg aatcattatc acaaagtctt gacgcgaact caagcagatt
    10501 cattgcttga aaggcaaaca tgaaatttcc tcttcctatg ctttgattgt aattagcagc
    10561 aatagaatcc caagcacctc taaagtcact cacaataaat tggtaaatct catttgggtt
    10621 taggttttga accataacaa ttgatgagga tattatcagt ctaatataat cagcgtcagt
    10681 tacttgacgc tactttatcc tacttaccaa atacagtaaa ataatgaaag tgaaggtgtg
    10741 aacgcaatga agactgtaat gggtgcttca caggaactgc taaaaatata agagaattag
    10801 tcgacatatc gaacctctaa tatattcatt agaaacatca cagtctaaag cgtttgctca
    10861 tatattgaag agacactcct ctactgtctc tgtctgtcaa agaattatta tgaatggtat
    10921 gtaggatttg gttagctaca aacaaatcat catatcctat atgctaattc tttgtaagaa
    10981 agaatattag tggacgctaa actagctttg ctaattacaa gagtacaatt caatgcaaaa
    11041 tgataggatg taaggaatga aagcagtgag gtgtttatgg ggattaacac atattaataa
    11101 tcatataaag ataataccac cgtttacttt ctcatgcacc aattcttcca caattagcag
    11161 tgtttggaaa taatttctat gtagcatgga agtcacaatc actcaggaat atcgaatatc
    11221 tggcaggtag tgaccgaagc tgtaacctat ttttgaaaat ctttgcaatc agccgaggca
    11281 taaaaaatct cattggccaa actgtcaatt gatttgtaag tatcattcct tctcactaac
    11341 ctcatatttg aggatacagg tctaatactc ttatcatccg taagtgttgg atatatttta
    11401 tctgctctgt atgttagttc ctttgtacta ttattcattg ggtttatttc atctcctctg
    11461 tatgttaatt ccctcgtccc actattcagg gttgataaat tgtattcttt cttctttatt
    11521 tcattttcaa gccagcttac tccatatgtt agattagcta ctttcttttg taaatatggt
    11581 aattgcccgg tattgattaa agttagcaca ttaattatat catttttatt catacctcga
    11641 gattcaacta ttctaaataa ttccagaaat gacgggagat aattttctat ttcatcatac
    11701 acctcaacta gtttatgcat attctttaat cccaaaaact ctcggtacat tattcgagcc
    11761 tcctctggag gcagatctag ggcaattact acctctacaa gatttttgcc ttctgaaaat
    11821 aacttgatgg cctgagtagt tttcgatttt gatttattat cataatcaac tttcccctct
    11881 agtttccccg attctccttc tatctcagcc ttcactttgt ttgttatgac acctatatcg
    11941 cgaggggaca tgtgcacccg ttgggcaatc tctctgacag tcttatcctg tttgtaaagt
    12001 tctattacat attgtacttt ttgaactcga gtcatattgg atatgaactc cattatacat
    12061 tataatatac gaacttattg atagctttat cggatattct ctgtttcctg ctgttaccag
    12121 tttgttgcct actgttgtct atgtgttttc atctgttttc ggctgctacc ctctgttacc
    12181 ggaaggatcg taccgtgcga gatcgaccca ccccatttgt actttagaaa aataaattaa
    12241 atctgaaaaa tcacgtcaaa actacgttaa gtccaatagt ttgaatcaga tgggatccag
    12301 ttttctacct tagaaatact aggcctgaac gatcttgctg aaataaatcc ggctcttatc
    12361 catggatttg tctttgatag ttccataagt aatgaatgga caacttgtct gtgttctagt
    12421 ggtattggtg cattcaatac tgcatccttt tctcgacatg ctttagccat cataatacaa
    12481 ccgtaccaca taaaaataga tttcatgatt gtatcttcga ttgagttaaa atttgcgtgg
    12541 tcaaaatttt gctgaagaac tttttcccca ccgatgtcaa gtgatgagtt agatgatcac
    12601 cggtcgtgtt tcgtattccg acggcagatt tagccataac ttcgttttcc actaactcga
    12661 ctatggttat tacttgcgac tttcctatca cacaattatt tcctagaact aaattgcaaa
    12721 cacttctaat agtctcagca atatgcaata gatctgcggg gttagaatct ttggccttaa
    12781 ggtgttcagg ctgaaaaaat tgccacgctt gtgatgcctt tagtactgca tccttaacac
    12841 aggacatgac taggttctat tacgtgtgtt ataatcttga agatgtttca tcagtacttg
    12901 cgagcaccca ttgaatcata cccaatggat cgtttgtgct ttattagcat atacaataat
    12961 tgaacgagtg tagagaatgt agtggtaata atggataaaa ggcaaccatc acgaacctta
    13021 caatatcgaa ccgtgtagga taatactaga cctcatggca catcgtgtgg ttcaataatt
    13081 gtgatcttaa tccctccata ttgaatccag tgatttccaa catatcgctt aatttattca
    13141 acaaaagcct tctatatttc tctaagttta gattatcagg ttttatagaa taagtcctgt
    13201 tacttttctc atgatgtaat ttaggtaggg tctcatacca gcaaacagtg tcgcctttct
    13261 gcgctcctaa catctttgct aaaaccctca ttctatcatt ttcgttcttg tactcttctg
    13321 gttctttgga aagttttgtg acaaatgcca aatcttgatg gttaactttt cctgatttca
    13381 gttcgttgaa cgcttgttct atgagcgatc taatcgcatg atagtcttca gatgttatga
    13441 tgtgagttgc tatctttgct atattttgtt ttatccattt aggatatcta ccactcattc
    13501 catctaagtt ctttaaaata ggtttatcag ttggattgcc tgtccatgca acatatctat
    13561 ttttcttatc aaaaattatt gtatttatga atcggttctt gtgttctagt gtaatattat
    13621 atttattttt acattctaca atcaaactat ttattgtttc tatgcgggca tttttgatga
    13681 atattgaatc tgtgaaacca aatatggatt ccaagccata tttttctgat gctatctttt
    13741 gtagttgctt gtgtatcaat ctaccatatc cagtaatcaa ttcagatact ctataatccg
    13801 aaaaatcaaa ttcttctctg ccaaatacac cgtaaccagc attagcaagt aattttaagg
    13861 ctaagagaca ggcattatat tgaacgattt tttgcaaatc tctcttctct ctaggtttgt
    13921 tttcctcttc ttttagcaac gtctgatact tgtctctctc acgaatcaaa ccttgaagtt
    13981 tagttggaaa tgctcccttt atacgatgac atatccaata tggtcttgtt ctagcctcct
    14041 tatttaattc cagaaggctg ttatctatct catccatgac ttccgctcca acttgtgatt
    14101 ctcggtcatt ttgacaacat tgacaattta cagtttcgaa tgatatgtta tggtcaattg
    14161 ctatagtagg atacatccct ttgacatcca attcatcgac aggttgattt ttgtaaaatc
    14221 cctttttagg ttgaatacta tttccacccg caatgtattg tttaggtatc tttttgtttt
    14281 gggtatattc aaagacacat tctctcgttt ttatcatatt tttgtagata ttagcatacc
    14341 attgtgagac gccggtatga cagcaccgat aataatccat ttcagagtaa aaggcaatat
    14401 acttcatgat ttccaaagct aaacaatcat tatcataggc aagcatcatt gtcaactctg
    14461 cgtctcttgt aacatatctc atctgtatat caaatggtag gtcatttgca ttttctccac
    14521 taactatgat tttgctgcca gttacaggat ctttgtatgt atattttcca attcccaata
    14581 aactttgagc aacatcttct agcttatcag ttctgtatct tttgttaaaa acaccatttt
    14641 ttataatctc tttgctgtag attttataga ggtctatgtg ttttttattt gagtctacca
    14701 atcgtgcata tgtcgctttt acttttatag gagagtttag atgatagaga atacaccttt
    14761 gatgcaaaac aaaaaagtca gaatcatggc cttttactct attaagacct gtatcgtcaa
    14821 atactgctac gccagtggta taccaaccaa aagttaatgg aaactgattc aaataaaata
    14881 agatatcctg tattaatgct ttctcaggat taggactagc agaatatctt gagatgtgca
    14941 atacaaccct ttcaccccag ttagaacaaa agcacgctgc gaagatcttg gtcttcttat
    15001 gttcatactt tcttttgtat ggaatccatt caaggtcaat acttgctgag tctttaactt
    15061 ctggcctatc actttcgatc ttatctagtc gtttcttcga aacagctaat gctgataaag
    15121 tcatactgat tcacctccac tgttcccact gctactgcta ctgttattct cgatgcctct
    15181 atcctcaatg tcttctaggt cattattagt tatcacatgc gcggctgatg agtgtcttaa
    15241 cgttaatctt cctctttctt tattatcagt aattatgtca gaaataatgg aattatccat
    15301 ttggtattta ttggataatt ttttatattg ttctttgtgg aaaatcccgg aatacatcat
    15361 agtcccgaat attttgctat aataattacg gaaaagaggc tttacaaaat atccatccaa
    15421 cctaaacttt ttctcatagt cttttacgaa tttgcatata caaatttgtt cgttttgttc
    15481 gtcatagagt tcaaatttgt ccagcttaac tggatatttt aaaagatcga aaatctcaag
    15541 ttctaaccag ttattgggaa tatttctaca ttctttaggc aatataactc tgggatgttg
    15601 taaaatatta tccattcggt cggaaatgga taatttgttt attttttcca tcgatcctgt
    15661 tctatcaggg ttatcaatat tttcttcatc ttgaaaaact atcaggggaa agtatagttt
    15721 ttgtctccta tctatgacag aatcttcttc atcgactaac ccgttattaa tatactcgtt
    15781 aacgtaattt tgcttcatat tatttgtagt catagttttt cctgtttttt ctttgtaata
    15841 gtcacacact ttttttgttg ttagtccaat gatcctttcc tcttttgagc catcagggct
    15901 tttatcagga accttctttg aactgtattt ttcaagaaaa acttccttga agatctttac
    15961 tttatacggt ggcaccccgc ttagattttg tgttatatga aatgcttcat ggagatcttc
    16021 ttttatatct gcgataacta gagtctcttt atcgtattct aatctagccc tcagatgcgc
    16081 tcgcgccaat gatatgatac taagaaggga gagtattctt ttcgttattc tgttgtcaat
    16141 tcccttctct gcgggcagaa tttctgctag tatctgagca tatggaatcc aaacaggatt
    16201 aatattatta ctattatgat tgatagtgtt tttctcaatg tgttgactta tttgataaag
    16261 aatatgtgaa acacatttct tcgctaattc gacttgatta tttgatacga tttattgact
    16321 gttgcaataa attaggcaac cccatctttc gggctatcag cctatttcca tcgagataca
    16381 tttcctgcac catattcgga gaagtaataa gaaacctgct ttgtatttca ggccatacag
    16441 gccagtgtga ctcgtttctt gcactacaaa atatacaagc gggccttttg accggattgg
    16501 acaggggtat aaacgatttt ggaatattct ttctattatc gggcgtactt caattatttt
    16561 gggtaattcc catgataaga cgttggggaa gaccatggta ttactctggt cttggaggaa
    16621 cgatagtgtt gatcatcatt tgggttatca cacgggttcc aaatccgatt acaaacggaa
    16681 tagcattacc aataaattct ctgagtatcg tgacagagct atttgaaatt gctttcatca
    16741 taataacagc aataataata tcaaaagaaa gaagtaaaag agcacctgaa attaaacaag
    16801 acgtacgata ggaaaaacag caatttagaa atcttttccg gccgcagact agatttgagt
    16861 gtttttgtcg ggaacaattt gaaaccagtc gactaatctt ctttctggaa aaaacgtatg
    16921 ccgtttacac atggcagcgg gaatattgaa tccgtcataa aacgtatata tctatacagg
    16981 caattgaata cagcctataa ttgaaaaatg agaaccgttt ttttgctaca atcattgctc
    17041 ttgcagtcac ttctttcgtt ccggacacgt tcgcccagaa tagcacatta ggaaatgtga
    17101 ctacgccttc cggaattgac tggagcatac tatgcaacaa cataagccaa atgaacatac
    17161 tatttcagtc atgtgataat cttgtgagcc aaaatggtac tttaacatct gcaggacaga
    17221 tagcaatggg atgtattaaa agtgcattgt cattgggatt ggaggctcta catcatggag
    17281 taccattatc gagagtcata ttcggcctta atctgcttac acaaccgtcg ggatgtggcg
    17341 gtcaagtaaa catgaacgcg atagagtctg ccaatcaatt ccaatttctc acacgcgc
//
LOCUS       DAOR01000017            3311 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_28284, whole genome shotgun sequence.
ACCESSION   DAOR01000017 DAOR01000000
VERSION     DAOR01000017.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_28284"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          937 a          684 c          580 g         1110 t
ORIGIN      
        1 aagggataaa ataaatttgt ctgatgtaga ctacaacata gaagcaggca aggattatct
       61 agaaggcaca tctaagttta aacctgtaaa ggtcgaactc aatgtttcaa acgcaaagac
      121 agaccaagtt cattactgga tcaacgaaat tacaaatcaa tatgcggatt tcgttgtgac
      181 aaatgtcaag gccgatccta ataaaatatc ctttgaaata ggctcaccta gtatggagga
      241 cttgacagct gaagatattg agtttagaat gaaagaatac cttgatatga atgtaccacc
      301 ttttgaagtg aaggaattaa aggtacagta gaagaagtat ataagacaca agcaccattt
      361 ttttataaat tgaaaacact acgataagta acacctacta tatcaagtct ttcgtaagcg
      421 accttagcct ttatgtacat agggcctatt tttgaagctg gaatattgta atattttccg
      481 ttgaattcta taacatcctc agtccagatc cgttttaaca aactcatctg ctattgcgcc
      541 tttatgctca tggaacaata gaaacttcgt actcctcttt cgatcaacca atcccaagac
      601 cgcatatagc tcttcccagc gatagaacgt caagcgttgc aaatcttgtt gcgaatatta
      661 gagggttatg aaacaacata tctattacag atagcagata atgaagctat aacgtactat
      721 ctcttttttc ttgtaagtat tccttgaagg cttcaattgc aaccgcagtc aaagtagcgg
      781 cagccatatg acagttcgaa caattcgact gattaattag accaagaccg tcgtttgttg
      841 tagaaatttg gaccacaaac aggacacgat actttggaag attttcaacc taagcccata
      901 tcgctaatgc taaatacttc tcggttcctt catgctctga aggtttaggc cttctggtgg
      961 caaattgaaa ttgaaatatc tgtttcaaca gaccagattt atttgaatcc aactatggat
     1021 tctaactatc gacctccaat aggtttaaaa tctaccagta ctttttgtgg tgtcgcatga
     1081 ggtttcatcc acatacataa ggttacgtcc tatagctaga tctgaagtat acgactgtag
     1141 acattgtcat atttatattt atattcctaa acttgtctta aaaattatct cacatatcca
     1201 actagatatg ttctctcctc taatttcata ctctgttttc aagtccttcc ttgttacttg
     1261 tttgtctttg accacagaat ggtggtggat gatgataata atggtgatgg cttccaacgt
     1321 gactttctgg aaatttagtc tctggaattc ttttgtgata gtagtttcac cactaatatc
     1381 ggttctatcc atgcgttctt tatccacata tgcatgaacg attcttttta gatccttgcg
     1441 tgctaccaca aatcgaagcg ggttgtataa agcaataggc caatgattat cctcgagctc
     1501 gagtcttaca ccttcgcata gctggttcag tatctcgtgt ttgtcatcct gagtagaaat
     1561 tgactccata tcttttgcta tttcgattgt cacgagtttt gtttccattt gattcagctc
     1621 gtggaccctc tttcccgatg gcggcatacg ttaagatcta tccctctagt ctccaacgaa
     1681 gtcctgtgcc gtaggcttcg gcatctccat ctattggatc aattctttca ttgattttgt
     1741 cgaatgctgt ccagcctcgt tagtttttaa tgtatcgtaa aaaaatttca ctagaagtaa
     1801 gttcgtttaa gcatgtttcg taggcagcca gatacgaatc ttttaatatg ttcttgggtt
     1861 aagatacttt acgagagcga atggaatctc aatgtgtcat ctgcggatca aagttcgcaa
     1921 gtaagcagtg ttacttttgt caacataaga tctgtagctc ttgtattgtt ccagcagatg
     1981 ttaccggaaa ttacagtacc acgaaatgcg ttacatgcga tcagagaaaa ataaacaaga
     2041 ttagcattct gtcagttcta aaacgaaata agtttatttt gggtatagtg ggcggtttct
     2101 ggatttatac agtttttccg cttccttttt tacaagtttt cggaatcaaa attgacccaa
     2161 catcatttca gcctattttt ataacgaccg ctgtcatgac tattccattc cttttcatgt
     2221 tttttgcttg gcagaaacga tcgcctacaa gcggtgaatc tacaccgggt gaataaaaga
     2281 gctgaacctc atcacggtct cagataactt aatacggttt agtatagaaa tacgcatgta
     2341 atatcactag cccgcataat atcaggataa attgaggaat ctgagccgaa atcatgatcg
     2401 ccgctgccac ttcgacctgc tgacgagtgc tattgatctt gtcgcaggat tgtttttttt
     2461 agcttactat acattccctt taaacgtttt tttcatcaag tctgcagtct ttggatttga
     2521 ctttagataa gagattatct cctttgggcc gactcttcta aggaaatatc ccattccacc
     2581 tggcctgtct tcttcttctg atctgtttct tacatagtat tcgcatagca cgtcagttat
     2641 tgtagcaaca tctttacggg ggacaaggtg aaggtattgc tctcctgtat atggatccac
     2701 tagtatacca ccttgatgtc ttccatcttg tgtccccaga agtaccacct gatatagatt
     2761 aaaacctgtt cctatccacc caattgtctt tgtcgcaggc ctgtaacatt gcttttcaca
     2821 acctgtaacg cctattgatt cagtcatata tccccacttc ttttctagtt cgtcaattag
     2881 atatggttca aacttttcag agtctgtata agttagtctg catgtatctc ttccgacaca
     2941 agctccagat aacattctta gctttgagta tggttgagat accgtagtct taccattagt
     3001 aatactatcc ttgttactat cgctgtttat tttacgaaat ccatacccaa acatcttcat
     3061 atctctttca aacagttcct ttaacccctc ttctatgtta caaaacatta agtgttgatt
     3121 aggtgtagtg aataattcta tatgtggata gttattcatc aaatatttta ccatactttt
     3181 gagatccccg ttatgacttc catctattat tctaccgttc tctatgaaag cgccatagca
     3241 ccatcgactg ctattatctg gctgctttat ccatccaaga tgtaggtcta tgttgccgta
     3301 atcatagtct t
//
LOCUS       DAOR01000018           15101 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_39365, whole genome shotgun sequence.
ACCESSION   DAOR01000018 DAOR01000000
VERSION     DAOR01000018.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15101)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_39365"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    167..306
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12269..12447
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4720 a         2728 c         3104 g         4230 t
ORIGIN      
        1 gcgcagatct tcaacgacgt tgcttccctc atcctcggtg ttttatgaat tttcaagaat
       61 attgatactt gcccatgtac cgaaccaaga aagtaggtga gaaagtagat gactgtagat
      121 aagcataaaa tcactagtaa atcgtgtttt gaaacttgat agtgatnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnntgtt ggtatgttta gaccaactaa atagctgttc gccatggcaa tacgatctgt
      361 tatttgacgt gtgtgacatg tagcacgtgt tttgaaactt gatagtgatt tgccaacttg
      421 tagattggga gacataaagt tgtatccaat ctaaaaattt gatttcattt ttcgatactc
      481 tttccaactg ccagcaaggt ggtcgccact tcgctaaaga tactaacaac ctgtcctgtc
      541 agttctttcg tcatcctctt agtattaata taagagtcgg tctccttcgt tttctgattc
      601 ttcactttct cgatatcaaa atatgtaacg actccttctt ctccctgcat caatttattg
      661 tgatcaatcg atactggcga aatttgacat aattccataa gcaaatcgtt tagttctgaa
      721 aacagccgct ctttcttatg acgcatttct tcgaattccg gaaattcatt ttctagtgct
      781 attgtttttt tttccaaatc tgcgactagg cattcatcct caaacagttt ttcaagcaac
      841 tcttcaaatt ccatttgcgc atatccaata ctttccaact ccttctgcac tatatggttt
      901 cctttcccag ccatagattt gaaagtgttc ccaccctcac cagcgatctg agctaactcc
      961 ttttggatct caataatacg cttatcgact cggtaataag gcaatgcatg aaattgaatt
     1021 ccaaaatagc cagacattct gtattggccg acttcctgcg tgaactgaca atacaacctg
     1081 tgtaggtcct cggtctgcga ggtagatatg cctgttgtaa cccaccattt tagtgagtca
     1141 attaattgtt gataaaatac tgtaaccctt tgtggctcaa agcaatcaat ttgagttacg
     1201 ctgccgtctc ccaacatcgc gttaacttta ttcatttcga tttgaggctt tattgctaaa
     1261 agatagttgt cttttacgat ctcaaaggca gaattaactt ctctggcaaa ataatcatcc
     1321 tccttgatcg gtaatctcat gattagtttt gtaattacgc gttagataaa gattggctag
     1381 gcaaaattgc tttagataaa aaaatcaaat cttgtgtata actataaaag cagatcatgg
     1441 caacttgcat tctgtggaga aaaatactat tgaaaaatcc cccaaggtag aaaaaacaac
     1501 acagatcaag ggcacttaat cgtataaatt taatgtgaaa tatgataatg gggattagtg
     1561 gcaaatccga agcccactac taccgtaaca agtaacggct gatgcaggaa cagtcgttgc
     1621 tcgaagtcaa gacagtgtga tgcttcaaaa gataaaacgc ctgtttacga atggaattgc
     1681 aatgttttta ttgaacatta ctgccgagta ggattgctta tgaaaatcgc tgtggttggt
     1741 ataggtgtag ccggtgctta tctaatgaac agactgagcg atgctcacga tatctatgta
     1801 accggttttg agagaatgcc agaaacagat catgacgctg tttgcgcatg ggcgacatcc
     1861 aaaaacgtaa tgtcaggttt agcaaaaaat tgcggtctga attttgagga ttatattatg
     1921 cacgacggaa aacatatgat ggttgacatt ggagatagca ggaataatag cattgatatc
     1981 cgtttaaaag gaatggttac ctatgacaag ctaaggctaa ttaaagatat gatacgagga
     2041 acaaaagtcg aatttggcag agctccacgg aaggagaatc ttgaacagga ttttgatatg
     2101 atagtcgatt ctacggggtt ccatcgaaac tatcttccaa agttaagaaa tgaaatgtgg
     2161 atcccttgtg ttcaatacaa ggtaaagtac aaggtcgata aaactcctta cgacgatttt
     2221 tacctgaagg cattcccttc aatgactggt tatttctggt attttcccct tggcaatggc
     2281 tatgcccata taggagccgg agactttgaa agaaaacata ataacgagtt tgttgaacaa
     2341 tttttaaaaa aatacgaatg tgaaataata aaaaaggtcg gccggccggt aagaattagt
     2401 ccgccattat actgtgagcc gtttacagat ggtaggaagt cagttggagt aggtgaatcc
     2461 ataggcacgg tctattcact acttggtgaa ggaatcgttc ctgcaacttg gtgtgcagaa
     2521 ttgttgatta agaatctgtt tgatctgaaa atatatcgag aacgtgttat aaagaaattc
     2581 gaaatctatg tattggtcta taaatttatc caactcaaaa taacaaggaa attcagtttt
     2641 gtgaagcact ggagtgacct attaaggata tataagcata tgaaaaatga agaggatcgt
     2701 tatgggatgg aaattagaat cgcagatatg atgaaaattt ctaaaatttg agccattgag
     2761 gtcattcagc gtgtccctgc ctttgagttt ttccctatct ctttcgctgg tggttgtttt
     2821 tcttagctgc atccccgaac gaggacagaa tcgccatttg atagaagcaa atctcacaca
     2881 ttatacaatg tttcttacca gcaaagtagt ggactccata aacatgtatt ttagtctagt
     2941 ctttcacaaa tattttcaga gaccatcagt atagtctata aaaaaatcct cttgttaaaa
     3001 aggttaatgc agctatgaga aaggaggagg ggacgctggt aaagtttatc atccatactt
     3061 acatggttta aaatgagaag gaattggcaa agagaataac agtaacacct aaacttgtaa
     3121 aggacctcat cgtccctaga atagcaactt ctcgcaaggg tgataacggt atagttttag
     3181 tcgtaggcgg aagcaggatc tatcatggag ccccgatact tgcatcgata gctgctctaa
     3241 gatctggtac tgatcttgta tttacggccg taccgaagtc caatattata gccgtgagag
     3301 cttactctcc agacattata gcactgcctc tgcctgatga taagctgaca tcaggattag
     3361 taaatagact tatggcaatg ctccctaaga agcctcacgc cgcggctatt ggcatgggga
     3421 tgagtatcgc caagcccgaa gcactcgttt ccctgataag acaacttaga aatgcagaga
     3481 ttaaattgct gctggatgcc tcggctctaa ttcctgagat actccgagaa attgcgggaa
     3541 ctggcacgat cgtcacccct cacgcaggcg agtataagcg gctttttgaa aagcatcctg
     3601 gtacaaatga agatgtacaa acttcaactg ttcagacggc tgcaaaagag tatggaatta
     3661 caatagtact caaaggatcg gtaaatataa tttctgatgg tgtcagcgaa catactgctg
     3721 taattaaaag aagtactcca gcaatgaccg ttggaggggt gggtgatatt ctttcaggtt
     3781 tgacagtggg tttgcttacc aagtatagct cgttcaatgc ttctattctt ggcgtttttc
     3841 tgaatggagt agcagcgagt ctggcgtatg agcgagtagg tttgcacatg gtggcaaccg
     3901 atataataga agatttaccg aatgccataa aaccgtttga cctaatcaag gatcaggatg
     3961 aatagttatt catgttcagc agtcattatc aagcaggtat cccctttgat aaagagttag
     4021 tccatccaga gatcttccgg gcgggaggta agagctcggc atgatttgaa caatctactt
     4081 cttttgcatg ctaattatga tagctagcta gtcatgttca taaggattag ctagtttgaa
     4141 ttacagcgga cgctcttgtt cggttcggat tcagaacaat aagatcccaa gattcaccgg
     4201 aaattgaacc attagttaac atatgtacgt gtaatttcaa aatattttta atatttcaat
     4261 ctagtattaa aatgtgaacc cacccaacta tagtacatcg gtatcccatt caaaaatctt
     4321 attagttcaa cctcttctaa gattacctat ggattacaag cgcatccatg aaagtataat
     4381 gaagagcgat ccaaagatta gattagttac aatatgtgat caaaatggca aaattatgca
     4441 ctcggaccac cgtgaaggta taagaaattt gctaactcca gacgaaagca agaaatcact
     4501 agatcttgca gttaatgcat ggaaaacccg cagcgaactt gctccaaaga ttggaaaagg
     4561 aaaatatgta ttggcagaat atgaaaagat aaaacgcatt acaatgcctc tgggagataa
     4621 ccatcttctt tatgtaacta ctgaaagaga tgcagatcat tctgttataa ttaatagtat
     4681 cacaaagttg gacgtgcatt aatagaagaa gcatctctcc ttctctatcc gttgctattg
     4741 agattttgaa tgacagtttg tatacaggtt tacctataga ccaggactgt actttctcat
     4801 acagatgtgc taaatttcgg catataatgt tcaaggtatg cgtatttaaa agccgtaata
     4861 ttcaaccttg gataatgttt gttggcgaat cgaatgaatg ctaaaataaa actggtaata
     4921 tctggagtca tagttgccgg ggtaatcgcc agttcgttat tctatagtaa tcccgtcttg
     4981 actatcagca ataacaataa taccaacaac agtaatggca catcgtctaa tatcttgaga
     5041 atcggctatt ttccaatttt aattcatgct caagctgtta taggattggg aaatggagat
     5101 tttcaaaagg cgttgggcgc taatatcgtc gttaagccac aaaattttga ttcaggtcca
     5161 tcgttaattg aggctctatt tgcaaatcag attgatgtgg catacgtagg gccgaatcca
     5221 acaatcaatg gctatatagt atccgacgga aaagcactaa agataatttc tggagcatca
     5281 agcggaggca ctgtttttgt tgtcaggaat gattcgggaa tccagtcaat caaggatttc
     5341 gcgaacagaa aatttgcgtc gccacagttg ggcaatacac aggacgtggc tcttagaaga
     5401 tatttgcttg aaaatggcta caagacaaaa gagaatggag gcaacgtaca ggtaatatct
     5461 gcaaaaagtt cagatatatt cacattaatg ttaaagaaac aaatcgatgg agcgtgggtt
     5521 cctgaaccat ggggtgcaaa actggtaaaa gaagccaatg caagaatctt tctcgatgaa
     5581 cgaatcttgt ggccaggagg aaaatttgta acagctctca taatatctag aaccgattat
     5641 ctacgtgata atcaggatat cataatgaaa ttgttgggtg tacatgtcga tgaaacgaac
     5701 tggatcattc atcataaggc ggaagcgatg caagcattca acactcaact caaaaaactc
     5761 acaggacata ctatagcgaa tgaagagtta cagcaagcgt catcaaggat agactttaca
     5821 tacgacccat taaagcaatc tctttttaaa tcatcaaacg acgcatttga gattggtttt
     5881 ttgaaaaagc caccaaatct tgttggaata tatgatttaa cagtgttgaa taaagtgctg
     5941 aatcaaaagg gtctagcgag aatttcagat ggataaattg ggcaggaaat taggtattta
     6001 tccatcagtc gcaattatat gacggaagac tcattatctt aaaaaatact gacggtttgt
     6061 tagcagacaa atttgtaatc gtcattattg catttatagg gctaatttct agtcacaatt
     6121 ataagtagct gtggaaattg aacttggatg agcaacgaaa ttagtcgtca tcaggctgga
     6181 atggagcaga aaataaatga aatggtggaa cagtctagaa ttcttgaagg ctatatgaat
     6241 gatattatca atcgcgaagc gacagttaca aggttagtag gcgaggctcg tcttgcttcg
     6301 actgcaatcc aaaatatcgt ggatgaaaac gtagttgaag cattaacgcc agttggaata
     6361 ggcgtttata taaaaacact ggtccctcct attaaggagt tattgataaa tgtgggagcg
     6421 ggagtgactg ttgaaaaaac tagggaagat gccatcaact atgtggaagt caaaatcaaa
     6481 gaatttgaac ttgcgttgag gcagcttcta agccagaaac agcaaattgc tgtgcgcatg
     6541 gagcaaattc aggcccagat taaccaaatg ttacagcaag ggcaaagcac cgctgcatca
     6601 tctctatcaa accctagaaa acaagctcat gataatgaca acagagacag cagccgccag
     6661 cattctcact cagtttaggt tggccagctg agcatatgtt cgatcagctc agaaaggcat
     6721 tttcaagcgc cactaagagc ataggccaaa gagagctctc cgaaaaagat attgataata
     6781 tcttatttga tctgcaactt agcttgttgg aaagtgatgt tgcacaagag gtaaccgacg
     6841 atctctctgc aaaattgaaa aaagaattac ttggattaaa actggaaaaa gatcagatta
     6901 aagaggaatt gatcagatta aaattccaaa ctgttataac agaaatgttt gcgaaaaccg
     6961 gaaaagtaga tctaattgac aaaattaaag taaagaggga attaaaaggt ggaccgttcg
     7021 ttgtcgtatt tcttggaatc aatggaactg gcaagactac gacagtcgca aaaatagcca
     7081 atctgctacg caaaaatggt atttctgtag tgttggcagc tgccgatacc catcgtgccg
     7141 gggctatcga gcaactaacc cagcacgccg agaaattagg gttaaaagtc attgcccaac
     7201 gatacggagc tgatccttcc gccgtgggta gggatgcagt tgattatagt aagaagcatc
     7261 atatcgacgc tgtgctagtc gacacagccg gcagaatgca gaccgccaaa aatctgatgg
     7321 acgagatagg taagatcgtc aaagtagtaa agccagatat aaaattattt gtcggtgatt
     7381 cattagcagg aaatgatacg ataaatcaag ctagggagtt ttttgaatat actaaatttg
     7441 acggcgcgat attaaccaaa acggatgcag atgccaaggg tggttctgca atttcaatag
     7501 tttatactac ttccaaacca attgtctacc ttggagttgg gcaggggtac gatgatataa
     7561 taccgtttga catgggtaaa ttccttaaat ctctattcgg ggacgcgcag aaaattaata
     7621 tagacaattc ggttttgaga ctagaacaaa cctctgaacc tattggggct gcaaaatcaa
     7681 aggtggaact tttgtctagc agcctatctg ccaagccagc tgacgaaata cgaaaacctg
     7741 tgattaggac aaccggtgct aaggagacta gcgatgccgt tgactattcc acatcgccag
     7801 cagaggaaga aaaatcaaaa tcctcgccat caactctccc ggaatcagta atacctgtgg
     7861 cagagccgga gaacaacaca aaacctgcgt tagaggttga atctgaatct acacttttaa
     7921 aagacaagac acaggtgtct gaaacaccat attttttaaa ggaaagccga gaagacattg
     7981 atctggcaca tactcacgaa gagctagaga aaaagaagag tcgttttagc aggcttttca
     8041 gtagaaggaa gaacgacaaa aaactaggaa tagtcgaaga acccgatacc aaaatgacag
     8101 ctgagaagga caagatgata caagatacgg acgtggaaag taaagatcag agaagggaaa
     8161 agaaggattc aaagcccggc gaaaaagaag agcaaggaag ggacgccgac gagaaagtag
     8221 tatatcttag cgatgaagac attgaagacc tgcttaagta aaaaagacgt tcttagtctg
     8281 atagacgtaa acccagaaga aattttttca atattaaaat tagcaagccg ctttaaaaaa
     8341 gagtcaaggc gtagaaaatc agactcatta ctcaaaggaa aagttctggg tatgattttc
     8401 caaaaaccat ccaccaggac tcgcgtaagt tttgaaacag gaatgtatca actcggtgga
     8461 aatgtagtat atctaagcac taacgatatc caactggcac gtggagaatc tatagaagat
     8521 acggcacgat ccttgtcact atttctaaat tgtatagtag cgcgacttta ttgtcatgcg
     8581 gacatacaaa gattagctat gtacgcttct gtccctgtta taaatggact ttctgatact
     8641 ttccatccat gtcagatact tgcggacatg ctgacgattc aggagcagaa gaaaaaacta
     8701 gaaggattaa atgttacatg gataggtgat ggcaataacg tttgcaatga tttggtgttg
     8761 ggttgtgcaa agactggcat gaatatagcg gtagcttgtc ctcgtggcta tgaaccttca
     8821 cgccaggtgg taatattggg aaaaagagaa ggacaaaaga caggatcgca aataagtatt
     8881 acagacgatc ctgcagctgc cgctagaaat gccgatgtcg tggtgactga tacatttatc
     8941 tctataggta gaaatggaga aaaaatcact cgtaataagg cctttcttcc aagataccag
     9001 gtaaacactg aactgatgaa gttggcgaaa aaggatgcca ttttcatgca ttgcctgcct
     9061 gccacacgag gtcaagaagt aacctcagac gtaattgatg ggaaggcttc agttgtgtgg
     9121 gaagaggccg aaaacagatt gcacgttcaa aaagcactaa tgtgcttgct gataagtact
     9181 aaaaaaggag cttaatcaca acctattctc agtttttggt ccgagcgcta gaaataatcg
     9241 actaaacgaa gttgctatag tatagaacaa tacggttttt tgagctgaag tgcttcgcga
     9301 caattcctgg aagggtcatt ctataataaa taattatctt tgagaaccag ctagtcaagt
     9361 cattcacgct gagaatgggg ttaagctaag attcttcaac acttagtaga cgataatttc
     9421 tggaccatcg gacaaactat tattataaag gaatttattt cgacttttcc aaatacgatg
     9481 agattttaaa tatggcaatt tcggtgtagc tccatggcaa aggttgttgt aggaaaaact
     9541 tctgatattc cagaaggaaa gttaacacat gtaacagcag gtggaaagga agttctagtt
     9601 gcgaatgtag ggggaaatta ttatgcaatg ggtgatactt gcaaccatgc aggagctgag
     9661 cttcatgagg gggagcttaa tggcaaggaa ctgacgtgcc cttggcatgg tgcaaaatgg
     9721 gatgtaacga acggcaacct attgtggttt cctgccaaat taaaccctga agagtcattc
     9781 gatgttagta tagaaaatgg gaccgtctat ctagaggtct aataataaat atacccttga
     9841 agtggctact gagccggcgt cttgcattac ccacaatttt ttaattcaca gtttcatttt
     9901 tttttcttta aggtatttga tttatcagct ctttcatcta atctatccta atcgtggata
     9961 ttattcgctt cagtcccttt gttcgagact tgaggtgaca tttacaaaat taacaaaagc
    10021 aagaaaattg acatgatcag gattggaggc tatgatggca gaaagtgcat caggatatga
    10081 atagtggggg aatgcctcga acttcatcta aatgcgctat tgtttatgct catcgcttgc
    10141 atcatccgtc actgcaggca tggtgtttat gattcaaaaa actcgagacg aaacaatacg
    10201 tacggtggtt cgccaaatgc atctattgcc aaattaggct tgaagaatgg tccaagcatg
    10261 atagaagatg aagttagagt tgatgacttt tgtagacaaa cggtcttaca cagattagca
    10321 actatgattt atcatatcag aaattggata aagtacaatc taactcgggt cgaatttaaa
    10381 tttaagacag ctaaggcata ttttgtacgc gatgacaaag tgaagcccag aatgtgatac
    10441 taaataacat attttgcaaa tcgtctggat cgggggacat tcggggattg caaacgtttg
    10501 cttaaaggat aaaaaatttg atatggaata gagcctcagg agcatctagg tattgctgcg
    10561 ggatatgttg catctatgat aaggccattt cacatctagt cgtttaaaga ggaaggatta
    10621 tcacagagtc tataatatat aactcacctg gatttcgagg attcgagatt gaacctgcaa
    10681 gaacttattt tatcaagagg ataaaggcta aaaaggaaat ctaaaaagtg atattgtgag
    10741 accatacaat ttggcagtct tactacattc ctattactat tctgatggta tcttcttcgg
    10801 ccttgacatg atataaaatc cgtgagccga aatgagaaat gctgctaatg acatctttgt
    10861 cgcaaataca aggttccaag gtgttttggg attaggatat ggcatattta tggcatcgag
    10921 cttcaatatt cccttcatag catcagctgt aatcaaagaa ctccatatta tgaaattgta
    10981 aatgaattca tatagtgcta ttacagcaat agctagaact actagttgta ggatcaactt
    11041 gactgtctta ggaacatctg tgaccttctc tccacccaac ttagtcacac aataccatcc
    11101 gacgacggac acgatcatta aatatgtcac aagttttgaa aatccatgta cagggaattc
    11161 tgtgttaacg agtacttcac caattacaac tcttccagtc ttaagatatt ctccgacact
    11221 gacgtacaaa gcgtatgctg taatagacgc cattgcgaat gcacaaaagt aaaatattcc
    11281 aagaaggagt ttccgtgacc ttctttcatc tatttcaagg cttgagtggc gcatagacct
    11341 acagataatt actatgtact ggagggctat aaaaaattac atctccgggc ttgggaacta
    11401 taattcttct aaccacctgc actgatagta cttccaatgc ctttacagac actctttatg
    11461 agcttattgc ccaatttctg acttttctgc ccaaccaatc ctccattggg tgatgataaa
    11521 catcgaaaac acgatatgaa ccgagtcgat ataaaatata tatcttcttg ggcctcgggg
    11581 ccttctgtgt tgatccttta tcgcctctat tacttcaggc caggcctcct cctcccgcag
    11641 tgatttatat ctctaacaag attcgtgttt gcacaatagt ctaatatact aactggcatc
    11701 gagtaatctg gaaaggaaat gaagagacca gattgggata cgtactttat gcttcattca
    11761 gaaatagcaa aactccgctc gaactgtgtt acaagacata tcggtgcagt aattgtaaag
    11821 gacaatagac aaatagcaac gggctacaac ggaactcctt cgggtgtaaa gaactgtttt
    11881 gagggcggat gttacagatg catggcgaga atcaggggcg aaatcaaatc aggtgaatta
    11941 ttggagcgat gtatgtgtac tcacgcagag gcaaatgcta taatgcagtg cgcattattt
    12001 ggaaatgctg gaagcactag gggcgcctca ttatattcaa cctttgctcc gtgtctagaa
    12061 tgcagcaaga tggcagtcag cgtaggtatt aagaggatag ttgttcttgc tgactaccca
    12121 gaagatggga cgcagctgct atctgaggct aatatcaaat tagtaaagct aaacccggac
    12181 cttctacaac cttggatagc ggttataacc aaggatccag aaacgtcggc aaaagaggca
    12241 aatacgctgg attcgtatta gaagatgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa ttagttgtca taccgccttt acgatttttc
    12481 ataatgcggc cacaatttct ttatagcatg aatacgataa taacaataac taattaaaac
    12541 tactactctg tcgtgatagt tagaacagct aaaaaatcag ataaagaaga agttctaaag
    12601 ttttgtagca atactttcga atggggcgat tatattcatc aagtatggga catatggtcc
    12661 gccgagcagc atagcaagct ttacgtagtt gattacgaaa gaaggaagaa caataataac
    12721 ttcccggtag ctatttccca tgtcaccatc tgtcctggca aaaggcagat ttggcttgaa
    12781 ggcatcagag ttcatcctgc tcacagacga aagaagatcg ctaccgccct aattgcaaaa
    12841 atgctgcagt ttggaatgcg acaaggagcc attgaagcat cagctgttgt agcaactgac
    12901 aacattggat cacggctcat gctggaaaac aatggtttca gtgtaatctc acgatgggtg
    12961 tattacgcaa gtacccataa agctgtggga tccagtcgaa taaggcctac agacgcaagg
    13021 gttgcctctt tagaagattt aaacgacgta tgcaattatc taacagattc tccaatatac
    13081 aaattgtcag gaaaaaggta cgttaaagcg tggcgttggt attttctcaa tcgtaatacg
    13141 ctgcggcatt ttattaaaaa gcgaaacttg atcgtgacag gctatccgtc tcctaatgga
    13201 atcgctctac ttaataggtg cggttattgg cataggagag atgccttgca aatagtgtat
    13261 ttagattcct tatcaaaatc ttccttacaa aatctcgtcg ctttcctaac aaacctacgt
    13321 atctcatcaa tcaaaccgcc gcctaattct cattcatatg tcttacaatt tctagcttat
    13381 cagagtgagc acttgccagt cgttatgaaa aaatttgata ttaaggagtc ggagcaattt
    13441 cttttatatg ccaaaaggct ctgattgcat agaatagaac ttaaagttgt gtaaggtgag
    13501 aatttggcga taaattcgct tgtagattat atcaagaata agattttatt agactttcaa
    13561 attcgctgta aataatgagt agtagcagtg ggagtaatga caaattcaac tttatgcttt
    13621 ctgagattga gtggcaagta gtgttggatg ctctcagtaa tacaatattt agcaaggagc
    13681 tcacagacga ggctaggaat aatgcgaagg aattattttt aaaattacaa gaacttccaa
    13741 gaaggtaatt aaaaaccata aagcgctatt tcaagctcag aatccggagt actttgacct
    13801 atgattgtac atatgtttaa gattattttt tattaatttg ttcacggact tgtcttatta
    13861 tatcaagaac tgcatctgcg tcttctgctt caggatccag ttcgaggtac atgttcaaca
    13921 tctcgagtgc attcaagggg ttgttcctct ttagaaatat catgccctta tctcttactg
    13981 catcggggtt atggggctct atggcaatta tcatctcgtt cgctatttct gctctctcga
    14041 tatcttgcga ttcataataa ctgcctttta aattatttag catccgtatc atgacctgtg
    14101 ctgatgtagc cttatctaca aacgctcgtg ttaatgcaat attttgaccg ggataaaatt
    14161 gatcgagcaa ttctttcata gcgtaatcat ccattattcg tccaccatta aacgggtcga
    14221 taattatctc actaccgtct ccttctagta tgtgtttaat cagaaagtga gcaggaaaat
    14281 tgacagcgtg taatctaaat tttagaatat gtgctatgcg tatgtatatt atagacagag
    14341 taatggggat accagacttt tgttccaaaa caacgttaac atagctgttc aaggggttat
    14401 agtagtcttg taagtttgct ctaaactcct tctgtttaaa taaatagttg ttaattttct
    14461 ctattatttg cgtcggcctt aacggcatca atttcttcgt tgctaagatt agctctcttc
    14521 ccattgcgtc gattttatct aaaatatctt ttacatccag gtcgggataa gcaagaatcc
    14581 ttgaaacgtg caaagcatac tcagctagcc tctccgtatc gttaccatca ccaaggaaat
    14641 taacaacatt tgctttccag tcttcaatta aaggctggag attgttaata gacaacaatt
    14701 catatgagaa attttaccta cttataattt tggtgtatta tatgtgacga gttccgtagg
    14761 cggtaggaaa gtgggtagag ctttaccagg tagcagttta gctgctttct tcagtatcct
    14821 ctactgagtt aatcggggac aatgacgacg gcggggatga taagaagtca tctcctatta
    14881 cctctgctct tagctgtgta ctgttaccac aattattatt attcccacct ctagtttgtc
    14941 tatatcttcg ataatgctga tccacttcca tagctgctgt aagtattttg cttcttatag
    15001 acttggatga gtgcagaaac atctttgtat cttttggcag gcagtgacct cctattcctt
    15061 ctcttggttc taaaatattg acattccatt tagtatttag a
//
LOCUS       DAOR01000019            4872 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_72590, whole genome shotgun sequence.
ACCESSION   DAOR01000019 DAOR01000000
VERSION     DAOR01000019.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_72590"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1558 a          985 c          906 g         1423 t
ORIGIN      
        1 tatatcgaca atttctccta tattgttaga agttgctgtg aagcatctca aatatactat
       61 attgaatgat atccctccga gaaatgaacc acagcaagaa gactttaaca actgcagcaa
      121 tcgcgattgt agtagcacta acgggagttg cattagctat accacaagca gctttggcac
      181 acatacccca gcatctgttt gctactggtt ctcacgggat taatgttgat ggtgctaatg
      241 gcgctaacgg ggttaatggt ggtaacggtg ttagctcccc cggtgctaat ggcaataatg
      301 ctaacggcgc gaatggaaat aatgataacg gcatgagtgc taacggggtt aatggtggta
      361 acgctaacgg ggctaatggc attagcgccc ccggcgtcaa tggaactagt tctaacggtg
      421 tgaatggtgg taacgctaac ggtgttaatg gtactagtac tagcactact gtgcccaaat
      481 ctgggttttt caattgaaaa cgccaccaac ttgaatgaag aggaataata tgaaaacgat
      541 cttcgacatt agtagaatcc agcgcagata aacacaaaat cttcgttctt agcaaagatc
      601 acagactaaa gtgtcgatgg ctgtccaagc tcattctgat attaaccgcg tgataaccaa
      661 ttttagaatc ggaccagcac gtgtgatcag atagaaaata aaaataaaaa taagtaaaag
      721 agactgccac tgcataagtt aaactacaaa gctgtgaaat aactacgact aaatcgtacg
      781 ctcgtggata ttaatgcact atgctagtgt tgcatacttt gacagtctat gatatttctc
      841 gaatatactt ttctcttccc taagggaaca gaattattga agtttattct gaatgcgaat
      901 ccattagctt tttacaggtt ctaattgttt aaaactaaaa gagtgtagac cggcttatat
      961 gaaagagatt ctggatagga gaactatgaa ccacataata ataaactatc cctaatcctc
     1021 tctgtttttt aagccaagtc tacacctaaa ttattattac gcaatagtag aataaatagg
     1081 attactaact ctacatgcta gttataatcg acaagagata tctgttgcta aagtctcttt
     1141 atgaattcaa gcgacattcc gactataatg tttctaatcg taactttagg aatcctagct
     1201 atcgttcgtt actaccgcca cggcactgag ggtagtggcg ttcgttttac cacaacaggc
     1261 tctagcatac agacaccact accacaataa tataatacca caacagcaaa agcatggcag
     1321 tcaatcaaca cacgagtcag ctaaacaact gtaaagtaca ggtacgtctg caggcaatac
     1381 tggcacagat gattcaagta cagttcatgc acatgaggaa gaaggtcagc catcaccaac
     1441 tgtctgccaa aatgcgtgca ccaatacagc tgaaataagc cgcttataac atagaagaga
     1501 acaattcaaa aatatgttct tctttatttt tttcctgaac ataacgacaa aaaatctttg
     1561 taagtacact cgtcgtaact aatatcgcag ttttgtgacc attattacgt cagctagcag
     1621 acctattatg ttgattggag tattactaga cttacaggtt atacccttca tggtattaca
     1681 gtgctaccat taacacgaga aattctatca aatatcacaa taataaaaaa tcagttacaa
     1741 aacttatcgg aatcaagtgt tagagtttag cctgcatagt acgcaaagtt gagacaacac
     1801 acgtgtaacc tttaaaaccg tagattaagc tcaaacttta atcgaagaag caataacaca
     1861 tgtaaatcta gttagcgtat aaagtactag atatatttaa cgcaactaaa ttctaaacat
     1921 tttataatga gaatcatatc tagtcttttt ataagtatcg ctctcatgtg aaatatatgg
     1981 tgactttcaa tcttagatcc gacaagttag tattctccgg tatcttgatc atgacccttt
     2041 ttatatcaac tatctggttt tcatctatcc aagtcaacag gatccttgct ttgccagacc
     2101 aacaacaact aaactccaca tccaaacctc tcgtagtaaa aattacagat cctattaaag
     2161 gccaacaaat agcggtcggt aaaaacctga ctctttctgg catagccaag tacaatgtaa
     2221 ctgccaactg tcaggtttcc gtaatcgtag acagtatgag accatatcaa aagacaatac
     2281 caataggaca gactggagtg ggtaattatt ctcaatggaa gtacgcactt gctccaacat
     2341 atgccggtag cattaaagaa ggaagtaata aaataaccgc aaagttactc tgcaatacaa
     2401 atcctacagc ccttacaaaa ttttacagca ttaatgtgac aggtgtgaat ggaatttctc
     2461 cgaaccaaca ccaacttgca ataagatcaa acaatactgc tgtaccattt ttcctgccag
     2521 tgtcttcttc tttttcttcg gctccattaa acaataccct tgcacctgct gtcataactg
     2581 tttcagtcaa caacggtaca ctacttccgc caacaactgc ttccaccagt agttcttctg
     2641 gttctagtga caccagcagc ggtagtagca atggacatca tcatcatagt actagcagcg
     2701 ccaatcattc gactagcagc gccaatcatt cgactagcag cgccaatcat tcgactagca
     2761 gcgatcatca tagtagctct agcaacaata gtggcaataa cggtaacaac catcatcatg
     2821 acaacagagg aggaggacaa ggcaatttta tccacagcat aattacacat ttcaggggat
     2881 tttaaggcca ggtcagtagc ataaattggg aatcgtgctt tttttcagtc aacgaaaata
     2941 atgaatttgg aaattgacta tgcatagaac ataaagaatc gtttatatat aaattgccgt
     3001 cattttgtta ttgttgtggt ctccttgcag acgaatatgt gatatatgtc gggttcttgg
     3061 atcttttagc gtaaagaatt ctttgttgaa tgcgtcatga aaatttggcg cgccatcagt
     3121 aatgaatgta ttcggtcttt taccagctag ctcttttgca ttttcaagta gtggtctaac
     3181 gtttgatgta tatttcgtgt cagctacctg ctgtgctatc caaaatcttg tttggtcatc
     3241 catgagtgca tacaaatatt ttggatttcc tcggactttc aaatataatt cgtcggctct
     3301 ccaagcatca gaaacatttg gcgtaatttt ttctaagtat gattgcatta aaccgacgta
     3361 ttttttcatc caccgataaa cggtattatg attaacgtta acaccttgta atttcaagaa
     3421 tttttgaaca ttgcgtaaag attcgcccgt aaaatatagc tgcaaagcag acgttataat
     3481 ttggggagtg gcgagcattc tttcaaatcc caaattgatt gtgaagtagc gattgcaatc
     3541 tctgcattcg tatttttgaa tatcgccgcg tttgttatgt ctgagtccgt cttttacgat
     3601 attagaagaa ttacaaaaga tacagcaatt aatttgaagt ggttctattc gcgcaatttc
     3661 gacttcctta cgtaatgcaa atgatatttc tacgacaaaa acgtgtttgc atttcacgcc
     3721 acgaaatctg ttgtcaggac atgagcaaac ccagcctaaa tccgtcgagc aaacatcata
     3781 gtctccgttg tcattctgcg attttacttt gtatacggat tcgctaattc tttttattgc
     3841 gccgctaatg ttagcaatga ctaatccttc tggtgacggt ttatagctgt tttgctcata
     3901 tatacataaa gacaaatccc cggcgacacc aatccgacct agaaaagtga gcataaaaaa
     3961 ttagaaagga ctgagcatag tgatgtctat ctcacgctgc tgctatccga tttcttaatt
     4021 tcgatcgtga tcttattgcc cactcttatg gccatgtctt cgtactccct catactgaat
     4081 ttgattgaag gggaaaccgt tagcatggcc ttagaaatat tcgccatcgt catcttgggc
     4141 aaatttttca tcaaatccgc tggtgaagta aatgctatca cgtttggacc aataatcgtt
     4201 atatatggag cgactagagt ccctttatgc aaaaaaatag aatgtttacg atggcatgtt
     4261 taacgcttgt aatatgcata ttggcagtaa gcatcggcac aatcatcaag ctgactgcta
     4321 ctacatcttt cgcattgact gtggctgctg gaagtcacac gggtggaaaa attatgtcag
     4381 ggggtatgac tggtactaat acgacaagtg gcatgatgtg cacgccccac tatccatgtt
     4441 aaaataatac caacacgaca gccagtaaat aggagcagta gcagccgttg ccggagtatc
     4501 aagtatacct taattatttt agccacatga gacgttaaaa ggaaaaccgt ctgccgaaaa
     4561 aaggaagtat tattgaaggc ggagacaaag ggaaatcagt aatcgtgaga ttgcctgaag
     4621 aaaccaagcg aattcggatc gcgagaataa accgcttgct cgaaacatca aaacaatttt
     4681 cttgatcgtc atctcgatta tcgctaaatt taattcttaa tcgcgtgctc tatattttca
     4741 agcgaataat gtctctatat gtggtctcaa gagtatattt gaattaaatt cgcgccaggt
     4801 actaattgaa attttttgaa ttgctactta acccatcaac aatgcttgag gaatttccta
     4861 gagaaaataa ta
//
LOCUS       DAOR01000020           11023 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29096, whole genome shotgun sequence.
ACCESSION   DAOR01000020 DAOR01000000
VERSION     DAOR01000020.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11023)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29096"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3358 a         2180 c         1995 g         3490 t
ORIGIN      
        1 gcagaaaagt gtcacctatg tgggtatttg aaagtgtcac ccatctatgt aagtactaca
       61 actaggtatg aaaagaactg tatactactt aataatacta gcagctgccg gtttgatatc
      121 tctctactat tactctacac aaataaataa gcccaaatat gctctcgaac tcgatccaac
      181 taaggacact actgatatgg caggtatcca ataccgtgtt agggttaata atgttggatt
      241 gagtcagctt acagggatca cagttgtcct agcaaagaat gatactcaac atctaagctt
      301 tctagatcca ggtcaaacat actttttttc tcccaaacca gatactaata taactattgt
      361 aaaaatatct accaatgagg gaatagtggt tcagggcgat tatcgttcac caacaaaagt
      421 ccttggactt cctgggagtg gaagatgagt gagatggatt gcatttttaa gatcagaaat
      481 agttatctac catcttctga tgttaaacga tctgcaaatc tacgcatttc ttcgtattag
      541 cctcatgcgc ataccatatt tggggcgcaa tgtgacaagt ggttggagca caattggatg
      601 acctaattct agtttcatct tccatctcct aacgataata gcgatcgcta gtatactctc
      661 agtcaatgca aatggctccc caatacaagc cctggaacca ccgccaaatg gaaagtagct
      721 aacctgggca aatttgttct tgtctcacta ttccagcgtt caggattaaa tcttccaggt
      781 tcagggaaaa agcgtttatc acgatgcatc aaatattgac tcattagaat tgtcgagcca
      841 gcaggtatgg tataccctcc aagcttacaa tcttgtattg cttgacggcc tatggcccac
      901 gctggaggat aaagtcgcat agactctgaa aggacttttc ctataaattc taacttcgat
      961 atgtccgcag ctgttggaag ccgatcgttg ccaagaattg aatcaatctc ttcataaagc
     1021 ttctcttctt ctctattgtt ttgagagaga aggtaaaaag tccatgttaa tgcattggct
     1081 acagtctcat gtccagccaa aaaaatcgtg ataacattgt ctcttagcaa ttttgagtta
     1141 acgtagttcc cattgtcaga ggtaatgttc ttgtttgcaa gcatgatgga tattagatcg
     1201 ccatgatgat tatgacgagg caaatccttt acgtgggtaa tgcgggtgct ttggtgctca
     1261 ttgattatac gataaattag tgtatctagc tgcttcttag catagtgaaa gcgtctattg
     1321 cttggtagag gtagcttttc aataagctca cctaacggca ttctagcacg gttgaaatat
     1381 tccacaagag tcgtgacgtg gtcaccaatc tcttcagctt ctgattcaat atcaaaacca
     1441 aataaagcct tacaaacaat agccagcgtt agctgcataa attctttatg tacatccaat
     1501 gtatcatcgt ctttccaacg attagatata cgcaaaatat attctgtcat agtggttgca
     1561 taggttgtta acctgtcttg attaaaagca ggatgtatta gctgccgctg gttacgatgg
     1621 gtggtccctt cactcgttaa caatccctct cccaacactt tctttgtcaa ttgtaatccg
     1681 cgacttttta tgaagttggt attgtaagtt actaatacgt ctctaatgta gtctggatgg
     1741 ttaaggaaat agatgtgttg gttcccaaat ttaaaatgag aaatgtctcc atatcttgag
     1801 atatcaacca gtactccaat aggatctctt agaaaattaa gaaattggaa caacggtaac
     1861 ttctgggtgg gaccaggtgg ataatccata gaagttgcgc gcttcatgtc tgtatatgat
     1921 acttttgcgt taccaattta tgaataataa agtatttaat cttgataaac gtatgcctga
     1981 agctttgcta taatttcgta attgctatga atatattcgc tggaccatgt gttgcagagg
     2041 tatcattata ttgagaaatc tttccataaa gtacaattct agcgaatgtt ttcatgttcc
     2101 aaatgttttc cacctacatt gtcaagtaaa ggtcaattcc atctgaggag cgaatgctct
     2161 tcttagttat gtagagatgt tatcatctaa taattgcttg tagttaaatg cgaagtctat
     2221 gcgtgcttcg tcaataacgt acacgcgaca tatcgatatt gtttattaga attatcggcc
     2281 cagagtattt tacatccttg taaaaccgat tatggagtgg atatactctg tagagccttc
     2341 ccggctatac tcattataaa cccgcttcca cttacgttga ctgagatgga tatctccagt
     2401 ttcgccggaa gtttatttag gcttaggtta cgaatggctc cttataagat ttgatcaact
     2461 ttgagatttc tggtctcatc aagtgccctg caggaatttc accacaacta accatttttc
     2521 tcatctttgt accgcttaac tcttctctga attctgatcc gtgcggacaa tttctttcat
     2581 ttgcgtagct caagcattta ttgcagtagt aaaacgaagg aaagaatagt ggttcaattt
     2641 cgagatccgg ataatcactg aagatgtcat gcgctgcaaa aggattataa tagtctccaa
     2701 ctcctgcatg atcccttcca atgataaagt gagaacatcc aaaattcttt ctcataatgg
     2761 catgatgaat tgcttcctta ggcccagcgt acctcatctc ggtatgaagg gttccaaaca
     2821 tagtcctact cttgggataa tagttttcta taagtgtctc ataagttctt acaatcacct
     2881 catctttgaa atcaccagac ttttttttgc caatcaatgg atttacaaac aatccatcat
     2941 aaatattcat ggcagtcttt tgcaacattt catgagcaac atgtggaacg tttcttgttt
     3001 gaaaaccaac cacagttttc catcctctct tctgaatttc tgatctagtt tcaaagggat
     3061 tcagtctaaa ctttcttaat ggtgactgag ggattctcat taagacttga atgttgcctc
     3121 cgataagtcg atctttcaat cctatcactt tcataacgcc tggatgcctt agatcatttg
     3181 tttggtagat agctcttgca acagaggttt tatcataaga ataaatttct tcgacatcta
     3241 taactccaaa atgttcatta ccagttctta acataacttc accagcatcc ttcatctgcc
     3301 ttgctgtttg gtcatcaaca tctaatatta tagggatagt ccaagccaac ccattcgtta
     3361 agcgaccaga ttttacgatg ccttggaagt catcttgtcc aacaaacccc tccaaagggc
     3421 taaaaatacc atccgcaata ttctcaatgt catttctaag atcgttattg acgtcaattg
     3481 aaaacactcc atcaatattc ttcttggtag aagcaaatct gcttattagt ttacctccat
     3541 gtgggcgagg tatactacta acattagtca aaatattccc tattttttgg attgtttatt
     3601 atcgtcccaa tgtagtccac attctttatc tgtaccagtt tcccaccacc atcttccaga
     3661 cctgggatcc tcattaggag ggactgctct tgtacatggc tcgcagccga tgctcatata
     3721 accaatatcg tgcaacttat tgtaaggaac gctattttta tgaatataat cccagaccat
     3781 ttcattagtc caatctgcca ctggattgac tttaacgatg ttgttatggg aagaatcaat
     3841 ttctaccttc tgaatgtggg ttcttgtagt tgcttgctct cttcttaaac ctgtaatcca
     3901 accgtcgagt ttacttaacg ccctgttaag agaatgtact tttctaattt cacagcaaag
     3961 ttttcgattt tcgacgcttt catacattag gttcatgcct tttatttgaa ccatctgttc
     4021 aacctctttc tgctccggaa aaaatacatc aatctgaata gcgtatttct tgcgaattgt
     4081 atccattaaa tcataagttt cctgatttag ccgccccgta tccaaggtga aaattctagt
     4141 tttctctttg ttaattttta tcataatatc aatgagaaca acatcttccg gaccgaaact
     4201 tgatgctagg ccgattctta atccgtaagt atccaccgcc catcgtaaga cttctcgcgc
     4261 agattgattt tccatctgca gtgcgatttg cctcagctga ggaatagtcg gctttgattc
     4321 catagcctct tttcctatca cagatgattg taagcatagt aggcttataa ttatttgtgt
     4381 tttaaattga ctaaacctaa attcctctac gtcatgagtg attttttcga agaaagagtt
     4441 gccgagtaac tctactatat tttattatct cagtctgatt tttgaaaatt tattaaatta
     4501 tactgtgaag tttcgtttat taataagtgt acttctatat aatcgtataa taaacaggat
     4561 tgtctgagtg atctatgatg agtgaaaact tttggagatt agtggaagaa tatcaacaac
     4621 ttggttttga tcccttacgc tggatcccaa cctgctcgaa tgaagtagat agttatatcc
     4681 tacaagattc attaaaagag gtcaaaagaa gtaatataaa gctaacacct agctggtttg
     4741 atagttttta ttatacagag ggaaagatcc ctgagcttac tcgaagagta tacaatttcg
     4801 aaaatcctgt gattgaaaag gaagttgaaa ttaaacgagc actttcaatc ttccgcattc
     4861 atactgttct aggttatgat ccagcattac tctcaggagc agttcaaaag ttcttcaaca
     4921 cttttcattc aaaggtgtgc ataagaaaaa acaccatggc tttgacaacc cgtcgtgaag
     4981 cccaatttgc acttctagat tatatacagt tacacagagg cgacaaagta ggatatacat
     5041 ctgctaataa ctctgtgaca caaataactg acccgacgca gagaccagaa tctgaaattc
     5101 acagcaacat tgcattgacg gccggaatgg aaaatctcaa tctcctcgga tgcacattag
     5161 gattcaaatt gtttcctgta tatgatgcgc ctaccgaaca gatgcttgac aagattagaa
     5221 taaatcttga ttcatttact tctcgttata atatagctat ggaagactac agttccatta
     5281 agattggcaa tctctttttt ggtacaactg caattgcaaa cactgtaaaa gaattaccta
     5341 caagatacga tcaagtggag gaaggcatgc aaattattct ttcaagtaaa atgggagcga
     5401 tgccagcttt aagcctatac atgttaacac aaatggatca taataatata gtcaaatttg
     5461 aggaaaacgg catttcgtta gatgttcttt ctgctgcaaa agatgaaacc ataaaaagtc
     5521 taagtgaacc gcacttttca ctaggaaaag taatatcaaa atattgtcct gattttggta
     5581 tggcatatga caagaatacg aatataacgg ctgttcatcc tgttaccaca gaaggtttct
     5641 ttgccatagg aaaacttgca gaactgacga actctcacat tgtaattaac gagcttccaa
     5701 tgaagtatga agaaatcggc aagtttgcaa caaaggaatt gctaattgaa aatgcaactg
     5761 cctcttccaa cggctgtcat cttatagttg caacaaagga cgtagcaagt ttggttgtag
     5821 atgatctgcg aaaacacaac tttgaaccaa caataatcgg attcttcgca aagaaggaaa
     5881 ggcctgccgt gacgactgaa aaagatatta cccagtatat tgcctccaaa tcaaaacttg
     5941 ccagactaaa ttccttaaca caaccagagg caaagtagta agattaaaac taccgtacgg
     6001 atctttaacc ataggagctt cttacgaagc tatcacttgg caaatattct ctagcatctc
     6061 tcatatagcc gtcagcgtct gcttataata catgaatcct atatcgatat gggaggtttc
     6121 actttaagtg cacagaatcg atctaggtcc gaggtatatg cactcagccg ttcagatttg
     6181 acgcagcaat agatggatct aacgacaata caactacaaa taatgctgta ctggatgtat
     6241 aggacagagt actcaaagcg ttctagtccg aagctgctaa aataaccgaa aacattctgt
     6301 aattaagacc ctttctcacg aaggtttgtt aacaacaaaa tagataacag agtaacctga
     6361 gcgatggcac tttactgtag tcattactta aactaaaaac ggatgactgc caatgttgta
     6421 actttctgtc catatgatat aaccagagca cggaccccat ctaacgttag taccgtacac
     6481 catctatttt accaccatta caggacaatg tgaatatgtt acaacaccca atgcaacgct
     6541 tccaagcaat aatcttttaa aaccggttct tcctcgggtg ccaataacaa tcaaatatat
     6601 accttcccgt tcagcatagt ctactatcgt agcctctact gatgtagaac cttcaacaat
     6661 ttccgttccg aggtctatgc cttctgcatg accttctaat cgaattctat ctaaccattc
     6721 ctgagccgcc cttttttcct ctttaaaccc ttctactcca taagtaggcg cggtaatgaa
     6781 agtcgaagat acagatctaa ttccgtgcat atgaagaacg aatatagcga ttaattgaga
     6841 gttgtatttt tttgctattg caatagcata acgtgatgcg tcaattgact cctctgagcc
     6901 atctattcca actaatatct tgggaaaccg tccctttgcc ttctcctctg acaattattc
     6961 ctattttgat tcatctgatt aaaaaactta cggcaaaagg aagtacgagg taacaggatg
     7021 acgttagtta atttggaaat agtaaatggg cttatttagt cattgcagac ctatggatat
     7081 agaactagga gtgggagtag atagtgacat cgctatgatt atactaaata tacagtattc
     7141 tatggactca aaagtacaaa catgagagtg cattaggcta acacgaaaca aaaataatca
     7201 tgataatatt gacgccagag ttagtaggat tatcaaaaac cctttgcaag tgttctacgt
     7261 ttcattagtg aacgttttgc tggtgtactt tccatgaagt cattttgtga atttctttga
     7321 cttcatgcag ctatgcttgt aaatgtcaat tccttgcttg gttttcattt actcataaat
     7381 tgtaattaat gcattctgtt acctttatat ctctggtttc aagggagaag tcctatcgcg
     7441 ttgctttcct cgcgaatatc gagcccaatt aattactcga ttatattgtt ggtatggttt
     7501 gaattctatt gataggggct ttctcattat gaaaagatac ttgaaaccac gtagatagaa
     7561 gttgaaacga agcgctctgt tgtaccatat ccctgtattt gaagtctgac ttctgcgcgc
     7621 cacacatatt atatctacag tatcaaagta tttacacaat cgttgatgga cattaaaacc
     7681 cacaggagta aatcttttct ttacccactg atcaccaata agccatccta agacctttcc
     7741 tgatttcaag atacgatgcg attcaccgat tactttctct agctcttcat agaatctttc
     7801 tgtttctgat gagatgtttc ctatattatc catgtgatcg ttataatcta tgttatcacc
     7861 ataaggtgaa tcaatgaaaa tcatatcaac gctttcattg gaaagtggga ttttccttgc
     7921 atcattttga ataatatctg gtcttgttga aacaatatca taggcaatac acatacgacc
     7981 ttcttccttg catacatcaa gagttgtgcc gctgccagcc atgggatcta ctacaaggtc
     8041 attagggtct gtgtaacgtt tcaccaagtt ataaatgatg aaggctggtg tgacgcccgc
     8101 gtacttgtta ttgcccttaa gagtcttacc atagctttgc ctaggatagt cccataaagt
     8161 tgtggcttcc aatcgaggtt tgtcattaat ttgtgttgct tccatctcag ggactatccc
     8221 tctccaagaa aagccgtttg gatcctctag ataatctaaa agtaattttg tcttattttc
     8281 accgaaggct tttttccaac taatgcccat cttagaaagg atcttttcag cacttttcat
     8341 agacttgatc tcgccatttt tttccacttc cacttgaaat ggatacttgc caccccaggc
     8401 ttcaggactg atatttattc taccatcgga ttttgcattg aaagcaccgt agagaacatg
     8461 gctgtctatg ttaagcaaaa atagcagatc acccttttta acacccaaga ccttatcttc
     8521 atagagttta ttggttgaaa aaagcatacg gtcgaaacac tctttttgag acttttccgt
     8581 gcaggcaaag acgtaacctc taaggctcga agcttgccgg atttcctcca ttcccgcact
     8641 acgagtgctt gacctaatca tttaacaata tcggaagcta atgttgcttt tattgaaaat
     8701 tggatagata cctttgaccc aagggatcta agagtatcat atagaaattg gcttaaaatt
     8761 agatctctat acaattgaag gataagtaat ttcctgagtt acgcttcttg ttgatttgac
     8821 aatatcattt gtcatgtacc atctagctgg cataatatct aacagaacac cgtcacgaaa
     8881 tcctctacgt gtcgaatttc taacctgaat agttttcctt tcaggataaa ttgtttatca
     8941 tcgccataat taacattggc gtggcgcgat ttaaaaaata taaaacacaa ttgttgatgc
     9001 ctttgaagag agtaaccctt gcatctttgt ccctagttaa agacaaaaag tataaggcag
     9061 catcgatttg tatgatatat cacggaatat gtattgcttt ttggttaggt cgataatatc
     9121 tcgacacagg tataaagtac gattgagcaa taatatagaa atgatcgcct gtgctattat
     9181 aagtgaacgg tttactttga cgcttttcca tttatgatgg caagtagtaa aagtgcgcgt
     9241 tgccgcaatc aacaacgcaa actactaatt caggaatatt gttatatgat taggaaataa
     9301 ttcttattcc aaatatgaca gggtgtacca tccacgcaga tttttttgat catcgatgta
     9361 gcgagtgtaa aaaagaatac aacgaaatga aaggtattcc agacaagccg atttcagata
     9421 aaaatggtag ttcatctatt cacaacaatc cgatagtgct taaaaaatat aaggaattca
     9481 cagaggtacg cgcaaaaaaa ttgtatgagg aactaatcat ccaatacctc aaaagttcta
     9541 atgaagcgga agctgctgaa aaagcaagaa gtattatcag gaagcagtgc aagatacgag
     9601 gtatgcccta ctggtcttgg atttaatata ttttaggtta gatatacgat aataattact
     9661 aattttatcc tctctttaca ttggctttag tgtcatacta atgtatctta ttattcataa
     9721 tccagtattt taacgtctga aggtattcag aataagatga aaggatttgg gactataaat
     9781 ttggtcgaat cttttctcga gggattaacg gaacaaatag tcgaatggtg ttactgcaac
     9841 caatttattc tgtattaaag aatatcttat cttatttccg agtaatagtt catacaagtt
     9901 tggtaaaaga cttctcgtta catgtactta actcgagcgt atcattgcag tacatatcgt
     9961 tagtgagatg gtatcgtggc atgacactct atgtctaaat gacagacacg tgcgtatatc
    10021 cttctggtaa cgctgacaat ctgtcgacgt cactaccaag atgacgacga actgacttat
    10081 caaatgcgat ttgtcgtggt attgtgccgg catttgtatt cgttgttagc aacatatcaa
    10141 tatcatatgt ccagcgccta gatagtagat cgggttctat tctaaactaa ggctggctag
    10201 gtctaatgat gatgtaggtg tgcggccgtt gtctggattg gggtatttga acactgagcc
    10261 tcattaacac tggatatctt gagttgtaat ctcctccagt ttagctatta tatatcaacc
    10321 atatttgata aactcacaga ttgcattcct cggaactaca attaaagata ttagaaattc
    10381 tctaccagca ttgttttaac cagttcgagg ttggagtaca caagctagcc aggaaattct
    10441 aggaatacgt ctcttattac atcaatcata ttttgcctat atatagtccc caaagtagtg
    10501 ctacttagaa cctgtaaata tttgaatgtt acaacttttt aatagaatac aataattgaa
    10561 gaaattcaaa gggataggtg tttcactgct caccgaaata atagaaaaga tagaagcaaa
    10621 tcgcggcgat atttcaaggt cgcgctacgt tttgagacta ctataagcat cgttattgac
    10681 aaaggagaca accgaataat gcagggtata catgaagacc tactaagacg tggtctcgat
    10741 aagtcaggcg ccgacattgc atgtcgccgc gccaacaagt taggtatcgt ggacggtagt
    10801 ttttcattcg atgagtagtc atctgttatg gaaaatgcag tatgattgtc ttatccgcta
    10861 atttgataca atgacttctt gggatactga aaaattagtt atattctaga cattatggtt
    10921 caatacaaag caggagcacg aatgcagaca ttcatatgct tgattcgctg tgtcactaac
    10981 atagcttaac actatactgc tcgttacaat aatgcaaaat tca
//
LOCUS       DAOR01000021           12282 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_18652, whole genome shotgun sequence.
ACCESSION   DAOR01000021 DAOR01000000
VERSION     DAOR01000021.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12282)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_18652"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3807 a         2340 c         2451 g         3684 t
ORIGIN      
        1 agatgtgtat aagagacagg tacaacacct aggtattgag ccgccaaagg gtatcttact
       61 tttcggtccc cctagtacgg ctaaaactct attagcaaaa gccatttcga attaaacagg
      121 tgcaagattt tattctattg atggaccaga gttaataacc aaagcaaatt atggtgaacc
      181 agaagaaaag cttagagaga tatttcaaca ggcaaaagaa aatgctcctt caataatttt
      241 tatagatgaa gtcgatagca tagctcctaa cagagagatt acagctacaa accatccaat
      301 acatatagta gtatctcagc tattgactct tatggatggt atgtcctctc gcggacgagt
      361 aatagtaatt ggtagcacca atagaccaga cgcgatagat cctgctttcc gaagaaccgg
      421 aagatttgat agagagattg agattaaggc tccagatagt aagggtagac tggatacact
      481 tcgtatacat acaagaaaga tgcagctagc gaaggatgta tctttggaaa agctagcaga
      541 aatgtgccat ggtttcgttg gagcagactt acaaggatta gccaatgaag ctggcatgca
      601 tgcggtagcg agacagagga tcagtgagga taaagataac tatgttaaca tgaatgttaa
      661 taataatatc aattactcaa ctaagagaaa taataacaaa attgatgata ttactataac
      721 gatggaagat tttatgtatg ccatcaaaac gataaaacct tccgctatga gacagatagc
      781 tccagtacaa acttcagatg ttagattgga agatgtaggt attcctgaaa atataaagca
      841 ggaagtacga gatagtatag aatgaccatt aaggtttcct gaattattta aggctgctgg
      901 tataactcct ccaaaaggaa ttctaattca tggcctgtcg ggtacaggta agactttgtt
      961 ggcgaaggca atagccaatg aatacaaaac caattttatt agcattaagg ggccggaatt
     1021 aattggaaaa tggatcggtg aatctgagaa agccattcga caagtctttg caaaggcaaa
     1081 agaggtttcg ccttgtataa tattttttga tgaatttgat gcaattggag cgtaaatatc
     1141 agatcctgag agaggagact attctaccat ggagagaatg agaggccaat tgctgactga
     1201 aatggatgga tttgaagaat taaaaaatgt agtagtactg cagcaacaaa taatccaaat
     1261 attattcatc cagcgttatt aagacctggc aggtttgaca aagtattata cattccacca
     1321 cctgataagg atgcaagact aaatattcta aagattcata caaaaagaaa aaaattggca
     1381 gaagacgtgg aattggaaca cttggcagat gtctcaaatg gttttgtggg tgcggattta
     1441 caagcactag ttaaagaggc agccatgttc tcgttaagag aatatttgat aaaacatgaa
     1501 gaaaactccg aatttgaaaa taatattgct gagctaaaca ttctccacat gagacacttt
     1561 gagcaagcaa aagaaaaaat aaaccttcgt aagggagtaa aaaaccatgt cgatgcatat
     1621 gtgtagtata taggtggaaa gaaatggtga aagatgtaga catatctaga aaaactcatc
     1681 ctgaatcaga ctccatggat gatgctatag aggtgcctgc agaatattat tggggtgctc
     1741 agacgcagcg atcacttatt catttttcaa ttggggatga tcgtatgcca aatgcgtatg
     1801 gatatataaa aaaaggctgc agcaatagtt aattctgcag aaggtaaact attaaaggat
     1861 aaagcagatc ttattatcaa agtatctgaa gaaatcatca gtggaaaact cgatggtgag
     1921 tttccgcttt acgtatggca gagtggctct ggcactcaga ccaatatgaa cgtcaatgag
     1981 gtaatttcga acagagcgat ccagataatt ggtggagtat taggaacaaa agatccaatt
     2041 catcccaata atcatgtcaa catgtcacaa tcttctaatg atacgtttcc aaccgctatg
     2101 tatgtagcta cagtgttgga gtttgcgaat catcttattc ctagtataca ggcattagtc
     2161 aaggcggtcc aagggaaggc tctggagtgg ccgcatgtaa taaaaatcgg tcgggcgcat
     2221 tttacaagat gccataccac tcacagtagg ccaagaatgg tctggatatg atacacagct
     2281 taaggataca ttagagatcg tcgaacagtc aatgaatgga ctttacaagc ttgcagctgg
     2341 tggtaccgcg gttggaactg ggttaaatgc tccaaaggac tttggacaaa aaattgcatc
     2401 gcagattcca aattgaccgg ccatcctttt gtaactgctg aaaacaaatt tgccgcacaa
     2461 ggagcgcttg atgcaatggt taatgctagc ttcgcattac gtttgttggc tgtagtactt
     2521 atgaaaatag caaacgacat ccgatggtta ggttcaggtc caagaggggg cttgcacgaa
     2581 ctcaaccttc cttcaaacga accaggatct tctataatgc ctgggaaagt caatcccact
     2641 caacaagagg ctatgatcat ggtatgtatt caggttattg gaaatgatac tgcagtagcc
     2701 attgcaggtt cgcaaggcaa ttttgagcta aacgcaatga gaccaatcat cattaagaat
     2761 gtacttcact cctctcgcat tttaggtgat gcgtctgata aatttctaag gtatagtatt
     2821 gaaggaataa cactagataa agagcggatt agcagatatc ttaatgagtc gctgatgctt
     2881 gtgacagttc tgagcccaat tattggctat gataaagcgt catttattgc acacaaggca
     2941 ttaaaagaga aaacgacatt gcgtagtgca tctatatcta gtggatatat tagtgcagaa
     3001 gatttcgata gaatagttgt gccagcaaac atggtaggag atcctgataa ggacttgagt
     3061 accttatcta acacgtaaac aagtttcaag ttttgctata cgaaatgctg ctcttgtaat
     3121 tcttgattat gctctctcgt gttcaaagaa gtgatatcaa acacacatac ccgatactcg
     3181 aaaaaacgaa aaacattgtg tgttgaaatg aaagaatgca atattatcaa ttgcggcttg
     3241 atgtgggcaa atttggaata ctccaattta agagattgta aaatgtttaa gacaatacat
     3301 gtatcggcta ctatgaataa aaaatgtgtt actactatgc tatgtatgaa aatctctctt
     3361 gaaagctaag agcgttattt cttctctgta aaacagtttc ccacgactgg gtatatcttt
     3421 gagtaacatc ttccactcct ccattttctg agaatgccaa aatatactca tattttttac
     3481 cccaattctg ttcataatag taaggatgat ttacagatat tggatatgcc aacgaggaat
     3541 cgatatgaac ccacttctca tctaaaaggg cctcattcca aacatggtcc agaaaatcat
     3601 ggacaatcct gctctttatc cttattccgc tcaagatggc accaaagagg atacaccatt
     3661 cgccacatct tccaatcctt gcctctgcaa tctttaatac ttcattatac ctaggataag
     3721 tcttcgcaaa tccgcatttg ttacaactgt gggtttctac tacgctcagt ttccatgaac
     3781 ttccactagt tgtctgaatc tgcattggta catttccccg accttcatca atacatctct
     3841 cgcaaatggg agcatttggg atccaactca tatactcctt tttgaaccat tccaacagtg
     3901 atttaatttg agcctcttct ttaagttcca gtatacgttc tggaatgaat aatttgacct
     3961 tattcaagat atctgcatct tgatactgtt cggtaatttt tttgtagaat agagcagtgt
     4021 tctttatgag ttcgctaagg ttacgatctg atgtggacat acatcgaaac tcttgtcgta
     4081 gatttgcttg gggaaaatat attattttcg atgaaaaaag ttgttcttgg tccagtctta
     4141 aaaaatttcc cactcgtcct cattgatact cgcgtgttac cgtgaaaatc tacgatagtc
     4201 ttatcgtcag ttctttcaat tttataagcg aaatgagaaa tgcagttacg aattgagtgt
     4261 ggggaccgat aacctatcta cctccagcca attcaaattc cctagcatgc aataattcga
     4321 tatcatgtag gctgctacgc tagtttccat caaacagcaa ctgttagaca tcgttaacgt
     4381 tggtttactg caaagttatt tggatttaca gcggccaatt tcatatagtt tgtcataggt
     4441 acacatgtgt ggcgtaccct cccgggaggg cggcaacctt atttttgtat ttttttatga
     4501 taagttttaa aaagtaatgc agcctatgag cgtatgaatg aggcctcttg tgttttagtt
     4561 tggccttttg aaaaattgta aattgaccta ccccggagac cagggtaggt aaaaaaatga
     4621 tgtattaaac ttaatatcat aaggtagaat tatctgaatt atcctttagt gagtaataat
     4681 gcaaaccagt acaggccgcc caaaaagggc aggatgtaga aaaacaagaa ctgataacga
     4741 tagaatttcg ggtcaaacag aaggaaaagg catcattcaa gactatgtta ataggatgta
     4801 cggccagttt gaagtagatt ccaatacaaa cgaaccgaag tgaatgtcgg attatccctc
     4861 tatcgaactt gtgaccaatc ttgtaacttt ttcatatatg acaatttaat tggttcctgc
     4921 tcacaggaac ttttcttttc agaaatcatc atttatataa atataggtgt ccgaaattgg
     4981 caggtagcat caaaaaccaa gtgaaaattc aattcaaccc ccatatgctt tcgatatctc
     5041 aattcccaca taatggactg ttagattcgg cagtgctttc gttcgctcat caggggagaa
     5101 actaacaatc cttgtaagac tgtacgccaa tacagactgg gaatcggcgt ccctcccggg
     5161 atccaaaggc atccaaaatt gaaagagtag ttttgccatc ttcaccctcg tattatcgta
     5221 aattagtgtt caaccatcaa cttctttatc gtccccatct agtagaatta gtttgaactg
     5281 atagaggata attctaggtc gattcttcct tttgccagta taaaagtttc ttgctctttt
     5341 tctattccaa taaatcgtcg tttcagcctc agggctgcta tgccagttgt agcggcaccc
     5401 atcagtgggt cgaacacgag gtcgtcctgg atggttagtt ttgaaatgat atgctctgcc
     5461 tctatcggtg attgctccca gccatggagc gctttgcttg gtggctgaga ttctatcacg
     5521 tctgcaatag agtccaaaac cctcaagctg ttgcccttta cgaaccagag gagtggtttc
     5581 cacataacgt acacacgttg atattgcaaa agtctagaac taccattatg ttttaccacc
     5641 atttcccacc aatattttaa cccagaattc ttcatgtatt caaagatctg tggtaatgcg
     5701 tagcgtcctg catacatgac tagacttccc ccttccttca acactctaca cgcgagcttt
     5761 cccaacggtt catacattgg cagccattcc ttgtgataag gtggatctgt aaagattaaa
     5821 tcaatactct tgtcttcaat tgtagcagta tctatgtatt gaaaatctcc gtgaatcaac
     5881 tttattctag aagcaatttc tttgccttgt tgagatccac ttgcggcggc ttcttgaacc
     5941 tgcctgatta aatcttcttt ctgttcttgc cttctgatct gattgtacac actggttatt
     6001 cctacagtat ctcttctgag actatttttt tgatctttag tacctctttc gataatcttc
     6061 ttgcctctct cgtatgtagc cgtagacaca ccgatctttt tcgcaattat ttcagatgtc
     6121 ttccctttct cctcacatgg ttcaagggta gcatctatcg atgctacctt tcgctctacc
     6181 tcattattat tatcgccatc ctcttttttg ttggctagtc ctacgatatg cccaccaaaa
     6241 gacatcctcc tcttcgccct ctctttttct atatcttcta atgagtatcc caattcgact
     6301 ctttgaaacc cattcagctg gcgccttcta aggttaacct cgataacaaa tcccttctct
     6361 tctaacgaat ctttgaattc ctttatgtgg aattggaggg gaatatgaag ctccttacaa
     6421 gctcggaatc gatgatggcc atcgagaaca attccctgct tgttggctat tacaggaata
     6481 tgcaatcctt cttccttgat tgatcgcttt aacgaatcga attctacttc agacaacggt
     6541 ggaattagct tcaggtattc tttgctggtt gacagacttg tattactgct gctcttgctg
     6601 atgttgttac tattatttag acgactaaaa ctagtcaagt tcaaatgcgt atagagattc
     6661 attatttaaa aagaactcac atcgaattta gacagaaatg tttttggatt tcgtttatat
     6721 gagttctatc catttttcaa tctggtcact atcaggccat ctagctggag ctggaggtcg
     6781 acaacatatc attttttctt acggtttctc gcgattaatt gtgcaaccga tgtagttcag
     6841 gaaatgttat agattgtcca tcaaaacaaa aaaatttgta ggaatctctc tttgtagtct
     6901 ccttatgcca ttagtacact ctagcagtta cttaaacagc tcttttcgta tgtatccata
     6961 atgaattact cgactcattt ttcatttagc agctcatggg tgagtatgga tgataatttc
     7021 tcttcataat agttcaaatt gcattacgaa attagtggcg ggccatacaa cataagcaag
     7081 agcaattgat gcgtcaggta ttagatctca caccggccag ctttagtcgg acaatacttg
     7141 ctccgacatg agggatacaa cagctaattc aactgttcaa agtattgtat taagcccgga
     7201 atatttacaa gcttaactgc tccactaaat ggagatttac cgcagcaaac aatcaacaat
     7261 ccaaacatct ctggccttgg tataatgtaa tatagacaat ttttgtaatt gcattctgtt
     7321 atgagtacaa tagacgagtt aaaactcaac gacaaaatcg tcaggcttgt gaaaggagac
     7381 ataacagaga gaaacgtgga tgtcatagtc aatgctgcaa actcctactt aaaacatggt
     7441 gcaggcgtgg caggtgcaat tgtgagaaaa ggaggatcaa taatacagca agagagcgat
     7501 aagataggtt atgttctagt tggttctgca gtgattacga cgtcaggaaa attaccatgt
     7561 aatgcagtaa tacatacagt tggtccaagg atgggccaag gaaatgaaga caagaagttg
     7621 agaacagcag ttcaaagctg tctgagactt gctacagaca aagggttcaa aagcatctcg
     7681 atgccagcga taagttcagg aattttcgga tttcccaagg atagatgtgc aaagatacta
     7741 ttagagcaaa caaaaagttt tcttgaaaat aaccataata atacttctct taaagtgatt
     7801 gaattttgta tatttgatgc cgagacccta ggttacttta aaaccgaatt tgataattcg
     7861 agacaatcat tgaagtcttc ctactaggta ttgtcaagat ggttttagac aaaaaatcta
     7921 ttacatcaaa aaaattgtga taatagttgt gtatgccctg tgaatcacca ctggctatta
     7981 tttccaaacc atagggctaa atactataga gcatattgta ggatttgcag gtcaatcatg
     8041 gtttgcaatt aggtttcttt tccttctagt ccccaacttg attttctgag aggcgtaatc
     8101 ttcacgtatt catggccatt cctcttggtt atttcagcaa ttcctttaat ctctactccg
     8161 ctaactctcc aaggatcaac tgatacaaga tcatctacta cgaaagctac tttgttattc
     8221 tgccgtatgt tccggaattt aagacttttt tcgagattcc atcctccaaa gtaaaagtgg
     8281 ctgccatcaa attcgaatgc gactggtact acatgaggtt gaatgtctgg agagacagtt
     8341 gcgattctac ccaatcgttg ttctttgagg tatttcgatt ctttttcact aaattctact
     8401 acattattct cttgtttccc cattagaatt tctccataat gttatatgaa cgaaggttat
     8461 atctaaattc ttagcgatat gggaaatttg gtcatacctt gcaagataat atgataacta
     8521 acaaaatata ggaataagaa aaagtaggtt gtggtataga tttggctaaa ggtaaaggag
     8581 cattctctgc tcgattatca gctggtgttg tcatacttgg cgttatcgcc attattatca
     8641 tcgcgatata ctacaactac aactactatc caatccggcc tttttatcct ccacctccta
     8701 ctgcaggtaa attagataaa tttggaataa aagaaatcta cctatcgaaa cgcggcggcg
     8761 aagaatggta tctcaatact gataatcccc agaacggccc acgaaccggg ggagagggac
     8821 caccgccctc gttcgtgcaa aaaaacaacg atggtagctg gaaagtacaa agtagtgaag
     8881 tgcgttatgg tgtgttcact tcgtcaggtt atcatccaga tctaattact acactcaatc
     8941 aacaagtgtt ggctgcgaaa ggatacatgc aatcatccaa tgattggaaa aacgtagaaa
     9001 tcactgggta ttttaaagta aatagtttca ctgactcgaa acaaaacggt ggaccgcata
     9061 tagagctagt tgcccggggt ggaagaaata caaacgatat tggaacaata gatggattat
     9121 caagacaatg cgaagcaacc acttatcatt caaattcata tgtagatgga agagttaagt
     9181 tcgaaaaaga cttggagcat acgataggat atacaactgg agatcctgag aagcagcatg
     9241 ctacaagtcc attgcttggg aggtggattg gaatcaaagc agtattctat aatttacctg
     9301 atggttcagt aaggttagag caatggatcg atgagaattc gaataataac tggcatcgag
     9361 ttctcaaatt tacagacaat ggggactggg gcggtggaca accagattgt ggtggtacag
     9421 accacactat aattacatgg ggcggtccta ttgcaatatt caggtgggac aacataaacg
     9481 atatggatgt tattgatcta agcgtaagag aaatgcagca gccatcttaa ttcattgtga
     9541 atagagtaca gaagttcagc tgatagaata cttgatgtta gcaacggttc tgctgagaag
     9601 atggaagcct ctttcaatgg tagtgtattt ctttaaggca tctatgttcc gagctaaccg
     9661 taattcatac aaccagaatt cttcgtctaa agatatcgaa taattctatc aaattacagt
     9721 tcggctcctc cggtatttta atcgcatctt gtagcggtta gctcaatcct acaaggagtt
     9781 ataattataa ttataattat gttaattttg tgctaaattt aacgagcgcc ttgtcacttg
     9841 acaaggaata tattatgagc acaaataaaa taataaaagt tagcatgaaa attatattcg
     9901 cgaagtaatt ttaattcttt gaatctaaaa acatttatct aaagagcttt tatattcaaa
     9961 tatttttatc aaccataatt tacctcgttg tttaatgata taacttgata ctttttacta
    10021 ttagggaatc gtgagataag tgtaaacaag tggtacgcag gactgtaaga caggtaacgc
    10081 aatctaaatt caagaaaaca tctccccagg agcgactata cataaaaacg atttctatcc
    10141 atagtatctc acagctggag aacttgaaag aaaatctttc aggtaaggaa cccataatcc
    10201 taattgccag aatagcacca atagtctcaa gagatccaga tgccgcaagc aaattagtga
    10261 atgagctata ttcgacaaac ataaaaaaca gttattcagt ttttcgttta ggagaagaaa
    10321 gatttatggt cgtccccaat aatgtgcaag tagaaggtat tttattgtaa aatagcaaga
    10381 cgaaattagt ctattactag gtatccaata agttccctat cgttgatttt aacgtgtaat
    10441 gtgcagtata tctacaacta gctttttgac cggtcagacc tcaaattcaa gttgtggggg
    10501 tccttgggta aagtctggag aattagaatt tatctggagc ttctacaatt gtatttattc
    10561 tgcagtgcgg gtgatgatat tagaagtgca cgtggataga catcattcta tctccgatgc
    10621 agagttcaag tcagaattag aactatttac taatcatgtt tgctacttat ttaagagcat
    10681 caatacataa agtgtcactt atttaatgac ggataaagca agaccaacag ttccgctaga
    10741 gaaagttaat ttagggatca ttagttcgtt tgctagagga cttaatacca gaatttaaag
    10801 cgagtcgtga tggtgactat agttttggtc cgatagtgaa gttgaacagc acctgttcga
    10861 aataatatat actacatttg gatggctatt tgcatcaata gatatttctc ggcaaagact
    10921 cggtcgtgag agaaggagga ttatttctta tttgcgttcc ttcactcttc cacggcctgt
    10981 ctttcttgga gctatatttc caaccttgcg ccctggcgga gtggttctgc ccacagatga
    11041 ttggcgcccg atatgctgat gtctgcctcc accatgtgga tgatagaccg cggcttgcgc
    11101 gatacctctg acagttggat ataatctccc atgcgcctgc atatatttag ctctgcttcc
    11161 tgcctttaga aatggtttct ccgttttacc tgcacccgca acaattccaa tcattgctct
    11221 acattttgga ttaactataa gaaacttgcc agagggaaac tttatcgtta caccctcaga
    11281 accatgagca aaaacaagag cagaagatcc tgcagcctta attaatttgc cgccgtcacc
    11341 agcgttcctc tcaatattgc aaactgacgt tccgtccgga atggcctcca atgataaaat
    11401 atttcttgca gttgctgtcg ctccaatacc caattcaatc ttctctccga cagcgatacc
    11461 ttctggtgca ggtacatatg aataagtacc attatcaaaa cggattttgg tcagtggggc
    11521 atcccttcca cgttcatgta cgatatcaac aactgagcca gagtgatgtt ctcctaattt
    11581 gaaattcgga tatttggctg gagcaagctt tccgactgta attgctctga attgctttcc
    11641 tccgcgccct cttctgcgga ctaatggtcg ctttcccaaa actgatcaaa ggaaccacca
    11701 aatcaggtga ttttaagttt gtgctaaaat caaggaatat aatgcacata tcgtattctg
    11761 ctgttagtta ttatacatat ttttagcgaa ttggcagttg cagcgaaacg taataaggta
    11821 taaagatgag taagtcctag ttgtcattgt gagccagaat actctatctc taaaggtact
    11881 ggaagcctat actagggatg tgggtcgtgg agtagctcgt attgactatg attctatgga
    11941 ttctttaagc gcatctaccg gtgatgttat tgaaattaga ggcaaacgta gaacggttgc
    12001 caaatgttta ccactttacc cttcagatga aggaaaggga atagttcgag ttgatggttt
    12061 agtaaggaat aatgctggtg tagctatcgg agatactgta gtagtaagga aaatcaaagc
    12121 agttccagca gaaaaagtta tagttgctcc tttggaggca atacccccga ttgacgagcg
    12181 ctatcttgct gatgctcttg aaagtgtacc tctcattaag ggagataatg tcatggttcc
    12241 ctacttcggt ggcaggctta cctttcaagt tattggtgtg ac
//
LOCUS       DAOR01000022           21965 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_5130, whole genome shotgun sequence.
ACCESSION   DAOR01000022 DAOR01000000
VERSION     DAOR01000022.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 21965)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21965)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21965
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_5130"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         6623 a         4328 c         3962 g         7052 t
ORIGIN      
        1 tgttaattac gcaagagatc aaatatactc aatccaactg gtatattttg taaccttgaa
       61 catgtagcat caccatcggt tgagtatcac ataaggtttc tacatgctat tggatatacg
      121 ccacaaatgg aagacaaatc aaataagtta ttgtccatgg aaacacaact aaaatggcta
      181 agggaaatag ggtttgtaga tgtggattgc tattggaagt ggtttgaaat ggctctttta
      241 attgggtata aagcatagca aaatctggac ctcttattga gagaaattat agtaattaca
      301 gccgatttaa ttttggataa gatagttttg tagattcttt ctccagctca tcaacctcta
      361 tgtgcatatc tgactcttta ggatgttatg acaagattga aatttcgctt ccaataagaa
      421 ttcatgttca atgctaaagc cacaattcct gttttgtttg ctgaattttg tctttaattc
      481 tgtcttccag ctctgagaat tcattcatat agaatcaaca tatggatcct ttatcgatat
      541 attcattgta ggacttcagg aagagggagc ctgattttat tgtctgcgac attacaacac
      601 aatctgcaga atattaccgt cgtcagatga aattattgat gattagtgct ttgctttctt
      661 tgcgtcggta gtactccttc aagtatctat agatatccta aggtaaagta actgatttaa
      721 gttatgaacg tgatgataat gcatgtgcga cttcatagcc tatgaaatca aagataagtg
      781 gcgattcaag cacaagattg cagaagagga agacttagaa gaagaacctg aacttgagaa
      841 ggtacaagaa caacctatta cgatgaccca ataaacaaga agaccgtagt agaattctgt
      901 ccgactggcc ttaatctcat cggtgtatac tcttgctgct tgcaatggcc catagcttca
      961 tatttttttc tttttagtac ggatgtgatt tacaggaaat gctggaaatc ttaaggtgat
     1021 tctaaagtgc taatatccta aacaaacaag tttatctatc tcttgacaat accactgctg
     1081 ataatgacct ggcatggaaa tcccacatct accattttat tatcctcttt attattgaca
     1141 ccattaacga ttctatatgc ttatgtgtcc ctataaaaat aagatactcc aactcccaga
     1201 agaatcccac ctattacatc caatggaata ggaccaccaa catatactct cgatagacat
     1261 acaagacctg ctcttctcgc agttgatcat gttgttgtta tttttggggg tgactggtaa
     1321 tagcggtttg tttaagatca taattagaga aaattttctg cttctccaat tccattcttc
     1381 ttcctttact aactttgaat ttgggtttgt atcttccaag taatatcgaa ttgcctacta
     1441 ccgttacaga gctcattgcc attgctaaac cagcaagcat aggaagccat ccaaacatat
     1501 tcaaaccaaa caacggcact aacgcccctg ctgccaccgg tattaaaccc gcattgtata
     1561 caaaagccca gaaaagattt tgctttatct tagatactgt cttttttcct aaatcaagtg
     1621 ctataggaac atccattaga tcatctttta tcaggataat tcctccggtc tccttggcaa
     1681 catctgttcc agaacctatt gcaattccta gatcagcttc tgccaatgct ggcgcatcat
     1741 ttatgccatc tcccaccatt gccacgacct ttttttcttc atctcttagc tttgagatta
     1801 cctgttgttt ttgttgcggt aatacttgag caataaccct actaattccg agtttggatg
     1861 caactgcttt agctgttctt tcattatctc ctgtcaacat tgctacttct attcccattg
     1921 aattagtaag tatatcaatc gcatcctttg cattcttttt tatagtatct gcaagagcaa
     1981 ttattcctgc aagtttattg tcgattgcaa caagagtacc tgttttgcct tgaatttcaa
     2041 gctgtgtaag agttgtgtct acagtttcag taactggaat attattatca tccattagtt
     2101 ttctatttcc gataagaatt gtatgatctg catatgttgc tctaagacca tgacctgata
     2161 ctgcttcaaa tgaggtcgga ttagagatta ccggaattcc tttttcttta gcattattaa
     2221 caactgcttg acctaaagga tgttctgagc cagattctgc aattgcagca agtctaagca
     2281 attcattttc acctattccc gacaaatcca atatgtcagt tacagacggc ttaccttttg
     2341 tcaaagttcc agttttatca aatactattg tctttacttt ctttgctatt tccaaatatt
     2401 ctccgccctt aaacaagatt ccattttcag ctcctcttcc tgctcccatc atgagagcag
     2461 caggcgttgc aatacctaag gcacagggac acgcaattat tactattgat acaaatgcta
     2521 aaagtgaaaa tgtcagtcct atatccccta caaagtacca tccgaggcct acgcctgttg
     2581 ctattgctaa aactgcaggt acaaaatatt ttgcaacttg gtctactaac ctttgcatct
     2641 gtgcctttcc tgttcttgcc tcttcaacta acgttattat ctgcgaaagt acagtatctt
     2701 gtccaacttt tgaagctttg acttttagta atccactctt gttaatggtt gcgcctataa
     2761 cctcatctcc cttttcctta tctacaggta tgctttcccc agtcatagct gattcgtcta
     2821 tagacgagga gccttctatc acgatgccat ctgttggtat tctttctcca ggtcttatga
     2881 tcataacttc accttcctgc acctgctcta ctgggatttc tacctcttca cctccttccc
     2941 tcatgacttt ggtcattctt ggttgaagat caagtagttt ccttacagca tcggaagctt
     3001 tttctttagt ccttgtttca agcaatcttc caattaatat taaggtgata ataattgctg
     3061 cagattcaaa ataaactgat ttaaatggaa aatatcctgg agctattgta actattgcac
     3121 tatacaaata agcagctgat gttccaatcg caataagtgt gtccatatta gatgctttag
     3181 ccttaattcc atcccataat cccctataga agcgccaccc tatccagaat tgtaaagggg
     3241 tagctaatgc tagtgttata tagttcccat aatgcataac tgcaatagga aacagaccac
     3301 cactaaactg tgcaggaaac atgtgaggta agctaaaaat tactatgggt attgttagtg
     3361 ctatgcttat agcgacatat ttcttgagct tttttagttc cttttcaggc tgaagaaatt
     3421 cgtctaagca ctgcttactg caaaagtaat attctcttcc atctttatta taccgtattg
     3481 catcaggctt ttcttctaca aacataccac aaacaggatc tttcgccatc tcttgtaatc
     3541 ttgtattata gctagacgaa cagatgtata agcctaggtg tttacaattg taaaatatga
     3601 tggtatttca atttatagac acatccagtc agcaaagaca aaatatccct ttttttgtgt
     3661 ttcagaaacc attttttagg tacatttaag tacagtaaga agattattat atttaccaac
     3721 attagtagga ataaaacacc tttacttctt aatttttact tgtggtacgc ttctgtaaat
     3781 gtgagtaata taatgagtat aactatcgca ttctaaaaat tgacaattag gtaactgatc
     3841 ctcatctatt atatgcagta taattatatt cttctcagag tattaaatga atacaaaaac
     3901 aaaggcattc accatatttg ttattgtggc ggcaggacta gcgctcataa cagcatctag
     3961 tttctctatt gattctgtgc tagctgtaaa gacaagcagt tcccataaaa gcaagagcag
     4021 cagcggcaca tcaacttcat catccagtaa ggaattgaaa aatctcattg catgtgaaac
     4081 tacttcagct aaaggagcag gaggtcttag tcagaatcaa gttctgaatt gctatagcca
     4141 agcctttgct ggaaacacaa cagccacgac taccaactct acagtcccaa ccgctactgg
     4201 gggtaatagc agctctacta ataatgttgg tgtgggtgct ggtagtacta gcggtagcgg
     4261 cagcagccag agctcttcta gcagtcactc acatcatcat agctcaggta gttcaagcag
     4321 atgatatccg cttgtcttat atcccaatag caaaaatcgc tattattttt ttttatatgg
     4381 gtgatcgata agtgagatta tgtgagataa cttattgaat cgactagatt tcttgcatac
     4441 tattaccaat atacaagata tatgcttcta ctatattaac agtcattttt gataacgttg
     4501 ctaataacta gtaatgactt cgtattatta ctagaattat atatcgaata acttcaatac
     4561 gaatcatatg taaacttttt attatgaata ttttctgtac cgattttaaa ttattttcct
     4621 tctaatttgg tattgctagt tgtatgtgtg tgctatagtg gatcaactaa taatacttat
     4681 tcttgctccg atcttctttg caataagttt tgtttattcc agtgtaggtt ttgcaggagg
     4741 tagtagttat attgcgatac ttgttcttgc tggtgtaagc ctatatactg tgcctccaat
     4801 atcgctggcc ttgaacataa ttgctgccag tatggccttt atcaattatg caagagccag
     4861 atacttttca ctaaaatttt cacttccatt tctatcctca ctacctttcg ttttcttcgc
     4921 tggactactt gtacttccag aaaggagtct tgccctcata tttgtaatag cactatatgc
     4981 cgcttcagct gcactccttg cttcaagtag taagattaga acccaaccga ataaaatcag
     5041 aaaattgaat cttagtaaca cgaaacttgc actcatcgca gtcccagtag gagctgtgtt
     5101 gggtgttgtt gctggtttag taggaattgg aggcggcata tcaccacttc ttatgcttag
     5161 tggattagca gatccaaaaa gggccgcagc tacagcccag cttctttata gtaacgaact
     5221 ccattattgg tttgctagca cattcaataa gtaggtcaat cgatgtctcc cttctagcac
     5281 cgttagctgt ggttgtgttg gcaggtggat ttataggatc caaatttggg gcatttaaac
     5341 ttgaccatga taaaataata ataatagttg cagcagtagt agcagttgca ggaattgaac
     5401 taaccactaa actattggcc tagtctgtag gagcctataa ggcagaaaag cgaagcagta
     5461 aaataataat aaaatcttcc attccagata atactaccaa gaataatctt aagttcttgt
     5521 gtgagataat tctttgatgt aatatctaaa tttgcaaagc aaattgaaat tatgacactg
     5581 aagaacgtca tagcaaagcc cctaccaggg attcgagaag cgtattcata atttatgtcg
     5641 acctcttatg aagaaaaata ggaaatggtg gtttcagatt agagaggagc gtaccataga
     5701 cattgaaact gagagaatgt aaaaattacc aggctggaag aaaatgaaga cctgcagctg
     5761 aaggacatta tattacctgg gatcgagaac aagggtgtcg ctataacttt ggcttgcttc
     5821 cattctatga aaacagtaga tgctgttatt ctcgcgcttg aaggtccatt cttgtaccaa
     5881 ttttcgacat tacatggttg tacacggcat gtagacccat taaataccaa cgtatatatg
     5941 atgcggatgc taataatagt atagatgata tataatggta gacaataaat ctaaagacga
     6001 cataaccact tcaacaaggt caactgaagc aggtaactta tatgatacag taagacaaaa
     6061 ctacattaga atagtagacg agtttacaaa agctcaacca caatatatac aatcggtctc
     6121 taatgtacaa cttgattata ttgccgcaac aaagaatact atccagaata tggtttcagc
     6181 tcaaaagttg ttagcgagta acggcaacgt tcctatgata acaccaccat acacagatga
     6241 atttgtaaag caatcaaacg agatcaccga gaacatagtc agagcagttc gtatcaacaa
     6301 ccagctgaca gtcaacgcga taaatgcagc atcagaaaac tttagaaccc ttaatcgaac
     6361 aattgagtcc gtgacagaat ttaacaccag cgccgcaaag gcttggaatt cattcctcac
     6421 agttcaacaa caacagttcc tccacaaaca ataatttttt ttgtttccat ttgtctttat
     6481 ttaaatgttt atagcatata ttttttgatt tagcctaaaa tatggaatgg agtatatcct
     6541 tcttttcata tttggcatat tttgaaagtt gctctgttat ttatacagta caactttatc
     6601 tgtggaaggg tttgagggca acctttttat ggacagtgca ataccggcac cgaaatggta
     6661 aacacttatc tacacctggg atgttaccgt cttgaggttt catcagcact aaagtgaatt
     6721 gaaagaacca cgaatccgat cttcctaagg ttgtacatta tgccaagcat ttttccacgc
     6781 aaaccgtact tttggttaag aagttgatta gcctgttgtg attcataatc tttagcgatc
     6841 tggatcgtac catcaatcca ttcgcctttc ggccgccctc taatattaca tggcattgtg
     6901 cgcacatgcg gattgttccg cacacgtttc actttgcctg aattcttatc tgtcctaaca
     6961 taaagtaccc cgttgtcctg aacaaaccaa actggcgtat gtactgcttg gccacttttc
     7021 ttgtaggttt caagattaat gtacttttga ttcaaaaatt gcgctagcgc gtctctactc
     7081 aattctattt ctctcttgta tgtcctgcaa cattctaata tggtatcgct tttcataatc
     7141 ctttctcttt gtgaaatgaa gatgttcgtt attgaaggaa gtgattcata tctcagatgg
     7201 aatcatgtga ttgaagccat cacttattgt actaaattga ataatttgca gtattttact
     7261 ctattagtgt aaatttattc attagcgtct acttgtagaa cccaaaatta ttgatcggcc
     7321 ttatcctcta cccattgcag cctaaaggat aaggccacag aaattggtgg ttggctttct
     7381 gtattactat atagctattt caaacaaata aagttagttt gttctaatac attgattatt
     7441 tagtatattg ttgtacatta tgccatccat taatctgaca taattgttat actgtgtaac
     7501 tctaatgaca agatctcgta cttagagaga ttctaatttt tcgagtacac cttaaggata
     7561 attaagtcat tattaagact ttggctgctg agaagaatcg agttttagga taatgttgag
     7621 tactcactcc acaatggttg caaaagcgaa catatcctta atctttaatt aacttctttg
     7681 gtccatttat aatcctgatt attctgtctt cttttcttag ttgcctccgc ctttcttagt
     7741 gaatagaaaa gatagttata ttagcgacgg aagggaatta gtcaatgttc agagacacga
     7801 aagcaatttt ccggtttttc tgtagacaat ttgcaaaaag cgaagttcta tggccagaca
     7861 ctcgggctgg aggtttccga atcgtacggt ctgttggaac tccatatcgc tggcgggagg
     7921 aacatattga tatatcccaa ggccaaccat actccagcac gtttaccatc cttaattttc
     7981 ctgtagacaa tcttgagcag gctatagacg accttgctaa gcgtggcgtg cgtttcgaga
     8041 tctacaatga aggcaacctc aagacagacg aaaaaggtat ttctctcagc ggcgagggtc
     8101 cgaaggtcgc gtggtttaaa gactccgccg gcaatgtgtt gtctgtgctt gaagagaagg
     8161 aatgaattag gagtgaaatg ggcgaggcgt catgcgtgtg cagtggagga tgtttatcat
     8221 ttcctggtag cagctctcaa ttagctttat caggtatttt cagctttaaa tcataatcgc
     8281 ccgttttcat actctcgctc tctatgtctc aagaccattc ttctgcaatt tcatgccatc
     8341 actttttgtt gttctttcta atagattctg catcaaaaac ggccctatcg aagccaaaat
     8401 atcctaatac agtttctgca gcctcaagca gcattatcga aactttatga ttgcagttgt
     8461 aacaccacag cgtatttttt cctctttatg tggaattaat ttcgtaggta ttatgtagcg
     8521 tttgcttttc tagccgtagg tgaaaccctt agggccgttc cacagcacgg acaaaaggtg
     8581 ccatcatgaa ataagtaaat ttcacacctt ccacagtatt tcttacaagc tcataatggc
     8641 tcttaccgac aataattttg gaatagagcc tctcacatag atttctacaa gccataggaa
     8701 cttgtaagaa aaacttggac gatctgtatt aatgtagcaa agtcctctta atattttccc
     8761 atgtgcagtc ttgatttcga acctcttgca aaaatgccac ctcagtatcc aagttttgtt
     8821 cacgaatcat gtaacttgca taagtaaatg gaaaaggaat atccgattca tagtatattt
     8881 cggcattttg ttttgatccc attggggggt tcatatttat attaataata tggaatccag
     8941 caaactccat atgttgacgg attttctctt gatcaagtgt cttttctgta tggttataga
     9001 ctattaacat tctatactgt ttagtcatga tcattcgcct ccgtcaattc aaataaagga
     9061 gaaaatgcat tcttcacaaa gataacaaat tctgtcaaag tcggagtata atgtcgttat
     9121 gtcatggctg cgaggacatt caatatcttg gtcggctgct agattattac tctcttcttt
     9181 cttttccata atatgttgag taggcttttc ttccacctct gtcagtaatt tactcttact
     9241 tctgcttttg gacatgacag ctacgtgtaa ttcattgcta ctaatataga atgaaatact
     9301 aaagggacag ggagggacta gtaaggtata ttccattaaa tcttgccaga agatactgtg
     9361 ggattttcag taaaagccca tatcgccaat gttaaatact cctcgatttc ttcatactct
     9421 gactagccat gagtctgcaa cttaggtaaa ggtgtgagac gcggtaagcg acatgcatta
     9481 acaaatcctg atcattttag tggtctttgc cttcagcgcc attatttata aataaaaaaa
     9541 taatagcacc gacattattg ggaatataag gctctcttgt gctaactcat tatcttcaaa
     9601 taggcactat cttcgttaaa ggcctgttct aggaaagaca taactgcttg tttcagctga
     9661 ttttcgtctg gcatcattga ggatgtaaat tctcctacta ctgttacccc cgttttgttt
     9721 ccgtttggaa tatagtagtt gaagaacgag cctgcgattg ctccttccag taattctata
     9781 gctaccccca atgggtagaa tctagtgact tttatctcag tctttactgt ctgacccttc
     9841 atatcgcttt cccagctttg aattgcagcg ttttcgctta cactttcagt cttgtaattc
     9901 ttaccacttg gatgaatctt tgctgcgtcc ttcgcatgca cttcacttaa tttccaaact
     9961 ttctctagtg gagctccaaa ctcgcttcct tcatcaacta tgaataccat taactctatt
    10021 agttaattaa tttagcaagt atataaccat atcattgaaa tatgctactg tttggaaggt
    10081 ggactcacat cagaaacttt caccaactga ttggaaccct tagccagctg ccgcgatggc
    10141 gtcagcatga gaagttcttt agtagtggct ttatctatca aagcagaggg atttgttaca
    10201 ataatgaact gaattctcat tttctcccct tattatagat ctaaagtatt ctagtactct
    10261 ttgtgtatgg tatatagtat tgcgtttcac tgcctattgc atcaggagtt tgtatggatc
    10321 caaagtttgc tagctcattt aatgtgttag caagagcatt gtaaaaacct ttatggaatt
    10381 cattacctac atgacattta ggatgtcctt cgattaattt gtggttgcga ttttgacaat
    10441 ggccttctat agtttttttc ttaaggttga caaccaacca tgacaatgaa atcaaaccta
    10501 gttcgtcagg gtaaccggag tacaaattca tattcccaga ttttaattta gataaactcg
    10561 gggttttttg gatcttcttt tcttattact tcagaattca caaatcggat tttgtactat
    10621 ggctagtatg gatactctga cggatacaat agcactagtg agttgagcgt ggtcttgtcg
    10681 gttcaacggc aaaactgcaa tccgtcaggg ttcttaaaaa ccattatttc aggaaaagga
    10741 ttacaatttc aaagtttctt gtcttaactc actgttatat ttttctacca actttactcc
    10801 tgcccatagc gtctttcgtt ctccagacga ctcccttctt tcacctaact tccactttcc
    10861 ttccttcctt tactgacata ggtatcgagg atttgttaaa agatcattca acgagatgtg
    10921 gaatcatatc gtactgtatg tatacaccgg tcagtcaacc attcgtcttc tgcatctatt
    10981 gggttacttg tattacggct catgcagatc ttatcataaa atcattaaga attctcctct
    11041 cttaacaaaa caggagagta tcgttgagtg ttgaagttag gatcattggc ttcattaaag
    11101 gaaataatat ggacgcaatt tggaaatttc tttaaataac cgtaagccaa aaatattaag
    11161 tgagtcgaat ttgagcatca ataataacaa acaattcttt accaatcaat tagtatggct
    11221 aggaatatcc tttggaatta gtcttacgat ctccatgtta ttgccatttc caatatcctt
    11281 agcagttata tttggagtat tcatattgtt aaatttgtat atgcgaagga taatgatgcg
    11341 aaaaacgggc agtatgacaa gatcaatgtt cggcagcggt gacaattctt cattgaagta
    11401 ttactgtatg agctgtggaa cacagcacaa acaagctgca tgtcctaaat gcggttcaaa
    11461 gatgaaaaga gttggatctt aaattgttag tatttattac ttagcctcag gactacctaa
    11521 agtccctttg ttaaatcgaa tgtccataca aggatataat aattgattgc tgtttatagc
    11581 ctgacgagaa tggtaagaag ggtagacaat gatatacaaa aactggggag agtaggattt
    11641 gtcagttgct tatccccatg tcttgtgatt ttgtctgtat cattgatgtt tttttcgacc
    11701 tcagccattt ctacaatatg tactgctcaa ctgcaataag caatcgcaca gactactact
    11761 gtcactacca caaaaccatc cagtagtaat tcattcctaa catacgaaaa taattctacc
    11821 ctaggaatta agatacagta tccagctaat tgggaaagca cagcatccat taattaagtg
    11881 ttgatctaac tttactgcat gttctatgaa gtgccatgat tgaaatacta gaacgaaagt
    11941 aaccagtcca tagataagct gcatctttct gattggtgtc tttgtaaagc cttccaaacc
    12001 cacgctcaaa atctaatgaa gcaccgagta tcccatgggc gactggaaac tgtaagacga
    12061 acttttgtat gacttgaacc atgtgctcta agatgtgaaa tgattgaaca gctacagcta
    12121 cggtcaaaat cacgtacggt ttgaaaagaa aattactact catcaatacc ctctcgcatt
    12181 gcggatagta ttaataatga tacatggtac gtcacttgta ttattaattc tttcattgta
    12241 gttggatgat aaaggcaatg atacccaaat gttccaatca tgtttggctt aatcccatct
    12301 ctaattatgt aatctgtctg cctatggctg attaaagtac atgttcccaa tgaaataatt
    12361 cagatataga tttgaaatcc atgcagttcg gcctcccccc ttataatgtt atatatacta
    12421 cattaatcgt taagtggaat ctgcagggta aagctattct tttatcttta tatacaaaag
    12481 aaatagaaga tattgaaact tattctgtta gcatatctag acgtcgccat ttcttaaact
    12541 taaaagaatg tagacgtctt aaatagaaag atcgaggctg gtagatctgt tcaatctaat
    12601 ttccttaatt ctgttacaca tatcgcaatg ctccggacat cctttgtgaa gacaaacact
    12661 ttgcatagag ttacaaccaa aacaccaaat ttgcttagaa gatggggaca ttcttatcac
    12721 actatccttg catgtatatt aacaaaatga attttgaata ctgaccggcg tgttggcata
    12781 ttttcacatg tctaaaagtg gaaagccata atgtgtacaa cattcgtaaa gatttatctc
    12841 tactatattg cggaacaaaa ttagagggtt aagttaagtc ttgatgttat gtattagata
    12901 tactttcaaa gttagaacag atgtagaagg cctgtgggtt ggtgaaaatt aaccatacta
    12961 ttctagtgat taatactaaa agctgaagtt atggattatt tttccgtagt agatatatta
    13021 gtgtagagtg attatatatt actgtaactg cagtggcaat ggctgctcta aacgacacga
    13081 aaagaattgt gcgctctagc cgtgtggact ttaaagtata catcttaaca attagggcag
    13141 atagacattt tttttatttt gacggattgc atcatctttg ttttgtggga agaggggtag
    13201 gaaggcgacg tactaatgtg aaagcgttca atccagcatt tgcataccat tttgattcag
    13261 aggtaattga tcattgatac aaatcagtcg ccacgactca caccagccac acgaatatct
    13321 gcgagacaca ttagacttaa gatatcgaga atgtagggct tgtaattcaa ggagccaatt
    13381 tacctgtata agatgtgggt tttgttatag ctgccactgg aagaaagaag agctggagag
    13441 aattccaccc tatttcatgt tgggagatag atggtatgaa gagatcacat ccagataagc
    13501 acaaacgctc gacttcaaaa aaaaggaaca tatatcatga tgagcaggaa aggaaacaga
    13561 gaaaagaaag aaaagatact cttaattatc taactttggc attgaaggat ttaaactact
    13621 gtttgaatac attaaagata aacaaggaca ctcaagttcg tcaaaagatc aatgaatttt
    13681 tcgaacaagc catcactcca gaaggtatca gtacgtatgg agaacgcatt gttactatga
    13741 atgtaatagc agacttctat ttctcgtcta ctagcaggta cattttttct cagccagaat
    13801 tatatgattt tttgactctt gctacgcaag gattaccaac tgaacaaaag agagccagag
    13861 caatcgaaag ggtattcatg atttatagag atattatgga aaattatcaa acctcaaacg
    13921 attccctcag gtttaactac cccaagatta ttgttgatgt tatagcgatc ctacatgctg
    13981 gggaagatac tagtgggaaa agagtaggag ttaatgctct agatgaatac tatgactcta
    14041 ggaagataaa ggaatcttca gaagtgacat cagcatctgc tgcggctgtg cagataaatc
    14101 caaccctaac agcagatttc cccgcagatc agttcgaaaa agaccaaata cgagaggaac
    14161 agctcattga aacaatcgtt cagattacag gaagaaaaaa taaggacata agagaatcgc
    14221 ttgctaagtt gcacagttca gacattaaga agataagtga tctctgtgcc aattataata
    14281 agatagaaaa atattgtaag cttgcaacaa acccaaacga atttagagat gaacttgaaa
    14341 aagaacttgg cagaaaactc acagacaatc aagttcaaag ggctactaca tctataaaaa
    14401 aggtgagaaa atatgttgaa aatctacttg acggaaaatt tgttcctcat ggctcacacg
    14461 gcataaacca catcaaacac aatctagaat acggatacca ggctatggat ctaattgaac
    14521 acaagaggtg aagctccaaa taaaattaaa agtattatat agcacctatc gcagcggccc
    14581 ctctacgact catacgacac ctcaagagat tctaaaccta ttggggatca taattagagt
    14641 ccatagttcg actcaaacaa atctgatctg ttgaaacagg tatggggcta aggctctctt
    14701 ctactcgcaa aaaaggttaa aacaaaatca acaattgtca tcgagtttag tttattttct
    14761 taataactta ccagcttttg ttgctattcg gtcggtcatc tgtgatagag tagcattacc
    14821 acctaggtcg tatgtaacgt acttattttc gttaatgaca tcttcggtag ctttaaagat
    14881 tgccttagaa atgtgttttt ctccaaggta ttcaaccatc catgcgccag ataaaatagt
    14941 tgcgacagga tttaccatgt ttttaccagc atatttagga gcgctaccat gtgctggttc
    15001 aaacattgcg taatggtctc catagttacc agaatagacg gttcctatag atcctacgtg
    15061 ccccgatgca cattctgaaa ttatatccat gaataaatta gtgctaagca agacactgtt
    15121 gttgaatctc tctggatttt ttacaagctg ttgtgtcata ttgtcaatat agtattcttc
    15181 taattcgata gttttgaatt ctttttgagt cttttctact gctgcccaga atattccatc
    15241 agtttctttt aggatattcc tttttgtaat tgcaaaaact ttcttaaagc ctgcatcatt
    15301 tgcgattcga aatccctttt tagtaactct ttcacaccct tttcttgtta tcttccgaat
    15361 tgcaatggct gaatcatcac tggttttgaa ttcgatccca gcgtacaagc cctcagttgc
    15421 ctcacgtaca cagataaagt ataaccgtct ttcagcgttt ttgtaagttt tgataggcct
    15481 gacattagca tataattcaa atttttgtct aatactcact gcaacgctgc gtggagcatt
    15541 tgggtttgga atggtagtag ttggtccctt aaagcaagcg tcactatctt ctagtagctt
    15601 ccaaacatct tgggaaatat aggaagtgcc acctactttt tgccaccact cagatccagc
    15661 atcacatgga attaattcta tcttagtatt acattctctc agaaccttta tcatagcctc
    15721 tacaagttct ggtccggtac catcgcctcg aataattgca gcagttttct ttgacaattg
    15781 aaatcaatta acgggcaaaa tcctctctat ttatatgtat ttggagatta attccaatct
    15841 tacaaccaaa ttgttagtga acggaggtat ccttgatgga gaaatactgg caaaccacta
    15901 cctgtaatca cgcctccggg atttgaaaag ttttttgaaa aattagttgt accaataact
    15961 gatccttgag tccatcctag atatcgccta agtcaagcac ttagtgcgaa atctgaatcg
    16021 agccattata tgattgaaag aataccacta agtagccgca tgattctttg atctgtttaa
    16081 ggtattaatg gtaaatattc aaaacaacaa ctttgtatga acgccatatt tttacggagc
    16141 ttgaatgaga tctaatttag aatattgtat gagaataact taaattcatg ctatgaataa
    16201 cccatatgat atagaggtgg tttgaatgca aacaacatca tcctcattac agcagcaaga
    16261 aaagcaagat gtttctcata tcaaaaatat ccttgataat aaatcactta cttttgctga
    16321 aaaggtaata agtataaaga acgttgaaac gcgcaataag aggtctcaaa taatatctat
    16381 ggccggagat atcgttattg tagatgtgga ccttgcaatg gcacaggata gcacaggccc
    16441 tttggcgata cggtcgctta atgaaatggg tatcgacaag ctccacgatc cttcaaaggc
    16501 cttgctagtt atcgaccata catttcctgc ggcagatgaa aaagtagcga atttacatgc
    16561 tctgataaga gattttgtct caaaacacaa ctgtatgaaa attgagggct caatctccca
    16621 tcagcacatc cttgaatatc ttgcgactcc tggtatgatg atattaggag cagactcaca
    16681 cacatgtcaa gctggatgtg tgtccgcttt tgccaccgga attggaagta cagaaattgc
    16741 agcagtctgg gccacaggtc agttatggtt gcgagtgcca gaaaccataa agattaacct
    16801 atctgggact tttaataaag gcgtatttgc acgagactta attttagatt atatcggaaa
    16861 agtaggcgaa gatggagcaa actacaaggc attagaatgg aaattttcaa atgattatgt
    16921 taagagacaa tttagcatgg attccagagc ttgtataagc aatgcgtcaa tggaatgtgg
    16981 cgccaagatc tctgtattcc caaccgatga gattacaaca aaatatttgg acgaaaatcc
    17041 tagaattaac aacaatatgg gaggaaaaat cgaaatccaa ccagggactt ctgccaagta
    17101 taaagatgaa tttgagatag aatgtgacaa gatagaacct caagtttctg gtccagataa
    17161 tattgataag gtacattcaa ttgaggagtt ggcaaatgtg gaaatcgatc aagcatttat
    17221 aggctcttct acaaacggaa ggatagaaga tcttgagata gcagcacata tgttaaaggg
    17281 cagaaaagtt cataaaaata cacgatgtgt agttactcct gcatctgtca aagtgtatga
    17341 agatgcgatt agacgaggat acgtagaaat ttatctagaa tcaggagcag tttttaccaa
    17401 tgccacatgt ggagcgtgtg ttggtactca tcttggtgcg ttaggagaaa acgaaatctg
    17461 catatcttca tcttcaagga actttgttgg aagaatggga gcactttcat caaaagtata
    17521 cctagcctcc cctgcaacag tcgcagcctc tgcaattgaa ggtaaaattg ctgatccgag
    17581 aaggtactta tgactatgaa tgatcagcca attattattg ctagcatttc gaaaggaaga
    17641 gcttgggtat ttggagataa tattaacaca gacataataa taccttttag attcaagagt
    17701 aggactaacg atccgtatga aatggcaaag tatgctatgt acggatttga tcctgatttt
    17761 cacaagaaga tctccaaggg tgacattgtg atagcaggcc gaaactttgg agggggatct
    17821 agtagagaac aggctccggt agcgttaaaa tatgcaggaa tttcggcagt tatagctgaa
    17881 agttttgcgc gtatcttcta taggaatgcc tttacaattg gattgcccgc acttgaaata
    17941 ccagaaataa aagggaaagt gaatcaggga gatgagatta aaatagacat aaccaaattt
    18001 accgcagaaa atattagaac caaagagact tttcacgcta gcaaagtccc agaattcatg
    18061 cggcagatgt tgttggaagg cggattagtt gaatactaca aaaagtataa acaatttcca
    18121 tggatccaaa gctagaactt ttacaatcgg tccctttttt tcttgttgta ttgtgttaga
    18181 ggataagtta gatagtcata cagaagcaga aatatcgagg attaaatttt aagctgtctg
    18241 tgattattgt tcaagtctgt caggtagaac ccaaatccga ctaaactgcg atatcttcta
    18301 aaattagtct agaaaattgt cgtcaagcaa tattttattt ctggaataat caatatctac
    18361 agaaatgatg acagggatat cctttgacat cttggcttgc tccaagatat ctggaaattt
    18421 cactgttgat tttactgcat aaccaatagc gccgaagctg cttgccagct ttacaaaatc
    18481 gggattgtta aattcggtga aggccttctt gccaaattcg tcaatttgct ttaaggcaat
    18541 cacgccgaat tccctatcac accagatcac aattattaca ggcaatttga ggcgaacagc
    18601 tgtctcaatc tcttgaacat tcattagaaa acctccatct ccacacatcg caacaacctt
    18661 tctctcagga aaagcaagct gcgccgcaat tgcgccggga agtgcaaatc ccatcgaagc
    18721 aaatccattc gatataagac acgtatttgg atcgtatgta tagtaggtct ttgctatcca
    18781 cagcttgtga gcacctacgt cagaaattag aatatccctg tcatctagtg accttctaac
    18841 atccactatt agtttttcag gcttgatggg gtaactcgga tcatttgcat atctcagaac
    18901 tcgctctaca agctcttttt taattctctt gaaagtagcc ggaacatcgt gacatgggaa
    18961 gcattttaga ttaggatttt gatttttttc tttttctaac tcggttaata tagcatttat
    19021 tgtcaattct atatctgctg caatctcgac agtcggtggg taatacgtat ccacctccgc
    19081 tggcgtaaaa tccatgtgaa taattttttt agtgatatcg ccattccaaa atcttggact
    19141 gtattctacc aagtcataac ctattgctat gaccaggtcc acttccttca tggctattag
    19201 tgcgtgatct gcagctttga taccaattgt ttgaagatgt ctttcccatc tatcagagat
    19261 gacacccttt cccatgaagg tattcataga gcatattcca gttttctcaa cgaatcttct
    19321 gacatgaaag ctcgcgttcc ctcgaataca accattgcct accaggacta acggtttttc
    19381 agcttcaaga attaatttca ctgctttttc tatgagagac ttgttgggat gtgaacgaag
    19441 gacttctgat ggtttttcga taggccttat tctggattcc tgttttgcca catcctgtgg
    19501 taattcgata tgggttgctc cggccttttc ctcctgagct attttaaacg ctcttcttac
    19561 aatctcagga atattatctg cattacgaat tgactggttc cattttgtta ttggtttgaa
    19621 catagttatc acatccatat tttggtgcga ttccttgtgt agcatatgcg aatcagtctg
    19681 tccagtaatt gccagtactc tggatctgtc catgtttgca ttagctattc cagttgtcag
    19741 attggtggct ccaggaccta gcgtggaaag acatacacca actttccctg tcagcctacc
    19801 gtaaacatct gccatgaagg cagcagattg ttcatgtctt gtcaaaatga atttaatgct
    19861 tgaatctagc aatgatatca taaggtcagc gttttcctcg ccaggtaacc caaaaatgta
    19921 ttcgactcct tctgcttcaa gacatctaag aaagagatca gatgttttca ttggaagcaa
    19981 aggtccacat ccttggtttc ccttatttgc gacctgcgac attacttttt ttctccttgt
    20041 attatgatgt attggaaata ttaactattg ttagtataaa cagtagcagc tggtgttatt
    20101 gatttatgat aatgttaggt taaattacta gttttgcggt ctgtgatgcg gcaatattaa
    20161 ttggttccca cacgggtgaa gctggtggaa catagcatgc atcatagctt gccagatctc
    20221 taatgtctgc tttaagtaag agagctagcg caattaaatc aattctctct tttactccct
    20281 caccaccaac aatttgtgag cccaacactc ttccgctctt tctatcggct acaatcttta
    20341 tccaaattgg cttattgcca ggatagtaac cagctcttgt tatactttct atcacttctt
    20401 caacaggatc aaaaccattt cttatacctt cttcgctagt taatccagtc ttaccgataa
    20461 acagatcaaa taccttggtt atagcacttc cagctattcc tctaaatctt gtgtttcctc
    20521 cagctacatt ttctcctgca actcgtcctt gtttatttgc tgttgtgcct aagggtatgt
    20581 acgtgtcttt atttgtaatg tagtttctag cagtcgcaca atcacctgct gcaaatatat
    20641 ccgggatgtt agttctcatg tactcgtcta ctttgatggc attagcatat cctagttcta
    20701 caccagcatc tctagctatt tcagaattag gtctgactcc agtcccaaga actatgaaat
    20761 cactatcaat atcacgttta gcactggttc ttataccctt aatctgtcca tctcttccaa
    20821 ggatttcttc tacacgttca cctagtataa ttttcacacc gttagcttcc aactctcttt
    20881 caacaatctt ggccatgttt ttatcaagca aactaggaag aatatgatca gccatttcta
    20941 ctactgtcac atccatatct cctcttcctg tgtggcctct cttcttaaat gcctcaacca
    21001 tctcaaggcc aataagacct gcaccagcaa ttatagatga atgaacatct cttgttcttg
    21061 tagaatcatt aatttttact ccatcactat aatttcttat aaaaaataca cctttttgat
    21121 taacaccttt gatattagga actactgccc tggcacctgt tgctactacc agaaaatcat
    21181 aatcaaatat agtttctttg ccactttgta aatctgttgc atgcacttgt tttctaaaac
    21241 tatctatttt atgggcgcgt gtgtttacga ttacatctat atcatatttg cttttgaaaa
    21301 catctggggg cctttctatt aattcttcaa aattatttat tactccttct atgacataag
    21361 gtataccaca cgcagcataa gaaaccacac tctcttcttg aattatcttt acatctgcat
    21421 tgggatcaat tcttttagct tttgatgctg cgctggttcc tgctgcaaca ccaccaataa
    21481 tcactattct tttcttctta ttagtcgaca attctcgggc agacttcaat taagaagctc
    21541 tattatatca acctgtattt tctgcagtgt actgcgaggc agaaaatccg taatccgaca
    21601 caatggccgt tctcccgttc ttccagattc agcatcattt ctaggaggaa atggcaatag
    21661 attaggctcc catcgttggt ctaaattctc ataacgccat tttgactata caactctacg
    21721 gagtactact taagacactt aaagaaaaaa tcccacatgc tcaccaaaaa cacggcatgt
    21781 tagactcaaa cagacagaca tgtactagaa cgttattatg aagatatata ttctgactat
    21841 gcctattacc agtgtcataa ttgagactgg taattcttag ggatatttgg atagcctgtt
    21901 aacttatgga cattttgtgt taacttaggg gatatgatta gttgtagctt gtagcacaat
    21961 agata
//
LOCUS       DAOR01000023            4883 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_124814, whole genome shotgun sequence.
ACCESSION   DAOR01000023 DAOR01000000
VERSION     DAOR01000023.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_124814"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1401 a          998 c         1020 g         1464 t
ORIGIN      
        1 ttgtattctg acttctattt tgatatcact acttttcaaa tcatcagaat ctgacctgtt
       61 tataacctgt aatgagtttg aaaaacgttt atgttgacct tccaaaggat actttccaac
      121 atggattgaa tcttcaatca tctgacatag ttgtgaaatc ttcacagcta tttaatttgc
      181 caattctaaa ctttaatctt tcgttaagct catcgtggat gctgcgtgtt ctctttattt
      241 cttgcctaac attgctgcat ggcttccttt tgaatatggc cattgacaaa agaaaccttg
      301 aagatcatat attgttgata ttctataatg gataagcttt tgttggatga tgcgatatga
      361 agatacaaga gctggctcat gtaaggggtt gattggaaac ctcgatagca gttgttgcca
      421 accgatgcgt tttctaccac atgacgctat ggggctacat catcttacat tgcaatttaa
      481 tttacatgca ctctttacat cgcgtgcttg gaatgtcatt gaagataaat ttagaattta
      541 ggtatgcttt tatgtttcaa attgaatata agccctagtt gatgatctaa aactatgaca
      601 tactatgagg aagttcggga gctggaccgg aaccctatga tggttcttgg ttttgtgtat
      661 taactgataa gaatcattta tgccaacata cttttccgta atgacaatac accaagcact
      721 ccagcaagaa tagcagttgc acctagtaca gataaatcca gtgctagtcc ttcattagaa
      781 ctataaaaga tcgccatctg tagagcatga gtgccataga agatagggac aaaatgagaa
      841 attgtctgaa gccacgttgg aagaaaggca gccacagtta taccaccact aaggaaaaaa
      901 agatctatag acagagggat accgatggct gtaaccctct ggaaccggcg tgaagctgca
      961 cctatagcta ctcccagacc cgcactagct agtgctatta gcagaactat caccagcgta
     1021 gaaagccagt aaatcgtacc tgcaggtcgc agataaccgg aagccgcacc taccacaagc
     1081 acgacagccg ctataagtag tgtggtaatc cagcctgata gtagcttacc agcgataata
     1141 gcagccttag aaatgggtgc tagcaggagt tcctttattg tagaatcttc ccattctctt
     1201 gcagtagcca gtcccgcgtt gacgatacct gccgtagtta atagaagtac cagatttggg
     1261 accagctcga acctaaggag atctatatcc tgacttcgca aatctgtctc tttgacacgg
     1321 atttggatgg ggctattgtt tccattaatg gcgctctgac cagcatagaa cattgtaata
     1381 gcagccggca gagaacgacg taggtcgttc gtaaaatcaa ggtttagatt gttgatatga
     1441 atggtaactg ggtccggttt tttagcattg taagcagcgt caaaattgct agggattgtt
     1501 ataactgctg ccaccttgac ttgcttcaat gcatcctctg ccttttgtgg cgctatagtt
     1561 atcatagcta ccttaaatgc accagaatcg ctaagaatgt taataagttc ttgagcatga
     1621 gggccgctat cttgtactac tagcgcgact gggttgcgtc ctaccgcctg agctccgaaa
     1681 tacaagataa ccataaatgc aattgctgga agaacagtgg cggcaattga tgttggctgc
     1741 cgcagccata cgctaatgtc ctttcgtacg atagctagga tcggaaacag gctgttaatc
     1801 aatgcacaac tcctcttcct ctaccattga taagtgtaag agcacttatg gctactatga
     1861 cggcagcaaa ccctgctaat atgaatccat taattgcagg gcttgtagaa gtagtggtaa
     1921 agttatgaaa ggcatgttgc aagatggcaa tgccgtagta taccggttgt aattgagtta
     1981 tagttgctgt gacctgatcg ccccatatag ctgggccgaa agcaccactt atgaaaaaga
     2041 ctggaataga tagacctatg ctaagtggga tgacagcttg gcgacgacgg actagagtac
     2101 ctataagtga tcccaaggca acgaatgtta tcatgagtaa caagacgaaa tcaaataact
     2161 catcccaatg ttctggccag acaccaataa gaaatacaat cactattatc ataacaacaa
     2221 ccgaaaggga attgagtaga agagcaccaa gaattttacc aatttgaatg gcccacaggc
     2281 tagccggtgc gagcatcagt tcctttattg tccctttctc gtattctctg gcggcgtttg
     2341 atcctgcttg aagtagtcca ccgagcatga cgctgacaac cataaggctg acaactaaat
     2401 attggacata gcctgtgtca tgaaaatagg tatcgacttc atgagctcta attactactt
     2461 gattaggaaa agcactggca taaaatgagg ttattgccaa tggcaccgct ctgcgtatat
     2521 cgtttgtaaa gtctacttga aggttattga gaacaacttg taactgtgtc tgtttgcctt
     2581 gacgtaacaa tgagtcaaag ctaccaggca cagttacgat tgctacaatc tggccttttt
     2641 tcatcaggtt ctgtgcatcg gcatttgtag tttgttgaat gataaaagaa tgagagtgct
     2701 ccattgctga gagaaattgt tgggcgtatg gacctttgtc ttcgagaact atagcggtcg
     2761 gagcttgacc accagtcaga gcaaacaaaa gaaaaagcag aaggaagtta ataggaataa
     2821 taatacttgt tatcataaat gctcgttcag ttaaggagct tcgtatatct tttatcgcca
     2881 ttacccagat aacaaggagg gaacgatatg ccgggtaaat gttcaatatg ttcatctgtt
     2941 actcgcctct aatctcttaa gccactccca gtcaaactca agaatatttc ttccagattt
     3001 ggttctctct gcgagatttt cttgatgttt atctcctggg ttacaagtgt aataagctgt
     3061 cctgttaaag aaagttcatc tccgctcata gttaccttga gtattttgtc cttttgaatt
     3121 acttcgctta caccaggtag gatactaagc tttgcagaaa gctgtggcga aacggatgat
     3181 gctgtttcta tctccatgac ggtatcacca tatcttcgtt tcaagttgat tggggtgtct
     3241 aaggcaacta acttgccatg atcgataata gcgagtctat cacataatgc gttagcctct
     3301 tccaaatagt tcgttgttag gagaacagtc ttgccttcct tcttcttgag atcaaggata
     3361 tagttccaaa tagctctacg gctctgaaca tctactccca atgttggctc atcaaggtac
     3421 agtagcaaag gatcatgaag aagtgctcgc gcaagggcaa gtctacgctt catacccccg
     3481 gaaaaagaac taacacgact atgacggcgc tcatatagct gtaccaggtt gagcatgtcc
     3541 gtgatcttcc tatcacgctc agacctacgc acaccgtaca gcgtcgcatg gatagacaaa
     3601 tttgtccagg catcgagttc ttcatacagg gcagtttcct gcggcactga accgagagca
     3661 gcgtatacga ctctagtatc gtgcataatg ttgtgtccaa gtacttttgc ctcaccagaa
     3721 gtaggttttg acaatccact gatcatgttg atgattgtag tttttccaga tccattgggt
     3781 cctagcagac cgaaaatctc ccccctcctc actgtaagtg aaaatccatc tacagctgta
     3841 aagtctccaa aacgctttgt caacctgttc agttcaattg ctgggctagt attttctgtc
     3901 tgcttcatag aatgttgtgc cctagcgtgt ttgtctgtcg cagtaattca ggatcagcta
     3961 gacctagttg catcataaag ctaaggtcat cacgaattgc tttgtgttct gctctcttac
     4021 cctcttcaat tcgatatatg tggattgcat cgtaggatat gctacgtcca gttggaggaa
     4081 taacaaaaaa gtttcctgta tgtttgccac ttgcatttgc agcaattatc tcttgtacat
     4141 catagtgaag accagtaaat gtacttcgta gagactggac aacgtctata gatccatctg
     4201 gacttctaat ctttgatcta tatgagtatg aacacccttg agatgcaaag ttgacataat
     4261 acgggcttgt aggtttgtta acgttgctca aatctacagt attaatggtt tcgaaatctt
     4321 gtcgaactag ccttatgttt tcttgtatcg agtttgttgt tcctttgtcc attttgtctt
     4381 ctccactctt ctcctctttt actgtttcat tgcagaaagt tcgggaacac tttcattgtt
     4441 agaatgcatc gtcttcgatt gtgtctgtca tgttatttat ggtttagtga gctaccattg
     4501 atataatgaa atctcgtgat aagtatgaca tattttcaga tatcctaaag gaggctggtc
     4561 gtggaaaata agggatcaag aatgaccgaa gtaattaagg aagcatcctt gaatctagta
     4621 agcaaaagag aatgctcgaa attcgtctgc tagaatccaa aagccaagaa aatactttaa
     4681 aaactactgt aaaagagttt ttttttatgc aagatcacga gaatatgatc ctaaagcata
     4741 gtccggactt tttgacatat tctggttttt ttagcttgcc aaaattcttt ttgaagtata
     4801 atttgacgaa atctatctaa aatggggaga gatctagtct atgaagctcc gtgtattata
     4861 actagttgta gtacttacat aga
//
LOCUS       DAOR01000024            5714 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_23636, whole genome shotgun sequence.
ACCESSION   DAOR01000024 DAOR01000000
VERSION     DAOR01000024.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_23636"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1848 a         1024 c         1108 g         1734 t
ORIGIN      
        1 cgtcgatacc taggactata tacccgccgg aggtattagc catggcgcaa aaatcattgt
       61 atagttgatc gctctttctg ttgaaatcgt gtcctttaaa atcgaaagtt tcaccttcga
      121 tggataatat tggcagaaga ccatcaataa cttggatctt ccagtcttct agtttaggag
      181 gaattatagg taaagtcgtc agatagcggc agccatttta tttaatcgct tctttttagg
      241 aataataaag tcattatcga ttgtcggtaa tcactaggat ccaaagatca tctaggaata
      301 tcggcaggac ttcaaaagtg agattttaga aactcaaaag taatcttctg attgaatagg
      361 aatagttaat cttagataat ataactcacc tttgttagtc attaattgag acacttttaa
      421 attttatagt tgatgttacg tagaagtcat ttctagagca ctcatgccac ggatgacatc
      481 atcaaatttc aataataagc ccgttcatta atgcaactaa tgactttcaa aaaataccac
      541 catcctccta atagagaaga tatgagaaaa atgagaaaat cagtcggttg tggacgagtg
      601 agtgtataaa aggttatgtt attccactgt gatgaaaaga tggtgtacat tgcctggaac
      661 ctaaaatcac agaagtgaaa ttcattaaac cttgtaactc atgatttctt gtttcttcct
      721 ggctttattc attccaaaag aagtgatgtt ggctcatcgt ttggatcctg aggaactaac
      781 tcaccacaga atgcttttgt cagtatcgcc tgatctcttc gtaacatcac ccattgcttt
      841 tctagatact tctaaaagaa acttttgtgc ggcattttgt ataagagttt cctccctgtc
      901 actcacaaag aatagtcagt cttttccatt tatttgcttt atctaggaaa gtaaggtggt
      961 tgctgacggc aatcttgctc atacgtatgt gcatcccacc aatttccttc atggattaat
     1021 aattgtaata acctgtcaac actaagataa attaaatcga aagagttcat tcagattaat
     1081 taaaagtaat tctaaatttt ttaaaattac ttttatacta ctcagcacaa atagaatcat
     1141 atctacgcat actcaattcc gaaaaacatc atattcgcca tctagaagag ggttcggaat
     1201 gtataatgtt tattagattc attcattatt ttacattatg ggaacaacac tgaagattaa
     1261 ctttggtcca catggatcag tagaagttag tacagataac ataacagatg ctaagagtac
     1321 gttaaaggaa ctagcagatc agtattttcc aaagcgtagg taaggaaaaa gttcggacaa
     1381 gtgtacccgg gagttatcgc atgaaaactc cgtcaaattt tattaccatg aatttccaaa
     1441 atgagaagta cggggaggtt agcataaggc aatgtctact agattgaact gaaagcctaa
     1501 aacgaaccac tgagaagagc gaactaaaag aaccttcaaa cttcctataa ccggccaagg
     1561 caaagtggac aaggataatg ataatgatat agcaccgttg ttagagaagg agtggtcctt
     1621 cgtgaggttc tgcgagtgac cttaacatgt ttatgattat ttaattcatg gttgacatct
     1681 ggatagagaa atacaggtat aacaaccgag gtttacaatt gtaaaatatt ataggctagg
     1741 attgcaaata atatgtcata tgccatttag ccttactgat tcagacgtag ccatcctaaa
     1801 aggattgcta aaggatggcc gcaagtcctt tcgacagatt tcacgtgaga caggaataag
     1861 cactccgaca gttaaggcaa ggtttgatag gcttgtaaat atcgggttta taaaatcagt
     1921 ttcacccatt cttgattttg aaaaagtaga ttatagtgac aatctagcga ttgataacat
     1981 gttattgcag gctactaaaa ataaaagaca agagtcagaa aatagtatca agaaagggat
     2041 agggattcaa ctaaaatgtg atttttgcag aggttctatt tcaggcaagc cgcaggtgtt
     2101 taaattcgca aactttgaga gatttttttg ttgtaccgaa tgcagatctg cttacaagaa
     2161 aaaatatagc ggtaggatag aagcccttat ggaaggacat atcccataga agtacctgaa
     2221 agtgatggta gtggctccca aactagacat aaggtaaaga taagataaag aatattatta
     2281 aaaaattcaa cattcccaaa tcgccataac agatatcttg tccggctata catgaatata
     2341 catactgggt gattctatat gctatcttat gggctcctta caaatccttc aaatgatata
     2401 ttaagtgaga taagtaaaat atatgaccta aatttcgatt atgctgagtt aggtatagag
     2461 ggaccggagg gcaatccaca gataattaac aagaagaaga atgaaatagc aagcctactt
     2521 cagaagttca agcagaggcc tatcggtcac acagcatatt ggatagattt gtgttcagat
     2581 tatgactacg tcaggcatgc ttggatccta gaagccatga gagaaattag aacggtaaga
     2641 atgataggca tagatctaat aaattttcat gccaatctaa acggtatgtt ttacggagaa
     2701 aagagaaaga tccttttgga taatttggta agaagtctca gagaaatcgt aagatatgct
     2761 gaaaagtcta aggtacatgt gatgttagaa aatgtaccac tttcgaatgg aatacatgat
     2821 gtggttgaat tcaaatatat tattgataat gttgcttcgt tgtttgttca tttagatatt
     2881 cctcatgctt ttacttccgg aggcatgaag tcgatattaa attacataaa tacatttgga
     2941 gataaaataa ttcacataca ctggcacgat aaccacggaa caaaagatga gcatcttcca
     3001 ataggtgaag gcttggtaga tcacggaaag gcagtcagag cattaaagga tatcaattac
     3061 gatagaacaa ttactttaga ggtttttact aatagcaacg atgctaaatc ctctgcggat
     3121 aaactgaaat tcatttggtc taaagaatct tattaatacc aaatgtgtta gattgctctc
     3181 aaaaaaagaa ggcaaggtca aatcaacttg cttctagttc tttgatcctt tcgactaccc
     3241 cttgaccttc tttttctagt tcatccttgt attcctttaa caatgcaata cgttcctctt
     3301 tggtaagata acttcttgcc ctttgaaagc ctgttccgta acaaccataa cccataaaat
     3361 cattcacctc cttgtttttc catccatatt ggacatacga tatatgtatg ttcgatgtat
     3421 ttatatatat cggatgtcca acatattgta ttattatatg cgaagatttg ttggaggtaa
     3481 tccttattgt tgtgacatga gaggtatgtt aggattctta attctttttt tactgtcaaa
     3541 aaaatccatg catgggcagg aacttgcaga ggagattgcg aaacgaaagg gggacaaacc
     3601 tagccccgga accatttatc cagcactaaa aagtcttcga gaaatgggat ttattagtag
     3661 cgaagaaaaa gaaggcaaga caatagtcta tagtttgaca gagaggggga aaaatgcact
     3721 aagaatttcg aaacgaagat tcataagaac ttttcttggg gttttccctt aaaactcgct
     3781 tctcaagtcc cctcgctatt gctttattat gtcttctatt tatcatgtcg ggcaattatt
     3841 aaacgcggga agtcaaggtc aatcctaaac aatgtcaata agcattaacc aatcgtaata
     3901 tgaagaaacg gcctaaacaa atggtgtaga aggtgcaaaa gggatatctc tgctaaaaat
     3961 ttggaggtga tgcattgaca ataccaatat ctttacaaga atggagagcc ctgaaatgga
     4021 tattcaccga cgaggagcct gcgaaaacag acaacccagt gcaatgcacg acttgtcacc
     4081 gtcacacaca ggaatactat agaatttcag gtttgtactt gtgcttacgg tgttttgcaa
     4141 aatgaacacc tgtatgtatt agatcgttag agagaaagtg tcatccgaac ggctgactct
     4201 ctgagaaagc ctcttatact ataaggacaa caatatcata atatcagtta ttctccacca
     4261 aattatataa tagtaatgga ttgttcagct ttttatgcga accttgaatt tttcaacttt
     4321 cgcggcttag aattttgctg cacataccca gtacgcactc tccatttgaa tctaatactt
     4381 gatcaccagg acaaatacat gagttgcccc cttcttgtat acaccgacta cacctacaat
     4441 agctagtcac tgttgtccaa aaatttccta aaacctacaa cgggttggaa tgtctccact
     4501 atctgtgcgc ccatgttgcc gtttaatctg ctaagtaagc cggttgccgt tactgtaact
     4561 agattagtct tcagtgccat aacatacact agatttttcg atcaatccat ccttattgaa
     4621 ttcaaacttt tccattacag accagtatat tttattgtct ggtgtcccct tatattcgag
     4681 tatcactata ttatctttca agaaatactc tactggaatg aagtgtagat ttggaaattc
     4741 ctttaagcca agagaaaaat attcttctaa atccttcttg tttgtgatca ttgcagacgt
     4801 tcgattagga tataccaatt ttactttagg actatggaag agtatcttct cagagtaatg
     4861 ggaaagaata tctttcagac tatggtcatt ccatgactta gtccatctat cgatgtgacc
     4921 tgtatgaaac cgttatagcg ccggaagaag aatagtcatc ggaagagtga ccggtgacgg
     4981 aaagtgagcc ggcaagaaga aattgaccca ctgcaattac ttgcatttgc acgttatcgg
     5041 gctcattatt gcaataggag caatgttagt aataccaagc gctcctggcc acgtggcgaa
     5101 agcaagtaca tgcagtacct cgttcggtac acatagagtt acgttgtcaa gtagttttac
     5161 gacctcagga agctgttcag ccggggtcgc taccgggcag acgacaggag caggaagaat
     5221 aaacggcggc agtaagtcta gttgtacggc tttctctgat agccaggagg ttcatggtta
     5281 tcagctgtta gacagcaacg gtgccgtcag ttgtagttcg cattctccat aattttttct
     5341 ctcgattaat ccaaagctag tgttaatgct agagccctaa attgggattt ttcagcacgc
     5401 ggcggcttgt ctatattaga atactaatta atgtaatggc agctttaagt ctaacgctac
     5461 tcttgtattt ttctggtcgg agtagtttta ttctcttcta aacgcttgcc caagcataat
     5521 atttggactt cagtgacccg gtgactttgc aatttaatca aataaattat tgccttattt
     5581 tggcgcgccg aaaattgatt ccttacgtat atttgtgtgg atagtctaga caattctttt
     5641 tttgagacca attgttttac gataatattc gatttctttg attacacgaa aagcagcagc
     5701 cgaaattggt aaat
//
LOCUS       DAOR01000025            3570 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_18069, whole genome shotgun sequence.
ACCESSION   DAOR01000025 DAOR01000000
VERSION     DAOR01000025.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_18069"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1128 a          668 c          688 g         1086 t
ORIGIN      
        1 gaatgaatta gccatgttgt atactaaata tttatcgtta gatcggtaag tgtgcctctg
       61 agcttccatt gcgatttgag aattcaagaa tcactccggt tactggatta taatcaaatg
      121 ggtaagcatc gtttgcagaa attggtatcc attctattgc attttcgtta catgaaggac
      181 attgaactac aataaaacta catcttaagt tcagatacga tgcgcaccaa aggcatgaat
      241 tacatagtaa gaaatctatt tttttgatcc gtgaattgtt cctatcatca tgtagaatat
      301 tattttttga cagaggatga taaattgtct cagcagaaga cattaaataa taagcactta
      361 cgtactataa aactatcctg tgagttttgt cttaacactg aagtgttaat tatttgacaa
      421 tagtcggaaa aatctcttca aagattagca atcaggtgga gagcatagcc gatgaatatg
      481 tttccccctt gaggtatata ttgatagtca agcacacagt tacgtctcac aatgatttaa
      541 cggctattaa tgcttgataa ttctcctgat aaacagactt tatctttact atctatgctt
      601 ttcatttcac gatgaggttc cagcgcaaag acaacagcaa ttattataca cgaaacatct
      661 aacatattat acactacaac atggatacgg acgtctaaaa atgattcatc tttatggaat
      721 aaagagagag gcgaattatt ttatgggtat ttatcactta agtgaattca aagacaacat
      781 tgaattcctt tgtaagaagg gtttgtcatg caggtggtga ttgttatgca gcggctatat
      841 ctaaaacgtg cattttacga agcgttttaa gtcattacga cgtaaaaatc gtaattcgtg
      901 aaggtttcag cataacatca tcataatatt acaaccatcg aacctgggtt catatatatt
      961 actgtatggt tcaaagagaa ttgaaggaaa tgaattggtg atattataga tgcgatgtac
     1021 aagctgtgat acaacattac attatcatac tgctggcatg acatgccgta actgcgggct
     1081 gagtgttgat catgaattgt tgttacaaga gaaatgtgtt tcatgtgaac accttctaga
     1141 agagtctgaa aagacaacag gcatttgtgt attctgtcaa tacgatatag tgaaaggcag
     1201 taaataataa cttcaaactg gataaacaaa taacttaccg agaagcttgt tttgtgattt
     1261 aagaagaaaa gtagatgatg aaaagttctt tttgttttgc ctcgggctgt ccttaccgcg
     1321 tagaagcaat aaaggacctg tcaaccaact agataacaat gctggcatca ggaattaagg
     1381 cattcgccaa cgatagaatc accgccgact attgctgtca ttcttattca ccttgaataa
     1441 gtgtgccgca gattcgtcga accgctttgc tataaccgct tcggtaaagt aacgcttttg
     1501 gagccagcaa acggcattat ctacttgccg ctttgttttt gccgcatctg acccatcatc
     1561 tttgacatgg ggctttagcc tcttggtcat ctttttcctt tattcgccta gtgttagtag
     1621 gtaaacgtat gtactaatta aggtggtaag taaggaactg gttaataggt gaagagagga
     1681 ttgaaggact aggaaaaata taacatctta cctagtcttt ttcagatcat cggaacttac
     1741 cgaatgatct atgataatca tatggtggga tactcggtgt tttctagtag ggaatacgta
     1801 acaatacatc aaacaagata aattcatgaa cagaacgtga agtcagttgc ctatatgaat
     1861 aacaaaccgt aagaacgatt agagtggtag ctgagtagga aatatcggac atattcgctg
     1921 cgatagattc atctgggttc aaatcaatcc atgcttctaa gtactacgca gaaagagtga
     1981 cgtcctgcaa ggataatctg tacaattaga atcccacgtg cacggattct acatgattat
     2041 atatacttcc agtgagcttc acgtccgtcc aataaatctg ctctcccaag aagatttgaa
     2101 cctgtgcata cggaggccgt caggattgtc ttagttgccc gatccgcaat ccacttcagg
     2161 agagtattat tctttacttc ctacgtgtcc cccagctact tgacacgata tgtaaatccc
     2221 gagccggaca gtcgtcgata gtcagatctc ggatgaagcg taggccgcca atgacacacc
     2281 tgcactttcc attctgagag caagaggatt cggaaaggtg acgattcttc gctcaaatgg
     2341 gttaccgaaa atacctcaat aggtcatgta aacatgtttt agattcgttc gttatctatc
     2401 tgtttcccat tttgtttgtc tggatttccc gcgctataag aatttcgcac tgatgtacat
     2461 catttgccat tctagtgctg tcatcaggta attttacctc gggctatcca aacgacatgc
     2521 tagataagat tttcaagatg tcagagatgg agacaatctt caaacgtgta agaacagatt
     2581 ggcgtgttgg gtatctcaca atcttttcag aggacgatat caacaagtca aaaatggcag
     2641 taaacacgtt tatgcgtgaa atggaattaa ttcacgctgg ctttcagggc aatgtgtatc
     2701 ctgaacatat tatttctcaa tgtataaaag attataacct caatcggatt gacgtagagt
     2761 ttggcaactt ttctcttggc gttgatttag gtagcacagt gacaggtata accctcttac
     2821 agtggaatca ggctgacaga aaagtgtatg ttatcctcag cgatgaatat gtgaatatgg
     2881 aatataacag aatattacgg aaggttaatc aattgataaa tactttttct ccagataaaa
     2941 tctatgtcga tgcttccaac agttttctga tccgtgatct taaacaacaa ttttatgaag
     3001 aaccatttga aataatgaag aagcgaataa cagaagagta taaagatcaa ggcatgaaag
     3061 acagaccata tgagacttgt gaaaaataca tgcgtgtttt accaatcccc tggaataaag
     3121 aaggcgttcc aatgatgcaa catagtaagt atgtcatgga acgcaatcaa ctgaggatta
     3181 atcctatatt cgataaactg attacttcac tcagaacagc atatagtgaa aacgacaagt
     3241 taaacaaaga tctgactttg cacaacgact gttatgatag tttcatctta gcgctgaggc
     3301 gttaccgcaa atgaaagaac agattcctac atgtctacta aactacatta ttctgtatcc
     3361 cgtgccttga acacaggcta tttctttatt ccctttataa tcctgattat tctatcatgt
     3421 tcatcttctc ttcttagttg taattgcctc agcctctcct tatctctttt attagtcggt
     3481 gacattcgta atgccatacc gcaacaagga cagaacacac cgtcgtgaca atagtaaacc
     3541 tcacatcttc tacagtattt cttaccacct
//
LOCUS       DAOR01000026            5626 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_50716, whole genome shotgun sequence.
ACCESSION   DAOR01000026 DAOR01000000
VERSION     DAOR01000026.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_50716"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    594..871
                     /estimated_length=278
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1613 a         1003 c          976 g         1756 t
ORIGIN      
        1 gcttctttgc tgttgtagga ccaatgcctt cgatatcctg aatttctagg tctgttactt
       61 gtaatgatgt tgacgttaaa gtttcaggta ttacttttgt atcatcatag gtcttttttc
      121 tgtttgcagc agtaatatcg ttgttgtaat cattattatg gtcgtttgga ttactatcca
      181 ccaagttgtt ttcttgatta ggttactaac ggttatatta atactgaaaa gtacctggga
      241 gacggggagg ggaggaggaa ttctatagta tcagcgtcaa ttaattaaca taaagaggga
      301 gattacattc ctcacgaagg aagcaaattc tttcaaattc cgagcacaat gtcattatgt
      361 catggcagcg agggcattca atttcttgtt cgatttctag attatcgtta ttcttctctt
      421 ccttcgtttg tatgatgtac tcagggcgtt gatcaagctc tggtacctct gtcagtaatt
      481 tactcctgct gtacattgct gactatgcat gctcaatggt ttctaatata gagtgaaata
      541 ccgatggggg agggaggatc tatatctgtt cgtcctaaag ttcgttttaa ttcnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccatttct gcagtaatca gctactatcc
      901 attgcgatta ttgggctgct gttatttcgg ctgcttgagt aaatcctagc atcgcgttta
      961 tcattactgt aacttgtagt cacgcagtta ctcgatctag gatagttagc tgagtgatga
     1021 gcaccaacaa atgaaagatc cgaaacaacc tgaagctaaa ttatgtggtt actactaacc
     1081 tgctttgttg ttagagcatt ctcggcatcc ttctctcctt gttttattaa ctttttttat
     1141 ggtgctaatc gaaaaatcgg catcctcaaa tagaaaatgc acatcttctg ttcgctcaat
     1201 tcgtgttctc tttttaatta ttgcaccgct cttatctgct aatttatggt actctttcaa
     1261 acaattgaaa caatatgcat tggatcaatt gcggtagtac tatgactgcg attgcggttg
     1321 aagctatcca agaattcctg cagaagaaaa ggaaagataa gtagaatttt atgccgtctt
     1381 cattatctaa attctgtttt tgtgcgtgaa atgtttgtgt ctaaagtagc gaagcagtac
     1441 cttgaaagag taatttgcaa aatggcactt gaatggtggt tgcagattgt agggaacctt
     1501 tctgtgcctc cctcgctttt atatgtgtat tttgattttt taccattttc ttttccaata
     1561 ggaccatcat ctaattttgc atcagaaatc gtgacggtct tcataagaaa aacaaaatcg
     1621 atgttatact gattggtact aaaggcatga ctgcggtaga agaatatttt tttggaagtg
     1681 ttgccaagga agtcattcac catgcacatt gtccaatatt tgcaatccgt tgagacggat
     1741 ataagataat gtgatcgtct tctcaagttt gcgaagttga ttaggacata gtagttatgg
     1801 cattgtaccg acaacttaat tgaaaagtta gttactgaat accaacattc gccacaaaga
     1861 ttaccaatcc atctaataac gcaggatgga tacgttgatc ctaaaggaat actgctgtaa
     1921 caaactataa gaataagtat gtgttcgacg attgttcttc taatgacatc tatgccctac
     1981 tccagaatca tagatcggcc aacaaaaagg attaccagca gtcatagaaa taaacacata
     2041 gaatatccat gcggatgcgg atacttattt gaccatcata ttatattaga gcgcatatgt
     2101 tcagaacatg aaaatgagtt aattgctcat catggctgat attggtgccg ctgtagtaga
     2161 ataattacca ctcttttatc tttgattttt tgaatagata agacaagggt tgatatcaac
     2221 ctcaaagaaa caaaatccgt atatgttaca tccttctatt atttgtatgt tgtagaatga
     2281 tgagccgaag aattttctaa gaatatactt gacctagatg acaagataag atttgctgga
     2341 gtaatggaaa aatcaggaca tctatatgca agatgtacaa aagaaggatt tgaagaatat
     2401 cttaaaggaa agtatcagga aattagtttt tctcagactg cgcatattgt tgaaatgaga
     2461 aagacatttt ccagcgatct aaaatatata gcatatacgt atgataaggt aaaaatattc
     2521 agttttccat taaaggatca aatagtagta ctctctgccg agtgttcagc gaatacagaa
     2581 aatattattg agaagacttt agaatatatc aatacaatgg gagcaaaatt atcgttatat
     2641 ccaccctcta acgttataaa tgaagagaag aaggaaatgt tgagaaatct ccttgaatct
     2701 gagattcctg aggagatgat tgcagagcga ctgatctgct tcctaagtgg atatgagcaa
     2761 attgttcaca ttagctctat agtaatcgag cttatctgga ttgtatttct gtactatatc
     2821 gatactttca ccccttctta ttcttcccat ggctacttcc cggtgaacgt agcctgtcac
     2881 gaatttgcaa ccacgacgct ttgcctctct atgaaactcc aacctaagaa gtcggccccc
     2941 tcctgtccct ccccagtagc ctggcttgac gcaaatagat tcgatatatg cagtatttct
     3001 ttttcctaaa ttttcatcat gcgtaccacg tcgaagtgtg tagttctaca agcgtccgcc
     3061 atttgcgtag cctatgggtt ctccattctt gttaatggtc cttaaagtta cgagaatcgc
     3121 gtggggatcg gttactgact ttttaaattt tttaaaaccc gtgctcattg gagcaggcga
     3181 ttctttgtat atatgcagaa gagattctat catatttgaa ctaatagtat gaattccttc
     3241 aactactgtt attagctcta atagcatatt tctaactgaa cctcttattt caacttcctt
     3301 tgcccaagca tctctgaagg ccattacatt atcagagaag ttcttcttgt actcttgtga
     3361 aacaccatcc aacacacgtt caaacatttc tttctcttcc tttaaggatg taaagtactt
     3421 gacggattct gtgtttataa ctgagggatg ccatcctagt gaatgtgcgt tgcgtcgtgc
     3481 aagttcctta gaacgttcaa aattaccaag gctatagcct gcaatacggt ctgaaatgga
     3541 aagaaaccat cctagatctt cgaaatgttt tcgtatcgta atatcattct caggaatccc
     3601 gccagacgta aataattctt gcattctttt gttcgaacgc tctgctatga gacctttaaa
     3661 gggataggct gtacggtata ttaacgcaat tacaatatct atgcttattt caaaatcatc
     3721 tataaagcgt tttattttat ggtcatttct aatgaacttt tcaacagaat cttcatttgg
     3781 tttatcgaat cgtttcaagt gatcatagtc atgaaaaagg gcagctatga atagagtgat
     3841 gagatctttt tgactaagtc tatattcttg tctcttctgt cccttggctg ccaataaagt
     3901 aaagtaagca acttcaagct catgatcgat attgtgatat ccataataat ccgctccaag
     3961 cccatttttt gaaaattctg acacagcatg tctaattatc ttgcttacta ttttatcgtc
     4021 tagtcccatg cttgtagata acttgattat tttgtccctc agatctgcct gcttagcaat
     4081 cccaatgtta taacgcaacc accagggaac caactttatc cgtttgtaaa actcgtacca
     4141 ccatggtaat ggttcaacca acggattgat ttgattcaaa tcctcaagat tttcattgtt
     4201 aatatcatta tcatcaataa cgtcactatg ttctactttc ttatcacgag gtattgatga
     4261 caaatgccag caccacaaat acacatatgc tgattaataa acattaaaaa ttttatatat
     4321 tatgtcaatc tcgttacact aagatggcac taggcacata aaagctgatt attattcata
     4381 gaatagtcgt cgtctatgta ggaattttat ttcaccatat gactgaatta cctctggcaa
     4441 ttactcaata tctcttcatg aaaccctaag ttacaagcga ttagtacata tgctacggca
     4501 tctctttatt gtatgcttgt cttcctggtc acaccaattt acgcattatc actactgttt
     4561 ataacaggag tatcataaac acctttatcg atcctctgat gacgcatcaa ggcatgggtt
     4621 gatatctttt tgtcaaaata tcgtattaac cagaacacgc caattgacga caagtgtttt
     4681 tcatacgcac ctactgcagg tctagctgtg tgattctatt gattaggaca gaatgatcag
     4741 catctacttc ggtagtcaca tgaagaaggg atggttgagt cctgtttttg agaattttgt
     4801 aaacaaagaa atacttgcag catgattcca gaaaattact gactaaatac gaaagatagt
     4861 tgatatgatg taacaccaac aattattctt taacctttat ctttgcagtt tcattttttg
     4921 atcgtaaatg tcatgtagat tgatgtcgta gcccaaatct gtcatctgat gaatggctgc
     4981 aatatcgtga tactaaattc tagtaggcaa caaaaatatt ttgaaaaata gcgtttttgc
     5041 atctattcca tcgccgacat gtccgcacca ctacatacat ctgtggttct ctgtatcttt
     5101 ttcaaatccg tgtcacggcc cacgggcttc ccgttcgctt atccttcaag tattctctgt
     5161 cttgtcataa cgataaacaa catagatacc caaagactga gcacagctgc ataaagagag
     5221 cgattcaaag caaaccataa aacgtaaaga aatttcacat agttacagac tgctggtact
     5281 tgaacttgac attagaattc caatggtcga cccgataaag tatattgcta taatagcgaa
     5341 caaaactaac gtaagcgaga aaacaaaacg acaagcagtc aatattatga atgaggttac
     5401 gagaagggaa atatctgcag gaaagaatcc tatgggatta tcagcctcag ttgtttactt
     5461 attatgtttg atcaacggag ataatataag ccagagtgtt ttcgcacata ctgcaggagt
     5521 aacagaagtg agttagaaac atatgcagga atttaagagg tcaagatgga ttgttcgatt
     5581 tattttcgcc agcaatctcc aggtaattgc aaataattcg tgttgt
//
LOCUS       DAOR01000027           12211 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_33774, whole genome shotgun sequence.
ACCESSION   DAOR01000027 DAOR01000000
VERSION     DAOR01000027.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_33774"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    8505..8734
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3512 a         2296 c         2072 g         4101 t
ORIGIN      
        1 cctatctcaa atgaatcagt acatcatttt gtaagaggag aagcaagtga atttgttata
       61 accgatctgg caaacatggg tgttgcacaa gctgaattag agatggatgg attgattgcg
      121 catcctgata ttatgagtga gaatgaaata ggagaggaga atgagagact agtagaagct
      181 ggagtaaaag caaaaggaca aagcgtaata gttgaattaa aagatactgt taatggtaga
      241 cgcctagatt tctatgatag tacattcaga tcatatctta ggcagctatt atattatatg
      301 gttatggctg gtatggaaag aggaattatt tctataaggt acaatataaa agaactcagg
      361 tgggttaaaa gtgatttaga aggcgattac ttctttcgac cattcaatgc taaagatgtt
      421 ggcatcgaaa gctgggaagt attgttacca tcacatgata ttgccaggca aatattaaag
      481 aatgagatga ttagacgcaa aaacctattc ttaaaagcat tggaagaaaa tgatgtttct
      541 atattaccta gattgataga tgatgcaaag agaagtaaat gtccacattg cccattttat
      601 gataaatgta tcaaccatga ttctgaaact aatgaggcta aagaaatggc caaagagata
      661 gatttactag atataagagg agtggttgat tcagagaact ccgctactca ttaactgcct
      721 gatcctcctg tagatagttt gttcatctgg tatcttgtat gaaagtcatc tcatacgcac
      781 tcgcgagcgg aaaggttgac tctaggcagt cagctctacg cttgtaggct ttactggtga
      841 atctagtagc aaggctccgg taagaaatac aaacaacttt gttcagcgta ctcggtgtat
      901 ctgaagctct tacaaggcgg aacggttaag cttatggaaa aggctagatc tatcatgata
      961 cactatgcat aaggcgaaaa atgtcacaca tacaaactag acgtaatagg aaccatagct
     1021 ctggaaaact atacctgcat attaatcagg tatttttatt ttgttgaaat ataactaaat
     1081 aggaagatca gtaattaaat tgaaaactag ttataaccta ctacttcaat tctagattgg
     1141 ttaccatctt tagttactct gtatatttta tccacaaaac tctctaattc acgctcatga
     1201 gagaccatta ttatctgtgc agactttaat tcgtctagaa cttgtcttag cctaattaac
     1261 tgttcttttc caaagccatc agtaggttca tctaaaatta gcaaagcatt atccatacct
     1321 gcaaccttct taagcatagt gtttagtgca agtctgtatg ctaaagcaac tgatgtcttt
     1381 tctcctccac ttagactatt gacgcttaga gaatagccac tctgagtgat aattggagta
     1441 aagttcgagt ctgcttctac actaatatcg cctgtgtcca ttaaaatagc gaaccatctc
     1501 tgaaataact ggttaaattc ctcatttata ctctgaagaa catgtctttc tatgtcttga
     1561 accgctggaa taaagtgttg ctctaaccaa ttattcgttt gctgaaagat ctgcatattc
     1621 tgatgacgag cctttttatc tttaacttcc ttttcgagtt catgcaagtt tgattctaaa
     1681 attttgagct ctgaacgcct gcctgaaatc ctctgcctta tttcggacat ttccttttct
     1741 aaacttccga tggtgtcttc taacacttct aaatctcttt ttgcatcttt attcttgttg
     1801 aatagcgctt cgttctcatg taactttcgt tcgtcagaat taatttcaat ctttaaagca
     1861 tcaattgata cattaacatc ttgaattgcc ttttcaattt ttgacttcct ttttatttcc
     1921 tgtaaatttt caacatatct aaattcaatt ctgtcaaatt cgggttttga aatcgtttgt
     1981 tctgtttctg catagagttc tttggctttg gtcaatatcg ccatttcttt tagaatttgt
     2041 gataacttcc ttctctcttc aagagaatag ttttgtttac taagattttg tatgttctcg
     2101 tttatcctta attctcttat ttgaagagtt tttttcgacc tttgaataga atccttcttt
     2161 tcagataact tacctagctc gcgctgaatt gtctttaaat attcttcttt actttcgagc
     2221 ttgtctataa ggattttatt ttctcttttt ttatcctcat aagttgttat cgtactgttc
     2281 aaatatccta tatgctctcc tacccctagt ttttcttgat caaattcggc ttttactttc
     2341 gagatatgac ttaaactcat tttctgcctg catgttggac atggagcgcc ctctccgata
     2401 ctgattatct tctcaatttc cttttgtttt ttggatattt gtccttccat tgttaatcct
     2461 tcagccctat tcttggatat tagagactcg atattggctg atttctctct taatgttggg
     2521 agctccgaaa ttgatttttt taggcttaat tctttctctt ctaatacgct caattctttt
     2581 atggattttt cctctctctc aagtttttct aattcagcct tcttctcttc cgttagttta
     2641 ttggtttctg attctaagtt tcttctttca ttgtctataa attgttctaa ctttgtcttt
     2701 tctttctctg cgatatcatg tagttttact gttggttcta attcctttaa tttatctttc
     2761 aaagaaatat aacgctcata agctggtttt agcttctcaa cgaggtgctc tttggtgatg
     2821 atggaatcca gctctccttt tagtagttca aatctttctt gttcttgctt caaggtgttt
     2881 tgattccttt caatagtgtt attcagaagt ggtatggata cctggagctg taaaacgaga
     2941 acttgtttcg gtttaagact gtctatgttt tcctttagct gctttgtttt tttgtctaac
     3001 tctgatattt tcccctgatc tttttctacg tcagttgtca aaaaaccgac ttttttgttg
     3061 agctcataca atgaagtttc cttttcttga agatctttaa ttatcccaaa atttacacgc
     3121 atttcaccag taccccaatc cagaaatgtc tcagcatttt tcttgactgt actgtattcc
     3181 tcaatattga aggctcttct cagaatccct agtctttttt ccggcacttc tgaaagaatt
     3241 tgcttcatca tttcttgcgg tgtaaaaata gcataacggt aaattacaga tgtcgttctg
     3301 gtctgtgctt tttcattaat attaataatc ttcaaaatct tcgatttcat ctcgcttacg
     3361 gaataagtcg tttctacatc ttcctcgaca atatatcctt cagcttgcgt taccttctta
     3421 tcgcgtctaa ttatgcttcg aaagatctga taattgttgt tattaacttg aaactcaaga
     3481 agaacagatc ctttcttttc tcctgatctc aggagattgt tgccatctac atcacctagt
     3541 ccaaaaagag caaattctat cgcagagaga atagttgatt ttccacatcc gatgtcgccc
     3601 tgaaagagga ttgtgcctgc aggtatgact atttctgtat tttctttata acttctgata
     3661 ttgtgtaaac taattttttt aattagcata ttattttatt tctttattta tttctgataa
     3721 tcccatgaca gtctttgctt catgcatgag tcttttttca aagtcatcta aagtttcgtt
     3781 ttcaattttt tcctttttta gagcaagtaa taaattgttt gccgtaaatt cgccgtgttc
     3841 agatataagt ttggatttta taaaattctt tactttctca tctgtcatag cagggtcaat
     3901 cttaattgat gctattcttt ctttgatcac tttcttttct atctcctcta tgcttgtacc
     3961 aaagactttc acctgggtag tctcaggaga gacaaggtta tttctgttaa tgaatgatac
     4021 taatgcacgc tttgctgaaa gtgtttcttc aaatcttgaa aagttgatat ccgatcgctt
     4081 tcctgacacc atagttcctt tcactttaat caaaactatt ttatctgtta ggtcctccat
     4141 ttgttctaca atcaagctta ttttatcctc tatttgcttg acagtatttt gatttacatt
     4201 aatagcgttg aatataatat caactatctt tgtttctata aagtcggcct ttattgtttc
     4261 gttgtcatta tcataactta taatatagaa tcccctcctt tcaccttgtg cagtatcttc
     4321 taaatctgta aatgtggacc caaataatgg tccaggatag acaatcatac tttttccatc
     4381 atcaggttta tgttcaatcc ttctatgtaa atgacctccg ccataatata tgaatccttt
     4441 tggtagtaac gataatggaa tagactctcc atgagcaagg tcaacaggag taatttcact
     4501 tattgatgca tgaaacagaa atatcttaag gtcttcttca gactctagcg cttccatatc
     4561 tagtagatca taatattcac tatcgaggcc agtctttctt cctgataggc ctgtgatttt
     4621 aaaggcagtc ttcttatctt ggataaattt cagtttgatt ttgtctttta tttgctcaaa
     4681 ttcaataggc tttatgaata gtcctgcgct atgaagtata tctattatcg atactgtact
     4741 tgcagtgaaa tcatgtgaac cgtaaatcat ataaatctca attccatgtc tcctggcttg
     4801 tatcatgata tctactgcac gcttaacatg atccagctcg gggacattaa catcaaagaa
     4861 atctcctgtg atggcaataa agtctacctt ttcttcaata caaatggata tggctttctg
     4921 gaaggctttg agattaattt ctttgagctt tgggttcctc caagcaccca aatgacaatc
     4981 tgttatatgt gcaaacttgt acaaattatg ttactactgc gatcttctta gttggacttg
     5041 ctttcatagc tttcacaata tcttcaaaaa ggggtatctt tatagggaca gcaaattttg
     5101 tgaaattaga actaattata gcttcaccct tgtccagact tgctatgttc ctgtcatctg
     5161 ttgataagtc ctgtgatgca ctctgtatta ttgagcttcg ctcagtcgca agttcatttc
     5221 ccaggattat ttttgtgttc atgttcgcta gtatcgtttt tggaataacg cttgtaagct
     5281 gagttattgc aataagacca attttgaatt ttctaccctc ccttgcaata tcgctgtaaa
     5341 tattagaacc agaagatcta aggacctcgt cacccaaaac cctcggagct tcttctatta
     5401 caattgaaat tacaggcttc gttcccaaaa tgccctcttc ttttgcgctc ttgtggcgat
     5461 ccagaatttc gtgtgctaca acgcttccta tcagaagctc agtctgatct tccaacatcg
     5521 acgtatcaat caccactttt ttgccctcct caagtgtatc tatgatttga gttaccgttg
     5581 attctccgac tgtatcgcta aaagctctcg tacgacattt cagttggttg tcagtatgat
     5641 cgaaatacac tccaagaagc ctgttgaatt ttcttcttaa tacgtttaga gttcccttgg
     5701 ctattctgat ctctggctcg ccaccttcga gatcactgtt gataggagta tcctttataa
     5761 tctctgatat ccagttttca cgatatacct gataagctag atacattgca tcccactgag
     5821 cttctgtgaa atcaacaatt ccttttaagt ggtcaggtgt tatggatctc aggttaatta
     5881 caagtcctac tgctccgtga atggcagaag ctttgggaga ataatatttg agatattttg
     5941 ctgcctgggg gtgatttcgc aaccctatgg cagcggctgt cccaaaatac tcgtcatgcg
     6001 cgtccaaaac aagaattccg cagaagtcct tatctaaaac gtcccacagc atgaccttta
     6061 tcaaattact ctttcccctg cccgtggtag caggaatgag aatatgatgt gagagaacat
     6121 ctggaccttc cagataaacg tctgcattta gggtcttcga accagttctg accttgccaa
     6181 aatataatgg attattgggt ttgattagga aattcaaatc ctcattcttg atgtgtctaa
     6241 cggatcccat aaaattcggc agaatttttg gattgtgagg actaccattg agatataaaa
     6301 tagatctcgc caaagccagc acataatttc tcaaatgagg ctccatgaag tctaggccta
     6361 caccgctgcc ttcaagtctc attcccgaaa tattttcaag tgaagattgt gaaatctgac
     6421 taccatataa gagatcaaaa acctgtaaaa aaagcttgcc ttcatttctc tcctccacaa
     6481 gtagatcacc taattcaatt gctccatcac ttttctgtct aataattaat ttttccactc
     6541 ctccagcaac gatttccccg caacaatcat cattcatcta ccatagcctc cgaccaagag
     6601 attcagaata tgatgtgcat ctgtcgatcg catatgatgt gcaaatttct tccacaaacc
     6661 taacttggac atttctgaaa gaagcattac tctataggtc tcaatctcgt ttgctctaac
     6721 tcttgcattg ttgtctgcat ctataagccc atacggatat cctgggaagc ctatatcaac
     6781 cgaattctta gccaagcagg ttataacttc attgaggttc aaatcatcca aatccttcat
     6841 ttgtctatta cttatttcat atcgaaatat atgatcggct tttttatgta gcttcacaat
     6901 ggtcattata actgagtgat cctttgacag accttctgct agaggtgagt aataccattc
     6961 ctcgaagttt attccccaat cctctacaag tttctgtaga gagccaagca atgacaagcc
     7021 agttgtcgta atcatattac tcgtctttgc gagaccagaa aagattacgc ctttatttcg
     7081 tgcccgttca tggagaacgt caaggtagtg tccttcgtta ggaaaggatg tttgaagggt
     7141 tccatccgat atcagtacat cgcctttatc taggattcta tctataataa agcgcgaata
     7201 attccattca gcaaatcttc ttgctacaga agcaacctct ttaatatctg ttcttgtggt
     7261 tcctcgtatc agacgacgat ctgtggaatc aaatgataag tgtgattcaa atggaagata
     7321 atcgctaaaa tcattctttg tagcaaagag taaagtcttg tattgaatac tattatcatt
     7381 aaggtttaaa agagtaactg agaaaaaatc tattgtttga ggtaattcat tttgatttat
     7441 cctttgtgtt tcgttaaata cgttaaagta tattctattt aactgaactg aaaaattcgg
     7501 tgcccctatt agctcttggt tgccaccatc tacaaatgcg atttttcttg agctgccgtt
     7561 tccgttgatt ggataaaagt tctctcttgc aaaaggatag atcctgtatg catcttgttg
     7621 aaagtaaggg gcacccagat catattcgtt gacgttttgg tgcaatccat ttattagcct
     7681 attgattgtt tcgctcagca tcacgctact ccttctacga tgcaaggaac ataataaatg
     7741 atagtgtggg taaaatgcaa ttaggaaaaa attccatcgc gaatattcaa ggaaactaca
     7801 gatgttatta ttttgtatct tataggaaga aaactaggtt ttttagttta tagagtaagg
     7861 attaatcctt aaccaatctg tgtcaagaag aaataataga gtaggtttcc tctttattca
     7921 cacactctgt gatcttgata atttgttaat tagttgattg ttcccgcata aaacaatgat
     7981 aatacaaaat gcatattaag caccttcccc tctacattat tggttaaact agactgaaag
     8041 caagactacc agtaacgaca aaaactagat tactaaataa ccgactccgc cagcgatctc
     8101 ggtggcgtag tcgagtcgct gaagaagacc tttgtcgttg aagtagaagt tctgttctgc
     8161 acaatgagtc gggatggtcg agatcgttct tgcaagtatt ttccaacgag atcaaacctc
     8221 cgtatcatcc ttgctttgtt gagtcggaat aagaattcaa ttaaatcaag aaatgcatta
     8281 tagtataata ctcttcaggg tgtcgactaa tgtaaagcgt tctgcaaaga ttgggctttc
     8341 ctttgccatg tttgtaaatg tttcagtgtt ttccagtctg atcctatctc ccattatcct
     8401 tgaataaatc tcttgttctg tttgcatacg catatttgat cttatcgtag ataattgtag
     8461 gaatgatatg ctaagcagac ctccaagtac catagaagaa gaaannnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccctt cttttagtag tctcacagtg
     8761 attgaaatat catattcctc aatcagagtt atcttcacgc attgaggata cgcctgagag
     8821 attcacgagt cagagtaata taaccaaatc tatctgcata tttgtcccat tcaacatatt
     8881 tttcatccac tctggtttaa tcctcggata aaagaaagat cttttatatg atatcgcata
     8941 acttttacca taaaatgtta attaggttag aacaatcctt tgttgaatcg gcagaaaaac
     9001 taaacgatag gttttctgag caagataggg aagagctatc gcaagcaatt ggagcaaatt
     9061 atgaaaagat aaggtcaatc atgcttgagc aagagaaatc caaagataaa tctaaaccaa
     9121 cactattaga attaggaaat attggctcta gattttttgg atttaatatt tactataacg
     9181 acgatgtgga tgatgtacca attagtatta gagaactaat tcctaccatc tcacctgttc
     9241 gcttagactt aatggtaaca acaaaaaaaa caaaactagc tgatccaaat agtacttatt
     9301 tgaaggcggc tcgagtttat gatgatggtt caataaaggc aaaatattat gctgaagcaa
     9361 taagagagga tgaaggaatt agaccaccat gactaattcc gaccggctct atattactgt
     9421 tcgaatgccg actggattgg ataaacacct aaataaaaag gtctgccatt gactatatgg
     9481 ctaaagagca acaatatcag aaggtagcaa acaaaaaaga tttgaaagaa ggtggtcttc
     9541 ccaagattga acctaaatag aatactaata tagttaatat atactatgta ccttcataat
     9601 gtcagctagc acagacgtaa tttctttctt atatgttgat gtactcgagt attcagaaga
     9661 aaaaaccttt gtagctgaat gtttggaaat accggttata gtagaagcag aaacattaga
     9721 caaagtagaa tctaaaatga aactcgcaat agagggtttt ttcgaaacat tcactgaaga
     9781 acgcaccgct gttcttaata agcgaaggat acctgttccc aaccgacccg aagtcatacc
     9841 aagtgggaac agaactgttt tgaaaatggt gatgccatac ggttgaaaac tcacattatt
     9901 atgattgaga atacaggcta gactacttaa tggcccataa acacaattgg cattaattcg
     9961 tcgtagtttt ggaacagata ggatatagac agattatcga ttatgatatt gacattttat
    10021 actttattaa ccagagcata ggtaaagaaa ttgggtttga aaaatcgaat cacataagta
    10081 tgccatacgc tcttacactc ttacactatt acgcagactt ggaataccat atgaccggtt
    10141 tgtgcgaatt tataccgaat atagtgaagg aaaagttaat aaaactactc aaaatttatt
    10201 cgatgatagc taatggcttt tggattcaaa tagctataaa gaacacggga tagatatgct
    10261 tctaagatgg cactgttttt gagtagacat ttaaatgcga gaaagacaga caaagatcaa
    10321 attaatcaac agaacatccg aaacttatac acggagcatc caaagatgta tgccgccatt
    10381 tctaacttac accaaggcgg cagtaattat atggaaatca tttgcagttt tttgtaggtg
    10441 atacgtgaaa caatatataa gaataacatg aaatacatat caagatgagc aaagaaaccg
    10501 aaagagaaga gattaaggat gaaactcatg agttggaaga cgatgaaaaa gacgaatcag
    10561 aagatgtgaa tgaagaagaa tgaaaagttt caatgacaat gtctcacttc tgttaggccg
    10621 aatgagaaag taaagatgct aatggcagta gccagggcca tcagttttgc aagaactttt
    10681 ttcaacagga tagaagacgg aaaaggaatt gaactaataa tagttacgtc tatattctaa
    10741 ttatcccagc agctgcccta gcaactactt cagataatgc aggatgaata tgaacagttt
    10801 ttgtaatatt gtctatggta ccgccgttat catcggccct catggccact aaaacttcat
    10861 gaataagaat tgacgcctca cttccaataa tatggcagcc aagaattttt ccgttcgttt
    10921 tatctgcaag aaactttaca aaaccatctc gatcttcaag cgcatgtccc attgctgtat
    10981 ttatgtagga atagacagac ttttgatatt ctaaaccttc tttctttagt tcgtgctctg
    11041 tgaaacctac tcctgcgacc tgaggagaac tgaagatcgc gtgtggcatt gctgcataat
    11101 tgacgggaac ttttttatcc gggtgtagaa tattgttggc tacatatcgg gcttcgtggt
    11161 tggcattgtg tttgaataag taccgaccta ttatatcacc gagtgcaaat attccattga
    11221 catctgtttc tagatatcta tctgttttaa tgtaaccttt ttcaccaacc ctaacaccag
    11281 ttttttcaag gtctagagtg tccgaatttg gaattcttcc tgcagcaatt agcagctgat
    11341 ctgatactag ttctaaagtt tttccagacg tgtctttttt tgctattacg tggaatttac
    11401 tactactatt aggctcattt tctctagata cgtattctgt ttcataatct acgtatacat
    11461 tatatttttt tgaaaatatc tctgtaaatt tctgtgaaac ctcctcgtct tcatttggga
    11521 tgaggacgtt tttccgttgt atgatgttta tctttgttcc caaagctcca aaaaagtgag
    11581 ccaattcaca ggctatgtaa ccgccgccga taattgtaag ggtatgaggc tgttttttaa
    11641 gacgtaatgc ttcctcgcta gtcaaatagc cagtaccttc aagaccttta atgtttggga
    11701 ttcttggccg agcacccgag gcaatcaata ttttttctgc tgttatgata tcttgactat
    11761 catctcccac tattgtaatc gttttcggtc caatgaattt gcactctttt ggaaacagct
    11821 taggattatc gacaccttca aatgcgtttc tgatcccgtc tgaatcagaa tcagttatgc
    11881 tgtttactct ctgaactatt ttttcaaatt ctatggcata tccatcaacc tttattccaa
    11941 atagatcagc ccgcttaatt gtttctgcga tgttagcact atgaattagt agcttagatg
    12001 gtatgcagcc attgttaagg caagttcccc ccattctgtc tttttcaaca actgcaactc
    12061 taagaccatg ttgataagca gcattggcta cctctaaccc agagccagct cctatgacaa
    12121 ttaaatcgaa cttttgcaat ttgattgaat aaaagttgtg acatggatat actttttgat
    12181 tacaaagtat cagtttcaaa tgattacacc g
//
LOCUS       DAOR01000028            2851 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_22490, whole genome shotgun sequence.
ACCESSION   DAOR01000028 DAOR01000000
VERSION     DAOR01000028.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_22490"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          934 a          540 c          578 g          799 t
ORIGIN      
        1 aagatgtagt agcagtcagc ttgacgattg tgctaatgct tactaccaat acgcctatta
       61 caagtgctag acatgccatc gaatatattc tatttttttt gcgttaaggt aactcttcat
      121 tgttaacata taacgatttt ttcaaattga cgtcgcacag gtttaatttc cttcctattt
      181 tgtaagccgg aggtcttccc gatgcaaaca aaacatccat ccagcgtgag aaaaataatg
      241 taaaagagca gagacaacga atcatcttag cgttgtgctc ttcctattcg tcacctctct
      301 agaaatataa tggcggttgt accaatacag agctgctgcc atgatgagaa aatcggctat
      361 aaagaataga tcccatatcc atgtgctttc cacactgtta ttcataacat aatttatgaa
      421 tccaaaatcc gctaccgcat ttactaacaa agacaaagag agcaatgacc atggaattga
      481 atgttcataa tcacttctta gtgtaacaag tattacaact gctgggacga ttaatatggc
      541 atcagcaata gggtacgaaa tatcaacaac aaaaactgaa taatcatgat gaatgaattc
      601 tgacaaaatt aagggtatgt gtaccgctag gataatagag acaaatattg ttgatgcaac
      661 tgaggctgct acaataacga tagcattaat cttatttcgg atgaatttga aggttataaa
      721 taaatgaaga gcaaagaaga cgtagccggc cagccagaag acgtccgcaa tggatacagg
      781 aggcggtact tctcttatcg acaagataaa ataatagtat gatatagtaa ggtcggcagc
      841 gaaccacaat aatataccca gtgtaagaca aaaaaatgat ttaccgtgtg ctccatggaa
      901 cttgagtctg catataacaa gtatgcctag actcaaagca atagctgcag tgacatctaa
      961 aagtataatg acaattggct ctcggctatg cgggtttgat aggattatta acgagtctgc
     1021 gatgattatc gagccaatca acagaaatgc aagattcctc agattgattt tgaatcgtag
     1081 atcgtttttt tctatggtca gataaacgct catgattttt atcttataag gatttctgta
     1141 gtctagtatt cgcgtgaatt tctttggaaa aatcataagt ctattttttt caagattgaa
     1201 gcctctggag atggttgctg tccccctact atatggaata ataatgtttt tctttgaggt
     1261 gttgtactat aaccttgatg tacaaggacc ctgtttgtaa catgatggtt gatgagaaga
     1321 agacggaata tatctcccag gcagaaggaa ggaacgttta cttatgctcg gctgcttgca
     1381 agagtgagtt tgagaaaaat tccaccaaat acggctatta atatccctaa atacgtattc
     1441 attcattcaa ggagagaaag ggcgacaact agcgccctac gctgcggtga gatcttacga
     1501 tggtcacaat tttgcgtgct cattcggact atcttttata gcatcctaat tacgttaatc
     1561 ttcacactga gtaaaccgat tggtggcatt ttatttggtg ttgccttttg gactatggca
     1621 aaagacgtgg ccgcaaataa ggtagtaaga gagtacttga ttattgcagg atttggcctc
     1681 ataatgttat tcatttccga ccaggccatt acgcaaattg ccgttgctta ccctccattc
     1741 ggactagtac gatatcattc ctaggattat catcgtacat gatgttagtt gggatctatt
     1801 cttcagcaat ctctgtagcg gaggactcaa acttacgtaa atcaattcga gattttgcct
     1861 taagtcaatc aagattattg gatagcatcg gatcggccca aatggagcaa gaaatagaaa
     1921 aaagggttct agttttcacg aaacaaattc aagataggat gtttggagag ccttctctca
     1981 cagaagatga aacgaagcag tatctggagc aggtgataag agaagaatag atgattattt
     2041 atttgaaaac agagctttca tttttgtcat tacgtaagtt aattcagaca ttcttagatt
     2101 ctcctcgcgc cagaattcac tgccattcag tcttgctgta aacgaaaaat cgcagacata
     2161 taatttgccg atttcagctg ctgtgtaccg tgtaattgat gataatagaa tcggtgggct
     2221 aaaaaaggaa atacgcagac tcgccaccga aatatatgca ctcaataaaa tgacttcgcc
     2281 gagaaacaaa gccataacag cgctgctaaa gctgcaagcc ctaggaatca cagacgatga
     2341 aatagtaatc tgtatgaatt tctgaatatg ggccccccgc tttgaatctg gggtatcaat
     2401 ccgaagataa aacggacacg cagcaatctg gtcctaattt tgggtaatat agcggttgaa
     2461 attagtcgat aaagatttat ctaatatata ccggacaagt aaataatgca aaagcaaaaa
     2521 acggttctat tatttgcaat aacggtaacg gcggtagtaa cgatatcgat cttatcgatt
     2581 ccaacagcaa tggcgacaat cttacgaata aacaccccaa accaaggcca aaaagtacca
     2641 gcagggtcct ttctcacggt aacagggaca tcaatgccat caaacgcaac ccatacaaaa
     2701 tgtagtgttc aattacaaac gaatcagcac ggatacaaac ccgttagtcc tctcggtcct
     2761 gttggtactt atacaacatg gaagggcgca acaagcgagc cagtcaaagt aggagtcaat
     2821 caagtagaag gtcagctgtt atgttttgct g
//
LOCUS       DAOR01000029            3469 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_92739, whole genome shotgun sequence.
ACCESSION   DAOR01000029 DAOR01000000
VERSION     DAOR01000029.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_92739"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1101 a          667 c          641 g         1060 t
ORIGIN      
        1 taccacattt ctatagaagg gggtagtgga aattgctcta cagatttgat tcaatccggc
       61 caacgaattt taataaagtg aagtcaatta aacaaaatcc aagacctttt taacgcctca
      121 ggaagcagct ttcgtggggg ataagatcgc cggtgaagaa acagaaacta caatcctaat
      181 agatttgctc acaaggacat ttggtattaa ttgggtagaa cctgaaaata tcaaaaaaaa
      241 tcaatgatgg caatgccata aagtctcaaa cggaaagaat catgttttga ttagatcatc
      301 ggatgaccta atattaaatt gcaagaaagt ggacaaattt agtttatatt ctgcatcttg
      361 aaaattatat atagataggt tagctgagag taagaatttg ttgccgaatc tattgaaaga
      421 gaggtaagac tgcttgccgc gcacttaata tgtgccttga cattgacatt ccagccagaa
      481 tctgacttaa aagtcttatc tcaagataaa acattcatgc aatcgttaca atataaaaaa
      541 aaattttgat caaaggtatc agaaactcat ggggtcctag taattataca agggattctc
      601 catctagagc cctttaggct taatttatgg tattactaat gttgatccca tttgtcatta
      661 tatttaaagg agatatttag tttttagttc taatgaaaat ggaaagttat attgattgcg
      721 ttatggattc attttcggat ctatatatca gatgtggatt catctgtccc atacgacttc
      781 ttgggctaac gcagaaatgg caatcataga ttcttctagc aacgaatgta ccaagctaat
      841 ggcaatacca ctcattacta tgattcaagt ggttgcacat gcaagaaaat ttacaataca
      901 ttccagaatg acaacttcaa tatttgtttt cattgaatat cttgaatttt cgaataagaa
      961 gaagctccaa gcgacgcgat acattcatcg gtattcatga ctaatacacc tatgtttttt
     1021 aactctagta atgctctatc gtagagaaca gatgtagcat ctgtagccag aacaattctg
     1081 taatctcgct cacttgcttc gtaaatggtt gttctaggac agttaggaaa gttacatcca
     1141 catacaacta ccgtattaac atctagattc tgcaaatgca tttccaattt tgtattataa
     1201 aatgctcccc atctaggctt atacattatc cattccatag atccaatttg ctgaagatta
     1261 ccagaaagca atagttttga attcagtcta atatgaggcg agggtttaag ttcatccatc
     1321 aattcagctc catcacttcc aggaataacc aattgctttc cattttcgat tactttcctc
     1381 cgacacaaat caacactaga tccgtctttg ctgtaaagtc ttataacatg aactatagga
     1441 agacctacct ctctatagcc ctgaactaaa cgcttgatat attgcacaga cgttaacgtg
     1501 cctggtattt cagctacagc acctataaga gtaaaatcac gttggacatc aatgataagc
     1561 agtgcagaat gtttcctgtc aggagtgatg tattcatcgc ccatgatgtt ttttagcagt
     1621 tgtacataat atgaagatat aatatagttg actaaactaa cttactatga tatttgaagt
     1681 ttgtaattaa caacgacttc aatagatatt atcgctcagt atatttgatt tcctatcaaa
     1741 tcctatctta ttagccccaa cctaagacaa gttagttgac caaccacggg aatggtctga
     1801 gacgaccgtc agtgaggcca ttatcaccat tatgaccatc accctatctg aaaccgaaaa
     1861 atgctttaac atgagaacat atcaaacaga cttggacaag ataatatcat aaagttctcg
     1921 agtcttctcc ctcaatatat cgaaaccaaa attatcttta aacaaaagag aagagttatc
     1981 attacgacaa taactactaa tcatgacatt gctatttggt ggaaatatta tcaaatattg
     2041 tatgcaattg tccagacata cgcattatag cttcactatt tttaatagaa atatttttta
     2101 ttgaaatgaa ctcttcgtaa tttataaatg agtttaatga ttcggccgat agaacagaat
     2161 gatgtagaga tctgcggcaa gattggatac gaagcgcaca aaaccatttc ttttgctcat
     2221 ggctatcctt ccgagcagcc ttctgaagaa tttggcatac ggcttatcag aaggcttctt
     2281 ggtaatccaa attcatgggg cgttttagca gagcgacaag gcaagacatt aggaagcata
     2341 ttccttcata aatttccacc ttcgcctgtg gcagtgatag gcccacttac agtccatcct
     2401 tctgcaaagg gcggcgtagg taggatgttg atggatgctg ctcttaccca ggctcgtaaa
     2461 caaaaccatc atcaggttag attagtgcaa tcaccaagtc acattcgttc cttcgtattg
     2521 tatacaaagt ctggattcac acttcgtgag cctctatttt tgatgcaagg gcaaccacta
     2581 aaaggtagga ataatacgag cagcgctaac gttcgcctag ttcgtgatga caacgatatt
     2641 tctgtgtgca acgagttttg caagtcggta tatggatttt caagggaaat ggaattgcgt
     2701 caggcgaaag accaaggcgt cgccgctatg atagaaagag acggtgtcat tacaggctac
     2761 gcagcaggta taggtatatt tggtcacgca gttgcaaagt caaacgagga cctgaaggcg
     2821 ctgattgcta atgcgtctgc aattcttggc cctggtttct ttgcacctgc tcggaaccac
     2881 gaagtaatta attggctgct tgaaaatggc tttcaaattg gttggcctgc aaaccttatg
     2941 actattggtc cctaccaaga accactgacg ccttttcttc catctttggc atactaaatc
     3001 atacatacat aaattatggt catccaaatc tgtgctattt ttgagaggga agttgaacag
     3061 actacgcgag gaacataatc agacagcagt tgcaggacaa gatatttttc aacctaagtt
     3121 attttattgt tgacgggtta ttgcaaatac caaatttgca atcacaaaat ttaaagactg
     3181 gataatagaa cagatcacat aattcgtaaa cttggtttcg aattagtttc gaaggagttc
     3241 aatagcgagt gggtttgaga agcgagtttt aagggaaaac cccaagaaaa gttcttatga
     3301 accttcgttt cgcaattctt agtgcatttt tacccctctc tgtcaaacta tagactattg
     3361 ttttgccttc tttttcctcg ctactaataa atcccatttc tcgaagactt tttagtgcag
     3421 gataaatggt tccgggacta ggtttttccc cctttcgttt cgcaatctc
//
LOCUS       DAOR01000030            7525 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_64249, whole genome shotgun sequence.
ACCESSION   DAOR01000030 DAOR01000000
VERSION     DAOR01000030.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_64249"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    7128..7369
                     /estimated_length=242
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2131 a         1628 c         1505 g         2019 t
ORIGIN      
        1 tgcattaggt aaagtatcaa ttccaccctc ggctgcagca gcatcagcag tgaatactac
       61 cgtcacaaac ccggaccaac acaccaacat cactgcaccg tccttttttg catcaccctc
      121 gtctttgcta aaaagcgcca cgtcgtcacc gtctccggct tccagttcta acaatagcct
      181 taagtcttta caacttccac ccattgccaa cgctgggccc gctcaagttg ttactgcggg
      241 atcaatggtg attctcaatg gctcaggtag cagagcgcct agtggcataa ttctatcata
      301 ttcctggaat cagatgccga ccaacgctaa aattacttta agtggagtga acacaccggt
      361 atgggagttt actgctccta tcgtctctgc tgatacgctc ctgcgatttc aactgaccgt
      421 taccggcaac ttgggacaga cagggaccga caccgtaaac atcctcgaca aaccgggttc
      481 cacatttaaa gctccggttc ggactcaggt aatgaagtca ccttctgcca gtgcaaatat
      541 cccgcccttt agtcagaata agggttcgaa cattgttagt attcctacta acaatgcaac
      601 aggtcctagg cctttgactc caccgatacc accacctttg actccaccga taccaccacc
      661 tttgacttcc caataaattc tcggcttact ctacaggcaa ataacggaga caagattatc
      721 cacctatcgc caaatgtagg gaatgaccaa gtcgtaattg ctaattcgac cgtcgcttta
      781 gttggtagct tgagcaagga cccagacgca attcagtggc agccctcctg cggagccagt
      841 acgccactat gggagtttta ctgctcctaa tgttgcttct gatattacac cttcgttcca
      901 ctcactgtca ctgatagtca gggcgtaagt gatagcggca gggtgaacgt tcttgttaag
      961 catctgcacc cgtaaatatg acagcgggtc gcacggatca cagcactaga gcacaattat
     1021 atagtcaccc cgtgggacat tgtaatgaaa acattaaagt cacgcgtaga tactgttata
     1081 tacgtaccgc atcttgccac ttcgaatctt cgttatgagg gccacgctga tgcattctga
     1141 ctctaagtta gctacgactg taccattttg caaaggcagt aactgctttt gtgcggtggg
     1201 aaagtctaat tttattgtcc tttaactgtc caaaactcaa ttcagatcca gtcgggatga
     1261 aaatcggatc gtagccccag cctggaccgg caactttggg agagatcttt ccgtcggttt
     1321 taccgagaaa acgtcggagc ttattcccgt cattgtacac aaagactgag acaaaagagg
     1381 ccgatctatc ttttgaatgg gacagcagtt ttagaattcc actactgccg atagttttaa
     1441 agacaaaaga cgaataggag cctgggaaac catttaatcc atggattaaa agcccgtcgt
     1501 cctcaacaat aactggtttg gatattaatt taaaggcatc tttggatttt tctacagcaa
     1561 tctggctaag ggactctcct tgaatttccc ttaacttcat cttcaaaaat ttaatgtcta
     1621 ttttatactt atgcaggatc ggatggattt caatgtactt attagtattg gtgctcagga
     1681 aatatagcga acacattatt ttacaaatat taaaaccgaa ataaaaagtt caaagtgata
     1741 atttcatgat gtatacataa tggaaatcag aggtggcgga caggtgataa aattttatat
     1801 ccaatgagcc gtaccctata tattgtcaac agaagataat cgtagtcacg tcaaaataag
     1861 cataccaact atcgcatcgg attttcaatg ttttgtatgc ggggctattt ttaccactga
     1921 cgaagatagg aagcagcatc tcgagaagga atctctcgga aaattacacg cctcgtctgt
     1981 tcaggagaaa gagattgcac agttgcaaga agccataaat gagaatcgga ggcactattt
     2041 ctgattggaa ttcgcgaatc tagacagact aatcgaatcc tgatacaaaa taggtgtggt
     2101 ttttcgttta gggcatgtgc ttaaatttct tcggctcgtc tacgtgaggc gcatatctgc
     2161 cacgtccctc aatatccaag actttttcca agacctttct cgttcccctt tcactcatta
     2221 catccgaata accattcaag aagttttcga aaagagtttc aaacaaggca aagtgcgcgc
     2281 tgctataaat ttcctttatc aaccttacat ctacggcttt gtcttcaagt cgttctgagt
     2341 aaaaggataa tccaaaatcg acgagaacta atctgtttcc gacgataaaa ttggaggtgg
     2401 tcagatctcc atggacaatg ttcttcgaat gaagcaatcc tgcgtatctt cccatctgtc
     2461 ttgacaggcc agcgttcatt atgtctttag catttttacc tgatatgaac tccattacta
     2521 tttcgcatgc cgccgggtct atgaaatata caaatggaga gaaaactcca gcggtctttg
     2581 cgactgagag catatgggct tcgtgagagg ttcggcgcct gcggatttct ttatcaaggg
     2641 atttatggcg gtacggcttt accgctctaa gtttcgttat agcttttttt ccataccaat
     2701 tcgtgatgta aatgttagcc tctgcaccct ttttgattaa agtctttttt ctagatgctg
     2761 caatagtaat atagcgaggg catagagcat aaaaacatga gtcctgttga gcctagagct
     2821 ggcgagaaga gaattctaga tgaggactgt gcagaagata atttgaagaa cggaatactc
     2881 atgctaacta accaacgtct catatttgag acaacaaagg gaaccttgtt gacactttca
     2941 aaaaaaaccg aagaagccgt gctggatatt cctctcgaca aaataacgtc agtaaaaacg
     3001 gaaggattca tagcaaccaa gcttattctt gttacgaatg gtgcggtata caagtttggc
     3061 gtctatagca atcgcaagtg ggcgaaggct atcgatgacc agatcaaagc tcataagagt
     3121 tccatctgat catctggtcc aagccacctc tacctcgtcg atgcgccatg attgatatac
     3181 gaacgcctct tcaatttcga tattttttct cgtagccata tattggagca ttccaaccca
     3241 ggcgatctgt gctccgttat ctccagcata ttcggcgggg cagagataga atttgctgga
     3301 gtgtctttta cacgcagttt tgagcatccc tgctaatctt ttattcgctg ctactcctcc
     3361 tacaatcaag aattgtcttt ttgatgtaaa cgaaagcgct cgctcaactg cttcagtgat
     3421 catcgaaaat gccgtctctt gcaaagagta cgaaacgtct ccgcaggcga gctcgctgcc
     3481 ttgatctgcc acagcacgaa tagctgcggt aagcaaacca gaaaatgaga catcatttcc
     3541 ttttaccaca tacggtaagg actgatagtc acctgacgat tgggaagcca gccgttcaat
     3601 cctgtcaccg catggtgacg agaaccctag atgtcttcca aactggtcca acaactgccc
     3661 aattgtgata tcaagcgtct cgccaaagat tctccatcta tgttccgaaa aagccaatat
     3721 catggtatgt ccaccggaaa ccaacaaaac caatggatcg ccagcgccag taagcatcgc
     3781 accaagttct acgtggccaa ccgcatgatt aactggaaca agaggcttgt catgaaaggc
     3841 agcaagggtc ctcgcaatta ccgctccaac cctaaggcaa ggcccaagcc caggaccagc
     3901 tgaatacgaa atgacatcta tgtccttaat tgagagattg gcggctgtta atgaatttct
     3961 gaaaaccttg tccgaaattt gacaatgatg catcgatgca tctctggggt ggatccctga
     4021 gccctctacg ggtttgtaaa catcacgcga gtctgaaact attcgacctc tcagcatact
     4081 gtcagaataa gaggattcta taatcgaaca cccaaaagta tgagctgtgc tttcaattcc
     4141 aaggcagagc atattcttgc tagttctatc ccctgcttgc tgcagaaact atcttgatga
     4201 tatcgttgtt ttttagcttg tgctcagctc cgagtctgtg cttggtccta acatctatcg
     4261 cgtatagaaa accctttgct agatcttcgt gaatgaggct ggccaaatcc cttgtagtag
     4321 aatcttgcgg aacgagccgt gcatctggga gcacgtttcc tttcttgtct gttagcctta
     4381 cttcatcttc tacaggataa acgacaatga atttcaatag tctaaagcac acatgattta
     4441 tggcctcttg cacgccggtg gatccgtatc tgccaagcag agacttcaca agatctaacg
     4501 cttttttctg ggcctcactt aaggaattgc cggattttat ttcgaatgaa ttatccccag
     4561 gaaggtaatt caggacgcca cgttttgcag ccctccttag taatgattcg gcttcggcag
     4621 aacaagcaat aacctcaggt ctattgcttt tgatctctgc aatgacttta ttagcagatt
     4681 gcaagtcagc cttgtttgca gcaattatga ccggtttgct tcttaacctg atcttctggc
     4741 agaactctag aagatcttgt tgtgtccaat ccactggttt cttattgtaa agtcctgttt
     4801 catttagaac atcaaaaaca gatctttctt taatggctag tcctgacaat cttttggcaa
     4861 gaagttgccc caatttttgt acctgattct ctgcctctct gagtaccttc gtccattctc
     4921 ttccgacaat agagagaagc caataatcaa attcctcttc tacgaattta atatcagata
     4981 atggatctgc tgtccctgcc aatacgggct ttccttcttt gtctgtcgat gctgaagcgt
     5041 cgatcacgtg aattaacgcg tctgcttgac gtgcgtcgtc gagaaacctg ttgccaagtc
     5101 ccttgccttg atgtgcgccc tctaccaggc caggaacatc gatcaatctt acgggtataa
     5161 atcgagtacc ttctatacat acagaatgga ttggattgtc ctgaacttca aactccttgc
     5221 atacacattt tactcttgta tgagtcatcc ccacattgga gttgatagtt gtaaaagggt
     5281 aattagctgt ttgcacttcg agttcggtcg ccgcattgaa gaatgtagac ttgccaacat
     5341 tggctttgcc tataagacca acgagcatga ggtacataaa tttctattaa cccgaattaa
     5401 tgtctttcag atctctaccg tacttaccga attaatgtcc ttcaaatctg tacgtaacag
     5461 acgataagaa acagagattt aaataacttc agtcagattt cagctgggat ccaatattgg
     5521 ccggaattgt tgctattaac actactaatg taacagtaag caacaacatt gtcaatctgc
     5581 tgtcgcattg tatgcggatg cttcaacata ggggaaaagc atactggaaa atagtagtag
     5641 caaagaaagc tataagcgga gaagggtctt taccatctga tgataagata tcgttacttg
     5701 cacgaaaaga gaaattgatc ggtaagaacg caatcggata tctatcaaaa agatcgccac
     5761 aatttcatag catgcacaca atccacgtag ttcttgacgg tttttttgtt gacacagaaa
     5821 agcttcacct acacccgctg attggagctg caaaagactc tgattctctc tataagattt
     5881 atcacatctt cacaaaattg ctaatcgaac gacgacagcc tgaaagagct gcagaattct
     5941 tggacaggca cctccgtgga aatttgatat ttaaagtcga tgatgacatc tatgccttta
     6001 ggaacagtag tggtttcaaa ccactagtta ttgcctctaa caaaagcaag acattaagca
     6061 ttgtcgcttc ggaaaattct gtacacacat cactaaaaga catgaagttc aacgacataa
     6121 gagcaggtgc gctgattgca ttgcgtaaag gagcggtcga agaggtattg acaaatttgc
     6181 cacgtgaggg aatacttatg gatccgtttg aattcattag agagtcacat gtcacttcat
     6241 tattcaacaa caaaagtata tatgaaatca gaaaaaatat tggaagagaa caagctgatt
     6301 ttttagggtg taaaatggga caacagattg atactgctta tgcagagcca gactataccc
     6361 gtcccatgac actcggtttt agcattaaat atcaaaattt ctggaaaaaa ttcgagatgg
     6421 cagagggaat aataaaggat cggtacgatg acgcggatca tatgatcgat ttctctgaac
     6481 aggtcagtaa aaacaagtta ctcaccacgg ggaagtctct caaatttatt atccaagacc
     6541 gagttcgcaa taagaaaata gcatctgtgc aaggcacaat ccagaccggc agcaccgcgc
     6601 gagaaacaat ttattatttg cgcaaggctg gcgtgacttg tgtcattata ttagtgagtt
     6661 atgttcctac tgctgatggt aggcaggttg gtctgtatac ccaaaacagg gaattgatcg
     6721 caaacaagta cgttggcaaa gtgtcatcca tagacgaact aaacgaaagt gtaaacaggg
     6781 aaataggagc caatgcccta tattacaata gtccagaaat acttgcgcgt gggataggcg
     6841 tacctgaaaa taatctgtgg ttcccagaat gggtaagatt cttggattat aataagtaga
     6901 ctggaatgat caaaaaaatc gacatttccg ataatcgagc gaacaaagaa cctgacgctg
     6961 tcatagttct tttagaaaac gggaagagcg aaaaccaagg gttcatggta caacatatag
     7021 aattacgcca atatattgaa tcgggcgatc cacacctggg cgagtattca ttgatcacgg
     7081 tcctaattaa gacagataag ggaattgtag aaatgaaata tgacgaannn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcaacggaga
     7381 attgtataaa caggttctac gagcctatgg ttctttgtgg gatatcaaaa accgtaactt
     7441 accacttact gtcatttctt gtctttgccc ctggtagcgg ctggaagcca tctatataca
     7501 gcgctatcgt atatatattg aaatg
//
LOCUS       DAOR01000031           46622 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_8671, whole genome shotgun sequence.
ACCESSION   DAOR01000031 DAOR01000000
VERSION     DAOR01000031.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 46622)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 46622)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..46622
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_8671"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    156..432
                     /estimated_length=277
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1380..1581
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1971..2215
                     /estimated_length=245
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2872..3088
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3358..3567
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16916..17127
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    34331..34461
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    37899..37976
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    38313..38496
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        13132 a         9289 c         9268 g        13177 t
ORIGIN      
        1 ttgcaaaaaa gccaagtgat atacatggta tagtcgtaga cccgatacgc cactattcta
       61 cacgtgacga attaagattt catctcatgt tatccatagc tcatgattca gtagagattt
      121 tagcatcaac gttcacaata gtcacggagc aatatnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnttttactt gacccagagt caaaagagac tagaaaacag cgggggttgt
      481 atcatcggag tgaagactta agataccaaa ttagaaattc gctcaaagta ttgtgtgatt
      541 tgaaatgtga atttccaaac aacgtcgtca taaaattgta tgacgcacga gccccacgta
      601 gtattgcgat aattgacaga gaaaatcccg atagtgcgtg gattagagta gaaaggcgcg
      661 aattggatag cgttcctaat tctagaccaa gcgatgcatg gtataggcga gaagaggagg
      721 ggcgctttga taatttttta aaagaatatg atgcagtagt agggaaatca aaggactaca
      781 agtgcccctc tacttcctaa aataatcgct aaggattgag cgcgctgctt ctggcagtgt
      841 ttcaagcttg cgcaccttcc gttctttctc tatcgcatca tacaattctt ctgaaacaga
      901 aaccaaaatc cttttcattg ccattgatta ctattgatat caataataac atttattaac
      961 gttacgtggc gtagcgtttg aatttgtata taaaattgac gagactgaaa aaataatacg
     1021 cttgataaat tgtgaaagac tcgggttctt ggatcacgct cagcccgaaa ttctctagta
     1081 cggaaaggtg ttaaatagat tctttacagt atttttcctt cgttgatttg ctttattttt
     1141 tctaaaatca gatcgctcaa ttctttggtc attggaacat tcgaaagctc gataagtgct
     1201 ggtaatgcac ttcttttgta agaggctaat gcctcaatca atttttttct tctatcagga
     1261 atccaagacc agtatatttg gctcttaaga ttttggatct cccattgctc ctgatcccac
     1321 tcccacaatt gcgtagactt cctcaatgcc tttacgtgtt ttgttattat tataccaatn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nctataattt tgagctgccc gctcagattc tcggagacaa
     1621 gattttctaa ttcttctagt gattgactct gtatcattat tatagaagaa tgaatgtctg
     1681 ggatttaaat taacggctcc cgatgcgcaa cattcagata ttgcaaagac tataataaca
     1741 aagtcagccg aacaaaagtg agcctccgta gagccatctg ttttgcatta ccatcccaca
     1801 acatacgggg gcccctccgg agtaggttat tgggggacgc gcacaacaag gtatttaacc
     1861 attacatacc agccgaaaca gaagaatcta ctataatatg ttgatatatc taaaatagac
     1921 tgtaagaaat cgtcgggtca tggatgtttg aaatgaaaag tacaaccgca nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacgt
     2221 ttccatttct gatccacact ttaaacactt gaaggatctt acggtagctg taatgtaggg
     2281 tgtggcataa ttgataaccc ctaggaaaat aaaggtagaa attgaaaata ctcgtattcc
     2341 aattatccaa tttggtgaca ataatgaaga atcaccaatt cccttgccca cgggtataag
     2401 cgtcaaaatt aagagtccgc aagcgataaa atatactaca gaagtcaaag gcatggagta
     2461 ccctacaaag tcaaaggcat gaaaaaagga tttctgacag acgcgacaga ggaagcaata
     2521 aaagtatggc tgaaaagtgt taaatcctca aaagagacca atcgctgatt tcttcatttc
     2581 aatgcttcta gagagacctg aatttcttta gcatcaattt cctctttctg catagcttcg
     2641 attaaaagcc ccaatactct aataagctct ctccattgct gccattccat gcggttacga
     2701 attttggcga tcgaagaaat atcagcgaga atttggtgtt ccattaaatc aacgttaata
     2761 ccttcttctt tccactttgg cacattattg gtataaccgt atagaaccgc aattttctca
     2821 tctatagtac tagcggctga gtctcccaaa aaattaagca tgctattaat gnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aaaattaagc atgctattaa tgtctcccct
     3121 acctttccgc atttctaaaa tcaatgcgaa tattgcgcct cctattacag ctgcgctgcc
     3181 tatcagtccc caaaaagtct catttaccat gaaaagaacg agaacgagcg gcaaatatat
     3241 tattaaaatt gtaattccta aatacataat tccaatccat aacgaaagca cccttcatgc
     3301 agcgcaagca cccgcaatgc agcaaccaat ttcaacaccc tcattcgcag cagagccnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnaac ccgcgataag taaccagtac tacattcaaa
     3601 gaaacgttaa taagtcacta gcgatattac tagtgatatg ctatccaatt ggttttcgag
     3661 ggtggggagt gtgattccca gaggattctc aaggcattat gtgctcggcc ttctaaaaga
     3721 ccaacagcta tctggcaaag aaattattga taaagctatt ctgcaaagtg agggaaaatg
     3781 gaagccatcc cctggattaa tttaccctat gctgggtcgg ttactcgagg agggtcttat
     3841 agatgagaca gagaacggga ggtacaaaat tacagccaaa gggcttgaaa tggctggaga
     3901 tatacagtca gttcatagga tgttacagaa gcaagttgac gtgatgctaa gggtggggag
     3961 tgtcggtaag tttatggcac tagatctgat cgacagagtg tctacaatag gagccacact
     4021 tagctcgaac cttgatagaa tgacagtcca ggagcgagac aagtataagc agttcttgct
     4081 gactgaatta aagaaaatgg acgagcaaac ggaacataaa gagaaagtgg ctgtcgaata
     4141 atagtaataa tattttattc actccgctag ctcataaagt cttaaggcct cgaaattatt
     4201 tgtcttctac tgcgatagat gaaagtatgc tgcgaataaa caaacttatc gaaaagatga
     4261 gcgctataga acaagaaatc acaaatgaaa ctgaaatact gaaagaacaa tatattaacg
     4321 ccagctccgc gatgggagat acgcataatt attttcttag tggcgtcgaa tctgctccgt
     4381 cccaaaagtc ctacttgctc acatccaggg ggattgaagt tctaggagag gaggtcattc
     4441 cgattagtgc gtttatagac aatgtcatac gttatgcaat ctcgcccaag aataaaatcg
     4501 aagtcctata taacttggtt acgcacttaa agaaaataga tcaaatgctc tcttcttgat
     4561 aacgtcagtt cagtgtcatc ggggatgtac aagtaactgt gatcatctta tcaatttttg
     4621 cggacttgaa ttatcattat taaccagatt gtcagatagt agagactggg agacgaaatg
     4681 cgaatagatc atatagctat tgctgttaac aatttagatg aagcaatagg aaactaccaa
     4741 agaattctga aagtggaccg tttagaagta gaaatagttc ataatgagaa ggtccgagtt
     4801 gcaatgctag aattatcgga cacgaggatt gagctgatgg aaccaacatc cgaagatagc
     4861 ccaattaaaa aatttctagt cgaccgaggt gaaggaatac atcatatcgc cataactgcg
     4921 gatgatatcg agaaagacat ttctagggcc actgcaaatg gtatgaagat cctcggcgga
     4981 ttgaggcttg gatcttatgg gagaagaatt acttttattc atcccaaatc gattaatggt
     5041 gtcctaatgg aattttgcga ggctccagcg aagggctaga ctaagaacta gtcgaagcga
     5101 cactaattag agttgcattc tggatctgtc cgattatttc taatgttata gacgaaggct
     5161 ctgtagttgg tattaagcgg atgcacatct gcgtcaattt gtggggtaaa tcttcatcgt
     5221 ctttttttgc atagttcaaa ggtcagtcat catatggcat ttttctctct ctcagataat
     5281 ttctcgttta aaagaaatcg aattcttgtg acaccgtcta tttgctcaat gattccggcc
     5341 acagagtctg gaacaaattt tttccagcgg tgtgatcttg acataagatt acgaatattt
     5401 gtaccattgt attctttcct tctactaaac ctaggtttca gaatttcagt ctgacgcgac
     5461 accaagtttg taacaaattc gttgccagaa tacagaatat caaaatgagg taccatagat
     5521 ttaagatgtc caaaccatct agcattattt tcatcgttta ccacaggtac aatgtggcat
     5581 tttgtcaaat ctatcttcga ttctaccaaa gcggcatgta tcatcattat ccgttctcct
     5641 gcagtaaacg gatccgtata gctgtagttg atttgggcgc ttccgatagc aataactagc
     5701 tcgtcgcaat cttcaaagat ctgctcaacg aggcaaagat gtcctttgtg aaatggttgg
     5761 aaacgtccca ccattagtcc gcgctttaaa ttatcgacca ttgtgcatgc caagaccttt
     5821 tttttgttgt tctattatct ctcgttcgat cttgctgaaa ataggttcga ccttgccaag
     5881 tgaatgccca gcagaaattc tcatctgcga ggcagatgac cagggttgta ggtgtatgct
     5941 gtcggtcatg cccaactggc accatatttt ctcacaagaa aatgggataa aaggctctaa
     6001 catgactgct attgacctta cggcgttcac tgcaataaac aacgttgtca tggcagtttc
     6061 tctacgagcc cagggttgtt ttatttggaa gtattgatta aagtgagtag taaatctcag
     6121 aattcttttc aacgatttat cgatttcaag tgccgacaac aagcaaccta cctcttgtgc
     6181 tattcctgca atctgtgaca gactaagatt atcatcggag tcatattctg caggtttggg
     6241 gacctttccg gaaaatgttt tctgggtaaa ggacaacgcg cggtttataa agttccctat
     6301 atttgcaatt agttcgttgt taatcttctc agaaaatacg tcccaatcaa aattgatgtc
     6361 tgtctgggag taaggcgtga tagatgcgag ataaaatctc aaatagtcag gagcgaaagt
     6421 tgatgtaaaa tctctaagac caatatacca gtttcgactt ttcgagatct tttgattatg
     6481 aagcagaagg tggcctctgc tcggtatgta atccggcaat ttgtactctt cgtctatccc
     6541 catccgcatt gctggaagga ttaggtaatg atgatacacg atgtctttgc caataaagtg
     6601 atagatctca gagctgttcc agtatgcttt accatccacc ccagactgcc gaagataggc
     6661 agttagtgtg gagatatagc agagatgatt atcgaaccaa ccatagaata cttttccttt
     6721 ggcctctggc aacggtacag gtatgcccca tgataaatct ctcgttatat cccagtccct
     6781 tagtccttcg tttatccagt taatgacata atttttaacg tcggattgaa gattactgtt
     6841 agtatttagc catgttttta gttggcttga gaaactagag agtctaaaga aatagtgctt
     6901 actctgtttc ttgatgggat tatttccaca tatcccacat cgaggctcta gaatctcatc
     6961 gggaacacgt ccacattttt cgcaaaggtc ggaatactga tcttcggctc cgcaaaaagg
     7021 acattttcct attacatatc tatcaggcag gaatttgtta tcgaatttac aataaaactg
     7081 tattacgtct cgttcgtaga tgtgaccatt ttccgaaagc tttttgaaaa cgtattgtac
     7141 aaactgccaa ttttcttttg aacttgtact agagaaactg tcaaaagata ttcctaacgt
     7201 cgtgaaatct tcgtaatctc gcctattcca aaaagcgaca tagtcgctag gactcttgtt
     7261 ttccctttct gcgttaatta acacgggagt tccaaaatca tcggaagcac aaatatgata
     7321 aattttcctt cctgttcgcc gagaatatcg tgtaaaaata tctgctggca ggtaagtaga
     7381 agcaatatga cctagatgaa tttcaccatt tgcatacgga agagcgctcg ttacgacagc
     7441 actcgtgatt ttctcgttaa cacctatcac agaaaaattt ctcggtttgg atcagatttt
     7501 aagctatccc taacttcgtg cctctggaca attagtagtt agcgatattt ctgttagtga
     7561 catgttagag agcttcatat aaattggaat cagcaatcat caatctttag acctgctgct
     7621 tcgacagcat agtgagccac atttatgtcg atgtcaggcg gaacattgtg gattttgttt
     7681 tccattgagt gataattctt tgcaagaaat aaaattgata ataactggtt ggcaaacgat
     7741 aaagccatca cttcaggtgg atgcccctcg gcaccaacta ggttaataac ccttcctttt
     7801 gctaacagat atagtttctt atcacgaaat ttaaagcagt ctacgttagg ccttaccaaa
     7861 acgggaaatt tgtcctgaga agaaagataa tcgacatcta tttcgacatc aaaatgtcca
     7921 gcgttagcca gtatcgcccc atccttcatt tttatcagat gctcttctct aatgacacgc
     7981 ctgttgccag tacatgttat aaatatgtca ccgagctgcg caatctcgga taattgttta
     8041 acattgaatc cgtccatgtg tgcttctaga gcttttatgg gatcaatttc tgttatggta
     8101 acaattgctc ccatccctct tgctctgaca gatacacctt ttcctaccca gccgtaaccg
     8161 catactatta cttgttttcc tgacagaaac aagccagtcg tcctcaagat cccatcaatt
     8221 gtgctttgcc ctgttccata tcggttatcg aataaatgtt ttgtatatgc attattcacc
     8281 gctatcacag gatatttcag gcgtttcgtt gattgtaata tctctaacct cttcacacct
     8341 gtagtggttt cctcagttcc gccatagaac cgagttgttc ctagtttatg agcagcaata
     8401 tgtaggtcac cgccatcatc tgtcagaata tctggccgag attggagaac gcgtttaatg
     8461 cactcacggt attgcctttc cgattgtcct ctccatgcga aaatccttat gccctctgaa
     8521 tacaaaaatg cagccacatc atcttgtgtg gataaaggat ttgcagaaca aattgcgatc
     8581 tctgcaccta acatttttgc ggtaattact aaaaccgacg tttcttttgt aatgtgtaag
     8641 caaaatccta atttataacc tgataatggc ttcttagttt cgtttctgat cataatcttg
     8701 tctaatattg tcatatgggc cctagcccat ctgtatgaca tttgtccctc cttcgccaac
     8761 atcatatcgg caattttgct ggacaacagt agtgatatct ctctaagata ctagcaggta
     8821 gtgatatacc agtctaggcg tccatgaccc caattgacgc acaagataaa tgttttttat
     8881 tgtcattcag agttttttta ccgaatttaa aatgcaatct ttggttcgaa cataccaaac
     8941 acttctttaa agacgttgtt tatttcaccc agagtcacgc tgcttttcac ggcgttgatg
     9001 atgtggggca tcaaattttc atcaggattc tccgcagcct tttggagtgc gctgatcgcc
     9061 gcatcagcct ttattttatc acggtttttc ttgaaatcct taagccgagc aacttgcctt
     9121 atttcaattt cattatctaa catacttatc ttgggttcga caccttcatt atcttggaat
     9181 ttgttcacac caacgataat gcgttcagcc gagtccacct cctttttcag tctgtatgcg
     9241 ttcttcctta tttcatcttg aaaaaatcct ctttcgacag ctgccaacgc cccacccatc
     9301 cgatgaattt ttttcaaata tttttcaacc tcttcttcga ttgtctgggt taaggattcg
     9361 atatagtaag acccgccaac cggatcgact gttttcgtta cgcccgtttc tgtcccaata
     9421 atctgttgcg tccggagtgc caccttcaca gattcttctg ttggcagagc aagggcttcg
     9481 tcgcgagagt ttgtatgcaa tgattgacat ccacctagta cagcagctaa tgcctgagtt
     9541 gtgaccctga cgatattatt atcaacttgc tgagcggtta atgattcacc gctcgtctgg
     9601 acgtgaaacc gtagatggag cgattttgca ttcttggcat gaaagatatc cttcattatt
     9661 ttagcatata tttccctggc agctctaaat tttgagacct cctcgaagaa ttccattgta
     9721 caacaaaaga aaaatgataa tctctgggca aagtcatcta ttttaattcc tctatctaca
     9781 catgtactga tatattctat tgcgttagca aaagtgaaag ccaattcttg aacagcggtg
     9841 cagccggctt ccctcatatg ataacccgag atgctgatcg ggtaccactg cggtacatgc
     9901 ttgtaacaat gctctatagt gtcacctatg agtcttattg atggctttgg aggataaatg
     9961 taggtattcc tggctatata ctctttcaaa atatcatttt gtgtagtgcc tcttaacttc
    10021 cctggagaat agccttgtaa ctcagctaca ctaatgtaaa gtgaaagcag ggtcgacgcg
    10081 gttgaattaa ttgtcataga tgtactgact ttatccatcg ggataccctc gaagcacgtc
    10141 accatatcct tgagcgagct aatggccact ccggtccttc cgacttcgcc ttccgactgc
    10201 gagtcatctg aatcacgacc tgtttgtgta gggaggtcga acgctaagga aaggcctgtt
    10261 tggccatgag aaagtaagaa tttgaatcta ttgttcgtct cttccgcact cccaaagccg
    10321 ctatattgcc gcattgtcca taaacgctcc cggtacatat caggatagat tcctctcaag
    10381 tacggaaatc taccgggctc ttggtcctga gtctttttcc gtagtgaatt ctttttgtat
    10441 actttcctta cggggatgcc agaatctgtt ttaattacac ttgtcagatt cactcacctt
    10501 agcactactg cagccagttg ttcagcagcc acatatggat caagttcctt atttactatt
    10561 ttttcaataa agtcttgata tctgttatct tctctgagca tagcagaaac tttttcttca
    10621 accatgttaa aaaccatatc tcttaactcc acctccagca ttctcttttc ctgatctcta
    10681 taattgtctc ctttactacg tagtatctta tcaatattaa cagcaacatc tttaattccg
    10741 ctccccgttt tagctgatgc tttgattacc acgggcttcc taggagtgtc gcctatcaaa
    10801 tccgtgatag aattgtatag actcgtcgac ccttcaaggt ctcctttgtt aataatatac
    10861 aggtctccaa cttctgttag gccagctttt attgcttgta tgctgtcacc agtctgtggg
    10921 ctcaagacta ccgcagtaat gttaaccacg ttcgaaatct gtacttccac ttgtccagca
    10981 cccacgcttt cgactattat caagccatag ccagctgcgt caagaatcct tattacattt
    11041 ctcactgatc tagaaattcc ccctactgct ccccgcgaag ccaagctcct aacgaagacg
    11101 ttctggtcat ccatggtagt tcgcatcctt actcggtctc ctaagattgc tccgccggtg
    11161 atggggcttg tgggatcgac tgcgataatg gctatcttat agccaaggtc ctgaaaagct
    11221 ggaaccaatt tttctatcag agaacttttt ccagcaccgc cagcgccggt gaatcccacg
    11281 gtcttagcat ttcctgtttt attaaatatt tgacgcaaga ttatgtgaga atcaggttca
    11341 tcgtcatcca cgatggaaat tgctctagcg agcgagcgac ggtcgccctg aaaaagaccc
    11401 tcaactatag gatgcacagt atctccgata tagatatacc aatattaaac acttccaaaa
    11461 agaatattca agatggaagt cactctaacc cgagctgcat atatgagcaa tagatacaca
    11521 tggggcggcg gtaagtttta aagtctagtt cttcaatctt tcctgaatga ccccggaaca
    11581 acgaaaacct catcaacacc agcaacaaca acatcacaaa atgatcagag tacttgtttc
    11641 aaagcttgga ctggacggtc acgacagagg agctcttgtg atttgcagag cgctgagaga
    11701 cgctggaatg gaggtaatct actctgggct attctgtacg cctgagcgag tcgccaacac
    11761 cgccctagat gaggacgttg acgcgattgc gatgagccta ttgaacgggg cacatcttgt
    11821 tctttttccc aaggttgcaa ggctactgag ggaaagagga gtaaacgaca ttttacttgt
    11881 aggaggggga attattcctg agagtgacaa aaagtcatta gaacgtgaag gaattactgg
    11941 gaatttcggg ccgggaactc ctttatcgac tgtaatccaa cacattacag caaatataaa
    12001 aaagtcctcg gagcacagaa gcccttgaca ataacatgta cgatttggat gctgtggaca
    12061 tgacgccttc gtctagatta aaagaaaaat tctgaataac gggttgccgt tttcaaatcg
    12121 gcccatgtag ttctaccttc cgcaattagt ctaaacaagc gcgatttgct atttgtacga
    12181 ttatgtgaaa ttaattctat tcaataacaa aacgccccat gtctagctcg atccaggctc
    12241 tcagcatttt tattagacta acaagataag tgctgactta tctatccaaa ttctggctgc
    12301 ggggtttaga cacattcaac tcatttttta tcttctttac taaacttgct atttcgatag
    12361 gttttcgaat aaagcattct acctctagag agggaaaaac ttctctcagg gcttcgtaat
    12421 atacttcaaa cgcggtaata aagcatactt taataccgtt atgtatcttc tcaagttctt
    12481 tatacaactc aaagccattc atttttggca tccttacatc cagaagtaat aggtcatact
    12541 tagaagcttc aaaattcgat aatgcttcaa gaggatcgtt aaaagtatcg accttgaagc
    12601 catcgctttc caaacccttc ttgaaagcca aggtaatacc agaatcgtca tccacgatta
    12661 aaatatttgt catcgtcaat cgataaatga gccatacttt tataatttat gaaaaacata
    12721 tagaggtaat tattcggact tgtttaagat tttcgaatgc agtgtaattt tctataattc
    12781 tgatcttaga gaggaaccat agataagttt agaactaaga gctaaaagtc tttcattttt
    12841 caagaaataa caatctttta gcagattttt aacggctagt ttaaccgcgt agagttgcaa
    12901 gaagaaaata gactttaata aagtttctat catgctccgc tttctgcagt cgacgtagag
    12961 agtcgagacg ccgagtttat cgagctggtt catgattgtc ttgctcactc ttaagtcaca
    13021 tgcgttgttc ccgagtataa tgattctatc attactctta atactgaaaa ttggggaatg
    13081 acagaattca tctaatgaat aggctgacga gtgtatacca aaaacctcat tcatctttag
    13141 tgacccgtat aaagcagcag gatatactat gccatttcct aggagtatgt aggaaggaga
    13201 ttttttaatt ttgtagttgg ttagataatc agccaaagcg attgactcat tgtatatttt
    13261 tttaacattg ttcaacttta ccttgcacac taaagaaaga cagcatagca tagaagcggt
    13321 aaaccctatg gtgccagaag ttggaatgct agcgcttttg tatcgcattg aaatgagctc
    13381 gtcagagctt tttgcgagag ggctttctgg ccttgtcgtt attgcaatcg ttttgagggc
    13441 ggacttattt gccaccttag ctgctagcat agtagccttt gtagcgccag agactgatac
    13501 gagatatacc ttccgattcc tcacgatagc gggattcaag acaatatcca ttggatgaca
    13561 acacatagcc ttataacctg acacatacat tgcaatcaaa gacgcgacgt acgagtctcc
    13621 cgaaccagta ataacgcagt catcaatgct agtttgcggt agtggaaatt ctaactcttc
    13681 caagtcttct gtctggtatt ctatttctgc ttccattgcc tcaatcgaac gcatgctacc
    13741 accagagatg gcaagacaga tttattgctt atcagaatgc taacaatccg ctttcaatat
    13801 ttgaatccgt atagacatat ttagccgacc ttcctagtac tagaggtgcc aaattattac
    13861 gatatattgg aggttaataa tcatgcttct caagttgaaa taaaaaaatc cttccggaat
    13921 cttgcattga agtaccatcc tgataaaaat aaaaattctg aagactcgaa gcataagttt
    13981 atgcagatta tcgaagctta tgaagctctg tccgatgaga tcgctagacg aaactacgat
    14041 aacaagacta atcacggcac gcatgattat gcttctgcac gaccatggat tccatccgct
    14101 gactttgatc gaatatatag ctattctgag atcaaaagaa ggtatagaga cggcagcatt
    14161 ggcggtggta tgtgggacat tagtgagagc gctagtgtag ggctatggaa ggcgactatg
    14221 gttttattcg gtggtttggg tgcagtcgtt atattgataa tgatatttca ttaacaaact
    14281 tatagctatt tagtgataat ctggtctttg gctttacaga actcttggtc caggacagac
    14341 cacgcatcac cgcggatgcc tgctcgccct tgcgtcattt gcggactgtg aagttaagag
    14401 atcagcctgt cagtcatgtc agaggcagca aagattttcg gaccttttct tgcgtcctct
    14461 gaaaaatgtg ttttactaaa tttcaaagat gatgttacga gctagatcat ttttaatcac
    14521 aattattagc ttcgcttctc tagagaaacc agacttcaga ttatcgcgta gatcagctaa
    14581 tataagattg gaatatgtat acaagagaat cgacgattga tataaaagca ttcgaaatat
    14641 aagaaagaac ataaagccat tgtccatgat tatagtgagt gtaattattg gatcaacgtt
    14701 agttgcttcc atggctggta tatctttttt tatcatgggt aattcttcgg ccactcacac
    14761 aagctcacac aattttctta ccggaatttc gataccttct aatcgatatt taaaagccga
    14821 tatctcagta aatggctaca agttgattgt agatttggca ctcacccaag atcaacaaac
    14881 taagggtttg gccgttaaga accacatgaa cgagagtgaa ggaatgttgt ttgtttttca
    14941 acagccctct aggccatcct tctggatgaa ggatatgaac tttccgattg atataatatg
    15001 gctgggcgcg aatcgttcag ttgtctacat tgctcctaat ttggagccat gcccgtcaca
    15061 aagcaattgt ccaggatacg ttcctagtag ggattctcta tatgttctag aaaccacggc
    15121 tggtttttca catagacata acgtcacggt tggcacgcaa acgaatttcc aacttgcagg
    15181 atgatggtca gcatttaagg tcataggtca acatccccag aatcgagagt ttttttatca
    15241 tgcttgttgg ctactacaat ccgagaatag ttgccttttg ttatgacaag atctcagcat
    15301 gatttttttg atcagtgtct ctgtctgtta ccctcatatt gctggtcttt ctcgcgaata
    15361 tcagatatgt ggtatgtcca atcatcctca tcgaaggtct gctcatgcct tctcttgcct
    15421 ctatatttcg aatcatcagt tcaacacagt ctacatcaat gaaattattt tgttttagct
    15481 ctgttgcgga cttttcaagc tgattcatag tcggacaaat ggcaacgaat gcaccgctag
    15541 gtttcaacga gcgatagact tggccgatca tggtccaagg gtcgccaaga tcgactatgg
    15601 ctgcgtcgac atcttctatg tcaagaccta ggtgtgggtc gtatagatga gttgtaacat
    15661 atgctgtcat tgcagccttt tctagattgc gcttggcaat ctcaataaaa tccggattta
    15721 cgtcgaaagt gtaaatgtgt ccactaggtt ttacgatact ggccagaaaa gtagtaagag
    15781 ctccactccc agtaccgatc tccagtacct ttgatccatt tttcaatcct gtacgagcta
    15841 ctatatatcc caaatctttt ggatatatga tctgtgtcct tcgttcggat ttcattataa
    15901 agtcgtgaat agttggttcc actagatgga taagttttcc ttcgctggtt ttaacgatgc
    15961 taccatattc taatccaata atcgatgaga catcgattac tccgagatgc gtgtgtactt
    16021 tcttttcact attaacccgc gtcagccatt tttttctcga ggtgtgaaaa agcataacgt
    16081 aggagccttc ctggatcgtc attaacaaaa aataacacaa gttacaagag ataaaaatcc
    16141 ttcttggtgt agagccgtta cacggaaaat tattgtttga taattaaaga tgatatataa
    16201 taacgacaaa agaccacttg acttggtgct gaagctaacg taaactacat tgcagtgcgt
    16261 gatatgtaat ttataaggta tttaacctgt ggttaataca gacgatttaa tgaagggggt
    16321 tatcttcgat ctagacggcg tcttagtaga ttccatgcca atgcattatg aggcatggaa
    16381 gatagcattt agcgaagtag caaatatcga agttgatgaa acgacaattt atttgcttga
    16441 aggaatgcga gggattgaat tagtcagaaa catttttgag atcaagcgga ttggaccgtt
    16501 tgatgaatcg attttaacgc agattacaac aagaaaggat gaatacttca gaaagatatt
    16561 tagtttcaaa ccttttgagg gcatgaagga gttggttaca aacttaagat gtcgtaaagc
    16621 agtagtcagt ggttctgcga aaaaagatgt tgagagaatt ttaggccaag cccttggatt
    16681 cgggaacgag caatttgatg ttgtaattac cgcggacgag gttacgaaag gaaagccgga
    16741 tccatcctcg caattttcag ctgtggagaa actgaacctc aagacaactg aagcgctagt
    16801 agttgaaaac gcacctttag gtgttgaggc agcaaataga gccggtatct catgcatagt
    16861 agttttgaat aatacccctc tagatacgac atatttcaaa tccctaatat cagagnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnngag gagcgaattc tcaaacggac agaatcggct
    17161 ttcaactttc tgcagaagtg gtgtaatgaa tgaaagtgat tatattttct catgaatctg
    17221 atctcgacgg gttattttcg gctgctattg ggttaattcg atatccgcaa gcacgactcg
    17281 tatttctcaa ttacggcgcg gagagcttta taaaaatgag atggttcgta gagtccgcaa
    17341 cccgttcctc ggaggagaag ggtatagtcg tcatttcaga cttagggctc agctctgacc
    17401 ctagtttgat cgatacctgt agagaaacct tttcgatagc caagaaaaaa aactggagta
    17461 ttatctggat agatcatcat ccttggcctg aaaacgcgat ccagggtata gaaccttacg
    17521 ttgagcttgt gttggacagt gtcagttcga agtgcgccgc agagttgatt tatgaaaaat
    17581 ttctgtcagg acaccgcctg gcagaaaaac ttgcaagtct agcccataca atggattact
    17641 ttacaaatga tcaatatcta actcctactt cagaactaat aaaatactat cataacttcc
    17701 ctgattgcta cacccgcctc atcaatctcg cacagaaatc ttcgcatggg atactatggg
    17761 atattgaaat gcaaacagat tacaacgaat atgcgctatt acgagacgag gcaaaggaac
    17821 gcgtactttc aacaatgcga atacgagaca ttcataatat caagatcgca tttatacaat
    17881 catcaccctt cattcagaac agtttatttt cggatgaaat tttcaaaaaa acaggcgtgg
    17941 atcttgcgat ggtttacggc acggatggca gagtaagtat aagacgtaat aacaataaaa
    18001 tttcttgtaa tgatatcgca gcgaacttac ctgaaggagg cggtcacata ttcgcggcag
    18061 gtgcaacttt tcgcagtaat ccaagtgata ttgagtcggt catattggag ttggagagcg
    18121 cagttttgac ggccttggaa ggaactgata cggcagttaa tctgtgaact cgcccaagtt
    18181 ttaatctgtt tcggaaaagg gacagaacat agacgattgg atgattgata atcatcgctg
    18241 gttgatggtg atcttaagta taagcatagt atgtcctcta aggataacag ataaaattta
    18301 gtacacgctc tttgtatttg tttttcagga atagctgaaa caaatggtga aatgtccatt
    18361 acaaggaagg tttcgaagaa aattaaagaa ggtctggatt cgtgtctcat aatggaattt
    18421 atatagattt tgctaatcaa ttggtagatg gagacgggtt ctagaaaaaa tgaatttaca
    18481 gtctaccagt gttgtaagag agggcacact ttaaaatgcg catcgatcgt aaagactggg
    18541 ggggtaatag tgtatcccac cgatacggta tatggaattg gttgcaaccc atatttggag
    18601 gaagcagtcg aaagaatctt tgaaataaag ggtcgaaata aagtcaaccc ttttcccatt
    18661 ttagcatcaa acttacagga catcgaaaaa attgtgttac tcggtaaaat cggtaaccgg
    18721 ttggctaaga tattctggcc aggcgccctt accataatct ccacacttat tgatacacga
    18781 atttccacaa aggtcaccgc aggtaaaaca accgtcggag tccgagttcc ggataataaa
    18841 tgcgcagttg ctctgttgaa gcattgcaaa tatcttgtag gtaccagtgc aaacaaatcc
    18901 ggagagaatc cttctaattc aatctctgaa gtgattttgt cgtcgttgag aggtttcgac
    18961 gcgattttgg acggaggtaa tattagacaa gggagcgcct caactatttt ggatctgtca
    19021 ggacacgtcc ccaagataat tcgggaagga gcaatctcat ccgaggaaat tgccagggtt
    19081 ctttcaacaa acaacaactg atctagttat ggactttaat ctcatagttt caacatatag
    19141 acatcgagaa gacgatgcgc aaaacgaact gcacgacatt cttgaattgt ttggagatcc
    19201 aaaaccaggg tcagacatta ctgacattgt cggtatcttg atagggcgta cggtgcttga
    19261 accatttaac gtagtgaaga gaatcaagga actagtccat gacgagccct ggcgtgtcag
    19321 atacattctc agacttattc cgatagaatt tgtttttact gaggacggac tggattctat
    19381 taaaaatgct gcaaagcgac tcgcttcaaa gatgcaaaaa ttcgagacct tcaggataac
    19441 tgtcgaaaag agaaatactt cattgcgttc ttctgaaata atcgcaaccg ttggttcaga
    19501 aattgacaac agagtagatc tagaaaatcc tgattggatc gtactggtgg agataatcgg
    19561 aaaacttgtt gggatctccg ttttacggcc aaatgaaata ttcagctcac tcatagagaa
    19621 gcgacaaggc gagaccgtta cttagttagc aaggctgctt tttctgaaag atagctagtg
    19681 aaggcatcat catttgataa aaatttattc aattggactc cgagccgcct gcaaataagt
    19741 tctttctttg ttttgtttgg ttttaagaca gagtcgtcta tcttaagatg catattacat
    19801 atacgtctgg taacttttat caatttcttc tttttttttg agaaaagaat aaagcagcct
    19861 ggtgcttgat tccttaagcc tatgtccatt agagctctcg atatttgatc cgtgcaggat
    19921 attcgaagaa gaagatccat ttcgggtctg cctaccaatg tgattttcct tttttctgac
    19981 tcgatagtga tttttaaggc ttggagaatg tgttcgattc catacacagc atctgcacgc
    20041 acaccctgta tcgacacccc tccattcgag gtttttcgta aatcgtcaat aaattcgccg
    20101 acatcgttca catctaaatc ggacactcca acaactgtcg taaatatatt acccactttg
    20161 tgtattcggt tctggtgttt caggaacttc ctctctccta atttcgatgt tgtttaaaga
    20221 ccttgtttta gcggacatcc aatgatctat attgggccag tgcacacact tctacgccgg
    20281 gcgatactta ctgtctcgta agcgaagcta gaatccagag ttcgaatata gatcataagc
    20341 atagtagcca ttttaatatc tatattcctt ttacatgcta aaaagatttt cgagactagt
    20401 taagacctca tgtccgtgaa tcggcgagat gaattcagct gaatcttcca gcttccaaaa
    20461 attaacctgt acgtcgacgg tatgattaaa aaaccaagag ttatcagtcc cttttcatgc
    20521 aggtttcggg ctaccgccat gtacaactgt tacaatagta acggtatctc cactctctat
    20581 tacggtatca ttgccatcaa gtgaggaaga ttcaacacca ttaactgcta tcaaaatatt
    20641 atgaggactc aacacctctg gttccttgac gtcacgccgt aacagtatta gtatttcaga
    20701 taaggagaga gtagactcct ctaggataat tgatggcttg ccaaccgctt ttttcacgcc
    20761 gcctaggagt ctcacgttta tcatgattca taacgcgtct attgtatttc tggctctgtt
    20821 gttccgtctg tagaaccttt ttctgtattt tccctgcgaa gatatgccgt gatgaatcca
    20881 gctattctat ttctgagcag tttggacctt acaacagcta ttttcttaag agtcgccttg
    20941 ttttgatcaa aatctgtacc gaaattttcc ggatatcgct ccaaaatctc agcagaaatc
    21001 cgcttaattc tatccattca ccaaaatatt aacaagacgt aattttatct tattgaaacc
    21061 gcgggataga aaggcgactc acgttggggt cttgtctgct gtcatgtgtg cgcaaaatgc
    21121 aataaaagat atagtatagg agcattgcag actgctcgta agataaaata ggacagactg
    21181 ctctgtaggg cgtatggtgt caagtatccg cttaaacggt gacaaagtcg aatccttgac
    21241 gtctcttgcg aaggattctt taccttggga atcctgtgca ctattgttag gcgaaactag
    21301 cactgagaat ttggacgaaa ttatcgtgag cgacgtgatt actgtgaaaa acagcgattc
    21361 ttcgccagta tctttcagta tcgatccaga cgatcttatc aatgcatatc aagtgtccga
    21421 agctaggaac ctgcaagttg ttggaatatt tcattcgcat ccagcggagc cttttccctc
    21481 gtcaacagat ataaaatata tgaagattaa ccctgtcgtg tggcttatct attcaacggt
    21541 aacaaacgag tcgagggcgt atattttcga agataaacta aaggaagtgg aattgcgggt
    21601 tacggtatga gccgctccgt gtcccgagga tacagcaccg tttctctgat attgtcggaa
    21661 tgagtcagga ccatcattaa tcggtcgaat ccaagtcccc atcctgaatg cgggggcatt
    21721 ccccaaccga atactcttag atggcggtca aaattcatag gatttaatcc ttgatctatt
    21781 aacctcttgt gcaactttac tgggtcatgt tgtctctggc ccccagaaat cagttccaaa
    21841 tacccgtatt gtagatcaaa agactgactc agttttagat tgtctggttt ttcatgtatg
    21901 tagaacggtt ttagcttaag tggccagtcc gtgataaaat aaaatccctt atgtatttcg
    21961 cccagttttc gaagcgatcc atcagagaga tcctcgccga attcgagctt ttctccttcc
    22021 ttccctagtt cttctataca ttgctcgtaa gtgagcctgt ctataggaaa tcttggggtt
    22081 ggaagcttta gctggtcatg taatacatcg ccgtctgttc tatccattat tttgttaaca
    22141 ttaaacgtat tccttataac ttcctcggct agatccatcg cgtcttcgta atttatgaaa
    22201 gcagcttcta tatcaacgct caaaaattca gttagatgcc ttactgtatg cgaattctcc
    22261 gctctgaagt agtcggctat ttcaaaaact ctatctagag ctaagattaa ttgttctttg
    22321 taaagctgtg ggctctgtgc taggtaggct tgcttcttga aatattcgaa cccaaaaaga
    22381 tttgcgcctc cttcgctagc actacctatg atcttggggg tatttacctc aataaaggcc
    22441 tttgacctta aggtcgttct gatattatcg agcacggccg accttagcaa gaaaatcgaa
    22501 gaagtcttgg ggtttctcaa atctagtgct ctggaatcta atcgtttgtc tattgctgac
    22561 tcaaccctgc cagtcggatc gatgggcaga gggtggatgg cctcggtgac aatatttact
    22621 ttttcagcct ttatctctag aggaaaatct tttgatttac tacgttgtac aacgcctgat
    22681 acaataactg cgctctgccg aggtttatct ttggcaacac tcagagtatt tgctgatagg
    22741 acaacctggg ctgctcctcg gatatctcta atcgttagaa aggcgacttt gccgatgtcg
    22801 cggatatcct ctatccaacc tgcgatactc accacttgtc caattaagct ctcctgaact
    22861 tgatcggtgt aatgcgtcct gtggatatca tccaacacgc tcatgttatg gtttcatccg
    22921 ttaagtttaa gctccatatt ctattattaa ctcgacgttt ctaagctcct tggcaatttc
    22981 ttggattctt ctggttttaa tggcgttctt ggggtctttt cggtctatta gtactcgagt
    23041 taacttcttg ccatccggca gccagaagaa gttcatgttc agcactttcg acggaaaaaa
    23101 tagattctcc ataaacctcc tgtcagttgc ttgcgcttca acgaaccaga ctgctcctcc
    23161 aaactcagct tctattttct ttaaaagctc cggattagac ctcagaaaac tgatatcaga
    23221 acccctaaga aacaaaacag agtccactcc agatttggcc gcgctgatca ttgacaatgt
    23281 gttgacctct tgttctcgtt cagcgagtcg agtcaatttt attgctgttt caatttctgt
    23341 aggagttaat tggccagctt ctaatttggc ctcacagcca gcacagagga cgccagtctt
    23401 ggcgtcaaag gaacaaatcg gcgtcttcaa ctattacccc ttttcataaa ctaagttaat
    23461 cgttacgcca attatatatt tggctaataa taggccacga cctaaccgag tcttagtaat
    23521 ttgttagact tgtacactag ggattggatt gtggcctgac ctgtaatgac gaaattgcat
    23581 ggtcatctac cattgtactt gctcctaggc taacagagat tatttggatc ttcagatgtg
    23641 tttgttccac agttgacaag ttatatttgg ctgctaaaat atttctaacc tgagcttcag
    23701 ggtcgatgac ttggtttacc tgcgattcta gtgtaatatc aagcgttttt ccgaccaacg
    23761 gtgctggaag ttttcctgac gtgtcgttta atatagaaaa aagaacctta tccattgtta
    23821 tagcatgggg aagcgtcaat agtgcattcg ctgaataaaa cacttgcacg tttagtagac
    23881 tgggctgatc ttgcaccagg gggcgtgaaa ttactgtggc gctattacct gaaaaagaag
    23941 ccaacccacc tgcatttatc tgaggtcgta tcacaataaa cccttcaatt aggttacccg
    24001 tgttattttg aatgttgaac aattggagaa tatttttgca tattatactt gcagatgtct
    24061 gaggttggag ggtctttaaa atagacgtag tacttttacc attattaaca actgcattcc
    24121 ataaaaaagg gaccgtgtaa ctttgactgt tgaatacact aatgtctgtg tcgtagtgtc
    24181 ctggtctaag tggaccatca tttccagaca cagatccgca ttcaaattta gcgctgtaac
    24241 ttacatcctt tatttgtgaa ttgctgttgt caaagctcct cgcatttaaa ggcaaatgca
    24301 caaatgctga tgtggcagta tgctttgatg tcaactcaac tattcttgac gagccgtttg
    24361 ctatgtagcc tcctggagct tttaattctg ttacattgta tttaccaggt cgcaatcctg
    24421 acatcgtact aattccaacc gttggatcac ctggatcaag tgaattgacc tttgcgatat
    24481 aattactcga actgtcaaat gggtttggtg aaattgagta tgtcgcgccg accaaaggag
    24541 atccgtcaga gcgaatcgta atgagcctaa gagatgcatc ttgatagtga gtgctgatgg
    24601 gctgtctatc aatatggctt ctcttgtcaa cttgattttg agaaagttcg atagacgtga
    24661 aggtaataat gagagctgtc actacaacaa agattgaaat tattattgtg atatttttta
    24721 atctcatcga gcgtgaggta gacaaatagt aatacttgca tcaatttata ctcttggcag
    24781 ataatagaat agaatatgta tggagtcaat ttatggagct tggacttacc ctcgtgttca
    24841 gtcagccgta aacgtttaga tcggttatta tacgcttgaa ataaaatatt attcttgaat
    24901 gacagaacta tcgcaagcta aattatcagt agttggacta aaagcctatt attttgctaa
    24961 taagggcgtg gtgaaggcgg tagataacat tacgtttgcg ataggggaaa acgaatctct
    25021 gggaatagca ggtgagtcgg cctgcggcaa aagtactctc ggatccgccc tcctacgggc
    25081 attgcaacct cctggcaaaa ttataggagg aaacgtgata atcagtggaa tagatatagt
    25141 aaagctgtca gataaggatt ttaataaatc gattcgctgg aaaaaaatcg cgatgatatt
    25201 ccagggggca atgaatacgc ttgaccccgt ttacaagata ggagatcaga tgcgtgaaat
    25261 tatgcaaata catcagttca aaggaaatat cgaggatagt attttagaat cgttagagca
    25321 agttggactt gatcgcgatg tcgcgaagag gtatcctcat gaattaagcg gtggaatgaa
    25381 acagcgtgta gttatagcaa tggcgctact attgaaacca gatattgtta ttgctgacga
    25441 accaacaacc gccctcgacg tgcttgttca agcacaaata gttaatgtcc ttaagaggtt
    25501 aaaaaaggaa aatggtatga cgataatcct gattacacat gacctcggta tcatctcaga
    25561 aattgcagac aagatcgcag taatgtatgc aggtcaaata gtagaaatag gaaaggcttc
    25621 ggatatatac gaaaatccaa aacatccgta cactcaagcc ttgatatccg ccatcccgac
    25681 actacacgat agtaacaagc aaatagagta catcaaaggt aatccgccgg atttgacacg
    25741 gccaccacca ggatgtcgtt tctacgagcg ctgccctcat gcaatgggta tctgtaaaga
    25801 agacccccct gagatcgcga ctgaaacagg gtttacacgg tgctggttat atgtcaagag
    25861 ctgataaaat cctcattgat agtcatgtaa tggtttatct ttatgggcag ctactttagc
    25921 attcacctga ttaccatgat gagtgttaat atgagtattt gcacataaat cgtgcataat
    25981 tccgatatcg cagaattcac aggaagtaac gaagtctaga tcgttgaatc tctcgttaca
    26041 ataagcacat atctcggaac ctaaatcata ctctgacaaa ggttttatcc atgcctcgcg
    26101 ccataaaagc atcatttggc taagtcatag aaatggcgca ttattaaatc tgacatattt
    26161 agaacaatac aaaactatga aaattacagt ttcgcagtct tctgggctaa gaaaccaatt
    26221 actatgttta ttgtctgcaa atcatagtac attgtcatta gtctatgcgt ttatatctta
    26281 aggagttgtt ataataacaa tgcagaagag tgccctcagt gtctacgaga tcagacagga
    26341 ctttcctatt cttaagcgca aagttggtga taataaaaca ctggtctatc tggataacgc
    26401 tgctactact caaaaaccgt tctcggtgat aaacgcaatt cacgattatt attcaaatta
    26461 caatgcaaac attcaccggg cagttcacca attgggagag gaggcaacac ttgcctatga
    26521 gcagacaagg gagaaagttg ccaaatttat caatgcaagc tctacagaag aaataatttt
    26581 tactcgtaat gctactgaag ctattaacct cgtatcttat gcgtgggggc gcaaaaacgt
    26641 gaaggatggt gaaagtattg taataaccga aatggagcat cacagtaata ttgttccctg
    26701 gcagatcctg agcagagaaa aaaacgcaaa actctgctac atcggagtgg acgatcgtgg
    26761 atatctgagg atggaagaat acaaaagctt tctagacaaa agcaatgtgg ggctgatctc
    26821 gctgagcgaa atgtcgaacg ttttgggtac catagtgcct ttaaatgaga ttataaaaat
    26881 ggctcatcgt gatggtattc cagtacttgt ggatgcagcg cagtcggtac ctcatatgcc
    26941 ggtggacgta cagaaaacag attgtgattt tctcgcattc tctgcccata aaatgcttgg
    27001 gccgacagga gtcggtattt tgtacgtaaa acgaaagata ctcgaagaaa tgcctccctt
    27061 cataggtggt ggggatatga taaaagaggt tcacaaacag aacactgtat acaacgactt
    27121 accgtacaga tttgaaggcg gcaccccaaa tatagcaggc gtcattggtt ttggcgctgc
    27181 cctcgattat cttaacagaa taggtatgaa tagagtacga gaccacgaaa tcgatattac
    27241 cgaatatgca atgagtgctc ttgctgatat caaagatgta atactctatg gtccgatgga
    27301 tgcaaacgac cgtggtggag taatatcatt taatatagcc gatatacacc cccatgatct
    27361 ggccactatt atgaatgaac gtggagttgc aatccgttcg ggtcatcatt gtgcacaggt
    27421 gttaatggag agactagatg tgcctgcaac ctctcgggca agtttttata tctataacac
    27481 aaaagaagag gtcgatatat tcattgatgc acttcataac gcacggagaa tattcaagat
    27541 atgagcgatg atatttatcg tgaaattatt ctagaccact atcgaaatcc taggaataag
    27601 ggaagactac ctaaccctga tgtcagcatc cacgatagta atccgctatg tggcgacgaa
    27661 attgatatgc atctcaaagt tgaagaagac aaaattaagg atattaaatt tgaaggaagg
    27721 ggatgtgcaa tcagccaagc gagtgcctct atgcttacag aaatggttct aaataagcca
    27781 ttgaagcatg ttaaggattt ggtaaaggag gacatcttgg aaaatatagg gttgacaaac
    27841 ttaggaccag caaggatcaa atgtgcgtta ctctctctga aagtgctcaa aatgagcatg
    27901 gtgaagtatt atgctgatag agatccggag tcagccagca agtaccaaaa cgaacctggg
    27961 gcatattgaa atcgtatcat aaaatcagag gacatggcag ttggacacta aaaagaaaaa
    28021 caatagtcgc catggcatgt agatatcact aaagtctgaa caccaacatt aatttattgt
    28081 gtagaaggca acgactgacc ctgctattta tcacaccact catcccgtcg tgcctctgtt
    28141 tcggtagagg gcatttactg taaacaatta aaatgaataa acccataaga tgctcgatgc
    28201 agctgtctcg agtctgggcg cattggataa tgaaaattag gtcatttgaa tttataatat
    28261 tagcaattgc gtttgtttgc ctttcagcta tggttgcatc tggattaact actagttttg
    28321 acaagattat aaacgcacac gctaaggatg ttcaaggaaa tgagaagctg gatatactga
    28381 tgatagccgt tacgtcgttg ggcgatctct ctaccttagt tatcatcggg atcgtcctta
    28441 ccattattcg tcgtaccaga aaaatggggc tcattttctt gataagcatc gtattcatat
    28501 cgatttcaat aatctaccta aagcctgtga ttggtaggca agcccctcca aatgagtttc
    28561 aaacgtcttt ggtttttcct aaatactttg tactagaaga cgatagtgta gcaccattcg
    28621 cacgcggcga ttcgtatcct tctaatcatg ttgcaatcgc aactgccctc gcttttgtcg
    28681 tgggctttca aattaataaa aaatccagaa tcggcggttt attaatatgg tgtttcccag
    28741 cgctgattgg catcactaaa ttatacttga tgcaacatta tttcaccgac ataattggtg
    28801 gtttcctctt gggattgatt atttctataa taacgagcaa tgtgatgcat cttgatcagc
    28861 cgtttctgat gtccagattt aaaggaaaag aggatatgga tactcagcag tagctaaaga
    28921 tgggcctgac tccgatgcaa ctatggcatg cataaatgca accccatgaa aatcccaatc
    28981 tttgaaataa taacggctga caaacgacac aacacggaga gtagttaatg atgcttgact
    29041 gacgatcctg ttaaagcgtc tagattaatt aattttatat cttacagaca ggaggcaaaa
    29101 actgcttatg cttatttttt tcgacgagct ttatcacctt ttgtaccttt tcatcgccga
    29161 attttgtacg atgagattta tttcttttca aatctctaag tatttcatct atcacttcat
    29221 aagttaatcc tatctcggct tccgctgttt gtcctttcca taagcctggg ctgctttttt
    29281 tctccaatat gttaggtgga atatgcatat atcttcccat ttccctaacc tctgttttat
    29341 acaagcctgc tattggaagg atgtcggcgg caccatcgcc atacttggta aaatatccta
    29401 attctaattc acttctatca gaagttccaa tcacaattag ctttcttatt gcggcataat
    29461 agtaaagtat acacattctc agccttactg caagattccc ttgggcgaac ttgtttgaag
    29521 ggagttgtct taccaagttg tttttagctg aaccgatttc gatcaactta tgatttatct
    29581 tcaaatcctt agccaaacct gcagcatctt ttctttcgcc cggatgtgga attgttttgg
    29641 aatcgggcaa aattagcccg aacactcgag aaggccctag agctttgaca gccaatactg
    29701 cggctactga agagtcgact ccaccactaa gtccgaggac gaccccctcg gacttgcgct
    29761 gtttaacctc gcgaacaatg aaatcggaaa taattttaga agctttagat ggttgcatgg
    29821 tttccataac aggtgataat tatttaatta tattgggaat tttggctcta ggttgttttt
    29881 ctatattcat atgatcttaa ttgataaaac gtctcgaaaa gaaagcaaaa taaaaatggg
    29941 taaacttcca acgtacagct cacaacatta atttaaatac aaacttgtag aactacatct
    30001 agatggatac tagaaatatc ggcttgagaa atatagaggt tgctgataca aagatcagtt
    30061 ccatagacgg cgaaaacggc aagctaattt atcgcggata tgatattttg gatctggtaa
    30121 accattccac ctttgaagaa acagcctacc tgctcttgtt tggagagctc ccaagcacaa
    30181 gaagcttggc agatttttct ggtcgactta gggaagctcg tagtctacca gaaccgacta
    30241 tcaaaactct gatgactcgg cctacacgag cacgtcctat ggatgtcctg cagtcatcta
    30301 tttccgagct tcctgactac gacataaata tggatgatga tttgaaggaa gctaatatcg
    30361 aaagagcaat tgttctcatg tcaaagattc cgacaattgt tgcggcgtgg gatcggattc
    30421 gaaaagggtt gcaggttgtc gatccaagag gggaaggatc acacgcggcg aattttctct
    30481 atatgttgcg cggtaaagat gctaacaagg aagaatcgaa gatattcgac atctgtctta
    30541 tattgcatgc cgaacatagt ttcaacgcat ccacttttgc agccagggaa atagcctcca
    30601 ctagagctca tatgtatgcg tcgatcggcg gggcagttgg tgctttgtct ggtgaactac
    30661 atggtggtgc gaatattcag gttatgaaaa tgctacttga gataggagac ctaaataagg
    30721 cacaagagtg ggtggaggat aaattagaac gaggtggaag agtcatggga atgggacatg
    30781 cagtatacaa gaccaccgac cccagagcag atgtcctttc aagtttgtcc cgaatcgtgg
    30841 ctcatcaaac gggtacaaag tggtatgaaa ttacagaacg tgtcgagaaa gcaaccaaga
    30901 agtatatgct tgaacaccat agacagccaa tatatccaaa tgtcgaccta tatagcgcat
    30961 ccttatacta cagcatgggt atcccaatgg atcttaatac tccaatattc gcgatttcaa
    31021 gaatctccgg ctggtgcgct catgttattg aagaaaagtt tgcagaagct gctccgaagc
    31081 ctgctcttta tcgaccgcag gccctatatg tcggaaggta ttgcggcccc atgggatgtg
    31141 aatatattcc catcgataat cgagctaagg agctagatgc ttaaccttat ccaataacaa
    31201 taatatcgaa aataataatg ataataatga ctcttaaata acgcttatga tattagtgaa
    31261 acatatctct aatactttca ccagaacatg acttttaaat ctacatacat tctagatcaa
    31321 tagatttgaa aatatctatg ctgtccaaaa cagagagtgc cgttttaatg gatctaatta
    31381 gagaaaagtg gcccgtagat gttatcccaa aaattaagac cttcaaggta tatcaggtcg
    31441 aagaaggcaa atttttacta caggctgaga attttttcgc aattcaaaca cgtgaatctg
    31501 tgatcttacc atttctgggc aaacctgatc tgctagaccg cttcccttcc gtttatgtag
    31561 atatgggtgc catcaaattc gtctgcaacg gagctaaagt gatgagacca ggaataacaa
    31621 gttttgacaa cttcaagaaa gatgggattg ttactgtcaa agatcagacc tacaagaagg
    31681 ggttggctgt aggcctggct cttgaagata gcgaaattgc tgcctctatg tcaaacggat
    31741 atgtgataga caatttgcat tacattagcg ataaattctg ggaggcttac aagtcgattg
    31801 cacagtagtt cagtaatatt gaagactagt tgtgggtcga gtaagagatt tgtttaaacc
    31861 aaagggtccg tggcataatc tccaatcgcg agcgacccca tagctacaat aggtatcgaa
    31921 tgggatgaaa cgcatttaga tgatcctcta gccggatcta acattccaat ggtcgttgat
    31981 cggcgccgtg ctttagatag accagcctat aaggaaaagg acagatagta ataccagtgt
    32041 tacaatctcg ccaaacgtat tttaattaat ggttggatct gatgccaaca gactagttct
    32101 caccttggac ttcaggtaag caacaggggg atttgttacc cgttgattca taagaatttt
    32161 aatatcttca atagaatcaa gatctttcat aatcctttgt gataaaaaaa ctttgatggg
    32221 aattccgaat tgtgtagctg ctcttatatg cgcattaaag ctatcttcgt cgtaatgcgt
    32281 cttcaataat cgtgacggct tgcgtagaag agcgttggat ccgtcatagc gtatagaagg
    32341 actaataacc acacatcgtt ctggtgattt cgctttcctg cacataatat taatatcagt
    32401 agccgttaag agtgggagat ctactgggat tactattgta gcgtgtgctc tgttttcgga
    32461 actatagtca tccgctacgg cgatagcagc attcactccg tgatctttgg tctctttcaa
    32521 aaattttgcg tctgttctcc tagcgatttc cattgctcta tcatcgcttg aaactacgac
    32581 tatatttgat attgctgacg acttttttag tacgctaatc gtgttaataa gtaacagctc
    32641 ggacaatttt attcgttcat tgatacttag tactgctcct agtcttgatt tactactttc
    32701 gaacttctta accggtacaa gagcaaatat tttcatagtt aggcagcctt catttgtgta
    32761 agaagatagg atgcgagtct gttttcatca tcagaagttt tcatcagaat gtctgtttcg
    32821 aagaccttca tgccaagtct tactatcctt ctagatgaat ctccatcatt tttagaaata
    32881 acaaacttgg ttacgaactc agaataatgt tttgctaccc caaaaggaga tgaatttatc
    32941 ctcacggctt ccatgtactt tacggcgggt ccgcttattg cgctgttccc aattagcggt
    33001 gaaactgcaa cgattttttc tctctctgca acaagagctt ttttgaaccc cctgatggca
    33061 agaatcgggc cgatactcga tatgggattt gcaggcgcta taacgattaa cgtggactgc
    33121 cttatagcct ggatcacctt cgggtttatt ctggccctct ctgcaccttc atacctgata
    33181 cttacaactg gtggttgagc ccgatgcttt acccagaatt cttgaatgtg catctcacct
    33241 ctgtcggtca agacttttgt ctcaaccgga tcatctgttg caggaataat tcttgatgag
    33301 accgcgtact tgtctctcat ccgttctgta atataggaca gactttttcc actcttcagc
    33361 atgtttgtgc gtaagagatg catggcaagg tccttgtctc ctagcttaaa ccaagtttgc
    33421 tcgccgagca tctccatttg acgaagacat tcaaatgaat cattttttat gccccaacct
    33481 tgtctaagat caagaatgtc agctaatcca taaatcaccg tatcgatatc cgggcaaata
    33541 taaaggccgt aaagccaaat attgtccgcg acatttgaaa ctactgttat atcgctgaat
    33601 tgcgaggcta ggccacgaat taacttaatg gaaccagttc cccctgcgag aacggtgatc
    33661 atggctaacg tgctatgatt tagtacataa tgtataattt tcggatacat tacggaaaac
    33721 cgaaggttat gtggagacta gcattaacag atttactaag aagccacaat atcatgttca
    33781 ttggcaatca aagatggttg gtcagaacag acaatcaata aggattataa acaggacacc
    33841 atgagacttg ttgtgtgaat attagtgcag ttctgctagc ctgcattatc tcaacaattt
    33901 tagttacagg tcttataagt catgcattcg ctgtgcaact cacagcgttt ctttcgcctc
    33961 cggccaatag tgcgcaacct gatcttacgg cagtaagatt ccttacattg aattacgatc
    34021 ctggcagtgc attagcgcag caatttaacg gtaaagcaga acatgtgaga ttcacggtta
    34081 acggaactgc tgggggaatg agtcagttga tatcaacatt caaccaagat atcgcaactg
    34141 agaagcagag ccctgtccga ttcaacaatg caactttaca atatgaagga gatcttatag
    34201 gtgagcctaa ccaacttctg atctcctaca aagtggatct taagccatct atgtccaact
    34261 atgttctaca ggcgaatgga acccaaggct caattattga tttagattgg aggagtgtat
    34321 cagttaacca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34441 nnnnnnnnnn nnnnnnnnnn ngggttgttt caggttacac atccgcaatt ggggcaacag
    34501 ctgttgaatt cgccggcggc ggctatcatg caagatccat tatttaactt ccaagcaatc
    34561 ggacaatcta tggatagctg gcacttcctc tttgatccta ccggaagtat cgcgggttcc
    34621 gctggcctat tcaatgcacc ggccgggtcc agagctgttt cggtatatgc tctgggcgag
    34681 agtagcctta gagaaggcac atttactgaa acagttaggg atgcttctgc aacgataggt
    34741 ggccaatcag tcggcgttca tgcgtcgact cctcctccca gtgcacagat tcagattcct
    34801 ggattctcta ggacagaaaa aacaagcacc ggtgaggttg ctttagtgac cacacaagca
    34861 cctcaaggaa cagttactgc taccggaggg tttcccattc aggttctgct tgtcttcggc
    34921 ggaatgatgg gcgcagttgc catctttgtg cttgttaaga caagaaagta gtactaggca
    34981 agggccatag gcagtgaagt aataatcgtc accacctgtt tgtctcaagt attgctatta
    35041 ctacgaaagc agcaatctgg acccgctgac gacctgactt tagtctaact tcatcgtttt
    35101 acgacagtgg aggggagacg aatgcgaggt ctgccgaaca ccaactgcct cgcatttgag
    35161 acttaaaatc taaaataata tttagaataa taacgagtct ttatgccaat tctactctct
    35221 taataataga tcctcacaga atattccacc ataaatgatc aatcgtcgat cggcggcctc
    35281 gttcagaaaa tttagatcta ggtattttta ctgctttcgg caatcagacc ggttcctgca
    35341 attctgtgga tccataatgt gaatggcaat tgattaaatc cgcgaagtac aaaactcggc
    35401 ttagaccaat ttaattctgc cgcagcaggc aaaaattact cccacgcccc tttatgttcc
    35461 catttaccag ttacgactta tcttgtaaaa gaactggttt ccagtttatt gaatgtagtt
    35521 ctcatgtgtc ataagcctgt aaaaaaaatc accgaaaccc accagataaa aaattaaagc
    35581 gctgctgact cctgtgtatt gaaccaggtt gtatgagtgt gtgtgctagt agtaaccctc
    35641 cttctgtttt atgcgggatt ccgctgagcg gccaacccga gcagatgcag gatccttatg
    35701 ctctgtttgg ccttgctccg ctcgggtcgg cattttcatt ccattcctat gagcctcagg
    35761 ctattcacca tcatcagtac cccatagctg gatatctttt ctgtcccgtt gccagctgtc
    35821 tccagctaat tatcgctaat tcgagcgcct gcaatgaggg aggagtttcc tctctttcga
    35881 gtaacccgcg cgctagctca catctctgcc taaccattct aagtatatta agttagatga
    35941 ggaaaatatc ctttctcgag ttcaacaatt tctgtggtgt tgaccgcgtt ggtataatcg
    36001 cttaaaatct ccgagtttaa ttcgaaaaac gtatgtcccc atttaaattt gtccaatata
    36061 ttttctgaca cgtgtctcca ccctaggatg aaaagagatg ccgccaatgc ttctgcggta
    36121 gttaacttcg ataatttgct ataattggtg ggatttcctg ccaacaatgc aggcaagcgt
    36181 cggcggacac cattctttaa tgcttttcct ttcagtaatg tatctatggc cctttcccaa
    36241 gagcagtcga ctgcacaaac tgatttgaag agagttctgt catgggttga tagaacgatt
    36301 ttgcaaaaag gatctaatat caatgtattt ggccggataa atctaacaga ttcagccaga
    36361 ccgaacttaa ctaatttcgc ggcagtgcat ttagcagggt catcctgctt gtacattagc
    36421 acttgcgctc gcatgataca cctttgctcc ccggtaattt aatatgtgct aaaacacatt
    36481 cgcaaggtcg tagtctctta ttactcggtc agtttaacat gcccgctggg tgtgtgttta
    36541 ttatgagaaa gaagaaatcc gtagcagaac agcaattttt aactgatctg gcaaatggaa
    36601 caatatgcat cctctcggat aggacagcgg aatagaggat cattgcatca gataatataa
    36661 ctaatgattt gtgaatattc acattcaaat tatgtgttat agtaatagta atagtatgaa
    36721 tgctcggttt gatatttaat gcaaaactct aaccacaaga tcgtagccta aacacgataa
    36781 cgtataaacc cattgacaat tgacttgcga attagagcaa aaagtcaatt atctttacga
    36841 gctaaaccca acatccactg aactaattga tcagaataaa agaaacccaa ctatcgaaga
    36901 tctcgctgaa tgaataatta taaaataatc aagagatgtg ctataaccgt cgtggttata
    36961 gtagtacgca tgatcatgta aacaagatcc gtcattaatt aaacagtatt accgaatttg
    37021 ttacaatcgt cttgagcata ccaagaacca ccattgaagg cgaaatgaaa tgtttttttg
    37081 tttttttctt tgagcagcaa ttaaagctgc ctcccaggac aactaaatag ctaatatcga
    37141 caacaaagtg ctcggttggc gagaatttta acaaccaacg aaacaaatgc tcaatctgac
    37201 atctatgaat taactttcta tcggcatccg taaattatac ttgatattgg cgtcattagg
    37261 cggaccgatt caaaatatga ttccacatcc atttcgatca catttccctg atctatgtct
    37321 attatataga tcatatgtat tctgccattt acaatttctt cctcggttat aggggggttg
    37381 cggtagtatt tatcgcagac acgtttaagc ttggttattt cctcatttct tctatcacgt
    37441 ggtggtcgcc tgtacatctc aggaatagga agaatactaa cgggtgtggt tgcaagaaca
    37501 aactgatttg cgaatctgct atatcttgct atcttgcttg cgactcttgc gtagtagtat
    37561 gacagcgggt ctagagcgtg atctggagga acaaatccag tttcaatttc cacgactgtg
    37621 tctccgtttc ctttactccc atgtagatca catacgagga tgtctgtaag ttgcttttca
    37681 acatcgacgg cgtaaccata gcgaataagg tgtctggcac agacaagctc cattacagaa
    37741 tggtttatct ttaccaagtt tttcttatat aattcgatca acctgtactt tacgaaattc
    37801 attcgctgta ccagttcatc tcgttcccca gatgtcattt ttgtcgaaag gccatctaaa
    37861 tctgcggtga aaaggtctac gtccaaatac caatatatnn nnnnnnnnnn nnnnnnnnnn
    37921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctac
    37981 gtccaaatac caatatatca tgaaatggtc ctttaattaa gctgtgcctt tttatagaaa
    38041 aattaaaaat ctaccaaaaa ctgttgatat tttaatatgc ccttggtaaa aaaaataacc
    38101 gtcctcggtt cggggattat gggtcacggg atcgcacaag tttgtgcaat ggcgggttac
    38161 aacgttacgt tacgtgacat agaatcctct ttgttggata aagcattgga gaaggtaaaa
    38221 tggtctctga gcaaactcgt agaaaaacaa aagatcaccc aaatagaggc ggataggata
    38281 ttctatagaa tcacccccga agtagaccta gannnnnnnn nnnnnnnnnn nnnnnnnnnn
    38341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgt cgttgagaga taccgatcta
    38521 ctcatagaag cagtacccga agatctcggt cttaaaaaga aagtctatac cgatgtggat
    38581 aagtttgcgg aagaaaaaac agtctatgcc tctaatacga gcacattacc gataactgaa
    38641 atggcggcat taacagggcg acctgaaagg ttcataggat tgcacttttt taatccaccg
    38701 caattgatgc gactcgtaga gatcattcca ggacgagaca ctaatcagca taccatagac
    38761 acggccttgg atttcgtaat gcgagttggt aaggaacctg tattgtgccg caaagatgta
    38821 gctggtttta ttgtaaatcg aatttttata ccgttggtac atgaagctgc atactgttta
    38881 gagagggacg gagtctcgat gactcaaata gattcagcag taaggtacaa gatgggtttc
    38941 cctatgggga tttttgagct cgccgattat acgggactcg atgtaatatt caaggccaca
    39001 tcagagatgt attctagaga caccagaata gtacacccgc atccgaaaat aaaagaattg
    39061 gttgatgccc aaaatcttgg gcagaaaaca ggcaagggtt tttatgaata cagtggaagt
    39121 acttacgaac gcgtcaactt aacaccggaa gaagcagcaa agtataatcc aataagtttg
    39181 gtggctgttt cagctaataa tgcagcatgg ctaatttcaa ataatgtatg tacaaaagaa
    39241 gacgtcgaca aggcgctcag acttggcatg ggactgaaaa cagatctatt tggcacagta
    39301 gagatgtttg gtgtaggaaa tgttgtggag aaactgcgag agatggcctc aaagtatggc
    39361 tcgcgctatg agcctgatgt ctatctacag agctacgaaa gcaagtgaac ttcatttgtt
    39421 atcagaaaat atttgcaccg cggaacgcgc gtccttagct accagtatag gaacgcgctt
    39481 ccgctcatct aaatattttc cggcgtacgt atctgctaca ccgccactgc ctgctattac
    39541 aacaatagcc ttctttgtca tatatcccac cgccatttct atcaaagtcc cgacgccgcc
    39601 accgatagcg ataattccat ccgcagaact ggctatgatg aaatctctgg cgaatccaat
    39661 tcctgtacag atgacgacat cgcagaatcc attcgcaaaa gaaaattcct cttgtggtat
    39721 tataccaatg gtaagtccat tattttctct cgctcccttg catgcggatt ccataactcc
    39781 tcccagtcca ccacaaacca aaactgcacc ggcacgggct acttcttttc ctacctcgta
    39841 tgcaatattt ctcgcctgct cagtacacct gtctttattg tatccaacca cagcgatctg
    39901 aaacttacgc acattattcg actatttctg tctgtcttat actttatgat gaaagtttta
    39961 aaaaagaagg aaactaatgt agatcggttg aaattccgag accgagtgca ggccggcaac
    40021 atactggccg aaagattgag tacgagtcct tcacttaatt ctcgagatcg aagccaagca
    40081 ttagtgttgg gtattcctcg tgggggtgta atcatagcgg atatagtagc tagaaaactt
    40141 tctactgatt ttgacgtggc actgggcaga aaactcggag ctccagaaaa cccagaactc
    40201 gcgattggag ctattatgga ggatggcaca acgtacatga acgagtatct tatcaaagcg
    40261 ttgaagttat cggaagacta tcaggaacgt gagaaagcaa agcagttagc ggtaataaaa
    40321 catcgcagcg cactctatcc aaagaaccgt cagtacagac taagagatag aatcgtaatc
    40381 ctgacagatg atggcatagc gacaggcgct acgattattg ctgctgcacg ctggataaga
    40441 aaacatagtc cgaattattt gataattgct gtccccgtgg cccctctgca aactgtggac
    40501 atactgcagc aagaggctga tgctgtagat gtgatcctca gccctgaagc gcttaactct
    40561 gttggacaat tctacgaaaa ttttgaaccg attaccgatg accaagttag agaaattctt
    40621 ataggtgaaa cccactttta gatctcttct ttagtatcta gggacatagt aagagttgcc
    40681 gttattcatt ctaggaattc gacacgacaa aatatcagat agttgatttg gaatgattat
    40741 ggcgtacatg aatcattcca acaagtatta ctagtactat agccagtaaa cgtccgttcc
    40801 ctggcaactt ctacattcac tatcgcaggt acgcccgagt attcagtatc cttaaagtat
    40861 agggccgctt gtttggagca tatgaattat catgttaagg atacctcttt ggcagctgag
    40921 ggaaagaaga gaatagattg ggctgaggtt cacatgcctg tacttgtcgc gttaaaacat
    40981 aaatacgaga gcgaaaaacc gcttaaaggc acgaagatcg caggctgttt acacgtaaca
    41041 aaagagacag gcgtactagt aagagtactc cagtcagttg gtgcggagat ctcttggtgc
    41101 ggatgtaatc cactcagcac tcaagacgat gtggctgctt ccctggctat tgacgaaaac
    41161 atttcggtgt ttgcaagacg gggaatctcc acaaaggagt actatgagga tatcaattct
    41221 gcaattggta ttcggccaaa tattacaatc gatgatggcg cggacctgac cgtcgaaatg
    41281 cacaaaaata tgaacggatc caagtccgtt atctacggtg gcacagaaga aacaaccaca
    41341 ggtgtggtta gactacgcgc gttacaaaat gcaggtaagc tgaagtatcc catcatagca
    41401 gttaatgacg ccgagactaa acacgatttt gacaacgtat acggtactgg tcaatcagca
    41461 ctagatggaa ttatccgtgc aacgaatatc ctgttcgcag gaaagaatgt ggttgttgca
    41521 ggatacgggc acgtaggcaa agggatagca aaacgagcgg cgggaatggg tgctaatgtt
    41581 attgtaacag aggtagatcc gatagcggca ttaaaggcaa agttggatgg tttctctgtt
    41641 gcgcaaatga tcagcgcatc cgagataggg gatgtcttca taacaactac gggttgtaag
    41701 gatgtgattg tcggagggga tatttcaagg atgaaagatg gcgcaatcct cgctaacgca
    41761 ggtcacttca acgtagaaat ctccatccca gctatagagg cacaatctac aagcccaaga
    41821 caagttaacg agcatacagt gcaatataat ttgaagagtg gtagcagaat ctatttgatt
    41881 ggtgagggac gattggtaaa cttggcagcg gccgaaggcc atccctcgga agttatggat
    41941 atgagtttcg ctaatcaatt tttatcggta atgaaacttg cggaatctaa aggttcattg
    42001 aaaccactag tatacggcat agacaggtcc caagacaacc aaattgcgct tgccaaattg
    42061 gaatctatgg gaatccacat tgacgccctg acagacgatc aaaggctata cttggaggga
    42121 ttcagtgaag gaacatagac cttagcacag cacagacatg ttactgctaa cacggtctca
    42181 agatattaat acccacatat tgtttcatgg ttgattcgtg aggggcgttg attccagaag
    42241 tagttggatg ggtggtctag cctggttatg atacctgtct cacacacagg tgaccgagag
    42301 ttcaaatctc tccccatcca ttatacctaa aaagaggttt aggacaaaat taatgttgca
    42361 aaaaaaacgg cagaagtaat agcaaggaaa attttggtta tattcataga tttgttaaga
    42421 gcaaatgcgt aatcatcgaa ttgtcgatta cccattactt tgatcattga ttccgatgat
    42481 aacaactcaa agccagccga ggcggctttc tcaatagact tttttgacag ctgaagataa
    42541 atttctgcaa tatcgtcatg gttgcttgtc ttgccgaggg cgtagtatcc ttgccgcgtt
    42601 ctctttccag aagtagaatg tgtatctgaa gtacagagtt caagtgtttg aattccattc
    42661 cgagataact tggtaatgat attatctcgt agactattat ccatgttatt cgaatcagcc
    42721 catccaatga catattgatt gccagtgacc tcaaagacaa tagttgcgag tcctgattgt
    42781 cctagttctt ccattaatcc cgtcgtgttg caggaaatat catccaagtt ggcatatcct
    42841 actttgaatc catatctggg agcattaatc aattctttca agcagatttc tcctgctgac
    42901 aatagactct cacaatctga ttttttcagc tcctctccca ttgcgttatg actgtcaacc
    42961 acaagaatat gcttcaggcc gagatggaaa gaatattttt ctaaggctag tcgtatgctt
    43021 tcaggcacat cttccattcc cacgggtgcc atggagagaa taactatggc ggtgtcgcca
    43081 aaaataattc cagttgctgt gcttcttcca ctcttagtct ggaatggaat agtgcctgtt
    43141 tcgcaccttt ctgaaaccgt tgcgtgagaa agggttcgaa tatagttatc gacttctgac
    43201 ttggagggga tatttcgtga atgattagac acaccgtgga gaatcaaggc atttctcgag
    43261 tattctgaat atagaagata cggcaggtta ctacccccta tcggtttgaa cggaccggga
    43321 tgcaagtctg gaagaataat agatacctga gcacttggcg atttgaactt tgcaatatat
    43381 gtaatcacct tttccttatg agatcgagcc tcagcaatct cttccattct gtccgagcta
    43441 ttttctgtcc aggcagccag aaatgcttgt aagacattaa atgtgctgtt gacagagggg
    43501 cttccggcct tatcagccaa ccgagcccat gccagagata ggattatgaa aattgaacca
    43561 taagcaaatc ctggggtcat tgacagcata gattgataga aacccaaagg cacaaaggcc
    43621 aacattagaa ggacgggctg aatgaatgcc gaaagtatag atctagggat gtccgtaccg
    43681 aatacagaca cgaaaatccc gatgcgcata ccaactgcta gtagcattcc ctcaactaca
    43741 taattagtgg gacttgttgt ctttgaaagt aatgtgtgaa aaactattcc gagaattatg
    43801 gttagggccc aaagcagaga cgcgaatgcc gagacatgaa aaacctttgt gattttattg
    43861 agggaccttc ctcgcagagc ataaaaatcc agaaagactg aacccgtcat tgcggctaga
    43921 ccgagaggga gatgaagcgc tagcattgat aggtctgtct taaatatatt agtataagac
    43981 aaaacaataa caacagctgt tccgagcagc gagatgagat atgatatctt atacgatgag
    44041 ggatttaatc tagtaaagga ccatcttcta tggataacag atacgctgtt cgaatctgac
    44101 gtacgtcgtc agccgaccgg aaacaaaatc cttatcaaga tagatatacc gatgaggccg
    44161 cctgtgacac cgagaacgat ctcaggcttt atcttaactc ctttggtctc atcttcaaag
    44221 aacctcaaca atcctgcact tgaagctggc agaggagcgt tctttttctt gctcatgtaa
    44281 cccggagtgt cgatagtcgg ctttaaatac attccgttta ggctaattgg ttagcctgta
    44341 aagtattttt gtcattaaag tccatcgtaa atgtcttcag accgatcgta gaagtttcag
    44401 gtttattctt attgtcgttg tattttaaag atagaaaatt aattaacctt ggtatcttgg
    44461 gtcttcaaga gctgcagtct tctccagaat ccttagatag atttcaatga gatccgcttt
    44521 taacttctgc gccgagatat cattctcgtc cttgagtagt tggattagaa tcattatttt
    44581 ttcttgaatt atcgactttg ctaggcctct atttgtattc ctattcaagt ttcctttatt
    44641 atcagctgat tctgttagag tggactgtcc gacaatatcc tgtggattgc agccatttat
    44701 gcaaatattc tcattctgga acctgacttg gacgccgatg cacgagggac aaggatcgtt
    44761 aataagggtc ccccctttca aaagcaaaga tgctgcgtct tttaacttac ctgaattctg
    44821 cttgttatta tgtcgaataa ccattgatgt tggcatacaa tacccaatag cgcaatatct
    44881 tttttgctat aggcattctt tatctaattg actctcttaa cgtcacacag atcattatgc
    44941 acactagttt caagttaaaa cgagagcggg caaggcttaa taagaacagt tgggaagata
    45001 gtttactaaa agatggcggt tatatgcaag aaatgtggct tgccggatga tctgtgtgct
    45061 tgtggtgaac tggataagga agacactcga gttgtagtcc gcctagaaac gaggcgattt
    45121 tctaagacga ccaccatgat tgaagggatt gatccaaaat tgagtgatat tcaacgcatc
    45181 attaaggaac taaagagcag atttgcttgt ggcgggaccg ctaaagacgg tttcattatg
    45241 ctccaaggtg accataggga tgatgtgaag ggatacttgg taaagcttgg ctttaatgat
    45301 gctgctatag aagtgcaata ggcgagcctc acagtaactt cctaacccaa ggaaagagca
    45361 accaagttag ggcagatcat cgaacctgct ctgctcggac ttgtaggtga ttattgggca
    45421 actagataga ttggtcgagt aaaaatggaa ggaagaccta aatcgatgcc tagagtttaa
    45481 ggtgcagttt taagccttta taccgatttc taatagtgac ttctgttact ccagctgctt
    45541 cggccacgtc cttctgtgtc ttgttctcac cgttcatcac acacgctaca tacaaggcag
    45601 cagcggctag ccccattgga tctttgcccg ccgaaatttt accttcttcg gctctcttta
    45661 gaatatctag agctttgcgt tttgtctttt cagacaaacc ggctttacta gcaatccgtg
    45721 cgacacactt caccggatcg actactggca tcttcaggtc cagttctcta ataagtaacc
    45781 tgtaacaacg cgcaacatct tttttcttga tattactagc attagcaata tcttttaacg
    45841 tccggggagt ttcagtgtca cggcatgcag cataaagtgc ggcggccacc aaagcagaga
    45901 ttgagcgtcc tcttaccagt cccttctcaa gcgctttcct atacacatac gcggcctttt
    45961 caatcacggc atcgccaaca gcaagtttgt cctttaatct gtccaactca ctaaatgcct
    46021 gtctaaaatt gcggtcgaca ggctcatgca cctgactcct gctatcccac gttcttaatc
    46081 tttcaatcgt actcttcatc gatgcagaca atggctttcc tgatgcatct ttgtctactg
    46141 gaccaattat ggtcgcaaga cccatatcat gcatagcaag cgatgtcgga attcctgcgc
    46201 ggctcctgtc ttcgtgttct tccttcgaaa aggcacgcca ttcggggccc atctcctcaa
    46261 tcctctcggt aactacaaac ccacagttgc cgcaaaacat ctccccacta gagttgtcgg
    46321 taaccatcgg accctttcca caacgtggac atcggtcgct aaatactgaa atatccctta
    46381 ccaaatttct tcaccgtaaa atttcttcac cgtaaaaacg gcagaattac acatctcata
    46441 tatatatttt tttctgttat ttatggatta ccatcgagta accgtaatgg ttcttcggat
    46501 tgagagattt ttagtaagtg agctttagat tgatgttata acgatttata atcaataaat
    46561 aatgtaaatc ccataaacgc cctccaggta gggagcaatg gagacataca tacctgtctc
    46621 tt
//
LOCUS       DAOR01000032           10138 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_65057, whole genome shotgun sequence.
ACCESSION   DAOR01000032 DAOR01000000
VERSION     DAOR01000032.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_65057"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    210..448
                     /estimated_length=239
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2952 a         1857 c         1923 g         3167 t
ORIGIN      
        1 tgtcgttgag ctactggatg cactaatatc tcatctgaat gcaataagaa gtgtctttaa
       61 ggttatgata ctatcttctt tcattcttgc cccactctct ttaatgtttg ctgcagtcct
      121 cgtaattcat cctttcttca ttcaaagaat tctgttcagg ttccccgacg taggagtctt
      181 tctcttgttt tttattggag tttcaataan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat tcttttctaa ctgggacaag aaatttagta
      481 ggttcacctc attgaaaaat cagcttgata aagagctcgg cgaataatag atagaatgat
      541 agatcagtag tacttaaacg gatgtgtaac actactcata ctcgaatgtt ttaacactaa
      601 cactatgtct agattaacag aggaatgtat agaatgagcc gcttgacaat atggatacac
      661 cctatcagtc attagtgtaa ggacggagtt gccattgttt ttttattcaa atcctgagcg
      721 ttaatgattc aaattcatta gaaaaggctt gagaaattaa gtgccaacga aatgaaaatc
      781 agccttctag gatctaagtg tgattggttt tcaactattt ccgtttacct agttaatgga
      841 gctacttctt ccattctatc ctgtatctgc aataatcgca atcctttctg cattgtccta
      901 tatcacctgc tcacttcgca ctccaataaa gcattttctt gcaaggccaa cagatattct
      961 ttcagctgat taaaatatag atatgtacat tagcttcgtc ctgcttggtc ctcctgtcgg
     1021 tccctgcctc cgctagaagc ttccagaatc gccttattga tatttagaaa aaataccgtt
     1081 aaggtacttc aactaaaata cccggtgggg tgttaaaaat ctttgaacta ctgacagttg
     1141 gtgagtttgt ttcaacgtta atgttaccga tactactgct tccggtgcca aaaacttgca
     1201 gataacagtg gtctacttga attttgagtg gttcaacagg ctcatgagtt acctttgaaa
     1261 tgcagctata gaaattgttt acctgcatat caaatcccga attacgtgta tttactgttt
     1321 cctgaatgac agtgcctttg ttattgtgga taataggagt gacgctgaga gccccgccgc
     1381 caacattact actcgcgaca gccaaagctg agataggaag ataagcaggg tttgttagga
     1441 gaagtagtgc ggcaagagat gcaacggtca aaaacgacaa cacctgaatt gtgatgacac
     1501 acattattgc tctatgaatg ggatatattt tttatatgaa ttaagattta cattttaagt
     1561 tcctgtttat agaatattgc ttcaatgcta tgttgttgga ttttattctg tctctatctg
     1621 gacctgaagt taggggggta agtaagattg ttgactagtg taaatatatt attgacctgt
     1681 ggaacgatac gagaggtagc ttaaataagt agtcacaagt catgctgtta agaatgtgaa
     1741 tgtagattct ttaaaatgct gtgtacttgg tctctgaaca aagttaagcc tattcttccg
     1801 gcatatggtt gtccttttgc gaatgattct gccagattcc tcacaaagcc catttctact
     1861 tttggcggca tcggtggttc aaacggatca acatatgcct caactattgt tggttttctc
     1921 tctttcatgg cttgatgcat tgtagatttc acttcagatg gctcgcttat agcatatcct
     1981 ttgccaccac aagcttcagc aaactttacg tagtcgatag gactgaactc aactccatat
     2041 tcaggatttc caaggaatcc catttgttcc catcggatca atcctaatgt gttatttttt
     2101 ataacaatta ctttgatagg aagactgtat tgtacagcag tcgcaaattc acccatcagc
     2161 atgttcagtc caccgtcacc gacgaacgct acggattgtc tctctggaaa cgctatctgt
     2221 gctgcgattg cgtacggcaa accacatgcc attgttgcca gcgtacctga gagtgaaaat
     2281 ttcataccct ttctcaaata gatgtaacga gcagcccatg ttgtgtttgt gccgcagtct
     2341 acagatatta ttgcattttc ttctagctca tcagaaactg ctactgcaat aacctgtggt
     2401 tttataggct tatcggttct agtacttgtt tctttcatca acctatgcca atttttcata
     2461 ccttgttgtt ttgactttag aaattcaaga tcatactttt gatttagaag cgggagcaaa
     2521 gctgctaatg taagccttga atcacctact aagccaattt caacaggata ccgaagacca
     2581 atcttttcag gctttatatc aatctgaatg cctcttgctt gacctggctt tgggagatat
     2641 tcgatatatg gaaaagatgt tccaaccata agtaacgtat ctgcatcact catagcatca
     2701 ctggacggtt cggttccaag taaaccaaga ccgccaagac tatattgatt gtcatcagga
     2761 attgccgctt tacctaacag tgctttgacg actggagcac ataacttttc cgcaactgct
     2821 agtacctcgt ttccagctcc aagtgctcct tgtcccacaa gtataacaat cctacttcct
     2881 ccgttaagaa ttttagctgc cttttcgatt aaagatggac taggaatcgt ttcggcaaca
     2941 taaacgtctg atgtgtggta agcaacatta tgtctggaga attgaccatt caatttcttt
     3001 tcttgcacat cgattgggat tgttaagtgg ctcactcctc gttgtgccag tgcagttcga
     3061 catccaatgt caactgccat ttctgcttgc tctggaacgc atatcatgtc attgtagact
     3121 gcaacatctg aatatagctg taataggttt acatcttgtt ggtaatttga attcattaaa
     3181 tcagaatacg tggttcctgt gatagcaatt acgggagtgt tatctgcctt agcatcatat
     3241 aacccattta aaagatgtat tgcacctggt ccagaagttg caacgcatgc cccaagtttt
     3301 ccagtatatt ttgcatatgc acaagccata aatgctgctg attcttcatg tctgacgaga
     3361 acaaatttaa ttttgtcctg gcggagtctt aatgcttcaa taaagccatt tatcccatca
     3421 cctggcaagc cgaaaattac gtcaactttc caatcaagca aggcttcagc tacaatatca
     3481 gccgtacgca tactctcata ttcagtcatc ctacacagta ctatagagtg gaagatatta
     3541 ataatttgta catcggatag gcggatttct caattgattt gtttcacggg tatttttctt
     3601 ggttaatcct tagatttaga ttaagaatgt attgaaagtt tagaaaaacc caagttagct
     3661 caaaatgaaa tggcggatct atgctgattc gtgatttgga cgccatgacg ataaggttcg
     3721 tatcgcacgt catacagctc ctgtcgaatc gtattctgtg aggtgcttaa cgagacatga
     3781 accgcagcaa atgcagttca aagataaagt tggattcgag ctaacaccac accatgaaac
     3841 taggaaaagg tctttaatgg agatactata gagatatatg catcctatag tacgccaata
     3901 ttatttgaat acaaaagagg atgccattaa tcttcataaa acgttaagag gaaagatcga
     3961 aattcataat cgagtctcta ttgagactgc ttttggtgaa gaaaagggag cactgccttt
     4021 gatatatact cctggcgttg catatgttgc tgaagaaata agtaaaaata aagaattgat
     4081 ttatgattac acttcaaagt ggaacaacgt agccatagta tgtgacggaa cgagggttct
     4141 tggtcttgga aacattggtc cggaaggagc acttcctgtc atggaaggaa aatctgttct
     4201 gttcaaagtt ttgggcgtta ttaatgcaat tccgttatgc atttctacaa ccgataagga
     4261 ggaaataata agattcgtta aggcgattga accgggtttt ggagcaataa atattgaaga
     4321 tatagaatcg cccaaagtct ttgaaataat aaaaaggctt caagatgaac tatccatacc
     4381 tttatttcat gatgatcagc acgggacagc tgtgattacc ttggctgcct tgataaattc
     4441 tctaagacta gtagaaaaaa gattcgataa cgtaaaggtg gtcatatctg gggcaggttc
     4501 tgcggggtat ggaattttca agatcttaga agacgcaggc tgcaaggata ttgttgtaac
     4561 agatagcaaa ggtgcgatat acgaaggaag ggaagacctt caaaatactg atcctaacaa
     4621 ttcgtacaag caggaaatag caagcaatac caatcaaaga aaactagtag gaaatttgca
     4681 cgaggtaatt agagaagctg atgttttcat aggagtatct ggtaagtctg gacttttaag
     4741 taaagacatg gtacgatcaa tgcacagcaa tgccatcgtg ttcgcgctaa ccaatccaga
     4801 tcctgaaatt cttccatctg atgcccttaa agcaggcgca cggattgttt gtaccggacg
     4861 ctctgatttt cccaatcaag ttaacaacgc tgttgtattt ccatcagttc ttagagcttt
     4921 gttagatacg agagcaaaag ggttggatga acagatgttg gtaactgcct cttacgcaat
     4981 cgcctcacta gttgaagaga ctcacttgaa agaggactat gtaattccaa aagtcaatga
     5041 cccaaggata cttcctattg tgaccgagac tttgaaaaag gcaattagaa gccattcatc
     5101 taatggcaat gctgaaaata aagatcgaca tgacctaagc gatgccatta gccagtaata
     5161 tcagcttcaa ttatctgaaa gcgagagaag attcgtagtt actgcaaatt atggtcctct
     5221 tcaacgtctt tttgggtgaa aagatacatg acgaggatac actattgtct tcctagtgtg
     5281 tagtgtatgg tatatcgaaa ttgggttgat gaataggtta ttggatacca ttgttcttct
     5341 tttcgaagat tagattaact tcagccatta aggctatgta gaatcccact agcgctactc
     5401 ctattccagt atcaggtcca aaaataacaa tcagaacact tgtattcagt attgtacatt
     5461 gccgttggat ctctagttgt aactggttgt gtaaccgtcc gatgcacaat tgacgatatc
     5521 tggctatatt tgcaggggat acaacggagg aagaataaat gtagatcagt attccaatca
     5581 accctattgc tagccaaatt tgaagtactt ttattgtatt caatctatat cagatatttc
     5641 tttgtgtcat aagtattcag ttatcttact atttagattt tgtttctata cgtccgaatt
     5701 attgattggg aaataatcga aaatggtaaa tagacccgat agaatttaat cttcgattag
     5761 aaaggattac taatttcgac ttaatttatt attagaattt atcatgaatg gcgtagattt
     5821 ggtcgataag aagagttgcc tcattttatt ttctatcatg ttccaattcg tattccactc
     5881 gacattgacc agaacaaaac tcatctgaac ttctgtcatc acctacaggg tcaccacagt
     5941 ttttacaaat gcttttttta ctcatatatt tatcacaatg ctttcattat tacatctttc
     6001 ggctctgttg ttggtttaag cgtagacggt tattatcatg gctgtccctc tctctatcgt
     6061 taatgataaa atggccgtca tgaattgctt gaccattcct ctacttcgtt tgaaatttaa
     6121 ttcgacgata ctcagatgcg aaaaattatt agaatagtgt tcagtgcttt cgctgcaata
     6181 tgttacctta tctgggaata atgcccacaa tcatcccaaa tatgtaaata tggcatctat
     6241 actaggaatt taggacaggg aaacaggcat gaatagaatc ttagttgtgg acgatgaacc
     6301 cgatattaac ttgctcctta tgctcatctt ggaagatagc ggatataaag tggatgtgta
     6361 tgacgatcct atcctagcct tatcgaactt caaaccaagc tattacaatc tagtaatatt
     6421 agatataata atgccaacaa tgaatggttt tgtattttat caaagaataa gagaactaga
     6481 tattggaatc aaagcttgct ttctgacggc gagtgaaacc tctcaacagg agttcgaaaa
     6541 ggggatatat ccgatcgtcg cgaaggaaga gctattagtc cgaaagccga ttcgaaatca
     6601 agatctactg gaaaaggtaa agaaaattct aatgaattcc agttactatc ctattctatc
     6661 caaaaaccaa gggtagaaaa actcctacgg ttaactaact gttgtaccta cacaccggta
     6721 atggataaac cgctattctt gaatcaggtt taagagcatg cagactatta aggatcgatt
     6781 taataataat caagttataa cttgaaacct aactttaacg ataaaactct aacctgcata
     6841 tggcatttaa acttggacat agtttggaga tatttatttg ttggtgttgt tgttgtcagt
     6901 acatttgatc tgtgactgtg aatttgctac gctctacttg tttagttttg ttcattttct
     6961 cgcatcagaa atcctacccg tagtgaagac aatttaggta ggacttgtag ccgaatcatt
     7021 tctactacat tttatccatg atcgaacggt tcaggatata tctccaattg gcctcgtgat
     7081 tttgcatcga attgaactcc gtatactgta tatagagatt agttgctgca agtaatcctg
     7141 atctacattt caaacggatg gcattatcgt tcgaattatt aatagcataa tcattcaatt
     7201 aaaagtccat aatattttgc aacatcataa gttattattg ttgatctgcc tgtagattac
     7261 aagcgcacca tggaatagaa cactgactct atcgatcttt ttgtgatatt gatttatgaa
     7321 tagtccgcag ccgacagaag aaagtttttt taaactcctc tagtaagact atataacttg
     7381 gattttacaa tgcgtgggtt ctcccgaata ttggttgcag tagatgggtc tgaatcatct
     7441 atggatgcgg cggattacgc catagatatg gcaaaaatgt acaatgctca gctaaccact
     7501 ttaactgtat cccatatttc aatgtcatcg tatggattgg caggtcttcc agattcaata
     7561 aagcaaacga aagaaaaaca tgcccttgaa tcaaagcaat ggtttgataa gattaatcaa
     7621 cgtgcaaaac aaaatgacgt tcaattaaaa acagaactaa ttgatagtca gatgtctgtt
     7681 gatggaacaa tagcagaata tgcagaaagc catgatatcg atttgataat attaggaact
     7741 aggggaagat ccgggtttaa aaaattatta cttggaagtg ttgcatcagg tgttgtaaat
     7801 tatgccacat gtcctgtaat ggtggtaaaa taattacttc tggctatgcg ttaccctgcc
     7861 ccggccatgc gaatacataa tagcccgcga aggcagaaat cgttttgttc attccaatgg
     7921 ccatccttgt tgtatcatca cgttcttcgc tacgacaaaa aatgctacgt agtggctata
     7981 tggccaacca aaccggaact tttatgccag tattcagagc aagtaatgat agtggaaata
     8041 cttttggcaa gcccgtgata ctgaacagta caagacaatg acaaagaata gtagaacatc
     8101 catcagcaat tcgaattcgt gtcgttgtaa agaatctatt tttacactct ctcatatgta
     8161 acgctggttt ttctaatcca ttcacgctgc ctcacttttt ttacttgttt gctgaaatag
     8221 gtttccactc tggacggctc ccggcttgtt attatcaaat cataaccagt gcctggcgga
     8281 tgttccagat caaaaatctg agagaacaaa attacctctg aggtatcatt acatactctg
     8341 aataactcgt aattttttcc atctgttttg atcaggaaaa actgctctgg tttctcaaat
     8401 gttatatctt ttctactgat tcgtactact cccttgctca tactgcttgc tccaagctca
     8461 ttcttaagcg gctttaccta tgtatcctct tgtgtttgtt ggaaacatat cgattcgaca
     8521 tacttctagc ttacaacatc ttgttaaaag caactagatc gtagcgagct aggaggtgtg
     8581 ttaatcaaat ttgacctcat aacatgatgg ttggtcgcga tgaaattgta tcggtagtag
     8641 tatatacaat ttcgttcatc tactagtcga tgtttattct tctcgtgtat tactacattt
     8701 atcgttgata acgtgatgtg acaatatccc tcacatcaga attttccatg ctcctgaact
     8761 aatcgtatcg agacatctaa tcctgattga ggtattcttc ctttcagaat aagttaaccc
     8821 gacgtttggc gtacataaaa aaacaaccat ttattggacg ttttaactct tagcagtagt
     8881 cgcattagca gtgacagtta ccccggttac atttagacta ttgtgaaatg aggtaggacc
     8941 gtttgcgcaa gagaacttag cagttagctt attctgtcct tctttaatag tagtatactt
     9001 tggagttaac gaaaaactcc actttgaata gtcacctgct ccattaggac caattggcgt
     9061 tgattgttga tatggcttta tcccatttac gattacaaaa acttggcagt tgatagaggt
     9121 aggattactg ttacctgcag atgttccaga aatcactaga tctttcctga caggaacgtg
     9181 ttgaccctta gtgggtgagg taatttttac catgtttggt ttggtttggt gctgtagagt
     9241 aagagtggaa ttggggttac taccagactt tgaaagtggg gtttttggaa atatttgccc
     9301 aaaggctaga ttagtcaaaa cagcagaaca gagaagtagg agaataatag ccccaactat
     9361 gaaatgagtc aaccgtgttg taaacgatct agttccaagt cgaaagacgt ccgtcatact
     9421 aagatcaata tgttatacac aataaaagtg atactaacgc aataaagcta aacctgtatg
     9481 tagagtagat attgctggag tacttttgtc tagatgattg ccattggtct tgttttgaat
     9541 attgctcacg ttacttagca caacaggcat taccgtaaat ttggttaaag cggaggtcat
     9601 ggtatagcct gatgtagata cgtgtattac cagcttatac acgccagcct ttgtattctt
     9661 gtctgctatt aaccatgaat acgaggcagt tcccatgtta tttgaggcta tcgtaaaatc
     9721 ttttgttatt cctgtgttta gccctggaaa aacgatagat cctgttacga tggcactagc
     9781 tattggttga ttagagtttg gatcaaatat tgatattttg atagtttgtt cattccccca
     9841 agcaatcggg tttttagcaa ctttaatggt aatacctagt actttcccag attcctgcag
     9901 agactgtatt gtctttgaac tcaaattgtc attgctatta attatattat tgtgaaaaag
     9961 atttggaaac caaaatgggg aactggaaac ccgtccatca ttgttggaac ttggtatagc
    10021 catcccgtag ttgttagtat tattaatatt attattaata ttagcagggg gatgtattgt
    10081 agattctgat atactgttac ctctgttacc ttgttgattc atattataag tattgaag
//
LOCUS       DAOR01000033            5013 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_26649, whole genome shotgun sequence.
ACCESSION   DAOR01000033 DAOR01000000
VERSION     DAOR01000033.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_26649"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    924..1248
                     /estimated_length=325
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1467 a          874 c          929 g         1418 t
ORIGIN      
        1 taacttgatc tataataatc acctcattaa ttcaccataa tcagcctttt acaaacgtga
       61 caaaaacttt tccgtcattg atcaaggcat cggcttataa tcggtgtagg aagtcaaata
      121 tactgagcga gtctacctat atctatattt taattgcaaa ctgcatataa taagttagtt
      181 tagtcaacga tgatatggtc atgtattatc gtatagaata ttaacttata tacacattct
      241 aacacataaa aaatatgaaa ataaggacat caagacaaat aataaaacca aatgcaaaag
      301 acagccgcta cttctagtcg tatgtcaaga catataccat tgttgtcgca attgagccac
      361 taaccaataa tgcaatatta gccactaacc aataatgcaa tattactaga aaaaagctag
      421 gctgaactga acaaataaat gaatgttatg ggatgggcgc gcccccgcac atatacaaat
      481 gcattggtgc gttaccgtcg ttgttgttag taactatcaa atattctttt agttctgcta
      541 aatcagattg ttcttctttt agtgattgcc agatcgtgtg tatcgcacac ttactatttg
      601 ataggattgt ttcatttaca agcggcgcaa ttggtaagta tgtcttttgt tttagatttg
      661 gattccatgc attgtaaacg aatgcagtcg ctggagatat acccgtcctt gttagttgat
      721 ttggtgtatt tttaatcgtt gtttgattga ctgttggggt tgtttgattg agtccattag
      781 tgccatttgt atggtggagt tcttctaaac gtatttggtt aactgatata gagagactag
      841 cagtacttgg aagtactaca cggtagtctg tagggttgaa aagttctata taggtaattg
      901 tgaagttgcc tgctctcact cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt agcgttagta
     1261 gtcgaggtaa gatttgcatc gtacatacat atgtatcctg gttgggataa tgcagtaatt
     1321 atcttgagtt gagctgcttt aagccttgat aattggcctg caagtatttg cagggatggt
     1381 ttagaattag agtcacaggg gagttttgca tatacctggc cattcattat ctcatatggc
     1441 atagcatcat aaacaggtac gaagtcatga ggtggaatta tctgatgcga taatactagt
     1501 attccaggat ttctaacaaa aatgatggca ttccctgctg ctgatgtttt attattgctg
     1561 ctattatcat ttcttacggt gctattacga atctgattta tggccgttcc caacatagtt
     1621 tttgggttca gaaaagactg aagaatattg gtcgcatcgg gtgaggttag cgcgggtgtt
     1681 ggcttcattg tagccagtcc cttgcttatt gtgttattca cgctatacgg atttttaatc
     1741 gtcgttgctg ctttgactaa agttgtatta ttactgccac ttctattatt gttttgtgcc
     1801 aatagtagtt ggaattggtt gttatttatg agtaagatag atgccgaaag aataatagta
     1861 atcgcaagtg ttacatatag ccatctagtc tgctttttcc tcattactac cacctagttg
     1921 gtctacttta tccatccctt tagcagcagt ttgagaactt cgagagccat caggattcct
     1981 cataatcttc tatgaaatat atggatgggt gtctaaattc ctagttcttt cactgagata
     2041 acataaactc atttctttgc tttctttcta cacctaaaga tgtttcaata actcttcttt
     2101 tgatatctgt gcctgacaaa gaactttttg tcatgaacta aaggatgaag taatctaata
     2161 ataatagtta tttatcatga agttaagtat aaaaaaaagg ttatgcacaa accatctgtt
     2221 ggtaatatct acgcaatgtc aaagtgggag atgtaatgag tgtctcgaaa tacttgatca
     2281 aggggtgaaa tgtggacata gatgccatct aaacacggcg gatattagtc ccaccatggg
     2341 cgaaagttta aggcaacaac aagctgagca agagaaaatt gagacaataa caccaaataa
     2401 acctcacata gcataacagc ttccttcgta agcttagagg gaactattac caattatgac
     2461 tgcagaaata gatgaaattc ggaagcgttg ggcaagattg cgtgtctgca aagtatgcag
     2521 cacaagattt cagagcagca gggaaatgag ggaacacaag gcaaatcatc attcatattg
     2581 aggtagctac accaaactaa agttccatta agttaatcgt gacttttcct ttctagtctt
     2641 gaattcttca actaaaacct gaaatcgaga ggcacgacac gcaaaataaa taatagtata
     2701 taatctatca atttgttgac ctctaatgag ccatataatc tctaaccctc atgttgaaag
     2761 agagatgaat gacaattttg tttgcttact gtattttcgt gacctcaatg atcttgatat
     2821 tagaaataac ggaatggatc cttttacaat atgggataga ttgggatata gagccttcga
     2881 tgacagtgtc gtacctataa ttcttgacca gttgatttac aacaatgtag ataggggatt
     2941 tattcacttt aatactgcaa ctcgaagagt cttacttaca aacgaaggaa gacaatgggc
     3001 tgaagacaag tgtaggacaa gggcaggagt tctaggctga aaaggaggga ctgtaattag
     3061 cttctcctcc agtttactat tctattccaa aatcggatga aggattttta ggcaacagga
     3121 tatcctacgc aaagaaaaaa attgaagatt tatgcaagtc ggcacctgta caagagaatg
     3181 cgccaatagt catcgggttt ggtatagtgg aaattgacta taaattcata aaacttttcg
     3241 atgaagccta taaatgcttt ctattgggac tgtattactc taccgtttct ctctgcagtg
     3301 tagcaacaga aagattatgt tatgatatac ttgaaagttc caagataaat ctcgatgata
     3361 cagaactaga taatgatcag aaaaaagcgt tttttaagat accatatact gctctagttg
     3421 aattattaaa tgaatgcgat ttaatcagtg accaaatagc gaaagacatg aagaagataa
     3481 atgacttaag gcaaaaatat gtccatccgt tattagaagg cgattcgtac aaagattcta
     3541 agcactccat aaatcttcta tgtagtataa ttgattcgta tgtgagctta aaaaggtcaa
     3601 tgcatcatat gtgatctcta cgaatgaaaa aaccaatgca atgcgggtcg gattctgggt
     3661 gatagttcca ttgtctgtca ggctagcaga ttgcctaggg tctttgtgta atatccatag
     3721 tcccaccaag aggcaattat tgcgtccttt gaaatccact gaaaggcttt tgtccaccga
     3781 gtggcattgt ctgcataaca ccatgatatc ataatcttgg ttcaatccac ctaatcttct
     3841 ggggaggata tggtgagtgt ctaaatgagg aaccttattc cgagttctct cacatagata
     3901 acataaactc atttccttgc tttcgttcta gattcttcta cttcagtaat caatccttca
     3961 taaaccttta tttagtgccc ttaaacagtt agtacagtta gctaaatgat actcacactt
     4021 catggtgagc ttgaacacaa aaaggttata cagttagaat gttattttga caagtgtaca
     4081 atatgacatg taaggagggc tttaacaaac gcaaaccaat agaaaactga taacagtttt
     4141 tgttcttggg ttagtggcgt ttatggttag cggttcaacg atgacaatga aagtttatgc
     4201 catccaaact ttgccaactt gcacagatcc aacaggacat aacctaccgt gtatgatgat
     4261 catatcgaca ttgccagcac ctccgaatgc gttgcaatgt caggagactt caggtcagat
     4321 cctaccgtgt tcctatacga cgcagaattt gaacaacggt ggacaaatcg tggctatcac
     4381 tgtgtatgta ccagccactt ttgtattcac aggtccaaca gtgataaagg tcgtagtgta
     4441 tgagacaaca acgacaaaga ttattcgtga agacaacgat ggcaaaaggg tttgtcctcc
     4501 gttgactatt tgttcgcctg caccaccacc ctcatcatgt aaagggattg agggtgttca
     4561 tggatgcatt atcttcctac caggaggaaa gggatttcca ccaacgccaa tttacttgac
     4621 gccgttgcct cctggcccac atacgcctgg tttcaagtca ggatggtttg acgggaacac
     4681 tgggagcgac ccatacaatc cctatcctat tggcagtaaa gactggttgg catacaagct
     4741 aggttacgaa caaggagcag gcaagtcaac gccatacact aattgcgagc actatgatac
     4801 atgtccagat gcgaagtatg atcctacaaa cgatccgaaa tacaaaccac agacttatcc
     4861 ttcctcatgt atatcgccac aaggcataga caattgcaat atggcaaaac caactctatc
     4921 atcaccgtca ctagccacag gtctaccgat accgacaaca acgactccac caacggcaga
     4981 ttgtaagaca aatccaaacg atccatcatg cac
//
LOCUS       DAOR01000034            3673 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_25240, whole genome shotgun sequence.
ACCESSION   DAOR01000034 DAOR01000000
VERSION     DAOR01000034.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_25240"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1020 a          793 c          654 g         1206 t
ORIGIN      
        1 tcggagatgt gtataagaga cagccctata actatataca cgtcatgaac ttcagtaatg
       61 tacaaacgtg gtatctgcca aaaaagtgat ggtgatcgga ctgatggctg tccttggttc
      121 atcagttttg gtcggcgccg tttcgctcta taatcgatct tcatcaaacg ctccttctac
      181 tactattgga gttaatcaaa gtgaacttaa cgcaaggtta aacagcatac ttgacttttg
      241 tctgaactct ttaccaaatg gaattcaggc ttgtgataga caactaaaaa acgtcgttac
      301 tgatgtgtgt actgctaata atggaagatt agatgcatgt cacaatggga aagtagatca
      361 atactatgta gctagagccg caggagtaag gaaatctgct attaataaaa tacagtaata
      421 aatcaaaaga ccgaagaatt ccaagactga actgcataaa atcatgagcc gaggttggtt
      481 acgcgatgcg ccgtcagtca gaaattgcat tggtattgga tagcagacga aatgtcgtta
      541 tacatagttt taagtagcag ctaggggggg cgatttcttt attttgatag tatctctgat
      601 attcttcggc tttgtagatg ttgatgcagc cacacgtcag tttgagtcat catgaatctg
      661 cctgatctgt cgttaaacgt ttgagtttca atttctcctg ctgcttcgcg gctatcatat
      721 agcgatgcag agtacatata taacggctta acagacccct accttatgtt tctctttctt
      781 tatctttaac aacatttatc aatcctaaac cgtgacatca tcgctactaa cgtgcttgtg
      841 gtatgagcag cattggctta tcctctttgg tgattactat gactgcattc tagacctcta
      901 ttgacggaat ataacttgat atatctgtac ggggtggaac tgctcttgag caggagtggg
      961 tgttgtcagt accgaaaagg atggtgcccc gacgataaat aaccaggcat ttaatcagac
     1021 gtgtaaacta cgaatttgat aagattgttc atgagaagaa gacggtgtta acttttgatt
     1081 tctagcagaa ttcttccctc tctttcttta gcagatagtg cctccagggc cttcctggca
     1141 tcttgaatgc tgaacttctt ccatacttta actataagat gtttagcaga aaaagaagta
     1201 agaagctctt ggagctcaat ccggttacct ccagtggcgc ctattaattt tatttgctta
     1261 gaataaagag attgaatatt taatttaacg tcagctcctg ttaagattcc aaatgttact
     1321 aatcttccat ttacaccaac agaggcaaag ctacgatccc atgtctttac accaagcgaa
     1381 tttaaagcaa catctgccat tctaccctca gtaattccat caaccttttc tactacgtta
     1441 tcgtaatccc ctatgacata atcagctccg aaatcagtta tccagtgctg attagaaact
     1501 ccaataacct ttgctccaat tttcttacca agttaaagcg ccattatgcc agtattccct
     1561 gaggcaccaa agacgatcag gcagtcattt acttttagtg aagcctcctt taaggcatga
     1621 aaagctgtta aaatacttat tgacgtgctc gcagctaaat cccaatccat ttcgtcgggt
     1681 attttaaaca catttcgttc cggaactgaa atgtactctg caaagccgcc attagttatc
     1741 acaccaatta ttcctccgtt tctgcacagc atttcagatt gatttaaaca catatcgcag
     1801 gttccatcga acactctgct atatacaata gctctgtcac cttcctttat gctcgtcaca
     1861 tgagttccaa tcctctctac cacaccactc agttcagatc ctggaatgtg gggtaaaggt
     1921 tttacttcta ctccacctga cacagcacga taatcgattg ggctacctta acacgtatta
     1981 atacatcgtg atctgatatt tttggttctt ctacgtcgat caactttaaa aattctaaac
     2041 ctggtctttc gaagttggca gctttcactc acttaggatg cttgttattt tatataatga
     2101 ttaatgtgtt tttgtttatt aagatgtgct ttgtacttcc attgcatggc tattttcgat
     2161 caaggaccgc gcattttcaa agggaagaac ggctacgtct tctcgccgaa atggcccata
     2221 tcttttcata tctacaccca taaactgttc cattggtttt aaaaacctga ttacaatata
     2281 ctttgaacgt actcgttttg aaatcgattc gagtactttt ggcctgcctt tggtaattgc
     2341 tagcagtata tcgtttctcc ttctttcaga ctctttttcc gcatccaaaa tgtatttttc
     2401 ttcctctgtg agtttcgaat aatctatcgc agttgcacta gtcgtagtag tagtcgtagt
     2461 agtagtcgtg gtgggagtag atgcagctga aaatgaagat gacgatgccg aagaagctat
     2521 catactttgt tggtcaagca ccttaaggtg tctgatttct agaagaaatt taattgctgt
     2581 agaaatcagt tccaccaggg aatcccttat tcttgcttct attccatcgc atccatacaa
     2641 ttgcagattt tcgagggcag ttgctatctt tgggtaagta ttaaaaggca ccgactgcaa
     2701 tgtcggaatc tggacttctt ttagcagcaa atttactatg tcagtaattg ttattgattc
     2761 attatcatta tctatgttta ggtttattta tgccgcccca tctatcccta tatctctgca
     2821 ccccttatct ctttctgatc tacctctgat tcgagcatta gaaaagattc ttgaccgaga
     2881 aaaaaagata atctcgtctc attatcacga cctattatct ccttatcgat actcatacca
     2941 caggtggctc gggtttaatt tatacacgca acttatacaa attccctcca aacttaatcg
     3001 aagatgttaa tatttccacc ccggaaatat ttattcaacc atgcttagca cgacttgccg
     3061 ttgctccaga tatcttccat tgttctagtt cagattgaaa atatacgttc cacgctcaca
     3121 ccatgcgaaa agaactccca cacttatttt tttggcatct ggcgcatcgc tcttcatgtc
     3181 gtctatgcaa tatgtcggga gaagctcatc ttttccctcc agagaggctc atccaagccg
     3241 attcgactca ccacatcgag taatgtaaca attaaagcgt ggactaattg tgtctctttc
     3301 aatgaactcc tgtaaacttt aagctatatg tttatttact ctggcacgga ttcacaaacg
     3361 aacctcccgt tttcaatcct tacgaaaaga aatctgttgt ctttgaaaat gagagtaatt
     3421 tcattatcgc ctaactctac atctagaagt tcctgacctt ttataccatc gaactttgcc
     3481 atttctgaat tattattctt cttgcttcta tttattagtg caacgctatt agcaatggaa
     3541 gctaatcgta tcctaattct atcttgtgtt gatcttgtat ctcatgatgt gcgtgtaata
     3601 caaaatagag gctcggggac cctctgtttt attattgata tacacttatg aagcctgtct
     3661 cttatacaca tct
//
LOCUS       DAOR01000035            2951 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_87992, whole genome shotgun sequence.
ACCESSION   DAOR01000035 DAOR01000000
VERSION     DAOR01000035.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_87992"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          838 a          602 c          540 g          971 t
ORIGIN      
        1 cccgtttcaa gattaatgta atcccattca ccaattccgt atactaactc atgaaattct
       61 gcctgtatcc cgggatccaa taatttatag acattttctc ccactgaaac tttattcggg
      121 tctgtcaatg aagttatctt ttctgctata ttgataacgt atccaagtat atcaatttgt
      181 gagcttttat catatgtaga ttgaacaact acaatttctc cctcggttat accgattctt
      241 aaagatagtt cagggtaatc ataattgttt aatattgggt taattccgtt tttgatcacg
      301 ttaatcatct tttgtgcgca tctaactgct gtaaggtatc tattaaagcc aggagcaaag
      361 aaagcaatga cagcatcacc cacagatttc agaatatatc catcataact ttcaaccacg
      421 gaggacattt cgtgtgaaaa ggctctgatt atagtaacta gtttatcagc gggcagtgtc
      481 atactcattt tagtcgagcc aacaatgtcg acatagataa taacgagtga tatcttggag
      541 cttacgtagt taagaagaaa ctcctgagca tgctttattg aggtatcgta ctgatagact
      601 gaaaacctat tgtctatttt gactttgttg ttatcctttt tgtcattatt agtcattttg
      661 tttttttact tttcttgaat tttttcaggt atcattaagc taaaaggccc gccagctgga
      721 ttataatgtt ttattccaaa cagtgtctgt tgcattatca tatcttaaat cactaatttg
      781 cactacgccg tacatctgat acgctataaa ttgaccgtta cctatcgcta aagtgttcaa
      841 tgcaggatta ttgccaagat caatatctgc acaactgaaa tacgcaatat ttggcttgat
      901 tgctgggtca agtacagcag taaatgctac aatctgacca ggtcttatca caggaaataa
      961 cttacttttg acggaatcga tctgtgttcc cttcgcatta tgaacagacg ccaaaatggt
     1021 tacattatga aaatcaaaag gactgatgtt ctttgcggtt ccaacaagag ccctgtcgct
     1081 tccagctggc atattataat aactcaaatt tagaggttta tagtaaggcg ttaaaacctc
     1141 ctttgcattc aggataaatg gagctcctga cacaaaccag gcagggtcaa ctgcaaactt
     1201 aaatggagaa acactagaag ggtaaataat tcttccataa gttggttggt tctttaatgg
     1261 ttgtagagag accaccacca acagttctgg cgacaacatt cagtccaaga acaacctcaa
     1321 ctggcatgct gccattgttc ttaacgacac ctacaacgtt aattttacca tcagagcctg
     1381 caaatggtgg ctgcgctatt acatttatcg ggctaccggc atatacgtgc tgcaccgtta
     1441 ctatgcttcc acatgatata cagattacta gcggactgct atgcttgact cagctttaaa
     1501 tttgatgaga gcaccatcta gtctattatc aagcattcgt cttaaccttt ttttatgtgt
     1561 gttttcatcc tttcttactc atccccaaaa gatgcttcgc tttataatgt aggagtacca
     1621 gaccaaaaat gtccataatt gtcatattgt gttcgttata cattaagtca tctaaacacc
     1681 atttatgaga accctaaaag gatagataga tactccctgc tgccccattc ttcagctact
     1741 tgaattaata cttgatataa ctttgtatta ctctatcgtt aagatgttca tagaggatgt
     1801 gcctctaggc ttggcactat gcattatctt ttgattgatt agttaggaaa tatttttttg
     1861 tctgctatca tcctggtatc tttttcggaa atctcctaaa ttatgtcgag gacctgtgga
     1921 tgcatcggtg ccagtataat cgcccattat ttctaagaca tgttcaaaat cattaagttt
     1981 tttgagaatt tcaggggtca acctcatatg tgttacgaag tgagtaccct tatcatatat
     2041 agctgtggcc ataccagtcg tcttcattat gtgagacctt gatgcttccg ggtcgatccc
     2101 ctcttttgta aatacctcca cggtatacat cggcgcgact ttgggatttt cttgctcccg
     2161 caccaataag tcagaaataa gtgatggatc cgcttttgga atatgtattt cgtatagggt
     2221 ttgcagccct tgaaataccc tccttatttc ccgtaattca gacatgtttc tattgcatat
     2281 aattatttaa gctaagtatt aagtatgctg atttatgcct ctgtgtcatc ttttcctctg
     2341 atgcagcatc ccatttatct ttagtttctt gctcaaaggt tccttgttgt tatgcttctt
     2401 ggagaatttt ccatccgtga agagacgcaa agacagacca tttaacctgt ggacgcagcg
     2461 ttcaactttg aatagtaaag aaggagagct aaatgccgca catcaactga ctacatttat
     2521 tggtgatcct gtaagacagg tttttccatc tcctaaagat aagtattttt tgcaggtcat
     2581 catggttcgg aaaactttca gatatataca attgatgcat tcaatggttg gccaattcca
     2641 cttactcaaa actctacccg aaacattggg ttcactggag caacgcattc tttcggtgat
     2701 acggaaatga agttctactg gaacaaaaaa gtgggatggt tatcagcagc ggcctacaga
     2761 atgtccgtgg ttaatagcgc agctgccatc tggggaagag ttggctgtgt tttcaccatt
     2821 agacctagcc tgtccaatgt ttctaaatct gcttttaagt ttggacgcac aatacatgac
     2881 aatgcagtaa cgaaacacag cactttttca gaaaacggtc gctaagccat taaaccagta
     2941 cttggggttg a
//
LOCUS       DAOR01000036            8973 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42766, whole genome shotgun sequence.
ACCESSION   DAOR01000036 DAOR01000000
VERSION     DAOR01000036.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42766"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    251..463
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2620 a         1745 c         1807 g         2588 t
ORIGIN      
        1 atcatcgata gtgaaattgt agagctgttt tagatgctcc actctcttta ttgttgatac
       61 tcctttgaaa cggctattat gatttagccg aagcctttga attttcaaaa gcgggattta
      121 cattgaatct gtatcggcag tgtttgcaaa gatatcgttg aatctccccg ctatccttgt
      181 tatgtcttat cccaaatttc tcaaccggat ggaaaaatga tgtcagggag tatgactggt
      241 actaatacga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcacgc cccactatcc
      481 atgttaaaat tgtaccaaca ctactaccag taaataggag gagcgcttgc ccgggtatca
      541 agtataccaa gattatatgc tctggttact tagctggcgt cacggacctt ccaagatttg
      601 tttacttcta gagcgtactc ttgatatccc agcttaaatt gttacctcag caaggccttt
      661 acacataaga tagactgcca cgaaaagtgg cacttctagc gtactctctt ttatcttaaa
      721 tgactgacct ctaagcttta aacttatttg tgacgatacc ttagacttta cttccacatc
      781 tctagatcct aaaaggcaag catcatccag cggatcaaaa gattcgagat catcacatct
      841 catcttgact aaaccgctct tactatttat cgtaccgggc attcggaaaa tcctatgcac
      901 atccatcgtt acttggggat ctattttaac tcctaaggtt ttagccatct tgccaacttc
      961 ggacttgaaa ccttcatatc caccattccg taagacgata ttgcttagtt taactacgga
     1021 cgattgatca atagctaact tgtcagctat tattttccgc cagccatatg aaagtccgct
     1081 ctttggaaat ttaatcagga aatcgttgcc tgagttatcc ctgcttttac ggacaccaat
     1141 actctctgtc attatattcg ttcctgccaa ataactcact acatctgatc tagcaacaga
     1201 atcaagagga ttgaaggatt tgtccgatac aacaatatgg aaaccattgt ttcctgaaaa
     1261 atacacagac acagattttt cttcgatacc gagatcggtt gttaaaactg caattagacg
     1321 ccgtacctct ttttttaaag caaatataca tttgccgcaa ggaatggatg tctgattcaa
     1381 agacgtgctc ttacaagact cacatacttc agatttggca cttgagactt cgctgcatct
     1441 tgcgcatatg aagtatgagt gtccgagctc acattggaga tgcaaatcct ttgcatcaat
     1501 gtcaaaaata agatctgctc ctaaccattg tttttcctgc ataggataag ttgggaatct
     1561 gtaatatgcg ttggagcaat aaacatctgc tggaacatct cgtatcaatg tagctaggag
     1621 gtcgccccta tttctaaatg ataaatgtcg gatcattcct gatccaaatg gcatgtatcc
     1681 aaattcacgt tgttctaaat gttccggaat ttcaagaagc ttactgtact tgaaataata
     1741 ttctctaaag gcgcttttaa gaagcgcagc gcttttctcc gttgatttat ttccgctcat
     1801 caatttgctg acttgtaatg cctacggcta cgaagattgt cccttgagca tcggcggtct
     1861 tccgaattga attgggtttg atatcccatt acattcttcc gttgcaaagc aaagattttg
     1921 atttagaagt ttctggcaag aagggactga atattttgtg ccgcttcccc ggagtcctgc
     1981 aagatgctct atctggtatt tggtaacttt ttcattatag tcaggggcat tatgaaacag
     2041 cataattatc tcctcgatac tttttccaat tgcaagcata taagttgcaa gcattagtct
     2101 agcagaatgc ggaagatttt cgcctttgtt cattacctct aacgcatgct tgacgcaggg
     2161 aggatagtct tggatcttgt atctatgata ttggttatgc ggtgcaaatt ttatgctcaa
     2221 ttcattaacc ttggttttaa ttgcttcagg tagagttgac agggtcatcg attttacctt
     2281 gttgtaaatc aactcactaa gttcgcttct aatgagtctc acagtctcat cggcatcaag
     2341 atacacaaag cccttatgca caagcctatt gattaatttc caatcctgtt cgtggaaatg
     2401 agaagcccgc atcaaatagt ctgtaatctt aactttgaac agccgattgt cattttcact
     2461 gatatcaaca ttcaccttga ataactcctt gaatatcgtc gacagaagaa gacgcttttt
     2521 ttgttcactt ttctctctca ttaggtcttc agtaaggaat ttctctgctc tcattgcctc
     2581 aaaaagggcg tgtttcttca atataggctc cattccaatc gatttcacca tgataagcgt
     2641 caccaaaaaa gtaaggattt caatttcgta tttgtccaat ttttcgtcgg gcagcccctt
     2701 ggctatttcc ctctccaacc tctccgcagc tctatctatg atgtggtcca tctccggtct
     2761 gtcaaattct tgaaaatcaa aatgagtttc tctaacgtaa tcagacgcat cattcagaaa
     2821 cggatacttt gcgagatcgc cagttccgat ttttatcgac aatctttgat gacatgaaaa
     2881 gtattggcgt actggcataa aaattacatg cacgtcacag aatttttata tcatagggat
     2941 ttggctcaag ttcatatcaa agaaaatgaa tattcgagta ggcttccatg tctctattgc
     3001 tggggggatt tcaaattcag tcgacaatgc attaaagata ggatgtagag catttcaaat
     3061 atttaccaga aaccctagag ggtgggctgc taaaccacta gtaaacgagg atgtaatgaa
     3121 attccgagca aagttggcca aaagtccact ctcgccggaa tcagttatcg tacatatgcc
     3181 gtatcttcct aatttagcag ctccggacgg ggagttgtac aagaaatcgg ttgatactct
     3241 tgcgggtgag gttcagagat gcagtaccct gggcataccg tctctggtaa ttcaccttgg
     3301 aagccacctt ggcaagggaa ctgaagaggg cataagccag ctagtgaaag catgtaatta
     3361 tgctcttgag aactacggta aagatacctc atcatacgtg aatcagtcga aagaaaagaa
     3421 caacaagaaa aatgcgccag tccgaatcct actcgagaat atggcaggtc aaaaaaatag
     3481 cataggtagc aagtttgaag aaattaggct cctgcttgat aagctaaagc caaagggatc
     3541 ctttggagta tgcctagata cttgtcacac ctttacttcg gggtacgact tgcgaaaaga
     3601 aaatgatgtt gagaaaatga tagatcagtt tgattcaact gttgggttga aggtgttaaa
     3661 ggtgttacat cttaacgatt ccaaaggcga tttaaattct agaattgatc gtcacgaaca
     3721 cattggccta gggaagatag gaaaggcagg atttgctgca ttattacatc atgaatcact
     3781 tagaaatcta cccatgataa tggagacccc tgttgataaa caaaggggtg atgttgacaa
     3841 tttaaaggcc gtactggatc tactgcgcta gtagggcgct tagcgatacc tagacaagtc
     3901 gatgcggcta tgcccttgtc tatatgcata tctacctgct tcgctccaat agccttcctg
     3961 aaaggcatcg gtctttagtt tagttagaac acgttcaggc tcagcgttct ttatcagagg
     4021 gaaaactacc cgggagcgta taaccagctt atctacttgt aacatagtca acgtaccgaa
     4081 gaataggccg ctcccatgaa cgttataaac atgccgcttt acggaatact tgcgagagga
     4141 atacattcca cagataacac acgcccaact gcggagcaac gctgtgcagc gaatatgtta
     4201 ttgcaactgc tgatagatct gttgtcatca tattacgatt cgttaggatt ttttggttga
     4261 cgaggtctcc actaaacttc acatctaatg aggcagcaag ccttgttctc tttgcaacac
     4321 tctgatctac ataaattaat caatttggac ctaaaggatg tgttcagaat acattgatga
     4381 gcgccgaaaa tctactggcg gaaatttctt tccatgctag atgttataaa cctatagtct
     4441 ctattgttaa tgtgatattc agcatttact tatcctgcag ttactgcagt tactccaatc
     4501 agagtgatta ctgcagtttt ttgaggttgg cgcatgtgac tagacagcaa gaaaaaaatc
     4561 gtatcatggg tgaaaggaaa tgagagttca ctactattgt ccacactgcg atcagtattc
     4621 tacaaggcgg tggaatttgg aaattcatat caagagacgg cacggtgggg cagggcaggc
     4681 aattcgcaat atggcatatg accctatgga attgcctggc aaaccgttga gaaagaactg
     4741 gcatggtgat ttttccaaca agtctaaatc taccaacaat attttttcga ccaccctaga
     4801 gacgcaaaga tttatccttg aatctttacg taatatgaaa gaagcaaaag atttgtgctc
     4861 tcacctctcc gggcaacaag ctccaggcct cataaatgca acacaaacca tgaacatagg
     4921 tttctctaat aacggtacta ccttgggatt caaaggatac gtctgtgaga aatgcatgtc
     4981 ccttgagatt gcccatatct tcgatgatgt aaaacggaca tccttaagac cgaaacatat
     5041 atgcaatcaa caaaaattat acgaagcaca atttgtggtg gatatccagg gaacaataac
     5101 taaactacgg caggagttag ttggtgatct tatgataatt gttaataata tgatgcaaca
     5161 acaggaactg atagatcttg ctgctgtaga aatcacagat tctgtatttg ataaaccact
     5221 ttacgaagat tgtgttgatc ttgattcttt ccaaccaggt accactgtcg actgggctta
     5281 tgctgcagcc aaagttggta aaattatgat taacaaaatt gagttagcag aatttctgtc
     5341 tacgtttgaa gcaaccttag gattcttccg actaacaatc gatcgaacac agcgtttttt
     5401 ctttgcatat attgcaaaag aattagagcc tcgggatatc gagtacctca aaaacctcta
     5461 ttcagtggac gcagacctta ggaacggtat cggtggcaat atccataatc acgctagtaa
     5521 tatcgctgag aatgacatgg ttcagtttgt agaggttcca tttgcaaagg aacctgttcc
     5581 catgttgaga ttaaatatat ttcgagcatc ggtgaacttc caataaattt aaaggctaca
     5641 gaagacgacg ttgtcgaata agagataacc taggatgaca aactacgaac tgattatgaa
     5701 acttgccctc gaaagaggat tttacttccc aagctgtgaa atttatggtg acgctcaggc
     5761 tggcttttgg gaatacggcc ccagaggggt tggcatgaaa aacaagttca ttgagctttg
     5821 gagaagagaa cttgtcaggc gtgatggcat gctggaaatt gacggttctc aagttatgag
     5881 caaaagtgtt tttgtggcct caggacatat tggtaatttt acggatccta ttataaaatg
     5941 cacaaagtgc aattctacat ttagagccga cagatatatc agcgaaaaaa ctggcgagaa
     6001 cgtacccgaa cgaatgcctg acgaggagat agaccaactt attaagaaac acgaattgaa
     6061 atgtcagaat tgtaaggggg atttcggaaa tgtaagtaga ttcaacatga tgtttagagt
     6121 aggaatagga ccagctgcgg aggaagcgta tcttagacca gagacctgcc agacaatatt
     6181 tgtagatttt gctcgagtgt tcaagacaat gcgcgggaag cttccattgg gcatcgctca
     6241 aattgggaag agcttcagaa acgaaatagc gccaaggcag agtctattaa ggcttcgtga
     6301 attctatcag gccgagatcg aggtattttg taatcccaac aaattagatg atttgccccg
     6361 gtttgaagag gtcaaaaata cagttctaaa tatttctgat gaaagttcta cagtcaatca
     6421 ggttaccgct gaagaagcta tccaaaaagg aatcttgcca aacaaattag tagcatatta
     6481 tttagctctc ctaaacgaat tctattcgaa aaccgggata gatatgaagc gaacgcgttt
     6541 cagacggctg tctgacgagg acaaagcttt ctacgcatca attgcatttg attttgaagt
     6601 cgagacaagt atcggatggc tggaacttgt ggcatgcaat tatcgatctg attacgacct
     6661 aaaagggcat tctgctacaa gcaagcaaaa tcttgaggta atcgacccgt ctgaccagac
     6721 aaaggtctta cctcacatat ttgagctttc gatgggaatt gatcgaagcc tttacactat
     6781 cttggagcat tgttactttc aagacttcga acacgaaaac agactggtct taaaactgaa
     6841 accctatctt gctcctatat tagtaggggt gcttccgttg atgaccaaag atggattggc
     6901 tgcgaaggca gagggaattt atgccgatct aaaacaagac tatgacacat ttctggacga
     6961 gtcaggatcg atagggagaa gatatagaag acttgaagaa attgggactc cctttgcagt
     7021 tacgattgat aagacaacta tggaggatga tactgttacc gtgagagata gggattcaat
     7081 gcaacagaag agaatccaga tatcccagct aaataatttt cttcagggcg cattgaaaga
     7141 ctcttgatac accaccatta gaaacgcatg ccgatagatg tagaacttag agaaggtgca
     7201 caggagaaaa tacttatcag aagtgaacct gactcacttc gattcggaag caggtaccaa
     7261 taaaaggaat taacaagggt aacatatatt cgctgtttaa tctgccttcc tagggcaggt
     7321 ggcgaagtgc aggctttggc agacgtgttg acaccagatt ttccaagtcg tgataggtac
     7381 aaatatgata gatttttcga atcagaatat cagcagtttt ttaatgagtg gttgaggaaa
     7441 aaagtcttga tatgtttcaa atcttgtttt aagagatgga tgtttggttc aaactcttac
     7501 tcttgcaaga ctcacgtact tcagatttgg cacttgagac ttcgctgcat cttgcgatga
     7561 tgaaatatga atgaccgagt tcacatggaa ggtgcaaatc ctttgcatca atatcaaaga
     7621 ttagatctgc tcctaaccaa tgcttttttt gcataagatg agtcagaaat ctggagtatg
     7681 ctttggagca atatacgggg gttacagacc cgtgttgaat gctggatgtt ttaagtacga
     7741 gttgagagtc aatcttttcc atatacgagt ttgtgctcgc cgactctgta tacttcgatc
     7801 ctatgagttt caaaattggc ggcatatgca agtctatgag aattgctcag tttgattgtg
     7861 taatattcac cgaatcttgt tgtctttttc ttgccaaaca gattaggatt atcagtgttg
     7921 cttaaccgtg tcataacttc agttaatttg ggtctaactg aatggggtag atttaatact
     7981 tgttttctaa aatctttagg ctttctaaaa tcccatttcc tgatactacg gttcattctc
     8041 taattctttc agttctgcca agaattcatc tgatttttgt gtaatcattt gggtcttacc
     8101 cctcttgatg tcctgtaagg ctttttcctc ttcaggccac accttatcat ccgtttccat
     8161 gcctttagac ttcaaccaga ccattatggc ttcttcgagt gccgtagtaa gattacctct
     8221 cttcataccc atagtcttat agactgcatc tcgaaatttt gtttcgagtt catcgtgtat
     8281 gtttagatct aagcgtccca taatacgatg aaactgtacg gttatctgga tatatagctt
     8341 tcctgatatc agtaacctga tactcattat gatatactga tatgtggata acgcgacaat
     8401 gactttgcca ggcgctcgat ctgcaatcag aatggagatc tcgcataggc acgcaaacgt
     8461 aactattcaa aggcgaaaga aacttgctcg taggataaaa ttgcacgcaa accgtaagca
     8521 aaattattca agcgcttgga cacgaaattg tccggatttt tcagcgctct tttgaggaca
     8581 aacgcattca cgcaattata tttagtaaac tacacctgaa gaaaatttac cgcgatcaat
     8641 tacagaaagc atgatcgggg aaaatattct gtcagaaatg ttgtagtccg aactacacga
     8701 aatttggaat aaggcatatc gtcaggagag cggctatatc cagagttact tgtgtaaaga
     8761 tagtaaatat tgattctcat ggtatcctgt ctaagaaaat tcctaggctt cagcgaaaat
     8821 tctatcagcc gccatagatc tcgatttcag aggggtatta tcaaagaaag tcgcacgcca
     8881 ttcgaggcaa cttcatagta gattatatta acttctgtag atggataaga aaatttgtag
     8941 acttgctgaa agcatgtgtt gatagtcttg ttg
//
LOCUS       DAOR01000037            6840 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_59524, whole genome shotgun sequence.
ACCESSION   DAOR01000037 DAOR01000000
VERSION     DAOR01000037.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_59524"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2022 a         1280 c         1222 g         2316 t
ORIGIN      
        1 ctgcaatttg cacatttact aacaatcttt gtcgttggtg attaatgcta taaccaggtg
       61 ttgtagaata cgtgaacata tctttttgct atcttcgaag cgatacgtcg agtgctaatt
      121 gatactccgt atcacctagt aggttttgct ggcaatttgg agagtgccct aggtgaatga
      181 aatatgactt ttgcttaagt aaatgttgcc attgggacca tagccctgac aacatatccg
      241 tgtatgagaa ttattgaaag gaatgtgttg gaccgcacca tttcaccatt atcgcatgga
      301 gattctgtgt atcactattt ccacaaggca ttgacgtttt ctacattatg cttcgaaaaa
      361 ggttttataa ttcttttatt tttattggat agtggtaaaa aatgccctta ttatcacatt
      421 ctttggtatt ttattgatat tcctactagg cattacaacc atattctcag ataattttac
      481 ttctgttaga tctatggaaa ttctgacaaa tagtgagcca aggtcggtaa cgcccatcca
      541 gaattctcac tcctttaact gttcatctct acctactttt aatgatctgt ctcctaacca
      601 atcaaaattc aaggctgcga attttactct agattgctat agccataaac ttggcttcga
      661 taactacact caaaatgaaa agaataacta taagaatggt taaggatggg gagaatctat
      721 ttcgaacact tccgatccta tccgatccca tccgagtcga ctacaactaa gtaaagcacc
      781 tcatatcgag ggtaattatt cactacaatc cactgctttg aaatcagatc aagtaaaggg
      841 tggagctcat gctatggtgg ctcttaatga tattaaacat aaaacgtggt tcaataatgg
      901 cgatgaggta tggtatcatt ggtataccat gattccaata gatcaagcca ttcatgatac
      961 atggcatata ttgactcaat ggcattcacc tacaggagat tctaataatc gtcctgattg
     1021 tcatgatcct tctcatccta ataattttac actagtttgt gatggtctac ccatgcaatt
     1081 taatctacgt aactttagtg tacatcagca accatattcg ccttgtagga aactaaaagg
     1141 tttgactttg gaactgattg tagtaaataa gactttggat tcgtctcttc aaaatcccga
     1201 gaatagaaat ggcggcgata ctgtatggtg tgcgaatgag aacattcaac aaaatcaaca
     1261 tacaggtagc aataaactac cgatcatagt ccctcttaag agaggattat ggtatgaatt
     1321 ccttttgcat gtttattgga gattgtgtgg gcagtatgat attgcacatg tacaaaatgg
     1381 aacatttgat aaatgtttga ataatcaaaa tggttatcag gaaatgtggg ttaggcctgt
     1441 ttcgaatact gcaagtactc agaatcctcc tacttatgtt ctaaatttaa cacactataa
     1501 cttggatgaa acatataatc cagccgccga aaataatagt aagcctggcc aagtatacat
     1561 gacacaaggt atttacgatt gcggtcctct agataacaag acaaattgta tacatgaaga
     1621 ccatgtaaca atatatcatg aagaaatgga ggttcttaaa tgtacagatc aatttccaca
     1681 aaaacttaag caaatgatat gtaattttgt gaaatagcct tttagttctt ggtagttttg
     1741 tgccaaatga agtaacgaaa gtgagagtct ctttgatcgg ccacttgtca cccatcatca
     1801 ttggttctgt tttgattgac ccgtgcgcag ataaattctt aaaaaatagt taccaagata
     1861 cagtgttctt tcccaactag gtcgattcac gatgccgaaa attggctaga ctagagtata
     1921 gattacgact cacgcatagg ttcttaagat tacacaaaaa attgtaattt atggcagccg
     1981 attcttagtt gttatctgtc agagtaggat gactacataa ggcatatcct gatgattttc
     2041 tagtcatatt gataacggtt tgatgtattt gcataatatt ttcaggcata attagatatt
     2101 tgtatgcata attgatatca tttgtttcta gttcaccgta tccttgactg gcatctatcc
     2161 atgccctgtt atggtctcct tttctttcat gatcacttgt tgttatttcc gctgtttgaa
     2221 aagtagccag atagagagct atcaagtcat tttcaaatgc catatgtggc ttctttacga
     2281 gttcgccaat ttcagatata tccatcttta atctttcagt agttgttatg gcaaagcctc
     2341 gtgcaagttc gtattgcttt attattgtct gaaaagaatt gaaggtatgt actgccggca
     2401 aatgaatcag taggacggag ttgcctccac ctccttcacg gccagacgac ttgactaatt
     2461 caactgtgta tttatcatac ctcattgtac atttagagac accattcttt agttttttcc
     2521 atctaaggtt tacaatttca gacatatcag ttaccttgaa gcaaaacgca attgaatgca
     2581 atcttatcgg taaactatga tcgtcataaa tagtagtatt attactattt acactccgtc
     2641 tcagcttctc ttttaagata aataatcctt tttccgtgag cctccaaacg ataccatctc
     2701 cttccttatc tttgctaatc aaccctgtac tacatagcct atctgtgtag tagttcaata
     2761 attgagggga ttttttcaaa tgctctgcaa tctgcgatgg tctataacca ccgtatattt
     2821 ttgaaagaat aggaaaagtg aaagacgagt taaggacgtt ttttacactt ttttcagcga
     2881 tggatttggc ggacttttgc tccttcattt tgcgttgtct attaaaaaac ttgtcctagc
     2941 gaatattgtc tggactaggc atttactaca agcttttgtt agatcatcat ggatgcattt
     3001 ggtcatgcag tcaatacttt tgcatttaac tatgcaggat atatcacgat aatgagtagt
     3061 actgcgcagc aatacactat aaggcaaaat aaaattgaaa gtaaaagaat ctttgatcaa
     3121 aagatatttt ttaagacagc tgctatttct gttataagag gtcattgaaa taatattctg
     3181 agtctgcgat gtcctagcct tctgcaatga ttcattatca attttcatct tttgctcagt
     3241 tactaatgct tgattccgtt gtccttccta ctttagcaga aattactttt ttggtcttta
     3301 cattaacctc aacattgaac caaacagtac tttttcctag ctgatactct ttagccgtat
     3361 actgttgtac atctagataa ggcaatgaaa attcaagatt caaattatca ggcacatctt
     3421 catttgaaac ttctggtgaa tacgaatgtt tagttgtcgt atccgctgtt gacaaagtag
     3481 ttggcttgcg aagagcttcc tttagctcgt gatcctctac gttcaactcc ttgcaactcg
     3541 aacactcttc taaatctgat tcgatttgtt tctctgatct gttttgttgg gatttctcga
     3601 cggtaatctt gctatcatcg acaatggatg tctctggcga aacttcagag atgggtcgtc
     3661 cttgagtatc tattacaatc ttcttttttg cttctggtgc tgcagaagat gcagcagaat
     3721 tgcgcttcga ttggcgtaat ctccctgctt tttgcttttc tggattcttg gcttcatcgg
     3781 gtaatgcatc aagtattgta cgtttagacc atattccaac acaatctttc tctatcctat
     3841 cccttgcgtc tgcaggagga atctcctgat tctcgttgcg aagtgcttgg tacatcttag
     3901 gaacgtactc cctagctgtc gattgaaaag cggccgcttt tacttttgcc tctttaacta
     3961 aatcatcata ctcactcatg acgatgacct ctacttttca ttcccgaatg tatcgattac
     4021 gatgggcact aggtcttcgt cggaagattc agtcatgaat tttgatttac attcttcaca
     4081 aagaaataac gcgatggtcc cagcagttcc tactcatacc tccagcttaa tcgtggcctt
     4141 tgaaaaacat cctaccgctt cacaagtaat actattaata gctaaccccg tttcgctcat
     4201 gttcgtgata agctcatcgt aggtctgttc tttttgtacg agctctcttg tcttactaat
     4261 ctgagttgta tttctgtagt ccatatataa tagaacgcaa gacgtatatg tatagatatt
     4321 ttatatacaa ccctatacat cctctatacc atgccgaaaa tactagttga cacgaatgaa
     4381 ttaaaaagtg tcataaatag tgttagcagt atattaaaga gcggagttga gaatgtaggt
     4441 gaccacagac aacaacaaca cagcgtcaat tatgagttaa atgaaaaaga aaaattaatt
     4501 ctaaattata tagaaaagaa tgaaggtact acgcaagaag gattaataaa aaacttggga
     4561 aaatacacaa gggtccccat ttccaaaaac atacacactc tagcaaaaga gggattgatc
     4621 ctaataagaa acgatgaaca caatagtcaa actcaccgtc tatttatcaa taaagaaaat
     4681 gctctagtta cgctaattca ggaccttgat ttttttaaac aagcttattt caagcttata
     4741 gataagacaa aaggaaaata catcgaacta aatcattctg cgggtgtagt aattaaacaa
     4801 ggtaaggatg caacagatct ttttaacagg gtttgcgact tacatgagtc tatgctactg
     4861 ccttacagct tcataattat gaaatataat ttttcggatt tattctccat gcctgaacac
     4921 tgggccaata aagattttat tcaacggaaa tttgcaataa tttctcaaac gatacaagaa
     4981 atacatctaa aaatatatga acttaagccg cgtctaccat cacaatacac tgaaagctgg
     5041 ttaatgcaga cgttatggaa cagccaatat ggactaacgc ccgaaaactt atctcacatg
     5101 ttagaaatct ttagagaata tgacttgagt aactacgctg aaacggtaat cgattcgctt
     5161 tggaaaatta gctattctat cctacccact atgcaccgga cgtattttga actcagagga
     5221 aaagaagaac tcaaagattg gaggaagatt gttaatgact atgagcttga ggaaatatat
     5281 tacaacgatc tgaaggaaga agaaaaccct gatactgagg aagaatatat ctcttaagat
     5341 gcgttaagtt ctctaaataa caaaatcgct atggatgcta tcttgtaatt gttttcgttg
     5401 ttaatgcctc tggtttaatg tgggctagct gaggattctg ttgtatcatt gacatgattt
     5461 gagtaaactg ttggttcatt tcttcacgca ttgctttcat gtcttgttca tattttcctt
     5521 gcaatctttg aatctcagat tctttctctt tttggtcttc cagagtctca ctatacgcgt
     5581 catacgttaa taccattcta cacttagcac agaacttact atcatattta ttggattctg
     5641 agcagtgagg acatgatttt ggttttaata catctgatac tttctgatcc ttagtaacta
     5701 ttccatatgc ttctaaaagg ccattacttg attcattacc gaaatagtga aaatatttct
     5761 cagccattgc agagccttgt gtccaaccag catattgatt cattaagcca gggattttga
     5821 gtgacttttc cgttaatcca gtatgtcttc taatgtacgg attccatggt ttctttagta
     5881 actccttaat cttctgtttg tcttctggag gtatattcgg attttcaagc agttttggaa
     5941 agtatttctc tttgtaatcg tcataaatca tatagagttg atttacccct attgactttc
     6001 ctatactcct gcgaagcccg cagataaaag gggcatttgg attacctggc tgtgggtgct
     6061 cgtggtctag ataatctttg acataaggga tagagtcaaa cagtggtata gatctagtcc
     6121 ctgtttttcc attaaccaat acctctgcat actgttttgt tttgtcagga gttagtttga
     6181 atacaatatc tcttaccttc agctttagga tctcatgagg tctacaactg ctatctctag
     6241 atacagtatg atagcatttt atcctctttg aagggcaata ctttaggaac agaagatcgt
     6301 cagctatggt ccataaatct gtaggtttgt aaatgcttac ttctcttctc tttaagagtg
     6361 gaatattttc tacaactggc ggtttaggtc tagtgttgga tgacatatca gggttgtata
     6421 accatctgaa gaattttgtt aatatggtat tataataatt gtaagtgcca atccacttat
     6481 gcataggatc atccttctca ggttttctaa cacaatttaa atacgataaa aagtcttttc
     6541 tagttacaga tttgaatggt ctatttttgc aaaaagcaga gaatatgctt gttatcttta
     6601 tgatgctctt cctataatta tctgacaggt tagtttcaga tttcatagag gcaagatagt
     6661 cggctatggt caaagcgttt tgtttatttg acagtttctt aatgcttctg taatattctg
     6721 gggctaatcc ttctgcggca gtttgtattt tcatgtcaat ggtttgttct ttggccgctg
     6781 tttttctgtg ctggatagct ccctgcatat ctttctctgt ttacacgaga aatataaacc
//
LOCUS       DAOR01000038            2529 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_46001, whole genome shotgun sequence.
ACCESSION   DAOR01000038 DAOR01000000
VERSION     DAOR01000038.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_46001"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    790..1005
                     /estimated_length=216
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          815 a          390 c          418 g          690 t
ORIGIN      
        1 agaaattgaa tttttgatta cactcgacta tttctttttg ccactaggct ttaagttttg
       61 caattcactc tctacgagtt ggacatgcac tttcttactt tttgcagcaa tgttatcggc
      121 agagatcaac ggaatcaaag gtttgatcaa catcgattta ttacgcgtta actttcagcg
      181 aaacggaaaa acatttcaat tgtatttgcg taaggcttat tatcatcaag agtaaaatgg
      241 ctgttctcga tctgccatct ctaggtttac caatcgttct acatatctga cacagtccgt
      301 aacgcaatct atatggcgag attgacgata aaccagacga taagttaaac gccagattag
      361 gctaattcgg aatagccgtg tcacaccata agtaaataga taatttccag acaaaccgaa
      421 actccacaca tttacagatt cttgaagtgc ctatttcata acccatagag gatttgtctt
      481 tttcatcacg gatagccaac ccatattcgg tggctgaata tccatttgcc atcgtatggc
      541 tattatttca cctctatgat gcagttctac ggtaaaaaca tgaattagca catcttttac
      601 aagagatgtt cttcttacgc catcgttatt tactctaaaa acaggcttta gtaaatcttc
      661 tttctgaata tcggttttcg taacctcttg attgtaatcc attaaagcat tccacgtctg
      721 atacttatga tatggaaatg gtctatctcg agaagatgga ctccagcaaa aactttattc
      781 attcgttcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaaa atcaggatag
     1021 cccactatga aatgcttatc tctgaaacaa ccatatgcag aacttttgat atcaggaaaa
     1081 aagactatcg aattgagaaa ctggaacacc aaatttagag gaaaattctt gatccatact
     1141 tcaaaaaaag tagataagga aagatcggag tccttaggta tcaaccatga gaatcttata
     1201 agaggagcaa taataggaac tgcaaatttg tatgacgtca aacaatataa gagtaaagca
     1261 gaacttgaga aagataaaaa caagcattat gcagatatca aaaaatttgg tgcctacaag
     1321 tatggattcc tgataaagaa tgcgcataga tttcgaacct ccgtcgcgta tccgggcaag
     1381 ttgaaattct tcgaaattga gcatccaatt agcatttgag taaaataatc tctaattgat
     1441 ttattcctac tgtcaataat ttcacaagtt aagttttctc tatttttgac tttgcaagca
     1501 aattacacag taatgagatt gactattggt aaaaacaata atgaaagata tcggcatgat
     1561 ccctgtcata aacgtctaag tggtagactt atgtaataaa tgacacaaac cttatactct
     1621 acaaatctac tctatgatct gattgtgagt agtataaaca agaaaaaggc acccgaacag
     1681 gtcaggttat ttatctgaaa ttggagaata tattggacat atagaagaga gggagtcagt
     1741 agaacgaaaa aatgcaaaga tatatcgtat aattaaacag agatgtcata aattctataa
     1801 ggtgatctgc actactatag aaaataacaa ggacaaagaa ggaattattc ctatttatga
     1861 acaaatcaaa aaatccaggc ttccatggaa tcgcatctca gaaattctcg aattaatcga
     1921 agagaaatac cgaaatgggt ttatcgtcac ggatacatgt cttattgcaa aatcaaaagc
     1981 aaagatgtta aaatcattat tgaatgaaga accgctgcgt gacgaggcgt gtttatcact
     2041 tattaaaagt ggtgttccta agtcatgtat tgttcttgat ttagaaaatt ttttgaataa
     2101 attgggcttc cgtactcggt ctgaggattc aaaggtggag ttagaaccca atctgaaaca
     2161 taatattttt agagttgtca aattgaaaaa acggtttggg ttgaaacatc ctttgatttt
     2221 tcaataacta gaatacatgg gaacaagtca tcatttgacc gcagtcgttt catattggga
     2281 atgtaataaa aaaacgctta agaatttgtt tcatttaatg gtaaataacc atgatgttag
     2341 acaaacataa tattaaatac atcaagcata acagggcgag attaaaggct ggtaagagag
     2401 aagaaagcac gaaaagactt ctacagttct ttggtgcctt gaaggaaaag taaaagggat
     2461 gagtgggttt gtaaagcctg aaagattccc aagaatcaat tgtccttaat tgctgcgtcc
     2521 attggttta
//
LOCUS       DAOR01000039           27786 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_33705, whole genome shotgun sequence.
ACCESSION   DAOR01000039 DAOR01000000
VERSION     DAOR01000039.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 27786)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27786)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27786
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_33705"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         7962 a         5938 c         5572 g         8314 t
ORIGIN      
        1 cttaatttgg aagacgagaa acttgcacca gaagtattgg ataagttagt agtaaccatg
       61 agtgactttg acaactcgat caaggatata atgccgtcag ctttgcgaga agtctactta
      121 gaatcaccag atatcccatg gacagcgata ggcggtctag aagaagtcaa gcgcgaattg
      181 caggaagcag tagaatggcc tttgagatat ccaaatttgt acaaggaatt gagtcattct
      241 attcctaaag gaattctact acacgggcct tcaggtacag gtaagactat gcttgcaaaa
      301 gcagtagcta cagagtctga agccaacttt attagcgtaa aaggacccga attgatttcc
      361 aagtgggttg gggaatctga gagaggtatc agagaaatat tcagaagagc gagacaagcc
      421 tctccttgtg tgatattctt tgacgaaatt gattctattg ctcctactag aggaggaggg
      481 atggaaggag gtgtacattc aagtactgaa agaatggtat ctcagttgct tactgagatg
      541 gacggtatac agaaggatat tcatggagtc gtagtccttg ccgcgactaa cagagttgac
      601 atgattgata cggccttatt aaggccagga cgatttgaca agatcgtata tgtgccaaaa
      661 cctgacgtaa agacaaggca gaaaattctt gaaatacata caaaaggtaa acctttaggt
      721 cgagatatag atttaaagcg aatagcagag gtgactgagg gatttagcgg cgcagatgtt
      781 tcagcggttg ctaatactgc agtctctctg gtactgcatg aataccttgc aaagtactct
      841 acgccggaag aagcagcaaa acattcgtca gaagcacttg tttcaatgca gcactttgag
      901 gaatcagtca agaagataag gattcaaaga gaaatgaagc ctcaagagag aaccactcta
      961 tcacaatatg gataatatta taatatccgt ataggtagcg tacctaactt gtgttttttc
     1021 cttctcaaac ttcaccatgt atttacaata ttgtttaata gaatattgat ctggagcttt
     1081 atattgcatt agatgagatg acattgatgt catggccaca ataagcgaac accaagaaag
     1141 atatggttgg tggctaaatg aattggattc actgggtatc agtacgaaga gaataagcga
     1201 gcttactgat ttgaaggaaa tccgcaacct gactgagaat ataaaacaag ccttttatca
     1261 ggactatgag accgaatgat acgatataat atgaaatgaa tgctaatgta aacttctacg
     1321 gtttctagtt cgaaaattcg gaaacgatga gattattctg tacgaggcta ttcgcacgaa
     1381 tatttctaag acagaagcat ctattagtgt tgtagcaaag gcacgtggtc gtagagagaa
     1441 aaataaaagg aaggtgatac taccaggaaa tgatatcgta atggaccggg tgggattcga
     1501 acccacgacc acctacgttt cctctgccat ggcatgcaag gcaggtattc taccggacta
     1561 aaccaccggc cctcggcgtt atgcttcgta gatgatattt taatctttta ttttccattg
     1621 ccctatattg atgggcttgg gttgtcactg tagtatagag cgagataaat ccaagtaacg
     1681 cccttaaaat atttattgct actacgtttc gcagaatcga tttgtacgaa gtttgagttt
     1741 cagctgaaat gagcaagaga aaagaatcct actctaatgc taaggtcgcc atgtgcatac
     1801 ggaaaatcgt atagtaagaa cacatgacca accccacagc ctcctagaat agggatacca
     1861 tttggccgcc gctataagag tgaccgaaag aatgaattcc cgagccctta cggcttgcag
     1921 atatcaagtc tcaagcgtta acaaattcgg tactttaaca gtaactcact aacaacaaaa
     1981 ttctttccac aagaaataag ttggagtatt tcggtactac ctatgttgtc attcttcgga
     2041 ggaaatccta gcggaaatgt cgataaaagt aagtacgagc atcttacgat gagtataaga
     2101 caaacagtcc tcgaaagggc acatcgtcgg tgtcaagagt gttctgtgaa gtttagtcca
     2161 agcgtccatc ctaattttga gcatataaac ggatctttga aagacaatcg accaattaat
     2221 ctacgtgcct tatgtccaga atgtttcaaa tcggtagcaa agaaagaaaa tcgcaaggga
     2281 atccttggga atattcgcta cacaattgga ggcatgctaa agtagggaaa tacagccaga
     2341 cgcacactat agactcataa agctattgaa gggattcaga agcaagccac tagatttaca
     2401 agaacacttg tctatggaca ccatccgtta ttacttctat tgcatttcct tttccttctc
     2461 ttggcgctcg aatgtaaggc agagtttcac actttattaa cggattttcc agtggtcaga
     2521 ggtagaattc ttcttttcat attccgcaat ttgggactcc atttttaagg taatgtctat
     2581 atcatctaaa ccctcgagca atttcttttt tcttgactca tttatttgaa aatgtaggat
     2641 tttatgagca actaacacct cttgtacggc caaatctatc ttgatctctt catcataggc
     2701 gtctctaatc aaatactcta catctgaggc gttcaatttg attggcaaaa ttccattttt
     2761 aaaacaattg ctataaaaga tgtcagcaaa ggatggtgct attattgcct tgaatccgta
     2821 atcttcgagc gcccacactg catgttcacg agaactgcca ctgccaaagt tatcacgtgt
     2881 caccaatatc tctcgtcctt gatatttcgg atcatttaat acaaaattgg gtttcagctc
     2941 accattctta tcaaagcgcc aatcataaaa taagtatttg ccatagccgg tcctttgaac
     3001 caatttgaga aactgcttag ggattatttg atccgtatct atgttggcca tatcaagcgg
     3061 tgtagctttg ctttttatga ttctaacagg ctccattctg gtgtccccca tgcgtaattt
     3121 tatcctgagc ttacctataa ccaatctcta atgtcgacaa aatgaccttc gatcgcagcg
     3181 gctgctgcca taactggact gacaaggtgt gttctaccgc ctgttccttg tcgtccttca
     3241 aagttacgat tcgacgtact ggcacatctt tcgctaggag caagaatatc ggggttcatt
     3301 ccaaggcaca tactgcaacc cgactctcgc cattcgaatc cagcagccat aaagatttta
     3361 tccagtccca gctgttcagc ttccctcttt actttttgtg aaccaggcac taccatcgcc
     3421 ctaacgttcg gtgatacttt tcttcccttt gcaatggtcg aagcttctaa taagtctcct
     3481 agcctgctgt tagtacacga cccgatgaat acacgagcaa tcggaatctc tactatagga
     3541 gtccccggtt caagcgccat ataatccaga gcgcggaacg cagcttttct ctcattttca
     3601 cttccttttg caaaatcggc tgggaaggga atagtttcag tgacatctac taccatcgat
     3661 gggttagttc cccaactgac ctgtggtgca agatgatcta cattcaaaat aaaagactcg
     3721 tcgaaccgtg cattaggatc tgtcgtcaaa ttttcttccc agaattccat cattttttcg
     3781 aactgttctc cttgaggggc atatcttcgt cccctgatat actcgaaagt agtcctatca
     3841 ggggctatca atcctgctct tgcgccagcc tcaacagaca tattgcaaat agtcattctc
     3901 tcttccatag aaaatttgga tatagtttcg ccccggtatt ctatcactgt acttgttgct
     3961 ccagaagtac ctatttggcg gataagggaa agtatcacgt ccttggcagt tactgcatgt
     4021 ggatttctag gactaccgac gatatttatt gcaaaagttt ttggtttatc catccataag
     4081 cactgagtgg ctaatacatg ctcgacctca cttgtcccta taccgaatgc aatagtgcca
     4141 aaggcaccgt gtgttgaagt gtggctatcg ccgcaaacga tcgttgttcc agggagagtt
     4201 attcctagct ctggagcaat aatatgtaca atgccttgat ctgggctatt gacatcaaag
     4261 agagttactc cgaagtcttt acagttttgt tccaatcttt ggatctgtag agatgatgta
     4321 ggatcgacaa tcggaagtga cctattgtct gtcggtacat tatgatccat cgttgcaaat
     4381 gtcaggtccg gtctcctgac agtcctgcct gttgttcgga gaccggcaaa tgcttgaggg
     4441 gacgtaactt cgtggatcaa gtgtctgtca atatataaca gcgacggtcc ttgttcgctt
     4501 tcatttccgg gtcctacaac atgactgtcc cagatcttct caaatagcgt ttcagacatg
     4561 agcttatctt taattaactg agctgaaata tattcattgg cggttatcgc aacttcagat
     4621 gccaatcacc agtacaatat aatctgttca tattatatgc gtcatgtctc atccccgatg
     4681 gaatcatttt attatgagca atatttttga gaatacttaa atctggtaac gattctgata
     4741 ctcgtaatat gcgttcgcag gataatcgat acgaagaata tattgcagag cacatcagaa
     4801 aaggaacgac tacttgtgcc ttgacttgca aggacggagt agtgttagct gctgacacaa
     4861 gggcaagcgc cggtctattt attgcagata gacatgtaat gaaaattcaa agagttgata
     4921 atcacctggg tatgactatt gcagggggag tagcagacgc ccaaaatctt gttgatatta
     4981 tgcgttacaa tgcaaatatc tacaggattt cgaacaaaga accaatacct gtcaagtctg
     5041 ccgctagact ctgttctaac gtattattca atcagcgata ctttccttac tacgtgcaga
     5101 taatcctcgc tggtttcgac agtcagggag aaggtcagat ttacaatatt gacctcttcg
     5161 gttcaatgac aagcgataag ttcatcgcta caggtagcgg ctcaccagtg gcgtatggtt
     5221 accttgaagc ggaattcaag gcagatttag gtgtgaatga cgcgtacaaa gtagccgtcc
     5281 agtcaattgc agcggcgatt agaaggaatg cgggtaccgg tgacggcata aatgtggtta
     5341 ttatagatag aaacggatat cgtgagctgt ccaaggaaac taaagcggct gtcggggcgg
     5401 ttttttagga cattcctcgc gggtagacga tgatcggtgt aatcctgatc tgtaaagtag
     5461 taaccttaat ttagttttca aaccgaatta acagtgtgtg attatcgtta gacttttaac
     5521 ttatttccta tttaaggaag gtgatacttt atcaaaacat atctcctgtt agacatttct
     5581 ccgaacaagg taagttaaaa aaatcagaag caatgtgacc aattttttaa taatcgtgca
     5641 atctgggtac accctgggta gtagaggata gatgtacgtg ataagtaact aaaaaacaaa
     5701 agcgtaccgg gggttataat tgttagctcg atctctgttg ctccaccctg ttttgactcc
     5761 tctactactt atctttccac gttcgaacac tatgaatttt acgggctagt gatttcgtag
     5821 ttactatccg gattagaaca ttttgctaag agacaagatc tcttttaact ctgtacaatt
     5881 tcttgttctt tccatcaatt atcttcaaat ttctttcgaa tgaaatgaat agttctgtta
     5941 cacgcgcagc cacaaataag cagctacgtt tgtgcatgat ttcctcaggc aaaatattat
     6001 gctgcattca ctcttggaca gacatttcaa atccagtata atctctgaca ggtttaggag
     6061 agttccgatg aaccatctat cgaaaggtct ttgctccagg attcggaatg aattcgcaag
     6121 agattgtgag gaaaaataac tctatcaacc accgtaccaa ttccgcgaaa tccctctcat
     6181 ctctaattcc taatgctgac ctcaaatagc ttgctttcgt gagtctttct aagaagatag
     6241 gagaaatctc tactatttcc ttggaatgtt atacctttct aggatctatg gaagtggcac
     6301 acatcgcatc ctcaggaaaa catccgcgag tgattgtcga actatcgact ttcactttta
     6361 tacggaatac aaatagtggc acctgaacaa agaaacaaga acggtcatga gtatttgttc
     6421 tttctatttc tggtatcgaa gctcccacaa tttgatagag gaaaatctat tctctggagg
     6481 attctttaac actgcgagtg gattaataga tgagacatat tattcagcgt caaaataaac
     6541 acggttacca aaaaagtaaa cgagtttatt ctttgattcc ttgcatgtgt actctaagtt
     6601 ccagtttttt tgtgcttgaa tcggcggtag tctcaagata cgaagaggca gctttgaatt
     6661 ccggagtttg agttcggcca acgacattgt gatgtctttg ctgaaggaaa aaccggcata
     6721 ctgtttatat cgccagtcga tttctacgac ataaaggcgc ttatcgattt tacctcagtg
     6781 ctggaaatta ttggtccatg agagaatcgg atttaatacc catgatttta agtgtgagaa
     6841 atcagtatat attcattagt agcctacgta tagcctgctt gaattagtgt gtacacatat
     6901 tagctgttta gttattcaat tatgacattg cagtttaacg atattttatt acttttattt
     6961 aggatgactg gctttagcag taactgggtt agagaacgca agccaaatgc acctgagcga
     7021 ataagagaaa gcgtgagagt acagaaacca ctcaaaccaa cgattgattc aacaaagaac
     7081 aaagttcatc tccaaaatca gaagctagat acaaccctag agaaattaaa aggaaaagaa
     7141 aaatcccttt tcaatcaaat catcttaaat gttcagaaac atgatatgcc tcagggaaag
     7201 atgttatcga atgaactagc acaggttaag aagacgataa aaacggtctc gcaatttaaa
     7261 atggctctcg aacaggtaca tttcagattg gaatcgacaa gtgacatggg agatgtaatg
     7321 agcgccgtag gaccagcgat gggcgcattg aaaagggtaa aatcgggaat gtcggacgta
     7381 atgcctgaga tagacacaga attgggagaa atcaatggag tctttaatga tatcgtgatg
     7441 aacgctggag agattggtac tacatcattt gcgtttaatg tcagtggcga agagactgac
     7501 aaaatattgg cagaggcaaa tgcagttgcg gagcaaagaa ccagtgataa cttcccagat
     7561 attccgatgg gaacatttgg cagggcctcc agatcgtctg taaatgaaca ttcacaataa
     7621 aaataacgtc aaagaatcgc taattttttc gctgggattt tttttacagc tcgtcctgcc
     7681 tagcgtggtg acatggacta aggagaaaac tgttattata gcctcgaaca tatagcgtaa
     7741 atttggttat gggcaaaaaa aagaattccg gggttataat aatctctaca tttcccactg
     7801 aagaatcctt aagagagata gctacagatg tgattgttaa taaaaaaata tgcgcatgtg
     7861 ttaattatac aaagatccga tccttatact tttggcagaa taaacttgag gatcatgcag
     7921 aattcattac actgttcaag acaacagatc aacgttcaga agaattaaaa gcagaaatag
     7981 agaaaagaca cccctacgaa attcctgaag tagtagaatt gaagatgaat gacgtctctg
     8041 aatcttacgt ggcttggatg gcactatcaa ctaatagtgt tatttagaca aataacaatg
     8101 gcatgacaca ctggagataa aacgaatgga ttgcacttct tatttatctc atggaatacc
     8161 taagcaaggc tattatacct cctaaagacg aaaccctgag accgatatca gtagaggaat
     8221 caacggcata aattttccct ccgtaacttt caatcgaatt taatactttc agaactgcgt
     8281 cttcgtcgct ggtcttgaag atagcatctg aaaataccac acattcgata ttctttatcg
     8341 acgcggcgta agttacctct tccattccca ttgcatactt caattcacca ttatgaacga
     8401 gtttcaatac ctcgtctaga attgaagata ctgctgcgag tttgcttgaa ctcattgcct
     8461 ctttgagtga agatgagcga agaaagacat agattccatc ctctccagcc acatccactc
     8521 catcaactat tgtcaatttt tctttttcaa accgatattt ctctgtacct aaaaagttat
     8581 gaagtttacg tttagcttca ccgggaccga atattattgc tctgcaggca tcattaccac
     8641 cgtcgttgtt cataatactt tggatcgtct tagcaacctc ttcaaaaaag ctctcgatat
     8701 taggattgct tttcctagac gactggtaac gttttccact ttgcccagaa tatatatttg
     8761 gaattagctt tatatgagta ccggaaacct ttgcgactga tgcctcttgt gtatctatcg
     8821 cgaccaagat aaaatcttct gtgtttcccg atctggttag aatagttaat tctgcattat
     8881 gccactttct ctctttttca atcattattg tatcatcgat ccgcaccgtc atcgaatgat
     8941 gtgaccctat ggaaacaagc tcattgtcag tatttgttat aatgcctgta atccgtaatc
     9001 tatctacggc atcataaaga gcagtatttt caacttttag agagaccctt accttaacac
     9061 gctctcctct ttccggccgc ccatattgct tgaggtgctt gattacccgc gttgtatctc
     9121 ccacgacaaa gtccccattt tctattgttc ttcttaaagt aaaaaggtca tctgcatctc
     9181 tgggtatgac aatatatgct ctgcctgaaa accctttggc ttctctcacg atcatttgat
     9241 gaggcttcga ttatcttgtc tggacagaaa cggattgtcg cattctatgc tactttctta
     9301 ttggttatcg aagaagaacc ttcttcctta gtcccggccg attttaaata attgtcgacc
     9361 cattcctgtg tcaatacccc agactcgact aaattcacta acgcattcgc cgacgcatct
     9421 ccgtcaacct tgcctgacct tcttctagtc tgtcgccatt ttagttgtgt atttccgctc
     9481 ttcgaggaat aaattatgcc cagtacttcc tttgcgttaa caaacgtcat ctcgcgcact
     9541 tttttcaatg tcaccttatc tgctgcctct atatagatcg cgcccggtcc atcttcacgt
     9601 tctggaaaca cctcatagtc gcgcaaataa ctttcaggga taaaggatcg acaagtgcta
     9661 acaatgataa actgtcgaaa actttcgagc gaagcagtag ttacgattcg gaccattaat
     9721 gtataaaaac atttagatgc ttatcaaggt tgtgaaataa acggaataat ctctttcacg
     9781 aacaccaata atttctgaat agtttctttg ggtcttatat atgattattt cggcacctca
     9841 gtgaagtaaa gacatgccag gaaaagaggc agcagttgta caagtacggc aacaactaag
     9901 ccaaggctgg acaaactcct aactcaagat ctacttcatc tactaactca ggagggctag
     9961 tttcgtcatc gtttgtccgt ccgggacttt ccattatcac acataaaaac cgaggctatc
    10021 tacttctctt ttcgtagata gcttgtgtct cattaatttg taagaccaac cccaacatga
    10081 acattttcac tacactcatc ttaacttttg gaccagatta aattactatt atactagact
    10141 gtttactaga aataatgggc gcgctaaaac aacaaaggtg tgagaatcac tttttaccta
    10201 cttttttccc cggccaaacc tgtctcttgt ctgctggtag catcagccca taaataatga
    10261 aagtaatcct ttgcaaaacc tgcaagacct atatgttctg cccatatagc gattggttca
    10321 aatcttccaa tatcgcttct tccttcaccc aacaatatca tcacctgcct gctgtcacct
    10381 attactcctc caccaaacat attgtttttt aatctaacat ctgcaattct agatatcgcc
    10441 cgaacagtat cagggttggt ttgctcagat acaagaacgg ttatccttac tcctttttcg
    10501 tgcaggactc tcagcatcgg ctgtaccggt cttgcgatct cttccacgac ttgtggtaaa
    10561 gctattagca gttcgtgctg gcaggtctga attatttcac ttacttttgc cgctatattg
    10621 taaattcccc taacaaccca gatctccggt ctttctcgta cgccgctttt ctcgtacaca
    10681 ggcattaact cgcttacaat caagctttcg ttgtccttaa tgccgttttc gatacgcatt
    10741 ctcatggcct caagtgcagt acggggtgat ctaggaaaga atttctgcgg tctagaactg
    10801 tcagattctg accatccttt ctcttctagg ccgttaagaa cttcataaat cttcgagtaa
    10861 ggtacacccg acttcttgct taggtcagac gctgttgatg tgccggcttc agcaagagca
    10921 atatatgcct taatttcgta acccgttaag ccaaggctct ccatcgcttt ccttgactta
    10981 tcgcttatat tcatctaaat atttgaactt gtcttagagc actaatatag gtatgttcta
    11041 aatatacgag cgacagcggg gagagaaagt ttttgttagc gctgggatta cttagagaat
    11101 atgtgtactt actatatgtg aattctaacc agcacaaatt ggacccaaac tagaagtggg
    11161 gttgttcttc actatgacta gattgtcgag gtttacaagc tgatgtaagt tctactagct
    11221 tgcgttgctt gtgtgctttg tatatttgac gtccccaatg ccatatatca tatcgaaaaa
    11281 taacttgccc tactaaatcc attcatgatg cctatcgtct taataatggg cgacggcaga
    11341 tacacaccta aggctggcca tcggtccata cttcaagtgg ttccgttccg cttcccacct
    11401 tcggctcttt atcgagctgg ggtttccagg gtgcaatacg agaacctcct tctccatcaa
    11461 accttaatct gcaggaagta actaatcacc ttaggagttg tcatgttata cttatataga
    11521 taaagcctac agcaacaagc cgagtagtag gagtagaaag atagtaaaat ggaacttatt
    11581 caaatttcta atacatccag taagagttct ggttcaagac attcgataag gtacactcag
    11641 agtatctgcc cagattgcaa catgatccta gatgcagaag ttttcgaaag ggaaggcaag
    11701 gtcttcatga caaagacctg cccatctcat ggggaatgtg aggagctgta ctttggatcg
    11761 tatgagatgt acaagaaatt cagcacatac tggatggatg ggaagggtgc taaggctccc
    11821 aacgttatgg tagataaatg tgcatgtcca accaattgcg gtttatgcac aaatcacctc
    11881 tcgcattctg gattggctaa tatgattgtt acaaacagat gcgaccttac atgttggtat
    11941 tgcttctttt acgtaaagaa gggcctggaa ggagcttatc tttatgaacc aaactacgaa
    12001 caagttagag caatgatgaa gacgttgaaa gcggaaagac ccatcgcagg taactctatc
    12061 cagataaccg gcggcgagcc aatgctcaga gatgacataa ctgagattat cagaataatg
    12121 aaggaagaag gcgtcgacca tgttcagatg aacaccaatg gtattaaact agctctgtcg
    12181 ccagaaacta tgagacaagt aagaacagcg ggtgtgagca atctttatct ttcttttgat
    12241 ggtgttactc ctagaactaa tcccaaaaac cattgggagg taccgtatac attggaatct
    12301 gcacgtaagt gtggcatgac agtagtattt gttcccaccg taatcaagtc aataaatgac
    12361 catgagctcg gtggaataat cagatacgca caaaacaaca tggatgtagt tcatgcagtc
    12421 aatttccaac ctgtctcgtt gacaggcaga atgacgaaaa aagagcgcga gaaatacaga
    12481 attacaattc ctgattgcat tgaacgaatt gaagaacaga cccaaggcga catcccaact
    12541 gatgcatggt tcccggttcc ctcgtgcatg cctttaacga atgtaattga agctttcagc
    12601 aagaaaccaa aatacgagct ttcaattcac tttgcgtgcg gtgcaggaac ttatctattt
    12661 gaggacgcgt tgactaagaa gttaatacct cttaccgcat ttgtcgatct taaaggttta
    12721 ctagaatact ttgaagaaaa agcagatgaa attagatcag gttccaatag atactggacg
    12781 acgctacaag tagtacgcaa gttgaaccaa ttcgttgata gaagcaagca accagagggt
    12841 ctcgatcttg caaagatgtt ttcaagtata ttactaaagc gaagctttga ttcagtcggt
    12901 tcatggcacg gaagatcgct attcttagga atgatgcact tccaagataa gtacaacgaa
    12961 gatcttgaaa gattgcaaag gtgtgatatt cactacctga ctccagatct aagaattatt
    13021 ccgttctgtg ccttcaatgt tatacctgag tggtatagag atcgaattca aaagaaattt
    13081 agcactcccg ttgaagagtg ggaaaaggca cacggccaaa ctctcgaagc tggcctctat
    13141 aggggactaa tgagaaaagg taaacccgaa gcccagatgg gttgtgcggt atcagaaatg
    13201 cacaaggcag cagctgagac atcaggtgta gaggatcata agggcgttga gcttcgcaat
    13261 ggcatggctg gcgcgtaaca acaatatgcg ctcgtctgcg taaatcctct ccttttttct
    13321 agaataacgt ttgcaatgtt atcatgttat ccacatggat cgagaccacg tctagtgtga
    13381 atgccgtcat ggtatatctg gcaattgcaa tgcacagtgc taccttcact atttaattgc
    13441 gataaggttt aattgaaagg agtggttgct tcccctatgc ccactatgaa atatatacaa
    13501 ctcgaacctc aagcggacat tgctattatc aagattaata gaccagaggc cctaaatgct
    13561 ctcaacactg atgttattct tgaattatcg cgtacaattg acatcgtcgg cgtagacgac
    13621 ggtataaaag ctgtaattat cacaggtgta ggcgaacgat ctttctgtgc aggcgcagat
    13681 atctcatata tggtcaatat tacaccgatc atggccgaaa aatatgcttc agctgcgcag
    13741 gatgtcctca acaagatcga gaaattagaa aaacccgtta tcgccgcaat taatggattt
    13801 gcgctaggtg ggggttgtga attggctatg gtttgcgaca ttagaatagc ttccagtaat
    13861 gccaagctcg gacaacccga agtcacgata ggtatccctc caggttgggg aggaactcag
    13921 cgattgctaa gaattgtcgg cccgtcaaag gcaaaagagc tgatcttcac aggtaaaacg
    13981 ctatcaggcg aggaggcaga gcagattgga ttagttaaca aagtaatatc tttagggccg
    14041 gatgatgtgt taccaccaga tgctccagat ggggacagtg tcaaagaaaa agaaaggtca
    14101 aatcaagttg ctaggatcct caacaagaag ctaatggaag aatgcctcgc actagcgaga
    14161 caaatcacaa agaatagttt cattgctgta aaaactagca aaatactaat caacaagggg
    14221 atggatgcag atataaatac tggactgcgc ttagaaatct acggctgggc attgtgtttt
    14281 gctcatgaag acagggagaa aatgatgtct gcatttctaa acaaaggaaa gaagtagaag
    14341 ccaataggtg ctgccttact catattaagg gtctactagt actcttgctc ctttgctggc
    14401 gtggcatgct aaggttctgc tttggacgtt cgcttcttta aacccttcac tcatagaatc
    14461 tgctacacgt tttccaaatt tgtcatcttt caaaaatgaa atcatggagg gaccggctcc
    14521 gctgatcgtg acagcaaggg caccagcttt tagtgccttc tttttgactg cgtcatatcc
    14581 gggaatcaaa tcctttctaa gtggctcgac tatcatatcg tcaattcctc tggtgatcat
    14641 ttcaacatct ttcaagagaa aacctgcaac aatagtactg gcattagaaa cattatgaac
    14701 gacactcttc aacgaaacat tcttgggtag gacgcctcgt gcgacttccg tctttttgta
    14761 ggggacttcg attaaaggaa ttgcaattat catgaggaga tcttcagggg gagctaattg
    14821 gataaattct aatcggggac tggttctgac aatcacaaaa ccgccgagta atgaacccga
    14881 aacattgtcg tagtgtttgg cacctgcact gactatttcc ccttgagctg cgtattcgac
    14941 cagtttagtt ttttcgattt taaggtcaaa tagtctgtcg aacgcaactg ccgcggccac
    15001 agccgatgcg gcgctactcc caatccccat ccctgccggg acttttttct caagattgat
    15061 atccaaatta ttctttatac taaaatcctg cgacatcttt ttgactacca cacctgcaca
    15121 attgtcttgc actcttgaag gtacgctttt tacagcctcg cccgagatcc tgatcgttat
    15181 ctgttctgac tgttctgagc gtgtagttat tctaacttta tcttcaaagg cgtcaagacc
    15241 cagaccaaag acatcaaagc ctggtcctaa attggctgta gaggacggtg ctgcggcggt
    15301 gacagacgtt gatcctaatt tgtggatcag tctttaactt cctctccctg actgacaatt
    15361 ctcgctagcg cagccgacaa attgaccatg ccattcaagg tcatacttga aaccgctata
    15421 aggctctgat gaaatccgcc cttactcata ctcctgacca gatctttgct caaaagagaa
    15481 tattctgcat ccttctcatt gtcgagagaa gattctaatg aaagcgcaga agacgaccat
    15541 ttcagaatgt ctttcaattt gtcaacaatg aggtcacgtt tggttagaat attaatctgc
    15601 gcaatcttta atctcaatct aactgacgat gcgagtagtc ctattgaaac aaaattaatc
    15661 ggagatgaaa ctagcatccc atcatatatg aacaaagtaa ccttattgtc ggcttgaaaa
    15721 ttcgatacaa aatatggacc gcttgctctg aaggcgaata gctctatctg tcctggtgta
    15781 tcaatcagga tataatccgg gttcaaattg ttcacttcat cttgaatctc atctaacctt
    15841 gttgcgatca tatcgcttgc cataattaat gaaccatttg gtccaaggtc ataggtttcc
    15901 atcaaagtgt ttatatctat atagtctcgg acgtctacat caggcttata tggcaaactc
    15961 acgacaccag ggtcgaggtt aatactaatc ggaaaggatt tgtgatccac ataccattga
    16021 agcaatctcg acgtaaggag tgattttcca gatcccgcgg taccagtaat aaatattgca
    16081 ttcatatatc agggatgact cattaccgca tctttgtata tttactttct aactccatgg
    16141 aattttttta gtctacctag cccttcttgt caagtcccct cagttctaaa gttattttcc
    16201 atgctacagg ggaattcttc atcagactcg gctgtgataa catgctcact tggattcttc
    16261 tgagtttgat agcagagtgc ctcttaaaag aataaatcat ggatatttta gctaatatct
    16321 agaccgcagt aacagcagtg gctcaagagc ctgcgtcaga gtaatattga gcacgaggtt
    16381 aattttcatt caattaatta ttatatgtct aacttttatc aattaactga ccgcttttga
    16441 actggagact gctcgccata cctgcaagta ttgttccgtt gcttataatg atcttctcta
    16501 ctcatgtttc tcccaccaat atatttgcag taggggtcat tccttttgta atgtcgtcag
    16561 tctcagtaat aggtaaaatc atgcttcagg cgcttcggtt cgactatttt atccgaaaat
    16621 ttataggacc taacgtgagc tccactggaa aaataatatc ggccaggtta gcgggcgaat
    16681 tcataacgca aacaacgcct tcttacgtag gaggagaaat agttagaatc gctttcttaa
    16741 ccaaaaacgg tgtcccggct gggaatgcag catggattac cacaatggag attattgccg
    16801 acgtttttgt cgggactatt cttgccttca ttgcaggtgc cctagcaatt tacaaagggg
    16861 gtgactttat tggcattgcc gtcattctag ttgcggttcc gacattcgtt ttttggcttc
    16921 tgctggtagt gttttctgca aaacgcaccc tccgattgcc ttctttttca ttaaaagtgc
    16981 tacaaaagtt cattgcaaag caaaaagcag agcgacttat taacacgacc aatactgcca
    17041 taatggacct atgtaagatg agtaggcaga attttaattc tgtaagggct gtcaaaattt
    17101 ttgccgtcgg gatcgcaata acatttatgg cgttcttgtt gcagggtgca tcattcctga
    17161 tattggcaaa tgctgtaggc tttaggctgg gtgtatttga ttctctgatg gcaacttccg
    17221 catctactgc gttgtcaaca ttaccaatta caataggagg ttctggactt gcagaactag
    17281 ggatttgggc atatacttct cattttacgg ggatcccatc ttttgcaaat gtagtcaatg
    17341 attcacattt gagcttgatt atagcatgga gaatagcatc atatcatgtg ccgctgctga
    17401 taatgtgggt tgcactcatg aaactaacga tgacgaaaat ttctattaaa tcgctaacaa
    17461 agaaaaataa tttatgggat atcactcata agacctctac tgacgaatct gaaggcacta
    17521 gagagtagaa aacatattcg cttatggttc agcccctata aaaaatttaa cggccgagta
    17581 gttctgatca cagggggatc gtctggaatc gggaaacagg tggcaataga ttttgcaaat
    17641 aacggtacaa gagctgtgat tttagttgca cggtctgtaa gaagcttgcg gggcagcaaa
    17701 ttctcctcgg ctggaaatta cggttccatt ttattttagg gcggctgtgc ggttaaagca
    17761 gaccctcccg taggtagtca atcctattat gggcactgct tctagagaga aactaaagta
    17821 agccctgctg tactgagcat tgttctctag acctcgtcga tatcattccc ggctgcttcc
    17881 gcagacattg gaggagagaa agagacatca gattcgattt ccatgagctg caagtcctcc
    17941 agctcgaaac ggtaaatagc atccatatca agcgaaaatt ttgttttcaa aaattcgacg
    18001 agtctaacac ttaaatcccc tttgaacatc tttatcttaa attcatatac gactcctttc
    18061 ctaaactcgc caatactcat agatttggga acatctaacc cctcgatgat agttctgttg
    18121 tcaggcagag gttcgtaaag ctggatatca attttaaagt cgttctcata agcatcaaga
    18181 atatatcctg atcttttaat aacttgttca tctccaaact gaactgattt tatttcgtgt
    18241 tctgcccatt caggcagctt ttcttcactc ggtttgttag aatcagcttt agctctgccc
    18301 atattagtta tttacgaata aaattaaaaa atctttatct tgatagggtg cagacatact
    18361 ctaattagct cattaattag cacaacaatt tattaactgc tcgatagctt gactcgtatt
    18421 tagtaggcct ttactcttag catattgctc tacgattttt aactgttcaa cgttcaggca
    18481 aataggaata atttttttgg aactacggtt catctttcat tgcaaatggg actagacagg
    18541 gattataatc gttactatgt acataaaccc atatatgcac gcacccatgg tggattgtgt
    18601 cgcttatcca gaaacgcagc tgctaaagcc gcattctatg cacatatcgc atccttcagt
    18661 cattattaat tttgctcgac aagatgggca gaattcgttc tcagttaatc catattgtga
    18721 gcttaaagga tgtgtctgca gagaagcgct cgcaggttcg attttcgaat gttgttggcg
    18781 ttctccgctg agtttgcctt tttcgaggcg ttcgtccgaa tctggtcttt gtatgtcctt
    18841 gaaaattttt tctaatgcct ctagaacata gggttctgaa atattttcac gtatgtagtc
    18901 gattgcatac cgactcggag caacaatgaa cctccgttcc ttagtgtttt caccggtaat
    18961 gtgtagaacc tgctcgtgtc ttgagccatc tctgtataca gtaactccct ttaggccaag
    19021 atcatgtgat agaagatacg cacattttac ctcttctgcg gttacatctc caggcatatt
    19081 gatggtcttg gcaattgcat tagcgatcca cttctgccat atggcttgag ccattaaatg
    19141 gtcagtccag tggatatcga ttgcagtcac gaaaatcttc tgcatccatc cagggatctc
    19201 aggtagccca cgccctgaac cataattgtt tgcaatctta gtaagtattt catcgtcgta
    19261 caaactattt tctcgcaata cggcttcaaa cactctattg gtataaaaga acctgcctac
    19321 cgtgacgcgt ttttcaaaag ccacagcaaa tataggttca acaccattcg aagtgtcggc
    19381 aatcatcgac aaagtacctg taggagcaat agttgaggtc caagagtttc tgatcccgtg
    19441 tttcttgatc ttttcaatga gcgaattcca gtcatatgta tgcatttcct ttggtagttc
    19501 gtagtagcct gcgaccggga tcttcccctt gacgtaatct gtctcttcca acagcggaaa
    19561 cgcacctctt gccttggcta tagcaacact ctcttccatg ctgtagtatg acatagtttc
    19621 tgccagttta tacatgaatt catagccgtc ttctgagttg taaggaattc taagcaagaa
    19681 aaggagatcc gctagaccca ttataccgag accgattcgt ctagtgagct tggtgttcgc
    19741 atcaatctcg tcgacaggat acttgtttat atcaattata ttgtctagaa atcgtgatgc
    19801 gagtctaata gcttgctcat acctttgcca gtcgaattca tatctcccat ctgccttacg
    19861 ctttacaaag ttcgccagat ttattgagcc caaattacag gattcatttg gataaagtga
    19921 ttgctcacca cagggatttg tggcgcgtaa tggtccaccc ttagcattga tacacggatt
    19981 gtatttattt atgttgtcaa agaagataac gcctggttca gcacttttcc atgcgctgaa
    20041 cgagattaaa tccattaagg catgtgcatc aatttgtttt atcggctcac gggtccgggg
    20101 gttgcgtaat gtatacttaa ggtttttgtc ctgagttacg agggatcgcc aaaaatcatc
    20161 ccagaccccg acgcttacgt taaagttttc gaatacgccc ggttttgttt tggctgttac
    20221 aaatttctct acatctgggt gccatacctc caaaataccc atattcgcac ctcgtcgctt
    20281 tccgccttgc tttacaacat cagtgatcgt gtcaataatc ctcatgaatg aggtgggccc
    20341 agatgcaaca ccagaagttg atgcgacaat gtctccttca ggtcttaggt cggaatagtt
    20401 tattccgact cctccccctg atttgaagat cattgcagca tcagtcgatg atttcatgat
    20461 cccttgcatg tcatctggca tatcgagaac aaagcaggct gacagctgac ctagccggcc
    20521 gcctgcattc attaacgttg gtgtgttcgg aagaaagtct cgtgacacca ttagattgta
    20581 atattctatg atcctttctc cgtacgctgc aaatttacct tcagcgatta gtctaagtaa
    20641 ttccttaaaa gtgattttca tatgttcttg cttcgcaaga tcaatataat gcctaatcat
    20701 cgcttcaaaa tgatatttgt tcaaatagta tttgcctatt ttgagcttaa gatcaaagtc
    20761 gtttaatttg gaataatatt tctctgcttc ctctacagtt cggttataat tcttgtctct
    20821 cgataaagca aacagacgag agtcatgcat gatgtctggg atcgctacga gaatagctac
    20881 tctttcgaac atttgctttg gaccctctac tatttcatta ttaccatccc tcagcaaata
    20941 tcgagcagct aaaaccctca gagaattaat gtcaaaggcc ttgtctactt catcgaggac
    21001 cttcttgttt aaaatcttca tcttctcgtc gcgaagttta cgtctctcat gcctgtagag
    21061 aatgtacgct tttgctgtat cagacagacc ttcttctatg agtattgatt cgaccacgtc
    21121 ctgaacgtcc tcgacacttg ggatttcggt cgatccatag ccatgatgaa tcatcttttg
    21181 aactactttg ttggccagtt tttccgagag cgaaaaatcc ggtttaccag tagctacaag
    21241 agctttgtaa attgcatccg atatcttgag tggttcaaaa ttgacctctc ttccgtctct
    21301 ttttcgtata cgaataatca aattcttctc ttggtcatta aactcagtga cgcctgccat
    21361 tccgttatta cggcatgcta aatagattta aaagtattag cttcctgaaa aataattggt
    21421 taatttgaaa agaggaataa aaacatattt gacactattt gtactcattg ttcaaaatag
    21481 ttagtgaatt agcagcatat gggaggaccc acagaactcg catctttcct gatatctcga
    21541 acctgatctt tttccacagc tattgcatac catgagcttc accatcggac ggaaaaatgg
    21601 gatacccgac gctgcttcga ttgctttctt tagttgcaca tgattagaag cgagttctgt
    21661 tacatcaaga gttatagaca agccaccact cagaagcttg tcaactgaaa gacactcttc
    21721 tattagagac ccagaattat catcattggg catcagtaat tgctcaccat acaaggttaa
    21781 tccctgagaa tacgtcgtag agttttgttg agtcgactgc aaatatgccc tgccatattt
    21841 atcagaatcc aaagctgcaa atctagtagc gctgtcatct gctatcattg caacgccaac
    21901 cgagccttcc ccaatatatc ttccctgatc gagagcaacg tctacagaag tcttcagtac
    21961 cttttgtaaa acctccattc cattgttctt agaatggtgt cccaaaatat catttacaga
    22021 ttctcgggca cctataaggt taattatgat gttggtcgtt cctgattgaa taaagtcgga
    22081 attgccactg agggcaggag cagtgccttt tctaatgagc tctgagacag ccttctttct
    22141 tatgtagagc gccgctagcg ccggcttgat catcagagcg agtctagctc ggaaataagt
    22201 ttcatctttg ttcgactgat atgcaagcct tggaagatta atagagagcg attgcaaagt
    22261 tataacagtg ccggaacttt tgcctcttat tgatttgcgg ataccgctac tcgctcttaa
    22321 tccgtcatca tgtgaaagtg agataattcc tccacttctt accaaagacg cgatagggtc
    22381 tagatgatat ctaagttgat tttgctgatc attcatatca ccatagatta atgatatgcc
    22441 aatgcgtggt acgggagtag aatctacata tttctgataa ccgctcaata acgcggtaac
    22501 cgacgtcgca tcgtgtccat cagctggaat tgttatcgtg gtggcgggta atcctgactg
    22561 agtatagcta ggagctatcg aagacaacat aagagcctta gcaaatcgtg aactaatttc
    22621 agcaggttct ttcgtttgtt ctaaaatcat tggaataatc ccttcaagaa ccacttctgt
    22681 tgaagcttct ctgctcagaa gagaaatcaa taacgacagg taggtctcga agtcgttcga
    22741 gctattgata ttaggcaaag gcatccttga tacgttcaag aatttccctt taaggtccag
    22801 agcgttctca cggaagttag taacgtctag aaaaatcgtg tccggaatta ggccccaagt
    22861 gccgctattt gaaatattaa tctcaccagc caaatgcata tctgcaatgt ctttcggaag
    22921 agtattgatt aggaggtatt cagagaatat tgactgcgag gcttttgaca tcagagtttc
    22981 aagcccattc tttgccgcgt ctgcattact taacatctcg acaatgtcag atgcgggaag
    23041 gcctaatctc gccaatttat ttctatattc ctcatgccca tgttcaatta gaacagagtt
    23101 taccgtctct cgaattaatg atgacgtaag atagacggcc tgaaacttgt atatcttatt
    23161 ttcaacttct tctgttatct tatgtgcttg ctcaaggggc atatttgctt cccttactag
    23221 tgactgtatt atcttattag agttaaattc ttcaatggaa ggtcttgtag tcctaacata
    23281 cattttgcca ctttcgatta ttgacctttc ctctatctgt cttgcaaggc ttagcacgag
    23341 cttaccgaga ttagtaattg tatagcgacg ctctgctttg ttgagtgcca ccagggattg
    23401 tctgagaagt tttcgtaagt gatatgcgaa tttcccagat tcttttttcg atttaaatcc
    23461 agctagcgat tttaactcgg agtatgtaag cgggcccttg gaattcaaaa tgcgaagaat
    23521 gtcgattcga ttaggactag ccataacgga gaaaatcatc cggactcgtt ttgaggcgga
    23581 ttccagcact ccatctcttg gagattccgc ttgtattgga tatttatcgt gactcaactg
    23641 ttccattgca cccgatattc agactaactg attaaaacta ctttgtaaat tatatttaac
    23701 taatggtata gaagacagta atctaaagag gactaagttt aaccttctat cattatttga
    23761 cttttgtccg atacctcttc ctcatagaac tgcttatcct ttctcttatc cagaatggcc
    23821 gatggaactt caggaagttt tgtctttgct tcattctcga gtatcccttc tatctcattt
    23881 aggattgaaa gagcgtcttt atcaacgggt gtcgaaaact tacttccaac atccaactta
    23941 atatctgaat taactaagac ttcggaggtc atcgtttgca tttcggagaa cacgcttgaa
    24001 gccgctggaa ttaccttgga gatgtctctt tggatatccc tgatcatccc gatagtaggg
    24061 ttgatggttt ccaaaaccat ggcaaattcg ttaacagtac tgaaccgaat tagcatgacc
    24121 tctagtgcaa ggcctgcgtt ttgtgttgtc cgttgaacat tgcgaatatt tgataactct
    24181 cctgcaagag ctttagctcg ggagttattg ccagacttta tatttaatac tatttgatgt
    24241 ttaaaatttt tatccattct tgagaacttt tcatcgagca gccttaatcg agcgagttga
    24301 ttattaacta ttgacgtagc cttttccata tcgcttttga attgttgctg atgttgatcc
    24361 atttgtggac gaattcaggt taatcgaaaa aatgttaaga gcgagcgtta aactctatat
    24421 acaaatagct atcaaaaagc taatctacca aatgtgcagt agtccgacgc taatacagtt
    24481 cttgcttaac gtaatttgta ttagtaggat gctaatagca ttgcttgctg tgtgggtgca
    24541 actagcggca tgacacgatc tagacttttt gcaacgtcaa accatgtttt gggtgataat
    24601 ctaatactct gactcttgat ccgagcggta ggtcggaagc tgatgttact ccgggtagga
    24661 aaccggatac attgaaatct gtaccatcaa cactgaacac aacccacgca tatgatccag
    24721 catcctcaaa tccctccggt gctacggcct gaattgtgta cgtcacaacc ttacctgttc
    24781 cttccaactc gttactctca aatttgtgac taaagcattt ttcacagtaa tatactgtaa
    24841 caagcatcaa atgtccacaa cgcgagcatt tattggaaag tatcttgtgt cttcgcgcag
    24901 ccattataaa cttttcacga ggattcgaat cttgttcttg ctgtcttgtc tttgacaatt
    24961 ccattcattg aaccctctta aagatatgaa cggcagcact tgcaccagta gccccgaagt
    25021 tatgtgtaag tgccacttct gcatccctaa cagttctttg ctcagcttta ccagtaagtt
    25081 gttcgaaaac ctctacgacc tgaccgatgc ctgttgcgcc gattggatga ccttttgatt
    25141 ttagtccacc tgaaggatta atgggaattt ctccgtctct ggaagttgct ccctctctga
    25201 cggcattggc tccggctcct ttcttaaaga accccaggtc ctctatatca ataagttcag
    25261 cgattgtaaa acagtcatgt acttcagcaa aatcgatatc ctttgctgtg atgcctgcca
    25321 ttttatatgc ctggttggcc gcggctacgg tacttgggat agttgtaata tcatcacgac
    25381 cctggacggc agccggagat gcacctctac ctgagccaat cacctcaata aaaggcctac
    25441 cacttttctt cgcaaaatct tcactacaaa gtatgattgc cgacgcccca tcagaaaacg
    25501 gacagcagtc atagagcttt aaaggcgaag ccacgacagg agatttcaat acgtcgtcaa
    25561 tcgtaatttt tttcctgaca tgagcttttg ggttcaaaat tccattttcg tgattcttta
    25621 ctgcaacgcg tgcgaggtct tcctcgctcg ccttatattt agccatatac gacctagcaa
    25681 ttgacgcgaa taatccgggg aacgaagcac catttccccc ttcgtaaaag aaatctgaac
    25741 aataagagaa caaagtagta ctctggattg ttccagtatg cgttattttc tccacgccta
    25801 atcctaaaat gcaatcatag aatccagcgg ctacgttcgc gtatgcttct ctaaacataa
    25861 tagaacctga accacatgcc gattctattg ttaatcctgg cacctctggg agacccaggt
    25921 tactcataat gactggtgcc atatggactt gcctatcggt caccccaaaa acatttgaaa
    25981 tgtaccctgc ctggatatcc ttaggtgtaa taccagctga atcaattgcg tctatagacg
    26041 ccttcaatgc aatttcgata ataccttcgt ttaatttacc gtatttggta ctgcctgcac
    26101 cgagaacgca aaccttattt tgcatttatt aaaaactacc tgttcgagtt ataaaaaggt
    26161 tagagtaggc cagataacga tgaatattct gcgtcaccgt tctttatcaa ccagcgccat
    26221 ctttgttgag cagttgatca ttcgaatagc tgagaaggta gctgttactt gccttgtgct
    26281 ggagctagaa taggcaccaa tgtctattat aatgaaacaa gacagcgtga gcgttatgtg
    26341 aacgcactaa tatggttacg atacacgtcg gttcgacgat ttttcaaaag aggaagtgat
    26401 tttcgaacgt tttgtactct agatatatcg atttcaacaa cttccatccc ttctctattt
    26461 cccatgtcca atactaccgt accaaatggg tctataacca tgctccttcc ggagaagata
    26521 ctgcctactt gatctggagc cgccatgtag atgccattct cgatagatct ggctttgagc
    26581 aatgtttccc aatgttcttc cttcataact ccttgaaacc atgccgaagg agcggctaga
    26641 atttcagcgc cttgcactgc taagatccta gacatttcgg gaaagcgtat atcataacaa
    26701 atcatcaaac cgatagttcc agccgatgtg tggttaggcg cttcaatact atcgccagca
    26761 atcagtttaa gagattcttt ggatcctaac gcatcatata gatgtacttt tcgataatgt
    26821 gaaatgattt ttcctttact gtttatcaaa acggccgtgt catatacacg attttcagaa
    26881 agtctatcgc ttttttcata gattgttgcc acaatgttga tattataact ccttgctgtg
    26941 ctacatagtt tcaagataaa tttgccgttt ttcgctgact ctgctatctc cgccaattgt
    27001 ttcgaagatt gcctagctga aaacgccatc tgaaattcag ggaagcatat cagacgtgcc
    27061 ttcctttttg ccgcttcttt gataaaatct accgaaatct ttagattttc ttctttgttc
    27121 gacgatgatt tcatttgcac gattgcgaca cggtgtgact ttcccataat ctaccactcg
    27181 atcacaaaat taaaagtgtg ttggtcaaag aggctcggct gagataaata agttttgtct
    27241 tggcttcgtt aaataataca attacatgac caaagagctg tacagtaaga ttagtcattg
    27301 ccctttgcaa attcctatca gtctatagta aacaattagg caaaggtgaa ggtggcatat
    27361 agttgtcaat cctcttggta ctgctgagac gcgtcatcaa ccatacgcat gggaatcatg
    27421 tgactctact tcatgcttca ttgcctctta agtatacaca aatctgaaca aattcgtttg
    27481 ttactctttt ataaggagcg cataaattcc cccaactgca taagctaaac ctatcaagat
    27541 tcgagataaa aaaacagcaa gatcatgctt cgaaacaaat agagtcatat caaccaatat
    27601 cgaaatagga accgaagcaa gagaaatcaa accgacgatc atttgttcac ggttgttaag
    27661 gccattgtat tttttcatca gtagatatgt agacaaattc cctaagccta tgccgacgag
    27721 gatcaggtac tggtacattg acggaaacac gcctatcaaa gcgaagggag cagcccaaca
    27781 tagtcc
//
LOCUS       DAOR01000040            9865 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_11835, whole genome shotgun sequence.
ACCESSION   DAOR01000040 DAOR01000000
VERSION     DAOR01000040.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_11835"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2925 a         1986 c         1955 g         2999 t
ORIGIN      
        1 ttacagcagc tatggaagtg gatcggcatt atcgaagata tagagaaact agaggtggtg
       61 gtatgaataa ttatgaagac aatagcggtg accgtacgca gataacaaca acaggtgata
      121 ctcaaaagcc agtttttaat aatcgaggtc actagtatga gtagaaaaca ggtggtggta
      181 gcttttggtg cgcacccgga tgatatagaa ataggcatgg gaggaacaat agcaaaacta
      241 agtgcgctgg gatatgacgt aaatctggta gtagctacgt tgccgaactt tgtcaagata
      301 gacaaaaaag aggaacggcg aaatgaatca acaatatcag cgaaagtgat gggatgtaaa
      361 actccagagt ttctggattt aactcctgac gaaatcacct ttggtagacg tttcgtaaca
      421 aaggtagacg aaattatcaa tagacacaaa cccgaggttg tttttactca atggatagga
      481 gactctcatc aggatcatca agcacttacc aaggccgtga tagccgcttc gcgtgacctc
      541 aataatttgt tcatgtatga aacgactata cctggaggca taactgaaaa tgccttccgt
      601 cctcagttat acgttgatat cagtgcaacg ctggacataa agacaaatgc tcttgattgt
      661 tttgactcac aaaaaatgcg ctgcggccac ctgtgggttg atgcaatagt cggacgatgt
      721 gcatacaggg gatatcaaaa taatacaaga tatgctgagg cttttgaagt aatcaaggtc
      781 tccaagtggt agtaagctgt cccaagtgat ttcaaattaa attatctgaa tatttagaaa
      841 taattcaatg taaatgaatc attagctcct actgtacctg tactcattag gttttcatat
      901 gtaggaaaat cggtgtaaac ctcgttccaa tatctcatgc agactaactt gcgatagctt
      961 ctgactttga tttcttcacc cagaacagag ttacggaaca cgatatctgg ctcgtttatt
     1021 cctgccaccg aacgcatcgg acaagttgct gaaaaacgtg ggttgatttc aaatatttta
     1081 ggcctattct ctataagttt ggcctgtatg tttattgctc cccttgcacc tagtttcaaa
     1141 gctgcttctt cagatgatct ccgaacttct ttataatcat caataaaggc tttgtaagtc
     1201 tgtccgttct ttagtgtttt ctgaattgct acagatgaca ttacatattt tccaaaccta
     1261 tcaattgtaa cgccagaagt atattccgta ttattctcgt tcaagtattc ttggagcaca
     1321 gcatgccaac ctgctttttg tatatttgaa atggcttgct gcatttcaga tttgcaacgt
     1381 actaaataaa aatacaacga accatatccc tcccttggct ttacaacaat aggataaccg
     1441 aactccttca cgaacttttc atcgtccgcc ggtagagcag attcagcgac cggtaggtta
     1501 ttttctttta gaaagagaaa ggttttccat ttatctctag cagtagttaa tacgtcgatt
     1561 ggactcgaca gcactttcgc tttgctttcg ctttcaattc gtttcttcga attaccaatt
     1621 attaataact cttcatccga tcctatgtaa atcgcatcca tgtcttcgtc tctcgcaatt
     1681 ttcagaatac tttcaatata atcggtggag gtagccgatg gtagcaaaat accattatca
     1741 catctgtata atcccgccgc ctgagcgctc atgtctccag cagtgattct gtatttcact
     1801 acggcattat ctgacaaatt agcaagtttc agtgatttga ttattccctg cgcgacgatc
     1861 cctcctgcag ccgtaaccag taccttggca ttcattatag gagacaccct atcttattca
     1921 ctaaaaattc attcgctatg gtgacatagt tatcgatgct gataaactat cattctttaa
     1981 cagatttcgc cgccacagcg tttcggtatt acccacgccg aaattgattg catagtctat
     2041 aatagatggc tttgtttctg ttgcattttt tactttctcg gcaactttta tgagttgacg
     2101 tatttttcgt tgctcttgct ttaacctaaa caaattcgat agtaccgaat cgattgatcc
     2161 gccagctctt attagacgag cccgctctgc tatcaaagga tctattttac tgttgtagtc
     2221 taaatttttt tgttttagga tctccacata attagaaata tccgcttctt ttgataatgg
     2281 gagtgtttct agagcctctg cttgagctag gccacggtag acatctgtgg cagcccagac
     2341 taaggttaca ggcattttaa tagatagttc cttgaagacc atggctccga ccattgtgta
     2401 ccccatacta ccgcctgttc cagaggcata gcaagtgata ccgagatcac gtgaaagcaa
     2461 tagaagaatt gggattgccc taggagataa gccttgaagc atatcctttg aaatcgttat
     2521 gtcattgtga tttcctaggt ttacctcgat agcctttttg catgacatac aggtgcctgc
     2581 tagcgatatc tgatcttgct ggctcctgtg cactcttgct gaagccttac ttccgcaccg
     2641 gcagtgcacc cacaaaggtg catgcagata cgaggtcggg ctgactcctg tatctatgcc
     2701 acaacggaag aacatatttt cggttttact caacacgtct gaatatttca taaaattaga
     2761 aatcaaatac ttgaaaccat cttcaaaaac cgtagacatc tcagaaagtc ttacaaacaa
     2821 tgtatcatag ccccattctg cattaaccag ttgtgacatt aaaaaagaat taaaatctga
     2881 gtaggatttg gaccttgagt acgactcttc gacgttccgc caaaaatgct caaagttatc
     2941 caaaatcata gatttatccg atttcgaata taccgatgaa aatgaatttc taacccacga
     3001 ataaatctgc attctccaac gatccaggat tgttcgtccg ggcaacggca tattacatac
     3061 tagctgccat cttctcgatt cgctaacggg catccgcagt tccagcctgc catcggagtg
     3121 ctttatgctt ggaagctctg ctaaacggat atacgcgtca tccataaaat catgatctac
     3181 aatcagaaac aaatttacaa atttgttaag tgatttacgt tcttcaacag ccctcatgag
     3241 tgtttggaga agcacaattt ttttgaacac acctccatat gcaaataagt ttggttgatg
     3301 aatggataca aaaatttgtg tttttggatc gattaataat ctcagcttgt tagtaacgcc
     3361 ggcagtcatt gtggccgcct ctctatgaaa cttgtcgatc aatatttgca acttgcgccg
     3421 attattcagt ataaaatcgt tagtagcgat atcctctgtg tgtgtcaaaa ttttattgac
     3481 agcatctgag agcttttctg gtatagaaga tatatagtca gaaatgattg gttctccgac
     3541 attttcatat tttgaaaata gcgatctcac ctgaggcgta cgtcctagcg ctgcagtcgc
     3601 catcggatct cccactaaca tacacaatcc caacgtctat ataaattacc attcggatct
     3661 cgaatgcctg atcacctttt taaccatggt ttctgtgtta aacagtgtaa taagtaatta
     3721 caagcttttc gcaggaatac ctttttatca aaatgtcaaa tataaaaaag atagaatatt
     3781 cgtagtatga agtcgtagca aaatcattgg attagtatta ttttattatt gcttattttt
     3841 tgattagtat tattttttcg aagactacta agctgactaa tgtgaatcct gattaatgac
     3901 cagcgagatc aaatccgaaa tttttgtgaa ttgccctata tcctccagac gatgcgcatc
     3961 gctacccaga tggaatttta ctcccttttc catgaatgga acaatccact caggtggagg
     4021 cctgcgatat ttagcgttaa tctcaattat ttttttgttt ttaactatta aggaacccaa
     4081 gtcttcaact tcatattgtt ccattctaaa cgtagtttct ggagcaatgt gcccgataac
     4141 atcgacgcta ggattttcga tagcatttgt taatgcattt agccatacac tcttgtcatg
     4201 atagagagta tgaaaacttg ctattaaaaa ataattttta ctgtaccctt ctggacaatc
     4261 aatagaacca tccactaaga ttttcgcctc gaaccccggg attattttta ctgaactatc
     4321 cttactggac gattctatct cccgcagata ttcggggagc caatctgagg tctttctgat
     4381 gtgttcggta aatgcaatcg tttgtaggtt caactcctct gcttgccttg ttacgtttgg
     4441 gatcgtaaga tcatgtgctg aatggtcatt ataattgcag tgaacatggt aatcttctag
     4501 taggttcata gtatactata ctgatctttg ttataaaaaa ttttaaccag ataatacgca
     4561 acagactttt gcgtaaatac ttataagtta tataccttat acgactgtac taattggtaa
     4621 tgccatttgc ataggttcat gacgtcgaca gttctagtcc cgggtgctgc cggccctgcc
     4681 ggaataaaca ctatcaagtc tttgaagatg gccggcttcg gagggaagat tcttgctact
     4741 gactccagct cgttgtcagc aggttttttc atggccgacg taaatgaagt aattcccgag
     4801 gctgacgacc actctttcgt ggatagattg tttcatattg taaagatgta tcaagttgag
     4861 gttctgatgc catcttcagg tttcgatatt tacccctata gcgaaaaccg aaaacaactt
     4921 gcggagatgg gggctgaagc gattgtaagc gacagagata agttagaaat ttgtcgtgac
     4981 aagatgttaa cgtttcaaac tctttctaat aaatttattc ttccatttac tacggaggaa
     5041 ccaaacaaga tagacatatt tccagtactt gccaaacctc ggttcgggaa gggaagccgt
     5101 ggcatagtca aaatcgatga tgaatccgat cttagataca taatatctaa acgcgacgat
     5161 ctcgtatttc aagaatatct accaggaacc gaatatactg tagatgtact ttctgatcta
     5221 aacgggaaac cgttaatcgc cgtacctagg atccgaatgc aaacaaaggc aggtatttct
     5281 acaaaaggta gagtactgcg agatccagcc ttggaaatcg attgtatgaa aattgcagaa
     5341 tacattggaa ttaggggtcc ctgttgtatg caaatgaaag agtctgcaga gggcagactt
     5401 aaactgattg aagtaaatcc aagaatggga ggtgggacca tatttactag cttggctgga
     5461 gctaactttc ctgcactgtt actagatatg atcgaaggaa ggcagatttc ggtaccaaca
     5521 atttccgaga tcaccatcat taggtacttt gaagaatttg tattgagaag cgaggaggag
     5581 agaatacatg ttggagccaa tttgttatcg aagacacgtt ttcagggata gacttatcac
     5641 aagacacctg ttggatatgc atgacattta tcaaatggca tggccaataa cctgtaattt
     5701 tcatgacaat aatacagaaa cctatcgtca ttccaatact tactaagatc gcatcccacc
     5761 ttctattttt tttcttactt ttctctgtat gtctcattat cctttcacaa aaacctttgt
     5821 aatctaggca actccaaagc taattgtgag aaaaatattg tttggatata ttccgtgatg
     5881 tctaatgatg ctgttaattc cccaaatctg agctgctggc gagaacaagg ttaaaaccaa
     5941 attattaacc tcatggaacc ggctgggtgt ccatacattg tttaatttct atgatcaaag
     6001 atatatctta tttactgaat caatctgtta atatccgtta atgttaaaaa cggaattacg
     6061 tcaggtcatt attaattgag tcggcgaact gaagaatatt gctaggcaac tcatgcttct
     6121 gctaaaattt gcccagctat tcagtctata cagagatttt tattccccat ctcaagattt
     6181 gctcggtgtc gattaccttt tatatttgca tcgtcccttc cggacgtaca tgggtagggt
     6241 ggagaaagga gtgaattgta gcgttcaagg ttgcgagcaa tccgcccagc gatcaatgag
     6301 cggtagcaag gcacgaatgg catcaaattt ggttgtcaat tcggacaaac gtgtttatct
     6361 ttgtaaaacg cattacaaag agtggaaaaa agctacaaaa ggcgatcgag agaacgagag
     6421 ggctcgatgg caataaaaag agagaacctt tatttcctat ctgactatac tgtcttcgtc
     6481 taataaatgc gcttacttca gcttcattct gatttcgtag agtatcaacc catcgccaag
     6541 gagatcagag aagccgaaga aaatgtctcg agtagcaaag ttcgttttga agacctcgtc
     6601 gttagtcttg ttgcaatcga gaatggagat gacgaaaacc tggctaagat tgcagttaat
     6661 gaaatagaaa gatatctcgc cacagtaaag agcaaaagat tactgattta tccatacgcg
     6721 cacctaacat ctgatctcgc tttgcctgaa cgtgctctgg aagttatatt gtcaattgag
     6781 aaatttgcga aggagcgatt cgtggacgtt catagagcac ccttcggatg gaccaaggct
     6841 tttgaaatca aggttaaagg acatccgttg gcggaaaatt ataaaatagt aacaaaacag
     6901 gcctccaata ttgctccagt tgttgccagt aacagcgacc gtataattaa ttcttgggaa
     6961 acgaaggttt cgtctgcact tagagaagaa gaaaaattat cttcgatctg gtacatctta
     7021 gaaccagacg gaaagctgtc acctatcaaa gactacaaat tcaaagccgg agaccaaaat
     7081 ctgcatactt tagctaacta cgaaatggcc aagaagaggt taacggtcga gcagccagct
     7141 catgtcagat taatgaaaag gatgggaatt gcggattatg agcctgcttc ggatgttggg
     7201 aatctgcgtt actatccgaa gggtaaattg atgaaatcgt taattgaaca atatgtcact
     7261 cagcaggtaa tggaatatgg aggtatggag gttgaaacgc ctataatgta tgattccaaa
     7321 catccatctc tggagagtta tttcaatcgc tttcctgctc gccagtatac gatcacaagt
     7381 gataacaata aacaactctt cttgagattc gcagcatgct tcggccaatt cttgatggct
     7441 aaagactttc agttatcata taaacatctg cccttaaagc tgtacgagct tactcgctac
     7501 agctttagaa gggaaaagag tggggagctc tcaggtctac ggcgacttag ggctttcagt
     7561 atgcctgact gtcacgcgct atgcagcaat atggagcagg caaaagagga attacttcgg
     7621 cgatttgaac tatctaccac cgtgatcaat gcactggggc tttctacgac aaacgacctc
     7681 gaaatggcaa tacggtttac tgaagatttc tataatggaa accagacatt cataaataaa
     7741 attgttagca gatatgggag gccagtgtta gtggagatat ggaaggatag atccttttat
     7801 tttattctaa agtgggagtt caattatatc gatagtactg gaaaagcatc agctctttct
     7861 actgaccaaa tagatgtcga gaatggtagc cgttatggaa tcgaatttgt tgacgagaat
     7921 ggcaagaaga aaaatcccat tatattacac aactctccca gcggcgcagt tgagagagta
     7981 atgttcgcac tattagagaa ctgcgcaaaa gttgcaagag atgggggtaa gccgtctcta
     8041 cccctttggc ttgcccacac tcaagtgcgt atcattccgg tgagcacgca gcatttagat
     8101 ctgtgtagag aaatctatac agaactttca agacggaagg tcaggacaga tatagatgac
     8161 cgaaatgagt ctgtcgcaaa taaaattaga caatctgaaa ccgaatggat taactatacc
     8221 atagtaatag gagataaaga gctgcagact ggatggtttg tcgttcgcga tcgaaatcaa
     8281 ggggaacagc gcaagatagt gttggcacaa cttggagacg aaataaattc cttgacaaat
     8341 ggaaagccgt atattcctct aaatcttcca ccatatcttt ctaaccgacc acaaattatg
     8401 gtttagttac cagtacaaag caccttacac aaatataatg catctggaat agccggcttg
     8461 caacagaact gagttcactt tgatgttcta aaatattttt cacaataaca tcaatgaatt
     8521 taagagtagt tttctcataa tcatatccac tcttttgatt taattcaggc ttcgtctcta
     8581 actaacggac ctgttgtcta tgccttaaca taccggaatg tttctttaag tcaggcagtc
     8641 gtgagagtat acgaaacaaa gtttgtgacg ccaatgttcc ttcacgataa tcaccatttc
     8701 tcaatcccac ttcaacgata tcgatcccga tgagctttgc ggttgagcat atattcttaa
     8761 gaattctcct aacttcaaag ggatccagcc cgaatggttc tggtgtccct gtacatggta
     8821 catagggtga atcgtaaacg tctatgtcaa aagatatgta gatatttaca ttacgtgttg
     8881 cttttcgaat atactttaat aggttatcaa ttctttcatg tacttcccac gcgtcaaacg
     8941 tcgtcacctg attttttttc gctatttcat tttctcctcg atcagtctgc cttattccga
     9001 tttgaattat attctttcct gggatatatc catcattcat aatatggtaa aaaggagtgg
     9061 tatggcacat ctttattccc tcatattcag gtttcatgtc cctgtgggca tcgaagtgaa
     9121 ttaccaatgg tttttcgtcg gaaagcgcct tgagagtata gtaagacaga gtatgttcgc
     9181 cacccatcag caccggtact ttatttgcat tatgaattag agaagttatt ttatgaatct
     9241 tgctgcctag atatgacaaa ttggacgcaa gattattagg acttcttttt ctcatgttct
     9301 tgactgatgc agggattttc agatctccta tatcaaatat tctcgctcga tcatagatat
     9361 ccactttctc gtccaacaga aaagtttcaa tttggcgtgc cgacgttaat ctaattgctt
     9421 ccggtcccct caccgtccct tttccaaaac tggtcgtgat atcaattggt gcaccataga
     9481 atacgacatt agatttttcg aatgatactt taacaggtat atctgcgaag ctataccttc
     9541 ctctcgttct ttctggagga atgaacagag tcgagagaac gtcgagataa cttttcaata
     9601 ttcatcttct tgtaagtatg tgaccttgtt taagtttctt attttttgct gcagcatgtt
     9661 catcgcgcgc gtcgtaacgg ttttcgacac gcgatgcata catacaaaaa cctaaagaga
     9721 cccgttcaga taaaatctgc agtgttgcat aacttgtgaa cctactatat gatattgctt
     9781 ttacaatcta ttttctttga ataaacgtat tgtataacga tgcctttagc atcatttctt
     9841 tgaccatgtt tggaaatctt cttgc
//
LOCUS       DAOR01000041            5515 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_46080, whole genome shotgun sequence.
ACCESSION   DAOR01000041 DAOR01000000
VERSION     DAOR01000041.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_46080"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1905 a         1003 c         1048 g         1559 t
ORIGIN      
        1 ggagtgaaca caccggtatg ggagtttatt gctcccaacg ttactgcaga tacactcctg
       61 cgatttcaac taaatgttac ggataacttg ggacagatgg gcactgcctt cgtaaatgta
      121 gtcgataagc cagcttccac cttcattgct ccaccgccga ctcagatcat gaagtcacca
      181 tccacagctg caactgtgcc ttcgaataac ccgaataaag gttcgaactc tgtcaatatt
      241 cctactaaca acgcatcgat ttcaccgcct ttgactccac caataaatca gaggagtaca
      301 agatgaccaa cgtttattga actttcgtca aaaatgcttg atgttagata gagattggtg
      361 actacagaaa taacgttatt gtgccaaaaa cattattttc tgttgtatcc taggccaatt
      421 cgaggatagc gctgatgata ttgaatatgt tatttctgtt tttagcaagc actcttttcc
      481 caaatgcttc ttatttaggg ctattcagag ctgtatattc ttgatatcca gtaaagtcaa
      541 gtggttatta ccctgtcttt tatcccctct tgaaccccat caacagttgg aaagaacgcg
      601 taccattcaa atcagagata tgatatgcaa cataaaataa caagttaata gagatcagca
      661 cgaaatgtta ccaataatat ataaaaacaa tgttgttatt accacattag atccgtgtct
      721 tcagaaagaa tagtttcatt cttacctagt tctaccgaaa tactttatga aattggtgca
      781 ggctctcaat tagtaggtgt aacgcatgag tgtaagtatc ctgacgaggc aaaaagaaaa
      841 ccaagagtaa taaacgcttc ctttgatgct agtaaaatga atagtaagga catagatcag
      901 aaaattatag aactaatgca atctggcaga gatatatatt taatagacga cgcgaaatta
      961 agagaagcaa aaccagatct cattattgct caaggtgtat gtgaagtatg tgctccattt
     1021 acaaaagaga taaaccgtgc tacttctgta ttgggttaca acccggatat ccttgtactt
     1081 gatcctcatg atttagatga cattttgaca agtataatgg atatagccga aagagtcggt
     1141 agagtaacag aaggacgtaa attggtagta tcattacaaa agcgtattga tgacataaga
     1201 ataagagtcg gccataaaag aattggtgcg aatgtttcga ataataaaca taatagtaaa
     1261 ccaaaaatac tttgcattga gtggattaat ccgttcttta ctgctggtca ttggattcct
     1321 caaatggtag agattgcagg aggtattaat ggtgtaagtt cccgcgccca accttcacgg
     1381 cgtatgcacg agattgatga gattattacg ttaaatccag ataaaataat tctaatgcct
     1441 tgcggttttg atatacatag aacattaaga gaagcaaaga cattagaaac taacaataaa
     1501 tggaaatcat tgatggctgt tcaaaacaat gaagtgtatg cagttaatgc aggagcgtat
     1561 tttagtaagc ctggacctag aacaataaca gggttagaag tattagcaaa gataattgac
     1621 ccagaaggct ttgaagacat taaagtacct acagatacat attgtaaatt aattaatgaa
     1681 tacaataggc ttaaataact atccatactg taaagtacgc gattgcaagt tcagtcatat
     1741 attcagtcat acatccaatc cgttgaatgt acgaaccatc taactgctga aatctatagc
     1801 aaacgcataa agaagttcaa cgctttcgcc gaatcggaat ataaagtcag tgctgataag
     1861 cttatagaaa taatcaaaaa tggaaaggac ccctataatg caattaacaa atatgtatat
     1921 tatttgaagt ataatcacaa tctatctcct ttatcgctga aacaagaagt aataacagtc
     1981 aagaatttgc ttgagtactc agatatggag attagcccac gcaaatttaa gctcaaagta
     2041 aaactgccca aaataattag aaaaactaag caacctttat caaaagaaga tataatcaat
     2101 attctaaatt catgtgaaat acggctgaaa acttacgtca tgcttttagc ctcgaccggt
     2161 atgcgtgcaa ctgaggctct gtctatacga atcaaagact tggatttgga ttcaaatcca
     2221 gtcaaaatcc aattacgggg agaggttacc aagaccaagg tagacagaac tatttttctt
     2281 acggatgaag tttctaaaca actaaaagcg tggatagact acaagcacag agctaggcgc
     2341 gtgtgtcatt ccgatacaat atctggtaaa tgtacgacta gtatgagaac accggaaaag
     2401 aacgaaagtg atctgatttt tgcagctcgt gaaagtataa gcgttcacaa tgtatatatt
     2461 aatctgtgtc ttggttttga gcgcgttctt gacaggctac atatgggtca aaaagaagaa
     2521 tttaatgtta accacacttg caataaaaaa caccatagac gtagatgtat aactttacat
     2581 tcattcagac ggtttgtgaa gaccactatc agtgatcttg gatacggcga ttttagtgaa
     2641 tattttattg gacattctgg atcaacatat tatagaaaga ctgagaaaca aatagtagaa
     2701 atgttcaaga aaatcgagtc acatttaaca ttcttggatt atcccactct agaacgcaag
     2761 ggtgctgacg ttcatgccaa actggatact ctagaacaag aaaatcaaag attaagacat
     2821 agcgaccagc taaaggaaga cgcactggca acattatcag atcaagtaat gaaattaatg
     2881 gtggaagtac aggaattaaa gaagccacat caatcataat attagctagc tcaaaacaat
     2941 cgcaataagg agtagatgaa ttccaaatga ttaatcaaaa aataggcaaa ggtgataata
     3001 tgaataccaa cgctattgcg actctatcag atatatcagt tcaagtaatg caagaaatag
     3061 ataataaaga agcgaagata aaattacttc agtagttcgt cagtacgaaa atcaaatgtt
     3121 ctatggggct cctcttcatc atctggacta ttgtctacac tcgatgctaa catccctgtc
     3181 caagccaacg ccctgctgaa atcgtctagt ataaccttgc gcacgttttc cttcttgaag
     3241 tcaactaaca tagttctgcc attttgtttt ttatagccgc ctgctaagaa cgcagagact
     3301 ttattcctca ctattctttc ggcgtgatga gttaatatga gacgaaaatc atgttgtatg
     3361 atttcttctc cttcaagatg taaatcatcg actttcaaaa tattgcgtaa taagttgaaa
     3421 aacataaaac cgcattcgtg ctttgcagat ttgttgcgtc ttacgcctag caaatctcta
     3481 atatcatctc cgtataagtc ttcaagttct ggatgctttg ctatggcctc aaagaagaat
     3541 gccaagttag ccatggcttg aaaaatgtgt ttgtcaagat aatagacctt catcctatct
     3601 atgttgccct tctttctttt ctccttatca tcttccccta attctttccc tgcctttgtc
     3661 gccttttttt tatgtaattc cttttttaaa agatccctca ggtcctcgtg cggagtgtaa
     3721 tattttattc cattttgctt tataataccc attatatgta agtcggaacg tttaccttac
     3781 ataaatattt atcatctagt gttcatgtat aataaagaac caaatgcgtc tcgaaaaaaa
     3841 attacaacta cctttcttag cggtacgact tcctgtactc taattatagc caaggaaatc
     3901 gcaaagaaat acggaataga tgagccttca catgtgattg tagaaggaac agaagagggt
     3961 attctaatcc gaaagctcga actagacaat aaagatggga taaaactcgg gttggatagc
     4021 tgtagatgtg ggtgtcataa tgtacagagc ccaattgagg tgcgagattg atgaacttaa
     4081 gtgttgactc gattcacgat attgacacta atcattattc atcatgtgaa ggcgaaatgt
     4141 ggactgaagc ttacctggtt gatcgcctga aagaacattt agctacagtc aagcgacagg
     4201 catctgcaga cacagaaaaa ttttatgata gtcgaaaata ttatagttta gctcatcacc
     4261 ttttggtatt gggagctttc agcccagttg ggccttttcc aatcaacgta tccgacttat
     4321 cggctaaatt gttgcaagaa ttaagccagt tagataaaaa caaccgcaaa tatgtactaa
     4381 aacaagcaat aggccggatt tttgaagata taaactgcaa gttataccac gatagtattt
     4441 cccgtgtttt ggaggtgatt ctagtagatt gaacgaacca tctaatactg accaaagcaa
     4501 caaggacaca aacgatgaga tagaacagat aaagaaaaaa ttagttgcag atcagcacgt
     4561 cgacacatca tcagataacg acaacacaac agctttgaga gaagaactag aatccgaggt
     4621 acctgataaa gactttgcag agtttgtaat caaaacaatc aagcggactg taagacagga
     4681 agattcattg gtgcgtcaaa ttttttatac agggctgagt aaggattcag ctaatccgct
     4741 aaatctagct gttttagcac ctactagcga aggcaagact tatcctatgt tggagtcatt
     4801 gcagtacttt cctaaggaag aaatctggaa gattggttct atgtcaccaa aggtcataat
     4861 tcgccaaagt ggaatattgg tcgacaataa caatcagcct ctagcggata gaataaaaga
     4921 attgaaaaga aagattaagg aagtagatga caagagtgat gaaaaagagg atttagagca
     4981 gcaattaagt caactgtaca atgaggctaa agttctgatt gatctacgag gtaaactatg
     5041 ggtatttttg gaaccacccc actcagagac atgggctata ttaaagccaa tattatcgca
     5101 tgacaatctt gagatcgaac acccttatgt ttacgaggta gagggtatgg gattcaaagt
     5161 aaagaaagtc gtaacgaaag gttggcccgc ctgtatattt tgtagtgcaa aaaacgagtc
     5221 aaactggcca gcctggcctg aaatccaaag tcgatttttg ataacctccc caaatatggt
     5281 tcaggaaaaa tatctcgatg gaaatagatt gatagctcaa aaaatgggat tgcctacttt
     5341 attgcaacaa tcaataataa tttcaaatag ccaaatcgga ttagctaaaa aatgcgtttc
     5401 ctatattctt cagcaaatga ggcaacactt tataaaaaac acaatgaatt tcgataccaa
     5461 taatgttaac cctgtttgga ttccgtatgc tccaatattg gcggaggttc tgcct
//
LOCUS       DAOR01000042            2652 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17296, whole genome shotgun sequence.
ACCESSION   DAOR01000042 DAOR01000000
VERSION     DAOR01000042.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17296"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          807 a          517 c          495 g          833 t
ORIGIN      
        1 cactagtagt accagtattt accggtgccg catataatta cgccttttac atcttctacc
       61 agcattatgc gggtgtactt aacgtgactc gtcatctctc tcttttatca agcttggtta
      121 cgaatcccaa atcaccacac tcggtagcca ttgatgcttc tgcatacgcg atgcgctatt
      181 tggccaaaca tctgacattg ctgacaccaa aaaataatgt aagcgtcctg acagatatca
      241 atgtggatgg aggttccatc tttatggaca acaaaaatac atctaacagg tatgatattc
      301 tcatccttgg tcatcaagaa tacgtaacac agcaagaata cactaaccta aaaacatttg
      361 tagcaaatgg agggacaatg attcttcttg acggcaatgt attctacgct caagttggtt
      421 acgatagaag cacccaaacg ataacgctaa ttaaaggtca tcagtggggt ttcaatggca
      481 aatcagcatg gaaaagtatc ccagagagat ggaagaatga aacttcacaa tgggtcggca
      541 gtaattctct ttgctcttcg tgcatagtaa gatttgccaa taatccgttt gcttaccacc
      601 atcatgagga acagtatatt actaatccca atgacactat cttgttaaat tataacgcat
      661 cagtcttgag cctgaatccc aaaactgcaa aaaatgctac ttctgcacac tcgcttacga
      721 ttgctgccta tgagctgaac tatagaaagg gaacggtgat cgtattggga atgtattcgg
      781 atgctattat agaccaccct aacttcctcg tttttttaga tcacttatta atggcaaggc
      841 agttcaacca ataggtaaat gacgggataa agacgaagta gcatgtgctt cttcagcata
      901 ccacatactg ctaatagtag gaagtctctt tgtatcagtt gggagattat tgagcatggt
      961 taattatatt gccaatctta tcctataatt ctctgagatg ttcatgagca gttcgacctc
     1021 aaagtcccgt tacggcaaag atcaggagtc agtacatcac tcaaacctct ttaaagaaat
     1081 atctactttt attcaactaa tttttttgtg gcgtgaaata aacattgttt tgtgtctact
     1141 gacttgagcc aagatgcctt cttcatgata ggaattcgat gaacattcaa tacttctggc
     1201 gtatttctat ataattgctc atattggtga atgaaattat gtggtgctgc cttaattggt
     1261 ggttgttgtt cgtaccaatc aatgttgact acatgtttgt ttgacatgct aatcagtttg
     1321 ctcataacct cggcaatttc ggatggcagt atgtgcataa gaacttcaga cgccattacc
     1381 aaatcatatt tcttttgaat ttgaaaagat tgtatgtcag aaactaaaaa agtaaggttt
     1441 ggaacctgcc ctttcgctgg tttaatccat tctttagcat tttctatttg atctggcgaa
     1501 aggtctatgg caagatattc cataatattt ggaaaatttg atagcaataa cttcgtgatc
     1561 cgtccgaatc cacaacctac ttcaagcaca gtcgaaaaag aagacgaaga aacattattt
     1621 ttcagataat caatcagtat tttctcctgt agttcaaagt ttttgttata tcgaaattct
     1681 tttttgtatg tctttccatg actaaaccaa tattcatgag gaatatatga catttagtaa
     1741 aggccggatg ttattctact taagggttct taggcattac gtagaatgct gggtatgagg
     1801 aagcctcatt atgaacaagt aatttttgct cactacaaat tttcccctaa atccatcgta
     1861 taccaagctt actaaacaaa gtaaaccgta agggataaag gaacaatgcc agattttgta
     1921 gcttctatta atacaatttt tgggatgcct cctagaacaa aatccgaaat acattcagga
     1981 taagcaacac ctctgctacc catcttaaat cctggtttcg gtaatttgtt attaagttgt
     2041 cattcttata atcattatta gattacacga ttgtcagcat ctaaactatc ttgagccaat
     2101 actaaacagg tatggtttca aggattcttt ctttggagtt tgtacttggg taggaaaaac
     2161 aaaagacagt cagacttggc aatatatagc aggatgatgg aattgacatg gaatcgcaca
     2221 ccatgacgca tgctcatcaa caagccaact gactgaccgg atcaaatcaa tgtcttacta
     2281 accacgcggt taatgcaaca attgttgggt atcctctaaa ctgaaaaacg atctatcgtt
     2341 aacgtactta gggggtggtc gttaggttaa catgaatcta gattcactat ctattatgtg
     2401 aaattatcat ttagtttggt tttgttgcgg ttttgctatt ggtaactgga aaaaaggaaa
     2461 tgcaccacta ccaagagcat aaggcatttg tccgttccac cggttaatcg ataaccactg
     2521 aaggtacgcc ggactctgtt ttaattgtac agtaattatc ttgatagcct gagattcgcc
     2581 attcgctttg gctacattgg cccttgctgc tgcctgagcc tgaacaactg tttggtttgc
     2641 aactacttgt at
//
LOCUS       DAOR01000043            6369 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_43487, whole genome shotgun sequence.
ACCESSION   DAOR01000043 DAOR01000000
VERSION     DAOR01000043.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_43487"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2025 a         1198 c         1129 g         2017 t
ORIGIN      
        1 ctatgtaagt actacaccta gtaacctgac gtaatacgga actcaatcca tttatgtaat
       61 gggtcagagg cttcaggttt tctaatgcta tctagataga gaagtatgtc ctcttccgtc
      121 atatccttga atggtttatg attcaaaaat tttgaaagca caatcgacgt cgatataatg
      181 ttaagtctat atgtatcagc aatattgact tctgtctttt gtgatagaac ataattgaca
      241 attgtgaggg cattttctaa tgatagtcta cttagatcgg tcaggtgtct atggacatat
      301 actgcgagtc cttcagtcgc caagtctatc ctccttgtat ttacttcata atcagatcct
      361 tgttgtggtt gttgaaggtg atgtgaatgc ttggctatct cgtaatggga ccttacttcc
      421 attcttgttg acattgggtc agccttcgat gcacttgacg ctattaccat ttattttctt
      481 cttcgatatg atatagtata tcgtgcataa aatacattct attgaacatt atttgttgta
      541 acaaaggcaa tctatactgg gttaaaccat agctaataat aatgatgaga tgtcattaga
      601 gattgtaata caatgagtga agacaagtca gaacaagaat tggctaaatg gcgcaaaaaa
      661 atagaaatga taggtattga tatactaggt ctagatcaag agacagaaac agggcggttc
      721 aaactaaaaa caaagtttat gaacgaacat tttataaatg aattagataa aataggtttg
      781 caactcataa acattcgagg aacatttatt ggcagacttg tggtactttt agaagcaaaa
      841 aatgtgaact ccatgtcaac taatactact acttagtacc ttaactaact ataagcgtcc
      901 tttaaaaata atggtttcga ttattgtctt gtcagcctta attctattat gatattatca
      961 tgaaagttct aagctattgg aaatctaaga gtagatttga tcaatcttac gataggtaat
     1021 gtaaagggaa tgaggatcgg taccaacacc gtctacgaag agaaagtcac tggtgacgtt
     1081 attctttctg tgactatcag ataaattagt ttcagatttc atagaagtaa tattttgtat
     1141 tatagtcaaa gcattgtctt ttccttatcc acccacataa tctttaatgt tacttatcat
     1201 actgccttca ttcttctaaa tgaataagat ccgaatattg caaaggcgat cgtaaacgca
     1261 gctaatacac ctacatctaa agcgaatgaa tggatagatg ttccaatgat ggcatttctg
     1321 agagcatcaa cagcataggt agcaggatct atagcggtta gaaagaaaag ccaacctgga
     1381 aggttcttga gtggaaaaag agctgcactc aaaaagaaaa gaggaaatac tacaaagcta
     1441 attattaact gaaatccctc tagactttca atgtagctac caatggcaag tccaactgag
     1501 gttagggcaa atgagaggag tagtacaact gcgatagtct gaattaggga taatacatta
     1561 aggttaatcc ctatagccat tccaataact aagaggatgg cagcttggac taaagatgtg
     1621 gtcatacctc ctaatgtctt gcctatgaat acagtatccc tgcttacggg tgctaccatc
     1681 acactcttaa gaaaatcgaa ttttctatcc catattatat atgagccaaa aaacacagaa
     1741 gagaatatta cagacatgct aactatacca ggaaatatga atttttgata atctacacca
     1801 gtagaaggat gagtggaacc aaagccagag ccaataacaa acagccacaa tactggggtg
     1861 aaagtagatg ctattaatcg gcttcgttct ctaagaaata ctttgaactc acgaagccag
     1921 agggcatata atctgccaag gtttgcttct attatcatag tatcaactac tttgttgagc
     1981 ataatatttt ctacgaaacc tccttcacac tgttgtcctt ggttatatgt aacctgctat
     2041 aataattctt atgaagacga aaagagtata cctaatcatt gataccgtcg aggaaagaat
     2101 ttggaataaa tgcaaatcag agtctttctc ttcgagaaat ttttattagc agacttctag
     2161 gaagcaaaac cacacactta aatttaaaaa aagagcatct attttatttc tgttcgtagc
     2221 gtgcatattt ttccatgaaa cctccttctg cctcttcctt gatattccgt ccagtagatc
     2281 taagaaatac atcgttcaat gtggggattg tatattcaac cgattcggca tcaatatact
     2341 ttaaaattat aggaagattc cgactagcgt tatctactga taaaacaagc aatccatcat
     2401 cactgatatt ttcaatttta tgaacgaagc tgaaattctt taacatatct atattcggcc
     2461 gcttcttttc cactagcttg agttttacaa tatcacctcc catcatacct ttcaattttg
     2521 agggtgtatc aagcgcaatt attttacctc tatctataat accaattcta tcgcatagca
     2581 tatcagcctc ttccatataa tgagttgtca atattatggt gatcttctcc tcttttacaa
     2641 gccgctcaat atatttccac atagattccc tgctacttgg gtcaagacct aatgttggct
     2701 catctaggaa aattacttta ggtttgtgaa taagcccccg ggcaatttct aatcgtctac
     2761 gcattcctcc agaatatttc ttgacctgat cgctttttct ctcagtcagt cctactaaat
     2821 caagtacttc agcaattcgt ttttctctga cttgacgcgg cacgctgtaa agccaagaat
     2881 ggagtttcaa attttcgtat ccagtgagca tatcatcact acttggggct tggaacacaa
     2941 tgcctacgct gcctctcacc ttagcagatt gagttacgat gtcaaaacca tttaccgtgg
     3001 cagtaccaga agtaggtttg agtagagtcg caagcatatg tatggtcgtg gtcttgccag
     3061 caccatttga ccccaaaagt ccgaaaacct cgttttcatg tatatcaaga ttgagtctgt
     3121 cgacggcagt caaattgtcg tatcttttga cgagatcacg tatagctatc aaacaatcta
     3181 tcgagatgta gatcagggta ttatgccttg agattttaaa aaaatagaaa taatctattt
     3241 gaaaggtgcg ccagccgaca aaatatccat tcgacaccta acaatgataa taaaaattga
     3301 ttgataagat cacttgccta ttttgaacat ctttgtcccg cacacagtac aaatcccttg
     3361 tgtggctggc tttccatttt tcattgtgac ctttttctcg tctttcatct ctctttttga
     3421 cttacattta acgcaatacc cttccatggc aatgcaacct gttatgcaag aatataaaat
     3481 ggttgccata aaacctagcg agtcttatcc tgtgtaacaa taaattagaa tatttaaaaa
     3541 acttggaaat acgctaacaa tttattaaaa acacttactt cttttagaag actttgttaa
     3601 attatagttc tatatcatga taatcatacc tgttaagtat caacagcagg gaggaaaaat
     3661 tactctaaag ttcaatgtct cagaacaggg ttgatggctt ttggataggc catcgcagct
     3721 atcgcaaatg ttccttcact cggcgttttg tttgtatact tggtctaatt cgtcactttc
     3781 ttgttgctct cttacttctt gactaattat ttcacattcg gccttttctt cccgcttaga
     3841 agccatcaag ccttatcagt ccgatgcctg aaattacgcc aattattacg atggattgta
     3901 gggttgatat tattgtatgc gatgcggatt gagcagtcac atgttacact ctccataata
     3961 cgttggctat tttaacaaaa tcaggtattc catttaatag attaacctat tgtctcttta
     4021 gatcaccaag ttcagcatag cgaacgaggt gtacatttgc atacgtctgc tgaaccttta
     4081 ttatccgcag gatgcctgtc acaggcaaag agagaggtca cattcatcac aaatgtagca
     4141 aagtctatca aagtcggatg ataatattat gaaatcatga catttgggac attcaatctc
     4201 gtgttctata tctttcaatt tcttttctgt gacttaagtc atatcaacac ccgacacata
     4261 atttggattc tacggttgtg gcgacaattg actgctcatc ttagacatat tatgggtttg
     4321 gtcggaatta ttattatttg atgagtcaag gagctggatg tcgatgtttt agcttttgtt
     4381 aatgatttta ataattttga atcacttatt caatcaggaa tatgcctggg catgtttcta
     4441 tctaagagca aactcatatc ttctcatcca tgttctccca atgccagata ttgtcaattt
     4501 aatgctattt tcttcagaat cctccttttc ttctataaag cggttatatt ttagaaaacc
     4561 aatccacgtt gaaaccattt gctcattatt gtcaaatttt tgcgctatat cctttatcga
     4621 ttgcccatca gacaagcatt ttaaaatata gatcgcacca gtaagtccag agaagcgatt
     4681 aatgtttaca ggatggttag tgttgttctt tagcgtacga atatggacct ctttcgcacc
     4741 gttatgacgg tatgcaatcc tttgttcgat tacatagtct ttttccccta cttaaaggtt
     4801 ttacaggaga tgagatggcg ctgtcatagc taatcattga agttgccaaa ttatccctca
     4861 gtgtaacgga agaaagcatc attaagactc gaacacgcct tatattagtt gtaatattgt
     4921 tacttgaggt atataaaaat accgttgatt aaatatgttc gggattccaa ttaatttgat
     4981 attctcgcat gatataatta ttcagtctaa tgtcacatac agtatactgc cggttagtgg
     5041 aataccaagg ttcgacatta ctaaggctgc tctatggcca tcgctatact actgttaaat
     5101 gcatggatct gagttgtcgc atttgcaagt gttgcaacag cattctggaa cacctattga
     5161 agtttattcg aatttatggc gactatctac tggaattgga ggtagattat tccaacgaaa
     5221 aataacccaa tgactattgc tactattttt gctactttct ttaacacacc caattaatac
     5281 acctacaaag aaacctctac ctatggtaac actcattgat gttatgtttt caatcttcaa
     5341 taggtgccgt tcaaccctta tcattcaaat agcagtggta ttccattggt ttgtcgcaaa
     5401 agcaaagacc atgtcaacaa acaatctgac atctattgaa taatcagaca attcgataaa
     5461 gtggtattct caacacgatt atcagaggta atagtcaata ataaagaaca agagttcatt
     5521 aaatcaacag atatttttaa taatgtagaa gtatatacgc ctctccaact aacaaaatag
     5581 agataatttg aatttatgaa aacaagagaa agcacgaagt acatgattgc agctggcgtt
     5641 gttgcaacag ctatagtagc aggcgtgata ggttggagta agaggaaatc taaagaagtt
     5701 cgaacggaaa tagatatcag agcatctgat gatcgagtat ggcaaatact cacagacttc
     5761 ccaaactttg ttaactggaa tccgtttatt actaaaataa gcggagactt gaagaaacat
     5821 gcaaggctaa aagtccgcat tcaacctcaa ggcgaaagag ggatgacttt taggccaata
     5881 atacttaacg tggaaccaaa gcgagagctt cactggatag gtaggcttgc agtaccaggg
     5941 ttattcagcg gagagcacat tttcacaata gagcccctag ggaaggatca tgtacgtttt
     6001 atccataggg aaatcttcac tggtctattt gtgctaattt atgctcgcaa ctttgataca
     6061 aacatacgtc aggggttcaa agatatgaat caggctctaa aagctaaagc agaacaatca
     6121 atatctatat catgacgatc atacatacta taaattatgt ttcttagtca gtcaggttat
     6181 tattgttatc gtaattgctt gcaccactga ctaatgatca atatcgcaat tttatagact
     6241 cgctaccaag cccagtaaca aaaaaaatgt atgtatttac tttaacaaat atagacaata
     6301 cgttcgtgac aatatcataa gggatactac aaaactaata gaagggcatg ggtaggctgg
     6361 gtaatgcac
//
LOCUS       DAOR01000044            2711 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_113368, whole genome shotgun sequence.
ACCESSION   DAOR01000044 DAOR01000000
VERSION     DAOR01000044.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_113368"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          837 a          493 c          472 g          909 t
ORIGIN      
        1 gtcttcttgt attttcattt tttttctcca acttctctat tttcttctgt gtgaggtctc
       61 gcagttttat atgttcgtct atgagaattc aaatacacag aaaaagggag ggacttaatg
      121 tctaacaata ataaaagatt ggaaaagcta tgcaatgaca tattcgcact aaaccccaat
      181 atacgtttcg ctggtgtagt aaatattatg ggaacactca tagtaggagg aatgaggcag
      241 agtgtaaaat ctatggaaga taaagtagat tcttcaaaac tgtatttaga atctgcttat
      301 cgggttctaa agcacatgtg tctatgagta atagaatcgc aaacagacgg gactccatcc
      361 aaaagtgcat tggtgatttc gtcaattcta agttgcttcg cattgtaggt acaagaaagg
      421 ctgaaaagaa taatttcctt atagatgtgt atgaattcac gtgggcaggt ctttgcctat
      481 tcttattact acattataag gggatcgata aacctttcat ggaaatatgt gttaatgttg
      541 taggtgcatt tgcaacatat ctagcgatac aacgttcccg cattaaccgc ttcgcagccg
      601 atctttactc aaaatcagca ggaatagaag aatgtggtaa agccataatc gaccttttat
      661 caagaatgct gcatgatgac gacagtgcgg tactaaacca atacttgtcc gaatttttgc
      721 acggcattat atatctgttg cttacacaca gagagattac tccttctttt tttgagaatg
      781 ttttcaaaga ggccctcttc aaattggatg aagaaactag agcactaatt cttcaagata
      841 tgaagcttaa agtagaaaat gagataatac aaatgggggc ccctgaagac tacgaaagat
      901 tatggtttgc caatattcat atctacagca aacttgtttt atacggcgcc cgccatatat
      961 ttgccagaag agcaattctt atgattagat catattcaaa gtattacaaa gctccttctt
     1021 aaattgctgt acctgcaagg atgagttggt tgacttgatc agataagttt gcatagcgat
     1081 caataaatca ttgtaactgt tttttctcaa ttagccgtaa tcttgacgtt agaggtagga
     1141 tttgatctga aaaacgcgca atatcatcac ctttcattcc atcatgctgt ctcagtgatt
     1201 gatttagctg ttctaattct gtatgacaca gtgtgatttg acacacctta ctcatcgccg
     1261 tatattcttg cgctgctttc tagcctctaa aattgacctt atatctaaaa tgaacttatc
     1321 aaacttgttc acatgggcac tttcttgaat gttcttctca ctcatcacat agctaccatc
     1381 agtatcgatt tccactattg ccctgccttt tttagatggt ttcttaattg tcaattacag
     1441 cagtcatcat acttggtaat aaccaaaatg gtgataggtt tgacatttac atcatttttt
     1501 ccagtaagag tttggtgcag acaaagaata ttttatcttc ttcacagata attgcattat
     1561 gctacccacc tttccagttt tctggtcgat tagtgcgtat gcgcgatccc ctacgagtcc
     1621 gcgttccatt acgtacggag aatgagctcc tctcccaaca ttgacttcac aggatatcgc
     1681 catatggaaa ctactgttcc tgtagcagtc cgaggttgat tcacaaacag tcatcctcta
     1741 tgaaaccaag tgcacattct ccaccatgtt ttatgttaat gaatgaaccg ttaatgaata
     1801 aacctaaggc ttttatcccc tgtttccttt agaaaattat tgatatctgt tatgatattc
     1861 gaaaaactat tatcgaatgt cctagcatta ggggacaaac catatattct atatcttgtg
     1921 gttgaaaacg ggatcaccga taacaaccaa atatttttat aatttcggtg taataattcc
     1981 aattgtgata ttttatccaa atccacctta ttccttataa tttctataat aattgattta
     2041 tcaggcgttt tgtataagct ctgtagattc ggtataacta catcaagggt tagattatct
     2101 tttacctgga tttttctttc agatggaaga gtacagaccg taagcataaa atgaagaagt
     2161 gcttcactta agactgcaat tgtttcatcc ttattgccaa catttgattc aacatttatt
     2221 tttgtgatgc attcagccat tataagatca atatactttt ccgagaagtt tatgtttgaa
     2281 gtaatatcag atcgaatcct ttccttccct atgctatcta tcgtcgagta taaggtgtcc
     2341 tttacttcgt cactgcttaa catctattga atgaatgtgt taatcgaata tgtttataac
     2401 gattatcaaa gcacatgtta gattaattgt gtattccgtt tataataatc aatcgaacaa
     2461 agcaatgtag attaattaaa tacgaggcca tattaatttg cgatgatgca aacaaataga
     2521 gatagaattt gtagtttctt agctttagca attccggtaa ttcgtctatg agctttacta
     2581 atgtgtctgt attagaacaa attcgagcta gagtggctaa aacattaaca catggtattc
     2641 agctcaatat gtcaaccgat gaatctaaaa aattgctgaa gagacaggta aatagaaaaa
     2701 caaatgttgt a
//
LOCUS       DAOR01000045           11306 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_18089, whole genome shotgun sequence.
ACCESSION   DAOR01000045 DAOR01000000
VERSION     DAOR01000045.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11306)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_18089"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    7549..7636
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10821..11039
                     /estimated_length=219
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3406 a         2089 c         2018 g         3486 t
ORIGIN      
        1 agctggtaat atgtcaaacg ctgtaactaa gctcggccaa gcggttggtg gcggtcttaa
       61 gggcctcggt aatctgatat caccgggtaa aaagtaaaaa actcttgttg ctatattagt
      121 gtatagcaac aaacaattaa ttttttaatc tagccctaag ccgagaagtt ttcatccccc
      181 tccttcggtg aagatattac aacatcaact catcagaata ttgtttattg tctaccattt
      241 ttgattagct tgacttgtag cgaaagacaa ggcattcttt ccacattttt ttggtagctg
      301 gtgaaatcta attgctataa agtatttgcg tttaatatgt acgtgagtgc agaaaaaaaa
      361 gaatccacta aaacgtgatc aagcattaag tgatgcaacg aatggacaga tggggaaaga
      421 acttagagcc gtgcctgtac ctgaatcaag cgaagccatt atgcaatatg ccctgatggt
      481 tctagtccgg ttggtaatgg aacttgtgtt ggcccagaaa ttggaggatg tacctatggc
      541 gctgacgcga atggtaactg tcaaggtgta acttaccgtg gtggaccaac tgtcaatgga
      601 tttgtcctgt tgcaccaccg acaaacactt ccatgacact tccgccattg tgttgcgagg
      661 cagcatgggt atgtatattg ttatgacttt gggcgtggag cagggaatca aggcctgggc
      721 gattgccacc attggctaat gccgataaga cagaagccga taaatattgt tagggtttta
      781 agcagggaca aatagatcta gccaatcaca acagcaaaat catgtgatca agtgatggag
      841 agagaatgat tgtctgacgc tgaaccaaac gatatatcaa ttcgtttcga tgaggctacg
      901 ttagaattgc cgtgtgaaag acccccttag ttaatcttta atatcatacc gtttaagctg
      961 taaatataat gctatgtcgt atacaatttt taataatcct cccaatagga atggcgcaaa
     1021 caatagcgat acagattgca atattgaacc tgctattgat ggagatatag cttgcgtgat
     1081 attcctagag atgtttgtta ttccagcagc tgcagtcctt tcatcctcct taaccactgc
     1141 aacaatatat gattgtcttg taggaacatc catttgtgat agagccattc tggcgagata
     1201 gaacgctatg gctattggaa gagtaggaga aaatgcaaca agtattaata gaatgttaga
     1261 gggtaaatgg gtgaagacca ttgtattaat caaacctatt ctatctgcaa ttttagctga
     1321 tgcaagaaat gaaaatgcag taagaacccc cgcaatcgag aatatataag ataacaaagt
     1381 caaatctgct ccaaattttg taaaaaacca aagtgataca atgctttgta taacaaagcc
     1441 accaccgaaa gagtcaatag caaaaagacc cgataattta gcgacaattt tttttgatgt
     1501 tggtgacaga gtttgtgtta aaggttttgg tattcttttc tcatcttggt cttctcctcc
     1561 tccttctctt ccctgcatta gctctaccct tttggatagt gaaaggtaaa ttcccaacac
     1621 gcctatccct aaaatgctat atccaaggaa gagtagtcta atagagtcaa tttggttcaa
     1681 tccatattga tgttgaataa tttctggtaa gcccgaaact agaacgcctg ccgacatcgc
     1741 aaatgttccg accatattgt aaaaggcaaa gagtgtattt cttttctttg tatcctttac
     1801 tgtttgaggg agcacggctt gttctataga aaggaatgca cctgtttcag ttccagtaac
     1861 attaattgtt ccaattaagc cggctacaat taatgcaatg taattagatg tgacaaaaaa
     1921 tatgacgcct gaaatagcca tcattatagc atagataact agcattttcc ttctgcctat
     1981 tctatccgca taaaagcttg ccacgagagt aaatattacg ctatttatta atgtagaagt
     2041 aagtattaga ccaatcaaaa atccatcaaa tcctattaat tttagataaa ttgaaataac
     2101 aatacttaga aacccatacg caaatgttct aagtattcgt gcagccaata gcagccttcc
     2161 atcctttgaa atccattgga atgaatataa cactgatttc ccgtctccta gctactgata
     2221 ttgcagtacg ccctattaca caaaaaaatt atacttggta aagtcattcc tctactgcat
     2281 atctcttact agccagatcc atcagatttt ttgttaatat tattccaaat attgtcgcta
     2341 caatagcaaa gagaagcgat gaggcccttg ctgcaataac tgtatgtccc ttagctacat
     2401 tcgaggtaat tagcttcatc attcctaggt gaacttgaag tgtaaggcta ctgtttatgt
     2461 gaggccgata ctctgcaact gcgagtcctc cccatgaact attaatagta cttgaaagac
     2521 ctgcagttag atactgaaat ttagccggta gaattatact ccttaagtca gtaaacagtt
     2581 ttggtttgtg atcccgcatt accaatattc gaattgtcgg atgtgtaatt ttaaaattat
     2641 tctcaagaat agaatttatc tgtctcaaag cccatacgtg tagctcaaac ccttcaatac
     2701 acccttgagc tttgtttttc attatttaac ttcatgtgat atgctaacca acacaacgaa
     2761 gcgaggactt tatcgaacgt ctaagttgct aagacaaaaa ctgcgaggta attgagtatt
     2821 ctcagcgttt aatcatttaa taaaatgaat cgtactgttt gttgttgcat cttttgctat
     2881 tctcttcact gtcgtccagc ttcgagtggt agccgaaact ccaagtcttg tctcaataaa
     2941 gttgtttgga aagccaaatc ttccattttt cctgcgagct aaactgaatg cctcacggtt
     3001 tgtcatgaag atgacctcaa catccatgtc aggagagttg agcggaggtc tgattttcct
     3061 tgaaggttcc tctgatagga aagtgacgaa cagtttggta gtttgagact ttatgtcttt
     3121 gaatggattc aattctataa tctcctcgac ttgagacata tttcgcaaga atactttgac
     3181 atcaccctct agcactcatt gaattctcta tgaattcctt tgataatggc attcgaatct
     3241 atttctgaag tctcaaaaat gatattaccg ctgtttatgt agcttcttac attgttgaat
     3301 cctaaagata agaacatttg acgcaggtct tctactttca ccaagttgtg tccgcgcaag
     3361 agagctacat actgagtcaa ttaatggaat tcttcgagaa acgtaaccct taatcaatct
     3421 ttatagattt tttctgttac ccagcaagta gcaacggaaa aaatagcttg acaatagctg
     3481 accaagactc ggttagaaat gaagaatgtt acgaaagctt aagtcagatt tcagtaaaga
     3541 atcagttaaa ttttagatag aatctctttg tgaatggaca ataatatgac gagagcaggg
     3601 agaacagaat gttcatcatt ccagacaggc aaagccaaga caattgttaa gaatagatta
     3661 cagtttgttg taggaattac tgtttctttg ttcttcgtag cagccttggt cgcagctagc
     3721 ataattttac cattagcata cgcacagcag attagatcca tcaacaaccc tgctacaaat
     3781 cctataaacc acatagtagt aattatgcaa gaaaatcgaa gttttgataa ttattttggt
     3841 acatatcctg gtgctaatgg gatatctaaa gggacatgcg tacccttatc tcctgacaat
     3901 ccaagtgtag gttgtgtaaa accatttctt tctactgatg ttatttcagg agatttaccc
     3961 catggttatc aatcatcgtg gttggatacg ataatggtaa gatggatggt tttatggtag
     4021 gcgaaaatga aaatcctaaa actatgacct attatgataa taaaacaata ccatactatt
     4081 gggatctagc aaaaaactat gtcttagctg ataactttta ctcttctgtt ctatagtttg
     4141 ccaaaccatt ggtatgctct tgctggtcaa gcaccagcta catcaatgtt ttattttatg
     4201 catagaccac caaataatga catcttaaac cagcaagaaa atgctagtac gattgcaggt
     4261 ggtaatccaa atgctgcagc aaattttggg gttaatccca atccaacatc caccaattta
     4321 agggatgaag tagcaagagt atatctcgaa gagtccaatc taaccaagac tgttgcagat
     4381 ctatttatga atgataccta caatattaca tggaaatatt acgaccattt gattcgagca
     4441 ggtaactata aagctgcggt aagtagtgga cgtgcatttg agttctggaa tccattttct
     4501 gcaaaaggca gtacgtatac tccagcatac gctccacact ttgtaaacag agcacaaata
     4561 tttactgatc taaagagtgg cgactttcct caagtttcat gggttactcc aagctttcct
     4621 attagtgaac accctccagc aaatatcaca tctggaatga actgggtaaa agacctaata
     4681 aatgccataa tgagcagtcc ttattggaat agtactgcaa taatattaac atgggatgat
     4741 tatggtggat tctatgacca tgtgccccca cctcaaatag ataaatatgg tctaggctta
     4801 gaatgccaac tcttataatt tcaccgtatg ctaaaccagg atatattgac catacgcagt
     4861 atcagttcga gtctatgcta aagttcatcg agtggcgctt tagccttcaa cctctaactg
     4921 aaagagatct tcatgcgaac aatcttttaa acgcattcga ctttagccag aagcctcttc
     4981 caccacatgt agtcccattg acaggagcgg agttaagcgc aattcgtcca tacataaatc
     5041 tcgtaaggac tatcgattaa ctttaccagc aggtagacca aaactatgga tcgggagaac
     5101 aaagtttggt tttaaaccta agaaaccaag cctttagtta ctttgataat cattaaggtt
     5161 aggacaaaaa cgttactgtt ttctaaaatt ataggataac tacgtctgtc tggctaatag
     5221 atacagttaa ctctgttaat ccgaatatca attatattat ctgattacga tggactctcc
     5281 agcagataaa tgaaaaacta gtattataat aacagcctat cctatggaat tgcaaccgag
     5341 tcagcccatt agatttaagc tttccaatat ctgtgcggat acgagaaaaa caaacactcg
     5401 atagttagaa ttcaattctt ctagtctaat aagctctaca agcatattta tgtaaagtgt
     5461 gtttctatgg attgtactcg tatagaatgg atacacagca ctcggatatt atatgaagga
     5521 tcttaatgtt tctattttat taaatcatcc taattcatgc aattattaat aataatctca
     5581 agtattttca ttcatgttta aatattaagg cactgtttta ttagaaaatg atcctgtggc
     5641 tggtttggta gtattttggg ttgaaagatt ttttacaaca cctccaaatg attgttttgc
     5701 ttggtcggta atatttttca caatcgatgt aagaccaact ttatccgcac ccttcttaac
     5761 acctgcaaag aatgtttgcc atcctagccc cattatagcc aaaattacga cagcgattac
     5821 tatccatgta attaccccca tagaactaac cccgatgaag gcatcgctag taaatgaaca
     5881 tatgcctatt ttaacggtta tggagacact cactagtttg attatcacga aagaaattta
     5941 cggcagggtt gatctcttat ctttatccat cggtgtatac ttaagatgac caatataata
     6001 aaaaaacttt tcgactacca gacgtaagct ggatgagctt tttaagaggt aaaaggagaa
     6061 ggcggcatga atttaatctc cttgctattt atgcattcat aattatacgc tactgacagt
     6121 attttttctt ctctaaacgg aggaccaatc atttgtactg ctactggcat attatcttta
     6181 gttaatccta tgggaatact aactgcagga aaaccagtgc tattaaatac tatagtattc
     6241 ctaagtaaag cttctctagt ttgcaaaaca gtaccgcaaa tttcagagac cgctaattca
     6301 tcgaatctag gagcgggaat gatagttgtt ggaacagcga taatctcaat tttgtgtctt
     6361 aacatggcta gaatctcctt ttttaattcc tcatttatga ttctcatggc atgtatgtaa
     6421 tcaaccgcag atatttttgt tccttgaatt agcatctctc ttacttcttc gctataatct
     6481 tcctttcttg tctttaacca atgcgcatgt atttctgatg cctccgcata ccttattttt
     6541 ctccaagtgt tgtagtatct ttcagtattt tgaagatcta gatcaaatac tttataccct
     6601 aatgacttta tactttccaa aaactgataa aacagacttt caacttccgg atgcacataa
     6661 tcaaagaaat actccttcgg tactccaata gatatacctc caaaatcgga tgtttcaata
     6721 attttcgtat agggaggcac ttttttgtga ttggaaatgt aatctgacga atcccaccca
     6781 gatatacatt ctaatacagc agctgcatcc catgcgctcc tagtaacgca cccaacatga
     6841 tccaaagacg gtgccagagg cacaacgcca tacttactaa cccttccgta tgtaggtttt
     6901 agtccaacta ccccgcagag tgatgatgga actctgatgg accctccagt atcggttcct
     6961 aaagatacac aagccatccc agttgaaact gcaacagcag aaccgccact tgaaccacct
     7021 gatattcttg acatattcca agggtttttc gtgttaccgt aaaagggatt cactccggta
     7081 attccagatg caaattcatt caaattgttg gtaccgagta aaattgcacc agcttcctta
     7141 attttcttaa tacaagttgc atcagttttt ggaatatatt ccgacaatat tcttgagcct
     7201 ccggtgcatc taatatcttt tgcataaata atatctttta ttgaaaatgg aatgccatgc
     7261 aaaggccctc gatagtttcc ttgactaatc tccctttcag ctattcgtgc attgttatat
     7321 gcatcttcct catcgataat agttataaat gcgttaagtg atggattgaa tttcctaatt
     7381 ctatcaaagc aaaactcgat cagatctaca ggtgatattt cattggtctt tattttctct
     7441 gacaaatgtt taacagagaa taaagaaaat tgtaatcctt ttaccatagt tgttgtggtg
     7501 ttctcgtgat tggacttttt aaatttttac gctgggtcac tatgatttnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnngtca ctatgatttg aatagacata gtacgtggtt acacgatatg
     7681 tcaggctaaa agaaattcaa gtatttcgct ggtactttac tcaaataaaa aaatactgga
     7741 gatttttggt actcagatga tatcaatatc cacttgaact actcgagatt cctgagctct
     7801 gtgagctatg atgtgagtga ccgatagatg atccatgatg gtgagtatgg ctagggctac
     7861 tactggtact attactgcta ttagtcgagt tggtaatact acttattgag ttgttcgtat
     7921 tgacttgacc gtgatgatga tggtgggtgt tgatcttagc agcgaaagtg tttgacgtca
     7981 gaagcgttga cgttgtcatt cctactgcca aaatggcgat tatagacaat gttctcgcag
     8041 ttatattcat tctaccatca tacgagagaa atccttatac gagattagtt taacttcatc
     8101 ataactaagt ttgtggcaat tttatcttag ctatgatgaa tagacagcga cctaaaggac
     8161 tacaaaccag gggtgttgac attctgttca attaggactt atccgagaga ctaggagcaa
     8221 aattctgaat tcgtcaaggt catgaattct cattctggat tactgcatct gcagcttcat
     8281 cctgatcttt ttcgtgttga aatggaaatc taatttttga gattgcaacc acgctaaata
     8341 tagatacatt gaatgtacaa ctaaaacctt tttggcatgc tgctagtctt tttcacgcaa
     8401 aatgattctc caggttcaaa acgtaaacgg ttcaaactat taaaattacg cagccatgaa
     8461 taggtttttg ttgtctcgaa ctttatatat tccctgcgtc ttagttgcta caatcattgg
     8521 gtatttgagc tttgacagga cgtcatacga catggtgata tgtatcgaaa cacaggtaaa
     8581 aaaaagtgta tactcattat agtaaaatag tcacttctaa ccaaagatac ttagaatcaa
     8641 taaaacagct acatagtttt atttgaggga ggctaagata aagtttagta ctaatttggg
     8701 cttctcataa atagttagta ttcatttcct atctcggaca acacatgaaa tgcagaagta
     8761 gaagcgacat agtgggatta attctaaatg cagcaaatgg tggcggagct actaaaacaa
     8821 agttaatgta tagagcatat ctgtcttctg ctcaactaag agaatacctg ggactattag
     8881 tggaaaatgg tttgatagaa tatgagggac atcaaaccta tagaacaaca gagaaaggta
     8941 tacgtctatt gcagatacac gacaaaatgg aagaactagc tccattatgc ttcgcagaaa
     9001 aagcatagta aacaagataa tagattatca cggaacagga tccgccgtta ttatagtcga
     9061 acaatatttg agaagtagca atagcattaa gtgactgaaa acgaacaaag gggaaaaatc
     9121 ataacagaat atcgaactcc ggataaaagg gatactgact tggtatttgc agtttatcaa
     9181 gataaaaaca caccaaatcc tgattccttg tatgatgatt tgagtaaatc atttgcgaat
     9241 acattagatc gttcgggaaa aggtgatagg gaagatggta atcaaaggcg tcgacaaata
     9301 actcttcact cttttagacg attcgtgaaa accacaatat cagatcttgg atatgccgac
     9361 ttttctgaat ggtttatagg acattctggt tctacatatt ggacaaagaa agattcagag
     9421 aaagcagaaa tatttcataa gattgaacct tacttgacat ttctaaatat accacagttg
     9481 gaaagacaag gtgcagatat acaaacgaag gtcgaagaac tagaagaatt aaatcaatca
     9541 ttaagaaatc gtgacaagct gaaagatgat gccatatcgc aattatcgga tcagcttatc
     9601 gcattaacaa ctagattaga ttcaatcgaa agacgacagc gataggagat cccttctttc
     9661 aatgaagttt agccagactg aagtatccaa agaaggcaag gttgttcatt gttaggtggt
     9721 ggcttattag tccattcggc attactttca taacgtcgga ccataacatc ttaaattgta
     9781 cttacctggc cgacaaatat aaaaaaagtg gaagtatcat gaggtcttta tgagcttgcg
     9841 gaaatgacca gcattctttg caatctcttc atcctaagtt tcttctgtat atccgtgtac
     9901 ttcaattccg tgtttctttg cgtttgtaat aattcttcct cagttttacc atacataata
     9961 tgggtacata ccgggcaccg gcatctttgc agtttaggct taaggtcata gtattcctat
    10021 gctatgacct gatgatctct ctttcatgta taaggctttt actctcttta gtggaactct
    10081 ttacatgagc aatcttcata gagtctaaag tgccggttag ctgcaaaagt ttatgttcgg
    10141 ctgtaggata aaaacacaaa agcccctact ttttatttta ctttcgacaa gtagttagct
    10201 gagaagcaag ggttaatatt tccactctcc agatatatta ttccaccata aacggtatgg
    10261 tttagcatcc tttccaattt cttcttttat tctaaactca atcgaataaa acatattctc
    10321 catagcgtca gttcctccat cggcgtacag ttctcttcca atttcctcta ttctctgttt
    10381 ttttgattca aagtctgagg catcaggatt ttgtgcacaa tgagtaagaa gatcgtaaag
    10441 ttcttcttcc aaatacgcat ccattttctt attactttat tttattgctt ctgagataga
    10501 atataaatta tgcagcatca gtaatgccaa tattattgtc caaatatttt cattgtactt
    10561 tctccttgtt ttgtgaggat aacgttactt tgtctgcctt tgtattctac ctcgaccaac
    10621 ggattgtcta accctccagc aatgcttata ggatttagta aacccttaag tctgctgtgt
    10681 ttggcctcgc ctgataggtt tttgtctacc ggtcctgctt cttctagctg ttctagaagt
    10741 ttgtttttct tcatcatctt ttgtgatccc ttaacgttgc ttaatattct caggacagca
    10801 agagattcgt ctttgggttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    11041 gattcgtctt tgggtttgtt gattggagcg gtagagactt tatcaccgta tcgtagagga
    11101 tttcttgtaa aaagatcaat tatctttctg tctgctgcta cgacaatcca gcagcgagca
    11161 cgaattatta ttgtggtgtt gagggatcca ttgactccat tatctttgca gctgatacta
    11221 gcaaattctg tgcatcttgt tttgtctcat gacgtatcag attaaaacac tggtttctaa
    11281 attatagtct taattatgtt gttcta
//
LOCUS       DAOR01000046            2891 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_81128, whole genome shotgun sequence.
ACCESSION   DAOR01000046 DAOR01000000
VERSION     DAOR01000046.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_81128"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    284..509
                     /estimated_length=226
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a          531 c          458 g          802 t
ORIGIN      
        1 atctccaaca tcaaacaaga atctcggaac aattctaatg ccgaattcat ctcaatacct
       61 tggtaatata ggaacaaatt ctcctctgtc attcaacgtt ccgctgcagg cagtacatgt
      121 gacaatatcg aaaggtcagc aacagaacaa tattaatact aacaaaaata tcaaagaaaa
      181 gataccatcg ctaactagaa ttgcattgaa ttcatctgct atgcaatcca atggtgtggg
      241 aacagacaac aatataactc ctcggattta tcctgtttca ttcnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atttatcctg tttcattcaa gattacatat
      541 agcgataatt tgaagaatat tcatgaaata gttgtaaata gtcctcttca gattaaacca
      601 caacaatcta aagtatctcc tagtcaaggt gacggcttat tgggatttct aatcggtgct
      661 gttgctattg ctattggcat cgccgtggta tttatcagaa aatggctaaa gtctggcaga
      721 aatgagaatc ttgtttcgaa aaaatacaag aagatagcga gcagaatgac gaaaacgagt
      781 gcaggagata gcgaaggcgt gatacataac gctcatcctc cagaagaaaa gaaagcagga
      841 gcccccattt cactgatgta gtattcgtaa aagtaaagta aattatcgcc ttagaaaagt
      901 tgaaccccga atccggaatg gatcagcatt attaaaggat aggaaaccgg aataagaaga
      961 aaattacttc ttgtacttga tatatcatga aaagaataga agcaaacaga ataacggatt
     1021 atcataggat gctctgaaaa agagattacc aacacttgca atctgtcaca aagccgagca
     1081 gcacatgttt caggatatga gttctcttca tatgtcctgg tacatcatgt gctcaagatg
     1141 gctgcgaacg tgtgaaaatt ccggaatcaa attatgctca agctcgtata tgtttgataa
     1201 ataaccccac agataaccct agcaattatt catcaaaaac caaatataga atagctacaa
     1261 aactcagtta tgatgaagac aaactaattt cctataaagg atagcctcta ctagtccgag
     1321 tacatgtacg taacaacaac acccactata tttgtcatcg cggtagtaat ggcaacagca
     1381 ctaacgctac tgacattgac tactttcaca tcgtctgtat acgccactag cggccacacg
     1441 catcatcatc accacagctc gaacttaaag tgcacaccga ccctgattca cttgactgat
     1501 cagtctgtaa caagtattca atattcatgc aatgtaccaa aaagccatac tactaccgtc
     1561 catttaccga actcgttagc cattggataa tcagcatatt tacaaacaaa atagcgcaga
     1621 cagtttgtag gtaaatgtaa atgtatatga acacgctaga acagatggaa ccaacaaggg
     1681 ttgcaaattc atctgtactc attaagctga tatctctaag gaataagact ttgtccattg
     1741 aattagcgta taccctagag ggattctcga ctctaagcgt cacggaaaat catttatgtg
     1801 tttatgattt ttcatatttt ccttacaagt caccaacttg gagatatctc tagcgttttc
     1861 gttcagcatg aattcctatt attataaaaa caagaccttc ttagaagtac tgtagacaag
     1921 aataccatgc atagacgtat agttgctttc agtacagcta gaattaaaat aatttataaa
     1981 tattgatttt ttctaattcc actacggcta gatccacttt actattttgt cttgttttat
     2041 taaagctcat caaatagctc agaatatcca tcggtaacct tgtctgtaaa gctttccctg
     2101 tatttgtaca ctacatctga caaaatcttg ccgttcacaa ctttatacaa ctggaactcc
     2161 tgtccatgat taactgacac tattccagat tcacaaagtt ctcttgagat gccaagataa
     2221 ggtagatggg gctttatcga tattttttaa gatttcgctg aaggtacaga gatcattctc
     2281 aagtataaaa agcactattt gtcttgcaac tttatttctg atgtattcta gtatatgaga
     2341 ttcatctgct gggatgtcga taggataata tctggctctt ctcccgtctc ttctgtggac
     2401 tgttatttta tgtgtcttct ctaatatttt tagatgatat tcaattaatc tgaggagctc
     2461 tctgtatctt atgcctggag ttttacctac gtgcactaga attagggcct ttgtatcatc
     2521 tgttcccgca acactaaaaa tatgtctaaa agtctacatg ctttgcatta aaaaatcatg
     2581 gtagctattt tgctttttgt ggtacattcg tcgaatgtat cggattattt ttataaaata
     2641 tctgtataac aataatgtaa ctgggaacta cttcattctc agaactcgcc gccacagaaa
     2701 atatcagaaa ccccgtttgc accatccggc ttatgacgtg gaactgacgc tgcttatcta
     2761 tagtaggtgt cacttgttgc tcatatccaa ttacaagttc tctatgactc ctgcagaatg
     2821 tgtgtgcact aggcgaattg tgaatcgatg aataaccctt ataagaccat atacataggt
     2881 gattgcaatg a
//
LOCUS       DAOR01000047            3916 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_84653, whole genome shotgun sequence.
ACCESSION   DAOR01000047 DAOR01000000
VERSION     DAOR01000047.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_84653"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1261 a          741 c          756 g         1158 t
ORIGIN      
        1 agactcccaa gaaatatttt cggacgaatg attccgaaat tcattgaata gattccccct
       61 aagttcatta gaattaatct tcgcaacctc tactctggag tagtctttag tatcggttct
      121 gaaattcaga ttcacagcct gtttgaacat taataactag ttgtacatga attcggaaat
      181 ccatatttaa tgtatcatta tccatagcat atatacatac taattattaa tagagcggac
      241 tctgagtgag tgaaaaactg ttgactacag caagtatatg gggtgataga ttcataatag
      301 cagcaataat tcaggtcgca attattaccg gtcttacatt attaattgtt gcaatttcag
      361 ttactatttt cttccgcagt taatataatt caattccact ccttatcttt tgaaggtcaa
      421 gctaaatggg ccttggcttt cttggctaca tattctatat aatattagtt gtccaatagc
      481 agtgacagcc gtcttttata accatctgga gatcaatgtg gggaaaaaaa tcagaggatt
      541 gaaggccgtg ctagcttgaa ttcatctgag tggaatgaat gtaggtggta ctgctataac
      601 actaaccatg gatataggat taattggttc tgaatatgta gtttaattgc caatagaggg
      661 agttcttcgg ctgttgcgag attgacttca aatcctgcgg tcatggtaca atttattccc
      721 cctgtagctg catttgcagg attacgtagc ctaggagtca tggggcagat tgtataccat
      781 acatagcaat ttatttgcaa aaaaattaat cctaagttat atagatctca atcttacaac
      841 tcattgttaa tgtaagacgc gaaactctag ctgttcatat caagttctga aaaaacagta
      901 ccaagaatgg taataataga caagtcttcc aattagcagc tattcagtat cttatagtga
      961 tttatgacca tttagcgtta caacattata ttgtatataa tgagatgtga tttgaaagac
     1021 ctcaattaac ccaatataac gagacacatg gaatatggtt catgaaacca aaaaaatgga
     1081 gtgtctatat taccagaagg atattggacc ccgcaatacc tatgatatcg aaagagtgca
     1141 gcgttattgt tgcaaatcac aggggaaggc cacctactag aaatgagctt gtgaaagccg
     1201 tgcgtgacaa agatgcaatc ctctgcacat tatcagatat aatagacgca aaagtcatgg
     1261 atgctgcggg atcaaacctc aaagttatta gctcttatag cacaggcaca gatcatatag
     1321 atattaaaga ggcaactaag agaggaattt atgttacttt taccggtgat attcttacag
     1381 aagcgacagc agatctggca ttcgcattga tcctcgcaac atctagacaa attacgcaag
     1441 gacatgagct tgttacacgt aaacgatgga aatatggctg ggacccgcat cttctattag
     1501 gttcagatgt tcacggtatg acaattggaa tagttggctt aggtagaatc ggatcagctg
     1561 tagcacgtag agcaaaaggg ttcgatatgg atatcattta tcataatctc catagtcgaa
     1621 atattcagat ggaagagcaa tttggtgcta aacatgtcga tatgaaccaa cttgtagaag
     1681 aaagcgattt tctttcactc cattgttctt tgaatagtga aagttatcat ttaattaatg
     1741 aatcaaatct tcgacgcatg aaaaatgatt cgtatataat taatacagca cgaggtcaac
     1801 tcattaacga ggcacatctg attaaagctc tgaagcaaag gtggattgca ggagcaggac
     1861 tcgatgtctt tgaaaaagaa ccaccagcac caaataatcc attattgaag atgaagaatg
     1921 ttgtcttatt acctcatata ggcagtgcta cgtttttgac tcgaacaaaa atggcagaag
     1981 tagcagccaa aaatttacta aatgtcttga atcgaaaaac cgcaatttac cttgcaaatc
     2041 cagaggtaaa tcgtagataa atcagattat aaacttttta atccaagttg ggcataagag
     2101 actctagaac cctagagatt ttcgctttat tcttggaagt cctttcaaag acgatatatc
     2161 tatagggttc aaattatata aaaatagcgg ttataatcta aatagatcga cttgaattag
     2221 aaattcaaac agatatattt tcctaattaa aaattgcatg tacagataat gatccaggtc
     2281 attaagagca agacgcattt attcagccta taaatatggc aagtgttagc cattgatcta
     2341 tctgataaaa ccggtatctg cggggaattc aataaggagt ttgcaacgct gatggtcttt
     2401 ttttgtgaag atgtgttcta ctgttccaat ttggtgcttt cctttgtgta tagtttaata
     2461 actaagatat tattatcgat atttctaatg actacgagga taggaataag catatttttg
     2521 gcaatcatag tcgttacacc tattttgttg gccggcggtt catctgcatc cacagtagcg
     2581 gcagctactg gaacagtcac caattcaaca acaggtgtaa acccagcaaa aacaacaagt
     2641 acaacaacta caacaactac aacaactaca acaccgatca aacatctagt ggttatctta
     2701 caagagaatg ttgcctttga ccattacttt ggtacttatc cgaatgccac aaatctgcct
     2761 agtgaaccca aatttgtagc taatccgcat acaccttcta ttaacggttt gaattcggct
     2821 ggtctattaa ccaacaataa tacaaactct gctcatcctt tccgtcttga tcgttcacag
     2881 gtaataacgt gtgataataa tcacgcctat gcagctgaac agaaggcata tgatggtgga
     2941 ttattggata agtttgtaca atatgctggt cctaaatacg gtcactgtga tccaaaccaa
     3001 gtaatgggtt actttgatgg caataccgtc actgcactat ggaattatgc tcaacatttt
     3061 gctattagtg ataactttta tggttctaat tttgggccct ctacgccagg acacatcaac
     3121 cttatttccg gccagactat tggtgcttta ccggctgata cgaaaattcc tccatcttta
     3181 aaatttgatg gagttgtgaa tggcactctt ataggcgaca tagatccaaa attcgatgat
     3241 tgttctagga attcaaaatt atataatgtt acagttgcga tgcagggcaa aaacataggt
     3301 gatcttatga acgcaaaagg tgttacttgg ggttggtttt caggtggctt cagacttctg
     3361 agcaaaactg gaaatgcaac attagattgt aatagccgtc agagtcacac gagcactagt
     3421 gaaataccaa acaaggatta ctatcctgat gttgaaccat ttcaatatta caattctact
     3481 gccaatccac atcatcttcc tccaacctcg gtagagatgg tgggtcacac agatcaagcc
     3541 aatcatcagt acgatctaac acacttctgg aatgcgtctg aaggtggtaa tatgccttca
     3601 gtcagcttta tcaaagctcc cacataccag gacggccatc cagaaatctc cgacccacta
     3661 gaagaacaga ctttcttggt taatacagtc aatcgtcttg aaagcttacc agaatggaac
     3721 agtactgcag taataatcac atgggatgat tcggatggtt ggtatgatca tgtcatgcct
     3781 ccaatagtca gccaatctga cgatccaata aatgatgctc tactcggacg tcaaggcctc
     3841 tgcggtcatg ctcctaccgg tgctgcgcaa gatcgctgcg gatatggtcc gcgttagctt
     3901 gtagcacaat agatat
//
LOCUS       DAOR01000048            3412 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_130318, whole genome shotgun sequence.
ACCESSION   DAOR01000048 DAOR01000000
VERSION     DAOR01000048.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_130318"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1181 a          610 c          654 g          967 t
ORIGIN      
        1 cctactaaac aaagaatgtc ttactatctc tatgacagca ccaagcttat tttggacaaa
       61 tcttgggttt ttgagtgaaa tccctgtggc ccaaacatga atagccacga caacaagaac
      121 aaaaagagca aatataccaa ttgctatacc aagactagtt accttaccta agaatatatt
      181 tcccatatta tctggaaaat gcgttatgac aaccatggat atatggataa taataaacag
      241 aacaaaggca accattccta gaaaatgcag actgcgtgca acttggcgcc ctctgaagat
      301 cctaggatac cgtggaaatc gagcaccaac agctggagac attgctgcac ctgttgcaat
      361 catgaatggc ccaagtagga acactacacc aaagtatgca agttgttgta atggatcata
      421 agggttaccc ggaactgcaa agtgaaagct tgcatacaac attgctgtat ggaaagcctg
      481 cgggaaaata gaccatgacg ttgggattag gcgagcccac tcattactcg taaaaagtag
      541 aatataatat gctgctccat tagctattca aaaaataata gaaaagaagt gcaagtgtct
      601 tcccattcca agatttttat gtcctggtaa agcaacccat gaggaaaatg attcttcctc
      661 ttcgagcgag atccagacct tttcttcaaa aatcagacgg aaaccgtttc ttagtaaact
      721 ttatccattc tgtaccgggt gttgcatggt catgccaata gaattttgga agagcgctca
      781 atatttctat accacttcgg agtaacaagg taatgaaaat cagattgata aaatgtgcaa
      841 agcgaatcca aattgggaag tcataatata ccaactgaga attctctcct ctattgttac
      901 aagagttcat gaataattat ttaaccattt atggggttgt tataaggtag aatgcgttgc
      961 aaacatttgc aatctaaaga tctaaatact gaaatatctt agattgttcc aagcagagaa
     1021 attattttgt attgatatac atgcatgacc tgactatttt tacaaatcga acaaatcttg
     1081 ttatactgct cttcctcgca atagctttag cagtaataga ttcatcactc gtttactcta
     1141 aaatcgcata tgcggcattt tacaagctga gaagcatacc ttcaatatgc cataaccgga
     1201 aaccctgcaa tgaagacatc aagatcaatt ggttcaccaa caccatgtag agtagtaggt
     1261 agtaaataat aatagtagtg acaaaacaaa caacagaaca ggagcgaacc atctgatcat
     1321 atgtcaggag ttgatataga aaaggctaac actggtcatt cttctaaaca tatagtaatt
     1381 ttaggtggtg gctttgcagg atccgaagtt ttaaaaaatc ttcagaagaa acttagctat
     1441 aagaatgttg aaattacctt agtaagtaga gataactttc ttctctttac tccaatgctc
     1501 cctgaagtag catctggcat gatagagaca agacacatag taactcctct tagaagcttt
     1561 tgtaagaaag ccaaactcta tgaggctagt gtagaatcaa tagatctaga taataaactg
     1621 gttattataa cacatactat tggcaggcaa tctcaaccaa gcgactggcg tgaacataca
     1681 ctcaaatacg attaccttgt cattgcgttg ggaagcgaga ataacttctt tggcatgtct
     1741 gaaatagaag aaaatgcgtt caccatgaag actatcgatg atgccattat tctgagaaat
     1801 catatgataa acatcctaga acaagcaaat ttggaacaag ataaccatga attgagaaaa
     1861 agcttgttga cttttgtagt agttggaggg gggtttagcg gtgttgaaac agtgggagct
     1921 gtcaatgatt ttgtccgcga gtccatcaag caatattatc cgaacatcta catgtctgat
     1981 gtaagagtca ttcttgttag tgcgactgac aaaatcctag aacagataga tgaaggatta
     2041 ggtaaatttg cgttggaaaa actaaaagaa ggtggtgtag aatttataat gaacacacaa
     2101 gtcaagggag caacaaaaaa taaagcaata ttgcatgatg atactgttat accatgctat
     2161 tccctgattt ggactgctgg cgtaactcca aacaagttaa ttgcaaacct taaatgtgaa
     2221 cacgacaagg gccatagaat catagctaac ggctatttag aagtaccagc acatgatgga
     2281 gtttatgcac tcggagattg tgcatttatt acagatcctc atacaggaaa accttatcct
     2341 ccaacagcac agcatgctat aagacagggt aaggtagcag caatgaacat agtttctgct
     2401 atcaagggaa aaagagaagg aaagaaaaag aaattcgatt ataaaacaaa gggaatgatg
     2461 gcggagatcg ggaaaagaac aggtgttgct atattatttg gtaggattag attacatgga
     2521 tttcttgcat ggtggctttg gcgctcatat tatttagcta atcttccaac agcaaaaaag
     2581 aagttaaaag ttatgggtga ttggacttca gatttgctct tcaaaccaga tgtaagccaa
     2641 gtatcataga taatgtgaag gtttgcaaat cttatgtcgc aaccgtgtgt ttctgtatat
     2701 tcatttccta ttgggaagat ctgttcgatt cttttctgct gcatttccga atctcttcta
     2761 cacttttcag ctgctacaat aaggagattg ataaaacaag gaaagaaaga tgctgaaaca
     2821 gctcttacac aacaaaaaaa agaaattaga aaactaaggc tgatatgaca acaactagga
     2881 aatagtgtag aaatcctagt tgattagatt acggttcttg ctttggtaag aaattatctc
     2941 tgagcgatct gaaaataggg gagaaagatc agatcaggtc acattgaatc cataaattcg
     3001 ataaaatcag agttatgaac atgttttgtc ttttcccaaa ctttcctaaa ttttggcatg
     3061 tcaataagtg tttctgctaa tgccttccag cgaggccaaa gttttgtatc catcattttg
     3121 gttttataaa gatagaacag gaattcaatt aaatcaagga atgcaactac agtgtaatat
     3181 tcatcaggac tatcaactaa agccaaacgt tctgcaaata actcgttttc ttttgctatt
     3241 cttgtaaagg cttcagtatt ttcaagtttc atccttgtct ctattactcg agcatatatc
     3301 tgctgttcac tttgtatacg catacttcgc tttactgagg aaaactgcaa caacgacatg
     3361 ccaaacaatg ctgcaagtat tccaatagta attattggac taatttctga aa
//
LOCUS       DAOR01000049            3125 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_107204, whole genome shotgun sequence.
ACCESSION   DAOR01000049 DAOR01000000
VERSION     DAOR01000049.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_107204"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2689..2836
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          905 a          582 c          500 g          990 t
ORIGIN      
        1 agtaattcca ttatcaagta cgtgatagca aagagtagtg gaggacatca tcatcaaaat
       61 aaagattcct gctctgctat gtactctgag cattcctact gtcaaaacca gaacttatca
      121 tagacggtgt tgtcattcat tcattcataa tgtgacaaaa atacgacagg gtagataata
      181 tactttctaa aatggaaagt aaatagtatt atttttggta cttcctctat atcgcgtaca
      241 tacatacatc aggtccgcgc cagtaaaagc taacgtcgat acagaaccca atgccaaccg
      301 tgcaatagat tttaatattt ccatacattc attgtatagg agtagttact tgaataactc
      361 cgctcaatat tacgtcaaaa caaatcataa actgttatta taaattcaaa ctgtctctta
      421 atatgataga aataggaagc tccagatact ataattctga tatgtctaac aaacaaatct
      481 cggaggattt catactgtgt gatatatgta gtcaaatact agaattagac cattctatca
      541 ggttcgcagg ttttgccaac aatatgggca agataattgc agctcaatat agaaatagat
      601 tggatccatt attgacagta gatgaatcag tactatcttt tatagaatct gtgtttagaa
      661 tgagtactag aaaggacatg agatcaaaac ttggtaaacc tatctattca tgtacactat
      721 atgaaaaggt aaaacgagca acgatattat tagataataa agaccatccc attctcatgg
      781 tgtcgttcga cagcgacatt tcaggtattg atcatgattc tataattatg aatggaatat
      841 tgccattagc tacattcttc ttatcatcca gcgaagccat ttcgcataac aggtaagagt
      901 aatcgtagaa gaagcaaggc aagctagaga taggttcact tactatgcga gctatctagg
      961 tgagaaggaa acataaagta cggggtaagt aagataccat acaacttatc tgaagcgtat
     1021 aaactacaac aattgtacca gacacgctag aaagttgtcg ttcatcacat aattatttat
     1081 catgaggagc tgttgtcatg ctcgtctacc ttgtttagag ctttttttct ttttgtactc
     1141 gttccaatct ctcgtgtgcc taaagatatt atctcaatca gtttggcttt cttatctgag
     1201 tctggtttag gtctaagaag tcttcccagt ctctgaataa attctctact gctcccggtt
     1261 ccgctgacaa ttatacccaa ttctgcatct ggaacgtcaa cgccttcatc taacactttg
     1321 gatgttacta atgcattgta tcttccttct ctgaaaccgt ttaaggcatc ttgcctctct
     1381 cccttgctgg atttatgagt tataaatggt atcagaaatc tatcggagat atcaaatact
     1441 aatttgttat gctgagtaaa tattattgtc ttcagacctt tgttttctga aaggattttc
     1501 cttaattctt ctattttaga tctgctattt agcgcaatgt acatagcttt atttcttgca
     1561 agaattgcct ctcttgcaat actgtttctt ccagacatca ttatcaatct ggtgaaggct
     1621 ccggtgtagt tcattcttaa tcctagcttc ttcagagata cttgatacac accaaagttt
     1681 tttttgtatt cctgaacctc ttctggcaac atctctacct gcctcctttc tatttcataa
     1741 gaagcaaggt gtttgtctct cgcaagatcg ccagcatgtg cctggaacac tataccacca
     1801 ccaaccagtc ttggaaaatc tttatgtaaa ttatcttccc tctcaattgt agccgttaat
     1861 cccaatctaa aaggagaggc aaaccgttcc gctattgacc tgtaaccggg cgcggccaaa
     1921 tggtgtaatt catcaaaaat aatcaaggaa aatttgttac caagacttgc tgctcttaaa
     1981 tatgctgaat cgtatgtgga tactgttatt gcttgaatat catctgatcc tccacccaaa
     2041 tttccaatct tcatataagg aaaatacttt gaaagaacag aggtccattg atccataaga
     2101 tcaattgttg gcactattac tatagaggca gagtttacca tttctatcgc tttcacacca
     2161 ataacagtct tccctgcgcc tgttggtaat actacacttc ctctcttagc tgctttagcc
     2221 cagttatcaa gcgccttctt ttgatagttt cttaaagata tatgctgaga attagtttca
     2281 tctattgaaa tatttggaga cgaaatgaga tctaaaactt tattatcatc gtattctata
     2341 tcgcttcgtt tcagatactc tataatattt tggtaatcca aaccctctgc ccttaatgaa
     2401 tgtgttctcg gatcaactga ggcaaaaggt atgtgaacgt tgcctgttat cagaatggta
     2461 ccattatcat atcttagagc gactttgagc acgataatac gtagtggagt tgctgctatg
     2521 taaagggata tcgactagtc cgccatgtaa acttcattat ttgttatatt ctgacatttt
     2581 cttgacccat caagtcattt acatctgtag ctcaaaaagg agcaatcgaa ttatttcaag
     2641 gcaacttttt cccttattcc tggattctgt ttctgatggt tacttctgnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnngtta cttctgttac gtgcagattg aggaccaagg gatttaattc
     2881 attgagtttc taacttcttt gagccccatg tctaataata gatcatcgta attcttcctt
     2941 gtcatagttc atattaatga aaatgaccta cttaaatgag tagtaatatc taactaagta
     3001 ggtaaacaat acgcagaagg cgtgttaatg actatttctt cgctagcctg aaccatgatt
     3061 gttttaattg gcgatatcaa aaggtagcaa ctatcatctt aaaaatattg ttaattcaaa
     3121 cctag
//
LOCUS       DAOR01000050            3753 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_77858, whole genome shotgun sequence.
ACCESSION   DAOR01000050 DAOR01000000
VERSION     DAOR01000050.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_77858"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1191 a          709 c          689 g         1164 t
ORIGIN      
        1 tcaaacttag catagactaa gaaggctttg catcacaaag acagcaacac ctgccacttt
       61 tttggtgaag gaaagtaatt tgagtaatat aatatcaaat gacaatcaca attgtgcaca
      121 tgaataatca aaaaaccaac cccaggcgga gtatcaattg tataatgagc aagttaacag
      181 gaaaccatag gtttgagacc ctaaaaatgc caattagtga tagagtgggg gttttattta
      241 caatctgtgc caagtgcttg tctaataaag tcgaaattga agtaggtaga ctgaagacta
      301 ccaaatcaat cagcgtacca cccatcgctg caagcacaac ttttgctata aaaggtaatc
      361 caaaatagct aattattatt attgttattc ttattttttt gttacatgag ggcaataatc
      421 tttgacatgg atggtgtgtt agtagaatcc atgccatttc attaccaagc tatgaagata
      481 gcaattaaag aactagccaa tattgatttg gataaaagca gattctattt gcttgagggg
      541 atgcctatta ccgaaatggc actagaaata ttgaagatca aaggatatgt tgatgctaaa
      601 aatataacga agaaggatat tcaaatgtca gaaaatgtag ctaaaagaaa gaaagagcta
      661 tttaagcaaa caaatgtaat accaaaaccc tttgttggtg tcagagaatt aataaccaat
      721 actttgtcag ggtgttcaaa agctgttgta agtggttcag caaaagaaga ggttgataca
      781 atcatagatg aaatatttgg aaaagatgcc tttaacatta taattgatgg tgaccaattt
      841 gaaggaaaag gtaagcctga tccggcatca ttcgaagtag cattgcaaag actaaatgta
      901 agaccttctg atgcagtagc agtagagaat gccccgcttg gtgttaaagc ggctaaaagt
      961 gccggtattc catgtattct tacattaaat acatcacctc ttgcaatatc cgactttaag
     1021 ggtttgatat cggaagaccg cgtatttgag gatacaagct cagctgggaa ctttctaagc
     1081 aattggtgtc attcttcctc tgaagtttga gtacgttcaa aatgttgctc aatctaaaga
     1141 ttcagcttac taggagcgat taatattctt ctcgctttta cctagcaaat ttggaaagct
     1201 gtttggaaat atcaatcatc ttggctacat cctttcctat tggcacaaaa ttataaccaa
     1261 atattatatg atcaactccc atacctttta ctctttgtat atcgcttcct atttcgtcaa
     1321 tagttccgga taacggaaat ctatttttat cgtccttgga tgattgaccc tctctaacgt
     1381 ttggatatgt cagcattatg ttcggaaatt atcgggattc ttattcgatt tacttgcgtt
     1441 gccctttatg gtgtttatac aattttcagt atattccaaa ggacctccga caacgcctag
     1501 ccagccatta atgtcatatt ttactattct agcaaatgtt ttaggactga agcctccaag
     1561 ataaatcgga ggatgtggtt tttgaatagg cttgggctca atttctgaag ccgggatatt
     1621 gtaatatttt cctttaaatt ctatgacatc atcggtccat atcttcttca ttacttggag
     1681 aaactcgtct gctctttctc ctctgttagt aaatggaata ttagaagctt catactcatc
     1741 tttagaccaa cctaggccaa ggccagatat taccctacct tgtgataaaa catctagtgt
     1801 agcatatctt tttgcaagca atatcggtgt ataaaagaac atatctatga cggctgttcc
     1861 taatgaaatc ttgcttgtat tagcagctac gtataccaaa acatcaagtg ggtctaacac
     1921 agtcctatat tcaaccggaa ggctcccgtc aggtgatgct ggatatggtg tttgaggact
     1981 taatggccta agaattcttg tgatagtcca taacgaatca aaatcttctt tctcagcttg
     2041 agttgccaac tgaattacgt tttctcttgt tgcttgtgga ccaaggttgg gtagcgtaat
     2101 gccaactttc atagttgact tggagtagta ctattcatat aaaaagactc cagtatatag
     2161 agagaatatc attcagcggt ggcattttat aagagtggat aaaatgtgtg tgtctcctcc
     2221 tcggcccccg cgaaaaatga gccactgata gtatcctgca gcagaatctt gtagcttgaa
     2281 ttcgagtcta cttttctctt attttaatga caaaatgtct tggtctttct ggagatccta
     2341 tggtttgatc ttcgagagct tcgaacatca tgccagagaa tggagatgta aattgcttac
     2401 ggatttggtc atacttccat gtatgcccat tggtcattct aaaagcacat tgacattcag
     2461 ccagagtatc aaaattcggt ctattgattt cgtttaacaa ccctagccaa ctaccaagtt
     2521 atggtgtaat gcttcagcat aggatcactt tatacaaact tcaaggtggg ttaaaacaca
     2581 tgctgcctat tgcatcgcta caaccctggt gagtatgacc gccattaatt gctacaccgc
     2641 cgagttggat agttgctgaa actctactgc tctatcttgt ggtgtaggaa cattaaccct
     2701 accgcaacag caaactgtgt tatagcgatt gttagatatt aaagttctac cgataagttt
     2761 aatttagaag ggcgaatttt atttcggaac atttcgagag atttatcaat tgtccttcaa
     2821 tttagccaca catttgctag ctcctattgc agttactacg tacgacaagc ataatccctc
     2881 ttacttgtat tgtaaacatt aagagaaaaa ttacgatata gataagataa catttgaaat
     2941 gcagcggaat tcttcattga aatgttcagc aaagtcttta gttgcactaa taaggaaatc
     3001 cttcagatac cggtttagca agtcttgtta aattcatgta gttacctaac ccatttatct
     3061 aatggaataa ggtggacact aatgcattca cagacaaaca tcaccttcag tatagtggcc
     3121 attgcggcac cagtcgtact gtttgccaca ggacccatag ttggaaacta cgcatttgct
     3181 tcgtcagagc agtcctacca tcactgctat caccaccacc atcatcacta ccacaaaaac
     3241 tacaacaact aaacgcgaaa actcaaagac gttggtaata gaaaagacca actaaaatta
     3301 tttttttatt tttaccagag aaaattgatg tatatcatgc actgggagcc ttaagctaca
     3361 gaaattactt acttacttta ttttctcgtc agaagacttc atacaacgga tcaatggcaa
     3421 acggaatcta gtaattggtg tgataaggct tctcctgcaa gtaagatata tcatactttt
     3481 aaagaacatg ggagtacaga aatggcatct ttactttttc ttgattcaaa tgcccgtggc
     3541 cgagtattct taaaaactca caatttagca tccaaaaaat tctgacgcta ggaattttta
     3601 aaacagaatg agtttgtgac attattatat accaaagcag ccagattaat caccttaaag
     3661 ccattgtcat gtagatattc attccttcag caaaagagag atacatagta ctgtattgaa
     3721 ccccactgtt atcgatccag tgatatatta ttt
//
LOCUS       DAOR01000051           31796 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4530, whole genome shotgun sequence.
ACCESSION   DAOR01000051 DAOR01000000
VERSION     DAOR01000051.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 31796)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 31796)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31796
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4530"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4135..4359
                     /estimated_length=225
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11298..11460
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21392..21701
                     /estimated_length=310
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9778 a         5880 c         5875 g         9565 t
ORIGIN      
        1 taccagaaat gcagatgtac gtctgtatgg aactactgaa cattcaagag gattagcaat
       61 tacaagtaac gatgaaataa ttattagccc gtctagacgt actgcaaaaa ttattcgatt
      121 ggattatggc agaacaggtg tagaaggacg ctcttgaata tgaccgcgat cgatatctct
      181 gctgtagaca gctatattca atttattcag aaaaatttct ctagatttgg ggatgcaatt
      241 caagataggt cttatactat attagtaggg ataaaataaa gtatcatcaa tttactttca
      301 gcaagatcgt attctctagc ctaactcgta atatatgtat atgaaaaccg atttaactgc
      361 attttattta attaaaggga ggcttcttcc aggatattaa gtgctgaacc tgcccgaaac
      421 cattcgagct gagattcgtt gtaagaatgt tccaaagata tttcctcccc tcttccgtcg
      481 acgtgacgta agatacagtt gacagacttc ctaggtttga gattatttaa tccaattata
      541 ctaatcaggt catcttccat aattttggaa taatccaatg gatctgcaaa tgtcaatgct
      601 agtactcctt gtttcttaag attggtttca tgtatcctgg caaaacttct ggcaattaca
      661 gccgcacatc caagatacct tggagtcatg gcagcatgct ctctactact gccctctcca
      721 taattattat ctcctattat aatccaattc agacctcttt ctttgtactc tctagatata
      781 tgagaaaacg actcaatttt actgttaaga gtatttttgc ctctccctac ttcaccattc
      841 ctataagcat taactgctcc aagaagtaaa ttatcactta tcttgtctaa atgtcctctg
      901 tacattagcc agggtccggc tggtgaaatg tggtctgttg tgcactttcc ctttactttt
      961 gctaatattg gtaatttctc gaaatctttg ccatcccatt ttgggaaatg ttggagttct
     1021 tgtaacctat cactatcctt cttaattatt acttctacac tgttgggatc cgtagctggt
     1081 gcaatataaa tatccgtgac agctttgaaa cccttttctg gaatttcagg ggctatcttg
     1141 ggaggattta gcttgaattt tgttccatca cttgcagtta attcatcggt aagtggacta
     1201 aacgaaagtc ttcctccaag ggctagagct ataacaagct ctggactacc tataaaattc
     1261 atcgtttccc ttcttccatc gtttcgtcct ggaaagtttc tgttatagga tgtaactatg
     1321 gtattagggt cacctttctt taactcaggt ctgctccact gtcctataca tggaccacac
     1381 gcgtttgcta ggacgtttgc accaatatct ttgagcaact gcatctgtcc gtctctttca
     1441 attgtggctc taatcatttc agaacctggg gtaacttgta acggtacttt actcttgatc
     1501 cctttttctc ttgcttgttc agcaacatct gctgctcgtg acatgtcttc atatgaagaa
     1561 tttgtgcaac ttccaataag agcaaccgaa attgtatcga gatagttatt cttctttatg
     1621 tcttctgcca tctttgaaat aggtcttgct aggtcaggtg tgtgaggacc tacaatatgc
     1681 ggttccagat tagaaagatc aatttcaatc aattgatcaa aatagtttat tgaattctct
     1741 cgattttcac atatttcttt ttctatctgt ggatcttggg ttagcagatt aacgtgttta
     1801 ttcgcaagct cagcaatggc tcctcgtctc gttgctctga gatatgtctc cattcgtttg
     1861 tcatatggaa atactgagca agtagcacca atttctgcac ccatatttgt tatggttgct
     1921 ttcccggtac agctcaccgt tcttgctcct ggtccaaaat attctattat tgcatttgtt
     1981 cccccagaaa ccgtgagctt tgatgccacg aaaagtataa cgtcctttgg agaggcccat
     2041 ccattcagct cccctgtaag atatacacca atgcgttttg gatacaataa ctcccaagac
     2101 attccagcca ttacttctgc tgcatccagg ccacctactc ctatggcaag cattccaaga
     2161 cctcctgcat ttggagtatg ggagtctgtt ccgatcatta atcctcctgg aaaagcatag
     2221 ttttcaagaa ctacttgatg tataatgcct gctcctggtt tccaaaatcc cattccatat
     2281 tttcttgatg ccgattccaa gaattgaaac acttcattgt tttcatagag cgcagctttt
     2341 gtatcaggat cacttcctat tcttgcctga ataagatgat cacaatgaac tgttgttgga
     2401 acagcggttc gttttaggcc agctagcata aattggagaa tcgtcatttg ccctgtaaca
     2461 tcttgtaacg caaccctatc tgggttcaga agaacataac tcttgccagt aattaacgcc
     2521 tcatctgaac caaagtctgc actctcgtcg agatgtccaa tgagaatctt ttcacataat
     2581 gtgagaggtc tatccctagc ttttctaaat gttgagatct tgatttgtaa tttttcatat
     2641 actttggtaa ttaagttggg tgttgtttct acctctattt tcgaattaag attggacata
     2701 ttagatctct tattatagcg caattaggtt tatatggaca tacttatttg cattgttctc
     2761 ctcctccttt aacattcccc cctttggaag tcttgtgttt gttcctttgg tatcaaagca
     2821 tcttcaacct aggacaagca gaaacacgat ctgtatcccc tgtccataaa gggagccgac
     2881 agtgatgcgc tacgtgagag aggaccaccc tgttgcgcat agatgccagg gtttggatcc
     2941 aggcttttta gctaagacag tgttattctt ggagttggtg cgcataccac ctggttcaat
     3001 caatactatt ttaattccaa agggctcaag tcaatgtgtc gacctccaag tgcagggaga
     3061 attaatagta ctaccgtaat ataatgaaga aactagaaat ctcacgtgga tatgacccat
     3121 tttatgctcg agcgacttaa atatacttta tatctgcgca ggaagccaat tgtttcagtg
     3181 gttgaaccat gtctttggct taatctcaga ttattcacct tacataataa tagcctagag
     3241 aacgcagaac gcaaaaaaag cagcaggaac gtggatttgc tttaatcgat gacaaactgc
     3301 aatgcagaac ataatcatta aaaacgagca atatccttga gtacctattt actgttattc
     3361 taatatactc tttaaaccta caatcaattt atcaatatct tcttcattat tatagaaatg
     3421 ggtagatact ctaataccct tccctcttgc tgatacaatt attttgtttt tcttctctaa
     3481 ctcttgaaca acgccaacag gctttttctt tgttatttta aacaaaacta tccctgatct
     3541 atattttctc tcttctattg gcgaaagaat ctgcagtttc atgttttgaa gattatcaat
     3601 taaatgatct gttatactga gaattcgttt ttcaatatgt tttatcccaa tattggatat
     3661 gtatcgaagt gcagcattta atgcaactat gtttggaaaa tggggagtac ctagttcaaa
     3721 tcttgttgca gtattggaca tattaacatt catattgtta tcaaaatttt ctcttctttt
     3781 ttgaccgaat tgactaaaaa acggtggctt aaggttttcc acgtctctta aatacttcct
     3841 tttcatgtaa atagcaccaa ttccaaaaga cgatagcatc catttatgcc cattcgcggc
     3901 cataaaatca attccaaaat ccttaacatt aaaatataat gctccaagtg attgtgtggg
     3961 attcactaca aaataaagac ctttttcttt tgttaacttg ccgagctctt ttagatcttg
     4021 tctgaagcca gtagaatact gcacatgact ggtgaccaat gtttttgttt tactattgtg
     4081 gtcgaccatc ttcgcaatat cttctattat tattttatta tcatcccttg ccttnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gatattacaa tgcctttatc
     4381 tgacagcata tgagcaatta tattcatacc ttcgctagtt gaatgagtaa atgctatctc
     4441 ttctctacta tcagctccga taaatttcgc atacagatcc cttgtctgtt ccattttggt
     4501 ctcccattca tcccataatg tttgtccgtg gtttagtacg tcttggtaaa atttcgaaac
     4561 ttcgttgtat actggcatag gtataggagc aatggctgca ttagcaagat atgcgtattt
     4621 ctttgtaact ggaaagtcgt ctctaagttt aacccaatcc aacatctatg ctacagctgt
     4681 agcatcattg agtttagatt ataaatattc tgtagttctt ttgaaggcgt aactgctcaa
     4741 aattgcggca atgtcattat catagcagcc tagcgatagc atttataagt caatcaccga
     4801 ttattcaagc gcatgaaaaa tgataaatta agatggggcg gcaataacaa tagtgatttg
     4861 gagttttgta catgtatatt caaataaaag tagtcagtta gctaaaattc tattctttta
     4921 taaaatatct tctttactat ttctgccgta acaacataaa tcaaaagaat tatccctatc
     4981 gcatagaaat acaacataga gagacttgta aaaccaaata gccctgcaag tggtgtggaa
     5041 agcaaaatgg cgacaagacc aatgataagt atagtggcca ataaaaggtg cttccccggc
     5101 ttacttttaa acagaggttt cctacttctg ataacaagta caataatgga agcagcaatt
     5161 ggtccagact caatatacca tgctgtccta aattgacccg tagaagaatg taatatgaac
     5221 aaaagtacgc caaaagtaat ataatcaaat atcgtactta ataaaccaaa tactatcata
     5281 aatttcctaa taaattttat attccatcgc ctgggcttct ctactaattc tgcatctaca
     5341 ttatctgtgg ctattgccat ctctggcata tctgtcatca aattcataag caaaatctgc
     5401 tttggtagca atggaagaaa tggtaagaac agtgacgccc cagccatact aaacatagtt
     5461 ccgaaattcg aactagtggc cataaagaca tacttcattg tattggcaaa tgtcctccta
     5521 ccttcttcta caccttttgc taatactcct aaatcctttt caagtaacac aaaatcagca
     5581 gcttctttaa caacatctgc agctgtatcg acggaaatac ttgcatctgc agcatgaagt
     5641 gctgaggcat cattaattcc atctcccaga taaccaacaa cattttttct tgagtgtctt
     5701 aaagctaaaa tgatttgttc cttttgattt ggttctatct ctgcaaaaat atcgatctca
     5761 tcagcttgtc tcattagtgc ctcgttgctc atgtgatgta gatcaggtcc tgtgagaaca
     5821 cgaggattta atagtcctat ctcctgtcct acgtatgccg caacatgtct gttatcacca
     5881 ctaattattt taagagagac accaagtctt tttaaattag atatcgattc tatgacatca
     5941 ggtttagctg gatcaaaaaa gatgagaaag cctaggaaag tcatatcgat ttcgtctgct
     6001 ttggtgatca aagaacgcag agtggtaatc ttattattat tagtgtcgtc atcatactta
     6061 atcttacgat agcaaacacc taaaatacgg aaacctttgt cacccagttc ttcaaattta
     6121 tcttggattt tttgtttgac tgtagataat tcaacaatat ttccatcagc caattcagca
     6181 tatgaacata cctcgaggac gttatgtaga gcgcctttag tgaccataaa aggttgtgtt
     6241 cccgtcgttg tttttcttgt taataatgtc ttggatttat ctgagaatga tgaagatgag
     6301 gtgatcaaaa tgcttaatcg tttacgaata aaatcatatg gaatttcatc caatttagta
     6361 tatcccgata tatcaaattg ctgagaacaa aagtccttga tggctgtatc aatgggattt
     6421 gagaaaccag tttcatagac ggcattaaga taagcatgaa gaagaacttt attgttagta
     6481 ttgccttcca catcaactgc agattgaagc tttacttcac caattgttaa tgttcctgtt
     6541 ttgtctgagc aaagaacgtt catgcttcca agattttcta tagatgcaag gcgttttaca
     6601 attacttttt cattggccat cctttttgca ccgtgggcta ggttaacact aataattgca
     6661 ggtaaaagtt gcggtgtcag gccaatagct aatgctaatg agaacaaaaa tgattctatt
     6721 atgggtcgac caagatagac gttgattaca agaatagaaa tgactaacat cagtgttact
     6781 tccataagaa aatatccaaa acttcttaca cccctttcaa attctgtctc cggtgcctta
     6841 cgccttaatc tgtcagatat tttgccgaac tcagtattag ttccagtttt aatgaccaat
     6901 acttttgctg taccactcac tacaaaggtt cccataaaaa tagagtttgt tctttctcta
     6961 agtggcgttt ctcttggcaa tatatttgca gatttctcta caggatagct ttcaccagtt
     7021 aacgtggctt catttacaaa aagatctttt gattcaatga ttatacaatc tgcgggtata
     7081 ctgtctcctg attttagtat aactatatcc gcaggaacaa catcttcaga aggaatttct
     7141 tgtggcttgc tatcgcgcaa tactgtagtt tttaatcgca caatttccaa taatttagta
     7201 atggcatctg tagctccctt ctcctgccag aatccaagca gtccgcttac aattacgatt
     7261 gaaataatga ttaaagcatc ctctgcttga ccaaggaaaa atgacaaaat agaggtgaag
     7321 ataaaaataa ctattattgg acttttgaat tgagagatta gtaacgataa tgaatctgtt
     7381 ttcttttttg attctaccat gtttttacca tacttcaata gtctcagatt tgcttctgta
     7441 gaagttaatc ctacttcttg ctgttgtcgt cttcttgctt tttcagttat agtattatca
     7501 aactgtcgaa aaagatcagc agtaggaata ctccaataat agtctggttc ttgattcgtc
     7561 atggcggtag ttttatcaat tcattaataa tgatctatac gtatttaaaa gccgccgccc
     7621 ataccccgtc cgttaatcgc ttcttggttt acctcttcct tcgcttcacc caggatcaac
     7681 catagccgtt atatttaaaa gcacaaagta aattgtatat tctcatgact ctaaacgtca
     7741 gtgatatgat gacgaaaaaa ctagaagcaa ttgaaggaac cgcttctgtt caagaagccg
     7801 ctaagaaaat gaaagatagc tacgtgagtt ccttggtggt agttgatggg gagggtaagc
     7861 cacaaggatt agtcactgaa cgtgatttag tcagaaaggt atgcattaat gatgtaccta
     7921 ctagcagagt gacaaacaaa gagataatgg ttctggttcc attcccgatg gatggggcat
     7981 agatgtttgg tttttaattg aagcagctat ctcagggtac cacattaaag agatatttat
     8041 gggcacgaag gaacacacat tttttgaaga ttatcgagaa gatgtttcca aattaagtaa
     8101 gatggctgag caagtagaat ttactataat aagggaagca atcaagtatg atagattaga
     8161 attgcaaaaa agggttaatg tttagtttta tacgctgaac tatatctatc tattaatatt
     8221 atatacttct aacgcttatc tattactttg acaggattgt tcagattaag gaatgaagca
     8281 cctgaaaatt aaatttctaa cttttccatg ataaattaaa agccaaccac gcctagaaat
     8341 cccacgacgc tggacaagtg ttctgtatag tttaatcatt attgctcctt agtaattaaa
     8401 tcgattcaga aattaatttt gccagttctt cttttcctag tctctggtac agtttcattt
     8461 gagaaagcag ctgctctttc atctgcttgt aaagattgat ccttataagc actgactgcc
     8521 tttctatatc ggctacttgt cgttgatgtg atgacatact atgctattct ttaatgtgta
     8581 tattataagt ctctagtctg gattaatttt ataccgcaat taatatcata tcctacgcat
     8641 cgtggactaa ttactcctgc acttttgacc ctggtagagt agagacattg attgcgtgat
     8701 ctttagtacc ttctgcagcc aaatttgaga tactcgttga tataaatcct caaaggaagc
     8761 tcatctctat ttcctagtct ttttaccata aattcaaatt tactaacttt ttagatggga
     8821 agcttacttc tcttcgtgac gaactgagta gtagtattgg tggcattcat tttctaacta
     8881 gcataaaagc ctcttggtat accaaactaa cgaaaaaaca cacacctaca caacaatcac
     8941 aaacccttct tgtatctgta cttgttcatt atctctacta cctgctcatc actaacttga
     9001 gaaaagtctt cataaaatgc tccaacagcg gaaaatacta atgctgactg taaaacaacc
     9061 acatcgtcta ctttttgatc ttctttaagc ttatcaaatg tatcttttgg tgccacggga
     9121 actgcaacta tcagtctttt tggtttttgg tttcctaacc attgtataac agcaaacatt
     9181 gttgcaccag tagctatacc atcatcaact aatattatgg ttttatcatg taagtgatat
     9241 tgtttacttc ctctaaatct cattaaacga cgttctatct cctttttttg gacagatatc
     9301 tgttcatgaa catactcttg tgatacattt agcatgttaa ttacatcttc attcggaaag
     9361 aatctaccat cagacattac cgcacctatc gcaagctcag aattgtaagg tgctcctatc
     9421 ttccgtgata caacaatatc taatttagcc ccaagactgg atgccactac atctccaata
     9481 accactccac ctctaggaat agctaagatg atgagttcat tagcagattt ctctaccaat
     9541 aacttcaatc tatcaacaag tcttttggcc gcatcttctc tatgtttaaa tataatcact
     9601 cgagctattt tacattcatt ttcgaataat aatctatagg tgttagacaa tgttaggagc
     9661 aattaaaccg cgtcgttttt gtttaacata caagtagatt attcccaagg atttctaaat
     9721 gatgcactta gagatggcat atgaagagag tgtgttgtta tttcaagggc tagttaccat
     9781 ctgcctgaaa ttgtccgagt ctggtctccg ctttcatgag tagcaagggc tttataatag
     9841 aaacaggaga tcaaaatacc tattcgggct gtaaagagaa gaccaacgtg taattttgca
     9901 tctgtgcttt gaacttgacc actaagccaa attcaaaaga atcacaacat gacgaattag
     9961 atggaagata ttttaagttt aacgttcaaa taacataata gtctactaga tgtccaaatg
    10021 gcaaaggacg tctttgattc tagtactgca atagagatac gagatgcttt gaatggagta
    10081 ttgctaaact catcaagatt taattggaaa ctttatgaag cattcgtcga ggcttattca
    10141 gtttacttga atatactctt caaagaagat aatacttcaa aagacttggc agagctcgag
    10201 aaaatcataa gaaagaaaat tggcaatata tttaacacaa aattcagaga tgataatttc
    10261 atcaccatgc tttcaaatgt cgttgctagc tattcaaatc tggctaaggt cacaggcttt
    10321 gggaagatat atcaacacct atgtaatcta tggtcggtat gggataacga ttttgtagaa
    10381 ccattaagag atacactatg gagaacacct tcatataaga ttactaactt ggagaaatac
    10441 tctttattcc gttattatta tgatagaact agagaaggag ataagaacaa cgatagaata
    10501 cacccaacaa cacctctgtt aatagtctat gctttcataa ataggcacta catacttgat
    10561 cttcttccag aaataagtgt agtaggaaat ttgctaaggc aagggtttga tgtttttgct
    10621 acagattggg gtaccccttc tgcttatgac aaggatttga ctataggtca ctttgtaaat
    10681 agctatatgg atagatctgt tgatctgata agagagatta ctaaatcaga taaggtatct
    10741 ttgtttggct attgttgggg tggcaaccta gttcttattt atgctgcact acatccagag
    10801 aaggttaaga atgtaataac aattgccacc cctggagatt ctagccttga tgatacattg
    10861 ttaagcgttt ggaccaaggg catggaggtg gatacccttt tgaatgcatt tggaaacgta
    10921 cctagtacac tattaaatga agcctttgct ctcagaaatc cagtagaata tagctataag
    10981 tatcctcact tttttgaaca atcgcatggc ctagaatcca taatggaatt tcttgccaca
    11041 gaaacgtggc tatatgatag ccctcctata ataggtgaaa tatatagaga atttgtaaag
    11101 tattgttatc aacagaacct gtttatcaga aataaaatga tggtagatgg ggccgttgtc
    11161 aacctgaagg atattaatat gccatttcta aatgtagtag cgcaaagaga tgatttagtg
    11221 gccgcacctt cgagtattgc cctaaacaat gcagttggaa gtgttgataa gagtgctatt
    11281 gaatttcagt caggacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 gtatttattt tctttgctat agcagcgcag gtccctgctt cgacaatttt tgctctcgat
    11521 actaaaaaat gctcatttgg tttcaaccta ttggcacaac atgctcagat tggagtagtc
    11581 atggttggtg gcataataat tattctattc tctatatcgt cagtgttagc tcttatacat
    11641 cgtagtatag aggcagctat agcaacatca acggggtttg gggttatgtt tgttgctgca
    11701 ataataattg tgtcatcatt agcatgcagg cttcccactg atttttttat tgatatgatg
    11761 gtggtccctt ttattgttat tattattgta attgtaggtg tgcatatgta ttctgaaaga
    11821 aaaagaatta ggaatatgac atgagatcaa acaaccaaaa ctacaatgat atctaatata
    11881 tgtacagtca aagaaagact ttccaaactt tagggattat aatttttgtc agatagaatt
    11941 ataaacatta tatcaaattg agtgtgttgc tcagccgcca acaatatgat ctgactttag
    12001 tgttttcatc acaatgtccc acggggtgac tatataatta tgctcttctt ctttttcatt
    12061 gccttccaag atcaagcatg gattgtgaac atccatcaaa agttctgccg cgctacttac
    12121 agtagtattg gatttaacag tagttgggga aatgatatcc aagttattat tgatattgat
    12181 gtctgctaag cctgcaatcc cattcttgcg caaaacttcc cttccattat gactcaatag
    12241 aaactcgagt atctttctat catttataac atgaggcaat ctggattttc tactactttc
    12301 ggtaccacta acaccgccac tatcatcatg agcaaagagt tcttccttta ttccgatatt
    12361 tctaatacct ttattcagca tatagtctaa tgcatccctg acgctagtct tactatctac
    12421 ggatattaat tgagaagaga gttcctttat tggtatgcta agattagctt ttgcaattag
    12481 cggtagaata tctcgtattg caagtgatgc tgttactact aatgaatctc cttcttcatc
    12541 ctccatccta tcatcctttt ttgctaatat tggaataaaa ccaaaccttg tattctcaaa
    12601 gacttccaat gctcttttca agggagaatc catttctaaa ggcattacaa agccatccat
    12661 tatttgtgat gcacttgtcc ggaaccagtc cggatagcca gagtctaaaa tgctggaaat
    12721 aatatgttta ctactaattc tacctatggg tctccctctt tgtactttat cgtcgtcgat
    12781 tactattact ccatccacat agatttgagg ccctattgca agaaatgttg ctatttgaag
    12841 caatcttgta tttggttcaa caaacaaaaa tggccgtctt aagatgtgag gagctgcatc
    12901 ttttatttga atatctaata agtggttcat acaaatctcc aaatcattat tgttactacc
    12961 gccccatttt aatcattaat tgttttttta ctaatactac cgcccttcgt ttgcttatgg
    13021 tctccgcaaa atacccgaaa accaagactg accaaattga tcatagagtg ttttaggttc
    13081 tattagcagt ttatcaccta cccagttcct cacggcatcg aaatccatga aggacttcct
    13141 cttacgatta gaggcgagaa gcttcgcagg caggtatggt ttcgcattcg acacaagata
    13201 tgacagtttc atggccggaa gcgctgcaaa gtatatcgag aagaggctag agacgacagg
    13261 catgcagata atcaatcctc atacatccct gatagtcagg agagggtttt cagaagtgaa
    13321 gtgttctggg gacatggctc ggtaaccagc tcatgacaat tcaaatgtct tgagcttcgt
    13381 tctggccatt atctagcgac tagagagcat agcgcattac aaatgaagtc attgtagaaa
    13441 ataaatatta gctaatgtgt taacgaaaca aaacatttct tagttcttgt aactgccaga
    13501 caaattgttt catgtggttg ttgtcggttt tgttcttgcg cctcttcgtc tatccaatat
    13561 agctactata agccctgtta tcagaacaat gattccaaaa ataattagga ttcctcctac
    13621 tactatgcca ctaaataaat ttacattaaa ttggttgttt tgacctacga aaggaagatt
    13681 accaaacaat ataccaacgc taacagggtt atttcgtaga ttattgatag tgaggatgta
    13741 ttttcctaca acgtctgttt tcaatctagt caagaattga tttgagaatt cattagccga
    13801 tattatctgt ccattggggt ctcggactgt ttctcttagg gaaggatttc catttgattc
    13861 taaatgtatt actaaagata aaggatgatt tatatcactt atttggcgta agaagttcat
    13921 aataattata aaaattgggc gagaatgaag agcaaattga atcattcatt tttagttgca
    13981 tgaatggctc gaattcagat aaaatcgtga gcttaccaat gaattatttg atataggaca
    14041 agataatgat ttaatcgacg ctatgaggtt aaagttgttt taatgtcaat tacagtggga
    14101 cttgactatt aacagcagca agtgcgtttc ctgcaaagag atttatatcg agacgattca
    14161 attctatgac acatatggtt gatttagatg ctcttcagtc tatcatttga acagctttac
    14221 aactttaata ccagtgttta ttgaaataag aaatactccc caagctgtta attctgctaa
    14281 tgtgagagcc tgatatggtt gaacagatga ttgtagcaag gaataatgaa tatataactt
    14341 tgttactatg aagatgattt cagaaactga aaaagttcca aataatttct ttgtgtcttc
    14401 ttttatgttc ttggaatttt tctttcctgt taatggatct acatatcttg acttgttatc
    14461 tctataaaaa agaaaagcaa aaagtggaat ataaacacag tatcctatta gtaatgtaat
    14521 cagtgcatta gagagattat tactatcaaa tagtgcataa atctgcgtga taagagctcc
    14581 agcaagaaat gaaataactc cagataggat taagttctta ttgaagagta ttaccttcct
    14641 gtacctttgg aaaaaattct gttttcttat ggccatttat ttatcgataa aaatatctat
    14701 aagcacatag ttaaaattct ttatttgtac ttttcattct tttgcatggg aagatattat
    14761 tgtcttataa tattctgtca tacaaagtat tatttaagtc atgatgtata tacatccaca
    14821 tattatggct agacgttcaa agaaatcgat agccaaaata ttaacacctc gattactgcc
    14881 gccgctagta gcacaaccac catcatctag aaattatgat aattatgatt tactagaacg
    14941 aaaaatcact atagcaactg aaggctttac tacagtcaag ttttgtgaac tgatactaag
    15001 ggatagaaat aggctatcta aagagaatgc attaacgata tgcgaataca tgatagcaat
    15061 gaaaagagaa atcaacccaa cactcagtta taaaagaaat accatacagt ttttagcaga
    15121 gttgtcaaag accgttggaa ttgcaaagaa gttcattgat atggcaagag acagtatttt
    15181 ttgctattta gacaaatgtc gcaagctaga aaacgaagac ccattacaca agtggatagg
    15241 tagttacaat acaaaacttg tagtattatc tcggtttttc aagtggctgc attatcctga
    15301 cattgacgac ccaaaaagaa gaagtgagtt atcagcactt gagagaagac ctgattgtat
    15361 catgggtatt agacaactca aaagaaaaga gatcagctgc tacaaacctt ctgacttgtg
    15421 gtctcaggaa gacgatttat tgtttctaaa atgggttaca aacaaacgtg accgctgcta
    15481 tcatacaatg gcaagagact tatcggctcg tccccatgag atattgaatt tgaaaataaa
    15541 ggctatcctc tttaaagcaa ctgctgataa caaacaatac gcagaagttc tagttaatgg
    15601 caaaacaggt acaagacata tccgctaatc caatcgatac cttatataaa agattggcta
    15661 tcaaatcatc cttgcagaaa caatccaaac tctcctcttt ttgtagcgtt aagtaagaac
    15721 tcgatgggaa agcaacttag cgttaaaggt ctatatggaa tttacaaata ctacaaagaa
    15781 gagttctttc caaagctact tgaagaacca acagtatcca gtgacgataa agaaaaaata
    15841 aagagtcttt tagtcaagcc gttcaatcca catacggcgc catagtgctt taactgaaaa
    15901 aagtatcaag ataaaatcca gcacgctaaa ccaacacgct ggctggagca tgaacagcaa
    15961 tatggcccaa aaatatatac actatttcgg aaatgagtct tctgaatcac ttctagaggc
    16021 atatggcatg gtgactaaaa ataatgtttc tatagatacc ttaaatccta agttatgccc
    16081 taactgcaat gaaggtaata ctcaagatgc taaattctgt gctacatgta gaatggtcct
    16141 gacctacgat gcctatactg agactctgga gaaccaaaaa cagaaagaag acaaactagc
    16201 aaccatggaa gaacaattta attcaatgca atcacaaatg cagacgttaa tatcatcact
    16261 ggctactatg gatcaatcaa ataaaaatgc gtttgcgaga caactatttg ataaaggtgt
    16321 atataaaaaa gatgagctta cggataagtt atagatatga tttggaacca aagatgtttt
    16381 tgcaaatcct atataatttc aatcagtaat atacaaatat tgatataaaa cccacgttat
    16441 tacctacgtt ttacataaag ttaaaaggct agctattcgt agcatactac agacagacac
    16501 atgtcttcaa aaaataaaaa aaaggatcag acaccccttc gatgtaagag gtgttcgcat
    16561 acttggcaat atagcggaac ctatccttac gtagcaacct gcccacattg cagaaccacg
    16621 gtttctattc aaaaacacag agtcagtcag accggtcagt ctttggaacg gcccggccag
    16681 tctggtgacc tcacaaccat cacggatagt atccccaacc atgaatagtt gcagccctat
    16741 atttggatca agtgtattta aagtttcaaa attctctacg gatactgtta cggaatgtct
    16801 tacgagcgat tgcaaagatt gcagcggttc gtacagtagt gaaatcctaa ggcatcgttt
    16861 tgtttgcaca tgtaactgtc acaaccaggc ctataacaat gaccgtgaca ggaagaagaa
    16921 aactcacgca catcgaagca acctaagaat gcaccgtgtc aaagatcaag aaaacaaagg
    16981 tgaatatcct aatcatggtt gaagatgaaa gtaataacat tctgcagcgt atagcgcttt
    17041 caagttcaaa ttcaaacaga acgcaactag ggacagaacg gctggactgt gctaagaagc
    17101 cggaaactaa atactcaaac aaacggtgga gtccactatc tgcgtcttat gacaattacg
    17161 tgacatctgc agcagcaccc gagaaagcag caatcacacc ctggactact gcgaaaatga
    17221 tagacgagct gattttggaa cttgctcggg taaatatcta cgtcaaaaat caagaagacg
    17281 gattcgttcg tatcatccct aagtattatt cgcaagcgca agaactcatg tataggatcc
    17341 cactctacaa accggaccca ccttactttg agatggacct gaaggatctc tcctgccaat
    17401 taaaaaatca attagccaat atcaagaaat cggatcgcac gatgtttgag tatgttagaa
    17461 agatggcaat cacgcacatt tttgagaaca ttcagtttgc gtatactcac atatccgtat
    17521 gcatcatctc aggatccgat ttgtagacaa gtagtgaaga aatgaatcca gaagaagaca
    17581 aaaagaccct tctagaggac agcgaggacg caagtacgtt accatcatcg gttaacgtat
    17641 gtaccgtgtc tgccgccata agaatgcatt ctggcgatgt cgaggttaga ggagggatgc
    17701 tttgtggcat aactgacaag gtcgagaaaa tgatgataaa agtatttttt aagtgtggac
    17761 aatgtggtac tattaacgaa ttgtactcgt acacacgccc taggttcgca tacgaagtcc
    17821 ctgttaaatc ttcaaagccg ctaaaatgcg tttgttgcga tcaaggtact ttccgcgagt
    17881 atgactataa agctgtaaat gcgattaaga tagaattgca ggatgtagac acttttagcg
    17941 atttggaaag gttgtcggtt atcttatttg atgaccagac aaataacgta tctgcaggtg
    18001 agcatgttat catcacaggc tcgattcaca acataagaaa gaacggcaag ctcttgccat
    18061 tcttatttgc tgattcgata cgctatcaga atagaaagga attggtcatg acgaatgatg
    18121 acgtgcagga gatcaaaaca tttgcaaaag aggaaggtaa cataattgac gctcttgtat
    18181 ccaaatttgc tccctcggta attggatatg aaagtgtgaa aaaaggcttg ctcctttgtt
    18241 cggcaaacac cggtgaggac tctgtttcca ggagacttcg gattaatact ttattggttg
    18301 gagagacagg attggacaag tctgcgctgc tgagggcagc tgtcgagctt gtcccaaata
    18361 gcaagtatat cagtactctg aattcttcgg tcaggagcct gatcgctgtt gtttctaaag
    18421 atgatgagca ctatatgctc agaccggggc cggttccagc agcaagcggt gcaatttgtg
    18481 ctataaatga aataggccgt atgaactacg aagatcaagc gggatttttg gacgcaatgc
    18541 aggaaggtaa aataccgttt gggaagtacg gattcaatac aacgcttaaa gggtctgcta
    18601 ccttcgtgat gtcagcaaac ccaataaaca actcctcctg gagagatgca gaaaagatca
    18661 atctcaacga gattccggtt ataatgccac tgctggatcg gttcgatctc tttttcgtat
    18721 ttaggaccgt acgcgaaaag gcgaagatag cagaatatgc attcaagaaa accgatccta
    18781 gtatgatgtc ggatctcttg cacgaacagg aggataaaaa ctacgacttt ttgaagaaat
    18841 atgtcatgta ttgtaagaga ttccacccca aattatcaga agaggcaaga atcatgctgt
    18901 gccaatatta tgtcatcatt gcaaccaaat tcgggtcacc aagggtactg gacgcattac
    18961 ttagaataag tcatgccata gccagattaa accagaaaga aattatcgac aatgaagatg
    19021 caaaagaggc gatgcaattc tacaatgtta tattgcagca actatcagaa gtggtagcca
    19081 ttccaaaaga tcctcgtgat ttagcagtag aggaaatcac caatacctta agtaactcaa
    19141 aatttccgta tgattttgtg gaactggtga aagacgtttg caaaagagat gaatcaatag
    19201 cacagtatat aggtgataat ttctctgttg tgaaaaacaa gagactcaga aggcttcgag
    19261 atagattcaa agaagggaca gacaaccgga tgataatcgt tagtctgaaa ccgttggtac
    19321 ttgcatggcg ttcctcctac aatggtagcc gaagtggaag cgctgagaat ggtagcagcg
    19381 gtgaagaggc agatagttcg caagaagaac aatttaagag tgaaattcta cattcaactg
    19441 acctaactga cccaactgac caagagaaaa cagagatgat accaaaaaat aatattcgaa
    19501 atcacagtgc agatggttct ggcaggttag ataggtcagt tggttgcaac acttctattg
    19561 aggattcttc tagcataaaa actgttaatt caagaagggg ctgctttgga tgtttttatt
    19621 gtaccgaatg tcattcaagc gatcaagaac gtactattca tattgacaat gcacatccaa
    19681 ggaagctgca ttatccaaca ccgaaatact ttgagaatag gttaagacca aacaaataac
    19741 aacaagatga ttcaatcctt cttactttcg tcaactactt cattacaatc atttcgtaac
    19801 aataggtgta agccagaagt ggtagcgatc gaataaaaat gaacaaagat aatgaatacg
    19861 aaattccaga gaatgcaaca atcagacagg aacacgttag atgtggtaat cctgattgcc
    19921 aacaattgca tgggccatac ctttatgcgt actggaagga gggcaagaga ttacgaaaaa
    19981 aatacatcgg taagacactt gccttcttga gaatggatgg ttggttttca tggccttcct
    20041 cccgggaaaa tattgatccc gtacaacaat tacattatcg tatgtggcaa aaggggggaa
    20101 aggtagttgc tagtactata tatggcatat cgctgtcagc tgtcagcatt atttggaata
    20161 gtctttgtat atagtaggaa ttcgttgcct ggttctaaca acaaaaagaa agcgcttgtt
    20221 ctgacgggta ttatgttcgt tgcattattc ttgattccta ctcttaaata tccagccaat
    20281 ctacctgctg tgggaaatcc agatacaata tactacagag agagcttgta tgttggattt
    20341 atagccatct ctggatttag tatcttggca cttgctcttc tatacacaaa aaaacttgta
    20401 aagaacacta acaggccttc tagatcaaaa gaaaacatcg caattccagt attgttgtat
    20461 ttagttataa tgggtagcac atatcttttg tttccaccta atcctgataa aatcacaata
    20521 ttaatggatc tgcttatgac gtttagagtt gcaggtgctt tcactattgg tattttctgg
    20581 ggtttaatgg ggttaattct aggctcattc tgggatatac tcaaaccaca tgaatctcgc
    20641 aaaattgcat cggtatagta caagaaaagg ctcttcaacc acgaccaata ggacaaaaaa
    20701 attaaacatc cgtatggtgg agtatcttta cttttataca ttcaacgacc agattatacc
    20761 taagttttaa actaattatc tatgccgata cgcaatcacg acggttgccg ctacagctgc
    20821 aggaattcca aatatgacag catactctat gtaaataatc gttgaataac ttgctgtgaa
    20881 aggattgttg gattgtctat tggcagcagc tgctagctga tttgcagcag ctgaggaaat
    20941 attaggatta tcaaatacta tagtattaaa ctggatatct gatgatttcg cggcaccaac
    21001 attttcaatt ctgatggttg taggatcaga atttgaaaac acataatgtt gaacgttcat
    21061 tccagcttga gcaatgcttg gaatacgttg aagttgtttt tcaccctgta ttagtacaaa
    21121 atcatatggc aaaagatttg gtttattgtt tgcaagatca aagaaagtaa caaagaaaga
    21181 tatctcttta ccagtaagca aaacgttagg atcccaactt aaccctgcct ggatttttcc
    21241 atcaggggta aaattgtaaa ctggcaattt gcttattctt gcaccagaag cataaacagg
    21301 ataattgggt atgttttgtg cctttcctaa ttctgtgaac tttatggata tcaacatttc
    21361 atgattattg tttcctgttt cttgtttctt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtttcttg tttcttgttt
    21721 cttgtttctt gtttcttgtt tcttgtttct tgtttcttgt ttcttgttga cccagctagc
    21781 tacactctct aattgagttc tgttgaatac tgcttcagct gcctgttccg ctcaccgaag
    21841 gtcactatta tgataaaggg gaacgagcaa acacggactg gctcgtaata gcctctttgc
    21901 tagcaggttg ttttctagtc ctgttaaaat agtagtttac taatgtcatt ttgtgcatcc
    21961 tgcattattg gctatggcaa tacctgccct atcacctgag attactctgg aagaatttca
    22021 agctaaatct ggattcatcg gtaagacaac ggcaagaggt gttttagaat atttggtcaa
    22081 gagtgggata ggccaaattt ctcagtatag ttattcattt tctaaagtgg acagaatgaa
    22141 acttgcaata cttgcactcc aaaggggaaa cgacatagaa acaatttcta gatcattaag
    22201 ctggcaggat tttgaagcgt tcgcttcaaa tcttctcaat ttgtccggat atgtggctga
    22261 atgcaatctg cacttttcta agccaagtag aatgcaaatt gatgtagttg gagtcaatta
    22321 taattctcaa ttagctatcg tagctgattg taaacactgg aagcgaaacg acctttcatc
    22381 tatatcagct tatgcaagaa ggcaagctga gaggacatcg aaattattag ttcatagaag
    22441 aaggatctct catgccattc ctattatatt gacccttcat gcgatggata taagatttgt
    22501 tgagggtatc cctttggtac ctgtagccaa attcaactcc ttcatcgaag aagttccact
    22561 ttatctttct gagatcaaag tcatatctgg gattcctgct catattgatg tatgatctac
    22621 aattaagcgt gcgttaaaac tcatatccac gtccggcaag agagaatttc ccattatctg
    22681 taatttttag attttgtgta ttggtaacta ctttgttaat aaatgtataa tgggaataag
    22741 gaacaaagta gcattggttg gtattggaga cgcaaatgtg acctgcaaag tataatccca
    22801 aagcctatcg gtgggatgta catgaagcat gaaacaaatg taaattgtac atttgagaga
    22861 atcttctaaa gttggtggtg gcgctccttt cttttctttc aacgctacat taatcaaatc
    22921 cccattatgt aaagactcca aagtctgcaa ttctttatac taggaaagat agaggtatga
    22981 tacttttaga ttgtttaaca acgaatttaa caatcacaac cagtttgcac ttaacggaag
    23041 ttatgaaaag taggacgaat tacaccccat ttctcaggca atcacataaa taaaatgtaa
    23101 tgaccgaggt aggctaaagt ggaatgatca atagtcctat caaacctcct ttcaatgaag
    23161 aaacagctcg tgcaaaggtt aaagctgctc aagatgcatg gaatacgcgc aatccagagc
    23221 acgtagccca agcttatact cctgattctc ggtggcgtaa tcggacagaa ttcttcacag
    23281 gtagacaagc aataatagaa ttcctaaaac gtaaatgggc taaagaattg gattatcgtc
    23341 tgatgaagga actttggtgc tatactgaaa atcgtatttc tgtaagattt gagtacgagt
    23401 ggagagatgc ggataatcaa aagcagtgga tgcgtacaca tggcaatgag cattgggaat
    23461 ttgatgataa tggcctcatg cgcatacgcg atatgagtgc taatgactat cctatcaagg
    23521 actcagagag gcgctatcgt tggtagcaac caagctcctg atcgaaatgg aaaacgattt
    23581 tctgatgcac ttgaagaccc ataactacta ccactagcta ccaccacaga atgtctaatc
    23641 atgattaaac tcaatttagt atgatgtttt ctcatgtgca gctggtaacc gtaacctcag
    23701 ttgtcgttgg atttactgga ataactttgt agccaaggac caataataat atattttggc
    23761 ataccttcat catcaaaaat cttcatcggt gtttctccac attgctttga aggaaaagta
    23821 tggcctgatg atgagatagt taacgctgta acaacaccaa ttccggctat aactgctgca
    23881 agaataaatc ccacaacaat tattacaccc gtagtcctgt tatatctgaa ctgcaacaat
    23941 tatatcacca gaatggtgta aaggggattt gaaacgctct gggtacgaag aatggattta
    24001 tggagcatta aaaaaattga ataataacaa caagaaccaa acatgcgtga agtggggtaa
    24061 aggcgtagaa ggtcatgact attttgctag tctatatatt tgtggcaata gcaaatctga
    24121 aataacaaga ggcttaaata aagaaaatac tataggagga aatgagaaat gaccagtggt
    24181 agtttcttgg cagcagtcat aataatattt accttcttag ttacagcatc aataacaatg
    24241 gtgtacgcat catcttcaaa tatcgaaacg accgattcaa aatcgtcatc atctacactt
    24301 tcaagcagct cctcgcacag taagcctgct aacataacaa cacaagaatc taaatccaat
    24361 tcatcagcag actacatgac aggcaataag gcaggaaaaa ttgatgggag cgtaggaatc
    24421 agggatgctg gagcagcgtg tgctaattta aatggaactc gtctgaatca atgcattcaa
    24481 ggctataaca caggatttaa ctcattatgt gcaactacaa agtttgggtg caacgaatga
    24541 caacagagga gggggacatc atcccacaga aaaagatatt gtggagaaag acatcatcaa
    24601 ggttgtccag tgatcagtac ctatttttgt gttttcgtta cctatggtga tggaaacagc
    24661 acataccacg ctgacattca agcagaaaat cactttagac ttatcagcag agtaatcaca
    24721 tcttaggtca tcttatgtac gaaggcgtag tttctgaacc aaaaacataa agcaaaccat
    24781 cacgagatcc tattagcatc atattacttg caatggctgc tgaagagtca actatactca
    24841 ttgttggaaa tttccaggtt tgtctgcctg tattgatatc tatcccataa atatgggagt
    24901 ccaaggaccc aaaaaacatt acgccagcat attcagatag agatggtgat gaccatattt
    24961 tatcgccagt ttcgaaatcc catatccttg agccgtcttt gacctgaaaa cagtatattc
    25021 tcttatctga tgcagcaaag aagaccttat cttttattgt agcaggagaa gccataacag
    25081 gttttggaat ctgtttactc caccttatca ttccagtaga ttgattcaaa caaaacatcc
    25141 ccccattgta agtacctatg aatactgagg gactttgacc ttcatcaaac gataaacatg
    25201 gtgatgagga tctaatcttg ccattcaatt ttgttttcca ccgcaatttc ccatctatat
    25261 ctaaacaata aaggaaacca tcatcagatc ctatgaaaat ctcatttcca ttatatgatg
    25321 gagaggacca gacttcgcca ccggtttcga acttccaccg aacattccca ctgtatcctt
    25381 ctagagcgta gataccatta ttcatagaac cgacaactat tatcgcatca tcaaccagac
    25441 atggcgaaga gaaaacgtca cctgggattt catatcccca aatctgcttc ccatcctctg
    25501 tattaattcc aagcacaaaa tttttccctg taaaagacgc atctttaatc caagagtcat
    25561 atgtagcagc tactaaaatg ctattgtgaa gtaagggtga cgagactaaa ggactgccta
    25621 cattcaaatg ccattttatt tgcttctcgg atggatttaa tgcaaaaatt cgtcctgtta
    25681 tagttgaaac gtagattgtc ccatctccaa atataggaga agacacgctt ggtccgactt
    25741 ctgttaccca taaaagcgaa ggtttgcgag gtatcttaga cgcagaggct cctgttcgca
    25801 tcgaacttcc tctgaacatc gtccaagagt gtttatcgga tattcccaat ggctgtttcc
    25861 cgatcaatac tcttcattct caatatcata tgaaagacat atacttttac acttcttatt
    25921 gccacttgaa ttctatcctc tctttcttct gcaccttcag ttgaagaaga gaggtatcta
    25981 attataggaa tagtataata tcaaatattc aacaaccggg ttgactattt cgagtctatc
    26041 gagaagaagg aactcctatt catccttgtt ggtttagctc aagtttatct ctgacttcct
    26101 tagctctatt cctgacagtg acttctgtta caccggccgc ccttgcaata tctgattgag
    26161 tgataatctc tccagtctta atacttgata ggtagagtat agatgctgcg aaactatact
    26221 agcgaaatta gatgtaatat taaagtcata cggatattta tacctcctta cgtcttaatt
    26281 ataattcgtg agcagctgta ccacaaaaga atgaacgccc ttgtaacaca tttgaaaata
    26341 atagtgaagc ttttatgtac tatggctaga tctattctaa cttactaacc tttgcatgtt
    26401 agcgatattt cctaaatttg atatttatgg cgataatgga ctacgcgtcc cggtaggaag
    26461 aaattttagg tatgtcagca tgactttgat aatatggtga aggcaaagat gaagaatgcc
    26521 aatattgcga gtattttgcg tgatattgca tttctgctag aaatggagga gggagaggat
    26581 aactctaata tagtttttaa aattagatct tataagaggg catcagatgt aatcgcaaat
    26641 ttatcttcaa acgtcgagga agtatacgca acgcacggat tgaaaggact gatgcaaatt
    26701 ccgtccgtcg gaaaggcaat tgctttaaaa attgaagaat accttacaac aggaaaaatc
    26761 caatatttag acgagttaaa atccaagact gatatagata ttgatggatt ttatggttta
    26821 gaaggcatag gtcctaaaac gattaagatt gtatatgaca agctaggtat cagagattta
    26881 tcaggtttag aaaaagcagc atcgggagga aagcttcgta gcattcgtgg tttttctgaa
    26941 aaaaaagaag aagtaatttt gaaaaaaatt caactattta agaaaggaag aagtcggtat
    27001 cttttaggcg aggtttatcc tctaataaaa caaattgaaa cacgtctctt gaagtttaac
    27061 gaagtaaaaa atgcagttgt tgccggttct tttcggagaa tgaaagaaac tgtaggtgat
    27121 attgatttcg tcgtagcttg taatgacgtt gaaaaggtaa tggattattt tgtatcaatg
    27181 ccggaaatag atgaggttct tggtaaagga tcaaccaaga ccttcgtccg cttaaataac
    27241 ggaatggatg ctgatctatt ggtcgttcct gaagaaagtt ttggctcagc attgcaatat
    27301 tttacaggca gcaaagaaca tggtgttgct atgcgtaaga tagcactcgc caaaggcctt
    27361 cacttgaatg aatgggggat ttttgacaat tctcagcaca gaattgctgg ttcaacagaa
    27421 gaagaagtat accagacact ggatttggaa tggattcccc ctgaaatgag ggaaaacttg
    27481 ggagaagttg agctagcaaa aaagggagcc aagggactac cgaaactaat agaatacaat
    27541 gatttgaaag gtgatttgca agtccacagc aatagcacag atggaacaat gtcaattgaa
    27601 gagatggctt tagccgccaa tgaaaaattc ggacttcaat atatagtcat aacagatcac
    27661 acaaaaagtt tgaaattaac aaacggactt gatgaaaaac aactattaga tcaagcaaac
    27721 agaattgcag aagtaaatga tcgtataagg cgtgtgccgg ataaaacaga aaggcaactt
    27781 attaatgaag ttgatttatc aagatcaact cctcgaacaa aatctaataa tttccgaatt
    27841 ttatctggtg ctgaagtgaa tataatgaga gatggatcac ttgatatcgc aaataatgta
    27901 ctagataaac tcgacatcgt aggggcagct attcattcta atttctcaca gccaattgaa
    27961 gtccaaactg ataggctaat aaaagcagca cggaatccta gcgtcgatat aatattccat
    28021 cctacaggca ggcgtataaa taaaagagaa ggatatcctg tcaatattga gaaactggta
    28081 gatattgcaa atgatactaa tactatactg gaaattgatg cacattatga ccgtcttgat
    28141 ctaaaagatg aatatgtaag aatggctata caaaagggtg ttaaacttgt aattgattca
    28201 gatgcacatc atcctgttca ttttggattt ttaaaatttg ggataggtca agctaggcgt
    28261 ggctgggcag aaaaatctag catactaaat acgatgcccg ttgatgagtt gctgaggtgc
    28321 ctaaagtgag gagtccttct gtctccgcaa aaaagatgag aatagaaacc atcaactata
    28381 tcgtctagtc attgttacca aaggcctgat tgtatttcat gtgacggata ctgtactcat
    28441 acggtcgcta acacgtgccg taagttctat gggctataaa gtttcgtgaa tttgtaaagt
    28501 gagcatgtgg catagacttt acttgctccc caacaccatt aaacgaaaac gttgtcgatt
    28561 atcatgccta ccaacacagg tgcaccctta aaggatgcaa ttgtaggatg gagtatatcc
    28621 aaatgaaaca cgaatatctg agctgtataa ttcagatatc ttcgacttta tcttatattt
    28681 gcgctctttt atttaagacg taacttgacg agtggcttta actctaccat atggtttggg
    28741 tcatggcttt tgacctttct ctcttatact gactcaagag taattacggt tgtagagctt
    28801 agctatcggt gagggcgact agtattttgc cacaaatatg acagtaacaa gcgccgcaag
    28861 taatacaaca gatagcaaaa tgatctgctc ctttcactct tctgggagca aaatgatgtt
    28921 cgtgtatcag ctcatgttca gattgtattc tatcacactc aacaacggcg ccttcatgag
    28981 tcatactaca cctgaccgat tcgatttgtt ccacgaattg actatttcca agtttgatag
    29041 attaattcta cttcgcaaat tgtttgatta ctcatcagcg gtctaaacta atcagcaacc
    29101 gtggttatga acccatacgt cgtagctcct ctgcaaagga gagtcactac agcgccgacc
    29161 ggacattagc tattgtcaac cgtttttctc ataaaataac caacaataag cctccccctt
    29221 tgaattattt ttgaatgact caatagttag ctcatttatc acgctcaaaa ctatcgtatt
    29281 ttgaagtcct tctccttccg attattcagc tcatcgttct ttatatttag gtactttaat
    29341 gtcttcaaag ccttctgggt caactatctt tgctaatact tctaaccctg ttattgtttt
    29401 aggtccaggt ttactaaaat acgctcctgt attaactgca tacatttcat tgttctgaac
    29461 agccaaataa aactggaaat ttagaaccag tatttagagc gagcaattat ggcggaaagg
    29521 catttggtcc gatagtgaag ttaaacagca caagcttgaa ataattattt tattttatat
    29581 attattcaga tggtttcact tcctatgccc tgcagccagt caggattaga tcgatactta
    29641 atgttgttgt actgacagaa aaaatgtaga gaatatgcat cttgctttcc ttcaagatca
    29701 tttgttatgt tatatcggct gaattgctcc ttggttaatg catacattac tgctagttgt
    29761 actagtagtc agtggcacac ctgtacagtt gttcaggtgt acaaagaaga gaagttactc
    29821 ccttgaagag tcctacaact gaataaataa tagaaatatt gataaatcaa gcactcattg
    29881 ttgtcaatat atgacaacaa caacgaaatt gcaaaaaaag agtatgaaat caccagatga
    29941 aacacgctct ttcgataagg gcaaagttga gatcgcaaag gttggtgaga ctagtatagg
    30001 tagagcatat tttgaacctg gatggagttg ggagaaatgc gtgaagccta ttgttaaaac
    30061 tgaaagctgt caagctccgc atacacaata tgttatttct ggaagaatga gagttaaaat
    30121 ggatgatggc tctgaagaag aattcggtcc tggagatgta ggttatcttc cacccggaca
    30181 taatgcatgg ataattggaa acgaaccctt cgttgcagta gacttcacag gtatgaaaga
    30241 atacgctaag aggtaattaa gaaagggaac ggatagaaca ggcaacgtaa agaatcaaat
    30301 aatgatggtt aatttactat cctaatagtt tattttttct ttggatttgg gatagctaca
    30361 ccttgtttgt tgtagcttag tgcagtattt gttgttacag ccggattttt gaaccttaat
    30421 atgttcgtta tttgctagtc ggttacacac tatcttcttt gggcgataga tcaactaatc
    30481 aattagcgta tataagataa ataacataat gtattttaac gaaatgctgt gcgtactgat
    30541 attagagatt agtgagcctg gttgttgttt gcgaccttaa tattgccaaa cgctatattg
    30601 cactctgaaa tctcatagag tgtgactgct tgtgaatcaa gctcgtaccg atacaggaat
    30661 ggtagtttct atctggcgtt atcccgtgaa gtcaatgttg ggagaggagc tcaattcttc
    30721 gtacgtaacg gaacgcggac tgatagggga tcgtgcatac gcactaatcg accagaaaac
    30781 tggtaaggtg gctagtgcca agaaccctaa gaaatgggga aagctcttcg actttcgttc
    30841 tgtctttata gacccaccac aagtagtcga gaacattcca tcagtcagga ttaccttccc
    30901 agatggcacc cacattttct ccgatcagga taatattgat tacactttgt caaaggttct
    30961 tgatcaagat gttaggctca tgagggctag tttggagagg ccaagctacg aggagtactg
    31021 gccggatata gatggcctag cacaaagagc gaaggtcacc gacgaaacca tgccctccca
    31081 aactttcttc gatcttgctg taattcatct cttgacgact tctacaatca accgtctacg
    31141 agaactctat ccagaaggac gcttcgaggt tcgacggttt aggccaaaca tagtgataga
    31201 gtcagcgtct ggagagaagg actttatcga gaatttatgg atgggcaaga agataatgat
    31261 cggcgaggat attgtgctgc gtgtgactgg atcttgcaca aggtgtgtga tgacgactct
    31321 gccgcagggc gaccttccca aagatttagg tatcttgcgc acgatcgcaa ggtacaacaa
    31381 ggtgaatgcc ggagtctatg catcagttca gcggggtgga acaattcatc gcggagacct
    31441 cgtgcgattc gaaggataga ctctgcgtgc ttcaagccac taaagaagga aaatcttagt
    31501 gcttgccaat agggctgatt ttaagtctta gaaacaattt tggtaactta taggaggttg
    31561 atagaaaatc tccccggtac tatcaccggc gcaaggttac cagtacccaa aacattcttt
    31621 tttctcaatt ttcataagaa cattcaatac taccgattta tgatgtgtca tatacatgat
    31681 ttcttaggct tggaaatatg aagtgttgtt tctaatatct atctcccaca gaaatgatat
    31741 tataaacact gaataaaatg acagaatata catagtttag tgtatactca tccctg
//
LOCUS       DAOR01000052            5609 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_24382, whole genome shotgun sequence.
ACCESSION   DAOR01000052 DAOR01000000
VERSION     DAOR01000052.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_24382"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4186..4381
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1689 a         1057 c          991 g         1676 t
ORIGIN      
        1 cctctatgcc tggctctaac tcctctatgt ctccagctgg tgctgctggt actaatgcat
       61 catctgtttc aattgtcaaa ggcgcttcga atcctagcac ccaaaaacct tacaatcctt
      121 caccacttaa tgttgcagta ggcagaactg ttttatggat aaataatgat aatactgctc
      181 acacagtgac tgagggaaat ccttccggta atacaccatc aaacggattt gattctggaa
      241 tacttagtcc tggacagaca ttcaagcatg cttttgataa accaggaaca gttggctact
      301 tttgcaggct tcacccattc atgttgggac aagtaatagt aaagtgaggt agcttgaata
      361 cttgtttgaa agaccctttg gttctacgtc cagtgggtaa tgatacactt cccatcgaca
      421 gcttgtccgg tgtgtgcgtg agtaaaattt attttattaa tcgcattgtt caaagaattg
      481 ataatagctg gtgttggact gctaacagaa ttcaactgat tcctatttcc tttggaactt
      541 gtttgtacta ttgtcgtatg atcatacaaa caggttgcgc ttaagaatgt aggaaattta
      601 gcaagtgcga tcgtaaacct ttagtaatca tcaagaatct ttgcacatac aagatacatt
      661 ctgtaaacat gttttgtctt ttattcctag tcgtagtgat ttttgttaat ttcatacgcc
      721 ttataataaa atcgtctcta tgattccact tgggaagagc aggcggatcg ctccggaatt
      781 tccatagata gataatcaaa gatcccaagc tctcgaccaa gtttgttccg gtcttcaaga
      841 acccaagaaa gctttagaag atgccgctgc aaagtctgca aaagtgttgg gatgatatct
      901 cgccagttga acgaaacgtc attttggggc atatttcctc cgttgtctga attcttatat
      961 gctttgttag ggaattcatc attgtctaac atttgcggac cttcaaagat accttcgttg
     1021 tgaattgtca tatagataga gtttgggaat tttttacaga tatcaaacat ctcgaaataa
     1081 taaccccaaa ggaaatagaa ctcaaaatta ctaatgtcac aaaccaaaag cttactcaag
     1141 gatcagaaat ttgggtagaa ggtaaaataa caatgatgct ttccaaaaga agtaaatggc
     1201 attctatgat atcttcgtct tcagtatctc caacgcaacg tcagtatgta gatgaaatgc
     1261 taactggtcc attcgaaaaa tggagacatt tgcacaaatt ctatgatgtc aataacaata
     1321 acaaccaaaa gcaaacgcag gtggtagata ggattgattt tgaactacag tatggcagaa
     1381 taggcaggtt atttgatggt tatgcatata aaagtcttca gaaactcttt tgtcacagaa
     1441 aaatagctac aattagagct ctagaaaata acatatagac actaatgttc ttccttatgc
     1501 tcgttaaaag gtaatgataa ttgaatactt tgaaataaca ttcaaaagaa tttaaattgc
     1561 gtttttatat tacgctctat aaatactatc tatctatttt ggactcgtca acaaaaatgc
     1621 atctacaaca acagtattat cggaatttcc tagagtcaat aagaacgcag gcagcaagac
     1681 aaacatatga ctttcatttt aagaaattta ctcaattcat agaaggtaca gaaggaaatc
     1741 tactaaatga aaatcccaga gtaatagaat cccgaataat tgactatatc atttacctga
     1801 aaaacaaagg aagatcacgt tctcttgtct ctacagctgt ttgtgcaatc tcacattttt
     1861 atacgatgaa tgacgttgtt ataaacaaaa agaaaataaa tacatttgtg ggagcaagga
     1921 caaacaaaca caaagacata ggatataaca caaatcaaat ccgtagatta ttagaagtct
     1981 gtgacgatag aataaaggtg atggtcttat tgtttgcatc cacaggtatg agattaggag
     2041 cactaccaac cttgaaaatg agaaattgta gatctgtaaa tattgaaaat gatagacaga
     2101 tcaaattata tcagattaca atctatgagg gcgagccaga agagtaccaa ctagatgtat
     2161 tttccttatt aaatctgagt cgttgcaagg tttgcagcta tattctggca tattatgcaa
     2221 tggtgcactg cgtcgcgctc aaaaaaagtc ttaaaaagat aaagtcacta tcgtttttga
     2281 tcgaaatggg tcgaataaca tatgtctgcg caacatgttc agagcacttt actaggaaat
     2341 acagtgcgac aagacataat cttactttac acaacggtag atgggagatc gttcgactac
     2401 ttgaatatct agagggcaca ttatcaggcc gatatctgcc cagccatcct tcatggtata
     2461 tacgtagcca aaaatgggag actcccacgc tcataactct aacaataaat ctggacttgc
     2521 cacctttgca gactctgtag gcgaaacgtt caggcctgga aatgccctgc aacaagcacc
     2581 ccaaggtaat acgtacaaaa aatcccacta ttctactagt ccaatactgc aagctatgca
     2641 taaaacagat gatcaaccat atggaactca atctcaagaa acgagcctaa aaattaaaga
     2701 actcgaaaca ctcctgaata agcaccactt tccgcacggg gatacaattt taaggtgggc
     2761 tgtttatgcg tcgacgaaag gcaacgggaa ctttgttgat gaaaggttgg ccccgcttcg
     2821 caagtttgac acacttcaga gctaacattc ccattctgtt gggatttcta taacagatag
     2881 acatacttca aacacagttg aacaagaaaa ggaagaaaga ttatctttta caacgtgggt
     2941 ttttggagga aatgagagag gagactggag ggtgtgaagg gagtgtccga aagcaaactt
     3001 ctgttgtatg tgaaatatgc ggtggtgctt tctgtgatga tcacattttc aaggtaaata
     3061 attcacctca tcattgattt agtatggaat gtttcgatat ctcctcagct acttctttct
     3121 catttttata tcgttctgta ttgatgcata tttctttaac ttgacctttt acagcaaaaa
     3181 tggttagcag cttttgataa aatgtattaa aagaaagatc tatcttatca tcagtaggaa
     3241 tatcatgact tctgttctta actctcatat gacacgtctc caacgttgat agtaatataa
     3301 ctgacagtat ggtgcagcct ttatccttaa acttgtgtat ccatgtttga ggatctgttg
     3361 tgtgcgacgt tccttcagat atttctacaa ctacattttc ataattcagt gcccgctgaa
     3421 tatcctgcgc acattcttct cctgttctat ccaggtctag tacgactgta ttattatcgc
     3481 caccgacaat ttgtttgatt tgtgttgcta tagatgattt gcctgagccc atggggcctc
     3541 tgagaataat caaataagcc atggaaataa tttagtgatg ttggctattg ttaaacattc
     3601 tgcatgcaag ccttacaccg tacttcttac tatcatgtcc gtaacaacat caagttgtac
     3661 atggtgcgac atgtacaaca tcaagtcgca agtttatgcg atcaactttt ttcgtaagcc
     3721 tgttttatga gtgtcaagac ataggaaatt tcacctaggt cagtaattgt cacctcggta
     3781 ttgcaatgtg agtaatgccc tacttctgta acgtcccttg ttttctttaa tggatcttga
     3841 atatcggaaa aatcaatgtt gaggtaagcc ttaagtttag actttagaaa cacgaagcca
     3901 acaaatcttg cttgcgtcga aaagcaatat agaatttctt tggtcttatt tttatatctt
     3961 tacataaaca atcgaactaa gaatggattg tagagacttc acaagggcca gcaacacttc
     4021 cgttagtcca gtttctcatt ttgtttcgtc tactatatgc ataccaagtg attttcaatt
     4081 aaaccaattg ctgtcggtgg ttctgattgt gctcacataa aactaagctt gatggaaccc
     4141 aaattaaggt ttgtagtggg cacgtttaag gtaccgaatt tgctannnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nacgtttaag gtaccgaatt tgctagcgct ttggagtaga tatctgcaag ccgtaacggt
     4441 tcgggaattc gttctttcgg cttaactaat tgcttaacga tactgatagc atttattaaa
     4501 ttaattttgt gaccgacact tatgtagatt ggtttctttt cttcctttat cattttaaac
     4561 ccattgataa ctccatcaac atcgataaaa tgatctgcct taactgagcc gcacaggatg
     4621 ttttttgcaa caccaattgt tggactgtct gttataacgc ctatgtaaga ggcgagcctg
     4681 catctcctag gatgaagaac gccatgacca tcaattaata gaaggtcaaa acggtttttt
     4741 aacggtttca aaatatttat tattacatct gattctctta acataaaaaa actgggcaca
     4801 tacgagtgct taccttcaat gttatgttga cagattcctt tacctccata gtattcttgt
     4861 tcatcagtac cgcagagcag tacgcaatgt tattcctgta cgctacgttc acaccattgg
     4921 aaatatacaa tcccaatcct gtttcctgat actcttattg attaatggct tacagtccgt
     4981 ttactactca tagtgaagag caggtagcaa ctcgtaattt tctttgttat ttatattggg
     5041 aatcacggaa ccgcccagaa gtcctgaaaa cggtatgaca cctagggaca tttatgatgc
     5101 aacaacacaa taagggtcca atatgtagac cgacagagag ttagaaaaat agccatacca
     5161 catgttgtct agttgataaa gaagaatatg atgataacga accagtccta aacacaaatc
     5221 aaccgttttc taatataaaa agtgtcttgg atcattccag atttttgacc catcccggtg
     5281 ttactggtca ttccaccagt tgtagtactg cccctaccat tgtcagacat cgttgttccg
     5341 gccttccgag tcatttggct agtagtacct tgcatcatac caccagtacc actactatta
     5401 cttgacatca ttccagattt ttcacccatc ccagtatttc tgctcatagc ccacagaaag
     5461 cccattttga ggatcgtcac acggtaactt ctatttgtct tgcaccaact tggttaacca
     5521 tgtaaaaatc attggctatt tgaattatat ctcctgcagc tattctgcta tgacttcttt
     5581 tttcatttac tggtattcca aagggtctt
//
LOCUS       DAOR01000053           25706 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_50694, whole genome shotgun sequence.
ACCESSION   DAOR01000053 DAOR01000000
VERSION     DAOR01000053.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 25706)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25706)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25706
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_50694"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    13292..13428
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13923..14179
                     /estimated_length=257
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14313..14615
                     /estimated_length=303
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7981 a         4586 c         4735 g         7707 t
ORIGIN      
        1 cccgatctca tctttatctt agacttcaac aaaaatatct tcactctata tactactaaa
       61 acaagtagcc aatccggata ttccttttgg atagtttctt tgattattgg tactgactac
      121 aatcgcttgt ttcaatagat atcgagcctt attcagcccg tgttcatttg atgccaactt
      181 ttttcaaacc tgttaatgcc ttgaatttaa taattggtgg atacttggaa ggagtcctca
      241 tatatactag tttgaagttg aagttgataa tcagtattag attaaaatcg ttaacgaaat
      301 ctaaacgtat aatctagatc catcacaatc gaataccggc tacaaagtcg tggcaacgat
      361 acacatctga cacaatgttg aatcatgaac tcttgatgca agttatgaga gctattgaat
      421 tagccctcat atggactcgc tccaggtctc ggatcaaaca attcaaggta cgagtgctaa
      481 agaaattagt cttttccacg atctctcttg gaagatctca ggagtatatc cacccatgtt
      541 taccttcttt gaaactctct ttgtcagata aagagcgaac gatcctaatg tgtaccaaaa
      601 tctgctgtta ggattggaag taaaagaagt caaggcccat ttaaatagat gataagtctt
      661 agggtggtat tccaacacat aattcattaa ggcttcacta gagctggaga gaacatagta
      721 agcatgttca taaagctcct ttttagttgc ataatttgcg ctatggatct tggatcttcc
      781 cttaagcaga ttgtcaacaa cagaatcgat gttgttctcg gattcaattg cgtttcgaca
      841 tctacccacg gtagaacaga catgagaatc gcttcctgca attgtgaaca ttttgtgata
      901 ttctgcgaat ttgcttgcaa ggatattgga aaagatgtcc acgttgttgc tattaaagga
      961 ctccattaga tcgcataggc tcgcttttcc ccggatacca tttgaaactg caaaaggatg
     1021 agctgcgcat gatactgcgt tttgtcgttt tatttcgtct agcgtttcct ctagagtcat
     1081 tcccggcgta acagttttgt ttatgccgta tgcaagtaca tgccctccat tatctactgt
     1141 aatttcttcc gcaggataaa tcctaatatc ataatatttt ctatgatttt gttggtactc
     1201 aagtatctca tgatagccct taagcgtatt atggttagtt acaaaaagca cgtcaatatt
     1261 ttgttttagt gcgctctcta acaattgttc catacttata ccgcaatcaa atggaatacg
     1321 atctgactgg ttcctagaat ttgaatatat attatgacaa tgaagctcta cattgagtaa
     1381 atctgtcgtg gcgcttatac ctactacaat aggatatagt caacagtgtt taattaggtt
     1441 atcgcaacat cgaatcgaga agcaattatt tgaaaggtaa tcagcagaag atggctaaaa
     1501 tcaatatagt aatagacgat gagagtgtag caagatttgg agggttgcat gtaaagagat
     1561 actatgcaga tctaatagta atgaccgata cagatatcat aaagaatgat cttgatgcgg
     1621 gagataatca gagtgggatc acttcaatat tcaatggccg cagaaggaag gtttatctaa
     1681 attaaagaac atgcaattct ctcagaaaga ggctttatcg ctgccaccta aattttagaa
     1741 gcttcggtag tcatcgtccg ttcggagaga tcaagtaatc aagtacatat ggaatgctgt
     1801 tcagctgatg acataaaaag atgcttctat tatatgcaca aagtcagata tcatagatct
     1861 agtaaatgat acgaatatga agaagattaa attttaatat gaaatatggc aaatattcgc
     1921 ataaactggg tacctactaa aacaacacga tagatactcg ctagggactg cgatatccaa
     1981 agcgtcataa agttaataat gaatccttgg catataccta gaagcgtatc tttgaaggcc
     2041 atatatacta agaaagtcgc atccacaagg agaacatcta gtgtaaaaaa cgaaaggaga
     2101 aagcaagatg aagaggagct ttacatagga accgctgaat ctgagcatat agaaatgtat
     2161 ctgaaagcct tatggtatat tgttgaaaaa ggcgaagaac ccaaaataag ctcgattgcc
     2221 aaattactca acattagaca accatctgtc gtcgaaatgc tccgcaagct aaacgaagcc
     2281 aacttggtag aatatgagag aggcagtata gtaaggatta cttctcaagg caacaacatc
     2341 ggaagacaaa tgattcgtaa tacaaggttg ctcgaagtaa tgatgaaata tgcgctaaag
     2401 atcgatatag atgaggaaat ggcttgtggc atagagcatc acatgaataa cgaatttacc
     2461 gacgctttat gcacattatt gaaacaccca aggaaatgtc cccacggtca taatattcct
     2521 gcgggaaaat gttgtaatag gagtagttag ataaatataa gcttcaatat cataatttag
     2581 atttttcgaa gtgagatcat gatccagtaa aaatgtttaa atagcgtgtt aggaagacct
     2641 aactaggtta gcctaatatg aatagactcg gcgacattcc tgtatttcgc tcccctattg
     2701 gtcttcgagg ctaccgcgat ctcacgaata ttgtacctta ctttaatggc ataaaaaaag
     2761 gaatggtacg acaagagacg cgttatacgt gagtatggtg aagtgatagt atgatggagt
     2821 attggcaact tttgcttcta ggatccatag ctggttttac aatattcctt ggattacctt
     2881 tggctgtact gcagaatgta aacccaaaaa agaaaggatt tctcaatgcg tttgcaatag
     2941 gaattttggt atttcttata atagatgtat ttggtcatgc ttggcaatca gttggaatcg
     3001 ctgcacaagg cgcttttgca ggtaaatctc ctttgggaaa tgcagtattt gatcttcttg
     3061 caatgtttgg agggcttgca ataggacttc tgggtctcgt cttttatgag agtagataca
     3121 tgatgtctaa gagtggtcat aattcaacag caacgatgaa atcattcctg cgaaaccgac
     3181 aagaagcatc agcaaaggat gtggtcaaag atggtagtgg caataaagct agtgacggta
     3241 gtcaacaaga acaacactta ctgttgcaag agataaatgc atacaaatta tccacaatga
     3301 tcgcaatagg cataggcgca cataacttta gtgaagggct tgcaataggc caatcctaca
     3361 tatcgggtgc aataggatta gcaatactct tgataattgg atttggatct cataacgcaa
     3421 cagagggttt tggaattgcc ggcccattga caggtctcgt gagaaaacca agaattcgct
     3481 tcttaattat ggccggctta attggtggtg gccctacatt tgttgggacc attgtcggta
     3541 gcttctggaa ttctacgata gcttatgtgt tattcttgtc tatggcaggc ggagcattag
     3601 tctatgtttc aatgttgatg tacaattctg gtagacgaca gactacaaac aatatcatga
     3661 tgatgggtat atttgtcgga ttgtgtgctg gttttgtgac tgatttatta gttacacttg
     3721 cgggagcatg agaataggta attcgcaaag atttagagta gaaaggtgct ctatgcgggc
     3781 tacgtacgat attcaagcgc attagggtca tttatggcaa ggtggctagc agtatttacc
     3841 attgcaggaa ttcgatcaac aacagctaaa ttatatgcac aacaaactac gataacgtga
     3901 gctagtcttg gcacagtctt catgcattgc tttattaata ttgtcgtggg agagaagtag
     3961 atgtacgtcg tacctaaccc atcttctcgc ggtcaacatg ttatgctttt gtatgatatc
     4021 aattctaacc gcgaccttgc cgcaataaac tacatcaatc aaggactagc agaaaagcag
     4081 ctttgtatct atgctactgt taatgcctat gacacatcac acttatcgaa gatttcttct
     4141 cggattaaaa attacgaaga aaacatcgat aaaagaaact tgttcatcgt aaatctgaaa
     4201 ccattttatg attctgtttt agcaggcgat ctaacaccat tcgaggagtt caaagtacaa
     4261 ttacaacaag agctaaaaga cagagacgta cttattgttg ctgattgtgc tgataacctt
     4321 tttaggaata aacactttga ccagtgcgag ctggttgaga agtggtggca agagaattat
     4381 atagaatggc gaagactacg acaaaatcat attactatca tatgtcctca ttcaggctca
     4441 ttactaagca agcatccatt tgaccaccac aagcatcaga tttctcataa ccataccgtg
     4501 actatagata caaccaatcg tagaatgaca ggatacaaaa cggccaagga agttgcagag
     4561 tcagccattt atccactaga aatcccaatc cagattctcg tcgcagaacc agaacgagat
     4621 cttcaacaga tatacagtat ttggctacgt tcaatgggct tcaaaaacat ggcaattaca
     4681 gatagtggca agaaatgtat agatgagctt ctcaatatca cagacacaag caaaaacaaa
     4741 acccacggat ttgacatgat tattatttta gatacacacc ttaaggacat tccctgcatt
     4801 caggttgcta gagagattgt taataaaaga ccagaccagc gaattatctt tacaactact
     4861 ttgcctccgg atagtgttag acaagacctt tactcaatcg ggatcaagaa taataatgag
     4921 atactgttga agccctttag attttctaag ctattatcgt tgatacgtat gagtattaat
     4981 aatcaacaag atcgagggtg ttaggttgtc tgatcataaa aaaaatgttc aaaacgttgc
     5041 attgtgtgct gactaacaaa ttggctaatc ctgattcctt aaaagagtga ctgcaatgca
     5101 cacctattgg aatagatcta cccaacccca cataatagag gtatatctaa gccgccctta
     5161 agactaattg gaatttaata ctttttaaaa atatgtcatt tatggaattt tagagtcttg
     5221 gaaaaagata aatctgttcc ggtttacgcc aaaggcagtt cgaagacttt gaaatacttc
     5281 gagtagacag aaaaggacag gtatgacatg gtcttcaact gatatcgcaa ttgttaccgg
     5341 atacaccggt gaggtaggat tgcttgatga tttgtgagtg gtagtatgat tggcaataac
     5401 acgattcccg gggtaaagaa aacttgaata taatacgtct agcattagaa agcgatggaa
     5461 attgatggtg tgttttgatt tatttttttc gttagattgc tcaatcggag ttgatagctt
     5521 aatgttaaaa tagctgattc ttgtgatagc aaaactatga cgcagttgat gcttagatgt
     5581 aggaattgtg gctccgaatt tctctcgctg atacagatgg acgaagaatc ctttcattcg
     5641 gccatcctca gcaatgagtc tgaaccgtgt cctaactgta cacaaacatt tgcccacaat
     5701 aagcttgatt atttcttcaa ataaccacat ttgtccagca actctccttc cagtttgcca
     5761 gccaattgtc ctccttcctt ggcctttttt gtttacttaa gcaactaatc agtctaatcc
     5821 atctgaatgt agaaatccaa gatctttttt gcggagtctc atggagcgcc ggtgcatgaa
     5881 aaccctgtat acaacgactc atcttttgtg gatggctaat atccattgct atcagattgt
     5941 caaacgggac gtttcgtttt aaaggaactg taaagtgaat atgtataata tatcgcgtga
     6001 ctagttataa ctcagatgca gaacaaacta gcacacacaa gtgagcacgc atttataggc
     6061 tcacttcaga agctaatggg taaaacactt aatgtccgga aagtggaaca tagggattac
     6121 gataatctag tgcttattac tgctaaagac attgaagtgg atagtgttat agaggcagaa
     6181 agggaagtta attcgtcaag ggagaagagt tattatccac tctttcggat cactggctga
     6241 ggcaaagagt catctaccaa accttagagc aaatgaagac cgtattggag ggaataatga
     6301 ggttagagtt gttgagatcg aaggtcatga cttggcggca tgcgtgatgg atcatgctgc
     6361 taatctcgaa gaatgcggtt tcttcctgat tacacggatg aataggatag gttccgacta
     6421 tgaaataaat tttgtggttt ccgatgctgc caatagccac gctatagatc tttcctccaa
     6481 gatattgaag atttgtacag agacaggagc aaattacaat acagtcgatg agacagtcaa
     6541 aaaattgaac caaactaaca tcttacatct caagaaatta aggaaactaa cagaagaagt
     6601 tcttaataat atcgaaccgg agccgacacg taacacaggg ccttgcatca ttagtggcac
     6661 atttactgga ttattagacg aacaagttag ggaatttgca agcagaaaga ttagtgagtc
     6721 taatttagtt gtcgttctga ctaatctaaa ctcggaacta gattcaatgg caaacgtagt
     6781 atttgctagg agtgaatcac tctttaacat agattgtaat agaatgttca aagaaatagc
     6841 gtccgaatgt gggagaggag gaggcaaatc gaactttgtc actggcgtga tcagaagaga
     6901 gaatgtggga gaattcatca gctctaatgt taggatggtt gtaactaagt gatatcgggc
     6961 gttaacccca tttcatttgc ttcttaaagg aagtgttatt ttatatgctg gaaagctcaa
     7021 atacatatcg acgtatcatt aagataaatg cctgaaattt cgcatccatt gaataacatt
     7081 cgaaccaata aaaccgttat ttatcataac taagttatta tctgcaggaa aatacgaatc
     7141 ataataattc agaaattatc ctagcagtga cctctgcacc tcttgaattt aaaattggat
     7201 tttgatggct caattccaat ttttcttcaa ttagccgttc cagtttagat atgtcttcat
     7261 acttatatcc caacctaagt gcgttttgtt cctgttcaaa atgatttttt attggaatga
     7321 aaattcctgg tgttccatat acggttgact cgtctatggt cgacctgccg gccaaggaaa
     7381 ttaccagatc ggacgcatag acatactcat gcaagttgtc gacaaagccg aggttgtaaa
     7441 aatcattaga atcatctaat tttaggttgg gacctgagac aagtataagg tctacatcaa
     7501 atctcttttt caattttcta tacgcttcaa gtgacttttg tataaggtgc cttcccgcat
     7561 cggttcctcc tgcgcaaacc aaaattgtct ttctagttaa accaaagtta tatcttaatg
     7621 agttcctctc tgatttgact tctctaacta tcgggccaac atagactaaa ttagctttat
     7681 tatcgccaaa atctggaatg atcaccttat cacatttatt tattaggtga cgcatagacc
     7741 tattcatctt cttttcaatt tcgtgtaaaa agccactcgt gaagtgtgtc tcgcctatat
     7801 tattttcaaa tttaatgtta ccataggtag atgtaatcgc gaggcgagag gctttatcgt
     7861 ttatagtatt ggcggtaggg tctaccatat cataaataag catgtttttt gataaaatgg
     7921 tattctggct aacggcgatc gacgcaaaat cttcatcact cactataaca ggatatttat
     7981 gtgaagattt ccttataact tcctctgcga tttttttgca tcttttatag taaatgatgt
     8041 acttcattag ccatacgaac gggttacgta gcttacctga gtctactgtg aacgaaggtg
     8101 gtctgtaaag gttaagcgct tgaaatcctt cttttgagat tagatcatat gctgccccgc
     8161 cgctgataaa caggatttcg tcatttacaa tctgttttaa tttttcagct atggcaatat
     8221 ctcttgtggc atgacctaat cctatgggac tagtgaaaaa tagtattttt tgattatatg
     8281 ataaggttga caacagtatc tcaactaaac atttggctag tataaatccg actattgaca
     8341 gcgcattaaa cactaaaaat ataaatcgaa tttctgtctc aattatagtt cagatacatc
     8401 aatatgtcct tcttcactgc ctgattttcg gctttttgac attagcgtgc attataacta
     8461 catacctctt ttggtttcac caaaaaatag tgtaacctgg cagcattaaa gagaatcacg
     8521 gatccaatcg cagccattat aatagcataa ttgatgatta ttccataggg ctgcacaaat
     8581 aatggttgga caatcaagaa aacaatagat atacatgcat aactaatgac acttataatg
     8641 tgtactcgta catgaacacg cattttatgt tccaattctc tattccacaa atgccataca
     8701 agagcaaccg catgccaaat agtaattgat aaaataagtg taaagaatct cgtatgtgct
     8761 agaaatgatg tatttaatat tataaacata agtgagaaac gtacaatatc gatgaaaaac
     8821 cttccattgc tgataccata agggtatttg ttgattaatt cttgataaat tacccattgg
     8881 gatactacca aaagaatgcg gttataaaaa ataaaaggtc aaatagtact ggaatatcga
     8941 aatgatatag ctttggctcc tgtggtagaa gaaaatcgaa caagaatcta tttagtccaa
     9001 ttgtgataat gcctccaaag aatacattta ttagcaatac tttggtctgt tttttctatt
     9061 ttagattcat agtagctacc atacccgtta ttatttatct ttccaatcaa actaaccaga
     9121 tagaccattt ctctgttata tgacgaaaat ttagaataca atcaaagctt caagaatact
     9181 aggcacattc taggtaagat gcggaagtta aagacattct aggtaatttc tatagatgta
     9241 tgtgcattaa gatgctacta gttgaagaag cgcaagaaca tatgaagaag gtcctgacac
     9301 tggacttata gggttgaagc tatgacgagt gctaaccaca tagaatatcg atgtggttgt
     9361 agagtgattc atgtcgaatc tattcagggg gatgtgccga ttaacaaagt aacttctctg
     9421 aagctttgta acaaacatca taaaataagg aagcaagaag cctgatgact agtatggatc
     9481 acagcgatac tgatgctgat acatagacca aactacctgt atttgttgtg tcagatctgt
     9541 atttacataa gtagcactcc tattgatcta actcaaggaa tgagagatct ccaccacata
     9601 caggacatac ttagatattg gtagtatctg agtaaatact gaccttgaag aatcagatgt
     9661 tttaaatctt gtacaaaata tgcatgtctt caacgttctc ataacaaagg ttcaaccaaa
     9721 ggcacaatta cattaccatt atcaccacca gaccaagtgt cgtcggttgg gattaaattg
     9781 aagaatcttt tattcagaga taacagttgg cgagacactc atagctatga tctatataca
     9841 tatcgtactt atatgacaaa ttatctgcaa ttgaatgttg ccgactatgt taaacaagac
     9901 catctgattc ttagaagggt cagagagatt tttctgtaaa tataatccac attaagacaa
     9961 tattacttat gatattcttg aattatcctg ttttgtacta agtactgcta tccacagttg
    10021 ttagagtaac ggtttaaatt gagataaaga tttgtcgtca gaaattgcgt gcaaactttc
    10081 agacacagaa accatctttt tggtagatat gttatgcata ataaataata gtggtcataa
    10141 taaatagtac caacagtcat ttttgttgat ataataagat cttccagtgc ttgtaatgta
    10201 cgtatctgat aatagatatc ggttgcaccc tctgatatca agaccactaa aagacattct
    10261 tgtcgcacaa ttgggattta gtaggataaa aactctccat agaaagcatt tgatctaaat
    10321 agaatacata cacccgtgat ctatattcga taattttttg tttttccgca tagttagaag
    10381 tcacacggat aagcggcaca ccaggatgaa gggattcacc taatctattc gtgattcttg
    10441 gtttcctttt tctcttgagc gaattcatct caaaagaatt gaggttggtt catccttagg
    10501 attagtgaga accaatctac ctttgtatgc atttgcaacg atagactggt ctatatcgat
    10561 gagtgttttt ttgattcctc cacagatttt tcggcctggt ttaccaatga taataaatct
    10621 ttgatcatag aagctatgat atacaatctg ttctgttctt attgacatag aatagaagat
    10681 atctctgcaa aagatacgtc aaaataaagc agttcgtctg atagccgttg ttgttgctcg
    10741 agcagttgga cgcattcttt tggttctgtt ttttgagatg ttcaattatg gtgcaggtaa
    10801 cgttgagatt tagtgcatta gcatatagta ataatgaatt ctaattctgt acttctactc
    10861 atgaccgaga gtgaggccat taagatcaga ccatgatggt gtttaaatag acagtagtcg
    10921 atatggagct gctgaattaa acaaagagcg tacaacagaa cgaagataca catccgcaga
    10981 agttgttgaa tattagagta cggaagggaa ctcatgtcag cctaatcgaa caagtggtgg
    11041 gagcgaatca acaggtcctg atcaacacca agtgagacag taagaaacta cctccacatt
    11101 tttggacaaa gtaagggttg gacgaaaaaa attcaagaag agtaaaaaag caaaatgact
    11161 agtaataaca aaaacgataa caacaaagtc aaaatagacg ataagttttc agtctatcag
    11221 tacgatacct cgataaagca tgctcaggac tttcttcgta accacataag ctccaagata
    11281 tcacttctta ttgtgtacgc agacctagta ggctcgacta aaatgagtat gacaatgcca
    11341 gttgataaac tagttactat aatcagagcc ttttcacacg aaatgtcctc tgtggttgaa
    11401 agttatgatg gatatattct gaaatatgtg ggtgatgctg tcattgcttt ctttcctcct
    11461 ggctttaata gataccttac atgtgacaaa gtagttagat gcgcacaatc gatgattaat
    11521 gcgatcaaaa acggaatcaa cccaatatta aagaattatg attaccctga actatcttta
    11581 aaaatcagta ttgtcgaggg agaaattgta gttgttcaac atgcctatga taaaagctca
    11641 caaattgatg tacttggata cgttctgaat gtagcagcaa agataatttc attgaccgac
    11701 tcgaatagac tttcagtagg agaaaatgtc tataaattat tgcatcccgg aatacaggca
    11761 gaatttcatg agttagtata cggaattggt gaatgggatt acattaatcg tgacaccggc
    11821 aagctttaca aagtctacac actgaactaa aaaatagcac taacactagg aagcaagtat
    11881 tattttttct ggtttaccga aataaatcca aaacctacgc ttgcaacttt tcaacctttc
    11941 aactcaaagg cagtactcgt tgtagatata agaggggaag taaaatagca atatcagtat
    12001 atctaattgg aatattttaa acctgcctct aaaccgcgga ttctatcgta tatacttgaa
    12061 tgatcagttt ctacttctgt agtcaaataa agtaaaagat catctccaaa tggcatagta
    12121 atgcgtttga ttttttcata ttcagctaaa acatacattc cttttccgat cttgtctgaa
    12181 agttcactac gtaatttcca tgaagccata gccatctcga gagatttttt gctttcatcc
    12241 ttgctaagta aattctgtac accctgacga tgccgataga atactatatt gccatttgta
    12301 tcacatatag tgactaaccg tatctttgga tcgatattca taatatcttt atagtgttgc
    12361 tttattttat aaactctaat tcttagcaac gagctcatca caaaaccaaa tattgctccc
    12421 caaacaaggt ggaatcctat tgcaataagt gcaatgtttg ctattgaatg gcttagctgg
    12481 tcagccagta tggtcttctg caatgcgatt gctaaaatta caacgatgcg ctgagcggaa
    12541 ggttgtatta aaagtgatgt tatcggaaga aataatatta accatataac aatgcctgct
    12601 cccatgccag ataatgtact tttgaatgga ataagcatgc gtattttctt ccatctaatt
    12661 ccgattgttc caagtgctat tcctaagagt gtgccaatta gtagatgaag tccaaatcct
    12721 atataacctg catttacaat attgttaaac cctaagataa taccaataac tgaataaaat
    12781 gtgcctatct gtaatccaaa tgccagttca gctgctgcaa tagctaaaga tatgagtaaa
    12841 gttgcaataa aaccagcaaa tgcaccatac ttgactcctt ttaaagttac taataagtcg
    12901 tttctgcttt tgttcttctt atgaactgaa gggttatagt tcacctttca actaccaccc
    12961 tctctgtgct gtttatcgtg tgactgctat tactactctt ggccaatgtt ctcactacta
    13021 tacacatgca aggttttatt gtcatatctt tatgaatact gcacagcacc atgtcaactc
    13081 gaatgcgata tagctaatag tgaattccga agaaaaaata cccaacctta tattttgata
    13141 caatattact ttctgatagg aggataaata taaagctcat gttgaaataa tattacattc
    13201 agataataaa tcaattgcaa cattgataaa tgttaattct tttatatgaa aatgaataat
    13261 tccgatacca tctgttaagt taattaattc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt aacaagccag
    13441 gttttataaa taaataataa tgacgctgcc ctaataaaaa ttggcatatt tatcaaacta
    13501 ctttgttcac aatacaaatt taaactcaat atatagcata tgtacttaaa aataacaaaa
    13561 caaaatccaa gttatacaat gtaatatgct ctttttttct gataagtggc aagaagtgca
    13621 ttttcgtacg aggacgaaac ataatttcat ctatttatgg ggcctttatt cctgatgaat
    13681 cttctgctgc ctaatttttg ttcacgacgc taactcatac ttcgtattac tcggctttgt
    13741 tcttacaagt ccacaacaag gatagcagca caacccattt catttaatag agatttcata
    13801 cacaatggat cgtatccgtt ctacagcata aagttcaaaa ctactaggtt ttttcgcatg
    13861 aagaaatcaa tagttatctt tcaatgctag atattgcaat aggcaagact gtatctgtag
    13921 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnc atgaatatat tttgatacac aaataggatg tttaaatgat
    14221 actgattcgt aaattattca ttggtttgac gacacccacc ttgttgaaca tatacattca
    14281 attaggttat aagtcatgat gctatggaat aannnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatatc cttttttctt gttttcgcat
    14641 catgtgtttt tattctcaaa aattcgtggc aacatggaca tcttggacca ttccacttta
    14701 taaaaatctc acaatctgtg catcgcttat aaccatatcc ataatagtta cctccgtacg
    14761 attttgtggc tctgtaaatt gtacaaatat tcctgcatga cattttgttc ttgtattctg
    14821 cttgcttctg acttgttttg ttctttttta tttggcttct acttgaaaag ataagaagcc
    14881 atcacgcctg atgatttctt gtactatctt gtttattggt aagggtaact cacacaaaga
    14941 tgattttcta ggtgactttc tgttctggga gattttattt tctcgtcttt cctttttaaa
    15001 tttttcttca tcggacatct cttcctcctt ttcttatctc attgggcatt gtggaaagag
    15061 tatggttttt tgcctagaac gtacaatgct ttattgtatg ctcatgagta cgatatggtc
    15121 caggctattt ctattgcaga tgctaaatcc tacagttgga tagacgatta gattctcgag
    15181 catagctaat agttgatcag gactgatata aaattcagta caaaaaagat taacatagta
    15241 tcaattatca tatccgtcat ttagtaattg ttaaggatta taatcgattc aggcagccaa
    15301 acaatcagat gtaaactgtg caatagaagc gtgactgcac gtatggccaa ctggtgatat
    15361 agatggagga acaatagagc tagccgaaat cagcacacca gaaaaagtca tcacaatcag
    15421 tattgagcac cggaatttgg agaaagtgag caaagctttg gaatacttac aactaccctc
    15481 accttcgatg aaagttctca actatgtcga ttccctcaag gtgtggatag aaggaaaaag
    15541 actattttga ctatgtaccc ggtagcatta acattcgcgc ttgctgcaaa tacacatcca
    15601 cataaaataa caagatatca tccgacacga cttccaaagc acatacccat gcgcatccaa
    15661 ttcaaacaca tatacacatt atatgataca tgtactgata atatgacgtt atccttgacc
    15721 tctaaggaat ttgtaacgaa aatggaagaa aacgtcaaga gagtagataa tagagttaat
    15781 gactatattg aagattcaaa cgaaaataat atacatgaca taagaaccgc tatcagaaga
    15841 ttagatgcct catttagatc attaccaagg aagctgcaag ggagaaaaaa gatttgcaaa
    15901 tacgtaacaa caagtaaacg actcttcaaa ataaacagcc aaattagaga ttatgatatt
    15961 atttgcgaaa aattagaaaa atactcctca gaaccaattt atactaagct tacagactca
    16021 ttgaacagaa gaagaaaaac aaaacttgct agtgcaagaa aaatagcatt gtcactaaaa
    16081 gaccttcctc taccacatat tgtcgaaaat gacatacagt caaaaaagct agagatgaga
    16141 tttaataagg tggtatgtag attatgtgaa agaatagaac tggatcttcc agtagtactt
    16201 acaaatgcca ataaaataaa agaattacat gaaatgagaa aagattgtaa aaagttgcgt
    16261 tatttattag atctagttcc acatcagaat agtaataata ttgatactaa ggaaatgcac
    16321 aaaatgatta cagagttaga agatattcaa gatatgttag ggtctataca tgatagtgac
    16381 atcacgatag catacctaaa tagagtcaga catcccaacg aagttatgca tattcttcac
    16441 gacgaaatct cggaaagaaa taaaaaatat ggggatttta tccaattttg caaaagaagt
    16501 ttatcggatt ctagacataa tttccttaac cagattgcgc ttttgacatg aatataagaa
    16561 gttaatttaa cgagaattgg cgttaaaacc aggaataaac ccgcccgacg aaattaatgt
    16621 agtagtggag attccaaaag ggagtagcat caaatatgag atagatccaa aaagtggaga
    16681 gatatctgtc gacaggatat tggttcctgc aatgttttat ccttgtaatt atggctttat
    16741 tcctcaaaca aaagaagagg ggtggtggca tagatccaat tgatgtattc atacttggaa
    16801 attattcttt attgccccaa gtcagtgatt agctgccgtc ccatcggaat attatcaaac
    16861 cgaggatcaa ggtggcatag atccaaagat tattgcagtt ccattaacaa agatagatcc
    16921 caccttttca actattctag ctatcaacga cattccagag aatgtacaaa tgcagcgaaa
    16981 gaaattgctt cctcggttaa gcagctgcaa agaactacac caacggaaat aatttgtttc
    17041 ggttaatgat ggaagggggt aaaggtagag gcaagcccat catcaccaca tgattcaact
    17101 cattcctcta ctccacccct ttatgacttt cgtttcgggt tgtcggcaat gtctatcaaa
    17161 atcgattatc gctaagaagc acaaaaaaga tagaggaaat actcttcttt actgaatctt
    17221 tagatcctaa cggtgctgat ttatgaagag gtaagagtga tgatggtaat gcaaagaagg
    17281 aatgctttca agatctaatc tgaatttata cattgtgaat tattttatat cctcgttgct
    17341 tatttaatta gtcaaacact ccaaactgct ttgtaaattc tttaaacata gagggaatgc
    17401 cccatagcgt catttaattg gccaatgaga atcaatttgt gtcagctatt agggtcgcca
    17461 ttcaacccag tgtggcgcat tggggtattt tcaatttgtc atctaggaag aaaagatttt
    17521 acactataga atagtaagaa tatatagtct gcagcattat acgatttaat atgtttcttt
    17581 tgccaatggc ttatattcaa gggattctta ccattatcat aagcctcaat agatgtaaat
    17641 actgattgtt cagaaagctt tgccatatcc attgttatag tttgaagacg atctaaacat
    17701 aggtctaaaa gtctagtcaa aaggataaca ccagaccaaa ataatacatg tgttagccaa
    17761 atttaccaga tatataacgt tctgtgagct ctttttcagg attctcaaat atcttctttg
    17821 taggattatg ctctattaac tctccaaggt acatgaatgc agtgtcatca gaaactctgg
    17881 cggcttgctg catattgtgc gttacaataa ttactgtata tctttttttt agatcatgta
    17941 ctaaatcttc gattttggaa gatgccatgg ggtcaagtgc agatgttggt tcatccatca
    18001 ataacacctc gggttgcata gctaatgccc tagctatgca aaggcgctgc tgttgaccgc
    18061 ctgataggcc cattcctggt ttacctaaat cattttttac ctcatcccaa agtgcagcac
    18121 cctcaagact ctctttaacg atgtagtcca ttaattgttt atcttttatt ccgttaagct
    18181 tcagtcccgc tgcaacgtta tcataaatac tcatggtagg aaacgggttt ggtttttgaa
    18241 ataccattcc aacacgtctc ttgataatta caggatcgat atttctatca taaatatcga
    18301 gaccatctat aataacttgg ccctttgcct ttgcgccagg ggtcatctca tgcatacggt
    18361 taagacattt gatgagagtt gttttaccac aacctgaagg cccgataact gctgtaactg
    18421 ttttctcttt gacatccaaa ttgatgttct ttaatgcttg tttagttccg aaccacgctt
    18481 ctaggttttt gatagatact ttatgaactt gttgttgttc ttgctgctct tgttttgatt
    18541 gtatctttct atctatagct ttatcaacct tgctgtcttc ttcccttagc ttttctccgc
    18601 tcgtaaacga ttcttcgtct aatcccaact tggttctcct ttgtatttta ccttaagcat
    18661 tcttatttgt ataggtggca ctcatctcgc tgcaatggtt ccaatcttaa atccccgttt
    18721 gtgcgctacg agccttagac tgaggctcaa accaaggaca agaagtataa ggacaagcgc
    18781 agccccccaa ccaaagtcat gggcatattg atatggttga gatgctaacc tccagatcct
    18841 tagaggtagt gcgtctacag gtcctgttag gctcgtaaag tacagatctg ttcctagtat
    18901 tgtcatgatt aaaggtgctg tttctccagc tatccttgat acagctaata ttattcctgt
    18961 gattacccca ctctttgcgc tcttcagaac tatatgcatg attatctttc ttttcggaat
    19021 tcctaaggat tgtcctgcct ctctcgtaga gtttggaaca attttcagtg tttcttcagt
    19081 aactcttaca acaattggga tcataattat tgacaacgca aatgctcctg cccataccga
    19141 gaatgaacca agtgctataa ctaacgtaac gtatgctact atgccaatca ctattgaagg
    19201 taaacctact agaacatcgt tgaagaacct aacactcgaa ggaaatatcc cattccctgc
    19261 gaattctgat agataaatcc cagcaagcac ccccactggc gctccaatag ctgatgccaa
    19321 accaacaaca atcaatgttc cctcaatagc aggacctatt ccgcctagtc cagatcctat
    19381 ggctcctggt gtcagtgtca agaaattgat gctcaaagct ctggcaccat taattacaac
    19441 ttctatcaaa atacttccca acagtactac tgaaagtata acagagaata ttgctgatat
    19501 gctaacgatt ttgtctacaa gtttacgccg tgaagaccgc cttaacatca tgtcttgaag
    19561 cctttctttg tagttagttt tggattcttc ttgcaaattt tcctttactc ccttaatcct
    19621 gggctgactt tcaccattct tgaaactagt agccgcgctc caatcaagat agccatagtt
    19681 aacataagca gaacggctcc caaaccaatt aaagcagata gatgaagatc agaagatgcc
    19741 tcgttaaatt cgttagcgat caaacttgat aacgtttgac ttggagagaa aagggaggtg
    19801 ggaatagcgg ctgccccaat ggcatttcca atgatcagag tcacgagcat tgtttctccg
    19861 attgctcttc ccagtcccat cattgctgcc cctagtattc ctgactttgc gtaaggaaaa
    19921 atcgctatcc taactgtttc ccatcttgtt gctcccaaac tatatgcagc ttctctctga
    19981 gagtctggga cagccttcat gacttctctg catattgaag atacaatagg aatgatcata
    20041 atcgcgagaa ctatgcctgc tgtaaaccaa tcaagaccaa atggagttac cgcaaaaaat
    20101 ggaattgact ttccgagggt gttgtgaagc ggcatttcaa ttaaattgag aatccagaat
    20161 ctgaaaacaa acaatgccca taaaccataa attatgctcg gtactgcggc caacaattct
    20221 ataacaaaag ataatggagt tctaattttc gataaaatct tcgaggaaga catctccgta
    20281 agaaatatcg caattcccaa gctgattgga actccgatag ccagggctac tgcagaacta
    20341 agcagcgttc cgatgatata tggcaaagct ccgaatgatt cacggccttc gaccgagttc
    20401 caattagtac ttgtaaaaaa cttaaaacct tcaaccgaaa atattttctc agagccgttt
    20461 cctactgcat ataatattag agcaagcatc aaaagggtat atgcagcagc acctatcgcc
    20521 acaatataga aaatcttgtc tcctcttcgt ttcccctgaa gaaaaaaaga agacaacttc
    20581 ttgttacgtt gttcacgaga ctctgtaaga atactgatac gcctctacaa ttgtaaagaa
    20641 cagctatgat tatttagaat tgcttacaag aataatatag aactatatca tctatataga
    20701 gagattaacc tgataaaagg taaataaatg aaaaaaaggg tttttggaat agcttttttg
    20761 gaagctttac ttccaatgtt atagtccgcg agtgagaaga tttcagtatt tgcaaagaat
    20821 agtaatatta agaagatatc ggcgccattg cagtccagat tctccacaac tcataagcta
    20881 gaaagagaaa ttgccaatct tatatctaat cctgtgtggg acaatctcga ggtatcaaag
    20941 atggagtaaa aattcaccac tatggccaaa tcgatgtaag acgccaagtt cgttgtagat
    21001 acttctagtg ggccaaatcg ttcttcgata taatctcgga gcttatttta gagatttgtt
    21061 ttgagtgtga ccattttctc atccactgta gtaagtcaac tattgattca acgagttcct
    21121 gccccttaat tgttagcttg tattctactc taggagggat ttcattatat gcttgcctct
    21181 ctagaatccc acccttttca agttctctca gtcgaatagc aagtgtttta ctactaaagc
    21241 cctttaatgt ctgtctaaac ttcatatgtc ttactggatt tgtagtacaa catagtggaa
    21301 tcagaatttc aagagttcct ctttttgtaa tcaagtttct tagttttgca gtctcagcca
    21361 tgagcgatgt tgcatcatag ccttcaaaac aacagccttg ttgctgttcg cgtattgttg
    21421 gtaattctac tcttcgtctt ttagtttcca taattacact tactttatct gttttccctt
    21481 gacaacttaa atagtttctc ttgtaaacaa cactatgaaa ggccagcaga ttaaagaaaa
    21541 agtaaaagaa agatatggaa agattgcttt agcaggaaat tctgaaggtt gctgtacacc
    21601 tactgaatgt tgcggagatg gtgtttcatt aaccttgcct gttcaaattg cgaaaaatat
    21661 tggttatgat gcaaaggaat tgaaatctgt ccctgaatct tccattcttg gagtaggttg
    21721 tggtgccccc ataaactttg cagatataca tgaaggtgaa atagttgttg atataggctc
    21781 aggtggagga attgatgtat ttttgtcagc aaacaaagtc aaagattctg gaaaagtatt
    21841 cggtattgac atgacggatg aaatgctaga aaaggcacga aataactcaa aacagagtgg
    21901 atttacaaac gtggaattca aaaagggaga tatagaaaga ggaattccgc ttgaggatag
    21961 tactacagac gtagtgatca gtaactgcgt tattaatttg acccttgaca aagtcgcagc
    22021 cttcaaggag atttacagaa ttttaagaag caatggcaga atggtaattt ctgatctggt
    22081 tacaagtaaa gaggttggcc tagattcagc aaattcggag gattggtgta gttgcataga
    22141 tggggcgtta acaaaggaaa actatatcga aattatccga aaagcaggat tcgacaatat
    22201 catggtattg caagaaacgc catacataga agaagacaaa gccgacagta gaaaaataac
    22261 gagcgtcgtc ataaaggcag taaaataagg gaacgcaaaa atgaaatttt ttggcaagtc
    22321 gataggctcg gatcagagta aacaggctgt gttatttgta tgtgtacaaa atgcaggaag
    22381 aagccaaatg gctgaagggt tctttaggaa atatgctcca agaaactacg aaccaataag
    22441 tgccggaacc acaccagcga acaccgtcaa tcctcttgcg atagaggtaa tgaaagaagt
    22501 taatatagat attagcaaac aaaggcccaa gataataacc gaagatatga ttaggcaatc
    22561 tactgccagg gtaaatatgg gttgtattca aagacaatcg tgtcctactc tattcatcca
    22621 cgatgtaata gactggaata tagaagatcc taaaggcaaa tcaatagaga aagtaagaga
    22681 gatcagaatt gtaattgagt ccagagtaaa agaacttgtc gcaagtctta gtcaaactag
    22741 ccgacaaatt aactcctaat caaaagaagt tcgtagctga agtcattggc acatttattg
    22801 ttgtagtgtt tgctactggc tcagtagttg tagattctaa attgaatgga gtattaggaa
    22861 ctccatttat tgcatttgct ccatttgtag gtatagctat tggagtctac atatttggaa
    22921 agatttcaat ggcacacttt aatcctgctg tcacaattgg gtttctaatt acaaagcata
    22981 tcacaaaaat tcaactggca tattacttat gtgcagaaat aattggtgct ttactaggaa
    23041 gtctattcgt aaaatacgta atcgggctta atgcgaacct aggtgcaaat gcgccaaatt
    23101 atagtttccc tataccagtc atttttggaa tagaagtatt ggcttctgct cttcttatga
    23161 cggttatttt tacggttgta tatactaaag gtttgaaagg ttttggtgga atcgctatcg
    23221 gtggaatagt tggcctagat attttctttt tggcctttat ttcaggtgca tctatgaatc
    23281 cagcacgttc attagctcct gctttactat ctggtgtatt aggtaactta tggctctatt
    23341 ggtctgctac ttttgttgga acatccatgg tagcatttct actaagaaaa aaatttgtca
    23401 atatttcaaa gggaagttga gtgtcgttac aacacctaat ctatagtcag cgaaattttg
    23461 ttggagccaa cgtagctccg ttttgctaaa tcgttgtgtg gtgttctcta aaatctggaa
    23521 caaattttaa taaatagttg tagaacacga atcagtttat ctagaattaa gaaggattca
    23581 ccaaaagcga gcgatgagaa aatgctaaat tgtcaaataa ttacgtgaag caggttggag
    23641 cctctcgtgg ttttcacctt tggatactca ttaggaaatc ctaaggaagt cattcaaaaa
    23701 ggatgagagc gcatttaaat ggcacccatc tactatgaaa tttgaacttt gaacttaaac
    23761 taccttccat ctgattctca ttcctcacat tgtgtttctt ttgaatttgt tttcgtggtt
    23821 tagttcgaag aacagatacg cctttgcacc ttacagtaac tacgtattcc aatacaagtc
    23881 atttcgtatc atctttaatt tgccactgct actgctgcta tcatagttga tacccatgtg
    23941 taaatcgctg gaattaaatg caaatttacc tcgcatgaaa taaaaaattc gtggagaagc
    24001 ctctgcagac atttcggttt ctttatggtc ctgtatatag aggagttccc ttgaaagtta
    24061 atgacttcag tgtgtcttga tcgtgcttca ctacttctgc aggaagtgga acataaccta
    24121 ggtttgccgc aagcttttgc ccatcagtaa ttgcccatga tataaagtcg actaacgctt
    24181 ttgctttggt ttgatctaaa ctgggattgg tgctcaagtc tttgggtata agcagatacg
    24241 tgaaggttgc tataggatat gtgctcgatc ctggtgagtt taacaacgaa actttggtcc
    24301 atgattgatc gcctgctgga agagatttag ctaccggcgc attggctact gccgcttcag
    24361 ttgaattcaa ggaaggtgcg ataaagttgc ctgcagggtt ctttatgaga gcataaggta
    24421 taccagtggt aagtgcataa ttcagttcta cgtaccctat ggtattgggc gagcctttga
    24481 gagcatttgc gacaccttca ttacctggtg ctcctattcc gactggccat ggaactgact
    24541 ttgaggctcc aatggtctga ttccacgatg gacttgcttt agagagatag cttgtccaga
    24601 caaaggttgt tcccgatcca tctgatctgt gaacaactac aatgttagct gcaggtagac
    24661 ttactcccgg atttaacgct tgtatttgcg gatcattcca ttttgttatt ttaccctgaa
    24721 agatgtcagc aaggactgga cctgtaaatt tcaaagtctt agttggaatg ccaggtacgt
    24781 tataggctat tgctactgaa cctattgttt ctgggatttg gacagcccct ggagctgctt
    24841 gtcgttccgt agcagtgagg ggtgcgtcgg aagcaccaaa atctactgtc tttgcagtaa
    24901 attgtttgac tcctccacca cttcctatag attgatagtt aatgtttgta tttggatgta
    24961 ccttttgata tgcaacacgc catgtatcaa ttaatggaaa tgggaaggtt gcgcccgctc
    25021 cattaagagt cacctggctc tgcgctaagg acttttgtga tgcacttaca gagattatca
    25081 ttgcagataa caatgcaact acaatcattc ctaataatcg ttgatttctc gtttgcatgt
    25141 caatgcttat tggaattgtt aataattatg ttatggcaat aaatcacata gcatgcgtac
    25201 tactatacga tcaatataga tacaatagat ttataattta tcttattagt tctaatcata
    25261 cgaagagtat gctctacaga tttgattcga tccggccaac gatttttaaa aaactgatag
    25321 ttatatatta gataacttcc gtttttgaaa taagtaagta tatttgtatg tttgtagatt
    25381 gcaggattga ataatgaata atgtcacacg gatatcttaa tgatgtttgg ctacccatat
    25441 gctacaaatc ccatgatata attccttgac gagattttga attttgacaa tagaaataga
    25501 tcgctaggat caatggaagg ccaattcctt gaatactttg cagggttaac cttctttatc
    25561 tgcacatgat ctcatttcct tctacaagtc aacaagggat cagtatccga ggttactgtt
    25621 tttatattca tttggatact ttggtaagcc tagcaatctt tcttttgata gttctccatt
    25681 agctaattgt tgtacttaca cacata
//
LOCUS       DAOR01000054            4224 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_115603, whole genome shotgun sequence.
ACCESSION   DAOR01000054 DAOR01000000
VERSION     DAOR01000054.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_115603"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1367 a          755 c          837 g         1265 t
ORIGIN      
        1 gaagaacaga agagaatgtt ggatcggata tttattttga attacgacga taattttagg
       61 ctaaagttag cgcaagaata tatatatata ttatcagact tttgtcttgc acgaggtatt
      121 gaaagacaaa tatagaaatg actattttga caaattatcg aaatggattc tattcccatc
      181 tttctaaagt acgccaggta tcgatatatc gcatacagat tatttttacg tataaggatg
      241 ggcaagaaaa agcgggatga gttcctaaga ggaagtgggt tatctgaaat agatttcttg
      301 cctgaaaggc cctattctat taatgggatc aatgcccttc caagaaaggg gacagatgac
      361 ttttatatgt tctatatacc acgggaaaag gagcttttgc ttcacctgat aatgcataaa
      421 ggcgaaactt ttgtggacgt gggggccaat gttggctatt actcgttaaa gatcgcgaag
      481 gaatactcat caaagggggt aactatcata gcaatagagg cgcaccccgg aaattacaaa
      541 gcactaagta aaaacataga gttaaacgat tttaaatgta ttactgcaat taacaaagca
      601 gtatcagatc acaaggggat agtgacactg tatgacagag tagacacgag aaatcgtatt
      661 cgctcggaaa tgtatagcct ttctaatgca tttctccacg aatcaaatgt cgctcggcct
      721 gaaggagggt ccctagaaat agaatgtgat actctcgata atattttggg agagcaaagg
      781 gtcgacgtca tgaaaataga catagaggga gcagaggtat ctgcattaga aggagcaact
      841 catattttga agaaattgcg caaaattatc gtggaagtgc atggagccaa tttcgataaa
      901 gtaatgcaaa tacttgatga tgcacataat ttcagatgcg aaactattga aacgcgaata
      961 atgaaatata tcgtaggatc taagttttcg aactagtctg cggatagaca ttatattgat
     1021 ttgatctgac gtgttcatat cgtgcttatg cacgtaccgg atgatctaac tcaatgacct
     1081 ctctctccta tcgcaaacaa agaagagtag aaataattct ttatctatct tttttcggta
     1141 ttcgtcttgc ggactatacc acttatctgc ccgatcttgt tttcaggaaa gatcaatctg
     1201 tggacatgca attttcgggc tagttatcag agatagaaat caatcgttcc aggatcagct
     1261 gcgggttctg caagcaatct cgtaaaaact tgtgcgaatt attacatctg tactaaattt
     1321 gaaacataca tgttaaagaa ttttgctcgc gtactcgtgc ttttgaaaat gaagaaacaa
     1381 ggtttggatc gctatcactt gtcctggctt acttatttag agctcaatta gtcctcattg
     1441 gaagtagaaa ttacgaaagt gtaaatcagt ttatattccg cgacccggct ataacagata
     1501 ccttttccga tttttatttg ctatttaagc actgcgcacg gaaaacgaag tcaccaggca
     1561 aagcaaactc gtataatccg tctggcatat cttgctgacg caaaggctat aagttttttt
     1621 attctgcctt gtaagttttc ttattaaatc tcaaaattat attttttaac ctctgaactt
     1681 ggttctctct gatgtactgt cgaatattct tatcatacat gctgtcatag ccaactgatt
     1741 taggagtata agttaggtaa taataaagca tataaattgc cccctgtcta cctgttactg
     1801 ctccagttac aaagtatttc aaaaatctgg ccgcagcgaa tagaggaagg taccccaatg
     1861 tacgcatact tgcaccaaat tcataaaact tgtgtgtttc cccaagaggt cttatatgct
     1921 caaatggggc atcgcctatt acaatataag tataaccacg atggctggct tcgtacagaa
     1981 tggccgattc gtagcccatt tgctctggat aataaccatg ccaaatggtc ttttcaaaaa
     2041 acgaatttct gataaatctt cctgctccgt gtggcataac agatctatat ttagaatagt
     2101 tccctgaagc gatggcgact gatggatttg aatccatatg tgaaattatt tttttcgcat
     2161 aatcgggcgg ataaattgta tcgtcggttg ctatcatatg gtaatcagtt ttttgcaaac
     2221 cgctgtttcg attcagctta atagcagcat tccagttctt gacaacccta ctaatatcat
     2281 atcctaaatc agggttagag atcacatgga gagaaggcca atctttctgc atatcctcta
     2341 gtatatcttt tgttttgtct gtagatccat cgtttatgac tataacctgt tcagcattga
     2401 tcgtctgctc cttgatcgaa aatagggccc tccttatagt ttcttgtgaa tttcgacatg
     2461 ttactacggc aaaataggtt tccacttagt tgaattttaa aacagatcaa agttattaat
     2521 attgttctga ttggttaaca gaagaaacaa aggataggat agtcctacat gtggtctaag
     2581 gctacatatt ccaaactaaa gtcgtaaatt agaagagtat acctcttcgc attataacaa
     2641 agcttcccaa tatctcaggc attgcctccc attattgcag agcagcaaaa aagattattt
     2701 atcaaatttt ttgcagtcta atttactcgg gatatggtta ctcattacac atgtgtttca
     2761 tgcagataaa acctcgagat ttgagattgc atcatatgtg aaaacttaga tacgtgatgg
     2821 ccgataataa ttggctccca gaaaaaaagt ctcaaatttt gagaagcttc ctcaagctat
     2881 gttgataaaa gtttcgatag ctataaacgt cttctcttat ttccaaatga atggatgaat
     2941 gaatgaatga attgaattgc caaaaagcct atgaaattga atgtttaaat tagaagagaa
     3001 tacttcttcc cactataata attataaagc ttcaacaatg aaaatagcac atataaacaa
     3061 tacctctggc attgcttcag ttattgcaga gcaacaacga agagaaggac acgaggtaga
     3121 tgtctttgta tttaatgaca cggtattcaa gcaatttggt gggagtaaat tgaactactg
     3181 gtcacctctg gatagatgga aactctttag aagattaaga gggtatgatg tatggcacta
     3241 tcactatccg tatggctccc ttaagagaag tctggaaaag cgaaagagtg ataaaagata
     3301 tctaaaacac tatcacggca acgatctaag ggatcgacat gagggggatt tttgtctagt
     3361 ctctacgccg gatcttttga aatatgcccc aaatggcaga tggcttccta gtccaataga
     3421 tctactggaa attggggcca cgagcgaaga actagtttct gctaacaaca aagaaaagca
     3481 agaaagcaca attatcaaag ttgctcatta tccttactac aaaaattatt catcattcga
     3541 ttactacagc gacgtgcttt ttagattaca agaagaaaag aaatgtgagg taaccagtgt
     3601 tttgcatcaa ccacatttgc aggctctaag gattatggct agctcggata ttataattgg
     3661 taaaatatta ccagacgttg gatggtttgg aaaatttgaa cttgaaggaa tggctcttgg
     3721 taaacctgtc atcgcgtatg tttcagagga gttgtatgaa aaatataagc ctccgattta
     3781 cagaaccaca aaagatacat tcagaaagga cttggaaaca cttattgagg atcgtcttga
     3841 aaggaaacgg ctagctagcg aaggtccgat atacgtacga gctaatcact ctgtagaagc
     3901 tgttgttaaa acagtgatga tatgctacca ttgacaatct cgtgcttaaa tttctcaatc
     3961 gactctagga agtagtagta ctaattctgc gaacacgatt agataattat aattagcata
     4021 gcgacaaatg atattttcgc acgcttgaaa attctacatc tctcacatga atccttgcct
     4081 gattggagga tcgaaaaggc ggcgatatct gctttgaaca atagccatga ggtagccttt
     4141 gcaggtatat acccaaaaga ttacaataac gatacttttt ctgaaatata tagcatggtt
     4201 tggaccgcta aagccaggtt cggc
//
LOCUS       DAOR01000055           11345 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42416, whole genome shotgun sequence.
ACCESSION   DAOR01000055 DAOR01000000
VERSION     DAOR01000055.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42416"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3213 a         2423 c         2223 g         3486 t
ORIGIN      
        1 atgtgtggaa gcagcacaat tagttggcga tccaggtcga cgcattagaa aatattctta
       61 cacgcctagc ctaatacaga attgagataa aagattaaca aagctaatct atctttccaa
      121 tcagatgatc ttttaaccac acatacaacg gaattatagg atgttgaaat ggcaaggact
      181 tctcattttt tctgttgaag cctagcatta tcatcctacc ttcaagataa tttcttaaat
      241 ccttcaatag ctcatgtcct ttaagtggtc cataaatctg gaaaaaatat tcctgattat
      301 agtaagagac gtgctgcaca aagcaatatg ataacacagt gggatcgtta actattcgct
      361 ccttatgaac cgctttcttc tcttctgtcc tgatttgtaa ctctctttca atgtatccac
      421 tgcgtataaa ttctgtgttc attcttgttt ttggaaggtt cttgtaacct tcgatttcga
      481 gatagaattt attgacagtt tcagatgcca agtccattac ttcccatttt tcattattgt
      541 caactttact aggtcttccg aataggaaca ggacacaaat tagctcataa ccttcaacaa
      601 acggaaaatc ttgtttccct attaagaatt cgcctatgcg ctcggaaata ttttccggaa
      661 gaatgatgcg catatgtgaa ctctgtcgct gcactcattt attatatgga ttgataatat
      721 ataagatgtg tatgctttat agttactcag aagtggatta ttcaacaatg tcagatgcag
      781 gcgttaaaac cgtctatcta atagttcatg ggcaaaagca actactcgag caggtaatag
      841 aaaaactagt tgcacgaggg ctccaacgga gtaatatgca accagcgagt cttgaaaagt
      901 caggaaataa aggagatctt gtcgcgatgg tgtggcctcc tagtacgccc aaggagatcg
      961 ttgttagtga gatcactggg aatcgcccga gtgaaagtca agacataggg attgaccttg
     1021 gcgcctggac ttccgtaggt caaaaggagt tatatcgaat atctctggga tgataccttt
     1081 ttccctatgg aagagtgttc ttatctagaa aaaaagccga gctcaatggg catgtggcgg
     1141 cggtgcacta ccttttgaga gagcttcatt gaatgtacct gatgcttttt cgtggaattc
     1201 aagattagac tgatctactt taattgcctg aggcggacaa actgagacgc aggccataca
     1261 ccaaatacaa tcgtgttccc tgacaggatc ggccttgtca gtgtagtctt ttctctcctc
     1321 ttttacggtg ctaccagttc cagcgctcgt ttcgttcacc atcttcgaag gtggtacatc
     1381 ctgctcagtt ctgtaccact gaaagacttg gacaggacag gcctcaatac acgcaccgtc
     1441 tgctacacag gagtcccagt caacggcgac catagtacca tgtattcctg ccggagcgat
     1501 ttcctctcct ctagcagtaa atgcagcttt tacctcctca ttttcagcag cctctgctgt
     1561 tttacctggt ccccacacat agtgataatg ctcttcgtca gaatgcacat gtttaccaat
     1621 tacttgatta ttttttggaa agtctggatc tattggcata tctatctgtc agatagtcga
     1681 gattacatat tatataaacc atatgtgaac ggaagacatg agtatctcta ctcgtgttgt
     1741 tatcttaagt aagtgattgt tctgattaat ggatgcgtta cccaatcttt tcaaataaga
     1801 cagtcaattc gtcgccctga agtttcgctt tggcaagttt catacccata gtggcagcag
     1861 gaaggggcac tgtattcacc ataaagccga ctcttgtttt cacctttatt gtaagcttat
     1921 cactatgacg ttcaacatca aagtcatctt tctctgtgaa cggaactttg actgtcattc
     1981 gtaataaaga attctcttta gcaaagtgaa atgcgtttcc acgaaagagc acatcagccg
     2041 gatcttgatt ttcgaacatc agttgaccat tttctcgtaa catttcgatc ccccgtaatt
     2101 cagtgccata gagtttaacc tcctttacag ggagtgggta aaagtttgct ttggcttctt
     2161 ctatctttac tctttgaaaa tctgcccatt cagcatagta tagatctgag ctccccgtgg
     2221 gcataatctt attgattatt gcaagatcta catttatacc ataaagactg gcagacatga
     2281 atgctctctt agcattctct atgctaaatg tgtcaggatt ggtaactagc cttatactcg
     2341 ttatgtcctg gttcatcatg atcatattta ggttgtctag tctggcaagt aactcgagtt
     2401 cattttgcag aacattttgt ggaggcgtag gtagccgcga cagaggctgg aatattcggg
     2461 caaagccact gaacatcccc accaatccgg taagtttcct tccaatactg ccgactaatt
     2521 taggaaaata gagatatcgt aatgcctcgc ctgaagcagc catgtcaaga accaccgtgt
     2581 caaatctttt cgtgcgagct acttcttcaa ttttcaataa tgaaaataat tgagtcattc
     2641 ctggcaacat ggcaagctcg taagctaaag tttcatcaat accccgtgca tgaaacaaag
     2701 ttgccatgta cgagagaatc gttgcatatt gtttgttcat ctccattagc gggtcgatct
     2761 gcagtgcttg caaattcgta agaatttctt ttggttcata gcccaggtcg ggcatcgcga
     2821 aagcgtcggt taaggtgtga gcaggatcgg atgaaatcag taatgtacgg tatttattct
     2881 ccgcaagttt tatggctgtc gagcatgcac ttacggtttt tccggtgccc cccttgcctg
     2941 tatagactat taaccgcaat attcgttctc gcgcatctta agtaataaaa agtatacagg
     3001 gttcaattta aatcgccttc tcaatgctag ttgcaatacg ctccaaatct tctgcaccca
     3061 taaaagaatg cacaggtaat gagagaacgt gttcgctcgc ccaatcggtg ttaggaagac
     3121 tttttgttga tgtaatcgcc tgatagtaag gggttttgtg caccggtgga ttataatata
     3181 ccgttgcacc aattttatca cttgccagtg cccgtcgaac cttctctcta aggtagtctt
     3241 tttgaaaagc aaccgtgtac agataccagt taaatctttt tttcgcagtt tcgtgcggga
     3301 tatttattct cctttccttt gtcaaaggaa ggagtaaacc tgtcaacaca tccgcgttcc
     3361 tcctccgaag atccagcaat ttttgtaatt tatccatttg cgctttggca agagcagcac
     3421 tcaattctgg gagcctcagg ttaaggccta aaacacgggt gtcataacct tcgaccatcc
     3481 catgattcct aatcatcttt atcttatttg ccaagttatc atcattagtt gctactgcac
     3541 ccccttcgcc agcagtcaat actttgctcg cataaaagct aaaacagccc agttttccga
     3601 gtgttcctgt ttgcgcattt ctatatagag atccaagcga ttgacaagca tcttcgatta
     3661 tatctagtga gtgtgcatac gcaatctccg aaatttcatc catatcagcc ggatgaccat
     3721 agagatgcac tggtatgata gcctttgatt tttttgtaat ttttgtcttt aggtcagcga
     3781 tatcaatcgt atattcttct ctgttgatgt ctacgaacac aggcttagcg ccggccgcaa
     3841 caacagaatt tgcggttgca acaaatgtga atgacggcag tagtacttca tcaccttgac
     3901 caattcccaa ggcgagcaat gcagaataca acgcagcggt tccagaattg acacttacta
     3961 cgttttggac tttaagataa tctttcagaa ggcgttctag agcctgaact cgtttcccgc
     4021 catcttttgc tgcggatgta agagcacttt catctaaaac actcaatact tcacgctttt
     4081 cttcctcacc aatccaaggc tggttaattg gtatcattga catgtgtttg aaaagtactc
     4141 ggataaaggt atactgctta tcaataaacc tgctacactt gtgtctacaa tatttgtatt
     4201 actcaggcct gtgagcttgt gaattctgag tgatgctgag ttaggatgga gaaatactgt
     4261 ggtattttct ggttctatag acaagccgag tacgcaaatg cctctctcgc cagccataca
     4321 agctgcctac taccttgttg caatcgatgg atgagttaat agatgatgac tttgacaaga
     4381 gatttggatg tcattcctaa aataccggct tctgcgaatt cgtaagttta caggaaacaa
     4441 gatctatctt gtaattcgat atcttttatt tttatacctt aaattgaagg ttagattagg
     4501 atggtattaa gagaaatcgg ctcatttctt agctaattga cttagtagta taccagcata
     4561 catattacgc aaagaccttt ttagaccaaa tcagccatct attggggctc tatcgtagct
     4621 ggtggtttgc ttgaaactgc atacgttacc caggagacat cttcgacttc gtttgtaatt
     4681 ctatcactca tcttaccaat tatttcgtac ggcagcctag tccaatccgc agtcattgcg
     4741 tcgatggatt cgacaatttt aattgtcgcg atatgaccat aaatacgctg atcacccaaa
     4801 acccctaccg ccctgtcatc tcccactgaa gcatatgcct gccatacctt atgatagaat
     4861 cccgcagcat ttaattcatc ctcaactatt ttacttgcat gtctggcaat tcggatcttt
     4921 tcaggtgtaa cttctccgat tattcggaca gctaagcctg gaccggggaa cggatgtctt
     4981 tgcagaaatt catttggaac acccagcaat ttcgcaacac gtttaacctc atccttgtac
     5041 aaagtcctta atggttctat tgtctttaaa ttaagccatt tcgggatccc cccaacgtta
     5101 tggtgagttt tgatagccga cgcaggccct tttgatactc cgctctcaat cacgtcagga
     5161 tagagcgttc cctgagcaag ccattggaaa ggtccgtttt tttttaccac atccataaac
     5221 acctttgcaa actcttcacc tataatgctg cgtttcatct caggatcgca aactcctttc
     5281 aacttttgaa ggaactgttt ttctgcattc acgtaaatca cttccgattg caggtaatct
     5341 ttgaaaagcc cggttaccaa gacctcttca tcttctctta gtaacccatg attaacaaaa
     5401 acacacttca gatttttgcc tatagctttg tcaaatagtg ctgcaacagt cgtggaatcg
     5461 atacctccac taacggcaca aagtactttt tcatttccaa cccgttgtcg agtgtcctca
     5521 atggttgatt ctataaaact ttccatgttc cacgaaggtt ttgctcggct gatcttttga
     5581 gagaaatttt tcagtatctc acttccgttc tctgtgtgaa ctacctccgg atgaaattgg
     5641 attccataga gtctcttttc ttgatttgct atagcagctg agaatgagtt ctccgtatga
     5701 cctaaaattt cgaatccctc tggaaggttc tcagcagcgt ctccatgact catccaacac
     5761 tttatcttac tgtcaattgt gctgaacaga tcgcccttat tgtcaataat caggtcagca
     5821 cgaccatatt ctctggtact tgtttttttt acttttccgc caaaatggtc tattatcagc
     5881 tggtgtccat aacagattcc caagattgga atccctaact cgaaaatttc tttatcaggt
     5941 tttggcgagt cattgaaata aacgcttgcc ggtccgccag agaaaataat gccattcggg
     6001 cggatttgtt ttagttcttt aatggatgtg ttataaggta atagttcaca atatacgttt
     6061 atgtctcgaa tccgtctaca aatcaggttg ctatattggg aaccaaaatc caaaacgact
     6121 atattgtcca ttttttactt tccgccatcc ttttccatca tccttgttag aacccaggtg
     6181 gacgtgttga aaatctcgtt aaggcgctct ttttcacggt aattgctgat cataatcttt
     6241 tttatcagag taccatcctt ttcttcaatc tgataattca acgattcaga ttcttggatc
     6301 tcgccccggt ttaccttttc aatatctctc gctttcattc cttccaatat tctattcaga
     6361 aagaatgtct taaatggctg ggtattgacg tcgagattga ttccatcaac aggcgtgatt
     6421 tccactctag tcggaaacaa tgtggcagtg gcgagcaaaa ggttatcttt aatacgtcgg
     6481 agttgtctaa cctccgtgaa acttgttaat gtgttatcct gttgttcagc tgttggaaca
     6541 acgactcttc gtatggcagt tatatcatcg ttatcattac ccgcggcctc ccgagtttgt
     6601 tggtaacccg caatagttga agattcatca gagggcttta ttgtcgatga agaggtaact
     6661 gactcaagac ttgcagcagt tttgaagctg cctcgtttca acaacatatc gacaaggaac
     6721 agcatggtgc gtagcctttc gatttcctct tgtttatcag cgacctgttt agtaagccat
     6781 tcacggatat cggcggcacg tctgacgacc tcttcagagt agctggacat tgcaagcatt
     6841 ttgccacaga agctataata gtttttcaca tcgagagcag atagagcaga gaaaatctac
     6901 tttcctaact tgggttttat gcttctcttg tttagttcta gcaacttaat tctgcccggc
     6961 ctgataatac aattgctaca aatgtctata gaaaacagtc gagtgattcc tcttagaacc
     7021 gctaggacca cgtccaatag cttttttcac atcctaccac tttgacgact aatcatactg
     7081 aatagtcaag gaggtaaatc ctctgatggg cctttgagat gtgtcgaaca atcgtgaatg
     7141 agaccttttg gcaagaattt ctaaaagtcg tttaccaact ttcatcttct ccttttttaa
     7201 ttcaatattc tttaccatct ttttcgagtg ctcgccaatc cggtcaccaa actccattcc
     7261 tactaggaca atttctttgg ctccaaattc atctgctaaa aacacgcatc gatcgccatc
     7321 cgtaaatccg ccaaagttat agacgttctc aacaggcatt acttgagtag atccgatcac
     7381 gcgcctgaac gtcggtacaa gtttctttaa ctcttcaaca ttatctccat ggctatgaac
     7441 caccatgatt gcaccggctc gctctgcctt tcgcaaagat aatgagtcgc cgtccaagtc
     7501 ggtaacaaca atatctggcg tgatatggtt ttcaatcagg gcttgggttg caccatctgc
     7561 tactatcttg acgaattttc gattttgctt tataaatttg atatttgaga ttaaactatc
     7621 tccagcacct atcgccatga catgtctttg agctattttc ctttgtaaca ccttaatgtc
     7681 gatcgttttc ttctttatca ttttagatag tatgttggca gcttcttgat ccttttctgt
     7741 actatatcca aattgctgtc taatatcctg gtaatacgga aaccaatcta tgaacctcac
     7801 acctagattc catccaagtt gttaataggt tgtttgttag ataaattatc attcataaag
     7861 ttcgactcac attctcgaat aaataagcaa cagaatttat attccaactt ctagctatgg
     7921 actgaggatt gcaaacgtgt ctaatgacaa atgctgcatg tttattaaac cacacctatt
     7981 ctgactacct actcttatat agctaagaga ttaataattt ctgccaccag ccactttcgc
     8041 ttcaataaga ccgaaagtta ctggtgaact cgcccacaga ataaataaat tattataatg
     8101 taacagttat aacgaaaagg gtcggttaat actctatatg gaaagccgga aacttacaag
     8161 aatgccaatc ctgcccgggg atcagatcgc atccatcgag gaattcgagg ccgggaaata
     8221 tgcctatgtt tcagatggga ctataagatc gacttcagtt ggtactaaaa tttatgattt
     8281 caaaaagcgc atagcgaaga tcgagaaaat taattctcct atattaccga gaatcggcga
     8341 catcatagta ggctacattg aaatgctgtt tggcagtatg atgtctgtaa gaatacttta
     8401 cataaatgag aagaagtcat tctccggttt ttcggccata gcatcaacta ggataagcac
     8461 acctggccgt gaacgagatc ggagaagtcg aataatattc cgggtgggcg atattataag
     8521 gggacgggta attagtttat tgaattcatc catccacata acgatcgacg aaaaggaatt
     8581 tggagtcatc tacaccctat gcttcaattg tggtgggggt actgtacgag taaacaacaa
     8641 gaccttaaaa tgtatcgaat gtggaataaa tgaagaccga aaattgacaa ttgattacgg
     8701 agaggaagcg ttcagattga ttcatcgaag tgggagatga ttttgttgcc ggaggtgaac
     8761 gggttgcgtt tcaaggcgaa cctggggctt acagcgaaat ggcaacgatt cagtattttc
     8821 gaaactcaaa tcttgtacca ttaaagtccc tccaggatgt atttgatgga ttgagtcacg
     8881 gtgacgtaga tttcggcgta gtcccgattg aaaactcaat agaaggtagt gtaaatgaaa
     8941 cctatgatct gctattagat acgcattttg tagtatccgg tgaaatctac ctgagggtta
     9001 gacattgcct tatcgttaat aagggtgtag gtttgaatat taggtctgta tattcacatc
     9061 cacaagctct tgcccagtgc aggcgttatt tacaagaaag gaaaatagcg gccgtagcag
     9121 cttacgatac ggccggtgcc gtaaaaatga tcaaacaaaa acagaatatg ggagtagctg
     9181 cgattgccag cagcagagct gcagagttat atgatatgga tattttagaa aagggaatcg
     9241 aggataataa aaacaatttt acaagatttt tagtaatatc aaaaacacga acaaagtcta
     9301 ctggtcatga tggcacatcc ataatatttt cagtcaagca tatcccaggt gccctttaca
     9361 gtattttgga ggaattcgca aatcgaaaga ttaaccttac caagatagaa tcgcgcccca
     9421 aaaaggaaaa accttgggaa tataactttt atgtggactt tgaaggccat attaatgata
     9481 aaatcgttaa ggaggctgtg gaatcaatta gaaagaaggc tgcatttatc aaaatcttgg
     9541 gctcttatga aagagcagag tttctttgac aatggcggtt tcttatttaa gcccaaaccc
     9601 ggctacaatc atgcctatga ctaccagtat tagttggcgg ctaacgcaat tatatttccc
     9661 ttagtctctg cgtgccctat cgtctacaca gaggcaccga tattcttaat ttcgattgta
     9721 tactgccatt ctttttgagc atgaaagtca taccgaaaac ttgagatggc gctttcgcga
     9781 ttcgatgagt tgtttgttgg gatctgtgat aactatgttg aagatagatc cattcagagt
     9841 aacgcttgct gtgctccctc tattcatatt cggcgccaat atatcggtta gtccatgtct
     9901 tagtgttcca ttaagtggta catcaacagg agttagcgtt taaacacgag aagcatgcaa
     9961 tatattgatt tcgtgctaag atcatgcata gaataactta ggtaacaata tgatgaatag
    10021 atccagccag catgatggcc attgcctgta ttaatagtga tcttgagcct gctactttgt
    10081 gaatcatgta ttggagagtt ttaactatca taagatttaa aggatggaaa aaattacttc
    10141 ttagttatgc caaagaaacg agctagcagg ggcaggacaa agggcggaaa gggtagctct
    10201 gggactgtcc attgcagtca atgtggtgcc atggttcctc gcgacaaagc gaaaaaggta
    10261 acgggtcgga ttactttggt tgaacctacc cttgcaaagg agcttaagtc acaaggggca
    10321 tacatagcac cgtcgacaga tgtaaaattc tattgcgtct cttgtgcagt ccataggggc
    10381 atagttaagg tgaggtcaga gtcagaccga cgtacgactg gcagactgcg ataacttttt
    10441 tgatgttgca acaattctgt gaataactgt acaagctgca actattgaga cgagcattac
    10501 tgcccactgc attgcattgg caattggtaa caagctcact attgcaagta ttaacaatcg
    10561 ttctgctctt tctccaacac ccaaccctgc caaatcgacg ccgagtgatt ccgctttcgc
    10621 tctaacgtag ctcacaagta atgacaaaga caatgctacc atgcaccata tcggttcagc
    10681 aaggtttcct acgactattc ctatgaatat cacagattca gatattctat cgcctgtcga
    10741 atcaagaaac gatcctttcg gagacaactg ttttgtcttc ctagcgacac tcccgtctac
    10801 aatgtcaaaa aaacctccga tcaggagcag aaggctgcct ataaccggta aataaaacca
    10861 tgaaaagcct gcagtgaatg ccctcgtcga atatacgatc ccagctaatg ttaaagcgcc
    10921 gagacttaaa caagtccaac cattagcaga tactccagta gaagcgaaaa ccgtaccgac
    10981 tttttccaga gttggattca ataagtccct aattttattg agcaaataat aaatacagat
    11041 agagctgttg gaataataaa ttttgtagac agtacttgct agatacattc gcaaaaacaa
    11101 tgcaagtgaa tgatttttag caaaaatcat cagattttga aatggcatca ctatttatta
    11161 tattatagtc acgatttcat cgtctatagt cgccaaacat aaattttaat agtaaattat
    11221 ttatcgtctt ttgtttctta attctttaat aatcatactt ctcggatatt acgagttagt
    11281 tgcagaaaat taatgaggtg aaagcataca caccatctag gttcgaaaag gtactagttg
    11341 ttgga
//
LOCUS       DAOR01000056            7480 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_72307, whole genome shotgun sequence.
ACCESSION   DAOR01000056 DAOR01000000
VERSION     DAOR01000056.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_72307"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    6322..6488
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2092 a         1444 c         1398 g         2379 t
ORIGIN      
        1 tctattggcc aaccccgcag gctatactga cgcatcaact aaatggaatt cgttctctag
       61 gatcgatgtt gtcaaacata caggtgatat tcgcaagacc ggtttcagag acctagcctc
      121 aatacttatt gatgccgatg ccctgactcc gatattgaaa tggaatggtt cttcttccga
      181 tcgacagtgg ttgaaggggt acatggatta tatgccgtac gaaatgtcaa aaatgaacaa
      241 tagtagtact ttagttattg gcagcggcgg cggcgaggat attcaggtcg ccttgtcggc
      301 gggttcaaag aatgtgacag ctgtggaatt gaatccgttg atagtgtctg ctgtaaaaca
      361 atatggtggg agttcagcag gaaatttgta caaccgtaaa gatgtacaat tatctattga
      421 cgatggcaga cgatttatca gctctacaga tacgaaatac gatacgatag taataaaact
      481 tgtagactct tgggctgctc agctagctgg cggttatgca ctttcagaaa actatcttta
      541 cacagtcgag gcattcaagc agtatttgaa tcacttaaat ggagttaacg gaaagcttgt
      601 catggtacgt tggagtactg aattgccaag gctggttcct cttgccgttg aggcgttgcg
      661 ggagtatgaa aaagggacaa gcaatcaaca agatattagt aagcagattt taatagtgga
      721 agatagccct ggattaacgt ttggaccgaa ccctcagcga acattctatc cagtgctctt
      781 agtgataaaa aatagcccgt ttactgcctc agaacttgct ttgataaaag agagagttaa
      841 caagagtgat gctaaggtca ttgcgatgcc aggagggtat gtacaacctc cctatgacag
      901 attgctatta agtggcggaa ataatactaa taacaataat aacaataata atcagcaaca
      961 acaacttcaa caaatactgc aagtaagtta cgatcctcaa agactgggct ttgagttaaa
     1021 accccccacc gatgattctc cattcttttt tgcgacacag ctagttccga gtcaaatggt
     1081 attgctaata gaaacagtac tcggagtatc tgcagtactt ggcttattgt tggtttatta
     1141 ttcaaaggta aacaagatta gactaacctc atcatcgcga ttccatatcg tctttgcgat
     1201 gtttattgga cttggtttta tttttttgga aataaccttc attcaaagat ttttactctt
     1261 actcggaacc ccaatcatgg ctttgactgt tattcttttt tcgatcttac tttcgactgg
     1321 aataggtgca tatttgagtg gaaagatatt tcataaaaat ccttacaagg cgataatgat
     1381 atctatacca ccacttgctg gaatagtgct actctactat tttttcctgc aagggatgat
     1441 agaatctgca atagtttggc agttgccgga gaggattgct ctggcttttg cactcttgtc
     1501 gcctactggt tttcttatgg gatttcaatt tccatctatt actagaatgg cctcttctgc
     1561 ttccagccgc ttaaaacaag agttgaatgg cagcgaagtg ggtggtggtg gttctagttc
     1621 tggctctggt ggtgaagata tcaccctcct ctggggcgta aacgtaatag catcagtggt
     1681 aggaacagtc ctgaccgcca catcatctat ggtaataggg ttcaacggta acttgctcat
     1741 tggagttgga ttatatttgg gagctttagg gtctgcagtt gcggcacaaa aaatatcaca
     1801 aaaagtaggt cataaagcga ttgttaaata ggttatgaga agttaatcgt gaaagagggc
     1861 gtccgattat tagcaaacaa gagattttag cttgcgaggc tatgcatcgt cttgacaatt
     1921 agcctaaagt gctattgcaa cccgtgcata ccaataccaa atgattccaa gtgaaacata
     1981 atcaagtggt caatgtcaag actaaaaagt tagaactatt ttctagctat atactagtac
     2041 agaatgcagc actgcgtaac actgatttgt aaccatctag ttcgaaaaac attaaatttt
     2101 aaaagcaatg agtctctaac tatcagttaa taatgggaat tacttgggtg tgtgcaacat
     2161 gttcacagcc ttttactagg cgcaccagcg caacaagaca tgacaatact aaacacaacg
     2221 gtcaagggga aattgtttct ctgcttgagt atatagtagg aagaggaacc ggccgatata
     2281 tgcccggcaa tcctttatcg tatggacgtg atcaaaaatg gcagactcgc ttggctcaca
     2341 attctaatag tggatttgga cttgcaacag ttgcggactc tataggagat agattcatgc
     2401 ctggaaacgg cccgcaagaa ccaccccggg gcaatatgta caaaccctca attcattcca
     2461 ctacttccat gttgggatct gcgtatacgg ataatctact tagagctcaa gaaatggggc
     2521 taaaaataga agaactcaaa agactcgtca tcaaatatca ggaatacctc gagaatcctg
     2581 ttggacttct aaaatgggct attcagtgct ccaataaagg tgacaccaag tttcttgacg
     2641 aaaggttggc gcagcttcac accatggaga catcttgcag ttttcggtag aagatacaaa
     2701 atagcaatat gtcaactcaa ctccgatatt atctaggact tcgctgtcaa atctagaaga
     2761 ttgggtgtgt tctgtcacta gaatttgttc atgcatctat ccatatatgg gcgtttgaag
     2821 tgaacgaatc gtcagtgcaa aaattgttag cttccttcta gcgcgaatag atagtttcga
     2881 gacgagaagt gacattcatt ggatcatact caacaggcaa accatgccta cgattcgact
     2941 tataatctaa aacatcaaga ccacaagcta atgcctccaa ggcagttttg ctcaagtttt
     3001 ctagaatttt gccatcgaca tatcttacgt caacgtacag tccatatttt ttcaaaaagg
     3061 ctggcatgtc cccgtacatg atcgggtctc tgatcctatt ataaacgtca atgtctagct
     3121 tgagattatt tttcttacaa tagctgagcg ttaattgtat atcgattgcc tcagtgtcca
     3181 tggtcaacgc ctgtgctctc tcaggtcttg aagagatctt atcgggctta aaatgatccg
     3241 tgtcaatcgg attcggaaga tatatagctt tactaactag aggtaacagg tcgggagtag
     3301 aaacgataac cgcgtctgcc agacgttgag ctttagtatg aatccttttt tttatcagta
     3361 tcaagtctct caaccttctg tatgtaaata tacctcgaac agccaagtct gatagtttag
     3421 atctgtgcgg cagcttctgc ttctttattc cgcgaatatc cgttccatga tattgaagga
     3481 tgatcttctt tgaattcccg aattcctctc gaaacttaaa aaccatgtcg ctcctactat
     3541 gtatgtgtag aatatctgca gagtgcgctt cgtcaaagct tttttgatca aaatcagcta
     3601 atgtcgcatc aatgacgtac tggttgtaaa atttaccaat tccgtattta tcatattccc
     3661 taaccatgat taccttcgaa tcgtgtccct ttaatctttg atatttcgcc aggacaaacg
     3721 cgacaccagc ctgatcccat atgtgcagaa ttcgcatggt ctattgtaga attgaacatg
     3781 agttgtatag agttactctg tttttggtat atttatcttt gaatcattgt cgagctgacc
     3841 ttctatgttc tttactccac catagggcct attagcctgt ctcattattt ttaagaggta
     3901 ttttcgcatg tgctgctttt cttcctcggt cacctttcca tcaatcagat caatcaaaaa
     3961 gagagtcatt gttgcaccta tttggttttg gttaaacaaa gcctccgctt caacactgaa
     4021 ttgatttttc ctatgccagt atccaattat catgccgacg ggaagatata ttgcaataaa
     4081 aattaaggcg aacatccaaa tattgatatt gagtgcgtga atagcaggca ccctctgaag
     4141 tagcaattgg tattgaattg ttacgaagtt agcaaatgtc atgagaaaaa ttaaatagat
     4201 gctatgacca tttctaaagt caagccaccg tcttctcatc caattatggc ttcgcatatt
     4261 gagaaatttt ggcacggatg gattcttcat tcaaatatcg tactatgatg tcatcatcgt
     4321 atatataact caattacatc ttacttaatc gtgtacaaaa ttgggcactt tattttgtgc
     4381 ttataataca aatatcctgt tttgagtaaa tgaactaact tggtttgtgt tagatctatt
     4441 agacgtacct ggcataatta ataacttaga gcaagttgtt gcgtttgctt tttctaaatc
     4501 cctttcatag taaaatgtga gataatttat ttaaaattta tttatctatc aatcccattt
     4561 ttttagagat gtcaaataaa taaatggcaa agactcacgg aggccacact agaaaaataa
     4621 tttgaccagc aagatcccac tttttgtttg caataagtga tgaagatggg accaagtacg
     4681 aaaaattctt taacgaccag atccatggtc agttagaaaa ttctgcactt ttttgcactt
     4741 cttaccttct ttaccgaaat tgatctttag tagtgtgtct tcttcgacct aaacatacag
     4801 acccatatct aatgttggtt cttttcaata ttgtttgagt tatcctacag acattgtggt
     4861 atcttcttta aatggataga gatctatcct gcagccttct accgcattag atttgtacat
     4921 atgtcgtttc atatcaattc ttttctcaga accacgtcag aatcaagact tcctactata
     4981 tgtgttgttt taagttctca gtattgcttc ggatttcttt tagatcattc agctctttct
     5041 ttctagtcgt ttttagacct aaggactcca attcgtttat ccaaccatat tttcctgggt
     5101 gttcgttttt tgtggctata atattcagac tatttcatct gaacagattg caatagaaag
     5161 ttctagagct aataaatcgt acttgacacg gattgcacat ggaagggaga aaagaattag
     5221 ttgtacttat acatgttcca taccgtttaa cgaagtatga agcgatcccg ggtcatcgtc
     5281 gtacagaatg cataccagat tatctcatgt actgcctcac actgcatctg ttagttacgt
     5341 tggatcactt tgaacctatg atatgtccaa tactgcatct gatatcagga gaaggtttag
     5401 tgttgccgat gacttccaag ttaaaagcat aacgttggtg tatctgcttt accttttcat
     5461 aattgcctcc atgtatttct actatgatca gacgaagttg tttcagcgtg ttcgttgccc
     5521 cgttcaatgc ttggacttca gcaccttcta cgtctatctt cattacgtga accttgcgac
     5581 cacttagaat attatcgaat gtgtcagatt ttatttgtat tgaatctttt tcatcaaata
     5641 cattacatat gctgattagc cacagtaact cgatcaccaa tgcgattctc atataatgtc
     5701 accttgatga tctgttattg ctgtgttgat tgcttctacg tcttgagata attacattcg
     5761 atatttttgc ataaagctct gtaggtctca gggtgtgctt cgattgctat gacgccaact
     5821 gatttatgct tatagccatt ggcaatactt aacgagtatt tgcctatatt gacccctaca
     5881 aaggtcacat attcgttcat cgccagacgt ttttcaattt ttttttctgc atttggggta
     5941 tgtcgtcatt attttttcta agaattgcct tgattccatc aatagaataa ggacgttctg
     6001 gtaaatattc tcctagggga aaagataaac gtttgatgga cagcgcaaaa aacatgattt
     6061 tgtaaatatt catctctttt cttcttacta tccattttaa tgcgcggtag tattcgaaaa
     6121 acaatggtat ttggaataag taaacaaaag ggaaagaata atttgcactt acactagaca
     6181 cgatatatta gtattcgttg gcacgaattt gattagaaca attgacggaa gaatagaata
     6241 gttactgatt tcctcaccaa atggtgtaca aatagcctat gcatcttgtt cgagcgcttt
     6301 aagtgtatat ccctgtaagc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnntg tgtatatccc tgtaagctac actggaaatc ggaaatatta tgcattgacc
     6541 ccccgtgcaa ttatttctgt ctccatggtc aatccgcttg cacattcata gtttgcatga
     6601 tttttatctt gacaatccta gttagagaag acggctgaag agtcattgta tctcggtgaa
     6661 aggccgttaa acggtagata atattcactt agttcctgtg atatatgaca tttgtccttt
     6721 ttcgttgata ctttctactt tgaatccgtg ggttgtcaat acttgcttta cgtccgcgga
     6781 tttatctcca tgtaactcta ctactatctt ccgtattctc tccaaagttt tcgttgctgc
     6841 tcttaatgct aaaacttccg caccttctac gtccattttt atcacatcga ccctataatt
     6901 aagtaatata ttgtctagtg tatcgcattc tatttctaat gatttcccat ttggatgaag
     6961 aatattattt gcatctatgc gttggtcatt gcaaacgcta tacatttcgg aatccacgcg
     7021 agttccatcg tgtgatcttt cgtacagatc gactagccct ttataatttg aaacgacctt
     7081 gttgatcggt atgatgacat tttcaaactt attgcattgg atatttctac ataatgcctt
     7141 gtaattttct ggatgagcct cgattgcaat aacctttacg accctatccc tgtaatcatt
     7201 tgcaaccatt aatgaataag aacctacatt tgcccccaca tctacaaaag tctcattttc
     7261 ttccatgacc aaatgtcgtt tcacttcttg ttctcttgat ataaagagca tctgaaagtc
     7321 gcgtgtcttg cgtcttggta tcgctttaac tccactatcc gttacgtaag ctctttcagg
     7381 tagaaaatca aactcggatg ttcggttttg tcgtaagaac ttgtcccgtt tatctttgcc
     7441 cattttaacg cggaggaata ttctgtatag aatgtaacga
//
LOCUS       DAOR01000057            7569 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35247, whole genome shotgun sequence.
ACCESSION   DAOR01000057 DAOR01000000
VERSION     DAOR01000057.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35247"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2195 a         1607 c         1325 g         2442 t
ORIGIN      
        1 cggtggacct gcgctgccgc gagataggtc gcgcgcgagg cgaggacccg cggcagcgca
       61 ggcttatgta ttcgtacgca ccgatcgtgt tatcaaaagc atagtgaatt tgctggaatt
      121 gctttctaaa gtcagcaaca tcttttgcta ctatgtgagg atcctgctta tcaacaacca
      181 cccgttttat gaaaccagca acctccttca tctcgctctc tttcattcct agcctagtcg
      241 tttcagggac accaattctc actccgcttg ggtgcatata gtgtctacct gccttaatgt
      301 ctccaggtaa taactgtctg ttcaaaataa tattggcctt ttctagatcc ttttcgattg
      361 taccaccatc accaaacttg gaaacatcca ctgcaatctg atgcgatgcc gtataacctt
      421 ttttttcgcc taagacacca aagccaaatc caactagccc ttctgcaagg gcccgagcat
      481 ttttgattac ttgttttgca taatcttttc caaactgtaa tgattctgct aatgcaatag
      541 ccttaccagc aacatgatgt agatgatggg aacttgtatt accgggaaaa actgctttct
      601 taattgtttc tgcgtgtttt tctaatgata cgatgattcc gccctgtggc ccccaaaggg
      661 ttttatgtga actcatactc atagaatctg ctccctcttt cagcgggtct tgaaattcgc
      721 ccccagcaat taggccagca acatgtgccc catcataatt gatatagata ccatgctcat
      781 gcataaaatc cgataattct tttacgggat gaggaaaaag aaagagactg cctccaaaca
      841 ttccaagctt tggcgatttg ccatcacgtt ccatttcctg gatcttcttt ttcgtcccat
      901 ctacatctat tgtcatttct tccttcgaaa atggataata ttcgatattt aagccgtgaa
      961 caagacctgc agtgccaaaa ttctcctttt tcccgtgcga aatatgtcct ccatttggaa
     1021 tagaagccgc tatcatatag tctcctggac gtgaaaaagc agcataaata gcaagattag
     1081 ctaccacacc ggatgtcgct ctacaatcgg caaactctga tctgaaaacc ttccttgcaa
     1141 gctcgttgca gattaactcc acctgatcga tatatgtaca accagcataa accctctcgc
     1201 caggccatcc ttccgcatat ctattaccaa agtctgacat tagtgcttct cttacggctg
     1261 cgcttggtat gttttcgctt gcgataagtg ggattgattc actaaaccag tgatggtgtt
     1321 ctttcattag tcttaatact tcatcatagg aagctttagc gttcaactta tgttatagaa
     1381 tcttccgttg ctagtcctaa tttaaaatat cgcattacag cactacataa aaacgcattc
     1441 acaaaaggta ttgagaaaaa ccaccaacgg agataaactc aatcctccgt tgtgtaattc
     1501 actagcactt ctcaagctac cgagacttca cattatcaat cacatttaca tttacattaa
     1561 agtctccaag gggagtagta gacatcaatc cttttcctcc atccatcttc gctgcgcatc
     1621 actgtcgtag gtttcttctt cataatccta gttttctgct acttgctcaa atccagttcc
     1681 tccgtcgtag tccgtcttgg aataccagat cttaaaggta aagtctgtta aaaaaagata
     1741 atgtattgtg cacttgtctt ctatccttca ttagaatagt aataacatcc gagtctgtat
     1801 ttgagtgagt ggtaccatgc caattcctcg gcgctatggt gtcactacaa gacagtacct
     1861 atagaaattg acattactct ccgtcctttg gtgatttacg ccttgtctgc tcttttatcc
     1921 atagttctat tgcttcttgt aacgctatac tgatatttcc tttcctgaag cctttagtat
     1981 tggccactgt ccttctaaat cgttcttcga tgtcatcttc tacgactata ttcattttac
     2041 ccacctgctc tataaggaga taccactata caaacatata gagtgaatcc ataataccct
     2101 acttactgct tgcgacaaag ttttcttgga agtatttttt aagtctattt gcatcgaaat
     2161 cacttatcaa tatgtcatca tggaagacat cctttgtccc tttgagtata ttacccttgt
     2221 ctgccagtat cttcaacgcg gaaggagtac cttttgactc tgaatgaact aattccattt
     2281 ttaatgctgg acctttagag aacgtaccct gttctccctc accacgaata ataaactcat
     2341 tacccttatc cttatgaggt ctgtcaaatt gcatagagta tctgttaatc ctgtcttcta
     2401 tcattactct caatttgtct actttgctgt ctttctctat cttgaaagta tacttctgaa
     2461 ggccctctac ttcaatgacg cttccaatag ggtctttatt attttccgct tcatgcaggt
     2521 gtgaatcctc tttagcagcc cagagcattc cttcatttgc aactgttatt ctaaatctac
     2581 cattatctcc gctaactttc agatgagaat caatatttct ttgcagttct gttgttgtca
     2641 ttgccttaga aggacctttg aaccattcgt ttttactctc cttaaaatct aataagccac
     2701 tatattggac aagatagctc tcctttgcat tccatctcct tacaagctcg tatgcctcaa
     2761 ttacagagat catccctgat tcgggatgtg ggttgtaagt gtgtgtgcct aagactaaaa
     2821 gatccgggtt ccacaacaaa ttctcgtctg cattaggtaa ggagaggaag tcccaaccca
     2881 taatgatctt tttgtcttga tacttgacaa cataaattac agagcttgga gtggaattat
     2941 caccgtgatt tgcaacaacc ggtgttaccg taaatggacc agttgtaaag ggcttttttg
     3001 gttctatgat gttaaaagct gatcgtcttt ctgccagttg aggaaatttg ctcataattt
     3061 ggtcacaaca ctctttggtg caatatacac tgaccttatc agatccgtcc acaaccttat
     3121 tgaaaagctt aggaaggtct tttacctgat catcatgaga gtgtgtaatc aggacagcat
     3181 ttggaatatc agatattatt atttttgatt gagcagacgt aacatcagtt ccactttttt
     3241 ccaagctctc aacaacacct tcacccacat caactagaag atgaaaattt gtgttcgcac
     3301 cgctgctatc agcagcagga ggagatgtgc tgaatataga acaagaagta tttgcaatta
     3361 tttcctttcg ttttatttct gcagctcttt cagatttttg ttcgtcatcc atcgtggaaa
     3421 tattcggtaa cgtcccatta attcttacca ataattgggt agtcttttct ggggtagtaa
     3481 gtgttctttg catattctat atatacacat atcgatataa atagatatcg atttatcaat
     3541 ttataaacat ttcaacaaaa acacaaatat gtataataac aagataatat atgacaaatt
     3601 cccatggaac gacataatcg aaaattcctc gcctcgccaa ttaactaaat agctgtggca
     3661 gtgaagcaac atgcctaaag actaaagtcc tccatttatt ctatctttca attgttatct
     3721 cagaaatagt tcgtcgattc ttcaatcaaa agcatctttt ctagatactt ttcatattca
     3781 ttcttgaccc aaactttaag tcaacgccat atttttgatc ataatcaaaa gtaaatgatt
     3841 cgttagataa tattgggaag cttgaaagtt ctgtagtcaa acaaatggga catctatctt
     3901 ccacaggcaa cataaactta ccaaagtacg ctgcacacca gaaacttgtg tcacataaaa
     3961 tgaatatgtt ctctttttcc tttgggtttc ctttttctgg gtaaaccttt tttgcagaca
     4021 tctcccctcg tgaattatct gccatcccca tttcagataa taaacagcat aattatataa
     4081 attgtgtcat aactgacatg gggaaacgac cgtgttgaaa agtttgctaa caatgggacc
     4141 ttcattacaa aaaggggttc ctttcgttca ggtgactcca agaagtattg acgaggacga
     4201 gatctagaaa aaatgtgtac gtaaccgata ttggtaacaa tcgtcttgaa aggccagtaa
     4261 cggcttaatt atatttcaat aggattcttc gatttaattt aggaacatcc gactgattct
     4321 cgaagacatg tggctttccc tgcgtgactg ccttcgtagg ttccaaacag ccatgtggtt
     4381 tgcccttacc atcctaggtc tggactacac accatttctt tcgaactagc ggttcaccta
     4441 ttattcctag tatgacaacc atgcatttcc gtggacgtac taggccttta cctaatacgg
     4501 aagtttgcat gatggatcat ccttctgttt cggtcagtcg gatatgatat ataacgcaga
     4561 acatcctaat atacttttaa caggacatag tctcttcgtt cggtattggt gttcaagaga
     4621 attatcttta atcgctgaaa ttcatcctcc aattcatgat taatcgcgtc tctcagtaag
     4681 gctagctcat ttgctgccac gatcatacgt caaaacatta ttctaaggcg tgtcccagtt
     4741 aagccttcgg gagcaaaaaa tgcaaccacc taagtaacta gataagtcac acttcgcctt
     4801 tggcgttaca tttagaattc aagcaatagc aataattcct caaaaatagt atcgttaaca
     4861 aatatggttg ttttttgtga tactctataa agagacttca aaggaaatgt agaggtttaa
     4921 ctactccact cttgccgttt ctttttgttc cgcgcttact cctcttagcc aaggatccca
     4981 gaatattttg aaccaatact cacccacttt tattgcattg tcatatgatc ctcgattcac
     5041 ttcgatccat tttgacaaat aattttgcca gaaaaccatt ggattgataa acggattgga
     5101 tccgtaactt gtttgttctc tatctgatgc catgacctaa tttaggttat aatagtagtt
     5161 aaagttaatg ccctccgtaa gcgccgtcta aatcatgtgt tttgctcagc ctattaggaa
     5221 aggaatgttt ggtttaaaat caaagccaca tacatacaaa aacatattta gccaactgta
     5281 cctcaaacag cctctgactt ttaatggaat gtaatgaatg gagaattcag gctttactag
     5341 tatttactgt tgttgcacta tcaagcgtca catcgaccgc atttttttta cccgctatat
     5401 catatagtca gttgtcccct acgccatcta gcaacaatat tgctaatacc aaagtagtaa
     5461 ttctatcttt tgatgacaac aggaaaggcg atttcacata tgccaaacca attttagaca
     5521 aatatggctt taaggctact ttcttcataa tttgcggcaa gacaacagat aaaggggcta
     5581 tgaattggca agagatagct tcaatgcaaa gtgatggaat ggatatagaa tcacacacta
     5641 tgacacacaa acatctaaat catttgtcag caaatgcatt gaattttgaa atagccggat
     5701 caaaacaatg cctcgctaac catggctata atacaatgag ctttgcatat ccttatgatg
     5761 aaggagcaga caatgtcact gtagtaaaca cagttgccaa atattatgat ctagcaagaa
     5821 ctggtagtga gccacttatg ttcttaaact gcaatggatt taagaaccat cctcagacag
     5881 actgtaaaac atatttacct gatggcaagc taacctacgc aaataggtat gctatcagaa
     5941 gtttgtcttt tgatagatac gaaataaagg acttatttaa caatgcctcc atattttctg
     6001 attttggaca catactcaaa ggccagagta attacaacaa aggtaatgga ataattagct
     6061 tgtctggaat agggaataat gtagggggag ctgttcctct aattacattt cataacgtac
     6121 gtcctgttaa caacgtaccc tacactacca atgtaggtgt gtttgctgaa ttaatgaaat
     6181 atctacacga taatggatat aaagtattgg acatggctaa tttaagatat gatacaaaga
     6241 gcaacgtatt tcatataaca tgataccttc ccttatagtt atcagttaat tcctatatga
     6301 ttttctattc ggtcgtctcg atgccattat tgttgatgcc atgatactct tacagagaaa
     6361 agatatgtcg taggaagtag atttttgttg ggattaagtt cgttgaggtc ttacgccact
     6421 acctatccgc atacagttgt cgccttccct cccactctct gcaagtagct cccatatttt
     6481 tagccgtcca accttttctt acaattccat atggcttgta gaaatctatg tgagaggctc
     6541 tattccaaaa ttgtttttgg taagagccat tgcagttagg acattgtttt ggtctcaaca
     6601 cctctgagac tgataatatt tgcgctgtgg aatcagcaat aggagaagga aaaccaatag
     6661 aagtctgtaa agattttctt taatatcatc ttttactaga agaacttgaa ggagaagtcg
     6721 tacttgtatt caatgaactt gtatttgctc ccatagtaga ggcagtagta gtagtaacat
     6781 aggaatgtga aagaccagaa tgaggttcaa gccatgcata aagatctgcc aacacatact
     6841 gttcgattgg tccaattcca gtcgaccact gagatgaggg atagaatgca tgtcccagat
     6901 tgggataagt tatcaacgta tggtcaggat gatttacttc agtcagtctt tgttgtaaga
     6961 gaaacgcttg ttgaactgga gtctgagaat cattttctcc attgagtatt aggatacctg
     7021 tagatttaga gacattacca atgatgctta aagttggtat caaactaaat tctgacctga
     7081 accacataga acattctgca tggttgcatt tgagcaaatt gaaagctgtg atgttttcat
     7141 atcgctttat cagcagaggc ttaagttgtt tatcaatact catgtaacca gcttcaattg
     7201 tattatttcc aaatttcttt actagagtgt tggttataat tttggtatta ttagtacgca
     7261 aaaaggtatg cacaacagaa gacggcacca ataaattcct taacaaagga tcctttgcta
     7321 tttgctgtat tgatatcaat ccagtacgat ttttatccag tacttgcgta gcgtactctg
     7381 agggtaagga tactgcttga tagtgcagta aatctcgaag gttctgagct ggtgtgccca
     7441 taagtattat gttctttacc ttcgtcgaat tatctattgc tactgttgga gcaataattg
     7501 caccttcact atgaccaatt atacttatcc gcttaggatc tacctcaggc tgttgtataa
     7561 gaacattca
//
LOCUS       DAOR01000058            3116 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_79449, whole genome shotgun sequence.
ACCESSION   DAOR01000058 DAOR01000000
VERSION     DAOR01000058.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_79449"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1232..1477
                     /estimated_length=246
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2728..2979
                     /estimated_length=252
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          772 a          471 c          447 g          928 t
ORIGIN      
        1 agattgaaat ttatctccaa aaacatattc gccgacatat tgttgggcat tggtagttat
       61 tccaaattgg ttgggattag tataaagaat tattccattt ctatccagca gtcctatagt
      121 actattaaat tgtggaaata tttgattttt caaaaagttg cccaacgttt cgaacctaat
      181 tgaggcaacc acaacaccag tgaatgttcc cgtactggta cgggtccctg tcgtattgat
      241 tacaggatat gatatgtata atctaggaac tttgtcattc gattctatca gactgctgta
      301 atatactgta tgagtatctt ttgggatggt aaagtatggt ctataactta ggtctgttcc
      361 tttgtatttt tgatatgtag tttcattaat attgctcaac caatttattt taccatcttt
      421 attgagccac atataaaagt cagtcagatc acttgaagaa tgttgtctac tgttaatcac
      481 aatgtctgct cttttgtatt cattattatg tatagcagga gattcagcaa gtgtttttaa
      541 aagagcacca atagtttgta ctttatttgc taatattagt gaaatgtcat gtacttctat
      601 tttggcattt gatcttactt cctgtgaagc aatatcacca attttgttag atgtaaaagt
      661 agaatattgg tatgaaaaga ttgatactcc caatacgaca gttgcaacaa cggctagtat
      721 aacgatatta gttttgctaa tacgctcttt atttagcaga gaagacacct ttttcttgtt
      781 gacacatcca aaaacccgca atactaatac ttgtactgat cattttactt cagccattat
      841 ctcttctata cgctccatga gcctttcgtt ttctataggg atttgtataa ggcagttagc
      901 aggtagagag gaaaatgtat catctgaata gtgttcagac tgccactctc cagctgtgag
      961 aaagcatgtt ttgacttcct tgtcctgtgc ctttagttct ctgtgaaaag caaacccatt
     1021 catttgtggc atcttaatgt caataattac aagatcatag taatgaggca taaaattctt
     1081 tagagccaaa tgaggatcct cataagaatc aactttgaat ccattatctt ctaatagttg
     1141 tcgtattgtt gtgttaacat cagcctcatc atcgaccaca aggatcctcc ttgatatatt
     1201 acttggccaa aagcctcctt ttccaattat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct ccttttccaa ttattcctaa
     1501 aatacctgcc ccttttcttg tctgctatgt gctcattaga gctagcttta cctgtttata
     1561 catacatggc tttattgtct tgtcaggtat actgcaggcc gtgaagtgcc aggctgcctc
     1621 aagtcctaat agggttccta cgaatccaat aacaagatat actagatttt caaacacatg
     1681 tatatattgt ttttacgctg ttattttagt ttggtaagct gtgtttatga taaatcataa
     1741 tgttcattat tttttaatgc gaagtgtaat tcttaattct cctaggaatt ttcagtaaag
     1801 cttctaacag catatctatt tgcgtacgtc aggctgccat caggtaaata cgtcttacaa
     1861 tcagtttggg gttgggtttt gaacccattg cagtttagga acataagtct ctcgcttcca
     1921 gacctaacaa tatcataata gttagaaatt aaattgacga ttgttttatt gtctgctcct
     1981 tcatcataag gataagcaaa ggttgtagga tgatagccat gaatgagaag acattgcttt
     2041 gatccaccta tctcgacatt caatgcattt gcagatagac gatttataag gggatgtgtc
     2101 gcagtatgag cttgtatatc cattccgtct ttttgcatta aggcaatatc ttcccaattc
     2161 atggaaattt atctcttgtt tcgttacata ttatgaataa tgtggctttg aacccatatt
     2221 tgtctacaat tggtttggcg tatgtgaggt tgccttttct gttatcatcg aaagataaga
     2281 ctactactta ccattattaa taccgctgcc gctgcttgtg gtagcagcga ggctatcaca
     2341 acatcgtgtc ttgaaggttg aggagtagaa ggatgacgtg gtatgatcag aggaagtgtt
     2401 agatttggct gttcctatct cttcaattcc gattctacag cagactgatc aacaattaga
     2461 ttcagcttca agtaaccctt gttgtgagac acaacaaaca ggagtagtag ttgtaggaaa
     2521 atagcaacac caacgacatc aataacagac aaacgaagtt gttttaattt tttgaggatt
     2581 tgatgctaga atggcagata gtaattagat caatcaaaaa aaaactatta tcctgttttg
     2641 acagttactc tatccctaac aagaaatccc aagcgtacat gccttcaata atagacttta
     2701 aaaatcctaa tctaactctg attttatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ataaaacgat ctaaaatcgt
     3001 ttcttctttt gattttgatg aaacttcttt taatgtttta taatatactt catatgcagt
     3061 aataaaccat gctttaactt tatcatctat ttttcttatc ctttcatata gttcaa
//
LOCUS       DAOR01000059            4021 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_26129, whole genome shotgun sequence.
ACCESSION   DAOR01000059 DAOR01000000
VERSION     DAOR01000059.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_26129"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1317 a          757 c          815 g         1132 t
ORIGIN      
        1 aactcagggc gtttgtatga ttaaaggctc tatgaatgac caagagaggg aacttttata
       61 tattctgtta ccttaaagtc tcttgctgag acagggaatg ccacaagagg taacaacaga
      121 gcagattttc accgttttat ccgataatac gtcggtaaac ctaatgaaaa cggcgtatgc
      181 aggaagactc acctcttcca attatgttgg caagctcagc aagaagcagt tctacaccaa
      241 actcaaacgc ttatgtaaca ctgggctagt cgagaagcgt gaaaacacct attatcgaac
      301 aactaccctt ggctcgctgg tatataatgg tcatgtcaaa acactagagg atgctctaac
      361 aaatttctgg caacttaaag ccatagatat tttgaaaaca agagaggact ttcctgcgga
      421 tcgtaaagaa tcaattatag acgaaatcgt taaaggcagt aatttgaaga actttataaa
      481 ctcgacccat ttaaccgggt ttgctataat caaggatttc aatcgtcttg tgattgaggt
      541 aatacgcatg cttgaagacg ctaaaaagga agtttatttc gcctcgcggt atcatgatcc
      601 acacgtttca aaaaagatgt tcgacaaagt cgaaaaagga gttaccgttc acttcttaga
      661 cggtaatcca cagcaaatca gtgtcgaaag ccgactgaat gcaatattga ggactcctcc
      721 caatgaggaa acatataaga cagttaatag gctaataaga tcacattgtt ttgagctaaa
      781 aagtcttgaa cctgcaacaa tagtaagtaa cggtaacaat agtacaatac ataaatttaa
      841 cagcttccta gtaatcgatg gcaaccaagt actttacgaa aatatcaact atggcaatcc
      901 ggaggagttc acagtagcaa ttgcaaggta tgacgatacg tatttggcag aacaatttat
      961 tagctattgg aaattgctct caaaggatgc tgtggttcct aagcttttgg aaactgcgag
     1021 agcgagaaca taaaataaca aggcataatt acattcagta accgttacaa tactatacgc
     1081 tattttaaaa atagcagaaa agtctgcgtg cagaccacta attaacgcct acgaattaat
     1141 attcagcgat aagtattctt catgctcatg tgcagacctg gttctagtta ttatgccgat
     1201 ggggtataca tcaaaaatta cgtcctagtc agccttggac caaaattcag tgtagagatc
     1261 attctttttt tgtcataaag tatccaaatt aggtaacaac tctgataaaa aggggcattt
     1321 gcatccaagt acaaatgcaa atagctttca aatctaggca ttcgactgct agctatggag
     1381 aagagaagag tcgagcttct ttagccttat cccaattggt tttactgcag tcgcgtaaaa
     1441 gatggggagt aatgtatatg ttagcgaccg tgtcggggct ttttgtaccg atagcaggag
     1501 tttttccctc tattgaagat cacggtgtaa attcattctt gacgacacta ctactaaaaa
     1561 gtgcaccggt actcgcggca acctttttca ttataaccgg aatgtatgtt ctcaatgatc
     1621 tagttgatgc cgatctcgat aaatctagtg gaaaaaaacg accaatctca tcagggcaag
     1681 tcacaaaggc acaggctctc atcttcgtga tagcgaccaa cgcagcaggg ataattctcg
     1741 ctctaataac gttcaatctc cccagcataa ttatcgcgct gacaatagtt ggaataggat
     1801 tgatgtattc tgtgcccaaa atttgtctaa aagacagatt tgtcctcaag accgcatcta
     1861 ttgccgcggc aagtatgctt tgtcttatgc taggcagttc ttatgcattt aataactctg
     1921 gtatgataca caacaacaac aacaacaaca acaacaacta caattacatt ggtgacgcac
     1981 ttagcagtcc aatcctaata tgtacctacg cagccttaat gtctggaagt ataatcttta
     2041 taacatcttt actgaacgat ttaggagatg ttgaaggtga cacggctttt ggtcgcagga
     2101 ctattccagt agtcattggc aagaagaata ctgtaattct gactataatt attgcagcat
     2161 ctatgattgt tatttcatgg gtatctttct acatgttacc gactaatatt gggttgattt
     2221 gtcccatttt ggtaagcctt gtcgcgtcga tggcatcaat aaaaatggca gaaatattaa
     2281 atcacctgag tgatcatgaa tttattcgga agcaacataa gaaatccatg tcgtggcatc
     2341 taatgcttca atcagcacta atagtgggag cattattatt ctgggtataa atcgcgctca
     2401 ccctagtgta atcaaataaa atgccaatca ctagaactac cagaattagt gtgtcgaccc
     2461 atttaatatt tgatatcata tggttttcat tattatttct gtacgctatg atagggggac
     2521 ctctccttgt tgcggattgt acctaaaata gggcattttt ctgtgtttgt acccacacct
     2581 tgcctcgcta agtctataat gtcgatactc tattataagt aggtgcagag atcggctaaa
     2641 ttaagatcta aacaggaaaa gaccgcaaga aacaaaaccc cgactgaggc taggattgca
     2701 aaaaccaaag gtattacagt agccaacgta ttgaagagca tttctgatcc catatctgtt
     2761 gaattattca aatctgtctc cgtcgcaggc agcgaaagct ccgctctaag gagcaagaca
     2821 aaactttctc gccgccagta ttattcaagg atatctagct ttacaagaaa tggaatgcta
     2881 gtaagaaaga acggaaggaa ttataggacc acatttggaa aagtagtcta ttatacgtta
     2941 atgacgattg aaaacgcatt tgtgaattat tataaactta aggcaattga ttcgattgga
     3001 ttatcctttg acattccaca ggaagaacat aaaaaaatca tcgacaacct gataacggat
     3061 cccgaaatta gacaaattct ttcgactaga agaaactgaa agtctaaagc tttttctttt
     3121 tattatggct aaaaataata tattgtcgca cgtttttaca caaacgtgtc gataaaatag
     3181 cttgaaggca gtattgcttg caggtggtca cggtacaaga gctagacctt tcactgacta
     3241 ctttcccaaa gcgatgatcc ccctcaacgg aaggccggta atacattatg tcgtgaggta
     3301 tctggcaagg ttttctcaaa tcagtggttt gattatattg tgtgagttcg ataattttgg
     3361 caaacaaatt attaattatt ttgatggcaa agaagctgcg atagggaaac ctttaacgtt
     3421 tattgaagac aaaaagtatg ggacaggcgg tgctcttcta gaaattgaaa gagatgtgaa
     3481 aagagataaa tgctttttgt tatggtttgc ggacaacctg tgcgccataa gaatcgatgg
     3541 tttggtacaa gaatatgaga aggcagacag aatgacaaag ggaggaatta caggaatcgt
     3601 ggtcgttcga aaccaaaggc gtgaagaaac gggccgagtt attttagaca acaccactca
     3661 tcgtcaggct gctaacccca ggaagcctat gtcatcatcg atcaaacaat ttattgaaaa
     3721 gggggtagtg aaacttgagc aaccagaggc acttggcata tatctattta acaacacaat
     3781 ttttgattat ttacacgcta catcggagaa aatgaaggca ggtggcttta atttatctca
     3841 cgatttattg ggacgcagta gtactattcg cggcaaactg tattcatata atctaggtaa
     3901 tacagattgg ttagatgtag agtcaccgcc atatgcagac agaaacaaag atactgtaat
     3961 gaagatattg tcgcagatgg gtatgtagta tgtatgatga cactgcagcc atcactcact
     4021 c
//
LOCUS       DAOR01000060            6144 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_66590, whole genome shotgun sequence.
ACCESSION   DAOR01000060 DAOR01000000
VERSION     DAOR01000060.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_66590"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1936 a         1125 c         1116 g         1967 t
ORIGIN      
        1 ctcaggtatt actttcttac tacgaggttg ccattcatgt ttacacctct cacatttgta
       61 acctttaact ttgatttcaa tttcaggtgt catcatatac catatggtat caaacctatt
      121 attaaatatg cataaaggta ttctatacaa acaatatgaa tatatcccat atgtttatat
      181 accgtaatgt tattgttttc ctatagacat caaaaggatt tagtagacgc acagaagaag
      241 ttagttgagg aacaaaagaa tttagtcgaa gcacaaaata gacaattgaa agtccaagag
      301 gaattggttg gcctagaaaa gatacaaaat caacttgctg cattagttga gatctttcaa
      361 cttctggacg ataacgcaca tagaaatgca agaagacgaa ttgttaattt atacaccgat
      421 gattcggaga aacaaaagtg gggtactcta atacttatga acgcagtgaa gaaaggcact
      481 gagtataatg aatacttttt acgaagcaac ctatatgggt tttccagaga tagaccaaag
      541 aatgctatcg tatctgagta aagagggcaa aaagttcttc tggaacttag aatatgcttt
      601 aaatcaactg aatgtaatgt ataacataca tccaagtata gaggaagtag aacggattga
      661 cataactaaa ataatttatt ttgatggttt gactaaacat acatttttca gttcgctgtt
      721 cgatattcag aggggaatgc attgtgtaaa catacaatat caatggttga gtgagcaggg
      781 atttcaatga gatatgtgcc actttttgac aatttactcg tattccacac aatcgacagt
      841 gtatgatgta tttggccgta taatgataca cataaagtag aagagcaaag aaaccgaaag
      901 agatgaaatt aaggaggaaa ctcatgaatt aggagatgag gcgtagaacg aatcagaagg
      961 cgaagatgtg gaggaataga attgaaaact ttcagtgtgg atgtatacca aatcgcctta
     1021 tctcacttct accttgtaga aatttgcaat acctttctct tcttccatag ttctttctag
     1081 ttatctttaa tctcgaatcc attctggata gggcgcccag tggatacaca tttctagtga
     1141 gttattatag agatgaggat cgagttaata gtcgcattct ttgggaaatt gttctctcca
     1201 atttctctcc ggttaacgtt tgtccatggc tctgcgtatt ccagttacct ttgagtagat
     1261 ctggttacat ttttgataac atttacctag tctgtcgaaa acactcatta aagcactctt
     1321 cacctctacc cttcggaata gccaaagcta acatgcccca agattctttg tagctacaga
     1381 gctctagagc aagaagaaaa atggcagcct ttctaggggt attgactaga ctagtagttt
     1441 tctctagatc tgcagacata aactccttac cttaataaac gacttgtagt acacagaagt
     1501 tttaaaggac ctaagatgaa tcatgttagt ttgctagggc tatttatggg aagtcatagt
     1561 ccaagtcaaa tttggtctat atatagatgc tactaagctt atgaccgaag ggctccacgc
     1621 aatgtatcgt tgaaggtcaa cctaagcgat taggagaacc aactaatttt aaattccgtc
     1681 ttattcctta gacttaggaa aagacaacaa ctcgataatt aaccattctc aacttgatcc
     1741 ttaagcaaga gctgtgttta cagcacttac aaaattacca attgtaacag gtttttttat
     1801 aatatcatct gtggtcttta catcaggtaa tatacttata agctccggta cagcatctaa
     1861 tgcagataca aacagtatct tgatctttct attaattgcc ttcaatctat aatatagctg
     1921 taatccgttg aggccaggca ttcttatatc taaaatagcc aaatcataat gagacggatt
     1981 taccatggca aagtgtgcca atgcttccat aggatccgca aatgcttcaa cattaaagcc
     2041 ttcagaagct aatcctgtct tgaaagtgta gagaacgtct tcttcgtcgt ctactaacaa
     2101 tatagttgga gtatgtcttc catcctgctt cctctcagac tggctaaaag tactcctatc
     2161 attgtgttgc aaaaagaata caggatactt gttaaattcc ccttgctggt cttccgatct
     2221 taacatctct tcgaaatgat aataattttc ttcaagggaa gtaagcaaag agaaggatcg
     2281 tatgacttta tgtagggcat ctcctatcgc taacccattt ggtgatgcct tcgagtttat
     2341 ctttgcacac atattcatag tgggaccgaa caaatcatcg ctttgagatg tagttgatct
     2401 agcaacttct actctaccat aatcagcgct aattctgtag ctaagagagg gtaattcttc
     2461 ttcttgcagc ttttcattaa ttgtatcacg agcttctatc atcgttatac aacattcgat
     2521 cacatcttta aacgaagact tgttagaaga atctgatgtt tgaggataat agaagatcaa
     2581 acaatcacca acatttttta ttactttggc tccaaagttt ttcgctattg ccgccatcgt
     2641 gttaaggaaa atcccatagt atttcctaac tttttctgga ttattaatac ttgaagtaat
     2701 ccttgtagac tcgactatat caacaaaaca aacacaacaa ttttgcgatc taatgaaaga
     2761 tacttcctca gtctcagcag cctgactatt ttgatccgaa ggatttggat aattagttgg
     2821 agtttcattt gctgctggca tatccttcaa gaacaagaag gagctagtgt tatttaagcg
     2881 attgaatttc tatataatga taaaaaattg gaacctgatc tggagtaggc agacattatg
     2941 aactcattca ggctcacata aggtcggtgg tccagtgtag catactggca aaggaccaac
     3001 atctacctta gtctagtcct acaggtgatg ataatctaca aaataatgaa tgcagatggc
     3061 aatttatata ataaattcaa cgaattcaat ctatcagggc agaatttcgt ccattctgtt
     3121 taatatcata ttgatataat cagttgtaag caacgatgcg gatagattta gctcatctgc
     3181 tttgagaagc atggaattta ttcgacaaag gatctcaatc ttcctgtcag tacatggctc
     3241 ttttgtgcaa tcctcaacga gttgcaatat tatactctga tacgctcgtt ttctttttgt
     3301 tttaacatat tcgagttcaa aagtatatga tgcatttgat ataatatcta ctacagtaaa
     3361 agctgattat gatttatcat aatggaaaga actgttacga ggcttcaaac acttaccaat
     3421 gacatactgg atgctggaaa aacaattaac cacccagcac ttaagctaaa tgtatctcct
     3481 ttaccgtatc ttgcccctgt gagcttctca aatgcctttt agaaataaac aaccctaagc
     3541 ccgtgcctcg aggcgaattt gtagtaaatt ttgaaaatag tctaggcata atctctggat
     3601 ctattcctga acccgtatct tttatactga taattgcttg gttgttctct tttctacctt
     3661 aactacaatg cttccctctt tagtggattt accagcgtat tcgactgcag gctattcata
     3721 gcaatgtttt cgaaacatca gatgatccct tcagcttgcg agattggcag tgagatatcg
     3781 atttttacct gcgatattaa ttgaatttcc tagcgatgat cctagagcac caaaactgtc
     3841 aataattaga ataatttcga tgacaactaa gatcgtgatt tttaaaagaa tcttagattt
     3901 tattcttttt aaaaagttaa tcacctcctc ctttacatcc catatcctca gccattgaag
     3961 gccggtgtct ctattcttca gttcgtcgat cctcgttgtg aggctggctt taaatatttt
     4021 atttaatccg ttagtaatgg atgccattga agttttctta aagaaggttt tagcgcggta
     4081 ttagatatgg cgacacttca attataatta atgttgcgac aattacggcc aatataaaaa
     4141 tgtgttcacg tgttaacttc atatcatcct cccattattt aggcatataa aactatataa
     4201 tcattagatt tagattgttg gtgttatgtg aaggtgtagc ttatgtgcga ggttaaagat
     4261 cgtatcatcg gcaagttaca gtcctcttct taaaccgttc ggaaaagaag caacggaagg
     4321 atcgattgat gaagctttta tatgatttgt ttctattgac tggttgtaga ttgactgttt
     4381 tttggcgatc ttttctgcca agcaaaaaac atgaatagga atggaatggt catgacggcg
     4441 gttgtaataa gaataggctg aaatgatgtt ggatcaattt tgattccaaa aagttgtaaa
     4501 aaaggaagtg gaaaaactgt atatatccag aagccagcca gtattcctaa aataaactta
     4561 tttcgattta gaactgaaac aaagctaatt ttatttattt ttcttcgatc gcaggtaatg
     4621 cattttgtag tagtataatt accagtgacg tcttctggca caatacacga cgtacagatt
     4681 ttgctttgac aaaaataaca ctgcctagtt acaaattttg atccgcaaat agcacattgc
     4741 gattccatgc tctcccataa aacgtgttac gcgatgagca tattaaaaga ttcgtatatg
     4801 gtcccagtac gatgaaagca acaccagcta aagataagta catgtcagtg ttagacgttt
     4861 gtgcatatta tagactaact agaactggat gcttcctcaa acttagagtt aatgacttat
     4921 ctgcattttc agaatgtttc aaaatatttt caaaattcaa caaatttaag cgtgatttgt
     4981 aaataatgtt tcaaattatt gttgaggact acacttatac gtgaataatg tctgtttgat
     5041 gaaactttga atatgggtac cgcaggagaa gcaccggata tgttcttcct caatagaaac
     5101 gagaaagcaa aggacaatgg taacaaaact attgccaaat gtaagcactg cgctatggaa
     5161 tttgatgata gagagaggtt gagcgtccat aataagaagg ctcattcggg aaggggagaa
     5221 aggaagaaga aagcttaact aaataaaata ataggaccga aaaagatata tcatatgctc
     5281 aactcttccc tccacgttta gcagatctta ctttacgcta ccaccacctt agtcgatttc
     5341 ttgctaactg cgtggtttga ccacgtagac tactcgaaga agagtttgac aatttgatag
     5401 agataatttc gaccactgtt ttccatcctt tggggtaact tttgcggaac tacaagaggc
     5461 ggttgctgtc ctgattttat gacatgacat ttttattata ttatagacag cggcgctatg
     5521 cacttgcact cgcaaatgta attgtagctg aagaattgtc aagacaatgc cccgcagatt
     5581 acgatctcct ttaaatcgtt ttatcctatt atcaagcgaa tggacagata tcagaattat
     5641 cttcgtgtgg tttaggtctg tgactctaat tccttcaatt tcagagttcg aaggtgaatg
     5701 tggaattcaa acaccactag attgctcctc cgccgatccc ccgatcttct gcatttggaa
     5761 ttgtaataac tcgattagtg gatgacaaga ttccagtttt ttatccagag aagtttcaga
     5821 gtgatgacct actcgccact ttgagtggga gagtataata tctgtaaaga tatgcttccg
     5881 cgtgaactaa tgaacatctt tcttaacatt aacttataat ttggactatc caaactatgg
     5941 ctcatttcat caattacctc tttcattagg taattttctt tccaacaata gttccagctt
     6001 gaattatagt atttggctga acttgtctac ctggtagtat aattactgaa gccccgacag
     6061 tacaattatt accaaccact gctccaaagt ggtcggttcc agtatctacc aaattatcat
     6121 caatttgata tcttacgttt tttc
//
LOCUS       DAOR01000061            3547 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_16317, whole genome shotgun sequence.
ACCESSION   DAOR01000061 DAOR01000000
VERSION     DAOR01000061.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_16317"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1028 a          735 c          622 g         1162 t
ORIGIN      
        1 atgcccctgc tgattgggtg attaatatac catctggtga attcttctat ttccttattc
       61 tgtaggttga gagtgagctc cataacgccg agctcattct tcatatgctg ttctacgatt
      121 tgttttaatt catttagagt aagcacgcta catcaaaaat gatgtcctca gctttatctc
      181 ttctaaatgg ttactttcgc gggttgatcg tccactgcag taacgattgc aattagtgaa
      241 atcaagataa tcgaaaactt gctaccactt actgcgctag gtatagagag caatattccg
      301 gccgcaatgt catagaatca atggaataaa tacatttatg tcgccggacg aaatatgctt
      361 acaacacgtc ttgaatgtga tacgttgctc tagatacagc atcacaggag ctaaatattt
      421 ccctcagata tcttagccga tttcacttag aagagttgaa gtcgtactag tattagtagt
      481 agtactagta tatgcactgt ataagtcgct ttatagtttt tttgcctcaa taaaccatga
      541 tttcacgctc aattatagag ctaatctcga taaaatcaat aaaaaagtgt agcccaattt
      601 ttatatcctg taacctaaat cgaccaagag ttatcccata gaagcaataa tatgatacac
      661 tactatcatg attgcagtgg aatatgataa tgtaaatttt ctctatttcc aaatgaagag
      721 ctacttacca aacagaggtt tcgcagatag ctgttggttt atttgcttgg ctgatttttt
      781 cttattgcgc tgaggcgagc tataagtact cgcacatcta tgtctttaca cacaattata
      841 attcagttgc ctcttcggta aactttctct aatccacaaa caaactctgt ttctgatttt
      901 accaagtttt tctatttcaa cttcgactac atcattatgt ttcagatatt tccaactatg
      961 tgtagacaaa gacaatgccg tgccacttgg ggtgcctgtc gatattatat ctccagcctc
     1021 aagtgtcatc accttgctta ggctatgtat tatttcatct acctttagca caagattttt
     1081 ggtagatgaa tcctgtctga cttctccatt tacttttgtt gttatacgaa gattgcaagg
     1141 atcgggaatc tcgtcactgg tagttagcca cggtccacat ggcccaaacg tatcaaagcc
     1201 ttttgctcta gtgtactgct tatcaataaa ttgaacatca cgtgcggaaa catcatttag
     1261 tacaaaatac cctgcaacat aggttggtgc atcatgccta ctgactttac tacatttctt
     1321 gcctatgatg aaggccaact ctccctcata atctagctgc ttcacaaatc ttggacagac
     1381 tatgtcatcc attggtccga taaggcttgt tcgtggttta atgtaaataa caggttcttt
     1441 aggaggcgtt tcaccgaaac gtatccaagt agattgatct gtataattga aagccaagca
     1501 tattatcttg aatgatttta atattggtgg aagaactttg aattcgtcta agtcatgaat
     1561 aaatgataac ttgctcagat gatcttttat cttatgaaga tattcaccaa acagaaattc
     1621 ctctaagtca gatggtacag ctaatcggag ctgtcttaca atttcgtccc tcgtgatagt
     1681 cttcccttct tcattgatga tcgtaaatgt ttcatgatca cggtttagaa ttcttgctac
     1741 cctcactgga aaggatctcg atgtagtaat atgactacta cccggtatta aggtaataat
     1801 tataagaaaa atattaaggc tctctagtcc tgtcgagtaa tagtttcaag attcaagata
     1861 gtcgtgtgga aacctcataa agactgctcg gttattttta ttctataacc tatatttccg
     1921 accaaaagcg tacagagcct gcttgaacgc ttcaagtgga ggtctcacta ctcctgcccc
     1981 tatcatacca atcccggctt gcttatgagc cattgcggta tctataactg gcaaaatccc
     2041 tgtctttacc acttttctga tatcgattcc ggttgccgta ccttgaaaat caagtatcgg
     2101 aattgaaaaa gtgtcattca gagtcattgt tatttcacgc atttgtttag tgtatttgat
     2161 agcatcctcg gcggaccctc ctataagcga caaaattgca ggcgagttgg ctaatgcaaa
     2221 agcgccaatt cctgcagttt cagtgatagc actgtctccg atgtcaggat ttgcgtctcc
     2281 atgtttatag cctgggaagt atcttccctc gatcatctga gcaggtccta tgaaccactc
     2341 atttcctagg ccgctgactt ttatgccaaa gtcggttcca tttcttgcca tcgctataac
     2401 aagagtacta tactcgatat cacgtgcggc attcattatg gttttgcaag cagccatgct
     2461 catgcaaagg aaaaagatat catttgtcga gaaaaatttt gtgaccttga ctagttcttc
     2521 tctgtctaat acttctatta gataaggcgt aattatactt gcaaacaaag cggtaccagc
     2581 gacaggccta ttatgtaatt catcacccat atgaagtgct ctagccatta acgatttgag
     2641 gtctattccc ttactgtacc gtattgcctt tccaagtgcc ggacctaacg ttttggccca
     2701 aaattttaga atttgaacgg catcttcgtc gaaaactcca aactggactt tctgttctac
     2761 aaggcggccg aaacacgtat tcccaaattt ttcattcttt actaccaaga cgggcattga
     2821 aggagaaatg acgccagcca taggtccgac ggcatcatgg tcatgatttg gggaaaactc
     2881 aatctgccca ctttctatca atttaacagc ttcatcccaa ctttcaacta actgttcaaa
     2941 gatcatcgta gcgactatag cacctctcaa cggaccactc attcttttcc actcgattgg
     3001 cggaccagca tggaatattg aattcttttt catacctgaa agaacttcta ttgctggttt
     3061 tatgtcaaca aggataggtc tggatctgag aactctttct actgcttgtc tgtttgcatc
     3121 ttcgatcctg ttttttattt gctcactcat tttctttttc acttgtcatt agagtttcat
     3181 catctagacg ggttgatacg aaaatcaagt ctcgagacct tgatcctatc gttgagttaa
     3241 aaatttaagt ttaacttatt taaagtttgt aaaattacat atataagaaa attttaaaat
     3301 aaaataagat ttgattgttc acatctttta ccaaatatac atataatgat cgcctaaagc
     3361 gtgacaataa taagtatgac aataataagc ataataataa tgctgaattt cgtttagaac
     3421 atctaagcag tttccgctct tagatagcaa acatgctact caaaaggtct attcggtttc
     3481 atatcagtaa tatgaataaa caaagcatac gctgcgtata gtatattgtg tgacggaaaa
     3541 aatttaa
//
LOCUS       DAOR01000062            9211 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_44425, whole genome shotgun sequence.
ACCESSION   DAOR01000062 DAOR01000000
VERSION     DAOR01000062.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_44425"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    240..425
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1962..2216
                     /estimated_length=255
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2459..2808
                     /estimated_length=350
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3114..3384
                     /estimated_length=271
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2364 a         1631 c         1593 g         2561 t
ORIGIN      
        1 tttaatccaa ttcttctcca gttgctcctt gatttcgtta gcggtaggca catcctagtc
       61 catatgttaa agcagtaaat gattatttga agatatatta ggcaatcgtg aattggtgcg
      121 tacgatctgc tccttcttat ttctcgaagc atacagaagc taaagacaaa actggatatg
      181 caacccgata atttcagaaa aaagataaat gtaacacatt cgtaggctag ctatatctan
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnagcta tatctacagt aacaggtcta cagcgacgat cgaaggttag acaagagttt
      481 aataccttcc aaagctgatc ttcagcactt aaacagaaaa gggatgagtc ttacagtttc
      541 aaacactcct aatgagtaga tttatgtata tgcgtaaaaa attatgtcct acatgtagta
      601 aattttatta tgggaaggat ttacacagca agttacatga tcttggatat tatcgggttg
      661 gttggtggat gggctggaat aaatgaaatg taagaattgc ggagctgagt atcaagattt
      721 tggatgtgat tgtgaaggtg gttcgatcac ttgtccaaga tgtcaccagc aaggaatcat
      781 gagaaagtca taagcgataa ccatttattt tcaaacccca agttccctta tgatataagt
      841 tgagcaacct ccaagttttt gaatgtctca ctccgacctg taaagaatgc actggatcat
      901 atgttggtat gggattaaga ttagtttgcc agtgctcttg tcattcttct aaataattcg
      961 gcataactga gaattgaatt ggttgagctg aagtctatca aggttacaaa gccaacgtat
     1021 gaaaggattg gcaaggaagt atcattttat ggtgaaacta tggattcaat aattatcagg
     1081 ttattagatg agcttgaaga atgccgaagg aaggctaaga aatgacactt tcagagtatt
     1141 gaaacctatt acttgaactc ttatgccagt atcaaagtaa gtactgaaaa agggatgcag
     1201 ttggaaagac aataatgtta acgagtacag tcacaaaggc atctatggct ttattactaa
     1261 atctcagaga gctactataa ttattgtttt cagtattata ccctctctgt atatttaaat
     1321 atactagtct gcttcaacgg atgcagtttc taacaggttt ctgggagact tgactaatac
     1381 atatctgact ccacataaca taagtttggt gtcccactgt taatatacct aaatccactt
     1441 atgtatactc acgatatgaa tagacaaaca aacttaagag gtagaaatgt tgtgttgata
     1501 gcagctgcta tattattagt tagtatcggc ttatccacat cgcctattgc atccacaaac
     1561 gacgcaaacg ctcaggcgaa cagcaacatg actggcagta tgacgggagg tagtagtagt
     1621 caaaaccaaa ctagttttgg accaaacatc acaggcactg ttgccttagg tccgacaata
     1681 gccaaagcga ttgcatcaca agttcacgtt ggtctatcta atgctagtat tattgcagaa
     1741 aaagcggtgg gtcctaacgc acatgcagcg gcagtcagaa ttggcgtagt gcatggattt
     1801 ctagtatata tggcattggt agtggatagc actaacaatt tccatggggt tatagttgat
     1861 gccggtaacg gaaaggttct agcttctact cagatgtcta ttgcaggcat gatgcgtggt
     1921 ggtatgggaa tgatggaacc aggcatgatg atgggaccag gnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggca
     2221 tgatgatggg accaggcatg ggtatggtgc atcatggtat gatgagtaaa ccatatcctt
     2281 aacgggaaag atgtagcaaa acaacacaat caacccataa caatcctttt ctttcttttt
     2341 ttaaatcggg ctaattttac ggcgtacaag taagacaaaa aacagtgatc ggtgaacaca
     2401 attctaaaac ttacttgaag atatctctat tatctagcat gtaataagaa tatctaagnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aaaaatacaa
     2821 ctatgcccct caaactatgt tatcggtatg ttgcttgatt ttggaatcgg aatcttgagc
     2881 gtgataacac cgtctacata tttcgccgtc ccaataactt tttcatcatc gctgatcgaa
     2941 aatggtaacg gtactttttt gtctatgtag ttaggtctct gtcggtaata tacggttccg
     3001 agattttctt ctggctctct cttggcgttg attgataaaa catctccgat tattcttaga
     3061 ttaatatcct tctttgcaaa tccaggcagg tcaatcgtga ttattaactc gttnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnctcctt ggccatgtac tgtgcaaaac ccatagatta
     3421 aactagatgt tattgatata aatcctttct tcctaaattc taggattctg tgcgcggtac
     3481 ataaataaat atatgctact ctattcatct ctttgctcaa gctacattcg atattacaaa
     3541 aaataagaaa aagaagaaga aaacccagct gactttttat cagcagaata tctacaatcc
     3601 gatttacttt agaaatatca ttccccaagg aagtagatag tgttctcctc aagggacgat
     3661 tagtatatgt gtcactttat ctacgttgcc tagttaattt ctttagatag tcttataagt
     3721 tgtgtataat ggttggaaat ataatggata tgaagggtcg gtttgcagca taacacaagg
     3781 aggaattaca tgataggtgg ttcaaaggat cggcactatg ctcgagagaa tcaccaatca
     3841 atgtcatgct ggcttattat cttaaccaat tcagtagttg taatacacat tattctaaca
     3901 atttctgcaa actttgagac tcgatgaata aaattctttt agaaagaaac aagaataatc
     3961 ccctcctcat tccagataaa tctaattggt gggaatccaa agccgcattt aactgtgcca
     4021 ttctgtgctg tgataataga attcacatgc tttatagagc gataggcgaa tatgaaaagt
     4081 atatctcaag aataggatat gcttccagca cagacgggat ttggtttgat aggtccaaag
     4141 acgctgctat ggcacccgaa gaggagtatg aaaaatacgg gatcgaagat ccgcggctgg
     4201 tagagataga tcgccagata tttttaagtt atgtgatact ttcggattac gtgagcgagg
     4261 gcccaacagc atctactgcg ttggcaacta ctactgattt taagaattat acaagactgg
     4321 gcatcataac aagcaaagga tcagacaata aagatgttgt tcttttccca gaaaaaacaa
     4381 atctaaaatc aattaatgca gaggaaaaag gaggaaatac aaataagtac ttgtttttac
     4441 ataggccagc cagttggatc ggttcaaaat ttggagtaga tagacccagt atttggcttg
     4501 gggagggtaa tgcccttact aattttgaaa aacatactct acttctgaaa ccggagcaag
     4561 aatgggaggc attaaagatt ggagcaggac ctcccccatt aaaaactaaa gatggttggc
     4621 tgttgatata ccacggaaaa gatacacagg ctgtttatag ggctggcgcc gcgcttcttg
     4681 atctcaagga tcccaccaaa gtcctcggaa ggacaaaaag acctattctc gaaccgttag
     4741 agctatatga aaagaatgga gacgtaagca acgtggtttt tcccaccggg gcttgcacca
     4801 tcgacggtac attatttgtt tattacggtg cagcggacaa agtttgttgt ctggcaacga
     4861 tagacctcga aacgttgctg gattacattc tagatgagaa ccatgtcaat agttagatca
     4921 atatagattt atgtcgcctt cgtaattaat ttacatgact taacctcacc cagcctcgct
     4981 tacattatat ctttcataat agtggactag tgggaggggt acgacgatta aatttccatg
     5041 cctcacaagg caacaagcac atttttttgc aaacagactt tgcttcttca tgctcacaaa
     5101 ttggacacaa acccggctgc ctattgatat ctgtaaccga cttctgttgt ttagattttg
     5161 aacccacgtt taaccagagg gcatctgagg gtaagcctac aacaaaaacg acgatgataa
     5221 ttagtatcgg aataagttac gtgagtattg gtaatacgaa tctgttactt caaaacacat
     5281 tgtacccgca tattatacaa taaaagaaag ccagcgcgtc gtgatgccca tatcatgtcg
     5341 tgcggggtat taggaacgtc tcttgtttaa gggtaatgat atgccgatgt tcattgaatt
     5401 gggaacggtt ctataatctt gcctctgagg ctagtttgat tgtaatttct tcgccatttc
     5461 cgattggtac tgttactgac tgcagggaag gtttactgcg aacataattt gcatacttcg
     5521 ccataagagg ccggtaatct tcgggataca acatattgtc cgttgcaaca atgcccccgc
     5581 ttcttagcat tggaagaacc aaatcaaagt attctctcaa atactctttg tctgcatcga
     5641 agaatacaaa gtcgaacaag ccgtctgttt ttttaatgtg gttcttgtct tcgaaatttt
     5701 tcaatagctc atgaagtatg ttccttgcgc taccctcaag cacttcaatc aggcctctga
     5761 ctccagcatc atcaaaattt ttagtggccc tttcgatctt tgagtgattc atatctacag
     5821 tgataatatg tttctcttct ccatctggtc tagtcgtacc atcctgaatc attgcttcag
     5881 caaaccacag tgtcgagtaa ccaacagaag ttcctatttc tagaattctc cttgcatgca
     5941 tagcactaag gagtatttta aaaaacatcc cagtatcagg tgtgattgca agcatctcgt
     6001 cttccggaag aacctttatc tctccagaac gctcgcgttt ggattgttct ccaagtctgg
     6061 caagaacact ctttatgttc ccactcacac gactacataa gctagtatag tcatttaaat
     6121 ccacaggaga actcatgtgt gcgccggttg ctcacggctt ggccttttca aaagaacgta
     6181 tagtagagag aacttctaca ctcaacttta tagtgtgggc accggcatcg tctgagttgt
     6241 aatgagacaa ttgttcaagg aattttgact tgtctcggcc ccgctcatac atgccacctg
     6301 actccttaat acagagtggg aatttttgca ttttcactcc tttagatttt aaataataaa
     6361 tgaaatgttt gtatgcctca acatcatacc agtcccacgc cttctcttca cagcacatta
     6421 tccagacatc tatggtattc tggaagagag ctttggaacg aaggtcttca agactcatga
     6481 tatttcaact cgggttctct gtattttgaa tctatcgttt tagcctctat ttatctagga
     6541 attcatatac ttaaacaaga acagcagaat ttgaaattat tacctgatgt atggttaaat
     6601 tccaatatca aatttttgtt gtcgccctct tcgattttgt gctctcgttg attgtagcga
     6661 gatgaataac tactgggtga gggagacttc aatgtaaacg tcatcaggta ttttcaatct
     6721 cattagctgt cgtattgacc tgtcatcagc acctagatct attactcgtc tgtgaatacg
     6781 aagttcatat ttgtcataag tgtttgttcc ttgtccacac ggcgatttcc tggtaacaac
     6841 tttcatcttt ttagtcggga gaggatgcgg acctttcaat ctgattccat ttttttctcc
     6901 aattcctttt atttctgtac atactccttc aagagttgat aggtttgtac ttgtaagctt
     6961 tattcgtgct tcttgtggca tcttgtttca cttattgttt ttttgacggg tcatatttct
     7021 ctgtaatcga tttgactacg ccggcagcga tagttgtgcc catgtctctt aaagcaaatc
     7081 ttccaatctc tgggaattct ttgaaggttt caatcgctag tggccttact ggttttatct
     7141 ttacaattgc ggcatcccca gtcttcaaga actttgggtt ttcttctacc gtgcctcctg
     7201 ttttcggatc aatctttgcg aggaattctg atagagttgc cgctacttgt gctgtatgtg
     7261 catgcaatac tggcgtgtat cctggggcca tcgcagtagg atgatggatg acaatgattt
     7321 gtgcctcaaa ttcctttgcc accgtaggag gattatcggt tgggccaatt acatctccac
     7381 gctttattcc cttcttatct acgcctctga ggttaaaacc gatgttgtca cctgcctctg
     7441 ccgagtccat ctgtgtgtgg tgagtttcga tcgactttac ctctccagta atcccggagg
     7501 gcataactat aactttttcg ttagccttaa ccttaccggt ttccactctg ccaacaggaa
     7561 ccgtgcctac cccagttatt gagtacacgt cttgaacagg gattctgagt ggttttccta
     7621 taggcttttc aggaggatca aacatatcca aagcatcaac aagagtgggt cctttgtacc
     7681 acggcatgtt ttcagacttc ttgatcaggt tgtctccctt ccatcctgag accgggacaa
     7741 aatttatttt ggttgtattg tagcctacca actttaacat tttttcgaca atctctttca
     7801 cctctttgaa ccgagcctca gcatagccga cgtcatccgt cttattgagt gcgacaacca
     7861 tctgcccgac ccccagagtt cgtgcaagaa acgcatgttc tctggcttgc ccaccgggtt
     7921 cagtagccgc ttctgtttca cctggcttga gcgagacaac tagtatggct acgtctgcct
     7981 ctgatgcgcc tgttatcata ttcttaataa agtctcggtg acctggagca tcaatcaaag
     8041 tgtaaaaata cttttgcgtt tcaaacttct gaaaagcgag atctatggta atacctctgt
     8101 ccctttcgtc cttaatactg tctagaaccc aagcatactt gaaggtatca ccttttcctg
     8161 ttgcttctga ctccttagca aatgcatcaa tggtgcgttg ttcgataaca cccaaatcta
     8221 ccatcatatg gcctacgata gttgatttac cgttatcgac atgacctaca accactaaat
     8281 tcatgtgtgg ttttttgctt gcgctcatat cgggtaaaca ttcaaagggg ctttatttgt
     8341 atttcgataa aattgtttcc taaatattgt aacaattata atgatatgat gtcgatgttg
     8401 atgaaactgc tttttgatga gctctataaa caaggcaata gcaagagatc cagcggaatg
     8461 tattcgcaga aaaggacaga cagacccgca ggtttcaaat cagactgtta tgtactaaaa
     8521 taagagaaaa caatgctctc atatctattt atctctctat tgcactcgtc atggaactga
     8581 tggcaattaa gcgccaattg accggtttga tattgtccgc cgataatcgt cttcaagtct
     8641 tacaatgtca tcttcattaa atctgccata tgatatttca agaataatgc attgtccttc
     8701 tactgctttc accctatgtt ttgcacccag tggaatcaca atactttcac cttctttcaa
     8761 aattattaat ttcttctcta gttgcacctc tgcaataccc tttacgattt tccagaattc
     8821 ccatctttcg ctgtgatact gaagactaag tctggagttc gcattaacat agattagttt
     8881 gactgtgcat tgctgatttt cgtgaaattt ttcaaagctt ccccaaggtc tatcctcgga
     8941 gtaaatgtcc atgccaagaa aacgtacaaa agtaatttaa taattactgt tctatattat
     9001 tcaaacatct actagtttct ttcaatatca ccgtttcttt tttgaattta ttttttttta
     9061 tttggacatg catttagctg agtagaaaat atgctacaca cagatcataa gtaacttggt
     9121 ataaaccagt tgtcgtttca ttgcacaaaa gaatgaatga ataaatgagt gggtgaatac
     9181 gaattttata gttcatagtt catagttcat a
//
LOCUS       DAOR01000063            2603 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_22205, whole genome shotgun sequence.
ACCESSION   DAOR01000063 DAOR01000000
VERSION     DAOR01000063.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_22205"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          798 a          501 c          449 g          855 t
ORIGIN      
        1 gtcttataat tacgccacaa atggtaacaa aggccacaat catccaatag aattagaaaa
       61 gttaatgaac tcttatggat tatcgttcgt tacttcactt ttaaaaatat ctgtaaaagc
      121 aaatggctct aagacactgg aacaaatcgt aaacaccgct aaacttattg ttcaaaactt
      181 tctcaagatc acaagcctct ctcaggttac gtggcatgat tgggtttttc ttgtcgttta
      241 tgataaggtt gagaatattc caaatactgc tcagccaata ttttctgaat tcgattcacc
      301 aaaagttaag gttcctgcat tactccgagt agctcccctt gcatattcaa atgatttcat
      361 taatgcagcg tggaaaggct attccgataa acttgaaaag atgtatgctt ttgattctgc
      421 attagaatgg cttgtagaat caaacgtgac aacattgttt gaaactcgat ttatcaatgc
      481 aacaacatgc ttagagatgt tgatggatag gtttcattct caaaataata cagaattcct
      541 tcttgatgaa aagacattca aagaattttt ttgtgcaatc aggaagactg caagtgagtt
      601 tctcaaatcg aggggggttg gtcaggaaat tcgtaacgca ctttacaatt cattgcgagg
      661 aagccgaaga cgctcgtata aagacaaagc aataatctta cttgatcatt ggggtatcac
      721 ttactctgac acgggaatca ctatagatga tataaaggat atacgtaacg ccataaccca
      781 tacaggaaga tatcacgcaa gcaaccaaga ggaattctca aatgcaataa gagtatacca
      841 tgggataatg acgattctta tacgaatatt tcttgccatg ttagattatc atgagtatta
      901 tcatgatcca tggcaggata gatggattag attcagggac ctgtgtagta agattgcttc
      961 tccatgattg ctatttatcg tctatactta ttattacaat cacggaattg cagtacgaca
     1021 ctatcattag aggctacgac tttctgaaaa taacactatc aatcttcatt agtttattcg
     1081 tattttccat aaccgagccc tttcctaaga aaaacacctc gtcctacaaa aagatcatac
     1141 tttataaccc atactcttga taactcaaac ttattgaccg gccgccttat gaacttctgg
     1201 tttgccaggt tcttggggtt cgtgggttat tttagaaata caactataga atttattaac
     1261 ttgcttgact agatcaggat taccggtgtt tactgcctct tggattactg gcccagaact
     1321 cacgctgcta acgagactca tgccattgtt ttacctgaaa atgaggtgta gaagattctt
     1381 gaaattagca cgggtttgga ataggtacga ggaggttcac cataatttcg atcaggataa
     1441 ccaacatttg attcaactta gaacaaacct aaggtgagaa ctgctgcaaa ctttagttag
     1501 tagacactta aatcgagtaa tgtctaatga gctgctatat taataaatat cacaatatat
     1561 tttcacaaac tataatgtcc tgtcctacta acccaaacta tgactaaggt ttgtatttct
     1621 agcgtttaat gctgctcaaa taccactatt tagatgctgt aattgctatt cttcaatcat
     1681 aattatgcat ttttcagcct tcagcatttt gctgatcagt acaagatatc tttaagacta
     1741 gaaatttttt aatgttacct aagttatttt ttgattaatc aggacaaaaa gaatcattaa
     1801 aaatcggccg cattcttatt ttattattat tatctcgatt cttcccttgt acacaaattc
     1861 atcttaccag ccattataat ggtatccaat ttcctttcat agatacacta aagcctccgt
     1921 tacccttaag gttatgaatt acacgatgtc taactaaagt accaatttaa gatgaggatc
     1981 tatacaactg aagaaatatg gcatactcta actcaaatca agatgatttc ttagatcttt
     2041 agatatgttt ctgatggtca cttctgttac tcctgctgtt tgtgcgaaaa cagtctggcc
     2101 tatattatta catccgttaa tcaaacatga tatgtaaaga attgatgcag caagccccat
     2161 agggcttttt ccagcagata gctcactttt agtggcagca ttcatgatat tgattgcttt
     2221 ctgctttatc ttctggctca ggttaatctg gttcgctatc ctagcaatgc actttattgg
     2281 atcgacagta ggaattctga tgtcaagttc aattaccata tctctatact gccgtgaaat
     2341 tgctttgcgt ttaaggttgc taattagaca aatctcattg agtgttctag gagtcccttc
     2401 ttctctgcat gctagataga gagcagcagc taatgctgct gatgtctccc ttccacgtcc
     2461 taatcctctt tcacgggcct ttctatatat gtacgcagtt ttctcaatta ttgcatctgg
     2521 aagcccaagt ttatccttca atatgttaag ttgattaaag gcagttccga ggtttctact
     2581 agcgctagct tgtgttctaa gat
//
LOCUS       DAOR01000064            5060 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_22204, whole genome shotgun sequence.
ACCESSION   DAOR01000064 DAOR01000000
VERSION     DAOR01000064.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_22204"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1552 a          979 c          921 g         1608 t
ORIGIN      
        1 tgcttttgta ggatctccaa agaatgtatc tggagaggat tggacttggt tggttataac
       61 gacagcgatg ttgaatatct ctgccaatct aatgagttta tgtagcatac cattgagttt
      121 ttgttgtcga ttggccaaag tacctcttcc caaaaattct gctctatgta aggatattat
      181 actgtcaata attactaatt tggctttaaa ctcattgacg tatctaccta ggtctttgac
      241 tattagttcg agatgtgaac tattgtagac cttgcatact gcaacgcttt ttaatacatg
      301 tctgtgatca agtcctctgg ctctcgctat ctgctccact ctttcaggtc taaaagttcc
      361 ttccgtgtct atgtaaatta caccactatc cattccacct gaatcaatag gttgtctagc
      421 agtagcacac aatgtatgac atatctgtga tttcccagat ccaaattctc cataaaattc
      481 agttactgcc tgtgtttcta ttcctccgaa aaacagctcg tcaagagctc tagaccctgt
      541 ggaacatcgg agcattgctt ttcttttttc tagggctgca tcggcagtaa gaaactcctt
      601 atctatcacc ttagaatctc tcaagagctt ttgggctcct atcacaaatg tagcagcact
      661 ttcctttgaa gtattgatat ctattgcgag ctcctctgaa ctggctactg caagatccat
      721 cacagaaaca atgcctgcat ctttaagctt tttagccgtc gttggaccga tcccttcaat
      781 atcgtgtatt tccagatcta ccatgggtgg taatactgtt gatggagttg tagtgtcgta
      841 actaggtagg gttacttcat tatccacatc actattggat tcgttttctg cagaagctct
      901 tctatctatc acaaaatgtt cgcaaattaa taatagtaat tccttgtatt agaaggtagg
      961 agtccgtgtt ggggagggag agtatcatag aaaggtgttc gtcgtctgcc gtgactgtgt
     1021 ctcagtaatt ttgcaaccgt aacgattcag acgaaccaag ttgttgcaaa tgtaaagacc
     1081 aatatcgata cagaaccatg acgtgagttt ttagttatac aatttccatt tgtcccgagt
     1141 gggatctaat aaagcattag taatacagct tttaatggcg tcttccagca aacttacaga
     1201 tgaaatgaaa ataggtgagt taattcatag gtaaataaat atcaaaaata acctaactag
     1261 ccgtccttgc cgtagaaatg gttctataag atagatagta ccgttagaca aaagagaata
     1321 gattatagca atatattcag tatccttaac aattttttca atattcagtt gatggaactg
     1381 ttcatggaaa acatagatgg gacttctgta ctagaaattt cacaaatctg aatctgaatc
     1441 gatagaacgg gtttctcttt cccattcttc taccatgaag tgggtgttat gtttaatcat
     1501 ctgttttgat atccaatcta acatttaaac aatttctgca acgccatact atgaggctgc
     1561 aaaatattac atatgggcac cttcgaaata actaattcca ttgccttgca aaaatccatt
     1621 ttgaagtaac gcactaacac acctactgct atagcgttgc ttctggacgc accaacacga
     1681 caacatataa ccaccttctc gcctagttcc aaataactcg taactctgac aatatttagg
     1741 agatactcag acggaacatt ctctccatct atcaatgttc ttacatctat aatcctaaaa
     1801 tcatcaataa agcctatctc gcagttcaca tatctttcga taattccagg attcccgatt
     1861 ccgtgagttg caagattagt taaaatattg aacatagtaa ttatgttata ctcacaacgt
     1921 ctgtcttaaa agtatctcac atagtcaact atgtttggat ttctggattt ctcataaatg
     1981 gctggtctac atggaaaatg gaatacaata ggtacctgcg ataatatcat ataaaattga
     2041 taccagattg ggctacaccg agcaagtgaa gattttgaga attctagcag ctagtgtgtt
     2101 gggtttcaaa agcgcaccgg agattaaaat tcatctaatt taataagtca agatgacgtt
     2161 ttagatcctt tagtctcgtt cttagtgtca tgtcagttat atctgctgca ttagcaattt
     2221 ctttttgagt tttgatctca cctgtcctta tgcaagatgc gtaaattacc gttgcggcaa
     2281 gtcccattgg atctttacca gctgaaatct cgatttcagt aacaatattc ataaggttga
     2341 ttgcttgatg ttttgttttt tcagttattt cggctttatt cgcaattcta gtaatacatt
     2401 ttataggatc gaccatgggt atcttaaggt caagtcctag cagtaactgt ctatagcatt
     2461 tggcaacaga ttttcttttg acattactct ttgttgctat atcgtttaac gttctcggta
     2521 tgcccatttc tctgcaggca gtatatattg cagcaactaa tactgcagat attgaccttc
     2581 ctagtaccag tctattttcc tgagcctttc tgtaaatata agcggtcttc tcaattactg
     2641 cactgggcaa cccaagttta tctttaagtg tataaagttg agcgaatgcc tgagtcagat
     2701 ttttgtctgt ggaagtatat acttgggttc tagaatccca cgttctcaat ctttctataa
     2761 cagaaagcat agatgaatta attttactcc cttttgcatc tttattggtt cttcctataa
     2821 tagtagatag acccatatca gagcgagcta gcgaaaaagg cattcccgtt ctactttcgt
     2881 cgtttccttc acctcccact gccactgtgg caaaagtacg ccattccgga cctttttcct
     2941 ctgttttatc cgatattact actccgcaat gctcacaaac aatttcgccc gagtcaggaa
     3001 tagtaactaa ctgttcagaa ccacaaacag agcattcatc agttgataat gctttcatct
     3061 ttttttagtt agacacatca tatagtgatc acgacaataa atttgtttgt tgttatgatc
     3121 tcacaacggc cgatcacctc tatcagggat cttttgaaag gcaagcattc cctagttttt
     3181 atcaactttg catatagctg agtaggaagt cgtttaaaat cctctaatga cgcagaatgt
     3241 gattccaact attgtcatcg tcatgatgat atccgtattg cgctaggagt gttgccagaa
     3301 aacaatatgc ttcataaagc aaagctcgaa cctgatcgca tcttttttat gccgtactgt
     3361 cttactactc tttcaaatag taacacggac attacgaata aagttacgcc agcgaaatga
     3421 cccgctgtgc tcggcacaaa atacaagtaa aaaatgatag tgtttcttca gatatgaatg
     3481 acattaagct ttttctaaat ttgttcgata cacaggatta ggagtagatt aataagtgta
     3541 atcaatgcat acgtctccaa aaatcataac ttatcaaccc taatcctatg actataagta
     3601 ttatcggtat taatgaatag acactggttc caagtattcc tcttgcataa ttaatagcat
     3661 ccaaaatcag agaggcaaac aaagcaataa caccagccaa aaagagttta gaaaagtaaa
     3721 atcttgccaa tagttatcag agattgtatt aaagtcgcta tgctattaat attaagaatt
     3781 cactggggct attgcattgt tccttcctaa aatctcgtac aaccatagga gtgaaattca
     3841 atctgattct gaattcaaag ctacattatt cagccagttc ccttgagcca atgtccagat
     3901 attgttgaaa tgtggttata tttttctgtc attaatggtg atgcaagtac acttaaatca
     3961 aatgtttccc actgaaaact agaaattact gaataacgcg gtacatgttc aggatgataa
     4021 cgatagatgc taattggctt gcatctcatt tggattgtcc cgacatagtc attatagatg
     4081 caagaggaat tataccttac aggtttagac atatccagaa tgctaggcca cttagtctag
     4141 aaggcgttat tttaattgcc gataatggag ctaatcttgt catagatccg cctacagctg
     4201 aaaaactctt cagctttctt ggcattgatg aatctaagaa agttgtagtt tacggagaga
     4261 gtattgatcc gtcagcaggt aggatagtct ggactttgat gtatcatgga catccagatg
     4321 taaaactttt agatatcagt tttagccaat ggcagaaagc cggatttcca atcacaagag
     4381 aattgattcc agcacaaaga caagaagaaa aagcagcagc tccaattggg agtggtgtcg
     4441 gtcctcctca tcgattcaag tcgaaaataa atccaacaat cagagcagaa gctgattata
     4501 taaagacaaa acaagttgat cctaatgtag taattgttga tgccagaacc cctcaggaac
     4561 attttcaggc tagaattcca gggtcaatat tagataattg ggaagaaggg ataggtcaaa
     4621 atggcgaaat gattaagagt atagcagaat tagaaaagga ttttgaagaa aaaggtatta
     4681 ccagagacaa ggaaatcatc tgttattgtc atgcaggaat aagggcttct cacaaatatc
     4741 tacaattaaa gcaagctggt tatgatagag ttaaggtata tgatggctca ataattgatt
     4801 gggcgcagcg tcataatctg ataagataac gtaatcgcaa atttgatgtt gatggataaa
     4861 cggtaaaaag ggtttactat ttatcgataa taatatggca ccgagactaa ctcagatcaa
     4921 gatgatttct aagattctta catatgttcc taatggtcac ttctgttact cctgctgttt
     4981 gtgcgaaaac agtctggccc atattattac atccattaat cagacacgat aggtagagag
     5041 ttgacgcagc aagccccata
//
LOCUS       DAOR01000065           31330 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_9317, whole genome shotgun sequence.
ACCESSION   DAOR01000065 DAOR01000000
VERSION     DAOR01000065.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 31330)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 31330)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31330
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_9317"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         9630 a         6111 c         6058 g         9531 t
ORIGIN      
        1 aaagcataaa ttctgttagc gccattcatt caatcataag ttgagtatta caacagacac
       61 taaaaaacat gtgaagaata aaactaagat cacttttaac gacagagaag ttggttacgt
      121 tatggttgag tttatagaat ggcatgtcga cgatatatgt gacgactgtc ttgaagaaga
      181 cgaatgcact caatgccata gagatttggt tgacattaac acttatgatt ttatacatag
      241 actgattgca aatacaaaag aattcccaga ctggaagggc gttgcagaaa tatattttac
      301 gactgattcg acttggttgt gttacggtgg aaaagatgct gatggcaaca tactaattac
      361 aagaatgatc ccgattcata gcatagacaa tatccacata gattcaactg agccaaagtt
      421 tagcgaaagc aaaatcaatc acttagatac ttccgaaaaa aagagtttga aatgacatat
      481 atgtcaacaa gacaattaca gcaaaaagaa catatgaaga agctactgtt agagtttgca
      541 acaaaactcg acattcacga taatcatgta attgaaaagg ctgataatat tttcaaacaa
      601 gcatttacaa atggacttgt aaaagggcga tctgtttcaa caatgatatg cgctgcgata
      661 tacgctgcat gccacttgtc taatatacca atttctttag gcgacgtttg tagcgcatgt
      721 aatactaaac gaaaagattt aggtaggtgt taccgagagc ttgttgtaga gcttgactta
      781 aaaataactc aaagtgattc aggagagtat atagataaag tcataaattc attacgctca
      841 aacatgacaa atacaaaatt caacctaatt caacaaacta aaacatcagc cttagtactt
      901 gtaaataaag ccaaacataa taatcttctt gatggtagag atcctatcag ttcagctgca
      961 accgctttat acatagcatg tatgatgagt agcatagata tttcacaagc tgaaattgct
     1021 acggctgtag gaatagcccc cgttactatt cgtaatcatt ataaggtttt caagcagctt
     1081 cttaagaaat gagcgggcga cgcttgtatg cgctggtcat attatttgtc atccagcaaa
     1141 gaattcatta gctgcgtctt ctatttcatc aagacttctg tcgggctctg agcttgaatt
     1201 attctcaaga tatatagaga atcgtctaat tataacaagc ctcgctattg cttcttcatc
     1261 gctactgaac aagaaatgct tcttaatttc atatcccata tttctcaact tacctagaaa
     1321 ataatttgtt ttactgtttg gatcgttcaa agcagcaatt gtagcttctg aacaagtaat
     1381 tagatcgggc ttagttgctc gtattagtcg cagcagtctt tctaaacttc caaactcata
     1441 cggcttatag tctggtccca ttctaggaat atagatttca ttctgtaaat ctcccaggta
     1501 atcaaaatct acatcaataa ctgtgtcgtt atttactaaa gttgcaaatg aaacaatatc
     1561 ttttggtggg cttaaggcaa gactgatatc tagttgcttt agccaatagg attccgcttt
     1621 aacttctcct tcgatacaaa ctcttggtgt cacaatcttt atctctgtat taggaaacca
     1681 ctttctagcc aacatatgta tcgctgaatc ggttaacgca aagtgcaaac ctttatcgta
     1741 tccgttataa ccacgaacag cattccaaac tgtttccagt gctccaacaa tatctacgtc
     1801 taaatgcgaa tcaaacgaca gaatattctc aggttcataa tcttctttca taagggcgtc
     1861 tataacgctg gaatggaacg agcttaacaa cacccttttc aaatcagata tccccatttt
     1921 gatacgaaat attcaaagat ttttccattt ccacaataca tgaatcacat aaaaatacaa
     1981 gtgtgctatc ctgcgttaca ggattaacca tagccaagtt gccacaatcg cataatattg
     2041 cctcttcgtt gtgagttata gcgctctcag tcatatcaat aacttgtctt gttacgatat
     2101 ctgttttgtc tatgttatta ttgtcactta ccttatctcc agttgctggt atagcctcag
     2161 taaatatttt tccaccaggc ggcaaaccat atggcttaac atatgcagat catattaaaa
     2221 acttctggaa atggtcattg gggataccag ccaaagttaa tccctctaat gatcctacag
     2281 gagcgaaatg tgcgactggt caattgaata caacttcgtc agttttttac ctggctttta
     2341 atactggagg cttatctcag agaacatgta aagtcccagc tgggaaaggt ctgtttatac
     2401 cagtaatgca agcagagatt tctgataagg agagtccaaa ggcatcaaca caggatttga
     2461 actcaaccgt aacgactgat caaaatagtg ttaacagtct gtatctcaaa ataggagata
     2521 aagagtacaa ctatcaggat cttcttaaat acagaactaa tacgggtatt tttgatgttg
     2581 tctaccctaa taacggaatt tttggagtta tgcaaggtgg cccttctaaa gatatagctg
     2641 atggatttta tataataact gaacctctgg ccaaaggcac ttacccaatc catttcaaat
     2701 caagtttact ttgcacaggt acaggttgtg tagacaccaa ttttgctcag gatatcacat
     2761 ataccgtaat tgtacaatag ataatcattg ttccttgatg ttatcttagg aaaattaaga
     2821 ttgagattca gaaactgctc aatgaaatat acgaataagt gaaaattagt gaaaaattta
     2881 tgagaagaag acgtggcata gactccctga ctgcagcgtc tgatatgtat ggaagcctaa
     2941 caatatatta tgcaaaccgt taaactattt catgcagtct gaatctaacg ctgcattaga
     3001 caatgcaaga catgaggttg aattttgtta caaacagctt actgaattgg aaccgtatgc
     3061 aaaagctcta tcggacaata agcctagaga aattacaaaa gacaatttga ttcaaaacgc
     3121 actttctacc atagagtcct ccaattttga taaacagttc agagatgttc tgattaaaac
     3181 tatgatggca caagtgaaat acataaagct tttagaagaa aggattggca aatgaaactg
     3241 aatggtcttg ttttaaacac cactatcctg tatacttgac caaagaatgt ctaagcaggt
     3301 taattgactt ttacaaggca aagaaatgac ttgtaaaggt atctgcatca atcatgaagc
     3361 tgttaaacga cttcgtgggt atcgttatat tgaaggacaa aaacgttgtc agatatgctg
     3421 tgtgtttctg atatgggatg gtgctttttg tccttgttgt ggtatgagat tgaggactag
     3481 gccacgttct agctactata aagccaagtg gcatgaaatg atatctatac ctatttagac
     3541 atgttaatac agtttatcca actttaattt tttgcctatt aaatgaaaca caggctcgac
     3601 ctcctaggac actcctgtca tctgttcttt ttttgggcca acatggacta acgataacat
     3661 agaaaagagc aattcttcat tatttctttg aataaaacga caaaacgagc tgtcgtttat
     3721 atataatgct gaatgcattt tatatattcc aatttgctga aggatttggc taaaaacagg
     3781 ctagactcac aaaaaccaag aaaagaggga cttacctata tcatagatag gtttcagggc
     3841 cttgacaaag agaactttga gatactcgca ccattcatag atatagtcaa gattcatggc
     3901 gcgctcccgt tgcttgcctc cgatacgcta ttgaaacaga aaattaaatt ctatcatgat
     3961 ctaggtgtac aagtatcaac aggaagtaca attaccgaat tcgcaatttt ggaacactca
     4021 ttggaaagac ttgcgaaaga ggcagccgct attggatttg acacgataga gattggtgaa
     4081 aatagtgtag acataagtat ggaacgtaag agcaagatta tggatatggt aacttccctc
     4141 gatatcaatg ttcaatggaa ggtaggcagg aaagatccta ggcatcagct gaatgccgaa
     4201 gaaacgtcat ataaaatcgg tgatgcctta aagttaggat ccaaaaaggt ggtgctcgag
     4261 gcaaacgaag gagtcagcgt aggaatttat gatgaaaaaa gtctaataaa atggaacctc
     4321 atcggggcaa taacagcaaa atttccaccc agcactttca tattcgaggc tcctctggag
     4381 tcacaacaat cagctctgat tgcagagttt ggacagcggg taaatttagc tgaaattcat
     4441 ccagggtccg tcgcatcagt cgagtctcaa cgacgaggat ttctttcgaa agcagctttt
     4501 ggtgtatcat atttgcgtaa ggatccggag ggtggccctg ctgcaaaatt catttattat
     4561 attattaaga caaagcaccc tattgaacag agtgatttga taagtctgag ccacctgcct
     4621 agaagaactg tccaaggagc ggttgatgag ctgaaactac aaggcttaat aattggaaga
     4681 aatagtcttg acgatgcaag gaagagagtt tatgtcccaa ttcattcaga ctggctctga
     4741 atgccgtttg actctaaatt tgggtcataa agtctgccat cgaaaacacg ttcgcttgac
     4801 gacaagttgg ttttataata aaacactaga tgtaaaattt aagagattaa gtaacccgaa
     4861 tcttcttatt atactgttag tcacatttct tataaatcat aagtttatac cacaattcaa
     4921 tgagtgcaaa aatgtctgtg ttcctagaag tcagtgctat cgtgacggca ttatcgctga
     4981 tagtagctag tgctttcgtt actccagctt tcgcattgaa gcggtacttt aattgcgtga
     5041 ctgaaattgc caataaatcg gggaaactca ctatagctga tgtcaacagt tgttatgata
     5101 aagagtttca cggtagcagc catgtaagca acagtgcatc aacttccaat gcaaacggta
     5161 aatgattaga ttggaaaaac gggatggatt taagaatact gctagaatta tagtagtggg
     5221 ggcagtctaa cactccctca ctgatatttc aattgtcacg atttgattta tttattattt
     5281 tattctattc ttagaatagt aagcctagtg ggaagtcttt gtattagtga tataaccact
     5341 acgatactta attaatctaa atcattccta cacttagcca gattcgccac catatgtgcc
     5401 tcacatactt attttttgtg atattatgaa ttgattttgt cttatttttt cgtggacgct
     5461 gctgaattta ctaattgatt attagagaac acattattct ttgatgttgc atcttgaaca
     5521 atcttcagtc cttgtggtgt agcgctaact atcttgttag acgagaatgt attttctgaa
     5581 gaacccttgt tgacaagtaa agcatctgag attgagttca ttattgtatt attgtatatt
     5641 ttgtttcttg ttgacccata tccaacatta attccatctc cactatttga aattgtgttg
     5701 ttatatatca tattgttatg tgattgtgaa acaaggatgc cttttggttc attatagata
     5761 atattgttcc tggcaataga attatacata tttctactaa agtctaatcc ctcacccgcg
     5821 ttattatgaa ctttattatt ttcaattaag acattgtagc aattaagtga gcagattata
     5881 ccagttccgt tattatcata tactttatta tttctaatta tcatatcgtg tgtccctgta
     5941 tgtgggtcaa gaccataatg gccactatta cggataatat tgttctcaat aatcatatga
     6001 cctacgccaa aagtatatag tccaaagtac acatggtgta tatcgttatt tcttatgata
     6061 cttccatcgc ctccgtaata atagctcagc cctgaaccgc ctttatgctg accttgttca
     6121 tagcctaaat atgcaatctc tgaatttgtg atatctgttg tgcctgttgc attattttca
     6181 actacgatag atggcctagg agcaccggga tgcgtgatgt cgccagtcct tgatccatta
     6241 gttatagcat agtaatttgt tgttggatcc caagatgtta tctttactga atcaatcttt
     6301 agactaccat gtacatctaa gatgtatgca ggagctatct tagcagaacc agcaccacgt
     6361 gttacctttg agcttatctt tagccactta gtatttgtag aatcaatatg gaatgtagct
     6421 cctttagcaa ttattagatt tgcatctaga aaccatatat tactactacc accttgtggt
     6481 gaagattgct tgctcaatac gttattatct tgcagtttat tgtaaatttc tgtcatgctt
     6541 acagaagtac agctgacggt aattgttcta gttgatggag tgtaagtaat acacgatgga
     6601 gaagacgaac ctgtattgcc ctcttttatc ggtgaagaag gagaaagagt ggaagaaaag
     6661 acagtttggt gtttgctgat acctgccttc tccgtagtga acagaggctt actctcagcg
     6721 gtattagtcg agaatataat tcctattgct actaatatgc agatgataac aattaagggc
     6781 gttaaggtca tccggacatg atgtttcaat tctcactcaa aattttcaaa aagtggaata
     6841 tatacttttg actttaggtc atcctcgagc tgaattgcag ggccggctcg tggtagcgcc
     6901 tactgcttag ctacatgtga cggaatgttg ctccatgatg agctgaacga tcagaggggc
     6961 tgactccctt gtttgaatag tctatattgt tattatacat aaaatcattc gctgaaaacc
     7021 gagcaaaccc tgttttatgg gactcggtta ctataacaaa tggttttatt caagcaccct
     7081 tcaagtaaaa agtattgctc tgtgtattgt atcccagctg cttgaaagtt aacaccctaa
     7141 aaccattatc atgaagatat ttcattaact gatcaaacag acctgagtta gtatgatatg
     7201 gcatattggt agtcaagtcg acgttatgaa acgtaactaa aggaactgca tttatctttc
     7261 ctccttgatt gtacttggcc tggctattga ctatctttac aaagtcagaa aatattgcag
     7321 cgttattaaa cgagtcctta atttctacta catcgaaaga taaacttcta gcatcatact
     7381 tgtttgcgta attcagttta ccagcaggag agtatgtcct gcaatcagtt tgtgggttct
     7441 ttttgaatcc aacgcaatct aagaacatta gaggttctgt cccagatctg gccatattat
     7501 aatattttgc aactgtattt actacgacgg ggttatttga tccttcattg tatggataag
     7561 caaagattgt agaattatat ccatggctag caaggcattg ctttgagccg ccaatttcaa
     7621 aatccagctg actttgagac agggtgttaa gatgagcatg agtcatagta tgtgattcta
     7681 tatccatacc atcgctctgc atggcagcta tgtcttgcca attcatagct cctgtgtcag
     7741 ttgtcttgcc acaaatgaca aaaaaggtag ccttaaatcc atacttgtct aaaatgggtt
     7801 ttgcataagt gaaatctcct tttctgttat catcaaaaga taaaattact actttattac
     7861 taggagtact gctacttgta atgcctgcat tgatgtaagc tccgtattga tttggaggag
     7921 gtaattgatt agtgagatat tgaagctctc cgtaagctat gattggtaac aatgctgtca
     7981 cagttattat tacaaccgcg gtagcgacca gatcttgaaa aatccttctc ctgcttaaca
     8041 ataaaattat caagaattat ggtcaatacc cacgtataat cattgtccaa cgaagattcg
     8101 caaaatgatt cagtttatat cgtgattcct ttactatttt gtgtatctca atcaacaatt
     8161 ttgtcataga caagacttag caagaagatt cgaagaaatt atctggatat tctgggtcag
     8221 ccacacctgt ccttctcgtg actgcatgtg atacgtatgc tatataccct ggttaattta
     8281 aaattgctct acttatgcta taagcctttc atttccgatt ggtttatgaa taatccaatt
     8341 atcatcaaac ctatctaatg cttcttttat aaaaacttcg tgatggacgt cgccggctgt
     8401 caaaattaga tttatcttct gcaattctaa tttcctgata taggtaaaga ctgttcaata
     8461 ttgacatcat aatatctata attaataggt caaacaaatc tgctttcaaa ttttgtaacg
     8521 ccgctagagg ctcagcaaat gagtctacta cgactagatt aatatcatcg tcatcatcta
     8581 ctactaaaat tcgttttagc gtaggattct ttattcgacc acgtagttgc tgcttgcgat
     8641 aaccctgact tgtacttatc tgaagatgac tgggagtgac cctttcatgt aggtgattgc
     8701 gggctgtgag gaagtgggct gacgtggttt agtaagaaac ctcacttgag gtatcctcct
     8761 tcttcaaaca taattcaata tctcaaatgc atttactttg cttcctttaa tgcatgtttt
     8821 tgattcgtaa aaaaacgccg cttatccacg aaaatcggca gaatgactga actgccaaga
     8881 aagtttgata ctcttctttt ctgcatcacg ggcatcatga catccttgta gttgaatcgc
     8941 taatgaaaat agacttgcca tatatctcga cagtcagctt gtttattatc ttcacatgga
     9001 ataatgtttt ctcccggggt tgaaaatcct atttccccct cagcagtaac aaccccagat
     9061 tgttattcaa tggtcgatgt tcagtgtctg tctcttatta tcgatatttc ttaacttcct
     9121 atcactttta aatctgatat attataggta tcgccctgtg tatttccctg gaaagtcagc
     9181 ttgacaaatc ttgctgtaac cggtgagtca gaaaagctaa attgttctct ccctgaattc
     9241 atgccggtat tttgagcaga accatggcta acgaagtgta ctccatctgt tgatgtctgt
     9301 attgtgaaaa agtttattga cttatctccg ttggcaaatc caatttcaag attgcaaatt
     9361 gatttttctt gtcctaaatc tagctgaacc caagaacctt tcccagtagc tgacaacgtc
     9421 ggcacaagat tattattagt attattcccg ttgttgagag tatttacaag cgtgtttgaa
     9481 ttgttattgt tactgtccgt aacgcctgca atctggagtt tattttgagt accgcatgat
     9541 gcttgtgggc ttagcgattg actgccaata gaattccctt gaacgtttgc taatgtcgta
     9601 aacgatgtat attggttatt tgggaagatg gcatgtacct ctatctgcca tattcctaac
     9661 ggtcctgaaa tttgaaatgc acttgggcct actttcttga gaggatgtac tgaaggaatg
     9721 tccccgtcat agtcagtgat aaatgcatct atttttgtag gctgtttgct gaattgaaag
     9781 cttacggtat tgcctggttg cactgtcaag ttagcattag tatctcctgg ctgagcagga
     9841 aataggactt tatttagctg cccgctgtcg gtaaaaatga agggggacat accatattta
     9901 tgattattaa acaccaattt ggcttgggga gctatcgaca cgggagtcga tatcggtcca
     9961 gctgaagtac tagcggcata tgctagtttg aacggtgaga aagtgacggg cgtcgaaata
    10021 accagaatag ttacgactac caagaatgtt gtgacaagaa cattttgtgt accttcaatc
    10081 cctttcaaac ggcgatctcc catcctttgt tcataggcaa taattgtatt ggaggtattt
    10141 acaccatatt gaactaacta ccaaaataat aatcagtatg aaagatagcg acgttattaa
    10201 tatttcagct gagactcgat gtgcattgga tgaacacagg gtactacgac agtagtagta
    10261 tttagttata taagtattcg acaatctatt attctcgata acattgttgt atgaaaacta
    10321 ttttaattaa acctctaatc caatgtaatt agaaaagaaa tgaagacaca agtacaagaa
    10381 cctattatca agcgttggaa tagaggaata aaaatagtta gagctggaag aggttattca
    10441 aaggctgagt tggaggaagt tggtctgcgt gatgtcagga gcgcaagaaa tcatggaata
    10501 ccggtggatg cattgagaag tactaactat cctgagaacg ttgagcaatt aagagctgta
    10561 gcaaagacaa ttatcgactc cagaaaacca aaaactgaaa gacgggatag tgaaaagaga
    10621 actaagatca aaagaaagac acccaccacc aagaataaaa aatcgacaag gaaaaaagct
    10681 tcggaatgag gtgatcagct tcgtttacaa gaatggacat caaaaactaa gttgatgaac
    10741 aattaaaaaa tggcaaagtg atcaaggaat tagggattga tagtgtttgg aatagtggaa
    10801 atcgcacttc gccacattat tataatgcta gaatattacc attaggatca tgcgtcttag
    10861 gtattggaga ataggtattc ctgatctcgc cgaggcaaat tatcctattg aaaaagtcaa
    10921 tcattgggaa ataaaatgca tgcagggaga gtatcagcac tttggagtgt tctggtatag
    10981 gtacggtacg ccctttgaca aagaacctgt tcatggaata tgcttctatt acaacgaaat
    11041 ccctctaccc gtagttcaag cacttacaga ttttctactg tcgaaatttg gcggtaagat
    11101 cctttacagg aagaccaggg tcttcctaca aggatcaaac gagttttcag acccaaaatc
    11161 tataggaatc ctcgcacaag aaataagtct gaaatttaat gcgcccatag agataaccat
    11221 tgaatttgaa aaggtaacaa aagaagaaca agatcaaaat actttcaact taccaccaaa
    11281 taagatgctg cctatcgtag gatcagatta atgtctaatc agaaaatcgt agaaatacac
    11341 atagatttga gatcctatct gatcagtctt agtttatgag cgtcaccttt tctgttattt
    11401 tgtttcaaat gaaaaagaat ttttaaatga gtataataag agggtactat gtctaatcca
    11461 agcctagaag aagctataag attactaact gttggtaaca ccgaggactt taatagatgg
    11521 agaatgaaga atctcaccgt aaaacctatt attagcggaa tcgactttgt aggcaaagat
    11581 ctttcgggtg cctgtcttaa tgggctctca tttgtcggta ccaatttctc tcattgcaat
    11641 ttgacacgag tcaatctagt ccaagccgac cttagcaacg ctaattttga aggagcagac
    11701 ctcactgatg gtctactatt gtatgctgaa atgaaggagt gtaatctcat cgattctaat
    11761 ctcacgagga caaattttat gtgggcagat ctgcaaagct ccgatttgtc cggatgtaaa
    11821 ttatccaaga caatattcgt caatgccaat ttgatgaatg taaaaattga taacgtcgac
    11881 caaaatggtg cgtacttaaa atactcaaaa cttaagggga cggcttggca agtagaagaa
    11941 aaagaagata tacgacacaa ggatcctaaa aaataagtaa actgttaact ggaccgacca
    12001 gacgtcgcga taaacttaaa cccttctggt tttactctca gaattgacca ttcagacggg
    12061 caagcatacc ttatgtgaga aactaacttc gaaggaagtt atagagatca cgtaacaaat
    12121 gaaagcttta attgccaagc ggcatccatc ctcgatccag cttggattaa atcgaaactg
    12181 tacgctgata catagatgct taggcttgcg atatcgcaag taattgctag ctcaggattg
    12241 atccttgctt ttaagaggct taaacaagtt cttctgcaaa ttcaggcttt tgttggctaa
    12301 tcattttccg taaccctttc atgtaatttt gggcaaacgt ggttataggt tgcgatgaat
    12361 accatatgca cccaagtacc tgacaatcgt ggcatgtccg tgctatatcc cattgcggag
    12421 cactccaaat ttcttctacg ctcttctcaa gcaggttgta agtattatcg ggactattaa
    12481 atttccagca gggaaccata actgtgccat tgccattaac aaagatgctt ttccaaacac
    12541 cacattcctc aaacattccc cgtccgtgtt ctattatttg ttcaaaatat tttgcaggaa
    12601 tcataatttg aggagtagta tgagttttca tgtatcccaa tatgctctcg cacaccttta
    12661 ccatagtttg tctatccgga agaagagaaa aattaacatc agatctgtcc tcaacgaaag
    12721 taaaggaaac tgcatttgag cccaattcct tcgctctatc aaagtaagtt tgattaatga
    12781 attcctcggt gttgtatttg gttatgacgc tattaaagta gtgggggacc ttatactcaa
    12841 ttaaaagacc gaggttgtcc attactttcc gaaatattcg tggagaaaca cctcttactt
    12901 tacagtatgg ttcttcgaac atagaatcga tgctgcaggt aataaattgg atatacctcc
    12961 ttagctcgtc aagatcaatg gtatgtagaa gagaacaatt agttatcaag gttatgggta
    13021 gctggagctt gtaaagataa cggataagtt ccataaaatc tgctcggctt gtaggttcgc
    13081 caccttcaac aattgaccag atacaatact ttgaaatttt gtcgaaaatg tccttccact
    13141 gttcagtaga aagatccttt ttcctggcaa ctttaatctg accatcctta tcagagtcgc
    13201 caaaagggca catgggacaa taaaagttac agtaatgtgt gaggctaaaa accgcaatta
    13261 atggacgtct ccttccaata atcctgttgc ccgaccactt tcccagtagc ttgatagtac
    13321 ggatagtgtc aacgaccatc tacacccatc ttgataaaag gcatttctta agcatttata
    13381 tgctgattag aagagtcgca aagactagta tatttaacga agagttaacg aagcaactct
    13441 gacgccatta gagtatgcca tgtttcttta acgtcgtgtg ttctgattag atttgcgcta
    13501 ttcaatatgc agattaattc tgctgctgct gatggaatta aacgttcttc tggttctaag
    13561 ttgaatatat ttccaatgaa tgatttcctt gaaacgcaga tacaaattgg ttttgaaaat
    13621 ctttttagtt tctttagatt tgatatcact tcaatatcac ggatgtacca tgggaggtct
    13681 gtcattcttg taaagaacgg gttttttcct tttggcctga agaagccaat agcaggatca
    13741 ataattatat tgtcagctgc aatagaggcg ctttttgcaa ttgatatact ttcagtcaga
    13801 acctttatgg ttccaaagac acttcctgag gcgagaccag atcggccgcc gctgccacca
    13861 taggctccca tgattattgg cagtccagat ctagatacaa tgtctgccat cttcttatca
    13921 tattttaacc ctgaaatatc gttgatggca tcggccccga ctttgattgc ctccttggct
    13981 acctcagctc tgggagtatc tgccgagaca ggcaaactac aactgctctt tataacatct
    14041 atagcgtccc ttattctttt gatctcctgt tcaactgaaa tgatcgtttc gagatagggt
    14101 gcagtagaca tggcaccgat atctatcata tcagcacctg tttcttgcat cttcgtggct
    14161 acattagcta tttcatttac attagtctta acggaatttt tgtaaaagga ttctgggctg
    14221 acattgatca ctcccataat cctaggctga tcctccccgc caatcctcag cttcccgata
    14281 gaagtcaata ataagttgga attacattaa ataatataat agattttata atcaataagc
    14341 gtgttattgt cttgagtgat agttatatgt tggcctccca ttcaatgctc tactctaatt
    14401 ttctggtgtt caaggagaga ccagactaga gccagtggtg aatttttaat aaatcttcga
    14461 ttggttcatt tttaagcagt tttatcaatg cgaatgcttg cggggttgac agcataggct
    14521 tatcgaaatg gtcgtctatg gctattcttg gacatgcaac ttccacaaaa acatctaggc
    14581 ccttaaagat ttgaacatgg tcattagtaa tatttgtcat tgcaattaat tggactctct
    14641 ttcctagctg ttcaaataat tttttaagtt taagtgcctg aattttggca aattgtcctt
    14701 ccttcaatcc aataataatg ccaatccctc gtgcatccat tgccttgtag attgataaaa
    14761 tggctttctt cttgaacatc tgggcgatct tgttaatttg cgaatattcc tcaaaatatg
    14821 gatctagcat gtatgtcggt ttttcggttg acattgcgac acttactgaa tgaaatctgc
    14881 tctggccaag gaaaatgtac gcatcgacta agttctggat gtcaaatgca ggatagaatt
    14941 cgcaaccaaa aacctggcca tcgttaagct gtcctttccc tttaccgatg atcactttgt
    15001 agccatactc ttcaaagatc tccttgacat taccaatttg attaaggtgt tgactgtctg
    15061 tcaataatga tagagtcttg taattttcgt tttgaagaga tgaggcacat tttcttgcaa
    15121 ctctatcaaa gctaatgtca tcataagcat tgatcattat taccctttcg ccaaatgtct
    15181 catttgcaat ggtatgacct atgttgaata aaatctcaac ccctaacatg tcggccgcct
    15241 gtgtattgag atcgcaggat ccccagcaag tatctccaat tatgtaggct ggaataccaa
    15301 acttttctgt aatattggct gcactagtct ggattctcgg aataagtccc tccggtccat
    15361 taagggcgac cgaatgcggt tttcgtgcat ctattacttc gtgtattctc ttttcgtcta
    15421 ttagcatcat tttccttttc ttcaccagtg actagtttag taaaactcaa agggtaacaa
    15481 tgccacattt taaggctttg gtgtaaggca aaatacaact atatggccgc agccgcaata
    15541 cactccacaa ataacataga ttaagagata taaccatact ggataatgca actaaagttt
    15601 gtgtacagcg agtggcgtcg tccatcgaga atcagccctt atttgcatct ctaactaact
    15661 agcgcactaa tgtactaaat gaatagtcgt gcgccaacaa gagcaaggac ggaccctaaa
    15721 gattttgtca tcttctttaa gagtgctcaa tgttaatcga ataagaattg ctaaatattt
    15781 ctggcgcctt gtcgatttgt tcgtttttac gcataataat agatattttt tcgcgatttg
    15841 gatcgacgat gattcggcac ctctgtgatt gttgcatgat gtgaaaatga tgttcaaaat
    15901 tgagaggcaa agaattatgt cttaatgatt cagggatgtg tggttcgaat tgaatcctat
    15961 tttcaagcat attcaagcgc attccaagca tcatctcttg cattgcatag acatacatcc
    16021 caacagacca agcttgaagg atgcaagagt taaagggttc aggtcggtcg cctctgtatg
    16081 tttctgcata cataccattt tcacaaagta tacgttgtgc catagattcg atgtaataaa
    16141 gtgcctgttc taacctgttg atcttaatct cactaattgc ggcccaaccc gttacaagcg
    16201 gccataccgc accatcatga taaagactcg gatgatattt tggatcgaga ctgctcagtg
    16261 ttcttactcc ccaaggggtg gttaggtcat tcttctcgat tcttgttagg acagagtgag
    16321 ctttgattcg gtctcctatc gattctgtaa gtaacatgac gagtgcattt ggtctaatac
    16381 tagaatcctt tgtaccatcc cttcttatcg tatcataata gaaatcgtcc ttgttattcc
    16441 aatattctct ctctactttg cctctgagct gatcagcaca tcgactccat cgctcttgat
    16501 tataattatc atccgcaatc atcgctagct cgcttccaat tttcaaactt tcgtagaaca
    16561 atgcctggac gtcatttgcg ctctttctcc tatcaatatg gtccatccaa gtagaatcct
    16621 gaattggtaa tttttcggcg acaccagtaa atccgttttc gactaatcca tctctatcca
    16681 catcttttaa aaatccccaa ttgacaagat caacgatctg tttccagcga ttttttagat
    16741 aatttatatc tccggtcgcc aacacatatt cacgtatcgc ccaaggaaaa agaaatgtag
    16801 aatcagctga tccgaatgta ctatcatgaa gaaatgattt accgctaatt atcatcggca
    16861 actctccttt cattgcaagc ttcgaggttt ttcttgcctg gtgatgtaga aaattgtcga
    16921 ttactgcaat aacaaaacgg taatctccga tagccaagta acctctcaac gaccaacctg
    16981 tgtctctagc ccaataatta ggaaaccttg gcagtcctgc gcaaatacct gggcctagtc
    17041 cgtcgtattc tgcctttaag tattccatac tcgtttttgc tttttcgaat gccttaacta
    17101 gttttaaaat agtagagtgc tgatgcggaa tttgactcga ggatgcaaga tttaaagtag
    17161 aacaagagat cgatttgaaa tgattttgcg tgctttccag cagttgttta taattatccc
    17221 gcatataccg gtattccttt aaacaatctt cagaactagt aaatccgccg gcggccacta
    17281 gtgcaagctc gctggtgttg cctgcttcaa tatcaagatc atatgagatc tccaaatcgt
    17341 tatcaaaaga atccattaac actctcgatg gtctcatgct cttcggcgcg actccaattg
    17401 ttccgtagaa atgcgcaaga tttttcttgg ctgcagtttg gatttgtaag caattgtctc
    17461 tcgtaagtag cttagaaaag ttactctggg aaattgcagc caagccatag ctggtaatat
    17521 tgccgtcaac tacgaagtgt atacgaatct tccgtcttga tttttccgtt ttcttcttca
    17581 aacttagttt tgaataatcc gcctctcgat ctaacaactc caatttcata acaaatcctt
    17641 tgttatcaac tggaacgaat agtgtcctct ttacttgcaa acctgccact tcgtgcctcg
    17701 tgtagagaaa tccattctca tgtctagaag ataccacgaa atttgggagt ctcttacgta
    17761 ctccatcatc catagatatt tccactagga tcctaccgaa agccctagct ggtggcacgt
    17821 acaatccccc attgtcatat cttgttccca tccaagacca gttagccgaa agcgaagcat
    17881 tcgctgaaca tacaatatat gcttgtttgc cagacagaat taacggagtt gtgaagtttt
    17941 taaaaatcgt atcctcattt gatgtaatag attcagaatc atccatcatg ttggagggaa
    18001 taaaaattct ctccttacga ttgcttaagt aatcgttaaa agtaaatggt attataatct
    18061 gttcaaatcc ttctagttta ctcaaaatga caatcttatg tttactttgt tcttccaagc
    18121 caccatcttt tattattagg aatcttgcgc tttgaaatgg cttaaatgga atggggcggt
    18181 cttccgataa tgtggctgca ttgaccacat cctctgaagt tgcgatgaaa attgattttt
    18241 ttggaactgg aatttcatcc acgtagatat ctagggatac tatattaccg cctagcggaa
    18301 caggtggatt tagcaaagag aaaataaatc catcaaattt attatctcca ttcactatcg
    18361 ttgcattttt taaactacca tcaatatatg aaagaaccgt atgtgtttta tcgagtgtcc
    18421 taaccattta gcgcacctag gagtttaatc ttctattatt gaacaaggct caacctaaat
    18481 cctaaaatat tgaaatgtaa gaacgcgtag ataaaatcat ctaaaattat caaattcgat
    18541 agatgaattt ttccttctaa gaaaggaatc acatttagtg ttctagctta aggtatttta
    18601 atattcatct gctatttaca cagcatcccg ggtctatctg ctcgaaaagc tagacgggtg
    18661 tcgtgttgat aaaaaaagga aaattccagc aggttcattg cgttgttgag agggacgtgg
    18721 gcgtcttctc gcatatggcc gatgcgttac tggtatggag gcagcctgcc agtaccacca
    18781 gaggcacaag gccccttctc aagccctaca agatccacca cgtagacgtg ttagcatggc
    18841 taccccgcct tagggttgct ccctcccgga cctcacccat ttcgacgatt cacagtcaaa
    18901 actacgcctt cgcgtggtac gctgctcata accatccgac acggcatgat ttcatttcag
    18961 caaggcaagc gggtacctca cacctctgga tttacccagc agttactgat cccagcatag
    19021 agccgatttc tgatgtgggg tacggctaac accccgatcc tccctgcctc cgtaaatatc
    19081 atagctatgg ctcattatat ttgcttaccc gaacgcaaat gattaagaag taatgtgccg
    19141 atagtggcga cgtacttgcc gagccaatga atttgattta cttgctcttt gctaggactt
    19201 gaagggtctt gcaaacagaa caaatactgc cggtagaatt ccgtccgcaa accaagcatt
    19261 ctttagagtg agataaagat cccatagacg aagtcttcaa catcttcgag atttttgtca
    19321 ttgagttgta agcgttgtac tttatccctg cgtgtcctgt ttcaagctta ttgagaaatt
    19381 cacgaagctc ggttctgatg ctttcatgca tatacggaca ttcttcggac tgaaaaggga
    19441 tgtctctgag tacagcatag aatacaattt cgtgctcata aatctctaca aatggtttta
    19501 cctttctgag accattgagt ccatattgaa ctggttctgg atacatccaa cagattctct
    19561 cagtgtctcc tgatagaagg ttaatcatga aagtttgtag ctggtcatca agattgtgtg
    19621 cagtcgcgac gacattcgca cctaccgatt ctgccatcat gtctattgct ctcctacgga
    19681 aggtgccaca catagaacag gaagtcattt tttcagacgg cctctcctgc attgcttggt
    19741 ccatgcccaa cccaaaaaga tccttatagc ttgaaatgtt cgtttccact ttgatctggg
    19801 agcaaaaatc ttttacaatt tgtaatgatt catctcggta accgtgtata ccctcgtcaa
    19861 tcgtgatagc gaccaacttt gtgcggctat gttcttcaaa aaatttcttt aacacgtata
    19921 gaagggccaa actgtctttg ccgccagaaa caccaactgc taccttatcg ctatgactaa
    19981 gcattgaaaa tttctgcata gtcttagcag ttttttgttc aatagaaagt aagaagcatt
    20041 ttttgcatag gtattcccca gagtataccc tatgatatac agttaaccca ttgttacatt
    20101 tagtgcaagt tttccttgac aggagtagca gttatgggat cgaggttatt attccttggt
    20161 cttaaaagta ggagaaaggc gaattcttac cctgtatttg gtaatcacaa ggcttttaaa
    20221 tctatagcaa aggcatttat gtctcaacct cgacttgact atgagtatgc aattcaacga
    20281 agacctgtta gatgaaatta atctcaagat aatagatata ttgagcagag attcatcaac
    20341 gccatttgtt gaaattgcca ggaagatcgg aatctctgac gcaacggtac atctgagagt
    20401 caggaggatg atagctgctc gaattatcgg caagtttaca ctttctgtaa ataatggccg
    20461 tctgggctat gaccacctcg cctttttggg tataaatgtt ggacctggtt tggctgaaga
    20521 ggttgttcgt ggattatcga atgtggaagc tgtgttagaa attcatgaaa tgcatggtag
    20581 gtttgatctt atactaaaga ttcgctctaa agatttggag gagatgagag atatagttgt
    20641 aaacaagata cgtactttac cacatattct ggaaatagag ctaatgactg ttttaaagtc
    20701 gagaaaagaa gaacaaatgg ttcctttgaa aagcgatata gcagaaaaaa cagaatctgc
    20761 aatcgcttag ccgttagaac gtgattactt actcacaatc tgctctgctg ctattgtttt
    20821 tgtttttcct tcttcgaggt ttgccaccag tctagataat catcatgcaa ttgccttcgc
    20881 ttattgacag actcattttc tgccttatcg cgtaattcat caatcttttc tcgggaagca
    20941 aatattccac aactcttgca aaggaaattt ttactattag ggtcaaattt caaatcaccg
    21001 ccacactcag gacagaagtt tgccaaatgt atcctaggtg tcaagatcct aataaaagca
    21061 ttgtgcggga agacggatta tagcattaaa gatatcataa gaaaggactc ctgctcttga
    21121 ttcaattttg catgcatgca tattatcatt atcttatcgc cggaacgttc aacctggtgc
    21181 atggacttag atcgtcattc caccatttaa ggtggtcagt tcgcccgtca tttgccatgc
    21241 accaggataa gaatttcaga tacgcaagaa attaaaatgt tcggtttgtt acacacacta
    21301 ttttactatc cgcatcaacc tttacttcca ttccagatct catagcgaaa gaagacccgg
    21361 tatcaagcgt atccacaaga ggtatatttg aaatcgcgca ccctgaggcg gtggttatgt
    21421 ctgccttgtt agtgcttatt atcgcagccg gcgccacgcc gtttactttc aacgagtata
    21481 taacatatgc ccccacacta cttccgatag ctccaggaaa aactaatact gcacctttaa
    21541 cagagctccc agacaattga tgatgtgggt cttctatgat gcccttctct gtgtctatca
    21601 tcgaaagaaa attaattcgc tcttctgtaa gaagtgtgtt tccgattcca taccccccta
    21661 caatttttct gcacttgaag cacgtgttca attgtagtca gcgcactcct tcataattgt
    21721 cttcaagtct ttcagacata ctcctactct gtttgaatga tttagataat acgcggcctt
    21781 aacactgttt gtaattaccg aatcaaagtc agcccgcgtg attaggggcg taagacatgt
    21841 gcacgagtcg cacatgaact ctccaccggc atgttcaatt cgatcgatta aaccgaggtg
    21901 tttcgcttga ttatgaattg ctcttgcaca gaaaattata caacgtttcg tcagcctttt
    21961 tccttcaatc agagctgcca ataaatgtag ctcattcaat ccaagctgag gactgcccag
    22021 agcgattaag tcaccatctt ccgctgtatt gagctcgtct ttaattgtgg ttacttcttc
    22081 cttaccacac gagataattt cttgtttcgt gtcgtcttct ttggtgaaca ttccgcaaga
    22141 gcctgaggtt ccaatcgccg ctgagagagc ttttgctttt atcgtgtcaa gtttttcttc
    22201 gcctgcatta atgccaatac aattatccct tactgtatta cccgcgaagt aacctagtag
    22261 gccataatct aattctgtag cgagttcata atcaggtttg acggtcacct ttgcatgtct
    22321 gaacttgtcc atcagaaggt tggaatacgg agatttcccc gtgattgaac ttgcaagcgc
    22381 actcagggcg ctctccctat tagttagcag tcctaaaatt gaattcgaaa atactgctgc
    22441 actgctttca gcgaagctca cagcgctacc tttttcagga atatcaaaaa tctcgtacgg
    22501 aacacatgta aacgaagtag ttgtacccaa catatggtag gacctggcta tgctcatctg
    22561 tttttgggta aaattctcgg agagatttac agtcttattt tggtcaaagc ccatcggatt
    22621 tattgtcgtt cttatcgcga ccctggcact tttactaaac tcttccaaga aatccacgcc
    22681 gctatcgccg atagtattgt agttgactcc cgatatgtgc gcccatttta cggggatcag
    22741 ccgttctgct tcggtagctt cacctattgc aagtaagatt ctatacgcaa ccgccagcga
    22801 ttcgccgcgt tcgcccctta gcatattttc ttcctccccg gtgagctcca ttccataaaa
    22861 ttgaattgtg aggtgtaata attatttttc cggcctcaag tcgaatttac cgatattaaa
    22921 tgcgactccg ctgaagacct caggagcatt cctcttttcg ctcatataaa aatcgagctt
    22981 tttccacctt caactcattg ctaccacgca tatcgcttct acaataggta aacaagcaca
    23041 caaataaacc cgtggagtta atcatataac aggtattaag accgaagatg acgatggaag
    23101 atccaaggat aaataacctg ttagacttac ttggacactc ttcgctctat cccccccagc
    23161 aacaagccgt ctcacatggt ctcctagaag gcaaaaactt gctggtcaca actcctactg
    23221 caagcggcaa gaccctaatc gcaataatgg cagcaataaa agcaattgaa aaaggaatga
    23281 aggtttttta tcttacccct ttacgggcct tagctatgga aaagtatcat gatattcgtg
    23341 tgctcgaacg tttgaatatc ctggatagaa aaatccgtat cagagttgct tccagtgatt
    23401 ataactcaac tgcacgggaa ctagaagaag cagatgttct agttttaaca aacgagaaga
    23461 tggactctct aatccgacat gaaagtagct ggatttccga tgtcggactc tttgtagtgg
    23521 atgaagttca tttgattggc gatcgggaac gcgggccgac acttgaaatg atattagcaa
    23581 tcatcagaaa agtgcatcca catgcacaga ttctggcatt aagtgcgaca gtttcaaact
    23641 ctgcagatat cgcagagtgg ttgaattgtg atctagtaga aactaactgg agaccaacga
    23701 aattgtttga gggtgtctat gaaaatggga ctatactcat gaatgatggt aaccgtgtca
    23761 aaattcacag ctattccaat gctgcaatag atgttgcctt agactccatt gataagggtg
    23821 gacaggcttt aatcttcgcc gggactcgta aacgtgcatg ttcgcttgct gctaaagctg
    23881 ccgaaggagt tcttagacga cttgatagaa ccgagaaaga gaaggctgcc acagcatcgc
    23941 tgcaaataag aaccaaaggt gaagacctcg agctcacgag taatctctcg acgcttgtat
    24001 ccaaaggtgt agctttccat catgcaggtt taggagcatc cagcagagaa attgtggaag
    24061 aagcattcaa gacaggtgta attagacttc tagttgctac tccgacgttg gcggcagggg
    24121 taaaccttcc tgcaagaaga gtggttctct caagtgttct acgatataat gccgattatg
    24181 gcgcaaatat gccaattagt gttttagaat acaaacaact gtgtggccga gcaggcagac
    24241 cgaagtacga cacatttgga gaagcaataa ttatagctga atctggagta ggttcaacag
    24301 atctttatga acactacatt cttggtgaac cagagcccgt acgctcgcag ttaataaatg
    24361 atagggcatt gagaatacat gttctcagta ttatttccac aatccctgga atgaagcagg
    24421 gcgagatata tgatttgttt gggaccacgt tgtgtgctca acattataaa agagcagtta
    24481 taactttgaa aatcgatacc gctttacgtt accttgaaga tgagaatctt atcaaatcga
    24541 aaaatgagag atacatacct actaattttg gaagactatc atcattactt tatattgacc
    24601 cgcttaccgc tgtagagttc aaaagagcaa tcgaatcagc ggtgcaatcc aatgcaaatc
    24661 caaatgatgg tgttattaat catacacttg gctttctgca tctgatcaca agttgtgctg
    24721 acttctatcc taaattttct atgagaagga aagacttaga attaactgca atcattgacg
    24781 aacatcgtaa cgaattgttc tatcaaatta gcgagtttga atgctctagg agtctattgg
    24841 cattacatga gtggatccac gaaaccagcg ataggaactt gagtgataga ctctcagttg
    24901 aacctggcga catgcataga atggtcgaga tcgctgactg gctggctcat tcgttatatg
    24961 aggttgtaaa attacttaag cgcggcgatt tgttaagaga attacatgat cttcagattc
    25021 ggataaggta cggtataaga gaagaactta ttccgcttgt ccggctaaag gagattggaa
    25081 gggcaagagc tcgagcgtta tatgatgcag gtcttgttga cgccaggaaa gttactgaaa
    25141 cacccgaggc aaaactatct gccattccaa aaatcggtcc aactttggca aaaagattaa
    25201 aagaacagat aaagaaggaa cgatattaga acggcgatgt ttgggtacga gaccgaattc
    25261 tttggcttgg tttcatccgt agttattgta attttataag ccaccatatc tacggaattg
    25321 ataacagtaa tgctatatct gtccttttgg aactatatcc tcttctatgg tacaatagtt
    25381 tctgccgctt cctttctaat catactattg ctgacgccta aagtaatcaa attgctcata
    25441 gcgaaaggaa gtgtagttca agattatcat aaacctgaaa ggcctaacat tccaagaccg
    25501 gccggcccga tcttgatcat aggaatagcg gtcgccgaga ttattcttta cttacttact
    25561 cggaacatac aggtcctcgc gattctattg actacaataa ttgcatttat tgtaggattg
    25621 atcgacgata aaaaggtcat gccagggtgg tataagcctg tagcgcttat tcctgctgcc
    25681 attccgttga ttgttttagg ggctcatgga actcatctaa atctcatatt tggggacgca
    25741 tcaattccac ttctatacat tcccctaata ctaatcataa ttccggttgc cggtaatacc
    25801 attaattcaa ttgatgtctt gaatggggtg gctagtgagg cagtgatcgt tgcaatgata
    25861 ccactcttag tttctatagc tgtcttcggc aacaacgtag tctttattgc tggacttccg
    25921 ctgttttttg gaatgattgc attttacaag tatcataagt ttccaagcaa aatatttcca
    25981 ggagattcag gcactttgtt gatcggagct atgtacggtg cgctagcaat tgctgggaga
    26041 tctgagataa taggggtcat tgccttactg ccatcagtga tgaattcatt tttgtttctt
    26101 gatagcgtca agaaaattgt tgagcataga caaattaagt ctcgacctac agtcttgatg
    26161 gaagatttta agttaatggc ctcaaaagag aagaacgcac ctacaaccct attgagatta
    26221 atcttggcag acggtcctct gtcggaaaaa gagattgcca aaaaaatact caaattggct
    26281 atatatagct ctatactagc actcgcgacc gttgttatac agtactattt tctctcaggc
    26341 tttttctcca aatgaggttc atgagtctca tactatttac tatgataata gatactgccg
    26401 gtttccgagt tctcaataaa tgtttgccac tttttatgta ttgttcttct ttagggctaa
    26461 ttcagctaac agcctaacgg tatctgtagg tacaacatca atcgcaagtg tgataataat
    26521 tatcttgata ttggtactaa ccccgtttaa agagaacgca cagtattaaa gatacgattg
    26581 tgactgcaaa ctgcaaacaa tacgtatttg gtagagtact tggtttgcta ttccttatca
    26641 gctctgtaga tctgtacaat gtttgaaaaa cctcggtttt caagaaatga ccaggttttt
    26701 ccacaaattg tacagttaat atgtcccccc ttgaaagaaa attggagttc ataatgactg
    26761 cagcgtatct gatgttcacg tttgagagcc tccaattgtc tgagagtgtc aatatgcctg
    26821 gtagagtttt cagtcactag aatagatgta cccatgagaa taagaattct ttgtggataa
    26881 tgctaacgtc tattctagta ttaatgcctc tcgtctcata atatttacaa tatgattgta
    26941 gacgcgctat cgataaataa tactcttgat tttcctcgac tctttcagcc tccaactctt
    27001 actctagaca tctcatgatt cgctaaattc tttctactac gttatcttat tacatatcga
    27061 agcataagct acgttatata gaacaatgta gactattctt ctattatggc cttagaaata
    27121 ggaggggaag catatctcaa tttcaccgaa tcattaagca ccaaaacgac attaaagcac
    27181 tatctcttct gtattgaaaa gtatgccacg tttcgcaata cctcaaatct cgacgatata
    27241 attatccaag ataaagatac gaataatgca tttagtcaag taaggaggtt tctagctaca
    27301 ttattggagc gtggcctagg tcaaggctct ataatcaact atcgccatgt attgaagcat
    27361 ttctatgaca tgaacgatat catactaaac tggaacaagc tagcaaagtt cactcgtcgg
    27421 gaggaagtga ggaagaaaga tagagcttac actagagaag agattcaaaa gatgctaggt
    27481 gtctcagatt taaagagtcg tgctattatc ttgttattcg cttcttcatt tgtaagatta
    27541 ggtggtattg ctggacttgt agttggtaac ttaatgaaaa ccaatgactt gtatcaaatt
    27601 gtagtatatg caggctcaaa tgacgaatac acgacatttt gtacacctga atgtaccaaa
    27661 gtgatagact cgtatctaga atctcgtatt agatgcggag agataataag tgatgaatcc
    27721 cctctgttcc gtcgaaagtt cgacacagat gacccgctta aggctaaaaa caacgttaag
    27781 gcagtaggca tagaaggaat ttctttcctt gtaaagcagg ccataatcaa atcaggaatc
    27841 cacgcccaag ttaaaggtca agttaaacgt aatcatggat tcaggaaatg gggagatact
    27901 acaatgataa ggaaagccat aaacgtagta gataaagaga cattgttagg gcatggaaca
    27961 ggattagatg acaagtatta tcgtcctaca gatactgaac tccttgaaga gtatcaaaaa
    28021 gcagttgata atcttactat caatgaggaa aacaggctaa aaacaaaagt ccaaaccctg
    28081 gaaaaagacc aggctagaat cggcatacta gagaagcaga tgcagacttt aatatctaca
    28141 attaatcaga tgggtgagga aggttatgtg ggtgttgatg agatagattg ggtaatagaa
    28201 caaaagaaat tggataaaat tccaaagtat gcagacctga ccaatggtgt cagaactcag
    28261 taatcatttc gttcttatcc agtttgcctt ttctaaactc tcgtagatat caggataaca
    28321 agactttaat gcagcagata actcagccac ttttccacta tccaactcat acagcgttcc
    28381 atccgccttg gggacggaca caaatgcagc aaaaaagtac tgaaacatct tcgtgatagg
    28441 aatagcatgt tgaatccagt tcataatgat tctgtcctta tctgcagtct tatctttaaa
    28501 tggacttgct gcaataataa atgcataaat cattaatgct ccaaatctag tcacaaattc
    28561 ctgtatgttt ttctctgtat ttttgagtgg aaattgagac aaatagtata agctttgtga
    28621 attgaacatg gttgcaaagt atcttaactc cttagccgcc ttatgagaga tcgaatactt
    28681 gttatccttg tgatcgacta ttccttcttt ggttaattct ttgcaccagt gctttacagt
    28741 taccaatgac atatgttcat tatcaagcat aggttttatg tcagtatcta ccatgtatgc
    28801 attagttgcc tctatattct tagactttct cagccattca ggctgtgaac cgttagaagg
    28861 agcataaaga ttctgtgtat ctacaacagg agcaatctta gtcagtttgt tttgtgatat
    28921 gggttcatgc ctatttgctt ctatatatct ataaatttca attggtccta catttcctaa
    28981 ttttaatatc gtattgagga ctttgtcttt attccaatat ccccaggggt gttcatagga
    29041 ttttccatac aacttcttct tctgcttact tctcaacttc ctttacgcag taaaggcacc
    29101 tttactattt ataaattgtg ctgtattagt tataattaga ataagttgaa taacattaat
    29161 tcaatcaata aacgaaggac tacaataggt atatctgaat ctctaaggga tcagattgct
    29221 caactaggtc atatcggcac cacgtatgag gaggttttaa caccacttgt aaagagtgca
    29281 aagacacaga aacaggagac gacaagttaa atgaatgatc aatattttga atcagaaatt
    29341 aaagcattaa gaagtggttt agtagagtat aaacaagctc gaaaggcatt taataataag
    29401 atagcagaaa tacaacatcc aacccaaaaa gttcaggact gcattaaaca aactcacaaa
    29461 tcaaatccat cgttaactga caatgaattg attcgtgaat gtgataagaa gttagaaaac
    29521 caaacacagt cacaattaaa caagagcagg ggagcagaca agctaactga atacaactaa
    29581 aatattccaa ataaaaccga ttacaaccaa ccagatcaga ataaaattga tctgtcatat
    29641 tatggcgacg acaccgctac agcactagta aactgccgca atgaacagca aagaaacggg
    29701 ctttcagttc aagatgcaac aaatttctgt aatagtttac ctcagattga aggtgctgat
    29761 ggcgttcaag aagatgatga cacgataaag gttggtaaat tgagtatcaa acaaagacag
    29821 gagttagcgg cacgacatgg tacaggtgca gatgtcagac ttgtatctga aactggaaca
    29881 tcaggcagaa ctgcatgtgt tcaaaaaatt gaagaaatcc catcatgggt taaggtacac
    29941 gattatgtct tcactaagcc agaagctaac catatcgaag aatcagatca accaaaaata
    30001 aaatctgcaa ctgtagaaga taataggcca gctttcatga agacgagaag caatctagtt
    30061 gaaacaatgg gattacatcg ggaagaaagc aataatcaag agacagttga accagtaata
    30121 gcaatcaaat cagcaagtgt tgaaaatact atagtagaat tacctggagg tataaagaga
    30181 atacaaaaca agtttggtag ggaccaatag ataggatggg ttaccattcc aattcgaaca
    30241 actagaacaa tctagtcctg ataagatagt aaatcatgaa atggtagtat cggatctaag
    30301 agaaggacat tgctgtattt tactggcgga agagttcacc cataagacta gtgaaagaat
    30361 gacagactat gtgtattcct tgatgcaaaa gtacgctcca atatcgaagg tgtatgtaga
    30421 tggttcacaa atttcatgga ttaaaagcct taaggcttac gtaggagaag aagttgatta
    30481 tcatgaagct attgagagat ataaggcaag tcattgtgac tatactttga atatgcaagt
    30541 actgccaatt ctgttcacac ctaatgaaaa gagaagcatg ctaagctgga ccaaacaaat
    30601 gctagaggac ggattcttgg ctatagataa acgattcacc aaattattaa cagcacttca
    30661 tacctgtgtt gataccgaag gaataatcga caaaaaaact acaagtcatg atgatattct
    30721 agatggcatg tgcctcaatt gtaaggagta tcagtttaga taatgttcag aagagatttc
    30781 gttgatcttt ataataaaaa taatgaaagt tatgataatg agagtccaca tcaaggaact
    30841 aagaaagaga cgtacagtaa atacatgctt ctggaattca tcttactctg gacctaagaa
    30901 accaaaatgt gatatttgtc agcaatcatt agtaactcct gcaggacaga aggatatgca
    30961 atgtccgaag tgtggtaagg taacaactat agaggatgtc aagaagacac aagagaagaa
    31021 attggttaat acaaatccaa agactgcagg accagtactc ataagtcaga aacgtaaagg
    31081 caagtgtgat aaaccaatat caaaatatga tagtgtaaat gaacaattgg atgaagagga
    31141 taaggctgac attagagcag cagatggcac tatctaagtt ctaagttgac gactaatatc
    31201 aaatgtgccg cctgtaaaat tatctatgtt gaaaatcttc agtcaggtaa gatattaacc
    31261 aaagttcgat aaagtattgt gataagcata aacaagatgc gtttatacta gattagcctg
    31321 agatttagaa
//
LOCUS       DAOR01000066           19448 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_10501, whole genome shotgun sequence.
ACCESSION   DAOR01000066 DAOR01000000
VERSION     DAOR01000066.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 19448)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19448)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19448
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_10501"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    529..752
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6244 a         3524 c         3511 g         5945 t
ORIGIN      
        1 cgcatggaag aaaagaagta caatattata cttatgagtg gagacaatga aagaactgct
       61 agggctattg ccaaggaatt aggtataagc aatgttttgg ctgatgcact acccgcaaca
      121 aaagcccaga aaatcaagtg gctccaacat caaggtagaa aggtcgcaat ggttggagat
      181 ggtatcaatg atgctcctgc attaacccaa gccgatatag ggatagcaat gggctcaggc
      241 acggatattg ccatgtcagc tgggaatgta atactgatta aaagtgatct taaccacgtt
      301 gtttctgtat taaaaatagg cgaatactca ttaaagaaaa ttaagcagaa tttagccatg
      361 tcatttgctt acaatgccat aacaatatca atagctgccg gattgctata tggattcact
      421 cattcactta tcctgacacc aacactggct gcgctcggat ggatcgtgag tgattcagct
      481 gtcttcggta actctttatt agttaggaaa ttttattcta aaaattagnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttgttat tgttaactct ggtattcacc
      781 aaagaggata gggattatga tatgatttac tttttttcca cagaatattt gatatgttgt
      841 atgccgctaa ctgccctcat tcgtcgaatg aaggagatac tttatgtcga gcagcattaa
      901 ataaaagtgg cctggtgcat aagattaaat agcgaaaagg catattttac acaacattat
      961 ttttgttcat aaaatttcag atactcaatc aataaattag ctatggcgat tatgatatca
     1021 tcttccaatg gcaaacaaag taacttcaaa agaactgaga gatagaaaat atcaatacat
     1081 gtttgtagac gtacgagaag ccgatgaact ggaaaaaggc aagatagatg gagcagtcca
     1141 tatgccgtta ggacagctgc taagaaaggc aagacatgac gacctaaagg atcttaagag
     1201 aaagcaaatt tgcacttact gttctggcgg atatagagga aatatggctg cagatgagtt
     1261 aaacaatcaa ggatttgatg ctataactat agaaggaggt tattctgctt ggaaggatgg
     1321 ggaaaagact aagagggata acaatagtat tgagtgaagt ctggaacaaa atctacaagt
     1381 cagatagtat tttctttgga gaggaaccta gtaactttgc cttgctttgt tttaaccaca
     1441 tgaaaactaa cgatgcaaaa aaagtattag agctaggcgc aggtcacgga agagatatga
     1501 catttttgca tcaaatgaaa ttgaagttga gtcattagat tattctgtga ttgcagttga
     1561 catactggat aggatatgaa aggaaaaaag actgacaata aaagcgcaat tctttgatgt
     1621 caagagagcg cttccatttc cagatagcta ttttgatgct gtatactcgc acatgcttct
     1681 taacatgcgc ttttcacttt acgagcttca ttttatattt tcagaaataa agagtcctga
     1741 aaccaaaagg tttgaacttc ttttctgtta gaaattataa cgacaaatcc tgcggcaaag
     1801 gtgtagagat agagagggga atctacgata taaacggctt tcaagttcga ttctttacag
     1861 aaaaacagga tttaatgaaa ggctttgaat tactctggat aagagaagaa tatgaagaac
     1921 cagttaccct ttatcttgta tccagcagga aagcgtgatt gaaattttag gacctcccag
     1981 ttaatgaaat cgttgacatt ccaaaaaaag ccaatataac attggtattt caactctaga
     2041 aatatttata cgaatgataa agaccaacat tcatacatgt gcaaaaaaca aagacgatcg
     2101 tcgtctgcta ggttttcgtc attgcattct ataatcataa ttccattatc tataattttt
     2161 tgttatctgt catttatcat tattgctggc caatcgcaac caatggcagg atcagcaata
     2221 atcctgatgt ccactccatc tgcaccttcc aatgttacaa tatctaataa taatactaat
     2281 atgacctcga cttttcatca ttcttatgaa atacttatta atgattctcg gttattaaca
     2341 caaaattatc aaagcgagat tggtaaatgg cgaataaaac aatacgataa taagacaatg
     2401 atgtcggtta ccgataatta cttgccgatg tttcagaaat cagttggtgg agtagaagct
     2461 ttgaagccta ctactgaaaa ttacattcaa gctaaagagt ggtataccaa atcacttcag
     2521 tctgaaatag aaagctacag acatttcaga aactttttag aaaatggtag tccaatagag
     2581 gatgagaaat caacacaact tctatctgat gcgctaagat atgagttcaa ctcatttgct
     2641 gctttcaata atcagaccaa caaccaaaca ccttttgtct gaagcgaaat cctgcaggaa
     2701 taataatatt gatactatat ctcaaactaa atggttgaat gccagtattc tatccacggc
     2761 ttgtggacca aattttggca ttgttactat tgattaattc aaacctctac cattttaggc
     2821 aaaaaaaaat attcggctat tgatattatt attattatta tcatcattat cataaatagt
     2881 tctagaatta cgtcacatac ctatttctta ttacttaatg caatcactcc accgattatt
     2941 cctagtatcg atcctgatat ggcaagtccc attcctgtaa gacctaatac agaaaaaact
     3001 aaaacaagta ttccccagat ggttgcttcg tgtgatcttt tgttcatcat aatagcacca
     3061 atgatgacca ttattcccga aatcgttggt ataagaaaga acgaagcaga gaaccagctt
     3121 cccataactc ctggtccaaa ccacatcacc cccaagcctc ttcctcctcc catcatacct
     3181 ggtgtctgat aagaatagtt tgtaaagaac aataacggga taatgctatt caacaaccat
     3241 gttgaatatc tcgagtctat tgaagaagtt agtggaactt ctattccccc ttgttagttc
     3301 aactcaagtt tatctctgac ttctttagct ctgttcctga cagtgacttc tgttacgccg
     3361 gccgccctcg caatatctga ttgagtgata atctctccag tcttgatact tgataggtag
     3421 agtatagatg ctgcaagtcc cataggatct tttccagcgg agatttcttt tttggtaaca
     3481 ttactcatga tttctgctgc ttgtcgtttc gttctttcac ccaatttagc tatattcgca
     3541 actttagcta tacatttcat tggttcgagt tgtggtattt taaggtcaag tctggagaga
     3601 agcattctat aatcttttga tagttcttta cgctttacat tacatgctgt tgctatatct
     3661 tttaatgttc tcggagctcc catttctctg catgaaatat atatcgcagc ggctagaact
     3721 cctgaaatag ttctaccacg tatcatcatt cttgcctgag cttttctata aatatatgct
     3781 gatttctcta ctattacatc agataatcca agtttatctt tcaatacatc aagttggaag
     3841 aaagcttgtt ggagacttct ttcagttggt gtgtgaatct gagatctact gtcccaagtc
     3901 ttaagtctac gcattcttgt gcgcatcgcc acatcaagct tgttaccatt agcatctctg
     3961 tcagttcttc caatgattgt tgatagaccc atatcatgac gagccagaga ttgtacaggc
     4021 ccagttctcg ttctctctcc catttcatct gtagaaaatg tacgccactc tggtcctatt
     4081 tcctgtaatt tatcagaaat tactagacca catttatcgc aaatcatctc gccagactca
     4141 gaatctgtga ttatctggtc atttccacaa ttctgacatt tagctatggt agcggcagct
     4201 gtagtagtaa tgtctacgtg gatgtttgaa ctattattct tactattatc atctcgcaat
     4261 tcaataacta tactaacatc accataccta atatcacata gtaattacat ataaacagta
     4321 tagtaagcaa aaagttactt ggttagtgcg tagtaatcat ctatattagc tatagatggg
     4381 taactatata acccattaac taccaagcct tatcgtgggc atgaaagctg gtagatcctg
     4441 ataacgatga cgtcagaaac agacagagat tcaataacaa taaatccgac acctaggagt
     4501 gattctaaga taagatgttg cccaattaac aatacgctta atatcattgg gaaacggttc
     4561 acgattttga ttctaaggaa tatgataaat ggcaaacaga ataggtttaa ccaattttta
     4621 aactccattg aggaaagtaa tccaaagact ctttccgtaa ggttaaggga aatggagaaa
     4681 tatggattga taaaacgtaa ggtctatcct aacgaaaaac ctgtcagggt agaataccat
     4741 cctgttgaaa aaggtctgga attgcaacct atacttgata tgatggctgc gtactcttta
     4801 aaatattgta gtaaggatgt ttttaaagat gcgaagccaa gagaattcag agaagtatat
     4861 ggaagagaaa tggctaaaat ttctacctaa aaaccctcat cttaggctct agctttggac
     4921 ctattctgat ttcatcatta tagtgttgtt tagaaaatag taggtttgac ctgcagagat
     4981 ttcacttcct tcagaacatt atggttcctt tttttctaat atgggtctga ttcagactaa
     5041 aataaatgat attctacaag atatgcattt aataagacat ttaataagac ctgaatattt
     5101 tgctactgca aatcatctac tgctactcat aacttaaaat ttatagattg tggtggtctt
     5161 ggattgagga taataaccaa ccttggtacg gaagcaataa cgtatactgg cgcaatagat
     5221 ggttacatta gtgttattgg atttaatccg actaagcaat tagggttagt aatattatgc
     5281 agctgcgacg aaaaggatat tacttcacca gaaatatggc aaaaattttt agatcttcca
     5341 ctactatttc tgcttcatcc agagtcaggg attcaccaag agtagttatg ttaggacatg
     5401 ggtttcgtgg tttcctagtt gtatagaaat tagtttgaag tttaatcatg agctttgttc
     5461 atgtttgagc cgtgtagcgc atgatcgttt ctatattaac ccttattcga aggtgtattg
     5521 gatggaagat tttgatgacg tatagtaata ttgcctgtat ggcttgaaag ccatgcataa
     5581 agatccgcta aaacatattc ctgtataggt cccagcgcgg taatccattg agacgaaggg
     5641 tagaatacat gtcctagacc tgggtatgtt atcaatgtgt gatcagggtg attcaattcg
     5701 gttaatcttt gctgcaaaag aatgcttgct gaacaggtgt ttgactatca ttttctccat
     5761 tgagtattaa gatatttgta gaagaaagat taccaataac gctcagattt ggttgtaaag
     5821 caaggactga tttaatccat gtacggcatg gttctaaagc agtacactta ccagttggga
     5881 acggagcaag tgattcaaat gtatttacta gcaaaggttt taactcttta tcactgttca
     5941 ctgtcccatc cttgtctacg tctaagccag gatgccaaac atactggcct gttttgttat
     6001 ccttttctat cagagaattt acagcttctt ttgtggctgc atctgctgaa cttccattaa
     6061 tccttgaggc tacttctgaa agggacaata ttccatcatg atctaaatct aatatctgct
     6121 tagcaaaaag tagtggttca tccacatttt gtaagcgtat aatatcacga aagttctggg
     6181 caactgtacc cataagtact atattctcca ccttagtggt attctcaatt gcaacccttg
     6241 gaacaataat tgtaccttca ctatgaccaa ttaaagttat tttcttagga tcaacttctg
     6301 gctgctgaat gaaaacactt agagcttttt ctgcatcttg ttttagttga ttggtcgtta
     6361 gatttcccca tacattgctg tttagaatag ttttgttagc acctatacct ctcttatcat
     6421 atcgaagtac tgcaaagcct ctttctgata gatattgatc tatttgccaa aatggtttag
     6481 gtggttggcc accactttta tgaacatacc ctaaagtttc gttcttatca tgagcaccag
     6541 aaccatgtac atgaagacca ccaggaaatg gacccttacc cacggcaggg agagttgatt
     6601 gagcactggt tttcaaaccg tttcctaagt ctataactaa atttctatat tttatagtct
     6661 ggatgtattg ttgagctagt gtagtctgtt gttctctgaa aataccagtc gtaagtaaac
     6721 atgacaatac gagcatgatt atcaaaaaca accctacaga aaatgtcata gttcttgcct
     6781 tatgcatttt aaaaaagtca cttaacacat gaatataaga tttcataccg aactccaaga
     6841 tcttacgccg aacccttata gataaactcg taaatgcatc cttatcaaag aactagagcg
     6901 cggccaacat ttgacacaga caaattggtg cctcgcagct atacgaattt agataagatg
     6961 acaaaaaagt acataacctt ctttagtttc aactaattgc aagtggaaca aatggaaccc
     7021 aagttaaaaa ttctgggttt tgcaggaagt ttgagagtgg gtccatataa taaagccttc
     7081 ctacgcgcgg ctgctgatct gctcccagac gatacaactt tggaaatctt cgaccttaat
     7141 ggtattcctc cgtttaatca ggatcttgaa atggatatgc ctgcaagggt aaagcaatta
     7201 aaatcaaaaa taagagaagc agatgccatg ttgattgcta ctccagaata taattattct
     7261 gtacctcgag tcctaaagaa tgcgattgat tcagcgtcaa gaccatacgg tgataatcca
     7321 tttaatgaaa aaccagttgc aatcattaac gcatctattg ggatgcttgg tggcgcgagc
     7381 gcaatatcat ttacgtcaga tgtttgcgtt cctgaaatgc atccagttaa tggcctagag
     7441 gtgatagtca cgtttgcaca gaataagttt gacgcaaatg gcaaactaac tgatgagaac
     7501 accagaaact tcttaaaaaa actacttcaa aaccttgcta tctggactaa aaaattggtt
     7561 catacagtat gtgaactagg ccaaacctaa acgcgtattc taaaccatat tataattgat
     7621 agagtgattt tattatcaat gtccaagaca tatgaagaac tcagcggcta tgcaactccg
     7681 aatggaacaa cgaaatatgt agattatgca acccaagaga aaggtaaacc tgctactcac
     7741 tttcgtgttt ttgaagaact atacttatca agcataggaa ttggaactta tcttggtgag
     7801 atgactgcag aagacgacaa agctgttgaa aatgcagtct atcaatctgt caaatcaggt
     7861 gccgtgaatg tcatagatac tgccataaat tacagagcaa tgagatcaga gaaaagtata
     7921 ggacgtggat tgtctcgtct gataaatgat ggcattattt cgagagatca agtatttata
     7981 tgtaccaaga atggctatgt aacaaatgac ggtgattatc ctgcaattga ggtaatggaa
     8041 tatgtacaga aaatgtatgt agctacagga atcatcaaac cagacgatat tagttctggc
     8101 tacaatgtac tcaatcccgc gtatattgaa agatgtattg ataaatcact acttaacatg
     8161 catctgagta ctattgatct ggtttatgta cataatgcat ttgagagttg gtatgaagat
     8221 gttagcagag aagaatttat gcagatgttg gcaaaggttt ttgaaattta cgagaaatac
     8281 cgttcaaata acaagatacg gtactatgga atggctacat ggacatgctt tagagtgcgg
     8341 ccaggcgaca aggaatattt atccttagaa gatgtagtta aattagctga aaagattgga
     8401 ggaaaggaac acggctttcg ttttattcaa cttccctaca atctagctta tagtgaagca
     8461 ttggttctaa aaaatcaaac tatcggtgct gaaaaaaatt taaatatact agaagctgca
     8521 gcaagattga acataggaat atttacaagt atcccattat tccagggacg cttacttaga
     8581 gcaagtattc ctgactacgg aggattaaac gatcaagttg caaaattaat ccaaataatt
     8641 cgttcaagtc cttctgtcat tgcaccatta ataggtcaaa aaaaacccga gcatgttgag
     8701 caaaatctta aaatctcaga cgttccccct atgaatgaag aacagtacaa gaaaaccatt
     8761 cagatgctgt taaagggaga gtgaacaccg tatattggcg cagttgtatt agattattcc
     8821 tcgagtaagc ctgaggaata tagggggaac taaaagctga gtgttcattt cagaggcagc
     8881 ccatgctatt ttcctacgcc ctttaagagc cctaattatc gttcatcttc ctgccgtaat
     8941 cgtcttagtc tttccttctc ttttcgacta gtaggtaaat atcttaattg cattccatag
     9001 catggacata acataccacc gtaatagagt ataatacttc acattgccta caataatcta
     9061 tgaggtacgc gctcggtcta taatcaaacg gcatttggac gatggtcgag gcagcaagat
     9121 tttctagttt tacgaaccaa aacttcaatt tacaagactt aaactagtac tagcttcact
     9181 attccgtagg acaggagacg ggatgttatt ttctctaatg atgtattgca ttggatacat
     9241 gaccataaaa aaagtgtttg aacatttttg gaaaaagcta agaagtaatg ggcagctatt
     9301 gattcagtgt ggaggttatg gacatttaca aaaaatttac gtcctcttaa acaaattaca
     9361 ggagttaata agttcagcga gtatttcaaa aactgcgagg aaccctggca tttcgcaaag
     9421 gccgaagaaa cgaccgatct attagcaaag acaggattta agaatatcga cgtctcccta
     9481 tctaatgagt gtgctaggct tgtagaacac aagtgatatt cttcatttat gaaaactgta
     9541 gtaatgaaac cttttcttgg actatctccc aacctatgaa ttaaaaaata tatacctcga
     9601 gctagttata tataaagtct gccactacca aaaataataa agaattatct catccttgag
     9661 tattagacta tgttaggcta agcattacag ctaagaaaac ataacacggt aggttgctcc
     9721 ttccctattg agggtttcat ttgctaatcg ttaatgctct ctttctaatc tcgacatcat
     9781 gtcaatttga acataattcc acgaccgggg aaaccagaag aacctgcgct cgaaggttgg
     9841 aaaagctggc agtaaatgac agtatctggg aataccacgc tagctctttc caaaaaaatt
     9901 aaaataaatc gctaaaacac gtagtataat gagtcctcgt caagactttc ttcctctaac
     9961 tcatggtagg caggcattcg gctgctctaa ttgtggaaca cattatattg tatttccacc
    10021 aacctcagaa tatatttcta ctatgcttga accttgtcca agagtcgatt atcagaagag
    10081 cttctatgac tgcataaagt gtaaacacag aaatatgttc tattggcata aaagacatcg
    10141 tgaagacaga cttttgtttt ctgtataaaa aaatagttca gtttttttat atgaataatt
    10201 ctgaagctta tattagataa tgttattgga aataatagat aatactcatg cctcatacat
    10261 catacatcac tttaatcgct acgcccccaa aaccactaaa gctaccaccc cacggtagta
    10321 gaaggaggaa gaaaagcagt attaattact tcaatgccat atcctaccca agttctatca
    10381 ctgtctctaa ttaaataatc tatcaaatga aaaccataat tgttaacaaa tccgtgattt
    10441 ttaaagacta cttcaaaagt taactgatcc aatattggta acaatggtat cttgttctaa
    10501 cttggatcta aattccctgc actccgcact tgttgttcga aagaaaggag cagtcattcc
    10561 aagagatata cagctacact ttcatggcgt aagttgcaag ataatatggc aaccaacaac
    10621 tagtgttaat atcagaccca cagaaattgg aggaagattc gtagcagata acgcaaacct
    10681 cgaaatatta atctaatcta taggaattaa tataagcctg gtctaaaaat aactttgaat
    10741 aagctgaata agggattggg taacaagaga aatcaaaaga ataaaaggga tgacagttac
    10801 gttgcgatta atagacgtag gagaaagcgg aatatgataa tgataataat acctattgta
    10861 gcagctgtta tagcatttgg agtcagttct acttctcttt attctacaca aggaccgaaa
    10921 cctttagtgg aacatatcca cccgcacctt actgttactt ttgatggtac ttccactcct
    10981 gttccatcac aaatagggat agattcatca ttatggaagg ttcattctct agaccaatat
    11041 ggtacaccag gaattgctcc attacatacc catgacgcca gtggtaccat tcatgtagaa
    11101 tctacagcaa acagaaatta tacgctaggc gattttctag atatatgggg aggcttagat
    11161 acaaatggta agacagtccg agcgagcgtt gacggtaagc cggtctcaga ttttagagac
    11221 attattcttc gagacggcga gcaaattaat ttaaatgtcg gaaggtaaaa gtaaaaaatt
    11281 tatattttaa gataattcag attaagacag tgaatctgta tcttaacgat aaagaagaac
    11341 aggccagaat cattctgata aaattctaga acgactctac caaatgccct gaatatcatt
    11401 tttacgtgct cccttattac tcttgacaat atcatagtgg tagcagtact cggcaatgaa
    11461 gcctccgatt gacccaatgt ttgagaaatc gatcttttga aaaaagcaac aacatacctg
    11521 tgcagtccca gtttatttta tttcagtccc cagaagtgtt tgctatctcc ttacttatct
    11581 actatacttc taataacttg ctttcatcaa aagattttga attataccag ttgtatctaa
    11641 aataatctag acaccattca tgaaataatg ggtcttcact atagaatact gaattcatat
    11701 ctgtctctcc ttgtcctttc gctgtgggga acataacagt tgcttgcttt tcgtttaaga
    11761 ctattgctac ctctattcga tcaaccatcc ttctttctac tatccctttt tttatgaatt
    11821 catggaagcc agcgtttttc aaaagttcag ttcgcttctt tggcaccata gctttttgag
    11881 gtagaatata attaaagtgg ataccaccat gttcttttat ttttggtatt actgcttcaa
    11941 tcaaatctag tgggatttgt ggtaacattc cgtatatgta ctcggtagat tctctgtaaa
    12001 tatgctgttt ccatagctca ataacagcta ctaatcctga aataaactcg cagttattca
    12061 aggcgccaat tctctggatg aatttcattc gagtttcttt tccgaaggta tgagttgaaa
    12121 aatattcttt atttcttgac aaaaaatcaa aagttgcaat ctgatttatg atggtatttc
    12181 cgaaagttgt taaggaaaat attcccgcgg aatctttttt gatcaaccca gcatccatta
    12241 atctgttaag atttctatga acttcttgca tagtaacatt aagatgcttt gcaagttttg
    12301 aaaggttgta aactccggtt tccgactgtt gcctatttag tttaaacagt atagatagcc
    12361 tctgatcact agctagctca aagaagatcc gttgtgtact ttcaatgtgg cgactactgc
    12421 tcctcgagtt agttaaatta gcgtcatctg aatgcatgta tacctatagt tatattactt
    12481 tgtgaaattc ttttctattc tatattgttc ttattctcag attctaagcc atcaagtaat
    12541 aatagaggaa taaataaatt actattggca gtcaaaaaca tgtaatacta ggactctcaa
    12601 atcgtggtat gctagactac tacaaaatat tcaaagaaga gatgcaatta atcaacgaaa
    12661 tgaacagaga cagtacgaac agctgaattg tcttgctaca aatttcataa atgtctccaa
    12721 ttcattctat agcctggaac aatggcgtca gacaactatt tttcatattg atcttgtctt
    12781 cgtcaaaaga atagaggtat cgctatagtt caaacaaagt caggcagagg tgcggctatt
    12841 atctttactt cgaactgtta gtagatacta ttggccgttg tcttggcagg ctaagttatc
    12901 aaaccggtgt cccactcaca catatcgaat tcaatcatcg cttggacttc atgattcgca
    12961 ttcaaaaatc aatattatct ccactctaga atggtcttaa ttaattatgc aatgacaaat
    13021 ttcaataagt actttgaata agcaaaatta ttagaatcga aatatgatga taacaatcct
    13081 aagtgaaata tactataata accaagatca ttaacaatat gattttggca acggtagcac
    13141 tagtattatt cacagctatg ttgatgtcgg ctgcgttagg ctattcttac aaggcggcaa
    13201 atgcatcaag tttcacaaaa aggagttcta ctaattgtga aggtgatgtg tgccactcgt
    13261 taatatgtat cagcgaaaag tgctatagat cagtttcagc tttaaactcg actcagcaat
    13321 tgttaaattc gactaaacct taaggagcta ataggtatta taagggcaat tcttctgtgg
    13381 tatttcaatc atatcgagca tagcggattc ggttgttcta ttcttgaaac gaaattgaaa
    13441 tgtcatcgca tgagcgcgaa attacaatag cagcaaccta gaatttttcc tatgctaaca
    13501 aactagcatc agtgtttgtc agactttctt attttgcgtc tggtaagtaa aatatttcat
    13561 actagttagc aacgaagacg gctaagatta ataccagacc agaaagtaaa cgaactcaag
    13621 acactaattt taagtgagca aaagactaat gagtatttgc aaacctgacg ccaacttggt
    13681 ttcctttatg taatttttaa ccttgtcatg cgaaatccct aatacgatgt tctgatatgc
    13741 cgaggtgtat tcataatatg tttctcataa actttcggta cgaagaacac tgatatatag
    13801 tatgcatgta ctcttactta gtggttatac gataatgtca gacaacgagt atcgcaaact
    13861 atcagaagca gagattaaga atgagctcgg caaattagat ggatggaagg tggtcagcgg
    13921 taagctaagc cgaactttgc aatttgagaa cttcattcaa gcgtttagtt ttatgaccaa
    13981 agtcgccttg gaagctgaga aaataaatca tcatccagaa tggtttaacg tttacgaccg
    14041 gcttgatatt aacctaataa cacatgattt aaatggcata agtacgtatg atatcaaact
    14101 ggcacgcact attaaccagc tatatttacg atagatatcg ctgaacatta tttttggtac
    14161 catacccgga aacaagtcat catggcatga ttttaactaa tcattaattg gatttggtcg
    14221 tgttctgatt gatgttggtt tacttaaatc agcggatgtt gaaattaaaa atatgaatga
    14281 agggaaagtg ggagcaccat ttcaatatag caacattcag atccaccgcc aacaacaaca
    14341 aggacctttg ccgatgccat caatatgaca gattatagtt ggtgagagta ggtatggtgt
    14401 agataggaaa tcctatggca gaaaagaatg gccgtcatag tcggggcaag ctaccgatca
    14461 tgaggtacag aacaaaaaaa tagaaatatt ctatttcgga ttccttttgg tgtatgtaca
    14521 ttagcgggtt ctgacggttc tctcatatta ctcgatgctg atagtcatat atcggcgtag
    14581 gagatacggc tctcagtgcc tgataattta catcaaatgc cgtgataaaa caaacctcaa
    14641 catcattttt tatattcctc atctcttcat agttgcttca aatggaattg aagtcgaagc
    14701 attagactat tctgtcatca ctgttgagat actgaataag ataacaagga aaaaaagact
    14761 ctcatataga tgatgactaa agattctatt acccattctt atcgcgaata gaaccggctg
    14821 atcgtgatcc tattattaca tgcttttgaa aaaataattt aatgtgcaat tttttaatct
    14881 ttataaccta ttacagaacc agctaaatat agtccgataa tttcgccatg aactggtttg
    14941 tgcttgtagt ttgtctgcct aagtaactga gaaatcagct cgataactca aataacgact
    15001 cttacttctt tctcttcatg tctgccgcga tctggtttac gattggtctg caaataattt
    15061 ctacgcattt tttatatacc tttatctccc gacaatatcc tcttagtaaa gcaatatgct
    15121 gtggcaatat ggtagtaatt tttataacct ctaacatctg ctgtgatagt agtaatgtga
    15181 tacaaacccc taagttactt gcagataaag tagcggtagt cactggatct actaaaggta
    15241 ttggtttttc aatatcaaaa gagtttgcag aaaataatgg tgcaagtgtt attgtttgct
    15301 ctagaaatga ggagcaagca gatcgtgcaa taaaacagat caatggtaaa gtatttgctg
    15361 catcgctcga cgtaacagat aactctagcg tacaaaaatt cgtgcagcaa attttatccg
    15421 cccatgatag aattgatatt ctggtaaata atgcaggtta tccttttgac agagaaattt
    15481 ggtataaaag gtttcatgaa gttacagatg aagagcaaga aaaaatcata gaagtagatc
    15541 ttaagggatc tgtacgctta tccaaggcag tcattcgttc tatgcttcaa aacgttctca
    15601 acagaaatgg tggggtgata ataaacatct cttcaactcc tgcaattgcg gggcatatag
    15661 aaggttcccc gtacaccata gccaaagctg ggaacatagc actgacaaaa tgtattgcaa
    15721 tggaatatgg cagcagcaac attcgcgcct atactttggc tcttggcaat atagcaacat
    15781 tggcaacatg tggctcaatg actgaatatg atagagctca ggccgctgct gaaccatcaa
    15841 tgaaaagatg gggtaagcct gaagaagtag ccaaagttgc tgcatgcgtg gcaagtgata
    15901 atttctcttt tgctacagga aataccatta taatagatgg aggaactgta ttattgggat
    15961 agcacctaca cgaactcccg cggagcgtct atatctgaat attcgtagga tgcattgtaa
    16021 atcaaagtag gctgaaacat gaatagtgat gatgctaaga actaaatctt ttgctgtttt
    16081 ttaagcatct tctttattgg cataattagt gattctatat gcttttctat gcctacaaaa
    16141 ataagagaca ctccaactcc caataaaata ccgcctatta catccaatgg ataatgacca
    16201 ccaacatata ttctggatag acatacaaga gctgcttcta tagccaaaat agcggatacc
    16261 gcaagtttcc taggagtatc tctagataac attaaagcta cagctgcacc tgctgatact
    16321 atcatcgcat gaccagaagg aaaagcatac tcccgatccg gtgcaattaa aaagtctgat
    16381 tgtgggatta ccggtctagg tcttgcaaca atatcttttg ctaatacacc aagtggtaca
    16441 agaacaagca ttgatattgc aataactact gcagtctttt tccctgtcca accgcctaaa
    16501 acaaataaaa gtattattgc tataggccag aatatttctc ttccaaattg ggtaagccag
    16561 atcatcattt gattaaagac aggataatgt gagttgttaa tgtccaaaaa tgctaaagaa
    16621 tctaccttac taatagggga attattaata ccattattaa aaccgacttt tggggagact
    16681 aatatggcaa ttactatgaa tagagcaagg ataacaattg aagctccaaa gtatttattc
    16741 actgttgtat actcgtctga caagtactta taaagcatac ctctctatgg cttgttctac
    16801 tgagaaaaag gtactgctga tagcagttat tgcgttcata tatatgccta ataactcaga
    16861 caaccatgat gttactatta ttgatttctt gtgcttgaac tcagggattt accgatagaa
    16921 tgttttttag tttttctaat tttatatgac atagaatgtc caatccagaa gatattctct
    16981 ctagaatgtt tccaaaagat gttcttccca gattgtatcc aaaagatgct cgaattcaag
    17041 agataaatga gatcctacaa accagtatct ttaatactat gctaaataga aagtcgcaag
    17101 accggagcgg aaaaactctt ttttattagt tatgttttct caatagtaga tacaattgcc
    17161 cttcaaatgc aagacgtgcg gtttgcaatt tgatgagaag agacgtcttg aaatccacaa
    17221 acaagtacat ggtcggaaat ccaaggtaag cgaatacggt agcccggaat ttagtcagga
    17281 tagattgaga ggatgatttg attcagctcc taaacaacaa cttggagata taaaggcgtg
    17341 tatctctcaa cgtgcattga gctcccatca atcctaacag atcaaacccc tccgtagatt
    17401 ttgattctat ctgagctata gatatcaggt gaccatcatg gtctccaatg ataacggaat
    17461 tttgaatgac acatcctcat acatgctact actgaaacac tatctagagg aagaatttat
    17521 aggcgttgct gacaaaatag ataataataa gtaggttatc atgttgagaa taattaatca
    17581 gtagtggcat atagtgacct caataaaagt acaatataaa gagacttgct cagtatattt
    17641 gacttcctac cataccccta cccataactt acgtacctac atctaatggc tcttcgaagt
    17701 tttctagagt aattatctaa caagagaacg gtagcaatcc atgctaaaat aaaggacata
    17761 accattagtt ttccatttgt tcaaaatgtt agtaacaatg acaccaagcc tgacaatttc
    17821 tccattagct aaagtttcta agatgattct tgaagcccgt tattaagaac ttaatcttcc
    17881 taccttcctt caaaccaata gaatcatttc accaacctga gcttcccatg accagtccaa
    17941 cctgccacct tcattatcgt atggttccag cagtgtcatt caattgcttt catatgattg
    18001 ctttatcaaa gttaatgtat agccaatatc agaaggttct ctaattatta tttcataatc
    18061 gccatttccc caatggccca cattcgatac atctcttgcc actgcttttg catcgttcaa
    18121 tgaccccttc ttcatattta gaaataacgt aagatttgat tttcttacga caatgtctag
    18181 aaagtttctc ttgcgaataa atgcaatgta ttttgcattt ggttttatag tcacgtctgt
    18241 tgctatagat aaaattcctt ccttgagatc tttgtagaga ttatgtattt cctaattgat
    18301 tatgtatttc ctaattacat ccttcgaaat ggttatcttc attatacact cttacttgtt
    18361 gattaactga ccgtacaagc gcgctcttct gacttatttt ggtaatagat tcactccttt
    18421 ctggagactt aatttgattg aatagaatag tattattaga atataatctg gcctcccata
    18481 attccattgg caaatctttg aattcaatgg cctttctctg atatgtagta aagaaaggag
    18541 atatgaaaat tactcttgag tctggaatat tctctcgcct tcttccgctt cactctttgc
    18601 ttcaaattct tctgatttcc cgctcaggcg ataaatgttc caaattcctt tgtcagctct
    18661 caactatcag tgctatatga cccgacttct agatcttggc attgatcgta ttcgaaatat
    18721 tattatggac atggccaaac tatctgagaa ttcagtgttt actgcaatag aatcatatga
    18781 aaggtatcga tgtaaaaagg caaatttttg aatggtcaga aatacttcga gtactacaag
    18841 aagaagtagc cgatctggca atagaattga tagcacgtta tcagcctgtt gctggggatc
    18901 tcagattcat aagatcctgt atggagattt cttatggatt ctcaaggttc ggacgctatt
    18961 cttatgatat tgtagatgta cttgagacaa tgggctatat ttttgactgt gacaaagctg
    19021 ctgtcttaga aatggcaaaa aaggtaagag aaatgattct gctaagcgtt gctgcattgc
    19081 agtcaagaga taaagacgca gcctctaaac tatacgaaat ggatgataca gtagatgcgt
    19141 tatactggaa atacttacga aaagttatta ctccattaaa aaatgaggag gatgagaata
    19201 aactagagga gaaaacagaa gtcattactc gtcgttgtca tgcgtcctgc ctattgattt
    19261 taagatatct ggaacgtatt tcagaccatg cttgttatgt tggtgattct gtaaattata
    19321 ttgtaactgg cgtatcaaga ccaagacgat gagtagtatg gtagtagtat ccctaagatt
    19381 gaagaatcga ttaaaatgtc ctttttatag cctaagaata ggtaatactg attttgatgt
    19441 ttagctac
//
LOCUS       DAOR01000067            6150 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_70351, whole genome shotgun sequence.
ACCESSION   DAOR01000067 DAOR01000000
VERSION     DAOR01000067.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_70351"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    462..599
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3374..3539
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1709 a         1077 c         1191 g         1869 t
ORIGIN      
        1 gggttgatca ataatcatca ttccatacaa ccctctgttt atgtgctctt caacaggggc
       61 catatggcag tgatatggat acacgccgta cggctgtgct ataaagtcat gtgtgaactc
      121 agagccgggc catatcattc cagccagtcc cattactcca tccatagctc cgctgtgtat
      181 agagtgtaca tgccacgaat ggggatgcaa actgttcttg ctgtttatta ctgtcactcg
      241 aacgtggtcg ccctgtgtca tccgtagtgt tggtcctgga attgtctcat tgaaagtcca
      301 agccgggaat tgcagcccct cacttgtaat cgacatattg tgacccatac catggttgtc
      361 atcgactatc agcgtaaatt gccttagagt tgtaccattg ttaaggatgg ttacatgacc
      421 acacgaaaaa tgagtcagat aagttttcca tgttgtagga gnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
      601 tgtaggagtg tgctgatcac tagcgcaagt cttttcttta tcgtactgga tcacattttg
      661 cttgtatgtt gaagatgttt gtaacgcaaa cagaaatgtc gttgcgccat aaacggaaaa
      721 cgctccttga agacctccca taatgagtgg ggagatcaac aacgtcgcaa acgcaaatgc
      781 tgataagatg atgtaattat tatttttatc taacatctca atgatactac atagggatcc
      841 accgtaatag atccaaagtt gttgatgttg agatggtaaa ggctacagac ttgaattggt
      901 gaagagttgt atagggggca acaatcacat tttttgcgta attctcaatg ccaaatttta
      961 gcttcaattt gaatatactt acagcatata aacgtgtact taacgagttc acctgttaag
     1021 tacacctttt tcgtacataa gaaaacagta catttgagat ctcttaggac attctaaaac
     1081 agctctgtgt ttattttatc ttgagtccta gtcctttccc tcgctatcgt attgacgcac
     1141 atcttgatca ttgtgtaaag ttggaataca acgtccatat tctccggttg gcgtcggcca
     1201 aataggcttt ccttaaagaa cagggcatgt tcgttcttga gttccactaa gcgagtctgc
     1261 tgtcaatcac accgggcatt gttgtcgcgt agaaaaacgg gtctggggga caatacttct
     1321 ctattggatg ttgtgcagca ggtacagaaa agccccactg ccaccatttt gttacccgtt
     1381 gttggtatgt gcctccagga gatgagttaa tagataagaa tatgtgcgga agcataccga
     1441 acatcgataa cggcttgcgc ttacagctat gccaatttta ccaattgtaa tacggagtgt
     1501 tgttgcgttc gtacttgggt tattctaggg aaagcaactg taaggttaaa tgccggctag
     1561 gatatacaag tgaaaatact tatcatggca aaaagctgtg agatctgtgg gtcaaggttt
     1621 ggggaccatg tttgtgctta ctgcactaaa tcagtatgtt cctcatgtct agttcaggat
     1681 aagagcatgt gtataaaatg tggtaggatg agaaagccag catccaagtt ccttagacga
     1741 aattttcttt ttataatgct atttgttgct ttttggtttt ttactgccgg ggtttatcca
     1801 ttcccgtact tccttgccat aggcaaatca ttggatccag taattatgga gccaatttta
     1861 attgcaacag gagtaatggc cataccattc acattcttag ttatcgcatg gaaaaaacga
     1921 cctccaccta aatgacttgg ctgtggtaca aggtgaatgt cgattttttg ctttttctcc
     1981 tactatgatt tattatctga tgactgagct tcagctaatg aatggaaaga ccaggtcgac
     2041 tgaaatgaag gatcagtgct tgtatgcctc agattttcca tctactaact ggtcgtacct
     2101 gctgtagtag taacagcacg tagcacatat gtagcagttc actgcatgta tagtgtactt
     2161 ctcgctgtat cgtaccccag gttggccata gtcaatacct taaaaccatt gtcatgtagg
     2221 tatttcatta attgatcaaa taggcctaca ttagtgatgt agggctggtt ggtggctaaa
     2281 tcgacgtcat gaaatgtgat tagaggaatt gcatttatta ctccgccact gttgtattta
     2341 ctctggctgt ttaccatctt aatgaaatct gcgaatatcg ctgcattgtt aaatgagttc
     2401 tttatttcgt ctacatcaaa agacaaactt ctaacagcat atctatttgc ataagtcagc
     2461 ttaccatcag gtaagtatgt tctacaatct ttctgaggtt gtttcttgta tccgttgcag
     2521 tgtagaaaca tcagtggctc agttccagat ctagccatat tgtagtattt ggctacgaca
     2581 ttgactacag tggcattatt tgatccttcg ttatatggat aagcaaaggt tgtggcatga
     2641 tatccatgat ttgcaagaca ttgtttcgat ccacctatct cgaaatttaa tgcatttgca
     2701 gataaatgat ttagatggcg atggttcata gtatgtgatt gtatatccat tccatccttc
     2761 tgcattgcgg ctatatcttg ccagttagtc ctgttgagga atctagtagt tccgctaatt
     2821 cctgtcttgt tacatattat gaagaatgta gccttgaacc cgtatttgtc taaaattggc
     2881 ttggcgtata tgaaatcgcc tcttaggtta tcatcaaaag acagtagtac caatttatta
     2941 aaagtagtac caaccccccc aattcctgtc ccgttgatag atcctctctc tttttggatt
     3001 agtgacgatt ggtaagtgta ttggctatga gcttcaagtg gcatgacagt tattattatt
     3061 attgcaatag cgctagcgat taaagcttga ataatccttc tacttttcac taagattgtc
     3121 acaacggcta atctatacca taggtagtgc aaattataca ctatttaatt tataccatag
     3181 tgtacgacaa atcatgaagg caacttgcca aaaaatatcg aatattcctg ggtattagac
     3241 ctatccggca agacggttga ttgaaaaaat gcaaattttt gtatattcat gcactctacc
     3301 gagaatggtt tcgctttatt acggttagaa attgcgactt gtcattgatt agttatcaat
     3361 gacaagtttg tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3541 ttagaaattg cggcttgtca ttgttaacta atcaatgaca agtttgtccc ttcatttagg
     3601 aattatttga aataaattgt ggctattgat gcgcctgaga aaataagtta ttatgcgcga
     3661 agagagatta ttgacgtgtt tggttaaaaa gctattgaga acaaaagaat tcttgtcatc
     3721 gcttcctcaa tctgtgctca aaggtgaaga tattctgatt cctgataagt tagtaaggaa
     3781 attattcaga tttgcaggtc ttcgaaggtc agatatcttc tatcatctag ggtgtggaaa
     3841 tagcagcgca gttgcaatag cagccaagga atttaaagta aaaagatcgg taggaatcga
     3901 gattagaaga aaagttgcaa ttgaggcgcg cagaaaagtt gtttctttgg acaatgccca
     3961 aataatcatt ggggatatta gaaaagcagc aatttctgat gcaacggttt tatttttctg
     4021 gttcacagat cctagagtaa tcccaccgat ggtaagcaga tttcgaaagg agctgaataa
     4081 cggtgcacga gtaattacaa tattttctcc ccctgatctt atgctaccaa caaaggcaaa
     4141 atttccattt tttgtgtttc agaaaccatt caagtatgct aagcggattc aagagcaaat
     4201 tgaggtaatt tttggaaaac cctgcattga ttttgttgaa tcgtggttga tggctgggaa
     4261 gtatattgat gaattgggtg tcgttccgga ccaacaccgg agatttgtga atatgcttca
     4321 aagcatgata atatggataa atgcctggaa taccggagtt gcttgcgaaa gcgaaattcc
     4381 tccccctgta tattcgtacc ttggaatact aaaaacattc ttcgcgatag atttgtcaga
     4441 tttgatttca aaggaaaaat aagaagtaag caattcctac tctgcttctt tcgtcgttag
     4501 gctttttgac agcgtcagcg tacacgagcg attagaagaa caattttatc ctgtttctag
     4561 ctaagtgagg aataatgtca atgacaagac attaacatca tcatgcacat tgacattagc
     4621 atagttaatc aacaattttc tcaaaagtca aggcaaggaa atatgtccat gcttattata
     4681 ttagcgtata acaaataaaa aatgacagga aaactggtct ttagtacaac ctttttgcca
     4741 tccatgaata aataagcatg tgtaaatata ggtcctcgtt tttaatttct gttaaattgg
     4801 attttgtttt gctgggtgag gctagtagac cgaagatatg actaacatag agaactcgat
     4861 tgagttgcgt aaaagtggct taagaatcgc atttgtaggg ataatttctg tgatcctagc
     4921 tttcatattt tactccagat taactatccc gggtgaacat ctagttatgt taacaccgga
     4981 gctaaagaca ctcgcaagcg gtgttcttgc aataacattt gcttcaatgg catccattac
     5041 ttacggaata agtaaaatat ttaaagccga gcaacaagga ccgatgaact ccaatagtct
     5101 actgtattat atcaccaatg cgttttcgga taataaatat tggaaagcta tggtaatagc
     5161 tgcaatagca tatggcatat tttttggatt tttatctcaa atatttattt acagaaacga
     5221 catatcattt agccaacaag gaatcgtaat cccatccata aacataattc cttgctgcaa
     5281 tactcccggt tacgtaccga tgttctctgc ctatcttaca gatcattttt taatcttgct
     5341 aataccccta aatattattt tagcagtcat agtgtctgcg ttggtaggat ttaatgcttc
     5401 tctaggtctc tatgcattta atttatcaag aaaaatacaa agcaccaaga agctatcttt
     5461 tatcggaagt gtaggagcgg caagcggatt atttgttggc tgcccgacat gtgctggtag
     5521 tttcttttca gcacttttag gatttggagc tgtgggagag agtatatcgg tgttagcatc
     5581 atttcaaact ctgttcattg ctttaagtat cccagttttg ataattactc cattccttgt
     5641 tgcacgttca attcgaacaa attgtcgtac ttgtaatggc gctgttaaat taaagtaata
     5701 acaactaatg aaatacacca cggacacgca cttttagcca agctagaatg ggaacaaagg
     5761 gaaaagacga cgttattcta caaagaattt cacatggtta gtgaatttga aaacaacgct
     5821 tggcaattaa gcaggcaaga tagtgttatt tctgtatcgc ttatgagggc gtcacacgct
     5881 tgaaactaaa atgtacggag ttgtctgagt tgcgtatact atctgtttaa tatcttcgat
     5941 gaacatgaag aaaattgaag acagaagagt attatcagac gctttcataa tcgtaaagcc
     6001 actcaccgaa tgtttgaaaa gcttcctgtg ttctacatca tcttttttgt tggctaataa
     6061 accgcaccag tcagcatatg gttgcaaaat ataaagtggg tcggatttac cctctgcagg
     6121 ttttaggcta tttcctattc tagccagaac
//
LOCUS       DAOR01000068            7672 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_19818, whole genome shotgun sequence.
ACCESSION   DAOR01000068 DAOR01000000
VERSION     DAOR01000068.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_19818"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2254 a         1463 c         1389 g         2566 t
ORIGIN      
        1 catgtgtgga agcagcacac ctagtaaaag gagtggttaa atttctccat tggaattcca
       61 atgcttaatt tcgaatatta tgacatctgt tctccacagt gtcatcactg ctatgaatga
      121 gtgaaacaat aactaataac tggagacgaa aaaatatata attcactcgc tcattattgt
      181 atatcctcat aattatttat cttgttattt caacagaatt aatcatctgt tgtacaattg
      241 gtaaatagtc gaagtatttt ggttcttcag caatatatct taagcaatca ggaggactca
      301 aatcatagag tgcttctata tgagtaagca tagtgctgta gatattattt tacattcttt
      361 atacatctgt cgcagtattt ttcaataaca gttgcacttt catctaccct gaacaaggct
      421 tcttgtgcag ctagggtacc gcagtcggca cagaattttg gcttgactcc tgattgaatg
      481 aacggtctca cagatgttaa cacttttttc atcttatctt cacatactta gaaggaagta
      541 gcctataaaa aatatgggta tttgatatag gactgcgata ggggtgaatc atttgattca
      601 tggccttaaa ttcccgcgtc acgtcgattc tccagtcgtt gtttgtatgc tttctacatc
      661 gcttgctgca tctctaactc cagctcataa gcgtcatgta ttccaaatcg gctgaagtac
      721 tccatctttt gttttttgta tcgtcttttt tgtcatcgct accacctggg ttttcaaata
      781 ttagcggatg tttaccgtcc tgtgtatcga aaatatgatg ggccacattc atagacgcag
      841 agaagactcg ttgccagtat tctttcgtat ccggttttat agcaagacga ttattatcaa
      901 gagcatcttc ccgtaaaaat gaatagtggt tgaatgttga tagtcattca caagcttgtc
      961 aagtctgtct tggttggcac ccatcttgtt tagcagctct tcatatccaa acgatgtatt
     1021 tccacctcct agtactgaca ttagtttgtt tattgaagca tactcgatga agtggcttct
     1081 cgcaaccggg atgtcatttg gatgaagcat tctggataac tccagatcga gaggagatac
     1141 aatcccaaac cctgtttcta ttccttgtaa tacacacctc ttttcattaa gcaattcact
     1201 cacgctccat acaagttgac caacctgcaa gtgttttgtt tttgaacaag ttgctagaat
     1261 tgcaagtgga tggattagat gctacttcct gattaaggaa cacagtcgtg agcaataata
     1321 gagaagcgtg aagacagaaa tagagatgga taacttgatc aagtagggtg ttgcgtacta
     1381 tagataaccc ctaaaagcct atttttcaaa gtatagaaga aaccaaagca ccatagcagg
     1441 aacagcgaaa cctgaggtat tggtagtcga tgctgaagtt gatatcgctc ttactttcag
     1501 aaagggtttt ggagtcagaa cgctactccg cctgcggtac tttttgaagt cctctcattt
     1561 gattttgccc agtggataca tacaaagcgg agcagaagtc ctacttagtg tcataagcct
     1621 ccttccattt tggctgcagg tttagatata gcctatttat ataacggaac tcatcttttc
     1681 ttagtctttg acagctcccc aagactcaca agcaaaaaaa actgagatat tatacggagt
     1741 tgaaaatact attagtatgg gagtgaaaaa tgcaaagaaa tggatggatc ttttcggtga
     1801 taaaaatggg ccatccataa ttattgaatt tccagagatt tacaaaaata aaaaagtggt
     1861 ccagcggccc tagtggatgt tattctttct acctttatgc tgctctctta tctgcaaatt
     1921 cccttgaatt gttttcaaac gttcttgcgg tattagcagc tattctcgaa aattctttta
     1981 catcatcctt ctctcgctga acaaatgctt tgtaggcacc gatatttgca aacattgtat
     2041 tgtttgctat tctacttgta gccatcacgt tatctgcaac attgcttact gttctcgcat
     2101 agatttcagc tgcccttcgt ggagatgccc aattattcca aaatgcgtca tacatattct
     2161 caatatatgg aacccacgtt gagtgaaatg attgtataat ctccttttga gattctagat
     2221 agctatctgc aatttctctg gatgcttgca gagtatgttc ttgataatca ttgattgctt
     2281 gtgtgttacg tggaatctct ctcctagctt catctactgt tctcctgata ttttctcttg
     2341 tttcatctaa tgctctttcg actgcgtgat tttgctccga taaacctgtt cttaattctc
     2401 ttttatcatc atattctgcg gaggatcttt ctgcttcctt ttttgttgac atacttatca
     2461 ttgccctcga tgctatttat agtttggtat tgaatggtgt aaaataccat taaaagccaa
     2521 acaattgaag aatttcgatt gagaatcgta acgggaacaa taatagatct tttcgcggca
     2581 gacttgctga attggctgat gctgcaattc cgctgagcat tattgagaac gcaaaaaaag
     2641 gtgagagtgt gtaattaacc agtactgttc agcatcagtg tctttccaac tgtatctccg
     2701 ttatcatcac tgcctttgaa tactggcata agaagagtcc tggtataatc aaaaggattt
     2761 acaaagacct gagagagatt tatcggaaat ttgtaataaa gcattatgca tgcatgtaac
     2821 tcaattcaaa aacattttgc atcattttat atcatcattt tgcttgctga aatatctgat
     2881 cacgactttg actactttca cttcgccggt tacaacgcca ttgctggtag ggacatacac
     2941 cgttatcgac acgactttgt ttccattatt aagtttgtcc acaatgtaag tgcatttgaa
     3001 aatctgtcct gaagtttctt ggcataagag ggtggcaaag ccatcaaact caaagcaagc
     3061 gattgagtta tttgatcgga aatagaattc ctagtatgtt attgctcttt tcaggcggct
     3121 tacgagtcta gagagttgac caactaccta cctactgata ctttatatga catattatat
     3181 ttctctctat ataattaatt aattgattga ttttgatgac gacatctata cgtaagataa
     3241 tgatgaaaat ggaacaaact ctctcttaca ataaactagc tagactcact gcactcaaag
     3301 gattacaagc cgaggagcaa caggatccct tcaaaatact tattggaact attctatcag
     3361 ccaggacaag ggatgaaatt acaacaaaag ttgttaacaa cttatttaga aaattcaaaa
     3421 atgcaagaga acttgctaaa gcagatatag aagaaattaa agcaataatt cacagcattg
     3481 gtttttacaa tatcaaagct aagcggataa aggacgtttc gcaattaata gtcgaacgct
     3541 ttaatggaga agttccagac gatatccgga agcttctcga attaccaggt gtcgggagga
     3601 agacagcaaa ctgtgtttta gtatatgggt ttaagaaggc tgcaatccct gttgatattc
     3661 atgttcacag gatttcaaac agattaggac ttgttaatac tacaactcca gaaaaaactg
     3721 aaattgagct tagtcgaatt cttgctaaga aatattggtt aaaagtgaat gatacttttg
     3781 ttatgtatgg tcagaatatt tgccttccga taaaaccaaa ttgtaaacta tgtaaattaa
     3841 aggaggtttg caaattttac cacactacag ttcttcctat ccagaagaaa tcttcttttt
     3901 ttaaaacaaa caatccatag ataagccgac ctaagccgca gtggcggttg ggtacgcttt
     3961 gacggcaatc gcggggggag tggcagaaac caattggttg ctaaaggccg catttattca
     4021 agtgaatagc atcggccttg tgattgctct cgcaagaatt cgtatgacta aacaattttg
     4081 aaagaaaaat tgaatatgca gaggcttcta ggccactaac tcatgtctca gccttcctga
     4141 ggaacactct aaaaatggga tattgtccac gtgttgacat cttttcagaa attccatatt
     4201 gatatcgatt caaaacctct cctaacttga cgatatccta ttttcaggga tctctacaac
     4261 tgctgttttc ttgaatttag tatgtcctat tcctttagtc aataatctct gcatcaaacc
     4321 gagtctttga gattgcttag tgacttgatc tgtttttttt gtttaaatca tctacttttg
     4381 agcgaatgag ggcaatttcc gttgctcttc gatggcagta agaggaatat catagggact
     4441 actattcggt tgattagctg ttcataatct tatatcattt atctatctct ttcttgtcaa
     4501 gtcagtaagt ttgccccaac tatttctgtg ttgagagcgc attgagcact aacgacacaa
     4561 accagctgtt ttaaaaaaag ccattcgaaa ttcataaagc ttagtttcac gaatagaaca
     4621 taattgtcat tattaaggaa gaagtatgag cataaattat ttcaatactc tttttttagg
     4681 ctttcggtat aactttgcac cccatcgaag aaagaaaaag aggatagaat ataagcttgt
     4741 actaggtaaa ttacgtttcc ctcacttcgg ggactgataa agtggatcct ttgttctttg
     4801 gacctggtga atctaaagcg cagggtgtag atgcatatcc aatgtagatg acctagaaac
     4861 gattagtcaa aaagtatgcg aacgtgatga tatgtatata ttcaatcgca agtgatgata
     4921 tgtatgagag agacacagcg gaagtcgtgc aagagacaga gataatgaag aatcaaagat
     4981 gataaacagt tgtatgctta gcatttctgc tatcgtcatt ttaccaaaaa tgagcttaag
     5041 aagaacgaat tatctactgt ttcaccttct catattcttc ttgtttagtt caatgagtgc
     5101 ctcgtagctt ctacaaaatt cgtcaccaaa tcccttcaat cttgtcatga accttatctt
     5161 ttgatagtag agatggagtt atagaatata ttgaaactca ggatgataag gctgctctgc
     5221 tatatctagc aaaaaaagct tttttagtag actacaccga cagccttttt catttccttc
     5281 gacagctcac gatagccatc tttatctata atgacggcat tgatgccatc accagtacct
     5341 gaatttcttc tgatggctgc tgcaattgct tggattgcta ttttgtaagc atcatttaca
     5401 gttagattat ctttgaattc cgtttcaagg taaccatatg ccaccggtga cccactacct
     5461 gtagatatga acttcccact tgtcattgaa ccaaagagat caatattgta tatttgtcct
     5521 tcctccttcg tcttatcaac tccggcaagg ataatctgga catagtatgg aaagtatcgc
     5581 tgattgaata atacattgga gcacaatcta gcagcagact tgattggtat aggatcttta
     5641 tttgtaagac ggtagatgtt tgcattatac cgcatgatat caactagatt ttgggcatct
     5701 gctactccac ccgccatagt cataccaatg tgtctatcaa ctttctgaat tttcataaca
     5761 tgtttatcag caataaagaa gccagcactt gcccttgtat cagcagccag gacgactcca
     5821 tctttacaag ttaaagcaca agtagttgtt cccttcttaa tatgctccgc tatgtactct
     5881 tcgtaccgat tgtccttgcc ttgctgatac ataatatatg attatcagaa tgataatcgg
     5941 ccagatttaa gcattatcgt ctttcatcat ctatgggacc cgctacatcg tgcctatctt
     6001 cgttcttttt actataatcc atttttctgg cttcttctac aattcgataa tagtaattag
     6061 gtgcttttga atctatttta gtgtctttct cttttttctc taattctgaa cccaatcgtt
     6121 caactgtttc tttgtcggat gcaattgcac caattgtata ctggtaatat cctgttatga
     6181 cctcactata caaaataacg ccttttttcc ttgattaact aattttactg catctttgaa
     6241 cgctgaacca aattcataat tttctcgagt atcacgcaaa ttattttcat atacatgcgc
     6301 agcaaatatg tcgccgaatt cactatggct taaactcact agagcaatat cggttccttg
     6361 gtgattgaag gcggtagaaa gggcacctgc tttaactgcg tctcttgcta aaagtcgtaa
     6421 acagatttct ttggttagtc atattggttg agggttaaaa tctcctaggt atgttcgatt
     6481 caattttgag tttgcttcta ctttgatgcc tatattacat aatttgaaat caggcccact
     6541 tgcagtgtta aatgtcaatg acgggtacaa tctatgcaaa atatagccaa tttggaatta
     6601 atcgttactg gagactatcg ctttcagaat ttggacagcg taatcggctt gcttctctgg
     6661 atatccgacc gcgttagata caagagagct caattgttgt tttagatcgt ttattcgctc
     6721 ctttccacca accatcaatt cacaatattt aatcatgtcg atcattttga taatgtgaac
     6781 aacataggga atcggaagct cagtttgttc agtttgaaat cgtgatgttt ctattgccaa
     6841 acacatgctt gaaaggttga ttagtggaaa gtcatatttt tccagacttt gccaataatc
     6901 tattatctca tctgttaaat gaatgcagcc aatttgtttg aggggaatca aaaaatcatg
     6961 tataatcaat gtttcattat cgaatttttt ttggtcggaa tgcgtctatt gattggtcca
     7021 tatcaaattg tacgaatttt gataacttta gttgaccaag aatattaaag ccacccataa
     7081 aagtattgat cttcatatga tgccgtggga gagattgtag atatttatct caaagaacaa
     7141 gaatcttata attgaattag acgagcaata cgtgttgatg ttcaaaattg tagcttcccg
     7201 atatctatct ttgcaagtaa ttgttggaac ttatattcca tctcttgacg tatggccttt
     7261 aattcttcct catgtttatc tttcatcatc tgaatttcat ttgctctaac ttgtaattga
     7321 aattcaactg ttttgatctt cgaaatgtga gattctaact tatttacctg ctttcttaat
     7381 ttctcctcat tttttattgt taagaactcc acctttcaaa taatcatcca gcagttcaat
     7441 ttctgtagct cgataatagg agtctgatat tcctattgag tgattcatta atatctcaat
     7501 attgactggc ttcatactcg aaatttcgca tcgtgttttg aatcacttac gatcgcatcg
     7561 tgttacggta gtagaattat tggagaaaag cgacaacctt gtcaaggtgc gaggacttca
     7621 tgcaataaat ggtactccta tactagacac tagttcgagt agctcgccag aa
//
LOCUS       DAOR01000069           20291 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_40239, whole genome shotgun sequence.
ACCESSION   DAOR01000069 DAOR01000000
VERSION     DAOR01000069.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 20291)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20291)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20291
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_40239"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         6187 a         3932 c         4425 g         5747 t
ORIGIN      
        1 gctaatgaag caatggacga ttatgagaaa tatagacctg actttgtcat aatcgattat
       61 aaactgcctg gaaaaaagaa tggattagag gccgcaaaag aaattctgat gagttaccca
      121 tctggcagaa tcttaatgct aacagcgttt catggcgtaa aggacgaatt gaaaaaggac
      181 tttttcttcg acgacaaaat gatacaaatt ctgataaaac ctgtgaaact tgcgcgacta
      241 gccgatatta ttattcgtaa ttaagcatca ttgtgctcta tttttcactc atgtgacacc
      301 gatctaattt taatcccggt tatgatagta tatatcataa cagatgttta tcgtaaagaa
      361 gctaagcaag aacggcgtct ggaatgccat tagtcttata gatcaaaacg ggtcgttcag
      421 aggcgaagca aagttcgatt ctaagaaaga ggcattagat taccttcttg aatacaagcg
      481 aagaatgaaa caccaacaac aggacctcga agtcttttct gaacccttgt aatagtgttg
      541 tcgtatatct atatctacgc tgcttgttgt tttcattacc aaaatggatc tcctgtcgac
      601 ggagagatta gatcattaag taggccataa ttatgcgata accttttacg gcataatgtc
      661 gtatagttca ttatgtatgc gaatttctgt tacctgtgta acaccgaaaa gcttacgagg
      721 ccatgttcga attgtggcaa gcctacatgc aaagactgta tgaagagctt tcccttctct
      781 actagcgtct gtttagagtg tgcatccggc gtcaaaacca ggctggccgg tattagaggt
      841 gacgaagaga ttttctaatc tttactttat tgttattttc atcatttggt tgtctttaga
      901 cggcgccctt ccttcttgat taatcataca tcctacaaca caacaactct ccttgttgtt
      961 cattcggatg gcgtgcctgc ttgttcaata atcttaatca ataatcatgt tcaaaatatc
     1021 tatgtcttta atacaattat ttaagtaccg gtttttacca aaatatcatg tagattatac
     1081 gattgacaac agcaaactgt tcgggatcat atctgttgat gagtatcaga tacaacagac
     1141 acaacttggt aaaaaaattc gaccctgtcg acaccaaaac ctctgattct tttaattcta
     1201 ccttctttcg tcagacgtgt aatggcgtat cggacttcat atctacttcc aatttcccgc
     1261 tcaagctgct tgcccgtaac tttgccgcta ttgtttcgga caatagagta aactaattct
     1321 gtgcagtcgt acttggaatt tttaccgagc tgaatcgaag tcatctaatt taccagtaga
     1381 tttagcctta aaagtaatcg aggctaactt tttttacttc ttgtgaagaa gcgtgcacag
     1441 acggacacat atgctctcat tatcaaaaca agtctatagt atttctagag atgcccgcag
     1501 atagcagaat aatattggtt aaattaattc tttattaact aaagtgggat tcagaaatcg
     1561 tttgatccca tcagcaatct tgaagagttc ttcgtgtgaa gccacacgtc tcatcggcca
     1621 tcggccgtta gggctgtcat cattgaacct cgggataata tgaacatgta catgtgggac
     1681 aatttgattt gccgctttgc cattattttg acccacattg aagccatctg cttttacagt
     1741 tggtacaatc gccctggcta taatgggcac gagtgaatat aatcttccga cttccctctc
     1801 tggcattaag agcaaattat cgtagtggtt ttttggaata atcagagtgt gtcctctact
     1861 tattggatac ttgtccatca ataccaaaaa attgtcatct tcgtagacgt taacaaaagg
     1921 caatctcttt ttaactatgt cgcagaagat acacgaactc ttgcccaatt cagtctctag
     1981 ccgtactaaa cttttaatat atttatagtt tagacacaaa atccaaacac tcttttcaag
     2041 tccatttttt tgcttgacta tatgttcagt acactcgaaa atacaatttg agcccttaaa
     2101 taatagtctg cgatccttac ccaaaatgat aagaacttgg ctgcgggcta taagattaag
     2161 atttctatca gcatctgtga tcgcagtcag caatggtata gcacttgctt tctggaaaaa
     2221 taaacagatt gacttatcag acgcgattct aacatttgct ggagttattt gccttcacgc
     2281 aagtatagac ttgttgaatg actattggga ttacaagcga ggtatagaca cagcaacacg
     2341 gaggacaaag tttagtggtg gaacaggagt actaccagaa aaacttttaa aaccgggtaa
     2401 cgtttattcc gttggcctta tttttctcct aatcgggact ctaataggga tgtactttgt
     2461 ggtaataaga ggcattatga ttgctttgct ccttggattc gctatattag cgatatattt
     2521 ttattcaaca agtatcgtga acctcggctt aggggaggct tttgttgcaa tcaaaggcac
     2581 tcttattgta gtcggatctt actatgtcca aacgtcgtcg atcggagttg gtgtggtcta
     2641 tgtcggtatt ataattggca tcttatcggc atcggtctta tttgtaaatt cctttcccga
     2701 ctatcaagct gaccgttcac gaggaagacg aactttagtt atattgttgg gaaggcagag
     2761 agctgtaaaa attttcccat ggatgattct ctgtacctat gttctaatca tagcgggaat
     2821 atttctgggt tacttaaaaa tatattctct tgcctccctt ctatcagcgc catttgctgt
     2881 aagtgcaatt aaacggatca gcaaatccta ccaagatgtt gagaaattag taccggtcat
     2941 gggtgcaact gtggcttact ctcgagtcac gggtatagtt ttggcagcaa gtttgtttat
     3001 ctaaatcatg cacaaagtta gagtcaacag tagtgaatct aggcccattg ttgatgattt
     3061 ctttacctgt ttttcgctat tccccaagct atcacgactc ctataacaca gaaggcaccg
     3121 acaagccacc atatttgtac atgtttgact atatcagcaa gagatccagc taacacgtta
     3181 aacagatcac tacgaagact aaattactgt aggtcaaaac attttgactg aatattctga
     3241 acaaaaagtt tagagcatag ttgacagact tgatgatatt tccctctaat cctaccggat
     3301 tggtattccc taaaagttca aataaattgc attcgtgttt cgaaggttta atctaatgct
     3361 gcttttccaa gtcgattgtg aactcagaag gattaagcgc attcccacat atcctacatc
     3421 gaccattagt aggtcgcaaa acatccttta ttgatttcaa acttattatt ttgttgatga
     3481 cggatccgca ttttccacaa acaatctcta tagatggata agacaagata aataatatcg
     3541 tatatatcat ttgatagaat atcaaatttt acgtcaagat ggattgacca aatcagtaat
     3601 aatagttttt aacggctaag tacagtgaaa ttattttgat acgtctgttg tacaaaatct
     3661 tcttatggtt tgttaccaga ttacgaaaaa ctcacttcgc ggatatacaa ggctcatgat
     3721 aataaataat atatctgaaa gagaccaata acgctgataa ggatgtgcat agtctaaaga
     3781 ataaataaat acactagtaa actcccataa ctgggatgcc tgtcgatcca gtttgcggaa
     3841 tagaaatgga tgaaaaactt gcgacaacct ataaacaccg gggtaaggac tactatttct
     3901 gctctgaagg ctgcaaaaag atctttagta gaaagccggg caaatatact tgaccagcgg
     3961 aaaatattgt ctccttttcc tatgaatcaa actattttca atgtgttatg actgttgttg
     4021 tgatcagtcc tgagatcaag atataccaag tttttgcatc gtgtgtccat ttttggttat
     4081 tcgtccttgc cttaaaaata actgatgcat gattatttca tctggccgct actgtttccg
     4141 cgaaatcaga gtcttgtttt ctagccaata gagaatgtac atatctcgtg gcgagaactt
     4201 tttcggagca tgcaatgcgt tactaaaaat ttataaccaa tgaatacatt gtattcgaca
     4261 taacaactaa ttgaccataa acaatgaaac ttaagggaat gattggaatt caaaatagac
     4321 cgtcaatgga taacgccttt gaaggcaaga atggaatact aggcttaata gatcctcccg
     4381 cggtattgta tggtactact gagattggca ataaccaaaa tattgcttac gaatttacgc
     4441 ctaaaagtat caagattacc attgtttgtg atggatcccg agtacagaat taggatgcac
     4501 tggtcccgta ggcatgaagt gcctgttaca caaggcatgc tagagctcat ttgagggtta
     4561 atgaacaacg ttgaaaagaa tattgatagt agaagatgaa tccgatatta tttttacatt
     4621 gaaaataatt ttagagcaaa atggcttcgt agttagttca ttcaccgacc ctctgttggc
     4681 attatcgagt tttaaacctt atctgcatga cattttgctt gtcgatatta aattgccgag
     4741 catgagcggc ttagagctgt ataaggagat aagaaagatc gatgaaaagg ttaagatttg
     4801 ttttctaacg gccagcgaaa taaattatga agagcttaga aaggcagtct ttccttcaat
     4861 ggaggatgcc aaatgcttta ttagaaaacc agttgagaat gaggctttgc taatgcaatt
     4921 aaataacatg ctagaataat atgttcatag actgcttctg gaccttgacc tcgacctgct
     4981 tctcacagag tctgataact aagtagttga acaagcgtta tacatataat ccaagcgtcc
     5041 catggataac ggaaaccggc caaactagga ataaccccat ttttatagct gataagtaaa
     5101 taggaagact taatgaaagc tgttttcgta agaggcaaga acaaggtggc cgttgacgat
     5161 atctcagttc caaatcttgg cgatcgagat gttctagtaa aaatgagagc ctgtgggcta
     5221 tgcggttctg atttggagaa agtttacggt gattacggaa tgtcgtctag cagattgggc
     5281 catgaaccta ctggagatat aataaatgtg ggaaaatcag tcaaaggctt cagcataggg
     5341 gaccgtgtat ttgtacatca tcatgtcagc tgccatgcat gtcattattg ttggcacggt
     5401 gatttcacaa tgtgtaatat gtatcaaaag agtaatatcg aaccatgcgg actatcggaa
     5461 cagatcttag tacccgaagc gaatgttatc agaggtggac ttgtgaaatt gccagaaaat
     5521 gtgacgtttg atgaagcttc tctcatagaa ccgttagcat gttgcataag aggtctggcc
     5581 aaatgtagac tccaaaaagg agacgatatg gcaatattag gggccggacc agccgggcta
     5641 atgcatgcga tcctcgcaaa attgaccggt gcaggaaaaa ttgtggcgtt cgatctcaac
     5701 cagttcagat tggattttgc caaagggcat tttggcatcg atacctataa tgttgccagg
     5761 gaaaaggata gcattaaaaa aatgcaggat attacggact gtagaggtgc tgatactaca
     5821 atcgtggcca caggaaattc aagagcgatt cttcaatcat ttgatttgac acgaaaggga
     5881 ggtaacgtcc tcctcttcgg ggttccaacc gaagggacta gggttttatg caatataagt
     5941 aaggtatatt cgagtgagct atcgattatt ccaagttacg catcatcaga aatcgaaata
     6001 aaccaagcaa tccgacttat ttcttcaaag aggattgaga taggttcgct gataactcat
     6061 cgttttgata ttgcaaacgc aggcgaggga atagagtgcg ctcatagagc agaagatgcg
     6121 atcaaagtaa ttattacagc gaagtagatc tgtggaaact ataaataatt acgacgtggg
     6181 ggattcagtg cagcaataag taatatggat tggggaatga aaaacagaat gtcaaaaatc
     6241 attcgtccgc aagatggacg aactgttatg cttgcagtcg atcatgggta cttcttggga
     6301 ccaacagaga gactagagaa tcctagtaga acgataaagc cacttttacc ctatgcagac
     6361 tcgttgatgc tcaccagggg cgttctccga acatcagtaa atccaagtac ggatgttcct
     6421 attgtattgc gggtttcagg aggaagtagt atcgttgggg aagatctttc aaaggaaagt
     6481 attactacat cggttgaaga tgccatgaaa cttaatgcct cttgcctcgc tctttctatc
     6541 tttgttggca gcaagtatga gcatcaaaca ttgaccaatt tagcaaaact tgttgatgaa
     6601 ggtgaaagat tcggaatccc tattcttgct gttactgccg ttggtaaaga aatggccaga
     6661 gatcacagat atctagcact tgcgtgcaga atcgctgccg aattgggagc acatctaata
     6721 aagacatact attgcgaaaa ctttcaaaag gtcgtcgagg gttgccctgt acctgtcatt
     6781 attgcaggag gcaagaaact gcccaacgaa ctcgacgcct tacagctagc gtatgatgca
     6841 gtagacaacg gtgcaatggg agtggatatg ggcagaaata tttggcaatc ggactatcct
     6901 gtggctatga ttaaagcagt cagggcgatt atacacgaga attcaaacgt aaaagaagct
     6961 aatgatatat tcataaaaga aaaagaactt gaacagcaga aggtaatcaa gaccgcatag
     7021 caaagcgccc tcgctttttt gccggtctct ctcttctcta caatctcctt tatcagtacc
     7081 tgtggtgtgg tgtgttttcg gcgattcaga tattatgagc gtcttccaga cgcaggtgac
     7141 aactacaagt aatgggaatg gccaactttt tgagaaacca gagtacaggg accaaaaaat
     7201 gcttgattgc atatggagtg tactaaattt gcactttagt ctggacacaa tctttataaa
     7261 ttgtgttgca gcaagcagtc tgtcgaaaat gcctgatgct aacggtgaag agtatactgt
     7321 agattcttcg caactacaag taaatgttcc aagcgcagcg gttgtcatgc tgctatagat
     7381 acagctctgc acattgtttt agcagggcga tgtttgaaaa agaaataggt gaactgtgta
     7441 aatggtagaa atgatcggcg caaaagcttt gatccatgct ctcgaaaagg agggggtgga
     7501 catagtgttt ggactgccag gtggggcgaa cctgccaatt tacgacgctc tcgttgatgc
     7561 caacttcaga catatacttg tcagacacga acaatcggca tctcatatgg cggatggcta
     7621 tgcgagaata aagcgaaagg caggggtatg ttttgcaact tcaggtcccg gcgctacaaa
     7681 tcttattaca ggtattgcta ctgcacatgc tgactcttct ccaatgatag ctgtaacagg
     7741 acaagtgccg ctggcaatga ttggcaagga tgccttccaa gagacagata ttattggtgt
     7801 tgccaatccc tgcacaaaat atgccttcca accccggttt gcacatgaaa tcccagagac
     7861 ggttaaaaaa gcgttttata tagctgaaag tggaaggcca ggaccagtac tgatagatat
     7921 cccgaaggac gtacagcagt caagcgctga tatgaacttc ccagaactta taaaagtaag
     7981 agggtacagt ccggtggtag atgcagacct ttctcaatta ggtaagtcag tggaaatttt
     8041 gcttaatgct gagagaccca taataatggg tggagggggt gtcattctct cgggggcttt
     8101 ttctgaattg caagcagttg cagaaatact gatggctcca gtggtgacta catttaaggg
     8161 taaaggatcg tttcctgaaa atcatcctct tgctatgggt ccaattggga tgcacggaca
     8221 cgcagaggcc aacaaaatga ttttggaggc agattgtata ttggcagtgg gcgcaagatt
     8281 ttcagacaga tcggttggaa ggtacgatga atttggaagg ggtatgagca tcatacatat
     8341 ggatgtcgat cctgcggaaa ttggcaagaa caaatcagta gatgtagcag ttatcggaga
     8401 tgtaaagtcg tctctgagaa cccttgtcaa aatgctctca aaggttatag taaaaaaaga
     8461 tcaagataac ccgtggctga ggagaagaaa agaacttatc gattattatg cagagacact
     8521 aaaggattat ccaagagatc tgacagccag aaaagcgtta aaaaaactta gggaaatatt
     8581 gccggctaat tcgattgcga ccactgaagt gggccaatgt cagatgtggg catcgttaca
     8641 ctttgacgtt atagctcctg gcactttttt tagctcaacc ggacttggga ctatgggctt
     8701 cggctttcct gcttcgatag gtgcaaaggc tgctaggcca gtagtacctg tggtcgatat
     8761 agctggcgat gggtcattta acatgacgga gaactctctt gccgtgtcgg tgctggataa
     8821 cttacctgta attgtctttt taatgaataa ttatatgctt gggatggtgg ctcagtggca
     8881 gagaacgttt tataacagac gctatatggg ggtccaccag cgtaactgcc cagattatgt
     8941 aaaaatagca caagcgtatg gcgcacaggg tattaaagct cagtctttgt cagagttgga
     9001 aaaagcaatt agatcagctc tcaagatgga cgttgctacc gtcattgata ttatcatcga
     9061 ccctgaagaa gatgtttatc cttttgtaat accaggtaca ggtctcaaag acatggttac
     9121 aggatagtga aatagttgtg gtaacaacaa ctttacccgg caccccggca gcaccaactc
     9181 caaccactgc cttaaatgaa aaaatcggcg gctggcatat attgtccgct ctgctggaaa
     9241 ataaacccgg cgttctgttt agggtgacaa atctctttcg ggcccggaat tttaacattg
     9301 aaagtataac tgtaggttcc accgaacaag ccgatctctc gaggatgaca attacgctta
     9361 atagtgacga aagaacgctt gatcaactcg cgaagcagct taggaagtta attgatgtgg
     9421 tagaagtcaa ggtacttgac acagataaaa cagtttatcg tgagcttgcc ttgataaaaa
     9481 tgaatgcagg agaccccact acgagaatgg aaataaccaa ttttgcatct atttttagag
     9541 caaacatact cgatatcgga aaagagacaa ttaccgtgga gcttaccgga acgcctgaca
     9601 aaatcgaggc ctttaagaat cttgtcgatc actttggaat cacacagctg gccagaactg
     9661 gtgtttctgc tctcccgagg ggtgcagaac tttagatgtt aggcgctgac agtaaagtaa
     9721 ggatatttga tacaacgtta agagacggtg aacaaacacc tggagtttca ataaccccag
     9781 agcagaaagt acagattgcg attaaacttg atgagcttgg aattgatgct attgaggtcg
     9841 gctttcctgt cgtgtcccac ggagaaacaa ttgcaatcaa aagtataacc aaacaaggat
     9901 taaaagcaga aatttgtggc ctggcccgcg ctataaaatc agatttagat gctgctataa
     9961 attgcgactt aacctacgtc cacactttta ttgcaacatc agatattcat atgcagttta
    10021 agttaaaaat ggatcgacaa caggtgctgg aaaaggctgt atgggcggta gaatacgcca
    10081 aaaaacatgg cgttgaggta gagttttctg ctgaggacgc aacacgatcc gatcgagcat
    10141 tcttaaatca agttttcatg gccgtggcga atgtcggtgt tgatagatta gacattccgg
    10201 atacagtagg ctacgctact ccccaatata tcgaacagct tgtcaaagat gtaaaggcat
    10261 acactaggct tccgattagc atgcactgtc acgatgattt tggtctggcg gtagctaact
    10321 cattatcggg aataaacgcg ggtgcagatt gcgcccatgt gacaataaac ggcttagggg
    10381 agcgagcagg taatgcatcg cttgaggaat ttgttatggc acttcaatgc ctctacggta
    10441 ggaaacataa tgtcaaaact gagttgctat atgagacttc tagattcgta tcaaatacga
    10501 tgggaataat tgtacaacca aacaaagcaa taatcggtga aaacgccttt ggtcacgaat
    10561 ctggtattca tacccacggt attataaata atccgcttac ctacgaacca attagtcctg
    10621 atcttgtggg tagaaaaaga tggatgcaag caggtaaaca cgctggtgct cacggcataa
    10681 aggcaatgct ggaaggtttt ggcatcgggc cgacagagaa ggagctttat gagattgtgg
    10741 aaaagcaaaa gaaccttgct gacaagggga aatctatcac caccgcagag ctcctgtcta
    10801 ttgcgggcga ggtcatgcgt aataataaat tcgaagagaa attcaaactc tatgattttc
    10861 atattgttac agggatgaat ataataccga ccgctgtagt tagattaaat acggacggca
    10921 aggatttcat tgcctcggaa attggggttg ggcctgtcga tgctgctctg aaagcgatac
    10981 aaaagattgc aggagaaatg gctaatataa aaatccggga ataccggcta gattctatca
    11041 ctgggggctc ggacgcatta gctgaggtgt ccgtgaaggt ggaggacaaa aacggcaacg
    11101 ttgtttctgc aagaaaagct ggcgaggatg tcgtggttgc ttcagttcag gctatgatgg
    11161 atgcgattaa caaagttatg ttgcaaaaag tgctctattc aaagtattaa agtttatacc
    11221 tagttatata atttggttgt agattgggaa ggtataatgt tgcgctaata gtaggtgacg
    11281 gagtaggccc agagctcact tcgtctgcga aaacgatact tgaaacgata aacgataata
    11341 gtaacattaa atttaatata agaaatgtag acgcaggtga catggctcta gccaaatacg
    11401 gtaaagccct tccgcaattc agctctgatg taatacaaaa gtcacaagta tgtttaaaag
    11461 gacctgtggg agagagcgct gcagacgtga tcctcgtttt acgacggcag tacgatctgt
    11521 acgcaaatgt tagaccggca aagtcctatc ctaatattcc aaatctttca gccaatgtag
    11581 acctagtaat agtaagagaa aatactgagg acgtttactt aggatgggaa tttgacgctg
    11641 atgagaatac agtggtagga ctgagggtaa cgtccgaaag ggcgtctaga cgtattgctg
    11701 agtacggctt taaaacagcg atgtttcgga ataaaaagaa gagacttgta atagcacata
    11761 aggccaacgt cttgcgcaag ggtgacggat tatttgcaag aacctgtaag tcaatcgctc
    11821 aaaactatcc cgaaattatc tatagcgaat catatgtaga cacatgcgct atgaatctga
    11881 tccggaatcc agaagaattt gatgttattt tgacaacgaa cctttacggt gacatattgt
    11941 cagacgaggc cgcgcaggta gtaggagggc ttggattggg cccagctgct aatattgggg
    12001 agtcttttgc cttgttcgag ccggttcatg gagccgcatt cgatattgct ggaaaaaata
    12061 tagcaaatcc atcttccatt ttattatcta taaagatgat gctggagtgg ctatcagaca
    12121 aatatcatga taggggcgca tttaaagagg gacagcgtat agaatccgcc atggttgtgc
    12181 tgctaagaaa aaacctaaaa accaaagata tcggcggcaa actcacaact accgaattta
    12241 cgaagctact atctctttca atgtactaag gaggttcagt gtgacttgct tacgagactc
    12301 attctgtgga tgatcacagc attttggcgc tcgttgctcc caagcaatta atatgaagac
    12361 gaatggaata ttcataactg caggcgaaaa taagaccggc tgaaaagctg tgacaccgag
    12421 cttctaaccc atcatttgcg gaaaaggggt gagcaacggc gaaaaccaag attgagaact
    12481 ctttctcagc aactgagagg aagctaattt tggcgatcag acctaaaaca tttgtgagtc
    12541 gtagtattac cttgaaggtt tcaaaaagta gaaattttgt tttggcagaa gaaactttgc
    12601 tttcgcaagc ctcgatctgc aatgagcaaa tcggtaaggt cagagtttat ctatccatat
    12661 ataaatcaaa agtatgcatt aaaagacttg tatcttcagc ggtgtcatga aatgctgaag
    12721 tggaccaatg aataacacta tagtcactga ttttttggaa actagaggga cgaaaccggg
    12781 atagctcata tatagaacag tttcacactc gatcgctatg tctgccaaaa gatggctgga
    12841 tgcacaggtt aaaatcgatc caattaaaaa ccaatccgtc gcagtgatcg gctacggtat
    12901 tcaaggtcgt gcccaagcga gcaatatgaa agattccgga atcgacgtaa ttattggtct
    12961 gagaaaagat ggcaagacct ggaaattagc agagcaggac ggtcacaaag taatgggcat
    13021 accagaagcc gtagctggcg ccgatatcat ccatattttg attcccgaca tggaacaggc
    13081 agatacctac tccaaatata ttgctccata tatgacagaa ggaaatgcac ttagcttttc
    13141 tcacggggcg gcaattcact ggaaatggat agtccccccg aaaactgcgg atgtgatcat
    13201 ggtagcccct aaaggtccag gacaactggt gcgagaatta tatcaacaag gttttggcac
    13261 cccatctttg gtcgccgtgc atcaggatca tacagaaaaa gcttgggata gggtgttggc
    13321 aatggcgaaa ggtatcggta gcacgagacc aggagtaatt caaacttcct ttaaagagga
    13381 ggtcgaaacg gactggtttg gtgagcaggt agatctttgt gggggcgcac actctctggt
    13441 gaaggatgct tttgaaacgc tagtagacgc aggataccaa cctgaagttg cgtacttcga
    13501 atgtcttcat gaactaaaat taatagtaga tcttattcaa aaatatggga ttactggcat
    13561 gtataaacgt gtaagtgaaa ccgccagata tggtggattg accagagggc caagggtggt
    13621 taatgaggaa tcaagaaata gaatgaagga gattttaaaa gaaattcaat caggccagtt
    13681 cgcagaagag tgggttagtg tttacaagaa agagggaaaa aactcgttcg ctcgatacat
    13741 gaaagatctt gaaaatcacg aagtagaaaa ggttggaaaa gttatgcgca agatgatgtg
    13801 gccagattcg caggagaatt aatacctgct gctcaaatac aaatctattt gatgatcaag
    13861 tttggtgcac cactctaggg catacctttg attattccat tttctccgga tatccttata
    13921 aacaatccca attattagtg aaaagaatta tttcttgact cccaaagtcc ggtttttgag
    13981 cctcatgtgg cttccatgac attttctgca ccttgtggaa gagatgggat tctttacacc
    14041 gcatttaaag cagactttaa agaacagtct gcgctgttgc gctatctgtt tcttggtagc
    14101 atccgtaatc ggcaatattt tcctcggcta accatggaca aaaccttgat ttgtaccttt
    14161 ctattcgttt aacaagccaa agcgagtatc tgaatgccca tcattgccga gtctagataa
    14221 cagctcagcc tgtctcatct ttgcggtatg ctctgtgcct gtaatcaggt tatttacaaa
    14281 aacctttatt catttcaaga ttcgtatagt aaaaaatcat gttcgatcaa aaaacgggga
    14341 atagttttaa actttgggtc tatccctatc ctatgcacga ctattttgag atagatcgtc
    14401 tgatcgaaga tctcgccaaa atatattcta ctgcatgtgc taccacttgc tttaagctat
    14461 cgaaaaccaa gatgcccacg agggcagaat tccggaccaa agttgtagaa ttcatggaac
    14521 atttcgaata tacattgtcg acctttccgc aaggcccaga aatcgatcaa tttagagatt
    14581 acgctagggg gatccttcga aaggagattg aaaaggtgct tcaaggggaa aacaaagaag
    14641 ttgagcgacg ttacaagtat tatgtagagt acaattaggt gtgtacgttt tcttcgtaga
    14701 tttggggaga gggataggtc ggtctgcctt tacgcaaatg ctgtgtgagc tcatattaaa
    14761 tttaaattat caactcattt ggtgtcaggt tcgagccgct agcctaagag atcatgtgtc
    14821 acattattag aaaggtttta aacttgaggc aggagagttt cgatatgcga attacactat
    14881 cagcaattaa agcagacatc ggaggaatcg gtggtcatac tcgcccaagt gaagaactgg
    14941 ttgggacagt tagaagcttt gttggaagaa aggcaaaaga tctgttaatc gattattatg
    15001 taggttatac aggggacgat attcatatta tcatgagtca tactctcagc gtcgataacg
    15061 atagagtcca taaacttgct tgggatgcat ttacagaagg gactatagtt gctaaagaac
    15121 aagggttgta cggtgccggc caggacctcc tgaaggattc gttttcaggt aatgtgagag
    15181 gaatgggtcc aggggtagca gagatcgaga tggaggagcg gccaagtgaa gttttctgta
    15241 tctttgccgc agacaagacg gaacctggtg catttaactt tccgatgtgg cgtatatttg
    15301 tcgattcaag aagtaataca ggtctactta ttaatgaaaa gctggccgct ggagtaactt
    15361 tcaggataat ggatgtgatt ggtggtaaga tagcagacct aaaaatgtgg gaagacaagc
    15421 cagatctcga agcagcactt atgtatcctg gaagatatgt gatacaatca gttctttcat
    15481 ctgatggaga ccatattgta tcggcatcga ctgataggtt acacaacatt gcaggtacat
    15541 atgtggggaa ggatgatcca atagctatcg tgcggacaca aaaggatttt ccagcaaccg
    15601 aggagacagg cagtgcattt aacaatcctc actttgtcgc aggaaacaca aggggtagcc
    15661 atcatatgcc gctcatgcca gtcaaattga attcggcagc tagcttaaat tattccttgc
    15721 caattgtttc atgcatagta ttcagcatgc ataacgggaa aatgaccggt cctcttgacg
    15781 ggtttggcac cacagactgg gatctgcaac gaatgatggc agctgaaaga gcaataatca
    15841 tgcgtaacca aggattcatt caccctgcga cgctcgtgcc agaagaactg gagtacaata
    15901 agggatatga agcaagaatg tcaaaactca acaagaaatt caagcctcta gcaggagaat
    15961 caaaccagaa caaacacaac agcaaagtac aggaatagtg ggggcagttg aggcttcctc
    16021 ttattattat caattttaaa aattacccag aaacctatgg ccaaaacact attgagttgt
    16081 cgaaagtagc tcagaaagtt gccaacgata cgaaagtaga gattgttttg gctccgccgc
    16141 aagcatcaat cgctgctgta agtcagatcg tcccaatccc aattctgtgc caacatctcg
    16201 acgatgcgcc cgaaggtgcc gctacaggct tcttcattcc tgaaatggcc aagtctttcg
    16261 gagctagtgg ttcgctcctg aatcatagcg aacatagact agggagacaa accatccgta
    16321 agctaatcga acgtctccgc actctgcaga tgacttcggt agtttgtgcc aaaactcctg
    16381 acgaggtagc aaatattgca aagtttcgcc ctgactttat tgcgatcgaa cctcctgaat
    16441 taattggatc aggaaaagct gtttcgaaac ataatccctc actcattacg cgttcagtag
    16501 aagcaaaagc ccggtattct agttctacaa agctaatttg cggcgccgga attgtggaaa
    16561 aaatcgatgt cagcagcgca gctcgacttg gtgcagaggg gattctagta gcaagtggca
    16621 tagtaaaatc acactcttgg agcgagaaaa tctacgaatt ggcttccgga tttaacgacc
    16681 agtaatcaat cgactactgt tctagaacag aagcctaaga aagttccttt aagactaaac
    16741 tccaatttct ttcgctaatt tgataagctt cttgccatct cctgtgagtt tgttatatac
    16801 cttgttcttg acgacttttc tttggaccaa tccattacgt tgcgccttca ataaaagttt
    16861 atgaccataa ccatatgctc ctagtctctg gagtaattct cgagtcggat attccgaata
    16921 tcctatagtc tttaaaagtc tgacgatagc tctctctcta acgagcagcc gcaatagttg
    16981 tacctgtttc acaatatcat gaggataaag aaattattta atcgttacta atgattggca
    17041 acaatgtatt tttatttctt aaacatttta gtgtcacgat ctccgatcta gtctattaag
    17101 ggtgcagtgc agtagaccgc atatcatatt tgaatatttg caagccagct tctttttggc
    17161 agcaaagtat catcggtatt gtgagcaggt actttttcac ctcagacagg ctacgaaacc
    17221 tgatatgaaa aaagataagc tggtccaata ggttttttga tcaaaaactg tctaatcaat
    17281 aatttggaac gttgggaggt aatgtcaagc gtttatgcaa tcatggcctg gactctgttc
    17341 ttgtcggatc taatcctttg ctgatctcga gacaggccca gttcgcatct tagcctcaat
    17401 atctgttata agattggtca tgtcaggttt gtgcgagttt agatactcgt gccccttgtc
    17461 ctttaaccta atttgaaaga gtctcaaatc tctgtgttca tcaaagaact gctctatcat
    17521 tgcggttccg tgcttcgtag ggttaataag ctggtcaaaa cattcgatcg ttgcattaac
    17581 atcacctttt tctattgatt ctttcgcttt tccaaggacc tcgataatat cccttaactg
    17641 ttttacaggt gcagatactt ccttgctgtg accaaaaaaa ccaacgcgtt tagccactcc
    17701 tactttatcg gcgatctcct tgactaattc gtaatactct tctttacttg tgaaaaaacc
    17761 atggatttct ttactttctt ttcttacagt caatcctttc attgataacc gcataaaagc
    17821 gtcttcgacc tccgatttgt ccatgcctgt aacccagacg attttaccta catggttgta
    17881 gccttgtctt atcgactcta ggactacaac ttcaatgata cgtctaaagc cttcctctgc
    17941 tttcgcgttc tcaaagtctc tatcgcggac ggctcgcttt gcatatgata tatctatctc
    18001 tattgaacgc ttgagagctt ccatgccttc gtccaccttt ccgacaaccg tcagatagca
    18061 tgctttgtta taccatgaca ttccgtcgtt gggattaata tcaattgctt tttcgcacgc
    18121 ttcgactgct ttctcgtaat tattcattct ataatacgct aaccctttca aattccacgc
    18181 ttccccgtta ttgacatcta cgtctaacgc tttgtcgtac gaggaaatgg caacggggta
    18241 ttgagcaaga tgaaaatgcg cgtatccttt tttaatccac gagtctacat attttgggtc
    18301 aattcttaaa gccagctcaa aactgcgaat agcatcttct aacttttcgc tcgccatttg
    18361 gtttacgcct ttctgataca ggtcacgctt ttggaactcc gaattgtcat cgtaattcac
    18421 atgatttgtg gtttcagaag ctgggttaga ccttggctta ggcttccttc ccaggaattt
    18481 gcccatcaag atttattgct gttctcgctg ataaatagat ttggagtctg tttcattccc
    18541 caagcataat ctccgtatca ttttccagcc aactccgctt ggtgtttaga tttgccaaat
    18601 gcgactgcaa aacgattatt tagcagtaca gcgcctataa gtatactcgg ggttatctaa
    18661 gtgaaattaa atgatgctga cgtagaaggc ttgacaggaa aaagttttag aattataatc
    18721 aacaatggaa aagctggtat ccttcaatca caattatgga aggaacttga tctgactagt
    18781 cgtgatggtt cacgggtggc catccgtctt gaaaaaaggt ctcttataaa gcgtgagaaa
    18841 atattagaaa acggacgatg gacttataaa ctctttgccg tcaaattacc cgtcggcacc
    18901 caaactatcg aagaggctcc ctgcctgaca tgtcccgtcg aacatatgtg ttcagttgac
    18961 gctcactata gtcctaatag ttgtaaccta attgaaaatt ggattgtagt ttcttttgat
    19021 tctgcaaccc aacaagaaga acagcatcaa aaacaccctg tgcaagaaat tgtcccagaa
    19081 catcatgatg acccaattag aagtaacgta aatgaggctg agctattagt cacagagtcc
    19141 aaatcataga tggcaaaaca ccaatcgaac aaagctatta aaaattaaca agaaatgagg
    19201 aaatcgcagc acttatattt atctatttga ttactttgaa atttcttagt taattttgta
    19261 attctatata gatacttcca tctatttcta atgctggata tagagccaaa ttacctgatt
    19321 ttctccattc tgtgttttgt tccatccatc tctcgtcttt cttccatgac ttcatatgtt
    19381 ccaactttcc ggtgaagaga ttgaattcgt atccgtgcat gtaacaaaca atgttgtctc
    19441 cattaaagtc acccttggaa agagacgcac ccctatgagg acacgagttt ttgcacgcaa
    19501 ataatcttcc tcttccaagg ttactagtaa tttagtatat ggctacctag aaatcacttc
    19561 acctacgaag ggtcaacaga taccggcagg tagtatactt aacgtaacag gcacgcctac
    19621 accagccaac gctactgacc attgcgtagt atctgttatt ataaatggaa ttcgcccata
    19681 ccagaaagct ataccaacag gtagtggtga tggaactgaa gactatacct catggaagtt
    19741 tatagataat cctagttaca gtacaattaa gtcaggacag aataagataa cggtcaagta
    19801 ttcttgtatg cctaattctg attctagcaa tacgcaacct aactttgtaa aatatcatag
    19861 cgttaatgta acaggaatat gaggttagga gaatcaatgt taggatacca taggagagaa
    19921 ctggttaacc agtagtagat tttatggtta gtggcggcgg cattagcagc actaataaac
    19981 tagaagttgt tataaaggtg gctcttgtta ttcttgtagc gccaatattg tcatttataa
    20041 acgctcagtc ttcaatctat tcgccatctc acgaaagaga cttattcatg atacataaag
    20101 tgcctatgtt ggatagcagt agtaataaca gtagacaagt atcacatacg gttgacaaga
    20161 agcactaaag catggagtaa ggataccttg aatattgtat ctactaacaa tgacattaac
    20221 ttggacaaaa atcttagata ttacaatgtt gtggtgaata ggatgacaat attggctgat
    20281 tattttctag a
//
LOCUS       DAOR01000070            3362 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_34683, whole genome shotgun sequence.
ACCESSION   DAOR01000070 DAOR01000000
VERSION     DAOR01000070.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_34683"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1026..1231
                     /estimated_length=206
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          983 a          574 c          566 g         1031 t
ORIGIN      
        1 aaaaaaatga aaatacaaga agacagtaaa ctgggtgact ggtttaagga atggtataaa
       61 aacctacaga gtaaagctcc agactcagcc gaaagcatcg gctgggtact aagtgcgttg
      121 gatttgatga ataagatggt ggctaggata aagcaacttg aaggacagaa tggctagatt
      181 atctacaggt taattgtgga ttacctgtca cattgctgaa cacggtagct gcagatttaa
      241 gttacggcgt agtaaattac ctattcaaag tataaagtag tggctagatt caagacatat
      301 actgaaatct tgacggggtt aaaggctgtt gatgtcatca gttatcttac cccatttgaa
      361 gctactgcca cactgcttgc caccagtagg ttcatgtaat tgccgagaac aagcacaaga
      421 ctagaccaac tttaacatga tagaagagct actgagttag tttattcgca aactaagaaa
      481 cctattccaa cgagctatag attatcgttt gattggagtt agtcggattg gaaacgaggc
      541 gcaaggagtt tagaatacta aatccaaagt caacctagga ctatgaattc aaccatattc
      601 actattgcaa taagtgtaaa cattggtttg ttgtaaagca gtcaaaggcc agttgcaaac
      661 tgtcttcatc tgttaccagc cacgaagacc acgttgcatc tatcggtcag acgaaagcgc
      721 cgnnatctac aggttaattg tggattacgc tgctaacatc ttttattttc aatctgttga
      781 tacaaaaaaa gctattagta gaaaaatact ccagttcttt acacatgcga tagaatagca
      841 aggaacacta atccttccga tacgtcacat tattcgatac aatattattt tcttttaact
      901 taatcacgat tgtattgctt gccctgttaa atattctctg tctattgttg ttagtaaata
      961 tccatcctat aatgacatct agaggaagta agaatgcttt accgaaactt tctattacag
     1021 cagttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgggcaac tgatcagtaa ttccaagtat
     1261 taaataccat tgcctgcatt accgtgggtg ccaatagctt gatccagtct tttgtagacc
     1321 tgttctaatt acctatctgt ttgttagttt attctttttc tgttgatttt tgtggattag
     1381 tttattttat tatttgaaaa gaattaatca tttcctgtat aattggcaca ccaatgccac
     1441 ccacttcgta ggttagaata tatgccttat cgccctttat tgctccaatt tccatatctt
     1501 tgaaaatagt cttttgatct acataggtat atatcgattt gtatgcaggt aacccagcaa
     1561 gaacactatt tgtagttaat ccaaccaatt tgaaactctt aagagattgt tttaaatcac
     1621 taatgccact acttgcgtat tgagctaaag ctatgccttt ttcatctgaa atgtcatcta
     1681 ttgatatatc aagacttgca ttcgagttta tcgctggagg agggaaagta acaacgtcag
     1741 tttctgtgtc ctgtcttgta ccgttttctt atttatccca gctggaagga tattgtagtt
     1801 ttattacgta ggtggaattt acaaatggca agaaattcgt agttgatttt gtggtttgtt
     1861 gtgcaaacac tgcttcctta gttgctacag ttggatatac ataagatata atcacaatca
     1921 atactaacgt taataatatc gacatcaagc atgaaatctg tgttagcgat tgatatggca
     1981 ttctgcgctg cgaaatttct tagtcaagga aatctattat aaagcgctac atatactcca
     2041 tccacttttc atggcttcaa aattttcatg acaacatccc agggagtaac tatagaatta
     2101 tacagctctc tttcttcttg cttttcaaga tccttatgat tatgaggatt tacatgaggt
     2161 aagatttttg aacctttaag tatatgtatt atggaagata tttttgaata aattgaatta
     2221 tgaaaattat tattattcaa ccaatcttat ttcatatgac tgcagacctt tcatcttcgt
     2281 tagaaatgca tctatccaag gagtttgacg cctcagttaa aacagcacca ccaataaatg
     2341 aaactcctct actcaatttg tttaacaaaa atagaaaaca atggaaatca agcaatatac
     2401 tacaatggct tttggatagg aataaacccg atagaagcaa aaaaatacta gcagtcggtg
     2461 attttgatgc gtactcgaat agcctaaatt ttgtactagg gcagggccat gttgatggga
     2521 gagtatcagc tatttacctt ccaaggttaa gacaagatat gtagcataga tagggcatgt
     2581 atcctcgagt agcaattgtc tttagctaat ttctcagctg ccttctggat atgcttttct
     2641 ctatccaact cgttcatata atttaaactg aaagttatag ggattccgaa agaaaaaaag
     2701 tagcatagct tccggtttct gaagcaattc aatccaactt cgataaatat aagggagtta
     2761 ttaagatcgg tactgcagtg caatgcacag tactcctcga gctattatat ttcctgttta
     2821 aacgagaata cagccaatag gcataaccat cttgatcctg tctctttccc gagtgacagg
     2881 atcaatcata attaagagta ttttcaaatg taagcatatc gttattttac aactaataca
     2941 gggcaatggg cataagtcac tatgccagag gcagtacttc ctagcaataa tttcttaaag
     3001 cctgataagc ctcttgaacc tatgactatg aggtcaatgt tctgacgttc agcataatct
     3061 actattgctc ctattactga cgcagggtca acaataaatt ctatttttaa cttaacgccg
     3121 ttctggtctg ctttttcttt aacttcaatt agccattttt gagctttttg ttttgcgctg
     3181 tccactattg tatctatggt actagatgtg accaacgttg caacagttga tgagtataga
     3241 tatttcattt gtgaatgaat tacatgtaat acaattatct gaaagccatc actctttttt
     3301 gccattgcaa taccatattc tgtagctttc atagacgact ctgagccatc tgttggaagt
     3361 aa
//
LOCUS       DAOR01000071            3946 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_27487, whole genome shotgun sequence.
ACCESSION   DAOR01000071 DAOR01000000
VERSION     DAOR01000071.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_27487"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1224 a          644 c          819 g         1259 t
ORIGIN      
        1 cagcaatagc agtaataaga aattactctt tttagttctt agttttttcc ttgtagttaa
       61 cattgcttca agtggaggtc atctcgattg ggtcgacgga gtggaagcat ttcttgttac
      121 tgagagtatg gtattgaaac attctgcaaa gttatatcct gatgatccga gtatacagaa
      181 acttcattac gcaataggtt atttgatgcc tatcaataag gcgctgcaga ctggaaaacc
      241 ttacgatcca aatatacgga ttgaacccat ttatttgatt cgatcattat ttctatcggc
      301 aatagcagtt ccattttatt acgcagccat ttttttctca gtctctccca tttcctttgt
      361 agcaatcttt gtaaactcat tattgatctc attgatcagt gtgattatat tctttttttc
      421 tttggagatt tatcgttcta aaaagatagc tctcatatta agcctcatct ttggtgtttg
      481 ctcttttgtt tggccctatc atactacttt atggtcgcaa cctttgcagg cactctgtct
      541 tgttgcagca gcatatttca tttacctgtc attacatcgc cactcttcat ttatctgtca
      601 ctatatgaga gataatagca gtagtaatag taatagtaat acggttgttg atagttataa
      661 taactataag aaagatacta ctaaaggaat ttatcttgct ggactaggag gactatttct
      721 aggattatca gtatttgctc atcctaatag ccttattcta atccccggct ttatagtata
      781 ttctattgta tttatgaggc acaataaaag aactcttatt tcttttctga tggtagcagg
      841 tattgtttta cttttcatgg ggtttgttaa ctactggagg tttggttcat ttacgcaatt
      901 tggttatggt tattttgaat cattatcagt acacagcgga tggattggct tagtaggact
      961 gttggcaagc cctggagcag gaataatttt ctattttccg atagtaattt tgctccctct
     1021 atcattcaaa tacatgtata aagagaataa agggttattc tttttatcgg cctacattat
     1081 cctagcgacc tggttgtaca ttggtacgtt ctcgtattgg gagccctttg cttggagcgg
     1141 aggagtagca tggggaccaa ggtatctgat accagtattg cctttcatta ctctagtatc
     1201 tggagcaata ctagtaaatc tgagaaggat gaatgctaaa agaagactac ttttaaaact
     1261 ttcagtaata gtactatgta ttgctggctt ttctgttaat ttactcggca cgcttgtttg
     1321 gtttatgtat gatttagttt atgcgtggca aagagaagga ttaggggcca gtgagtggcc
     1381 tataataatg gcatggaata gccattattc gccgattatt ctgcatataa agatgttaac
     1441 agaaaattac gtatctaata ttcaaccaca gaagtatgct aatacagcat ggtattggtt
     1501 atcttacgga cttgcccctt gcccatatga tacctatgtc ttatgcaaac tcggacccgt
     1561 gcctatgttg tttctattag gaggtattgc tacgttagga atacttgtaa taagggaaat
     1621 tggattcggt agcagcccca gcaggggtag caagtttaat ctattctttt attctgcaat
     1681 taagaaattt ggatcaggaa agtagcaagg ggagtccgac aggcaactat gaggcagcaa
     1741 actttaaaat ttcagatgac aatggtactt tagtgaaaga cgaattaagg tcaagggtct
     1801 ttgaagagac tactcctggt accgggaata taacggtaga aattataatt ccgacgctaa
     1861 acgaggaaga aaccataggg gaattgatcc gcaatattca ctcgtcatgc ttgccctttg
     1921 atctctctat attagtgata gacggcaggt ccactgatgg cacattggat atctgcaaaa
     1981 gagaaaaggt gagatttatc attcaaaaag gaaaaggaaa aggaagtgct atgcgggaag
     2041 cagtagagct ttcagatgca gatattattg tcttcatcga tggtgacggt acatactccc
     2101 tggctgattt agaactgctg ctacagcctc tattgagcgg tgaatcagat attgttatag
     2161 gatctagagt actaggaagg cgagaaaaag gagccattcc aatcttcaat aactcgggaa
     2221 ataagatatt caatagagcg atcagttttg cgatgaaaac taccattact gattctcttt
     2281 cggggtacag agctctattc aggaaaacat tcagggatct tcttctgata agtgacgctt
     2341 ttgaaataga agtagaaatg acagtagaag cgcttgccaa aggatatagg gtgctagaag
     2401 ttcctgttag ttatgggcta agaagaggat cgactacgaa actagaccca ttaagagatg
     2461 gtatgaaaat agccagaacg ttacttttta ttttgatgaa tctgaatccg ctaaagtttt
     2521 ttgcaattat tgctcttggt ttttttgtgg caggcatata tccggccgtt caagttctac
     2581 acgaaaaaat atatcttgga cagatcatat caatgccttc ggtggtccta tcatcccttc
     2641 tctttatgac aggaacaata tccttggttg ttggaatggt gtcggaactc gtagtaagat
     2701 ccagaagaag gttagaatat ctaattaata ggaacttaca aagataaaca attttaggtt
     2761 cattttcatc actttgattg gtaacacctg caggtgatcg aaacttggta tatctacgaa
     2821 cgcaatgatg attcaacaag ggctaggact cgcagacaag aacaagaaaa tcatatagta
     2881 aattgaagag aatttactcc acagatatat atcaggcccc gatctattcg gtgtgcccaa
     2941 ggaaaatata atatgaatct ctatgattga tgtaccaaga acaagaggtt gtgatagtat
     3001 tgcctgacta tgttggtcat gaaaatctag agctcatatc gcaaagtcac aggtttaatg
     3061 actggatgta tggagaaata aaacctgtct tgcgcggaga tgttcttgaa gtaggcagtg
     3121 gaataggaac attttcggaa aaaataattc gcgatctgcc atcttccaat ataaccctga
     3181 cagatgcttc attaacttat gttagggctt tagaagaaag attttctaat gacaatgaca
     3241 acaacatttt tacctataag ctagatctaa attgcaaaac agattatgaa caaataggct
     3301 atgaaaaatt tgattcgata ctagcaatta atgtcttgga acatataaaa aacgatgagt
     3361 ttgctttaga agaattatac aagatgttga aaagcgaagg atcgttggtt gttttggtcc
     3421 cgtgccataa attcttgtac aacgtgattg atacaagtat aggtcatttc agacgataca
     3481 ccgaaaagga tttagagtct aaggctagga aagctcaatt cactatcgat cgaatgttct
     3541 attttaatat gttaggaata gtaggttggt acataaatgg gaatctagga cggaacccga
     3601 aaataaattc tggtgcctat aaaatttttg acaaagttgt tccactcatt aaatattctg
     3661 aaaaaataat tggcaaaaaa atgggcttat ctctcatttg ctatctaaaa aagaacattt
     3721 cgacaagata tactttagaa actatggaat agcggtaatg agtagagaag gggcatacat
     3781 gtctggtaat agtcactatc gcaaatttaa gatgatgtag ttttggtcgt actaagagat
     3841 aattgcgcag gtttaaataa ctggagtcca attgttggga tgtatcagaa ggctgttaag
     3901 aagtagaatt gaatctccta aataataaaa gtcttgtcct gatttg
//
LOCUS       DAOR01000072            2848 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_117413, whole genome shotgun sequence.
ACCESSION   DAOR01000072 DAOR01000000
VERSION     DAOR01000072.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_117413"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    340..572
                     /estimated_length=233
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          818 a          472 c          504 g          821 t
ORIGIN      
        1 accaatgagt cttaggtgaa caaacaaggt tagggggaat ggaaagtaag gacgtggtag
       61 catagtcccc tagccttgtg acacgttagc tgtaaattat atgtggcaaa aagatttatt
      121 tattttttat gtccgactga ctagttatgc aaggagaggt tcttaggttt ggttaaggta
      181 agtccgaata ttgacaaaat gaaaaattag gatggctgac tacggtggtg atgaacaagc
      241 gctgtttact ataatagccg tgaaagtatc cccatccaga gtatgatatt ttctcgtgct
      301 ctggccattt catacctagt caagatgtta ttgctctcan nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctagtcaa gatgttattg ctctcaaagt
      601 cgtttacctc aattaggtaa gatatcgtaa aaagttaact cttccagctg ttgtttggtc
      661 tattccatta gatatttttg ttgcaatata gtgcactgtc acgtagaact aatgcacata
      721 ggcatcgtta cattatgtta aaatgggcta aatcaagcac ttttttttgt ctcaacatga
      781 attcttcatt aactaatata tttccatact ctgaaactcc gaatcagctt tatcgggtca
      841 ataactcatt tgttcctcca taagaaagga gatgttgaga aagtgagaag aaaacaagat
      901 gcttaaagac tcactattgg agagtgtcat tattcagtat tattttagaa tacgccttta
      961 taggtgcatt atatgaacaa cagaatatgc aaaatttggt gctctcaata ggcgttgtaa
     1021 ttgctctcgt tacaggcctc ctgataccag gaatgctgac acattttgca aatgcttact
     1081 ctaccgctac tacaaatttt tcgataaata aggaaatacc agcccaagtc aagagtttca
     1141 ttcttaatca gatagttaac aaatctaaag cagctatcgt tgtgggcttt gtagatccta
     1201 ctggcactcg catattcagc tttggaaata tgtcaaaagc acataacgta cctgtaaacg
     1261 aaaacacatt gtttgatatc ggttccatca ctaagacgtt cactacccta cttttagctg
     1321 atatggtaga gcgagggata gttaatttga ccgatccaat tgaaaaatat ctgccagcta
     1381 gcgtaaaagt gcctgagttt aacggaacca agataacact tgaagaccta gcaacacata
     1441 cttcaggact tcctgattgg ccttctaata tttggttaaa caataatgtt ggagatatta
     1501 acccaaatta taatgcaaac caattgtatc aagcattatc aaatactaaa ctaacaaggg
     1561 aaccaggttc acaagttcaa tattcaagtt ttggcattgg attacttgga cacatattat
     1621 cgaagtcagg tggtattcca tatgagcagt tagttaaaga cagaattcta gacgtgctag
     1681 gaatgaatga cactaaaatt actctttcac agaatgaaat caacaataga tttcccgttg
     1741 gccatatggg ccgaaaagaa attaccactc caacaatgcc tactataatg gcagattctg
     1801 gagcatttcg ttcaactgct cctgatatga taaaatatgt atctgctaac ttgggtttga
     1861 ttcatactaa actagatgct gcgatccagt tacaacattt aatacgacat tcttccataa
     1921 gtgtaaatcc aatgaattat agtgaattca gaggattagg atggcgtgta cttactaatt
     1981 ttggagcgga gaccttagct catactggat ctatcaatgg ttggaatgct tttgtaggct
     2041 ttacccctac aaaacaagat ggtgttgtat tgttgtgtag ttgtgattct ggagacgccg
     2101 atatgggtag cgttggtttt gtattattgc atctaacagg aactgaaaat ctgactgcaa
     2161 agacagagcc gagccttcat acaaccgcag gtcttactta aaatcaagcc cagatactgt
     2221 gtgaagctca gtggtattcc agtcatagaa ttttattctt gaagtaatcg ctgcatttgc
     2281 cgtaagtcat cgttgtatct tctattgaaa ctggaatgct gttagatcac agtcagtctg
     2341 gcgagtccag tgctcttgct cattgaatag ataacgctga tcttatcgta atgattatga
     2401 atggattgga cacctcttca tgtttaactc tccgagactt gcgaatattt tgctcgcagt
     2461 aaaataataa cctacagttt agtaagacaa gcttactaaa taaagggtaa agaaagttac
     2521 gactgcatag tgtgtttatg tctggttctt actcgtctag acatatgtag gagtaagtta
     2581 ctcccagaaa aataagaaga tatctttatt ttagctgacc cttatttttt catagtcctt
     2641 cacttttcaa atagcaaaaa ttttttcgac ttctgaaatc gttaagacgc cattcgttat
     2701 cctgggtgcc tctggatttt ctttaaaatc atcttttact agaagcactt gaaggagaag
     2761 tcgtacttgt attcaatgca cttgtatttg atcctatagt agcaatagta gcaaaggaat
     2821 gtgaaagacc agaatgagcc tcaagcca
//
LOCUS       DAOR01000073            9364 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_49707, whole genome shotgun sequence.
ACCESSION   DAOR01000073 DAOR01000000
VERSION     DAOR01000073.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_49707"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3063 a         1652 c         1844 g         2805 t
ORIGIN      
        1 gtattttttt cgtaatctct tgccatcctt ccagtatgcg taaagatatg ggccatgcaa
       61 ttgttggcaa tcaggattac cacatctaac ctgttcctgt ctgattgttg cattctctgg
      121 aatttcgtat tcattatctt tgttcatttt tattcgatcg ctaccacttc tgttttacac
      181 ttattgttat cgtacttgat agccatgttc tgtcaagtag ggcttacgga ttacatttga
      241 aatgagaccg tagagtccta tgttttccac cttcaaaaca gtaaaactaa ttggtaggta
      301 gccaattctt attgcagatc tcaagtcata cttgattcgc tgatctggat taaaatccaa
      361 tttccttata ccattacatt cagcaagcca ccctttcact attacaaatg cctgatcgta
      421 ggacaatttt ttgacattca aagagtaagg tgccaaaata cgctaaagta caaattttct
      481 ataatcccct aatggagttc gaagaagtat ttctatccag tcagtgttgc gattggtatt
      541 attctgatta ttgtcgtagt gagcgtaaat tcgcctccta ttaaattgga cttgaaaaga
      601 aaaaaaggcc atagctacaa gtaatagatc agatagaaaa tattacgaat gatttccgag
      661 agtctcattc tgatcttaac ataggtacta ttacaacacc tgaagaatta ataaataagc
      721 aatttggtaa tagaaaatta gaagaaatgt tagtttagag actggtttct gagttagcag
      781 tgagtaaata tcggcaggat agattaaaaa ttctatctaa caatgacaga tttatgagat
      841 tggtatttga tatgcggcgt gtatttaata atggttatga ggaattaatg aaatttaggc
      901 atttatgata atattaatca tttatccttg aggatcgatc tcctataatg ttactattta
      961 agtgtccgag gcgatcgact atcttgattc caaacgtcag attgtgttaa tccctaattt
     1021 tcacttcgga acggtttgta tccatggcgt tcaatgcgct taggatgcgt tgattccttg
     1081 atttcgtgtt ttagtgtaga agatctatag ctttatgttc agattgtaga aaatttttta
     1141 gatatcgttt gctagtcatc ctgtagtatt gtgcattccg ttagaaggac cgagaaagag
     1201 ctttcctgtg gatgtctagt gccattaaag tcaaacatgg ccgaaattga attagccacc
     1261 aaaggctttg catcaaagga ttggtttcca attcgtccca ggtgtcagtt atcttcgata
     1321 aaacgcagga tagatgattg gtcaggtagt gtgtgactag ctaacaggtc atatatcttg
     1381 aaaatgaacg aattacggtt aacttatttg agaagccgcg agcacctggg agaaaaaaga
     1441 tagaagaatg ggaaggtaga ctactgtaac gagataaata atgcaaatct gacggtcatc
     1501 atgatccctt ctctaataat tattggactt tttagaatta cgaccttatt aattggtttt
     1561 tcataaataa tgagggtaca agcgaatatg agaatcatta tataacacgt cacacatcaa
     1621 acaaaggtct ctatataaga acagggcaga agatgatatt attaacacgg caaggatgga
     1681 ccattatgag actttattga ccatgtgatt tatcgttagg tgtttggaga gtgtggctag
     1741 aaaatatcca aagacgatca tgaagttcta tagcatcatt gtataatatg cctgcttatt
     1801 ctcaaaatta tggtcaaact tcgtatacat cttaaattga aatttattaa ggagatatgt
     1861 atgtatataa aatggaggat gttaaagtct atcaggattc aagaattttc ttctagatct
     1921 acaaggtgac aactatgtct gaagaaatta aagaaatcaa gagagtatat gcagggttag
     1981 agacactata ccacacttat tttccaaaat cagatccatc tttaattgat gatttgctga
     2041 ggcgtctgca aaaaaatccc aacattactc cgatatatat ggtggaagtg ttcacaaaac
     2101 caggcgtaaa tcctgaagca gcaaaaaaat atatctttga taaaaccagc atggttcctg
     2161 caatctacga gaatggaaca ctttttgtag ctaatcacaa actaaccttg gagattctca
     2221 gagaaatatc taattctgat gatgtcttag aagtagctgg cgaatatact ggaaacataa
     2281 catctacagg atcatcccac gtgcattcag atcattgtcc tctacgcaac cttgactacg
     2341 cctactagga aaatcgttgt gaaatcaaat ttatgtagct tttaatactt ttaatacgca
     2401 ggattttatt taacatgcaa ggaagataat gtcattaaat attacagaaa atgatctcgt
     2461 gcggtaggca tggacggaag ttattgccag tgtatgatcg gacctgtggc tggtgcattt
     2521 cttagataca agatagtttc ctctccaatg atattaggag ccattccagt taatgtatta
     2581 tttgtagcga taatgctaaa gaatttcagc cttaaaggca acgtatgctt taaaatgctt
     2641 aagtgaatgt caaagcatac atcagatagc aagggctttt aacgattttc ttatagtaac
     2701 agaattggtt atcacaggag cagcattctc tccatctcac gattcgcctt gtaacgcttt
     2761 aacattctat tccttcaaag ttgtttagat aggaagattt ggcaagataa gagatagact
     2821 tgccaaaggg ttgttgttcg aagcaactac tatatatttc gtaatactaa gatgagaaca
     2881 tagagatgtc aactactact aaaacaacaa tcacatttca agaattagga ctgagtcctt
     2941 taatactcaa agcactcaaa gaaaatggat ttgaggcccc ttttcctatc caagaggctg
     3001 ctataccgtt acttttaaaa ggtgtagatg taatcggtca ggctcacaca ggaacgggca
     3061 agacagcagc ctttgcacta ccgatattga ccaagattaa gattaggcgt aatggtccaa
     3121 tacaagcact gatacttgtc ccgacacgag aactagcttt gcaagttaca aatgaaatta
     3181 ataaattcgc aaagtacact ggaattagaa cggttgccat atacggggga caaagtatcg
     3241 tccttcaata taatcagtta agaagaggca tacaaatagt agtcgcaaca cccggtagat
     3301 tgattgatca tgtaaagcag aattctatcc agttggatga ggtaaagttt gtcgttcttg
     3361 acgaggcaga tagaatgctc gatatgggct ttattgatga tataaagtcc atattgtttt
     3421 atgtaagtga agacagacaa acatgcctgt tctctgcaac tatgccacca gaaattatga
     3481 gagtagctca ggaatatatg catgatgcta aagaggtaat gttgaatcaa gaagaattaa
     3541 gtcttgacac tatagagcaa tcttatcttg ttgttcctga aagagaaaaa ttcaaacacc
     3601 tttgtaattt cattaagaaa agagataaga aacaaacaat tgtttttgct gctaccaaac
     3661 aaagaacaca aagacttgca aacgaactaa aacaagaggg ttttaaagca atcacaatcc
     3721 atggcgatct ttcacaaagg gagagagaca atgcaatgca tagattcaag aaaggtgctg
     3781 atgacatact ggtagcaacc gatattgcag caagaggtat tgatgttcca gcagtaggtc
     3841 atgttatcaa ttacgatgtt ccaattgaac cgttgatgta ctttcataga attggaagaa
     3901 ctgctcgagc tggtgccaca ggtaaagcca tatctttagt atcacatgat agggttgacg
     3961 attttggtag aattttgagg aagacagaac aaccaatacg gaggcttaat gaagagatgg
     4021 gaattgcagt tcctactact caaaagcgtc caaataatag atatcaacca agaccaagtt
     4081 attcgcctaa cagcggccac agaagtcgtg gtgattatgg cggtaataga aatatgcgca
     4141 gtaatagaga acagcgagat gataatcgca gtagtagata tggtagagac aatcgcagta
     4201 gtagatatgg tagagaacgg ggtagaagtt attcctagcc gaagaaataa aatcagttat
     4261 ttatctcttg gtgaaaatct tgtgatttga taatcctgga gtactactag ctgtgctgtt
     4321 aatctggtta gctaaagaag ataatgttgt agggggatga agaatagatc ctgacatggt
     4381 tgtgtttgta tttttagtag aggatgtatt attaggagaa gatgatgatg caaacactgt
     4441 tgccgtaaat agtgatgatg aagctactgc catggctatg cctaaaatca atattgctgt
     4501 tgttgctaat aagatatttc tcataaaaga cgaatataac attcctaatg tgtggtgggt
     4561 gatgttcgga gggttaggag ttacttcgta tatgtaatgg ggttgaagta aatgacgcta
     4621 atagatttta gacgattatt tggaatttta tttactatgt ttatcctgat cttggtattt
     4681 atttatctaa tagttgttat agctgagaag ttaagtataa tcacagcctc aattctgatc
     4741 ataataccat tagttattag gtatgtattt tacactataa ctaaaattta gaagtaagca
     4801 agagccctat tctacctcat ttaaccctca aagtgacgaa ctaaccacct ccgtagcctc
     4861 agtgcctccg gaaaatatac catgatagaa tcatacatac atacattatg gcaaacctcc
     4921 gcaggtttgg atattacgga ggtaatttct tgtctgttag agtctgagac tttagaggaa
     4981 agttccgtaa tagcccgcta attatattag ttatagtaga gtagaataaa taaagcagat
     5041 ttgagtttaa ccaggattgc tcactcacct tatacgcttg tagtttgtga aaagcctaat
     5101 gctgcaagaa gaatagcgca agctctagga acctcgagtt tcaaggaaat atctgcttta
     5161 gagatcgtag gaagaagagg actgccacct gtattttctg ttgtagaccg taataatcaa
     5221 cactttgtgg tatgctcagc actgggtcac ttgtacggtt tggtggatgt taacaggaac
     5281 cgaagtacgt atcctgtgtt tgacgttaaa tggatgccag ttttaaataa gagaaatcga
     5341 aaaggcccaa agacagccac aagatctgta cagatcataa agtcgatatt cttgctttcc
     5401 caaaaagcta caagctttat tcatgcttgt gattatgatc aagaaggtga agtaattggt
     5461 tataatgttc tcgaatatgc ctgtaagaac aaatatgaaa gttcactaag agctaaattt
     5521 tcaactctca cagatgaaga aataagaaat tcatttgata atctgttaaa accaagcaaa
     5581 ggattagctg aagctgggag atctcgtcat atgatagatt ttatttatgg ggttaacctt
     5641 tccagagccc ttacacaatc ttttatggtc agtaacgatc gcaaaaggta ttgtaacttg
     5701 tcaataggta gggttcaggg cccgactctt gctttcgtgg tggaccggga gatggagatc
     5761 acacgacatg tcccagaacc gtattggaat atatctggag aatttgaaaa aaatggacat
     5821 ataattaagg tgcattatta ccagcaaaaa ataagcacac tatctgaagc cacttcaata
     5881 gtcaatgcct gcacaaacga agacggtaag attaccggaa tagaaaatca aaaggtcacg
     5941 ttcaaagctc caactccttt caacctcggt gatctgcaga aggaagcata tagggtattc
     6001 aaattttcac ccaactatac actaacaatt gcagagaaac tatacattgg tgcactgatt
     6061 tcttatccca ggacatctag ccaaaagctc cctacgtcaa taaactacaa gaaaattatc
     6121 tcaggtcttt ccaaaattct ttttcttgtt ggcagcagtg gtacctatac tagcctcgct
     6181 aagagcctgc ttgcaagaga caatctttct ccaaatgaag gaagcaaaac ggatcccgcc
     6241 catccagcaa tttacccaac aggggagaaa ccaaaaggta aacttaaggc tgttgaagtc
     6301 aaattattcg atctcataat cagaaggttt ttgacaactt ttggcgatcc agcgataagt
     6361 gggcacacaa cagtcacgat tcttgttaag gataactacg ttttcagagc tgacggaaag
     6421 aaaatgctct acgagggatg gatgtatttt tacaaaccat ataataatcg atctgacatt
     6481 gaaactgatt tatcagagct acatgaaggt gacatactga gaaatgtggt tgtaagaatg
     6541 gatgagaggt ttacccaacc tcctcccaga ttcaatcaag ccagcttact ggaagaaatg
     6601 gaaaaagaac agattggcac taaagcaaca agatcggagg ttatcaacac tctctttaag
     6661 agaaattata ttagtagtac tagtactcat caaagaaaaa ataatggaat aggaggaatt
     6721 gaggctacgg gggttggatt tgcaataata caatcaatgc gcaaatacat accaagtata
     6781 gtatctacga agcttactag atctatggaa gaacaactag aacaaattga gtcaggcaaa
     6841 gccaagagtt cgcttgtaat aggttacgcg ataaataaaa taaaagaagc agtagtacct
     6901 ttcaaggaaa aggagactga gataggccgt caaataactg atgcagtaat aagtactcag
     6961 aataagcatc agcagagggt actagggaat tgtcctacgt gtagtaaggg agtcctaaaa
     7021 atcataagat cgagtgtaac aaaaaaaaga ttcgcaggat gttctaatta ttcttctggt
     7081 ggatgtaagg caatcgctcc actaccacag aaaggatcaa tagaaactat ggacaagacc
     7141 tgtgatctat gccgatggcc agtagtcaag accgtctttg caggtcaggc aaaacatcac
     7201 tgggaattct gtattaatat gcaatgtccg tcaaaaaagc gacagcaata acccccccta
     7261 aatagaaatt catttttaga gaccagatcg acaacgatca tcaggcaagg tgactctgta
     7321 acaccagagt tattcgaact tgagttagat gcatggtgga acaaacatac atgcaaagcc
     7381 aattaccacc aaccaaacca cctgaacacc aaaccaatcg gtgttcttgc gtaacggata
     7441 gaaaaacaag gttcgacttg tatcagaatc gttctagaat aatgaagtag gtattaagac
     7501 gtaataaagg ggctttggca ctcgatcaag aagactaaaa caaatacaac tagcccagat
     7561 gagaatcata ctgtagccga aaaacagatc ggtttcttat gtggctgtat aagttctcac
     7621 gacgggataa caagtttgaa tgtaggtgat taaaaggtgt atctatctag gatatttgtt
     7681 gagaagaata ataggtcatg gaaagtatta ctactgcaag agttgaccca cgccttatgc
     7741 atgtttgcat tagttggtat tctaatccgt ctgcaatcat ttggttgact ccatattatt
     7801 attgatatat ttcaaagttc aggtggcgat gcacccgttt gaaaaataca taagaattaa
     7861 atcttaatcg tgacatccac ctagcggtaa aatgcatatc cgctgcatct tctagatgag
     7921 ccaaaagtgt gttccataat cagctgtcca ccacggccaa tcacataaag cttggaacta
     7981 ccatgtttgt cttcatgcac attgccagta atattgattc gttgaccata gacatctatg
     8041 gatagtatag attcttctgg atagcctttc atgtaatctg aagtcatatc gatttgttcg
     8101 ccattgcatc ttattatcaa attagacatc cacgatattc taagaccgaa tgaatgtttt
     8161 atacgctcaa tcacattttc aggagtaaaa tctttgttat ctctgtatgt gttgaagaaa
     8221 gttactttag tgccttgtcc atcaatgcca aataaatttt gtatttctgg agttattccc
     8281 ttagtctctg ggtcactaaa gcaagcctcc tctttgccct tttcattttt ctcactatat
     8341 atttggaacc tatgaataac accgtcatgc agagagtcct gagataccac ctccattcta
     8401 tttgataaca agaaatagag tgacatacca agccctttat gaccacaact atagggattt
     8461 tcttctttta gtgattctgc tttctttgca tccagaaatc tagctatctt gtaatcatat
     8521 tccttggcta tacttcgtcc tgagtcctga attgaccaaa gaataccccc acctggaaaa
     8581 ttaatatcaa tcatattagg tattgcatat gcatcagact ggctgatatt atcgctaatt
     8641 atttcctgag tagtcggcag tgttgaatcc atagcatttt gaaacgcttc tgttacttcc
     8701 tctaatatgt cagtcgcagt tttctttatt gcatctaatg cacccgcatc atcaaatctc
     8761 cactttttac attgtgataa tggcatagtc attttattat taattaactc ctatgccagc
     8821 taatgcttta tggaataccg cattagccat agcctcagtc ttaccattct ttttcttatt
     8881 attaccctct aattgtggaa gtctttcctt aagtccatgt aaataactta ctatcttaat
     8941 taagtacggc atgtcatatt tctcgacatc ctctatcatg taaccctctg gtttcatttg
     9001 atagattacc tcatcgtcgt tattaacttg cgtgaaatga cacacgtctt ttgggaaatg
     9061 acgctcagtt tgtgtgagct gatgcctctg tgcggcactt tttgcctctt tagtttcctc
     9121 caaatctttt tggggacgta gtggtattgt caaagtctgg tcaatggatt gtattctctc
     9181 agataatgct ccatgtgaaa tagtggctat gataaatgaa aatacattga ccaatagtgg
     9241 gagcttttaa aagatggttg ttgaatatcg acaaagatgt agattaaaag acgttcatgg
     9301 tggtgaacca aaagaccgaa tacaggttca taactggtta gttaattgtt gtacttacac
     9361 acat
//
LOCUS       DAOR01000074            5366 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_67375, whole genome shotgun sequence.
ACCESSION   DAOR01000074 DAOR01000000
VERSION     DAOR01000074.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_67375"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1560 a         1086 c          978 g         1742 t
ORIGIN      
        1 accactattc catccacaaa ctcacctgta accatcccca acgataacaa caacctcatt
       61 cctcctctct caaccactat tccatccaca aactcacctg taaccatgcc caacaataac
      121 aaccacgttc ttcctcctcc tactcctccc tcaaccacag ttatgtccaa atcagctgat
      181 aaggttccaa tcctcctccc atttcattaa tcatgaaaat ggcggttgat acgtaccaat
      241 ctttgtacga gtctgtggaa tcgtccatag aaacatatct gagtaatctt acttctgata
      301 tcgaaagtcg ataagatacg tacttggtag tttaagcacc tcgtccagct cgagtggatg
      361 agatcaactc catgctagag tcgtaatgac cgattgattt aggacatttt acgaacctgt
      421 caaaattcga ttgacgaata gtaattaaga gccgcgctga ataatcaatc gtaggcaaat
      481 cattgcgcaa tattcggaaa tatattagat tgttagatgt tctagcttcg catcaaacta
      541 tcagtacttg gattgaaaaa gtatgttcat ttgatgcaat tacacgtgtg caatagttgg
      601 agagcaatcc tactcaacta agaatctatc aacataatca gcgttgtgtt attagcattt
      661 gcttggtatt gtccctaaca cttttatgat ttgcttacag ccgacgatac actctttcag
      721 atttatccaa taatggcgtg tagttcatag ggttagtaag agagtgtcat tttctagtgg
      781 ttaaagttac actcctcgca acctgtcacg gaatgtgcga acatctatag tccgacaaca
      841 cgtctccaaa tgtacatttt atttcgacgt tctttccagc aggatttgat gtggatccag
      901 ccataataat attgtgagtt tcattcgtaa ttttttgatt tatcgtaccc gtgcttacaa
      961 atggatttgg aagtgttact tgagaaccgt tattcggaga ggttgcggct atgttgttgc
     1021 tttttacata tccaatattt gggctgctgt caaagtcatt tattatcgtt gatgttatca
     1081 gatctctgga cgcatttata gatgagatct cgccgcttat cgtataattt gtcaggaatt
     1141 tttcgacttg gacagaagtt gatggaattg aatatacatc gattgaatta ctggttaatg
     1201 ttatgccctt atttggatta ggggtgccat gatcttgatt ggtaatagta cattgcatgc
     1261 ggccgccagt agcagtacca gaacagcctg aagaatagct aggactatat cccgaagtgc
     1321 cggattcagt aaccttatag ctcccaccaa acaatgtaac agaagtacca cttgatgacc
     1381 cggagaatga ttttggtgat ggattattgc cagatacagt tacggtaaag tctgaaggtt
     1441 tctttgtgcc tccattaacc ttggttgtaa ctgtcaagaa tgtctgtgag cctggaagag
     1501 gtttaggggg ttgagtagaa cttgagctta aagatgggat aatattacta ctggcagcgt
     1561 ctatactacc aacgctagtc agagatattg ccaaagtgat gagcaagata acgtttccta
     1621 taacgaccgc agttgacctc gaattcaaga agagtccact tttcatgttt ggtagcatat
     1681 acgaaatcca cgctactatt tatgctaatc atataccaaa accagaatag tcttaggaat
     1741 taagttttta ggctgatcgt gtaaaaatta aaataaaatg ataatttcaa ttgattccac
     1801 cttttcttgt ctacttaatc ggacagctat ttatggaggc cgtttttggt aatctgctgc
     1861 aactggtgac tgccatatac atctaagcga gtagaactgt aggttctctc ctatgcgatt
     1921 tctacatccc ccagcaacag gtttttgata tatttttatc tgtctcggct agtgcatatt
     1981 ccaggcttca tacattacat tctggacact gtcgtaaaat gtgagttgtc caactatcaa
     2041 tttacatcca gagtaaacgt ttcataatac atagttacaa atcaaataga aaaaatttgc
     2101 ctcagtgcta ttataatggc tcggcattaa cagtatcatt aaatgcagaa gcagtcgaga
     2161 ccacttgggg tcagtatcat tgcaaatttc tatatagtag ccggcatcat agggattgtt
     2221 ggcatttctc tctcaaccac tggtgctgtc aaagcatcag gtgttggcgc tgtatcgtta
     2281 gcggaatctc tttacattct cgttacaggc tacggattgc ggaagggtaa aggatgggca
     2341 tggactatat ccattatcgg catttttatt ggatttgcta cgataacata cgcaataatt
     2401 ttcaacctag tacctatcac aggctcaata ggcaatcaag ttggaagtgc aacagcaggt
     2461 ttggtgataa acgtaatcat aatcagttag tcataattta tggcatcgaa ataataatat
     2521 tgtattattt atacaggcca cgtgttaaag catatttcgg taagagaacg ccaaggtcag
     2581 catcgcctag cacccagtaa tagagtaatt caataccttc taattagtcc tgcggcggcc
     2641 aggacatact ctgttttcct actcattttg atctatctat ttctgaaagt tctgatcgcg
     2701 gcattgggag tttaaattaa aattcaaaga attcatcgga aagaatcttt tcgatagtgt
     2761 gtatgcttta gatcgttggc tctccttctt gcgaagtaag aataaaacgt ggcattagct
     2821 ctggcacagc ttctggaacg aaggtttgaa tctgatatca ttggaaacga taaaccggta
     2881 gtaccatttt tcataatttg aaagtaatca tttgttatgt cggcatcttt ctgtgttgat
     2941 attattattg ttctttagaa gatgcggacc tttcttatga tcttagcact caaatctact
     3001 agcttagaat ctatctgagc aacgaaggtt gttccgtcat taaactcaac tctaattcct
     3061 tctgtagaat atctatcatt tccattcttg taaaaaagaa gccctttgct cttattctga
     3121 tcttgtgtgt tgtttttgtt tctattatcc ataatggtta tcttcctaat tatcgaagtc
     3181 agatattata tattggtaat catacctcaa aaagcagtta ttaccgtctg cgtatccaga
     3241 accactagtt caatcttatc cgatcgatat tgttatcacg caacactatt atcatgaagg
     3301 atcagaatta gatataatct tgggcgtagt aaggactgcc ggtagtattt tcattggaac
     3361 agcggtgtta ttaatgataa tcgtccttat cttctttttc ttaaagttga tcacacccgt
     3421 actattgacc cttactggct taatcctcct aatcattata atttgcatcg gattatggtt
     3481 gttgggaaag tttcgatgat tcagctaatt ctctcacacc ctagaatcta cagttacacc
     3541 aaacggccgg ttgcaatttc acgtaacctc tcgttttatc attttcctaa atcctttgcc
     3601 gaatttcagc aaaaacctag gtctttgcgt tacggtggta tttctgccca aaattatcca
     3661 gatatgaatt aatgacatta tcgactaaca ggaagcttga atgcaatctc tgacactact
     3721 gtgacggcca caaatcgtga tgtcgatgca ggaagcaagc agaagtcaga atgcggagca
     3781 gatattataa aattaatgtc tgatgacaat tcactgttta tctttaacac catctttctt
     3841 gcgagtggag ataacagcga gactctcagg actcaattga aacttactaa aaagcagtac
     3901 tattcgagaa tatctcgtct cacaaaagct ggtttagtga aaaggcaaaa aggcagatat
     3961 tttgttaccg catttggtaa ggtggtctat aatgctcaaa aattacttgg aactgctatc
     4021 aagaactatt ggaaacttcg agcaatcgat accctaggag tagcaaataa taatattatg
     4081 cctacggaag aacgtagcaa gataatagat ttgatgatag gcaatctaca aatcagagaa
     4141 attttgcttt caacaaattt ctaaccatgc tgcagagaag atcaggtaac tggtgtatga
     4201 tttaccggcc ctagccaaaa gttctgcctt cgaaattccc ttaaccctgc aagtcactca
     4261 atggattgtt cgtgcattgg attaatgtct tttattggtt ttataattgc atttagtttc
     4321 tccaagcctt ttacaactct ttgtcgtcgt gtctaccaag gcagtatgtt caaatggctt
     4381 ctgcattaat tcgacaactt gtttcaactg ttttaccgta tctacaagaa attccatgat
     4441 ataggctgaa gcaagtatta ttctctgata aggatttact gcaagaattt cttttgaaac
     4501 atccatgcga tccttctttg gcgtcttata atctaaatca acggcatcaa aataactatc
     4561 aaatttcaat attttcagaa ctgtcgtctt tgctcttcct gttgttgttc ttcaagtaca
     4621 aatttgtcaa atgcctcacg ataactcttc agacatgcct caccgttcta aataaacaaa
     4681 acttcatgat cactgttttt gcaaggaaac cctatatatc atagcaatac tgtattcgtc
     4741 ttctgcgatc ggaactttca tcccttgatg atagatatga tagccttgat gatagattca
     4801 atagcattat tttaattttc acgatttcaa tcttcgtcag cacgtcggat catttagatc
     4861 tctatgatcc ttttcaaatt gtttggcatg taaaaccaat cgtgttttgg tttcgatcgt
     4921 caacctaatt tcttgaaatt gagccagtta attaactagc gaagaggtaa aagaagtgat
     4981 actagtactt gctccaaatg ttcattcggt acaggcctcg gccttgatag tggaccttca
     5041 gctatctgct tctcatatag cccacatgct gcataattac ttacgtccga caacgattta
     5101 ttttcgcttg ctcctccatc tctctccgag cgcatccact gtcgaaggct cgcgtgtgcc
     5161 aaccaaatgt tgtgcgctgt ggctttctat aatttctcga gcatcctttt ctttttgcta
     5221 tcagcataaa attattctga tcgcaacgcc cattatcgtt ttacacgttc ttattatcaa
     5281 gactaaaaga gaccggctta tggaaagaga gattctgaat acgagaatta tgaaccacac
     5341 aatagcaaac tattctctca cttctc
//
LOCUS       DAOR01000075            4335 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_10479, whole genome shotgun sequence.
ACCESSION   DAOR01000075 DAOR01000000
VERSION     DAOR01000075.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_10479"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3713..3901
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1142 a          845 c          752 g         1407 t
ORIGIN      
        1 atcttgtcgt ctttccttca ttttctaagc gcgcaaactc ttttgaagag tttagtgcta
       61 acagaacgct acgcctgtct ttaataacaa attcactctt ggaaaatact gaaatatctc
      121 cttcaggagt catcaccgtg ggactcgatt cttttaatga atccaaataa tgcataattg
      181 acagactttc atttcttaaa tactggtcaa aagctatatt tcgcgtatta tttagttggc
      241 gaaattctct tgagctctca aagttatgca aaacatactt tttaatatga ggcaaaaagc
      301 gacgagacag gacaattcca gcccctccaa gtctagttat atttcctata tgatatggtc
      361 ttgaatatag aatgtcatat ttgttcaaag attgggaaga tgatctatca tatacaaaaa
      421 actcatttgt ttcattgaaa atatctttta ccttataacc tactcctctt ctaatatcta
      481 aaatttctaa aaacttcata acagagtcag accatgcttt gagagtctct ttgtccagct
      541 cagcaagatt gcttccatat tctttaccaa ataacttgat tatcgtatca tttttgttag
      601 gtacaatgta atcgtgtatg taccaatcat aaaacaatct tctatcacca aggtcgcttt
      661 ggctattcac accttttcca taagatatca gggcgccttc tttgtcaaag aattcgttac
      721 agtattcgtt aattcgtccc ctaagtttgt cctctaggac atcccatgtc gctcgttctt
      781 gatcgaggct cagacaacaa tgtttgtatt tcttcttact accacatgga catggggaat
      841 ttctgtcata tttcaattta caaagaatat aaacacacaa ggtgaaaagt atttctgaag
      901 ggtcaaagac ggaacaatcc attttgccaa cagcatattt ttggaaaagg atatgcgatt
      961 aatacttact tagctgtggc acctatgtgg gtatttgaaa gtgtcactta tctatgcaag
     1021 tactagactt agcctccaga ttcgaatcgt agaggtagat gtgcaacact gtacagcggg
     1081 gtattcttct ctctaaattt gagccaaatt cgacctacat atcttaaacc catttccaag
     1141 ctaaatcata gtagtggctg tctcctagtt ttattactca caataacggc ttgaactgca
     1201 gaagtaaact cttattcact cgcttggtac aaaatgtgat tttattgttg attcgcaatc
     1261 tttccttttt tgtcgattat gttattcaac cagtgaatcc caacagtagt tattctgtat
     1321 accgtgcaag gttcttcttg tccttctccc gcactttccg gactaaactt caaatctttt
     1381 gttacatatc ttgatttcat catttccgat aacctcgagg ttaatgattt ggaattggct
     1441 ttagttgcag actcaatgtc agatattttg ataccttcat cttggattat accgcggcct
     1501 ttagcaatcc ttgatgccac gaggtgtaga gcaataaact ccttgataga ccattttacc
     1561 tcggctttgg ttttagattc atctcccatt accttatcaa ctttgaggtc tgattcgttc
     1621 gctggtggca tttcttccat tttgtctgtt gatgatgtct cgggttgaac taacacttgc
     1681 accccttcag agttgatttt aattagtctc gaatacttct cgataagata atctttatcg
     1741 taatttagat atattttcct cgcgagatct acttctggaa attgtttagt aaagaaatct
     1801 agtgtactct taataacgga ttgcagagga cctttgaatt gtacgttcat accctacaaa
     1861 gttattgata cacttacatc tctctagagt gacatcatta ttactgtgta cttttaacat
     1921 gttgagataa tcgataagaa tgactacgat attatattag cttgctatgg gtaagtaagc
     1981 tattgtatgt tttgcggatc gtatcttaat acttaactag attcacttta aactgtcttg
     2041 agtataatcc atatagtcgc ctttcatatt tttgtagttc ctctggcgac ataagcagat
     2101 ggtattcaac tccatcaata agagaatgca taagcctacc tactctttga agttgctgtc
     2161 ttcttgaccc tccaagaaaa tcaatctcga ttatattctt tacatctggt atatccattc
     2221 cttcatcaaa tattctgcta caaatgaacc tcttatgctt gtttagtgct tctattcttt
     2281 ccttcaaagt atgttctccg tatatgaatg gaagtccagt aagcctggat gattcctcgc
     2341 ctgtggtcag actatcggag aatacgatag tattaccttt caaagtttga agtaatgatt
     2401 gaagaaatgt tatctttgca gatgtgacat gcaggtttat ctcaggcttt tttacccatc
     2461 cttcttcaaa gagcttgtcc caatcgcttc cataagggta gcctgaaagt acgtaaatta
     2521 attctgagcg gccatcttct ctataagggc tgccagatag tcctaatctg taatctgcat
     2581 ccagaaaagc gagtctggaa taggtgctag ctggaaagtg atggcattcg tcaaagataa
     2641 gaagtttaaa tcgttcgttg gtaagatatg ttaatgcgct ttcatatgtg gagattatta
     2701 tatcggcgta tttgatcgca tcctttctag ctcctgcgta caaaaatgct atccgaggct
     2761 ttccgttagt gttgttattg ttctttttga gatgctcttc aatatatttt tgccaaactg
     2821 cgatatgaga aacagatggt accactacta gagtcctgtc cgatatgcaa gagatagcct
     2881 gcactccgaa tagtgtttta ccagcgccaa aagggtagac aagagtcatt gcaccatgtg
     2941 ttatgaagaa atccattgct tcttgttgat atggccttag tttgatatca gtaagccaca
     3001 tccacgattg agacactgtt cttgatgtcc acggaataac cccatcagac actagtttgg
     3061 aagcgagatt aaatcttgca gaagctgttg atttgactcg tattccgatg tctttttcct
     3121 ttctcagcag atactctctg tatcttatcc aggcgtcttc aagttcaaat tcattgccta
     3181 caaggtaatc accattctgc aatattttga tcgaagaata cctaggagct aagtccaact
     3241 cttctttaag atcgtccggt actaatccag accacgctga atatctgtct accgtgaaaa
     3301 tgttgtaggt ttcagtctgt gattccaacc accctgctct aaaaccagtc catttgggag
     3361 atatcaatag gtaagctttt tcctctttct tatgatttcc ttgaggaata actgcgtaag
     3421 gtctatccat aaactcctga aaacgctcat ctcttgtgag cagcgaagta tcatcactca
     3481 gattatattc aaggcacttt ccaggctgca tacctagata tgctcgatta taaataaact
     3541 gagggtgagt cttctttgcc tgcaccgaca ctatgtttcg ctcttttatc ctagcttgtc
     3601 caagcgatgg cgatggtatc ttcgtcttgt cgactggcgg tgatagcagc tgctgctttt
     3661 gttcttcgct acctagcgta ttgatggcca taatctaaac agttgaccaa ctnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 naacagttga ccaactgtct ggagggctca aaaactggtg cagaatgtgc ataaagggca
     3961 atcagccatt tgataaacgg ttagggattg cgataattaa agaatatgtc tgcacaacgt
     4021 tctaggcgta atgaaaacag tggtacagtg tactagagct tgttgggaaa tatttcatat
     4081 accattggct atacgtttgc gcatgttcca gtgttgttct agcgcctgca gggctactgt
     4141 gtgcttagca aggaatgtat catttaagag ttatacttat gttgaatccg cataaaatca
     4201 ctatcgtgaa ctattgatat gtattcgcat ggtatcttct tatgaaaacc cttttttctc
     4261 taagacaaga aatcagtcag tcatggttat gcgggttcat tgcgccctgc attccattgt
     4321 ggccgtagtc ttgcc
//
LOCUS       DAOR01000076            4134 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_52449, whole genome shotgun sequence.
ACCESSION   DAOR01000076 DAOR01000000
VERSION     DAOR01000076.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_52449"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1362 a          759 c          896 g         1117 t
ORIGIN      
        1 ctcaataaga gataacttgc caacgcagta gttcgtccga cgaattttta cagccctcgt
       61 gaaccgacgt agactttaca gaaccagacc ccaaaatata tataaaacat aacggtgtta
      121 taatacacaa tggctactaa agaactcaat atggattata gtaagtacga ctttaaggat
      181 tctactgaac tctatgtcta cctgagcaag aaaggattgt ctcggggtac tgttgaagag
      241 atcagcaggc taaagggtga accagattgg atgagagact ttaggctgag atcctatgat
      301 gtattcatga gcaaaccaat gccgaactgg ggtggtgatt tgtcgaaaat tgattttcaa
      361 aatatctatt attacgcaaa ggcttctgaa aagcaaggca aaagctggga tgacgtcccg
      421 gaatctgtca gaaatacctt tgagaagctt ggtattccag aagcagaaag aaagttcctt
      481 gctggtgtgg gtgcacagta cgagtccgag acagtatatc atcatttgag agaagacctt
      541 caaaaacaag gtgtaatatt tattgatacg gacacggcac tcaaagaaca gccagaactt
      601 atgaaaaagc attttggaaa agtcattcca cccgaagata ataaatttgc ggcattaaat
      661 agtgctgttt ggtcaggtgg gtcgtttatt tatgttcctc cacacatcaa gattgacctt
      721 ccacttcaag catactttag aatcaacgct gaaaatattg gtcaatttga aagaactttg
      781 ataattgctg acgaaggtgc tgaggtccat tatatcgaag gctgtactgc tccagtttac
      841 actacggaat cgctgcattc tgcagttgta gagttaattg caaagaaagg agccaagata
      901 agatacacaa cgatccaaaa ctggagtaaa gatgtttaca acttggtaac aaagagagcc
      961 tatgcgtatg agaatgccac cgttgaatgg attgatggaa acctcggtag cagattaacc
     1021 atgaaatacc ctggcgtgta tatgcttggg aagaatgctc atgcagaagt catatcagtg
     1081 gctttcgctg gttctggtca acatcaagat gctggtgcaa aagccgtaca cttggctcca
     1141 aacacaactt ccagaattac aagcaagtca gttagcaagg attctggtag aacaacctac
     1201 agaggcctgc ttcatgtagc aaagggtgct acaggagtaa agtcaaatgt cagatgcgat
     1261 gcattattgt tagacgagaa ctcgaggact gatacatatc catatgttga agtaaatgaa
     1321 gacgatgcca caatctctca tgaagcaact gtaggaaaga tcggagagga tcaaatcttt
     1381 tatttaatga gcagaggatt ttcagaatct gatgcccttt ctctgatcgt tggtggcttt
     1441 atggagccgt tcactaaaga attgccaatg gaatatgcag ttgagctaaa taaacttgta
     1501 aaggttgaaa tggaaggctc cgtcggttag agagaaccac ttcgcttctt tttttctcaa
     1561 aggcgctgaa tgaaccggtg attttatctt ctattaatag tgattatatc aatactataa
     1621 cagaggaaaa tagagagccc gcctggctaa gcgaaataag aagagaggcg ttttcgaagt
     1681 acaccgctct cccagctgag gtctctccgc tttattcaaa atattctgat gtgaaacgtc
     1741 ttagagcaga aagtgtcagg ctatctgacc aaacgactag atttcaacca tacgaagagc
     1801 tacgtgaaag gttgaaggaa tcagagcaag gtgctagtgt tttacgaata ggatcttcga
     1861 taaataatat ggtgatccca gaaaaaattt ctcaaatggg agttattatt ttggacctaa
     1921 aagaagcagt gataaaacac gaggatctta tcagaagtca gctaacctcc aatcgcctcg
     1981 agtatagtga agacaagttc ctcgcgttag aagtgtcagc tttcaattcg ggtctgttta
     2041 tctatattcc caaaaatgtc gtacttgagg atccaattag aattattaac tctttggcag
     2101 atgatggatc ttcttcgata gcgcgggcta ttattattgc ggatgttggc tctaaggcta
     2161 ctgttgtcga agagatatat gctccgcaga tggaacggga tgagacagtt caacaggcat
     2221 attttgagtt gatagaatgt catgtcggcc ccaacgccca tctagaaatg gtaaccttac
     2281 aagctatgag cagaaacact attaatttct ccaataggaa ggccttcatt gagagagatg
     2341 gcaaaatgtc gtggtatctt ggaatgtttg gtgcagaaat gtcaagatac aaggttgaca
     2401 gcataatgaa aggtgtcgga gcctctgcag aggatgttga aattatattt ggaattggga
     2461 atcagtcatt cgacgtgacg tcgaatctga ttcattatgg aaaaaattca aggggtagag
     2521 tactcgtcaa atccgttatg aaagatactt caaagtcctt gtttaaaggc atgataaaaa
     2581 taggcaagga tgcgaaggct tccgagtcat atctagcggg gcacgcaatc ctactcgata
     2641 agggtgcaaa atcagatgca attccaggcc ttgagattga aacaaatgag gtaaaagcta
     2701 cacattctgc atcagtagcg cagatggacg aaaaccaaat tttctatcta atgtccagag
     2761 gactaaatag agaaggtgca aagcgcgaaa ttgttagtgg ctttcttgaa ccattatcaa
     2821 ggaaaatggg ccctgctatt agagcatgga ttaattactt gattgaaaac aaatgggctg
     2881 gcaaatccct catgcttaaa acagacgaag caatggaaca gattcttgaa gtggaaaagt
     2941 ctagataccg agagacacag gatatttttg agaagcacta taagtacaga taggtcttga
     3001 tattagtggc acccgaatgg ataaaggcct gtgaagtgaa agaattgaac aattccgagc
     3061 tcgtaggttt tgattttaat gaaaaaaaaa tactgatatc aaagatacgc aataaggttt
     3121 atgctacaga cagaatctgc acccatgcat atgccgattt gtcgacaggg ttcatgaatg
     3181 aggacgaaga aactattact tgtcctttgc atatgtcttc ttttaaactg gaggatggca
     3241 ctccacaaaa tctccctgca gaagtgcctt tgaaaactta caaaaccaaa atacaggata
     3301 cctcggtcta tatcttagta gaataaaggg attaaaaaca gcatcgactt tgttgaacga
     3361 attagtatct tcttgaatat gttaacaagg cttgttacct ggaacttaga aacttgatct
     3421 tatgctcttc acagcaaaaa tgttctatcc ttgaaacacc tctgttagaa tctccttcta
     3481 tatagggcca tccaccattc gaaaactcct taccgcaata cgcacaactt ttgtcctcag
     3541 agcgttcatt cgacatccat tattattatt tggaaaattc tattaaaact tgctaacatc
     3601 gtaactggcc aacgtacccg attcgagcac atgcactcaa tcaagcatct tttaaggcat
     3661 tttataaaga atagtctaaa tactcttgca aaaatctcta aaatatggta gtcctacaga
     3721 tgatatgtga taagtgtcaa aaggttatct tggaaaaaga aggagaatct tatcttgacg
     3781 atggccggtt tccgatttcc gaggacgagg ctgctatgct agataaggaa cacagaggtc
     3841 acgaatgtca tattgaagct gtaaaaaaag gaacgtaaat tcagtccgat attttacaaa
     3901 ttatatgtaa atttcttata gaagattaga cgtggtctat tttccgaata gaggacggaa
     3961 acaaataaat ttcataccta gcctttgggg tttaatgcat aaaaatacgt acgcaaatgt
     4021 tccgacactg tcggcaattg caatttcagc aatactgctt acatccttca ctatggtagg
     4081 gatgcagtta tcacaacatg cgttggctca aaatacaacg agcgcaacac caac
//
LOCUS       DAOR01000077            4517 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_48471, whole genome shotgun sequence.
ACCESSION   DAOR01000077 DAOR01000000
VERSION     DAOR01000077.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_48471"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2414..2646
                     /estimated_length=233
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1286 a          842 c          777 g         1379 t
ORIGIN      
        1 gcttaatgtt gtggcttaga ttctattatt cagaaaggaa tggtaaaaag atgattgaag
       61 atgaggatgc taaattaata tgaaagcaac ggcaaataaa ccaaatcaat acttcaaata
      121 gtttacagta ccgaaaataa ctttggacga gccccaaagc aacttatgta ttcatctttt
      181 agttgttatt gccatctctg ttcattttgt atcgacaacc gttcaacaat tcgatgttat
      241 tttcatattt gtagcaaata tctctgctcc tccattatgg agagagagag ggcggcggtg
      301 gtgtaatagg ggttaggaag ataagccacg ctactacaat atcctaaaaa tactatcaat
      361 gattatttct tatcattgta aattattgtg accacttttg atatatagga tcaaacatcc
      421 tgtacatttg agagcacatg ttttctgccg tcattatgac taggtgattt aattattgac
      481 ttctagtcca aaaatattag caattgatcg ttacatctca aatctcgtca actccacaag
      541 cattgactct agagtcggaa cgttagcaac tgaaatagat cacaagtcta acaatcatct
      601 ttcttgtcga tggcaagtct cccaatgcac tcgctgcagc ttacctatac ttcgcagcaa
      661 tattgttagg agtcaaccta cccgtgtcaa gtcttgctgg gtgggtgatt atttgtctgg
      721 agttcttatt ctattcagtc accgtctctg tataaacaac aatacacgag tacgagtcag
      781 gcgatgcttg ggtagactta tctatcagtt ttatatcatg tatatccttg gcttgacgct
      841 gcatcagaaa ttcgtttacc tgtgttacta tgttgtatct tgatccataa aatatctcaa
      901 tctgtttatg ttcatgtgtc tttttagctt ccttcacagt catttgtata caccaccgcc
      961 aacaccaatc gtcatttatg tgtaatgcga tggcttcaaa tgatggtttt acttatacca
     1021 taaccacttc gatcatctat ctgtaaaaaa ctcattttcg ttaagattga attcagtctc
     1081 atactttccc gaaattcatt catattttaa tttgacttct ataacccctg ttagctgatc
     1141 tatcgtgttg caatggctga ccattttgga caagccaatt atagtcctcc ttgctgtgct
     1201 ctatatcacg tatcatttcc gtctgtttgc tatttttgat tcctttgata gaatactcta
     1261 catgaatgtt aaacgctgct cctatgccta cgttccttag tcgcaagcgc acatcaaatg
     1321 gttctacatt gttaattgta gatgcaacgc gcaaatgaga aaaagctgga cgaagttgat
     1381 tttttgttaa aaatctggtt tgtttggcat aataggcagt aattatagct agggcgatgg
     1441 tcgaaattgc taatataaat ggagaataat tgtcaaaaaa ttttgtaatc tgatacaagt
     1501 caatcatgta caaaaatacc cagatgccaa tagtggtgaa agtcactggc ttagtcatgc
     1561 agtagtcact ctgcggcagt ggttgactgc aacataccgt gatacagttc ttcaaagatg
     1621 agaagcccat tttttgcgtc cataggctgc ttttcattac gttcttttat taaagttgaa
     1681 atcacaactc tggccatatt ataataccca acaattccag accgtacatc aaggtttttt
     1741 tgctggagac tatataaagg gtaacgggtt cctcatattc ttctttaatc cacagtattt
     1801 caaagcctcc tgcttcaacc aaatcatgta tttccttttc tgtaaagaat ctgatttgaa
     1861 agccgttgat atcatagatt cctttgtcta tttctattcc tttgccatag gaattatcat
     1921 tctggtttct gacagaaaag aagttcaaac cttttggttt tagtactcta cttatttctg
     1981 aaaatataaa atgaagctcg tcaagtgata atcgcatgtt gagcagcatg tgcgaatata
     2041 cagcatcgaa atagccatct ggaaatggaa gcgctctcct aacatcaaag attcgtggtt
     2101 ttattggcag tcttttttcc tttgtaatct tatccagtaa ttcaatagtg acaatagaat
     2161 aatctactgc ctgtacttca attccatttg atgcaaaaaa tgttgtatct cttccatgac
     2221 ccgcgcctag ctccaatact ttctgtatat tgttggtttt catatgatta atgcaaagca
     2281 aggcaaagtt gcttggttcc tctccaaaga aagtactgtc cgatttgtag atgttattcc
     2341 aaacttcact cattattgct atccttcttt tttattgcca tagtctttcc aagcagaata
     2401 gcctccttca atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnncaag cagaatagcc tccttcaata gttacggtat caaatccttg cttgtttaat
     2701 tcatctgctg caatattccc tctatatccg ctgttgcagt aagttataat tttctttctc
     2761 ttaaggtcat ctatatcccc atgtcttgcc tttcttatta gctgtccaag tggaatattg
     2821 actgctccat ctatcttgcc ttcttccaat tcatcagctt ccctcacatc taatatcgtg
     2881 tattcgtctt ttccttcttg cagttctttt gaggttactt tggttgccat tgggtgatga
     2941 catcgtaatc accatatcta atttattgat tgagtatcgg gaatttttat gacctgctgt
     3001 agattacatt gcagttggtc aatttcagga ctcagccttt taaaacaata tgtccgcatt
     3061 gctggtctaa tatatccaaa aaacgaaact gctgagtgaa atacgaaaaa ctataacttg
     3121 gtgaaagatg tcaataaaag tttgccaaag aaatgactct ctcagatatc aacgtaatcg
     3181 taccaatcgt agcgggaata atcactgttg gtgcaacgac aattggttta gcttatagaa
     3241 taggaaaaaa gtctggtgaa atacaacaaa agctcaaagg tctatcaaaa gaggtgaagg
     3301 aagacaagga acgcataacc tatatagaaa ggatttttat gaacaatagc cccgtaatag
     3361 cgggaggtat gttaaagagg ttctctctga cagagggtga gaaaaaagaa tgagttacaa
     3421 cacactccca attgatctgg ataaggaaaa gcttcggtac ttgctagaaa agctttcaaa
     3481 cagaactcta caaagagaag aggcgataga gctaaaacca ctagttgaaa gaatatggaa
     3541 ggatgccata aaacaaggtg acccagattt tgcctcagag gtagctcaaa tgttaattgc
     3601 attagacggt tacatacatg gaaggattga gcttgttgag aacgtgccaa taagtgaaaa
     3661 attggatacc aagacgactc ggtgggaata ataacagaag ttcattcttc caatccgacg
     3721 tctgatgtta taccgacgct taatcgagtc ctacaaggca aagaaatgaa gcataccaaa
     3781 tatcgaaaac cgttagaaca atttgatagg tacgattagg atgccagtaa atgatcgctg
     3841 aaacagttat tttcattgtt ttatgcaata gcaattacta cattctcagt attgccgtca
     3901 cgtaacatat tggaaaaccc cgttcgcagc gtccggttag actgctacta cgaaaaactg
     3961 gacgttgcct attaatatta gactaatagc ttttaccttt aggcgatatc ctactcattc
     4021 ctttcaagat cttcagatag tatcgaagtt caccatagga agccatttag cctgaaaagc
     4081 tctctatttc tgatagatgg actcttatca atctgtttta gaattaaggc cttactacca
     4141 taccttgaat cagcctaatc aataggctct gttttcaact ccattacttt gctaccccct
     4201 gtagttgctg cccaggtaag aatgactcgg aacggattgt aagagccaaa gtgccctcat
     4261 catcaagtag gtcagtgatg ttatcttcga ttttgcactc gccttgatag aacaggcctg
     4321 cggtcttttc gtgggcatgg aggtagtgac tcgcagttgg acaatatcgg taaagaaagt
     4381 aacagaaaga tattggaccc attaggagtt tagaagctta actttcatga aattgaacaa
     4441 acgaccagcc tactatttgt agtacttaca tagatgggtg acactttcaa atacccacat
     4501 aggtgacact tttctgc
//
LOCUS       DAOR01000078            5157 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_76194, whole genome shotgun sequence.
ACCESSION   DAOR01000078 DAOR01000000
VERSION     DAOR01000078.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_76194"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1790 a          866 c          972 g         1529 t
ORIGIN      
        1 gcaacattac aacaggatcg tatggatgta cagtctcata ctatggatca taaaccactc
       61 gataagatgt caacgaatgc tttaatatat gaaatagctg gttctaagca atgtcttgct
      121 agccatggca ttgattcaac gatcttcggg tatcctttca atctaggatc gaataagccg
      181 tccgttgttg atattgtatc taaatattac aattttgcca ggaccggtac atatcctctt
      241 atgtcgttaa actgtaatgg atttgctaat ataaagccac aacaaacgga ttgtagaaca
      301 tatttacctg atggtaaatt aacttttgca aatagatatg atatcagaag tgacagcttt
      361 ttccatataa gcagcggtca caattatggc ccttctgaaa tgtttcaaaa atttattcaa
      421 caggttaaca gtcaggtgcc atataataat aatggcaaaa taaatgctat tcctataatt
      481 acttatcatg accttactta caatatacaa gattacaata aggccggtac cacaattact
      541 gtcccgttgt ttgatcaaga aatgcaatac ctacatgata atggatttaa ggtattattg
      601 ttaaatcaat tcggatttga tccaactaat aatgtttttt atttaaagaa agttccatcc
      661 tctacttctg gaactgcaac taatgccgcc actttactaa gtggagctcc atcttagtct
      721 accgctcata gtaaataatg aagtggatgt gatgtgatgc attcttctcc accaaaggac
      781 ctaattattt tcagggatgc gataaccctt tgcaactata atcttcataa cgcaataaaa
      841 caacggatga ttcaacagaa cagaaaatgg tagtaaggat ttttaacttt acaaaatatt
      901 ttgcactttc ctttatcata tagtatgcat cgctctcaat tattattatg ccgcatattg
      961 ctcaacaaac aatgggtcag acaatgacaa ctacgccaat ggcttacgac tttaccaata
     1021 caaactctac aacaagcaaa tccttcttga cataccaaaa taattcaact ttaggaataa
     1081 aagtacaata tccatctaac tggaaaaacc aacaagttga tggtgtaggt gttgtattta
     1141 tttcgcctcc agaaactaat ttagatagat ttttggaaaa attaactata gctttttttc
     1201 ttcaattaat aatacctcca taaatgaatt agcgaatcta gcaatcaata attataaaga
     1261 gcattataca ggttttcagc ttattgaatc aaaggcaatt accttcaaca gtaatcctgc
     1321 gtacacgata gtatattcat atacagatca ggaattaaga aaggttatag caacggatgt
     1381 cgcaacggct aaggacaata gagtctatgt tttttcatat tccgcagacc caaaagaata
     1441 ttctagttat atgccaacaa tagataagat gattaattcg tttaagacaa ttcctataaa
     1501 cctcacatat tctaacaaag tttaggatct taaaaaaact tgattcaata attaactaat
     1561 atacaaacac cattcaactt ctcacttcgg aatgaaagga attgatgttg gccgggttgg
     1621 aaagtagtgg taacagctag tagtatgaat aacggagatc ttctatttat gagactatat
     1681 taggattaga tctaattcat cggcagaaga cgcagctaat ttacctgttg agaacatata
     1741 atgttggttg tggttctctc gattatgaat ttctggagtt cctataatga gatttagtca
     1801 gacgcagtga aataatctag attctgaagt aattgatagt tggaaaaggt gataaattgt
     1861 ggtacacttc tataaatgtg ggtaatacga tgagtataac tatcggatgc taaaaattga
     1921 caattaggta actgatcctc agctattata tacgataaga ttattttcat ctcagaggat
     1981 tgaaaatgaa tacaaaaaca aggatgttca ctgtatttgc ttattctagc agcaacgctt
     2041 gcagctcaaa catactctca aaaatcgata tcgagcacgt tcaggatttg ttacaagcaa
     2101 gaaagttctc acgtcataat ggtgaaataa tttgagtaac acaaagggac cacaaataac
     2161 aaggcaaaaa atagttgtat attgtgtagt aggtcttatg attagtacag ctgctatata
     2221 tttagaacta ggagcaaggg gtctagtgag atcgtctcat tttaatgaat caggtgttgt
     2281 gcggcaccat atcgagagta atgccagaga aagtggtggt cctaggatac caggaggcca
     2341 aaatatattt ttcataatag ttggtatagc ctctattata gtggctgtat ggatgctgaa
     2401 gaataagcgc aatagtaaaa taccatacat catagctgct gtgggatcct tgggtataat
     2461 cattctgtat ataattacta gaactgtaaa tattccatac cttggtttag aagctgaagt
     2521 gaatacaata gatatccttt ccaaggtatt acaagttgga ataatcatag gctctgcaat
     2581 tgtgataaga caacaacaaa aagaacaaca actaaagttg tatacgtaaa caagtttaga
     2641 tgtctaaaca taaggtcggg ttccaaaaag taaggatgtt gatatgcctg cagagggggt
     2701 ggttgatgct aaaatattaa tcttaaaaca aattggcgaa actccaggaa taggacacag
     2761 ggaaattcta agattaactg gtttgagcaa tggcgcactc gaatatcatc tgagaatatt
     2821 ggaaaacact cataaagtaa atgtcgacag atcgggcggt aggagaggaa gatactatcc
     2881 tgtcgatatt ctagcagatg aatcacacat actaggattt atcagaaata atgtatcaag
     2941 acaaataatc acttttatac ttcaacataa actgtgtact tttaacgaaa ttcttgagca
     3001 aactaagaag gcgccatcta cactatcttg gcatctaaag agactttcta gagctacaat
     3061 aatatcggtt acctatggaa aggagcacca gctttatcaa gttttaaaca ataaatttgt
     3121 acaattcatt atacaaatat ggagagagct ttaaagaaca agttgcaaac gagatattcg
     3181 aaaggcttta acactatgaa agatattgga tttgtcaaag acatacactt taagtttcaa
     3241 caatatattc tggatcatcc gccagaaaat attcactcca gcaaggtttc atgatttaaa
     3301 atgcaaaaca tctatatttt cttaggaatt accccgatag ctaataaacc actagaaggc
     3361 gctagctaac gaagtatcga agcagagagg atattctctc aatcattttg aatgttgtta
     3421 gtgaaggagg agcaactcag acagatatta tgtataaagc ttcaatgaac ttccgactaa
     3481 tgaaaaaata catgtcattt ctgtcgaaga atggtctact cataaaggaa ggggcaagtc
     3541 atagaataac agataagggt gtgtattttt tgcaaatata tgaccagtta aatgaaatga
     3601 tataaagaat atcaaaatga tagaaaggtc taacgagtgc cgattgaact ggcatgagtt
     3661 gtgggatact tatctctaat gatgtggaag tcccaatatc agtatccgat ttaggttatt
     3721 cccatatgat atcagtcact ggtacggcaa atcttgaagg aacgttgcac aacctatgct
     3781 attctgctgg gccctcccgc agggggtcat ggacattcaa gtcatatttt ggtctacgat
     3841 ggaaagcgat gacaattgta tgtacttatg ctactacatg gtgtagtaag tacgaatcca
     3901 gctcttgcat aagttctggt gaagcatagc attacgggtg gtaatgccag cgctcccctt
     3961 ggatttggta ttaaccatca aaagcacgag cagcagcaat cactagtaac atgatgataa
     4021 aatgtgcaga agttagtgtg aggctactaa gattttattc atacttcctg atacatttct
     4081 tctgttctgc tatttggaat tagtttcgtt ttgtggcacc acatttcagt ccccaaggtg
     4141 tataaaggaa gaaataaaat atctgtcttg accactatta cgatctttct aaatatttac
     4201 aattgaagaa taggatcgta gtgtcagtga gcaggccaaa aaatacttgg ctaactaacg
     4261 ccttctagta ctgtggttat gggtgtaaaa gaaattatgc tggttctgaa atggctgcgg
     4321 agccaatatg actaccaata agtaaagaga aggaagaaaa gtcagaagta gggagtagag
     4381 ataatctatt atacaataga cgcaataaaa tcacagataa tactacggtt atcatgacat
     4441 cttctaccag cggctgcatg agattcatag aagatagata aatgactata aaatttatgc
     4501 gataattgct aatttattgt gtttaatgca aatacaattc atatattgac gcctgactgt
     4561 taatatatct aacagttgta gaatactcaa gatatgaata caaaaacaac aaagaatgta
     4621 gttttgttga taataggtgc ggtcatatta gttagtatca acttatcaac agcgcctatt
     4681 gcatccatac aagaagcaaa cgctcaggcg aacagcaaca tgactggcag tattgttggt
     4741 agtagtagtg gccaaaatca aactatgggc tctaatatga ttatagacta tcctggggga
     4801 tcaaatatca ccggttctat accaattgta tctacaatat ctaaatcaac agcatcacaa
     4861 atacacatta gtcttccgaa tgctactgcg actgcagaaa agtctgtcgg tactaatgca
     4921 catgcagtat tagctcgcct tgggatagta catggatttc tagtatatac agtattggta
     4981 acagatagcg actctaactt ccattacgtt acagtagatg ttggaaatgg caaagtcctg
     5041 tcgtctcatc aactatccat gatggatatg attcgtgatg gtgcagtgat gacggctatg
     5101 aggccaacaa tgatgatgat tcctggaatg gggatgatga tgggccctgt aatgatg
//
LOCUS       DAOR01000079            4254 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_48476, whole genome shotgun sequence.
ACCESSION   DAOR01000079 DAOR01000000
VERSION     DAOR01000079.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_48476"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1376 a          788 c          786 g         1304 t
ORIGIN      
        1 ctttcagtaa gacaatgact ggtccatttt tcattcaatt ataaccaact taacaattta
       61 tacaggcttg cttatacagg gaaataatta cgattgataa agcaattaca tagtatcttg
      121 tgatacaaat tgacgtaagt taagtctctc gtaaatactt aattcggagt tcatttcata
      181 caatatgaaa aatagaagta gaagcgaaat catcaaatca attctagagg cggccaatgt
      241 gggcgaggga gcaagcagga ccagattaat gtacaaatcg tatctagcat ataatcagct
      301 gaaagaatac ttggaaattt tgcaggaaag cgccctaata gactacgaag taggaatgcg
      361 gtgttacaga ataaccgaga aaggtacaag attattgcag ttgcaaaata aattggaaga
      421 aatcgctcct attaactatg tcaacataaa aaatagttga aacaccagac agatagtagc
      481 tgacggatct gtgacgccag ttttcttttc taatgattga agaggaaaac tgttattgaa
      541 tctatgctct attcctgata cgctgaatcc attttatgtc agaatgtaac tataattttt
      601 cttatgaata taatagtcgt tgaatatatc gacaatttct ccaacagtct tagcacttgt
      661 tgtgaagcac tgcaaatata gatagttgac gatagcctct gagaaatgaa ccacaacgta
      721 actttaggaa tcgtcgcaat cgtcgcagct atgaccctga ctgctgttgc gtttgctata
      781 ccacaacagg ctttggcgta caggcaccac aatcacaaca atagcataaa agttgatcaa
      841 cagatcaatc aactcaataa ttgtactaga gcgttatgcg ataaccaggc caataataat
      901 ttcggatcac cgcctctagt ttggcagtag taagaactca attgaaaagg tcaatcagca
      961 aaagaactac actggcccac gtgtactacc tcgatgggac acctctacag attgccagta
     1021 ctttggcgaa aacccggctg acataagcaa ataatcttga aaagtgcaat tgaaggaaag
     1081 taagccttca tcattatttt tggtcagtac aacgacacta cgtatcgagc tactaccgcg
     1141 tagtgcgata aaacgtatgc ttgcggccct attaaggcga aaatccggat taaagcctcc
     1201 aatattaata atttattcac atatttcttc tcagcttctc ccacaaatcc gtttaagttt
     1261 ccgtcgtgat caacagcata acacggttcg actgtttttt gctgtgaatc gacaaaatat
     1321 actaatagcc tctaattttt tgataatgat aaaagatact cttgctcttc ccaccaccag
     1381 ttgctacatc ccatgcaatg cgatatatga cgctgggatt ccaactatgg tatgctagtg
     1441 ttacaccggt aataggctat ggctaataat ttgtagaaag taagatttct ttgatttgtg
     1501 gattgcctat catgaggtct attattttca tacgttcttc cctgggcacc ttatcatcat
     1561 atgctactcc aagagaatcg attgctttaa gtttccaata gttgttgact gcacttccga
     1621 gtaatctctg agcatcatag accaccctac caaatgcggt aacaaaatat cttccctttt
     1681 gtctgtttac taaaccagct tttgtgagac gagacattct tgaatagtat tgctttttgg
     1741 taagtttgaa ttgggttctg agattctcac cagtatctcc actatctcca ctagcaagaa
     1801 aaatagtctt gaagataagc agtgatttgt tatcggatat taattttata acctccctta
     1861 agaattctaa cttctggttc cttcctgcgt catccaatct tgcagagagc tcaatgtcag
     1921 aatttgcgtt caaacgatct cagattcctt tgattatgat atgcatggta ttaattaata
     1981 tctggataat cacagactga aataccacaa acatcttttt atgagcttag gataatagct
     2041 agagaatcag gttgtgccaa aacgatcttg aataattatt caatttttat ctatagaaac
     2101 agttctgccc gtagcctact gaaaattgga ttgtatctta tttcgtgctc attattctat
     2161 tctaatgaag tctagatctg ctgctctaaa ttgttacaag atgcttcacg agaacgttcc
     2221 tgcgctattg agtttggagg ccgtaggatg agttggtacc ataccaagtg ctaacacaag
     2281 tgaaatacaa agaaatgaaa atatgatact cctgttgagc attaaagacc ttctgagcgc
     2341 ccatcctatt aaaaatttgt tcacaaacac ttgttcgaat ccactacagg ttaaggggtg
     2401 aatctcatgt atagacagtt tcacaggtta tattcaagaa ttagcaatag aatagaatga
     2461 aacagatctt tacagtaggt cattccaatc gttcatggag tgactttgtt tccattctcc
     2521 ttaaaaattg tataacaatg gttgtagatg tacgccgttt tcctggatcg agaagatacc
     2581 ctcattttaa caaagcaaat atgataaagt tgcttacaca tgaaaacatt gaatatattc
     2641 atatcgaaaa actaggtgga agacgggaga aaactgatct ggctcacaat agcaatagtc
     2701 attggcaaaa caagagcttc caagcttatg ctaattacat gaaaactcaa tcatttaaag
     2761 aaggcattga tgagatactc ttagttgcaa agcataatac tatagctatc atgtgtgctg
     2821 aagctttgcc gtggcgttgt catcgcagat ttataagcga ttatcttacg atgctcgggc
     2881 tggaggttca tgatattctg aacactaagg aacctaacca tcacgaattt acttcatttg
     2941 cgcaaatcat tgacggagag atcagatact agaaagcgca gatattggaa ttatctgttt
     3001 actgactatg gcatgtaaca aaaattgagt atgacgttat cggaaaaata tccttgttat
     3061 cataatattc gaagtgccgc catagatcaa ttccagtacc actattttct ggcagtttga
     3121 atcaatgaac atatcataca tttttgcaat atcatctatc cctcttattt tgaaaaaaac
     3181 caacctcgaa attaatgatt tggcttgccc caggtcctac aatagctaaa gccatcgcat
     3241 cgcaagttca tgtcagtcta gataatgcta gtatcattgc agaaaaaaca gtgggtgcta
     3301 atgcacacgt agctgcagtc agaattgccg tggttcatgg atttctagta tacatggcat
     3361 tcgttgtaga tagtaataat aacttccatg gtgtgctagt ggactctgga aacggaaagg
     3421 ttctatcttc tactcaagta tctatggtag cattgatgcg tggcggtatg ggaatgatgg
     3481 gatctggaat gcaccaaggt gtgatgacgc attatcctgg aatgatgagt aaacctcatc
     3541 cttaggagga aagctgcgtt tcctttcctt ttgtaaagac ggcctaagct atactagcaa
     3601 tgacaacgct gctcaacaat atgcttttct ttgaattcgt cagcaaatac tatttgtcat
     3661 tagataataa tgacgatgac aaattacact tgtgagttcc atattcaaca tttgtatcta
     3721 cttttatatg acacttgtat gacttttgac agttgaatag gagtctgccg aatcaattaa
     3781 ggtaaaaata caaaattatg ggctgtgtat ccgaatgtgg aagccttaag gaattgtcca
     3841 tgtttaggtg acagattgtg tatctatatc tcttaacgcg atatcgcggg ctcgtagaat
     3901 ctattaaatt taacgtactt tttattgttc aagattattt gctgtcaaac aaagcatcga
     3961 catgataatt aaaggcgaga gattaaaact tgccatcctt tctgtattgg atgatatcga
     4021 actacaaaag attttagatg cggcaatgta tcaatccaaa tcagctaatc aaattatact
     4081 tgaaactaac atctctcata ctactgctta taaaaagatc cgatggcttg ttgaagagaa
     4141 gttgctcatc attgataaaa ttgagattac agaggatggt aagaaatcaa gtctttttcg
     4201 tacaatatta aaatcattca gtgtaaaata tgaatacaac aatgtagtta ttga
//
LOCUS       DAOR01000080           15638 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29560, whole genome shotgun sequence.
ACCESSION   DAOR01000080 DAOR01000000
VERSION     DAOR01000080.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15638)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15638)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15638
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29560"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1168..1368
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7739..7977
                     /estimated_length=239
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5009 a         2873 c         2970 g         4346 t
ORIGIN      
        1 gaaatcgctc ctattaacta tgtcaataaa aatagttgaa accctatcta gtaactcaga
       61 cgtagaaggc ccgctttatt tttcattgac acttcgaata tcctatatat attgaatatg
      121 aaaatcattg tcaaatccat gatttattta tgaccgataa atggaatctc cagtgatact
      181 tttactaaaa gtatcacgcc cgaaagcaac tataattttt cttataaata tattagtcgt
      241 tgaatatatc tacaaattct cctatagtgt taccagttgt tgtgaagcag tccaaatata
      301 gtataatgta agatagcctc tgagaaatga accacaacgt aactttagca atcgtagcaa
      361 tcgtagcagc tacggcactg accgcaatac cattcgccgt accacaacaa gcattggcag
      421 gcggaaacca ctacaaacac aacaatcaca acaatcacaa cagcaatagc atcaaagttg
      481 atcaacaggt caatcagcaa aatgcatgca catcaccacc accacaaaaa atggatacca
      541 gcagtttaaa cggtgcgatg ggtagcagta gtagtacagt ctgccttaac tttggcgaca
      601 atagtgccga catacacaaa taatcttgaa tgaaagtaag acgaatcttc tctttttttt
      661 tctaagagca caacgacagg atgtatgcgt ctaattttaa ttgcaaatca acaaaagtct
      721 gaaacgagaa gacggtataa cacgctaatg ggatagaaca tttaacaatg aatccaagcc
      781 agctgataca agccatgtct ggacgcccat tgtccaaaga atggctcagg gtaagacgga
      841 tgaacagact gaaccaagaa ttttatgatt tgcacggagg tcaatgaaat acattcacgc
      901 ccgagaaaga ataatattct attctattcc gaaattatga acacctaaaa aggacgagat
      961 tcattacttg agcaacgaaa tgaacattct gtccgacgat ggtattcagc gtaacgaaaa
     1021 tgaatcatcc gacgttaacc acacagaacg tgtctcgcaa tgctgggtct tttgactgtt
     1081 aaagtctctg acatagcaca gcaattgtct aaaatgaacc ctgatggaga gaagtaatgc
     1141 aatgtcttca ttgcaagaat accataannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc cgataatttg
     1381 agatttgtgg tacaaaaaca tcatgcaacg agattacact acgactttag attggaagca
     1441 aaagatggca cactaaaatc atgggctgta ccgaaaggta tctcacttga tccaaaaaca
     1501 aagaggctag caattatgac cgaagatcac cccttagatt atttattatt tgaaggtgtg
     1561 attcctgaag gaaactatgg agcaggtact gtaattgtgt gggatacagg gacttatatt
     1621 acagagcgag aactttcaga gcagctacaa accgggaaga tatcttttat tttatttggc
     1681 caaaaattaa aaggaagatt tgcactagtc aagattaaaa gaaaaaaaga atcaacagag
     1741 gaagaagaca atcaatggct tttgataaaa gaaaatgatg aatatgcttc gcctgttcta
     1801 gatctgagta atacaagacc cgagtcagta ttaactaaaa gaacaattga agatttggaa
     1861 gccaaaaaat tagaaagaac caatcgtaac caactagaac aaaaaagaag gacaaggagc
     1921 aacaaagagg aaatagacgc aaacgacgaa gagacaggag aaaaagaagt caagccaaac
     1981 atcacatctc attatgagtt tccaactact gttaagccaa tgctggccac attagtagat
     2041 aagccattta ataacaaaga ttgggtcttt gaaataaagt gggatggagt ccgtgctctg
     2101 gtttttctta gcaagacaag acaaattttc gaactaaagt ccagaaatga taaatctatt
     2161 actcacagat acccggaatt gttatcacca ttggaggctg cgataaactg cagagaatca
     2221 gtgattcttg atggagaaat agttgtggtg gacgataaag gctatcctaa ctttcaaaac
     2281 catcaaagaa gaatgaatgt cgactctcct aaggagattg cgacgctggc cgaacagata
     2341 ccagccacgt attatctgtt tgatatcctg tatcttgatg gaaagaatct acagagctta
     2401 ccgtttgtag atagaagaaa aatcttatct gatgtgatta gaccaaacga caaaatcaaa
     2461 atatcagatt ttgttgaaga gaagggactc gaggtatata aaaaaatcaa aactatgaat
     2521 ctagaaggaa tcatggcaaa gaggaaatcc agtatgtata tgcaaggaac aagatcgact
     2581 gattggctaa agatgaagat tataaaaact caggattgcg tggtaatagg atatactaga
     2641 ggggaaggaa acagagcagg ttatttcggc tcacttcttt tggcgatgta tgataaggca
     2701 ggagaattag tatttgtcgg tcatgttggt agcggatttg attttagttt acttaatact
     2761 gcctataaaa gattggaaaa aatgaaggta gacaactgtc ctataaaata tgtgccatat
     2821 acaaatagag agccggtttg gataaggcca gaactcgtgg tagaggtaaa gttccatggc
     2881 tggacaaagg atatgattat gaggacaccc atattcttaa gatttagaga agataaatca
     2941 cctagtgaat gtaggattga gttggaagaa cccctagcag aagttgttcc aagtgttgac
     3001 gctactaagt atcatacttc taacatgaga aaatcaatta ataggcaacc tagtaatgta
     3061 caatcattct ctaatcttga caaaattttc tggaacaaga caaaagacca ccgagcatta
     3121 accaaaagag acttgattga ttactacgac actatgagtg gatatatact gccacatctt
     3181 aaagatagac ctatttctct aagtcgatat cctgatggag cattaggaaa gcacttttac
     3241 cataaaaatt gggataaaca aagaccagag tatgttgaaa cagtaaaagt ttattccgag
     3301 agtcgtggag gaataatcaa ctacatagta tgcaacgata aagatactct tctatggctt
     3361 gcaaatttgg gatgtatcga aatgcatcca tggtacagca gagtcaaaga tttcgattcc
     3421 tgcaaagggg cagaaataga ggaagacaag tgtggattga attaccctga tttcatagtg
     3481 tttgatttgg atccatacat ctattctgga cacgaggctg ctggtgagga gccagaatat
     3541 aataccaaag ggttcaaagc agttgttgaa gtagcttatt acctaaaaga cttacttgcc
     3601 acactaaaca taggctcgta cgtaaagacg tcaggaaaaa ctggtttaca tatttttgtt
     3661 ccaatactac cttcatatac atatgatcag accagaaaat ttgctcaagt tatagggaag
     3721 attttggtta gaagatatac tgaaaaaatt acgatggaat ggaacaccgc gaggagaaaa
     3781 ggaaaggttt tctttgatta taaccagaat tcgaaaggaa agactatagc gtcaattttt
     3841 tcggcgagac cgactccctc ggctactgta tctaagccag tacgatggga agagctatct
     3901 tctattatgc caacagattt taacatacta aacgtacctg tagctatcaa aagaactgga
     3961 gactcatgga aaaacattct agaagaaaaa caagatatta atacaattct cgaaaatgtg
     4021 aaccaaatcc aataacgatt atttcataat atagtataca agcagagatg ttccaagaca
     4081 tttcaaaatg agaactcgtg ataaatacga tatattttcg gatattctga aggcggctgg
     4141 tcggagaaat atgggatcga ctatagccga agtaactaag aaagcttcct tgaatccaga
     4201 acaagcaaaa gaggtaataa tagatatgct tgaaattggt cttcttgaat tccacaaaga
     4261 aaagacttta aaaacaacaa ctaaaggatg gcaattcgaa aggatttacg agaatttaag
     4321 cgaatttata ccaatttctt ccaagataga tcatatgtct gtgggggcga tttagcaaga
     4381 tagccaaaga aaaacatcat tcatggatta ttgtcagctt caactaatca ttgcactgat
     4441 cgtggcggct gctacttgta ctatgagcat cttcctacga acgatgtacg aaagggcaag
     4501 aatataatgt aaacttgaca tttttggcaa catatatata ctcttttttc acttcatatc
     4561 tagcgatgag caaaagaaaa gaaatagtta tgcttgtaac cgcactttca cttgcaattg
     4621 caatagtact gcaagggtcg gtttttgaca agaccagggc tatgggagtg agcttagcaa
     4681 accccgctgc tactgtaaca cacatacaaa atgatggaaa aatgataact ggcaatgatc
     4741 caaatcagcc attaacaccg accaatcaac aatcgtcttc tgggacaatt gacacttcgt
     4801 tcaagaatat atctctacca tccactacca acgaccccac tactcaaact acaaaccttc
     4861 ctgctgtctt taagcaagtg gagaattctg ttgttcaaat cactgctaga acaccaaacc
     4921 ctaatttaca aattataaca aatggcaatc catcaacagg tcaatctaca agattaggtt
     4981 ctggattcgt atacggtaag caaggacaca taataaccaa cagtcatgta attgatggag
     5041 ctagtacagc cgatataaca tttattgacg gaaataccta tacagcgaaa gtcataggaa
     5101 aagatatctc aggcgatata gcagtattac aaattactga taatttctca tctgaaaacc
     5161 ttgttcctct tacaattgca aattcctctt ctctccaggt aggacagcaa gtaatagcaa
     5221 tcggtaatcc gtttggactt agtgatacaa tgacaactgg cattgtgagc caattggatc
     5281 gtctaatccc caatccagat acgggatttg cgataccagg aggaattcaa acagatgcac
     5341 caattaaccc aggtaattct ggtggaccgc tattgaatac acaaggacaa gttataggaa
     5401 taaatactgc aattaattct gctactggtg gattctctgg aataggattt gctgttccat
     5461 caaatgcagt tattagagaa cttgcaacat tgactaaaac tggaaccttc aaccattcat
     5521 ggcttggaat atctggtggc aagataactc cagatttggc gcagagtgca ggtttaccaa
     5581 ggaactataa aggagtcgtt gttggttcag ttcaacctgg aagtcctgca gaaaaagcag
     5641 gcctacaggg agtgacgcaa aatgaccctt cgactgcacc ccatatcggt gacattctaa
     5701 caggagctga tggacatcct ttgagaagca tagatgatct cataaattac atcgacctgc
     5761 ataaatcaat tggggataac gtagtgctaa ctgtaaacag acatggccaa ataatgaatt
     5821 tgaatctagt gctacaggca cgaccgccat cgttagggaa cccaacacca caagaatcaa
     5881 ttcttccttg atacaatccc cacatttttt ttaaagaacg atatagtatc tgggatgtct
     5941 actgagagca tttgcctatg agtaattatg tcgtgaggag atatattatg atttatctag
     6001 agataaggtt ttaatcgttt tattgtgtct caatgacttg ttactgattt acaattttgc
     6061 taatcggagg acctacatac tcagcatcat tcggtaacag ctgtagtttc taccgagcac
     6121 cttatctctg aaatatttat ttcgcatcaa atttccatat gacacgcgac aattcattaa
     6181 agattaaatt tggtaattga tagattgtga atggcatact tacaatgata ctctttatga
     6241 tatgtttgat atctagtcag tagtaagctg tcagtacaat cagaaattaa taattcgcag
     6301 cccttaatct ccctcttccc gactcggtca ttcacttaat aatagaaatg gactctaaca
     6361 gaatggcaaa tgtccttaga cagaagtaaa tttcagatcg gtgttgaaaa ttctgctgaa
     6421 acccccatgc aatcaagtga ggagaggata atattcctcg ggtagagcac gaaatagagt
     6481 gtcctcgctg ctatgatatc atgatactat cttctcaatt tgataggctc tgttacttgc
     6541 gtgaagaatg taatttttcc ctttatctgt agtcagtaag ttgagctctc agttacttaa
     6601 gccgcccaag cgaaggaatt cgatcattca actccagtcc caggggagtt agcaacgtta
     6661 ctccgatttc actaccgtca gcatttgctg caaagaagct gggaatggat ctattcgctg
     6721 ctatgacaga ttaccacagc tggtaacaat gtgtatattg cctagccgaa ctgtggcata
     6781 gcatactaaa tgttattaat gattggatac attgcaacta cgtaggctgt aaaaagtcgc
     6841 tgtggtagta ttcaaaaaaa atgagagggc gtgtgtctta ttttcttttg atcagcggtg
     6901 tatatcagct tggttctggg ctgtgttgtc acataaactt gatgtacagt tattcagctg
     6961 attgatcgat tgagcgacat ctatgctgtt gctgttacgg ttattgttat ggttgtggtt
     7021 gtggtggttg tggttgtgtc cataagatgc gaaagcctgt tgtgttatcg caagtccgac
     7081 cgccgtcaat gctagtgctg ctattgctac gattggtgcg attgctaatg ccttgtggtt
     7141 catttattct cagtcaggat aatattctat gctatatctg agatgcttca catcaagtgc
     7201 taggaatata gaagaagtaa gtgatatatt tttgaccact gaaatattaa ttagaaagat
     7261 cgagtacact tcatttatat gacaaagatg acaataatac atttagcgaa cataggactg
     7321 tatgaatcaa gaggatgttt agaaataatg atcataaagt agacggataa caattcttat
     7381 attttattat taagtgatga ttaggggatg gatagagata gaagatggtt tgtttataat
     7441 atggttcaga tcatatgtac ctgtttgaca gacaatagtc gatttgacaa tactactatt
     7501 cgtgtaagga ttatggaaag taattgggtt agggtccacg ccaaagtcga aggctagtgc
     7561 tgtacgtgat gacaccgttg aaatcaatag aagcagagtc actgtcactg tcattgaaat
     7621 tgcttgattc gtattcgtat gcggtcgttt aagtctagat gattctaatg ttctatggca
     7681 tttactacat aatgtaagaa ggttttctag tagatgtttg ccgccattac ggattgagnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt
     7981 ccaattttgt attatctttt ccacattctg cgcagcgact gcagtcacgt tcgattgttt
     8041 caagtcttaa cgtcggagga agttgaatca tttcttacgt tttctatcct tctcgatctg
     8101 agtgtgtaga tcctttgcaa gcgtcttaac gtccttttct gatacagatt gattagtaac
     8161 aggttgctgt ttattcacat aatcagtgaa tgcttgatta gcaatctctg ttaatgtcgt
     8221 tctattatca agtgctatcc gtttcatttt atagagtaag tcttcatcaa tataccatgt
     8281 tgcccgtttt atcacgtatc ttgtgcgcag ttgtcatata tacgtattgt cagataaaca
     8341 tacgcttata taaacatatt atcatacgta tgatatgaca tatcaaatgt tagaaatgaa
     8401 atgcgacaga tgttcgggaa ggttgcaact gcacaaccaa tggttttatc gctgcgtttc
     8461 gtgcgcattg ctaaccagaa gggaggatcg tcaataaaac gatgggccaa cgtgtgtttg
     8521 tgttattgtg attgtggagg taacgaaatt acattgagca tgacgatgag cggagcggta
     8581 gagcagattg gtaaactcgt actagtagcg ctacgtgtct cttttggctt gttactggcg
     8641 ggatatttca tgatgccgag cctacaacaa atactgggaa tgattgcaat aatatcgatt
     8701 ccctttgtaa tggttggcgg atatttcttg tcggcattct tcccacaaaa gaaagaggta
     8761 acaacacaat gactatggat atagtatcag tattcggtcc tcccgctgca ttattctttg
     8821 gagcaggtgg aataggcttc gtttttggct acgcagtcaa aaaggtggca aaaatacttg
     8881 ctgtagtatt gggacttttc tttataggac tggcatatat ggattacaac ggctgggtaa
     8941 aagtgaattg gcaagcagta agtaattcaa caaatacagc agcaacaaac ggaatgcatc
     9001 aagtcataac aatagtaaat catacagcga tacaagtaca aaatcatgta gcaagttcgg
     9061 gagtaaatga aatcggatta ttcccaatcg ctggcgcaac tggattaatg ggaggtctga
     9121 tctacggcct acaaaagggt tagttagaag gttgatgtag agatgaccaa caaaatcagc
     9181 agactaatca aaagaatgac agaagagcca tttccaacag atggatcact ctacgattta
     9241 ggcgatggaa aagtgtatca aacagaagat ggatacacat ttcacgcagt aggagatgta
     9301 tcattgttac aaaatggttg tagcaatgga gcatgtagta ctacattaga agtacctaca
     9361 acaacaatga caaatacaaa ggaaagcaaa acagtatcag catcaaatga tattttcgcc
     9421 gatattgtgg gctattcaat cataaagcga gaatttgtaa aagcacttga atctacaagt
     9481 cctgtaggaa ttctgttaga tcccgaacat gggaaatctg tcgtgggaat agtacacgca
     9541 aatcacacat ttggtatcct gtcaaatgaa gtgtctgaga ataacaaaag agttgcaact
     9601 gacatagtta gggcggcaaa tgccctgcaa agcgcagtaa aatcaaatcc ctctcctaca
     9661 caagcagatg ttaaggctaa aatcgataat ctaaatgcca cattacagga agctattgct
     9721 gtacgcgttc caaaagttga tgttagtaat tctacaatta acggcgaagc tgctaaagat
     9781 cttgtgaatg aaacacttag acagtacgga tacgcgtttg gaatctcaag tgtcaataga
     9841 ggtaatacta gtaatatcaa tagtagtgca tctgctaccc ccggtgctgc gccgaaccga
     9901 aatactttcc tgaacatgtc tgcttttcaa agcgcccaag ccttggcttc acaggcacaa
     9961 gggatgctca ctgaaacaaa atcactcctc ccaacaaacg cgacatctgc gacaacgtca
    10021 acaatagcta aagttgctaa ccacctctcg cagttgaaga acgccataga tacaaaaagc
    10081 ccatatggca acgtggctag tttagtccag aaaactattt atccggacct cgatggagca
    10141 tttcgtttga aataaccata tagctaacga ttatattcaa tctctttttt tgcaagatgc
    10201 ttcgcaagta attagcggcc gctactatgc ccacaaacat aaaatgcatt taaaactaac
    10261 aatagtatcc gagtcatacc taacattgcg atccgagact ccctatctgt gaagggtttt
    10321 cacggatata atgtgttttc acagatataa tgctgaaaca caaaatatga caagagccag
    10381 aatcgatatc agatgcatgc tccacatgcc catttgcagt gatttccctg ttactagagt
    10441 catgtatagt agagggcttc gtagattttt ctttttctat tggactcgaa gggctgttaa
    10501 gatagatata ccaataattg taactagtac ttctaaactt aaatttaccc tgacctgata
    10561 ggctatactt tattctacac ccttaataaa taacggattc ctctagatag gtaattgcaa
    10621 gacattccga ccatagaagt cgattctgac gtacaaaagc aactgctaga gaaagaacaa
    10681 gtcctgctgc gtgtcagaca atcgagaata ggtccagggg gttcagctgt tactcctatc
    10741 tccatttata tcacaaatat gagagtaatt tatcgaaagc ccatttgggt tggactaaaa
    10801 tctgaaataa tagatgtcaa ttatcaggat attgcggata ttagattgaa gcgaggtata
    10861 attcatacgg acatattcct aaaatctcgc ttccacactg acgaaatcgc agtgaagggt
    10921 actaataccc gcattgcaga aaaggccaat gcgtttatcc aacacggaat taggggcgag
    10981 cacccaggtc aagttatgca tcgccagcca caccggactc caaattataa ctattatagc
    11041 tatagtcaag aggaaagaca aacagaagat atagagtcga agatggaccc agtagacaag
    11101 ttgacaaaat tagctgacct gaaacaaaaa ggaattataa ctaatgaaga atttcaaaga
    11161 cttaaaatta aattactcga aaaaatatag aatcgggtat tgtaagtcac caaaaataat
    11221 cgttatttca ccagtcactt ttttattggc atagctgggt ttatagtgca taataataaa
    11281 tacaatttgt aagattattt agattgtttt ccatctatat aatttccatt ttgcgaatga
    11341 tattcgcaat taaaatacac aactatgatc ctctttgact gttgcaagtc gcgagaggag
    11401 cgaaaataat cgatatagta catttcattt gtaataaata attatgctgc aatgatattg
    11461 ttgagaatac tctattagta tgccctaccc aaagtcaaat accttcctaa gttttgatcc
    11521 gaaccaaagc cgcagtatcg atcgtaatag atcacggtgt gagactgttt cttataacgt
    11581 ctctagactt ttgcaaggct cttattgagc cccgagatat cgtctcatag acaatcgtta
    11641 attgatatta tgtgatagag tcaatatgtc tacaacaaaa catgacaaag atactagtag
    11701 tacaaaactt accgataatg acacaagaaa tgtaatcaat caaacgtttg acgaggcaaa
    11761 aaataaggcc tgaaaggcaa tagacaaaga aacatcaagg gtagtagtta acggtgctaa
    11821 aatgttagaa gaagcgacat accatccaaa tctaacggca ggaagcaacc gttcgatcga
    11881 aaatagagcc tagtaaacaa gacaaaatct ctagaattag caagacacaa gctcccacgt
    11941 ctgaaaacaa catactactg catacatcat aaactgtcct atccaccttc atgcatcttc
    12001 tagcagtaac ccccatagct cagggagttt aggagtctca agcacttaaa gcggacgttg
    12061 accgcatctt gtcttaggcg ctagtagcaa aaagtcacac actatcctat tctagttatt
    12121 ctttactgct ttattactgc acgaaaatta ttactgcacg aaaacgcact tttccgttaa
    12181 caacactttc atacgctcga ccaatgtcct cgagtgagta tgtttcggca attactttga
    12241 ctttcccctt tgctacgtaa tctagtgctt cataaagata atctgcgccg tttgtgtaga
    12301 accaataagg cggttaaggt tccctatttc agcggttact acaagcgatt cggccggtaa
    12361 tattactctc tcgtaaagga cggaataagt aatcaccatc ccccagggcg ctaatcccaa
    12421 tggcatcttg ttatgtggat ttagtaaaag tcctggtaga ataataaata taagaactct
    12481 aaacaagagt tatcagaagt acaagattta ggccatctaa gtaagctata ttattctact
    12541 cgtgaagaat ttgagaatag actaaaaccg aataggaagc gatgagcgaa aaatggtgtc
    12601 tgcctaggac tagataagag atcattcatt cgattaaaac ccagcaactt tctgtattgg
    12661 cgtacagaat gacaaagatt gttagttttt cccctgacga gcgggtactt atgcaagtac
    12721 tgccgaatct aacagtcgat tgagccgaaa ttgagctatt gagagggtat gagcgtttaa
    12781 tttgaatttt cacttggttt ttgatgctaa ttatgccaac gttactactc actaagggta
    12841 gctctgataa ttccagaata catatcaggt ttagtgtcgt cttctcttat ttctacccat
    12901 gtcatccggg gaggtcaaac tgcaattttt aatacggtca tattaaaact caagaggcct
    12961 cattcgcacg ctcataggta acattaattt ttaaaagttt tcataaaaaa atacaaaaat
    13021 aacgctgccg ccctcccggg agggtacacc ggccttctaa gcaagttgta tacagataac
    13081 aaactatatg aagttggctg ctgcaaggca aatagctttg ctgaaaacta acgtttacgg
    13141 tgtagagcag ctgctatttg atgcccgggc tgaaaacagt agacttgtat gatacgatct
    13201 atgcgctgca agtggaaatt gagagattgc accgtgtaag gaagcggggc aaagtgttga
    13261 ttaatgtaaa tgaagaaata atgaaaaaat aatcctctac tgtattgtca ccctcgccgg
    13321 gagaccttaa gtcatcgtgt cgcaatcaat agtattcgcg agcgagggaa tcactaacga
    13381 caacagtgta ttcgcataag agaatcaaat tggcatttag ctcaattgga ctaaaattca
    13441 atacagaaat attctgaata cgcgaattaa aaatacacgt aaagcaaaaa tggaaaatat
    13501 gtgtgagttt gcaaaaaaca ttactgagct aaacaaaaag ttaaacaatt taaaacataa
    13561 caatgaagca agagcaagga ttgttgaaga tattcatgat gaagaatata caataaatga
    13621 attacttttg acatccatgg cataaccgaa taggaacgac agattataga agtaattatc
    13681 attaacagat acttgaatgt tcctgatttt gacatggttg caactgcatc tttggaggaa
    13741 attatttgcc tcatattact tcgcatcccg atctgtgctt ttcataataa tatcgattat
    13801 ttgcattgaa aatatgacca tcacagaaag catcaccgca tatttcacat accgcagggg
    13861 tcggaccctc aatgtttttt gagggatgct ttgggcagaa gtggacctca tcaacaatga
    13921 atttctttga tgcagtcaac acttttctat cataggtatg ttcacctggt ttgatagtaa
    13981 taatccatcc acgcgaaccc acaagggtga ccgaaaaaga catatgggaa tgcaaattat
    14041 ccatatcgca gaattagcgc tagaagtaac caaatatgat aaaccaggac tctttgtaac
    14101 agagttgaac taggaaattt ttattcatta tttttcttgc aaaatcttaa aacccaatgt
    14161 ttcgtatgag catacataag aatgattttc aaaatcaaac cgacgaaata tcagactggt
    14221 tttaaaattc tcgcagcagt gttatcataa attagtattc aatctttacg ctctttatta
    14281 ttcctatcta gcggaattaa cttgaaccaa cattagataa ttctaaatca attcttccct
    14341 ttgccaattc aaaggttctt tgttcttttt ctactccgat aaaacgtcga ttctgtctca
    14401 gtgcagttat acctgtggtg ccggtaccca taaatgggtc gaacacaata tcgtctcgaa
    14461 tagttagttt tgaaattacg tgttctgctt ctaccggcga ttgctcccat ttatgtaaag
    14521 ctttagctgg tggctcagat tctatcaagt ctgcgataga atccagcgtt ctcaatctgt
    14581 tgccctttac gaaccatagg agtggtttcc acataacata tacgcggtga tattgcaaaa
    14641 gtctagacct accattatgt tttaccacca tctcccacca atattttaat ccagaatttt
    14701 tcatgtattc aaagatctgc ggtaatgcgt agtttcctgc atacatgacc aggcttccgc
    14761 cttgcttcaa aactctacag gcgagctttc ctaatggttc atacattggc agccactcct
    14821 tgtgataggg cggatctgta aagattaaat caatactatt gtccccaatt gtagcagtat
    14881 caatgtattg aaaatctccg tgaatcatct ttactctaga agcattttct ttgttatgtt
    14941 gagatccact tgcggcggct tcttgcacct ctctgattag atcctctttc tgctcgtgtc
    15001 ttctgatctg attgtaaaca ctggttattc ctacagtatc tcttctgaga ctatttcttt
    15061 ggtcttcggt acctttttcg ataatcttct tgcctctctc atatgtggca gtagacacac
    15121 cgatcttttt cgcaattatc ttcgatacct ttcctttctc ctcacttcgt tcaaggctag
    15181 catcgataga tgctcccctt tgttcgagct cattattatt atcgtcatcc tcttttttgt
    15241 tggctagtcc tactatatgc ccaccaaaag acatcctcct cttcgccctc tcttgttcta
    15301 tatcttctaa tgagtatccc aattcgactc tctgaaagcc attcagctgg cgccttgtaa
    15361 ggttaacctc gataacaaat cccttctctt ctagcgaatc tttgaattcc tttgtgtgga
    15421 attgaagggg aatacgaagc tccttacaag ctcggaatcg atgatgccct tcaagaacaa
    15481 ttccctgttt gttgactatt acaggtatat gcaatccttc ttctttgatt gattgcttta
    15541 acgaattgaa ttctccttca gacaacggtg gaattagctt caggtattct ttgctggttg
    15601 acagacttgt attactgctg ctcttgctga tgttgtta
//
LOCUS       DAOR01000081            3147 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_61307, whole genome shotgun sequence.
ACCESSION   DAOR01000081 DAOR01000000
VERSION     DAOR01000081.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_61307"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          934 a          602 c          607 g         1004 t
ORIGIN      
        1 gtgactgaat tgattatatt tttctgctgg ccgtcggaag caagtgtacc gatgaaaaaa
       61 ggctgcggct tgtggattta ttttgatcgt aagcaaaagt ctacatccgg aaagctaata
      121 ccgatggaag tagccaccca caacattcat acttgcagct actctcaaat aagaaggcat
      181 ggattctttt atacggcgta ggatgaacta ggaccaacat tcctcaatca cacctattca
      241 atgttaacta acaccattaa tgcaacttat tgatggtgaa ctggtcttta acggatccat
      301 cattgctata gaactttgca tttagagtct ttccgttatt tgctacgcct atatctagaa
      361 agccatgtcc tccgttatac tgagtagcta cgtagggagc ctttccaaat agcgggaaaa
      421 gatgcgcacc tgcagtacct actataacat atatccttcc ttggggatta gtgtagttgt
      481 cagtgttgtt atctgttatc agtgggttgg atggattctt gatgttatat ttcataggat
      541 atgatgtttg gtaattgtgt tcatgccctt gcaatacaag gtctacatga tacttatcaa
      601 atagcggatg ataggtgtct ctgaatgtat aaaggggttg gactaatgat ggtgaagagt
      661 atgatgtctg atgataatat acaataatcc aatttatgct tggatctgat gaggtttttg
      721 caagatcact ctttacgaag tcatactgtt cagagcctat tcctattgct ggcaactctg
      781 ttgagagagc aacaaagtga atattttgat aattgaaaga ataatactgc tttgtgagat
      841 tgaaatggtt gatatatgta ttcaaaatgg atggggaatc atgttcgtgg tttcctatag
      901 caattctgat tttttgctcg ataggcatga ctatcttaaa ccagcaatct gggctacgac
      961 tttcactata gtcactatag taatagtcac caactccaag cactaatctt gggttcttgc
     1021 ctgctatatt attgacagta gctgttgtat tggaattgca ggcccaatcc gaaaacggcc
     1081 ccaaagctaa aattgggtaa aactttcgct atccttgcgg catagacata ttctctttca
     1141 ctgaccgtaa ctgcagatag cattatgata aaaaaaatga tgaacggaac atcaagcaaa
     1201 agaaaattag tgtagccgga ttgagttcta tacgtcattt gtcatgctgt ctgttttagt
     1261 tttggtgctt aatactttat gaatctaaat caggtgctgt aacaagcatt caaggattcg
     1321 atagaatatt atggtttatt gtatgggcta actagcagta aagatctggt tcgtggcagg
     1381 agatggagaa tactacaatg tctgaattaa acagaatcct cctctgccta acacatcctc
     1441 tccagagctt cttcttgcga tattattcaa ataatctaat tttatgcgtt tggcgtaaca
     1501 tcaagcggct attgcagcat ccaaatggac tggataaaga caaatgggtt tattgtatca
     1561 atatgaatgg ctatatctca actatcagct aagatatgaa tgatgatgaa attcccgagg
     1621 atataaatgt ccataaacaa atttggggca aagacgcaga tcaagttatt tatggcgaag
     1681 tggaaaaaaa ggaagatgac gaacagcgat gtcccatatg taattcacgt atagatgaat
     1741 tcggttattg tggatgcgga ggaaagttag gagttagtta gctgattaat aggcaacata
     1801 acaaaaaata ttctgatcta agatcgaatg gtatgatgaa ctaattccac tagatcgtgg
     1861 ctgtggaact tgtctataga aaatcgaatt gtccagaatc aaagctcatg ttgttctcat
     1921 ttttcccgaa gcacttacca cttgcttgtc tatcgaaaga gtatcaatat attctaaact
     1981 tttactattc agcaacgttt ttggcaatac aatgtgacag ctttaagata ataaagcgct
     2041 catttattaa tcacacatct agcttttaac tctaggtcat cagattctct ttcatcattt
     2101 tgtagaactc ttcatcagac atactttttt ttccttctat ccatgtttct acatcgtttc
     2161 cagctaaata cctcagagta ggattttcgc ttgtaacagc tttaagcact acctttgcta
     2221 caacgtcggt agaagagcca ccctctatca tatgttgaaa gcttgtagcc acgttttgca
     2281 taagttgtgc atatggagaa tttggatctt gggatttctt ggcagcaact atactgttaa
     2341 caaagtttgt tttaataact ccaggttcaa ccaagaccac tcttattcca aatggctcca
     2401 attcatatgc catagattca ctcaaccctt caattgcaaa ttttgtgcta acataagcgg
     2461 atcctcctgg ataaccaaat cttccagctc ctgaactaat atttacgatg ataccagatt
     2521 tctgttttct catgattgga aggactgctt gagttactct tataacacca aatagattgg
     2581 tttcgtactg agcctttatc tcttccattg caaggtcttc aaacgctccg ttaagtccat
     2641 agccagcatt gtttaccaac acgtcgatct tgccgacgtc agattttatc gaatgtatcg
     2701 catcgtttat tgatctctct tcggtaacgt tcagctgcac aacacgaatc ggcagcccct
     2761 ctttggttgc aactgattgt atagttgcac cttttcctag attcctcatt gtggcgtatg
     2821 taaggaagcc atttcttgcc aatgttaagg atgtttcaaa tcctatcccg cttgagctgc
     2881 ctgtgactac agcaaccttc tgattcgatg aagataatga agagggcatg tcctattctc
     2941 tgtagactgc tattacatta cactacttaa gtaagatagt aatcctctgg ttagttagta
     3001 accaagatct aaccaactta ccaagaaatg actaccatat ttaattatcc cgttacgtac
     3061 aaccactaac aggtgaataa atgaaaaatg gagaatcaat gtagccgtga aaacgatgat
     3121 tcctgcggat gttcgtgtca tcctgga
//
LOCUS       DAOR01000082           11132 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_61306, whole genome shotgun sequence.
ACCESSION   DAOR01000082 DAOR01000000
VERSION     DAOR01000082.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_61306"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    10617..10770
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3535 a         2016 c         2105 g         3322 t
ORIGIN      
        1 taattctata tctacaattg gaattactat gtcttcaatc tttgaggtaa tatgaaatag
       61 atacatggct tcaataaccg acagcaaaag gaagccgaac atcatgaaca atagtctgga
      121 atatccattt cttagatatg caagaatcga aacaaagcct agaaaaatgg ctatgataat
      181 gctaattacg tgtagcaata agtgataaat aattgatgta tcagtgatat gaggaagaat
      241 cacaggcacg gtaactacag caaggactat agctgtgatg accgcaagca attttgcctt
      301 acttgatgtt attttatata aagaagccct gactgtctgc atatctctct tattagcgtg
      361 aattggatat atatccgcgt tggttcaaat atcgaacttt atagaaaaat aaataaattc
      421 tctcttaaga agtctaagca ttatgcaaac ttgtggttta gataaagtgc atgtttagtt
      481 cgacataatg ttctaaggtg tacaatctta ttcggaacct gattactttt tatcatccgg
      541 gaaagcaggg ataatatcag ttagaagtag tgtgcatcaa agatatagac agaaatagag
      601 acctagttgc gatattaatg tcaggtattt cttgttaggt gctttcctcg atgctatgtt
      661 accagatgtc ataatatttc ccactcaacc ttttatccta aaaacacttc ttttatctca
      721 tatctttaat cgatatttgc accaatggcc ataaatacat gattcgacaa cttttagctg
      781 gatatgcaga cggataaggt acctggacga ggtgcagcag caagtagcgc caaattgctt
      841 gccgtcatca cagtatcagt gattgcccta cttgctatcc ctgtgattat tccgcatatc
      901 agccataccg gtctgattta ccacattctc cttcacgttg cgagtgtgat catagctgtt
      961 tttcttaccg ttgtttccat tatagcctat agaaggaatc atagcggtag attacttttt
     1021 atgatgggtg gatttgctgt attagcgttt gttgaggttc tttatttgtt ctattctacg
     1081 gttaatattg aggctgtgat aatcccaata gtagatatag atctctcgca cgttatactt
     1141 ctggtgatgc tgaccttgtt tggtataggg gttttgaagg tgaacagatg atgatgtcag
     1201 taaaacctac aggcagcatt aatgaattta atgatcctaa agagacgctc atgatgcata
     1261 ttaataatat ccctggcata aggtatagag agcttctaag acagacgggg ttgtctaatg
     1321 gcgtcgttga atatcatctt agcgcactgg aaaagtcttc tcaaatcagg gttgatagac
     1381 aaagggaaaa tagaacgact aggtattatt caatcagcat tccaacgcaa gaatctgaca
     1441 ttctacgaca gctaagaaat aatgtatcca gacaaataat taaattcata cttgataatg
     1501 atttatgcac ttttaatgaa atagtagaac atttgggcaa agctgcatca actgtatcat
     1561 ggcatctcaa aagattgaat caagaaggtc taatatccgt caagtatggg ggagaatatc
     1621 agctgtacca aataatgaat cgtgaactgg tgtacgacgt aatttctaaa tataaaaaga
     1681 gcttcgtaga tgtggtagta agtaactata cagagatggt tgaggaactt tagtccatag
     1741 tctatatttt gcagtcctta ttatctccta gttcgagttt gattatccac agagccagtc
     1801 taatcaacat gtgttaagaa tgatgtgtta gagacatagc attgctctta aaaacatccc
     1861 ttgttctaag tatctcaagg ttacttcttt tatttcttaa cgccttcata gtgcatggat
     1921 tattctatga tgataaaacc aatcatcttg cctatgctca tttttttgga tcagtaaagc
     1981 aaatcggtaa ctatcaaata gccaaaccca aggatgttct tgcaattcga tacttgtacc
     2041 ataaactatt ttatgatata ctaacatatc tattcaatgg aaaagctact aaaaagtaat
     2101 tctgcagcaa agagtactgc ctttattgtt gttttcctac tgctaggttc attgtctgta
     2161 gcaataacca ctccattcaa acaagtagtg tatgcacata cttttactgg agatgaaagc
     2221 gcgtcatttc tctcagttat aagaatacta caggccgaag gtaatcttgt acaaacaaac
     2281 cttgcctcaa atctcagttt ggcacaagaa catggaaaag ctgtcatggg catagtaaac
     2341 gcaaatcata catttggtat cttaccaaat gaggtgtctg agaataacaa aagagttgca
     2401 tctgacattg ttagtgcagc aaatgccctc caaaccgcag taaaatcaaa tcccgcaccc
     2461 ataaaagccg atattaaggc gaaaatagat aatcttaatg ccacgttaca ggaagctatt
     2521 gctatacgca tcccaaaaga tcatgttagt aattctacaa ttaacggcgg agctaccaag
     2581 aacctggtga atgaaacact tagacagtac gggtacgcct ttggaatttc aggttctaat
     2641 ggaggtaatg ctagcaatat caataatagt acatctgcta atttaggtgc tttaccgaga
     2701 ggaaatactg ttgtgaacat gtctgcttat caaagcgctc aagccttggc ttcgcaggca
     2761 caacggatgc tcactgaaac aaaagcactc ctcccaacaa acgtaacatc gacaacaacg
     2821 ttaacaataa ctagaattgg taacgacctc tcgcagttga agaacaccat agatacaaaa
     2881 agtccatatg gcaacgtagc aagtttagtc cagaagacta tttatcctga cctcaatgca
     2941 gcatttggtt tgaaataaac gtatagccaa cgactataac caatttcttt tttttatgtg
     3001 agttccttcg caagtaattt gctgcagcta ctatgcaaca cagattacgt tgtccatcaa
     3061 gtgtgtgtcc taagtaaact cgtataattg caaatttagt ctgaaagcgt tgaatatgtc
     3121 atcgtaggaa gtgctatttt tatcaaattt tccgtcgtta tcaactgctg tatgaaggct
     3181 tgtcgtgagt ttttgaaatc ttgttgggcg tctcgctaca agtccatctt ctattaaggt
     3241 ccgagtattg gatagcattg ctttatgaga agtagagaat ggaattggga gtaccatttt
     3301 ccgtcctgac ttcttttctc tgtaagagta tagtctacta ctgatatcat aaaaaattga
     3361 aaatatctct acttcatcag attttgcttc atcattttat agaactcttc atcagacatg
     3421 tttctcttac catctaacca cgtttctaca tcctttccag ctaaatacct cagactagga
     3481 ttttcacttg taacagcctt aagcactact tttgctacaa cgtcggaaga agagccaccc
     3541 tccaacatct gttgaaagct ggtagccacg ttttgcataa gttgtgaata tggagaattt
     3601 ggatcctgag atttcttggc agcaaccata ctgttaacaa agtttgttct aataactcca
     3661 ggttcaacca agaccactct tattccaaat ggctccaatt catatgccat ggattcactc
     3721 agaccttcaa ttgcaaattt tgtgctaaca taggcggatc ctccgggata accaaatctt
     3781 cccgctcctg aactaatatt tacgatgata cttattttgc aacgaatgtc aacgacatag
     3841 aggcgattct gaagtacctg aagctataaa aaatcaacta ggaataaata tagcttatac
     3901 gacactgcat accgaaaata ttttattact aacaaaaatg atagattatg gcttatgaaa
     3961 acgtcttatg aaacgaaagg aaaacaattg ttatggccgt tattcggatt agttgctttc
     4021 gagattctcc tttcggctac cgttagtggc tatgcaatat cctcgaaggc tcttgacgtg
     4081 agtataacat cgcctgagaa cggtcagcag gttccagtta gcaaaaatct cttaatctct
     4141 ggaatatcct cagctaatgg gactaataat tgcaaggtat ctgtaataat aaataacatc
     4201 aagccatatc agaaagccgt aggtataggc ataccaagtc atttgaccgg ccacatttca
     4261 aatgattatt ctacgtgggt ttaccgtctt ggttttccgt atcctccgct taaactaggg
     4321 gaaaacaaaa taactgcgaa aataacatgt gcagccaaca atcatgcaat atttaacacc
     4381 gtaaacgtaa ctggagtgca gtaacaagga caaataccac tatcgatcca tgatgtcaat
     4441 gggctaatgg ggctaatggt tcgtctatca aaggaggtta agagtgctga gaagaatcat
     4501 tacatcaaaa gaaagatgta tgagagtaat aacttaatta aaaatgcgga agctgtcgtc
     4561 gcactactag tcacacaaat atacctaacg cctcgaaaag cacaattatt gtaacgctgt
     4621 tcataaaaag gagtatcgca ggattccttc ttgcgaccgt tgctttgttt tgaaggctct
     4681 gatcagtacc aatgattgca tgataaaggg aatgctattg cagcaaataa aaaattggat
     4741 gtttaggctt ttggtagtta ctgtagtgct attgttcttt ttccaagatc tacattacat
     4801 cgatggacca gcattgttat tcagaccacg atggccagag aaaaagccgt gactatgggc
     4861 atacatcccg ctgccgcaat aacgaccaaa cattcctctt ccacctggtt gtatcaatgc
     4921 atttgcgaat gggatttgta tagttgaaag tacttttccg tttcctgcat gtactatcac
     4981 tctatggata ttgttgttag catccacaac atgggccgtg taaactaaga acccattcac
     5041 tactccaaga tgagcagaaa ttacatggga atttggacct acaacttttt cggcgcttgt
     5101 agcagctgca ctcaaactga cgttagcttt agattgaatt atttgcgata gttttggaaa
     5161 tacctgtact gatcctgtca agtttttcaa aggaacagca cttgtattac tagtactgtt
     5221 ttggttttgg gcacttgctg cttgctgcat atatatggaa accaaaattc cagtcgttat
     5281 cgatgtagtt acagctaaca tcgcaatcat taacaagttt ttgtttttag ttatcatttc
     5341 tttttttgtt tcacctctta gaaagaataa cgcaagagga ttaaattaaa acatgctaaa
     5401 ccatgggagt taacaatgtt aacttacaac gttaatcaag tccttaatat tcatccattg
     5461 aatagtatga cagtgataac aaaggtgaca aaagtcgagt tcagataata acttctgcct
     5521 aagaatgacg atagctagga agggtggagc tgatgctagt atcagtggat ctatgccaag
     5581 acagcacttg atagatgatc ttctttaaat tattattcaa caggctgctg ctcgaagcac
     5641 aatgcacacc gtgatgtctc gacgtggttc aaagatgaag aaggcgaacg attatgataa
     5701 tgggtagcat atttagatgg gggaaacaaa aaagagcaat gcatagtcta aggtgtttcg
     5761 gattcgtgct tgtattccta aatgaccact gtgagatttg tgatgagtat tgtccttaga
     5821 ggtagcaaag atcaaaggga tacctattaa gttacatttt acattagtcg ttatctcttt
     5881 cctggttgca tggacactag cagtaatgct gatgcccgaa ctctatcctg gcttggatat
     5941 ttctgtatat tggattatgg gtatcatagg tgcaatcatt ctttttgtat cagtacttct
     6001 gcatgaacta gtacactcca tggttgcact aaggtatgga cttaaggtac gtgagatagt
     6061 gctctttatt tttggaggag tatctgtgat tgaagatcaa gatgaagtgg cgtccaaaga
     6121 ttttcgtaaa gagttcaaaa ttgcaattgt aggtcccata acaagttttg ttattgcggc
     6181 tatattagca acatcgttat ggctcctgat gtatatgacc gtaggatctc ataataatgc
     6241 tggcaatgta tcagcgatta tagcagcggg tgtactccaa tacggcgtac ttgttaactt
     6301 acttcttggt ggatttaacc ttatacctgc gtttccttta gatggcggga ggatactcag
     6361 atcagctctt ctcagatcga aaaatgatta cgataaatct acaaaaatca cagcaataat
     6421 tggaatagcg atatcttatg gattcatggg gttgggcttc ttcaccatga tttctggttc
     6481 atatattagt gggatatgat tattgcttat agggtggttc ttaactagta caaccaggtc
     6541 ctatttagca gaacatcaac tgagatctat tttatcgggt attcgtttag gcagtataat
     6601 gaatagccat attatagccg taagaaagga tatgctaatc gatcgtctaa tacacgaata
     6661 cttcaataca cccacgaagg acttatttcc agtaacagat gactttggtc atctagttgg
     6721 catggttact gccaaagacg ccagcagagt gccggaatac agaagaaatt tggaacacgt
     6781 aatagatatt atgatccaaa agtcggatct cataatcatg aatacagaga gcactgtaga
     6841 tgatgtacta aaacacatgg gtagaaagcg ctctgagaga gtagcgttta tttgtgatca
     6901 agaacgaaag ctaatgggtc tgattagtaa gagggatatc atgaacgcag caattgaaag
     6961 aaaacaccat gtagaggcag tgcacgtcac cagtcgtggg aattaagtat taactaattg
     7021 ttaaattgta tacagctgta tgtgttgacc ttgatgatat agaagcaaat tgcgcactcg
     7081 agggttttgc cggcactctg actattatat cctcgttgtt gtagactttt tcctttttag
     7141 ctgcgataac gttgttgtta tgacgcagga gaatgtcttt gtagatgaga atttttttgt
     7201 tcttcacata caatatcttt atatatacta tagctgggat agagtgtttt tacttctcat
     7261 gtccttaacg catccacgtc tctagatgta cggattttac ccacattttt tgtatgccgg
     7321 cttctcagtc atcatatatt gtccacttta attctctgat ttttgtgtct atgtcgaaaa
     7381 ggagaaaaga gacaatatgt caaagatgag tatcgcacta gacgacgagt tgttaaaatg
     7441 gatagataag caaatacaga ccaaaagatt tgctagtagg acgcacgcaa ttgaattcgc
     7501 tgtacagcag cttaggagaa cataaaacct tctgcttagt aaataaacta tcgtttctat
     7561 ttgatacagt taattcgatt accattttct tgcgaggaat gattgtaagg ggtgggtcaa
     7621 ctcttgtagt agtaatactt tccatgacct attattcttg tattatgatg ttaaggttac
     7681 tcagttcaag tgagtccaac ctttgttatc ttatccagta tctcgactgc cattacagaa
     7741 tagtccaatg cttctacttc aattccattt gacgcaaaac tctatggagt aaacctccaa
     7801 ggagcaaatc tcgatatagc gcacctcaag ggaactcata atttaccaat ttcaaagaag
     7861 gaggcaaaat caataggagc gttcgcataa gagccacaag caccgtagca cagggttctg
     7921 ctgaaaattc actggaattt actttcatat ttagttctca attagctttc gacggatatg
     7981 atgctaacta aacagtcata taattcctag aagtactggt ggaagacatg atgtttcaaa
     8041 tttacaattc ttgtgagttg aacaccatag gaataaagat aaccagatat gacagacgat
     8101 cctattagaa tcagggcata tgttgcattt aaatgagcag cagttaggca atattagaaa
     8161 ttatcagact aggagattga agaaagacag gaagagtaaa cggatatgaa gaataatgaa
     8221 gagcatcgta aaaacgttga cgtgtatttt ggcctaagag gcgcagtgac gactttcaag
     8281 gaaatgctaa acgctaagga cgcagagttc ctttctcgtg gcgcatggcc tcagatctcc
     8341 attaatacac atgtcgaaga aatcgagagc atattgaaaa aggcgtacga gaaatacgag
     8401 ataaggtttg agaatgttgc tgaggtagaa aaaaatgaat aactaggcta gagccacaga
     8461 tggcggtcaa ttggaaaaac ttgtgaagac aatatgactt gctggagtat ttttcctatt
     8521 gctactgccc tgctgagact ggattgcact tattggaact gtgtaaccat cttttctcct
     8581 cctgcgcttg acatgcctct aatacgataa cttgttacta tatatgataa caggggtaac
     8641 aatattgaaa tctaactttc aaatcctttg catctttcat ttttgagaat ttccttgatt
     8701 tcacaaggca gcttctaacg gcgttaccta atatttctaa tccccaaacg aatcatgaca
     8761 tagctggcaa acaataaaaa tttgatagat tttgagacat cgatagggat ttacaacaag
     8821 gagggccatt atgggttcta aaactggaaa cgtatactgt caaaaggggt tatggaatat
     8881 gtacgtcaga tagcaggaaa gtatcacatc ctactacgag aaagatataa caggaaaata
     8941 atattaaatc atcacatatt tcatcactac aacagcttgt cagacttaat cttcgaaaat
     9001 tcaaacacat gcgtaaattg tacagtacaa tcaatatcat gacatcgcta ggattatttt
     9061 tgataggtat acttggatta tcttgttagc tgttactatt tccaaagaac ctagaagttt
     9121 caatcagatg atgagataag agtcacatta ttaaccagta attttcatct ttcttcgttc
     9181 gggtgaaata tgaaatctat aataccaaaa tagagcacct gccataatta tgaaatccgt
     9241 cacataaaat atatcccaaa ataatagatt gtgcagacgg ccatttacaa agtcatttac
     9301 atatcgctca tccgctacag cattgactaa cagagagagg gatgaaagaa tccatggaat
     9361 ggattgtaca tcgtcctttc gtaagctaat tagtattatc attgagggaa caaataacat
     9421 catatctaaa attggatagg tgatagtaat aacaaaagct gtaaaatcaa tttcggcaag
     9481 aaatcgtgat gaagggaata ggttaatcgc aatgtaagta atgaacaaca gtgtaactat
     9541 cgatgttaga atgatagtca ttaatttgat cctactgcgt atgaatcgaa ccactgtgaa
     9601 caaatgtgct gcaaggaata gatatccgat aaaccataaa acatccgtaa cagatacaag
     9661 tatttgttcc tctataccta aagcaaaata gtaataagca agagtcaggt ctgcagcgaa
     9721 ccaagaaata atacctagtg ttagaaacaa atatgatcta ccatgaacgt catggagtcc
     9781 atgacagtac actacaatta tacctaaact tgaagcgata gctgctgtta tatttagaat
     9841 caaaaaagta gaagaagctt tattgtgcga atctgagaaa attattatgg aattcgcaac
     9901 aataatcgca acaataattg cagaaatgaa atatcctagt gttttccgtt catgtattgg
     9961 cttttctgtt gacagatcaa atcattttca gataatcttt ttaatgtata aacattttcc
    10021 ttacgaatct tattcctgct gatttcgttt tagatcattc ggctcgttta ttctatttgc
    10081 actaacacca agtgattcca attcatttag gcaccaaccg tatctttctg gatgttcttt
    10141 ttttgtggac ataacagtaa gacatctcat cggtatatgt aaagtaggat tctattgcaa
    10201 cgcactgcat tagtatttcg tagccattga ttactaattt gcataagttt taagggtgta
    10261 gacggcctag atagagagat tgggaatagg agaattatga accacataac tctagcaaac
    10321 tattctctct cgtctcggcc caatctacac tctaaatcat tagtattaga tacgagaata
    10381 aataggattg ctaaatctat acgctagtta ttatcgataa aagatatctg tcatgccaag
    10441 agtagtattc tctttattag atcaagtgac attctgacta taatctttcc aaagaattta
    10501 tcagagtttg tatatatcga caatttctcc tacagtttta gcacttgctg tgaagcaccg
    10561 caaatataga atagttgacg atagccctat gggaaatgaa ccacaacgta actttannnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aatgaaccac aacgtaactt taggcatcgt
    10801 tgcaatcgtc gcagctacgg cgctgacagg agtagcattc gccgtaccac aacaagcatt
    10861 agcaggtgga caccactaca aacacaacaa tcacaacagc aatagcgtaa aagttgatca
    10921 acagatcaat caactaaacc agtgcacagg cgtaccactc gaaactctta ggacattaga
    10981 taatactggc agtagtacgg tatgccttaa ccaggccgac aactcagctg acatacaccg
    11041 ctgatctaac acaaatcgag tagatatatt ctctccatta tttttttcga gatatacgag
    11101 ctcgctgtct ttcagtaaga caatgactgg tc
//
LOCUS       DAOR01000083           24471 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_1611, whole genome shotgun sequence.
ACCESSION   DAOR01000083 DAOR01000000
VERSION     DAOR01000083.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 24471)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24471)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24471
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_1611"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    10850..11063
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23874..24003
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7462 a         4497 c         4613 g         7555 t
ORIGIN      
        1 cttctgacgt ttgcctactt cagggattgg gaactcttgg accgatatca aagtcttttc
       61 catacatggt cttgatatat ctattactgc tagcgtcatt tattcttgct cccgtagata
      121 ttttcttaaa agtccttcaa acgtctagtt ccacttgttt taattaattc cctatctcgc
      181 ttacacatac aagctgagca aagttgtcca accgactgaa caaagaaatt aacgatgtca
      241 ggtttattcg cttctcgttg tttctttttt tccttattga ttcttgcatt ctctaacttg
      301 gctgcctttt cttcggtcat ttgcttaggc aaaattttgc ctgagttact attctttcct
      361 cccttatcag aaggtttacc tggctcttcg ccccccactg aatctgtctt aaccttgcct
      421 cctcatcctc tatcttctca agttcaagag caagatcggc catttggaat acttctaagt
      481 atctgcgttt ttcctagcgg agaataacca acaaagccgt tagaaactat atgatatggt
      541 catagataat atcggtagat ctggcctgac tccatttagg attacggctg cgttaggata
      601 actcaacgtt cccgccatcg tggttgataa ggtcatacaa ataagcccga accaaaacag
      661 gctcttgcgc aaaatagtct tacaaattcg acctagttcg taagacttta aaaataaaag
      721 actgacaaaa aatcaacctt tgtgtgcaga aaattttgcc agtgttcatc agagaaggca
      781 gcacaactag aaaaggctag aattaataga gaaaagttaa gacaaataca actggttata
      841 cgacacctgg tagaaagaaa tcggaatact cctcgtctca tgaagtgctc attaatcgca
      901 accaattcat cgtcttatgc aatctcaaaa aatcatatac cttaacaagt catatgattt
      961 tttcttctct tatgcaattt acacctccca tggtatatgt atattgtatc agaaaatatt
     1021 ttaatattct agctttttca agagttttac aaatttatat tctttttctc atggtttttt
     1081 ctaacctcct atcaatggat taatatgacg tactcacttt tgtttaggac atttaatgca
     1141 agaattagat ttcttccatg aaatatgctt gactgcatta ttttctagtt tagctgtaaa
     1201 gtttgcggca gttcttgaca gtaatggaaa gctgattgta ggtgaatata gaagaggcat
     1261 tcaaagctca tcccgagcta atttcacacc tgaccgtaat tattaccatg caagttaccc
     1321 tttctatctg gattacttgg ttcccgctac agtgaaaaga agatttcgtt atttgggcta
     1381 cgcagaagaa gaacttcatt ttgaaataac tgaaataggt gataatgtaa ggttggctgt
     1441 cagtccatta acagaaaaca aggataagta cctttgtatc tgtctagaat catcatctcg
     1501 ttatgaagac gtaatttcaa aattaaacaa tgccatcgtc taatatacta acctgaatag
     1561 accaactaca aatcgtcaga ccgttggaga aatgcctagt ttcctcaggt aacgcttgca
     1621 atcttaggct gtgctggtct aatctctatg tttcgtcttg ttgtccccat accctctcag
     1681 ctattaagga ttttaagata tcttacacgt ctaatcttca aagaaaggtc taaactgaac
     1741 atcattaccc ggcgagttat ttgcaaattg ctttatcgaa tcattttaca tattttgtct
     1801 tgaaactctg aatgttgatt aatttctttt acaaatacaa cacacgaatt cacgactcac
     1861 gatatgtcat ctcatgccat ggaaattctt tgtattggtc ggtcaggccg atatagggtt
     1921 tttttatctt tttatcaaac atattattta ttgcacatat ctctacatta cagtagattt
     1981 gttaaggata gtgcttctcg gtattgaaat gtttctgaag ctctttgatg gaattctaga
     2041 ttagcttgcc ttgtcacctt aacagccagg aaaactggac ttccaaattc tttagctctt
     2101 catgatatgg gactttctac tattatagga aaaccgaatc aagatgctag tgatcatact
     2161 caacccagct atgcgttcaa ggatggaaag gctaagagga tgggacctta gagtagcagc
     2221 gtctcctgta gtacaaatgc tccataattt tttttgagtt ctccatcttg ccaaccctgg
     2281 tcgtagtcag agattttcgt tttgttcttg gttggttgag tcgggttgtt ctttcttttc
     2341 gtctttcttt ttcgtctttc tgtattcttt atatgcttca ttcccatatg tagatggcga
     2401 ctctgtattt tcttctgatg ccttgatatt gctcattagt cagtcttagt cctgtattga
     2461 atatattagt atcaggatac catggtatct cttcatagcg tcctgtttct attaaccaat
     2521 tgataatttg atgctttagc gcttgaattt ttgatgggat atttggattt atagcctgtc
     2581 tcaattttga agatttgtgt ttgtgtgtgc tcctttgcga agccattatt aatgtgattt
     2641 tatcattatg aacgaattaa ttgagagtgg tgcatgatct taggcccaga atttaacgta
     2701 ttataggcct tgaacaaagt ttctcattgt catcattcca atatgcattg tagtaaggac
     2761 cgtgcgtcta acatttttca tagaattctc aatggggtta taggaatgaa atattcgaca
     2821 tctgaattac cctgtcacct catatgcgac gtcaatatct ggataagctc gtatagaatc
     2881 cttcggaaca aaccttaata acttgaattc actttgaatc agatctttaa catatggaga
     2941 tatgataatt tgatcattct ccgcgacaga ttcaagtctg cttgcgagat tgagaggaga
     3001 tccaaaaatt gttaccgtcc aacagaacgc taatttgatg ttgtttttga tcacctttat
     3061 ctccgtcttg caattagaag accatctgct tttcagtata tatgtatgat gccaaacatc
     3121 tcgttgaagt atgatagagt atattacaca ttacaggaat taatgcatga atactgaatt
     3181 tgagaattta tagcatttta gtcaaagata tatttaagac actatcatcg gtgaagtgaa
     3241 ttatgagata aagcttgcac gttacattat tcttttcatg tattatgaat agtttgaata
     3301 atttattccc ttaaccagtg ttgacttatt gctgctacta atgtttatgt tattgctatt
     3361 atagaagaga cgaggattaa aaccaaaccc ctaacattgt tgtcatcttt attatattgg
     3421 tcatcatgct ctaacaaacc ccaagttgtt aaggtatgct gctagtttta acaatgctct
     3481 ctccttcgct aggaattgta gggttgatca taaagttatc cgtcaaccct tcgcacgaaa
     3541 atggcaaagg ccacctatga catttattac acttgctggt tgttaaatga ggatactttg
     3601 agttattata gtaattaatc agaaaactaa attcattcac tgtctgctac acaatatgac
     3661 attgaagaag ccctttatga ctacgaaagt cagaagtcag aactcacgcg aatcctcgca
     3721 aggagaatat cagctcggga ctatacattt aaggattgca aaccatttgt tttaatacat
     3781 ggagcaaacg ttttgacaat atcacaaaca aattattttg gagtctgaac attctacaac
     3841 tcctccattg gaatttagct cgatgaatct gtttctgatg tgtcgacgat cagtcaatca
     3901 acaaaaaatt aaacgatctt ctcttttgat actattttct taattccttt aacaatttta
     3961 attatgctct ctggtatgtc tttggaagct ccggtccaac ctgttgtgtg aatgttatca
     4021 cccattgtaa tggttagagt atgtgcgact agattctcat cttctttttc cggcgggtaa
     4081 tcagttttag tattaaagaa ttcctttgct ctaactactt cttttagttc ttgttcatct
     4141 gattcagagg gctgtttctg cgttgtatta ctaccactaa catctgtaga ggatgtgata
     4201 atattcggct ttgaattata tgaaataatc agataacggt ttgatatacc gcctaattct
     4261 gtatatttaa agctgaattc tgtatttgat gaatctctgt cgctattttt ttcatttctt
     4321 ccctgaggcg gttgttgttc gtccatttca atgtctatat tgctaacata gattaagaat
     4381 ctattgtttg gtcatttaat ttaatcaatt taatttaact catgtttttc agattcttaa
     4441 actagttctt tctcctgtta cttgaaggtc agaagggcta ataggaacaa gaattagctg
     4501 ccagccgtat caaagttaag gaatatcagt aagtgaacat aattcttttg tttcacctag
     4561 tattttctca gtctataaag aacatacaca aatgcaaatg gcaacacggc aataaaatat
     4621 ggcaattcag ctatcaatgg tgagaacgca agtaccattc caaggtataa cgtgaagccg
     4681 gtaggaatta gtccaaatat tatgaatcta tgagctcttc ttttgaattt atccacgtca
     4741 gtataaggaa attgtaagtg atgataaatt acaggcataa caaaaaggac actgataggt
     4801 atggaaaata acgctaatac aaggataatt ttttcagcat atatcagctg atttacacct
     4861 ctaatagata tattcaatag aaaaccaaat aaaacagcgc cgaaggtagt taatcttgca
     4921 ctttctttta atacggtatc gtaatctcca aagagtctgt ctttcttgac gtcatcatcg
     4981 tgcgagcgca aatctagctt ggtattatct tacagtgcga gccgttagta atgattgctc
     5041 ttaatgcatc atatacttca agttgcatcc tgtcagattc atcagagact ggggtttcta
     5101 gcaaatcttt cttcctggtg ggaagacgaa aagaatcgaa ctttacggtt cttagaagaa
     5161 gataacccag ctaacctcat ttcttctcga ccactatctt tggaatatca tccagttcaa
     5221 cctcgccagt atccacattt acagccgtgt ggtgaacaaa tgctataatc atttgttgca
     5281 tatgatcttt ttgaattttt tcatatttga tatcatgtat tactactcct ttttgtccag
     5341 caaacatatt tattttgtct tctagttttt gtaagtcttc ttttacgtta ttagttgcgg
     5401 attggtttac gtattcatga aatattttta ctatcatttc tcaacactca tggaatttag
     5461 tgagacaagt ccaattatac ttttggaatg gacgatcagg ttaaaagtct tcacaaatat
     5521 gtcacggggg gaacaacata aagtatttgg cgaaatacag gcagacgttg atcgttacaa
     5581 tgacatgagt aatatcaatt gcagactgga tcattgtgtg ttgccacact aaccagtttg
     5641 caagcgtcta tgtcgttgcc gttgttagta tccaacattc ctgtagcaga agcaagtggg
     5701 ggaatgaagg acacgggaat atcgtaatcc taggaaataa atcattctgt catatcgata
     5761 gtaatttctc acagtaggaa aacctggtat aataggtaat tgtaaatgag tataatcaag
     5821 tcaataatgg aaattctcat acactcattc tcttgacata tcttcataca ttttcgaact
     5881 aagattacca ggagtatatt cagatttttt taaatacagt gattgaccag gaagtctagt
     5941 tccgttcttg tctgcttcta gatatagatc ctttaatttt cttcgctcgt caggagtggc
     6001 ttcgtaaagt tgcattagca tttcatgacc tccattttgg acttgatttt aggaccaatg
     6061 aaatcaagat ttgtatcata tcacgtatgt agctagtacc actcgttcac tatggtactg
     6121 ctgagtcggt tagcgcaata gtgctataga tgcggacgtc tttactcgtt ctatggtagt
     6181 attttggatg tgagagcaag tccttagcgg tggtgatgga tacattatac ttgcattttt
     6241 tatggttgag tttcaggata catctaaagc aatagaacaa tttgcagaaa gaagtcctgt
     6301 tgccgtacgg ggaatgggta cagaggtttg acccaaaaca ggtctgccca tgtaaaagga
     6361 tagctgtcta cttgattgat gaaacgcaga tgaaaatagc tttggcattg catagatttt
     6421 atatttgaag atgatggcat cgcagcgtct actaaatcga ggctgttact ggatttgtca
     6481 aggtggccta tctgcaacgt tctatcatca tctttactgt tgtagttggt taggggtagc
     6541 gacggattaa ttgagacctt ttatgactgt agccactgac aagttccttt agccattaaa
     6601 agttattagt aacgctcaat taccggctgg ttatatacac aagctcctgg gatatctggt
     6661 tcgatatttt gaaagtagat tgtatatcca atatcaaatc cttattgtag atcaaaaagt
     6721 ttcattagtg atggaaataa gagatgattc aaaacggaca ttcgatgagg caattgggtt
     6781 atctacctac tctactagta aagccggggt tttatcatat gtttcaattt ttgaaaatct
     6841 gtggctacaa actgaattat atcagcaaat taaggaatcg agtaccagac tacagctagc
     6901 taatgaacaa cttgcaatag ctaatgaaca gcttaaagta catgacaaaa tgcagcaaga
     6961 gttcatcaat gtagcagctc atgaattacg aacacccatc cagccgatac ttagcttaac
     7021 agagcttctt agatctaaaa ttatggatag taatcagcgt gaattactag atgttattat
     7081 ccggaacgca aaaagactac agaggctttc agataatata ctagatgtta cgagaattga
     7141 aagcggcaaa ctgaagttaa gtaaagaacg atttgattta aatgagaaaa tacataatat
     7201 catcagagat gttaaaactc aaatttcagt agcaaacgat gtacaaataa cattatcacc
     7261 atctgataaa tcagaaatgt ctattttcgt agaggcagat aaggttagaa tatttcaggt
     7321 tatttctaat ctattacgca acgcgattaa atttacagat aaagaaggaa ccatttctat
     7381 ttctttaaaa aaaagagaag gaacagaggg tgaaaacaag aaggaagggg aggtaattgt
     7441 tagtattaga gactcaggtg ctggtatacc ttcagagata agacataggc tattctcaaa
     7501 gtttgcaagc aagtctgatt ccgggacagg cttgggattg tttatttcta aaagtattgt
     7561 tgaggctcat ggtgggaaga tatgggttga gaataatgct aacggcagag gaactacctt
     7621 ttactttacc ctgctattag ctaactcgta actaccccac tcctaactat catatatata
     7681 atgttgtgac aatcagtaac gataccacaa aaggtaaaaa cggtgataat aaaaaacctt
     7741 tgttagtaga cgatgagtga gattttgcca ttactctaaa aacggtctta ggggccaatg
     7801 gttttaaagt agattcattt aacgatcatt ttaatgtcaa ggattaagag gtcatacaaa
     7861 caggcattga aattcttcaa tacaaaaaaa ggatccaaat gaatggcttg gatccatata
     7921 gagaaataaa gaaggtagat gctagagtta aggcctgctt cctgacagct ttgagcgatt
     7981 tgcaagaata tgaagactta aaaaaaaagt atttcaagaa ggtgaaggat attctattgt
     8041 aaagccgatt gataatgagg agctgttaaa acgagtaaat gaaatgataa cagtaaatga
     8101 cccactagta gccagtagta atacacggcc attgtgttaa aattcgaaaa taataataat
     8161 atgtacagtg agattatatc atatgaaaat ttcggtatgc atggttcgat actacaagaa
     8221 gtttgtgata aagtagtaga acttgacaac tctatcaggt ttgctggttt tgccaacaac
     8281 gcgggaaaga taatcgcaca taaatataga aagggactgg atccgttatt gagatccaac
     8341 gaattagaac tatcttttat agactctgca ttgaggatga ggactagaaa agatatggaa
     8401 cccaaacttg ggagagcaat atactctatt acattatatg aaaaagtaaa acgagcaaca
     8461 atatcgttcg ataatgaaga ttatcctatt ctcatgatct cctttgataa tagcaacaaa
     8521 agaagtgatc atgagtcact aattttggat agaatattgc cactagtttc tcatcacttt
     8581 tccaggacct aaaatgttct gactgtaaca gataaggctc tgactggctg atgtcatcgt
     8641 agcatatagt gaggtattgc ttgtcttaca ttctcggcaa tttgttggta gtttcactct
     8701 agtttgactg gcaatccttc ctgtatcata gatgctattc gcatagacaa ccattagaat
     8761 gtctaaatct ggatgtctag tttttcttcc agttaaggct gaatcagcga attagccggc
     8821 tataggaaac aaccggatgt ctgtcgatcc ttatatgcgt ggccgcgtga cagggcgtaa
     8881 aagctatgtc cctccattcg aattggggaa acctcttgag ggggcatatc tgtaatatag
     8941 tagagtcaaa aaacaatcag tttgtcgtcg gtgattatgt cttaagaatg aatgggtgga
     9001 gggaatatta gatatcagat ggtaggtttt gcaaatacca aatcggagtg aaaaacaagt
     9061 tacataatag taagtaagga tctaatacat agttatatag acgttttaac gactatatca
     9121 tgatacaaac tgttagtgca aaaacacaaa acaaaggaaa ttaacaataa ccatagctgg
     9181 aacgatcctt tgttcgaaag aaagatagaa aacacgactg agggcctgaa ccacatcgtt
     9241 ataccttcct cattccaaag aaaaatcgct cttggcaaaa ggagtagtaa gagatttcta
     9301 aattatttta gaaattgctt aaataagtcc ttgtttttct attggctgga ggaactgtaa
     9361 ggctcaaatg tcctgttatt tttctatctt tctagttgct ggtttaacta gactcagatc
     9421 ttaaaggatg gatcaaacaa gtttggataa aaagatctct ccacccattg tcagagcaat
     9481 agcggctgga attattctcg ttcttctagt atttgcaaat tcaatgatga gctatagcta
     9541 tgctgcaaag aacactccat tccggaacca gagtattctt cctgggcctg ccccattgcc
     9601 aatccagcct cctgaaattc caaccccatt gccaaccccg cctcctggat tattaggaac
     9661 cctcaaagta accacgaaag tgtcaggtgg taacaagaac ccgtcagact ttacgataac
     9721 tgtctcaggc aacagtccaa aaccggcatc gtttgtcggt tcatcttcag gtagtactgt
     9781 cacactaaaa cctggaacct ataacatctc agcctcatct gtatcaggat acactactac
     9841 atactcttca ggatgttctg ggagcattgg tggaggtcag acaatcaaat gtactgttac
     9901 caacaaatac acttctctac ctggatccac aacgttcttg agtgttatta tcaatgttga
     9961 caatactaat ggaggtgcca agaaaccttc ggactttacg attactgtct ctgggaatag
    10021 tccatcacca agcacatttt ctgggtcatc ttcaggtacc tccgtcacat tacaagccgg
    10081 aaagtatacc atctcagagt catcaatttc agaatacact actacatact cttcaggatg
    10141 ttctggtact gctagtggcg gagtaccaat caactgtact attactaatc tatacacccc
    10201 cgtcgtagct acattggtag ttactacgaa agttgacaat actaatggag gtaccaagaa
    10261 accttcagac tttaccattt ctgtgacagg taatagccca aaaccaagtt cattttcagg
    10321 ttcatcatta ggtagcacta tcacattaaa acctggaacc tatagcatta cagagtcatc
    10381 cgtcttaggc tatactgcta catactcctc aggatgttct ggcagcatta gtgcaggtca
    10441 aaccgtcagt tgcactgtag caaatgaata tcaaacaagt ccacttggta agataacagt
    10501 tgtcaaaagt gtaattaaca atgatcgagg cacaaagaag ccatcagact ttatgatatt
    10561 cgtgaccgga aatagcccgt ctccaaacaa tttccctggt tctgcgtcag gcacgtcagt
    10621 atccataaaa gccgggaatt atgaagttgt cgaggcgtcg gtgtctgggt acacgacgac
    10681 atattcttct ggctgttctg ggagtatcac cggaggcgaa acgatcaaat gtgtcgtcag
    10741 taacgatgac attcctccta tcccttttat tgacacggaa acagtgaagc tcggaaaatc
    10801 aacattccta tcggatgcat taaggccact gattgacgtt ggtccttcan nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnngccggta tcaatccttt taccgggact caagatagag
    11101 taactcattt cacggatcta ctcctttgga acaatggacc ttctgctata caattccttg
    11161 gaaataacac ggttacaatg aaagtaatat ttgagcagcc gcagttcgtc acaccaagca
    11221 tcacatttgc cagccataca acgacattgg cagccgacct ggggatactg aaccttgaca
    11281 gcagctctgg cggaggctcc ttactttcga gtctgggttt gataccactg attgacgttg
    11341 gtccttcatc gaacatcatt ggtggacacg tctcacttag ttcacctaca ggcagcagcg
    11401 atcatctgaa gctgatagct gcagaaatta cagatgcagg agtacagcat gcagtcgcta
    11461 taaacttgac aaagacgttg gacataacac caacagtaca atcgctatat cataccgatc
    11521 taagatcaac ctttgccggt atcaatcctt ttaccggggc tcaagataga gtaactcatt
    11581 tcacggatct actcctttgg aacaatggaa cttcaagcat cgtcttcagc ggcgacgact
    11641 acctcacaac tgaagtaatc tattttgcga gataaggcac taagatatat ggaaacttaa
    11701 aacatatcga aaaaccgaaa actgaccttc tggttataat gttgccatag aatcggaaag
    11761 tgtgatagtt gtggtaagcc tcttcagatg ccatattatc tatactctca taatcttaaa
    11821 accggcgagt atgtttctgt actggtggta tttaacccca gttaattata ttatgacata
    11881 taaatagtag tctttctatc tatctgttac atttgtcaca ctatatctta cttataaatt
    11941 ggacggagca agggatcaac aagatcaaag aatcttcaga ccgttacagt tccttcaaag
    12001 catcggtcga aaaagcaggt ggaaaattga ttggaggtta ctatactttc ggtgaacatg
    12061 atgtggtaat aattatagaa gctccaaatg atgaagcagt aatgtctctg atgctcaaag
    12121 ttgggtcata tggaaatgtg agaaccaaaa ccttgaaagc atttaccgct gaggaaggaa
    12181 tgaagattat caaggatctt ccataacctt ttgagtaaca gacagatgac agatggcaga
    12241 gaaagtttca cgtttagaat tgacgttcaa agactcggtt gaaacatttc tcggagatct
    12301 accactcgat gtgaaggttt ggaacggctt gctaatcaat aaaggaactg ttagcatacc
    12361 tacacatgaa aagaataaat tgtatcaagt atttcacgtt ttatgcgtgt tctgatcatt
    12421 ctcagaggcc cacctgggtc aggaatgagt gaagtggcaa aggccctgaa agagagtgct
    12481 ggaaatagca attacgaact attagatttg gatcagacaa tagaagataa gtttgaaagc
    12541 aacatgatag ttgcattaga taaagaattc gtagtcggag aaatgcactt tggcgacggg
    12601 catacgacag aacctgaaat gaggataaga agattcaggg aaaaagatta caaaatgtta
    12661 tcagttgttc taagggtatg cttcgataca tgcgttaaac gagctatatg cacggaaaag
    12721 catccactta tcccaactga cgctataaac cagtttaatc tcttttatca aaacaaattt
    12781 ttgtacacaa aactaaagtc gaagaaattt gggtaaacaa cgagaataaa aacccatcag
    12841 aagtagctaa ggaaataata ataaattcta ccagtgtttg ttcttgagcg accaggttag
    12901 aatagtcttg aaaatctata gtcaatagta gctccagtag catttaatca atacttcatg
    12961 ctgatcgatg caactccctg ttcctataac agtgccggta gcatttgaag atgcagcgta
    13021 ggcctggtat cattcgtacg acatatctat aatggtgatg ccatgcatcg cgaacggcat
    13081 cttcagtatc cacgtgactg gcgacgtaaa accgcttttc ggcggtctcg attggccgac
    13141 gttccatctc gatgacctgt caatcgattc aaagggaaac gttcatctgg acggcggttg
    13201 gctggacctt aaagatcagt acagcttgga tttccatggc ttcaaaattg aagttacaaa
    13261 gctaggattt ggtaagacag aggaaggtgg taaatggata ggcatctcgg gtggattaaa
    13321 attagttgac ggtttatcgg cgggtgcatc agttgaggga ttgcgaataa tctggtacga
    13381 tgacggtagc ggtagagcta caaggatcac tctgaacggt gttggcgtcg agttcgaagt
    13441 gccagatgtt ctccgtttca agggtgctgt atcctataaa gaactcacag tcggcgcaga
    13501 tacaattcac cgctttgatg gtgacattaa gctggaactc atttcgttgg atctcgaaat
    13561 agatgccaaa gtggtcgttg gttcagtcac aggtccgcaa ggctacacgt tctttgccat
    13621 ttatcttgat gtggagctgc cagctggaat ccctctttgt gctactggcg tcgcactcta
    13681 tggtatggtt ggcctatttg cacttaaaat ggagccaaac aagaaggctg acgaggagtg
    13741 gtatgagagt caggacggct cgactcctgg ctggtacaaa cgcgatccta ttggtgttac
    13801 cgacttgaag accaaatggg gtccacagaa aagcagccta gcgttaggcg gtgggataac
    13861 tctcggaaca ttgccagaca atggttttac tgtatccgct aaggtgttgt tgattattgt
    13921 atttccaggt cctataattc tgatagaagg aaggccaatc tgctcaaaga gagatcgaag
    13981 ttagacgaag agccgatatt cagagccttg gctgtattag acggtcggga aggttcattt
    14041 ctttttggct tagatgacta aataattgag caacagtctt ctcgaaccat caaacaaatc
    14101 attaggatcc acattggcaa catccatgtt cgcatattct ttctaatcga tataagtttt
    14161 ttggcgtaaa atccatccgt taacttagca atcgtcacaa gtcgtcatgc taatcttacc
    14221 ggcgtcagca ttgaacatgt ttggaatgga ggcactttgc agctttgata gaactgttta
    14281 cggcggcgat actaacagaa aaaatagaaa tagaaaatgg tggaatgaac ttcgagaaga
    14341 gcgacattga gactgagaga acataaaatt cgatacttct cagctgccta ttcaaactac
    14401 attcgaacgc ttgtcactgc tcgcaagtga acatctagca ctggatttta tggaatcgac
    14461 actatcgtaa acctctaatc ctagagttcg caagatttca cgagattcag aattatctgg
    14521 gatatttctt attctgaagt aatcagtacc atcgtggaag attgcaaaga gaccagaaat
    14581 ggcagcacct gaaaatatcg atttgagttt attgtaattc ttatcattac ataaagattt
    14641 tagagcataa acgccttctt tttgtttctt tctgttaagc tttagtctac ctatatctat
    14701 gaagttagta cttggcatat cgtgatacgg ctgacttgtc atcaacgtac tgaattcatc
    14761 catcaatggc tttcccggca actttatatt cctagtacaa tcgcagaatt cgcactccat
    14821 agtacattgg tccctaaagc gtgcgtcttt tactactgag atattttcga aatactcatt
    14881 taagactgga atctccattg ccatagggta tagtccacct tccaaacagt agcatatttc
    14941 aatgaaatgt actacgtcat cttcatcaat agtagcgttt cttaatgcag attgcattcc
    15001 ttcgtaaaag atctttccgc tagctagaga agcagttttt atacccttgg ctagtcggtc
    15061 tttttcaaca ggatttaact cacatttaag atagtaacgc acatacaata ttaaccataa
    15121 aaacatttta aggcttcgag ttcaatttca aatcttgaga acggccacta ttccccatct
    15181 tgattaggtt gttttttgtt ctttagatac ttgaacgggg atctctattg ctgtcgtctt
    15241 tcgatcgaat ctaatctagt cgttaacgcg ataagctgat ctgataaatg cgctatagca
    15301 tcatctttca ttttgtcacg atctcatcgt tggtttagtt gttctaattc ttctaccttg
    15361 ctctgtatgt ccgccccttg tctttctagt tgatgaatat tcaattcgca agcacatgga
    15421 tgaagtcaga gcaaagtgtg gaaatgatga aaatatggga cgactttaca aacaatcttt
    15481 ggatctactt accccaggaa ccaaattcat cataggggaa ttcaaccact taattgtcta
    15541 gactttctga gaaacgctgc tgaagcaaaa gcagatacca aggctaaata cctgggtctc
    15601 gcggaaggtc aggaacttgt tgaatcagtg gttataagga gacaatggtc aaagcgaaag
    15661 taattccctt gaattaacga caatggtgtt aattcgagat taaaacgcag ttttctaggc
    15721 tgtaatttag taccctcagg tattctcatc tttgagtttc aaagtagagc attgatgcat
    15781 tcaaatggca atcaaccaga gacattaccc tgattaaata gaccaaggat aattcatatt
    15841 ggtcgatcaa atctgtaggt atagttgggc gcttaatcca attcctgtta aaatataagc
    15901 aaatgactta ctaattaatt attctcaaca tgcctacata atagaaacta gtcgtttaag
    15961 caataactgc tggtacgtca aggattgacg gatcttcgaa attacggtca gattcttctg
    16021 cgatgttttc tagacaaaga ttgaagaaaa aattcattta cccggcacag gtgatattag
    16081 gtcataccgc ttgtcctcac cagggagaaa tgaacttatt gaagctgcaa attcagcatt
    16141 aaaaaatgca tatagcccat attcaaagtt tagagtaggt gctgctatta gatctaagga
    16201 aggaaagata ttcactggtt gtaatataga aaacgcctct tatagcctag ccgtgtgcgc
    16261 tgagagagtt gcgttattta aggcagtatc agaaggatac aagtcatttg actctattgc
    16321 tatctcttct tccggtgtta aaccagcttt tccatgcgga gcgtgtagac aagtattgat
    16381 ggaatttaat cctgatttga gaatctattt agatcaagta ccaacgaatt accaattatc
    16441 ggacttgatc tctcatccgt tctcacgaga tcaaatgata ttaagcgaaa cggacagaaa
    16501 ggtcctagga actaggtatc ttgaattact ttgtgagaaa acacaagaaa accaaggcaa
    16561 acgatttaga ttctcaaaaa ttgaagttta tgggagaaac agacttgatg catataacag
    16621 agatatagtg attcctctaa taatacagaa actaagacaa ctaggctata tagaagaata
    16681 tgaaaacgat gaaatcagtc ttactccatt gggtagaaaa aattgtggta agcaagtagt
    16741 agtcgacgaa atcatataat tgtatatgca tcgtaaacga aatattatct accttgcatc
    16801 aatggataat tgatgcagta caaattacga cgagcagctg ctgtcccaca ttgaaagtat
    16861 ctgctccaaa cacatcatct gtacgagaat ctctgcaacg ccaaatggtg gaataagctg
    16921 tccattcccg gagcctacag cgacaacaac aacatgcggg agaatgacaa gactaagaaa
    16981 gagtgaagaa ataaaatgtt ctaagatctt atacgtatct aaatttaaac tcaaacgagt
    17041 atttcataaa gctattggaa tgaaaaatta catttttgta caacaccctt agctagacga
    17101 aatggttact gattaagata gtaacaagac aaagaaatta tggtagtagt ccaagcgaac
    17161 ttctacttac tagctccgtt tcactttctt aactatctct tcgcatagtt ctccatgctt
    17221 gcgaattcta ttgcagtata ggcttcattc ccaactaccc atgtattatg tcctggtgga
    17281 atataagcaa cgtctccagg tccgtattct tcttcacttc catcattcat cttgactctc
    17341 acttttcctg atacgacata gtgtgtatga gaagcttgac agctttcggt tttagcaata
    17401 ggctttatac atttttccca gctccatcca ggttcaagat acattctgct tatggtagta
    17461 tcaccaattt ttgtaagatc aactttccct ttgtcgaagg tccgtgtttc gtctggtgaa
    17521 ttgatactct ttttctgcat ttttgttgta gttcccatat ggaattctac ggtaggcgtc
    17581 tactaatctg tatcggttac ttagtcactt aggagagttc cgaaagtgag taattcttcc
    17641 aaaccagacc agacactatt ttcttgcttg attctctcaa atacctaata aaaaacaatt
    17701 agtcgataac gagatttgca aatcttagcg agatgtatat gatcttacac atattatctt
    17761 ccctctgtga ctaatgctcc aatcctttct aacgatccat cttttacaag ccccttctcg
    17821 attggtttgt agaaatatct ctgagaattc aaaatacagg tctatatttg tagaaatatt
    17881 gtagatgatg tgaggcatat ttgtccttgc tgccgaatgc agctgaggtt aacgccaagt
    17941 agtaaagggt aaaaagatag agaattaggc aaaggaaaaa aggtgtgaaa aaaacatgaa
    18001 acgttttaaa aggctggtct ttgctgggat gcaatacgtc aggataacta atggtcgctt
    18061 taagacggcg ataagggctt aagaatgcaa atgtgcgaaa ggcaaaacgc ctcaagggtt
    18121 ttgcattgat gaaaagtgtg agaatccaca atacacagta aatacaacag tttgggaaac
    18181 taaagaagac atggatgcat actatgcaag cgatagggac ttctcataag atgttctgaa
    18241 aaagtcgacc ataagtgaga cgtttcttgc agcacaaatg aatctgtttg tgaggtacaa
    18301 gcccatttcg gtttgggtgg caactatact cggataatgc atctttatat catagtcgaa
    18361 gtaaaagaat gcaagacgta atgacatcat ccgtctcttc ttctatcgat tatcctccgg
    18421 gaccccactc aattttacct aacaaattac tgcggcgatt cctgcatgac ccaatcaaga
    18481 ccttaatgga aatcacacgt acatatgggg atgtatctca ttttaaattt ggaaggcagc
    18541 agcatatcta cctactcaat aatccgcgtt acatagaaga tgtattggtt aaaaaccaca
    18601 aaaattttac gaaaaataaa acgcggcaaa cgacaaagcg cctcttagga gaggggcttg
    18661 tcactagtga gggagaattt catgatcgcc aaagacgaat aattcaacct acattccatc
    18721 ccaatcgaat aaagacttac ggcgagatta tgattactag tgcagcacgt atgtgtcaac
    18781 aatgggagga tggaattact ttggatatac ataaagagat gatgcatgta acattggcaa
    18841 tcattagtaa atctgtccta ggttccgata ttaaatcaca agacgaagtt ggtaatgcac
    18901 ttctaacttg tatggaatac tctaatcgtg tcctaatgcc ttttggtcag ctaatatcaa
    18961 agatacctat tctaccagtc aataaaggct atcaacgcgc aaaaaagaaa ctagatttaa
    19021 tagtatatag tatgattaaa gaacaccggg acaaggaaag taagggtacg tctaatgcgg
    19081 acttactaga tactctactg caagctcaag atgcagaagc tggaattgga agaatgaatg
    19141 attcacaatt aaaagatgaa gtaatgacaa tttttgttgc aggccatgaa acaacggcca
    19201 atgcactcac ttggacattt tatttactat cgcagcatcc aactgaagag gctcgtgtct
    19261 atgaagaatt atgttctgta cttggtgata aagatgacaa aagaattccg actgttgaag
    19321 atataccaaa attggaatat actgaaaagg tctttagaga atccatgagg ctataccctc
    19381 cagtgtggac tattgcccgc caagctataa atgattacaa ggttgataaa tatgttctac
    19441 ccgccggctc gattatttta atgagtcaat atgtaatgca tcatgatccg catcactttt
    19501 ctgacccaga tctcttttat ccagatcggt ggacgaaaga agcaaagtta cagatcccta
    19561 gatttagcta ttttccattt ggtggaggta tcagaggctg tgtaggtgaa ccttttgcat
    19621 ggatggaagg aatattacta atagctacaa tctgccggga gtggaagatg catcatgatc
    19681 cagatcataa ggtagagtta aagccactcg ttacgttacg tcctaaatat gggatgagta
    19741 tgacgctaga acgtagaaag gaaaggtagt aaccgtagat cctctgctaa gattccaagt
    19801 atttccatct gtcctctgta aatctgggtt acagcctata ttaccattaa aacgtgaaca
    19861 tcacatataa gataaagtag aagatatccg aattgtacgg cttggatatt cgcgttccat
    19921 gcatagctaa tacgaaataa gttcattttg tatgacctct aactgttcct tagtttcggg
    19981 ctaaacgtgg caaaaataga tctggaggga taatagcaat ctcctctatt gaaagatttg
    20041 gtccatatgt aaatagagag acaaaaaatc ttggtgcaga ttttctctga tcatggttgc
    20101 catcaagttc aacagtcatt gactatcata tactagtggt catacgtatc ttacgtttcc
    20161 acctgtgagg gtgttatatc ctatcgatat atcgcgcttg tatacactga agtctgtttg
    20221 ggtcgttttc gatttatgat atcagtagta tctcccgtat gtaatctctt atagatatcg
    20281 caccatttac atgatttatc ttggctgctg caaatggaaa taactttgcg gaaaactaac
    20341 gttaacgatg ttgaacagtt gctgtttgat ggaaactagc gtagcagcct acaggatgtc
    20401 aaattattcc ctgccaggga atttgaattg gctgtaggtg gattttctga gtctgtaatg
    20461 tcgtagttta ccgtgataat aaatgagacg cttctgtcag aggtgtaaat gtattcggtc
    20521 tatggttcac aggtgatctc atttcactta cacttacata tattggataa tagtagcgtc
    20581 ttttggctat tcatagtcaa tatggaattg aaggcatatg agcgccggct ggttcgttgt
    20641 acatcccacc tgtcatgagg ttaagttgct ggactaactt gagtcctttg tctagacacc
    20701 aacgaagcaa ctgagtgttt cgtgtgggga tcagaatacc cggtcctcca taggaaacgg
    20761 ctgaagcaat cagagcctta aggtcgtcat tcgtcaaacc tacagagtgg ttaaagaaag
    20821 ccatgccaaa ttgtaacttg gaattcctga aggagttatc cacatccatt ttttgttttg
    20881 cattctaaca ctatggttac ctgatactgg cgatgaaaaa cccatcctat acaaatcttt
    20941 aacctattta caagagtctt tttattgttg acgtcagttt tattgatgga aatcaataca
    21001 atgcaaacgt aataggcaga gatcctatta atgatactat agttttaaga atagccgaga
    21061 atcttacaaa acctcttcaa ccgctagaat ttggaaactc atctaatgtg agtagcctca
    21121 tacttgctac ttgctactta ggagctagca atgtcaagcc aaacggctta gtaactgaat
    21181 tgcaagtatc tgcaagtaac tctgtatatc tttgtatatc ataattgaca gcattagtag
    21241 tcttgtcatc aaagattaat tcagtaggta tagctctttt ctctagtttg gatgagtgct
    21301 tttgataata atcaactatg atatattgca aagcctctcc tgctttaaga gatcttcctt
    21361 cataacttag gttcattatt gcattattct caatagtgtt tctatctgga tattcgtcag
    21421 aattcttgga aattcgtttg gtaaaaatta attcttcaaa tggaacttta ttttctttaa
    21481 gaagtctagc atacttatga aaaatgcctt taacagcagg tattgatttt cttatctcct
    21541 tgaaatcgtt tcctatagcc attaattcta aaatttcatt ttggcattta tagaaaatag
    21601 ggggagtatc atgtcttctt gcttctattc cccttatttt caagctgcca tcttcaaatg
    21661 ctccgaaata acggtttgcc accggcaatt ggcagtttat tttagagttt aaaaatacaa
    21721 tccatttgta tattccttca aacgatatag cgaagcccac tttttgttct agtacgtcct
    21781 ttagtttgaa ataattatca tggtattctg gtgatactgt cgttggtgta ccgaccgtag
    21841 tagctagacc atctctcttc ttcaaccata ttgaatccac aataccatga agaactgtaa
    21901 atccatattt ttcagctatc tttacagttt gtaaaagaat ttgtctatca aatgcacata
    21961 ccgcgatatg ggcatcaatt cttccaaatt tcgcattatt aaatccaaga tatccaaatg
    22021 aagttactag tatccatttt aaagaggact gtcgtgcgtc ataaatggct tttaatttag
    22081 gattagaagc agcattattt tttaactcct tgtatttttc acgcttatct aatacaatct
    22141 tcaaagatgt tggaataatt ccgattttct tttcgcagat atgataatct aactctggta
    22201 ctctcattct agaatcttga caacagcaat tacaaagtag agtctcagcg gataaattct
    22261 tttttaacat aatatttgga taaagcgaaa caaagtcaaa ttcagcaacc tgttcatgta
    22321 ctcctatttc aggttcaaag ataaatccac ctctatctgc gacgagcaga tcctcaaagg
    22381 tcttaaagtg ttctgccaac gctggcttcc aaggtattaa aatctctttt tgtgtggcat
    22441 aataagactg caagctactc aaacactttc ctatggaagc tctagcagct gtatgtagtg
    22501 acattctgca aattcttgta atttcgtata aaccttctaa tccagaatcg ttcaaaataa
    22561 atgagttgtt ggtatcaaaa tgtattctac caaataacct gacagtagtt ggtttaaagt
    22621 atatttttcc atatgaaaaa taagatgtac cgtctttgtg atttctcttc aatggtattg
    22681 ttggttctct tcccagaatt aatgctgtac cattttgttc agctctatga attagataag
    22741 gaaatgtaaa tgaatcgcca tcatctgtaa atataaaatc tggatcaatc ttctttgtta
    22801 tttccgtttt aagctcttca atgatatcta cttcagattc actttcaatt tcaatagatt
    22861 gagaaaaccc ttcgtcttgt ttgttgtttt tagataatat tatcgagcta attctatcac
    22921 tatatctttg tatcttcctc tctttcctca attttacttt caagtagatg cttctaaaat
    22981 ctggtacttt gtagtctgtt gaccaagcat tatctttgag aacccagctt agtttagagc
    23041 catgatgacg gttataatta gtattgccaa ccttagaaaa tgcaagtgga aaaatatcat
    23101 gttcataaaa gtaagattgt tcaggaggta agtccacatt atagaaacgt aatttgtcga
    23161 agatattgcc cttagcacat aatttttcaa ttcctcgtgc cagaattggt gcttttgttg
    23221 ggtctgaaag tgttaatttt aatatttcat gttttctaca gtctgtaatt ttctcgtaac
    23281 gcgatatgaa ttcatgatct ttaatcaaag aaatgatctt ttcatccttg gtattcaata
    23341 ttgaataaag atcagattta tcatctgatg caacataaat cgaagcactc caagttgtat
    23401 cctctagtct tacagtagtg ccattatgtt gttttatcca aaacaccaat ttgtcagcta
    23461 atgcataaca atcaaacaac caaccagata acatattcta cgggcatctc cactcttttt
    23521 ctactaacgc tggttagcat tggtgtaata cggtattgta cctaataaat tagaattaat
    23581 aagaatatag gattctcgaa gatttatcta taatattttg tcgaacaaaa atcagataga
    23641 ttaacagtct tgggaggcaa tggctgtgag aggctctatt ccaaaattat tgttgttagg
    23701 agccattatg aagccggcaa gatgtagtcc tactaatgct cccctgtcgc taggaactct
    23761 aaatggaaaa gttgtaacgt cccctgcatg gtgctaaatt tgacattatg acaggctttt
    23821 tgcacgtcgg gcactaagaa tctgtaaatg gggagtttcg gtttatgttg aaannnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnngagtttc ggtttatgtt gaaattatct cctctattta cttatgttgt gaaagaatga
    24061 cacgggaatt cgacagagat atcgaataac atgaccaatc agtacaattc agattgccac
    24121 acaatttgcg tttgagatgt aagcaaaccc actactaact aattattctc aacacactta
    24181 cttataacaa agtatttcgc cttatcaata agataagaat ccgcatgtga gtttgtcttg
    24241 ttgtttttat gttttgtata catgaatcaa tttgacgtaa aggactaact ccgaaaaacg
    24301 atggtttttg gctgtcgaaa ttacgaaatc aaaattcggt agtaccgtcc atatattgag
    24361 tcttttagtt tggctcggct gctttaaaaa tggatgaact ggaaatgtaa aaaaagttgg
    24421 gagcattggc aaagcgctag agataaaata caagtgatat taggctctaa t
//
LOCUS       DAOR01000084            4545 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_133557, whole genome shotgun sequence.
ACCESSION   DAOR01000084 DAOR01000000
VERSION     DAOR01000084.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_133557"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4088..4408
                     /estimated_length=321
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1405 a          765 c          774 g         1280 t
ORIGIN      
        1 atctatgtaa gtactacaac taggtatgtt gacagcaact gatagatatt aaattgtata
       61 tgttacctcg cttcagacag gcagtactaa catattccaa tctgtgttca gaaaatctat
      121 gaataactaa aacaaaaggt cgaatagaag aaatacgtaa atgcagaaga agaaggtgaa
      181 ttagatctac tcattgtagc ttgaagcaag gtcaagcaga tttggttgct gcgagatcaa
      241 gagggagaag tgcttcagaa tgggctatgg tggtaagcat gactctcgaa gatattgagg
      301 attcctggat catacagcaa gatttttgga ggttggagca caaaaatgac aggattattc
      361 gagacacaag tacatcgaac atgtccaaat gggccgcgtg ttattttgac ctagattgtt
      421 tgcacttacg gatatagaaa ctgcttccac gaagtaacga gaggaaaaaa caaggattca
      481 tttacaaaga gaatctaaac gttttccgta ccgagtgtga tttagtatga atatttatgg
      541 ctgtattgta taaccgagtg gtggtagtgc gacaacaata atgataccag atgggaatgg
      601 aaatgcaatg tcctttatac caaaagcggg ttttcacccc agatggattg gctgcacaca
      661 tggatgcaca tccgatgcgc gtgtgcgaac aatatgaagt caatttgtca gaatgataat
      721 gaaaaaagaa gaattagaac aagacgctac gatttagacg gcctaacgag tgcctaaaaa
      781 ctatctacct gaagcggtac aagttttacc ccgggctcta aacgcgagcg aatttggtta
      841 cggaactgaa cacattagtt ccagaaaagc tcagagcacg aaatgcgaga agacttggaa
      901 gaaggtgtga aggatgaata atctgataag tttacactat atagtaagga cgaagaggcg
      961 ttcgagtgat agagataaac ccttttatgc taaatctgcg atataaagat tgtaggtaat
     1021 aatctaatct tttgttgata tacgtgctgt atcttcacgc attagtgttg aaggctctct
     1081 agtcgatcta ttcacttaat tcgtagaatt atcgggagag gtagtcggcc atacacaaaa
     1141 atccagttgc tcatcaacca cgatttcacg ctaaatggat tgctaagggc ctaacgccag
     1201 tcaaaaatca gatccatacg gagtagtaga tttcttcaac tccagtactg agtaatcttt
     1261 caattctata aagaatcgtt tgctgtcctt ttgtacaacg acgtaatttg atccagatcc
     1321 tacgatgtta ccgccaacaa actccttcaa ctgtaccata tcatgtgtgc ctacataccc
     1381 tgtatatata aaacttaaga tctgattatg ctataggagg tagttcaacc aaatctacat
     1441 atttgattat ttgttcgaac acacacctag acatttcatt atagacaaaa ttgaaaagga
     1501 tgtaaataac gggaaacgat tgaaccaggt tctgtggaat gtaagacgaa ttgttatgct
     1561 attctatatt tccatcaaac attgggaaag cggtattgat atcttcttcc aaattgatct
     1621 tcaaaaatag ttcttaatgt tgtagaccaa ttctgaggaa catcttcaat cattttagtc
     1681 cattggacat tgtctccaca ataagctgca catactccga atgattgtcc cctaacttta
     1741 gacaatttga ctagtccttt catttgtttt tgttcctgtt tatcctccag ctcgagccat
     1801 ccactgtttg ccctaatagt ctcaaattcc gatttcgtag ttcgtatcat agggctaatt
     1861 tctgaatcat cagttgttac tctgcaataa tcaaccttgt cgtcgtaaaa aatcaccatg
     1921 aatttatcac cacagtcgtc tttatagtac ttaattcttg tcgttatact ttaccactat
     1981 catgtcgcct gcaagttatt gatatgtttt atgaaagata aaatgtctat gatcagaaag
     2041 ccaatctata ttgatggtgc ccgttgatat atcttcgcct gtaaatggtt aatgctacga
     2101 taataacata cttccgtgta gtgttaataa ccagtactag gcaatcaaac tcgtagtttt
     2161 tataacaatt ggattacttt atcttctggg tacaaactct tttaatttcg tctcccatac
     2221 gatcctgcta tcaaattctg tatctgtcct aggatctata gaggattcaa ttacaatctc
     2281 ctgaccagtt attttgtcca aatagtcctg catgtctttg taatcagaaa actctctata
     2341 aggtctacct gattctgaga gtccattcat tttatctacg aaatttatag gtgttctaaa
     2401 tgctactcca ataacataat caatgaacca cacatttaca tcttcaataa ggtgaacgta
     2461 ttcggcattt ggttcattga gagatacctc attttccttt atttcgccca tatgtaagat
     2521 acacgtaaga taatcatata catcttatga actgtaaact gtcatcttca aaatattgtt
     2581 aattcaaacc tagtataacg attcactctg agtagatgtc tgaaataacg gagacttcta
     2641 cttcaaccag ggtgttttcg tcctagtctt cgaaaacaac ttatttgagt atccattatc
     2701 atttaataca gccataaatc tatacagcga caataacgac gacaaaatga ctaacagaaa
     2761 tattatattt caaaattttg atgctgcatt agacgaacta aaacgaaagc tatacgacgg
     2821 tgaatactac aatactccaa attcatgtgt atatgtattg tataacatag aaattcacgg
     2881 gtttcagacg ttcgcttctt ctctttgtaa tagttcgtca tcaggagaaa atggtatgtc
     2941 tgagtattcc accacaatta gaatactgga aatcaaaaca agtctagagg aatttgacgg
     3001 acatattaac aaaaaaatta atcagaacct attagaaaac ttatcaaaag aagagtatcg
     3061 taagttgtta atagactatc tagtccattt aggactggag aaaatacaaa cgaatccttt
     3121 ggatgttgtg gctatgcacg agatgcaaag taaagatgta aagagcttta ttaacaaatt
     3181 gcaatacaca ccgcaaaaag tcgacgacct tccactactt ctcgttggtt ggtatcgtat
     3241 tcctccagct attgcaagac tcattgcaaa gaatataatt caagataaaa atatggagat
     3301 ctatacggat aataacgccc agaagtggat aaagttacag tccactctat tataattagt
     3361 ataatcatct tttaataatt atgttgagaa taattagtca ttattgatat ataatgacct
     3421 caataatata aagagactca ctcaatatat ttgaccttct gccatgcccc tacccttaac
     3481 ttacgtacct acatctaatg gctcttcgaa gtttctagag taattatcta acaatcgaac
     3541 attagcaatc catgtcaaaa taaaggacat aacagaagtt ataacatgac gacaattaac
     3601 taaaaagagg ttagttcatt gcatcgttat tgcaccgtaa gttgattcgt ttcgtcaact
     3661 gagtagtttg tagtacccgt agtaggatta gcaggtgtta atccgctaaa gtgtacttca
     3721 tgcttaccag gagcgagagg ttgaagaaat acataccatc cgtcgactac cacctgtgtg
     3781 gtgcctacca atgagcaaaa aaagagaggc tggggttcaa tcctctctta cagaagatga
     3841 tatgaaatca tatttacaag aagtaataaa agaaattcag aatgcacgga aaggtaataa
     3901 taatcatcac aaataatgat ggcaaccagc gtctgatttg tacgtgatca ttatcggaaa
     3961 aattatggct tataagtttg cgcgggatcc atagtatagt tgcaatacat caaaggtcat
     4021 aaaggggtct ggtcatcata ttcgaaaagc atgatgcgca tactgttgta gcaggtttgt
     4081 actctctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ctcacgtagt gcttgatatt ttatttagta
     4441 actgaattat tgctttctag gtaagtagtg aaattacatg ggtagaacaa ttccttcatt
     4501 taggctagcc agtgctgaag aagaaagaga atggaaagta tttcg
//
LOCUS       DAOR01000085           17434 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_54256, whole genome shotgun sequence.
ACCESSION   DAOR01000085 DAOR01000000
VERSION     DAOR01000085.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 17434)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17434)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17434
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_54256"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         5030 a         3597 c         3460 g         5347 t
ORIGIN      
        1 tgctaccagt tgggtgtcaa tgggtttaat tgaaaacata tcaacaacgc gacaagctat
       61 cccgttattt gatttcaaaa ccttggctgc atctaatgct cttgtaacca ttagaccaca
      121 tgctataatt gtcgcatcgg agccgtcccg caagatgttt cctttgccta tcctgaaaat
      181 cgcgttgtct tcatatagaa cttcactcaa tggcctcgca agtctaacat aaaatggccc
      241 aggtatcatg gctattgatc ggatcaaata ctttgctgcc acggcgtcag cagggacgat
      301 tactcgcata ttgggtatca ccctcatcgt cgaaaaatct tcgagctgtt gatgcgacgc
      361 gccgtcaggt cccacagtca gaccggcatg agaaacaaca agcttgacat tcaggtctgg
      421 atagcagatg gtgttgcgga tttgatcaag gcatcgtcct gggagaaatg cagcatatgt
      481 acttgcgaac gcaattttgc ctgcaattgc aaggccagct gccacggaaa cgaggttcgc
      541 ttctgctatg cctacattga agaacctgtc aggataatag tccccaaatt tctttatctt
      601 gagtgaatcc gtggtgtcag cccccacgca gactatattt tgattagatg ctcctaattc
      661 tacaagtgta tcgccgtatg ctcctcgcat atcggcattt ttttgttcat tacttaagag
      721 catgtagttc ttcctctatc cctagctcat ttgctagagg aatcaatata tgtttgcgtg
      781 tcttcaacca gtcgcgtctt atacctatag tgttcgcctg ctctaggagc tttctctcca
      841 acgccacatt taccttgtac cttagctgga taattgcgcc atggatatcc ccactgccat
      901 ataccgcgtt tccggctacg aatattctag ctccagcctc atagactgct tgcaacatag
      961 tagcatcaat gccaccatcg gcttcaatgt accctaagaa tccgtgttct cttagctttt
     1021 gatttattag ccggattttt gataagactt ctgggatgaa tttttggccc gcaaagcctg
     1081 gatttaccga cattattagc acgacatcga catcctgcag gtggggaaaa agccaatttg
     1141 gaagatctgt agatggattt agtgcaatac ctgggctaat tccgttcgaa agtaatctat
     1201 gagtaatctg cgcaaattca ctttcactac atgtctctgc atgaacacta actatatcac
     1261 atctagcatc aatataatct tggatatgag ccaacggatt ttcaatcata agatgagcat
     1321 caaatggtaa tgtggtgata tgtcttagtt gttttattgt atctgaatag aatgtagtat
     1381 taggcacaaa tctaccatcc atgatatcca gatgtatgat gtcggctcct ccccgttctg
     1441 cgcttcttat cttctcttcg taattttctt gctgcccagc tataatagat ggcgcaatta
     1501 ggcattcact tccaagttct tctaacaata aaggagtgtg ttctattggc ggagcctttc
     1561 catgccactg cggattgttc gtcatatgtt tgatgccgtt ccctttaaca gtgtttgcta
     1621 taattatagt cggactatct tttgcctcac ttgctttgtt tagagcgtca attatctgtt
     1681 caacattgtg accagctatc tcgattacat tccaattgaa ggcggaccat ttatcacgag
     1741 tcggatcgag aggcatgata tcttccgtga aaccgtcctg ttggatgcgg ttgcgatcta
     1801 gtatggctac taggttatgc agtttgaact tggcagcagc cattgctgct tcccagatct
     1861 gaccttcgtt tgattctccg tcacctatta gagtataaac acgatttttt ttaccatcca
     1921 gcctatttgc cagcgctatc cctatcgaca ctgaaagacc aattccctcc gagcctcctg
     1981 aaaactcagt tcctggtaca ctttggtgga tctccttctg agtgtatcta accggatgac
     2041 cctgcagtct gctcccgagc ttacgcaaag tcttcatttc agtgggtgga aagtaaccgg
     2101 caatcgccag tgttgcaaag aaccccggag ccgaatgtcc ttttgagttt ataaaatagt
     2161 ccctctcgct ccaatctggc attgttggtg aatgattcat ctttcgaaaa taaagaacgc
     2221 ccatgagctc cgcagctgat aatgatgcgc caggatgacc agagccagct tcagcaattc
     2281 cttgaatagc cagcatacga acgctgcata tcttcctctt aagatcaagg tagcgcattc
     2341 taaaacccat tatgaggatc gcaaccattc aaattttttc agatcatatg aagtgcggcc
     2401 acagggaaaa taaaaagata tatgtaaagg cacccaacca tctctattgc ttccgctatt
     2461 taggagcagt ttacagcgta cggtctttga gataatttaa aataattaaa tgattacaag
     2521 ttagcatggt tggtgttata gtacaagagg ctaagattga ccagatgcaa actgcactcc
     2581 tagtcattgg cattttcgaa aacgaacgtg attttttcaa gtcaaaagga ttgaattcta
     2641 caataaccgc tgcgatcata gagctcatcg aaaataaaga attcaaaaga agttttggat
     2701 caaatataat tttccatatg atgggcaaag gattgataaa aaaaattatg ctaataggtc
     2761 ttggaacgag agaaaaattt acgtatgaaa atgcaagaat tattgccggt aaagcagctc
     2821 tggaggctaa ggaactaggc gtcgaagaat tctgcatttt acccttcatg gatgtggatg
     2881 aagctttaat agaagcgatt tctgaaggtg ttcttctttc gctatatacc ttcaaccgat
     2941 acaagacaag cggaaaggaa gctactacta tccctgcgca ggctacaatc gtggttaatt
     3001 cagattctgc gaaattccag gctgtggtag acatagtaaa tttgataaca caggcggtca
     3061 attatgcgcg tgatttaagt aacctaccgc ctaatgagtg ccccccttca caaatagcca
     3121 gtcttgcgtt ggcacttgat ggtgaatacg ggataaaaac tcggatttta gaacggtatg
     3181 agatggaatc tatcggacta aacgggatag tttcagtggg aaaaggaagc aataatccgc
     3241 ctaaattgat aattttggaa tattatggaa atgtggatca tcaaaaacca tatcttttgg
     3301 ttggaaaggc tgttacgttc gacacgggag gcatatcctt gaaacctagt gataaaatgg
     3361 atgaaatgaa atttgataaa tgtggaggct gcagcgttct cggaatacta aaagcaatag
     3421 catcgttggg tcttccagta aatgttatag ggataatccc ttcggttgaa aatatgcctt
     3481 ccagcacttc gtacaggcct ggggacataa ttcggatgta caatggcaag accgtggaga
     3541 tcttgaacac ggatgcggag ggcagaatta ttctcgcgga tgcaatggca tacggtgttg
     3601 caatattcaa tcctcaggca attatcgatc tagccactct tactggtgcg tgcgttattg
     3661 ctcttggtgc taacgttgca gctgcaatag ggaccagcaa gcacctcgcg gagagattga
     3721 gcaaaatatc tgaaaaaaca ggagaaagaa tatggggtct cccattacat gacgaatact
     3781 atgaacagat aaagagtact gtagctgatc ttaagaatat cggcggtaga ccaggtggtg
     3841 cgattacagc agcagcattt ttgtccaatt tcacgaacga tattccttgg gtacacttag
     3901 atattgcagg aaccgcatgg actcaagaag gaacgtttga gcgaagctac aatcctaagg
     3961 gcgcaacagg atttggtgtt cgcactgtgg taaaatttct gatggaggct cctaataagt
     4021 aacgttaaat ggttcatgtc cacgttgttg ttcgaaatga ttgttttacg ccacaaaagc
     4081 aaagtattac taaatgcgag tcaagttggt gattgtatat atttcgatgt ccatccaggt
     4141 attaatgtct aacttttttc agacaaaaat tggaaaataa gatgttttag atgtaaaatc
     4201 cactttgacg aacttaactc gcttcgtcgc tgcatcttca ttaaaatatg gatcaaatca
     4261 agcgccagca ctgctgcttc ccaaccgctc acgcaggtaa gtaacacagc agcgaagaca
     4321 ggtttgactt ccgggttcgg aatgggaccg ggtcgcaccc tgcctctatg accggcttac
     4381 tagtattagt gtcagagggt caaattaacc ttgctctaga tcgcctgtta tatcacagat
     4441 tactttattc agcacctatt aaacctaata gatcaaaccc ttcagcagat tttgcctcaa
     4501 attgcacaat tgagatcaag tgctcgtcag gatctacaaa tatggcatgt tttccaaacg
     4561 gttcttctct aggttctttg aaaaatttta cattcttttc ttttaatcgt ttaacagtag
     4621 tgtcaagatc gtttaccata aaacctacca gtatagttgg agcagctttt gttttttctt
     4681 tcttttttgc cgggtgtaaa gccaaaatgg taccattgct gaaaaattca gtccaatctt
     4741 ccgattggtg cttcaaagga agttcgagaa tacctttata gaattctata gattttttca
     4801 tatctgccac taagagaatt atcgctccca gcttggtgag catcactaag ttacaccgtt
     4861 ttgaatttac gtaggccgca ttaagaactt atcgctaagg gaacaaaaat gacttggaaa
     4921 acacatcata atcataatca taataataaa catcatcatg actgaaatac tcgagtgtaa
     4981 tattgtaata ttgcattata tacatctacg ttaaaaatac atgcgtgcac cctgaaaaac
     5041 tgttcttcat ctacgttgca aaaaacgaag aatgggaaaa aaggcagagt gaggattggg
     5101 attacgtttc atcgatgtct agattttacc gctggtggat taaacgcaat tttgagctag
     5161 atttcaacgt ggaagctgaa atcctacctg taattccggg taagctgttt gatagaatgt
     5221 ccataagtta tcttagtagg gatcacgttg aacgtggtaa atcaatttac catttttact
     5281 tgacatactt caagcctttc tggacagact gtcagaccga aggatatgcc gcggaaaatt
     5341 ttggaatgat ttattggaag agactggaag cgcctgtttc agactctaag cgagttaagt
     5401 tctttgctga tatgaactgc gcaaagctct ctcatgtgtt atgtcacgaa gtgttacgaa
     5461 tgctagggaa aaagagaaag gaatattttg attcaatcca tgatctgtgg gatcgtcatg
     5521 tttacaagga tctaccgtac atttactaca atgaacgatt tagaatagtc ctcaaggaaa
     5581 gttcctatcg atatgtaacg attgatgtaa agcatattca ttgacgatat agagaacaat
     5641 tatttatctc tgcacagaca ggtatctagc tgagtttaac attgttcata aaattcgggg
     5701 ctattctctc ttcggtcgga gccatattac tcgttaccct agcggccatt ttacttgtct
     5761 atcctattgt caagaactgt catgaactga aatgcacgtt tacttcttct gcgcccagcc
     5821 cccttaactc tttcgttaaa ccggctttcc tagcaacctc tctcgtcata attgcagcag
     5881 gagtagcatt aattagattt ggatcatggt atcaatacaa gtctagaaag gcagaaaatg
     5941 gtatgccata gtatctggac aagataaacc tgagcaaatc cataacgttc aaacatcgtc
     6001 atgtggaatg ttaaaaagct agttacagcc tacttaataa ctcgggtgaa gttggagtga
     6061 ctgaatatga tagtggtgca tactctgtgc attttgctca ttttgcagcc aagttggaag
     6121 ctcatctgat cagattcggt gtaacgtgcg cagatgcgga ttcaatcata gaggaaagtt
     6181 ctatcattta ttttgaaaag ttaggttctg caaagaaaaa gcttttgaag tttgtcagaa
     6241 aggaggatcc cgcaaaagtc tttgttgatt cagcttaccg agcaatcgaa agacacatac
     6301 cagaagcaaa taatagtttt ggttcccata tcgaactgtc aaagtgcatc catcaaactc
     6361 attaatgtgt gtatactgcc gccctaaagt agatgctgct atgatgaagt ggggccgaag
     6421 ggatttatcc tgaccaagcg cagacttata gtcaattgaa cccttgaccc acgggtttct
     6481 tctaatccta gccttttatt cgttagatct ggagcccgtt gcgatgccgg gcttcgctac
     6541 gaccccacaa tggcatccgg taaatatcat gatgatgtat ctctataagt cttccgagct
     6601 tgttccagga tagggcgtct tacttcgagt tgtttcagaa agagataaat aattttggga
     6661 tatatcaacg acattgactg gagagactca gcttcgggct agacggacaa gaatcaaaat
     6721 cttgcgggcg atcgcggata agaacggatc ggcatgtttc tcagaaatta gaaatgcgac
     6781 cggcctttct accggttcca tctattacca tctggacagg atggaaaatt atgttaataa
     6841 ggactcaaaa cattatacta ttactcagga gggcttacaa atgcttcgcg aacttgatag
     6901 caaatcttcc tcttctccga cctataagac ccatgaggtt ccatttggtg tctctgaggc
     6961 tcagattgga cggaatgtca agcagattgg tcccgtcgga gcattcatta gtgaatattc
     7021 atcggtattc gttcttggac tggttattat ccttgtcccc gtcggcttat ttgagaattt
     7081 ccatataatt tttcccaact tatccacagc cgcgaaaatg atcgcaaatg ccaccatgat
     7141 ctcttcgctt tccatcgctg cgctcctttc tatttctttt gtagtgatgt taaaaagaca
     7201 aattcttccc aacggataca gaggaataat gctatctgca ttaacagtat tggcggtttt
     7261 ggtggttaac attctaatct tttctggtct tgggacacaa atcgggatga gttcctttac
     7321 attctaatca gcaataaaga atagagatta tatcaacgat attgtgttaa gtaatagtcc
     7381 gagtttacta cttaaagcac tagatcaagt ttcgattatt ctttctccag tggagatcaa
     7441 ttctccaact tggttataga gctgtacaaa acgcgtaccc ttctgcgtta tcctgtagat
     7501 ccgttctgct ttgctgtatt ctattagacc attttcaagt agtacggcta aatactcctt
     7561 taattgagca taggaaagaa aggctctata cattattttt gtcttcgctg caccaccatt
     7621 cgctgcttcc aaaatcagtg ccgctatatc agttctactc ctatatttca ctagacaaag
     7681 agaacgttgt atgccgataa aaggacattg agtaattgct gtagtaaatt tttctatcaa
     7741 atggatcaga tccggcatcc tgctgtagtc tctaattgaa gactacctgc tgctcattaa
     7801 agataaatat aatatgcttc ataatttatt gttgactatc tgggatttct tgtttacatt
     7861 tcccacgcca cgtcattggc gggaagtcag atttcatact tagatcacca tcttctagac
     7921 gcgaacctgt ttgtagtata tcctttttcg ctcatagcta gtgagacaaa acgccatttt
     7981 ttgtctaatc ccttcgacgt tccaatcctt ttggttgtta ttatacctgg aggcttgact
     8041 ccaaattcta tgtgtatatc actcttcgga tctgtcatat cttcgccatt caatgactgt
     8101 tggatattaa gtgcttgact aagcttccca ggtccagatg tcaatgataa ttcttcacaa
     8161 tttctacgaa aaattttcat ccgcctaacg ccctctactg gttgtaatgc tctgattagt
     8221 acagcaccag cttcaatttc aggaggcttt gctgaaacat tcaagcaaaa atgattaccg
     8281 taaataaagt aaatataggc tctaccaggt tctccgaaca ttacagcgtt tctaggcgtt
     8341 aaacctttaa atgcatgact ggcagcatct tcgttaaagc cgtaagcctc cgtttcaata
     8401 attatgccac agagtcgctt gggcttttcc tgaccacggg agattatacg gaccactttg
     8461 ttaccaagca attccttggc cacaagctca gtagatcgat cataaaaagg gcgatccgga
     8521 caaatcataa gtaaccctgt ttgctacaag taaaatgtaa cgacatctgc tagcggcctt
     8581 tagtcagtat cttaggcact tgaaataaat tttactaatc atgagccacc gcttgagatt
     8641 tttgacgcca atgggtccga ccataatagg tctaactgtc taactcatct atggtgcagt
     8701 tctgtggacc atacggcctt tacaggccaa ttttcggtac tacttcatga ctcctagttt
     8761 cgtttaacta tttttcatac tttggtacaa cacatattta gaattgaact agattccgat
     8821 attctcggta tctcatctga acatcatctt gggtaaccga gagaagtctt ctaatgccaa
     8881 aatcctcaac agcaaatgag cccattacat ttccatatac tactgcttcc ttcatcgtat
     8941 tgaaggtcag actgttttgc tttgcaatat atcctatgaa gccgcctgca aaagcgtctc
     9001 cggcgccggt aggatccacg atctcttcta atggatatgc tggagcagga aacactatat
     9061 tttctgcgat caataatgat ccatgttctc cttttttaat aattacaaac cgtggccccc
     9121 acgaaattat tgtttttgcg cattttacta ggtttgtgtt tttacatagt aatctggcct
     9181 catcgtcatt aattactact ccatctacag cactcatcat ctttactaca ttatcgtggc
     9241 agttcaagat ccagtattct attgtgtcac agactactag tttaggatcg gagaagtatt
     9301 ctagtatctt catattttga cgtggatcgt tgttggctag ataaatatat tcagacttca
     9361 tatattcatc gggaattact ggttcgaatt gtgcgatcac attcaactcc gtcttattgg
     9421 aggttctatg agatagatcg taatcgaagg aagaatcata atggaaagtt tttccaccat
     9481 tctcagtgac aatacctctt gtatcaactc gcttgtcaat gatggttcgg tactctgctg
     9541 gaaaatctga cccaactatg ccaactattg aagtattggt gaacatgctg gaggaaaggg
     9601 atgcgaaggt ggctgctccc cccaaaatct ttgtttcagt cttgaaagga gtacgagtag
     9661 tgtctaaggc tatcgttccg aacacggtta acatatgtct gcagaagttt attcatcaaa
     9721 actaataaat tttaagaaaa acaaacttcc ccgtaaagtt tgcatacaaa agattactcc
     9781 gaaaacatag tggttaataa cgagtgtaac cgatctcctg acgacgaatg cgattaatag
     9841 tggcaataac aggcagttca ggtgtaatct acgggatcaa agtgttggag gctctactta
     9901 aaataaagat cgagacccat ttgataatta gccaatgggg agaaagaaac gttcaaatcg
     9961 aaaccgatag atctcttgaa tctgtgaagt ctcttgccac tagacattac aacaatgaca
    10021 acatggccgc tccaatctcc agcggctcct ttaaaactgg cgggatggcc attgttccgt
    10081 gtagtatgaa aaccttgtct agtattgcaa atggatatga cgataatctg gtttctcgcg
    10141 cagccggagt ctgcattaag gaatcaagga ggctagtgat tgttccaaga gaaacacccc
    10201 tatcaaagat tcatttgcaa aatatgacaa ggcttgcaga catcggagtc atagttttgc
    10261 ctgcgatgcc aggattttat cacagaccaa aatccatgga tgacctgata gctcatatcg
    10321 taggcaagat cctagaccag tttggcatcg aacacaatat tttcacgaga tggggagaag
    10381 cgggttgacc gtaacatttc ttggcaacta ataaaccagg cagttttaat gattactctg
    10441 ttaggactta tatgctaatt tcacgattga tccatatggt cgggctcatg gtaatctcaa
    10501 gggatttatt acactttcta tagtacagac tagcggacgg tggtatggaa tagcacgata
    10561 cacaaaaaaa tactctacag atcgttcaat ggattttttc attcctctgt tgagtttgct
    10621 gtttctcggg gtaatgtata tcttaataac acgaataaac caaggttatt ctgggttcct
    10681 actggtttcg gcagcgtctg ttttgatgat ttactgggtt agagaattga aacgaacagt
    10741 gaaagacgag gattcaaaac cattaccagg agatggtgac acaaaggact gggtttatga
    10801 tctgataaag aataaaaatg aaatggtatt tgtagccgaa gtccctggac cagaagaaca
    10861 gattgatatt aggttaacaa ttggaaccct tcacgtgaaa agtggtcaga atttttccaa
    10921 gactattcca ctcgacctaa ttgaagagat gaatatatcg gattttaaat atcgaaatgg
    10981 catacttacc ataaaaatca acaaggtttg attattgaga attttcttcc cataatttca
    11041 ttttatcaaa ctttgccttt cgtcttgctg cttgcagcat gttgtacaca cttttgtgtg
    11101 agctaccctt agagagcatg gatattgcct cgatgccaat tctaatttct tgaaatgagc
    11161 cgatcaaacc aacagtatgt ccatatactg agacgtatgc cccagtcaac tcttctatcg
    11221 ttttcctgga ctttcctgct ttgcctataa ttcgtccttt gattctctgc atcgaattgg
    11281 ctgatttacc tgcataatct ctaatatcta tgagctgcag caaatcctcg gccagtaagc
    11341 gatatgcacg ttcgggtgaa aatcccttcg aaatggccaa aattatttct atcgctttga
    11401 agggttccgc attggttgag ggagatgggc cggaagaaac gagggcatca ccattctcgc
    11461 catctatttc aattatgaca ttgcattttt gctcaatttc ttctttcact ctcccccctt
    11521 tgccgataat tacacctatt ctatcttgag gaattttgat tatttgttca aaactcattt
    11581 tcaatatctg tatcctggtc ggatctagtg ttaaccttta cgcatttgtt atccatactg
    11641 gaatttataa atcatcaaga aaggtactcc tgatattctt tttatactcg cttcttttgc
    11701 aagtttcaat tttattgcac gatcaacaat tctgtggcca actaaattcg caatcgaaca
    11761 atccaacaat agtttttccg cgactaaatc ttcaattatc tcttgttgaa agtattcttt
    11821 tgtaagattg atgacatatt tatcttgttc aatttgtgtc cctatcaatt ccaagtcgca
    11881 aatattgaac attgttgacc cttgatagcc catcctcctc atcgcatatt ttccgccgac
    11941 tgctccaatt ctggacaagc actgagaggt aagtgtaaac gcagatttaa atgagtgtgt
    12001 tgccttgcga actattccaa gtcatttttc cccaagaaaa accatccagg cttacgattc
    12061 attcgtcaac tctaaatgat gacgtcccat cgttgtttag atagtctgat tccaaaaaga
    12121 gggaagatag gttaagctat tttattttga ataatactgg caatgccatg tatttactag
    12181 tggcaacaat tattccaatg gttgctattg caaatactgc aacagcaacg gttgcgaatt
    12241 ccggcactat gtgagtaccc gtgatttcga ttaccgatgt accgttatcg aagccaacca
    12301 tcaacgttct tgactgtgcg ttagttctta tttcgtcgtc taccgcatac tgaccgtctt
    12361 caaatactgc gaagttgtcg tctacatttc cttgcttttt agagtctata acatcccggg
    12421 gaagttcaat aattaatttt ccatttgatg tagatgagac gtttacgaga agggtcgtat
    12481 tgtccttctc cgccgagatg ccagcgagct taccacctgt aatctgatac tttatgggat
    12541 atgctttatt acctaacttc aagttgataa tagcgttgct ggccgtcacg gaagcctgat
    12601 tatttaattc tttatttgct ccgatggtat tttgcgcgta agcgagttgg aaagatatga
    12661 taatcacaga tattgtggca agttcgatca cagataggac tattgccact gcctttgtag
    12721 cttggcattt ttttcctttt atttcgtcga actgccttct ttctgtcaat tatgaatatc
    12781 taccttctaa aactttgtgt ggccttaaaa ctcttgtgct atctatggaa ctaacgtagc
    12841 gtgatttcca atcgttgatt tacaataatg ccttttgtcg gcataccttc tctctcttta
    12901 atctctttag tttctttaat cagcatggtg gcatttgaga tgccctcgtt cgataccttc
    12961 tgtactccat caacataccg aaaatgataa acatcactac tgccaaggca tcgacaccgc
    13021 cggcggccgc ataaatcgtc tatgtactca ttgaaattaa tatctcgaac caaaagtact
    13081 tcggttgttg catattttta agtacctctc tacggcaaag atagaatatg cgtagatagt
    13141 tgcctcaatt ggacatagtt gccctgtcgt cgaatcgtta cttaaaaatt aaagtgtttt
    13201 tctttaacaa catccttatc tgaaaaataa ccatagttac tataaggcaa ataataggaa
    13261 cactacatga tatcaatttg gtggtcgata ttggctctct cgcctctaca gcaacttctg
    13321 taggctttgg aggcattact ggttttctaa ttgggtacgc aataaaaaaa gtcgtaaagg
    13381 tcatattggt gatagtggga ttagtatttg ttgcgtttgt gtttctcgaa taccaaaaac
    13441 taataaccgt aaactggaac aagattcaag caagtactat cgcggccttt ggaaatgcaa
    13501 cgtcgggcca acttcttgca cgcagcggca ttcctggcac cgatcatatc gcctcttcaa
    13561 tgagcaattt cggcatacct ttaaccggta gcctcactgc cgggttggtt ttaggcttca
    13621 taagaggtta ggattactct gcatagaatg ctagatactg gagacatatt caaggaaata
    13681 acagctggac tttagatgac actaagaact taccgcaatc agctataata ccttagtgtc
    13741 attgtccaat tctataataa aatctatttc tactgcacta tcgagtggaa gactactcgt
    13801 tccgacagcg actctgcaat gcttcccttt ttcaccgaaa agctctacaa gaaaatccga
    13861 tgcaccgtta ataacttcag ggtggctact aaaagatgca tcgctattga caaagccgga
    13921 taatttgacg aaccgtttta tatcgtcaag attatcatgt gcatctttta tatgcgctag
    13981 tgcgtttatg gtgcagagtt ttgctgcctg cttgccatca catatagaaa tgtctctgcc
    14041 cacctttccc ttgaatttga cttttagagg atttgaacct agttcaatag gcaattgacc
    14101 agacacaaac acgagacttc cagttttgac tactggctca taagagcctg tagtcttcgg
    14161 aggcctttgc aatgttatgc caagtaaatt tagcttttct tctatcatat ttttaatcgt
    14221 agcttcatac tgttattaat ttaataacag atcttggaat atatggattg gttagctaat
    14281 cgacccattt gtatcgaaga cattatcaaa gcaaatatct ttaaaagcaa taaaacaatt
    14341 cccggtaata tgggagcaga tcatgatgag cgatcacttc gcaggtacaa caacctcatc
    14401 cctgttggtt ggacgattct agttatgggg atttcgcttg ctacggtagt aattttatgg
    14461 gtatggtttg gacatatagg accaacctac gcttcagaca ctttaataca ccaacagaaa
    14521 acgttaagac agcattatgg cctgccacct cagcctgcca ttaccgatcc taaggcactc
    14581 ctcactcccc cctcgcttag agacgttaag aataatactg tgaacagcaa caaacaaacc
    14641 tagacaattt cggtaagatt ctacaaatag cagttcatcg atgttcgtaa ctacggctca
    14701 atggaaggca ttctatcaga aaattcctca aagtgacgga ccagagtaag ttacttctta
    14761 gtccagatca acttaatgaa attgccgaat aaatagggtt agcctcttag aaagaccttt
    14821 cagtcggata tggtattgta gaatattttt agctgacttc gctaatttag cttgcatctt
    14881 agattaagct gcagatcatt taatgtttta tatgcaggtt gatagtgaag acatcaatac
    14941 ctcacgacca ataacatgtg agacccacaa taaagtatgc cggctaatta gcactactaa
    15001 caaatgccct tgttgtgaca aacggatctg cctttgcgac tataccctct agaaacgaat
    15061 gttattaaga gatgacagat gacagttagg aattaaggtc aatgtaatgg gtactcttaa
    15121 caacgaaagt tgccagctta ttcagttaac tttcaataga gttccttgag tacttaatta
    15181 ggaaaaaaaa tgagaacaca attctcccgc catttagtat gtccacaaca tactgataaa
    15241 caaattcgtc tttcattaga gtctcttcaa ggcaaagata atgaatgtga agagggcttt
    15301 cttaaatgcg atacatgtca aacaatgtac ccaatagtag aaggagtagc gatagtagta
    15361 aaaaactttg taaaatacgc cgagtgtcgt gtggcaactt tcggcaactt gatactgaat
    15421 agcaaaacgg acaagatgaa aaactttctc aaagcctatg gagggcagat tacgtcaggg
    15481 caagccatgg acgatcgata tgaagaagga ggaccctggt ttgttcccta tcgatgggtt
    15541 caatatgatc attcgtctga ggatagactt ctaagtacac tcaaatggca cctaaagccg
    15601 aatgaattct ataacagaat cgtgcatgca gtcagcccaa aaacggatgg agttgctctt
    15661 gatatggggt gttcgatggg atatagtaca ctacagctgg caaaaaagta tgcgtttgta
    15721 gttggtattg atctatcctt ctcctttatc aaggaggcca ggagaagatt ggtgacgtcg
    15781 caccaagaaa atgttgagtt ttgtgtagca gattcattgc acgcgccatt taattatatg
    15841 aaattcgacc tgatactagc acttaattta ttagaatttg tccaagcgac tgacctacta
    15901 tcatctatcc actggctatt aaagcctcat gcgctagtct tattcgctga cccttacgat
    15961 tataaccgac agtcacaccc aagtcaaaaa gttgatgccc agaccttcag ggccatggta
    16021 gagaattcag gttttgaatt agcgcaaaag actaagacag aatcccatat accgtggata
    16081 ataaaaataa atgaaagagc ttatttgttt tattttgtag actacgttat ggcgaaaaaa
    16141 ctttcaaaac ataaagtcta gtaatatttc attcattata gaagaaataa gtcaaggtgg
    16201 tgcttaaact aaatttcctt cacagtcaat ccattgggca tttatgctaa tttttcgtat
    16261 tgggaattta tgcccatgaa tccgccaaat gccaagaact cgatcgtctc ttcgccgtta
    16321 tttgttatgg tatgctccgt tctttctgga atatagatag tctcagtctc tgaacagact
    16381 ttcttcgtcc cctattctga tcttgccctt tccgttgata atataataca actcttcatg
    16441 atcctggtgc tgatgtgttt cgtaggaaag agtgggctgt aagtgagcta aagaaacgta
    16501 cgtgatcgct ttaaggcact tatatttttc ttcaacctca gcatctatgt cgccgctggt
    16561 tttgttaacc gtagaaagta atctccaatc gaccccgctc ttgtgtgcta tagtaggata
    16621 aacatctctc cagtttctac cgactatcac actaattgta aggtcgcaat catataacta
    16681 cataactagg acaataattc taagttttcc gagtaattct aagcatcctc atctacacgc
    16741 gataacagct acaaataacg aagattgcta caagcatttg gaacagacta gcacctatgc
    16801 tagtatacaa cattcgagga ggtgaacata ttttgttaag atgtagacgg tctaaaaaca
    16861 tgttcaaatt tcgtttgggt ttgtattttg ctcatggtac ataaataaga tttgaaagat
    16921 ctagcaggag cgaatatgac caggctgaga ctaggatttt ttcgttggaa gcctgttaat
    16981 atcatgtcat tgtccgtata gaaggttcat gcagtttgaa tgtaaacatt cataaattat
    17041 aattatcgag tttcctcgtt cgtgcaaaga gattcgcaaa cgctagaggc gaaaagggct
    17101 ttcgggctac tttttatagc cgtatccgtc gcagtagtcg ttggcacgct tcttagtgaa
    17161 gtcgcagcca aatataacgc tccaatatat tatattgcac tagtttggct ggctagtttc
    17221 ggtgttacat ttggagcaaa ttttcggaaa tttcgtagag tgattgtttc tgtacgcact
    17281 cgaatgagaa atagtatgaa atggccttct agtgcaaagg ctataaatgg actatgttgg
    17341 gctgctccct tcgctttgat aggcgtgttt ccgtcaattt actagcacct gatcctctta
    17401 gacacagact taggtaattt ttctagatat ctac
//
LOCUS       DAOR01000086           11670 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_43241, whole genome shotgun sequence.
ACCESSION   DAOR01000086 DAOR01000000
VERSION     DAOR01000086.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11670)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_43241"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    291..505
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3639 a         2126 c         2167 g         3523 t
ORIGIN      
        1 atcatatcta atatagtaaa gtaattacat ataaagtata tagtaagtta taactaaccc
       61 ctactacaaa accaactgga gctgcatgaa ctctaagcat ttgaagggaa ctcattagac
      121 tcaataaagt atctatagga aattgagtca tatgatattt attatatact gtgattggta
      181 cttcttgttg ccgccttcca tctcactgta agtagctcat atttttagcg tgatagaggc
      241 ttcacagtaa tggttatctt aaaacatgtc aacaagtctt tacatctact nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnncaagt ctttacatct actgcggatc tcaaattcgg
      541 taacaccagc aagacttgac atgggtagac aagacttgac tcctaacaat attgctgcga
      601 agtatagata tgccgcaact agtccgttgg gagatttact atcggcaaga agatgattgt
      661 tagacttgtt agctatttca gttgctaact tcctaactct agaatcaatc cttgtggatt
      721 tgacaagatt tgagatgtag tgatcaattg ctaattttat tggaatcacc tagtcagcat
      781 ggtaggaggt agtagaatag aattatttcg aaaatctatt atgcactgta gaaaatgttg
      841 agttaacgtg tcttctcatg actagaagat gaattatgct atatattcga agtggtcata
      901 acaatttact atgattagaa ataatttttg gtctcgcttt tacttcccgc cttgaaataa
      961 tcttaaaaat gcaatgttca ttcttaacta tttaggaatt ataaatgcaa tatgagtaga
     1021 attgttcata gattttcaaa tacctgcact aaggaaatgc aaagcgtttt ttccctcatc
     1081 agttaaggag ataatatgat gtgttccaac cttagtttca gtaataaatc tccaatactt
     1141 ttgcaatggc tctatcagat tcttatttag ggacatatag gctgcttgtt cactgtattg
     1201 ttcttttgtc ctctttctat tggttattct tttttcatcc tcctggatga ggccagcttc
     1261 tattgctgag tcctttaaat cgcgtttacg gacaccactc ttcccagcac tttttcgatt
     1321 tattaaatct agacacctta tcaatttatc actagggatc tcaatcttat actccggaag
     1381 accaataatt tctttaagac cttcagtttc ttgtttctct tccttcgcca cagagctggt
     1441 atatctttca ggaactgcgt aatagggttt tatcgtagca gtatctttaa acatcataca
     1501 cgccatcatt gaagcaatgg cctgaatctt actacccaca gacacattta caagaatgga
     1561 gttggctttt tcttttaaaa ttattatcct tagagctctt agagtatcaa aaagatccgt
     1621 tctatcagct tcggtttgca gacattctat tcttgcctgc tttaacttat cttgtattga
     1681 tgagacaaat tgatttccca tgtcaacact aggattactg tgtgtgataa gccacacttt
     1741 gtccgctttc attccgagtg cgggtaagac tatccgatca acttcaaaac caactggagc
     1801 tacatgaacc ctaagcattt gaagtgaact catcagactc gatttgctca ttaaagtatc
     1861 tatagtaact ttagtaaatc gatatctatt atatactttg ttattggaag gtaagaagac
     1921 aaaaaaagtg agacgagcta gactaacaca agacgtacta acgttagaaa aactgccgac
     1981 cgaagcagct gcgacaggca tagataacca actacaagaa tcgttagatt gtccagattg
     2041 ttcggacatg atgctccagt tatatgattg ggataaaatg ggatacctat gcgaaaattg
     2101 tggactagct attgctaatc ctgctattct ttctacgtat gttatctcag gagtaaggac
     2161 ttgaagatca gccttgttac gtttatatat aataaaatag atggtacggt actatacggt
     2221 aacattgtct tcgggcaatg agctgaaaag gaagcaaaca gaatggtttc atcaaaagaa
     2281 gacggttttg cattagcctc gagaatagac acgcaagcaa gcgccaatga tattatccac
     2341 aaacttgaat cgcccactat accgcgcata agtgccatag ccaatatggc atggtgtgag
     2401 cgtgcagcat atggtatatc gtttctgggt gtgcaaagta attatttacc aggcgcagga
     2461 gatatcggca gcgcagtcca tagaattgta ataaaatcaa cgttggaaat agtgcagtcc
     2521 ataaaaaact gcacgaggat ttgcaagtcg gaagcgatgg aaattttttc aacaaacgcc
     2581 aaagaagagg tcgatgtcaa ttggaaattc tatatgcttt ctggtataga acagccatta
     2641 ccgatcataa tggaagattt gaaaattaga gctgatagac ttgcaaatca gctaattacc
     2701 catcataatg atagttacca aaaattacta ctaagacccg agtttacaat acgcaatcca
     2761 aagattccgt tagaaggaag gttagattta ctgaaaatta gacttgggga accagttcca
     2821 gattcttgta aatacataac aaccgatgat ttggttaatt taaacataga agatatcgag
     2881 atagtgcaaa taaaaacagg caaggcgaag ccaagaacac ctaggatata cttgcaagct
     2941 gacgccgaag ctttgctgtt aatggaaaca ttaaaactga aacaaccacc cagttacaca
     3001 tggcagtttg cagataaaga tgtgaagcat aaatcattca agttcgctaa ggtccatcaa
     3061 gcaattgata aattcatcag attatggaaa gcacaggaat ctccagctat aacaggatac
     3121 tgcccgactt gtccactaaa aaatgcttgt ttagattggc attttgcaag aagcgacaag
     3181 ctatcctttg aggatctggc cagaagaaga gctgcgttta atctctcaag gaaaatacgg
     3241 gaggaaattt cagatacaga cagatggaaa gtttatgtct ctcttaggaa tgcagaagag
     3301 aggcatgcgg acggctgggc tttaacgggc ctaaaattgg acacatccga aatcgatcca
     3361 atcaatcaac agatcgtttt gagctctgcg gaaaaccggc catttggtaa tttcgtcgat
     3421 tttagtgttg gagattttgt gaccgtgtct gacggtaatc ccaacctggg aagcaaccca
     3481 actgctataa ttactgatat agatcttgaa agatcgtcag taaagctaca atctatcaga
     3541 aacgatctgt atgttctcac ccatgataac aggcaaacgt ccaggttcac aatggaccga
     3601 tttgatttcg ataaggggct tacgacgatt agatatcttg atgatttctt tcggcaatct
     3661 tcttatgctg acagcgtcct tggccaggga gctgccctag cagcaacagt gacatgagga
     3721 cctgaaatag gagatagcga gagacgtatg gctacagtaa attttgacat aaattccctt
     3781 acctttaacc agtcagagaa tcatgaacag cgtgctgcca gggacagaat aaaactagag
     3841 agcactattc caataactgg acctcctggt actggcaaat caactgtaat ttcgagttgt
     3901 tgttttgatc tcgtcaacga gtattctcca gttttggttg ttacccccac aaactctatg
     3961 gtcaatagta tcttatcgaa gattgatagt cttgcacgct gttcaaattt acaattgcca
     4021 agaggtttca taattcggta tgggaatgta acggaactca actacgctta tcctcatttg
     4081 aatagctata ctttagatag cctggtatta cagcatcaag ctaacaatgg cggtggcatt
     4141 agcagaatcc gagcaggtat tgatctgatg caaaatgcaa ggataatttt atgtactgat
     4201 tatatagcca aagatctgaa aaatattatc caagctggtg ccgtcttgat agatgaagca
     4261 ggcctggtta gccttgacaa aatggggaca atcttttcgt cattgagaga caacaatgga
     4321 aaagtgattg tgataggaga tgataaacaa ctcccccctc cctcacgtga ttatgtggcc
     4381 acaagcctct ttcggtcgat actcaaccac tttaattcga cattgcttag aaaggagtat
     4441 agattcaatc gggatattct tgatttaatt aatccatatt acgacaataa gctcgttgca
     4501 gatcattctg ttcaggatat ttcgacggct gagatagcta aaaaagatta tggcggcatt
     4561 aacgataatc ttcgaaaaat attgcaacat gatagaaaaa ttgtatttgt tgatactaat
     4621 gatgggagaa gagaagacaa gcattttgta aatagtggag aggtatccat aattaaagac
     4681 atcatgaagg gttgtcaaag tatgggtata gacaacatta tggtcatcac tccatacaaa
     4741 caacaggaaa gaatgttaca attgcaactt cagcagcaga aagtgcaagg cattcgcatt
     4801 ggcactatag ataagtttca gggacaggag gccgaggtag caataatatc aatggtaagg
     4861 tcgaacactt gtcaagatta tcatgaggca ataggttttc tcaataactc aagggcatgt
     4921 gttgcatttt ccagaggtaa acgcaagacg ataatagtgg gagatcagac tactctaact
     4981 aagaacaagt ttctcgcaag ttcaattgat accattacaa gaaaagatgg attttttatt
     5041 tggagaaatt ccaactgatt aaacttccta tgtttcgcag tggttttttg ttataaaaat
     5101 taatgcaaat taaaaacact tttaatcagt cataaaattc gagttattat acattgaata
     5161 agttgaaggg gaaagtttct gatgcgacta tgtgcacaat gcctttgtga tctcagaatg
     5221 atttcaattg aacttagagc aaccgatctg caatctatct agccaactag acgttgaacc
     5281 atgtcaaaat aggttagttt gaattgctga caaagatttc agaaacattt caaaatctca
     5341 acaaattatt tatcaacagc acttcggctt aatgctgtat atataagagc tgtaggaacg
     5401 tccatcagca ttgagcaccg cttactgcca agtaaatatt ttatacttta tttccacaaa
     5461 cttagcgggc aaaagaagac tttaaaagtt gcagaataag cctcgtagat atgcgagata
     5521 ttgataacct gaaaagtctc tacaaaggcg tgaacgggaa gtgcaaaaag aatgttcgat
     5581 ttgttggtgt atatttgtca tccccgttat ttcgctcgtt attctacttg tctttctggt
     5641 cttcgtcctc tcttgtttga tgtcttttaa attcgtctaa tagatgtctc gtttttgtgt
     5701 taagaatatc atacattatc aatccaaaac cagcggctcc acatacaatt acaacggcat
     5761 atgtcactgc agcaatgcta agccccgact cagcacttgg actctggaaa taccctgtta
     5821 atataatact cataacaaat gagatacaaa gaagaattat gccgcctatt gaaagctttc
     5881 gtttgtaaaa tgcaggcact gacgtgactc agtggatgag aattactata tttattcata
     5941 ctcgctacac atttgaacta acattttctc gatgctttct taactattgt tttccactcg
     6001 ttctcgcctt caattattat catgttctca gcaggaatct tgctttcaaa gctccatgtg
     6061 gcccaaaata attatgacac atagtgcttt gaacttgtac atggagattt cagagttgtc
     6121 gtgtttacat ccatattgag ccaagattgt gtattctgag ggtgaagacg gtgaaagcaa
     6181 tgaggacagt ataaccccgt tacctaagca agtaatgatc acgaaattgg aagtatacaa
     6241 ctcccacacc gttttcagta atttatttca cgttccggag tctagtctaa taactacgaa
     6301 ataacagtac cctatccact gtagaaccta cacatttctt gaatcttaga cttttacata
     6361 ctttttagct atctcttcaa ctttatccag gagttcctga gatggccttg gaattttatt
     6421 attatcatct aatctccagt atctgccatt atgatagact aaaggtttga tgttctcatc
     6481 tgacgagatt tcagttactt ctccaacaaa cattatatga tctccaagac cttcctgctt
     6541 tacaagcttg cattctgcgt tcatcgcggc tcctcttatc attaaggttt tgatactctt
     6601 tgctttatag aatattatac caagctcttt aaaaagagat atcttatcaa gttcttttcc
     6661 tgtatatcgt cccccaagac tacataatat attttggtct tcagctgcta gattgactcc
     6721 aaattcttta gattgattga tgttttcagc tgtggcatcg tcaccacgaa cgttgatagc
     6781 aataagacta ggactgtaag atacatgatg ggtccattcc gctgccatca cactcggacc
     6841 acatcgccca tcagtagtaa tgagacccac attggtcaaa aattgccttg tccgcctatc
     6901 gttccacgaa agttccatat tttggaagta ctaggtatgt gtttagaata taaaggacgc
     6961 taggggttag aagcaattcc actacccata tttggcaacg taagagaaat aatgaaatta
     7021 actaataaaa gaaatttgca gttgatacca atcctagact taaaaggggc aaccccgctg
     7081 ttatcaagct ccttttactt ccgctatgca ggaagtcaag atattccaat aattgcttac
     7141 aaattttcga cctagcccaa tgcaaataag ggatatgaca tggtgaggga tgacctagac
     7201 aagattcacg aagatgacca aggaagttac atgtataaca cacagtttga agattttcga
     7261 aaccgtcttg cgacctttat ttccgggtgc aaagtagtct acatctacat ctagagtgtt
     7321 ttcctcgtct gtttcttact acaataatac aaataatcta aatttgtttt tcttccataa
     7381 ttcaaatctt tgaacaatcg attcagatcg ataatatatt tgaaatcgtg tatgatgctt
     7441 ctgagttgaa cgatttacca aaactcttgc aagcaatcac tccaacgctg gattcacggt
     7501 aaccgttttc tccggtggcg aaggaatgca aacactcgag gccgaaattg cgcttaaaga
     7561 ctatcgatgc gaatgttgca atcatagttg aacccatcat attttgacta aggaattgac
     7621 ggcgtccttt cctttatcgt gttgagaaaa tcttctagga cttttacgta atcgctgtat
     7681 aattgcttat ggaacttact tccaattgga cagtaagcag gtttattttc tgcttgagcg
     7741 tgactaggat tgtgacaaag cccgttctct gtttttttct tgagatcaat agtcaaccac
     7801 gaaagcgctg ttacagccaa catgaacaga gttgtttcag tgtgcataaa gctgtattga
     7861 gtaagttctt catctttact actacactct ttttgagtag gatttgcaga atattcacga
     7921 ctctttagaa gaacgttgat tgccagggaa gaccagaaga agccgttcgg tattatctta
     7981 ttgtcagctg ctccatacgg aggaccaaat tgcccaaata gttcctctac agacgaatag
     8041 aaatcctccg gattattaaa gtgcctgtta aaccatatct catctgtcat agatttcatg
     8101 ataattttga ttctattaaa catagtcttg tggagaataa tagactatga tgagtttgag
     8161 tgattggcta gaccaaaccg gtttttggca aaaaccgatg tatgcaagaa atactttatc
     8221 cccagatttc ataatatatt ttgagattat tataggcttg catatggtgt gaacgtgcct
     8281 atggagggat ttccttttct gtattgcagt cttccagcga gttcagtatg gatcatcggt
     8341 cagaatatta taattattgt tgttattttg gagttaggag ccacattttc agttgcctct
     8401 agaggaaaat caatagattt gactataggt ttacaaaggt ggtaagaaat actgtaaaag
     8461 atgcgaagtt tacttttact atgaaaggat attctgtcct tgttgtggta tggcattgag
     8521 ggccgtttcc aaccagtaaa aggataaaga aaggcggagg caattacaac taagaaggaa
     8581 agaagaacaa gatagaataa tcaggattat aaaggaaaca aagaagtagt ctgtcgaatt
     8641 attatcttga aatggtcgca ttctaaaaga caagaatttt tgattccaac tagtattcga
     8701 gtgagaataa aagaccctac gacgattttt cagattataa actttttaat ccaaggtggg
     8761 cataaggaac tctagaaccc tagagatttt cgctttattc ttggaagtcc tttcaaagac
     8821 gatatatctt tagggttcta attatataaa atttgcgatt ataatctaaa tagttacatc
     8881 ggactgaagg gtaaggctat tcacttacct aaagcggtta gactaacgaa tttcttggtg
     8941 gatattccgc tcctcagtca actatattat ttgtcaaagt cattaatatg atttccccca
     9001 ttgtagcaat tggtgctttt ttaacgaata aagtggtaaa gggatcagaa ttacaagaag
     9061 tagtatgctc gaagaggatg ataatattaa aagagaaatc atgcttcaag ttttaaggaa
     9121 cactattatc ttgaacggag aaaagctcaa aattccagcg tgcatcacac ctactgctgt
     9181 gcacgaatta gaatgtgttt tgatatatga atgtaaatcg actcacggtc taacagatga
     9241 acaaatagac gagatcaaaa tgaacgctag aaaagacatg ggaacgaact agaaaaaatc
     9301 aaacgaaagg gagattttat ctgaaatgtt aatgaacttt ttttattctg ctattaaaat
     9361 cacaactcaa accgaaatca atgtcacgaa gacttaaggg cgcggccgac ttgttaccaa
     9421 tgaaaatatg agattcatag ttcgtgcgaa aattcctaca gaagcgggaa acaaaatgat
     9481 tcaagatcca aattttctaa agaatcttga agactatgtg aacaaagtca agcccgaagc
     9541 atcatacttc tttgaggctg acggcgatag agtagcagtc ttcgtagtag atatgcaaag
     9601 cacagatcag ataccctcgt tagctgaacc gttgtttcaa tggatgggtg ccaaggtaga
     9661 atttcacccg gtaatgagcc ttaacgattt gaaaaaagca atctcaggag caaagtgatc
     9721 atagaaaaag ccggttatta tagttcacta gtgcaataat gattgatatt cctcttcttt
     9781 tttcacacct tgtttttatt ctacgcctac agtaatcatg tgatatattg tatctttgtt
     9841 agttacctgt ataatctgta taatctgctt agttgagtaa ggttattggg aacacagacg
     9901 atttattcgg actcaaaggc aatccgaaca agttcacggc atgagaatgc aacacaactg
     9961 gaagaagttg caaagactat aatccaagct atatcaattg aaaagccgga atttagatat
    10021 gtagtcggca acgatgctgt tagtttattg gaagcaaaac aaaatatgcc atattcagag
    10081 ttccaaaaga tgattataca aaatatcaaa taatagttct gcttatcatc aaatatctgc
    10141 agacgatata cagaatacaa cccaagaata atattcctcc aatttaagag gtctaataat
    10201 aagtaccaaa tcaatcctcg gtattaaggg gactcgactg attacgcgtg aataacgcta
    10261 ttgaactaac ggctagcggc cacttctggg atatcaccaa aatagttaac aacaaagcaa
    10321 ggttactcca aattgaacgc tattccgtct ccggactttc gaagaaagat tgcagtatga
    10381 tggttttgta aaagttcttt atatgacatg taactaaggt cgtcgttaat tggtttatta
    10441 gatgataaaa ggccctttgt agccttactt gttatcttcg tatcaaaaaa cgcagctaat
    10501 atgtcatctt tagaattttc gtctgcgtaa agaaaggcga catgcatatg tgatggaatt
    10561 ttacgaacaa attcaagtgt ttcaacagca cgaatatgtt ttagaatatc acttattgcg
    10621 agtcgtggat tacgaatgtc gctgattgta aaatttgggt ctatcaatac agcgtgtacc
    10681 agcaattctc ttttaagatc atgaaggatt acctctgctc cttttctgag tactctgtat
    10741 aatgactttt caaataagtc ataattcgta agtaattcct tttctaataa ttcattaatt
    10801 gaacaaatat ggtctaacaa ggctctagaa gctgattctc caagtcggtc gattgatttc
    10861 ttggctgcat cataaagaat cgcctcagct ttagaagatt gcgggaagta tcctagctca
    10921 tctaagacgt tcgaatactg ctgaaattgc acgtccttat ctgagcgact atcttctacc
    10981 agctgcgttt accaatttgt ataagcgtta tctttcttat ttaagctaag cagtcgcata
    11041 ttcgaacagg ttagttagat tttcaactac ttgtggtgat gttatgtcag aataagatca
    11101 taccttctaa tttagagtga agactcgctt cctctttttt cacattgctt tatttcatga
    11161 aattttgctt gatcgtttta cggaaatctt catcagacat acttcttttt gcttccatcc
    11221 atgtttccat atcctttcca gctaaatacc tcagactagg attttcactt gtaacagctt
    11281 taagcactac ttttgctaca aggtcaggag atgacgcatt ttctcgtata gaagctaata
    11341 ctgcatttaa ttttttcatg atctccaagt agggagaatt tggattttga gatttcttgg
    11401 tagcaaccat tccatcagca aagtttgttc taataggtcc aggttctacc aagaccactc
    11461 ttattccaaa tggctccaat tcatatacaa tagattcact cagaccttcg attgcaaatt
    11521 ttgtgctaac ataagcggat tctccgggat aaccaaatct acccgctgct gaactaatat
    11581 ttatgatgat accagatttc tgttttctca tgataggaag gaccgcttga gttactctta
    11641 taacaccaaa tagattggtt tcgaactgag
//
LOCUS       DAOR01000087           14532 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21125, whole genome shotgun sequence.
ACCESSION   DAOR01000087 DAOR01000000
VERSION     DAOR01000087.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14532)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14532
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21125"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4266..4323
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4779..4995
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3938 a         2887 c         2441 g         4991 t
ORIGIN      
        1 gttaattgct tccgaacgat attttgttgc agtatattgc actgtcacac aaaattaatg
       61 cacaaatagt tttgtgaata aacacaatca gtaagaataa cttgtactag caagagaggc
      121 tcagaattat ttcccaagga gcattctaat gcaatttaat gtaaatggca acaacacaga
      181 actttagcgt ttgaaggctt ggtttgcaat tatctgatta attgtttgat agtcatcttc
      241 tccaaacctt tcatttatcg gaataattct atcatgataa tttgaaaagt atttactaat
      301 gacttcatcg gcatttggaa taacgaggag cattcttttg ttgctgcctt tatcaaagtt
      361 tgtggtgaaa atatttcgta tcactggatc tctaaacgag tggcctataa caatccatat
      421 tcgtttgttt tccaagtcct tgttcaaatt atagaacatt tgtatataag gttccacata
      481 caattgcttt tgtattagtg gatagataac catatcttta tcatattttg agccttttcc
      541 aatttcagta gcaaaatcta ggtgatattc tttttccttt atacactcag tgtctttctc
      601 ttttaaccat gatgttgagc catgaagttt gactagacgg agtttgccac caggtccatt
      661 acaaaccgta tttccagcac aacgcagaaa gaagtctgga tatagacaac cattgccatt
      721 aaagcatgtg tccagagtta cgcctattac atcctctctc caaaaggtct ctagattatt
      781 atcatagtta gtggtgaata aagtccaaga gtaactatat tttaccttca aatccctcga
      841 gttagctggg gcatctccgc ttgcatctgc taaactgtta aagaagtgtg agtatacctc
      901 cttacgcttg ttactgaatt tatcctttag catgcacgat tttctaataa atcgcttaag
      961 ttttgtctca agttgagtaa gtgtatctgt aaagctatcg tcaagatttt tactaaaaaa
     1021 tgatttttca caaatcttcc tgcacgagta gatagcaagc tctccgaaat tgtccacgct
     1081 gtactgtttt aagccatcaa ttactgagaa aactgcttca atgtctacgg aaccgagatc
     1141 ctgttctagt agcttaacga tctcatcata tacccgctgc tcttgttgtg tagccgtttt
     1201 taactcttct ttgaaatcta ctgtcatttc cttcatagtt ggatttccaa agggctttga
     1261 agctcctgct ccaaagaaaa atgagacatc tcctgtcaac actggcatag cgtgtagaaa
     1321 gaaaattaca atctttaaag attgtgagtt ttggtggacg aaacataagt tctaggcttt
     1381 taaatataca tcaatctgtt tagtgagggt cgattgataa tgtattttat gtacctcgct
     1441 cagaattagt tcctgaagaa tttaatgtaa tttaatgtaa acgaccataa tttcgagttc
     1501 tataaacatt cagaaataac catgtattgc aaatctgcta ttcaacaacc attattactt
     1561 cttaagtttg aatgattcta aatggttatc tggtctatct cgctcaaatt ttcgtatttt
     1621 gaatagccct tgccctccca attgtttaaa aatcagattc ggttattata caggcaaggg
     1681 gattcagaag tgatatgtta tagttgccga gaggaggatt tcataatcgt gttcaatagt
     1741 tcaattagta tctaattccc tcgtgaaatc cttaggttta tatcaattaa ctgcttctaa
     1801 ttacctccca gcaccgcttt tcttattctt aaaaactact aaaccgatcg taatcgtatg
     1861 cttgttttag tgcagtatag tgcactggtg tccattttta tgaatgctat cataagaccc
     1921 aaatgctatc aaacttcctt gattgataat gtaatcgtaa ttctgtataa aaaaggggat
     1981 ggagcactta gagcttcata taccctggaa cgaacaaatc agacatcgaa aatccttagt
     2041 gccagtcccg tcttctatca ttaggatctt ttggtgtcgg aagcaaatca ctttttgaac
     2101 ctctaacagc gaattctatg ttatatagtt ggattttagc cattgattaa tatcccctgc
     2161 tcccatcatg tagcctttcc gctcttaact tctgagcata atcctctaag accggaataa
     2221 tcccttaagc ggatggtagt ctccatgcag gtacacttgc tgttgttgaa gaatctgcac
     2281 tcacattggt tattgctatg atattgtaaa ttgtgattgc aactaccaca aaaagaaccg
     2341 ggttcacgtt cagtcgtcgt catcttcgat tattcctcgg ttatcttttg ctgtagtagc
     2401 tacggaatga tgccagtcta gatgttttat gaatttgtca agatcgcatg catatttgga
     2461 acaataataa caatgaagtt taaccttggg tattggctgc tcttcactaa taagactact
     2521 caatcatggc ctccgagaca gctccttaaa tctgtcctgc atcactgcag ctatatttcc
     2581 ttcaccacgt agatcagcca tacatggctc acataggtac aactgcccct cctcttccct
     2641 atccattatt tcctccaaag agtcctcatt atcagggata atgtatattg gctctaatgg
     2701 agtatctttg gacttgcagt tatcacagat ttgttcgctg tgcatatcta tctatgaatc
     2761 tcctgtttaa cactccaatt accatataga atggcatgga ccctattcaa atccggctca
     2821 tatcgaagga tttccttttc taattcctct agctgcttgt tttgatgtag atatatccta
     2881 gctctttcca tatttgactg caagaattcc ttctcctttt gacgttgttt acgtcggctt
     2941 aggtgttttt ctatgtctct aggaaccatt acccatatct cctgcaacaa gttgctcagc
     3001 aaatgttggt ttcttcttac ttgggaactt cttctgcttc caatacgtct ctgccaattt
     3061 gttaatagta tgaccggctc ccatcattcc tgccaattca tcaatagaaa gctccgatag
     3121 aaactcatct aattcagcct tacaagctgt taccagtgga actgtgccct tcgccagctt
     3181 cgccggctca ggtagacctt ctattctgtt gatatcacgt tctattagct caaccacata
     3241 ctgactaaac gatttgtctt tgatatcaaa acgtatcagt ctatctacct tatccatcaa
     3301 ttcctgcttg tctctagggt aggagaaagt agctactata tgcttcccta ccactttctt
     3361 cctacctcca atgaagcaag caaccagcaa tagcaataac tttcctttaa tgaataataa
     3421 taataaataa ataaattaat tattaattgt gttattatta tacgggtttt gagagtggag
     3481 tctatggaga aatcttctat aattatcggg ttaataatta acgaatacat ttctttttgg
     3541 tttctttttg ggattccagt ggatatggtg gttgctcgtt cactcgcaac gatgaaggac
     3601 atagttagga gaacccctca ctacaacatt ctttgcatat caaacataga ctagaagctg
     3661 aatattgccc gttacatacc tggcatgggt atatcttggg tgtttggtca gctaaacagt
     3721 cactacaaaa ccacttgtta ctattttccc ggagaatagt acatacttca caggtggtca
     3781 taccttgacc tcctcagata gtcaccagcc cttagccttc ctacctatga ccggaacgga
     3841 ctctatcttg ccttccttcc ttagtctagc cagcatcttc catattgttg tcgggctaag
     3901 tacggataat tgcacaagct cgccgtaact aacgccagga ttctcttcga ttatcttaac
     3961 taggttattc tcatatttga tcagaaactt cttatgaagt gtttcggtta tgctggctga
     4021 tatcctacat ctctgcactg tgtcctcagg tctccttaca tttgttgtag atggatcata
     4081 gctggcagac gcagtcaaag cgaggggtta gcaccttttc cgttcgtgtg cccttgcaga
     4141 ttttgaacac ggtttatgtc gttctatgcg attcaattac ttactgtctc cctttgctag
     4201 tgacgcttga agcaagtgct gacaaaattt acttctagag aggaagcctc gaaactctcg
     4261 ccagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnntctttgt gtttcgatta gcttgaattg tttttcaggt acagaaattg ttatctgttt
     4381 taccatttgt tgtaatggac ttgagaagaa taatgatact aaggaactaa gtttgataat
     4441 catacgccga attcatccct tccactcttc acaaattttt tgaagtatca aataatttgc
     4501 tatcgtccgt tcgatcgcgc cgacactagc aaatctgtag aatatcattt tcactgtctg
     4561 tctttgtcca ttatcagaaa cgtaggcgtc agttggcatt attactcatt cctcgtcgcc
     4621 gtcgttcctg aagatattcg cgcagttctg taagtacttc ctcaacgtgg cgtatacctt
     4681 cgtcttctgc cactctacgg ctgagtcttt gatctatctc atcattctta ggtcgaattt
     4741 ctggtagttc tgtgacagct gcaagatctt cctgtatann nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnatctt cctgtatgtc ttccttctcc tgttctgtta tttcatctgt
     5041 atacaaattg agctgtgaaa aaaagtcact tattcccggt cgatacaaca cttcgtttgc
     5101 tggaaacgtc acaagatcag ttagatcgta tccaccttcc ggtgcaattt ctttggattg
     5161 aagcatatgc agtatgtact tccagagtcc tgccgcgcca atctgtttaa ctgcaatgat
     5221 tgcatctatt tcagctcgga attcacgttc gtatggactg tgagggtaat agccctctaa
     5281 gatatcttct atgtctaagt tgggatatac gttttcttta agccattcaa gggtttcctt
     5341 cctgaagacc ccaattttaa cagcctttgt tgaattagca atatcatctc ctgtcacatc
     5401 gtcatctact attgtcacaa caattgagcc gttatctagc gtcttttcta tgaatttgat
     5461 aacgtatttt gtgaatcttc cttgtgtgtt gatcagtgca atgccgtact tatcggcata
     5521 tggttttagt acacgtggca caccctcttt ctctataacg atattgattc tagcttttga
     5581 ggctagctca tcaatacttt cataatctat tgggaaggtt tgaccttgat aatacatcat
     5641 gccacgagcc ttggcatata tcccaagatc ttctcttgtg atattttcat caggccataa
     5701 ttcctgacaa cgttgactaa tactatttgc aagggattta caagtagtac cccagttctt
     5761 gcgtggttca aacccttctt tttccatttt aggccgtata cggtagattg cggtacgttg
     5821 cgtaatccca tctaattgtt tcactgaagg atctgcttca acatcctttc gtctctcttc
     5881 tagaaaatca tccaaataat ccgtggctgt cttctctctt acacctgaag gtatgtgtaa
     5941 atgagcatga ccttgtccat gatatgatgg aattttacac gacatagttg atagagcttc
     6001 agcaatcttt gcagcgtatg gagcttgtgg taaactagtc ttacctgctg atccaatcca
     6061 atcaggaacg tttgttcgta agtttatgta aacgacggat ggaacccttc tcctagatag
     6121 agctttatct gtgttaaagc cactttcaga cagaacttct cttgcactat ttttagtatg
     6181 tgaacctttc ctatctgtcc atttgtatgc atacgaataa gcagtgtctt gaaaatatct
     6241 ttctcctccg tctattgcag gagtatcatt gacacaaccg atgaaaatca cttgtcccgc
     6301 actgcttgaa tctaaatctc ctctcggtgt tattgcaatc tcaataccat aaggaaatcg
     6361 ttggcctgta tcctcatttt catgatatcc tgcaattatc ttacactgag cagtcccttc
     6421 attaatttga aaaacatctg caatattatc aactatttct ttttcacgtt cttttttatc
     6481 atatggaaac ttgtattttg tttcagcagg catcttcatt aatgaaacaa atactttttc
     6541 taatatgtca atatcatcta atgcttgttg tacagttaat tgccctaatt tttttggaag
     6601 tataatactt tctctccaac cagcaaacac atctgacaat aaaattgatt tatcagcgat
     6661 tctcttcaga aatcgtccaa gggcagcacg atttccataa tattgtatac tgtctgtcat
     6721 tgcatacttg gtaccacaat actaaggtcg gtccgtatct ttggaatctt gtatatttta
     6781 tagtaccgta taatttgtga gtatatggtt tctttactgt aatcactgtt attgttcgct
     6841 gataatggta atgcaacaga aacctctgta tcggtgttat caattactgc gtcatagttt
     6901 tgattgatta atacttgcgg atctagattt tccttgtcaa ctcgtagaag cgcagtgtat
     6961 tctttccgat tgaatctcaa tatgataggt tcatcccatt gtatctcctg aaaggaatct
     7021 ttattgtcct cccggttctc agtccatttt gcatagccca ttcccagact tctttttaat
     7081 gcatcaccca atgcaccagt accatcttta tgttgattgc gcttcgtgct atcccaaaga
     7141 cgaaaatcaa atgttgattt aaggtcatct aacactggta gattatcgat atttgaatta
     7201 cgtaccgcta tacgaatgcc tatatctata tgccagacac ggagcatgat cttcctgtca
     7261 gacttagcat tattatgata gcattcattg aaccaatcat atgagttatc catcaactct
     7321 ttagctatga ataatggcca ttgatactgc aaatatgatg tctgtgattc aatgcctcta
     7381 acgctaaagt aatccattgc gacaggggtt ttcgttctga ttggcttagg agcagggctg
     7441 ggggaggggt gtcgaggttt tttgacaatt cccttgtctg attcaacatg tctaattcga
     7501 ccattgaaat tgtttggttc atggcttgta tctacgaaag ttgatctagc cactggcctt
     7561 gccttgcctt ttaaattggg ttcctgacct ttttccggtg tcatctttag ctcactccgt
     7621 ggccatgaca ttcgcatcta caaatgaaac agtgacctag aacctcattg cgatatgatc
     7681 caagacaaga tttgcagtcc ctagaaaggc agtgtttgat gatagctgaa tctttaaata
     7741 acgactttcc gtaatagttg ctatccattt aagggaccac ttccttaggt ggacttctgg
     7801 ggatgggttg aaccagtttg tgagtccctt cattgggaga tgactgagac tcttctagaa
     7861 agactgcaca caaattgtca gctatcagac tagtcttaca cgaatcacag aaccatcccc
     7921 ttttgtttag gaagataacc cacagatagt ggataccggc attttgacat cccttgcctg
     7981 cgcattcttt atattcctga tggagccttt gttcggtagg gttcacgtgg tcatcacaac
     8041 ctattgaact ccactctgga cctgctggct ggaagcctcg aaactctcgc cagcaggtat
     8101 cattgcagtt tcttcatctt catgatccgc attaataact tggagtacaa tcttcgtatt
     8161 gtcaactttc aacgcaaaca cggttgatgc attgaggttt tgttctttag ctatctgcgc
     8221 tggaataatt aatgccaacg atttccgatc ctttgtgcca acccaatagg gttgaacaac
     8281 atgtatgtgt ctacccaaca tcacaacatg gataacgtat taatatgttt taaactagca
     8341 gtaatcacag attggtcaat actaacagat tagttgaaac atccattaga attttacgtt
     8401 tacttttaaa caaaaacctg cactataatg agataattcg acagactagt ccagacagaa
     8461 ctcatattga caatacaatt gacgtcttag agagaggaca actaattatg gaatataaga
     8521 tcagcaagcg tgagcgtaaa cagcagagga tacatagtca ggcgaggata atgcagatta
     8581 cagaattagg acgagagtta atagatatgt cggttagttt ggagcagtat agtagtgcat
     8641 attttgaatt aaagaagctc gtggatgaat attttattaa atggagaggc gaacagtcga
     8701 atagtatcag agataatata ttgagaaaaa aaggatttac agatgacgat ttagagttat
     8761 acaatgaatc ttataatggt gccatggtag cattaggtgc gctagtaaca gaaatattcg
     8821 aggttataat cactagatgt gcgttaaggt tgtatagatt taagattact gatgttgcgg
     8881 aggatatact caataagatc attacaaacg cagtgacatt tcgagtctca ctcttgctaa
     8941 aaggcattaa atcacatgaa tctattccat ttaataaaga agatctgaat gcaaaagaac
     9001 aaggtgatgg aagttatgtt agcccatttg ccatacaaaa cctgataatg aaaatgccaa
     9061 tgcaatacta ttttcctagt gtgatgcata aagaggtcaa aaatgcaata ctatcttatc
     9121 tttctctatt aaaaccatcc aaggaaagta tacaatatac ttatgagatg ataaaaaatg
     9181 gttcgaaaga actcggacgg gattatccat ccttgccaca ccatgcgaca tgtttgtttg
     9241 atgtttatga agcctatatg ggtaaatgag attaactctg tgtgtagttg attactcact
     9301 tagttttcct gtatctattc ttgtaagtat ttgttggaat ttattttcca tttcttcacg
     9361 catagctttc atatcttgtt catatttctc ctgtagtgac tttacttcca actctttttc
     9421 ttgtagtcta tggttaatta tatactcatt agtttcgctt ctgaccctta actcttcgtt
     9481 atgtcttttt agtgtgtttt cctgattgac agttagatgg tctacggctt taagatactc
     9541 gtttaatcta tctgcctcac catatttcag atacacccct cttactccta acttatgacc
     9601 tactagcatt tccctgatct cactatccat tttagagcgt cccatttgag taatacagta
     9661 cttgcgtagt ccatgatttg cggcaacttc atttcttcgt ttaccgtttc tgttggtttc
     9721 cgatatagat tgatactttg ctataccgct ctttatcagc agagctctga ctttaatctt
     9781 taacgatggg tatgccatcg gccttgcatc tttgacagta cttctatcat tttgattgaa
     9841 gtcatgtcga aagagcggtg agtcaggact tagaacttct ccaaatctct ttctatactc
     9901 caagtattct gtaacggcat cataacattc tggcgtacaa aacgtatagt actcatctct
     9961 ggtctttctg taaactacta ttttgaaaat atcataattt gacactttag tcaagtccct
    10021 aattcttaat gaagggatag caccaacacg aataccacta gaggccatta gcaaaataat
    10081 ggccttgtac tttagatcag caatgctaag aagtttagct atctcctcat aagtgtactc
    10141 tctatcttca tttgatttct caagtccgcc tacaaattta ctgatttgtg cccaaggcag
    10201 gacaatgtca ttcatttcgc aaaaatgctt aatcgcacac aatacaccat tacgactata
    10261 ataggataat tgtctatcct ttttcaaata tgtcagatat ctgattaagt ctgctttcag
    10321 gctggttggt tgtcgactta ccaggtcctc tatctgatag atatttttcc actttgcaaa
    10381 atcagctagc agtctagtat actcttgtct tgttctgtct gtatgcaatg attcgataaa
    10441 attctggtat gcgggaccct ctaagctaat tgtctttgtc ctagtcgcct ctgccgcctg
    10501 actagcctta gttaccgtgg tagacataca attactatat acggaccgtt atttatagga
    10561 ttgttgtagg ttgcataaat tctctccaat aaggattgta gcttgagtat cgaggatggt
    10621 ctgctcaata tacctaatat caacattatt ataatcataa caagggtggc gatctgttaa
    10681 tctttgtact aattgttcgg ttgattcatg tttaggcatc aatattctga tttttacgtt
    10741 tctttgttgt accgcttctt ctgctaactg tactgccccc atctttttaa tgacgaagaa
    10801 aggcattagt agtagggaat actatcataa tttccttttg tgcatttttt aaaatattta
    10861 gatagaattc ccttgcttta ggcgcattcg gaataacttc aatatcttct aaatcaacac
    10921 catcctcaat gcttgctatt ttgtcggtag catcgatcga gtttttccat aagtgttcaa
    10981 ataaagattg aaagtgtcta atgtatttag gttcattact agtaaaaatg ctatctccaa
    11041 tgtcagtacc ttctgtattt gctattgaac cgacaatttc tttatctgaa actccaaaac
    11101 tcatggggat attattgata tgtcttattt ttactccagc atctaagaat attttaatta
    11161 aatctagact ctttttgtct atagtagtga tccatcttat ccctcgatga tcgccttctt
    11221 tttccttatc tagcgcttcc ttaattttgt catagaaatt ctcgtaggag aattgtattc
    11281 tattagcttg tgtgcaaact gataactctt tagagctttt gatataattg atgaattcgt
    11341 ctttcttttt tgcatgttta tctattactc ttgtctcata atttatcaca cggcccaatg
    11401 cgttatggaa tatgagtgaa cgatgtaatt aaagttgtat tatatggaca tgacggatta
    11461 agctttgaac actattaact gacagattgc gccgccagct catatggaat tcctctacat
    11521 ctgtttatta agagattaag catgcttgca gctcctacag atgcgatgga acagtcgacg
    11581 taagtgaatt caatgtgctt ggcaatatag gatcgattag ttcgctcctg aaatctttgt
    11641 catgattacc gttggattcg aattgaggag gaagaaggaa gctatttctt gttcgcaaat
    11701 tcgtctttat ttcgttactg gtttactcct acaagtttta ctagtttggg taatattgag
    11761 ttgttaaaat aaaattgatt ctctaactaa ttcttcgctg taagtttatc tcctttgtat
    11821 actccattaa cacattaaca aacagttgct ttgcaaatga gtttttgctt aattgatcca
    11881 ttgatccaag agatgatatt aagtcctgca tttgcgattg cattgagtta aatcgttctt
    11941 ccatgacagt tagtttatct tctttctgtt tctagttctc gagtgtttca ctatatgtat
    12001 catacattaa tatcagtcta cacttagcac agagcttgct ttctggatca gttgttatct
    12061 ttaattaaaa caatgagaat gaattaacct tagaacaccg tacgccatcc atgtacttac
    12121 gtacattcat caaaatgtgt aggctaactc gtgacaaata tcaggcagca tcaattagct
    12181 atcggcaagt taaacgtaaa tgtagttcct tcgtcaccag cattgttctc agcccaaatc
    12241 tcgcccccgt gagcttctac gatactctta gaaataaata atcctaaccc tgtacctgaa
    12301 aaagattttg cagcaaattt tgaaaatagt ttgggcaata tttcaggatc tataccggta
    12361 ccagtatcct ttacacaaat aacaatcaga tccgtatctt catctaatgt tgtcgtaata
    12421 ttaatagtcc ctttctcagt aaatttaaca gcattactta gtaaattaga aataacttga
    12481 gttattcttc ctttgtctgc ttctaataaa atatcttggg gatggtataa taaccctaca
    12541 ttttcgccat taaagtctct gctcaaaata atatcatcga gaccatttag tattacatca
    12601 tttagattga atagttcttt cttcaatagt agtgacttac tttcaatttt tgtagcatct
    12661 aatatatcgt ctgtaagccg actcaatctt tttgcatttc gagaaatagt atccagtgat
    12721 tccttgtact gttgaatatt ccctgtttta gaaatgagga gttctgataa accaagtata
    12781 ggctgaatag gtgttcttaa ctcgtgtgct gctatattga taaactcctt ctgcattgta
    12841 tcatgatttt ttaattgttc atgtgctttt tttatgtctt catataattc agtttgtttc
    12901 cataaattct caaatattga aatataagac aaaactccag ctttgctatt agaatatgtt
    12961 gataacccta ttgcgtcatc gaaagttgtc tttaaatcat cttttatctc catcactaac
    13021 gatgcttttt tgtctattac tagaaatgtg gcttgagtac cagacgtttg ttcaatgtat
    13081 ctaacatcaa tattgctctg ctgttctctt aggttttgta ccacttgctc agttgatcta
    13141 tgtgtaggca ttaatattct aacttttatg tctagctctt taattgactc tttgcataat
    13201 tctattgctc ctattttttc ttgacgaatg aaagcattgg tagtcggaaa cataaacatt
    13261 atctcacttt cagcattctt tagaagattc aagtatatct ttcgtgctct atctgaattt
    13321 gggataactt cgatgttagt tcgctcagct ccttcttcaa tttctctaat tctcagttca
    13381 gctggtattg ccctattcca aaaagaatca aagacatatt gctgatgttc tacaagttcc
    13441 ttgacattac tgaaaataat tatagtggca gactgtgttt tatcatgtga agttgtagga
    13501 gcgagatatt ctctttcact tatcataaaa ttacctttta taccatccag atgacgaagt
    13561 tcatcaacta ttttcatcat ctctttgcaa gaagatagat tgtcatttgt aatttcagta
    13621 agatatcgta attgtacagc tttactttta gcctcaataa atgactttct tattggttca
    13681 atttcaaatg ctaatggtgg tcgcgtataa tccatgcaag tatcgattct gctctttgct
    13741 tcagagaaaa aaagcaattc ggcgcctatc acattctcag atccatacaa tacctctgtc
    13801 ttttctatga cgtatccttc ttcaatttct ctcatttttt gttctgccgg gattgcctta
    13861 ttccacagga tttcaaatac atattcctgc tgttgtatgt cctctttgac attgctgtaa
    13921 atagcatgcg gtatagtcgt taagttcgat tcaatcagag tcgcggtagt cgtagcaata
    13981 tactctgtgt cacttactcc aaaatttcct cttactccat ctaaatgtct gagctcagca
    14041 atcttcatcg cctgcttaca gcgatataag ttatcttttg taatttcagt aacaaatctt
    14101 aattttacgc ccctaccttt agcatcagat gcagccttca caatagaatc aattgcgaaa
    14161 ggaactattg atatcgattt aatatctgca aaataatccc atctattcgt tgcattataa
    14221 aagatttgta aatatcttct agttataatt tctgtgttat gtaaaacctc gatcctctca
    14281 gttttattga catctgcagt cgaaattgtc aaatatgatc tcaatattac cttatccatc
    14341 catttaaagg cacaggtttt gtacaagtcg aatgcggatg cactcatacg catcatggcg
    14401 aaataattct tttatagttc aggccacact ggcatattac gatagtggcc ccttgacact
    14461 tcggggcatt gtttgtccgt ttaccgctcc gattatggct atcctcgact tagggatcta
    14521 gtgcccggac ta
//
LOCUS       DAOR01000088           13746 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4149, whole genome shotgun sequence.
ACCESSION   DAOR01000088 DAOR01000000
VERSION     DAOR01000088.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13746)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4149"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         4146 a         2898 c         2783 g         3919 t
ORIGIN      
        1 cggttgtagc ggcgtacaat gctagcggca aaaccaccag tttagtcaat gataacagcg
       61 caatctgttt tgcatgaaca aaagccgaaa taagctttga agtatttaac tgggcctcag
      121 caggtaggca agttttttgt aagaccactt tcaacacgga aacgaagaaa acacccttac
      181 gacttggagg aagaagacaa ggagatccta gcaagccgat ttggatcttc aggtcatggg
      241 gtttcattag tggagagtcg agaatttaca tacgacactc agggaggctg gcatatctcc
      301 aaatatcggc tgactcaatt catgaattga tgagctttct agccggactc taacactaac
      361 gttaattttc ttatccataa tctccagttt ataggtaccg cttgtttttt aatctaaagt
      421 caatttgtta cagcacattg caagttgcaa tatatttagc tgcctcattt agattctgat
      481 gtatgtcata agcgtcccag gttgctcaat ggagagagat cgtgggtgga tattaggcta
      541 gcgtaaatca ttttcctgat tattgccgca ggtaccgcag aatttggcac ggatcggaag
      601 tatcgctctg cattctttgc aggtcttgga tggtgtaatt gtcgagggac taccgatctc
      661 catttttctg gaaagcaacg cctcattgtt ctgagattcc ggcacagaat actcttgccc
      721 tacggtgccg acagaagata gaaaactgcc aatcccgaag ccggcagttt gggacgccga
      781 agacgaagat gcggctcccc cactagcatc ttttatactt gtgccctttt ccaacgcaga
      841 cctcagttcc aaaattaccc tattcataag gaaagtcaga gctgagatcg tgaccacgta
      901 attgagaatt ttagaaaaga aatccttttc attgatcttt tcctctttgt atagattcgt
      961 tatatcgata aatgaacggt gatgatcctg agtttcgttc attagctgtt ttagcttttc
     1021 gctcagggtt ccgaggacat ctacaccaaa aggcatcata gaaaatgtta tgcaaagcga
     1081 ctattaaaat tagaacatct tgttattctt ttattagtaa ctcaactgta caacctatta
     1141 gtaaatagta gcaatttttt tacgtcttcg caactcctct cttgtctttc cgcttttttt
     1201 gccactctaa tcgtcttgaa ttaagtgatt caaaattttc gtagatatga ggtctgtggt
     1261 ttcgcatgcc ctccacgatc actctgcact tctcccaaaa tggggcaggg ttgaaaacgt
     1321 cgaaataaag cttttgatct attaatctag aatttacgag aacccctgac aactcaaaaa
     1381 agccacagac cgttgtgaaa tgacacctcg aacttgaggc ccctgcatac ttttgatcat
     1441 attcttcgta gctaccggca tctagatcct ccagaaacca aagcacggaa tctctgtgcc
     1501 gatcatatat ttcgtacatc ttaagcaatg tttcgacatt tttagtgggg ctcaatcaag
     1561 agatcattct aacgctagta ataaaatctt ctgcctgatt ggtagtttgt gtaatacaga
     1621 cggctaagga gtaccaaatt catcctgtaa taaggagttc gttgatatgc agatggtgtg
     1681 gacacaacaa ttcgactgga catgggaata tggggtgaaa gagagagaca taagaatttg
     1741 aagtaaatat taaatagaat aaatgtatat gtaactgtga catggaatca ggcattcagc
     1801 aattagaaat agctcctggc ctgaaagagt cgttgctaag agctgggtta actatagagt
     1861 ctattgtgtt ggagggtcca ggcgctgttt cggctgcatt aggaatcgag ccatacgtcg
     1921 caaagataat ttatgatgcg gcaaaaaaaa tagcaacgga aagttctatg gtggcatcat
     1981 agcaggctca ctcactatga taatccaaaa aaaagtaaat tagtatggct catgtaagcc
     2041 caagcaactg ccgtcgcatt cccagtccca gtacgttcac catgctccgt tcatttcttt
     2101 gaataaaaat aaccttctcc atgatattgt ttatcttatt tctgtctccg aggacgcgta
     2161 ttactcttaa atctaccgcc cctatatcgt agactggaaa tgctaaatag tcacttgtta
     2221 ttttaccata ttgtacctat ctcattacca gtacttgaga taataaagtc cagataggtc
     2281 tggttcatcg accattgata tgggttcaaa aagaaagaat tgataaaacc gcttttccga
     2341 tgaatgcatc tataaattag aacatagcaa ctgcgaccag gataaaacag tttggatcca
     2401 ttttttatca tatgttacat catagcatgc aattgatagg aaaatcctgg atagtcttca
     2461 ttcaggcgat aatactctca attgaatcgg tgatcgtgga atttctaacc gaaatccctg
     2521 gtatatcttc tctcctggtg gcaggcataa gcatccccat cgcgggcgca atcctgctaa
     2581 tggcgcgaac tgttttttta aacaaggaac tgaccgtatt caaatcatgg aaattattgc
     2641 ttagtggctc gatattcttg gcagcggcag tatttttgtg gtacgattct gtgaccaaag
     2701 tcggagcatc aaaagagggt cttttagctg ggccactaga gactgtagtt gtattaattc
     2761 ttgcatggtt cttcctaaag gaaaagttgc gaaaaatgca gcttataggt gttaccatcg
     2821 ccctatcagg attcttagct actgtcttaa gtagttcttt acagctgaat ttgcaattat
     2881 cgacttcttt gacattcggt gacttcgaag caataatttc agctatcgct ttcgctgcag
     2941 gagtaatatt catgaccaag ttggttgaaa gacacagttc cagtgaagta tctggtgctt
     3001 cgcttttgat ctctggccta atcttgaccg cgattctcat tctatgtccg tcttcaacat
     3061 tacatcccgg agttaaggat tggatatttt tggccttatt ctctctggta ccgctggctg
     3121 gtgcactgct atatgtggta gggctcaaca ggataggagc ttcgctaacc tccaccatag
     3181 catcttctaa tatcctgttt accctgttct tccaacttct cttccgtgga attggaatta
     3241 gaaccaacat cccagaaaat attctgctag cggtcgtagg aggtgctttg ggagttttcg
     3301 gaatctattt gattcatgta gaaaatgatc ttttatttcg taagatttag cactttacgc
     3361 tatgtattag tatagaggaa tccgactgaa atacagaaat atattttaga cttatatcta
     3421 catctttgtg caataagcct acgtaaatcg gatagtgcta gtttgttttg gaatgatcag
     3481 ccgaatctgt ggttttcatc cacgataccg tttttgctga tctaatgtcc gactgtcatc
     3541 ttatttttgc cttgcacaaa aaataggaat tcgaataggt tataatgaat cctatctgtc
     3601 aatccggcta gtatgccaaa gtccattgtt cagagctaac cgttttgcaa acgaggctta
     3661 ttagtcgctg aaagtacttc gagtgttaca gaacaaaaag gtaaaaaagc tcctgccagc
     3721 caaaatctaa aatgcagtag atctggcaat ttcgtccaaa aaatgttcaa agatagtctc
     3781 accttcgaat gaataggatt atcaggtgca aactcttgaa agtgtcgcga gtgttctgtt
     3841 ataagactcg atcgagttcc atcgagtaca tcaacgtcgt ccaagccgaa gactaagtgg
     3901 cttgaatagg aagtgaaaaa accaatagct tttgaagcaa aaaaagttat gtctgcattg
     3961 tcataattgt tgtttacagg attataataa aacaatttac tactgccaag aatcatttta
     4021 cttttatcgt ttgttacctt tactttcatc aatatcatga gacatggact agtcaaaatt
     4081 aaaccgtata tgcttatacc gcttttcgtt gtcgggacag tcgctacagc tgttcttctt
     4141 gtagataatg ccaacttgga taatacggcg tatggccaat cactattatt tccaaacgct
     4201 cagaattcta gtgcttctcc caatccttca ccaatattac aacattatca gcaacaacgg
     4261 ccaccactac ccaaccaact atatcaatct aacctacact tcgtcaagat taccttgcct
     4321 actaaaggcc agcaggttct agttggtaaa gacctattaa tctatggaac ctctgcaggt
     4381 aatacaactt ccggatgcaa ggtgtcagtt aaggcaaata atatcagtcc atatcatgac
     4441 gcctcaccgg gcggacataa tgactattcg aaatggaact ttactctttc accagcgtac
     4501 acggctatta aacaaggtca gaacaaaata actgctaaat tcgcatgtag caacgaacct
     4561 accttggctt cacattatag tgtaaatgta actggtgtag gtacaggtac tactccgatt
     4621 ggcccaagcc atcagtttct acaaccaaca ttgagcacaa atggaggagt taattcggcg
     4681 gccgcgaata cgactgctac tagcactact aatgctattc atgcttttct gtctaaacat
     4741 tcgcctgtaa acaataataa ccaccgagtg tcattaatat cactctcaat tcggataggg
     4801 aagagttctg ttcatccagg agatacagaa attgttacta ttatggctgt tgataaaaat
     4861 tcgactaaag caattactgg gggttcggtt ttgggaaaca ttagcagttc atcaggattg
     4921 cttaagaaat tcaaaggcgc tacggataac aacggcaagg cttcatactc ttggaaagtt
     4981 agtagcagag atataacagg taagtataaa gtgatcgcgc aaatatattc tccagcttat
     5041 gagagcaaaa cagcttcaaa aacgtttaaa gtaacttccc tacctgttat ggctaccagt
     5101 ccgagtagta ctaactataa tagcctcacg tcatctaacg atgctcgtaa taacaataac
     5161 agtaatactt caaacgcagc tcataataat aattccataa acaagaatcg tcatcaacat
     5221 actttgccta tagatacgat tagtagtggc aatagcaata gtagccttac gccatcctca
     5281 catgctcatg gtactaataa caatactaag aaaagcaacc ttgctacttc aaacgcatcc
     5341 catacagctc atagtaataa ttctaaaaac caaaatattc accatccaac tccaactata
     5401 actcctgacc atccattcgg tgttccaata agtcatgttc cgtttgccat accatgaaaa
     5461 tggccctatg aagcgctccc tgtatcgggt atcaattatg gtgaatggtt cctagttact
     5521 ttccccatct acgactatgg tgaagacaag atgttcgatc agcaactggg agtatgcagt
     5581 caatacgctt catcacaaga cgagacagct gtatccctag cgaaccatcc agctaattaa
     5641 attggcggaa atatcttgtc tttcatatcg aggaatccct tcctttatac taggccgatt
     5701 gctgccccaa cgtcagcgac ttccttcctg cctttaattt caatgacaat caaaaagcca
     5761 gtaggttctc agcgatgatt ttgtagtata tattagcata gcctaatcta gaacaaccac
     5821 tacaaaaatg cttactattt gatagacaca cgcgaattat cgaagatgtt atttaccttc
     5881 atctccttcc attaggtaac tagcaaaatc agtcgaagta ataatgccta acggcttgtt
     5941 tacgtcttca tcctcaacta cgagcaagtg gcgcacttta ttctgaagca ttaaatcagc
     6001 ggctgcttca acagaagaat ttgcatcgat tgtgacaatc ggagaggcca tgatatcttt
     6061 tatgagaact gttttactgt ttgcatcgtt agcaaccact cttcttacca gatcgcgttc
     6121 cgttattatg cctattggct tgtcagttag gtcagtgact accaacgaac tggtattcac
     6181 atcacccatt ttgtgtgccg cttcctgagc tgtcctattc actataatct tttcgagctt
     6241 ttcattcatc agcctatgaa ctggcgttga agtagtcatt cgatgaatct acaagtgcat
     6301 tctgagatat aagttgcgag gctttcacga tagatcaatt gattttgatt acgaatacta
     6361 catgtagtca tcaactaaac ctgcccatac aaagaactaa ccctcactca caattctata
     6421 gcaagacaga caacggagag gaggtgttag cgatcagcta ggaggggtta aacctgtagc
     6481 tcgcaaccgt tgtttcattg tgcagggttc atcactttat cgcatttacc catgattaac
     6541 aatctttttt attccggata agcaaactac gtacatggaa tttacagaca ttgagacacc
     6601 acatatcaaa aagccgatac tcgttgccga gatgcaagat atgggcgata tcggcagcgt
     6661 tgctatacat tttataaata agaatctcaa aacattgcca tttagatatg tctctgcttg
     6721 ttatccgaat tatgtaatgg accgaggcgg ttacatcgat ttccaacaag aacgatggca
     6781 gtaccgatac gcacaagacg taatagtttt tgggggaggt ctggggcaac cgcaaacaaa
     6841 cgaggagtta tacaaactct gccaagatgt gatcgatatc gccaaaaaat attctgttca
     6901 actgatttac acactaggtg catttcatac tgaaaggaat ttacagaaaa aaccgaagac
     6961 gttcgtcact acgacatctc aggcattgac agagcaaatt aaaaatttag gaatcgaacc
     7021 tactccgtca tcatcactta tcacaggttt caacggctta attttgggtt ttgcaaaact
     7081 gaatgacatg caaggaatag gattgtacgc tgaaataaac gatccgcaga taccgcagta
     7141 tcgttcagca aaaagtgttt tgcaattact agaaaggttg acctaccaga agttcaagga
     7201 tcttggagaa ctcgatgcta tgggagaggc tgtagataac gaaatagaca ggatgaaaaa
     7261 gtccggcgat atctgaaatg ctgactgggc gtggcttgcg tgaataagcg gaacttcggt
     7321 tgagattgtc atcgcctttc taatgtttgg aactcgatga atcttagagg tatgatcaat
     7381 tctgcgatca tctatagttt ttaaacgtgt ctacaatcca atctacgtcc tgctcactct
     7441 ttgcgagccg gcctatcttc aggcaatccc taatatcctt tgatttcatt ttccaccaca
     7501 cggcgtttgc tatcttagag gctagttcac tcccgacacc ttcgtgcaca agcatgtggg
     7561 atgccacttc atgaaagttg tcaaatttat actgtttaaa atgcaaggta acaaatctcg
     7621 ataataacgg tgttagcatt cgcttggtgc cattgctcgt agcaaacacc catgttttga
     7681 gttgggtgtt tcgtgttttc tggaatttcg tttcagaaag tatgccagtt tccatcaagc
     7741 tcaagagagc tgtctggtcc tttatcccca tatgttcaat ttcgtctacg accaaatacc
     7801 tgggtcgttt ctcgaataaa tattctagca taccagacct tgtactgtga ctgccaagag
     7861 tgaagtagga ccgctctagc ttcatgcact ctagcatgaa gagtgtctta gccgacgcgg
     7921 gcggaccaac tagtaatata tgaaccggct tctgagaagt gaatgacagg ccaaagattt
     7981 tcttaacatc accataccca acaatggtag aaaaaagttc gccagacttt ggacacgaat
     8041 ctttggaggg aagatctctt gtattcactt gcattggaac cggtaactca ttttctgtgg
     8101 acacgactag tttaggttta gttaggattg atatacatat tgttgagaaa ttgtgttgcc
     8161 gaattgctct acgaatctag acatacatct attgagattc aaaatacttt actctattat
     8221 caagtctcat tattcgacct aaacaaatat gtttttgttt gtagaagtcg tattaacttg
     8281 aatacttcca gcaagaagaa caaaagcagt aaggccgcta ctagagcaaa ggaccattca
     8341 atccagcggg agcacccata caggacaacc aaacaggcga gcaagaagat aagatatttg
     8401 caattgtcta ccaatgtaaa cgattatgac acaggagaaa cagagacagc gactaaaaag
     8461 accgaatctt ccattgtcac atcgaagaaa accggagaca aattccaaga cctcgagaaa
     8521 taataaatgg ggcagctgtc ggttaggatg agcgtggagt tttgccaaat cggatttggc
     8581 gaaaacgaat ctaagcaagg aagcggaaat agacagggaa gaatatccgt aggaagtgta
     8641 catcaagttg ttgtgtacat tcgtaaaagt aacggtcatc ttaactggtt gtgtaaacca
     8701 ttcttgtttt tttgtatctt cgtacgcatt acaaacaaga ggacacacgt taacacctat
     8761 cgatttaatc tttgaataga atatattatg ttttaaaaaa atgtgaatgc tgaacgctgc
     8821 tgagcttgcc ggggtagctc agcttggtca gagcgttcga ctcataatcg aaaggtcgcg
     8881 ggctcgagtc ccgcctccgg cactcctgag aaaatcgaca actctgtatc gtcatcacgt
     8941 acaagactca aataatttga ttgattaagt taatcatcct agccgatgaa gaactatcga
     9001 aatccagtgc ctacagtcga tgttatcctc cagcaagaat ccaatatctt attgattaag
     9061 aggaaaaatg atccattcaa ggatcatctg tcgttaccgg ggggttttgt aaatgaagga
     9121 gaactcgtcg aggatgcggc caaacgtgaa acgtgggagg agacatcgtt aactatagag
     9181 cccatagaaa tacttggggt ttattcagat ccgaataggg atccaagggg acatgtgctg
     9241 actgtagtat gggtagcaat agtcgtcaat ggcgttgcca gggcgaacga cgactccatt
     9301 gaagttcaat gggtgtcgct aaatgatata gactccacgc gactagcatt tgatcacaag
     9361 cagatactcc tggactatat ccagtggaga aaggctggta gagaaacctt ctggtcatcc
     9421 aaaagaaggt tcccttagtt ttatcttatc gagatagcaa aaggcattat gatcccaaaa
     9481 attttattgc agagctaaca tttttcgcct gattggagcc gtctaaatcc ttttacgcag
     9541 attttcctgt catagagacg attgaaataa aagaaatttc agatatccac gaggccatca
     9601 aaaaaatggt gcaaacttac gccgttagga atgatgccct cgaattcagc tatcgactct
     9661 tgcttcccag gggtgaagag ttgactacac agtcgaagag aatcggcatc accgcccgag
     9721 cggagttcct attgtcacta agaattaaaa agctgaaacc aaatcttagg gaaattcgtt
     9781 acgtgcacga tgtggatcac tatggctggc ttctcgttga cccgactgtc tatgcccgat
     9841 tctgtgctgc gaggcgttag cagtccgacg accattgagt acaattaaag gcgaactttg
     9901 aagcactata ggggccttcg gagggatttg aaccctcctc gagcggtatc cgtcgaagac
     9961 aagtcgtcca cagccgccta tactaaccag gctatactac gaaggccaac agctgctcct
    10021 ttcgaaatca ctgtatataa ttgttagagg atttgtctat tttctataag ggctttctaa
    10081 tatccaattc taaatatctc tattttaaat gagacgcgga accaatccga gtccgagagc
    10141 atattctcac aacgttatcc caggatgtct cacatttata atttcttggt tttgcacgca
    10201 atcttgtatg acatttacaa tatagagaat cagttttgaa atattttcga tcaatatttt
    10261 acaccacctc tataaatcgt ctggcctttt atgattggcc cgcgcgccgg actcaccatc
    10321 tctggcattt tgtgacaagc aaaaacgcgt ttcgtggact taaaaagtgt tcgcgcgaat
    10381 gtatcgcgga tccgcataga atgagtgtat cgctgctact agatgagcgg ttgcgggctt
    10441 gatacattaa tgtatgtatc atcgatgtat ccgcgaaaag tgggcgaaaa atgacgcgaa
    10501 cagatttctg tccgcggatt ttgttcacaa tgtttacggt cttgtagttg tacccgtgtt
    10561 tttgtaaaca ttctgtttac atgcatatag atagatgcaa gccagtattg ctttaaattg
    10621 agaagaaatc ttatagaaga tattatggaa gacgaatcca agacggtttc acttctaacg
    10681 ttaccatgaa cgagttttat cctagctgat gcgtttctga attcaggaat ctcttcaact
    10741 gaacggatca tatcagaaac gtatctggtt attccagaat ttagcttgtt gagaatttca
    10801 gttcgcatat attcatcgca gggttgatga atgtttacaa gtggcatctt caacattttc
    10861 gcagtgtcca caacatcgtt gtaaatgttg gcatgagctc ttaaatcgaa tttttttttc
    10921 agctcttgta cggctaacca ggcctcacgt tttggtacgc ccatttggac cacgaaatcg
    10981 acatgtctgc taaagacttt agaaaatctc agagcagaaa ttcctattgg atgatgtgca
    11041 ataactgcgt cgcatccgag actcctggcc aacataagct ttgctgcaga gacgtaactg
    11101 caatcagcac ttttttgata tttccaccag ttacgtgaat gatgttatcg gaagggattt
    11161 tttccagttt acacgatcaa ggttgatttt cattatttct tgagtactaa ctgtcgaggt
    11221 tctcagtgct cctctaaccg tcttccaaca gcctacaaaa atagagtacg aagcttatat
    11281 ttcggataac cagctaacaa gatagtcgtg atgctcatgg aagctttaaa ttcaattcct
    11341 tttgaagaaa attagatgag ctgctcggac gaacacgttt cacatgttac gcaagataat
    11401 ctcatacaaa tcagtcccgc tgtgaaatcg aggctgcaaa aggcaaaata cggcgtatac
    11461 aaccattcgg ccgtcgagtt gtgtcattgg acgaaaaaat ctttttcaaa tgcaggtaac
    11521 tgttataagc acaaattcta cggcatctcg actcacaggt gcatggaaat gactccaact
    11581 gccatgaatt gtgagaatcg atgcgtgtat tgttggcgtc cgactgaatt ctacgataca
    11641 cttcaaatgc cgccagagtt ggttgacgag cccgacgtta ttgttgaaaa tttgctggct
    11701 gaacggaaaa aattgatcaa tgggttttac ggtcatgaaa aaaatgacaa gaaaaagctc
    11761 gacgagtcac tttttcctgc acattatgca atatcgttgt ccggcgaacc tacaatgtat
    11821 cccaaactac ctcaattgat taaatacctt ggaagattgc ctgcaactaa atcgattttt
    11881 ctcgtaacca atggtcagga gccggaaatg cttcagaggc tggcagatga ggattcgctc
    11941 cctactcaga tatatttgtc cacgaatgct tcgaacaaga aaatgtttta cctcgtgaac
    12001 cgtccccgtc atcgagacgc ttgggaaaga tggcggaaaa gtctaagatt tctctcaact
    12061 gcaaatacaa gaacagtact caggatgaca attatcagac aatataatga cgctatgaat
    12121 tttgttgacg aattcgccga aatgatgcaa cagggtaacc cacactttat cgaattgaaa
    12181 tcctatatgc atataggaat gtctacgcag cgacttgagg cggagaacat gcttgaaatg
    12241 aaggagatca ggtctttttc cgagcagatg tgttttaagc tgcaagacta ctcaatcatg
    12301 gacgagagtg aaatatcgag aatcgtggta ttacaaaaca tgagacgata cacggacaga
    12361 tggatcaagg aatacccaat tgaagcatag aggggtccac ttttcttgat gcggagttaa
    12421 ctagctagac gacatagttt tgtagaggtc ccacatcgtt tcaaaaaaaa taacgaggtt
    12481 tatgatatgg ctacaaagta acggtcaaag gatgcgagga tcggaacagg gtatacggtc
    12541 aattacaccg acaggagaag ccgggatcga tttcagaggc actctttgaa acgcctgatc
    12601 tatacacagt ggtcaacgaa aggaggacca taaaacacgc cctccgttgc ccatatcgct
    12661 gctgacaggg cagggcatat gacttttaag agttcatatc tataccttga ttcggcaaat
    12721 atggttgtaa atggctgaat ggggaaggtt ctctgctcaa gatgcccatc tttgagtttg
    12781 tctctagtat acgaggcggg ttcaataccg gcgtcgtcct gtttagactt agggggctac
    12841 aaaactatgc tttaactgct ccacgctcaa ataatttaca ctaggccaaa atttttcaaa
    12901 cccatatttt tatagtccga tctctagaga cgtttgcata attgaatctg gaaaaattac
    12961 tgttgtcgct ctcgttttct ataatgcttt tattcaccat ttatagcaat tcaacttatg
    13021 ctcaattagc aaaaactata gctattaaaa tcacttcgcc agctaaggga caacaaattc
    13081 cagttggcat caaaaatttg tcaatctcgg gcacatccgc atataattca acaatgagtt
    13141 gtcaggtatc gattattgtt gatgatgtga agccatacca aaaggcagtg gcggcccatg
    13201 ggggtgatta ttcctcgtgg aattacaccc ttacacctca ctatactagt atcaaacaag
    13261 gcccaaacaa actcactgct aaactatctt gtcatgataa ttccatgaat gccacaaaat
    13321 tctatagcgt aaacataaca ggagtgacat cttcgtctaa acaagcgggt gttaatacat
    13381 cgaaatccac tgttgcgaac caaaccaaaa atgaaacagg aaaagcaact cctctgccgg
    13441 ctaaacaaaa tgtcacgaca gcattcaagg cattacagcc tacggagcaa ttaccgagca
    13501 cacttactgt caacggtata ctggcgaaga caggcatatt ggctgggacc cagcagcagg
    13561 gagcgacaca atccgataca aattcgacga cggcgcaaca tcatcgtcat cattccccag
    13621 cgacaactgg aaaaactggt atccttgcca ccgtttctgt tgaagcgcat actccaaatc
    13681 gccatctaga gaaaacagcc acaccaacga cctctaacaa tacagcttct aacaatacag
    13741 cttcta
//
LOCUS       DAOR01000089            4296 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_64712, whole genome shotgun sequence.
ACCESSION   DAOR01000089 DAOR01000000
VERSION     DAOR01000089.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_64712"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2431..2559
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1184 a          789 c          730 g         1464 t
ORIGIN      
        1 aaagatacag gagaagttgt cccaaatgca ggtgatcttt caggaatttt atacatttca
       61 aagcctccca gagatttact atgcctccaa atcgcatttt gtttgtcgtc ggtaaaccaa
      121 atactatctc catttcggtc aaccttaaca gaccatacgt ccgagaaatc catgggattt
      181 tggcgcacct tccacatagg gatgcctatc tcattatcaa attgttttgt aattagatta
      241 taactgccaa gtgcgccttg cttcgtagat acataccata cttttccagc ttgactatca
      301 actgctattc ctaaaggcat ttcacaagta tgtggaagcc tgtattcggt tacatagttg
      361 tttgagttta gatttgaatc aagcccacaa aattgattct taaattgctc gatggaagaa
      421 gcgtttgaat ttgcaacaat tcgtacaaca ggctggataa cttgcttact gttgctagga
      481 gatctaaata gcggattagt atacatcgcg acaattatta ctattattgt aattgcaatg
      541 attccgtaaa ccataatgct tttggaatgc atcatagtcg acctctacct aacgttaatg
      601 ccatatcatc tatgacttgg attttatttc ttctttctat tgcagatacg acttgcaatc
      661 tattcatgta aatgttttct ttccagagaa aaacaatgtt actaatatag acaataactg
      721 acgaaagtat ggtaaatgaa attgttgttg caaaaaaatt gaggaagata ttcgtcatgt
      781 ctatgatatc catgtattca taacgaatat cagttggtgt tgttgttgtc gttgcaattg
      841 tcatattcat actgatttag gtagcaccaa tatttgacca accatgtttg gcagatgtat
      901 gctacagaag aactttatgc ttcctggatt gtttgctatg aatgatacag tgtggatttg
      961 tcctctcaga agagcaaagg ttcctactcc atcgaccgtt atgagatgat gtacgccttg
     1021 tacgcctatg aaatggagtg tgaccttgtc gccttggtta acgactattt gatctggagc
     1081 aaacgtaaat gatctgaagt cccaggctcc tacttgattg ggtagggtta tcctgaatcc
     1141 tccgccagtt ggtatagtcg agtttacagg aggcttctct gctggatgca gtggagtatt
     1201 ctgtgcgcaa ttaccacatg ctacggcagc atgtaagctt gttattccgt caaggtgcac
     1261 tgtatctatc caaaattcct tgtttgtcga ttggcttgga ctaactgtta cagtagtgtt
     1321 tgctgctgat gctgatgttg tcgtcccgtt actgccatta ctttgtgcga gtgcgtgctg
     1381 cgacatttgg gtttgtattc caagaatcat agcagcagat gcaattactg ccataatagc
     1441 tgccgctgct atgattgctg aaggtttctt tattgttttt atttttgttg tcgttcaatt
     1501 tttttcgtta gttcattgta accttctttt gattataagg atggagacaa attccaagtg
     1561 cggattccat ctctggattc caaaactgga attcagctgc acgtttttat agttggtcgg
     1621 tagtatgaga tgcctcatta gaaattacca tttccagaat aatatttcct gtgtataacg
     1681 aagataatgt ctgattaatc acacaatgta cacaaacgcc agagaggcat ataaaattca
     1741 atgatgcatt aaagaaacta atcatccatc cggttgacaa gccttctatt atcttatggt
     1801 ttgtattctc attaagtgca ataatctgaa aaccctcacc aactgactat gttcgaattg
     1861 attaacttta gactgcttaa ttcaaaggat cctggagatt tttatgctga gcttaacaat
     1921 cagaatgggt acacaagtag tctaaaaatc atttacattc aacgaacgtc attgcaaaat
     1981 tcgatattaa agtgtgaact aaattgttta tagaaacatg gcagacaaaa caggttcgat
     2041 atgctgttag ttgttaacaa tcatcgatgg atagatctga acgtggtcta agcgattact
     2101 ttgatgcaaa gaaacaaaac caaactttcg ggaatatcgg tcctactcaa catattgaag
     2161 aaatgagtgc attttcacca acaccgtttt ccttagcatc tttatctaga gcatcatcta
     2221 aatccttgtc tatacgaata gttcttagta tcgtcttttt cctttccttt ctttctgtgc
     2281 tcaatagcca ataaccttca atcctgaata taacatttta atttttgaga ttttatctaa
     2341 tgttaaccat ttctgtgcat atgactttca attctggatc gaacatgatg tttcagagtt
     2401 gcttaagttg ccaaattcgt tgatctaaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atctaaatga cgtgttgatg
     2581 gaaattcacc attggccgaa caaacaaggc acaagatcag aatcacactg gccaatctca
     2641 tggagagatt gttgttcaga cgaatatcaa aaaaacttca gttttgaatc ttggatactt
     2701 aattcttttt cgcaatgctt tcttgacaga acacactcta tgagtattca tatatgtagg
     2761 ttgttggtat tgtaggtgcg gtattgtaga tgcgtgagca aattgttaga aaaaggcatc
     2821 ataaatcatt ttatctagag tgcttatctt aaattgttga tcttattcgg atactcaatg
     2881 atcctgtcgt caagcatttt atattgattt tcgtttatgt ctcctctgca gcagatagat
     2941 tatgtcacta cgtaaagtat ccaagaagtc cagatattcc tttgtgttta caggttttgt
     3001 ttttctgcaa agttcatcat atttaccgtg gatttttaat aaatatatac tcaagcattt
     3061 tgattggttt cttgtcctta ataatcctgg tacggttact atcgctccag tagcggctgc
     3121 tccggttcct acagctgttg agacagtaga taaggaaagt ttatgagtat tactattgtg
     3181 attttcctgt tgttgagctg agactcggat ctggtcaaag ggagagaagg ccgctgctcc
     3241 gataattaat agggatgcta ttgccatgac gaatattatc ttattcgttt tgctgccaag
     3301 tagtctgttt atattcaatc ccttaataaa caccttctct tctctaagcc aataatctct
     3361 ttcaataaca cgttaacatt tgctatatct gcgaaacttc attccttcaa caggctccga
     3421 cattgttgtt tgtagttctt tatttactct ttgtatgaat tcctttattt ctaccggttt
     3481 tctaataaag tgtactagct ctttttctcc ttctccactg tctttatccc cataatcgtt
     3541 tatgtcaagt cggtcgattg gaagtaattc tcttaatgct tcataattga cttcagatgc
     3601 tgtcacaaaa accactttaa ttctatcatc tatttttttg atttgttcat aaagttcaat
     3661 gccatccatc catggcatag caacatctgt aattaataga ctatagacac gagatttaaa
     3721 attctgtaat gctaaaagag gctcgttata aacatcgact tctttacatc cattttcttc
     3781 taaaaccttt ctgagagtaa atgcgatatc aggttcatca tccacaatta gaatcttttt
     3841 cttccttctc ttttcctgct tttcctgctt ttcctttttt tgcaactgac tcttcagtgc
     3901 taccatcacc taaaacaaca gaatcattgc acctacgagt agggttaaga tgctgctgtt
     3961 gcggggtaag tgctttcttc attacatcat tatgtttttg ttctgattat aaaactagag
     4021 acgaattcca cctctggatt ccaaatctgg aattgagcat cccattttta tatctcgtta
     4081 ttcttaggag aattagtgtc tataaaaatg gagcagcata ttaaatgatt tcaatccaaa
     4141 ctcaaatctc gattatccaa gctattgtct attttattgc tgctgcagtt cctatctatc
     4201 tctcttttat aataaagaaa tataataata gaaataatgc cttccgaaat ctaacaatgg
     4261 tacttgcagc ctttgtaata atacaaggta tatacc
//
LOCUS       DAOR01000090            3596 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_89229, whole genome shotgun sequence.
ACCESSION   DAOR01000090 DAOR01000000
VERSION     DAOR01000090.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_89229"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          949 a          779 c          524 g         1344 t
ORIGIN      
        1 attctaaact ttcaatagtc atcatctttt tcttattcaa agtcattgcc acgttctatt
       61 tctatacaaa tctggtagcg atgaactatt atttttcctt tagtaatcta gtttttagtt
      121 catagaaagg aagtgcagtt tcatataaag cagttagttc attgtaagag ataaacccga
      181 atctatcctt catccctcta aatggatatt tctgcatctc aatgttgaat ttacctcttc
      241 tatcctcagt ggcaacgatg aagaatttag agtacagatg ttgcagttga gtaagcctgt
      301 tcaaaccatg aacaatatca gtcgtgtgct caacttcaaa acataatttc ggattctcgt
      361 catcaccaaa ccagattaca tctatctccc tagcagatat tagatctctt tcacccgcaa
      421 atgcaggaat ctgctttaac aaagcaacat ccccaagctt ttgtccatta aatgtctttg
      481 attgatcgac agtgtaagtg agatatccca atattttacc caattctaaa atataatact
      541 cggcagcatc atgtgattct ataatagttc tagctaactt ttttattact tcctctttgg
      601 attcccctaa tttcggttct ctaggaatta tatcttcaaa aacatgccag aacatcatat
      661 ctagatcaat aaagtctttg attccaaatt tcgtcaactc atccttcaaa acgcttagag
      721 cttgaacaca ttgataatat tcctccccac tattaatctg atttttgaaa aatctgtctg
      781 gcaatatgtt taattggaga aaaggaagaa cagttttagg tttctcattc cacaaacaaa
      841 acttatctgg atataaaaaa tgcaaaatct cactaatcga tgaagcgcca aatcccgtga
      901 tattcttcct aaagttattg tatttccaaa ccatatcttc agagctgtaa agaagcttct
      961 caagttcgtt ttttattttc tccaatccgt taggaaagat tagtttattc tttatgtacc
     1021 agtctttatt tctccacata ttacttgccc acaatttctt ataaagttcc gtgaactcgt
     1081 tatctgtcat tttggcaaga ttttcggatg ataatctatg tttgaagtat ttttcatgtt
     1141 ctctgtgctc tgtgaccgta ttccttccct tctcagtgtt tagccagctg ttaaacttct
     1201 cagcctgttt gttcaaagtt tccttttgtt ctggtgtaag cgtgcatgat gacatagatg
     1261 gacagaaccc tttgtagctt agtctacatt ataaaattat cataacaact tttgattgtt
     1321 cattgtcagt ttgtcgctgc tgtagcggta ttttcccacg gtaaggaaga gatcgcttct
     1381 ttttttgcct caatgaatgt gaatacgtct ctttcctctt ctttcagtat gctaaacccg
     1441 tgggtcctca acacctgtcg aagatcggat aaccttcctt cagtaagtgc attagttgtt
     1501 tctgcaatta gtagaacccc ttcattccaa agacacctac gagcttctga aatataatca
     1561 atccaatttt tgcccattag cgctaatgag aagactatta catccaagtt accatctggc
     1621 aatggaactg atctcatatc acatgcagta actttttcat ttatagcaac gtggtcacag
     1681 ctataaactc tatcagatcc gaggacctcc atgattttcg cctcaccaca accgaagtct
     1741 ccaaccttga agcgaggaga tatctgctca atcctttcta tcattctctc gtaagggatg
     1801 attttccatc ctttccgtgc ttcacgatac agtctatgat actctatcca ttcttctgga
     1861 tttctctgca tcctgaaatg ggtggtctgc gaattttcag aattgagctg ctggtttagt
     1921 tttgaaaaat cactataatg cgatatcatt cctggtcgtt ctagttggct tttggtatct
     1981 gatctattgc tagcatcaat tacagcaatc ccgacaagtt cgattgctgg agtgacatca
     2041 aagtcccttt tcgtttcttc tagttttgtt ctatattcct tttcgagttt tgttctctta
     2101 gttttctctg tttcaatgag tctagaatag tttgggctct ctgcaagttt ctcctcatac
     2161 tcatggattt ttgtgtttat gccttctatt tgtgttgaat agtgccttgc aatagactct
     2221 tctgtcttgt attctatttc ggctagtcgc ggcgcggttt cctttagcaa atcatttgcg
     2281 tatttgtctg cgtaaacatc aaccaactct ttattttttg taatatctga tttatcatag
     2341 gatagtactg aatttgatga tggctcataa tccagtatct ctctcatatc gatctctttc
     2401 acgttgccat tatcatcact atcaaagtaa aaagcaaaca tcttcccatt tctttctcta
     2461 ccttttccat ctcttaccgc aattttgtaa atcatcaata tacccttatt gttaccatgt
     2521 ttcagaattg caacactagg tttggctgcc tgatgtacta agttcattat atggggattg
     2581 ccaagtgcaa agtaagtgta acctttctta attgcaatat cgctgctaaa gctaccttct
     2641 aatacacgtg tcgatcctcc tactggaatt gagatttgtt ttggcaaaaa tattctataa
     2701 acatcctctt ccatgttaat aggttcaagt ttgcctccct tagaatcaag atatttctta
     2761 acaaaccttt tcacttcatc cttggtgata acattctgaa tgttcttctt catgtcttca
     2821 agtttggttc tatccaatct gtaacctaag agtaattcat ttatttcatt caggatcctc
     2881 ttcttttcct cgattatccc gtcaatttgc ttaatcttgg cttcccattt ctcctttggt
     2941 agtctaagaa gttcttcata taggtttgtt atttcttctt cgttcacaag ccttttgcct
     3001 ataacatcat aaattttctc acctatagca ttcttaatac tttccatctt gtcaagcaat
     3061 ctacgaataa tgtatccgtc aatagtatca tttattacaa aattataata gtatacattc
     3121 ctatcttgac ctattctatg tattcgcccc attctttgct ctaacttgtt aggattccac
     3181 ggtagatcat agttaacctc tatattgcaa aactgcatat ctattccttc acctgctgca
     3241 tccgtgcata ccattatgtc agaaccttta gtatctctga acctaattag agcctcattt
     3301 ctttcatgta tattcattgt accatctata ctcccaacct taaactgtcc agagagcctg
     3361 tccctgaggt aattcattgt atctctatac tcgctgaaca ccaccatctt ggcatttgga
     3421 tcgcttttct ttgtggtctt gataaaatta attaataatt ctaatttcga atcagtttgg
     3481 tgcagatttt ctagttctat taagaggtca gatatccctt ctctttcttt ttggttactt
     3541 cctatagaaa ctccaattat ttcttcttcg tttctttcat aggcatcttc atcatt
//
LOCUS       DAOR01000091            9664 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_6903, whole genome shotgun sequence.
ACCESSION   DAOR01000091 DAOR01000000
VERSION     DAOR01000091.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_6903"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1681..1806
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8845..9005
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2921 a         1912 c         1985 g         2559 t
ORIGIN      
        1 atattgctga agtggtaaca gccaaaaagc gactggagat gcagaagctg agactttggg
       61 ataatattcg taccttggac gaacaggctc accgtgctgt tgactcaaac agagaggact
      121 tggcaaagct agctctggaa cgcaagaacg ctaacctgct tcaggcgcaa ggattggata
      181 aacaagttac agagatgcag gttgaacagc aaaagctaga agatacagaa aggcgtctgt
      241 ctaccaaagt tgaggaattt aaatcaaaaa aagaggtcat aaaagcacaa tattctgcag
      301 ctgaagcgca agtacggatc aaagaaaatg tcacaggaat ttcagaagaa atgactgatg
      361 ttggtatggc aatgaatcga gcagaagata aaacagaaaa aatgaaggca aaagcccagg
      421 ccttagatga aatgattgat tcaggtgttc ttacagatta cacatcaaca tcaacgacga
      481 gtggcagtgg taatgatata gagggggagc tacagaaaat gacagtaaaa agctcagtcg
      541 atgaggaatt agcaaagtta aaggctgaga ggctacgtaa aaagagatat ggacaaactg
      601 aggaagaaca gcaagcgcag aaatgaaagc tcataagagc ggaagaagat tttctagaat
      661 ggccaacaag gttccagtca aggctaaaaa acactcattg aggtcagtta gtaaacagag
      721 aactagggat actagtagga cgattttgaa gaagagtgat actaaactgc gtccaaggca
      781 gaggatagcc gccataaact cccagtttac cgtgaataaa aataaaaata aaaataacaa
      841 gaagatggca acaaaggctc atacccatcc cagtaaaata acgagagaca aggcaaaatc
      901 agtgaaaaag gtgagagtgg ctagaagaac cagaatagtg agaataatgg gacaaggtca
      961 gttcacagtc gataataaaa cacttaggga attaagcagg gtcgatagtt ctatagtaca
     1021 gctagtaagc agcgaaagac cagatgatac agaattcaaa aaacgtctat cgcagttaac
     1081 aaaaatcgta gagatgacgg ggaaaccact tagtcccaag gaaataattc aatctgatat
     1141 tatcttgcca agcgttgatc tgtcaatcga agaagccaag aagctcttca agggagaagg
     1201 cgtagtccca gaaatctaaa gaggcaaacc ccacagtggg taccagaaag tcatatcttt
     1261 ttcaaagtgg acctcttgtt cgactgctga taatatcttc atctcctgtt cggtatcccg
     1321 atttttttga ccagttggta tgaaggggta aaaattattg cgcttgtaat tgtaaatcaa
     1381 atactgaatg ctatcattat tgtccatgtt gctcctgaat tcgaatatgg ccgcaagtaa
     1441 cttatctgaa aatccttttt cttcaaccat atctccgaca gcagtcactc cggccagaat
     1501 atcttccatg tgtttccctt caataattac ccagagatat ccgtaaggat cggaaactgt
     1561 ccgatacctc agaccaaagt ctgttttact tgcgtcgaga aaacgttgga tatcttcttt
     1621 catgtcgaaa aaatacatac cgtcaaccac ttttatgctc accgccgacc tgcctgtgtt
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnntgcc tgtgtttttc aaacccagtt tagtctctaa cgtgatatac ccggatgata
     1861 gcgaaaaaat ggcgtcagag cttgcctttg aacggatagg attcttctta aatctcctaa
     1921 aaaaattcaa ctcatggaag cctccatctc catgagcttt tgcactctct tttctacagg
     1981 aggatgagta gagaacagat ttccgatcgt gcttccagat agcgcaggta caataaagaa
     2041 ggcattaaga ccctcaactt ctcttaggtc tttggttggg attcgtctca tagtaccgct
     2101 aatcttcatc aaagcgttgg ccaatttgac aggctttcct gtcatttgcg cagaccctct
     2161 gtctgcagca aactctctgt accgcgaaat cgcccttata atgagaaagc tgacaaccca
     2221 ggtcagcatt gcaaccaata atataattaa catagcaccc ccactatttc ggtccctgcc
     2281 gccgtatccg ccaccactat acaggccgcc gtagaaaccg aattgcataa gataccatgc
     2341 cacagttgag aacagacttg caagtgtcag tacaagggca tcacggttcc tgatatgagt
     2401 caattcatgg gcaactaccc cctctagctc ttctgtgtcc aaaatatcta gcaacccggt
     2461 ggttacagcg acgactgaac ttttctgtcc tctccctgtc gcaaaggcat ttggcatatt
     2521 tttatttatt acagctatgc gaggcttggc taggttgttt cttgcaacaa ttctttcgat
     2581 caattcgtgt aactgagggt attgttcttt tgaaactaac ttggcccccg aactccacat
     2641 aactatcttg tcagagaaat accattgcgc taaaatcatt cctgaagcaa ttattattat
     2701 aggaataaac ccaattccca gatgcgcaag cacacttaag aaaataagat acaacagcgt
     2761 caggattgca aaactaaaga ccattctaat ggtcagacca gtatctctct tctgccaagc
     2821 aggctttgaa atcgacgaca ttttcagctg acactatata ggcgggaacc acttttaatt
     2881 atttgcttga tcttgagata aagctctcta gcttgtccat tgcctcctca agcacatttt
     2941 ccggaggcaa gtatacaatt ctgaagtgac cagcaccaaa ttccctgccg aatccggaac
     3001 cttgtacggt taagaccccc gtagagttta ggagatcgag cacaaactct cgatcatcct
     3061 tccagcgatg ttctaactca atttttggaa atacatagaa agctccccgg ggcattttac
     3121 aggagattcc gtttattgag ttcagccgct ttacgacata gtctcttcgt ttgcgtaact
     3181 tctgtaccat ctctataatg tgatcttgtg ggccacgcaa tgcctggata gcagctatct
     3241 ggactggcag gttagacgag atgcgcactc gtgccagctt gggcacgttt tctcgtagac
     3301 catcgagctt tctggaatca ctgtttaggc atatgtagcc gcagcgccac cctgtcatca
     3361 agtatccttt ggaaaaacca ttcagtaaaa ttaccggagc atcacttgct actctgccta
     3421 tactggtaaa ttgttcatca aaaacaatgt tgtcgtaaat ttcatcgcaa ataacgtaaa
     3481 ggttatgttc gattgcaata tctattagtt gttgtaggcc tttacgatca aaaacttcac
     3541 ctgtagggtt attcgggctg attatgcaaa tagcccgcga ctttgagttg attttggatg
     3601 ccacatcttc gacgtcaggc cggccatctt catataactt aaactcgata ggttttcccc
     3661 cgtaaaattt cacgtacgat gcataaggcg gataataagg cccaggcatt agtacctccg
     3721 tgccggggtc aactattgag gccaatgtca tatcaagacc ctcagaaacc ccattggtga
     3781 ctaagacgtc cgagtcggcg agtgggaatc ccttttgaga ttctttttct actatggctt
     3841 tccgcaactc cgggagtcct tctgactcgg cataatagtt ttggtctttt gttacggcgt
     3901 ctattaaggc agctttgata tggtcaggtg tcttaaaatc gtactttact ggatctccga
     3961 tgttcaagta gattatctgt tttcctgtac tttcatattt tcgtgcatat gaaattatat
     4021 cgcgaataac atattctact ccagaggttc tatcagagac tttcaaaagt gggatcaaat
     4081 ataagggaaa tttaccaaaa taaatggtta tgcgttaact cgtttaactg gattatgata
     4141 tacaaaggca ctttttggct agctaccaac taccccgtgg cctctgttgc ttacggacgt
     4201 cattgtaccc attgtttgtg aatttttgag cgcgtcagct tgggaaaata agtaaaatca
     4261 aaactagccg ggcccggagg gcttcgatcc ctcgatctgc ggttccgaag accgccgcga
     4321 tatcctggct acgctacggg cccatgcaag tgttgaactt gtaaacccat atatcaataa
     4381 atcttacaat ggcgtcagca ttgcaaaaca ttagtcatat cggccttcac gttcatgcca
     4441 atatcgtagg ttctgtttat atcatagccg gtatatggac ggaactttat ccacgagtat
     4501 tcaaaaatct gttatgatgt cataacgtta gatggtttaa tctgtcatgc tctgctctgc
     4561 tagatcggat ttttatcttc taaatgtttc agtggagtaa agaattctgg attataaaat
     4621 tcggggtgtc gacgccatag aacattagac ctagggaggt cgagtgaaaa tcgccgatgc
     4681 atgaataata acatcaatat taataatgcc aaagacacca ttcgccaata tgattttatc
     4741 taaaatcgat cttggaccat actagtggcc gatatcatag tcctattttc tgacagaatt
     4801 ctattggaat tcagatggcc ggtgctctga gagttagatt tcgaacacat catgttcggc
     4861 agtatttgca cctccaagtt ttctttcagg attcacttca tctactcgtt tggcaagttc
     4921 cactagtttt ggatgtgaaa gcaacttgtt gatttcctca agctctgtcg ttgacctaac
     4981 gactacgcct ctcttctttg tcgcagcacc agaagacccg atagggttaa cttcaatcgc
     5041 taacgatgcg gttcttccct tgaatgaagg taattttaat aaaaagactc ctggtacgtt
     5101 cgttgctttc ctctcccagt ccttgccctc cctcaaaaac tgaatcaatc tatcatcaac
     5161 tgttatagac ttcgtccccc tatactaaat ataataaata actgagagac gagtgttttt
     5221 taaaccttgt cctttaactc aacttgtatg gcgatgaaaa aacaaatatc acatgtaaga
     5281 aaccggtgca gatttctacc tttcacaata cagggctaaa caaaggttaa aattttcata
     5341 cggctattag ttataagaat gtccagggct atgctgagct atgataaagg cacaatatcg
     5401 atacaaggtg tagcacatat tccttattct aatttagatc cccgcacaaa catgctgaga
     5461 gcttttgcac tatattctaa agattttcta gattatcttg tgaatgtgaa tttgagtgat
     5521 tgacggataa tcgtttgact tgcaagataa aagatacaac gcaagtgtga catgacatgc
     5581 ttccgctcca actactcaga gtcaaaattt cagataaagg gaaaaacatt gccccaatct
     5641 tttgtacctc ggatgaagac gtatcgttgg cagctgatat aatcggagag ttcgaagaat
     5701 catggaagaa aagagataga aaaggactcc ttacagatcg aattggacta atcgaaagta
     5761 aacacggaga ctataaactc gtcaggggca tttctacctt acttgaaaga aggtgtacgt
     5821 ttcttaacag tagtgaacag tcaaggaaaa atggtattga ttcggttcac ctcatgattg
     5881 aacctgatat aataaggcga agtttattcg aagaatcatc taaatctgat tttgcattaa
     5941 ctattcttag aagagaagag ataattcaat ctgtcgcgag gcagctaggt gtagataagg
     6001 aaaatgtgct aaatcaaatg tggagtgacc tagatgagaa cttagttgtg gagaatttcg
     6061 atggaataac gccggcaatc cttgtggcat ggtataacct cgccctcata cagacactct
     6121 tatttagctg cacaactcta caagtcgctc tacatggagg gtcgagctgg aaacgagtgc
     6181 taagagatgt caaaagagta ggcctaatgt ataatattcg aaaccaacca agcacattag
     6241 tcgatcggac ctctaaaaat acgatttatc atggtgatac agttgtgtgt tctctcgatg
     6301 gtccacaaag catgttcaag gtgacggaaa tctacggcac ggcgattgcg aaattacttc
     6361 cgtctataat ttctgcagaa tcatggtcgc tgcaggcatg gattatcagg aagtcagtat
     6421 ctgtaggaag gaaaatgtat gaatttcaca tctcaaaaga ggatgcacct caattaatgg
     6481 cattgcctgg tcataacaat agtaagaagc taactgccaa atctggttcg tcctttgata
     6541 gcactgttga agaaaaattt gcgataaaat tccagcaatt tcgcacaggc tggaccttga
     6601 taagggagcc ggacccgatc attttggaca atgataaggc attcatccca gactttatgt
     6661 ttgaaaggta cggccgtcgc atatatttag agatagtcgg cttttggact acggaatata
     6721 tcgagcgaaa gatcggcaaa ctaacacgaa tttcttctgc gaataagcaa ctgtacaaag
     6781 atgacaaact ggatatcttt ttggcagtga atagcgatta ttacaccgct tccaagtata
     6841 aaaaagtcga aaaggatctc atctcgagac tgggttcatt agtacaaaag gatcatttga
     6901 tactttataa aaacggtcaa gtgcccctga aggcaatcct ttcgtacttg aagtcaattg
     6961 atcaggagat gattcgaagg atagcaagca ccgcctcgaa tgatttgcta gcagagatag
     7021 accgtcttac cattggtatt actcaggata ataacgaaga cgatagcaga actgatggag
     7081 gcagcttcgg cggcatagta tcgataggcg agttagctga aaaatataat atcccttctg
     7141 attcggtgct aatagctctt cgatctaggg aggcaggaaa tgtaaatgta aatgataacg
     7201 ggcgtagtta cctaattgtt ggaacatatc taatatcgcg ttctaaaata ttggaactag
     7261 agaaatcttt ggattctatc tcaaggttca tggatgctat tttccttttt aaaaagtata
     7321 atgtacccga atcttgccat gtcgatctcc tttcaaaatt aggctatgat ataatatgga
     7381 gaggcataga ttcaactaat gcatcaattg aaacaagaaa aagataggtc gctgcagttc
     7441 catcccgagg atcgagatag tcgcgataat cggtaatcag agcacttagc gaggtattag
     7501 gtacatggta tttgagagaa aaaagatagg acccccctac tgcagctaat gtttttaata
     7561 aagattcacc atgattgtgc aagccgaggg gtcgttgtct agcttggtat gatgccagcc
     7621 tcgggcgctg gagatcgtcg gttcaaatcc ggccggcccc attagtcaaa aaagaagtaa
     7681 aatatgcaat atagcacaag atgaattggc tggaactgtt tcatcttcta gatttaaatt
     7741 atcccaaacc actaaacttg atatggcgga agcacaacca caacaaaacc cacaagttca
     7801 tagagatggt ccacgcgacg cggtatacat aggaaagaag cctttgatgg catacgtgac
     7861 ttcaacactc atccaactag ctaatcagcc gtcggtaacg atcaaggcac gagggttaag
     7921 cattggtaga gcagtagatg tttcacagat tatcctaaag aggatggaga acgccggcta
     7981 cagggtaggc gaggtaagaa ttgggtctga gacggtgcaa tcagaagacg gcagaacacg
     8041 gaatgtctcc acaatcgaga tagaagtaaa gcgagtgtca taagggcctt ttcgaccctt
     8101 tttgttattt atatgtccgc tagcgtttac tataactgat ggaaagagtg gcggcgatct
     8161 acctagcaca cttgaatccc tttaccaata cccatcgaca tatcatttct ctattattgc
     8221 agcgagacta caatgtttac gtttatcccg taaggttttt gaaaaatcat aaagagatca
     8281 atacacgcag tttccctttt ccttatcaca taaggaaggc aatgattgaa tcggttttcg
     8341 gaaataataa gtccatcacc gtttctccgg attactcgct agtctcgcca ttcgtaagat
     8401 atttgccacc cttaatttct ccattctcct ggatattaag aaatcaaatt ctccgaaatg
     8461 ttagggaaga aaagttcatc acatatacag gggatgttgc tgaaagaatt actttgtctg
     8521 tctttaagct taatcccata aaagcccgtc ggtttgcagg ctcagcttct gctgtgaagg
     8581 aaatgctcta cgaccaaatt actcccaaat tttcaaacgg ttcgcgcagt gaacaaagac
     8641 atgaggaaca aaattggcgg gatatggtac caagcggagt cgcagacttg atcgagcata
     8701 attggcagat tgttgagaaa tttgcaagat catccgatag caccttgagg gtgatgggga
     8761 tgaaaattcc aaaagaaggc tttgtttaac ttttgaagat ttttataatc cgcgaattga
     8821 ctctgccata aatgagaaca aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnncgcca taaatgagaa caaaaggcgc tgacttcatc tggattgatg gcaaaattgt
     9061 aaaatgggaa gaagcgaccg ttccagttac tactcacgct ctacactatg gaacttcagt
     9121 ttttgagggc attcgtgcat attcatcaaa aaataatctt catgtattta gattgagaga
     9181 acatatggac agactccgtc actcggctgc agtctattcc attgcaatga attattcagc
     9241 aaaggaatta gaggaggcaa ctttagagtt attgaaaaaa aatcggatga aagaatcatg
     9301 ttatatccga ccgttagcat ttgtaggaca gcacggtatt gatttgaatg taacgaggga
     9361 ttctcctacg catgttgtga taatagtatt cccatttgcg aagtatttta aagtagaagg
     9421 cattagtgcc tgtatttctt catggcgcag aattcatgat tcctccacac cgccaatggc
     9481 aaaagctgct ggcaactatc ttaattcagt acttgcaact caagagagca ataggaacgg
     9541 ctatgacgaa tcagtgatgc tggacagaga tgggaatgtg agtgaagcgt cgggagagaa
     9601 tatcttcatc gtcagaaaca acaaaatata tacaccatat atagccggat ctgccttaga
     9661 aggt
//
LOCUS       DAOR01000092           25295 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_2287, whole genome shotgun sequence.
ACCESSION   DAOR01000092 DAOR01000000
VERSION     DAOR01000092.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 25295)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25295)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25295
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_2287"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1007..1278
                     /estimated_length=272
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10271..10464
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7768 a         4772 c         4721 g         7568 t
ORIGIN      
        1 atacaattca gcatgctcct atactgccaa ttctataaga acacgccaat cttaatgtct
       61 aatcatattt catcattcta aaaaactcat gatgctatct aagcagataa aataagatga
      121 gaggtatttt cgatttaatt tattttgttt tagtgttgga aactgattca attgtcaata
      181 caccttgttc cttggttatt cttcaagatt aactcaaact acatttaaca gtccttaaag
      241 tgtctatttc ataacccata gaggatttgt ctttttcatc acggatagcc aacccatatc
      301 cggtggctgg atatccattt gccatagtat ggctattatt tcacctctgt gatgtagttc
      361 ttcggtaaaa acatgaatta gcacatcttt tacaagagat gttcttctta taccatcatt
      421 atttactcta aaaaccggct taagtaaatc ttcttcctga ttatcggata aatatttctt
      481 agttttcgaa gtaacctcct gattgtaatc tattaaagcg ttccacgtct gatacttatg
      541 atatggaaat ggtctatttg gatcgtcgag tccttggatc gaataattaa tccatgtatc
      601 ttctacccac attatgtgaa gtaatgtatc cttcatagat aaccaagcag cttcacgatt
      661 cttgatcatt tcttcccatg aaataatttc ttgaaaatag tgcagatagt tcttccttac
      721 ttgatggtta tactcaaata acccccgaaa tcctccaaat ccattggcaa actacatgta
      781 accgatgcga tgagacaaat atttcttgct ttgagtctat gagaacatat tattattgtg
      841 gtgtagtaag tagaatcggc atttcatctc cacatggtga tacgcataaa cacatttctg
      901 cttacctatt tcttggtggc tctcggaaac gacgagccaa ttgttgtgat aacaaatata
      961 cactacatgg aatctgcttt tagcgcaaga ggaaaaacaa tttcttnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnntt gaatcctcaa caaatttttt taattttttt ggctgattct
     1321 tcttgtgaaa agtttttgag ttgagaaatg gttttgtcga ggaattctat aatctgtcta
     1381 tgttcttgca tcaccttaat agattgttcg gattggtctt cggccttttg cataataaca
     1441 cacatgattc ttgcctcttc ttctactgca tgtcgaagaa ttgatttacc taaatcactt
     1501 aacctctcta ctgcttgttt tgaattgctt ctattcttct cctctatttg aagcaaattt
     1561 gattcgaaat catgatgctc atgctttaat ctttcagtct tttttttccg ctccccccct
     1621 tccgtttttg atatttatca acctatctgt agtgtccgtt atcagcaaga tatccgtatg
     1681 ttccctacct gctagagaac tctatattaa atcggaattc acaatcatac atatgttgaa
     1741 aaacagattc aagaagatcc ttgttgcgca cgatgggtcc gagaattcta gaagaggaat
     1801 gaacgaagca atctcacttg ccagacagtc cgacgcgata ataacaggcg tacacgtact
     1861 acatggaggt ttgagtgaat taaaaaatac attcacgcac tacatatagt atctattgga
     1921 aaaagcaagg aaattcatgt ccaatgccaa aaccagcgct gccagacatg gtatagactt
     1981 tgaagaaaaa atcgtaacat caaccgatac tataaagaca ataacgaatt ttgcaaagtc
     2041 aggtaaattt gacatcattg ttataggagc aagggagcag gatctccaaa atctgaattt
     2101 tttggaagcg tgtcaaactg tatacttcac ggctctcgcg tacctatacc tgtagtaaag
     2161 taatttttca aacttatctt attttatcga atagtggatc agtaacaacc atattctaca
     2221 tatgtttttt ctgaatatcc aatcggtatt taggaataat agccatgagg tctataactt
     2281 gtgtttcata ttctacattc tttgcctatt gggttgaatg cgacaattta ttagttgttg
     2341 agaaggttta gtgaaggtga ccaaagtaaa atgagtaaat gccctgaatg cggggctaca
     2401 atgtttcccc gcgatgagaa agatgcatct ttagtggaac atttgaaaga ccaacatagg
     2461 gtcgatcatg gatctgctct ggcaatacga ggtttacaaa gagaaattga tgaattaaag
     2521 aagagattag actcattgga atcaataaaa aagtaagcaa gaagcaaaaa tagagatgcc
     2581 atatcattta cggctgacct ttcctaaaga cgatttaata ttgcttattg gtcggggtaa
     2641 gcagtggtga ttgatttttt gtgctttgct ttataataat cacatatctc acgcgaagtt
     2701 acagccttca tcttctctac cttgccatct ttctcgtctg gcttagtttt tgaattgtat
     2761 aaaggaagaa aatcttcctt aagaaacttg agcttgtatg aaggtatccc agttagattg
     2821 taagtcatgt aaagtacttc ttgcaggtcg gttaaatcag caatagggga ttgttcaatg
     2881 cccattatta gtttcatcct actttgagaa cttgctaacg ttactacgtt gagaaaagca
     2941 aaaatgcgtt tggtcattct gacatccgtc cctttttctg caggaagtgc ttcagcaaga
     3001 tagtcagagt atggtatcca tattaaattg gattctctct taatgtcaga cgctattgtt
     3061 atgctagtca attgtcgcat ttcatctcta acgtacttgt agcattctct tgctaactcc
     3121 acttcttcat ctgatattac aagatcctgc tgtatgcagc ctggcaaccc attttttatt
     3181 gcaataagca tattggattg ttggtatttc agtgtgttca tagttggtga agatatcaag
     3241 aagcggctta caatttcgtc ccagtcatgc caataacttt cgtctctagc tgtacagaag
     3301 atgcaagcag gatatccctg cataacaaca cgctttacaa atatgccctc tttatcattc
     3361 ttgtcaacat aaggatactc aatgtgatag ctgtcatgag atagaattgg cttgagcatg
     3421 ttccataatt catgatctcg cggctacaaa aaaactaaag ttgtgccacc tagcttgatt
     3481 aaatatctgc acttctctat taactcctcc ttctgttcct ttagttttcc cgcagtttct
     3541 ttgtcatggc gatctttggc gccttcgatc tgtccatcca aatcttttag ttgtggtctt
     3601 aatgtttgtt agattcatca acaagcacgc ctttttgtct taccaatatt tttggagaca
     3661 taccaccaac gagccacaca tcttcctttg gcacatattt taaagtccta gtaaccggat
     3721 atgtcttgcc ttcacttgta ggtgctataa ttcctaagtt gataggatta ttggtgttag
     3781 cgcttaatat tgtgtaaaat atttgtctaa gtaacgaatc ctcctgcatg acttcctttt
     3841 tttacacatc ttattatgta ttcagcgtaa tctttcccag aatgcccata attactgcta
     3901 cagcgccaaa tagcatccat gccttagccg agtacaaact gcttttagta caacttagga
     3961 cttattaagt aggagtttag atagtaaaaa ccatatccct atctctatcc tagtcttgag
     4021 ggatcgaaaa ttatgtcaaa tttggttcac gcatccgcat tggccccaat aattacatgg
     4081 ggtctaatat tggaattgga tagcatacta ctacagcatg cgcctcaata atatggcgct
     4141 attaatggta taaataacct gaatataatt tattttattt tgttcaaata tatctaaaat
     4201 tatttatgct tagctatgat cttgttctga gcgtgaatta cgacaaagtc tataacaaag
     4261 taggatgatt gattatctat tttgaacgta atattcagat aatgattacc tccagctttc
     4321 cgttttctac ttcggttttt ctgctctcaa taacgagtac attaatgcac ctgaggcaac
     4381 aactagagat aatacatatg cggctataat tgcctccata ctacgattca cccccctccc
     4441 aggctggtga tacctgctgt tgttttttga ggatctgcat tattcgagaa cgcactataa
     4501 acgtcaggat attgatttac taattccaag ttaacttcca atacattgac atagtcagga
     4561 gcaaatgcca ccatattgct gactctgttt ctttctacat tgagttcaat taatgttgtt
     4621 agcgcgtttt gatttatacc agtagcttga cttacattcg aaatttgcaa caaggcattt
     4681 tcaggagtta tatccggatc cacgtttgat gctgactctg ttgaaggcct tggatgaaag
     4741 aactttggag atttaaaatc ttgtgctata agttttgaac caattacttt tccgttaacg
     4801 gtaattaagc tcccatttga ttgatatggg agtgtacttt gacctattac tactagaacg
     4861 agagggtaaa caatacctgt tactactaac atcagtatta ctactttgat tggaggacta
     4921 agtatattct tatttatttg actttttatt tgctctaaaa ttttcaatcc tttttcacct
     4981 atcatattca ttatcactat tattcacctc atgaaaagtg ttagcaaaag gtcaatagcc
     5041 tttatcccaa cgaaaggtaa agctagtccg cctaggccgt agataagcat atttctaatg
     5101 aaggttttct caggcggctc tggtctaaag ctaacacctc gcaaggataa tggaattagt
     5161 gctggtatca ttattgcgtt gaaaatcaat gtagccagta tagcagtcgc aggactatgc
     5221 agctgcatta cattaagaac tgctagcttt ggattactag tcgcaaatat ggcaggaagt
     5281 attgcaaaat atttggcaac atcgttagct atactgaatg ttgtgatagc tccacgtgtc
     5341 attagcaatt gtttgcctag ttttaccact ttcaatatct ttgaagggtc agaatctaga
     5401 tccaccatat tagcagcttc ttttgcagca gcagttccgc tattcattgc tagtccaatg
     5461 tcagctttag ctagtgccgg tgcatcgttg gttccatcgc ctaccatacc tattacatga
     5521 cctttggtct gttcttcttc aactcttact aatttgtctg tgggttttgc ttctgccatc
     5581 acttgatcta cattagcctc ctttccaatt acttgagctg ttagctgatt atctcccgta
     5641 agcatcacag tagtgatacc agtagaatga acttcgtcaa gcttctggcg tatgttttcc
     5701 ttgaggttgt cttttaaagc gacaagaccg attgcttcac tatttattgc tattaaaagt
     5761 ggtgtttcac caatcttaga tacttcttga gctttccatt tcaattcagc ttcgttagca
     5821 tgcgtaagaa attttagcat ggcatctaca gaacccttaa gaatctttac ctcttcatta
     5881 cgctgctcta attctaatag catgcgacta actctagtac tagcatttcc attagtgcta
     5941 acttgctgtt ctgcatttcc atcttcaaac ttgtgaattg ttgaaaccgc ttcttgttgc
     6001 tctgtactga gtgccaatct tttgtcctgt ataaattcaa gaccgctgaa tctagtctcg
     6061 gcactaaaat caatttgtct gccagtaagt attttttcta ttaatggggg aatgaatctt
     6121 ttctcttctg ctagatgtac tattgatttt ccctcgtggg tgatatcatg atatgatgct
     6181 gcaaaagctg cttgtcctac atctttttct gcgtattttt gcaacggtat gaattcgttg
     6241 gcacttctac ttccttctgt aatggttcct gtcttatcta atatcaaaat atcacaatct
     6301 ccagctgctt caataccttt tccagatttg gctactatgt tatctcttcc aatcctagta
     6361 ataccagcaa cacctatcgc agataagagt cctcctattg ttgtaggcat cagagctact
     6421 agaagcgcaa tcagcattgc aatatcagca atgtatccaa gaaagaatgc aaagaaaagg
     6481 aggcttccaa caaccattat aaatattaat gatagccctg ctagcagtat tgttaaagct
     6541 atttcatttt tggttttagg tcttgtagca ctttctatta aactaaccat ttcatcaaga
     6601 aagcttctac caacctccgc agtgatttca attcgcaact tgtcacttgc tattcttgtt
     6661 ccaccaatga catgatcatc cttatctttg aatatcgggt ttgattctcc agtcatcatt
     6721 gattcgtcta caaaagcctt tccttcacaa acaagaccat cccttggaat tatctcacct
     6781 acataaacca gtacttcatc ccccggtttg agactggtag aagtcacgat aacttctttc
     6841 tttccctcgc ccactatttt cctagccgaa acttcttttt cgaggcttct tagagaatct
     6901 actcgggctt tagcctgagc ttctgatagg gcttctgaaa atgtagcaaa ccagactgta
     6961 ataattagta ttactgctac ttcataatag aatatcggag tttgactagc aaactctttt
     7021 ggtatagtcg ggagcgctag accaatagca aaaactagta caaatccaac ctctactgca
     7081 aacattaccg cattattttt tgctaaatag taagggttta gttttgtaag ggaaccaagt
     7141 attacttgtt ttctatttat attcgataga tcttttgtaa aagtagaacc attattttta
     7201 ttattcttgt atttgaattt ttgtctcatt ctcattattc ctatttatcc tatttccatt
     7261 acattgaaaa cggtcctatt actatgaatg ggaagaaagt tagtgctgtc aaaaggaacg
     7321 tcatgaccaa taatactgaa ataaacaacg ggccgtgtgt cctgattggt tcgatggcat
     7381 cttttctatc tttgactgtg aaggagcctg cgatagccag catcaacact attggcacgt
     7441 atcttccaag aaacataact attgcagttg accagttcca aaagggtgtg ttggcagaag
     7501 ttcccaggta atcagagcca ttattagctg ctgcagatgt gtattcgtat aatgcttgag
     7561 tatagcctga ggctgttgtc atctttcctg caggaacgcc taacacactc tgagcatttc
     7621 cagtagtaaa cgcaattacg gttggaatta atattaaagc aggatgcatt aagaatacaa
     7681 atacagctaa tttgacattt tttgggttta ttttcatgtt cataaattct ggagtcttgc
     7741 caaccatcaa gccaactaga aagagagtta tgattacgta gatgatcata tacataattc
     7801 ctgtaccatc agctccagga acggattgaa taaacatacc aagcagaaaa gacattactg
     7861 gatttggtga cattccactt agtgctgaat tcgctgatcc agtattcgta gatatagaga
     7921 cggtattgaa aaattcggta ccgaaactgc caaacctagt ttctaaaccg gtaggcccac
     7981 ttacttgact gaaggctatt gccagcaaaa ttccaaacgc gataagtatg gttaatagta
     8041 atgatactcc tcgagccttt cctaccaatt tggcgtacgc aataggaaat gacaaaggaa
     8101 ttatcaacat gagaaacatt tcataggtat ttgaaagacc attaggattc tcaaatggat
     8161 gaccggaatt tgcaccatag tatccgccac cattgcttcc aagatcctta atagattcca
     8221 aagaagcaac agggcctgtt gtaatggttt gattatgtcc ttccaaggta tgattgatcg
     8281 taaccgatga ttgcagagtc tgtggaactc ccaacagcat aagaagcaag gcagaaccaa
     8341 atgcaacagg aagtagtagt gttaaaatta ttcttataaa gtcgacatag aaattaccaa
     8401 gcccaaaatt ctttcttata aaaccgcgga taaaggcaaa cgcagcagca atcccggaag
     8461 caggagctac aaacataaca aaagtaagtg gaatcatttg ggatattacc gaaagttgct
     8521 ggtcgcctgc ataatgctgt agatcagtat ttgtaacaaa tgaaatcgca gtgtgaaagg
     8581 ccaaatcaat tgaaagccca ttcacatgtg ttgaagatgg aaaaaggttc tgaaatacta
     8641 acataaaaaa agtaaagcct gccacaatga cattcgtaag cattagggca agcgaatatt
     8701 ctttccatgt catctgtttg tccttatcta tcccaattaa cttgtaaaat ccattttcga
     8761 ctcttgcaag tggagcttcc aatggtctca attcatagga tatcatcctg gcaatataca
     8821 gtccaaaagg aactgacaaa cttaatgttc ctccaaaaac aacaattaat tctactattg
     8881 gattcattaa attaaattaa caccatcctg ttctttcggt agagataaga cgcatccaca
     8941 aacctctgat ttcatcccat ccaaaaggaa caaaaagcac tgataggatg cttattagcg
     9001 gtagctggtc agcagcaacc ctttttattt tgtccacata ggtcactcac taaatagttg
     9061 tagagttgct tgaacctcag atattaatag tttatgagcc aaatacaatc agaatattat
     9121 attctctcct caatatttac aacatttttt tattaaatta atcgtagata agaacgaata
     9181 atgtaggttt ataatctacg atacaaattc gataacaaat ggtctttaca attacagatt
     9241 tatagctaat ctcacgctcg aaacaagata atctaaggcg agcattaata gcgttacaaa
     9301 tccttcaatc aaataaaata aattatattc aatttatctt aatagcagat aatttgcaat
     9361 taaatacaat gaatcttctc gcggaaggat gatagaaact cagttaacct atcaccttac
     9421 gctttcagca acatcctcaa attcaggtaa taaatcaact aaaaatcaat ttcaatttaa
     9481 ttcgacatga ttccaccgcc ttaactttga ttatcattta aaacctaatt tcacaataat
     9541 taaagtaggt cgcgctatca tcttcgaata agttttcaac ccgtcaacaa atatagcgat
     9601 agtttgactg tagaatatca taacgcctta tggcagcatg gtcgagtgtt ggataaggca
     9661 tattaataat attgctagag ataagctgaa gctcttatgt gcatgatttt tctggaaccc
     9721 ttggtacatc ttctcttaat ccgaattcga tacggtctag tatgatatat cggatatgag
     9781 tgctgtttca tgtatttagt tatccatctc ttctcgcatg atccgagtca atagactaaa
     9841 tttgatatgt gatgttctca aattatacat gaatacaaac agtcgtagcc atttgtatac
     9901 atgcggtagt tcacccttta tacacggata ataatcgtcc aaattttcag ttctatcagt
     9961 agcatatttc gttactcttc caataatatt tttttttgaa gcgatgagtg agtgtagttg
    10021 attttcatgg taataccatc ttccaccatc tgaacataga atatgtctac caaatagctt
    10081 agcctaagat ctaagaaatg attctataac tggcatgccc cgacatcaat agaaaaagaa
    10141 acaaatgaat ttaattcaat agaattatta ttctgaaata ttttatactg aaaaactttg
    10201 caaggcaaaa tgctctacta aggtgtcttg gctacggtaa atgtaattta gccccttagt
    10261 atacggcaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnntatacg gcagaaataa tttgttggac atcagagact
    10501 cccatataac caagtaatcg aacaaaagaa tctggacatc tgcctgatgc acttcctacc
    10561 atcgttcatc ttactaaacc cattattcca attgtcaatg acatcaaaat caagtactgt
    10621 ttcactacgt cttaccaatc attcgttata gataccccag ttaaccacca tagatgatgg
    10681 tataccacgt aagctaaatg caactaggtc aaaaccctat caacagaaca gtgagttatg
    10741 cgactgctgt tttcttgttt aagagacgag tagtaaatag atccgccaca aacatataaa
    10801 ttctggtttg tcttctccta agaaacaatt agtgagtgac ctatgtggac tcgataattt
    10861 tccttgccac agctatcggc gcaagcataa ctatagtatt actttttaat agaacagcac
    10921 tgtcgatcaa atggtggaaa cgcggcatca tcataggact aaacggaaac ggagatagtg
    10981 ggaagcaacg ctttcaatac atcaaagaat ctggagtaag atcagtagta cgatcaaaac
    11041 ctcgaaccca ctctctcgaa aagctaaaac aaacatctaa ttggaatgag ctaataaata
    11101 agaaagttga tacctgtgag atgatctaca ttggatgtat agttaatatc gatggtcatt
    11161 caatgactat aagaagcgac agcgatcagg aatatgtcat agctacgtat tggatcaggg
    11221 gatacgatca agaaagagca gtgatagata catcaattag atatttggac cattatcaag
    11281 tcaaatcaag aacaatatga acataaagat aggaggtgtt ctctaaatag tacctctgac
    11341 tattagatcg aatcgaaaca tatttttact tatctatcaa gataatggaa ccataccaag
    11401 agttatgatt tagtacgtcc tggtggttac gttgaaagag cgggtgtaac attgtttgcg
    11461 tgatgcagaa atccatgagt gtgcatcgga ttcgtcttgc agccgtttct ctggctttgt
    11521 ccggaatatt ttttgtcctt tatacggcga tccgaccttt ctccgatgaa tcatccttgc
    11581 tgggggctga ggcattcgcc tccttctcat gggttctggc gcactcgcta gctaaagcgt
    11641 ttgtacttct agcgttgggg ttgctcggtc tgtacatacg tacgtctaca agaaacaccc
    11701 gttgagcgtc gcgcgatcca agcacttgtt ttgagctgga taggggtcgg attgaccctg
    11761 ccctactata gagctgagac ctgattcggg atcgtgtcta tatgcgcaga ctagcgggcc
    11821 gtgatagcga ggacagcgag attacgttct taaacgggcc cagagaatta gacgaagatg
    11881 attcaagtaa atggatatgc agcccagcaa gccaaggcgc ctcttacttc gtactctttt
    11941 gagagacgtg agcctcgcga tcatgatgtc gccatcgaca ttcagtactg cgggatctgc
    12001 cacacggaca tacaccaagt acgtaatgag tggggagcat cgacattccc gatggttcca
    12061 gggcacgaga tcgcaggcgt tgtcacggga attggcgcaa agtcaacccg ttataaaatg
    12121 ggtgacatag tcgctgtcgg ttgcttcgtc gattcttgcc gcaaatgcgg tccttgtcgt
    12181 gaagggcttg agcagtattg tgtggaggga ccgactttga cttacaacgc agtcgaaaga
    12241 gacggaaaga atcgtactca aggaggatat tcgaataaaa tcgtagtcga tgaaaattat
    12301 gtgcttcgaa tccctgagaa tattccaatg aatcgtgcag ccccccttct ctgcgccggt
    12361 atcacgctct attcgcctct tatgcattgg aacgcagatc ctggaaagaa tgtcggtatt
    12421 atcggtctag gaggcctggg acacatggga gtaaaaatcg ctcatgcact tggtgcggaa
    12481 gtcacagtgc tcagtcactc gcttagaaaa caagaagatg gcaagaagat gggagcggat
    12541 aaattctatg ctacttccga tccggagact ttcgagaagc tcaaagggta ttttgatctt
    12601 ctcatcaaca cattagcagt cgagatagat tggaacaaat acttgaatct actcgcatta
    12661 gatgggacca tggtcgtggt cgggattcct gagaaagaga cgccgattgg cgcctattcg
    12721 ctgattagtg ctcgtcgaag tcttgcaggc tctgtgatcg gtggaatacg agagactcaa
    12781 gaaatgcttg atttctgcag taagaataac atcgcttgcg acatcgagct tatcccgatt
    12841 cagagggtga acgaggcata cgagcgagtc ataaacagtg acgttcgata tcggttcgtg
    12901 attgatatga agtcgctcga gagcaaggac tgattgcaca ataggatgat cttatacact
    12961 tcattttaac aagtaatagt cttccacttg aagatgccat cattattagt caattgatcg
    13021 gtcaaaatcc gtttgtactt atgtagtagt acatctagca taccaactct accaagtgac
    13081 ggccgcggtt gacgggcgat ttgacgacat tgggaaagag tacaaaacgc gcttgatcta
    13141 ttcaagtaat aatatatctt cgaaacaaaa aaggaagagg aaagatccct aattatccag
    13201 tactgtttag cgttatcggt tttgcaaatg tacttccacc atcattactg gctctgaata
    13261 gtggcattag tactccggtc ttgttggtcc accatgttac atacacgttg cttcctgatg
    13321 ccgctatctc tgtattcaga tctactgcgt gtcccttgtt gggggagctt atcatcattg
    13381 tcttgagtgt cttgccgccg tccgtgctct ttgcaaagaa tacgttccag tgtcctgtgt
    13441 cgttgtttgt ccacgccatg aagacagtat tatttcctgt ggcagccata ggaacccccg
    13501 ctctcgcaac caaaaaagga gatccatggg ttggaccttg aggaaaggca cttaccgcat
    13561 ttgacgaagt aatggtcaca acggccgcat caagcaaaaa cactgtcgta agcactagta
    13621 ctaacgttac accaacgcat tttttagcga tggattcgtg atttgatatt gtctcatgat
    13681 aattgagcgc atatttaagg acgaccagaa aatcgtttta atatataatt taaggaatcg
    13741 ggcttaagtg gtaccaatga caagtgacat tgaagcagct cgccattccg ctttacgttt
    13801 tactgatgat ttccttttta tttttcagtc tgatgttttt ttattgacga acagctaggg
    13861 ggaacaaccg gtttaacgcg atcattattc gcatggttac gtcggcatta cattatttaa
    13921 tgtgaaatta tttctaatta tttaagaaat acaacaacca gagactgtaa aagcaatgtt
    13981 aatctagaat tgatatataa cttcattaaa catctagttc gcaatttcac aggaatacaa
    14041 accaattcag gtattccaac aatgcccaca ttcccacatt acaataacta cattgactaa
    14101 attacaaagt cacgcaataa aggagcacca aaattaaata tttcaacatg tacgctatcc
    14161 cggcgcgtat catatcagtg ggggcattca ttcccaaaca atatctgccc cgcaaatttg
    14221 ataaccagtc ggctttattg ttttgctaat tctcatcgcc gtagcggatc ataatatgct
    14281 tggttgatgg atgcaggaat ttttagaaat cccagcggag ggggattaca cgggcaagat
    14341 tattacactg atatggctaa gtcttcagat cttcttggaa agacaggcaa aaggaaaaca
    14401 caatgggtga agtctggaat aaagtctaca aatcagacaa cgctttcttt ggacaggatg
    14461 ccagcaactt tgccctgctt tgtcttaatc acatgaaaat gaataatgta aggagaatct
    14521 gggagctagg cgctggtcat gtaagagata caacactgtt tgcatcaaat ggaattgaag
    14581 tagaagcagt agactactct attgttgcta ttcagatact ggataagata acaaaggaaa
    14641 aaagactgcc aataacacca cgaatctttg atgtcaggag agcgcttcca tttccagata
    14701 gctattttga tgctgtgtat tcacacatgc ttctcaacat gcgcttttca ctcgacgagc
    14761 ttcattttct attttcagaa ataaggagag tactcaaacg aaagggattc aacttctttt
    14821 ctgtaagaaa ctacaatgac aaatcctacg gcgagggggt ccaaatagat aaaggaatct
    14881 atgatataaa cggctttcaa gtccgattct ttacagagaa ggagatgcaa gatttaatgg
    14941 aaggctttga aatactctgg atgggagaag aaaatgaaga atcagtaatt tgtatattgt
    15001 attcagcaag aaaatgggaa tgaatttcgc tacccctgtt cctatggagc acaacgcaga
    15061 acatatggcc tagtcattat ggtcattcac ttcaatatca aatgtatccg ccttatcttt
    15121 aagccacttt ttgaatttac tatttgaaaa atgaaagaaa tgagcaaaga agaaattcaa
    15181 acctcccttg tgtctggcac tctaacggga aagatatcaa cagtccgcaa agatggaagg
    15241 cctcatgtgg ttcctatttg gttcattctt gataagaatg attccaatat aaaggtcgtg
    15301 ttcactactg gtcaggactc attgaaagct aaacacaagt tgagagatcc gcgagtaagt
    15361 ctttgtgtag acgatcaaac cccctcgttt tcatttgttt taatagacgg aatagcggat
    15421 ataaacaaag aaccagatct acgtgagatc ctaaaatggg caaccaaaat agcaggacgg
    15481 tatatgggac aagataatgc agaagcttac gggaagagga atgcggtaaa gggtgaattt
    15541 cttgttagga taagaccaac taagattata gcacagaaag atatggtagg gcagtagcac
    15601 aaatttcgta gaccgtacgt gattttagcc gttcttgtca agatagcgga attattatgc
    15661 actactactt gatctttcta acacaatttt aaaaccaaac gcaaagtctt tgctactaag
    15721 atacaaatag agccggtaca gcacatgtct gaaaatcgtt tgcttgtgga aaaattataa
    15781 gcctattctt tagtgtaacc tggccagtgt aacctggtca agaattgaag cccctcaaga
    15841 ctgaagtcct tacgtttatg tcatatttgc tgatccaaat gattacaagc cgggccccaa
    15901 aaagccgcaa aatccatttg gtctatatat gatggcattt actacgccag agcacttaat
    15961 gaagaatttg ccaaaagcca tgtcaaatat ttctaggggc atgggtgaag gctgcccgcc
    16021 aacgggctcc ccctacattt aaaatctgta tgagggagta cgaagacatg gccataaaag
    16081 tgggcgataa aattaccata aaaataaaga aatcagacag cagtggtggg atatagattc
    16141 cagacaaagt caccagaatc tttctccctt ctcatcttat tttttatact ttaatttttt
    16201 tatgtcacac cgtactatta aatatctatg ttcaaatcgc ttagatcaat ttacaactat
    16261 aatctgccgt gttatttgac gtaaaaaaga tcaataacta acgatcatta agtgattatg
    16321 agcaacatga caagaaaatg cggctgaaac gtttatctta cataaaagag acatttgtta
    16381 tacctccgat aaggcttctg tatttcacaa gacaaagtca aatcggattg tcaatatttt
    16441 taatcgatgt atcggtcctc tcctattttt ggaacgaggt ctccaccatc gtctgcataa
    16501 aaaccccata acagatttat cgtcagccct acatggatca gacaagaatc tgtgatcaat
    16561 gccacggtga cagctttaac gttgcgttat tatttccggc catagattaa gatccgcagc
    16621 tataatcgtg ccattcaatt cgaatatagt cgtttgataa gtatagccca tatttctttt
    16681 ctggtacaat ggatacttgt acatgaggaa aaaacagcaa aagagaaatt gacctaagct
    16741 cgatttctca taaataatta gtctgactag atatgaaaag tgaaatacta gtatagaaat
    16801 actactcttg aatgctgcaa attgcggaag agctactaag actaaattaa tgtatagcgc
    16861 atttatatcc tttaatcagc tgagagaata cttgtcgctc ttagtggaaa atggtttgat
    16921 acaatacgaa gaaggaacgc acacttacag aacaactgag aagggtatac gttttttgca
    16981 tatacaaaac aaaattgacg aagtagctcc gattcagcgg gaaataaagc tgaaataaaa
    17041 ttatcgtagc aagggtaatg accaatagat cactttttta tcatcagcaa aatagagaga
    17101 aggcaaagca gacagagctc caatctgaaa tcgtgcccac gctgctatga taacatgaca
    17161 ttaaattctg attttaacgg cttataccat ttttgtgaac agtgtgattt tagactatat
    17221 atacacaaga gaatagaatt caaatcttat aacagaaccg ctgactaaat ttattatatt
    17281 accaggttag tcgaaaaaaa ccactaatca agtattagag gattatgcca tgaccacaat
    17341 cctctcgatg catactcaaa tcatataagg atgatttcta aatggaatct atatgcgtga
    17401 tttgccatta ctaatattga ttgcttctat aatgggtttg tccattctaa cgacaaattg
    17461 gtatttcaat tccgtgatgg cggtaagcac tgggaacgga ggaggagcgt ccgtcgtcca
    17521 agaacaagta aataccgtca atcctatctt ggacaaagaa attaacaaat tctacagttg
    17581 tatttcgaaa acccaccagg acccgccaac catacaaata gtggacaatt gctattctca
    17641 aaattcaatt gcaggaatta gtggctccgg cagtatcaac caccactata acaataacaa
    17701 taacaataac aataacaata acacaccttc gaaaagcaat tcaacgattg ctacggcacc
    17761 tcccggtgtt ttggtagagg tcccttaatc cccctgtaca acgaccgcta tcaagtatta
    17821 ggtaagacaa ggtcgacaat agaaattatc gtcttttcaa attcttggcg cattaaaaat
    17881 tgacttttct tgaaaataaa gaggaaacct aaagccaggt taggccatgt gtatggctag
    17941 cgatttcaat cacgaaattc tatggcagat ggagtcgtca gagatggagt atccgcagtg
    18001 caatcttgct tcttatattg tacagctgca tcctatccta ctagtacttt ctcgtacacg
    18061 attgaatagc atgatgtcgt tcatctcaca aattagtttg atggagggaa tgatgacacg
    18121 acctgaccgc tggttttttg atcctgatct gtataagcat gatgtaatct tcaattatgc
    18181 gttgtagatt tatgacttct tttgcatttt cgccgccata ttttccaata atttcctgcg
    18241 tagacattct gacataatca caaaaatgcc ataattcact tttgtacagg cttagagtct
    18301 tatctgaacg gatagcgcct ttgaatagct cgtaggatct accagctaaa tttgtcccaa
    18361 tctaggcatc gacgttgtag tttgtatatt gtcaataata tcaggctttg acactgtgaa
    18421 taatgttgtt gctatagaaa tagccttctc gcatcaggct cgttcgcgaa ttgtttgttg
    18481 ttgctcaaac acgtccactt agcaggaact tggaaaggtt tgtgttggcg atacgataag
    18541 ttgacgtcaa tctctgcata cctaaaaatc aatggtaggc cgcgctcctt tttgatagtc
    18601 atacgctttc tccaaaccag ctgtatcgtt acttcttgat tttagcggaa ctgaatgtaa
    18661 caaaccagat atgtgctggg tgccagtagt ttttgtatgg tttagctata ttagcagata
    18721 aaaaaatgac taattgtgcc gtatttttaa gattctctaa atagaagcct atagtgtaga
    18781 gagatttttt atgtttatta gtcagacata taatatacta tctaattgtg gagctgaccc
    18841 ttgtcaccaa tgcctagtga aaagacaggc aaacatggtg tcgttttgga tgatatggat
    18901 actagtagta atcaaaatcc tgaagatttg aatcaattca atcctctgat cgagcctttg
    18961 ccacggtggt acgggtttta cagacgaatc aggttagttc cctggtggtt acgttataac
    19021 cgtggaacta ctgagcaggc aggtcttagg gacaacatat gtaagttgtc tacaagcatg
    19081 gggctagatg gtaaattagt aagcaagata attagacatg ctgtgtcaga attttcaaaa
    19141 catgggcttg gaataaatta ttatggttat cacaatatcg atcatgagct tgaggttgct
    19201 tactttacat tgttggcagc caatgggcag aagagacaag agcatagatt tagtaaaaaa
    19261 gatctcatca ctctgttcat agctgccctt tttcatgact atgatccctt gaaacaattt
    19321 gataaaccac atgaggattc tgttgaaagg ttcataagaa atgacggtaa aataaaacgc
    19381 tggatagatg attttgaagt aagcatagat attataattg cgttaatata ccacactgcc
    19441 tatcccttta aaggtatcat agcggagcat gcaaataaga gaatccaaaa attatttaca
    19501 acagctggga ttcctgagaa tgatattacg gtgcgaaaac actacgaaga tctaggatgg
    19561 tttctttcta tttcagaccg tattgcaggc tatagccttg gtaattttga tcattctaga
    19621 gaacttgcac gacgcaatgt acactcttta ggatggcatc cgtcactgat aaatgaagaa
    19681 tcggtcaagt attttagtgc attaaaagag gagaaagaaa tggttgagcg tgttttacat
    19741 ggcatcccaa atgaatacaa aaaaaatttt tttaacaatg tagaggcttt caaagaaagt
    19801 tgggaagagg agattaacat aagaaattca atcagaagaa aggaactgag tcttatatca
    19861 gtagtagaaa aaattgaagg cagccttgat tcatatatca cacgatctat actgagtata
    19921 tacaaagaat tacctgcgcc aattcgtcta aacgcagaga agtttatgag gtcactttct
    19981 gacagcaata caatcctaat caccttgaga atcgatgatg aagaagcgaa aatagctggt
    20041 tatgtgaaag gcggacaatt ggagaattac aagcttcgat ctggtacacg cgatgagaat
    20101 tgggggaaaa agaatactgc acatatggag tggatcagca ttaagcctgg gtattgggga
    20161 gagactggcg gtcaccttct taggttacat ttccttaaag aggcaaggcg tagaggttac
    20221 acatttgtaa ctagttatgt tcatcgtaac gtgatcgata acagaataaa cagaggtgaa
    20281 cgtataatga ttgtacaaaa atatgatcca gacaaactag attactatag agctgatatg
    20341 agcaattcag ttattcagta ccctggtcag agctgacaca ttaataagat tagagcactc
    20401 gatttaggtc ttattgtcca taaaaaaagc ttttggaatc atgatcgtgt ctagtttatg
    20461 attgcgtgtt gaactaattt tctgatacgt cttcgctatt tttaaaataa ccacagctta
    20521 tagatacacc cgaaatcaga ggtcgcacga tacggatcac tccggacggc gcttttctgt
    20581 tcgaatctaa gaagagttgt tagcaaggat agatgttaac aaaatggttg tagaaaagtc
    20641 caaacaatga tctgcgtggg tagtagaacg tgttttcttg tggtaaaagc agcctctaag
    20701 ttaaataaca tggtatattt aaaaaatgat ggcatgatgt cagaaaagag ggtgataaag
    20761 ataatgattt tgttttacag cagatttgga aacactgcaa aaatggctga agaaatagca
    20821 tacggcgcta aggaaatatc aggaattaat gttgccatga gaagaatcgc agatgacgtt
    20881 cctatggaag ttgtatccca gaacccagct tggagtaaag tagtcgaaga tctaaatgat
    20941 aggtatccta caacaccaat cgagaattta gtaaatgaac tgcctcagta tgccgcgata
    21001 atttttggtt ctcctactag gtttggcaat atggcagcac ccatgaaggc attgtgggac
    21061 ggaactagca gtttatgggt gaatggttct ctagtaggaa aagttggtgc agtttttacc
    21121 agtgcagcat ctgttcacgg cggtcaagag agtactgcaa tttccatgat gtttcctatg
    21181 ctgcaccatg gcatgataat agtgggtgtc ccatattctg taccagaact tacagagagc
    21241 ggcagcccat atggacctag cagaatagta ggaatgcttg ccaataaaga aatcgaagaa
    21301 gcagatatca aagtggcaag agcattggga agaagagtcg cagagatagc tgggaagctt
    21361 gtctgacaag taccgtctta gttcaaactt aacgcaccta agcaaggtca cgtagtatct
    21421 tgtacaaacc cagtctctgg attctacagc cattaccaaa caagggataa atccctgatt
    21481 gaatacagaa ccagtaatag tagttcgatc accaataata aaacattgta tgtgattgca
    21541 ctgatggtaa tcggaactct gattattaag ttactcatgt atcagatgaa ggtgaaagga
    21601 tctagaccaa agacccacaa tcattttgtt attgaaggtg atggtactcg tttactaccc
    21661 gattgctgtt ttgatctttt tatctacgtt aagagaaact tctattagca gatgccttac
    21721 gagatattag agtatccatc tgatgttact aaatcagaag gtttggtttc attgtagtag
    21781 caatatgcga aataaaaaag atgacttatt ttttgtgtta tgtgccccgg tgttgttcta
    21841 attctacttc agtcttaaat ttctttccgc aaacgataca tttgaatttc tttccgaata
    21901 aattgaactg cattactgat agattaaatt tgagatgaat atactaaccg gtaattatct
    21961 aggtaatttt ccaatacctg tttacacttg atcatccatt gaattctgtt tatgaggata
    22021 ttcttattga cctcgattag aatcaagatt ccagagacag tatttcctga cagggcaaaa
    22081 ccatccatat aaattttaaa cgattgttac agtgactgta ggcaactaac ggaagtcttt
    22141 ttcttctcag gcattgcagg gagagaaacc taaaatgata ggacgtgtta aatacagaag
    22201 acgtcttaga cctgtattca cgatgacata tattttaggt tcgcaccaaa acaatagcgt
    22261 tgttttggtt ccattcgaat tgacttgtcc tgcaggacca aaacacgact cttcaatctt
    22321 cgggatatat ggaggaagtc ttatatgcta aatgaatgtc ccgatagaga tatcaaaatg
    22381 aaacacaaac aagaacgtac atgtgccgta ccagaatgca aaggtaaccc tagctttgga
    22441 cctgtcctta tgcccaatca tagtttgacg ttcaaggaag catatttctg tattgaatgt
    22501 aaggagcttt tagtgcggga acttattgaa cgagtaattg cgaagaatat accacttgat
    22561 atgctgaata attaattaat aattgaaaaa caggctcaac caggctataa gccagaaaaa
    22621 aattatttaa tggacttcta aaaatattaa tataataaga cactcaatct acttgtagaa
    22681 agtatttgat ttcaagtatg cagaccgaag aaagaggctt gcattacact tctaccgctt
    22741 tttccccagg cagttccgca actaaagtga actcgagcga taatacgaaa agcaacatca
    22801 acttagaata ctaatggcaa ttataaagac gacaacaaat ctatattgta taactagtga
    22861 ccggctgaaa cggaaatggg aaacgctttg attcaacatc aagcaaaata gagaacatga
    22921 atttcacaat taccaacact gacaaaaaac caataactag tgtattttgt tgacttacca
    22981 tggtgcacaa aatagcagtt ctagtcccag ttatgattac ggctattgca ttaggcgtgc
    23041 taggtcttgt gtttattcca ccaggagtca aaaatgcacc tgcaggcttt cctgtaggaa
    23101 cagttaggat aaatcatgat gtaattaagg ttgaggttgc aaagtctagt gcagaaaaag
    23161 aaagatggct tatgttcagg catgaaaaaa tgccatttaa ttccgcgatg attctagttt
    23221 atgacaagcc agatctgtat tcaatgtggc tcttaaatat agggtacaat cttgatctaa
    23281 tttggtttga cgaaaatgga aatgtagtat atatggtaaa agacgtacct ccatgtaaga
    23341 gcacctttga tatggctgac tgcacttata aaaatacaga acccgctaaa tatgtgattg
    23401 ctgcgacatc aggattcata gataaacatc gggttaataa agactcaagg atgagcataa
    23461 cttctatcta aattcagtaa taatattcga aactaaaaac caatataatc cactcctatg
    23521 tgatctgaaa tattcagtta accacagccg tgacgtagag atacccgttc gctgccagcg
    23581 agagattgtc cggtcataag agccgctggt cgtgcgtcgc cgtctcctat tctctgtctg
    23641 gacgtcgacg caagatcgcc ttgtgttcat aattggtcga gtgaatatta cccgccgcgt
    23701 ccgaatcaag gccgtccgat gcgccacccc tgtcgtcgtc gtcagcgtag atctttactg
    23761 ctcggaatat cgaaaactct tcttacatcc ttgctacgtg cgcccaaaga ctcgcgttat
    23821 tcaaagctga aggtgcaacg tcattggatt cttcagccat ttcttcttct ggtatcaaag
    23881 ttaactctac tttttcatac gaagatttat ttgataaaat gccaaaaatc tgatcttaat
    23941 aaacaaaaat ctgctaaagt catgattggg aacaacccat ataattagga cggcaaccgg
    24001 cttactcagc agattaaaaa gcaatgcccg tcatttattt taaattgata aaaagcagaa
    24061 taagtagtga cattacattg tcgatagact gtatttcata tgaatttatt tagttgtcta
    24121 tatcttaata ccttatcacc ttctctaaga aatttctcat aggacccagt ctgagtaagt
    24181 agtaataaaa aaacatctct tttagtattg gctcgccgaa tttattacgg tattatacgt
    24241 cctttattac gtacgatacg gtaacatatt taatggctca tggacgtccg cttttgcaat
    24301 atttctgctc agagtgtagc acacttctga cggagacaga caagtctatt gattttatac
    24361 gatcgtctga agaatgccca aaatgtggtt gtctactttc agagagtcta aaaagagcgc
    24421 tgacaccaca acaagataat agtaattcaa cagcatcact attatttcca ccaaaattcc
    24481 atactgcttc tagattaaga tttgatattg aaaaaataga ttctattttg aaacttggaa
    24541 ttggcgaatg catatgctta gtcggaaacc attcaaaaat tcttatcgaa agactgtgta
    24601 ttagcgcttt actaccagag agccatggtg gatttggttc tcctaacgta ataattattg
    24661 atgctggaaa cagttcagat atttatagtt gtgtgaattt cgcgcgccaa tatggacttg
    24721 atatcaaaga tatcttgaag agaataattg taagtaggcc tttcacaatt tatcaattag
    24781 caaatttagt cacttatgaa ttaccaaacg ttgttcgtaa attcgatacc aagatcatta
    24841 ttatttcaga tatacttcgc atgttcttag aagacccgca agtgagagtt ggtgaaagta
    24901 ggcgattggt aaaagaaatt acaacttcaa tacgacaact ctcaaccaat acatcagtta
    24961 ttgtaaactt acgctgtgat cagccttctg agtactctca aatgctttta ccaagtattg
    25021 ataaatgtat acaaattaca aacggcgaat ccgacaaccg cctttttgtc caagtaaaca
    25081 atcgaaatac tagccacaaa aaagaagatt gcaaaatcat acaactgcaa cgacgagatc
    25141 tggaaataat tcctcagaga taatactaca actaattctt tgctaatgaa acaactatta
    25201 gtggatgagt cggatgtttc tatggataga gaccaggcaa tcagtggata aaaacaatag
    25261 atggccacac tctcatttat tgactcattt attga
//
LOCUS       DAOR01000093           12269 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_41467, whole genome shotgun sequence.
ACCESSION   DAOR01000093 DAOR01000000
VERSION     DAOR01000093.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12269)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_41467"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3961 a         2367 c         2265 g         3676 t
ORIGIN      
        1 cttctgacgt ttgcctactt caggaattgg gaactcttcg accgatatca aagtcttttc
       61 catgcatggt cttgatatat ctattactgc tagcgtcatt tattcttgct cccgtagata
      121 ttttcttaaa agtcctttaa aacgtctagt tccacttgtt ttgattaatt ccctatgaac
      181 tcggctttaa aagatgttca gaatgtgaca tttatattaa atttgcaggt ccaagatgtc
      241 cctgttgcag ataccctctg agatcaaaga agaggaatta taccaaggtt agtacgtcta
      301 gttccaacac agctgttgtt ccatgagttt gcttttccta ataacatgct cgattatcgc
      361 aattaaagta cagaatgcta gaaatagctc atatcaaata atatcagctc aaatcctcta
      421 tctatgctca aagtatacag attatgatca tcacatcgag aatcagcttt cccattgaca
      481 gtagacaaat attgttttaa cataagcaga atagtaaata atttctgtat gatccacagt
      541 tcttcaattg tctattgttt attcttggaa tccttactgt aaaatggacc ttctttcaag
      601 gcatattatt aaattcaaaa gactttgtaa tgctagcact ccccattttc tgaatggtaa
      661 tggaatgtct aattagctcg aatataaatg taatattaag ttctagatca atattctaat
      721 agatctgcat attgaagctt tattatccac gcaatgtgaa gagacgtttc ttcgagcttg
      781 acatctcaat ccaggagtat actctatgac gagatagcct aataagttca aattgcaatg
      841 tatattgtag tatatctcgc caatctcctg atgtctcaac agtagggacg aaataacata
      901 gatacatatt cttacgctat ctgcttttgt catgggatga ttacgcagta gaaatacaaa
      961 aggagaatga aataataaaa tggtctgtaa aggaatattt ctacgtcata aggcgatgaa
     1021 gccagttggg actggtcgtt attctattgg acagaaacgt tgtcaagtgt gtgagatctt
     1081 cttaacatgg gaaggattat ggtgtccatg ttgtggatat agattaagaa ctaggccacg
     1141 aaacatgaaa tataaagcta aattgcaagc acggaagaaa atagaaagag ctagatcaat
     1201 agcagtatac gaacggccgc ctattcaagc tcgtcaaaaa catattgtac acaaacggca
     1261 tctttgtata aacgtatgat ataattcggt agattgtcat ccaatgaatc gattttcttt
     1321 gctagttgaa atgaatcgat gtttgatttg atattagaag tttttaaccg aattgattgg
     1381 ccttttttca tcgcagttgg atgagacaag ggtctttcta taatggaaac agctccacct
     1441 taaaaaaatc ttgttatcaa cccacgagta tggcgccggg agtgggactt gaacccacga
     1501 gtcccgaagg acatcagctg gcacgtccat gtattcatat tgatctcgag gctgacccct
     1561 tttagggcca tagacttacc tagctcgggt atcccggcaa atatccttga taaggcatta
     1621 caatataatg ttttttcatc gaatctcaca taatacatac aaatcgattc tacaaatgtt
     1681 acatagctca gtatgagagt tgtgaatcat tctttcacaa gctcaaaaaa tgcggaggtc
     1741 aagcgcgagt tggcgctagg cgaattcatt atggaacttg aaaatgggga ggtcgcccca
     1801 tttgtatgtc cggatgcatc ggctatcagc tttcaactac ccgatataca cggatttttt
     1861 tcgcacgaca actccttgtt aagatagttt caacatcatt actacataat acacttagaa
     1921 tatgaacaga aattgttcac actacatttt gctgacaaca aaatgtaggt attgctccca
     1981 tataacatcg aaccccaatc ctttgcttaa ttattcttag cattcttatc ataaaaagtg
     2041 ccaaagataa taattatcta ggttataatt tgatcactag atccagatga atgcatcatg
     2101 attaaaatgc agcaataatt tcgacaagtc aaatatattc gataagagta aagcaattga
     2161 gaaggctacg acccacaccc cattgtcaat gactattatc atcgccacta tggtaatgat
     2221 agccggagcg ctagctttta tgtcaattca aaaatcaaat gcgcagctga aggtaattca
     2281 gagcgaacaa caacaaaagc ttggccagct gaatgattgt ggaaacaatg cgacctgcac
     2341 aaatataggc tcactcagtt ttatgatatc tagccaggct gctaatgata caaagctgaa
     2401 ttacatggtc agcgaagcaa attctattaa agactatgtg caaaaagata cgacgccttt
     2461 cattttgcct tttccatgag aaactttatt tatgacttac agtctggaat gccattatta
     2521 gtagaaaacc aatttttagg taactttgcg gaatgacctc tgtttggggg ttttgaactc
     2581 ggactaatct accaagccac agtcctttga aaagcacgct ctccaactat tacgagcggc
     2641 cacaatatgt gatcagctgc agaaactgga aagccttcgt tattcattgc ttcgagtttc
     2701 ataattgata aacaacatag atcatgattt tcgtgctcat gtttgcaaga gaatagttcc
     2761 ctagagctag tctagtgagt ttaattaata cacacactca ttccctatta atttccttag
     2821 aaagtctgac aggctgctta tagcgatgaa atatgattga catgcgaatt ggttgtatgc
     2881 ggataggttt agtgacatat caccttagcc taccttctat aacgaacata agctcgacaa
     2941 aaatgctacc tcctaggaca tatcttttct ctgtaagagt atcacgacta taccaactac
     3001 aatcgataga agggctggta tatgatgttc accagtcaga tacaatatgg acacggtaat
     3061 tccaattagc agtacaacaa ttcctccgct tacttttttc aacggtatta ccatttttca
     3121 cttttcacct ttcccctgtt acgtctttca tatcacgttc tccatacgac ctactggtaa
     3181 tctgctctaa actaagcaac aactattatc gtaaattgtt taattctcct ttttgaaggt
     3241 gcacacggta aatctatcta cagtactagc aaaagccaca atgcggatag atcggtctat
     3301 caaacgtcgg ataaaattcg gagtagcagt taaaacattt catttttcca aaagtcaatc
     3361 gagttctaat cggagctatt agtgcactaa cacagtcgtc aggatttatg cctatcagaa
     3421 aactcgtctc ctcctgattt tcctttgtaa ccgttctgct cccctccgtc tgcctaagat
     3481 atttaataag cataaaagat tttgctatga aagcaagagt aattgaaacc atgccaccaa
     3541 caagagtcct tatgtgatga tcttaaagcc ttcaccccct tctttgggtg aaacaataat
     3601 ggtccctatc cggatacaga ttacaccata agctacaact ccaaggcgct aacttcccta
     3661 tacaatgtaa catggtgaca gctattacga ttcatctact acgccaagac tggtgtttgt
     3721 ccgacatcaa tagaaatttg ctttcgcttt cacgtataga taaataccaa gaccatcata
     3781 gtatctctta cgataaagcc atatagtagt acgttcacaa tcaattcgag ttatgcagga
     3841 atcagcataa tattattaca taatccaatt cagtttaaat aatgatgatg ggcatatcat
     3901 tctgtgctag ctgcgtctct taatccgccg acacgcgaag aagttaagaa attaactatg
     3961 attgatattg ccataatggg tgggttagcg gattctctaa gaagtactct caaaaaatat
     4021 attcaaattg acccatatac catgtcggat ccatttggat ctgaagatga gtataactat
     4081 tctgtcattt tagataggca agaaccacga agagcagttg ctatgatcaa agatgagtat
     4141 aactattctg tcattttaga taggcaagaa ccacgaagag tggttgctat gatcgcttct
     4201 aagttagaac cacttccgca gctcccatgg gacgatatat tgggtgatcg attggttaga
     4261 tcatcattgt caaaaccgga tgctaatgcc ctgaaaaatg aaatgatgcc caaggatact
     4321 aacaactttt accaatacag gcgttctggc aagattactg gcttcataat gtttgcgttt
     4381 cagatctgtg gacagcattg aacattagtg cagactttaa caattcgttc gcagcatttg
     4441 catttttggg ccttcaaatg ttctattttt aaaaatgtca tcaatctatc agaatatttt
     4501 gccgcaattt tggccgacca tgccttttcg cccatcctgt tatagtaagc cctgtgtccc
     4561 ttcgcttgcc cttaataaaa atcagtagta gactctgaag ttgggaaaag gtaaaggccg
     4621 tgaacagatg atttagtcat acataactca taatgagcat gccaagctcg atatttttaa
     4681 ggaatttcga gttgtaactc gtaaaataat aatcatagca taggaatcct gtcttcgctt
     4741 atgcaagagt cattaagtag aagaatccta ataccaaatc gctaccttac tgtgtaaaat
     4801 aagtacaagt aatttgcaaa aatttacatt gacggctctt ctcttatctt caaatttcca
     4861 tcggtcaaaa tatctattcc tataatatcg agaatttctt ttgctcgttc ttttccctcc
     4921 ctctcgacaa tatttctggc cattttcaag cggtaagctg gcgcccagtg ctgacctatt
     4981 ttatatttgc ctcgcatccc ctctgggact atcttgatta cagtgacttc atggacagag
     5041 ggcagataat gatcaagtga ttcatatttt ccctcttttt gatacttttc cattagcgaa
     5101 tttagcgcgt ctgccttttc tttgttatcc tctacgatca ttgcgttacc tttgataact
     5161 acgctaatgt agagagtatc agcttgcgaa gcgtcaccgg gatgaaaata gtaggacggt
     5221 aagaagcaga cgtgtcgatc aacttcgaac ccaaccttcg aatttccttt tatattgtct
     5281 agtttttcac cgaaaggatg agaatgcata taaactgcgt ctagttttat tattctacgc
     5341 gaacggatta gtgggtcatt tgcttgtacg tatacaaaat tcattggtat gacttgcgga
     5401 tagccattgg cgtctataga agctattcta ccaactggct cgttgtttaa aaattcaata
     5461 atcttatgct tggattgtat ctccagtttc tttacgagtt gcattgctcc accatcatat
     5521 ttctcacaaa aagaatataa ttaattaact tattcaagaa gcaaagcgat cgcaaatttt
     5581 cataccaaag acgactccat tcttatttct tcatactgag cgggcatcaa ggatttgaat
     5641 tatagaacga caaagcaatt ccacacaaac gggttggcag ctaatcaggt aaattcaaat
     5701 atgccgtttt tctgagcttt gctgtcaagc taggaatcga acagagcgat gctgcgataa
     5761 atataggagc cttgggagct acaactctat tgtttaatca tgttaaattt gcgatgcccg
     5821 gtattactac aaccaatgat agtacaggtg attccctcaa aaatcctaag attctaactc
     5881 gaaactaatg cagcgcatct gcatatttgt agtatatgtt caaagatcgc acctcactat
     5941 ttggtacgac agtaagtgcg aacccggtca gggttagaaa ctgactaaga taggattgaa
     6001 actttgtttt tgataccgta cttgtggctc gagccttgcc agtcaggcaa ttattgacat
     6061 acggtactag tactattaat catgtctagc agttgcctac aattatgcta gcatcataga
     6121 acaaagtgtg gtaatcccga aatgaaaggg aacatttatt gaatacatta ggtaacgtga
     6181 ccagttttct gcaagttcat tgcctgggag caaccaaatt tatatagttt gttcttgtac
     6241 gctagattgc ttacagggcg gtaccctccc gggagggcgg caaccgatat tttcgtacat
     6301 ttttacacaa acttttaaat agaaatgatt tctatgagcg tcagatcgcc cattttagat
     6361 gttatagaac aatgtacaga aagttgtaat ttgacctccc tggaggatgt gggcaggaaa
     6421 aaaatgacgt aataaactta atatcgtaag taccaattat tgagtttatc cctaaatgag
     6481 taataacgtt ggcatagatt ttactgaatt agcaccaccc gatggctgcc aaccaacaca
     6541 agtcccctct aaggggcagg atgtagaaaa acatgaagag ctgataacgc taggatttcg
     6601 ggtcaaactg aaggaaaagg caatcattca ggactaagtc aataggatgt actaccaatt
     6661 cgtagtagat cccaatacaa acgaaccaaa gagaatgttg acgagggtat cggaaattgg
     6721 caggtagcat caaaaactaa gtggaaactc aaatttaacg ctcatgtcct ctcaatagct
     6781 caatttccgc tcaatcaact gttagattcg gcagcatttg tgtaaatgcc cgctcgtcag
     6841 gggagaaaat aacaatcttt gtcaatctgt acgccaatac agaccataac ccatcaacca
     6901 ccaattcaat ttccatgaca gggaatactt cgaaatctgt tgctttgttt tcctttagta
     6961 cttctgctag tttgacaggg tctttcaata gcaatcaaat tacgggcttt caatgaaagg
     7021 aatccatgga gatgattggg aataaacaac ccataccgga atttcataaa acattaaaat
     7081 agtattatcg tctaattaat ttatatatgc ctctcctaat ggttaaatgc aaaaattgtg
     7141 gattcgaata tcccttcgcc acacaaatga atgcggagac actcaagact attagattgg
     7201 acaacagtaa ggaaaactgt cctgaatgca aacgaatctc tgcatataat aaggccgact
     7261 acttctttaa ataagccaca ctattttttg caggctgact aacgtcacta taggtagcaa
     7321 aaggatgttg aggagattcg actgctatgt ttaccattgc ttgatttatt tcagattatt
     7381 atgaaatcaa ttcgccttaa acgtattgtt atgtttacta gatgatcctt gtcgttggaa
     7441 tatgcactag gaaaaaagga cagaggaata cgttctgcta tcattgatcg ttaaagattt
     7501 ggctaatgtt attactgacc tcacagaaat tagtactttc ggggaaacat ctcctcgtag
     7561 ggctctagaa ttcttttgca aaattccttg cccttttctg taatgttata tttgattatc
     7621 tttttgttgc cccatggttc ttctaccctt ctgataaacc ctttttgttc catgtcattg
     7681 agtatgtctt tatgctttac tagattaagg ccacaatagc ttaatagaga agtttggtta
     7741 agctgaccat actccgttaa cttgagtaaa atatcttttc taatgtaaat gcgatcacga
     7801 tattcatgcg acaagtattt gatttgactg taaatggaaa ggaaggcacg aagcattaag
     7861 ttttgttatg gatcaagtag tatccactaa acatgtccag aaaccagtag tgattttatt
     7921 tctcgatctg tccggctcat tattcagaac gttaatcgat gacttgatcg actactggcg
     7981 cacctaggtg aaatagactg ttcataagaa ataaaaaaag aacatcataa tgcttccacc
     8041 atttggtaca atgaacaact tcatactttt ttgagtttta tcttccctgt tatgctctac
     8101 aagattgcat gacttcccct ttttgtatat cattctttta atttcggacc aaggcatttt
     8161 aaaaaacatt ataatacctg ctaacagtgg gtgaagattg aatacgcagc gtcatgacgt
     8221 cttcgaagaa aaggctgata tgttgtaatt cttccattgt tatgagtctg tttgattatt
     8281 actacagcag tttgaattct tcatgtaaac cgcagcttac tggcttttaa cggaatttaa
     8341 caccatttaa taccaatgat aagtatgtca acaaaaagag aacccgagag agcagccaca
     8401 acatttgctg aagatgataa gacaatagag gtaagaacag cattaactga gcaacaccat
     8461 tcagtcgaaa aagcattaga tgaaaccaaa gataatatca aaagaacaat agaggaagca
     8521 agaacagaca tcccacgtaa tacccaagca ctcaatgatt atcaagagca ctctctccag
     8581 gcaactaaag aaattacaga tagttacctt gaatctcaaa aggagatcat aaagtcattt
     8641 cagtcaacat gggtcccata cgtagagaat gcgtatggca cattttggaa taattgggca
     8701 tctccacgaa gggcagctga aatctatgca agaacagtaa gcacttttgc agataacgta
     8761 attgctacaa cgagaatatc tagcaatgcg atgtttgcaa atatggaaac attctagaca
     8821 tttattcagc gtgagaaaga tgatttaaag gaattttcga gaataggagt caatgccgca
     8881 aaaacgtttg cacaaacctc aagagatgtt tcgagagaac atgatcacaa taggtactac
     8941 taaagtacct tttcgttatc acacacaatc tagccaattt cacctctact aattctcaat
     9001 tattaaacta tagtatgaaa gtataatgct tttttcgatt aatcttatga attatcgcag
     9061 atttgttttg caaatttggt tctttattgt ggctgcaaca tgctttgcgg aaggcagagc
     9121 tagctggtgt tctagcaggt aaccgtgtta ccatgtagat attcttatgg actcgtgaga
     9181 atattcttaa atgagtaaat gtatatggat taatctataa tatttacaac catcatggct
     9241 ggtaaagtat atcttgtatc atccagcaat atcgtgtatg aaaacccagg atatcgaaac
     9301 atatgggaat ttattgtctt ttgtgtaatc atccatgcta gtatgtgatt attgttttgt
     9361 ttgtcggagg atgatcaaca agaagatatt gactataaac gaccaaactc gtcactcacc
     9421 cgcctaatgt tcaaggaatc tgatcattca cgcttctttg aattcacttc ggtttacacc
     9481 ctgttggtaa ccttttatct tatctacttt tattcagcag atgtaccatc ctccgacacg
     9541 aaattaaata accagcgcaa ttaatatcca atcaaagaat ctaatgatag ttaaaagcca
     9601 aaaagtaaag agagccctcc ttacagcttt ggctgatgac gagcttgtgg aaatcatggg
     9661 ttctgttatg catcactcaa aatcctttaa tgatattagt agggataatg gcaatattcc
     9721 acgtactact gcttttagaa aaataaagtg gcttctcaac gaaggccttc tcatagtcga
     9781 caaaattgtg attacgccat ccggaaagaa atttagtttg taccatagta cattaaaagc
     9841 tatcaacgtt agatatgaga ataacgatgt gattgtagaa gcagagcaaa attttgacat
     9901 cgcaaaaaag tggctagaaa aatttttctc gtttgattaa ttacaggaag gatataacgg
     9961 aatttgtctt tatccaatac ccacattaaa gggtctcaat ctttctgttg gctatctagt
    10021 gcggttcttt cactacgacc gtcaattcca cgcatgaagc cataataaca gtaacaatct
    10081 gaattagatt taacttggaa catcaaattt attgaactca agtgccccaa tccaatttat
    10141 aattatttgg attctcaatt caatttataa taatcaagtg atagctcaga tgctcagaca
    10201 ctgctccatt tccagttatt tagagacaac gagattagag gtgtacaaga acggaggaga
    10261 tgcgaggatt aaacacgcat gtcatcgatt ctttatcagt accaatttaa tatccaaaag
    10321 atatgattag tttgctctgt ttcctatcgt ataagtctca taaattaaac tacagactcc
    10381 ggcaatagat caagatcttc atctaaaata accaccacca ttaaactgta gctataaaca
    10441 cctgcatcat caaaataaac gaaatgatgc taccgcctgc gttggctgcc gactgttatc
    10501 atctaattca aaaattcgac aaggttataa aaaagagcgt acatggattg aatacactga
    10561 taaacctcaa ccgtatatat caagaagatt gtctgagctt tatgagtaag cttagggatc
    10621 aaaaaatcct agtagatgtc gttgttactt ctccacctta taatatgaac aaggaatatg
    10681 gaatatacaa agacaataaa gaaagggaga agtatttaca atggatgaag aatgttgcag
    10741 aaaaaagtta cgatatttta aaggacgacg gctcgttctt cctcaacgtt ggaggtagac
    10801 cagtagatcc aacagctcca ttcgaagttg ccactcaatt tagaactatt tatgaactcc
    10861 aaaatacaat tcattggata aagtctgttt ctattgacgc agaggacgta ggtaagaata
    10921 atgagttccg tgactatggc aacatatcta ttggtcactt taagccgata gttagtgatc
    10981 gatacctaag cgatcttcaa gaatatatct ttcactttac caaaactgga aatataaaac
    11041 tggataaatt gagaataggc gtaacatacc aagataaaac taatatcgga aggtggaaat
    11101 ctgcaaagca ggataaacga gataggggaa acgtatggct cataccatat ccaactattc
    11161 aggaagggcg cccacaccct gcagtatttc ccataaagtt gcccgaacgt tgcataaagc
    11221 tacatggaat caagccaaac atggtagttt atgatccgtt catgggaata ggcaatactg
    11281 ctctagcatg tcttcgttta ggccttaatt atctcgggac tgagatagac gctgagtata
    11341 tcaaagttgc tcaagacaag atagcttcaa ctcctttaga gacctatctg tcggaggaca
    11401 atcatgaatt tatttaagaa tttgcagtta gacttagttc ttgtacttgg atgtaaattt
    11461 ctcgcaattg tttgcgagcc caccaaaaca ataaaacaaa tgcagacatt catatacttg
    11521 aatctgtgtg tcactaacat atcttaacac tttaccctcg ttacaataat gcaaaattca
    11581 actatatgct caatgtgcaa ggaagatcag actattatca cagacctatt gactggtgag
    11641 atagtttgta gtaaatgtgg tctagtaatt tctgataaaa ttcaagacac cagacaagaa
    11701 tggcgtgatt tcgtgaattc tgaagaagca aaagatagaa ggaggaccgg aatgcctacc
    11761 tcgttggctc gtcatgacat gggactatat acaatgattg gaagaggccg taaagatgct
    11821 agagggcagc tgttggatgc aactatgcgt tctacaatgg gaaggttgag gacatgggat
    11881 cttagaaccc aagctagcgc taatagaaat ctgaggactg cctttaatca acttaacata
    11941 ttgaaggata aacttggact tcccgatgca ataattgaga aaactgcgta catctacaga
    12001 aaagcccatg aaagaggatt ggtacgtgga aggcagacag cagcagcatt agccgcggct
    12061 ctctacctag catgcaggca agaaggaact ccaagaacgc ttaaggagat ttgtataata
    12121 agtaacctta aacgcaaagc aatttcacgg gagtataggg atatggtaat tgaacttgac
    12181 atcagaattc ctacggtcga tccaacaaag tgcattgcta ggatcgcgaa ccagattaac
    12241 ctgaaccaga agataaagca gaaagcaat
//
LOCUS       DAOR01000094            5273 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_16683, whole genome shotgun sequence.
ACCESSION   DAOR01000094 DAOR01000000
VERSION     DAOR01000094.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_16683"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2909..3208
                     /estimated_length=300
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4788..5148
                     /estimated_length=361
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1397 a          832 c          933 g         1450 t
ORIGIN      
        1 tattcttgaa tagtactgct ttctagtaag tttcagttga gctataagaa tatcgcactt
       61 cgagcgtgat gaggcgatat gctacgtaac agatgctaaa tccaagagtt gaatagacgt
      121 ttcgattctc gagcatacat gttggtttat caagactgat ataaagatca gtacaaaaga
      181 cattaacata taaccaatta ttatatcaat catttagtat ttgttaaata ttataatcga
      241 ttcaggaggg caatcaatca gatgtaaact gtgcaacaga tgcgcatgaa gtgacaacaa
      301 acagtcaaca agtcagttga atcacaatta cgactatatc tgccgtctaa tgttctaaat
      361 gaagagaagt aggaaatgct gagaaatctc cttgaatctg agattcctga ggagatggtt
      421 gcggagcagc ttgatcttga tgtgcaattc aggaaagaag aatgtaacat tgccagttct
      481 ttgtaggtca tacaaaaata ataaatgata cacctaatag aaggataatc gtcctctcta
      541 tgatataccc agctattgct ttggttggtg taataagaat ggctactgca tgcatcatac
      601 atacttcttt tgcatgcttc tagatctctt ttgtaaccaa tgtgactgtt gatacgatat
      661 tgtttagtct tatgtcagat ttaagagctt gttgtagctc ttctaccgtg tcagcagcaa
      721 ctcttacaat aatatcataa gcgccatata cttgataagc ctgaataact gccggtattt
      781 cccttagtct gctggtaagc accttatcgg atcctaaggt acagttgatt agaacataag
      841 ctgatggcat gtctatgtac tatttattta tcctcaatac tgaatcattt tctgaataat
      901 aaagctatgt gtatggtccg cggattgcga atgtgaacat tggaacgtaa ggaacgtgtc
      961 atctgctaat ttaattagcc atgctttaaa tggaatacct agtaatgcag atttaccaga
     1021 gatctgtagc aagagatatt ggcagctgac ggagatgtgt cagtaatctt ttttaatgtt
     1081 ccaatataca gcactaacag ctctttgata ggcatacggg gagcagctta gatttgcatg
     1141 tttttaataa atcgctttaa gcattaaact tgactcataa tgagcgtatc ctgtaacttg
     1201 accaacatgg tcagaaggta tctgattcag ataaacaatt atctaataaa atagagtcat
     1261 ttgggctcag tagaaaccat ggaatattat aataagattg gtcaatctgt ggatgttgta
     1321 tgctatacat ctgaatgata attctctgtt ctgtgttttc actagtcttg atgtaatatg
     1381 ttctccaaat agtcgtttta tcactgaaac tatggtttca ttcttttttc gttgactgta
     1441 caacaatttg gaatacccac gcttcatttg tttcctgtat ctcccatgtg tctttcgtat
     1501 tggcacatgt tcatatcttg ctggtataac gctaagtgca tggagtacat ctcttactag
     1561 tacatgattg tcttcgctat catatgcttt gtcggctgta acaacggata gcggtactaa
     1621 ttctgatatc tttgttatga gtggtcggaa atctatgcta tcatgtctag tcggagcacg
     1681 tctgatttta attgtacaga ttatctgttg aagtacgtca gctgctaatg atagtttaat
     1741 gtattttctt cttagtttta ctctttctgc gtagtactga gaagcatggg ttgctttgaa
     1801 tccagacgaa tctatcccaa cgaatagttg tcctattttt gtaagaatta tgaatgatga
     1861 aattatcttt tggagtagag ttccactgat tctttccgtg aatttctgaa gagtagtgaa
     1921 atgtggcaca tgtgataact ggagaaatgt tctaaggtaa tacgcttcaa ctagccattc
     1981 cgaaaacata cgataactct ttccttcgta ctggcgtatc actaataata ccatatgctg
     2041 ccagactgtg aatatgtgat tgctctttct gcatagaaat aacggtattc tcgcatttct
     2101 taaaacacgc agcatggtat ttgctaatct gacatacctt gactctctca ataggtatat
     2161 tgtattatgg tcttacaaag ctttgacggg attatcagaa aaaaggctgg tttctacaaa
     2221 gccagaggga tcaactcgct gcagagaata ctatatagtg gatcaatgaa atcttctaac
     2281 actaattatt aaggtgcgga tttccgatat tactaataat attgatttgt ccacgatagc
     2341 attgttacta tcttcttaaa gcttctagtg gagatagacg cgacgctttc caagcaggat
     2401 agactcctgc tactaggctt atggatagtg agagaagcca gacataaaga aggtcatttg
     2461 gaagaaagat agggctaata tgaggagatg ctgctccagg tggtcctccc cctgaagcac
     2521 cgaaaccaga tgttaataga tatgcaacac cagtaccagt aaatattccc agagttgatc
     2581 cgataagacc aatgagtacg gcctcactca aaaacataga taggataaac cagggttttg
     2641 cacctatcgc tttcatagtt ccaatttcct ttattctttc atttacagac gtgtaaagag
     2701 tcgtaacaat tccaatagca ccaacgagca atgcaatgaa cgcaatctcc aaggtaaatg
     2761 agctacttcc actctgagcg tgcgcctgtg cttgcatgat tgctttagga gtgatgactc
     2821 caatgttgtt actaccgtat agactagtaa tttcttgttg tacagcatta acataagtac
     2881 cagaaagtgc aagtacaacc atttcgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tgaataaaga gttgactttg tcacctactg
     3241 atgtaactga gttcctgtta agaaatcctg ttgaacactg cctaggctaa agaatcggtc
     3301 taaatctatg caaaatgcat ttatatttgc aaaagaacgc agcataatgt ctaccaaaaa
     3361 attcactgat ttggatctcg ccaagggggc ttctaactac atgtattatg ctaatattcc
     3421 aacgttcttg aaatgccaac atgatgttcc acaaaatgct gatattggaa tagtaggtgt
     3481 tccgtatagc gggggtaatt gggtagaaag gacacaatat cttgcaccca gagcagtaag
     3541 agatttttcg atgggttttc atagaagcca tcgtgcattc aaaattaacc cttttgaact
     3601 atgtagaatt tgtgatcttg gtgacgctaa tataagtaac gtccttgatc caaataaggc
     3661 tgtaattgac atataaaagt tttacgaagt gatagatcag tcaggaaccg tacctgtttc
     3721 tataggagga gatcattcta taaccttacc aattcttcgt gcaatagccg gagtcaattc
     3781 tcgtcataag ggtccagttg gcgtgattca cttagatgct catgttgacg catatggacc
     3841 agtggcaggt attaaggagc attgtggatc gatgtttatg ctcgcattag aagagggtct
     3901 aatagatgcg actaggacaa tccagatagg tattagagga gcaatgggag atttgcgtca
     3961 agacgattgg gcacatgata caggatatcg tataatcgag attgacgaat tcaatgatat
     4021 cggagtagac tcggtgattg cagagacacg tcgcatagca ggtgatggta aaccaaccta
     4081 tatcagttgg gatttagatg ttctagatcc tgcatatgct cctgcagttg cagatcctga
     4141 aactggcgga atttcaatta acgaagccct tagatttata cgcggacttc gtggtatcaa
     4201 cattataggt gcagatattg tttgtttcac tcctcatctt gatactacaa gaatcactgc
     4261 attgaatgca tcatctgtac tacatgagat agtaactata atagcagatc ggatttccaa
     4321 gaataatccc aaaaaatagt ccctgctact aaaagaatgg aagattattg cttgaaaaag
     4381 aatctgactg ctgctcttca gcatattcac tgattgtaat tcaaagaagt gtagaccaaa
     4441 cttcggagtg acttggtgga gatggaattt tgtctacaca cttagcccct tgggacaact
     4501 acagcctatt ccttgtggcc tgccacaagg tggacaattt ccttcgatgt ccaggtaata
     4561 atgttcacag gtaggacaca gtttagtagt catgtgtatt atccatgatc ataaatataa
     4621 aagatttttg taacctactc cctgttatga taattaaacg gggtacaaga taaggatcct
     4681 cctggtaaat caggtattct tggcacgact aatgtctccc ttgttttgat cttatgtcac
     4741 gtcggggtac ctatggtgta aaccgtctgt tagggtattt ctcagacnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gacaaaacta
     5161 cgtcagtttg gatatgggtt gtattatcag aatgtttcaa tcaatgatat actaaactgg
     5221 actgaacgtc cgtttgttat aacaatattc aaggagagtt atttacccgt aat
//
LOCUS       DAOR01000095            4458 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_39576, whole genome shotgun sequence.
ACCESSION   DAOR01000095 DAOR01000000
VERSION     DAOR01000095.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_39576"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    536..749
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3943..4182
                     /estimated_length=240
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1259 a          762 c          679 g         1304 t
ORIGIN      
        1 gccatctctg gatttagtag cttggcactt gctcttctat acataaaaaa gcttgtaaag
       61 aacattaaca ggccttctaa atctaaagaa aacatcgcaa ttccagtatt gttatacgta
      121 attataatgg gtagcgcata tcttttgctc ccaccaaatc ctgatagaat tacaataccg
      181 atagatttag ttacaaattt tagaattgca agtgcattta ctattggcat attttggggt
      241 ctaatgggca taattctagg ctcattctgg gacatactca aaccacatga aacgagcaaa
      301 attgcatcga tatagttcaa gaactcttca atcacgacca ataggacaaa aaattaaaca
      361 tccgtattgc ggagtagctt tattttttat acgagccaca tttaacgacc agattatagc
      421 taggttttaa actaattatc tatgcagata tgcaattacg acggttgcag ctacagccgc
      481 aggaattcca aatatgagag catactcgac gtaaacaatc gttgaataac ttactnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctgatgattt cacggcacca acattttcaa
      781 ttctgatggt tattctatat ggaccgcctg tcttttgtgt taacgcctag gagtggtcgg
      841 ctccttgatt cattgccctt gcctcaaaac ctaagttcgc ttacttgaat gtgatttctg
      901 taatcacaat agtcgcggtg taaacatata attgatatga tatgggttag aatacggccg
      961 tttcaggcag gtgactctga cgcacttgtt tttgaataca ttcaagtctt taagtgacct
     1021 gctcaccatc atcaccacca ctactatcat catcacctcc actactatca ccaatgacaa
     1081 atcaagaaac tttaggctaa tagtatgaaa atacaggtga gacaggacgt ctgtctcacc
     1141 agcttctttt ttttgaattt gcttctaatt atgaaaattg aatctcagag aaggacacaa
     1201 tttgttaaag taattacatt cgtaactaga ttatctttaa ataataagaa ctagaagagg
     1261 ttttttaccc gttccccttt agtaaaaggt aatttccaat agcgatgcca tccatcccgg
     1321 tactatagaa agttgaaagt gcatccactg gcgatagaca tattggttga tcccttacat
     1381 ttagactagt gttagtaact attggaatgc cagtttcgtt ttcaaaatta cttattaaat
     1441 cgtagtataa tccattttgg ctttttgaaa ctgtctggac acttgccgta ttatctacat
     1501 gagtgggagc aggaatctcc tttactttat ctggtctaac attataggtt attgccataa
     1561 aaggtgcata tctacagttc atgaaatatt ccggagctct ctcttctaac acagacggtg
     1621 cgaatggtct aaagtcttct ctgaatttta tatacctgtt gagtctatct ttcatatctt
     1681 tgtcactagg atctgaaaga attgagcgag agccaagcgc tcgtgctcct aattccattc
     1741 tgccttgaaa ccagccaata atttttcctt gcgctactag atgagcaact tcctttgaga
     1801 tatcttttac acgctcaaaa cgaactttcg ctctcgttaa tacatattct atctgttcat
     1861 ttgtatattc tggtccccag aatgggtttt gatatttatc tattttaaag ccattttctt
     1921 ttgctatcat tatagctgct cctaaagaaa ggccattatc accgggtaca cttgtaagat
     1981 gaacattctc tacaaacttg gattccatca atttctgatt cattaaaaca tttagagcaa
     2041 cacctcctac aagacaaagg ttctgtgagc gagtcttttc atgtagatat tctaaaagat
     2101 tgagagctac cttttctaga tgttcttggg cactttttgc aatatcttta tagtatccat
     2161 ctatcggcat atcttccaga cgacgcggtt tattatttaa tttggataaa aatttctgtg
     2221 aatataatgg ctcttgcctt ggaggggtgc gattgttttc agtgacattt agataatcag
     2281 cgtttagaga gtaattacca ccgtctttat tattataaga gagcaaccaa gtaaagtcat
     2341 aattttttgc accattatta ttcccatatg ctgatagacc catcactttg tattcatcgc
     2401 tatccctttg aaaccctaga tattgagtga tcatggaata aaaaatcccc agagagtctg
     2461 gctgcttaaa ttcctttagt acagaaactt tatcctcaga gccataacag agtgttgttg
     2521 atttgccgtc gccggttaaa tcagctgtaa taatcatgga ctcatcatag ccggaagtat
     2581 aaaacacgct tgctgcatgt gccatatgat ggtctacgat tttgacctta ggacagaaac
     2641 caaaatgaaa tttaaaataa gtttccaaaa tacttgtgat gtcattatag gttatacctg
     2701 gaaacacaac ataagaaaca tcctgtattg agatattagc aaattggaga cagaatctga
     2761 ttgcatttct cggtaaaact ccatttgcaa attttatttt caaaaaccgt tcttcttctg
     2821 ctgctgcaat taatttacca tcatttaaca gacaagcgga ggcatcttga ttatttgaac
     2881 gaacgccgcc atttattcca agaatataca ttttctttta tcgttcactt gcatctgcat
     2941 ggtttatttt cagatttgat aggcaaagtc aattcacaaa aatggacata taggattata
     3001 tgagattata cattttaaca cgtcggattc cacagcctcg cgaggtcctt cttgaacaag
     3061 ctcaaacagc gtaaacatat gagcattaac agagttaatt aatacttaca tgatattact
     3121 caatattcga gtttatctta ccagtaagag gtattagaac atactataga ttatattttc
     3181 cattcttttg aaaatccgag atttatgttc ttatgttatc gctgggattt tgagacatac
     3241 gactacaact acccaatatc aaattcatgc atattatttt aaaaaataaa attccttgaa
     3301 taagcataac gtgtttatga taaaacgcct gataagcgat acaaatcata aattcgcacc
     3361 tgactggtga tatattgcca ctagattaaa tgagcacaat tacacataac cattacaatt
     3421 aagtatgtta ataattttag ctctacaatc cttttaaatg catgcttata aaacacagta
     3481 tggagcggcc ttcatcacat ttcaactgtg aaatctgtct ttgggcattt gaaaccaatt
     3541 ctgagctaga tattcataac tatttggagc acctgataat aaaccacgta tcacaaccta
     3601 gtagaattgt acaggatgtt cgagtgattg ctcgctgaac taaaaaaagg gattttcctt
     3661 tagtgttcca tcttacaaaa attcgctagt tcgtaatcat tgatactgct accgtgtggt
     3721 aaaaacatat ttgataagat gacatagtta gttttagctg ctattaacat aagctatatt
     3781 ctttggtgca aattaaattt ttatgagtac agataggctg atggtgcggc ccaccttact
     3841 aacagataga aaggctagat gattttaaga caagaagact cactgcaagg atatctccaa
     3901 aaaatgctaa cgtgagtaac acacggaaag gatcgatgct tannnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaagtggc gtgaatatca
     4201 tttgcaaata tgcgtaaaca caaaactttt gtattagtgc ctggaaaggt ttcccaatac
     4261 catgacaaga atgtctcggc gagattttct aaaatttatg ggtgctggag catttgtact
     4321 gacttttggg aggttcttcc aacatgctca atctttacca aataacagta catctaaaga
     4381 agcgcaacaa cttggatatg ttactaataa tttagttagc agtaataggg gaacacttcc
     4441 agtaatactt acctgtcc
//
LOCUS       DAOR01000096            3323 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_107922, whole genome shotgun sequence.
ACCESSION   DAOR01000096 DAOR01000000
VERSION     DAOR01000096.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_107922"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1033 a          652 c          615 g         1023 t
ORIGIN      
        1 agactgcagc aatcataatc gggatgtctt tgattgtcag cgttacaata aattgagtac
       61 ctacagttgt tacattagag caaatacacg gaagccgtga actctctgaa tcaattgacc
      121 agctatggaa cattattgca gatgtaggca atgacaccaa gtatcggaat catgtcaaaa
      181 ctatgacgat tatcaaaaag acaggcaaca taattgaagc aaaggccaca gttggcccac
      241 aaaattctaa aagtcatgag atcgttacat gttacatcca aaacaatcga tggtaacaaa
      301 tatcactgaa ggacctgtga taggtagtag agtaattaca ttaagtccgt tgtcggagaa
      361 caaaacaaag attgatgtat tatggagtgt ccatatgtcc ggtattcctt tttttgcaag
      421 aggttttgcg aaagataacg tcatgaaaca acagaggagg cactaactac aatcgcacaa
      481 gatgctgggt aataattgct cgcatcaacg gtaatacatc aagtgttttc tgttttcagc
      541 aaaattacga tagccaggta gttagttggt tagttagaac gcaactaatc tacatcgaaa
      601 gtgcaattac tacaccctca atcacatgcc tgtggacagg tatagtgaag aggtgagtga
      661 gataaatatg aaaaagattt aagaagaaaa gataaatggc gcaaagtcaa attcgttaac
      721 aattatagag ccgtattctc agcttttgtt atgccgccgt atttatctgc aatcttcgct
      781 gcctgtttca atgctctacc tctaaaatat gttgttagga agacatgatt tatgtatggt
      841 tttagacatc gacttatggg gtctattttt tcctgcaggc taggaccgat tgtcgaagct
      901 gttaaggtag atgttgatgt gggaatagaa gacagtcgca atctttgtta gtctgctgct
      961 gcgttttgcc gacgaccatc gttaggaaac attcggtata accaatacac gattgaagcg
     1021 cggggagtgg gattcgaacc cacgagtcct ttcggacatg ggattagcaa tcccacgccc
     1081 taccaggcta ggcgaccccc gcccctacgt atgcgttacc cacgcatcaa aatcctgcgg
     1141 cattatatta attataggcg agcctgccaa gggagaaagg gtagactacc cccgatattt
     1201 aaaaaggtta taaaaaggac atatctgctt atattatctt tgaaccttaa agagtcgaat
     1261 gagatcttcc ggagggccat aattaaggct tacttcgagc ctgaattaca aaaaatggat
     1321 tatcgtaaat cttctgctaa acatccattc attacagagg atggccttac agcaggtcaa
     1381 gacgttttac acttattctt tgatctcatc actgggaatg attatccaga tggtgatgaa
     1441 tggtttacga ttgagtatct actcccctat agcataaaat tacctgacaa gctaaaggga
     1501 cctgattatt ttaccaccat tatggcttct gaagaaaaac atttctggcg tcacagggaa
     1561 ttgatccgtt tcaaatacgg caaatctaaa aaactatcag agtcattgga atttatgaac
     1621 aagaaatatc gtgaactggc taaggcttta catgattcat cggcgattga cctcttgaaa
     1681 tgaatcgtag tgaaatgaat gacggccaca cgcatatctc caaccaaggt cgagaatcaa
     1741 caaactactc ccaactacta ggtagtgcca actatcacca caacccccca ccatcatcat
     1801 tttcgcttca attccttcgt ctttggaaga taatagtatt ctgaaccatc agagtacttt
     1861 gcgccgtacg gattccattt gaaatttatt tcatcgtaat gatatacaat ctcctccata
     1921 ttattagtag caggaaaaat ctgcatttgt atagggatga tgtcgactaa atattcaatt
     1981 ttttcaattg ccatttcaaa gtgcctatta tttttattca tgagcgaaaa aattaggcgg
     2041 ggctttattt catcttgtgc tattccacag aaggatatga atgccttgac gcctcttcta
     2101 cctcctcttc ttcgcttcat ttctttgatc aatcccttaa ttttgtacca cctttccata
     2161 gtgaatattt gaaaaaaaga ttctccaaat ggaatcggaa attcgatttc aaccatgcta
     2221 acatattgag tatcttctat tggaagctgc agctcgtcca caaggccaaa gacgcccctg
     2281 agtgcactat atattacttc gatctcaaac tgtgctatac cataatagtg aaatttggct
     2341 gatccctctg cactcaagat gtcaagttat tatagaaaag tgaaaattat aggctttgct
     2401 gcgtggcggc ttacctattg actgattaga agacctgatc tactcaactc gctctactat
     2461 tttgctcaat tcaaagttcg gtagtgctaa gaatcgcttt ctttcctttt cctctccacg
     2521 ctctgcaagt tgcgtctttg aataataata ataacgcttg actggataca gattcctagt
     2581 tttgctgctt tgtcttaatt tctggcaatt tgctttcgag atcagtttct gacatagagg
     2641 acaatacaac agtctatgaa ttttattttt tgattctaat atgtgtccgt tactatcttt
     2701 gaaccaagtc ataatctcac tactactatt actatataca ttgtcagtcc ttatttaaga
     2761 atttaagctt gcctcgctgc tcagcaaaca aatatgaata agctttatcg aaaattatac
     2821 atcatctctt attcattatt taagatataa ataaatccct atccgacatt aaaacaccac
     2881 ttcaagttaa tcatatggaa gctttgaagt tgatacttgc tttcattttt tataacgtct
     2941 agtatatttt ctgtaggttg atgcaatctt tttgcatttc ttgtaaccgt atctaaaatc
     3001 tcatcatgct ggtcttggtt tatcaatata ttgctggacg aaactgttat gcccgtagta
     3061 ggttacaaaa tgataattga tataattcag aaattctgtg tttctatggc tcgacatagg
     3121 aatatacatg ttccaagcca caatgctaaa tgttactatt aaaagaacta ttaggagtaa
     3181 tcgtgacaac tgttatctag gcaactagga cttatctaga atagtaagta acgggtaata
     3241 ttctaaagag atgaggaaaa atgccatcca cgaccacaag aaacggaatt tggattgcgt
     3301 atctcgaaga tgtacgttca ttg
//
LOCUS       DAOR01000097            4227 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_36752, whole genome shotgun sequence.
ACCESSION   DAOR01000097 DAOR01000000
VERSION     DAOR01000097.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_36752"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    426..704
                     /estimated_length=279
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1275 a          708 c          757 g         1208 t
ORIGIN      
        1 gggatatggg gtgaaaatac tcctgatggt aaaggagacg cgtttacatt taatttgccc
       61 cttgataagc gatgtagccg ctgactcttc gtcacgctaa actcgcaagg gaattcatat
      121 tatgtgaaga ttcaaacttg gctaatacac accggagctc atacttttgg tcgaatcccg
      181 tggatttccg atagaattca ttagcaatgt aagatatacc ccaccaaata acactattta
      241 tgataattgt agattgaaaa ccatagtggt tgtcataatt atcagtgcta tgttaccacc
      301 tatcagaagt ggtatttgat ggctccccat tgactttgta ataataatat ccattctatg
      361 tctaactctg atagtgatgt gtctattttc gttacgtagg aattgttatt gtaacgaaca
      421 gagcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncattgt caagcttggt
      721 aagcggtgac gatacgatca aacaataggc gatataataa cggaacctgt ggagatagca
      781 gagtgaaagg ccaagaaatg atctcgaaat ggggtcacag ggaatcccag tttaacggtt
      841 ttcctagtat acacttcaat atgttatacg ggctattact ccattatgtt ccattttgca
      901 gcttccattg tcgaatacat ctagtaatag taagatcaaa aaatcataga tatgtctatt
      961 ggataagtgg attttattgt aatttttagg ttatattata tagtcgtcta gtgtatgatg
     1021 tgtaatgaat gtgaggagat tttttgatgc aaaataattt ccctatgaga gaatggcatg
     1081 ttgaacatat ggaaaaaacg gttgtaaaat acgtgacagg attatcagag accgcctcta
     1141 tgtgggagaa aaaacagcac aagcgctatg ctagaatatc aatcgtttgc agacagatag
     1201 actatgatat aaaacatgga gttacaagtg agcaagttct tttgctttta caaaaaattc
     1261 gaactcattc ctcgttttta actcttctaa agaatgaagg atcactgaaa aggttagacg
     1321 aaatcatgga acattttgtg cccacccaga atgcaacgaa atggtggtaa tatctgacag
     1381 agtattttga ctaagtatag aaccagacga acacgaccgt acgaatctct tatatgctga
     1441 taatcgcgga agagccttac gatcatcttc ctcatttaaa aaaggagttt tttggttaat
     1501 tccattcagc ctcgtaatag tttcttaagt ggatatggta atcctttggc taatgcagta
     1561 tcttcggaaa tataaccaaa ggattcaaca gttttagcaa aacatttgaa tacacgatta
     1621 aatttgacgg aatacaaatt tccccaaatg atatactgaa acaggatatc gttacaatat
     1681 atgaaccgtc tagttatgtc atttccactt tgaaatacga ggccttatgc ttcaacatca
     1741 ccgtatcgga gattagaata cacagttaat ccttcaaaaa tcgacgcgac aaagacaaga
     1801 atggatattc cagtactctg gtgaaacgtg ctattgttat acgtcgattc aggtatataa
     1861 ctggtcgtca gttagcccga atgttaactt aataattgac tcaatgaata actctaaaga
     1921 tataaggact gacaatattt ctcggcaaag cgatattatt taataacata tgattttatc
     1981 aaagcttatt gctgtctctt attttagggt cacgaatgaa tcaatcatct ttcgtatagt
     2041 tggcaagtac ttggagtatt ttgccgcatc tgcatgatat gaaaggatat atgctttccc
     2101 gccgtttaca atccaaaatt ccatcgcttt tgcttttcct atgataggat cactgtaagt
     2161 gtaaacgacc tcgtatgcga tatgacttgt aatagttgta cttcgatttg attcaagaat
     2221 attaaaatct gggaaagagt cttttaaaaa agtaatttgt tcgtcggtaa atgtttttag
     2281 ggataattct cgggacgtca cattcgctgt ttgaattata agatactctc ggaatgtgtc
     2341 ggaaggtcct tccagtggtg aaagaaaatt aacaaccata ttccgagtac cttctgcaat
     2401 tcccttgctg aattctactt tttgccaact tgccggatat tgcacaataa atccatgatt
     2461 agaactctca tatgtcaata cattacctgc cagtgccatt gttgttagag ctgtttttgt
     2521 ttgggctgtt gctagttcgt attccggaat ggagagcatc gcagagaaca acaatagtag
     2581 caacaaagtg aatagagtca cagaatttga cgaagctctg acaaggtctc tcatattgta
     2641 ctctcggtca ctcgtacata ccagcattat aagatatatg agcctatact ttaccaaatt
     2701 attcgagaat cataatattc tgccagaaga tagtcaaagg attacagaag tgccagggtg
     2761 tcattagacg acactataca ttaacggtca tatctgtgag actaaaggaa aatataatga
     2821 agctgcacgc tgcccgattg ctggtgcatc taaagtatcc acattacttt agtaatgaat
     2881 cgagtgaaac atacatttgc agatactagt gcgattaccc ataccctggt gaattcaggc
     2941 atttgtaaca ttacgatatc ttgtgtggac ctactgaagg taagagggaa tagcgtttcg
     3001 caaaaacaaa ttatttttcc ctttgacata gcgccttcac tgccttaatg gaatttccca
     3061 actcattctt gattattctc tctttggttt tattttcaag gttaattaga tatgggtttt
     3121 ttgcagcaga taatgaccta cgtctcataa actatggcta cgagtatcca cctctgacgg
     3181 gcaaactcag actaacaaca tagatgtaat cttaaccaaa tgcagtgtct tacagtaata
     3241 ctgagcgctg aatggattat tgtctatgcc aaccggttta caaacgcaac ttcaaaataa
     3301 aaaatcaatt agatcactgg aatatgttcc tccacactaa gtcactccca cttaaataga
     3361 tccaccattt agacggacgg aaaaatcaaa acaactatga tagctaattt tcatagtgct
     3421 tggcaacact cattattctg ttcctcccat cacattgcac tgtttttact ttgggaatgt
     3481 ataaaggatt tagtctccga ttttcgagta aaaaaccact acctgcactg aacttgatta
     3541 cgtcgcgggc tgatactacg aaaagcttta cagttgataa ccttatagtt actaagttag
     3601 taacacatta ccaactataa aatactgaaa tctgtacctc ctaggttact attacaatta
     3661 tataagtaag gctccattgt atgccatatg ataaaaatat cccgcgacaa taatataacg
     3721 ctgatgatgt atatgcactg tccgaggaat ttggtgaatt gaagagaata cgatctctga
     3781 agccgccgat gatagtgatt tctggttcat gcataaaatt tgataaagaa agagaaaaga
     3841 taatggacta tattggagaa cttgaatggg gagttcatac gaagagtaaa ttcgaatgtt
     3901 cctctaatgg tagtgctctt ggctgggatt tctttcaact ctatttcgat ccggagtttg
     3961 tggaacgctt acttgaagtc catcccgata tgcgaaaaga agagggaaat atggttgaac
     4021 agcaatttgt acgctggatt tcaaaacagc ttaaaaaaag aaatctagaa tattatttga
     4081 aactaagcga tgtcccatac gatcacacac aaggtttcag actagaccct gaaaattatc
     4141 gtgatgacag cgaactagaa aagttacgtt agagatatgt aggtagtgcg cgtacgtagt
     4201 atgtatagta tcagaccagt gtagtac
//
LOCUS       DAOR01000098            8313 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_53805, whole genome shotgun sequence.
ACCESSION   DAOR01000098 DAOR01000000
VERSION     DAOR01000098.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_53805"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    6685..6841
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7938..8113
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2320 a         1602 c         1481 g         2577 t
ORIGIN      
        1 tgtaatccat cgataatata cgtacagtcc gtgctaccaa tgttgacatc agactatgcg
       61 gaggcctcgc ctgacgcact agggagctta ttgcctcgca cggcgaggct tcccaatagt
      121 ccagtatttg tatgtcgaaa aataacatct atagtccatt tcttgttact gtcgttactt
      181 cggggcattg ggcacaaatg atggttctgt ttttcgagat tggatgatag tcgtccatgt
      241 aattgcattg ttataaaaaa cccattagaa ggaaatagta ctagtattga tgcggtaata
      301 gatctggatt atttcctgta tcaatgatga tcccttgtct agcggtcctt gtatagggat
      361 taagggactt ctaccaacat accgggaggt ggggtagcaa ttcttgaatt acttgccgaa
      421 tgggtttcaa tgttattgtt attggtattg ttattgttat tgttattgtg gtggttgata
      481 ctgccagggc cgccaattcc tgcaattgaa ttttgagaat agcaattgtc cactatttga
      541 tattggttgg ggggtcctga tgggttttcg aaatacagct gtagaatttg ttaatttctt
      601 tgcggttatt ctgccttata ttatacttta taaataatca agtcctttcg gttgacactt
      661 agcattgata cttttcaggc tatgtcgagt aggctgaaca tgcagtcgta atccggataa
      721 cataatccag attaatagtt acaaaagcct atatagttat tttctgcctt ttactatgta
      781 tttgaaatca tctttaagac cattactaac caggcggagg aacactcaaa ggaaactcca
      841 gtttaagtct ttagaaataa ttgtgataat cgctctaaca ttcttgttat tgcttggcat
      901 accagggaaa tctgatatct atgctctacc gagcggcggt cattcacttg gaggtggtat
      961 tggcggtgct ggtggtattg gcggaggtgg tcccatatcg ggcaatcaac caaatagcat
     1021 cggctggggt catcgatcag gaccaaatcc aactacaaat tgtattgggt ctgctgatct
     1081 ctttccttca tccactaatt gtgcaagtcc aacagtacta catccagcca acgctcctaa
     1141 cacggctgta gtacaaccca gcaattgtaa tgtccctact tcggtacata ccaatactcc
     1201 caagaacatt gttgtacatc ctgccaccac caattgtgct aattctaacg tcgtacatcc
     1261 caccaacgct cctaacacga ctgtagtaca acccagcaat tgtaatgtcc ctacttcggt
     1321 acatcatacc gatgctcctt ctaccgttgt tgtacatcct agcaattgtc ctcccagttc
     1381 agggagctct aattccttaa cagttaacaa tgtacaaagc tccagtagta gtggaggttc
     1441 tactacaagt caaactacta tgactccagt cgcgaatgct ggttcaaacc aaaacgtaca
     1501 ttcatctgtt caagtcacgc tcgatggaac taagagttat gatccgaatg gccattcgct
     1561 tactttcact tggctacaat tagcaggggg tccagtcgtg gtattgtcaa gtgataataa
     1621 agctaaactg acatttacag caccattagt tagaaacacg actaatctga catttcattt
     1681 gatagtgaat aatggaaacg ctgatagtaa cccatcttat gagtctgtaa cagttacacc
     1741 gtagatcgat agttatacat actgatctaa ttaacggtac tgataataat ttactatgca
     1801 tttcttttta cctcgcatcc actttaacac aactattttt cgacctaaac tcatcagcca
     1861 ttacaaaccg aatttgcttc tttgtgaagg ttatatttct tcatgaaacc cttcttatga
     1921 taatgttatt acctgtttct gagattagat ttttgagatt cctgacttat tactaattaa
     1981 agctagccaa tgtacatgaa ttcttattgg cctattttct ctttatctgc ttctcaacgg
     2041 gccttctatt tattgagcaa ctcgaacaaa attgtagcct gatttctcga gtctattctt
     2101 tatctgttcg ttcgtaatta gtgaggggtt aaaggttact atgacactgt ctgtcatgca
     2161 gttaatatca acctttatta ctcctttctc atcctttagt tgcttttcta cgatagactt
     2221 gtaactggtg caatacattc cgactacctt gaacaaagct ctttcagttg tcatgttacc
     2281 ttcgtaaaag tacctctgat agttcattct tcctaagagc atacttacgg gctatccaac
     2341 tatgtgtttt gccgacatta tgcaattttt tgttcaacac ttttgctggg atacgagtgt
     2401 tacttaatct aattgcagca tatgtttatt ttgatagagg gaattgtatg cagcagaatt
     2461 gctaccgttc tttgttgtag tacttttagc tgagcccatt ctcagtcgtt acagaattcg
     2521 agtatataat tgcagattgt catgttggca taacgttcac ttactaacgt tttcatcttc
     2581 aaaccatacc tgtgcctcgt cactaaggtt agagcgatca tattttctaa gacaatctat
     2641 acaaagaaac attggggaat caatatatgg tctcacgtca gctatactaa agaaaccaac
     2701 agcctttcga caatgatcac atgttaagat agcgtcataa tcttttaaat cggtattttc
     2761 gctgtttact agagtaccag gaagaattgg attacacttt ggttcaagtg ctgcagtttt
     2821 aggattaaat acaaaatcat caaaactacc atttgcagta ttattttgtt gatgtatatt
     2881 gcttttattg ttatttgtca tgttactccc tctactccat tgttgtcctg taggcaatta
     2941 tttatctctt ccaagcatag tattcttaac ctaaatgttt gataaaactt accttagtta
     3001 cgaaagttct cactcaccat ccaccatgcc atcaaccaca caaaacaatg gaagatttag
     3061 gatgacctaa aaaagctgaa ctaattcaat ttctccagta tacataattt gttctagttc
     3121 tagcgagata agtgagaaaa agaaatatct agcgcaatta aatcgcgtat attgattcca
     3181 gtatagctga atactttagt agtaaaactg ctaaaatcac cgccatcaaa tgcgcagaag
     3241 ccaaaacttc aattgcgagg caattatcgt gatcatattg tatgtgacag gcgagcatca
     3301 tgtgcgagtc cttgtaccga ttgttttggt gcagtgcttg gcttggcatt gtagtagcgg
     3361 aaaagataaa gaaagccgac gcataatgca tgggttgttg gaaacgaacc tgctgctcga
     3421 ttttgaagga cttccctagc aagaggtagt tagtgacaag atttgatcca tctcctccaa
     3481 cagtcgtttg ctcactcatc ctatcttcaa taatggtaac gtcggtatta atatcatcat
     3541 ttcgttttat taacctaggc tcgtcttgat atcgttgtat tgaatactcc ttattattat
     3601 cccctgttca tagtacttta ttctcgcgcc tgaacctgat gcatggacat cctagaagga
     3661 aatttttttt acctagcatg tagatgcgtt ccctatggga ttatatggga tatatggaag
     3721 ccattctcca gaaagttgtc cgctaaacaa tgtggagatt agaagactta tcataaaaat
     3781 ggccggacaa cttgataagg ttctcaccaa aaacagcgta aagatgctac agcagtatca
     3841 ttcgggatta gaacacacgt ttctatggat cgtagacgca caagacgctc attcggtaca
     3901 gaactttatg gtcgaatcgg gatggataaa gtttaacgcc ttaaagatag ttccgttgac
     3961 atcttaccaa accgtcattg aaatatgcca aagtataggg acatgattga aggctgaggc
     4021 gcagagctta gaatcggatg tgtcccataa gcaactcgca cgtcacttat ctgaactgta
     4081 tcattgagaa tttttttgta tcatcgctta catctattac caacgaagta gcctcaacaa
     4141 ttaccaaagt caagatttca atatcttgta tccggggaaa ctaggagaat aggttgcaac
     4201 atagggacca gctccaattc atctcctaaa aaacataggt gctaggccac taggatatta
     4261 ttgggagcta ctgcaatgac tacaccattt ctgattgtcc taacattgct tcgtgcttca
     4321 cttcacacat caattttgga agaataaacg ataattcaag taatattgca tagctaacaa
     4381 tccgtgggag cggaagcttg tctggtgcga gagggctcgt ttaataagat aaaataggag
     4441 aacaattacg agcaaaccaa tacacatgat tagcagccaa ggtccagtat tattatgact
     4501 tttgaccgaa taaacaagat atactggctt atctaagaca ctaaacaaca atcctgcaag
     4561 tcccattatt ccgtttcggg caaatagtgc aaatagtgca attgaatgac actcctaaag
     4621 atcttactgc gagaaagctc acagcactaa ctactacgaa tgagatatgt tgtgctatgt
     4681 gtacctgttc gggtaattgc gcgaaatcga aaaccgaagg tatatgcaaa aatgccaata
     4741 tggcagcagc tatgatgatc cacacgtagc tatatctatt gactgaaaag attgcgcgga
     4801 ggaacttagt ccaagatagg atcagtgtcc ctagatcggt ttttcttttt gaatccgaaa
     4861 aaaccagaag ttttagaatt atttcagcga cctgtacaga tagcgcccca aaaaaagaac
     4921 aaagtatgtt acgccaaatc tggcgtcatt ctgtaaattc taagaaagcc ggttgaatag
     4981 acattacagt aagtatcgaa aagttggttt ctattaatga aatttggtgg cagatattct
     5041 cgcctttaga cgcgtgtttt tttttcgatg attagaatat gataattccg aagctactat
     5101 ggcagataaa ttattcaaag cacatccccc aaatagatac ctgcagaaat actacccagg
     5161 tatctttttt tctcatatga atctgtgagc gcaccaatag actccaatta tcgatccctt
     5221 catcattcac tactactaac ccccatttcc gccactagaa gctgcgatga gacttgccgt
     5281 catccatggg tgtatagtac aatggtaagg gattgttgct tgtcccttga ttatagctga
     5341 gccggaagaa ccaggttgta taataccggt atcaaaagaa ttgtcagtag cggtagctgt
     5401 gtgcggagcc gaatccttat tagtccaagt tattttggca ccactaagac gctagcaggg
     5461 ctgggctggt agaatatttt gacctgctta gtagcagctc cttgtggtat tgatatcttg
     5521 gtagtcaatt gtgaagctcc tccattgttg gtattattgg ttcggttatt agcaatatta
     5581 ctagtagtac tgctcgctac gacagaggtc tgttggtatg agctattact agcttgtctt
     5641 tgtgatgcaa ctgaagagac atatattgtg gcaatagctg ccaatgctat acctattcca
     5701 aatgttgctt tagagagaac agaattcaat aagtcattaa agttggcgtg gctcttatac
     5761 tatttgacaa atattaaagc tcaatttttt ggatgagtaa cccaatccga tcctaaaaag
     5821 aacatcaatc tgaatcgtta agaaacttgc cacaacgaga tgttcgtgat acaagatgat
     5881 tctgatcgtt ggacgaattt atgcttcatc atagcgtggt catcaattgg tcttgcttca
     5941 tgttcttaga ccggcaacct gaggatgtca gtttaggtgc attggattac cctgtagtca
     6001 gtatttttag tcgcgatact gataccgata cttttagcac aatccccctt agcaggagag
     6061 gaagcaccgt ccgcagagga agcagtatca gcaccaacaa tctgcctaaa caacgggacc
     6121 aacacacccg cgttaatgta aaagaacgcg ctagaaggaa agtaaaaaca catttacttt
     6181 ccattttttc tgagcatgta tggatctaat tttaactggc tccaggccgg agatttcagt
     6241 aaatgaaacc atctgaataa agaaacaact agtcgatgga tggaacggac taacctatat
     6301 ttgcaagaaa ataactgcaa gcccacagta cttgcttgag ttcacctggt accgcatagt
     6361 acataattat tctggtaact ctcccgtctc ccttatcgga gagtttttca cttttcacat
     6421 acaggccacg tccattgatg gtaaggtgtt tgtgtatctt ttacaagttg aaaagaccac
     6481 gtggtgaaaa cttatctgct cattaactat cttctaaacc ccagctaacc ttattgccag
     6541 gctgtacttt tacaaaggga gagtcacctt ccctccatgg atcatttctt acccattcga
     6601 actttttttc aaatatctta tacagttttt tgaattcttc gcctctttca accaaatccg
     6661 ctgtgccttg gattaccact gcttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 ngattaccac tgctttgttg tttagtgatg aattataaac atcaattata acggcgattc
     6901 tcttattttt ttcaagattt ttatatttta tagtattata atcagtggca aagtaaagga
     6961 aatcgttttc gtgtatgtaa ttaaccggta cgatatgtgg aatatcgttg tgtgaagtag
     7021 ctactctgca tacctcattt tctaaaagaa atgaaacttc tttttttgtg aattttaccg
     7081 gcttattact acctttcaaa ttatctatac ttgttgagag ccttaaaata aaaatctttg
     7141 tcagtgtaac tctcatgttc cctgagctgt cgttgtagac ttacactttt taatgtcatt
     7201 acataatttt acaagttgtt tatagtggtg aaaaatgatg aacatgaaaa ttgtctgtat
     7261 cctcatatgg cctggcagca taagatatcg ccaattgttt aagtgggcga tagaaaagat
     7321 ttggttaatt ttgaatcccg cccatccatc tgcaattgac tctgaactag cagatgcaag
     7381 acctcgttta gtaaaattga aatgactaat gatgtctagt taattcttgc tcacaatgtc
     7441 gtaacatcct aacagcattg taccaactca cgcgtctttg tcgcctgaga tggtggctgc
     7501 ttttatttgc tcaatggcag gctaaaagta aatgtggctc ccttttcagg actattgttt
     7561 tcaccccata tcttaccacc atgcgcctct acaatcctct tagatatgaa taaacccaag
     7621 cctgtacctg tatcagattt tgtaacaaat cttgtaaata accttgaaag tatcccagga
     7681 tctatgcccg ttccagtatc ctttacacta ataacaactt ctgtgttatt gtcgccctca
     7741 ccataaacat taccttctgt tttctctttt tctacattta ctgatatagt cccaccatct
     7801 gttttagtga atttaaaggc gttgtttatt aaattagaaa taacttgtat aattctttct
     7861 ttgtctgctt ctaaaaaaat atcctgaggt tgatataata tttgtacatt tgtaccgtta
     7921 tattgtgtgg ttaatacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnncatttgt accgttaaat tgtgtggtta atactatatc atctagaaca
     8161 tttaatatta catgatttaa attgaatcgt tcagtatgca attgtaatga ctggctttca
     8221 atctttgtga gatccaatag atcttctgta agccgattca atctttttgc atttctagaa
     8281 acagtatcca gtaactgtct cttatacaca tct
//
LOCUS       DAOR01000099            2653 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_60327, whole genome shotgun sequence.
ACCESSION   DAOR01000099 DAOR01000000
VERSION     DAOR01000099.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_60327"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          813 a          536 c          567 g          737 t
ORIGIN      
        1 gaacaggctt tggggggtaa cgataagagg ttttgtagcc ctaggatatc tctgatgtgg
       61 tggggatatc tacatacttc tctcggactg gggaaagtac gacttgcttg taataaaggt
      121 tgaggtattc caaatcaatc ttatatggga cagataattt ttttccgtct tttttagtaa
      181 gtatgcatta ctgcatcttc tgtttcaact ggttcaaaaa gcttctatgt cgatttcggt
      241 tcaatgaatt cggttcaatg ccctcccgta acatttgcca gcagttaaga agaagggtct
      301 gtgggacccg agaaatgcaa gatagctaga agtggaggtt aaaccgctgc gcttctgtta
      361 gcccaagtta ctatcgaaaa cgtactaact gcagatccga caactcctgc tattagcgct
      421 ccaattactg cttggttcag gaaatttggt atggccatag caaaccattg cgcgatgcct
      481 ggaagggata gatatccaag aaagcctatg ccaaatccta gaaaccaaaa agcaaagatt
      541 gcaagtcttc tagacaacct gttctcccct acatcttccg tatacggcaa ttttcgtaca
      601 tttcatttcg tcgctagatt ctttcatgtt ttagatgtat ctgtcagata ataagcctga
      661 gtgtcaattt ttataataga tcaaagacaa aaatgttttt tcatccagaa tttgacttat
      721 tatccttctc atgtcaaatc taaattagat gattccgtag ctgatatcgg gactagaaat
      781 atttctaatt atggtaaatt gttatgacca tcctaaataa atagaataat cttctcctac
      841 tcctgaacgg gtgtgtttat ccagaaatat ctacctcatc taacaaggct tattttttat
      901 gaaccaagca accgaaaaat gttcaataat ttaggtaata ttaataatat tgtcaataga
      961 aggggtttga atttgcagaa caaatatctc gacaggtcaa aatttaaatt tatgaacagg
     1021 agagcgggaa tgcgaatcag ctcagtgcct agcacagtga cgaagtgata tagatttgaa
     1081 ttcaacagaa tactatacgc agtgtcctga gtgcgaatct gggctcattc atgattacaa
     1141 cataggtgaa tacatttgcc aaaaatgtgg ttatgtagtc atggaccagg ttgacgatca
     1201 tggtcctgaa agcaattcct ccgactttga agaaaggtca aagaatacgc gtgctagcgg
     1261 atacacaagc ctttcattac acgattacgg tttaagaacc gagattggtt ttggttcgaa
     1321 ggattacagc ggcaaatcaa ttgactgcca aatggcagaa cagatgaata gcatgaggaa
     1381 atggcattca aggataagag tagcttcccc gaaacagaga agactctcaa acgtcttatc
     1441 caggattaac gaaacttgtt cagcgatgtg ttttccgaag atactggtgg aaacagcagc
     1501 gatgatctat cgaaattttg agagcaggag tggggcgaaa ggaaaatcaa tcaacgccat
     1561 ggcggcagca acaatttatc tagcctgtaa aagatgctct gtagtgaggt cgttagaaga
     1621 aatcgtggaa gcaactggca ttactcagaa ggataggtct agcctaaaac tagcttctag
     1681 atactatagg atgatggtta tggagatggg cgtctttaca gaacaaccca cgcagctctc
     1741 tacatcattg tctagtcatt tcccggcatt ggcagaagcc gctgtaggct ccgaatcagt
     1801 attaatttca gcagcagcaa caaccacggt aacgtcactt ccaatcccgg taacgcttac
     1861 aattgatcat tacatagcca aactcgcaaa catggcaaga attgaagcca aggtagagag
     1921 actagcgatt gagatagctc acaagtccaa caatcacctt cttgcagatg gcaaatcccc
     1981 aaacgggctg gccgcagcat atatttatct agctgcaatt ctactgggag tcaatctttt
     2041 acagatagat ttatcaagct tggccggagt gactgaagtt accatccgta acagatgtaa
     2101 agatattttg acaagtttta agctaacgat aaaagtcaaa ccgttaatag gaaagacagg
     2161 ccgctcatga ggtccagggc cagagattca ttcttcttgt tttctatata ttattctggt
     2221 ctactgttct aatctgccgt ctgcacgtag agactaattc gcctacgtgg gctggctctt
     2281 cgagattctt ctcgaacgga ttttgagtgc gagaccctat ctttcagtaa atcgtccctc
     2341 cgggcagttt ggaacgggcg cggaattata gcgctatttc cgaggcgagg aattatatca
     2401 cgacatttgg ctctaactat acgcccactg catcgcagaa tcgtcccgca cgtctatatc
     2461 gtctttagaa tgcaaactat gccactggcc atcaaggttt tgataaatca cgcccagcag
     2521 cacttgagca gcgtcatcaa gtggaaataa ttagggatat actcacaaca cccatgatat
     2581 gtaataactg ggagaagaac tggaaagagt tattgagtta taccacttac tataggttga
     2641 caccaccatg aat
//
LOCUS       DAOR01000100            9814 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_31295, whole genome shotgun sequence.
ACCESSION   DAOR01000100 DAOR01000000
VERSION     DAOR01000100.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_31295"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2943 a         1871 c         1906 g         3094 t
ORIGIN      
        1 tgtgtgtaag tacaacacct agtcctaggt tatctatgat aaaaatttgg cctttcgtca
       61 tgaaatatgg agggtagctc aggttaatga cttcgtttct ttaggtgcag ctttaggttc
      121 ttgcagaatt ttgttattat cattagtttt ttatcgagat tctgtcttct tgaggttact
      181 actacttggc ttttgagtat tatcaaaact actttctata tcagaccttt ttatcattgc
      241 tacatctggt ttggatatca gatcagaggt ccaatctccc ataactttta atttcttctt
      301 tatttgttgg aaggtcacct aaatagtatg tccgccaaag ccaccatgct gcaaatccat
      361 gtaatttgaa tcggagtatc gttgcaacac ctgttctttt tccaatttcc gccatcattc
      421 cttttgtttt ataatcaaat ttagtcttcc tattttctct ttcaccttta atagaagaaa
      481 ttatattttt tgcaaactta cctagtggtt cagctatttg ttcgacctgt aattcctgta
      541 attcaggtac ttttagctta gagtctggac aacaactaga tcggatagtt tctgcagaca
      601 gattcttttt taacataata tttggataaa gcgaaacaaa gtcaaattca gctacctgtt
      661 catgtactcc tatttcaggt tcaaagataa atccagctct atctgcgaca agaagatcct
      721 caaaggtctt aaggtgttcg gctaacgctg gcttccaagg tattaaaatc tctttttgtg
      781 tggtataata aaactgcaaa ctactcaaac actttccatc gaagctctag ctgctgtacg
      841 aagtggcatc ctgcaaattc tagctatttc atataaacct tgtaaacctg cctcattaca
      901 aacaaatgaa ttagatttgt ctaggtgtat tctaccaagt aattttatag tagctggttt
      961 gaagtgtatc cgtccatatg aaaaataaga gatacctttc gtggtaggtt tcttcaaagg
     1021 tatggctggg tctctgccaa gaatcaggtt agtaccattc tgctcagctc tatgaatcaa
     1081 ataaggaaaa gtgaatgaat cgccatcttc tgtgaatata aaatctggat caatcctctt
     1141 tgttatctcc gttacaaatt catcgatcat gttggcttct gattcactct gaatttgaat
     1201 aatagtggct tgggagtctt gttcttcttg tttaattaaa atcgattcga ttttatcagt
     1261 gtgtttaggt atcattcttt ctttagattt aatttcagat gaacggtttt taaaatctgg
     1321 cactttgtag tccgttgacc agacattatc tttgatgttc caacttgaac aataacaaca
     1381 gttattattg ttgagtttat aaaatgcaag cggaaaaata tcgtgttcgt agaagtaaga
     1441 ttgcactggc ggtaaattaa cattgtatag tctaaacttt ccaatcccat caccatcaag
     1501 tgttctttca atatctcttg ctaacattaa tgcttttgta gagtccgaca atgttaattt
     1561 cagaacttca gacttagtat catctgtaat tctttcatat caagatacga attcgtggtc
     1621 tttaattaac caaccaatat tattattatt ttcactttgc aactattact gataagattg
     1681 acttcagatc tgacttgtta tccgctgcaa cataaattga attactccaa ctgtcttcta
     1741 atctgttagt attaccattc ttttgcttta cccaaaaaac cattttatcg cgtagtgaat
     1801 atgcatcaaa caaccaccac tcaaccaatt ggttaaatac atccttccgg tcgtccacga
     1861 caatttgata atagatacat ttcagggggg tacggtaaag tagggtatct aataaaccat
     1921 actaaagata tactttatac tgtccaattg aagcattgct gcgagaccgc atacgtaagg
     1981 ccgagcgtct tgccatataa gtaacacaag aacttttaga tgccaagata aacactgaat
     2041 tagtgtttag gtgaatttcc gcattgtgca cattaaaatg atgagacaaa ctaagacaaa
     2101 agatcgagca aattcgaacc ttccaagcat taaagttagc cctaacagag aggcattgaa
     2161 ctattactac ctttggtcca attcgtgtaa taatagtaat aaccttgata agaaatgtac
     2221 cacatatatc aaaatcaaat aaataaacaa caattaatcg atctttattg tttattacga
     2281 aatctaacgc atacatattg atatacaact acaaagcatt cacaaatggt gtggcgcata
     2341 agatcgagga ttacattcaa agtgaggtca aaaaatatgg cacgttatgc tttccactta
     2401 tagattctga aaattctgct ggggcagctt caattgcaaa aaaggttgaa ggaatgggag
     2461 cttcagctgt tttagtaggc ggatcttcgg caatcgatca acttgaactg acggaaatag
     2521 tttcgagtat caaactacaa attaacattc ctgtgatatt gtttcctggc aatgttaccg
     2581 gagtatcgcc cagagctgat gcaattctat ttagcactct acttaactca gaagatccct
     2641 atttcattac agaagcccag gcgttgggag ctctatctgt caaaaagtat aacatcgaac
     2701 cgttgccaac tggttacctt attatcgggg agggtaccac tgcatggttc ataggccggg
     2761 cacgcggaat tcctttcagc aaatatagaa ttgctgtcat gtattcgctg gcagctcagt
     2821 atctaggtat gagatttttg tacctcgagg ccggttcagg cgctatgcag agtatctcgc
     2881 cccaaatggt atctgctgtg agaaaacact acgaaggaat acttatagtt ggaggtggaa
     2941 ttcgtgctcc tgatacagca aaggaaatag ctaaagctgg tgcagatgtt attgtaatca
     3001 gtacgatgat tgagcaagat ggaagctggg aagataaact ctctacaatt atcaagcaga
     3061 taagaagatg accacaaaac atgatataac aatgatcgat gaggctgagc tccgtctaaa
     3121 agatgtgcgt ataccagctt attatttaaa ttaccaacac aatattttga aaaatgtaca
     3181 aaagaatttt gaacttgctg cgaaggcacg gaaactggga ttagatgttt cagacggagt
     3241 agagccaaaa attgcgtacg atctagctga tagagttgca aaaatgcata atattgatat
     3301 tgcagatcga ttgcgtgttt tactctcgtg gtcaaccaaa gagaaggcag cacttacaat
     3361 tgccgaagag atagctttgg cagaatatgg caatggtgac ttacgtacga gaatggacaa
     3421 tgctgtacgt gttagtctag ctatagttac cgaaggtatg acaattgctc ctttacaagg
     3481 aattgctgat gtgcaactaa agagtaacaa tgacggaagc caatacctgt ccatatcgtt
     3541 tgccgggcct atcaggtctg ctggaggaac tgaggccgca tttacaatgc taatagcaga
     3601 tcacgtaaga aaaattactg gcttagaaaa atatgctgca aattcatacg atgacgagac
     3661 aggaagattt gtcgaagaac tcagaattta tgagcgtgaa gttggaaatt tccagtttaa
     3721 ggttttagat gaagatgtag ttaaatgtat aaccaactta ccggtggagc tcgatgggat
     3781 agatactgat ccggtagagg ttttgggtca tagagggatg aagagaatcc ataccgacag
     3841 ggtacgaggc ggtgcgctga gggtaatgaa tgacggtctg atcggtcgca gcagaaagtt
     3901 actcaaaatg gttgagacat taaaactaga tggctggaat tggctcaagg aactgcaagg
     3961 agctattcaa acaggcaatg atgatgcggc taatcacaga atgtctgaag tgattaccgg
     4021 taggccagtg ctttcaatga gtaagaaaat cggtggcttc cggttaaggt acggtcgatg
     4081 ctacaatact ggtttggcaa ctatcggaat tcatcctgtt gtgcccatat tattaaatca
     4141 tgccattgtt gtgggtacac aaatcaaaat agatgtaccc ggaaaggcat ccagtatagc
     4201 acttgtagac accattgagc ctccagttgt taggcttgat aatggtaatg tcatacaggt
     4261 atcaacagtg gaacaggctg cacggatccg acctaacatc gacaaaatac tctacatggg
     4321 cgatattcta ataagttatg gcgattttct tgaaaataat gctcagctgc tcccagcgag
     4381 ttatgtggaa gaagtttggt cattacagtt acaggcaaaa ttgctggcat tgcaaacctc
     4441 ggactccatc ctaactggag aaaaattaag caaaagacta atccaattat caaaagagcc
     4501 tttctcaaca gtacctacag ttgaagaggc atttgaaatc agcagaaggt ttaacattcc
     4561 acttcatccg agatattcgt tctattggga ttgtgtcagc atcgatgaag ttttattact
     4621 gagagaaaaa tattttcagt atcaacaaaa tgctaagcta actgataaca gtagttcttc
     4681 ttattcttct ccttctcctc ccgctttaca attcctcaat gataatccaa agttaaaaga
     4741 tatcttggaa aaacttggta taatccattc agtaaaaaat gagggtaaaa ttattgagat
     4801 cgataaccta gatcatgcgt atacgctaac caagttactg atccctactt ctgctgagag
     4861 aaacgctgcg tttccgtcat catcaccggt tggttatgat gccgattgct ttcttgaagc
     4921 taaggataca ttagaattta tttcaaagac atccggagtt gaaatcagag caaaatttgc
     4981 ctcttcaatc gcagttagag taggacgccc cgaaaaggct gctgaacgta aaatgaaacc
     5041 tcctgttcac gtattgtttc ctattggtat caaaggaggc gcaacacgcg atattttaag
     5101 agcagcaaag gatgaatctt tttatgctga aattgcaaat agattttgta acaattgcaa
     5161 actgccttca attggaacac gctgtcgtaa ctgttatgct cttactccca tacgaaatct
     5221 atgtgtagtt tgtagggagg aaattacaga tggcattaat aacaatcggt gctataggtg
     5281 tggtaaggat ggcaagactt actctcctgt caactattca ctaaaaacag ctgttgaaga
     5341 ggcgcaaaga aaacttggca ttatagcagg agaaccacta aaaggtgtta agggtcttat
     5401 gagtaagcac aaagctgccg agctattaga aaaaggaata ctgaggcaaa aatttgatct
     5461 ctatagtttc aaggacggga ctattagatt tgatgcaacg aacgaacctc tgacccactt
     5521 taaacctgag tggactataa gtagtataca aaaattaaag gaattgggtt atacaagaga
     5581 ttatcttggt agagagatag tatcggctga tcaattggtc gaactgctga tgcaagacgt
     5641 ggtactacct ttggattgcg ctaaacattt gctcaatgta gccaacttta ttgatgaaga
     5701 acttgtaaaa ttatacgggc ttgagccatt ctataactca ttctctgtag aagacctgtt
     5761 agggcaccta atagttggat tggcaccaca tacatctgtt ggtattatag gaagaattat
     5821 aggttttacc aattcccagg tctgtttggc ccatcctgta tggcactctg caaaacgcag
     5881 ggattgtgat ggtgatgcag actctataat gctattagct gatgcgttcc taaacttttc
     5941 atatgatttt ttgccggata aaattggtgg actcatggat gccccactac ttattcaacc
     6001 gattgtatta ccaaatgagg tacaaagaca agctcataat attgatgttg cctcagttta
     6061 tccgcttgaa ttttacgaag caactttgaa acaagctaag gctggagacg ttgtaaacaa
     6121 gatagaaatt attaaaaaca ggctcggaaa tgaagaccaa ttttttggtt atggatttac
     6181 ccattcgaca agtctgctaa cgaccagcat acaacgcagt gcatattcta gacttaattc
     6241 catggatgag aaattggaaa tgcagctcgc tactgcaaaa ctaatcaatg ctgtcgatcc
     6301 tgatgaggta gcatcaatgg tgctgacgac acatattttg ccagacatta tgggcaatat
     6361 gcgatcctac tcatcacaat cctttcggtg tagcaattgt ggagaaaaat atcgcagaat
     6421 gccacttatg ggcaggtgta tggaatgtga acatgagctt ttgcaaacag tcactagagg
     6481 ttcggttgaa aaatatgccg atattgcaat aaatatgtgt gctcagttca gagttaatga
     6541 ttacttacgt ggcagagttg aatctctaat gacggaatta aaattgatct tcagggaacg
     6601 aaagaaagag cagtttactc tgactgaatt tatggattaa ctttggtccg agataatatt
     6661 ttaaatagtg acgtataatt ttgagagttg catctgacta attctgtaac tttgaatttt
     6721 gtatttttta tttagtgcat acaatgggtt catgcatttc aatttaagat gacttacagt
     6781 atgccgccat ttttgtataa ttagaattat tggtaaagaa ataaaagaat tggatttata
     6841 agataagaga agttatggaa ttgcttctgc cagtcctcct gcccctcctg ctgctcctat
     6901 tggttgcaac cccttctcgc tcaatacttg attcagaatg gttagatcgt atatccctga
     6961 caggtctggt cttggttttc cctttgccaa gaatccaata tcgtaagcat tattggctcc
     7021 ctggaagagt gataacttta gtggatcata tgtgaattgt attcttgacc aagcttgctt
     7081 caactcattt tcatttatgg ttttacctgt taatttttga aattcagtat taaatgtctt
     7141 tattgccgcg tctgtatgat tatttatcca tacggtctcg ttaatatgtg ttgtaagtag
     7201 tttcttgact acatcaggat tttgttgcaa gtagtttgtt ctggccacta tattggctgt
     7261 tacaaactta ccatctggtg gccacaaggt tgtttcatct accaatattc ttcctccagc
     7321 ttcattaact aatctcgcac cccaaggttc cggtacccat gcaccatcta tctgtttctt
     7381 taaaaataat gtaagaataa ttggattatc aacaggtgtc acagttacat ttcctccatt
     7441 gtccttcgtt ttgtatccat tatcttgcaa atattttcga agagaaatat cctgcgtgtt
     7501 tccgagttga ggtgaagcaa acactttatt tgcaaaatcc ttaggagatt taattccgga
     7561 atcatttctg actacaaaaa cagcaccacc acttgccgca cctgaaatta ttctgagatc
     7621 cgttccttga gatgctacat agccatttac tgctgggcca gggccaacgt acgtgacgtc
     7681 tattctattt gctagaagag cttgaatagc tgatgaccct gcgttgaata tgtatggctg
     7741 tacttgtata ctgcttccaa gttccttttg gaattctcca ctcccaaacc cgattactgc
     7801 ttgagcatgg tttatgttag gaaaatatcc tattctcaaa accttcgttg cgctttgagc
     7861 atgtacaaat cctgtaaccg gtgtgtgcac cataactgca gcaagtatta ttacaagggc
     7921 acctatcgtt agttttttta ctattttttt atccatagtt tctggagatt attttacatg
     7981 aatattattc atcctatttt atatgcaagt ttcaagatgc aaacttaaga ttatgcgtgt
     8041 tcagtaggca tagctctttt aaaatacgcg aaaattgcat cttgtaggtt gatgtggcgc
     8101 attcaaagtt agttattatt tgccttaagc acggaaaata acaatcatta gcttcaacca
     8161 gaaggaacct gtctaaaaga ctatgaagta gcggcaacgt gtgccaagtg ggatcgacgt
     8221 gcatgggagc gtacaactaa tgtagaggaa tgttatcttc taaagagaga gtttctatct
     8281 attttatttt agcctcatat actatgttga acggtgtctg cgtagccctc ctaaagtgtt
     8341 tgaaacctcc atcctttact accttcaaaa tagcgtcctc ccctgcctga gcacccatcg
     8401 ctggcccatg atatgctaac gaagcaggca cacataccat agttgatgca gcataaaata
     8461 ccctacttgc tggatgtttt agattgtcct ctagtttatc attggcaaaa ggttcaacta
     8521 tcattaatgt cccattcggt ttaagactgt ttagagcatg agcagcggca ccaataggat
     8581 cgcccatatc atgcaagcaa tcaaagaatg cgaccaaatc atagtcatta ccagggaaat
     8641 cagtagactt ggcaacttca aatgctactc tatctgctac tccttcttca ttagcttttt
     8701 tgcgcgccca ttcgatagaa ggtacatgat aatcaaagcc aacgaattta caattaggat
     8761 aggccttggc catcaatatg gtagaagcac catgaccaca accaacatcc gctacttttg
     8821 cacctccttg tttaaatttc tcttccactt ttccgttttc aagtgcagga atccatgaac
     8881 ttaccaagtt ggagacataa ctgggtctaa agaaacgttc tgtgccttcg aacagccctg
     8941 aatcatgttc gccccaatca acaccaattc ctgttttaaa cgcatttgtt atttttggtt
     9001 catccttatg taacgacatg gtgatttgaa aagcacctag agcatacact ggactttttt
     9061 cgtctgctaa agctagtgcg tgttctggag gaagggtgta ttctgatgta ttggaattat
     9121 acgtaatata tccacttgct gcctgatttg ccaaccattc tcttacatat ctttcagaag
     9181 tattagtttt cttggcaagc tgagtagaag taaatggttg tcctgctgtt cccatagctt
     9241 tgtataaacc aagtttttct cccatgtaca ccagaattgt gctccaagac gccccgatat
     9301 ccgatacaat ttttcccata aactcattta gctttgattg atcaattatg gttgatgtca
     9361 ttgtattatt ctcgctattt ctccgcgtat tataggcttt tctcatctac tgatcctgta
     9421 aaattgcccg cgatgaggat tgttttatta gttactccct tattggactc ccttaaaggg
     9481 agtaacaatc ttttagatat tactctaaga cttgatcatt cccatgaact ttctaattcg
     9541 ccgtttgagt tcgtctagat acgtaatgtc aaagtcgatt gaataataaa atagatgttt
     9601 caaaggagtg ccttgtacgt cacgactact atgcgactat ctttgcatat ccttatgatg
     9661 cgggttcgga caatattact cccttactgg actctcaaag tgaggaattc ttatagtttc
     9721 atactgcagt gttgcataac ttgtgaacct actatatgat attgctttta caatctattt
     9781 tcttagaata aacgtattgt acaacgatgc tttt
//
LOCUS       DAOR01000101            3447 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20774, whole genome shotgun sequence.
ACCESSION   DAOR01000101 DAOR01000000
VERSION     DAOR01000101.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20774"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1230 a          549 c          694 g          974 t
ORIGIN      
        1 aatgttcatt tggacatacc tcatgctttt acatcgggcg gcatgcaagc aataactgac
       61 tatatcagaa catttaggga aaagatcgtc catattcatt ggcatgataa ccatggtaca
      121 tatgatgaac atttgccaat tggagaggga ttaattgatc ataagaaagt agttaaggaa
      181 ttgaaaagca ttaattacaa aagaacaatt actttggaag tatttacagg caaacaagat
      241 gcaaaagatt ctgcaaacaa gttaaaggag ttatggatga ctgactaatg tgacacataa
      301 cttcttatcc cagcggtgtc ttcatccata aggccaaaca tctattattt ggcagttatt
      361 gatgcatgaa ttttacaatt gatttttcta tcctacgact tctattagta atgcattgag
      421 ccggcgacct ctaaaattga tggcgtaaag aatactaacg gggagcttta gaggtttaca
      481 atatccaagc cctgatcgtg ttttatcttt gaaccacaga cttcttgaag ggacacagca
      541 cgggattatt aatgtacatt caaagaattg ttcatttagt cttcgttcaa ccagtagtat
      601 gaaattctgt taggttgtcc cgcactctat agtaccaatt aggtagacta atagattaga
      661 tccgcttttt taacgaaaac attattatct cgatctagag aatttgacca gctatgtcta
      721 aaacaccgat agagataagc gacaaagctc ctgactttca gctacctgat attaacatga
      781 agatgcacaa attaagtgat tatctaggaa agaaaacggt ccttgctttt tttcctgctg
      841 ctgagtctcc aacctgcaca gcggagatgt gcagttttag ggattcaatg acagacctac
      901 aacggagtgg tgctcaagtc gtaggtatct caattgatgg tccatttgct aacaagatat
      961 ttacgataaa tcgtcacctt aacttcgacg tattgagtga ttacaaacgc gagactataa
     1021 acaagtatgg ggttgtaatg aagaatttag gcccattaga agggtataat gctgcaaaac
     1081 ggtcgatttt catacttgat gagaatggca atataactta caagtggatc tcagatgacc
     1141 caactatcga acctaattat ggggagatta atgcggctct caagaaaact cgctagaata
     1201 ctcgcatagc gctaacgtaa actgactttt catcaaaggt aagaatcaat atttgattac
     1261 gttctagcca caatctggag atatttgcga gttctaaata ttcctttact ttgaggacag
     1321 aaaaactgag agaagccgga atattcatta ctggcaaaca gtaacattat atacttttta
     1381 ttgctgatat tccaaaggct cggctagttg tacgagtcct ttttatgtca aaaaaaccca
     1441 ctaatattat atttaatgtc attttttgta acttctagat agagtactta tatatagttg
     1501 cgttttgaat ttttgtacga gtgcctattc ttacggctgg tcgagcaaga aggacagaag
     1561 tactgcatgg aactgaggat gttttagacg cagaggtacg attcttctct aatacaaaaa
     1621 gaagaatcga tacttgtatg aactatacgc gaccaccgtt agccgtcgga attggacaaa
     1681 ttaagaaagc ttttctcgat gctaaaagta ggggcgtgag attaagatat cttactgaga
     1741 tcacaaatga gaacatttct tattgcaagg agctaataaa aattgttgat gagcttcgcc
     1801 acttggatgc aattaaaggc aattttatga ttagcgaagg agaatacctt gctcctgtaa
     1861 atttggatga agaaggaaag atcgcatcgc agcttatcta tagcaatgta gacgaaattg
     1921 tagaacagca gaattacata tttgaaactc tatggagtaa agcaatacca tctgagcaga
     1981 gaattacaga gattgaggag aacaagactg ttccaagaac tgaggtattg tacggtgctg
     2041 aaaatgctgt tgaaagagga gttcaattta tgaaaaatgc aaagaaaaaa atggatatat
     2101 tctttgatag taaagcccct tctattgtta ttgaaattga tgcatacagg aacggatata
     2161 tggatatcag aaagggagga ggtaaaataa gagcctttac tgaaattacc aaggataaca
     2221 tccactattg taaggagcta ataaaaattg tagatgagct tcgacactta gatggaatca
     2281 aaggcggcat agcagtaaat gaaacagagt atatggctac tacggttctt caagaagcaa
     2341 aaccactgac gcaagttatc tatagtaata tgaaggaagt tgtagatcaa gggcaatata
     2401 tctttgatat tttttggtat agggcaattc ctgccgaaca gaaaattagg gagattgagg
     2461 atggaattga accaagtaga atagaggtta ttccagacac gaaggtatct ataagtcgct
     2521 cagtagatct aataaaatcg gcagtaaagg aggtactggt aatctttgcc acgtccgaga
     2581 ccttttctct tgctatgaat atgggcatat tacaattata taaagaagta actcagaatg
     2641 gggcaaaaat aagattatta attcctgaca gccaacaaag tcaatggata gtaaatgaat
     2701 taaaatcagt agtgccacaa gtagatgtta ggatagcaga taagagttta cagactcgaa
     2761 ttacgattct tgtagtagat aaaacagaat taatgatatg ggaattaaaa gatgatagct
     2821 tagaagatcc atatgaggca ggaggagttg caacatattc taataacaaa tctattgctt
     2881 catcatatgc ctcgattttt gagaatctct ggaaacaaac tgaattatat caaaaactaa
     2941 aggaagcaga caaaattaag gatgaattta tcaatgtagc agctcatgaa ttacgtactc
     3001 ctatacaacc aatacttgga ttagctgata ttcttcgcac caaactagga gatcgaaaac
     3061 aagaaaagga gtatcttgat gttataatta gaaatgcaaa gagactccaa agactttcag
     3121 aggatattct agatattacg aagatcgaaa gcaaatcact aggtctaaaa aaagaattat
     3181 tcaatctaag tgaggtacta ctaaatgcca ttgcagattc caacaatcag attgttaaag
     3241 aaaataaaga tcatcatcta aggttacaat tagtagcccc agaagaagat atatttatgg
     3301 aagcagataa gggcaggatt aatcaggtaa tctcgaatct tctaagtaac gctattaaat
     3361 ttacagggga aggaagtata attctcactt tagagaaaaa agataacaac caagagattc
     3421 ttgtcagcat acaagataca ggtactg
//
LOCUS       DAOR01000102           10974 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_31104, whole genome shotgun sequence.
ACCESSION   DAOR01000102 DAOR01000000
VERSION     DAOR01000102.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_31104"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2512..2557
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9284..9470
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3279 a         2092 c         2049 g         3321 t
ORIGIN      
        1 acttgtacgc atgatttgta cactagattt gtcctgcatg ctaatcggtc agactccatc
       61 tggaatagct gcctccagaa tcgacctagc tatctcgata ctacttcatt atttcatctt
      121 gtctatctga caagaaatta caatcaggta cttatgaaaa ccttaacata ctaaaagtta
      181 tgtcgatttc tgacaatatg tcgttgttta tcaatcgtaa ctatttctca atacaagact
      241 gtatgttgga gtattttttc cagtgtcccg gtctgttggt ctgctcctgg aacgttttcc
      301 cggtgtatct gcctcagtta ttagccggga tgttttgtga ccttttagac gagagacata
      361 gtacataata tttttttagt ttgtattaaa ctcttgcaat tatttgtgaa tgattatatc
      421 atattcgaat ctgatcattc taatcagatt tatatatcat aaatcctttg tattggctat
      481 ggaggtggga ttaaaaagtt gtcggtggaa caaggcatat accctgcgtt cggagtaggt
      541 gttccgttag cggtatgggg attcctgtcc tatttacgag agaattctaa aagtaagaat
      601 tataatgaaa gtagagctaa ctccgaaaaa aaggaaggaa aaaaagtaaa gaggtacact
      661 tctgatggga agccagtcta tgagtaacta ttccttgttg tacgcacgat agtccaacac
      721 gttggtcttc caaatctctt ggaataccaa cgtaggataa gacgacctac agctcaaccc
      781 cgcataacat gaataacaat ttagccaata acattgggat aaccgcattg accaataacg
      841 ataccttttt gaattaacgt tacgtgagga atatgctctc cccttacatc accgtaccat
      901 gagagatctc tccccaaaag tctaaggaga taaaattggt aaaacacgac tgtgatagac
      961 ttctctccct tttcgcgttt gcattttaac cgtccaagag attaacgtca ctcctaaata
     1021 gtacggagca acttaactac tcacgcttca attcacttaa atacagatat ggataatgtt
     1081 atccggttgt ttctgaaaaa tagaagcaag gagtcaaatg atgacaacaa taatgcatct
     1141 tggtgtcaac tctttattga tgactacaat agtactagag taaaaattat acacataaag
     1201 agaggtaaat ttcaaattat tgacgacgtg gaagggggaa aatatgttgg taaaatagtt
     1261 gacgcatccg acatacatca ctgcgaagtt taattctata actttgaata ttggggtatg
     1321 ctgcaatttc gaaagctgca tatcaaaaat taagccactg gaaagatttg atagcccaaa
     1381 ccctgcaaga actgtcgtgc tgcagaatcg atggcgtgat aagggctgct gagtgatata
     1441 ctttcgctat cgatcttgat gatattctga ttcgaccatt ctctaaagct ggcgttattc
     1501 aatgaacgta catcttcaag atagtttggc aatatttcta tgaatttaaa accattttct
     1561 acatcaaagg aaagaactgg atcatggatt tccccattga tgaccttctc agcaaattct
     1621 agtggagacg atttacttga aggttcacaa tagtagaata atcttgcgcc acttacgatt
     1681 tggcgtaagt ttaatgtctt gactatcttt ttccttgcat cataaagcat cgttccaatt
     1741 ccttcatgtc gaacttttgg atgagtagat atctgctcca tacctgctct ctccttttgg
     1801 attatgattc gtgttcgtga tcatgccatc ggcagtgatg tctctccatt tatgttctgt
     1861 atactcgggt tctagaaaga taaattaggt tactcgcaca aggaagtacc tctgcattta
     1921 attcagcaca aaattgtctt tcggaacgag tctaagatga ctttgaagct cttgtggatg
     1981 tcaaacattt ccataacgtg ccaaataacg aaatgaaccc tcttgaagct tgactacctt
     2041 ctggatgtct tcgggcagag cattgctaat catgatcaag gtattattct tcttcatata
     2101 ctctactttt gtagtccgtc cttttttcat ctatgaggat tagattatcg tttattgcca
     2161 tgacctttgt aagtaatttt accagttgat ttagatatct aaacggcaat tatcatcacc
     2221 tactattcac atatatcatg aatggcctaa tcggagagac aaggttaaga aagtgaagca
     2281 gaataaaatc gataacaaca tctaatcgat ggagctagtt ggtctctggt atttgaaaga
     2341 gaagtcgaat attcacaaca aacctacatt ataccgtaac tttactttta gcttctagtc
     2401 actaccctcg acttaatatt tatccaaaat atttatagaa gcgaattaga atactttaga
     2461 ccagactcta acttgcgaat tcgatcgtag atattcgaat gatccgccgc tnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ctacttcagt agttacataa
     2581 agaagaaggt tatctccaaa cggcatcgtt atgcgtttaa ttttttcata ttctgccaat
     2641 acgtacctac cctttcctat cttgtctgca agttcactcc gaattttcca tgaggtcata
     2701 gcaagctcta gtgatttttt gctttcttcc ttggttagta gattttttac accttcatgg
     2761 tgacgagaat acattatctg accatttgca tcacaaatcg tcactaacct aatctttgga
     2821 tcgatattta ttatatctac ataatgctgt tttattttaa agactctaat ccttagcaaa
     2881 gagctcatta taaacccaaa tattgccccc cagatcatat ggaatacaat tgcggtaaga
     2941 gatatgttta tgactgcctg gcttagttgc tgagataata tcattttttg cgatgaaacc
     3001 gctagaatga caacaacatg ctggatggaa ggttgtatta aaagtactgt tgttggtagg
     3061 aataaaacta accatatggc aagacccgta cctataccga ccaagacact cttgtatgga
     3121 ttaagcatac gaattttctt ccatctaatc ccaactgccc caagcactgc tcctaatgca
     3181 gtcccaacta gtaaatgaaa gccaaaacct aatggggctg cgattatgac attgttcaat
     3241 ccgaaactaa tacctataat cgaataaaat gtacctattt gcaaacccaa tgccagttcg
     3301 gatgccgcaa tagctgatga aatggaccaa gttgcaataa gaccggcaaa tgctccgtac
     3361 tttgcacctt tcaatgtcac tactaagtca tttttacttt gatggttatt ggataatgtt
     3421 aaggtccccc ctcttgccca ataccagtga tggtcctgga tattataaag agttcggtgg
     3481 atgatttgaa tcagtatgat agctgtaaaa taatgcgaga atgcatagaa tttcgctaaa
     3541 gcttaaaccc cataatctac ttcgaggtta ggggaatata ccagttaggt ttgtgcaatt
     3601 gaaattacga aatcagagaa tttcgaaatc tagttacacg tcagcaatta tcaaatacat
     3661 tagttcgaat gctgacgcaa catcttaatc cgttggctag ctagcatcaa cgctattgtt
     3721 aggcgtgcag acaagccctt gctgccgtaa taacacgctt atatacggat aggttacatg
     3781 gtcgcctcgc caaacctcta gtagtgttac agatcctgct atcgtcgtgg atgtttgttc
     3841 gcacatattt tctggtacca ttggaaaatg tgaaaacatt attttgtgac atccaaaaat
     3901 atattgtatt ggataacagc agtaacacct ggactctcca tgcggagatc agtatcatac
     3961 caattggaac gagcagcaca agtataagca aagaagagca gacccttttg tcaaacaaaa
     4021 gctattaaaa ctaccaaact cagatcgagg cgaacaatat gagaaccgtt ctaaatgcaa
     4081 tcgatgccgc ccgccaggca gtcaaatcac ctggtgctaa acgactaata tctaccacac
     4141 gtattgctga aaggatagat gcatcaaaaa cattggaaga cgaagtagaa tccgtaaacg
     4201 aaaactttgg tcaggaactt agagtgttgt agatattagt catacaagaa gagacacgga
     4261 tggggaaagg tcgtgtccca tattgatcgt agccacacac tcttcttctt caacctctta
     4321 tcgtatttgc ttttcctgtt cttccgtttc ttctctatgt cctcttccca aatttttatt
     4381 cgtcagctgt actatcaata tcattttggt agtatatcgc taatggagac ttttgttata
     4441 ttaattagta tcttaactat tgcaacttaa tatatttgag aaaagatgaa atttattttt
     4501 gtgtatgaac tacaagatat ttggcaattg atctagtctt gatatttata ctaagacgat
     4561 ttcatttgat ctttgtaatc aacttcagca ataaagtctc gaatgatttt tagggcattt
     4621 atttttgcat taccggggtt gaatgataat gttatacatc tagtgttata ctggtaccgt
     4681 tagatattcg tcaatattgt aatagaaata tagaaacggc tcagagctag actgaagcca
     4741 tcgttgccgt ttcttggtag tagcatttaa ctttagtcag tggttaaaga taaaccttaa
     4801 gttgggatga ccggttaggt attagaaata gcgttttaac tgtggtgctg ctctcctaat
     4861 ttcagcaata ttggctccct atttagctgg gactattaca cgtcagaaca ttattctaaa
     4921 tgccgtatcg attagtcctt ctgccgcagc aaatgtaaat acccgacaaa tcgatgtaaa
     4981 ccgtcttaca taccatcttt ggtaatgtcc ttagagtcaa atggtaatga taatcccggt
     5041 caaacaatcc gaattcataa cggaatataa agaacatttc ttaactacct tgaaaacaga
     5101 tcttgttgga aaatacaccc atctcgatca ctaaactagg aaacatgcct gtcggtatta
     5161 atattgagac aagttccaga ctgcaagcca acgggctggc gaggaagtct ggtgtcaagg
     5221 tacgtcccgc gctcaagtaa cacctacaca taacaacgca ttagccacag agtcgacaca
     5281 aattatattt aggaattcaa tactattcaa gtatgcgtct aaagctcata tatgccgccg
     5341 gtgggggaat cgttgctatc gccatagcga tatttattat tagcagtact ggaactcatt
     5401 ttaataataa tgctggccag acgaacacta ctgctgtgcc gcttacactg tctataaaag
     5461 acatcgttcc caaccaaatt aataatcatt cagcaactat acaagtttct tttaacgcaa
     5521 ccaatcctaa tagaggaact gcaattttag aggcaattgc atatgatata ctggttgatg
     5581 gcaagcgagt cgtgtcagga accataggaa caaggttgga gggatttgtt acatcttctg
     5641 ctggaatata cccgattgtt ggtgacggat ctgtcatctt aaaagataat caattatttc
     5701 aaaagaataa ttctacagca aatgcttgga acaaagtagt tgagggaaag gcaaattatg
     5761 tagtaactgg atctatgtcg tatagggaaa tatctagtct tcaagcaagt tcgaccgata
     5821 ggaactttag gctagcgttc cactgaataa ccagcctaca tggtattctt cccaaagcga
     5881 tgtgaaaggc tcccaaaccc aacgcttcat cagaagatct cttggtatca tcttttattt
     5941 caacaacaaa ggacccttct ctatccacct ctaaattact gaccttgtaa acctagttgg
     6001 cttgtttaac attaaatagg cttccttatg tctcaggatt ttggttttta caccaatttc
     6061 ttttatcaca gctattagtg actgaattgg tcataatttc acttgaagag aatgcagcag
     6121 ccgtcgaaat attaagattt caactctagc tgccattcca atttctagtt acaaattaat
     6181 gtatcttaac aggccctcga atggccttca taatacctgg tctagtcctg tttctttatc
     6241 aatctctctt ttttcttgtt cagggattgc tctattccag aaaaggtcct aaatagatac
     6301 tacggtaaac ttactagaag actcagccgt agactagtcg gtaatgtaca aattgatata
     6361 tactcaagaa ctatacaata gagcatctta tggccgcgtc cccacgagcg aaaaagcaaa
     6421 tgaaatcaga cccaataaaa aaagagccga taggctcccg gtctaattac gcttccactg
     6481 cggttaggtt aacgaatagt cagaaaattg ctatacaatg gcgcaaaatc tatgatttgc
     6541 ctccattaca taccaaaccg agacatgacg ccgtaaagga tttgtgcgga atgttaaaag
     6601 agtatgctga tcacgaagac ccagtagagt ttgtcaacca aattagagaa aacatatatt
     6661 gattcaaatg tctggttttc gtatgttacg aaaggcactt atgaaaaaac ttatgataaa
     6721 gcaagaaata tcatagatgg gataatcgca aatcctcagt ctgtcgttgt aattagtgag
     6781 ttggtcatac ttgagatggt ctctattctc aggagcaaag tagttcagaa agagtctttt
     6841 acggattcaa taaagagtgg gagtcgagta gcagataggt tgacgtcgaa aatagatctg
     6901 tacacaaaag aatttcttgc aaaaataact gaatctacta tggcaaaaaa actcgttgct
     6961 gtggaatcaa atatcccggc gaatgaattc ttcaggaggg ttgtgcagat acagcggaag
     7021 acatttggaa aaattttaga ccagaatttc tgtcctattt gcagacgtcc gtataaaagt
     7081 tatcggtata ggggagcaga ttacaatgat atacaacatg cgattatggc tcagtttgcg
     7141 agagttaatc gtttaatcac atttgatcgt ggctacgaat ttctgaagcc tttctttaac
     7201 agcacatttg agatacagat tattagatga atcaattttt aacacgtaaa aatacgagcc
     7261 gagaaccata tcacaattca atgcattgcc cccagtactc gttcctttga ttcttatgag
     7321 gttgtcacaa caggcagaaa cgcgcagatt gatattgact ggaaccagct aataatagcg
     7381 cacttcagat gaacactcca ctcatgcagt gagccttatg aatcgctttc caaaacggcg
     7441 taccattacc tcgggtcata aatggtcttg atttgtttgt gaacctcata ggaggtactc
     7501 cgagctcaca acgtaacagt cagcggcagc agaaagatca gattaatagg ggaaacattt
     7561 cggttgcaca agacaaggtt aagaagatgt gttgtccaac tattcgaata gtcgcttaga
     7621 tcgggcctta ttccttttat ctaattgtct gatatgcatt tttgatttga gatttcgtat
     7681 tttgtaattc ttgcatggtt ctaacaatca ggatcgtagc tgacttattt cggcatccgt
     7741 ttagaaatta tcagggcatc atttttagtt gtatatccta tgctcgaaag attagcgatt
     7801 tagcgtaaac attcctatgc cgcctaaatt ctatagcgtg atcttgaact catggaacta
     7861 cccgtcgaag ttcatgttga gcatggagat aaacaaaaat atttcatcaa gaagatttat
     7921 gatacagttg atgaagcaat aattgacgct aaagaataga ctgtagatac gtgagcacat
     7981 ttattgttta ttagtttctc acgctgccaa cattctaggc ataaaaatcc ttgtccaaat
     8041 tatattttgg aattgaatga tattcattca tgcgcctaaa gtcatgaact actgaatatg
     8101 gaaatgatat atcatatcca gcagcttttc acatcgcttt cacattgaac tataacacct
     8161 cgatcgattg ttcactaaat ccctttttaa ttagcaactg tttaacagcc tgcctgtgat
     8221 ctccttgcag tataattcgt ccttctttat aggtacctcc agtacctatt ttggtcttta
     8281 gctcgctagt tatggctatt atctccgggt ctttcatact gtgcgccata cctcttatta
     8341 ccgtagaggt tttcttgtac ctgcctctga tttcctttga tataactatt ttggctgttt
     8401 ccttatctag ctcatctatt atatccttaa ttgaatcttg actgtattga tttgacactc
     8461 atatttataa tgcgattcaa gttgaaaagc attgtggatg gtttatggtg ttattcatcc
     8521 agaacctttt tcaaggattt tgctgaagat cagggtgcaa ttcaaggcgt agcacgcaat
     8581 cttgagtatt ttctaaaggt aacacataca gaaaaaatca ttcgcattct ctatgaaaat
     8641 gtagcgattt atgactaagg ttatcttcgt gatattcgag gacagcccta ggcgttggat
     8701 agaccccaac catcagtatc aatgacaagc cgatagctgt taactatatg aacataagca
     8761 tggccacaag gtccgtagga ctttgaaccc gctgatattg ctcctagcac tagcattgct
     8821 atgtaccagc taagcgagcg agcccctagt ttcattcttg ataagcttga tgtatcaaaa
     8881 acttattgct cctgctagcg gttagcctac attgagtgtg aagaaagaaa agaggattac
     8941 tataggatta gtattagtca tcctagaaga taggaaaccg atctgttgat tctgactgat
     9001 gtcacctagt atcagcttaa gacgtcgtta cctgttgggc ggtatgggtc aaaccaccac
     9061 tactgaggat tgctaccttt gcttcccccg ctgattcctc taatgcaccc tattgatgta
     9121 ctcaaaggtc tccctctcct ggtgcatagg tgatgtcgac aaaaggacag agttctgatt
     9181 gacgttgcgg caaaacttgg ccttgttcaa tttagaaata aaaatcttga atttaattac
     9241 tacttcggta atggcttggt ttgagacttc tatgttatgg cagnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtttgagact
     9481 tctatgttat ggttgcccgt tacccctttt ctgtccgcga aacattcttg tcaaaaccaa
     9541 tacgttagac gaatacacca taagattaaa aaaggttgtt taatgataag caagacatag
     9601 atgctgtaag atttgacatg acatgactgt ttacatagtt gtcgttatcg cgttgttcgc
     9661 attattagtc ttgggctttt tttcgtccga gagtatctac cttacgcaag ctcagagtca
     9721 gacactaaaa aatgacagag tagtaatcct aacctttggt gatggctgga aaacccaata
     9781 tacgagtgcc aagccaattt tggacatgta tggctttaaa ggaagcttct tcataacctg
     9841 caccttggta ggattgccgt tacgtatgag ttggcaggat atagtttcgc tatataacga
     9901 tgggaacgat attgggtcga agaccatgac atataagact ttgaccaaaa tctcttccag
     9961 tggtctcaat tatgaagtag gtcagtcaaa acaatgtcta cgtgatcatg gggttaatac
    10021 cacacttttc gccactccga gaggattagg aggggataat gccactgtaa taaatgaaat
    10081 tgcaaagtat tatgactttg ctattaatgg cttctcgaat cttatgtacc tcaaatgtga
    10141 tggttggaag cgctattcaa gtcagacaga ttgtagaaca tattttgaca atggcacttt
    10201 aacttatgcc gctagatatt ctgttagaga atggagccat aattcagttg acctcgccta
    10261 tatgcacaat gatacgtcta tctttaaaat atttgttcag gaagttaata gccagaataa
    10321 gtacaataat ggcggaatta ttaatgcaat tccgattata gcctatcata gtgtacaaaa
    10381 cagcaattta cgtgatagca ctggtatcgc actattttcg caagaaatga aatatttgca
    10441 cgataataat tttagagtaa tccgtgtatc agatctagga tacaatcgaa ctactaataa
    10501 tctatacata aaaggcttat aagtgaatat tggtcttttt ttttgataca aaatggcgac
    10561 atcccattct gggaacatcc gtacgtaatt acgcgttcct agctcaatgg atttcaacat
    10621 atctgggttc tgtcgagatc gacagactag aggagttaat tgatgatgta ctctacagtt
    10681 ttaatgcaca agttgcgagg cgcgacttat cgctcatgat ttcatttgac atcttatttt
    10741 acgggttaga tgctgcactc tagacttgtt ggcacgtctg gcactttctg caggaaccca
    10801 gaaagtactg atccaaaagt tcctaacaga acgaacatgg ccagagctag cggtggatct
    10861 cccgatcatt acttcacagc tagaacaacg tcgggagtat cttcagtagc tggctgtcaa
    10921 cagatggaat agtcaagcgc attaaactcg tggtgacagc ggtggcagta gtag
//
LOCUS       DAOR01000103            6540 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_47278, whole genome shotgun sequence.
ACCESSION   DAOR01000103 DAOR01000000
VERSION     DAOR01000103.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_47278"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    190..507
                     /estimated_length=318
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    848..1123
                     /estimated_length=276
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6052..6382
                     /estimated_length=331
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1677 a         1172 c          979 g         1787 t
ORIGIN      
        1 tacttgtgtt ccagaaccag ctgcgattcc tattgataaa tcaagtttac ctcttcttgc
       61 aagcagaatt gcgctactat gttctgcagc atttcctact atacctacga taattgcgcc
      121 aacgaacact tcaccaaatc caaatttttt aattgtcact tctacagagc taaccaatac
      181 ttcgcttacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa ggaattgcaa ataagtgttt atgagtaaca
      541 aatgtaaata ttatgctggc tatgtatact cctagtaata caaatgcaag aacatcgcta
      601 atgaattgaa tgttcccttg ccctactaaa cttacttctg ttgttggatt tgatacagta
      661 atggataaaa tagtaggaac tgccaatccg attattgcta agaacaacat tgaagactgg
      721 tagcttgtat tctccttatt gaaaaattgt tctttctgct ttagaccccc tacaacaaga
      781 ctcaatccaa aaattaagag tatattgcca ataatagaac ctataatgga tgcttttact
      841 agatttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctatta actttgcaag
     1141 gggaatcaac gcaatagctg caaaaatgaa aattaagagg ggttcagaat gagtaaattc
     1201 taatactaat gccattggtg caaagagaac aagaaagtat aatacactgg attttgataa
     1261 ttttccatga atatcactat tgtcaaaatc caattacgat tcttcccaaa atagctgaat
     1321 gtaacattat tttatctctt tgttttgatc aatcctgtgt cattactgat agggtttcta
     1381 tttcaatcca aacactgcct gtgtgggctc cggctacggg ttttgtgcat tttctggcat
     1441 attggccgaa ctcagtagtc aattattttt gggagacctt tcctttatgt atcttaaaaa
     1501 tcgttgtcat gccacttaag acttgcgtaa ccgcttgaat gttgctatct ggattcgccc
     1561 tggaatacta ctggaggtaa ctcgagaacg agaggagatg cagttccaaa ctctagtaca
     1621 ttctagatat ttttcaatta gattgatatt tgtttatgct aatacataac tcacaggggt
     1681 cactatgtaa gaatagacgt ctcgctcaca ttgattatgt taagtttatg accctacgcg
     1741 cagcggtatc gtcagttaaa ctaataacat gataaggcat ttatgaatca tagtttcgag
     1801 cagatcacaa acgggattaa attatggttc tatcaagtct acgggacata tttttactgt
     1861 aaaaggtcgg gacaatctac tacgattaaa tctctagtaa attttgttcg taatcacatt
     1921 tgccagcaaa atagccatct tctatctcaa caatcgttgt tttcaatccc ctcttgagtt
     1981 cattatcaaa tttatttctt attctcccgt gaatagcttt tgtatactcg aaacaatatg
     2041 tggctccaag gattcaatta ttctgttcaa acatttcagg ccgtcttcac tgccacgacc
     2101 caaggctcca gcttcacttc tatactcaaa aacattgtcc tcgtacggtg ctatcacaaa
     2161 cagattaggc tcactgttag cttgtttatg aaaatgttcg taaatgttgt ctaaacgata
     2221 attgcgattg catgaccagc atttatcgcg gttcaagtcc cgcactaaac gtatgttaat
     2281 acgtcggttt ctactgtgtt taattgttcg cgtaaataat cttttgcttg taaaacgtgt
     2341 gttggctgac ttttagtcag tagcacgcat aaatgcgatg tcccgctttt tgacggggtc
     2401 gagcgtggcc cgaagacttg acagaaccta aattatcaat atttatctag ctctggattc
     2461 ctggtatgga ctagttatgc cagaagttag taagagtaat ctacacccta aagatatcga
     2521 tgttacaata tccgaaattc atgggatttg tgcaagacca tggaattaca tttaatcaat
     2581 ttttaattga tgatgaacgt taccttattc acacaggacc tgttggaatg tatgaaaaaa
     2641 tagaagagaa agtaaaagag gttatcccat tagaaaaatt aacacacgtc gcattcttac
     2701 actttgaaag cgatgaatgg ggaggaatgg aatttctcaa gtgtccaaag gcaagactag
     2761 tttgcagcga tcttacctcg aagttaaacc tgactggatg gaataatgtt cctgtggatc
     2821 atatttcatt ctgggataat gaaactctga aaacaggaaa aaggacacta agatttctga
     2881 tgactccgca tgttcatcat tgggatagca tgatgatttt tgaagacatt accaaatcac
     2941 ttttcccatc tgacttgttc gcacaacctg gagtaaacaa gcccgtagtt tctgatgatt
     3001 tgtcagatac aatgatttct ctctatagat cttcggggat ctttgctagt gagggacctg
     3061 taagaaaaac tactgaaaga cttgtaaaac ttttgccaaa catgatattc ccaatgcacg
     3121 gttcttgtat tgatgattct atgttttcaa aatatacaga agcgacaatg aaaaatgaat
     3181 ttgcatattc tggaatgctt ctgggacaaa agttggaagg acagaaagtg gaatccgtca
     3241 gttaaaccag gacttagaca attcttacct tcaaatgttt cccgactcta cctcggaaaa
     3301 actcgtctta agcgacgtct caagattaat gcttcgctaa gtccagttag ggagtccggt
     3361 taaggaatca tttcatatcc tttcttgata cagacaatgt cttctcgttg ctactgccgt
     3421 gctcaacacc atttatccct cactccgttt gtagatgcga ttgtcttagc tgcacaccca
     3481 caattggcaa gagacatggc atatctaaga ggcccgagca aacaaaattt ggaaactaga
     3541 tagcttaaaa gatactttga atatgacaaa tctatgactc cataaatgct acactaatgc
     3601 cacagtagta gatgatgtta ttaggtttgt aaaagagaaa tcactttccg atcagaataa
     3661 ataagtattc atagtaaaga agaaaggcaa tactaagatt ttatactttt aacaaactaa
     3721 agattcactg tgattacaat cgatgattta tgggccgctc cattcaaaag cgatatgctt
     3781 gcttgatcta ccttttcttt tccatttaaa ccccatcgca tcggcttcgt ctgctaaaga
     3841 tttcccccat gttgcaactg ctcccggtcc tacttcactt ccaggtgggt taatagtttg
     3901 aactcttttg ccaggtggag tcatgggtcc agttaatgct tcagctcttg caacaatctt
     3961 tcctggtatc tctgcactcc agtaggctaa gtcatctgca acttcaaact tgatcggtgc
     4021 atattcaacc ccgcggacct ctcctataaa cttcgcaaat tcggcgataa aaccaccagc
     4081 ctttccactg aaaatcatat tcagagcttc tcgctgctgt tcattggctt tctcatcgaa
     4141 aaagagagca agactcactt ttgttgaccc atcgcctgcc caaagattac ctttgaaact
     4201 tcccaaggct agtacgttca agctgccaag ggaaacataa ccatagtttc cttttttgat
     4261 atgatatgcc aaaacaccat cacagtcttc aaaagtcgga gcctgggcaa actcacaagg
     4321 acatggtata ttgcatttgc aaacatcaaa ccagtctcca gaaaccttcc actttggaac
     4381 gttattttta ctggtagtag ctactgttgc agtcgttgct gccatatcac ttaataatag
     4441 agctaatatt ttagcgttac atcaatcatt tttgccttgc tttgagctac ctagtctcta
     4501 attttgtgtc tccgtggcgc ctcctttgat tacgttaaca taagttaaca agctgtcaga
     4561 aattgagcgt gaagagcgat tttattttta cataattttt agctattagc taattgctag
     4621 tgtcacttta tagcgctatt gacactcaat gcctttcttt gcgttttcta ataattcttc
     4681 catcgtttca ccagaaatag tatgtgtgca tacgggttca caggcatctt tgtagtttag
     4741 gcttcggacc attaaataca gtagttttat ctcgggagtt aaggacttcg ttatttaagg
     4801 ttaattggat atcttacagc aggtgatgat agcttgctaa ctcatttagc tgttccataa
     4861 acagtcccgt tgattagcgt caccattaca tgctacaagc agaatttgaa agtcgttact
     4921 aaagcaacga caactaataa tgcagctact tttctatcga tatttgttcc attgtttacg
     4981 tctatatctg cgctcacaat taaatggtca aatggctttc gctactatcg ttactaactg
     5041 tgataaagct cattcccttc atattgctca tcttattatc attggatttg ctatccttta
     5101 tatcctcact tctaatgccg ctaccgctgc tactgctact accctgcatg ttattaacgg
     5161 tgacattatt ctgtagagtg attattccta ccatcgtaaa taatccggta atgacaaatc
     5221 ccgtacctgt tattcttgca acccaaatac cataccgtga ccaaattttc tctccaaaaa
     5281 ttattgcagc aaacagtccc atccatagca agttcatcca tcctaaagca accatcaaga
     5341 gaaaatatgg ccaacagcat ccgaggcagt agaggccatg atatacaccc atcctgactg
     5401 ctcccaatac cccgtttcgc catcttctca taaagaaact cattggagat tcacagtacc
     5461 cgagacactt tgtctttaat ggactgaatt gatataggcc agacaaaatt aatacaactc
     5521 caaaaattct atcaaccaat tgctgttgtt ttccatttcc acccaaaagt gaattcatag
     5581 gaatcgacca tcctagaaga agtgcaactc ctgtcaatgc ccatactaca aggtagcagg
     5641 ttacaaacac agtcagttta agcgaaacag attttttatt attatatttc ggtttttctt
     5701 ctgccgtcaa attttcatct gtttgttttt gcctttgcgt agtattatcc cctccaccaa
     5761 tgagtcgatt atacagcaac gccataggtg ttatagccgg aagcatcatc gcagccatgc
     5821 caactgtcca gcttgcagtg aataaagaaa ttgctactgg atcgtatagc atcattgccc
     5881 tcatcatgac attattatta tcagcatcag attgtctact aagcaacaac cacgaccctg
     5941 ccgatgttaa aattagaaaa ataagaataa tcttttggac tttgttcaac tgtaatacca
     6001 tggtaatctt gccattcctt taaggtgtga agatagactt taccatcatg annnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nntgaagata gactttacca tcatgacatt tatatccaca
     6421 gattgacgtt caagaacata ttttatagta tttgcaattg cgaagggccc ttttgtttta
     6481 gcttggcctg aaaatatgat agtcggggct tgtctttgat tatcgtttgc acttttcgta
//
LOCUS       DAOR01000104            4249 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_49513, whole genome shotgun sequence.
ACCESSION   DAOR01000104 DAOR01000000
VERSION     DAOR01000104.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_49513"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1265 a          912 c          888 g         1184 t
ORIGIN      
        1 aaagcagatg gtcttctaat tgcatgaaga gaagatgaag agattattaa ttgcataaat
       61 aataatctct tgactatcat tctaaaacaa ataatagttc ccataaattg ggagctttaa
      121 agacatgata ctaaagacaa acagccatga gatctcccca tgaactttta tttttgtcac
      181 taacacaatg aatactagga agaacattag gctgactgaa aattctcact tgctgctata
      241 tttgcaccat ttgaatatcc tagagctacg gttggaggtt ttaaagaact tgtacaatag
      301 ataaaagaga ttgtaggtac tcactcaccg acaaaggtat ggctttagaa ccaatcttgg
      361 cgcacatagc tactttctct acaaggtacg aatctaaacg tgtgtttaaa gatggaagac
      421 caaggatgac aattaaacaa attttcagag ctgatcgctt atcagaaatc tatgactatt
      481 gataatacta tttatttaga attatttctg aaacttaggc gtggcctacc gcatactatc
      541 tgcaacatca accgaatatt cattatactg gaatgagaac tttatcatct aacatgaatt
      601 ccccatactg gggtcatcaa ctttaactac tattctaaca taaataacgt catggcatca
      661 gagagggaac taaaaggtta tgtttctaat ccatttctaa atcctgtagc cttctggcaa
      721 aactatatga taaattggat tgaagcgagc agaggatttt atgaaaatgc aatcaaagct
      781 aatgagcatt ggctcaaagc attctggggt ccttcgttaa gggcagcggg cctagagcga
      841 aaagaaacag caaaagtaga atagtctatc acctatcaga accggaacaa tacatgtcaa
      901 gaaataatcg aaaaaattat caggcgttgc cagccgacga gccgctgggc gcattggagc
      961 cggttgctta ctttaatggt gcgatgccta ccggcgtgac cgtgtcgcaa caaggacgca
     1021 tcttcgtcaa ctttccgaag tggggcgacg acgtaccatt cacggtcggc gaaattcgtg
     1081 gcggcgaagc gcgagcctat ccgaatcagg cgataaatga gactgacacg aacgatcctg
     1141 cggctacact cgtgtcggtg cagtctgtag tagtcgatcc cgcagaccgc ctgtggattc
     1201 tcgatacggg cagccctctg ttccagccga cccagtatgg tgggccgaag ctggtctgcg
     1261 tcgatcttag aacggatcag gtcgtcaaga tgattctctt ctcacaggag gtagctctgc
     1321 caaccactta cctcaacgac gtccgttttg atctacggcg cgacaatgaa ggtatggcat
     1381 tcatcactga ctcgtcgcag aagggaccga acggaatcat cgtcgttgat ttagcgtccg
     1441 gtgacagctg gcgcagactc cacgaccatc cctcgacgag ggcagaggat ctgtaaacct
     1501 ttctcccggt tgttgaagga cgaccgttcc tggagcgtca gccgaacgga tcgataaagg
     1561 cgggtgctag tatgggcgcg gacggtattg cgatcagctc agatggaacg cgtctatact
     1621 attgcccact tggtagccgt aaactctaca gtgtggaaac agaagctctt gcgaaccggt
     1681 cgcgcgcgga ccgagacgtc agcgccaccg tgactgacga aggtgacagg ggcggcgcat
     1741 cagacgggct tgaatcagac gcggccggga atatctactc gactaactat gaacacaatg
     1801 cgatccttcg ccgacgtcca gatagggagt gggagacagt agtgcacgat ccacgcttgt
     1861 tgtggccgga cacgctttcg ctcgcagctg atgggtatct atacgtcaca gctaaccagt
     1921 tgcaccgcca ggcacggtac caccaaggac aagacctgcg ccaaaaacca tacactctgt
     1981 ttcgagtccg gatcgacgtt cagcctatcc tgctgcgata agcttccagc acccattcag
     2041 ggctatcgta tggtacgcca ttttttaaat aaattcaaat ggcgtcagcg ttaattcaaa
     2101 atgctataat ccttctccca tagcgagtac taacagcagt gaagtacatg taataccgcc
     2161 gaaaattgta atgaactatg ctagaaaaga gtatcaaggt gaattatctg attctaaatt
     2221 cagaggagaa ataattagtg agcttcatat tccccacaaa aaatctaact gcaaatcttc
     2281 tttctaaagt ggtacagata gaaagaaatt cttgtgttca attcgcaatt aaaggaactc
     2341 ctagaatact tccaccgagt agtttagctg taactgcata tactaataac aatggaactg
     2401 cgataaaagt actaggtgcc gtaaattcta acagaacaat atttagattc ataatagaga
     2461 ttcatgacag ccccggctgc ggacagactc gaagagattg cgctcagctg tgatagctga
     2521 gagtattgcc aatacactag gagcaaggaa tatagttgtc gagacagtat tcataactga
     2581 ctttaaattc tcacaatcat ttgctgctca ggtagaatca aaagtagtag cgttccaaac
     2641 gtttctaaca gaacaaaata atctactagc tatacaggta gttgcaaacc agacagttgt
     2701 tcaggctcag gcatcagcaa gagcaaatgt agccagagct agcggtgaat ctcaggcaat
     2761 caggatcatt actgtacagt taaaacaaag tccagagtat cttcagtggc tatcaatcaa
     2821 caggtggaac ggacaaatgc cttatgctct ttccagcacc attttagtat cttcgcacgt
     2881 accggcaatc tgagtgttgt gttcgtactc attagcgaag ggcagcaacg gagaaccaaa
     2941 ttcattttcc agagtgtgcc tgaggtccaa ataggttaag aagatgtttc ttagctaaat
     3001 ctatttgacc ttgcgatatt agtttcaata attcttcgat atcatttcga tctataataa
     3061 caacaccagt atctttggca tgctctatcg atgatgtgca gtccacagaa gaaaatattg
     3121 ttggaataaa ggtactacct attttcaagt taatctgatt tgcggtattt cttattttgt
     3181 caatagcatc atttgtagga acagacgtcg tacaatctac catcaaggag aatccatgac
     3241 tagtgttgta tgctatgata tctgctgcat acctatcttc atagtatatc ttttcatcag
     3301 ttttattaac tccacctaac tgaatgggca gaaattcgag gagagctaat aaccatgctg
     3361 ttgatacctc ttgcttttta ttatcttaag agagcatata atctcgtaaa ttttttcctg
     3421 caccaaaatg agtgaaagct tggggcaatg gaaattttct ctcatcaacg ctaggcgatt
     3481 ccaagatctc tgaacctgta tcatacgaca gtcccaatac ttctaacgac ttatggacga
     3541 cttctacttc tagatgatca tttggcaaca tcgctgaaat cattggcaat gctatcttag
     3601 tcatatcttc agaggaagat tgaatatgtt cttgcaactg gaattctcta cgttctacca
     3661 tgtttccatc gcgaagaatc tttactattg ctaaacatcc tgataataac gatctgtgga
     3721 tactatactt cgctagaaat ttttcttttt catcagatgg tacagcaagt ctcttgcaat
     3781 agactggaaa tatcagtaaa acattatttg gatttctcag ggaatcagga gttgcatcaa
     3841 gccaagagtg gatctcgctt aatcttgtcc cttgttcttc ctcggtaact tggagtatgt
     3901 gacgagtaaa ggattcaggg ttttcaggca tagtcgtgaa taccatttta tggatatatc
     3961 cacatctttg gccagacgta gtgtatagct gatcgttccc tatctccttt tgaaaaatct
     4021 cctgaaagtc agcaccagct agctttgtac gaactccatt aagaatcacc cagccatttt
     4081 ttatttcaga taagatggca tctaaatatt gcacatcagt attttgaaag actactttaa
     4141 atttctcact tgttatgggg cctaatttgc tttgttttat ctcctgactt tgttttagct
     4201 ctgttttgct atcttttgtc aaaagcactt ttagcacaat actatacct
//
LOCUS       DAOR01000105            4423 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29813, whole genome shotgun sequence.
ACCESSION   DAOR01000105 DAOR01000000
VERSION     DAOR01000105.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29813"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2748..2919
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3924..4117
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1248 a          768 c          725 g         1316 t
ORIGIN      
        1 cgttttctag cttgcgacat ttgtttagtt ggacctttgg tttattcgtg ctactataat
       61 ccaacgagct attattgtac gatttacagt atttcttatc ttaagccaga tatcctgaaa
      121 atgttagtaa ctttgaatcc tatattgcta atcaattcaa tagaattgat tcgagcgagt
      181 aaatcttcca gagctctaga gtatactatg atggtataat ctcagctgat cgaccagcta
      241 gtagtaggtc gctctactta tttcagtaga tgcatccatg gcatcataaa ctgtaaacta
      301 atacgttact ccttttttaa gtcccagagt aatttgatga gctcttctac agttgttaca
      361 acataatctg aaaaatttat agaccatggt gtcggtacag gatcttttgc taacaagatt
      421 atctttttac cagaattctt tgcaacaaac atttctatgg cggccccata acttggcata
      481 cttgccaata caactagaac gtctgctttt tttacgtatt ctagatcatg attaactatt
      541 tgctgagaca attccttttt attacggaaa tccttgatat cagagtaatc cataccttta
      601 ccaacaatat gatctactat gtcgaagcct tcagtatcca agaaagtgta aatatgtctt
      661 atgtcatcaa taccgccata acctattgaa ccacaaacca ttatcagcaa taaaatttaa
      721 gatggagaac tctgtaataa tatgatctct gcgttgagag ctatctgttg atgaggatgg
      781 gttttgtccg ggatgtgtcc cagtagtagc gaacccaagc cacagacaaa atagagaaat
      841 gagatatatt ctgagttctc aatgccccta ctaatttatt accgagaaaa aggaattttt
      901 tagcatcaat aaaaatccct gaaatccgat tctatattta tctatgaata catagcgcag
      961 tattaaggct caatcgttac ttttggtctc aaacctgtca gctctcatat tagcttagct
     1021 acatccttat caataccgag atcctcagct aaatcgcttg gatgaatact ttgaagtaaa
     1081 tccgatgtaa aaccatgatt gattatcaga tccttcaaac ctgctggtat atctagttga
     1141 tttataaaac ctatggctgt ctgccgctgt tttatcctct gcaatgtaat atctttcgct
     1201 atcagtttgt gtctcacgct ttgtagggaa gattgaatat ctacttcatt cctattataa
     1261 gcagtactca ttgattacct aaaagtatag ttgttatata attgcattag caatagcgat
     1321 ctaaatagaa tacatacact ttcgtgatct atattcgata attttatgtt ccgacttatt
     1381 ttagttagaa ggtacgcaga taagcggcca taccaggttg agggaggccc cccgacagaa
     1441 gataaatgct gctgttagat aaaaaataat gatgctgtgg ggctatcctt caacttacac
     1501 cagattctat attgagatca attgttattt cgaatgttac agtctaatag gtatcgcgca
     1561 ctaaccatgt cttcttagtt tatcaatgcc tgaatctgaa gaaacaagaa gattgtggtg
     1621 atgacggtaa tcgcagtagc agtaggttta gattttattt gtctctcgcg gtgatgtcgc
     1681 gatgtcttca cattcatgcg tgataaactg cattatatga gatcgccaaa actatagtat
     1741 atattcgcat tcagctcgga aagtaccttc aataaactag atcaagatta atcaagcctt
     1801 gtcattctct ctcttcattt cgttgattaa tctgatttct tctttaatca ttgtatgtat
     1861 ttcttgagtc attttatgct gtgtttgaat tatttcatca agataatggt tttgcctttt
     1921 acctaccatc aatatcaaca aaccgatacc caatgatata ataatattca tctccgtatt
     1981 ttcgatttgt ctaggtatca gatcggtaag gtagaagaac acgacagaag ctcctagcac
     2041 taacaatccc agaagaatta gttgcatttc attcataccg tatctacttt gccggtgtat
     2101 ttacattttt gaacaatcag catcaataga aatccgtcat gagtttacct acctatcgcg
     2161 atagcgccag gaacaggcca gatatgtatt actagacgag cttgacaaac tagatggtga
     2221 tgagggtttc ccattcgtct tcaacattag ggaaggtgtg tgcatgggta acatcttcta
     2281 ttctagaata tttacgacat agccacctga tatactcctt acaaatatta caaactgccg
     2341 taggagtaag atcatcacca catgctctac aagagcgttt gttaggcatt ttctaacctt
     2401 acccatctaa atgatacggg tgaaagaata ttggcctaaa tcttatttgc tctcgaaaat
     2461 tctatctgca atccagcttt ggatcctaca tccaactcgt aaacgtcata acttgtaaga
     2521 ggaattacag aaatgttatc gcttgaacaa attggacaag agcttgtaat gtgcacattt
     2581 ctttctcctg atctaaggat ggttgtactc caaaaacagg attcacaaag tgcaaactga
     2641 tggtctgccg cataagagct tttcactaga gggctaactg gataacacat ttcgttgtta
     2701 acgtagcaat atcttccata aatcaattgc ctaatgaaag gtcatagnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttagtacttc ataatggaaa
     2941 aactttactt cctagattac gagttgaaaa tacaccacac atcactgggt tgagtgttaa
     3001 caatagctga tcacttgctc aagagtcaac ctaaaatgac gctatggagc cgtaccttca
     3061 tctcgctaca atttattttt tgttcgcggt aaatactatt ttgtgtatga tattgcatcc
     3121 atgtatagtt ggagatgaag gagagcagtg tttttcgtta cgttgataca attattttcc
     3181 acgtgtgtga gaacaattct cttcactttc gtagttaaaa ttatgaatgt gcgtaacctg
     3241 cataactata ccatacgtta agctaattcc ttctctggtt attacaattg tgggcttgcc
     3301 cgtattttac atccttgaga agttatcatc acaacgtgga gattggattt caagtgccaa
     3361 ttctatcgtg ttagcgtttc tctctttctt gatattgtaa aacacttgtc gcattgatct
     3421 gagttagtga tcctgtgctt ttgatatgaa atttattcat tgtgtccgaa tgcgagatgt
     3481 tccccatcgt gacacactgg acaatctttg tctttccttc gttccaagtc aaaaacgact
     3541 ccgcaagttc tgcagatccg tatatttttt gccatgctaa tagtatgtgg ttgcttactt
     3601 aagtacactt gccattcatg atatactacc agtgatattt gggatccaat tccaacgacg
     3661 gagatttact cattctcaac aacattagaa tgattaaact tattattgat cggctcctta
     3721 aaatattatg ggagcggtgt tcaggtctaa agaattccca agtggtcgac ggaagttggt
     3781 ggaagaatct ataaaagatc tcagtcctga tatcaaagat aatgtgctaa aggtactgga
     3841 ttcttgggaa ggtgtaagat cgcaagaaaa actaaaggta attttgggtc aagataaggc
     3901 tgaaagctta ttcaagaaga ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg aaagcttatt caagaagatt
     4141 aaggcaagca aaagcgaaat aacttacgat gagcaggaaa aattacaaga tatgttcaaa
     4201 gaatccctaa cctttgattg aacttataac ctaagctata cttacaaaag gatttatgac
     4261 aatatgacaa aagtgagtga gtataggttt caaagcggga agggatcctt ggccaagtgc
     4321 atctgcggaa caaaagaacc accaaaagta gaatagtaac cattgattat ttacactcga
     4381 catctccaca tactaaccag atgatgatta ttggcgagat cta
//
LOCUS       DAOR01000106            3993 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_32710, whole genome shotgun sequence.
ACCESSION   DAOR01000106 DAOR01000000
VERSION     DAOR01000106.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_32710"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1204 a          834 c          716 g         1239 t
ORIGIN      
        1 gatattaagt tcttctggta tttcttctac actcaatatt tatcacgttt ttcctcatcc
       61 ttaacgcctt tgtatacatc caattccttt agaatgttta ttacaagagg gatctccaaa
      121 tcaacctgca tcgcaatgaa ctcttcggct atattggaaa tgtataggtt tttgacaaca
      181 actttctttt cgtaaggaac gtctttggcc gtggcagaga tggtggtcgg gtcagtgtat
      241 gctacaccct ttagcatatg taatctatat ctattcaaag atataagctc atctgaaaaa
      301 agactaaaca tatagcaaac aaatttgcaa ctgacttgac aaattgtaac ctttatgcct
      361 aaaacacgta acaacctatt caaatgtcaa ctgagcctga tgatagtggt atgccataag
      421 atgaacctta aagtgcagca tcgcctgagg cttttgtccc tatgtcataa gattcagcta
      481 tatcagacac aaatatcttg ccatcatacg atgaaccagt acttatagcg tttagcacat
      541 catctataat gcgctttgcc accgtgtttt ctacaattac ttcaatctta gtccgcggac
      601 caagctctgg aatgtaggac cttgtacctc tttcgacatc tacgctttct tgttttgacc
      661 gaccccttcc tttgacatca taacacgtca taccgcctac attatgctta tgaagtactt
      721 cgttaactgc ttcaagacgc tcatgcggaa tcaagagctc caatttcttc atactattga
      781 aaccagcaga gaaactaata agtttctctg acgacacaac cgatagttct ctctgcgatt
      841 agttgttcag catcaaattt atcagctcta accttgactg attcctgaat ggttggcgca
      901 gttattgtgt cggcataatc agctcctagt tgttgttata ctatatttgt tctatcctaa
      961 tgacgtatat gacaaagacc aatttacggt caataagttg tgttaagact gctcaagata
     1021 tcaacgataa ttgatcgatt ctagatatat ctacatacaa gagctactgc agtataagga
     1081 atatctatac tattacgcta tttttctcgt ctttgttcgt ttttttgctg tcagctatct
     1141 catgaaggcg attgtataat tctaataagc gcaacccctt ttctgtagtt ttataagttt
     1201 tcttaagctc atcatgctct aacaagccat ttctcaatag tagccctacg tactcctcta
     1261 attgggaaaa agacagaaac gcctcataca ttatttttgt tttaagaaca ccactcgtcc
     1321 tactagctga cgttaaaata agcgtaatta tttctgttct atctctgtat ttcatatgtg
     1381 ataattacat agtctaaatg actatctact cctttttcac ctcgttacat attcgctata
     1441 tggatataaa aatcaagacc ttggtgattg gaaaatattt aggaatgtga agattttgcc
     1501 agttctagta ctgcctctgt actacctggt caaaccaaca ttgtcgtccc attcctgtgg
     1561 gtactactga atcagcctag ctttatgtgg gtgctatgat atctcttaca ggaatggtct
     1621 taataccatt cgtaccaaca caatcaaaag caagtaattg cacctttggt tttcatagtg
     1681 ggctttttat ccaatgtggt tctggcgcct gttcgtcgtc agttgctggg acttctcaaa
     1741 gtgcaaaagg gtctatagat tccggaagta atcctaattg cgctatggcg ctagctcggc
     1801 tagccataca cgctagtagt accaataccg gcggcagtgg gagtgttagc tgcagtgtcc
     1861 actctccata atttcttatt gcccttttca gtttctaaaa tattcctctg acgatggagc
     1921 gggcaattaa ggaataagaa ttagctttgg acgcgatttt gtcgtctcct tattttattt
     1981 catatcagtc tttatataga aaagaaataa aagttatcga aaccattcaa gtaattttct
     2041 acacgtcgaa attgtttaaa tctaaaagag tttagacggc ttaaatgaaa agagggattg
     2101 gagataggag aattatgaac cacacaggaa caaactatcc ctaatcctct cctccttaag
     2161 ccaaatctac acttgaatta tcattatgta atggtaggat aaatacgatt gctaacttta
     2221 tacattgttc ttaatcggta aaagatatct gtgttgttat gattcctatt ctaggtaata
     2281 cttgaatcaa tattttcacc ataatctttc taatgaattt attatagttc gaatatatcg
     2341 acagattctc gtatattctt agcacttcag gtgaagcact tctgatatac tatagcttgg
     2401 gatagcctct gataaatgag ccacaaaatc gtacatacaa taaaaaacga caaaccaggc
     2461 actgcaatag ttgcaataac agcacttgct gctgtattac tcgtaagcga attatagacg
     2521 ccagttcttg aagtaattca tcgtcttagc gctagataga aacattagat tgaaaagttg
     2581 ctgccccctt agatcaacgg attgcaaaca ccggacaatg tgcttcgtga atcactttgt
     2641 tgacaatgct tccaaaaaaa tattcttcta ctgcactcat acccttagtt ccgatcacta
     2701 taacaagatt tctcggcgta atcaattatc gcttttccaa ctatgccata ttcttccaga
     2761 gcttccccct ttattttcag gccttgtttc tttgccaaaa gatcagatgg ttggatagct
     2821 ccctttttta ttttccgacc ttgggcccga tgtattctgg ttatcacacc tgacaccaga
     2881 ttcatggggt atcaatattc acttgactct gaggtaagat gagaagtgtt tgccacgtaa
     2941 ggcgtaccag cgtggacatg tgtgagcatt agacacacct taaattcaag agatatcttc
     3001 aataggtctc gagaataaat aatggttcga taatgtttaa ttattggact gaagtggata
     3061 tctccttcta ggatgggttt aggaacatgg ttttgcattt tattattatc ggtggtagca
     3121 gcgctgatga tatcctcaaa tgctccatct gttattctag ctagtagttt tcgacaaacc
     3181 aatcagccga tcatcacgca tcaataccag acaacagacg ctaccggcac taggatagta
     3241 ataataccac ttggcgccgc agaacaagat gttgcaaaat attatcagcc agccaacgtt
     3301 actattccaa atgatactgc ggtgacgtgg ataaaccatg acacagcagt acatactgct
     3361 acagctgacg acgcttcctc ttcctttgat acaggcataa tacctgtcgg agcaaatgcg
     3421 actgtgatta taaagagtga acgaggcaat atacaatatc actgcaccat acatccgtgg
     3481 atgaagggca gtcttaccat ctcatctttg acccattatc ctaatttggg tagtagtgcc
     3541 gaacaaaaac aacaatatca ccaacaaaca cagagtaaga ttatcgctgc tgggccacca
     3601 ttaggtcata ctacaggtaa gccaacgacc ctaatattac taccaaaatc tgtaagtaca
     3661 acactgggaa tagaaccaga acacaaaaat gattggataa ctgcaaacca cgatatcttc
     3721 ggaactaggt atagcggtca gacacttatt ggtgaggaca atgtgaacaa gctccaagtc
     3781 aaatggatat tgcttaacaa attcgttatc gaggatccgc ctataattgt aggcaataaa
     3841 ggcttcgtac aggataacgg cggtaatatt gaaggacttg ataccaatac tggtcttata
     3901 ctatggaaga tacgtccagg gactggcggg aatatgcacg gcttaacgta cgatcatgga
     3961 aaattatttt caggaaaagg aaataataca aca
//
LOCUS       DAOR01000107            2716 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_13880, whole genome shotgun sequence.
ACCESSION   DAOR01000107 DAOR01000000
VERSION     DAOR01000107.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_13880"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1383..1585
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          727 a          522 c          512 g          752 t
ORIGIN      
        1 tttcaatggt ccaggaaaag tgaatgccac tcagactaga ctgagtgatt taggagcgcg
       61 gggcgaccag ttgctacgaa tgatacgagt caacccatca aagttgtata tcttgaaatc
      121 ggttaaaccg atagctcacg agataattga aacatttgaa gcacatatta aagacctgag
      181 aagtaaaggg aaccattgtt actactgtgt tattgatggg caagattcgg cagaaattct
      241 ttttgcttac ggctacttgg atgagaattc ttccactatt tgacgcattc agaagagaat
      301 actattggaa ccgttgatca accagtaacg tatattcatg atacctttga tatgttccaa
      361 attcacatgc cttcccggcc tgaatatcgc cgcttgtcag atgatccagg ttttatctac
      421 tttggcggtg gaacgaagca aacctcattg ccacttgttg ggtctcctct tcatctcatt
      481 ggtgtcgctc ccgatgaaaa agctagaatt agttcatcca atttgcctcg cttagtcatg
      541 tacatcaata aacgaatcaa tgagattgta cttggacgaa atccgcgata tgatggactc
      601 ggagcaattt ggcatgctga cacttataac actgaccctc gaattccagt ggagttcttt
      661 gcttatcgca ttcacgatag cggacagcgt tcttttgggc atccagcaca tgaaaagcga
      721 gttctccttt atacgccttt atatgtagca tataaagact aattcaatgc gcattgtatt
      781 tacctctgta agcctattag taacgccgac aaaaaaacca tgtgcgaaat agaagttcgt
      841 ttaacttcct catttactca tagtgaatta taatatcatg caaacaaacg gagatttacg
      901 ttgcctgaaa tttgtaaagt ttagactctt gctttcggaa ggaagccatc atgtcacagc
      961 aaaaaccacc tttttggtac agatttcttc gtccattacg ggatactcct accccgattt
     1021 cttcactttt ggatagtagt actataccag aaatcaagat tatcaccgaa aacgacttaa
     1081 aaatagaaca gattttgatg gaagagttta gatttagggg ggaatgctta aagcaggtag
     1141 caagcgatat cacaagtacg tttaatttgt tttttttatt tataggtata tcaatttcgg
     1201 ggattggcgt tatctatcaa ttagcagggg gtatacgcaa taatttgcaa acaatagagg
     1261 cattaatatt tcttatttta ggagtaacta gtacacggag gctcaaatct gcgtgtggta
     1321 aaataggacg acgacaaggg aaggagaaaa gaagatgggt ggtcctaaac cagccgtgtt
     1381 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnntttct tattttagga gtaactagtt ctctcttttt
     1621 tgtacggttt gctgctttaa tacgaagtca ttcgcgcaat acagcttgta tggatgtgat
     1681 tagggcatat tatattaagc accttcatag ccagatacca gatattagca atgtttttcg
     1741 tatctctatg gtcaatactg attattataa ttcccgtaca atcttatact tcacttttgg
     1801 agtagcggat agcctttgtt tcgcaggagc cgtatttgtg ttcgctgaac ttttgctcgg
     1861 catcagaagt aatacacttc tttttttacc ttccgattta cgtccataca tttttggact
     1921 gttgattggc gcgattgttc tcctcaccca tatccaatta actagaatta ctgtgtatcg
     1981 atatcaaaaa gaacttcaag aaatatttgt aaaagaaaag gcagtgtaat caaaatttac
     2041 actgttcaca ctttctcact accaattttt gacccctcta atgtcgcaga atgaccaaat
     2101 cacgacatga aacaagcagc tctgaggcta gcgactctta caatagtagg ccgagagtgt
     2161 gaaattttgc ggattatggg atagttcaag atttttgaga aatttttcct ctcatcaatt
     2221 gttgcaagaa atgagcgtcg ttgggaaggg aaataaagac cgctgggtgc ttatccccag
     2281 tgcgatttat cgagagttac tggctttaag gcaggatgca ccaattgact ctcctatgtt
     2341 taccaccagc actgaccata ccatggatag atcgcgagta tttcgcatcg tagcggacgc
     2401 agtggtcaat gcaaggatag agcaaaaagt gccaccgcac tggatgcgcc actcacgcgc
     2461 ttaaccacaa cgatcctgcg caattgttgc gcgacacctt gggacactca tccctgctgg
     2521 taaccaatca atattcacac gccaggccga acacatccag ctccacctac ctgccgattt
     2581 gataagcgat aggtggacgc tttaattctt aggcatcata ctctcataat ctaatgtgtt
     2641 tgataatttg tagtttggtc ggtcatatac aatgctccca ccccgacctc ctgcacggtc
     2701 ccaccgaaag catttg
//
LOCUS       DAOR01000108           32573 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_5326, whole genome shotgun sequence.
ACCESSION   DAOR01000108 DAOR01000000
VERSION     DAOR01000108.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 32573)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 32573)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32573
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_5326"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5164..5301
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18252..18450
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21664..21723
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26097..26284
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10157 a         6009 c         6130 g         9690 t
ORIGIN      
        1 taagagacag gattcgcatt ttgtatacct gtgtatgagc gtctctcgta ggtattcatg
       61 catacctatt ttcattgtat catttttttc atgtattaac gttactaata tgcagtaatg
      121 atagccatat gttgttgctg taaaaatatg atgatattac tacacgagca atattgtacc
      181 ttttttctat tttatttgta atagaagatt ttatattcac atctcataat ttcttgctac
      241 ccagcgtaga aataatactc aaaaagcaaa ggctcaattc tgatatttga aatcggatat
      301 tcaagatata tttgctctat tacaaacagg cttagtccat ctagagttcg atatctagat
      361 taatctattt taagtgcgta ttgaggcaat tcttgcaaca atcaccaaaa gaatagaacc
      421 cggttaacgg tgctagaata ttgtactccg ctaactcgtt agcatggatt accgaccgga
      481 cgacttgtac tcggaagcga tgtattcata catcatgatg aaagcgttat ctataatcaa
      541 gagatgtttg ccgctgccaa ttttaaacaa ctatattagc aacatcgcca tcttataata
      601 gtacagacga tgaagatcaa gaagatgaat aactagattg tcgatgctta cgacaaaata
      661 tatagcttgt ggcgacgagt ccacaggact acgcgtttct gatggtagtg gtaatgagtt
      721 taaaacctct gctatagatg ctaacacttg gcatatttgc ataagaggac atcataacga
      781 ctcgagaatg aatatgtcaa ggacaattca tacgtactgg tcttgacctt ttttgaacaa
      841 gtcaaccaag gtagtactgg taacataccc tgatagcttc agattggatg aaggaaaaag
      901 tctagtagag tcatcaggaa caacaataat agacagctat caaattgtaa agatctttac
      961 tcaaaagtat ctgaatcact cagaatatgg tttaggagct ggtaaagctg aagagatcaa
     1021 ggaatttgta aaacaatcca aagttggcaa gattatcgtt gacgaacatt tgaagtcaaa
     1081 acagatacat aacctagaga aactgaccga ggtacctgtg atagacaggg agaggctaat
     1141 actggacata ttttactccc gagccactac gactgaggca aaattgcaaa ttcaactcgc
     1201 ggaaatcaaa tacgaaatgc ctcgtgttcg ggagaatgca aagctgactc caggtagcac
     1261 cgagagagca ggaaaaggtg gtatgggtga atacaccgtt gatgtaaagt ttcgtgatct
     1321 gaaaaggcag atgtcattca tcaaagaaaa gttacaagat gcaagacgaa aacgtaatct
     1381 ttaccttcaa caaagattga aaactggaat gccggttgtt tcactgctag gctacactag
     1441 ctcaggaaag accacattat tcaatcttct cacacatgag caaaaggaaa tatcaagtag
     1501 tctgttcaca acactgtcaa ctacaacaag gtcattaaaa attaacgata caggtttggt
     1561 ggtgttgctt acagatacag ttggctttat tagcagactt cctacgtata tgatagaggc
     1621 attcaaatca acattagaag aatctcttgc tgctgatctg atttttcttt tgatagattg
     1681 ctcagagaaa attgaagata ttagaataaa gtattcaagt tgttggaata ttctagacga
     1741 gctaaatgta gataaatcta aagtttttgt aattttaagc aagtcggaca ataatgtacc
     1801 gcaggaaaga atcaacgaaa ttgcaaatga tttgcagata ttaaatcccc tgattatctc
     1861 atccaagaca ggatatggaa ttcacaaact caagactatg atagcaagta atattgtaaa
     1921 attaacaagt tgacaatcaa taccttgaag caaggagtac gactaatata tgacaggcag
     1981 tccaattcat gatctattat aggtgatgag taatattcgt gactgccgag gagcattgat
     2041 tgcgcagctt ttgttgtggt tacattcgtt gaaagatact gcaacatcta ctgcctcgga
     2101 tatcccaaat atcgagccat agtagaggaa atagttaaat cgctaacgag ttgattcaca
     2161 tattcatgaa gtctatttta gtgctctcga ctgggcttgt atttgtagtg gtgacaacgc
     2221 gcacagaacc tttcaagtca gttggcagta tagatttctt ctagccgcaa gctggtaatg
     2281 tagcagtttg ccagcttagt atacgcatct tatttttaca aattcgtacg tctctagatc
     2341 gatggcttaa tttccaattg cataatacgg atgttaagat catagaatca aatactcttg
     2401 gaggcaatcc agctaaaaaa ctatcatatt cttatcctga ggtcgaggct acacaaataa
     2461 caacaataaa gaataagtct atgatatcac gtaccctctt catttctccg ccaactatac
     2521 aaaatatgat tgattccttt tagatactaa agccaacgat atcggctaaa ccatcgttaa
     2581 ataatactaa tacatccgaa gaaaatcagt tgggttaacc tcagtaatag atcatcgatc
     2641 atacctgtca taccactacc ttgatttagc cgatcttgtt gtcgtctgaa agtacttgtt
     2701 ttggttagtt agttgtttgc gttcattagc agccatccag tcatcatagg atattgagtc
     2761 tgcaatttcg atggccgtag gacgaacagc aactgaaaca taccatacag caccttgttg
     2821 atacagtatt ctattgttgt gatcgagtac ttttactgat attcatctga gttgattgtt
     2881 gtccgaaagt attgctctta accaatctcg atggactgac cactatgata tcggcgttct
     2941 tcaaatatca tctcaagaat tttttccgca ctgtttttgg cttacgcatc ttatgtttgt
     3001 tggttctata ataaccgaac gaaatcttct tgcatcttcg tatctacttc acctggacaa
     3061 attgccatca ctcgtatgct gtaatttccc acttcccatg ccaaactttc ggttaatcct
     3121 atcataccaa acttgcctgc acaatataca gatatatctg gaaatccggt gtttccactt
     3181 ctgaggtgat gctaacatcg catcttacac caattaatct gtcattaaat ctatcatcac
     3241 tagcatcatt atgtttatta tcatctatta taatcatctt tttgatttct ttaactgctg
     3301 aatcaatttc actttgagtt ctagaacata tgactacgtt tgctcccatt tttgataaca
     3361 gaatggcaac ttcttttcca atgctctgct acttcctgta actattgcag tagtcttatt
     3421 tgacaagttt ttctaatcta attatgagtg aatactagac atagaggcta gaaaatgtaa
     3481 tgaataggcc ataacgagta actcacacac actataaaaa tgcgaatagg atacttttac
     3541 aaacccgatg cccggttgct atgctaaaga atggagtata taaagggatc actctacaga
     3601 cttggcatac tcaatctcat ttttatttag gggatcgtaa catcattaaa ggttaaatta
     3661 tccgttgcta tacaaaattt aacacgatta aggtttttaa aattgagcga cctattacat
     3721 gaagaggaga ttggacaatg gagaaggacg cattattcgt cggaaatcaa ctcatcgcta
     3781 gaagataggg aagtaataat tatggggtgg gtttcgagta agcgtgacca tggtaacatc
     3841 cagtttatta tcataagaga caggtacgga gacatccaag ttgtggcgaa gaagagtgaa
     3901 tgttcaaagc gattatttgg gcaaatccaa caaataaaag aacacacttc tcttgctatt
     3961 cgggggacgg ttagacctca agaaaaagca cctaacggcg tggagatcat cccattagag
     4021 ctgaaggcct tttctattgc tagaaaagca gctccattta tgatgcaaag taagacatct
     4081 ataggtattg ataccagatt ggatttgagg gcaatagatc tacgtagaaa tattcttcag
     4141 agtattttcc atattcgtta taccgtattg aatgcaagca gggagttcct agcgagacaa
     4201 gggtttatcg aggtaaacac tccaaaaatg attgctaccg ctacagaagg cggtgcagca
     4261 ttatttccaa tattttatta cgacagagaa gcattcctgg cgcaaagtcc tcaattgtac
     4321 aaggagcaat tgactatggc ttttgaaaat gtgtttgaaa taggcccgat ttttagagcc
     4381 gagccgtcgc gcactaatag acatctttct gaggcaatat ctattgatgt ggaaaaggcg
     4441 tttgtagatt acaacgatgt aatgagtttg cttgagagaa tgatattgca tgttctagat
     4501 tcagttaaag aaaagaacaa aaatgatctt gcttaccttg agtttcaatt gccaactatc
     4561 gaattgccgt tcgctagata ttcttattcc gatcttatag aacagttaca aggcggtgga
     4621 gaacgcattc gatggggtga tgacctctca cctcaggcaa tgaaaaactt gccggatgac
     4681 aggatgaatg gcttttattt tattaccgat tggcctacat ctataaaacc tttttatgta
     4741 aagccaaagt ttatgccgga gtctggtcga gtctctgaat cttttgatct aatgtacgga
     4801 ccgttagaaa tatcgtcagg cagtacaaga gtaaacagga aggacgaatt attggaaaga
     4861 atgaaaaaac agggattaaa tacacaagct tttgattacc atctgaaagt atttgactat
     4921 ggagtgcctc cacacgctgg atttggcgtc ggtctggaac gattgatgat ggctattaca
     4981 aagattgata acatacgaga tgcatctctg ttcccacgcg atatagacag gttaaccccc
     5041 taggatacga gagaaaagta ttaggcagtg gcgtgttaac atgatttcaa aaaatgaaat
     5101 aagacatctt ggttggcttt ccaggttgga attaacggac agtgagctag acagatatgc
     5161 gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn ngagctagac agatatgcgt ctcaaatcga tgaaataatc
     5341 cggtattttg ataaactaga tgctgtttca ttgactggaa gtgagccagc ccgttcaatc
     5401 aaaaaggttt ctgaattaag aaatgacaat attcacgtgt tcgaaggtga tccattggaa
     5461 ttttccaaaa atagaaagga cggctttgta aaaggtccaa ggatgggcta aatgccaaac
     5521 catctatatc gtcttactgc agaccaggtt gcttccggca ttaaaaatcg agacttttct
     5581 gcacaggaat atatatctca gattctagaa agaatagaaa aaatagattt caaaatcaac
     5641 gcttttgtcg ctgtggataa acacggtgcg gctgaaaaag cacgttctat agataaaaga
     5701 attcatgacg gagaatgcac tggtggactt gcaggcgttg cactgggtat taaagacaat
     5761 atatgcacca aaggcttgaa aaccacttgc gcttcaaaaa tgcttgaggg atatgttcca
     5821 ccatatgatg caactgtgat taaaaaattg aaagaaaatg atgcaattat tgttggaaag
     5881 ctaaatctcg atgagtttgc gatgggctct actacggagt tcagcaaata tggacctacg
     5941 cgtaatccat ggaatattaa ttatgtcgcg ggcggctcct ctggtgggag tgcagcgagt
     6001 gtagcagcat tagaatgtgc catgtcatta ggctctgata caggtggatc tgtaaggtgc
     6061 ccagctagct tttgttccgt agtaggatta aaaccaacgt atggatgcgt cagtagatct
     6121 ggactaattt cttactctaa tagtttagag cagattggac ctataggtag gacggtctct
     6181 gatgtagtga tgattatgaa tgcaatcgca ggtacagacg aaaacgatcc gacaacaaga
     6241 gcaacagcca acagtttgcc aacagagaga actcctaata ctatgtcttt cgatagaata
     6301 ccaaaataca gcctgcacca aaacaacagg agtattcgtg ttggactagt aaaagaactt
     6361 gttgaagggg ctgatccggc tgtttcaaaa tgtatctatt ctgcagtgga cttgttttca
     6421 aggtcagatt gtaagtgtga agatacttca atgaagtcga ttgaatatgc tctcgcatcg
     6481 tactatacta ttgctatggc tgaagccagt agtaaccttg caagatatga caatattcgc
     6541 tacgggtttg actccaaccc tgaggagtat gaatggaata catatttcgc aaaggttcgc
     6601 aataattttg gcgaagaagt taaaagacgt attattattg ggtcgtatgt tctttcggcg
     6661 gggtattatg gaaaatatta tctaaaagct caaaaagcaa gagaaatgat aaagcaggaa
     6721 attaaatcac tgtttgaaaa atatgatgtt ttgatagctc ctacaatgcc tatattgcca
     6781 ttcaaatttg gtgaaaagat cgatgatcca ttgaagatgt atcagataga tattgatacg
     6841 gttgttgcta acctcgccgg tataccagca atatcaatac cagttggatt tagtgactgt
     6901 cttcctgtag gattgcaaat aatggcaaat gagatggaag agcaaaccct gtttgatgtt
     6961 gcatcatctt ttgaaagact agttgatatt cacaagagtc ctgagatata attggtggaa
     7021 gtaaagatcg gtcttgaaat ccattgtcag ctgacaggac ttcagagtaa attattttgt
     7081 tcatgccaat gtgattatcg agggagggca ccgaacacaa acatctgccc aatttgttcg
     7141 ggtcttcctg ggactctccc gctcttaaat caaagagcag tagactttgc tagtatgatt
     7201 tcacttgctc taggatgtaa agttccagac aaggtgatgt tttaccgaaa gaattacttc
     7261 tacctcgatc tacccaaaaa ttttcaaata acacaatata atgcgtatgg aatcacaagt
     7321 atcggcgttg aggggagagt cgattatggg atcaagagta caaagattag gagagttcag
     7381 ctagaagagg atccaggaag actaatttac gatactagct tttatactct tatagactat
     7441 aatagagcgg gcgtctcttt agtagaaatt gtcacggatc cagattttgc tgaaccaaaa
     7501 gatgttcgaa tgtttttgaa taaaattact tcgataatcg agcatttggg cgtaggcgat
     7561 acaaagcttg acggttcggt gagatgcgac gtgaatgttt ctttagaagg cggaaaaaga
     7621 gtcgagatca agaatgttgg ctcgtttaaa gatgtagaaa aggcacttgg ctatgagatt
     7681 actaggcaaa aaacaatgtc ggtacgagat atcgagatta aatcagaaac tcgtcattgg
     7741 gatgatgcaa gaaaagtaac caagcagtct agggcaaagg aagaagaaga agattatcga
     7801 tactttcctg agccggacat cccgacaata gttctcggaa acgaattttt atcttcactc
     7861 aaggcaaaaa tgcctgaact tccagatgaa agaaaaattc ggttcctcaa ggaatatgag
     7921 ctttctgatc atgtatctca ggttctgatt gacaataaag agctggccga cttttttgaa
     7981 tcgacagtta aaatctattc ttcatcacca aacgaaattg ccaattggat tgtttctgat
     8041 ttactaggat tcatagatga ttcaaaaagg gaagagggat ctctattctc aggattgaga
     8101 attgaagcga aacatatggc agagttggta aaaatggttg atcagaatat aatcaatagg
     8161 aacattgcaa aaatgatcct tagccagata acaaagactg gagaaatgcc ttcctatgtt
     8221 ctagataaaa tgaatgcctc cctaatagat agcaaagcta caatatcaga ggcaatagaa
     8281 tcaatattta agtctgaaaa atctgccgtg agcgatgcaa aacaaaatcc aaatgccgcg
     8341 aattttttgc ttggaaaagt gatgcaagta actggtggaa gagctgatcc gaagatcgca
     8401 ttgaaattaa taaagactaa acttgcaata atggagtaat tcgtgatttc atattgtgtc
     8461 gatcacctat tctacattta cttagaatag gttatcatac ggatcatacg gacactcaaa
     8521 ataaaatgta caattaacca ttacacatta tttgacgcct agttttccgg tacactgtat
     8581 tggctataag taataggagc gaggttgcca caatatcgat atgtcaagtc atttgatcgc
     8641 tccctttttc tcatcggtga ttgattgctc gatattttct agatgttgga caaccgtggg
     8701 caagaaagca ccaacgtctg ttacgattcc gatagcttgt atcgtccctc gatctatgag
     8761 ttttgtcaca actggctggc ttatatctac agcgacaacc ttgaccgatg aagggagcat
     8821 attccctacc gctatagaat gcagcatggt tgaaaacatc aagaccattt tcgcaccttc
     8881 taatacttgt ttatattttc tctgtgcttc gacaatatct gtaataacat cggggatagg
     8941 accatcatct cttaccgatc ctgcaagtac gaatggaatg tcgttggcaa tgcattcgta
     9001 cataacacca ctctttaaaa tcccctttcg aaccattgct tttaaagatc ctactttgaa
     9061 aacctcatta attgcttgca tatgatttct atggcctctt atcgcaagag ttccatcctt
     9121 gatattcatg cccaaagatg ttcccatcaa agcattctct acatcatgaa ctgcaagagc
     9181 attacctgtt aagagtccct gtacatatcc cattcgaatc aattttgcaa gagcttccga
     9241 tgcacctgaa tggacgatta ccggtccggc taccacgatt atcctgcccc cacgagtttc
     9301 cttagtgtta taaatgtcga ctgcgatctt tcgagcaatt tgttgtgtag gtctttcgct
     9361 ggagctgcta ctgcccataa actgaaaaag gtcaacccct tctcgtgggc gttcctgtgg
     9421 aattatcctg accccttgtt ctcctacgac taccgtattc cccttcttta gatcacgtat
     9481 catcttacat tctgccgttt ttttctcggt atctactgct atgcacttat ccatcatcat
     9541 gtttagaacg tctatccata cgcctttgta gtagacttga gtcgtattgt ttgttgtgct
     9601 gtaaaaatta tcaggcatca ccatgtcttt aagaactggt ttcaacataa cttgttgaat
     9661 ggaaataggc tgtgctcctt ctctgtagag ggattccaat aattggttta gatgttcgcg
     9721 acttttgcct cgaactatga ggcgggcata gctaacatct ttttttctct tcccaacatt
     9781 gaaatccaga acttgaaagt ctcccttcag atccataata ttatcaaaga ttctcgtcag
     9841 gatcatagag tcaataagat gaccttttac ctcgatctct tgctcgtatt ttttttctgt
     9901 caaatatcaa aagaaaacta ttgcatatta tctaattaag cattcgttat attggtagag
     9961 acagcttacc aagatattca atcatattct gctcagcctt aattttagct atgatctcat
    10021 tttttgtatc ttctatgaac cattgagcga tcgattttgc cacgccattt ttgtcgcaaa
    10081 ttgcaatcat gtatccacta gcttccttta caacagcaaa taggttctcc tctcctacgg
    10141 gttcccattt gttgtcctta ttatctttca atgccaatgc gggcatagcg tgaccagtat
    10201 attttgtcaa gacgtcttga gcggctttca cacgtttttc tcctatcttc agagccgtaa
    10261 agtactgcat gcttctatta ttatttgggg cgacgcggct ttaaagcatt ttgtatctgt
    10321 gagataattt cgtgtttgta atccatcacg tattttttag gaaaatccaa aaactgagct
    10381 agcaaatgct cagggtagcc ttcgatgaca tactgtcttg cttgcagcgc catctcaagc
    10441 ttatccactg tatgtactaa tcgcgcaatg tcagttttat ttaagaggta ttccaaccat
    10501 attcctttat aatctgaacg taagcaagga ggaatcgatt gtaaaatagt ccctatcgca
    10561 ttattctcct ctgcgcgttt ctttttttta gtaacttcac caggcacaaa atctccaatg
    10621 attgattcag gcaaatcatg gaggattgcc atcttcataa ccctttccgt gttcaacccc
    10681 agcatatctg ataaagccat gcttattgca cacaccgaat acgagtgatc tgctaccgat
    10741 tctggattac taatttgggc tcttgaaatc catccagctc tttttacatg cttgagccgt
    10801 aagacatgct ggaaaaacgg ggtcagatcc gtcttttttt ttcttcccat tttgtatatt
    10861 gccccgccat tgcacactca catctaagca atatctaata tagctatatc aggtattaaa
    10921 atgtgggaat aagcatatcg aaatagagca atcttagctt tgagagggtt attaataata
    10981 atcaaaataa aactcattcc ttagagctga cttttgttac tagcaatagt tgccaaaagt
    11041 agtcggattt tgaagttaaa gttaccttgt ctgcccctcg ctaggcctcc aagctacgtc
    11101 tttaattccg aggcgtttgt ttatcagcct cgcggctaca aacagaaagt ctgatagtct
    11161 gttgagatat actaagacgg taggatttat tatttggtct tttgagagta atgctgcagc
    11221 aatctcagcc cttctagcaa tagtgcgagt gatatgaaga agtgatgctt caatacttcc
    11281 tcctggaaga ataaagaatg tgattggttc aagttccgtt tcaaatttat ctatcgcatc
    11341 ttctaaagcc gatgccatgt tttcctctgt tctaggagtt ttgtattcat tttgatttcc
    11401 tgcatggtat ctaggatcag ccaaatctga gccaactata aatagttcac tttgaatgca
    11461 cgtcataata tctatgagat ccaagaaaag gtccctatct tttacggtca atgatgagat
    11521 ggctatccca atatttgaat ttagttcgtc cactgaaccg tatgcaacta ttcgtggatc
    11581 tccctttgag atgcgttttc caccgataag accggtttca cctttatccc ctgttttagt
    11641 ataaattttc atacattatc cggtattatg ctggataatt taaaaatatc tgtcagattt
    11701 aatccaatat taataatttt ttaaaactac ttccagtttt tcataaacta tatttatagt
    11761 tccgaaaaag ataccaatct tgaatatgtt gtatattgtg aacaatgaga ctaatacgaa
    11821 tggatctgcg acttgtgttc acgcaggtat tttttcttct taaaccgctc attgcatttt
    11881 tcacacttga atggcttttc gaattccata agaaaccgct cccttttttc ttgtgatgtt
    11941 tcgatgatac tgtcagactt cgcttcagct gtttttatca taaccaatta cgataaaaaa
    12001 atattttttg gtttatttac cttaagtaag atacttacgt taaattcatt aaatatagat
    12061 cgtaattaca tattctggtc ataacgatgg attcacaagc ttttttgaaa cggttctttt
    12121 ccaacctaat atttatgcct ctgcgctctt gtagtgcgga cctttagagt ttctagcacc
    12181 gcacacaaga gaatgactat tatagtttct tctgagttaa tgtctgggag aattgtaccg
    12241 tatactattt tcatatttgc cattttgtct attttttgta atttttatta ggtttctgca
    12301 tattttatgg ttttatactt cagaagattc aaaaaagttt aaaaatcaaa cccatattat
    12361 aactggcagc agaacagaaa acactaaaat accaaatcac tgcagagtct gccataatgc
    12421 ctactgcata tatattagta aactgcactc tcggatccga agaaaagatt atcgccgaaa
    12481 tttcaaaatt accagatatc aaggaggtaa gaggaactta cggcgttcat gatgtcttcg
    12541 tgaaggtaaa atcagataac acagaagcga tgaatcatac tattacgaac aagataagaa
    12601 aaataccagg aataacctca acggtaactt tggtagttat cgacgagcaa ggtggaaaag
    12661 agagcgttta gttttacttt gctagttgtt tctttttatc ctcttccttt atcctcttta
    12721 tctcataaat aattagagca gtagtcgaca gaactaccgc ccaaccaacc gatcctacta
    12781 taaggtaggc ggaatagaga gggattaata gggaaatagc ggcagttact gcccctagcc
    12841 accatataat gattagagtc tgaaggctaa ttcgagtcat atttcttcct tagcctctca
    12901 tatcctcgtc aaagcctttg atgtacctta atgtttttcc acatttggaa catttattgt
    12961 actcattaaa atcgaaatca ctgtacgttc taaaggaaaa tgagtcctca tgactgcaaa
    13021 acgctctacg gattgccctt atgaccatct cagcatttct aggtatagaa gggattcttt
    13081 actctttatt ctttcaaagc caccttgtta ggtaagtatg ccgccggtga gatttgagct
    13141 cacgacagct cggtcttcag cttcactcct gagtgtattt caggtcgagt gctctcccag
    13201 gctgagctac gacggcactt gtttaacgta gttaaacaaa tcatatataa ttgatacttg
    13261 tagtcatgct tgccctcgtt ccttgcaaat ccaaacctac ctttcaactg gagtggtatg
    13321 tcacgtctgc ctcatccgat ccaaagtgag agttaagtat tattttgaga gatttttatt
    13381 gctatgttcc attgattgta aacagcaaca atagcaaaat gcttccagaa tctgagataa
    13441 tggaaaatgt tcatagattt tttgctactg aagttgctct aatcactacg aatagctcaa
    13501 aatacggtcc taatgtgatg gcagtcgaat ggaccatgca gattgcatat gatccaatct
    13561 taattgctat cttcatacat gattctccta cttattggaa tattcaagag acaaatgtgt
    13621 ttgcagtaaa tatggcctca gaagaacaat ctgaacttgt aaacatagct ggtggatatt
    13681 ctggaataga aattcaaaag ctaacaattc caaatacgtt tgacacctat ccttcaaaat
    13741 atattgacgt tccaatgatc aaaaactgta ctgtaaatgc agaatgtaaa gttaaggcaa
    13801 ttcagacatt tgcagatcat atcatggttg tcggtgaggt ggtaagagca acatttgatg
    13861 aaaaggaatt gcctttgata tatacgcgtg gcaactacag gaggctatca agacagaaga
    13921 tcagcggagg aagaaaaaga attgtagtaa gtcattcaca ctttgtagaa ttcaaaacaa
    13981 tgtcaaatgg tcagtttatt ttaaaggcag cagtagccgt tattcgtgat gaaaataata
    14041 aattactgtt cattaccaac caaccatttg gcaacttttt gatgcttcct atggtaagtg
    14101 taaagagagg attaaactac gcagaaacac tccataagta tttggaaact ataggaataa
    14161 ttacagaaat cgagagtata ttgggaattg aaagggttat tctaatgtcc aattatccag
    14221 atggccaaac tgaagataaa aaagaaagca aaagcagtag ggaattaagg gccaatttta
    14281 ttagctttag ttgtaaaatg aagtctatga atgaagaaaa cagatacctt agaaaatggc
    14341 ttgacaaagc tccaaggaac actttgttga gaactatagt taattgcagg ataaggtaat
    14401 gtttaccgca atcagacaat aagttatctt ctcatatcag caaatgtccg caagaacaag
    14461 tatgaaacca atactctcat tatgatctat tcatgtatac ttactaattc agttggacgt
    14521 gcacctaagt tattgcaacc gaatcgaaga acggacttac ttccatgtgt atattacttc
    14581 tatttccact aatatcataa agtccgtcat aaccatctcc ccatcttttc cataatctta
    14641 ttttttcatc accatatatg tgaattgcaa gaacttttct atctccatcc aaatccatta
    14701 agattggagt cacaagcttc tcttctctag tttttgtatc caatagaaaa ttgaatatat
    14761 agtcagcaca tccagtttca tagagttcta ttatactttc gaatctattg tctttgtaat
    14821 tcgatggaaa cctttcaagc tcctttgcaa gctcaatagc taggaaactt cctaaatgca
    14881 atctaaaata tctggccatg tgtgcaattt cttttctgta ggaagaggaa ttttgttcat
    14941 cgaatacgaa ggacgtttct gggtatacac actctcctat cagcgctgca tttgatgccc
    15001 ccatggcata tctattacct agaaatgtaa ctattcttag taatgaattc aggatatcac
    15061 ccttttcttc gagttcaatc atgtcttctg ctactttcaa agggtttccc attgaatgac
    15121 acaatgtttt tgttgaacta gagatgaagg gtctgaacgc gtcagttgat ttccttttca
    15181 gagtatttaa gcgaaggcac atgttattta tctcgtacag atgtttatag gtaaagatat
    15241 tctcaatctt gatactgctc aagcgccgta ctgaggctat aagggatttt acggaatcag
    15301 aggagaattc tgatttctca agaatcaggt ttacgtactc cactagttga tgagtgtcca
    15361 accatgttaa tttggaaaac aacaacaaaa tattataagt atagttttta aacataacca
    15421 cctaacattg tattcctgga atttcggcaa gcataggttt actattgttc taatcgctac
    15481 cctttgaatg gtttttcata aaatcgatag cgttgtatac ttctcttaat agcgttaaca
    15541 ctttttgata gaatcacgct ttgggttaat cggattccag ttccagcttg gaacgcgtta
    15601 attccattgg aatgaacgct ttagggggcc ataattcgta agaaataact caagtgcaat
    15661 attgaatata tttcatcaaa ctaattcgta gaaccagtcc aaagtcaaag acaacaacag
    15721 caattataca cgccagcagc atcgttataa aaactaacta gaaaagactc ctgttgtgat
    15781 atggaccata atcgaataga acccctacct gtttgcttca caataacatg ttgatacagg
    15841 atagcaaaat agatatactt atcctataat ttaatgaatc tgcatggcat catcatcctc
    15901 aacttgtacg aataataata ggaagctcag agtccgtgct tctgatactt gtattctaga
    15961 tgcagcgggt gcattatcat tattgtcgat tgtttattta ttatcaatcg ttaccaacag
    16021 cagtaatggt gatgcgtatg ctcaacaata taatacacca ccacaaccta ctaatcattc
    16081 ctctctaatc caaaaaacgt acaacaatat taccaacaat aatattttat ctgacgcagg
    16141 aacagcaaca actaaagtaa aacctgataa agttacactg atcttaggag tagagacgac
    16201 caaccaaagt gctaaagcag cattgagtac aaattcagct acaatgaaca aagttctcaa
    16261 tgtgttatta tcagcaggtg ttaagcaaaa tgaaacaagc acatctgcat ttagtatatc
    16321 tccaaactac aactattcgc aaggacgaaa cataataaca ggatttacag taactaatac
    16381 catacaaatc gaaagctcaa agattaatga cacagctaga tggattgata ctgcaatatc
    16441 ttctggagga gctacaagtg taagcagtat tgattttaca ttatcagata agaagttaca
    16501 agagatcaaa aatggcttaa taaaacaagc aatcgataac gcaagaagca aagccgacat
    16561 agcagcttct gctctaggat taaaagtgct aggaataaaa tcaatgtatc ttaatgagtt
    16621 tgagcaaccc cctactcccc ctcaaccatt cttacaaaag caatctttag caacaccagc
    16681 tggtgctaac ggaaccccaa tcatttcagg acaacaagac gtgtcagtaa gtgtaagcac
    16741 tgactggtta atgaagcaaa atttcacaaa ataagaagaa atagctataa aaggctattg
    16801 caattcagag taacatttct tgcaaggata tctactcttg actatgacaa gggtagttgg
    16861 ctgacaaata ttgagttggt aacaggcagt aacgataata attggtctac atttagatga
    16921 actctgacct gtttgtttac acctggagta agtttgaccc acgaagtagc tggtgttggg
    16981 ttccggctag gttactgacc gtaaacgggg cttgaatagg tagaaaattt actcccggcc
    17041 acgctacgtt gaaaaacatt gtttcctatt cctacgctcg gaacagtagt aagacattgt
    17101 tgaaccacct ttagcacgta gtatctactg ccgaggatga taacactcta agtatgactt
    17161 tttcactgga aataacagac caaaagtatc acactttgct gcgaatacaa catagcgagg
    17221 caattttggt aaattcaggt aactttaata gcatccagaa ttacttttta acgtgtctat
    17281 acatccggaa aacaactccg attctgaagg gaaaatctca cgtcgcactt ttataaagta
    17341 tatcggcgct gctggcgcag tattcacttt atcttctctg ttacctttca ataaagcgtg
    17401 ctgctggacc aaatgccatt aaaaatacga ataccacaag tagtagtcaa acaagtcctc
    17461 caaaacaaat gacatcacct catacattta atctggatgc taccacgcca caattatcca
    17521 atgccggtgg aagtagaact tttgcaaacg ctgataactt tccagttctc aatggctggg
    17581 caatgtcacc atatctactg cgtcttgaaa agggcggtgt acgtgagcct cattggcatc
    17641 ctaatgctgc cgaattaagc tattgcataa gtggcagagc agcaatgact atatttcttg
    17701 gtagtcctaa ccttgttcat gatacattta ctatagatcc tggagaaatc gtttttgttc
    17761 cccagggtta tttacatgaa tttaacaata ttagtaatga agaggcaaaa tttgttcttg
    17821 tattcaataa cgaacgccct caaaccatgg gcatttcagg atcggttggt tccatgccta
    17881 atcgtgttat gaatgcaacc tttggaatca aaccacctag tacgtttttt aatggattca
    17941 acaaaaactc ccctaacgat atcgtaatag ggtccaaacc agctaacacg gtagaacctg
    18001 cttctatgac aagaatcccg agtattcaca aacttaatct agaaggcatt cctccgcaga
    18061 ttcaaactgc tggaggtaca gttgctctag gaaatattac taattttcct atacttagtg
    18121 ggttggcatg ttactcacta attttgaagc ccaatggtgt cagagaaccg cactggcatc
    18181 ctaatgcttc tgaattggat tatgtccttg gaggaagagc acgaatgata atattaagtc
    18241 ctggtggtag annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaagacata ggtatctcag gaggacttag
    18481 tgcaaattct aacgaggtat taggtgctac ttttgatttg cctcctacgt actttaataa
    18541 acttccaaga tttgaacggg atctttttgt agttgcagga ggaggataac ccgcgatcaa
    18601 aacaatatct ataatctata aatgatgggc accgacttaa ctgacctact aatggatgta
    18661 gtcgctggaa cagaatagat agttattgtt atagttattg cattactcat ttttggagca
    18721 aagaaagtcc ccgagcttgc cagatctttt ggtaaggcaa cttcagaata cgataatgct
    18781 caatatgagg cgaaaaatga catgcaacgg attagaaacc caggaacaac caatatacta
    18841 gagagcagag aaaaactgga agcagtagcc gatacattgg gaacaaaata ctcagattag
    18901 aatagccagg aacattgggc tgccattcaa agagaagttg aaaaggataa aaaaagagcc
    18961 tactagttaa taataacaac aacgtttgtt tactgagaga aatatttatc atatttgaag
    19021 aatgagaaaa aaagtatcat attctaagaa attctgattt agcaataaaa cattttctat
    19081 gaaatgatga aatatcactg ccttgcgaga aatttttgag cccaccaata agacaaaatg
    19141 aagaccaagc aaatcgcatc aagagatcca aaatgacgaa agctatgata aatacaaacc
    19201 agtaatttta cttcctgcag gtggtggaca gatgaccact caaagcaaag aaaacactac
    19261 tgatagatat ccctaaagcg actccatcct cttctttggc cacaagccac tatttatatt
    19321 tccaaaattc caacttgcat atattatgaa atgcgcctgt tgcggaggag acatgatacc
    19381 agataaaatg tcggaaaaga caatcgttta caaatgcaga caatgtggtt taagcgatac
    19441 tagattaaag gagattatct gaacccaaaa gggttgtgtt tagactcttg atccgccttt
    19501 agtaaaacaa taagtctaga cagagcgatg actatcttct ggagctacag acaataagga
    19561 tatttgcctg tccaaacgga aagtcaaata ttaataattt ttgcgatacg aaaaaagcag
    19621 cagtatctta accaatactt aattactacc gtggttaaaa tattcaaaga taaaacatgt
    19681 cacaatcgga agctcctctt aataatatat ctccatcggc aattatgcaa atggctactg
    19741 gcttttgggt atctaagact ttaatgactg cagtagaatt gggcgttttt acaaaaattt
    19801 cagcctatca aaaaaataac aatggtatta tgactttgag agaatttcaa aacataataa
    19861 gaattgaaga gagtagacca gcagaagcat ttacgacagc acttgtatct cttgggttat
    19921 taaaagtaaa taagaataat aacggagaaa aaacattcgc gaattctgaa gtatcttcaa
    19981 tgtttcttga taagagtaaa tctacgtaca taggagacgt catagctatg tttgacgaac
    20041 gtctatacaa aagatgggat aaactttcag aagctcttaa gacaaacaag cctattggag
    20101 aaggacaagg agaagatata gaaagtatat ttgacaaagg aaaatctaat caagaagtag
    20161 aacagctaca aaaatttact catgctatgc atggagttag tatcgggccg gcaatggcat
    20221 tggccaaaaa tgttgatttt tcaaaccaca aaaagatgat ggacattgga ggagggtcgg
    20281 gagtttatgc gatacaggta gtggcgaata atcctaacaa tatgtctgct gtagtgttgg
    20341 attctaaacc tgtgtgccag gttgcagatc gatatataca acagtacaac ttgcaggata
    20401 aagttcagac aatgacattt gactttttca aagatcaatt gccgaatgat tgcgatgtag
    20461 cgtttttgtc acatgtctta cacatatttg atagagacaa aaacattgct ttgttgaaaa
    20521 aaatatatga tagcttgcct aatgaaaatg gtacaataat aattagtgaa tggctactta
    20581 atgacgaaaa aactggtcct ataccttctg ctttaatggg attgacgatg atagtagaaa
    20641 atagtggagg aagaggttat tcttataatg aaatattgca gatgctcaca gaggtgggat
    20701 ttaagaatat agagagaaga cccttaattg aacctgctga gatagtcata ggatataaaa
    20761 aataaaatca aagataatgg gagtaagaga ccggaattcg ccattctctt acagaagagg
    20821 atatgaagta gtatttacag ggggtaataa gcgcaagcat cgaatagttt taaattctct
    20881 ccaagttcta actttatgcc cgctttttcc aaatacaatg acaaataatt cgaatgatgt
    20941 gcagatttgc agatacaata agtccaaatg ttacgtaatt gttatcaaaa ttatttattc
    21001 atctgaagat tgcggttgcc tcttctccag ctcttctaag acctgttcgg ggtcaacatg
    21061 aagctgttct gactctcgtt gttctttttg taattctttt gcaatcttta ttgctctagg
    21121 ctcttctttt ttaggatcca gttccccttc atcgtcagca ttagaattct catttttgtt
    21181 tgtcattgat tttcatcact tcggctacta tatcaagttt gaaaaaattc aacaaacacg
    21241 aagaaagaga gaaataaagt tcaccttgtg aaatcgtgta agatgactga cgcatccaca
    21301 cgtcttgatg ttactgaaat ggcaaaatga atggaatatt gtcacctgaa gtgtgtccat
    21361 gaatctttgg aatacggatt atagttagaa tggtttatag taatcttcgc tgttattatt
    21421 attgatcttt atctgactcg gcacttgggg aaatacgatt tggagaaaga acttgaaagt
    21481 gttcagctca gacaagaaga aataagactt ttgctagata ctctcagaaa nnctctatag
    21541 tacttctaga aatatacagt cccaatcccg tcccatcaaa tgactttgta gcaaatttag
    21601 taaataatct tgatatgatt tcacgatcta tcccataacc ggtatctttt acactaacta
    21661 ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnntacttgt aggtgcctga aaccaattaa aacaaaacat atccgttcta ctaccgccgc
    21781 tgtttgtatt atgataattt gggtagactg aacgaaaatg ttgctccctt tgttccatca
    21841 tcgttatact cagcccatat cgtacctcca tgagcttcta tagtcggtat cttttacact
    21901 aactattact tgcttgttta tatttttctc ttttacattt acaaatattt tgccttcctt
    21961 cgcaaatttt agagcgttgc tcaatagatt ggagatcact tcggtcattc tatctttatc
    22021 ggccacgata aaaatatccc ttggttcata tataacctct aatctatcat tatgagaatt
    22081 ttcaatttct ttcaatgcat cttgtattat atcttttaga ttaacgcttt ctttgtaaag
    22141 ctgtatggta ttgctttcta tcctagccgc atctaatatt acattagcca aatcttgaag
    22201 tttttttgca tttctacata ctattaaaac cgtttcttca tttggcatta aatgtttcaa
    22261 ttcgctgaag ctaaaaggag cggttgtatg ggagtcctga gctcatgtgc agcaatattt
    22321 atgaaatctc gctgcacctt gtcatgctcg tttaactgca cattagccaa cgaaagctgt
    22381 gaattggatg tttccaattc tcttgtcctt tgatttagca tttgcctcat gctatcaaat
    22441 tgtgtcgata attcccatat ttcgtcaggc tgggcatcgc ttttcatctc aaaatcaaga
    22501 ttaccagaag ctatttgcga tgccgcatct tttaaccttg atagtggttt aataattgaa
    22561 tattgagata tatgtctccc aatgacaaga gataccataa atgcaccaat actgctaaaa
    22621 atgagttcta tttgttgagt gcgaatagca tcgtttgctt tacctatttc gttctgaagt
    22681 ccagcttttt ctacgttcct caagttattg cttacggcat ctatttgacc gtgtatatca
    22741 tcagtcgatg tgatcatatt tagaatagat tgttcgtcaa tgccattcgc tttggaattg
    22801 attaactgca gagaaaccgt agagtatgcc aatgtcaggt acttaagtag tctataagat
    22861 ggcatttcat taggttgagc tatcttgcca aattgatcta tatacgtaat caatgtcttg
    22921 ttgtcttgta tcattaagtc tttcttgatg caacgcctta gcctccgaac ttcctaaagc
    22981 gaaacctaca atatcggaag atattttatt aattgatgac tgtatattat ctaaaactag
    23041 aagttttggt aaagtatcat tagtcacaac attgaaagca gagtttatgt gaaccaaatt
    23101 tacaaatcca actgttccca caataactac gaatgaggca atcgaaaaga atccaataac
    23161 aagttttgac ttaatagtac taaattggcc catatttttg attgtatcaa ctttataata
    23221 tgctttgtac ctatttctca tattgaattt ccaatatacc ttaagtttgt cgtttgcaag
    23281 ccattttcca aataaaacca atctttgtct tctttaactc tatcctcgat ttctcttaat
    23341 gttgccgatt ggcgtagatg tcaactattt tcaaaacagt aaaaataggt gtgatcacct
    23401 cagactctta tgaaagatgt actaattttt ctaggctcag gagcttcggt tcaattcggt
    23461 ctccctacca tgagacagtt cgtttgaatt tgaaaagcat ttgcaacaag gtcagaacac
    23521 gatagctcgc gtcatgacat gatacatatg ctctctttat gaagatatca aaagtacgtt
    23581 aattcgagtc cacggttaca cggacctaga atctgtattt agtgtagaga atttcctgga
    23641 gcgataaaac tgtggctgta tatgacatgg ccataactac ttgatagatc gaatgcctat
    23701 gcctaattgc gtcatcgcat aatacaattg ttcatcaaaa atcacatata gaatagctat
    23761 aatctccgtt gtgattaagt tatactaagt ccgtataaag gatatctcta ctaggacgag
    23821 tacatgtata caactacaag aagaacatcg actatccttg ccatcgtggt agtagcggca
    23881 acaacactaa ccttactgtc gtcgactact ttcacatctt ctgcattcgc tgcaaaaaca
    23941 ggttcctcgg gcaccaaaaa cttgaaaagt ctgtttgctt gtcagtctag cgcagctaac
    24001 gggcactttg ggcagcttac tgaaaccgca gtaatggatt gctattcgca agccttctca
    24061 agcagtcatg gagtttctgg aaataacaat aatccagcaa gcgatacagg cagtggtagt
    24121 agtactaata gtaccaattc tggctctacc tcgccaaccc atactgtttc ctctagcagc
    24181 cacacgcatc atcaccatac tccaacgagt cccaacagct caacaggtac cagtacaagc
    24241 tcaagtgccg gaccatgaag gcatgtgcgt tgaatagcac ggcatcacct cccttttttt
    24301 aaagtttaat taacgatgag atctcccata gctaattcta gataatgact cattcccttt
    24361 gtactgccac atcaagctta gaaaagagtg agattaacag ataatctagc ttttagaact
    24421 tggctgccag aactttatcg gtttgacctt ttattttcat aaccgcattc ttttgggtat
    24481 catctcaatc ggtaatttat catgattaaa aaaataatca tatggtctca gacattgttc
    24541 ttcattgata ttggagtttc tcactcatgt ttaacagcca aagggaaagt ggtgcatata
    24601 tgttctgagg tgattgtggt tatatgccag tcaatcttgt gtcaaaaggg tatagatggg
    24661 cattatttgt tatatcaaat gatcgactat ttgatgcaat ttagtatgga ttgcctcact
    24721 atgacaactg cataatgatc atgatactgt gttttagata ggcttgtctt tacaggccac
    24781 aatgtcagat atgtcagcgt agaatcttat ttagtattag aaatatggga ggatccattg
    24841 atcttatcct agcagccttg acaagttgct cgtttagttt gaaatgtatt cacaaagtcg
    24901 gcatctcctc ctcattcatg gaccaatttc tacgtgtcca tttgtgatga gaacatatcg
    24961 tcgctgcggt gtgtggcaac agttactata agcttcagga ttaagataca taattttcta
    25021 ccatgtaaag attcatgcat taagttatta actatgactc agtagttgat ataaaaacaa
    25081 aagctcattt gtagtttgaa tatcatttgg cttccagaag tatgtggaaa ggaagtgtaa
    25141 gttttgggtt agtcaatatc cctgtcaagg tatatctagc caccgaagat agagagtttt
    25201 cgttcaacca actatgtccg aatggtcata ggattcagta taaaaaatgg tgccccgttg
    25261 aagataaaga agtatcatat tctgaaataa aaaagggtta tgaggttaca aaagataact
    25321 atgtcgtagt tgaaaaagga gatttagaca agattaaact caaaacaaca aacacaattg
    25381 acattagaga attcattcaa gacaaggaac ttgacccaat cttaatagag aaaagttact
    25441 atgttgcacc tgataataaa agtgggaatg acaaggctta ttcactattt gtaaaagtgc
    25501 taagcgaagc caaaaagata gccataggaa aggtagtgtt caaggataaa gagcatattg
    25561 tcgctctccg accctatcaa agaggaatac taatgcatct gcttcattat atagacgaaa
    25621 taagaccagt cgatgaaatt agcgaattga aaggaataca gcgagctaat gtagatatta
    25681 aagaattatc attgggaaag ttactggttg aaaatctatc cagtgaacac tttgatgtaa
    25741 gtcagtattc agatgcttat gctaaagaat tagaaaagct aatagattcc aaagcaaagg
    25801 gtaaaacggt gctcgccaaa cccgcggaga gagtaaaaga ggataccaga gacctcgttg
    25861 cagcgctaaa ggctagcttg cagaagagca agattaaggg atcaaatgaa tgaattaaag
    25921 caaacgaact tctcttgtct tcaattgttg ctgtgattcc tgttcaggac ggtttccggt
    25981 tggaatctgg ttgtgagtag tcagaacaac attgttgcag gttcttctga taacccccta
    26041 cagcctcact gcaggcagca atggttggta agcaatagtt gcaacacatt gctaatnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26281 nnnncattgc taattttaca atagcacacc gaattttcca ggatataggg cattcagcga
    26341 tctctgcagc tgcaactaag gttttagata agttgttccc aaacgaacat aactattttg
    26401 acaaccgtct tgtcgaagca tctatgtcga ggttttgcct ggaatccact tcaaagaaga
    26461 tatcgacaat ggaatgagtg ttgaatctgt gataggtgaa aagatagtca acgatgtgca
    26521 gtgaatttta tatagccaag cacagcaggt tcaatggttt actaaatcct attagcggtt
    26581 cttgagggtt agacaattca tcaggtagaa tataaaggta gacgaagcaa agttattctt
    26641 actatcaagg cgaaagcaca tggaaaatat ttgtagaatg aatcatgtac agcttatgac
    26701 ttcgcttaaa gcaaagaacg ctttcagacg cttggattga agcctaaact acgatcaacc
    26761 atccatcatt tttttgcggt atgcgatacg acatattgct aactcaaaaa cactgtgcgc
    26821 aaattctcat tgagtgaatc gagaagtttc atgtcttcgg attcaagctg gaagtcgaag
    26881 acctcgctat tttgccggat tctctcctcg tgtatggact tcggaatgac gaccaagccg
    26941 tgttgtaagc tccaccgaat taggacctga gccgacgtct tgccatattc cttcgctacc
    27001 gctaaaagat ttggatggtc aagcctttct cccctggtca acgggctgta agcttccagc
    27061 tgaatcctat ttttctcgca gaactgtaac agatcctttt ggtataggaa tggatggaat
    27121 tctacttggt tcaccgcagg cgtaacgtca gagctcttga ggagctcttt cagattcggg
    27181 atctcgtagt tgctcacgcc aatcgcccgg gccttgctct cctcggacaa ccgaaccatt
    27241 gccctccagg tctcatcact cattccttgg actggccagt gaatgaggta gaggtctacg
    27301 taggtcaacc cgagccgtcg caagctcccc tcacacgctc gcaaagtcga gtcatatcct
    27361 tggtcactgt tccagacctt cgtggtgata aagacttcct ccctccggag tccaccttcc
    27421 ctgagtgcca tgcctacatc cgcctcattc ccgtatagac tggccgtgtc gatgtgtcgg
    27481 tagcctatat tcaacgcgta cttgactgca tgttgtgtta ttcttccagg aggtgactgg
    27541 taaactccta agccgaggcg tggaatctcg actccgttgt tgagtctggc ccgggatttt
    27601 aaggtcaata gcatgccccc tcaatgttaa agtgagtata aaacttcatc gcttggtact
    27661 aattcttgtt cggtttctcc atccataatg tgggaatact gaatcaccaa catgttcgaa
    27721 gtggtgaatt gttaatatgg caaattccag gcagtccaaa ttacgacctc ttccttactc
    27781 taattatttt ctttttatca ttattttgtt ggtctagtgt atgtatgtgt aagcgtagta
    27841 gatgtccgtg ctaatctgtg tgaagtagct catcaactta ttaccatgta agacgagctt
    27901 ttctcttgcc ttccgttctg catatttctt ctggaatcta aaagcctctc cggatatgtc
    27961 cgacgtataa aatagaatat atacgtcctt atttttgtta ataaattaaa gcagacatac
    28021 attgaagtat tacgtcggct gttcaggctg gagccaatat caaagatggg caaaggactt
    28081 ctatcctaat acactagttc ctgagggata tttggcatat tattctagaa ttttcgattt
    28141 tgttgaagtt tatttgaata gcattgttag tcgattaact ttcaaaaagt gggcaaagca
    28201 aactccggat aattttcgtt ttacactaag aatacctcaa gccataattc aaagtactga
    28261 tacagaaagg cttggacatt tcctagaaca agacgttgac cctttggaag aaaaggttct
    28321 tgctctggtg attcagccat ccactacgat taatttaaaa gatggtagag aatggctcga
    28381 cgaagtgctg cggatttgtg cctactatgg ctatcaagtt gtcatggaat ttaatcacta
    28441 ttcttggttc caggacttaa catatcatat tttagaaaag tataatgctg ctttggcgtg
    28501 gactgagaag agtcgaccag tcgtaacttc agattttttg tatctcagaa ttaatgatta
    28561 cgaagacagc gtaataaaga aatggattca aaaagtaaac gaagaacaag aggagacaaa
    28621 aaaaggaaaa gaacttgaat atacacttat cgttgttgat agacctgcaa ctgtggattc
    28681 ggtgttaaag ctgttgaatc tatctgaaag aaagaacgat gatcagaatt attggattgg
    28741 tagagtgata acgtgtgttg atttgaatgc cttctatccg tcctgtgaag aactccgtga
    28801 cccatcgcta ataggaaaac cacatgctgc aattatgact gaccaacaag aaagaaataa
    28861 tataacaaaa ggtgtagttg catcatcctc gtatgaggct aggaagcttg gtgtaaagtc
    28921 cgctatgtcc ctttcaaaag ctagggaact atgtcctaat cttatattaa aaccagttga
    28981 tattccatat tatcgccaag tttctgataa agttatgagt atgctagaag gatatgcaga
    29041 tgttctagaa caaacaagta tcgacgaagc atatttggat tgtaccaaaa aagtagtatc
    29101 aaaatataat cagtatcatt attcgaacat agaacactat gcgttagata ttaagaagac
    29161 aattgaggag caatgtaatc ttagatcatc tataggagta gcaccaacta agtcagctgc
    29221 aaagatggca tctgatttcc aaaaacctga cgggttaact attttctacc ctaatcaact
    29281 acaaaagttt cttgaaaacc tagaagttga aagagtatcc ggcataggcg ttaaaactca
    29341 gcaagttttg aaggaagaaa tgggaattca cacgattggt caacttgcaa aatatgatgt
    29401 tcaaaattta atggataggt ttggcagaaa gaatggactt tggatgtggc aagtagcaaa
    29461 tggtcaagat gaggacccag ttatacctag ggaagatcat atttccctta gtactgaacg
    29521 tacccttgaa tcattcacaa aggataaaca agtaatacta caattcttgt taaatgaatt
    29581 agttgacgag ctctacgaaa gagtatctag acgtgaatat cggtttaaga cagtagctgt
    29641 taagatcgtt aggtctgatt tttcagttga aacaagagaa acatcatatt caaattatca
    29701 gacaaggaaa gaaagtatat cctctgttat tgaaggatta ttggacaggt tctcgtttga
    29761 tgataacaca gcaaagataa gaaaagtagg actgaaagtt tcaaagttgg ttcgtttaga
    29821 aaacaaaaaa ccatcagcgt taaaacaaaa aacattgcta gactatagtt gaatagtgta
    29881 tgcttcaacg atatgatata agattcatca cgtagacata ctatttttcc cgtctttgtt
    29941 tgtttttcta gtgtcgacga ggaggcctat aagcagctat gatttaaatt cttgaaggat
    30001 gaactccata tctcaggaat tctgagctgc tcttaattga aaatggttcg atacaatacg
    30061 aataagggca tgcgtttatt acgtatacaa aacaaaatcg acgaaatagc tccgattagt
    30121 tagtgtgtat tgtgcttgct tattttgatt ggcagattct aaaagaatag aagatcacat
    30181 ttcgaaaatt gttaacatat ttttcagtta agagattcaa aaatcgacag tatcgttggt
    30241 aatggatcta ctgatgtctt ccttacagaa aaatccattt cgttactaag cggggtgttt
    30301 tctggattta tcttaattaa cgtagcttta atatttttag cgtgtaaagg caaagtgttg
    30361 gccggagaca ctgccaagga agcgcaggct attatcatgg catcacaagt catagaatga
    30421 attattgctt gcttccatac ctctcgtttt ataccctcac caaaccatac aacgtcatgc
    30481 cgtaagttac tttcacatgt cttgcaacta gggggaaatt tatctcatat cttacctttg
    30541 aaatcacatt tcgtacattt ggttcgaaga gtttccatga agctccgtga cctatctgat
    30601 tggtacaaaa aaggggtgac ccaggagcat gagaatcagc aacaaattca attaaggctt
    30661 ggtacaattt tcaaagagaa agaagtggat attcctcaag cttttggatc ttttgtcaat
    30721 gatgagggat gctctttcgg tccgttcgaa tatacaagga gccgcttact ctaattaaaa
    30781 tattatttcc atgtcaacgt gacgtgtctt agtacagaat actatacgca aaagacggta
    30841 ccgctattct gctctgtgtc ttactactac gtaacgctaa cgttattagc tgctgttcat
    30901 tattgatcaa actctcttct ttatcagaag aattggtact agtgtcggtg atccaatctt
    30961 aacgtttttt gttatctagt ttgatgcagt tgaaggtttt aagactgagg atgttagcaa
    31021 aggttagcgt gaataaccag gaaagcagca ctgagcgaca cattagatac cgaggtacgg
    31081 ctgatataat cgcggccatt ttgagagttg ttactgaaag agctgcaacc aatacacgta
    31141 tcatcatgta ccgaactttt ttgtcttcct cacaactaag acaatacatc ccaatactgt
    31201 tgggtaatgg tcttctggcc accgatgaag aaatcaatct ataacataac agaaaacggt
    31261 atgcgtctat tgcaactata ttaccagctc actgagatga tgaacaaaag gaaatagtga
    31321 ttgcgaatga acgaatgaac gattgaacga aatagtcgtc gatatcttta atgctaggta
    31381 gtgcgatgta gaatactggt atgaagggta caattgccgt tgataaaaca gagaaagtag
    31441 gaattactct tcctaaatca atattacaaa gaatcgataa agtccgtgga gatataccac
    31501 gaagcacgta cattcgaaga gctgttgagg tttatcttaa gcaaggaaag gggcggtgaa
    31561 tgactgctcc ttctgccagc acagtgagag gggggtactg gatcttcgaa aagatcaagt
    31621 tagtacatga ttgaatgcac gcttatgtca cttgcagtta gcttccgccg aaggcaatgt
    31681 taactatgag gaaaaaacta aaacgagaaa gattaatccc cacattaaca tcatgtacca
    31741 tcaatttttg atagcatttc caaaccttga gtcaccaatc caaaatcgaa cactccagaa
    31801 gacgtctaag ggtgatacta aatagctgga gttcgaacca gaatgtaagg caataccatg
    31861 gagaattcca tgagttttgg ttcctctgtt ttatcttgcc catcgaactc actaccatct
    31921 aatatcttac aacagctggt aaactcggtt cctcaataga aactataata taatatgttg
    31981 tcagaaatta taatttgatt atatttattg cgttaagatt tcagtcctag ttagctgccc
    32041 agctagattt ttccctggac gtgatttgta gccaggaact ccaataccaa ctgcgatcat
    32101 agtgctctta tctgcagcgt gaggagagaa aaactcagag acagcatcat caaaaaaagt
    32161 cgaaccagat gcccctattt tttgagcata agcagtaagg taaatctttc cagcaataac
    32221 tcctgcttca aattgtgatg ccctgtatcc tctattacca agagtatcca ggatcttatt
    32281 caagtcagtc ataagaaaga atacaacatt agcatcactg aaaagagatt ggcctaagca
    32341 aagataccca gatatctctc tgggtacttt gtttttgagt tgttcaagag attcattata
    32401 atgatcaaaa aaatacgccc cagcttgcaa cccttccaca tcgtttacta taaggtagat
    32461 atcgatggca ctgtttttgt tgttatcatt atcactttta ctatcaagaa aatccagcgg
    32521 cacgcctcgc gtcgaataat ggagtatatt agatctgtct cttatacaca tct
//
LOCUS       DAOR01000109            3628 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_34534, whole genome shotgun sequence.
ACCESSION   DAOR01000109 DAOR01000000
VERSION     DAOR01000109.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_34534"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1093 a          712 c          713 g         1110 t
ORIGIN      
        1 atgttattat gggtcttaga gaagattctc ttaaaaataa tggagatggg agagtagctt
       61 ccgaaaataa aataaaaacg aaagtttcaa ggcccgctgc tactttgtta gctcagaaga
      121 aaaagaataa taaaaataaa aatcttaaac aaatttttgt cctgtctttt gatgctttaa
      181 gagaaagaaa ggcaagatct gcactaacga tattgatggt tattgtagga ggtggcctga
      241 tgattgcaat taacggtatg agtgcaggtc agagtgcttt tgtaaacaag cagttaaaca
      301 ctttggcgcc taacatcatg tttgttagct caggtcagca tattcgcgga ggaccagatg
      361 gtccaccgac tattatcttt aatgccgagg ttgtaaatag gataaaatca cttccgtatg
      421 ttcaacaggt tattccagta tattcaggat cgcttcagct taatgcgatg ggtaatatcg
      481 aaaatgctca ggttatggga atggatccca caaaaatcaa cctaatttct ccttctatgc
      541 agctagtacc tggttcttca gtacaaccaa acaatcccac ggggatgtta gtaggcgata
      601 ccgtagccaa ccctccagga ttgacaactc catatgtaac agtgggtcag accgtaaggg
      661 caacttattc atttgcagat cctcacacag gaaagctaca aacagcatcg agaagtttta
      721 tagtaactgg aataatgcaa cctacaggta acaaccaaat cgacaaagca gtagtaatca
      781 atgaagctac tgccaattca ttatttcaca aaggggggaa atatgactca atggctgtag
      841 ctgcagtttc tgctgattac gttaatgcag tacaacaaga aattactaat atatacggta
      901 gtaataatat tggagtaata actcccaaag ccatcctagc agcaagacag cagtttcaga
      961 gtggaaatag ctcatttatc ttagatatcg catttatttc gttacttgta ggtgctgttg
     1021 gaattattac taccctttac acgtccgtta atgaaagaat aaaggaaatt ggaacaatga
     1081 aggctatcgg tgcaaagaat agctttattc ttggcctatt tttgagtgag gcattactga
     1141 ttggccttat aggatcaact ctagcaatat tggtgggtgg tggcggtgca tatataatga
     1201 cggattttgc tccgcggggc ccaggtggag gaggagcagc tgcggcacat gtttctccta
     1261 tctttattcc acatgatatt ctcaatgtct ggattctttc agtagtttta agcctagcag
     1321 caggactatt tccagcttgg aaagcatcac gtctttctcc attagaagct ctaagaagat
     1381 aggcctcgtc ggcctctgat taatgctgct tagctgaagt ggatataaca aactacgaag
     1441 taaaaagatt cgagaaggtt caaaaattag ggaatgtgta tagagtaaca aacaataatg
     1501 atatctctaa agttcgtgag attattgagg acatttattt gccgaccttt cagaatatgc
     1561 tggcgtcagt caaagttcct ccgttgtcgt cgtcagcagt tacactaact cccacatagt
     1621 tgtgacgtat cttgctgatg ggcatacgaa gtgtattttg atacggtcaa agacagacaa
     1681 aatattgtta cggctcaccg tggctacggc catttctcac cgctagacgc attccatacc
     1741 gcatcgacaa ctactcgtgc gatttctttt ttggtttcca aaacatgcat agaaattcgt
     1801 agaactgacg atactgagtg aggctcaaac tctaattatg aagaatctag accgcattga
     1861 ggccgacatc ttcttgatat tattgctcga agcaaagatt cttgatattg gtctagtcag
     1921 acaacaacgc cacctgaaat tcatgtttca ttctcatcat tattgggatc ttgtcgatat
     1981 gtcggaaaaa gtaaatcagc taaaagtaaa tggtatgttg tattatgatg ttttagaatt
     2041 gccactctgt taatcttatc tcaaattaat tcatctcttt taactcttgt attttggcag
     2101 caactagatc aaatccttgt tgccagaatt catcctttga gatatcaatc cccgattcct
     2161 taagcagatg ttcaggcttt tgtgaacctc cagccgacaa tattttaaga tattttggaa
     2221 taaatgactt tttcccttca gaataaaact gctggtacaa cgacaccacc aataaatttc
     2281 caaatgaata cgcataacaa tagaatgggg tatgataaaa atgtgggatg taaagccact
     2341 cccattgaaa atcgcgagag acatttactg aatttccaaa ctgttctttc aggttattca
     2401 gatataactc tgaaaccgta tctatcgttg catcctgttc agccacggct ctatgtgcat
     2461 caatttcaaa gagagtaaag tatgcttgtc gcattatagt tgcatacata tcatctattt
     2521 gttctgcaag taatatcctt cgttctacac gtgtaattct ttcagacaac ttgtcattaa
     2581 ggagcatttc cgcaaagact gatgcggtct cggctagtgg aagaggagca tcccacaccg
     2641 ttatcggttt atcagaagcg gtcatactat gtattgcatg tccaaattca tgcgccatag
     2701 tagagacgtc tctagattta ccatcaaaat tcaatagcac gtatggagtc atatttggag
     2761 atatagaaga acagaaagca ccgcctaact tactctttct aatttcagaa tctacatgtt
     2821 gctcattaaa aactttctca gcaaattctc tgaactttgg atggaaatct tcaaacgttc
     2881 tcagtacagt atcaatcgct ttaccatagg taaacctttt ctgatcagat gcctttaatg
     2941 ataaaggagc atatagatgg tacctctgca atttcttgac accaagcatc tttgcctttt
     3001 gtttaaaata gtcttggaaa actaccgagt ttcgcctaca tacagcgagc agagtctcaa
     3061 cggttgtatc gtctaagtca ttgtaagtat ttctgactga aattggtgat ctaaatccac
     3121 gcatggtcag gtattcgtcg cgccattcaa ttacacgatt ctgataaatt tcacccaata
     3181 cgccactatt tttcttgtat acttgcaaaa gggaattgta ggccgcttct ctctcttcag
     3241 actttgtact acgtatgagg gaaagcaatt tttccctatt agtgaatgtt cttttgattg
     3301 gcttcctacc ttttttaaat gtaacgacaa attcgaaacc actggtcatt ctatcataga
     3361 tcttgataag agcgctgctg cctgtaactt ccagggtggt gatgatcttc tcttcaggtt
     3421 ctgttaatgt atactttgca agtagccgtt gatgtttaag atactctcgg tagaccggag
     3481 atactgactc tataagacgt tgtgcagttt cttcaccaac ttcttttttg aaccataagt
     3541 caaagaagag aagcctattg gccatattag atccgaacat tgccattttt gttacaagtg
     3601 cagcccgttc attagaagag gtgtcagc
//
LOCUS       DAOR01000110            9243 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17504, whole genome shotgun sequence.
ACCESSION   DAOR01000110 DAOR01000000
VERSION     DAOR01000110.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17504"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2859..3087
                     /estimated_length=229
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4017..4236
                     /estimated_length=220
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7555..7807
                     /estimated_length=253
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2612 a         1656 c         1575 g         2698 t
ORIGIN      
        1 tatgcttcaa tatttgaaag tctttggaga caaactgaat tgtatcaaaa attaaaagaa
       61 gcagacaaaa tcaaagcact gggttgtctc ttagttctgg aaagtttatg ttatttgatt
      121 gacaatatag gcagagttct tctgcgaagg ctatctgtag atatctgtgt gcgttttcaa
      181 taaccttggt tagctcagca atctctatgt tttttaccgg gtgcatagga attcctaggc
      241 ttttgagtga agaggatgaa gaatagctat gcccatcgac acgaacgcca ccatagaact
      301 gcatacctgc tttgagacag caatcgcata caccaccaat aacacgtagt tgatttactc
      361 catgctcttt ttcttccaat gaataccatc tatgaggagc atgaactaca tgcattctat
      421 gattgagtgt atcaaatact ttctttgata ttccttttgg aattgtactt tctattgaaa
      481 ctaaagctcc actctttgct tcttttgaaa tcttttcaac taccgataat aacccatcta
      541 tttgaggaga aaacatatca tctggtttat gagtggagac acataagata aagacatcaa
      601 attcaccaaa atcaattgct tttccaatcg ccgctgtctt ctgcgccatc tccacagctt
      661 ttggacttat atcataccca taagtatcaa aacctctttc tttgacatat tttgctacag
      721 gtagtccaag ctgacccagg ccaactacta gaactttatt caattgtgga ggattattat
      781 catattgttc ttgatttgat gtctttattt tggcagctgt tgtctgtgca ccgtcggtaa
      841 cgctgggcca atttaatggc tcatgacaac atgctttagg actattatat gatattttga
      901 ataatcaccc tttggaattt gttatccgtc attcgatatt gaaagacact acagaactga
      961 atttatttaa atccagtagt aatttctaac taactggata gcacgaatct tactaactca
     1021 gtaagaaatg actactatat ttaattatta cattacaata caatcatgat gaaggtgacg
     1081 aataactatg gaatcagcat ttggtagaga aactagcaaa catatgaatg atagtaatga
     1141 taataacgac aataatgttg agtgccagga agtatctgat gcatggaaac tactaggtaa
     1201 gcgatggtct ttaatgatat taaagaattt gaacgccaag gaaagtatac ggtttaatga
     1261 attgaagaga gtgatatctg ggattagtag tacggtgcta gcagatagat tgttagaact
     1321 tgaacgtgag ggtcttgtaa ccaagaaaat ttaccccgaa atccctccaa gagtagaata
     1381 cagtctaaca tcccgtgcga aaggacttgg gattgttcta agctcattag ggaattgggt
     1441 tcgcagatgg aattccaaaa taaagattag taccggtgtc caaacaggaa ggtagcagca
     1501 atagacagag cagaacatat cagatgcagt tgacttggaa gtaaagcaag gctcgcaatt
     1561 taataaacga aaagtgacag agaatggctc ttagccagct gatctggcaa cagctgtgga
     1621 ttttgttttg gttgtaatcc cgggtacttt ccaccggcta gcccatgttc ctaattcttt
     1681 aaatattact cttagttctt ttgctcgaag tgttaaacga tattcaacct ttgatggaat
     1741 ttctgggtaa atcttagaac ttttttttta gaaacataga attcagaatt aaaatatgac
     1801 tgtattctac gtcgaatata ttggatgaaa tattggatgc gtgcgtcata tattgaatct
     1861 atatttcgaa tcccaactcc ttcgcattgt caaattattt ttgttcatcg cttgcgccta
     1921 gtttatcaag agctagattt ttggcatgcc ttgctttagc aagaatagga tcaatttcca
     1981 aggccttatt aaaacacgtt atagcttctt catatttttg caaattatga aagcatatcc
     2041 ccttgtttag ccacgcatca gcattagcag aattgatctt taatgattca ccgtaacagt
     2101 ctctagcatc ttcataattt cttaatcgaa gctgggaata ctgactctcc taagtgtata
     2161 ttcagtaaag atagccacaa tggtaattct cattcgccga accgagtcga tattgataag
     2221 ctgatcagag gagagataga ggtgatatct gaatttctgc cacttgtaga gcaccagata
     2281 ttaagaaaat tagcgccacg gtctgataga agcatttcag atatcattgt tttaatggat
     2341 atgccaggcc tacgtcttcc acctcaatta caatcaaggt tactagatta cattagatct
     2401 atagtaaggg aagaaaatga gaatattcag ttcattattg taacaaattc ctctgcgttg
     2461 atagataaag ctactactga ggaacttttc atgttgatgc catcgcagca gctggctgag
     2521 ggttccaacc agttggtgaa ggtttctgat tcggatatgt gttttgtacg tttctgataa
     2581 gtgttcatca aattgaaatg atatccgacc atatgatttg aaaatatcat ctgtcgagtc
     2641 atactagtac gaactatgac tacatagatt aggtgacatt cctcttatga tataacctat
     2701 ccacgctctg cgtgtgtcat ctgactcgca ttcgtctact catttatcta gcctttcgct
     2761 tttcttcctt ggaaagctta tgcattggca ttcctggttc tcttgatggc tcatctttcc
     2821 tagtttcgat agtatgtata tatgataatg cggtcatann nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnncct ttctcgatat attcatgtat gatgctggca
     3121 gaatctattg tgaataattc cttgtggtaa tcctcacccg aatccatatg atgatcccca
     3181 caaagatttg attcgacaag ccctatccac tcgactaaca catagccgac aggttctcct
     3241 tgccatgtaa ttcgcactgt caacttgcat aatacattat agaccgaatt tatgctttat
     3301 tcattcacaa aaattcttta ttctatgaca acgataacat aacaattgta gattgttgaa
     3361 attgtttctg tctactccgt gatgtcttgg tatgatatgg tcaactgtaa gcatgttgtt
     3421 cattaacggt atgttctggc atttcatgca tttgtaacca aatactcgta ccagtctctt
     3481 gaatctaaga cggttgttgg ctgacatttc ttatttattc tccctgctcc atatgttcat
     3541 tctcaaaaca gttgtaacaa tatactattg gtacgccaac gtcgtctaca tcataccaag
     3601 ccactggagc gccacatgaa tgacatcgta agggcttaac tttcattgct tatttttcat
     3661 ccttaaagta ataagctttt tgccatctca atccatcctt cttcatagca atatttctta
     3721 gcctaatttc gcatgtcatt tcttattcat tataatcgct aatctgagtt tagccgtagc
     3781 cgctcccagc cttggttttg ttcttagtcg gaaattacag cagggacaat ataacccctg
     3841 acagttaagg aagatagaac aaatctgaca tcttttctac cttgatagat atcgtgctcc
     3901 atcattgcca gtaggtctta tcgctttgta tcggtgacca ttacaaccac cgcttatggt
     3961 gattcacctc atagttcact tcaaacaatc ttcgataaag gccgtatcgt tgcctannnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttc caaattggag aatcacgttt
     4261 tgctcgctct aaatagattt ctttcttttt ccttattatc aattcaagaa taggggcaac
     4321 ctcaacacct tctttgcata gttatctgct gcattaaagt cagccacacg ctgctcagga
     4381 gtatattctt cctttcctcc tgcaacgttg tgtgtctttc gagttgcttt agctgcggtc
     4441 gtgtcaccaa cccaaaattt caacactaca gattttcttt cagcccgttc tttaacatca
     4501 gataaaaaga tattcgctat agttgagtcc tgttctaatg cctgtataaa atgccttttc
     4561 tcaatctcat gtaaatctga gagaaagcaa gggaatcgat cgccaaattt ttttctaatg
     4621 ttgttattca aaaattcatc tacatactga ataagtaatg aatgcttcaa aggagtttct
     4681 cttactgaga ctggtaattg cgtagtatta tcaccgcatt cgcgtgccca tctacacaaa
     4741 acaaacatag tagaatcacc aattccctgc aaaaatttag agcatgtcat acacacaagc
     4801 cacatagata tgcgaccatt atcttgaaag gcagctgggg ctaagtgctg tcctgttcgt
     4861 ggaatggtca catcatgacc tccaaattgg caaaagtcta gttgattaaa ggcattagtc
     4921 atttgtttag gattgtcttc tctctatgcc aaattgtcac ttagctcctt tataatttat
     4981 ttatatcatg ccgcattctt tcttctatat attctgatat aatagagaat aatttactaa
     5041 ttcaattttg tgattttttc ttagtttgcc gataaactaa ttcagaggct ttcctcagac
     5101 tacaggttat tcattgctgc acagggtaaa agaaataggc gaattggttc catccactag
     5161 atcgaattct agattaattg caagaactat ggcttttgta agaattcctg tattttttta
     5221 gacaaatttg cctgtcctct tcctgactac ttcgtaaata aatctaaaga actaatcgga
     5281 ttaatcatgg gtgtgctaac ataattaacc gataattata aaagtattct gttattaatt
     5341 taatgtaatg acaaaccgaa gtgcagaatg gccattattc ggcgttattc tgatcacctt
     5401 gatgctcaca acatcttcaa tcttcgctag ctcagtccta gcagttaaga atggtagtag
     5461 cagtagtgta acaactgtcc ataagacccc gagtaaaact accacagctc atcatgtcaa
     5521 aggtgtgaag gtttttcgaa ttcataccat accatcaaag gtagctattg gtaatacatt
     5581 cggtctacgg ggcattgtgt ttaacaactc cacagcgaca attacatttg ccaatggaac
     5641 atgcacttcg ccgatctcca tcacatttaa taagaatgtc atgatacaac ctcaggcaac
     5701 aactgcctca tgtaaagcgc aactggttac tctaaagcct ggagaacaat cgcctatatt
     5761 gagtccaaat ctctcaggta taacatacag agccacagct cctggaatga cgaatgcaac
     5821 aatgactttc aaatatggag ccgtaaccgc tacgagtaaa tcgcctatta gcgatagtgt
     5881 ttctagagta tactcattca atatacttcc tgctggttcc caacctacga gtttgcagca
     5941 cagtccacga tctactccta gttcgcagcc tggtgtgctg aacacagtac cataggctaa
     6001 gtccaaatgt tttagagtag tcattggtcc agagcatcct taccatctct atcatatatc
     6061 gtaaacatct ttggtctcaa actgaatatg agtgcaaaaa ccactgatct gtaaagtcgt
     6121 ggtcatgaaa tttactgtcg tcacttaaat attatgttgt gataatgaag ttatggccgc
     6181 agtattatca tcgacactat agacacgatt tttgaacccg gcagatcact aatttaagac
     6241 atggtctgat atggaacaag agtattcttt ccaggactaa ggaatgctct aaatactact
     6301 accactcaga ccgtcgttag gaaaatatta tattgaagta tagttgtcat gtttcgaaaa
     6361 gctcacaatc cacaatccat ctctgcaaaa tattctaagc taaaattaat ttgctcttat
     6421 ttcaatcgga ccgagtcctc tgttttcaag taaaatcata atgggaatgc tgttgtaggg
     6481 aaactttgta gagttataag ggccaagatt atcctcgatg tgtgtcactc tcgtcgcatc
     6541 attgtacaat gatttaatcc gttcgatatg aaatattgaa ttagttgaaa ccaagaggaa
     6601 tggatttctt aataagaacg tcttttaatt tgtctttctt gactgctgcg ttatgtgata
     6661 cccaagccct agggctaaag tcatgggtat gaaaagtatt ttcagagcct ttgaataagt
     6721 cgattactac agttttaagc gaacttggtg gacctaggat tatacctgca aatggtagag
     6781 tacaatccct gatattcagg attgttttga ctgatagtgc aaattctaac gctggccaga
     6841 gatgaggtaa attatttgat acaacctttc gcaggttttc atatctttca agtaatttct
     6901 cactccatct tgctatatca tcttctttat tcgtattagt gttccgattt tgtaggacct
     6961 ctttttgatg ttcgattggc tcttcattat catttaccac ttgtttagca atgaagtttg
     7021 ttgcacattt ccattgaaat tcttttttgt tgaaggtgga gaacaattta tcgttgggat
     7081 acagactgca aagaagattg gattaaaaga aacaattaga ataactgttg caggaatatc
     7141 ttttgcaact atactgtttt ttttattatt ctactcgcgt gcgttaattt caactagatg
     7201 gcttgaattg gcacttggaa taacactgta tttcttcgct gctagtatgt tcaaagaggt
     7261 acttgagaaa gaacatgaag aaaaagaatt tgatgaaaag gttcacaaat acggatatat
     7321 cacgattgta agtttggctt gggggtcaag aaatgtacta gccaaatcct tagatttgcc
     7381 tgatggaatt gtagaatgtt gtcaaagatt cgtaaatgcc aaaccactaa agatagatgt
     7441 tatttcagca ctaataacaa cagcggcagc agctccacca gctgctgcta acgattatcc
     7501 aaaagaagct acaacacgca tttttcttaa tgccgcggag ataggggttg gcgcnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnngtg tgaggtttct atagatgatg gacgccagag tttactttcg aagatgacta
     7861 tgggtgtagt ggccaatggc aggtatctag gaggagggtt tagagcagcg ccgcaggcaa
     7921 gcatcacgga tggtttgtta gatttaacta ttctcaagaa ttcaggaagc ctcaaaatgt
     7981 tagacgaatt tgtcaatatg aaaggcgatg cagataacac acaggagaag aataacattt
     8041 tctacatgca ggccaagaag gtctcaataa aatcaaagga gagagatatt acagttactc
     8101 ttgatggtga accgataggg attctgcctg caacattcca tgtttatcaa aaggctttga
     8161 gtataaaatt ctagagtatc ttaggtagat ctgataagcg attttattcc tacctacata
     8221 ataaccacgc tattatggat tttatgggaa gcaaaacata gaagggttga agacatagaa
     8281 aatatgtaca tgaatccgag cagtaccatt ctcttttgtt tttgtaggaa cagctatcaa
     8341 aactcaaaat tgtatcgact atcaaacctt ttaaaggcca taactctatg aaaaatatat
     8401 gtatacagct agtattgaat cgatgagaga ttttattaaa atgccgaaag cctccacaat
     8461 tgtgtttaga aaactcatag tagctatacc aatagtgcta cttcttcttg tatccatcgt
     8521 acaatctgtt cagctagtga acgcacagaa ctccacgatg tctggtggaa catcaggtaa
     8581 cacaactata acaacaacag gcaataagac agttataaca acatctcctg ccaccaacat
     8641 gacaactgga gccgcatctg cttccactac gtcacctagc gctagtggtc ttcccaatta
     8701 taatagaaca gggtttacta atctgtatgt tctcacaatc aacggaaaaa attttccaat
     8761 ccaatatagt atcacaggtg gcaaactggt tgggattctg gcagataaag atagagcttc
     8821 gtcggtacta gtccttaatc caagtggcaa tggcgggaga tttactattg aattacctag
     8881 aaacgctata gactcaaaag gggcttcaaa tgctgacaca aaatatctaa taaagataga
     8941 tggcaaggga gtagattaca aagaggtagc aaacaatgtg aatgcaagaa tattgtctat
     9001 tgactttagc aaggacaata gatttataga aataataggc actcaaatga catcgtgata
     9061 atgtaacctg tcatatcatc gtaggcgaaa gagataaccc agcgactcgc gcatgcacac
     9121 taataatcat tacaccagag gatttctgta caatacaacc taccgcctac aaagcgagag
     9181 gttacgttgt aaaggcctgc tactccgtca ttgtcaaaaa gggtttgtca gtctcttcac
     9241 tat
//
LOCUS       DAOR01000111            3857 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_86235, whole genome shotgun sequence.
ACCESSION   DAOR01000111 DAOR01000000
VERSION     DAOR01000111.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_86235"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    211..445
                     /estimated_length=235
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2381..2682
                     /estimated_length=302
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1174 a          576 c          615 g          955 t
ORIGIN      
        1 tactattgtc ataagctctt tgcaagcagc tatattatca tgagtaattt cagtaaggta
       61 tctaagcttt acacctctac cttttgcatc aagaaaagct ttctttattg gttcaagcat
      121 gacggctaat gatggtcgtg tgtgattcat gcacgtatca attcttttct ttgaattcgc
      181 aaaaaactct aattctgcat cgattacatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnttatt tcctttaact acttattaaa cattattttg
      481 atatttacta attacacaaa gaagagcaag ctgacgacga aaggccggaa acgcggcggc
      541 aggtgaacct cttaatacca ttccaggtgc aagactaatt cttgactatg aactcttttt
      601 ctgggatacg atttacaaac actcgattat ttacgcaagc ggcagatcat aaagttagtg
      661 ccattcagtc tgcctgttgt tcctgtcttt tgagtgttag aactggaatg ccaagcatac
      721 taacaccgaa ggaagggtgg aatcacaaag taagtcttag atggtttgct gtccaatatc
      781 aggagttatt taacaaaatc atcacatcaa aaaacgataa cgggacattt agtaaagaag
      841 atgctgagaa gcagaagata acgcttcccc atatcgaatt acaagaacag atatctcgga
      901 aactgaaaaa aatagataaa gtaataacac gactagagaa gctgaaacaa gatgcagtcg
      961 tattgacgca atcaaaacta atcggctaat ccgaagaatt tttacaggcc tagagactga
     1021 ttcgttcaag agtcactatc agaaattagg aacagaatat cttataattt tacatccaag
     1081 atttagatga atggcatggc aaggaattct aaaatttggt tattgataga tcccttatga
     1141 ataattcgat catccttttc ttgccagata ataattgcaa ggatgttata gacattatat
     1201 caaaaggagc ctatccgtat ctatccaagt ctgaagtaac atggataaat aatgacaaca
     1261 tgccccatac cgtgacaagt gataaagcag ggatatttga ttcatctatc atttctgcac
     1321 accaacaatg gaaagatacc tttaacacta ttggatcata tgcgtatcac tgtacattgc
     1381 atccttggat ggaaggaaaa gtggcggtag tagtagcaaa gtgatttctt ttactttttt
     1441 tgagtataca gataggccaa agtatttttt aaattgtttg agtaatctaa taaattgatt
     1501 atgagaacat atagaggaag gtgtgatatg agataacaac aacgattgat tacaactatc
     1561 ttgataaatt tagcaatgaa gtgctgaatt tgaacaaatc gattgggtgg ataggcctta
     1621 caaataaaat tggagttcaa ctaattgaaa aatatagaga aggattaagt gtattcctaa
     1681 ctaaggagga aaatgaagag tatgcgtctc atacaataac cagacacaaa aagagggcaa
     1741 aatttgaacc aaaaataggt gcactggatt atgcattggt caaatacgaa aaagtgatca
     1801 gagcaattat tccaataacc aatgattatt atttgcttat tgcattagat gtagaagaaa
     1861 aagattttga taggataata atggaaaaga ttattccttt tatagaagga gagaagcata
     1921 tctttgaggt aacagatggg ggctcgaata aatgaccatt caaatggttc tattcacaat
     1981 gacaatatgg ccttgctata ttaaagcacc atcaatcaag aaccggcgtc agctaatgct
     2041 ctccttcttg tcttatgaat atggagactg attaagcaat gatagattac gaattcctct
     2101 acgcttttag caaagatgta ctgaatctag acgagtctat catatggatt ggcattacga
     2161 acaagttcgg tgttctttta aatgtcgaac aacgacaaga acgggcactt tttttaactg
     2221 aagaagaaca ggaagagtat gtgtcaaatt caataacaag agacaaaaag ataagatttg
     2281 aatcaaaaat cggaaaacaa atctatgcat ttggtaagta taagaaactg aatagggcca
     2341 caattcccat aattaatgat ggttattaca tgcttctaat nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattgtagt tgacgatgct
     2701 gatggtgtgg gggtgaatcg agtcaattgc cgttcattcc ttcatcgtca tagtaaactg
     2761 tatgtaattg taggagataa ccaagatgag tacaatgcat tacactggat gctgtctctc
     2821 tagttgcaga aaaatacaca tactaaagca gaagaaatat tgtagatctc ataaaaaaat
     2881 actggaagta tacagattta aagtcttgct catcgattaa gatcaagcag aggcattgaa
     2941 ttctcttccc aatgatgatt cttcttttga tatcgcagta ctcttcgcaa tatacattag
     3001 cagaaatcct attccaccag ctactactcc agcaataaga accagaatgg atgcagaaat
     3061 tggctcaaca aggggaccta aaatctcatg cgcctggaca gcattaaatc cactaccacc
     3121 aacgattttt ggaagcgttg cagcacctcc tatatagcca gcatacatca acaagcccat
     3181 tgcagcagtg gttccaatgt tcataagcga aaggtggatc caagccataa ttttcggaat
     3241 ggtatgtctc gaatagtatc ttcccataac tttttcaaga tagtaataga agagagcaga
     3301 gactgccatt cctactacac ctacaactac gtacattaca tatccaagcg tgagccatgt
     3361 tccagcacca ccagctgcta tcactcttga aacttcaggt tttaggatag atatttggct
     3421 taagattaga aatactgtca atcctacaat aattgctcct tgtattatgg cagcaccaat
     3481 aaaccgtcta gtccatgtgc tttcatagtt gttagctcta ttgcttgaat tcatatattg
     3541 tctacggcag aacagtatac agaatagtct attatttagt aagagtacag tgtaatgctg
     3601 tgcatacata cacacataca tacaaacaaa gaaaaatatg tatgagttat aaagcgcatc
     3661 agacatagaa atatctgaaa tgtatcagcc gtatttgcag agtctgctga agatatgttg
     3721 atggccttcc acatatacca acatacaata aacataacaa taatatcttc aaaaatagag
     3781 gtgatgagtg taagcatatg ccaatagtca ccgtagcaat gtttagtgat agaatacaaa
     3841 cagaaaaaga tagacta
//
LOCUS       DAOR01000112            4057 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_68003, whole genome shotgun sequence.
ACCESSION   DAOR01000112 DAOR01000000
VERSION     DAOR01000112.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_68003"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    637..761
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1357 a          719 c          782 g         1074 t
ORIGIN      
        1 ctatcaacat gcaggaattt tcttttctta attatgatgt gtgccgtcgc gctcgccatc
       61 tctattcaag ttaattcgcc gtagtggtgt agatgccgat ccggtgttgt tgccaaagca
      121 aaaaaagaca ttggttccaa aaaatgttgt cattagggag gagggtgaac ctgaatgcaa
      181 aattattaat cggaatcttt tgaattccag atactttgta taaaaacatt gtgtagtagc
      241 cctgaagaag aagaatattg tagatacatt cttaattcct ttataattac tcattcaaat
      301 cttatgttga tatgtcaaac aaaccatcac aaagagacaa gcagattatt actgtagcag
      361 tagcattaac gtttggacta ggcgcattca tgtttccaac tatgacgaac tcttttgttc
      421 catcagcata tgcgttaaat tggcgtcctg ctcatctcaa tgtgaatttg catcaactat
      481 tagcacagat aaatcaatgc gtggaccaag gtacgcagtg cttcaactct ggaaacagcc
      541 aagcgaatat tcatttgcac caaggttggt tcaaccatat cgatttgggc cagtcattag
      601 cacaagtaaa tgattgttca ggcggtagta cgtgcannnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntagtacgtg cataaactcc
      781 ggaagtaaca atgcgaatat tcatgtcggc aaccatcata gtagtagcaa ttccgattac
      841 gcaaaaatag atcaatcaat agcacaagaa aatcattgtt caggcggtag tacgtgcata
      901 aactccggaa gcaacactgc caacatcaac atcaattaaa tgaattagga ttccacatag
      961 aataaacctc ttttttcttt ttaagataac ctgaaattag atcctaaaaa ctttatcatt
     1021 gttaaagtat caattcctgc ttggtagcaa attcgctgaa tttctcccgt ctagcatagc
     1081 taggatatcc aacttaaata tttaagatgg atttaggaga agattagatg tgatcaacat
     1141 aacacttcgt gagtcacatc taatgcttga ggattcacga taacgatatt tagtagtatt
     1201 aaaggtccac ttctagagtc atatggtcca agtcgtgatg tttccaagat ttgctcggac
     1261 atcctctgtt aaactgcgat atgttgtcaa agtattggat ggtagtgcgc catggcaatt
     1321 ccgtacgttc ttattggtat gataaaaata ttatatgatg aagagtaagc cacatttact
     1381 gaattgttgc ttctcatgag gtgaaaataa aataacgaaa acagaaaaaa tttttacatc
     1441 gctaagacct ttcgttgtcg atagtcagac tggcaaaaca aaattctgtg ccagctgtgg
     1501 taatttagct actcaggaag ccttattcag ttttggcggc ggagtaactg tcatagaaag
     1561 atattgtgac atatgcgcaa gaaaagtgaa gtaaagtaat tagccactcg tactctattt
     1621 gtgacgagca acaacaacaa tgagaatttt aacatgcacg cgctatagga catatattga
     1681 actcttaccc gtacaaaaac atgaggtcag ttcaaagaga taatagccta tatgattcga
     1741 agaatactat attgagttca tatgtttata acggataaat ttttcttatt atgtcgtcgt
     1801 gagataatga aattggagaa gattttcaga tcgatgcgat ccctaaagag tgctaatggc
     1861 aatgcgagac tttgtctagc gtgctgctac tcgggaagcg ttatttaatg tgggtgaaga
     1921 acttgctcta attgaaagtt attgtgacac atgtatgata aaaattaact aaccgctcct
     1981 gcaatccacc aagagtactc agagcttgat tctatactaa aataattata taatggatta
     2041 gaaattttat gatttaatga ttcaaaccaa agatactaat ccagccccga tgtcagttga
     2101 cgctaatttt gaaatgagga tgtcttacca tgctgtatag tcatcctgaa atgttaaggc
     2161 tggtatctgc tcccgtcaag cgtggcaaga atcgggatag aatacatata tttctaatgc
     2221 atacaacggt aagaatatga ttacatttat aaataaaaca caagaatggc cctcatggag
     2281 tacggttgta ttgacacatt cttagagacc atttctcgta tgaatgccgc tagcgccaaa
     2341 atatatggtc aaaggctcaa aaattttaat gatttctgtc tgaaacaata taatttcgat
     2401 actgacagaa taatcaacga gattaagctt aatcgcatag acccgtataa agtattgaat
     2461 caatttattg attacttgca atattcacat acattatcgc ccctgacact gaatcaacaa
     2521 atcataacga caaaaaattt gttcgagtat catgatgtta atatctcaaa cgcaaaatta
     2581 aaattcaaag tcaaatttcc caggactgtt cgcagacaca agcaagcctt gtctaaggac
     2641 gacgtttgta atatcattaa ttcatgctcc caaattagac taaagactta tgttatatta
     2701 ctagcagcca ccggaatgcg ggccgtagag gctttgtcag tcagaatcaa ggattttgac
     2761 tctaaatcgc gcaaaatcat actacggggc ggttttacaa agacgagaac agagagaatc
     2821 atttggctca cggaggaagc ctccaagcaa attgaggcat taatagaata caaatatcga
     2881 tcaagacgag tatgctatta taatgcacgt aaacatttgg ctgttaatga gtacaggaca
     2941 ccggccaaga acgaaaatga tttgattttt gccgttcgcc agaccaaacc caatccaaag
     3001 aacatataca atcacttatc cgcggatttt ggtaaaaccc ttgctaggat tgatatgaac
     3061 attaaagaag aatatgtgcc aaatcccaat agccggttga tacataaaga acgtagacga
     3121 attacgctcc attcttttag acgctatgtc aagtctacaa tcagtgattt gggatttgga
     3181 gactttagtg agtattttat tgggcataaa ggaagcactt actacaccaa gaccgacaaa
     3241 caaatagcag aaatgtttag caaggtcgag tcgcacttaa catttctaga ttttcctgct
     3301 ttagagcgta aaggcgccga cactcaagca aaagtggaaa tactagaaca agaaaatcaa
     3361 agactgagac aaacggagga atcaaacaag aatacgttag cgacactgag tgatcaagtc
     3421 gcagtactaa tgaatgttct tccattcatg aagtcgagta aaagcctcgg agccatacgg
     3481 aatgtgctta aagaagggat agaaaccaat aagtgaaccc atcagtcggc gattatgcat
     3541 tgccgaccct atattcaatc gtaggaagcg tgcattatcg gagcaagaaa ttcataatgt
     3601 cctccccacc tggatgaaac gccatgaatg gaaagcggca gatggatttc gaaagtacta
     3661 caagtaacac acaaccaata aacgtcgact ttgatggggc atgatattgc cccagagtca
     3721 tattacaagc cgaccgaacg cgatgtagtg caggattatc ttaaagcagc agatctatta
     3781 atagtcaatg gcgacacggc cgtaacaaca acaacaacaa agcaaaagca agtccagcag
     3841 ctaaaggaaa acgcgaagga cagtgagtac gttattaaag gaaaattaga agataaggag
     3901 aaggaaatac gatcgttgaa agaagatatg aattcaatgc gagatgatat gagtaatgta
     3961 ttcaaaatat tgaaaatagc caagcggaac aatggtagac tgggggagga cagaacgatg
     4021 ctagatgaaa aggggcgcat ctcgttctgt caagact
//
LOCUS       DAOR01000113            6644 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_68004, whole genome shotgun sequence.
ACCESSION   DAOR01000113 DAOR01000000
VERSION     DAOR01000113.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_68004"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3194..3483
                     /estimated_length=290
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1970 a         1229 c         1175 g         1980 t
ORIGIN      
        1 accttataca aacgtcaaaa gatagagaca atgccattgc catggtataa tgatactgag
       61 taaattgtat ggagataaat aaagcattag aaggattatg agaaccatgg attgaacacc
      121 agaacgaatg ggattaccca gaagcaaatt ttccttcgtc tattaggcag gtcaaagaat
      181 caccaatcga ataggtttta gtggccggca tctcaatatt attcagttag catgtttgat
      241 ttgcttccac gttggcctcg gtccctagtg ggatgatgta aaagaactat catactaaaa
      301 gtgaatatcg attggagcta actgctaaaa ttgatatctc tttataccag cgtgtcacat
      361 aataatagta taatgtcgtc atcctccaaa cacgatcgaa cttctaccac aaaccttact
      421 cccgaaaggg ctgcagaaac aatcaaaaat gtcgccagta gtattcgtga ggcgtcgtca
      481 acgataagag agatggtacg aacagtccac caaagcggtg caatcgaaga aattgcacag
      541 gcaatacaag aggcaagcat tgcaacacgt gatactgcaa gagaaataag tgatactgcc
      601 aaagacttga gagatcgtgg cattgttggg cgtacgactg cggctgtaga agaaacaagg
      661 gtggtaacgc gtcatacaac agacttacta aaggagacga cgcaggaaac agctcaggca
      721 gttcctcaga caacagagac agtaaaggaa ggcgctcgga ggataaagtc aatcaccaga
      781 aaaaagaagg ccaaagttat aagataaagc caatgcaatt taaaaaagca cgttagtctg
      841 tatcgttcgg tttaaaaaag tattagtagt cagtgaaagt acaggacacg tgaatttttt
      901 cacgctgcga agtgcattcc acgctaacac gtaatggcat ttatgacgtc gcagagggat
      961 ggagaagaac atgatcaaag ttgacttatt gcagggacaa agatgtaata tggacttgct
     1021 tctaactgaa ctcagcctaa aggtcttaga tcgctgaatg aaagcaagcc caatctatcc
     1081 cacagccagc taatttcgga gtatcctaaa ttagaaaaca agaactaata tatttatgat
     1141 caattggcta tagaaatatt ccgtaatgac gtctgctaat atgtacaacg aatttgttag
     1201 atattttgag aggatgggtg aactgtacaa ggaatatacc aaaggcatgg aaaggatcaa
     1261 tcaattatac actgaatcca ttaaaaatgt ggaaaagacg aatgaactgt acagggaact
     1321 tatcaaaacc aatgagagaa tgactgaact gtacaaggat atacaaagaa taaacctaga
     1381 ttggttagac ctcttttgga ggccttggat gtcaaaagaa caagaaaaga aggaaaatac
     1441 acagacagag gggaagacac aaaaccaggg ataagtaatt gacataggga ggtgtgacta
     1501 ttccaaaaat tttataattc atactcaggt cggcattaac tagattatat ctttatcagt
     1561 tagtgcgggt tggcattagc tatctttagt agatgtggtc ttccactgcg tgttctaacc
     1621 tgtaaaatag gattagacgc gtttcgcgat taaatgtaat aatgtttttt ctaatttgtg
     1681 actagccttt gttccatgac ccgtcataac ccgtatctat caggagacta aaatgggttc
     1741 gattaacaac tcagttggct ctgctgagat atagcagtct ggtggaggtt ttaaccgcaa
     1801 ttgatcccaa atgtaatgtt tttcctcctc tctaactgta cattctatct tcactcgtgt
     1861 actcccatct ttttctacat aacccttctc ttcccaacaa caggattcat ctagcagaat
     1921 atgattaaga tcattatgta acaaagacgt gttctcagct gacgatagta tttctattct
     1981 tacagggatt cgttttttta ttgcagcaat tactttaata ttagcttaca ggctatgtta
     2041 ctattctact ttgataagca gctactatga cttatcataa tccttactta ctagaataga
     2101 atagcaacta gtaggtattc agatatatgt ttgaaaacct agtttatccg agtatcggtt
     2161 tcgccactac tcttctagca ctagaaacaa cctggcactt tacagcctgc aaaatcactg
     2221 ataaaaagat aaagccctgc ttgttcaagc aagtaaagtc agctattgta cggccatagc
     2281 atcaggtgat ataatccgta caaagacgta gaaaaaagat agatgcaata caaagcaaga
     2341 acatgtctaa caagagaatt ttaatggtag atgacgacac ggatgttaac actacactaa
     2401 ggacggtttt ggagacaaac ggatttaaag ttgattcata tgaggatcct cttttggcat
     2461 tatctaactt taagatccgt ttctatgacc tggtaattct tgacatcaag atgcctgaaa
     2521 tgaatgggtt tagcttgtat agagaaatta aaagattgga taaaacagtc aaagtctgtt
     2581 ttcttacagc aggagaaatg tattatggag catatgacga tatattttct tcattagatg
     2641 ctaaatgtct tattcgaaag cctattgaaa acggaaagct aatagaacgt ataaatgaaa
     2701 taataaggcc aatttcgaca actgaaagat ttggcattga tgaagcttga agattttcac
     2761 atcatcttaa atttgccggt aagaatcttt cgtcgactcg ttctatcatg gtatggtatg
     2821 aatacaatct tgccaactgg tagaatagat ctatattatt tgcaacttcc tggagccatc
     2881 atagtcttag tctaatagag gaagaaaatg ttcagggtta aaggtcaatc atatatcctg
     2941 tggcaatgaa cccgaacccc ataacacctc attccatagc ataaacctct ttaacggcaa
     3001 gaccgtatcc aagactaatt ttcttttgca catatggcat atgtctagaa tggatcgaat
     3061 aagtattacc acactttgtt agaaggccct tcgctaccat ggctattaaa agtccggata
     3121 atttcagggg ttggatttca tatactgcac agaattcggc catttttact ctatattctt
     3181 ctacggtaaa gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnntttgctc ttgttgtatt attgctcttc tctttctcat gatattgccg ctcttcattt
     3541 tctacggcat taccatcatc ttttggtctc ctttctttgt cccgtccatg tctacgcaga
     3601 agatgcattc tgatactctg tctaaaaact tatgtcaaaa taaatttaac taagtctatg
     3661 ctatgagata tttttctaac ttctgttggt gctggtacga gcgttggata cgcgcaccca
     3721 ccacgataca tttctgtgtt ccaggttacg aaactgaaaa taaagaaccg ataaatacgc
     3781 aaactttctt caagttcaca gggatagtca actagtttag gtaatctctc ttctgatagt
     3841 tatcccttag actgactttg acttcaatgc ttcaatacga gattaacatt aaattgctat
     3901 tctgatatct ctcagcagat gtaagaatat gaagtgtttt aactgctatt ctgaatttct
     3961 accaacatta gtagagccct tatgtcaaga gtgtggcttc tgctactatt gcagagattt
     4021 cctatcatgt tttcactatg acatagataa agctcataac cataaacaga atctaccaca
     4081 ggcttctgta tcaccgataa agaaaaagag tattcaaaaa tgggattcgt acaatgatat
     4141 tgttcccaga ctaacaaact ctatccctga agacagcgca gcagtaatag tgatagatga
     4201 atcagtaaga cgcaaattag gaaaattgca tattttagac ctaatccaaa atctaacaag
     4261 tccagaaatg aattgttacg tagatataat ccccacagga acctctgaca atgatgtaat
     4321 taaggtcttt aaatcctatg taaatattcc tgcttttatg ttgacttcag ataaggatct
     4381 atatatgaaa ttacttggac gctccgtgtt tgtaaagaca aacagaggta atgcaatcag
     4441 aatagttact gaagcaataa aacacagaat ttcaagatca taattagacg attccatatc
     4501 gcagaggctt tgtgaatatc tcttaaagac caactggcat gtaatcatag cagaagcgta
     4561 tgattacaca cccacagtcg tattgaacca ttctctattt atcgagacac gcgaatataa
     4621 ttgtagcctg atttctctgg cctatcttta gttctccttt cgtaatcagt gatgggtcaa
     4681 agtatacaat gacactgtca tgaattcatt ttgctttctc ttctcttagc tgcttctcga
     4741 caatcggttt gcaactggtt ggtgcaataa actccaagta tcattaaaaa agctctttca
     4801 gtcttggggg agatgaatgc tcggtttact atcgggtata atgacttgga tagagtttcg
     4861 ttggcaatat gtttgggcag attaaggcca ggactgcccc agagcatatt tattgaaatt
     4921 taggccggtt atacatttaa tcccatatct cttgctttac ttacaagttt ttcagcgttt
     4981 ttatgtttcc ccagattgtt tagagatata gccttctcgt atattgtctg tgcatcatca
     5041 ggatttaatt ctagtgcttt atcataactc cttatggctt ccttataatt acccaaatta
     5101 gccagagata ttccaatatt ataccatgct ctgccaagat tgtctccaat ttgaatgacc
     5161 ttatcgtaag tcaccacagc ctccttgtag cgtcttagat tgtttagagc tactcctttg
     5221 ttaaactgag ctatagaatc gatgggattt atctctaaag tcttatcaaa gtagcttatg
     5281 gcttcttcgt acttttttaa ctggagaaga agcgctcctt tcaggttcat tgtatagaca
     5341 tccgttggac aaatttgtag agctttatca taatctctca gtccttcttc atatctccct
     5401 agtttgacta gcaccgcacc tttatcagtc catgctgtag catcattggg accaatctct
     5461 atagctttat cataactttc tatggcttgc tcgtattcgc ccaaattagc aagcgctgcc
     5521 cctctatcgg tccaagatcc gggattagtg ttgggatcga tctctatgga tttatcgaga
     5581 cattctaaag cctcattata tttttttata tcattgacaa gagccgcacc cttgtgatac
     5641 caagctctag ggtcattagg attaattgct atacccatat caaagctttt cacagcttct
     5701 tcatattctc ctaatgctac aagtgctgaa cccttgttgt accatgctat cgcaaggcca
     5761 gatcgaatct ctaaagccct atcaaagctt cttatggcct cccggtacct attcaagttg
     5821 ttaagagccg cgcccttttg ataccacgcg aaagcatcgt tggaatttac ttctaaagat
     5881 ttctctgctg attgtagatc atcttcatac ttttctaagg tgccgacagt tacacccctc
     5941 gtgcccctag tggggctaag tttgggatca atgtttgacg cctctttgta agacattaaa
     6001 acagcatagg tcttgccaat tatagtaata attgttacta tattgttact gaatatcact
     6061 atgtaactac ttactactgt gtgctagatt cagttcacca tatgacgacg ctatattaaa
     6121 gtcatatact accaatgaca ccgataaagg gaaatactta caaatgttta ttaaatatat
     6181 cagtttggag tcagtttttc ttacctgcct ttgccacacc atgacccagt tcgcaaatat
     6241 ctagtggttg attagctata tgtctgaaat gaagatagag tattgttctc ttgctttacg
     6301 tcaacgttca aggtattctc taggccatta aaaagttgtc taagctttga tcttctttaa
     6361 ccgctttcgg tctgattgaa tctgaacttg ctttaaggtt tggatgataa tacggaccta
     6421 gactgccttt cttattctta agttctgctt ccatcaattc tatgattctc tgatcataac
     6481 taatatgctg ttctttacaa taaagcttaa actcttctaa caaagctcgt ggagtttcta
     6541 ttgttattcg accgcttatc atatcagact agcattttat ttgtttatta gtttcagctt
     6601 taggttcttt tggttctgag ctgcagtgtt gcataacttg tgaa
//
LOCUS       DAOR01000114            4751 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_13900, whole genome shotgun sequence.
ACCESSION   DAOR01000114 DAOR01000000
VERSION     DAOR01000114.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_13900"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1454 a          935 c          938 g         1424 t
ORIGIN      
        1 tggtgtatat atagtgtata tatagtaaag ctagttaaaa tatccctcaa taacccttta
       61 ctgactgaca atataaaaaa aatggcaata accgagatac taacttcact agtacaattc
      121 ataacctcaa ttatttcaaa cctcggctat ggcggaatat tttttctcat gatactcgaa
      181 agtgcactga ttcccatacc aagtgaggtc ataatgccgt tttctggtta tctcgtttca
      241 gttggaaaat tgggatcagt aggagtggta ctagcaggtt catttggaaa cctagtaggt
      301 tccattgcta catactatct tggtttacgt gttggaagag ctgcaattat caaatacggt
      361 aaatatgtct tattcaaaaa gaaacatcta gagtacactg agcgattatt tgaaaaatat
      421 ggtgaaaaaa ttgcttttat tggtaggctt ttacctggag taaggacgta tatttcatta
      481 ccagcaggta tcggattaac tggcattgtc aagtttgctg tttatacttt tgctggttct
      541 ctgatttgga atgctttgct cacttacgct ggaatagaac taggaattaa ttggaagcac
      601 atcgataaat attctggata tctttatact actgcaatcg tttcaacaat cgcaataatc
      661 ctgtggatcc tttataatag aagacgagga aacaaatcca agacgactac aaaaatctaa
      721 gtagtagatt atctctgacc atcactttag gggcgatctg tttgttttcg gtcttatttt
      781 ctcatgattg acaattccaa tcatcatcgc cgccgtgctg ggaggtttgg tggaatagat
      841 tgtaacaata tcgctggttt gttgaaagag cacatacaaa cgggtttcgt acttttattg
      901 agcgtttgta tgcagacaga acgtagatac ggctatctat attaagaatc ctcatatagc
      961 aaatttataa cggatatttt atataatcta agcatgaaaa aatgttgtaa attaagcttt
     1021 cgaacactgc catcttatgt tggaataacg atggtgacta tgcttttatt ttctttgtgg
     1081 attgatatct catggattga cactacgtat gctgcagcat tcccgaacaa agccgaagta
     1141 aaaattactt cgccaagtaa aggccagcaa gttccaccag gcggaattct agtttcaggt
     1201 acttcttccg ctaatgcaac tactgactgt actgtttcag ttgtcataaa tggtataagg
     1261 ccataccaga gagcaatatc aacaggacat ggaggagcaa acgattactc aaattggact
     1321 tttacagcta ctcaaaaata tactgtaatc aaacaagggc aaaataaaat aactgccaaa
     1381 atgtcttgtc cccaaaacct caattttact aaattttata gtctaaacgt aacaggagtg
     1441 gcaggaaaag cacaacaaca actagccaat aatactacga ccacgggcac tgggtttccg
     1501 ccgtctcttg caagcgtagc aacgccgacc aatagtacta ttgcctcgtc atcaacacct
     1561 ggtggttctt cgagcactgg aagtagtgtt gggggaacaa tagtaaagga gccgaaacac
     1621 agtaagattc taaagagctc caagtaatgc ggataatggt agcaatcgct ctatatatcg
     1681 acaattccta caggcaggaa gggttgggaa gtataaagag tattggctga actggtgctg
     1741 tgagctatgg aaatggtttg taaaataaag catttgtatt tgacgtgata aatttaaggt
     1801 catgcttttg atttcgtgct agtttgaggt tggctagcac cctcaggcca gtgtctaacg
     1861 gtatgatttt attctggtga tgtccatcag attcacggta ttatcatgag cgaacaggaa
     1921 ctaccaccgt ggctaaaaga acagctgtca cgtctgcagc agcttcagca gaatttacag
     1981 gcgattatga tgcaaaaaca acaagtagaa atagaaattg ttgaaactga cagagcatta
     2041 gaagagctaa agaagatagg gacagaagat atagtataca aaagtgcagg ccctctccta
     2101 attaaatcaa aaaaagaaga tctcttaaag gagctcgggg agaaaaaaga actatcaaac
     2161 acgagagtga tggttctgac aaagcaagag acacgggtga aggacaacct taaggaggta
     2221 gaaaacaaaa tcaaccaaat gatacgtgga acacaaggta cagccggtgc cactgaatct
     2281 agttcaaaac cacgcagtca atagatataa aattttacaa gaaaatgaat gtaacaagta
     2341 caagcttaaa aagctaagta gaaagacaga aggttataac gatcgtgccg gttaggatcg
     2401 tagttgacga aagagaaaga cgcagtagga ttcccggtct cctgcgccaa gcgggcgcag
     2461 taacagattt tgcccatctt aaagtgggtg attatgtcat atctgcggaa actgcaatag
     2521 aaagaaaaac gatacacgac ctgttaaatt cagtatatga tggaagatta cttgtacagt
     2581 gctcggaact tgttcagcac tatactaaac cggtgataat catagaagga aatattttaa
     2641 ctctttctga tggcgctggt gctgctgttg aaggcattat tgatgataat gaaaggattt
     2701 atttgataaa cgaaactcta gcaaaggtcg cattggattt tagaattcca ataataaata
     2761 ctccatatgc agaatttaca tcacaattac tagtaacgat ggtaaatagg gcgttagaag
     2821 aaggtgcgag taagggtccg cttttaaagc ggataaagaa gacgaatcca gtccaaatac
     2881 aacaactctc aatactttct tcgctacccg gtgtaggcga caagcttgca gtgaggatgc
     2941 ttgagagatt tttgacacct caccgcgctc taaccgcatc agcagcagaa cttgccagaa
     3001 ttcccggttt tggcactgca agagcgctaa aggtgaggaa aatcttggat tcgcatgatt
     3061 ataaaaacgt agaaccttct caaagatcgc tgttagatgc cgagtcataa taaaacaaga
     3121 ataattgccg tttggttatt acaagtaccc taaatctgac taccgtaatc gcgaaattcg
     3181 gtcagaccct caaaattcaa atctcggttc cgttaccgat ctttactatg aatccacagc
     3241 tcatttctgg tcgaaatgtt ccctttatca agaggcattt ttccgaatac atgtagtatt
     3301 tgcaatcttt acattggaac ccctctgcac ccttgttgat gacatatcct tcctcgtaat
     3361 caagctttct tcctgtcttc tttatgaaat cccatacaat atctcctggc agcatcccat
     3421 cacctttcgg ggagagaaac tttgaaatac cattatttgc gatttctcct tcgactatat
     3481 gccacctttt ttcgtcgccc aaattgaatt tacatacggc acatttccaa tccgtttcag
     3541 ggctgctact ctcctgttct ctataatctg cctcgctttt ggaaatcttt ctcttggagc
     3601 ttaatacacc gaacagagca gcatccttcg tagccatatc attctcatct tgtttccctg
     3661 ttgacttggc aattgctgac tcctctactt cgttggtatt aatagctaaa tcacctaatg
     3721 ctgacatagc aatatcgtat ttatcaaata agtgagttaa tgcaccagaa ttttactgta
     3781 catctagaca cgcagaggta agcagataaa tgtcaatatt gttattttac tgacgttaat
     3841 aaaaaactgt taggctaaaa tcatactgaa tcaatagaac gactttttga attaatagaa
     3901 atagttgtca accttccctt gtgctaacat acctagcatt gaccattttt ttgttataat
     3961 aaacttgtta tagcattgca tgatgcagtt gcaaagatcc tgcctataag agaatatgga
     4021 atatcccaat ttggattatg taggagatct tatttaccag tccactacta agccggtcgc
     4081 tgtaataatc gacatttgtc agtatgcact acccacaaca taattccaga tggcgactca
     4141 ttaggacaaa aaatttaaga ctgcccagaa tgtcttgctt ttgattttac tttactcgct
     4201 gcttccttta cggtttccgt tgtctggggt gccgcctctg ctgtttgccg tgcagtatcc
     4261 cttattatct cagctgtctc acgcgcagcc atggttgttt gttctacggc agcggcagta
     4321 tctttgataa taccacgctc tttaagatcc tttgcggcat cattaagttc ttttgcagta
     4381 tctcgagccg ctacggttgc ttcgtaaact gcttgggtaa tttcgtcaat agccccgctt
     4441 tcgcgtaaag ttcttactgt ctctctcatc ttcgctgatg cttcacggat actcttcgca
     4501 atgtgcttta tcgactctgc agtcttttca ggattaactc ctgatgtttt aagagtgtcg
     4561 ttttttgaat ctgctggtga agtggacata ggggctgtat gcattacatc attgtataaa
     4621 gttttacttt tacttggtag gatatccgca ccttctctga tacatatatg catatatgca
     4681 cactaccatc atttgcaacc agttttttta tgactttgct gatttgttat agtttgtagc
     4741 tcaactcaca g
//
LOCUS       DAOR01000115            3202 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_125792, whole genome shotgun sequence.
ACCESSION   DAOR01000115 DAOR01000000
VERSION     DAOR01000115.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_125792"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1974..2257
                     /estimated_length=284
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          839 a          612 c          532 g          935 t
ORIGIN      
        1 tatttcttac caccttcata atgactctta ccaaaaataa ttttggaata caacctctca
       61 catagattcc tgcaaaccat actaaatgta aggaaaattt tggactgcta aaaatatggg
      121 agctacttat agagtgggag ggaaggcggc aaaatatcaa accttttatt cacaaacaca
      181 accaacaacc aatcaaccgc ataaccaata ttactattca tgaaagacaa attaaatcaa
      241 aaaagcagtc agtctagtct aatctaagcg agtataatgt agcctccctg tttcaggtta
      301 gaaacagaca cttgcttgtc atcttttata cgtcctaact gactcagtgt ttgtaacaat
      361 cttatttgtt cactctaata cttatttaat agtcgacgtt aggttctttt tcagttgttt
      421 ccatacgtaa tacatgtcag ttgataaata tcagcttgtt caaccagggt aaggaaaggc
      481 tcatcgactg accatccccc catcaactaa caacgtgtga cctaagacaa aagaagcctt
      541 atcggacaat aaccaaacta ctgcatttgc aatctcttca ggatcgccaa tgcgtccaat
      601 aggatgcatt gacttaaggg tttctagaaa ctgcttgttg tctccagctg cgcgtttaag
      661 cgtatcagta tcaactgtac ctggagcaat agcatttatt cttattccag atttggcata
      721 ttctagtgca gctgatttgg tcagtccaag cacagcatgc ttgcttgcga cataaattga
      781 cttttgcgga aatcccatta cgccagctcc agaggacatg tttactattg ctccgcctcc
      841 gcttcttatc atctgcagaa tctcatattt catacacaac caaacacctt taaaatttac
      901 gttcatgatc tgatcaaaaa catttgattt ctgttctact aatggtgtcg tggtttctcc
      961 tatgccagca ttattaaata cataatcaag cctatcatac gctttgatgg tcttctctac
     1021 aagcgacctg atatcatctt cttttgtcac atcggtcttc acaaagatgc cgtcacttcc
     1081 agcttctttt attaggtgca cggtttcctc tccttctttg gcacggcgag cggctaccgc
     1141 aaccttaact ccttctttcg caagagcaat tccggttgcc cttcctatgc cggagctacc
     1201 acctgttatg attgcgacct tttgatttaa catttcttac atatagaata tctgggatcg
     1261 cactatttat cgttgacatg gaatctgatc tgaagccgct ttcatacaag ttacgacaag
     1321 ccatatggaa atccaaatgg aacaaaatag ctctgacacc catatcataa tgcgtaccaa
     1381 ttgtattgtg ttcttaagct cctacgacaa ctttcgtgat tattgtaaaa tatgtaaagc
     1441 gaattttgca gttagacatt ggttactaat tagccagcag attactacct ccttaagtag
     1501 tgaagtcttc agatgaataa tagatggtat actctccccc tttttgttta gttggtaaac
     1561 ttactgctag cagtacttga gctatttgtt tttgcttcct gaactagtta ctatcctgat
     1621 tgttgcatga tgagacgact tctttatgac ctttatttaa tctatttaac ataggaagca
     1681 ggatgaattt cattccactg ctgtgacaag tctgtgacaa gtctgccgct aacggttaac
     1741 tgttgacctt gagggggttg cttattagat ggatatgtac tcgtaacgcc attacaatat
     1801 ggtccgaagt ggctatagct actggcgatt gcgttatggc atatgacttc tactattaat
     1861 ctatcacacg ttgcagtagc ggtatgagtg ggtgtttgga gggccacatc tgaataccct
     1921 gcagcattag tagcttttaa tgcacagttt tgtggtttcg ttaaacccag aggnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt taaggtaaaa tgcaaatcgc
     2281 cgtctggttc gttctttgtt cctgcgctgc tgtaaactgt tcctgtcacg gttatacagg
     2341 gtttcagttc cttcagcctt gaaggatcgt atacgtgctt cgctaaaaat tcggcattag
     2401 tagcatcagt agcagaacat gttatactag tagctgtgtt atgtgcaaca gaatttgtta
     2461 tttgtgaagg tgctctctta gtagaattac tactgtgtaa agtagcatta cttggaacgg
     2521 catttattga tgaatgtgat ttcgttgtag gaggctgtgc acctgtactc cgatactgca
     2581 taagaaaatg tcccaaatgc cacgctagat aggagaacta atagaacaat accaatcacc
     2641 atagcttcgg ttcgcattcc taattccaag tcttttcaaa tatataaaga tgaacaaggt
     2701 atttgtctca caagtataag ttttatggag gcaagcatct acatccacat cagggaaaac
     2761 tattctaatc ccagcttcca aagatatatt gatgccaaag ctctaggatt ttgccatttg
     2821 aaatgcgaaa gagaccactt catttaggag ttgaaaatct aggtgtcaaa aaaaacttct
     2881 acttagtcct acgtgttcta actgcatggc aatttggaca taacgctata caatctgact
     2941 ccttgttatt tgatctatcg ttatctttgt ggtcataatc aactacattt aacagcccat
     3001 tacaatgaca gcacctgtga tgttgctaat ccaaaattta tcacgtctgt ctagatcatg
     3061 gtactgcaga gcgtaaaaca taccgaaatt catttgataa gaggatgtct actgctgctg
     3121 ggtgggtgca aaaccaaaat cgtttcaatc agtcctagtt gtagtttttc ttgatatggt
     3181 cgtttacatt aaattacatt aa
//
LOCUS       DAOR01000116            4600 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_67452, whole genome shotgun sequence.
ACCESSION   DAOR01000116 DAOR01000000
VERSION     DAOR01000116.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_67452"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    902..1184
                     /estimated_length=283
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3790..4073
                     /estimated_length=284
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1199 a          775 c          824 g         1235 t
ORIGIN      
        1 ctgcatttct ccatgaattt ctatgatctt attatttttc cggtcttaat gtctatataa
       61 aaggattgca taaaggtcta ttccaaaatt attgttggta agagccatta tggagctggt
      121 aagaaatagt gtaggaggta tgaggtttat tattaccaag acgagacatc ttttcccata
      181 gattggcacg gcaatttttt ccgagaaggt tttgaccagt agtactccat aaacagtcct
      241 agacatcggc tttcattgtc ttattttgtt gttgtaataa taattgatat tccgctatct
      301 tctttctgga cctcaatttt gctctacaat ttaagttgcg cggcttcatt cttagcctgt
      361 aactacagca cggacacaag agcccttcat agcttcggtt attggtagct tagtgggaat
      421 agtgggcttt attgctgtcg cagatttgat ggcacattct acacgattat tcaagaagag
      481 ataactgatt ttttataata tcgcccaaat aataatttaa ttggtgctat ttttcatgca
      541 agatatttta tgagctctgc atacatgtga ggggtgagtg cgactaaatt gaggtggatc
      601 acgtagatca agcgagcctc acttgagtat gtcgagacaa ttcttcattt gcagaggcat
      661 gcttatctca gcgaggcgga gatctataat gattatggcc ttcctaaagg cataaaggag
      721 attaaacaag aattttcaca ggaggtattt ctgaaggcca cggaagaaga cgggaataga
      781 attggttcag tacgagcttc ttcaatcgtt tcttattcct gtatcacatg tcttatgtat
      841 tgctctgtgt cttcgttttc tgataatgct tcgacaccgc tacttttctc cactattttg
      901 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncactat tttgcatttt
     1201 ctgccgctgc gcatgatatg tactgtgggg ataagtcagt ttttttgttt attggtagag
     1261 cccttttttg ttttctgctt cagtacctgt ggctttttct tgtagctcct cagctgacgg
     1321 gatatgtcca aattgttttt caaaggcgtt cacttgtgct gttagccatt gtgcaattga
     1381 tttcgcctga aacggatcta ttatgagtct acactctagt atgcgactaa cgctaggtgg
     1441 catgggtgtt tgaaccgatg acaacgctct tgcaacttca agttgttctg agtaaagaac
     1501 tacttcaaag cgcataccaa caggcatgcc gtatactcca cccacattga ttgttctaaa
     1561 gtttgggtgt tcctttacct ctatttctgg ggggatagct gcactcattt accgtagatc
     1621 acttgaatta atggcaatat gtgtctattt gtgtcttgtt tgtattatat tataacgagt
     1681 atgccagccg aatgatatgc tagagtagct cttatgaaaa gaaatgttaa accccatcgt
     1741 tcctgaaaca acactcgaat atattgataa tacgcctaat agactagtta acaaaacgac
     1801 cgatatagca ctcctagcat caatgctatc ttgaacaatt agcacaaact aaaccctaga
     1861 attattaggc ccacaatgag ccgtcattga gacttctccg cgccgtacta ctgctccctc
     1921 tgaataatgc ttgatgaatt tcttatagaa cataataggg aatcaagata tagacaaaga
     1981 aggacaatta aaaaaataca aataaaatgc agtggtttga acgactcact atcttctctt
     2041 agtcaatttt acagagcaag gaatcaagga tgtaaaggat aatctaaagc gagctgaggc
     2101 ttttaagact gaagttgaaa agatgggcgg gaaggtcctt aacttgtatt atacacttgg
     2161 aaagcattgt agcaatagtt gaagctccaa atgacgaggc aatagcgtca gttctgtttg
     2221 ctactggaag cctagacaat gcacgtacta aaacacttaa agcatttcca atgtctgaag
     2281 cagctaatat aattaaaaat tttccttaaa tcagacatct tcgatacggg taacgtgacg
     2341 gcccagctca atcatgccac ttcaatgagg gaaggcgtca ggtaggaccc ataaacgaaa
     2401 ctctagtagc gtgcgacctg gtacagctgc ggggatgatc catcttcggc ggccatagac
     2461 catccaaatc attcggaaac ttgactaagg cgcaagacct tgttcttttt ctaaaatcca
     2521 atcccgttaa acttgtattt tctttttctc tagtatggtg ttctctcgtt tgaatggtat
     2581 ggaggtccaa aatctcacga acctccaaac ttatgatcga tagtcccgca tattagcggc
     2641 tgatcagttg caagtacgag cataatcaag gctcctccag ggatattgaa gacatgagtg
     2701 tgttaggtat acagctgttc ctgtaacaat agcgcacctc atatgggatg tacttttact
     2761 tgcatttttt agtaaagtcg gactgaaatt cgttccataa cgttaattcg cgtaatttgt
     2821 aagcggttgt accatcaaaa aatgggatga agccgtcgat ggtgatgatt aggaggatgc
     2881 tgactcatcg tctattatat tttcctcata tatttccctt atcgtaccaa tcccgccgta
     2941 cgaaactgta gtgtatacat ataattcgga atatatgtat agttgccgtg ccggagaatt
     3001 tggttcattg ctattaagtg gattctgtga tgatccttta gcagcagtag cgtagatatc
     3061 aaaattcagc agttacgagg ttaactagta tatcaattaa taaaacatga catgataata
     3121 gccattaaaa tggtatcggc aaattattct cagaaaggta agcgccctga tgaatccaaa
     3181 tcagagacaa ctattataat gctcccttca gatgcgaatc caaaaggtaa tgtttttggt
     3241 ggtgtaatac taaaacatct agatcttatt gcaggtcttg tggccaaacg acacgcagga
     3301 catgctaaca tagtcactgc aagtattgat aggatgacat ttttaaaacc cgtgtacatt
     3361 ggtaatgcat tgatactttc tgcacggctt aattatgtaa aaagatcctc tatggaggtt
     3421 gaagttataa tagaggcaga agatcttgac atcggcgagc gagttctaac tggcactgca
     3481 tttgttactg tagttgcctt ggacgaacat cgaaaaccaa ttgaagttat tcccttaatc
     3541 ttgaacacag atgaagataa aaaaagattt catgaaggtg aatctagaat gatgaccaga
     3601 ctaaaaggag taggcagagc ggtcacctag catctcctcc tgctgtgtct gatagacacc
     3661 acctagaata tctaaattat tactattagg taacacaatc atattggtac tggaggtacc
     3721 catacccgca aaagagatcc tgatcctgag ggattcgaac aactcgataa agagatccca
     3781 tgggcactcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctggag
     4081 agagtctgta tatcggcttt atttcccatt ctggatttag gccgccaaat tctgatagta
     4141 ctacaatacc gatagattta cctgcaagtt ttagagttgt aagtgtatag agtattggca
     4201 tattctgggg actcgtaggt ataattctag gctcattttg ggacaaactt aaaccacaag
     4261 aaactagcaa aattgcgcga acgcacaagt aactatcaca aaaccaagaa aagtactaga
     4321 aaaagatcta ccattccaat aaaaaaactg ataaagctaa aagtacaaag attgtgggtt
     4381 ttatagctga catcacgtta tatcacctgg tagccatatg tctactagtt gtcctttagt
     4441 tgtttcaaaa tctatatccg accaaccacc tatctgcaga ttgagatgtg ccaatgagca
     4501 ggtggggagt agatgaattt cactagttag gagttctagt aattcttcga tgccaggatt
     4561 gtgaccaact accagcactc tcctctggcc attgggtagg
//
LOCUS       DAOR01000117            4436 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_89401, whole genome shotgun sequence.
ACCESSION   DAOR01000117 DAOR01000000
VERSION     DAOR01000117.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_89401"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2284..2567
                     /estimated_length=284
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1185 a          869 c          740 g         1358 t
ORIGIN      
        1 aatcaagggt ttttggttga ttaataatga tctgtacctt gctgcgaatg tatctgatat
       61 gtcatcatcc gtgatgtctt gtagaaattg tcctctaatc aaacctccat atacaactaa
      121 atctcgtgca agcatcttta atcgtctgtc taatgagtta ttgatgattt ctgctttatc
      181 attaaaatct tcctctccgt ttatagaaac tccataaggc atactccatc catagcactg
      241 acgtactgct gttcgcattt gatccattac ggttaagccc ttctcatgag atgcacaaat
      301 gtaaccaaag gttttgccag caaactcttc ccagaaatgg tcgagaaagt ttttcataat
      361 gccagacatg gacccatgat aatctggaga tgcgagtaca aacgaatcag cccacttaag
      421 gagatgtccc atcttttgaa tattgttgtg agaactatca tctgggttat agataggaag
      481 ctttgtttga cgtaaatcaa ccagatttgt ttcagcgcca tagttatttt ttgataaatc
      541 taacaccatt tttactgcta aagtactaaa cgaaccttct cgcgtactgc cgccgacacc
      601 cactatcctg atacgtctat tctttccata attgccgaaa gaattcagag actcgttcct
      661 acttattgtt tcgattcttt ggttctcgtt cgtgtcaaat ctctcagaat tagtttcact
      721 ggtgatgtcc acaggtatta tgggacaaat ctaattgata aactatacgg tttacaaagt
      781 gtacaaatca tttattggct attaaaactt atcagagttc cctccataag tcgatgcaaa
      841 tatgtccttc atttctttct ttaatcacaa agtaactaat taggtcacct ttggcagccc
      901 tgttagttgt tttttcgtgg ctgttacaag taatttttca aagaggtggt ttcagtagaa
      961 tatgcttctc aatgcttttc tgtacgtgga tagtcggaca actatgttat aaaggaaaat
     1021 agctgtcata agtcttgccg ttgctttttg gattctgcag tctacccctt gattctgtaa
     1081 tttcccagct atcctcaaac ctaaagcaat tcatggaatg tgcggtgtaa ttgcatgaat
     1141 agtattatca taggtttgat attctgagaa ttctcgcaaa atggtcatga gtaattatca
     1201 ggtaaagttt caaattcata gattaaatcc cagactgact gttgtctatt ttcaggcttg
     1261 aggtcaatca ttttcgagat acaccggtcc agttttctag aaattgtaga atgatatttt
     1321 gacggaggat caactgacgg tctcttcgtt tgatcgtcgt acgcaggagg tcttttacgg
     1381 gtcagcattt catagaatat aattcccaaa gaaaaaatat cagcacgtcc atcaattaaa
     1441 tcgtcaaagg caaaccattg ttctggtgaa caatatccag gtgttccacc tatttgtcca
     1501 cctgccgaag cagtcaatgc tagtgattcc ctactgatat ctttaccaag tccccaatct
     1561 gcaattttta tctcagatct atcaggattt gtatacaaaa tgttatccgg ctttaggtct
     1621 cgatgataga tattgacact atgagcttgc gccagagcat tacaaacagg caacatgatt
     1681 ctgttgatcg tccatcttct ctcgaaaaca tatctttcct cagttctaaa cctttcatcc
     1741 atatgctgtc caagagaccc gccgcttaga tattccatta tataataagg actaaaatta
     1801 ggaggctccc ctcctatatt ccactggaat atcttgacaa catttggatg atttagttga
     1861 gaaagtatct tgatttctct ttcaaaacgc tccttattag tcgtattgag gtttattagc
     1921 tcctttaatg catacttctt gttgtcatct ccttcgattt catagaccgt tccaaatgct
     1981 cctgaattaa gtggttttat aatagtgtat ttcaattgta gatatagata ttatagtgtg
     2041 ggtagtaatt tcttataaat tcatgctatt ggaaggttta cgcaaagtcg agtactaata
     2101 gttaagctaa ttctgtttgg taatagttca tgttattgat acgttaaccc aggtattgta
     2161 ctgtcaactc aactagagca agaaatttgc tgttttgata agcagataag caggtattga
     2221 ccaatcaaaa taaaaccatg tcatatttgc gtataaaccc caacaaaacg gaagttcgaa
     2281 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag ttcgaaattc
     2581 actaaatctt gcggcccgat aactattgcc aaaggtgaat ttaattgatt taacacattt
     2641 gtattgttat tacagcttga attctgaatg taacgatcta gtaggagtct attcccaggt
     2701 atcttttttt gcctaactat acaatgataa tatagccaat cgttctttcg ttcgacctta
     2761 aatgaccact caattgttcc tcttgtgcgc aacagcatga atagacggcg actcgtatac
     2821 agttgataat ttcgcttttt ctattttctt cgttgccatc aatttagccc tatatttcaa
     2881 gtttcgtggc ctagtcctca atctataacc acagcacgga caccataatc cgtcccattt
     2941 caagaaaatc tcgcacatct gacaacgttt ctgccctata gaataacgac cagttgcaac
     3001 cggcttgatt gccttatgac gtacgcatat tcctttacag accaatttat tatttcattc
     3061 tccttttgta tttctactgc gtaatgatcc catgacacaa acaggtagca aacataatgg
     3121 cgtcgtgtat ctatgttatt acgttgctga gacatcggga gattagcgag atatacaaac
     3181 taaaactacg cagtttgacc ttattaggtt atctggtcat aaagtcagga ttcctattga
     3241 ccgacacaat ttctgtcagc cgaggcttta ttttaccaac cactgcaaac tagatccttg
     3301 catgatacga ggatttaact agctctcaag ttctgccttt acgtgcttta ctaaatcctc
     3361 tatctggata ggcttaacgt aacagtctaa atccattgtg gggaatattt ctcttagcga
     3421 ttcatagtaa acttcatttg ctgtaataaa acatgcttta acttcgctgt ctatcctttt
     3481 gattttctca tatagctgaa agccgttgat ctgtggcatc ttgacatcta ggagtaggag
     3541 atcgtatatg ccaggcttga agcttgatag tgccaaaacg ggatccttga aagcatctac
     3601 tacaaatcca tgtgcttcta aacctttctt aaaggtgaaa ataacgtccg gctcatcatc
     3661 tacaagaagt attctattac taccggagtt cctttgctca accttgatag cgtgttcccg
     3721 attggtttgc aaatagttat actaacaaga aaacgtggat gttctatatt attatgatga
     3781 tcgccacatg acggtagagt gttgcgccca tgccatgcca agatacttga ttgctttctt
     3841 gctggtaaaa gcagcttgac aagcactcaa gccattaagt gcgaccctta ctaatcgacg
     3901 cgcaactatc agtggaaaat gtcgacgtgg aaatgtctcg tgcctccatg cgaattccct
     3961 gagctaacaa ttacttgcca ttgcaatatg tccaggcgtt tcgtatcgcc aacactagtg
     4021 ccattgctat agccgtgcac actaatctcc cgtcaatgta tccattagcc ctcaactatt
     4081 catccacgct aaaaccgtgc gatctactcc actactttct agcaattcat tttgcatatt
     4141 atgagcgaat tatctgaaag gcaatttcaa cgtcgtagca aagacaaact atgagtgcaa
     4201 tattttatat gtgtgattat cagcagtata tctgctgtac caaaagtaca tgttaggcgg
     4261 aaacaatcag aaccttaaac cgcgttagtt cagggagttt cgcaggactt ccctatcgtt
     4321 agcagcgggg cacgggttct ggctagaatt ggaaatagcc taaaacctgc agagggtaaa
     4381 tccgacccac tttatatttt gcaaccatat gctgactggt gcggtttatt agccaa
//
LOCUS       DAOR01000118            6559 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_74851, whole genome shotgun sequence.
ACCESSION   DAOR01000118 DAOR01000000
VERSION     DAOR01000118.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_74851"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1771..1985
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5818..5945
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1929 a         1222 c         1064 g         2001 t
ORIGIN      
        1 agtcagagac atggcaacag atacagcgga gacattccct aaggcgatca tgacaaagct
       61 tcctgagtgg ccttaatgcc ataagttaca caagacacac tcttgtttta tacaattaga
      121 aatgtacaag gagcaaagcc atattttgca agagcatttg ccatcatctc agatttgtta
      181 ggtgcttggg cacgtattct ggcagtcctg cggactattt ttctagtaat tatttcgtta
      241 ccagggtttg ctggttaggg tgtttggcta tcttatagcg tcaactaatt tctactcttg
      301 ttcttgacgt tgctcgaggc tttggcctcg atgattctgc agatacagtt accatttatt
      361 ttcccttctc cagcacaata atagcgccac ctcatgcagt aaatatattc tattggccat
      421 tattcttatt ttataatatt ttctataata ccatagttca acttctaacc acatatcacc
      481 actaatttaa atactatttt tagtagtggg taatcactta caaaatggcg aggagaccaa
      541 cagatccaaa gataacaata attggggtcc caaccaattc atctggaaac attgatggtg
      601 tttcaagagg gctatcagcg cccagaaaga agatctgcaa gacagatcgt tcaatatctg
      661 gcagctgtta tgaggtcttg atgaatgaat aaaaaaaacc tacataacaa gatttttttg
      721 tcttatacat ttattacata caatacaact attacacaag acgtttataa gtgtaatgta
      781 atgaatgtat aacgtgtaat tgtattgatc aagcaacgga aggatactgt agagtatcgt
      841 aacgttcgtc tatctattga aacctacact aaacttgaca aatatcttct tgaacttatt
      901 cagaaaagag gtgatagacg actttcgctt gacgacgcaa taaagtcttt aattgaggat
      961 tactattcta agataactag agattaagga ggaaccttgc aacactatat aggctgtact
     1021 ggatggagct atacatcttg gaaaggcccc ttctacccat ctaatcttga aaacgctaaa
     1081 tggctgaact attattcaca gatatttgat tatgtagaga ttgattcttc gttctataga
     1141 actcctaacg tctttatggt taaaaattgg ttcaagcgta ctcccgaaaa ttttagattt
     1201 actgctaaat ttccaaaagt cataacccat gataaaagac tgaagaatgt cgatagagaa
     1261 ttggagcttt tctttcaagt aattgatcct ttaaaaagta agactttggc tttacttatt
     1321 cagttaccac cttcattaac gatatcccaa ggtcttgaag gactgaggca attattacca
     1381 accttagata acactggatt caggtatgca atagaagtta gacacaattc atggtttcaa
     1441 gatctagcat acaacttctt tactgataat aacatttgta tggtttggag tcagcttgca
     1501 aacatacgaa cgcctcctat agtaactaca gattttctat acataaggtt cattggcgat
     1561 agaagtatac aagaaaggga tttcggtagt atacagaaag ataggatttc tgaaatgcga
     1621 aaatgggcac gtaatcttaa agcagtacca aagcgagaaa caggagcaga ggaagaagga
     1681 gaagcaataa tggggagagg aagaaaaaat aatattaatc ttgcaatcgt agcggcaaat
     1741 aatcactatg ctggatttgg acctggaacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnntaaag caaagtacgc tttctgattt tatagattaa caaagaatac aatgaacaag
     2041 tagaaggagg tctttacatc ttttaattaa agaaaataat tctgtgcatg gtccttccta
     2101 tcattctctg agaacaagat agctatcagc tccctgaact ggagcgcatt aataatagct
     2161 tttctacagt aatgattgtt aaaatataca ctaaagaact tttgtctcat ttttaattcg
     2221 cgatattttt tcaactagga taaactgatg tcgaacggaa gcgtattagc tcattttgat
     2281 atcttttagt atctgatagg tctttatttc tccatcattt atcgaattct ttattgtagg
     2341 taatgaaaaa tctgcattct cgtaaaggga tggaataagc tctgttctag ttatatgatg
     2401 tagaccctta atttcagctc catgttcttc tgatactttt ttatatcttg cccggatctt
     2461 ttcaaacctc gctttatctt ctttttcttc tgaatcggta gatataagca ccatcctcat
     2521 cgcgactttt tcaaactgta accaacgatc atttgacaaa tctatttttg gatattatta
     2581 ttaatattat gaccgtcagc acgacatcta gctgctacta ctaatgtcta gcagaattct
     2641 cccatctctt tccttagcaa atagtgcttg taaggcttct ttagcttcat caagcgtgaa
     2701 ctttttccac actagtcttt aattctttag acatatctat gagctctgtg atctctcttc
     2761 tagtgccacc agtagaacct attaatttta cttgtttaga ataaagtgat tgaatattta
     2821 atttaacatc tacacctgtt agacctccaa aagttaccca tcttccattg aggccaacag
     2881 atgcaaagct attttcccat gtttgtaccc ctagagaatc taaaacaaca tctgccattt
     2941 tgccttgagt taattcatta actttctcta ctactctatc atattcactt ataatataat
     3001 cggctccaaa atcttttatc caattatctt tagagactgc aataactttt gctcccattt
     3061 tctttccaaa ctgtgtagcc atcatccctg tatttcccga tgcaccaaag attaccaagg
     3121 attcatttac ttttaacgca gcttctttta atgcatgata tggtgtcaaa gtggttacag
     3181 gtagactggc cgctaactcc cattgcattt cgtccggtat tttaaagaca tttttctccg
     3241 gaacagccat gtattctgca aagtctccat tggtagcaac acctatcagt cctccgtttc
     3301 tacataacat atcatttcca ctcagacaca tatcacatat tccatcaaac accttgttat
     3361 gcacaatcac tctttcacct ctctttaggc tcgtcacatg ccgtccagct tcttctacca
     3421 caccgcttgt ctccgctccc ggtatatgcg gtactggttt aattggcagt gtacctgaca
     3481 ccacaatgtg atctattgga tttacaactg cagctttaac tcttatcaac acatcgtgat
     3541 ctgttatttt cggttgttct acatcatgct ttatctttaa attctctaaa ccgggttttt
     3601 caaaaatggc tgccttcatc tatctattat gaagtatatt ttatttaaca tcttctgcag
     3661 tagatttaat tatttctgaa tacttgtgat ttaattcatt aattcttgtt ttgcatagat
     3721 ctgtttatcg tatgcctatg ctactgccaa aaagacatgt actcgcataa tcatctaaac
     3781 ctcgagaaag tagctgttaa acaggaaaga aggaagagac agaagccatt ctggcttttc
     3841 taatatgttc taaaattaaa tcgtgtccgc tgttttgcca atttattgat gaccaacttc
     3901 atgaaaactt acagtttaca gaccatattc atttaaccat cacaagacat tttaagtgac
     3961 aactccaaat gcaaacttca agcaacggtc ttttaaagcc tgacctttct cttgttataa
     4021 aagtaggtgt tgatactgct gctcgtcgct ttttcctacc aaactgccga aggtcgaaag
     4081 attttattag tatgttgttt tattcataat accgaggtac ttgatagaca tgaaagcgtg
     4141 tgagcatgct aggaatgttg atgaaaatct tgcacccaat acatctggct gtgaagaatg
     4201 tgaaaaagaa ggaacagagt gggtcgcact aagaatgtgt ctcagttgcg gtcacgtcgg
     4261 atgctgtgac tcatctgttg gtctgcatgc aactaaacac tttcagaaaa caagccaccc
     4321 ggtgatgata gcgcttccag ataaatcgtg gaaatggtgc tacgtcgata agaattacac
     4381 ccgttaagaa ggaatccaat tcaatctgga taccatactc tggctatctt gctgaagcac
     4441 ttcctgcaga acgcatttca aaacaatcgt cggtaaaccg tattatgaag ctagtaagaa
     4501 atttgtagga gatgcatatt tgtgtcctag ttgcaaggtt tgtctatgtt cgatggcagt
     4561 ttaatttaaa cgactataac gatcaataga ttttatgaat tcatgggtat ggatatccat
     4621 tgccttgatc tcatctgcct taatgtcaaa aggagtattt ttcttagtat tgaccatcat
     4681 ttcagtatca accgttatcc cttaaatcaa gtattatctg cgtcaatatg acaagtaaga
     4741 atgatataac cactaaagcc acagccttgc tggacatagg attgtatatt tggatatgtt
     4801 attaaacatt ttttaaaggc tttcaggacg tcctataagg aaggcctcag tacatctgca
     4861 tgatatattc tagtagtggt gctaaacgct ttccccattt attatcgatc tggccgtttg
     4921 aaagatatta atccacattt gccatgttaa tgtaccggtg ttagtatttc ttctacttgg
     4981 atgatatgaa actatcaaag tcttaccatc aaagacagga tagatcttat tatgaccaaa
     5041 catcaatcct ctaacgttgc aaacactgcg atatgcatca aaagcaatct ttccaagggt
     5101 caagataacc ttggttgtct tctcgagtat ttttgtttct gccaggaggt attgagaaca
     5161 gttggatatt tcagatggat ctggtttatt taatggcggg acacatcgta ctgctgcagt
     5221 cagataggca tcctttaaaa ttaatccatc gtctcttgat acacttgtag gtttatttgc
     5281 aaatcctgtt tcaaataatg ctcttataag ccaatcacca gagctatccc ctgtaaatac
     5341 tctccccgtc ctattccccc catgagcagc tggagccaat ccaataacca ataatttagc
     5401 tttaggatcc ccaaaagttg ggacaggtct tgcccaataa acttgattca taaacttctt
     5461 cgttttagtt tttctcactt ctcgaatgta ttttgataaa cgaggacata atttacaatg
     5521 tacaaccgtt tcatttaatt tgtgcaataa caaatcttct tcaactaact taccctttga
     5581 caatgccaga tattgtatat ctaaatgctg tatattaatt gcgtttgttg gcaaatacgt
     5641 gccttgagat accccgagta tcccagtatg gagaatagat atttacctgt atgatccaca
     5701 gttaatatag atactacctt gacaaataat ccagttgcag tctttgattc aggagtcggt
     5761 tcaatatcaa taattaaaga attaaaaaaa gagatcccgc atgaggacct gctttacnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnngacct gctttacttt gcagatagac ttcattttcc gtacggaaat aaatctcatc
     6001 agcagttgta cgagattatt ctagcgtcga taaaattttt agagaactac aaacccaagg
     6061 taattgtact agcatctatg accccctctg tccaaatatt acagaaagtc agaacacgag
     6121 tcaatacccc aataatcggt gtaatgcccc ctttaagagg aacagcaagg ttatcaaaga
     6181 aaaaacacat aggcattatg gcaaccgaag gaaccgtgtc cagtcaggag ctagctgcgc
     6241 tgatacgaaa agagatagct caagatgtac gcgtaaccaa gttcaatgct tcaccaataa
     6301 tagagctgat agaaaatggt atttacatca ttaatgaaag aaaaacattt gatactatat
     6361 cacacgtact aatgggaaag ctagaaaagg aagatataga tgtaataatt ctatgcagca
     6421 ctcatctttc attggtacga aattattttg gttctctatt tccactcgta aaatttatag
     6481 atccctccaa tattgtagcc aaagacgtta aaaaattcct tcgtcataat agaatgctta
     6541 ggaggagcgg aataggtct
//
LOCUS       DAOR01000119           14154 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_12930, whole genome shotgun sequence.
ACCESSION   DAOR01000119 DAOR01000000
VERSION     DAOR01000119.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14154)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14154
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_12930"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    8823..8926
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4255 a         2620 c         2765 g         4410 t
ORIGIN      
        1 cgagaaggag tataatctaa atgcaagcac agcagaacga tttgtcaggg aaaaatgtga
       61 agtcgatgtc tctgacagag aacgccatga aatctgtaga gacctttact atgcttacgt
      121 agtatactgt aaagagaaca gtattactgc caaggctgat aacgttttcg gaatggaact
      181 ggctcagcag catataaaga aggaacgcaa actcgtaaag agacagaggg aatattgtta
      241 cgttggaatc aatctcataa acgcaatgtg actatgggtt ttcatctact accaaattct
      301 tgatatatta tcctgaagac tctgtcaaag tctttttagt tactgcccag gatgtctagg
      361 ttaccatcta cctattttcc tgtggttaag tgtacatatt gaacggtaat gttagtatag
      421 acataataat agaaaagaaa gttgctggca acatagacat cttggacatg gacgttgcaa
      481 ctttaacact tcgctggttc caggacagaa tctgactcat tttgtcctcg agtctccgtc
      541 atcacttgac tgtagtccac ggagttcgtt accggatatt ggttttggaa aaagccattg
      601 cttcttctcc ttagcaattt tttgaacaat atagaatgga tttgatgtgt tgatgtatat
      661 tattctccct tcttttaata taatccgtac cacgttcgtt tagtttttct cttttttcga
      721 cattaggcgg ctgactgaca cagcacttga agcctttgtt ttgcttcttc cggatgcttt
      781 aggtagtata cgtacgtgat tgtactagaa caggggaagt caaaggaggt gctgacaact
      841 atggcttttt ctagttgttc ctcagttgct aggcttgaca acgtactccg gatagcattt
      901 ggtgcaatct ccattattgt aggccttgca gcaggcaaga agcggattgg attgttcaag
      961 gctcctagtt ggaccaggac tgtatctggt tcgctggttc taggtttggc ggcatatcag
     1021 catttcaaat gtcctcctga ggaggtgcgg cacttttcaa actaggtgct gctgttgcac
     1081 ctcctcgtga cgctggaata tttatcaaac ttcaatcatt tcgatagtct ttgcgatact
     1141 agccactatt gcgtactatg ctcatgaaag gatatggata agataaagtg gaagtagagc
     1201 aaggcgaaaa gagacaatct tgatgttaca attccgaaga atttctcgag attgctaaga
     1261 gagtactagt aagatatgat acgaagagta atacgtttta cttaaatggt atttcctaag
     1321 gtaaatcaac aagtggcttt tgcaatacct gttcgaatgg ctataaatgc ggtggtaatc
     1381 tatggcctat atgtggcacg cgtcgaatta ttctttggga agaaggtgtt ataggaatta
     1441 ccaatagatt ggattaagaa ccgtcactgt tatgggcttg gctgggtcgt acaataatat
     1501 tagtgataag ctaaagcagt gattgaaaaa caaaaccgta tgaagaaaag tacgctgtta
     1561 aggatcggta ttatcgtctt tgttgtcata gctgcaatta ctctaattta taattactat
     1621 ttaatctcac ctactactcg ttatgcaggc gtagacaaat tcgggataaa ggaaatctat
     1681 ccaacaaaaa cgggtgggga agaatggttt atgaatatgg atgatcctgg aaatgatcca
     1741 agaataggag gagaaggacc accaagcaag ttcgtgcaac aaaatgatga cggtagttgg
     1801 aaagtacgaa gtactgaagt gcgttacggc gtgttaactt catcaggtta tcatccagat
     1861 ctgattacca ctctcaatca gcaagccctg gctgcaaagg gatacatgca atcacccaat
     1921 gattggaaga acgtagaaat gactggatat tttaaggtaa atagttttac tgactccaat
     1981 cagaacggtg gaccacatat agagttactt gcacggggtg gaagaaatac aaacgacatt
     2041 ggaacaatag atggattgtc aagacaatgt gaagcaacca cttatcattc aaatacatat
     2101 gtagatggca gagttaagtt tgagaaagat ttggagcata cgataggata tacaaccgga
     2161 gatcctgaga agcagcatgg tataagccct ttggttggaa ggtggattgg agtcaaagca
     2221 gtattctata atttacctga tggttcagta aggctagagc aatggatcga tgagaattcg
     2281 aataataact ggcatcgagt tctcaaatat acggatgacg gtaactgggg tggaggaact
     2341 cctaactgcg gcggccctga tactcagata ataacatggg gaggtccaat tacaatattc
     2401 aggtgggata acattgatga aatggatgtg aaatacttta gtgtaagaga aattaagcct
     2461 ccttcttaag tgaatataaa gtattacctc ctgctgccaa ccatgtctac tttagcttag
     2521 aattggtatt ctttcacgtt ttttaatacc gagatgctca caatgtctaa tcgtacggct
     2581 catttctcct ccagtttttg ttatcgcaat tatgtaatac aattccaatt tgcctcttga
     2641 cttaatcaca atgatctaaa aggagagatt gtgatcaata cctgatagta ttcttgtttc
     2701 ttgcttctct ttctttaagt ggttactccc tcagccaata gtgactgaat catttgctca
     2761 gtttgatcat aatcgctata tttgaaaaag acctctgaaa gaggatggtc gtttttcgtc
     2821 tcttctctta ataacggcat cccaattctg tgtgtacctt tgggtaacgt cttccacact
     2881 atcgttagaa aatgcaaaga catactcata ctttttcccc caattctgct cataatagtg
     2941 aggatgatct agcgagattg gatattccaa agttgaatcc atatgaaccc attttccttt
     3001 tagcagagct tcgttccaac agtgatcaag gaaatcatgt acaatccttg tctctatttt
     3061 tgatacgttc atgacagcac caaaaagcat cgaccattcg ctacatctgc cagttcttgc
     3121 ttctgcaatc ttcaagatct gaccatgtct aggaaaagaa tattcatagc tgcatttgtc
     3181 acacctgtgg atttccattg cgcgtagctt ccatgaattt ccaggcacta tccgaacatg
     3241 catcaagttc ccgcgacctt tatccataca tctattccat cttggatctt ttggagtcca
     3301 actcataaag tcatttttga accatcccat tgtagatttg attttatcgt cctcatttag
     3361 tcgtgctatg tgttctggta tcaacgattt gattttttct agaatctgaa aatcatagta
     3421 ctgttcgcca attttcttgt agagtaggaa agtgctcttg ttgatggttt gactaaggtt
     3481 gccattcaga gtgatatgac ctccatacac gtctctagtt atactgatta tatgcttaaa
     3541 aatggaaaaa tatatttgtt cacaaatctg tcacctgtgt cggttggttg gccgtatgtc
     3601 agtagccaaa attgtagagc ttgtgagctg gtgagctggg tgggtagttc tgaaaaaggc
     3661 tgggagaatg cagcacagac tgcagttaga gaggaggcca agacgataca tggaattcgg
     3721 ggaatagaag taaaagaaat agctgcaaaa atagaaggca aaacaggaaa aatacaaaag
     3781 ttcagaggcg gtattaagtt agcctatggc gtagacagaa aatattgtag atatatccaa
     3841 tagccatatg gaaattcaaa aataatgacg aaaggcaaac aacagcttga cagtaaatga
     3901 aaaagagatt tatagagcaa gcgatctctc gacctaatgg gttacggtag aagttacggc
     3961 ggtggcggca gtagagactt tagaggatct acaggcccaa aaccagtaga agtaggtaag
     4021 gaatatgaca tagaagttac ggaaatcagt agacaaggtg acggtatcgc aagagtccaa
     4081 ggatttgttg tttttgtcaa aaatggaaaa gttggacaga aagttaaggt taaggttgat
     4141 caggtaggaa ataggttcgc tacagctacg gttgtgagct aaatcacgca ataactgagc
     4201 cgtacgtgga cagttggttg gacgtgtcat ggttgcggta accaagatac ggttgttata
     4261 cctgacacaa tatcgagatt tggaacgaca aaaacaaatg tttcctgact atcaagctaa
     4321 aaagtatagc tcataacttt cagcttgctt tgcgtaacca gtcctgcact actaggtaat
     4381 agtatgtgac tggatgacaa atactatagt tgcactatag tattgcattt tttcccaatt
     4441 tctttacata agaatgtaaa taaactagta tcgctctttc aaaatcacat tatgtccaga
     4501 aaaatattga cgcccgtctc tagtgcttta gccctgctta tgcttctcgt gacatcagca
     4561 actttttctt gtccagtgtt agctcaaccc ctattcggta acagtcatgg cggtgcgatg
     4621 atttttggtt caccgaattt catgaatagt atattcaatg catgatcaat ctcaggtatt
     4681 gtaggtataa gcatggtaaa tggagtcaaa gttaccggag aaaaccttgg aaataatgag
     4741 ataactgtaa ctctaaaaca cgtaacaaca agtagtaata gtagccttgt accttcagtt
     4801 acagttatag cgctaagatt acctatggat ctcaaaggtc taatgtcatt agctgtagct
     4861 tcacgcagta cgggaggcaa taatactagc atgttgatga ctaacaacac aaatcccatg
     4921 aacgctatga tgggacaagg gtttagagga ttaggagcga atgctacaac tcagaataat
     4981 ccgttcaaag cgcttggctt tttgaggaat attcagatag gaagtagcag gatagttaac
     5041 gctgattaga gattacctca aaccactaca atgggtttga ttggcggtag cagtacgtct
     5101 accgcttctt catctgctga tttcgtaata atcacagtaa ttccatatac aggcaaaact
     5161 agcaatctga gtggatagaa aggaaagcgc tatccttaca ttcagggaga gaattgtcat
     5221 aagcaatgac atccatcttc ttctcgatac cataaattcg accggtcgtt gtaggacgtt
     5281 ttataattta cgacaggcat gttttaattt agctttcgcc tcttactctc tgtatgtttc
     5341 tcagcattga atctttccag ctcatacgtg cctaagtcta caaacttaat tcctccagca
     5401 agcacaggat ggacacgttt gatctctgta aaaatcttct ggcatactct gcgataatct
     5461 gggtgccctt gcggaatagt tctcagctca atcatatggc atgcctctcg caaattaaat
     5521 ttcataaaat atggatattt gtatgcaaaa ttaactacat attgtgattc ttcaggcatt
     5581 ttcattgaaa tgattttata tacttcatca catttataca tacaatcttt gaaatcttta
     5641 tcgatatcca tgtcgataac ctctggtgga agatcatatc catgtttagt agaaagcagc
     5701 tgtctttcta aggtaagtat tctatgacga tgcaggtcac ggaatattcc gaaatttgta
     5761 aacatttcaa atgtaaattc taccatttca aatgctctac caggacggtg tcttctatta
     5821 gctcggaatt ttgtataggt ttgaattatt ttatgccttt ctattggggg tagagatcga
     5881 atatatcgtg caagactctc aagagactga ccttgggctt gctcatataa aattgcagag
     5941 ccaactttta tctcagcttc agcattatct tcaaaattta gaagtttcac agattgtggt
     6001 ttttcttccg ccttgagatt ttttgtgtga tacctagcaa gctctaagat tgcatgcttt
     6061 gtatctataa gatatgtctg tagagcatgc ccgtactcat cgtttgcacg tcttacaaaa
     6121 gcaaaaataa ctgcatcaag ttctgattgc aactgaactg caagcgattt tatttcagtt
     6181 aggtctgatg agaacatttt tgatagcaag tattcaaatg ctcgaccatt gcctgtgatg
     6241 cccaaatttg taagagtgga ggctggcaaa agtccacgga ggacatcaag tgccttggcc
     6301 ttaatagttg cattatatat tcgtcttgca gattcaatgt ccttgtcaaa tctcaagtta
     6361 ctataagcta cttctcgttt tgacaacgag tcgaaaaaaa gaaagcggtc gattggctcc
     6421 acttctgcaa taaatttctg cattggttgt aaattcttgg tgtaaatgtc gaaagcatga
     6481 tcgcatgcct gtgtgtataa atcagcatgt ggtgatgcca tgatgcgctc ttctttaaag
     6541 tacttgtatt gaccattaac tttttgatca aatgccacat aacgtgaaga tttttcaaga
     6601 tatgaaagtc cgattctatg atcctcgatt ttcttagcag cgatgtttga gatccattct
     6661 attgcaatct gtgcctctcc tagttcagca acagagtcgt ctccatactc taacaatact
     6721 cttccataga attcctctcc cctgtttgga tttttggcaa attcgtctag gaatatgcgt
     6781 cgcattgtct tatctgtcct agaatatctg gacattaatg ctcctctgtc tacctgtgca
     6841 ggcgtaacta ttgcaaaaac aggtctatca aggttagaaa aatgtgaacc tagaataaac
     6901 ttttcttctt cagagaaatt atcatagaaa attgtgttat aagagctcat gttgcgtaac
     6961 tcatgacgct gactgataaa aactttgcat atttagtaca gtttatgccg cacaatataa
     7021 ttgtgcagat acttcaatcg ataaattggg tttgcattaa tcaatcaaga tacatttgca
     7081 ggttaaagca ttaagatgaa gacaccagtt attttctgaa catgaacatg cattgaacca
     7141 caccgttcat ccgtccctct atccgtttca atcggatagt gattgatacg atctggttta
     7201 acgataaaga atggaaaagg atcaaaaaat acacagacac ctcacgacgc acttgctacg
     7261 ttaactttgg gtgctcatct tttctttacc gtgcaacggt attattgcag attgtcggac
     7321 cacctatagt caaagacggt aggtagtgcg tatgtcacca atggaccacc tttcctttaa
     7381 caatctatta ccatattttg ccaaatcctg gtagagagat tgggaatcta aatcataagg
     7441 tgaatatcaa gcctgatggt atttgctatt tagttattag cttgacgtca gagtggtata
     7501 acttcacatt agcgtcttcc tttacttctt tagatactcc ccacagattg gggtgctatt
     7561 ttggccttca ggatagttcc tctcctttcc tcacgtcttt gttgtccgta taccttccgt
     7621 atctctacgt cacccattcc aatgcatttt ctggtcatca ttacccttga ctataatttt
     7681 cttgggtctt tcacaaatcg catgtgattc ctatcttggc tatagattgg atgctccacg
     7741 tggctgtgat tgtggttgtg gagtttgctg gccttcctcc aaggaggaat gccagctacg
     7801 gacatccttt tcctaacttt tacgaccttc agtccgtcta cgttcatctt tgcgtgcttt
     7861 gaactccaca tctttagtct tccttctggc tcttactccc gctattccct ccaaaaggaa
     7921 ccctaattcg gcttttctgg aattcgttaa gatgcctaaa atcctctttt atcaccatcc
     7981 ttttcgggca aattggcctt actaaataat gcaacaattt tgacaccgtc accattatac
     8041 gtacttacaa aatgatttgc atgagcgaac tcaagctcga attcgtgggt aaacaagtca
     8101 aggatatgta tggtacattc atgggaaaag tggttggtat aataacagat actgatggtt
     8161 ctattgaatc tgtcggcgta gattgcggtt ctgcaggttt aaaacagcta ccgtatgaac
     8221 agctattagt tcaaggagat tatgtaatct atattcccag gtggagattg gacgctcaaa
     8281 agctattgag gcagaaaaac cttactctac aacgaataaa gggactacag gatatcgttg
     8341 cagaaaatga tttgatgaag gacgatgctg aattggttta cctgaaatac gaaaagaggc
     8401 tcaatgatct tggtgagatt gaaaaatctg ttaatgagaa gttgcaagca agattgaatg
     8461 aattggatac agaaaacaga agcatcaaaa cggtgttgtt tgatgcgaag ctacagttca
     8521 gaagcaatga gataacggag gaggcatatc aacaaatcag tacgcacaca aatgaactaa
     8581 tagagcatat tactatcgaa aagtctgaaa tcaataatgt taagatgagg ctcacacaac
     8641 aaacgttgga aaatattgct ttaacgccaa gactacctac tactatgtcc acatccaata
     8701 caaaagcagc tactggagaa tcatctcaac agcaactaga atctacaccg actatatcaa
     8761 cgccacgcct cgtaaatgcg gatgctactg aatatagtgc aactgataat accgcaactg
     8821 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgca actgataata
     8941 ccgcaactga aaattctagt gagggtaaaa taataacgaa aactactaaa cgtgaatcag
     9001 attcagacgc aagttggctg aaccaggtaa ttcaaaacga ataaattttt caacgctata
     9061 caaacaacgc tatacaaaca aagaaaaaga gaaaaatgta agaaaaaagg tattatttat
     9121 cctattttcc ttggcttgtt gaagtctgca aaagttcgcc tatctcgttg tgcatcttta
     9181 ggaaatttaa tcccttctcc gttgttttgt acgtctggga cccctccaag tactctaaca
     9241 aagtgttctc gatgagtacg gaaagatatt cccgtagctg tgcatagcta agaaaggcct
     9301 tgtacatgat ttttgtcttt gtagccccgc cgtttgctgc ctctaggatc atcgctacta
     9361 tttctgttct acttctgtat ttcacaatta aaatacactg gatttgttta ttttaatata
     9421 ttgacacatt ttgtaataac aaggttccgg ataaacatta catttttatc tgtgaaatct
     9481 taagaatacc aataaagaag cacttttagt atataagttg tgctagatca caatggtcta
     9541 gagattgttt tacgcacaag catatccaac caaacacttt aagaattgtt tatacttttc
     9601 ttatcactga tgactgatca aaattttatc gatttatcta ggatatatga ggagagtgaa
     9661 aaagtcaaag taagcttaca gggaaaaaaa ctagataaaa tgagaagtca agctttggaa
     9721 gaccctgagc aattctgggg agagcaagcg aaaaatctca tttggtttaa acagtcggac
     9781 aaagtattag actcgaacga ccatccattc tacaaatggt tcgttggtgg tgcattaaat
     9841 gcatctgtca attgtcttga tagacacgta gattctgatt taaaaaacaa ggctgccata
     9901 atctgggaag ctgaaaataa ggaaagcaga tcatttacct attctcaact atatcgttct
     9961 gttaacaagt ttgccagtgc cctaaaaaat ctcggagtca agaaaggtga ccgtgttaca
    10021 atttatcttc ccatggtgcc agaacttcca atcgctatgt tggcatgtgc aagaattggg
    10081 gcaatacata ccgttgtgtt ctctggcttc agcgcgcagg cattatccga tagagtaaat
    10141 gactcaaatt cgagagtggt aataactgcc gatgcaggct ttagaagggg aaaacttgtt
    10201 gaattgaaaa aagttgtaga cgaagcgatt cctctttctt cttcaatcga acatgttgtc
    10261 gtcttgaaaa gagcattaaa taacattacg atgggtccaa aagatttatg gtggcacgag
    10321 ataattgaag atgcccgacc atattgtgag ccagaaatta tagagagtac gcacccgttg
    10381 tatattctat atacgtccgg tacgacaggc aagcctaaag gtgtcgtaca cagcactggt
    10441 ggctatttga cccatctata cgcaacagct aaatgggttt ttgattttac aaacaaagac
    10501 attttctttt gtactgctga tattggatgg gtaacaggtc atagctacat cgtttatgcg
    10561 ccgctaatgc atggtgttac ccagataatg tatgaaggag cgccagatta tccgaaacct
    10621 gatagatatt gggatatagt tgaaaagcac ggcgccacaa ttctatatac cactcctact
    10681 gctcttcgaa tgtatattaa attcggaaat agtattccta attcttttga tttatcctca
    10741 ctacgtctat tgggtaccgt tggtgagccg ataaatccag aggtatggat gtggtatttt
    10801 agaactattg gaaaggaaaa ttgtcctatt gttgatacat ggtggcaaac tgaaacagga
    10861 ggcatcatga taagtgcgtg tcctggcatt gaaatcgtcc caatgaaacc cggttctggt
    10921 accttttcct tgcccggaat agatgcagtc gttgtagatg agaacggaaa accagtaacc
    10981 ccagacaaaa aaggattctt ggtagttaga aaaccatggc caggaatgct gatgacactt
    11041 tggagagatg atgaaaaata ccagaacacc tattggagaa aattcgacaa caacatgtac
    11101 tttactggcg actacgcact ttatgatacc gatggatatc tttggttgct aggcagatcg
    11161 gatgatgtac ttaagatagc aggacacagg ttgggtacaa tggagttgga aagtgcgttt
    11221 gtctcacacc gagcaattgc ggaagcagca gtgaccagta ggccagatga gaaaaaagga
    11281 gaattgataa ttgcattttt ggtattgcgt acagaattcg cgacagtatc tgaacaacag
    11341 ttacgaaatg aacttgttaa tcatattaga gagacagtag ggccaattgc aacacctggt
    11401 gaaatttatt ttgtcaataa gcttccaaaa actagaagcg gaaaaataat gcgtagatta
    11461 ctgaaatcga tatcaagcgg gtcatcgaca ataggcgatg taagtacatt ggaggatgga
    11521 acatcaatag aagaagtaag gcaagcttat gatgatttgc ataaagcgat tggttaacgg
    11581 agcaatgata ggaggctgac ttatcattat aactactcat ttggttaata gtacagtatg
    11641 atttttagat ttccaatcgc taagtaaatc aaatccacaa gtatttttta aactttttct
    11701 taatattaac caagtatccg aaatgatttt tttcatcttt ctgtccctaa ttggtagatt
    11761 aaatacgaat gtagctcccg tgctattacc gatgatatcg cctttgttgt tttctgccca
    11821 tatcctgcca ccatgtgcct cgattatgct ttttgatatg aaaagaccca atccagtgct
    11881 tcctgtaata gattttgtgg caaattttgt aaatagccta gataatattt caggatgcat
    11941 gccaggcccc gtatccttta tacagactat gagttggcca tcccttgcct ctgatgtgat
    12001 ggttatactg ccgtgttttg tgaattgaac agcattactt agaagattag aaaaaacttg
    12061 agtcattctc cctttgtctg cttctactaa aatatcttgg ggatcgtata ataaccgtac
    12121 attttcaccg ttaaagtctc tacttaaaat catatcatcc atagaattta ttattacgtc
    12181 gtttagattg aacccttctt tctttagttc taatgactac tttcaatttt tgtagcatct
    12241 aatatatcgt ctgtaagccg actcaatctt tttgcatttc tattaatagt atagagtaat
    12301 tcattgtact cctcaatgtt tcctgtcttc gaaagaagaa gttgcgacaa accaactatg
    12361 ggttgaatag acgttcttaa ctcgtgtgct gcttgttgat aaactctttc tgagtcttag
    12421 catggacttc taattgttca tgtgatttct tgatatttgc atataatttc gtttgtttcc
    12481 ataaattctc aaatatggca acgtaagaca aaactccagg tttactatta taatatgttg
    12541 ataatcatat tgcttcatca aaagtgtttc ttgaatcatc tcttatttcc attactaatg
    12601 aaacgtttct atcgactact aggattgtga tttgagtacc ggtatattcg acaatactat
    12661 gtgctgaatt gggtagaaaa tgatataaag ctcatagttg attttaaacg aaaataaacg
    12721 cagagagtag acttttttgt ttattcagtt cctgcttcat ttttttcttt atcgatttcg
    12781 tatcaattat cagagactat tatcttacgt gtccaaaaac cggtggtgct accgtatggt
    12841 ctggtcttgg tcaacgtcgc aggctagaat catctgcacg ataatcgtat gataaaaatg
    12901 gtggttattt ggcaactttt tgactgatta gcctcttttt atatttatag tcatttatta
    12961 ctagattatt ttttgctcat ctaatggata aagataaaca ttggcccttt catcatcaat
    13021 ttcattagaa tgtatcaata tggttccttc tacttcattt tcatctaaac caattccttt
    13081 cttccctagt atcgaactag gatctatctg attaggcaat agtccattcc ctggatatgg
    13141 ttctaatgtt actctttctt cacctaaaag tcctgcatcc ctaaccccat tagacatcat
    13201 tctgcctcca tcatttaaca tgatatctac attgtatttc tttcttaaag tcaacatagc
    13261 tctatagata acaaattctt ctccagttat aatatagttt acgagatcgg attcaggtat
    13321 tgtcttgaca gcatagtcat atccactttg agaagttaca aatattacct ttattttgtt
    13381 atttggatta agaacagcta tctcattttt cttatattct tccagtaagt tcactcgttt
    13441 gaaggtttta tcagagcgag ctattacaat atagtaaaaa ttctttgacc tttttttaat
    13501 tagttctagt ttaagtaaat ccttataaat atctagatta gattctttaa agttattccc
    13561 aaagaactgc caattccata tctgattctt gtttgccatt aaattaactg cactcaccat
    13621 tattgcctta tatcctatca atattctatt aacagaagca aaagcattgt caccggattt
    13681 tctcttacct ccagacaatt gtaccacgcc tccagcattc ttgcctggaa ttttgtaaat
    13741 gtacgaattg tcaggagctt ttgtgtttgc tgcaatatag cttgttgcct ggtaaggaac
    13801 tttcattcta ttaatataat catcggatgc acctataacg attcttggca gcatttttcc
    13861 taaggatttt aagtcaaagt cgttggttgg agatatactc tgaattcctc ttcttatgaa
    13921 atgatctaat tgattttgtg taatcttctt ggcgtcagca agcgacttta agctctctcg
    13981 atactcatat tcaaagctat tattctgcat aattaatatt attatgacga tatattccct
    14041 ataaataata gcgatttgta tagactacaa tttgaaaagc attgcaacta caatcagtga
    14101 cggccctaaa aaagggaata gtggaaggag tggtttacta atcctagagg gcga
//
LOCUS       DAOR01000120            8118 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_82763, whole genome shotgun sequence.
ACCESSION   DAOR01000120 DAOR01000000
VERSION     DAOR01000120.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_82763"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4004..4176
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4609..4734
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5647..5771
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6188..6364
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2598 a         1327 c         1598 g         1994 t
ORIGIN      
        1 aaaccagata gcaagttctg tgctaagtgt agaatggtat tgacgtatga cgcatataat
       61 gaaactttgg agcagcaaga gcaaaaggaa tcagaagtta aagaattgaa ggcgaaatat
      121 gaacaagaca tgaaagctat gcgtgaagaa atggaaaata gattccaaca gattcttgcg
      181 aagatagatg ttcagaaaat aggctagttt aacctagctc agaatctact cattaactca
      241 ttataaaagg taagtgcttc ttttttattt tctaaaattg aactcaaata cgaccatttg
      301 ggttctcctt cagtcttaac gtccctcact cgggcttccc aatcctcaat atctgacttt
      361 agcttatcga catttcgccg aaaatctgat ttatgtgggt ctagtaaaga aaaaagagct
      421 gttataattc ctttcacttg gttcctcgta aatctattat tgtacacgtg atagtctgag
      481 attttagcag gcaatgccat tagtaatgga acatggttgt cgccgattat tggtaaccaa
      541 ccctcattgt tgagattata tactacacta tctacatgtc gagatattgc atccgtaatg
      601 attttgttta agattttttt cacaatattg ttagtctgga attgatgtaa tattcccgca
      661 gatctaaaca ataagagatc atttaaaggg agcgagcatt cgaattctaa aaggcgtatt
      721 ccctctgcgt acgaattgta gaactgtata tcttgtgttg accatccttc acttctgagc
      781 ttgccctcca ggagctttgg tgaattattc tttcgaaaat taaaggtttc tactctcaat
      841 ctttttaggt ctgagtagga cttgttgtac tctgatactt tttttattaa atctgccagt
      901 tcctgtccta aatatgtcag gcgtttgggc tttgcttgtt tatgtttacg gttcgtcgta
      961 taattggatt cctcgattat cttttctctc tgcaaattct tgatcacttc tatgacgtaa
     1021 gtcctatccg aactggtctg ttttatgatc tgattaacat tagtggcgcc tcttgaaagc
     1081 atgctcaaaa tcctcattga atagtcaacc agtctgttgt cgctgccttt cataaccggt
     1141 gtctaacttt ctaccaaaca tattagaatg ttataataaa tatgcattat gagtaagaag
     1201 cgcatttatg cactccagcc gtatgaggta gggaataaaa atgcaaaatc gctcgcagtt
     1261 gtgattccat ctgaggtggc caaggagaat aatgtaaaca catctactat attcacttta
     1321 cttgtagact ctaagaccaa aaaagtaatc ctgcaaacta tagataaaat atctgaacca
     1381 catcgaggtg atgcagtacc cgctggcgag agtttagagg cttccagcca gcaggcatca
     1441 tcaagcggaa ttcaataggt tgtgacgtcc atatgaaccc cataaaacaa gggactcctc
     1501 aggaatataa agagtgtgca ggaaagggct gtcaaaatgc tggtagctac catctgaaga
     1561 tcatttacct aaacaaaagt ggatggttct gcgaacggtg caggaacgat ttgacggtaa
     1621 gtggtctagc taaggggccg ggccttggca gtggtgaaaa tatttggcaa aaatagacct
     1681 agacctgata aatcaacaag gctatggtag acctgtcccc aaggagatgg atacccatgt
     1741 tatcaaatat gtaatcgggc aacgaataga cgccgtaaaa atagattcgg gtttactgca
     1801 gtggcaaaga tggaatcact aaggttttct atgatagtag gacgcatctc aatcgaaatg
     1861 gttgggacac ttcagtatat agtgatactg ctaaaggtgg aaaggagcga agaagtgaat
     1921 tctatagcaa gattaaggat gtgtgtgaga aattttatgg cgtaaagcgt catgagatag
     1981 gcatctttcc taacgacagg gctactatga cgttcatagg tcaagaacac gccgtgagct
     2041 ttcatagcct accacaatta atgcataatg gcactgatat tgtattcgta gaaaaacaaa
     2101 gcactgtcac tttgatgtca ccatacacag aaactgtagg tatatccttc attgattcag
     2161 agggctttgg cagtgaatat ggaattgctt tggcccggct atgtgatatg caaacccgat
     2221 gtgcatatga atatacatca gcagggaatg gtaaatacta cttccccaaa catcgggggc
     2281 atctagccaa tataaccgat tgtgacgtta gtggggttgc catagggatc aaggttaatg
     2341 gttctaaccg tttagggcta gaccttggaa gtattgagga gataaataac gtaaatccag
     2401 gcctagacct aactattgag gatctacaag aagacataga caccagcaga aatacacatt
     2461 atctaggact tttagggata ttcaaaggca aaggaaaact tcatgatagt ctcactgaat
     2521 ccgaaagata tcattacaaa tactaccttt cgaaacgatt cgaggttgac ggtgaagaga
     2581 ttcgatttat agattggttg gcgaaccata ggatagaatt agatactgta ttagcggtag
     2641 ccgaaccaga aggattttgg aattggctta aaaggaaatt agtgagtgtt tggcctgaaa
     2701 gaaactacaa tcgcgcaata ttcctgaacg attccctaaa tacacccact atggacaagt
     2761 ttatcgaatg gcatcaaaat ctaacaaggt cagttatagc ggacaaagtt aaagagaaaa
     2821 aagatcatct ctcgaaggtt gaaggcttgt atgatgacat agaatctgaa caagaaaata
     2881 ttgaggataa cataatcaat aacactctac tgaccaatga acaagttcag aatgtagacc
     2941 tagccctgga agggatcatg agcaatgatg atggtaaaac taactaacta tcatcttagt
     3001 tctcctatgc aagtgttaca ctgcctgcat gtgttgtcaa ataacaaaaa gttacataca
     3061 atcgtaatcg atatgcagaa ctatcaaacg ctaagaaatt taggacgtac aggggaaagc
     3121 ttcaacacag taatcaaccg attgatcaat tcgacaaaaa ggcagagttt ccagaatgcc
     3181 aacaacgaag ggggtaacga gatatgagtt tccttcccat gcccagctat tctgaacgtg
     3241 ataggaaatt cctagaagag tgcgcaaaac taggagatgt caatggtgct gtgatagtca
     3301 gtagaggcga cgttgacgaa tgtgattatg acgagctgaa agaaggagaa gatttccgat
     3361 gaaatgaata tttcatctaa gtcgctagga tgggtgaata gatgactgca gccactcaaa
     3421 ctcccgaaga gcggtctagc aataacgacc tgataccgtt agaacagatt caaaaatatg
     3481 taaatgaaaa cattccgatt ctaccactag gaccagatgg taaacaggat gtgcgcaatc
     3541 tatttacaaa agaagaactc gactcgctac gttcagatcc aaatatacta ttagaagggg
     3601 agaagaagga agttttctat gtagaaagag attacaagag tggtaaggac ataccgaaag
     3661 tacattatct gttgctactt tcaaaattta taccaagaga attttggacc acagaaagga
     3721 ttaaacgtca aatctgggtt ggcatcgcat ttctaacagg acctacaaaa attcctgcat
     3781 cttcagatcc aaacaaaaaa ctctgggggg ttggagtcga tgccgacgat gataaaacta
     3841 gatatgtttt agaaaatcta gtagacgaga aggatctcaa aagaaaagcc atagtccaga
     3901 cgacaccaca caaaggaatg cacattatat tttttgttgc agtaaatcca actagcaatg
     3961 aagaagtcaa ccgttggcgt aacagggctt tgtcattaag accnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacct ttgtcattaa gactttgcaa
     4201 ggattgtaaa attgagatta aaacggccac gatgcaatta acattagatc cttccaaaca
     4261 tagaatcgat aagacgttag gctacacccg tattagtgat ggaataaaaa tttgggaaag
     4321 tgactggctt tatgacttgt taattgaacg tttgataaat caaggatgtc ttaaatgctc
     4381 acccgaggaa taccacacca actaccaaat agaagcagaa caagattcaa aatatcttga
     4441 ggagatcgct aatcgaagag acgaggatag agaggaattt accgaaaccc agatgcaaaa
     4501 gggaatcgaa atattcctag gagaagacga agagaataaa aaagaaaata ggctctttga
     4561 cacgattttc cgacgaggca ctaaacatga cgtattgatg tattggggnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtgcc
     4741 cattgctatt tccataatat cactttggaa cacgcaaagt tattcgctca gaaactcgac
     4801 aatgctacag gtaacccgga taatccatta cgactagaac ccatagaaga ggcgtataga
     4861 agaggacgga atagacagtc gattcgtgga aaaagtggat tcatagaagc attttcagca
     4921 gcctgtaaaa atggtcctaa tcgaacttta gcacgacaaa ggttagaaaa attaaatgtt
     4981 gttctagact tggaagataa aaaacctaag aaaaaaggtg gtagtagtag caaagaaaga
     5041 aacgcaccaa atcaagctga tgtaattgta aagttagcgt tagaaaacat tccattttgt
     5101 ttcaagaacc agtttaaaga gtattgtgca gttgtcaaag tggtaactgc aacagaaaca
     5161 cattatgagg ttatgaatat ggaggatcat aacttttctt cagcgctccg atacttgtgg
     5221 gaacaagaaa atattgcgca aaatagacct cttaagacaa ctgtaagtca agaccacata
     5281 aaacaagcgc gtgccacatt agagagtaag attgatcata gcggcgttcc acgtataaaa
     5341 actcatttaa gagttgcatg gaaagaagaa aacaaaatca tacgttacga tctcagcaac
     5401 gacagatggc aacaggttga gataagtggc tcggaagacg gtaagagcgg agttagaatt
     5461 gtcgattcgg ataatatgat caaagaaata gaagaatgga agaaatgtaa attcgatcct
     5521 aacaaaatac ccgttctatt taggcgatac gagtcaaacg actctcaggt attaccagac
     5581 atgaatttcg agtcagacgt atacgacaat ttcatactta atttgacaaa tgtaataggt
     5641 aataaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn ngtaataggt aataaaagcg ccgacatggc aaagaaacaa gtagaacatt
     5821 tcaaaacaga aggcagtctt cttgaagata ccaaaaagtt gcttgctaaa gttgactcgc
     5881 atataaaact aattccagag atatctaagt ttctaaacaa catcattggc cctgaaggga
     5941 gtatgaagac gacctatctt aaatttgtta aaagtttgat tgatccgata gtcggcggag
     6001 agctatacaa tccgttgata gaccttaagc agatcgagca aatctttgcg cataattatt
     6061 ttgttgcttt cgacaacgtt tcagaagtta gaccggcgtt atctaacttt atctgtgcag
     6121 ccgttagcgg cacgtcggtc gattttcgaa aactattcac atcacaggaa attgtgagac
     6181 tggtgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnncaggaa attgtgagac tggtgatcaa gtgttgtgtt gctcttacaa gtattaatcg
     6421 agcatttatt gaacgagatg caggacggag atctctcaac gaagagttta ttgcaatcga
     6481 gcctggcaaa aattattacc aggacgaaac aataatcgca gcacgttttg agaaaatcaa
     6541 accgcagtta ctaggttaca tgttcagtac aatctccaag gcaattttaa tcaaagaacg
     6601 aattgcaggc aaatactcac tccaaagtat ggcagctagc caagaatggg gtgaagctat
     6661 atcgcaagca atgggctacg aagagggaga atttatagaa gcatatcaag aacttgataa
     6721 aattcaacgc gatcattatc tggactatga tccattgatc attgtatact cgaagctatg
     6781 ttataactta tttataaaaa accaagattt aagtcaggaa gaacgcaaga acttacctac
     6841 cgacgaatac gaacccctac gtcaaggttt caaagaatat cattacaaag agttgagttc
     6901 agaattagca cactatgccg agcctgaagg tatagagacg aagcctaaga ataaacaatg
     6961 gccacaaaat aacagggagt taaaggaacg gagtcttaag ctgtcaatgc aattactcaa
     7021 tgctcgttgt tattcggtcg acattaggca tgacgctcat aatgagactt tgtatgtact
     7081 aggcacaaaa gaaggcttgg aacgttatcg atctcttgac gatctgggga aggttgacaa
     7141 aaaccaagac attgttgtcc atgagtgcat caacattcta caacaatatg ataatatcga
     7201 catattgtta gaacacttac taaaattagc atctatccaa aaccctcaag ttggccttta
     7261 tatagataat aaatttaggc tacgtgatag tagaaaactc aacgcaatcc ttgaaaaatt
     7321 attggagaat ccttcggtag aaaagataca agactatcca attactttga gatggacacg
     7381 gggcaagcgt aaagaagaga atagtagtga aggtaatagt gggagtagcg atggtggtga
     7441 cgccactagc agtacggatg aaaacaaggt aacagaagac tcttctatac aaagtgttgc
     7501 aaaaacgtcc tcagttttca attttgagaa aaacgttttg gacaaatcac aacaagatct
     7561 ttcagccgcg aaccaagatc aattgtccga aaaaaaattt caaaatatga aaactaagcc
     7621 cgattttgca acacaatgta tagaagattc ttccattgaa tccgagaaat tagaaaactt
     7681 ggtgccggaa ctggccaact tcgctagctt cgattgtgaa tggcacagag aagaccttaa
     7741 agaaaacaag gaaaaagacc gtgaaaacga catctattgt ttctgtctaa ttgacaacac
     7801 tggccgtatt gaaaagtttc atgtcgatag gtttggtgga aaccgggttc tttttctgac
     7861 tacaattctt gaagctatgg aaaagtatga cttgctaact gggtattaca tttttggaga
     7921 ccgtgatatg gattcagacc taaaacatct tgaaaacaat tgtgctaaag tctgtttgga
     7981 ggagaggttt gctaaactaa aagaaaagtt aaaattcatg gatctttaca agatattttc
     8041 taatagggtt gtcgaagggt ttctggaagc tacctatgaa acagactacc gtgggtatgg
     8101 tttggatgag gttgtagc
//
LOCUS       DAOR01000121           61585 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_7330, whole genome shotgun sequence.
ACCESSION   DAOR01000121 DAOR01000000
VERSION     DAOR01000121.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 61585)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 61585)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..61585
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_7330"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    52156..52318
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    60170..60383
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        17938 a        12731 c        12424 g        18115 t
ORIGIN      
        1 atgcaaacgg tcgggagaat tcccaggtgg gtcattgaaa aagatccgtc gtgggagaaa
       61 gcagttcact ccaattatga catactggaa tggactaaaa aaggagctga cctgcgagca
      121 acttgtgatc cagcaagcat gttttgtgat gtcatgattg aggaaaggaa tcagcatctc
      181 gatctatcag atccgatttc gaaatttaac aataagagca gattgagcac attcgcctca
      241 gcagatatag caagatacct atcaagtaga tatctcaacc ctaggttcga tatcaatagg
      301 acggggcttt ttaaatttca caagctttag caagattatc gatggcataa aggcttaatt
      361 tatgcgtact atgcttcttt ggagcattgc agttattggt ctgttattat ttggagtatt
      421 tttaaacata ggggtttatg cccagtcgcc aaaaaaaagt gcgtacgtag atactgtgag
      481 atttattcaa tatctggatg ataacgttgc actgcaagag atcaaatccg gcaatttaga
      541 tacctatttt tttaggatcc cgctagaaac ggtttctgat gtcaaggctg atcctaactt
      601 gaaagtatat gacagaatcg cagggtcttt tgggctctta ctgaacccag ctccttcaaa
      661 agatgataat tcaataaacc ccttccaatt cagacaagtt cgttttgcga tgaattacct
      721 agtagatcgc gaatttgttg ttgacgaaat attaagaggc tatggtaccc ccttagctgc
      781 tccattcggc gtatattctc ctgaatatct taatgtcttg gatacgatag agtcatttgg
      841 tttcagatac aacccacagt tcgcacaaaa attgatttct gatacactta ctaaggcagg
      901 agcaacaaac cagggaggaa aatggttttt taaaggaagt cccattaagg ttaaaattct
      961 gatcaggagc gatgatccac agagactatc catgggcgag ctcgtggctt cagagctaca
     1021 gagcgcaggc tttaacgtcc aaacggattt tggagacttg aacaaggcaa atccgattgt
     1081 atatggctcg aatcctcaag actttctttg gcaggtttat acggaagcct atgctggtac
     1141 atctgcattc gtaaaatata acccggtggt tgcctctcag atgtacgcac catattatgg
     1201 gaacatgcct ggtgctcaaa atcctgcatt ctggaattat cgaaatgaga cactagacaa
     1261 attaacacaa agaatagaat tttttaattt cacatctgag ggacagagaa atgagctact
     1321 ccgaaatgcc accagggacg ggattgacga atctgtcaga atcttcatta cacaaaatac
     1381 ggagccgttt gttgcatctt ccacgataaa aggtttgatt aacgattttg gcgctggaat
     1441 cacaagtaaa ctttcactta tgaacgctag gactccgaaa agtagcaaca ctcttgacat
     1501 cggtgtcaaa cagatttacc aaggggcctg gaataatgta gagggttgta aagatgtgta
     1561 ttgcacacag atctattctg ctatatctga cagtgctacc tttagaaatc cctatacagg
     1621 cgaagtgatt ccaatgcgag agcattggat agacgtctct acgaagggtc ctacagggaa
     1681 gcttaaggtt ccctctgatg cccagatttg ggatccttat tcgcagcatt ggaaatctgt
     1741 gggccaaaac gcgtcatcaa tgagtaaagt cacctataaa attctctatt cgaattggca
     1801 caatgggatt tcgatggaca aatcggattt gctttatacg caatacttcc tctttcaatg
     1861 gggaaccaat acaggaaaag gcgatcttac gattgatcca gaattcacat ctcaagcgca
     1921 ggcagcactt ccactgatca aaggcttgag attcattgca aatgacgaaa tggagtctta
     1981 tattaacatg tggcactatg acaaaaagga aatcgccgac gcagcaacaa tttggccggc
     2041 tgagccatgg gaaataactg cagcaaccga gagacttgta acttcaggta agcttgctta
     2101 ttcgagtgga gaatctacag cgaaaaatgt tgattggcta tcgttgctga ttccggcaca
     2161 cgctgaaatg ataaaagaag aactccagaa aatgaaggat gaaggttatg ttccggctgc
     2221 tcttaaggga attgtaagcg tgggcaatgc aacgaagaga tatgatgcca gcataaactg
     2281 gatcacaaat cacaagaatg cagttatcgg aaatggcccg ttctacctag atagctataa
     2341 tcctgcaggt ggaataatta tcataaaggc atttagagat ccatcctatc ccttttccca
     2401 agggtattgg tcttcgtttg aaaatccgaa acttgcaaca cttcaaagta tcaacgctcc
     2461 acagttcata tcgattgggc aacaaccagt tgttgtcagc ctaactgtga atgtaggcgg
     2521 aaagcctagc aacgatacaa tcgttaacta cttcatatcg aataaagatg acaaggtagt
     2581 tgttcaagga gtagctaagc ctgcctctgc ggataacaaa ttaggtactt acaatatcat
     2641 attacgggaa aacgatactt caaaactgtc tgttggccct aacactttga aaatatttgc
     2701 aaatagcaag gaggcgttta agccggatat ttattctaca gtattaatag agaatttctc
     2761 ccgtttcaaa ggatgatgac acttgactag gttccatttt ttcttaatta gaagatcaat
     2821 aaatatgctg cttgtggtct tttcgactat ggtgctcacc cttgctcttc tcggctctac
     2881 aatggataag atactaatag atgcagtcag atttgatgaa gtgaacagtg taaaccaagg
     2941 aaaatttcag tttcaaagtc cagaccagcg tcaaaagtat attgatacac aaaccctgct
     3001 tcatatcaag accctgggct tggatgaacc ttggtattct ccaaagcgct tcgccaatac
     3061 actattagaa ctgctgactt tggatttagg aaaatcgcat ttttttacta gcgattccgg
     3121 ttcatctagc gtcagggaca taataataga aaaactgccg aagactgttt tattattcac
     3181 atcttcaact ataattgtgt cattgattgg tctttgtata ggtggctttg tggcagggaa
     3241 gtcgggttct atatgggata aaatgaattc attttttgca gtggtaagta gtagttttcc
     3301 ctcttggtgg gttgggatgt taatgatatt tgcgtttgca tttgtttacc agatctttcc
     3361 ggctcgctct acaccattaa ctcctccgtc agatccattg tatattccag acttgctcta
     3421 tcatatgaca ttacctctca ttactatcgt tttagtgagt tttagcgcat ggtcttacat
     3481 tgtcaaatat tttatagtag gcattttgca ggaagattat atcgcggcta aaatggcgat
     3541 cggacttcct aaaaaaagga tagtttattc gcatgccctg aaaaatgcag gcccgccaat
     3601 aataacagca attgcaataa gtttggcatc ctcatttgga ggagctataa taatcgaggc
     3661 ggtgtttgat tggcctggaa tggggaaatt atattatgat gcaatatcag taatggatgt
     3721 tcctattatc attgggctca cttacgtctt tacgctaatt ttcgttatca cgatttttct
     3781 cacagatatc atttacgcgt ttctagatcc acgagtgaaa gtaggatgac aattgaagta
     3841 atatcgcgca cgactatatt tcgaaagttt acgcactcta gaggcggaat agctggagca
     3901 attataatag tagtcttgat tttaatgact ttgtatgctg tattttatat accgctggat
     3961 tctttcaagc aatggaacaa tccggattat tggattgact accctaaagc tgctgcgccg
     4021 gcgtggacaa atattgggct gaaattgcct gaacatgtaa ttcttacttc tgaccaggcc
     4081 gtggtgtcga gtatgataca aaacggaata catatcgtga cacatactta tgctattaac
     4141 ttcaattacg attcttaccc aactgatttc atgctaccct atactgtgaa atacggacaa
     4201 ataccgccgg tcttgcagat agttataatt aggccggatg gtagagattt tcggatttat
     4261 agttctgcat taccaccttc acttatttcg aatggggctc aaaatgaatt cagcgcaaga
     4321 gtgttctctg ctgacagctt gataatcgag aaccttcgtc ttgataagag tcttttcaaa
     4381 tataaccaag acgaatcgct gcctcagata atgatatttt ctcaaatcga tcaagaaaag
     4441 gttttaaaag gaaaatatca ggtgaaggaa acattctatc tctttggtag ttcagactct
     4501 gtcgaaaagt ctggtctggt tctcggtgga agagtatatg gcttgatggg aaccgatgag
     4561 ctacgcagag atctcacaat cggcatccta tggggaaccc cggttgccct ctttatagga
     4621 ctaacggtct caatagcttc aatttccata gggttaattt acggggttgt agctggatat
     4681 aagggaaaaa gaaccgatga gggattgatg cgaataaatg acttatttta ttcgttacct
     4741 actctaccac ttttagttat tttatcagta ataatcggca gaagcatatt cttaataaca
     4801 ggtttccttg tgatttttgg gtgggtaggc actgcaaaaa tcattagaag ccttgcattg
     4861 cagttgaaaa atttacagta tgtagaagct gcgaaattaa tgggacagtc tgatataaag
     4921 attatattta aacatgttat accccaattg cttccactga cctttgctag cattgcaata
     4981 gcagtcccaa gtgctatatt gggagaggca gcgctaagtt tcttagggtt gggagatccg
     5041 tctattccca cgtggggaca aattcttcat gaagctaatt ctggacaagc tgcagctcgg
     5101 ggtctatggt ggtggataat tccgcctggt ttgatgattg cactcacagg acttgcgttt
     5161 gcgcttatgg gtaatgcagt tgaatctact gtaaatgtca agggcagata ctagcgattg
     5221 tagaacaaga tatagcactg ctcttgctgc tctctcgtca tatcacgtca acgaattcta
     5281 gatccatagc cacaccattt ctatcgaatg cgccgatggt attatattca tagggataat
     5341 ttatgataat cgagatgacg ccataataat tattcagatc ctcaagtgag ggtctattag
     5401 aaccgccggg atgggagtgc gctgtcccta cataagagag atcgaaaggc aagtcgtaag
     5461 tcggaaaacc ggaataatat ggtccatgtg tagaaaaggg cggaatgact aatccttcaa
     5521 taatgatttg ttgtttcttg ctcctgcctt tcaaaattaa aattccttcg tttggatgcc
     5581 atgacttgga atatgtgata atcccatcag caacctgttt ggtcatcgtg accttccgac
     5641 taaccgggtc aggctttttg cctttagata ggaacaataa tactgaatta gtttgcatct
     5701 tttattttaa gattggttgc ctagcatatc acaaagacta aaaaatggct tagccgagta
     5761 caccagtctg gtggtaagca atcctggata catgctttcc agcatatgca tgcatttggc
     5821 tacctttttt aatagcacga tacagaaaag tattatacga atgacaaatc aatgtgctct
     5881 tttgctgctg gaattgggtc tagccgcatt ggatgagaac aacaatttaa tcgtctcgag
     5941 aagattcaca aatcctgccc aatcgttccg ttcgataaag tcaggaaaga ttccgatgga
     6001 acttgaagag attattgagg tgctttccca ttttgattat atatctgtta ataattcgaa
     6061 tgtaaatgac gttctgagat ccgccggact taaaagtcac atgatgcctc tgcaggaaca
     6121 agatgaaatt cagaacaaaa agcaggagct gctaattagg tatggatttg cacgtgatga
     6181 aagagataca atacgtgaat taagaaattt tgcaattgaa atatcgtcat cgagagtaaa
     6241 agaagaatcg caaaaattag acctccatgt ggctcaggcg attaatgctc ttgacgaaat
     6301 tgaccaaatc cttaacacta taggggctag gatgcgggag tggtatggtt tacacttccc
     6361 tgaactggac aatttaatta cgagcataac tgcctatgcc gaaattgtaa aaagggctgg
     6421 gtctcgagaa aacgtgtctg aggaaatttt gaacgccgtc ggtattgagg atagaaaagc
     6481 ggatataata atttctgctt gtaaaaggag cagaggaggc gatataactg cagaaaatat
     6541 cgaaattgta aaaaagcttg cagagcaaat aatcacgcaa tcagcgctgc gtaagacatt
     6601 agcagaacac gtagaattcc tgatggaaag cgtggctcca aacatcaaag agttattgac
     6661 ggcaccggtt ggggcaagag ttattgctaa agcaggaagc ttaaacaaat tggctatcct
     6721 tcctgctagt accattcagg tattgggcgc agagaaggcg ttattcagat cgcttcggac
     6781 aggagcaaat ccgccaaaac acgggctcct ctttcaacat tcacttatac attctgcacc
     6841 taaatggcag cgtgggaaaa tcgcaagggc gatggcctca aagttagcca ttgctgcaag
     6901 aatcgatctt tttcgccctg gtgtgaagga ttcatctata tcgaatcgtc tgaatctccg
     6961 tattgctgag attcaagaga aatataaaga acctagcgtg caaaagaaaa gaaacgatgg
     7021 atacaaagcc catggtcggc ccccttatcc agaggattct aaacgtttta aaaatagaaa
     7081 ccctcgaagg aaaaagttct cgaagcgtcg attctagggg gaaaagggaa aacgtctcaa
     7141 catggttata atggagtttg ccgaagaaat ttcgggaatt aagtagattg agggctagaa
     7201 ggtttgatcg aatccaatct tgactcggtt gcttggataa ctatccacgg cgaaaagcaa
     7261 attgcgactt tgaatttagt caaaggtatt gacgtttacg gagagaagct ggtcaaatac
     7321 gatggagagg aatatcgagt ctgggatcca ttccgaagta aacttgcagg agctttacaa
     7381 aatggactca ctgaccttcc ggtcatgaat aaaagcaaag tattgtacat aggcgcctcg
     7441 accggcacga ctgtaagcca tatctccgat attattggaa ttaatggtat aatctttgca
     7501 gtagaatcct cttcaagagt tgggagagag cttttagaaa atctagcttc taagcgagag
     7561 aatattgtgc ccatactcga ggatgcccgg aaacctagat cttatttttc tgttttcggg
     7621 acagtcgatg taatgtattg tgatatcgcc cagcctgacc aaactgaaat tgccatggcg
     7681 aattgtgttg tttacctcaa acctaatggt gtattgctat tggtggtcaa aactaggagc
     7741 atcaatgtac ttctgaatcc caagactgtt attgctcaag aggcaacgaa actgcaaaat
     7801 aacaggttta gtattaagca aattatagat ttaaagcctt ttgataaaga ccacgcatta
     7861 atacgcgcaa tttatcagcc tcaataggca gatgcgctct cgctttcgag cataattctt
     7921 aaaggtctcc acgattttct ggcaaaatgg ggcgcagact tgtatcgggt aacccatttt
     7981 ttgatgaaaa acatggtttt cttgtctgag gggtaaccgc ttcctatgtc atgataaatc
     8041 ttacgaattc gttcaacttc attatcacga atgattttgg cgacgataga tgcggctgaa
     8101 acaactacat ttaaacaatc tgcatgatgc aaacagtaaa tccttggttt tggagtccac
     8161 aaatactttt ttattgaatt tttgtagcgt tttagattga tatcacacgc atcgacgtag
     8221 attctgtcag catggatatt gttgataacc tcagccattc ctatcgcttc aagctcgttt
     8281 agaccgttaa aaaagacata gttgtcgact tcatgacagt cgaatttgga tatgcaaaga
     8341 gagtttgcca atcctacgat atttccaaag aaattttcgc gggccatggg cgtcagcgtc
     8401 tttgagtctc ttatacctaa cttgtgcaat cgggatatgc caatttttcc gatgcttata
     8461 cctgcaacga ccaaaggtcc aataacagat ccacgtcctg cctcatctac tccacctata
     8521 agtgagtctt caatggtttt ttgcaagaac gatttatcct tgtagcgggt tgatttcata
     8581 tcaaggaggt tattattagt tatgatggcg aaacgtgtct attactgata aataacggaa
     8641 cgataaatac aaggcccaat actgttatga gctcctctga gtttgtagaa accatggaag
     8701 gcaagacgag actattggtc ccggcctcat cactttcagg taaagttcca ccaaaagttc
     8761 cagctttctt taacccgtca gccagattga atcgggatat ctcagtactt gtatacaaga
     8821 catttgtacc ggagataaaa aaaaatccta aaacttttgg tgatccattt ggaggaatcg
     8881 gagcaagagc attaagagtc gcagttgaag tcccagagct tgaacaaatt tatatcaacg
     8941 atataaacag cgttgcaata gacgcagcaa aaaaagctgc tcaaatcaat ctggtaacac
     9001 aaaagtgtac ttttacaaca caggatgtgg tcggatttct gacgagccat gattcaaaaa
     9061 attacgagag gttttcgata gtagacctag atccgttcgg cagtccatca gcctatatag
     9121 attgccttct aagatcggta aacaagggag ggatggtctc catcactgct acagatacgg
     9181 ctgttctgtg cggcgtacac ccacaagtct gtatgcgcaa atactacgga aagccactta
     9241 acaaccatta tgcacgtgaa actgcgcttc ggctaataat atctttaacc gcactaatcg
     9301 cggcaagact cgatcttgtt gtccatccga tattcgtcca tgccaatctt cattatttga
     9361 gagtatacct tactgtatca gttagtcgcc ttgaggccaa ctcggtcttt aaacgcattg
     9421 gctacttgcg agactgtaca aagtgcggaa ataggaatgc agttacagaa tataatagag
     9481 gagaaccgtg tgaactatgt ggaagcacct acagttttgc tggctatttg tggattacga
     9541 gattgttcga caaggatttt gttaaaaaaa tgagttattt gttggacaca gatgacgaag
     9601 tatttgctcc aatgcaatac ctgaagaaaa ttctggttac atgcactgaa gagcttgatg
     9661 atattccatt ttatttcctc tccgatgaaa tcgcatccag gttaatgaca aatcctaaac
     9721 cattgcaaaa aattattgag cagctacgct cgattggtta cagagcctct agaacaagtt
     9781 tagatcctaa cggttttaag accgatgcac ggatagatca gatactggat cttcttaaat
     9841 aaatcaatca cagatattga gttaatcttc ttattgctac ttcatttatt accatgctgt
     9901 gtcttgactc agcaaaaatc catgtgctct actaattatt ccataacgac gacttctctg
     9961 tggagatatt gcctaaaagg tctgttgtgt ttttcatacc acgtcgatct acacgtaatg
    10021 caccttgaac catttgtaat tgattgaaca acgatttagg ttgtactgcg attgggataa
    10081 cgtgagtaac ttcttggcag tatagattaa atcctacaga ataccccatc atatatcctc
    10141 aggaaggaga ctttgtaaat gactaaactt gagcttagga atgttaccaa agggttcgag
    10201 gcaaacaatg gagcgagttc tgtgaccgca ctagaaaata taaatctaaa tattgaagag
    10261 ggacagttcg tatgttttgt cggcccatca gggtgcggca agaccacgtt gctgaacatt
    10321 ttagcaggac tcgacaagcc aacttctggc gaagtaattt tgaacggtca accggttcga
    10381 gaaacgggac cagatagaat tatggtattc caagagaatg cgttattccc atggctcaga
    10441 gtgatagaca acgtggaatt tggtttgaaa atggctggca ttgaaaagga aaaaagacac
    10501 gagatagcga tgcattttct tgacatgatg cagttgaaaa aatttgccga agcatttacc
    10561 taccaactat caggcggtat gaagcaacgt gttgcaatcg ctcgcgcact cgtcacggac
    10621 ccagaagtgt tactaatgga tgagcctttt gcagctcttg attcacaaac tagagacctg
    10681 ctcatggtag aattacaact gatctgggcg cgaacaagga aaacgattgt ttttgttaca
    10741 cacaacatag ccgagtcagt ttgccttggg gacaaagtcg tgatatttac tagtaggccg
    10801 ggaaaagtca aaaaggaaat agctgttgaa tacaggcggc cgaggttaag cgaagataac
    10861 aatttgtaca gttatcagag aaagattctt gatgaacttg ctacagaagt aattacagct
    10921 cgtaaaaatg aggatgcaat ctgatttagt caggcctatt cccgtaatct ttcaagtagt
    10981 tctacacctg acctaaaact attgggtgaa gtaagtagaa cttcatttgc gagatctccg
    11041 gcctccttta taaagtcaac tgcattattt tgatattctt tccaatccaa cccgatcgtt
    11101 tctgcagaca tctgattatt attagacggc accatgatac acgccactac tgggatgttg
    11161 tgagatcttg caacatcgac gatctcacct agctcagcaa gagatgcgat cgattgagtt
    11221 acgaacgcac ttggtttagc attaattttt ttttgaatag atgatggact cttaatttta
    11281 gcaggaatcg tcaaataaaa atcgatcaca gagttataat tttcgtaatt tagcatcttc
    11341 aacacactac tcggctttaa gcccaagtct atagatccgc ctacaggatc gtctcccatc
    11401 agaaccagga ctcccttaac tcctatcagg attgcatcag atacaaattg acagattgag
    11461 gtgtaattcc tatctcgggc tctaacgcta caactaagat ttagcgggtt gccagttctt
    11521 tttatcaaat tggccgcagt aacactagat atgcgaggaa tcccgaggac agagtcagtc
    11581 aagtgaattc catttaccag atcaaccaac ttagatgcac gtttagtaaa tttttgtatc
    11641 tcttggttga gtaggaccaa atcgaaacgt tgatccttta atatttttgg cgggtttaat
    11701 tcgaaagtaa tcttcattat atctgctaat catcgtaaac ggtaacctac tattaaactc
    11761 ggacgcaatt ctacaatttc gacattcgtc gcgtgattac tactactgtt gacctactgt
    11821 tgacctactg tcgtcggtcc cccagcatct ctcttaacgg aagggctttt ttttcagata
    11881 taacgtttaa ttttgatatc tctattacag ggtaatgttg aacaaagagg atctcaattt
    11941 cagtctccga cagatcttat cgcaatggcc ctcgcagttg acttcgtctc cgtcccttcg
    12001 aatggctgct gttcttgtaa taatccatta cacccaatcg tcaccgcgaa ttctgttgac
    12061 gaagagaaac tcgacactca agacccacaa aggagaaatt gcatttccag gaggtgcatt
    12121 ttcgaaacaa gataagtcat tatgcgatac agctgcaaga gagaccaggg aagagatcgg
    12181 tctgatcata gaacagaaag atattttggg aagtttgcaa tcggttcgaa cattgacttc
    12241 aaaatttttt attattcctt ttgttactat acaggagagg attctagaac ctcaaattct
    12301 gatcaacgaa gtgcaggagg ttattgatgc tcctcttttt gatctactgg atacggaaaa
    12361 aattgatctg gagcatcaag ctatgtctac aaaaagcctt tatgaattca cgtacggaaa
    12421 cgaggtaatt tggggagcta ctgccagaat cttaaaagag ctgcatgatt gcctttatgc
    12481 aaagcgtgat ttttgttgaa aaggcctttg tcgtctgtac acatcatcca ttccttgact
    12541 agttaaataa gcaatacgta caaaaaagtg ccttgcatac tgtggaggat aatgtttgtt
    12601 tagtaatctt ttgagctctt caagttttga tgttctttct cccaccttat caaaatggac
    12661 agaggtctga tcatgctcgt taattctgtc gttcaatgga tgttgtgctt gttgtgattc
    12721 gggtgagtga tatggtggtg gcggatatga cgacgacgac aacggcattc aagttttatt
    12781 actcatattg gtttcgagtt gagtaggtct attgggcgaa ggttgttgaa gtagacgtat
    12841 tacctaggaa ttcatgttgg gtcaaattcg gacgtctgaa ctgattccct atagtgtctg
    12901 aaatggccaa agctagtact attgttatta tcagaggaag tttgccctac ctgattgttc
    12961 ctgtaccatg aaggatcgct agctaggtat tgttccgata ctctgcttac tatatagtca
    13021 aggagacgga caacctccga tgtactgttc tatgcttgtg cctcatcccg ctgtatttgc
    13081 gagtaaaagt ttgcgaacat gcagtacaga cgtaattaat ttgacgcatt ctatcgctat
    13141 aaataataat gactccagtt attaaagcat agcgtcacgc agtgtcgcat tgtcacataa
    13201 gagaagtaca gaaaattctt ccacattgtt gagtgtccag gatttcatac gttagtttgc
    13261 cttcaagacc tcaagactag agagaacaaa accagagcag agtttgaact gtaataatgg
    13321 cttaaattag atttggtaga ctacgaaaaa aagcaatatt tgcaccatca aatcagacgg
    13381 agaatttacg aatgtcttgt tccatatttg agactttgga tttcgctaat caagctttca
    13441 tagagcttcg acaatctcta aatctagagc tttcccattt ctatcaaatg ctccaattgt
    13501 actatattca tagggatgac cgagccgtct ctatgcattt tgaagagtcg atgttaaata
    13561 tacaggtttg accaaatccg ctacgtcaac ccaacatttt gcaatcttat ccatggaatg
    13621 aatagaattc agaactgcaa tagttaattc gtaatcggtc aattctattt ttgcgagcaa
    13681 ctcctttatc gagtccatga taagtctaaa ttcgttgtac attttgatca tcccaatgga
    13741 ttcagatcta ttctttttgg tgaaagcagc caccgatttt tcctgcattt tctctacgta
    13801 actacctgct tcgctaatct catcgacaat tttagtccta gaaatagatg ggactgtcga
    13861 tgcaaattcc ccaatatagt caccggcact ttcgagatga ttggctgcta ttctataatc
    13921 caagatgtcg atattgctca agttaagctt ccgtgccaat ttctggtcca ccattgcgct
    13981 gcgaattaac ctgacaagca gaaagtattg cctgtcaact tcatcgtccc gactaaaaat
    14041 caaattctta ataccttctg atttcagcct taggccatcc aaggcatctc ggtacatccc
    14101 tcctacaatt gagttcattc tccggaggat tttctctgca tctagagtat tagcatcaag
    14161 taaaaactgt atggcaatgt ttgaattatc ctcgtccact atctccaaac ccacgagttt
    14221 tctcatcgtt agctttatgt gctctcgatg ttcgaagagg atctggcgtt tgcctttgat
    14281 acgtatttca ctgtagccca gaaggtaggc accatatatt tgattgatca ggctgtcaac
    14341 ggaggattcg ctgtacaaga tagtaacatc ttttatttgg ctgtcacttg cttccgacgg
    14401 aaataatgag attgagttgt cttcattagt ttcaatgatc actgtatttc ctttattcag
    14461 acggttagtt ctaacccagt cactgggcaa agaaataagc atactactac ctattttctg
    14521 taacctcctt gcgtacttca aatcaatatt ttatactaat ttatactaat taaacattat
    14581 ttttatattt tatgtactct ataaactaaa tgtgtcaaca gtcgttacac agcccgttgc
    14641 tatagccaag gcctttagcc caggtcatat tactggtctc gtaaagagag cttccgttcc
    14701 atgtcggcac tttctgtatc agggatctga gggtgctggg ttttcgatcg atcgaggagt
    14761 tacaacgact gttagcctgt tcgacgatcg caattcccgt tatcaaattt caattaacgg
    14821 gagaactgtt gtggatgcag acgtatcaca attagttttg caacaatatc tgaaatttat
    14881 tgaacgacca tattatatta aaattgaaca tgatatcgag atcccaattg gctttggact
    14941 aggttcaagt ggggcagcag ctctcagtct ttcatatgct cttaaccagt ctttgaaggt
    15001 agggctaagt gcagaacaag cagcgcaaat tgctcactac gctgagattg tatgcagaac
    15061 aggattaggg actgtaatag cagaatatac gggagggttt gagacgcgga cgagtgctgg
    15121 agcaccaggg attggcatta tcaggaaaat tgaactcaag ggccataggg caatcatatt
    15181 ctgtatggct ccagtttcta ctaaatcgat cctatccgac aattttgatt ttagatctgg
    15241 ttggcacgac aacacatggt taaacaatct aaaggctgcg gtggacataa gagaatttgt
    15301 aaacatggcc tatgagtttg tatcaaatgt gggtttgaca gagggtcgat gcaaggatcc
    15361 aattagcagc ttgaaatcac acggttttga ctctagtgtg gcgttattcg gtgacacaat
    15421 tttcacaata gttccagaag accgggtccg tgaggcggaa cagtgccttg ttggccataa
    15481 cggtcaatta ttcacatgtg gcatagacaa tatcggtgca agagtgctgt agagtattaa
    15541 tgagtggcaa aattccctcg agccatccca gattgaaatc acttctgatt cgagagaagc
    15601 ttgtgaatgg ttttctgcgg ggattggtcg cggcagaggg tttgatagct catgggaggg
    15661 gagaggcttt cgattacctt ctaggcgagc ggactaccga atctgcccat gttgcaataa
    15721 ctactgcatc taccgcgcta ctactcgcaa ggcatccggt gatttctgtt aatgggaatg
    15781 ttgctgcaat ttgtgcaaaa gaaattgtcg agctaagcaa agttgcgaat gcactgttag
    15841 aggtaaatct tttctacaga gacgaacggc gagagcgatc tatcgaaaaa gaactaataa
    15901 aaaacggcgc aagaaaggtc tacggggtcg gttcacgctc ttcagcaaga atatctgaac
    15961 tgcaaagtga gcgacgaaga gtagaccccc atggtattta cgtggctgac gttgttattg
    16021 tccctctaga agatggagac agagcggagg cccttgtcaa gatgggcaaa aagattatcg
    16081 cgatagatct gaacccgctc tctagaaccg cgagagtggc tcaaattaca atagtagata
    16141 atattacccg agcattgcca acaatggttt ccatcgtaag aaaactcaaa tgtcccaaag
    16201 acgatgcgat actcaccaaa attatcatgg gatttaacaa taaagagaac cttgcacatt
    16261 cattaaaaat tattcaaggg ggtaaaaatg cagcggcatt atcaaaaaaa atgcaataac
    16321 accaacccac ttgtcagaag aaacaacgtc aatgatattc ttatgctaaa gaaaaaagga
    16381 gagaaaatcg cagcgctgac tgcatatgac tactctacct cgttgatctg tgacaaggca
    16441 ggtgtcgata ttattcttgt aggcgatagt gcgggtatgg ttatgttagg gtataaaagt
    16501 acaatgcccg ttggaatgaa ggatatgctt gtcttttgca agggagtagc catgggaaca
    16561 aaacgggcaa tggtggtggc cgacctgcct ttcggttctt atcaacatag catgggtgct
    16621 gctataaaaa atgctataag atttataaaa gttggctgcg atgcggtaaa acttgagggg
    16681 ggaaccgaag tagtggatac gataaaggca ttagtttcca tgggcattcc ggtcatgggg
    16741 catatcgggc taaagcctca gacttcttct ttatgggagg gctacaaagt gcaaggtaaa
    16801 acaaaaaatt cgtcacaaaa actaattacg gaggccagag ccctagaaca agcaggcgta
    16861 tttagcatag ttctcgaaat ggtaacaagc gaagttgcta gcatcatctc acaagacctt
    16921 tctgtggcag taattggaat aggttctggt tctggttgcg acggtcaagt cctagtatta
    16981 cacgacatgc ttggcatata tgaagatata aagccaaaat tcgtgaaacg atatgccgac
    17041 ttatctcatt ccatttttca agccgtctca ctttataggt ctgatgtcaa aagtgagaaa
    17101 tttccagaag aagagaacgt ttttcatatg aattctaatg aatacagaga attgcaaaaa
    17161 aatactacaa aaaaaaatag ttgagtgact gaatgcattt agcagataaa agggggagac
    17221 atccatctaa agacatagta gctacgcaag gaaaagaaat tttaggaaaa aaaatagtgc
    17281 tttgtatcac aggcagtgtc gcggcctaca aggcgatcga cttagcacgt ctactgatgc
    17341 gacacggggc agatgtctat gctgtaatgt ccgaatcatc tacttcgacg ctgattaaag
    17401 ccgatatcat gaaatgggct acaggtaatg aagttgtgac aaaattaact ggaaaattag
    17461 agcacatagc gttagctgat actggtatgt cagatctcat tcttgtttac ccatgcactg
    17521 ctaatacgat aggtaagatt gcgaatggga tagacgacac aactgtttct tctgttttga
    17581 ctgttgctct tggttcgaga atccccatca ttgttgcacc cgctatgcat gagtcgatgt
    17641 atacaaacga cataatcaga caaaatgtcg atcggttaaa ggatataggc gtgaaatttt
    17701 tagaaccttc tgttgaagag ggcaaagcca agatagtgga accggaattg gttttgcaat
    17761 cggttatgag agaacttaac gggatgaata catcgttctc gttggaaggg aagaacattc
    17821 tagtgacggc tgggagcaca gtcgagcaca ttgaccctat tcgggttatc acgaatctaa
    17881 gctcaggtaa aatgggaatt gcgattgcag aaggagcact gcttgcaggt gcaaaagtta
    17941 cgcttgtata cggtcatgga tcggatggca cacgcgtccc aataaaaata agaaatgtga
    18001 tcaaagtaaa cacaagcaga gaaatggaga taactatctt gtcggaatta tcttcaaaga
    18061 gatatgatat tgttattttc tgtgctgcaa taacggactt tgcccctcaa cagacatcgc
    18121 ctaaaaaaat agagactaaa aaaggcaaat tcgtattacc actgttacct acgacaaaaa
    18181 taatagatcg agttaagcct atgagcagaa ataataaact ctttttagtc gcgttcaaag
    18241 cggaacacga tgtctcagat tcgtacttaa ttaataaggc atttcagaaa ttgcacgagt
    18301 gcgatggaga cttagtaata gcgaatgatt taggtaggaa aggttgtgga accggttctg
    18361 acgagaacga ggttttcata atagatagac agaagaaagt tattcatatg ccgctgcaag
    18421 ataaatatca ggtagccaga aaaatacttg atacaattgc aaatctatgc caagtacaaa
    18481 aagattccaa cagataataa tctctgtact gtacttctga tttatttctg ttcaaacccc
    18541 gtgccgggtt gatattcgat ccgaatgtct atcaaatatc aagttcataa ttttcatcgt
    18601 gatatagcat gtgagagtat gtgtacgttt tgaccctcga aggaatttaa tatcgcattc
    18661 atattgtaag tagaaatggt attagaatgc ctcattcagg cccatgtggc attcgggtcc
    18721 gttagtatcc agattggatc tgacgacctg cgagaagcat cagctaaata taacaaaatt
    18781 cagatgtaaa ccgtctgctt cagccgagta cgagaagatc tttcgtgaaa gcgtttaagt
    18841 tcttcgcctg cccattacct acaatgacgc tgtcctcaat ccttacgcca aacttaccat
    18901 ttagatatat cccgggttca atagttatgg ccatgtttct ctttaaaacc tgttggtttg
    18961 gagtcctcaa ccaagggggc tcatggacat caagacctat tccatgtccc gttgagtgga
    19021 taaactgatt tccgtatccc ccataactga tcaatgctct gcactcttta tctactcgct
    19081 cgcaaacgac gccttcctca gcgcagttaa tccctagttg ttgagactgt ttgactagtt
    19141 catatgcttc tttggtttcc gctgccgttg tacctaatgc aaaagttcta gtcgcgtctg
    19201 ctatatacga ggcatatcgg agagtcaagt ctactactac catgtcgcca tcgacgaatt
    19261 tacgatcgga aacctgtgca tggggcaggg ccccatttgg accgctcgcg attatcaaag
    19321 gattcaacgt tgatttcaga catgcagaat tggcacccat ttgcatggct tcaaatatta
    19381 tcttagcctg caaagcacgc tctgatagcc caattttgat ttcgtgtgtg catatttcat
    19441 ataaatcatc gaggactttt gccgccttag atattgcata aatttcgttc tcctccttga
    19501 tcattcgtcc ttcaagtaat gcatctgcat taacaataaa gtgtttttta gaggccttcg
    19561 tttggattgc ttcaacggta gggtagtctg catagtctgc acataccact ttgtctttaa
    19621 ccgcattgat aaacgtgtca attaattgaa atcctctctc cgagataatc acttcgcagt
    19681 ctttggaatc ctgttgtgct cttgaagcct ccaatttagg aactaccaac gaagttctat
    19741 cattgctgca aatagcaatc gcctcgcccc aaaaattggt aagatagaac acattttcgg
    19801 gctgaaatgc agctacagtg ttgaacccct ttgctgatgc aatttgcatg attctcgagc
    19861 gacgattttt cattctcaca tattacgatc ggacaggctt atgatcgtat tgcactctta
    19921 agagcagatc ggcgtaatat ctgctacgag tcaatactca aatggaccaa caaacattat
    19981 ggctaacaat gttgcgtaac gtcggtgcct ttagcacaca gacagtcgat gtaattataa
    20041 atatcggaag gtaattcggt catttatcca acatggaata cctcgttatg cttcctggtc
    20101 caaccaacgt accagatcga gtgatgaacg ctatgctaac acatattatc aatcatagaa
    20161 gtgatgactt tcgtcgtctt tacaagacca ttgttgaaaa gacacaaagg gtattccaaa
    20221 cacagaatga tatcgttttg ttgaccacat cagggacagg cgcagtagaa gcatctgtta
    20281 tcaatatgat cagaaaaaac gacaaagcga tcattcctgt taacggcgaa ttcggaacac
    20341 gtctagcaga cctgatagac agctggggag gtcatgcgat aagagtcacc gcaccgtttg
    20401 gtcagaatcc accgtattcg gaaatcgaag atgcttttga ccgcaacaag gatattaaag
    20461 ccatatatgc agtttacaac gagacgtcta ccggaacaac cttgcgatat atggacaagc
    20521 tgagtcaatt gtgttccgaa aggggtgcct ttttcattgc agacgcagtt tcaattctcg
    20581 gtggcgacga gttaccggtt gacaaatgga atgtcgatat ttgtgtaaca gcttcccata
    20641 aggcgttggc agcgccacca ggagtatctc caatgtcgat tagcgcgagg gcgaaaaaac
    20701 atatgcaagc gaaccctcct ccaacacaat accttaactt aaaaagatac ttcaaatatt
    20761 acgaagaaaa ttttgaaacc ccattcactc ctgccttgcc actgttcaat gctttcagtg
    20821 tagcactcga catggttctt gaggaagggt tggacaaaag aattgcgcgt cacaaagcct
    20881 gcgcagatgc attttacgca ggtctggatg cgatgggcct atcacctttt gcgaggcaag
    20941 atgctaggag caatgtagta attgctatca actatatacc gggaatggat gacaagaaat
    21001 ttagaggatt actttcacaa caatttagga ttcttgttgc cggcggcttt ggcgacctca
    21061 agggtaaagt gttccgaatc ggatcaatgg gtgaggttag taagtatcat gtgatgagaa
    21121 ccttgtcctc tatcgcatca gtcatgaaca ttttgggatt aaaggtgagt ccagatgcag
    21181 gtactattgc actaaaaaaa cttgaaagtg ttatctaacc tttttttggt accgtcgttc
    21241 ccatgaagat taatataagg taaatcggat gtcaaatttc atgactttag agaagggctc
    21301 ccttatattg atagattata cagctaacat taaagatacc aacaaaattt ttgaaaccac
    21361 cagtgaggaa gacgccaaaa attcagatct gtacgacccg acgcgcaaat atggaccaag
    21421 acttgtctct gtcggtgaag gttgggtttt aaagggtcta gacgaggctt tggcagaagc
    21481 aaacagtggc gataaactcg atgtagatgt ctctccagac aaaggatttg gagaaaggat
    21541 tccgaataag gtgagaatgg tcgctcagag gaaactagga gacaaagcag acgaagtcag
    21601 aataggtgat gaggtggaaa ttgatgaacg cacaggtatt gtcagattcg taggatcagg
    21661 aagagttcag gtcgatttca atcacagatt agcaggtcgg accttaacat ataaggtgag
    21721 cataataaag aaactagaaa gtgataatga caaagtgctc tcattgatga aaagaagact
    21781 gccgcttgac gatgaaaaaa taaaattcat tcttgaagga tcaaacttaa ctatccagtt
    21841 cccagaagaa acctttctga ccgaaggatt acaagttatc aagcgtggga tagccagtga
    21901 catcttcaaa tatgttggta gcatcacaaa cgttaaattc gtagaaactt acaaggcgac
    21961 caattcgaca gagtctaacg aggagtcaga agacgacgtt cactctagcc aagaagttgg
    22021 ggcaatggaa gtgtctagga gcgagtaaga aaagtgaagg ggtgtgtgtg cataagggct
    22081 ttttccacat cttaaataac ctttaccgac ctagcaagtt caaatgccga acttttattt
    22141 aatttggctt tactgagaat tgtatacctg tcaggtctca acgattgagc ggctagcaag
    22201 gagagaacta gttgctcttc attcaatctt atttgttttg cttttgtcgg tgcaccgact
    22261 cgttccaacg tttctacaat aatctcccaa tcaatgttat gaagtttagc catcatgata
    22321 gtacccaggc cgactcgttc cccgtgaagc ccataattct gaccaacgat gtattctacg
    22381 gcatgactga aaaggtgttc cgcacctgag caaggcctac tgcttcctgc tatacaagct
    22441 gccaccccag cgctgatgag agcctccaca acaatacgaa ttccggatct gcttctgacc
    22501 cgcaaccttc tcgattcatt caatataatt tttgcactca tatatgcaag gttagctgcg
    22561 taacttccaa aatattcatt attctcatct cgggcaagtt cccaatcctt gatcgccgta
    22621 atcttcgcga caaggtcccc acacccgctt gccattaaac ggataggtgc ttcggcaagc
    22681 aatgtagtgt cggctaaaac tccaagtggg gtgtttgctt ttactgaata cggttttttg
    22741 gagcccctta tcgaaacaaa tgggctagaa atcccatcat gtgaagctga tgtaggtaca
    22801 ctgatgaatg gtttgtttag ctgaaatgcg gtcattttag ctacgtcaac ggatcgtcct
    22861 cctccaaatc ctataatgat atctggaatg gcctttgcaa gtgacttttt taatttatta
    22921 acagaagcga tggacgaatc aactacaaca taccaggtgt catctattga cattgattga
    22981 aacgacgacg taacgtctgc acttgctctc ctttttacgg tgttccctgt tacaaaggcg
    23041 actttggatg ccgaataacc gatatctctg acgaacgcac caagactaga aatgacacca
    23101 tcgcctatca atatttttct gggtagttcc attaagtgag taggcgctga tgccatggca
    23161 accacgttaa gtacgagata tatttaatct tgtttgcatg aataagacgt ttcctctacg
    23221 aatttgaggc agtaccgtct ctaaacatat aatcgtacag cccaaaagtg cggcttcaga
    23281 aaatgtacaa gattttgcaa aaattaagtg taactcagat caggacgtga ttatgaattg
    23341 ctcaagttat cagtttctgc gacatatcag aacctctaca gcttttcttc cgcatcttcc
    23401 tcatgtgtgg ttacatcctt attttcttct tggtcttctg ttgtggagga agttcctgaa
    23461 gcagtgtcct cagcagaagg ctcgcttgct atctgcgatg ttgtatctga agttgcagca
    23521 atctctgctg tcgcatatcc tctgcccact tttgttatcc ttatctttgc agtgtcgcct
    23581 acgtttgtgc ctgcaacaaa gataaccagc ccttccacct tcgccacacc tgcgtcgcct
    23641 cttttactca tcgattcaat cttcacctca aactcttcac caacttttac tggggctggt
    23701 ctgaatggtc tggtccctcc aaaccctccg ccacctcttc ccccgtagcc tccgctgtaa
    23761 cctctatcgc ccctgtaacc tccaccgctg ctaccgtagc tacctcgttg ttcactagta
    23821 cgtgagtctc tgtcttccaa gctatctcag tgaggtatta taaatgcact tagtatttca
    23881 actttagtgt tataattggt agttgagcaa ttttatttct attatggagc actttaagga
    23941 ttatctgtcg aaacactctt taaatgggat tgttggatga tggaatgtaa tagtgctgca
    24001 ttagtttcga taaatgtttt cgaaaaaata taaagatagt gattaaaatc aaatgcaacg
    24061 taaacagccc gaccaagaat tcgcaggtca taatattatg gctgctatac tccaaaattt
    24121 gccaagcgag gctgaactca caaaaattga gtacgaggga ccacgtatag ctctatattc
    24181 aaagaatcca ggctatttgt tgaaaaatgc tcaagtagtt tctaatatgg tcaacaccat
    24241 aaaaaaaaga atagttattc gtatcgacga atccttacga atacctgagc acgaggcatt
    24301 tgaactaata aggaagacac tcccgactga gattggtgta gccaatatct tctttgaccc
    24361 agctttggga gaggccgtga tatttgcaga cagaccttcg ttaatatcca aaactaatga
    24421 tgatattgtc aatgttgagc tagcagaaaa aacaggttgg aaggtagaaa tcagaaaggc
    24481 gccgcataca atctcaacaa tagaggaaat taataagata ctgcaagaca ccgttactca
    24541 acgagttcac ttctacaaag aagtaggtga gaaaattttt aggcttaggc tagacgaaat
    24601 agtggaagca agtttaatca ctttaggggg atttgccgag gtcgggaggt ctgcaatcat
    24661 tctgtcgact catgaaagca agattttact tgattgcggc ataaatattt ccagccagga
    24721 gtcaattaga agttttccta gatttgacat aacaggcttt gatttgaacg aacttgacgc
    24781 tattgtatta agtcatgcac accttgatca tacaggcttc ttgccttcat tatttaaatt
    24841 cggattcgcc ggaccggtgt attgctcgga acctaccttg cctctaatgt atttgctgca
    24901 gtgggaatac ttgaagaata tgggttcggc cgccctgtat tcggtaagag acatcgagca
    24961 agtggttgtg cataccattc ctctttcctt cggaatcgtt acagatattt cccctgatgt
    25021 aaaactcatg ttgagcaacg ccggtcacat cataggttcc gcgttaattc atctacacat
    25081 tggaaatgga gatcacaacc ttctttatac tggcgattta aagtttggta agacatactc
    25141 gctagataat gctatatgga attttccgag ggttgaaaca ataattatcg agagcacata
    25201 cggcgataaa caagacatat tccctcagcg tgaagagtca aataaatgtc ttatagatag
    25261 catcaacgat accatttcag gagggggcaa gattttgttt cctgtgccct cagtgggctt
    25321 agcgcaagaa cttatgttga ttattcatat gcacatgaag gcaggtaaaa tcgacaaaac
    25381 aaagatgcta atagaaaaga tcattttaga agccagctct atacacgaag tttttccaga
    25441 atatctttcc agagaaatga accatagggt attagatacc gaagaagcac cttttcatga
    25501 agaggaattt aattttgtcg agactgcaag gcttgataac ggaccggcta taatcctttc
    25561 accttcttca atgctaattg gtggaccgtc ggtaggatat ctcagacaaa tctcaggaga
    25621 tccacggaat aaattaattt tgacatcgta tcaagcggct ggtacaccgg gccggtatat
    25681 acaagaaggg ggacgagaaa tttccgtatg tggccagact atcacaatca attgccaggt
    25741 gcagaaagtt gaaggattta gcagtcatag cgattataat caatcggttg cttacattaa
    25801 tagacttaaa cccaagctcc ggaggattct ggtcaatcat ggtgaacgag caagtgtaca
    25861 gaatctagca agctccttga acaaaatgtt caagatacct actcaacacc caatggtcca
    25921 agaagcaatt aagttgatgt gaagtccaga taatgaatag atatttgaga tagaggaaat
    25981 ttaattcgag aggtacgatt ttcgacgtgc gattacttca aatcgtaagt accacgccat
    26041 attctaacac ttggaggggt tataactaca cgctcttctc ccaatctagc aatatctccg
    26101 ccaactgact ggacatattt ataaagatcc tcgattgcct tgcgcagttc gacaacgtct
    26161 ttctgcgcca aaggggtaac tcgcaaaatg agaatcatgt gcttttttgc atcatctttt
    26221 atacctggaa tatcgctcat atctctaaga gttattgctt ttaaatagat gggcgtgttc
    26281 tgagtctgca ttaatctatc tactcttgtt ttcagaagac aactcgacgc aatctgcaaa
    26341 tagtcatggt ggtcaaaagt gatttactga gtggttgatt tagattcgta aactttctag
    26401 ctctccgtta tcgattggaa atgccgaaat aacgttgact tggccgtggt acttgactct
    26461 tacttttgac tcgtgtccac gtggagaatc ggaataacgc aaaactattt gtgaagattt
    26521 ttccaccaac gagtcctcgt aattcttgcc tcgcagaata gaagttggtc caggaaattc
    26581 ctctgcctcg attacaatgt cgtcgttaat caccaaagag ctcattatgt agttctcgtt
    26641 ctgatcccta ccaacgatca gctttgccgc atgtgataat ctgaagtgtc tgccaacctt
    26701 tagtaattct acatcgttga tacttggaac cttttctgga tcatctaaat attgcatcaa
    26761 atctctaatt ctcttagaaa actgaggatc tgttaacaga catccacctg ctgcatttgg
    26821 agggtcgtct atgccaaatt tccttgccag ggctagttga cttttacgtg acctccctac
    26881 gatgttacaa aggttttctc gtctgactga gccgtccttt tctatatctg tcatgggtaa
    26941 ggatttcgcc gagagtggtc ttaacacctt gccttgagta tctgtctctt tttcaattat
    27001 atccaaagcc ctattgttct ggctcatagg tctctggtaa agtacttccc ctgtaacaat
    27061 gaaatctgca ccaattttct tcatgtgttc cttggcggca ttgtacatca tgcttctaca
    27121 atcaatgcaa gggttcattc cagagccaaa tccgaacttg ggccgcttaa gcattctcag
    27181 atactcttca ccaaaataaa tcgtctttag aggaatttcg agatcactag caacttcctt
    27241 taccctatgc ccacatcctt taccgcaatc aaaatcgcaa aatggagttt taatcgctac
    27301 agcttcgact tcaacccctt gttctaacat tattctgact gctaagctgc tatccaaacc
    27361 tccagataaa agagccactg ctttcacttt ttttctcggt tcagcattat tggtcagctc
    27421 gggggaactt tgttccacat tattgtcaga cattggttgt tacccaggga gaggcagact
    27481 gaccgatata taaccttact agcagcaact cacagttggt aataaggacg atgctcttag
    27541 gtgccactta gattctgtgg tacggtagaa actgcccatg tcatttggtt gccttgtaaa
    27601 gatcaatgtg gtcaactcct gagctgtagg cagggccatg gcctgccatt taataaaatg
    27661 ctgtactctt ttcgacatca tcgcgattac taggtctgat atttctaata atatacactt
    27721 tttttaattt caacattagg cttttgttct ggggcacgcg atgaagctta ttgcataagt
    27781 gtttgttcag atcaataggt agatctgaaa tgcgaaatta ttttatcggt caaggtatcg
    27841 ctgactttaa caatgttttc ttgctcatct cgcgtcgggg taagtcatac gctctgtttc
    27901 agcaaagctg taatacaacg tcatggatca gaactagcca atatataaca ttgaataagg
    27961 acataagaga taatggatcc gttagataaa gcaattctcg ggtcattcta cctaagcagt
    28021 ttggacccaa gtgttgctgc tctagaccgg ctcagatctc gcttgccttt gcctagagag
    28081 atctataatg aaaaaatcca aaaacttgta gacctggggt ttgtcgaaaa aagtgacatg
    28141 cccaagttaa ctttcctagg cagagatgca tttaaggtag tcttggttgg cggagttttt
    28201 gacttaatac acccggggca catccatacg cttaaagcag caaaagcgca aggcgatgtt
    28261 ctcatcgtcg tcattgcaag aatctccacc gcggcaatga taaagaaaga ccggagaatc
    28321 taccatgatg agaggctacg ggcggaactt gtatcgtcgt tacgtcttgt cgacttggct
    28381 attttagggc gggagggcac gctgtatgag accgtcgaac acatcaagcc aaacgtcatt
    28441 gctctaggtt acgaccaagc tcatagcgaa aaggatatcg cagataattg tcagaaaaga
    28501 aacttgaacg taagggttgt aaggcttaat actccagtac cgggcacaaa aagctccgag
    28561 ataaaagatt atcttggtga atctttctat aggatttaag aatacctgag tgcaattttc
    28621 taatcgaatt gttcgctcaa aaggctagat taacattttt atcttgattt tgtctccatt
    28681 atggatccca accgtatcct ttatcgaaac cggtgctatt atttcgagca tactttcatc
    28741 atagtgagtg cgttccaata ctaaaacggc cgcattcctg attgctccgt catttatatg
    28801 agcaagatag cattttgccc atccatacgt tcttttagtg tcactgaacc catcaataaa
    28861 aatcgaagga tacttgccga tttcacgtcg ggagtctctg aagattttgt caacgagttt
    28921 cacgtttaat gtgccaggat acggttcgta ccctagtttc tcatagaatt gtttacgata
    28981 tcctcccaag gacatataat acgcaccctc gcccatgcct gaaacgactc ttccttcaaa
    29041 gctaatcgta gtaggagggg tcgattcaat agcagtcttc agagttgaaa acaatttttc
    29101 gattgctgaa tatcctttat ctgtcaattt tactctgaat ttctgacccc ttctgaccct
    29161 gtttatatat cctgcagcat ctaactcctg caagtgttta gaagctgctt gttgtgactt
    29221 cattatattc ttacctaaat cagtcgtagt caattcgaca aagctgtatt tggcgcctaa
    29281 caggaggagc tcggtcatag ttaggatgtg ttgtaactta atttctgaca tcctatctag
    29341 ttattgattc ccatgtcgcc aaaagttctg acgcgaacgt ccgtagattt tttcaaatcg
    29401 gccgttctat ggccgacgat atattcgatt ccgactttat cagcagcatc caccaggcgc
    29461 tgtgtgacaa tgccgtctaa taccagcatc tttgcccctt ccgtcctatc taactttttg
    29521 accacatcgg aaacaggaac tttaagtagt attttcatcg aaccatccaa tactacggct
    29581 tctagggttt cgttaatttg tggatagacc tccttgacgg ccgaaagaat atctgcatta
    29641 tcttcagaga cggttacgct cgagccgcct gaattaactt gtatttctcg ttcttctccc
    29701 atgccttgtt cttgtctgtc tcttttagtg ttttgtctcc tttccgtctg ttgctgctga
    29761 ggtatgatta cacgcccttc ttgcccattc gcacctgcaa atagcattcc ttctttcaat
    29821 atctctgcga tctccagagg cgtgcattcc tcaacttctc tgcccgtggg ggccctcagc
    29881 actttatcga tatttaccac gccatgtaat tccttaagaa ttaagtctcc tgctcgatcg
    29941 ccgtcgagga aggcgattac tttcttgcca tcagtgagct tgatgatggt ttcatcgatt
    30001 tttgctccct cgatggcaat cgcgttgtca aagccggcac gcaacagatt gatcacatct
    30061 gcacgccctt ctacaagaat aatccagtca gaatcaaata ccccggttcc gcaagcgagc
    30121 tgagcctttc caaaggcagt aagcttaccg ggtttgctgg cgtcgtaaac gtctttaagc
    30181 atttcctctc cttcgctgat agtctttgta gcccactctt gcacgatctt cttggccctg
    30241 tcgacaataa cttttttctt tatcgcgcgg atatcatcga tgccactcaa tccgaatttc
    30301 gcttggaacg gcccaacctt gtcaatgctt tcaatagcag ctgctattag tgcggctgtt
    30361 gaaatatcgg tgctcatggg aattacagca tcgccctttg ccatatttga cttcgtgtcc
    30421 acattaactt cgatccttcc tacctttgag accttctgta attcgttcaa attcatttct
    30481 ggtccaagaa gaccttcggt ctgcccaaag atcgctccaa ttatatctgc tttctcaaca
    30541 agtccatcaa cctcgaactt tagtttaacg tgatacttca cgatacccgt gctaggcaat
    30601 ttcataacct catatttact attaagtaaa tttatggggg agtgggcttg aatataagtg
    30661 ctttgaccac gtcacttgga tatagttgtc taattctata tgcccttgat gcgttcacat
    30721 atagaagtac aggtcttttc gattgccagt aattccagaa aaatatggcg catatgccac
    30781 aagatcctct acatgtctaa tcttgccatt acatatcctt gctagcgcac ttttgtagaa
    30841 taagctcacg ttatttccgt tgaactgcaa acgcttcagg agtttgcttg ttaaatgctt
    30901 tcctgttcta tccatatcaa gaagcaaaat tatctttttt ccaaacgtac aatggttatc
    30961 cacaaagtct gctacgcctt taaaacgact gaatactgtc aaggttccat taaaccccac
    31021 actagccaaa gctcttgcat ctctctttcc ttctaccact atgaccgatc cgttttctga
    31081 ttcttcgtta agcatgctaa taaagccgcg aagacggtaa acaatattat cgtctataac
    31141 cttgtaccgc ataggctaaa tgttattaac agtaaaaaca tttaacaatt ttctcggagt
    31201 cttagagaag tcgtaaaaat atttcatctt gactctggtt tcacctgtgc ttaccgaata
    31261 aaagacgagc ttgtgaacat tatgatacat gccctcacat atggccctct tcggatatct
    31321 atccgaatta tctagatatc aaagactcga tcttggtggc gcagagaggt catctctttt
    31381 caagataatc aatgatcaca atcgagtcaa tagattttct tcttatcttt gaagccacaa
    31441 acaaaggacg aggatagaaa gacagacttc tggagttcaa tctcgttaat gagatagttg
    31501 aaggaagatc ttatctcttg aggagctagc gtaatgaact attcacctat attacttgta
    31561 aaatataagg gtaacatggt attgaaacaa atactataaa gagtgggagt gtacatttcc
    31621 attccattac taccagctat ctcagaaggt gctccgaaat aactcattac tctcgcggtt
    31681 cgaaagaggg agaatggctt aaggagacca atgtaaagca actctatgat gagttaagat
    31741 ggaatgccaa ggtccttggc ttcaggcttg ccgagatgta tagtacatca aacatgctta
    31801 tgagcaatgc atataacgag acaaacgata gctccgacga tggaatatat gtagtcgata
    31861 gtgcaatgaa aactgcttta tcaaaagata acaagttcat gcccctgata aacgttatcc
    31921 agaaggagaa ggttccaagg acttagtcca attacatgac agccattgaa tcgcagcatt
    31981 gtaaactgga cttgttttca aatacaacat aaaaattagc ggcacgaaag cttaattcta
    32041 cattgcatgt ttctctctct tgcatcgtgt atgatagaac tcctaatagt ctgattccaa
    32101 gttaaaggaa tgctcattaa gacatctatg gatcgattcc cttcttcaca cgcattgcat
    32161 caataagacc ttctgcataa ccaatgctca gaacagccaa ttcaggtttt ccctgccgta
    32221 ggaatctttc agcgtcgtca atatagcatt cagcgttggc tagaatttcc atattgatct
    32281 tgttaccaga tatcccatta tctgtgctag aaatgcgttt cagctgggat agtgcttgtc
    32341 tagctttggg agcataacgt gcaatcatgc cggctgaaag attctgcata tttgcggtat
    32401 tatccaatgg ttcattcaga ttgtaagtca gagtcattag agcctcgact tctgtaaaat
    32461 gcaaagcacc ggtgatgatt atggaatgcg gtccttgtcc aaagtttata ccattaagtg
    32521 atctaaccgt gccggataca attttttgat cttgcattcc gattctagat gctacaattg
    32581 caaatgtatc ttcgaagaaa accttgaacc tgaagtccct ttctgcttgc aacagggttt
    32641 taaaggccga atttggattt agaaagaaca atttaccgtg ctcattatta tactcagtca
    32701 atatcagtgt atggtttctt ccgagtaaat tctcgaaaat tgcattgtaa acactaacag
    32761 ttgataatgg ttcagacgta attgtgacta tccttccaaa cttgtatatg tggagaccgg
    32821 cctcaccgat taaggatgta atgccagatg cagcgtgaat gacatctacc tttattgaat
    32881 ttttgacagc cctcacatag agttctatca aggtagtagc gatgaagggg tctccatatg
    32941 ttaacagaac taaatcactt gttcgtgcct gctcaagaat atcccgtccg tcttcaacaa
    33001 accatctttc tgcaggaagt atattgatct caaaatcgtg taattctgca agtgacctca
    33061 attcctttag atcatcgtct gaaacaaaac ctgtaaattt atccaagtat attgtgttac
    33121 agttcctcaa aatctctagt acatgagtac tcatagtttt gtagccgtta attcctagac
    33181 caataaatcg aagcatacga ggaaaatatc tcctccgttc tatattttat cctcagttaa
    33241 ggtctgggtt ttggtattgt tatgaagtct tgatatgtga aacaaggctc tgctgaatac
    33301 atcgatgcta gagatgtatt ctgtaatttt cacatgctca tttgctgtat gcgaggaatg
    33361 tggctccccc ggtccatacg taacgactgg gacattgaat gcagttccca atacgttcat
    33421 gtcgcctgtt cctgttttgc gtacaagaat agcatgagtc ttacaaacct cgaggataga
    33481 aagagacaga gctctgacaa ggggtgaaga atggtttgct tcgaacggtt ctgttttgtc
    33541 ttctattcta tacgttgctt tgactgattt gtcagcggca accctgtcaa tgactccttt
    33601 cagttgctcc agtacgatgt cgcaactact ggtcgtagga atgcgcatgt ccaccgttat
    33661 tttacatttc tgaggcgtga cattgtggct ggagccgcct gtaatttctg taagactaca
    33721 acttattgaa tttgccctac tcctgctgtc atttccggcc acagagattt cggacttgat
    33781 cgccttccaa aagtcatata tctcttcgat tgaatttttt gctagccaag gagcgcttgc
    33841 gtgagtgctg ttgcctacgt cacaggtaag tctaattgct agtctacctt tgtatcctat
    33901 agtgatattt tgtgtcccac ttggttctcc gaaaattgcg tagtcaaacg gaagtttgct
    33961 ttttacgagc tgcttgactc ctgttgcatt cccttcttcg tcaacgaccg cagcgaatat
    34021 gatggtgcct ctttgttttc gaaattcaga agccgccaac aacattgcga ttagagcggc
    34081 cttcgcgtca gatgcacctc gcccataaat ataaccatct tttaccatta cctggatttc
    34141 tcctgggaca gtgtccatgt ggccgcagag cattattttg ggatcgcctg ctcctttaat
    34201 ggccataagg ttccccgcac tgtcaatatg ggcctgctcg aatcccagct cgctaacgca
    34261 ggtctccttg agcatgttcg ccaacgaagc ttctgatcgc gaaggcgtgt acaactgtag
    34321 agtccgcagc aataactcga cagcatagtt aggggaaacc aatttgcgtt attttccaag
    34381 attggacaag tagtcgatca ttaatcccgg tatgtcaaca ccagtcgttt taaccgtatt
    34441 cttaaactct gttgtgttgt tcacctcgtg aaccatcaac ccttcttcat cgctttccat
    34501 taaatcaacg ccgacgatct tcccatccat cgcccgtgaa gcttttatgc atatgtcctc
    34561 taactgcgtt gacactggac attgttctgc tcggccgcca agtgccatgt tggtcttcca
    34621 gtctccttca ttagaatatc gataaattgc agctacgaca gcatcgccta ccacgatagc
    34681 tctgatgtct cttggggggc gctttacgaa ttcctctaga taataaatat gataaagcgg
    34741 aaacatatgc atcctatctt ctatcactgc cttggcagca tctttgtcac gcaagagagc
    34801 aactagtcga ccccaactcc cgatagtggg cttgataacc gcaggatatc caaaattgtc
    34861 gagtgcggcg atggcagatt cttcagaaaa agcggaaata gcttttggtg tttttacacc
    34921 tgcattaatg agcctcatat gggcaaataa tttatttccg caagtcgcgg ctgcttgtaa
    34981 tggatttacc acacatgctc cgagaccttc cagtgcagca gttgaatgaa gacttttaaa
    35041 atagctcaca caacgttgaa caatgattct attcttataa ctagaattct tttcgttaag
    35101 atcgatatat agatctttac aattaacatt gtcgacttgg attccacgtt tttttgccga
    35161 ctcgtatagg gccttttctt cccatctcat attgtcgtaa agaatggtaa gagaagagat
    35221 tggttttgtc cctggtgtca ctcgccccag tcctcgccga caacttgggc tggttttata
    35281 tcaaatttgt ctccaacttt tactaactcg tagcttgcac cgcagtcagg gcaggttacg
    35341 atttcacctt ctatagagtc cgaaggtatt ttgatttctg catcacattc aggacactta
    35401 agcatggcac tacttacctc ctaagccttt acttaattta tcttaggagc agcatcatta
    35461 agtatctttc tctcaagttt ctcatcgaga tacaattcca agatgtctcc catgcgaagt
    35521 tgctttaatc cacgagctag agtctcagct tcatgaccgc tggtttctag tcctagcatc
    35581 gttaacaagt atgctgcacc attcttaccg gcaagctcac caaatacaat cttgcgtcta
    35641 ttaccgacag ttttgggctc cagaatttca tacgctgatg gattcctcaa aattgctgca
    35701 agatgggtac ctgctttgtg tttgtacgcg ctgtcaccta caactggctt cgctgcggga
    35761 acttctacac ctgtatactg agaaattgtc ctcgaaagat cttttagcat gtgtaagcgt
    35821 aggtcgttgc ttgatttgta aatcaacatc agagcgacgg cggtttcagc caatgtagga
    35881 atacctgttc gctctcccaa tccgtctatt gtggtgtgaa tctgatccgc tcctgcatca
    35941 catcctgcta acgcattagc tagggctaaa cctatgtcgt tatgacaatg aacatctaag
    36001 gagctttttg acatatcaat actttcgcga accatcttta caaggttgtg cattccggtt
    36061 ggtctcataa tgccaacagt atcgggaata ctaattcgtt ctatacccag acgattaatt
    36121 tctttacaca tatctactaa gaaaactgga tcagtcctgc tcgcatcttc cattgtgaat
    36181 ctagctttca aaccatgaga cttgatatag tcaacaacct ccagcgctct aattttggcc
    36241 tcttctcggg taatctttaa ttttgctgag agatggatat ccgaaattcc catgtaagtt
    36301 gcaacccacg tcgcaccaca gctgatagct atatcaacgt cagatttgat agcccgcacg
    36361 tgagctagaa tatctgctct gagtccctgt cttattatag ttctcgtggc ctctgcgtga
    36421 tcgggcgaaa caaccgggct aatctctatt tgatcaacac caaaagaatc gagcatccac
    36481 gcaatctgta ttctctgttt cacgctgaat gatactccag gatgctgctc accttccctc
    36541 agcgtagaat ctagaattct tattttcttg tttgcgctat cggactcatt atagatgtgt
    36601 gcatagtagt taggatcctc cgaatttagg gacatcgata actagattcc gcttatttcg
    36661 tagatgatat aaatgttatc gcaactataa tagaaggttt cgacaatttt aatagcgctc
    36721 ttcgacgttt aggtgcacct cagtctgaat accagaggga aaaattctaa tcgacgagaa
    36781 gatgcaagac taccgcagcg ttttgagcac aatcaccgta tttgtatcag agacccctac
    36841 agtctttctg acatcgtcta tgcatttatt aatctcgcta atagtgggag cggcaagaat
    36901 cgccgcaatg tcatattgtc ctgtgatttc gtaaattacc tgtactccgc tgagtttctt
    36961 taactctgaa gaaactgagg aggtatcaga tgtggaacta actgatacca gcgttatcgc
    37021 gctcgtcttt ccgcttgcac ccaactcgat tgtaaattgc gtaataactc cattatttac
    37081 catatttttt acgcgcctcc tgactgcaga ttcggataac ccgatctcgc cagcgatttc
    37141 gacgaaaggc cgtcgcgaat ctgcttgcaa aatccttact atcttttcat ccaaagagtc
    37201 aaggtcagtg gacatgccgt ctcttctcct cttctgtaag aactagatca attatttgga
    37261 ttgcgttcga gactattttt tcatccatta caagtggcgg aagtagcctg ataatattcc
    37321 ttccagagta taacatcaac aaaccttttt gtagtgcgtc aagcaaaaca tccctaactt
    37381 caaaacgcaa ctctaccgca agcatcatac caagacctct aacctctcta atgattcgat
    37441 gtttctcctg tagcataagc aggctttctt taaaatatct accagtcttg tcagcatttt
    37501 caaccaattt atcttctgtc aacgcctcaa tagtagcgta gcctgctgcg caagcgagtg
    37561 gatttccaga aaaggtagac gagtgctctc ccgttctcat acattcgata atttcagcct
    37621 ttgcgaatgt caatcccata ggcacaccgc ctgcaatgcc cttcgccaga cacattatat
    37681 cagggacggt attccagttt tgtcctgccc acatcttccc agtcctaccc agcccacttt
    37741 gaatttcatc gaatatcaag actaatttgt gtcgattaca tatttcgcgg atactttgga
    37801 taacaccgtc aggtggaact ataattcctg tttctccttg aatcggttcc aaaataacag
    37861 cccctatagt ttcgtcgatc gcatcctcca ctttgttggg atcggcgtat ggaacgaatt
    37921 ttatgccctc taagagaggc tgaaacgatt ttcgatattt ttcgttgtgt gtgacagaaa
    37981 gggctccgaa ggtcttccca tggtacgcac cattcatggc gatcactccc ttcttgcctg
    38041 tgaacttgcg agagaacttg agtgctgcct cgactgcttc ggcaccacta ttgctgaaaa
    38101 aagtcttgtc caagccagga ggtgttatgg tggccaattt ttgcaggaac ttaagtcttg
    38161 tatcattata gagtgacata tggcatacta ttaatttctc tacttgggtt ctgatagccc
    38221 gcacaaccct gtcattacag tgccctacaa tagcgacacc atagcccccc atgcaatcaa
    38281 tatattctct atcagaagta tcccaaacta ttgcgccctt ccctctcgca atgttaaccg
    38341 gaaatctttg gtaaatgcga gctaaatact gatcttcgcc gttcatttct gagtgatcac
    38401 tgtacaatta tcatgcgcaa tcgcctttga gaggggcttt tgtgtttgac ctgaggcgat
    38461 cacagcttca ttcactccca tttgcaaagc ctcgatagca gctaggactt ttttttccat
    38521 accaaatcct attcgtggaa gggccgcttt cgcctgctca actgtaatgt gtgttaccaa
    38581 tctatcgtct agtctaaggc cgttgacatt tgtcataaat ataactttgt tagctttcag
    38641 agctcctgct acgtaagcag ctgcccggtc accatcaaca ttgagaaaat catattcctc
    38701 acttagggcc accggcgata taacaggaag gtattccttt tccaacaata aagttattag
    38761 tgacgggtcg acagaattta tcttccctgt atatccgccg tcaatcacca ttttcctacc
    38821 tttctcgttt attattacga gcttcttctt gcggtctgcc ttcaagaccc cgccgtcaat
    38881 tcctgatata cctgctgctt ttatgccatg ccgcagaagc actcttacta ttaccttgtt
    38941 gagcttacct gccataacca ttgtgtagat ttctgcagtt tccttgtctg tatacctgct
    39001 tcttacgcca ccgggtgaca caacaaaccc ctgcgtctta ccgagtttgt tagctgtgag
    39061 agtgacttcc tttccgccgc catgaacgac tactaattta ctgatttcgg atgttttttt
    39121 tatatcatct attgttgtag gatggagttc atcaattata cttccgccga tcttgactac
    39181 aatcatactg gtgtcagagg agtatatttt agaccatccg tctcgttaaa accttgcatt
    39241 acattcatat tctgaaccgc agacccggct gctcctttca tcaaattgtc agatgccgac
    39301 agagcaacaa ccctttgatt atcgtcgtca atgtcaaatc ccagatcaca gaaatttgag
    39361 cccacaacga attttggatc cggaaatttg tataagcctt tcttgtcacg gataaaccta
    39421 ataaatggct cattcttgta cgagtttcga tacattttcc agatatctaa ttcagaagct
    39481 cctttagtga ggaacaaatg attagtagta agaattccac gaactaggtt tactgcatga
    39541 gggctcatac tcaccttaat agccttctta gcaattaatc ctaattcctg ttcgatttct
    39601 ccagtatgtc tgtgcttagc cggcttgtat ggtcgtatta ctccgtagcg cattgcatga
    39661 tgcgttcccg aacttagctt ggcaccagca cctgacgagc ctatcttgct atcaactatt
    39721 atgtgctcag tgtcaataat attattttct aggagcggct ttatggcgag caaagaggtc
    39781 accgccatac agccagggca tgaaaccagc tgtgctcgtt caatatcggg cctatgaaat
    39841 tcaggaactc cgtagacaga ttttgccagt agctctggga aaggatgctc ccacccgtac
    39901 cacttgacat aatccttagg attctttaaa cgaaagtccg cagaaagatc gacaatcttc
    39961 ataccccttt cgtataattc tttcacaatc tcgtttgatt tgccatgggg aacagatgta
    40021 aaaaccatat cgcaagagtt tgccagtttt tcggtatcca taggtgaaaa ggtaagatca
    40081 atgaaaccct tcaaactcgg atgaacacgg tgcacatatt ggccggcttt ttgtctggaa
    40141 ataaccatac tgatctcgac tttggggtgt gacactagca gcctaagcaa ttctccgcca
    40201 acatatccgg aggcacccac aacacccacc ttcaatttag agagatctcc tgtattctgc
    40261 agaggcccga tcttatgaat tttgtcgtat aaataaagtt cacctggttg aacaggttcc
    40321 taatatttaa tttatatttt ttggtcataa gtgttcattc taggccacta gtgaccaatg
    40381 ttggtctact tcttaacaac cgcgacacta taatctatta ttgcactcgc aatatcggta
    40441 cttgaaacgt tagacgctcc ccgaaactct acagtattgt taatttcgtg aactaacagc
    40501 cctctttgtt tatcttccat taaatctacg cctagcactc ctccgcctac agcccttgca
    40561 gcgcgcagtg cgatctcttt aagttcgttt gtgataggag cgttctcggc cttccctcct
    40621 cttgctacgt ttgttctcca ctcattctca gcagaatagc gatataccgc tgcaatgatc
    40681 tcgtctccta ctacaatgca tcttaagtca cgcggaggcc gttcgaccat ttcttgtata
    40741 taataaatac ggccgagtgg attatcgttt tcctccctca tctcaattat catattagct
    40801 acctcctcgt ctcgtaatgg ataaactcct cttccccacg atcctacgat cggtttcaga
    40861 acaaccggga atcctgtcct attgatgact tccatcgcag ctccggcact gtaggcaaag
    40921 taggttttag gtgtgggcac cttgtgtttt gcaagggtaa gtgaggtcag caatttatta
    40981 ccgcaagtct cacctacttt gaacctgttt atcacagaaa agcccaaaaa ttctatacac
    41041 gccgtaagat ataggcctct aaaatggctt atacttcttt gcaagattac atcgcctaag
    41101 gcaagttctt ttcgtttgct atctgttgag atcgtgacgc tttttgcgtc aatcattcgt
    41161 gtctttattc ctttttttaa agctttatcg taaatagatt tttcttcgaa gcgcactttg
    41221 tcataaacga cgcaaagttc taccatctat tgcccccaat cttcacccac attttcagcc
    41281 ggtttcagat ctaccatctt ctgagccttc gaagcgatct caaaatcagc accacagtca
    41341 ggacaagtca caatttctcc tactagtgca tcatcaggaa tatttatgtc agcaccacag
    41401 tcttggcatt ctttcttcaa ctacctgctc cgcctccatt tttgttgtcc tcgaatcgct
    41461 gcctcctgtc tatcatattt gcaactgttg actgtagacc ccaaagctct acaaatccct
    41521 ttgctaaact ttgatcaaaa gtcgatccag ctgtgtacgt tgctagttca tttctataga
    41581 gagaatgttg cgattctctt ccaactacac gaagcgatcc catctgcatt tttaatttta
    41641 cctttccgct aacctttttt tgggttgcat ctataaactt atccaaatgt gatcgcagcg
    41701 gatccttcca taaaccagaa tagactaacc aactccactg ctcatcaaca acacgtttga
    41761 aacggagttc atgactattc aaaaccatct tctccaaatc tttgtgagcc tcgacgatta
    41821 cgacggcggc tggagcttca tagatctccc tggattttat tcccaccatt ctgtcttcaa
    41881 tgtgatccac aataccgact cccaaggatc ctgctacgtc attaacgtaa cctatcaagt
    41941 ttataatctt catgcgttgg ccgtctactg caacaggaat acctttttcg aattctaatt
    42001 ctaaataacc tgctttgtca ttgtcaaatt ttacgaattg aaatgcatcg gttggcggtt
    42061 cggagttagc atcctcaaca attcctcctt cgatcgccct gccccagata ttaaggtcaa
    42121 cactatattt tttggcttca gaacttatcg gaatatcttg ttcttgagcg aatttcattt
    42181 ccagatcccg tgtcaaattc atatctctaa tgggtgcaat aatctttaga tgtgggtcga
    42241 gagcccggat ggtgacatca aacctaatct gatcgtttcc tttccccgtg cagccatgag
    42301 caacggcagt ggctcgaagg ttgtgcgcct cttcaaccag cttagctgaa atgaggggcc
    42361 gagcaagagc ggttgccaat gggtatttgt cttcgtacag tccatttgat ttaatgctgg
    42421 gaattacgta gttgtcggca aactcttcct tcccatctac gaaaatgtgt tttattgctc
    42481 caatagctaa ggcttttttt tcgatcgcct taaaatcttc attctgtcca cagtctactg
    42541 ttaaactaat gacatccaac ttatgcaatg tttgcaagta tcttatacat accgaagtat
    42601 ctaaaccgcc agaatatgct aggacaactt tctcgttagg catctattac gcgcttcgag
    42661 cgcccaacgc tacatttata ttttttggtc atgactgacc tgattagtga tctattgacc
    42721 aatgtcggtc aacggaaagt cgataactca tgcagtaaaa gaggttatta ataatgatct
    42781 ttcttttcaa gactctctag aacggggtta ctgcaatatt agtgcggtgg caaggataat
    42841 cagaccacaa gtgaaccgac tcctaggcag aaatattagg atcgaaagta ttatcaccgc
    42901 tcttaaacgt tcacggaagc tctccgatat tcccgtacga cccatgttga ctatcctagc
    42961 aaacagtact atcagcgtga aaactgacgt cgcaaagata agtactggaa agagcaagag
    43021 aagtatcgag aaggtcgcaa aggccctaac ccaatacggt gacaatttta tcagtgtttc
    43081 agagagtata ttatcaacaa ctatcgtatt tgacgacacc ttactccatg aagtaaaagg
    43141 aattttcgcg aattataaca tattggaggt tgaggatgat ttggctgcaa ttattgttaa
    43201 tagcccagaa caaattatca agacgccggg ttgcgcaatc gctttttaca atcagcttgc
    43261 tagaagacat attaatattg aagatacagt tagttgctat accgatacca ttatactagt
    43321 aaagatgaca gatgttggaa aagcatttaa tgccctaaca gaactcatct ccagttctcg
    43381 caaatttcca aaaacagaca ataaaatttt aaaaagacac taaaattttt tattcgacag
    43441 aagggttgac gagtttatgt acagacgcta taccttggat gaagtaaaac gtaaaattat
    43501 cgatgcttta caagacaata gtatcggttt atccggcgtc gagcttgcgt caacaacggg
    43561 cattaatagg atgacaatta ccaagtacct aaatatattg gcgacgatga gattaatcaa
    43621 gaaaaagagg gtaggttctg taaatgtgtg gtttgtagaa ccggggacgg cagattatga
    43681 attcccggtg gattatcttg aagtccaaca aaaatttatg aatgctatag tgatcggtga
    43741 cggggatcag gctcgaaaca tcctgataag tgtgattaat tcaaacatcg aattattaag
    43801 actattaaca gacatcatag ttcccactgt aaacactgtc aatgagttat acaatagggg
    43861 tcgtctggga aagaccgaac gaatttatct attaaatttg atactggaat tgattgattt
    43921 attgaagttt accatccagt cagacagcag taaacgtggc gcttattcct ttttcgtgac
    43981 ggcatctgaa gaccggatct atcacgcaaa aattggagca cttgcattgc agattttggg
    44041 attcaaaaca tcgtacatcg gaaatgttga acagcacatc gatccctttt ttgatattga
    44101 tttccaacgc tacataatga agctatggga taagaagaaa ggtctattga tagtatgcat
    44161 ttactcctcc gaagaaggct cattgcgttt tttgtatgca gctactagag caatgaaggc
    44221 gaagctaaag gcagaagtta ggatagtaat atttacaaca ccggagcttc gaaatactct
    44281 cgaatcttta ggtaccgact tcattgccaa tgatctgaac tctcttattg agtgggtcga
    44341 aagagaatat catcggctga aactatagag tcagtcggct atcttggaaa atggcataat
    44401 agtgggtgga gcagttaggg ctaatagacc tgctagcatt gccgtctgcg acgctgagat
    44461 gaagaattgc tatcgccaca ggccggaccc cgcctgtcgt aagacctttt ttaagatatt
    44521 gctagccacc gataaacgcg ccgaagtgcc aatatcgtaa taatgctgtt ggcatattgg
    44581 caatagttag tacaagagcc tagaaaatcg ggcccttaca tctagaaaat acgggcggca
    44641 gttcgtagcg ttgggatgcc ccgggacgac gggcgcttct atggtagtct agatgcttct
    44701 ctcggtatac ggatctgtct tctcaattgt cgccgattag tcccagattt tcgatcttgt
    44761 cataaagtgc atttatctgc gactggattt ccaacttttc gacttcggta tttacagcat
    44821 ctttcttttc gtacaactct ctaatttgtt tatgcaagct gtatttgatt accccgatcg
    44881 acggattcat gtctcctcaa atgataataa aaaattagat agcattaatt gtcaaataat
    44941 tttgcctcgt ttgacaaatt tcaagctaat aaaataatat gcaatatgct cctgtgatat
    45001 ctaatgctat actcacacat ccgtcaacca aatagaaaaa acccagtggt gcatgatttg
    45061 agccttgcgt atcattataa ctatgaccgt actcgcttcg tagataaaga atagcccagc
    45121 atcacgagac tagcgatcaa atgaaaatgc cacaaatact aataccaata ataataataa
    45181 taataatatt tttaatcaat ttgttatcta tttgctatca cttgactgat atacgagctt
    45241 ggaaattcac ttgtttattg aatacaattt tttcaaatca aacaataatt ctggttgatg
    45301 atatggtaat gtgtatactt gactaaagta ccaaatgacg ggaaaaaatg aaaatattga
    45361 tcgccgagga cgatagggat attgtattat tgtataagca cacgattgag aagagacatc
    45421 atgaggtcat aactagcgaa gatggggaag aatgtctaac tatttatcac gaagaattcc
    45481 agcgcgctag atttggaaag cggtacagcg taaccaagac gcctcagttt gatgccgtgg
    45541 tccttgacca taaaatgccg aagattaacg gaatggaagt tgcaaaagaa atcctagcaa
    45601 taaatccaca ccaaagagtc atcttcgttt ctgcatttgt caaagagact cttatcgatt
    45661 caataaagaa tctaatgcag gtggtagagg ttatacaaaa accatttcat ctgactactt
    45721 tagttgatac tatcgaagat agagaaatct attcagaact acagaatctc aatgtcgata
    45781 ttgctgtagt taaagctctt aaacctaccc acgagcagat aaaagatctg ttagatcggt
    45841 taagtagagt tcgggacatg caaacattcg tgacatccga agggtaacta ggcgcacgaa
    45901 tagctaaaat gatgttagta gccgttggat catacgttgg aaaaatatct ataacccttg
    45961 gttagttcat tccttgcaga ttctgtaatg aacaaaacac agatgatgat tctactcagt
    46021 gtcagcatac tagtcttgct tatttctcag tatgtcacga atagattggt tggtgttgtt
    46081 agcgcgttct ctacaattca agagagacat ggagcacgtg catcgctatt tgtttatagc
    46141 aaaacattgc aactggcatt ggagagtcaa ctcctacctt ttcctcaaag cagtagtcag
    46201 aattctgata tccattatgt tagtttggca aacctcacgg cctcaccact gcttaccgat
    46261 attttcaaaa gagtcgagaa ctcggtagtt cagataacaa gcagagtaaa cgacaccggt
    46321 aacctgcaaa ttattataaa cggcaaccct ttgcaaggcc cctctactag gttgggatca
    46381 ggatttgttt acgataatca aggacacatc gttactaaca atcacgttgt gaacggtgct
    46441 aaaacagttg atgtaacctt cgttgacgga aatacatata cggcaaaggt aataggtact
    46501 gacccttcta gcgaccttgc attattacta atcacagata atttctcagt tgaaagagtc
    46561 gttccgctta taattacaaa ctcatctagc ctacaggttg gacagcaggt aatagctatc
    46621 ggcaatccat ttggacttag cgataccatg acaactggga ttgtaagtca aacgggacgt
    46681 ttgctgccga acccagatac ccaatttggt ataccaaacg gaattcagac tgatgctgca
    46741 attaatcctg gtaattcagg tggtccatta ttaaatatgc aaggacaagt agtaggaatg
    46801 aatactgcaa tcagttcttc gacgggcgaa ttctcgggga taggatttgc aatcccatca
    46861 aacgcaatga cgcgaatact gccatcgctc atccagaccg gaacttatac ccacccctgg
    46921 cttggaatat ctggcggtag cctaactccg gccttggcac gaaatgcagg cttaccaatc
    46981 aactacaagg gagttctagt ccgctcagta caagtaggca gtccagctga aaaggccggt
    47041 ctgcatgggg tcagccagga tgtaaattca aatccacatg tcggcgatat aatcgtagca
    47101 gttgatggtc acaaagtaaa acgtattgat gatatcatta attacatgga aacccaaaaa
    47161 tctgtcgggc aaaccgtgaa acttacagtg aatagaaatg gacagttcat ggatttgaca
    47221 gcgacgctgc aaccacgccc atcgtcacta cctacccctc ctcaatgagg gtattgagca
    47281 ctgacttcgg ttgattgact gccctgactt tagcagacga gagtagcaag ttggtccgat
    47341 gctgcgtttc cttgctttta aacagatact gagaattcgt cgatacgaga aggcggtcag
    47401 agatatatct aactgtctgc tttctaatat gatagtaccc caaggacaaa acaggtagta
    47461 ctacaattcg gtgctactac tatccggtgg caggcgtaag atatctcgaa cgaggaggac
    47521 agatccggca aatttcaacg ataatttact tgctcaaata ctggtgcata gcaagtgatc
    47581 ttgcaactcc cttcttttaa tgacatgatg gtcataagat gtgtggttga cgttgcagct
    47641 tctttagcct tgtgtttttc tcgtatgcgt agtctgattc ttcgaagtgc aaaaattttt
    47701 tagaaacagg catttgtagc gaaggtccgc agatatgttt gaccgagttc gtttgggtgc
    47761 attgttttat ttggagaatc acgcggccga cttcattaag tcattatacc tgtcagacca
    47821 agaatcgact ctaatagtag cggagcaaat gtcgggacgg cccaagaata tttttgtttt
    47881 ccatcgtgac agagcagatg gttttagatg tgaagcgatc cacattattt ttataatcta
    47941 tgagtgagaa aggcgacact tcaagtcaag aaacatctac tcactgggct gaaataatgg
    48001 gtcaactcgt tgacagggtg ttagaaaaga acatggcgat gacatataac tttgacgatc
    48061 aaacaatcga tatgccaggg gcacaaggac caggcggtcg acattgcaaa tatacaacct
    48121 gcgttaagtg gaccattaac ggcaaggtaa taattgcgac agaagcgtac aaaaaataag
    48181 aggttgtgat cggatccaga aaaaatgcta tgccatgatc aagtgctgag cgggaacgga
    48241 gtcacagttt gatttcttcg aaaaggtggg gaaccgataa ctttgggaaa agatgcaaag
    48301 ccaacgttat cgaaattgat cacctgaagc gacgatatgc aaataaagag tgttaaggca
    48361 actagcaatc ttaagagcga cctgaaaaca gattaacaac taattcttaa tccgacaatc
    48421 ctactcttag gatacgggga taataattcc tacttcacgt tcacattttg atgtttccga
    48481 gctaggatat agtatgataa ctgctaccaa tattttgcct tgatagtttc aattactcgt
    48541 tcgtgttccg ggcccttctt ccttgcatat ctctccattg cgccgagcgg caccccacct
    48601 aatgatctgg ccagcgctgc aaagaagagg ttctttatcg ggttctcacg acctaccttg
    48661 cgcataaact tctgaatccc atgcttgaag ttgtgttgcc atgtcttgta cattacccct
    48721 agttttgaag catcaattga attgccgata tcgaaaaatg cggatttctc caagagccct
    48781 attgggacaa aggtaagagg agtcaccgtg aacttggaat ctggctgttc gttttcgagc
    48841 tcatggataa gccttatagt ctcccagcta tcttctgaag tctcgtcgtt attcagcccc
    48901 atgattatgg tgaaagcagg tacccagtag ttttcgttga gagtctttac ggcattcttg
    48961 acaacccaat gccattctgc tggttgataa ggagctaatt ttctgtctgc gtattttcca
    49021 atcagcctaa cactccctgt ttctaatccg cattgaatgc ctatatgatt agatgggcta
    49081 gctttgataa ttttagatac gttaggaatt agtctttcat cagcgattgc tccagcaagg
    49141 gttccatgtg tggggtttgt atgttcaaca cctgtggcca tgataccttt gaacagctcc
    49201 tcgatagctt ctctgtttgg ctgcatattc tttgttgtcc ttggatttaa cccataaacg
    49261 aaaatatcgt cactatgaac ccatgcattc tttaacccac catatttcat gttgatttcg
    49321 atttctttct ggaccttctc aaccggataa tatctcaatg gtctgagagt cacatcgcag
    49381 aatttgcacc cacgaccaca gcctcgcata acttcaactt gaccatgcat gcttggatta
    49441 ataatattcg gaatctcgtc aacagaaggt tgatcccaac agtggatgaa tctaccatgg
    49501 tattttctgc catctcttgc aaattcctta atctccactt tgaattctgt cttcttgaat
    49561 ggattggctg tttccatctc tccattaata agggcatcaa agaatctccc accagtgcca
    49621 tcgatctcag gtcctatgcc accaagatcg ccttcaacaa taccataaag atcatattct
    49681 tcaatttttg ctgggtcata attatattgc caagttccag aagcacctgc gagtattttg
    49741 gctttgcttc cattttttcg ttttgctgct acaatcttct gatgtagatc tctgctgtaa
    49801 aactcgtcat aagacatttg tttcctaccg tatgtaaaag tcatagttac cggtcccatg
    49861 ccaaggggat ccatttcata tgtcccgacc acttctgttt ctggaccaat aaatttttca
    49921 atatgatctg gatgtgcgac tacaacttga tctcgggtat aaccatccct caccaaagca
    49981 gcttcaactt tccgtagacc ataaggagca taattagcac gtccatctgt ttgctctata
    50041 ggattgcaga taaagtcaaa tagtaccttc ggagtaacct gctgtcctag gacacgatac
    50101 cagaacgtgt tatgatttct attcggatcg agcgctggcg cacaaccaaa aaacgatgca
    50161 agagagattc ccctgtacgg agacattaga gtacggtctg cactaagtac tactttggga
    50221 tatttcataa tctttccttc ttcttcttta atccgccgtt ctctggcata taattatacc
    50281 gatgatttga agtttttcta ctaatttgta gaagggaatc ccttaggata ttctcagtgt
    50341 catcgagctg tctttctagt agtataggtc gaacgagcat tccgatcgtt aataaactgt
    50401 tcaccccact gtgctcaaaa tgctgccatt ttcgtttgtt gagtaggttt aaggcattta
    50461 gtgcacgctg tttgtgtatt ttcgactact caatatctct ctttaatact ctcccttaag
    50521 aaaacacgtc attgcaggat atcttcaagg agatgagact ctaactgcaa tatttgacct
    50581 tcaacaggaa aattatcctt aatctaatct ggttaaaaat gctccaatag tctcgaatta
    50641 gggcgatgta gatctaaact tgatcgcctc ttcaattagg tgttggtgca tgccagggga
    50701 gacacgtttg caagtgtaaa gagaatccca ctagctcagc aagcgttaag aaattgttaa
    50761 gatcgttgat gccctattaa gggaagagag taacattaat gtcagtaagg aagaatattt
    50821 atatcattta attttacctt ttgtcgaaaa tgaaatgtca aacagggcat ccgtaagcaa
    50881 ttatgaagat tcaaacccga acctcaggtc gtcgggctct caaccgccag tattcgtact
    50941 ctgtgaatca tgtcattggt gtgcaacata tctgtataaa gctagattac ctttagataa
    51001 tgagtgtcct caatgttcta gtgatatgtt gtcgagcttt cccatattat ctaccgaagc
    51061 cttcacattc gattacagcg aaaaacgtgg aatagagtta gaatttaggc gtagagataa
    51121 gaagatgctg atcagctagt caactggaaa taattcgatt cgcgacttat agatgcccac
    51181 tctccgtttc atgattatct catgtattcc tatattgtcc ctcgagctcg gaatggatga
    51241 agaatgagac gaaatcagaa acatcccgaa tcgccttatg tagtggcgtt actctgggtt
    51301 actaaaccaa tctagatggg acggctaagg tacctctcac gtttttttac gttttggaaa
    51361 ttggaatgtg cccctttctg taattcctcc tatatagcat gtggctgtga taactgccgt
    51421 gatacagtga tcgaacgaat agcctacggg attgacgacc cactcctggt gatttctttt
    51481 acttatctag cactcttgtt ttcatccact agacgattct aactgagggg ttaactagtt
    51541 ctaactagtt taattaaaag attatcttat gtagatgcta actacactgt agatgacaga
    51601 tggtagacaa agaaaaaata aagtgtgatt tctgtgggaa atctttcaat tccagaaaac
    51661 agttggaaca gcacaagcag gacgttcaca gtgcggcgaa taagaataac gtaatcatac
    51721 ccggaaagaa atctttcaag ccctctaaga aggtgattgc tacgattggc gttggcattt
    51781 tagtcgttat tatagtcagt ataggggtgt tttctgccac ggcaccgcat gtcccgtctg
    51841 ccttaacaat aggtggtata caatgtagtt cgtttgagca acttgttttt catatacact
    51901 cccatcttga tatctttgtt aacggtaagc ctttcacgat ccctgcgcag atcggtatta
    51961 tacctggcaa atgcttctat tggttgcata ctcatgatga atctggagtt attcacatag
    52021 aatcaccagt aagtagaaac tatacgctcg gacagttttt cgatatatgg aatcagaaat
    52081 tcaataacac tcagctgttt gataatgcgg ctaacggtaa caatacgtta acagtgtatg
    52141 taaacggcag caaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
    52321 gtatgtaaac ggcagcaagg taccaagtgg gacaaattac agagacatca aactaggcgc
    52381 acatgatgag attgcaatag tttatggtac tcctccaagt actatacctt cgagatatat
    52441 ctttgctgag gggttgtaat attggtagtc acttcttacc accctaccaa ctttcccgac
    52501 gctcccataa aatgtgaaaa ttgcagttac acctttagag cctgacagcc cacagagccg
    52561 taaaatcatt catctaaggc agttcggata aaacttttac atcacgatga gttggacatt
    52621 gtccatattc ttgttagcct gaaatttttt ggtcatttct ttcacctcgc gtgcatcacc
    52681 tctaagaagg aatatttcaa ggcatctgtc tctatcaatc ttgctatgca gatgcgtatt
    52741 gattaattta tcgtagacgt gtctcatttc cgtaacttga tcatcagaat cctcgtcatg
    52801 aatcgctaga aatagtgcat gaagcatacc agtcaagtcc tgtctttttc tttcttctga
    52861 tagtaagtta cgaatgccag ctctcactat ttccgacctg cctgagaatc catgcgattt
    52921 ttctaatttg tccatctcat ctaaaatagt tctattaagt gacacactca ctatagtcaa
    52981 cgttgttgaa tgttattaag tatcttatat acctattcat agaaattaat aacaaattta
    53041 aatatattta taattgcgtt catgtcagga tgggctatgt ctgcgggtca aaagctagca
    53101 attggttgaa tgacatctct ttaaaaagag ccaataatgt aggaacgctt tagaataagg
    53161 atttttatat ggtatgttta aattactaat atctcgaaaa ctgtaaaatg acatccatta
    53221 tctgctctgt cgtattgata gtaataatcc ttgcaactag tattcttgtt tcaattctaa
    53281 acgaaatcga taccgcttca ccaacaaaca ccgcggtagc agcagtagta ccagtaatat
    53341 ccaaaaataa caataaagtt actgtagata aatttggtat aagagaaatt tatcccacca
    53401 aatcaaatgg aggaaaggaa tggtttatca atatgagttc tccattaact gacaaaagct
    53461 tctctctctc cggtggcgcc gaaaaaacaa attcttcttt agaaaatgcc acctcatcaa
    53521 acggtcaaat aataaaacag cctgatggtt catatcaagt atacggtgtt cggaaaactg
    53581 gcagattcga cttttctgtg cgaatgagtg ttaatgcgtc agatagtgct aaatggtgga
    53641 agaatataga aatgacaggg tatgttaaag ttgtatctga tacttctagc agcgctgcat
    53701 tagattggta tgccagaggt cgtctacata ttagcagcag tccttgtgaa ggtattgctt
    53761 atcatgctgg tttaagagct gacggaagtg tatactggca aaaagagata tggcatactg
    53821 gtggatacac agattttagg agtaatatta ctgccacccc ttcacttctt ggaagatggg
    53881 tcggtcttaa ggtaatcatg tttaatataa acaatgatag cgctgtgaga ttgcagactt
    53941 atctggatga taatgctact aaccattgga aaaaagttgc agatgttgta gataatggtg
    54001 ggtggtatgc gaatgctcca aatgaccgat tttacagtgc caactgtgga agatctaaag
    54061 attatatagt aacaaacgct ggccccatag ccacatttag atcggataat atgatatggg
    54121 actttaaaga cttgaacata agagaaatac aacctccgtc ctgacgttaa ggtcgatact
    54181 gtaaagcaac ttacaactaa cactagctca acttataatc catacgtatt tggcaatgca
    54241 actcaagaat ggatagaaaa agaacacagt gtcaagatct tgtttagtac ttcaccagaa
    54301 tatccttcag taggaaatct tacacaactt agtttcaatg tgcaagattt gaaaacaggt
    54361 agccgcctaa agaatgtaaa cactattgtt acagtaataa ataattttac tgccaatgtt
    54421 ggtactggta aaaataaagg aacccctaac ggagacttct tcactatttt aagaatatat
    54481 cagtgcctaa cgggactttc tctgtaacgg accattttct gcaagcagga acgcatcaaa
    54541 tcattgcaag aataaactca aagaataata tattttgcgt tctggcatct tttaatgtca
    54601 tagtgtcgtc accacattaa ttatgatggt agataccaca attcaatatc gtagttttca
    54661 gaaaatggtg tatgtgaaac tttgctagct tttcacccta ttctgttggc gtagaatctc
    54721 cagccaatct tattaatagt gctcaatatt tggttttcat ttattgcaag gaattaacta
    54781 ccccacttct ttcatcccgc tttcacctgt gatatgataa tgaattcgta attctttata
    54841 gcactagtca ttcaaatttt tcggactttt ggtggcatgt ctttgtttac accggtgaac
    54901 tccgcaaact tctccgtttt gatctatcaa tccatctttt tgagcgatca tgaccgagat
    54961 tcatatttag aatatgctgc aaattattat ttatggttgg atagattgga atatgtggat
    55021 tatttaaaat ctttaagttc gagtaaaata ttgacccaac gtaattattg atgtggataa
    55081 tttttttgag cctgttctaa tcaaaggcga gagatgccag aataggtaaa agttatttgg
    55141 aactgcacac gtttccccaa atcgttagat gaagacgtta tattggatga accaattgtt
    55201 agaccttgta gcggttgtaa tgttccgatg cgccagccac atagagcctt atcgttgatt
    55261 ggacggaagg agggcacagc acaaagtcat aaataaaatg ttcacacaat tttttgaaaa
    55321 ttctgaattt tgttcgataa gtctgaccaa tatgaggtgg ccatatcttg actcagccat
    55381 atcttgttta gatatatcat gttctggata tttttcgttt tcacaaaaac gtcaccgatt
    55441 ggatttgaag tcttcatcat gattatgctg tctaggggaa atagattgat ctactaccct
    55501 tcttttcctt gttgcaaaac catatccgaa agcgccagca ataattatta ttgctaaaac
    55561 tggacctgtt aagggcggta ctatttttac attggttatt actgcatacg attcgaaagc
    55621 tgcaattgtc agggcgactc cgctcaacga gtatgacaga tacgtgaagc ctttggtatt
    55681 aaacattcta gaaaatgctg agcgaagaac tacgctatct aaagtatcaa caggaatcaa
    55741 acccaaagca aaaaatccag caagcatgag tgctacttgt actccaagcg ttgcggaggc
    55801 gaccactgat aacgttaacg ctgatatctg ggtagcagta tcaaatccta taccaaagac
    55861 taatccagtc acaacggctg aaccaaaggg tcttaaaatg tgagtcctag taactatttt
    55921 gctagcaaga atagctgatc cagttttccc ccatctcttc attgaataga tattaattgc
    55981 tccaattgtc gccaaagcta tagctcctgt cacacctcct gagaatgaaa gctggtctag
    56041 tttgctactc gttgtactgc ctactatgta aattatcaga agcatttcac ttaatattga
    56101 tatcatgtga cctgcactga aaccagtacc tacccatcga gcactttttc ttgcattgtg
    56161 catcctaaca agattatcta tggcagtaat atgatcaaca tcaagtgcat gacgcattcc
    56221 aaacagaaac attgaaggaa ttgatatggc aacaaatgcc tttaggtcgg ttaccattat
    56281 tgttcaatgg tcctactgat ctgtttcaaa ttgcctcttt catccttacg tattatttaa
    56341 acaataggat agtttatcca acgcactgct agtataagta gttatcaaat aaagattaaa
    56401 tgctagaggt gaatgaataa gtacgtaaaa atgcactagt gaagtaaggt atgcacaaat
    56461 atgttagaac attaaccaaa attgcatcta gatatgctcc aagtagaatc ctttcttttg
    56521 attcaaccca tgtgattaaa gtattacagc tcttagagga ggaaggacat gtgagtagaa
    56581 acttattgtg tcaagaatta tcgctgggcg aaggttctat caaaacatta gtaaaacatc
    56641 taaaaatgga gggtttgata gaagctacaa attatggtac tacgatgact gaaaaaggaa
    56701 ggaaacttgc ctcagaaatc ctgtccttga ttccggcaga gacttctatt ccaaaatgct
    56761 ctattgcatt gggtaagttc aattatgcag ttttggtaaa agggtttagc tttgccgtca
    56821 aactaggaat agaacagagg gacggtgcga taagaatagg agcgttggga gctacaactc
    56881 tgttgtttaa aggtggtaaa tttatgatgc ctggtatgac aaatcatgat tcgctcaaaa
    56941 atgaatccgg tatatacaga atattaactg atgaattgaa accagaacaa gacgacgtca
    57001 taataatagg aagtgacaat gaagatagaa gaagagcaga ccttgccgcg aaaaatgccg
    57061 ctctaataac aataatgaat catgatagac atgatcatat ctgatgatat atcaagctaa
    57121 tatcaggcgt ttgttcgaac gtcattgtct ccaagcactc tcgtgatcct aacatccagg
    57181 cctgtcaggc tacgtgtctc aaaaatatta tttagcggcc acgccaggtt tcatccccac
    57241 ccagtacaaa ttctcttggt catctaaaac ataacttgga accgaggccg cgatctattc
    57301 cgagttcgca cctctgcgtt gaaaccattc aggtgtttca cctggcagcc cgaatccatc
    57361 caaatgcgaa atggcgtagt tattggtgtt gacttgatat tccagctgct aatactactc
    57421 tgtcatttcg gatctacaga acctcagatt aaaccgtagg aaatggggct ttgttggtca
    57481 ttatgccaat tatactactc cttatacaag tactcgccta ccttataatt ggctttataa
    57541 gtttagcaat tactggatat tggatatatc aaaggctaca tcaactggta ttagggctac
    57601 ggtctaataa cttagcgaga cagaggtgca ttcatcgcag aagtaatatt tctgtcctgc
    57661 gaacccgtaa taaaaagtta catattaaaa cttctataat ctaattttga tgttaataaa
    57721 attgtggcat atttctcgag tatttattgg tcataaataa caagttaata atagttatta
    57781 attgatgcgg agatgtataa tgcgtttgga aaaaggttaa taacatttaa taataacaac
    57841 tgagataaat tgttacacat gtcatcaaca gatcattgta cgtgtaatca cactgaagaa
    57901 cagtgcaaac aaaataattg cacttgttgc ggccactgac gtctgttcga agcaccaata
    57961 tttggtgcta aagaatagat atcttcttga aaacatgtat taatctacgt aaggcgactg
    58021 gttatgctct atgaattgtt agtccggtac ctcgagacta agatccctga ggttcccgtt
    58081 attgattagt tattatttaa tttgtagact atctattggt attaataaaa tagttatata
    58141 ggtggtgtga ttgttaataa caaaagttta tatgttaata acaaggtgag ttgaaattag
    58201 gaatcaaatt ttgaaagctt cgttagctct tctgactcgc gttctcttaa ttgcaatgac
    58261 aacaacaata gctataacac ctttgaaagc ggctggtaca aattcgactt ctaataacaa
    58321 agtaacagca aatcagaccg ctaatactac ttcggctaat ccaatcataa gttcaaaaaa
    58381 catcacccct acaatgaacg caggccagca gcctacacaa gctaaaatta atgtcgttgc
    58441 atcattcttt ccaatttatg aatttgttaa ggcagtcgga ggagacagga ttgatgcgtc
    58501 cgttcttgta cctattagtg cagaaccaca taattttgat ccgacaatac aagagatcca
    58561 gaaagcaaac tcagcgaatc tattgatata taatggtgct tctatggaag aaccttggat
    58621 acacaacctc accccacaga atgcaattga tacaagtaaa ggaataaatc tattagcaaa
    58681 tccaaatgat cctgacataa aaggaccaaa cgatccacac atttggctag acccggtact
    58741 agccattcag caagttcaaa acattcgaaa tggtttagag aaggtagatc ccaagaatac
    58801 cgcatattat aatcagaacg ctcaaaattt tataggaaaa ctgaataagg tcgatactgc
    58861 atttagaggc aacctctcta gctccaactg tccaaaaaga gacttcatac catttcatct
    58921 cgcttttggt tattttgccc cccattacgg tttaacacag catcctatac atgaaggact
    58981 tacaccaagt ggagaagtac ttccacaaaa attagttgaa gtggtgaacc tggcaaagaa
    59041 tttggggcta aaggtaattt ataacgaaga cctgattgat cctagatcag ctcaagctat
    59101 agcagaacag attccaggtg caaaggtgat ggtactcagt cctatagaac gcataaacgc
    59161 cacagaacaa aagacaggca ttggttattt agataagatg tatcaagacc tcgctgcctt
    59221 taaggtgggg ctccaatgca agatctagta gcggtatccg tcaagaacct gacttacacc
    59281 tacaaagtca gttccacatc tttttcttct aatgtattag acaatatttc attcagtgtt
    59341 aacaagggag acttgttagg agttatcggt ccaaacggag ctggaaaaac tactcttttt
    59401 agctgtatgc tcggattgtt taaggactac caaggtgaag taagaatatt cggtcatgac
    59461 attagaaaga ataataatct cgttcttcag aacataggtt acattccaca gcaaaaatca
    59521 atcgaacaag ggtttcctgc tacggtacag gaaattgttt cactaggatt gattggaaaa
    59581 aaaaatggtt caaaagagaa ggtagattct gctctcgaaa tggttgacct ttccggtcag
    59641 aaccatagaa gaataggcga actttcaggt gggcaacagc agagggtact cattgccaag
    59701 gctctggtta gcgaacctaa gttattaatt ttggacgaac ccacaacaag tgttgatgtg
    59761 gagacacaga acaaattcta tacactggta aagaacctaa atctaaaaaa caacctatct
    59821 ataatatggg cttcgcacga tctagatgca gtaaataaaa tagcaaataa ggtaatgtgt
    59881 cttaatagaa gcatgttttt tcacggtgat agtagtgaat tctttgggag caatgaattg
    59941 attcgaatgt attctgaatc gagtatgcaa ttacatatgc gttcgcattc aaatcaaaac
    60001 aagaatctta attcaggcta cagtcaggaa gaggccaatg cgcgtcacga cgaaatgaaa
    60061 gggtagtatc aagccaaaaa tggtttttga aatccttcag tacagtttta tgcaacgtgc
    60121 tcttttatcg ggtgtcgtcg ttgcagtaac ctgctcagta gtaggactgn nnnnnnnnnn
    60181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60361 nnnnnnnnnn nnnnnnnnnn nnntgtataa gagacagcag tagtaggact ctttcttgta
    60421 ttgagaagac aatcattatt tggtgacgcc ctttcccatg cggctttcgg tggaatagct
    60481 gtgggtttgt ttacgaacac gtatccaata tggactgcac tactagtatc agttcttgca
    60541 tctctaggag ttactaagtt acgacaatct acaaaaattc ctcccgacgc cgcggttgcg
    60601 gtaatgttgt cctctggact tgcaatggga atcgtactaa taggtttggc aggaggcttt
    60661 aatcttgact tagaaagctt cctctttgga agtattttgc tgataagtat gcaagaccaa
    60721 ctaatgattt taatgctaag tactgtagta cttttgataa tattcaaact ctataagcag
    60781 cttatgtata tcacatttaa tgaagatcag gcaagagtta gtggaataaa tgtaacaagg
    60841 ctaaactatc tatttatagg tcttgctagt ctagctgtaa tctcatcact tcgcttagtt
    60901 ggagttctgt taatatcgtc attaattgtg attccgaaca ttactgcgat gatgtttgga
    60961 aaagggttta agaaaacagc aatgatatct attataattg cggtttcgtc agtattagga
    61021 ggcatagtaa catcatattt tatgaattta gcgcctggag gaaccatcgt catcttgtcg
    61081 atagctatcc tacttggtag tataggcaca aaatctttac tacagaaaat taaaattaga
    61141 aacctcgcaa atgaaccaca gtcattgccg aaaacaacat gagcagtacg gctagtatct
    61201 taatttagca ttgtgtggac cgattacact tccttctttc caaacgaaaa atggtcgtat
    61261 attaagaaca gtagaaaata atagatggta ttaatatttt ttgcatccct aaagtagttt
    61321 tttgtgatgg gtggaatgca attcacagtc ggggtacaaa catcaaagaa tactggatta
    61381 aaaccgacca taaggatcac aataatgtgc ttgttcctac gctgcccgaa ttaatgcttg
    61441 gaatagtctt gtactacctt agcttcatca aagttaaata aagcgcatgg tagaaatcgt
    61501 cgtctgtagc aaagaacaca cctgaagtct cccttgtctt ttcaatattt aagaatatag
    61561 cctcctgttc gtatattctc gatag
//
LOCUS       DAOR01000122            2930 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_96862, whole genome shotgun sequence.
ACCESSION   DAOR01000122 DAOR01000000
VERSION     DAOR01000122.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_96862"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2306..2500
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          857 a          481 c          525 g          872 t
ORIGIN      
        1 tgttaattac gcaagagatc aattctatat cttgttgttg aaaatggtat caccgatatc
       61 atccaaatat ttttatgatt tggctgtaag aattccaatt gtgatatttt atccgaatcc
      121 atcttattcc ctataatttc tataataatt gatttaccag aatatgttta taacgattat
      181 caaagcacag gcttgattaa ttgtgtgtgc cgttcatata ataatcaatt gaacaaagca
      241 acgtagatta attaaatacg tattaatttg cgaagataca aacaaataaa gatagacttt
      301 acagtttctt agctttggcc attccggtag gtcgtctatg agttttacaa atgtatctgt
      361 gttagaacaa attcgtgcta gagttgctaa aacattaacg gctggcattc agctcaatat
      421 gtcaaccgac gaatctaaaa aattgctaaa gcggcatgta aatagaaaaa caaatgttgt
      481 aattatgatt atagatgtca ataattcgac agaaatgagc ctatctttac cggaggatag
      541 gtttgcttta ttgattcaaa catttgctca agaaataagt atcgctgtga caggttatgg
      601 cggatatgtc ttcaaatatg aaggtgatgc ggttattgca gtttttcctg gagaatatga
      661 tcagccgaaa gcatgcaaca atgcattaaa ttgtacagct tcaatactgc agatcattag
      721 aaaagtaata aaccctgcgt ttaaggaaaa tggtttacct gaaatcaccg taagaatagg
      781 attgacttat ggttatgctt tagtagtgct ttatggaaac agtttagaaa aagctcacat
      841 agacataatt ggttctagca taagcttggc ttcaaagatc gtctctatcg cgaagcctaa
      901 tcaggttttg gttggcgaat ccatttacaa tatcgttctg tcatcagaat ctatgagcaa
      961 tagtaacctt atagagataa atctcgatcc aacaaaatgg aaatatcttt ctcgttctga
     1021 ccctgacagt atgtatcgtg tatacgaata tacagaaaat taatcctacc tctcttagta
     1081 tttgattctg tattagaaat tacgagttag gtatatctag ccagcatgag tgaccctata
     1141 acttgtagta tagcgtgtaa aaagaatatt attaccacat aatgatcatc ggaaggatca
     1201 ttctgctcac taacaatgat catgagaata tgtttcggta gtaatttaga gaagttcttt
     1261 catcctttca agcattgaat ccatcctctc acagctaatc tttggcctat tcttaaacat
     1321 tgaatggact ggtcctgctc cgtcaccaat gacacgtggt ataatatgta tatgaacatg
     1381 taatatttcc tgtcctgctt ctaagccatt atgtattgct atagttgaag ccttgactcc
     1441 agctgcccct tctaccgctg cagttaccac ccttagtaaa tcaaatgtca cagatgaata
     1501 ctccctgttc atatcctgta ctttagaata atgtcttttt ggaatgatca gagtgtggcc
     1561 taaggataag ggaaaagcat ctgggtcttg gatggattat agatactaat ctaggtacag
     1621 aagttataca gcatagcggt ggaatagatg gctatagtag ctttgttgga tttaatccaa
     1681 ccaagcaaga aggagtagta ctattatgca gctgtgaggc taaggatatg ccttcgcccg
     1741 taatccaatc cttgattaaa atgaagttag atacattgtt ttagctgatg ctgttggacc
     1801 cactagaaga atatgaatag ccgagtcaga ttccagagcc atactaaaaa gtgttttgat
     1861 agggtcatag ccaataatat cttcgaacag tcggttattg ttagttttag ttatcttaca
     1921 tagcaacatt ttacaaaagt tcagtttatc tttatctttc atgaatccga tagcaattga
     1981 tgaatcctac tttactagga ctgagccatg catccaggga tgtacctcgc aaaagtaatt
     2041 gtatgtacct gccttcagaa aagtaaccgt aaatgtagtt gacgatcctg gaaggaactg
     2101 ttgtcctttc ggaattaacc agcgagaatt aacatatttt tcgctaccaa tcatactgta
     2161 gtttgcattt ggtggtggct agtgtttcac attaccttgc gaatcaatta cagttggaat
     2221 ataagacctt gcattcatac tactcttgga gcaataactg tagcttctat gaccgattat
     2281 acttatcctt ttaggatcta cttcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccttttagg atctacttca
     2521 agccgtggta taagaatatt caaagctttc ttactttctg aagtggatta catacacttg
     2581 tgtattgcat cccaccgaac ccgcgtcaat attggtgatc ccaaatttag tatgtacaag
     2641 caatggggac aaggggacga gagacttgta cctatttacc cggagtacaa tgacacgata
     2701 ataagatggg ggaaagaaga agtgctagag tatcttttga aaggtaacgg ggacggaaga
     2761 gagtttcttt attagaatat ttccgtctgc gagatctgat tgtacggttt gctctttcaa
     2821 acaaactaga accaaaggta acttctgggt ggaaccaggt ggatagtaga tggaagaagt
     2881 tccactcctc atagcagtat acgacatact attgcgtctg tctcttatac
//
LOCUS       DAOR01000123            9508 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_2652, whole genome shotgun sequence.
ACCESSION   DAOR01000123 DAOR01000000
VERSION     DAOR01000123.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_2652"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3914..4198
                     /estimated_length=285
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2797 a         1773 c         1726 g         2927 t
ORIGIN      
        1 ttttctttga gtgctttgag tatttcagca ctcattccta actcttgaaa tgtagttgtc
       61 aattttaatt tctcgttttt ggatgttaca aaagtcacgg aaaatatgaa aagtaatgtt
      121 cttctatcta cttaacaagt cagccgtaag tcctttgata actatctgca caatacacag
      181 atataaaagt attattatgc agggttttgt caagtcttcg attgatttag aacattttca
      241 cacgttgtca aaaatcggtt gaaaagggtt tagaagagga tgacgcgtat tgtatatatg
      301 agactctatg attgagaaag caagcgaaaa tgccattaat acacatacaa cgaaattgga
      361 aaaccgtgat aatttccgcg gcggtcacat taatagcagc gtcgataatc atattgtatc
      421 aaggccaaat aaacgcgttc ttcggtatga gtggctcagc caatattcta cttgtcatac
      481 tcatatgcgt ggaagggtcg gttccttttt tatccgttgg ttcgaaaaca aagaccgtaa
      541 cagggaagaa aggcttggta gaatcaagaa tcattgcgct acgttaattc agcactataa
      601 atcgttcatg acaacgggtg tatacgaaga atcatcgctt caagacaatt tatccagatt
      661 tccttattgg agatatctta tggagcatct ctatactgga catagaaaaa catatgacga
      721 gcttaacaag gctctaaaga cgaacaggat gttcaaggaa tcgtcgaatg acgagaatta
      781 caattcattc agattataca tggatgaatt gattacagga aatgagcttg caggagcgat
      841 aacactcaaa ggaacgtgcg accactgcat tgattttcac gataagaagg aaatccccca
      901 attgaaaaag gagctatctg agctcatttt ttcttagatt taaatttcat tcttagataa
      961 tcgctcaata ttaccctaat cgtctctggt acggtttcca aatatcgctc attccgttcc
     1021 ttttctagaa cggtgaacat ctcttctgga atagaaatca tcactttctt cattgccatt
     1081 gtctatacca atatatacca ataggtagat acatttataa atgtagcgtg tatatctatt
     1141 ggtatccaat ggtagtcgca gaaaacagaa gacaagaaag aggaagatat ttagctggct
     1201 taagaggatt tgttaataaa attggagata atttgtatag gataaaatct gcatcaggaa
     1261 atggaataga atatgttgtt aagagcgaac aaggaaaatg ggtatgtgaa tgtcctgatt
     1321 cgatttaccg taaacaaaaa tgtaagcata ttttcgcggc cgaatttagc atcaaattaa
     1381 aggaacaggt aaaaaaagaa attattattt cgcccgttaa tattcaagat tgtcttttct
     1441 gtcattcttc atatttgaag aaatttggcg tcagacataa caagagtgga aatattcaac
     1501 gctttatttg cggtagctgt aacaggacat ttagcattaa tttaggcttt gaaaaaatga
     1561 aacataatcc tcaggctgtt actagtgcta ttcaattata ctttagcggc gaatcattgc
     1621 ggaatacgca gaaatcaatt agattgttag gcgttcaagt atcgcatcaa acaatctaca
     1681 attggataga gaagtatgtt catttgatgc aaaaatatgt cgagaaatta aaacccaatg
     1741 taggcgatat ttggagagcc gacgagcttt ggcttaaggt gaaaggagat atgaaatatc
     1801 tatttgcatt gatggacgat gagacgcgtt atttaatcgc gcaagaagtc gcagatacaa
     1861 aattcataca tgaagcgcgc aatttattcc acaaagggaa ggaagccgtt tctaaaacac
     1921 cagcgagatt gattacggat ggattaccag catatcatga cgcattcaat aaagaatttc
     1981 ggacacgaaa aaacccagca gaacatatca gtactatccg attgagtggc gatatgaaca
     2041 ataacaaaat ggagcgtttt aatggtgagg tcagagatag agaaaaggtc atgagaaatt
     2101 taaagaaaaa agatacacct atcctagatg gatatcagat ttatcataat ttcatcaggg
     2161 aacatcaagg attgaaaaat aaaacccccg ctgagatgtg tggcgtgatg attgaagggg
     2221 ataataaatg gaagacgcta atcgagaatg cgagtagaga ctttataacg gatgcttcgt
     2281 aaatataatt gacccgtaga tgacaccaga gaacgaacaa gacttgggca agctacagca
     2341 agtcagaatt catttcagga ctgaagaaga tgacgagaag ggtcttttta cgttgatggt
     2401 ctctgggatg cctgtccatg cattgagaga taataattac gttgtaaaca gaatgcaaat
     2461 caaaatgctt gacgaaagag aaatagaata tgagaaaata gattgacttc tatcccgtct
     2521 caggggaagg tacgtataga aatcatcatt ccgttaagag acaatgatgg aaaattaata
     2581 gaaatcagca aacataaaca aacaaaagat gagataaccc aaagattcgg agcgggcaat
     2641 ttcatgattc cttcggaggg tgcttggacg aaccatagcg agaatgacag aatctatctg
     2701 gacatcaatg ctagtgtgtg gctactcatt gataatacgg agaacaacat taattttttc
     2761 aaaaattaca aagagattct taagaaccga tatgaccaga aagtcatata cataaacatt
     2821 atgccagcta gcgaattata aatagtgtca ccaaccgact ttttacaggt ttatttcaac
     2881 cgaagatttg acagaacctt atgcagtgac ttggtcaata tatttttgat tagcctatgt
     2941 tatgatatcc tgggttcatc gatatttcgc attatcttgc tctttctttc ttcaccttat
     3001 atttctgatc tagtcttcac cccgcgcccg aaagtcaatt gtgaccacta ggacagctcg
     3061 ttccacgggc tttctgtctg tctcaccttc gatggatcag ttcacgtatt ccttttctta
     3121 atcgtcatta aatattgaat cgagggacat attcaaattc gtgaccagac aacgagaatt
     3181 tattccgttc aaattacatg atatatatta cctggttgtt ctgcgtttga ttattactat
     3241 tgagttattt taactgaaga tatagatctt tcagtttgat tgaacgatag taagtgagtc
     3301 ttgaataatt ttattcaagt tagggtctgt taataaaaat gtctacatta gttatcctcg
     3361 tttatttcgg tttatagata cagcatcaat ttcgtcaact caaatatatc tagctgtgat
     3421 tagattcacg aaacgaatat cacacatatt tcaggggcta tcatggccat tttggctttg
     3481 aatttgctat catcctctac ttcagatatt gtacctattc aaaaagctaa ggcaataaca
     3541 cagataaata ctattagtca atattatcta aaacaactga accaacaacg acaagagtgc
     3601 ataggcaatt tcacttgcgt ggacaatttc ttcattgtat taaatcctac taaaaatgac
     3661 acgactataa aaataaccaa tctggagtcc aaaatttaca atactgataa cgttccgttt
     3721 gaattgcctt tcccgtgatc cgttcgtatt tcctttaaat gctactgtag cttgggatat
     3781 tatgcaggct gctctggatg gtgcgataca ccatggaagg gtccactata tccaaccaat
     3841 cttaatccaa gtactcaaga tgcagctgcg ttgggaaata caatttgctg ttcgtccggg
     3901 gataatggat cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     4201 actcaaggcc gcaaaatgga gggctaagcg atattctagc acatgctcac tctcttggtc
     4261 cttatccctt ggcatgagtg caatatcatg atcgcaggac aggaggagaa agaatcacta
     4321 aaaattatgc tattctgttt tattattgtc gtaacgaatg gctttacaac taagagaata
     4381 tgagcctaac tatgtttcaa aaaaaatttg gtcagtaata atatctcccg agctgacgta
     4441 ttgacagtta tccaactgct gattattacg cctgtccatt tattataacg aatcaatgaa
     4501 atctctaaat tcaggattat gaatgtcttt cattgcccat acgtttttga atctgggaat
     4561 ggtgaatatc gtttttgcaa gtgttttcca actagaccaa acctccgtat ctaccagctt
     4621 tgctttgttg agtcgaaata agaattcaat caaatcatac tcttcagggt attgactagt
     4681 gtaaagcgtt ctgtaaagat tgggctttcc tatgccatgt ttgtaaatgt ttcagtatct
     4741 tccagtctga tccttgcttc cattatcctt gaataaatct cttgttctgt ttacatacgc
     4801 atattctttc ttatcgtagg taattgtagg aatgatatgc taagcagacc tccaagtacc
     4861 atagaataag aaatcaaagg tattatcaca gcagaaatta agattgaata catattatcc
     4921 tctgcgtctt tgtccccttc ttttagtagt ttcgcagtga ttgaaatatc gcattctcaa
     4981 tcagagttat cttcacgtat ctgacttaat attccaactt gtaaacctga cccactgaac
     5041 gtgacaaaat attcggtcat gagttgccac tatattctct aataaccagt aaattcttca
     5101 acctttattc tttcttttgg aatgtgtaaa tcatcctgta acaggttttg cattgctttt
     5161 agcattcctg gaggaccaca gacataaaat accgaattat taagttcact tgtagtcaaa
     5221 aacttagtta gtatggtttt gtttatgatg ccccgttctc ctttccaact gctcgaagaa
     5281 ggtgcttgtc cttgttcttc tgctgctgta atagtgtaaa tgatctttaa attcctgttt
     5341 gtgttcaagc attcatcgaa ttcatttttg tacagtatat tagcctgatc tctattagaa
     5401 tcaaacatat ttatctttaa aggcagctgc ttatctgttg catactttat catacttcta
     5461 aaaggagtga cacctattcc tcctgataga agtactgcag cttttgaata atcttcgtgt
     5521 aacacaaatt ttccctctgg tcctcttacc ttgactttag ctccttcttc taatgatgaa
     5581 agtctctttt tgtaaggtgt gtccctgatt cgagtactaa tcataataaa gtcctcagtt
     5641 ggcgaagacg caatagtaaa atgcctaatt ggtcccttag gatcattact aactccaccg
     5701 atatcgaaaa aggcgaactg tcctgcggta taatctaaat atatcttatt atcgctattt
     5761 ggtcctcctt gttgctcatt ttgcctgcta aactttaacg acataatatc agtaccttca
     5821 aacttttgtt tctctagtaa tgttagctcg taaacaacag aggctccctt tcctctagtc
     5881 gtcgcctctg atgtaatctc gtgttgcgtt agttgtgctt ctggtttttt cctaactaat
     5941 atgtcattat attctacttt tacttcatag actaattgtg gtgtttctgc aggaggattt
     6001 ttcacttccc ctgtcctaac gtcaaatttg gaaccatgcc atgggcattc aacctcgtaa
     6061 ccctcaagcg taccttcttc tagaggacca ccctcgtgtg aacaaactgc atctattgcg
     6121 tatactttgc ctttaaccat cgacaaaacc acgggctttc caccaggttc aactttcaga
     6181 agaccacctt cttgtaaatc ttgtttgttt gctacctttt catatccttc ttcatctttc
     6241 attttattca ccttcgatta ataccacgta caatattatt aatccaactg gttagccata
     6301 gatattcttt tgctggtcac ggtcttatta tttttaagca ttggtgtaac tccagatgac
     6361 acggaactag atgaagacga gaaaagatta agtaatctcc tacccagctt tcgtactcgt
     6421 actgtaaata gcattagtag aagacgtggg tgccaaagat cgtggagatt ttgtcctgtc
     6481 gttattatcc cgtctgccaa ctcatcggac atcctcattt cttcctggct gtacgctcgt
     6541 gtaacttgct actattagtt gctattggga tgtgttttct tcaaatagtt gttctccttg
     6601 attgagctga ttggctatct gatatgttct gccttgggaa cgcatggtag gggaatgagc
     6661 agctaaatat ctagatgctg ccagcaaaat atttactttt gcagtattat tgccgtcatt
     6721 attatcatta tctagaagag aatactgcct gaaagccgct tccatcatct gaatagaatg
     6781 aaagttacgg tcttctcgca gcaaaagctt tccaagcata gacgtaagtt gatctgatct
     6841 tccgccgcga taaagataat ctgctaccag ctgtcctgtc tcatttactc tctgttgttt
     6901 gtccaatagt tctgctaact ctcttagtat ggtttcagga tcattagctt tatcttttgt
     6961 cgatttctga ggtattgtag ctggaggtat gttgagaaag cgattaagat atatcctcat
     7021 tgcagcatcg aacactcctc tcaatagctc cattgatggt attcgtttta agccttgatg
     7081 tactgcattt gaaaatgtaa atgtgtgtaa tgcggcgtcc caatcaccaa attcattgcg
     7141 tgtatgaaat tgtgctattc ttaaggctga agcatgacac acgatacttg ccaattcctc
     7201 ttcagcagta ccttgtctta atgcgtctaa taatgcatta acgactgatt gtggattatc
     7261 gcctagtaac acttctacta atgcatctct atcgcgaccg ttaatgctcc atgtttcttt
     7321 tggtttctca tgtttgtgat tattcttact atcaattatt gctctttgat gtgctctttt
     7381 ttctctcatc ctctttccgt tttctaaaac tctgggtaat tcttgaaagc cactttcaag
     7441 aatagtaact aaatctattg gataacgcca tgaacttgat tcttccatcc tttcagccgt
     7501 ggcataacca ggtacaaggc tacctaatac agcttctgcc aatgccttat tgttttccca
     7561 tccaagttca tcaagggcct ctaatgcttt attagtaaaa tcaagagtat gtccaacgtc
     7621 gaggaaacga tgatctgtgg cggctgcaaa aagcatatct gccatctgtt ggtcattagc
     7681 accaagtctt agagcggtag taacacacct ctccgcagct tgggcatcac gagattcaat
     7741 aaactgccta aaccaacgct taagcgtacc caagtcaggc caaggctctg ggagaggcga
     7801 aactccaaac ctcggcgcca tggaagcaca gtcttgcgca acagcgcaaa gaccatgata
     7861 aagcgcatgt ggtctatcct cgctatcaag atacggcatg atgttcatca tgcaggtaag
     7921 cattgttaat ccttgtcccc atccagcttg tttgtgatga gtaccaaaat caagaccaat
     7981 acagaatggc tttactaagt cagagtcatt actatcatct ccagattgat attcttctcg
     8041 tattgcgatg actgctttag caataattaa ttgaatattt tgttttagtc catttcgtaa
     8101 cagcatttga taataagtat tatcgtttct agaatacaaa gcggaaatat ccacccaaac
     8161 ttcagtttca tttcgaatct ctacaggata agaacgcaca tcaccagccc attgatcaaa
     8221 tgttcctcca ctattgagat caaagcgagc gtgatgccaa tgacatgtaa ggataccatc
     8281 cttaaccgta cctagattta gtggaaaacc catatgggga catctattat ctactgcata
     8341 gactctgaaa ttatgataga aaagtgcgat tgtactattt cgtatttgta ttgataagca
     8401 tccaccagta gcttctcgta tatttgacag atttgcaaca aatatgtaat ggttgtcatg
     8461 atctttcgag atagttgcca ttagcatcaa tcactaagat agatattgta atacaaacat
     8521 aaagtgtttt gtcgttcttt ttccagactt ggaatcggcc ttcctcccgg gagaaatgat
     8581 ttctatgggc atcagaatgc ccgctcttgg gtgttataga agaatctaca gcggtgcaat
     8641 gtgtgctgta tactgatgat gggcggtgtg ctgacgcttg gtagttaaaa taggttatta
     8701 ttttcggttc tgttaacata tcgactggtt tgtaaagtgt taaccgtcgt cggacaaaga
     8761 gctataggca agcccgttat ttttgttatc gttgactaca ctagaagaaa caaagaaaat
     8821 tgcggaggaa tatataaaaa aggaaattga gatgttatac aacctatcgt tactaagcat
     8881 cgaagactcg acgcaattca aatcatggtt tacagcacat ccgcattatc agcgtctacg
     8941 aggcttttcg aaccaaatca tcgccaacaa atggaattta tagagaagta tagaaaggtg
     9001 gttattgtcg caaagtgatc cattagatga tttctggaaa caatatgaaa aaacatctgc
     9061 tgacttggcc gttatttggc gagacgttgt cagaaacaat tacggattat tgttaaatat
     9121 tattcctctt ctgactgaag gtgcgaatat tcttttgaga aaatatatag ccgacgagta
     9181 caagaaaagt agagaatcta cagcatcatg attcgtcaat caagtcctga gtggttctta
     9241 atattatttc tatcccttgg tcttgtttga cccttgccac ttcttcataa gctcctctgc
     9301 caaaggaaaa cgcgactatt acaccctttt tctttccatg tctctataga gcagtctcaa
     9361 aattatcaat aacgtttctg ccgacactga tatacacgta gattgttcat atatcatcat
     9421 gggtaaatgg aaaaaggacg ccaaagaatt tgtcgtcggc gttaattatt ttgaggatag
     9481 aggctatcag agcgcgattc ctaggcct
//
LOCUS       DAOR01000124            4965 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_107509, whole genome shotgun sequence.
ACCESSION   DAOR01000124 DAOR01000000
VERSION     DAOR01000124.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_107509"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2089..2390
                     /estimated_length=302
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1532 a          841 c          860 g         1430 t
ORIGIN      
        1 ggatatacac ttcctagcag aagtgtaacc taagtctgta agtgaatttg ttacctatgt
       61 gtgtaagtac aacaactagg taatgcatcg tccgtgctat ggtatctttt gtctatatat
      121 accaagccaa ccttagatat ctattatgca attttgccta gaaatatggg gtacagatta
      181 tgaaaaaatc aaagagactt gcatattagc tgagaaatta ggatattacg gattctacta
      241 cggagaatca ttagcagaga ttgatttaga ttgctggagt ataatatcta acttatcagc
      301 tataactact cggataaaac tagggcctgt catcacttac ctaattcctc aatatagaaa
      361 cattgcacta cttgctaagc aggctacgac attacaagat atgtcaaaag gaagattgga
      421 atttaggact ggtgcaggag caacacatga ttacgcagta cagtggtggt atccatatgg
      481 aatagattac cctaatagag cggatcgagt ttcattactt gatgaaggta taaaagttct
      541 caaatcgttg tggagtaaac caaacgttaa ctttagcgga aagtatttta aaattaatgg
      601 cgccagccta aaaaacccaa aagaaagtat tccaattacg gttgcagcaa aggggaagag
      661 aatgatgaaa atagcagcca aacatgccga tatatgggaa tcttcttatc tttcgtctgg
      721 acattttgca tctcttaaat tgaaatttga agaaatatct tctgaaaaat tcaataatgc
      781 aagcaaaatt atgaaatcaa ttgaattaga tgttataatt gccaattctg attcagacct
      841 agaatataag aaaagactat ttgcaatgga aagagggcca gctatgctta atcaactgct
      901 cagacattct ctagttggaa aacctgaaga tatagcacaa aaagttagag aatatataga
      961 tgttggagta gagcaattct ttctggcatt tcaagatcct tttgattata attcacttaa
     1021 tttatttatg gatacagtga aagggtttac tggaaacaac tcaaactaac ttatcatcag
     1081 agaagatcat atagaatact aacttatatc tgcgcatttt catgcaataa tgtgaataaa
     1141 atagccgtaa agccaacaaa acccgctgcc gccactacca aaaccatcaa agacattgct
     1201 cttcttgatg atgatcaaat caaaagaaag atagagaatg ctacagaagg attaccatct
     1261 caatgtttaa actatctatg taacagagtg ttgccggcat ctacggagat atagaaatta
     1321 tatgtttgaa tttattgtat cgtataggcc acccaaggtt taatctgtta gatttactta
     1381 tttttacaaa tatgtttcct ggaagttgga aagacgtaga acccatagcg tttgcgtatg
     1441 gctaggacaa tacctacaat cagcaaatat cttttctcat gggcgataat attgcagctt
     1501 ctttgaattt agaaataagc cgttttggac cagaattttt gaacgacata ttccgaataa
     1561 ccatgacacg cgctagaacg tgtgtcataa aatcacctac cgaatggatg gattttgtta
     1621 acacctgcta ttctcttgat atctcaggag acgaaattaa aaggaagaaa ggatggcgag
     1681 ctatgaatag tgagaaacaa tcattcaagg tttggatgga tatagataat attcgcaaca
     1741 aagcagttga tgataaatat gatcctacca ttaggaatta cttcagatat aatttaagag
     1801 aagcaagtag agataattgc gtaataacag tgttcgatga gataaagcaa aaaaggcttc
     1861 ttgttgatgg tatccataga tcagctgcgc taaccatggc ttgaagaaga cttaaaaatg
     1921 gacatagtaa ccgtaagaat ttttgagagc tatggctcac atgttgatgt tatttttcca
     1981 tgtgacattc cccaattgtc atcatgaaaa gccaaccctc cttgccagag aattttgaag
     2041 atgctatcct taagtccgat tcagcaactc ttaaaagttc cacaacaann nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctgatattg
     2401 aaaataatgg acgatgatgt tttaatcaca tcataggact tccaaggaag gacggtatag
     2461 aaaagcctat atttgattat gagaaaatcc tgtataacac tatgatagaa tctatactag
     2521 agaagagata gacaaggcca aaacatttga taatagtaac tcctacgaaa catgatctcc
     2581 catcatggcc tacgcattat actaagaatt aatttcatac tcctgtatgt caattctgaa
     2641 gtagactaaa gtatttctag ccaccgagat tacataaaga aagacaaatg caacgtggat
     2701 atctaatctt gattgtgggt ggggctcttc tagcctcagg tattgctatt tcagcaatat
     2761 gggctggctc tttgggtggc agaatcatcc atcaaaatac tattctaaat ggtgtgtcca
     2821 tttatccttc tgggacagcg agtgcaatta cccaagtcaa tgatgtgagc cgccccttat
     2881 cgttggtaat tagtatagag ccgaatcata ataccgatct aagagaaacc gtcagaaacg
     2941 caaatggaga ggtaatctca accaatgagt tcacaaaaca attcttgact acaatcaaac
     3001 cagatgttat aggaaagtat actctcacta tcgataacat aggaggtagt cttgtcagtg
     3061 ttggtatatt gtttggagat cttccgttcg taggtcaaaa caaccaactt aacctaaatt
     3121 tatttggagg cctcgtaaca ggaggaattc tagccgttgc tggaatcatt gctctgatag
     3181 cagggcttgt agtattgata gtggatagac ggagaattac aagaaacaaa acgactagca
     3241 cgtgaaaaga attctgatta tagatgacga agcagatatt acttttgcct cagaacaggc
     3301 tttaacagac aatggatttg aacaagttga tacattcaat gatcctcaat tagctttgga
     3361 gctttaaagg tctatttttt ctggtgattg aatttgattg aatagaatag tattattaga
     3421 atacaagatg gaagttggcc aaaccgacta ttgaaaagag atgaaatact atgcaggctt
     3481 ttatttaaac ctggaaaaat atcgtgttta agccgcttgt ttcatttttt gtgctttgag
     3541 atttgttctg acaaccaact tatcctttta tagttaccgc ctttgagaag gcaacgtctt
     3601 tacctgataa attatccaga ctagcttctc taagaaatca ctctgaaatc tttcaagaaa
     3661 cagctaatta ttggaggtga ctattggttt attcgctcgc gaacatcgac gaaagatata
     3721 gaatggcctc aaactgaagg catacctgct actcttccgc cgacggtaat taaacatcac
     3781 tatgcacctc ttgccttgat acaatatgat aacgatacaa ccacaaaact actttctgat
     3841 tgtcgtcagt acgttcctca cgagtcatgt tcgtgcccac agtggcgtcc tgtcggccgt
     3901 cgttgaagga ggagggcaat ttaccacaat ccttcaggaa caccaccagc gattatcgta
     3961 ggcgaaacag aggctgcaca agccgacaac gaagatatcc cggactctaa atttgtcaac
     4021 gatatataag ggtctactct tcaacactgc gaccgctgct attttttatc atgaaacaaa
     4081 gagcagcttt gtacatagaa aaacaccttt tagattaata tccattgttt tatctcattc
     4141 gtctatcgat tgactttttg acgaatacta ttccagcatt attgactaaa atgttaatga
     4201 atttgaattt gtcaattgtt gatttgataa gatgatctac ttctgaggat atgctagcct
     4261 attctccttg aactacctgt cacaacagta actctttgat attctttatc tttctgcaga
     4321 ctcattgtgt gtaccttttt catctgcttg tgtttgatcc ttgtttgtaa gtggaatgat
     4381 gaagtctttc atattggatt ttaaataaag cgatcactgc agctatctct cgttcttttg
     4441 cttcttctcc tcctttaatc caataactaa cccatcctga ttgtggcata acgtggtgag
     4501 gtgaaacacg tccagaattg attagttcat ttctgatctg catcggatat ggtaaatctg
     4561 ctaaatgatc accacgaatg tgtcccattt ctcttttgtt aatccgaaat tctatacctc
     4621 caaatctatg tggttcagct gtgacataag gccaactcaa tagcgctttc ttaatacttt
     4681 ccaaaattgt acccataggt aatattttat tactctatat atataaactg ccacgtttac
     4741 aaattataca aattatctac agatgtatac tatcacaagg aaaaatagag caactgctga
     4801 tgtatagtac tcccatattt ctgcttgtgg ttttgtacta ttgttatcgt cacactcatt
     4861 ggttaatata gttgttctat gaacaccaac aacaataggt cgtttttctt tttaagtatc
     4921 cacagatgtg ataattatat tagtgatctc ttgcgtaatt aacag
//
LOCUS       DAOR01000125           13368 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_33240, whole genome shotgun sequence.
ACCESSION   DAOR01000125 DAOR01000000
VERSION     DAOR01000125.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13368)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13368
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_33240"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3539..3742
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4048 a         2531 c         2490 g         4095 t
ORIGIN      
        1 aatctaatgc ttcataaaga tattacatct gcacctccag ctcgctttaa tgcgtctcca
       61 ttacttacta ccatatcagc tccaagtttt cttgctaatt cttctttatc ttgtgagtgc
      121 gttataccaa tggtgtaaaa accggcggcc ttggagaact gaattgctaa gtgccctaac
      181 cctccaattc ctaatactgc tatcttctcg tgaggttttg gatcagcgaa ccttaagcca
      241 ctccaaacag tataaccggc acaaaataca ggagctgcct gctcatatga tattccattt
      301 ggaatgagag ttgtcgcatc agcataggct accatatatt ctgcatggct gccaggagtg
      361 ttgataccag tacctatctg ttgtagacag aacattggct tcccacgttg acaccattca
      421 catctacggc atgaggcttg taaccaaggt actcctactc tatctccaat cttgcgacta
      481 gcgacaccac ttcccaattc gacgatttca cctgctggtt catgacctat agtacgagga
      541 aaagccgaag taggcaaatg accttctgtg atatgaacat cagaatagca caaaccactc
      601 gcatgtattt ttattagaac ctgattagtg gctggatgtg gagttggaac atctttaact
      661 tcccatttac catgaattgc tggaactacg gcagctcgca ttatgaaaaa ctactcctcc
      721 attcttttgc cacatgaaaa attatcgatc cccatgagat atggatataa cgtcggtgta
      781 taaaaacatg aagtgatatt ataatgtgat cagtcagatg tactaggtgg gccacttaac
      841 ggcccaccaa agccttctat tccgaattca tttgatgata ccttgatttc gttgtctaga
      901 acaatctaga acacttatga tcattcaatg ccttccttaa cattggaact caaaggatat
      961 ggttcttatt catgctagta gagatataac agcgtcacta gatagcgtat gggatgttat
     1021 agcagatatt gatagggaac ctgagttgtg gcattcatga tacatttgca aataagaagt
     1081 gggcagcata gaagacaata aacccccaca ttttctgatt ttaaggttga tcgcataatt
     1141 gcttcgagga agactatcat aagtaaaaaa aggtcggtgc cttgagaatt gtgaacttgt
     1201 gcccagtcta ctccatatcc ataccaccgc cgcccacccc tgtgtcagca gagttaccgt
     1261 tacttacgcc tgttggccca gtagtgttag tggcagcgca tacattagga ttcttaggcg
     1321 agcagaacac cgcatgtgcc gactggagtg tcaagtttat agcaactgtt gccgtaatca
     1381 gggctgtagt aacaacaatt atagtcttat tggtagtttg ttttccatca ttgtcatcac
     1441 tcattgtgct gcgaagacta aaatcaattc gaacgaaaaa ttctatctat tttcctaatg
     1501 catagaagat taggaggtct ctagtatttc catggaatct gtgctcactt ccagcttgaa
     1561 caaaaatgga tgttccttgt ttgataggat gatttttgta gtttaaattg ataaagccgt
     1621 taccttctat aacatagtaa acctcgtctg cagaatgtgg ttcttgtgta tcaatctcac
     1681 caggatgtaa tctcatgatg cccgcctgaa taccttttgt agttacaaaa tctacaaagt
     1741 agccaccacc cttttcttca agttcagaaa gaagatcttt tagttcagat gggtttttca
     1801 ttaaaatatc agaatctaaa acgattataa ttctatatcc ctagtgcctc gaacgagtct
     1861 tattgctccg caacaagaat ataaatcagc gcacgagtag accgttttgc tgcctcccca
     1921 agataacaca acgataacgt ataacgaatc tcacacgaca gtaattcatc cttctagtca
     1981 aaattacata atataattcg tctgttatgt gcggctgttg attatccttt tcgcctcgtt
     2041 ttagccgaat tataccaata gctgtagagt ttgaacttaa aagagtaagg aaatagctat
     2101 ttatattatc aagtttggag aggatttcag aaagtttaaa gatttctact ctattatctc
     2161 gtggcatata agctgtttat atgaaagatt tcttgaaaac tttaggtcat agtaataaca
     2221 gtaaacgaat ggaattgttt tgaagcttgc cgatcagtaa tcatcaaacc atatttgata
     2281 caattgtgtg tccagaatgt aaccgtactc aatcgtctat ctaatctaca taattcgcat
     2341 tcatttttcc agactagttt cagaacacct ttatccttaa ctatgttgtt cgaagcgtta
     2401 caggtgtagg tcttatgagt gtcaaaaaca tgtgtgacaa acggatttag gattaaaaaa
     2461 taaagagtgt ttggttctgc caaatacatt cagccgagcg ttaccatcaa ttaattgtgg
     2521 attaacgtct gatgagttaa tagctaagat gtacacatac atatttatta ttattttttg
     2581 ttatcacaga taacaataag gatgttgtat ggattgttat gcagccttgg tattagaggc
     2641 gaattgatga tcggcactac agctgctgag atattgacgt catactggca ttcaagacat
     2701 aacccacctc ccaaagtata tttttttaat agaagaatac atcatggaga aataggtgca
     2761 ttgttgggat tatcttcgtt gttcttaaga ggtacctcat ttccaactgc gactgccgcg
     2821 gcaattctca caggcattgg aataggactt gttaaggacg actatctgga tattatggat
     2881 tggtttaagc tcacgaagaa gaaaaacgta gatcaaaaac aaacacgaat accaacaacg
     2941 aaacaggaaa aagaacagat caaagtgaga gtaaatgaac gtcatgatac ttgtaagaaa
     3001 ccagaagaaa gacattcggt tattgctgat agtactacca atatagacaa aaaggccatt
     3061 cttgaaccat taagaaaaca tatacgaaac ataatagaca cacaatctca aacaataaaa
     3121 ctgattgaat tacaaataaa agagacacag aaacacctcc ttggttgatt aggtcagccc
     3181 tgcgccaatt acaataaaga tcgccgagac agaggtgtct gcaaatagcc cagccttctg
     3241 cacttagcta ggatatctta actacagtaa attaattaaa tcaaatatat tgatagaatg
     3301 aatgagttaa gggctcaagt cattcagcag cggcttattg atgtaggaag ccaaaatact
     3361 gagtgagtct acaatttaat tacatatttc caaataatat aataagttag tttagtcaac
     3421 gttcttactg tgtgatatat aggcgtactt aaggttgtaa tccctcttaa gccaggttga
     3481 gaagacatca atcaacagca ggatggaaaa accgcggata aataatgccg atattgagnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnttgagtcg ctagcatgag tcagcaagac ctatcgtttt
     3781 ggccatctat ataatctaat aatgaatttc tcttaggatt ttccattttt ttaattttct
     3841 tggacaagct agaaaggtcc tcttccaata tgtgtcgtag atttttgttg taaattgcag
     3901 cagctggatg gattgttatg agatactctt gaccgtttct ttttatgatt tttcctctat
     3961 tctctttgat tgattttcca ccaagaatag atgaataggc ggtagctcct aaaagacata
     4021 tgattcttgg tgcaataaga gaaatctgcc tatctagata aggtctacat gctgatcgtt
     4081 cgtcttcttc aggtatcctg ttcccaggag gtcgacattt tactatattg gttatgaaaa
     4141 cttgcgacct ctctattcca gctttttgta gtgctccatc taaaatacgc cctgcgacgc
     4201 ctacaaacgg ccttcccttt tcatcttcgt tttttcctgg tgcttcccca ataaaaatta
     4261 cctttgctga aatctgtcct tctcctggta cagcgttctt tctgcttctt gaaagtttgc
     4321 aaagggggca ttcaatgact tcactagcaa ttttttctaa cgtgtcactc gaaatgttat
     4381 tagcagttgc caccatttat cctcacatgt tccaaagggt tcgatatcct aacgcgaata
     4441 tagataatat tgttgttgtt gtttgctttg ctttgcacac tagacttcga attaatttga
     4501 caaaacatcg cttgcgcagc tagtaataca agatgcagga aaagcttagg aacctattgc
     4561 ctaattaact gtgtaaatat gttatcaatt gaccattcgc gtcctcccgt aagtgaagaa
     4621 gaaggagtgt aaatcatgtt gtttcgttat tatcactcaa ttgattcgac aatcgagtct
     4681 gaacttttga cagacatgtt tatggcatac atacttgact gaaccggtaa agcgatcgtg
     4741 tgcgtacctg gccacaagaa ataagagaat tacagctaaa agagagggaa gttaatgacc
     4801 tcaaaggata gtagtgcatc tcgcaatact agaccatcaa tttgtatata tgccaaagac
     4861 ggaaatgatt atactattgg agttaatgca agatgagaat tttagatcaa tgccaaaaat
     4921 tgatgaatct cataccatgt tctttgataa atttaactag gtagtagatg atgagacgag
     4981 attgattgga aacaggaatt aaggaaagag atggattgct tctgcaaaga aaaatgagtc
     5041 aatatttgtt gaactggagc ttagtggtgg agtgaagtag gagaaagaat aaactaatcg
     5101 tgcatagatt attcgaccaa gccagttcta acaaagttaa agcctgaggt tagagggatt
     5161 ccgtcgagtg tatgatagac cgggaagaaa ttattcaggt gattattgca cataatttgt
     5221 tagtagtccg tatttatgca gcaattttct atgatgctga aattcaggat ctagtaactt
     5281 gacaatgttc caaaattctt tcgagtgatt gagctccatt agatgagcta gttcatgtat
     5341 aatcacgtaa tttattaact caacaggaag tgaagctaag agtaaattaa agttcagatt
     5401 tccgtttttg gaacaactgg cccatcttga atgttgagcc tttattgaaa ctctgttata
     5461 tgctggaaga tttgggtact tcttcctaat gagatcaata cgttccgata ctattttctt
     5521 ggtttctacg agataccatc gcttaatatc gtccttacat ctgcgcctat cagtgacatg
     5581 aaatgttatg gtttttaagt tatctgaaac tattacagag gctgcaaagt ctttagttat
     5641 tcgaagattg tatctcttgc ctagaaaaga aattgtgttt aattttagat tctcctcacc
     5701 acattcattt cggagtttcc catagtattc tgatgtttct agaatccact cttttttgat
     5761 gtctaagaat ctcattacct cgtcagcttc ataattaata attggaacta cgacataaag
     5821 tccaaagata tttgctttca agcataattg ctttgctctt ttacttttct taacttctac
     5881 gcttagtatt ctaccgtttg agagatgcac aggcaaagat gacaagtttt cgttatcttc
     5941 tttactcatt cattaacgtg tattttaatt ttttaaatgt agtcaaagag tggttttgtg
     6001 gtgacattct gcttccaagt gtgtgaggag ctatcgcaga ccctctccac gtcggtaacg
     6061 tattatagtc tttagtaact attttgcgaa accacggatg ctattgcagg gtatgcattg
     6121 ctatcttaca ttatataatc ccagttcgcc agagtagtgt tataaaagaa catcaatatt
     6181 tgtcttaaat gtaattattg ttactcatgt catcctaaga tcgagaaagt agcaagggca
     6241 aatgaagaaa ttaatcaaat attattatgg atataataat caagaaggag caagaaagca
     6301 atagtatcca gtaagatttg tgactttaat aatagcaaaa acaaaaacaa ctaagaagat
     6361 tttgctatat gggtagaacg attacttctc ttcgaatatc cagtgtatag gagtgaaaga
     6421 attgaaagca tttcgaaact caatagatat atcaggtaga aaactatttg atcaaatatt
     6481 ttctgatgta ggcaccagat attatataac tacaaagcta tcaaataaga gaaactacta
     6541 taatacttgc cctcttttta catgtatttg ctgacatgta tcacaggctc tggtctaggg
     6601 cagcgcgggc aaagtcagga ccaacagctt gatatgttga tatttttcgc tggtgccttt
     6661 ggctatgtag ctgctggatt ttgtggaaac agtcgtaaat ttgagtcaat taacatttat
     6721 gctgcatgca ttgtgaaaac actaataggg cgcgtcctct agctacctac taactatggg
     6781 caaattacct ttagctcata agaatgaaga aaaagaaaaa caaggagaac tagtggtgca
     6841 gaaatcaagc caagcccttc ttaccattga agatgtgtgt cctgtatggt ctaagaaaat
     6901 aaggcacaca tttgacgaaa cagacaagtc tataatgtca agcgattcta aatactgttt
     6961 agtaggtgaa gcttggggat ttagcggaag acatgcaggt tactatattg cccctctgat
     7021 tccttttcta gggtgctgga cttgcgtaat gtatggacat acgatgggaa aactttcccg
     7081 gaagcgggaa tcacaaataa gagatttcga acctataatt gcagattttc taagacactg
     7141 gaatgaaaag cataaaaata tcacagagaa gctgaagtat cgaaggaaaa agcatgtcat
     7201 tttctaatga tgatattatg acaagaatat ccagaactta gaaaaaatta gatgagtatg
     7261 agaagaacag tttacaaaga ttgtcttaaa taatgactag tgaagatcaa gaagaaagtc
     7321 aatctggtgc cgataccctt ctaacattgt caaacattca atatctcgta gaaggactgg
     7381 attcgttact acaaaccaat ctcgatggat ataaacgggc gaaagttatc aagttaagag
     7441 acgaactgtc ggcttatatt ggcaatatgt ttagtgacta gtagtagaac attacgttcg
     7501 atcctcaatt cgacaaatta tatacagcat taatgaggac ttcatttcgg tctgacgatc
     7561 ttgctgttgt cttaccaaaa gatggtttag catggtaaag agaaaaaaag gtttgtgcag
     7621 gactaatgcc tgaactaact ttacctctta caacagcccc taaaaaatat taatccaaag
     7681 agtttgcaaa gattacaagt ctccagctgt cacaatgaag cattaatgta ttcgttgcga
     7741 agcattagtt gttggtgaaa aatatttaga taataagatc tacatcatgg tttggattct
     7801 agagtgagta gcacttttgt tgactgaccc cctttcgcaa tatgttagtc ctctataggc
     7861 gaaaaatatg gagatataat atttgaaaca ttagcaactt tagatatcgc tccaacattt
     7921 gatagtgtag tcattgtctc tttcagaact gttttggata tttttccgtc tgtcgaatat
     7981 ttgactgact tcataaagct ctggcacgca tcttcagata atttataaat tctcatcatg
     8041 atgcccaaag aatagtctcc ttcagcgtga aaaatcttgg ctgctccttg tattgagtcg
     8101 agaacagatt ttatttgatt ggcaggcatt tctataaaat ctgttgtagc agctacgatg
     8161 aagcacggcc aaggtggatg aacttcacct atcgctctga ggataccgct atcaaggaga
     8221 aaacttatag atagcaattc ctaaatgaag caatctataa taccctttct aatagcgttg
     8281 actagtgagt ttaaatcacc cactggttta attcttatat tatttccctg ctcttcgttc
     8341 tgactaacta gccaaccttg gtcttttgcc aatagaacgg tgttaatatg agatcctcca
     8401 ccaaatctcg ttattccaaa agtctttcct tttagatcaa taacgctttg gaactgtgaa
     8461 tcatatttta ttacgacaac ccatctcatt tgactctcaa cgaatgttcc gacaattcta
     8521 aaatcggatc catttgagat agatgctacc aatccgtctg tcaatcctat tcagatctcg
     8581 gatttaccct ctgaaagtgc ctttgccagt tcaccagttc cacctgcaaa ttcctttatg
     8641 ttaacatgat ttagagcata ttttgtgaat aaacctgtat cgattatgat atgtaatggt
     8701 gcaaaatgaa tatcctggac tattcccaat gctagattct tttccatatc gtcttgcatt
     8761 gatgagtaat gctgataatc ttttcgaagt tgaagacaac ggtaccttca aagccgtcat
     8821 tattcgcatg aacactttac gtacaaagtc acctctaata cgatataatg gaatgaacag
     8881 aaatgatcac tgcttgttaa caatatgctc tcgtctgaga taaacttcta gaatttcaga
     8941 gtcaaagcat tttgagagac ttggaatgtt tcgggaagaa cgcctgtcgt ctctctcttt
     9001 tgattttata gagaccttct tagcctgcat atataaaatg tcatcctcat gcttgtaatc
     9061 agcatcgcct ttcatactga cgaattcatc taacattttg aagcttcccg aattcttaag
     9121 aatagttatg tagagcaagc cgtctgaaat gcatgcctac ggtgctgctt gaaatcctct
     9181 tcccagatat ctgccgttcg caactacacc cattgtcatc ttcgtaagta tattttgacg
     9241 tccgccatct atagaaacat tacataggtt actctcatat gtaggcactt acttgaaact
     9301 gtggaaccta tgcggctctt aatcttgtcc ctaatcttct ttgatctata gataatttct
     9361 gcgccaaccc ccatctctgc agcattaagg aaaattcgtg ctgtaacctc ctttgaagaa
     9421 tcatttgaat gcgttgatac agacgctgct gatgctgtta ctgctgcatt gacatcgatc
     9481 ttttgcggtt taccatttac gaatctttgg caacattcta caattctctt aggcaaatct
     9541 gatgacttgg ctagtacatt tcttgttcca catggaacta gagccatcac tgcctcgggg
     9601 tttatcggtt tcaagatagg cgatgcaggt gtagtttggt cattaccact actatttcct
     9661 aatgatattt cttcaaagaa gccgtttgca acctcattta cagttcatcg cctcctaatg
     9721 caacaatttt attaaatttc gttcttaaaa aattccttgt cagagtggtt ccatcaccag
     9781 atttttgact aaacgcaacc tcgggatttt caccaaaaat ttccttgatt tgattataga
     9841 gagtttccca gttctttcca gtcaatccac tacaggaatt tggattcact attaacactg
     9901 tttcagtact tttctgttct tgattcatgt ggatattata tcacatctta ttgtacccaa
     9961 cggtttattg gttgttcttc ttttttatgt cttattctgt acgttgcaga acactatgat
    10021 gggatcacag aaagaaaaag gtggcgcttt aagttaaagc gtctcttgct tttaccttat
    10081 ctttgttgag gttttcagag ctaggcttgc cttccgcatg atcttcttca gtcttcaatt
    10141 tcgcctcagc accctggctc gcattcttca gtttttttgc aatatcggtc atacaagcta
    10201 tatatagcat acatagttta atacactttc agaaaggtcg gcggtcgtgc tggaaatacc
    10261 aacgacccat tcgctaaata tctgctatct ttcataaacc acggacgctt gtctatatta
    10321 cggtgctgcg agtaacgatg aggaaaacaa acaatcttta tttttttagg ttaggacaag
    10381 gagatgtagt atcaatattc aaggagagag gcaattctag aagaaaaatc gcctatactt
    10441 gtgacgtcga ttcatacttc gagattagaa tctagtctaa tggaaagtcg tggcgatgat
    10501 cgccgggtca ccctgagatg gcccaaatgg gtaggtatga caaatatgac gtcaaacttg
    10561 acaacgacca acgcgacgta aaaaataacg ttgcgataga gattcatact agaagattta
    10621 atatgtaaag aatctagaag actgtaaaat agataatgag aatgaacgtc attctgatta
    10681 ccggtctggt cataagccag atagcatgaa tactaatagc ttactggaga atatttcgca
    10741 ctaaacatat tatagaaaaa gaatccgttt cgaacgacgc gccaggctta acgatgtagc
    10801 gtagactaat tgaagcacga aaacttcagc atataataca cgagaggttg taagaaaaat
    10861 taaagtatct ttagatgtgc gtgtgtgtac ctatgtacat cgagataatc gataggacaa
    10921 gttatgcctt cggtaaaaac atcgtagggg tgtactagca tgaaactcac agaattccgt
    10981 aaagcatgga tagacaagga aatacaatgt cgtatagaac aaataggaat gccaaagcag
    11041 gaaatacctc gcataattct gacaagaaaa gaatggcttg cgctgccaaa agagctcaca
    11101 catgggctta gaacaacgac gcataagaag ttaggaacca tcaagccccg atctagaatc
    11161 atgttcctca atgtcagatc acatagaagt ttacgacagc taagggatac gattattgta
    11221 gagctggtac actactggtt tcctgacctg aagcattata gccaattcca acaaatgaag
    11281 aaggcattac tgaaaggcaa aattccatac aaggacttca agattgaagc gacattaaag
    11341 ataccaattg agtaacaagg acgaactcct acatgcagag tctcgcaaat gaaatgcact
    11401 tttaatggtg gcagaggaag acgagtataa catacactgc tggccaccag gcttataggc
    11461 atacatccag gtttgtcatt tactaggaat gaaattaaaa ctcacggtga taatttctgg
    11521 tcttctggcc ttgataacaa cgatgacgct cacttcaact tttcctctag catttgcgca
    11581 ggagggtggt gggggcgccg gtggtggttg cggtaccagc ggaggtggaa gcagcaccgg
    11641 gaccggtggg ggtggtggca gtggtccagt cataagtcaa accacgtctc actttttcta
    11701 acgccgctga cacctactgt tatcctattc tgacaccttg taacgatgat atcgaaaact
    11761 gatatccgta ctgacagttt acgcaaaaaa acggaagaag tactggcgac ttgaggccgg
    11821 actgacatca agtacttaca aattaatccc agatcgcagt caatctcaat tttgtttctc
    11881 ccaacttcaa atgtaatttg gggcaacact gaatattttt attagatgat attcctttaa
    11941 ccatcctcgt ctcttctacg atctcattat tactaacaaa agacggtggc gttctgctag
    12001 caactggtcg ttgttctccg tagccaaaat atctctgtgt atatccaaat tcttgtctta
    12061 agatattact cttgttgtct ttctcgttct acttccctgg agtcataacc ggactcgatt
    12121 ccaaacaccg tcaggggtgc tgacagagct gctgacgaac acacacagat ttgtccatca
    12181 tttcagccta aaatgatatc tgatggtgtc gatcttatca tcccactact gatatgtctt
    12241 aacttgtcga cgaatttggt ccaaagcgac atcacgcttc aattcttgct tattaccggc
    12301 tttaagtgta gttggaatgc actaattttg ttaactgata ttccttcgaa ttcctcgggt
    12361 tcgtagttcc aaacacggta agtaaattat actgtttgtt gtcgtctata ttatattctg
    12421 tattttcctt acttgaattc accttcgttt ttcaccttgc taggtgtgat atttggttga
    12481 tttccagtca ataaggaatt tctagggtag ttttgcattg cagtcaacaa attgtcattg
    12541 tcttacttcg ccgttactgt tggacattct gccgagtggt gtaatggatt cacgactgaa
    12601 catcacagga tttcatgaga catttgtaag atcatcgtat ggataaagaa taaaagggaa
    12661 acaattggtc acaataatat atgataaact aaaatccaga ttatgttatg cattatgata
    12721 tatttctata aaagaactgg tcagcttgcg tcacgtgttg ctggtaccat cgtcgcagtt
    12781 gtccgcaaga tcatgctgga gaataggact ctgaacatga tcgtcgtgtt tttattagtt
    12841 gtcataatgg gttaactctt acattattct gttctacgta acatgcgacg cacttggtcg
    12901 tagatcgctc tgcccaatat agcacagtct gttcagtata tcaataataa caataataat
    12961 atatcaacaa ttatgtctag tatacattct tatacctcat tgttctatat aaccaatgag
    13021 agtgttatga gtatcaacat tcttagtaaa aaagaagata atttagaaaa aaaccaggca
    13081 acagatcaaa tatgcccatt agtggataaa gcatactacg tcgatacaat taattacaaa
    13141 atagacaaca ccggatcatc gttcgacgat gctgattaat aggaaatagc ctacctgtta
    13201 taacaaccac tccgctagcc tacttctttc aaaatcgatt ttcttttcat ataattcttg
    13261 cgatggtggt tcaagcttgg tgatgggacc tgcttcgttg taaaaatctg cagtaatata
    13321 tccagccttt ttacctgtct cttatacaca tctccgagcc cacgagac
//
LOCUS       DAOR01000126            5934 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_14402, whole genome shotgun sequence.
ACCESSION   DAOR01000126 DAOR01000000
VERSION     DAOR01000126.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_14402"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1936 a         1013 c         1179 g         1806 t
ORIGIN      
        1 actgaaatga attcgttctt cactatcttg ttatatgcct ctacttttcc ttgcagctgt
       61 ggataggaat agtgaagagg aaaagcaatt cacttgggat aggtcagtta actgcagcgt
      121 agctgatgaa gactttgatg tattaagtaa cacaacaaaa tatgaaaata aattaattca
      181 cgaacatgat tttgttcagc taacctccaa aggagtagac aactttctca tttgttgtat
      241 tacttgtggc tcatattact gtaacatgtg tggtaagaaa ctattttagt tggagataaa
      301 ggaacgaaag aaattacaga acaaaaaaag tggttcaaaa gataaagagt tgctaaaggc
      361 aagtcggaat tcacaacgtt ttattataca atgactttac atatatcaca cacattagat
      421 aatagttcta cgattcaaaa catactgtta gcaatctcgg atgataattc tttagcaatc
      481 cttagattga tagctgaacg agatactact ggagtagaga gctgcatcat ttgcagaaac
      541 ctacaactta ctcataaaaa gtattatcaa aggttagcaa ctttggtcag gaatggtttg
      601 ataataagaa aagacggtag taaaaaatat atacttactt cacttggcaa gatagtttat
      661 accgcactaa tatcaattca atatgcgctt aataatattt ggagattcag agcaatcgac
      721 gcaataaaag tatgtgataa cagtgtatat ggagacattg acaagattgc tagtggttta
      781 gtagacacat tagttcataa agaccaaatc aaggtaattc ttaagaaatg aaaccggagg
      841 gagaaagata caattagtgt tatgatcaaa aaaacgaacc cagcacccat tacagtgtga
      901 cttctgaccg atatacgtag ctcctgtaat cttggtaatt ctgaaatata gctttgtagt
      961 cttctgctcc tctgtcattt gaaatggaga aaccaactct ggttctcttt caagttttcg
     1021 cggtaatgga tcactcctta tccaaagaat taatcttcta taaccttttg gtattaagac
     1081 actttgataa agctggcagt aattgcggcg aattctggaa tagtctcatt aatggcttgg
     1141 ttagtagcag cagttgcgtt tattgtgttt gtcgcattga ctaaattttt aggtgttact
     1201 ataaatgttg ttgtcgcaga tcctttatcg tatccagttg ctgtagcatc caccgttacc
     1261 ttaaattgtt ctggatctgc atttccgctt attctccaac tttgaacctt gcactgtcgt
     1321 caggcctttc aggaacaggt gctcatgggg ttgctaaagt atttgcacca gaacaagaag
     1381 cacaagcaag tggtggaagt gcctccagct tcgcaccagg aatagtgaag caaacaccta
     1441 caggttagcc aactaatctt ctcttttttt actttcgttc gttctttgta tctccaatac
     1501 ttttattata gcagctccta gcctaggctt cagtcgtaat ctattcctac aacacggaca
     1561 gcgtaggcct tcgacgtaga tgacttctgt ttactgctta gttagctaca cttacagtta
     1621 tatcccttca gcgttcgagt aagtaataat tatgattcta aacgatgatg aacttcggat
     1681 gtatcgtgac aggtttctat gtcggttgta tcgcacagcg caaggtattg tagatagacg
     1741 ttttaataga tatgatatct tcatggcgac tggtattggt gccatcacag atgtccacca
     1801 agctacagat gagattgtgc aatctctgca gaattatggt ttcattcata caattgataa
     1861 tgaacaaata acactgagta tggctggttt agctgaagcc gaaagggtat gcgaatgaag
     1921 aataattacc aaaaatacga gtatgagtcg tttttactta tccgacatga tatgtaagaa
     1981 ttgaggtcgt cctgacaatg ataagtatca aactaaagag ctaagaagtc ctaggatcgt
     2041 ctgtatggaa tgcgtgaagt ccagccagaa gactagatac atatcataat atttattgca
     2101 aagaagagcg ttatatttta taatccaccc gattcgcaaa ttcaatccat catcagcggt
     2161 cggtgccacg ccaaagcaaa ataggctggt ttatgctagc tagttctaag gttattagca
     2221 gcactacaac gataggcaat tatacaaaac attcactaga tgcagatcga ctcgatggct
     2281 ctatagatca tagacgctgg agcagcatgt gctaatttga atgggattcg tctttatgta
     2341 gagagagggg agagagcatt cgcggaattg gtcaaagaca atagttggag atatattaga
     2401 tcggaagaga gagtggtaac atgatatcag acacagatca ttttgctttg cttaaaacaa
     2461 tcaagggaat aaaggataat gtagacaaga tgtatacact aggaaacgat gaattgcagt
     2521 acatgagaga tgaattttta ggatatctta aacaacttgt tgatgtagtg aacggtcttc
     2581 attcagaaaa ggaggaacta acagatgcaa cctcttgatt tagatttggt agatttgatg
     2641 ataagatgac aaacttacct tcaaacaatc ctaatgatat ccaggctggt tacaatgcta
     2701 ataggaaaac tgtaaagact aaggctaagg ctaaaggaat gttgcaagca ggggaacaag
     2761 ttatatttca caaattatac acgaaaggca tatttcatcg tcatacgatc agtgagattc
     2821 catacttctg tatcatacat cccactatga taagaaaggt tattgttaag taaacaccct
     2881 ccaattttgt tactaacgga aatatggcct aatgagcagg aattcttgac cttaagttat
     2941 gcctcagtaa ttccaatgat agttagtcta cttacttcta tgtgccaaca gtaaccgtaa
     3001 caatgtagtg gcagaacaca aacgagagac aaaccattag ataatgaagt gatacctgcg
     3061 gctaaggatc gaatttgcta ccacaaataa tgaaaaagaa ttattacttc attgtattat
     3121 ggtagtacta ttaattctcc tctcattgtt ggcagatgat acttgtttaa tcggtataag
     3181 ataggctgac atatgttaaa ataattcagg cgcttattat aaagaggttg aattttatac
     3241 tacaacgata tgtcacgata aaatacctcc agattaattt cggcggcttt tcctacgcta
     3301 tctggcttcg atttatttgg atcgaggtca ttgaaatagt gtgtaagctt ggatagagat
     3361 atcctctcca tgattaaaga cataagattt tacaatataa ctctaattgg caagtgcgaa
     3421 aactattatc cctactgacc cttgaggaat atggagtcac atatcaacaa taataatcca
     3481 aaaaaagatg ctgtgcatta cattgtactc ctcacgttct tatactagac tctaatcctg
     3541 ttatacagga agcgcgctta ttacgatgag gtcaagctaa actcatgtta agattggata
     3601 taaacaatat ccaggaatgt tgccatcaat gtactagatg cgggattttg tataactgta
     3661 aggatattga gagatgtgca ttctcctttt tctatggtaa atgctctatt tgtgatgcct
     3721 taatttctgc cttgaagatt ggccggaaat tcgagataat gttctaatct tgctctacga
     3781 tacttctcga attgtgacag atacacttag tatgggttgt cgtacaaggt aactcttggc
     3841 taacagtata tatatcaatt cagaactata gattagttat catgatatcc gaattacaag
     3901 gacaaaatta cattaatctg gaaacttacg aaaaaaatgg acaaccgatt cggacgccag
     3961 ttgcatttgt tcaagacaat ggggtgattt atgtcaggac ggataagaat tcaggcaaag
     4021 taaaacgttt acgaaacaat ccgcatgtgc gcatagtgcc atgtgatatt agagcacggc
     4081 cgaaaggcta atgggttgaa ggtacgatcc agattgctag tgaacatgaa tcggaacgtg
     4141 ctaaaaaact tctaaacgaa aaatacggca gcggcttccg tggaatgctg cttaatataa
     4201 tgtacaagtt tagaaagatt gatttcgttt ttctttcaat tcgctagcag gtctgttctg
     4261 actgatgaat tgggattcat ccccgtgcat cgagatgcga gccgcttccg ttatgagatt
     4321 ctaactgtgt atgcaatgta ttgcaatatc atgcacatac ggaaaaccta taacgccgag
     4381 ttatattaat tcacttacag ggagattatg ggtggcagtc tctatctcct tttatccaga
     4441 gcgtctttaa tcaaaaatgc cacatccgga tatgcagtag tagatactag atactagact
     4501 atctcgcagc cctgaaaata acattatccc tgtgtgccaa gtcagtaagt cgtgaccaaa
     4561 agaccatgtt cagtgaaggc tgtgaaggct atataggaga gccccccttg tttttcagca
     4621 ctttgaagta ctttatagtt atttatgagg catacaacat cctcctttaa tgtaatttct
     4681 gggggatgct cctctggagc tctgtttcac ataaacataa tcatgggcta gttgtgacat
     4741 gcaaatcaac ccacgttaac cgactgacct tgctctagat ggagagcaga tttattgcaa
     4801 tagcaaaaat atattgtatc atataaaata ttgatagttt gaagatattc tctactaaat
     4861 acaattaaat agatttttta gctacaaaat acatatgaac ttatgtcata tactaaaaat
     4921 attaaataca ttcttatgga atgaggttta tgattaatta gtgttatttg agagggtcta
     4981 ttattgcact ataagagaag gagttggtct gaaagcgtaa acgtcttaaa aaaagagaga
     5041 attaagatga gacggaattt aaaacgaata cgtttcattt tatgcgagtc atgtttttgg
     5101 tgcgcatcgt gccttaacct aggaattaca gttgatgaat gtcgatggtg tagtttcttt
     5161 ataaatgccc cctagtttgg ttcaaaacaa gcttcgactg ttaggcctta gagtagattc
     5221 ataaaaccaa aacctaatga attgcgctct ccaagtccag aacctaatgc aaactgaatt
     5281 atatctttgt cgactgcggc attaaaacca aattcccaga ctgttccaat tacgacctgg
     5341 tcaaatcctt tcataaacac tcttgtagaa atttgttttt tgaatttaaa actatggaat
     5401 aagtgcgata tttctgacag gtctttgccc ctaaaattat ctgtaaaatt ggttcaattg
     5461 atattgggca tatttttttg ataagttatt ttctagttgc cagatgaata gatcaattgg
     5521 atgatcactc ctccagtaaa tataatcata cttcgtttgt tcaaagtcat actctttgta
     5581 tgtttcttat ggtcggttcc tgaaggatat ggcgggccct acggtccagg atcatcagac
     5641 ataggaactg gcatactata tgctattgtc gcctttatgt tactaatcat caatgctacc
     5701 ttcaggccct caagatatag tttagatttt attattgaga gaaaatggcg ttggtggaag
     5761 aagatagctg aaataaaggg gtagatggta atggtgaaag gcgataatgg attggaatat
     5821 ctgaaataga tgttggatag agagaataca tggatagacg aacatataaa actgcgagac
     5881 ctcacacaga agaaaataga gaagttggag aaaaaaatga aaatacaaga agac
//
LOCUS       DAOR01000127            6205 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_84428, whole genome shotgun sequence.
ACCESSION   DAOR01000127 DAOR01000000
VERSION     DAOR01000127.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_84428"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2958..3150
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2025 a         1101 c         1087 g         1797 t
ORIGIN      
        1 tttagttgtt gatcgaagac aatccctaat tatagaaaca aaggatgata caaaagataa
       61 ttcttacgac gcagcgggat tgggggcata ctctgatagc aagccgattg cgttatcata
      121 tgcttcaatc tttgatagtc tttggaaaca aagagaatta tatgaaaaat taaatgaaac
      181 gtatgagcag ctaaaaatca ataataaaat gcaaaaagaa tttctcaata tagcggctca
      241 tgaattacga actcctatcg agccgatact tggattaact ggagttctac gttctgataa
      301 aacaatggat agagcagaag aagaagcatt actggatgtt atagctagga atgcaaagag
      361 gttgcagcga cttgctgatg acattttaga tgttacaaaa attgaaagcc aatcattgga
      421 gctaaaaaaa gaactaatca atgcaaatga tgtgatatca aataccgtac aggaaattaa
      481 caatcaaata gatcatgatg tgaaagtaga agtggtatgc attcctagag accacgatat
      541 tatttttgta gaagcagata aagcaagatt aactcaagtt attttcaatc tcttaactaa
      601 cgctgttaaa tttaccaaga aaggcggcgg aactgtatcc ataactatag aagaagagaa
      661 acaagacaac ggtccccaaa agcttgccgt tgttaccgtc aaagattctg gtacaggaat
      721 aaatcaggag atattgccca gattatttga gaaattcgct tcaaaatcgt ctcaaggaac
      781 aggtttagga ttgtttattt ctaaaagtat cattgaggct catggtggaa agatatgggc
      841 taagaataat actgaagata aaggagcaac atttgcattc agtctaccat cagcaaaaac
      901 aagagttaag atataatgtg ttaagatata atgtggtcga ccatcgataa aaataagaaa
      961 agaaggatat cgttagtagg cgatgattct gacgttattt tacgttaaaa acggtcttag
     1021 agtagattca ttcattgatc ctacttgagc attgtagaat ttcaaacccg ggaataatgc
     1081 tcaataattg accctgaact cttcgaattc accagtatac ttttcatact ttatatcaac
     1141 atcgttaaca gtagcctttg gtgggcctgc atgacaccat tcaatcacct gtccaacatc
     1201 tgcctcatat ccttctaaaa gcacctccac tcgtccatct tttagattac gtacccaacc
     1261 cgttacacca tgcccagtag ctactattcg agtgttttgt cgaaaataca caccctgcac
     1321 tttgccgagt acgagaatat gtgctctgat tttcttgtta ctattgtcgt agtcctttct
     1381 ggtaaatgag gatggagagg atgacaatat aaccaaccaa tctctgtaag taaaatatat
     1441 ataaactcta ttcggggata aaaaaagcgt ggttagaata tatctcctcc ttcttctata
     1501 aagagtcgtt catatgacga tccttttcta actagtttaa attctccctt ggcagtcttc
     1561 ataatgacag gtggttttat ctgacagtga aagggcatat tgaatacgag ggagtacgct
     1621 cctacattat agaatgacat atatcttcca acatctgctt cattcaggct cgatactttc
     1681 gataagggaa aaatgtcata ggtatcacat agcctacctg ccaaatggat gttctttgtg
     1741 ggtagctgac ttgttgtctt gactttgttt ttagatattg aaactatttc ttgtggatat
     1801 tcttgtttta aaagagctgc atctaacaat aaatgataac ctgcatctaa tatgacaatt
     1861 ttgtgttcac tatattcctt agtatttact atcttagaaa ccaagatcga tgattcagct
     1921 acaagaaacc tgccgctttc gatcatcaat tcgaatattc cgtgagtgtc tactagttgg
     1981 ttgagtcgag agacaaggtt ttcagccatc tgcgtgggac ttggtactgg ttcgtcatag
     2041 gacactggag tccctccccc aatatcaaat gtattaacct ggaagtttgg atactccttc
     2101 ttcatttttg tcataaatgc atccatcttg tcaagcgcat gtataaaaca tgagaagtct
     2161 gtaatttgcg accccagatg aaagtgaaag ccctcaaatc taagtaaatc attgtccatt
     2221 aggtgtttga tcattttggt tgcgctatcg atggttccac cattgaacgg aactccaaat
     2281 ttgccgtttc tgtatgatgc acgaactcca gctttaactc ctacctctgg attcactcgg
     2341 atcatagttg gtacctttac acctattttg ctagcggctt ttgccaaatt cgtcatccca
     2401 ttaaaggaac tcacgacaac tttccgtaca ccactttgta atacctcaag gtattcttcg
     2461 aattcctctg taacgcttgt ataaatgatg ttttcgggag ctgctttgca ttgttttata
     2521 aaatgaagtt cgccaatcga tgtcaaatcg aatgttatac catcagttat gaatgtacgt
     2581 aagatcgctg ggttaaagtt agctttaatt gagtaggcga tcttcagagg tccaacgaac
     2641 tttgtatatg ctgcatgcaa ttccttgact tttgcgtgca gcgtatcttc gtcaatcaaa
     2701 taaagcgggg tgccgtactg tgcaactaat ttgctgattt catttggttt tacaaagcgg
     2761 tttaacgctg gtgccgttag atctagattt tccaccagtt gaatctgaga attctccacc
     2821 aatttctttt tgttatttta ttgctaaaac atataactta aaaacattgc ccccccagta
     2881 aatttattac ttttggaata acaaaaaata aggtcgtggt ccataatcga ggacatattt
     2941 ttcctatcca gccccctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcctatccag ccccctaaaa tgaatagaag
     3181 tattattgtt acgatccaaa acacttctct accaaattcg ttcattgttt tgtttactgc
     3241 aggatcatac gaattgttaa tgtccaaaaa tgctgcataa tatgatttag cgatgggaga
     3301 tatagaacta gcattgtcat aaatgattct tggagaaacg attgcagcca gtacaataaa
     3361 tgtgatacga ataacaaaag aacctgcaaa gtacctatct tataattaat aagcaaatca
     3421 gtgcctgtcc aagccatcac aattagccaa atggaaaagt ggtacaaagg acccagtatc
     3481 aatgtagtcc atgagacaga ttattttata tggccttctt tacgcaattc tgcaagtatt
     3541 atcattaagg tgtcataatc tatgtccact tcattcagaa taacagaatg atctactttt
     3601 ccagatcttc tgataaattt ttcgaccatc atctttttct tttgatactc cctacctctc
     3661 ataatcttca tactctaata ttaaaaatga ctagattatt gtctttatct atgctctgga
     3721 aagaaagaaa taaacttgca tgatttctat gatcagattt ataactggca cataagcaga
     3781 aatctaaaga tactgacctg caattaaaga gttctaaacc caacataata attaaaagag
     3841 agggtttgaa gcatgcgata acgaaatgat gaaaatcttg gattcgaaaa tgtatcagtc
     3901 aaagcagcaa catttgatga gtgagaagaa atagtataat aatatgatgg gaactaaaaa
     3961 ttagagttcg tttttttctt acactaacta ccagcgtccg caacatcttc aatcatcttt
     4021 tttccacttc tttttgaata atagaatcta aaccacaaca tcatgctaaa aaagaccatc
     4081 gaaatagcat gtatttctaa tccaacatca ccttgtagag cggaaagtac agggattcca
     4141 ttaacctgaa gaataccagt tatctctcct aaaatccaaa ttattataaa aatcgacatt
     4201 tggaattgaa aacttctaat atttctagat cgcactgcca gccatacaaa tatacccaat
     4261 gctaccaaaa gcagcaatgt tgtaatgctg tgcataagaa gaaataaggt acccggattc
     4321 catactacaa cagtcgtgtt ttttactccc atattttttg gttttgcgaa accagaataa
     4381 acaattccca aagaatatca atactgcaac agaaagtggt tcaaaaactc cttctcctaa
     4441 taaagttaag ccataaaatc caacatatgg taaacggcat gtgtcagtat aaaaatcgta
     4501 agtatcagtg ttaatttcct tatactgttt tttaatttta gagtaagata aattggaaaa
     4561 ataacaatgg cactgatgaa atgaagatta gtttctaaca tttcggtgga ttacgcagcc
     4621 taaagctggc cgatagacat tgcatttctt cgcatagtat gtctacgtat taatgcctta
     4681 ataaagtggg acataacttc cagatatgta agctactcat cgaactcact ggtagtattc
     4741 gcctctggtt ttgtaatcgt aacaaaggcc aaccttattt agctttatca aattaaggct
     4801 ttagcttgca atatggttct tcttgatctc gatataccaa atttaaacag acttaaaaaa
     4861 gattgattac agcaatcaag gtttgtttta tggcggcatc tattgatgtc cagttgcacc
     4921 cttagggatg gtgttggtac tggtgatgtc atattactgc ctgtcatatt actacctgcc
     4981 attgtcttag taaaactagt ttggttaccg ggcatcattg tctgagccga cggtgacata
     5041 ctattagagt tcattcctgc tagtacgctt ctaccaatac aactgcaaaa atcgtgacct
     5101 ctaggttgaa aatttgtttc atcgcgtaca taatacgaag tatcaattca aagattgaga
     5161 gcgcattaag cactaacgat attaagtagc cgccgtcgtc tccgactatt gggtgtagag
     5221 agcccaattc gagatacata aagcttagtt ccaccaatag aataatacat aaaacgagct
     5281 cgactatgcg taagaatgta gctgtgaaaa tcataggaga tacaaaagat gatagcagcg
     5341 ttgggaaagg aaataatgaa gcaaagaaaa actacaagtt ttaacacatc tattttgtct
     5401 gggtaatcaa gttcagcatt agaagaaccg tagattacat tagtagaagg atatgacgga
     5461 atttcaagct aatttaggga tttgaaccga gagttcaatg gggccagtgg tccccgcaac
     5521 gatatcgctc ctaaaaaagg nntacgaagt atcaattcaa agattgttag aaggttattt
     5581 gtataagaaa tgtatacttt cttctgaaaa tcagaaaatc gaccagggcg gggagccaaa
     5641 aattatgaag gaaattcaca acaaagcagg tgttagatac cacgaggttc ctatctacag
     5701 attgctacac aagtggtttc aagccaatag taccaaaaag ggatttgtca atattggatc
     5761 aaaagaagag aaatgaccgt tcaaaaaaat agaaaaaaat cttaaacatg cataagccga
     5821 acgaatctat attcctacat gacgcatcgg ttagaagaat gatgtgggca cataagaaat
     5881 acatctaata gtagtaccta cgggatcctg aagaaaacct gtgtatcatg ggagaattag
     5941 gtatagatag gagacagtta ttcgaacaat ataacgcctt tgaccgtact tagactattt
     6001 gaagaaactg caaaataagt ttcataagtc agaataacaa caacttagaa tatcaaagaa
     6061 tgagttttga acaaccaacg aaatttgaga gttaaaactt gttgcacgtt aaaaggaacc
     6121 aacgttaaat ataaaaaaat gctacggtcg aggcagagca aaattttgac aaagcaaaaa
     6181 agcagataga ctgattgttc tcatc
//
LOCUS       DAOR01000128           39818 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_30556, whole genome shotgun sequence.
ACCESSION   DAOR01000128 DAOR01000000
VERSION     DAOR01000128.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 39818)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 39818)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39818
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_30556"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    465..657
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12290 a         7573 c         8027 g        11735 t
ORIGIN      
        1 tactatgacc ctttactatg accaactata ttcactcggt tctgtcctgt ttcgctctta
       61 atctctccaa ttttctttga taattcgttt gcatggtctg cagcggatcc acatttgtca
      121 tctgattgtt gaaaagttat aggaaatgca ggaataccat cttttttaag caaatcctgc
      181 catttattcc ataccgctgc gtctgataac catgtatcaa gagtacagaa agtagttttt
      241 ttggtgcagt ataacctaat gcagcactac tattggtatt agatcgttgg tagtaggcta
      301 tcaccacgat agaaaggaat ggtcctacca atactggtta ttatcatcac tattatcact
      361 gaagcaatta cggatacaat cccaatagtt ttcatcaaaa gattctgcgt cagaaagagc
      421 tattagcatt tcaatttcac agtctagagg gatagatgca gcgannnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc
      661 tagagggata gatgcagcga aggcccaatc cttcttacac acaaactcta ctttaagtaa
      721 ctgtgaacgc gtgatacttg cgtctagcca acattgttta accaatattc tattgtcggt
      781 agttcaacgt aacggagcgt atgtcataga aggttttttt gataaagcaa tatacggatg
      841 atgcaatgat tcattttttc tttccgagct ctgtgtaaac ctgtaggcac accactcctt
      901 tagttaaacc tgcgtatgga aatactaatt gactcctcct atttttgcta gatctttgaa
      961 cgtccaagtc ctgtacctgt tcattactcc gactgatagc ataaaggtat aataaacaat
     1021 aaaaaacgtt tcaacttatg tcgcaaagtg aatggtttac tacctattta gaatacgaga
     1081 atttgaagga tatgctaaat gttatgcttt atacatcaca atcctttatg actattacac
     1141 cactacttta ttacataact tatcgtgata aagaaatact tttcattcat tctggtatag
     1201 tcggtggtat tgttgcccat tatcatatcc ttaacgaaaa accaaacaag aaatttgtgg
     1261 aactggataa gatgaccggt gagtttaact tcctaaatgg tgttggtacc aatcctcatt
     1321 ccatttacat tcctatcgtt agattaatca agtctactct tagatttcca atagatgctt
     1381 tgggcacagt ttagaacttc atcatgatag tgtcataata gaattaaggt attgacttgc
     1441 tttgatccaa ggacaccagg agtagaagag ccaacaggtg tattacttcc atattcgtgc
     1501 caaatgatcc ttgaatgtct ggattctttc aatctaatct tggaagcacg agtgtaaggc
     1561 gtcgcgtcga tcccaactgg aagatctaag acgatcacaa ttaaaattat ttggaggtgc
     1621 agcttttcaa cattcctttg ttaaactttg tactagcagc tttaattgtt gattttttct
     1681 cagttttcct actggtaggg ttgaacctct tagttgcatc ccacagcagg gacaaaaaaa
     1741 tgctcttgag agatctaacg ataactccgg aacatcgttc tagacgaaag cctgggcgga
     1801 attgcccaac ccgatagaca tggggtttat gtctaatttc tcaccgcggt atagtaatat
     1861 ctcggtgaga caaacggaca tatgaccgaa ctgaaattaa tagagatatg tcgaataatg
     1921 aaagcccggt cagttggcag caagatctaa tagatggcat aaacaaactt gaaagaaaat
     1981 tagacgacta caaaggacat atcggcgcta accaaaacgt gaaagatgaa tactttgtcg
     2041 cgttgaagaa ggttgctgaa gcaaaagagg tcattagtag gcttgcagag acgttcaata
     2101 aggaatatgc atcgggatag ttctccgtag attgtagctc caccatgtct cgatggtctg
     2161 cacgaagtgg ttgttgccga tccactttca actttggagg tgataatctt gtcgcagtat
     2221 attctgaacg tagcatatca ggtttctgga tctgggttcc tgcaattcaa ggaagagatc
     2281 actgcgaaac atatcgcgcg cattgagcct cttcatttat tctgatttca tacagatttt
     2341 ctagcttgcc tcttttatta catccattat tagaatagca gcctgccttt caatacaggg
     2401 ctgctaaatt agagatttca ggtagtagta atcggagcta aagtgtagcg tatatccgtt
     2461 gtatgtgcca attatttcca gtataatagt gtcgaaggtt tggttcagtt gccaaaattt
     2521 tgggcctacc ctgccgattt tgggtaatta tacagacatt catgatgaat tttaaaaagg
     2581 cgcaattcgc acgctcattc gtaacctttg tttttaagat tttgtgtaaa aataatgaaa
     2641 aaataatccc ctataatatt gtcaccttcg cctagagact ttaagtcagt gtgtcggact
     2701 cgatagtatt tctaaccaag gaatttgaga gggatgatgt ggtttcatgg tcttcctccc
     2761 ggaggaagac catgatattg tctgtatagg catacagatt gacaaagatt gttggtttct
     2821 cccctgacga gtgggcattt ataaaaatgc tgccaaatct aacagtcgat tgagcggaaa
     2881 ttgagctatt gaaagaatat gggggaattt tcacttggtt tttgatgcta cctgacaatt
     2941 tcggatatct atacttacat taaatgatga tttctgaaaa gaaaagtacc tgcgagcagg
     3001 aaccaattaa attgaaaaag ttacaagttt ggtcacaagt tcgctagaag gataatccaa
     3061 cattttcttc tgttcttctg tattgggatc tactacgaat tggttgtaca tcctattaac
     3121 atagacttga atgaattcct tttccttcag tttgacccac aaatcctacc gttatcagga
     3181 tttcttgttt ttcacctaag acaatcctac ccctttgagg agaggtgtgt agtacccacc
     3241 tgggttgctg ccctgggggc ggccctaatt ccgtaaaatc tatgccaacg ttactactca
     3301 ctaaaggata gttctgataa ttccagaata catatcaggt ttaatatcgt cttcttttat
     3361 tcctacccat gtcatccggg gaggttaaac cgtaattttt gataaaatta aattaaacct
     3421 gaataggcct tatttgcacg ctcatatgca gcattaattt ttaaaacttt tcataaaaaa
     3481 atacaaaatt aacgctgccg ccctcccggg agggtacgcc gtaccttgca tgcaaataac
     3541 aactatggaa ggcattttgc atatagaaat atcaactcta ggacaataag aacatcacca
     3601 cctgaggact ttcagaaacc aagtctttgc acgatggaca ccgggaatag tgaattccct
     3661 tgcaattacg acaggacagt ttaactgatc tagtagagac aacaagtggc tgttattgaa
     3721 gaagctaaat tttccaatag ttgcgcctgt cactactaaa tcaggttgag atgcgttaac
     3781 agcagataga atcaaatcgg caaatttttc actattcttt cctgtttcct ctgacaaata
     3841 aatttcatta aattcaactc cgacttgttc gaggtactct ttacgcttgt tatcttgagc
     3901 aacatgatcc tcgaattcct tagcgtcctc tacagattgg tcttcacgtc ttttatttac
     3961 cgctaataca ctcacattga actttccgga gtgttctagc cagctcgttg cttttagtac
     4021 gaggtctgaa tgatatccgc catcatataa aacaaccata ttctttttca gacgccgttc
     4081 agccgtttgt gcaacaggtg tcaaatcctc actaccttca actgaggatt caaataaagt
     4141 aaggtcatct ccggttgttc taatagcaag cacgttacag gtgcttagcg tttgaagttt
     4201 cttattattt ctgtatgttt caaagtcagt aattagcaaa tcaatttttt gttcttcaat
     4261 cgttgccaat actgcctcgc ttgtatcatg agaaatccta accagatagt gattcaaaat
     4321 cttctcttgt tctagcattt tttccaacgg ttcaaatgat tttctggcag catctgcgaa
     4381 cgcagttcct gcagatagtg gtgtttgatt tggcactgta attactctta atacattgac
     4441 ttcaccgtca ttttctttgg caacccttat tgcatacttt atcaacctgt caggattttc
     4501 tggagtatat ggaatcaaga ttctgtattc atttctggtt aaatgccctt cgcttgttac
     4561 aataggagca taatgctcaa tttctttttt gaaagtgtac atacgataaa gtgcaaatcc
     4621 gactaatatc cagagagctg agataaccca acttaaaggc tgtgtgacta gcaaatataa
     4681 cgcaagaccg agtttaagga agacacctat aattggaatt attggaaaaa aaggaatctt
     4741 aaatccatag tttagtttat cgccatacat tcttcttata ttgataacgg cgatatttac
     4801 ctgagtaaac agcagcaaga aaatgactcc agcggcaact gctatctgat caagaggaag
     4861 tgcataagcc ataactgcca ttattacacc cgatattccg atggcaacat ggggtgtttt
     4921 atgtttggga tgtatagtgc ttagccggtg tggcagattg tagtgtctgc ccatagcaaa
     4981 cgaaactcta gaagaagaaa atgtagtggc atttaatgct gctaagctgg agacaatacc
     5041 tccccccagc actataaacg ccccatatgg aagaaagagt gcagcagctt tcgtgatacc
     5101 cagatcaccg taatgaccta tgtatttcca tgctggaaca cctggaggaa atatcgcgcc
     5161 tatggaaaca aaggcaacaa gacagtacag aacaactacg attgctagag aggtaaaaat
     5221 ggcccttgga atgttttttt ttggattctt tacctcttct cctgtttgta ctattatctc
     5281 gtagccctca aacgcgatga atgtcaaacc catagcagca acaatcccgc ctatgccatt
     5341 tggcagaaaa tcagcaaaat ttgctttcca gtttggatga gtatgaatcg tccaaaaacc
     5401 tgcacctatc aaagcggcga tggctccgag ctgaactatt gtgactattg ttccagcttt
     5461 gcctgtctct gaaactcctt taacattgat atatgtaaaa gcagcaatcg aggctactgc
     5521 tattaactta tcaaaaggaa taattcctaa caggggatgg tctccaagaa tatttaccat
     5581 tttcaataga ctaaaaagaa atgacccaaa tcctacagcg tacaagcttc cagcaacgat
     5641 atgtgcaaac cacgccatcc agccacttat gaaagcattt ggccttggta atccttccct
     5701 aacccacagg taaccgccgc cagcttctgg tatagcggag ccgagctctg catatcccat
     5761 ggccgtaaaa agagtgatta ttgcattaat tataaaggca acaataacag aaccccctgc
     5821 gagaccaatt gctggaccag tgaggacaaa tatagctcct ccaactagcc ccgctatacc
     5881 gatcatcatg acgtccacaa aacccaatga acggactaag tgttcttgtg acggcgtgtc
     5941 atcgttgtag cgggtagaca aatattctat ctactctcgc cctgatatgt ccaatagtaa
     6001 ttaaaggttt agaaatttgt aaagtcggat ttcacagtgt tttatgaaaa agagtctctc
     6061 acgtttgttc atatgaatat tggaggatat tatccaacag aatagattcg ttatctccaa
     6121 taccttaatc tttctaacac tcctatccac ttacttcttt gtactattcc taaataaagt
     6181 aaagaagatc caataaagct tggaattgtt gcaccaatct gagaccattg aagaatgtaa
     6241 attggagaca gcgaggataa aagataatac agcaatacac caaacgacca tgcaacaatt
     6301 gccggaaatc caatttttag gacatttttt ccgtacatca tatcctgtgt atattgccgc
     6361 cgcttgacaa tatagtagtc tgacagaaca accccaaata atggtacgaa aacagatcct
     6421 atcaaaagaa taaaggtctc atactgggag ataggaatca tcatggcaag aacagtacta
     6481 agggctgtaa aaccagttat caaatgccgc tgtttaattt tgtgaaatat actttgagta
     6541 gaaactgatg aagaataaac atctgcaaat gcattagtag tctcgtgtaa aataaacata
     6601 aacaaaagaa acccgaaaaa tattgattgt attgcaagaa ttatcgcaac aacatcagaa
     6661 ttgccaacaa gtacgcctcc aaaataaaac aagatgttag taattgcaaa acccataaat
     6721 gtgcccctaa aggcgccctt gctatctttt gcaaacctgt tataatcagc tacaagcggc
     6781 atccatgata cgggtaaagc tattacaata tcgagggcag agaagaagga cattccagct
     6841 cctccagatg ctgatagcag tttcgaaaga tgtccagaca tcaaaagatt gataattatt
     6901 agaaacgatg cggcatatac cacccagatc gcaaattttt ccaaccactg tcttacgacc
     6961 gtaagtgggc ccacaatacc aagcagagta acgaaagctc cgaatagaat agtccaaaga
     7021 taatatggga ttcgatcccc tgttaggatt tccgtagctt ttgacattat cataatttca
     7081 aatacagtcc atccgatcag ctgcattaca tttaatatgg caggaatgga agcaccatga
     7141 ataccaaatg acggtcgggt gcttatgaga gatggaactg catgatcgct cccaatcttt
     7201 cctgcaagag cgagaagcaa agagcctgca atagaaccga ctataattgc aggtatcgca
     7261 tcagtaaacc gagcggctgt tagaagagat cccgcagaga aaactagcag tcccacgcct
     7321 aggcttgacc agagcacaaa gtactcgatt cctctgagcg atctatcttt tggcggaata
     7381 ggttctatac cccactccgg tagcctcctt attggaaaca tttgtctatt atctgtacca
     7441 gtaggtcata tgaatatgaa catgtcgaga cgttgctgat taatgaatac acatattagc
     7501 taacataatt atcaattaaa gttattgcat taatcgatta gagggatctc tactctgcaa
     7561 tatttgacta aacccttaga tgcatctatg gtggttaatc gcaacttcca aggaagtacc
     7621 tatcagagaa tgcaaaagga aacagagata tattatacct ttgcagagga tgtttgtcag
     7681 cggggtgggg aagccagatc cttataaaag tgaggctagg tgatcccggc gggctcataa
     7741 cccgcagagc ggtttccact tttgcatttc gcaagtggcc gcaggttcaa atcaaccccc
     7801 cgctactaat ttttcaagac gaggtaacaa accatcttgc tattaatttc ggtcttggat
     7861 taaagcctaa tgacttcttt tccataccat atctgtggag gggcttggtt aaatgcctat
     7921 gagatttcgg cgtcggaaca tagagcccgg tcaggacatc tctatcccga agcactggga
     7981 ttttcttggc attgccgtcc ttgtagcgac acctgtcgca ttgaaatccc ttgttctttc
     8041 cttccgactt cattttcttc gtacaatatt tgcagggcgg attagtcaga tcgcgtatct
     8101 gtacaatttt caagatagaa atgtattcca catttaatat tggaggacga ttacatgttg
     8161 cttttcggac accacatcct atctcgatca catctcctat ttccatttga gaagcgatct
     8221 ttgtcagacc agttggttca tatactgcgg ccgaacctgt aacgccgcca gaatcctcaa
     8281 ttccgaagat tacatgtcct ccttgtatgg tttgcggttt tgtgataacc ttaccgcgta
     8341 cgtaaccagc catgtaaact ctgattgctg ataacttcaa ttcattctgc aaatgcatat
     8401 tagttccttg atttgagcga aagaccatat atccatccaa gatctcttcc ggctgtaacc
     8461 attctaacga tgaaaccaca acttctggac tttcacctct tataccgcaa aaaacgggat
     8521 ctggaccgtg tggagcaatc aacacacgtc tgtggttttg atcatagtta ttgaaggtat
     8581 tcggaaaagt tctcttatca tactgaatta ctctagtcgc atttatttgt ctcgttgtcc
     8641 catagttctt gcactttctg tatgctatag cctcaaacgt gtgatctcca tcttccaaag
     8701 taccaattgc agctagcgag cctactaagc cttgaccgtt tccaaaagtg aaatggcgaa
     8761 cgcaatttgc ttttgctatt ttttccgcct tttgcttact tagaatatca aacattgcaa
     8821 acctaccaaa ttccttgagc gcttccggga ctagtgctcc atttaaaaat gctacaccag
     8881 gatttgcacc catccctatg gctgattctt tttcgataag ttgccttata tcttgaatta
     8941 ttctttcgtg gtttcttgcc ataactctta agcagactgc tccattccca cgggtcttta
     9001 atgggatatt gggatttaac ctaaccaata acggatagtc gaggaacttt gtatttggat
     9061 tctttagtaa atgtcccgta atcttaaatg ctagatgcgt cgtgcatttt ccacatgttg
     9121 agtctgtgtc atcaaatccg atatgaagaa tcatatcctg gcttatagtg caaggatcgg
     9181 gcgttgctgt tgtagtagtt gcacctgcct tcgccatatc gatcatgttg ctactatatg
     9241 aaataaggaa tgaagtccaa atattaattg taactactaa gtttcaggtt aatacaccag
     9301 accacgaaga taaaccgaaa attcatgcat atagagtcac caagaatata tctatgtttt
     9361 tgtcgtttac atgtctgtat aaagtccgca tatgtggtta tatcctaaaa agagcctgta
     9421 cattagtagc catcctattc catgccttct ccttatcttt tggaaacgcc tgttctgaaa
     9481 acctgattgt ttgataaaca tacaatgtca acagaaagta ggaacaagac cgccgactcc
     9541 actagcttca agtgcaaggc atttggtata acatttacaa gagaacagga tagggaataa
     9601 attggaagat gagaaacgtc agcgacttac tggcctgggt tagatcctta ctagtaacta
     9661 aatttagttt aattcattca ttcattcata attgttattt cgtgatgtac ctaagggtcg
     9721 gtctgacttg cgtacatatg cctttcatct aaggccattc ctcaactaaa cggagaatcg
     9781 agtcacattg cctcagatgt ttttcttgga ttggaagtgt acgtggcatt acatgctagg
     9841 ttagattgtt gagagagaat gccgatccta atgccttcga attgctctga cactattact
     9901 aaacaattag tttattccga atcattttta ataatggact agagattgta ttcatgtgaa
     9961 aaagtcaata gcagatatag acttcacgaa acgaaatggt ctaatccctg tggtacttca
    10021 ggatagaaaa acaaaggata tcctaatgct tgcctatact aatagagaag cccttgataa
    10081 taccttaaaa acaggaaacg cttggttttg gagcacctcc cgcagtagat tatggatgaa
    10141 aggcgaagag tcgggaaata tccagcctgt taaggaaata cgcgtggatt gtgacgctga
    10201 tgctttgcta tatgtggtag attcagaagc ccctgtgtgc catactggca accgctcgtg
    10261 cttccataac aaactagttg atgaaaaaaa ttgagtgaac ttatacaaag ttattaaaat
    10321 ttttatataa aatcggttaa tgacaattat tacgtacaaa atgaaaatat attgataatg
    10381 attatttttt tttgaaaatt cagtattact aaaattgtga ttagccaacc ttatctacta
    10441 catttggaga atgttgatta tcaaaacatg ggcattatac agtcgctaca atgtagagag
    10501 tgcagaaaag aatatgaacc tcaatttaga tatgtatgtg aagagtgctt tggtccactt
    10561 gatgttatgt acaagtttcc ttctagtata aaaaagaaca ccttcgaatc acgagagaaa
    10621 aattactggc gttatttcga actcctgcct atcgctgata aaaacaatat tgtgaatcta
    10681 aatgcaggtt ttactccgtt acaacaagct gataggcttg gcacacatct aggaggaatg
    10741 aagaatttat ttattaaaaa tgattccgta aatccaacat tttcatttaa ggataggcca
    10801 gcaggagtag ccgtttctag ggccaaagaa actaaattaa aagcagtagg ctgcgcgtct
    10861 actggtaacc tagcttctgc gactgctgct cacgctgcta aggcagcatt gccgtgctac
    10921 gtttttgccc catccgatat tgaaaacata aagatcgcac aagcattatc atatggagct
    10981 gaatatgtag cagtggatgg aacttatgac gatgctaaca gggttgcctc cgtaatcggc
    11041 gactcgaaag gtatcggaat agtcaatata aatatgaggc catattacgt ggaaggttca
    11101 aagactctcg cctatgaggt tgctgaacag ctgaattggc aggttccaga taatcttatc
    11161 attcctgttg gaagtggagc aatgttaaat tcgatttgca agggatttga agagttgcac
    11221 aacctcggat taattgatag catagcaaat ctaaagatca cggctgccca accacatggc
    11281 tgcgctccaa tagtcgatgc cttcaagaaa aaaagtgatg aagttatacc agttgaaaga
    11341 cctgacacca ttgcaaaaag tcttgcgata ggcgatcccg gagatggatt gtatgtactc
    11401 agaagattaa ggcagtataa cggaattgca gaagaggcta ctaatgacga aattattgat
    11461 gggatactaa tgttggccaa gacagaaggc gtgtttacag aacctgctgg tggagtagcg
    11521 gtagcggtac ttcaaaagat ggttgaagaa ggtaaaatcg acaaggatga aaaagtagtt
    11581 tgctatatta caggaagtgg gttaaaagca acagaagcga taacagctgt gcttccaaaa
    11641 cttagcgctg taaagccgga cgtgcaaaca gttttagcaa tgattagata attacatgat
    11701 gaatctgcat tggttgacat aaacattcaa gatggctaat atcgaattca tcattccatc
    11761 agtcctcaat aaaggaacgg gggagaaaaa aatttcagta gaagctcatg acctacatga
    11821 agcatttgcc aaggtctctg atattttggg agacgatttt aaacgtaggg tatttgatct
    11881 gaatggtaag ccacgtgccc tgatcaatat ttacattaac ggcaggaata tgcgctttag
    11941 cagtgatggc atgtcaacac tactcaaaga cggggactcc ttgtatatat tgccagcagt
    12001 ggccggggga gcggagctga cgaacgaaga tatgcaacgc tattccaggc agataatgct
    12061 cgaagaaata ggattcgttg gaatggaaaa gctgcgcgat gctaaagttt gtgtggtcgg
    12121 gatcggaggg atcggaaacc ctgtcgtaac tcaattagca gcaatgggtg ttggcaaact
    12181 gaaaatagtt gatagagatg ttgttgaaat ttcaaactta catcgacaac atctttacaa
    12241 tgaagacgac ataggtcaag tcaaggttga agtcgccgca gagcgtctga aaaaaataaa
    12301 tcctcatgtt gaaatcgagg caattcctct ttccattacc aaatacacgg cagaaagcat
    12361 cgtcaaagga atggatatca taattgatgc acttgatagt gttgatgcga gatatgctct
    12421 taatgatgca tgtatcaagt acaatatccc gtttatctac gccggagcac tgggaatgtt
    12481 aggttcagtc tgtactattt taccgaataa atctgcatgt ttacggtgta tgttccccgc
    12541 attagctgag gaagacatgc ctacatgtag taccgaagga gtacatccat cgatacttta
    12601 tttggtggcc ggcgttcaag tgtcggaggc agtcaagatt gttacaggcc aacatcctac
    12661 acttgtaaac aagttgctct atatagactt gaacgaactg tcatttgaca agatccagat
    12721 gtttagacat gaggaatgct cttcatgtgg accaaaaaaa gagagtaaga aagaagttgt
    12781 tgtacaacag gaagaactga taattgaaga gctctgtggt agagatagag gcaagagaac
    12841 atatactgtt agtccatcgt caacacttcc tgcatcgatc aatctgaccg gaatagtgaa
    12901 gaatgccgaa tcacagggtt atgatgttaa agtgagaggt aatttgggga taactgcaat
    12961 aagtacaaat cctaacaaat tatcaatcag ttttctcaca agcggcgctg ctacaatagt
    13021 cggggccaaa gacgaagagg atgcattgtt gatatacaga acttttgcga aggaaataaa
    13081 atgatctcag gcgcctttat gcaaaatttg atggttacga tattaaaaac cgagacttaa
    13141 tttaaattag gatacgtaag gaaaaaatat tatcttttcc ttaactgtgg aagaagtagg
    13201 gttagccaca tctgatattg gtcggcaatg tcagatcacg taataacgtg caatatgaaa
    13261 atccaagaaa tgtcaattgg gtattctctc atcttagtaa taggtggtca gtggtagttt
    13321 gcagtagcgt tagtagttgc attttaggac gagtcggttg atactccgag tatagcagct
    13381 acctttttgg tttcatctgt agtagataac tgaacataca attcctctgg tctgattacg
    13441 tattcctctc ccagtctgtt atccatttca tgtctaagaa ttaactcacc cttaattgga
    13501 cttttttctc ttaactttat tagtaatggg gcgaagcctg ttgcgagtga ttccagaaag
    13561 gagattacta gctcctcatc ataaactaac aatggagaag atgacgataa agatgaggcg
    13621 gtaatagagt taaccgatat cgcatttctg tcatctactc taattgtagt aagcttagac
    13681 gtggctaatt taggatccga aagattcctt attttgtctg ctaacagctt accaagaaaa
    13741 aggatttttg aaatatccgt aatttgttga atttcttttt gaaggagatt taatgtacat
    13801 ctttcttcgt tctcaacata tacgacgacg gttggtagtt tgccttcaga tattagcttg
    13861 ttataaaatt cagcatttgt gacaaaatcc tcatattcaa aataagacaa gccataatcg
    13921 atccttatca ctttgttact tagttttgta ggatcatccg caagcgttat cgataatata
    13981 ttctcaccct ctaactttgc ctggacgagg taataattgt cgcacgaaat gtattcaggt
    14041 tctctatcgg tacgagcccc gttatccgca ttcttactac cgccactact actatttcca
    14101 gaaacaaacc taaacgacct cttaaatttt tcagacatag gagtcttgaa accaactggt
    14161 atgagcagat tatctattcc tagatctgaa acttttcgcc tatgactcca aaattctata
    14221 gcggagcaat caattaagaa agaatatgag acgcaagagg gaaaaacagc ggcgctgtta
    14281 ggaatgtctc catgcgatag gactctaagt gtggtcgtgc gggaagtgtt gtacaggctt
    14341 tcgtcttcat tatcaattga aatcattaag acattcaggg cgtctgaaaa tagcgtgtaa
    14401 ggtaaattat cgagatcctt taataaccat gtttgtaaaa gaataattgc attcttgtag
    14461 gagcctatta tctttgattg aatataatgc ttatacttct caaagtatcc agagtattcg
    14521 gttttggtat ttgcaagagg gcgcattaac gcattctttg attggaaaaa aagatctgca
    14581 tctgcatggg ttggaaacat agtggttatt atgtcttcat gatatttgtt ttgaaaacca
    14641 tccaacaatg tttcagttga ggttttaagc cgctcaatgt ttgtcatcat ctcgttctgt
    14701 aaagagttcg taatttctga atacaaggtg tagccttgca acattattat gaatatgaat
    14761 aatattgctc gagacagaca cgaagtcttt aagcatatta ataaaattct tgttttcgtc
    14821 aaatggcgag taatcgccaa aaaggtacct tccctctttt tcgttaaaaa tatgatgagg
    14881 attgcaaata tcctctctgt caatacctct tgtcatataa tataatataa tataattcgt
    14941 tttaagtata tgaattaaag ctgttagtat ctttgaagtc acgtcatatt attaatttat
    15001 tttttattgt agaatcaact aatattaacc gaagtatcga agtacattaa ataggcgacg
    15061 aggtaattgg taaacaaggg gtagcggaga tgggttcata gcggtacgct ttttaatatt
    15121 attattgtat tgcttaacaa actgccatcc cattaaatag tccagctgaa agtcaagtac
    15181 tgtcttgttt agtctatatt ctattttttc ttttctctgt aatcgcagat cagtagtttg
    15241 ctctgtttta ataaatataa aaatttaata gaatagtacg ataataaagg gaatctatct
    15301 tggacgaagg gacgacctgt cttttaacac gattcttacc attttttacc cgcaatttca
    15361 cgcatgtagt agaagatata ttgctgtgca tatccagaat atttgccaaa ataattcctg
    15421 atagtcgctg aaaggacttt atattgattt atagttattc tttcagtcat tttgaacttg
    15481 ttttcatttt cattgaagag ccaactataa tatcgagaga gcgccctcaa aatccagacg
    15541 tcaataggga aggcatcgag tttctccagc gagaataata ggatgcaatc agcaatttta
    15601 ttgccaatgc catatccttt taagagttct tcttttgcgt catgatagct aatacgaacc
    15661 aagtaatcta tatcgagatt gcctgagacg atatggtttg cgagtgcttt aatagccttg
    15721 gctctatatc ccacaccgca cgaatgcaac tcgttaatgg ttgctttatt tagactgttg
    15781 acagaaggaa acgtgtaaaa ttcttttccg tcaaatatta ctttacaacc aaattttttt
    15841 gaaaggttct tgagcatttt acggatcatc aaaatgttag tattactggc acatacaaag
    15901 gaaaaaatgc attgataagg ttcttgtcgc atcaaacgga ggccaggata ttttcttatt
    15961 gtcttagaga ccagagtatc atttgaaaga gtcgataata ttttctcaat gtcgtcgtcg
    16021 agtctaaaga cttttctctc ccatcccttg acctcaggat aggaatagaa ttggatcccg
    16081 ttatttttgt tattattaat acagctactc ttgtttgtcc atgaggtaga aaatttaagg
    16141 atgtaatctc catatgtgcc ataccaagaa ttacctattt gctcccagag aaacatttgg
    16201 ccgctgttaa tgctgatctc tgggttaaaa ggtatggtca agattaatgt attgtcaacc
    16261 ttattgaact ttgttacttt cttcgtcttt tatttttttt aagtccttct gtaagatttc
    16321 aattgtcttt gggatctctt tttcagcaca gagaagacat acctgctgta aagataaggg
    16381 atatgtaaag tactttgtat ttagtgggat atcattctta catgatacac atttcttcat
    16441 cttgtacgga tcagtatttt tgattatcaa ttgtgataaa aaaaatcggg taatttataa
    16501 atatgatccc aaaatatgct tattaagagt agcatattgc cgccagcaag ttgagttgga
    16561 agaatctgtt ctcattacca cttcatcatg gaaagaataa taataatttt agtgtttgca
    16621 aaaaagtaag aaaacttttg ggagaataat gcagcatagt ttggaatgga gagttacttc
    16681 attatatcga cagacgatgt taaaaaagca gagtaatgaa atgacaccgg caaggcctag
    16741 taccaactca tatttcaatt gattctcctg tatccggagc ttttgattcc aatccatatt
    16801 tgattttgat ttcttctgca aacttagtac atgaaggtcc atcgccatgg acggtaagca
    16861 cctttgggtt gccttttata tgttcaagaa tttcgaataa ttcgctccta ctattatgtc
    16921 cggaaaattc gaaccgtttc acatcagcaa gacattttct ttctctacca tcaaaactta
    16981 ccactctccg atcgagaagt gttcgaccag gtgtgccttc gccctgatat gataccattg
    17041 caacaccatt tttatcactc ttggctattt cttgcagata gaacactgca gagcctccaa
    17101 ccaacatccc tgcaggagag atgataacgc agggctcctt aacaacactc cttcttctat
    17161 tccatccact tatccattcc gcttcattga ttgctcgctt gaatatttcg gcatctttaa
    17221 ggaaggttgg atgtttcatc ataatttgat ttgctttaag agccattcca tccattacaa
    17281 ctttgtgtcg aaaattgtta gattttagaa cggttgcaat ttcctgagct ctttctacag
    17341 aaaacgcggg gataaacagt gttccacctc tctcaagtac atcataggcg aactccagta
    17401 actctttttc agcctgatct cgtggcatct gctcactctg agaataagta ctctctatga
    17461 tcattagatc aatctcacca aagtccaaat cggcaggtct gagtaatttt gagcctcttg
    17521 tattaatatc tcctgtatag aatatccgtt taccattatg ttctaaaata accgaagcac
    17581 cgcccaacac atggccagac tcatgcagag ttactttgac atcgtttacc atatatgttt
    17641 ccttgtattg caaattcttg gaatttttca tcatcgttat tacgtcaata tattcaaaag
    17701 gtaggtagaa ccctgatatt tttatcatgt cttcgattaa taatcttgac agctcaaatg
    17761 taggaggcgt acccagtgct tgtactgatg ctgattcaga caagaacagg gatgggacaa
    17821 atccggaatg atctagatga gcatgagtaa tcactacagc atcaacatct ttcggcctga
    17881 catgcatagg gaagattggt tctcttttca agagtacccc ataatccatt aaaatgcctg
    17941 tactattcgc atttacaagg aacgctgaac ggccgacctc tttggcagcg cccaagactg
    18001 atactttcaa tttatccttt attctaaggt aagtgcttta agtctttcac agtgaggaca
    18061 atgacgacga tattattgtc ctcatcacca gggtgttcta gtaatgccta tgagattgca
    18121 agcagttagt gtagtttaat agataaaaag aagaatttga acagatttac atgaacttcg
    18181 gcagggtagt gccttcactt atatttttca aaggcgtaga taacaagtcg gtggttgtag
    18241 ccccaaaaat gattgagaaa tttagatttt tcaatactga gaatgctaat aaaataaaag
    18301 cattaatagt gtatttcact agggatagca agcattggga aaaaaagaac ttactgctat
    18361 cttttcatca gacccagata gatattacaa ggtttcgtta tttgacagat taggcttcaa
    18421 gcgtcaacga tgcaataatt gcggtaaatt tttttggtcc ctgaatgaga agcaggtctg
    18481 tccagaccac cagtactacg gttttatagg tgaccctcct acaaataaaa gacttgatta
    18541 catcaacgct tggaaagaaa tagaagaata ctttcaatcc aacgcacact cgataatcag
    18601 acgatatcct gtagtctgca ggtggcgcga tgatatctat tttacaatag catccattgt
    18661 cgatttccaa agagtaatcg gggacaaagt tatttttgag ctgcctacaa atccacttat
    18721 tgttccgcaa atgtgtttgc gctttaacga tatagaaaat gtgggtttga gtggcagaca
    18781 ttacacaagc ttctgtatga tcggccaggt ttgcaatgcg gacgcacagg gtggctattg
    18841 gaaagatagg tgcattgaac ttgattatag aatgcttaca aacgccctgg ggataagaaa
    18901 agaagagatc acttttgtag aagatgtatg gatgggtgcg ggtgcctttg gttactctct
    18961 ggaatatttt gtaagtggac ttgaactagg caatgctgtt tttaccgaat ttgaggggaa
    19021 cgagaacgac tatagagtaa tgaccaataa gattatcgat atgggagctg gacttgaacg
    19081 actctcatgg ataacaatgg gaacaccgac aagttacgat tgttcttttg gtcccgtggt
    19141 gcgaaaatta gttgacaatt ctggcacctc tgaaagtcca gaaattctat ccaaatattt
    19201 tacagcagtt tctactaaac ttgactatat gagtggagac attcacgcac taaagtcact
    19261 cattgctaag gaactaaaaa tctccgatga cctacttacg aaaatgacta ccccctacga
    19321 ggcaatttat actatcgcag atcacactcg tacactggtg ttcgccatct ctgatggtgc
    19381 actcccatcc aacgtgggag gaggctataa tctaagagtg attctacgta gggcactttc
    19441 gattttggaa agattaggtt ggagcatgaa actcgaagat attgctgata tgcatattga
    19501 ttatgtcaag cagatgtatc cagaacttga ggaacacaga gaagatgtca ggacaattct
    19561 tcagattgaa tctggtaggt atataggatc aagggaaagg atggaagcga ttgctaattc
    19621 aataaaaagt actgagaaga agctgatggt ggatgatctt attagaatgt acgaatctga
    19681 cggtgttact ccagactttt tggtcgattt ggaagcaata gacagtatcc cgcctacttt
    19741 ttacacaaag ctcgctgaac ttcatactag ccaagcagca gctcaagctc aaaagccaat
    19801 ctacggctta gatggtgttg cgccgaccag gctattatac tacgaagaca agtcaatccg
    19861 tgaattcagt gccaagattc ttaaagtgat cgataaaaaa tttatcatac tagaccagac
    19921 tgcgttctat ccgcggggtg gtggacaaga accagacacg ggagagattg acggcgtaaa
    19981 agtacttgaa gttataaaac aagcggatat cgtagttcat aaactacaag gtcccttaga
    20041 ttttacagac ggcaaaaccg tgcatggaac agtaaatgac cgaagacgcg aattaatcac
    20101 caaacatcat actgcaactc acgtgcttaa tacgtcggcg agatttaacc ttggatcgtg
    20161 ggtatggcag aattctgcat tcaaagagga aaattatgcc agacttgatg ttactcacca
    20221 ttcctctctc aacagggagg aggttgaaag aattgaaaag actgcaaaca atgtaattag
    20281 acaaaattta ccagttaaga tcaagatcta tgaccgtgtt gatgcagagc aggactacag
    20341 ttttagaata tatcagggtg gtgttgtccc aactagcaat atcagaattg taaatataga
    20401 aaactgggat atcgaagcct gtggtgggac acatgtcagt acaactggtg aaataggact
    20461 tatcaaaatt accaaatctg aaaggattca ggatggagtt gtcagattag agtttgttgc
    20521 tggggaggcc gctatgaact atatccaaaa gcaagaaaac cagcttacga tgatcgctca
    20581 gtctttaggc tccagtaagg aaaaggttgt cgagtctctt caaaagagta tagttgaggc
    20641 tgaggcagca aaaaagaagg taaaaactat gcttcgaaaa attactccct caatagcaaa
    20701 atctgtctct gaggaggcaa agcaattatc ttcagatggc atcaagtttt atcatgtacg
    20761 tgataatgaa atggatgagg aatatcatat ttctgtagga gagaaatcta ttgaaagtga
    20821 tccatctctg atctatgtgg cgtttatatc aaaaggtgaa ggaatccgtg taattgtatt
    20881 tgttggcgaa caagcacaga aaaaaataaa agctgccagc atcgcaaggc aaatttctgt
    20941 tcaattagga ggttcaggtg gtggcgatgg tagatttgga cagggcggag gtagatttaa
    21001 ggacaagata agcactgctt tggtttcagt agaagaattg gtcctgagaa gtagtatgta
    21061 gtataataat aaaagtaatc gaaccatgac cagtatcgat aataaaaata atggggacaa
    21121 taagaagaat ggagagagga acagaataga ttggaagtca gttgaagaaa agtggcgaga
    21181 caggtgggaa gagcaaagga tcttccaagc agatccggat tttggaaaga agaaatattt
    21241 cataaccgta gcatatccct atccaaactc tcctcaacat attgggcatg gaagaacata
    21301 cactcttgct gacgtacatg cccgttacat gaggatgaaa ggttacaatg tcttgttccc
    21361 aatgggtttc cattacacag gtacgcctat tcttgccatg tcgcgtagag ttgcatctcg
    21421 ggataaagac ctgttggaaa ccttcgataa ggtttaccac gttccaaacg acacaattac
    21481 aagttttgtc gaacctgtaa caatcgcgag ctactttcac aatgagatca ggctaggaat
    21541 gaaagagttg ggattttcga tagattggag gcgcgagttt agtaccgtag acatgatata
    21601 ttcaaagttt atctcttggc agtttaggac attgcggaaa aagggactaa tagtacaagg
    21661 ctcgcatcct gtcggttggt gcccaaacga tcaaaatcct gttagtcaac acgataccat
    21721 aggcgatgtt gaacctgcct tcaatgaata tactctgata aagttcaggc tgggtgatgg
    21781 gtacataatt ccgacggcaa ctttgagacc agaaactatc tatggcgcta ctaatctatg
    21841 ggttaacccc aatatagaat acgtcaaaat atgtctcaca gacaaggacg aaagatggat
    21901 cgtaagcaag gaagcagcta aaaaattaga atttctaaat cataaagtca caatcgagtc
    21961 ttacgtgtta ggaaaagaac tagttgggac attggctaaa aacccagtta ctcacgtttc
    22021 gttaccactc tatcctgccc tatttgttga agccgataac ggaacagggt tcgtaatgtc
    22081 tgttccagct catgcaccat atgattatcg ggcacttgaa gatctgaaaa ataatgtttc
    22141 acttcaaaag gaatttggat taagatctgt tgaagtaaaa cctattgcag taattgaatc
    22201 cgatgactac tctggtatag acgtaatacg ttctaagcag atcataaaca aattcaacat
    22261 acaggatcag acagatactg aaatcgaaaa agctactaac gaactctact cacatgaatt
    22321 ttacaaagga aagatgcttg aaaatacagg aaagtatggt ggtatgcctg tttctgaagc
    22381 aaaggacaag gtaaaaagtg aaattctcat gagtggtgat gcggatataa tgttcgaatt
    22441 gataaataaa cctgtcagat gcaggtgcgg tacacgatgt gttgttaaac ttttaaatga
    22501 tcaatggttc ctgaattatg gggatacgaa atggaagcat ttagcacatc aatgcgttga
    22561 caacatggaa atagttccag aagagattag gcaggaattt aactacgtac tagattggtt
    22621 aagagagagg gcttgtgcac gaaaaaccgg gttgggtact aagttaccct gggatcagga
    22681 ctggattatt gaaagcctct cagattctgt tatctatatg gcatactaca tattggcaaa
    22741 atatgttaat gataaatcaa tcattgatgt taataatcta aaggattcag tgtttgatta
    22801 tgtcttacta gggagaggca acattgtgga ggtcgcttca gagtgcaata tctctacagc
    22861 attacttgag cagataaggc aagaatttac ctacttttat cccgtggatt caagacattc
    22921 tggaagggat ctggttccaa accatctgtc attttttatt ttcagccatg tagcaatttt
    22981 tgacagagag aattggccaa agcagattgt tgtaaatggc tccgttttga tggacggcaa
    23041 gaagatgtca aaatcgcttg gaaatattat tccacttcgt gatgccatca aagaacatag
    23101 tgcagatgca atccgtttag caatgcttat ttctgcagaa ctcttacaag atgccgattt
    23161 ttcattcgat acggtaaaag gaattcggtc caagttacaa gatatatatg acatgggaat
    23221 agaatcttct acaaactccc cacaatcaca aataaaaaga gcaaaaataa atgaagaaca
    23281 agtagatcgg tggctactca gcaggttaca gcgcaccgtt gcagatacta cgatgtcaat
    23341 ggataagtta cgtgtgagag aagcggccca taacatcata tactctctag atcatgatct
    23401 acaatggtat caaaagagag taaaagccaa gagtcgcgaa agctcttccg ttcaatatgc
    23461 tctttcaacg tttcttaata cccgtattag gatgctggca ccgtttgccc catttataag
    23521 tgaagaggtt tgggagaaaa ttgataattc atctacttcc atcatttttg ccggttggcc
    23581 atcagctgac gaagataaga tagatatcgt cgctgaagaa tcggaggcgc taattatgaa
    23641 tttgattttt gatcttcaga agattgtcaa agtcacgagg attactccaa aaaaaataat
    23701 tttttatact gcagcacgct ggaaattaaa agtatataat aagattctct cgagcgtatt
    23761 tttggagagt aatcctaact ttggagatat catgaaacag ctgattaaag atccggaaac
    23821 aacaaaggca aaaaatgatt ccaatatggt gcgaaaaatg atcgaagata ttctatccga
    23881 tccaattgaa gcaagaaaca ggcgactacg attcgagaat ttcgatgaaa gacttccaat
    23941 agaggatgct aggaacttat tatctttaga aagtggaaac gagcatgctg agatattggt
    24001 atattcagaa gatgatactg atcagtcaga atacgatcca aagcacaaag caaaatttgc
    24061 tagaccgttc aaacctgcaa tttacttgat cgatcaataa caaatattat tgattgttac
    24121 tcataccact cacctcgtta tccctgttac tacttggcct tttgcctttt cctcgcagta
    24181 ataatagatg aaactaaata taatgaaaac cctagcatga ccatcgggag tccataaagc
    24241 gaaaatccgc ctctatgcgt atagcttccc actcccacaa atatagctcc tactattaac
    24301 aaggctaatc ctgatcgagc cgctgcgggc atctgaattt tcctcttcga agatgatgac
    24361 aatgtatctg tacctccttt cacaactaag aggcactaat atcatttttt tgtcagttaa
    24421 accaatcaca atattataat tatggcactt gagtggattc cattgtcaga tcctagatga
    24481 acgccgggca tatacggggt gcaaatgaga agcacggatt ccctactatt gaagatatat
    24541 tcaaagcaag ggatttgcta aacgaagctg tcagaaaaac accattgcaa agatctgaca
    24601 cgttttctaa attaacaggt acaaacatct ttcttaagct tgaatctttg caaaaaacgg
    24661 gttcgtttaa ggtgcgtgga gcattcgtta aactcgacat gttgtcgaat gaagagcgca
    24721 aatacggcgt cattgctgct tcggcaggca atcatgccca gggagttgca tatgctgcat
    24781 tgaaaaagaa tatttcatgt actatagtga tgccgaagaa tgcgtctccg gccaaggtag
    24841 ctgctacaaa atcatatgga gcgaaggtaa ctctacgtgg gttagattat gaggaatcct
    24901 ggcttgcagc acaggaaata gcaaaggagg agggacgcac ggtcattcat gcttttgatg
    24961 acgctcaaat aatttctggt cagggaacaa tcggtctgga attgttggag gatctccgtg
    25021 aaattgacga agtttatttg cctgtgggtg gtggagggct tgctgcgggt gtcgcaatag
    25081 caataaaagc aaaaaatccc aatgtaaaga ttgtggggat agagtccaaa gcatttcccg
    25141 ctatgaaaga atctctagcc aaaggctctt tgcaacatgt aaaagcagga tataccatag
    25201 cagatggcat ctccgtaaaa tcacctggca aactcactta cgaaatagca agcaaatacc
    25261 tagacgatct aagattagtc gaggattcat cgatagttga aacgatgttt ttgctcatgg
    25321 aaagagcaaa actggttgta gaacctgcgg gggcagcaag cctcgcttat ttaatttcaa
    25381 acggtcaccc aaacaaagat aagaatgtta tagccttgct atctgggggt aacgtggata
    25441 tgtatctgct tggacaagta gtagcaaagg gtcttatgca tatgggaagg atgcttaaaa
    25501 tttttatcca gttaccagat agaccaggag cattgaagac catagtggat agtattgcag
    25561 aactaagtgt taatattgtc gaagtcgacc atgatagatt gagtcccaat atttctgctg
    25621 gaactgcggg tgtatactta agtcttgaaa tggaaaatgc aaatcatgca aaacgcttaa
    25681 tcgactttct agaggcaaaa ggagtcgaat tcagaatcgt gagttgacat aattcctaat
    25741 agcaatctca cagaaagcaa cagccaagta tttagggatt cgtatatgtg ctatatatca
    25801 cgcatgcatg tactaccatg atgacacctt tagttggtaa agttatactc tctaaaccct
    25861 gccttatcag cagtggtgcc acctattttt actaggacga actcgttgtg gacaggcaaa
    25921 agagaggtat cgaaatttct ttcatttccg cgaagtcttt catattcgtt aattgaaatc
    25981 ttttttctgg gtcctatttc ttcctcgagg ttcattccct ctacgatttc cttgtattca
    26041 gactgaatca caccagtaaa taccatcgcg ctactaccac taccgtatga tccaaaccct
    26101 actctcttgc tctcaagatc agtacccttc ttgtattcaa attccaataa gcttctaaaa
    26161 cccatataca ttgacgcagt gtacagattg ccgattatgg tagatgcttc aagtgagctc
    26221 gccattttct tttcaaaaac ctcattgtag aaagatgttt gcataaatgc tcgcctgaat
    26281 ctttcgtcag ctttcataaa ttcagtgtca gccaaaattg attcaatggt gcccctgggg
    26341 tctttgggga ctgtttcttg taatcccatt tcttttatga ctttattcca ccgtgggaga
    26401 tacctccact catgtcttaa caaatatgcc agcgcctttt cacccattcg tctataagga
    26461 agatgaacac tgaataaatc gatgtgatct gttatggatt ccccatcttt cagtcgaatc
    26521 agacctgttc tgatagcttt ttctttataa ctatcaaagg ccttccttac ttgaattaaa
    26581 tataataaat tagagtaggt tccattaact acaggcgtat cctttccaca tggtctgtaa
    26641 aaatcatatt cattactgat aattgtggat gtaacttttt gatcaaaagc tagtaatctg
    26701 ggattctcct ttatcagcat agcaactgca cctgcacctt gtgtatattc cccggcagaa
    26761 ccaatatcat atttagcaat atcactcaca atcactatgg cagctttacc attattttcg
    26821 tctgccctaa tccaatttgt attatcgtaa agcgcatatg atccgcttac acatgcaaat
    26881 ttgcactcta ttccgccggc gtgttcgaag gaactttctc cgtaaacttg ttctagcatt
    26941 cctatcacaa aagaattcat cgctttagat tcgtcaaggc cagactcggt agctacatac
    27001 attctaccta tatcttcagg atgcagatgg tttttttgca ttaatttcaa acaagcgtta
    27061 gcggccatag aagctggatc ttgatttgta tcaaccagag ccatcttctt tactccaata
    27121 ccgtactcta acttgcgagg gtcaattcct cttgcctcag caaaatcctt atagtctata
    27181 tacagctttg ggatatatat agccagatca tcaatcccta ctggcattta tccatctacc
    27241 atatagcatg aacctacaga tatagccaat ttaaagttat atctagctag ttttgactaa
    27301 ttgtcgtaag ctggttgaca cactttgctg ttagtttaat aattttttat agtctgatag
    27361 aagttatccc tctccgcggc ttcaaaacca atttgtttaa tagcatctat aattttatgc
    27421 gcagttagtt cagtcgatct ggccccagta gcggttacta cttcttcacc gatcagagta
    27481 ccaccaaaat cgtcagcgcc gtaatttagt gcaagctgag ccatgccaat gccgtttgta
    27541 agccaagagg attgaagatg atctatcaaa ccattaaaga ttattcttga aatggcgatc
    27601 atctttaaca gatttaatcc tccggctgaa tattttataa ggccatctgc ctgtatccga
    27661 gttttgttgg gctcaaaact ccaagggata aatgccataa aacctctagt tttttcttgt
    27721 agctctgcaa ttttcataat atgtcgggcc ctatgttctt cggtttctac cgtgccatac
    27781 atcattgtag cggaggactt tatatctaat tcatgcgcct cttccattat tcgaagccat
    27841 tcgtgacttt tgattttgag aggactgatc tctgctttca catcgtcatt tagaatttct
    27901 gcacccgcac ctggaaggga atccaagcct gcacttttta gtcttgaaag gacttctttg
    27961 gtgctacttt tctctacctt tgcgatagta tcgatttcag acgcagaaag tccatgaatt
    28021 ccgacttccg ggcatcttgt ctttattgct ttgaaggcgt cctcgtaata ttccagtttc
    28081 aaatttggat tatgtcctcc ttgaatcagc acttgggtaa taccgaacat ctctctagac
    28141 actaatacac gcttcacaat ttcatctaaa gataatgtat aagcttcttc gtgtcccatt
    28201 ggacggtaga atgcgcaaaa tttacaataa gttatacaga tattagtata gttcaatata
    28261 atattattaa caaacgtagc agtatttcca tagagtcgct gtctaagtat attacttacc
    28321 atggcaattg ggtaaatgtc gttggatttg aaaagcctta tgcattcatc cagaccaaga
    28381 atatgggagt ctagtgcatt atgcaaaatg tcggctacat cagacttcgt aattaaatcg
    28441 tgtactaggc cgctagtatt ttgcaatatt gacatatctg tgtggaatat caatgctatt
    28501 tattccttac aagggctgtc ctcttctctt ttgctttaaa gctggacgaa gtttagcata
    28561 tgcaacatat attatatctg gacttcatag atagtgctag tttgcttaaa ggtgttggag
    28621 gtagcggtgg agtaggaaca cctgcattgg aaaaggcaat ggcaggccaa gaactatcgt
    28681 ataacgatgg actccagctc atgaatgaag agaacctgtt tttgctaggc gccgctgcag
    28741 acaatattcg caaatatctt aagggaaata gtgttacctt tgttgcttca tattacttaa
    28801 attacaccaa tgtttgtgcc gccagttgcc ctttgtgcgc attttacaga aagggcaacg
    28861 atgcagacgc atttactctg agcaccgagc agatattgac aagagcacga atcgcaataa
    28921 atgaaatggg aagcacggaa ctccatatag taggtggctt tcatccaaag cttggtttgg
    28981 attactacga gaatatgatg agggcgataa aagcagagtt tcctagtgtc gtaattaaag
    29041 cattgacacc ggcagaaatt ttctttatct ccaggctaac tagaaattca gtaaaggaag
    29101 tattgctcag attgaaagag gccggcctag atgccctacc tgggggagga gccgagattt
    29161 ttgatccgga agtgagagag ataattgtaa gaggaaaatg ttctagtgaa gagtggcttg
    29221 atactgcccg tcaagcccat gagctaggat tgaagagtaa ttgcacaatg cttttcggtc
    29281 acgtagagaa gccagagcac attgttgagc atattttaca aatcagagaa cttcataaaa
    29341 agacacgggg ctttaccacc tttattcctt taaagtttag cttagagaat actgagctag
    29401 aaaagaacaa cataatcaag acggaaagtc cttcaacata tgacttgagg gttatcgcag
    29461 tgtcgaggct gttgctagca aatgtactca ataacgtgtc ggtgtattgg gtagcattag
    29521 gtaaaaaatt ggctcaggtc gcgttgtcat atggaggcaa cgatatggtg ggaactgcgt
    29581 tttcggagga aatattcagg gctgctggta aagatacagg tacttctatt caagagttag
    29641 ctagtttgat taaggaaatt aagagagagg ctgttcaaag agatacattc tttaatataa
    29701 ttagaagatt taattgatga ttgattgagg aggcattcga tattgacaac cttgaatgga
    29761 atgatgtata taataccaat tcgaccttgt ggatgtgtgt gttatgtatg tggaattcaa
    29821 acgaaattca attgtgaaat cagcggaagt attcctttct cctgtcccat tttgaacatt
    29881 ttcttgatag ctttttcacc gttagttccc atgtcaacag tgatatcatt tacatacatt
    29941 ttgataaact tcatcataac atgttttggt tgccctctgc tatacctcat agcatagtct
    30001 accgcagcat ttctgtgttc gagtccataa aggattgaag ctttgaaaag cttgttaaac
    30061 cttccaattt cttcaatagt cattgatttg atacttgcga cattgatccc taggggcact
    30121 ggcagcccat tggtttcggt atgccaccag gctcccagat caagaatgtt gtataggtgt
    30181 gacttattat aggtaatctg cgtttcgtga ataatcaaac ctgcatcgac tttatcggac
    30241 agaacagcat tgggaattag ctgaaaattt atttcctgtt cattaaattt tccgagaact
    30301 agatttagta ataaattggc agacgtcatc ttgcctggta ttgcgattgt agattccttt
    30361 aattgagaaa gtgatagctc tttttttgat accactactg gaccgtagtt aaagccaaag
    30421 cttgctccac tacgtaatat agtgtagtcc ttcaaataag cgaacgcatg agctgaaatt
    30481 gcggtgacgt cgagctcatg ttttaaggca tgtttgttaa gagtttcaat atcttctata
    30541 acatgtttga ttttaaaata cggagatttt actttacctg cttcaatacc gtaaaacatg
    30601 aaagcatcat cagcatcagg agtgtgacct aacgttatct ccctcatagt ctaaatcaac
    30661 attggaatac attttacaga gcaatatttt ctttcttttc tgttctgata tgggttacaa
    30721 taaaaactat catactgact ttctttcttt ggattcattt attcatttct atcgttgcgg
    30781 gatatactcc aaacttaata catatgcata tgcgtatcgt cgttcatttc taataagccc
    30841 caaaagatgt gccctctaaa aacattgcat acattcgtag atgtgaggta tgctcacatg
    30901 tcctacaagt gccctacaag ccttatacaa ttcataatat ccatttttag acttaactac
    30961 aatttctata atcctacttc aaatattaat attgtttgag aagatatttc aataatatgt
    31021 tatatcatac gaaatttatt atgattccat cgcaatgggt tctcgaatta taaatctaag
    31081 ggatcgtgac tttaattaaa aaggtagttt tggcacatac ttaatcctta acattaggtt
    31141 ctgaatgggg ctacaaagtc gccagctgtt cacaaagata tctgtgacaa aatgaaccca
    31201 caaaactatt gtagacatgg gtagggcttg taacaatttc gccagttgtg atatcgaggt
    31261 ccgtaacaag gtacaccagt attaacttta gcgatttctc aaccaacttt ttttcgaggc
    31321 taaatttatt ctatgtccta tacatgagtg attggtgtct cactaacacc tcttatgtaa
    31381 tataacccac attttgcatt gctaatcatc gctactgaag tcataaattt tcctttaccg
    31441 tgctatcccg gaatcatttc cctcgcttag cgaatgcgtg ctatttatcg taacaataga
    31501 aagccgtaca actatctcat agtactaggg aacgtttaat agtcctccaa cgaatttttt
    31561 tcgactatat atgccaaaat tcaaatctac tcaggatatt ttgagaatta tcggtaataa
    31621 agaacaaatt cgtaactttg gtgttatcgc tcatgttgac cacggcaaga ctaccatgag
    31681 tgacagtctt ttggctgcat caggtattat ttccccttcg gttgcaggtc aggctctcgc
    31741 actcgactcg atgaaacttg aacaaaaccg acaaatgact attcgcggcg ctaacgttac
    31801 actattctat gaaagtgaag gcaaggaata tgtaattaat atgattgaca cgcccggcca
    31861 cattgacttt acaggtaggg tcacccgagc gctcagagca atcgatggtg tagtagtggt
    31921 ctctgattcc gtagaaggaa ttatgactca gacagaaaca gtaacaagac aagccctcga
    31981 agaacgagta aggcctgttc tttacattaa caaaattgac aggctagtaa aggaattaag
    32041 attagacccg tcggcaatgc aaaaatggct ctcgaatata atcgctgaat tcaatagact
    32101 tgttgatatt tatgctgaaa aagaacttaa agaaaaatgg aaggttagca ttcaagcaaa
    32161 tagcgttgca tttggttctg cgaaagacag atggggtttt aacttcgcga tggcccaaaa
    32221 aaaaggtatc accttcaagg atgtctacga tgcatatact tcagaagatc ctaatcttat
    32281 aaaattgcta gccgaaagag caccattaca tgatgcggtt ctagggatgg ttgtccaaca
    32341 ccatccgcca cctcatgtag cccagagata tagaattccc aagatctggg gtggcgattt
    32401 agaatccgat attggcagat ctcttgtatc gtgcgacgat aaggggcctg ctgttatgat
    32461 ggttaccacg atcaatgtag atcctcaagc aggcagagtt gcaacaggca gactcttttc
    32521 cggaactgtt aaggatggcg acgaaatcta cctgatagac gcaaaaaggc caggaaaaat
    32581 tcaatcagta aatatctaca tgggaaatac tagagagcta gtaagtatat tgcctgcggg
    32641 taatattcct gctctgttag gtctcgatta tgctattgca ggcgaaacaa tttctactgt
    32701 aaaaaatatt ccggcatttg aatcgataaa atacgtctca gaaccggtgg ttacaattgc
    32761 agtagagcca aagcatccaa aggatctgcc gaagcttgtt gaggcgttaa gaagaattac
    32821 ggtggaagat ccaaatctta ttgtcaagat taacgaagaa accggtgaga ctctgatggc
    32881 cggaatggga gtgttgcatc tagaaatcgc gacatctctt ttgcaggaag cgggacttga
    32941 catcactact agtcagccat taatcaatta tagagaaaca attcgtgcaa aagcaggacc
    33001 aatcatgagc aagtcgccca ataagcacaa taagatcttt atgcgtgtgg agccacttgg
    33061 tgaggaaata gtcgaattga ttaggactcg ccatatccga gaggatatgg ataagaagga
    33121 aatggccaag gttcttcgag aaaagggttg gaatgcggat gaagcacgga atgtcgccgc
    33181 agtagatgta agtggcaacg tattacttga tgaaacgaag ggtgtccaat ttatccagga
    33241 atctatggac tcaattaaat caggttttga ggacgtaatt tattctggtc caatagctca
    33301 tgaaaccgta aggggtgtga agtttgtttt acatcacttt gttccccacg aggacccggc
    33361 gcacaggggt ttagcccaat taatgcccgc tactcgaaga gctatgttgg gttcgatgct
    33421 tattgcagat cctgtgcttc tcgagcctat tctgggtata gaggttaagt gtcctcagga
    33481 acagattggc acagtggcag gtattttatc aggaaaaaga gggaagttgt taaatgtgga
    33541 gcaaaagggt ttaatttcga ttatacaggg cgaagtacca gcgtcagaga cgtttgatct
    33601 atctgaggtg atgcggggtg gaacggctgg taaggcattg tggaatacat actttaaggc
    33661 atggcagccc gtcccacaat caatattcag aaccttagtg ggagaaacaa ggaagaggaa
    33721 gggtctaaat ccagaaccac cgagtccaga cgaatttata gacaaggaat gatcttgtct
    33781 agataatatt attttctact ccctccctcc aaacaatgtg caagacaaag cagtgacact
    33841 aaaactcggt gtgccataag tcaaaacgaa atatgctaac tcctattgcc agaagtccag
    33901 cacatatctt tgcggataag tctggctagc catcgaagaa tggttgttgt cgttactgat
    33961 ccgattttgg ctattgttat ttaagcaaat caaaactata ttccaataga gatctaagat
    34021 tagcgttata tgataattca ttactggcgc tagatcctta ataaatataa acaattacgg
    34081 taatgattac ttttgtatga aacttgacgt gccaaggtat acggaaaggt ttggagccat
    34141 tagtcttcac ggtgttcagc gtgtttacga gctggattct ggatttaacg acggtaggcc
    34201 agcatctgaa gccattcgga gtgtgggaaa tgtagagatt tcagaaattg aaaggaaaat
    34261 ggctattgtg atacctatta aaggagaacg cttaaagtta ctcgaggggg tgttgagcgg
    34321 tataccccat gattgtttaa ctattattgt ttctaatagt ccaaggcaac cagtagacag
    34381 gtttaaacta gaaaaagagg cacttgagca attcaatcga tttgttggaa aaaatacatt
    34441 aatcctgcat caaaaagatc ctggattatc tgatgctcta aaagaggttg ggtatactag
    34501 tatatttggt ccagatgata tagtgaggaa cggtaaggca gagggaatgg ttactggaat
    34561 gctgcttgca aagatggcag gaaaggaata tgttggattt atcgacgctg ataattacgt
    34621 tcccggagct gtgaacgaat atgtaaaaat atttgcctcc ggtatcgcaa tgtctaacac
    34681 accatataca atggttcgta tctcatggat ttacaagcca aaagtgtctg agagtggact
    34741 atatttttca aaatggggaa gagtgtctga aataacaaat caatatctta attcattggt
    34801 atcatattac accggctttg aaactgaagt tatgcgcaca ggaaattcag gggaacattg
    34861 catgtctatg aaattagcag agcttttaac ctattcatcc ggtttttctg tagagccgta
    34921 cgaggtagtg aacatactgg aagagtttgg aggaatttta ccaacagaaa atcaagacgc
    34981 tatggataaa ggtatagaag taatgcaggt agaaacgcgg aatccacatt ttcatgagga
    35041 aaagggcgag gatcacttga aggaaatgtt taacggatca cttggtagca tctaccacag
    35101 caagatatgt cccccaagac taagagataa aatcagtgaa gagctacgcg gaagagaaat
    35161 agtacaggaa ggacgagagc cagatttatt aaaaaatatt aactccttca aaaatgtgga
    35221 tatgcatcag ttcgcaaaaa ttctacatga tcgtgcaata agtttcgttc attctagtta
    35281 ataggtaaat gaactaataa aaatagatcc taaaaaactc acagcagaaa aaacaactag
    35341 aaatgcaaaa caatcaacgt cttctttttt cagaaaaaat tgttagcagc tttttgggat
    35401 gaatagttac ataggatcat tacatttgta gccttgccac gatacggcat aaaccttcag
    35461 atcagcgagt ttgacatctg tcgtttatta ggggacagtt aacatcatgt tactgctatt
    35521 cgggtgccaa ttagagactt tatttttttt aaggtataac ctgttcagta aaaatataat
    35581 tgccactagt ctcaccactt ttacgttctc gcagtctgaa tgtctctcgt gcgctcgttt
    35641 gcaaaggcat gccgtcactt cctatttttt tctagcgaca tctctataaa ttgtcctagt
    35701 aaagcccaat aggcttcaag taatatttca cgatatttcc ctgccatact tcattttctc
    35761 aatatacctc tctccatcac ttcagatcaa atattttcga acaactacta ccattttcca
    35821 tttgttaccg attgatccat tgagcaaaag catcaatgta ccacctagga cagttcacag
    35881 ctcaaagcac gtgttgatat caatgttata ccttaactag attaaagcta tctaatccaa
    35941 tgcagacttt gaagacgatg tattcttcca cgtgaccagc cttgtgttat aatatgcact
    36001 tatctcttgc tgagtccaag tgcaaaaaag aatagaaccg atgaccctcg ttcaatatct
    36061 catcctgtca acagataggc aggtccctac gtaataggta gtgtggtgtt aaaattcaac
    36121 ccagaaaaat atcctttata ttcttacaaa tgtaaccatt tatgttattt ttacttgatt
    36181 aatggtgcta aagttataaa agccataaat tggtatcatt ttatactatt tataactagt
    36241 tgtgaacgag agctccagca gataacatct gcagcaaagg acattccttt gatggatagt
    36301 gacccccagg aagtcgaatt aatcgatcat atgttggact agttgataat ccttctactg
    36361 aagtcctctc actcacaaaa ccttgcgata actaagctga taaataagca ttctgtaaaa
    36421 aggcttttaa gtcgtcgtaa gcaatctatt gtattggagc cgagtcagct gtctatccgt
    36481 agacaacggt tcttgttccg ctaaattggg gatgacaata acatgtgtgg aatagtaggc
    36541 attttgagta aaaagggtga aaacgtcgtg ccgctcatcg gatctatgct ctcttgtatg
    36601 ataaatcggg gtccggacgg agcaggaatc gcggcgaatc gacgtgtagt acagtcggac
    36661 tcgttttcag atttaaaata tcataaaatt tctggagata gcgccttagg ccacgtgaga
    36721 cttgccattg tgggtggtac ttgcggacaa cagcccttta gtagttgtaa tggaagatta
    36781 accatagagc ataatggaga gatatacaat tataaaagaa ttcgaaagag attagtcaaa
    36841 caccataagt ttgccactca aacagacagt gaagttattg ttcatttgtt agaagatcac
    36901 tttagaagaa ataacgatct cctgtgtgcg atcaagaaga ccgttgccga acttgatggg
    36961 gtttacgcta ttgttatcaa agacgaacaa acgggaacta tggctttggt cagagataga
    37021 atgggtgtaa gacagattta ttatggcgaa aatgatcgcc tagtcgcctt cgcatctgag
    37081 cgtaaggcct tgtggaaaat aggaataagg gaaccaacaa acagagtcct gccaggatgc
    37141 gcagtaatta ttaaaccaaa tgataagtta aaagaaatcc aagtatcaca tcctcctgta
    37201 cgttcagcga ggattgtcta taaaacactt acttcggcag ccgaggcata cgaaaaagca
    37261 ttgctcgcat cgatgaagaa gcgcactcaa gattttaata gagttgggat aattttctcg
    37321 ggcggaattg acagtgtaat tattgcatgg ttagcgaaga aaatggtcaa ggaggttatt
    37381 tgttacacgg gcggagttca tggctcaagc gatattgtat tcgctcgaca aatagccaag
    37441 aaattagatc ttaagttgag agttaatgaa ctgacaaacg atgaagtaga acaattgata
    37501 ccagagatta tcgacactat tgaagataca aacgctggac agatagaggt cgcaatccca
    37561 gtctatgctg ccgtaaagct tgcacacgag gatggaatca acgtaatgct cacaggtcaa
    37621 ggagcagatg agctttttgg cggctatgca tggtatgcaa aagtacttga aaaagaagga
    37681 taccaaaaat tacgggagca catgacagaa gatttgctct tgctttacag agaaactcta
    37741 gaacgagagg acaaaattac gatggcacat agtatagaga tgcgcgagcc atttcttgac
    37801 atgaaagtaa tcagactctc aatgcagatg gatttgaaat tgaatgtgaa gggcgtagaa
    37861 gatatgtttg gcaaacatgt tcatcgaaaa ttagctcaga ggttgggtat ccctagaaat
    37921 atcgcatacc gtataaagga agcggcacag cacggttcag gtatgcataa cgtagtcgat
    37981 gcaattgcta gaaaacacgg atttgatgag tctactatct ctaaccggta tcttgatgtt
    38041 cttagagcgc gtgaaaaaat aggaagctca caaagatatg gatatctatt tgaaaatgaa
    38101 aaaatgtgga ttgcagaacc tcatgtgcaa atgtatttcg atagcatttc gaagacacta
    38161 ccaacattcg aacaaagcgt cctggcaaaa ccaaataaaa gatagtgtct aatgatcacg
    38221 ttgcaggttc taccctcgat ccggctattt tgcaggtcga tcttttggaa atccacgtgt
    38281 aataagacaa ggcgtaatta tcaaagatga taacaaaatt tgaactggtg cgagtagtga
    38341 aacagtcgct catgcaacga atctagaaat gttgaaaacg aactacctgc gcatgtaaag
    38401 catcctatta ataattcacc tgcctctcca acgcttccaa taagggatct gctcttggtg
    38461 cttttgattt tttgcactca agttatgaac tcggcggtat gagaaacata cccaatgaag
    38521 aataactatt tcagctgtga tttgacaggg cctttggaat gctcaggtag cagttgactt
    38581 gaccaaatcc ttaaaaatat ggcggcgatt ctatgcgggg taatgagagg aaaaagacag
    38641 aaggaaaaga ctactaactg tttgcgtgtt ttttaatgta ctgttcatat gattctatac
    38701 attttttttg tgtttctgat tgaaccgatc caggtcgaag cttcctgatc ctcatgatag
    38761 ctttttcggc acctaaattt tcttcttctt tgagcagata agcagctaaa attgtccccg
    38821 ttcttccttt acctgcggcg cagtgaacca taacaggttt ttcactatct atttgctttt
    38881 ttatataatc gaccgtaata tccaattgtt caagagaggg agcaccgtaa tcttctactt
    38941 ttaaatggag atactcaagg ttacttccat tattattaat ataatgatta atattatttg
    39001 taagccagtg cgctggcaac ggcacttctc ttacagtaat tatggtcttg atcccatgtg
    39061 ctagtaccca tagaaactgg gaataagtca tgggtatccc actacccgct agcttatctt
    39121 tgataaccca gctgtaattg gttggtctag ttgtcatatg cgcatgtacc catctatagg
    39181 cattgccaat tctagtcata ccgtaactgt ctgattaaat tagattattt aatttatata
    39241 atatgatgat aaatcgtgtc tttctgtggt cagattgggg ctgcaaaaat aacacacctc
    39301 aattacttaa attcacgtat atttacattc tccagctctc tatttcttct cagtttcaaa
    39361 gccaccattt tttctctaca aacggtcata tcaataccaa agcctctgaa catctaatta
    39421 tttaatgaat atttacttta aaggtcaaca gaataagaag gcttgtatta tatctcgaag
    39481 ctttggatta aactatcgga attagtttag ggatggtgta tgtgtcaaaa aattctacat
    39541 gtggtcagca gcactgaaaa cccagtagtg gtcattaaga agtttaattt tgtggaaatg
    39601 agagcgcgaa aacaggatcc ctatttgggt tacccagaga atcgatcgaa ttactggaag
    39661 tatccccgtt tgctctttat aacagtggta atacgcaggc tactgccaaa tttgcatatc
    39721 ccattgcaat taaatcaaat actcacaacc cttaattgtt atcaatgtta tatttcactg
    39781 tataacatct gtctcttata cacatctccg agcccacg
//
LOCUS       DAOR01000129           25806 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4613, whole genome shotgun sequence.
ACCESSION   DAOR01000129 DAOR01000000
VERSION     DAOR01000129.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25806)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4613"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1600..1696
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7959 a         4961 c         5296 g         7493 t
ORIGIN      
        1 gtccaaaatt actaaaccga agtgtgaacg acttacttct gaaaaacgtt ttaaggcttc
       61 ctgagaatct gaaaatgtct ccacattata tccattgttg gtaagtgctc catgaaaact
      121 atacagtaca tcttgttcat catctactaa cattatagta taaccagaat aagaggaaga
      181 agttgtagaa ataggagaag cagaaaaagg aaccactaca ggactgtagt tattttttgg
      241 attttttaaa aagaaccgaa ctagatctat acgacagact actctaattt aaggctgctt
      301 cgatttatta attcatacac cttgttagac taccttaata tcccacctcg caagatcatg
      361 ttcgaccggt tggttggctt gcaacggaac acgtatcacc tctactgtta tagtatgatg
      421 tccgatcggc aacggcttga acaacagctc atagctctcg tccatcgcct gtccacaaca
      481 aggtagttta atgccgaaga cattatctga agagcgtatg gtgtacatgt aaggctgaga
      541 ggtgctttca cggacaatgt tcgatgaaac atccgtgccg tcagccttca cctgcatcaa
      601 cttaatgact ttgttcgagt cttgtgcgaa ggttagaagt tgaggaatcg actcgcctgg
      661 aaattcacct tgagaacact caccttcata cacactcaag tacaatagct gagaagatga
      721 tatctgacat gcttgatgcg tcgcattcac gttagcacca cccacggtcg cagaggccgg
      781 atctcccaaa aaaactacgg atcggttacc tacggttacg tctgccttaa gacatattgg
      841 ccaagtgcta gcgtatctgc cgggctgatt gccccgaaaa tcccaccact ttgctactaa
      901 gggttggaat gatttgatcc cagctggcta atggcaaagc catggtccaa ggtgcattaa
      961 ctcttcccat ttcctctccc agttattaca tatcgctggt attgcgagta tttgctggcc
     1021 ctgctccaat agtctttgtt gtgctggttg ttggggcggt tggttgtatg ctaattgtgg
     1081 ttgtaattga ggttgttgta catactgctc tagtcgttct tcattcaaac ttcaaatcag
     1141 ttagttatct gtacactact tgcaaccaca tgtgggctgc cctcccggga gggcggcagc
     1201 gttaattttg ttatttttta ctaaaagttt taaaaagtaa tgtttcctat gagcgtgcga
     1261 atgaggccta ttcagtttta atctgacttt ctaaaatgtt acaatttgac ctccccagat
     1321 gacatgggta ggaataaaaa aagacgatat taaacctgat acgtattctg gaattatcag
     1381 aacatccttt agtgagtagt aacgttggca taattagcat caaaaaccaa gtggaaattc
     1441 aaattcaacc ctcatattct ctcaatagct caatttccgc tcaatcgact gttagattag
     1501 gcagtacttg tataagtacc cgctcgtcag gggagaaact aacagtcttt gtcaatctgt
     1561 acgccaatac tgatcacttt gtggccttcc tcccgggagn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnctgg gttttaatcg aatgaatgat ctcttttcgt cctatgcaga
     1741 cgagattttt cgctcatcgt ttcctattcg gttttaatct attctcaaat tcttcacgag
     1801 taggatagta cggcttacct cgatggtcta aatctatatg ttttaaacgt tcgatatcag
     1861 ctggaaaaga ttggtcgcag tagaggcatt cgaatccaag tacctcggct actcggcttg
     1921 gcgcagaaaa taagaaatta ggcggttccg tgctttcgaa taatttattt tgttcctcag
     1981 ttcacctgat gtattttcaa tatcatttcg tgatattttc gcttctgctt ctacttcctt
     2041 tgccgcgatt aaagacaaac tattctactc tattctaggt gagtgggccg catttaagtg
     2101 tcgttatcaa aaatgggccg ggtcggattc gaaccaacga cccccgccat gtcaaggcgg
     2161 tatcttaacc aggctagacc accggcccac gaaagctcaa gttttctttg gatagcctaa
     2221 atatatagtc gcctataaag tcagattaat gattttcatc gtggtcggcg tctaaataca
     2281 gaaattcgga ttaaaggtac tgcgcagcta aaaagaatgc tttttttatc aatgtaacta
     2341 tggatcctta tcatcgatga tttcttatca ggttctccaa ggtaattctt atgatgttgt
     2401 gcgtggactt tttccatatt ctataggcaa atacagagtt attatcacgt ctccaccata
     2461 ttatggccaa agacactatg gtcaagcttt ggatgagatc ggtcacgaaa aaaacgccga
     2521 gacatatatt aaagaattgg caaaaatttt ttcaatatgc aaagatcttc ttagtgatga
     2581 cgggagtctg tggctcattt gtggtgatac acgtaggaat catggaaagc tcatgatacc
     2641 ccatagacta gcgcttaaac tggttgaaac aggctatata ttcagggatg atatcatttg
     2701 gtacaagaaa aacaatgttt ccagtagctc caaagacaac tttacacaag cttatgaagt
     2761 aattctattt ttgtccaaga acgaaaaatg tttcaccgct atgaacagag taagaaccaa
     2821 gggtaacgaa gcaataatgg gtaggaataa aacaccaccc tcacatttgg ttcaatatga
     2881 acctgtgaat agagacaagg aaaagattgt taaaattctg gcaattatac acaatgcaaa
     2941 atctaagaca ccaattgaag aattgcccac aacaagtgag atatctcttg catatggata
     3001 tgatccagaa aaatactgcc caacatgtta ccgaaaattt aagcgtcatg caacacgtag
     3061 aagaataggg ggccataagc actaccctat ttttgcagta tgtaattcca gtggtaagaa
     3121 tcccggtaat gtgtgggaaa ttgctaatag ggcacattat ggaaacgaac atttcgccat
     3181 atttcctgaa gctttggttt ctcgaattct caaatttgct acagagaagg gtgattgggt
     3241 tcttgatcca tttgcaggac gaggaacgac cggtatagca tgtacctacc ttggaagaaa
     3301 ttttacaggt atagacttgt atgaagagaa tgttgtcaga gcacaaaaaa atattgcgta
     3361 cgcaatgagc gagatctata aattaaatga aaaatgaata cctcagatcc ataaatctaa
     3421 tacggggagc attcttctac aatttagaat gcgtcccaac gatccgtttt cagcatgcga
     3481 aaaagatgga ctctataagg cgatattttc gcgacgcgat gtccgttctc attttattaa
     3541 caaagaaatc cccaatgata tactcgcgaa gattcttaat gctgctcatc atgcaccatc
     3601 ggtagggttt tcacaacctt ggaatttcat tttgataaag gctaacgaaa ttaggcagca
     3661 ggtcaaagat tcctttgtta tagagcgaga gaaatccgtc tctatgctcg atagtgataa
     3721 agtaaagcag aaaagatatc tttcacttaa gcttgaggga ataatggact ctgctatcaa
     3781 tatttgcgtt acgtacgacc ctacaaggtt tggaccattt gtactcggta gaacctcgat
     3841 tgcagaaaca ggcgtatata gtgtgtgttg cgcaattcaa aatctctggc tcgctgctag
     3901 gtctgaagga attggaatcg gttgggtaag tattctcgca aacgaagatc ttcagagaat
     3961 cctatcaatt ccagattttg ttagacctgt ggcatacttg tgtttaggtt acgtaagcga
     4021 attttcagat aaaccggacc ttgaaaaaac cggctggcta ccccggatgg gattggaaga
     4081 ggttatctgc ttcgaacgat ggggtagcca caagactgac tcgtggaaac aattctatga
     4141 aactatcagt aaaatcaact aattcgtacc tttaactatt acaagttcga cagcccatat
     4201 tgtttcatct tcctgtggct taatgctgaa tgtatttttg ctaagcaaat aattcgtacc
     4261 ggctaaaaat tttaatatgc gccgactaaa agcacgactc ttttaatagc actttctgaa
     4321 ataagcattg tcagcataca ataaaaggta atgtgttaca gggaacgtat aagtgttgaa
     4381 attagagcag atatgggggg ggtgtgaaat gtctgtatga atgaacgcca tcgtgattca
     4441 tacatttccg cgaactcatt tagcctacta tataatgatc ttgctcgatg gttatccatc
     4501 acacttgtct ttactggcat gttatctact ggctggttcg gaattcctct tgtgccgcga
     4561 tatctagcat taaaccaggt tgacattgaa gccagtttat tcacattatt cagtgctctt
     4621 ggcagtatct ggggagtaat ggcgtaccag gagttctcta gaactggaga tctagttaaa
     4681 gcagcaagag atctggccag aaagcttgat cctataatag gtgactatga atatcattcc
     4741 tataatcctg aaaccaaaga atatgaaaat ggttacgtcc ctctcggtcc aatagacttt
     4801 tgggacaaag aaacccaggc tccaagctgg cttgacaagg gaaagtactg gcacatttta
     4861 cttggactcc aagtcgccaa gagagaagtg atattacaag ttgtgaagga ccaggatttg
     4921 ccgtttacat ccgggaagat gaacatcgcg gttacataca aggaattgaa tcagggtggt
     4981 ggtgcttctt ttctccgtag tgtatcgcga agaaattatc gttacagggt caagatccct
     5041 caatggtccg taatgaacac cgaacgtgtg agatttgtgg aacagaaaga acgattgagc
     5101 cttgacggcc cgcaattgaa ttatttaatt gctgacctca acaaaaatct cgtacgtact
     5161 gcagatgaaa ttatcgagcg tgaaaaacct aggatacgat atccgtggcc gacgacgaca
     5221 tatggaattt accttatcaa aagtttgccg cttcaagtta tttacagacg agccacacga
     5281 cactttccgg gcctaaaaaa acgtataatc aatgatacaa atgagaacct tgccaatgtg
     5341 caagacgatg aattggctgc cgccgtaata gaattagcga cacagaaagg attatcggat
     5401 gatcgaatag acaatttgcg catacttatg cttttcctta aaaatgcata ttccaaacag
     5461 gggattggag agggctcaag cgaatatcat aatttccatc atagtcttga ggtaagctac
     5521 atgagtttgc agatgatgcc aaaggagttt cgtaagtaca atttcagctc aggagattat
     5581 gaattaattc ttgtagcttc cctcctgcat gattatgatc ctgcgcaagc gtattcttca
     5641 acggaagacg gggacatgaa ggcgccaaaa ggtcctaagg ttacgaggac aattaatgag
     5701 atatgcacaa caaaaattca tgatgcctat tttacaatga atagaattga ttttgaggat
     5761 tattttcgag agtataaacc tgcattatcg ccagctatag aattcgtaac cacccacccc
     5821 gaatatctca agccagaaca aaaaaaaccc attgaaagta ttattgtaga agccttgatc
     5881 tggcgcacag atttcccata tcttaaacaa aaacattctc aagaaaggtt cacgcagctc
     5941 ttagccgaac tcgagaacca aggagaggat ttcgataaaa tccaatcaat tggtgaaatt
     6001 ctatggcttt cagatctgtc tgtaacctat atgagttcag atccgatcag ggcttgggat
     6061 agagttagta atctctatga tgagctatat cttccaagag tcgaggctgt ttctagaact
     6121 gatgcattct tttcagattt tgctgaagtc gactctttca aagaattaat ccgcgatcgt
     6181 cattttcctg atattttcag gcaaagatgg aatatgatct atcagttttt ccatgagggc
     6241 aatccttcga cgcaacttag caggacaatt agcatggcaa taaaattgta tattaaggtt
     6301 aatgtcgaaa ttcgcatgag tcgttgtgag gttttgaaag aaattgctgt gaataattgg
     6361 gcggagtatt ttattgctat tggtaaagat caaaaggagg tactcaaagc aaaatcagag
     6421 tttgctgact tagatcctcc taatgcatct tcgttctggg gagattctca aaaacttctg
     6481 acaaatattg cggacaagtc aatcgataat ttcttgctaa tcttgtctca aaattttgat
     6541 ccccttaata ctatccaaga aaaattatcc gacagatcca tatttgcggt actcccatta
     6601 aaattagtag attcaggtac attgcaaatt cttaccgact tgcaaaagga tactgggcag
     6661 tataaagaac tcataaatat tgtacttgat agtggctttg agatagatct ggtcaaatcg
     6721 acgaaggtat atttcaaaat agacagcaaa tatcctggat tcacacaagg aagaatacct
     6781 caagtcctca tcttccgccc aaagcagaaa ccggcccctc tcgctagcca gaaaaataac
     6841 gaagatcgag gaacaacgat agcctaaagg cagctttcca cgaaaggcaa tacggggcat
     6901 aaaatatttt acaaaaagga gatggcttgt attctacaac atataattag acccctaatc
     6961 tcaatactag tcactctttg gttcacatta aaaaacttga ggtttatggg tttaagtcgt
     7021 tcgggtttag gaatactgta ctaaactttg agaaaggact tgtagcaatc actggtccca
     7081 atgggtctgg aaaaagtaac attttggatg caataatgtt cgctataggc gaaaccagcc
     7141 cgaaagtctt gcgtgtcgat aagttccaat cgttatttca tgacagtcag aacagctcta
     7201 caaggctcat tcgagcgagt ttaaattttg acaattcaga taggggaata ccagtagatt
     7261 ctgattcagt cactgtaaca agagagatgg aaggacaaac aggtgagagc cagtactatc
     7321 ttaataaaaa gagagtgaca aagagcactt tggtagaatt attagaaatt gtgcttgcaa
     7381 cctcaaataa acttaatatc attcagcagg ggatgataac tcgcatttcc gaattaaatt
     7441 ctgaagaccg tagaaaaatt atcgaggata tcatcggact atcatatttt gatgagaaaa
     7501 aaaatgaagc gcttaagcaa ctcgaggaat ctgaccgtcg cctcgacgtt gccatggctc
     7561 gcatcgacga aatacgaaag cgaattgatg gcttggaaga ggaaagaaat gaccaattaa
     7621 gatataaaca cctagagtct gacctcgaaa agttcaaagc ggtaaaggtt tccaataccg
     7681 taaggacaat cagaagcaat ttgaagtcaa aaacggagct tctaaactca aacgtcctac
     7741 gctcaaaaga cctaaccatg cagatagaaa aaaacaattc agaagtagag aagatggagt
     7801 cagaaaagct aaaacttatc caagaagcgg atattgcaac taggactaaa atggaaatag
     7861 gaactagtgt aacacattta gtttacgatt cggaaaggaa aagagcattg attaaggaga
     7921 cagaacagcg attgattcag attgaaaggc gcattgcttc tctcgagata gaaaagcaaa
     7981 atattaacca gaaaatagag aatttacatc tccagataga ggaaaagaaa acccgtgttg
     8041 atgaaaggta tggaaggtta tctattctaa aatctgattt agctgcgatg agttcgcaga
     8101 tagacgaatt gtcaacgagt gccaatagat atgctgaaat cagaggaaaa ctagaaggcc
     8161 ggcacaataa gctacatgaa gttaagaacc atctagacgt ttcaatagct aggttggagg
     8221 aaaaaatgat aatagtgaca ggtagagcca acagccttga ttcaggaaat ctagagttaa
     8281 aaactaatat tgagaaaaac aaccaactcc ataacgatct ttgcatcacc ctagactccg
     8341 agaaggctaa attggatact gcgagcggcc tgattgataa cctaaaaaaa atgaaagcca
     8401 ctttacaaac caatctcaag acatcgacga acctcctgtc aaccgcagac acttttacta
     8461 taaaatacga ggtaaaggct tccaccgcca aaaatgccat gaccgaagat attgccatag
     8521 cggaattgat gaagatgtcg aatgagtttg gaatcgaggg actggtccac aatataataa
     8581 cgtggaacaa aaattacgag agggctgttc tagcggcggg ttcagaatgg atgaaagcat
     8641 ttgtcgtcga cagtgtccaa tcgatgatct cgattgctga atatgctaaa aaaacaaaat
     8701 tgccacgcct taaggtcatt ccattggaga ttgtgcgtaa tgttaaaagg tcttacattc
     8761 ctgatggtga tcctaatata gttggtagcc ttgctgattt cgtaaattct gactacaagg
     8821 aactgccaga tttccttttt ggaaataccg ctcttgtgaa gaatccgact tctgcgtacc
     8881 ttttagctag agagggctgc agagcggtct cggtaggggg agagcttttc gaaccttcag
     8941 tagcatctat gtcagcagat tttgggtcta caatttctga tctaacgcat gcacttcttc
     9001 tcagtaattc tattgacatt ctaagaaatt tgctaactaa attgaggaga ttaattgagg
     9061 agaaagataa tgacctcaga caaattgtac taagaatcga tcgttcagaa tctgaaaaat
     9121 ttgaaataga gagaaaaata gctaatttag gtacacaaat ttctaattta gcgatgctcg
     9181 ttcatgaaga cgaaaaaaaa cttgagcaac ttactttgga agatattacc gttcactcgc
     9241 aatatgaatc cataacactc gagttagatg gatggcgcag acgcttggca ttggtcgggt
     9301 taacgataga gcgtgtttgc caaagaatgg aaggcgtggc cgatgtttct atcagaagcc
     9361 aattaggaca aatgaatgca aaaaggggtc aaattttagg ttctatagat gcagacaatc
     9421 ttgacttaca agacatactt acatcttcac aagtactgaa aaatgagatt ggaatggcag
     9481 tggaacgatt aaaaaccttg aaagaggaaa ctagtgaact agaaactgaa aggaaagaga
     9541 agattgtcca agcggatgaa ttaaaattta atatggcttc tatcgaatcg aggcttcaaa
     9601 tggagagaga ccgagaacag caagtaatag attctgcagg aacttcagtt ggtaagttac
     9661 aagtatacga gcaaaaaata aaggcgctaa aggaaaatga aagaaagctt tccaaagaaa
     9721 atagttccct tgaaaaagat attgcattag tgggtaaaga tatatcggat cttaattccg
     9781 aggaatcatc tctaatcaat aatcttaggt tgctcggatg cgaagatttg ctagaggcgt
     9841 ttgacgttga tacgattata ggagaattaa cagccgagtt tgatacaata aaaactcgaa
     9901 tcaatctact cgccgatgaa gtttacgtag aagtaattga cggatataga ggtatgtcga
     9961 ctaaaagaaa tctgttggaa aaggaacgtc actcgattgt cctctttatc gaagaaatcg
    10021 ttacggaaaa gaagaatgtt ttcatggaag catttgagaa ggttgatagt aacttaagac
    10081 agactttctc cgaagtaaca ggtggaactg cctggttaga agtagagaat aaggaagtcg
    10141 aattttcaga gggcataatg ttaatggttc aattccaagg caaaacagga cgcgagtcca
    10201 ctgctttgtc agggggagag aagacgatgg ctgctataat ttttcttttg gcactccagt
    10261 cacttaaacc gtccccattc tatctaatgg acgaggtcga tgctcatctt gatgctcaaa
    10321 atactgaaag attatctaag gtcttgttag aaagatcgaa ggacaatcag atgcttctgg
    10381 ttactttgaa agatgctacg gtggctcaag caagcatgat atatggtgtc tattcccggg
    10441 acggagtatc tcaagttgta aggtataagc atccgagcca acttcctctc gaagagataa
    10501 gatctgaggc aagtgcatac taatgcaata tgtccttgtt gctaattttc tcggtagtta
    10561 tgggttatgg gacaggcttt gagaacgcgg ccgtaacgtt gaatagagat agtaagaatc
    10621 agcggaacca aggcacatga agggttataa acaataccca aaaattcaat cggataaaag
    10681 atcaacggaa cctcacaaat tcgctaattt aggcaactca tgaagtatca aaaagttaga
    10741 tcggattaca tacttgatcc acttgatata ctaagatttt ggcttgctgc tatacagggt
    10801 aaatgaagca cgatacataa tggttgtctt cctcaccacc taatagggga tcacttttgc
    10861 agatatccat agaataaaga catctatgaa tgaatctaca accggtatga ggtctgtctc
    10921 ttgtctcgcc ctgcttaatg cggatgattt tttccttttc caaattctgt ggttcaggtt
    10981 ccgaaattgc gtctaacaac gcctgcgtat atgggtgaag cggattcagt aagacttgtt
    11041 ctattggtcc tgcttcaact atcttcccag catacataat cccgatctca tcaccgatat
    11101 atctggcagt agacagatcg tgcgtgatat ataggtatga aagtttaagc ctgtctttca
    11161 aatttttcat taactcaagt atttcggccc ttacagaaac atcaagcatt gataccggct
    11221 catcggccaa aattaacttt ggtttgagaa cgagcgatct agctatagat actctctgtc
    11281 gctgacctcc ggaaagcatg tttggaaatt tttcagcaat gtctgtagca ggctctaatc
    11341 gtacctcctc cagagatcct aatacgtcct cgattctttc ttgttttgac ttgttcttat
    11401 tatgaatatt caaaggttcc atgactatat ctaatattct cattcgagga tcgagcgagg
    11461 tataaggatc ttgatgtatc atttgtacat ttgtgcgaaa cttcttcaat ttgttgcctt
    11521 catattttgt aatgtcatct ccatcaaaaa ctatcgaacc gctgtcaagc tcaatcgcac
    11581 gtagaataat cctcgctaaa gtagtcttac cggatcctga ctcgccagca acgacaaatg
    11641 cctttccctt ctctattgtt aaacttacac cgtcgacagc cctgacataa gactggttgt
    11701 tcttacggaa catatccttg atacttctcc taatgggaaa atacttcttg acattgtcta
    11761 actggagaat ttcgttcaag tattcacagc agacttaaca gtgtttgata atataacaat
    11821 tttgaatgca ttgcgttatg cacataatta agtaataatt atataagtac atggaatctc
    11881 caagccatgt gcatattact ggcaaggttc tggtttgtga cccaatagat aatgttggcc
    11941 tttctacctt aaaaaaagca ggaatgactg tggactattg tcctcaaatc gagacaaatg
    12001 atctcatttc tagggtcaaa gattatgaag tcatcgttgt cagaagcaga acaaaaatta
    12061 gtcgagaagt tatagacgca gcaattaacg caaaaattat tgcacgtgta ggcgttggtc
    12121 tcgacaatat agatgttgat agtgccaaag tcaaaggcat acgcgtaatc aacgcgtccg
    12181 aggcagctat taacgcagtg gccgaactcg ctataggcaa catgatagct cttgctcgga
    12241 gcattacacg agcagacagt gaactgaaga aggggaattg gataaagaaa aatttgatag
    12301 gtatagaatt aaggggaaag tatctggggc ttattggtgt aggaaacata ggtcggaatg
    12361 taggacgtat tgcacgcggg ctgcgtatga acgtcattgg atatgacatt tttcctataa
    12421 atcaagatta tatcaaagaa gtaggcttga ttaaaactga tcttaataca ttggtagaga
    12481 gtgctgattt tatttcctgt catgtaccct taacatctga aacgagacac atgttaaatg
    12541 cagaactctt ggcaaagatg aaaccaacaa catacgtcat taacacctcg cgtggtgagg
    12601 tcatcgatga aaatgcgtta taccaagctc taaaggctaa tagaattgca ggcgcggcgt
    12661 tagatgtatt tgagatcgaa ccaccaacga atagattgct tatagagctg tcaaatgtaa
    12721 tatgtacacc tcatataggc tcacagacca aagagtcaca ggaacttgcg tctacagtca
    12781 ttgcagaaaa ggtaatacag atcatgtacg aaaaccaatc tgatttggtg agtagttagt
    12841 cggtaaatat gacgcttagg gtttaagctg cttatagtgt ctcaattggt gaattatgcg
    12901 ttccacttta tcaaaaattg aatatgtacg tctaagcgcg tagatattct gtgcaacgag
    12961 ggatgtcgat cgataattca tcttgtatac agatattaag ggtcatcgaa tcatctacga
    13021 acaatatgcg tggctacatg gctgcaacat ggctcaaaat gtggaagctt aatactgatg
    13081 aactaaagtc gaaagcaatt acatggagaa cggaacatgc tatacaaaga atcgaaagac
    13141 cgagcaggct agatcgagcg cggaaattgg gctacaaagc aaaacaaggt atcattttgg
    13201 tgcgggcaag agtaggcagg ggaggtatga ggaagcaaag accggtttca ggcagaagac
    13261 caaaacacct cggtgttgta cacatcaaac aaggaataag tatgcagcgt gttgctgaac
    13321 gaagagttct cgaaagcttt ccaaacatgg agagtctggg gtcttattac cttcacaaag
    13381 atggccgtta cttttggtac gaagtgatac ttgcagaccc ttatcatcca tctatttccc
    13441 acgataaaga aatgtgccga agacttaaag cgttcgcaaa aatcaaccgt tgaacggaaa
    13501 ttagatcgtg gactcaccgt aacgctctac ttagcgaaga ggataggata agattacgct
    13561 aacggaattt acctaccacc gatactacaa agaatcctag caagaccgaa tacacgagca
    13621 agtttaattg tgctcttctt tgactatctc tcttagtgaa ccgatgattt tgtaaatctg
    13681 ttctgggctc gtatccttat ctagagtgac ataataaaag aaagcttcgt taggcaacgt
    13741 aaggattttg accttttcat tttcagtcaa agtatactct gtcttgccga tagagtgttc
    13801 aaaggttctg cgtagctggt tccttgtcgc ctcaagaaag ttctcattgc gggcttcgtc
    13861 tggtttgaac aaaggtacta cccctttacg aagctgtcct ccaagagttc gtccaaacct
    13921 gttcattttt ccggcgtaac gtatacttgg cgacactcca agtattctta gacatttttc
    13981 tttgtttttt tgggcttcct catgtgtcct gctggtcata ggaatgagct ctgaattagg
    14041 ccataaatac atactgataa atggtagaaa aaaatatcag agttcggtgt gtcaaataca
    14101 gattattagc aatgtagagt cacgaaaggt attaaaagaa cgaacagagg aggacggacg
    14161 aaaaacattt caccttttat atatatgtcc cattaatgaa ccacaggtag tagagagggc
    14221 ggcgttatac caatatacat gaaagcagta attttagctg gtggattagg gacaagatta
    14281 caaccataca cattctttat tcctaagccg atgttgcctt taggtaataa gccattactt
    14341 gagcacataa ttgagtgggt tacaagtggt aatagtaata gcaatggtaa aattgaccat
    14401 attattctct gcgtgagcta tcttcatagg acgattgaag attattttga agatggtggt
    14461 agattcggta tcaagataga ctatgccagg tcggatagac ctcttgctac cgcaggacaa
    14521 ttaaaaacag cagagaaatt cttagatgag accttcgttt gtctctacgg agatgcggtt
    14581 tacgaatttg atcttgatga gatgatacat gaacataaga gttctaaagc atttatttca
    14641 atggcactac tctcgtataa aacaaatctg aggtatgggt tcatagatat taatgatgga
    14701 aaagaaggag caggtgaaaa acaacaagaa aaacaaaggc aaagaaaccg agtcatcaga
    14761 tggagagaaa aaccagaggt gagggggtta attaacatcg gttgttatgt tatggagccg
    14821 gaatttctca aacttgtgcc caagtcaagc gccttcggaa tggacgatgc agtaagaaaa
    14881 gctcttgaac aagatagact tgtaaaaggg tttaaaatag aatcaggatt tattgacata
    14941 ggagacaaga aatcatatct ggaggcatac aaaaaatatg tcaagaagct gggaaagata
    15001 taggcacata gagtggtatt aatacgaagc tattgtaaag agaaggcatc ttaacgaagc
    15061 tggattaaca ggtaattatt aaaaaagggt tcttatcaag agtatgaaat taagacgatg
    15121 tcccacctgt ggttgtagcg tggctactag ccactggtgc aaccataaca ataatgataa
    15181 aaaaatcgat cggacgtaag gctcacgcgc acttggccta tttgagttcg tagagtgcaa
    15241 tggataattt aaatacgatg ttttcattgg tcttttctgc tatgggtatt atgtactttt
    15301 gagtttcttc tttatatcgc aggatcttat tcttgtgaac ggcatttttt agagctctaa
    15361 cgttctcaag cctatctgct aggatcaaat atcgaataag tacagagctg tttgcgatat
    15421 tgtagaagta tttgtccagc gttttttctc tttcttcatt tcctattatt ggcattgcaa
    15481 caaggtgaac tatatcatgt atgacttcac cgaaatcatt cttcaattca ccatcatctt
    15541 taagtgtcgt atcgtgaaga agagccgcac atacgaggtc tgcattgtat atacgcagct
    15601 cttccgtgat aactagagcc accttcaatg gatgattgat atatggttcg attttgtttt
    15661 catctctgaa ctctccgaca tgagtcttaa gcgcaattcc aaatgctcta gttattttga
    15721 ttaaatcatc ggtcgaactc tcaaaaggtt ttattaatcg tacaaatccc cgtttagtaa
    15781 agtcggtgag atttgatttc atcatatccg tcccaagagc ttaatgtgta tatatcgtgc
    15841 tttgactaaa agtattgtgt gttttgccat aatttgttcg aatctcagat gccgttttaa
    15901 gagataaata gtatgcagaa gattgcataa cataattggt tctgaaggga gagactgctt
    15961 ctttctagta tcatgttaat ctagcggtgg tacctcagga gtgtatgtta ttagttacat
    16021 cagtccagca atgcgctact atcgttcgag ttctcgtcgc tttcctcaga tggagatacc
    16081 gtcgttgtta tattctcatc ctcgtcgcgc tcgtccacgg ttgaaccgac ggctgtgacc
    16141 tcatgacttt cgtaaattct cgtcgtaggc tctaacgatg attttgtgct ctctaatcca
    16201 gtcggtcgtt ccttacgtga gaccgttcta acactccggg tggatttctt tggctgtcgg
    16261 gaactttttt ttgtacgcgt gacactggtg cccctactgc ttggcctcct tgtactttgt
    16321 gctttagata ccttcctctt cggagtggct ctagacctct tagctgattt tgtctgcttc
    16381 cccttctttt ttcccgtggg tttcgtttta gttgccatat aattaccagt agataataca
    16441 aagataaata cttattaaat tttaactctt ctctacaata aattattatg acttgtccta
    16501 cacactaagt ggaatgccgt gtgtttgtat ggggtcttta tctataatat tatgaatttt
    16561 ataacctact acgttatatt ggtaaatttg tgccgggggc gcaaaacgaa accattatcc
    16621 atgaatcacg tctatgggga tatatttcgc gatggctcta cgcatagtgt tagaacagct
    16681 atccagtcca gaacggaata aaaaaagtac tacagcagtt caagttcaat ttactaaaga
    16741 agctttctct ggtcaagcat atatcatcgt tgactaatat ggtagaacgg cgtgtcgaac
    16801 gcttgctacc aagaataaga atatgcaagt aataataacc agaggagaga aaggttgtat
    16861 ttttggaaaa aatgacgata tcagatcaat gccgactata aatgtggcag gaaagggttt
    16921 caaagtgacg aacaccgttg gcgcaggaga tgcctttctt ggaacgtttt gtgcgatgaa
    16981 aattttaggc aaagacgatt ttgaatcatt attcctggca aacggctcta ttaaaacaac
    17041 gaaagaagaa actagaggaa gtcctaccct caacgaactt atcgagttta gtagatctat
    17101 gaaggcaaaa tacaaaaaat tagaatgagt atactgtgga ctgtatgttc gtaggattac
    17161 agcaaaatgt gctgataagc catagtacaa ctgcatccat ctcacatcag aaaactgatc
    17221 atggtcaagg gcacattgag gtcggacaaa gactttgtca gagaccatgc ttaattctta
    17281 cgagatacag aaatcaacat tccattctag actgtagcat aaaccataaa attatggata
    17341 aatttgttcg ccagtatatc tatctataat cttaattgct tttgtagggc aagtttgggc
    17401 cgcggcgagt attgattcga agtttgcccc agtctcagac tctactctta cctttggatt
    17461 tattctcttg tttttttcta aaacgaacac cttgggcgca agagtttcac aactgccaaa
    17521 tgccaagcaa agtgaatctt caatagttat ctggaatcga ccttttggag tatccaaaaa
    17581 gtttgaagct gttgattgca ctttagtata tttctttctg tagtcgtatt gtttatcgga
    17641 cgcatcgtaa ttcctactat tgctctcgta attagagtaa atccgattag tttccgaaac
    17701 attatgctgc acattagagc ggatctcctc ttcgtccaat tccgtttcga agtctgtctt
    17761 atcatacttg cgcttcttat catcgtcgga aagaatttcg taagcagcat tgattttttt
    17821 gatcatactc tcagcatgag aggaattgtt cctgtcagga tggtactttc ttgccaaacg
    17881 tctatacgcc ttcttcagct cgggatatgt ggcctgctca gaaacaccta gaattgcgta
    17941 atagccacta ctgtccaaca acctcaaaag cgtagcagga ttacttatct ctttagaaat
    18001 taagataccc tcaagtccgt attatctgaa tactgacttt gcattatctt aatattgaaa
    18061 ttattctgta agtggcttac tacatgtaaa ggagcttact gtttctgtgt attaaccaga
    18121 caaaaccagt ctcagaaggt tctgctgtct gcagtactag attatatttg aactcccatc
    18181 agataaatta atttgggctc gaacgaaggt caccacgatt attattattt ccttttcaga
    18241 cagggaaaaa ttttctactt cttacaattc aatactaccg gtcgaagtca tgatactaca
    18301 cctttaccat tatttgacgc atgttggaac cgcgaagata ctgatgttca tgtgacgcga
    18361 acaattagaa ggtaagacta atgagactag cacacctatg acacctatga ccatactcac
    18421 gatatgggtc tttgttaacc tttttactcc gcgattaata gacttcagaa tcttccttct
    18481 tcgatatctc tgattgttca tcaaaaatga atcgtccggt tgtctccatc aatggtccta
    18541 tgcaggaata caatatgtaa ctgtggaaaa tttcaactta tgtcgctggt catactaact
    18601 atgagaagta tcgtaaacta aatcgcgaac aatacgagta tttttaatta gtagcacttc
    18661 aaattctgca atgtatatag tggagctata aacgaacacc agcagaatac caatttccac
    18721 gaggtgcttc ttcaatcact actattacct cttttttctg taccttcagt attcttgtta
    18781 catcttcagt aattgcttct gccagacggt ccttttctat ttgtgttcta ccaccaaaca
    18841 ttgagactgt aactattggc atattgatga gataactaag aaaagcgatg aataagacct
    18901 tttcgtgtcc ctctgaacag gtttgaggtc gtcttgcata tacagcaaga ctccaaagtg
    18961 ggaattgagt agtgggatct actcaaggga tatcaaatgg ttgtaagatt attcttaagc
    19021 gtttgcctga aggtgatcac gatatctata ttgcgtggtc aacaccgcac ttaaacttgt
    19081 aaaatgcccc tttcgcaatt acgagcatac cctgtaaatc aaatagccac attctacgct
    19141 gtgtaattgc aaatcggcta tcatcacgga tacatccgag ccaggagtat ggagcgcctc
    19201 ggtttgtaac cctcttagac actctctttc ttcggattca gaagtatgaa acttgtccaa
    19261 atacatttca cctatcatga gttcattgac ttaatctttc ggaagaaaag cttttagtat
    19321 tacatctttt tatagcaaaa catgaaacgc gtagaagcca ttatcagtaa cgaaaaggtt
    19381 ttagaactaa atgaagcgtt gaaaaaggcc ggagtaggtg gttgcactat tttcgaatca
    19441 aggggccggg gcaaaggtgc aaagccggta gtggagagta gtagggggac agcaagatat
    19501 gtctctgaat tctcagttag agccaatgta cttacggttg ttgaagattc tgaagtcgaa
    19561 aaagtggtga acacgatact aaccacggtg agcacaggtt ccgctgggga tggcaagatc
    19621 tttatatcac ccgtcaatga cgcagttgac ataggaacaa aaaaacatgg agaagctgcc
    19681 gttaggtctg cgtgataact agctgattta ggttttaaag aatttctttg tttctccgag
    19741 cgctaacact ccaagtgctg cgatttctaa aacgataggt agatcggaga caaccttacc
    19801 tatggccagg cagcaacgat gaggcaatgt ctttactatt gatctgaccg tatcgttatc
    19861 agctaaagat acttttgata ttttgtccaa gtctacgtca ttcgtaaagt tgaacaaaaa
    19921 tatgttgtct gcttgtcggt aaattccgtc gccaatagca tctggctggt tcgtgataaa
    19981 agttggaaag attccaaaat gtctcatcct agttatgata tcctcccagt atgtttcccg
    20041 aatatacaaa tgggcttctt ctgcaaacag aaagatcgga ggaattattt ccttctctag
    20101 caagtcaacc aacttattta ggaccaactc taccaccatg cgtctcacca aggggattat
    20161 cctggccatg ctcaccacga ttaccgcgcc tttattttta tttgaaaaaa tattttcgaa
    20221 tttaagtcca tctgcacgat tttgtaaaaa taatcgagat gcctgcagta cgtggaacct
    20281 tgaaaccagc gcatcgcgta ctagctcgtt aatattccat gtattgatcg cgttgccgaa
    20341 agcttcgatc tctaggttat ccctgttttc aagccaatcc cagattcgga agaattctcg
    20401 cattgatgca gctggagtat cgagcgcatt cttgagtaca ttggagatag ccgccttgcc
    20461 acagtatgct agagagaaac ttaatccctt tcccgcttgc agtatcttga cctgatttgc
    20521 tagcgaggag ggagatccat catagttggt tgctaatccg ccgtactcat cattcaagtc
    20581 aaatacaatc acaaaggcgc cgtgctgcac gagtgtcttt ataagaatct ttgaaagatg
    20641 tgatttgcca gattcctttt taccggtaac gatatttagt ttgccgtcaa gatcctcagc
    20701 atatatttca aaatcttcgt tatctcctct ggttctgcct agtttaatag gaaagattcc
    20761 cgtctttccg agagactcgt tcaattcagt gatgcttaag cgccttagtt tggaatatac
    20821 ccttgatggg atccacgtca catcgcttga tagtttgcct gatgcatcta tagttccgcg
    20881 tattttagta cggaataacc tcgcatctcg gatcagtctt gataggtttg cgatgttgag
    20941 cgggtcatgt aaattttccg tactcgatgc agttattact tcatccctga taatatcttc
    21001 aatcaacgac tgggacgaaa gatactcctc atcataaatc tgagctatca tttttctgtt
    21061 caactttccg tcctcgatca agagatagtc acccttgctg acagcatcac ctttcattgc
    21121 cagtattaca agttcattgc tattttttgt taggactttc atgccggaat agaccctagc
    21181 agagttttac ggatatcttc tgatggcagc tcggtcacat catagttgct caagaggtgg
    21241 cctctaagac acgcgatctc tgtattacta aatgttgaga catggtgtgc aagccttaat
    21301 gtttcaggat agcctccacc cattgaatca tttgttagca atttaccaat tgagttgtct
    21361 atatcgccct cgggacaaac aatatctgca cgcaggatag gactattgtt atttccaagc
    21421 tttacaagga ggttatttcc tatagtgctt ctgcttaggc tctggataat catttcaatg
    21481 tccatatacg cagcctcatt gatatttgtg agaggtgccg agactttatc tagaagctta
    21541 aaggaggtgc ttttgctgat tcccaacaat acgttcttac gaacagaaca attctccgct
    21601 accgttgtaa tgctttggtt tttattctca aagggcgacg actttaatgc tccgtcaaca
    21661 acgattatag attttataaa atgatttgaa agctttatct gtatagcacg ttcaactcgt
    21721 agcctaatca gtcttctagc cgattctcca tcaaaaagaa ccaactttgc gagtctgtgg
    21781 tcaagttcgg aggtcttaat agtctccttg cttaaataaa ccaagatggg acctattttg
    21841 aagtgcatga ttgcctgacc gttaatagcg aatgcaatcc cactttttat ggcgtacaga
    21901 gaaccgtctt cagtttcggc tatcctgatg ctgctggaat cgactgcggc tactagtgca
    21961 gcctcattaa cgggccggat cggctttatt gttttttcac aacctggatt acatctgatc
    22021 ccccatcctc cgagtggtac atgatttttc tcgtaacagc taaagacaat ctttttcccg
    22081 ttaagaacat tctgcaactt caatgacagg cttcctccta ttgactcgtc caaactacgt
    22141 tgaaattcat taaagtcaat ctcggccgaa ttcaattgaa tattcatgtt gtgcaacaat
    22201 ataacatgga tagttattaa tgttgtgcaa catgcacaag atgggcttgc tcagcaacaa
    22261 gtttatgtga tagaaatttc accgttttat atcactgtta atgaatgaat caccaccttc
    22321 atcggatgaa gttagaatta gattaaaaca ccgcgactgg gaggtggaaa tcagctgtat
    22381 cgagaaaaaa gtgaaagagg tggttgaaaa tgtgctgtca gggatcgata gttcagttag
    22441 cattaactcg gaattcagta ctcaaattgc agaattaaga aggcagattg atactctaac
    22501 tgagcaggtc cattcaaatg ttatcattca aagacagatg acaaatccag tcactctgca
    22561 aaacaagatg gcgcaaaggg gggggataac atgtagaggc ctcctccaat cgctgtggga
    22621 cgaaggatat tttggggctg aaaaaccact aggggaggtt catgaggagc tgtcaaggcg
    22681 gggttttaat tacgatagga ctgcggtttc tcattcactt acggacatgg taagggaaaa
    22741 tattcttacc agaaaaggaa caatgagaaa ttatcaatac atccagagaa agccgccttc
    22801 tgaattgccg tcacaataag attcgtctat cattttaccc ttttagcgag cattgagttc
    22861 aaccagtgaa taccagaagt agtaatttta taggcggaag attcatttcc atctgttgtg
    22921 ctgtccctta gcacgtgtcc tgccttaacc aattccgaga gccttgagct gacagacttt
    22981 gggttcaacg tcgttacgaa ttgaatttca gacaattgcc ttgcatcatt agacaccttg
    23041 ccaagatcct ttgcaatttt tgctgctacc aagtgcagcg caacattctc tttgtcagac
    23101 aattttttca tcccaaattc ttcgtgctct aggattactc gcggaccttc tggggttatt
    23161 ttaataaagt tcgaaaatag atcaataagc tcggtaactg cataacttaa cgaaattttc
    23221 ctagccaaat cgatcgcggg aacttgtttt gtcatgaagt taatgacaga tgtcaagacc
    23281 aaatcaggag aaccgttaaa ctgtaccttt acctctccga cagttatcga gacattcacg
    23341 ttagtactac cctcagtgtt aggagtatta ttaagcatct tttatgctcc gctcaaatga
    23401 tattgctttg actcatgatc atttatcatg tcgggtatct gtctaatcac agaagggaat
    23461 acattttgtt gatttgagaa aaaaggtcga aaactgttat ggctaacact tactttctgt
    23521 gtacggccga tagtggatgt ccatcttaat atcctaaaat gttgaacgca gctcatattt
    23581 gataaaccga tcgtctgtat gattttctat attatataat aagatactgt tgtctcaaca
    23641 ccattattct actatatctt tcttgatccc tagagaaaag acaataaatc atttacggaa
    23701 aaacataatc ttataagtaa catggcaatg ttttatataa actccattct ctaagtcctt
    23761 tcataaggca tatttcatcg cccagggaaa gaccgatcac gaacagtact ggaggggcca
    23821 tacgaattag ctactgctaa attcatggta gaaggatcag gagccacaaa tgatcatatc
    23881 ctccctattg ataagtaaaa gaggaacggc agcatatgtg aggatagtca gcgatacagc
    23941 tacagcagaa ggacagcggg tttattatag agaattatta ggcttttatt gcagaagtag
    24001 cactgtcatc ctatcaatgg ttcttgacga taatacccta ctccaggccg atgcaacagt
    24061 gatagcagga gttctaatac ttctgactat ttactcctta agacaaaaac cttcagaaaa
    24121 tacccaagat aggatcatga acattgtcat agcaggagtg ctcgttactg ttatagtgcc
    24181 gttttctggt tctgcaatgt tgattctttc tccaaatcaa gaatcagctg cagtaatagc
    24241 attttatggg ttcatatatc tatttgtggg gatcgtatta atggtgttga tatctaggat
    24301 tcccttattt caaaagaaag tgactgggaa tccctagcta acttattttt acctcaaaga
    24361 accttttctt taaaacacgc tgattcatta tatgttataa catataacat ataatgtgat
    24421 gtcgttgatt tcaagaaata agacaggtat ataaaattaa tacgaaaaat atagtaaaac
    24481 taacgaagat cgggtaggag agaacctaaa tcttcctctt tgctcaatac tagctcatta
    24541 aacgacttta ttggcctgta aacaccaggc gttaccaaca tatgaaattt cacaaccata
    24601 aagtgtaaca aacacaaatg gaaagcctac ggattcggca ggcgaactga ccgagttgag
    24661 gaaataaaga catttttcga atgtctacag tgcaataagc taagggaggt cactaaatga
    24721 ccagcgaaac cttagactcg gcgttaggta tggaacgact agaggacttg gaggaacatc
    24781 ttgaatcaat attgcaaaga aggacgccga ttgtttcaac tgaattgata gctatgctgg
    24841 cttcacggaa gaattttgac cctgagaaag aacgcaggtt aatcgaaaaa tcaaagacga
    24901 caaaatgccg ttgttgtcac tacagcggga ctaggccatg gttttaccga acatgcaggc
    24961 accttccaga taatcactat gtctacgaag tcataaggag cctaatcaga tgatgctacc
    25021 tcccgaacaa gaagcaaaag aactgatgta gatatacaaa gatattgcca cggtgatacg
    25081 ttacagttct tctttatctg gtagagaaat caaggatcat agagtatgat agaatcatca
    25141 aagataatga tttgtggcct tattacgatg tcagagaaag tatgttgaat aagaagcgga
    25201 gagagcaata ggatgtgata aatattgaac atagaagaaa tccagaagaa attaatgttc
    25261 atcgtaatat ttttggcaaa gatccaattg atgtagatta tgatagtatc ggtccatgtc
    25321 ctttctgtaa ttctcgaatc gacgagttca atttttgggc ctgcggcgga aaccttggta
    25381 tcagttagaa gtaacctttt tctttagccc tcacctcttt gctatcacat tcagtagtgt
    25441 tcctgcttca acaaaattca tttgggtctt cacctcttat tctatcgact ccgtcagatt
    25501 tagaaaattc cagtattact cctgtctcag aatcatattc aaatttgtat atttcatttc
    25561 agagataggg attgatacta atcttgcgtc actgcaggaa ggacatttag taaccgtcct
    25621 tgttgctaca ttaagacaag atgcgcacca aaaacatgat ttacataata gaaaacgcat
    25681 cttccttgca actattatgt ccctgttgtc tatgtcagtg atatgctcct caagcctata
    25741 ataaaagtct ctatacttag tatcttccag attgaggtta ctgatcaatt ttctctcata
    25801 tagcga
//
LOCUS       DAOR01000130            8847 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_68223, whole genome shotgun sequence.
ACCESSION   DAOR01000130 DAOR01000000
VERSION     DAOR01000130.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_68223"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2548 a         1948 c         1779 g         2572 t
ORIGIN      
        1 tctttagcac atataatgga ctacctgcta gaacaccctc tagatctggc gctgttattt
       61 ttacgcctgc tgctgcaatg acttcagtga ctggtctaaa tttatctctc ggatctcgca
      121 tctcatctag cggttttggt aggagcaagg acttgatttt agtaactatg gcagagtttc
      181 ttttcgccaa cacaatatta tcgccttgtc ttaatactcc atcaagaaca atagtatttg
      241 cagacggacc taagccaacc tcgtcggtga tttcaagaat tattcctctt gctactccat
      301 cgtgtctttc caacttggta gccatatatt gctgtgcaag accgaccaat accgcaagta
      361 attccggtat tccaacgccc gttactgcac ttacaggcac gattgctagt tcctttgcga
      421 aatctttaac tcgccagaat acctctgaat tgtaacccat tcttgaaagt gcacctacta
      481 ccccgtatat tttttcatct aatagggtgc tcacggaact atcctgttct ttaatttcat
      541 ccgtaatgaa ggagaccttg ttttttgatt gatttgattt acgccagcca ctaacgacgt
      601 caaccttatt tagagcaatc acaaacggca cttttctttt tgcaagtatt cccacacttt
      661 cgatggtctg aggctcaaat cccttgttta cgtcaacaac aactatcgca agatctgctg
      721 ctgaacctcc acgaattcgt agattagcaa aaacctcgtg tcctggcgtg tctatgacta
      781 gtaaacctgg aatctgagtt tcagcttttg ccagtttttg atacaacagt cctgtaatct
      841 cttttaccgt ttcgatagga aagaagcttg ccccaatgtg ctgtgtaata cctccgacct
      901 ctctggcctg gacggccgtc ccgcgtattc tatctagtaa agaagttttg ccagagtcta
      961 cgtgacccaa aaccactact acgggctgtc ggagctgcac ttatgtaata cgatcgaaaa
     1021 tgactcggga ctcttttatg aagctttcat atgaatcaga ggcgtgaatt acattgtctg
     1081 ttagaccaat tccaaaatca cctctaatgg ttccaggcgc cgcctcaaaa gatttggttg
     1141 ctcctatcat taatcttaca gaatttaatg cattagttcc ttctactata cacgcaatga
     1201 ccaaaccgga tgagatgaat tttactaaat cgttaaaaaa tgctttatcc atatgcggtg
     1261 cgtagaattc ttgagccttc tctatagtaa aattaaatga cgtcatattt tttatctgaa
     1321 aacctttttc ctcaaacctc gccagaattc tgccagttag ctgtcgtcta acgccgtcag
     1381 gtttaacaac gataagagtc tgttccacta ccatggtgtt acttttttac cttaatacca
     1441 cctttcacgt attttgtggt ccatttgaac tttcgggggt cacgcttcag cttccggaga
     1501 ttctttttgc atttattaga acatgtccac tgaattgagc cgtcattttt gacaagcatt
     1561 gtcccttgcc ctgtagtcac atgcctgcca cagaaaaagc aattacgtac agatgtcgcc
     1621 gtcttgctca tatcctatac ctacatgata atgctcgaga tatcacttta tcttccgcgc
     1681 ttctctttca gtttccctga gcatcaagat atcgttcatt ctgaccgaac ctttgacatt
     1741 tctagttaaa atacggccct tgtcttttcc ttccaggact ctaaccctta cctgtataac
     1801 ctcacctgca atacccgttc tgccaacaat ttggatcacc tcagcaggaa taaccctttc
     1861 ttccgatgtc ttactcattt aatgactatc acgttttagc cttaatagac gtaatcgagc
     1921 ttacaacttg ttcaaggata tgctgacctt ctcctgcatc taaaatcgtt gcagccgcag
     1981 agcccacgtc tattccaaga gacgcaccaa gctgctgctt gctaggtaca aaaatgtatg
     2041 ccgcattcct ctcttcacaa agaattggca aatgggcaac aacctcagga ggttcaacgt
     2101 cttctgcgat gaccactagt ctactggtac cccgctcgat tgctttagtg gtctcattgg
     2161 tgccttttct taccctcccg ctctgctttg ctatacgtac agcctcatat acagcattaa
     2221 ctagatcttt tggagtttca aactttacat aatatggttt actcattgct taattatcac
     2281 ccattgggat catcttggtc ttctctaaga ggcaaataac agtattaaaa tgttgtctat
     2341 tttagattca ttaagcgcta ttaatagtac cttcaatcaa tcgcctatac tctctcactt
     2401 ttgaattcaa cagcatctcg tctgtagatt ctgcatacat tctaactaat ggctctgtgc
     2461 cactcggtct taccatcacc caagtttctt tatctatcca tatctttgca ccgtctaaag
     2521 tttcaacgtt ctgaaagctg ccgtgtttca tacatgattg cacaacctca ttcgctttct
     2581 gtttgtcgca tctaaatttc gctttgtatt gaaatatttt tggtaacgat gacatcagag
     2641 ttgaaagcgt ttggctctct gttgccatca tgtctaatac aagtgctgtc gtcatgaccc
     2701 cgtctctgac ttcatttaat ttcccataca tgaaaccacc attttcttct agtcctatta
     2761 tcgattttct cttgaccatc tctcgtgata cttcaacgct cccaaccttc gtatgaataa
     2821 cttctgagcc ggcttcattt gctactatag aaacagccat cgtagaattt atcggacata
     2881 ccacatccgc acctctgtgc ttcgtagtta acaaatgatt cacgattttg gctccggttc
     2941 tatcgccggg gtgtactact cctttttcat cacaaaacat gctacggtct ccatcgccat
     3001 catacgctac tcctagatct gccttcgtgc ctctcacaat gctagataaa acttggagat
     3061 tatccagcgt gggttctgat tctcgtgcgg gaaatgtgcc gtctatagta ctattcaaag
     3121 caactatctt acaccctaat ctttcggcaa gtaagggggc gacagtcgcc tgtacaccat
     3181 tgccggcatc cagcgcaata gtgaaccttc gttttcgaat cgtcgggata tctatcaact
     3241 ttgtaacttc gtttaggtaa gaattgatta ccagggggtc aggaaaatcc agcccatatc
     3301 catcgatttt ggaaaatctc tttgagtaat aaatcgcttc aacatctaat tcgtcttctc
     3361 gtgaaatctc tacaccgtcc gctgcgattg gcttaatccc attatattca gcaggattat
     3421 gagatgctgt tatcattatt ccaccgttgt agtcaagaaa ctttacggca tattgcagac
     3481 aaggagtggg cagtaaacct gcattacgca catcgacgcc tgcggagttg attgtcgaac
     3541 ggacaatacg tgagataatg ggactagact cccttccatc gcaccccaca ataattgggc
     3601 cgttttcgta gtaggtcgct agggagtaag aaagatcgac gattaaatcc agtgtaagtt
     3661 cactaccaaa aactcctctg acgccgttag ttccaaataa cctgccatta ctgtctggca
     3721 ttatttcctt caccttgata ccggagtgaa taatttatta ctgttgtgat tctattcgtt
     3781 gaatatcgaa tcctggcata gatttagcgc tagcatctga aaatccttcc acatattcca
     3841 gcaatgtctg ttttccaaga caccgagact gttcttccaa actccgtgta aatttttggt
     3901 tagattaagt tgaaccgacg atgaataaga caaattccta tatcgtggtt caattcaccg
     3961 gaaaggctca gttgatgaca tcatgactga agcacataag gagttgctaa cttattttga
     4021 tcatttccaa gataggatca ccttagcaca tccatgatag taatatatat gagatttttg
     4081 agaaatgctt atgttcaaca accagcctgg tcagagcaac tcacatctta gaatcatggt
     4141 tgttgacgat gagaaggata tattacgtat aataaaaaga gatttagaat caaatgactt
     4201 gaaagcaagt ttcaaagttg aggctttctc cgacagcgag tcagcactga acgctttcaa
     4261 cagccaccct gctgactatt atgatcttgt cctcactgat attagaatgc caaaaatgaa
     4321 tggttttgaa ttatatcgac atataaaaca gagaaaccca gcaatgaaaa tcgcattcat
     4381 tacggccttt gaaattacca aagaagagtt tagtaaggtt ttaccttcca tagacgttga
     4441 gcatttcata aagaagccta taacaatgtc aaacctgata cctaggctaa gatcgattat
     4501 tgctaattaa gaatctagat ttgcggccca ctcactatgt gtaaatgaag gctgaacggc
     4561 atgagccccg caacatcata gtatagtaga ataatagata aattgctcaa tgtgggggtt
     4621 gagtatgcgg gggttgccaa gcctggccaa cggcgcaggt cttctctcga ttagagatgt
     4681 gcgaggctta gaatgagcca ggtggatgaa ttagtgactg gttcctagaa ccctgtttct
     4741 tagggattcg tgggttcgaa tcccaccccc cgcacccata tagctgctta caagagtacc
     4801 gcatggccaa gggacttgac cttgcagtga cgataagcgc aacggaatga gagatataag
     4861 ctgcagaatg aaagcaatct gcgaatccga aattaggaag gcagcttctg tctattccca
     4921 accattcttt caatcatact aggtgtaccc atgttccgga gagatttgtc aacccctgca
     4981 ggaactcaga taatttgaat agccaatatc tagcgatgtg catatctggg taagattcta
     5041 cagcacggtg tggatgcagt tattgttttc tttatgagcc tgaaaatgac tctggcagaa
     5101 atcattattg cagtccccac aatgccaacc agacagtgta taacaaacta cgcactttcc
     5161 tattggcttg ttatagtcat attgcttatt gcctttgtcg tgttgcttca tggagcaaat
     5221 gtcctgtcct actctttaga gccaatgatt ccatctgtag atgttttatt ccctaaaaag
     5281 tcaagtcggt cgttacaaga tgttttcgtc aaatatcttg cattttcttt catagctaga
     5341 aatttaaagg tttgtttacg gtgaacgttt atcaccaaga tttgagattg aacatctagc
     5401 aatggttgta ccgaagacct tcatcgaagt tactgtagtc gggaatgatc gcaaaggagt
     5461 tgttgccgac gtcacgaact tcatattcac aattggtgga aatatcgaga aggttaatca
     5521 gaatgttgta aggggattgt ttggaatgca actcgaggcc tcttttcaaa cgatcgacaa
     5581 aaaaaaaatc gaccttggaa tgcgttcgct agcaaaaaaa ttggggatgg aaattagagt
     5641 ccactatgaa gagccgagtc ggctcagaaa catagcgatt tttgctagca aagaaccaaa
     5701 ctgtctccta aggattttag aagccaagga gaacggctta atcaacgcca atataccggt
     5761 tattataggc agcgaaggga ccatgggacc aatagctgac ggaaatagga taccgtttca
     5821 catcgtcgat gacaaacaag attccattct tgctgaaaac aaaatattga aactaacgga
     5881 acagtacaac attgatctca ttattctcgc acgctatatg cgtatattaa gccctaattt
     5941 cgtttggcgg tatcctaaca ggattattaa tatccatcca tcgctcttgc ctgcgtttcc
     6001 tggagcctat gcctacgtac aggcatatga gcgcggagcg aagatagtgg ggtgtacggc
     6061 gcattttgtt acggaggacc ttgaccaagg gcccattata tatcaggagt cttttagagt
     6121 taaagaactg gatacgcttg acactatacg acagaaagga caatccctag aagcagaaac
     6181 tctccttaaa gccactaaat tataccttga aggaaaactg gagatttact gggggagggt
     6241 tcatattcta ggaaacatct gaacgccatt ttatttttca aagtacgaag cagccccttt
     6301 aactatcaaa ctggattcta gacctatggt gatgtaaagt tgataaatga gcaggatctg
     6361 gtgaagtcct aaaagatgtc gatacctatc tataacatca gccattatga gtttcgtaaa
     6421 tgtctcaaaa gaacccgccg agcaatagta cccgtaggtt cacttgagca acacggtgct
     6481 cacctacctg tgtctactga ctgtttaatc tcagaacggg tcgcgacccg agtggccgat
     6541 gcagttaaat gtttcgtttt gccggtaatc tcgtacggag tatctttcga acatattcca
     6601 atgtttaatg tatctttgag aaattccacg ttatctgacg tgctgtgcga ggtttgtgtt
     6661 tcactttcaa gccaagggtt aacagagatc atatttatca atggtcacca tggaaatatc
     6721 ggagtcctac aatacatctc acagaatcta gacgcaaaga tccgcactaa gagcagtata
     6781 tatacaatta attactggag tgggatgcaa aaagaacttg atcacgcagg cgaagtcgaa
     6841 acgtcccttg tgttggccat ttcgccccag ttggtccgaa tggacaaagc agaaccaaat
     6901 ctcaagaggc tgtctaaatc aagaattgca tactcaacta taactaccac acctggttct
     6961 tttccccagg tgaccggtaa tggggtatgg ggagagcctc gtaccgcaag taaagaaaaa
     7021 ggtgatagac tcctaaatga aatagtcggg aatattgtgc atatcatttc agagttacct
     7081 caggaatgaa attttaatat atacctccct tatgccatac ctataggtag acattattct
     7141 tgtcggtaga catggcagtg aaagttttgg atgagagtct tggaaaagtt gtgcttataa
     7201 aactgaaggg cggaaaagtt atcagaggaa atcttcacgg attcgatcag catatgaatc
     7261 tgttgctgga agaatctgta gaaatattgg atcagggtaa atctaacgac ttggggacta
     7321 ttgtcgttag aggtgacaat gtggtcataa tatcgcctcc accagtgtaa aacttcctgg
     7381 gtgagttata atgaccaaag gcaccacttc gatggggaac ttcacgagga aaaaaaccca
     7441 catccgatgc aggagatgcg gccataattc ttttcacaaa cgtgggaagg aatgtgccaa
     7501 atgtggtttc ccggatgcca gaatgagaaa atacaactgg attaagcgtc gagtgatatg
     7561 ataaaatagt tatttctttg tctatttccc ggtcagaagg ttgccaaaat tgtctgcctg
     7621 cagaatcaga aaaatttaac tatatcacgt caggaataaa tctcgacatt tgattgaaga
     7681 aatacttgtc ctcgtgtgct tttcgttggc tggggcgata tcggctctta ctttttggaa
     7741 acactccgag agtaagttgg ctctcctaaa acatagacgt aatcaggttg taaaaccaat
     7801 taatgccaca agatcagaaa tcgaagcctt gcaattcgag aaaagcatac tatcacactc
     7861 gattaccaac atttatgaag ctgttcaaaa aggtaagata ggtgcgatcg aacgggaccg
     7921 gctcttgtta aaatacaaat cccagctaga cacctttaat aaaaaaatag gagaattaca
     7981 attggcggtt gactttacag agctatctga catgcgcact caacttgttt ccctactgga
     8041 agaaaggatc gaaaccatag aaggaaggct caccgaaata tccaagaagt gcgggatgcc
     8101 actggattcc ctcttccatg taacaaagcg aaaatacgct acagtagaag ctaggggtca
     8161 ggaatattcc gaaataggtg agaagaaaag acaagataag agagactcgg cttcggttag
     8221 agtggaggat aaaaatatcc aagaggttca acaggaagtt atgcaggccc taactcgcct
     8281 agaacacgct ggagaagttg atgacgatat cgtgacacaa gttgaccaaa taaagcccga
     8341 tggttttaca gagaatgttg ccattccctc ttcggacttg gatgtagatc gggaaagcca
     8401 tccccacaac aagacccgtg atgctctttc gtttctcaat gaagcagaag tagaagacta
     8461 gttactatga agcgccgaca taaagttctt ctccatgtgg attattcagt cataatcggt
     8521 acctaaaacg aatattaacg tggccatctc tctgctctag aagttgtcat ctgcgtcctc
     8581 gctcataaag agtgcactta ttaacagtgc aaaagatgcg gtgaagcgat ttgtaacagc
     8641 gaaccaggtc gtcggtttag gcagcggaag tactatgaca actcttgtga gggaattggc
     8701 gaatttacca tacaagaggt cagttgagtt cgtgacaaca tctcttcaaa taaaaatcga
     8761 agctgaaaaa gtggggctca aaattgcaga cgaaggtagc tttgtagaca tcgatgtggt
     8821 cttcgatgga gcggatcaga tcgattc
//
LOCUS       DAOR01000131           15340 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_24082, whole genome shotgun sequence.
ACCESSION   DAOR01000131 DAOR01000000
VERSION     DAOR01000131.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15340)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_24082"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         4567 a         3043 c         3302 g         4428 t
ORIGIN      
        1 ccttgaatgt tatcgcaata actgggacac cggtcttttc tctaatttct tccccgtgta
       61 ttatgttgta catgctaata ataaggccgt gaagcattat gcaattaacg tcgttccttt
      121 ttaatgacct aatcatggtc aagatgtttt gtgtagaatc attgcctcta atcgtgacgg
      181 taccaaaaac cataccatca attatcagat cccggcgcat tactaccccg gcaagtgtgg
      241 aagtcgcgca ggatttcttg aagctttccg ctattccaaa tactcggatg ccttttttct
      301 ccgcgtgaaa cctcgtccag cggcttcctg cgtcactgtg tttgaagggc agaatagaat
      361 tggacacaag tctgtattac tgagtggctg ttaacagact ttcctattta actgcgaccc
      421 aggcctcggt tacgatttgt gatgtactcc aaggttacca tgactactcc tcgtttgtct
      481 aaacccaaac cttaactcag acgtcataat ccacataggc atagccgtca gggatgaaag
      541 atttttgatg agtaactctt acagtggagt gtgagttcct ctattcttta cttactccat
      601 gtttgctacg gtgggcagat tgtgaataga atcaccattt tggagttgtt cttcatccat
      661 cgtgaaaacg aaacgcgatc cttcaaattc tgctgaacca actctctccc aatttatttt
      721 atattcatga gttagttgtt taccaataat tttgatattt gtcggcagac tggaatagag
      781 tgtatccact ttttggggat ccctggttcc taataaggat ctacgtcttc tcattaaata
      841 tttaactgct acgttcttat gagcatgccc ctccacagga attttttctc tccccgtgtc
      901 tatggtgaat tgacgttttg ccaaacggtg tgattgaccg gcattaatac aaaagttttg
      961 tggtcataac atggatacaa aatagatagc aaacaacgcg catttaccac gcggcagggg
     1021 gagagcatca acatgacaag tattgaagga taaatggtta tttggatccc aaaggtggga
     1081 gacgatgctg actgcgagat tggtactcag aaccagggga ggacgccaaa attacgaatg
     1141 ttcaaaagta atttcgaaaa cctcacaagg tattttattc atccaaattc aaagcaattg
     1201 agtaaattta ctaattgctc ggactgactc caatctctaa agtatgcttg aatggtgtaa
     1261 cgccctgaga attagtgggg cttgctgcgt agccagtaac aattttggat gaggcgtcgt
     1321 tgttaacgct attagcaaga ccgggattga tccaaccaca atcatcgtca ggactatcag
     1381 ccatgttgcc gtgtgcatat tcgtacttcg ggacaactgt atataaattt tcaaatttct
     1441 tggattacga cgcgtcacgc gcgggttatt tacgatgatt ccatttgtag cgttcaaata
     1501 agttacctag gtatctcatt gatcaccacc cacgtatgaa agaagaatgt gaagtgaaag
     1561 tatgcagatc tggttgagat atctgcgttc aggcatatca aatttggatt actaactgtt
     1621 tgagtggagc aatatgtctt agcttacacc tactctaaaa aattggttta taccaatcgc
     1681 cacaccctcg caacttaaag cgccgaagaa aataggatta taaataagcg catttgaatg
     1741 tcctctgcgc ttacatgaga ttgcttgaat ttcaaggaaa ggaacttttg tcgcagtacg
     1801 gtatctcagt cccggcagca tctctcgtta actataggga tgaagccatt caggccgcaa
     1861 ccaaactcgg atatccggtg gtattaaagt cgcaacttac ggtcggcgga aggggcaagg
     1921 caggtgcaat cttaaaatgc aaaagcgcct ccgaactttt acctaaattc gacgacctca
     1981 taaacaagga ggtaaaaggc gaaatgcctc gggggatttt ggttgaagag gtggtagaca
     2041 taaaaagcga actttattta tctttttttc tgaacagaag caggagatgc tattcattaa
     2101 ttgcgtcagc agaaggagga atagaaattg aaagcgtgga caacaaggta atatttgata
     2161 tccccttaga gggattatct cctgagattg ccgagggcgc agcccagaaa ttaggtttaa
     2221 tgggcatcac accaaccgcc tttgtagatt ttacaatgaa gctctcaaaa ttggttatgg
     2281 aagaagaggc tgagctagcg gaggtcaatc ctatggccat gttgagtgac ggctctctga
     2341 tggcactaga cgcgaaagtg ataatagatg acaacgcgat gtttagacat gccgaactaa
     2401 agaagtacga gcatatttcc gaattggagg aacaagccgg aaagagcggc ttttcattgg
     2461 tggaactgga tgggaatatc gccatcattg gaaacggggc cggcttggta atgtctacag
     2521 ttgacatggt cacagatgca ggtggtaagg ctggctgctt tcttgacttt ggaggtggag
     2581 caactactga gacgatttat gaagcgctaa cggtcattag caaaataaga agggttcgag
     2641 gtatcctggt gaatcttttc ggcggaattg taaaaacaaa cttagttgct caagcaatac
     2701 tggatgccta taaaaataat ctgatcgacg tacccttgtt cgcaaggatc accggggcag
     2761 aatctgaaaa agccaaagca atgctcgctg gaagccaagc gaaattatat gacacggtag
     2821 aagaagccat acatgaggta gttgaccaag tttcgtgata gagaggagag aaaatagtgg
     2881 ataaagaata cgacatattt gagatattgg tcggcaggga agaagatgag gattacaaga
     2941 aaaaacctgt cataattcaa ggaataactg ggagctttgg ttccacccat tctagattaa
     3001 tgaccgccta tggcacaaat attgtggctg gtgtcactcc tggcaaagga ggccagacct
     3061 ttgaaggaat tcctgtattt gacaatatgg ctgaagctgt taaatcgaca ggggcaaaaa
     3121 tttctgggat gtttgtacct gcatcttttt tccttaacgc cgcagaagat gccctagata
     3181 gtgggataaa attattggta gctgtcccgg agcatgtacc aataagagat tcaataaaag
     3241 ttttagagta cgcgaagaca aagggagcaa aaatggtggg acctaatacg cctggcgtaa
     3301 tcgtccccca agttatgaag gttggtatca tgccggcaca accctttaag gcaggtgaca
     3361 cggtagtgtt ttcacgcagc ggcacgttaa tgtatgaggt ctcttataac ttgaccaagt
     3421 cgggattcgg acagaggtta tgtcttggaa taggtggcga tcccataaac ggaacaaatc
     3481 taatcgaagc ttttaacatg attaggaata aagatgatgt tcgatcggtg gtagtcgtag
     3541 gcgaaatcgg cggtgatgct gaagaactac ttgcagatta tttgatcgaa acaagattcc
     3601 ccaaagctgt tatcgcgtat atcgctggga gagctgcacc aaaagaaaag agaatgggac
     3661 acgctggtgc gatcgtctat ggaaactatg gctcagcaga atcaaaattt atgaaatttg
     3721 aaaaggcagg tgtgccagtt gcgaaacgac cagctgaagt gccgaaatta ctcgcacaga
     3781 aactacattt gcaagtagag tgaggcaaac aaaccataca ccggcatata aggaaattct
     3841 ctacgacaca aattctccat ccactagtgg ctgctctcgc ctttttcgat gtaccgagat
     3901 atgcgaagaa actaatgcta agaccagccc gattagattc ataaatgcaa tcaggtggga
     3961 atgtagctag cttttgacaa ctccggaaaa gcgtttctgc gatagatgta tgataaggac
     4021 gatacatgat gttattgagg aagaggaggt agtttatacg tacaagcgta agaggctcta
     4081 ccgttgtaaa acgtgtggac gtacgagttt caaaagagga ttgagaccta gtgctgaatc
     4141 cgtttattga ataggtgaag tttctgaaat gagctgttca gtcacacacg gtattgtaaa
     4201 attgaaaaat gaatttatca aacatcacgc tacccgaagc attgtcagag aggtaatacc
     4261 gttgtcgtat tctacactaa taccgatggc tgaacaagcg ttattgcgta taaacgattt
     4321 tgtgaactgc aaccccatct atgttgaatc cgaggatata catgtatttg atataccgtg
     4381 tagagcttac ctcggtgata tcaacgatta ctggttggat acgaaaaaat atgatgcaaa
     4441 ttaccagccc ttttacccca cgtggatgct atctgcctac gctttagcat tagaagccaa
     4501 attattaggc ttcaaagata ttgttgatat agggtctggg gacggtagaa ttgcatactg
     4561 tggcaagtta ttagggctta ggtcggtcgg gatagaaatt gatagcaaac ttacaagcct
     4621 ccagcatgaa atttctgctt taactgacgt tgaatacgag atacatcaag gcgatgccac
     4681 gaaatttgca tacgattcgc ttcatctttc aagacctatg ttctttattt cgggattgcc
     4741 ggaatggggt gacatgcttg caagctttgc gattccccgt atcatggaaa tatctgagct
     4801 aagagaatgt gccggattca attttatggg aagccacata atgaaaaaat attcgagaga
     4861 taagaccgga tggggatggg gtaaaattat cgaaatgtat gacctcaaga taaaaaaatg
     4921 tattacttta cctacttgtt ggactaacga ccagacgttt gatactgctt acgtattcac
     4981 aaccactcaa taaatggtta gggtgcagca tacttttttt cttgcgatat ctgttatagg
     5041 tgtgtaggtg agtgcttggt ctaaaggact tttgtgcatc atgcaaaggt acattattca
     5101 tttggaaaat gtgaaatata tctcaagaga ggccaattgg ctactcaaga cagctcggag
     5161 cctggtatcc gatattggag tcattgtacg agatactcgt gttgcaagca gacatgtcga
     5221 gtttgacaca agcgttcctg aaaacatcag tatggaagag gttctccgaa gatttgccac
     5281 aatttcgcca atatctgaat atgaacattt agtggaaaaa cgaatgggaa agcatgaagc
     5341 aatcctcaaa gcaaaggatt tattcaatga tgaaaagtat tggggggctc acgaagctct
     5401 tgaatctgtc tggaaaaaca ctcatcatga ggaaagggac ctgctgaatg gaatcatttt
     5461 gattgctgct gcattcgttc atgaccaaaa agatgaatat agcatttgca ttgccatact
     5521 gagcagagcg atggagaagc tatcacaagc taagggatta tacttcgaaa tggacttgga
     5581 cgaaataaaa aaaagggtgt tacgaataat tgaaacagga aagatagaga ggtttacaat
     5641 ttagagcgtt tttgcatctc gatacatagc ttattgatta caggcgctaa attgcgctca
     5701 ccattactcg attaacaatt gccgggacgg tacctgtcta ggcagaacat taatctattt
     5761 gattggtgga atacttataa ttcgtacaaa ggcggaaaaa ctcgttaccc acacaacctc
     5821 cctggacctt cattttgtgt ggacaacgct caaaaaattt aaactggtat cttccgtagt
     5881 atacaagaat tcgaagtgat caatggattc cctcgtatac tagaagagat gaacgcaatc
     5941 caattaagaa aaaatctaaa tagtaagaca gtagctatgc tcgtttttgg tgcatgtgag
     6001 aatcatggtg accacatgcc ttttggctct gacttttttg ttcctatgga attggcgaaa
     6061 cgtgttgcgg cccgagcagg gaatgtgttg gtgctacctg cttttcccta cggagttagc
     6121 tcacatcaca gcgactttca gatgacgata acgttggagc caagtacaat gattatggcg
     6181 attgaagaca tatgttcaag tcttatcaaa aacggcatta acagaatact cataataaat
     6241 ggacacgacg gcaatatcgc acctatagaa tcgtctgcaa gaactattaa agatagatat
     6301 cctaacgtag tgatagcgtg ccttgaggca tggtggatcc ttgtggggaa gaccaaaaat
     6361 ctatttggcg aatgggctgg attcggtcac ggcggagagg ctgagacttc ggttatgtta
     6421 tcagtccggc ccgatctggt agacatgaag tacgcgccca agaaaacgat tcctaagcta
     6481 ccacggaacg taaggatata ctggagattc agcgagctaa ctaagactgg ggctacaggt
     6541 gccccgcggc atgcgagccg gtcaaagggt gacaaaactc tacatatact tgaacgcctg
     6601 ttacttggat ttatcagtga tatggaaaaa aataattgga aatacgggct tgctctcaaa
     6661 taacatttaa caaggacctg aagactagtt ccattaagtt acatcagtct ccttgatgaa
     6721 ctagaagaag cgttgtcaat atttaaattg taggaccgga agttgaaaaa atggggctcg
     6781 tagcgcagac cgttcaattc ggtaaacatg ataacgggcg tacaatggat agcgcggacg
     6841 cctcctaagc gtcaggtcga gggttcaaat ccctccgagc ccgcgtcaat cttgtccatt
     6901 tatctaggcc cattttgtat tattacaatg aaacatgtta tccactccta tcgctgagga
     6961 tccaatggcc gtttttctat atgcattgaa gtctccagaa tcaaaaaggc agtatccgaa
     7021 acggttcaag atgtttcttg atttcctggg ttttggcggc gtcttgcaag agcaagtgaa
     7081 taaattccga aggaatgcaa aaggaaatcc ggaatggatt cagaatagtc tgattcaatt
     7141 catatcttat cagaatgaca gggcaaaacg tggcgagata tcaatatcca caatacaaaa
     7201 ttactataga gctacgaagc tattttgcga aatgaacgat attgtcttag gctggaaaaa
     7261 aatcgctcgt ggtatgggcc gggtaagaaa gtccgcgaat gatagagcac cgacttttga
     7321 agaaatacag catctcattg aatatcctga tagacgtata aagtcgattg tatatactat
     7381 ggtttcttca ggaataagaa caggagcatg ggattattta caatagtacc acatacttat
     7441 tgacaagaaa ttaagattaa tactaagaag atggtagtaa ggacgtataa tcagtaataa
     7501 gagaaaaagc atgttgctgg caacatagac atcttagtca atgaagatcg ccgccacaat
     7561 aacaataatt ttatttgttt gtacagatga tagagcacct atacgattgg gctagggacc
     7621 gctaaattcc actgctgtct gatttcttaa tttctatggt aattttatcg cccactctta
     7681 tggccatatc ctcgtactcc ctcatactga ttttgaatgt atgggaatcg gtcggggggc
     7741 cgcctccgcc catggcatta gaaatatttg ctattgcatt tggcaaattc ttcatcatgt
     7801 cctctgggga agtaaatgcc atcatattcg taccaaaagg attttgcggc ttttcggcgc
     7861 ctggcttgta atcatttgga tcagcaaatg tgacataaac gtaagggctt ccgtcttgag
     7921 aggcttcaat tcttgaaatg acacagtctt tcttcattta ctatcgcatt atactttgaa
     7981 tgaatagact tataattttt ccagaatgcc cgtttctgat tttagatagg acttgtccga
     8041 gctgaatgac ttcattctat tcgatttggc gaatataggg atgccgaaag ccttgatata
     8101 tacactgctt aatgataatc cgtcgtcaga ttggcgcggg aaaaacaata acgctaaaca
     8161 atactgtgta attagcattc gctttttttg tatggacgat atatcagtac ttgaatggat
     8221 catactgcat tcccgagggc gtacttcaca ggtccccggc tatctctgta attcaatcaa
     8281 tgaaattatt gctttatttg gtgccctcaa atctcttatt attgtggcct aatgctgtaa
     8341 cgtgtacgtt attggaacac ccgaattggc ttgtattcct gtgaaattgc gaacgaaatg
     8401 tttgattagg tttgcgtacc attagcacca aaacataaca gttgaccttc tacatgattg
     8461 actcctactt tgaacggctc gcttgttgta cctttccatg ttgtatatgt tccagcagga
     8521 ccgagaggac taacgggttt gtatccgtgt tgattcgttt gtacttgaac gttacatttt
     8581 gtatgggttg cgtttgatgg cattgatgtc cctgttacca ggagaaagga acctgccggt
     8641 actttttggc cttggtttgg cgtatttatt cgcaagattg aggccattgc tgttggaatc
     8701 gacaagattg atatcgttac taccgtcgtt accgttattg caaataatag aatcgttatt
     8761 tgcattattt acttgttcag catatactag ataaatcttt accgctaatt ttaaccagag
     8821 ctatcccggc attggacact cattagctat taggacccaa aaaaaagaaa aaagatattg
     8881 ttgttagtgt gcggcgagcc tcaatgaagg gcttaattaa tgacaacgga tgagtaccat
     8941 tattctggca tgttccgctg cttgcgttgc taatttgacc ggtggtttga attatctttg
     9001 atgataataa tgggataaga aagccacctg cagataacgg tctaaaatat gatgttgata
     9061 ttgccatcat aaatggacta tctgtttctg attctcttag ttctgctgac ctcaaaagaa
     9121 caatagaaaa aaccctgagc ggagtatatt tcccaagacc tattataatc acttgaaaat
     9181 gctggtaagt gataatctcc taaacagaga tgattctgca ggaattgaca agctagtttt
     9241 ttattcatta acgagagaag ctacatttcc ggcaatgacc tattgtgcta aaagtaccgg
     9301 gaacaatatc gagaataaag cgggctccct tgtcgagagg acagtgatgc tattgttatt
     9361 atcaagaaag cgagttatga catactctaa ttcgtgctgc gatagacttt atgggagcta
     9421 gtttagcagg agtcgaattt ttatcaagat tagcaattat aagatgtggt acattctttt
     9481 tgctaaagct aaatcgaaca cgaaaaagaa aaggaagatc aactatcgat aacgctactt
     9541 gaattcttct tccagtatcc ttgttcttct gcttatttgg aataatgtgt ctgttttctt
     9601 aattgcctgc tctctagaac gtccaaagac catcgattgc agcagcaaat gattttcagg
     9661 gataaccata cctgtctggt cgtctgtgta taaaatcttg agaacatcat tttgaactct
     9721 taacgcttct tgatggatat gtaccatgtt tgtatcacca tattgaaaac ccaggttcaa
     9781 agctttcttt cttacgtcgg cagttccttt ggcggatttt aatattgcca ctccaaattc
     9841 cttgctgaat gcttcaataa tagtatcctt agcaggagcc tttgttttga atcggattaa
     9901 catttgatgg agatgcatca tcctggatgg gactttgtat gcctctacga acaggtttgc
     9961 tgctggcata aaatctttaa catcctcttg atgatgagga gattcatccc attcgattga
    10021 atctcgcacc tctttcgaat cctcaagatc tgcccatctt ctgacgatgg ttacgtcgta
    10081 cctttcaatt tcatccccaa acttttcagc caggggctgc attattctgc ccatacctga
    10141 cacattacaa ctaccttgga taacatacat tttaccgctg gcctttgcgt aatttaccct
    10201 cgaattatga atcatatccg ctaccgagtg ggcaccgtgt ctatcttctc ccccttgaaa
    10261 gatagctgct tttttcatag gctcatatag tctctttttg ttgtcgaacc ctccgccttc
    10321 tttagccgca tctacaacta tgtcgctttg ctctacagct tcttccacag ttccagatat
    10381 ctcatagtta ctatctctga attgtttcag attatgtttg gggacatata catcgaattt
    10441 gctgtctagc gcctctcttg tcttctcgtc ggcggtatat ttcgcgacgc cgaggaattg
    10501 aatttctttg tctgcagaga gcatagtcgc tagacgacgc ccaatattgc catacccatt
    10561 cacaaaaact ttaaccaatc tacttcacta acagctaatt tttcagatga atgtggtttt
    10621 caacatttta tatatatttc ctgccactag cgagcgcata tttttatgta cgcatatcgc
    10681 atttttttac tcaataaatc ggtattgcac aaaatcaata atacaccaca agattgcttt
    10741 ttagcagcgc ccatcttgaa ctaggtttaa atataatttc aggagtacgt acggcagcga
    10801 aaattgtgtt ttgactaggt acgctttcga tttgaggtca atataaaatg agggtccttg
    10861 tcgtagataa ttatgattct tttgtatata atattgcaca acgactaggc gaattaaaga
    10921 ccgaaccgac agtcattcga aatgagaagg ttaccgttaa ttcggtcaaa attatggatc
    10981 ctgatgcgat agtcatttct cctggcccag gccaccccgc agacaggaga tactttggcg
    11041 tatgtacgga cattatagct gagcttggac cacaaacacc catccttgga gtctgccttg
    11101 gacaccaagg aatagtgcac gcattcggcg ggaaagtagt caatgccaga aaagtcagac
    11161 acggaaaaac aagtattatt caatacaact ccggaagact ttttgatcag gtaagcaacc
    11221 catttaaggc aacccgctac cattctttgg tggctgataa gaaaattccc ccttgtctgg
    11281 aagttacagc aacggcgctt gacgatggtg aaattatggc gatcagacac agacaatact
    11341 tgatagaagg ggtccaattc catcccgaat ctgttttaac ggaacaagga ccgaaaattc
    11401 ttctcaattt catctctatg gtgaagagat gagtgaacgg agagacattc accgatctgc
    11461 gccccatggt ccggaaacta gtgtctagaa ttaatctatc tgaagacgaa atccgcaaat
    11521 caatgaatct gattcttgga ggcgacgctt cggagtcgtc cattgcctca ttcttggtcg
    11581 cgcttcggat gaagggcgaa tcgcctaagg aaattacgac gatacttcag gctgtcaaga
    11641 ggaatgctat taagattaca ccaaacatca ccggcgaatt gattgataca tgcgggaccg
    11701 gaggtgataa gataaaatca ttcaacatca gtaccgctgc tgcgctcata gcgtgcgcag
    11761 caggctgtaa agtagcaaaa cacggaaata gatctgtctc gggattctgt ggcagtgcag
    11821 attttttaga atttattgga atggacttgg atacttctcc tgatatcata tgtgaggcca
    11881 tcgagaatat aggttttggt tttctttatg ctgcaaaatt ccatccgacg atgcgcaatg
    11941 ttgcgtctgc aagagaatcc ataggcataa gaactgcttt taatattgta ggaccattaa
    12001 gcaatccctg cacaaatctt tccggtcaac ttataggagt ttatgagccc gcattactcg
    12061 aaacagttgc tgttgcaatg caaaattctg agcttgatga actgatggtc gtacattctt
    12121 acgatggttt tgatgagctt tccaacacct gtgaaaacga tattatccgg gtagttgaca
    12181 aaaaaattaa acgatttcga tttcatccaa aggatgtaaa tgtagtagtt gctaagccag
    12241 aacaactgtt aatacattct ggacaagaat caataagaga tacactccga gttatatatg
    12301 gcctcgctag ccccgaaaaa gaagacatag ccgcgctgaa cgctgcggcc gcgctagtga
    12361 tcgggaaaat tgcgaaggat ctaaaagaag gcgtcgagat cgcacgagca gcgttaaagg
    12421 ctggtacccc ccaaaaaaaa ctatcagaat taatacattt atgtggtaac aaggataaat
    12481 taattgaagc tgaacaagag ttccttatga tatagtttat ttctctatat cataactggg
    12541 ctgctttaag tatcggtttt ctgtagtgct cgatcaaaat tcctgctatt gtgatagcct
    12601 ttcccgaata catttatttc agtcggtgcc taatgaaggg atgaaaagat ataactagtg
    12661 tgatagatat aataagaatc agtataggta ttcacagttg ttggataaca aaaataaaaa
    12721 ttggagtaga cgaacatttc atcctactcc ttctactgca aacaaatagt tcatactaag
    12781 cgcctttgtt acttcctctt gctggtcgat tttgctttta cttttgcggt tcttgtagtt
    12841 ggtgtcttgc tagctgcttt ggccttagca atttttttgc caccaccgcc tatcttgaac
    12901 atctttgtac cacatacggt gcatattcct gagatagcgc cttgaccgtt tttcatagtt
    12961 acctgctttt catctttcat atctcgtttt gctttacatt tcacacaata agcttgtgtt
    13021 gccaacttga cataaatcct cgtgcttgaa tataagaata gctgtatttt ggtttcttac
    13081 tgggacaaaa tctaactgta ttgtgcccat tatgagaaat attcattccg attattgacg
    13141 aagaggtgtc agccgagttt caactccagt tctgcctggt ttccaagctt tgtaagaaag
    13201 atatgtccga ggaactggac ccaagaatag gaccgtcaca ttgcttttcc agtatatttt
    13261 tatgtcatgg cttcccaacc atatgtagca aggtaagcga cccggtttga gctctgtaga
    13321 tcaaaatcca gttcacgatc tagaatcact gaactgggat gacttactgt ctttgaaacg
    13381 tagtcagctt aacaagataa aggacttgac tgacaaaatt atcgatatcg aaaaaaatcg
    13441 atttcggctg ataaatgaga atatccagca tgagaaaaat aaggtcgtta acatgacaac
    13501 aagattggcc cagattcgga caggcatgaa ttctagcaac tctcaattac ttacgattag
    13561 tgaaaaaatt tcaaagtcca aaaattttgt tagtataatg ggaactcggc ttccctccga
    13621 caatgaagtt gatctggtta ggattcttga gtcaagccag aaattagttg atgaaaaaag
    13681 gtacaagaat gaacgacaaa agaatgaagc cttatccgta atgaacgatg cttccatgaa
    13741 gcttgaagca ataaaagcca tacgcactgt gaacgaacag ttaattgatc tcaacgcaca
    13801 agctgaagag attaaaaaaa tactaaaaat tcttgagaac gaagtcacta cgctacagac
    13861 caaaattgct gatactcaca ataaaataga caaactattt gtgtctaaac gacagcaagc
    13921 tgcggaacac caaagctgcc ttaaggatta tgacgaattc ctttcgactt tagatagcgt
    13981 aaattcgcgt ctagatgcta tggcacagtg gaggaaaagg cagcgtcagg aatatggtta
    14041 tcgacctgga gacgatgccc tgttcaaagt taaggaatcc gcaaagaaga agtttgaggc
    14101 tggtgccaaa ttaagctttg aagagctaaa gttgctgtac gatgaagaca agtcgagtga
    14161 aggatgagtg tcctaccgaa tcgccaaacg gcaatgctag aagtaggatg tctatgtatg
    14221 atcctgaatg tcgagacata aatcaggttc aaagccgaaa cacacgatat actgctcact
    14281 acttctttgt ctacttgctt tgggcttatt aatataaact cgttgaaagt gatttttcaa
    14341 ttcctccttc aatccattaa gtaactctcc ttcgaaaact ttgaaaactg aggtgccgcg
    14401 tcttttcagt atcttaatag ccgcagccaa ggcactcctt gtgagggaga tttgtcgact
    14461 atgatccaag tcccatatac cgcttacgtt tggagacaga tctgataaaa ctatatctgc
    14521 cctacccccg actaatttga gaactgtatc tattatttta ccgtcatcga tgctacctcg
    14581 caaaatcgtg acgccttcca atggctgtac ttgcttggta tctatcccga ctaccaagct
    14641 gtcaacacca acttgttgtt ttgctacttg aagccaccct cccggtgcgg agccgagatc
    14701 cactacagca tgtcgtggcc taaaaatacc gtaagagcgg tttagctgaa gcagtttgta
    14761 tgcagatcta ctacgatagc ccgcattttt cgcgagcttt cgataataat ctcttgttgc
    14821 atctgtcaac ttcatccttt atgccatctc tgtagcaata aaggatggcg aggtatacaa
    14881 cataattcaa ctgagccgaa cataacacaa tttcacaaca tgtgtccaca ctagcaataa
    14941 acctcgtatc ctatacagcc gctattgtac aagccctata tgcgtatcta tctagttagc
    15001 attaacaggg tctgtcataa tgctatctag ttagcattat tataacgatc tgccatactc
    15061 taaggctttt cagacttgat tacatttata ggcattctgt tccatccgag gcaagtgttc
    15121 cacccttctc cttggggggt cttcaacata ttttccatca aaatggtggc acatataaaa
    15181 cctttttctt ccttggtctg tcaccatgct ccataaaatc tgcaatagta atgatttaag
    15241 agagaattct atgttcaact tctcaattaa taatagcgaa atactagttg gaagatgcta
    15301 tgttgcataa ctgagtcttc tgttagtatg gtttgaccta
//
LOCUS       DAOR01000132           48030 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_9833, whole genome shotgun sequence.
ACCESSION   DAOR01000132 DAOR01000000
VERSION     DAOR01000132.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 48030)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 48030)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..48030
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_9833"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    23037..23294
                     /estimated_length=258
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        13961 a         9797 c         9521 g        14493 t
ORIGIN      
        1 cagagaatgt aaaaatcgat tctttatttc gacaagaata tgaattacta gcgcttagca
       61 gtttcgtctg taaagtggaa ggaaattgat aaagaaagaa atggtcctta tttcatttct
      121 gaagcaattt ttaggtatgc ctcgcatttt ctttttttga aatttatact taaccaggaa
      181 acaaacttgt cctcaaattt tttacctttt aaattatcaa tttgatctga attaagctca
      241 tacaactttt ttacaaacag tccggagagg aataacttgg caaaatacca tcctgcacta
      301 ggggtcggag agtctagaac gaaattgcaa ttatcatcgt tgtcataatt gcaattcttc
      361 tgaaactcgt atagtacctc ttttgcaatt ttactgtcgg attcaaaatc gacagttaca
      421 aagtctataa gaaaagcatc gatcaatggc atagattctt tactatgcag ccgtgctgtc
      481 ttcgttttcg tacttcagtt tatcagaaaa ggaaactaac ttaccatgat cctcaactct
      541 gatacttgta ttaaccctga tattgatccc tacggaccgg aacaatgcca acaattctcc
      601 tcctgatatc ctttggttaa tctcgcctgc tttgattaat tccacaattt tccttactat
      661 cgccctcgtt tgaattggga actgagattt tgcaagatcc agtacttcct cgcccctgtc
      721 gtacaggttg ctagatatga tttccatgtc tgttcgcggt tgactttcct gttctttcct
      781 tgctttcatc ttctcctgtg tggcagcttg cttctgtagc tctttcattt ttcgagcctt
      841 gataattgtg agatctcgat cttcttcctc tgacattaac cctttatcac ccctaatggg
      901 attaatctta ctactttggt tgctattccg agcgagtcag agacgtctac cacggcatca
      961 acgtctttat atgcatcggg tgtttcttca actattccct ccttcgttaa tcccttgaca
     1021 taaatgcccc ttgattctaa cttggttctc acctcttcaa cagtatgact tctttttgca
     1081 gcagatcttg acattgttct gcctgctcca tgtgcagttg atccaaaact caaatcaaga
     1141 gactttttat tacctataag aatccaactt gcagttccca tagatcctgg aataattaca
     1201 ggctgcccta tttcacggta ttttgcaggg atttcgtgca tgcctgcagg aaaggctctg
     1261 gtagcaccct ttctatgaat aactagatcg cgcattcctt ctccattgat cgaatatcgt
     1321 tccactttgg ctatattatg ggagacatca taaacaagtt tcatatctag ctgctgttca
     1381 ctcataccaa acactttttc aaatgccttt cttgtccaat gagttatcat ctccctgttg
     1441 caccatgcaa agtttaaagc agaattcatt gcccttcgat aattctcgcc ttctggtgat
     1501 tgattaggca cgcacgccag ttctctgtca atcaattgag taccgtactt tttcaaagca
     1561 atttcggaaa tacgtaaata gtcgctgcag acctgatgtc cgaatcctct cgagccgcaa
     1621 tgaattaaaa cagtcaactg gccttgttcg tcaatgccca tagcttttgc agctctttca
     1681 ttaacgattt tgtcaacttt ctgtatttcg agaaaatgat tacctgatcc taagctacct
     1741 aattgtggtg cgccacgttt tttagcgata tctgaaacgc tagctggctc tgctcctatc
     1801 atttttcctc cttcttcaca cacctctgca tcctgttccc gtccgtatcc gcgccctatt
     1861 gcccagtcta cgccttctac aagaagttca tcgagctcag ctttggtcaa tttaactctt
     1921 ccttcactcc ccactcccaa cggaatagac ctaaagagct catttactag atcttttaac
     1981 ctgggtttta gatctctttc agtcaaactc gttttaatta accttactcc acagtttata
     2041 tcatatccta ctccaccagg gctaatcact ccctcttcaa ggtcggtagc ggcgactcca
     2101 ccgacgggga atccatagcc ttcatgtccg tctggaagaa cgacaacatg tttctttacc
     2161 ccaggcaatg ttgaaacatt tactgcctga tgaatagttc tgtcaatcgt catcttcgaa
     2221 ataagatcgt cgttagcata aatcgtaaca ggcaccttca tgtcattaga accatccatt
     2281 tctactcgga actcaaactc attaatctta gtgactttgt ggttaccgat tttggttact
     2341 aattcattca tagtcgtctg tggatttggg gtggagtcat tataagcctt gcttagataa
     2401 tttactggca agaaccaggc aaattatttg tcgtttccca ataaggttta tttttcttgt
     2461 cttcggcgga cttgtgtata tgccaattct tccgattgat acaggtcgct acgggagtcc
     2521 tcaaatcaag cgaatattcg aagaaagaga ccgattacaa tatcagctag agtttgaagc
     2581 cgcggtggca aaggcacagg ctcaaatcaa tatgattcct gtaggtgttg caaaggaaat
     2641 tgtcaaaata gcgaagtctg acaagattac tttagaacga gtgggtaaat tagaagcaat
     2701 tagcgagcat gatacagctg cagttgtgga agccttaagc gagaagtgtt cggcgaaatc
     2761 gaagccgtgg attcactacg gtctaacgag caatgacatc atagatacta ccatatcgat
     2821 gcagatgaga gatgtctttc gcattattga acccaaaatt ttgagacttg ccttaatttt
     2881 ggctgaccgc tcagtcgaat ttcgggcaca gcccgcagtt ggaagaacac atggacagca
     2941 tacaagtatt atcgcatttg gattgaaatt tgcggtctgg gctgcagaga tggcaactca
     3001 cattgaaaga attgaagaag gaaaaaagcg cttcttgctt tgcaagacac taggggtcgt
     3061 gggtaccggt tctttaatgg ggaagagagc tttagaggtg caaaaaatag tagcaaagaa
     3121 cctgagtttg catcccgtgg atgcggcgac tcaggtaatt ccgcgtgaac gaattgcaga
     3181 gatacaattt ttgatcacgc ttgttggctg cacactcgac aaaattgcga ccgaagttag
     3241 gaatttacaa agaaccgagt taggtgaggt tgcagaacat tttagaaaag gtcagatggg
     3301 aagcagcgca gttcctgtta aacgcaatcc tattagaagt gagaaagttt cttcgcttgc
     3361 aaggcttctc cgatctctaa ttgatgttgc attggagaat atcccgttat ggcatgaaag
     3421 agatctatca aattcggcga atgaacgatt taccattcca atggctgcaa ttctgctaga
     3481 tgaaatgttg aattcattaa tgagtgtcgt cgcagaacta aaaataaata cccagcgtct
     3541 cacctcgaac ttggacatga cgaaaggtca gatttattca gaatttgttc tggatgcgtt
     3601 ggtcaaaaaa ggagtcccta ggattacggc atacagggat atccaaaggg tagcattttc
     3661 ggcattgcag agcggacagc atttccgtga tgccatcaaa agggattccc atttagcaag
     3721 gaaactgtcg tcagaagagt taaagtatat atttgaacca aataaccatc tcggcgcctc
     3781 atcagagatc attgatcatg tcgtgaataa ggtaaggaat acaagaaaca aatattttga
     3841 tcataaatct aaactttcgc tatgaatcaa tatttgcaat tttgaatgtt tttaagtctt
     3901 tgaaattctt gtggattagc gaaccgtact gcatagcctt cttgggcttc tctgcagctt
     3961 cgtggggaac accaatcatt acagcaacct ccctgaggat gtgtttccga atgaaatcat
     4021 cgcaaccctt gatcttatta tcaattgaaa tctccagcgc aactgagata aatttttctg
     4081 acagaaaagg gtgcttggtc tttatgccaa actctgctgt tatcgattcc acctccctca
     4141 tgaggatgat ctcattttca atcttttcat gtattaattg cataattctc gcattaccat
     4201 atttgtaaat tgatctaaat ctatcgtaac cacaaaaaag ctcatcacaa ccatttgcag
     4261 ttaaaattaa tcgtaaacca ttttgtcgtg cgagcattcc aagataaaaa aatgctatgc
     4321 aattttcaat atgggagata ttcccacagc ctaatttctg cgatatatat ctctgtttat
     4381 tgtggaaatc ctcttttttt aactccaaca acttatgagg taaacccaac cttgtcgcaa
     4441 ttacctttga aaattcaata tctagagaac caggaaatcc gatagttaat agtgtgactt
     4501 taattcctat atcattgcag agttttgcaa gcaagctact gtcaatacca ccagaaaagg
     4561 cgactgctaa agaagattct tcgtttacag tcgctgtcac agcatcgact aaagcgtttt
     4621 ttaagtccac gactaacaca atctgtaact tctaagatag tctagttcat agatgacttt
     4681 attaaatttt ggagtattgt ataaaatcct ccatcctcag cattccaaag atactgcaat
     4741 gtggattttc caacttttga ataaacacta caatccttgt gcattcattt tggttgttga
     4801 agttgacccg tcccgtactg tacttgatag ccgctcacat ctgctttccg ttcgtctgtt
     4861 ccacagccct cactctccta ctctctgttt cttcgctata agcaatgcct tctattattt
     4921 tgagtgtaaa gtggtagtca atagcaagcc ttcactactt agccaacctg agacatagaa
     4981 taaaatcatt tcagacagca gtgatacact gataatatgt tggagccgat ccaggacatg
     5041 atttcatcat cgattttata aattgcagct gtattatttt tagtcattca gttgccaaat
     5101 ctatctttct gaagtcatcc attactctat tcccacgtta ctgtcgttaa tgtgatactc
     5161 tacggctttg tcaggattgt tagatcactt tgtttagccc tgtccaaaga tagtaggaat
     5221 atataagatc aaaatatata atctaatgct tggcattaga tattgatcag tacatcttat
     5281 tcacaattta tcctgccgcg gctattttcg gactcggatt ttttgcaagg aaggcaaaga
     5341 taggtgaatc gtggaaatat gtgttccagg cctcagccag tattatattt tcaataattt
     5401 attttgaagc cgtaccaaat ggaggggcgc aaggccttgc cgcagtatta ttcctctttg
     5461 gcatactctt gttcttcatg gcgcgaaagt atatgataca tccggaaacg aaaggacacg
     5521 aggaacggac tgccgcttca gccaacgaat cctagagttc gcaccaaact acttgaggag
     5581 cgagttggat aggagaccgc cacttaccac gacgagatct aattctcagt cgcccccgcc
     5641 gcaaatctaa attgaacatc gagataaaat caacttctga acgatgatcg attagagaat
     5701 caataaacta tcgttgtata ttttggtatt ctaatgatgc cgtcccagaa gaagaattgt
     5761 cttagttatt ctccaatatt ttcataataa atgcgtgaaa tatctcgggg ttctttgtat
     5821 aaaattgatt cattagtatg gaataataca atttttttat actatagcat tagtgtgcaa
     5881 aaatctaata gcaactgctt ataaaacatg tttctatcag tccctcgatg tattcaaaaa
     5941 caagagcagc aatcgttagt agtctggctg ttgtagcagt agcaatgatt tttgcaacag
     6001 gtccacttgt tgccaatcac gcatttgcct acggctcaca ccacagctac catcacagct
     6061 atcatcacca cagccatcac cactattacc accaccatca tcacagccat catcaccaca
     6121 actactgtac gagctacgac tacaagcacc actatcacaa ctgctacaag cactagtacc
     6181 acagcgaccg ataacgcaaa gcaaaagatg atttaatcac aaccttttat ttttttcatg
     6241 ctcgcttggt aactgcattc tgacgttaca aaatttacgg atacctatgc ttacattgaa
     6301 tgatgatttc tgacaagaaa agttcctgcc agcaggaacc aattaaattg agtcatatga
     6361 aaaagttaca agtttggtca caagttctct aaagggataa tccaacattc tcttcggttc
     6421 gtttgtattg ggatctacta cgaattggtt gtacatccta ttaacatagt cctgagtgat
     6481 tgccttttct cttggtttga cccgaaatcc tatcgttatc atgtcttctt gtttttctac
     6541 accctgcccc ttagagggga cttgtgctgg tttgctgcca tggggcggtc ctaattcggt
     6601 aaaatctata ccaacgttat tactcactaa gggataaatt cgataacctg accttacgat
     6661 attaagttta atgcggcatt ttttgtctac ccacatcctc cagggaggtc aaattacaac
     6721 tttttgtaga ttcttctata acacccaaga gggttcattc gcacgctcat aggtaacatt
     6781 aggttttaag tttttgtgta aaaatgtacg aaaatttacg ctgccgccct cccgggaggg
     6841 tacgctggcc ctctaaacaa gttgtattca gataacaaac tatatgaagt tcgccactgc
     6901 aaggcaaata actttgcaga aaactagcga aatcaagagt attcgcgatc gaggatatta
     6961 ctaacaacaa taatgaatac gcataagaat caaattgaca tttcgctcaa tgggactaga
     7021 attcaataca gaaatattca tgggatagaa cccactccag gatatcgaat tattccctgc
     7081 cagggaattt gagatggcga ttgttgcagt ttgtggcctt cctcccggga ggaaggccac
     7141 aaagatgtcg atattggcgt acagattgac aaagattgtt aggattctcc cctgatgagc
     7201 gagtatcttt tacagtaacg catagacatt cataaccagc gttgtgtaag aaacagatgg
     7261 gaattcaaag ttagacgaaa ataaaggatt aatctctata tataacgaat ttattggcag
     7321 atgaaacgta aactatcccc gccacctcag tttctgcata ggactgtcta ttgtcttctc
     7381 tgtaatcacc cgctacaaga gtagcgatgg atagaatagc atctggatga tccaacaccg
     7441 cgtctacaat atattgggtt atcttcttca cgtcatgggt tttctgaatg tttttagaaa
     7501 gatcaattac attttcttca gaggggacat tttcatctac ttttcataaa acaacttggc
     7561 cttcgccttc cccggaggaa tagcgatagt tttatacaaa ttccaaaatc cgtgattagt
     7621 attagcaaac ccttagggac aaatccgccg ttttttattc tatttcgcat taattttact
     7681 ctatttacga aagtattcga ataacaggac acgcaaataa ctatttatct agattgctgc
     7741 ctgaacttaa ttcgaagacg agaaacttac accagaagta ttgaataagc ttatcgtcat
     7801 gatggagtga tttcgagtaa tcgtaaatta atgacatata atccacaatc gaggaattca
     7861 gcaacgaaaa ggagcgcgag agagatagtg accacaaatc gtgcgagata tgttattctt
     7921 gaacgcatgg attgaacatt tgcgctgcct tcatttccag actcgatttg ctttcttgtt
     7981 attatgttat tagttctggt gtgtatcaaa tatgtcaaaa gtgtagcaac agtgaggata
     8041 gttcttagaa agagaatttt gccgtgggtt cttaaaaatg caactatccg cgaagaatac
     8101 ccgcgaatta taaacgcttg taattattat gttatattat ccacgttatc agaagaaagg
     8161 ttttaattca ctcttatatt cagctaccct tggacaaatt atgttcctaa gtcgccgcag
     8221 gattgttctt atcgcagcaa tcgcagcagt tgctatatca attgttttat tgccgacgat
     8281 tcaaactatc actcttccaa ccaatataaa tacggtaagc atagcgcttg ccaaggtaga
     8341 acttgtaaat gtaactggca gtagtttgat caatctaaat gtatttttta gcgttcacaa
     8401 tccgaccgaa aagacattga caacttccgg aatcgattac cagctgttcg cagatggtac
     8461 tctgttaggt caaggatcat tatcctacgc ggatgctcca gttaatggaa gacctcaact
     8521 attctctaac actacaataa tattacaatc gcaattccca attgttaact ctcattctaa
     8581 ttcccagatc ttcaaagtaa tacaaaatcc tgcaacactc aagcagatca aatggaaagc
     8641 cgaaggtgta gtagatctgg aatccggagt tagttcagca tcaaaacagt ttagcactgt
     8701 tctttaatct tattcagtag aacagacaca gcttagatta ccacagcctg tcgtataaac
     8761 ggactgtaaa cactgcactt gtagaaatca tcatgaggtc tagggcgtag gctaacacta
     8821 acgcactcag acaattccaa gagatatttg cggacaaaaa ccaactttga tttcatttag
     8881 aatatactat atagactata tagagtctac aatatactat gaggggacgt ttataaagtc
     8941 atataccgaa ctactaccaa tatgtgacaa tttgtgagaa tttcaccaaa tgagggtact
     9001 gaaaagacag aaagtcttgt atggtattga aaatactacc aaaacaatat taggattttt
     9061 atcaagagca aagctgggat atgtacgctg atcatacgtg gccatctgga tcaatgggaa
     9121 ttgatttgtt taagaaagca ttacaagata ttaaaagaag aagagtaaag tcaaggtaca
     9181 tcgtggaaat taccaaagat aacttacctt attgcaaaga gctgatgaaa attgatgaac
     9241 ttcgtcattt gcaaggaatc aaaggtaact ttgctattag tgaaagagaa tacatagctt
     9301 ctgcaacgct gtaagaagca acattattgc aacaaataat ctatagtaat gtaagagcaa
     9361 ttttagaaca gcataaatat ctatttgaaa ctctctggaa taaagcaata ccggcggacc
     9421 aaaaaataaa agaaattgaa gaaggggtta ttcctagctt tatagatact attgcagatc
     9481 ccaacggaat tcaaaagact attttcgatc ttatccaaac ggctgatcat gaaatattga
     9541 taaccttccc accagctaat acttttcgac gaccgtcaag aacgcttggg agtaataaaa
     9601 ttattagaag aagcatcggt gaaacgtagt gtagccatca gaatcagaaa tatgtaacat
     9661 taaagttttt agaagaagga catgaagaag tagaaaaagg atcacagaaa caacagattg
     9721 agattagata tcttgaacaa tctttgcaaa ccaagatcac agccattgca ctagccgacg
     9781 gctacaccgg aaattattat tccatctgtt ctcaacgtgt gaaattccag ttggaagaca
     9841 tgtactgtat ttacagttcc agtatatgga ttcgtatata tggtgcggtc accgggattt
     9901 gaactcccgc tctaccttta acaaggtaga gtcccgggtt acgggctgtc ctcgtattca
     9961 tggacatgaa atacctggaa ggccagtgtc ctgaccaaac tagacgatga ccgcacacct
    10021 acgtccaagt agccgagcat atcgtagata ttaaatctta acgatttaat ctgactacta
    10081 ggtatgcaca atcgctattt ttcagggaat atgtggttta gaagcatatt gattgcttta
    10141 tcttttgctt tctttttgta atactcagaa ttttttctcg gagcttcaaa cgccctttct
    10201 tgttcaactt cgtggacagg tctgtgatct aaagctccct cgaattgaac tctatatccc
    10261 ttaaacttgg atactacaat agagtcgtaa atatctcttt gccaaacaga gccgacgaaa
    10321 tagatatggg gaggattaat ccgcgattta atcatatcta gaactcggtc aaaagtttgg
    10381 gcaatattct ttgggctgga gtctatcaca attatttttt cgtcaactcc ttcttttagc
    10441 aatgcttctt taaccactcc cacagattct gggtttgcga caacgactat tgtagtttta
    10501 tgcttatctg gcttcgaacg tgtaataatc ctcagggtgt ccagacatac ttttacccgt
    10561 tcgagcatgt aatcaggtaa gcttcgtcca tctgtgggtc tttctagata tattgcataa
    10621 agtatgacta gattgccctc cgtaaatcgg tgttccgaca taatgtctat tgcgggtcat
    10681 catatgtttc acatttatat attacccgta gatgctagca tggcattaaa cgatctattc
    10741 tttgttgata gacgaaagct gcggatgttt agtggagggc taaaaaatta tgatgttgat
    10801 gaaaacctcg gactcaaaaa gtaccatgaa ttattctgtt tttcgataag aactaatttc
    10861 tattggagca aataagagct ctttccttgc gagaaaaaga ttaattgtat atctacttca
    10921 acctttaata ctaccaagaa gcgacttttt ttggagcgga aacttgcctt tccaactcgt
    10981 ctaaacgttg tactgtctca ggattcccgc gtaactcaga aaaagatttg tcgtatctga
    11041 ttctttgcat gaccgccaga acatcttcga ttgtcacacc atgttttatg tcgtattcta
    11101 tctgcttagc gcagctcgaa atagtaccgt attttttgaa attacgcttt tcaaaagatg
    11161 acgcatcggg agatatgcct ttgatataac ggatgataat cttttcgaca tgttctaaat
    11221 gccagtcttt catggcaaaa ttgttgggtt tcattaggcg tgtccctctc aaccttatat
    11281 taccaaaaaa ggatatttaa attgaagtat aatagaataa tcacgttatc agctaatgta
    11341 tcactatgct catattctgc tccgactttc atccaagatc agcctctaat gtggatgcaa
    11401 taaaagtata taacaggtga tggcgtacat ccaatggcat gcaattcgac tacgaattat
    11461 tagatgatgt aaatctcaaa attttggaca agttaggcga agattcgtca acaccattcg
    11521 tagaagttgc aaaacaaata ggcgtatctg acgcgacaat acacataagg gtccgccgga
    11581 tggttgatgc aggaataatc aacaagttta cgctgtctgt tgataatgat cgacttggat
    11641 atgatcatct ggcattcatg ggtatcaaca ctgaacctgg ttctatagaa agcgtaattc
    11701 ttgacctttg cttcgtggat gaggtattag aggttcatga actgcatggt accttcgacc
    11761 tgatattgaa gatccgtgct aagaatttag aacagatgag agatattgtt gaaaataaaa
    11821 tccgcacact accacatatt ctagaaactg aattgatgcc tgtactgaaa tcaagaaaag
    11881 aggaacaaat agtctcgtta aaaaaggaca ttgctaccaa ggcagagtct gcagagcgag
    11941 cggcgcttcg gtaaataaca agaactagat ctagaagcaa atttctaaca acgaatttcg
    12001 tatttgcaca aatactataa taggtattca tccaagtgca cccatatcgc tctgtgctcg
    12061 agaaccattc aatcaattac agtaaattcg ggtgatatct atagcattgt gagcctctca
    12121 ttttacggac tagcattcct tcctgaaaca gtataaagca ttacttgttc tctgctcatc
    12181 gggttttcga ttgccgattc tataactttt ttcagcatta ctaatatttt ttatgcgtct
    12241 tttatgtaca atgattttta tgagttcggc agagtataaa ggggaaatta ctttgtctaa
    12301 aatttagacc ggaaagttct tgtgccactt gtgtttcgga gtacgatgta ttctatcttt
    12361 taaggaaatt ctcgactttc tccccaaacc tttactttct agctttctgg ttccctcatt
    12421 ccggcgcatc agcactaaag ttgtatggac tgaaatatgt atgtctatgt agataaatcc
    12481 cattgcaaaa acattttcgg ccaagcagta tactagccca acaatcagtc caaataacga
    12541 tttttgttgt acttattcat atcccagccc gggcggaaaa agtcatgagc aaataagtat
    12601 attagtactt tacaaaactc ttggaatcac gtcaaaggaa aaatgtgtaa aacattgcga
    12661 ctcaatgtaa gacacaagaa gactcttggg tgtttcgcac tatgacttga acaacgatca
    12721 tgtagggtat atcgaagtgg aaaataatac aatattccaa aagtgttaca tctacgaatt
    12781 ctgctgtggt aggctatgga tattgtttgt agccgtagtt tattctaagg gcaggggcga
    12841 tactatcgct acaagcgtat ttagattgac ccttggaaga aagaggaaga atgactaaat
    12901 gaacaaatgc tcccaaagat gttattttag agctgcatat gtataatgat ctatcataca
    12961 tcggcataca tagaaaattc gtgaggatgc ggtctcactg ctacttcatt gtgttctttc
    13021 acttcgtgtt caattatgct atcgatggcg tcgtcgccga agatcggttt caggaatgct
    13081 ctatcactgt ctagcgcctc atacgcttct cttaaacttg caggcagctg cttaacccca
    13141 agttccctac gtctctgtgg ggtcatttta aaaatatctt catcgacggc attacccaat
    13201 ggtttcttct tcagaatacc gtctagtcca gcagcaacca tcgcggagaa tgcaaagtaa
    13261 ggattgcatg agggatccgg ggccctgaac tcgattctct tcatcgcggc aaattttctg
    13321 cctttgtaat gggctgggat cctgattatt gctgatctat tacccctgct ccatgcaata
    13381 tatacaggcg cttcataacc cggtaccaac cttctgtatg agttggtagt cggtgcgaca
    13441 attgcagcca atgcatatgc atggtctatg atccctccac caaaataccg agcagtttct
    13501 gacatttccg catattcgtc tctgtcatca aaaaagaggt tcttctgctt gtcccagagg
    13561 ctaacattaa catgcatgcc tgaaccgtta tcaagtgcaa tcggcttagg catcatcgta
    13621 gcaatcatgt tatttttttt tgcaatattt ttaacaatat acttgtagct ttgaacagaa
    13681 tcggctgcat tcacaagtgt gtcgtaccga atatcaattt cgcattggcc cgctgttgcg
    13741 acttcgtgat gatgagtatc acaaatgata ccaaaatcgt tactgagaac gtctacacat
    13801 tcatttcgat aatccatcag agtgtcaccc ggtgcgcttg gaagatatcc ttctttaagt
    13861 cttagaacat atcctacgcc ctcagtggac cacggcgctt ctcttgaagt tatgttatag
    13921 ctctggccgt gataaggtgt catagtgttt acttcgagtt tatcaaagac aaagaattca
    13981 acttcaggcc cccaaaaaga cgactcatag cctttttctc gaacatattt ggccgccttg
    14041 gttgcgatcc ccctcgggtc gcgcggaaac ggatttctct ctgaaccgcc ggttaggata
    14101 tcacaaatta gccttgctgt ggaatccttt tcaatccacg gaatggtggc gtatgtcgat
    14161 ggatcaggcc taagaattag atctgattcg tggatctctg taaaaccacg gattgaagag
    14221 ccatcgagtt ttggaagacc atcctcgaag tcctctttgt gaaacatgtt agatgccata
    14281 gttgtgtgat ggaatcttcc ggtaaggcca gtaaactgca aatcaagaaa cttgatcctc
    14341 tcattcttta acttttgcat aacttcttcg gctgtaaatg acaaaggttc aatttcgccg
    14401 tcaattactt tatagggcaa ccgcagtatc tgttatgata gcaattaatg tcataattaa
    14461 gctttcaagt cgtatgccat tatcacgacc gattcagcgc tctgtaagca agttgaggtt
    14521 acaaacgtcc tcaaactttc gcgcacccct tacactcagc ttcagaagac caaaagcatt
    14581 cttgtttctt attttatgtc gtagatgccc aaaatgtcaa aatttggact ctctcggatg
    14641 gattctgtag cggcctaaga ttatttattg tcacattgtc ccttgaagtt ctcatccact
    14701 cttcagtctg gaggcatcta gtctgtaagc gtggtctact ttgtcctgcc gtttttttcg
    14761 ttattcaatt tattattact tattgtaaag gaggcatata atacaattaa acgattttgt
    14821 tctaggttga ctgacaaatt ttatttgtta tttcatttct tgttcttccc aagcccgaca
    14881 gattcagagg aagatctatc aattaatagt aggtacaatt attaattgga atttttatag
    14941 tccgcacctg atagatgtga tagcttgagt atgtgaatgc tcatggttga tttctaattg
    15001 ttctaattca tgtctgacta gatgacagac ttcctcgtaa ttttattcaa tttttcctat
    15061 ataaaatgca aaggcaaatg caaaaattgt accggctatc ctgtctctac ttttcttatg
    15121 gattattatc tttataaaga tagattgctt ctcatttata caaatgctta ccatgccaaa
    15181 tgcgtttcat tgttctaatg cagtacaagt tgttaaactg tctatcattg tttgccgaac
    15241 ttttctatga tggcggccat gcatttagtt gccttgttaa ggagatcaat tcttgcgaat
    15301 tcattcggtg aatggattcg tgcataaata gaggtgcttc ctacagagac acatggagca
    15361 ccgagcattt taacaaatga atgcatgggt ccagttccag aggatgagac gctaataata
    15421 ggttctgttc caaaagtttc cgtcgcggcc tcttcaacct gttttacaaa aggttgattg
    15481 aaaggtgttc tagatgacga ttctccatga ataaatttta tatcaatatc accgccaaac
    15541 ccataattgt ttaaatgatt aattagtctc tcgaattgtc ttgtggggtc catatcgggg
    15601 accaacctaa aatcaatttt agccatagca gtcgaaggaa gaacagtttt tgcaccttga
    15661 ccgacatacc ctgctacaag gccagcaata ttacatgttg gcatacccac taaagccttc
    15721 cttgcttcaa ttcctcgcga gtcgtttacg aatttgtcga ccccatactg ccttttgaat
    15781 tcctgttcat caaacggttc tcgagaaata ctatccaatt cgtccgaggt aaattccctt
    15841 acctcttcgt accaatcctt gatcaaaatt tttccatccc tgtttcttat gatgtttaat
    15901 gcttcgacca gacgccatgc aggattttca atcaatactg ccaagctaga atgaacatct
    15961 tgtgaaggtc cttttgcgac tagctccaca taaagtagcc ccttcatccc taggcttata
    16021 attggtttat ttttttcgtc aacatatccg aattcccaaa ttaccccgtc ggctgcaagt
    16081 ttctgtctgt atttggaaag gtacttctct atgttcacgc tcccaatttc ctcttcaccc
    16141 tctacgatga attttatatt acagggtaca tcacctgttt tttttagaaa atactctaca
    16201 gccttaatac gggttattag ctcgccttta tcgtctgcag agcctcgccc gtagatataa
    16261 ttaccctcca ccttgccacc aaatgggtct gtatcccaga attctacagg ctcttctggt
    16321 tggacatcat aatggttata aaacaataga gtcttcgcgt ccggattcat cttcgattta
    16381 gcttcaccgt aaactattgg agcgatagcc tcttgattct gatttgagtc atgacgatcg
    16441 agattcaaca cttccgattt tattcctgct ttgaccataa tctgggatac taagctagca
    16501 cattcattca agccctgctt ttttgctgaa atactgggtt gtctgattaa ggtttgaagg
    16561 tcagaaatga ggcctttcat ttccgagtcc accgcggcac taacactctc gttcaacaat
    16621 ctgcagcttt acagaatcaa gttaaatgct ttctagttcg tttatctcca accttataca
    16681 ttgatattat agacaattag gctcagtaga gcaacatgca tttataacca tcatgtcttt
    16741 tttctatttt ctatatgtct tcgtcgtcgt cattgactag ggatgagaag cgggtatttc
    16801 tttctccgca atccatcgct gttatcggtg cctctgagaa ggcaggagtc gggaatgcta
    16861 tattttctaa catagtaaac ggatacaaag gaaaaatcta tccgatccat ccttccagta
    16921 gtacggtgtt aggcatcaag gcttacaaat ctgtcctcga tgtgccagaa gacatcgatc
    16981 tcgccgtggt tgcaactcct aacagaattg tgcctgccgt aatggaagat ataggcaaaa
    17041 aaaagattaa gggtgcaatt attgtgactg ctggatttaa agaagttgat gaacagggtg
    17101 caagattgga aaaagatatt gagtcaattg cgaaaagcta tggaactcga atcataggtc
    17161 caaattgtct cggaattatg agtttatctg aaagcaatat tatgaatcta acatttttaa
    17221 aaattacccc aaagtatggt ggaatagcgc ttttatctca gagtggcgca atttgtgcag
    17281 cgactgtgga agacgcgata gaacaaggaa tcggcttttc aaaagtgata agtatgggga
    17341 acaaagtgga tatggatgaa aatgatattc tcgaattatt agctgacgat gccgacacca
    17401 aagttatcgt gatgtatctt gaagacattc acgacggaag gcgatttatg gaaattgcaa
    17461 agagaatcat aatggaaaac aagaaaccga tcatagtgct aaaagcggga cgaactcctg
    17521 aaggggcaaa ggccgcgatc tcccacacag gtgcactcat gggccctgat gaaatttatg
    17581 atgccctttt tgttcaatct tgcgtaatta gagtagatac aatgcaagag ctttttgaac
    17641 tttccactgc attttcaaaa cagcccacgc cccccgagca aggcggagtt gtaatagttt
    17701 ctaatgctgg tggccctgcc atcatctcta ctgatgcgtg ctcaaagtac ggcttgaaaa
    17761 tggcagatat atcatcatcg agagaagcaa ttgctaaggt aataccgcca catgggtcgg
    17821 caagaaatcc aatagatata gtgggagatg ccgactttaa tcggtttgag aaagtccttg
    17881 ttgaagtgat atcaaatccg agtgtcggat cgatagttac gatgtgtaca ccctcagcta
    17941 ctttagatta tgacgatctg gctagaataa tcgttaagac atcaaaaggt agtgggaaga
    18001 caatgcttgc tgcattaatg gggcttgcag agggtactaa aaataaggaa attctctccg
    18061 agggagggat tccacacttc atgtacgctg aatctgctat ccaaactcta gaaggaatgc
    18121 atcgcttcag agattggtta aaaagatccg ccggtagacc aaaggcattc aacgcagata
    18181 agaaaaaagt aaggacactt tttgataata tgaggaagtc tgggagaatg aacttggttg
    18241 aagaggaagg ttatgaagtc ctggcagcat atggtttcgc tactccaaaa accgttctcg
    18301 cggatagcgc agagcgctgt gtagatgctt ctaacaatat cggttatccg gtcgtaatga
    18361 agatcgcatc tcaagacatt atccacaaat ctgacgcagg aggggtcaaa gttgggcttg
    18421 caaatgatga acaagtgcgg acagcgttta atgctatatt gcagaatgcg aagacgtaca
    18481 aaccagacgc gaacattaaa ggagtactag ttcaggagat ggtaagatct ggcaaagaaa
    18541 taatccttgg tgccaagcag gatccaatat ttggtccact tgttatgttc ggtcttggtg
    18601 ggatatatgt agaagttctc agagatgttg tctttagact ggctcctata gatgagaacg
    18661 aggccagtcg aatggtccac tcaataaaaa cacttaacct gttgaagggt gtcagggggg
    18721 aaaaaccctc ggatcttgga gccctagtgg atagcctgca gcgcctttct caattggtga
    18781 ctgaatttcc tgagatcgaa gaatttgata taaatccgct gctggtatta gaagaaggca
    18841 aaggagtacg gacagtagat gttagaataa gcctcaggag ttaacccaac ttctttccgc
    18901 attcttcaca gaaaacggca ccctcttcgt tctggtagcc acatgatgcg cattgggagg
    18961 gactattggg ggcagaagta cgcactcttt caccgaggag tacgatgtct ccaattttcg
    19021 aaatattctt ccacaaaact tcttctgtca ccacagtctc agaattgttg tagctattct
    19081 cctgcttgga gatttttata ctaatatcca tgtttcctct tggcgacttg cgtagtccaa
    19141 tttctaaaac cctgcccact aacatagctt cgctatcata tgcagtcatg tcaactaaag
    19201 attgaattga aatgaatcca ccctgtgcgg cttctgttgt tgctgttgtc gtcgcggctg
    19261 cctctggtcc tactcccgtt gctattggtg cttgaggact attagtatca atctcgctca
    19321 ttgattcttg cgcgtaagta gctgtgctct ttttctcatt attagaacca gaatctcgtt
    19381 gttgtgcttg taactgttta attgatgctg acgtatctgt tgactcttca ggagaattga
    19441 atttgcggta ttgagctatc gtattgttgc atcccgtgca cctgtactcc cctttttcaa
    19501 gtaaactgac tccttcaccg aggtcttcat taggtttttc ataattaatc gttggagaac
    19561 cttgaaattc tttttcgcat ttgttacaat aatattttga aaacctatcc tcattcagtc
    19621 tacccactcc tgcaagaaac agatcaggac ctccaaggtt gcccttcttt tgttcctcgt
    19681 cagttacttt cgcaaaaacg tgaccgccag agccacgcaa tttcttcacg gataagtcgt
    19741 cgccttcaat catcatttat gtatacaatc tctaagacga aacaatcgta ttagtatttc
    19801 gtatttgtta agttacattg cggaatctgc acgtagattg ttcacagacc atatccaaaa
    19861 ggaacaaaac taatatgatg agcctatcgg ctttttcttt tggcgaggtt tttatgaaag
    19921 aaaaaaatta tcatcttttt atcgttgcat atttcatatt cgactgtctt atacgattcc
    19981 tagctgccta ttcccacgga agcttaaaaa cacctgttgt cggccttttt atactgtaac
    20041 cctaaaacgt taatgtatat acaatcagat gatatatctg gcgttgaagg ttatgaatta
    20101 gtttggctac aatcagataa gaaggttcac ttactcttaa catctggtta catacatcgg
    20161 taaattattt atctccgtat accttatatt tgtgttattt atataaaatg cgagcaagag
    20221 atatacggcg gatctgttaa gtaaatagaa aattgcttct cgtgtcttcc gtgacttttg
    20281 taacaacaca tgtgagagga caaagaaatt gaaaactttc aaagagttag ggatgagtac
    20341 ggagatagtt agagctctcg aggaaaacgg atttgacatg ccttttccta ttcaagagac
    20401 agccatacca tttattttaa aaggtatcga tgttataggc caggcacaca caggaacagg
    20461 taagacagcg gctttctctt taccaatatt aagcagatta aagcgcaacg ggccagtaca
    20521 agccctgata ttagcaccga caagagagtt agcagttcag ataaccaccg aaattaataa
    20581 gtttgcaaaa tatatcaaca ttaaagcagc atccatatac ggtggacaaa gcatacacct
    20641 ccaactggat caattaaaga ggggagtcca aatagtagtt gcaactcctg gcaggttgat
    20701 tgaccaccta aaacgcggtt caatccggct agatgatgtg aggtttgtag tgcttgacga
    20761 agccgacagg atgttggaca tgggcttcat cgatgatata aagttcatct tgtcttatgt
    20821 aagtgaagat agggagacgt gtcttttttc tgcgacgatg ccccccgaga ttttgagact
    20881 agcgcaagac tatatgaaag aacctcaaga gatcagatta aatgaagagg atctgagcct
    20941 tgatacaatc gatcaatctt atttgattgt ccctgagacg gagaaattta agcatctatg
    21001 taactttata aaaaaaaggg atcaaaaaca gactatagtt tttgccgcca cgaagcaaag
    21061 gacacagaga ctcgcagcgg aattgaaaca ggaaggattc aaagcaataa caattcacgg
    21121 tgatctttct caaaaacaga gagatatcgc aatgtacagg tttaagaagg gaaatgaaga
    21181 cattctggtt gctacagaca tagctgcaag aggaatagac gtcccagcca ttggtcatgt
    21241 gataaactat gatattcctg aggatccatt gatgtacttc catagaattg gaagaactgc
    21301 gagagctgga gcaacaggca gagctatctc attagtttca caggacaggg ttgaaaattt
    21361 tggaagaatt cttagacaaa cgcagcaccg catccgcaaa cttaacgaag agatgggaat
    21421 ctcagttcct gtcgtcaggc agcagcaaca gcggcgtcgg tatcatggta caagccagcc
    21481 acgacaaggg tatggtggag gcagtaggtc ccgggccagc tttggataca agagggggta
    21541 tggtgataga agacatatga gcagcaatgg caacagttat cgtggtagca gcagcgatgc
    21601 ttggggtaga cgcggcaaat ttgagaaaac aagataatcc atcgcctgga tgcgaatcta
    21661 tacatattag agtggcattt taatacagtc ggtctgtaat tgcggcttgg acgcaaggaa
    21721 aagataatct aatatatgaa aaattttatt tagtgaaata aacgcaatgc taaagcatga
    21781 tgcaataatt gcctagaaac ctcatgttag attaaaatcc ctctgtatcg catactatat
    21841 atccaaaaat agcaatacat atgtatgtac atgccctcgg ctgaatacga agattctttt
    21901 gttaaaattg caggtctgat tgggggagaa gagtacctaa aggttgctcg tggacttcta
    21961 aatacagatg atgccacaga tgaagagatt gcaagtgcca ctggtctgag gattaatatc
    22021 ataaggaaaa tcctttatga tatgtttggt aaggctctaa tcacaggaat cagagtaaag
    22081 gacgaaaaaa aaggctggtt cgtatataga tggagagcaa aacaagatca ggtagatagt
    22141 tttattgata atcaaaagaa aaagatcctt gacagattgc agatccgttt ggagtatgaa
    22201 gagtcttcgg agttctacca ctgcggaaat aaagattgtc cgcgcttgaa gtttgattcg
    22261 gcggtagagt tgttctttaa atgtacaatt tgcaaagggc cgcttaatat ggttgacaac
    22321 ggtagagtaa aagaagcact tcgctacaag atcgagcagc taactgccga tatatcttta
    22381 agaaagcaat agactttttt ggtactagcg tctgcctagt ctctaccaca acagttttgg
    22441 gttcagttgt ttgcgagaac aagtgaagcc aagtagttaa atattactca gcgctattat
    22501 aaaaccaaat ggtaaataaa tactctgttg caagaacaac agaagaagaa gctggtgaag
    22561 tagtcggtat ctgggaggta ttaggaagaa gatggtcttt gttaatctta aagaatctct
    22621 gcaccaaaga agtaatacgt ttcaatgaat tcaaaaaact cttgtcaggg attagtagta
    22681 ctgttttggc tgataggtta ttggagctcg aacgcgaagg ccttgtatct aaaaagattt
    22741 acccacagat tccaccaaag gttgaatata gtctaactat tcgggcgaaa gagctaggag
    22801 taatactaag agaattggga aaatgggcta gccggtggaa ggtacctaga attacaacga
    22861 acacaaaaac gacaagtgct gctagatcag ctagttagtg atgggacagt attttaaggg
    22921 tttttttact gaccaacgat ctaaccatga aactttgtga ttaattagtt ttagatattt
    22981 ccccaattaa tgactgccac gccagcggca aacttgggtt caattgagat tcgttgnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnattgag attcgttgac tattggatat acagatcttt tttgttgctt
    23341 tattgcagag gttctagcaa attaggtatt tgggataggt gtagagtaag aatttgcatc
    23401 ctttcattaa gcttagaaaa gaaagactca atactgtact ggtcattctg ttacctacta
    23461 ttactttgtg agattgcagt aactactgta cgggaagatg tcgccctagc agcgagtgct
    23521 aatatattaa cgataaccat gtgaagatag aatctaatcc tactggtttg attcggtcta
    23581 cttcatctcc tttccactag taccagttac cgtcgaagta catctacatc ttttcccaat
    23641 attgcgtttt tcagccttta tctatatcat cgaaactatc tactggaaca ttgctatggg
    23701 tcattagcag ctttgttttc ttaccccaca tcaagagggc tcaattcgta tgtgaggtga
    23761 gagaggtgac tttcaagcca gcctgattca cgccattttt gaaatcagct cgtcttttct
    23821 aagttcaatt gtgctgccgg atagatatca gtcataaaga gaaaatgcac cgccaattac
    23881 gccgtcaatc tctgcattga ttccagtaaa ctctgcgtgt ttcgttgttt ccatgagcgc
    23941 ttcaaaccca tcttttgggg ctacattgac tgtaactttt tgtttgatgt tcttaacttc
    24001 catattggtg cacagagtaa cctaagattt aaatatttat ataatatatt aattataata
    24061 atattgatta atatgggatc tccgtggaat gcaatagcag atgacaatag gagagaaatc
    24121 ctcctattgc ttaagaaaaa agagttaatt cccactgaaa tagcatttta gcttcacact
    24181 accagaagta tcatcacatc tccgcatatt gaaggatgca ggattgacta cagagaaaaa
    24241 agaaggcaaa aatattgttc attaaataaa agcaaaacgt tggaaatagt acaattcttt
    24301 gagcacatgt acggttacag cttgaattca ctaaaggaat atgtagaaaa caaagaaaat
    24361 aaaatatcta atgggagtag gaagtggtga tagtagtcga tgctgcgcca gaagtagttc
    24421 tcaaacttat aactgacaat gacgagttga ctaaatggtt tcctgttaat gcgatacttg
    24481 gaccgaaagt tggagatcag gtagagttct cattcttcaa ggaaaactct gagagacgaa
    24541 atagagattt tttccctgaa ggtaaagtcg tagaatatat tccaaacaaa aagctagcat
    24601 atacttggca acagtcagat atgccagatt tcaccagaac cgtagtaacg tgggaactag
    24661 gaactagacg agattgacaa gaataagaca agggtcgagc tgtgcacttt gggttcaccc
    24721 taaaaaggat acaagttcca tcgcatgccg caaatgcttg ctgaattatt gttggcaaga
    24781 aacttgcaac tcgtattcct actgcttaaa ctgtaggcct tgtgaatatt gttagtaata
    24841 acttacgaat actagtctac ggttacctat aatactagat tcagcatgga ttttagaaaa
    24901 gctttaagat atttggttcc taactgagtt tgttgcagag gaaatggaca ctttcgaatg
    24961 tataataacc aagctagacg taagagaatt cagtaatcag gatgttccat ctgaaataag
    25021 gtcaaaggtg cttgaggcgg caaggttaac aggcagtggc cttaatactc aacactggcg
    25081 atttatccta gtgaacgata aacagaatct caagaagttg gcagaagata gtacaagcgg
    25141 aagctgggtt agagatgcca attttgcagt aatagtactg acaaatccag agtacaattt
    25201 tcatttggtt gatgcaggaa gagtgctaca gaatatgcaa ctggccgcat ggaatcacga
    25261 ggtgggctcg gggctgttta caggaataag ggaagaaaaa cttcgaggcg actttggaat
    25321 accaaacgag ttgagtccta caatcatagt aggctttgga tatccggcaa ggaagatgac
    25381 cggtaaaagt aagaagcgtc tgccgctcga tagattagtt tactatgaaa aatatggcag
    25441 ctccaaaagt agtactgctt cagattcagg acgataacct gtcaaaatcg accagctctt
    25501 ctgcgcaatc gagctgtctt cacgatctga tggaacaacg tttctgcagg aagtgagtga
    25561 aaataatgat aattcaagcg tggctcaaag gcgacccaaa attcatttag tttgccaaat
    25621 gagaaaaata tgaaaaaggc acgagtactg acattaagtg cttgaggcca gattgacatt
    25681 aagtacttac aaattaagcc cagatcgcag tcaatctcag ttttgtttct gccaacttca
    25741 aatgtaattt ggggcagcac tgaatatttt tattagatga aattcctcta gcctgccata
    25801 tagagccata agaactaagg caaaagttac agtgtggaat ctggggcaca atcggattta
    25861 aaaggagaac tcaaaacagg ttactattat ttagaaaatt atagagatta tgctcgtagt
    25921 tgccaggaag acacctcatc ctgagtctaa agctcgtaaa acaagactag ctccccgtta
    25981 ttttgtctga gtactttttt caattccatg cctattgcct gcgaaatttt ttcataccca
    26041 tctccttcga ctagtgtcgt tgctgatcta ccacccacta atttcggcga tactgtcaca
    26101 attagctggt cgacaactcc taggtcaaga agagaccaat ttagctcacc gcccccttcc
    26161 gcaagaatgt tcttaaatcc catctttttc agacatgata atgcttctcc gagatcaacc
    26221 atttcccttc ccgcaataat tactattgca cccgtactcc tgagtttatg gattttgttg
    26281 tcatgtgctc gttctgatac aacgactatc gttgtgaccc tgtgagcagt tttcaagatt
    26341 ctcgagtcta gtggtatcct accgtcgcta tctacaacta ccctagcagg atcttttcct
    26401 ttgttagatc tgattattcg gactgtaagc aacggatcat ctattaggac ggtcgaaatt
    26461 cctactataa tcgcatcgac cgatgagcgc aattcgtgga ctcttattag atcttgtttg
    26521 gaagaaattc ttgaatttcc acaagtcgtt gcaattttgc cgtcaaccgt cattgctgca
    26581 ttaattgtta ttcgcaaatt cgaattgtgc tccgtctaca aaccttccat ctatcatgac
    26641 cgccttgatc gaagcctcgg tagctctgtg aactatagta gcgaatggat tatgtatagg
    26701 gtacagatct acatggcttt tatcaataaa aataatatct gctgcccggc cagactgaat
    26761 ataccccgaa tttagtctga gaatttcggc gttatttact gttgccatct ttagtatatc
    26821 cctggcactc agaaagatac gttcgacaga ccgtgacacc ttccagatat aatctaactc
    26881 ccgtaaaata tccggagaat tcaacatcac gttgtcagtc ccaataccga tgatgcaacc
    26941 taggtctaac attttcgcga ccctcgggac gccagccccc acaatcccat ttgctctagg
    27001 acaaacaact attcctgttc gatttctcgc taccattgaa atatcctttt cagtggcatg
    27061 cgtcatgtga ataataatat caggcttcag acacttcatt attcgagcaa cttctgtcct
    27121 accggtatat gatttcgaaa gttgcatggt gttctcagat tcagcggcat gaatcgctgt
    27181 tagcatcttc ttcttcttat ctttcgttgc acttgtgttc ctggctcgaa ctaaatttct
    27241 atactgttgc agtgtttcgt cgctattttc gtttgctccg ctgattccca aaccatcaga
    27301 aatttctaaa acatcggaag ccatctgcaa tgaactggaa ggaagatttc gttggctggc
    27361 gccggtggca gatgatgtta tatcaacata atactcggtt cgaccaaata cgatacattt
    27421 gatgggtaat ccagaaattg catctcttaa taatttgact ccctcagtac caccctcgcg
    27481 aaagtctgcg aaggtagtta tccccttttt catcatagac aacgcagaac ttctgataaa
    27541 tgtctttaaa tgatccggtt tacttctttg taatattttt tgttttgctc catgtattgg
    27601 atgaactctt gcatctagtc ctgaatctac tgcaacatct tttcccagtg aatctcctat
    27661 atgtgtgtga gcgttgataa gaccggggat tatgagaaat ccttctgcat caaatattgt
    27721 attgttgtgt gcttccttgt cttcgccgcg atggtatggt ccacgttttg cttgttttat
    27781 tataccatct ttcccaatct ccagataacc ctctgcaaca aaagttaagt ctttgccgag
    27841 aagcagacta acattcttga gaattagaga cattctactt cataaactgg tagatccttc
    27901 ccgacatgtc ggaggtctct aatcgtgtca agtgcttttg tagcggcgtt ggcagctttt
    27961 ctcccagtcc ttccaatccc tatcccacaa tttagtttga tattcgtgcc ttgagtaacc
    28021 ttcattatag tctcctctgc atcttgtttt gtagcagcat ttgatacaac cataaaattg
    28081 tctcctccca gaaagaaagt caacgattct ttcttaacaa attcctctga gagcctagcg
    28141 tagatcttga taatcagaca cgtcatttca tagggagaca gcttggagcc tatttcagca
    28201 gaattattca tatcgatatg cattatttgc acaacttcat tgcctcttgg tatcgagttc
    28261 gacattgaga attgcaccgc cgcgctcgcg aaaattcttg ctttgtcggt acctagttct
    28321 cccattttcc gggtgttgta tgcgtccaaa tttgcttgat atggcgtttc gccattccca
    28381 atagccatag acagcttgag ttttttataa agactggaaa gttcactctc gattactaga
    28441 tgatccgcaa ctgatagacc attagtaatt gcaaaatact catcaaatct atttaaataa
    28501 acaagacaat ccttcgccga aaatagtcgt tgcaaatcgt aatagaaatt tgcctgaagc
    28561 atctgcagcc gcgcttccct gtcgctacca agagtaatag tccaaggacc atattcttcg
    28621 attctaataa ttgtgagctg tattgtcaac gtattatttt catcttacct gcaagccttg
    28681 agttctcttc tcttattttt ttaattctcg ttgccatatt aactgcgcta atgactgcgc
    28741 ggatgggaac aatttcgctt cggtcttttg cttgtttaaa gctcatatta ggaccggtaa
    28801 tccccagtgc gactggcttg ctatgtttaa cggacaaatc agcaatcaat ctggcagcat
    28861 tttctgcgac tatctcatca tggttagtct cgcctttgat tactgcgccc agggtaactg
    28921 cagcatccac gtcttttttt ttcagaagct cttggactat caccggcata tcgtatgacc
    28981 cgggaacata gcatacataa gtgattattg catccatctt cttcgcttgc ttttttgcgc
    29041 taccgaacat tgggaaagta atctctctgt taaactctga aactactact cctattctaa
    29101 ctttatccaa ttataaatta tgacacctta gcatacgtct tggtacacgt ctttctttag
    29161 ttgaattgat tctagtaagg gaatgttatt cttgcttgca tatttttttg ccttctttat
    29221 cgatagagcc ctatgcgtag tagaatccaa catttcgcaa atcgctacgg caggaactag
    29281 gccggcaata ttcattagat atacgcataa ttcagtatga cccgttctcc cgtaaagcaa
    29341 gccttttgag gctattaaga tcgggacatg accaggagca cggaaattgt ttgaaaattc
    29401 atcaactcca gttgaatcaa tttttttaca aacttcagcc atcttcatga ttgtcaatgc
    29461 tctgtcaatg tcagtaatac cggtgtaagt gtcgcgatgg ttaactgata tcgaaaaact
    29521 agggttgtcg ccgtatggcg atctccctga cgttagtttc ttaaataccg gattgagcct
    29581 tgacagatct ttaatgatct catgcatata gactagcccc aatttcactg caatttcatt
    29641 cgcaactgct aagcagataa ggcctcctgc gattcttctg aatgttgaaa tatgatccgg
    29701 attaatctgc tctgcagcta tcaccatatc gatctcgtct tctcgtccca tttcgtcgtg
    29761 gacgagtacg aatcttccct ttcttaaagc ggtgatagct ccctcaactg aatccaaatg
    29821 gttaatctgc ggctcagatc gattatatag tttactaaaa ttcgagtatt tactatttta
    29881 ttggtagatg tctcgataag tacttgccaa taatatctgt ctcgacgtta actttttccc
    29941 ctatcgattt taatgtgagt gtagttgtag ccagtgtatg cggaatcaac gccacagaga
    30001 tcatcttatc tttatctttg atatctatta ttgtaagact gactccgtct atcgctattg
    30061 aaccttttgg cactatccaa gacattagtt ttttattctg gattcttatc cagaccttcg
    30121 tctggattgg cgatttaatg atcttcttaa ttatgcctat tccgtccaca tggccaagga
    30181 ggaaatgtcc atccaatctg tcaccgagtc ttaagctcct ttcaatgttt actttgtccc
    30241 cagcttttaa taatccaaga cacgtgcgat tcatagtttc atttattatt tcaaagtcga
    30301 cagtcttcga tatccttgta gccgttagac aggttccatt aatacttagg ctatcgccaa
    30361 cttttaaatc tcccttgagt tttcctccca gatttatgca cagtttggta tcagccgatt
    30421 tgttgttgct tctgatttta gagatcgatt tgacttctgc aagaccctgt atgataccgg
    30481 tgaacaaagt gtatatctct aactcagcat cacgtgtgct tagttaaaaa atatactgca
    30541 tattgaaaga ttatggaagt gtttgagcgt taaaactata cgccttattt ggaaccactt
    30601 ggaatacata taattttcct tttatcggtg atcgataaat gtgtctgggt agttcgtcgg
    30661 gatgaaggga tctatagact cgaaagcatt tggggtttga atctacgtga atttagaatc
    30721 cctcgttcag tagataatct gatcccagtg tgatttttgt tttctggttc ctagaaaaaa
    30781 tatacttaac aaaagcatca ttggttgaca atatccaaaa tggccttcgc ttgcttataa
    30841 tgcgctgcgt caatcatttt tccctctagc tttactacgc caccgccttt tccctttcgc
    30901 atcgccgggc ccaaaacgtt tactacttta gtagcccatc caatttcgat tttgctcggc
    30961 ttgaaaattt catgtacagg gtctatatgg tttgggtgaa ttatgctctt tcctacgtag
    31021 cccaatcgtt tcgcacttgc ggcatcattc cttagtccag gaatatcatc gaccttctgc
    31081 cagatggcat cgatcgctac caccccggca gctctggcat caacgggaat tttccaccgt
    31141 gcatacgaat atccagttcc atcgttgtct gcatgatcga ggtgcatgtc atggaggaag
    31201 tcataaagcc cgaagattaa ggcgtttact cttaagtcag ccctagcaat cgagtgagcg
    31261 ttcactaccc ctttagcagt ttctatagat ggcattaatt ttatacggtt cttttcaatt
    31321 cgcctctttt gctccaactc agtaataagg ctacacagtt ccactatttc gctttcctca
    31381 cttaccttgg gcacaactat gccgtcgagc cctttctgaa ttgtagatgt taagtcttta
    31441 cctgaaatac ccgattcagg tgagtttatc ctgacataca cattttctgt taagttataa
    31501 tttgctcttt gttttaacgc ttgtacgagc atctttcttg ctgtgacttt ttcagatgga
    31561 agcacagagt cttctaagtc aaagcaaatg atatcggcat tcaatttctt ggctttatcg
    31621 acaaaccgtg gattaattcc aggaacaaat aatagacttc taaatcttga catcgaaaaa
    31681 tggaaaagag gaaggagaga gattagagtt tctgaggtgt agtcaagatc ttaccaatct
    31741 gttcagcttt ggccatcatt acgtgccctc taaccatgtc gctaaatggt aaaattgtat
    31801 caattacggg ctttatcttg cctctgctcg tccaatggat tacttcttcg agttcggctt
    31861 tagtcccctg tgtcgacccc agcaaatttg ttcctttaaa gaaaagatat ctcaagtcga
    31921 gagtagtatc gtaaccagtc gttgcgcctg ttgcgacaag agttccgcca atctttagaa
    31981 gcgcaactcc ctgtggaaat gtcgctttcc cgatatgttc gaagacaaca tctactccct
    32041 gtttctttgt aatttccctt accttcttag accaatctgc ttctctatga ttaacaacgt
    32101 cgtcggcgcc gatctcgata cacttgtcca ttttttgctt gttgcctgct gtggcaatga
    32161 ctgtacagtt atacagtttt gctatttgaa taccagccat tcctacaccg ctagtaccgc
    32221 ccattatcaa aactgtttga ccgggcctaa tcctggccct ggccactaac atatgccagg
    32281 cagtcatccc aaccattgag accgctgctg cagtttcaaa tgaaacattt tccggtattt
    32341 tggaaacgtt tacctctggt agatgtgtat actgtgcaaa accaccccaa agtgggcctg
    32401 tttggaaacc ccaaatagtt ctatcgttgc agtcatattc ccttcctgcc gtgcacatat
    32461 cgcagactct gcagctcata tttgagtgtg acactgccct atcaccaggc ttgagtttag
    32521 taacgtcctc gccgacttcc acaatggtgc cagcgacatc actgccagaa atatgtggca
    32581 gcggtgtctt gattggctcg ccccagatag cccataagtc attatagtta tatgatgcta
    32641 attctacctt tatcataact tcgtttggtt tcagctttgg catgtcgatg tcttcgattt
    32701 tgatgacttg tgtgggatct ttgctatatt ctcggaatac agctgctttc ataaaagtga
    32761 aagcgttaac gagacttaat atagttaact aaagtgcaag atcttcacgg gttctaaaaa
    32821 atactcgtat acagtactac tatccattcc tacgaaaatt caatagatat agttagaaac
    32881 gatcgaaaaa agaaaaaagt ttaaagccta ttaatcctga aatctttttt tggttgctga
    32941 atactaagca aaatttgctt caattcgtca tctgttaatg ctctattaag tttcccgctg
    33001 gatgccgcat ttaatagata attttccaca gcatttgcaa gttctggttt aaccattctg
    33061 atattcatca ggcgttgacg ggcagaggca tccaacagta tcagcagagc gcgttgtcgc
    33121 attgcggcag cctcggcttc gcgtttcctt gcttcttcgt catttggctg ttggggttga
    33181 ggaatcgaca ttttgcctcc agacctccta ttacatccat ctaaccgtat cgctgtagct
    33241 ccggcgcagc tttgataata tctttataga tttctgtaga taatctatct atcttcttca
    33301 ttccttcatc tgatatcgat cggcctctct ttttcttggt aacataaccg gctgcttcca
    33361 attgttgtaa tgctttgcgg ataattgctc cacctgcgtc tttatgatga tctaaatagt
    33421 accctattcg ttttctgcct ccgtactcgc tcttcaaatc cgcaaccccc tcgggaccat
    33481 gaatgtaaac ctttcttgct atcgatgcgc atctcgcgta ccaccagtct ttgttctggg
    33541 ggatcttttc agcatgtgcc ccagtcttta catacgctgc ccacggagga ggctgaattt
    33601 tcttatcgtt ccgtagttgt tcggctaatc tggttatcag tacagtagcc ggaatatcat
    33661 aagctttagc catagtaatt actgactaat cacgctgtaa atcccttata aatccttata
    33721 ggaagttatt ctgatttaga agttccatgt gcgccctgaa aggattttgt gataagtgtg
    33781 tccacacttc acacatgtaa tccttatact cttcgtgtta gttcgtccta ctctgattct
    33841 tgaatttctt cctgggataa taaacttttt gcacttctta cagaacaatt gtcgaatctc
    33901 ataatccagt ctcacacgta accgcatggc aattttcctc gctagatacg cttgttcatt
    33961 agcaatcttt tcgtttgcat gcgcttcttt taatgcattg gctataagta tgccgaccct
    34021 ctcttttgca atgtccttcg aaatctgcct cctcataggt tgtgctataa aacgtgcttt
    34081 ctcatattaa tatgctagtc catttgttct acgtgatttt cgtctttttt aggcgataaa
    34141 taaaactgta attaactggg aatgttaaat tgtactggtt aggaatggct aatttcgctg
    34201 aactagaaaa gacggctgag aaatacgtga atttaaaacg acaaaaaaaa atggaccaag
    34261 aaagaaagga attagaagaa gagctgaaca atatatcaat cagtattatt ggttatttct
    34321 cctctccaga atttgcattt cctttagaaa gacaagaagt agttagcaat gggacaacaa
    34381 cctatgttta caaaaataat tccacatatc cgaatctttt tgaatttatc tctgagctgc
    34441 tacacacacc gatcccaatt gcggttgaag gcgcaaaatt tggtccaggg gaaatcatag
    34501 ttaacggaga tagcagaaag gccggtcgaa gagagcttgg ccattgtatc atagaactcc
    34561 agaagttaat tattggtaag aagccttaag aaggaacgta cgagtggtca tcaggatgcg
    34621 gctaatatgg gttccacaag agaggaacct aagatactaa cctaagttag ctgaaggaag
    34681 aagacctgca aagatagagg aaaggcatct ctgaacatat gataatctaa gaatactcta
    34741 tcgcataaag gctatgtatt gtaaattaat gacaataggc acctccccag ttgtaaatga
    34801 gattcttact agagatcgtc tggagtgata catcatctta atggttgtca gcttcagcga
    34861 acgagctaag gatctatcat ggttgtcaag gcagctataa tagacagctc aaggctaagt
    34921 cgtacactat tggtaggatc agtggcactc gtaatcgcgg aggcatctct agagactgtt
    34981 cccaaacaaa tcttaaggca tatgtcagta ataaggcatg cccaaaagct tggcaaaaag
    35041 ccgtcagagg tattgttaga tagaagctat catcattccg cgatggtgac tggcagactt
    35101 gaaagcgtat ggaaacgagg ccgtccagac atagttcatt ttgcgttagt ggaagcactg
    35161 tccactccct tatatctgaa gaacaaattg gctgtttatg tctatacgat caacagacaa
    35221 gtgatcttaa taggggcgaa tttgagaata cccaagtcct atttccgatt cgaagggcta
    35281 atgatgaaat tgttcaacga taaagtaata aaaagtgaac aagacgatag aattctttta
    35341 gaattgctag atgacgttcc cttcaaacat ttgattaaaa atatagcaag gtctagtaaa
    35401 tcgataggac tatcgagtgg tggagtttca agcactgctt acgaggtagt ttcaaaatac
    35461 atcgatcgat tcgacgactg tcggtgcgct atcgtgatag ggggcttccc gaaaggtcgc
    35521 ttctctgaca acattagcaa tttcctagac aactcgtact ctattggaaa aataacattg
    35581 gaagcccatg ttgttattgc tagagtactt tacgagtgcg agaagatttt gttcatatct
    35641 taatatgaat atgacctctg gctgtagtat tagtacctgg tacttagaaa tgacgcgggg
    35701 ttgaccagtt ggttggcttt ggaggtgcat agcagagtaa cagacgttta agaaggttga
    35761 accctgactc tgaaaggacc ttttatcaac ggaacaacga gtccataatg aaggcaatga
    35821 acagtgtggc cagatatggg ctggtgactt tgaatactat ccaagatact ctctcgcttg
    35881 gctttaacaa tagccagatc gataacgcta gcataattac accggagata ctcgcggtaa
    35941 ccagataaat catcccgaag tggttgatga agaatggtaa tatgctgaac aataccatca
    36001 ttaatgtagt cccagctatt atgcggactg attttttctc gccagaaact actggtagca
    36061 tcggaacgcc agcctttcta tagtcttccc tgaaatgcaa agcaaggctc caaatgtgag
    36121 ttggaatcca caggaaaacg aggccggcca tgatcaagcc gagttcaata ttttgtgtcg
    36181 tgactgaaac ataacctatc atagcaggga cgccgcctga aaaacctcct aaaataatgt
    36241 ttgttcggct agttctcttc aaccatttac tatacactat aatattatca aacaatccaa
    36301 aacccatcaa tgcaaaagcc cagatattaa taagccatgc aagtaccaag gaaatcgcgg
    36361 cgaggattag accaaatgcc agggcattat ggggagaaat tcttcctgaa ggaataggtc
    36421 tttgcttagt cctgtccatt attgcgtcaa tatctcggtc gttgtaaccg gtgagggtgt
    36481 cagcggatgc tgaaccagca gctactccgc cgatgatcag cacccatgtt accgggggaa
    36541 tgaggatatg agataagaga gaagccgtga gtgccccagc aaaagctgtg aaaacaagca
    36601 ggtaccaaat ctttggcttc gtaagctcgt aataattttt gagcctgttg ccggcgctca
    36661 tttgcgggag cagggtgccc atgtcaaatt tttaactatt ctgttataaa aggcaatatc
    36721 atgacaaatt tatcctccaa gagatctata cctattttga tatagaggtt ggatagatta
    36781 tttcgcgtgc ggtcttcctc atttcaataa atgtttctgt cttttgaact ccctcaacac
    36841 gacccacctt ttcagaaatc aagtggtgca tttcatcaag agactgtgca tgcattgtta
    36901 ctaggatgtc aaatcttcca gtaacttctg cgatttctcg tacctctgga atcctgaaca
    36961 gctcgttgag gacattgtct ctcatctttg aatccatatt aattcctgtg agtgctttga
    37021 cattgaaccc cagagcttcg tcattaacca ctatggtaaa tttcttgatg aggcctcttt
    37081 tcacgagtct ttttattcta ctgtaaacta cggaagcatt aacattgatc tttttcgaaa
    37141 gtttgggtac agaaatactc gcatctgtag caagctcacc caaaattttg agatctaaat
    37201 catccgtttt tcccatacaa tgtcgtaatt atatctctaa ttgagtgact aataaaccta
    37261 ttaatttcaa acttagaagc gttttttgga aaatcatgtg tagctaaagg atcctcctat
    37321 ctttcagtga ttctgcaagc aaaactgcac cctttgccga acccattttc tcattatgtg
    37381 ataacaaaac gtatttcagc ccattgctaa agaccgtatc ctgtctcagt ctacccacca
    37441 ctgtcgtcat cccatcatta atctccctat ctattcgagg ctgcggtcta gtgggatcgt
    37501 catggacaac aataaaatct tttggagctg ttggtaattt tttcactgct acagatttag
    37561 aaaattttaa catctcctct tttgcatctt ctggctcaca atgccttttc gttccaacaa
    37621 agacaacttc tgtatgacca tccaaaaccg ggacccgggt gcatgtacaa cttaccttaa
    37681 atgtagcggg cgtaatagat ctggaattga tgtcacctaa aatcttcttg gattctattt
    37741 gaactttttc ctcttcttta ggaatgtacg gaatgacatt gtccatgata tcgagtgcaa
    37801 taaccccagg cgatctgccc gctccagata aagcttggag cgaagtcata aaaaccatct
    37861 cgactccgaa ctcattcata attggtttta gtgtaatggc aaggcctgtg gtggtgcagt
    37921 ttggcagcgg agcaatgaat ccttgccatc ccctattttt tctttggatg tttagtaact
    37981 cggcgtgctg gtcatttaca ccgggaatca aaattggaac atcctcttca tatctaaagg
    38041 cggcagcagt acttataaca ggaaccgatt ttgcaaattg cggctcaatt acttttgcgt
    38101 cctcagattc caaagcagta aatattagat cgaatctttt tgggcttata tcttcaaccc
    38161 tgctaatgat gacatccctt atgtattctg gaatcgtttc tttgctgtgc cagcgaagaa
    38221 ttccagattt tggatcgcgc agtgcatcca tgtatttttt gccagcagat cgctctgatg
    38281 acactatctg agttaactca aaccatggat gtttatttaa tgcaacaaca aattgctgtc
    38341 caacagcgcc tgtagctcct ataagggcaa cttttagcat acgatggcag gtaatcggta
    38401 tgcagttaat aatctttttc agactgaatt aaatcaaata tacaaaatct ctttaagttc
    38461 tatatgatat gatctaatgg aaactgaaat gataataaat ggaaaaacgt tcaaactaat
    38521 taggaaaaat cgtgatagat ctacgtggtt gcttccatgg aggcatttat tcagtagacc
    38581 ccagcctagt caaaatcgac tttagctcaa atgttaatcc acttggagtc tcgaagattg
    38641 tcctcagctc tattcaaaaa gacctgcgtt cactttcttc atcataccca gatcctgaat
    38701 gcaaggatct gaaaaaaagt ctatcagatt atcttaaaat tcattgtgaa tgcattaatg
    38761 taggaaacgg agcgaccgaa ataatccaca actttgccag aatgtttgca aggggaaaag
    38821 tcaccatccc gtctccgtca ttctgcgaat acgagcttgc atcaaggcga atgggtgcaa
    38881 gccttattct cgtaccactc aagaacttta acttggatgc tgatcaaatt attgaaaaag
    38941 ctaaggggtc tgatgccata tttttatgta atccgaataa ccctactggt ctgctttaca
    39001 gaaagtcact aaaaacgatt atcgaacgcc ttgacagttc gacaaaaata cttgtagacg
    39061 aatgttttat cgaacttgtt aatggatcga atcgacatac tatgattagg aacatagatg
    39121 aatttgataa tctgataatt ttacgatcgc tcacaaaatc ctttggcctt gcgggtctcc
    39181 ggctcggata cagcgtatcc aaccctaggc ttgccaaaaa attggcttct ggtcaaatat
    39241 cttggaacgt aaacgggata gcacagagag ctggcatcgt ggcactcaaa gattcacagc
    39301 atttaacgag agcacgagcg attgtgaaaa aggagcgtga ctttatgtac ggcaacataa
    39361 ggaaaaagac acaatgtttt tcacccttta aatctgatgc aaattatttc ttaattcgtc
    39421 taacaaatca caattccttg acagtaagag attctctttt ggccgaaagt ggagtacttg
    39481 tgagggattg tagtacattc agcggaatgg ggtcaaaata cattcgggta gctatcaaga
    39541 atcacggaga gaatcttatc ttactaaatg ccttggagtc gattgaaagt tgaaagcgag
    39601 gcttttgatg atccaaggaa catcatctgg cgcaggtaaa agtactattg ttatggggtt
    39661 gtgcagaatt ttttctgatc taggatatcg tgtagctcca tttaaggctc agaatatgtc
    39721 ttcgaatata catattatta ccgaaaaatc cgaatctaaa gagatagccc tagcccaggc
    39781 tatccaagcc gaagcttgta gaaaacgacc agacgtaaaa atgaatccta ttttaataaa
    39841 accgctcggc aactaccgga gcagggttat cttgaacggc atcttttact ccgaaatgca
    39901 tgccaaagaa tattatgaaa cattcacctt gcaaaagggt tttccaatcg tcattgaagc
    39961 gctcgaaaac cttagaatag aaaatgatat agtggtaatt gagggagccg gttcaccagc
    40021 cgaaattaac atttcgaagt atgacatagc aaatatgttg cttgcaaaga aagtcgaagc
    40081 accggtaatc attgtgacag acatagaaag aggaggatgt tttgccagca ttgtcggtac
    40141 tctcaagtta ctaaaacctc aatataggag cttaatacgg gggtttctaa taaacaaatt
    40201 ccgtggcgat ccgtcgatcc tagaatcagc aataaaatcc gttgaaaaaa ttacgcgaaa
    40261 aaaaaatttc ggcgtgatac ccaaattagc atttaatttg ccagctgagg attcgcttga
    40321 cggtgcttca aaggttgaaa ttccatcgag agcttggagt agggaaatcg acatcgttgc
    40381 aaactcgata aaaaataaga tctctatcga cgagataatg acaaatgttg tgggtctaga
    40441 taaaatatga tggaggtggg ctacggatat ttattaattg gactaatcgg gggagtttta
    40501 atagatatct tgtttggaga tcctcctaat aagtatcatc ccgtgtcatg gctaggaatt
    40561 cttattggct tctttatacc aaaattaaaa ggagacaaag atggtcacaa tgtgaacaag
    40621 gaaaaactgg gggggatgat tttcgctatc gtattaattg cattaattgg catcgcaacc
    40681 cagtttttag tctctatgtg ttttcatttg tttggattag tcgccgctgc tatcctcacg
    40741 actctaatct tgaaaattgc tcttgccata aagggaatgg aaaaacatgc tattgaaatc
    40801 ataaacgccc tagagagggg cgacattaag agcgcacaac ataatttgtc attgatagta
    40861 agaagacaaa ctaaccagct gagtagacaa gatgtacttt cggctacaat agaatgcata
    40921 ggagaaagta ctgttgacgg gattgttgcg cccctgtttt tctattcaat attaggacca
    40981 gctggagcgc tagtatacag gaccattaat acgcttgatt ccatggtagg ctacaaagat
    41041 gattattaca agtatattgg atggatgtct gctaaattgg ataccatcgc taactatgtt
    41101 cctgctagaa ttacctcgtt tttgatggtt atttcctctc gaatgcttgg cgcggattgg
    41161 aagaattcta ttcagatttt gcgtcgtgac caccgcaaga ctcgtagccc aaatgctgga
    41221 tacccgatgg ccacgatggc tggcgctctt cgaattaagc ttgaaaaagt tgggcactat
    41281 tcactagggg aaaatcgtga gcccttgtct atcgcgaaat gtaagatagc aatctctata
    41341 atgaaattaa caacaattct gttcagcctt agtctttcca ttccgatgtt actaatttta
    41401 tatctggttg gttggtggag acttctattt ggtctttaga tggatacaag caataatttc
    41461 atttctgtca ataataccta ctactagaca ttcagccact atcgacattt attctgtcgc
    41521 caaggtcatg tatctttttc ccatcgctgg agctattatt gggatcatag tggggatgtt
    41581 cgcatatgca atttcgcttt atatacaacc tctcatccta ggggttctgg taacggcagt
    41641 actcgtcata atcacagggg taaatcatac ggatgcgctt gctgattttg ctgatggatt
    41701 aatggctaag ggagggaagg aaggtaaaca caaggcaatg cgtgatccag cagtaggttc
    41761 tgccggaacg gtatcggttg ttctttacgt cgcaggtatg attgttgcac tatctagctt
    41821 ccactttgaa gggttaaagc tgttaaccag tattgtagtt gctgaagcaa ttgctaaata
    41881 tacgatggtg ctgcaggccc acttaggatt atccgcatgg gaaggtttca gttctccgtt
    41941 tactgctagt atgaaggata gacgaaaaat gttagcttcc acagcaatta taattctaat
    42001 tctagtgcta acaggaggcg gatatgcggg gattatgtcg ttaagtggtt ccgtactgat
    42061 tggaataata ttacattaca tttcaaagag aagctttggt ggcatatctg gagatgtcat
    42121 tggcgcatcg aacgagctca ctcgtctttc atctttaatc attttctctt cagcaacaaa
    42181 cgattggtcc aagcaagtac tatgatagct gctttaatgt gtggcgggag aggcaccaga
    42241 atggggatcg cccatgaagc tgaaaaacca ttgttaaaac tgaaggggag cactatgatt
    42301 gaccgggtgc tccacgcatt ggtaggatcc gggcaattcg agaaaatagt agccatcccc
    42361 agcatcaata cgcccaaaac caacacattc atgcgtaatc attattactc ttctttagaa
    42421 ccgatcgatg tagtcgaaac tggcggtatc agctattcaa aagacctttc ggtggctgtc
    42481 tgcaagttca aacctgcaag ggtcttcgta gtatcggcag atcttccact actgaattca
    42541 aaaattgttc aaaacattgt tgtcagatgt ttgcctagta acccatgtgt gtctatcttg
    42601 cttgaaaagc tgtttgttga aagtatgggt atcaaaccta gcatagtgac cgttataaga
    42661 agaataggat attgtcactc agggataact attcttgact cttcaaaagt taaccgcgag
    42721 tttagtttag cggaacatta tatagtgatg aacgagaagg cgctagcagt caatgtaaac
    42781 acaacaagag aattggagat cgccgaggga atgcttgagg acgagaatta gtcaagtatg
    42841 ttcaggactt tgccgagaat gttcgctttg gcgccagcct ttttagcaag cggttggcca
    42901 caagatttac agttaatgtc tgttgttgta tgcgtaaaga taatcctttt ttctccacac
    42961 tcagtacatt ggacagaaat aaaattacta tttggcttcg gaatcagtat atttctttta
    43021 gacatcatta ttatcatcat caccttctta gggttgtatt tccagtttcc gcattcgtat
    43081 gccttccttc atcgtctgtc tttgacaggt tttacaggta tagcgtaatc tgatcttctt
    43141 tgtcgtcttc gcagtcctct tgagctctgg aaacttttgg cctccataac ctcgcttatc
    43201 ctcagcgtgt cggcgtgcac cctgtgcagt ggccctgtcc ttgcccttct tgtaaatgga
    43261 cactgcttgg actgtgtgag tcttgcattt agaacagaat ttgttctgtt ctttctgcat
    43321 tttcatcaga cacaatgcta gtcaagggta atttaaggaa agcgtctccg gataacgtcc
    43381 ggtaattcaa cattgtcata ctatagcgta acatcgttgt cctttataga cggacagatt
    43441 ctatgaacag aaatccaaca gcgtgggtag tttctatgtt tcaattgcca tagtaatgat
    43501 cgtgatctag atgcagcgaa aattcatgca gaggagggta cttaggattg tcagcatttg
    43561 gaccgggccg aatattcgaa aatcctgtgg ggctatgcaa aaaaggtaaa atattgggca
    43621 acatgaaaaa atataatatt tacatgagag attcaaaaaa aattgatttc gaggtacgag
    43681 aaatatccga tacagacttg caaagaggtt ttttcgacac attatcgaat ttgacggagg
    43741 ttggaaaaat cagtgaagat cgcgagaatg caaagaaagt cctacatgaa attagatctt
    43801 atccattcta caaagtcttt gtagcagtta aaaaggacgg cgaaatcatc ggttcaaaca
    43861 ccttattgat agaacagaag tttatacata atggagggag agtggggcat attgaagacg
    43921 ttgcaacgaa aaaaggatat gaaggtatgg gtatcggcag agcgttagtg ggccgatcct
    43981 taaggtttgc tgcacaaatg aaatgttaca aagttattct cgattgctca gaaaagaacg
    44041 tgccgttcta taaaaaaatt ggcttcaaaa agcgtggact ctctatgagg tatgacttac
    44101 tctgaaactc acagggtctt ggtcaaaaaa aagaaacccg aaaccaagat gatatttatt
    44161 atgaaattct agacgacaaa ttggcttctg aagaatatgt tacacgtcat agccttagga
    44221 gagttacgac ctgtactttg tgcctcgtct ggtaattgca ttaaacggcg agtcggttcg
    44281 acgttcattc gagaaaggaa agtcataaaa gatgaacctg acctgtacag tagtaggtcc
    44341 atacgtttct cacaattttt tgaccataac gtaaaccact tttgagaaca agcaatatgg
    44401 ctagccttgt aatcaaaaat tatgcagctt tgctatcgta ctgttcgtgg agtaaatttg
    44461 gtagagatgg ggaatttgta gtagcgattt acctgaactc ctgtggctgg aatgtacagt
    44521 tatctcgagg aagtagggga cctgcagata tcattgcaac cagaagttcg actaaatggc
    44581 taatacaagt aaaatcaagt agaaagattc cgagcctcaa aggtcaggag ctaaatcgat
    44641 tgagagaaat ggctaaaagt gctgatggcc tcccgatagt ggccacggtg cagcctaagg
    44701 agacggccat tgcagtatca acagaagagg tgagaagtgt cgattctaat gaatacacca
    44761 tacagagagg aaaggaggca gggagacgaa gtgaatcgac agcttatgga atatttctct
    44821 actctcttgt agattggaag atagtgtacc cctagacgag aatgtgtcac aattatactt
    44881 ttaccggaag ttactaccag cacgcttata agccaccttt gccatagtcg tctcccgtgt
    44941 atgcacgggt ttccagcatg gatccgaata gtctaggcga gcaatatatg tgtaaatgca
    45001 cgtatgataa tgcctaccta ctctatgctg tgaatctaag gagaatattt tgttgatgca
    45061 atcaactcca actcacagaa ttctaaatta tcttcaagta cgggcaaccg caagcgctga
    45121 gataaatgag aatgtttggc ccgttctgga ggccatcatc actaagtacc ctcaaagtat
    45181 tttacacgct cttttttaaa ttcccgcgag ctgaataaaa tcttataatc tttaatgcca
    45241 atcgactcag acatctggac tgtaattttt tctgctgctt ctattgtacg tgcgtgaacc
    45301 atactgaata aattaaactg ccaatctgga taaactggtc tccgataaca atgactcacc
    45361 tgcggaaaag ctgcgagttt attgccggcc tcgtcgattt ttaaatcagg cactttccat
    45421 actaccatac cattggcagt aaaacctgca tcccgatgac gcaagattgc tgcgaatctt
    45481 cgcattacac caagatgttc atattcttta gcctttgcaa agagttggga ggttgttatg
    45541 ccgagagtgt gggcgagctc cttaaacggt tctgctatta cctttagatc cttctgcagt
    45601 tcacggataa attctttgtc tcgttcggtg agattgaatt tccgtggatt caacgtctta
    45661 acattgtcgg ttggttctgg cattttggca tcttcattaa ccatatccag cctaacacct
    45721 atcttataga gtttcaaagt tggcagcaca cgatatttca taacaccatc tatcgaagcc
    45781 attctctcta aatccatctt tatatccgca ttgggaggaa cagcaagcgt aaaccacatg
    45841 ttgaattcgt gatttctctc ataattatga ctcactcctg ggtgcttgtt tacttcttca
    45901 gcaacgtgat ttagtttgtc aggtttaacc acaaaagcca caagagcact tttataacca
    45961 agtcgccttg tatcaaaaat ggcattaatc tgcctaatta gaccgatttc cttcaagtgg
    46021 gaagttcttt gcataacatc tttttcagaa atattgtaac gttttgatat ctcaagatac
    46081 ggtctatcag ttagcggaaa aacccattgg atttcattca aaagttgctt atctactgta
    46141 tcgatttgtt gtgcctggac tgatgttggt accaaggaat attcgtcatg cttgctcatc
    46201 tgttaaaaac ctatccacct acgatagcac gaattcactt cttaacccac taaataggtc
    46261 ttctctgaga tgttggattt tagaggtctt ttactaagag gattcatccc ttccaacaat
    46321 agcatcatgc gccttcatga ggttcttctt tggttgggtt aacacaagaa aaatatggca
    46381 cttaccaacc cgataactta gtgaatttat gaagatactt gtaactggtg gagcgggttt
    46441 cataggatct aacattgcaa acaccatgag cagagaaaaa aatactgctg tggtcgcatt
    46501 cgatgattta tccttgggaa acccaataaa tctctctcag agtgtaaaat ttgtagaggg
    46561 ttccgttatg gattttgaac taactttaga agcaagtaaa ggatgtgatt acatcttcca
    46621 ctcggccgca aaaagctcgt ccccaatgtt ccaagatgat cccagggaag gaatcgaggt
    46681 aaatgcttta ggatttatga atgtgatgga gtctgcgaaa aggaatcatg ttaaaaaagt
    46741 aatttttgct tcatctagtt ccatgtataa cggcttaccg attccattta aggagtctca
    46801 aactatcgca ccgaagacat tctatgaggc ttctttttat tgtagagaaa ttttagcgaa
    46861 gtcatactat ttggaaaacg gattgaactc aattggtttg agatatttca gtgtttatgg
    46921 tcctaatgaa aagcataaag gaaagtttgc aaataatatt tcgcagtttc tctggaatat
    46981 tacaaacgga gaaagtccaa ctatttatgg cgatggaaca cagacacggg attttacatt
    47041 catagacgat gtagttcaag caaatatttt agcactccag tcggaagaaa aggaatttgg
    47101 gatttacaat ataggcacag gcactgaaac gtcgttttcc aggatagtag aaataattaa
    47161 ccaacgtctt ggaactgata taattgccac atatgtagat aacccaatca aaaactacgt
    47221 tcagaaaacg aaggctgata tatcgcttgc taagtcggaa ctcggatatg aggccaagtg
    47281 taggagcctt gaggacggga tatcagcact gataaaaaaa tcatgctaac accttgtcat
    47341 aacaaaaggc aactatcctc ggattccaca aacatgataa aaactgccga ctctgtacca
    47401 tgttgatacg aaagagcaaa ctcctatttt gggctgtacg ttcgtctcat ttatactacg
    47461 aagaagtagg agaaatagag gtagaagtag atgcagacgc gaaaggcttg atatcaaagt
    47521 tattttcgta gaggcctgtt atttctgcga tatccttctc agcaaggaat ttgccgtctg
    47581 acattcccgc aaacgtctcg agttcctcaa tgtcaatcac tgtaggcaac acgacggcta
    47641 cttctttttg agacagtatg aatttgactg ctaactgtgt tatattccac ccgtttgcca
    47701 gggcaagcgg tttcattttt tcaacctttt gcattgcttc aataatccat tccctttttc
    47761 tgctgctcct atgatcattt ttgtcgaata ccgtcttctc attgacttta cctgttagca
    47821 caccggatgc atcaggaact ctcaccataa ttcctacatt attttttcta gcgacatcaa
    47881 aaaaggttct accagggtcc tgttctaaga tattgtaaac cgtctgcaag cacgtgatat
    47941 ttcggttttc catggcgaac accccttcgt ctttccaacc gatagcagga cctaacgcaa
    48001 caccatgact tctaatcttg ccattgttta
//
LOCUS       DAOR01000133            7740 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_52779, whole genome shotgun sequence.
ACCESSION   DAOR01000133 DAOR01000000
VERSION     DAOR01000133.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_52779"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2513 a         1334 c         1919 g         1974 t
ORIGIN      
        1 ggattattca tagtatttct gctgtgaagt tgtgcgcggt gacatgataa aaattagccg
       61 atgtcgacta agttggcaag caaaactaca aatcaatata atttacttac ctacctaaat
      121 caatctatat tcctctagta aatcgtcaac aaatgtttgg cctcggtgta ttagcaggag
      181 ccgcagatct ttcgactgga aatccgggat tagacttttt ctatactctg ttcaacggca
      241 tatctgaggc tcctttgttc aggtctcatc aatggaattc tgatactcgc agttgtgatt
      301 ttggcatcac aaacggctgg tgtaatgatg gtattgttag gactgatagg tgcaggaatg
      361 gctatcattt ttggcgctcc gtatggtgcg caatatgcct ggtactatga taagggcaaa
      421 ctttcttaca gtcaagacgt caaacaaggt ggacaatttt gcgatcccag tgagaaagaa
      481 cggaacttgt gatgaaatcc atttttggtt gagtgttgtt gtgcttccat taaacctctc
      541 tgcttcaaga taagtactta gtcgactgca ctcaagcttc tttttcaccc taacgaaaag
      601 cggaggaaag atgatagtct aaaaataggc tgaaatccca tttatatact ctaatatctt
      661 gaagtttaaa ttattcggcc tttcgctata gcttgaggta aaagcaaaaa tggtgttaac
      721 gccgagggaa attgataaga tgatgatttg gacagcagca aagatagcag aagatcgaaa
      781 aaagagggga ataaaactga attatcctga atcagttgct tacatagcta attatgttgt
      841 tgaaggggct cgagaaggga agaccgtagc cggattaatg aaatcggcaa gagaaatatt
      901 gaagaaggct gacgtaatgc ccggcataca agatatgata cacacggtgc aagtagaggc
      961 cacgtttcca gatggcacta agctcgtaac agtgcatgac ccggttcaat aggcggttca
     1021 ataggcaatg gttagtaagt caaaaacaaa agtaaaaact aaaactaaaa ctccaactgc
     1081 aaaaaaaaac caaccggaaa ggaggttagt agcaaattga taccgggtga atacatactc
     1141 tcatcgaagc cagtcgtttg caacgaaaat agaaagactg taaagattac cgtcaagaac
     1201 actggtgaca gaccttctca gataggttcg catactcact tctttgaagt aaataaagca
     1261 ctcgacttcc caaggcagaa agcatatggt tacaggctga acataccggc tgggacttca
     1321 gtaagatttg aaccgggaga ttcaaaagat gtagagctct gtgaaattgg gggccatcgc
     1381 attgtttacg gatttaacgc tttaacaatg ggagggttga cttcctcggt ggtgaggaac
     1441 gcagcgtttg acaaggccaa gagacaaggc tacaaagggg cataagagga gagaggataa
     1501 gaaatggttc ttaaattaac gcgaaagcaa tacactgatc tgtatggccc aacaaccgga
     1561 gacaaagtga gggttggaga caccgatctt ctcatagaag tggagaaaga cctgataaca
     1621 ccaggagatg aatgcgtctt tggtggagga aagacgttaa gagatgggat agcacagaca
     1681 ccaggcatta caaatgcgaa tggagctctt gaccttctaa ttacaaacgc cattatagtc
     1741 gatccggtac ttggaatagt caaggctgac atcggcataa aggacggcaa gattgctgga
     1801 attggaaaat caggtaatcc ctatacgatg gacaaagtgg atcgcaatat ggttttttcg
     1861 gcatgcacag aagctactgc aggtgagcac actatctgca cgccaggaca ctttgatacg
     1921 catacacaca tgatatcgcc gcagcagtac atggatgcca tcagtggcgg catatgcaat
     1981 atgataggag gaggaactgg tccggcagat gggactaatg caactacctg tacgcctggt
     2041 gtctttaaca taagtagaat gatggagagc gtagaagata tacccatgaa ttatggattc
     2101 ttgggtaaag gcaatgactc tcatccgtct ctggcaacac tgatggaaca gatagaagca
     2161 ggagtatgtg gtctcaaaga tcatgaagac tggggtacta ctgctgcagt gctagatgca
     2221 tctttgaagg cagcagacgc tactgacact caggtggcca ttcatacaga ttcaattaat
     2281 gaatgcgcgt acgttgagga cacgataaat gcaattgatg gtagagcagt gcacacttat
     2341 catacagaag gtgccggtgg cggtcatgca cctgatatca tgaagattgc gggtgaacaa
     2401 ttcgcacttc catcttcaac aagccctact agaccatata cagtaaatac attagacgaa
     2461 caccttgaca tggtaatgtt ctgtcatcat cttaatcctg ctgtgcctga ggatgtccat
     2521 tttgcagagt ccagaataag ggctgaaacc atagctgccg aagatgtttt acatgatgaa
     2581 ggtgttttga gcatgtattc gtctgatagc caagcgatgg gcagaatcgg tgaagttgta
     2641 attcgtgctt ggcagacggc ggacaaaatg aaaaagatga agggcaaact gaaagacgag
     2701 ataggagata atgataattt cagagcaaaa agatacattg caaaaacgac gataaatccc
     2761 gcaataacac atggtgtagc ggactatctt ggttcgctag aagttggcaa gtatgctgac
     2821 attgtgatgt atccaacggc attcttccca gcaaagccaa agatggtgtt caagggagga
     2881 ttcattgcct ggtcaattat gggagatccc aacgcatcgc ttccaactcc tgaaccagtc
     2941 ttctacagac cgatgttcgg agcactagga aaggcagtaa agaagacttg ctttactttt
     3001 acgtccaagg cagccatgcg actaggagta ccacaaaaac tagggcttga gaaagtggtt
     3061 ttgccagtaa agaattgtag gaatattggt aagaaggata tgatgtggaa cgacaagact
     3121 ccagagataa aaatagatcc ggagacatat gaagtaacgt tggatggaaa aattgcaacc
     3181 gtcgaaccag caaaagaact ctctttagca caaaggtact ttatggctta ggcagtgata
     3241 gatgcggatg cagagtgagt aaaggaatat aggctaaaat tctttctttt ttttggtagt
     3301 atatcatata cctttatttt tgtctacggt ctctacaaaa tatagtgata cctaatgtca
     3361 aatcctaatt acggtgtatg gagacccatg gcagagaacg tagagttcaa cgccttggaa
     3421 tccaattcca atgcagtgat ggccgtggca gataccgtca gaacaacttt gggtcctaag
     3481 ggattggata agcttctgat agaccaagct atgaacaggc acatatcaaa tgatggtgta
     3541 acaattttgc tttctctgag ggcaatacat ccagttgcaa gaatgattgt agagatagca
     3601 gagaggcagg agcaattagt tggtgatgga acgacaacgg ctgtcgtcat ggcagcagag
     3661 atgataaagg agggcaaaag gctcgtaaag gaacttggag ttcacccaac aaaagttgtc
     3721 gaaggaatag aaggtggggt caaagaagct tgtcagttac tcgaaagaga agcaaagaag
     3781 atctctctta atgagattgc gctagaacag gtagtcaaga catctttgtc atcaaagctg
     3841 gacggacgaa agctctcttt acttgtgatt tcggcactga gatctgttgg aaagaatgcg
     3901 ttgtataatg gctcgtacga ctttgataag acgataatgg ttcttcgaag gaccaccatg
     3961 gaagatagag ttgtcaacgg tatagttctc gagagaaaaa ggatggatcc tgagatgcct
     4021 ttagagataa atgaggcaag agttatgatc gcaagattgg atttgaagcc tgttaaagaa
     4081 tcttgggtga aggaaaatag taagtacgaa gaaattctga atatggaaaa tgacagggtc
     4141 gccaagtcaa aggacatagt taactctctg ctagcaaccg gtgcgaacgc tattttgata
     4201 gcatctggtg aggtagatca ggttatagaa aacctacttg tagcaaacaa agtgtttgct
     4261 gtacgcatcg ccatagaaga gatagaatac ttatcccgtt atactggtgc taaacctgtt
     4321 aggatgatgg atgatctaaa aaaatcagat attttaggca gagcagatca tatctacgaa
     4381 gatgaggata atggagtaat ttacatagat aatgggttac gcggaaatat gactacaatg
     4441 atagtttctg gtacgaccaa ggaaacatct cttgagcggt ggagggctgt catagatggg
     4501 gtaaatgctg cagaagcagc tctgaataag ggtgttgttg ctggcggagg agctgcagaa
     4561 ttgcatgtta ttgagaaggt aaaaaaccta agattgaagg gcctggaaca ggttggtctt
     4621 gatgttgtca cgtcagccct tgagagcatc atgcgtcaaa tacttacaaa tgctggctac
     4681 aatggacttg aaaaagttat ggcggcaaaa gcttctccag atacgtttgg tatagatatc
     4741 gattcaggtg atccagtaga catgtggaag atgggtgtgc ttgatccatt gctggttaag
     4801 accatggctc ttgaggctgc tggagagatt gctaaatctg ttttgcgaat tgatagaaac
     4861 ctagcagcag aggatcttaa ccaacaggct ctatcagaga caaggagatg atttgagaaa
     4921 acgttgattc gacactgtgt tgaagaatct aacgttgatg aaaacttggc cgtaaccgat
     4981 ccttcaaagg taagacatgt ggtaattttg gccggcagaa tagaatcaat gtcaggcttg
     5041 attgatcctg catcacacct taaccttgat tatccagacc ataaggttac aacatgtgtt
     5101 attgctgaaa agtttgagat taacgccaaa gtaaaaattg gtgatcaggg atttgttgtt
     5161 gcgagcgttg gtagatcaac tcttggacat tatggtcatg ttgactacac acaaagattg
     5221 tttgatatga ttgaagcagt aaagaagagc catgaatcga ggaagacgaa agaaaaggag
     5281 aaaatacaat agaaattgat cacagtggat tcagtacttg gaaatatcaa cagagataag
     5341 aaactcaagg aaaaatatga tgaaatgact gcgagaaagg tttgtgagac aattaaaatt
     5401 agcagattag aatctcaacg agtaagaatg agaaaacttt ctgacaaggg gacagatgtc
     5461 gttctgacca ttccaccagg aacttggctt aaaaatggcg atgttataat tataacagaa
     5521 aacaaaatgg ttgttgttgg gatagaaccg gaggacgtga ttatgataga gattagagat
     5581 aacatgcacg aagacgatag tgtcgaagta cctgttaggg tcggtcatac tataggtaat
     5641 ctgcatcgac caataaagtt agagggaaac aaaatatact ttccaattca agcagatacc
     5701 gaactggaga tgttcaagaa gctcttcgaa ccaattaatg aacatctgga tatcaaaaag
     5761 acaacgatgg tgttcgaatc ggaagctgga acagaaatac atgagcattc ataaagcaag
     5821 catagaggaa ataagcatgt tgcagctctc tgattctttt tttccaagtg gtatgtatac
     5881 aacatccagt ggtttggagg ctttatacta cagtaatagg aagattacag atgcggatca
     5941 attgcgcgat ctgataaaag tgtatctgga acatcagata ggcccagctg attgtagtgc
     6001 tcttgggaac tcgtatgaac atatacaaag aaaagatttg cagaaactag tagaagtgga
     6061 tactataata ttctcaatga agcttattga ggaagtaaga aatgcttcga cccgatctgg
     6121 aacgcaggta ctaagatgtg tgagctcatt cagaactgat aacagcataa tgaatggata
     6181 tcaagaagca ataaaggata agagagcttc cggagtttat cctgtagcat tggcggtatg
     6241 tagcaatgcc cttggcattt caaagtataa ggctggtctt atgatgcttt atggtttcag
     6301 cgtaagcatg gtgggggcgg cactaagact gggagtgcta cagcattttg atggacaaag
     6361 aatcattcat gagttaaaac ctagtatatc agagactgta gaaaagaata ttgacagacc
     6421 attaactagc atgtggcagt ttgctccgga tatagatata ctccaaatag cacacgaaag
     6481 aatgtcgtca aaaatgttta ttacttgatt gggctctaag agaatactct tttatgagct
     6541 ctaagagaat tcctagatta ggtattggtg ggcctgttgg ttcaggaaag actatgttga
     6601 tagagcaggt ggttcctaga ttatcatcca gaggttacaa agccgggata gtatcaaatg
     6661 atgtggtctc aagggaagat gctgagcgga tgagacgaaa tcttgctacg gaaaggggtt
     6721 tgatgtctga ggatttggta gtagggcttg caactggagg atgtcctcat acagccgtac
     6781 gcgaagatcc gtcaatgaat atctcggttg tagaggagat ggaatcaaag catagggatc
     6841 tagatttaat cctaattgag agtgggggcg acaatattac caccactttt agccctgcgc
     6901 tagccgatta ctttatttat atcatagatg tctcgggtgg tgacaaatat ccaagaaaaa
     6961 gaggtctcgg gatagagagc tgtgatctct tagtcataaa taagatcgat attgcgcctt
     7021 acgttggcgc ggacctcagt gtgatggaaa gagatgccaa gattgtccga ggcagtaaac
     7081 cctacgtgtt tgtgaattgc aaagacggcc agggaactga ggaagtaaca gagcacataa
     7141 taagagatgt tcttttcgag gcccctccaa acgcgattat aaagagatag gataggatac
     7201 gtagatagta ttaatgagta gcctagaatt ttacacccct gatgatattc cgccggaggt
     7261 ccaggcatac ggatcagagc tgaaacagct gcaagtgggg agagcaggca agatcggtgc
     7321 tctgacatta ttattagagc aagatcaaga aagaggaaag actgttgtca aagaacagta
     7381 ttctaagatt ccacttttca ctcaaagagc actttatttg gaagagtcac tgccttctat
     7441 ggcttatatc tacataatgt ctccttctgg aggaatactg cagggtgaca ggtacaggat
     7501 ggatatagta ctgaaaaata aggcttgtgc tcatataact acacagggag caacgagggt
     7561 ataccgaatg gagagaaatt atgccacgca gatagtaaat gttgttgttg acgaaggttg
     7621 ctaccttgaa tttgttcccg atcaaataat tcctttccgt gactcacggt tctaccagac
     7681 agttaatttg aaggttcatg aaaatggaac aatgatctat tccgaaatga tagttcctgg
//
LOCUS       DAOR01000134            2985 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_108454, whole genome shotgun sequence.
ACCESSION   DAOR01000134 DAOR01000000
VERSION     DAOR01000134.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_108454"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1462..1652
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          985 a          445 c          553 g          811 t
ORIGIN      
        1 aaaaaaagca tcaaaaatat aacaaaacac tttttaaaga agaaggaatc tagaaagtat
       61 gtcagaagaa caacaagaag ttcatatcgt acttgtatcc aatgatccta atagagcata
      121 tccagcacta atacttgcat taggcactat aacgatggga gcgaagtgca agctatattg
      181 tacaatgagc gggttggatg tcgtgaagag gggaggtgcg tctaaattaa cgatgccagg
      241 tgcgcctcca ttagagaagt atcttaagga tgccatcgat gcaggagtag aagtcactgc
      301 ttgtggacca tcaaaagaaa tgcttgcgca aatgggcatt acaaaggata atttggaaca
      361 aggtgtccag ttcgaagatg ttgtaggatt tctaaaagct gcactaccag cagcaaagaa
      421 gggtggtatg gtattattta tataaagtaa ccagacacat tatatgaata tacagaaatg
      481 ctaaaggatt taaaaatagt aatgaacaga tcttctattg atctattaaa agctaatgta
      541 gtaaagttcg ttgatacgag aggtatggca tgtccttatc cgtcatttga aacggtaaag
      601 gctatgaatt ccatcaatcc aggaggtgtt cttgagatac taacagatag tgatgaatca
      661 gcttttaaat ccataccgag tgtctgcgaa aagcgtaagt gggaattctc agtgtcagaa
      721 gaatcaaagg atgtttggcg tattcgtatt aagaagtaaa aaaatctact agcagcaata
      781 aatctatttt tgaaaccagt gaagccggcc aaccaataat ctagttgtaa ctgaataatc
      841 tgtttatttc cttgattagg tgtagtactt tggcgccagt aacctaggtt cttattttgg
      901 ttctaatctg gctaactatg aaatcaagca acaaaagaac gtaagtgctg ggatgactaa
      961 tatgtttcat aactaaaacg actacgattg catggaataa ataaatctga tcttatcgta
     1021 atgactatga agggattgcc tacgtctacc ctagcaatct gataacaccg ataataatgc
     1081 caagaatcaa acaggtgaat ccaaatgcca gaagtttctt cctcatttac tgtccatctg
     1141 ttgaattgat ttgaggcaat gtgcccgtgt aatttgcaca atgtaggtta tatgtaaaga
     1201 cccgctcatt aaattgtccg cttccaataa tgccaactga ttgtactacg atattctttg
     1261 aagttgtact ccaaaattat ttttggtaag agtcattatg aaggcggcaa gaaatactgt
     1321 agaagatgtg aagtttatta tcaccacgat ggtttgttct gttcttgttg tggcatggca
     1381 ttacgcatgt cacctactag taaaagagat aaggaaaggc tgaggcaatt acaacttaga
     1441 agagaagaac aagatagaat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaataatc aggattataa agggaacaaa
     1681 gtaactacac ttagtagtaa cacctactga cactgaatta ttgctcacgc ttgttcaaga
     1741 ccatgaatag tttgtttttt tgcttcagaa tatataagaa tgggtaaatg ataataaata
     1801 gcatattatg ctataagtat acaatatgga tataactaat gcagaaccag agtctacggt
     1861 agatacatcg atcgcgtcat ccgctcaaat tcgatataaa aacatattag tacctcatga
     1921 tgggtctgaa atggccgata atgctttaaa acatgctatt tacttatcaa atatatctga
     1981 agccgaaatt attatcttaa acgtagtcga acatcttgat agtgttgact ctagtgcggt
     2041 gttggcgact agcagagaag gaaatgaagc aaaaaaagaa ccaacaagtg attatgaaat
     2101 tacattagaa ggacaagtaa aaaacatggt agaagagaaa ataagattat gcaaacaagc
     2161 tggacttaaa agtcaagtat cctataaaat acaaaccggt aagccagtcg aagagataat
     2221 caaggtttca gaagagatga atgtagattt aatagtaatg gcaagcagta gaagttcttc
     2281 gttggtaaga agaattcttg gaagcacagt cagaaaggtt attgatagtg ttaaaaaccc
     2341 agtactcgtt gtacacgaat aataagcaat caggtagaca gataatataa cgccaaaaaa
     2401 cagaaatgag ataggaaaag cattaaattg aaggtttagg gatttttgat ggatcaaatg
     2461 ggctgccctg tctgtacttc cgctaagggt ttgaaagatc gacgagaatt agtcgaacat
     2521 atgaaaaccc acaagaacaa tccagattta atgatagcta actattcaga tcctacttta
     2581 ttatattgga atagatggta ttagcagctg catctctact gctgactgct attccgtgtg
     2641 aactagattt tattatcgta ttattatata tcttgttatc agaagagtca gatcctacgt
     2701 tgataccatt tgcgctatct gaaactatgt ttttgtatat ttgattatgc gactgtgata
     2761 caaagatacc attatcctca ttataaacaa tattgtttct tggtatagag ttcgtcatat
     2821 tccgcctaaa cattattcct gaagccgcat tatcatgaac tttgttattc tcaattagta
     2881 tattgtaaca gtcaagtgaa cagattatcc tatccctcca tttccgaaga ctgtattatt
     2941 tctaattatc aatcatgtgt ccctgtatga ggatcaagac cataa
//
LOCUS       DAOR01000135            3336 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_108455, whole genome shotgun sequence.
ACCESSION   DAOR01000135 DAOR01000000
VERSION     DAOR01000135.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_108455"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2251..2385
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2722..2916
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          961 a          536 c          544 g          965 t
ORIGIN      
        1 gattataatt gatgcaacca ctagaggaac cattactcga ttgagcagga gacgaaggcg
       61 aaattgtatt tccctccttt gaagatggcg catgaaggga actagaagga ggggttttat
      121 ccttgacagc ttttgcttca tgtagagaaa atggaagcat gctgagtatt atatactgag
      181 aaatgacaac tgtaagtgta acaacaataa agattttcgt agttatccag aaatggtagt
      241 tcaagttgtt ttaaggaaat tttatggtgg aatatatgtt tttgactcac tcaccttgag
      301 gatttcaatg tgaagagatt tggccgggtc tctaaataac tcacataaca gctttgcagt
      361 catttgtcat gcatactttg ttgaaacagt aataaattta gggcataatc tttatcgcgt
      421 aaactggatg tagctctgta attcctcccg gaacatctca attagatatc taccaaccat
      481 tacatgctga cctatcgtga attacgtgaa aagtaggttt atcgcacctt ctcttctaat
      541 gcctttatcc gttcttctaa ctgtgccaca aaacacaaaa catacatgtt ctctcgtccc
      601 tatttaatga gtaaagtgga gagtttcttc aaaatattat atttttcttt aacttatctg
      661 ggtctgtaga tatatcatat ttccacgtct tattgacttt atcccaatcc atataaaaat
      721 aggcgttctt taggactaat tcaattaatg tcaatttctg tgtatcaaaa aaatatgcgt
      781 tgtatcaata aattcgccac ttgtccattc aacgggtgca actttacgat ttgagttaag
      841 gtctatcact ttcgcagacg gaatgggagt cggctttatt tgctactttt acgatatatt
      901 gcattgtcct tcacatcacg gataagcttc taccataggt gtgttgcaga aatctggaac
      961 cacagatatt gatatagatg aattcaatct tgttgaatcg gattccagaa aagataaaga
     1021 ggcgagaatt aattatgcta gcgagcgcga aacaaaaaca aagatcttgg aacgctctat
     1081 ttacttgaca ggatatgata atttaaactt acaacgttca aaggaatatc acatacttgg
     1141 ctggaaagac agacctagta aattatccac aggagattat gtatttgtgt ttaactctac
     1201 tggagacaag atagattctt gctttgaagt taaatcctta tcaagcaaca aaaaccctat
     1261 ttggcatgag gaaagtagct ccagagcagt tgtcattact aactgcattt cttaattcta
     1321 accaagatac aagaatgtca atatcaaacc atcattcgaa caaataaaaa tcacaacaag
     1381 aacaacgata ggaattcaag gtttgtttaa gtcactacta ctttaccatc catccaggga
     1441 tggagtaggc aatcatagtt gtatgtacct gctttttgaa atgttacgct aaatgtactt
     1501 gaacttccag gaagtgggcc cttcggaaac atccagcctg aattaacata cttttcacta
     1561 ccagttattg taaggcttgc atttggcgga taaatttttg tgttatcgtt tgagtcgatt
     1621 actgctggac taaacacacg ttgattcgcg ggtagaaaac catttatcat cgttttgtta
     1681 tcaaatacaa acgttactgt gtgaggttcc gctggggcta aaggtttaac ataccagact
     1741 acgctctctc caaccttaat attcacattt tgtggtgtat atccaaatag tggataggtt
     1801 ggattacctc ctccaacctg cactccgaca gcggggcttg ttgttggtac tgctgctttt
     1861 gcattgtaac taaataatat ggaaattgct accgcgctca caataaaccc taacacaaaa
     1921 gcgatgcttg cattttattc ttgtgcgaca ctccaatcat ttaagaatat caatctacat
     1981 gaatgatata accatttcta aatctggcgt cttattaatg gcgtatggaa gcatttttac
     2041 gcttgtacct tgatcgtaca tgatattgta gattgtaata tattcgtaaa agaggattat
     2101 cttgaaagta tggcgcaaac tagaacctaa aattagagag atgcgagacg gtaatgggcc
     2161 atctgattac atgaaaaact ttaagcaact gaaaactaaa gcggaagaat atgcaaagag
     2221 aaaactaaag cggaagaata tgcaaagaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagaa tatgcaaaga
     2401 gacatcatat gggagatttg gaaaaactcc agaaacagag gcattagtgg ctctatttct
     2461 accactaggt aaggcgatta actcgtttct tagccttcct accaacaaag taaagtcaaa
     2521 ataatttatg catcattccc agtttaaaat tggtaaccga tttatagact ggtaacatac
     2581 caaaagtaag tgtctatttt attggttcaa aaaataggat aaaaagagta ctaatttaag
     2641 agtcccttcg agcgctactc gttgataaaa tactctttca cttggtgtta gggtatgatt
     2701 atcattatta ttattattat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatta ttattattat ttttattatt
     2941 gcgcatgatt taggctatcc ttatcctcat ctatcttggt aatcccaaga ctattatcat
     3001 tttcattacc gatatcggag ccgtcaccaa agtttttact catataatta cggatttgtt
     3061 gatcttcagt taatgccaga ggtttataat tacctgtatc aattcccaac gagttagcag
     3121 agctttatag ttaatgcttt atcaatatac ttaatggctt cataatactt tcctaaattg
     3181 gcaggggcta cgcctattcc atttaatgag tcaacatcat tgggatctat ggctaaaacc
     3241 ttatcaaaat aggcaataga ttcattattt ctccctaatg actctaggat tatgcctttg
     3301 ttagccagtg cagtagtgta gttaggtctt atagat
//
LOCUS       DAOR01000136            9630 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_49492, whole genome shotgun sequence.
ACCESSION   DAOR01000136 DAOR01000000
VERSION     DAOR01000136.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_49492"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3103 a         1751 c         1880 g         2896 t
ORIGIN      
        1 tatggcgtta tgcgtatttt actcaaaact gtgatagatg actattatgc aaatctttta
       61 agattgtata gggccgcgaa tcccaagggt tattcaaatc taaaaaagac atacacaagg
      121 aaggatagca ggatactcga atctggtttt tctgattcgc aggtttgggg tgctttagat
      181 aagtgctgga cagggtacaa aattgcaaag tcggaattgg acgcaaaaaa agatgaaata
      241 ctatgctaag ggtattcgga aatttcaaag ggaattggag atatcagtag cagattttcc
      301 acaactaggt ttaataggtg aattgaggaa tgacgaagaa cgagattcaa acaatgattg
      361 gacatgccgt aattctagag atgtagagaa agaaacagaa gactatgaat tacaaaaagt
      421 agctgaagat ccttttagaa tcgaacgtga acccgatcta agtaaagaac aagtggaata
      481 cttcagacgg atacgtcagg aatggatgga tcctggatct tctggataga aaatggaagg
      541 taaggaactc ggatgactac ttcagaaaag tagtatcaga aaaccaagat ccatagacaa
      601 aacttttcat ctatcacaaa tgaaatagcc tcaagatatt ttagttggga cgagtaatat
      661 aataaataac aagagagaag aaatgaaagg acgatacagg taccgaaaca agaaaaacag
      721 agatgataaa ttccctagcc atagtagtca atctacaatc ctgatgtcgg gttatactgt
      781 caatcaaaca tggaagggcc taaagaaggc atggcttggc tacactattg caaagaataa
      841 aggagaagac gacagaatgg tctattatgc atcggtgatt cagaaattac gaaaggaatt
      901 agatctaccg cctgcgagtt ttccacatct agatatcgat aaagacataa caaaggaaaa
      961 gtccaataag aataagaata agggtttaga taaggagaat aaccattagt ctgatctttt
     1021 acagaagaaa ctccaatacg attcccaatt agttaatgat tcatgcacat cctccaaaag
     1081 ctgcattatt gatatatgtt gaaggataat aaaggcccgt atttctcttc caaaaattta
     1141 tcgatttcca ccttcgcatt acacacctgc gggaacgcgt ctttaaaatc ataaggtttt
     1201 gttgcaatga ctcgtgtcat ttcttccaac acaaatccaa tcataatagc tagtactccg
     1261 tatctggttc gaaggtcttt cttatcgtta taaagatatt gcacaaattc atttgagtca
     1321 tctatgatta cggtatttgg tgcttcatac aacatatatg cgccaccacc agcaactttg
     1381 tcgctgtgaa ctaactttat tggtagaata gaatctaaat tccatttcta agaatataat
     1441 ttaaggcttc aagacattta agatcacatt ctgcaattgt tttcaactct ttgagacgat
     1501 gtcctagaca ttactctgag aagcttataa cttaattaca ataataacat gttgcaattt
     1561 tcatgtttga taactcctcc agcctttgac gcatgacaat taaattgaca ttgttctgtg
     1621 tttgagacac gaggtggtcg gttggagagc tatacaatac cactgtgaag ctttttgatg
     1681 tcatctttag ggtatgattt tatcaatttc gactttccac acgtagaaaa tcaacgcggt
     1741 gaagaaatat acacttgttt gacgcacccc tcaacgttga ctgtcgtaat tggggcaaag
     1801 tgttcagatg gtattacatt gattgctgac agaaaattga cacgaagaaa tggtgaagtg
     1861 tgttttaacg ataaaatttt tggagacata gaacacatcc ttatcggata tacaggcgat
     1921 gcagatttgt tcgatatttt caggagatac accgtgggag atataatgat tgaaagagat
     1981 aatttgaaac gttatctact agacaatttg cttttgaaag tatccaagtc aattaaacga
     2041 tttaacgcgt tacgctgcac tccatttaaa gtgttgatgg ccagtcacaa agagaaccct
     2101 ccagaattgt attatattca tgaggatggc aattggaaca aggtagaaca ataccaggcc
     2161 attggaagcg gttcaacagt ggccgaaatg gtatgcggcg gattggaaca taacaatatt
     2221 acaatgaggg atttcgcaag acgtgcttac ttggccattg attatatgca tcagtattgt
     2281 ccaggactcg gagtgggagt tgagcctcaa ggcgctccag atattaaata cttacattat
     2341 aacgaagata aggacaaacc agcttcagaa caggacaaga aggagtacaa aaaatactca
     2401 caggaaagat tacaacgatt caaacaaagc ttcgatacaa ttcttaagga atagtatttc
     2461 ggttccggta atactattcc ggcctgtaag ttgattttgg acgaatcggc tatcttcgta
     2521 ggctaatttt aataaactaa tacttaggcc ctcggtaaat aatatttttt gcagtatctc
     2581 tagatttaac cagtaatcac taaggctagg tttctcgttc tcgcacaatt ggtgattctg
     2641 ccaagaaaaa taagataaga ttatggttta aacagtacaa tcctgggtct gggaatgaaa
     2701 cccacaaatt cattcatttt ctacacacct acattttcat tgattgtgat cccaaaagct
     2761 ctgagggtag taccgcacaa gccatggtga cttcatcttc tataacattg ttctcgtttt
     2821 cacgaaggaa aataggcaaa aatgagcgta aatttgccaa gtcaccacgc cttgtatttt
     2881 gctctacaaa tataggcaat cctcattcct ctatagttga tggtttgagt accatggtct
     2941 tgggtgataa aagcaagatt gtaaaagtat tatcatggta tgataacgag tggggttttg
     3001 cttgcagatt gattgaccta ataaaattcg ttgctaatat gatgcactca taatactttc
     3061 atattttcag catgagatca aggtaacaca atggaatcat accaacaaaa tagatatcaa
     3121 ggtggtttcg gtcaattatt aatattgagt atggcactga aaattcataa gcggatcttg
     3181 acaacgattc ttattgcttt atggtattcc cgtgcgataa tattatttct gcgattcaat
     3241 ttaatacttc aattccgggc atctcatttc ctgcaaggaa cctgagagta gcaccaccac
     3301 cagttgacac atggctaacc tcaccttctg aaactccagc tcttttcatt gcttctaatg
     3361 tatctccacc tcctattagc gttttggaac ctctccaata agccagtgcc atactttttg
     3421 caatatttac ggtaccattg gagaaaggcc ctatttcaaa tagccccata ggtccattcc
     3481 aaaatattgt tcctgctctc tttgcactaa tttggctgga atagagctgt gaagtttcga
     3541 ttccaatatc aaaaccaatc ataccatctg gaatattgcc tttgatgaga ctagcagaga
     3601 cagaagaact agcatcttgt ggcgaagagc atactatgtg atctgttggt agagttatca
     3661 tgtcaccata ttttgaaagt gctttagtta cccaagaaag atgttcctta tcaattggtg
     3721 aattgcctgt tttgaaacct tttgctttaa gaaatgtata tgcagtagcg ccacctacaa
     3781 gaagattatc tgcttttggc aacaaatttt ccagagcgcc aattttatct gttattttca
     3841 ctcctcctat aacaagagtg aagggttttg gtggattgtc ctttatcatt gaaagatatt
     3901 cgatttcttt tttgagattg agcccggcca ctttgatatc aaatagttct gctgtaccat
     3961 aagttgagga atgttttcta tgagatgtac taaattcatc atttacataa acgtctgcta
     4021 atgaagcaag ctttttagca aaatctggct cattgttctc ttcttcctta tgaaaccgaa
     4081 gattttctaa caatagaata tcacccatat tcatttcatc tatctctttt taaacatgtg
     4141 ctccaacaca atcagcagca aatctaactt tatttgctgg catgatttct gataatcttc
     4201 ttgctactgg tgctaatgag taatgactat cctgatctga agttggtcta cctaaatgag
     4261 acatcaatat cagcttggct cctcgcttgg taagatactc tatagtcgga atggtcattc
     4321 taattctgta gtcttcacca attataccat tgttattgat acttacattg aaatcaactc
     4381 tcgtcaatac tttcttgcca taatattcta gatcgtccag atctaagata gcttttggca
     4441 tttttctgtc tgtgttctgt tcaattactt cagacatatt cctcttactt ttggtttccg
     4501 agacagtcat atctagattt aagcattttc cttaaagaaa cttcataatt atcatctacg
     4561 tttgcagtgt catgcacaag attcaaacat ataatatatg gctgagattg caatcataat
     4621 atgcgaacga caatatctgc gattaaagca gacataggtg gcattggagg acacaccagg
     4681 ccaagcgatg agcttgttga agcagtaaag aagtacgtta cccaaagatt aaaagaagaa
     4741 gcgcttttaa tcgactatta cattggctat acaggtgatg atatacacat cataatgaca
     4801 catgcacttg gtgtagaaaa tgaaaagatt cacaaacttg cgtgggatgc atttaaagaa
     4861 ggaaccaagt tagctaaaga acaaggacta tacggcgctg gccaagatct tttaaaggat
     4921 gcgttttcag gtaacgtcaa gggcatgggc ccaggtgtcg cagaaattga aatggaagag
     4981 cgtcccagtg aagtcttttg tatacttgca gcggataaga cagagcctgg tgcattcaat
     5041 ttcccgttct ggaggatgtt tgtagatgca agaagcaatt ctggattaat tattaatgaa
     5101 aaacttgcat ctggagtaac atttagaata ttagatgtca tgagcggtaa agttgcagaa
     5161 ctaaaattgt gggaagacaa acctgaacta gaggcatctt tgatgtatcc gggaagatat
     5221 gttgtgcatt ctgttttgtc ttctgatgac gaaagagaac caatcgtgtc ttcctctact
     5281 gatagattac ataatattgc tggaacttat gttggtaagg atgatcctat tgcaattata
     5341 agaacacaga agaattttcc tgcaactgaa gaagcaggta gtgcttttaa taaccctcat
     5401 tacgtatctg gaaatactcg tggaagccac catttgccgc ttatgccagt taaattaaat
     5461 tctgcagcta gtatcaacta cgccattcct attgtctctt gtttggtctt cagtatgcat
     5521 aatggtaaac taacaggacc acatgatggg tttggtactg cagactggga tttgcaaaga
     5581 ttatgggcgt ccgagagagc aatgattatg cgcaaccaag gattcattca tcctgctact
     5641 ctggtaccag aggaactgga gtataacaaa ggatacgaag ctagaatgga caagctgaat
     5701 aggaaattta aagacgtgga tagaaaagct aacgcaacag gcgttaactg actagctagc
     5761 tgactactag aggaagatca ggctttaaga aactattacc tggaagtgcc gcatcaagag
     5821 ttgtcactta tgcaaattgt cctgttctgg tagtgaagtg aaatctatag atgaaataca
     5881 aaaaatgttg ttgtcgctat atttaaaaat aaacaaggat tatgtattct tatgactatg
     5941 agcgtcgccg atataatgaa gaaaaaactt gaaaccatcg aagaagtggc ttctattcaa
     6001 caaacagcga agaagatgaa agaaaagaat gtcagttcgt tggttgtagt tgacgctaat
     6061 gatagaccgc aaggtttagt tactgaacgt gatttggcca gaaaggtttg cattaataat
     6121 gtacctgcta acgcagtgac aaataaagag ataatgtcgt ctcctcttat aactattagt
     6181 actgactcat ctccatcagt ggcagcagac atgatgttac aacataatgt aagacattta
     6241 cttgttgttg ataagaataa cctaaccaat acgagacctg tcggcatggt tactccactt
     6301 gactttacaa gatatcagga attcacaaat gatagtgcgg ataaagacgc tattgaaaag
     6361 atattggaat attatatatg aaaatccatt catgtgtgag gatatgaaaa tttaactgtt
     6421 cttgataatc caaatctttc tatggatgaa atctgacttg ttattggata tacaatatct
     6481 tttttgctag cttagcctac ctcacgatta taactggaca tttagaataa tgactaatct
     6541 tgtatgaaac acttcccaac agaaattcct tatgggcctt atgtcctcta ctacctatga
     6601 ttatcgtatc gatgttttct ttatccgcta tacgtaaaat ctctttagtt acatcatctt
     6661 gccggagctt ctagtatgtc tacattggtc agacctgatt ttatagcttt agatcgatat
     6721 ttttttgcaa cattgagata ttactctttt aattctgtaa caagagaatc atgctctttc
     6781 catctatcag ctagctgcgt atgcatgtac ataatcgaaa gtagctagag ttatgattat
     6841 tacttctgaa ttttcgtgaa tgattgtgat tgcaaaaaga tctgatcacg gagtgtcaga
     6901 gacttcatct attcaaattg caactttgtc aatattttag ccatttcctg ataattgcgg
     6961 cgaatcatat aaaacagggg ctataattaa aacacttaaa aaaactttgg tccgtatata
     7021 tgggtagtaa gtaaaaatgg ccatctcgca gtgtgttaca ttgcagaagg acgtttcact
     7081 cagagggtaa aattcctaaa attgcgttat cagaagagta ctctcgattg gagtgctgtg
     7141 attgtgagaa gaaaagggaa agtcaagagc atctctcaga cgtattatct catgccaagt
     7201 gtcctagatg ttatactagg gataaggagc tactgaatat gctggaaata attacattct
     7261 cagatgcaaa agttgtgggt gggaatggac tgaaggctgg catccaagta tgacaaaaaa
     7321 aggtaaacca tcgcctaagt agtgtgaaat gaccataaga aaaggtaccg cagatcctca
     7381 aataggagga gttagaatat atcgacaatg gacattcgaa tatgttgcgt tgttatctca
     7441 atatcggttt taacaataat cccagtcatg atcatggtgt atgcacaggt tggaggaaat
     7501 ggggttctca tacatatacg cactgataac gaaacatcct ggaaaggcga ctacagatca
     7561 ggacctggta tccaaccgat cttgtacacg taatacaagt aatactgaaa ggtgaaagct
     7621 accggattga taagtataac aaactagcaa aaaaatatca tacgaaagat ttggttactc
     7681 acgaaatatt tgaaggttta cttgtaaatg aggttagaga gaggaagctt gaagccgtgg
     7741 taatagtaac ttcctgaatt ttttgtcttg cgagctttat agagtctgca gatatcctta
     7801 caaaactttc tcaatgttcc tattatcctt tttgttacaa cagataactt tactttgagc
     7861 accgatagtc tatgccttgg ttcttattat ttcaactagc ttcctatcac tagctattgg
     7921 aaatcagtcg caactaatag tgtcgatggg actagttctg gtatttttgg cctggattta
     7981 agggagggta caaaagatat agtgcccgca atgaatccat ctatcaagtt ccatcattaa
     8041 tcaaaaccag tgatttttct caaatgttta catatagatg ctaaccaaag acaatatact
     8101 aatgataaac cccttcaaaa gatgcctaca tatgaaaatt aataagaatg tgatattgac
     8161 ggtcgttctg ataaccattg taatggcagc aggttctaat caaatatttc taaaaacatc
     8221 agcagtagtc ctacaggagc aacaaccatt gaagatcaca aacaatacta gcaacgctac
     8281 taaggcaact acccctacaa acattcgtcc tagcattcaa actgatttca atccaaatgt
     8341 cacatctcca agaatatcta aaactgcatt tgttgatcct cttgcaataa ttatcggtga
     8401 ctgtgagatt ggaaagatag tattggttgc accgtttgca gtatgtagag gtgatgaggg
     8461 tactcctatt catattggag actattccaa tgtgcaggat ggtgtaattt tacatgctct
     8521 tgaaacaaca gatcatggta agaatattga tgatagaaga tattcagccc aaggatctct
     8581 tttgaaggcg aatgatactg cgtttaagaa tggttttgct gtatatattg gtgataaagt
     8641 cagtttagct catggaacaa tggttcatgg gcctgcgtat gttggaaatg atacatttgt
     8701 ggggatgaag agcttcatct ttaatgcgaa agttggcaag cgagttgcta taggagtatc
     8761 aagcacggta accgacggtg tcacaatcgc tgacaacaaa tttgtaccac caggaagcat
     8821 tataactaca caagcgcaag cagatgcatt gccagctaga attggaagtc catatgagaa
     8881 gatcaatagt ttcgtactcc atgtgaacca agaacttgca aacccctccc cgcctgattt
     8941 tttaattcta atgtaatatc ccttggtata gtaccgctcc tgctcctctt gatatgttcc
     9001 tttaggtcta acaaattctc cgctagatga taattttgga gtcttctctt cttcgcgctg
     9061 ttccttatac caatcccgta acaaccccac cagatcagca cccgacccta tccaagtcaa
     9121 gaaacccact acaaccgctg ctcccaatag aacggttatt tgtttttgta gagcattgga
     9181 aaaataaaat cctcctgcaa atccgattgc aatcaaaagt ccatagaata atccataacc
     9241 ccaccactgc atactctcta cgcatagaat ctccccttat tatcgaaacg gccatgcaac
     9301 tttgacattc catttttgac atgcgttatc ttgacaatac ccaatttttt attaacttct
     9361 tgtggtgtcg gaaaatctct cgtttctgga tatctatcct ttaacgcgtt acaaagttgg
     9421 cagccaccta gttttgggtg acatttagag gtctgccttg taggaaaaat tcttgtccaa
     9481 agggtgccta tatggtgccg cataaagttt cattcttttt aaaattttgc agaagaatac
     9541 gaaggtgaat caggtgttaa tgctaagcct tataaagaag agaaaaacaa ccgcagagca
     9601 gtttattatg gcgttatgcg tattttactc
//
LOCUS       DAOR01000137            8483 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_16634, whole genome shotgun sequence.
ACCESSION   DAOR01000137 DAOR01000000
VERSION     DAOR01000137.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8483)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_16634"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2565 a         1699 c         1551 g         2668 t
ORIGIN      
        1 tctagcaaga tctggtagtg caccgttgat gtttttaaac tgtaacggct acgtgaaaca
       61 accgcagact gattgcagta catattcagc caatggtaag ctcacttatg caaatcgatt
      121 tgatatcaga agtgatagct ttagacatgt agacagtagg cacaattata gccctgctca
      181 ggagtatacg caattcgttc aacgtatgga tagtcaaatt ccatacaaca caatcggaaa
      241 gataaatgca attcctataa ttacatatca caatcttaca aacagcatac aagattataa
      301 caagatggct tccactataa ctgtggattt atttgctcag gaaatgaaat atctacatga
      361 caatggattt agggtattat tattaaatca acttggatat gatccaaaca gtaacgtttt
      421 tgatataaag aatgttccat tgtctactta tggaactaca actaacgctg ctactctggt
      481 cagagctcca tcctagtcta aattcagtat taaataaaat tctagaatgg cattacgaca
      541 tgtccgagac ctgtcaaatt attcttggtg gctttataac gctagcttac atgaatcact
      601 ggaacattgt gtttcagact gtgtgtgagc atgttatttg aactatcaag actacgcaga
      661 aaatctgaga ttgaaataag ctagttgtta taacatagta gctaactatc tcgaagttgg
      721 cggccactat acaattccga ttgcatccac agggtctcaa atatggaaac atgagttcaa
      781 acggtagctt cgctattaga atacgttgcc aatcctgttg tttcgtcagg gttgacttct
      841 atctccaagg acaattttct gtcaactatc aatgttgtaa cttttgtttg caaccgcgtt
      901 tgaaggtgca cgatttcaat cttgctatag ttttcatcta ctctcatcct ctgtagtaat
      961 tcctccattt ttcgttttat catctaattg tatcaatatt ctaattcgtg cttgtcgcta
     1021 ttgcttataa tagcatgaga taggtgttct ttatcatgag aaattacgtg aatcagacac
     1081 tccttcttgt cagctattcc aacattactt ctaattccgt ctaaatgtcg aaattctata
     1141 aactccatta gcttcttaca ataaagtata ttttcatgcg tgactacaat gacgcctctt
     1201 atcttaacac ccctattcgt gaggaaaatt aatccgttga ataactggtc gtctaataca
     1261 tgcatagcta cttcagtatg gtctaaacat gcatcaagtc tttcttttat ctgagaaaaa
     1321 accttcactg ttactctttg tatgttttta ttaccataca acacctctgt cctttcagta
     1381 accgaacctt gggaatccgt tatactatgc acccgtactg gacaaacaat aaggcaatta
     1441 ttgtattttt atatttgcgc ctgcgtctgt ttatttagta atagaaaggg gtctgccgaa
     1501 aacaggaagg ttgttttaaa tcattattcc aaccctgaat catttctaca attaattagc
     1561 cagtcgtttt tactactttg atgccttgtt caattgtctt ctgaaaagcg caccagcgat
     1621 aggattgata gcatagggca gagtatgctt aaaccacact cctgctctta cataaaaagg
     1681 aactattatt tcaagacgcg aggaattagc tgcagcaaga attgcttttg atacagttct
     1741 tgcttcgagc ataaagccag tataatttgg cttatgattc tcaaaagatt gattgttgaa
     1801 gaaatttgtt ttcacgccaa tagggcttac tactgttatg cgtacaccag ttccatatag
     1861 ctcgtgatac agggactcag agaatcctaa cattgcatat tttgaagcac aatatccagc
     1921 cattccagca actccgaaac ttgcggctaa tgatgccaca tttacaatat gtcctgaatg
     1981 tcttgagagc atagaatgca agaatacctt ggtacaatag accattccaa aataatttgt
     2041 ccccatgact gcctctattt gctcaatact ctgattttcc actttaccaa attctccaaa
     2101 acctgcatta ttgatcaata aatcaatata cccaaatctt tcgagtattt gtgttcccat
     2161 tcttagaaca tcttcctttt ttgacatatc acaagtatgt gctacaattt ctgctccttt
     2221 ggatttcgtg acaacttgta acgttttctt tagttcgagt aattttgatt ctgagcgagc
     2281 tataagtatt attggacccg cgcctttatt tacaaaatca agagcagcct gccttccaat
     2341 tcccgaagaa gctccggtta ttactacaat ctgtccttca aactttctct tctttctgtg
     2401 gccttcgtcc tctttcccct tccctctcaa tcgatctcgg atattattat tgcagaacca
     2461 gcaaatcatt gtttgcaaaa cgagatcgtc aaaaaagagg taaacaatcg atacaccttt
     2521 gttaacagct aattatatcc gtcttttacc tcatttgaat actgctgcag cagtttgtgg
     2581 atttggggtc aatgacaaat ctgcaatatg gagcatcaag atgttccctc atagcaattt
     2641 ttgtgataat cctctgcaac ataaatctct acattaaacc agttttcctg agttccggtg
     2701 aaatgccttg atagatcaga aatgcgttaa gcacatattc gcatcagaaa ctttcaccaa
     2761 ctggcgacgc tgctatggat acattacgtt acgttaacgg gaacaaagtg tttaactgac
     2821 gctctgatta ccttggcaaa cgcgttcaag gaagaggtgt tgaatctacc aacgaaaatt
     2881 ttagtcctca gactggtgat caacttattg aataccagag aaaaagcaac taatccactc
     2941 tttaatagaa gcattgaatg catactcttg caatgaacta cccctgataa aagatataaa
     3001 caagatgcgt gaataaacat ggttgagtgg aaaatgcata aggacggatt agaaaacttc
     3061 tattcaattg gtgacgcttg actacggcta tgcaaatttt tttctattct tctccactat
     3121 ttactcacta aagctagctt tacctgcttg tacatacaag gctttactgt cttgtcggcc
     3181 accctgcaga atgttaggtg ccaggctgct tcaagtccta agagagtttc tacaactcca
     3241 actgccggat agattatata ctcaaacaca ttatctctac catcaacaaa cccgttccat
     3301 taccccgttt taaatcctct cgagtcaccg aattaatgag cattcccgac atactaaata
     3361 atagtagctt cagttaattg ttggtcaact ttatcattag ctagtcgact accaagtaat
     3421 actttccatg ggttattata tcttgcatga aatattattc tcttatacct tcgaggtttg
     3481 agacaacctg ccatagcatt gttctaatat gcgattctat tcttttatct tgactcatgc
     3541 tgtctaatat actaatagcg tttgccgctc taatcgataa actagttttt gggtcgtgag
     3601 ctttcaacag aagaagtaat tcatgtatcc tgccttttga agttctattg cctctacaat
     3661 aattagcact aatgttttcg agggtaccta ttgcagcagc caagctttct tggttagttt
     3721 tgttctgtaa tagttctttt tcatgtttcg ttatcctttt tgttgttgta gttttttcag
     3781 aatatgtcat tgtaacaata cctacatgtc tatcgtatta cttgctatat atcacctatg
     3841 tggtattgtt tgtcctcaaa tcgatatgtt tttaagcgag ttaagttatc gaatcacttc
     3901 cctaaagcat tagggattct atatcttctg attgtctttc cttttgacca taattattat
     3961 gctattttca aagtatgtta caaggggcct cttctttctt ccttttctgg tacagcagat
     4021 ctatccgaca tttctttgtg gtgagataat tgaagctggg gtgggagtat aagatcatca
     4081 gaaaaggatt tgacatgtat actgaaggtc ataatatttg gatgtatggc aacaatggcg
     4141 gcaggaatgg ttcttactat agtagtcctt ctatcacatg caacatgaca cattcgtagt
     4201 atgtaatttc tacaaaaatc cggcgtggtc aaagcgtatg attaggagtc ggtgcgaatc
     4261 tgatgttgct tatctccaca ggaaatggag ccattcccag tatattgagt tagagatccg
     4321 atgttcaatt atcatctggt cattataata aaacagaaat gtccttaatt aattttagat
     4381 aatgcacggt taaacttctc ttctctccta ccagaatcct gagaagcatg cgcgagaaga
     4441 tgttttgttg cgattcttaa cttacctaca aatttcttta tttcttcttc agtaaagcaa
     4501 aactttggac atgctctact cgttccggag aaacacatgt aactttactt aactccctct
     4561 ccaaatgcgt ctcgacacag ccgtatttaa gatttgaata atctctagac tgatacatta
     4621 ggtcttttat tgtagcaatt gacctttcat accagatcat ataagtcagc ttcaagtcat
     4681 caatgattag cataccatgt tttctagaat tgtcagcgta ctactgcttc cgccactaaa
     4741 ctaggaatta ctactacgcc atagggttgg acttgtgtta tttttttcgt ctttgtttgt
     4801 ttttctagtg tcaatcatgt gactaaggct atcgcataat tctaacacgt gcgacccctt
     4861 tcccgtaatt ttataggttt tcttaatctc accatccgct aacaagccat ttctcaatag
     4921 gagtggaaaa tacacctcaa gttgtgaaaa agacagaaaa gcgtcataca ttattcttgt
     4981 tttaaaagcg ccattctcct tactagcaga tcttaacatg agcgtaatta tttccgtttt
     5041 gtctctgtat ttcatttatg ggaggaaaaa tgaagattga ctgtggttgg atctgttata
     5101 ttggacatgt tttttgtatt gccaacagca aaagcaacct ttcttgtcga agctattgga
     5161 aaagcaatca aagctgcggc gataatcacc gaaacagcaa ttgttgtcgt tgttgagata
     5221 gcacctaaag ctgttttctt catcgatagg cctatatgat taggagcata taacaagagc
     5281 gacgaattcc atttgtggta tttgactctc ctgggatgat ggctaccata tctatattcc
     5341 acttggggtt agatgattat tagcaattga aataatgtta aggatatgct gcactcccac
     5401 acccgcagcc ctgttcaatc tctacaactt cataaacact tgtccactcg atactcaagc
     5461 ctcgaattac tatcagacat cttagcttat cgtagttgct cttaaaaatc acaaattttt
     5521 atatcaatct aacggtctgg tatctggcgc tgaccaacaa gcaaaactcg agaagttggt
     5581 ggttctaatt tccttaatta atccggacgg catccagaca actcttatat tggaactgta
     5641 aatcaatctg tttaactcag ctgagcctgt gaaagattcc accatggact ctaagctaca
     5701 ctgctccatc gcgggcggcg gcacccgaca tccgtcagac tatcagacga taagtctacc
     5761 aattttgtat cttattggag caaaagtcaa ataaagtttt atgaactctt ataaataaga
     5821 gaagactatt tgcaagttta tgaataacaa aaccgcaata ttgactttaa tactggtgag
     5881 tgttgcagcg atatctacta cttcgatact cgttagttac aaagcacaag cagccgtgac
     5941 atcatcgtca acgacggcag ggacagcaac aggaacgaaa cctaatgccg aaattaaagt
     6001 tggaggaggt aatgcaactt atctgtccta aaaaaggtat ggtaatagat catgctttga
     6061 gcaaagctct gacgaatgtc ggttagtgcg ttaataagat ccgattagaa ctgtatctag
     6121 tggcaaattg tattggtaat cgaactggat cgaatatgcc gtcatagctt gttgctataa
     6181 tcggattcca ttcattatca actgctgagc acgctgtaaa aggtaagttc aggtcctgcg
     6241 tcactaccta tccgtataca gttgccctcc cacactctgt aagtagctcc catattttta
     6301 gcagtccaac cttttccata caattcacta tggtttgcag gaatctatgt gagaggttgt
     6361 actcccaaat tatttttggt aagagtcatt atgaaggtgg taaaaaatac tgtagaaggt
     6421 gtgaggtgta tttttgtcac aatggtttct tctgtccctg ttgcggtact gcattgagag
     6481 tgtcaccaac tcgtaaaaga gataaggcaa ggctgaggca attacatcta agaagagaag
     6541 aacaggatag aataatcagg attataaaga ggataaagaa gtagcctgtc aatcaagtca
     6601 agtcaaaata agataaatat atctagatta ttttacagcc actgccaaat tctaacaact
     6661 attcatgaag tctctgctac agaggcttta ctttgacagt tatcttaaaa cttgtcaaca
     6721 agtctttaca tctactgcag atctaaaatt ctgtaatacc ttgcaacgaa gctggtaagg
     6781 tattttcagt acaaaggaga atcgtcaaaa ggcgttgatg ttgtctccca tcaagaaaag
     6841 tcgtagacaa ccaaggaaaa tcaaataaga aagctgttca tcctatagta ccagcgaaac
     6901 tcatgccatg gtttgacacg aaaatgagtc gcaggaaatt cttgaaatta gcaggttctg
     6961 gaatagctat tgtggtagta ggcggcactg tctggcgtgc ctttagcaac ggggtattca
     7021 gtaccggaca aggaccagcc tatgaaccgt ggaatgactg gaaaacggaa tcacagcaag
     7081 gagcaggacc attatcgcta gtagcttccg ctatcctggc agcgaatgca cataacactc
     7141 agccctggct atttgttgta aatccaaaca gaatagattt gtttgcagac actaagcgaa
     7201 acatcggagc aattgatcca tacctacgag aaatgtttga agggcttggc tgcgcccttg
     7261 aaaatttggt tctggctgca gaagcaaaag gatatacgta taatttgaaa cttatgcctg
     7321 agcccacagg taagacccat gttgcaacta ttagcttgat atctaataga aagattaaca
     7381 atagcataag tggcaataat actaccacta ctctttcttc aaatttatca gaaccatcat
     7441 cattatacaa tgcaataccg cagcgtcata caaatcgtgg accgtatgag atgaataagc
     7501 ctgtaacaca ggatattctg caatcattag gatcgttgag taaaacaata agtccagaag
     7561 gagcgatgac tattttctgg tttaccaccg ttgaccaacg tcataaggta ggggatctta
     7621 tattgcaagc aacccaagcc attatagctg atgagcaaca atcatatgat gattttaaat
     7681 ggtatagagc tagctggcaa gacatccaga cttataaaga tggacttact gttgacacat
     7741 caggttcgcc ttggtacata gaggttttag ccaaaatact gccacctata tccgagaaag
     7801 aattcgacaa ctcccagcta cagttggcta aagacaccta tgtagccaca gcatctgcat
     7861 ttggaattat tgcagttcat gatgtgtttg acgatattca aaggttacag ggtggaatgt
     7921 tttatcagag aatgcatttg tgggctacat caaatggaat tgctatgcag ccactgaatc
     7981 aaatgactga acgtgccgac agagaagtga gtttaggaat caaacaaaca tttggaaatg
     8041 cgttaaggga actcatcgcc aatccaaact ggcaggcatt gatgacgttt cgtataggtt
     8101 atccaacttt agaagcatta agtagtccac gtagatcggt tcaagaagtt gtggtttctt
     8161 cggtataatt gctaattgag tatattaaag gcagtatttt tccacatata ttgatgatgt
     8221 ggtaactgtg gctgatagca gaatattgat gtccaaataa gtgaaatcta gtctacattc
     8281 gaaagatgag tcaattggga acttgctact ttatactaga ttgtttttac taccgatatg
     8341 acagaatgat ttatttccca gaataacgat ataatcattg tccttcattc ttaccttcac
     8401 gataggaatg ttggacacta acaaccgcat aagcgcttgc aaactggtta gtgttgccac
     8461 acacactaag acacaatgat cca
//
LOCUS       DAOR01000138            8275 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_54269, whole genome shotgun sequence.
ACCESSION   DAOR01000138 DAOR01000000
VERSION     DAOR01000138.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_54269"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1479..1620
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6602..6888
                     /estimated_length=287
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7134..7355
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7647..7762
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2280 a         1481 c         1355 g         2392 t
ORIGIN      
        1 cagaagagtg gaatacactt ttcaacttac tggtcgaagg taacaaactc tgggcaaaga
       61 ccgctgcatt gttagtcgtt gtaatttcaa ggaagaattt tgaacataac aagaaaccat
      121 caaaaacata tcaatacgag gctggttctg cgtgggagaa tttagcatta gaggcaacct
      181 caagaggact aagcgcacac ggaatggctg gatttgatta tgagaaagca aggacagatc
      241 tagatattcc agataacttt gatgtaatgg ctatgattgc tattggtaag ataggaccaa
      301 aggataatct gccaccgcag cttcaagaaa aagagcatcc aaatgataga aaacctttat
      361 cagaaattat aatggaagga cagtttagaa agcagacttt tttcctcaaa tagtgggttg
      421 cacacacact cacatcctgt attgtggtat gctttgaatt agacatatat cttctctggc
      481 tttcaatcca taatggccat attactaatt atgatcgaaa tgattgagcc tagctctcat
      541 cttcatctcc atcttcggct aatcgtcaat atggattaaa ttacagacga tgaactgtat
      601 tctttggatt tttcattctt gcagtaaaat ttgaccttgg agtcccagtg aaataggaga
      661 gggtaatctc gattttcata tattcaaaca tgtgagtgcg aactattttg taaatatatc
      721 ctttcttttg caggaattca caatccaaga aatgtagtaa taaacaactt tattacattc
      781 gcatgcatta taataatgtg gatattcctt cagaaactag agtaggagat ttcacatcta
      841 aaccaactga atttagcctt gctaggtgga gtgaaagatt ctttgcttgg ttgatagatt
      901 ttataatagt tagtattggt attgagatat tattttatgc agcaacattt ccattgtggt
      961 ttgacagcaa ccctgacaga tggtttcgtg gtggagcaag catcgggttt gttgtacgca
     1021 gtttaatatt ccttgcatac tggacgtatt ttgaatcaac aggcggtcaa tcactaggta
     1081 aaaggatttt ccatctaaag acggtaggca tttctggaca gactgtaaac gtcagagatg
     1141 ctttattaga aagttttggt aaatcgtttt tgcttccaat cgacttgatt ctaggatgga
     1201 tatttacaaa cgataaaaga cagagaatat ttaacagggc gagtaataca attgtgatca
     1261 agctgagatc ccaaactgaa gaaaattcaa gtagtaatgt gcattacaga aaagataact
     1321 gacaagcaac attctcaggc agcaacgatt atgtagggta gtcatgctaa atatgaagtc
     1381 cactcctagc aaagaacgaa aaaaatacta ccgattatgg atgccaattt taggagctgc
     1441 cgtagtaggt attattgtac ttgtacttgt acttgtacnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 acttgtactt gtacttgtac ttgtcagtag cagcatgcag cagcaagtcg gtccagcacc
     1681 atccatccga cctgctgctt ccccaaattc attagcacaa gatagtatag cattatttgt
     1741 taattcgttt gaaaaagtag taaatgaagc tcataatttg acccagagtt accaacatga
     1801 agctggaaaa tggaagacgc accagtatga caacaagaca atgatttcaa ttacaaacaa
     1861 ttatttactc aaatacgaga atctggtaaa tgaatccaaa gcgttgcacc cgcctaaaca
     1921 ataccaaaat gctacagatc tatatacaaa gagtcttgaa tcggagcttc aaagttatac
     1981 tcatttccgg aattatttat caacaaataa cactgctgag aatgaattgt ctacaaggct
     2041 tctgtcagac gcatttagat acgaggttga ttcgtttaag gagctcaagt cctccggatt
     2101 gtttacaatt gtgccataaa cattgccatg cgatatcagg ttccttggct cactaacaaa
     2161 aactctttac acgagatgtt gtgggttcga aatgatttgt ttttcgatga tttttaggaa
     2221 tgtttgatgt attgtttatg actctttaaa ctgtggagtt atatccccag ctttcatacc
     2281 atgtactttt actatatgct ctgtcctatc tctccaaagt tcttcttctg tcattcctat
     2341 agcaacataa tcgcagtccg tcactcctcc atcacgacat gctaatgaga acatatcctg
     2401 tcgtgtttat catcatgcat tccttttaat ctcttggaat attcgtcgaa tgtatcagtt
     2461 agatgtcaag ggtgatgact tacattgaca aattagtggt ctcactgttc atattctgat
     2521 aatggaacgg ttgaatattt gacttgctca attctctgat tgctttgttc cctcctataa
     2581 aatttgatac cctttcagga ttgcgagtta catatatttc tgctgttatc tcctccttca
     2641 acctgtaaga gtagattatc ttgtacaata ttatcatact catgcctgag atgtaagagc
     2701 acatcatcaa gatgcggacc gctcctctga taaattgcag taattatagc tgttagagtt
     2761 cctttttcaa cctttgatat ttcctattcg gataagtctt aactgcatct atgactactt
     2821 cagatctgct tgaaaaaccc ttttcatatt ttatctcgcc aagtttatta cataactttg
     2881 tagatagcaa gatgcttgtg actgagaacg tgactactac attgctaaac tggtcagaca
     2941 ctattgttaa agcctaataa ataaatccta catcttggta tcaagttaat aaggcatcat
     3001 aattagtagt ccacaaccaa ctgagataac gaaagtcatg gcaagcgaat cgtattgtgt
     3061 tcgaattaca taaaaaaagt tgtgggcaaa aagtgatcgg gaattggaga aaaaacgaga
     3121 aaatttacat tcaatgcgaa gaataactaa gctaaagtct gtcctcttgc ttacctccct
     3181 ttaccttgtt gtacaagtca ttgctgcttt atccactaga agtttggcgt tattagcaga
     3241 tgcaggacat atgcttgcag atgttgcagg acttgctttg gttctctttg ctatcaatta
     3301 tgcccgtaaa ccagcgactc tgcaacatac atatgggttc tacagggtag aaatattagc
     3361 agcgctgtta aacggtgtag tactgatatt aatttcggtg tatattttgt acgaagcata
     3421 taggagaatt tttgagccgc cagaaattca aggtcttcct atgataatag tggctgctat
     3481 tggattagga gtgaatttta caggaatgcg actgcttggt agcaatgggc atttccacgg
     3541 aggatctcgc ttagacaatg tccacgttga cacagcaaag gaacatgagg acctcaatgt
     3601 gaaaagcgca tacttggaag ttcttaacga tactttaggc gctgcaggtg ttatcattgc
     3661 tggtgtgata atactaacta ccagatttta tctggctgat ccattaatta gcattggttt
     3721 agcattattt atacttccaa ggacatggtc tcttatgaaa aagtcaatcc atattttgat
     3781 ggaaggagtt ccagctaaca tatcacctga agaagtaaaa aactctatac tggagataaa
     3841 aggagttaca ggcgtatttg atctgcatat atggactata acttctggta tcaatgcagt
     3901 ctcaggacac gttgtggtaa ttgatcggaa cagatcacag acagttctac aagatataaa
     3961 tgctatactt gaaaagaaat tcaaaataaa ccacgcaact attcaaatag agtcgtacca
     4021 ttcagaaccg gattgagtgt gttctcaata gctgaaactg gtagtattgc atattggcct
     4081 gttttgccat attcgataaa tgtgcacaca tcgtgactca tctacaccag caacaagata
     4141 ttaccttagt tcataaagga gcggtaggga ttgtcaatac cgtaacgctc aagccaaaga
     4201 agaacttagg gagtacatga caagaagctc ccagaggaac atcaggggta ttataagggg
     4261 ttaacctatg cgaaagttaa gtaatcagta ctaatgtttg ggaatcatta aaactaacac
     4321 aataacatag gaagaccaat cttatggatc gagtacattc tggactacag gatcgggatt
     4381 tacgaatggt ccagggcaag gacaacagta gcggctgaag tatttataag caaatgttgg
     4441 attatattat ttcctacaat gttgatgttt tgcagatcag taaccgaaat cgcttgtact
     4501 actgtttttt ctgcgtgttt ggcggtgtag cccttgccag gctggtctga catatatgac
     4561 gtagatacat agatggtttg gcacattcta aatgacgttt cactcctgtg agacttctgg
     4621 ttgtgacctg aaagacacaa acctttccat ctactccctc acttttaagt cgaagtaaaa
     4681 attaaaaatt gattacttct tcgatctatt ttctctttca acgtatgcaa ttacttgttt
     4741 attaatgaaa tcttcaaaaa cggggtcata ccattgactt tcttgtattc gcttccagtg
     4801 ctcctttatt gttcctagtc tgaagcaagt gcgcatccat tgtagccaac caaaccattc
     4861 attatcattg aggattttcc tttcatgcat atcataggca tgagaaaata cgttgaggac
     4921 atcaaatgag tatattgcat caggcgacaa tgattgtaca ttattgatta cccttcctag
     4981 ctctggacgt tctagcaaat gctcctctag tctgtgcact ttttcatcca agtcactgag
     5041 aacctttgtt tctatgtcta cagacagccc ttttatttct ctccttgaga aaaaaagcgt
     5101 tatgagcagt gtgcctacaa tgcctattgt ctgtgatata cttaacatgt ctgagattcc
     5161 tatgtctaaa accataagta atctctagcg gcgttaaaaa taagagtatt gaatggttac
     5221 gatactccca cagtctaaaa gtcttactag atgtagtatg taatcagtaa taacaaacaa
     5281 aatccattcc agcattaact ctttctatat ttaggaaaaa tacccattgt tactttgaat
     5341 atcgctttgt tagctctcca tgacataacg attagtcgtt tcctaataga tacctgattt
     5401 aacaaatctc ttgataagga agtaactatt tatgaaattc tcccttgcca ggtgactctg
     5461 gaattcattt atcacatgtg agagaagaat atttaatcga aattatacat tgtccagtat
     5521 acgcagcaaa agaatatgat ctctgaacca tgggaacaga aactccgctc ttctttatat
     5581 gtctagaaaa tatttgatac tgtatccttt agttctatcg ctactttctt tcctttttca
     5641 tctctacttt ggctgacata atgctctacc aaatcctgaa ccatcacttc tactacgcca
     5701 tctagagctg cgcgaacagc agagatttgc tgcactatat ctgggtagcc cttatcctca
     5761 tcaaccattc tttggatgcc tctaacatga ccttcaattc tagctaatct ttttgaaatt
     5821 tctggtttct tgtgatgcgt catatcgcct aaaattttcc taataccact ggatagttta
     5881 tccgatatgt aacctttatg ttgtcaatca tgttttaccc tatcactcgt ttaatcaaag
     5941 tttagttgtt gctatcaatt atgagcctag ctgaattcgt cataaaaaca agttcggagc
     6001 ttacgtgaat aattgcagcc agtaagggat ttattacacc aacaaatgcc agaatgaaac
     6061 cgagtccatc tacaaataga gttccaaaga agttttgttt gatggcaaac agagattgtc
     6121 ttgatgtcct tagcaaataa ggtatcttgg ttagatcctc agtcataagc accacatcgg
     6181 cagtttcaat agctacatct gttccacttc tccccatggc tatcccaaca tcggccttag
     6241 caagagcagg ggcatcgttt atgccatcac ctaccattat gacagcttca ttcttccttt
     6301 ctcctttctt tttcactatt tcctcaatta ttgaaacttt gtcttctggg agtaaattag
     6361 catggtattc ctcaattccc gcttcattag ctatcctctt tgctatgctt tcattgtctc
     6421 ctgtcaacat gacaacatga atactcattg ctttgatctt ggatatcgct tctttggtct
     6481 cttggcgtaa cttgtcttca agcaaaactg caccgataat ttgtctatct aacgacacaa
     6541 aggcagttgt ggaagagaat agcttatcat tatcattatc agaatcagaa tcagaatcag
     6601 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cagaatcaga
     6901 atcagaatca gacccagtaa tctgcaaggt ttgaccaaaa ttaccatcgt cttttgcaag
     6961 gtactgatat tgtcttctat tcagtaataa tgagaatcct ggtctcaaac ttgctgaagt
     7021 atctccacct gtatttgagc cggttaactt cgtttcatct tccatgaatt tcatattacc
     7081 tactgctatt cttcgaccat catggaatac agtaacacct ctacctactt ttannnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagta acacctctac ctacttttat
     7381 aatattttca ttgtcatcac tgctgatgtc tattctagat tgattgttac tattatccaa
     7441 attcatttca atttgtttct ctcgagctct tgctacaata gccttcgcga gaggatgatt
     7501 aacatttttc tcagcaatgg ctgcatattc taaaattctt attggatcaa tcctgggatc
     7561 aaaagaaaat acctgcgaaa catcgggctt tcctgaagtt aacgtacctg tcttatcaaa
     7621 tactatagtc cctgccctgt ttagaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnctatagtc cctgccctgt ttagattttc aatctggagt
     7801 ccacccttga ctatgacacc cctccttgag agcttaccat tagcagcaag aagagcaata
     7861 ggagttccga cagcaagtcc acatgctcct gcaactacaa ttactgataa agtagagaca
     7921 acgttatgag taacgataaa agtaagcaca gataacccaa ttgcgaattg aattaatctt
     7981 gtagcaagct tatcactaag cttatggatg ggagcttttg atgcctctgc ttcttcgacc
     8041 aaacgaataa tttttgcaaa agtagtatca ttatagagct tttcacattt tatctcgagt
     8101 tgctgagtta aatttatggt tccagcaaat gcagaatcac ctacactttt ctcagttgga
     8161 gcactttcac cagttatact agactggtcg actgttgacg cacctctaag aatatgaccg
     8221 tctacaggaa tagtatctcc ttctcttaca ataacaatat caccaagtct tactt
//
LOCUS       DAOR01000139            3343 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_19817, whole genome shotgun sequence.
ACCESSION   DAOR01000139 DAOR01000000
VERSION     DAOR01000139.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_19817"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1076 a          647 c          649 g          971 t
ORIGIN      
        1 atatctattg tgctacaagc taacatggac catgtaatga cataatataa cgatcttaca
       61 gcctttaatt gtaaactccc cgttgacgta gcctgtttta gcaaatcgta ttttgtacct
      121 gactgatact attacattta aaatcagtat cagggagtac gtttatgtct ctttctaatc
      181 caagtgataa caagccctga tcttcaacga cttgtacgat ctgcgggtac aatggctcga
      241 tagcatcgca acatgaaatc ctcctagcgc accgccgagt aaatttgggc tagaggtttg
      301 aataaagata tggggcaggc tattaaagca tcttcgattc tgggtctggg ttctttgatt
      361 cctgtccatg ccccaatttc ttgatatcaa aataataggc taacgtattt tcggccgata
      421 ttatcacaat tgtcaccatt acatcgagcg tatgtatgtc tactgtgatc ccaattacta
      481 gcctttgatg ctatttatcg aaattgtgca attgctggct tttaatggta ttttgcacca
      541 ttcaatacca atctatatat atatgattta gggcaatggt aagtatgtca acgaaaaaag
      601 aagcagaaag atccccctct gaatatgatg ttaaaagaga agtaagaaca ggtttatcgg
      661 agcaaaatca cgcagtcgat agagcattag atgaaaccag agataatatc aggagaacag
      721 tagatgaagc taggagagag attccacgta acactcaagc aatcaatgat tatcaagagc
      781 atactctgca atcagccaaa gaaattgcag aaagctacct agaatctcaa aagcagatta
      841 tacaatcatt tcagtcaaca tgggtcccgt atattgaaaa tatgtatgag gcatttagga
      901 ataattgggc ctccccacaa agggcagcgg aaatctatgc gagaacggta agcaattgtg
      961 cagataacgt gatggctaca aatagaatag caaacaatac aatatttatg aatatcggcg
     1021 catacaaagc atttgttcag cgtgagaaag atgatgtaaa agaattttcg aggatggctg
     1081 ctaacaccgc aagaacattt gaaaacaatt caagggaatc tgtgccaaat aataggcccg
     1141 tataaagata aaaagactaa cgtccacggg cgcaagtcca cttttttatt ttttgtattg
     1201 ctatcaatag cgagaagcaa cgctgaagta aaatcattga ttgaaggcat aacgaacaga
     1261 gctccgattt gtgataccat gtttatttga aggtttatac tatctttgtt aacagtggga
     1321 ttttagacta catacacaaa agaagcagaa atcaattcgg ataacagaac caccgattga
     1381 atttctatca agctagacaa aagtacctgt caatgcattc tcaaatcata taagggtgat
     1441 ttctaaatgc tatcgatatg gatgatttgc tgttactaat actgattgct tctattatcg
     1501 gtttgtccat tctatcgaca aattggtatt tcaagcccgt catggcggta agcattggta
     1561 acacaggagg agcgttcgtc atccaagaac aagtaaatac cggcaatctc gtcttagaca
     1621 aagaaattaa caaattctac agctgtattt cgaaaaccca tcaggacccg ccaatcatac
     1681 aaatagtgga caattgctat tctcaaaatt caatcgcagg aattaccggc ccaggcaacc
     1741 ataaccacaa taatagcaat aataataaca acaataacaa taacaataac attgaaacac
     1801 cttcgacaag gatcgctacg ccacctccag gtgttttggt acaagtccct taatctccat
     1861 acattggccg ctagcgagta ttgcggagga taagagataa tcatatttta cgctggatag
     1921 tcatgcattt gaacgaagct agctaaagtg ataatctctg tttgaatccc gacgcgttga
     1981 acgttctacg tcttggcagt tgacaataag ccgattcata atccgcctaa aaagaattct
     2041 agtgtgcaaa tgtctataat tcatattaag atatttgacc aatctccttg ccgcgctccg
     2101 ccaatgttca atcgacagat ttcgattccc ctgggggtct gcgttagcgt ggaatgctca
     2161 gcgataaccc tattgtggag cggtcacgga agtgacacac aaatcacagc acgttataat
     2221 gtaatttctt gtaatgcatc aataaatagc aatgttcttc tattatccct ctattgtcat
     2281 aataatcgaa tgagcgaaac cgccttctgt gactgataca ccgcctccgg taaaaagggc
     2341 attagtagaa gaattctttg actttcttaa gacatttggg gtaattggat tagcaattgc
     2401 ttttgtcata ggtcaagcag catccaaatt gattactgca tttgttactg atattataac
     2461 cccatttatt gggttgtttt tgcccgcggg ggatttgagc aaaatggcag ccactttcgc
     2521 aaaatcaaca ttcatgtggg gcactaaacg tgaaggaagg acctcacaaa gaaatgctta
     2581 aagtttggta tgattacgtc tttaagcgac agacattcga aggcaaattc gatgatgaga
     2641 caattggaca tcgcgttagg cacacaaata agtttgagac aggtatcaat acaagccttc
     2701 agcagttcct ttttcgggtt acattaagtt agcaattcga gtttaaatac cgtacatggt
     2761 gtgaagtctt tttgtatcac ggtggtgtgt attgtctttg ctgcggtacg gcgttaagag
     2821 tttcacctac ggctagaaag gacaaggaaa aaaagcaaat tcgacactag ccacttaagt
     2881 tataagccat ctcccagaaa agatattcat atttacaggc attggcaaaa tggcttttca
     2941 ttttagcttt agactcttcg tctactttct caccaagcct atttagaatc tcttttattt
     3001 cggccacctg cctatgtgat tcagttgaag agtagaattg cacccatttc ccgtaagcct
     3061 catttcgtat aggtatttta gacaatttct gagctatttc gagatatgtc caaggacaag
     3121 gtgccatgac tgacacaatt tcgctaaaag taccgtatga tgatgtattc attagataag
     3181 atatatagtt tacagtagtt ctacatggga agaattcata attagcttga ttagaaggag
     3241 agattgatga cacgcctaat gaattgagta attgtctttg tatcttcatc tcaaaattaa
     3301 ccgtgttcaa gtacaaacta tctaaccatt ccttcaattt gtt
//
LOCUS       DAOR01000140           16602 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_7729, whole genome shotgun sequence.
ACCESSION   DAOR01000140 DAOR01000000
VERSION     DAOR01000140.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 16602)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16602)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16602
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_7729"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    12894..13052
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16146..16454
                     /estimated_length=309
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4898 a         3169 c         2901 g         5166 t
ORIGIN      
        1 ggttggaata ttagtttcaa aatctggata ttgtcttgtt actgtcgtcc tcctgtccag
       61 ataatcaggg gtttcaaaag ttgctgcttg cagtgctttt tctccttcaa atcttttctg
      121 agtgaaaccc agagatgatc actgtcgtat ccttggaaat aatattttgg gatataatag
      181 tacttcttat ctaccgttcc ttcttttgtc tgattataat ccctcgttac actttgaatc
      241 ttgcctagat ctttgtcatc acttgacttt actttcttat caattgcttt atcccatgat
      301 ataatatcgg tcatcttcga tcctgattgt agtttcacat tatgcaattt atcagttata
      361 tttttaacct tagataacaa ttggcgaagc caacaagcct aataaaggtt ataaaataac
      421 gccttaatca ttaaaataca atgttttact atgatatact aggatattac tatgacaact
      481 gatagtaata gagcgggacc taccacatta tggaaaacgc tgaaaggcaa gaatgtaaga
      541 actaatgatg gaaaagacct aggcgaaatt aaggaggtgt ctgaaaacta cttacacgtc
      601 gaaaaaggaa tagtacgcaa ggagaaattc tggataccaa aatatgttgc agatgctttt
      661 gacggcaaga cactatggct tatgatggac gaagaagagt tacgtggaaa atatcaatac
      721 ggtacacagc caccacaagg cgatcaatat tcaaaagagt tcgaatcgtt caagggaaca
      781 ccatacggac aaaatgctag ctatgcagca gattttaacg ataatattcg ggttgttgaa
      841 aattacaaga atatcaggga tttgaaataa aggtagtatt ccgaagtatt gtcattacac
      901 ttcacaagca gttgagtatg taaaatcttt tagtatatca ttgacgttca aagtcatcca
      961 agagtctacg agtctaagta taggatacaa ctattgttcg gcgtaatcta agtatagcag
     1021 ctaacgtgta aaaaatatag gagtacatgg catgagtggg agaatatatg agcaagaaca
     1081 ttctccaatg tgttgtatcg atgccattta ggtcattatg atttcatata tctttcatat
     1141 cacaaataac tactaatcct ttgcattaat tagatgggag ccgttggcgc taaatgttcc
     1201 aaacttatct ttagctgctg taatcgcaga atctgtgtcc actccgttct cgtttcggaa
     1261 tattgattgt tttataataa attccagtgc ttctgcacct ttggttcctt ggcgtttaaa
     1321 gtctgggagg ccactctttc ctacgttacg atcaatacta aataatccga acctggcttc
     1381 cggtctataa ttttcaagcc attcataatt atccataaaa gaccaatgca agtatccgat
     1441 aacatttgcg ccgttattga ttgctcgttt gacttgttga aggtgtgcta caatgaacgg
     1501 agctctatac ttatcatttt tatcagcgac gccattttca gtaataaaaa caggtagtgt
     1561 gttgttccat tgatttttta ttcgcataat caaattgtat aatccttctg ggtagatttc
     1621 ccaaccgagg tcgtttagaa tgccatgagg ttgatgatgg gtttgtgcgt gaagattatt
     1681 tacgaatgat ccgccagcga actttgctga agaaagggcg agtatattgc tgtagcgaac
     1741 atatacacgt ctatagtagt taatgccaat gaagtcactc ttatttttcc actcctcgtg
     1801 cacgataaaa tccttgctat ctttgttatg cctttgaaga gtgttcagat aatttaggtc
     1861 ttcctctcca ctaataacag cattgataaa gtaatcattt acaaaataat caaagtttgc
     1921 agcagctctg atattatcat ttatggttgc ccctatcaat tttgttggtt ctgctggact
     1981 ggcagctacc attgcatgtg agaaaccaac cattttggaa ataccatctc catcggcatc
     2041 aatgctatca agagaagtga taatgtcata tgcttgaaca tgggcttcaa taagattgtg
     2101 taatactttt tttgctctat ttccatcaag caaaaaacca ggcggccata atcctgcaat
     2161 atagcctaca ccaatcacag atgctacagg ttcagctata gtcacccaat agtcaacgag
     2221 ctcttttagt tcttgtacaa tttttgtaac aaacttaatg aattctttaa ctgtctcgta
     2281 attctcccat cctctaagag atttccagta tggatctgag gcaggtgggt ctgcaagtgg
     2341 taagtctctt aatggtgatg gtaacaacct ctgacctatt ttttttgtaa atctgctagg
     2401 gggagttaat acccacaaag gaagtgttaa atggttcaac gtaatcacag ggttcaagcc
     2461 cctttctctc atagatctta tcatctgttt gtagccatca atagctccct gatcccattg
     2521 atctttctcc ggttcaacgc gcgcccactc tataccaatt ctaaaagcat tcatacccaa
     2581 tttggtggcc aaatcaaaat cattcagata gtaatgagga tcccaagttt tagcagcctc
     2641 accggcaggc cgtagcaaaa cttgggtact atctttgtac attagacttg gcttggttaa
     2701 cgaggaaatt cttttcttaa ttgattctga ggttgtaaaa taactccaat cattatttgt
     2761 aatccctcct tcaacttgat acggagatgt agagacgccc cacagaaaat catgagtttg
     2821 ttgcaatatt tcttctcatc acaataaaag atttcgtttt tatattaatc ctttgttggc
     2881 aacgtgataa cctgctgata aagaattagt tcatcaaatt tattctatct ttttataaca
     2941 tcaggggata ctatccttct gttttattgt acgtcagtat tatggaagtt ctacattata
     3001 gaatgttcgg attacaaatt ctacagcctt gtttgaaact ctgccgatat taatttacct
     3061 ttattcttcg gaaagtgtgc tcaatgtcag ccgcgatcag atcaatcctt caacatttgg
     3121 cgtccgactg gcaccatgta ctactatgtc ggtttctaat gtcgtgaata tttttatccg
     3181 cagtccttgc ttcaggacat tgaattgtta atcactaccc ctaaccaatc atccttcctg
     3241 taacgcacta tgaaggtttc atttattgaa cttcgaagag gtcacattcc tctatacctt
     3301 ttaatagtca aagaagatca ataacagtaa tcaatggacc ttttacacca taacaagaat
     3361 gtctgtgatc aatgtggcga aaaatttgag agttatgaag atctgatagc acacgcaaga
     3421 catgtacacc atcatctgat tgtaaaatgt catgagtgcg gcaaagagtt cattcatgaa
     3481 aaagatagac ttcatcatgt cagagaagag catcaggtaa aggtagatca tcgaaatcat
     3541 aaatgggaac atagtcaaga gacaaacaag gggaatccgc aaacggatct tgatgagcat
     3601 atgcgaaact ttggcgataa tttttgaatg gttattttat tttattttat ttcacttata
     3661 tgctatccgg agattttggt tgagtcgatt aacaaagtca actgactgat atgatttgct
     3721 gcttcctatt tactatcagg ttgatcgtaa caacgtgata atgccacgat aatcgaaaca
     3781 aaaccatgat tatatccgtt ctttcaaatc tatttgtaac cccaagattc agaaatattt
     3841 tgcataaata tattctagaa tagtggttcg gatttattag gccgtcgtca cagctaagcc
     3901 atctactaca tcttcttatt ctcctcctca gatttttctt ttatggtacc aaagaagttc
     3961 aacttttcat cactttactg ctttatgact agactagtaa tttttctgcc atctccttca
     4021 acgtaagatg tttcttttgc aatttcatta ttattggatc cacagagtta ccgacggcca
     4081 ggtttgcttt aaggtagaga gaaaaattga catatcgtgc gtttgaataa attgttaggt
     4141 tctttgctga acttatttgt gagttcgagc tagtcatggc ctattcggat tcgtaaaaat
     4201 tgggcaacga tcctcacctc tccccttttc ggtgtcatga tgatgatctg tgatagttgg
     4261 ttttctgata ttactcttct gagttctata cgtgccattt tctatcctta ggatccagga
     4321 tctacaaatc cttgacgtat tcgtctgaag tattcctcct gctcttttct tagatctggt
     4381 tcacgttcga ttctaaaagg atcgtctgct aactcttgta acctatagtc ttctaattcc
     4441 ttctgggtta gtggacaacc actactatct agcaagtaat tttctgaatc aaattcttgg
     4501 ggcatctttc gacctaacag accaaattga gggaattctg ttacagatgt tttcaattcg
     4561 cgctggaatt ttcttatacc ttttgcataa tagtccatat tcttggtatc tccctctgac
     4621 tttgctatct tgtatccttt ccaacatttg cgcaagccac cccatgtctc ggtatcatag
     4681 aatcctgaat ctagtaaaat tccatccttt cttgtgcact gctttttaat gcttaggtaa
     4741 tcgccggata atagattcca aactattatg ccttgatctg ttcttcctat atttcataag
     4801 tgaccacctt gatttatcat cagatctgtt tacatcgatc aacaattttt tatgctcttc
     4861 atccttataa catatagcaa taacatctga ttaaccgtag gtattcttct gcaaaatttt
     4921 aaaaagaatg gaactttatg cggcaccata taggcacact tttgacaaga agccagacct
     4981 ctagatatca cccaaaacta ggtgggttcg aaacactaga gacaagaatt tttccttgaa
     5041 attagatcca aaaatagcgg ctaggattaa tagggtatat caaatccgtt catccttgtc
     5101 tattgaggta tggttgaata aacctaaatt gaggagatct gtcctagatt ttagagacag
     5161 gaagacagat ttaatctatc atcgtgtatt cataataatg aagattacaa ctagaataac
     5221 aaactgcatt gcatttgaaa tgaagtataa ttggcctaca ctcacaggta atcttgtatt
     5281 gactccagat atcagcaata actagaaagt caggaatgat caagaaaacc cgccaaaccg
     5341 tcagatcaaa atgtggatgg aatatacagt atattccatc caaagtttca aagattttta
     5401 aacttattaa gacgtgttta tgtaattgcg gaattttgaa atcaaattaa caaaacttta
     5461 tgatagggtg aggagaacga ttattaattt taataaagga aggtgttttc tttttagaat
     5521 gcggtatgcg aaactctata ttgccttact ccgatatgat tttattctag catggagatt
     5581 ctacaagata gtaaactcgc taaatatctt tttgaaaaat attcatcaaa tccaaaaaat
     5641 tggaatttcc ttatctcgac aagcccgcta agggacggat ttttcgatgc gacagtctca
     5701 aatgttgacg aggtatggca actcaaaatt gactcaatct acaaaccagt acctattatc
     5761 ctcggggcaa aggttgatct cgacgccaca aaaatagaaa ggaagatgag taatagttct
     5821 ccggcgtttg gatatagaaa gcttgagcca tctctgatga tgaatattct aaaaaagtta
     5881 actcaggaac aacaaataaa tgcgaacaaa gaaccagata ttaataatta tatcaattcc
     5941 gtacttgggt ctctggaaac tgtcgctcca gtatcggggg gaaattatgc ttacggaccc
     6001 tttgtgttta ctgatagaaa tcccgcgggt tctaatgaat atcaaaaaca agttgccgaa
     6061 aaattagctc ttaagttacg tgaaaatttg aggaataggt attcaagcta cggttagcgt
     6121 cagaatagcc tgtattggcc agctagtgac ttattctcga caacattagt tacctagaac
     6181 cacttttcta atgattgagt acgttggacg attgattttt ttagtttctc tagagatcca
     6241 gttacccgat ctgtagaaaa gtttttttct atacaaagaa aatttatgat atcatcgtaa
     6301 cgcggttcag tgaactcaac accgtcagca tttgaaacct ccggttttag aaagatatcc
     6361 cgaatttctt gatatggaat atctgataat gtgttcttaa ttttctcaac gttctctaat
     6421 ttgccgtatt ccctaatcag ctttagacca gttttgggac caatgcctgg aacaccgcca
     6481 gggttaaagt ctgtccctat caaaattcct acatctatta attgttcctg ggtcaattta
     6541 gtttgatcca gaacctgttg gtgttcgata atttctgttt caacttcaaa atacatgttc
     6601 cgattaggaa cttttctctt gccactgatt gccaggttac gggtcagtct ttttgcccca
     6661 aacagtatcg agtcataatc ctggcttgca caggtatatg ctatacctgt ctttgttaga
     6721 tgggctgctg tggcctctcc ctccgagagg gcttgaatat aaggtattcc aagaaggttc
     6781 aaaatttctt tggattcttt tatcatttta tcagtcaaaa tagatgtagc ctggctatac
     6841 tttcgcgcgt cttcaagtct accttccgtc actgcttctt ggtatttttg ggacgcctcc
     6901 gcttttactt tacgtctacg ttcaatttcc tttgatttta ataaactggg cttgccatcg
     6961 aaaacgtaaa caagttttaa attgtcagcc aacaagttga tattacggta gaataatccg
     7021 ctcaaatggc ttgttacatc ccccttgttg ttcgtgagaa gttctcctgt tgggccacga
     7081 attgttgcaa ggaattgata gatagcatta tgcgcatcaa cagccactac tttatttgct
     7141 aattccgata tgactatagg tgacggcacg acaaacggtt ttagatctaa tcccattact
     7201 ttgatttgac tatagaagga ttttactttt tcttctgcgc tttgtccgcg atattattca
     7261 gcgtggcagg cccaacaatt tgtcggtaca cttggatgcc acataaccat cgtgaagccc
     7321 gtaatgagca atatcagact acctaataaa taagagatga caaagatgca agtagttaat
     7381 taataatttt ctttcagcct caaaatattg agcgctgaac ctgcatgaaa ccattctatt
     7441 tgtgatttat tgtacgaatg atgtaggttt atatcttctc tggttccgtc gctgtgctgc
     7501 atgtaacatt ttacaggctt cccaggttgg aggtcttgaa gtcctaagat acttattctg
     7561 tccccgtcca tcactttgtc ataatcatct gcattctcaa aagtcaaagc aagaacgcct
     7621 tgttttttca gattggtttc atgtattctt gcaaaacttt tgacaataac tgcaacacat
     7681 ccaagatatc taggagtcaa ggcagcatgt tctctactac ttccttcgcc atagttttgg
     7741 tctcctacaa tgatccgttt cacagccttt tctttatact ctcttgcaat ttggaggaat
     7801 gatttggtgt tgccgctaag actatttttt ccgattccta ctcctcctgt gtatgcatta
     7861 actgcaccaa ggagcatgtt atcactaatc ttgtcaagat gacctctgta cattaaccaa
     7921 ggccctgcag gagaaatatg atctgtggtg cactttcctt tcacctttgc aagtattggg
     7981 agattttcaa aatcctttcc atcccattta gtaaatggtt cgagcttctg taatctgcta
     8041 ctgtcatttt tgattactac agcaatccta tcaggctcat ccgatggtga aagataaacg
     8101 tcagtgactt cctcgaaacc atttgttgga atctcaggtg ctatcctagg aggatccagc
     8161 ttgaattttg taccgtcatc tgcggttaac tcgctatcaa gaggattaaa cgaaagtctt
     8221 ccagcaagcg ctagagccag gacaatctca gggcttccaa taaaattcat tgtctctctt
     8281 ctaccatcat ttcgtcctgg aaagtttctg ttaaaggatg tgaggatggt attacattct
     8341 cctcttctta gctccggtct gttccattga cctatgcatg gaccacaggc atttgcaaga
     8401 acggttgcac cgatcgtctt catggactcc atctgtccat ctcgttcaat agttgctctt
     8461 accatttcag aaccaggagt cacaagaaat ggctgtcata aatatgctaa ggggttaaat
     8521 gccaaaggcc aaccatttcc atcggagtcg attttatggt gctattattc tcacaccata
     8581 aaattataga atggttggcc tctaaaatat tggaatataa acgactggac aagaaaactg
     8641 aatggtagat gggtgactcc tgctaggctg ttcaggatgg aattatcctg gtattgcaga
     8701 caagggtgga tggacaggag tgtcctaacc tacatggata ccaaaaaact tcgctattat
     8761 tctcaattgt tcgacacagg agagatggac tctactttct atgacagatt ttattcacaa
     8821 atgacaaaag ctgcaattta aagccatgaa tggtatttcg ttatttgaca gtgaaacaaa
     8881 ggtactagaa catctggcta tccatgaaat aaacacgtct tctgctagac gtggatcata
     8941 aaaacttcat atatccattc tctcagtcgt tacaattatt attccactgc caatttgtct
     9001 atcggttcac ccgtaggtac cgtgaagcat aaatcattca agttcgctca cgtcttgaac
     9061 gcatagctgt aactagggct taccacattg ccgttatcta caataagata atctcgttca
     9121 atacgacgcc cattcataaa acggagaagg cagtctttta ttccgtccgc gtatctcctt
     9181 tgtgcttcca aagttgtacc ggagtaatgc ggaaccattg catgatttgg catacgccgc
     9241 catggatggt cctttggcgc tggttgagga aaccaaacgt cacctgcata gcctgccaaa
     9301 tggcctttct ccagcgcttc tacgagggaa agagtatcga cgatcctacc tcgtgcagta
     9361 ttgataagat atgcaccttt tttcatatgg aaaagaagac cgcggttaaa catgccgtct
     9421 gtctcaggcg ttaacggagt attgatagta atgatgtcgg attgttcgac aagttgatct
     9481 aaattagcat aacgtgctcc aagtgcaaac tcttctacag tgctcaaccg gtgatagtca
     9541 tagtatatca ttttaacatc aaatgccttc agcctttcac aaacccgctg tccaatcctt
     9601 cccactccta tgattccaac aactttatct tctaaatcat gtgcatttgc cgcaatttct
     9661 gcaatatccc atctaccatc aagaacctgt ttgtaagcag gtatgtaatt ccgcacaagt
     9721 gctaaaatat gcattaccac ttgctcagcc acacttacaa cattgctacc agtaatctca
     9781 gccaccgtga tcttgcgtgc agctgctgcc gccaagtcta tatgatcaga accaattccc
     9841 gcagtcagga cgatctggag tttagacgcc tttgaaattc tttcttttgt aacgtatgct
     9901 ggccaaaacg gagtagtaat caaaatatcc gtagtaggga gatgtttgtc aagctcagcc
     9961 tccttatcag tcaatacaac aaattcatgt ccttgatttc ggagaaaatc tgttaagcca
    10021 agggcattct ctgcgcaacc gaggagctcc ggcgtctttt ttgctatttc gcctccagga
    10081 taaagaactg ctacaatttt cataacttct aaagttgcaa aagtgattca aataaggttt
    10141 tcttagacag gattcaaaaa atattatagc ttgatcttat agatttatgt tcttaacttg
    10201 tactcttaga tttgtaggat gtctaggtta atgattgcct ctttttaaaa tatcctaata
    10261 tttcgtcgcc aacctgtttt gattcctttt catctgtatc aatttaaatc tcctctattc
    10321 caactcttat ctcagagata gacttgagga aaagaatgaa agaagaaaag aatgaatgaa
    10381 aaaagtcata atactgtttt acttcgctag ttctattgtc cttccttttc actaccactc
    10441 gattaataca tgtttctagc ttgcattcaa aatgacagat aatgaattat aatctcttta
    10501 ttgaaatgac ttaatccatt ggatcagttg tacgtgagtt tctgtgaaac aactttccta
    10561 ccacattttt ctttcctaat gcttcatcaa tggattggca ttagcattta ggtcagggtc
    10621 cattaaagct gaatcttcta atggactact ttagtttttg gctgatcccg tgggtcctct
    10681 caatatgata ggcattttat acaaaacatt taagatttga tatggtaatt cgtatgtaac
    10741 ttgagtatgc atgtaaagag tgctgattat ggatgtttac ttgatgagtg taaatctagc
    10801 ctatcttcca ttgagtgccc aaactgcaat tggaaactgc aagaagaagg atgttgttgc
    10861 tgtgctagta acaatgttaa tctgtgcaac taataataat attactctac ttgccactac
    10921 ctgaatattt tcaacgtaat ttctccctag ctttgttggt tattattcct atatttctcc
    10981 aaaaacacca tcataagcga gtcgtttacg ggtattcgtt tgaactcaag gtatccaagt
    11041 ttttggtata agtataaatt ccgagcactc ttgtatcctg taaacagttc atagcgatca
    11101 gcgaatttaa agtgttgttc aactgcttgc atgagtctag ttcctatacc tttgttctga
    11161 gaatctggtt tgacgatcaa tcgtccgata tatgcagttt ctttctcaag ataagctcgt
    11221 actgaaccaa ttatattccc gtcttcttcc gtggccttca gaaatacctg ctgtgaaaat
    11281 tcttgtttaa tctcctttaa tgtctgtatc aaaggaggaa ggctatagtc attatagatc
    11341 tccgcctagc tgagatacgc acgcatctgc aaatgaagaa ttgtctcgac atcctcaagt
    11401 gcagagctta taaaatatct tgcattagca ttgccatctg atgggaaggc tcatcaaata
    11461 atttcgagag aaaagtgtag aaatcatatg aatgaatttc atcaaatatt attgcatcaa
    11521 ttgaagcatt aaattgtgac aatagcagct gatgggctgt cgcaacgcta gttggactca
    11581 taagggtgcc aaaaagaaga cgtttggtca ttatgttcga tttattccat tttccatttt
    11641 cgccaatttt aagaattgat gttccatcta agatgccggt agtattgcca aaattttttt
    11701 caacctctag tacttggttg agacaacttc caacgcaaca ggcgtttcag ttagacaact
    11761 gatggagttg atcaaaggaa catatatagg aaattcgaat ctcccacctt gatgaatatg
    11821 actttttcag cttgcctgtg tatgattata ttatctatgt ttccgttcgt actccagctt
    11881 ggaacgccag ttaatattat aacaacacca cctctagttt atgcacaatc atgtgtgaat
    11941 agcagctcca ctactctgaa gccacttctg aatactggcg gtctttatat ttttcatcct
    12001 accaacgaaa gatctatggg tcaagaaaaa atttgctcaa ctccaagagc acaattgata
    12061 ataataaacc atttcaataa cacgggatgc tctacaggat gtttatcggc gtctaattct
    12121 acgataaccg ttttaggaaa taatccaaaa cccatcgcct tcaaggtttc aacaagagat
    12181 actaccgtca cattgggccc aggtcattat accgttgatg agcctagatt gtcagtattt
    12241 tatggacaag ttctcgcgcc tgattgctcg ggcatcataa gtgctgatgc aagtcctagt
    12301 gctgccaaga aatgcatcat aattaactca tattctaata atgttcagac atggctagat
    12361 aaaataaata atatcaaaat ccaatttggt tattcaccgc catacccatt tgtgggaaat
    12421 gttacacaac taaatttcaa ggtgtctagt gcaaacacta gcaaagactt agacctaacg
    12481 catatacata ttgcagtgat aaaaaatgta accgcaaact tcaataatag taacacaatc
    12541 aataacaaaa actattttgt cacatttgat aatctaactg cctcccatgg cattttctca
    12601 ctaaaatatc aattcttaca agaaggagca catcaagtca tcatcaagat agctgctaga
    12661 gatagtgaag ttgctttagc atcatttgat attccaatat tactgccaga gtaagataga
    12721 attactctat attgccgcta ccataataag ccctgaaacg agagcacgta ggtttatcgt
    12781 tattccggcc acataatgca gaaagcaagg tatatgctaa tttccttgcc tttagccacg
    12841 atccctagct aatcatttga atgcctgata gaatgcatag gttattgcaa cacnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgaacct atcagagagc ctatatttcg
    13081 ttctaattaa tacccagaag gataaattga gaaaatcgat tattattact ggagcttcta
    13141 gcggtattgg aaaagctacc gttataaaac tggtggaatc gggctatcag gtttttggat
    13201 tagcaaggag atacgataag cttgttgcta tttcctctaa attattaaca tcatcaagag
    13261 aagacaaccg agataattat attccaatgg aattcgatat aactaaacca gaaagattcg
    13321 gcagcatcat taatgatata tcatcaaagg ctaaaggagg tacaatttat ggtcttgtaa
    13381 ataatgcagg ctacgttgaa cctggagcaa ttgaggatat aacagtgcaa gatctaagaa
    13441 atcagtttga aacaaacttc tttgggctcc ttgaatttac caagaaagta ttaccattta
    13501 tgttggaaaa taatcaaggg cgaattgtaa atataagctc agtatcggga ttgatatctc
    13561 tgccattaat aggagcctat tcagctacga agtatgctgt agaagctata actgatgctt
    13621 taagaatgga gctatggaat acaaacatca aagtagtaac tgtcaatcct ggacttgtag
    13681 aaaccaatat caatacaata tcaaaaccaa aaattgacaa tctaatagca aacaaatcta
    13741 ggttttctga aatatataga aaatacctgt ataacgtacc aaaaggatgt ataggcgact
    13801 taccaaaagt gattgaatta tttaaaccaa ctttgtagtc ctcacagtcc gaactattag
    13861 cacagacaca acattgtata cacctactga attatttaca atgtctgtct aattatttga
    13921 taatattatg attattttta tttatattat ttttacttga tgatagtaac ataggactta
    13981 taaaaactat aaatagtcaa attgatattg agttgtatga gcaattacag acttccgata
    14041 agaatagaaa tactctcgtc tgccgagaat gtttcttcgc tcgataacga tcttcataag
    14101 attctggtag atgcgtctga tagttgtgaa tacacttctt ttgttgataa ggatggagtt
    14161 acacaaataa agatacaatg tactattact gaggaagaga agcaatatgc atggactcaa
    14221 ttacggttaa aacatgcaac cgactgctct ttgtcggcgg agccaagtgg agtaccagcc
    14281 aataatgcgc agtcagacac tcatactcat tttattcata aggttccagt ggatgacgaa
    14341 ctttagactc aaatttatgg ataacgttgg ggttcatgtc aaactatagt tgctgactga
    14401 aatagtgtca gaaagtagtg tctagtccat ccctcctgta tcgagaatgt ttgaaccttg
    14461 gccctgtcaa tactaaatga tatcctacta ttgtaagtag ataaagctgg ttttatcgcg
    14521 tatccccaaa aaccgtctaa aaacccgcaa ccaagtgcag tcacaaatgg cataagctca
    14581 gaagtcattg aagctgactc tgaactcctg tatgatttgg acgtcctcat gggagctcac
    14641 cacagacatt atcgattatt tgtaatgtcg cctccttagt ctaatcggga gcattcttca
    14701 tttgcacagc gttgctttgt ccctatattt atctaatcaa ggccttgcag ggagaaattg
    14761 gctatttcat ctatcactaa aatttattat tgccacttgt agcggacact gctgaatact
    14821 gctgagacga aaagcaggat gagcatcaat acaatttgca tttgaagcat tactccgatg
    14881 ctagttggtg gatcacgtgc atacataatg tgaggatgac ttgcttaggg ccatatatga
    14941 aaagaattgc ctatgattgt catcatatta atggtaacag tgataacact gccacagaaa
    15001 gtctgaaaat ttgtgaacga gcgatttaac aatgattaca ggaaaaagac ttgcatataa
    15061 tttcactttg attagatata tttggatttc gttcgctgtc tccaacttta ttacaaccat
    15121 cgtgagttct aaacaatgta ccagtagaag tggcaccctc agtatagtta tctaaatatc
    15181 atccattaac gggcaggatt actgctaatt ccgagcgatt cttgagcgcg tttaacctat
    15241 attcaaacac tttgatgggc gttgccagca ggtcaataga gcgttatgtt aagagcagat
    15301 aaattttact atatgattta tactaaacat ccggaattat gtgtagaatt tgaagtatag
    15361 atgccataca gatattgtag ccatgatact tgattcagcg gtctccggtg aaaccaaaac
    15421 gagaataatg ctgcaagcta acctcaatca tatccaaatt aagcgatata ttccttcatt
    15481 gctatcgcaa aagcttatca aacaaagaac cgacctagca gcgccatctc aagtatacac
    15541 aacaacagaa aaaggacaca gttttttaga aaggtataga gaagtccaat tgctagatgt
    15601 cacgctacga cacgataaga atgcatactg gaaggaataa aaataagacg agatcccgac
    15661 ctgacttcgt ttgttttgtg aaatacagaa gccgttcgga attatatgaa tacgtcgtgg
    15721 taatatgaca taaaagtttc aggcagtttt cgttgccaaa ttactcataa taccttaatt
    15781 tacgctgctt atcgatcttt gttctgatca aagaacgtgc acattctaat cataaataaa
    15841 aaatagatat cattgtacta attatttcgc gagcccccgc aaagctaatt cttgaagaaa
    15901 ttctacctaa caatctttac taaatatcta tatcgtcttc tgcttcttct tcttcgttaa
    15961 aacccattag ccttcttttg gttttgttgg ttttgcttta atccgtattt cagaaggttt
    16021 gatctcagtg gggcaatttg ataagtatat atctcctagt taccgcaaga catagcggca
    16081 gtatttaatt caaggttcta atagtttaca aacacaaact attaaattat attttgaaca
    16141 tcaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnaaagtc tttttaaact attaaatagt tacgtgagat tactaatgat
    16501 atagataaat gaaatgcaaa ctgttgataa cttcctagac tcactcaaat cagagagaac
    16561 taaagagcaa tatgcatttc atttaaaacg ctttacccga ta
//
LOCUS       DAOR01000141            6182 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_5926, whole genome shotgun sequence.
ACCESSION   DAOR01000141 DAOR01000000
VERSION     DAOR01000141.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_5926"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1983 a         1151 c         1217 g         1831 t
ORIGIN      
        1 gcagatttcc tgtattctac ggttgatact tacatagagg atgccaagaa agataataga
       61 caacatctag ttgaactatg gaatgaaatg aagagagata aggaaaagca tttgcagatg
      121 ttaagagatg ctttagaaaa agaggcaaaa gaacaaaagc ttaatcagta atattatgac
      181 tcagtacggg gcagtagagg taggtttccc agcgcgttta gaaatctttc tgatcactcc
      241 tatgatagct ggaaattatt ccaaagtctc attctctgag ctttgctaat ctggtcaggg
      301 tcgtaacgcc attcgcgtcc acacttgaat aagagatagt cctattatga aacgccaatt
      361 ctctgccatc ctgttcaatc acttattttg tttgcggcgt ccgttcggcg ttgaatacaa
      421 tgaaactacc aatcagagag gcaaaaaaaa atatgaagaa ggttatctga gatcgcttaa
      481 ttatattttg gttgtccttt ctgttgttgt actaatagca gctgatattc tgctatgttc
      541 cttcttgctg tgaaattcgc tgtgtatgtt aagttgcgtg gttttgttct tagtctatat
      601 ccgcagcacg gacaaaatat tcgatcccat tttaagaaga tctcacaaac ttgacagcgt
      661 ttctgtccag tagagtaacg gagtaatcag aatcaggaaa tacgtaataa gaccgaagac
      721 aggtttctgg ataaagtcgc taaggaagtg ggagataacc tcaataaagg tagaaagtag
      781 gaatgaataa tcatatagaa ttcttttcag cggatagtct gttcatccta tgatcccttc
      841 catatactta aactgatacg ctaggctcgc cttcacctca ataactaagg tcatctgcga
      901 cttcaaactt gattggagcg aactcaaggc cgcggacatc tcctatgagt ttggcaaatt
      961 cagccatgaa cccaccagct ttacagctaa aaatcatttg aagagcttct ccttgtagtt
     1021 cagtagctgt ctcatcgaaa aagaattcaa tatttacttt tgttttccca tctccagccc
     1081 aaagattgcc tttccctcga ggtatttaca aaagaaggaa cagactcgga agcatcgagg
     1141 aggcatattt gtcgaagcat tgatccattt tcttcatgtt ctaaagatcg ctactataaa
     1201 tcgatctgcc gtcgcgtggt gcttactgtt tatatcttaa agccatctgg atggcttgtg
     1261 ctgatttcaa ggagcagtac ttgcacaccg gctctgatgc tttacaacta atgcaatctt
     1321 ttatttttaa tttcactttc tattattact gaaaagtgac aaattcttca gtgtgcaaac
     1381 tagcatcggt ccttcttaat ctcattgtca gcatatggag caactggaaa aaggagctaa
     1441 tagtataatc taaaattttc attcttatat tagtgtctat aacaataaaa agatatgttg
     1501 tgcatctaaa tgattaatgt catcgaacaa tgcaagtaaa agagaaaata ttgaacaaat
     1561 tcctaaagac aatttaggag aagtacagaa agcagtgagt gaacaaggtg gagttgaagg
     1621 tcaaagaaaa gaagtaaatg ttggggatta tataaaaacc gcagcagtag gacaactgct
     1681 aaagggcttg gaatttcgag ctgacaagaa taaaatagtt cagtttgttc aacagcaaca
     1741 agaacggcca acaaacatct ccgaagaaag gaaacaagat ctattaaata cgctgcagga
     1801 gaagctcgag gaaggaaaac aatatcaaaa tgtttctcag gtcaccacag ctgcagagtt
     1861 agttcgacaa taacagtaga actctagcat cagaaatttg attatcctta tgctgctgtg
     1921 tatgccacta gaatatgcgt acatagtata tttttccatt actcttgacg tattgagttt
     1981 agctacacct ttatcggcta atagttcggg aatctttttt ttaaatgtct taatgtaatg
     2041 caaacctaac ctaagagatc aagctcattc aaacccagtt ttcattagta aggcataata
     2101 tcttcaaatg ttaagttcag ttatggaaga tcaactaagt ttaagaatca ctccaccgtt
     2161 gaccctgtcc ttgtttggct taccgggatg ctaaacgcaa atgtagcagc tttactgtca
     2221 gcattattct cagcccaaat ctctccacca tgagcatcga caatgcgtcc cggtcacaga
     2281 cttggtgcca aacttgagaa tagcctatcg cttatctctg gcggtatacc tacacctgag
     2341 tctataatac taacaataac ctcttcttca ttcttatttt caacaggagc agcagcctct
     2401 gtcgcttctc ttcttctttg gatatagaaa tagccccttg tttatctgtg aatttaattg
     2461 cgttgcttaa gaggttggaa ataacctgaa atattcgaac cttgtctgcc tccacgatgg
     2521 taggtcagag tcctaagcat aaacataagg tcatctaagt aaacataagg tttgacatta
     2581 tacaggaaca atcatagtca tctatctgca tggcaaatta ataatcctga aactgtttta
     2641 atccttagtg tacattaacc ttgtacatat ggaccaagct tatcattgta atgaatgcaa
     2701 tgtagatttt cacgacaaac gcatagcaac agagcatagg aatagtacag gtcatatatt
     2761 aaacgtgata actaacgaga aataaaagtg cggtctgtcg atccatacag cataaaatgc
     2821 agtttccttc tacttagagc gagcaattac aaacagttac aataccagta atggagatag
     2881 acgcatgagc actggcacca acaacagcga gaacaaggaa caagaccgat acaaaaatct
     2941 aaaaacagca gagataatcg ccgagacatt actcgattgg ggagtcgata ccatatttgg
     3001 cttaccaggc gatggaataa acggatttat cgaagcattg agaacaagac aagacagaat
     3061 aaaattcgtg ctagtcaaac atgaagaatc agccgctttt atggcgtgcg cctatgcaaa
     3121 atatacaggc aagttagggg tatgtgtttc aacttcaggc cctggagcca ttcatctttt
     3181 aaacggctta tatgatgcta aactcgataa tactccagtt gtcgcgatta ctgggagaac
     3241 atattctgat ctaatgggtt ctggatatca gcaagatgta aaccagtctc aactattttc
     3301 agatgtcgcg gtttacaata acatgatagt tgcgccagaa caagctgaaa tggtagtaga
     3361 tatagcatgt aggacagccc tatctagtaa aggcgtaagc cactttacaa ttcctgtaga
     3421 tattcaggaa aaagtccttg aaggtaaata ctcaaaacac aaagtacccg gacatacttc
     3481 tgataggtta attagctttc aggttattcc tgcagataaa aaaacaatgg aagaagctgc
     3541 ggagattctg aatacgggta agagggttgt tatccttgtt ggtcagggag cattagatgc
     3601 tgaaaaagaa gtaatttctg ttgcagaaaa attgaatgct ccaatagtaa aagcgctcct
     3661 aggaaaggca gctatcccag ataacagcca atataatctt ggtgtattgg gtatgttggg
     3721 gacagagcct gcaaccgacg ctatgaatga agcagataca ctactcatgg tgggtacatc
     3781 atttccgtat atagaatatc ttccaaaacc aggtcaggca agaggaattc agattgatat
     3841 aaagcctgag aagataggat taagatatcc cgtggagctt gggttattag gagactctaa
     3901 gacaatcctc tcagcattac ttcctttgct aaataaaaaa caggatgact tatttttaaa
     3961 ggaaaagcag aaagcaatgg acaagtggat aagtaaatta cggcagttca gaatcagtaa
     4021 taagacagag cgtgacattg cggaaaaagc tgatagtagt gctactgata ttggcaaacc
     4081 tggtatcaaa ccacagatta ttgcatccgc cgtttctgaa gaattagaag ataatgcgat
     4141 catttctgtt gacagtggaa ctaatacatt ttgggccgca cgctacatca atattaaaaa
     4201 tggaatgaaa ttttcattgt ctgggactct tgcttctatg gcgtgtggtt tgccgtacgc
     4261 cattgctgcc caaatagcat atcctgaaag acaatgcgtt gcctttgttg gtgacggtgg
     4321 attcatgatg ctgatggcgg aattttctac tgcagtacag tataagcttc ccataaaagt
     4381 gatcgtttta aagaataata cacttggaat gattaggtgg gagcaaatgg gttttcttgg
     4441 taacccggag tacggcgtcg agtttacacc tatcgatttt gcaggctttg cgaaaaattg
     4501 tggaggcaac ggatattcaa taaagaaact agatgaaatg cagcaggtta tgagaacggc
     4561 aatgcacgat aaagagaaaa agccaacatt aattgaggct tatgtagatc catttgagcc
     4621 aataatgcct cctaaagcaa agcctgaatt tctcgaaaaa atgtcaaagg cttttgaaag
     4681 tggacaacca tatgctcaaa ggataggtct gacattatac agaaacaaat ttgaagtaac
     4741 gtaaagtgag cataagtgtt gttagaattt tatgtattca taaaatatca tggtagcctt
     4801 aatgttgtat ccggacacag ttgagctaga tgggacaaca atataaaaat ctggatggat
     4861 aaggcaacca gtcataggaa gaaaagatag tacagacaac ttcaagtact aaatcacgct
     4921 ctgttactcc ccctcgttat ttttgaaccc ataataaact cagacattct tctctggcgt
     4981 gtcatctatt caaatctcac tcttccaagt ctttaaaatc catattagta agtaatttta
     5041 attaacgaag taattgatta tgttcaattt cttagtacta acaatcttag gtatatcggc
     5101 aactattttt gcggtttcaa gcacaatgcc tcaaattatc aagggtataa agactaaaaa
     5161 aatggatgat gtttcagcat ggctaatcat cgctttaatt gtcggactat ctttatggat
     5221 cttatatgga atagcaaaaa gtgatctggt gatagcaggg ggtaactcag ttggtgtatc
     5281 attgaatgta atattgctcg tattaaaaat aaagtattcc cgggatccta ttggctaagt
     5341 gcaaggtctt gatatatttc aggctttgcc gagattcatg aattttccaa tgcggtcttt
     5401 aaaatatcct cataaattga tatagctgct aatgcgccac tagctacagc taacactatg
     5461 taatgcctat cagtatcgac atctccagct gcttaaacat ttgcaactgt ggtttgctgt
     5521 ttattattta tttttacaaa tccttcatca agctggcagt ccagctgttc tgccagttgg
     5581 gtctggacat tgtagccaac gtggtagaag ataacgtctg tattatacag cttaccactc
     5641 tcacttagaa gattaatacg atgagacact agtctatcat ccctgatttc tatcaaagga
     5701 tcattttcaa caatatcaat agataatctc ttagcttcag ctatttctgt ctgatctaac
     5761 tgatgatcgt cttgaatgaa aaccctaatg ttgtcagttt agcctaaaaa ttcctttgca
     5821 tatgatataa ttccaccatt tttcccattg ccaattattg taagcttctt atcagctgct
     5881 tctaggccgt cacagtgagg acaatgccat gcgccttttc catctatttg tgaataattc
     5941 ttaatatctg gtttagtgtc tgtaactcca gttgctatta tgatatattt tgctattacc
     6001 ggagattcat ccccatcagt gcttacgaca aaatactgat tgcaattttc aattctttgt
     6061 actttctgct taattactcg agggaatgat actgtagaac atctttccat gccttcttga
     6121 taaattttct tggtgatata ttttcgaggc ccaggtaact gtggacatgt tttgtgatat
     6181 ca
//
LOCUS       DAOR01000142            3409 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_22111, whole genome shotgun sequence.
ACCESSION   DAOR01000142 DAOR01000000
VERSION     DAOR01000142.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_22111"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    273..477
                     /estimated_length=205
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    956..1211
                     /estimated_length=256
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1375..1632
                     /estimated_length=258
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          861 a          455 c          559 g          815 t
ORIGIN      
        1 agagagcaaa tttctttttt tcttcccccc ttttcattct tttttacccg agtatgactc
       61 tgcattatgc catgtgtctt aagatatgca gagtactata atattttata ataaccttca
      121 ctctttcaag gatattttca taagtagtgt attctgaaat cggtttcaat cagaagactt
      181 ttctgcattt tatatcattt tgcatatgat gtactttgat aagactgccg ccccaccata
      241 ttaccatttt agttagaaga agagtattgg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga
      481 tcaaaatcct ggtcattgac atcttacttt aacttagaac ctatccgaaa agtagagttt
      541 aattgagtta caagcaaact gtgtttgatc gataaagcta agctgtacta cactttcttt
      601 tgagctgtat tgatatcaga ggttactgga gttgattcct catactgcat acctgacgtt
      661 gtatgggata gaatcattac attgctatca tcatcaatac gtaggagaag gaagaaaaag
      721 actggtcgtc caagaatgga tgacaggaag gctatgagtg ctattttcta cgtcctacgt
      781 acaggctgtc agtggaatgc tctacctaga agtcttggtg cttcaagtac agtacatgac
      841 agattccagg aatggagaaa ggctggtgtt ttcaagcaaa tgtggatcga tggattggct
      901 atgtacgata aaaaaactgg gatcgattgg aaatggcagg ctatggatgg tgttannnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn naatggcagg ctatggatgg tgttattaca aaagcacctc ttggggggaa
     1261 aaagcacagg accaaatccc acagacagag ctaaatcagg tacaaaacgt agtattctgg
     1321 tagatggtaa aggagtacca cttggtgtat gtgtagatgg agctaacagg catgnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nngtatggtt atacaattca catcaggtgt agatggagct aacaggcatg
     1681 acatgaagat gaccaaagca acactgcaaa gcatagtcat ctatagacct gacccaacaa
     1741 tcagatcgaa gcagcacatg tgcatggaca aaggctacga gtttccagag gtatacgaat
     1801 tactggaaga gtatggttat acaattcaca tcagattaaa aggtaaggaa agaagcagca
     1861 ttaaagaagg aataccaaaa tacagagcta gacgatgggt tgttgaaaga acccattctt
     1921 ggatgaacag attcagacga ttgctgataa gatgggaaaa gaggattgaa aagtatattg
     1981 gaatgttaca ttttgcatgt gcatggatca cctatagaag aagtggactt ttcgcatagg
     2041 ctctaagtcg ttccttactt gtgaatccta aagaatgaat gtactggtta tttgcctgta
     2101 gaaaaaacat gctacaactt ccactctcaa aactcattta cgactaacag atgtaatgtt
     2161 tctttagaat cacgattcta cttatttgct gactgaagcc gacatggctg gtggctacgt
     2221 aatctaaaat aataacttat gaattaactg tcatgtgagt acagtcagcg aaatagcatc
     2281 aaacaaggag attgttataa taactgcaga taccggaaag tcagcttacg atgttgcaga
     2341 ccttatgaag aacaagaaag taggttcagt aattgtaatg gataaggaaa gccaaccact
     2401 gggtattatt actgaacgtg acatggtcaa acgtgtttgt ctgaaaaatt tagcagcaag
     2461 cagaataaaa gcagaagaaa ttatgtcttc tccgctaatt acgataatgt cttatgactc
     2521 aattgatact gcaacaaaag taatgactaa gaataagatc aagcgtctag ttgtattaga
     2581 agcggataat agaattacgg ggatgttaag tgtgtctgat attaccaaac acttggcaaa
     2641 aatattactc gatgattaca atcggtataa gtctttacgt tttgctgttg atctcactta
     2701 gcatgagatt gtgagtttat gttgttttcg tcaccatggt tcataagtgt taaatgacca
     2761 atatgcaaat ccagaaccca atgttgtaga ttgacctcct aatggattac catttattat
     2821 aatttgcaaa ctaggattgg tgttgtcagc atttagattt ttggatccta ttacccaaat
     2881 tttagggtcg ttgctatcat tcagaacgtt ggttctattt tccttcatac tttcacatag
     2941 gtagtactca agctctggct tcttttctct ttgaactttt gtagagtata ctcgactgcg
     3001 tgggttctat gtgcgaacct tttttgcagt gactacagga gaaaagaata agagctgatc
     3061 aaagacattg aaaataatct agaaaagtat cgtaacagct ccgcctaaca caaggtatat
     3121 catgtttaat agttcctcga tcgggagttt agcattactg tcaatggctt atctataaaa
     3181 gagctttatt accttatttt cttaacacaa tcaaggcaac atcacgtagg ataaaaaaat
     3241 atgctctttt aggtcaaaat tgtctttcgt cttaagatcc catcatgccg ggaattttaa
     3301 gtggagttct ataatcctta gtaacagtta aaccatgatc tgctgtgact gttacggatt
     3361 taagtgtaga attttctggc ggcgaaagca tagcattttc acccggcga
//
LOCUS       DAOR01000143            7266 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_62420, whole genome shotgun sequence.
ACCESSION   DAOR01000143 DAOR01000000
VERSION     DAOR01000143.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_62420"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    245..425
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2260..2520
                     /estimated_length=261
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2058 a         1392 c         1478 g         1896 t
ORIGIN      
        1 agcataaacg cgggtctatg agagttatat aatattgaca gcggtgatga catcaaccca
       61 caacagcaat aatcgcgttc aactgcgatt aggctacttc atgtgataat ttggtttatt
      121 caggaatttt ttttatctgt ccatatcttt catgagcaat gatttcttgc ctacaaatta
      181 tgaatctggt taagggttct gtttcaatgc gcattcgcag gtggattggg ctgcacttca
      241 tatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnngtgta taagagacag caattagggc cacaaaaaac caaaatactt ttattgaata
      481 ctgtgtcata aatcccaaat aagagcaggt ggtatgtgta gtggtattag aagttaagag
      541 acctcgtcca atgtgggaga ttctgttttc aactcatcac accgacatcg gacttttgta
      601 tatcataacg tctattacat tccttttcat gggaggtgtc ttggccctta gcattcgttc
      661 tcaattattt ctgccaggat tgcacttcat gggaatggga ccgaatgatt ttgaacgatt
      721 cttcacggtc catgggacca caatgttact tctctgggtc attccctttg gagcaggagt
      781 aggaaattat ctaatcccaa tattggtccg ctacaaggat atggcgtggc caaagcttaa
      841 tgcggtggca ttttggatga ttcctgttgc agctgcctta atttggctag gattctcgga
      901 tataggctgg accgcttatc ctccatactc tgttatccgt gccgctggtc ctgcaacgga
      961 catgtggatc tttggcatga agattctcgg tatctcatct atacttggtt cgatgaattt
     1021 tgttgtaaca attcttaaga tgaaacatcc tgacttgcct ctcatgaaaa tgtcgttatt
     1081 tgcatgggga atcctgattc aatcctttat gataattgtt gccatgccga cattttcggc
     1141 tgctatgatt atgatgtata ccgacaggct cggagtgtct ggtttcttca atcctgcaat
     1201 gggtggagat ccgatcgcat accaacactt attctggttt acgttccatc cagaggtgta
     1261 catattcttg ttgccggcaa taggtatggc atacgaaata atacccaagt tctcgcgaaa
     1321 acctatcttt agttatcaat caggagttac tgccttcgtt atgcttggaa ctatagggtt
     1381 tgcatcgtgg gcacatcata tgtttgcaac aggtatgagc tttacagaaa agaccgtctt
     1441 catgataggc actcttgctg ctgttcctgc gtcagctatg catgttttta actttgtagc
     1501 taccatgtgg ggtggaagaa taaagtttgc tgcacctatg ctatttacag tagggggcat
     1561 tatgctcttc tacgccgcag gtgccggtgg cgtagtgaac actgctattc cactggactt
     1621 tctaacacac gatagttact tcgtagttgg acacttccac ctatttatga tgggaactgt
     1681 tacgatgata ttcactggcg ttatctacta cttgttcccc ctcattacgg gtagaatgta
     1741 caacgaaagg gcggcacagg ttcagtttgt agtggcattg ataggcatcg cacttgtctt
     1801 ctctgtacaa cacctgttag gcttatacgg acaaccaagg cgtacattcg actatgtacc
     1861 tattcctcaa ttcattataa tgaatcaaat tgcgacaatc ggagcctgga ttgtggggcc
     1921 agcatacggg ttgatgctag ttaatcttat caagagcgca attaacggaa agccggtaga
     1981 agatagagat ccatttgccg taggggagct atattatgac tacgcacgcc gagagcccca
     2041 ttatgaaacc cattaacatt cgcgataggg gaacagtatc atgactgcgc ccaacggggg
     2101 ccccttcatt agaaccaccg gcaagaggat gtggcagggg ttggctatca tagtgggagt
     2161 gatggctgta ggtgcagcta tcgcggtacc aaattggcac aatttcgcaa agaatccgcc
     2221 acccgtgtca gcgatagcta caggatctcc aactgcggcn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 actggcggta gcagtggatc tgcgcctctc gcttcagctg tgcctggtgt taccaccatt
     2581 aatatcttag ccggggcaag tgtccaagga aatcctaatt ttgttccaaa cagcgctcaa
     2641 gtacctatgg gcaacaagat tgtctgggtg aacaaagata cggtaccaca tacggcaaca
     2701 tcgggaacta gtgcatcaga tcctacctca ggcaagattt ttgacacaaa aataattaca
     2761 aacgggcaga gttctccacc tcagttgctc aaaggagtca aagtaggtga ctcgatacca
     2821 tattattgtc aaatccatcc gtacatgact gctaaaatca cggttaccgc tgcttcaagt
     2881 tctagttctt caactagtag taatagcagt agttcagctg ccgcggcgag cggagcaggc
     2941 gctgccccag tacttagcat cttgcaagga gcttctactc aaggaaaccc tgcatacgct
     3001 cctgccacac tcacggttaa gaaaggcgat tccatacagg ttaccaacaa ggactcggtt
     3061 ccgcatacgg ttacaaatgg caaagatgca agcgatccca cttcgggtaa gctcttcgac
     3121 acaagtataa ttaatgcggg ctcgactgca caaatttcaa ctgcaaagct tacccctggt
     3181 gactatccgt tccattgtac aatacatcca tacatgaccg gactgctgaa ggtacaatga
     3241 taacggtctg ataacacgaa gaaatcatat gttactaaag actctatcaa ttgcgagcct
     3301 gtgttccctt tttgtactta tattcattgg tggctatgtg agtgcttcgg gagttggttt
     3361 gacttgccct cattggccat tatgccctgc cgggctcgtt ccgacaaatg aatttattat
     3421 agagtatttc catagatccg ttgcggctac gacagcattg ctggttattg taaccatggc
     3481 cttcacctta agatctaaat tatctcctag cggcatgaag atgtcgtcaa tgatagcaag
     3541 cgccgcagcc ataggccaaa tatctttggg cgctgcagtc atagtcgagc gacttcatgc
     3601 taccttggtt acgacgcatc tggggctagg actagtaatg ttctcgatga cgctcattac
     3661 aacaatgtat gcgtataatc ttccagctga agatacgaaa atacggttgc cggagccaaa
     3721 atagacctgt aaaatcattt atggatgtgt aggtgcatta taataaagcc gtgtaaaata
     3781 ggggtaagaa gtctcactta caacaaggga ctattatgcg cataccacat aacaaacgta
     3841 caagtacaca caatttattg aggctctggg ccatctcagt aaaatataat acatgtcaat
     3901 atcaccttac tttttatgtg gaaagttctg atcccaaaaa aaatcatttt cggcgaaaat
     3961 gccgccaggg attttgaata tccaaagaat tctcttgtca ttacgaccac tacttctgac
     4021 atttatgaca ggtggttggg gtatatgggg attaaaaatt acgaagtcta tgacagggtg
     4081 acgcctgacc cagctatcga aacagtcgag gcgatcaaga aggaatatga tggtagagag
     4141 ctttctgcct atattggttt aggaggaggg agttccctgg acgtctgcaa atatcttagt
     4201 aaactcacca acatacccaa aatactcata ccgacaactt ttggcacggg tgcagaaatg
     4261 acaacgtatg cggtcataag ctttgatcac cgaaaaaaat tgcttcaaga tgagcgcttc
     4321 ctggctgacg cagccattgt ggacccgtac tttttgtcgg ggaccccatt caatattatg
     4381 agaaattctg catgcgatgc ggcggctcaa gcatcggaag gctacgacag taaacatgga
     4441 aatcccctta caaaactatt ttgcaaagaa gccttcgata ttttggagga tgccataatt
     4501 aatgaaaagc ctcatctgtt accatacgga gccatgttat ccggaatagg ttttgggaat
     4561 tcatcaacta cattaggcca tgctctgtca tacgtgtttt caaatgaagg agtagcgcat
     4621 ggctatgccc tctcttcatg tactactgtt gcacatgagt tcaacaaatc tgcatattat
     4681 gagcgattta agaaaatttg ccagaagttg aagttcgaac ctttggtttt aaaacagcct
     4741 attgaccaag cagctgacat aattatgccc gatcacgggc atctcgacaa taacccagtt
     4801 ccggtcacaa aggaaaatgt aatctactgc ctcgaaaaaa tcactaagaa aaatccgcta
     4861 acgtgaacgg atctttcgtg catatttaaa aaaggacatc catcacgtca cgttaattag
     4921 aaataatcgg ccgagatcgt atccggatta ttatttttat ttttattatt attcactaat
     4981 actggtcatt ctttaatcgt gataaacatc atctggaaac ctttaaattc agtttagaga
     5041 tttctgcttt agtaaggata tataatgact accattagca agagtatgga aattaactcg
     5101 ccggtgagtg aagtgtttac gtattttgca aggccagaac atatggctga tcaatttcca
     5161 gaaaatatgg gtttgaacat tattccgatt gaagtaaaaa atggcttcgg tgtaggcacc
     5221 atttttagaa ttagtggcga ttttgacggc aaaaaactcg aatgggattg tgaaaccatc
     5281 gattacatac cacatcgtaa gatagttgct aagatgattg aaggtccatt taaggagtgg
     5341 caaatcctag tagaatttgc ggaggtcgac gaaaaaaaga ctaagacgag catcagggtg
     5401 gattatgaga tgcctatggg gcctttaggt ggactcatag ataaggttag actaaagaaa
     5461 gtagcagaaa ggggaataga gaacggtctt tatagggtaa aggcattgct ggagggaact
     5521 ggttctatcc cagtttatat cacattggaa gcatacagac aaattctcaa agaaaaagaa
     5581 agactccact gctccgtatc tgataccata gtcaatctga taagagaaca gcaacaaagg
     5641 gaaccaagac ctgcccagca ggtatcttga agcttcattt tcgttcgtct ctagtttcgt
     5701 ttaaatatcg ctgtaaaacg ttgagttgtg cggagtcggg gttcgtagct cagccaggta
     5761 gagcgtctgg ctcttaacca gtcattattc atgggttaaa atgtcgttca gccgtatccc
     5821 accgaacccg ccagtaatag gggttcatat atcagaattc acattataaa tcctgcagga
     5881 caaggagggt aaattagaaa atgccagtaa agctcacgac aaccatcgtc aatatttctt
     5941 cattatctaa ttctacaaat cgatcgttat tgtacgaatt ctatcaatac atgaaatcta
     6001 taggaacttc agataattat caaaatggta acctaaaaat aatgacctat tttgctaaat
     6061 tcttagattg tgacaataat ctctatcaat ttcataaaag agagcaaatc gttgcttttt
     6121 tggacacgag aataaaagat acacaaaacg atcctgataa aagatggatt agaacttgga
     6181 atgattattt gcaacgaata aaatatttct ttagatggct acacaaccaa aaagaaagag
     6241 aaaacaaagg attagagata ataccaactt cagactggat tacacctagc tttgcccaaa
     6301 tcaaggagaa aagaaccaaa cgaattagcc catatttaga aacggaattg tgggaacggg
     6361 atgagattct ttcaataatt aaatatgaac cgtacaaacg taacaaggct gctttatcgt
     6421 tactatggga cttgaatgca agaaatcatg aagtaacatt gcttaggatt aagcatataa
     6481 ggttaagaga aaaatatggt gaaggcgaag ttccatatga gtccaagacc ggtactggtc
     6541 caacattgct tacatgttcc tttccgtatg ttcgcgattg gctcaatgaa cacccattta
     6601 gaaatgaagc caatgccaga ctgatttgta atctggtcac tggcgcaccg ataaaacccg
     6661 acgctttatg cacgatgatg aaacagctaa gaaaaagggt aacccgtcta ctagaaaatg
     6721 gagcgatcac cgatgaagct gaacggttaa ggatagagtt tttgttaaaa acaaagaagt
     6781 ggaatccata ttgcattagg cactctgcaa ttacctcaga ctctgactat cttccagaat
     6841 atgctttgaa aaagaaggtt agatggtcta tgaattcaaa gcaaggttca agatatatca
     6901 agaggagaat gggggagaat ttgaagaatc agatcttagt ccacaatggc attatttctg
     6961 aggagtctgc cacagcccag agaaaaccat cagttcttag ttgtgcaaga tgtagtcttg
     7021 taaacgcaat ggataataag ttctgttcaa agtgtagtta tcctctgaca ccacaggctt
     7081 acgaagaaat caaagcgcaa gaagatgtga aattcaaagc gatggaccag aaatatgaac
     7141 aagacatgaa agctatacgt gaagaaatga atcatcagtt cagtcaaatt atgcttatga
     7201 ttcagcagag cctctcacat agatttctac aagccctgtc tcttatccac atctccgagc
     7261 ccacga
//
LOCUS       DAOR01000144            5798 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_46935, whole genome shotgun sequence.
ACCESSION   DAOR01000144 DAOR01000000
VERSION     DAOR01000144.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_46935"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1731 a         1174 c         1127 g         1766 t
ORIGIN      
        1 ggtaagagtg tgagattcaa gtgagagcaa ttccatcctc ttcttatcct tcattctgat
       61 caattgattc gttgatggga gtagatcatc cacttttatg gtctcgagtt gtggtaataa
      121 cattttatct actcggtcat ccaatactat cctccttgat tcgaaatatg atacttcatc
      181 aggagtcata gggtttgcta tgacatctgt cctctgaata ggctttaggg ccgtattgat
      241 gctagaaaga atttgtttca ttttctccat gcgtctcttg cttgcataaa ggtaccgttc
      301 atctatagta tcctttgcgg caagtatcgt gacacaaccg gacgattttc ttccggttct
      361 accccttctc tgtatgtatc ttatctcgct gggtattggt tcgtaaaaca cgacaagatc
      421 tacttctggt atatcaagtc cctcctcagc aatggacgtt gctactagga catcaaactc
      481 tccatttcta aatgattcaa ggacaaccga ttgttcttcc tgactcatcc caatatctaa
      541 ctcccgtttt gcttgcccca caaagcgagc cgccttaatt ccattttcag tcaaaatttc
      601 gactaccttt tttgcagtgt ctctgtaatg ggtaaaaaca agagcttttg gtctttcatt
      661 ggttaagcca gtagctttct tatcttgttc atgttgtcgt ttattaatac tctgaaaaag
      721 agagtatcgc tgtttcaaaa gttctacaag atatattgtt tttggatgct ctatttttag
      781 actatttata agtgtctgaa tctcttttat cctattgtca ttcagcagcg attgatgcgc
      841 ctttccacca tctttttcaa tcctgtttag gaaagctttc agcgatggag acccttggct
      901 ttctataagt tctgcacaat acataagtgt aagagctgac gactgttgca ccaaggcggc
      961 ataaagcggt ccccttagct cttccatagt aagctctagc ttatattgga tctcttctcc
     1021 gagactaata agatcgctct taaagatcca ctgcggatgt ttttttctca gcaatcctct
     1081 ttctatgaga aaccgaagtt tgtcttcaag catcgcacga aagattgagc ttatgtattt
     1141 gtactcctga ggaatagtga accatttcaa agttacatcg attggattga tatatggttt
     1201 tacatctcca tcctcctcac tcctgtattc tatttgttca accgataagt tattacatac
     1261 ttccaggatt cttctttttt ctgatcccgg gctggcagtc atagctagaa taaaaggatg
     1321 cgatgattgt tgaacatagt acctggctat tgaggtataa gcatagtctt ttacagctct
     1381 gtgagcctca tcaaaaacta gcaagctaaa ttcactcagg gagagtctat tctctgccag
     1441 gtcattcttg actacctcgg gcgttgcaaa aacaagtctt acttcttttc gattccaaat
     1501 ggccattcta gttggaggca ggttctttcc tgtaactgct gtcatgcaat cttcaggcat
     1561 ccttaatacc gaaaagaatg agttcatatg ttgagctaca agcggtttgg tcggagccat
     1621 tatcaagatt ctttttgact tgtaattata cagaaattct gcacaaacat gtatggctat
     1681 aaccgtctta cctagagcag tcggaagaat tactagggta ttcttatttc tcgcagactc
     1741 ggatatgttg ttttgatata ctctaaactc gatgctgtgt ttttttatca atgggtggtt
     1801 gaagtattga ggtccattct gtcgagtaag agcgtttacc actctataag aattgattta
     1861 tactattata tctagcttta aatattttga gcatgggtgt ctacacgatc cgtaacatgt
     1921 gctgagatat tttgacaagt tttaagatac gatagatcaa aattagtata tcggtcctgc
     1981 acttcgtctt tttcgagatc cagaatattt atctggtttc taaggtattc gactttgtct
     2041 tgcagacaag gtatttcact attgctagca agtttcaata aacttatgat ttcctattca
     2101 cccatctgga tcatttagta ttttgtgtaa tctcaagaag gagtggaagg tagtgttgaa
     2161 tttgttcgta tttctcagtt aatttatgcg tttgtctaag ttcccaaaaa tctcgagata
     2221 ttgcttgcac ctcatcggct ggaagacttc tacaggcgtg tctacaggag ttttaccttc
     2281 tgaagacaat ctcaaagtct gggttatatt cgattttggg ttggtatcat ctttctcttc
     2341 tagttgtcct cctacttggt tctttctcct attccctcca ataggatctc cagtaggatc
     2401 cctatttcga ctttctggaa ttcatcaaga tgccttctct tcaggtttac ttcaataaca
     2461 aattgtttct tttctaatgg atcgtcatat tttgcaaacc gaatgcatag ccaacagttg
     2521 gtttaagagt cgccggtctc ttacacagaa gacaataaac agttctctac aaagttaatt
     2581 gccttgcagc agcgaacttc atttagtttg tacattagat aggtgtaccc tcccgggagg
     2641 gcggcagcgt taattttgta tttttttatg aaaagtttta aaaattaatg ttgcctacga
     2701 gcgtacgaca taggcctctt gtgtctcatg ctgacagcta ccgaaagtta cagtttggcc
     2761 tacccaaaag accagggtag gaaaaaaatg acgtattaaa cttaatatcg taaggttaga
     2821 ctattgagtt tatccattag tgagtaataa tgttgatata gatttaccga attaggacca
     2881 ccttattgca gcaaaccagg cggtacgaca cccagtttca caacaaaaac tgcatgggag
     2941 aaaacgatga tcagattctt ttttgaaggt acaccaatag tgtaccagtg ccatgtcata
     3001 aactagaagt gactgaatat aaatgtgcca agtgtaccta taaatggatt aatcgcatta
     3061 atggcaaaga tggtcaaaag ccgataaggt gtgccaaatg caagagatgg gactgggaag
     3121 aaggatactt gtcaactatt gaaaagcgat tgagaagaga tttacttaga atcgaatctg
     3181 tagaaacatc gcatcccact atgtttggcg aaagaggatt caatattgca ccatcagatt
     3241 tatgcgctag ttttctgagt atttatccaa ggcctacagt acaggagctt gaaaccgtcc
     3301 taaatccgat aagttatttg ggccctcaca accattatgg tttccgtaat catggtggaa
     3361 cttgtaccga acaggtaaac tgcggtcctg gatggatacc cataccaggc agctatagat
     3421 acgacaagga gaaagccaat gagatgggaa caaaggaaga gatacgccat gagttgatgg
     3481 agcatataat cgattctaga gggggaatca taaatgcaaa ctctacacat tacgcatatt
     3541 tagcgagtag aaaggagata gccaaagagt cgagtgaatt tgagcctagc aaaatgtggc
     3601 atgccattgc agagagtaag agacaggaag aagaacaaca agggaagaaa ctagaagagg
     3661 gaacccaaaa gaagacattt aaataacttt taaagaacgg aattggcctt aaaagcaaaa
     3721 ccaatactag aagaaatggg aaagccgaaa tagcgaatcc aatcttgtaa aagtcctagg
     3781 ttaaatccca cagtattgat acctgaaagt tgacagatag aatatcattc ctcatagaat
     3841 cctgagtcca aggttttttg gttttgatag ataatcagta gatcaaagaa aagcagtatg
     3901 tagctataac aaaacaaaat caacaaagaa atcgtattaa ctcggtaatc accaaagcaa
     3961 aacgtcatta aatatctatt gttataacaa gctatacaat aattaataca ttaacctcca
     4021 aacaataccc agacttgaga tcatacagcg aaactacaat caagcaacgg ttataagtca
     4081 taaaatttac catatttcta attacttgta atgacaaatt tagctgcaaa atgggcacta
     4141 tttgccgttg ttacaatgag tttagtgttt atggctactg caagcaaggg tgaccaagtc
     4201 ttggctgtca agaaaagtag tagtattaag attattcaca aaaccccgat cacgactgtc
     4261 gccactacca catctcataa tctaatcaag ggtgtaaaga ttcttcaggt tcataccatg
     4321 ccctcaagtg tggctgttgg gaatacattc agcgtacagg gcattgtgtt taacaactct
     4381 acagctacaa ttacatttgc aaatggaacg tgtaattctc cggtatctat agattttaat
     4441 aaaaatgtca tgatggaaaa tcagggagcc gcctcctgta cgacggctac tccagtagtt
     4501 actctaaaac ctcaagaaca ttcggcgata ctaagtccaa atctttctgg tatagcctac
     4561 aaagccactg ctccgggaat gacgaatgca acaatctctt tcacttatgg agtcgaaatc
     4621 cccacaggaa aatctccgat tagtgataat atttcgaggg tatatacatt caatatccag
     4681 agtaatagtt cgcagccttc ttccatatct gctacgacca gtaatgccat ttttagtcct
     4741 cctggggcag ggaccttcgg gagcggatcg ggccgcttgt taacaataaa gtatccagac
     4801 agtaatgtag atgttccagc aggaagtcta atctccgtcg gtggcacatc ggccccgtca
     4861 aatgcaactc ataccaattg caatgttgca gtacaaataa atcaacatgg attcgtccaa
     4921 gcatcgcctc ggggtccaaa tggagcagga gattatacaa agtggatagc agtcaccgca
     4981 cctccaaccc aattaggact aaaccagatc gaggcacaac ttctatgctt tcccccagga
     5041 accgtgtcaa caccaaatct gataaaacat ctggttcata atgttacagg actacaggta
     5101 gtaggaatgc ccacagcttc acagccacta tcatcaccat caaaacaaac tgcacctacg
     5161 gccccaccct caaaaaaagc agcgataggg cacaggctac aacccatgat accgctaatc
     5221 cccgggcagt agcgaagaac ccttactgaa ggtagcgcct acaggtatag aaaatcgaca
     5281 tgcaggcatt ggtatcgtct tgaaaagttt ctgggacata aaataaaatg ttggctagaa
     5341 ctgttatttt gagttttcag aattcttgtg atggtaagat tcgtataaac cagtttgcca
     5401 agaaagtgag gatatgatag aaaaaaacca aaatacgtag atagaattgc attttgtgct
     5461 ttactatgtt tgtttgtcaa tctacttgct tttgatgact ggctgttgct tgccagagca
     5521 gcgagtacgt ctcgtctaat gatttatttc tattgtttaa tatgcctgcc tatattccat
     5581 tttacgcatt tacatgatca tgatgtcaag accgcataga tatcgattgt tgctaatgat
     5641 atgacgatga tagtagaagt ctagatttcc ctatttcctt tcttgatcta aatttcgctt
     5701 tgtattttaa attacgtgac tttgttctga gcctgtaacc acaacatgga caccaccatc
     5761 cgcctccact ttaagcggta atatcagcaa aggtgtta
//
LOCUS       DAOR01000145            5502 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_6551, whole genome shotgun sequence.
ACCESSION   DAOR01000145 DAOR01000000
VERSION     DAOR01000145.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_6551"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1468..1653
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1971..2102
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1801 a          880 c         1031 g         1472 t
ORIGIN      
        1 aagacacgca aaagaactcg tggtccatac tggaaatcat caaatatgta atgatgacgt
       61 aagaaacatt tgggaaagat ttgtgagcct catgtcaaca gactctcatt tccctagcgt
      121 ccaacctttt tcttacaatt ccctacgact tgtcgtaact tgtgtgaaag gctgtattcc
      181 aaaattattg ttggtaagag ccactatgaa cttggtaaga aattgctttg agtcgctctc
      241 tttatgcagc tggacctgaa gcatatctta aagtcgtgct tggcctatcc aatgacagtg
      301 tccgagtttt ggtggttggc cataacccag gcttggaaga attactagag atgttcactg
      361 gcaaagctca aataatgccc acttgcacgt tggcacatgt gaaattttct attgatagct
      421 ggatagaact agattacgaa accaaaggac aactagcagg tatatggcag ccacgcgatc
      481 tcagttaatt tagtataata atagatggaa aattggacga tgacaacaac agatgataaa
      541 ggaaataaag cattaggtgg cggaatgtca aaaagacaga tgccacagca gcaaataacc
      601 aatttcatag acgtaaaatc ggtagacgaa tactcgtcta agggggagaa gctaggaggt
      661 aaagtagttg ttcctaagac tggagtacca ggaatgggtt actattcagt ttgcgttgat
      721 acagaaaaca acagttttgg tatattcgag tcaaatgaga acgataagtg aaaataattt
      781 cctatttccc cttatgtttt tagtcataat tagctaagct ttgagagttg gaattgcgtc
      841 aagagcatat cttgtggaac aactatagtt aaattttatg ttcctagtag tgaaacaaac
      901 aatgtttggc catgaaattg aggatcttag aacaaaaatg gtgataataa tctaactaaa
      961 tacattggac tgaagggtaa ggctatttac ttacctaaat gtcaaaatct ctatgaagtg
     1021 gtggtaagag cttaggcagg aacgtcgaaa agacatacag actgagagaa gtgttcacga
     1081 tgcataatgg gtttgtatca ggtcgtccag aagtaccatc ttgttcagct gtaactaaaa
     1141 cactagaaac tatcatgtct ggtaaaaatc ttcgtctaac aattacactc cctcacccgg
     1201 gttggactat atctaataga tgttccaaat ctgaacatgc gtacctattt agaatcacac
     1261 caagctgttc aagcttcatt aacagaaaag aatctcgact gtctagcttc ataaatcggt
     1321 actatgattt atcataatcg ttacttacta gggttgaata ggaactgttg ataactcata
     1381 ttatatgtat gaaaatctag tctatcctat tataatcggc ttcgctacga cacttcttgc
     1441 acttgaagca gcatggcact ttacggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgaagca gcatggcact ttacggcatg
     1681 caaactccct gacaagacaa taaaaccgtg tatttacaag caggttaagc tagtcctatt
     1741 aaatgcaaag cagacaaaag aagattatgt gaaataagga ggaatacaga gaccagatgg
     1801 aaaaaggagg cgattggcca agggtagtat cgtgcagaat tcttgcggtt gatgatgaac
     1861 gcgacgttaa cacagcatta cgtgaagtcc tagaacataa tggattcaaa gttgattcat
     1921 ttgacgaccc tctccttgct ttaaagaact tcaaggccca attatatgat nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nngaaatgaa tggcttttca ttttacaggg aaattaagaa attagacaag aattgtaaga
     2161 tctgttttct tacggcagga gagatgtatt acggaatata ttcagatata ttttcttcat
     2221 tacctgctaa ttatttcata cgaaagccga ttgggaatga agagctaata agaaagatag
     2281 atgaaattat caatgatacc gcgatacgag ttagaagtaa atagtatgga gtgcgaaaaa
     2341 tgacactttg tatgacaacg aaatgttttg aacatacagg aaatctctcc cctgatgcaa
     2401 ttgccgtatc tgagaaaaaa ccattttgga agagaatatt gattatagat gacgattctg
     2461 atgtcactat aactttcaaa gcagggatag aagatagcaa taacaataac aatgctaaca
     2521 aaagaattga agtatataca tctaatactc ctgtggtagc attgtcagaa ttcaaaccaa
     2581 atttctatga tcttctttta gttgacatta atctgccaca tatgaacgga tttgaattat
     2641 gtgaaaagat attggctatc gatcttaatg tcaaagtttg tttcatgtct tctggagaaa
     2701 taaaccgtga ggcgctaaga gagatatatc cagcaataag cttaggatgc tttatcagaa
     2761 agccagtcac tatagattat ttggttaaac gaattagatc agaattggat taggtgatgt
     2821 gtgatgatgc cgatttagga gctgaataca tagtagcagc atgagaagag ggaggagcat
     2881 ttgaggtaat tgcctaacct gttagaaaga ataaattcta aaattctctt aaaaatcacc
     2941 tttctagtta taatagaaat tattctaatt gttggcagct tcggtgtgct tgcttatttt
     3001 cagtctcagc aatcatcttt gggaaattca atcaatattg caggcaaaaa tagatatctt
     3061 actgccactc tcttgttgca aactgaaaaa tatttagatg gatctcttga tgcttcacaa
     3121 ctaaaagttg ctctggatag tttgcaatca aatataataa cattgaaaca aggaggaatg
     3181 atttcagggg tcgatctaaa acctcttcct tcagcctttt tagatatgtg gaaaaccatt
     3241 gtccaaaatt ggaatgctta caagacttct gttatacaaa atatattgtt gataccacca
     3301 catcaagaag tcaaagcaat aacgacatca tcaccagcaa caacaataac taaaggaaca
     3361 gtaataggcc agtcgttagc aaaggaaaga ttagaatcta tggcatctaa cttggttagc
     3421 tcttcagata tattggttac acaactagga caacaaacag ataagaattc ccagaatcta
     3481 atgctattac aactcctttt tgcaattctg gttgtcagta ttctggtatt aatattctat
     3541 cttgttgcca gaatgctcag accaatattc gttctcactc aagcaacgtc taaagtagaa
     3601 aaggggaatc tcgacgtatc agtagaacaa aaaggtaacg acgagctttc tgttcttagt
     3661 cgatctttta attccatgat agccttcata agagactata ttaaaaaaca gagtaattta
     3721 acaaagcagc ttgaagcagc aaacgaagaa ctcaaacgca aagaccaatt aaaagacgaa
     3781 ttcattaata tagcagctca cgagttgcgc ggccctatac agccaatatt gggcctagca
     3841 gaggttcttc ggtatagaaa aagagatcga gacagtagta atcgtaccgt cagtaaacaa
     3901 gaagacgaca aactccttga cattatcatt agaaatgcaa agaggttgct acgactcgaa
     3961 cagaacatct tggatatagc aagaatcgaa agccagtctc taaagctaga gaaagaaaga
     4021 tttgatttaa tccaaaaaat actgaacgtg attaatgatt ttgggaatga gttatcgaat
     4081 gagaaaacac agcttgtgtt cacaccatca caaaaagagc caatctttgt taacgcagac
     4141 aaagttagaa tatttgaggt tatctcaaat ctattatgta atgctgttaa atttaccaag
     4201 gaaggaagga ttacgatcaa cacagaaaag aaagataccc aagttcttgt gagtatcaaa
     4261 gatactggct taggaataca cccagagctt atgccaaagc tattttcaaa atatgttaca
     4321 aattcaccta tagggacagg agtaggattg tttatttcta aaagcatcat agaagctcat
     4381 ggtggtaaaa tctgggctga gaataatgtc gatggaaagg gagctacatt tacgtttagc
     4441 ctacctatga gtgaacatac cccttagatc taggtgtatg gggcagaaat tttactatga
     4501 accgtgcgaa tttagctgct aatcatgata tacaaatagt tagcgtagta gtaacagtcg
     4561 ccttggccat catacttttt gaagtaatcc cagagagagg tgtttggtca attcctgtct
     4621 atgggagaag catatgcttg ctgttccagc ttctggaatg ttgataagaa gacaaagagc
     4681 aaaagaacgc aaagctgcaa gtagattgga tgtattgcat taatttacaa tccatacaaa
     4741 tcttgatata aattaaaata attgaatagg gtatcacaag attagtgcca agatttcttg
     4801 atgttcattc gatgagtggt cttgatgagg atatattgaa gaaactccag gactcacctc
     4861 tagatgcgtt taatgtgaaa catctgaata tactctacaa tcccgaggct gacaaatgct
     4921 tctgtttact tgaagcgcca accaaagagg atgttaagaa acatcatgag aaaatgggca
     4981 tcaagtgtga atggatcacg gaagtaaaga caacggccta aaatacattc caatatacta
     5041 tttttaatag tggttggtaa gacatgtatt atattacatt ttagataata tagatccaat
     5101 catctgtatt aggagcagtt gtttcatcat acccattatg aaaaccatca caagaaagcc
     5161 gactatgttc attatggcag acactatgat acctcatgcg atgcgtgata tcaaaaaaag
     5221 cttgatagtt tccggcaact cctcctatgc ctagccggaa ggaaaaaatc gatttgactt
     5281 aaaatttcgt tgattagact cagctatagt agttctagct cgacataatt taactcaaca
     5341 tgggacttaa tagtataatt aattgatact actagtagca atgcaatgca tatacgagca
     5401 tcacttttcg cgggacttgg accggatatc tacaagttct tcatatttta gagatgaaag
     5461 gattaaagga tgggtctgcg ctctatggat aatggagaaa at
//
LOCUS       DAOR01000146           17703 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_24035, whole genome shotgun sequence.
ACCESSION   DAOR01000146 DAOR01000000
VERSION     DAOR01000146.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 17703)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17703)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17703
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_24035"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    10751..11016
                     /estimated_length=266
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12854..13157
                     /estimated_length=304
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5304 a         3300 c         3275 g         5254 t
ORIGIN      
        1 atagtcaaga gattattatt tatgtccacc agctaaattg taacgttctt atagcaaaca
       61 ttggtactgc gcgtaaataa tgaagactgg tgtagggcca ccttttcgat ggtatattgc
      121 atagcattaa cccttcattt acactcacac cttatcattt attatttgat tggatttttt
      181 ctcctaaaac ttagtataat gtcactgtaa tcatcatatt ctatagaaca tccctcgtct
      241 gcggtccttg tgtgtcagga acgtgtccaa catcatcagg acctacaaca acaatctgat
      301 aatgaaataa acaccagaac tgtcagcagg actttcagaa caccttcgct aatagttaac
      361 cgatctaaag tttgaagacc gccaaatgcg tactataatg agagcttgga caaagacccc
      421 ccatctcggc aactgtccca ttgcagtcca cggtgaagcc gtgaaacaaa aatatctatc
      481 gatagaactg atgctgcttg tggcataaaa aatgacgaag cattataggt ttgaatgaat
      541 atgtgccttt caattagtac tgaaaattaa tctatctatc tatcgaaaaa ataaggcaat
      601 aaccatgaat ctaccataag aaaaaaaata ctttactgca cttgcacaac agaaagtgta
      661 gatgctatct agtacaatgg atacttgtac atacagatat ttagaatcga tagaacaacg
      721 aaatagtatc atataataga aattgattta aactttagta ctaagtttgg gctctcataa
      781 ataattggtt tgactctagc aaaatatgaa atacagaagc agtagcaaca ttggtcatgt
      841 tcaaggcggt gcagtcggtg gcgcggatgg ggagtggctt tgtcccaaat gaaaacagca
      901 ttatttttgc tagtaccagt actggtgtta gtcttagcgc cattaacggt agtaatgcca
      961 cgcgcattcg caaccggacc atacgacgac ggatacaata cagcgaaatt cgacttctta
     1021 caccatagat catatagcga ctcctgccac accgattgta ctcagtacaa attgggatac
     1081 tctgaagcat ggaatacgtt aaatggctgg ttaggaaatc gttaagcgtc cctgaaatcc
     1141 gaactgctgc tcggcctgtt gaatctgagc aggagtttgg cccacttgtt gcaaggtaag
     1201 atattacacc ggctggaatg tggcgcactg gcgtggttag caatccctac atgattcatt
     1261 tctcagctga gttcactggg gaagcttcta ggtcagtaag atggggcgtt gattcgtcgg
     1321 gctacttgta gacctttcat agatagagag gagcaaatgg tggaggagct actagaacaa
     1381 aattaatgta taaagcgtat atgtcttgct cccccagctt acggataatc actacttaca
     1441 cgagatgcca gttgttacag gttaataatt tctgaagttg tattcctcat gtgactatgt
     1501 ggttgacata atctattaac atgacagcgt attgttattg tcattgaatg caaatcaaaa
     1561 taatatcttg gcaccgtcta ctactaatat tataaaaaat tgaaaataac tctactttat
     1621 cagattttgc ttcatcattt tataaaactc ttcatcagac ttgtttcttt taccttccat
     1681 ccaggtttct atatcctttc cagctaaata cctgagacta ggattttcac ttgtaacagc
     1741 tttgagcact acttttgcta caaggtctgg agaagaccca ttttccatca tatgctcaaa
     1801 gcttgtagcc attttttgca ttatctgcga atatggagag ttaggatctt gagatttctt
     1861 ggcaacaacc atagcattag caaagtttgt tctaataact ccgggttcta ctaggactac
     1921 ttttattcca aatggctcca attcgtaggc catagattca ctcaatcctt caactgcgaa
     1981 ttttgtacta acataagcag agcctcccgg gtaacctaac ctgcctgcac ctgaactcat
     2041 gtttacgatt atcccgaatt cttgcttcct cataatggga agaattgctt gagtggttct
     2101 tattactccg aataggttgg tttcaaactg tgcctttatc tcatccgttg taagatcctc
     2161 aaacgctcca ttaagtgcat aacccgcatt attcaccaat acgtctattc gccccacttc
     2221 agataatatt gcttgtactg cgttcttgat agatatatca tcggtaacat caagctgatt
     2281 tatatgaata ggcaagtttt ctttcgtcgc aactgatttt atattctcac ttttattcag
     2341 attacgcatt gtagcatacg tccgaaatcc gcttcttgcc aatgctaacg acgtctcatg
     2401 gcctactccg cttgaggtgc ccgtaactac agcaactttt tgattagatg tcatcttatt
     2461 ttctttttaa tatttagtaa tacataagac tacctttcac aatatgtaac aaatcaataa
     2521 tactgttgat acttttgtag aaatgacgac aaagtagtat tagttaatat tggaaaactg
     2581 ctctaaatag tagactagtc actctggttg ggataattat gattacccga aaagctagtt
     2641 tttctcgcac acagaagtca atcatggtaa atcatacggg tacgtcttat tagtactcgt
     2701 ccaagagaac tggagaaaag tattctgcat tcaaggtatt ggtagaggtt aaacaagcaa
     2761 tatcttggga tgctgggtat tgaccttttg tagtaaagga taactgaaga aaaattaatg
     2821 taatttacaa taagttatat caatgatcct caaaggcaag gaggacaagg gtttgttatc
     2881 aatggatacg cagcacaatg gatcaatatt tggaatatcg ccaattgttt tgtgtttcgc
     2941 atcttttggg ttagaataca aagaaagaag taaattcact tcgatattac tcatagtagg
     3001 aggagcaatg atctcaacat tctgactaat ggtaccagcg atgggtttat tcttgttctt
     3061 cttctatatt gtgctttgaa atatctatct acagattgtc ctgggcgcta tcataatggg
     3121 actaggtgta ctgaaagtgt ttcaagaaaa gaaattcgac acatacaata ggagagtacc
     3181 aaactagagg acgaacagaa aaaatgcaag agatgatgga tcctataagc ggatgaacaa
     3241 gaagaggctg tcgaaggact tgcaggtagt aggaaatatt gtagctactg atgaagagta
     3301 attcataatc ttgccaggtg ctccctacga atatagtatc ccgaaatctc gagcagacag
     3361 atcgcgaggt aatgatgtct taccagactt tcgtctcgac gatttattgt tcgtggagtt
     3421 agcatctcta agcaagacct agggatgtga acgagatatt aaggtgctaa aaaattgtaa
     3481 gaaaaatatc atggatccta tagtgaagaa gggaaattga aaatgatacc ttgcttttgt
     3541 aacgcaactg tggtgatatc tattgctgat atggccctct aatgtcgagg caactcatat
     3601 tccaaacata atggaatgga tctgattatt aaataattaa gaataaagat catctcactt
     3661 ccgttagtcc gaaaataagg taaagatgct aatggcggta tctatcgcca ccagtcctgc
     3721 aagaatatag tatctcccca tgcggtaacg atctgtccct ggagttcgaa aaattcagag
     3781 tagctcaact tcctgaaacc cagctgagtg gttcctgaaa tgctgtagcc atatcgacct
     3841 tgtcgctaga aaccgtgatt tcagagtgaa cggatactcg ggtattgttg catcatgtta
     3901 cgttctatga tatcaatata gtgaacggtg atctatgtac atccatgaca taacaaatat
     3961 ctactcgtac ttacattgta gaacacgcag gtaaagtatg ctcgtctgcg ttgcgtgtaa
     4021 aggtactggc ttaatcttca aaatagtgga gaacgaggca gggaagtcct ttatcgaaga
     4081 atgtcctgac tgtaatgatt tgtagtatct gtcaattcgt gcttatggtc ccatagcctc
     4141 cgttagtcta ttaaatatcg agttgttatt ttacattaaa atattcatac acatcctgaa
     4201 ccgtatttct tcgcctacta tgctggttta gatgaagagc cttattcttc taagaaaccc
     4261 ggtaattgct ctattagacc gatgtattgg ggttagttga tcccgataaa cttttgttcc
     4321 aacgagacaa attgtcgtag tacttaagcc taggcgctat agtctatata gactataact
     4381 aattatatag tagtgtttta tatattttgt tcatgtcaaa aactacatct gggactgcgc
     4441 tgcctaccgg acccaatgat gaacagcaga acttaccacc accacaagag cagacagaat
     4501 ccaaactccc aagaaatgaa ttaacattaa taaaaaaatt accacagcaa attaaggaaa
     4561 tagttgaaca aaatgctaga atagaactcc acctaaaggg cattaacaag gacattaaac
     4621 agatcagcca ggctcatcct gataaagcag aaagcagaga atcaatcaag caaatacaat
     4681 cccaaatagc gcagctacag acacatgtaa caaagattgg aattgctgtc gacaatgcct
     4741 ttgcaaatat aaagtcaaag aaaaagggca agaagaacgg caagaaaaac aagaagtaac
     4801 gagctagaca aataaaagca agtgctgttt gatttggatt tatatctatt aagacatggc
     4861 gactcaggca agagaatggc tgttgctgct ggtgggaata cttgtgacgt accgctcaca
     4921 atagttggca gagaagagat tgcaattata gctagatctg ttaaagccct aaatctgaga
     4981 tttagcgcta ttgtaacgag tcctcttaaa cgtgcagtac agactgcaaa gataattgcc
     5041 aaagttttag ctatcgaaaa cagaatatca gtttggaacg atttggttcc cgaaggcatc
     5101 aatcaaagct atataataga ttaaatcaat atacaagaga ttcaagtgtc ctgatgattg
     5161 gacatgagcc atacctcagt aatatcatgt atgatatgat atttcaaaaa aatagagtaa
     5221 accaacttgg ccgaataagt ctgaagaaag ctggattggc taagataaga gtaatttcgt
     5281 tgactccaaa tataagtggt gaattaagat ggttacttac gccaagaatc ttgaagttat
     5341 tagaaaaagg tagttcaaaa tcaaagtcct ccgcggcaaa taagaaaaag gaaaaggtca
     5401 gcggaacaga caaagtttga gaaagttctg tacagacatc tgcgcgttct acaaagctcg
     5461 caaaacgaaa gattcagcat gttattacaa tcaaggtttt aagcgctgtg tagaatgcga
     5521 gatatttttg aaatgtggag gtatgagatg tccttgttgc gaccgcattc tacgaataaa
     5581 accacataac aatttatcta aaggaaaatt gcttcaaggt ctaaactata tctcttgaga
     5641 aaatcgggta atggtgtctc gtatactttc gtcttaagga aagtgtgcga gagagagaaa
     5701 gtgtacgaga cgttggtttg ttctcttgaa tatattttag ctcttcaatt tattcattag
     5761 ctggattata gatataacga aaaaatatag tgggaaacaa ttgctttgta attggtgatc
     5821 gacactcttg aaattattta cccaaattta gcttctggtg agcttttcaa taatcgattt
     5881 gtgttgaggg tacatctcat caagttcttt tcttttcgca agttcaaagt cccgataatc
     5941 aaaagccggc gaacatgtgc atcctactag tgtataagaa gattggtcat tgacagtcgc
     6001 accaaaccaa gagccggctt ttactaatat ctgaagatat tcaccatttt ccaaattgtt
     6061 tcctaacttg atcgccgata agtcagcatt gtcatttatt atatacaagg taagcgagct
     6121 accatcgtaa aaatgccaca tttcatcaca tcctagtttg tgaaacgcag aaaattgtcc
     6181 ttgttttcca tctagcaaat agtatattgc agatgaaagt gaacgatcgc cattcagttc
     6241 taattgaatt atcttgtcag acctagaact ctccttgaaa tatccaccct cgtcatgtaa
     6301 ttcagaaaga tggagtttct ctatccaata tttagcattc ttagtcatcc ttaattttct
     6361 tcccataatc acaaagatat gggttatgca attgttgaca ctcaggatta ccacatgtaa
     6421 catacccttg tgttattgag gcgttatcgg gaatatggta ctatttttat catcagtcaa
     6481 acatcaaata tcattgattg tttgatgtta gctatattgt ttgtggtgat attgttaatg
     6541 actgttcttg tcttgtggaa ctgatgtgga atattgtaac cggccagata gttcgtttta
     6601 aggcttatgg tcactgaatt tgaagaatcg ctagcaagta tgttaataat ggtttgatgt
     6661 tctatcttag gaatttgggg ccttcgattg tcatcaatct tagaacgaac ggacttcaag
     6721 cgtaattttt ccaagaatat gcttatacta aattgttgaa accgatcatt ttctacgatg
     6781 ttcatagata gattctcagg tggttgctac ctttcgcgtc gattatcgaa gttttcaggt
     6841 gtgggataga taatttacct cgatggctta tgagttacgt ttctatgatt atagatgaaa
     6901 atttaacagc tacatctatt ggagggcttt tcgcaggtac aatctacttc ctcctctttc
     6961 taagcccata ttgacgcttg aaaaatatca tgtatgctga aagccaaaga ataaaccatg
     7021 gaagtttcag gtacgtctag aatagattct tgtctttatt attatctacg cctttatagt
     7081 aaaagtggta tttgcagtag ataattgcgt gttgttacta gtattgtttc ttacagatga
     7141 cgtttgagct ttgtaaattc caggctcaac ttgatttgcg ttagtgtcct tttggtccca
     7201 ctgaaatgtt tttgattcca ttggttttag ttcagatatc acttgtgctg caagtaatcc
     7261 tgccttttgt ccagtcttga cattttcaat attcaaacct agaagtgaat caggaaattc
     7321 taatggaaac ctcaagttgt tttttatggt gacaatgact gtttctcctg gaacataggt
     7381 catcttattt gtagatattt gcaaagtgga attgttggta ccggtgttat tacgactagc
     7441 aattgggttt atttgatttc ccgaagatat actattattt cccgcatata ttacatgcag
     7501 ttgagattcg aatatgagag taacagacaa caaaagaact ggtacgatac ataacgcctt
     7561 ttggttaacc attgatctta tttgaagaaa agttcgcgtc atattaaatc caccaccact
     7621 gtttcatttc ctggatctct aaaataaacg gattccgtag tccctgtctt cgtgccgcga
     7681 ctaattaagg tcttatttct gctagccgtg tgtgaagctc ccgatgtaca gtaaaggttc
     7741 gttaccatgt ggattgttga aataaccagg accaccccca ttacaggccg ttgtagcagt
     7801 gcttcctaga gatacaagtg cactacgtac ttgtgctggg gatgcagtcg gatgtaaaga
     7861 atggtaaagc gcagcagcgc cggtcacgaa cggcgcggcc acactcgtcc cgcttaatgt
     7921 ggcgtaagag ctgccaagat acgtagtatg tatattcacg cccggagcgg cgatcgttac
     7981 cttacttcca tagttgctaa agctagctaa tgaatcgtct ggtccgtatg gagtctgagg
     8041 tcccagtcca ccacacttgc catcgctatc agccatagcg gctacggaga taacgtttgg
     8101 gttgctagca ggagaccata acgacgcatc ctctcctgca tttcctgctg caaccacaaa
     8161 tgtcacacca gcagcgacag aattgttaat agctgtgtca agagcagaag aagtacactt
     8221 gcaaccgaaa ctaaggttag ccgcatctat ttgtccagca ttttgagtca catagtctat
     8281 tccttctata atactagaca tactaccgct accactccta tcaagcactt ttatcgccca
     8341 taatctcgca ccaggagcta taccgacgac gcccacagag ttatcctcgg ccgcgacaat
     8401 gcctgctacg gcagttccat gtccgttatc atcattggct gttgacgtcc cgggaacaaa
     8461 gcttacttgt ttatagacat tcagttctgg gtgggtaaaa tcaataccag tatctattat
     8521 tgcgattcct gcatttacaa cgcgtggatg agaaccattt ataatgctta aaggtccacc
     8581 cacccgggaa tatccagtgg atgctacctg agagaaagct tgtaccttga tgtcgggttc
     8641 tacaaaatct actgagggat ttgataaaat cgaattcaga actagtttgt ttgcaatttt
     8701 aattgcaaat cctttgatag cgttgttata gacaaacgga acaattcccc ccccacttat
     8761 tgcctgctgc gctatcgatt gtgcactttc tgttactcca tttttgagga ctactatata
     8821 ctgatggggt attgtctctg ctaacacctg tctcaatttg gattgggcaa aatgtttctc
     8881 ttgattagca agtttttcag cacttttgag attgcttata ctgtgactaa tactgctacc
     8941 ctgcgtcgta tttgtcgcaa caaatgagtt ttctcctcct attgtaatat tcgaaggaga
     9001 tgctccagtc tggacatttt cttttttact agaagaaacg atattattat ttgctgttct
     9061 tgagtgtttt ttggcttgga cttcaaagtt taacacgttg tagaacgcgg tcgagactat
     9121 atacaacgtt attatgatga ataatgctga aactagtact acagtaattg aatctcccac
     9181 ttgcttcgaa tcgttgtctc gtagttatga tattttaatg tgaatgtcaa atcaagacta
     9241 gatttattat attcacaaaa cactaaagat agtcattgta ctgttcttgt tacttttttt
     9301 agagaacgta aagttggata aaaaaaaggg cctaaggtgg ttttgactta aatcataccg
     9361 tggcagaacc cgtgagagcg cgtgttgtgt cgtttgcgcc tataataaaa tgtggtatag
     9421 agtggttggt caagttatta atattcctcc tcaatttcat tcacgtatgt tttaggaaat
     9481 atttctactc tcgtctacct caacgtacaa tcccataaaa ttttaagaag gctagttttc
     9541 tagttgaggt acatatgata caacacagaa ccatgttggt cccgaagaag aaaccttgaa
     9601 ccagtaacct ggagtctagc cgatagccgt tgtcatagca cagaattgat gtactgggtc
     9661 acagacatca ttattatttg gaacttcctg acttttggcg gtagtagcat ttatgttgtt
     9721 cgttccattg tgtattgatt gctgactttt tcctccagca cctccgctcg ctttattcca
     9781 cccatcctga tatcccttag aatatccatt attgtatgga tttgtatgac cagttggaac
     9841 ggttctatca tatccatgac cgtgacacag tttaaaatca caccctgcat tcttaaaacc
     9901 gtcattatat cctagcttgg aacgagtttg agggctttga tctgcgctta cgttttgcaa
     9961 tacgactgaa caagatccaa tcattaacaa tattgtgcaa acaattacaa taataggtcg
    10021 attttgaacc ccttctagaa acttgatcat actgtagtta caatagtcca agatcatact
    10081 aaacattggc aaacaaaaat atcgcattta tcttaataag atatttgtag aagaatttat
    10141 tctgcctctt ctatttttgt cattatcgtt ctgaaacaat aattactaac ttgtaggctt
    10201 cttgatcgta tgtatcggtc ttcaaaccct gacgagcccg ggttatttga ccctaggtga
    10261 gtgagtgggt aactaatata tcgccgcgaa agaaagaggt gagagtatat atgaatgcaa
    10321 aaaccaccgt tggaacagcc gcagcactag tgttagtctt cctaggggta ggagccatag
    10381 ggagcggatc agccagcctg ttatcaataa agtatccaga caaaaatccg gctgttccag
    10441 caggaagtct aattgcagtc agcgggacgt ctgtcccttc aaacgcaact catacaaatt
    10501 gcaatgtagc agtacagata aaccaacatg gatatatcag ggcatcaccc cagggtccga
    10561 aaggggtaac agactataca aagtggacag caatcagcac aagtcgaatc cagcagggac
    10621 taaaccagat agaggcgcaa cttctatgct atcctccagg aatagcgtca acacccaatc
    10681 tgatgaagca cttggttcat aatgtcacag gagtacaggt agtaggattg cccacaaacg
    10741 cacaatcacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacc gtcgacacct gcggcaccaa
    11041 cgccaaaaaa ggcaccgaca ggacaaggac cacactcgat aataccgcta atgcctccct
    11101 agtagcggct tgagcgaata cggaagttag catctacagg tataggaaac cgacaggcgt
    11161 catttagtct agtcccacta gttgacagag acgaatatct ttctttgaat acaggtgtgt
    11221 ctctaactta tgaatctaag cttagccgct taagttcaca gacgtaatac agtgctttgt
    11281 actactttct gacgggaatg ggcaataata atcactacga tagtggccgc atatgaatac
    11341 tctgcagctg acatcgtcgc agctccaata gctgtctcgg ctgacaatct ctacactgta
    11401 tagcctgaca aagccacggg ataccggggc atattcgatt ttaaccgact tgagcaaaaa
    11461 gaattgcctc tatatggcaa tgctctaaca ttaatggcaa agcatagtaa taaaaataat
    11521 gaactcatcc gatttatttt atggcggaaa tcgcaagagt ccaataatcc tatgcacttt
    11581 aacgttatgt gtctacaaat ccatcaacta cgcacccaag aatatctttt ttgtacattt
    11641 agtagcgcct tattctcatc ctacaagtta tgttatagag gtgcgattaa tgatgccgga
    11701 taaaaaaatc ttatatcctg aataagaaat gtaaatggga caattaaaag aacaaataca
    11761 ctattatcca gaaccggttt ttcaatcgct tttacggcag cgctatccct cacgataatg
    11821 atcactagta tgtgcttgat tcagcaaggt gataatgtgg catcagcaac ttcgcaaaat
    11881 actactataa tcacgatcga taatcatact acggcggtgg cacccaatca aaatactgta
    11941 ataggccaat taatttatga agcacatggc aaaatcgtag ctcaaaaagt gataaactcc
    12001 ggaaaaaccg atggagccaa gattcaggtt tcatactctg gtagtggaaa gttcactaat
    12061 ggactagacg ttagagaact atggactttt gttaatacac atagactaaa tggtgtaatc
    12121 caaggtgtag gtcaaggtat tataaagaca atagataata aagaaatcgc tctagcaacg
    12181 ggatacggac gaggttttac tggagtagga gggaagatag tgtatcctac tgcgcagcta
    12241 tacagcacaa actcaactgg aagattagca tttcttaacg aggctgttgg atttagtcag
    12301 tggcaagtag ataactctgg caactatagc tataagatgt gggaattcaa atagaatgtg
    12361 taaacaattt ttttcctttg attatcagta ggaactcggc agggagttag ttggagtagt
    12421 agataaagta taccaactct aatcattgca atagaaatca aaattgttgt tttcattctc
    12481 ttttcttctt ccatatttat actccctttg agcagtgctg cactgggcac ggattctcta
    12541 aaatctgata tcttctcact acaaattagg cgccagatta tataggaaaa aataaaattg
    12601 agtttaacaa aaaattgcaa aaggtgaatg ccaaattaat tcacattcct agaaggctca
    12661 tgctctctaa aagaggtcct gagcgtctga gagaagctcc gtggctgatt attgaatgaa
    12721 acgcactacc tttttaactc acgctcataa ttatatatag agcagcaacg ttgctgcctt
    12781 aagacgaggg gatattattt atataaatta cattacgatt tgtatcatac ccaagctggt
    12841 ttagtgtcaa tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnncta ttgacttctt gaacaaatct ctgaaatatt ttggtatcgt
    13201 tatatgagta tgctttatcg atgttattat gactccactc tctaatagaa tacctgtttg
    13261 caaaagttag agtaccatta tcaaaataag tcctacaatc atgctgactt gactgtttgt
    13321 acccgtcgca gtgcaaaaac atcaaaggag caaatccatt gacagcaaag ttgtaatatt
    13381 ttgcaattgc atttataaca gtagcattat tccattcatt gccatgagtc gtggcgaaaa
    13441 ttgtggcatt aattccgtga tctgctagac atttctttga cccagctact tcataattca
    13501 aatcgttagg agataggttg gtcatccttc tatgagtcat tgtcttggat tctatattct
    13561 gtccatccat ttgcaatgct aatatatcct gccatgtcat tctcgcctta tctgagccta
    13621 cccacagaca tgttataaag aaactaccct taaatccgta tttatctagt attggtttgg
    13681 catttgtaaa ctgactcttg agtgtatcac caaaagttag cattactact ttgctactag
    13741 cactactgtt accagatggc gtaggtgaca actgaatact atatcctatc gccggtaaga
    13801 aagatccggt caaaatgagg gttacgagtg caacaataaa taccaataat gcttggaatc
    13861 tccattcact actactctcc attaaaagtc agtgacggac taaaaacaaa tgttgcttat
    13921 tatgtttcag gtacaactgg ctaaatgtgt cttatgcgca ttaaaatgac taacggatag
    13981 tcattactat tagcctgatc ggagaaatga aaatagtgta tttggagtat gtatcgaaat
    14041 actatttttt aggagtgtga tcgcggagtt ggtgttttcg tctttatttt cgcacgcctg
    14101 actattgcca agaagatgaa tgcaagggca acaaaattca taattgctaa tatcagctcc
    14161 aaggccgatg attcaatatg taatggcgta aaaagatcga gctttgaaac tatcgcatgt
    14221 atagcaaata ttccaaacgc agctgataca agaaggagcc tctttgttaa cagatttctg
    14281 taggctaaca aagacaaagc aaaaagaaac gctgcaaata tcccgcttcc gaagacaaaa
    14341 atatcagcta tgttctgctt gtcatcccca tgcccagtgg catacactgg ctgtatattc
    14401 aataaattag ctaagtaggt gaataataat atgattatta ttgtcattat cttcaacaaa
    14461 agcagttcac cacacccctg tcactaacta aatctccttg tacatcctat cctctatttc
    14521 tctccgctct tcagcttgtt cgtttgtgtt ctcactcgca tcatttttct caccggcacc
    14581 gtctctccct ggtctggatt gagaagcgat atccaaaaaa agttcggcga gtctatttga
    14641 cagtttattc cagactgatg gatggtatag tttaagcaaa gtgatgatgt cttcgacatt
    14701 gccttctaag tagtacttta cgaatctacc ttccttgcag ctatagacca agccaatttc
    14761 gattaagcgt gacatatgcc agttgatagt cggtgcggtg aatcctttat gacgaactat
    14821 ctcactctga gtagctcctg gattttctat aaggtataat attacatccc ttggggtctc
    14881 ctgttggagg atggccaata tattttcata cctctctcct aaaatagaaa atgtatagta
    14941 tgtcttgtat aaccatatat gtcgcgactt tattagacct gatttttcta aaaactttaa
    15001 ttggtattga atatcgccca tagctataag taacttctga ctaatccttc taaggtgcga
    15061 tcctgggttg tctttaatat agctgtatat acgctgcttg ttatctgatg ttggaacaat
    15121 ttgttgttcg tcttgcttgt cgtcatcaat gtacaaccag ctatatctct cctttttact
    15181 ctatatccca tcttctatct atacgtattt gtaggctact gaggtagaat agtaacttgt
    15241 ggtacaccta tctaaatgaa ggtgttggct ggaacgtata ggtgctatta gggatgcgta
    15301 cgtaacagta tattggcctc acagtcttca gtctggaatg cattggatac cactactacg
    15361 attaccaaca cgtatctctg caatagcatt ggtattgctt gtattgattg attgaatgat
    15421 acatagatag atagattatg tgtgtcattt ctctcggtat gtatgcgtcc atatgcttag
    15481 ctattcgtct atcgaagtct ctccttctta cttccactac gtttcacaca agcagaagaa
    15541 gcctgtcaat atagttctaa tctagtagaa ggtagtaata ccaacgttta gagattagct
    15601 cgactttttt ctattggttg gtctcatgag gcaaaaaatc catccatcga atgtatagac
    15661 agatcagata ttttttatat agcaagaaac ataatataga ttatgaatgc aaaacccgat
    15721 ttagtagacc cacgggaaaa agccattact ctgggtgata tagcacctaa atgggcgaaa
    15781 agactagaag aagaaaagaa actgccattt cccttatcca ttagatggtt caagtggtat
    15841 tttgaacttg atattccatc aagatgcatt gttggagaag caaacggatc tagttcatcg
    15901 tacgaaaaag agtgtaacga gtgtaatagt ctcggatggc aatttggaca ctcatttcta
    15961 gtgcgctcaa gatcaggact cgagaaagac gttcacatgt ttcttcaaca ttggaacgaa
    16021 aaacatgtga gatgattgtc ggctatcctt aacagtattc tttattgctc gaataaaagg
    16081 gatagatttc acattatctc atgatgatat tgctatcata tatgaaacta caattccttg
    16141 ctgctatggg aaataatcag tttctgctgg tggatgctca ttaatgcgat tatgaatcat
    16201 ctgaaatgag aagcgctctc caaaaaaatt tctgaggggt catccaagtg caagcgcaac
    16261 tggtcatatt agaatatcaa acgcggtatc acttgtaaat tatatacatt agctttgatt
    16321 tcaatttgga agcagttaca ggtttgctta taaagctatt aggatctatc gctgaataat
    16381 ttgcaaattt ttctttgtct atgttatcat atgctgtcat aaaagcgaat ttcatacgag
    16441 gatttttctc cttcatacgc atatacaatt cgaaaccatt aatcttgggc attttcaagt
    16501 cagttagaac caaatcataa tagttatctg gatggttttg aaatgattgt aaagccaact
    16561 cactatccta ataggcgtct actttgaatt tgttggtact atcattactt tcatatgatt
    16621 ctaggcctat cttgataaca cgtaaaatat ctttttcatt atctacgaac ataattctga
    16681 aaacttgttg ctccgtttgc attattatat ctagattcga tgtattctta tataagaagg
    16741 ataaatacaa attccatatt tggaactaat gacttgttcc aaaagagtac catgatcatt
    16801 aactacctgt ccttccgaat tttatgatgc tctgataccg atggaggaac cttgagaaaa
    16861 ggatgataat taaaaggaga ttgagactgc taatatcgaa tcgccgaaga ttttggatgc
    16921 gttcgtgtac tgatctaaat cagttaatca gattatacgc gaaagcaatg actcatacac
    16981 actactgttt atagaagaaa agtggcatgt tgaagaaaag ttgcctatag ttgatagaat
    17041 tgatataact ggacggcaag aaatcaagtt tatttagggc tatattaaca tcgtataaag
    17101 tagtaaaaaa caaaaggtat atctcgtatc cttccacttt caccttagca ttgcatccag
    17161 atcttcaacc atctttttac tgcttctttc tgaataataa tacctaagcc acagcatcac
    17221 gctaaaaaag accatagata caacatgtat tgctgatcca acatcactct gtagtgctga
    17281 aaatacaacg attccattat catgaagaac gctaaccatg tctcctaata tccaaattag
    17341 tatgaaaatt gacatttgga attgaaaact tctaatattc ttagatcggg ctgctagcca
    17401 tatgaatatg cccaatgctc ccaaaagtag cattattgta atgctgttca taagaaggag
    17461 taacgtacca gggctccata ctacaaccat gttttttatg ctcatattcc tttgcttagc
    17521 tatgccagaa taactgattc caaaaaatat caatactcca acagaaagcg gttcaaagac
    17581 tccgtctgct aataaagtta aaccaaaaaa tccaactact tgatagatag catgtattaa
    17641 aataaaaata gaaagtattg ccgttaattt tttcagattg tcttttaatt ttatagtaag
    17701 ata
//
LOCUS       DAOR01000147           36079 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17975, whole genome shotgun sequence.
ACCESSION   DAOR01000147 DAOR01000000
VERSION     DAOR01000147.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 36079)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36079)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36079
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17975"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1698..1901
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14117..14366
                     /estimated_length=250
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10515 a         7318 c         7611 g        10181 t
ORIGIN      
        1 ttcgatggag cggatcagat cgattctgat tttaatatga taaaaggtgg cggtggcgcg
       61 ttacttaggg agaaagtgtt aatttcagca gcgaataaag tagttattct agcagacgat
      121 accaaatttg tcaagcggtt gacgcaaccg gttccgatag aaattcacaa gtttgctcgc
      181 acctttttgt tgaagaggtt aaaagaaatt ggaggaagcc caaagctacg aacgatggac
      241 aagggatatc catacgtcac cgaaaatgga aacatagtcc ttgatacttc gttcaactca
      301 attggaaacg ttcggaacaa agagatcgaa ttaaagagca ttcctggagt gttagagata
      361 ggattcttta cgagggcaaa catatattac aaggcaaaaa acgatggttc cttcgaaatt
      421 atccaaccaa acagataacg ggcttactgc tatcaacaca ggataaaaat taggccgctg
      481 ttcgaacaaa ctcgccgtac ccgcaagtgt ttttatcaac gtgtccgttc tccatgataa
      541 ctcgacccct tacgatagtg aacacgggcc aaccccgcaa ttcccatcca ttgtagattg
      601 agtagtctga gtacgattga aggatctctg gggtgacctt ctgcaaaaaa tctaaatcga
      661 taattgtcac gtccgcatcc gaccctttct ctatggtacc cttcttcgga tacagaccga
      721 agattcttgc agcattatag ctcgtcactt ctgcaaccct ctccagactg attcttcctt
      781 catttacacc cttacataac aaaactggta acaaagtggc gattcccgga aatcctgctc
      841 ttgccgacca tatgtctcct tgccctcttt ttattaataa tgtattagca acatgatccg
      901 atcccacagt atcaatgatg ccattcatta gtccaaacca catagattgt acgtcaattt
      961 tcgtcctcaa tggaggtaca accttacctg taacatcgaa aaaatcacta gtatgtgtca
     1021 aataatgcgg acaagtctca gtatataaat tcgagttacc tttttctttt tctgttagaa
     1081 ttgaatccaa cgctgttccg gatcctatat ggacaaagta tattgttgag ccaaactccc
     1141 tagcatatgc tcctaccttc gctatagcat tcgcctcaga ggatggcggt cggcattctg
     1201 accataccct taaacttgtt attttcgttc cggtcagttc gcttcggatt tggttttgtt
     1261 tttgaagtct cgagcacatg acctggtcct cggcatgaac aagtacaggc gactgtctca
     1321 cggatgcctc ctggactatg tctgaaataa tttgatcact aatgttcacc tctcgttcca
     1381 ccgtttgatg tgtgccaggt tcaatatcca ttagaatatg attcacatca tgtcctaagt
     1441 tggcgtaaat tttaaacgag ttaattccaa agttcttttt taaatactga atatccttta
     1501 tttgttcagg tcttaggatc gatgcatgaa tcgaataatc aacatgatgt gtgaactcgc
     1561 ttgcccgaag atgcttttca atattttcat acagatcgta caacctgagc attctcatca
     1621 tggtcgtgac gccacctatt gcagcggatt tcgactccgt ttttgctgcc tcttctatgg
     1681 gggtgtatac gccatagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggtgtata cgccatagtg
     1921 aacgtgcggg tctataattc cagggaggac atatttcccc tccgcattta ttttccttga
     1981 agcttggacg ctctgagttg attttgtgag tgccttgatc ttgccgtttt caatcataat
     2041 atttgtttga actattccca ccttcggaat caccactccg gtggcatttg ttatcaaaac
     2101 gtcgcacgtg ttctgcatca ttttagaata tcgagaactg atgcattttc attggattca
     2161 gaggaaacag cacgtacgga ctataaaaag ttaagcagct gactcaggaa tccattttat
     2221 tgagcaacca actgaaggat caaaaggttt ttctatcttt tcgccgttca atattttttt
     2281 tacgttgttt tccattacct gtactttggg atgcatgtca ggctccaatg catcatttat
     2341 tcttccatga aagacgagtt tgagctcaga gtcgaaaagg aacgggtccg gtgtgcaaac
     2401 tgcgccgtag gcctttgcga tattttgagt gtcatctata aggtacggaa aatttagggt
     2461 gttttccttt gagaaggtaa ccattttgtc aaaaccttct ccctcgtaat tgggatcatt
     2521 gctgtttatc ccaaccacct gcaattcttc cgttctgaag atttgttgca ggctaactat
     2581 atcttttatc cgtgctttta cataaggaca atgattacat atgaagacta cgagcagtga
     2641 ctcactctta aaatccttca ttgaatataa tttattgtcg actcctttga gtctgaaatc
     2701 aggcgccttt ccaccaactt ttagtagttg actcgaaact gttctcgcca ttaatgctat
     2761 ttgcatctct ttaagctttt aaaactatcg cagttctttc aatctagaag cgaactatcc
     2821 tgcagctata tcataataac atctttatca catacggggt acatattcag gacgatgtta
     2881 aacaactggc gacgactgtt gcctcgttcc gcgcggaggt cgcggaaacc tatgcaggat
     2941 agttcgctca aagggaatcc gagaggcagc tgggtaaggt gcggctacag caactgttct
     3001 tttgaatggg agtattttgg cggccacaaa tgggccgagt gtcctgtttg tcacagtacg
     3061 acgaaagtag ccaatggaag aagaaactac caacattgaa ggaaatacca tcatcacatc
     3121 ctacatcctt taagatcatg acaccataaa gaacaccttg tcgtgattgt tcatgatggt
     3181 ttaaaggaag ttcgtaagtt tatcatttaa agccagcgaa gtaacattaa gaatttcgaa
     3241 gatggaagtt aagatcttaa ccaaaatttc gcggaaaata agttcatacc acaccataga
     3301 catggagcgg gaatcttgta ggagcaaagg tagtcatccc gttctagtag aagctactgc
     3361 gaagctcgta tcttaagtag aacttactac ctgcataata ccgaagatag catcgacgac
     3421 ttggcgacct tatgctctag ccatcaattt aattaatcaa ccaccaccta cagttccgcc
     3481 aatactaccc cctacatttt cttgatagac atcaattaat tcatttaaaa tgtcatcata
     3541 gttcacatcc ttcttcttgt aatccattac atcttgtaac acagaggtga ggcgcttata
     3601 ggttatttct tctatcctta gagtcctcat tatttacttc aattcagtca aattcggata
     3661 tttactcatt tcgaagatct tgtctgaatg gggtcggccg gatttgaacc gacgatctcc
     3721 agcgcccaag gctggcatca taccaagcta gacaacgacc ccaatccaaa aattatgtta
     3781 tttggcaagg gttctatttc atcaatataa ttttaacttt ctcgacggca atagctctat
     3841 ataccatctt tctcaaaaac gtcatgacgc atgaattttt ttgactccta tcagcgtgcg
     3901 ggaaagatcg cgtctgaagt acgcgaaagg gctcggaaaa aatatcacgt aggaaccact
     3961 gtgtttcgta tctgcgaatc aatagaagct gaaatacgag aaatgggggg tacacccgca
     4021 tttccagtca acgttagcct gaatgacatc gccgctcact atactgcgga acctaatgat
     4081 acactggaag tgaaggatgg tgatattttg aaaatcgata tcggagtaca ggttgacgga
     4141 tatattgccg atacggctgt taccgtgtgc tatgatccga agtccgaagc attggtgaag
     4201 gttgccgaaa tggccttaaa tgaagcagta aaggtggcta aggctgacac caaatctaat
     4261 gagatagggg ccgttatcga gagtactata gttaaacacg gcttcaaacc gatccaaaac
     4321 ttgagcggcc actccttgga acgttatact attcatgctg gtaggtcaat acctaacatt
     4381 aaaaccatcg gtccttcgtt tagcttgtct tccaaccaag cctacgctgt agaacccttc
     4441 gtcacaacta aagatggcca aggcgtagta tacgaaggta aagttcgcaa cattttcagc
     4501 ataacttctc gcaaaccgtc gaagaaccaa gaggccgaca ttttcttaga tcagttgtgg
     4561 aataaattta aaaccttgcc tttcgccatt aggtggatta ctgatttata cgatgaacca
     4621 aaggcccgtc agttgataga agttttagta aggaaaagaa atgtgcattc gtatcccatt
     4681 ctagtagaag gtaatcgaaa gatcgtttct caggcagaac ataccttgat tccatctagc
     4741 aactttactt acgtcacgac gctctgagtg ggcgtttgtt cgctagcttt cggatctctt
     4801 ttctttttct gcaggatatt cgccatatat tgcagtagca atggcaattc cggagcatgc
     4861 cttgcaagtt gaacatgatt tatagatgta gtcgccatca agaaagatcc tcttaatttt
     4921 gtgattgcaa ttttgtctct cttctgtgta aattatagga ctattgacct tcctttttct
     4981 cccaaaaaaa acacttattg tcctaccccc gcggtattgc caacgccgat aactagaatc
     5041 gaatcgcctt ccagcgtttt atcttcgatg actgcggaga cggtattaga taacttatca
     5101 aaagaatcag aaatctcttt tctcatcact gtaatcgcat cttggacaga ctgtttaact
     5161 attattgcat atatcgggat actgaattta gttgcgacat cttctatctg gtacctttcg
     5221 aatctatacc acctatagca gaacctacgc cctcagcgat cgcccctgtc ttttctcctt
     5281 ctagtttcaa agcagcatct atcattatga tagcatctat ctttagacct agttctgcaa
     5341 ccaagcggta aactgcttcg ccaggacgcc cgacgctccc tgtaggccct tctgccttga
     5401 gcatatacaa ttttcttccc agacgaacct cctcgctata taccgtttct agcgattagt
     5461 tttttttctt ttccaagcat cattcttccg actaccatcg gaccaatgcc atctcctatc
     5521 ggtagtccct tcttgaatgc gcttattgcg ctttctaagg cctctgcttc ttgtaacagt
     5581 aaaggcatta ccatttacag ttgtacaagt acgaacatac cagttgtccg ttttcccatc
     5641 aggtaaaagt gcctcacgat cttgtaaatc atatttagag cggtggcggc ctccaaaaat
     5701 tttctcgatg catgaagcat gccattctgt cataccttcc gccatcctct tgatttccga
     5761 tcgtattcgt tcatccttag ttctcatcac atgatcaagt tttcgaatga tgccttgggg
     5821 atcaaggtct attagcatga ttgcaaaata ctcaaggaat ttgtcgattc tctcgcttgg
     5881 gtcagcagca ggcgtaattg aggttttgat gtaatcaatt gcttccgttc tggccgtctc
     5941 tttcatagtt tttgatctgg tcagtgattt tgatacgtca cccaatacca tccatgcctg
     6001 aaatttctgt ccgtatatca ttaatactat aatgggtgca aaccagagaa tatacgctaa
     6061 aggactatta ttggaatcaa aaccgccagg tccgaagaga ccacctaata gtagaaccgg
     6121 aaccgtaagc attgtttata gtctattcca tgctgaatgg attttctgcc caattgaagg
     6181 ttactttgca aatggctttt gctgttcaat aagtaatgcc gctggatggc gttaagcaac
     6241 catcacctaa tttctgaacc gtcgcaagta aaaagtatat gtatttattg ctcagtagag
     6301 ccacaagtta tcatggtagc cggtgcaatt ccaaatttcg attcttttgt atggatttct
     6361 gtggggcttt ttatagtcgg cctgatcggc acaatggttt atgaaaggtc cagttcgaaa
     6421 gcgaaacgtc agcaacctca accacaatca caaagacagg cagcgcatcc aggaacaaat
     6481 actgacattg aatgaaagtg attcaataga acagttatta gtccaaaaat tcaggaaaga
     6541 aggattcgaa tctctcacaa ctatccagca aaaagcctta ccgattgtcg ctaggaggat
     6601 caattgcctt ttagttgcac ctacaggtgc tggcaaaaca gaggcggctg tcatgccagt
     6661 ggtcactatg ttgtcgatta gcgccagact ctcaggcaaa attcgagcaa tctacatcac
     6721 cccgttacgg gcgctgaata acgatgtatt cagaagaatt gtcagatacg ctgaatcgga
     6781 aaatttgcga gtgcaaatta gacacggtga cactacgact aaggataaga aaaaaattgt
     6841 agaatcgccg ccggatatcc tcattactac accggaatcc ctcggtgttg ttctttcaag
     6901 tgagagaatg ctcctagcac tgcagaatct tgagtggata ataatcgatg aggttcatga
     6961 gctcattgct aatgaacgcg gcgcacactt gtctatagga ctagagaggt tacaagcggc
     7021 tagcttagaa cgtgttacca gaattggctt gtcagctaca gttggaaatt tagcggaagc
     7081 cggaaaattc gtttccggaa ctaacagaaa acatgccgtg cttgtcgaca gatcgggtag
     7141 agaatatgat attcgtttgg aatatatcca ggggtcgttt actaacgtgg cgcattttat
     7201 catagaatat atcgtatcaa acaaaataag cgggtctgtt ctgttgttta ccaatacaag
     7261 agatgaagca gagtacctag ctacggtctt aaaaaatcaa agtgaagtga aagtcgatgt
     7321 tcatcatggc tcgttatcca gagaaatgcg gcaggaaaca gaacagagac taagagtagg
     7381 tgatgcgggt gtcatcgtct gtacctcgtc cttagaactt ggattggaca ttggttctat
     7441 agatctcgtg atgcattatg gatcccctcg acaggtttca aagttagtac aaagggtcgg
     7501 ccgtagcagg cacaggcaaa caagccacgc aaagggactg attgtaacaa acaacgctga
     7561 cgatgaatta gaagctttag caataattcg aagaatgaaa aggggttcga ttgaggaaca
     7621 aacgatgcat gtcaacgctc ttgatgtcgt ggcccatcat ctagtgggtt tgtgtctgca
     7681 gaataagggc atgattgagc taccaaaggc atttgatctg ataacgagag catacccgtt
     7741 cagtcacact agcatttatg acttagaaag ttgcgttgag attcttgaca acaatggaat
     7801 aatcaaattt gacagagaag cacgtacgta ccgccggaga attaaggctt atcggtatta
     7861 cttcgataat ctttctatga ttcctcatgt actaaaattc gaagtcatcg atacaataac
     7921 taaacggaag gtaggaacac ttgaccagca atttgtagaa gactacgctg aaaagggaaa
     7981 tgttttcgtt cttaagggtt cccaatggag aattatttcg atcgacgaga aaagaatgct
     8041 agtcaacgtg gaacctctac gtggcgttgc aataaacgtt ccgtactggg tgggcgaaat
     8101 gatcccagta gactacgcaa ctgcacaaga agtgggtact gtacgtcaac atgcaatgag
     8161 aagcatactc aaactttcca atcatgcctt acagaatagt gtaaatgctc taggagtgat
     8221 acccgactcg aataacattg tgatcgaaag cagtattacg aaaaacaccc tcgtcgttca
     8281 ttcaatgttt ggtaccaaag ttaacaacac gatagctact cttctttcta ctatactatc
     8341 ttcacagctc ggctacatag tcgagactag atctgacccc tatagaatca tgctaacgtc
     8401 aaatgccaga attggaaaag cccacataga cagcgtcttt agagattcat atgaatttga
     8461 atctgtcatt attgcttcat ttgcaggtac tcatgtcatg aactggaagg tatgggcagt
     8521 cgcaaagaga ttcggaatga ttaacagaga tgtcttatac gacaagaaag tagcccgtct
     8581 aatatacgaa aggtattcga agacaccgat cagtaaggaa tcaataagag agctaatgca
     8641 cgataaatac gacatcgaaa aaacaacaaa agttttccag agtttacaga ctggatgttg
     8701 ccttatacat tggattgaag taactgactt ctcggacttg gcacaaccaa tacttgaaca
     8761 ccatgcaaaa ttttctgcaa acccactaag cgttgaaaga gggatcattg aactcgtaaa
     8821 agaaagacta gaaaaaacta agcaaaaact gatttgcata cgctgtgcaa agtgggaaag
     8881 aacaatcgat acgctacaaa tacctgaaaa gttattttgt ccgctctgcc gatcgaggtt
     8941 gattaccgca accttctggt ctgataatga gctagcaaag attatttcga agagactaaa
     9001 tggaagtaaa ttacagcctg aagaagagca ccgattcgat cgtgcatgga aagttgcctc
     9061 acttatcaac aattttggca agaaggcgct gatagtattg gcaggttacg gagtaggtgc
     9121 cgataccgcg gctcgcatcc ttcgtaacta tatcgacgat gatggaattt acagaagtgt
     9181 gtatgaggct gagaagcagt atgtgctgac ccgcggtttc tggaacgact aattcagcct
     9241 aggagctagt tatgttacgc ttacgtaatt ttgactagtg aagagtccgg ctttaacgta
     9301 agctccgttt tgccactcat ccctttagtt cctgtcgcgc ccagaactcg tatccggtca
     9361 cctactacca ggttggtcac agaagagctt tgctggcgcc agcctactag tcgagtttca
     9421 cctgtatcgt ctccaattaa tgtgtcgctt acagtaacta attcaccgtt tttagtattt
     9481 acttcggtcg tattcggaga ttgtaagacg atcgcctcta aaatgtaagg agagtctgag
     9541 atttcaacat ctttgatttt tgattcgaac accgacattt ttggaaacga cgcttcgtcc
     9601 tcgattcccc tgatgtatga atcatctttg taaagaagca tagagttccc aaagatacgg
     9661 cttggcacac attctatcat ggagtctgag atgagcggac tgcttagcaa agcactatct
     9721 atggtcagat gtacgaattt accgcttttg tccaccgcga gacaagcaac atttcccttg
     9781 ccagtttctc caccgaacga aactactctc aacgtcatta tttcgatgtc cgttttagtc
     9841 ccgaagaatt caaacgcagt cccttcgtca ccgtgaattt caaagtcgcc ggttccatat
     9901 tgtgggtttc cctctttaat cctaactcca attaaccgaa tctttgcgtt cgtatcaaag
     9961 attttgggaa tcttgctctc gtcaacattc cagattactg tccgcaagtt tcgagttttc
    10021 gtttcgttgg aaagctgcag ttgtagcaac ttcgtcatct ccccacgggg gttatcatac
    10081 tgagaaattc tagggctaga accaacaaag ccggtgatta ccacgttttg tcgtgtttcg
    10141 tgtacatcat cgacctttat cgtaatgaaa tcaatgtctg gaatagaagc gttttcctct
    10201 tctaagactt ctatctgaga actagaagag aggttaatca cgggtctccc gtcaaaacct
    10261 gattttacgt atccgcgaga gatctttact agccccccag ggcggaagcg cgcttcatct
    10321 ggtaagctta tttgatcatc ccataatttg actcgaacgg cagactcgtg atcatagata
    10381 gtcaatgttc gatttctgat ctcttctttg gtatcttttc gaaggaattt gcgcgtagga
    10441 tagatattca tgatccgacc tacaacactc acatcttttg cgcctacaaa tagatccttg
    10501 attgcaccat ctgctcgctt gacattcccc aaagagacac ctacgtcggc agccactaaa
    10561 aataaagcac cttgatctgt tagatatcca gccccaattt tgcgcttctt ctcatcaatc
    10621 aactctctaa cctgctcaag acttatctca ggcttctggc tgagaagcga ctcgatcatg
    10681 gttttaaaat ctgcagacac tgcagttaaa cccccccttt tgtcaagtac ctaaaaacta
    10741 tgtcgagtac ttgcctatat tctttgacag accagtccac actatggatg catttcgcaa
    10801 tagtatgcaa agctgcgaaa atatttctca cttgcataat gatgatggta attgattttg
    10861 ttgaaaacgg caaatagttc attctgttga aaaacatgat aatttttaag gttcgttgtc
    10921 ataatccagt gcatgccatt tgaaaatgaa cacactcttc aaaaattcat cacttcaaaa
    10981 gaagaggaat cttttcatga ccagtatgaa cgggcaattc gagatgtcaa aagtgaattt
    11041 gggaaacagt atcctctgat tatcggtggt acgaaaataa agacggactc tacattcact
    11101 catctctcac ctatcgatac tcgaatagtg ttaggatatt taccacgatc tagtgtcgaa
    11161 gacacacgta atgccatcaa tgccgctaga aactcattta accggtggcg caaacaaaat
    11221 tacaaaaaac gaatagagat ttgcagagca ggagccaatg tattgagccg gtgtaagttt
    11281 gagctcgcgg cgtggttgtc ttatgaaaat ggcaagaaca gatatgaagc gataggagat
    11341 gtcgatgaag ccatcgattt tattagatat tattgtgagt gcatggaaac gaatgaaggg
    11401 tactcaactc taactaggag cgcatatcct gcagaagcca gcagaagcgt tatgaagccg
    11461 tacggcgtat ggggcgtgat tgcaccgttc aattttccag cagcaatatt aattggaatg
    11521 agtattggag ctctgattac aggaaacaca atagtactga aacctgccag cgatgctcca
    11581 attattggat ataaattcgc cgaagtcatg gaggaggctg gtttgccaaa tggtgttctg
    11641 aacctcatca caggacccgg cgctcaacta ggccacgaaa tagttggaaa tgaacacgtg
    11701 gcagggattg tattcaccgg ttcaaaaaaa gtaggatacg agatgatcag gcgatttaac
    11761 aaaaccaaac cccgccctat tattgcagaa ttaggtggga aaaatcctgc aatagttact
    11821 gaaagcgcag atctagacga agcggttcaa ggaatatcta atgctgcgtt tggttattcg
    11881 gggcagaagt gtagtgcatg ctctaaggta tatgttcaaa aaatcatcaa gacagaattt
    11941 gttgagaagc ttgtaaaaag aagcaagagc ctgcaagtag gaaatccact tgaacctagt
    12001 acatttgtcg gtcctttgat aaacgtcggc gcttataaaa attatcaaag gtatacgacc
    12061 ctagcatctc gagacggaaa gttgttggta gggggtcgca ttaatagaag tggtgattta
    12121 aagtacggat tgttcgtaga gccgacaatt gtagacgggt tgccggaagg tcatagatta
    12181 ctcacagatg agcttttcgt gccaatatta tgtataatag tatatgaaaa tcttgaggat
    12241 gcgattcgat tatgtaatag ctcagtatat ggcttgacag cagcaatata cactaggaaa
    12301 aaggaagaga ttgaaacatt tatcgagagt atcgagtcag gtgtagtata tgtcaatcgg
    12361 gctttaagcg caaccacggg agccatggtt ggctgtcagt catttgctgg atggaaggga
    12421 tcaggcacaa caggcatagg cgctggaggg tcttattact taacacagtt tatgcaagaa
    12481 cagagccaaa ccttcgttag caccaagtag gaaagtggta aatgaaaatt tcatataaaa
    12541 ccagaggtat tctcctgatt cccttttaat ttctcgatat aattttcatt gtttcttctc
    12601 atatactaga tccaatttag gatctaattt agcgtgctct aatccgtggc caattttccc
    12661 attaacgagc aacagctact ggaagttatt cttgatgaac actctgaatg acaggtgtac
    12721 acctgccggt agtggatagg cgatataata cggtagacga tataataatg agtaaatgca
    12781 gagcattggg ctgatcaaac cgtgaccctg ctaattcggc cagtgatcag actatcataa
    12841 ttttctattc agatacgatt ccgcgttcct ctgcaataaa tcaacggtct cgtgataagt
    12901 catcccaagt acattcgcag cacaattaac tacgctaacc aagaaagaac tagacataga
    12961 tggaaggtta ttaaagcacc gccggtacct cactgggccg tccgtctcaa caaggaatct
    13021 atttttggga gtatttttca acaagactct cttctcttcc gaataaatta aaactgggcc
    13081 gtacgacacg aacaagccca tgtcggtaga tctagccagt tgcttcttac ttccagcaaa
    13141 ccaatgtagt agaccatttc taatatcata ggtttttagc atttctagta tgtgatctaa
    13201 tgagcctcga gaatgtattg agacaggctt cttggttctc tccgccaggg caagcatgct
    13261 gtgaaagact tctttttgct tgtcgtaaga gctctcgttt atacctgcgt aaactggatc
    13321 gaggcctatc tcaccaatac cgtcgatcga gtcaagatta ttattgaaaa tttcaatgaa
    13381 tctcgtaaca tcttcagttt gtgcaaattc aggatgtatt ccagcaaacg gtatcactat
    13441 gtcgcgggtg gaatcgccga agagctccaa actttttgtc agtgtttccg tatttacggt
    13501 tacagaacac gcattgattc ttaatgcctt cagattatta aaaatatgtt ccatatatcc
    13561 tgagtattcg ttatccgtta gatgaatgtg tgcgtcaact actgtcatcg agtcgtacag
    13621 caagcaaaga aaggttttga taatattgag cttactcgca tatgtgctac tgcagcgtta
    13681 atcttagccg tcgtttgact tgttcaaaag ccaagtaaat aaattcaaat cccggcgcct
    13741 cgaaatatgc gatgttgccc gagataacat ccttgaataa gataggtgct tgttttggga
    13801 catttcgttt cggttttgtt aattatgtat atgaggttaa agtttcagga tgttttttga
    13861 tgcgagatgc acgtcttcag actttacagt ttttctacct gcatgaaggg ataactctac
    13921 ggcttgcctt gcgatttttt ctgcgacttc ttccagaact tttcgaagcg cgtctgctgc
    13981 atcgtcactt actctgtcag cgcctgattt cttaatcacg cgatacatac tggcaaggcc
    14041 gagctcaggg ttagacgaca tgatacactt tgacaactgc tcatacatgt taaaaatctt
    14101 gttgttgtcc agaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnaatc ttgttgttgt ccagaaaatc cgatgtggtg
    14401 accgacggga ggaaatctgt gattatattt tacggtaagg cagccttgaa cgaagaacct
    14461 cgcagtgcat agtgttaaac cgtcgaatgc tagctgtgat ccggttaaac atagttagac
    14521 aagagagtca gtcttcacat agacggatat tctcgtattg tgttattcaa taacctcaag
    14581 tgttaaaaat gttaacccta catttcggta aatatgtctt caacttcaaa tggaacgctc
    14641 actatctaat tggactctaa aaaacaagga aaccttgatg atatatttac ggattgaccc
    14701 gaaaaaatga agttttttta tttgactgtg accgatttgg caaggttccg ggggtaatca
    14761 ggatcaacgc tctttctgag agccaaataa tatgcgagaa tctggaacgg aactacctcc
    14821 aaaagaggat aaagcgcatg tcttactgat gggatcctca caaagatgtc atacacatcg
    14881 ttcggttcat ctgaaactcc gattattgta gctcctctcg ccttgatctc gtgcgcgctg
    14941 gctatgttat catcaaaggt cccgtcccga gggttgatta ttactacgat actattttta
    15001 tctattaatg ctagcggacc atgttttaat tcacccgctg cgataccctc ggcatgaata
    15061 tatgcaagtt ctttaatttt gagcgcacct tcaagggcaa tagggtaatg tatagacctt
    15121 cccatgatat agatgtcctt tgcatctttt atgcggtcgg cgactttttc aatttcagat
    15181 tcagaagcaa gcacctcgtt tatagcgtcc gcaagttcta gtttgcctgc ctcgtagtcg
    15241 acacgacctt tgcataacct gtcaactacg ctataaagca gagacaattg tcctgtaaag
    15301 cttttagttg cagccacacc cacttcaggc ccacaatcga tgctgagaaa agaatcgcta
    15361 attctggcta aggaagacgt cggtatatta acaattgaaa gaatctttgc acccaatagt
    15421 tttgcggtct tgaccgagtg caaaacgtct gctgtctctc cactttgaga gatggcaacc
    15481 aaaactgagc tactgtctag agaatcaagt gcgtactgaa attcacttga cataatagat
    15541 tcggctcgaa tttttgcaaa ccttgcaaga atatgctttg caactaaagc tgaatggtag
    15601 ctagttccac ttcctgtgag atagatcgtc ttagaatcaa gtaggatccg gcaaaagcta
    15661 tttattttat ctctattttc ggttgctgct atcataattg tatgtggttg ctcgtgaatt
    15721 tctttcaatg tgtgatgagt aaattttcct ttatctatgg cggctaattc ccaagctacc
    15781 tgcgtaacgg gacgactcac gggatttcca tcagaatcat ataacttcaa actatgtctg
    15841 tcaataacag cgatgtcttt attatcaaga aaaatggctt tatctgtgta cggtaggaat
    15901 ccgagaatat cgcttgagat gaaatacccg ttttctacag ggccgattat aagtggctcg
    15961 tcatatcttg agccacatat tgtcccatct tcgaaagccg ctacgaacgc ataactcccc
    16021 ttcagtcttt tgcatgtttt aattatagtt tgagttccgt ccttttgctc ggcataatac
    16081 ctttcgagaa gatgtgcaat aacctcgctg tctgtgtcgc ttttgaactt atggccaaga
    16141 tgaataagct cttcttttaa ttctttataa ttttcgacaa ttccgttgtg gacgacggca
    16201 atatcagctg tgcatccgta atggggatga gcatttttat ctgtcacacc cccatgagtt
    16261 gcccacctgg tgtgtccgat acctatctgt ccgggcatcg ttatcaagtt aagagcttta
    16321 tcgacatcag ctaccctgcc tatgcctttc cgcgtggaga tcctgccgtt gtctattgta
    16381 gctattccta cactatcgta gcctctgtat tccattcgtt taaggctttc aaccaagaca
    16441 ggggctacat ttagcctacc attatatcct ataattgaac acacaaatgt tagtggctat
    16501 aagagctttt aacaattatg aacgaattga cgtaccattg tgagatttgg catactggta
    16561 aacaggttcg gttaaggtct gtcagttaaa atttctttat accgctaagt atcattatat
    16621 agcggttgtg gcgcatcctg cctgttgttg acagagaaga cgctggtata tctagcgaat
    16681 ggtaaagttc ttggtaaaaa gatcgttgtt ttcgataatg tggaacatat cagagtactc
    16741 tcgaatcctt tagcttggca aataatgcga ttgctgtcgg atactccaat gtatccaata
    16801 gaaatagcaa agaaactcaa aatctacgag caaagtgcat actattatgt acgaaagttg
    16861 gtcgaaattg gggctttgga ggaggccggt acaagccatg tcaggggcgg cactgctagg
    16921 ctttatcatt cttctagccc ggcgtttgga attgaaatga gcgggggcga aaggcaattg
    16981 gatttccaga cgcatgctaa ttatgagcat cgacacgcgc gcaaattttt taacgactat
    17041 attgtgaata attccttcaa ggggttaatc atagtaggtg ctcctgatcc tcacggcccc
    17101 tacaggtcat ccgcaaggga cggacattat gcggtacatc tagctttttt tttaggaaca
    17161 attagtaata ttccctctga gtttatagtg aagttggatg ccgatgccaa ggcagaaaaa
    17221 gtaatagagg aaaacaattt gatatccatt ggtgggccag gtacaaacat aataacagca
    17281 gaattcaaca aatttttgcc aataaaattc aacgagaaga acttctggtc cggtttatta
    17341 gcagggtctt ctgctaagcc ctataacctt gataatcaag gcttaattgc gaaaataaaa
    17401 aatccttaca acgacggaaa aaatattatt gttgttgcag gcgttaggtc gatcggaact
    17461 aaatctgcgg ttatagcgct gaccaactac agtgaagaaa tcctgaagag ttatcagaat
    17521 gaggaggagt gggcattagt ggtgcaaggc tttgacatga atgctgatgg gaagatcgat
    17581 catgtggata tcatttcgga agtaacgacc taagaacgct cctatgacgt aggagctggt
    17641 gcactcgata acgggcctag aacattcaca tgcaacaaaa tcaactgctc ttcttctctt
    17701 ttcactagat accccggcag atttacgatt ctatcaccta tcgacacatg tccgtgaact
    17761 accacctgcc tcgcttgatg cggtgatttt gtaccggact tcttcatgac tatggtttga
    17821 agtctcctct ctaacagatc ttcaatcttg agatttagga tgtcgtcgag ggaggcagat
    17881 tcattcacta ggcctagtct gttcaaagac tttagcaatc ggttttctct ttcagttctt
    17941 gcttcagtag ccaaagaaag taatgctcga gcttgatttc taaatctagc tacttcagtt
    18001 tgtgctttcc agagctctcg cttgttccgc aagccgtatg accctagtgt ataaagctcg
    18061 gcattcagtt gatttgttgt ccagattctt cttggtcgat gatagctctt ttttggttta
    18121 cgaggatcgc ccatacttca gccgccttta tccctacttc ttttcgctct ccttggaggg
    18181 cgtttccttg actttagcag gcgtagcagc agcagaagca gcaggctgag taaccccggc
    18241 agcgggcgcc ggagcgcctg gtggtcctcc tgccgtgggt ctggccaatt tcttgacacc
    18301 cacagcaccg ccttttctgc ctgtggtcct tgtgcattga cctctaactc ttaaaccaaa
    18361 catatgtcta taaccgcgcc aactcatgac agatttttca cgttctatat catttgaaat
    18421 cataaaatcc aagtctgaag taataagatg ccggttagag ccggtctcca tgtcttttcg
    18481 tctgttaagg taccaatctg gcatacccaa tcttgccgga tccctaatag ctgactcgat
    18541 gtcggctaat tctctatctg ttagaaatcc cacgcgaata ttaggattta ttttcaatga
    18601 ctgtagtaag acctgtgcga gattatgacc gattcctttg actatgctta aagcgacaat
    18661 tatcttcctg ctcccgtcta tgtcctttcc tgcaattctg aggatatgct tgtattcagc
    18721 ggctgacaat caaaatgacg aaagtatgtc ttaaataaaa accatactag attagttgtt
    18781 ggtattcatt ttgaccctct gaaggtttcg caatattgcg ccttgccagc ctgtcaatag
    18841 atgttttaca acgatacata tatgactaag agaaactatg tgtcgattaa tattggcatg
    18901 ttgcgccttc gtccttttct taccagctca acatgtgcct taaaacgcga gggtggctga
    18961 tttgctatgc gcctcgtaat gaaattcgga ggaaccggag tcaattctgc caacaaagta
    19021 aaagaaattg ccaacctgat aaaagcgcag caccgcgact cgacaaatga tgttgtcgta
    19081 gtcgtttccg ccattcgtgg aatgactgat gacatattct caattactga caacataaaa
    19141 gacgggcaga aggtttcagt cgaaaatttc atgaaaaagg ttcgcccctt tcatctagat
    19201 attctgcgaa atgctatcac caatgagggg attatgaaaa aggccgagtt agttgtcaca
    19261 caacttcttg atgagctaga gaacgttctg gcaggtatag tgcttctggc tgaagtaacc
    19321 ccgaagtcat tggattattt gttgtctttc ggagagcgtc tctcgactcc cattgtttcc
    19381 tatacactac aagacattgg cataaaatcc gaacatctaa ctggtagaga agccggaatt
    19441 cttactgact ccaattttgg agaagcaagg cctttgatgg atactaccaa gctgcgtgtt
    19501 gctcacaaga ttacacccat gttaaaagat ggcgttgtcc ccgtagtgac gggctttatc
    19561 ggagcggatc aaaatggcat tactacgact atcggtagag gtggctcaga ttacacagcc
    19621 accataatag ccgccagtat taaggctgac gaagtatggt tgtggagcga cgtcaacggc
    19681 ttaatgactg ccgatcctaa aattgtcaaa gatgctcaag ttcttaaaga agtatcgttc
    19741 gccgaggcta tggaaatggc tctatttggt gctaagtata tccatccacg ttctttctat
    19801 cctgtgatcg attccaatat tccaatccgt gtacgtaatg cctttaacgt caaccatcca
    19861 ggtacaatta ttacacaaag gccagcaaaa gcctcacaaa aaattgtaaa gtcaatcagc
    19921 gccatacgtc atacagcctt aatagatgtt agcggcgggg atatggctgg atctcctgga
    19981 actgcttcac gtatcttcga tatccttgca aaaaatagag taaacataat gatgatctct
    20041 cagagccctt ctgaatctag tatctctatg gttgtgagaa aaagcgatct agataaggca
    20101 acgacggcac ttgaacttaa tttactggga aagataatca agaaggtaaa cgtcaacgat
    20161 gacgtcgccg tcattgctgt agtcggatcg ggtatgaggg gaataaaagg agtagccgca
    20221 aaaatattcg gatcagttgc acgcagaaat gtgaatgtta taatgattgc tcaaggttca
    20281 tctgagctta acctggcgtt cgtcgtcaat gatgatgcct gcgaagtggt agtcagagcg
    20341 ctccatactg aatttaaact agtatcatta agtgccagac acaaaaacat cccgagaaaa
    20401 ggataaatta taatttagtg catgtggaag aaatgacggc acgcaaaggt aaattatacg
    20461 ttgttggagt cggcccaggc aatcatgacc acatgaccca aagggcaaag aaggtgattg
    20521 aagaaagtga aattatagtt ggctatgata cttatgtttc tcttgtagaa gatttgatcg
    20581 agggtaaaga agtctatcgg tatgctatga cgcaggaagt ggaacgggcc aaccaagcga
    20641 tagattttgc agaagatggg aaggtcgtag cattggtttc ttccggagat cccggcatct
    20701 atggtatgat aggtttgatt tatgaaattc tctccgaaaa aggatggaat agagacactg
    20761 gcctctatgt tgaatgtgtg cccggggtct cgtctttaaa ttcttgcgcc gctttagttg
    20821 gttctccatt aatgactgac tttgctgtgg tcagcatgag tgatcttctg gttccttggg
    20881 acataattgt caaacgtgtt gaggcagccg cggccggaga ctatgttact gtgatttaca
    20941 atcccgctag caagaaacgc attcatcagt taaaagacac tagagatatc tttttgaaat
    21001 acagaaaacc ggaaacgcca gttgcaatag ttaaaggtgc gtacagagaa agccagacta
    21061 tcgctattac tacgcttgaa aacatgctcc aacactcaga aatcctcggc atgattacca
    21121 ccgttatcgt cgggaactcg tctacgtttg actataacgg catgatgata aatccaagag
    21181 gatatagatc gaagtacgac ttggtcaaaa aggacggcgg acaagaaagt cctacttatg
    21241 caggaataca acagtaatac tggtaagagt tataatagta gtagaactag tagtatcgag
    21301 aattgaccaa agaccgactc ctctagtgag agattgtttc gtcaaaattt ggttctcttc
    21361 ccttgaaatg tttgcggaca ggttcgatta tttggttgag atatttggcc gtagcatctt
    21421 ttaaatccat tgcatggatc tttttgtcaa cgtagtctct ctctagctcc ttaaaagttg
    21481 catacgaaac acttccacca tatttttgtg gcctttcgat cacaatttca ttattttcat
    21541 gaaatacaac atatctaatt aactcaagta ttggattatt ttcagccgta cctagtgggc
    21601 aaaatccttt cataattttt tctctgatgg tttcctcgtc atcatggatt aggatcccac
    21661 ttgcggggtt gctcttgctc attttactaa acgtttctgc atcacctgaa ccggcctctg
    21721 atgattctgt ggtgagatgt tctgaaagcc ccggaagtag atgatgatgt accgatactg
    21781 gaactttcca acctaattta ggaaatattt cccttacaag catatggatt tttctttgat
    21841 ccataccggc gtgaacaatg tccagctcca atgccttgat gtcaacggat tgcataggag
    21901 gatacagcag ttgtgcaaga tccaggttct ctcgacctgt tctgcccata atggttaggg
    21961 atctgattgt tctaggcaga ctaatgtgtt tactaaaacg tactatatct ttccagtaat
    22021 cattcgtcct gtgatagagc tcgctaccta actctattga tatgcctgga caaaaatatc
    22081 ggaacgcatc agaatagtat tccgaaatct ccttgatttt tgcccaattg ccgcggagct
    22141 tgtcattgat ataggtatgc caatcagcca aaaagacagt gcattgaact tgagctttga
    22201 taaaatcatt gattttaaaa ccggttagaa tcaaactacc aagatgtaac attccagaaa
    22261 tttccaagcc gatatagtgc tttggacgag ccacggtttc taaaagggtc ctcaattcag
    22321 gttcggtaac cacctcctct gtcggatccc tcgagatcaa aaatagccga tcttctatat
    22381 ccattataga attcgggcgt taaattaagc ctattagttt ttgcattgcc atataggtga
    22441 tgaattgtca actagtatta tggtatggat tgaacgctgc ctagtaatat tatgataaca
    22501 ttaatggttg gaccgttctt tctttctttt tttcttctat gtacgagaat atcggcgcgc
    22561 ccaaacttct ataacctgcg tgtgacaata gggtttcgat aatagtgtcc ctgcatgata
    22621 ttgaaagggt ctccatcact cttttgatgg ataatattac tgacaggcta ttgaccagct
    22681 ctgctcaagc tgaaaggcct cccatggtca aagaagaaaa gtttttgccg ccgcctattg
    22741 cagaacacgg tttttctgcg gtgattgaaa ttcactatca cgatcacaaa atgaaaaaga
    22801 aaatatcgaa caggtttcta ttcgataccg gtgtaagtga aaacgggata atctttaatg
    22861 ctgacttatt cggagtcgat ttaaccaata tcgaggcgat catcctaagc cacggtcacc
    22921 tggatcattt tgcaggtttg ttaagtgtca tgaagcggat ctcaagaccg ctcaaagtag
    22981 ttgcccatcc tgacgcgttt ttaaaacgtt ggctaatatt tcctgacgga acgaaagcaa
    23041 agatgcccgt actagatgaa atagaactga agtcgcacgg cgcgctgata gaaaaaagaa
    23101 ggaatgccag ttacttgcct ctcgataatc atgctagtga tgacaagtat aatgattatc
    23161 gacatcgatt gcttgtcact ggagaaattc ctaggatgac aacttttgag aaaggctttc
    23221 cactacagta cctggaaatt cccgaaagaa atgaacttgt tcctgaccct cttgtaaacg
    23281 atgaccaagc cataattgta aatattaagg aaaaagggct agtaatcatc actggctgcg
    23341 ctcacgcggg aataattaac acgatcaaat atgcgaaaga tatcacaggt gtaaacaaaa
    23401 tatatgcaat tataggtggc tttcatctca ctgggagcct ctatgagggt tccatagagc
    23461 agaccgtaca agaaatacaa agtgagaagc cgaattatat cgtaccgtgt cattgtacag
    23521 ggtggaaagc agttaacaga attatccaga cgatgccgga agagtttctt cagagtagcg
    23581 ttggtacaat ttttaatttt taattcgtaa ttcagctctt cttggtctat gcagtctttc
    23641 tgtttggccc cgattgttct gatgctctct cagttgtgat gataaattat ttaaaatatc
    23701 tttctgactg taccagtgga taggctcata acagcttaat ggcgttgttg taataaaaga
    23761 tgagaagagg caaattccat agagaccaaa aattttaaaa taataataat gggaatttag
    23821 atatgattac cattacgaat tttgagcacc tttatgttgc tagagtcaac aaatttgttg
    23881 caaaaagtta aatccgcagt tataaaaagt taaattgagt tctagaaata tttgattggt
    23941 tgaaaactaa ggctagtgtg gagattgccg ccacgatgcg cgcgaagact gccaataata
    24001 ctactagcgt tgcaactatt cctcctccta gttacggaag agcgaaagtt cggtacgaca
    24061 aaaaggccga atcagaattc tttgtcgaca actctgcact cgctggattt actggagagc
    24121 gaattctgta tatggctgtc agagaactaa ttgaaaacgc gctggattca tgcgaaatac
    24181 accgtatttt accaaatatt tctttgtcct tgaaattatt ggatcaggcg aatgatttgt
    24241 ggctcatttc atgcgaagac aatggtattg ggattccttc ggataaagtg ccggttgctg
    24301 tctgttcttt cttaacctca gctaaatatg tcgagaagca gcaacgagga ctctttggag
    24361 ttggcctaaa gatgattgct gcgttttcta ccaaggatac agaccatcct ctgagaacct
    24421 ggtcgaagtc aagcgaagag ggttcagaat attactatga gctccgaaca gatattggca
    24481 caaataaacc aataatcctc acaaagaaac tgctgagagg cgaagaggtc catattaatg
    24541 gcgaatctgg ttttaagatt gaagcggtat tgcgtgcacg attatctccg attactaaaa
    24601 ataagattta cgattacgtc agccacacta gtattgtaaa tccatacgct tttttggtat
    24661 ttcagacaga tgaaggcaag gtgacctttg atagaagaac cacgataatg cctgaaccag
    24721 caaaggaagt gttgccgcat cctgcagata tggatctgaa aacgctaaaa aaagctatca
    24781 tgaatttcat gcaccaaaag acaacgctcc atggagtgtt gtgtaactcg ttccaaaaac
    24841 tgtctggagg caaggcaaag gaaataatta caaaatcaaa tgtagctcta aaggctgctg
    24901 acaaatacga cgaacatgaa ttaatcaaag tagttaatgt atgcaagcag actagattcc
    24961 atgctccaag tacagaccac ttgagtccaa taggagagga aattctgacg gccgggatga
    25021 cgtctgaata tacgatcgtt acaaacagga atgaaactga ctcacaattg cagacccggt
    25081 tatcgattaa ggtgttgaaa ccgacgctga ctgcatactc gtcccgaact tgtgtagtaa
    25141 acaaccgtcc caccgtggtg gaatgtggga ttgcatacgg cggagacata agttctttta
    25201 aactattcag atttgcaaac aagatcccct tgctatacga cgagggctcg gatgtagcta
    25261 gagaagtagt gtcggacgtg gaaattaaca aaatgggaat atctaagaag gaagtaaaag
    25321 agcaatttgc aagagcagac aatagatctg atagagccgt ggagtttctg ccgatacata
    25381 ttttcttcca tatctgctca actaaaattc cttacaaaac ggctggcaaa gagagcatcg
    25441 cctctgaggg tgatttgaag aaatacatga aggcctgttt agcagatctg tacagaaaag
    25501 taagcgccca aattcgcaag gaattgcgta tgaaagaggc agaaagccga ttgcgacttt
    25561 acaaacatta tatacctttg atcatatccg cgatctccga atctatcatc gtcgataccc
    25621 agaagctctc tgaggctttc tctcaacttg cggagaagca tgtgaggggc gagattactg
    25681 gtgtagctgt ttctgaaaaa catgataaaa tcacaggccg gaggattgaa gaagaggcag
    25741 acaagtctag cctcttgttg aatggaaaaa gacctcaacg tacctccaaa tctgctcgta
    25801 ctcaagagaa tatgaaagtc cagggtaaca ggactgcagc aaaaaagaat atcaagaatt
    25861 taaggggtta tcagaaaaaa gaccagacaa cgatggacgt tttctaccga ccagatggaa
    25921 ggagaaaaag gtaattggct gtcaaagata aacgtcatca agaagttata aagaaaataa
    25981 aactactcat gagcgatgtc gctgaagatc tgatggtgga aagaataatg cctgttttag
    26041 atgtacccaa ggtaggatat gacaatacag tttggtcaga acagaagaga atgcttacaa
    26101 tcggcggcaa gacagttcaa gtaagtcctg atagttcaaa gaaaattccg acattctctc
    26161 aatatctagt catggccaac gccatcagaa agttgcttga cgaagaaatg gaaagtacga
    26221 tcaggggaat gtattatgct acactcactt cggtaggaga tgaaaatgct agacaaaaat
    26281 tttggaacag tcaggaaaat tctgatgacg caattaaagc tatagaatta ttgacagctg
    26341 ttccccgcga agaattttct gttacctcga agccaaaagg gatgatatct ggtcctatta
    26401 ccctgcgagt gggcggtgat attatcgatt gtaatcttgg aagtcgggcg acatcccagt
    26461 taatcccgac gaatatacgt gacgtagaaa tagtcaatgt aaaggccgac tttgtaatgg
    26521 tcgttgaaaa ggataccgtt ttgaacaata ttcgaaagtc aggatttatt caaaagtata
    26581 acacaattct aatgactggc tctggagaac ccgaccgtgc tacgagaata atggtaaaaa
    26641 ctctgaatga agagtggaaa aagcctgtag ttatttttgc tgacgctgac ccgtggggac
    26701 taggtattgc tttgcgttac aaaatcggca gcgagtctct aagctatgat agcgatagac
    26761 tagtatctcc agacgctaaa gtattgggca tgatgttctc tgacatttac gaatataaca
    26821 ttccggaagt tgcaaggctg gatgcctcgc aggaagatat caacagagca aatgatatga
    26881 agaagaagcc ttggctaaag gataagcaat ggcaaaaaga gctaaacctc ttcctacaac
    26941 gtagggaaaa atgcgagctg gatgcattct tcaaacacgg gttcagattt ctagcagaga
    27001 catacttacc tcaaaaactc cgtacggcag ggttaatctg aaagcgatct tctacgtgca
    27061 tacaaatgtt ttaggtaata tcctacctca gtaccttcct atgtacctaa tttgtcttgt
    27121 tggctcagca tttttggttg agtttgatgt ggagccgatg gtggtagata tctagatagc
    27181 tcctggagat ccatcactac cccgtaatag cccctcacat tttcaagagt tgactcgaaa
    27241 tgtttgctgt tactcgatgc cgtggcatca gaaataagca cgacgtcata cccaatatta
    27301 aatgcgtctc ttagagatgt ctctacacaa atggatgtat caatcccaca gaaaataagc
    27361 gtgtcaattc ctaggcttct tagccatacc ccaatctcag tatcatgaaa agctgaatct
    27421 cttcttttta tgactatgtg atcgtcgctg agtggcttta tatcgtcaac aatttctgca
    27481 tcccaggtcc ctcggacgca gatcggtttt ttcatgatcc tttcttctcg tgccttcggc
    27541 aggatcttgt gtgttctagt caatagatca atcccagaag actccctgac agcttgggta
    27601 taaaaaaccg gtatcgcagc atttcggcat ttagtgatta gctcccgtat cttcggaatg
    27661 acgcgttcat attgcgaagt gtccattccc agaagatcgt aagatccacc cttgcttgca
    27721 aaaccatttt gaacgtctat aactattaaa gcagggttga ttttgtattc ccttagcttt
    27781 atcatgatcc ctacgtgtat cttgtttgga gactctttat aataccataa gaaagcaaaa
    27841 tgtggagata gacttaaaca atatttactt caagttaccg atttacgaac gcctctaggt
    27901 tgaaggtaaa atagactact agtacgtata tataattatc tagccattat aaaatactgt
    27961 gctacgcaac gatgttcttg gcaaggaggc tacgctatac acaaggctgc cttctggcag
    28021 actaagatgc accgcatgtg ctaggttttg tgaaataccg gagggtaaag ttggcctctg
    28081 cggcatcagg ggagtagtag atagcaagtt acgattattt gtgtatggaa gggttattgc
    28141 tgggcacatt gatcctatag agaaaaaacc ggtgacgcat tacaggccag gttcctcaat
    28201 tttttctatt gccacgactg ggtgtaattg gctttgcaag tactgtcaaa attacgatat
    28261 ttcgcagcgg cgcaagatag agggtaccga aatgacgccg gaacaagtgg tcgataccgc
    28321 agtaaattac ggcgctgaag ggattgccta tacttataat gagccttcga tttttattga
    28381 atttgctaga gattgcggta ttgaagctca taagaagggc atctttaata tctttgtgtc
    28441 caatggctac gataccatcg agtcagttaa catgatgaag gactttttgg attgcatcac
    28501 catagacttc aagggaagcg gtaaacaaga attcgttcgt cagtttatag gaattccgaa
    28561 cgcggaccca atcttccaaa ccctgaagaa tataagagac aagacaacca tccacactga
    28621 aattactgat ctcatagttc ccgaagtcgg cgatgacctg gacgctgcca ggaagctgtg
    28681 cagatttatt tatgacgagt taggcccaga tacgccgatt cattttttgc gattccatcc
    28741 tgattacaag atgatggaat tcagcaatac tccgatcgag acgctagaaa aacatcacag
    28801 cattgccaaa gaaattggtt tgaagtatgc atatgtgggg aatgtccctg gacatccttt
    28861 ggaacacacg tactgtcctg aatgcaaaaa tattgtaatc gagcggtacg gattcagtat
    28921 tggctcgtgg aaccttgatg caaataactg ttgcaaccgg tgcggatatc ctattccaat
    28981 aatagggaaa ccgaataaaa tccataagaa tagattccaa attgtttggt agctctggat
    29041 aagtccaact tattccaact tatttcagtc atttatagag gaaagcagag gtgaaggatg
    29101 acagaaaaag attaaagctt attcagatct ggcatggatg tcaatttatt atgactattt
    29161 tgggagagcc gacttattga tagctaaaac gccgccaatg ccgcatcgta attgaacgga
    29221 agaataattg gagacatcga ttgaaagtgc agtaataact tgacaaattc caagacttgt
    29281 tttatgctat ttttttcagc ttacaaaatg tctaccaaat atctgatttg acccgagcca
    29341 atatccaagg aatagatgtc aagttcgaat tccatcctgc aatgagcttt ggtgagctga
    29401 ggaggagact caatatttca accaatatta ttttaatcta gggtgatggg aactcagagc
    29461 gggccataat gtgaaagtgg catccgataa cgagagaata acgtgcatat gaaccagaat
    29521 atacaacata aatatctgtc gtgatgatta gtaatataga gggaaagaat agatggctcc
    29581 ggaagcaaag tgtccagaat gtgcacgtag agcagatttt gacgatgaca tgaatgaggt
    29641 aaattgtaac cattgcggat ttcatgctct gtatgacgaa tatatcgaaa taatgaaagg
    29701 gagagcggcc gttatggggg atgactatca agcaaattcc gatagacgac ctttttaatt
    29761 ccccgcacat atgagatcaa cattactatc agagtcagtt gtgcgatgag ctggtctacc
    29821 agaaacttcc cttatctgag catcttaact cagcgccaca attctcacat cgaagatggc
    29881 acgcttggat actggccatc ttgaatcgac aacgaggaca ttctacgtca tttgctgaat
    29941 cgccctcgtc gattttggac aacgctctta ctccgtatga cctcttttaa tatcccaagc
    30001 caaaggttaa ttaaatgcgt tccgttcttt ctgatagtaa attggctcac ttcaagcttg
    30061 tcatttcgga tgcaagcgga aagagtgtat cacaagaact ccgggacaag gcagcccagc
    30121 cgctgttggg atcaaggatt ggtgacactc tagattcctc ggtcataggt ttgacccagg
    30181 gaaagatcag gataacaggg ggaagcgaca aatcaggtac gcctatgcgt caagacgttc
    30241 atggaggagt aaagaaatat gtattacttt caaggggtgt agggatgcag aatgccggtg
    30301 aaggcactag gataagaaag ctggttagag ggaacacggt cactgaagaa atctatcaac
    30361 tgaattgcag actactggaa ggaagtttgc aagaaaagac ttcaaagacg acgcagggtg
    30421 gagacgaatc atctgcatca tcgccttctg ctcaagaaga aacattagta aagaaaaaga
    30481 gctagggtag cggtcgttag tttaggtgaa gcgtgttctg catcagcagg ccgtaccact
    30541 gctgatgccg attaatgaca ggattaaatt ttcccgggta attaacctta tatctctcaa
    30601 caaatttagg atccacgttg cactggaagg acacactccc cgattggtat atcaaagaat
    30661 atggatatca acctaatatc aatataggta cggctggtca cgtagaccat ggtaaaacaa
    30721 ctttagtgga agctatcacc ggtgtttgga ctagtgcgca tagcgaagag ttgagaagag
    30781 gtattacaat taaagtcgga tacgcggatg cggcttttta caagtgtcca gagtgtcctc
    30841 cccctgtttg ttactccaca aaccccactt gtccaaactg tggtaaacaa ggtgagctat
    30901 cccgtgttgt aagtttcgtc gactctcctg gtcacgaaag tttgatggca aatatgcttt
    30961 caggctccgc tttaatggat ggagctattc ttgtagttgc agcaaatgaa aaagttcctc
    31021 agccgcaaac tagagagcac ctgctcgctc tgcaagtatt gggcatccag cagatcgtga
    31081 tagttcaaaa caaggcagac ttgactgagt atgaggaagc agcagagaat tataaacaaa
    31141 tcaaaggttt cgtaaatgga actgttgcag aagaggctcc agtaattcct ttatcagcac
    31201 agcacaaact caatatcgac gcgttgatag aagctgtcga aaataatatt aaaacgccac
    31261 caaggaagag agatgccaaa gctgtaatgc atgttttgcg atcgtttgac gttaacaaac
    31321 ccggcattcc aataaaacaa gttaagggtg gagtgattgg tggcgcatta attcagggcg
    31381 aattcaaatt ggaagatgag attcagattc gtcccggttt tttggatgag aaaaaaggca
    31441 aatacgagcc aatcaattcg actatatcaa cacttggaac aggggcaggt cttgttgaaa
    31501 aggtaaagcc aggaggactc gttgctatcg gaacgaaatt ggatccaact ttcgtgaaga
    31561 gtgattccct tattgggtca gtagttggga aaccggatgc tctgcctgat gatgttgatg
    31621 acatctctgt agaagtgcat cttttcgata cagcagttgg cacacaagac atggtcaaag
    31681 ttgaacccat caaggcaaag gaaccactcc gtctaaatat cggaactgct gcgactctgg
    31741 gaacagttgt aagcgttaag gatgttaaga tagaggtaaa actcagaaag cctgtttgca
    31801 tgatgcagcg tagccgagtt gcgattagta gaagaatcac tgatagatgg agattgatag
    31861 gcgcaggcgt agcattatga acgagttccg ttcttcgtcc tcatcctctt actcttttgc
    31921 tttcaattag aaaggcggtt cgtttgttct tcgcagcata gacagacgtg gctgcataat
    31981 gtacctgaaa catgtatgtt tgtatcgaag ttatgtcctg cgtaaagttt attcttcgtc
    32041 tggattatta ttattattat tattattaca attgacatat ctccgcttct catgatcgct
    32101 cgcaagagtt tgtttaaaat atggatagca aaaatatcaa gatgtcgatt catggatgta
    32161 atttgtgaca ctagtttttt aatcgtcctt gtttcggctc cgatacaacg catagacaag
    32221 gttgagagtg agctcggtaa actaaacttg ttggttccag atatcgtgat tgacgagttg
    32281 aaacacttgg agcgaacatc tggtccaaag aaatccatga tggcaaaaac ggcgatcgaa
    32341 atttctagtt cgagaacaaa aatagttcga gttgcaaagt ctcgacacgt agacgatgct
    32401 atagtcaatt atgctattga ccacaactgc gccgctgcaa ccatcgacag gaatttgaag
    32461 aagagacttc tcgcaaacga catcttagta atcacattga gcaacaatat attgagagtc
    32521 atgggtcctg tagttgaaga aaaaatataa aaaattttta agtagagaca tccattagaa
    32581 aagcagcgta tatgatagat tacaacggta tagagattta ttggaccggc catgatggtt
    32641 atcgaatcat cggaactgat gataagaata gaaagaagac agtctacatt gacccattcc
    32701 agctatcaag cgggcatcac aacaaaaatg acgcagacct agttctcatt tctcacaatc
    32761 attttgatca tatgagccta gaggatctga ggcatgttat aaataaacaa actaccatag
    32821 ttgctgcgtg ggaatgtatt gagcaactaa aacttctaga attaaaggag tctaaaggag
    32881 taaaacccga cgataagtta tctatccatg acatcccgct tgaaatcgta tacgcgtata
    32941 acaccaataa aaaatttcat ccgaaagacg atggaaaggt tgggtttgtt tttacaatga
    33001 gcggtcagcg aatgtatcat gcaggcgata cagacattat tcctgaaatg gaatcagtta
    33061 aaccggatat tgcgttcgtg cctgtatctg ggacatatgt tatgacagca aaggaggctg
    33121 cgcaagcagt aaatgaaatt attaagccaa aaagattggt aattcctatg cattatggat
    33181 caatcgttgg caatgaaaaa gatgcagaaa atttcagaga ccttgtttca acgtgtgaga
    33241 caaaaatatt aggacgggat taaaaaagca gcgaataaat tttcagtcat ccaaaaaagg
    33301 tttatctttt ttattttcat tattaaaaaa cttatatacg tacctttttc aatataaatg
    33361 acaatggccg caaagaagaa agcagcaaag tctgcaaaga aagggacaaa gtccaagaaa
    33421 taaagatcat taactgatct ttagtacttg gaaagtacag tgtcaacact gtcactatcc
    33481 ccttcttttc taataacaat atggattagt ttatcatctt attttgtctc attaaaaata
    33541 atattattct atgtatgaga tcaggtcgag gatatcatac gttcgtcact gcgttccgag
    33601 tcatgtcatg atagaacgtt gtgatccact gccatgacgt tgcggaagct gctaaccact
    33661 ctgtcattat gacttacact ccacgcggca agacagtacc ttaaaaaaag ggtgatccaa
    33721 ataacatcca ttaatgcttg aagggggtaa gaccatttta aggatgggca ggtttgaact
    33781 tcggatgaga catctactgg ttattggagt tttgtctctt gcatttacca ctgcttttat
    33841 tatgagatct tatccttcaa aatatggatt ttatcttaat gaattcgatc cttatttcga
    33901 ttatcgcgct acaaaatata ttgtagatca tggtcttaat gcctacttta attggcatga
    33961 caccatgtcg tggtaccctg aagggaggaa tgttccggca acatcacaat ccgggcttca
    34021 catagtctct gctttcctct acaagatatt tggcgctggc acctcacttt tggactttac
    34081 aattctgcta ccagtagttt taggttcttt gactacaata gtcgtgtttg cccttgtcag
    34141 gacgctcgga gggactactg caggtatgtt ttcagcgctt ttatttgcat ttagccctgc
    34201 aataattcaa agaggtaact tgggatggtt caagtcagag cctctgggtc tcttttttgg
    34261 gctattggca gtatatttga tgctttctgc cctcaaacat aaagaagtca agtattctat
    34321 tctgaaagca attgcagggg gattcgtctt gggtctagcg aatgcatcgt gggggggagt
    34381 ccagtatttc agcatcccca tttcaatatt ttttattgct ctgccgttct tcagaaaaga
    34441 taccactatc ccgatgtatg ttgccatagc atttactgtg ataacgatct tgattgcagg
    34501 agcgttccca cgacccggaa tttcgtttgt tgttggctta gcaggtctcg cattaatggg
    34561 tggtacaata tttctgatta tggctcattt tgtcaaaaaa ttgagtgagc ctaggaaggc
    34621 aattcgaaac acactttttc ttttgatagc tttttttgca cttggagttg gattcattgc
    34681 agtgggagca tatcatcatt catcatttag gtaccttaat gcggttaatc catttctgtc
    34741 tactcagaac cagctagtcg catccgtcgc tgaacacttt acacccacgc tggtggacta
    34801 ctttacaaat tactccatcc tgctgatgtt cgctggacta ggcgcttgga ttgcatttaa
    34861 acgtagaaat gatatgtcag tatttgcctt aattttaggc cttacaggaa tctacgtcag
    34921 cgcgacgttt gcaagacttt tagtgtttgc gtcgatcggt attatagtac tagcaggatt
    34981 ggggctctat gagataacgc gtacctatct agctcgagaa tcgtcatcac cttctgcagc
    35041 ctcatcgtca tttgtagcaa cctcaaaatc gaaaaaggta aagaaaaaaa atgttgaggg
    35101 atccaaagtc ttgcctgtaa aaagtcaatg gttgataata gcatatgttg tattgataat
    35161 tgttatgctc tctattccaa tggtctaccc tccgaactcg aactggttga gttctgcaga
    35221 tatcccacca tccattgcca atggtggaac gggctatagg ctgcagacta acgactggat
    35281 agacacatta aattggattg caaaagatac acctaaaaat tccgttattg cggcgtggtg
    35341 ggactatgga tattggataa caactctcgg gaataaaact agtttggctg ataatgcgac
    35401 cataaatcaa acaagaattg cgacgattgc aaaaatgttc attgaccaaa aagagaatgg
    35461 aatcaaaatt gctcatgacc taaaggcaga ctatatcctc gcttacgtcg tagggcagag
    35521 attctctgga gttaacggta ctgcatttta tactttggga agcggcggtg acgaaagcaa
    35581 gaagcaatgg tttatgagaa ttggaggatt tgatgaaacc aagtaccttc aagcggatgg
    35641 gttcactgcc acccccagct tctggaatac gacgcttctt ggccagatta ttccctttac
    35701 tccacaagct tatgctacat ttcaaggagg ccaattgact aatttgcaac ctgactatag
    35761 acctggaaca gttgctcttt atgtaaagca tatcaaattt ccagtcggta gcaatccaga
    35821 taaccagccg ttaagtttag tatattcgtc acctagcttt cagagcaaca acccaggttt
    35881 ggtgtttgga gtattggtat acaaggtcaa tcataactat gtgccgaaaa taacatcgaa
    35941 cccctaccat gaagtatttg tgaacgtcac ccaacaaccg tcacagcaac agctgaagcc
    36001 tttacaaccg ttttccccat cttcttccca tcctattcga tcttcactcc catcttcagg
    36061 tcaaggaacc ccaataact
//
LOCUS       DAOR01000148            5969 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_47126, whole genome shotgun sequence.
ACCESSION   DAOR01000148 DAOR01000000
VERSION     DAOR01000148.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_47126"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1910 a         1099 c         1207 g         1751 t
ORIGIN      
        1 atcaaaaata aatggaaact gatagcgata aggaaaacag ttcaagaaca cctgaatacc
       61 aaatacaatc ttgactcagt tttcctatac catccatttt atccatattc tgttacctcg
      121 tcaaaaactc ataaacagaa aaaaggtgca gtatccattt ctagggaact tatatgttag
      181 gatctgtctt acaatctact atttgtacgt cgtgagttgc atccgatata tcaattccca
      241 agttacaccc cacgagaacc acttgagacc tatgtaatgc caaatacaaa ataacctcct
      301 gaacgtttgt aacatagtat gacacaagaa gcaaatgaaa ggttcagaca attcctcact
      361 gatggaagaa attgggaaag gaaggctact aatatacccg gtgtatttct gttcagacta
      421 ccggcttcta aagcaagacc tgcatcttta gcgatcgaga ttaatccagt tgacacctat
      481 ggctccatca caaagaagag aggagttgtg ataaggtctg cttcagaatt ggatgaaatt
      541 agtcgtcttc tgggtcatcc gaaagtaggt gaactatcaa aaaagataga tgaagtaaat
      601 cctaaaagag aggctactgc aggtaaaggc ggtcctgaga tatttgagat atgagtaagt
      661 aatttttcga actagaaata tattcaggac aaatcaaaaa gcataaagca atcgtaggaa
      721 aatcacaaag cagaaaaagg tggaacatca ttgcgatgcg aacatgggaa aatggaacat
      781 ttgagttcgc atactgaacg gctaacctga atcctgacaa tttgtcatgt ccaatcatgc
      841 tacatatcta gttaggtttt agaaaagatc ttcacgtcta tgatttctgt gccactgtct
      901 tacccttgga gtaataaagt tcggtcagta catcgtgtaa gatttctttt atatcctcct
      961 cttccaaatc cggcacatct gttctttcta ataatttgga acgtttctcg acagatttta
     1021 cttggctttc gagttctctt tccatctgtg acactccgat agtcttaaga agtgtcaact
     1081 ggctctctgc gtttttgtaa aactctttcc tgacttcggc atctcgagat atatttttgg
     1141 ctgagataaa gatcccaaca aaaaggaggt atgcacctag cggtataaat gcctctgtca
     1201 caaggccata gggaggataa acatgatatt gcaatggtgc tatttcgatg gaagcaaata
     1261 ggatcactat gccgatggaa gacataagaa gggatctgcg aattcgttca tcgtgaatca
     1321 gtgaggaggc agtccaaaaa gcaagaccga ataacagcgc cccaacttgc tttgtagcac
     1381 taaaaattag aacatatatt atgctaaaag agactggaga ggacggcaac aaggagaaga
     1441 atacatctcc aaaataactc tggaaaggga agatataata tagcaaaggt atgctcataa
     1501 gtgaaaagta ttttattcta cccattttat gtcgatactg actcagcaat atcgcagttg
     1561 ctatccacat tagtaagaat gaacacacag atagagcatc aaataccgtg gataatgatt
     1621 cactaaatac cacgccacca aaatttgtta catatgaaac tattggatac ggttttacaa
     1681 tgggtaacgg cgcactgtcg agataggatt caagatagat gagcgacacc acaaggttga
     1741 gagagaccag cgataaggat atggtatata gtatgattgt ataatttctt tttgaagtta
     1801 accagcggtt aaacaaaaaa actaggtacg aaagaaatac caacgctgaa agatgactaa
     1861 gatacgtttg aatcttcaac aaaacgagat tatattcatt tagaatacat gtcagatttc
     1921 atctaatcag ggtataatta tacccttgtg gattggatgg tgtgatactg gaagcaacaa
     1981 agtcggaaat ttaacggact gtgcaagacg acagaatctg ggaaatatag gatcagagat
     2041 aaaggttgat ggcattcctg ttgcaaaact agacgtaaga cagtctgtga tcccaggatc
     2101 cggttcactg gattataaga taaattctct tacaaatgtc acgcaatcta catcgaaggt
     2161 gtttactctt acaataccgc ctgacaccca taagccaaat caagtggcag gaacatggca
     2221 ggcggtaagt gacggatggt gggccttttt gaagccacta cctccaggag accatacaat
     2281 cttttacaat attcgcgtaa ctccaacagg tgccctcact tcaccaggta ctaatcctca
     2341 ttttgcagat attacatata ggcttcatgt agttaagtaa gcctcgtaaa ccgttattgt
     2401 gattgtaggg ctcttatatt tgccaaagga ggaatctatt ttttgctcct tacattatgt
     2461 ccatttttgt caacactaaa ataaattaaa tcgaaaatat atttcacatt atttcatctg
     2521 tttagatagc cccacgagtt ttttataatg atgacatatg atagacatta agattggccg
     2581 tagaattgca taatatcgcg ggtttgagtc gtgctgctat aacaggattg aaagggctaa
     2641 taccgaagta ccggtatccg atacaataac gttaaactgt ttaactattt tattacgacc
     2701 aattgccgat gctaaattgc tgccacatct aaacaatttt gttacctgaa atgcgaaaga
     2761 gatcaaaacc tattatcttc actcagttgc ttatagtgac gaaatataat agacataaaa
     2821 attggttgta cgcggacggg ttttgcacca taagagcatg ccgagttata caagtgggca
     2881 atcgaaaaca tttgatcaaa tttctttcta tctgattcat ccaacgaatc cctgagcggt
     2941 ttccattctt tttcttcctt tacacttgct atccgaaatg atggaattgt tctacccatt
     3001 tgcgtactca gcttcttttg ctactgatag tataagtagt aaagactacc ggatagttat
     3061 ccacttgttt cagccattgt tctttttcaa ccctctcgac cttgaaatga cacgagtaac
     3121 aataactaca tctaatacaa atatgcgaag ttctattgtt acagtctata cactccctgt
     3181 attgcgaatt gataccatat gaagtgaggt actcgtgcac gatctagtat tgcatgtaga
     3241 attcgtgtaa ataaagtctg gagtgtctcg aggtgaggga ggggcactat ccaaatttga
     3301 cctttaatct ggcgagtgtg ttatagtagt tgtgtattgc atatttgctg cagagctgtc
     3361 ttccgaaggc taattctgtg attggtgccc tctagcctac tcatcccttc agtccgtcgc
     3421 gccaggctat agcttcgagt tctactctga cagcgtcgtc gacggcaatc acgggtcgag
     3481 cctggaattc aaaggcgggg aaggtcgagc ataccttgtg aacggccaac ggatcgtcgc
     3541 agtcaactag catatggcca ccccattcgc ctactcgcac tacgaacgac tcgatcttga
     3601 aatttgcggg cgctttccat ttgctgaata cctcaagtat ccgcttctgg gcattctcgt
     3661 actccgcagg cgagccttgc gggcgttcga accagctgat catgtacttc attggcatgt
     3721 tcctctattg ttaatgtgtt gcattctatc atcttttctt ggattgtcta ataagggtta
     3781 tcttcagtgc catttgctgc cacgtccggg ttcagaatat tagcattgaa gcaaacaaaa
     3841 attaaaatga ataagacgat ggagagagcg gagactgatc gtttatggcc aataattgaa
     3901 acgttgaatt gggtattaaa tgagatactg gtgttaagta tggagtaata gaccaacgta
     3961 tttcactagg atcacaggag atcgtaaccg tgatacaccg tttcagaatt cactcttgga
     4021 ttaacattaa catgattgta cccagatgaa atatagagga tatatggttc ttatctggaa
     4081 cttttggagg agctgctgtg cgtacgtgta gctgctggaa aagcaatctt attaccattg
     4141 cttaacgcag attgttcttt tctaactaac ccaaacctaa aaactgagtc acagttgctt
     4201 tcttgtgcta aatcggaaaa tgagggagta acaggtttgg atgctactat cgatggagtc
     4261 aaaatacaag gtttgcaaaa ctatcgtgtg cagtcacctt tattcaacct aacaattcca
     4321 gacaataata tcgctggcag caaaccagga accacacaag cagtatctga cggttactgg
     4381 gtattgttga agcctttatc agtcggtagt cacacgatcg agatttatga aagtgatatg
     4441 atagtgcctg tgtcgtttgg tattgaacat agaaaaatgc tttcagatct gcaaatgctc
     4501 ttttcaaata atatggttgc agtaccacct cagtttgatg atttgatttt ggatctgcga
     4561 atagcaagga cttctgctga agggaaagta gagaaaacgg cctcagcaca acttgattta
     4621 tttgacagcc tcagattatg cacgaaacat tatcatttcg agtagtacca ttccttttaa
     4681 cttaatctac taggtatagt tttagatgtt tggatttatc tctcctctat cctaatagag
     4741 gcaagaaagg cgatctagtt ttagatgggt actggtcgaa caaggattag gattagttag
     4801 tgtataaaag caaaggttat gttattccgc aaggatggat aacatagtgt acggtggcga
     4861 gaacataaaa ttacagaagg gagtaaatct tcatggttac tcccacttaa ttgacttacg
     4921 tgattgttat ccatttgtga aatgggctgg cggtaagagc caattattat ctgagttaga
     4981 ctcaatgatt ccttctgaat tcaatcgata ttttgagcca tttcttggtg gtggcgcgat
     5041 gttctttcat ttgatatctg atagaaatat gaggtttagt gcttatcttt ccgatataaa
     5101 cgaggaatta atcacggcct ataaagttgt gaagaataac gtaaaagaat taatcgaata
     5161 tctcaaaagg catcaaagag aatataacag aaatcattct agatattact acaagttaag
     5221 agatgaaatt aagcctgtnn ggtggtgcga tgttctttca tttgatatct gatagaaata
     5281 tgaggtttgg tgcttatctt tccgatataa atgcggaatt aattacggcc tataaggttg
     5341 tgaagaacaa tgtaagagaa ttaatcgaac ttctcaaaag gcatcagaga gaatataaca
     5401 aaaacccctc tgaatattac tacaagttaa gagatgaaat taagcctgtg acagatattg
     5461 agaggacagc cagattcatt gctcttaaca aaacatgttt taatggacta tatagagtaa
     5521 atagaagcgg gatatttaat gttccaatgg gaaagtacaa aaatcctcta atttgtgaca
     5581 ctagaaatct agaaaatatt agtaaagcgc ttagatattc taaagcagtg ataaaagtta
     5641 aagattataa gaatgcatta gttgaagcag aagaagacga ttttatatat ttagaccctc
     5701 cgtatcatcc cacaagttca acagccaact ttaccagtta tacagattat ggatttggtg
     5761 acaatgacca atcagaactt tctaaaatct ttgccaaatt agacgatgcg gagtgcaagg
     5821 ttctgttaag taattcagat acgcctttta tcagaaaatt atattcggac ttttctagtc
     5881 atattaaaga ggtaaatgta tcacgtctta tcaactgcaa ggcttctagg cgtgtaggcc
     5941 ataaagaatt gcttatttcg aactactct
//
LOCUS       DAOR01000149           13376 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_33502, whole genome shotgun sequence.
ACCESSION   DAOR01000149 DAOR01000000
VERSION     DAOR01000149.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_33502"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    8129..8412
                     /estimated_length=284
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4102 a         2401 c         2434 g         4155 t
ORIGIN      
        1 tctattatat cgtatattac cgaactcaag tatatgaacc catgtacata ttggggccag
       61 tgggatttga acccacgacc gccagcgccc caggcttgta cctcactcag tgttgagatg
      121 gtatcctgga ccaagctaga cgatagcccc ccgtattctc agattgactg acctctttta
      181 aatgtacact gattatggaa atatcggagt tgagcaggac ccgcatggat ttccatttct
      241 gcaatcgttc ttgacctcgt atgaaaatcg gagctgaccg attgtttttc atgatacagg
      301 ccatctagga ctatttctcc tttgcaattt ctgattcgtt ctacaaaccc gtctacatag
      361 tatggcatac ttatgcgaaa cgaactggac tattctatgt cccgaagcca ttaatatcaa
      421 gaactgttaa tgagcttaca gaggagcaat tatccagtaa tgcagaggag gcagtcaaag
      481 acaaacttaa ggatcttgct ttgctcatta gagacgaatt caccgcttct tcatttttag
      541 acatgacgga agattgggca aggatatctg acttttctta taaacatgaa attagtgatg
      601 gcgaaagggt caatcgtttt atgatacagc atgatttggg taggaactat gggtttctct
      661 taaaagaaat gtatcggttt gcattagagg atttgttgca taagaaaact gatttcgaaa
      721 tgactgataa cactttagtt gttaccgttg aaattaatac gtcaacaatt gaatgaatag
      781 tgcgaacatg tattctttgt aacatgcata aaatctttca ctagtcaaaa ccaactcaac
      841 aaaatttgtt gtcaagagta attattaaaa gtggaatcaa caccatagaa ggcacctgcc
      901 acaccgattg tactccgtac aaattgggat actctgaagc atggaataca ttaaatggct
      961 ggttaggaaa tcgttaagcg tgcctaaaac ccgaactgtt gctccgcgtg ttgaatctga
     1021 gcaggagtta ggctcatttg tttcacaaat tctgttttaa attgcgagag ctgtgttgga
     1081 ctaaaaggta tcattatctt ttttaagacg tttataaagt caggtgcaaa tataggcgcg
     1141 actatatatc ttgtaaccaa gaactcgtcc tgcgatatca gagcagacaa gaatggaaga
     1201 gtggtcatgg tccggttggt ttacctggaa atccgacttt ctatgcaaca aagcagatga
     1261 cgactcttgc aatcccagca acggagacgc atactacgca gtttacaaag taggatatta
     1321 cacggcatgg catatgttag atactttgaa aaaataggca aactaacaag aagcctgttt
     1381 gcaaacagct agtggcacgc gatccgactt agatctgagt gcagacgcct tacaaaaaga
     1441 gagatcggca atcgcagaaa ttatgaatca caaacaatac tcgagcaagt tatctctctt
     1501 ttcttaagcc aggtaatcta taggattatc tcaggaatgc aaactgaatg tcagatatca
     1561 taacaggaca gtaagttaga gttatcagct cagaaatgcg agaataaaaa catcgactat
     1621 gcgcaactca tgatacaatc gtagaataaa agttattggc ataaatattc gattactatt
     1681 tcaagaaaat atgtcgtatc cacatccgga attgagtcaa tttttttgtg gagttatatc
     1741 gtaacaggaa tgtaaagatg aagttaatac catgaatagc tgttttcctt tggcataaaa
     1801 tgtttctcta cttcatctaa tctttctata gaaccaccat tctctctaag agtggaaaaa
     1861 gatgaatcat tacgaatttt ttgtaaaagc aaaagaactt gttcgtttgt ggctccctgc
     1921 tttatgtcat agcctatttg tctacaaacg tttgatattc taccgtagcg tttattctgt
     1981 cttttttccc agttagtggc actttctgat agtcctgtta caaattttat gactatcttt
     2041 tccatgtgtt taatatgcca ttctctcatc ggaaaactgt tttgcatcaa aaaagatcat
     2101 cacatttcat aatattacgt acgttgaact agaatatatg taagatctct tgaaagtaac
     2161 aatagaatcc tgtcctttat ctaataaaga cagctttcag ccaataatga tggtatttta
     2221 ttcattaaga caaggtactc tcgaatttat gagtgatatg atatttggaa tgaacaaaat
     2281 aaatagatat tctattcatg ttttacacct gactcttcat aatgcctgca tgcagctgag
     2341 aatcacgcgt actagatccg ccattcagct gttagtcatg tggattctat gaagtgaagt
     2401 tcaattaatg cttttacttc ttgaataggc tgcatcaggg ctgttgaatg atattttcaa
     2461 tttatcagat ctaatatgga caattgttta tcaaagatat gatagaaggg ctgtgaactt
     2521 agctataatg tggatgaacc caacttcgta taaatgatgt atttcgcatg atgtggcaat
     2581 gaatgtaact gcaaaacaat gtctatcttt gccattttgg cagtaacgat gacaatatca
     2641 gctacgcttc tgatgacatc taccttagcg gcttccacga tcagcacaca ccttcattac
     2701 agtcatgcta atacaaacaa ctcaacaagt agtattacca acgcgactaa tatctttagt
     2761 gacatcaata gtaccaatag caacgctaac gccatacatc atggatctcg taccagcagt
     2821 cacccacatc atcattcaca tagttcagga acctcaagca catagttcaa ctggatagtg
     2881 ctatcatcgt atcttaaact actgccgcaa aagtttccac tattttttgt cataaatttc
     2941 tttccattac caggcagtgg ttttttgatt tattaatcgg actcatttat tcatagatcc
     3001 tgctcctttg ccattcaagt tcaaagtagg tcattatgac ccaaaagcag ttagtcgaaa
     3061 cgagttacca gctaccgtaa cgtttgttaa aataaatctt gattactacc ccttaaatat
     3121 gtgtcaatca atatattgtc taacatggct caaaacgact ttgttgaaat tgcaaaagta
     3181 aatgaaattc ctgatgggaa aatcaaacat gttgaagttg atggaaagga agtattagtt
     3241 gctaacatag gtggcaagtt ctacgccata agtgacagat gtggtcatat gaacgcctta
     3301 ctttcaatgg gcaatttgac aggaaacact gtaacgtgcc catttcatgg tgcaaagttc
     3361 gacgttatca caggaaagaa gcttactgaa ccaattttaa ctccatctca agggatggaa
     3421 ccattaccac aaagttggca aaaatttttc gaaaatgtgg gtcaacttat ggctcccatt
     3481 aagacatatg atcaaacaac ttatgaaaca agactcgatg gagataggat caaaatcaag
     3541 atttaacaca caccctgcgt tatgggcacg tcacttgtcg tgaggtactt tggccaattg
     3601 aaagcgagaa gtgctgattt gttaagctga gctgtaaaca ctggtttgtg tgcaaacagt
     3661 caaaggttaa ttgcaaagtg aattctacat cggttaccaa ccacgaagaa catgttgcct
     3721 ttatgggctc taattcgctg ttataaggct agtatgaatg ggactcgtct catcttaatg
     3781 tcgcataccc gcgaaatcaa cattaggatc aggtatagac ccagtctcga actttcatta
     3841 agattactgg ttacgggttt ttccaatctt ttgtattgta gtatgttctc ggtgctgcta
     3901 tggatttcaa ttgaagttag gctatggatt gctcctgtta aagatattgt attccaagat
     3961 ttcaatgcat ttagaatctc aatattcctt aagccataca caattcaaaa tgaaagagca
     4021 gacactattc tcgagtaaag tgggttatct aaaggagcga gaaaagaagc atgaagtaaa
     4081 gaagatattg tagagatatt ggacgcttga tgatataaga ctgaagacat acgttatgct
     4141 ttttgcagct ggtagatttg tagcattaga agcattatcc attaaagttc atgatgtaga
     4201 ctttgatgct gatcctgtta aactgtttat tcgaggagac gagggtcata gttttgtata
     4261 tcgggctgac gagtacaagt atcgtactcg tagagtttct gattcagata aggaaaaagt
     4321 gaatttccag cacacagtag gtacaatgat cttaatatcg actctggaag gactttggac
     4381 tgttttggaa aacgttgcaa agaatacagt ggtaataata gcaagcactg gcctagaacg
     4441 tttcatactt tcagtcaaac aaggaggtat ctctaccgag tcttggtgtc tcgagacgtt
     4501 ccgaagatgg tataatatca attagttacg aacaggaata ccagcattat aatattgtcc
     4561 aggtatgagg aataggacta agatatgata ttaaagggat taagaactaa atgaaaatag
     4621 tggacaagaa aatacttgtt gttattgcat ttgcgatagt tgcaacagtt gttgtatatc
     4681 taggattgga atattcgcta ttctttccta cattcttttc tgcaccaata tcaagtgaag
     4741 aagcggtatc cattgtagca catacatcga accttaccaa ttatgatgta aatgatttct
     4801 caactaggta tgtatatatc aaaggtgatg gcagcatttt tggatctaat ataaattcca
     4861 attcaatcgg aaaatatatt ggcaagaccg ccccaacaat aacgacagga aattattttg
     4921 catgggaagt aaaatacaag agcgctgatt atttcctcga tagcactacg ggagaaatta
     4981 tttcaaactc aaacaccaag agattcggtt agaaaattgt tgaaggttaa gttattgtaa
     5041 ttgactaatc tgttctcaga ttagattaat ttttcagtat tatctcatac tgttactatg
     5101 tacttcttgc gaccaaacaa tttttttcca ctccttataa tccaatctat aaccttagat
     5161 ggctataacc gttagagcaa ttataaaatc agcaatagtt aacgaattat cattctctcg
     5221 aaattcttgt gatgctctaa aacactcaca cgtgctactc cttctgccgc agtttctctc
     5281 tttctattga atagtaaagc atctacagtg ctattactcg tctggccatt attgttctca
     5341 tcattgcatt gatgccatcc ttaatcttgt tattacctgt tggaatgtgt gttttctggg
     5401 cattcgagaa tataaacggt aaatttcaac aatataacga agtattctaa gccatatttc
     5461 cagttgataa aagtctattt gtcattgcgt accttggatc tttaacttgg cttatataaa
     5521 atgtagaagg tgtcacttac tgaaattaca atctgtcact cggtgaaatt gtcaaagttt
     5581 tatttcttag cctatttctt tgtggcaaac taccgcgtag aagaaaaagt taagatctac
     5641 tttcaggttc ggttatttta tgatgatgac tggtagatgt tgataatgat gtcgaaccag
     5701 tatctgatcc cgttcctgtt ataccagtat aacttatact accgccacgg atagcagcgc
     5761 ttccatcaga gtattgaaaa taacacctgt ttaccttttc catagagggc ggatccccct
     5821 ttactaaagt tgttttattt cgatagtata ggatataact ccacggtcaa ccaatattcc
     5881 attttttaat ggtacatata cactagcagg cttatctgaa ctaattactt ctgttgtaag
     5941 tgtattaaga ctgtctctct tatcgaatat gttatttgca acacgttgac ttttttccga
     6001 aatatacact aaaatttcgg ctcttttctc ctcaactccc ctattttcca aactgagcct
     6061 aattccaatc agttgatttc tgagggcata ttcaggttcc actttagtga cgacaatcat
     6121 gataagtata tttcactctc tttctctatt gagagaattg atggctccgg atataaaaaa
     6181 catgttacga aaatatagac tcatttgtgt gtagcattag caaagtttga attgtgtgct
     6241 tatgttacct cgtgccttgc caacagcaat ttcctttcct atttcgccag agatacgcat
     6301 ttttcgtcag agaagtaaca tttgtcgcta gtgattcaag agatatcgct agtgagacaa
     6361 tagaatatca ctagtgatac atatataata catattgtcc attgataaat gattcaaagg
     6421 ctcaatgtac aaaaaatctc cttcatatgg cgagctcttt ccgctcttgg aatatctgta
     6481 atagctgatg ccttggactt gattgaggga ccaattctaa gcatcccgcc aataggtgac
     6541 atacctaatg ccattgtaac tggcttatta ttcgcaataa caagaaataa aagatcagct
     6601 gcaataaatt taattaaatt catacctttc ataggcgatt tcattccgac ttattcaata
     6661 actactctta tgtggattta cacggaatcg aataagaaaa gtaaaacgct ccagtatgta
     6721 aaaaattgag tcaatagcct cttttgcttt tgcaaccaga ttaccacggt gtgacagaaa
     6781 cttttatttt tctttctgtt gttcagatga ccatcgacgc gaataaaatt catccttgag
     6841 ttcaagtccc ttaaacattt gaaaatatgc ttgttttatc ttattttgtt tgtcgaattc
     6901 agattgtatc caaagtgttt caaatataga tatgcaagaa gaaaccgtca attcactatt
     6961 tgaatagatg gcagatcctg atgcttcctc aaaggttttc tttgcatcat cgtttacttc
     7021 tatagccaca gaagttgctt gatcaacaac gagagtcacg atcttggtta gtaagggttt
     7081 tgtaatgtat tgaacatgta actgtccatg actttgccta agttcttctt gaatgatgtc
     7141 ctttgcaaca tcgtcacctg cttgtatcaa caccttgaca cttacatctt ttgggacttg
     7201 cgataataag tttaacatac caccatactt cgcccgataa aacgagttag cggtggaaaa
     7261 tagtaaaagg atttcatctc ttgctgacat tagtagattg gtgacaattt ttcttatttc
     7321 tgcaggttca ttaatggtat ctgcgaattc gcttctagtt cctcttccaa tttccttgat
     7381 tttctctttt gcaggagtcg cattattaca aaggttatca aatagatatt gctgtatatc
     7441 tacaaatgat ttggtattag tatggaagag ttgttgtctt tctgtaattt tcccctcatt
     7501 ctctataata taacatagat aattaattcc atctgctatc tgaaaattgc ctttaatttt
     7561 gtcattatgg aatacctctc cgcatttcat catttgtcta caaaaagata tatttccttc
     7621 attgagggca gtaacgaacc tcactcttat tcctttatct ttgagaacct ttatggcatt
     7681 ccataacttt tcattttcta ttaataaagc cactccatct tggtcaatac aacaatcgaa
     7741 tctttcgttt atattcgaaa ttatttctaa tggtaatttt gctatatctt cgttagcata
     7801 ctggatttca atttgagcgg gatgccaatc tgtcggcaaa gtcaaccagt taataatcgt
     7861 tgtcaagata tttaaagtca gtcttgatat tctcgattct cgaaaatttt ttattatata
     7921 tatactgaat gaaaaaagat aattacgatt ttttttggat ggataaatat ttaaaagtga
     7981 gatgtttttt atttgactag ttgcccatat ttctggatgt ccataaggtc cctttcacag
     8041 aagagcattt aaaggagctt tgtaatgctc ctgcagatga gttcggcgtc agtcatgtaa
     8101 atttgttgta taataaggag gcaaatgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnaaagctgg agtaaaatgt gaatggatta cggaagtaaa cctggctaga
     8461 gctgtgccct ctgataaatc ttgaacctcg taagatcaac tcgctctgac atcggaaacg
     8521 ctagccataa cgttttctag ctgaataatg ccgacaggct acagacagac atacgtactg
     8581 gctaacacag tattcgttgc gttatcaaag tcaagacgta ggcacgcctg taaatatgta
     8641 gaatcaataa aagtcaacat acgtagttag aagaagtaat cgtctctaga cattaaaccc
     8701 ttcttcagag aatgcaatat atgtatctcc atatcataaa atgtgatctt ttttggcgaa
     8761 taacagcttt ccgatgaggg aatggcatgt agaacacatg gaaaagacaa ttgtaaagtt
     8821 tgtatcagga ttatcagaga acgcatctag ctggcaacga agacaacata agcgatacgg
     8881 gacaataaca cattgttgca gacaaataaa ctatgatgtg aagcatggcg taacgaatga
     8941 agaagttctc tcatttttac aagaaattcg acttgactcg tcatattcca gtacccaaaa
     9001 taatgtaggc tctattggaa gattagatga actcgagaaa cactatatac cagtcaacga
     9061 aaatgttacc ttttagatca gacaacgcca cacatttatt tgcgtctgac tccagtccta
     9121 ttggggatgt aaattacggt caattattaa gcaaaatctt cgcggtataa tatttagata
     9181 atgctatggc gaccttcaat taaggatagt actgttagca gatacgaccg aaagctaatt
     9241 tccatgtgac atcataagta ggttaggtat attacaatct ttcattgaca tattaatagg
     9301 agctatttgg tctgttttgt tctgtgtctg caatacacat atccatttcc cttttgtcct
     9361 aaaacatcca tgccctatcc ctatactatc aattagcccg actagccgct cctacttcta
     9421 tatttcactt accatataga gagtcaaatc aattgattat gagaagccca atctagtact
     9481 attgtgtcaa tttctattat atgatgctat tttgttgttt tatcgattct gaccatctca
     9541 atgaacaagt aagcaatata ccttagagaa tctatctggt ccaattggaa gtaatgaaat
     9601 cacagatcca ctactcaacg ataaacagat aaaacatggg ctgtaacaat gtaaataaca
     9661 atgaatattg aatccgattt acttctcaga tgtataaagt gtgaacatca aaagattgat
     9721 cacgatagga ctgccacgag tgacaagatg aaatgtttaa tttataaatg tcaatgtaag
     9781 gcctttgttc ctgatagaaa gtactataga tcaactaaaa agtaattcaa aaattcatgt
     9841 gcaaagaaca agataaaaga aatcagcagc ttaaccaatt cggtagctgt aacagatatt
     9901 atccctattt tcattcataa aattagaatc gtgcaccatg cgtttacacg tcttcctgat
     9961 gacaagatca cgtagccaga agtctagatc gataactatg ttgcggaact atttgaagat
    10021 catgcacatc tactgaaaac ccattttgtt ccaattctac tattaaggca agaacacaat
    10081 atcgtcgcac tttattgcta atagtcgcta aaatgtttgg tattgaatgc aactgatata
    10141 ttagattgca gcgctgactt tagtatggca ttacctaaga catgttcatt tagagataaa
    10201 gacgttgatt gtccattgcc tccgtcatat attgtctcaa taggagataa tagtgaagag
    10261 ttcatgattg ctgtggtctg ccaagatcac agagaccaaa tggaaaagcg gttaaaggct
    10321 atgcaatcag caagaaaaat tccacaaggt aaggcaaaat tccaggacat aaaactggtt
    10381 gttacagatt gtatccgtga aatcggcgat aactcttgca ctccacctaa atcgacgtag
    10441 gtattgtcgc cgagagttta tcctgctgat tgatcaaata gatacagaaa atcgtgttgc
    10501 aaacgccgtc ataataaaga tgataataaa ttgtgatatt gcctcatggc aaatacttta
    10561 acatagcgtc tcataagtct ctgaatcgga gacaaacggc ggtttcggag attaggagca
    10621 aacgagagag attgaaacga aaaaatgagg gtgggtattc tcaggattga atatagtggc
    10681 acgtttggca ggtattgtat gtttctccgt ctacatgact aaagtgtgtg ttacatgaca
    10741 gacaagtatg atgcatgtca tcgattccgt cactggcaac aaacctaaag ctttccagat
    10801 gttgattctt acatttgata catcggcaac cgcataaagc catactaatt tttagtcgtt
    10861 agacattgcc taatataaaa tatgattgat acgaccgcat tggcctgcaa atatccgatt
    10921 tagtatttac tattggttta aattagattt tcctgaaatt atgtctatat tgcatatacc
    10981 tgcacgaata ggagaaaaag ctcctaatct ggctgttgcc gaatggatac aaggaagatc
    11041 taccaatctc gacaaagaaa tgggatatgt ggtccttgtt gaagtattcc aagttaattg
    11101 tccaggctgt ttcacaaatg gcattccgca agcaattgac ctgcatatta aatattatca
    11161 tgacggtcta agggtattag gagtggccac ggctttcgaa gactttgaca agaatacatt
    11221 agataacctc aagcttctgc taacttccgg tgaagtaatt ggagaaacaa ggaaggtttt
    11281 agcagagcat gggcgcttgc gaccagggaa taagctaagt tacaaaattc cattcccgat
    11341 aggaatggac acgttgttga agtttggccc attgactcat aacaaggtca tggatttcat
    11401 tgaagctaat tttccagatt tcagatccta tatcgagaag gaaagggagt caatgtacga
    11461 gcaggtaaaa cagtatctaa agctaaaaca attttcagca aggacttttg aggagtatgg
    11521 cctacgtggg acaccgtcag ctatcctaat tgataagaaa ggcgtattgc gacagaccac
    11581 ttttggtcca aatggctttc ttgaaggcgc agttaagcaa ttgttaaacg agtgattgat
    11641 agcataagac catacacgtt tacaagttag gccgattatt cccctacgac catccctctt
    11701 tgttagtatc tctagcgctt ttagcgatcc atgattttcc tttcctagaa aattcgtcat
    11761 ggtttgtcag aatttgtgtg aaagattctg acaatcgttg gtaaggatag atatgctgca
    11821 gctgacagca agtatacttc tattacgacg agagtatact agtcatcata attaaatatg
    11881 atattcttcc agaaatcgaa ggctaaatca gtttgatgca ggctattgaa taggctatct
    11941 tcgtaatcaa atacaaaaaa ggaagaaaga ataagtcaca gtcttcgtta ttgatatccc
    12001 aaaatttcag gtcagctttg gtggattgtt gttataaaca ttagatacta ataatcattt
    12061 tttatgatga tggatggtag atgccggtgt tggtagtatt aatgtgcctg ttcctgttcc
    12121 cccgctgtta cttgcgctgt tgctatgttt atgatggtgg atgctagatg tcggactact
    12181 gcctgttcca ctcgcaccac tattgcttgt gctaccactt ccacctaagg cttgagaata
    12241 acaattgtct actttgttta tagatggcgg gtcctgatgt gttttggata tacaattata
    12301 gaagttgttt atttctttat caaggttaga attaccagta tttacttgtt cttgtactac
    12361 tgaccctccc cctttactta aagccatggc tggggtagtg agggctgtac tagttgttaa
    12421 gagagataat gtgacggcag cagatatgaa taacagtgct ctatttgaat acattgttat
    12481 gctgataacc accctcttgc ttataggatt tgagaatgca acttcgggtt tctaaatact
    12541 gaataattat ctggttatta tgtcgagaat aattaattat cttaatgctc ttgtctttgc
    12601 tttatagtag caacaataac ggactttatt atagatgggc tctactatgt tttgtgcgtg
    12661 accacagata gtttgtggtc tgcctccctg gagacagacc gataacctga caaccaccaa
    12721 ttcaaattcc ttggctagaa atacttcgaa atcctatagc tgagttctta taccatgaat
    12781 gtttctgtat tgaattcgtc ccgtatgacg aaatgccaat taccatctga tacagtgacc
    12841 taaagtttca tcccaacgat aatcatacag tagaaaaaat ttttaggcta tttttgatgc
    12901 aagattttta aaaactaatc ttccttatga gcgcgagagt tgagcctttt cgatcgaatc
    12961 ttaacaaaag taaacgagac aagtataatc aacgaagaat ctcgtttaaa ttgacttaaa
    13021 aatgacgatt atttgactta ttgagcgtga aatagtaaca acaacaagaa gcatgcagct
    13081 gtagactaca gtgactttgt gatgcggaat cgaatgaaaa agcctttgcc acttttctac
    13141 tttggcggag gtaatttctc tgtagacacg gcccccttag aattgcctgc attgtctgta
    13201 attctgctta atcgtctaca cagtattttg aaggaatcca agacatctac ttcaattact
    13261 cctggcagat gttctatatt agggacataa ttgcttatga tataaaggcc ttgtattctg
    13321 acttctattt tgagttcact acttttcaaa tcatcagaat ctgacctatt tataac
//
LOCUS       DAOR01000150           25068 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_1022, whole genome shotgun sequence.
ACCESSION   DAOR01000150 DAOR01000000
VERSION     DAOR01000150.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 25068)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25068)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25068
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_1022"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    16316..16504
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23845..24071
                     /estimated_length=227
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7522 a         4852 c         4826 g         7452 t
ORIGIN      
        1 gtagtagcgg tagtagtagc ggtaattctg gagaaccgca aaaggtaacg gttgatacag
       61 aaacactcgt tgcccaaact caagacagaa tgtggagagc tctgaagagg cgttatcagt
      121 atgactcaaa tttgaagcct gctcaggcct ttctcctaaa tcacataaat tcaaagattc
      181 cactcgtaat tatgtacgta gatttagtgg gttcaaccaa tatgagtatg actttgcctg
      241 ttgataagct agtcagtata atcagagcat ttacgtatga gatgtcctcg gtaatacaaa
      301 gtcataaggg gtacgtgtta aagtacgtag gtgatgcagt aattgccttc ttcccatcaa
      361 gctataacaa attgctcgct tgtgataatg ctatctactg tgcccaatca atgatcacgg
      421 tagtcaggaa tggaatcaac cctatattga accaatatga ctaccctgaa ctcagcataa
      481 agataggtat agatgaaggt gaaaacacaa ttgtccaata tgggcatgac aaaagttctc
      541 caattgatat tctgggttac tgtattagca tagctgcaaa aataacttca atcaccgatc
      601 ctgatagaat tacaatagga gaagacgttt ataacttgat tcatccaacc ctcaaaacga
      661 gatttagaga attaaaggat cgcattcaac attggaagta caccagtaga cagacaggac
      721 agctttacaa actatttttg atggaatact agttccgcca ttgaataaat ttttattttg
      781 tcctacacga aagaataaaa attaatgaaa tcagatcgca tccccgcaaa atcaaaaaac
      841 gttgttgaca tgccagggtg gctcacatgc aacgacatag gaatatagaa cacccctgga
      901 actagctgtt cgtgccaact gttgtgtagg attgaatgcg gatgctaatc acctcggcgt
      961 tactagtaac ctacaatgtg tcgtgctcag atccaccgcg ttgctttcgc aacgaatgct
     1021 atcaaataaa atatcgtgac ggaattgcaa ttggtcaaca ggatggaaga gtcggaatga
     1081 acaacatcgg agcagtggac actactatcg aaaattgtgg aacatcaggt cgggccggct
     1141 atacggcata ctatattcga cgcggcgctg tatctacgca tttatccctt aatagtattc
     1201 aaaacatatt ttagttcagg atcattaaca agtttgttta gaaaacccct gacgatctgt
     1261 ctagtagttc tatctgcagc tccctttcct ctatgttggg tgtttacaac gtaatcataa
     1321 acaaatggtc ttacgtacaa ggagcaaaca tctccaatcc aactaaataa tgtatctgca
     1381 gtcttatccg caatcatttg ttctctttta tcttcaaaca tctgtccaaa tattcgtaca
     1441 ttataaagat acgtcttttt ccctgttttc tgctctattc ctgatagcaa tatagagtcc
     1501 cgctggtcgt ggatcaatcc aaaagataca ttgaaaggca tcttgacatg tcctgacttt
     1561 ggtgcatatt caaacaattc atccattaaa gtcctcaaca aaatgtaaaa gcgttctact
     1621 tgtctctgct tttcatcatc cttattagca ttctttctag taaacaagcc taactagaac
     1681 tattagtgta aactctatta aacgactcta ttgccatgtt gttgctgatc tattacgaac
     1741 accaggtaca ctttagttta tgtgcttctt aacttgttaa ttgctgacag aatctatggg
     1801 aattcaatta atatccttgc gatgatgtgc ccggatatta tttcctcagc caatcgtttg
     1861 tcatcttgca gataatttaa tagaagatca tcagtcgccg ggtcttgtaa taatggctct
     1921 agatgcctca ggatatggtg aatttttatt gtccgacttt tctcctacac tcaatctagt
     1981 tcttttttga tcgccatcat gcgcatatca tgggtcaatt cgtatatgtg cccacttctg
     2041 ctcacacagg aattgacaat tgctcattag acgtgctcta actttagaat actttttatc
     2101 tgagtttgga gttgctatcc taaaatccgt tcgcttaatt tgcattctga atgtttaatg
     2161 catttgaaag acagtattca catttttgaa gattcttcaa agtctagcta caataaacag
     2221 aaaataatga aatttgaaaa ttgcataatg ccctaacata cattccacga aagacgagga
     2281 aaatttacta atgacattct catccaaaaa tcaaagaaga acatgaacat gcagactccg
     2341 atataaaatc aggtgacttg atgagtgcgc tggctcataa attaacagct gattggtact
     2401 catctatgct aattactacg acgcattaga ttcatgacca gaccttatca gcatagagcc
     2461 atggcataaa acgttcgaca atttcgtgca aaaatcggaa acgtaatcct gtagctacag
     2521 actgtcgttt gattagagct agttatctcc tctcttttta atctgcgaaa tttctatgtc
     2581 cgaaatatga tcccgttctc ttcttgccta gtgggagagc tcaattagac tacgtttgtc
     2641 cgatagctaa ccacttgtaa atctatttta acaacacata ttgtgatcaa aatacacaat
     2701 gagagtaact agagcaccgg gccacaagac cgtgtcctat ggaggtaaga atctattatt
     2761 ctgaatatcg ttattactga tacccttcgt agctctacgt tcgataaaat gttccatttt
     2821 tcaaaaatct gaacgaattc ttagtgagtc cgatgaagcc ggcagtacag gttgagaaaa
     2881 tgttataaaa tcaaagctgt aacatcatta tactatgtgg ttgtcaattc tttcgatatg
     2941 gggatgtatg ccagaacttg ctaaagattg tatcgatatg acaaatgctt caagtacgta
     3001 tttgggcata gtcggagggg ccgcgatagg cgcaataata acttggtgga tatataatag
     3061 gcagaagaag acttcacgta agcaagatta tatactgcaa cgaatcgaga atgtcgaaga
     3121 gaagaataga aggattttga cagacttgga agctttcgcg aaccatcacg ataaaatttt
     3181 aaatgaaatt tttctcctaa atcagagcat actcgcattg gagaaaaaga tcgagaagca
     3241 gaaataatac tattcaagcc aacccagttg agcatgcgtt gcattgtttt agtctaatat
     3301 tttcgtttta attttttgag tatcattatc tttctatgcc aaaatataat gctatacaat
     3361 atatttttta ggtatgatca tacgcaagta taagtaatac aaaagaaaga agtttcataa
     3421 tgcagacgac aacaacaaca gctggagaaa aaccaactat acttatgatc aacaaattca
     3481 ccaacaaatg aggacgagat aaatgccaca aaattagatc tagctgctgc agggatttac
     3541 aaatacatcc ataaacaatt agtagaggta aacaattagt agtagttccc aaaagaaaac
     3601 tctataacta ttgctataga gttttatggc attatgtttt atccctaaag atagagatca
     3661 atctttctga taagtatagg cccaatatca tatcaaatct cattgttctt tcaaggtttt
     3721 tgggacataa agtgttcatt gatatgacaa gaaaagatat cttgcgttat ttagagtctt
     3781 agaaagcctg aaaccgttga cccgttacat gaatggggta ggtaacaatg taaagcgtca
     3841 tgtctatgtc ttgcgagagt cgtcataacg caggtcgaca gaagtttggc tattgtaagt
     3901 gggtctttat caatcgctcc aatcaaacat tcagtacggt actcttgtga cttcattaat
     3961 gaagtgaagg aatctgacag catgagctgc aatcagaaca atgttgtcat tatttgttct
     4021 cgaggaatca aggagttgcc accaaaggat gtgaaaacaa actacgatgg atacttgaca
     4081 gtaatttcag gagcgtattc tccgctgttc agtcgcgcgg gattatccga agacatcgag
     4141 aaaatatggc cgacatgata aaccccctaa atgacgagtg atcatatata gacccgaatg
     4201 ttcaccagaa ctgtaacttg agttctgcta agttagggtg tgagatttta taatattgtt
     4261 tacgtactga tgttcacgta ataatcgcgt tttagaccaa tagtaatgcc gtctttcctt
     4321 tgcttctacg tgttaactag ttgcttctac gtgttaaata gttgtcagta caaactttta
     4381 aggaatgtta ttcacgcgca aaatccttgg gctatctggc attgcggcta ttgcgtctat
     4441 gttgttaata ctagtaacag aagagggcgt acacttggca gaatatgtga catctgttgc
     4501 tacaggtctc accttggcca tattaggtgt agcatattgg acgttcaagc cagagattga
     4561 taaggcaatt agggaacgca aacaacagat cccggctggc gcgagtgaaa atagttcact
     4621 cttattagaa aagactaaat cccccgagcc cgaatttgga ggattcttca agaaaggaat
     4681 ccagtatagt acaggaattc aaactggtta ttacatgcga gtcaagaatt cgagtagaga
     4741 aacaaaactt gacgatgtcc atggcgacat taatggaagg ggtataggac atattcactt
     4801 gatctggaat gaagacaaca atcctcgata cctacccatc gatgaaagtg cagatttgat
     4861 gctatttata attccagaat tagatgtgat agtattcatt aaacatgatc caacatctcc
     4921 aaatacgctg aattatagtg atgttccaag accctacgat gaaaataaaa cactttatat
     4981 taaaatttgg tcagggccca caaaattgag tttaactaaa acaatcaaac aaatcagaca
     5041 agaggcagag gacatggatg aataattatc ttagggtcag gttcagaact gaaaagacag
     5101 tctagaggac ttggccatcg cttctcttcg atccctgtat taggcgtatc aataaattaa
     5161 gctatcgggg caagtcacaa acagatgaaa cgatacgaac tggtcaagaa atatggacgc
     5221 gacacaggag gatatcaagg cggagagcac ggtcattagg ctatccggta caaatacatc
     5281 gtgtgtagta aaatctactg aaatgtcttt tggtagatca aactcagacc cggcgcggtc
     5341 aaacacagca gacctaaggg tttactaaat cctatgagca ttgctgaggg gttagacaat
     5401 ccaatggtag aaattgagta caagggtaga caaagtaact ttattcttac cactcaaggc
     5461 gaaggtacat tgaaaatatt cggagaatgg ccacataccc tataacgcca tgggtaaccg
     5521 gcttcggagg atgtgaaaat ctttaagaaa aaaaccgttt gataggtaca aactattaga
     5581 aatatatcgt aggcgactat ccttctatac caaaggataa cttaataccg actctgtact
     5641 gtcgtatttt tcctgttttg ctatctattt tcgcagttat atctttaact tctattccat
     5701 ggattcctcg aattgtcttg gttgcctctt taactgcagt ctgtgctgca tcttcccacc
     5761 ccttctcaga actaccaaca agctctacta ttttggctac tgacatgctt ctaaatatga
     5821 gttacctctt ataataacat agatgttcag atctgttgta gggaacaata tgatacatat
     5881 gcagctgtcg tgcacagaca caaatcacaa aaatcaactt tgcagagtcg ctcgctcaaa
     5941 ctgaagttta cgaagatgct ttttcagcac accaatttcc tataatcccg tcatgccggg
     6001 aaccttaaaa ggaattctat agtccttagt aacagttaag ccgtgatctg gtgtgatagt
     6061 cactgattta ggtgaagaat tttctggtgg cgaaggcaat accttctcac caggagatag
     6121 tgaagaataa ataaagaaat aattagctat tctaccttta tcgtttttcc tttcgcctgc
     6181 tcctttttct taaatgttat ttctagtatt ccattattgt aagcagatct ggctgtttcg
     6241 atatctgttt ctggtggtat ttcaatagtg cgatggtatt tcctttgagg atcttcgctc
     6301 ttgacttcta aagtgccatc atgaacattt attctaatat tgtccttcgg tattccgggc
     6361 atttctacta caactttaac ttccttatca gttgtagtta catccaccaa aggttctatc
     6421 tcaccactaa tctcaggtct actgatacca ccaaatcctc ttgatgattt tacatttcca
     6481 aattctctta cttttggttt accatcaggt ccaatagtca tcgaatatcc atagactatt
     6541 ggtcctacct ctcttacctt tccaccttgt ggtgtgtcat attctcttac caaatctttg
     6601 ggtattcttt tttctatatc ttcaaactct ctttccatct gtcgcctcat ttgatcaaat
     6661 cctctgaaca tatcgtcaaa gaatcctcct cccagaccct ttcttgctcc cccaaagaat
     6721 ctgctgtacc aatcatatgg ttcgatatca tctgaactca attacaatta ttcacctata
     6781 ggaagtatag tgtacctgct acttaagatt ttagatatgc gccttactta tcgtggtata
     6841 acaagcatca tatttaatca gttatgaaag gtgacctagt ctgatattga ttacttacct
     6901 actaagataa tactacttat tttaaataat cctgttcact aatattgcct atggagatga
     6961 ctaaagaaac aacaatgatg actgctcaca ggttgtctaa tgaaataata caatgctcca
     7021 tctattacaa actgagtcat ttacatttga tcacaatgag aaacgagagg tagaattaga
     7081 attcaagcgt agaaacgcac agaactaacc agctgaatat cgatttttcc agattaagac
     7141 acaaatttag tccaaattta aatagacgtc agcatggaaa aaccgaagtg caatagaatt
     7201 cgcgatcaat gttccacgtc aaagagtagt cttaagataa taaacgatat tgaataaggc
     7261 atattgtttt catattgttg attaattcca tctctaggac tacgtcgaga cttgtaaatt
     7321 tggtcgtcgg aaattggagt taattatact gcctccgtgg tgcagttagg actttatcga
     7381 ctgtatcaga gtaatagcgc aatgatggcc acaccggata gccgattcgc aagtcagaaa
     7441 gtgtttctaa ataactcctt tgattgctaa ggcggctttt tttttggata agtttgcgat
     7501 ataagacatc cgcctcaagg aaagatcata tgataaaatt acctgtgtaa tttttttttt
     7561 agtgatatta aaccccttag caacacggtg tcgttgagcc ctgtttcaac gagtaaattt
     7621 tttaatcgat aaacgtgatt tgtactcaag ctttgatacc aactagccta aatccaagta
     7681 tcaaagctat cccaataatg gtgaaacctg ctgctaataa tcctccctgc gtttttggat
     7741 ttacagcttt tcctgatgta tagagcagga atgcgccagc cattccaatg aacaagatca
     7801 ttgtcgtcac catcgtttct ccgatcgttt gaacggacct ggaagggaca atgaatgtac
     7861 tagcacttac atttgctctt tctataatcg aattaaatag acctgatcct agcaggaaga
     7921 atattattat ataagtgaca gcaacgataa tggccagttg gccagtgttt ggcagcatac
     7981 gctaccgtca aaatattttc tataaatagg tttgctaggt gatcgcaaat atttcaaggc
     8041 atttcagaaa ggcctatccg tcaccaaaaa tgacaacacg tctacgataa ataacatact
     8101 tttttcaacc tttttttatt tccattccca atttctgcag tagtgctatc tacctagtct
     8161 cattcgtatc caaaaactcc aactagatta ctaaggaggg caatttcccc tctgaagctg
     8221 tgctgcaaag ggcggaagtt agtcattttc cttgctcatc cgcacccttg cgtttcacca
     8281 gagcgctgag cattcataaa gaaattagtt tatctcaagt caacccttat ctttagcatt
     8341 aagcttgata gcttccttta tccaagaaat tatttgtttt ttattgatat ctttttccga
     8401 atgtatttta aggtggcgca tgccttttcc tgttccttct agtaggtgtt ttgggtctgg
     8461 taacaacgta cccttaaaaa aagccagatt aatataggat attgtttcaa aactgtatac
     8521 aaaggtaagg ttaccccatt ttattctctc cacgacatta ggatgtactt ctaatatgat
     8581 atctctgaga cgcagagcgc gcgctctctt gtggcctgga agcgccgcaa agaaactctc
     8641 aacgaccgat tccatgactg gcagtccgac taataaggct aaatcctata taatttagat
     8701 caaatcagct actacaacta ctctacctac attagagaga aacacgtttt gagagacaca
     8761 aaccttgaga aacattaggc ggagaaaatt caagatttag aacatgacga acggtagccg
     8821 aacgctcata gtgaatcatt ttgaaggctt ttgatacctt atctctataa tagaggttat
     8881 ttagctatgc aatacaaaca cagaccttct ttggcgtcta tcgataagat tgaatacttg
     8941 ctaccttggc ttaatataat tataatagta actataggtt ttatagtctg ttttgcacaa
     9001 cacagtgtat gaggttgatt gataagcaac taaatgttcc agaaaagata ttaaaaatga
     9061 aggattttaa tgatactttt gaattagtga agttagcagt taacagtaaa ttcaatatgc
     9121 acagagcagg attgagcctc attctccaag gcttgcctag tagaatcgga gcatatcata
     9181 tcctaggttc taacgtaatt gtcgtaaatc gatctattct aaacataatc caacagcaca
     9241 agtcaatcga ggaatataat tcctatctct ttatggtact ggtacatgag tatcttcata
     9301 gttttgggat catagacgaa ttaggagtta ggcaaatgac atacgatcta tgttgttcac
     9361 ttcttggcat agaccatcct gcctcaataa tggcgaaggg agatccttca actttgtttc
     9421 cggagcttaa tttgctcagg tgggaaaggt ttgacgagca ttttgaagtc ataaagaatt
     9481 ttgataaaac aactcagtct tatatacagt aataaggttg ctgaatcatt attatcggat
     9541 attgaagaac tgacgaaaca ccggcctggt gaaatcaaaa aaacgaacta aatgaccgtg
     9601 aatactttat gattacaaaa aaagggaaat aggttttcct ttgataaatt acctttttga
     9661 atgactttct tattcgttcg tttgttacat ttcacccatg ccgcccattc ctccaggtgg
     9721 catgccgccc cctcctggtg gtgcaccaga cttcccagag gctattacat cgtctatcct
     9781 gaggatcatt gatgcaactt ctgttgcgga tttgatgatt tgctctttaa cgacaagtgg
     9841 ttcgaatatt tccaatttgt atatatcagc aattttgcca ttccttacgt caattccggt
     9901 ccatttcgaa cccttactct gctttgacct taaatctgtg atggtatcaa ttggatccat
     9961 gcctgcgttt tctgctaaag tcaatggaat cacttctaaa gattcagcaa acttttctgc
    10021 cgctaactgc tcccgaccgg atagtgtgct tacccaatct cttagttttg ctgctgcata
    10081 cgcttctgga gcacccccgc ctgcaactat gaccggtctc tcgagcacgt cttttgtaac
    10141 catcaaggcg tcatggactg atcgctctgc ttcgtcaaca actctttgag atccgcctcg
    10201 aatcagtata gttactgcct tggggtgttt gcagccttct acgaacaccc atttgtccgt
    10261 ttcaatcttt cgctcttcca cgagatcggc agaacctagg tctttagctg taaggtcatc
    10321 aaggttgctc accactcgtg cgccagttgc tcttgccaat ttcgccatat cactttcttt
    10381 gaccctacga accgttaaaa cttcagcctt cgcaaggtag tgctgagcaa tatcatctat
    10441 acccttttgg catacaacaa cattcgcacc ggtcgccgtt atcttgttga ccatattctt
    10501 tagcatttta ttctcttctt ctaggaacag attcatctgg tcaggggaac tgatgtttat
    10561 cttggcatcg aactctgttt tttcaatctc aagagcagag ttaagaagag caatttttgc
    10621 tttttcaacc ttctttggca tccctccgtg gacgacttct ttgtcaagaa caattccctt
    10681 gatcaattta gtgtcatgca gagaaccgcc agcctttttc tccactttaa tatcatcaat
    10741 atctaacttg gatacggaat cacttttttc agaaacctga aggcaggcac taactaccaa
    10801 ctctgctatc tggtctgatt ccctcgagac tagttttgtc tgcattgaag tctttgcgac
    10861 tttaatcaaa tcttctttgt cattttctct aatcttctgt cccatgctgt tgagaatttc
    10921 aattgccttt atagcactct ttctgtagcc gtcaacaata atcgtaggat ggacatcttt
    10981 cacgataagc tcctctgcct tctctatcag ggcacctgct agtattacca cagaggatgt
    11041 gccgtcacct acttcgttat cagttgcctt gctgatctcg accatcatct tggccgcagg
    11101 atgttgaaca tcgatttctt tcaaaatcgt tgcgccatca tttgtgattg ttacgtcacc
    11161 tagagtgtca accagcatct tgtccatgcc tctaggacca agactcgtcc ttattacttc
    11221 agcaatcaat tttgcggcag taatgttatt cttctgagca tctctgcctt ttgtctgagt
    11281 tgcgccttcc ttcaatatta atactggcat tccgccagct gatccttgtt gaattgtagc
    11341 catattcata ttcctcttta aaatttataa attatatggc agagtttggg atattttaaa
    11401 cttactgcaa atttatcgtt gcagccccta ttggtactca aatgaggagc cacaacagat
    11461 aattaagtta gaaaagtatt caaaatgtta ggccttacat ttcttttgag gcagataatc
    11521 aaaaaggaag tggcaggaat aattacttga aaagttcgtc gtattttcct gctgaaactt
    11581 cagagtagat atcttttgca ggtttccctt caattgtcat tccgatgctt tgacaagatc
    11641 cgattacctc ctttaccgca ccctgtatgt cattagcgta ggagctatca attttcattt
    11701 tggcgatttt aatggcagtt tccatagtta tatctccagc atagtttgtt cctgccgtcc
    11761 cggagccttt agttacacct gattctttga ttatcagcgc agctgtgggt ggaactccta
    11821 cttcaacagt gaattgcttt gttgtagtat cgacttctac ttttacagga actttcattc
    11881 cagggaaatc cttggttttt tcattaatgg tatttaccac ttgtaatatg tttactccta
    11941 taggaccgag cgccggtccc agcggaggcc cagaacttgc ctctccacca ttaactaaag
    12001 cagaaacggt ttttttatcg cccatgcttc aaaacacagt tgagctgaaa tataaacgtc
    12061 ttttacagtc acgatagcat atatataaac ggtgaacttt ctgggagcgc ctaatttttc
    12121 atgacgcgta gaatagagtt ttggacaagg aaacagggac ccaagcacta gacacattcg
    12181 ccatatgcac cttcgtggcg atcacaacca tctttctgaa tatatccaat attcagctcg
    12241 ttggtagtac agcattctca tcacgctaaa aagtcaggcc gcgagaacac aaaaaaacac
    12301 gtacaaaatt agtgcgacac ctttttcaga taatttgcat ctaccgttac gggaatctgg
    12361 tatggagaat caagtaacac gactgtcgct tcttgctttt cgtaatcgac tctagttatt
    12421 ttcgctttca tagccttgaa cggcccaccg ataatttcta ctatatcatc gacattcaga
    12481 tctgatacga caggtttttt cacgaggtaa ccttcgatgt ccttgaaggg taattctcca
    12541 cggatttggc ctcgcacatg acggacacct gccagtgcct cataggcgat gtttgaatca
    12601 ggagcttcca cgataatata tcctttaaaa ttctccagaa taagaattga aataattcca
    12661 atctttttcg cgttaattct attttgtata atgtttgcca ctatgttttc ctggccacct
    12721 gttattctga tagcgaaaaa ttttgcgtct aaacctggtc ttgttgccgg ttcctgagta
    12781 ggtgtatcta atgtatcaga tggtgtagcc gatatatcag aaggcttctt aggggattca
    12841 ttatcatcaa aaatatcatc aatttcttca atatctcctt cattctcttt aacgctcatt
    12901 atttttcaat tcaatataga acgggtgtcg gattttataa atttacttcg ctggacaaga
    12961 tcgaatatta taatcagtac ttgtaaccat aagatgataa ttgacttcca cagaagcaga
    13021 cgttgacgtg ctgacactag gagtacattg gatgatgagg ttagagacgc actcaatcga
    13081 gcggcactag ccaatcaata agcgcgtcaa gctcttcaca atctaatgtt atccttattg
    13141 gaccaagtgg cgattcctga tccgcctcaa tcactactac aatgccagta gcagccgcct
    13201 gcttgttcaa aaggaagcta gtgctatctg caactcgatt attaagcaac atacgtctta
    13261 agacgcccat tgcggatcta gacctaatct gttcataaat tgtacgaagt gattcaattt
    13321 caagtgacct accaataacc cttcttccat atcggaactc cggcgaacat tcgatcacat
    13381 tcgctattgc ggcaattact ctctccggcg actctgaggg gttaactgca gcctcaactt
    13441 ttaattcaac gggcgttgtg atcggtgatt ttttcattac tacctgcctt tattattccg
    13501 aataccgtca tcattataaa attctccgac ccattttcgg actattccaa cagctttttc
    13561 ctctaattct tcaactgtta aattattgtt tgacaacatt tcatcggaca ttgcaattgc
    13621 ttcgcttata cctacagcca attcacgttt atcgcgtatg ccaaaatcat tttcgttcaa
    13681 cggggcatcg cctctagctc tcttttttag atgatcaaac ctggtatttg cagatgcatg
    13741 tatagagagc aacttaacca ggccaatagt tcgcaatatc tcgacttcag gaacgctcct
    13801 gacgccatca atcacgatat tctgattggc atcacttcta acctctcttt tttcaatttc
    13861 atttagtatc aactgagcaa ttgctcctgg accaatctcc ctcctcaatt ttaacattaa
    13921 cttacctaag ttaatatcag tcggctctat gttctttcgt ctggcttcat ctcttactac
    13981 atcacccatt gctatcacag agaatccttt tttctttagt gagtcggcaa cggtagattt
    14041 accagaacca ggcattccag taagacatac gatgaatttt gtctttttaa tttttttatc
    14101 ctgaaaatcg gcgtttctgg ttccagtttc gttcttcttc ttcttttgcg tgctatcttt
    14161 ttctgccata tttgcgacac ccctgccgcc tactcaagga atctattaaa ttttcgccgt
    14221 gctattggcg gaatgaaatg tatatttagt tattctggct actgccagtt attccaaaat
    14281 aatataaaag tatatgaaag atactttggg taacttgaac aacatacggg cctttattgc
    14341 agttgatgta tcaaacaccc tcgctatcga aaagctacaa cacgaattgg tagctgacgg
    14401 cgaatggaca ccagagcaaa tcagacccgt gaaaaaccag aatcttcact ttacccttat
    14461 cttcctagga aacatacgcc tagaaatcat cgacgaaatt aagatcaaaa tatcggaaat
    14521 acaattcgag ccaataaaaa taacgttcac tgggatcggt ggattcccgc accctaactt
    14581 tgctagggtt gtctgggtcg gagtagacga aaacgggaac caaaggcttg ttagtcttgc
    14641 agaacaggta gtcataaaat tgtcagagat aggcttgacg ccagacaaac cgtttacgtc
    14701 ccaccttacg gtatttcgcg taaaaggaag aagtttgaag atccgaacag agatattgga
    14761 taagtacggg aagaaagatt tcacttcaga tattattgac aaattccatc taaaacgaag
    14821 cgatcttact ccttcaggac cgatatatac aaatatcttt acggtgcatg ctagatgaaa
    14881 agtattagca atattatttc acaagcgcta aagcaatgcc agccctcagc tgaagatgtg
    14941 gaaaaactaa cgaagatcgc aattgaaaca aagagccttg tgagtcgtta catttctccc
    15001 aaagtagtgg atgtcgtctt tggaggatca tttgcgaaag gaacatggtt aaaggacgaa
    15061 gtagatattg acattttcat caaaattgat aactcagtca atgataaaga gtttggacac
    15121 cttggcgagc aagtagggtt gcaatcgctt aagggataca atccatatct tcgctattct
    15181 gatcatccat atgttgaagc agttatcaat ggaatccgag tcaacgtggt tccttgttac
    15241 gatgtactga agggtaaatg gaaaagcgct gctgacagat cgcctttcca tactgagtat
    15301 ataaaaaaca atttagacga cgaccaaagg aaccaggtaa ggctgctgaa gaagtttttg
    15361 aaatccgttg gtatctacgg agcagatatt gccacaggag ggtttagtgg ctatgtcgct
    15421 gaaattatga ttttgaaata tggtatgttt gagtccgtat tgcatgcaat gtcaaatata
    15481 ggggtagaaa acaatgtgat ttcgatagat aagcctgatg aatacagtat aaagaatttt
    15541 gaaagtcagc ttatcattat cgatcccatt gatcatagaa gaaatttagg gacggctatt
    15601 tcggctgaga gtgttggcaa gttggtactc gctgccagat cattcttggc aaagccatct
    15661 tttgattttt tcatcaaaaa tgaaaaaaag ctctctggaa attatgaggg actttatcct
    15721 aatttagtta ttatcgaatt cagttataaa aggcgtagtc ctgatgtcat ctggggacag
    15781 cttaaaagaa gccttaatgc aatttccaag caactagaac ttgcgaattt taaagtaatt
    15841 cgaagaattt gcgtaacgga tcaacaagaa actgccgtct ttgtgttctt attagattcc
    15901 gttacattgt ctgcctacac agaaaaaatc ggcccgaaga tatttatgag aaaagaaact
    15961 gccaatttta tcttaaaaaa tcaggagaaa tcgctaatca cctgggttga caacgaaatg
    16021 agggtttcaa ctttaattca aagagagact acaaatgcaa agcatttctt gaaattgctg
    16081 ttgaccaaga aaatcgaaag tacaggtatc actaagggac taaagggaga catccaaagg
    16141 ttatttcgta tatattcggg cgatgatcaa aaaataaatg gactcgcaaa ggaggcagtt
    16201 agggatctta tcacatcgga tcaacgcatt ttgtaataaa gtcgatctaa attgtcctga
    16261 gcttgttcct gtcatttcgt atccaagaca aatcaacgac agagaatata gcggcnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnagaata tagcggccgt ttgaaagaat tagaaattat gggtgttaac tcaattttac
    16561 ccgttggaag cacgaaaatt ggcaaattta gtattctagg aaagggcggc gtgggtctcg
    16621 tggtgaaggt cgaatccaac gatagaaaaa cctacgctct caaaatccga agaatcgatg
    16681 caaatcgcag gtcaatggaa agagaggcca ggctgcataa aattgcaaac ttagccggtg
    16741 ttgggcctta catttacgca aactccgaaa attttatttt aatggagttt atagatggtt
    16801 gcaacataat tggttggctg aaccaccaaa atattaacgc tgatcaagtt cacaaggttg
    16861 taataagtac gctcgagcaa tgttatagct tagatagggc gaatcttgac catggagaac
    16921 taagctgctt agaccatcat gtgctggttt cagagagtgt taacgctcac atcatagact
    16981 ttgagacttc cagcattgat agaaagacac gcaatgtaac ggcggctgcc cattcgttgt
    17041 tactaagcgg aacagtttca agacgggtca ataacaacat acattttcct gagcgagaaa
    17101 aaattattca attactgagg atgtataaat ggaatcaaac tagattcagt ttcgataaaa
    17161 tattacaagc gttcggttag gccgtctgct aaacttgtct ctgctgatct attggtctca
    17221 tgatctttta gaggagtaga catacataac aacgtgatgt ttgtggtgcc agcagactcc
    17281 cctatcgaat tcgggagaaa aaaggttaag cttaaaggag gtacatttat tgacggtttt
    17341 cctagtgttg ggttggccaa cgccattgca tcggaatggt ttgttcattc tctagcgact
    17401 gagcttgtcg cagttcttga ttcacctggt tttccgtcgt tatcaataat tcaaaaatgg
    17461 tactccaaat tttcctgcaa taatctatgc caatgaagac ttgaaacttg ctatttttgt
    17521 ctatgtatcg cctaatatcg aaaacaatga caattttgcg atgggcaatt gaggagtgtg
    17581 agcttgtcat aagcgctgct ggtttgcagg acgactacac tcaagaagaa tatgatatta
    17641 aacgattgaa cgagtagttt atgctgctgc gagcaccaat accgctctaa agtgccggaa
    17701 tccaacaact gcctaacagt tctgtaacag agattcctac actattattg aatgagggaa
    17761 catggttgaa taaagatgtg attgttttgt tagtcaaatt gttgaaagat gcgccagatt
    17821 ttcgggcggc agcagtagtt tcgcaggctg tcagtaaaca gtgccgggcg tctcatgtga
    17881 tatcatgtca ctactcaagg aagcagaact aatggaaaaa aacctatgaa ggatcaaaag
    17941 agaacagaca aaccctaacc caggactgta tggataataa gaattgcagg atgcataaac
    18001 aaagtaagca agtcagaaat atgtgaataa tatattctgg catgggaggc agaaatattt
    18061 agggatgaaa tttcggatgc tagctaaacc ctgcatattt tatatcactt gacaatatga
    18121 catacggaat gcaagatttc aagttgctct aaaggatgtt gtagaggatt aggcacacgc
    18181 tgtaagaaat aacctgaaat tgcactcaaa cgtggcatta attctgcgag tagggactta
    18241 aagtattcgg cgtagctgtg gagtgcggac tgacgtttta tgatcaggac ttagtaacga
    18301 ctcgtatctt tacttattaa tgacaattcg cacgaaatcc agatattggg ctcggtaatt
    18361 tagaagtaga tatacaataa tggccgaaga tacatcatcg accgcttcga atactatccc
    18421 gtttcctacc ttgactattc tatatttgcc tgctgaggca gagaacatta tagaagaagt
    18481 gagtcagaag tattgcaaca taacagtaga ggactgcaat gggttcttcc acggcggcgt
    18541 aaagagacaa tacaagaaga taactatttg gagccaagga attgatagcc tttggtttaa
    18601 tgcaattatt gctagaatta aggaactggc cagtgtggag tttgttccga ttgtatctgg
    18661 agggataatg tactctgtct aaactacagc ttggagttat tatgccggtg atggccaaac
    18721 tgtcgtgtcc aatctgtagc taaagttatt attttcctag aaataactcg gttgtttaat
    18781 gttcacttta gcgagttatt tttgaaaaga caagtttaaa atagtaatca tttcatgtct
    18841 ttcgatctta tttgcatctg tattgatgca agaatgaaat atattattag acagctgcag
    18901 atgtttctat catatttagt aacgtatcat ttcggtgttg ttgcagaaac atatcattta
    18961 aagaaacaac gagataagca agaggactaa ctattttacg attggtggtc ttattagaac
    19021 tctttgatga tagagtccat agagcgattg ctgggaaggt tgtattgtgg gttttggtat
    19081 attactcatt cataccggat atccctgcaa tccagcaaaa cgctatgaaa gtctgttgtt
    19141 ttctcctgca ggacttcgtc acttccgaat tagtttaaga ctacctatac gagccctagg
    19201 cgggatttgg acccacgacc taaggtttac gaaaccttcg ctctgccagg ctgagctact
    19261 agggcaatca aatagaggca gttgatcatt cattatttaa tccttcatgt ctttgtataa
    19321 gaataaatgt gacaattttt tgaataaaaa ttaagacaaa atcaagttaa attaaatatc
    19381 accggtcttt agtcgtattt aatatgaagg tctcaatctc tggcattagg ggaatttatg
    19441 gtaatgacct aaatttgcat gaagttatca aattcagcag attattcgca tcttgcttaa
    19501 ttagaccaaa agggaaatgt gtgctagcaa gagatacaag gcattccgga aaaataattc
    19561 tccaagctgt gtcggcgagt ttaatggaac aggggattga tgtatacgtt ctcgatgtag
    19621 cccccactcc tatggtattt agggagacaa ggaaatatgg agccggtgtc gtcgtaacag
    19681 cttcacataa cccgctggag tggaatggac taaaattcgt agttgatgga agagggattt
    19741 acgagaatga attggatctt atgcttaaag gcactacatc tacatcagac aaatatggca
    19801 aatcatatga tatagtgtca gaatatttga acgaaatttt tgatttggta gtccaaacgg
    19861 gaacaagcag agcggttaca ttaggatttg acctcggcgg aggcgccaca tgcaattatg
    19921 tcgagggact ctttaaaaaa cttggccaca aattccattg tgttaacgat gtccctggaa
    19981 tatcctcaag aggtcctgat cctactatcg atgctctaaa tgaattacgt actttagtac
    20041 aggaaaatga gctagacttc ggatttgcct ttgatttaga tggcgataga ctcgtagtag
    20101 ttaataacag aggcgagaaa ttgaatcccg attcaacgct gttgctatgc atagccagtg
    20161 cactcaagat gggaatgaaa aaatttgtaa ccagtataga tactagtgtt tctgtagaga
    20221 aattaataaa gtcgcatcat ggtagagctg aatattctaa agtcggtgag gcaaatgttg
    20281 ttaagagaat gttagaactt gacgcagatg ccggaggcga gggaagcagc gcaggattta
    20341 ttctgcccag attcaatatg tgcagggatg gatttcttgc agctgcgact gtttcttcgt
    20401 tggatcgggc aacgataaac gaatgtctga gattggcttc agaatatgtg caaatcagat
    20461 caaaaatttg tgtcaaatct agtgtacatc tgaaattgct cgataaacta cttgatcaat
    20521 tcggatcaga atcatctcaa attttgacga ttgatggtgt taaagcaata tttgatgaaa
    20581 attcatgggt tcttgtaaga ccatcaaaca ctgaagatgc gattagaata tcagttgaat
    20641 caaaagctaa caacgcacaa tccctttaca agaagataat ggagaaggtc aacttaattt
    20701 atgagcgtgt taaatgaacg agcgattatc gatattatta tttctaatct cgggctaagg
    20761 ggtaataata aaagaagaga tgatgacgtg tcaaccgttt cgctgcgaaa catgaaacgc
    20821 atgaagagtc tcatcataaa atgtgatatg ctaattgaga gtactgacgt gccccctgga
    20881 atgtacccat ggcaaattgc caggaagagc atatctgcct gtgtcagtga tctatcagca
    20941 aaaggaatta aaccgcaagt atcggtaatt tctgtaggga ttccaaagaa atattctaag
    21001 actgagattg aggatctggc aaaggggttt caaattgctt ccaaggaatt tggtgtcaag
    21061 atagtgggtg gtgatactaa cgaatctaag gagctaatta tcgattgcag tatgatagga
    21121 ttttcagagg attgtgcatc aggacttccc ggcagaaacg gtgcaaaacc tggtgacttg
    21181 gtagtcgttt cgggagaatt tgggtatacg gcgtctggac tgaaaattct tacgaaaaac
    21241 gcgaaagcgt acggtgaatt caaaaataat gctatctcat ccgtgatgaa gcccaaacct
    21301 gcgcaaaagt ttgggatatc tcttgcaaaa tatttctcag catcaatgga ctcaagtgac
    21361 ggtttgagca tctctcttta tgaacttgca agacaaagca atgttaactt tctaattgat
    21421 aacattcctt cagcaaaagg tgtatgcaat tttgctagaa ataatcagct agactacaag
    21481 cgattgattt tttgcggtgg agaggaatat gagatcgtcg gaacaagtgc gccggcaaat
    21541 ttgaagaaag caaaggctac agccagacgt ttaaaattga aattgtttat aattggcaag
    21601 gtagaaaaag gagaggggaa agtatttctt ttggatagga agacaggaaa acgcgttcag
    21661 ctcgaagata tgggttacgt tcacctaaac cataggagaa aatccggtta gaagagtccc
    21721 tttgctgctg ttgtcgttgg aattgaagtt cctcctgata ccctttgcta catttctcca
    21781 gataattata ccatgatctt tttaaatacg tatagaatcc aagataacgc cgatatcgtc
    21841 tatgatctag gacacgttcg gtaaatagcc gtcgcagact gggtgtaagt cccagtatcg
    21901 gcccattttt ctaaagcaat ttgttagcct ctcggtaaca agaataaatc caacatttcc
    21961 tttagatgaa atcttggcca cctaataaca ggttcaatat catctccaaa agagaaaaag
    22021 attctactcc tcgcatcgag gccagctcta aacccgaggg taacacatta accaaacttc
    22081 tttaagcctg ttcagtttta gcggtaccag cgccatattt ggcaaaggtc tgaagaatct
    22141 tatcaagttt agaaacatca atgtcaccat gtgttaatcg tgctattctt atacactctc
    22201 taatgtttgt actcttgagt ctatcgaata ctgcctcggc aataatagta gcaacatctc
    22261 tatctagtcc ttcctcccta tccagaattt ccacagcaat atttacaaat tcttcctctg
    22321 tatatggctt gaaatgcata tctctaaacc tagttagcag tggcggtaat agcttctccg
    22381 tactattaca tgaggcataa acccatgtct ttagttgagt tgttcttttt tgattgtgtt
    22441 ttaattcgga taagataccg gactccatca gattcaaaag acttgtctga tctcgcttat
    22501 tcatcttctc tatctcgtct attatgaaat acctcggtcg atattcaaac agataatcga
    22561 atatgccaga ttttgtagaa ctactcccaa caacgtagta tgacctttct agtttagtaa
    22621 gtgaactcat aaacaatgac ttggctgaag caggtggtcc aaccaggagt agatgtactg
    22681 gcttgtctgc ccttatagac atatcaaaca gttcttttac atcgtcgaaa ccaacaatgc
    22741 catcaaatag actacgtttt tgccctatag ccggtttaga atatcttcta ttaccatcaa
    22801 gttctgaaac cgggatgtta ttactattct ttctccaacg accaaatact ctcattccct
    22861 ttaccttcta tcccaaatat agatatgaat caatcctttt tcaaactgat ttagcattat
    22921 cctgacactt tgttccttca tctttttctt gtagaaaaat agtaccaatc atccaaccca
    22981 gcccaatcta acctcattgc cataattcca gttacataat ccaacaatta agaccaacct
    23041 tgtcaatact agaaaaatat aatatattga agtatattat tttccaaagg tgtgactggg
    23101 agctaggaag taaaatgtct tgcgccttta agaaagtgag ttagggcatg atattcatct
    23161 ttttaaaggg ccgctaccgt caacctattc tattatatgg tcatttcatg gtgttgggaa
    23221 tctccgtgcg actctatttc gattgaattt gtgataatat aaaaacagtt aaaggcgacg
    23281 gcattaagat tattcacatt tctaactacg ttaatatagc cgccaagtaa taaaattacc
    23341 tctctattca tgtccacaga tatcgctgaa gttacgcaat ttaagtgggg ggtagcccat
    23401 atattcagta gttataacaa tacattagtc catataacag acgttagcgg cgctgaaacg
    23461 gtttctttca gttctggagg gaggcacgta acagccgacc gatatgaatc ttcaccatac
    23521 gctgctatga aatctgcagt tgccgctgct gatgcagcca aaacaaaagg gatcaacgcc
    23581 ctccatatac gtgtaagagc agttgggggt gtggggccga ggattccagg ccccggagct
    23641 caagcagcga ttagagcttt ggcgcgggcg ggtttccgaa ttggtcgaat tgatgacgtt
    23701 actccagttc ctcatgacac aacacggaag cccggtggaa ggagaggaag aagagtttga
    23761 gatctctcgc tggtaccacc ctaatctaaa tacaagaaga cattcgtacc tccgcgaaaa
    23821 attaagataa ttgtttgtta atcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 nnnnnnnnnn nagcattatc agttagatca aactaactac gggaccgtga ttatactaaa
    24121 attttcagcg aaattgcgaa gaactatgtc tacggaggaa gagctatcgg ttggctcgtg
    24181 acttaaagat taaaagctga tgttgcagct aagcaaaggg atatggattg aaaattatcg
    24241 tatagtacat aataagtacg gtatattacc aattattgat aatcctccct ctccctatcc
    24301 taaagtagct cccttggcta taaggaaggt aaaacggtta cagaatatct aaaggaagaa
    24361 actatgtctc aaaatgctac tcccttgcga ctggaagacg tagctgaact cgagcgatat
    24421 ttgataagta aacacaaaag atccagaata gaatctgtgt ttgacatatc cttgtcaatt
    24481 ctatacgtgg ttgcaggtaa caagaacgaa cctatgactg acctacgggc gtccctcgaa
    24541 cttgtgactg atgacagcca ggattcacta tacgttcagg attacttcaa aaggcttctg
    24601 tgaaggtgag gaggttttaa aacgactgga aagttattaa cagtagtttt ttgggacata
    24661 aagggattct ccaaattgtg tgacattctt aaaacttatt cgacattact ggttccattt
    24721 ttgagggaat tttttgacat ggcaagaaaa ataatttttg aatatggtgg agtattggac
    24781 aagtatatgg gagatggagt aatgggtctt tttgggtttg aaagtaaagc cggagaatgt
    24841 acagggaatg ctatttgtgc tgttgcggca gctcttgaat taaaagaacg ttttaaagat
    24901 ctccaagcaa aatggatatg tatatgggaa aaacatgttc cacatacaat aacaatcgga
    24961 ttgaaatgtg gaattaatac tggttatgcc atagttggta acataggaac tgagcggaga
    25021 actcaattta ctgcactagg tactactgta aatatagcaa gcaggtta
//
LOCUS       DAOR01000151            3140 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_109423, whole genome shotgun sequence.
ACCESSION   DAOR01000151 DAOR01000000
VERSION     DAOR01000151.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_109423"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          952 a          631 c          555 g         1002 t
ORIGIN      
        1 atataatacc actgagaaac tggcctgtca ggaaaaactc tcgatacggg acatataaaa
       61 aaacattctc cgccgctata cgagatagta atgaataatg aaaacaccac ctaacaataa
      121 ttcattgcct tttcaattgg atagagtcgc gttctatgga cctacattga cagaaaattt
      181 aaagatgttc gatattgatg acatctcttt gaaggaatac aagatattgg actgtccttc
      241 gggttcttcc tcatttgttg cagaagcaaa aaacaggtat ggaatccatg caattagatg
      301 tgacccattg ttcggtaaag atttggaaac ctcggttgaa cgaggaagac agatattgaa
      361 tatataatgc aaagagtttc acgtacacgc aatctttaca agtgggattg ttattcgtct
      421 gcaaacgtca tggaaagaat acagaacaca agcaattatc tgcgagcaaa catgaacagg
      481 gcaaaaaatc aggtaacata acaaatattg acatgtcatt ctatatcaaa cctttgaagt
      541 tgtaaaaatg actttcgcaa tactggaaat gatggaactg tgtttacaaa gattcgagtg
      601 aactaatcat tgacacgtta aactgataga tcttctagag ctgtttgatg atcgatagta
      661 atgttaagtc tcctgctatt ggctctcaat ctccaattga agaattgaac ataaagatgg
      721 cgcaactttc actacgcgtg tcttgcgtct aataacttga acagtaatta cacttatttt
      781 tgtttatgat ctcccatcta tgactaaagg acataaagcg agttgtcctt aaatgaggtg
      841 aagctgtttt gggttgaata gtcttgatgg atcagttcat gatcttattg ccaaactcca
      901 aaagagggtt tatgaaaggc tttaagaacg gattggtcat tcttgttgct aaattggaat
      961 tacaactaca acatatgaca ccatacatat ttgtctgctt cagaattgag taggaatact
     1021 gtacaacgta gtcatatgct gatcctcatt agataaattt gaaacacata atcaaaaata
     1081 tgcgatcaca gcaggtttga atcttatagt gaagaatggt atggcctttg taacaatcgg
     1141 atcattagtg gtatttgaga ttatcgaaaa ccggatattt ctaaataaca gctgctttga
     1201 tctcggccat ttctagcgcc ctcgtaattg ttttagaaag aagaatgtct catccaagat
     1261 tcaagaaatc agaaatttga ctacaggttc ggaagcaaca attggtgagc caacaatggg
     1321 gcgtaattag ctcagttctt tcttgattct tactacaagt tctttcatct gttccttagt
     1381 ctctttatat ccatcttgaa ttagctgtct gattgtagtt tttgaaaagt caaatgtctt
     1441 gtttgaaatt gtatgaccgt cgttctttcg ctccaaacga gcaacaaaat caacatctac
     1501 tctccacttc agtaaatcac catatcttac ctgtttccct gtgacaagat aaacaccctt
     1561 agttttctca gtcaaaatct tttgcagtgc ctctttgctt acacccttct cttcggctaa
     1621 tctaattaga gactttgcga atgtcgcgaa atcagacata ataactgcaa cattttcatc
     1681 aaattgtgtt ctgtcatgat atatgatgtc attctttctg tcaacaacat cgtcatagtc
     1741 agaaggaaga tattcttgtt tagctggatg aagatttatt ataccgtatc tgagtctggg
     1801 tatattattg tcttctaatt tcctcactct atgccaatat tctctgtgag ccatgacaac
     1861 ctgccttagt ggtgtattag ctaataggcc accatcccag aaaaagcgca agctgttact
     1921 gccgttcgaa gaagaaggcc gattactatc ccctatatct gcgttgtcgc cttgccctgc
     1981 cactgccagg gtacgggttt ccacatttag tctagtatag tcataattta ccggaactga
     2041 acagcttgct aaaacgaaat ctgccttaat gccgtcgtcg tatcttataa cgtgttcaaa
     2101 tttctcatta ttctccggat tcttcaccga accaccaaat ttcactctac cgtatcttcc
     2161 gtattctgac ttccttgttc catcttcctt ttcgtagcta tcgaaaacca ctggagtagc
     2221 ttcctgtaca tccacagcaa ccagcaagag tcttggttca tcattttcga agcttgtgga
     2281 gatcggaaac tttgcaaact tttccaagct ttcttttaat gatctgttgt catagctata
     2341 ccatgtattt gatggatcaa aaaatctatt gtctgacaat ggtatcttcg gtataaacac
     2401 gttaggaact cccttcaaaa taaattcctt agtgctaaaa tatcttctag cagactctct
     2461 tgaagcaatt cgtttatcta atctatgcca agaatcccaa tagtgcgtaa agtaaggtat
     2521 gttttcaaca tatgattgtg tagaaagata ttcccaaaac tcgacaagtc tttcgccaga
     2581 tccttcctat gttttatttt cctttacgta gcttaccaat atagcagcgt ttatcgcgcc
     2641 tatggacgtt ccagctacga tatgaaatat tggctcgttt cccctcccct ctgttctaaa
     2701 gtattctgaa agatcctctt tgatagcttt gtaagctccc gcttcatatg caccaagcga
     2761 gcctcctcct tgaaatatta aagctctatg tatgttgaaa ttcatatttt taggatcagc
     2821 tgatatacac tataccgcta cacctgcggt atatttatta ttattatctt tgtcggctat
     2881 tctcgtaaag aattacccaa cttcaataaa cttgcaagtc atctcccttt tcaccttcca
     2941 aatacaatct aaacagatag atgattatac aatctgagta aaggagatac gacaggaaca
     3001 ggtcagaact aaaaatggta aataaagcat tactgaatga tggtagtctg ctgtatcggc
     3061 tcggtcaacg gtatgttgaa aaggggttcc actgttaaag gatcacttgt caaggagcta
     3121 gaccttttaa tgtgaataag
//
LOCUS       DAOR01000152           46155 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_15727, whole genome shotgun sequence.
ACCESSION   DAOR01000152 DAOR01000000
VERSION     DAOR01000152.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 46155)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 46155)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..46155
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_15727"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    24396..24589
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26359..26531
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        14143 a         8848 c         9050 g        13747 t
ORIGIN      
        1 aaattatatt ttgaacatca acacaatacc acagaaggct aataatagag ttaaggggat
       61 atgagaatag aaaaatttgt gttgaataat aagtattata cacgcaataa caacaatttt
      121 ttagataata ggaataataa tgaccttatt acttttgatg aattaatatc tgatttcata
      181 cgcgttagaa agaaaacccg agaactcttt aagccactta aactagagga tgcggtagta
      241 cagtctgata tctttggcag tccacctaac tggcatctag cacatgttag ctggttcttt
      301 cagaaggtgt tagaaaaaca cggtcaaaga ttggcaggag gatcggatag tgatagtggt
      361 ggcagcagcg aggtcaacaa tactagtaca actcgtcgcc gctatcgcca ttgtattaat
      421 ttagaatatc ttaattcata ctatcagcga tatggaaata tccttccaaa atctgaacgt
      481 ggcaaattcc ctaggccaac tgtacaacag actctggaat atagattgtt tatagagaga
      541 aatgttattt catttttgaa agagaagggg aaagaagaag atgatctcaa taacaaagga
      601 acagcatcag catcaaggtt agaggaattg aagtacgata ttattttagg aaaccagcat
      661 gagatgcagc atcaagagct tatgatctat gattttcaac attatttcca gcgcttccaa
      721 gaccctgcag acaattacat acctgttaag gcagtagcac aacagacagg gcaggcagga
      781 ggagaacagc gtggaataag acgagcggtg acgaggaaag aaggaccaga attaagaaaa
      841 atggttgaga tcccaggagg aatttacgaa ctgggtttta atggtaaagg gtttttttgt
      901 tacgataacg agcttccaga acacaaagtg tatcttcagc cttataaaat agatgtaaca
      961 ccagttacca atgctgacta tattgaattt attgaagatg gaggataccg cgactttgga
     1021 tattggttag ctgatggatg ggatttagtt aaagagcatg tttgggaagc tccactctac
     1081 tgggaatctg ttaaaaaagg agaatacagt gatattatca gtaataaata tgatgatggt
     1141 agtagtacta atagtaccaa atgggtgaag aaggactttc gcggtattca tgaaattaat
     1201 agagacgaac cggtcgtaaa tgttagctat tatgaggcgg atgcgtacgc cagatgggct
     1261 ggaaagagac ttccaaccga agctgaatgg gaaaaggcag caagctggaa tgatggtctg
     1321 cgtagaaaaa ctctttatcc ttggggagac gaaagacccc agcctaaata tgccaatttg
     1381 caagaatcgt ctctttgggg cccatctagt gtgggttcat atccggaggg gaagagctat
     1441 tatggctgct accagatgat aggtgacgtc tgggaatgga catcgtctga gtatgtacta
     1501 tatcctggct tcaagccaaa attctcagaa tataccgata aatgggccat aaatcaaaaa
     1561 gttcttagag gaggatgctt tgctaccccc gccactcaga ttaggaatac ttatagaaac
     1621 tatttcaaac cacacgaaag aatactattc tcgggattca gatgtgccaa ggatatttaa
     1681 ctcagaaaaa aaatcaagga aattatagaa ttcattagag taaacgacct aagattccga
     1741 taagctccga tatatataca cgagtaatgt tttagactat tcggcgatac atatgataca
     1801 ttgactcctg acgtcaacaa aggggtacta acccttcaca tttcctcaga tatacgccta
     1861 tacgcctcac cctgagacag attatcaggg caacaacccc ttggaacgaa ggtggtatag
     1921 cttgtcgtct ataggatgcg atatatgtct atttatcaaa gaaactagta gtagattctg
     1981 caagaagaat aattatcata ttgtcagagg gtcgtaatta acattgctct acatagtacc
     2041 cgatttcgtt ttaaagatag aactaattca gtgttagtca aatcgtaatc agcagtctgc
     2101 aacttagctt attttgctgc ccaaaaaaac gtcattttgg tgtgtctttt gaccggaact
     2161 gcaattctaa tggaggcatt gactacgtag taacataata caggcattat ctcactcacc
     2221 ctaccacaat tggatttgtc agcaggtgat agccagttgt tgagaatgct atcaagtttc
     2281 taccggacat accggtaaga accaaagcta ttgcatacta aaccaaccag cactaaagga
     2341 taacattcct gtttacataa tatcaggaag cgatactcta cggcgaatgt gtattcatca
     2401 atagaataat acttaagaat aacaggttgt tttgtctatt ataattagca tgtatagcaa
     2461 gaaatcctac tccttctaat atagaataat aatttgggta tagaattggc ctatgcgaca
     2521 agagaggttg ggagttttgc tagagtcgtg tgttctatag aatctcgacc ctatcactct
     2581 ctaagccgtc tacactcttt cagtactaaa caataactac ctgtagaata ttaatcacgt
     2641 ccgtttttgt gtctaaaggt aaaagttacg ctattcacaa aaacagttaa gctctcaaaa
     2701 ttagaacatc acgtcatgat gtcatagcaa ggagtggatt gaattccact tctccttttg
     2761 gtactgtaca caccagagcc gtttggttac tatctaacct ttatttctga ctgatgtaat
     2821 taataggtgt tatttcatcc attgtgttct gtatttgcaa taaacgcata cctttctctg
     2881 ttgttctgta agtccgcatc ccctcttcat attctatcaa accattttca actaaaagcg
     2941 ctagatattc tcttagttga ttaaaagata ggtatgcttt gtacattagt tttgttttag
     3001 tggctcctcc accatttgcg gcatccaaaa gtagcccgac tatgtcactc ctgcttctgt
     3061 atttcatttt gttctgagat agggaatgaa aaccaactat ttatgagaag ccctacttag
     3121 tactaaagtc tatgtcaagt tctattatag gatactattt cattgtttta tcgagtctaa
     3181 ccatctgcac gtacaagtaa gcattatact atagaaatta tacattttct cttcaactgg
     3241 ttgtaagatc ttgtgtagaa tgggggaatt gtaagataga tcaggttaag gcgatattta
     3301 ttcttcttgg atgacgatac atttacgtcc aacggacttg aaaggtatca gatcccaagg
     3361 agaagaaact acgattttca cttttgtgtt atgcataatt tgattattat aacgtgttcc
     3421 cattgactca cacatttaag aggtgcatga aagagtatga agatgctggt ataaatgtga
     3481 gcgataaatt accattataa gaagattgag atattcgaga cagacagatg ggaataaaga
     3541 ctgtcgttaa gaagattttg atatcagatt taccgcacct atcattggcc acacgaaaaa
     3601 ggacgacaag ccgaaacttc ccgtacttct acttcccgta taggcggata tttatcataa
     3661 ctgtaaaacg aacaagatgg acaccatttt ttataatctt tattgtcatt ttttatttat
     3721 tctcattaag attacacggt accatggtac actagttatt acagcagtca tctttatttt
     3781 cacatttaca attttctggt tcctgtaaac agtatggaca tataatagag ccacacccga
     3841 cacaagtgtt gtcggatcta ttacatcccc aatgcctaca ttcatagtta gtcaactcta
     3901 ccgttccccc atttactctg acaattacac ctacacgaca gtgcacttat ttaatcatag
     3961 tctctgcatc ccttgaaatc aagtttcaga atttgttaac aatctcatcc gcgaagagat
     4021 cctatgcctc gtgttccctc gcacttcaaa attgataatc agatactcta ctctctttgt
     4081 gtctattcag aggtaccttt aatgacttgg cgacttttcc ctatacgtac taataatgtc
     4141 gttagccata acgaaaacac cttgagttaa agacaacagt tgatgcaact taaggtagca
     4201 taattcttga ccatgagata acattatcag tgaagaaaaa agtcattgtt gttacgatta
     4261 gtattccaaa aaaaagtgtt attaagccat tcgcagattc ttggtagaca tactaaggta
     4321 aaaaaccaat ttaatgccat gaatataaag acaagatttg ttaatgtatc aatagtctct
     4381 gaagctagag acattagtgt attcaaagca accaagaatg ttcttgatag acttgaactg
     4441 ttccggtttc ctggagagtc tacggcactc tcgaataagc gaaccatatt cgaatatcaa
     4501 gcctgaacat atcgcagagt atggtattgg tgagctgtat gataacaata atagaattga
     4561 cagatcgaac ggcacaatgg aggggactaa tactggtaac ggcattggaa aaacataaga
     4621 tactattaac agaagtaacc cggattcatt ataattttgt caaacaacat caggcgttag
     4681 agaatcagac acccgcacag agggcaggaa tagggattaa gggaaatgac aaatggatga
     4741 ccatgatgaa gaatgcgaag aattcaagat gaaacctaaa ggatctgcag aaaagttgga
     4801 caaaatgttt aattactgat tgtttgcaga aataattagc cattgtttga gaaaatgcag
     4861 gcgttaatcg atattgtcaa gaaaaaagga attgtcgtcg atgacataac attatccagt
     4921 ggtgaaaaaa gtgaatatta ttatgattta aagaacgtct cattagatcc cgaggcaata
     4981 gacatcatag gggatttgtt attggaagaa atagtgctta aatatggtaa ggtcaagtct
     5041 gttggtgggt tagcgagtgg cgcaattcca ctagtaacag cagtggttat gaagagctct
     5101 aataacgttg aaggcataaa tgggtttttt gtaaggaaag aacgtaaccc gcatgggtta
     5161 cagaaaataa ttgagggtaa gataataccc ccagttgtaa tagtcgatga cgtaatgact
     5221 acagggggta gcataagact agcaatagat gctgtcagag aagggggatt taatgtcgag
     5281 ggcgtcttca ctgtcttaga cagagaagaa aataatgaat tgagaaagac catcaaatgt
     5341 ttctctttgc taaagcactc tcaatttaag cccttcattg aagaacacgt aaagagaaag
     5401 aaaaaaacaa aaatagaaaa ctagcctttt accaactgtt ttccaacgac tgatttaaac
     5461 aacgtaagac aacagaaccc attttctgct ttcagaacat tgtttggagt aaaaccgata
     5521 aatgtgtcca taatcaagac catgttcaac aaaaaaagaa caaaaattcg taacataacg
     5581 tcgtaatggg attctagatt aagggatcaa aaatattatc ttggactctg aattaattat
     5641 accgtaaaat gagatgaata aactgatatt gacgatgttt gtacctgtta tagtcctagc
     5701 atttgttcca aataacggta ttgtcatttc agcgctagcc ataagtagcg gcagttccgg
     5761 gcaagtatcc aagaggcagt aaacactagt aatcctgaac ttgacaagca agttaataaa
     5821 ttctatgatt gtgtttctaa aataactcac gaaccccaag aacctggtaa agcagaagtt
     5881 gataactgtt attttcaggt atttggagga agcatcgaca atagtagcgg cattacattg
     5941 ccttcaaatg gctcgccgtt gagcgtaagt aatagtcaag gaagtaatga aaattcaggt
     6001 cagggcagca atactggaaa tagcggacag attgtaagcg atggcgtaca tagtaagatc
     6061 gtaaagcaat tcaggtgatg gaaaagtatt ctatcaccta ttcaatgaca gcagaaacaa
     6121 aaaaggaaaa tggcaagaaa aaaattttgg aagtagacga tggaccagat gtttgtacac
     6181 tgctaaagaa ggttttaacg gagaatggac tcaaagcgga ttctcatgct gatcctattt
     6241 tagctttaga gaattttaaa gcaggtttat gcgacttact tttattggat atcaaaatgc
     6301 caaaaatgga tgtcttgcat ttcccaggaa atgaagaaga tagacaataa gattatggtg
     6361 tgtttttaac atctagtgag atgtattgtg agaagtttag gaaagaaaaa gagttctcat
     6421 cattagataa tgttttaatc attcgaaaac ccattcgaca tgaagacttg gtagggcaaa
     6481 taagtacaat aataagccaa actacataat aatagtaata ataataggca tatgaaaaac
     6541 aaagtacgaa ataacccctt ccttactgtt acactaaaga ctttttaggc tattcaagaa
     6601 tcaaatatta tgtgaattta gaaaaaagag tatttcagcc gggagacccc acagtttcaa
     6661 tgaggccggc gatggctcca ttgtgttttc agggactcaa caccttcctc atgagtggat
     6721 gtagtatttg gattgtttta gcaacattgg ctagtactac taacctatat aagataattt
     6781 aaataaccta aaagataatc gtggtagaac aaaacgatga tcaccacaac catgatcatc
     6841 aggacgagca tagggaatcc tcctccactt catctgttgt tgcaaagaag cgtcaggatg
     6901 cgcataagga gaaactcaaa gtagctataa cttcggttgt agcttcagca ggtcttgcca
     6961 ttttcaaaat tataatagga ttttctacca acagtcttgg gatattatcc gaagctttcc
     7021 attctggact tgatgtaatg gctgctttaa tgacattgta tgctatacgc atggtaatga
     7081 gaccaccaga tttgaaatat acatacggat atgccaaagt tgaaagcgta tcaagtctgt
     7141 ctgaaataat actactattt gcagttgctg gctggatatt ctatgaaggt gttgaaagaa
     7201 tattcttcaa aagcatccaa cccgagatta cactattctc atttataatc atgtttgtat
     7261 ctattgggat tgattttgga agatctagag tactatatag aactgcaaga aagtatggca
     7321 gtcaagcgct ggaagcggat gcactccatt tcaaaacgga catgataaca tcgtccatag
     7381 ttattgtggg gctgcttttc gtattcttat ttcatattcc aaaggctgat gcctatgcgg
     7441 cagttatagt agcaggaatg ataatttaca cgtctcttgg tctgggaaga aggacattag
     7501 atgtattgtt ggataaggca cctaaaggtg catatcaacg ggtactagaa gcggtatctg
     7561 gattagacgg tgttgataag gcgcatgata ttcgcattag gaagatggga tcggaaactt
     7621 ttgttgatat gcatatcgaa gttccacgaa caagtacaca tgataaagca cataaagtag
     7681 ctacatcggt tgaagaaaag gtaaggggag tccttcccgc ctctgatgtc ctagtacatg
     7741 tagatgcaac agaatccgca agcgaaacaa ttactgatag gatcagacta gttgccgcag
     7801 aaacagaagg cataaagaac gttcattcaa tatatctatc aaagataaca ccaacatcta
     7861 gatcactaga atcgactaaa gaagcagtaa ctaataccga tctgaataat aatttatcgg
     7921 atctccacgg gataaaacga ggatcgatac ttcacgtgta tcttgatgta cagatggatc
     7981 gagatcttga ccttaagacc gctcacggca ttatagattc ctttgaaata agattgaagg
     8041 aggtaattcc ggagttaaga gacatcacta cacacatgga gacagaaacc agtgaatacg
     8101 tatcaatggg gaccgagaag aaacccgcca tagggtactt agaaagaata agaaatgcgg
     8161 ctctatctat acctggcgta gttgattgca aagatattgg ggtagtatac gttagtgatg
     8221 aacaacacat caccctgtca ataacgatca aatctacacc agaaaagacg attaagacaa
     8281 tcgaagacgc gcatggatta gcaacgagcg ttcaaaacct agttatcagg caaactggtg
     8341 cttctagagt cattgtacat gcagaaccag actaacgtac tatatggtat gcttatttag
     8401 tgccattttg caataatttc cgtttcgatt gctattgcat tttttttaga ataatagttt
     8461 gaatgctcca aaatacaaga agggcaattt tgtgttatca taatgaaagc caaaatcctt
     8521 actagatagc atgcaaatag gtattgattt aaatatatcc caaaatattt cttctgatca
     8581 ctcaggcatg atgccgattt ctcacggacg ttgtcggaca cagaattcca tcctaatcgg
     8641 acactatatc agatatccgt ccgtatgcta tatttttgca gaagtcacat gaatccgaaa
     8701 attatgcttg cccggcatag cctgtgccag cggccaaaaa gaaccattcc tggaaggacc
     8761 ttgactcata aactgcgtgt gataatgtat tagtctggct ggctcagcac agtgagatat
     8821 aatgaaacct aactttgaat ttccaagaga atcacgcatt ctctatccat aattctttct
     8881 taccattctc agaaattgct agtaaattgg atttcccata gacgtgtttt ctcttgtttc
     8941 gaggcagcgg aaatttccaa ctctgcaaaa tatttgaaac cgcaatcatc caacacgtct
     9001 tcaaaatcat atcgtcctac aaacgttcgc ttttcttgtt ccaagcagct tacaagagca
     9061 gactttatcg cttccgagta tctccgcttt ttactgtaaa cataccatag gttatgtgcg
     9121 ccccgtaacc tcgacaagct gtgatagatt tcgtcagaca tctgtgtata atagcgattc
     9181 ataatctcgg tgttaacatc ctcccatgac ggtgttctcc atgaggcaac ccgttcaaac
     9241 ccgtgtccat caaactctgc tgctaagcta ataccttgat ccaacgtcgt tacaattacg
     9301 tctgttttag cagcaatggc atcccgaaaa tctatcccgg gctccatcca ccccacgaaa
     9361 ataatatcag attttttttc aattccccag cgtgcgtcag ctgttgtgat gtagtcaggt
     9421 ttcggtaaat tacagccata aaatatgcca gcactgtttt caatacctgt tgcaccagag
     9481 ataaattctc gtatctttat tagaaaatct ccgtacccgc aaccgatgtc acaatattca
     9541 ggactattcg agacagcttt cgcgattgcg actacacgta atacatctat ctttctataa
     9601 gtaaaatatg tccctcgcaa caaggggaaa ctaaaacgtt catgataagg tgagacagag
     9661 tatcttctaa actggcgtaa tgattccgca gccatttgca gatctcttgt tctaaatggc
     9721 atcagcagag aattaggact taatcatgtt attttttcct tgctttgtat tgggttgaag
     9781 gacttatgca tctgggggat cgggcacatg tgtggaaggt gaggttattg tgatagcaaa
     9841 taatcatgat aataaatagg ccagaggctt ttgtaaagat agtgatatga tatatcctat
     9901 tctaattact ctaccttgtg aaaagtttca caacttttat gattacgaga ttaattagta
     9961 caaaagatat tgcagatgct gttaatgagc taagcataga tgcctgatat ggttgtgctc
    10021 ctagatgaag aaactgataa tgaaatgaaa tttttgtcaa agaatatgct acttcggaaa
    10081 tcgaaaatgc ggtaaacagt ttttttatat ctttttttat tcttctggaa tttttctttc
    10141 ctgtgaaagg atctacgtac ctatatttat tatcaatata aaagaaaatg acaaataatg
    10201 gaatgtaaat actatactcc gttgatagag ctactataga atttgcaata tcacttttgt
    10261 tataatgctc aaagtattgc tgagaaacaa atgcagcaga aaagaaagag catattccag
    10321 ctataattat attcttgtta aataggatga gatctttgta tttgccataa aaatttcctc
    10381 tctgttttat tatgccatct cagtccttcc tcttccttgt gttgtaaatt cgacctatcg
    10441 tataactaga tagccctgtt ataggcttac gggtagaaat ttatagtata tgagttactg
    10501 gaaaagtaat tatagatagc tttggggtaa atcatacgag caatatcttt agaataaggt
    10561 ttatctgtac gcaagcattg gtatcaatct taagtactaa ctgaaccata atatcagaat
    10621 atgttaatca aatcttactt cccataattg gttgcctttt gataaaagtg cggcaagcgt
    10681 ctccatataa tggaagccac actcatcaag taccgattca aaatcaaatc tatgtttggc
    10741 atataggcct tcatgctcct ctttttttag ccaacacttt aaagctacac gaaccttatg
    10801 cgatatttca ggccgtgtgt atacatacca gaggttatgg gctgtacgca acttcgtaag
    10861 ctcttctttc ttgctctttt tcgttcttag tgaagaagtg tagaatctat tcatcaattc
    10921 ataattgaca tctatccaag aaggcgttcg ccaccaagct attttttgaa atccgaattc
    10981 gtcgtattca catcctccgt ttatcccgca ctgcccccca gcgtcaaagg tcgtaataat
    11041 acatttagtg aattgcgata cggttttacg aaaatctacc ccaggttcca tccatcccac
    11101 aaatgataca tcaaacgatc ttttaccaaa ccattcagca gcggcagcct ccgaatagat
    11161 gtaatcaggt ttatgtttct gaaatatata aaaaatactt gtatcctctt caaggcctat
    11221 cgcatctgga ataaattggc gaattttgct tagaaagtcg ccatatccgc agccgatatc
    11281 aatataattt ggatctggcg atatcgaatt cgcaattgct ataacgcgca ttacgtcaac
    11341 tttccgataa gtgaaatatg agccagcgaa aaaggggaat atgtagcgtt cgtggtgtgc
    11401 tggagggtta tggtcattga attgcttata cgcaagatac gccgtttgca aatcatctgt
    11461 tctaaatggc atttcagtcc ctgtttttga aaatttggtg attccgccat attatatttt
    11521 cagtatttca gctcttatct tggaactaac ccaaaacgct tattaacgcc gcttataatt
    11581 ttggttttat ggttagaaca tatgaagaat ggtggaagac tgttcctgaa gatctgagaa
    11641 agaaatcccg caagggtgat gaacaaaaca agccgttgct aaatcaagtt aactatgttc
    11701 tgttgcatct ccatctggca ggcaaacatg acgctaagcc atctcatgct gagctaaagg
    11761 attggcttca cagcggtcaa gtagacgtac ttagaatggc ccccaaaaag taaaacaacc
    11821 gtttcatcgc tctttcctct tctttttaag cacgtcgcca ttctagtgaa tttcattgtc
    11881 atttgcttta tgaagtataa cactgttttg aagttggcat agtgtttaaa tactttcgaa
    11941 tgtatgcaac tctgaactaa gatgccaatt gctataattc cagacgttga tgaacaaaga
    12001 tgtattggtt gtgctctatg tgtagaaatt tgcactgctc tcggccctga tgttctaaga
    12061 gtaaagcctg tagaaggatg gaagagaggt aaggcgttca ttttctaccc agaaaggtgc
    12121 atttcagatg gagcatgtat aggagtatgt ccaacacacg caatcttctg gatgagacca
    12181 atggaatata ccgcaggaca gccagtgcca ctgcacaaaa atggtgtatt cgataaaggc
    12241 tgggaagagg gttaaacgac tgcagcaaat agaagacaaa ccacaaaacg actacaaaag
    12301 acaaaatatt actcttcttt tttaaatttt ttatattgat atataattac acgtgttgag
    12361 gctgtgccag caaagattta actacttctg tccacgcaca ggcgatattt gctcgattat
    12421 atcctcctcc accgagagcg atcagcctac ccatgcagtg tttatgagcc agtccatgta
    12481 ttacgtctgt tgcatatctg tgtgcgttgg gggtatattt taaatgggct agagggtcgc
    12541 cattaagtga atctgcacca cattgtaata taatcaattc aggtttggct acattattta
    12601 taaaattctc aattttgtta aaggctataa tgaaatcttt atcaattgag tttggcgcca
    12661 gcggtatatt cagtttagtg ccttcggcct caccgatacc ggtttctagt tctgagccgg
    12721 ttccaggata taggaagcga ccatcttcat gtatatctgc catgaagagt agtgggtcgt
    12781 tttcgaattc ataataaatc ccatcgccgt gatgagcatc aatatctacg tacgctattc
    12841 gctttagacc atatcgtttt cttgctatta ggattgctac acctatatca ttaaagacac
    12901 aaaatccagc agcagaatcc cttcgtgcgt gatgcagtcc cccgatcgga ttaaagacat
    12961 gttgtacatc atgcggtttt ttcattaaca aatttaaaca ttctagggtc gtaccaacga
    13021 caaaagaaga cgcttcgaaa ataccttgaa atgctggggt gtctcccatg tcaagaaagc
    13081 cactcccagt ctttgagctt ttcttgacaa gatcaacata atccctttca tggaaagtaa
    13141 gtattgattc ttcatcagcc gaaacaggat cctttattac gatttgacta gatttgtcca
    13201 aattctcatt ttgaaatttt gaccagaagg caaagattct atcatggcgc cacggatgcg
    13261 gattcccaaa cccatatctt gctaatgcgt ctccataaaa tacggcggtc cgacacatgc
    13321 atattaactg aataccccat attataatta gcttgatatt tggttgatcg ttgtaccatt
    13381 tgcccaaact taaggggggt tgagcaacta tatctcatac atctgatggc agtaactgat
    13441 gtcaataata taccgaaagt ctatgttcca caaacattta ggtccataaa tgggctctga
    13501 gcgtagtgtg cgtgttcctt gttcataatc tggacaaagg tgtttaagac ttgttagccc
    13561 acatgaaata ggtgaaaata atctcataga catcaatcta tattctaaaa aaaattgagc
    13621 atttaagaaa aatattatag aacccattcg atcttttaat ttggaaccag tacagttgaa
    13681 cgaaacccga gtatgcgaag gacaaatgaa ttatgactag agtggtcatt acaggaaatc
    13741 ctggagttgg aaagcacacg tgtgcaaaat tcgtatccga attgttaggg aaggcaaaag
    13801 taatcgacat taacagggtc atactgagta acaacgcggt aaccttacct cgtagcgcgg
    13861 tgactggaat cggagaaata gacgttaaaa aaacagagaa gcttataata gaagaaataa
    13921 aaaaaacaaa agatgtcgta atagtaggac atctcgcacc gtatgtgtta aggacaactg
    13981 gaattgacct tgttgcagtt ttgaggcgct caccctacca gttagcaaag atttttcggc
    14041 agagaaaata tacacctatg aagatgaaag agaatattgc cgccgagatc cttggcatca
    14101 ctctttacga ctctgtggaa acatttggaa aagaaagggt tgccgaattg gatacgacgg
    14161 gaaaaactcc acaagatatt gccaatgata tcgtttcaaa attacagaag tctagaaaac
    14221 aaatagggat cattgattgg ctttctctag tatatgaaaa aggagatgct caaaagttcc
    14281 tcgagtacta ggtaatttag actgagatag attggtcaag ttgactctcg aacctatctt
    14341 acttacctat ggaaattttt attaatcaac aacaaaaatt catgtatata ttaccccagc
    14401 gctccaccat gaaatattat gagtgcagtg cttactcgaa aatttgggga agagacggtt
    14461 caatttctgg gtaaaaaggt gtcggtggaa acttccgatc aaaaaactta caatggaacc
    14521 ttaactggca tcgacgagaa actcgatgtg gttttggaga atgtggagag tcttggaatc
    14581 attaagatga ttattaacgg ttcttttgta aaagagataa aactaatgga aaagccattt
    14641 gacttcaagg ctctttcgga tagaattgcc agagtttttc ctggtttagt aaagataagg
    14701 gaagacgtag gagctataat tgtgatggac aaaattaaag tcacacaagc aggcatcgag
    14761 gaagggtctg ggcttgcagc agacagagtt aaggctattt atgaagaatt tattagggag
    14821 ataaagaagt aaattttgtc ctttgaagtc aggtatagtg acctggctgg gcgcattgga
    14881 aagctggaga ctccccatgg tatccttgaa actccggctt ttattcctgt tgtccatcct
    14941 gtaaggcaga caattagtcc ccaatttcta aaaaatttag gttttaacgg actgatcacc
    15001 aatgcgtata cgacactaaa atattatggt gacgatgcgc gaactagagg gattcatgat
    15061 attctaaatt atgatggtgt tataatgact gactctgggg ggtatcaaat tttagagtac
    15121 ggagccatca aagccgaacc cggagttata tcccaatttg aaagagatat cggttctgat
    15181 atctctgttc ctctggacaa accaactggt tatggattaa aatatcaaaa agcgaaagac
    15241 cacgtagaag agacactcgt gaatgcaaag gatactcttg aaggcagaaa cgttggaaga
    15301 aaagatagcg aacaaggcta tgataatcac aattgtacga tctgggtagg accggtgcaa
    15361 ggcggcgagc atctagacct ggttagatat tgtgcaaacg ctctggataa aatgggattc
    15421 ccggtattag ctataggtag ccctgtagag gtaatggagg catatgagtt ctcaatattg
    15481 gcgcaaatga tcgcggctgt taaacgcaca gtgcctacaa agcccattca cctgttcgga
    15541 gctggtcatc cgctcactat tcctctggct gtctcgttgg gatgcgatat gtttgattcg
    15601 gcttcataca tgttatatgc caaagatgat cgctatatgc atgcaaatgg cacactcaaa
    15661 ctccaagacc tttcatatct tacttgtcaa tgtccggttt gctgtaccca caccatcaag
    15721 gagcttcgac aaatgagtag agttgatcgc acagaagaaa ttgctaagca taatctatac
    15781 gtcttgaaag cagaagttgg cacagtcaaa caagcgatag tcgatggcag attgtgggaa
    15841 tatgtgatgc ttaaggcacg cgctcatccc aagcttatga aagcaatgga tctttttaaa
    15901 aattttgaat tcttggaaga tggaacaccg ctgtttaaag gcaaggcggt ctttctttat
    15961 gaaccgatag atcaatacag accagaagct aggaggttca gaagaatcgt ctctacgttt
    16021 agatcagtag tcaaaaagaa cttagtttta tatccaataa tgcaactaca gccattttac
    16081 actacaaggg attttgttca gcttgtaaaa aaattccccg atgcacagat ttgtgcatat
    16141 aatcaattcc ttggggttat tccagtagaa atatgtgata tcttccctgc agctcaccat
    16201 ctgagttcgg cgactgcagc aacatgtcac caagccaagg attatccaac gttcattgaa
    16261 tcccttgacg ccttcttggc atgcaatact tttggagaca taactatagt tgcagatgat
    16321 tttatgcatg acctcatata caatagtacg tacaaagcca aattaaatgc aaaagttttg
    16381 gactataaag aaggaattgt ttttgaattg taatcatgtc ttaataagga cgttccagca
    16441 gagcggttac gctggataaa taatcatcca cgtaactacc atgttcgtta tcacatcatg
    16501 cacccatcag ccgatccatc gagctcgttc attcaacttg aatttggaca gctctgccga
    16561 aagggatatc ctccagagga cattaaaaaa tcagaccaag gtaatgatgc atgctcactg
    16621 tctacgaggc agagctaaag ggaaagatag gaagacttac aactgacttg caatacttgg
    16681 cagtcgaact ttaaatttat acaattctat tcttaacatt tccttgtact gcttcgatcc
    16741 tccgaccaaa ccacaagttg gcattacctt cagaagattt aatttgtacc aggagcatcc
    16801 ctgtcgtctt ctcaattttt gtcaaagcac tagtatcgag ataataatcg tcagcaccga
    16861 atattagaaa ttcccagcct tctcctgatg catatttata attaaaattt tttttaaatt
    16921 cgacagcatg taatatccat tctatgcatt catctatttg tagttgcgtt agttagctca
    16981 ctttttcttt acttgtaacg atatttttta aggggttggg tcatattaac cagaaaagta
    17041 agagaaaagt tagaatgtaa caaatcaact tgattgcctc agactaaaaa tgtagcagaa
    17101 ggaaagtgaa ttggcgcaat gagtatcgac ttcgacataa tctgccttag ctcaatcacc
    17161 tgcctgttta ctagacgtct gagacgcggc ttttattata atggttctaa taatatcatg
    17221 aatacattgc ccccgaatgt aagaagatag aggcgcattg aattaaaaat gcttataagg
    17281 atgcgataaa acgctttcac ctaaaaaggc attccgagga tatgatgatt atttttaaac
    17341 tcgtatctta ttgttattat tattttttac caaatcgcgc ttactctaac agtagaaaaa
    17401 ggtatattga tttaagaaaa tacaaaatgt aacagtggtt agaagctcaa aaaaagccgt
    17461 ttatgcagct ctttttggta atcttggtat agctatagcc aaactcatcg cggctattct
    17521 caccggcagc acagcaatgt tcgcagaaac gctgcactct atttcggata catttaacca
    17581 agtactgctt ctattcggaa tcaaaaccag cactaaggct gctactgaac aacatccttt
    17641 cggatacgga aaagagcaat tcttttggtc attcgttgta gcaaccatgc tatttggaat
    17701 atctggcatc ttgtcgttac aacaaggttt tggttcatta ttgagtagga ttcatcatat
    17761 ccagaacgct gacgtgagct atgtcatttt ggcgatatct gctgcttttg aagcgaatgc
    17821 attaagagtt gcattgctcc tagctaaaga gtctattgaa gcacgcggag agaagataag
    17881 tcttaatacg ctagtgcagg aattccggga aagcaaagat ccttctgtta ttacagttat
    17941 ggttgaagat tcggcagcat tggttgggat cgttgttgca ggcataggta tatttttttc
    18001 tgaggcaact ggagatacaa tctacgattc tttaagttca gttgctatag gaggtatttt
    18061 gatggctttt gcttactttc tagctaaaga gaacaaggca ctactaatag gtgagtcgat
    18121 atcaaggaag gattacaata agatcgtgaa actcgtcaat gagatgccag atgtcaacag
    18181 aataataacc atgcgtacca tgcacttcgc tcctgaagat gtgttagtta ctattgaagt
    18241 aaatcttgtt gatggtttgg atacggacaa aatcgaatct gtaatagata atatagaaca
    18301 gaaggtaaaa cagactatcc catatattaa tccagccaaa gtttatgtgg aagtagaaca
    18361 agacaaactc tctgattcct acaggaaaaa gaggtaagaa aaaggaataa cgagcacggg
    18421 taacttaaaa tttcatagca gcctggaata ttagattcgt aatgcggtta cgtatgggat
    18481 acggtccaca tcaatctcag ataggttatg tgaattgctg agctcgtatg ctgtatcttc
    18541 tgcatgtttt aaattgggcg agcctagaca tcacgatgtt atagtgcacc cataattcat
    18601 ctaaagcttt agtcaatcat taataattct atgcaaccat cgctattgaa gtctacaaaa
    18661 atgaagagat atgaatgact catggagaac taggaataaa tccctctttc taattctact
    18721 agagtacgaa taaatagctt attcgtaatc atttcaaacc actcgcttat gtctgggcca
    18781 gacgatttaa tgcaggcaaa aatagttcat ctgattctta acaagaaaac cgaggaagca
    18841 ctagaaaaat taagcgattt ttatcatgtt gacactcctg aaatagtagt tggtacaatc
    18901 aaaggaaaga gaagaactgt atacgcagtg tatgttcaaa aagaaagaaa aatctacgca
    18961 ttaaattctg acatcttcta caatcctttc gtggtgttac acgaatatta tcatcatata
    19021 agatcaaagg gtggcacgca ccgggggtcc gaaaagcgtg ccaatttgta cgctcaacac
    19081 ttcataatgg cgtataaaaa ggtaatggga agtaactaga aacgcaccca gactgcctac
    19141 gtagggtaga tgtgtaacag tagttataga ttaatcgtta caaatatgcc gttccaaaag
    19201 ttctatgacc tatacattat gcactgcatc ggataacaat ctcttttcct ggtattcaga
    19261 gggtttcaaa actgcatagt acggagcttg tctacgtaca tgttctacat gcaatcgaat
    19321 agagagcaat gactatgtgt gtagaaagaa aaggtcatat gcgaatgccc tatgaaatac
    19381 aaaaggtagt agcaataatc agcagcaatt ttataagacc gtaggatcaa ataatagctg
    19441 acgagtaatt gagtactaac aacaaaaagt ttcttattaa ccaaataatg tggttaggcc
    19501 tatcccttgg tatcagcctc gccatatctt tcatcgtacc ctttccttat tcgttggttg
    19561 ttataatagg tgttttcatt ctgctcaact tctacatgag aaagagaatg atgggaagaa
    19621 tgggtggcgg gatgtttggt tcgatgtcct cttcttcaac cttcggtgat aattcgctga
    19681 aatactactg tatgggctgc ggaacgaaac ataaagaggc tgcgtgtcca aaatgtggct
    19741 ctaagatgaa aagagtaggc agttgagata aacgaccgtc ataaacaact aaaaacaagt
    19801 gctcattagt agagctacga tgataaatat ccatgggtaa tatgtctgct tgacagctac
    19861 tttaactccg aatgttggtt tcacctacag taatattatg cagtactatt acgcgtatgg
    19921 gaagaagtag gattgactat cactgcagaa tcagacatcc tcgtattgct tactacaaca
    19981 ttgttgtcaa ccgcatcgat agctgtggcg atattaataa ggcgcaccgc cattggtgat
    20041 ggcaatacaa aatgttactt agcatacata aaagttgctt ttcaccacca cctaaatcct
    20101 ttctttgcct aacaaaacca ataagcatgg cagaagaaaa tatcggttgc tgcaacaaat
    20161 aattacggta tgagaggaat ttacaattta gtttgcaaca tgaattttat caataaagaa
    20221 tggtttttaa cattactggt attttttatt ggttgtgtgg tccgtctcgt accagagtta
    20281 tttgcatatc cttctccaat tggttatgat gtgatcaact actatattcc cgtgacaacc
    20341 aattttacta tccactggac tcaaatttca tcagagttcc ctttttacgt ttcttttctt
    20401 catgtgatac agattgcaac tggccttacg ccctattcta ctgtcgtaat ttttgctact
    20461 gtgatctttg gagtttttac tgtttcagta tttttgatcg ctcggaaatt gctgaaagtt
    20521 gatattagat atgctttttt tgtcacagtc tttgtaattt ttcaaatggc ggtacttcga
    20581 acaacatggg atcttcatcg agatgttttg gcgcttacta tgatgttctt tgtgtttgta
    20641 ttaatctaca gagaatccga aagaaagagg actaaaaaat taaattggaa atttattctt
    20701 ctcgctctga tcctctgtgt attggttgtc tcgactgcta gaatagttgg gtcgttattc
    20761 gtcgtctctc ttataattta ttcgtctctc gttaagacta gagctgtgat tttgtgtacc
    20821 atcgttgcgt ttagcttctt tacaattgaa ttgttcatga atcagaatat tataagtaat
    20881 ataattcatg aaaacataca gaaaacaggg aatggttcat cagttcattc tacgttttat
    20941 aatccaaaaa atttattgta cttatttgta gttgtagatg gcctacttgt tccaacaggt
    21001 ataattggat ttaaacaatt gaagaatggc actttgctta aaattcctgt actgataact
    21061 gctgctgctt cattttcatg gattgttttt ccttttgatg agttgttagt tgcagatcga
    21121 tggattagtt tgctgggtat ttttctttca atttttgctg catttggtct ctatcaatta
    21181 attctgacga tgaaaataag aggacctgca catagagctt ctacttctgc tactaatact
    21241 cccactctct ctaatcctgc ttccacgata ctcacttctt ttattctagg aatattcgtt
    21301 ataatgggga tattatacga gatagtgcca aacgctcatg aaccatttac gttgtggtat
    21361 ggaatggcac gtagctatat agagcatttt gttcctccta gtatgcaatt taattctctc
    21421 cagctaagtg atacttacaa gatgatgtct gcaatttcat ggattaataa aaatacccaa
    21481 ccaaatgcca ttattattgg cgaaaaacat tggcgtggct ttatggagtt gtatctgcag
    21541 gatcaccgta cttttcgctt ttccgatgat ttaccaacgt tgacaaccgg attaatcaaa
    21601 catggactca atgcaccaat ttatataatt cattatccgg ataatcgaag caaaaacacg
    21661 gatgtctatt ccaatattct attttctata gacaagatca aatagtctcc ccataggatt
    21721 agctgaatct caaatttgtg ttagtccgcc tgcttctatt ctattacaca tgcttatcaa
    21781 tacataccca atgtgtgaat caaaattttc gcgttgaact gaatccagat attctgctag
    21841 accgcggcag ttaagagtgg ctattcctat gtagcttgca atttgtattg tatttttatt
    21901 gtgcatgttc tattacgctt taaaaaatcg cacacggcat ctatgtcgtg atatggcagt
    21961 ctttatttag aaatcttttt catgtgcttt ggagtaagta gccaactaag ctcgccatga
    22021 atcatttgat gggaggagga agttattcta acctttgcca ggccagcttt cttcagaact
    22081 accagtccac cagcagtacc cacacgattt ccctcagata taacctcacc tatcagggta
    22141 ctaagataag gttcatgtcc tacaatcagt atagaggaat cttgtttgaa tttttgggac
    22201 aatttacgat aaagctcttt tctattgcct tccggtttta actcatccca gttctccata
    22261 ttgttctccc tgttaaaggt cgtggccaca agggaagcag tttggtgtga tctctttaga
    22321 gggcttgtga ttatgaagtt aaactggaca cctaactcct ttagcgattt tgctatatct
    22381 gtcacttctc ttttacctgt aaccgtgagt ggtctaccaa agtcgctgat accagaagag
    22441 agtcttttgc cagcctcgcc gtggcgtaga atgaataagt ccatgttcga ggaatagatc
    22501 ttgtacgagt aataactatt ttctcaaaag agagtgagta gagtcgatct tcgactcatt
    22561 tgctaatgca ccggcaaaaa ttggttccct gtatgtatct ctatacctat agtacttaca
    22621 ctccgaaaac tttgcgttta acatctaatt gattcgttct attctaccgt ccattaaacc
    22681 ttcgagccct cagtggaact gattttccgt gaattctaaa ttactatctg cattcttggt
    22741 catgtatctc tacacaaatt tatttcagcc catagataga ctaattatcg ctatcgtcga
    22801 cgctcattat gttaatttaa tggttcagcc tataatttta ttgcactaga ggattgatgt
    22861 atgcgcaatc agagctcttc caatccttta gcgctgcggc tcgtcgtcgt tatcttggga
    22921 aaattgacct cttctagttt aatggtttaa cacctctggt atcttgtgat tctcgtcatc
    22981 tgcgtttaga tagaacaact gtgtgtgcat aattatgcta ataccaaact ttactaccgc
    23041 agtagcaact tgaagtaagg gagtcaactt agttaaccaa tatattatat ataatcacaa
    23101 aatcgtaaaa gacacaaagg tttcatctta tcttgtacag cacactaaaa ccagcaagca
    23161 ttgtcagact cttgtcacca ttatgttttg tatttacaat cgtaatgttt gtattcgtat
    23221 cagtttattc cttctcgtcg cttattacta agtcatcaac tattccttat cagaaggcat
    23281 tggctctggc ggctaaggcc atggctagag cgcaggctcc atcttccctg acaccatctt
    23341 ctatgacaca gaatttcaca acctatacag acctagctga cggcatatcg attaaatatc
    23401 cgtctggttg ggaaaaaata gaatatcctg cagtgccgat gtcaacaacc ggctacagag
    23461 ttgtcgttaa tttcctggca cctatagtaa atgcatcgga tcagtggcga gaatatttaa
    23521 tgttaaaagt gctaaaccaa agcaccgtca agagtctcgt cccccaggtg aacactactc
    23581 ttgcagggaa tcccgggtac aaactggttt ataccaataa cgaagaatca tttcatctta
    23641 aaacacttga agtttggacg acaattggcg atcatacata tttgcttatt tataaagccg
    23701 aagcaacaaa atactctagt tatctgccaa taatacaaag gatgctagat tcattcagag
    23761 taggggtttc accgggctaa ccagttctat tacgactacg actactacta ctaaattact
    23821 ttctaaataa atactctgta aaatcagctt tcatatttgc tacgtggaca agactaggtt
    23881 aatctccact atcactagta ttgcccctac tgaccgtcta catcggttac tagccgtttg
    23941 gtcttatatc gaatttatgc tacctcgtag ggaacatttt tctaagatcg ttgtgaagac
    24001 catataatat cctcttcctc ttacctagtc ttattaattg ctgcttttct tggaaatttt
    24061 cttgggtttt ataatggctt tatggtaatc atcgcctatt tagactgacc aaagtgacga
    24121 atcaggactg ccatctatag atatcattta gaacccggtt tgcgttacta atctgaaata
    24181 agttctataa cattagcgaa aatctactag aaaacccata tatagttatg tgctgtaggc
    24241 agtacaatgc acaaacaaac atcattccta ataacggcag caatattgat agccgcagtt
    24301 gcgacagtgg cgctttacac ggtcaaggca gcgattgcac aacccacgat gacaaagggc
    24361 cctacgaata cgactgcagg cggcgcggca ggtaannnnn nnnnnnnnnn nnnnnnnnnn
    24421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng caggtaatgc
    24601 gacaaaagca ggtaacatga ctgctggcgg cggtaacatg acaaagtcta agtaactttg
    24661 ctcgaagcca ttatcgtcgt gttgattgca gcgtagaaaa cgtaccggag tcaacacgtt
    24721 ttttttagta acaaacctga atccatatgt aactacgttt tgcgtataca agatttggct
    24781 tcttgtatac tcagtccttt aaaaattcag accatgaaat cttaacattt ctcattatat
    24841 agaccgacgc atcgtatgtc taaaacgcgt agatagaaag agcatcagcc acacgtgtat
    24901 ctgtgtgagt actccttacg ggctttattc atactataat aaaatgtggc agctcttggc
    24961 cctaagccct tgcttatagc aaagttatgt ttcaatgatg gatctgtcag tcacaagtcc
    25021 agacaataaa atacggaaat ttaattctag atttaggaag tggactatta ttgtcggtta
    25081 taaggaatat tcctaggcct tgtcataaaa tttatgttag tcccacttat tactttgaac
    25141 gttaaactta ctagtctgta gccttcaagt tgacgctcta tatggaatca cacaagtatt
    25201 gttagattta aactacgtgc cgagcatgtt agagcattat ttgctcgact tcccttaatg
    25261 aatttactct gagaggccaa ttaaaatctt tgttccaagg ctggttgaat aatattccga
    25321 cctttggagg ggttatctcg actaaattgc ttggagcatc gtcaattagg atatcaaagg
    25381 gatattctgc tttaggtctg ttgtcatata tgaaaatgag atcgtcagaa taaatatcat
    25441 gataatcaag ccatttcgta acataagcta tggtgggtct ctctctttta gttaaaatag
    25501 aaatcctata acctctacga cggattcttt ttatgatctc actttgtcct ggctcgctag
    25561 gtggaatatt tcgccagcaa tgttgccaaa cgtagttaaa cattgtagaa atttcatcag
    25621 ctgagagggg cagaatcatt ccaatattcc agtatgttat ctcgcttttg gttatatttg
    25681 tatttttgag cttgttatac tccttggtcc atactatcat agtgtcagca aggactgaat
    25741 caaaatctag tgataaagtc ttagttgctc cgaaagcaga cattgattaa acaatcacag
    25801 tttactaagg atgataattt atcttttcgt tgagtttgat actgttgcgt ctgcgaccaa
    25861 ggaaaaaaaa atggaggctc taattatagc aatctattta tcgacaggtg ttctgacgtt
    25921 tgcgaagacc tagatgcctt ttctttctgc ttacgcaggt ccaatctgat tacctgatga
    25981 ccgtgcgcga agtcaggacg aaatagatat ggaaaaatta attgaccggg attgtcatct
    26041 tttttcccaa gaaatgaatt ccatactttt ggtacgcctt gctgatccta gtatccaata
    26101 ctgatctctg tcagtcttca tcgtcggtgg cgggcggcac tctaataata gcagctaatt
    26161 gacaagtcct atcacgtcta ctcttgagaa tagcaatctt attatcccaa attcgtcgtc
    26221 tgtaaatgcg agcgaagtta gcccagcctg gttaaggtac gagcttccca agctcgggat
    26281 cgcgggttca aatcccgcac ttcgcatgtt ttttttcaag gtgttacggt cactgaacgg
    26341 tactacctat cttactacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn ntggtactac ctatcttact actatacaat aatttttctc cgttgcttgg
    26581 cccactaatc aatgctttta taaaaaggca ctgtcgtaaa tcgcagatac gcaaatcatt
    26641 aatgaaagtt tgacgatcct gcgatagcgc gggctttatc ttagcagtag ccaaggagaa
    26701 taatagtatg gttttgattg aagagggcta tgattataat cgataatggt atgacggata
    26761 ctgttattaa attggtatct aattaatata agattgtctg aaatgaaaga atttcataca
    26821 gattacttag gaaagtcata taactttatt tttcttacga ggcaggcata gcaggaagag
    26881 atgcctacgc tgttagaagc ccctgatcaa aattttgttg gaacgagata attcgttgat
    26941 gataataatg ataacgacta ggatatgtgc tcaagttaaa agaaaggtaa tacataaacg
    27001 gctaaatgcc ggtgggtgca actaaatcac caacttggtt atagatttgc aagaagtaca
    27061 tccctttgta tgttgtctta aaggtttgct tctcttcctc gtaaactaac atacgatttt
    27121 cttgtaacgc cgtcaaataa ttctgcaatt gatcaggaga taggaagcga ttaagtagaa
    27181 ttcgacccct tttgaagttg agtgtctcga gaatcattgc aattatgtca agcggagtgt
    27241 attccaatta tgtccaaagc cattgggaaa ctaagtattt atataaaact aatatttgta
    27301 ggcgtctatt tacattaaaa caaatcacgt atgacttaga ttaaatatag tttagtagta
    27361 acccttttct tatccgaacg tgatcaataa tatattgtgc tactactgtt atcttctctg
    27421 taaatttgtt cacttatttg gaacaattat ttattctcat gctgctcccc gcagcattcg
    27481 gcttttccgg gtggctagaa gcgatctcag acacttaatt tcattaatgg atcaaaatca
    27541 gattcggtct tactatttat caaactaggt gtatacggga tttcacagta ataatgagtc
    27601 tatctaataa aatgcggatt tttttgtgct agtggctata ctagattgac ccgaatacta
    27661 ctgacaaatt tcgagtcatt tttttaaatc ctccgtatat cgatgttgga gatagcaaat
    27721 gtagttggga cttgatatat cccatattgt ttgtccgttc tctaataacc tagtgcagga
    27781 ttgcagtttg gcaagaagaa gacccatttt taaatagttg aaatctaata tagtcgttga
    27841 aagagtttgt tatcaccaaa ggggcccgac atggaatgac attttatctt tccaccaatc
    27901 taatgagaca actatctcac atgctgcctc taacccggtc tgcttgctac ctctctggtc
    27961 cacgcttcga ataccatttt aatgatattt ctcagtcatg cacaattggc taaactgtgt
    28021 tgttgcctgt tacaattttc tagttcttga acctgctaga accctaactt tgttctgatg
    28081 gactcctatt tccgcattat ggaaaacacg gattccgtaa ttaatgccta cagccattgt
    28141 ttcaatatat atgaacaaac tttgttcata gactgctaag atatattgtt agttgtgcta
    28201 tctagaaaag aaagtttcac tgatcccaac aattctgata taggcttgat atgcccatag
    28261 ccatccatta gtctgttcca tattcccttt atctttcctc tattctccgg gtcgtctttc
    28321 agcaatacaa ttatgtcttc taataaagca gctagcttcg gtttatcctc ttcttctact
    28381 tcgctcctta cgatttttat ttctgaagac tcgtttatgt cagaacttcg ttgtgataat
    28441 cctcgagtaa ggatgtcgat cctcttaacg atctcatctc gttccataga tgaataatcc
    28501 taaggaatta tataaagtgg aatcattgcc aaagcataaa gaagtatggc ggaggatcag
    28561 agaatcacta ggagtagtat ctttctcctt ttagtcctaa tcctatactt ctactagctt
    28621 caaggctcat aacaaggtcc agattcgact tccttctaag gggcagtcgc aatgatacat
    28681 caatattcat tttattatat aagcagtaaa gtagtaggac cagccgtcat atcgaaccgg
    28741 ggtgttctga tcgggttcgt agtgtgataa ataattaatc tacagcaggt acaagcacat
    28801 acaggagtat ctattaaatt aaagtagcct ctcattcttg gttagagaaa ggatgaacga
    28861 taaattggtc ttactaactg gtgcactcgc gatagctatg gtcacaggga tattattggt
    28921 agttgttagt gctagaattt tgggaccaga attcgtttct gggaaattaa tttctgcaag
    28981 gctcttgact aatgctaaca ccctgaataa tctcacaaca gggaatgtgg tcaaagtaaa
    29041 gactggcggg ggagacaata atgtgtcatc aacccaattt ttcccacaaa cagtcgaaat
    29101 taagactgga ggaagtgtta cttggtacaa tcccactcgg gtaccagaac cccataccgt
    29161 tacctttcag atgggtagtt tatacccaac acaatctttt ctgccggtag aggtgtcaaa
    29221 atctgctcaa ttaaccctat tgccgtcggc tgcttctaac ggtgaaccaa ttgttgtccc
    29281 cgggcaaaac cacacaagtt tcataatagc cgcaaatgct agggcataca atccggttgt
    29341 cattaattca aatggcagta tgacattcct taattgggat gcgaaatata ctatgagtgg
    29401 aagtgagaag tatattaatt cgggctggtt gttcccaaac ggcgagatcc cgccaggcat
    29461 tcaagctgga agaacgttca cgataacttt cgagaaagca ggcttatatg agtacacttg
    29521 tgtttttcat ccatggatga ctggaaaggt cattgtaaaa taaataatct aaattaccat
    29581 ataatagtac taatagatcc gttgcattga tcgcgtgaat gtgattttat agaaaattac
    29641 cctcccagtc aattatacgg tattattttt aacgactatg gtttggtata tttatcaatc
    29701 ggtatgatga gacgaaagta atcatagcca acgcgaatag aaaaacacct gttgataaat
    29761 gggttgccac aagcaaagcg tttaattttg tattgacaac aaacataccc aatacaatct
    29821 gaactgcgac tataatacca gctgtaatag cagtttttct cataatacct gatttcttcg
    29881 cagcataacc tgcagtagca aagataacgc ctgcagtcat cgccgcaaca attctatgga
    29941 tttcctcaaa aaggtacttt tttggtggaa aattgaaccc gttgggacaa agtggccaat
    30001 cagggcatga tagcccttga tgtgaggcat caacataagt tccgattagc atcaccgtaa
    30061 agagcaaagc taatgacgcg aaagaaaggc aaactatcct catcacaatc atgcaatcat
    30121 ccaccatggt acgcgctaat agataattat taaccttaca tcttgcttcg aagagtggct
    30181 gacatcgccg catactacga aaggctcttc caaaaatgac tgcgatacaa tgaaccttat
    30241 atcaatagct agtacttacg gaagaggctt tgaagctgag aaagttgaaa aataccaagt
    30301 atcagtcccg actaacctat taaaataaga atccttaaca tataattgaa attgatacgg
    30361 caaatagcag ataatcgaac gatcaagttt atcatctgct agcccagatt tgtcatgaca
    30421 gcgtgttagc taaggtaatg aagcaaaaga aagttttatt attatcgatg ctaacttagt
    30481 tgtaacctgt tcagttcttt ctaacattcg tcggatattc cgatcatgca gagaaatttt
    30541 tctagtttat ttcattacgg ctaagtcatg ccgatgcgga tgggctagcg tcggagctat
    30601 tgaccttcat caacaaatat tctagcttct gctgcaacat tgaattaatt ttatggccgt
    30661 ctgctcttcc tctaagcagt gccatgcttt tacccatgag tattccaact gccccgagtc
    30721 gtttttcaat aattatagac atattctctt ttagaatctt ctcaatagct gtgtccaatt
    30781 cttgatcact tatcgctgtt atgccgagtg ccattttggc ttcttcgact gtttcagcct
    30841 cttttttcat cagtttttcg aaaattgaga taacagattc tttagcgatc gtgccattat
    30901 caagtctttt gaaaacgtca ataaggattt catccgttat aactgctgga tccaatccgt
    30961 gtcgttccag attaattagg tcttctgtaa gtttcgatgc aataaatgtt ggacgtacct
    31021 ctttcaccga cccggcaatt tcctcaaaaa tgccgaaata atcagaatca aagatttgct
    31081 ctgcaagttt tttgttcagg ctgtatttct tggccaggga atttaagatt tcgtccattg
    31141 atttggggac attttcagaa agggatgcta ttcttgaact gttgattgga atggacggaa
    31201 cgtcagtttc gggatacatc cttgccgcgc ccggtcttgg tctactgaat actgtttttc
    31261 cctcaaacgt agcagcgcgt gtttctggcg gaacaccatt caacgccatc tttaatctct
    31321 gaattatggc ttctattgca agatcgacct taaagttttg accaccaacg agtacgaatg
    31381 catccatttc ggcatccata cgcaatttat tttttattgc ctgaacttct tcgctgctga
    31441 taccatacct tggtaactca tcagaatgaa atacgcctcc cagtccgtaa aatctaacca
    31501 actcacttag ttgctttcct atacgtatat ccggatatgg ctcaaatcct atcatacccc
    31561 caaaacccct taccctgatt gccttgaaaa tgctattatt ttcttggagt gaatttatca
    31621 ccactttcga aggagacttc attaaaatgt cggttacatc ttcaatcctg tcaccaattt
    31681 ctatttcaac atctttccta tctcttagtt tttgggcaat tgtaatcaaa ccatgctgtc
    31741 tgagcatttc atattgtatt acttttacaa gctggtccaa tcgctgcaca ccctttactt
    31801 caaccactgc tccgttatct atggagatat taacatcctg tcttatgctt ccaagccctc
    31861 gtgccactct cttacttgcg cgtaatagcc tgccgaggga cagtgcaatc cgcattattt
    31921 cctcaggcgt gccagtcacc ggctcgagtg caatttcaac tagtggcacc cctaatctgt
    31981 ccagtccgta ctccttaatt gtaccgtcgt ctgaaatcaa tttggccgca tcttcttcaa
    32041 gacatatact ttgcactttc actctttttt cattaacgtc aaggtaccca ccattggcaa
    32101 ctagaattgt tcgctgaaac ccgcctgtat ttgagccatc aattacgaat ttacgcataa
    32161 catgcaactc atcgccaata tccgaatgaa gggctaggga aattatcagt gcagtttcga
    32221 gcgagccgtt gtcaacgtcg tgtggtggtt cctcatcggc ctctactaag caactagaag
    32281 ttactgatgc gtaatacttt atcgtacgca tctttttgaa ttcgaacagt gcagcaggat
    32341 catacgagcc caattcgctc tgtgttggtc gcaatttacg aacgaaggtc gcgccatact
    32401 tttcaatctc tttagatcta caactacaaa atagtttgcc gctggttgct aactgctggt
    32461 gaatttcaat cccgactttg agattaatga ctactggatc tatcgtcaaa gttacaagtc
    32521 caatttggac tggacatctt ttaagcttac ctatcagata ggtatagaca tcgatatttc
    32581 atttgcaata ttttcctgca tcaatttcgt ggcagattta gcgtctttac tgtttgcgag
    32641 ggcccacatt gcttttacgg tagcagtctc tgccatcatg tctgataacg ggattacgcc
    32701 aatatccagc agatccctac cagtctcgta tacagtcatt ctcgtccttc cccagataca
    32761 ttgcgaagtc atgaagatca gtgtgcctga ttctactgca tttcggattt gtggaaagca
    32821 ctctttacta acatgcccaa ggcctgttcc ttcaagaata atagccttgt aacctgctgc
    32881 caccgcatga gatatgaact ccggattaaa tccaggatag tattttagta gtacagctct
    32941 tctgtcaaag tccggtcgaa ctacaaggcc attagtatta ttattacgct tcttaagttt
    33001 cagccctgtt tgttccaatt gcatttcaag aacttcacct ctgattagag ctataggcac
    33061 gccaactatt gattcaaaag catcacgcct gctggtatga ttctttctca ccctggtacc
    33121 aagatgacaa gcaattacgt catccgaagt ggtagcatgc atgctaacaa aaactccagt
    33181 tagttcagac tttacagcaa atatcgtcgc ccctataaga ttcagggctg cgtctgatga
    33241 gggtctatca gacgatcgct gtgcacctac taatacaaca ggaataggca agttttgtaa
    33301 ggcaaaacta agtgcggctg cagtatagtg catggtgtcg gttccatggg aaactattat
    33361 cccttgatac ttccccgacg tgaccttttc ctttatctta tcagcaataa tggtccagtc
    33421 cgcaggcttc agattttcgc tgtattcatt taacaacacc tcgggctcga ttgaggcata
    33481 attttcaagt tcaggtacag aagcatatat ctcggatgcc gaaagagctg cactgacacc
    33541 tcctgtccga taatctactt tacttgcgat cgtgccacca gtccctagca gcgcaatttt
    33601 gggatgttct ttgctcctgt cattctggtt ggttgattga ttgattttga attccttgct
    33661 tacttgttta aacgaatcta caaaagtcat aattttcgta gcgggcgtat caggtacatc
    33721 tcgagttggg tctgttaatt tagcaatcga tcgaatcttc tctgttccaa tccctaggtt
    33781 gtaaccgctt cttaatttga taacaatatg atttttgtcc gctgattcat aacgcggcat
    33841 caagatgcct gtaaattcat tctcagaggt ctttatcgta atagtgtcgc ctacggcaac
    33901 gtgttgtttc cgaagcaact ccgcgctctt ttctttgtat cctgttaaat tgtccttatc
    33961 tctgatggac aatcctactt ccgtgggatc ttttttctat atattaacaa aactgtggca
    34021 aatagtccct ctacctcgct ttagaggtgc agattcaaac gagagaagga tctaaaagtg
    34081 ctcggtggtt ggtatcaacg tgaacgttcg ataatcgcta tgcttcttgc ctctagaata
    34141 tccagaacgg ccttctcaag atcgtatttt gttatttgtc cgaaatccca tgcgtcaatg
    34201 aatctcctgt actcttgttc tagtattttt gcagtttctt gaaattctac cattttctca
    34261 gggctatcgg gtgctttata gcccttggca atcataatag gacacctttt gtcaccttcg
    34321 agtggaatat accaagcatc ttttgcaaat tcataacgtc ccgtgctcag agtgttatac
    34381 atacgcatta gttcatcgtt tgacagcttt tgcaagtcaa ttagccatct ttccttctcc
    34441 attttgaaca aaatcttttc ctcatccatt ctcgtttcgg tacagctagt tccaaacacc
    34501 aattgtactt atacttatgg acagacagct aaaaatacat tttcttcaaa ttaaaacctc
    34561 cagcaacagg tctcttagct tgcatcgaat atttcagtac taaagtttga gagtctaatg
    34621 acttgattcg gaatgcttca taaaaatcgc tttgattctt tcaattccat gtatatcgtc
    34681 gacaaatttc gcagtttcgg tactgactag gtctttccaa tctcttccgc gtaccatcct
    34741 agacctaatt tctgtaccag atagttcgtt ccgtctatga agaccaggtt ggatgacttc
    34801 aatccccctt tctttataca acagacgtgt aaagaaatca tttgaaaaca ccgactgata
    34861 tctaggaacc ataatatcaa gctggtacgt ccaaagagga tggaagctca catcagggac
    34921 aggaatcatc aatattcttt tgatatcaat tccctgttct gcatcaagtg aatcttttat
    34981 catttgaatc cgttcaccgg cagtaaatgg attcctcatt tcgtgactta tctgtgcgct
    35041 acctattaca attacaagct cttcaacctt ctctagagca aacttgatag ttgctatgtg
    35101 tcctaaatga aatggttgaa atctgccgac aaatatacca cgcgacatta tatattcaac
    35161 attatatact catactagac tcgataattt aaagtaattt tgatatctta ggaaaagatc
    35221 aatttatctg acgtgttcta ttcaagaaaa caacgataat aacaatgata atattgtcgt
    35281 tattctgatt attttatgta tgagttgtag agcagaattt ttatctttct atatccacaa
    35341 gaatgacaga tctttgtgct aacaatcgac ggtgcctatg gtgagggcgg aggacaaatt
    35401 ctgcgtagcg ccgtcacctt atctgctata actgggaagt ctatagaggt aatcaatatc
    35461 cgagcgaaac gtcataatcc tggactcaga ccacagcata tgcacgcgac tagggctgta
    35521 gccgagatat tcagcgcgag ggtagagaat cttaaagtcg gtgccgaatg gataagattt
    35581 gttccaaaac ctgacaatta tgaaggaggc gagttaagaa ttgatacggg aaccgcaggt
    35641 agcattccga tgatattaca aacaattatt cccgctgtgg cgttatcagg caaaagccta
    35701 agtgttcagg taactggtgg taccgatgtg aagatgagtc ctactgctga ttatctgcgc
    35761 tttatcatgc acaacgcgta taaaaatata ggaataaatt tcagcctaaa tatcctcaaa
    35821 agaggatact acccaaaggg cggaggagta atacaggcag agattagtcc ctgtaaaaac
    35881 ataagcccat ttgacctctt gaatagacgc aacatagaac ctaaaattgc gagtgtatgt
    35941 tgccaacttc caaaaagcgt tgcagaaaga cagatttctt ctgctctgtt aagattagaa
    36001 aggcgcggag ttcgctgcaa tttttattcg tcctcatttg aatcttcact atcccctggt
    36061 acttcgattt tggtgtactc tgaatctgat tttggcccat atatcggggg tgattcgatt
    36121 ggcgcaagag acaaatcggc cgaaaaagta ggggaagaag ctgtgcagaa ttttattgaa
    36181 agctatgagg tcaacgttcc aattgatcga tttcttgcag atatgctcgt aatcccgcta
    36241 agtctcacaa agggaaaatc gcgatatagg ataggaatag cgactgaaca tctcaagacg
    36301 aatctgcacg ttgtaagcca aatagtagga tgcaaatatc aaattgaacc cgcagaaaaa
    36361 agttacattg ttactattga aggtagtcgt ttgtcagagt agtataggtt ctagtagtag
    36421 cacataatat ggtattaatt gttaatcatg tgtattgccc tccaaacttg aggctctgcc
    36481 tgctcatagg cgaccaagaa tacgaaaagc agtgtgtatc gtgagtggtg gacttgattc
    36541 tgtttgcacc gcggcctatt tgagagaaga aggttacagc gtttacatgc tcacttttgc
    36601 atacggacag cgagcaagat acgaaattca ggcagcaaaa cgtttcgcaa ggattttgaa
    36661 gattgaagat catagaattg ttgatatctc gttcatgaag gagatttatg gaagaacaaa
    36721 cgtcttaaca gacacaaaac aagttttacc ccaacatttc gattatagta tcgttgtccc
    36781 actgagaaat gcagtattca tcacaattgc aagtgcatgg gccatgagca ttggtgcaga
    36841 actcgtggca tatggtgcac atactggcga cgaacgatat ccagattgtc gtgcaagttt
    36901 cataaagtca ataaccaata cgctgaatct gggcgaatcg gacggcataa gatcgggaac
    36961 gagacaaaag ataactgtgt ggtctccagc agttaacggt atagataagt caggtttgtt
    37021 gagtaaaggc tataaacatc taggcgatcg gattttcgat acatggagct gctattccaa
    37081 cgggttaagg gaaaaaggta aaaatcctat tcagtgtggc agatgcgaat cgtgtataaa
    37141 taggaagatt gcaattagca acgcaggtgt agaggataaa acacgttatg ctaacgaacc
    37201 caaatacaat ccaacttagc gaaatattca ctagtatcga aggcgagggc atcttgtttg
    37261 gtacaaagac actctttgtg agaatggctg gatgtcatct caaatgtcga tggtgtgaca
    37321 ctagctatgc tttaccgatg tatagtggta atagctattc acttgaatat gtaaagaagc
    37381 taatcgcaga tcacctaaag ccctttacct acaaagttaa ttttactgga ggtgagccgt
    37441 tagttcaata cgaggcggtc attgatctag ccaaattcgt tagagagaga ggacttagga
    37501 cttacttaga atcagcatgt tatgactccg ataggtttgc aaaactaatt ccgtatatag
    37561 atatttgtaa agtagagctc aagatgcgcg attccaaggt agttgatatg aatcattatg
    37621 acaacttatt gcagaatgaa attaggtgct taaggacgtc tgttaaaaac cacaaaatca
    37681 catacatcaa aattgtagtg actaattcga ctgatattaa agagttcgca ggccttgtag
    37741 aaaatatctt ccaacaagta cgtactgcag acataagtgg gtttataata cagccgagcg
    37801 acaggataga tgaaccaaca acagaacgtc tgttacaatt ttacgatata gtttgtccct
    37861 attacataga ggtaaggatt atacctcagt tacataaaca aataggggct agataataac
    37921 aatgatgcag ccagatgaaa agataaacat agaaaaagta gatacgatac ataaggaaaa
    37981 aatagaaaaa ttgctaagac aactgctgat agagataggc gaaaacccag atcgagaagg
    38041 cctaattggg actccggcac gtatggcgga gatgtacgag gagatatttg ggggatataa
    38101 aataaactca gaactcgatg taagcttcac cgaggctgca gaaacaatta tcgccaaaga
    38161 tatccaattt tacagtatgt gcgaacatca tatgcttcca tttttcggaa agatacacat
    38221 agcatatgtt cctgctggta aagtatttgg aatatcgaaa ttgatccgcc ttgttgaaaa
    38281 atatgcacgt agaatgcaaa ttcaggagag gcttacaaaa gagattgcgg atgaggtaat
    38341 gagaagagga gtgaaaggag ttctggtcat agcagaaggg gaacatctgt gcatgaaaat
    38401 gcgtggtgtt agaaatggaa gcgtaatact gacagaggcg caccgtggta taatggaaca
    38461 gaaggaaatt cgtgaacatg tcctttcact catttatacc tcaaaaccag aattacaggc
    38521 tatgtgaact tcaaaatcga gaatatagta gagtacacag ccatacagtt atttgcatct
    38581 tagcgacaac aaggcttata ataaagtaat tataggtaaa aagaatggcg agtacgatct
    38641 ttagagtaca gtcacacaaa tttagtcaag acaatattca aactacactg gtatgttcga
    38701 agtgtgacga aatattttct aaagaatttc ctctggatct tgaaggtcag actattgaat
    38761 tcaagtgtcc agtgtgtggc gctcctggcg aggccgatct tccctacaag agtgccgaag
    38821 aaagagagga attagaagaa gaattcgaag aggtggagga agaagaaggc gaagaagaga
    38881 ttgaggaaga ttattagcta ttgcttcaaa ataccactac atctgttagt tactagcctt
    38941 tgatcctaac ttgcccaatt gcgccaacaa cgcagctaac tggatttcag gatgggcacc
    39001 cgctgcgagt ctgtaatcat attctgccat tagagctgcg aattcatcag gatgatccaa
    39061 tttcatatca tacgcttcct gattagcata tttgagaaaa tcattttcag acaatccata
    39121 cacgtttgtc aattcaaata gtttgaccct ggcgtcagtg aacctaccag caagagctag
    39181 tctgataact tctccgactt tggatttgcc tgaaagccct cttgatgcaa ttacattggc
    39241 gtgtgttacg gaccccatgc cagccgacgc ctggagtatg ttaatagagt ggcgtaagtc
    39301 tcccgaagtg gcatcataga tctgtgagag tgctttttct tcaaatttag ttccttcttt
    39361 ttcacagatc gtttttaggt gtcgtacgag gtcttccttc gccaatcttg taaaccgaaa
    39421 gactacacac ctactctgca gaggctcaat tatctgggac agataattac aaataataat
    39481 gaagcgagtc gttctcgagc tgtcttcaat gattctcctt agggcagtct gcgcttcaga
    39541 agtcatctca tctgcctcat caagtataat tattctaaac ggtttctcgt ccctgttacc
    39601 ggtaattagc ttcatgaccg cggcgaattc cttaaccctc tcccgaacca tcttgatccc
    39661 tctttcgtca gacgcattta actcgagagt atccctccgc cattcttctc ctaacagctg
    39721 tcgcgcgata cacaacgcgg ttgtagtctt tcccactcct ggaggacctg caaataacaa
    39781 atgtggcagc tcatttggaa tattgataag ttgatgcaga gcgttgacaa tttctttttg
    39841 gtctataact tcatttagtg ttaacgggcg atatctctca acccacatca aatttgagag
    39901 atttttgttt attgttggca tgcgtggtgg aatacctgac tctttttata tcataataag
    39961 ttaactaaat aactcgcctt cgagtttagc tcgcagctta acaaaagctg tcctgacaaa
    40021 agatataacg atcaataatt atatactcaa cacagttaga ttgcctagta atacttcttc
    40081 agataatgag gtcaaaatta ttcaaggcgc ttatcgcata gaatatttga tggaagaagt
    40141 tagggtatca tacaaacgag atgtaaatgt gtctgtaggc ggcctcacaa tcgacgcaaa
    40201 ggaaggtgat ctatcatccc ttcctcgatg ggtagcaaaa attttgttgg accaagacac
    40261 tgttgaaatt ccagataatc acgtctctac atatatttcc agagctttga atcgcgaaag
    40321 aatagcaaag cctcatgatc tgtccggaat agataccgat ttttatataa gagttaatga
    40381 ttatttagag ggtttgaagg aaagagaaag ggaaaatctg atagtttcaa tgaactcgtt
    40441 catagcttct cgtctcgaaa aaattgtcaa gctcgcagcc gcgtctcctc tttcaccaga
    40501 actaaaagaa aaactctcag ctgaagaaaa tgaactctat attcttgttc acaattcttc
    40561 ttcaacattc aaacagagag tgttaaagaa atttgtctga gcaacaaacc ttagcggcat
    40621 tatccaacaa tttagaaaag tttttacgat catttaaaga taaagatggg acctacaagt
    40681 attttgatag tattaaccat atgatggcct cgaactctgt ttcacttgta gttgattata
    40741 ttgatttcga tacatttagg ccagatcttg ccaagcatat cacacataat ccagacgaca
    40801 tgttagaggc atttaatgcc gcggtgttat caattctaac cgaaatccac ccagattacg
    40861 cattggaaat acgagataaa atcaaagtta gaataggaaa ttataccgta cagaagggct
    40921 tgcgagatat aaatgccgag gtaatagaca aattaattgg agtttcgggt atggtcgtga
    40981 ggtcatccga ggttaaacca cttgccaaga agatcggcta tcgctgcctc aactgtaaca
    41041 ccattaccga agcgcaattg aaaggactac aactgaggca acctttgaag tgcataaact
    41101 gttcagagaa agagcttgag atggatccgg aaaatagcat ttttagtgac tttcaaatgg
    41161 taagacttca ggaattacct gaggatctac cagctggtca gctcccacac tatgtggaag
    41221 tcacagtgat gggtgacctt gttgatcaat gtcgaccggg ggacagaatt atgttaaccg
    41281 gtattatcag aatagagcaa gaccaaatct ccccgcaagt aaagactagc ctattccgct
    41341 tacgaatgga aggaaataac attgaatatc ttggcggacg ggccggtagc aaggagatgc
    41401 gaactgtcga aagaatggca attagcacag aagatgaaaa acagatcttg gctatagcaa
    41461 gcaaaccgga tgcatatgac aaacttatag cttcttttgc tccccacgtc tacggtcacg
    41521 aagtaatcaa agaagcaatt ttattattaa tagtaggatc agtaacaaaa aaactagaag
    41581 acggatcaac tcgcagggga gatctcaatg tctttttggt cggcgatcct ggcactgcaa
    41641 aatcggaaat gttaaagttc gccgctaaaa ttgcaccgag gggtctttat acctctggac
    41701 gaggtacgac agcagcaggt ctgactgctg ccgtaattcg agacaagtct gggataatga
    41761 tgctcgaagc tggggctgtg gtgttaggag accagggttt ggtttgcatt gatgagtttg
    41821 ataagataaa gcctgaagac cgctcagcat tgcatgaagt tatggaacaa cagacctgct
    41881 ctgtagcaaa aggaggtatt gttgccacat taaatgcacg aacttccatc ttgtcggcag
    41941 cgaacccgct ctatgggaag tatgatccat tcaagaatat aacagagaat gttaatttgc
    42001 ccattccgct tttgactcga tttgacatca tttatgtggt tagagacacg ccagagaaag
    42061 agaaggatag ccgtgtagcg agtcacattt tggagcttca tcgtgattca ggacgtgcag
    42121 cgcatcctgc gatcgatata gatctgttca gtaaatatct tgcattttcg aaacagatcg
    42181 aaccacggct aacgattgaa gcaattgata ttattaaaaa ttattacatg aaaatgagaa
    42241 atgtcgactc cgaaggtatg atcaccgtta cacccagaca gcttgaaggt ctgattagac
    42301 tatctacggc aagggcgaga ttgttgctaa aagacaaagt ggataccgat gatgcggaac
    42361 gtgcaatcta ccttgtgcaa agaatgttag aaactgcggg agttgatgtt aacacgggaa
    42421 gggtagatct aggtgtactg catggcaagc ctcacagcga gatctccaag ttgaaggtat
    42481 ttatggaagt ttttaatgca ctttctggac aagataaaaa tgatgtagaa gaaaagaatt
    42541 tcattaatga gcttgcaaag acagggaaat tcactgagga ggaggcaaga acttttatca
    42601 aaaaggctat gcaaaacgga cagatatacg aaaggaaatc gggctcttac gccaaagcat
    42661 aaaaaaagta tttgcaaagt atgagtgaca taataaaatt tactcggtca gattgagact
    42721 atcttgaatg gtcctttcat tctcactcaa gctgacatat agatgataaa tatctccagt
    42781 attactacta gaataattcc aaataagtgg gcttagtggt aattgtctaa aagtagactt
    42841 ttgttggtct tccagtacct catacactaa ttggccgatt atgatttgat cgggctttgc
    42901 gagtgtagtc atcttggttg cgatgctcat agtataacct aatatatcga gctggggcct
    42961 tttcgataca atttttccat ctatcgtgcg agtctcccat ccgtattgta ctaccgcatt
    43021 ctcaccaaca tctattccga ttcttacatt cagctcagga taatcatatt gatttagtat
    43081 tggatttaga ccctcccgaa ccactttgat catagagcaa gcgcaattta ccgcgttaat
    43141 ggacggcaga tagaagtcac tagagtcaac cataaaaaaa gctaggattg catccccgat
    43201 gtattttagt acatatccgc cgtatgctgc tatcattaag gacatttcct gactaaaagc
    43261 ctggattatc gtcgcaagcc tatcgacagg aagcgtcatt agcagtctag ttgaatccac
    43321 caaatctaca tgcaaaatca ctagagtaac cttggacttc gcatagattt ccagaatgtt
    43381 ttgggtctct tccattgaga tgttaaagtc agatcctact ttcagagccc cccacattct
    43441 ggattgtgct agtttaatag ctttattgat gttaaccaca tcatcaagat aaaacttgct
    43501 taacagtgga acgtcagaga catttttggc ggttattttt cttctttcct cttcgctgtt
    43561 ctccctttga ataattcgca tgacctcctc ttgggttata tccaactcaa gagcaattat
    43621 atctggcatt attccggagt tatacaaatt aagtatagtc tgcctctgct ctgtagttat
    43681 attatttcac ctcgttatca taacaatcaa aattgcgaat tataagtata gctttgatta
    43741 ggaaaacata tgattttatc tatgttggct gtatatcatt atcgaaggca attacaaact
    43801 actctgcata tggatatgta ctgtacacca atttgttacc ccagatttac agcaacatat
    43861 tcgagtgtat tacgattgtt tagcaaatct tgactttgaa aattgcagct gaacaacagt
    43921 atatgaatta cttattaatg aaatttgcta ttaaacacga atgcctgcag aataacaata
    43981 tccaagaggc atttgctttg ccgctttctc gtctgtaggg acgttattgt agcttgataa
    44041 tctcatccca ggttccattc gctgaaagca tatagtattt gcttgtgttt ccagattcaa
    44101 attgtatgat atctccagct gatatttgga tttgaaataa tttgtttagg tactgaagaa
    44161 agctaggtcc tgccactatg cttttggctt ccatttcagg tggcttgttg tgatattttt
    44221 gcagcgcatc gactaatccc aatgtactaa tttcttccac caggtctgcc aagtccataa
    44281 acatcgaatt tcgcctgtag actctaagta ctgccaataa tagttacatt cattccatga
    44341 gtatacataa tgtgatcttg agaggtggtg ccaactaagt attgatgaaa aatccctgac
    44401 cgatctaact tggttttcaa tcctatactc agaacctggg ttctatcatt aatcagcagg
    44461 gctcgaatta aatgttctat aataattctc catactggat tagaagccaa cgatatggaa
    44521 taaataatgt tacttgagaa ttaatcttat ggaatgtgca gtatacgata catatgtaca
    44581 aaagagtgat ggcaaaacga tgcactttga cgttgtggta gaggcggata cttctcacga
    44641 gaaggcagtt gagtatggca agcggtatct cgcaagcgtt ggtcaggcgg agcaaaaaat
    44701 gactcaagaa gaatgtcaat tctgtcatat ccaggaagca cccccaccaa ttgaaagaga
    44761 tattgcttcg aacgggtatt acatacaaaa gatggaaggc tgtccacaat aaaaactgaa
    44821 tttttaaata aatttatttt tttaagttct aaacaagttg tatttggcaa tcagcacaca
    44881 ttcataaagt actttgttga taatggatta gtggtcgaag atcctattga caggaaaacg
    44941 aaggatggct agaaattgag aattggtcac agacagatgt aacctctctt ctctcttctg
    45001 tacgcctcat aaggaaccac attaacgtgc tagcagctaa agtgaggcaa tggtccctgt
    45061 ttcatttggt atcgaaagag gtgaaccgtt agcaagaaag gggatggagc tcctccccac
    45121 aagattaaga aaagggatcc aagtaccaat ctgcgacgaa gagctggttt tatcgtttcc
    45181 gtgaattgtc agaattcacc cggatgcaga tgcatacttg ttactcgact aaatatgcgt
    45241 ttcagccgtt attgataacg ttatataata tcatatttac gaaatatctg tgattaatag
    45301 agaccagaag aatctttgtc caagaatcat ggttgttgat gatgagaagg atattttacg
    45361 tattttaaag cgtgatttag aatctagtaa tttcgagaat agattcaatg ttgaggtatt
    45421 ttccactagc gagtcagctt tgaatgcttt tgcaagccat cctgatgact attatgatct
    45481 cgtactcaca gacatcagaa tgtcaaaaat gaccgggttt gaattatatc gtcgtttaaa
    45541 agaaagaagc ccatcaatca aaatcgtatt cctcacagca tttgaaatag atagaaaaga
    45601 attcgataag atcttggcgg atgcccattt tattaacaaa cccgtcagca tctctaattt
    45661 ggtccccaag ctaaattcaa ttctcgacaa aaagtagcag tagcatacac gtcgatcaac
    45721 ttttgacaga ttcgccacgg gcttgtgaga cggtctacac aaattcaggt gtaggtgtaa
    45781 acaaaggttg gatagctatt gggcctcaat agaatttgaa cctatttcga gctatcgtat
    45841 ttaatgacta aatacgttag ttcgtttact gccaaccaat gtacgttata ggtattattt
    45901 tcgaaaactc atttatccaa ttaaaaacca tcagccgatt tgcagcgctt gctcaaatca
    45961 gtagcgtttg cagcagtaca tgtatcatag tactggttac ttagcacggt ttgagagcat
    46021 gctggtaaca ttcacgggtt ggcggacttc agcttcttaa cgagttcaga gccataggtt
    46081 tttacttcat cattaatggt ttttcccata agctccatga ttacttcttc tcccttatta
    46141 ccatgtttcg ccaat
//
LOCUS       DAOR01000153            3464 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_65569, whole genome shotgun sequence.
ACCESSION   DAOR01000153 DAOR01000000
VERSION     DAOR01000153.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_65569"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1260 a          538 c          676 g          990 t
ORIGIN      
        1 tggatatgcc aggcctatat cttccacctc aattacaatc aaggttacta gaatacgtta
       61 ggtctgtagt aagagaagat gggaactttc agttcattat tgtaacaagt tcatctgctt
      121 tgatagataa agccactgct gaagaacttt tcatactgat gccatcgcag cagctcgcgg
      181 agggttctaa tatcaatagt cgtatatatt atattatagc tagctatgaa accgatatct
      241 atggctaaat ttagatttgt aggcagaatc agtaaaatgg gtaaaaataa tcgtgttatt
      301 tggattccaa gagaatatca tgataagata ttaggcttgg aagacaagga catacgtatc
      361 atgatagacg acgaaatatg ggatttaaag aaatgaggat aagaatagga aatgacaaac
      421 aaagcagcaa aaagaaatct aagaaggcag gtaaatccac caaatgacca tcatgttgaa
      481 taatatcaga gcggaacaaa gaaaaagacg aatgcagaag cgtgaattta tggtgattaa
      541 taagaaatga ccactactgc tattaacaca actctaagta tagctgactt tattaagcac
      601 tttccaaaag atagcatgcg tgacaaacaa caatacgtat taaatgaaat atgtaatgct
      661 tttaattctg cttataaata tattctactc gaagcaccga ctggctttgg taagagtcca
      721 gttgctgtag ctgtaggccg aacactaggt agtagctata tttgttctgc aactaaagac
      781 ttgcaagcac aatatgttaa tgattttcca tttttaaaat cagcagttgg catgggtaat
      841 tataaatgtc tagtgaaaga agattttatt agaaataaaa aatatgaatg tgagtcatgt
      901 tctaacgctg gtataagcaa tctagacgag tgtgaccata ttagtgtcga atacggcact
      961 tgcaaatcca acgagtcatt cagacacacg cctaaagact gcaatgcttg tacagatggc
     1021 gatggtagtg aacatatggg ttgtaaatac agaacatttc ccagagatta tgaaataacc
     1081 aatttacgtt caactagcga acagatatca attagtgatg aggctagaga acgatatgaa
     1141 tcaaactaca atgctgaaaa ttggatgtat cttagaaact tcgataaaca aagggatatc
     1201 tggcaagcat gttcatatta tgaccaacga aatatagcat tattagctag tcataccata
     1261 tttaattatt ctaattttct tagatttcta cctactaagc ttcttgaatg tagagatttg
     1321 ttagtattag atgaggggca tttaatagag agtcagctag tcgaatattt gagtctctct
     1381 atttctagaa aaaaatggag aaagtatatt ccatatttag aattaaatgc tgatggaaat
     1441 cttcatgata ttgagaaatg gcttgatttc ctttcaggtc ttcaattaca aatggaagta
     1501 aagattgatg aatggactaa gaataagaag ctcgttacag atgaatttct aatagaagca
     1561 acaaaagata aggaaaaact agatcaaaca atagatacca ttactcagga tagtgagaac
     1621 tggattgtat ctgaaataaa ggagtgggaa tattttaaca ctagaaagcg aagaaatgag
     1681 acagaaatag ttaaagtcac atttaaggcc cttgacatat ctagttactg caaaaagata
     1741 ttcgagaaat gtgataagac tttgataatg agtgctacta ttcttaacca acaagaattt
     1801 tgcaagtcag ttggacttgc agaagataaa gtgaaattta tacaggttgg atcagacttt
     1861 ccagtacaga atagaccaat ttatgctttg aacgtcgcga ggctaaacta tgctactcta
     1921 cagcaacaag aagttatcaa gagtattgcc aagtcagtag acaagataat gactctgcat
     1981 aagaacgaca agggaataat tcatacaact tcatattcat tattaaattc tattagagat
     2041 aatatctcgt tagtcaacaa acgaaggtta atagttacaa atccagagat agatagacat
     2101 gaagttgtaa agcagcacat agacaatgag aaagcaagtg ttttaatttc accgagtcta
     2161 tatttaggtc tcgatttgaa agatgacttt tctagatttc aaatcataac taaaatacct
     2221 taccctaacc taagtgatct atggattaaa gcaaagacag agaaaaatca aaagtggtat
     2281 gattggcaaa cagcactaag attgatacaa acatatggta ggtctataag gtcaaaggat
     2341 gattacgcga agacatatgt gttggatgcg aaacttggag atttcctgat gagaaataaa
     2401 atgccagatt ggtttacttc agcgataacg agattctcat aatgactatt aaaacaaaac
     2461 gtgtctatga taaacctgaa gaaagtgtat taataatgag aagacctata atgatgaatc
     2521 cagcattgaa ggaatttcga aaggttgcag gagtaagacc tgaaagacat atgattgaat
     2581 tatctcctag tgcttcattg gagatggaag tcttaccaga ataaaataat ttattgggat
     2641 acattcgcag aacgttatct tggagaacag aagaataatc cagctagcct agaagctata
     2701 catcagttaa gagagaaaaa taaggttact gacgttacgc tcttgtgcca agagtatgag
     2761 tgggaacatt gtcatagaca cttgctgaaa catttgatat actcaatcct atgttttaca
     2821 catagctcta acagatccag tgaaacgcat ttacgtagat tctacagaag gacacgaatc
     2881 aacatttacg aaaatgactc gtcttctatt cagctgctta gcgcgtacca tttattgtga
     2941 gcgtagcaag agaacttata aatgctggat agtgttcacc gtatacaaag ttgtctcctg
     3001 cagaatattt tataagaata gtgccattaa taagatattg ttcactttgc tcttctgaga
     3061 tgccataatt gcaatctaca gtaactttct gacctactat tgagtttatc ggaacatttc
     3121 cccagttata agcttgacaa agggtaggag caatgatttg ctgaaatgta attggactag
     3181 ggaaaaaaac aattgtcatt attccactat cctgctcatc tggtccaagt atatcactgg
     3241 gactcgcaaa ggtcatagac acggttggta caccctgtgg agtggtcatc tctattggac
     3301 gcgtgaatcc cgcaggaaag attattgaaa ttccatgcgc gctataaatc gtttgcccat
     3361 gagcagtgcc tataaataag taaaaagcag ttataataac gacagatatt ataaaagtcg
     3421 tcactattga atagattttt agtgctatgt tgcataactg agtt
//
LOCUS       DAOR01000154           27301 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21836, whole genome shotgun sequence.
ACCESSION   DAOR01000154 DAOR01000000
VERSION     DAOR01000154.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 27301)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27301)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27301
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21836"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    447..713
                     /estimated_length=267
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8929..9179
                     /estimated_length=251
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22535..22621
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8321 a         5089 c         5019 g         8267 t
ORIGIN      
        1 tgcgatggtg gttcaagctt ggtgatggga cctgcttcgt tgtaaaaatc tgcagtaata
       61 tatccagcct ttttacctcc tatttccata aaacaataac ctttgccatc aaaccgagga
      121 ttctgtgatt tattcttatt agtatttgtt atttcatcaa tgatttgttg tgatactacc
      181 ttggcttctg cttccgcaaa tattccagct ttaggaatgg caacgtattg attaacttta
      241 atttctgtaa catcgccaat ggcaaaaaca ttttcataat ttgttctaag agtaaacttg
      301 tccacattga tccaattttc tccttccttt aatagacccg gagaatttgc taccatcaca
      361 ggaattttgt gtggtggaat acctatcagc acatcataat ccatcctgtc tccatttctg
      421 aattctaact ccttctcatt taatacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttctaac
      721 tccttctcat ttaatactcg ttttagctta tgaagtggat ggaaattgat attgtgtcta
      781 ttgagcaagt taacaacatc ttgacttatc ttcggtccag ctacaggtaa tgcaatttgc
      841 gtaggtgtat atagatccat attaatagaa tctcttattc cactttgagt taagatatca
      901 tcaataatta acgatgcttc atatggtgcg ggggggcatt tatagggaat atctgtaata
      961 caaatcgcta tgttcccatt ttttattgaa agtatttgtt ctcttaattt tggaacttgt
     1021 gttgcatcgt ataaattaaa tcctccgttc tctgtaaagc catttatttg ttcaattgca
     1081 aagtccacac ccaatcctag tatcaaataa tcatattcaa tttgtttact taatttcgtt
     1141 ctaatgattt ttttatgaaa gtctatttgt atgacgtcat cttgaagaaa ttcaattccc
     1201 ttgtttttta gattatttaa agaaacctta gaatcttcta attttctgtt tccacctagt
     1261 atccataaat ttacaactcc catcaaaaag tagtcttttt tatcaataac tactatgtga
     1321 tgttctgctg aaagaccctt tctcaacata ttggcagccg ttaaccctcc gaatcccgca
     1381 cccaagatca cgatacgact acgtgtctct aacaactaac aaagtacaat accatctaac
     1441 cccatgtaaa aagattgtta ttaggaatat tatattatat aaattgcctt atcattcttg
     1501 atacccttct atatcatagg tcagtttcaa actttttaaa tttctcttgc ctcaacaaat
     1561 gtgatgtccc ctgtttcttc atctgtatat gctacaaaac gtctccaatt tccaacgatc
     1621 aagtttgcag acttcagcgc agtccatgat atgggattga ttcataattc cttacagaat
     1681 tctaatattc aacaataatt gcgtttaagg gataagaaat tgtatctcgt acattcaaag
     1741 aaagcaataa gaaaaacgga attattggag cctctccccc cctcggtgac gtattctacc
     1801 ctttattagt taagttgcga aaccttccta tactgttaca gcacatgcat gaatatctta
     1861 cattatttaa taagaatcga tcatatggcg aatgtatcga atgtaataaa agaacacctc
     1921 atatctgtct taaatgcaat tattgttact catgtcatcc gaagatggag agaattgaaa
     1981 aagaagagca agcgaagaaa ttaatcaaat atctatatag tcagaataat aataatcaaa
     2041 aaggagcacg acaacaacgg tatccaataa catttgttac ctatgcgata tcaagaacaa
     2101 gaacaactaa gtgatttcgc tatttgggta ggacgattcc atcttttcga atagctactg
     2161 tggtagaaga aaaggaatgg aaatcatttc ggaactcatt agacaaatct gatagaaaaa
     2221 tatttgatca aatgttttcg gtagctcata tgtataattc cgcgtcgtct tatactacca
     2281 aacctgtaag gatacagccg attttgatgt ccataatatt ccaccactat aaacaacttg
     2341 taaagctgtc taaagaaatt aaagaaggta aagggaaaag tagtagccta acatctgtca
     2401 gtgaaataag tctgccaaaa ggttgacgaa aggtgaacga aagagacatg ggggaattcc
     2461 cgctaggttg ttcaggctag aattatcctg atactgtgat agactggagt atcatattct
     2521 aacatggaca ccaaaagact tagatattat tctgaattct ttaatacagc ggaaatggat
     2581 tttacctttt atgagaaatt aaatttcaat atgaccaaag ggacattaaa tgaatcttaa
     2641 aataatcaag atacatctca tgattaagat tgaaccttaa acaatggtgt gagttagatg
     2701 agcgtatcta tattggagag acagatgagc aatataagca gtatgctggc tttgattaca
     2761 gtgaaaaatt aatagaacag ctagcagaaa tcaaattata taattcaaag atcttcattg
     2821 actttttttc tgatccaaga gctctacttc ttggcgcaat agaagataca gcttattcta
     2881 aaaacaagaa gcttgaacta gaattacata atacaagaaa tacgaaaatc gtctccacaa
     2941 aacacaaatt caaagattct tttgtcaact ggagtacttg gcggcaattc aatagccttg
     3001 aaaaaaatca attaaatagg aaagaagtgt ttgatgaatt tattgcaaaa actaaatata
     3061 tatcacctat tgttgaaagc aggttctcct caataaaacg agtttaccga gaacatcaaa
     3121 tcgttaaaga tgctaaggat agtgataaga tatctccact ttctagctat ctagaaaatg
     3181 aaaatgtatc atacaatcaa ctgattgagt ttataaaatc catcgggaat agggccaaga
     3241 aaccattcag agatgcattg acagatatta gtaaaaagat gcttggcaga gaaccagagt
     3301 attatgacga cttttatttc ttcagaaaca aagtgtattc tagcctagaa accaattttt
     3361 cgtcaataaa tcctcttatt gaagtgagaa agatactagc cttcatgcaa ttcgatttag
     3421 ctaaaatcgt atttgacact gaaagcagga aaaataaata tccatcacca atctgcttct
     3481 ttgtacagat accaaacgat attcgtatcc tctataagga ggaaagcccc tattttgatc
     3541 ttcaaggatg tttccatgaa tttggccatg ccatgcacgc aagttcgata gacgcaaaca
     3601 acgaatattg gaataaatac agaattccaa tgggcatagc ggagatattt tctatctttc
     3661 ttgaaagatt gacaaaaaat cccaactaca tacgatcatt gcttccgtca aataaaggaa
     3721 atattgatga tatcgtcagc gaaatagcat caagaaataa gtttatggaa ttattttttg
     3781 ttacatttta cacggcaaat tcattaatga aattagatta ctggaacaaa aacttatcag
     3841 tagatgaggc aagcaatgta tattctgcgc tgatcaaaga atatgttgga ttcgagatac
     3901 caggagaata ttggttattg catcacattc ttccggaagc aataatgtat gtgcccagct
     3961 acttactagc agcagtaagg gcagctgaac tagatattta tatgcaaaat agatttggag
     4021 ataaatggtg gacagaaaga gagtcaggca aaaatcttag cgaaataatg aagccaggcg
     4081 caaacataga tttatcaata ttctcaaaac ttgatagtac aacttatatg aaagaggtca
     4141 cggaaccagt gtaagtacaa aataacataa cgacctaata ttgtctgtta gaaagtacta
     4201 ttaccacggc tttagatgtc attttatggt acgttcaaac aagcaagtag cagaaatatt
     4261 agcagcaaaa acaataacaa ataaagaaaa aaagatttag aattagatag aaagattgct
     4321 ttaataattg aaggttttac tcctgccaag ttctgctaat taattttgcg agactgaaat
     4381 aggctatcta aagaaagtgg atagacaata tctgaatata tcatagcaat gaaaagagag
     4441 atcaatccaa gacttagcac cataaggact aattcaattt gtatctgaat tatccaaatc
     4501 tgttggactc gcaaagaagt tcaataatat gacacaggaa tatattcttc tctccccgga
     4561 tagttgtaga aaatcagaaa atgataggta tcactgaatt cttcctgaga tgtatcgctt
     4621 ggttatgttt acgaaatgtc tgttctttat tataatgcat aactgaatga cctgttgtgc
     4681 aatattcgac atggttgaat atggaaccta gaccgctagt tgttgatgtg ctatcccatg
     4741 tattgcttta atgctatgta taccaaacaa ctagtatggc atacttcact atcggcatat
     4801 tccagccaga cgcaaacatc tatgtcatcc tacttccttt ctcccattct ttttgttaaa
     4861 acctcttggt cgttttttct tttcgttttg ctgttgcaag tatttcagca gatccaattt
     4921 cagtcttctg tgggggaaat acaagatgtc tttatggatg gtctggtgct tactccaaca
     4981 tcatcatgcg cgaattatgg tatctttcga cagaagacca attcaagtcg aactatgcag
     5041 tcaaggctaa aacattcatc ctcaccgata tagctcattt atattaggac gagaatcact
     5101 tacattacag atgagttacg gtagaaatag cggttatggt ggcggtggta atagaagctt
     5161 tgacagaggg tctacagctg caaaaccagt cgaagaaggt aaggaatatg aagtagacgt
     5221 tacggaaatc agtagacaag gtgacgggat tgctagggtg gaaggatttg ttgtgtttgt
     5281 caagaatgca aaagttggac agaaggttaa ggtcagggtt actcaagtag gaagtagatt
     5341 tggtatagca acagttgttt aattactctc gttattgata tccacatacc tttttgtagt
     5401 cgcaagtact aactagcaat aaatctttta attctacctc tcttccacac gctcactcag
     5461 aattgtagat gctttataac ctttaaaagg cttgtaatcc tttcaaatgc atgcagaaag
     5521 tccaagcaaa cgtattccga aaacttgtga tggctatatc actagtgcta cttcttcttg
     5581 tgtccacagc gattattcag ccactgaatg cacagaactc tacggtatct ggtgctggag
     5641 ggacctcggg taaaacaact ataacacctg caggcaataa gacaattata atgatctctc
     5701 ctggccgcaa cacaactcct ggagcccagc ctgttgtcag caacagtagc agcgccacta
     5761 cctctggtgc taattacaat agaacagggt tcactaatct gtatgttctc acattcaacg
     5821 gaaaaacgtt cccaattaaa tatactatca caggcggcaa actagttgga atgttggcag
     5881 ataaggatag aagtacgttg gtactagtcc ttaatcctgg tccaaatggg gggaacttta
     5941 ctactgaatt acctagaaac ctgatagact ccaaaggagc ttcaaatgct gacacaaaat
     6001 atctaataaa gatagatggc aagggagtgg attacaagga ggtagcaaac aacttgaatg
     6061 caagaatatt atctattgac tttagcaaag acaatagatt tgtggaaata ataggaactc
     6121 aaatggcatc gtaatacaaa gtttcataat aatgaccagc aattattggc ggtcggcggc
     6181 gtaatagcac ggagacgcag atcaaaataa agaggtcttg gaacattttt ttgactttcg
     6241 gagatatggt ctttatataa tatgttctca actaagtcac taatgcacaa aaatccaaag
     6301 tttgcaatag tcactacggg attcatggca gccgtattga cacttagtat gttaacgaca
     6361 gcgataacat tttcttctcc actagcatca gctttgactc tgccaggtaa tcaaactagt
     6421 agtactggtg gaaaaaatat gacgatgccc gggggtaata tgacattcgg aacatctctt
     6481 cagaacgcta agatgcactt gatggaagct ataatggatc taaagactgg taatacgaaa
     6541 gcgacgatga tggagctaaa tttgacagct caagggataa agatgcatga acaagaaatt
     6601 aagagtatga tgatgggggt gaaaagcatg atgatgggga tgaaaggcaa tgcgacttct
     6661 ccttccacga tgtctaagaa tgcaacttca tgacggattc aaaataaacc taatgcggca
     6721 cagtttgtaa gactcctcaa aagtaacttg taaaatatcc cgagcattag gttcaaatga
     6781 agttattaca agatactgtt atagccaaaa gaattcttct catcatgcag tcatacctaa
     6841 taggtaaaat aatgaccgat aattttttac ggagttttca ttgatgtatc gtaaactgtt
     6901 tccctcatca taggactgtt ttttttcctc cttgaccttc cataaaatac catcagctct
     6961 gaaaacacaa aaccaagaat tctggcatag tgaacgatga ggttatattc ctaaagaagc
     7021 gaaagtaccc ttaagcatct cattcttaag atagcggtct taaccatcat aaaaatttaa
     7081 gtagtacttt tcttgagtat ggaataatct ggaaaaaacc ttaaatcatt cagatctaag
     7141 ttatatttgt gatcgaaaaa actgggagca tttctactac cacttcgttc gttggaggac
     7201 tcgctgcagg ggctgtggct attgcaatat cgctgattat acgatcattt gctgcaggac
     7261 ctttcatccc cgaacttgca tcccagactc ttttctcgct taccccagga caatttgaat
     7321 cacaagcagt agcgaatttt ggtcctctgg ctaaatactc tgcgtttgca ggtgccatta
     7381 ttgttaattt tatattgtat ggcctgtttg ctatatgggt tgacagatta cataaaaaga
     7441 tccattggaa gggatatgta ggaaaagcaa tactctcgtc ggtagtagct tacgtcatct
     7501 tactcattgt gaccatttcg cttgtaacaa taacggaaac tcgtactcac acacaaccaa
     7561 cttctatcat acgcttagtt ctttccctca ttctgcctca gatcgccttt ggctttacac
     7621 tatcttcttt ctatgaaaag gtacggcgac catcaagaaa acctgttgtc gaaaaaccat
     7681 cggaggctcc gacagagatc gattataaga aaagggctct acttcgcgca gctatagcat
     7741 cagcagttgc tttaccgatt ctatatttgg gattgaatcg tttgttttca acacaagaag
     7801 tacaactccc ttcatcttct tctctttcat tattaccgtt gcaatcaaaa tccaaacctt
     7861 tgggttttga agatcccaga ctagcacctc ttcttgattc ggaagtaaca ccaacatatc
     7921 tattctacag aatagacata aatcctatag tacctgtggt tgatgcaatt tcttggacac
     7981 ttactgtgaa agggctagtt aacaacccct tgacaatcac ttatgaagag ataaagtcta
     8041 tgccctcagt caaagagtat gccacgctac agtgcgtaag caacaaaata ggaggggatt
     8101 tgacaagtac agcattgtgg aagggcgtac ggttgaagga tgtgctccag aaggcacagg
     8161 tgaagccagg agcaaaatac atagcagtca gatgttacga tggttacgat gtcggaatac
     8221 ctttggaaag aggacttttg gacgggagca tattggccta tgagatgaat ttagctcctc
     8281 ttacttcgga acacggctat cctgttagag ctattgtccc tggtttgtac ggaatgatga
     8341 atccaaagtg gataaccgag atggagcttg ttgataatgt ttatgaaggt tattgggcta
     8401 ggaatggttg gactaatgta gctacggaag atactcattc gttcatagtt atcccaggac
     8461 aggcaccagt aagggttaga tttagaaatt tagacgtaga gaatataaat atcgtacctg
     8521 gtcaaaaggt gccggttgca gggatagctt ttgcaggaga tcgagggata gcaaaggttg
     8581 aggtgagcac agatggtggt gtcacttgga aatcagctag cataaaggat ccgctctcaa
     8641 aatatacgtg ggtcctgtgg actgcaggat tcactccagc cacagctcaa gggaattaca
     8701 gaattgtcgt tagagcaact gacaaaacag gaaaagtcca aacagctgag gtaagaccgc
     8761 cattcccaga cggagcaaca ggataccata tgatcaatat ataaaagtgc ggcaaactta
     8821 gtttcaacag tttacgggcg agtattacat tacagtacaa aattagaata ctagcatcga
     8881 tacatagtaa ctcacttcta cttttctggc gacaatatga atgaccatnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     9181 aaaatcatca gctaattgaa aaatgtcttg tcaagctatt ttatcacgac ttctccttgc
     9241 tctaaacggt attccaaata gattatctac tctctgtgtt taagcggtat tcgagctaca
     9301 ttgagaatat ccttaacctc accttctcag attaactctt attgaatgcc tagtgcctgt
     9361 ggactgatta tatcatttgc tgcctaaaac tcgttcgtta tcatcgtatt ccgagggcgt
     9421 ctctcagatc tatttcgtga tcctgctcct ctgctataat atccctcaat gcttcagaaa
     9481 gagcaaattc accagcgcgt tccgcctgac ggattctctc cctgtagctc ttgatagttt
     9541 cttgttcagc tctgaggtca atctcaagca tcttccttga ctcttgggaa tattctgctt
     9601 ctttgccttt cacggtcggg tctccgccca agtagtcgat ctgcctagcc acctccaggg
     9661 catgtgcgag ctcctgcgaa gcgtgctttt tcaactgttc agcgatgtct ccatactcgg
     9721 cacccttcaa tgtagaactg aagaccacat actggataat cgctttgtac tcccttgcca
     9781 aatcttcatt aagtcctctg acaatttcga ccttgctaga cgatgaggct ccttccgcgt
     9841 cagctgctgc agaattcgag ctatttgaca taggttcacc ttggttcact ctttaactta
     9901 agtgttttca aatcatgcta tatgcgtacg tgactaagag ttagtaaata tcaaacacat
     9961 aaagttaatt tgttttaata attgattaca ttttgcactt cttttgacag cccaagtttc
    10021 aacaacaatt tcaatatcgc ctatctattg aagagaatac tatcgagact tgctgttatc
    10081 ctgttctatt atgttaacca aatgagtcgt tgtaagctaa gtagcataga cctcaagatc
    10141 tcatacatag caagtacgta atatagatcg aattgtaata tgtataataa atgtacaagg
    10201 aaagccagta cacgggttac ctgaaggatc tcagggtgat ggtactcaga ataggtatca
    10261 ccgtgggatt agttacaacc ctttgtatga ctttcagtat tagtatcttc gattttaacg
    10321 gttacaaaat tccactacta tatccagggc ccttgaataa cctagcaact caaataatcc
    10381 acattatgaa ggagaatctg ctccccaaaa acgttaacct tatacaagtt acgccacaag
    10441 gatctttcct tactcaaatc tatgtcgcag ccttgatagg tataactttg gccgttccaa
    10501 ttacgattag ggagttagca gcatttgtgg gaccagcact taatcaacat gagaaagcaa
    10561 ctataaaaaa aattacaaca cctgccatcg gactttttgc aataggctgc ttattctcat
    10621 acttttttgt tataccaaac acactcaatt ttttgtacaa atatggagag tcaattggcg
    10681 ttagtacttt ctttaacgta tcagaattta ttccatttgt aatgcagttc ctaatcgcat
    10741 ttggattttc atatcaattc cctctaataa tgtgggctat tacgttattc aagatagttg
    10801 agcctacgtt ttggaggagc aatctgaggt atatgataat catgtttgtc atcttcggtg
    10861 cggttataac cccagatggt agtggaatca ctatgtggtt cgttgccgga ccaatgcttc
    10921 tcctttatgt gcttgggatg ctgtttatag agtggaagat taagagagaa tcctaaaatt
    10981 actaaggttg aaagtgcatt aagcactaag gcacacacca agtaaccttc ttaatcagct
    11041 cggggatttt tgaccatcaa tgatacttga tatgcgtatg ctgagtacca atgtcccaaa
    11101 tttcagagat aaaaaaatct taaatttaca gaagtatctt ctgagcctat gaaatttatt
    11161 gcatttcgaa gttctgctat atgaattgga ggaggttcta tatactcttt ctaacaaatt
    11221 cttcggtaca ggcaatccat agcggttaat agagtttttt tccaaactct ggagacttta
    11281 gactcttgcc attgattata tgtatttttt tcttatatga atcacgactt gcgtataccc
    11341 aaagagtgaa atctgcatgc cagatttaaa aaccttataa taaatacttc aggagaaaat
    11401 ccatgtagat ggaattttcc tcttttcatc cgtaaccaaa tttagcaggc gatactgctc
    11461 ttactgttgt tcttcccaat tgaactttct agcaacaaat gatgattgct gttataaacg
    11521 tatcaaatct atttttatta taaagacgat cattcggttc agatgcttta atttatcttg
    11581 tagctaaacc catttcgtaa ccacaagaag ttgcaaacat gcaggttttt gcgaattacc
    11641 ttcaggttca gtcatatcgt ttatttaatc atcatattag acctgtaaaa aatgcgacaa
    11701 caatgattgc tgtgggttga accccctatt ttgcttttag caacaacctg tgaatttttg
    11761 tcctaattta ttgccaatag tgtgaaaaat ctactctaga tgcatcatag agttatctct
    11821 ttttcctccc tgttcgctcc atttcttttg ctaaagcctc accggctttt tcatcaaggt
    11881 ttaaaaatct tgcaaattcc tcgggatctt ctttattatc aatcataatc tggatcagat
    11941 aaggtaagta tatcgagcca aaaatactct ttgatgtgtg agcttttctt gctagatcta
    12001 atattaaatt tttcattgat tggcctctcg caaagatagg cgccattact ggccagatca
    12061 tactatattg gttgtatttt attcccttgt tgcgagagtt ttcgaataaa ccatacgcga
    12121 ttatattgtc tagatacttg aggaggctcc attgcctatt ttgtccgatt cgtcctacta
    12181 ttatgtctac tttcgagagt acgtcaagaa gcgcagccat actattgtag ttaatacgcg
    12241 aggagactat actggaaaac agggcgttaa tcttatcctt tcgccttgcg tcagatgaaa
    12301 taccaaagtg tgggtctgga taagttgcat ccgcgtgtga cagaaacgct tttgcctcct
    12361 gcggagaatt agaagaaaaa taaccgttta ttgcacttgc aatatctatt tcaaagatat
    12421 cttcccgcat gacattatag ccggcaactt ttccttgcgc actatttaat aatgatcgga
    12481 tatctccacg gcagcgattt acaattaaaa ttttttcttc agtgtccaaa ttcttattgt
    12541 ggttttttag aacataatct aagaacatga tcaactctct aggaggaaca gggttaaatt
    12601 ttacgatctt gcaaactttt gcaagttgtt taatttttgt attatcttga gtattggcag
    12661 ccatgatgac agggatggtc ggttctttca tcattttaac aagagactct attccaccag
    12721 cgtcttctct tccagaaatt ccatcaactt catccaagaa taataacata gttcttccag
    12781 atacacttgt attgttaaac attggcatga tcagagtttc gagatcttct ctattcctgg
    12841 tatcgctggc gttcatttca attaaatcat agtcaaattt gcgtgccaat acttgcacga
    12901 gggttgtctt accaacccca ctaggtccaa ctaatagcaa aggcctactt ccatcaatcc
    12961 atcgtgataa ccatttcatt acagccaacc tggcatcttc atttcctatc atttgctcca
    13021 ccttttgtgg gcggtagata tcggtccaca tcatattgat tatataccga aataatctat
    13081 cattaaatat attctttgta gactgtaatc tggttcctgc aatgaatgtt ctccttcccg
    13141 accacgcaaa acaaaagtaa tgctagaaca tgagctaaac gaaatatacc gtttattact
    13201 agatggtaca agcgcacaag aaatcaaagg agccgaaaca taagcgatag aaattaaacc
    13261 aaatatatga aaaaattgag agaccacaag cgccgctttt aatattgttc tgtttaaatt
    13321 gtaatgcagg gtttagctgt cttggacttc atgttcaaag agtatgtcag actagtttgt
    13381 tattcagtca aatgattggt tgaatcttat atatgatatg ctcgttcgag gaccatattg
    13441 caaattgtga aagcacctac ctggttgaga atgcttgaga tgccgcgaga tacaaagcag
    13501 aagatgtatt gtgctttggt aagaatatct cccacaaaag gttacagaag ttactgctga
    13561 agaggttcgg aaaaaagatt caaggaggtt ttggaaaaca aggagcattg atgaatattg
    13621 catcggttga aaatgaaatg ttgatgaaac aaataaagtc aattgtatgt tagctcatta
    13681 gttgtttgtt ttattgatgt tggccgacat ctaagaaacc tcaaacctag gtactgatca
    13741 tagagaaaaa tactttaccg tttatagttc agcttttact ctttttacta aatcaaacac
    13801 atctattggc tttctaataa aatgtcctcc gctgttttcc aatatcgtgg ctagaacatt
    13861 ttcatagtct ttggttcgat atcgccccgc taatacttca taattgactc caaaagctat
    13921 tataaaagat acccgaattt tgctatctat ttttctgatt ttttcataaa atgtaaatcc
    13981 gtccattctc ggcatcgcga tatccagtaa caaaagatca cacaaatcgg ccttgaaatc
    14041 gtttaacgcc aagcttgggt cgttatatac atcaactcgt tctttgaatc cattctcctc
    14101 aagtgccagc ttgaaagtga aaccaatatc tggttcgtca tctacaatta gaattcttcg
    14161 cgtcatgtga gagcggtgct tgggaggctt ctcagattct tgtccttcag tcgtatcttt
    14221 gtcggccttg acttcttgtt tttgcaggag taatacagag aaccctcttg cgcttagaga
    14281 atctgagaca gagactattt ctacgtaacc cccatataca gtatctgtat gatgtgtgat
    14341 tataattcct tacgtaacac tattacgttg tgaacagatg cgttatcaat atcttttcta
    14401 acactgcatg ttgtcacaat ccttagtggc ggtagaacgg agtaggattc gcgatgccat
    14461 aataagctgt agcatacagt acaataatag caatagcata aaagttgatc aacagagtga
    14521 gtaatttaga acgtccttaa gaatcagtct gagacctaat tagattagaa tgtaatgaac
    14581 cactagctca ttgccgaagg aggcgacaca tgaaactgga ggtattaaag agattattgg
    14641 tttacctcag atcggctagc aagcatcacg tcacatgctt tgctagcctt cctttttctt
    14701 tagattgcca acggtatact ttcaaaatta attagtcctg taggaaacta ttagactgag
    14761 gaattcttgt acccttgttc tttatcatgt ctataaccag agcagcagtc catgagaatg
    14821 attttcaccc tagcccttca cctgtgaatg ggtcataata tactctaaat ccatgatttg
    14881 caaccagctc gagaattcct tgccttatct gttctgcagt ttctctgtat agtatatttc
    14941 aagaggtctg accatattat ccagttcgta ttgacccagc caataagtta agtggctaaa
    15001 atatggatct gtcaagtcgg tacatggcac agccggtgtg tggcatcgtc cttctccaca
    15061 cctgcgagca tgccttagcc acttcacgaa tagatcaatc tggtcttctg gtatcactcc
    15121 agtgtagata ggtataagtg atgatattgt tctctttctt atcctactta taagatcata
    15181 attacaaaat agtatttcct cagtttttgc cgaaactttg actggcatgc taacgataat
    15241 acctcgttag gtattacaca ttttctcttt tggttggtgg tcagctaagc tctacatgaa
    15301 tactgtaagt gaaatatttc attgtattca tatcgttaat gatgttagcc caggctaact
    15361 tttatatgct accgtagttt ttttatatac ataaaccaat aatgcttttc cagaagtatc
    15421 ttttagccag gaaacaatct cttcttaggc tatttcttta cactggccct gctcttatgg
    15481 taagtgtggc ctacatggat cctgggaatt atggtactga tattgcaggc ggagcgtctc
    15541 ttaactacag tctcctatgg gtagtttggc tttcgagcgg tatggctatg atgcttcaat
    15601 atttatctgg caagattgga atagcaacag gacagtcgtt accagaaata atccgtgaaa
    15661 aactaaaaaa gaaaatattc atcgtgccgt attggttagc agcagaagca gcgtcagctg
    15721 ctacggactt ggcagagtat cttggaactg ttattgcatt aaatctacta tttggtattc
    15781 caatgattta tgcagctgtc tttggagcat tggatgtaat attaatactt gcgttgacaa
    15841 ctcgcaaatt ccgtgtcctt gaacaagtgt ttatactgct catatcaata atcagtttcg
    15901 gatatttgta tgaagtattt atcacgaagc cagatccatc ggctatccta taccattcct
    15961 ttgttccact aattgcaaat tctaacgcat tactgataac cgtgggaatt attggagcca
    16021 cagtaatgcc acatgctctt tttgtacatt cttggtttac aaaaaataaa agtaaagaca
    16081 aatctattca agaaaagagg atattacgaa aactacatct tagagaaaat gtcgttatcc
    16141 ttgtaattgc aggaatggtc aacgcagcta taatgattat ggctgctgct gcatttaatg
    16201 cacactactc tagtatagct tcaatatctg atgcatacaa aaccttaatt ccattgtttg
    16261 gtgctggtgc tggaatagtc tttgttatta ctttgctttc atctggtatt tcctcttcaa
    16321 ttgtaggtac tctggctggt caagcggtga tggaaggctt gttaggtatg aaagttaaca
    16381 tctggctaag acgaattgtg acacgattta tcaatgtaat ccctaccaca atagcactat
    16441 tgctcggatt cgatcctctc actatattag tctacagcca agtcatactc agtttaatga
    16501 tacccttgcc aatgattcct ctggtattat tgacaagaaa caaagaagta atgggcgagt
    16561 ttgtaaacag aaatattaca acaataattt caattgtttt tgttggaata atattggcat
    16621 ttgattctta tttaattgca actacaatca agttataaaa atgatatctg aatcgttgca
    16681 ggatattcga gattctgagg gttataaaat gcgcattgta aaccgcttac cgaatttggg
    16741 ttccgactat ctagcaacta ggcagctgcc tggcttgtga gtacattcgc aaatgcattg
    16801 ctaggattca gctgaagtga caagctcgtc aaacttctat tatagatagt tataacaaca
    16861 aatacattag taacaaaaga gctttaggta gaaagacaaa cacagctgac tttgtctttt
    16921 ttcaagaaga attgttgttc taaattgctc taattgttat cgatgatttg atatgaggaa
    16981 attagatctt ttaggcaaaa ttctaggtca aggttgtcat gctgaaatat tggagagtaa
    17041 ttcgtctgga ttattgttag tggtgaaggc tgagaataga ttaactatct ctgttggcgc
    17101 catgatacga aatgaaggat tcagattaag atattgctac agcgaaacag atggtaaaac
    17161 tgtaattatt tttagcaaaa gcagcagtaa cgatggttat gaatgactat atgtcaaaaa
    17221 aaataatggt gggaatatgt gttaattttc ttcaattgcg tccttttaac ttagatcagc
    17281 ggtgtatgca ggcgctattg ctggcattat ttaagcaagt caagttacga caaacgttgc
    17341 tcggcttacg tcttgactca ctttgctgct caaacggtgt tcctggacag tacccatatt
    17401 tccgtacgtt tatagtcttt agaatgaaag ttcatttgta aatatatacc tcataaactc
    17461 taaggccagt tttctgactg attcagactc aggtcgtcgt atcctttctt gaactcgctg
    17521 acaatgttac ttactggctc cctcacctac ggaattatcg ggtacgatgt tggctcttgt
    17581 atcgcctaca atggattgtt tgactagctg cattcggatt tccgcgtcta ttactcgtag
    17641 catctattag taaataattc taggcattat ttttttatcg tcagatagat agtcaaatgc
    17701 atctgttact tcataaatat gccgattgag aggcagttcc attccatttg acgtcttatc
    17761 tgacgggctg tatcatagct tgaccgtgac gccgagtgtt agcattaaca cgaaatggtg
    17821 atcgtccata gtaaaaaaag caattagttc cagcaagact tttatgattg tcgcagttta
    17881 catggattcg accaacaaat gggctttaac tatcccaaac acaggatctc tttagcagtt
    17941 gtttgtatga ctttgttatt atttagtaca tcttcatggc cacagccaac gccggtctat
    18001 gccctacaac tttcagatca atcagctatt aaattattgg cttcatcatc aggttcttct
    18061 cctttcttcc tacctccatc atccagttct caaactaaaa gcaccaattc taccacagca
    18121 ggaagcgagg tcaagatcct gccttatgag aattctactt acgggataaa aatacaatat
    18181 ccttctaatt ggataaagca agaatcgcaa aaccgaagtt ctaatctcat cgtcggcttt
    18241 aaatcacccc caggaacggc aattgcctac cttactataa aaggtggaac acaattgcct
    18301 caaaatatga cacttgaaca gtataacaat ggatcaatta aagtattaag acagtctttt
    18361 cctggtctta atttgatcga gtcaaatgcg actactcttg caggcatccc tgctcataaa
    18421 atagtgtaca agagtctaag acagtcggga cgcgagcttg agttgatgca agtttggaca
    18481 ataaagggtt cgaaagattt catcattacc tatgttgcgt tatcaaagga cttccactct
    18541 tatttgccaa gtatgcagaa gatgattgat tcatttgcat tcgtaccagt aacaacacca
    18601 tcatcctctg cttctctaca agctaccaat gctacgactg caagagcgac aacaacagca
    18661 ctaagcaatc aaacttcacc accatcatca tttcttccct ttacgaatca catattcaac
    18721 ggttcatcac tctccggtat tgtgggcata agcagtgtaa acggtgtcaa agttactgga
    18781 gtaaatcttg gaaataatga aatatctata acattaagac atatactaac aactggaatt
    18841 aataacgtta gcagcgttcc accttcagtt acagttacag taataagatt acctatgagt
    18901 ctaaaagatc taatgtcatt agctgcagct tcgggcagca tgggaggcaa tagtactagc
    18961 atgatgatga ctaacaactc gaatcccttg aacactatga taggccaacg gtttagtgga
    19021 gtgggagcaa atgctacgac acagagcaat ccactcaaag cattagcatt cttgaaaaat
    19081 attcagatag gaagtagcag catactcaat gctgattgga gattacctca aactactatg
    19141 atggggttgc ttggtgccag caatggatct tcatctaatt caaccgcaga tttcgtaata
    19201 gtcacagtcg ttccatttac aggtaagact aacaatctga ccgggtaaaa tagactattg
    19261 tttacattca tgtgggccgg aattctatcc ttcacctttc aatatctatc aaccgaactt
    19321 caaattgaaa tatttgagag agcattttct gatagattaa gctatatgtt tgaattagta
    19381 gaggatgaag atctaagaga gaagtggata gaaaaaaaat ggacaagtca taaagtgcgc
    19441 ccaactctta tctatacggt tcctgttact aatgattgga tacattgcaa ttctatatag
    19501 gctgtccggg ctagagctat acaagaacag gttatctggt taggtgcata agatcagaat
    19561 tcatcctcgt tggatcttcc actaaaatca aaaattctgg aacatttaaa agagtcttcg
    19621 acaatgaatg gcggaagagc ataatagtga caggcaggca tgtctttgtt ttactcattc
    19681 tgctaatggg catttgtatc gttaatacta tgtttacctt cactcagact tatgctgatt
    19741 cacttagaaa cgctttgcct cagaggatcg gcaactataa tgttgaaatg aaaacagacc
    19801 cgacgactcc tatttcgggt cagaatgcca agattttttt gaaaattagt agtgttaata
    19861 gggatgatct tgtagatctt ccaattatta taaatatatc taaagatgga gttaggcaag
    19921 agacaacaca tccgatattt gtcccatacg gtcataacac agatgaattt atttttaaag
    19981 aacctggtat ctacgcacta gatgttacta ttctagatga cgtttataca ggtcaaaatc
    20041 ttactgtcac atttcctata actgtgatga ctccattgct tggttacttt tcactttctt
    20101 cctcttcgtc agtacctttt atagcaacta tcgttattat cgcaaccaca agtggagtaa
    20161 ttttaattta taagaggaag agacgagcgg caaaaagtat tgagcatacg aggacaccaa
    20221 atgaagatag gtattaatta ttatcccgga ttttcgctcc ttctgcacag tacggcagga
    20281 atgtgatcta taagacaatg atatgatact gaaaagattt ttggtatttc gttaactcga
    20341 tctattttac ctcacatgtg ctgactttca tctcacccaa tcaagcaccg atcctagttc
    20401 atgcgtaaat taagtagttg ctgtcatcat aatgtggaac ggtggtgtcg ggatatgggc
    20461 actaattggg tatgattgca attgtttcta ccgttgacgg cttcgattgc agctgtaata
    20521 tgataccaat acgctcattc caagtacatt cgcaccgaca ttgtaacaca ataagctggt
    20581 tttccggttt cttgtatacg cagaactcct aaacgcccta aattttaggg taagttaaac
    20641 ttaattttgt catagataag acaattcaag ttaatggaat caattcatta aatttctcac
    20701 aatacgggca ttgtaatacc aatcctaatc cgtctggggt atgaaattga tgctgtatct
    20761 gctgttcact cacattttta gcaagtataa aattacagtt accacaaaga aaacagacat
    20821 caccgtcccc catgaaatat gtgcctttgc ctgatgtttg cttgaggacc actctagtac
    20881 cttcttgtgg ctctgatgtt atcatacact ttatgtcaac cattttctac gttgacaata
    20941 agctgatata aacacgttct ttattgttct accacttcgg ccgcgtatta ttcccatgca
    21001 acctttcaca tgtgaggatt cagaacgccg ccgtgtctta cattcgtggc tgtattgtca
    21061 tccatttaaa acactatcta aatactgatg tagcgaaaat ctgaactctg cactctttac
    21121 cccacagtga atcaaaacct tcttcctcag cttatcttga tagcatcatt acatttcgtt
    21181 agttgcctat tcgtctattt gaggaaagcg atgccgagat tatgactgta ctattacatt
    21241 ttttatctga tttgtcccgt atccttcgct tgggtgtaaa gataaaagtc acacacttca
    21301 caaaaatagt agagactgtc gaagctagaa tataacgtca tggtatcgga gcaacgggga
    21361 cattcaattt caaattgtag ttctatcttt gtgctgctgt cctgtttctc attcttctca
    21421 tcttgttgat gatactgtaa actatttgaa gcagtgatcc gcgttactag ccttttgctt
    21481 accatattat tatttgactt cttagtcagg tatccttcac cgtaagttac caactgtgga
    21541 aacaaaagtg tattgccaat gaacacatta cctcaatata gtataaggtg atctatgtac
    21601 cattgtacga aaatggtatg ttgattgttg tatcgctgga gttatccgta acgtgcggcc
    21661 cggatcaaag tcaagaacat acgtgataac gccacctgtc gaatagtaaa atcactcaaa
    21721 tgattgctat aaatcctatc ctcataaaaa caaaatcagt atactgaaca aggcatatgc
    21781 tagtacagtc ttttaatctc gtttttccat gtacaaggtc aaccaagcga aggaatagga
    21841 atacatttcc aaaatacaaa gattttttta caatcttcaa atcgaagaag atacacacag
    21901 cgtcaatgga atcagatcaa attacatgag aattcagcct tcttaaaggc aaagatctga
    21961 gtgtttcaga gtctctaaat cccacaagtt ggtatgacgg atccccacaa ttatatggat
    22021 agctggcacc atcgacatca ccatgtacca cactaggaaa cctaatttac aagtactatg
    22081 atagtgaatg atccaaaacg ttcaggtagg tgatgaatat tgggagcaaa ttttattgac
    22141 acactccgcg tgaatatcgt tggtgctgaa atgtctatta tcaaacctaa tatccagaaa
    22201 acgaaaggaa aaggtggagg gacgaaagca gatacaaacc ataagcctgc tgcaatcgaa
    22261 aagccctata gaatatcgct ttacgagtgg gcatgttgca ggtacgtgtc ttccagtgcg
    22321 caagtactcg ataacaagta tggcacgtat tgccgcgtaa gaaagggcaa atccggagga
    22381 attcttaccc aagccgtcag gaactcttac ggccttgaaa gcaactgcag ccatctgcaa
    22441 aagtgttaag agacgatgtc ttaaatcatc agtttcaaat cttgaattat agaaagtaac
    22501 tcccatccac gaccaccaaa cctgtctctt atacnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 ngccaccaaa caggtatgaa tagtgcaata aatccaagaa acccggagat cgaaacatca
    22681 tgattgagat ttgtactcag ctgaaaaact acaatcacaa cgatcaaatc atagaaattc
    22741 tctaaccatg atgcatgtcg tgctttctca atattctcca ttcaagatta aagacgattg
    22801 tggatacctt ttatcctttt aggcgcatac tcgcaggtca tagaaaatgg cgaaaatatc
    22861 gagaacatag ccaagcgaga attactagag caaattggtt accttgctaa caaattcagt
    22921 atgtttatag ttatcctccg tcagtttgca tatcaataca gcttattcat gtctatcgaa
    22981 taaaggaaaa aaggaagttt gcaattgctt aaagggacta ttgcagtagt atacttgtta
    23041 agccctaaat tccattatat tcattttatg atggtcgtca tatgaaaatg gattattaag
    23101 tccttacgag cacacaccag cgaagcgatc ggtatcccat tactggcagc gctcctccac
    23161 ccgtcccata aaggagatat tgtgagatta gaatctggca gctatcatgg cagtacatag
    23221 acataacaaa aacagttcta tgctgataaa gtaagtttag aatggtctta cgcaactcct
    23281 cgtattgaac tataatcaat gcaagtgcaa gttaatggct taaaagtggt gttgagagat
    23341 tcaaaccatt caagattgaa agccagaagg tcgaaactca taaatcataa aaatgcattc
    23401 ttgaattttg atgtccacct atgttgctgt ctgggctggt cgagaaatgc catctcctca
    23461 tgttagagac ctttcaaatc atattgcatg tataaaattt agaacaacag ttgagaaagg
    23521 agaatcctaa ttgagtcaca agatcaaagc tatcaatgga gtcaatatta atgttgaaga
    23581 aattgggact ggaaagccaa ttgttttcat ccatggttgg ccggtcaatc ataacatgtt
    23641 cgagtaccaa tttacagagt tacctaagca tggctatcgg tgcgttggta tagatttacg
    23701 aggttttggg gactcggata aaccctggga gggttatact tataatacta tggccgatga
    23761 tgtcaaggcc gtgcttgata ctctcaacct ccagaatgtg actctggtag gcttctcgat
    23821 gggtgggcca atctctattc gctatatgtc tcgtcatcat ggggcacatg ttaaaaagct
    23881 cgctcttcta gcggcagccg caccatgctt tactaagcgc gaagattttc cctatggtat
    23941 tgataagacc gcagtagatg atcttatccg tcaaacctat gaagatcggc cttctatgct
    24001 cagaaacttc agccagatct tctttgctgc tcctgataag cttagcccag aattcaagat
    24061 ctggaatcta agcctgggat tggcagcatc cgcctatgcg acaatccaat gtgcaattga
    24121 actccgagat gcagatctgc gtaaagattt gtctaccatt caagtgccta cactgattct
    24181 tcatggagtt aatgatcgtg tatgtttatt tgatttggcc aaggttatgc atgagggtat
    24241 caaaggctca caacttatac aagttgagaa agctggtcat ggcttttact acgaagagcg
    24301 tgaaagagtc aactcggagc ttgttagctt tattggctag tccttttttt ctgtgattga
    24361 aagacttgtg tatcggttct tattatgatg tagctagcta gatgatattt tggcagtacg
    24421 ttctggaatc gttttggtgg ttgatagtac agtgtctatt tcaacactcg aaaagttccg
    24481 cctctgagaa aatgtttctg acatcgctgg aaatatgcgt tttttgtaga aggataaaag
    24541 catatgaaat taaaaagcat gacgtaacag tagtaggaga tttttaaatc acctataatg
    24601 ctagaagaaa agcagcttaa gcaagcagtg atgtcttttc tacaccaagc ctttatcgaa
    24661 gatggtgtgt atttaaagac aatgacgtag cctatctcaa tttttcattt atggttactt
    24721 attgtcgtca cttgtcaaag acccatcggt atttactagt gtatcacatt ttggaattaa
    24781 agcattcgaa ccacattttt cgtgttacat ccttatacaa cacataaccg cctggaacta
    24841 atcaaaacag ccacagtgcg tgagcgctta caccgaggat tattagggtt catagaatga
    24901 aaaatgacca cgccccaaaa tatgattcct tgaacatatg tgttacatgg ctgattaaat
    24961 cccagtagat aatcgtattg gttacagtag tcctctattg tcttctattg ttgctctgaa
    25021 tgctgttggg tatgctactg aatcaactaa acaattctgt tccgttaaga gttctagtca
    25081 ctgtatttgt aagagaaatg agagcagatt agcagaatct gcataacttt tttaataagt
    25141 ttgtgccttc atgcacatgt tagaaccaaa aactgttgcg aatactacgg ctaatgtcac
    25201 aagtaaacca tttgcatcgt atacatcaga agagatgagc cttggtattg taggattggg
    25261 caagatgggt gctaatctag cccttcaagc tgtggggaaa aatattgcag ttataggaaa
    25321 ggccaggagt acaaagccgg atctagaaag aaatggtgta aaagtagttc gtgattatga
    25381 atcttttgtc tcatatttgg acattcctag agttatttat ctatctttac cagctcgacc
    25441 cactattgat accgtgctaa aagaaatgct gctttatctt gagagaggag atgtagtgat
    25501 ggatgggggc aactctttct atatggattc tgttaaacgg gaaaaagaac tctttgacaa
    25561 gggaatatat tttcttgatt gcggaacaag cggtggactc gaaggtgcca gatatggtgc
    25621 ttgctttatg gttggtggca gagctgaagg tatcaagatc gcagagcata tcttaaaaac
    25681 cttggcggtc aatgaagatg ggtatgtttg cactggtcgg cctggtagtg gacattttgt
    25741 aaagcttgta cataacggga tagaatttgg aatgttgcag tctataggcg aaggtgtatc
    25801 cctactactc caaggaaatg gatttaattt agaccttgtc caaattttca aaaactggtc
    25861 aaatggatct gtcattaggg gatggcttgt tgagctaatg gagagggatt aagagaacaa
    25921 gattacggca aaataccaag ctacattgaa gataccggag aggttaactg gttagtggag
    25981 gacgcgatta ataaagaaat cccgattcct gtgatttctc aatcagtaat ggagcttttc
    26041 aagtctagga gcaaagatag tgatgcctat aaatcaattg cactaatgcg acatggtttt
    26101 ggaggacatc cgtttggcag agatgagcat ataaaaaagg aaagaaagac aagtagaata
    26161 actagaacat aaatattgac aattgaattg catttctcat gatgtaatca acctatcacc
    26221 agatgcgaag tgtcaggtag aggtaatcag taaatgtgta tagactacta ttcgaaaaat
    26281 atagcaggga tccaggaatt cccgctagtt atcgtggata ctccttattc tcttttcata
    26341 ttttagaaat caatcctatt ttacgatgga atattctatg caatagatcc tcattatagc
    26401 gatcttactt ttgtagtctg ttctagaaat aacatcaact acattcttca tggattcgaa
    26461 agctatcttg aatagctttg acgcgaccct aattaagtac aataaccttc actatgctga
    26521 tattatcgat atttttatag cgaactatgg agtagtatga aattgtaata gcgtagaggt
    26581 aaattgatgt gacatataaa gagccagatt ttgtccgctt ttctttagat tggataataa
    26641 ctacgctatc acgatctttc atcgcaaaag atattccgaa gaaggaggaa gaataggcca
    26701 acgtcaggat catcacagag ttctaattac ttttcttcat tcttttccaa aaatgaaact
    26761 ctaataaaat atacacttgt accatccatt cctctttcga taatagcaat tctatctatt
    26821 aatgctaata cttgggtact atctgaaata catcattttt atattgagat gtttgctgtg
    26881 atactcgcta caatactctc attctactac atatcgcgtt atcgtacttt aaatgataaa
    26941 ttcagtctct ttcttggaat aggatttctc gctaatgctt caattgattt gctgcatgtt
    27001 atagtatctt ataatttaat aaatgagcca ctctttctca aatattttat accacagaca
    27061 tggtttgcag gaaggctatt tctcagtgct atgtttgcta tggcaattgc gggttatcct
    27121 atattgtcta ggaaagaaga tcctaacact agtgtattat ctattgcatc ttcttttcgt
    27181 tcaagaaaga aaagacaagt acagcatcta tcgccttcta tgtctgaaca agacagaaaa
    27241 gaagaagagg agcaagaaag aagaaaaaga gaaagccgac ataacagatt acaacaaaaa
    27301 a
//
LOCUS       DAOR01000155            2577 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_124285, whole genome shotgun sequence.
ACCESSION   DAOR01000155 DAOR01000000
VERSION     DAOR01000155.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_124285"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          717 a          512 c          490 g          858 t
ORIGIN      
        1 gaataatctc tgcatgccgc tctaataatt ctccaactta taaacgcgtc taaattttcg
       61 gtcataatca taatcatttt ggacacaaat ccgtatgacc atgagtacaa caataacaag
      121 gacagtcgta agagactgca acagaacctt tagggacttc ttcgcactta caaccatgac
      181 agaacttagt catttggaca ccaactcgga tctgctttct tgattgcgtc tttgagatta
      241 tctattccgt tttttattgc ttcacgacca caattattat taaaaggctc taccattgtt
      301 gtacgatgtt tgatatcctc ccaaactttc ttgtctattt ctactgcctg aatatctttg
      361 aagtcattgt ctgtcttttg tgatttcatc aagtctatag attgaccatc attgatacat
      421 tcttgccatt cttcaagctt acttactctt tttgctagta tactatggca tgtctcttga
      481 tttctccatt cttcaagctt ttttaatcta tattcggatt gattatgtct gttgtcatta
      541 tcaggacagt aatgattttc tttccagttt tcaagatgac ttactctatt gattagtttg
      601 cttttccaat caaattctgc tgttccttgt tgaaggtcaa ttactttagc ttcaagatcc
      661 atgatacgtt tatgatcatg cattttttct gctagtccat cttgaagatt agtgttaaag
      721 acagcgatac cacaattgta actgatgtta acccagctaa aattgacttt tgcacatttt
      781 taggaatatc cacttatact tatctatatt ggccttctta aatggcgaac agcaattgat
      841 caaccaatct accaataatt tcatacttcg caaaaaccaa cacggaaaaa aatgctaatg
      901 ctccaccagc tctgcatttg tcaagatctc aacgtagttg tcctagccat catcctattg
      961 atagttattc gattgttgat ttacatatac cagatttaat accatattta caaaatatat
     1021 agaaaaaaat tgtatcatgc tattatatta ccagtactca tttaacaata acgtgctact
     1081 aaatgcagaa actatcgaga ccacttggag ttagtatcat tgcaaatttt ggtataatat
     1141 tggggattat agggctcctt ggcgtcgtta ttgtgtcagt tggcggtgtt aatatggcta
     1201 atacggtagc aagtagtcgt gttaatacag cagttggtag tggctcagta gggttagaag
     1261 ttgctctcta catccttgtc gtcggctatg gtttgaggaa aggaaaagca tgggcatgga
     1321 ctatgtctat aatagggatt ttgataggat ttgccactac cagttactct atctacttca
     1381 ctacactacc agtcataggc gtgataggta atcaagttgg acttacggca tatgccggta
     1441 taatagcaga tgtgatcatc ataagtataa ttgtatttgt tagcatcgaa ctaataatct
     1501 tgtattattt atacagacct catgttaaag catattttgg taagacaact ccaagctcat
     1561 cttgagctac tacttttcaa taatcaagca gtaccctctg tagcggaata gcatggttgc
     1621 aacaaaatgt agtgtccttc tgactagcca caggctgtat tttggtctgt tagattatgc
     1681 tatcgatatc tccgctgtgc atcggtggtt atcttgtcat ggctgcagga ttattctggc
     1741 ataagcattt cttcattttt aacgctaaaa agtggtaaga aagtggcaag agcaaaatag
     1801 gtagatggag gatctaattg aatactcgtc agagcagcaa ataatttttg ctatgttaca
     1861 gacagacaga cttagttcgt aacgtagtga ttgacaatac ttccaatcgc gttagccaaa
     1921 gtctggacga gaacttgtcg cctttctatg acgctgcgta tggatggagc catctctatt
     1981 tgtatcgcat ctatgccgcc tgcgctggag cttccgtagg tttgcactgt atatccacca
     2041 tccagatgct gctctttttc tcccgggagc ttcggtgaga ctacaaaccc tgcagatgtc
     2101 aagaaatggt ttaggctgaa atttccccac atggcatttg ggtcaacctg cagtagatgt
     2161 ttaatcgtag atccactatt cgttcccaaa tacacatctg cagggtcatc ggggaagacc
     2221 acgctcgcat ggatatcaaa cagaaatcct gctccattat ttccagctct aatttcgtct
     2281 acgaatttcc gtatggtatt atggtactcg ttatagtaaa tccctgcagt aggactctcg
     2341 tatgcgcatg ctggttcacg attggcatcg atatacttac gagcaaattc tgctatgaca
     2401 acataaggtg ctttaccaag attgttgaaa atggagtgtg ctacctcagc tgtgattata
     2461 cgggtatacg aatccggcag tgttctgaaa ttattgcaat gagcagagtc cgtacgttga
     2521 ttaactccag gaggttgttg attgccgtca tgtggacagg taagtattac tggaagt
//
LOCUS       DAOR01000156            9260 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_18671, whole genome shotgun sequence.
ACCESSION   DAOR01000156 DAOR01000000
VERSION     DAOR01000156.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_18671"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    6643..6885
                     /estimated_length=243
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2928 a         1641 c         1751 g         2697 t
ORIGIN      
        1 atttgcacca acacctcttt tatcgtaccg aagtactcca aagcctcttt ctaatagata
       61 ttgagctatc taccaaaacg gtgtaggtgg tttaggacca ttcttgtgaa caaatcccaa
      121 tgtttcattc ttgtcagtag gaccagatcc atggataagt aagactccag gaaatggacc
      181 tttacctaca gcaggaagag ttaattgagc attagtcttt aatccattcc ctaagtctat
      241 tactagattt ctgtatttta tagtctgtat aaacggttga gctagtgcgc tctgttgttc
      301 tttggaaata gtagcagcta aacaagataa tccaagtata cctataagta gtaatcccag
      361 agacagcgtc atacttttcg ttctatacaa cttgcaatac taccaatctg atacctgtat
      421 ttgaggtttt atggttaaac ctcacacaaa ggttagttgt tatggcttct ttctaaataa
      481 tgataatgat agtattatga aaagcaacag tattccctct ataagttttg aaaagagagg
      541 aaaaatgagc ataagtttct atggtatgtg cctcatgaat cagaagatag atttcataag
      601 taaaacttat acaaaaacca tacccaagaa tttcttaaat agttcccagc ttatcacagg
      661 atatgtcaaa tcacgattct attaggaaac tatgggttgt tatgccgctc tttattgctt
      721 ttgcatttct atttattatt ccgagtactg cagtattgat tagtaataat agtgattttg
      781 gtactaccaa ttttgtgtat agtcaaccag atcatatgaa ctccaatgtt acaaattcac
      841 taaacataca aaatatacca gtaaaaaaag ttcacgtagg agatattgat attgcttaca
      901 agacctttgg taaaggtgac cccattttac ttgttagtgg ctcttctagt gatatgaatg
      961 cttgggagcc atcgactttg agaaacctct catcgaatca tacagttatt atatttgata
     1021 accgtggagt cgggaataca acagctggta ccaaatcatt ttcaattcag caacttgcaa
     1081 atgacactgc tggtttacta gacgctctga aaatacaaaa agcagatgtt cttggatatt
     1141 ctctgggttc gtttgtagct caacagctta tcgttacgca tccagaaaag gttaacaggc
     1201 ttatacttct tggtgcatcg tgtggtggaa aagagggtgt tcctgaaagc cctcaacttg
     1261 taaaattttt ttctgagatg gtaaataaat ctataaataa tattcccatt actccccagg
     1321 aagtaaaaat gcttctatct atcccgatgg gatcagcatg gatggaattg caccccaatt
     1381 ttcttgaagc cattccagaa gccaaagatt tgtttgctgg tattccttct aataccataa
     1441 agcagcagaa tgacatttca caaaactgga tggcaaccaa ctggagtggc atttgtgatg
     1501 agcttacaaa aatatccaat cctactttga tcataactgg aactcatgat aataatgtac
     1561 caacagccaa ttctttaatt atagcgggaa aaatccccgg agcttggctt gtacagataa
     1621 aagatgctgg ccatgcatta ttcgtgcaat atccagataa agtcaataga gtattacaaa
     1681 catttctttc gaccacaaga aaccctggtt gacgaatata atatcggagg aggttagatt
     1741 atactcactt agattagact agactaacta tttttttatt tattttgtct ttatgaatag
     1801 taatattaga catagcgcac gataacagaa ggaactttaa cttactagac aggctaaatg
     1861 ttgaacccgt tattgcaata agaaataacg catctacaag agcaagagga tgtcagtcag
     1921 ttaagaaggg aagaagtact actaatcaag aaacttgtat atcaaggatg gaatcagctc
     1981 aaagatgcag gaaaaagatg gattactgaa ataatatttt cttcaatcaa acgtgttttg
     2041 ggagaagact tgttatcaaa aacattttgt gcacagaaag tagaagcagg actaaaagtg
     2101 atgctataca acaaattcat cagcttgtaa ctaacatcta aacatagtgc agaaccaaat
     2161 gtcaagactt ctggtatgga attaagctac agtctacaat agatgatatc tgtgtgttac
     2221 ctcagtatgt aattaaatag gactcataga gaactataaa aaggcaacta ctttccatca
     2281 attcaaatca attatgttag cattcatgct atttgcagaa tctggaagtt tatgtggctt
     2341 gatgatctct actaagtgga gaatgtgcat ggttaatatc tttgtagcta gttggttcga
     2401 caagggtacg tagaatgacg gatcttgttc acagatacaa aaatattgca aagtgtttag
     2461 agactgctag ctacagctat attttgagat ttcatctcat ttcatcttga ttttgagatt
     2521 ttcgttatag aaacaggcac atccttagat gatgacaagc ttttgcttca ccatcatcaa
     2581 catcagtaga tctcaatcag tagaccgaaa ataagttttg atgacctatc acaaaattca
     2641 tttcaatact cagtgtgctg atgctcgtcc attataataa tccctcaatg aatctttaaa
     2701 atatagatct ggttttcaaa tcgcagcatc gcaaccgttg ccatagtcgt caattgtccg
     2761 attgttgact gaaatcctat tcgcgaggat gacgaccatt ttttcaatgg atggacggct
     2821 atgtaaactt aaacttatct attgccttaa aagtgtcgag gtcatacgac aagcgtaccg
     2881 agatgtctgc tgaccctaaa gtacccgatc ttacacctat agttgacata ctaaaagagt
     2941 ttcggatcct ggaaaccata aaatcgaagt tgtttgcaag acctgacgaa gcatccaaac
     3001 atctgagtgc tgtgttgaag aagatcagtg gaagatacat aaccttggct gaaaatgtaa
     3061 ggatatttac cacattgacg tttgatagcg aaccacaaac aaaggaatcc aaagaatttc
     3121 tttttgaagc aaaatttggt tatctaacag atgagattgg agacgctaga gtcagctgca
     3181 gccgtatttt gaatatctat aatacatatt taagtggttg gtttttccgc tttcttaaat
     3241 cggatgaagc aaatcgattg gaaacccctt tttagagtga aagtttctcc ttacgataaa
     3301 gagtttgttc tagctgcgga tagaacaaat gaatttctaa ctaactctgg tgaattaatc
     3361 tatccgcttg tcgtagatgg tgatttgcaa caagctcaac agaaggttaa agaattctca
     3421 aacgatcttg atttaccgct gacagatttg agaaatgaat tgaaaatatt tctgagtatt
     3481 gaagccgatt ttctagaaaa aaggaaagct gtttgaataa aatattgaat tcaatcctgt
     3541 aaagttctgt tgcggaaacg ttctgttgat tgaggagaat gagtacgcta gacgttaatg
     3601 gaaccgttgt tgcgaaagcg cggtgggtga aagagttaat gagacttttg tgaaagatgc
     3661 ccctcaaaag ttgcttaacg cccaaccgta aaaacaaagc ctgttgtagt acaatttata
     3721 aaatgtaact gccaaaacat aatgactcat atatgtcaag ttacttattc tcaacaacat
     3781 tctaatgtat aagatattct taattaaaaa agagactata gaacattgtc taaatcgcaa
     3841 gaggaaatgc atgtgtggag aaagaaaata gaaatgatag gcattgatat aaaacatcta
     3901 gaccaagata tggacaccaa tcgattcagg ctaaaaacaa ggatcatgaa tcggcacttc
     3961 ataaatgaac taaataagat cggtttacaa ctcataaata tgactggaac ctttgatggt
     4021 aaacttatgg tacttttgga aacaaaagtt ttatgacggc acgaattgca gcgacgatac
     4081 cggaatctgc ttttgtatat ttggtatctt tcaatagaaa ctccgataag ttaccggaaa
     4141 agagcatgtg actctggtaa ttcttaggga cgtttggaag gtgtggtaat ttagggacat
     4201 tttctggtaa ctatttggga gcagctttta ctatataatg agagactatc ggttggggac
     4261 catctttgag aagtgcacgg attccatctt ccataatctg aacgtttttg gtaacgcgtt
     4321 catgctgacg catatggtca gtccaggatt cagcaattct gatcttgaga tagtgaccaa
     4381 tatctgctgg gtcttggaat aattcccaat aggccatccc ctcgctcttg agagtacgtc
     4441 ctaactcacg tatgctctgc tcgaactcat cagataattt agggtcaatc ctgtagtcga
     4501 ttgttatcag agcttggcct tcactgtttg gagggtcaac tgagagctgc ggaggaagtg
     4561 accagtggtc actgcctgct ggtgtgaaat cgagatcacc cacaagagta gtgctgtaac
     4621 gctttctagc aataataatt gcagctaagg ctatcgatgc tactgacagg gtaattggaa
     4681 tcccaattgt gttagctatt gtaccccata tcacacttcc aactgccaag ccaccattta
     4741 gtacaagcag ataaactgct gatactctgg ctccaatcca ctttggcgca gatttcattc
     4801 caatggtata gagttttgat aatatggtga tgtaagctac tcctcctgct cccatgacta
     4861 tacaaagaat accaaaatct cgtagatatc ccattgtgaa tattaccgct gcaagtaaac
     4921 caatagaacc tgtcataaga gattctattg agaccctaga ttgcaattat ggcaaaattg
     4981 caattccacc aattattgct cccgtaccaa aagatcctaa tagcagccca aaacctgtgg
     5041 aatcaagtct tagttcgtgt ttcgctagaa gtggtaagag agataataat gcactactac
     5101 ataatgtgaa catacccgca cgaaccatta atacatgtgc tgcttgagaa tggcgtacat
     5161 aacgtatctg agctcgaatg gctccaatta tattctccgg tggcagcgaa gtttggactt
     5221 gagactttct tggcaattta ctcaggacta tgatcataac aataaaggag gctggattaa
     5281 gaaaaaatac ggcccaaggt gcaattgcag ctacaataaa accaccaacc gtagggccaa
     5341 ccgcacgacc tgattttctc cctataacag tcaattctat ttcactcgat ctatcgagtg
     5401 cagcgtaatg tttggattct gacatagatt cgtttttata aatacagatt aatgcttttt
     5461 taatgtttaa taatcaattt tggatgaatc caatttatcc tgcaaaatat tagcatatcg
     5521 ttacaaacaa tttttctgga atgtaaatgg tctattatgc tttagaaaag tacctacaat
     5581 aaagttaaca tcttctatat actttgtcac cctaacagtt cttattccaa actgcaaggg
     5641 cagtggctgg ctttagttat ggattcagaa ccattcacag aaagagtaca tacaataccc
     5701 gtaataccaa cagccgaaga ggaagacctg atcacaatta aatagtttta agtatacgtt
     5761 atttatgcgg tgttggatag ttctactata aatcttcctt tacatggagg acatgcgccc
     5821 tcatacttaa tcagacgaat ggttaggctt tcctatgcaa tatccaaagt aattgtagct
     5881 gaatatggtc agcaggagtt tttaagaagg ctatccgacc ccctatggtt tcaagctttt
     5941 ggttgtgttc tagggtttga ttggcattcc tcaggagtca caactgtagt aacgggtgtt
     6001 ctaaagcaag cactaaatga agatgttcac agcatatcca tagcaggggg aaaaggaaag
     6061 aaggctagag aaacaaaaaa cgatattccc aagcttgctg aaaagcatta caacctgtct
     6121 tcttccaaaa tcgataactt attgtatgca agcagaatgg cagcgaaaat agataacgcc
     6181 gcattacaag atggctactc gctgtatcac caggtaattc tttttgatga gcacggaaat
     6241 tggactgtcg tgcagcaggg aatgaatcct aataataaga tggcaaggag atatcattgg
     6301 gtttcagatt atctaaagag ctttgtatcc gaaccgcatg caggaattat aagcaaatgc
     6361 aaaagtccag atactctcaa tatgacgtca attgattctg cagaaaatca aaaaatctgt
     6421 gtagagttga caagaggcga tattaacaac ctaaaatcat ctgtatataa aatagcagca
     6481 acgtatactc tggataagag gaatacgttg gacaattgga ttaccgcaga tacttcaggc
     6541 aaatatgaag tatctactaa taccacgaaa gatccctcga ctgaacatta ttggatacct
     6601 cgcagattag actgggatgt atttaggaac atttacgata ttnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatagg cgaaataatt
     6901 tttggagcca aagcatcgtg gcaagatcca gtgaaatata attttgccca tggtggtaag
     6961 gatggagttc cctatccaat tgcacgtaag atctatgata aatccataag ttatctaacc
     7021 tctgcaattg aaggagcaga aatagaaagg gaggagagaa tccaagcttt aaagaaattg
     7081 ggagatcttt cagctagaat gtttaatcga gatagtgcaa aggatacaat atgattaaac
     7141 atgtccagtg ttgagaagat acataataga gctacgagcc gacataggga ttaaaacaga
     7201 taatccgtgc actacaatgt gcttgaacat ccaatttcaa ttcgttaatc atgttatgct
     7261 ggagatatgt tgattggggc aagtttctgt agttgtttaa accacaaaaa agactgtctt
     7321 aaaaccttca atcattatta cgattatagg gctttcgttt gcagtaatgt ggaacaaccg
     7381 agatcccttc aactggtgca atagcaggaa aaaaaataag ctatgtggat acctcagaac
     7441 aggatgcgcg tcgatttatg aaagatttga ggcttaaacc aatttcatat agataaatcg
     7501 catcgagaag taaaattggt catgggtttc cttaattatg cgttccataa caaactatta
     7561 taatataaga ataataggtc atggaaagta ttactactac aagtgttgac ccaatcctta
     7621 tgcatgtttg ccaaccacta ccatcatcta ataccaccaa aggtgtgtga accaaatttg
     7681 acattatttt tggactcaat tgtgggttag cttatgaatt taggattcct taggacctat
     7741 aaccttcttt caaacaaagg ttgataaaga tgtctaagga cgaaattgaa atcgaaccag
     7801 cagagtttga atcagcgaat aaagaggcga atgtatcaca acgagtgaag acaacaatac
     7861 gatatctgga tgcagttact tcccttgaag gtttattgaa ataagcgggg accttcaagt
     7921 taaaggagac acagacgtag attacctagc aatgatgcga gaatccaagg cctttccaca
     7981 cgtgagatga ataaaatata cgaataagag agaaaatgtt atttttccta ttatcacaca
     8041 atgcagcttt catttcagat caggatcctt tagagtttca gtttcaagtg cgcttactat
     8101 agcgttaggg tggtcgggac aatgccattc cggtattttg tgcttaccaa ctcccgtgct
     8161 cgtcccgtct ttgtagattc gctggcggta aattctcgga caacggaata gccgggcaca
     8221 ctcgttgcat tctaggtcta caatagagtt ttgcacaagg tactctttac ctctaaaact
     8281 caatattttc tgcttgactt taattgtttt ttctttgatt tccggtggtt gaaccccgtg
     8341 agtaggcaga tatggagata cggattgtag caggtcaagg aggtactctt caaatgtaaa
     8401 tgattcttca gtagcagctt gagcaaaagt atagttcttg acgagtgttg catgagtttc
     8461 gtcgtctagg taaatttgta tgagattcat ttcttggctc aaattacgaa cttcgtgcag
     8521 atcagggttc catgctctaa ttatatgcca gtttcccttt gaaggaattt ctgaaacatt
     8581 tcaggaggac tagaattctg gcgaccgctg atatggtaga agcttgcact tctaatatca
     8641 tatctatttg cataattcag tttaccgctg gccctctagc acagacgtaa acttgtttgt
     8701 agcagagatg aagtatttgc gcgacaatgg ctttaaagta ataccaatgt ctgatttagg
     8761 atatgatgaa agcacaaact ccatgtatat aaaacagtag attcgttgac gagatgatta
     8821 atattccatt ctgatttatt tcaaattaaa tttattttgt ccagcaacat ctgttcagtc
     8881 cggtatgaca tcctttgaat cccaatctac tagattttca aataagaaca aatctagatg
     8941 gaggaattat acaagtcagt gatatgattg tcgcttccca atctaatgga ataaatagaa
     9001 ttgctgcgag ttcaatggat cctataccta atcctaatgc aatggtttga tacaggtcct
     9061 aattcaatgc ttaggtgact tttattactg actccatacc tgcacccata tgttctgaaa
     9121 tatggcagtg gaatgtccat aatcctggat tgtctgccac aagcaaaata tctacagtac
     9181 ttcctgtagg aacaagtacg gtatccttcc aacctaggtt atcatttacc cttccatctg
     9241 taattcctaa atatttacat
//
LOCUS       DAOR01000157            4755 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_86303, whole genome shotgun sequence.
ACCESSION   DAOR01000157 DAOR01000000
VERSION     DAOR01000157.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_86303"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3460..3605
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3923..4157
                     /estimated_length=235
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1299 a          813 c          873 g         1389 t
ORIGIN      
        1 gagtattcag atatgagcta gtaaatattg cggggctagg ggcggggagt tgtaatggga
       61 ttaactcatg tagtgcaatt cagaaggcag aagggagctt tgatttcgca aacttccatt
      121 gggcatgcga cgctacaatc tgtggggtgc cgccagtaca aataggtcga cttcccgccg
      181 gtctacttcc ccactagttc taggttaaac ccatcaaacc atccatccgg tgaaagatga
      241 cacaaacaac agcaagattt taacttacaa tattcttctg gtgccacgcg ctagttggga
      301 tcaaacaatc tcacctgtag gactctcctg aagtgaggaa ggcttataga gtactcatct
      361 ttattcttgt agattgttta aagttcaaca ctacatcctg atgtttggcg taagcattag
      421 cgccttggcc ttaataacaa tagtcggagt tatacaagtc ccttcagctt ttgcggctgt
      481 accgctcggc tgtactggaa atccgcatga taaggactct tctactggaa atcctcatga
      541 cccttcaggc acgacatcaa gcgggtttga gggaggaaat ccacatgatg ttggtggaca
      601 tcatgatcag catgagttcg acgcatgtcc tggttccaaa taggtaatgg agtaaagact
      661 aatgctcaat taactatcct cgctgttggc aagggtccgt tccctgctgt gcttcttata
      721 ccgggttctc gagcgattga catgaattat actgttaaaa atatcaaact gttttggaca
      781 gaagaaaact atctatccga aagaggcttt gcagtactca gatatgataa gagaggcata
      841 ggtgcaaatt ttgctatttt ggacaaaaat gtatggggaa atacgacagt aaatgatcta
      901 acacatgatg cagagagagc tttgaatgtt cttacgcagc aacatgaagt ggatccgaag
      961 aggataagta taatcggaca tagtgaaggt acagttatta ctccaagagt tgcaattgat
     1021 aattcgacga aagtaaagag tattatactt atgggcaccg tagctcagaa cttcagagat
     1081 tcaagttacc atcaatttgt gtacgtaccc tcagagtacg ctacacaagt attggataaa
     1141 aatcacactg gattgatatc aatatagcaa aaagcaaagg atcctttgtt agctattatc
     1201 taaattcttg gtgccgcctt ctctttttat tactcataat accaagggca taattatcac
     1261 tctaacaaag gaatttggta aacgacccca aggatttatt catttcatta ttcattcatt
     1321 agcctttgta atagcagtct taggtgttga ttttgtttta gtttttcttt gtctcttcta
     1381 gcagtggctg tagttctcaa tgccataccg caacaaggac agaacacacc gttgtggtaa
     1441 aagtaaacct cacatcttct acaatatttc ttaccacctt cataacggct cttaccaaaa
     1501 ataattttgg aatacaacct ctcacataga ttcctgcaaa ccatatcgaa ttgtaaggaa
     1561 aaggttggac cgctaaaaat atgggagcta cttacagaga gtgggagggt ttccacgtat
     1621 tattacgtaa acatcaggac tcaaaaaaga gatgtcgttg ttttattttg ttttctcaca
     1681 atactttacc aattcattca agaaatgaga ccaatattca tcatattgtt tggccttttc
     1741 atctgcttta aatccttgat gcatcaattt gacactcgtc ttgttattct cgattttttc
     1801 cagttcccat gttacaactg ttctcggaaa atcgggctcg tacgaatctt cccacgtata
     1861 ggaaattttc ttatttggaa taaattcgat aataatccct tcgtgagtgt tgccttgtcg
     1921 gtattcatta ttttcgtctt tacgaaaact gaacttcatt cttcctccta ccttaggttc
     1981 caaaatagct tgatctggga accaatttgt caattcatct ggatcagtaa tagctttgaa
     2041 aacaacttct ggcgaagcgt taatgactat gcttttgttt atttctattg tttcttgatc
     2101 aatacgatat gcaagctatg aatacggtta tgccaggcat ggccccagta catacacacg
     2161 atattagcgg tattattcat gtagagtcca ataccgttag accctatata cttggtgagt
     2221 ttttcaatat atggggtggt cttaatacta ccggcaaaaa agtaaaagct acgatggatg
     2281 gtgcaccggt atcagattat aagaacataa ttttgagatg gagaacaaat taatttatat
     2341 gttgaaaggt agaagaattg aaatattctc ttttaattac tcttctactc tttcatattt
     2401 atgcaaggag ggagagcgtt ttatcgacct gattgaaaca cgatggataa ctgacctagt
     2461 ctacgtgttc tggctctaag gttaaaagta atgggtcggg atacggccac gttagaagtt
     2521 tggtataggc acattggttc taatgaaatg gtaaatggat tggaatctgt atcattggaa
     2581 tccgtatgcg gggaatagcg attatgggta aaggatgatt gtgattaata ttattatcga
     2641 gattgccgct ggctgacttt gactgaacat cgttattgtt gttaatactg ttcgagcggt
     2701 agttgttagc attgaccgaa gtgggtacag tggacactct agtcacatta agactattat
     2761 gtgctactag ggtaggatta ttagcacaag agaacttggc agttattttg ttctgacctt
     2821 ctttaatgtt agtatactga ggagtaagag tgaatttcca ttttgaatag tcatccttcc
     2881 agtgagtacc gatgggtgta gtatgctgat atggttttat tccatttacg ataaccgata
     2941 cttcacaatc agaagtagtt gcattatcac cagatgttcc caatattgct acgtctttat
     3001 caacctgaat gtattgacct ttatgaggtg aagtaatttt tactaaactt aaattcggtt
     3061 tagttggttg ttggtgtaat atggatggag gactattagt agtatgatcc tgaatacccg
     3121 tgaatagttg tgcatacgct aaatgattca tcaacaaagt aaaagctaaa aagattaaag
     3181 ttagcggaag ctgtatggaa tgcaatttct tggagaagaa tatgtagcaa ttggattctt
     3241 gatctcgcgg ttgggtctca atcttctata atctcgatct acctccatag cagccgccaa
     3301 tatcttgctc ggtcttcgat ttgaagattc caaaaacatt tttgtatcct tcggaaggca
     3361 atgtccgcct attccttccc ttggttctaa tatctcaaca ttccatttgg tatttagagc
     3421 atctcgtaat tctggaaagt ttatgttatt tgcttgacan nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnngttta tgttatttgc ttgacagtag aggtaaaggt cttctgcaaa tgcaatttgc
     3661 aaatatctat gtgcattctc tataatcttt gataattcag ccatctctat ttttggaact
     3721 ggacgcattg gaattcctag gctttttctt ttttgttgct gatgatgttg ctgtttttgt
     3781 attgaggaaa aggaagaaga tgatactatc tcattattat tatttccatt tatcattgct
     3841 ccgtttaccc ctcctaccgc agcaacagag gaagaagaag atacaccatt ataaaattgc
     3901 aaagctgctt caagacaaca atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnaca aactccacca accactcgta gctgatttac actatgttct
     4201 ttttcttcga atgtcccccc cctcttcaaa aatatccttg gtagaggaac taaaagccta
     4261 tcacaaaaat aataataatg aggcgctatc tttgaccact tattccaata gtgaatcgac
     4321 cgtattaact tacatttcaa tatttgaaac actctgggcc cagagcgata aacaaaattg
     4381 aggattttcc gccaaagcat atatcgctaa agttaaatac ttctgattcc ttcgtattct
     4441 gaaggtttag accttctggt gggaaattga aatagaggta tctgtttcaa ccgaccaaga
     4501 ggatagtagt tctgttgata acaaaataac cccaagagtg tgagggttgg gttcaagtcc
     4561 cactccggcg ccatacttat ggggtaataa caagttaaga aaaaaattcc tttcagagat
     4621 tagaggaaca agtggatttt cgcattacaa ctaccaaaga atatggtcag aaggcaatgg
     4681 cgatattcgt acaacttatg agacaatcat attgaaagaa acaagctgcc aacgctatcg
     4741 ctattcctgt tattt
//
LOCUS       DAOR01000158            5413 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_82722, whole genome shotgun sequence.
ACCESSION   DAOR01000158 DAOR01000000
VERSION     DAOR01000158.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_82722"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    434..570
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4425..4650
                     /estimated_length=226
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4897..5163
                     /estimated_length=267
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1455 a          946 c          867 g         1515 t
ORIGIN      
        1 ttaatcaacc aaaaaccctt gattccaaac tcatagctga tttagcggtt agaatgttag
       61 tgccttctcc cgaagacttg tggattatcg tatacacctc gaacttcggt attgaggtac
      121 ccgcgtattt tacttagcta ctcggcaagt actttgacta atatttgaga atagacgctg
      181 ggaaatttgt aagttctaga agttctcaca tatctgcacc gaggaagttc caaggttcaa
      241 agcatttttc cgttatcact taatgagata atatgatagg tcacacacga tatagccgct
      301 tcgtgacttt tgctattcat tatcaattcg ccctttgaag tctctcatga catgcggacg
      361 ccggttttcg tatatcacaa tcgagttatc tgcaggatca cgaaatgtta agcaagggcc
      421 aactgggatc tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aattcacctt ctagcacctt tgtatagtat
      601 ctgatggatt cctggatatt tggagctgga acataaagaa aatcaagtga ttcgaacatt
      661 gtggtcatga cgaattaatt gtcatacacc cacacacaaa caaaattcat acatttcatg
      721 gtctgtgatt atctataagt gaaaaagtat ttccgtatgt gaaaatccta gcggcttcag
      781 agataaagtc atgaggaaat cccagctgga tcttgctttt ctcatctaat cttctcaatt
      841 ggtctgcggt tagttcaaaa tccaagcatg ccgagttgtc ttttatttga gcttcagttc
      901 tagctcctat gataggtatt atgatgcctc tgtcgtgttg ttgtcgtatc caatttaagg
      961 ctatttgtga aggagtccta ttcatttcat ttgctattgt ttgaacttct gttgcaatag
     1021 aaatagttct ctcatttaga tatgaagcac tcattggatt attgacattg aatctttttt
     1081 gctccttgct atcctgcttg atatactttc ctgaaagaac cccgccaccc aacggagacc
     1141 atgctgttac tccaatatcc aaagcacggg ccatcggcaa taattctctt tcagcagatc
     1201 tttcaatcaa gctgtacatt atctgaattc ctatgaaaga tgaccaacca cgcagatctg
     1261 caatagcatt tgcatgtgaa acaacccacg ccgggacatc agatatgcct atataaagaa
     1321 cttttccaga ccttatcaaa tcatctaacg atctcacaag ttcttcaatc ggagtcagag
     1381 gatcccatat atgcacccaa aggagatcta tgtagcgagt gtttaaccgt ttaagacttg
     1441 cttcgagaga ttgaaccaga ttcttacgat ggtttccact tgcattagga tcatttggat
     1501 ttgttgtgag ggtgtatttt gtggctagaa caaacttctc tcgttcagca gagacgaatt
     1561 caccaacata tttctcacta gtgccatttt ggtagacatt tgcagtatca ataaaatttc
     1621 caccctcact cacaaataag tcaaatattt tcttggcttc ttccttagaa gctccgggta
     1681 acatcgagcc ccagtcttct ccaaatgtca tagcccctag acatagctca gaaacccgaa
     1741 gcccactttt tccaagtagt ttgtatctca taaacaataa tcataagacg ttgtgtcctt
     1801 tataagaaat aaggcttaaa ttatacaaat tgtttgttca tcattacaaa tacctcttac
     1861 aaatggcctt cttttctttc tttgattacg aacttacaga ctaggtcgcc ttttgcagct
     1921 ctatgagtgg tttctatatc agctccaaga agtttttcaa acaattctgt ttcagtggaa
     1981 catgcttccc aatggttttc agctatgtgt ataattggac agttgtattc taagagaaca
     2041 tgttttccgt aattcttcgg ttccctcttc gattcagcca tatatccttc ttcatctcta
     2101 attttggcta attctttgac cattttatca tattccaagt tcctgagacg tttataatat
     2161 tgatcgaaaa gttcactctg gcgttcacgt agaaccttct caacagctgc cttgcccata
     2221 tttctttcaa tgaaatcaag agcatgtgtt gcaatggcac tatatgattt tgggaaaatg
     2281 gttttgcttt ggctcgttag ttgatacgtc attcttggcc tgccgacatg cccggtggtt
     2341 tctaaactct ctaccaaacc cctctcttgt aaaagtgcaa gatgtttatg aaccgccatt
     2401 ctagatattt tcatagcttt agctagttct tccaacccag cgtgttgcat aaccttaaga
     2461 taatatagca gcctcttctt cggttcgcta aatccattag caatattttc taaatttttc
     2521 gaaatagcca attgtacaac aaatagacta actcattcat ataccatttg atttacaaac
     2581 cataaaacta aataaattaa accacgttga atttattaaa tgcagactgc attcagttca
     2641 ctcgtaaacg attactctta ttatctcccg ctttcaacct attcatgtaa ttatctcaca
     2701 cgcaaggctc ttttagatcc tgtccccatc tttttgtgca atgattttat aggaaatata
     2761 ttgctattac ggcatggtga tcggaatcca tccacaaata tggtttctta ggtaagaagg
     2821 catctggtgg attcacaaac ccaaagagac gtttttgcta cctttcggcg atatttaggg
     2881 cagcattcaa attcttcctg tggatcgtag ccacgacgtc attagagcta gatctggcac
     2941 attcattaga gaaggaagtt agagagatgc tatgtaatta ctatagatgt atgtgcctta
     3001 agatgctaat agttgatgaa atgcaagaac atatgaggaa ggtccctaat accagaatta
     3061 ttaggttgaa gctatgatta atgctaacaa catagaatat ccctgcggtt gtagattgag
     3121 ttatgtcgga tcgattcagg gagatgtgct aattaataga gtaacctctt tgaagctttg
     3181 tgacaaacat aagaaagtaa ggaagcaaga agcctaaatg tcctaaatgc gaacaaggta
     3241 gtttcggctt tacttgaatt ggccaacact aacatctaca ttaatcataa ctatgtgtta
     3301 gtaaattatc tagatatcac tagatgagaa ggatctcgat agtatgatta gaaatagaca
     3361 taccagaaag agaggtgcaa aaagtgaatc tactacactt aaaaccacga attggtatag
     3421 gtatatttgc aatgtatctg agatatgagt tactgctata tttgtaagaa cgaaatcaag
     3481 aattgaagca accaacgtaa caataaatag taacagcatt tttttcctag tatccttgaa
     3541 tacgctatca cgagctaaat tccttaagga ttctaccagc agatatccat tttgctcttt
     3601 tgccaccaca tattggacca tccaggcact aaacaagaaa gaagtcagtg tagagagcaa
     3661 gatgatgaca acaccagttg gaataatatt tatgatgtcg tagtaagttg caatcttaaa
     3721 agtgtatagc gaaaatggat ctgattttag actgtatgtc tcacgtagtt ggctctcata
     3781 accgccaata aaaaaaagta ccaaggaata gatcgaataa attgtgaaga gctctaaaac
     3841 tcctatacca tgatagttgt tcttaggtgc ccctaggctg atgcatctct ttgataaatc
     3901 taattgaaaa taaataatca tgaaggtagc gaaaaatatg ccaaaaagta gtacatcggg
     3961 taaaaagact ttgttgccgt tgctgtagta gagttctctg gtgaaaacaa cctcgactac
     4021 aatcgatatt actgaaccaa ctaaaggtaa caagaaaagg atgggatttt tagaaagcac
     4081 aaatagacct tgtttaaaaa atgtgatatg agaagtcata tggtaattat ctttcgttta
     4141 ggagtttaat aattatctgg gtcttccttg atagatgaga gttatttaat tgatttcaaa
     4201 caatattact tttcgtaatc tgatgtcttc ggaaacggcc gtaaccagta ttgttcctac
     4261 tcagataccc tctgaagtaa actcgcgagt tcgtttggta gtgtaaccat tgcatcatgc
     4321 cctgtcttaa ggtaataata atcccaggca tatgactttg ctccccaggc cataaaatga
     4381 aaatccttga aatctgtcat gcaatataag ttttaggtaa attcnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtaaattcc ttccttctat attttgctac
     4681 aacggttggt catgtgtgtg ccaaggcgta ggacatagac gagtatccat ccaagcaata
     4741 tcttgttggt tggttaatcc ccgagctgta gcatcatagg gtgggacaag ccgagccttt
     4801 ccttgttctt tcatggtcat tgatatctct tagaccgggt accaaatcga aggcactcct
     4861 gcaatcctcc ggtatatatg cgtcaagata aactagnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnncgcgtcc gataaccaga cctccatagc tatggctacc ccttaattgc ggattctgta
     5221 ctatccgcag gtttcgttgt aattctgttt tgaattcgac gcgtcattga ttgaaatttt
     5281 ttggattact acttaatcag acaaacacat gaacaatgct tgagggattt cctagacaaa
     5341 ataataagga ttatgacata gacaaaggag gacaattaaa taaatacata tgaaatgtaa
     5401 tccatcgata ata
//
LOCUS       DAOR01000159           10444 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_1438, whole genome shotgun sequence.
ACCESSION   DAOR01000159 DAOR01000000
VERSION     DAOR01000159.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_1438"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3388 a         1852 c         2045 g         3159 t
ORIGIN      
        1 ccgtaactca aactcgtttc tcatgttaac gttcctcata ggcgggtttg ggtctaatat
       61 caatacagtg gtaaagagcc tgcttgctga ctgtgtggag tcacagtaga ctactctcaa
      121 tgataatgtt ccctagggtt gataccaggt tttgttgcgg cttgattagt tgtggccagc
      181 ctgacttatg aacacggcag tcagtaacat gagacaatac ggctaggata tgataaatac
      241 gtatgcggaa gagaagccaa acaattctag ttgcaataac aggaattgta tcactactac
      301 catacatcgc cttacagata gtaggaatgc aatcagttct tattgtaatg ctttcaggta
      361 taactaattc tcatacggtt caggaaatct cactcttagt tgcatttgtg atactagctg
      421 ccttcacata taccagcgga ctaaggggag caactcttac tgcaatctta aaagacatac
      481 tgatctggat aactgttgtt gctgttataa ttgtagttcc tctgagtatt ggcggttttg
      541 gtactgcctt cagggatgta aagccaagtt acgtgaccgt acccaactca cttgttactg
      601 gatatgcaac tctggtattg gggagcgcgc ttgcactgta tctttatcct cacgcaataa
      661 acggtgtttt gagctcagaa agtgtacata aacttagaag gagtactgca ctcctaccgc
      721 tttacggaat aggacttgct attatggcgc ttatgggaat tcttgtatat gctgttccac
      781 ctgcgatgaa ttttctttca cattttcctg aaagctcacg tggtatactt gttgttcctt
      841 cattgatttt gtattcaatg ccaccttggt tcacaggtat agctcttctt ggcatattca
      901 taggtggtct ggtaccagca gcaattatgg caatgtcaca ggcaaatctg ctcacaagaa
      961 atataataaa agagatcagg ccaaacacgt ctgcaagttc tgagattaga ataacaaaaa
     1021 tttcatcaac tgtcttcaag tttgtcgcgc taggttttgt gtttactatt ccagctactt
     1081 acgcaatatc actacaactc ttgggaggaa tacttattat acagatttta cctgcagtat
     1141 tctttggtct gtatatcaaa tcattaagaa aagaagccct cattcctgga ttgctagtcg
     1201 gaatattttc agggactttt atggtcgagt atacaaacca ctttggagca ctaacgtcgt
     1261 ctctattcaa tataccaatt tttggttcat tgtatattgc tgtcgttgcc cttacattta
     1321 atctgacaat atcatttggc ggaagcatca taatgaatag aaaagatgga ctagctttgt
     1381 ttcaaaccaa aaaacagtag gaacacagaa tacatatttc agaagaaaaa agaccgtgca
     1441 gcgtatattt aatggatgga gttgaaaaat tagaatttgc ctctcacaac tccaaaggat
     1501 taatggtgag ctaaggatgg ccctctatta acaagtttat cacatcgcat tacctgcagc
     1561 tggtttattc atcatgccac cagtggaagt tgtgttacct gatttcatgg gcgaattgcc
     1621 gctcgggttt cctgccttcg gggtagtata ttgttgagcc agtaccgagg cagcagaacc
     1681 tgaagttgcc aacataatac cagtagctac agcgatagcc actgctaggc tattatacgt
     1741 cattttctcc attatccatc gagcgtagac ctgttcttta atcctttcat gtctaaaata
     1801 tgaagaactt gtcgatatcc ggtccaagaa acaacccacg aaaagtgaat ctcgtatact
     1861 cattgcatta ctactaatag tgtcaataaa ttataatatt atatcacatc ttgcgctaaa
     1921 ttatctttaa ctatttaacg aaatatgatt ctgttgattc gagcccccat agagttgacg
     1981 atgtaaacaa gtaagaatat gggggactcg gtgatcttta cgtgtctgac gttattatgt
     2041 tgagatagtt agttatatcg aactaaacct ccttaggtca ggaaggattc tactgtgata
     2101 gatggaaggt actatctatg ggtcttggtt ttctaatagt acttttctga agtagtcgtc
     2161 tgagttcttt gctccgtttt ctattcatag gagccaggat ctacgaaacc ctgacgtatt
     2221 agtctgaagt attcctcttg ctcttttctt agacccctat gatctctttt aggatccgag
     2281 taaaacccaa gatatgtatt aaacacacat tacaaatcaa tagcgaattc attattatta
     2341 aaaaaagaaa ttatcttaaa cacagattaa tgctattatt acatatatcg aagtttttac
     2401 aaaaaaatta agagctgcgg atgagacctc taatcttctg tgtcaactca ggcgtcatgt
     2461 cttcagcttt tatattatgt tctgtcgttg catgttcgcc ggccttgctc atgatttcgt
     2521 cttctgtatc tcctttgata accgcagcac agttaaatcc tgcatcactg cacgttaatg
     2581 ttttcattat taacaagaga gcattctgtt atttaaataa atatgggatt gagcagtatc
     2641 gaaactcgtt tttgaacata tgaaacagaa atgaatatgt aaattactcc atcttcgcat
     2701 tgtttgacat ccaagatctg ggttcaattt ggatgagatg tggataacaa ttggaaaaat
     2761 tacttttaaa tacttaaacg gcagggagcg cattgacaaa gagtaataac aacaagaaca
     2821 acaaaaaagt ccaaataaac atgtggaaga cgatgagcac caccgctatt ttattatcag
     2881 cgattgcttt tgtttcatcg tccgcgttaa caagtgggag cagcattagc gtgcttgcag
     2941 cacaaagcaa cggcactagt agcagtagta gtaatcagag aatgacatta gctcacacgt
     3001 tagtccaaaa tctaacaaag gcaaatgtgc ctgtaacatt gcctttaaca agaggatatg
     3061 ttagtggttt cgaggtattc tatatatcaa cggaagcttc agataaaggg ctagcagatc
     3121 acttgacaaa ctttactcat tcaagagtat catatacacc agcattaaag aatgcaccac
     3181 cacaatcact cagaaatgtc tatgtatttg ggaatggcat taaaggatct ggtcctttag
     3241 gttttcaacc taacgtagct gattctcagc caggcgatcc agcatatagt cccttttgga
     3301 gaattaacaa tgtagagtgg aaacaaggga ttagtcaaag cgagctaaaa tcagaagctg
     3361 atatactatc tgcacagaaa cgtggagaat taactataac tccttcagat gcggtagtta
     3421 attgtccttt tgtgcagtgg catggcggtt cgttgaaaga aagaagtgac agaactttaa
     3481 ctgatctgac ggcatatggt ggaggacaag ttctgaacat cgatataaag aaaatgcagc
     3541 tcacgtttgt aggacataga ggatttgctc ctgatggttc aacaatctat tatatagcaa
     3601 ctgacgcatc tgtcaagaaa gtagctgatg cattaggcgt gtcatttgta aataagacag
     3661 gtgctgcctt gctttctgga gcatcgtctg atctcaacgt gtttacaaac ggaatcaaag
     3721 gcactggacc tatgggattt caagcaagca ttgcatctgc aaatgtaggt gacacagcct
     3781 acagtccatt atggagaata caggctacaa catggaagga tctatcacat actgaattcc
     3841 tgacttcatt aactcagact atatctgcag cgcaggctgg aaagctaagt attagcatcg
     3901 caggagtagt agtcaactgt ccgtttgttg tagtacatgt ttaggagtaa acacagaaaa
     3961 gaaactagac agaggagaga aacaaattcc atctcttttt tctttctttc acttttgatc
     4021 tgatcatgac aacaacaact ataagaatag atttttcact taaaagagaa gtcttttccg
     4081 tagtaatagg gggcgaactt tacgatataa aatgtagaat tagaacagat atctgtagat
     4141 aaaccattgt ttaaataaac tctatgggaa tggcgcacat ttggtattag agtcgattcg
     4201 aatattcgtc gtagtatact gagtcttccg ataaaagatg gtaaggaaac atactatccg
     4261 ccccgatatg aaccatgtgt gcgtgactat agataacgat ttttactggt tccttagtta
     4321 cctcagtcac caatgcattg tgaattacta aatacaacta ttctatcaac tgaccgactg
     4381 tctgccatct cacggttaat ggacaagggg gtacgaaaca atttatttcc taaatacata
     4441 tcaccgatcg tgtctaggta ccgatgtcca gcatgtaatt ctttcaactc ggtaaatttc
     4501 aacaagattt ttgatgggcg gttattattt agttgttcaa aatgtaagat ctgtgcatta
     4561 gtaccgaaac attctgacgc tgacagtcat gattctgcat atcttgaatt cctagatctg
     4621 ttcgacaatg ggcgtatagc caaaagcgat gatttgaacg caataatgca gcaagaaaga
     4681 atcctaaggc cgccatcaga aattaaaaga ttactagaaa gcaataacaa taagaaagga
     4741 acaaaaaata agttacttac ggaggtattg tattctcagc aagattatgt ggtagattat
     4801 agagtaattc aaggaccaga cggcaaacaa ggaagcacag tcgatgacct acctgtagat
     4861 gacagaatta tctctgtact atccagaaag gggattaagc ggctttatac atttcaagaa
     4921 gagtctgtta acaaaatttt acgtggaaag aacacagtta tagtagcacc gacagcatca
     4981 ggtaaaacag aagcattctg tcttccaata ttgcaaaaga tatccgaggt tatatactcc
     5041 tcacatttgt cgtcacgtca ggtctttgga gtatttgtgt accccaccaa agcactctca
     5101 agagatcaac ttccaaaaat aagagaattt gcagaaagtc tggggattcg tgttggcata
     5161 tttgatggag atacgccaca caatgaaaga aatagcatat tagattcggg tcctgagata
     5221 atcatcacga atttcgatgt tatacattac catctttggc acaaaacaaa attttcatac
     5281 ctactcagaa cttctcgatt tttggtagta gacgaggccc atatttatac aggagtgttt
     5341 ggagccaatg ttcactacat tattaaacgc cttgaaagat tgtctaggaa taaattacaa
     5401 gtaatagccg cttccgcaac actaaaaaat gctggtgagt tctgtggaac actttttggt
     5461 agagaaatgg aagttattca aggcgaaggt agaaaaggaa agattaattt tgtaatgtta
     5521 tttccgtctt tacgttccca cagatctttg gttttggacc tagttaagcg aacaactgga
     5581 ggaggtcata aaacaattgt atttagcaat tcacatcttg ggtcagaact tttgtcattt
     5641 tatttatcta gacagggaat aaaaattaaa gtccatagag ctggactctt accttctgtg
     5701 cagaaaatag ttgagcagtc atttaaggac ggcaaattga tggcaatttc tgcaacacca
     5761 actttagaac ttggaattga tatcggtgat gttgatgtta tcatatcaag cattgtcccg
     5821 atcaacagac tgacacaaag attcggtaga gctgcaagac gagggcaaga gggttatgct
     5881 attctagcac taggaaatga ccccattagt cagtattaca ggtcacatcc tgatgaatac
     5941 ttggctgatc aggagccggc atataccgac ccttcaaatc cttttgtaga ggaatatcaa
     6001 atacttgcaa tggcttgtga taaaccgatt tccaaatatg agttccacaa tgaaaatagc
     6061 gggaatgtga gcaatgctat tcaaaaactt gtttcaaaag atctcatcag attatcaagc
     6121 gatgaaaagt tttttgtacc taactttaag aaagcatatg agaaactaaa tgagtatagc
     6181 atcagaggga ttggtaataa agtagatata ttcttcaata aaaggatagt tggacaacga
     6241 acactcccac aagcaattca agagcttcat caaaacgcaa tatattttct atctggtaag
     6301 aggtatgaag taaagaaatt gcattatgat gctcaacaga cagaacaacc atattatgcg
     6361 gagctaatgt caattccagc tgattatcag tattatacga gggctatggt tgacgaatgg
     6421 ccaagtatta ttgaaatcta cgaacaaaaa aaagtatttg gaatagaggt aaaatactgt
     6481 tccttgaaaa ttcagaagaa agtcgtcgga tatgccaata tcgagatcgg acaggaaatg
     6541 acccaaggaa agaaagtaat gttctcagaa cctattgaat ttgaattcat tactaaagga
     6601 tttgtattca gagcaccaaa accaattgac attctcaaat atacaaatga tgaggattat
     6661 gtagaaatga gtggctatca tgcatcagag cacgtactca tagagggaag tagtatgata
     6721 acaggaggag cttcgcaaga tatgggagga gtatctcttg gctcttctgg tctgattttc
     6781 atccatgatg gcagtattgg tgggaatgga gcgagtaggg ctctatacga gagatttgat
     6841 aatgcgataa gaagggcatt gagaatactt atcgaatgcc catgtaaagg tgaaagtggt
     6901 tgccctagat gtacgtactc ttatagatgt gggaacaata atgaatattt gcataagatc
     6961 gcggccatag aaatcttgaa caaagcagca tcgggacaac aaacagaaat cgatcaaaac
     7021 gctcaagctg accgcgctct tatatagtaa ttaaacgacc gtcagatgag actttatttt
     7081 tcttctataa taaattaaat agaaaaatag tctaaaatta aatactgaaa atattagtaa
     7141 taaaaattta tgtatctttc atatttattt tgaatcatgg gtaatataaa aagccttact
     7201 aattattagt aacgttaata cgtcaaagac gatatgttaa tactagatat gcatgagtac
     7261 ctgcgagata cactcgtgta caggtataga gaatgtaaat cttgtacaaa tagaactgga
     7321 tttacatgca taaagtgtgg cttttgctgg agctgtcatt ggaagatgga acaggcggaa
     7381 aaatttgaat tatattataa accactcatc actggcacat ctgtgccttc ttttttggca
     7441 attattcaca atcaattgtg gatgaagaga aaagactaca gaagcagcaa gagtcactag
     7501 agaaactaag tcctacaaca acgaaggtaa ttgacgtatt tggtgaagag gcagaaccaa
     7561 tttgtgacta ccttagatgt catcacaagc tttcagtaca cggcctcgga actcgtgtat
     7621 gtcaatgtcg gcatcctcgg aacatcgcga tcggagcata gaaaatgcat actacgacaa
     7681 aagaaagtct acctatattc attgccttta tctccaatta tacatgtctc tttgagttat
     7741 tcgctgccct tccttgtcag acgtaagaat agaatattga tatgagaata attgatcgta
     7801 ctactgtgtc cgctgcgaaa atagctaaga ccatattagt tgttgacaat attagtttgg
     7861 aactacgctc cttatctagc atatgaggca ttacttcttt ggtgtttagg tatccaataa
     7921 caaccactaa tacttatgct aatcatatca ctcagtcatt atccataaat cattgatcct
     7981 gtttccggtc caacgaatat ccaatagaat tcgacaccat aattcttagt ccaattctga
     8041 ctgactgttc ttcatattga actttcctta ctaaggtgcc acatcactca tcttgtcgat
     8101 tttttgagca aaacctatta ctctttatag atattagata gtagttttga gtatgaaagt
     8161 gacaacaaaa acaacatcaa taggacaaat gatgaccgaa aagcttgaaa caattgacat
     8221 atcaaattca gctcaacaag catctaagaa gatgagagac aaaaatgtaa gttcactaat
     8281 agtaatcgac aactacaaca agcctacagg tatagtaaca gaacgtgatt tagtacgaaa
     8341 agtatgtgtt aatgatgcaa gtagcagtag tatgctaata aaagatatta tgtcttcccc
     8401 attaacgact attgatgcgg tatctccagt tgaagtagcc gcggatatta tgatacagaa
     8461 tagagtaaga catttgcttg ttgtagaaaa tgatgatata gacaagccct taggtataat
     8521 cactcctact gattttgtag gttatttaaa agaaaatatg aatattgacg atgtcaacgt
     8581 aaagatatta gaatctctaa aagaagaggg acaaaaagga gaataccaag aagttatcga
     8641 agaattagaa caacaagggg aactaccaaa ggatgtacaa aaaggtggag aacaatatga
     8701 aaatgaagag cccagacagg tatagataaa ttaaaagatt aagagcgcaa tgaagtctga
     8761 gcggcttatt gtcgagataa aaacttggga ttcgttcctt gttgttgcgc tacacacaag
     8821 agatggaaaa aatctggcga tccaagcgaa actaatactg ttgtctgcag tattcatttt
     8881 gactgctttc aaagaacaat atgtactttg actgctttca taactgttat cgcgtcattt
     8941 tgtgcaccga gcattaacgt atattctcct gaccccaaat ctgtcgcagg ggtgaagatg
     9001 aaggaaacct cctttgtatg cattggttct agtgaaaaag attgttcact aaaggatcct
     9061 gtggaatttc aaattagata tcgaagtgcg tactaaatct tataacaaga cagcttcgtt
     9121 atacttttgg ttgctaatat gtatgaaaag ctagtttcag atatttttat attcttcatg
     9181 tattacttct actgaaccgg cattcctata ttgctatttc cataaatacc attctaggac
     9241 ctcttgatga gattgtattc tagaattaag ttttcctata attaaaaata gagaaataaa
     9301 gaagtggaga ctctatcttt gctgactagc ggctacgctt taggtgttga gaatacatct
     9361 ccataaatag agttagcata tttcatatgc acacattcat aaataccgtc actaaagcca
     9421 cccacatcgc ctcagcgcct tattacacct ttttgagata agtctagcat ctttgcttcg
     9481 cataacgaat gtttgtatga atttgcagta atagcataac attgttggag agatggaaca
     9541 aacccaattt ctattgctac cgatagtgat acagcaataa tgaggattgc taatgtcatt
     9601 tttctttttt tacttggaac tactgtagcg agtggttcca agacttttgt tgatgttcgt
     9661 tttgatgatg tcactatgta gttttcacct ccttttgaat actgatgtgt aatttcggca
     9721 atgttgttga tagtactgat cccagtgcta tagtctaacg atgagtaact gttttgtgca
     9781 ttgcatcctt aggtttttgt tgtgattata aaactaggga tgaattccaa ttgtggattc
     9841 caaaattgga attgagcatc ccatttttat atctcgttat tgctataagg accagcgtct
     9901 taataaaatg gagctgcata aaaattgata tggatacaga ctgaaatact ggttactcag
     9961 tctatcgttt atttcatcgc agctgctatt cccatatatc ttacttttat agtaaagaaa
    10021 aagattggtg atgataacaa tcacttcaag agattaacaa tagtacttgc aagttttgtt
    10081 ctaatgcaag gcttttatca tgttgcaggt tcttttggct ataagttatt agcaaaagga
    10141 atactagaac cgttgtcgtt tggtatacta attttctttg gagtattcta cattattagt
    10201 aagactagcg caaaaaatga agacgaagtt agagtgacat aatatgatag acgtccagag
    10261 taatatcaac ttcatgtcct atgcaggtgg attgacttta gttattctct tctcgtcatt
    10321 ggctatattt ggatggcttg cagtcagata tagaaatatt agaagctttg aatttcaaat
    10381 ttctgtattc atcatattct acattgttgg agaaatacta gaagattata aaattccttc
    10441 tctt
//
LOCUS       DAOR01000160            2548 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_19602, whole genome shotgun sequence.
ACCESSION   DAOR01000160 DAOR01000000
VERSION     DAOR01000160.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_19602"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          759 a          518 c          535 g          736 t
ORIGIN      
        1 atattttatt gatactgtac tcgactattt aagccaataa cttttctcta aactaacatg
       61 ggttatgcat aacttcataa tattttatgg atatgtatca ggatattttg tgcaaattct
      121 tccggtttag tttggacacc aggtctgcat agttgataag tgtctcctgt aaaagccatg
      181 ataaactgtt gcaataagta ctgattcata aaacaaatag aatccatccc aaaggttgag
      241 taggaaatca attaaatatg taagttcacg tttaagtggt gccaatgagt gacaaccacc
      301 agactctcgc tgcaaagtgt ctgacacaac tcgaagaaat tgatataagg cccggtaaga
      361 ttttccgaaa tctcacggta ccagaattac tagaggttgc cattcataga atggagggag
      421 agttttctga taccggggca ttatccgtga aaaccggcaa atttactggc agatcgcctg
      481 acgacagata tattgtcgat gacgatataa cacacaatac gatagattgg gggaaggtta
      541 accatcctct ttcagaagac aagtttgaaa tgatttttgg tagaatgaga acatttgtcg
      601 aaggcaagga gttttttgtt ttcgacggtt tcgttggagc tgatcatgaa aataggctac
      661 ccataagggt aattactgat aaggcctggc aaaacctttt tgcgactcaa atctttatca
      721 gcccaactga tatagagcta gtaaatcacg accccaagtt cactctccta tccgtaaatg
      781 actttggcgc agaatatgac ttagaaggaa caagatctga gacatttatt ataataaatt
      841 tcaagaaaaa ccttgttctg ataggttcaa catcctacgc aggtgaaatc aagaaagcta
      901 tgttttcggt aatgaactat cttttgccac gcaaaggcgt ttttcccatg cattgttccg
      961 ccaacatcgg cagaagcgga gatacggcgt tattttttgg attgtcgggt accggaaaga
     1021 ccaccctgtc ggccgaccct gaaaggcaac ttgtcggtga tgatgaacat ggttggtctg
     1081 ataatcgaat cttcaacttc gaaggaggtt gttacgcaaa gtgcataaac ttaaggaagg
     1141 aaaatgagcc acaaatatgg aatgccataa gatttggcgc agtaatggag aatgttagtg
     1201 tagatccttg tactcgagag ccggactttg ccgatggaag tctcacagaa aatactaggg
     1261 ctgcttaccc tctcaacttc atcccgcggg cggttatccc tagtgttgca aatcatcctg
     1321 atgtcattat atttctaacc gcagatgcct tcggagtgat gcccccgata gctaggctta
     1381 cgaaagaagg agcaatgtat cactttatgt ctggttatac cagtaaactt gcaggaacag
     1441 agagaggaat cacagaaccc aaggagacgt tctcacaatg ctttggtgct ccgtttatgc
     1501 cattgtctgc taaggaatat gccaagatgc tcggacaaaa aattactaag catgatacca
     1561 aggtgtatct cattaacaca ggttggtcgg gtggcccata tggtgttggt aggagaatga
     1621 atcttgcaca tacgagagca atggtaaggg ccgcattgaa tggagatcta gaaaaaggat
     1681 cctttaccca cgatcatatt tttaatctgg atatcccaac gtcatgtaca ggagtacctc
     1741 cagaagtctt tgacacccgg aacagttggt ctgataaacg aaagtacgaa atatccgcaa
     1801 ggaggcttgc atcactattt gtcaagaact ttgaaaagtt tgataacgtt gctgtggaaa
     1861 ttagaaatgc cggtccaaca atataaatga gcaaaagagt agtagcccgg agcagattcg
     1921 aactgctgtc tccaggtttc ttttctcaca tgagatccag agcctgaaat ccctagccct
     1981 atcttggaga ttggccacta gattcggtac ctgattagat taataataca ggttcgaccg
     2041 ggcttcacgc gagctacatc acatccacaa ctagacccgt atttgacttt aacgttttgt
     2101 gttgatttct ctaagtacct gtccgtattc caaatatact ctcttccgca tataaggaat
     2161 gagcttatag tgtatagaat agatgttctt ttctctgata agtatacctt tcaccaataa
     2221 tgagacaaca ccacctgcga cctttgatgg tcgcatcttg tacatttgac aaaattgatc
     2281 tctctttaca tgatattctt tcaagataaa atatgacctg ttaacatcta atattagagg
     2341 ccaaatgatt ttctcccata ccattctccg attttccgtt gaagatgcgt gctttccttt
     2401 acctttaact ctgctctcct ttgcactggc ttcaattgaa ggcttcgtgc tcattttctc
     2461 agtatgaaac cttccaccag tgcgttgcgg ggcacattta aacgccggga tttgcaacct
     2521 atgtacatat ggaagctgat aattctgt
//
LOCUS       DAOR01000161            8440 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_104835, whole genome shotgun sequence.
ACCESSION   DAOR01000161 DAOR01000000
VERSION     DAOR01000161.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_104835"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2799 a         1460 c         1692 g         2489 t
ORIGIN      
        1 atgtgtgtaa gtacaacaat tagttaagcc ggaagaacga attcccgaac cgctacggct
       61 tgcagatatc tactccaaag cgcaagcaaa ttcggtacct taaacgtaac ccattacatg
      121 tgagcacaat cagaaccacc gacagcaagt cggacttcat cactagtgat acggaataag
      181 ctttatttgc tactttttgc gatatattgc attgtctttc acatcacgca cagggcttct
      241 gcaaacggca cgaatttcct aaattgatct tactttcttc tctcgttaat ttttctttag
      301 ctaatggatg aggtccagta gccgtactag cctttagcga gcaagactac aggctaatga
      361 agaatacagc atagcctctt tatatagaga attcccattc ttttcctatt cttgtctgtt
      421 tcagaaaact cacaacaaga tagtacggag gtgttgtacg gtgttgaaag tgctgttgga
      481 agaggcgtcc aattcatgca aaatgcaaac aaatggatgg atctgtatgg agatgaaaat
      541 ggcccgtcca taatcattga atttcctgat atttacaaga ataactatat tgaggctaga
      601 agaagaggag ctaaaataag attcatcaca gaaattacta aagagaatat gcgttactgt
      661 aaagaactga taaaaattgt cgatgaattt cgtcatttgg agggattcaa aggcggcata
      721 gcggttagtg agtccgaata tatgactact accacattac gtcagagtga gatcctgacg
      781 caagttttct acagcaacgt caaagaagtt gttgaacagg gacagtatgt ttttgatact
      841 ttttggaaaa aagcagttcc tgctgagcag aagataagag aaatagaaga gggaatagta
      901 cccgatttta tagaaccaat caaagatgct aacgagattc aaagtcttca gttaaaactc
      961 gtaagctttg ctcaagatga gatattgata atatttccta ctgttgacac ttttcgttat
     1021 taccaagaag tcggcgttat tagatccatt ttagatggat tgaacaaaaa tccaagtatg
     1081 aaagtgaata ttctaactgc taaagataag aatgtaatag atatagtaga tagacttcta
     1141 agacaacatt ccctccataa cttagatata aaatatatcg tccaaccagc tgaagccacg
     1201 gtgttaacac tcgttgtaga taggaaattc tctttgtctg tagaagtaaa ggaacaagaa
     1261 aatagtcagg aacccataat gggacttgca acatactcta atagcaaatc aactgtattg
     1321 tcatatgcat caatctttga aagcctctgg agacaagatg agctttatga gcagttaagg
     1381 cattcgactg aagatttagg tgagatgaag cgatatctaa accaagtatt acgagaggta
     1441 aaaagaaaca agtgatatag catgagagag tgtataattt ttagatttgg ttatttctga
     1501 tagatgagag atgtaggtgc tttgatgcag ttgctattca cgctattact tccgctacca
     1561 cttgaacttc cgcctatgac tatgtaccat tgttcttgtt atgctcagat cgtaaggatg
     1621 gtatgcccca aagttacagt aggagataaa aaatatcaaa ttgaaccttt caatcctaca
     1681 gtaagttcat tcagaatagc attgggatgt agaagaattt gcaggtgcaa tgataagggt
     1741 cctggaacag ccgatggaag cctttgcaaa aggaaggagt gcttatagca atgggtcgag
     1801 tcatacaata agttaacgta gccatgccag ttcgggaaaa cattatttct atcaaacgct
     1861 aataattatt aatggatatg tcttcaacct gtgctacttg cggtaagaag actcaagatt
     1921 atccgatttt ttgttatatg tgcgacgact acttttgttc agaaaattgt gatcttcaaa
     1981 aacatgccaa accatggcag aaatattgat gcagtcgccc cagcgaagtg ttcaaatcat
     2041 gatatcatac cataaattat tattgaagag tagggaggtg tagtattata atgcaagctg
     2101 cagtagttcc agcaattcac ggtaaatggg aagttaaaga tgttccaact ccacatccag
     2161 ccactaatca ggttctgata aaaatacatg cgagtggttt atgctacact gatgttcata
     2221 tcacagaagg tcatttgcct acctccgctt ttcctcgtac tataggtcat gaaccagcag
     2281 gtgaaatcgt cgaagtggga agtggtgtca ctagtcgcaa gattggagac agagtaggag
     2341 taccttgggt acaagcttca tgtggtagat gcgaatggtg tcaacgtggg aagccaatgt
     2401 tttgtgcgca gcagataggt actggtgtca atactcctgg tagccatgca gaatatatgg
     2461 tagcttatgc tgatacgaca actcttcttc caaatggaat atcatatgag caggcagctc
     2521 ctgtattttg tgccggttac actgtttgga gtggtttaag gttcgctgat ccaaaacctc
     2581 atgagaagat agcagtatta ggaattggag gtttagggca tttagcaatt cagttctcca
     2641 aggccgccgg tttttacacc attggtataa cgcactcaac ggataaagaa gaattagcaa
     2701 ggaaacttgg agctgatatg gtagtaagta atggagacgc attaaagcga gctggaggtg
     2761 cagatgtaat acttgttact agtaattcgt acaatgctgc aagtgaagtc ctaaagggtc
     2821 taagacctga tggaagatta gtattgatgg gtgtttcggc cgaaccgctt gtagtaacgg
     2881 ctgaaataat aggtaaccgt agccgcatta ttggttctac acaaaacggc ccagaatatc
     2941 tttatgaagc attagattat gtggcacaag gaaaagtcaa aataattgcg gaaacatact
     3001 ctcttgatga tattgctcgg gcatatgaga acgtagctgc aggaaatgtg cgttttcgtg
     3061 cagtaataaa aaccatctag agacattcta tcttgattta ggtatcatat ccatctagtc
     3121 gtgactgtcg cacatatcaa cctccttgaa aagaggtttt caatgcacag aatgggctca
     3181 cattataaca aggaagatag tagtagtcat tctatggttt tagatgtttc catgtcgtat
     3241 tctgcaatcc ctgcataccc gcaaactaat gctagaaagg taagaaagac atctggagac
     3301 atttctaaac ttcgcagtga ttagtacggt aatcacaaca gccatcttag cagctggtgt
     3361 gatagcagcg gtgaagccta caactcaatc agcatccaca atgactatga tgccagggaa
     3421 ccaaacgggc ggtaagatga tgtcggggaa tgctacgagt agtatgatga agggaaatga
     3481 gactacaccg cccatgttgt ctaagaaata atatcaataa attttgacat gaagtagaga
     3541 cagatctagg aataacatct cctaaaaatc aacaactcac ccaccacttt tttttacatc
     3601 tggagtacaa gttatcttag ttgtttatat tccattacca attgctaaga atcttcccgt
     3661 tgttcatccg aggaatcttc gtcctcgtct aactcatcca aatctttctc gaaatcatcc
     3721 tcgagcgggc tatcggagtt agtattttca gacatttctg atactttgcc agctcggaga
     3781 taattcaatg aagaattaaa ggatttctat taccctataa ttgccctata attgtgtcaa
     3841 attctcttac gtcttatgat cccatcatga caggaatttt aaggggagtt ctataatcct
     3901 tagtaacact taagccctga tctgctgtaa gtgctgtgac tgttactgat ttaagtgtag
     3961 aattttctgg tggcgaaagc atagcatttt cacttccaat cacactaagc atcaactaat
     4021 cattgatgtt aatggttaac agcttacaag cggagacata gcgtctggca gctctggatt
     4081 gctttcattt aaaccgggca ttgtctatac catcctacaa caatgaaggg aataatccaa
     4141 tccaagtagg gtgatcaaca aaaaggagta tagcagattt actatattgt attctcccaa
     4201 aattttttta atccaataag aggtcgatcg caatgactaa caaaaggcct cctaggtgtt
     4261 acactatcat tcatggtgga atgaagggtc agatgacgat gatggctgat atgccaagaa
     4321 cttaatacaa acttgcatga ttcccatggc cagagcaata actatggaac aaaaacaggc
     4381 atttgaagct gggcccgaaa ttaggagttc taacacctat atgatagtca aaaatgagag
     4441 atcaaaacgt gccatccttg ttgctttgag tgagaccgag ttacaaaaga tcttggatgc
     4501 aacgatgttt aattcgaaat cagttggtca aattatacga gatactgaaa taccacatac
     4561 tgctgcttat agaaagatcg ggtggcttct tgaagaaaag ttgcttgtag ctgataaaat
     4621 cgatattaca gaagatggta agaaatctag tttattccgt actgtactaa aatcattcaa
     4681 tatcaagtat gaatataata atgtaattat tgaagcagaa cagaattttg atactttgaa
     4741 aaagattaca gaaaggttct tttcactcga taactgaacg cgatgaacga tataataatc
     4801 agtgtatgta ttgtatgaac gaatcatctc gtagattacc aagagacagc gttgctactg
     4861 tttttttgaa tctttcttag tctttccaat aaatcagtta tttgttgatg agttggaata
     4921 aaagccttca caatgtcaat atctacatta agtttctgta attcgttata tacctcttta
     4981 tcttctatag tatcaattaa tttgtttata ctaaatggtt tttgcattag ttctactact
     5041 tgttttagtt gtttgataga attttctaat atttctttta tatgcgcaga agcaaatatt
     5101 attctctgat gtgaatttac tgcaagtatt tcttttgcta cttccatacc gttaatttga
     5161 ggcatcttat agtctaataa tactacatca aagggtaatt gaaaacgttg tatactgcgc
     5221 gtatcagaag agatcacttc ttgaaataca tcatgataac ttttgagaca atcttcacca
     5281 tttgaagtta ctgtaacctt atgatttctt gcctctaaga cctttttata aagtaatgca
     5341 atatcctctt catcttcagc tattagtata ttcatatgat cactgatacc ttaataccgt
     5401 aagaagagta atatgataag cacatttaat aaattataca ctaatccttt ctttctttta
     5461 tagcagttga atttaaaaaa ttaatcagat tgtaaatgag aatttatcta ttgccaatga
     5521 tctgatgact ataacaaatt atgcattaat aagacatgaa tttgactttt ctgcggttta
     5581 tgtagccgaa gattcaacgt tgcatcaatc aattcgaagc tttgccgtaa aggaatcgaa
     5641 attgctatat agcattggaa tagcgcccat ggaagaagag atacagaaga gtcatagaac
     5701 tcacaaaaca aaatcaagat agtatggtag aagagtttgg aattgtcttc ccttacagaa
     5761 gaggatatga aacagtattt acaggaggta ataaatggag tacagaaata taagactact
     5821 accaacaaga ccaacaatgg taatacaaag actctagaag catggcagga cgaaaatctc
     5881 aaattaaatg tgctttatgc ggcaagacca tagtcagtaa tgaaaaggag aaaccaactg
     5941 aaaaacaaag aatcgtggaa ataatcaatg gtacaagata ccccttgata ccgtcgttct
     6001 ttccctgatt tcacctgtta ccattgcata ctgctttgag agtaaataca gtagcgaaaa
     6061 gagcttgtaa aggctaataa ttttgtttaa attgctgtaa atctattttt aggatagtgg
     6121 aaagtcaaaa tagtagttgc gattctcgta tccatgctta caagaatgac aagacaatgt
     6181 agcagtgtag acaggaagca agggttattg tgagaagaaa tctcaaacac gggcgagatt
     6241 tcatcgaaaa gagtattgca agtaacggat taagacgaac tagtctataa attaacttta
     6301 aaatatcgtc aaaaaggcta tgatattagc aacagcacga ttccccacgt aaagaacact
     6361 tgcatgtaaa cgcttttttc ttgcatttcc ttaagagtct gaagtgcatg accaacagaa
     6421 aaattgcttg ctaaggggtc atttaatttt aaataaaaat aatttatatg agattaggaa
     6481 agtcagatat gaaatgcttc gaccactatg cgtcagattt ttgtgcttgg catatgtgac
     6541 ataaaaatgt agagaaaacc tagaatgaat gacatgagta agaaattcta ccaactggct
     6601 cctgacctga caatatgcag aatagccaat ggcatgtggc aggttgcagg cggacatgga
     6661 tatatcgatc atgaattggc aattgcagac atgatgaaat atcatgatgc tggctttacg
     6721 acttgggatt tagcagatat ttatggccca gcggaagatt ttattgggca gttcaggcgt
     6781 aaattatcgg cactaaaagg aaaagaagaa ctcaatagaa ttcaggcgtt aacaaagtgg
     6841 gtgccacagc caggaagaat tacacgttct atcgttaatg aaaatataga aagatcgctc
     6901 cgcagaatga gtgttagctc gctggattta cttcaattcc actggtggga ttataacaat
     6961 ccgtattaca tggatgctct caaatgccta tccgacctcc gggatgatgg aataatcaag
     7021 catataggtc tcacaaattt tgatactgaa cgtatgcaga tcatgataga ttcaggtcta
     7081 caaattgtat cgaaccaggt tcaatattcg atcatagatc gtaggccaga agtaaagatg
     7141 ataccatttt gcctagagca taacatgagt ctccttacct atggaagcct ctgtggaggg
     7201 ctgatgtcag agcgctatct aggaagaata cagccatcta ctgccgagtt aaatacgtta
     7261 agcttacgaa aatacaagca gatgatagat gcatggggtg gatggaattt gttccaagaa
     7321 cttttatcga ctttaaagag aattgcacaa aagcacaatg taagcattgc aaatgtagca
     7381 acccgctata ttcttgccaa ctctgcggta gcaggcgtta ttatcggtgt tagacttgga
     7441 atagttgatc atataaacaa taatgtgcag gtattcaact tttgtttgga taagtcagat
     7501 tgtgatgcta tagatgctgt atgtacaaag tcaaatgact tgtttgagac aattggagat
     7561 tgtggtgatg agtatagatg atcttgtgct atttgcagtt cattaacatt ttggattctt
     7621 atttgagcag taggtgaaat aagataagcg tggtatagca agaggagatg aattaggagc
     7681 tgaaacagta ccatagttca ttgaatttca agtaacagtt tcattgatct cttacttaga
     7741 aggcgatatg actcaatagc agtttaattg tacatgaatc caatacgatt taagtaccaa
     7801 tgtttgagaa tttaaaatat cacggcatat ggtattttag caaagattaa acaattccat
     7861 gcatttactt cttggaattg ccgagactca tggcgtgacg gtggtattga ccaatcaagt
     7921 tcaatcatcg ccttcttcaa cgggcactag cttatcaatc atctttcttc cactctttct
     7981 tacggagtat agtcttagcc ataatattat agctaaaaat actgcagccg ctatgtgaat
     8041 ttgtgagcca atataaggta atgatgtcga aagagaagga attttatgaa cttctaatat
     8101 ttctcctatg atatatacca caatgaacag agatatttga aactcaaaac ttctaatgtt
     8161 tctatatctt attgcaagcc atccaaatat agctagggtc atgaaaagaa tgattaatgt
     8221 taatcctcca cccatagaaa acaagaaacc aatattgatt tgagtggata tcattgtact
     8281 tctttaacct cctcctcttt cgctttcgac ctattaataa gatagatgac tccaaagaaa
     8341 agtagaatgg caaaagacag aggttccagt attcctttag ctaacaaact aaaaccaaga
     8401 gcaccagcag aatggtatat accttgtatt attacaaagg
//
LOCUS       DAOR01000162            8005 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_70220, whole genome shotgun sequence.
ACCESSION   DAOR01000162 DAOR01000000
VERSION     DAOR01000162.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_70220"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2560 a         1510 c         1435 g         2500 t
ORIGIN      
        1 tctataggat atacacttcc tagcagaagt gtaacctaag tctgtaagtg aatttgttac
       61 ctatgtgtgt aagtacaaca actagtcatg aatcgatcct gtgttttata gatatattag
      121 taaaatatat tggattgctt ttgacagaat ctcctcttct gtatcctcct ttgattacag
      181 cagcacagtc aaattaaggt ggtccaaagg ttatactcat aaatgatatg ctacacgata
      241 ttttgctcag acatcatagt aaagcataag ctcttgtgca atcgatatat ctctagcttt
      301 tgctaattcc tcgtatttga gcactatata agtctccagt aattcgtcgt gaaagcatct
      361 ctttaagtaa tctgaatcgt tcttcaatga ttcgagtgct tcttctaagc ttcctgggag
      421 tgattttatt cctaaactgt ttcttttagc atcggacatc ttgtagatat tttcatctgt
      481 tggactccca ggatctatcg tcttcttgat gccgtcaagc cctgcagaga ctattgctga
      541 aaatgccaga tatgggttag cagctggatc aggagctctg aattcaatcc gttttgattt
      601 agcattattt ctttcattaa taggtatacg tatgacagta gacctgtttc cttttgccca
      661 ggcaatatag actggagcct caaaaccagg aatcagtcta tgatatgagt ttatcgttgg
      721 agctacaatc gcagctaagg aatgagcatg atcaattaat ccacctataa aatatcttcc
      781 aatttgactt agttcagcgt aataatcatc actatcatag aagatgttag tagttgatga
      841 ctcgttccat aaactaatac tagtatgcat acctgaacca ttatcaccac catttaaagg
      901 gtcattttta tcaaaaagtg gttttggcat aaaattggct actttattgt acttccttgc
      961 gacattcctt acaacgtcct tgtatatctg aacactgtct gcagcctttg taagcgcact
     1021 atgcataaag ttaatttcta tctgaccgca acttgctact tcgtggttta ggtttgtaac
     1081 atttatagaa aagtgttttc gtaaaatctc agcaacatca aatctaaatt ctagcaaaga
     1141 atcttgaaat ggaggtgcat catatccacc cttccttcta attgggtact tgcagccatc
     1201 acctccgtat ccctctactg ataaaatttt gggttcatga tattcactat tatcattaga
     1261 tttatcaccg ttaagatcat gattattatt attatgatta aaaatgatac tgtcaaatac
     1321 aaagcactca acttcagggc ccatttgaca ggttagatta ttttctatta gaaatgcttc
     1381 catgcgttgt gatacatatc tgggatctct taccaacctg cctttcttga aaccctgata
     1441 tacatccgcg ataactgcaa atagcttgtt attgtaattc gaagatagtg ggggtggcga
     1501 tgctgataat aacgacggag gaaagatcct tacagtagac ctgtctggta cgagtagcag
     1561 atcagaatca tcgatacttg caaatccttt aatagacgaa ccatctactc caataccaca
     1621 tttaaaaaag ctatcaaaag tttctttgtt ttcgaacacg tactttgcta gaaacttgcc
     1681 gaggacatca gtatacctta actgaatgta acctgtatct ataatcattg ttatgccatc
     1741 aattccttgt tctttttctt ccggttctaa tgcggaattc cttctactaa atgtcatcga
     1801 ttgacactag tatatggatt tagtaggata taacatttta taggtattat attaatatat
     1861 gataagtatt tattgacaat accttttatt tgtcaattgt caatgaattt aatagcaatg
     1921 gacgaaacag atgttaaaat actcggaaag ctgctttcag atgctagact ttcatataga
     1981 agaatagctg atgaaatagg cgtctctcct cctacagtgc ttgctagagt aaaaaagcta
     2041 gaaaaagata gcattatcaa attttattca gctatattag atcatgagaa actaggttac
     2101 gatctaactg caattattga ggtcacgtct gttaaaggaa agatagcaga agttgaaaaa
     2161 cacattgcca agttcccaaa cgtctgtgct gtctatgata tcacaggttt aacagacatg
     2221 atagtaattg caaaatttaa gaatagaact caacttagca attttgtaaa aaaagattta
     2281 gcgctgccat atgttgatag gacaaataca catgtagtac tgataacagt caaagaagac
     2341 tttcggtttt tctaggattc aaatatagat gaactgagta ccgatataga aatttgtatg
     2401 tctgttacat agcaagctgt tcgattatcg gttctgattt ttcctgatgc ttcatataaa
     2461 ccgttgactg ccaccaactg tatatttcct cgagtctgtt actcaacgta aaatgaacat
     2521 tacagattat tgggaccctt tatgaaattt caaatgcaca tcatggcttc ttatatgctt
     2581 aacctagatt gtaagagaat attcaaagaa atagcattag aatggagagg gggagacaaa
     2641 tccaactttg tcatgggcgt gatcagaaga gagaatgtgg gagaattcac cagcactatt
     2701 gtcaggaagc tcacgaccaa gtaatatcgg ggattaagcc ccccctttca tgtgtaaact
     2761 cttttcttaa aggagtgtta ttgagtataa actactcttc atgcactgac taaccagctt
     2821 ctcataatat atttgagcca attctgataa tttttggcgg taatagttat tctgcggact
     2881 agtaatagtt attctgagtc ctcttccttc cacgacgtct gctttcaaat tagttcggtg
     2941 gtatgagaga caaatttgac ccaattttgc aacccagcat atgcacaatt aatttgggat
     3001 tttcgctttg tcatttttca ctctattggc actaaatgcc cacgttgact taaaaagggt
     3061 ttgacttaca aaattaccag atcaagcctc tttctttttc aatagtcgaa aagacatgac
     3121 aaaaacagcc aatattactc cagcgctaat tagaaaagta gctcccccaa ttgaattata
     3181 caaaaatcca cccaaagtta gtccaacgat actcgccagg cctccaaaac tacctgcaac
     3241 gccctgtact gctccttgaa gttttgatcc tgcgcgtctt gacaatatgg acatgaaaga
     3301 gggccacata agaccgttac ctactgcaaa tagtattata gcgccgccga ttgagacaat
     3361 attatttgat acaaataaaa taaaatttgt acctaaaata atgctaccaa tgatgactaa
     3421 tttttcctct gagaattttt ttaatgcttt acgtaaaaca ggaccttgga tcaaaaccat
     3481 tattccactt aatactgcat aaaatattcc caactgtgtt actgaccact ttagatcatt
     3541 agctgcatga gtcggaaagg cagcatagta gatattaaat ccaagaaata tcaaaaaata
     3601 taatataagt aaaaaagaaa tatgttttag tttgaaaaca tctcgcaatc taggtttttc
     3661 agtgtttact gtcgaatatg gctccctgca ttcctgcgaa aaaacttttc tgatggttcc
     3721 ttcttccgga actaatattt cttctgacga gggcttggat tctcttaaca gaaagcctat
     3781 tacgattaac gtgaccaaag atagaattaa tgctgctaga actggcaata ttgctccata
     3841 gatagttccc cctaaaattc cagctagtgc aggacctaga ataaacccaa ggttggaaga
     3901 tatcgccatt tttccaaagt ttttactcct agttctatct gatgaaaggt ccgcaagata
     3961 agcattggca atagagatat tcccaccagt aatgccgtca atagctctgg caagaaatag
     4021 gataagcaaa ggaagaataa tgacaaatgt tccaaggaag gcgatatcaa tgctaaaaga
     4081 tttttcaaac ggcaaaaata atgcaaaaag aaatagtatc caaccaactg atgttcctac
     4141 attactcaat agaagaactt ttttccgtcc aaaaatatca gaccatcttc ctaatactgg
     4201 agctccaata agttggaaag caggataagt ggcggcgagt agaccataaa ctatggcatt
     4261 acctccaaat ttcaagacta aaaaaactag gaaaggcaaa acaatactaa agccaagagt
     4321 acctacaaaa ttaaccaata atataggaaa aagtggaggc tgttttacta gactagccat
     4381 tatttaggta actatagggt tccatttaaa aattaaggac acaatctgac ttcaattaat
     4441 tgatgctgcg aatacctcac tctcgaaaag tcctgcttat aagatagctt ataaattcag
     4501 agaaggcctt gatcaaatca aatcccttgt agttttaact ataaatgttg ataaatctta
     4561 tagtaattac tttgtctcag aagcgtccaa gtactcttct tatctgccga tagtacaaag
     4621 gattattgat tcatttgaaa tagacactag agacagaaca ccacgataac acaatagttt
     4681 gttttgttga tatctccatc cctcttggat ctaaccatct gccaatcata ttttacaatt
     4741 aaagttaaaa ttatagattt tggctgatgt catgacgtta tttatgttag aatagtagtt
     4801 aaagttgatt acctcagtat gcggaattta tgtcagatga taaagttctc attccagtat
     4861 aatgaaatag tcggttgatg ttgcagatag tatgcggtag gccacgccta agtttcagaa
     4921 atacttccaa ataatagtat tatcaatagt cgtagatttc tgataagcga tcaactctga
     4981 aaatttgttt aatcgtggtc cttggccttc cgtctttaaa cacactttta gattcgtacc
     5041 ttgtagagaa agtagctatg tccgccaaga gtggttctag agccatacct ttgtcggtga
     5101 gtgagtattc agtttgcacg ggtctactag ggattatcgt tcttttgatt ataccaaaat
     5161 cttcaagctc acgcaaccta atcgaaagtg tctttgtatt tattccttca attgatccta
     5221 aaaattgacc aaatcttttt tgctttagca ctaccatatt tcttactatg tggagtgcat
     5281 atttttgccc aaaaatctta agactgttat caacagggca ggccaccatg ccaaagtttt
     5341 tcaagtgttc ttgtaattct atcatattcc ttacacgtaa tattaccatc tgataactat
     5401 taaggttata tacttacctt tatatactat atgtatggaa ttcatagaaa tgattggatg
     5461 gattgcattg ggattcgtac ctacacttgg gatactggaa tacacaactc gaaaacaggc
     5521 taaaagaacc ttagtaagac tattcgtaga aagatagtct actaatactc ctaaccgtac
     5581 tgctgcaact gtgcacgaat atgcgacaaa tgttagtgac gcatggaaca ggttattagc
     5641 tcgacaaacg gctatgtacc tattcaagaa atcgcccatt aatttattgt atattgatta
     5701 caatactttt tccgattgcg atcatagcca taacatcaaa gttatctgga atgtcaagat
     5761 cttttcttgc ccttccataa tcaaatcctg caatcttatg agcgactaag cctcttgagg
     5821 ttgtttctat tgctagattc tcccatgcag ctccagtatc aaattgacgt gttgctgatg
     5881 caatgatcct tccattatat ccaaattcct tttttgagac tacaacaacc aatacggcgg
     5941 catttttagc ccagattttg tcaaagtcct ccagtaagct gaaaagtgta ttccactctt
     6001 ctgtatctct tgttgcataa gtaaatatcc aaggttgaga attagcagaa gatggtgccc
     6061 atcttgcagc ttcaaatagg ctcattaatt cttcatagcc caatttctca ccagtcattg
     6121 atcttggaga ccatctattg agaatgattg gatttatttt ataggttgag atcctatctt
     6181 cttccagaac tgactgtgac aattgactac cccttatttt ttctaccatt atcgtattaa
     6241 cgagcaggaa ttacttactg acagtagtaa taacctacta agtaggtaca caatctcaaa
     6301 ctggattagc tcatagtgca actacagtaa tatcgtccca gcttagttag gtatgggtca
     6361 ctaccataca tatataccgg aagctctaat atcgtccatg tcagaggtag aagttcgaca
     6421 gtcagttgta ctaaaaccag atactactgc acctgacttt actcttcata gtacaccaga
     6481 ccaatcggta tcattaagcc agtttcgcgg taatccaaca gtccttgctt tctatcctgc
     6541 agacttctct ccagtatgtg gggatcaaat gacgctatac aatcaagtct tacctgaatt
     6601 ccagcgtttt aaggctgaac ttttagggat atctgtcgat ggtgtttggt cacatcttgc
     6661 cttttccaaa gacaggaatc tccattttcc attgctttca gattttgaac ctaaaggcgc
     6721 agtagctaaa aggtacggtg tatatcgtca acaagacggt attactgaac gagctctatt
     6781 tgtgcttgac agtaatggaa tcatacgatg gagttacgtc tcactgatag gagtcaatcc
     6841 tggagctgaa ggaattctaa aagccttaga atctctctcc gaatcaaaat aacaaggaga
     6901 taagaagaaa aattgggcat gaagacagag atcgtaaaac tgactttacc tgtaagcagc
     6961 gatcgcgatc atattcaagg acgtacttct gctcctgtga cattgataga atatggcgat
     7021 tatgaatgtc cctattgtgg acaagcctat ccaataatca aagaagttca aaagcagctt
     7081 ggtaataaat tatgctttgt ttttcgcaat tttccactca ctgaaattca tcctcatgca
     7141 cagcatgcag ctgaagcagc tgaagctgca gcagtacaga ataggttttg ggaaatgcat
     7201 gattatatat ttgaacatca acaggcactt gatgataagc atttagagaa atatgccgat
     7261 aatttaggat tgaatttagc caagttcaac aatgaaatgt cctcccatgt tcatgcaggt
     7321 cgtatacgtg aggattttct tagtgggata catagcggtg taaatggtac accaactttc
     7381 tatatcaatg ggatacgtta caatgactcg tgggatgtag aaactctgtt cgaaagactc
     7441 agatctgcta tttagaacgc tagtaagaat caaacatcag gcaatatcac atattagatg
     7501 gtttcaacca aatgaatcta gatatagaat aagatcctag atcctattcg ggaataatta
     7561 aatctaatat agaagaggtt aaataattat aaacttaata ttcgatagat gaaggcagct
     7621 atttttgaaa aaccgggttt agaaaattta aagataaaac atgatgtaga acaaccaaaa
     7681 ataacagatc acgatgtctt gataagagtt gaagccgcag gtgtaaatcc aattgatcat
     7741 tttgtggtgt caggcacacg cccaattaaa ccaataccgc atataccggg agcggagaca
     7801 agcggtgtga tagaagaagc tggactgcat gtcacgagct tagagaaagg tgatagagtg
     7861 attgtgcata acaaggtgtt tgatggaatt tgtgatatgt gtctgagtgg aaacgatatg
     7921 ttatgtagaa acggaggatt gataggtgtt gctactaatg gaggctttgc agaatacatc
     7981 actgttccgg agaaaaatgt cttta
//
LOCUS       DAOR01000163            5283 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_69881, whole genome shotgun sequence.
ACCESSION   DAOR01000163 DAOR01000000
VERSION     DAOR01000163.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_69881"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1551 a         1029 c         1119 g         1584 t
ORIGIN      
        1 gattttgaaa ctaatattcc aacctatgca ggtacatcgg caacagcccc agattcagta
       61 gctatgccct gggataaaat tattgacaga aaagttaaat caagcgacaa cgaagatatg
      121 ggtaaggtag aaagtatagc tactagttat gttgaagtaa aagaaggtgc agtccataaa
      181 aaacgctatt ttattccaaa atattacatc caaggatttg acggggagaa cttgctggtg
      241 tcgcttacaa aacgtgagat aaaagataga tatgaaagag atagcccacc aactgaatct
      301 gagtttcaaa ctcaagaata tatagaacag aagcgtaaag tagattccac ctatccacag
      361 tttttacatg gaatcccttt tatggcaaag gaatcaggag taaatcttca aacagaacac
      421 tctggtgaga cactcaatat cccatgggaa gagataatcc acaaacgtgt gaagacaacc
      481 gataatgtgg acataggaga tattgaacaa gtaggaaatg aatttattgt agttagagag
      541 ggagtagcac gaattcgtca ctactacata ccaaaatcat acatcgataa ctatgatgga
      601 agttccgtgt atgtagctgc tcccagtgga ctagttagtg ccaaatttga gagggaaacc
      661 gaaccaacac cggaagacat tagaatatta gcaaacgacg ctccacctag aaaagcctca
      721 taaagcatag cttaggttag attacgtccg tcgcaatgta agtgggactt gagtctcgta
      781 agcaataatc cctatcgtcc ttttttatta ataacacctt ttccaagcta ttgtcgatgc
      841 attaaatatt ccaaactaaa tagagaatac tagcattgtg attatgttat cctacaaatg
      901 agatttcaga atcacaacgg taaactccag aataaaaagt atttccaatt taaagcgggt
      961 tgagttcgca gccaaattac ctagtacgac tagtagcagt agtgtcagta cgttatgcgt
     1021 aatgaagcca acgattcagc cgacatacaa atcttgatct cgcagccatt gcaacgccag
     1081 aagcggctgc attgaataat tgtgcatggg ttccgaaaaa taccgccggc tcctatggag
     1141 agaaaaatcg gaattggcgg gtcttagata aagtaattag atatgctgtt tttactatcc
     1201 taacccactt aaggctgctg cacttggtga gctacttgat ttacctgagc cgtgatgatg
     1261 ggtatggcta gtagagctat tatggtgtgt ggtgtttgca ccattactgc caaagtcagg
     1321 tgctcctgtt actgctggta tcgctgaatt acctgagaag gtttgggtgt aacagttcac
     1381 aagcccatcc tgagttagtc ctccaggacc cttggctgca gtagactcac acgcaaagag
     1441 gtttttcaag tctttggtag gagaggcggt gcttttagga ttagtgcctg tctttacagc
     1501 taacagggaa ccagcagtga gactggccgt tacaagcact aatgccgtcg ccgacacaac
     1561 ggcaaagata gccaattttc ttgtatacat tgctaattgt ggcaatgctg ttatatttta
     1621 tatgaagtgc gtttaagaat attataacta accttatgat tagacatgct ataataatta
     1681 ctaattctat ccttttaaat tatctctact gtcaccataa ctaagctatc ttattatcaa
     1741 taaactaatt atcaatcttt ataagcaagg ccttttttgt attagcacct catttcttcg
     1801 ttgctgttgc ttattatcct tatcattact tgaattggaa cctagagtag gcgatacgat
     1861 gaatttcagt ttttacaaaa aacaacaaca gacgggtgga agggaggatt acatctaggg
     1921 aactgttatc gttttcaccc cgagtaagct ggcttataca tgggaaattc ctggtatgcc
     1981 taactcaaaa agtgtcgtaa cgtgggagct tgaagaaata gacaataata agactagact
     2041 aaacctcatg gatacaggaa ctaaatcaga taaaatgctc aaagaggctg atgggggttg
     2101 gacttttttc ttgaatgaat tagtaaagta ttgtgagaaa acaaaataaa acaaccacat
     2161 ctcttttttt ctagacaatg tacatcaata tcctgatcgt ggagaaaagg tctacagcca
     2221 tttcgatcca ggccatcata ctcattgcca atcgtagctc aactccacga actttggttt
     2281 gcctatcagg tcgctgtgtc cgtctccaag tattgtaaaa gtagctctct ttcctgtatt
     2341 acgggctgta gactgtctgt ataaccgtaa gttacgccta tcagtttatg tgttgaatca
     2401 taccaatgtg ccgttatctt caggttttgt atagtggcat tgccatagcc ctcacctttt
     2461 atgtagtagt ttcctatcga gtctggtcct gttgggttaa cgaaatctag ggtcagtgtt
     2521 tttggatcgt tattcgcaaa gtcacttgca ttagatggca gcattattat tctgtcgcta
     2581 ctggcatttg ttgtttgtac gagtcccagc cctggaatgc ctcctatgta tttgactgtc
     2641 gtagtggtgg tgcttcctcc ttcaccttcc ccaccagcgc cagactttgt tgctgtcgga
     2701 catgctacta ctcctgtgat gtttgcagtg caacccttct gaaatgaaca taattcgtta
     2761 ctatacactc caaatccatt accgcaatat tgtttgttgt caagctgcaa cacgccgtta
     2821 acgtagttac ctagaagatt aggaccacca cacgtatcca ttttgtcgca atctctacca
     2881 gctttcatcg catcgtcaag tcctgcctga tagtcatcat attgtttggt gcctggtttg
     2941 tacggattac ctgctgctat attgaacgct tgtatatact ctaatgtgtg tggaccaggt
     3001 gcagcgccag cagcaatttc agtcacctta gcaaatcttg gtgttgtttt ggtaggtgga
     3061 tttcctgctc cggtattcca tccagcacaa aagtttacgc tgtgaccgct aggacaccta
     3121 cttcctagat tccctttgcc atcttgaaag cctatgctat agcaagatgg atagcctgat
     3181 tgatcgcaat gtgcaacatc tgcctgttga gaggctgacc ctgttgggtt attactgact
     3241 gagctcgaat tccctttttg agtggaagag cttggctggt tattattatt agccgtggca
     3301 gttgcaccag aagcgtatgc gtctggcggc ctaatgtcat tggttgccca cggcgcacaa
     3361 gatgtctact taacaaatga tgatgatgct cttggtccga tagtccctgg gcttgacaac
     3421 gacacaaatc aacactgatg ttgttgttga agtgctttgt attttaaacg tgagacatgc
     3481 atagatacag tggttagttg atgagattcg ttactcgatc tctttttgag taaaaggttc
     3541 caaggctaaa tcaagcacgt tttttgatct taacgaattt agtggttcta atgaatttag
     3601 cagtttggag tgtttgaaac aactgattgt gcacctatgc cccacaacct ctaaacccta
     3661 taatcctaac cgtaagtgtt gaaaacaaca atacagcgac cccaaccacc agtacgacaa
     3721 acccaacccc catcaacaat tttcctattt gcggacatat gagttgcaaa actgcagaag
     3781 cggaaaaggg tgttatgata ctgagtgtaa caattgataa cctctttcta gttttaataa
     3841 cctctgcctt attcgaatcg gaagaagata acgtatacaa gattgttata aggatcagaa
     3901 ttcctgcaat tacggtagca tcagcttgga gtatgtctct aggatcaaga accactgcat
     3961 tatacatcct cagggcggca cgttaaatag atttaaaagt attcttctta gtttaattcc
     4021 gccagaccag tgttttactg tataaattta aatctatata aatttaaatt taatcctcag
     4081 tatctatctc gtatatccat gccgctgaga tacagaccta tacgtatgag ggatatttcg
     4141 tcttggtctt ggtacattaa ccctgttgtc gggctagtta tgacgggttt tgatctagta
     4201 caacggatga ataaggagat gaatagggag gaagcgttct actgtagtgc tcccgcacag
     4261 tggcggtatg gactttgctt taaggatgtc acaatcgaag atagcgtgaa agatgaagat
     4321 attccgtata tgtgtgagcg ttgtgggcag tattttgact ggcttggtat tctgacaaag
     4381 ttagtcaaaa aatgtcccga atgtggcagg gaaataggaa ttcaagaccc tatcaacttt
     4441 tgcaatcagc atggcgataa aagagtgtcg atacggataa gggaagtcag gatgtaggca
     4501 aaagggcctg tgttcatggc caatttgcaa tttattacta attttttgat tggcatggga
     4561 tagagtgagt gaattaattg ggaaaagcca aaagattcct tattacagta ttcacaataa
     4621 tcgcaatcat gggaatcata tcattcgcag cgtcatatag tagcctccaa acttccacac
     4681 aggaagtaaa aaatgctaat caggacgtta aagcaattgt ctaatttata agagtgctct
     4741 atttaatgtt agtaaggttt cggtgttaac attaagtcca caatttatac aaaagttaag
     4801 cgaatacaat tctcagtgta ctgcactaac gggaagaaat gccgagtagt tagttttgaa
     4861 actctgatct cctgatagtc ccttagctta cgaattatgg taagttggca ttcagttgat
     4921 gtcttgcgtt tgggttcatt tggaaaagaa tcgatataac aaatgttctt cttcccgtct
     4981 ctggaatatg cctacctgga ttttctttaa tatcatcatt gtctagaagc acttgaaggc
     5041 atttgagcct taaagtcggt aataagcagg ttatttatcc tcgtgggagt atgcagctat
     5101 ccagcgtcga cgtggcatta ttcataaaag tggcctcgtt tagacctgcc gcatgcttga
     5161 agactttatc aattgagcta tgtagaactg agtgaagaca gttcataact ttcgattcgt
     5221 tcattaacat accaaaacca gcgggcggtt gttgttgtgg tggtggcgat tggaattgtt
     5281 gtt
//
LOCUS       DAOR01000164            6462 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_33751, whole genome shotgun sequence.
ACCESSION   DAOR01000164 DAOR01000000
VERSION     DAOR01000164.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_33751"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2193 a         1116 c         1252 g         1901 t
ORIGIN      
        1 agactcccaa gaaatatttt cggatgaatt attccgaaat tcattgaata gattccccct
       61 aagttcatta gaattaatct ttgcaatctc tactctggag taatctttac tgtcagttct
      121 gaaatccgga ttcacagtct ctttgaacat taataactga tcgtacaaga attcggtaga
      181 tccatattta atgtatcatt tatcgattcc atatatgtta tcatacaatg gattcttatg
      241 gtgatcgcag ataattagaa gcaaatctca atacttatta atcttggttt tcaaacctaa
      301 tcatgcctat agatccagaa tttccaagaa accaacaagt tattggcaga cataatcatg
      361 ctgatggcca acattatcac tttgtatggg gtcccggaag tatttctgat gctgcagcta
      421 atgaagacgt aagagcagcc tatgctgcaa gaaatgagga acttgttcct atcggtgtac
      481 atggtactgt ggttgcggtt gattgggatt catgtattgc tgatggatct tgtatacaag
      541 catgccctgt aaatgtattc cagtggtata gaacagaaaa tgatgtaccg gctgtagata
      601 tggctaatgc tacgagcgca ggaaatggga atactggtaa agaagataga ctagataata
      661 ctgacaaatc ttaccccatt agagagcatg aatgtatctg gtgtatggct tgtgttacgg
      721 tgtgtccacc gaaggctgtt aaggtagacc aagctaatct agaattccat caaagagctt
      781 agaaacgttt caatagtgag aaagacttca atagtgggaa ggactatctt cttggcaaag
      841 ctactttaat ttagggatat gcacaataga taatagcttt ggtaaaaaga taaaaaaacg
      901 ctatagcatt actggtagct atccgacaga acaatacaaa gaatttagac ggcatgagca
      961 aaaaatatcg gacttttatt actaaaatat atttataaat gtatttttta gatcatttgt
     1021 tgttgtatca tgaggcgtga atccgtgtgt cctatttgta aaaagaaatt gatcgaacat
     1081 tcggagttcc aagacaaaat atgtaaaatg attacgataa aggaattttc aaataactcg
     1141 ccgggttttg atgttcaatt tagacctttc ttcgaagatt agatactata tttttacttg
     1201 tatctcctgc tattggattt tatagcgcaa taaatacacc actatagtct aattagtaac
     1261 atagaaacag gccatattag aattgtgaga aatgctacaa tatcaaaata ttttatgata
     1321 ctgatataca tataccatgt gatgtatgaa tagtacaaga gaagaaaaaa tcatatgact
     1381 tgtaagggta tctgtatacg ccataaggca atgaagccac ttggtgttgg tcgttattct
     1441 actggacaga aacgttgtca gatatgcgag atcttcttga aatgggatgg tttatggtgt
     1501 ccatgctgtg gttatagatt aaggactagg ccacgaaact cgaaacttaa agctgaattg
     1561 cgagcaagca atgaaataga taaagctaaa tcattaggtg tatacgagca gccacctatc
     1621 caagctcatc aaaggcatat tgcacacaaa atgaaccatt gaatgcacta tgacatagct
     1681 ctttcagtac tattattacg tctaattcaa gctttctttt tcctttctgt tgtaattagt
     1741 tgatgcactc tagttaaaca taatggctaa tttgtacgtc cttgttataa ttacagatca
     1801 gcatagaaac tatattctaa tcactatagg tagatattat gagatatttc aatatcaaag
     1861 tgtttttcgt catttcttac atgttcatct tatttgcttc atagttcact acaacattgt
     1921 ctcaaatagt taatattatg tgtccttttt atgtagggca atatttggaa gaactagatt
     1981 tcctccatga attatgcctg actgcattgt tttccaatag atttgtgaag tttgcagctg
     2041 ttcttgataa ccatggaaaa ctaattggat catccgaagg gagcagcaac attgaaggtc
     2101 tcgaagctat ctctgatact ggtcaagatc taattgctaa acaattgatc ggttcactat
     2161 ggttaacgga tctaacgaca atgcgacttg agctcaatga tgtgcaagaa attatctaat
     2221 gaccttttat gataagtctt ctagcagaag cacgaacgaa agcagaagca tttcgggcga
     2281 cggctaaaga gtacattcct ggaatgtatg atgcccttag aagtgaggat cataatatct
     2341 cacccgcagt tgctagagat agaattcaga aggattgcat aaacatttgg agcaggcgaa
     2401 caatattggt tgctttacca gatgaagcaa aggatctgga aaggcagaaa gtggggcgat
     2461 tgggccaaaa gaagcgaaat tctgctgcat tttctgcagc atctatatct gaatataaaa
     2521 atgagatttt gatcgatact tatggaaaca ccgtagcaga cgtcattcca tataatcaca
     2581 gaatttatcg tgataatact gatgccgtta aaaccgcatt cacacctgca agtcaattct
     2641 gtaaatcgaa tctcatagag attgttcttg atttaaacaa gtatgggcaa caaatactat
     2701 ctctgatacg taacacgact agtacctgta tactcaaaat tgacaaggac ggaaaagtta
     2761 ttgatatcac cgacggttac ggcggcagta ttgcagaaca ggcttcggat tcagagtcct
     2821 cttgataaat cccgaacaaa tgtacgaata gcatctgaga gaaatgtacc aatagtccaa
     2881 tttgtacaag gccgccgtca tcgtggatat gtacgatcgg aacggataga agaaattgca
     2941 attgaaaagt gtaggcagaa ctggagggac ggtatcacct ttaatgattt gacttcaagc
     3001 gggattgcag gcagcaaaga acaggcacaa cgtatgttaa agtattcctg tcacaaacag
     3061 aaaaaccttt tcacaatgag taatagaaag ccccagctat actatccaaa tagacttagg
     3121 gggcatatac ttgagaatat caaaaagatt caacaatacc acaaacagat tcatattgtg
     3181 acaaatgcca cgtacaaatt aataagccaa tcttgatacc atgatgagta gtttctatat
     3241 gttttcatat gaagatatta agatacgcca actgaattta ccacttgttt ctattgtaaa
     3301 aatgatgtat ccaaagtgag tccttatgat gtttggacta tgcaccaagg acaaacatac
     3361 tattgcttag tatgtaagaa actactagaa actgtttgga taggaccgaa tccatatggc
     3421 aataactaag aagaagcaga agataataga taaggaatgg ttcccagtaa caaggaacaa
     3481 accagtcaac caataaaatc agatggagat gcaaccttgt gtatacaatg caatgctaat
     3541 actgtttatt caatagctgg ttgtgccgtt tgtactattc caaacgaaga atgtagacgt
     3601 aggtattccg gtaaatgact gaatatacag gctgggataa ctacgacaaa tcaaagactg
     3661 accaactaga gaagcgaatc aaagaccttg aagaatggaa gcgacgatat actgaggctt
     3721 cagttcctta tcaatgggta gataagaaag tttgggatga tataaaatat caagtatcaa
     3781 ggctagcaat ttatttgaat agatatgaag tagataaact tagaagtgca atcagggaag
     3841 cagatggaaa ttaactgccc taaatgcaac cataggtttg atgctagtcc gttagaacgt
     3901 gaaatcataa ctaaatgggc tatagaagaa gataagaaat tctttgcagc tatgacacca
     3961 gaagaactag aacattacaa tagaacggaa agagagtcag gattctaatg actggctaca
     4021 actatgaaga tttcatgaaa gccgcaatat ctgatacaat gaagttcaca caagaaaaat
     4081 taaagacaga acgacatact gaactataca aaattctacg tagaaaagca ggtaggcaag
     4141 gcgtatggac tgtaatattg gcctacgtta tggatttgat cacaatacat gaagctttag
     4201 acttggtatt tggagataaa cactttctag ataagacagg aagagactat atggagataa
     4261 taaatgactg acaaagaagt gataaagcaa gcaatacgag aagtcatagc agaggcggag
     4321 gagatgcaga aaaacaaccc gccgtccttg tttgggattc tctgtaagta ttgtttgaaa
     4381 ccaatactct caggtcagat atttgccata tttcatattg attgttttga ggagaataag
     4441 aaatgactga ttgcaggcaa tgcgggaagc tactcgaaaa acgaaatgaa gtgttttgta
     4501 cttggacatg tctaaggctg tggaaattta ataacgtcgc tgtgcttggc agtgattgga
     4561 gaagaaattg actagccaat tcagagttct atataccttc aaagtcaatg cccggcaaac
     4621 aatacaatgt tatgctacat cgtggtaagt ggttttgtac ttgcgaacat gcttcattat
     4681 ggccaaagct agaaaactgt tcacatatca gagaggctaa gaataagaaa tgagatgcca
     4741 taagtgcggt gttgaaggtg atacaactat agaaatattt caggaaccag gtaaagagat
     4801 aacaaaatta acatgtcata gttgtaatga agagctttat tctgaaagga catattaatg
     4861 aaacattgta tttactgcgg tcaattcata gcgtttgacc ggggttacgc aagaacccgg
     4921 tttaaccaaa ttcttcttat cttccaaaaa cctaacaaag attagtctac tcatcaaagt
     4981 tataacaaac aacctcttag cattgtcatg agcatattgc ggtgctctca aagtattaag
     5041 gagacatttt tcatgcgtac gagtctttac gttgtaacca aacacataat gtatataatc
     5101 ttcataaaat ttggacgtaa tattctcctt tgcttcttca agattcctac tgaatgcttc
     5161 cgttgcatca ataatggtct ctgaaccaaa atattgatat tctgggtaat tatgacagta
     5221 tcggtaacgc agcacaacct cttactaacg ataatagcgc tcccaatacc ccatctgtat
     5281 tacatttgtc cccgggtccc agaaattata ctattagtaa taacaatgcc atctctgcat
     5341 catcaacatc tgacggcatt attgccgact caaatgctga atcatgacag cggttcaaac
     5401 agtggaacac atcacatcca tcatgacatt aacgttagca gaagcagcag caattatcat
     5461 aaagataaaa ataatttaca tgaccttgcc cagaaggtaa caaacaacgt taagcataag
     5521 ctcaacgtag gcgatatccc ttttccatga cacagcaaat tacattatcg gcgaaatgga
     5581 attgaaggga tttttataat atcatttgtg gttttttcaa cgggtaatat acttgtaagg
     5641 tctttaggaa ttatagatta gataaatata ttgagaacat gtcaaattga gatttcgtca
     5701 tatcagtgga taagaacccc gcatgtatca aataattccg tgcagttctc actaagatat
     5761 ctgaattatt tcccgcttga tgtactgctg ccactgcttc cttggctact gtgaaattcc
     5821 ttatcatagc agttattagc atcttgtaaa gtgagttgcc cagccttgtt agcaatttca
     5881 gtcatacaat taaagtaccg ttttaatgca aaggcggggg tgacaaatgt gcttgcagtt
     5941 ataagtccta tagctgctat tatagcaata atacttagat acgtatggat cgacttcatg
     6001 gtcattaaat aagtatgtta agatctttat aggaaatatg gatcggatta ctataagata
     6061 atcacgactt tgtttactgt tcgaattatt agataattct tctgtattta gaaacctgag
     6121 gttgcattct caaatcgtat atgcaagcca ttgattctta gaacgatgtc tccaaatacc
     6181 tcagtgtttt tcataggttc cgcaatcatt atctctttta agccgtcatt atttccgtcg
     6241 ttatttggaa caaatcgtga ttgcattcgt gcttaagcat tcaatagcgc aaagtaggcc
     6301 agctagaata ggaagccaat caagactttt cttttatgtt taagaatact tgacaaagag
     6361 gacacatgaa aattatataa tctcccaaat cctttctact ctctaaatct ttggcctgaa
     6421 atagttccgc ccatatactc gctgctggaa actcttgttt gc
//
LOCUS       DAOR01000165            2601 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_15427, whole genome shotgun sequence.
ACCESSION   DAOR01000165 DAOR01000000
VERSION     DAOR01000165.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_15427"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          787 a          499 c          460 g          855 t
ORIGIN      
        1 tctattgtgc tacaagctat acatttagct aacccttata atgtagtcgg tgttatcata
       61 cattacaagt atggtttaac gctccgtact ccttcatggc atttgcattt ttcttcctga
      121 tattccactt gcgatgatct caattcctaa tattaacagt gctattccta atatgaaaga
      181 cgcgagaatg gatacaaaaa ttgcaccata gagaggtgat gccattatca acccggataa
      241 agcaatagct ataacccctg caccgatgct aaatcctctt gcccaaccac gactttgttt
      301 gtctactact ccacgtatga tacgtgcgat accaacaaac agtaaagcaa ttgtcaataa
      361 aattatcacg aacacagctg tatggacagg atatcctaca gctatagaac cgaagatgag
      421 agataggacg ccaagtccta cggtacccca ccgtgatttg ttcgcgccca gcccaaatat
      481 tccagttata atttcaaata ttccaagaat gatcaaggtt atacctagta tgtatattat
      541 tgagaacagt gttatccctg gatgtactat aactaatacc gataatatga tggcaatggc
      601 acctagacca atttgcactg cccgcatcca gccgggagat cttgatttgt tcatcgatat
      661 actatgataa taacctctat ataaataaat gaaaaatgat ttcttaattc gtcttacatc
      721 ggatgttaaa ctagttttgc accggtgcat gacaatatag attagttctc aataaggaaa
      781 aggagacgag agaataagtt tcctggttat ttccgcgccc agaatgcaac tcgcttgaca
      841 gaacgtatgt ggagccgtat ttgcgagtga ggcttatcct aaaaaccaaa aatctggtcc
      901 cgtttaacgt agttacaggt atagtacttt ggtttgtttg cacccaaaac cgaaaagttc
      961 taagagatca cactgcctta acttatctaa gtctcaagtc ataaatcatg gttttcttaa
     1021 gaaatatacc ttcaagatct atcctcttga tcattacacc gcttgtttta tccgcttata
     1081 ttcatatttg gaatcctata ggatttcctt ccctatattt tgatgaggga atatacatga
     1141 gaagagctat taatatgttg gagactcata gccctcaaga agatccgtac ttttatgatc
     1201 atccatattt tgggcaaatt tttttagcca gtatattctg gatgactggt tatcctagct
     1261 cactacatcc ttctgtaaat agcatacagt ctatagagaa gctatattta tttccgagaa
     1321 tattaatggg agtacttgcc ataatcgata cttttctagt atataagata tcagagcggc
     1381 gctacggtag gaatgtggca tttatcgcat ctactctatt tgcagtgatg ccaataacat
     1441 cgttactaat gcgggtattg ctggatggaa tccagttacc ccttttttta tcttccgtat
     1501 tttttgcaga tcgtgtcaat atcaacgact tgaaaaacaa taatagaaga tttgttatta
     1561 ctctactttc aggcgttttt cttggattag ccatattcac gaagatcccg gtgtttacta
     1621 tggttctatt ggtaggattc ctagtcttta ccaataataa aaatagcttc aaggtacttg
     1681 gcatatggct aattccggtg atattggtac cgatgatctg gccagtttat gcaatatcga
     1741 ttggtcatct taatgattgg ttaaagggtg tttattacca aactcataga ggagctcaaa
     1801 catttttttc ctcactaaat ctctttttta aagctgatcc ggtttttttt attgttggta
     1861 tagcaggctt agtctttgca gctataagaa gagattactt tcttttgttg tgggctattc
     1921 ctttcttgat ggccctttac tttgtagggt gggtcctgat atatcatttc atcccgttat
     1981 taccagtatt gtgcattgca ggagccagat tgcttctgga aataccaaat agaatcagat
     2041 ataagagtat cgctaggata tcgctcgctg ttattgttgc tggaatactg atttttggaa
     2101 taaccacgac tttcttactg ataacatcca acgccaattc tttttatttc gaaggagctt
     2161 tatttcttac acagtattta gaggataaca atgttagcct caagaaaaat aatcttacta
     2221 taatttcaga tgctttttat ttgtggatac cgcaacatgt attccattta cctggcactt
     2281 acaaaacatt ttttgatagc acattatcta gaacccaaat gagcctttta atagtcgatg
     2341 caggatttat ggaagtcatg cacgaacata ataagcaagg tatgcttttg cacaccattt
     2401 atgacaccaa taatacaaaa aagatagcag tgttcgggga aaatcaatat aaccagaata
     2461 tatcaatata tctttataag ccaaaagctt attcacatta aaaggtctag ctccttgaca
     2521 agtgatccgt taacgttgat aagctaaaca agaatatctt ccttctgact atgatggtgt
     2581 tgttgacaga aagaatgaca t
//
LOCUS       DAOR01000166           10145 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4477, whole genome shotgun sequence.
ACCESSION   DAOR01000166 DAOR01000000
VERSION     DAOR01000166.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4477"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    7286..7552
                     /estimated_length=267
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2918 a         1944 c         1802 g         3214 t
ORIGIN      
        1 gctactacta ccgctactac tacccattat catatctacc attgaattct cacgattatg
       61 gtggccttga tcggtgtcct ttttttctac cttcaacgag tcctttttat cagtatcttc
      121 ttccacggaa tgcaaattga cgtaatcaac ctttatagtt atattagcat cttgagaata
      181 gtaactaatt atgcagtacg agaagactaa aagttgctcg gtcatgatgt aaacaagtaa
      241 cacagacaaa taatttggaa ttaactgatt gattataacc ttattatttt cgggaaaact
      301 tttcgctaat aatttatcgt catttattat taatccagtg tcgcatcgta tttagccggt
      361 caatggcctt atatttgatt gtgattgtct ccaagagaag gcctggattc agctgagaaa
      421 ttctcgcctt ttaaatcaac tgcggagggt atctaaccga cttgtcataa ctgataataa
      481 tataatcgat ccaaacctca ccgagttccc atattctacc tttgtaattc actctgtcat
      541 catatttcgt tggtgtagct ctttcgcggc attctttatg attttagcta catattcatc
      601 tagttctaga acacaagcca aatcagaagg agaagacatc tctttgattt catcgtttct
      661 gcgtttaaag gttaaaatcg tggcattaat aattcagagg tcctagtaaa agtgagttgc
      721 atatatactt acattagatt agctttctgc tatatagaaa ttcctcgacg cgagataatc
      781 tgatatgatc aaatgtagac gaattttgta agagttagct tgactgttgg tccttactct
      841 aatcacggta aattaaaatt actacgaaac agctgtaaag atttaggaac agtaatacaa
      901 tgatctggaa ttcgacaagt cacaatcgga tatactactc ggatagagcg tgcatacgtc
      961 atcttcgtcg gcgcaactat tgttagtact attattttca tcattactgt cgcttgtgta
     1021 atatttgtca acaatctgca acttatgttc tctcgggttc aagtctagat tagtaattat
     1081 tggttattag gaagttaata gacccgtatt ctagtcgaca ttattagtat caaagcttga
     1141 ttccttgatt ttgaaaaata attgtttctt gaggcctagc aggatgagat attatgtgaa
     1201 cagaaagatc attttcgtta agcatttcta catataattt aacaacactt ttccccctta
     1261 aaagtcggtt gctggatggt gactgaatcc agtctttact ttttttgtag cgaatcatgc
     1321 ttgtagcaag aataggaacc tgcaaagtta ccccgtgctt cacgagtaac atgaggaata
     1381 tcgatagtaa atggtttacg ttactgagtc caagtccaga ctcgatattg atttttttat
     1441 aatataagtt gaagaaagaa ttcacagaat caaaaattac aatacaagat tcagccattg
     1501 aatttatgac gttatttaaa attgattcaa agttgccctc ggtaggaagg taaatagtga
     1561 tgctcttgcg aatatcctct attattagtg gagtagcaac gcgtgtcgct gttgttcccg
     1621 ctgccgttac tatagtcaac tcctttaata acaatccggc cttcacatac gcactgaacg
     1681 tcgtatccaa gtctatgtaa cttactttat ttttaaaagt gtaaggggac cgctgttgct
     1741 cctgctcctg cactctgcca aggcaacatc gtgacgcaag catagcagta aaacaaagtt
     1801 tcgcgtaggg gtcgttaaat ataagtgcat tcaaacccgc gatgctgagg atatcgttat
     1861 agacaccgtc cacggttgta tattatggtg agcggaggca ggatatataa tggattatga
     1921 agcgttgcgt ggggatggcg gcacagataa aatcgatccc tatgacatta ttgaatcaga
     1981 ctctattcgc agagatttcc cagtcacgca caggaaggtt tacatgaata acggagcgat
     2041 cgctcctacg ccactgtcca cgatcaaatc aattactgat tttcttgtga aatcctctga
     2101 gggtggaccc ggttccactt ttatttccga atatataact tccttgatga aggagcttcg
     2161 tacgagaatt acacatctga taaattgcga acctgaagat gtaatattta cacaaagtac
     2221 aacggagggc ctcaattacg tttccagcgg tataaactgg agtagaaaaa caaatgacag
     2281 tattattgta agaggaggaa ctcatgaaca ttatgcaaat tatctcccct ggctacaagt
     2341 atcaaaaaga aagggtataa aactacacga gctgaagatt gacgaaaatg gatattttga
     2401 actggaggag ctagaggctc ttctcaaaaa gactaacaac ggaaaccata ataataaaag
     2461 taataggggt agcacaactt tgattacgtt gagccatgca ctttataata atggctcgat
     2521 aatgccagtt actgatgttg gtaaaattgc tagggaaaac caggccctgt tttgtataga
     2581 cgctgcccag acagtaggtt cgatttgcgt agatgtaaag aaaatcggct gcgactttat
     2641 ggcatttccg gcattcaaat ggatttgcgg ccctgtcggc atgggaatat tctattgcaa
     2701 taagaaagct gcagaatttt tggtccctca tttcatcgga ggagaatctg ccacactttc
     2761 agtagatgaa aaacaaaagc aaaagaaagt aataacctat gcagatggtc cggagagatt
     2821 tcaagctggt tttcgtaact atggtggcgt tgcaggaatg gagtcttcag ttcgttatat
     2881 tttacgctta ggtatagaaa atattcgtaa gaaaaattta aagatagcca atgttctgcg
     2941 agatgaactc gctaaaattc ctaatataaa aattcatggg cctgacgatg aaaacttgag
     3001 gacatctatt gtctcgtttt ctccggtttc atcctcctct acttactcaa gagtagattc
     3061 cagaaccatt gtaaataaac tcgaacaaaa cgagataata tttgctgaaa gagatatagg
     3121 aggtggaatg aaggcggtga gggcagcgcc ccactttttc aatacagaag aagaagcagt
     3181 gcgcgcagcc cgttatttaa aagacattct aaagtgatca ctataatgtg tctctcgtgc
     3241 ttgtggaggt tttatgctcg tttttgcgta gtcgcagtta gtgattactg ttatgatagt
     3301 cgatatcaga gatccagctg ctgtcttttg cctttctctc cttgaccctc gatgactatc
     3361 atggtcaaag tcgaccttac tttatcaatt cttcttacat gccacgtaat agtttctcgc
     3421 agtttgtcca ttgtttctga tttgatcttt agtataatat cataaacccc gtacaccccg
     3481 ctgacttcaa taacctctgt caattttttt aactctttta tgatttcttc ctcagaacca
     3541 agatcacagt taattaagac atatgctgtt ggcatcttat ataatatttt cacggcagtt
     3601 attaaactct tttcaatccc tgccttaaat ttagtaattt gctaaagaaa tatgacggct
     3661 tatactacgt gaccaaggct tcagaatcaa ccaggtctgt ttctgccttc cttttgtttg
     3721 ttcggagcca taaaactctc cctctatact atatacaaaa cgacactaca agcctatcca
     3781 acatgctttt gttattgtaa gcaaattata gacattttcg agatctgatt aatacttgtg
     3841 atgtcattaa tataacacac gattatgaag gtcatcgatc ttactcatga aatttccccg
     3901 ggcatcaggg tttttcctgg atctccacag cctgccttta ttaagtggtc gaaattcgat
     3961 acccatggct atgattcgga agtgatgttt ttgagtaccc atactggaac tcacatggac
     4021 gctccttgtc attttactct taacggtaaa agtatagatc agattgatgt taatagattt
     4081 atttgcaagg atgcactact actgaagatt gagaaagaaa caaacgagct gattactcgc
     4141 ggcgatatca tggattgcgg ggttaacatc gaaaagaatg atagcataat attcgataca
     4201 gggtgggaaa agcattacga caaaaaggag aatcaaaaaa ctgaacatta tattatggct
     4261 aacccgggtc ttggagcaga tgcctcacag ttccttgtcg agagcaaggt aaatgccgtt
     4321 gcattagata gtccgagtat cgatgccggt acagatcata attttatatc tcataaaatt
     4381 ttgttgtcta ataatgtttt agtaatagag aacctttgta atttagagaa attgagtagt
     4441 gggcaacggt ttacttttat agttaccccc cttaaactag tccatgcaac aggatcacct
     4501 atacgtgcaa ttggtatcgt cgaatgaatt acatgtcatg gatagaatca ggtatgtaca
     4561 cttcagttag gtattgataa tgtggatata gattattgta catcgaaaac catttctata
     4621 gcaaagattt tctgatacta gcaatgtctc tggctgaaca caccatattt tagatgatca
     4681 tcagtgaaga acatgaaaag tgcctacagt tgaaagatgg gacgcaattt gggagaaaag
     4741 attcgtccta gtcttgaaac tattgcaaaa aaggttgatg agaaaaagag agcgtatgct
     4801 gaatccaaat ccgttctctt gaattacccg cagctttcaa cactgcatca tgctccttgt
     4861 tgtttgcatg atcctgtcct gaagaaatct cctcaacagc tctctactcc tgactgaatt
     4921 tgcaactgca aaacaataag ttgtcataat ttatttatca ggtaaaatat ccaatctgaa
     4981 tgcctgtaaa gtcaattgag acaagaggtt catctccaac tatccatgcg tcaagcccag
     5041 gttgcactac accaccatcg ttatgaccac acattcactc tgatttttca ggaaattata
     5101 tactgagaat gcggagccat ctccgccctg gttcagttta gagtattcat tttttcggaa
     5161 ccaatcttag tatacctagt cccattatga tataacccac tattactaca gctaagaacg
     5221 aactttgaat ggctgtcagt cctcccttag ctattgctac tgctcccaaa accgacgtcc
     5281 cagtaactgc accacctgct ataagcaaaa tggttgttac ctttgacttt tctctaatcc
     5341 caatcccaga agaaaggaag aacaaaatta ttggtgatga gccaaatatc atccacgctg
     5401 aactgcattc attggcaaga agccttgttt cccttgtcct acagctgcgt cagtaatgta
     5461 tgccagtact aatgccatcg caacgattgc tagtacttca gaaattttct gtcttcgaac
     5521 attgtcttgt acattgttat attgttatct tataatatct tgtaattccc ttatttatcg
     5581 tagtatgcat ctgccaatta taatgactaa cataacttcg tgtaatcgat aacgtcctcg
     5641 tggatcatgg gtcttgcgag tctcatgctg tttggcctcc cttactctca gatgtcatgt
     5701 tgctcgtcag cacgtggttc atatgggtct cacgcctaac atagcctgct ttttctaatc
     5761 tactcacgaa ggcagcgatg gagccagtag taagacttag catattccag catctcgtca
     5821 ccaaaacagt cacaacatac aaaataatca attctgcgtc cctagatcct cgaagagagg
     5881 gagtgaattt tatatcagaa ttgccaatga tttttttaat ttagtacgat ggtgtttacg
     5941 cgttctagca gctctctatt ttcggtcggc tttgcgatga aatatctttc tcattcttta
     6001 ggagacactt cctttttaaa tgcttgataa tcttggaatt cgctaagggc tgtcagaaaa
     6061 cagactttaa ccttattgtc aatcttttta atttctagat ataattcaaa gccattcatc
     6121 tttggcattt tactatcgag aatgaataga aaatacacac tagctttgaa attcttcaat
     6181 gccaaaagag aatcattaaa cgaatcaact ctaaatccac ttccttctaa aacactctct
     6241 aaggctaaac taatatcagg ctccatctat tatcaagatt cttcttctct catcagaaga
     6301 cgttcctgct atcgctgggg tacgaagatc aacttcggaa ttgttgtacg ttaggtagcc
     6361 cctttaccat cattaggaaa ctctaacaga attatattat tgccacgacc atctagcatt
     6421 agataattga aatcgttgtt gtagttgtta ctctaccttt actgtgtcac tagttgcaca
     6481 ttcactcaat cactcactca cctttatgag atatccaaga aggcttcatc cagtaatctc
     6541 tcatcatttt tgcaacattt tcagaaaaat ctttgtatat gccaatccag ctaatcatac
     6601 tgctctgcca caatgccata ctactcataa atgggttatc accgttcaac cttttctcat
     6661 aataggaatt cccttctctt ggccctccta atattgacgt ttcctcctgc ataatggtaa
     6721 tcgggttcac agattctaga tattcgttaa tatttgaagg ttgcaaactt atatcctcgg
     6781 cccttattga ttcctttttt tgaacatttg agattgcctt cttcaaatcg tcctcgttta
     6841 tacttgctga atctttcatt tctgaggtag catcatagct tgacgaatcg aaaacttcag
     6901 gaaggaattc atttctaact gttgtagttg tggtagctga ttgttcacat ttcaacgatg
     6961 aatgatcctg tttctcctcg gccaatacgt tcttggtctt ttcctttgta tcgagggtct
     7021 tgttactctg actgtccttc ttattgttcc tataatcttt agttgctggt ctgatcggtt
     7081 tgttgggacc tcttcttttc ctttctgtca aactcgtatc cagccggaaa cctagcagat
     7141 atataagtta ttcattaata ctaatttcta tcgcgctgcc taacaggttg gctggtggta
     7201 agtattgtaa taccatattt tttgaaatga cgattgagac tgtaacagaa gggttaggtc
     7261 caaggtattt tagatatcta tctaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagatatc
     7561 tatctaaaat atcaaaaaat aaccccctga ctatggcaaa ttacaccaaa tatacgaata
     7621 gagagtaacc catcacatcg ttacaaagag gacactacta gctatctacg gaattattac
     7681 tttattgctc ctactgttgg ttgcgacagc agtttctatc gatttgtaaa catctgtata
     7741 actattattt ggctttatcc tgtctatttt tccgatatga gaagcagaca tagctactta
     7801 agaacgactt gagaagcaga cattcaaatg taatcggtta aaatgacttc gaatccaaat
     7861 tttttgcctc taaaagtacc acacgtctgc caacaagtgt tccactatgt ctatgaattc
     7921 ttaaccgatt ttatctggtt catccataag tcttcgttca tcaactttgc ttttggtttg
     7981 aacctacctg ttctattttc ttacactatg tagctagact tgatctttta cttccgttcc
     8041 tagcctctta accatttaag aatcattttc ttctgtggct cggttttttg tcgaaaaata
     8101 atcaacaaag tctttggaaa tgttggctac ctcagcaaga tgagctcgat tggtttcgat
     8161 cttctcttga ccgatccaga tgatatttcc atcaagatcg atctctaacc ttcttgatct
     8221 ctcaagatat ctgagaatcg tgtttatctt cacaaggttc aacgttggtt ggagtaactt
     8281 ttgcaaattt ctgacttttg ggtatcccga taccttcttg atactgttta ggaaaaagtt
     8341 tatgtcatct tcagtaatct cgtttatagg tcttactttc ttcttttttg taaatttgtt
     8401 ggatttgata aattattatt atatcaatgc catttaaaaa attatgcaac aagaaaaaca
     8461 aagaatacac tcagtattta cttagagtgg ctggcaaagt aggaagacgg acatttaatc
     8521 agtccgatct atttgcgata ctaatatggt ctcttctatc ttaacaatgc tgcacgcaat
     8581 atcattcctc catcaaacgg aaatgcacct tgcaatcgct tctatatggc tcttcaatta
     8641 catatatcaa taaattacac tacaaagtta cgttacaaac agacgaagag aaatgttacg
     8701 aggtagtaaa ataatatggc cacaacctcg gtgggatcaa gaacgcgaat tgagctgtcc
     8761 ggacctcgaa tacaaactga tagtatgcaa acacatctat ccggaaagaa cttcggcgca
     8821 gaattctatc ccaagagcta gctaaatagg atcattatgt ctggatcatg gtaaaatatg
     8881 cggaatataa taagtgcaac taatatcaat tttgtagaga taatacttgg caaattatat
     8941 gccctttggc ttagagaatt caaagtattt ctaagagagc gtagtaggtt agttgcatct
     9001 atcttcaccc cactgctctg gctatttgtt attggttctg gttttggttc cgccgtacat
     9061 ccttcaactg gagtagacta tcaaatgttc atatttcctg gcataatcag catgtctata
     9121 atattttcat ctgtattttt cgggtcatat ataatctggg acagaaagtt tgattttctt
     9181 aaaagtgtca tggtagctcc ggtcggcaga aatactattt ttgtgggaaa gacattaggc
     9241 ggcatgacta cttctctagt agaagtagta atacttttgg ctgtaggcgc agctatcgga
     9301 attcacttta ctgcactttc tttagtccag actattgcaa ttgtactgct tctttcattt
     9361 tcactaacct cattaggtct tattattggt agctatatag aaagcctaga gggttttcag
     9421 ttaatagtga gctttgtcgt atttccactt ttctttttaa gtggagctct ttttccatta
     9481 agaaatctcc ctggatggct ctctcttttg actaaaatag accctgctac ttacgctgtg
     9541 gatgccctta gaaatgccat attaggaatt caaaattatt cgttcacaac agatattggt
     9601 atcttggtcg cctttacgat agggcttgga gtgtttggag gatattcctt tggaagaatg
     9661 aaggccgttt aagtaatgtg ccaacgacaa aagttctatt tatggatatc ctatgtaata
     9721 agactacgta ttcaattatg tataatgtaa tgccaaaggt gaaggatgat ttgtatcaat
     9781 cactcggctg atcgggatca tataatacgt ccataagttc gggtcatagc aagtgaacaa
     9841 tgactttgta gaagcatttg acgcggtggc gaaggaagtg aaggagatac gatatcagga
     9901 tgttcagcac gtatgttaat tagcagctta ccgaaaattg gctgaacgtg ttattctatc
     9961 gaagagggta tactcgctct tgccaacact ccaaacaata atcaccattt tcctgaaata
    10021 tctctgctgc caaattttta cactgattac ataaagtagt attgtcattc ctgtttgcaa
    10081 tcataatatt ctgtcaacta actgttgtac ttacacacat aggtaacaaa ttcacttaca
    10141 gactt
//
LOCUS       DAOR01000167           11767 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17558, whole genome shotgun sequence.
ACCESSION   DAOR01000167 DAOR01000000
VERSION     DAOR01000167.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17558"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3590 a         2318 c         2530 g         3329 t
ORIGIN      
        1 aaccatgtct ggaagtacaa caactaatca acatttttaa gaatctgtaa gaagctgagt
       61 acgatttgta caaagatgtc tcaataatag caggaccatc atctctcgat ctggctaacg
      121 caatcgcaaa gcatctagat gctgaagtag tgccggttga cctcaggata tttccggacg
      181 gcgagagcaa aattaacatg agaagggttg acaaagatta ttgcatagta gtacagtcga
      241 cctatcctcc aacggacagg catcttttac aagcactaat gatgataaaa aggtgttcag
      301 aggatggagc cgcaaacgtg tgtgcagtta tgccatatat ggcatattca cgccaagacc
      361 gagcgttttt ggatggagaa gtggtaagca tggctttagt agccaaattg atcgaaacgg
      421 taggaaccaa gcgattaatc acggtggaca tacatagtcc ggcttcgtta tcgtatttta
      481 cgattgatac gcaaaacatc agcgctgttg gtcttttggc agactatgct gctgtcaaaa
      541 tcaaaccaaa tgcgccaatt gtagtatcgc cagacatggg aggtgctaaa agggcagtcg
      601 aattcgctag gatgctggga acagatacgg ttgccttaaa gaaaagtcga gacaaggata
      661 ctgcggaagt cattattgaa gaaaaagaat tgaattctaa cgtggttggt agggatctga
      721 ttcttttaga cgacatgata agcacgggcg aaagcattgt agaagcttgc agatttctaa
      781 ggaggtacaa acccaacaaa atttatgcaa tttgtactca tgcgttgttg atcggagatg
      841 cgacaacaag aatcaaagcc gctggggtag aagagatcat ttcgactaat tctgttcccg
      901 ggattaatgc gaaagtcgac ctcgcccctc ttataactac taaacttaag gctattattg
      961 acacgaagaa gcacttacag ttataatgta tcaaaatcta cgcagcgtcg acaacaataa
     1021 cgagtgatta tgagaagaaa tgcaggagtc tactattcaa cgtaaaaagt atatactatt
     1081 cgcgctgaga ataacaaggg gcatttacga ggacagtatg gttgatatga agttgatttt
     1141 tcttggaacc tctgctgcag ctccaacagc agagcgcggt ttatcttcag tcgtgataaa
     1201 aagaggaaat gaattattgt tatttgatgc cggtgaaggc atgcagcgaa atttcataag
     1261 ggctggttta ggaatgaata aacgaatgaa aatttttata acgcatatgc acactgatca
     1321 ttgtgtcggc ttgcttgggc tcttacaaac aatgtctttg caaggaagag taatggcagt
     1381 cgatatatat gggcaaggaa aattagccga atttgtcacg gaaaacatgc gaatagtaaa
     1441 tttcgggcta ctatttaata tcaatatgca tataattgat tgcgagggtc ttgtagtaaa
     1501 agagaaagat taccaaataa cttgttgcga atcaaagcat ttagtccctt cattttccta
     1561 ctgtctcact gaatttgata gaccaggaat ttttaacatt agtgaggcta aacggttcca
     1621 tatcccagag agtgatcttt atagcaaatt gcagcacggt caggacatta tctatgaagg
     1681 ggaattgata aagtccagcc aaatgactgg accggcgagg gctggcagaa aaataggaat
     1741 atctggtgac acaagaccca cccttaaatt gcgagatttc ttcaagaatt gtgacctgct
     1801 catcttcgaa tcgacgtacg gtcatgataa ataccaaaag gcggtcaaga attttcattc
     1861 tacagcgaca gaagcagcta tgctggcacg ggaatcagag gtaaaaaaat tgttccttac
     1921 tcacttcagc acgagatatg aagaaacatc tggattaatt aatgaagcaa gagctatcca
     1981 tgaaaacgtt gaagctgcag aagatctgaa ggtagtcgac attccatacg gtgagtctga
     2041 gtgatcatac ctgggtcata atggctggct gcagtcatgt aatttgaatt tttgaatgac
     2101 aatcgatggt ttgagaaagc cagcttctac atttttagga tctatgcagt tgcttatttg
     2161 atgagacgca ttcacatctt agttccccaa ggtcattgca ttttacttga ttctgttaaa
     2221 ggcataattc cgttgactcc gataacgata gtacttgtgt ttaatagtat aactctgtaa
     2281 ccagtagggt taaaaagtgc tatatccgtg atcgttgtgg agttactcgc cttggccagc
     2341 aatccagacg gtgagagatt ggcataatac atgcacatat acccgggctt cgacaattct
     2401 ttgactatcc ctaattttgc cggcttaaga tttgtgagct ggccgactcg tatttccagc
     2461 ggactttgaa gattcgtatc acagggaagc ttcgcagtca aatacccatt cattatctta
     2521 tacggcgtcg agtcgtaaat atgtataaag tcttttggag ggagaatctg atgtgatagc
     2581 agaactgtat ccgagtccct aacaaacata attccagaat tagtacgggc atttgtatta
     2641 ttttttgccg tgctgttacg gatttgatca attgcggagg ccatcaatga tttcggactc
     2701 atgaaagagc ccattacgga ttcaatagtc tgattagaga ccccgccttt cgctgtaaag
     2761 tgtgtggcat ttgtcatgtc ctttattcct tgttgtgcta ttgccattgg cggaaattcg
     2821 cctatcggag agactgactt tgacaatact aataatacgc tgatgatcgt aaagccgaaa
     2881 actccgaaat agttcgctac ttggtatccg tccatgattg ggagtaattt ctttgccgag
     2941 taaaaaagaa taagtgttat attttattga tagatatctt acttactttc agtatcaaat
     3001 acgcattgga cgattcatat cgaatcgaga aatacttctt agaaaaccct gggaaaggat
     3061 ctgatgagct ctactcctgt acaaaaggtt tgtcaggata cgatctgatc gttatcggta
     3121 atgcagtcaa attttaaaat cggagtttca atcgtttctt ccttgcgtgt aataaaatta
     3181 aaaataaggg tagtagctaa tgcgtattta gatgtacgca ggctcatacg aaatgccaaa
     3241 atgcaccgcg tgcggtaaag aagtgcgtcc aggcgagcta tcagaaaatt ttatttgtcc
     3301 taagtgtaaa actgaaaaaa tttggcgttg cgaacaatgt aaaggaaagt taaataaata
     3361 tcaatgtgtt ggatgtggtt atagaggtca gtagtgattc ttcagctgat gagattgata
     3421 aacctgctaa tagtttcctt ttgatcggat tgcagccgat caaaagactc tataagttcc
     3481 ttttctagtc cgatctgggc tgaaatcacc gaacgcagtt tactaatatc catcggagta
     3541 ataccttcga ttaaacctag ggtaaatgcg cggacatatt cattgataat tttgcatgcg
     3601 aaagatggaa agaatctgat tagcaataaa atcccttccc gcattatttc gtagtcaggg
     3661 gtaccactcg gaagagatct aatatatgac tctaacattt tcgttctgct aggacctacc
     3721 tcttctattg aagaggcaac aagaagccct gtttcactca attcatagaa agggacaccc
     3781 tccatctgca aagctttggg accgcgccgc aacggcaatc tacccccttc tatgacaaca
     3841 cctgatggga ttaacacttc gtcaagatct ctgaaaattc cagagtatat gttttgccat
     3901 aaaatgccgt gtctatcggc aataatacgt gctatagaag tccttgttcg tagctccggg
     3961 ctccgctctg tcgcaagatg ggctattatt cctcgctgcc ttcttgcctc cccagtaatc
     4021 ttgttgtttc gagtcttgaa cgtgtcaaat attgcgatat tacatttctg cgatttcaac
     4081 gagcgtcggg tcctcctata aacgacactt atgataatat tttaatctgt tggtattggg
     4141 atataattct gatgaggaaa taacatgtaa gaaatcccat acattcaatt tgcaaataac
     4201 atatcccaac actataatgc taattaaaat tgagggttga gcgctctcga caggaaggct
     4261 tataacgcta cacgcccacc accatatcaa ggaaaatttg caaatcaaag acgggctaac
     4321 gtttgacgac gttctcctag tcccaaaaag atctgccgtc atatcaagat ctcagacaga
     4381 tcttagaact agactttcta ctaatatttc gctgaatatc ccattaatca gcgccaatat
     4441 ggacaccatt acagaagctg ggatggccat tgcactcgcg agggaaggtg gtatcgggat
     4501 tgttcacagg ttcatgacaa tcgaagatca agtagatgaa attctcaaag tgaagcgttc
     4561 cgaatcagtc gttatcgagc aaccttatac tgttaaggag actacgactg taaaggatgc
     4621 aaaaaaaatg atgacagatt atggcgtatc aggacttctc gttgaagaag acggaaaaag
     4681 acttgccggc atcattacgc aacgagatat cacattggag aagaatggtg gcagtaacgt
     4741 tgcagacttg atgagcaagg atgtgattac agcgaaggag gggaccacta tagaacaagc
     4801 caaggaaatc ctgcacaatc acagaatcga aaagctgcca gttgttgatg cgacacggcg
     4861 gatcgtcggt cttataacga gcaaagatat attgaaaatg gagcaatatc catacgcttc
     4921 taaagataaa aagggtagat tactcgttgg cgctgcagta ggagtaaagg gtgattattt
     4981 agaacgcacc gaatcgctcc tggaagctgg ctctgatgcg atagtcgttg atattgcaca
     5041 tggtcacagc gacaatgcga ttaatgcagt gcatatgatt aagaaggcat ttccatattg
     5101 cgagttaata gctggaaata ttgcgacagg tgaaggaact agagacttga tcaaagctgg
     5161 tgttgatgca gtgaaagttg gagtaggatc tggatccatc tgcattacga gggtcattac
     5221 aggctctggc gttccccagc taacggcagt aatggatagt attaaggttg caaaagagta
     5281 taacattcca ataatctccg atgggggaat acgaacctcc ggcgattcaa cgaaggcact
     5341 cgcagcaggg gcatctacag tcatggtggg aagcttattt ggtgggaccg atgagagtcc
     5401 aggcaagaca atcgtcaaga acggaaaaaa atataaaatg tatagaggaa tggcttcatt
     5461 ttatgcgtca ctaggcagaa aatatagaga agaagggggc caaacagcag acgcagatga
     5521 tctaaatgat tatgtgccag aaggcgttga agcgatggtt ccatacaagg ggagcgtagt
     5581 tgagattatt aggcaagctg taggagggat aaaatccggg ctaagttatt gcggcgcaaa
     5641 aacgatctct gaaatgcaaa gtaacgctga gtttatcaaa attacaccag ctggttacac
     5701 cgagagccat cctcatgatg taaatgtcat ttaggaaatc ctatctatta atgaactttt
     5761 gtacatcttc tcatttatct aacagatgct ctcctcacaa gctcttgctc gagattatac
     5821 caatataatg ggtagctagg gcgcagaagc ataaagttct agcgaaaaca tcgtgtctaa
     5881 ttcacgaaga catcgtgcaa tatcccagcc agtcttataa tgctatctac tataacacat
     5941 ctctgaaatt gacgggacgt cttttatggc agagtaatca tctttataga cggaataagt
     6001 tagtcttcga tatgcaaatc tatcgtgttc gaaacaattg agatttcttc gggttcggta
     6061 aagtaatgcc tcccccaaga aactttgata tcggcgtagg ttgtgtggtt cccatgatta
     6121 agattattcg tctctatctt gaatatttta ttgacatcaa ataataatga ttttgcctcc
     6181 tcgattgtga cgggataaaa cggagtatca tctctaataa tcgataccca tattcgaaat
     6241 gggatctttg ggctacgtgt ccagaacatc ggtgcctttc ttgtaatctt ggttaggaag
     6301 atcttcctac tttttacttt cagcgctacg gatatagaca tcctgaatac gttatcatgc
     6361 ttatcgtacg ctgtctccca catcttcgga ttaaaaactt ctcggatatt tccgtcaatc
     6421 ttaaaactca aatccaaagt gaccggctcg ccttttttcg ttttattttc atggtgagtt
     6481 ttgttcagga tgacatttgt aatgctactc tgtctgtctc tcaaaatgat gccttcatgg
     6541 tatcatttat atcctcaata agtagttgtc tgactgatgc ttgcagaaat tgtcgatata
     6601 aaagatcgtg aaatcgaatt aaaactccag gacgaagaca tttcagtgat gtacatcgta
     6661 cagcatgaac ttcttaaaga gaagaatgtt gaatttgctg gtgtagttct gaagcatcct
     6721 ctaatcaagg attacctcat tagagtcgta accaagaaga atgtgtctat ggaagcgttt
     6781 caagatgcct gcatgtctgc ttctgagaac attaaattgc ttaatagctt aataaaggat
     6841 gccttaaaga aatattcagc ttaggtgtaa cctcgtgaag ttttgtccta aatgtgaaag
     6901 acgcatgaaa ccgaagattg aagaaggcat cctctcgtgt ccaaaatgtg gtcttattgt
     6961 ggagagagaa gacgaaaaaa cgcaagtaaa agggaattcg atacggaatg cagatagttc
     7021 ctcccaaagc tctctcaaaa tcatggaggc cgagaaggga ttggatgcat tgccggcaac
     7081 aacgattgat tgtccaagat gtggaaatga tatggcattt tggtggatgc ttcagacaag
     7141 gtctgctgac gaacccacca ctcaatttta tcgctgtaca aaatgcagcc atacttggag
     7201 aaattatggt taattgtatc ttttgtcatt tttgtgcttg tatgtttttg ccctctcaaa
     7261 acgctcgtcg tccgcgtggc aggttcccgc atgacttgat tgcctgtttt tagcacagca
     7321 atgtttaaaa tccgaagatg agagccacaa gatagaattg gtttggtatt cgtagctaag
     7381 acaagatcgc cagacgaatg gaaggttatt gtatctgcta tctctacttt agttgacgaa
     7441 gcaacctttg aagctacagt ggagggaatt tcattcagag ggatggaccc gtctcacgtg
     7501 gcccttatag atattcattg gcctaattcc acctttgagg gatacgaatg tgatgattca
     7561 gtaaagtttg gtgtgagggt cgacgaacta tcaaaattga tcaagcgagc tgataagagg
     7621 gacgcaatag aggtaagtat gggagaagac agcatgttgc atattaaaat gtataatggc
     7681 tataaaagag aatataaaat aaggctcatc gagagttccg cgagttcgac tccattacca
     7741 aagttgaatt tcaattccaa gctggtgtta aatgcggttg catttgataa agttctatcg
     7801 gacgtgcagg tggtgtctga atacatttcg atagaatcgg aatccaagcg ggtagaattc
     7861 tcaggtaagg gagattcagg tgaagcggac ataattttgg aatccgaaag cgcgggttta
     7921 gaagagctag aggtgaaaga aaatagcaaa gcaacataca gcctcgatta tttgacgaaa
     7981 atcacaaaag ccgtaagtgg agcaggagga tcaattgcag cggaatattc aagcaaaatg
     8041 ccactgaggt tggaatttag aatggctaac gtagggagaa ttcacttcta tcttgctcca
     8101 agagtgcaag attaagtttg accacaatcg ttgcaaaggt aatttatctc tgcatgccac
     8161 tccctgatcc ttcagcatta ggacggaacc ggccttatca atcaggtggt caccaacaga
     8221 tctgtggttg tccgcgaaac tctgcagtag gcgtcaggag aataataact atgaaacaat
     8281 aaaaagatag atacgggtac ttcagtatac aatttttcgt ccagttttgt ccctgtaata
     8341 catgccgaat tcattcattt tagataactg cttttcacag aatacaggac catctttaca
     8401 aacgagttta tcttcaatac aacaactagc acaaatccct attccacatt tcatatgtcg
     8461 ttcaagactg gcttgaatag gtagggaata cgcagaagca atagctaaaa cctttttcat
     8521 catcaattcc ggaccacagg tatatacact atcgaacgtt tcctctttaa gaacttgagt
     8581 catagcatca gtcgcagttc ctttaaatcc ataatcaccg ttttccgtac tgactattat
     8641 cttgtgttta gtatcactca gaacggcatc ggctgtattt tcaaaaaaaa cttctctttt
     8701 tgaatttgcc ccaattatca gtctgcaaca aattctcatt tcgttcagtt ttgcggctag
     8761 tctaattaaa ggtacaagtc ctgttcctcc accaaccaat agcaccgttc ttgcattcgg
     8821 ggcaatcttg aaattgttgc cataaggacc acgaatccca agcatctcac caattcgttt
     8881 attgaacagc gccgtcgacc caattccctt tctgcgtatt gtgatcgcgg cgctatctac
     8941 ctcgtcagca accatgacgc tcatcggtag ctcttcgacc cccggaatcc agaccataag
     9001 gaactggccg ggttcagcct tggaagagac tctatcctta aagaggattg tcttgacact
     9061 atctgtttca ttaagcaccc gttcaataaa cacgcttctt ataatgttaa tatttatggg
     9121 caagtcctac taactccgtg aggttcttca ttcccttcct ttctaagtat cttttaagac
     9181 ccaaacagat tttctggaat gccccaaatc cttcgaaacc aatcacacta ccaagctgta
     9241 tcgctgatgc ccctgcgagc atgaactcga cagcatcttc ccagctgaat attccaccac
     9301 aaccaatcac cggtaccgcg acatttttag aaatttcata aacacatcta atggcaatgg
     9361 gtttcactga tttgcccgac aggccgccaa ttttattact caggaccggg attccacttt
     9421 caatgttgat agtcatggca cgaattgtat taattgctgt aatcgcatta gctcctgcat
     9481 cgtttgcagc ccttgcaata tcgagtaggt ctacgtttcc aatgccaact ttaacgacaa
     9541 ccggcttgag tgtatgagac tttacagtct ttacaacctt ggtcaccata tctggatcat
     9601 ctcctacctc catgcccatt ttggctacat gaggacatga tagattaatt tcgtatccaa
     9661 gaatatttag gtcatcgaat ttgtttatta tttttggaaa atcgtcatga gatgaaccaa
     9721 ccaaactgac tattatcgga acatatgggt tggaggcgat ttcactcgaa aatgcatctg
     9781 ctccagggtt tgcaagaccc acagcattca gataactccc gccttccaaa catactgcag
     9841 taggatttct ataaccctct atgggctcgc agctaattga tttgctcact atcgctccaa
     9901 catctgcgtt gtaaagcctg ttgaaaatag cttgagaaat acctaaaaaa ccagaagcaa
     9961 gcatagtggg attacgcagc tccatacctg cgatgtttat ggaaagcgtt ctatcatgtt
    10021 ctctctgtct ctttcctctc aataatttac ccatctcgag catcttagtt gcacgactag
    10081 ttcatcgtca gtactcacca tctagaacga aatctcttag ttggcatgta caagaaaaag
    10141 cttccgcata atacacaagt acttggacaa tgagtcaata atcttactat ataggattca
    10201 tatcctggca caacttggta ccataggtct cctcaaggat tataaaaatc ccgtgcatac
    10261 taaatttgag ctaatttggg acgtccagta attgtcaggt atactaattt tatcagccta
    10321 cgcagacaaa gccaaattat tactaaagat catccttagc gatgtcttat tgcggaatag
    10381 gaataggcac tggtacaggg atcttgggcg aatcggaaga aggatagatt attattaaat
    10441 actttgacag gctgtattag ctatcgcatg gtttatttca cagacattcc tggagtcgtc
    10501 gtgacaggtg ccagatgaat cctgatgtat aatacaagtg aaccacagct agatgaaggc
    10561 tttgttcgaa acattaaaga aggactagga gccagggacc agaagcgggt taatccaaag
    10621 tatctttatg atagtgcggg atcgcagctc tttgaagcaa tttgtcgtca accagagtat
    10681 tacccgtggc ggacggaggc agcgatatta agacaacatt ctacagaaat ggtctggatg
    10741 catggcaaga aaaagataag tattattgaa ctcggaagcg gcagttccag caaaacgaga
    10801 atccttttgc agaaggttct cttgagacag cagagtctat attattttcc tgtggacgtt
    10861 tcccaggcga tgttgcgtga aacaatatat aacctttctg ccgacttttc tagtcttcga
    10921 acgataggaa tttcttcaga ttatattgat ggcattgata aagcaaatag tttgattgcc
    10981 gccgatcgcc aaatccctga caaaaaattg ataattttcc taggatctag catcgggaat
    11041 tttgaaccaa aagaatctgt agctttcctc agaaatttaa gaagtaaaat ggagaagagt
    11101 gatgatctct tacttggtat cgatcttcat aagaacaaaa acgttttgga ggcagcttat
    11161 aatgacaagg agggtataac agcgaggttt aacctaaatc tattgactcg cataaataga
    11221 gaattaagag gagagtttaa tctcgatagc ttcgttcacc atgcttttta caattcttgc
    11281 tacaaaaggg tcgaaatgca tctagtatcg accaaagatc agcaggtata tgtaggcagc
    11341 ttgggtcaga cctttagctt tgataaaaat gagacgatcc acactgaaaa ttcgtacaag
    11401 tattctgaag aggaaataaa acaaatggca gaggccagcc aatttatact aaagagaaat
    11461 tttgtcgatg aaaaaaagtg gttcaatgtc gcactctttt ctcctgcttg atatgcatat
    11521 gccttttttg atttcaacct aatccatctt gttcacgctt tccctacaac catatgatcg
    11581 atggccctga ttctaatctc agaagtcctc ttccacacat ccctatagtg ctatgatcta
    11641 tgtattagca catattcttt ggacaagaac aggctttggg gggtaacgat aagaggtttt
    11701 gtagccctag gatatctctg atgtggtggg gatatctaca tacttctctc ggacggggga
    11761 aatgaga
//
LOCUS       DAOR01000168            8911 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_59393, whole genome shotgun sequence.
ACCESSION   DAOR01000168 DAOR01000000
VERSION     DAOR01000168.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_59393"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2826..2931
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2725 a         1742 c         1825 g         2513 t
ORIGIN      
        1 gactaatacc agtatacatc gtttccacaa aaaatatatg gtttgatgtg gctgattaca
       61 acgttgagac caaaggcaat actggcccta tacaaaaggg tgtttctcgc ccaaaaccaa
      121 atcctaagtg gggtagggaa gaggaatctg aaatgttcgc tcaaaatgtt aaacatttca
      181 gacaaaaaaa gctttccgat gacgattttc gtcgttttag gttgcagcac ggtgcatatg
      241 ggtccaggct gcagatgaat tatagcatga tccggatcaa agtgcctagc ggtgaggtca
      301 acccagaaca gcttgaaaag attgcaagtt tatccgaggc attctcgatt ggctcggctc
      361 atgtttctac tcgccagaat attcaactac attgggtaca actggaagat gttagtgagg
      421 taatgacggg acttgtagag gcaggtctga caacaagaga agcttgtggc aatacagtca
      481 gaaatgtaat gtgcagtcac ttcgcaggtg tctgtccaga cgaaaaattt gacgccactc
      541 actatgccaa agcaattgca cgtttcttca taagaaatcc aatgtgccag aatcttccac
      601 gcaagttcaa gattaatttt gcgtgctgcg acaaccatgg gttagcacga attgcagata
      661 ttggtttgat accagaaatc aaggatgaga agcggggatt caaaatctac ctcggtggag
      721 gtttgggtgc tgcgtcattt attggccacc tggtagagga ctttacacca gagagcaaac
      781 tccttcctac atgcatggca actatcagac tttttgatag acttggcaac cgtgaaaaca
      841 tggcaagaaa caggatgcgt tacctcgttc acgaaacagg atgggataaa ttccaaaagt
      901 tggttctcaa ggaacgctcg atagtcgaga tgactaccgc gagttatacg tccaatcttt
      961 ttgacgtcaa agctgaagag gaaacgaggc cccttcccga ggcagtaaat gccaagatgg
     1021 ttaaactacc tatgctaaat gaacgagtaa ctctggaaag tcctgcatat gaaagatggt
     1081 tacatagtaa cgtggttccc caaaagcagg acggatattt cacagcattt ataaccttag
     1141 cagcaggcga tattacagcc aaccaactgc gaatattagc aggagcgatc cgtgaattct
     1201 ctgcagaagg aaccgctagg aacacgccgc agcagaacct tgcaatccgc tatatacagg
     1261 ctgttgattt gccagacttt tatagtagac taggttcgat tggcttggca aatccaggag
     1321 ctttaacaat cgcgtctgca gttggctgtt ctgggactac ctcttgtaat ttggcaatca
     1381 caaattccca cagactggca aaagaagtcg agtacaagtt tttggaatta ggacttgata
     1441 ctgatgatag cctgagggat tctaccataa agatcagcgg ctgtccaaac tcttgtgggc
     1501 aacacgagat cgcaacaatc ggattcttcg gtggtgcatc aagaatcgga cccactatgg
     1561 ctcctactta tacaatgcta tttggcggaa gcgcaggaga acaaagtgag ttgggtaagg
     1621 caatcatgcg agtgccggca aagcgcgtta tagatacaat tcgcaagata atcgaaatat
     1681 acaaacaaga acggtctgac tccgaaacat tgaatcagtg gatcaataaa attgtcaggg
     1741 gcgaaggaac aggaagcgta aggaatattg aaaatatgaa ggcatcactc accccagtta
     1801 tgcaattact tccagtagaa caagaccagg aagtgtatag tgattatggt agtgatgcaa
     1861 gattctcagc gaagactgcc aaaggagaat gtgcagcata aattattatg acaatagaaa
     1921 acgcatccgg atcaaaaacc gatacgaagg aaggcatcgt tgaaactaac gagaattaca
     1981 attttcaact tccgataatc tgcgatgtag acacattacg catgttgatc agaaaagacg
     2041 gagtgcgtgt ggtggatgtc cgtaaaacag aagaatacca acaagaacac atacctagtg
     2101 cagtatcact cccgcttgca cagttactag agaaagacat acctactgcg atcctggatc
     2161 ttttacaaaa tttaggaata tcagataaaa tgccagttgt gatctatgat aatacgtttg
     2221 gcgcacttgc ggctagagtc gcctggtctt ttcaatacat agggcatcca aatactgcat
     2281 tgctcgaaat gacattcagt caatggaaaa acttaggact ggtagtagag acgaaaatta
     2341 attcttttcc aatgaaaaaa cattcactaa ctataaatca cagcattcat gcagatgcat
     2401 cttatgtcga ggctgcaaag gaacagccaa acaagatact tatagattca agggagcggc
     2461 tgaactttct gacagaacat attcctaaag ccaaaaacat tccatataca atgcttgggt
     2521 ccaatgggag cattttgcgg agcccacaag agataagacg gtatattgaa aatagaggaa
     2581 tcccggttga tgctgagatt attacatatt gtggcagtgt tggaacatta tctggcctaa
     2641 catattatgc gcttaagtcc gccgggatag aaaatgtaaa gctttattcg aagtcgttca
     2701 gagagtggaa gtctctggga aaaccgaagg aagaatttaa ggacgcaaac tattgggacc
     2761 tctcagcaga ttagaaagac ttggttccgc aaccaccagc acctaaccgt aacccctacc
     2821 aatgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgtaaccc
     2941 ctaccaatgt agtttatcct ctgaaggctt ttcagaaggt aacgttggtc acatccccga
     3001 cttctagtgg cttattaacc aattgatttt ggtgaggagc aaagcgcgag tattctctat
     3061 ttgcgaacat taaaccaatg tttgttcata atgactatct aaatatcgat gggtatattt
     3121 atgtaataat tatggagcgc cattattata caattgttgc ttcgaaggag gatgtagcaa
     3181 gcatgacaat ggcaaattat ttgataaaca accgagcttt ttcacgagaa acaaatacaa
     3241 atttccttca gtcaaagcac catcaaagta tcaaactcca tgtttccaat agaagcttgg
     3301 tctatcttga agatctagat gaaaactatc ctaattcgat agcatttatt tttctttcag
     3361 agcacaggtc acaaaaaaga atccccactc taacttgtca ttgtacaggc aattatggtg
     3421 gtaatccgta tggcggaaat ccacgagaac tgggtatagc ctatccatat ttgcaaaagc
     3481 agtatctaaa ggctattgtg accctgaggg cgttaattcc aggctacgac gtcgtcatcg
     3541 aatcaagcca tcatggacca acctcattaa aaaaaccagt ccttttcata gagattggat
     3601 caacggaaat tcaatggacc gatagaaata gtagttcggt tgtatgtgag tctatattaa
     3661 aagtaattga caacggtcta ggatcttgtg aaaaggtagc aatcggtttg ggtggaacac
     3721 attatcctat gaaatttaat aaattgctgt tggagtcaga atatggtctt gctgcagttg
     3781 ccgccaaaca caacctggag tcgattgata aagatatgtt agatcaaatg attatcaaga
     3841 gcatagaaaa agttactcat gtaatagttg actggaaggg acttggaagc gagaaaaaca
     3901 gaataatgga gctaatagga agcacaggac ttgatattct gagagtttga aatcaggaaa
     3961 ataaattgac atataaaaaa atccaggtta tggattttaa caatagtatt gcctcatgaa
     4021 cgaaactgaa aacataggcc caacatactc ggggaaatga aagtactatt attgcagcga
     4081 gactatgaga ggtgggagaa tatggctaca aaggaaccga aaaatagggt gaatataata
     4141 acagtttttt tagagcacga caaaaaaatt ctaatcctca aacggagcca gaaagttaaa
     4201 actatgaaga caaaatgggg tggcataagt ggatatatcg agcaggagga gccggtaaga
     4261 cgagctctaa aagaaatcgc agaggaaact ggtttgaaga acgaaagtgt aacgttcatt
     4321 cgtattggcg agcctttaga ggctgttgaa tctgatgatc caaagataac atgggttgtt
     4381 catccattcc ttttcaggtc aaatacggat gaaatcagaa ttgattggga acatgacgaa
     4441 tacagatgga taaatccaaa agaaataaaa aattatgaaa ccgtaccaag attaggggat
     4501 gcattcgaga gcttgctagt ctaaagtaca accaccaatt atggattttt ctgtttggcg
     4561 ctactgatgt tatcgactgt gcgataggca aataaaagca gccaagtttt tgcctgataa
     4621 aatattatta gtggggattt tcgatactaa tattcttgag atattctcaa aataatacca
     4681 tacttatcac aactagataa ggcttttaaa acatgatatc gcattgtttg atatttactt
     4741 gaactttacg cctccatgac ttgagtattt tacagacaag aaatatttcc ttcgttactt
     4801 attattggtt cagcttcttg ctggattgtc attcttttat tttatccaat cctgtagcca
     4861 tcacgtcagc cacacggttt agattgttgg ttctgcattc taaaataaaa tctttcatcc
     4921 atggaatatc ttaagcctca aagacgacct tatgttcttc ttcttgtttt ctgtgccgac
     4981 gtcacgtcca ttttcaggct gattttctgt tactggcaag aatattgtag ccaataatag
     5041 tgatcctaga taattattac ttaaaggtgt gtttgcgctg gtttcatccc catctggagc
     5101 tgaaacgaaa tcaatagctc agaactcctt aagctaactt tattaccata gaaaaagtac
     5161 gtagtgcggg cgtcgggatt agccaatgaa atgtatctcg ataagtaaca aaccgaacta
     5221 gtcccagact gggaggagaa atcacaaatc tggcaatatg tattgcaccc taaaggcgca
     5281 atttttttag gcctcataat ggcgttggat ggataggatc ctgtagagat attccctatc
     5341 aagattgggg gagaaaatac tcaccttggc attattgtta ctgttttaag tgtaggaatt
     5401 ccaacctatc taatgttgtt gcgacaacag ccattgctcc acgtattatt gctagtatta
     5461 tgtttgtcat acttatctta ccttgttaac gaagggttat agtctcctat ctaagaaata
     5521 gcagatcggt gaatctgtaa tctctaaaga aagagatgtt ggcttcagcc gctactaaaa
     5581 aatgtcccta ctgtcagact tattgcagag attgcaaaaa tgtttggcac tacagattaa
     5641 gagttaaacg aattggcctt ttggagtaat ggatcgcatg tacagaatca tgattacaat
     5701 gcttttctta aagagctgca acagattgga gaacatatgt caaaacaaaa atagggaagc
     5761 agttttcttg tcattatcag ccttgataat tggggataag aattggaaca gctaacaact
     5821 ccattaataa cggctttgtg taccaacgct ttacttgtga gattttgtgg ctcatgtacc
     5881 tctgtaggcc aaaatggtct cctagttatc aggctgtcac ccattgggaa gtgtatcgcg
     5941 aatgcaatac ggctatatca caaacagaac aaacaaaaat attcgacatt gcttctgggg
     6001 aagcacatgc cacacaaaat ggcatgtcta cgtttagctg gacgttgaga ctaccaaatg
     6061 aaatgtcaca atccgcctag ctcagtattt tcattagatt tgcaattcta tactcctagc
     6121 ggatcttgga ctgttttaca agttccctga ggtgcgccag cgaccttacc tgaccgccat
     6181 taactttctt ataaattccc caataaattc ctcgagttat atcatttcta tccttcaata
     6241 cctttagatg gtgtctcatg gctcttaccc tggctacata gacctgtttc ttgcctgttc
     6301 ttgctgactt tttgcctttc tttgatcctg tgcctgtgcc atgtttcctg cggatggcac
     6361 gcttcgcttc ggcgcgcgct ctagaagttc cccggggctt aaccgtccag atcgttccat
     6421 tctttatcag agtgcgcata gtttctcgcg taatagaatc tgctacatca tcaagtttat
     6481 ccggttcaaa cctgatccta ttcacaccta catcgagtat tcgcgcaatt aattcccttt
     6541 ttttccttat atttactacc aattaatggt tttccacctc ttcctcgctt tgctcaactg
     6601 attttatctt tttgttcttc tcgtttgtct gtgagggcag ttttcctgga ttcagtacct
     6661 taatgcctag gctattagcc ttcgccagaa tctctattct ttttcgtgtt ccgactgtat
     6721 gtgctaatct agctgcatcc ttccttgcat caagtctggt aagatcattt acattgaaaa
     6781 cgatattatc aatatatcct gatggatgaa gacctctacc ggcctttggc cctcgatacc
     6841 caattttaac aaggcgtggt actccggaaa actgctttct catcttgttg tcttttccct
     6901 taggttttct ccagttttca gctaaacggt catatctcca actctcctgt ctgacaaatt
     6961 tgggtctcgc atcagagatt ttttttctag cctgaaggat ctccttgtta atcattatgt
     7021 ttacgaattt aaaagataat ttttgaataa atacgtttct gagtcgacaa cgcctaataa
     7081 ttaaattcta catgcggcac actacataaa gcacgcaaat atatatagat caatggagga
     7141 taatgacgga aataagaaat agggttgtaa attagcatgg atccatcaag ctatcccagg
     7201 ctggcaaaag agaaaggtta atagtttcaa ttgtgtttca tggatcgtga aaaaatacga
     7261 actacctccg ttaccttacg catatgatgt actagaacca catatatcga aggagattat
     7321 gaccctgcac catgataagc accatcttgc ctatgtaaac ggcgccaacg ctgcacttga
     7381 aaaattagaa aatggaagga agaataattt ccaaggtact gatgttaaag ctatagagag
     7441 agacctttca ttccactatg caggtcactt gctacactct atattctggc ctaacatgaa
     7501 atcgggtggt ggcggaaaac ctggcagcgc tttagctgac aaaattaatg cagatttcgg
     7561 cggcagttac gatggattca aggctcaatt tactgaggct gccaagaccg tggaaggtat
     7621 tgggtggggt atacttgcat acgacccgct ttcagatcaa ttattgacat ttggtgcaga
     7681 gaagcacaac ctgctgctcg gtccaggaac tatcccattg ctagtttgcg acgtgtggga
     7741 acatgcgtac tatctccaat acaaaaatga caaggcaagt tatgttaatg cctggtggaa
     7801 tgtggtaaac tgggatgatg tggcgaagcg attcgacaag tcgaagaaat aatggattaa
     7861 tttccatctt cttttgtaat ttataaaacc gcttttaggg atcatgaccg atgaaaacca
     7921 acgatgaatt actgtataat ttgtccttcg tgcaattcga ggtttgataa ttacgataaa
     7981 ttcgttgatc atgtatttga gagtcataag gatcaaccat cattacgaat gaaagcaaag
     8041 attatcaaaa ataatagaaa ctaaagtaga tattcatctc attttcgtaa attacgtaat
     8101 ggatgctgca atagaagcct cagtagctac aaagttgtat agcagatctt ttgcatcgaa
     8161 attaatctat ttattccatt tttaggtttt ttgtaagtcg acgatattaa tcgaacaata
     8221 attttccctt gtagttctgc tcacagccat attgtagttg tatatttttt cagaatattc
     8281 ttttagcacg ggttgaatag aacctaacga tttacttgca taaaagccag gacaatcacc
     8341 ggttaattga aacgtagcat gaatgacagc ttttccttta tcagactcaa tccatgaagc
     8401 gaagggatac agccagcaga cccccggttt ttcgggatga attccacagt aaccgctagc
     8461 atccaggaat ctgcacggaa gcggaaagcc gtcctccgct tccttttcgt cagaattcct
     8521 tttcagcgaa agcattgtaa gcgttgttat aatattgccg aacgattctt gttcctccca
     8581 gcttgagatt cgggtttcag tcctgataaa ctcagttttt gaaagataac ccatcttctt
     8641 ggcaattatt tcgatatcat cttttgtaag tggcaatctt ccttgccgct cgcaacaatt
     8701 gtgacaatct ggccacaaac acttccacaa cacgtagagg ttatcatctg ccagtgtcgg
     8761 aatgtgaaaa acgactccat taacttgtag cgctgtatca accacgtcat ctcttttgcc
     8821 aagatgtaga tcggatattt gtggatctat cctccacctt ttagaaagca gttcaagaga
     8881 atgttttaca gaattatcag cttccatatg t
//
LOCUS       DAOR01000169            2547 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_56028, whole genome shotgun sequence.
ACCESSION   DAOR01000169 DAOR01000000
VERSION     DAOR01000169.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_56028"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    491..742
                     /estimated_length=252
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          673 a          426 c          428 g          768 t
ORIGIN      
        1 gttcttattc ctctatttct agcatcaaga agggaatcct tcactccttt aagttcgaca
       61 gccaaagaag gccttgaata gttgatatcc aaatagaaag tgacaagaac atatctgtaa
      121 tagagatatt tcagctaaag aatgggtgtg gaatatagaa taattaacac atattatatg
      181 cgtgctgcat ctcgtttgtt aagtggtcga tcgtggtcaa tcaactctgg atgtttccag
      241 ctagatttgg catgtctaag ggtgagtacg gatttcatgg atcaggtcat tatactatag
      301 tcaaagagaa atatgtttgt gactggatca atttaatata cagaatacaa tgcagtagaa
      361 aatgattact gcgttgtagg ataaacagaa tatgttatag ggtcgcaaat aataataatt
      421 ttgtgatagc tcatctgaaa acgagacaga gagacgcgag ctatgcaata tgagatattc
      481 ttaatatgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nngtggtaaa tcagcggaaa agggttgaat ctagttggat
      781 accgaagata tcgcattatt gaaatatgac ttgcaaacgt ataacataaa gtgggcgctg
      841 aacttatgaa ggctctatca ttcggaggcc ttgaaaataa agaatgcgca gttatacaaa
      901 ctgctaagat ggttagagag ttagagtggc cccaaattta atgtcctgtt tgcatcatgt
      961 tctccggctc gaatcagttc ctcaatcgat ttagctgaaa aatcagtaaa tttgccgtat
     1021 atagaatccg gctcgtcatg ccgttctata tgccatattt tggttatacg gaatcgtccc
     1081 tctactaggt ctctgtaact tctgttttcg ccgctacgct tcttgctttt ggtttctagg
     1141 tttgaatcta gaatattgtt tatttcagaa tccgatctac cctttttctt tgcaagagct
     1201 atcagtttcc tagtcaaatc gacgtaacct gttacaagat ttgctacctt ttcatcgtac
     1261 tgtgttctgt tatgaaatag tatgtcgtgt tctctatcgt caattgaatc caaatcattt
     1321 ggagtagctt tttctaatgt agcatgcagg tctactacat agatttccag atctggtacc
     1381 tttgtatctt taccctggcc acccgaatag tctagccaaa agtctctatg agattggagt
     1441 agttctctta atggtgtatt gctaaggtag cctccgtccc aaaactgtcg gtcattccct
     1501 gttatcttat cttccatatg cggatgatca aaagtacttt acatacccct tatctttaca
     1561 gcagatctgt ctttcctgtg ccgaaaaaga gataccaaaa acaattgaaa tcttacagaa
     1621 tgacttaaaa aaaataaaag acggttaatt atattctagt gttacatctg gtttgaattt
     1681 tctgcatacg ttgacattat cggatgccct agatacacta ttatgctcaa tagttcaagg
     1741 atagtagttg aagtcttaga atccaactac tcctattaat gtacgttagt taacgggttg
     1801 agtatatact tgacaaagaa aaacgaatag gaaataacaa aaaatgttca tatgctattt
     1861 aatcaagacg attcttcaaa tcctgaattc taattcggag ggcgaattct gatactcctg
     1921 ctgctcgtgc gatatctctc tgagttttat tttctccagt attcatagct gataaaaaaa
     1981 ttacagatgc tgcaagtgcc attggacgtt tcccagcaga tatttctctt ttgagaacat
     2041 cgtgcataat gcccattgct tggcgtttag tcttttcagt caaattcgct ttgtttgcta
     2101 ttttagcaat acagataata ggatcgacca atgggatttt aaggtcaagt ttttttacaa
     2161 gtagcctata atccattgca agggttctta gtttgatatt ggaacttgca gcaatttcat
     2221 ttaatgtcct tggcattccg atttctctac atgcgatata tactgcagca gctagtatag
     2281 aggatattgc tcttccacgc agtagtgctc tttcctcagc ttttctatag atgtacgcag
     2341 ttttctcgac tatgacatct gacaatccaa gcttatcttt taagctatca agttcattga
     2401 aagcctgttt aagacttttt tctacactag tatgcaaacg aatcctagag tcccatgtcc
     2461 ttagtctttt cattctagaa tgcatagctt catccaatct atgtccaata gaatctttat
     2521 tggtccatcc aattactgtg gaaagcc
//
LOCUS       DAOR01000170            2762 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_66537, whole genome shotgun sequence.
ACCESSION   DAOR01000170 DAOR01000000
VERSION     DAOR01000170.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_66537"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2135..2459
                     /estimated_length=325
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          824 a          407 c          461 g          745 t
ORIGIN      
        1 gttattctta ctgattgtgt ttattcacaa aactatttgt gcattaattt tgtgtgacag
       61 tgcaatatac tgcaacaaaa tatcgtttgg aagcaattaa ctaaccaaaa tttcaaatca
      121 gattggggtt gttcaaagaa ataggaaagc ggtatgaata acgtgtttcg caattctaaa
      181 ccgaactata agactttagt ttgtggaact ttttagattt gcttaatgcg agtaaattct
      241 aacaaaccag aaaaatggat gggcaccaga tttcagcgaa tacaagaaat ctcttttatg
      301 tctaccgtga caagtcttgt ttcgcacctt catcatttta tgtactaaac taagaagaac
      361 ctatatcaaa agggatgaaa tggtcaggcg gtagcggttt gttatttttc tgctttcatt
      421 tgtctagaga gtaattggcg tagaaattca ctatacgtat atgcttttgg acatgctagt
      481 gctagttgtc ttttaacttc agtttcaatt cttagtgttt catatgtctt cttctttttt
      541 cttgttcttc tatttggttt tgtcatcaat taatggtgca aatacaccat tcgtaacctt
      601 tatagctatc gttaggtatt ttatatagtt agcaaggagg tgaatattta gcattggcaa
      661 ccaaacaaga atctgttgtg aatcaagaaa ataattacta tgacgaaatc gatgaaatct
      721 taagcagtca atacataacc attggaccag gagagaaaaa agtactgaag ttcgtaccag
      781 aaaggggtat gaccaaagta gaaaaaatgt ataatggtga accgacagag aagtgtcggt
      841 ttattgtaat agaccctaat agcggaagtg atggagaaaa gttctttgac attggtaaga
      901 ggtctgcacg actaatagta ccaaaactca aagaaggaca tagaatgtta agaatggaaa
      961 gagtaggttc aggaaaggaa accctctata ttccagcaga agtagcagct aactcaacaa
     1021 cgaagccaag gtaaaggagt acctttccca attttttgtt attattttcc ttagctccat
     1081 tgtggttgtc tacttcggca tatttcagct gtaccgtcat ttgccatcat ggtacggtta
     1141 cattaaatta cattaaaata ctcttaagaa gtaatttctg agcctctata ggaagtacaa
     1201 gttgcacctt ctgcgtatgc ttattcgcaa gcactatttg tacacaattt ctacgtgaca
     1261 gtgaactaaa ctgcaccaaa aatatctatt ggaaagcaat taactaatta agatttcatt
     1321 atgtttaatc ctaatcatac cattaaatca aacccttcag gaagaagccc tcatgaagag
     1381 acatacacat attaatatca ttagttaata atatatctat gtcaatacag cttcaaaggc
     1441 tatcactaaa agcaaagctt ttcagaggac ttggagattc tacaaggctt tctatccttg
     1501 agctattacg tgatggtgaa aaatctgctt cagaaattgt taaaggtaca ggtcatagtc
     1561 agtctaacgt gtcaaatcat ttatcctgtc tgctagattg cggactagtc cagaacagaa
     1621 gagaaggaaa gaacttattt tacagtcttg gaaacaagaa ggtttcaaag attttagaag
     1681 aaagtgatgc tatcttgtca gaattagcta gaggtatata cgaatgcgtg aactataacg
     1741 aagagccaaa gaagaggtaa aaaagccata tgaagaaact tgacaataag atggttaagg
     1801 aaatggaagc agaacagagg acttttgatc cctcagcgga tactactatt acctgtgatt
     1861 gttgcagtga attacctaca aaacagccac taacgacaag aaaggaagga gaagaaaatg
     1921 attttgatga tgaggagcat ggacacagcg gcaatcaaaa gaagaaacag ttgcttaatg
     1981 ataaattgct cataattatt ggtcttgctt taacaatacc aatagttcta ctggaatcac
     2041 ttcttcctca tttcctctta actggattta taacgttagc attagcaaca cccgctcagg
     2101 ttcttctagg aagaccattt tattacagat tcttnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2461 cagtaaaaca cagaaagcga tttactactg atacccttgt tgtactaagt actagcgtgg
     2521 catatctcta cagtctattt actttgctat ttacaaactc ccatgttcta ttctttgaag
     2581 cttccgcttc tgttttgaca atatttacaa taggagaata tctcgaaggc agagtgctaa
     2641 aaacaaccac agaatcatta aggaatctaa ttgctctcaa accgaaggct gcaatagtga
     2701 tgagaaatgg gagagaagag cttattgatt ctgacgatat tgcggttgat gatatagtta
     2761 tt
//
LOCUS       DAOR01000171            6463 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_48646, whole genome shotgun sequence.
ACCESSION   DAOR01000171 DAOR01000000
VERSION     DAOR01000171.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_48646"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2074 a         1164 c         1186 g         2039 t
ORIGIN      
        1 cttctaatta cgcaatagat cttaggtgta aggcttcaat cactgatgga gaatcgaaaa
       61 ctagatactg ccacaaggtg atctaattct atcgttatgg caaccggcaa ccgataacaa
      121 atacaacatc tgcttggaga aaagatgtta atcgttatct tagtatatta aagattaata
      181 atactgcgtg agtaaaagaa cagatgttag atgtattatg aaaaaactaa tagaagtggt
      241 tttttctgaa tttaacgaga gtgtatctgt agaattagat gactcacgat cacttaaaac
      301 agttaaagca atattagaaa atctaccaat tgaggtcaat atcaataaat ggggtcaaga
      361 attgtatact gataggactt caatagttgc tcaggaagaa aatgcaaaat cagaggttgg
      421 tttactagat gttgcatttt ggcctgaagg taatgcattg tgtttgttct atggtccaac
      481 accgattagc aaagtcggca aaattgtacc tgcttcgcct gttaacatag tgggacgcat
      541 tgtttcccac aataataaca ttgtagataa agttaagaat acaagtaaag tactcataag
      601 gcaacaataa caaaacatag aaagttggta tcttgttgta ctaaaaatcg attattactg
      661 cgctgaacca aatctcatgc ttacctgcag accgagaagc taggatgatg taataaccac
      721 ttcttgtttt aaaaggcttg agacaaaggc tactactaca tctcttaaag cagcctctac
      781 ctttctataa ttgcttttaa tgggatgata ttgataactt atcacattgg ctaactggta
      841 atacttagga tagatcctaa ctgttatggg aataattcct tgaaagaagc atgtagagtg
      901 ctattggatt atgtcataat aaaaaagagt atattatttt actggaaccg cgcttcgcca
      961 tgtcgaatag taccttgatg ttatcccagt tgcagttcca atttgtagaa gctgcaaagg
     1021 tggatgatat cccagttggg aaattaagac atgtagaaat tgacggaaag ttctatgcat
     1081 tatgtgatag actctcttat tcttttggtt ctcttttcta agtaattaca ccctctgcta
     1141 atagcgactg tatcatttgc tcagtttgag cataatcacc ttcgccgata tggttatagc
     1201 gtatgtatcc ttggttatcg accagataat ctcttggcca gtaattgttg ccatatgcat
     1261 tccaattcac ataattgtta tccagaatta ctggatattt tatttgaaag ttattgacag
     1321 ctgtctttac attgtcgata tttttctcat cgctaaactc aggtgcctga acaccaacga
     1381 taactagacc cttgttagaa tacttttggt accaatcgtt tagatgagga atagtgtgta
     1441 tacagttaat acacgtatac gtccagaagt gaacaagcac tacttttcct ttgaggtctg
     1501 ctagtttgat aggagcgggt gtgttaacaa aaccagtaat accagtaaat tctggtgctt
     1561 tcttaaattg agatttatca attataaaat gattttgtgt atttccattt tgtaccaatg
     1621 gtaaaatggt tggtgtcgta gatgatgttt ggccctcgct tgatgttgta gctttcaccg
     1681 tgctcgaacc taagaagtac attgttggtg ccaaaactaa tcctattgct gcgattatag
     1741 agactagtac atcactataa tttcggatca ttgacgcgtg tttcctccaa atgatttcgc
     1801 gaagactaaa cgattcgtgt atttcatgtg tctttttgtt cattcatttc attaatactt
     1861 ttttccagat tgcctccaaa ttgaaaggtt cacaaattga aatatgtagt cagaaataaa
     1921 tgataaatcg gctgcacaaa tccttggtct cattgacgac atccttcaaa ttcctcgtca
     1981 gacatattaa acaacgcgca ttattttgtt tcaacgtttt actgcgttat cgtcgttttc
     2041 tgatagccat aatcagcgca ttaatcccat aagatatact acaagcaaac tattgtacaa
     2101 tatctgagtt aagtaatgcc tttctttccc cacctaaagg atgtaatggc tatcatcaga
     2161 atatggtaac aagcttaatg ttcttgtaat agatttgtgg gccgcacaaa acataggtgc
     2221 acgtagcgtt caagctttta acgcacaggt tctcaagaat acaatttatc ttcggagatc
     2281 atatgaagct tgagacatgt ccacagcttt agaagagata agaacgacca ctgctcaatc
     2341 ttgccttcgt tactttggga tttagttagt cagttagttc tacctgggtg agtagatgtc
     2401 ggatatgcag ctaaccaatc tatgaacggc gaacttaagt ttgaactacc ctcagctagt
     2461 tccaaccaga attgttctag tgctttctca ggatgatcat ccttactacc agccagtatg
     2521 ctggcatcta gtccaccaat tgacgtacct gcgattatgt caatttttat attgctactt
     2581 gcaagtgcct tatagacccc acatccgaat gcccctaaag agcctcctcc ttgaagtatt
     2641 aagacattct caatactatc agacatgaca ctttcgctct tctttatcag gtgtacatgt
     2701 tatctctaca tcgtaattag ggctatctct aattttcaat ccttgaaaca agctgtgtgc
     2761 cttctcaatg ttagtccttg tccctttgta tagaggccga cactgctcaa cattctgttg
     2821 attgtcagca ctggttattt ttcctttcaa tctccacatt ctatatttac cagaactatt
     2881 agaatgtgtc ctattatctt tgaaaataat ctacaacggt taaaaataaa tttgaacctc
     2941 gaacattacc tttattctta aggtgttcca ttcctaaagg cgacacgtgt ttgactttcg
     3001 tttgaaaggt gatagatttt agttctttag atggtcatac taaactcgtg ttgacatggt
     3061 gaagtaaagg aatagctagg ctgtcttgtc gcgaattcag actttgggat ctgccgccac
     3121 tatcatatga tttggttttc atgctagaag acttgtagat ccttatgact tgcatcaaaa
     3181 caaaggagta gccgaccaaa ttactgcaaa aaaggttctt gatgactgta aataactgat
     3241 tctggtaagc caaacggatc tgcactaaaa ccatttgctg atgttttaaa cattagcact
     3301 tccattgact catcttttgg aaagacaaac cctataatcg ttatatgttc atgatatatg
     3361 aaatattatg gattcatttt taacatacat gccattaatc tctgtttcgg tagttgctgg
     3421 acttttcgta atggcgctaa tgaatttttc aactatgaag aaaaatatgc aaaagcaaag
     3481 tgaacagcaa ataaacaatc tgaaaataca aagtgaacag caaatttatt caagaataat
     3541 ggatgcaaga ctcaaacttg aaaatactga tgcgtttaca aaaatggcaa cggaaagtcc
     3601 tgtttttcaa gagcgattta ctgtggtaga tactcctgag gaatattaca taatagtagc
     3661 aattcttgac ttaataaaat ttatgtttcg tctatacaag aaaggtatga tagatactca
     3721 aatttggttt cgttgggaag gctacatgag aggaatgaag acaataccaa aattccagaa
     3781 ggtatgggag aagacaaagg atgtccacgc taatgaattc agagagttta ttgattcatt
     3841 ataacacatg ctagagttta ggaagttggt agagtcaata attctagaaa ttatgtaatt
     3901 ctagaaaaac taaaaaagag tttattataa tacagatcga cttagatctc catgaagtta
     3961 tagctatttt aaaggaattg gatgtctata acaaggcatc ttagaaaaaa ctaggtaaat
     4021 atgcgatagc atcctcgaat tgttattact atatactgat aagatttata gtgtatgaaa
     4081 tatagactat ttttgtgtta acccatgtga atagcacgca gaaatgaata cgaacacaaa
     4141 tatgaataac agtcagaatg attgttctct tcgcctccaa cacagtaatt gaaatctaaa
     4201 agcaaacaaa aaatctctac atacaggata gtgactcttt ctaaatatca tcagctgttc
     4261 ttgcaataac tttagagtgc aggatgaata tgaactgttt ttgtaacatt gttaatcgta
     4321 ccaccagtat gaccaactct attaccatca gccttcatag ccaccaaaac ttcatgaatg
     4381 agaattgatg cctgatttcc aaggatatga caaccaagga tatttcggct tttcttatcg
     4441 acaagaaatt ttacaaaacc gtccgtatca tcaagtgcct gtcccatatc atccatcttc
     4501 ttccagttta cgactgcaat aaggacattc actagaagat ttgctagatt taccttcttc
     4561 tggtaaacac ccacaatcag cacacttgca tgatctatat tgttatgttt gccatttgac
     4621 ttcctgcatt ttctactacg gctagtattt ttatcacctt ctttagagca aacccaatta
     4681 gtatcccagc aaaaaagcct ccaccaattg tggccggcac gagatagaag ttttcgatat
     4741 tcatctgcta ctctgggcaa gagcagtcgc aatcaataga atgatatctg cactaatgac
     4801 tttcgagcat tttttcatcc gaggaattat aggccttttt acttcctcag agaagtattt
     4861 gcaaggcaat ttggagcatt tctagatatg ttttcgatct ccttctttaa tgcatcttga
     4921 ggaagatagc cgacgatttc acctacctct ttagcctcac agaagaactt aatgaccggt
     4981 ataccttgaa ttccatatct ggctgccagc tccggttctt cacctacatt aacctttacg
     5041 aatttaatat tttctgcgta atctttcgct actgaatcaa agaccggttt caatcgcata
     5101 cagtatggac accatggtgc ccaaaaatca gcggctacta gcttcttatc cgatattaac
     5161 gaatcccatt cagaacttct tatatctgta gacatatgat ttaccctgtg tttttattgc
     5221 ttaaaagagg tttcttctat taaaaatcta actcaacggt taaagatatg gaatgtgcct
     5281 ataataatac aggctgcgat aagtaacgta gatgtttaga gaatcaagaa atatgcctgc
     5341 gggcctacct ttttttcagt tctgatatca agccagctat gtaggtgtca cggtctataa
     5401 gaataccttg ggagacctct tcatatatgc catatttcgt ttcaaaatct ttaatcttct
     5461 tcatattggt accatgttga aaattttcct ggttttcctg aggtaatagg agggcctgat
     5521 ttaatgcaga atatgagaaa gcaggcggag aagtttggca caaagtttat tgatacagcg
     5581 gttggttcag tagattttct atcaagacca ttcaagatca acgttggaga tatcgtccat
     5641 acagcagaag ctgtcataat tgcaactggt tcttcggcta tgtggcttgg cctagaatct
     5701 gaaagcagat tgaaaggtaa atgagtatcc gcatgcggta cttgcgatgg tttctttttt
     5761 aaaggtaaag atgtgggtag tcgtaggagg gggagaagca gcaatagagg aagctttatt
     5821 cctctcaaaa cttaccaata gtgtcaaagt tattcacaga agtgacgagc ttagagctgc
     5881 tagaataatg caacaaagag cgtccaacaa taagaggatt tcatttgttt ggaatagcgt
     5941 tattgatgaa atccttggtg aaaataaggt agaagggatt agaataagaa atgtacacac
     6001 agtggcactg tcaaaaataa ggtgtgacgc agtatttgtg gctattggac ataaaccaaa
     6061 tacagatttc ttcaaaggta agcttgaact tgataacaag ggatacatca agaaatacga
     6121 ggaaagtaag acaaatattg acggtatttt cgtagcagga gacgtttacg actataccta
     6181 tagacaggct ataacagcag caggatctgg ttgcaaagca gcaattgatg tagttaaata
     6241 ccttgaaagt aaggagcaga aggagtaata atttttcatc caggtttaac tgttcaatat
     6301 taactttaac tattaaagga aacgacttta tagcattttc atctatcatt aaatgaccaa
     6361 atacataaaa tctaataata ctaatgaaaa gaaaatgaag aaccatgaat atgtttaata
     6421 tcaatagaaa gctagaatca atatcaaacc tgaagacaac aat
//
LOCUS       DAOR01000172           10118 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_59665, whole genome shotgun sequence.
ACCESSION   DAOR01000172 DAOR01000000
VERSION     DAOR01000172.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_59665"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2947..3225
                     /estimated_length=279
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3066 a         1848 c         1884 g         3041 t
ORIGIN      
        1 tatctattgt gctacaagct aataattaca gacaaatgtt tatacctgtt agacaaggat
       61 ataattattg aactggttct tttggaacat actgcaatca aaatgagaaa aaggcctcta
      121 atagttgttt tggttctgcc ggtgataata tttgcactag ctttaacatg cttggccttt
      181 gttgaaacag aaacaacata tgcacaacta ggaggagcaa acacaggttt ccctacaacc
      241 aattttcaga atgcatttaa caacaatgca aatatgacag ctcaaaaacc aacaactgct
      301 acaacggcaa atactgctgc tgcccatatt gccactacaa catcaaaagc caccacttct
      361 atccaatcaa ttaagagccc tcagagtaca ttcacagaac ttgcgaaatg tctggcggca
      421 ggtacatgcc gcccaatcgt aggatctgaa ggagatgacc gtattcaggg tggtaacggc
      481 agcaatctaa taattggcct agcaggaaac gatatcattc atggaggaac tggcgataac
      541 ataattatcg gcgggccagg agatgaccaa ttgtatggtg gcggtggtaa caacataatc
      601 atagctggtg gacagggaac aaatcagatc tatggtggaa aaggaaataa tatacttatt
      661 gcaggctcag gtaacaccct tctggtagct ggtcatggaa acgataaatt gtacggagga
      721 tctggtaacg atgttttgat tggtggacct ggagcagatt actttgcatg cggggccagt
      781 gggacagggg cagccagcac aagtaaaagt gttgtattaa actttaacgc atcaaaaggt
      841 gacgatacag acggcaactg tgcaatagta ctaggtccat agaatccaat agccaaatct
      901 tggcatactt ttgatctcat tcgccaatat aacgcgctaa aatgcaagct gcggccatcc
      961 atttgtattt tttcgaatag ctttattagc tgtgctgcaa agtatatcaa aaattttata
     1021 gacaggggtg ttgcgatcca ttttgaattt tcattatgta gtagaattat gagaaaccgt
     1081 cgagctccct atcttaaatg aatcaagcat cttctggatt attggcaagt aatcggagta
     1141 tcttgcagca actgccttat agatcaacag acatgtgtcg ccgttaccaa ttgcaaccca
     1201 aatttccatt gttttaatat taagcctgtg ttcagtgttg ctgtcaagat taaatatttc
     1261 catagtgtta ttaaccaccc gtttgaaccc atgtttacct gcaagagtga tatcgctttg
     1321 tgggatgagt tttttggcta gagtctgatt caatatttgt atcatgaagt gttcccgcca
     1381 aagatccgag gcatttacca gcggtactaa gaaattagca actagatcat taccaacagg
     1441 cgctaatccc attcgaggat attcaatttg ttcccaatta gacgggtaca gtattgatat
     1501 tccatatgct ggattttcat aaatcaggta attattcgta gatgttgacg cggtagtaat
     1561 agaatattgt tgggacgaag catgggaaga cggagctcct gaagatgtca catccggagt
     1621 gttcgtctct gcatttgctt gttgcacaga ataatggggg gatggcacgc tgatagttaa
     1681 tgacaaggag taagaagcga aagagaatac taatactata atcgacatca aagaatatac
     1741 caaaagtgtc caattgccac cggttttctt ttttagcaca tttatgtcac aaaagcctag
     1801 cagttatatt ttatttgaaa gaagagagag agatagtttt ggaaagaaca aaacttcgag
     1861 aatcatcttg cacactcatt atataaccgt agatagtaaa taactgtaag ttgccaaaat
     1921 cgtcaagctt tattatgttt ggagcaatta ctatgttagt aatattgctc tccgggatag
     1981 tagtagtctt accgccactc tcagcgaaat cttcacacag acacctgaat atcccaagta
     2041 atctatttac acctccaaat caacttcagt ctgttccaaa aggacagtcg ataccgcaag
     2101 gacttaatat tcctaaaaat ctgatagtgg gaccagaatg tagtcaacag tgttcaccca
     2161 ttattggcac tcaaaaagac gatatcataa ttgctaccgc tgtcatcgac gctaccattt
     2221 acggtcttgg cgggaatgat ttcatacagt gtggacctgg ggcttgcaag gcattcgcag
     2281 gacctggcga taacgtactt atggcatcac tattagatac ttcgactgga aagctttatg
     2341 gcggcgcagg aaataatatc ttcataggag gtgctggttc tactcttttg gtgggcggga
     2401 caggaaatga tcagttctat gctggaagtg gtcatgatgt tatgataggc ggtggcggag
     2461 ccaactactt tgactgtggc ctcaatggta atggagtaat actagacttc aatcccgctc
     2521 atggtgatac taaagcgtct aattgcaaat ttgtaattac agcacatccg aataaccagc
     2581 cagcaggatc aacactgtct taacggaaca aagcatctgt cctagaacaa caatatcatt
     2641 ctttttgtca tgatcgaagg caactgatat gctcctttcc tccgtagtcg tcatcacaaa
     2701 tatttggcgc aaaaaacatc ctggataccc atctgccata tcgatctcga actcctgctc
     2761 gaagcagtta accgctttag gatactacat accggccttc agccttcctg tgaatcttca
     2821 taacattttc tattgattca gcttaggttt ctgtggtaat aactgaacca aagcattcca
     2881 gacttaataa actagtaaat gaaatgcatt tagactatgt ctaaagatgt atcgttttga
     2941 taaagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgca cattcattat
     3241 aatttttatc atacctggtc tcacttccat caaccaaccc tctgcataaa tattacccta
     3301 cccccgtccg taaaaagaat atatgccgtc ttttcctgaa ctagaagtag aaatacaccg
     3361 agactggaga atgaactttt gctataagca aatggataga tcaaacagct atgactacag
     3421 aaacaatcag tgattttatg acaacaagaa tagaggctat aagtccaact tcttctctcc
     3481 aagaagcagc aaagaaaagg acagataggg atgtttgttc attagtaggg atgatagaga
     3541 tagtaaagta ttaggtctga taactgaacg tgacatagtt aggaacgtat gcatttatga
     3601 taatgtatca ctgatagggt aaagaacgcc ggtattctat cttcacttat catcactaag
     3661 tcaaattcat cgccagaagg caactgattt atattgagaa aaagataagg catctactcg
     3721 tcgtcgacaa agaagacgca aataaacctc tacgtcttgt tacccctatg gattttacaa
     3781 gatatatgga caccaggaag ggagataacg agaataatgc tattagccgg ttattagact
     3841 actatcgaga ttagtataca gctggcttgg aaattcctcc cacacaacac cttagcaatc
     3901 tctctttttt ttgaccaaat tcacgcctaa atcattattt gccgaatcct agaagtagga
     3961 tttctaattc cttaggttat ttattatatt cactcaaaga gcgtggttgt aaaccagtgg
     4021 agctcatcga atgacttgag gggctccact gtatactgag cctcccaaga gcctatcagc
     4081 taaagtcaga tttgagagtg caccttgagg gtgtccattt ctaagtgaaa taatagcgtt
     4141 ctctatcaac tctttaacgt tctgactagt ggagctgctg gtaaaatttg cggctgcctg
     4201 tacctgctgt gctgatacgc aaagcaataa caacatcagt gcaattagta catatatcaa
     4261 gccaaatcta caccttaata gttacttcta tatactgaaa taaatagtat tgctaactct
     4321 atacgttatt cgtagtcgat aaaagatttc tagagtccat tgccatttcc atcataatct
     4381 ttctaatgga tttggacttg aatatgtcga taatttcctc tatattctta acgcctggcg
     4441 tgaaggactc cacatgtgtt cctgcagtct tacttatccg gtgagactta ctggtatgtt
     4501 gtaaattaat gataggaact acttgcctac cgttgctagt tgttgaatta aatcctccta
     4561 ctaaaattaa caggaagaat gctactggat ttcctataaa gccagaggtt cctggacttt
     4621 atcaaaagca gttgaattag tagtttttca tggttacatt ttctatcctc atgttacgaa
     4681 ctcaatgcat aagagtgagt tcgtagataa aaaaagaaaa gaagggttgc tcagggttta
     4741 ctatttctct acggggttag tgcctttgct gttgcatcca cagagtatgt tactgtgaat
     4801 ttggctccgc taagtgtatg gaatgctact gcatttccaa caaaatccat gctacctgat
     4861 gctattggac ttgcctgaat cgggccgccg ggagttgctt ggggactaag taacgttatt
     4921 gctactgcac cttttccaaa tggtgctgaa agaagggacc catgggcggc atctggagtt
     4981 acactctggt tgtatccatg taagaccact aattcgacag gtttgcttga tgtccaagtt
     5041 attgtaccaa cccaaatctt accatcactt cttggagcaa tagccatcac aagttgatgt
     5101 gcttgatgtc ccggaagtgg atcaacgctg gatgttgctg tcttttgcaa cgtgatatta
     5161 gctggcaaat ttgcttgtgc atttacgttt gctgctgtaa aggctcctag aaccaatatg
     5221 ccagcaagag aagcaactac aactgctgct atactagaaa attttttatg catattacat
     5281 ctaactcatt gtactattaa gatgtttaga aataagattc tgaatcaagt ttacattgtc
     5341 acttggagag cgtcgcatga taatattaca taacaatgct atatgtgtat atactataac
     5401 cgcttatgct agactagtaa ttgcttgaat tgttgatata catctacctt tgttccatgc
     5461 tactgtttga atccagtact aaaagagcat tttagaactg tttgaattta aatcacctca
     5521 tttttaggct cttgatagtt agacatcatt tttggaaccg tagtttctac tgactaaatt
     5581 atagggccag attatttcct gcgctaagaa atctattctc ctgaccccag aatatgttaa
     5641 aaaaatatat aaataccaat gagagagagt aataatatgt gataaatatt gaacatagag
     5701 aaaataccag acgaaattaa cgttcatcgt aaaattattg gtaaagattt aattgaagta
     5761 gattatgata gtattggtcc atgtcctttc tgtaattccc gaatagacga gttcaatttt
     5821 tgtgcatgtg acggaaacct gggcgtaagt tagaattaag atatttcact agtcgtcaat
     5881 ctcataatac agcgaattga ataagagcca tcctatacaa tgtcggtaac ttatggggac
     5941 catatttgga aaaagtgacg gtgatgataa acaatatatt atgaccctct aggccaacct
     6001 ttgtaaattg aattatcacc gtaaagctta gacaactttt aacatggtac aagtaagaga
     6061 tgctgagttg ggctattcac aagcgcagta gaaaaaacac aacgtttcaa gtaaacgatt
     6121 ttatcgtagg tggaggtact ctgcatcatt ggttagtggt gcagatggca acagtaaaac
     6181 acttacaacc gatgaatatt ttcaaaactt ttgcagagag ataatcaata gacattattt
     6241 tataagatct gctgcaatag ccgataatgt tggtcatttt atggcaactg catacagacg
     6301 acgcttaata cccttaatga cacaggagga ctcttcaagg gcggctgctc aagcagccat
     6361 tcgagcggcc acacgtaaca aatttaaatc caaggtaggc gatcttcaat tctcactcag
     6421 tagatatgaa aagttggtaa gggcaacgat tccaattaag aatggtgaaa agatcaattt
     6481 tttgttattg ttgacatttg atatcgaggc agaagccgat tcaattattc taaagagaat
     6541 ccttccttat gtagctgaga ataaggatta cttcattcag tagctgaacc aaactatttc
     6601 gatctagtag ttagtcagtg ggtatgcaca gcacgtacgt gcttttggaa agattcgaat
     6661 atgtttacta atgattcctt gactggaata gcacgtatac aaacgcagga ctaggtcaaa
     6721 agatctaatg ccatttttaa ttctgctatt tctgattcga cggtcttttt atctccttcg
     6781 tctgatgcat atttaagaag tctttcacat gcttctagct ctgcatatat tacgtctttc
     6841 ttatcaaggc aaagactatg ataagaatct ataaactgat acgtcacttt ccttttgttc
     6901 ttctctctac aaccacatgt ctttgctacg attgggaatg tcgcctctgt ttcagcgata
     6961 ttcatataga ctatccacct tcctaagagt agttttcaag tcctacacaa tcttccgaac
     7021 tgtaacccct gtggatctca ttgtctaaga ttggcattga ttctactctg tgatagttgc
     7081 agttaggaca tctaacaact gtaacacccg gggtaaggca agatgcacac cagatgcaca
     7141 attcacataa aagaagatgt ggagcaccct gcatcttatc acttcgttta cttccttcat
     7201 taagaatcag actcaagacg tggaagcacc ttgtgttatg gtacaaaaat acagtatttt
     7261 gtcgaataac attaatcaaa ggtacagctt gtatcataag aacgtttcta atctttttaa
     7321 aaaaagccta taattctaat atatattttt ttgatcatgg tattattata cttatattcg
     7381 gaaaaatgct ttcaaaatat caatttatta atgctatgat ttagactcgc accaaagaat
     7441 tacccaactt tagtaataat atctattgta tctcagaagg cccacttttt tcattggcac
     7501 tcaggagcgg cactttacta cctgagagat gtatttggac cttatacttc tttaactctg
     7561 ctcatgtatt gcgatgttga tgagtgctaa cgacaaaaat agttattgca taatgctgca
     7621 gatgctttcg tcagaaggaa aagaattatt atgaaaatag gaattatacg ctcaggaatc
     7681 tttggatcaa atgcagcaag gctttttgtg aatgccagtc atcaagtagc aataagtaat
     7741 tcacgcggat tgcaatcgcc tccacctctt gtatcttcaa ttggtgcaaa tgttactcct
     7801 atgacagtag agaatgcagc caaatttggt gaagtattgc ttcttgcaat tccttggaga
     7861 aaacgacaag aactaccttt gccagaactg tttgcaggta atatagttat tgaagctacg
     7921 aatccgtact ctttgtattt acaaagtatc gaatcttata atgcttaatt cgagtcttgg
     7981 cttttaattt cctcaaactg accgaattag tgaagtggta tttttaccaa gaagacgaat
     8041 atcagatata gaagatctat agaaataaga ttcgaaggta gtgactcatt tttcttattc
     8101 acaggttgtc ccatagcaca attgaggaag agctgggaat tgtgtccaat aaaatttcaa
     8161 agtataactc agattacaag ttcaatcaaa ggttagagat tccttgaaca tattttgtag
     8221 tttttcctgc tcatcataag ttatctcgtt tttacgtgtt ctaatcttct tgaataagct
     8281 ttcagcctta tcttgaccta atatttcctt gagtttttct tccgatcctg ttcttcccca
     8341 tgattctaat atttttacaa catcatcttt aacatcagga ctgaaatccc ttactgcgtt
     8401 ttcaattatc ctcttgcgac cgcttgggag atctttgggt ctaaaaactg ctcccatacg
     8461 gtctcattag cttaagacca ataataagtt aatcaaatat tggactgtta ccctacaccg
     8521 ctaactacta aagatacttt atgtcacact gatatattct tccaaaagag caaagacgca
     8581 ggaataacct ccggtcctac aacggataaa tcgaaaattg aagcaattaa ctattaagca
     8641 gctttttgtg attggtttaa agccacaaaa agattcgatt gaatttccat ttgcattcct
     8701 ccattggtat ttcattctat aatcgaagct atgaattaca tatagctggc tgaattaatg
     8761 tttgcatcga tttgccagat aagaggaatg gatgtaacat taaacacgga gaatgatctt
     8821 gtgattaatg atcaaatagc agaggtaaaa tcaatacatg atgtttataa tcgtactcac
     8881 ttcgatcaga acgatggtct ttcaacaaga tcggtaactg attcatttaa aatagatgat
     8941 ttggcagatg aaataagtac tcaattactt cggaagaaat ggtatgatca tctgtctaaa
     9001 gcaataggca aacagaaggg aaagattgtc tttgttaatg ccactcaatc tccagatctt
     9061 ggtcgaggta ctgtctttat tgacgagcat aatctggaga gagacattaa aaaaataata
     9121 cataaggcca tgaagtatat agataaaaat gatgtaattc ctgtaatcgt aacaatggaa
     9181 gcaatacata agctccatgt tgtttcatca ctactatttc ttcttcctat aacatgccta
     9241 aatgtgaatc cagagatagg tatgagaaga tataataaaa atttccttaa acaaaatatt
     9301 tttctttgaa ctatgtgatc tccgtagtat taattcgaac catggttggt tctggttcag
     9361 ctgtaggaat tggtgaaccc aaagcgcttg gtctacagtt ttatcaaatt cttccatttt
     9421 tgttactcgt tcctctagag tttgagttga caataaaaaa tgtagaagac cgtagcttaa
     9481 agctttaact atcgtttaat taattatact agagcgctgg tacatgggca caccagaata
     9541 tagatactcc ttgaagactt ggatttgcat gccattcaag aaggtctgag tatgattcat
     9601 cggctctgtg aagttactca gtgtagtaca aatatgattt tgaagcgtgg tgtaaaatgt
     9661 tatcgctcgt agtggcagcg tcacaaattc gtccgtctct ggattccaag ctttgaatat
     9721 tatatgatta taaactataa ctttgacgac actatgatgt tttgattgat gttgtgcttg
     9781 tccaggttct agaggaaaac aacacgtccc tgttaaatta tgaagattat catttttgtc
     9841 ctcagcagca gtcttagtct gtgatgggtc tggttgtgat aattgtgatg tttgaggctt
     9901 tccattagca tttacgtgag ccccattagt gttttcttct ttgtcgtgac ttgaactaac
     9961 tttttttgca aaaactattc cttacgtaga catcagaaga agaccaataa atgatagaaa
    10021 accgttgaaa gaaattgtta tggaaggcca atttgtaaaa taggagctgt agttagcact
    10081 gttttctatt gttgttacca ctccttagac aacttttc
//
LOCUS       DAOR01000173           13843 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42075, whole genome shotgun sequence.
ACCESSION   DAOR01000173 DAOR01000000
VERSION     DAOR01000173.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42075"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2917..3120
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4138 a         2611 c         2570 g         4320 t
ORIGIN      
        1 tgtgagttga gctacaaact atataattgc attaaaccta aaaggtacga gtaattgaat
       61 cagcttagag ttaggttatg tttgagacaa taatctcagg atgcatcatt aaatcgattt
      121 gtcctaattg gtaactatca agtatcttta ttaatgcaga taattctttt tctcgttcta
      181 cacactctat atcgtcgtgt gttcttattc tttctaactc ttctatcaat aaacccttaa
      241 gttcgcctgc atgttctcta tccagtttac ccaggcttaa taagtgcaat aaatagcgca
      301 atttctcacg tttgacatca gtatcgacgt taatgctact cacttttatc ttggcctttc
      361 cctaaccatt gtaacaggtt tcttgctaat aaatctgcgt cgactttagc cgtaaggttt
      421 ttgacatcct tctttaactg cctcaaatcg tcctctaatg aattaagctt ggtacgagtt
      481 atagacatgt ctttaacctc tttctgcaaa cctttcacaa catcgtgcaa tgaattgagc
      541 ttattacgag tcgcacctaa ccaaaacatt accccaccaa ataaagctaa tatcgtcaag
      601 ccccctgcaa ctgcggtaat agtttcagtt ggttacaggc tcatatctag aatatagcgc
      661 cataaggaac ataataaact gttaagtcaa accttaaact aggtctgatt tgggtcagcg
      721 tgacgggttt cgatatgctc ccgaatatgt tctatatact cattgcaatc tacacaaaag
      781 tccttgccgt cacgggatac tattctagcc atacctaagc cgatataagg agatgaaaca
      841 cgttgatatt aaatatcttt actcgcccaa acgatcactt aacttttgaa tatctgaacg
      901 aggtatatct attcccctag cacaattttc ccttcctggt ccagtaagtc taacttttgt
      961 gttttgctgg ttacgttcta taaatcccat cagtataaga gcgtgttctg cgatataagc
     1021 gacgtgaacc ttactagcga ctgccccaac tttgtctgca agtgggccta tattctgcca
     1081 tcgaggctct tcactattcc ttctaccttc ttcatccaat tcacacagtc cgtgaaaaac
     1141 aacattcgcc attattgcaa cgtcacgctc gtcgtaagta gtcattgagt attgcttctc
     1201 atgagcagat attttagttt atttaactcc tagtaacgtc cctgaaaagg cttatgtgtg
     1261 tataacgaca ttttcttttg attatagtct atatttcgta gcgctttatt gcaccatcaa
     1321 aatattgagc acgctgtaaa tgaagataag cgtcaagcat atcaccattt ttagcagcaa
     1381 actaaacctt ggacaattgc aagggttggg ttcgatattc cgatgacagc tgaagggtgt
     1441 aggaaatgac tgagatagac cataacaagc gtctcgcaga cgtattatct gatgctaagt
     1501 gtcctagatg taattctaat gataaggaag ctattgcata tgtcggagat aattataatc
     1561 tcagatgcaa acaatgttgg tacgaatgga ctgaaagctg gcgtccaagt atggcaaaag
     1621 aaaataaacc atcgcctaag taatgtgaaa cttgaataat caatcagtct taggcaagga
     1681 gtctgaagat tttctgtgat atcatcattg tctagaagaa cttgaaggag aagttgtagt
     1741 tgtattgatt gaacttgtat ttgttcctat agtggaggca gcagtggtaa cataagaatg
     1801 tgaaagacca gaacttccgg taaagcttca atctgtcttc tatggttgtt tatcgttaca
     1861 gttgttgttg ccgtccatat ttccatgctt ttaccttcga agacacacta taaagctttt
     1921 tcgttacata gtgatttttg ccacatatag cattcattac atatagattt tgaaaaaata
     1981 aaatttatcg aaaagaatct caccctctca catgatttgc atgtctgcat actttcttct
     2041 atctaatcaa actacttaag tagtatttcc taaaagcatt cagaaagtta tcgtggccac
     2101 ttgatcctgt aagcccattg aagaaagtta gtctggtgct ttctccgcac tagtatttga
     2161 gataactcgc aacttgatga atgagcaggc tttgctatag gtcaggattc agatattgct
     2221 ctcgatttga tacagaggga gcatagtgca gacctgaata ttgaacaagc catccaatta
     2281 agtactaagg catcagaaaa ggcatgagga gaaagtccat tgggagaaac aggaatcatc
     2341 acagctaaag acgattcatt ttggattaca tcctgtatat gtcaatccag aactatagat
     2401 cagttaggat gatacccgga ttacaaacac attacattaa ttcggaaact tacaaaaaaa
     2461 tggatattta atagagcagt gatgttggtg ttagggaatg aagcgctatt gtcaacaaaa
     2521 ctggtatgaa cctatcagaa gaatcctggg gtgtgtgtgc aggcgcgcgg ctttttgtct
     2581 tgggtgttac tctaatgtga aggagacagt tatatctaga taggatgctt gtaagttgat
     2641 atgataagag tgatcataac attatgaagt tcggtttaca gcatcctgtt tttagttttg
     2701 attacagaaa ccgcgatact tctcagattg tcgattcctt aaagaatcta gttacaagag
     2761 cagagattct ttctgggtaa tggatcattt ccaccagatt ccatttatag gaaagccaga
     2821 agaacctatg cttgaaagtt ggacaaccat ctcagttcta gctggaataa ctactagaat
     2881 aaaattggga accctcatga caggcattat atatagnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 gcattatata tagacatcca tcgattttag caaaaattgg tgcaacacta gatgtactta
     3181 gcaaaggaag acttttcatg ggaataggag cagcctggaa tgaagaagaa tcatcagcgt
     3241 atggtatccc ttttccttca gttaaagagc gattcttaag attagaagag gccatccaaa
     3301 tcatacgtaa gatgtggaca gaggaacctt cagcctcatt taatggccag tattatcaga
     3361 tcaagaatgc attttgtaat cccaagccga tacaaaaacc ctcacctcct attatggtag
     3421 gagggagtgg agaacggcag accctaagaa tcgtagcaaa gtatgctgac gcatgtaacc
     3481 tatttggatc cgctgaaaca gtaaaaagaa aactgagtat cttaaaggag cattgcagga
     3541 gtgtgggtag agattatgat tctattctta aaacgaaact tggtcttgta gtaatcgacg
     3601 acgacaaaga aatggcgaga aaaagattgc agtagatcag taagggaata cctgaagaac
     3661 aaattaacga atttgccata tatggaacac ctgaagatgt attaagacaa aaggaatctc
     3721 ttgaagatgc tggtattcaa tatcttattg ttgatttgga tccatctaga gaagttgaag
     3781 cattagacat atttgcaaac gacattataa agagatctta attcggtaac atcgtgagat
     3841 agaatcttgt tgaatcccac aaggtttctc aagtacgata tcgacagccg ttagccgata
     3901 tactcccatc ctaaaattaa gtaatatatt cctgatttgg tttttatata ctgtggccga
     3961 tccaaattag gagactttac cgtcggtcgg gttatttgca gtatgcttgc tatctcatat
     4021 tggttatttc ccttgaaaag tatcttgcat tatcctttat aatcttctca agattcttcg
     4081 atttctctaa gcggtggaat tgtaacccca ggcaatgcac ttactaaagc ttttgcaatg
     4141 tctgaggcag ttaaaataat tgaatggccg tcttaaatca tgtacgtcaa gcatgtccta
     4201 gaagggagct atctttttaa atgcagttga cttttcattg aataatgaga tttttaattc
     4261 gagccaaaat tcctacagaa gctggaaaca aaatggtaca ggatccaaac tttctaaaga
     4321 atattgaaga ctacatgagc aaagccaagc ctgaatcggc tcacttcttt gcggctgatg
     4381 gggataggac tatgggcttc atagttaata tcgaaagtaa tgaccagata cctgctttgt
     4441 tggagccact attccaagga atgaacgcca aagtggaagt gcatccggta atgaactttg
     4501 atgacctgaa aaaggggctt tcaaaaaata gataatcgca agttaatact caataaccat
     4561 tctacttttt tgaaatacac gtatgtcata aacacgatgg aattgaatga ggtttattat
     4621 gatcagctag tgatcaattg taatgctcta acaatctatg caaacattat caaaaaaaca
     4681 cgagtgacaa gtttagtctt aacatttgca ctaaggcgtt ctgaatgaat acaaaataaa
     4741 gagtatttgt tgatggttct aatcatacag tccgggccac cccaaagaac acgattagtg
     4801 aatcaattca tgacaacgac aaaacttatg acagtgatac tatagttctt gtttctttcg
     4861 gtctgcaaca tagactttct ggtctgcaga tcttattttc aactcatatg gtcgcaattt
     4921 gatgatttac ttccagatct ccgtgctgca tatacatcag ttggagaagt catgagatgg
     4981 cctgttaggc aattcgtaag tttcttccag ccgttgttcc gccgttggtt gttttttaac
     5041 taccttgtac aaccattcct tccctttctt gcttttggac agcttacccc tttcggaata
     5101 cctagaaaga tatgttgcaa ttgcactaag ctttattggt ttctgatact catcctcgta
     5161 gagttctaga atatccgatg aagtgaaggc agtatatggg aatttttgtt ctacaagatt
     5221 ccatatcttg gaacctagtg aggtaaagtc gtccttgtct ctgaactttg tggtctcaaa
     5281 atcgggatct tttccctcta tttctaaaag atctagaacc tcaatgaatt tgataatctt
     5341 cgtttttgat atgttccctt gtaacgcaag atcatatttt ccaccatcag catcctcgag
     5401 ctggatcttt atttttttac tacccattaa tgatccgccg cgttttattg tatacgtgca
     5461 aacatgtcaa ttaacgggat tactaacaaa cttcatgaca aacagcttag tgtttattca
     5521 ctcaaagcag cctcaacgca ggcgtctcat gataaggacc acttttcgga agtatttatc
     5581 tatcaataat tgcacatgga cacatctcgt gtttgcattc ttatcagaag cgttcaagac
     5641 aaatattcgc attagaaact ttcactttca ccagcttatt gtaagcatca gcaagctgct
     5701 gcaatgacat caacatgaaa agctcttcag tagtggggct tgcatgtgat cgcaatcact
     5761 atctaacatc ttataatatg gggtgttgta aacctaagtc aaaaggtcac tattatggag
     5821 tttttgttaa ttctattaat cctacgcctg aacctcttgt tcctgtactt ggtcttcctt
     5881 cacttggcct tctttctctt gttgctgctc ggatgcagat tttttcttct ttttcgcacg
     5941 ctcagccttt aactttgtta attcttcctc aaccgatgct gtcacactaa ctttttccag
     6001 ctcgctttca ataatgtcgt cactcttatt cgaagtgtaa tccgtaagaa cacccgaacc
     6061 catcatctcg tccaaggctt gtgcttttgc tttcattttc tctgttttat cttctgctcg
     6121 gctcattgcc atacctacat cagccatttc ttctgagacg cctgtcacac tctccttgat
     6181 tcgtacttgt gcgtcagctg cagaatattt cgcttttatg acctcctttt ttgatctaaa
     6241 ttcttcgact tttgtagcca gtcgtttctc tgtttgttca agcttatcct gctctgtctt
     6301 catttctaca atctgtttat ctaggggttg caattgtaac atatttgcgt tctttcgttc
     6361 caacgccagt ttagctagat cttctctact ttggtcaatt gcgcgacgtg cttgttcctc
     6421 aagagtacga atattttccc aaagtttagc tttttgcatt tcaagctgct ttttggcagt
     6481 gattacctct gcaatacctc gtcttaactt gtttaaaagt tctatctgct tagtataaga
     6541 ataatcaagc ccctctctag gatcttcgaa tctattaaga agaatattca gcttctgctt
     6601 tactaaagtt gtaatgcgat ttgtaagacc caattgaatt cctaataata tcttgacatc
     6661 atgtactatt aaatctaagc aaattgtgac atattctaga gccaaaagaa ggataggagg
     6721 acattgcctt cccaaagata ccaaagtggt tttgcaatca tcaacactaa gaagtaagat
     6781 attaacagca cctatggaag tagatgaaga gtccaaaagt atagaaacaa gttatcattt
     6841 tacgattggg ttaaaacact gcagcttctt tatggataga cctatgtatt tctttcccgt
     6901 ctggtaagga aggcagaatc taccagatta ggaagtcatg gaaaaggggg cttattattt
     6961 gtcgcttgat gaagtatcct tatattatta ttgagtttgt ttttaaaata tataaaatac
     7021 acgggatgtt ctaagattaa atgcttatta gaatcgcacc taataatccc atgttcatgg
     7081 attattcaaa actatgtcaa caggttatgg ctcttgaccc aagggttcgt tttgctggtg
     7141 tatgtgacga taccggtgag attctgtacg gtggacagcg cgaaggtgta aaagatttgc
     7201 tctctcgaga ggaaacgaga agatcaaatt tgcaagcact ggctaggtgg ggattacgct
     7261 atacacttgc atcaaaaatt ggacgaggaa agtatgcaat ggcagaatat gaaaatatca
     7321 agcgaatcac agtaccttta gacaatgatc atctcctgtt ggtaacaact gaagttgatg
     7381 ttgaccacgg aaagataata aacgccattc ttaaattagt tcaagagtag aaattctata
     7441 ctaattgaca gcattacgag gttagattta aagagctaaa gaagactttg tcgtcagctt
     7501 ctatcttata cccgtgctga taactttctc atgatctgat aaaaaaagac agatctatat
     7561 acattaatgg atactgttaa tggaatgtcc gataacagtc ttccttcttg ttagcaaata
     7621 ccccttccag gatgtcacga gaatttaccg actgggtagt cgtgcaaaag caaactacat
     7681 accgaaaact aatgtcaaga ttgttactgt aataattata atgttcactt atttagattc
     7741 actcactata atttcccact cgtcttttcc ttcgatgagg gctagactgc caccatcatc
     7801 ccaatgaatc cagatttgct ttgtttcctc attgctcaaa ccaaatgtcg taatatccca
     7861 gactacacca gaatcgccgg gctttaatct agtataagga ttgtctgtgc taaccaactt
     7921 aatcctatct cctattttta tggctgtcat tctctacatg ttaccgcttc aatccacttg
     7981 ttatatctgt tgtctacact gactttgata cacgaaaaag actgatacta ataacggcat
     8041 tcggttaagg tgagagaaag agaagtccat actgcggtcg ctcatctttt tgtgtccttg
     8101 ctcgtgggtt tcagaatagc tgcagaatta catagaggac aataaacagt cttgataaga
     8161 aatggttcct gataagaaat gagttaggct agctactcct gtagggtggg gtgtatatca
     8221 ttaagggggg atttagattg taatggatta aaagttgata gggttattgt agtttgttgg
     8281 aaaaatagtg ttagcttact cagatttata cacatctcaa tctattccta cttattacca
     8341 agcgcagaat ctataagcat tagtatgtac tctttcggtg ttttgtcttt accttgttgt
     8401 tctttatact ttctacatat ttctaaaaga ttattgtatc tgtcttcttc tagcacaagg
     8461 agattttcat actgttcctt ttcttccttc atgatataat taatttgatc cgacctggtt
     8521 taatattact tactcttggg atttgtgtat aaaaaaaaat ttattccgaa tgtactagaa
     8581 ggcaaattga agaaaaaatg aaataattct tacccttcct cactatgaga ctactgacat
     8641 ggctagatac gtgttatatc atatagctca aattgttgta agaaaatatg aaaagcacga
     8701 ttctcttttg attatagatt cagctagtta catgcatatc tggttcttct atatcttctg
     8761 tatcaattgt taaaccaata gtaaattata tagatcaaat aagtaaaaat ggtatttctg
     8821 tattggctga catgggctcg ttcttccacc ataatagatt ggattatctg atacaatctg
     8881 aaacatcgtt gccacgaaga tctaaagttg tagtaaaaca aaacgattct atcttcagaa
     8941 taaagatgat tttaactgga ggttttctcc gcatacaaga ataagttatt cgaacatcat
     9001 ggcagggaat taatgataac tactcctcct acaattaaac gacgagggct gatgaggtta
     9061 gcaactaacc cactgcaata caggatgtga ccttcattca cggattgccc tttcttgtga
     9121 ggagaacatg agttcccgtt tagatagaat attttcaatt ctgaaatata atgaattgta
     9181 tctattgtcg cgcaagcttt agattattca tataaataat gagttatttt ttaatactga
     9241 acgcatcagt ctgtcttaac tgtgtcttta tgtcaacaga agttttccat attttgaaga
     9301 tatcactaga ataacaggga actctcgtct gagaaagcgg gggaggagtc agcgaatgta
     9361 gttgcagtct tagcaggctt ggctatgcag ggtagttgta cctataaaat ctgtggcttt
     9421 tcaattgtat ctcttacgat atgtctatga caatgctctc cctctctttc gtagcacagc
     9481 aaagttatga cagtagattg attttgcgca tttaatgcgt gtattctttc taaactagct
     9541 ttattattcc tcatctctga tagaaaggct attttgaatt cttcccaatt atactttcct
     9601 tgtcgataat tatttaataa atcgccgctt ggtgagagtt ctctaatcca acaatcaaat
     9661 ttgtttttct ttattcctct tggataaaat cttgtaatta aaactctaat tccatcgtca
     9721 ttttcttcag aggactcgta tattgacttt gttttaatga ccatatttct atataattga
     9781 cgattttgat ttagttttct ctgcttgttc aggatgagac aagtgcagca ttgaccgtgc
     9841 tcaatgataa tgaactcatg catggcaggt catcaatttc tctggagatg tttcaagaat
     9901 cctagagcct atcatgcatc ttgaatgata gacaataatc tgtacggaat atgtaactga
     9961 tgtaatcgaa attttgtctt ctgtagttga agctgctgga tattcttgtg cgttatagac
    10021 taggtcagat cattctggag taaaagaaat tcttgaataa aataccatcg gaggattatg
    10081 tatcagcggt gattgcctaa ggcttgtttt gtttgtttgt caaaaaatag cctagacatc
    10141 ttattatgat gtccatgaaa gagcccttct tatatagtag aattatcttc taatcacgta
    10201 aatgacagat tctgtacaaa attgggagtc aataattcac aagactgtta gagccaagga
    10261 cggacatctt atcggaagtg ttgatgcagt tgatgatact gctgttctga tttcgactga
    10321 aggagcaagg acacattaca gaattcctaa acacatcgta gaaagtttcg atggacacga
    10381 agttatctta aatgcacctt ctttaaaatt agaacgattc aaaggtggat cagctgaggg
    10441 tttcagtgag ataagataat aactgtggtt ttttatttat tatctactta ttttctcatc
    10501 tcaccaatca gtcgttccat gatgtatcta attctgcgat cagtaagtag ggatcggttt
    10561 gtatacctct ggaaaacatt cgtgaattga gaatcttgcc caaattggtt tttgcataga
    10621 aatgtgtcac cagtatagat tacttcagac tttacttttg tttcatacct aggatgtttt
    10681 tgacatccct gttctagacg ttgaactgtt ggcctttcta gcatgatctc tcttcgtccg
    10741 atcctagtca tgcagggtcg atcgttttat ccttctctcc actacgagct ctagtcatag
    10801 catttcctcc tcatggtctg tttaatgatg tcttacgcta gtacgaagga atggttgtaa
    10861 agttaaatta gtatcatcgc ctctagtact tacagcattg aaccatccag ttacctgcga
    10921 tccaagaaat catgcatata aaaacggaaa aacatttgaa gattgtaaag cagaagctag
    10981 gagagttacc tctcagttgg ctgaagagat agttaaaaat gaagcaggag aggtttcttg
    11041 gaataagatt ctagaagcta cggatcatga tgagttggta tataaattaa ccttaaagta
    11101 tttgagagaa aaaggctatg acataggata taacaagaat cctagggtaa aagttgatat
    11161 tttttcaaat acaaactgca aataacgaga agcctacaat attctaattg aaggctgaaa
    11221 aatgaggcgt aaaactaaag cttagaatga tttcatctcg ctgtggcaac tacgccatct
    11281 cacaaagcag attttatttc tcaagactaa caagatacat caacgagata ctgagcttca
    11341 caatacaata tctccttgtc atcatcgcat cttccaccga acaaactcaa tcgttgggtg
    11401 tgtaactcga gcgatctatt cgtcaatcaa tattgcaata aaaaatattg gtgtaattta
    11461 tctaggatca tactagcgaa gaatctgtat cttggttatg gagatttgtt aaacaagaac
    11521 gaaatggtcg tcctgtacaa gcccggaaat gggtggggat aatcaagcgc agtgacgatg
    11581 atgacgataa tatctaatat tgtgacgatg atggccgata ttaatattgg caaccgacgc
    11641 ctgttataaa gatctgaacc aatggcataa tgtgcggacg gtcggagcag ataccagaaa
    11701 atgtacaaat tcatattgcc gagccagaag aggatataca acgactttac agtctaaatc
    11761 ttaaaacttt atgctttaag gaagatagaa gtactgtagc attgcttcag aaacgaacgt
    11821 tgacccagat ttaagtgtgt agattcgttt gttaacgcat cgatagcagc agaacaacta
    11881 tctattacaa ggctcatttc attttttatc acccaattca gaggatgtac atataaccgt
    11941 taagagagag tgatgatgac cgttttagga taatgaatcc taaccgacct aatcaaggta
    12001 taacaactac aaggtctata ctgcaacagg gataattgca ataacactgc tcacagcaac
    12061 aatactattg tcactgcaat atggccactc agcacaagct cagaatcaga agacgaatca
    12121 gtcacagact aataaaacaa agtacgcaaa tgctactgct gccactccta taggaaacgt
    12181 tcaacaacta tcaaaagcct taggaaataa tactaaaata cttacaagta atgctaatat
    12241 tggcaatccc aatgccggca aagccgcttc agctcaacag aaagttacgc cagatacacc
    12301 tactaatggg cttacatcaa acccgacaaa agctccaaca aaagcaggta gtgggagtgc
    12361 caaccaaaac aaaactagtg gtacgggtaa catgactaat acaacttcta taaaaaatgc
    12421 aagtaatagt gccattacca atgccacgca agccggggaa tcgattgcaa ataaaacatc
    12481 acaggctgca ccatcaattg ggtaataaga taggacatgg agtacaatct ctagtaaacg
    12541 caacaggaaa attgttgggc aacttgagtg gaacaagttt attctgtggc aaatagggaa
    12601 aataaggttc tgaacacttg ccgttggatc gatgctctaa aatatctgta tgcgatcgtc
    12661 ggacaaacgc ttatcagtgt caaaaaatta gtaggatctg gcttactgca aatagaccgt
    12721 gaccgattcc ggccagaacc catcctgcat aattgagccc aatctgcaaa aggaagtctg
    12781 cggcttcctg aatcaatgca gtaatgtggt tctggttatt ttcgttgaac acatactttt
    12841 ggtacatgcg ggccgaaaaa tgtttacact caatttaaac gtaagtagta cgggtcatga
    12901 accagaaagc tgcgacgatg aattaacgat ttggtttact ggcactagtg ttatttccaa
    12961 gggttgctga tattttgaaa tcgtcggatt aaatatttta gtcgtagtat cgagctacat
    13021 cgccagtggc ccatggggtg ttaatggtcg cataaccgtc ttgtaatcgg gaggatgggg
    13081 ttcgaatccg cacgtgggcc caaaaacaaa acgaaggaga actcccgaat atatgttacc
    13141 acatatccca tccaatcatg tctgctaccc cttaggtgct atacattgga cgttgtaagt
    13201 tgtcctagcc ttcgcctaat attcgacatc taaatattta tgggttaatg cgtttcttga
    13261 ctatatgata ctatgaagga ttcatagtca atgccgactc ttttcaacac ggtcttggta
    13321 taaggcatgg tcatgttgtt cgatttttca aatcctattg tcctctcacc attctgaaaa
    13381 tataacattc tgtcttcgta cgtcagcact tctttccatc catgtttctc taaggcggct
    13441 ttgacttgcc accaattgtg tgctggcaat tcctattgag gcatgggcag agtgtaaata
    13501 gtcctcttct tattaaaaat ctcattcctc ttttcgggga aggcttcgaa ataacccttt
    13561 attgcgagat gcattttttg ctttatgtct tcgacggttt gcgcttccac tacgagaggg
    13621 atatccaaac attccgctac gaggccttct ggatgttgaa ccacatctac atagagctgc
    13681 ggaatttcgt cttccatcat taattcacct tcactccttt cctagacata ggtgattttc
    13741 ctgaaatgag ctctacagta ttagtatcat cgttaattac aaatttcaaa gtatgtggtt
    13801 tacccagctt ctccatgata ggcctaggaa tcgcgctctg ata
//
LOCUS       DAOR01000174            2823 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_104844, whole genome shotgun sequence.
ACCESSION   DAOR01000174 DAOR01000000
VERSION     DAOR01000174.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_104844"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2115..2306
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          906 a          555 c          446 g          724 t
ORIGIN      
        1 gtataagaga caggtacatg gtagtctaaa aattaattct gtaaaaataa cttcctggga
       61 tccaacaact aataactatg ctatgactaa tggctcaaga gaagcagtac ctattggaac
      121 gaaaggagca acaacaaatg ggtatataat acattttggt gctccgagag cttttataaa
      181 gattgagcat gatgcaacag gcgctacaga tatagcaaat tcagaaattg catatttggg
      241 atacgaaagc ggaagtactt ccgctgttgg ctcaggcggt cttaactact acggggcaga
      301 tcggagtgta ataagaggta ataatataca cgatctttat tttggcttct attctagtgg
      361 tgtaagtggt ttcattattg aaaataatca aatttatcac aatgcgaatt acggtgttga
      421 tccacacacc ggaacccatg atatgataat tcgacacaac ttagtttatg acaacggtgc
      481 acagggaatc atttgttcgc taaactgcta caacatatta attgaggaca atcaactcta
      541 ccgaaatggc aaggcagcta tcatgtttag cagaaatatg tctaattcga tagcgagaaa
      601 taatattatc tctaatgagg ttgacggtat actcgtctca caatccaata acaataagat
      661 ctataatcac acaatttcaa attcacaata tggcattaac gtgattgttg gctcttcagg
      721 gaatacattc tactctaatg tgataaagaa ctgtttacat ggaatatact cacaagcaac
      781 ttcaaacaat agcaacaata cattctatga caatcaactg atcaattcgc cttctgcaag
      841 tggtgtccaa acaaaccaat caagttttga catcgctaaa agtcagggag tgaaaggtca
      901 cagccgtcct agtcatcact agtgtatcta tgatcacccc caccaactct cgaagcatca
      961 gtaatcgcgg gctaacaagt ctgtaggctg tctaaaagtt gatagtggct agacatgatt
     1021 ggcatacgct acgtacgaaa tggacttgca gtatagtaaa tacgataact aataacgaga
     1081 gtgtggttgc ttctgctcat gaaaacggta gaatttgcaa caactttttc attatccgtt
     1141 tagtcaatct gtgaactatg ttattaacta ttttagcgtc tttcatcgta ttaaataaac
     1201 agtataattt gaatgagcag gcatacgcag agaaagccct aattaatgat actgcatcta
     1261 ttgacactat ctagccaaaa ttgagaactt ggcatcaaat aaattcatga aaaggaaaat
     1321 gaggcataga cactattccg cattcatgct ttcagggttt acgcctgaag cttgtaccac
     1381 tacgaaacat cataacataa ctaggtcgtg atatagcaaa acaatctact acgatctgca
     1441 ttcaaattat ttttattatt tgcttgtatt gttcatatta tgataacgat tactctagtc
     1501 aagattaaat cacatttatc aatatcattc tcctcttttg cacaaggact aacaacgatc
     1561 atcattctag cactcctcac ttgcgctgta ctgaataacg tggcctatgc acaaatattt
     1621 ccaattcata ctaatacgac tagtacaact acttctgtca ctcctagtca tccgccaaca
     1681 ttgcaacagc ggcagcaacc acagccaaat cagcttaacc cacacttagt gaaaattaca
     1741 tcacccatta aaggtcaagc agttcccgta ggaaaagacc tcagaatatc cggaacatct
     1801 ctagataaca caacaacgac aacaacttct ggctgcagcg tctcaattaa ggtgaatggc
     1861 gtcaatccgt atcataatgc ctcgcagaat ggtactagtg gacacagcta ttcaaaatgg
     1921 aactttaccc tcatcccttc atatactgtc gtaaaacaag gccagaataa aataactgcc
     1981 aaattttcat gtagcagtaa tcctagtgta atatcacact atagtgtaaa tgtaacaggt
     2041 gtagcaagaa cattgagatc gaactcaaca gctgccaatt attctaccat tcctgtttct
     2101 tcaaatgcta ccagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnntgct accagtcctg tttcttcaaa atcagcttct
     2341 actgcgattc tgaataacaa aaaccattat cttaaagtat tatcagtttc acttcattta
     2401 gacaaaaatt cccttcacct aggagataag cagactataa caattagagt ccttgacaaa
     2461 aactccagta ataccgttgc aggagcctcc gtagtaggaa gtattacaag cccagtcgga
     2521 ccatttaaga aggtagaagg cactacagac gataccggta aagcttctta ttattggaca
     2581 gtcaaagacg gggatacaac tgggaaatat aaagtgacaa cagacgtatc tgctcctggt
     2641 tatgaaaaat actcgacttc aaaaacgttt aaggtaactc ctatccctgt tacgaccccc
     2701 caatacggtt cttttccgtc aactcatgat aataccaata gcaatagcaa tagcaatacc
     2761 aatagcaata gcaatagcaa taccaatacc aatagcaata ccactattcc atccacaaac
     2821 tca
//
LOCUS       DAOR01000175            9360 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_5796, whole genome shotgun sequence.
ACCESSION   DAOR01000175 DAOR01000000
VERSION     DAOR01000175.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_5796"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4480..4683
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8015..8226
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2760 a         1712 c         1652 g         2820 t
ORIGIN      
        1 agaagatgta attaacctat tcgacatata tttctccgca tgttctagcg catttgtttc
       61 aatctcagat aaagaagctc cagtcattgg tttgaattgt aatgcgttaa ccacggcctt
      121 tcctccgtag tgattgttaa aatacacccg aagaatcttt gtttgttttc ttatttgatc
      181 cactttctcc acccacggtt tcaattcttg ttttgaatac agataattgt accaatagtg
      241 tcccttggca tttcctccat gaaatcttac aagggaatgg tctgcggtaa cgattacatc
      301 cgacaaatat tggagatttt cacttggagg agaatctgtc aacacagctg caatattgta
      361 atgctttagc atttcccacg ggccttcagt gccccaagaa ggatgtctaa attcaacagc
      421 gtactcatag ccatcagagg taggtaatcg atcgagaaat ttttcaatat tcttgaaatc
      481 actaacggta aagcttggag ggagctggaa taatattgcg cccaatttgt ttgttgtttt
      541 taagggagat attttatcca gaaattcttc aaaagaggtc atggctcctt tttcgatatc
      601 gagtcttttg acgtgggtaa ccgtttctgg cacttttata gaaaattgga atttctccgg
      661 ggttgctctg ctcataccga taaatgtgcc cttcgtcata tatgagtaga atttttcgta
      721 aaaagtagaa tccatctctg cagtattaaa gaattgagta taatatcgca gtctcttggt
      781 atccttgtct gggtagaata caccagtcca tcctccttta tctgcattat caggataatt
      841 ccacccagaa cagcctaacg atagctcgcc catattgctc tacctattag ctcagcttcc
      901 tcttatcaat ttaagtcact taattttgca ttaattcgtt tcgtgtcatt cacagagagc
      961 atatttccgc tttctacatt cacttaatat tttatggaaa caagagtctg ttttcttctt
     1021 aatgaatact taaaccccat gatgaatgaa tctttttata agaatatctt tgtttgagct
     1081 gattttgccg acttatcaga gactgcaatc catcatagta agaacgtatt gcgtatattc
     1141 taagtactaa ataagaaaca atttcctgta gttcttggag gcgattgcag tattctgatt
     1201 ggtaatcttt tggcattaaa gcgaatcggc ggtagtagta gatatggctt attctttatt
     1261 gatggccatt ctgattttta tcaacctgaa gcatctccaa ctggagaggt agctgatatg
     1321 gagcttgcaa ttgtgtctgg tcgaggtcca gatattctaa ctaatattgg tggcctcaaa
     1381 ccgcttgtca gagacgaaga cattgttgta tttggctgcc gtgatgaaga acaagcaaca
     1441 agttatggaa gtcagagcgt acatgagacg aatatgcata tctttgacca ccagcaagtg
     1501 cgtaaatcag gaattacacg agccgcttct ctggccatcg aaaagttgat gaaggatgac
     1561 ctcgttggat tctggattca ccttgatgta gatgttctgg acgaggtaat catgccagct
     1621 gtggactatc atttagatgg tggactttgt ttccctgatc tgagcgaact tttgagaatc
     1681 atacttggat ctgggcgtgc tgtcggaatg gatattacca tcttaaatcc cggtctagat
     1741 gtagacggtt ccatcacacg cagatttgtt tcaagtgtag tttctgggct aacctaaagg
     1801 gtgtttcaac ttcacccttg aaatatatta caacttatgt atgctggaag ttgtacaaaa
     1861 aatgattgat tgttacctta cttacttgta atacacgaac tgacatgtta tttggatatc
     1921 cgaaggtgat aacttgatag ctagcaatga gttcgaccca tcctaagata atatcataat
     1981 atactatttt ctacaatatt attagtaagt tgtctccgat ttctcaagtt ttctggttta
     2041 tgatgcagat aggtatgact aacaactgct attctgacat tatggtgtaa aagagctgct
     2101 ctgtaatttc acaaaatcgc ttgtacgtgt atgattctag cttaacaata aaagaacgtg
     2161 gaatgtaatg gtacagatat cttttttgat tttattactc gtagcaaatg ctgctctaca
     2221 taatacgtaa aatttttgca aaatgtcata tgatcagcgg acaaatacat cacaaccatc
     2281 attcagtgat tggtaggcgt ggcctcctgt acaatttcat aattattggt cacattccct
     2341 tggtagtacc tattaacagg ctcccaggta aatacgccat tattatctct tactccccaa
     2401 aaatcataac ttgcatattt tctatcccct gcttcgttta aggttgtatt gccggtaatt
     2461 cccgtgtacg aattagccgt ttgtactaat gtttttgtca aagtgttgat atcattcgtt
     2521 gctcgagacg cattttctgt tagagctgct atccataaag aatcatatgc tacgctagca
     2581 taagacctag gaattctcgc tatcatattt tgaatttcag attcaaccaa gttagtttta
     2641 ttattgtggc taactcttat cgcgtagatg gggttaagga aacttgtctt tgcggcaaac
     2701 ttggctgcct caacatttct tatcaggccg ctatttagaa cagagccatc gctgccatac
     2761 catcttactt ttgagagact cggaagatct tgtgcttgaa tgaaaattgg aactacttcg
     2821 tcaaaggcta caatatatac tccgactttt tcagttccgt atctcgatat tgcttgagta
     2881 accttagagt ctaaagattt caactgctga tcccacaaca caagatttat tctatcaagg
     2941 cttgcagaga aatgacccac aggtggagca tatccaacgc catcaacaac cgtcccacct
     3001 aacatctgaa aatctccttt cgttgaattg accaggcgat ctccataaac gtctgctctc
     3061 cacataggta tgactactct tataccatct ttccgcatct gcgtagctat tgcttcggct
     3121 tgatgagtat catcaggtac cagtcgaaat atgttgtctc ctgatgtggc aagaagaggt
     3181 gcggtactag acggacttaa tagaataatc ccattattat tagcataatc ttttattgct
     3241 tctagttctg cacttgtaga aggaccgata actattctta taccttttga agccaagtct
     3301 ttgagctctt taataccgaa cgcagggttt gtgtgagtgt cttcaataat aagtccaact
     3361 ccaagattag tatgtgtttt cttgaaagat tcatttacat ctctgagggc tacttttaga
     3421 cctgcttctt ctgactcacc caaagtagca gaagcaccac ttagcgaaag tagagcgcct
     3481 agtattatag tatgttttga gcttgcagac gcaggtatta tctcaatatt tgcattgcca
     3541 aatgagaaga ttcttggata gatgaaatag accacgaaac ccgcaaataa tataagaacc
     3601 atacaaataa ttgttccaat tggtattctt ttttttcttt tttcagaaac agcaggagga
     3661 ggaactatca tatttgaatg accattgtgc aatctagtat ccgaatttgt cattttacgc
     3721 tgctgctgag gctgctgttc gtattcgtaa tggtgtaaag tcaagtattt gatataccac
     3781 accagtcctg cagccataac tagaagatgg gcagcaaaaa aaagtgaaga taaccaaaat
     3841 tgttctgtca atgatgtgag aacaactaaa gcaaaccaac tatctgataa gacgattaac
     3901 aaaagaccta aagattcgca tatccatggg gtgaaccaca aaggttcctt tcgaatatcc
     3961 agtaggatta cgattgcagg aaccattaat attgcatcca atgtaggata ggcaatcata
     4021 acaccaaaca tggcaagact tctatgagta gatagaactg ataggcttgt agtaacccca
     4081 attatatagg tgacaaatat tacaccacct ataatacttg caataagaac ttttttgcta
     4141 aatctgaatt tcttattaaa ttccttgtaa gtggcaaata aataataagc caagaatcca
     4201 taagctgaca accacagata atccgcaaca gatggaactg gcgccactat ctgccataca
     4261 atttcataca tggcccagat aatctctgca caaagccaaa gagacaaacc tatagcaagg
     4321 gctgcatgtg ctttgccatg cagagtacga aatttctgtc tgaatactat gaaaatagct
     4381 aaggctgctg ctattgcaga attaatattt attatccaat tggaataaaa gtctctgctc
     4441 ttatctgtag caaggaaaat gatagaatct gataaaattn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnncagtgaa aagaatcctt ctattgagtt ccactatatc tgttaacctt gtcgtttatt
     4741 tcgtttcggg taaataaaag ctagctaaac aaacaccgac ctcacttttt cactttatta
     4801 tcattacagg gcaatgagca taagctgtaa catctgaagc cgtgcttcca agtaccagct
     4861 tcttaaatcc cgaccttcct cttgtaccca taacaatcaa atcaatacct tctcgttcag
     4921 catagtcaac aattgttcct gctgtagaat tgggactcac cattatgtct gtctttactt
     4981 gaacattatt ggccttgctt ttttgctcta tggtatcgaa ccatttttgg gaatcttgcc
     5041 tgactgtctg aatcatcgag tctacagagg ttggtgttac tacttcggta agattagaag
     5101 tgtattggta tgcttcttct gatcgtataa catgcaaagc aataagttga gcattatttt
     5161 gtttggcgat taataccgca tagtctgctg catctattga taattctgat ccatctatcg
     5221 cagctaatac ttttgaaatt tttgccttta aattcgacaa tttattgcaa ttgtttctac
     5281 agtatgatat tataaaaacc tattatacta gaataggttt gatcatgtcg tcaaacctct
     5341 caaaaaatgc atacgaaaat ctgtgattga gaaatagtac atttagtcca tctttcatca
     5401 tattttctat tgcaacactt acatcgtctc tattgattat tggtaaatac gatagcgagt
     5461 ctgttataag catcaatttt gtatactcat aattatccat aacatcatat agaatagact
     5521 ctagatatcc aaaaaattgg agatcgtaat tgttgatctt aggcctcacg actcctacta
     5581 gaatctgatc aaactcatcg cctgtttctt ctgcttcttt ataagctgat atagctgaag
     5641 agaaactacg agatccttta tcaaactctt tgtcaaattc gatattagaa tattcgttta
     5701 atctgaagaa ttcttcagca ccttttagta aatgatgttc agttaaccaa ctatcatgtg
     5761 ttctattatt attcattctc gcaaaatagg ttcttgcgtt taacatccga ggatgagaca
     5821 attcccatag tacgtgtgta ccgtactcta atatattttt caattacccc ccccttatgg
     5881 aatagttatg aaatactttg gcgcagatat ttaaatatct cagcgaggtt tcttacttat
     5941 tattgcctcc aatgagtgta atctcttctt caattcatta atttctcttc gtagacttgt
     6001 attgccagag cagatcctta tcgacgctat gttggttttt caaatgatct gctatagtca
     6061 ttccttatta agttagtcag actggtacaa tcaagttact tagaaactac tactgaattt
     6121 aaataagttc agtactgtaa tgtctttatg aataacgaag gaagtacaat tttagtaaca
     6181 ggtgcaactg gaactgttgg aagtgaagtt gtaaggcaac tctcaactaa aggacaaatt
     6241 ggtatcagag cggcagcacg ttcagctaat aatcccactt ttaaggacct aaagagtgtg
     6301 gaaatagtgg aactagatta taataaacca gaaacgcttg caacagcttt caagaatgtt
     6361 aataaactct tcttattgac tccgttccaa tctgatatgc tagatcttac atctaacttg
     6421 gtaaatgcag caaagaagtc aggtataaaa tatatcataa aacagtctgt tatgggagca
     6481 gatgctgagc ccggaattac tcccggtcgt ttgcatcgac aagcagagaa aattatagaa
     6541 gaatctggaa ttccattttc attcttgcgg ccgaatttct ttatgcaaaa ttttgtcaat
     6601 tttaatagtc ccacgataaa atcccaaggg gccgtatatg ctccagctgg agatggaaaa
     6661 gttagcttcg ttgatgtccg cgatattgca gcggtagcag tacaagcact aatcaatgac
     6721 aatcaatata aaggaaagcc atttaatatt acaggtcccg aagcactctc ttacggacag
     6781 gcagcagaaa tactttctaa ggaattaggt aaggaaataa agtacgtaaa cataccagat
     6841 gaagatgctc gcaaagggat gaaagagatg ggtatggatg attggtctgc taattctctg
     6901 atagatcttt ttgacattac tagaaaaggc tatgcttcag acatatcttc tgtagttgaa
     6961 gagttgactg atagaaaacc aatatcattc tcacagttcg tcaaagatta cgctcaagct
     7021 tttagatgaa actgtacgtt atctgtagga tcatgaatac aaaaccacag tcaacgagtt
     7081 ctgtctactc gttgttgttt tgtgaatatt aataataaca tccgaagctg actaagtgat
     7141 gacgttgaag tcgtgggcct aggtgctgaa acatctatgg ttgaaacaca cactataaca
     7201 acatataata cggttgtaga ggattaactt tggaggttat actatgacct acaataaact
     7261 aacacctgaa gaaaaaaaag tcattataga taaagcaacg gaaactcctt tctctggcga
     7321 gtatgataat ttttacgaag acggaatatt tatctgtcga aggtgtaatg cgcttctctt
     7381 ttcatcgaaa agcaagtttg attctgactg tggatggcca agctttgatg aaaacttccc
     7441 aaatgcaata aagcgcgttc ctgatcctga tggaataaga acagaaatag aatgtgctag
     7501 ctgtggtgcg cacctaggac acgaatttat tggcgaagac ttgacagata aaaacactcg
     7561 ggattgtgtg aattcgttat ctatccactt tatcccaaaa tcagaggagt taccaaaaac
     7621 gatacatgac gaataacaag actgaaatca caacacttgc caacggatgt ttctggtgta
     7681 cagaagcaat cttcaaaaga ctcacaggtg tcaaatctgt tctacctggc tatgccggag
     7741 gcacagtaaa aaatccttcc tatgacaaag tatgcacagg aataacaggg catgctgagt
     7801 caactcagat agaatttgat ccgaaagtta tatcttttga aaaaatcctc gatatattct
     7861 ggcatacaca taatccaacc actcttaata gacaaggtaa cgatgttggt acacagtacc
     7921 gttctgcaat cttttatcat gacgaaaagc aaaaggagat tgctgagaaa tcaaaaaaag
     7981 agcttgaaaa agaaggtggc tataaagatt ctatnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnttta tgttgcagag gattatcaca aaaattacta tgaaaaacat caagatgccc
     8281 catattgtaa ttttgtcatc ggacccaaaa tccacaaact gcttcagcag tatggaaatg
     8341 acgtaaaaga agaatataac tagcctttag tggccaggag cataacatag acagcaccgt
     8401 actaagcaac atctgatgga gcagccgact aactagaata gctaagacgt ggtattagtg
     8461 ctgcttcagg ttcttgcatg tttgtttgtt ggtcttttga gctcaggctc ttgcgtgttt
     8521 cacagctcat acagtaatac ttcaatgaag aattattaat gctgccaaac attgatcccg
     8581 tcatactgcc cgttttcttc attatccttc tcatatacaa atttaccaaa atgaatactc
     8641 cgaatagcag ctatggatat tggaatgaca ataacatgga gatcgtaaga cttattccaa
     8701 gggatactcc taaccatact aattgattgg taaggaattg tttgttatta cttatgctca
     8761 aattcgattc gcacttaata tttttgacat gcagttgtaa gaaaatttcc aaattgcgtc
     8821 catattatgt ccattaatta agccaatcat gctaacttca attagttaat tgcaatgctt
     8881 tttaactcta ttgcaataac aggaaattat tgcaacatgg cactacataa gcatacttat
     8941 agcacgttag tctggttgtg tacgtactat gactctagac atccatttgc ttgataacta
     9001 cgttttgaac ggatgatccc agaacaatta aaatcttgtt tttcaagctg agcactgtca
     9061 tcatcatcat cttctcgtca cagccacgat gtttacctct tcgagaatta gcgcaagaga
     9121 ggaagtattg ttaaatcctt atagatgcag agatatgcta ttcagaagtc gttatcgttg
     9181 tatcctgcta ttgatggttt gattgggggc agaatgccaa cagcagctat gacattgagt
     9241 gagataccat catggaagaa gtggggtctt cacggtgttt ttgaatggca cgaaaatcaa
     9301 attataacaa cccgtttgct tcatttgtcg gatgcgaaaa gaaagaagaa tcatttcaaa
//
LOCUS       DAOR01000176            9834 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_65140, whole genome shotgun sequence.
ACCESSION   DAOR01000176 DAOR01000000
VERSION     DAOR01000176.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_65140"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3209 a         1768 c         1798 g         3059 t
ORIGIN      
        1 gtactatatt ctgttgttaa tagtgtaagt ttagaggtag ctccataata gtcatatctg
       61 ctatttattt tagcataaaa ccttggttat gtttgtaata ttatgcaaaa cttctgtttt
      121 ttctatagtt gcagatggaa ttgacaataa ataataagta aaactaataa atctataaat
      181 gtatttttgt atatattctg aggattaaat aaaatacaaa cttcttaaat gattaatgaa
      241 aaatatagat aatagcccct aatttcgtaa cccagggtac aattatttaa aaattagaac
      301 gggtcatatc gatcggacta aggatgtgtg cttattggat tgatctttat tgatacggcc
      361 gttatgtggc aactcatcca tttctcgtct gtagcttttt atgtaaaact gttcgtcgta
      421 tcagccgtat ttggacgagg tgtacacgtt acacttgcaa ttgctcgaaa tcataattca
      481 agcaataaat acctggtttg aagtaagtga aactgaaata aaggggaaaa gaaaatgagc
      541 aaaccactaa ttccaggaaa tgcgatttcg tttgtcaggc ttctcagaca acactctgcg
      601 aaacatgcag agcttgcaga taaacgagtc gacagtgctg aggagctaaa acagacagac
      661 ctgcgaaaca caaacctttt actatctaac ttgaacactc aactaatgtt tgtgtattca
      721 ttgcttgata taacaatgac atcacttaat gacttggaga atctaataat agagaaagac
      781 gcaacaaaag aagaaatgga tcgattcaaa gccgagttaa atgagaggct taaggataca
      841 ttaaaaccac tgagggaagc attcggcata gaggaaggac gtctgaaaag agcagaagat
      901 ttgtacggtt agttaacata cggtccataa aatcagttgc atttcaagaa tactatttat
      961 cttgaagtat taagcattaa gagtcgtagt tatggacaga catacgaaaa aatagattat
     1021 attctcgcaa tcgcaatata tttcgttaat gtaatttcat atgtaaatat ttaatagttc
     1081 tgggcaacaa ggcacattta tcaagccttt tatgtttagc aaaacaattc aattctaaga
     1141 gaggttttca gatgataaaa attgaaagga tttatgatta tccaaaagga agtgttggtt
     1201 ttaggatctt ggtagacaga ttttggcctc gtggtttaag caaagataag gtaaaggtgg
     1261 atttgtggca aaaagaaatc gcaccaagta attcattaag agtgtggttt gggcatgatg
     1321 aaaagaaatg ggatggattt agacatcgat attttaacga attagataaa aacaccgaat
     1381 ccgttaagac tatacttgat aaagcaagac aaggatcaat aaccctgctg tatggcgcaa
     1441 aagaagaaag atttaacaat gcagtggcgt tgaaagaata tcttgaagcc agttagtgtt
     1501 atcgttgctc attattgtaa acatctgagg gtgttgttga actgcagccg accgtatttg
     1561 ttatagttga tgcatcataa gaaatacaac accaaactca tatatgcttg atactcatat
     1621 gtaacactaa gatgtcagat tcaccagtaa ttgattggga agcaattgtc cataagaatg
     1681 tcagatcgaa agatagggcc gatgtgggaa atgttgacgc catagagaca gatgccatag
     1741 taatcataac tgagggcgca cgtggtgaat acaggatacc aaaatctgag gtcgaagggt
     1801 ttgatggtgc taggtgttcc tgagatcaaa tattgctgag ttaaaaaaag tataaaatct
     1861 gagctacata tccacaaacg catagcgcag atctattgca ttacatttag ccaagatggt
     1921 ttaacaaact tggatacgaa attcagcatt tgatccatcg atcctctcta gtttgattgt
     1981 tataattcat ttcactctat ttccacgact tatgacgtga aagtttccct attccttcgg
     2041 cgtagccttt tcttttaccg gctgcgttca tgatatccgt cttactaacc agaccagtta
     2101 gctttccttc ttgatcgcaa ataaacactg tatccatgcg ttttctactc atatatttta
     2161 gtccatcttc cgctgtcctg tcagtatgca taattattaa atgtgacttt tggatcataa
     2221 tatccattac atgcaccaaa cttcttctgt gttctggcac cctccatgca tctttggacg
     2281 caaccatacc aactagataa ccataatcat ctattactgg aaatgagtcc ttcgcagata
     2341 cacttatgta atcatgtatt agatgattga taagcatatc ctttcttact gctataatct
     2401 gcgtattcat ggtgagacga acgccagata aaatagatgt cagttgatgt tgcgctaaat
     2461 atgacctagt accactattt aagaaccatc ctacaagcaa caaccatatt ccgctgataa
     2521 atgacccaga aaccgtgatg aggaagccga accccatgaa tccataggat atagctattc
     2581 caattcttgc tgtaattttt gtagatttat cgtaatcacg tttcgatctg agaagagctg
     2641 acctgagtat cctcccacca tcggaaggaa acgcaggtat gggttaatac caccaagtaa
     2701 tacgttgaca agtgcgccat attggagtac acctgctacg atgatcgctg atatattatc
     2761 agtattatta atcctactat tattgttaga tcctgtggtc acatagatca gtaggcataa
     2821 tgatgttgct aatatagcag caataacaaa acttgttata ggacccacaa cagcaatttt
     2881 aaactctacg aaaatctcta gaggcttctt cgtcttgatc ttcaatcaca gatactcctc
     2941 caaaaataaa gaggattatt tcacgtactt taagtccata ccttagtgca acgatggagt
     3001 gtgctagttc atgcaaaagt actgatacaa gaagaatgat tgcacctatg atacccataa
     3061 tccaatatac agaaatatcc aagccaggat agatttcagg catcagcatt gctgcgaaag
     3121 cccaagttgt cagaaaagag acaatgacta atgtaaaatg taacctaata ggtattcctt
     3181 taatttttgc tacttgtaaa gacaatactt atcacacatc tcagaatgtt ttttacatcc
     3241 atgtatgcta tttcatctaa ggaatattaa cgacttgatt aaccttgtaa attaacaatg
     3301 ttaactcact tggttaagca tgttttaatt caatcctctt gctttgttct taattaagag
     3361 gtgaaacaaa aaaagaaatg ataactaaaa acagaaactt gttgataatt acggtattag
     3421 ctgtaactgc gtcaataacg agtggaattt tggtcgccac atatacgcag caaggagcaa
     3481 gtgcccaaaa ccaaaacagt actagtagtc caggtgctgt tcccttgaga aacctgacag
     3541 gttcagtaca aatattccca aaactgtcgc aaatcttgca atctaaagcg aacgctagtc
     3601 taagtacagc tgctacgaat gctgaaaaat caataggcac taattcacat gctatctctg
     3661 ctcgcttagg aatagtgaat ggatctctag tatacacact gcatgttgta gacgctagca
     3721 ataccatcca tagagttata gtagatgctg gaaacgggaa ggttttgtca ttgcaacaag
     3781 tgccttttga aaatcaacta catggtcgag ggggagaaca aggtatgttc gctcatcctc
     3841 gaggtggtac atttacgcat ccgggtttcc taaggcattt tggtggaatg taatagaata
     3901 gataagcaca acttcagtag cagtattaag aaaagatagt actgctttcc cttattttta
     3961 gattagttct atctgcattg tcagactcat gcactgctct cgacatatcc ttattcatta
     4021 aaggatcatg aacaaattct gtttatgaca tttgtaatac gcaaggatat ccccctttcg
     4081 tagcttaggt ttaccaccta gcttggattt attacaaatc tgactataat cttccgggtt
     4141 tttacgcaga accaacgata gtactagttg ttctggaaga gcttgtctac tatgtaattg
     4201 ctgcaaagct gctttaattt atgaatagga ttgatatcat ttttaagctc atgcactgat
     4261 tgccagaaat ccatgtctat aaattgatgt cgatgccgta acagccagca gatgtatttg
     4321 gtatgaacca aatttgtgct tcaccgcctc agtttggtcg atttcatgtt caatcaatag
     4381 cttaacataa aatatcctac ttttgcaaat ttattcattg tcatattctt ctcattatca
     4441 aaaaatggtt taagaacaca ttactataca tccgctggag cctattcccc ctatagtcgc
     4501 atacgtctga ttgccctttt tcagtatatg tgtaatcgcc gctgttgcct tgtcttctat
     4561 tcgtgtggca accaaaattt caaagcttcc ttttaatctc tagcaacttt acttttgtta
     4621 atggctttaa caagatttct ataattatta tgtaatgaag caaaattatg ctcgctacta
     4681 tacatatgta tgatgccgga agtctggata ggatggttgg cgtataccag cggttgttct
     4741 aaacgaatat cttctggtat tattgagcct gactgcgtaa caaataatac gttggttcta
     4801 aatcctttat acaagtctgt agaacccgcg cctcctatga ctatggggca aaattcgctg
     4861 tgtaatttcg gttcatacga tggaggatgg tccccatatg tcaccttact atttgatacc
     4921 agcacttccc catcaacaca ttttccaccc tataatgcaa gtcaatttag aatgttttct
     4981 ttgtcgttga ttatattcaa attgaataaa ggagtacacc cttaccgctt tatcgttata
     5041 tttcagtgtt cgtccgacga tggttaacac ttttatgatt tgcttacaac cgacgatatg
     5101 ttaacagagc caagtttagc aatccctata agccgggaac gaaaccagtc cagcccaccg
     5161 aggtttttat gcatgcgtgc cagagcattg gttcccacac aattcaaaac taaaagaggg
     5221 aagcgcagca ccctaagagt cgttagtggt gttataatta ttgaataaac gttcaacata
     5281 tccacgcacg aactgattcg atagtgaata aagataaaca aacaacaatt cattcataaa
     5341 ccctatattt ccctttatcc ccgatcatat tatttaactg gtcgcatagc attaagaagt
     5401 gcgtcccttt ttctgttatt ttatagattg attgttctac ttttgttagc aaaccattat
     5461 ttaataggaa tggcacatat tctctcattt gtgtcgaaga cagaaaagcg ccatacatga
     5521 ttcgcgtctt ggtcgcgtct cttcctgtaa cttcccttaa aatggccgcg attacgtcga
     5581 gcctgcttct gtttcttata tgttgttgag tggtaggggc ttcttggttg ttcatgctaa
     5641 cctttgctaa catctttagt tttaaccttc accgcctcaa actagataac aatagacatt
     5701 aagattggat cacattaaca acactaccga tttttctgat taagagcagg gagttttgat
     5761 cagtgttgaa caagacgtat aaaaactcct gctgattctt tttatcgcga gtaataatag
     5821 cagtaacgac ggatctaata gccaattgga atatttggaa aaaacaggaa ggtgttacta
     5881 ttcccagtta gaggaataat cggaatccaa tcatcatctg caaatgcaca ttagttcaaa
     5941 cggcattttt gttgagttgc cttctaggtg aacgttagta gctggctgat aaacccaaga
     6001 ctatatttgc tttgtcatgt tactttgcgg atgacttttt tagattttta ctagctttta
     6061 tatggtatgg tatttatttt ttagtcgatc tatagtattt cctatcagaa acatatgtct
     6121 tacattgaca tttataaatt aaacatttca tcttgtctct aggagcagcc ctatcgtggt
     6181 caatcttttg atgtttacac tttatacacc tgacaagtaa atcagattca ctattcattg
     6241 ttatttacat tgtaacctct tattttttta ccttgttgtc atagcggacg attagatatc
     6301 agagtatctc cagttggacc tgaagcaaat gtagagtctc tctagtataa tgatcacttg
     6361 tacgcagaga tatttagaat cgataagaca acaaaatagt atcatataat agaaattgag
     6421 ataaacatta gtactaagtt tggcttctca taaatagttg tttgactcta gctaaatatg
     6481 aaatacagaa gcaggagtga catcgtagga ctactgttag atgcagcaaa tggtggagga
     6541 gctattaaaa caaaattaat gtataaagca tatctatcct ttaaccagct gagagaatac
     6601 ttgacacttt tagttgaaaa tggcttgata gagtatcaag aagggatgca gacttacaga
     6661 acaacagaga aaggcatacg tttattgcaa atacagaaca caatggatga aatagctcct
     6721 attaaactac atcaataaaa aatacggtaa acaaagggct gctgacccag atgaatattt
     6781 caaatggtct acagtatcac caacaagaca agaagaaggc gaaacaaaat attaacacta
     6841 atacagaact acaatttgaa attgaatgta cacgttgcta tggtgctatg attctatgtt
     6901 ctgattttaa tagcctatac tatctctgcg aagaatgtaa ctttgtcttt ataattaatt
     6961 ctacttgtta gatagttcaa atcattcagt tttctaaaat gaaaggctgt atttggatca
     7021 aaacaagata gtttaggata cgataatacc attattatat catttttgtt gacctcttta
     7081 catatatcta tcttacacta ctaactattc tttcaacaaa agaactttgt aagtattggt
     7141 cttgagtcca acagtaataa cagaatcaga cataaacatt catgtgctcg gcgtccaagg
     7201 gaagaaaaat ttactaatgt caagaacatc atagaaatgc aaataatatt gaataaagat
     7261 ttgactcaag aagaggcgtg gaagattatg cagccagttt gcagggaatt actcgagtta
     7321 gtagatggag gttatctgca atttgtatct gcgagttaca atgaaagtga tggaacatac
     7381 aaaataaatt tgaaatcctc tcacatacac cttgcatcaa ggggattcaa ggatagcatt
     7441 ggcgatgttg aatataatca aggaaaaata aggatcggct tacgagcaaa tgggattccc
     7501 gtgaatatct ttgtagacat tatatgacga agtgaaggac cggtgtgcta tatggaacaa
     7561 ctgctaaaaa cctaactgag ctcaattctg acctggaaat atattcgtat ataacgacac
     7621 aaatcacaaa acataaagct ttcctcgtgt attgaagaac atagtatgga accttctaga
     7681 tgtgaatttg aaagtgtagt aaaacaagtg tgtagaaaca aggaatttga gctctcaagt
     7741 gggaagcagt ggtaacaacc ttgaatggcg cagaaacaag ctacgcgtcc cgtaaggcgt
     7801 cctttaccta gaatttatgc aaatattaaa aaatattctt atgcgtttag agaatcaggt
     7861 aaactgggtt tatcattcct ctaccggagt ggattattag gtacggctct tccgattaaa
     7921 accggaagtg catgtaaata catagacgaa taaagaagag cagcgaacac gataatcaag
     7981 gttgtaaaaa taaggattgt agtgaaacag ttttcttaac gagttacttc tttttggcta
     8041 acctgcggag ctcttcttct tgtttctctt gttagtatta tagcagcaat tataatgaaa
     8101 gcaatttgaa atacttctgt tcctattcca acgtatttaa tcgataatgc tcttgacgta
     8161 attggattat gtattcttgt cattccatat aggacaattc gtgctatcta agtatgtatt
     8221 taatacatag ttataaaggc gtttaatgag tatctcttga tgcaaactgt taatgtaaag
     8281 acatgcaatt acattaacga caacgatcct ttatttgaaa gaaattcatg ctacagagtc
     8341 tgatattcgg atggtcaaat aaaatctgga aagatatgaa acaggaatta tgatatcaat
     8401 ttgatcataa aaataaagga cagtttcagg ctgctttatg ttaaagagtc tcttactttt
     8461 accttagctt tgttgagggt ttctgaacta ggcttaccct ctgcatgatc ttcttcagtc
     8521 ttcatttttg cctcagcatc ctgacttgta ttttttagtt ttttcgcaat atctgtcatg
     8581 taaactatgt atagtagaca tagtttaata cactttcata agaacaaagt aaaacagagt
     8641 acattgaaag aagaaccttg ataacaaaac aatattcgga ttttgatagc agtattccct
     8701 tatatacaac accagatgta ggacctgctt ttactttaga tctacatcat agcctccatc
     8761 agctagagga gcaccttgat gggtccaaac attgccaatc ccagagtatt tttcctcaac
     8821 attacggaca ccgataattt cactaccaac ctcaagtgtt tttatctgcg aaggttgaat
     8881 atcctctgta tcaacatctt taacaaaatc cttactcagt acgaagaaca ttgtcatttt
     8941 ggaatttaag gtctataagt tttttcatcc gaattactct cacgcggaaa tatgtaattg
     9001 gcctaaaaaa aaaagcgtca aaagtgtttc ctccttattt tccgtaagct cattcatcat
     9061 aatatattaa attgcctcct tcaagtacat tacaagcttc tggtacagct ggatgaattt
     9121 gaagagcaca tttgcgggat gaatcatctc tattaatgaa tatcttccgt catcggcttg
     9181 tgtggtgtaa tcagcctaac ttttagatat ctttttctta gtacacgaaa tagcgcagca
     9241 tcttgcattg atacatcatt ctcttcttgc aagccagttg actttgccac agctgattcc
     9301 tatgattcct cagcattaac acatctagta attgcaatat acttaatagc aaatcgctga
     9361 tcataataat gatgacaaaa agaataatca tcggaacaat gtcgctccta attaaacatg
     9421 ggtatgtgat acatgaggca tgagccagca ggaacaaacc agtactgaaa cagtgctgat
     9481 agtgaatcca aattcttgtg gcggatcgac tggcaagaac tgggagaatc tctataatca
     9541 gatcaaggaa agttttggtg aaaatcccga ggttgcattc agccaaaaat ctggggatgg
     9601 aactattctg acaagggatt ttttaagaaa gggatttaaa aaaattgttg cattaggagg
     9661 cgatggaacc ataaatgagg ttgcaaacgg tttctttgaa gtgataacat cagtaaacaa
     9721 taggagtaat gataaaactg cgccggcatc gcctatcttg aaactgataa accctgaagc
     9781 aactatgggt ctagttccat ctgggtcaag aaatgtacta accaaatcct taga
//
LOCUS       DAOR01000177            3063 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_94585, whole genome shotgun sequence.
ACCESSION   DAOR01000177 DAOR01000000
VERSION     DAOR01000177.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_94585"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    372..556
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1257..1500
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          797 a          504 c          435 g          898 t
ORIGIN      
        1 tgagatgaag ttaagtgcta atggtgcgag aataggcaca aatacaaatc tatctacctc
       61 ttcagctata tctattgaga tgatgcttcc ccgtttaaaa ccgccattca atcgctgatc
      121 tagggcaata cttcctgtag agtagtatcc cgccttatga gtgacagcga caaaatgttt
      181 tgctccatca acgctaattt tatgtgataa cttcgaaaat gttctaaatc ttcctccttc
      241 tagtgtaaac ggtacttttg ctcttgatat atgcgtgcct tttagctttt gtatctccat
      301 ttctctaagg cggtaggatt tatagaagag ctgtgttaaa agaataactc catctacgag
      361 atactctatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnatct acgagatact ctatcgtaga gttactcaaa tcttctgtta
      601 gaaagatgat aaagccttcg tttctatcaa tcaaggatgt cagcattttc tctgccctta
      661 ttcttgtagc agcatcgact tcctttgtta taccatccca aacatcgagt atcacaaagg
      721 gcttggctct ttcatttaag gatacaaacg gatcttcaat cttggtagta agattcgtaa
      781 tagaattaat gatgctcatt attgtaaatg aaggatccgc aagcatgaaa tctgtgccag
      841 ttattgatat aatattctgc gctttgacga aattctttac ccaaggaaat ctattataaa
      901 gggcttgttc ggatgtctta cgagaaataa ataccacatc gaatttatca ctatgggttt
      961 tggctaattc aaagcataga gtggccttcc ctgttccaga ttttcctttt ataagcaaag
     1021 acctatttcc aatttttaca aacttatgta gatcgtctag cccctgatct aacttgagca
     1081 catcagtcaa tttattgatg ccaatctatc ttccttaata ttaaacatat agggtaagga
     1141 agtcaaatac cgaaaagaag atgtttttga attcttgagt tagaagatta tattagaatt
     1201 agtcgtgtca tttattggaa ccgctcttac cgtatcttct tcgtcctcat ttatatnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 tatcttcttc gtcctcattt atatcttctt cgtcctcatt tatatcctaa agcattttag
     1561 ttgttaccct tcgatagata tattgcataa ctctcttttg tctcatgcat tatgtatgat
     1621 tcagcttgtt ggaacttctt cttaaggttc ggattaatgt tgttgaagac tttatctccg
     1681 attacgattt gatttgactt ggcttttccc atgatcttcg cagccctact tattacatga
     1741 cctagaagat ctggatccga tgcaagaaca acaatttggt tttcgcctgc atcaactgct
     1801 atcctaattt ttagtttggg atatctgttt tcgaaaagaa ccacattgat tgattcctcg
     1861 atcattcgta gcagattaaa ggcacaactg acagcatttt cacaggcgtt ctggatatcg
     1921 ggttccttag gaaaatatcc aataactgca tctcctgcat acttcaaaac gtaaccgttg
     1981 tgcttggata tcattattga ggtctcctgg gaaaacattt taacaagttt agccaattgt
     2041 tctggtgaga taattgcaga caaggctgta gaagccacta aatctatata caggatcgcc
     2101 atcacttctg ttttattttt gctttgtaac aaaaatctac gtgatagata tgctatgaag
     2161 atttgagaat aagagtctct atctagggag tctatttgtt taaaatttac tgctagcctg
     2221 cgtcgacttt tatcaaagaa tgcattcagc ttaatatccg atagggaatc agcttgatgt
     2281 ttaatctctt caaataaaag aataagaaca cgttctggaa taaagacttt ttcaacttta
     2341 tcataatacc ctctctgtcc cgcaattact tgctcttcga acctttcttg atcagtcagc
     2401 gtcttgtaat cggtttcttg ttctatttgg tcattgtcca tttagtttat ttaaccttga
     2461 accattagat aaactgtaaa gatggataag aaaaatgatc cgcactgaac atagtacgta
     2521 ggctctatct atatataatt caaatagaat tctccttaat aactacgaaa agaaagggtt
     2581 gtgtagtgcc tcaatttatg acaagtacag gacaataagc gtaagtcaca actcctcgta
     2641 caatgagagt aaagaaatac cggacagtta agtgtaccta gtctattata aacgtctgag
     2701 attctgacaa caggctaata ctatgtgcta gaatatattc acaagcaaga atggagctct
     2761 tctagcatcc tacgataaga aatcctatca tcattttatt ctagctacct aaatttttat
     2821 atttgcgatt cctgttccca gatcatgatc attatccatt tttagttttc ggtatatcgc
     2881 tagttgaata catgacatat ttttaagaca aattcaggaa agtcaataac ataagttcat
     2941 caaaattcat tcaatttatg caatttcata ttctagataa gacagatata gaaaaagata
     3001 agcacccagt atcactgtaa gtaccattta ccaacgatag taggacaact agtagcttca
     3061 aca
//
LOCUS       DAOR01000178           13782 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_37133, whole genome shotgun sequence.
ACCESSION   DAOR01000178 DAOR01000000
VERSION     DAOR01000178.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13782)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_37133"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    7009..7195
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4105 a         2710 c         2705 g         4075 t
ORIGIN      
        1 gattgtgcgt tatgatatct gtggcccagt caagaagaat tttcagtctt taatattcag
       61 tgagtcactg atatagtaag attgaatccg gggcgctttt acatcacaag agcatttact
      121 aaggtaatat ttcatctcac tttccgaaca cccaataccg ggagtcatga ttataaaacg
      181 caatacttta actccaaaga tgactttgga tttagtatct taaactaaat ctgatgttaa
      241 attattagca cccactcttt aaactgatag tttcttgaac aagctgcccg ctgtaaccga
      301 accttcagta ttacattagt tcacatttga catctttttt ttattgccat tccatagaaa
      361 agaaactgtt tgacaaatat atggttcgat tttgtcctag cctttattac gtataacttt
      421 aagttttgta attgatttat gcgtctgtat ggtcttgagc cggggtctgc acgtgtaaag
      481 ttgtttattc gatccacctt ttcagaagtg gcaataattt attttttatt aatgagtcat
      541 gatctgcttc gatattaaat gagaccatca gcactggata ttcgtcattg tccaatagta
      601 tcgtggttcg ttttactttt tcatacaatg taaacgaata aatgggtttt ccaagcttcg
      661 attgcaggtc acccctagtc gtcattctca atacagattc aatcgttgat aaatcggatt
      721 catcttcagt caataaatgg tccgatttct ttcgatatgc agttttaatc gtttttccca
      781 ttttgttggc gatacctgcg aatctaatcg aacggtccat ttttagtatc tgaacgcaca
      841 gaccatctag gctggcgcca ttgcgtccag attttttagc cacgctacat atgtagatct
      901 tgcgtttaat tatttttggt tcagcaaata tttagattgt gcgttatgat atctgtgggt
      961 acacatgtac aactacaaaa ttgccctgca atagttagtg ccccgatccc gtctttactg
     1021 taacgtggga acaagcgttt gtcgtccaag aaccagtcaa gatttaacta aacacattca
     1081 cattatctag cccatttctc ccgttggctg aagattgtgt aaagacccat atgcctcgcg
     1141 caagatgatg gttcctgtgc ccagtgcaac ttacacaaag ccctcttttc ctgctgccaa
     1201 aattttctga gttcaacaaa gaaagttaga gcggagtcat tttgtcaatg agcctcgcat
     1261 tagtgatctg tccattcttt tcatctatca tgagatcaac tgtatattga acaccatttg
     1321 acaaaaaagt cacttggacg tcccaggaat ctcttttgtg ctcggatgga aatgggaata
     1381 cgactctgac tttaaagtct ttgatatcgg tcccgtacag cgcttccgct gctttcttgg
     1441 cagctttttc aacgtcttcg tttgtattca ctgaagccat tgctgatgga atatgtctac
     1501 cagtgactat aaaaaattat tggctgattc aggctggcta accaccaaaa gtcagattat
     1561 agagtctgga caatgacaat ttttctgcag gttctgttgt tgccaactta tcattcacta
     1621 taactgtgta aataacagga tcaatttgga tttttggggt tagagaatta tgtaccatct
     1681 attttttgcc tgtttttctg caactcttca ccggaaacag tttcttttgg agtgccagtt
     1741 tcttcgataa ccggttttta gggctccaga taacaatgtc aggaatttta cctgatttta
     1801 ccgaaccaac ataacctgaa attccgtgcg ctaattccgt ttaacgcaaa gattatcatt
     1861 gcgtacgttt tcattcctta atctacctgt catctttttc gttttatcag cagtctgcca
     1921 tgccattgtg gcaacttcgc ctactctacc cattgcctga ctgcaggaac aatagatgct
     1981 caatgcccca atgtcatgtc ctctgcagcg atagtttcac atctaattct agattcagca
     2041 aatggaacat cttctggcgc agaggaatca aggtggtgac aaaccatcat acccaggcgt
     2101 tctactaaca tattaacgat aaatggccta gtaagattgg tcgaggatgg gaggatattt
     2161 tttttgccgg caattttcat agtatcctgt gcgtgttcac cccagtcttc gttcagtttt
     2221 agccctcaag ctccagctgc aatttgctcg atcaatgcag gttctagcga gtcattcccc
     2281 ttgccaagaa aaccaaagtt aagaggaagt tctaccgtca tcctatggag attccagggt
     2341 ccttgtgtat atgttgttgc tttattgcca ttagcaggac cagtacctcc gccaaccatc
     2401 gtcgttgtgc tgctatagat agcttcaatt actcgttgta aagaaataaa gtgtggatgg
     2461 gtatcgatcg tgtcaggagt agagatggta tgttcgctag ctataactct aaccagttaa
     2521 caaaaaaaaa tgttatagtt atatgcgtgt tcttattctg tgaagtacca aaaaaatgtt
     2581 ttattgtaat gttgataccg cgacatccag cgacttgctg acctatggct atcatattaa
     2641 tataattgca aaaccgatcc tatactgctg gaaatcatga ataacagtac agagcaatta
     2701 tttggtcaag gcgtaaagag gactcaggga gaacaatctc gaagatacaa tttgcttgct
     2761 gccagattga ttgcagatct tgtgaaaaat cttttaggtc cacgaggaac ggaaaaaatg
     2821 tttattgata ttttagggga agttacagtc acaaaggatg gcgcaacgtt attacggaaa
     2881 attgatgtcg aacatccagc tgccaaagtt ctgatagaag catcaaatgc agtagataat
     2941 gaagtaggag acggtacaac atctgtggta gttttggccg gagcacttgt tgagaaagct
     3001 gaggaattac tggatatggg cattgcgccg acgacaatcg tagagggcta tcttaccggg
     3061 ttacaaattt ctctagaaag tctgcaaaat atagcaagaa gattcacgaa ttacaacaga
     3121 gaggtgatac aaggacttgc tactacgtgt ttgagaccaa agatgatatc tcatacttca
     3181 ttcgaagaaa acatggctgc aaaactggtg gtagacgccc tatatgcagt cacggatttt
     3241 gcaaacaata cggttgaagc taataacata aaaatagaag aaaaaattgg taacacttca
     3301 gacacccggc ttgtgaatgg tgttgtaatt gacaaaacaa tagataactc tgcaatgccc
     3361 cggtcagttg agaatgcaag gattctcctg gtagacgaag aattagaaag taaacgaacg
     3421 aggaccgacg ccgagatcaa tattacttcg ccatatcaaa ttcaatcata tcttgacaag
     3481 gagatccaca tgacgagctt aaaagttcag cgtattatta gttctggagc tactgttgta
     3541 atttcacaaa aaggaatcaa cgcttctgca cagaactgtc tatctaaagc aggtataatt
     3601 tcgatcaggc gggtaaagga aaatgatatg ctctggctta aaaaggcgac cagtgccgag
     3661 gtgatcagag acctcagcag tgacgttatc ctccctgaat accttgggta tgctgagaga
     3721 gtctacgaaa aatttgtagg aggtgataaa atggtgtttg tcgagggttg caaaaacccc
     3781 aaagctatga ccatcctact cagagcaggt tcaaagaggt tacttgatga atatcaccgc
     3841 tctgtattag atgcaattgc agtcatcagg aattttatag ttaagccaac tattgtgggc
     3901 ggcggcggat ctgcagaaat gcttattgcg accaatgtta gaaaaaaagc cagttctatt
     3961 agcgggcgag agcaaattgt acttcagaaa tttgctgcag cactagaaga aataccgctg
     4021 actattgcca gaaatgcagg tatgaatacg gttgataccc tcgctcagtt gagatcaaaa
     4081 caaaaacaga attcttcaac ctccaaaaac aaaatcgtcc gctggattgg gatcgacgca
     4141 attggaagga aggtggcaga aatttattct caacgtgtca tagaaccatc tattgtcaag
     4201 gaacagatca tcaaaacagc tgtcgaagtg actactctgt tgattcgcgt cgacgatgtg
     4261 ctaatggcta aacctgctat gtatactcac actcacgcaa acgggactca gcattcacat
     4321 gcaggaggcg aaggaaaaca tgatcaccac tttgacaggt tagggaagca acaacgacca
     4381 gcgcatcact tctactaaga ggaatggaag ggaggacacg agatagaatt attcagttct
     4441 cgctgtaaca cggcccagat gctcatgtag ttgaccttgt ttatgccgaa ttgagactac
     4501 acgtttttat agtttttatt ggcaataata tcctgaaagc gaactaatcc gtccctctta
     4561 tcgctacact gccattgtgt agcggatagt tggaataggc tcgtgaaaag ataatttctt
     4621 ttttggaaaa ttatcaagaa ttacttcgaa ggaaattctt cttagggttt cgggtactcc
     4681 tatcatgact ttatcaggta atagtatctt tgctgcgttg ttctcaatat tttcaattga
     4741 tacgcgagca cgcaattggt caacaacata cccacaataa agtgctagcg attcgtttgt
     4801 gttaattctc aaattctttg caagtttatc tttggttatt tctagtgaat caaaaaatat
     4861 ttgttcatcg aatttatcgc ggtattcaaa gactctagta aaaggaggga catctgattc
     4921 tcccttcacc tctgctttaa ttaaaatcat cgccttctct ttggcgcctt agctctcggc
     4981 ttgccaaagt cataagtatg tcctgtacct ctatagagct ttggtcttac tggattgccc
     5041 tccgtctcgc ctttctttgt tagtgccaat cctgcgagaa gctctgtaat tagcctgttg
     5101 gctaatccgg acgtaattcc accgttgtac gagttttgat cactcacatc gtaatttggc
     5161 gcgacttcta ctaggtcgaa cgcaaacctg tctggatcaa atgaggttga taacaaacgc
     5221 atcaatctca ttccttcccg cgatgttaat ccgtttggtt ctggttcccc tgttcccggc
     5281 gcatatgccg gatcaaatgt atcaatgtcc cagctgatgt agactgcatc aacattatca
     5341 ttggccctat cagctgcctc ctttgcgatt tggtcaattc ctaactcttc cacatctaac
     5401 atggtaaacc attggaagtc ttggtctgcc atccatttgt aaaggtctgg tccaggccag
     5461 tatccatgcg gaccgattag agtatagttc ttccctttta ggcagcctag ttccataatt
     5521 ctcctgatgt gcgctccatg gtcgtacttg aaaccgcaca gtccttgcgg ggcacagtca
     5581 gcatgggtgt cgatatgaat catcccaata ttcttgtact ttttggcaaa cgctctaacg
     5641 tttgcaaatg tgactgaatg gtcaccaccg agcatgactg gaagtccgtc gatatcaagt
     5701 acttccttta ccttgtcagt catccttttg agtgactcta cgacatctcc aggtgagaca
     5761 actacatcac catagtctgc agttttgaaa accccgaaag gatccatgcc tgtctcaata
     5821 ttgaagtgct catacggcgg ggaaggtact gtcgaagcag cacgaatcgc tcttggacca
     5881 tatcttgcac caggacgtat tgtagtacca aagtcaaacg gttctccgat gatagctacg
     5941 tcgggcctaa ctttcttcaa ttcttcatga cttctaagcc aaggtaattt caaaaatgtg
     6001 ggtatgcccc caaatatagg ttcatcattt cctttgtatg attcaccata gaactctgct
     6061 cccatgtctt aatcgctatc ttatagttga taactatttc ttaagcatat tgatcgaacg
     6121 atggtgcatc tgaaatttga tataaatgcc tcttcatcgg gatattagta ctgtcggtca
     6181 agatcttcta ttaaaaattt aaataactag caaaagactg aaaaacgtgg atcttacagt
     6241 tgtatatggc atatttgctg ccattttagt tgcaagtggc gttttattgt accttggcac
     6301 gataggatta aagggagaca aaatggatga tcagttgtat aaagaaggcc aagaacctcc
     6361 gaaatgagtg ccggatcttt tgtctgtagc gtttatttta tcaagtgatt tagaattatg
     6421 gtatttggtt ggttttcaaa gaaagaacaa gataatagta caataaagca caaggtggtt
     6481 agtaggaaag aagcgtctga tgagtatgtg aaatatggtg aggacatggc gaattcaaac
     6541 aaccttgata aagcaattga atgttttgac aaggctatag gcataaatcc taaaaatgac
     6601 tttgcatggg gagacagagg gttgatttta gataagcaag ggaaaacaga agaagctcta
     6661 gtatcttttt caaaagcaat cgaaatcgac ccaaaaaacg cgatcacatg gcataacaaa
     6721 ggattgagcc ttattaggtc taaccagctg aacgaagcaa ttgaatgctt tgacaaggcc
     6781 atagaatgta gagaaaatta tgccaaagcc tggtacaaca agggtagggc tttatctatg
     6841 ctcggagagc tcaagaaatc acaggattgc tttgacaaag caagaaaatt agatccactt
     6901 ctttatacaa agttgaagaa aataaagtaa attactgaag caaattttga aagctatcta
     6961 aatccaacat taatgccatt tctataacag ccaggatata tgcaattgnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggata
     7201 tatgcaattg ttaaatatat aaatataaat attgttaaat aaaaaaattc cactaaatca
     7261 gaggtagaat tgttcggcaa aagaaaatat ggatgtggaa gatgtgggag ttcgttcaaa
     7321 gatcgagatg aactcttgag acatgcagac gaaatgcaca ataaaaatac gacttattta
     7381 tgcataacat gtgacgagga atttaacaac gagtccgcct ttagattgca catggcaaga
     7441 gaccatagaa tataaataca cgagagcttc cggggagtct acccatcatc taccgataga
     7501 accactggag ctagatgtct cttttgttta ttggccatga cagtcagttc gtacgagcgt
     7561 cccacattgc gagacagttg tcggtaatta cattgagatg gcgagaaggc gagtaacatc
     7621 atacttatat aataaagcat taactatacg gcttatcgtg actagcattc ctattgctta
     7681 tcctatagtc gttatgggct ttgtagctgc caccgctgtt ataggcattt tagcaggtaa
     7741 acttgtcaaa aaaagcagca agcggtatat cgtagccggt aagagtttgc cgttattttt
     7801 cataggaacc atgcttgttt cggaagcagt agatgggaat gcatcattag ggaacgtgtc
     7861 agcgacattt aatggcggat tttgggcagg aggcacactt gatcttggat tggcgatctg
     7921 tcttgtctta acaggagcct tttttggtaa aatatttaac agaatgaaca tgattacact
     7981 ggcggatttt tactttagaa ggtatggaaa cgttacggaa atcatgtctg gaaccctaat
     8041 gtctataagc ttcatagttc tagtcgcagg gaatcttgcc gcgagcggtt acattctatc
     8101 cgtagtacta ggaataccgc taatttatgc gatgttaatt tctaccgcaa tagtattatt
     8161 gtacacatat ttcggtgggc tgttctcttg tgcctatacg gacatattcc atatttatct
     8221 ggcagtagcc ggattctggc taggttttat ttatttcatt ggtccgtggt caccaattcc
     8281 aggaggtctg caaacgatta tcgcaaatgt ccctggaaac ttcatggata tgtctggtct
     8341 attggctgtc accaacccag cgagcggtgc actgcctaat tgggctacaa ttatttctct
     8401 gggaataggg gatattgtgg cactggactt tatggagaga gtatttgcgg ccgacggcgg
     8461 acgaaccgct caaaagggtt gctatatggc agccgggctg actttgctaa ttatgattcc
     8521 agtcacgatg attggcatta ttgggcatac actagaaccc aagatcagcg atccctatac
     8581 actcttcccg atagtcgcaa taacccacac tcctgcgata ataggtatta tgatgctcgt
     8641 gagcgtagta agttgcggca tgtcaactgc aaacggtggg actctggcta ttgctagtgt
     8701 tttgtctaga aacatgctcc aacgaaatat tattaagcat attcttaaac ggcccggtct
     8761 ttctgaccgg cagttattga tagcaacaag gctctttgtt atacccatgt tcttcactgc
     8821 tttcgcgctt ggagctatta ttccaagacc aggggggtat ttggtacttg catttgacat
     8881 tgtattggcc ggttgtctcg tacctttggt tttcggaaca ttctggaaaa aatctactgc
     8941 tgctggagca atagcctcaa ttgctgtcgg atcggctctg aggatgatct tctatttctt
     9001 gataagtatt gcaccttcta gcagcccata cgccgcttat gctggcttag atacaatgat
     9061 cccgccacta gtgtcgctgg ctatgtttgt aggggtaagt ctaaaaacac aaaagctgac
     9121 tcctccaaga cacgatgtta tttatctgat tccaagtgac atggatacgg ttacgggagc
     9181 tgatgtgtca gagtgggtcc agcctattga tgtaaggcaa gttaaaaaaa gctgacaaca
     9241 acgatggcag cggaagttaa actagaaacg gccgagaaca gaaaaaataa ctttgttcat
     9301 taaggtcacc aagtttttga agtatacgcc tagctattga caaaccaatg gttagactgg
     9361 aatcgtcgga gttctactga tgatttaaat ttcgtactgc gacattcttg tcttgtgatc
     9421 ataatattag aatagctcct ctgactagat catttctgta tttgaactat gttgatgaaa
     9481 ttatttctgt attaaaaaaa agtgtttttg cagatggata tttacttcat agccaaatcg
     9541 aaaatgcgaa aatggctgat ataactaaga caattgctta tcttcaacaa aaaaaaataa
     9601 ttcgtgttga aggccaccgc aagaactcgc gaacaggact aagcattcca atatactctt
     9661 tagcgtcaga acattcgtcc cttaacacaa ctcacagagg tatcgacaga ttagatcttg
     9721 atgagttact tgccggcgtt ctatcggaga gagtagttga gtatagtttt ctcgcgagaa
     9781 atttactacc gtctagaagc aacatgaaga tactcgatgt tggatcagaa cagtctgctc
     9841 tgacaaaggc actcagcaaa tgcgggaaca ataaaaattg ggaggtgttt ggcatcgacg
     9901 tcgccagatg gctaccgcat gcatttaaag aagagaaaga tgagtcatgt catctattta
     9961 tgagaatgga tgccaggttc atcggctttc gcgacgaggt gtttgataaa ataatttgta
    10021 tcagcaccgt tgagcatatt ggaattcagt caagccatta tatcgtcgga gagtacgacg
    10081 tgctaggtga cgtaaaggcg ctttcggaga ttttccgtat cttaaagaag ggcggtagag
    10141 tcgttttaac aataccttac atcgacagga gtattcgcgg aaaccataaa gagcacagaa
    10201 tttataactc ttatactctc tccaaattga taagtcgctt ccgagtaaag agaaaggaat
    10261 tctatattta catagaaggc aaatggaaaa gttgcaagct gaagaatatt gtaaacaaat
    10321 tgcaactaat cggtaattca gaaatacctt catatttgca tagccgtgta tgtctgtgcc
    10381 ttctgctaga aaaataatac tatcaaattc agccagattt gctgaatcat ttaatgtgac
    10441 aaagacaaca aaaacggaca agttacaaga tcactgaagt acgaagagta tcatttaatt
    10501 aatagaaata ttctcatcgg ttttacaggt gcgtctctaa ccagcgcagt aactagttcg
    10561 acaatagcaa gatttacttc tcgattggtt aattttttaa tgtccttcat tgctgatgtt
    10621 agcgtatttc ttacgatttt tggcgttcta ttctatatca acaacaaaga aaagtaagtc
    10681 aacgaatcaa cccacaagac agattttgcc aagagaaagt gggtctcagt aaagctgata
    10741 tcaacacctt cgatagctga aattaaatat aatatcgtga agcattatat tcacgattgg
    10801 ctgctaagac tcgacaacga aaacagggtc attagtatac aagaaatctc attatgtagg
    10861 aaaagaaaag ttggctcgcc cgttgtcctc atttgctgag cgtaacgatg tgtaacctta
    10921 cgaaatcata agtcgatcat atgacaatac gaagcacgga caggaagata aggaaaacct
    10981 tacaccacga gtatctctcc aaagcaaaaa ttttttggcc acgagagata tatgcagttt
    11041 agtcgatgca ctttggcttg agcgctctta tctgagcact acctatctgc cactcttata
    11101 gtagcagaat tgaatagcct gtgcgacaag aagggatcca gcaaaatgtt cttcacgtca
    11161 gtgttggcct gatatgaaca agaattgtgc ttatccaagt cgtaagataa tacgaaaata
    11221 aatttaaatc aaaacggtaa gagttatcat tggtttcaag aggtcatctt caatttggct
    11281 ataccttttt aatattataa cctaagtcac atctaatata gcaagctgat aggatatgga
    11341 tgcacttgaa attattttca aacttcaaaa agaattggcg cagattacca cttcgacacg
    11401 ctacccgccg gtaaaagagg aacgaatatc tttacttgca actgcaatgg tccacgaggc
    11461 tatagagctt caaagactta ccaactggaa atggtggaag aagcctacta aatttgatca
    11521 gactcgagca atagaggagg tgatcgacct atggcatttc cttgttcaaa catcaatcga
    11581 acttggtatg acaccaaagg aaattctaga tgaatatttg aagaaaaacc aaataaacaa
    11641 ggaaaggcaa gaaaaaggtt actaataaaa tatttgcgga ataataccat cgaaatcata
    11701 aagccaacga attttagatc gaatgcacat attctatata taaaccaaac gagaaattgc
    11761 caattttatt aaattttact caaattgtga tttcttgttc taaaatcttg ttactagtta
    11821 atttttattc cagcaaagtg actttatcaa catggcgaac tccaaggagg attacagttt
    11881 tgggggtaag tatcctaaag atctatttac attattttaa aatatactaa actaatcata
    11941 acttcttatt ttccttcagt aaacgaaata caacttttat actctaattg ttaagtaaag
    12001 ttaggataag ccgtgagcga actaaaccaa taccctcttt tcatctatgt ttgaaaccat
    12061 aacgtcggga tacaacatca ggtcaatctc tcccaacata taactgtcaa gcacctttat
    12121 taaagcccct aattccttct cacgctcaaa atcgttcttt ttccttactt tttttaactc
    12181 ttccatcaat aatcccttaa gttcacctgc atcttctctt tcaagtctac ccagacttaa
    12241 caagtgtaat aaatagcgta atttttcacg ctgggtgtcc ttatcgatat taatactact
    12301 catctttatc ttgtcctact ttttcttctt ctcttcttgc tgctatatac gtatgcagct
    12361 taatagagat atctgtatag gctttccgca tatcctttac gtcatcctta aattctttct
    12421 ccaagtcctt cacgtcagcc ttcatgtctc tctctaagtc tttcacatca gtctttaatt
    12481 ggtcgagttt ctcacggctc cgacctaacg aaaccatcgc cacaccaaat aacccaaata
    12541 tagttgctat accagcaact gctgcaataa taatttcagt tgttgacaga ttcatttcaa
    12601 acagacacct ttcttatgct acgtctgatt tgggtcagcg tgacgggttt ccatatgctc
    12661 tcgaatatgt tctatatatt cattgcaatc tagacagaag tcccgaccat ccttttttat
    12721 tatcctagcc atacctaact gttagtcatt ttaatatatt aggaatacca gattttttaa
    12781 aaaccataca atcgcaacct ttggctaagc agaagtcttt tccgaaacga tgtatgctcg
    12841 ctttatggga gcaatttgga tcattacaga caggatctat agtgctagtc atttcttaag
    12901 tcttgctcta tctttgatat acgtgattcg tgattatcta gtcttttgtc ctctatactt
    12961 tgtttagcat agcggcggcg ttgtctacca tggtatacat ggcatagata acctccacta
    13021 gatgccttat gctttctctg cagcctctag atcgtctatc catgtcaagt acaatccttc
    13081 ataaaccttt atttagtggc cttattctgt tagtacagtt agctaaatga tactcacact
    13141 tcgtgatgag cttgaacaca aaaaggttat acagttagaa tgtcattttg acaagtgtac
    13201 aatatgacat gtaaggaggg ctttaacaaa tgcaaaccaa tagaaaactg ataacagttt
    13261 ttgttcttgc gttaatggcg tttatggtta gtggttctac gatgacaatg aatgtttatg
    13321 ccatccaaac tttgccaact tgcacagatc caacaggaca aaatcttcca tgcattatgt
    13381 tcatatcgac actaccgcca cctaaacata cagtccaatg tcaggaaact tctggtcaga
    13441 tttttaaatg cactttcatt gttgacaaat taagtaacgg aaacaaaatt gtagcaataa
    13501 cagtatatgt ccctgctaac tttgagttca caagtccaac agtgataaaa gtcgtagtgc
    13561 atgagacagt gacaaagaca aaaatagtac atgtaactga gtgccctcca acccagatat
    13621 taaatcctca aactcataaa tgtgtagaca agattgtatg tgaacgtgcc gatagtagtg
    13681 ccataacaga tcttgacaaa aatctcaaaa ctaaggactg cgacataggc gatgcaaatg
    13741 aacatggtat gacaccgcca ccaccaccac caacaaagac ac
//
LOCUS       DAOR01000179            2893 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_104794, whole genome shotgun sequence.
ACCESSION   DAOR01000179 DAOR01000000
VERSION     DAOR01000179.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_104794"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    802..1018
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          914 a          489 c          567 g          706 t
ORIGIN      
        1 cagtttggca cattcaagca agcgtaccga gtccaacaag cagaattagg acttaactag
       61 agatatttgt acctttaacc tgcataactt gcatattctt aatcaatcag ttaagctagg
      121 ctcaactaaa caacctattg gaaaacttgg ccgcaacaga tgcaccggag tttaaagatt
      181 tttattctgc cgcttatttt ctacgaacgg ctttcaaaga cagtaaaatg agtcaaaact
      241 ttatatttcc aaaaccgaaa gatgcagata ccttcgagga tattgtcagg gatgtgtttg
      301 caagggtata caaaaacaat aacctgcaaa ggtatgggag aagcggacaa aaacagcatg
      361 gggtagatat tgcaggtcta tcagaaagta aactgattgg tatacaaagt aaaaaccatc
      421 cctttggaag gataagtaaa acagaaattg ctgaggaaat aactaggtca gaaaatttca
      481 aaccaggatt ggatgaatac attattgcta ccagcgctga tagagacgtg gatattcata
      541 gctttgtact ggaaatatcc caaagcagaa tagcgcaaca caagtatcct gttgtaataa
      601 agttttggga tgacatttac gacctattgt gtgattatcc ggatcttgtc tataagcact
      661 ttaccaaata cttccccctc tcagacctgg aaaatataca ctatgatctg cacaaattag
      721 ccaaaaaaac cctccgctgg cttgtaagcg aagaagaact catcgcgctt gcaaacgaaa
      781 atgtcggggg tattccaatt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     1021 tcaagatccc tataacctgt ctataggatt aaccacattt aaggacgtat catttgccaa
     1081 aatggttgat ctggaattgc agtttagcca ctatatggta aacaacacga atcctctgga
     1141 aagctctcaa gcgcttgctg cttctttgaa gcaattacgc gcaattttag caaatccatt
     1201 tttctcaaaa gagatcttcg tttatctaca gtcaaggtta acagtaagct atcttttggg
     1261 gtggactttc cgtaaggtta caaaatatga gttgaaactc tactccggag atcaattatg
     1321 ggcaacgaac ggattgccgt gggtaagcgc aaagctgtat gatgatttgc cagagatact
     1381 tgaacaagat agtgatgagg tagttgtaat cctgaacata agaaggaata ttagggagtc
     1441 ggtttatgaa tatgtcaagt cgatgccgaa gcaaccaaaa gcagtattga gctatagctt
     1501 gcagggatat acgattgaca gtgccgcaca tgcattgtct ctttcaatag agatcagcaa
     1561 caagatacga aatttagtag acgtatggag agtgaaaaag attcacttat ttggcgcttt
     1621 gccagcagca ttagccaccc tcattgggta tcacttaaac gctatatgcc caatcgcaat
     1681 ttactttttg gatgagtcaa gaaatcagta tcttttaggc gcagaaatta agaatagttg
     1741 aaggagcgtt atggcagttt taaacgatct atttgataca tttttgcaca atattgaacc
     1801 tgacgacgag gctgtcagct atgcacaaga tgcccaccag ccgatacggg aacacttgga
     1861 gcaagacgac caatttaaat cctactttga ggacaccttc ctctatggat catatagacg
     1921 gcatacggca attggtacga ttaaagatat tgatattgtc gtactgacta attttgacac
     1981 taaagctcca gataatacgc caagtgcagt gctccgaaaa ctgaaagctg cattaaagag
     2041 atattataaa gatcctgaaa atcaagagta tcaacgaagg tctatacgaa ttaatcaacc
     2101 gcttccgaag aataaagatg tcgaaatgac attagatata attcctgctg tgccagtaac
     2161 agatgatgat ggtattctgc tcgtgccgga tagagagtca caaagatgga tacaaacaaa
     2221 ccccaagggc cacattaaat acgtcaccaa gttaaatgat gaagatcatt cacaaggaaa
     2281 atttgttccg ttagcgaaaa tgatgaaatg gtggtggaag tatcaatgca gtattgtcca
     2341 gcctgataaa gaaagaccaa aaccgaaagg attttggata gagtgtttga cagcagaatt
     2401 gttcgacctg gcacaggaag atatggcgag tcattttatt cggctacttt ccaacatcag
     2461 tgaggcatat aaaactacag atggagtgcc ggaactggat gatccgggcc taaaaggaga
     2521 aaaaataaaa gcaaacataa caaaagctga tttccagatc tttatgaaga caataaacgc
     2581 tagccttata ttgacgaaac aagcgtatgt ggaagtggac gaagaaaaga gttcaaaatt
     2641 atggcgacaa gttttcggtg aggtatttcc tgtttatgag ctagcggaga gtacaaaagt
     2701 ctttcaggaa tcagtttatc ctttaggggg agtgtcccac gccgatacaa taccatggcg
     2761 ggaaaatctg cggtataacg ttaatgtaga tgcgtatttg tactccattg ataaaaagat
     2821 aaaattccgt gggattaact cagatgccag atttaaagat aacttacaaa ttattgacgt
     2881 ttagacaaaa gta
//
LOCUS       DAOR01000180            6363 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_40171, whole genome shotgun sequence.
ACCESSION   DAOR01000180 DAOR01000000
VERSION     DAOR01000180.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_40171"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    666..865
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1833 a         1194 c         1265 g         1871 t
ORIGIN      
        1 agatgtgtat aagagacagg cggatgccac cattatggat gtcatgcagt tcagtattag
       61 tatattttga agaataatat tgttattttc tacagtaaaa tgtataaatc gaattttggg
      121 aaaatatgct aactcacagg taatgcttct gcaaactcta taaagcatca tcaaattcaa
      181 tcatcaaaag tgaaacattt tgacatattc tgaaagttat atgcgaaaag ctacgcaacg
      241 ataggtagcg atcgtagttg gggggttcct aatagaagat cgccgccggc caatactacc
      301 ccgtttttta tacaatcacg ggtgcgtgcg ctcattctat agtgcttatt cgcgcatcgc
      361 gtggtgaata ggttcagaaa gaagaaggca acctaagtac tcgtcacttt ttccccctct
      421 ccgcctatcg tgatgagcgg tactctactt acatatatcc aaatcaacag ctcttttctg
      481 ctgcagtatt attacttatt caatgtaaaa ttagcattct tatatgtggt tatgaaatat
      541 ctttggtcac atcaagagaa agttattttc ggaatgcgaa tgtggggaaa atgaattttg
      601 ggaatatgaa taatacttga gggatgattt tggccgctgc ctgaacttgt agatgaactt
      661 gtagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnngaact tgtagatgaa cttgtagatc tgtgaacact
      901 atgatgagct gggtgactac tccccgtgga gctagtctta tgaacagttg cggaattcac
      961 gttaaatgtc atcgtcgtag gtgttatttg attctgatat ccatttgcag atccgtacac
     1021 gtcgacggtg aatgtacctg gtttgtaatc tttgcctatt ttccagctat atgaaagaat
     1081 tcctgacttg ctggtagttc cattaaaatt cgttacagta gtgttggcgg aatcagtaac
     1141 ggttcccttc acgcttgctc cagctaccgt ggcgtttgag tttgaggcat ctaggactgt
     1201 tgtcttaatt gtctgattac ccccgctgtt aatcggattc tttgctagat cgagtgaaat
     1261 taataatagc tttgatctat tattattgat attgttaaga tttgctgttg aaccatgtga
     1321 tgagacgggt gcaacaatag cagtattgct attagctact ccagttacat tgacactgtt
     1381 atgtaatgtg agactagggt tattactaca ggagaacttg gcagttatct tattttgtcc
     1441 atccttaata gcagaatacg taggcgtaag catataattc catgttgagt aatcatttgc
     1501 tccacggccc cccgttggga caacttgttg atatggcttg atgccattta cgataactga
     1561 aactttgcaa tcagcagagg ttgttccatt atcaatggca gtgcccgcta tggtgaggtt
     1621 gctgcctgcc ggaatttgtt gccctttggt aggtgacata atttttatca catgtagcgt
     1681 ggtctgaact gaagaaggca gaggtgatga tggtaacatt tttgctccac taccattatt
     1741 gcttgtttct gattttggta ataattgtgc atatgctgtc gttgtgattg tatgtgcgaa
     1801 catggtcgaa agaagaagaa tgaccgtacc aaaagaaaaa agcggtagca agtaaaagtg
     1861 tgatacaaac tttgttggga tcatacgcaa tgatactaac taggtgtgat attataaaaa
     1921 cagtttgaag actatattgt atcagatagt ttagcgagtt ttttgcagta gaatgaacag
     1981 gaaataaatt ctcttcagta actacctcaa aggtcgggtt gttgttgtgc ttgacaagat
     2041 cttacctcgc gtctgatctc ttagacttga cacttcttac tttagttaat tcaataaaag
     2101 gagaggagaa taataggatt aataattgaa taacagaacc taattgattc cgtgttcgtc
     2161 cgcttcaata actaataggg tcttgctaag atatattatt attatctact tttagaatta
     2221 gtatcagaac aaaattctac catgatcgaa actacgacaa ctgctcttgc gaccgcttcc
     2281 tcagctgttg gaggaaacgg ggaaatatca cttacttctg agtatgtaat cccaatattc
     2341 ttgaccatga tatttgctgc tgccctattt tcttctagaa cgaaagtgcc tcatacgatt
     2401 gtcctcgtgg gatttggaat catcatttca tttcttagct ttgctgggat agagatagta
     2461 aatattcaac acttcagaat cgatccgaat ctattaatcg atttcgtaat accaccatta
     2521 atttttgaag caatgatgaa tgtggactac aaaaaattca aagttgtcag aatatctgca
     2581 ttattactcg ctactgtagg tgtggttctt gctactttag ccggtggatt tattatgacg
     2641 tatatagcag gtttaccgct tcttgtcgcc tttgcctttg cagccttgat tgctcctacc
     2701 gatgcggcaa tcgtaattga gatattcaaa agggtaaggg tcccaaagat actctctacg
     2761 ctaatggaat ctgaggctgc cttcaatgac gctactggag ctattgtttt ttcctctata
     2821 attgcaatag cttttgccag ttccggtagc gcaattcttg gttctgctac tccctctttt
     2881 tcttcttctg tgctagggaa tatcgcgatt acagccccca acgcctcgat taatttagcc
     2941 attgctaatg gagctataca ttttatagta gtattttttg gtggtgcggg tattggattg
     3001 gcaattgctg cggcaacgca cagactccat ccactaatga acgatccttt ctcagagact
     3061 tcactaacta ttgttaccgt atttggttcg gttgtacttg caaactcgtt aggtgtatca
     3121 ggtttaatgg ctgtagcagt cgccggacta tactttggca atgtgacggt gaaaaaagag
     3181 gttaccatga gtaagaacgt tagagccttt gccttcactt tctgggaaat gactgcattt
     3241 tttgcaagtt ctgcagcgtt cctttaccta gggattagca tgaatattgt tgatgtagtt
     3301 aaacacctcc caataattgt ccttgcattt cttgcggtgt tagcagctag agcagcagct
     3361 atctatccta tgcttgccgt aacgactacg ttcataggag aaattattcc aatgacatgg
     3421 agacatgtta tactgcttgg tggaatgcgg ggcgcaatat ctgtggctct agtcgcatca
     3481 cttccaccaa gcaattttaa aagtatattg cagactctaa cattcggtgt cgttctgttg
     3541 tctttggtca ttcaatacgt agcgctctca aaatacgtga gaaatgtttt tcatcatgac
     3601 gatgctagtg tcgacaagca acaaacttac tgagtatctc tctccgaagg atgcatggga
     3661 tctaagaatg cgttaccccg cttccatttc gttgtcgccc cgtattcgaa gcaatgaaaa
     3721 cacatgaaaa taattgactg caatatataa tgtcaactta caatcatgca ttcgagggtg
     3781 agctccgtca taccatgcgg agtcaaagtt tatgtcgata aggttcaggc tattttttga
     3841 agagggggcg atggctgcag gataaccaca ggtaagtggg aagtatcaaa acacggaaaa
     3901 tatggataac aagatacagt tttgccgcta gatacataac tggaatagcc aataaatgcc
     3961 tccgtttaag ggatttggcc gcatcatttg taagaatata atacatgttg gttgctgcta
     4021 cctagatgca tcaggatctc tctaaattct atacgttttg atctagggtt tgtaattatc
     4081 ccttgaatca actcaaggtg ttacaacggt tatagggtcg actacgacgt caagtagaag
     4141 ttacgagatg ctcgaaggat agatggcata aaattgttaa gcccacatag tgctctaaac
     4201 actggatact tgttattgat aaatgagaaa attcattcca attatataaa tgagaaaaaa
     4261 cgcttcacga tgatggcagt agtactcatt gttgggatca tacttggcac tatatctgtg
     4321 actacgctgt ctcctcaatt gagttatgca caaaatgcga gccgtggagc tagtgtggta
     4381 aataatcaaa cgactgcggg taaagtcgca tctgctgcta cggcaacaac acccagcaac
     4441 tgtactattg gtttgaagtt gggtaatata tcatacccac tcaagtatca aatcactggg
     4501 gggcaatctc gcgagtatct ctgcagagag ggataacacc acgcttctgg tcaccgtctc
     4561 gtctacatcg aatggaaact tgactattga acttccacga aatgtaatag actctaaaaa
     4621 acaagggaac caagacgcta atttggtggt gtttcaagat ggtcggtata gttcagcatc
     4681 agaccaaatc aaaaccgacg cacaggtcag ggtattgtcc attagttttg acattggtac
     4741 tgaacatata gaaattgctg ctcactcata tgggtccaac aattacacca caagtaacaa
     4801 ctaccactcc aactacaata aacccagcta atgttactgc tgctccggca tctcaagtaa
     4861 cgaatgctac caaagctcca gctaatgcta ctaaagctac gaatgcaaca tcggcaaaat
     4921 cttcaattaa cgctacaaca gcagcaacga caacggcttc aagcaattcc actaccagcc
     4981 taaagagtgg tagtaaaatg tatcctctta attatcgcat aactggtggt ggtaatatgc
     5041 tcaatggtgt ttctttggag aaagacagct ctacgctgct gctaagtata tcttcactct
     5101 cacatggaac actaacaata gaacttccta gaaatattat tgactctaaa agacagggga
     5161 ctaaccatga cgaaaattat gtagtctttg aggatggtca atatgtccca gttacccaac
     5221 taaagaacaa tacccaatta agaacattag caattgattt tgacaagggt tctttacagg
     5281 tcgaaatagc tggtactcat gcggtgccag aatttggaac agttatttcg gcaacaattc
     5341 ttgccatcgc gatcattgga ataatcgttg ccactacaaa attcaatggg ttatttagta
     5401 tcgtgcaaaa gcggtagagc aatcttcttt cgttcctatt tttctggaac aagtcatcga
     5461 tatttatttt ttgatttaga tgatcttttt ttctgtctat tcaatttcag tattattatt
     5521 acggcaacac cccataatgt gagtgctaaa agtctctgtt gcaagaacca cgcaatacct
     5581 cctgctatgg caaagatcat ccccaaaatc ccaataggtt gtagtcgtct gaccaatgtg
     5641 gttaccccag attagatatg gaaggatcaa taataagaat acgactagaa gatgaaacat
     5701 tggaagctac tataaaaaca atccctatat agctctacaa aagcatagtg tgaatacatg
     5761 ctcatgagca ataaatacgc tgagaaactt cagaagataa tctcaaggta gatttacgat
     5821 aatccattcg gacattaccc ctgattagtc caagtacaca agaaatttag tcaaaagagc
     5881 attattttct gcatcaggag cggcctttct cgttatttat taccacatca acactatcct
     5941 acatgcatct tcgcctttgg gcaaatgata ctgcaaggca gaatggaatt cctcaagtgg
     6001 caactgggac taataaaagt aattctgatg ccgctatgaa tagtacccag caaataaatt
     6061 ctaacaagcc taaaaaatgg ggctcaatac cacgagtcat gacaaagatt tcagataaag
     6121 gaatctatga acacaaaagg aatcaactat tagaggtgag tcgagtctga gcgcgagtcg
     6181 gttcagcgtg ctatctagaa tgtaatgatt actgccaata gattgttttg atatgacaat
     6241 ttactccgag agtggtaaac aaggcaaatg agcctgtaac ttgctggaag agcaactgag
     6301 gcaatttcat tcaatattag ctgtagcact tacatagata ggtgacactt tcaaataccc
     6361 aca
//
LOCUS       DAOR01000181            2930 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_89978, whole genome shotgun sequence.
ACCESSION   DAOR01000181 DAOR01000000
VERSION     DAOR01000181.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_89978"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          997 a          497 c          644 g          792 t
ORIGIN      
        1 caatgaaggc tattgcgtag ctccgtagcc atctatctcg cccaacaact gaaatattag
       61 aaaattggcc tagcctctac ttttttctat ggagatacca cgtcattacc accgcatgga
      121 actatagcac cttaccacat tttagtacca tttcaagatt gatcgcaaga taagttaata
      181 agaacaaaat gagaacttgt tttgatcgta gaatcaatgg ataactctta taatcgttca
      241 aaaagccaac agagaggcag aattgcactc gatttggaac atgaaaatat cttggaagaa
      301 gatgaaagaa gaatagatac taagatatgc attttgtacg gggtagaggt ggaggaggca
      361 aaggagattc atgcaaatta tcattccgat agacttcact ttcttcttcg agatagagca
      421 cggaagcatg gaatgacaga atcaaccttt ttgaagaagc tggaaacctt gaatttttat
      481 tcctatgcat atatggtatc tcgaggacaa ttgatggaaa tggctgcagt cgatatactg
      541 tacaaaggat tatgcatctc agaaaataat ggacctaata aatttgacga tcggtatatt
      601 agcttttatg ggtagaaaat acattaaccg tcaaaccttt tgaacactgt ggggatacgg
      661 tccttcaaac ttcgaagatt agaaaccaac agctagttta tggtctatgc aaggagagga
      721 caatgtctta cgtttgttgt acgttttagg ccaaggtcga gaagacagag cagttgtagt
      781 tacaggaatt accacagtag acgaatatac cagttgcata tttaacaatc aacagacagt
      841 tgcattgact gactaactta cctttgttat attatagagt actatagtta gtattctcac
      901 tatacaactg atatcatata ataacgggga cgtctcttga ttcttttgca ttcgctagga
      961 tttggggaaa gtctgtagta tgtaacatat gccttagaac gccaaaacct ttccaaatcc
     1021 atcaatctgt tttaatgata ttagtccaaa aaatgcaatc gtaccacaca ataattatga
     1081 tgggtaattt tagaatatta gtcggaatat tctaatatgg tctcagtaaa atgacgatgt
     1141 cgtgaaaata agaacaacta atccggctac agaacagatg ctcgaagaat acgaggtgat
     1201 ggatatggac gatataaaca aggcagtact aaaggcaaga caatcattca aagaatggca
     1261 aaccaatata caaaatagag ctgcctttct ctacaatgtt gcacatctct ttagaaaaga
     1321 caaagaacag cttgctaaac ttataactac ggaaatggga aagccgataa aggaagctag
     1381 atcagaagtc gataagtgtg cgtggacgat ggaatactat gcggattatg gaagtaactt
     1441 tattgctgat gaaaccacca acacggacgc tagaaagagc ttcacagcat ttcaaccact
     1501 tggtgtcgtt gggagtgtaa tgccatggaa ttttccatat tggcaagcac taagatttgc
     1561 cagcccatca ttaatggccg gaaacacgat tgtattaaag ccttcaagtg ctacgttaca
     1621 atgtggtatc gaaatagaga aatcttttca gagagcaggc cttccggaag gggtattcca
     1681 aacagttgtt ggtgattcta ccgttgcaga gatgcttata gattcagatg ttaacgcggt
     1741 gacatttacc ggtagtgttg atgctggtaa aaaagtaggc cagcgagcag tggcacaact
     1801 gaaaaaatgt gtactcgagt tagggggaag tgatcctttc atagtttgtg aagacgcaga
     1861 tttagagaaa gcatcgtcgg gtgcagtcaa aggccgcttt attaattgtg gacaaagttg
     1921 tattgctacc aaaaggtttt ttgtcgtaga gaaaatcgca aatgagttca ttgaaaagtt
     1981 tacccaaaaa acggaaaagc ttaaggtagg tgatccgctt tctgatgaca cagacatggg
     2041 acctctagta agctctaaaa gccttgagaa tatagacgaa attgttaggg atgcattaga
     2101 ggctggcgct gaagctctta caggtgggga acgaataaag aataagggtt acttttatag
     2161 tcccacaatt cttacgaaga tatccccaaa gatgcgaata gcacacgagg aaacatttgg
     2221 tcctgtcgcc ccaattacag ttgttaaaga tgaaattcaa gctattgagc aagcaaacaa
     2281 ttcagaattt gggctcggag cgagtatatg gactcaggac cttcataaag ctgagcggct
     2341 gtcaaggcta gtggaatcag gtatagtgac tgtgaacaac gtggtagttt ctgaccctag
     2401 ggttccgttt gggggaatca agaatagcgg gtttggaaga gaattatcta ggtatggaat
     2461 gctagagttt gttaatatca aatcggttag attctatgat caattgattt acaaccacca
     2521 tgtagaataa taagacaaca gtctatagca taaaaataat agtcattttt ggaatagttg
     2581 atagacccac aatgcaaagc catattcgta ttttaatata ggaatcatcg aagaaattta
     2641 cagagattca caataatgtt agcggaccga aaagaatgct ttattgttga atcgatgaaa
     2701 gacgcaaaag gaattcataa gcatagcagc tcgcgagtta cgaaatcctg tccaaccaat
     2761 tcttggcttg acttatctag aataggaatg gagaagcaag aagaaattcc ctgagaaaat
     2821 gacgataagg acgatgatat cttggatgtc attatccgaa atgcgacaag actaaaacat
     2881 ctcacagaac ggttgtagat attgtagaat ctcatgtcgg caggatatgg
//
LOCUS       DAOR01000182            2973 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_55509, whole genome shotgun sequence.
ACCESSION   DAOR01000182 DAOR01000000
VERSION     DAOR01000182.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_55509"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1026 a          493 c          562 g          892 t
ORIGIN      
        1 gtctggaagt acaacagtta accatagctt tcttatcact taaaaatata tatatgtctt
       61 cttgttcata gccatttcgg tatgatattt ccttttcatc tttctagaat cctacaattt
      121 gcatccgtgg gtaactaagg acatcaccag tttttcacat cagcagttac tataagaaat
      181 cgaggttggg cttactttgt tttgattgga aaagtaaaga aaccatgtca aatggctctt
      241 taagatagta gccgaattag tgtttttttt accgggtctg ggtatctcct gcgcggagtt
      301 gcttcaactg actctcagat aggtgtagaa ggaaagtatg tctgttttag atggtgggaa
      361 acatcaggtc atacggctga ccccttctta agaattagct atgacaatgg cagggcattt
      421 gcaagacatt tggagaaaag atcatgttaa gtaatgctac tactgctgtt agtggaggat
      481 aataaattta atataatcag actagcagca catcataaaa acagttaaaa atatagcaga
      541 actctttctt gtgttccaca tttgtgtcta ggttccaaga ctgctcagat atgaggctgt
      601 tgggacaact gctggtgagc ttgacaacta caagtattcc aactacgaat ccctcataat
      661 gtgttgtgaa agaaaaccca tatattttaa attagacatt attaattaat ggacaaagac
      721 ataaggcgtg caaaaattag cttattaact acaggattta tgattgcgac tgcttttctt
      781 gttatgggat ctttacaaaa ccaattaact aaaacagcgt tagcacaaac atcaactaag
      841 cctaccagca cctttagtat gaatggagca ataagttctt tagtactagg aatggcaccg
      901 aacactaaga ctgttgatat gtctactgtg caaaaattca tcctatcagg tgattggagt
      961 atgagcgtaa ataaagggaa cctaacaagt tttgcagcta acttttatac aggccctgtg
     1021 ggtggtggaa ttaccaatca tactcatcaa ttgagtagct ttcgagttaa taatggtaat
     1081 aatgttccta ttcaacttac tccagataga accgtatcta tatcaggtgt tttggatgtt
     1141 ggaacaaatg gcaagaaggc atggaatgat gtacatacaa cagtggttat ctctaaaggt
     1201 agaacaatca ctattagtct agccgataaa gatactcaga gccattttat gggacaacaa
     1261 atctatggaa tcgttaaaag atcaaatata caatagataa aaaattagaa acaactagaa
     1321 tgccacaagt ccagttattt ctaatcttat aaacaatgcc atcaaatcca caatcggagg
     1381 cgaaattatt gttgcaatct gaaaggagtg gtaatgagat ccttgtgagt ctatactacg
     1441 atactgggaa gtgtatagat gctgatattt tgccacaatt attcacaaaa tttgctacaa
     1501 aatcttatac gggcacaggt ttgggtttgt ttatttccaa aaatattgtc gaagctcatg
     1561 atggtaaaat ttgggctgaa aatcatgctg atgataaagg cgcgttcaag tcacttagaa
     1621 actactactg cgtttaaata atgtgaatta gaattatgct gacaactatg tcagaagaag
     1681 gaactgaatc aattaattaa aagttaataa aagttgctaa ctgtgatgaa atacctgttg
     1741 gaaagatact gcgtgcttag cactcttata atgatgatat tgatatttgg acgtgcaatt
     1801 cctctgtgag tcggtactat acgggtatgt aaaagtgcat actatagaga gtgattgtat
     1861 tgatgaagca attctactaa aaatcttagc attatgagac cactacctac agctgcataa
     1921 atcgcctctg aaagaattgc ttgttacggc ttatacgcaa aagcgcgagc agcttggtat
     1981 acgtaaatgg catttttaaa aaatgctatt aacattttcg gaaggacaaa gacaatagga
     2041 gttatttcga tcatatcgat aattgttatt tcatatggtt tattttttta cttacaaaat
     2101 gctactgaaa gcaatatcag aaatagtctt tttgaacagc aaaagcaacg cagctagatt
     2161 ctacaaaagc catctctgag cgtattagct ctgatctcga tcctataatg tcaaggcttc
     2221 agggattagc aaattccgtt tatctacagc aaggagattt atccagtaat aaaacaaaaa
     2281 agttaatgaa agaagactat cttccactaa gtacaacttt tacgactgac agacttctta
     2341 ttttaaacac aaatgacata tctgtagacg cattagccct aaaaggggaa cagatattca
     2401 caggagtgaa tttttcatat agagactggg tgaagcagac aaaaagtaca ctcatgccag
     2461 tattttccaa tggctttgag ggaagagatg gcaaatatag aatagctata acatatccaa
     2521 tagtaaatag ggagacagga aaatatctag gattggtagc agcactaata cctactatcc
     2581 aattctttca acactgtgga aatatttaca atatcaattc gcaatacctt gcagttttag
     2641 accgtcaata agtccaattg attcacccat taaaaacatt cgtgggaact ccattctttg
     2701 gaaagtatgc gcaagaactt actggacaca atatggttct aaataattta atacgaaaag
     2761 taatatcagg caaacccgat tttgccgttt atgattttat aaatggtcaa taaactgtga
     2821 cagcactagg aggagacaac tattaattca atgtggagga tatggaaact tacaaagaat
     2881 tcttgtgctg ctacgacggg ttagttacat tagaattcag aacaaataca tgagttacaa
     2941 agttagtcat ttaaaagaat agttactcat aga
//
LOCUS       DAOR01000183            2688 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_69499, whole genome shotgun sequence.
ACCESSION   DAOR01000183 DAOR01000000
VERSION     DAOR01000183.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_69499"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          839 a          576 c          532 g          741 t
ORIGIN      
        1 cctttgaacc agtttttctt tgccatgcga ccttttatag ctcctactat aaacgaagaa
       61 actacactga ctcctattgc tattgctatt gagacccctg acgaaattaa tccagctttg
      121 acggcaaaat atggcaagat aggaagaatg ccacccagca aaaaagctcc gaacatgacc
      181 agcgcacccc tgaaagggct tcccaatatt tccaagttta tgcctaactc ttctgttaac
      241 atcgtcttga gccagacatc cttattcgag gttattttat cgacgatttt ttggagttcg
      301 tctccttgaa agcctttggc cttgtatata tcaactattt cctgacgctc cttgttaggc
      361 agattttcaa tttcaaactc ttcgcgtctt atttccgatt ctaatatctc tctctgagat
      421 ttgactgcta ggtaattctg gaccgccatc gctttggccc cggtaaacat cccgactaaa
      481 gccgccaaaa tcacaacatc tgccgaaatt tgtgctcctc ccacgccggc aacgatgcct
      541 aaggatgtgt ttatgccatc accaaatccg aagactatat cgcggatata gctgcttgcc
      601 tttatgtggt gctcgatacg cgtaggacct ttaatcaaag tcaatcctgt tattttgtca
      661 tttcaatata tatacgatag cggtgtatag agatggcttc cagccgctac tactggagca
      721 aagacaagaa atgactgtag gtggtaactt acggtttttg atattccaca aacctttggc
      781 cgaggaatct gtttatacaa ttctccgttg cttctgaatt ctttattccg gcaaacccaa
      841 gtactcagcc agtagacaga gaatcctgta actcgattag agtgcgacat attaaggatg
      901 cgtacccaac atactgagaa aggaagtcgg ctgcggattt caatgcaccg ctacctctat
      961 aaccttcgtc atatttcatt tctacaatcc ccttatctgt cttaagtagg accgtgatca
     1021 acgaataacc gcccaagcgt ggatcgcccg atagcacatg ctctagggaa ctctagcttc
     1081 aatgaaagaa gggggccact accagttttt gctgcaacag accatcgtaa cgaacacttt
     1141 gttgtatgct ctgctattgg ccacttgtac ggattggtgg atgataagag aaatcgaagt
     1201 gtgtatcccg tgtttgatgt taggtgggct ccgacagcta caaggtctga acagatcata
     1261 aagtcaatat ccttgctttc ccaaaaagct acaagatatg ttcatgcttg tgattatgat
     1321 caagaaggtc aagtaattgg ttataatatt ctgcaatatg cctgtaataa caaatatgat
     1381 aattcactaa gggccaaatt ttcaactctg acagatgaag agataagaaa ttcatttgat
     1441 aatttgttga aacctagcgc aagcttggcc caagccggga gatcacggca tatgattgat
     1501 tttatttacg gagttaatct ctctagagca ctcacacagt cttttaaggt cagtaatgat
     1561 ggcaaacggt attgtaatct ttcaattgga agggttcagg gcccaactct tgcttttgtg
     1621 gttgacagag agatggaaat cagaaaacat gtccccgaac catattggac tatatctgca
     1681 gaatttgaga aaaatgggca tataattgag gcccattatc accaacaaaa aataggcaca
     1741 ttatctcaag ctacttctat agttgatgca tgcaaaaacc aggacggtaa gattaccaac
     1801 atagagaaac agaaggtcac tctcagggct cccactcctt tcaacctggg tgatctgcag
     1861 aaggaagcct atagagtatt caggttctcc ccaagctata cactatcgat cgcggagaag
     1921 ctatacattg ctgctttgat ttcctatcca aggacatcta gtcaaaaact gcccacttca
     1981 ataaactata agaaagtcat ttcgggcctt tcaaagattg gttatcccta tagtaacctt
     2041 gctgcgactc ttcttgcaag agaccatctc actcaaaatg aaggaagcaa aactgatcca
     2101 gcacatccag cgatttaccc aacaggagaa aaaccgaagg gtaagctagc tggcgttgaa
     2161 cttaaattat tcgatctcat aatcaggaga tttttggcaa cttttggaga tccaaatata
     2221 agtgagcaca caacagtcac gattcttgta aaggactacc ataatttcaa agctgatgga
     2281 aagaaaatgc tttatgaagg atggatgaat ttttacaaac catatatcaa taaatctagc
     2341 aatggaactg aactaccaga acttcatgag ggtgacatga tgagaaatgt tgctgtgact
     2401 atggataaga agtttaccca acccccaccc agattcaatc aagctagttt attggaagaa
     2461 atggaaaaag aaaaaattgg aactaaagcg actaggtctg aaattatcag cacactgttc
     2521 aaaagaaact atattgcgag tactactaat gggaggggag gaattgaggc tacggaaatc
     2581 ggatttcaaa taatacaatc aatgcgtaaa tatgtaccaa gcatagtatc tacagatctt
     2641 actagatcta tggaagaaca gctggaagaa attgaatcag gcaaagcc
//
LOCUS       DAOR01000184           10088 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_27391, whole genome shotgun sequence.
ACCESSION   DAOR01000184 DAOR01000000
VERSION     DAOR01000184.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_27391"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3121 a         1982 c         1945 g         3040 t
ORIGIN      
        1 attgcctttg cactaatgtc gtatccatat gtgtcaaatc ctctctcttt cacatacttt
       61 gccacaggaa gtcctagctg tcctagtcca acaattagta ctttgtcgaa ctgaattgga
      121 gagtatcgtt gtcgttgctc gatcacgctt ttctcgtgtg ttggcatagt caactcattg
      181 ttatcaaaca atcgagtttt tatcgttcta ttgttttata tatgctcaga tgaagaaata
      241 tagtaaagtt atactattta ctggtaacaa ttgatcagct ataccaaaac agtgcctcta
      301 aattattgag aaatagtatg gaatattggg agtactattt ggtgaataaa taatagtgtt
      361 cagctttgta gccaaacgat gtaaaagaaa attcttagta tcacaattct gcatccatat
      421 tctgaagaca aagccttgtt atatttaaag aaaatttaat gctgcgacgt tgatattaga
      481 atgtataact gggtaactca tcaaataaaa acctacagac tgcgaggaaa agatgttaaa
      541 tactactttc tatacggcct atacgtactg atattatgta atggcgatca tagcgataag
      601 caagtcagga tgcaaaataa atctaattct atcatttaca atgcaaatac aaaaataaga
      661 aagtaaactc taacataaaa ccggtaggta catcacgcat tccttatgac gtttcttacg
      721 acagataatc tgaaagtttg atgttttctt ctccaggaat tcttgcaact tcgaatctct
      781 ccttaggttt aaggagagtg gcagtagcat caattcctac cttggtcgta agcaaattct
      841 gctgatcgct cgatgggtca agactagatc ccttcgcatt tgtaactatc ataaatcctt
      901 tatctgcctg acatctagta cagatcgcat attctacagc aataggatcg gctgggttta
      961 tgtcttcgtc taccacaatt cccatcttga gagaagggtg ggaagcaaat gcggccagaa
     1021 tagcattttt tggttcacct tcaagacgct tgcggatttg aattactgca tttaaccagg
     1081 tgcagccccc ttcactaaga tggactgcaa ttgctgtggg aaccacgttt ttaactcctt
     1141 caaatatttt tgcttccaca ggcaaaccca tcaatagtcg atgttccgga tagcctggta
     1201 aaatatcatg caaaatcgca ttatttctga attttatcct atcgagttcg aagaccggct
     1261 gtcgccgctt tatatcatag gttctaagcg cttctaccat ccattcctcc tccgtcctat
     1321 ctgtaaggat ggtgccttct agtacaattt cgctaagagt tggaataaaa agctgggaat
     1381 aattgctcct tgtaaccgta agttttcggt gcagcaatga gtttgcgata cacatttcat
     1441 ttgcaccgta tgctgcctga tatgctgatg caatactaat tgccggatgc acgcctatta
     1501 cgatggagac cttgagatct tgtccatgct ccttggcaaa tgaaaagcac ttgtggaggt
     1561 gtctaccctc aaccattcta attaccagat gtctgtcgtc taacaatagt aggcgatgtg
     1621 tggaagcatt ttggtttccc ctttcctgat cttttgcaaa aacaaccgag gatgttatat
     1681 atgcgcccgc atccttttca aaatgcgtaa cgatgggtag gtctcctaaa ttgttgctgg
     1741 aattctcttc aaaaggggcc ttagtgaaga tttttttcgg ttcggttgtt cgtgtcattg
     1801 ccgctgtgac gtatgaatga attaactctt cctttttcgc cccaatggca agagaaaatc
     1861 tccttcggct acccaaaatg ttacatgcaa ctctgattct gctgtgatta actgtttcaa
     1921 ataatattgc ctgtcccccc tccagcttac tcgcgatggc agcaatttca aattttggct
     1981 caacgcattt ctttattata atgagatcct tttctttttc tagtaaacgg aggaaggcgc
     2041 gtaagtcatt atttttctgt gaagtttcca ttttgtttgt tgtaggtccc aacatcttct
     2101 ttcccaacca aattcataat ctctcccata attgctttca tatcgtcgag atgcaactgc
     2161 tttaagctgt gcatgcaaac aagacaaata ccatttatca tggaagagac cctttccgaa
     2221 attgtattaa tgaacataga atcatacttg ctcggaataa cttttgctgt gttaacttta
     2281 ttagaagagg tcatagaaac atacaaagat ccaatccgat tcgaaccttc agcaactacg
     2341 ataaaacagc cgttctgaaa agataaaaat tttataataa aaattctagt actcgtcgct
     2401 tttaccttgc gtgcgatgaa cgcatcggat ccttcatctt gcattactaa gaatcaatct
     2461 ctatacatga ttctaggtca tttaaaccat ctatttccat tagtttacgt aatttgactg
     2521 ttagaacaac ggctaataga taaaattaag gagtatgcga gctacaactt acactatcac
     2581 taccataatt gcttgaaaaa tctggtccgt tgaggccagt ggacgtgctg acagaactag
     2641 acttgccagg tggagactct caaaccttat ttcaattata aagggattca gaatctgaat
     2701 gttgttacag aatcttatac cgtatcgtat taccaaaaat acgaatgatt ctcatcataa
     2761 tctttttcaa aataccaatt ctattggtaa gggggttggt tcttctgacc cactcacgtc
     2821 tagaagattg ttctaactaa cgagtttatt gcaggttatg gccgattatc aactatcaat
     2881 ggtttttgat gataagacct tgctccaatc gatgcagtag tgatagcagg agttctaata
     2941 cttctgacta attactcctt aagacaaaaa ccttcagaaa ataccaagaa agaaacataa
     3001 accctgtctt agaagtaatg cccgttattg ccacagtgcc attttctgct ttggcaatgg
     3061 cgattccttt tcgaaatcaa gaaccagccg caaatattgc atcttatagg ttcttgtatc
     3121 taattgcgcg gtcggttggc aatacctagg actgacatat gtcaaagaaa gtcactagca
     3181 atccctagct ttatcactag cgattattgt ggtagagcga gaaatctcgt tgtacatcca
     3241 cgtattatct gtggtgtgtg ttccttaggt cgctgcccat catcgtctga ttcgtcattc
     3301 taacgggagc acaaattgaa tgacggctga atacttctct cttattgctt tcatcgaatg
     3361 tccgttttct tgtttttctt ctccttgcct tccacgtatt ctttcaatga cttcaaatta
     3421 tagtcataca tatcctcaaa aaattctact aactccaacg ttctatgtct attcaatgaa
     3481 taaaacctgt tctttccctg ttttttctcc gttatcaaat ctgcgtcttt caatatacgt
     3541 aggtgagatg agagagctgg caaactaaag ttaaagtgtt cagctatctc agtaggtatc
     3601 atatctctct tcttgagcaa aagcagcatt tctcttctac tgtcatctga tagtgctttc
     3661 catggagaac ccataatatc tttataatta aggaaatatt taaatgttta aataccgctt
     3721 tttatcataa tatcacttaa tgtcaaaaac agaagggaag ggacaacaac aaggaacaat
     3781 agaaataagg aaaagcatag tcattgacgc ttcacctgaa gtagtcttca agtagtcttt
     3841 gaaaactttt gactattgat aaatttatcg tatgcatttt ccatatagga gcaattacga
     3901 aacatgaata gggatgatct ttggaactct agattcttgg agctccttcc ttgctcgcaa
     3961 tttagccttg tatttcaggt tacgtggttt ggttctcaat ctatatccac agcaaggaca
     4021 ccacaaacca tcccacttta tgaaaatttc acatatttga catcgcttct gtccgctagc
     4081 gtaacgacca gagcctacgg gcttttgcgc tttatgacgg atacatatat ccttacaagt
     4141 cattctttca tttcctggta aaatatttta ccttatttca acaatgtcaa aaagactatt
     4201 taaatttata caaatatttt attaacaaaa ttgcggtaat agcaaaattt aaataattta
     4261 atgcttcaaa agctaagaaa atttcatatt taatcatata aggtccacat ataccttgca
     4321 tccttttatt ttttttactt tatatatgtg aaagacaagc ctttagaagc tgtgactcgg
     4381 agcatctcag attctagcac gactacgaaa cgactcaaaa cgtctaccat ctcgaagtgc
     4441 aattgctcta gccgacgacc tatctcttac gcctcgccta tttgcatcta tgtattttgg
     4501 taaggaagtt ccatgctcat caactatctc gacgcgaacc agatcattaa accttctttt
     4561 tatcatcaat gcaatttgct gggttatggc aatattaccg tcgcctattc taacaatctt
     4621 tctttgtgaa tcgattccta acagtaatgt cgatattaac tgaaacgcag agtgtggaga
     4681 agactcgacg aagctgtcaa tttcgtcatg cagataaatt attgatattc ctatcctatc
     4741 accggggtca acccctatcg tcaactggtc atcgtgatga gatcccataa ctttacgcaa
     4801 catttttgcc ttcagcagag caggatattt acctagttca ctatctaaga gaacatcttt
     4861 tctcttgacg atatgtgcct cgtcttctgt tgtgataata attttggcgt tagatactgc
     4921 cgcctgttcc ggagacaacg attcaaaacg cagatccatc aatttgagcg catgggttat
     4981 tttataataa ggacgaccat aaacagtggc tacagcaatt ttatattgca gcagctcctc
     5041 gctcaggggc gtttcatcaa agcttgttac acgaaataag tattaaatct tgtggaattg
     5101 cagaagccaa ttggaatatc aaaatgacgg atttctcaac ccttcttgaa cactacgacg
     5161 aatcctcgta aagatcataa tggatctgtt gcatggtggg tgggcattac tagctcgata
     5221 tttctatgtc taacacacta gttagttctc tctatttgca tcgcatctgg gacagatata
     5281 atttttctgg gaaattctgc gttcattatg gttaaacgta atacctaagc acatctacaa
     5341 tatacgaatt catccaaatt gccgcaattc taggtggtca aactcaaaat tgcctgtgcc
     5401 atgtctccat gagcatcaat caatgcttga atggcttttt ctctactgac gtttgcctgc
     5461 tggataacta ggactatatc ttcttcctta aaggtcggga catctctttc tttctcttct
     5521 atctcagaag cgataacctg aaaaatcgta ctttcttttc ctttcatctc gatcacctga
     5581 ggctttagaa gataaagttc cttagtctca gttttaataa taacttcttc aacattgcca
     5641 atgtctttca tttcaagccc catcttatct aacattcgcc tcatctcacg gttgccgccg
     5701 cgcatcatgt cgcttttata gttctcgtgg gctttttatt ccttctctga ccttcacagc
     5761 aatgccctta tgataatttt tcatatggtt acatccaatt agcgccctgc ctaccgctac
     5821 gacccgccat tcatcgacta ttgccacatc agacccgact ttgacatttg agccacacca
     5881 ttctacgtgc ttgcagaata aagatcttcc ttgtcgtaca aatggtaggg cctcctcagt
     5941 tgggattaca cagttctctc tgaatcgttc attccccaaa aactctgttg caccaaatat
     6001 agttaaggca agcccaccat cagttcgaag agtcgcgact aattgatttt tgattgagaa
     6061 atttttgatg cgcccggtcc ttctcgaata ttcaaagcta aaatcctttg tagaatgatg
     6121 tattgtgagg atgttataaa tgccagttcc gaataaggca tcgatatgat gacgtatctt
     6181 ttcttcgggg ttcaatggta atggtttcaa atgatgttaa gagagatttt atgagatcag
     6241 tacattaatt ttactcactg cgattacaac tggtatctat atagtaaaag gttacagata
     6301 tataaaaacc agagttgata ctacaacgat ggataataat gaaggcactg aattaggtaa
     6361 tcaacatgaa gaaacaagga aaatacaatt tacagggggc tcctcttata ttgtatcatt
     6421 acctaagaaa tggatccaag acctgaaatt gaaacagggc gacctcgttg taattgctcg
     6481 gcagggacat tccattttac agatagctcc aatttcaaag agaatatcta aagaacaaag
     6541 agaggcaaca atagaggttg caaaagataa tagcccatat tttcttgctc gcaagctaat
     6601 ttctctatac tttctcggtt ttaatattat caatatctcc cagaaagatg gaggaaggtt
     6661 atccagcgaa caacgcgaag ttatgaagga cgttgtaagg cgcgttctaa tgggcacgga
     6721 gatcatagct gattctgcaa cgagtatcac actgcaggtc ttgatcaatt tacttgagct
     6781 atccgtagac gcggcgttca aaagaatgct tttgattgca aaatctatgc accgtgatgc
     6841 catactgtct ttaagagaga acaatatcga gctggctaag gaaattttaa aatctgatga
     6901 cgaagtggat cgtttcagtt tctatatcgt aagacaactg aatattgcaa taaaaaatga
     6961 acatttgctc aaggaaattg ggctagacga ttctcgtaac tgcttaggat acagattaat
     7021 agcaaaatcc acggaaagaa ttgctgacca tgcggggcta atagctaaag atataatcga
     7081 aatggaaagg ccactcaata aagatatcat agaaaagatt gctgaaatga gcaattttgc
     7141 gctcgaagtg ttagatgaag catgtctatc aatattcaag cgggattatg attctgccga
     7201 tttagcgata gaaaagtctc gcgggataga cgatctagaa aaagggatta tacgcgtcgc
     7261 aagcaaactt agagatatca acgaaatata cagaatcaag attataactg aaaatattag
     7321 gagggtagcc gagtatgcca gcgatattgc agagatcgtt ttaaacatga ccgtgcagca
     7381 aaaattaaga aaaggctaaa tttgtattag tttaaaatat tctaatgtcc tcattttgcg
     7441 agctatgtgg gcgcaaagta attgatgaaa aaaagactgt attgatcgac gggatagcac
     7501 ttaatgtttg taggggatgt tcaaagcgtg gtaagccgta tacgccaccc atgtccattt
     7561 ctaaaactaa gccaaccgct tcatccaatc ctgcggctag agctactgct accagaagca
     7621 caagcactac aagaaaaatt gagatgatgg ataatactat tttgaatcct gaattttcaa
     7681 aattgatcag agaagcgcgc atgaaaaagg gattgacaca tgagcaactg ggagcgcaga
     7741 tgaatgaaaa ggcaaaccta ctcaaaaagt ttgaaacagg agcattgaaa cccgatgaat
     7801 tatttgctaa aaaattgcaa cgatttctag gaatccactt gtacgtaggc gttttagagg
     7861 attaataaat gtcaagctca cacaaagcta tcctgataac ttacccattc gaggatgcgg
     7921 caaaagaagc aatttctttg gctgacgctg caggttattc tatagagaga gtagttaatc
     7981 agcatcatct cacaagatca agatatggca ttggtatagg aaaagcacaa gaagtaaaac
     8041 aacttgtaga gcaggagaag ccagaagtaa tcatctttga tgaggtttta aaacctagtc
     8101 aacaatataa tttggcaagc atatgccaag ttgaaataat cgacagggaa tgcctaatct
     8161 taaagatttt tgaccgaaga gcaagtaccg cagaatctcg aattcagatt aagctggcac
     8221 agctccgata tgatatgatt cgagcgcgag agaaagtcag actcgcaaga gcaggggagc
     8281 aaccaggatt ttttggtatg ggtaaatacg atgccgacgt atatttcctc gatataaaaa
     8341 aacgcgcaac agttctaaag aaaaaattgc aaaaggaaga ggtaagaaga aaccttcata
     8401 ggattcagcg gtcaaaggca ggtctgccaa ccgtatcaat cgccggttat actgcggcag
     8461 gaaaaacaac gttgttcaat tctttgacag gagaatcaaa aattacagga cagggaatgt
     8521 tcactacatt atcgacatac actcgactag tacaatctaa aggaacaaag cttttgattt
     8581 cagatactgt aggctttata agtagacttc ctgcctatat ggtggacgca ttcaaatcta
     8641 ctcttcaaga gctatcatat tcagccgtag tgttgcttgt cctcgatata agcgaaccgg
     8701 cagaagaaat tgtaaaaaaa ttgaatagct gtatccgtgt aatgtatgag ctgggagtgc
     8761 caatgactaa agttgtctat atcctaaaca aggcggattt gattagtgta gaagatgtgg
     8821 aggataaatc cgttcgactc ggacttccac attcaaaaca acgcgtaata ctgatatccg
     8881 caaaaacggg gtacaagatc gatacgttaa aggacgtgat gacattgtta gttgtgggcg
     8941 atccgaagcc actaatcggc gaagtgagca gttactgaaa gtatcagttc tcagaatagg
     9001 acatagatta gtccgcgacg atcgactgac tacccatgca gcactagtct cgagagcatt
     9061 tggcagtgat aaaatttaca tgagcgaagt agacgattct ttaaaacaaa caatagagga
     9121 tgttagtagc cgatgggggg gaagatctga ttttaaggtt gaaattgttg gaggatggcg
     9181 ggaggtgata aaggattgga agaaggctgg cggaaaagtg gttcatttaa caatgtatgg
     9241 tatacacata gatgacgcca taagtaatct aaggcgagaa aatacaattc tggtagttat
     9301 cggcgcagaa aaggttccaa gggaaatgta tgatcttgcg gactataata ttgcgatagg
     9361 aaatcaacca cactctgaag tagcggcttt atcaatattt ttagacagga tgttcagagg
     9421 caaacagttt gaaaaggagt ttaaagatgc aaaactaaaa atcattccaa cagcgaaagg
     9481 caagagagtt agagaagtga gctaacatac ataataaatt ctagtcctgc tgtgcaaaag
     9541 accgctctga aacccgtcta tcgcaagggt gcgtcttggg attatgtaat gcaatagaat
     9601 gcaacgcggt ttataagagt cggcaaaaat ctgctgatcg tagggggaac cgtagttatc
     9661 ttggagatcg tgcaaagcgg ttaagaaaat gtctggacag agagttttct gattcaaaaa
     9721 attgaatttg aaagtcagtc tatttctttc tcgccaacgg gatcaccgtg caactttgga
     9781 tctggcttat actctcgata ttaattttat gtgaacttcc tcattcttag cattctttgt
     9841 taatgtaaca ttcttaccat actactacct actaaggtat gcttcaaagt catttgaagg
     9901 attgttactc gatagatctc gctgccacat ggctatgcag tctttattag gtaatggtag
     9961 cagtattttt ttgccgctga ttcaagctac taataccaat atacatcgtt tccacaaaaa
    10021 atatatggtt tgatgtggct gattacaacg tggagaccaa aggcaatact ggccctatac
    10081 aaaagggt
//
LOCUS       DAOR01000185           10454 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35351, whole genome shotgun sequence.
ACCESSION   DAOR01000185 DAOR01000000
VERSION     DAOR01000185.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35351"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    7811..7989
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3018 a         2041 c         1946 g         3270 t
ORIGIN      
        1 aatgcttaga gttcatgcag ctccagttgg ttttgtagta ggggttagtt ataacttact
       61 atatacttta tatgtaatta ctttactata ttagatatga ttatgttagc acattataac
      121 gatagtaaca agtcttcaag caatcatgca acaacagcaa tttctactcc cttagccaaa
      181 tgtcctagct gtggcaacga aaagataatc acggattctg agtctgggga gataatttgt
      241 gtcaaatgtg gtcgagtaat ttctgataaa ttgcaggaga taggaccaga gtggcgtacg
      301 ttttccacag atgagactga ggcaatgacg agaaccggaa tcccacaatc actggctcgt
      361 catgatatgg ggttatcaac aatcatcgga agaactgaca gagatgcaag cggtaacaag
      421 cttgatgcag ctatgcgtac tacgatgaac aggttagcta cctgggatat gaggtctcaa
      481 ctgcatactc ccaaagatag gggtcttaga caagctttct tccaactcga tgtattaaaa
      541 gataagctag gattgtcaga tagcatagtc gagaaaacag catacatcta caggaaagct
      601 cagtcaagga agcttactcg tggcagaact gtttctggag tcttagcagc tgctgtatat
      661 attgcatgta gagaaatggg agctcacaga acattggatg atatagcgac agcttgcaat
      721 gtaaagcgta aagaactctc aaaagatttt agagtgcttc acacaagact tgaccttaaa
      781 atcccacaac aagatcctat gaaatgtata gctaaggttg caagtaaggc taagctaagt
      841 gagaagacaa aacgacatgc agcacaaatc atgaacaaag ttacaagaga agaaatatcg
      901 gcaggaaaag atcctatggg acttgctgcg tcagtactct acttatcaag tatagaaaat
      961 ggagagacta ttactcagtc aaatattgca gaggcagcag gagttacgga agttactgtc
     1021 agaaataggg ccaaagaatt aagaaataaa cttgagttca attagtcgat cagatctgaa
     1081 taaataatca ttccaaatag tatcattgtt ggcctcataa ttgttactta ggagctagca
     1141 gtgtcaagcc aaacggctta gtaactgaat tgcaagaatc tgtaagtaac cctgtatatc
     1201 ttggtgtgtc ataattgaca gcattagtag tcttgtcatc aaagatttct gattggtggt
     1261 aaaccaaatg ctatgatgtc tcgacccata ttttctgttc ccgcagaaca gcattccgtg
     1321 tcctattgag gtcccgcgaa ctccagccgg tgaacccaca ttaaactccc aaagtctctt
     1381 acatgtgtcc ttagctagtg caaagattat cctaaatgga tttaattatt tttatacatg
     1441 tatgctaggc tcgagagcgc ctcggtaaga tctttctttt attggatagt aatttccccc
     1501 atcttgccat ttcgatagtc ttttgccgct tggagaatct cttcgttagt attcataaca
     1561 aatggaccat atctggccac tggttcgttc aatggaactc cgcctattag aagcatattg
     1621 agagcggatt tattagtaga agttgcagca acactgaatg ctactccttc tccgtcttta
     1681 tcaaagacga ccatctgtcc tgtctttgca atcttttgct tttctgcagg tccaaacaaa
     1741 ccctctccat tgataacata tgcaaaagta ttgtactcct ttggaattgg ttgtatcaat
     1801 ttagatcctg gttgaagagt aaagtgcaga taaaagatag gtgttcgagt gtctattata
     1861 gcgctagagc ctaatgcttc cccagcaata accttaactt taaccttacc atcttctgtc
     1921 tgtgctaaag gaatctttgc agaagggaca tcttgatacc ttggctttat catcttatca
     1981 tgctgtggta aattgaccca taactgaaat gcatgcaact ttccacctct acgcatgaaa
     2041 tccttctctg gcatctcaga atgaacaacg ccagcacctg ctgtcatcca ttgtacgtca
     2101 cctggtccaa gctttccaga gtttcctcta gaatctttgt gctcaaactt accttcaagc
     2161 atatatgtaa cagtctcaaa tcccctatga gggtggtctg gtgctccctt tgcttcacct
     2221 ggcttccaat ctttaggacc catttcatct aatagaagaa atggatcaaa atctaataga
     2281 tcattagttg gaaaggacct atgtacaaca aagcctccac cttctgtcgt ctctaagctg
     2341 tttataatac gagaaacagt acgagaatgg cattgttctt gtttattatt caagtgttta
     2401 tcttctattt ttgttgtcat acgtattagt atgatactaa tatactattt aactttagta
     2461 gtatgataca acttagttga taattattac caaagttggt agcgtacaga taaccatcta
     2521 taaaattaaa cggaatctta tctcatagta tattcgtgtg aatgaattta ggtagttact
     2581 attcaacgtt tttataacat gcagacaaca tctgatgatt tagtcgggaa tgtatagctg
     2641 aaaaattgct acggcgtact gattactatg gctttcaatt cggattgata actgatcttg
     2701 aagaaagacg aagtgatgcc gaattcttgt acactctgaa gttaagttat gaattaggat
     2761 tacacaggac actccctgtt ataggacaat tacaactctt caacagcagc tatcttatct
     2821 aaccattttg aaacttgagt atttggatat acttatccgg atcgtatatg tcgatgcaca
     2881 atgttcccag cgaagctttg tagcgttatg gcacaatgaa gcgacaacta cctgttgtta
     2941 caaacattat gtttaaaaga gtaagtaaaa agtgtatatc ctaatgactt taaccatcac
     3001 cggcatattg agaaaaaaac tagaaagtat tgaagagacg actactattc aagaagcagc
     3061 aaagaaaatg aaagataagg atgtgagttc attgataata gtcgatatga gcggtaaacc
     3121 ggtgggttta gttactgaac gtgatttagt cagaaaagtg tgcattaatg atgtacctac
     3181 tagcaccgtg acaagtaaag agataatgtc ttttcctctt attactatca aggctacctc
     3241 atctccatca gcagcagctg acatgatgtt aaaaaacaac gtaagacatt tacttgttgt
     3301 tgatgaaggt agtgcaaaca agcctattgg tataattact ccacttgact ttacaagata
     3361 tcaagaatat acagctgacg aggacaaaga agccattgaa aaagtattag aaaactatat
     3421 gtcatcacct gatgcagacg cctttactgg tgtgtaatcc gttgcatagc aacaacaact
     3481 ttttaacttt caactcgtcg agtttagtca cgcaacttta aattggactc aggaggatct
     3541 atccgtgaat ctttctaatg cttctttaag ctatgcatat gattttttcg atcattcctg
     3601 aatgtcatat ttctattatc tctgatttat tggatttctg tgcatctatg atagtgatct
     3661 attgtattat atagaaagtg agcattttta ctcaatactt ctaaaacatt atatgccata
     3721 tctgttaatt cagttgctga tttcatatta ataaaaacca cgaacatttg ttaaatcatt
     3781 ggtaagttaa acttacatcc atcatgatcc ggtacctgtg aggttgtctt cctgcagctt
     3841 gaccatacat gtcactagca gtattcttgg ttataacgaa aatgttagat agctgacccc
     3901 gccatatatt catacgatac atcttcccga atctcgacta tctgaacttc gcttagcctc
     3961 catcatccaa gctaagatca tactcacagc aattataagc cgagagctcc gcatcgaaaa
     4021 tcattaccat ctcatataat acccggccgt tcgtttttcg ggaggggtgt aagcaatgac
     4081 tacaaaccca tggccagcac tgccgttgac agaatggaaa gacacctatg acacgctaca
     4141 catgtggacc caaatcgtcg gcaagatccg gctcgcagtc accccgcttg cgaatcactg
     4201 gtggaatgct actctgtatg ttacgcctcg cggactcacg acatccgcca tgtcctacaa
     4261 aggtctactc tttcagataa acttcgattt catcgctcac ctgctgctca tcgaagcgaa
     4321 tgacggttcg accaagacaa tcactctccg accgcgctca gtagctgagt tttatcaaga
     4381 aacgatggcc gccttgggat cgcttgggat acctgtaacc atctggacca cgccggttga
     4441 agtgcaagat cgaaccccat ttgagaacga tcagaaacat gcggcttatg atcccgaata
     4501 cgcgcagcgg ttctggcgca ttctggcgca ggccagccgg gtgtttgcgg actttcgttg
     4561 tcggttcact ggcaaagtga gccctgtaca ttttttctgg ggagcctttg atctggccgt
     4621 cacccgcttt tccggacgtc aggcgccttc gcacctaggc gtaccaaacg tcggccggtt
     4681 tgtgatggtc gaagcctatt cgcacgaatt gagtagttgc ggtttctggc ctggtggtgg
     4741 accggttaag gaaccagttt tctatgccta cgcctatccc gagcctcagg gcttcaagga
     4801 ctactccatc cagccggcgg aggcgttcta ccatacggag atgagcgagt tcgtgctgcc
     4861 ctacgatgtg gtccgtacag cgaagtcacc tgaggatgtc ttgttgggtt tcttgcagag
     4921 tacttacgaa gcagcggcaa cctgtgctaa gtgggatcga cctgcattgg agcgtcagta
     4981 agtggtatat cggtatcaac tcgtctaccc ctctcagttt gaacgaacag acatggcatg
     5041 tagagaaaga gaaataggta ttgtttcgat tattgcaatt attctatctt catatggcct
     5101 aagtatacta catcggcgac gaaggagttt cgtgaaatag tagataaaat caatgatcta
     5161 gggattatgg cagaatatag attgatggaa gaaaagaata ttgtcaaaaa atttatgact
     5221 gaagtacatt cgactaatgt tcttgcaaat tgccaacgat tcgactcttt tgttgaatat
     5281 cttgtcggac tgtgcttgag ttaaggatta atgactatgc gtgtcggaat ctatcgcaag
     5341 ttgctaagta atcataggac tcagatttgt tcacaattta catcatcagg atgagcctag
     5401 atatcagaaa acaaaactta tgaataatca tattgtcaaa tctcatcaag gggggtaatt
     5461 ttttgagtga taagttagta gatagtataa tcctcttctc cagaacatcg ccagcagatt
     5521 tactataatg aattggtacg agtggaccga ctaccggatt agtgatagta gcataccgaa
     5581 ctcagttact tgacctggaa atgtttcgta agatccaact aacaaatatt gggtattgtc
     5641 tacatttagg catagtagcc ttcaaattac tacaattaga ggaactgcta gtcgaaatcc
     5701 ataagcatac cgaagttgat ggactgaata aaactttttt tacaggaatc tgcttgaatt
     5761 tgtttacttt atgtgaatat atctatttat cttgaaataa tcatagatag atatctattt
     5821 atagtgatat atgcatatta taagtatgcc acaattattg tttacacgaa caccactgct
     5881 agtggcacaa caatgaagaa agggtttgat tgcgagatga aatcgacttt ggagcaacca
     5941 catttcttac tatgcggttc atgttattgg tgtgcatcat atcttagctt acgcgttaca
     6001 gttgagatat gtccttcctg tttgaagggt aaagtagtat cgatgccaac aaacatttca
     6061 ttgtaatgca atacttggag ttacattaga attcagtaca aaactaaacc tacacgcaca
     6121 cagtgacatt tgaaaaacaa tcatggaaat ttaaccttaa acaatttcgt taaagctaca
     6181 atatttgtag atatgatagt gcatgttggc actcttgatt gtattaactc ccacgccttg
     6241 aaaaaatcca ttttgaagta ttgtactaat acacctagtg ctatagcgct acttctgttc
     6301 attccatgac tacaacatac taccactttc tcgcctaatt ctaaataact tataaccctc
     6361 atgatattga gtttatattc agatggtgat gatggagcat caaactcagg tagtactctt
     6421 acatctatta ttctaaagtc cgtctatgag gcctttcgca atctacataa cctggaagtt
     6481 cacttggctc tacaagacca tcagtagcga ggccttctag aattttatac atatagctat
     6541 atctattaca tttgtaatag cttgtctcta gatttgtgta attagatgag cgagctctgt
     6601 tggaagtgtc acgtgttatg tgcaacaagc acagcacctt ctgatagtcc tagaagtgtg
     6661 gtggtcaacg gactcttatt ttctaacagt attgttagcc atcgctagtt cttctgttac
     6721 tcttatgttc gctattctcc attgtagtca atgtgggtat aaatcctgtc attatccaaa
     6781 caatactttc aatcaatccc atgttatgga taccgtggga gatattagag tagttggaca
     6841 aggcattttc gatgctctca tgataaggct atgaatacat ataacaaact tgacctacag
     6901 actcgatttt aatttcgtct tccaggtgat cctactatca aagtctgttt ggtatagtgt
     6961 ttctcttacc ttcccagtta tcgtcttttt aagatataga ataactgcat gaccattatc
     7021 ggtaatttgt gctactatta ctattagcat agctcttact cgataacatt ctgtaactac
     7081 ttcatatcaa atgcaattgt tgataatcaa aaaaatctta atagcacaat aataaggagg
     7141 gcttttgtga tatcatttgt ttttcatatc atttagacat caatctttcg attgtatctt
     7201 tttattgctt cagaaatctt tatttttgca gcgtcttttc ctccccaacc ctctaccttt
     7261 acgtattttc cttcttctaa gtctttatga tgttcaataa agtgcttgag ctgcattcgt
     7321 acactctctg gaatgtcatt gatatctaga atagttgaaa aggttggatc tgtctttgtc
     7381 actggaactg caataatctt tagatcgatg ccaccttgat cctcggttga caatattcca
     7441 acagcacggc agctaatcac tgacttggcc aataaagaat agtttccaag tatgaataca
     7501 tcaattggat ctgctccacc accctcttct cttgtctgag gaataaagcc ataattacaa
     7561 ggataaaaca atgcaggata taacattctg tcgacagata tttctccact ttttggatct
     7621 atctcatatt tgatgctact cccttttgga atctctacta ctacattaat ttcgtcgggt
     7681 gggtttgttc ctggttttaa cgtatttata gcagacaaaa attctcgtta aggaaggcta
     7741 atccattgag tccgctacct ctcggaagac tttttgcgtg tgcttctctt ctttttagta
     7801 ttcttctttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnt ttttagtatt cttcttttta gtagaagcag tcttcttctt actcaataat
     8041 aatcttatct tctgactctc cttctgaact tgcgacattt gtttttgtaa ttgtttaagc
     8101 tgggaaggca tttgtttgat gaattcggtt cgtctctcca ctgattttag ctgcttttgt
     8161 agcggttgaa ccatctgtcc tattttatcc atttgagttg tttgtttctc tagtaactta
     8221 gagatggttg ctagtctaat tgcctttaga gtagtagtag aaggtgtttt tccactctct
     8281 cctttttctt gttgttgctg cagcacttct gtttcatgac cttcaactac tggtagctgt
     8341 tgctgttctt caggctggac ttctacaggt agctctttgt attcttccaa actcgttgga
     8401 atgttgcttt catctgacat aatctaacct ataatagtaa atcaaggatg ttgttcttaa
     8461 atattcagcg gaaagtatcg caacgactag tttagtgaaa acgccttacg aggtgatggt
     8521 aagaaagacc tggcgggcca tcgacgccgc tatcgaaaag aagatcgaat ttcatggctt
     8581 tgtcctctgg cgtttcgcta ggttctgaca gccaaggcgc ttcctactgt tgttttgcca
     8641 caaccaggag ggcctactac tcctgcaata tctttattgc ttgcccgcca gtttaatact
     8701 ctcagctgct cagaatcaag agaaatatcc attttgatta ccaaaaaact ctgggtagca
     8761 ttatgtctac aagtaattta tgaaatctat gcattggttc aagtactctt cgtgtaagat
     8821 tgatttcgtc tggaattatt attattgagg ttagacaatt gtttctgagc acgttcggct
     8881 cttatgttcc catctagatc tactactaat tttgtatcat ctacttccct tacaaacccc
     8941 ttttcattta cagtagctag aagcggaatg cctccttctt ctttgcttat catcttgaca
     9001 atcttacgtg gttccaatag agttagaaga ccattattgc tttttacaag agtaactgaa
     9061 ttagcattaa tcgaatgtac gtggtatcca gtatggatca ctccagtttt ctcgagttct
     9121 tgtaatgcag catcttctcc taatctatca attattgtat taatcacaaa cttgtgtttg
     9181 tgagtttcac cacgttcatc taaacaagat atcgtcttac aactagtaaa cgcctgatat
     9241 tagcataatc cacaggctaa cttccttctc gagttcgcct agaatcttcg atagaaccta
     9301 caatcagaaa aactctcgct ctctctatca catacaggac aaaatacaag tttctgggcg
     9361 tgaggtcttc ttccgcatag aatctcgtgc gcaaaccttc tatcattttt aatcttgcta
     9421 attgtgtctt cgttcattgg aaaaacgtgg gatataagct ccttcataat atactaacaa
     9481 gaatataagt atcatcatgt tactatgctt acctaaatga aatttagtga gtagcattag
     9541 ttctttgaat ccccttgaaa tgctgagttc aagttgcttc agtgggtttt gaaactaata
     9601 gttaccagct atcaaaatga tggctgatgg agtatgactt gtcacacatg ctcattaggc
     9661 cttgactagc attccacagc cacaattaaa tatcaagact atctatgaca tcattttcag
     9721 aaccgtactc attagtagaa gccttctctc ttcacatatt ccaaacgtct taaaatttct
     9781 tttaggaatt gtgattaata ccttgttagt agcaatcctt tgtgtacaat gagcccacgt
     9841 tcacatactt acaaatacat taggtaaaag gattgggaac tataatgttg atatgaaaac
     9901 ggagcaagaa aagacgacta tcattatttt tgcaatacat tcgtcgaatg tatcgcatag
     9961 tttttataat aatattgtaa catgcaaatt aactcatgaa ctacgtaata tctggaaaca
    10021 tcaaatttaa attgccttac aattaactct agattgaaag attagataag ttaatgagaa
    10081 atcctttcaa aagatctggc acaccctatg ataagggaat ccctgctagg gaaccatatg
    10141 gatatacagg atcaagatca tcagggcctg tcttgtcctt taacaaatta aaaattgtag
    10201 caggaataat cacatttgtt gtcattatcg ttttcatgtt tgaatcagta gttgttgtag
    10261 aagcaggaca tagaggagtt gttctgtatg tgggggcagt tgaaaaccgt gtattaggag
    10321 aaggaataca ctttatcatt ccttttgcgg agcaggtaat tcagatggaa gtacgtactc
    10381 tcaaatttga agctaatgca tctgccgctt ctaatgactt acaagaggta cagactacaa
    10441 tagcattgaa ctat
//
LOCUS       DAOR01000186            8609 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20459, whole genome shotgun sequence.
ACCESSION   DAOR01000186 DAOR01000000
VERSION     DAOR01000186.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20459"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2611 a         1594 c         1522 g         2882 t
ORIGIN      
        1 ttcggtaata tacgatataa tagagccgga aaatgaggtt taaagtaaat gccagaaaaa
       61 cttactacta caattaacaa aatttcttct gtcccaaatc ctaccaattc tactataata
      121 taggaattct ccgaatatat gaaagggaat ggatcttcgg aacatcatca aaacaacaac
      181 ttaagcggtc atagcatttg cctgtaactt ggtagcgaaa atatgaccct acaatatgcg
      241 tttccctgac gaactaagga aaaaattatg aatcaatgcc aaatattcat gatcaaatac
      301 cacttgttcc ggaatatcaa tatattgttc tatatgagca ggcaaaaaga gccgattagc
      361 acggattact aacgcattga gaactttcct atcaatttaa aatgcccatc atatgtagga
      421 tagctgcatt aaactcctcg atgcatatag aagatatgtt tctgaaccag aaaattatgt
      481 gcccaaatag tcagtactag gtttttgacg tgtcaggctg aacaagtaac atacctacta
      541 tcaccttcca ccttcgtacg cagcattggc atctcatgta gaatcaaagg tagtagcgtt
      601 ccaaaagttt ctaacagaac aaaataatct actagctata caagtagttg caaaccaaac
      661 agttgttcaa gctcaaccgt ttatacagac aataaaatat agaaatttgg taatagattt
      721 aggtaatgga gtaaagacaa atgctcaatt gactcttcct gctcttggtg acggtccatg
      781 tccaggtgtg ctgcttattc ctggttctgg agcagaagac atgaacgaaa ctgttgccaa
      841 gaatgctaaa ccgttttggc aaatatctca atatctgata gtatgagcgt tgactaaact
      901 aacttattac acctattatg gagtaatgag actagtctag tatatctaac ttacttccta
      961 tgtcctattt agcataacca taaggttcgg acataatcta tcatgcgtga tttgatttca
     1021 ttattttttt agcatcattt agatatatag taagtttaca cattgtcgga gacatataaa
     1081 gaattgtcca aaactctagt tgctatggac ggctcagaat tatcaatgag agcggcagat
     1141 tatgctatcg caatcgctaa gagaggagga gacaacactc aaataattgc attacatgta
     1201 atacattcac aaatcaaata cctctattca gcgttgatag cacctactgt aagagagaca
     1261 gtcattgagg ctgcaaaaca agaagctcaa aaatggctat ctaaaattag acaaaaagct
     1321 gatgagaata gtgtgaaatt aaaaacagaa tttattgttg atccaacatc aatagtagga
     1381 gcagtagtag attatgctga acatgagggt gttgatttaa tagtaatagg ctcaagagga
     1441 ttatcaggct ttaagaaatt attgctggga agtaccgcct cgggcgtagt aacttatgcc
     1501 cattgtccgg tattagttgt aaaatgaaca gaaatatacc agctaactta atttgtcatt
     1561 atcgtataat tatttggaat gtagtgatcg gatatgtcag caacagagat aaacataaag
     1621 aatatcctat taccaatagg tggcgctgtt ggtaccaatt ttgtctcgaa gaagattggt
     1681 caatttgcaa ttctttatta ttagttatct gccttacata cacatttccc atgtctaaat
     1741 tctatgtcgt tttcgggatc aaagcgtttg ggaggcacta tttctatagt ctcagcttta
     1801 ccgaaatttt acatttaagc cgaacctgag tcatttctta gtcctcaaca ataaaatggt
     1861 tctttatcat ctcataaggt aaatacattt taccatgatg tttgtctata atatgttgtg
     1921 ataatttgat tatctgatcc cctgaagtaa attccatatt acaccgtctg cagtaaacct
     1981 ggtcattgca ttctatcctg actccatatt tctgattatc gaagttcatt gatcccaaga
     2041 ctgtcataac gtgaaattca ttaaactttg ttacacctaa tttctttttt ctttctgtat
     2101 ttatcctttc caggataatt gatactagct catcttttgg atcttgagga accaatctgt
     2161 ctgtacaggc tttagtgagt acagatgcat tcattcgttc gatatagtaa tccgcttgtc
     2221 tcacagattc ttcgacttca tcggccatag aaatcaaaga ttgaattctt tcaaccattt
     2281 ttcgctgttc aagacgtggc gggagggggc caggcataag ggatagcttg gtaccattta
     2341 cattcgcttg gcctacttgt tgggttgcaa cagatgaaat atatattcga ccataatgtg
     2401 aatcaataca cccctacagc tgcatgaatt gcctctgaaa gaattgcgct tatgatgcat
     2461 catctgtctt ttccttaaga agcttagtct gcaagcccct tcagatattt ctattgcctg
     2521 agtcacaggt gacaagtctc actagatggt cgtgtcgacg tttcttttta ctgaggatct
     2581 tttcatcctt cgcacttcaa agtcagatgc agttactgtg ttaatgacgt ttgttgtcaa
     2641 ctgacaagac ctgtgataag cttaagttac caagctgaac taatcagtag ttgttgttgt
     2701 tttattacct ttcttactat caacattctt ctttgatact ccttcttctt cgaggtcatc
     2761 agcccctttt tcagttatgg taattctagt atcagaatat ggatgatatg gtgaggctaa
     2821 tcaaggcttg ttttctattg ttgttgtaac ttattttctc cttgcaaatc tattagtata
     2881 tcgtctgtcg tctcttctat ctttactaga tatgaggtaa gctctgtagc ccaaacagtt
     2941 gcatctgaaa ccgttgcatt tcggatatca aaaacagcat aatgccaagg gcatgtaaga
     3001 ttgtatcctt caagtgtacc ttcttccaaa ggaccacctt catgtgagca tatcgaatcc
     3061 atcgcatata cattaccatt taccatagcc aagacaattg atttaccatt aggttcaatc
     3121 ttgagaagac caccttcctt caaatctttt ttgtttgcta ccttctgata ttgttgctct
     3181 ttagccatat aatcaatggc agacctttct atttacatgt ttatctaagc agttggtctg
     3241 catgtcgatt gcaagtccat tcctttgatt gattttatag gttatgcttt atattatttg
     3301 gacgattaaa tttgaataag attagttgaa aatagtaatc tcgattgtaa ttctgatggt
     3361 tgctgtatca ttcctatcac taaacgtaca gactacactg ttaggagctg caataaaagt
     3421 acagcatcca tcaacgatag ctgtttttca tcaggcttct cattctaata ggagtagtgt
     3481 taacatgagt ggcactaata gcagtggtac caaactagca attataaatt ttgatgacgg
     3541 atacatgagt caattcacaa acgcaaaacc cattctagat aagtacgcct ttaaggcaag
     3601 tttctttata gtctgcaatt tcgtgggaaa gacagcaaag gaaatgaatt caagctcggt
     3661 aatcaatttc gctggaaaag gtgttgaaca aatgacttgg aatgatataa tcgcattata
     3721 caagcaggga caccaggtag gagctcatac tatgaatcat ctacaaaata tgacaaatat
     3781 gcctaaaagt gaattggact atgaaatagg tcattcaaag caatgtcttg ctggtcatgg
     3841 aatatacaca acaacctttg catacccgtt tgagaacggt aaagacaatg caacaatagt
     3901 caaaaaggtt gctcaatatt atagttatgc tcggtcagga aactaccctc ttgtgtttct
     3961 tcattgcgat cactttataa aatatagcca tcaggctgat tgcagaactt acttaccaaa
     4021 tggtaagatt tcctacgcca acagatattc tattgtaggt tggagtcatg attatgatag
     4081 aatagcttat ttttacaacg atcaacaaat gcttcatcga tttattcaag ttgtcaacgg
     4141 ccaaaacaaa tacaacaagc caggacaacc agtagatgcc attccaataa tagtatatca
     4201 ccgaattgat aacagtgggg ccagatacag tactactgtg tctctgtttg ctgcagaaat
     4261 gcaatatcta catgataatg attttgatgt tataagtatg actgatattt tatatgataa
     4321 tgtgacaaat tcattttatt tgaataaccg atagctttgg ttggttgtat gcgggatagg
     4381 tttggcaccc tacgatacaa cacctttgat gttacaccct tatgaaatgt ccttatattt
     4441 gtataggcaa ctcacaataa tatcacaaga atacacgagt agtccatgcg aataaacata
     4501 ttcagcagca acttgtactt cttataataa gagtagctca gaatttagtt ccggagggaa
     4561 ttttaatgta ttttaatgta aacgaccata ataaactatt ggtacatgca ttaagttggt
     4621 caatctgttg atcaactttt atgctgtttg ttaatatgtt gatatttttg ttgttatgat
     4681 gtcctcctgc taaagccttg atgtggtatt gcaaacgcaa ctcaggtggt tgtttgtttt
     4741 catgggctca atttagttga agtttatcct ttagatcctt gaacccgttt cttaaggtca
     4801 cgtctgttat cccgtctgct tgtgcaatat cttgagttct aatattatgt gtccttttta
     4861 tatagagcaa tattgggaag aactagattt tcctccatga attatgcctg actacattgt
     4921 tttcgaatag atttgtgaag tttgcagccg ttcttgataa ccacggaaaa ctaattgtcg
     4981 gtgaatatag gaaaggtatt caaacctact ggcgagccga tttcatattt taacaataat
     5041 tatcatcatg acccaagtta tcttttccat ttggattacg tggttcctgc tataaagaaa
     5101 cgaagagttt gttggccaga ctctacagga ctgacgttat ggtcgatgcg gctattgtat
     5161 gtagtaggtg gcgtaggacc aggaagttgc aagtatcact tcaaatgatg tcgtcgcaca
     5221 cttctccacg atgtcgtcga ataacaatat tattgtcttc tctttttgct atagtcccat
     5281 gtagaatagt aactgggggt aatttctaag ataaccgaat ccccatcttt aacactaacc
     5341 ataagagatg tcgccatagg atgtgcgagg cttccaaggt atctgaccat tatcttttca
     5401 gctatcggta tattatgatt gatgtcttca tggattttca ctttcccctt gcctctaact
     5461 cccttatacg gtggattcgg atcatcaatg taaaacttat tgacatcaaa gtaataccat
     5521 gttggatgaa tgtttggatc tcctttattg tcagtcgtgc caatatggat attgagttta
     5581 ctattgccca aaaagttctt tatttcttcc acagtcaatt gtgggccaaa acctggactg
     5641 gcattcagaa ttctcatatt attagatgta atacggaaaa cattcgtata tttgtgatca
     5701 taactggacg aagctgctca aatttcccat ttctccatgt cacgactata ttctatacat
     5761 gcattatttt ttttatcaat tgcatcaaaa gcttctgcct agcgccgccg ctccgtgagt
     5821 tcatggccag ttgaatcgtt gtgcttttta gccatttgta tatcaatgaa ctccttgttg
     5881 catgggaggc aatgatatgt tactctcatg tcagaaatga tgcagcaaac taacaaaaag
     5941 tttatgtgtc catacttgat agcattttaa gaaaaggatc gcgaatattc aaggaaacta
     6001 cagatgttat tactttgtat cttgcaggaa gaaactaggt tttttagttt atatagtaag
     6061 gattaatcct taaccaatct gtgtcaagaa gaaataatag agtaggattc ctctttattc
     6121 acacactctg tgatcttgat aatttgataa ttagttgatt gatcattttc tgttgctgga
     6181 gtattagggc cataaatagt gattcagtcg agtagttggc acctttggca atagctgcat
     6241 caccagattt cctacactca ttcaacattt cgtgaaacag aattctattt tcttcacgta
     6301 atgcatattc aaaacctttc catgattcaa tctctttggt aagaaaatct tccttattct
     6361 tctccgtgac catgtgctga cgttatttgt ttctcttcga tttaagaatt gaaacactaa
     6421 tgagggagag gggagctgtg cctgttttaa cctcacaact ctttcttgtc tatttaatcg
     6481 tgtttttaac acaaaaaact attgattatt tgaaaggatc tgttaaccgt tagacttagt
     6541 ctttcaaaac tgcgaaggga tagtggattt aagaggctct atgtttaata gtatggtacc
     6601 gtatattctg aactaacacg tacgatactg agtagttgga tgatgggaca agaaggtgat
     6661 agtcagtcga aatgacaaat actgttatta taaacaatct ggatgaagct gtacaatact
     6721 ttatccaaaa gaagacaaaa gaaaatgtag agttgtgtaa gatagagcct cattatcaca
     6781 accacggcct aaccggtgag ccaatatatt acattttaga aagtatagtc gttcgttgct
     6841 aagaaaagaa aaagaagttt aaggtccgct tcactttgtc gtcgaagttg tcgttggtgg
     6901 tgtaactgga tctagaggat aagccacaca ctcttttcca tcagatattc caaaattccc
     6961 tttcgcccca tccaaatggt gcatctcaac atatttcatc aaatttttgc aatatgaaat
     7021 attagctttt gtaatttcag taataaatct caatcttgta gctctagtag cttttgattc
     7081 caacattgct ttctttattg cttcaaatgc aaaaggtacg actgataatg atctaacgtc
     7141 tgcaaaataa tcacatcttc tatttgcatt ttgaagaaga tgcagatatg tgttaattat
     7201 aacttcagtg ttatttaata actcgatcct cccggaatta ttatacatat ttgcatccga
     7261 aattgttaat tgtcttcaca aaacaacaat aacgatattc atcatttaaa gatcctttga
     7321 tggatttgac aggttttttt agaaatattg cagtcatgat gccaattctt tctttatttt
     7381 ttttactaaa tcatctaaag ctaggggctt tgaaatgaag tatttagcat gacgcaacat
     7441 ctcaggaggt ggagtcctta ggccctcatc atatacttca gatgctgtca gaaaacatac
     7501 ttttaccttg ttatctaact tccttatttc tttatacaat tcgtacccat tcattttagg
     7561 catattaata tcaagtatag acaaagagta gaaattaggc ttaaaatttg agagtgctaa
     7621 tacaggatca ttaaacgcat caactttgaa tccttcatct tccagaccca tcttgaaaac
     7681 ggaggtatta tcaggctcgt tatctacaag caatatttta ctgttattca tgatgattac
     7741 cgttatgtga taatacagtg attattaata cggttattct tgttctcaag aggctatctc
     7801 ttcctttact ttgtttacaa aatcatctat ttcaatgggt tttgaaataa agcattttac
     7861 attctctttt ccatatgctg acggcattga gaagaaggac tgaaacctct ttttcagctc
     7921 ttcatagtaa atctcatatg ctgtgataaa gcatactttt actttatcat ctatctttat
     7981 tatttctgta tataattcaa agccattcat atttggcatt ttgtagtcaa ggaggatcag
     8041 atcataggaa cctttcttga actctgacaa tgctaaaaga ggatcgttga agacatcaac
     8101 tttgaagcca ttatcttcta acgccatgct gaagaccatc gtaagatcag gttcatcatc
     8161 tacgagtaat attttcttag tattcacgat tgttgtcgtc actcccttca aggcttggtg
     8221 gcggctcctt ctgcagttcc tgctgttgtc tctccttccg taggtctaag taatggcagg
     8281 ctaaatgtaa aggtagctcc tctaccattt gcatcagaat ggttatttcc agcccaaatc
     8341 ttacccccgt gagcttccac aatgctcttt gaaatgaata atcctagtcc ggtacctgat
     8401 tcagattttg cgacaaactt tgaaaataac ctagggatta tttcaggatc tatgccttga
     8461 cctgtgtcct ttatagtaac taaagcctta ctatccttta tttctccttt aactgttatg
     8521 gtaccttcct tggtgaattt aatggcatta cttaagaggt tagaaatgac ctgacttaaa
     8581 cggttcttat ccgcttctac atagataat
//
LOCUS       DAOR01000187            8847 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_32016, whole genome shotgun sequence.
ACCESSION   DAOR01000187 DAOR01000000
VERSION     DAOR01000187.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_32016"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    191..493
                     /estimated_length=303
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2745..2927
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2540 a         1510 c         1674 g         2637 t
ORIGIN      
        1 tatattcaaa ctctgataaa ttcattagaa agattatagt tagaatgtcg cttcatatat
       61 taccgagaaa aatacttcta ccaactcaga tatcttttat ccaatatgaa taacgcaaag
      121 agttagacat ttgcagaata ataatttggt gtaggtttgg cttgaagagg agaggattag
      181 ggatagtttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnntgtggtt cataattctc ctatccccaa tccctttcaa attataaaaa
      541 attgacggac attggttctg gagtttattc aataggggtt gttaaagaaa atccgatgtc
      601 taccacagta ttatcactct tcagaaatca cccccaggtt ttgaattgtg tccaaattta
      661 ctattctgtc acacccagaa attggtgcaa gactgccaag ttggcttttg actatgtcag
      721 acgatatatg atccagattt gctccaaggg tcataactgc cctttttgta atacatccta
      781 cagggcaatt ctatttcccg cttgtgtcag tgccaataga gtatacaagg ccactgcatg
      841 attggagcag gtatccttgt ccctgatata catacagctg accaaggaac gaagactcct
      901 agttcgttat gccatacgcc actctgtttg tgaatatgaa tattccttaa attgcatcat
      961 agtcaaatgc aaattcctta aattggctac taactcacac ggcagcatta caaccgcccg
     1021 gaaaagctga agaatttttc ccagcattgt gaccgttcga atgagccaga gtagtagtgt
     1081 tttgtgttat agagatccta gcagcttgca tttgtctaga atcaaatccc aaacaaaacc
     1141 tgtttgctcg tgttgtatta cataacgaca tttcaggaca atcggcatct aggccagcgc
     1201 gtacgccact ttcatgaccg tctattagac tggttggtgc gcaagtctga atgggggttt
     1261 gttcatgagc taatggaatg gcgactaaaa ctactacagg taaaagaaaa atatagagta
     1321 gaggagaatc cgatcggaat cgcttttact attccacgac cttcatgatt attgtgtacg
     1381 taaccgatgt tggtaacaat cgtgttgaaa ggttcaccag taatggctta tttgtgtctc
     1441 aatggggatc tataggttcc gctcctggtc aattcaataa tcctgggcgt gttgctgtag
     1501 atccatcagg taatgtctat gttgctgatt ttggcaatga ccgtattgaa aagtttgatg
     1561 gtaatggcaa actcattact acctgggggt cactgcggca gaataacgga cagttctata
     1621 atccaacagg tatgactgtt caatttcctc aaggcaatgt gtatgttgtt gatacaggta
     1681 accaccgcat acaagagttt acaactgacg gtaaattcat caataaatgg gatctaccag
     1741 ctgggacatc tgtaattagc cccattacag gaatcgatat tgatgtcaat tcagcaggga
     1801 ctatattctt aaccgattcg aatgcaggta ccataagtgt aatatctgct acacctgtga
     1861 cgcagaagtg aataagtggc gggtttcacg aggttaagat ttttgtttcc atatgccaag
     1921 tccatgtctt tgaatcgcgc taccattact acttagaact cgaaatataa aatgcgattt
     1981 atataataat aataataata acgactttac aattgcaatc attccagccg tagcgctcac
     2041 aacgaatcta caaaatgcaa cttggctcag aaagcagcca atttgaatag cgtgcttctg
     2101 atacaatatt attagttgtc gttatcattc acttgacaaa aaatgacaag tgttgctccg
     2161 actcgtagtc agcctgtatc ggaggcattg tatgatccgg tattctgggt ccatcatatc
     2221 gtgacgcatt gatggcgcct cttccaagca ctgtatgaag atgcttttct agtcttacta
     2281 tgataagaat atctgctccg cgatcattgt acctctcgca gccaagctat gtgtatctct
     2341 tttatggggg agaaatgggc ataatcaggt agcgtgcata atctgctaga aatcgctctt
     2401 ttaggtaaac tgtttacaaa tgtctctcaa agctatccgt ttatagctag attcccattc
     2461 taggatatga acaaaatcaa ttttctattt ggagtaacta ttggcataat gacaatgcta
     2521 ctagtatcaa ccatgccaac atcacaccta gtgaaagcca gttcatgtag cagctcggct
     2581 agtgctggag gagtatcgtc ggctcaacga caagcctttg ccaggcacgc tgctttacga
     2641 cagcacactg gggaccaagg ttgctcatca gggagtgtag ctgtcggtgg tactaatggt
     2701 ggtacgaggt gctttgcagc agataatggt caagctcagc ttgcnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct gttctacgtc
     2941 aaaccaaaac caaaaccaat ttctagctaa ataaataagg cattggtaag caagcagtaa
     3001 catgaataac agaggaaagt accaaaaagg tggatgactg cgaccttctg ctaggagtgg
     3061 ggtcgcagct attctcagcc aaattggcgc caccatattt ttctatgttt tgactctacc
     3121 ataaggattg tgaaagcttc gtgtcactaa attgacctaa acgaaggtta caacatcacc
     3181 ctttagaact ttgtcgaaag tgagatatct ttgagttgtc gattatcaat tagctcttct
     3241 attatcttat tacgttgttc ttttggcatc ttactatcat ttgctactcc aagagaatcg
     3301 attgctttaa gtttccaata gttcttgaga gcagttccaa gcaatctgta agaatcaaag
     3361 atcaccctgc caaatgctgt aacaaaatac ctcccttttt gtcttttcac caaaccagct
     3421 tttacgaggc gagatattct tgaatagtat tgctttttgg taagtttcaa tcgagttctg
     3481 agattctcgc tactgtctcc actttcaaga aagatagtgt taaaaataag ccgggttttg
     3541 tcatcagata ttagttttat aacccctgct atgaactttg aattctggtt ctttgctgta
     3601 tcgacatcgc catcacgatt tcgttttgca gtatttactt tgttgccaga aatcacattc
     3661 aagcttcctt ctagttcgga caatgttatt aattcatatc tggagaatta tgagcagata
     3721 taccgcgata agagtcggag taatgttttt ttcctatgta gctgatgttt ttctttttac
     3781 catttccata tgttgttact agatatttcc gtatttacga ttagcaggac gttttttgtt
     3841 tgttagatat agcataatta accctaatga aattacagaa ggcaagtttt aggaagagga
     3901 tgcgttgaga aatgaagtat tcttagcaag atgccgttgc gggcacaaca taaaatatta
     3961 aaagcataaa atattcacga tttacaatca aatcgaaatt ggcgctcctg cggtatatct
     4021 tggccggtct ttctcaagtc acaactagag aagcattatt acaaagccat tatcggatta
     4081 aaggaaattt ggtaccgtta ccaatgtacg ctccaaatcc cgagttttct cttttactgc
     4141 gtctgtagat aacaaatcct tgattagaac gtctttaatt tcttggttgt cgattaacat
     4201 agaaataacc ttgtcgtttt cttcccttga aggcatctcc aatgaatcga ttgccttcag
     4261 tttccagtaa ttgttgagtg caatttctat ttttgcttca agactcgtct gggcgctata
     4321 aattacctta ccaaatgcag taagaagata tttgcctctt tgtcttttga ctaaaccagc
     4381 ttttatgagg ccggacatcc ttgaatagta ctgctttctc gtaagtttca attcagttat
     4441 gagaatatcg ctattcgagt ctgctgaagc gatactttta agaatattcg atgctctatt
     4501 atcggaaata gcctctaaga tagctagctt ggatgttaac atgcttgatc tctcaggaaa
     4561 tgagaggata gattataaag gcgtattggg taaaatccaa gaaatgtagc cattagttgt
     4621 aatttttcta tcaactccct gaatattgta agcatgatgc taaacaggaa atttgcagca
     4681 cctgggttct tgcagatatc tacccattga tttttcaatg cacaaaatgt aaccagcaaa
     4741 accatttcga aaccaccaca tcgcatcctc gcatgcaatc ctagttgggc tattagtatt
     4801 attttgtcgt gacttacccg atgaattcaa tttggctatc caatctgcta ctctccttgc
     4861 cacgacttca taatctcgag gctcctgtaa gagctcttca tatagcataa tattgttact
     4921 gcaactaaaa taatcgttag ctggtttttt aagtgaaaaa aggcctcttg aagcgttgcc
     4981 atttatcatc gtatcaaaaa atgcagctaa cattttatct tagaattatc atttgtgtag
     5041 agaaaggcga tatgactatg tgagggaatt ttatgaacaa attcaactgc gtcaacaact
     5101 tgaatacgtt ttagaatatc accgactttt agtcgtggat tacgaatctc gccgatagta
     5161 atattcggat ctatcattac aacctgtatc agcaattctc ttttaagact acaaagaatt
     5221 acttctgctc cctttccgag tactttgtat aatgactttt caaataagtc ataattcgta
     5281 agtaattctg cttctgagaa tccactgata gaacaaatat gatctagcaa atctttggaa
     5341 gccttttcac caagcaacgc aatggaatta ttggctgcgt tatgaagaat ctgctcagcc
     5401 ttacaagaaa tctgaaaata tcctagctca tctaaaatgc tcggatattg ccgaaaatgc
     5461 acgtcttcat ctgagcgatt cgacaagaca aggtatatta tgacaacatt tacataataa
     5521 tgatcgtatt acactaatca agtatcttct accattcgtt atttcattac atatcttctt
     5581 ttctgtttgt gaggccctga agaaatgggc gatatacggt gtattgggtg aaaaaggagt
     5641 tcagttatta gcgggtcagc cacaggatgg ggagaaatag atatgacaga caatcatgaa
     5701 ggatgttcaa agttgaattg ttgtaaacgt tcgttgcttg ccgcaaacat accggcactt
     5761 catgggctat tatttaccac taatcagtcg aagtgtattt actccgatga aggagtagga
     5821 gtaagtcatg ttaacgtagg tcctctcatc ggcgactgtt tacaggtttc tattgaaaag
     5881 gtaagtgaaa cggtactttg attgttggaa tgcgaatgtg cgggatcgga attatcgtcg
     5941 atggagaatt ctgatgatta ttgttactat tattagcatt aggagagagt aggtggttaa
     6001 gattattgtt actggtggaa gtagttgtaa cagggattat tagaaacgtt tttgatgctg
     6061 tgttattctt gtatccatgt gcagctactt ctattattgt tttgtactta ccagttgtgt
     6121 cgccttgact aactgtccag gaatatgagg acttgccagt attatctgtt gttcccgcta
     6181 actttttgaa tgatccccct gatgggtctg taacccttcc taatacagaa gctcctacta
     6241 ctggagaagt cgaatttgcg tcagtaactt tgattactac actctgttta tcgcctgggt
     6301 gaacagactt gttggctaga tgaatggaga ctgacatcgt gctattgcta ttagttttat
     6361 tattagtgtt agcaacgttc gatggatggg aggaggaaga agtaattgaa gaaattgcat
     6421 taccggtaga agtagtgttt acatttggcg tcgttgaatt cttgcctgca ttttgttgtc
     6481 cttgatggtt tgcgatggga gtaagaacag tacctacgac gcctgttaca ttaacactac
     6541 tatgtgcaat caaagttgga tcgttggtgc atgagaattt agcagttatt ttattttgtc
     6601 cttgtttaac aatagtatat gcaggtgtga gggtaaagtt ccactttgaa tagtctttgt
     6661 gtcctgcatc aacgttggca aaagcgagat ggtatggttt aacaccattc acaataactg
     6721 aaacattaca gtcagaagtt gtgttatcga cggatgttcc agaaactacg aggttcttgc
     6781 cagcggctac ttgttgcgct tttgcaggtg aggtgatttt tacgacatgt atcttctgac
     6841 ttggttggtg ttgttgttgt tgttgtacta ttgaattgtt agggacagca gaatgttgag
     6901 tattcggaaa aatttgggcg tataccccgt ttatcacaag cgtcgaggta agtagagtta
     6961 gaattactgc cccaacagaa aagcacaata attttggtaa acgcgaattt aagtttagta
     7021 gtagaattat cattcttata ttataagaga atctcaggca ataataaaag taaagtgaat
     7081 ccttgacata gaagaaatac actacattat gtatcgtatt cgtgtgtaga cagaaatgaa
     7141 atcacagagg gcactagttc gatgtcacgg aacctgcgca tggctataat ccgccaatac
     7201 gtctttccaa cctgaaacat tagttcgcct atcgagcttt tttagaatat cagacatgat
     7261 atgagatgta ataacatatt ttagtaaatg tatgcttatg ggaactaaac ccttggctga
     7321 ggaacgacag cgattatgtg aaaaatgtta caacaagact tttccttacc aaaagtattg
     7381 ccaaaaatgt gcgagttata acatccacca taatgacagt tattgtaatg tcgataataa
     7441 atcaatgata aaaagatcag aacctacaag gtatgcagtt cagttagcca ggacacttta
     7501 ctcgctgggc atagaaatta cattagaacc tgaaatctgg tatacaagct gtaattttta
     7561 tactcctgat cttctagtta acaatagagt catcattgaa gttgatggtc cagttcatga
     7621 agatctgaaa attaaaaaaa atgatagaat aaggcagcgt gcattagaaa atactgggta
     7681 tgtagtatat agattcaaaa atcaagaaat tgtggagtca ttgggagatg taataataaa
     7741 aataaaatat attttatctg agtcgaaagg cgttaatccc aagctattcg aaatagaagt
     7801 tcctgaaggc aacagaatgt ctaatatatc agaaaatttt gtgaaggcat acgcaagcgc
     7861 actgaattct actctaataa caattgatag atggaatgca atctatttca aagggtttct
     7921 ttcgcactac gaccctagtc ccataggcaa tcggtgtgcc atggagaagt taatctttac
     7981 tcttcttggc ctgagtttgc gttcgaagga ctaccgagac gaggcagtta tagactttga
     8041 acactattcc atcctttttg acaaaagcat agctattatg aatgagctgt ttggaaatgc
     8101 ttcagagata gaacttaaga atgcattcaa cataactgca acaaatttta taaaaaatct
     8161 gatattttac ggcaaaccac gtgtggcagt acgtcgtcta gtttggatca aggattataa
     8221 gagtatagtt tttcatatca atgaatttaa tgaatatttc ctcaagtttg gaataggtgt
     8281 tgaagagtcc gaagtaaaac ttgagtgtct tggtgaatta gataagattg aaggcggcct
     8341 tcaagaaaga gcaagattta atcggcttat taaaagcgat gagatttcta aatcagaaat
     8401 aaaccgattt tcatggcttg aaatatggtt agaagaggca aagtctctta attggttaag
     8461 tgagtggctc gaatacaaga aatcactaac caacaggctt tagcgtacag acaccagaac
     8521 cacaataata acagtaatag ttctgtagaa agtgaatttt ttgattgcct atcacttgat
     8581 ctattatagt gctaccttct ttgattgata tcatcgtttg cttttaagca aatagtgacg
     8641 ttaaaatcat gaaaattaaa attatgctac tgagaatcta atatgatatt atgaaaattc
     8701 tgattgcaga agacgattac aatattgcta tgcaatatag gattgcattg gaaaacagaa
     8761 atcatgaagt tttgcttact tatgatggcg aggactgtct aaagagttat catgaggcat
     8821 ttgacaaatt agtatctgaa caacaac
//
LOCUS       DAOR01000188            3375 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_81107, whole genome shotgun sequence.
ACCESSION   DAOR01000188 DAOR01000000
VERSION     DAOR01000188.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_81107"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          929 a          749 c          609 g         1088 t
ORIGIN      
        1 ccccatactt ttattccttg gaaactgcca actacctgcc tctttgattt gaattcagga
       61 tcgataggac cttcagcttt agaataatta gttccagtgc cactaagtga tatagtatca
      121 gctcccgctg ctgtagccgg cgctttcggt gctaccgtta tcgttggctg cactagtttg
      181 ggtgaactag ctgggcctgc aggcggaggg atagcggctg cgcttctcac tttaggattc
      241 tgtgccaaag gagtcaattc ctccaatctc tttatagcac gctcgagaga aagcttttcg
      301 gctttctgat aatgggcaac taatttatct ccgatttttg tatttcttat aatagtgtca
      361 ataaccatct tggcattctg atctgctttt agcctgtagt ttttaaccca gtaacaatcg
      421 ccaaaaaact ctaacggttt gatctgataa attaaatcaa ctacttcacc ggtcactgcc
      481 tctactgtga catttagatc agccgataat gtttcccctg tcaaaggatc tagacctttc
      541 tccgtccatt tataagttgc atatatccta tctgcatagg catccatttt gaaaccagaa
      601 ctttttaaat cattgctaat actggaaata tcggaccagt ctgtaagatc gaccggcatc
      661 ctaaagacac ccaatctgga agcgtgtaaa ggatagacgc ctctttttgc aatttcggat
      721 aacattatcc atactcgatc ctttagcaga gcagacatca aaataactct gaagctaaaa
      781 gggctaaaag gctttctatg aattatcgtc gtcagggcaa gtcatttctt gtatcgcttt
      841 atacctagcg tccatctcct ctgctatttt tatgacgtct tctacttgat attttgactt
      901 cggaaaatac catctaattc cgaccttatg gccaatacca tccatatcgt gaatcattcc
      961 tttgtccgca tctacattgt acttatcatc attatttcgt tcccgtactt ccagacctct
     1021 tttcgtttta tcttctaata tgagattcag gttatcatcg tagataaaat aaaatttgcc
     1081 ctttattaga ggagatctat ccgcttgcat agatttgtct tcgtctatct cacttatttt
     1141 tctattgggt tccctatcat atttccccat tcagtccatg atccatcata gtttcgtact
     1201 gaaggatatc caagaaggta ttttaggacg aaccaagtat gtgatgacct ttcgcctatc
     1261 ctgcaatatg cgatgacctc cttgtctgga gttatccctt ttccatcata aagagatctg
     1321 agatcttgaa cgcttttgaa agttccatct gcctctttaa ttgcttgggc ccacggaaca
     1381 tttgctgctc ccggaatatg accgcctctt tgcgcatgtt ccatagggta ttctggggga
     1441 gccgtaattt cccctgtaaa ctcttttggt gagcgaacat ctaccaagac ggtttcagcc
     1501 ttttccattg ctcgcttcac atccggcaga tacgcacgga ccccttcatc aggcggttgg
     1561 gcaatgtact ttgaggaagt gtttccttca gcagctggcg tgggatcttg ttttgtatat
     1621 tctcttttct ccatttccca ctttttcctg cctccgttca ttattttgat ttttttgtgt
     1681 ccgtaatacc gaaaaaccca gaatgcaaac gctgcaaacc agttattgaa atctccgtat
     1741 aatataagct cggtatcagg agtagctccc actttcgaca taagagcctc aaactgctgc
     1801 ttattgatta tatcccggcg atctgcatcg ttgatatctt ttctccacca aatcaaatat
     1861 gcgtcggcta gatgaccctg ccgatatgca ttttcaggat cgtaatctac ttcaaggatt
     1921 cgaatattcg cattttttcc atggattttg tctgcaaccc agtcagtttc acataagact
     1981 tctggatgag agtaagaggt aatatttgac ataacaatac tatctttctc tagtacttaa
     2041 tattttgtcg acgcgatggt cggctgattt actcttcgtt ttccaaacga tctggctgac
     2101 tatttcagtt cggtcttatt aaagaatcaa atatataggt aagcaacaat gtttagacga
     2161 atttgtttca cgattgaatc tttggttgta ataagaaagg ttgtaataat aaattatcca
     2221 aaagtgttta cctaaaccgg tcaatctgca attaccgtta atgattgtcg cgagcatttt
     2281 tagtagtgaa tacgtcagag ttaaatcata gcgataattt cctcaatttt ttttagcact
     2341 cactgtaaag catttcaaat atatcataag ttatcaaagt gaaatgtgaa ccacacgact
     2401 ttagcaatag ttattgtctt aacggctgca ctagttacag gagcgtcgtt agcgatacct
     2461 ttacaagatg caaaagcaag cagcaaccat cataaaaaac aaggcaacag cgtaaatatc
     2521 aaaaacaacg agaaccaagc caatgcatgt accggcgcct tggcatactg cgtgaacctc
     2581 cttagaaacg tagactgcgt acactcgatc tgcatcattg gagacataag cccatgggta
     2641 atggctacac cgcactagag tgatctagac cttccaaacc ttttgttctc gaggaagaat
     2701 caagatcatt atctctactt ttttattaca tctacttaaa tccaatgaac ctgaaatcaa
     2761 ttcattttct ggacatattt ttgtgcttct caattttaat aaccttataa cctgaatgat
     2821 aacttagtag aaggtggttc ttcctttact ctggtctatt aaaccgacga ccaatttttt
     2881 attacatcta cttaaatccg acgtacatga aaccaattta attgaataaa ttatagtatt
     2941 cctaccaaac cacaaattat gtggcaaatt tggttctcat gcataataat gctcagtaag
     3001 atttttagta ttagcttcta tattattgta gaatgacatt aagatccgtg agttactcaa
     3061 agtgccttag agcgctctcc atagaagcag gaggagctag agattgaggc ttcctggttg
     3121 gatatgcgtc acatgtgaaa agccatttcc aagaaaatgg aatgctgaac gccatgttta
     3181 tacgcaacat ccaaacgaat cggcccaaat tgttagcttg gtcgactatc tggtaggcag
     3241 gctctcaggg gtttatatgg cgagctctcg aatgcgagca ccaccgccta accttggtca
     3301 agttgaacca agcaccaaca ttttcgattt tttgcagcat gtaagtcctt tacatgcggc
     3361 cgagtataca aaacg
//
LOCUS       DAOR01000189           27609 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17086, whole genome shotgun sequence.
ACCESSION   DAOR01000189 DAOR01000000
VERSION     DAOR01000189.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 27609)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27609)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27609
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17086"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    9953..10198
                     /estimated_length=246
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10453..10656
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10875..11096
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25217..25252
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26554..26779
                     /estimated_length=226
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8376 a         4884 c         5147 g         8268 t
ORIGIN      
        1 ttgaaactgt aagaaaatgt ccatcaggtg ctcttagtta ttccgttgat ggagttgaat
       61 acagggatca agatagagag ccaatgctta cagtctcaaa agatggtcct tatatcataa
      121 ctggcggtat agaattaatg ggtgattcgc aatttggaga gggggcgtcg aaagaacact
      181 acacactttg ccgctgtggt gcttctaaca acaaaccatt ttgtgatggt atgcacaggg
      241 atatcaattt caaggatgaa aaaaactgaa gatctgactc atctcaatcc ctatcctata
      301 ctggctgacg aatcatctac ctttaatcat gattgaaaga cacggaggga aattagtatt
      361 aaacctagaa aaaagggtaa ccaatttaaa aaatcgagta gggttgcggc agtatccaac
      421 atggtctaca atctacttgt tcacaggcaa gcctttcatt acagaaacac accaatcctg
      481 gagcgactgc gagaagaatt gattaaacct tcttctttaa gattgctata tagtggtttg
      541 aaggggctgc tataggtttt ctgttggatc ggccagatgg tgccgtcgct ggttgtgttc
      601 ttggttccgt agtgcatcac ttgggaaatg gtattgctca gcgtttgtta agcaggcgag
      661 aagagatacg gctagggtgc agttattagt tatgtaagca ttagcattaa ggaaaaattc
      721 gactaggggt tgcaaatacg cgaagacgat tttttacaaa tcacgaacac cagcgtccaa
      781 ctgctcaaga aatagcagat ggaattctgc tgggagctca caggggcttc actttctatg
      841 gcttgctgtt ggcgaatata gctttttctt aaagataaag tttttccaga aatatgatac
      901 ttggtgaacg cttgtggcaa accaagacga tattgttaat tcaatgttaa ttcacctaag
      961 caagagaaac aatttgaaaa atgcaggaga aattgctttc ggagaaaaat atgtattatc
     1021 ttcatggaaa ggaaaaatga accagaaata acgttagatt ttgagtggat tgcttgcgcc
     1081 catcttaagc tccaaatgaa ctatatccag ggtacaacaa aaagatgaaa gatttggtat
     1141 ggaacaaagg gtgacagatg cctgttatag atgataatga tacgtatcat agaatccagc
     1201 cagtatacgc caccatcaat cagatatcaa ccttagcagg tccgaataag cttctcttgt
     1261 gatctcgtct tttgctaatc gttgtgatct cgtcttttcc ttcttcaatc aaattgtatc
     1321 gaataggtat tcttctattt attcctatta gcaagccgaa acaaatcagt aataacaaat
     1381 caaatactat gatcacaagt actgttgttg ataccttccc aataaccaaa ggcctcagtg
     1441 ttatatgaat aaataacttt gcgtatgtgt tttactgagt atagactaca agctctttag
     1501 acaggttgag tctgctcaag gatggtcaac ctgtatttct ctctccggct tcatctcctc
     1561 cttaacttaa tggtcctgga taggattaat cgctgacagc aacttttatc attttggctt
     1621 gttcaatatc aatcggtgcg tcagtagaat ctgttcttat tgcagctatt ttatcaacaa
     1681 tatccattcc agatatcacc tttccaaaga cggtatattt tccgtctaag aattttgaat
     1741 ctttcaatat aatgaagaac tgtgaacctg cactatccgg atcctgtgat cttgccatag
     1801 atattatacc acgtgaatgt gtatttttat taaactctgc ttttacgttc caaccaggac
     1861 ctcctgttcc ccatttacct ttgttggatg tatcttttgt atggggatct ccaccttgta
     1921 tcacgaagcc tggtacaatt ctatgaaata tcaatccatc atagaagccg ctctttgcca
     1981 atttcttaaa gttttcaacg gtctttggcg catcatctga ataaaactcc actgatatat
     2041 ccccaaactc tgtttttata gttcccatgt gtaccataat agcaaaaccg ctgtcaatcg
     2101 cctatatata tgcacacaga aatatttcaa tggcgtctca gtctataatc taatccgcct
     2161 aagtctcctc ggcgctacta acaaccgtaa aaggacgaga ggcagtagag ctaatactcc
     2221 gttcgttgca tattatttat gaattgcagt aactgactgc tggtgtaata attttggatc
     2281 cgccattgta ttcttttcag ttgctcgcaa tttgctctta tattttagat tgcgtggttt
     2341 agttcttaat ctataaccgc agcatggaca ccataacccg tcccatttta agaagatctc
     2401 acatatctga catcgtttct gtccagtaga ataacgccca gcaccaactg gtttgtgtgc
     2461 cttatgacgc atacatatac ctttacaagt catatgattc ctttcctctc tccctcttct
     2521 cttcggtgtt cataatcgag agacatttgt tatacttatc tgatgcagta gttaaagata
     2581 atggctcatt tgcatatcct tattatgtgg aaaatgatcg tcgttgtatc atagtagagg
     2641 aataagacga actttgttat cgttgggttc catacatttt ccgccttgac caactcataa
     2701 actgttggcc gcgtactcgt tgagggttta ttcaggccaa gtataacgat ttactcttgc
     2761 tagtaaatct ttgagttcct gtctttcgcc cggcgcatag tacgggccaa ctttccatat
     2821 catagaaacc aaagttccat cttcaggggt gcggtatgca agtcacaggt gaattgactg
     2881 actgactgga atatattctg gggaagcagt gtgacgaatg tcacatctga gaacggaaca
     2941 cagaagaaaa tcgtcatcga acatgacaag atgggcaatg tcataggaat ggatgctgcg
     3001 acaggaaagg aactatgata gaaaacagta ggtaagcaat atcatactga taccatccca
     3061 caaccgactg gtagcggtgt cgtttggacc gatggtatag atgactacta tgcagtcgat
     3121 aataacacac tgtatgttac gacaaatgac agagggataa acttctttac aaacggaatc
     3181 gttggttata aaatacctag gcctaatgca attgaattag gctatcacaa tggaacaata
     3241 actgccattg atatgggaac aggaaaaact aaatgggcac ataaagtgga ttttcggcca
     3301 ctagtctcac cattggtcac taatggaata gtattctctg gttacatcct tttcgccgaa
     3361 aaaccaagta tcaaatacac tattaccccg tttggaattc caaaaaaaat aacagcaacg
     3421 catcaattaa gaactggctt aattttggca ctggataagg tcacaggaca acaactgggg
     3481 aagtttaatg tagccggtcc gatagggaag gggaccttct ataggagatg caatgctcta
     3541 tgttcctaca ggtaaaatcc agtagcgttc ggtttatcgt aagctgagaa taacaacata
     3601 ttctagagca tggattttag gtcatggtat gatgtaaagt tctgtagatc ctgcggggcc
     3661 gcaattgcgt atgaggatag taaaaactgc ccggaggaca ttcatccaac tcccctagct
     3721 ggatgaaatt gggatgcact tgcatacttc actatctgag tcggcatggc tgtagctccc
     3781 tgtctaagga acgttggact aagcaacaaa aacaggcagt cctctttttc ctctcagtgt
     3841 gtatgtaccg gttgcagctc taaagctgac tatgaccagt tactaactta cattgattat
     3901 tttctgatat tgcagaaatc tcagctttca atcctatttt agtcggttac agacttcatt
     3961 aacagcaatt taattaacaa tatcaagttg ttgaaaccta aagctcggtt tgtatgtaat
     4021 aatccccaaa tgaaactgaa tattaggtaa caaaactggt cttgacttag ttttctgcaa
     4081 agttatttgc atttgctgca gcgaacttga catagtttgt cagatgtata caaggtacgg
     4141 cgtaccctcg cgggagggcg gcagcgttaa ttttacattt ttttacaaaa agttttaaaa
     4201 agaaatactt tctacgagca cgtgaatgag gcctcttggg tcttagtgaa cattgttcta
     4261 aaaactaaag ttgacctccc cggtagacca ggtaggaaat aaaatgacgt attaatctta
     4321 atatgttttg atccagttcc cgggaggaag gccgatgaca tgtcaaacac aaactcaaat
     4381 tccttggcag ggaacacttc gaaatcctgt tgcttgtttt aatcgaatga atgatctctt
     4441 ttcgtcctat gctcgctcat cgtttcctat tcggttttaa tctattccca aattcttctc
     4501 gagtaggtag catagcttac ctagatggtc taaatctata tgttttacac gttcgatatc
     4561 agccgaaaaa gattggtcgt agtagtggca ttcaaatcca agtacctcgg ccactcggct
     4621 tggctcagaa aatgtgaaat ggcggttccg tgctttcgaa taatttattt tgttcctcag
     4681 ttcacctgat gtattttcaa tatcgtttcg tgatatcttt tgtgattcgt tagcgatttc
     4741 tcgtttctga agataatagg caggcgaccc cactttcagc aaaattattt tttcgtgttt
     4801 ttcgttcaat tcattctggc atgactgacc ctgtgctgct gagccatggc caccgcttga
     4861 ttcagattca tgccgatttt caacagcagt agtattgggt gtagccttat ttgtcgtatt
     4921 agctttatag ataaggagaa accttaagct aattactata tggtgtcttt ataaaatatt
     4981 tcaatataaa cgtgttttta cgtacttttt acatattcat cttctttctc ttatggttca
     5041 ctataacaat atctcaaata cttaatatta tgttcgttta ttatgttagg cattattgga
     5101 agaactagat ttccttcatg aagtatgctt gactgcattg ttttccaata gagtcgtgaa
     5161 gtttgcagct gttcttgata acaatggaaa actacttgtc ggtgaatata ggaaaggcat
     5221 tcaaagttcc tggcaaggag atttcatatc tgacaataat tatcatcatg acccaagtta
     5281 tcttttctat ctggattacc tggttcctgc tataaagaaa agaagacttt tttgcacagg
     5341 ttatacagaa gaacaacaac gacagcaaca agaaattcat tttgaaataa cagaaataga
     5401 tgataacgta aggttagcta tggctccgct aacagaaaga aatgacaagt atctttgtat
     5461 atttttagaa tcgtcagctc cttatcaaga gatactctca aaactcagaa atgccatcat
     5521 ctaatgatca gctttactat ctgcttttcc accatagact aaactccggc taactttgct
     5581 ctaccataaa ttactgcttg atgtatattg ctaatttcat ctattttgtg ccttcttctt
     5641 tcgacagtat agccagtttt ggaatgataa aaaattacca tatctattgg ataaaacgaa
     5701 agcatatcgt gattggcata atctgatatc atatgcttta gatgaatcct ttcccatgta
     5761 aacaagtcat ctattttgcc gtcaaaaaag tgaaaggtac ttccacaagg acattctaca
     5821 tctctccaat caccagggat atttactaga cagttattgc tttcagaata attaataatt
     5881 ttgttgtcct tcttcagtgt attagataag atggacttta ttattaatag tttctaattt
     5941 tactggttga tcaaatgcta cagatagtta accaactaac taggatgcta cttggctaat
     6001 cttattcttt caatactgca cttagcctgt ttaataacct ggccgtgtaa gtgcgtacac
     6061 gaaaataatt tgggaggagg gtcaagtttt cttaatcgag aagtcctacc tgttcttccc
     6121 cataatttgt ggcttcattt aggtttgatt caaatttctc atagctattt cacttttgta
     6181 atctgaaacc tcggaaaccc cttttaatta tctggctact gtcttgttct cttagaatac
     6241 aaatcctcga tgtacacgac tatagttttc cattagaaaa tatcagctca atctaagcat
     6301 tagtgttaaa cactttcttc tagcgtcctc ctttcttttt cttagaacta taatctaaaa
     6361 ttgcatgtca aattagttca aaaaaggact gatcttgaat gtgatggaga tagtcgagat
     6421 tgtggaggta tttaggttat attatctgag gtacgctgtc aacaagtttt atagaaccca
     6481 caagaattgt aaatctgctg tgaaatgtta aggatagctt atagaaataa tctaaagata
     6541 tgagagcgtg caaaaattag tactatatgc aaatcggtaa aggatgtcaa ctttattgtg
     6601 aattgagatt ttcttgaatg gaaaaattgg atgtttagat tttttggttg ttgctgcaat
     6661 cctatgattc tttttcctgc gatatatcaa agcaactaca tcttctctat aggatgtatt
     6721 gtcgcgagtg attattacta ttactactat tgatttctgg ctggtccttc atatctcccc
     6781 cgcatttgca gctctctata ctatgttcgt caggcaggtg catctataag tgtcaggtga
     6841 tggagagctt ttcttcattt tgcaattgcg cgatcacgtt ttctaaattc acactctctg
     6901 gtttcagttt gcctatcatt ttttctaatt cttccctgac aagtgaaaaa catccccagc
     6961 tgatatagtt gggatatgaa attgctcgat gaagcgtgtc gcaagacata gtatatattt
     7021 tatctaaagc ttcatattgt ttctgcttcg gaaaaagttc attaattata taaaatacat
     7081 cttctgtttt gagtttagaa gtgaatctta tgtttacatc attagtttga gatttcattc
     7141 taatatgctg tcttatttta tctccaattt tagtaataat aagactctct agtccaattc
     7201 tcacagcaga aagacctagt atactttctg acgggttgac tagaaggcaa ttttcagttt
     7261 ctctaattat ctcatcgatt ctttcagata cctgaatttc tggatcttga acaggtaaag
     7321 ctttgaaatc tttttctctt ttagaaatat tggagtttat tttttggaat aaatcaaagt
     7381 aatcacgaat cgcagttcct atattttgac atattccgtg aatttgtcca tatacttcgt
     7441 tgattatgtg aagcagatat cgttcatctt ttgtgttaga attatccttt tcctcatgtt
     7501 ttttccacag gaatttgaaa cgattatgct tatttataag aatgttcaca agcaaaatga
     7561 cagttttctg acagagtatt aattttgaca tccaataatt aatatattct gttcgtgtcg
     7621 ttaatgagga ttcattaacc atgcctttta gaatgaattc tgcactcgca gaacggagta
     7681 gcctagtcct tggtttactc ccagccgaga gttctattaa ttccttaatg tcattttcaa
     7741 accttacttc taaaaattta agcctagaag atatatcctc agtacttttt ccgaccatat
     7801 cttcaaaagc agacaacgag agtcgattaa aaaattcagc cacatctata tcatttcgca
     7861 tagattatga caacaccaga aaataaaaaa ccttttgtgt atatggagaa ctaatgagga
     7921 tttataagaa catatcaaca tgtgatagtt aaaatatact gtcgtgattt tcaagcctaa
     7981 agaattcata tacaggagga gagaatttac ttgattcaag attctacgat actaactgct
     8041 gaccaccaat tcgaacaatt tttagaaccg taaaatgaca aagaaatagt cccgaagatc
     8101 agattttatt gaatgaaccc ttttgctatt attcttgttc caacattatc tggctttaaa
     8161 agagcataat tctggccaga gaagaacaca aatgatttct cgggaattat ttgaattgta
     8221 ccggtattat tatattttat ctttactggc ttgatttgta ttccaacaga tataacgtat
     8281 gtctggttgt ctattagatc ccctttgtaa taaggactct ttataaatcc tgcaggaata
     8341 gcatctgttg ctacctttag atttaatgaa ccaggagaac aaaggacgtc tcccctagaa
     8401 atatcatcag catcaacccc tttaacagcc agccctactc ttgctggact tttgctttcg
     8461 gttacaggat catcatgcat ttgaattgat ttgacaagta tgtcttttcc actaggtaaa
     8521 attttcaaat gatcaaatgt cttcacagca ccctgtttta tgacacctag tatagtactg
     8581 ttccaactcc ctttacgtta aaagcatggt ctattggaat tatgctctgt ccttctaggc
     8641 tttttggttc aagtcgactc atttgttgtt ttaattggtc aatattatca agaaattgga
     8701 agtctgagat ttctgtgttt tttattaatg tttttagctt catttcatct atctcatacg
     8761 aatgcaggat gaatccttca ctgaagttga ctgcatccaa agcgaggact tgctctacta
     8821 gatacttgtc tatttttgta acattcagta tagcatattc agcaatatta gttgcctgca
     8881 tcaaagattg aattttatcg ggaaaagtga cgggcacagt ccaagtgtaa atagtatctt
     8941 gtgtgtttct gtcataaatt gataaatcag ttgtggtccc tttcttacct aactcacttg
     9001 caatttcata atcgccaact ataacaaaat ttatgctctt catcagagta tgcctaatac
     9061 atggcaataa aatgtttcct aaaccgcgta acttcccttg catctgatgt tttatgtttc
     9121 tatactagta taagttgcgg tgcgcgatag aagaataagt agtggtcgta gtgacaaaat
     9181 tttatttgtc tacctggtac aatgtcttac cctgtcttga gtacagtgtc tgattacttg
     9241 agtactatct ttgtactcta gtacaaataa gcgggaatga atatgacaga gacaagtatt
     9301 gaaaatatca gaggaaataa aaagtcaaat caacaaaatt acaccaacaa gcattcggtc
     9361 gccctcatac tcaactagca ttatattatc gaacctcgca atcttcctct ccgaaatacg
     9421 gatctgtgat gattatgaaa agtataacaa aatgaaccga cccagaaata gactattgaa
     9481 aatatgatgt tggagttaga tagacctcat gttcattgag tttttgtaat aaatctgttg
     9541 ttccactacc tgagagaatg gcatcttgtt cattatctag cgaagtattt ttcagatgtt
     9601 ctttaggcga gttcaagaac aaataattat gcattaaaca gcgcgttcgt ttcaagtaca
     9661 gattcttcta ctgaaattga gaataatgag atatttccag caaacatcag gcatgatctg
     9721 agaaattctg gggtacagca aacaagattt agatataccc tttaaacctg acgcagtcgt
     9781 ctgtagaaat tactttgaag gttgcttgag ggaaagacgg attagagaaa gatgttgcca
     9841 caggtgtgac tgaagcagtg ttcaacagtc taccgccaca gcgtattgat atcttttgaa
     9901 atatacagac aaagaaaaag tcagtggttg aattaatagt actatctagt acnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
    10201 atttatgtca acaatattat atgttgtctg aattgggtgt tataaaatca gaatgttaac
    10261 ttctataact ttttgcatgc gaggcagaac aatacccgca gtaaccgata gtacctgttc
    10321 ttttatgaca tatgcattca catctagagc caaatcctga tgaaaacttt cttctcatac
    10381 aattaattgt tattcttaca catatttaaa ttaccctccg acaatagtct acaatacttg
    10441 acaagaaaga atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggg tttttggaga attagaatta
    10681 ttggtaaaga cggagaaaaa ttaattgtat acgattttaa agaattgaaa tgatgagaag
    10741 ttaaggaatt gattctgcta gtcctcctac cacgcctgtt gctcctcata ttggctgcaa
    10801 tcccttctcc ttcaacacct cattcagaat tgtaagatcg tatatccctc acaggtctgg
    10861 tcttggtttt ccctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttta
    11101 gtggatcata tgtgaattgt attcttgacc aagcttgcct caactcattt tctagtatgg
    11161 ttttacctgt tagtttttga aattcagaat tgaatgcctt tatcgtcaca tctgtatgat
    11221 tattaatcca ttatgattca ttaacatttg ctgcaactag tttcttgatt agatcaggat
    11281 tttgttgcaa gtaatctgtt ctcgccagta tgctagctgt tacgaactta ccgtctgatg
    11341 gccacaaggt tctttcatct aacaacagtt ttctaccaac ttcgttgacc agtctcgcat
    11401 cccaaggatc tgatacccat tcgccctcaa cctgtttctt taaaaataat gtaggaataa
    11461 ttgttttatc agtggtacat ctcctccgtt gtctttcgtc ttatatccgt tatcttgcaa
    11521 atactatcga agggctgtgt atgtctgttt ccgagttgag gtgaagcctt aggagacttg
    11581 attccggaat cacttctcac tacaaagacg gtagcgccac ttgccacacc ggaaattatt
    11641 cttcgatcct ttccctgaga tgcccatacc catttactgc tgagcctggg ccaacgtacg
    11701 tgcgtctatt ctggaaattt ccacttccaa acccgatgac ggtttgagca tggtttatgt
    11761 taggaaatac cctattctca atacctctgt tgcacttaag cctgtacaaa tcctatagca
    11821 ggtgtatacg caatagctgg cgcaagtatt attgcaataa agggtattgt taattgcctt
    11881 cctatcttcg aatgtatggg ttctaaagca ttttcacatg aataaggaat aaatttcctg
    11941 gtacgtgtta gaataacgga ataaatgtaa atatagtata tgccaaatat gcgccattct
    12001 agtcctaatt gcgtcttcga agaaagttat attgcataac cgcacttaga attaccaaga
    12061 atgtctgttg tgtcacaatc aggagaacta gaaagtatcc tgcaagtcaa aaatgtttcg
    12121 aaggtctaca gtaaaaatac caccgcgtca gaccacctcg tattgaataa tttgaacata
    12181 gatatcaaag agggtgaatt tttagatatc aaagaccatc aggctgtggt aaaacatttt
    12241 ttgatctttc catggattac agttcttgaa aatattgttt cgccaacttt cggggggaat
    12301 gaaacaatgt gcagccattg gtcatgctct ggccatgaat ccaaaagtct tgctcacgga
    12361 tgagcctttt tcagcactag acattcaatt acatcaatac agaacagttt tgtttctaac
    12421 gcacaacatc aatgatgcta tggtattagg aggtatgatc gttgttttgt ccccaaaact
    12481 tgctggtata aagagagagt gcactgtagg tttgccacga ccaccagatc atcctatcgt
    12541 ggattcaaat gcaatggcga ttaccgaaga atccaaggaa ttatattttg atgatgaaac
    12601 gagagaggat aacaaaattg cagccacgaa agtactccat tgaagagaaa ataagaagaa
    12661 aaaaaattag taggattaac cttaacgata tagccaacac aatactgttc cttgcggtgt
    12721 ttgtagggat ttggcaacta acatttatgc tgggaatatg gcccagagtt gcattgccat
    12781 caccagcaat ggttgcccaa tcttttgctg aattgaattt ctcattaatt attaacatca
    12841 gtataacaat gtggcgtctt atgattggat tctcaatttc gatcatttta ggagttggga
    12901 ttggattggc tatggtaaag ttcaaagact ttggaaaaac gatgagttcc tttgcggtgg
    12961 gactacaatc attcccaagc atagcctggg taccgttcgc aatactgttc attggtctaa
    13021 acgatttcgg tatcttattt gttgtaatca tgagttctat attctcagta atgttatcta
    13081 catacagtgg aattcgcgcc gtcccaccaa tttatgctcg tgcagctaga aatttaggcg
    13141 ctaagggtat gccgctattt aggtatgtga tgatacctgc ggctacacca tcattaatag
    13201 ttggagtaac acaggcttgg tcatttgcat ggcatgcgtt gataggagct gagatattga
    13261 tggcagcata tctaggcttg ggtcatatcc tggacgttgg gagagaattc caactcatgg
    13321 atcaaataat tgcatcgatg atcactatct tcgcaattgg tctggtattt gaccgacttg
    13381 ttttttccaa actagcaaat agagtaagag atcgatgggg actgaatcaa agcaatgaat
    13441 aaagataatg acaactgttc agataaggac gtaaaactgg ttcagattag aattgtaaat
    13501 aatagactct atcgtagtag aaggcggcat ttgcagagca ttatatgata gaaagtccat
    13561 atgatacggt ataccttctt gcttaggaag ctagttagag gatctaattg acaaactttc
    13621 aaattatatg acacatcaag agaaaatacc aatgagaagt tatgcaaatg ggtgaatatt
    13681 tcatcggctg ttcaggttgg aattatccgg acactcctga taaaggtgga tggactgaca
    13741 tattttaccc agataaaaac acaaaaagac ttcgatacta ctctcaattc tttaatactg
    13801 cagagatgga ttctactttc tataacagat tctactccaa aatgacaaaa ggtactttta
    13861 ttggaatcgt gaaagctact ccagagaaat ttcaattttc aatcaaagta cctgaaacaa
    13921 taacgcatga taaaagacta gacatcgaca aaggtgctat tacagatttt gaagagtttc
    13981 ttgacaaaat ctcaccacta aagacagcca acaaattagc tgcaatactt attcaacttc
    14041 caccaagttt tacagttaat gattttaaga atatcgaaag ttttctagac aggctgccga
    14101 aaggtaataa tggaagaagt aattatgatt atgctatcga attcagacat ccctcgtgga
    14161 agactgaagg accttgggag atgttaaagc actacaatat tgcagctgta ataacagatt
    14221 caccatcaaa agaaaatttg caatacttgt ctgatgttat agttacaaca gaaaatcatt
    14281 cttttatgag gtttcacggc agaaacacca aaagtcatta ttggtatgac tacctttatt
    14341 tcaaagaaga gctaaaacca tgggtcgaaa aagtctcaca gattagaaaa caaacaaaag
    14401 ttctcagagt gtattttaac agccattgct gaggaaatgc tgttgtaaat gcattacagt
    14461 ttagagaaat gactggaaca cagttgtctg acggtgaaac aagagcagtg aaacatgcag
    14521 agacatattt ttcaaaacaa caccagcaaa aaaggctaaa ctaacccaaa agacgataat
    14581 cggtgagatg aggatccaaa agaaatgagt agcctttttg tttaatctaa cttttgtgag
    14641 tttggaggta atgactccaa cattccacga gcaggagtgg aattagatcc tgttagaatt
    14701 tcagataatg gtgtttaaaa acagctagta aagtaagtga aaagggaaag atgacaatat
    14761 atggagttag ttagatatta ctactgaatt caaatgagaa taatcctgtc gcgtcttcat
    14821 gaatgatgaa gcaagaacaa ttttagaaac tgctgctata ggaagcattg gatgtgatct
    14881 tgtagagcag ctgcaacggt gttaaagccc taagactgac tgaacactcg aagacattag
    14941 gagttcaaga aacagatcac acaccagcta tctaataaca aaccattttt taggccaata
    15001 ccttatatat ttaaagagcc ctcatttagt catctcgtta tcggcaatat gtgctgttta
    15061 gacattaaac acgtctcgtc cgtccactat atgcatcatt tctttttgaa atattccttc
    15121 ataggttccc aatagtaatc aatccatcct tgtctatagg attctacttg ttcggcagga
    15181 actttagaat gagtcattat taattcagta ccctcttctt ttgaaatgaa tgataattcc
    15241 aagattgatg gtggataacc tttaggccat tcagttgttg tccattcttg cattattttc
    15301 tttccttttc tcaattccaa attttttcca aagatgtaac catcccatgc tgtaaaattt
    15361 cctcctactt ttggattgca agttgccttt ccacctgtaa agtccgaatg tttttttgca
    15421 tttatgtagg catcgtaaac ctgaacaggt tttgcaggaa tgaacaccgt ctgtttaata
    15481 accttgatat tttctgacaa ctcgattcat agttgattgg cgtaaatgta tttttaagac
    15541 tttggatgat tctggctaca attgtatgta attccgcgaa aataagaata agaaaaaagt
    15601 gacatgaaac gctaagattg taagtgaccc ggcaaagaaa atcgatctgc aaggaccatt
    15661 gactggcaat atgacccctt caggtgggat ttgatgtcta tcgttggttc tggtgtctct
    15721 gatgaatgtt gaatttccac ttagtttact ttcttttgag gctacccttt cgatttcgga
    15781 tggccaatgt ttacatcaat tgatggtttt tgaagaggat tatattactc gctcatgcaa
    15841 acctttaatt tttaaaagct tgaaccaaaa atattacaaa cctgatcatc ctccgaatgt
    15901 cggcttagca agaactagga ataccactca actaccatac gaaagagttt caggatcttc
    15961 tgacgcgggg gaagtaatta gcagtaatta agaaaaagcc aaaactactt tttcttacat
    16021 ttggattggg aatatctgat gaatatatat aatattatcc tgagaatact cgactgctat
    16081 acaactattg tccatataag cagggtatag aaaggaaaac aaattcacgt aaatatcgta
    16141 tgaaatttca gaatactcac ttaagctggg ttcatatgct ctgaattttc tactattcta
    16201 ttattagtct gtctctctat tttctccaag ctctctatca agctgatttt ttaatgacgt
    16261 gtatctagta aacttcttgt cccagttaga aaagaatctt tgttcactta atccaatata
    16321 caaccacatt gaagccaaca ttattgaaac tccaataaaa aacaggagaa aaaaacctac
    16381 ttcggggatt ctaaacaaga ttctattcat aaaaaaagga tgaattacga agactgcagc
    16441 caacattaaa gacaagggtg caagaataaa tgaagatagt atcattacct taaatacact
    16501 tcttgtcgta ttcatatgag atattagttc atctagtaac tcaacgacac tagtcttatt
    16561 ttgatccttt atagaattat ttgaatcatt gtcattacca tctgaagaag ccaattctat
    16621 agagttctgt gatctaacgc ctcttaaaat acttggctaa ccatgttact catctcacaa
    16681 gaatcacttt tctttttagg aaacatttgc tcgtatggct ccaaaattct tatgcaaaag
    16741 tcttttctgt aacactatat tttactattt tcttattacc ccatggctct tctcttcttt
    16801 taataattcc tttttgttcc atgtcttata gtatttcgtt atgcttcgca aggttaagtc
    16861 cagtgttgct taatagagta gtctggttaa gctcaccgta ctcggttagc ttcaacacga
    16921 tatcctttct aatgtaaatg cggtccctgt attcatgcga caattttatt gcgtcatgct
    16981 gaatagcttt gtaagtaata cggcagacct taaacgtttt aagaagcatc tctggaggag
    17041 gcttggaacc ttccaatgga gttaggtcaa gggcccaaga tgatatactt atcgctagtt
    17101 tgcaaatttc gcctgaaaaa cacaggaagt aagccatgct tcacttgaac aatttttagt
    17161 ttcatttaat tatgtgtgtc tatcgtagat acctgataga acgattttgc ataatactga
    17221 gaaactgaaa gtgactttat cttaaggaga aaagagtgaa tgccaaaaca gttacaattt
    17281 ttaccattac agtgatgtga taacatcaag tctaacattt gctgcaaaca ataaaaacca
    17341 gccaggacaa cgtatcacaa taagcagagg gaagatggca aatggcgcta tatcggtgat
    17401 acatctgtaa ccatccaagg tcttccagga ctgacagagc caagttctat aaataataac
    17461 tttcattgtc gattctcaat atcaatctcg ggaataggta cgggtactgc gatagcgagg
    17521 ttcgatgtgg tgcgcgattc cttgataatc accggtcttc catgggaaac acattcaggg
    17581 tttgtaactt ttgactcatt tggtcccgat ccaagttcgt caaaaatcga cgtccccgga
    17641 aaagatacgg gtactgcgat aataaagcaa aacttggcca attaattacc tcgaattgat
    17701 ggactcgatc tcgcattata agttactcct ttatggtatc attgcaagga acaggattat
    17761 agttgcgtct ggtacctatg tttctaacac tccggtagga cgtaagataa ctgatattaa
    17821 cttcaataat agaacgggag cgggtggaag aattcgacct tgacttgacc gatatttcag
    17881 attttggatt tcgatcagcg gagtgtcaag ctgagatcat ataacgggga atcagaagaa
    17941 gaaatgattc agttaagcac tacatattct caaaatctta tcccttccag ctaataatct
    18001 tggatttaat tcaatgcgta tgaacatatt attccatttt atcgtgatga tgtaaaatca
    18061 gtagatcgca tcatttttcc tccaggatct ccaattccac caacaataga tgagaatcca
    18121 accataccaa acgatgaatc gtgaactggt gcccgatgca atttataaat ataaagagag
    18181 atttgtagac gtattggtaa tagctataca gagagggtcg aggaaattta gttgacagct
    18241 tataatttga agttgataaa cacctgacta gatggagtaa aagtatatac attgacttga
    18301 caaatagtat agtatataca gagaatatcc tggttaacat ttagatatcc tcggaaatgt
    18361 tccctcgctt ttgtggccat tatgatgcga tgataccaaa agaggaacct gaagtaaagg
    18421 gatgataatt aaaagcgaga gattaaaacg tgcgatcctt gctgctttgg ccgacaccga
    18481 attacagaag attttggatg cagtcatgta ccagtctaaa tcagttaatc aaattatacg
    18541 cgaaacggat gtttcccaca ctactgctta tagaaagatg agatggctcg ttgaagagaa
    18601 gttgcttata gttgataaaa ttgaaataac ggaggacggg aagaaatcta gtttgtttcg
    18661 tacagtacta aagtcattta atgtcagata tgaatacaat aatgtagtta tcgaggctga
    18721 acagaatata aacactttaa gaaaaattac agaaaagttc ttttcattag atgagtgaga
    18781 tgacccagat acataatagt caaaaaagga aaataagaag tgtctaagcc gccaacatca
    18841 tactaaaaaa agaccataga aaaacatgta ttcctgagat agagtaacgt aggacttcat
    18901 actgcaaccg tgtttgttat gatcataatc ctttgtttag ctctgccaaa atagatgatt
    18961 ccaaagaata ctaattctgt aacagaaagc gattcaaaga ctgtgtctgc taataaaatt
    19021 tggtcaaaaa atctaaccac ggatagacgg caggcacagg caggaatata aaaatagaag
    19081 gtattgcaag cgattttcaa atatggtcta tatcatatat tagaacactt catatattag
    19141 aaatgagaaa aatgctaaat ttactgttaa attacaataa aaaaccaatt atattacttc
    19201 tgtcgagtgt atttcagatt tgaaagtatc aatcatcgac ctgggtttta actccgctaa
    19261 attagtgaat tattatgtta atcgcaacaa caattcgttc aaggcttacg aacacgaagg
    19321 agcgaaagtt agacttggcc aaggtctcca ctactctggc atcctcggcc gcgagcccat
    19381 acaacgtaca attcatacct tgaaattatt ccgtgatata atcaattttc agcgcatcaa
    19441 gtccgtatta tctatcgcta cgagtgcggt aagggaagca aacaataaga atgattttct
    19501 tcatgaagtt cttcaaaaca ccgggtttcg ttttagagta ttatccggaa aagaagaagc
    19561 tctctactct tatctcggag cgcttcagtc aacatgcatc cccaccgctc tctttttcga
    19621 tttaggcggc ggaagtttgg aattagtata cacagaaaat ttcaagatca aaaaattaat
    19681 ttcattacag ttgggggcac taagaatgtc acagacctac agcgatgtcg gaggaaggtt
    19741 aagtaagaag aattattcca gaatggaaga acacataatt aatgctcttc ctgacagaaa
    19801 agggcttgat atcagccctg acacaacact cgtgggggta ggcggaacat tacgagcgat
    19861 ggcaagatac gatcaacaaa caaaaacgta cccgcttgat aaaattcaca attataggat
    19921 agactttgac catattcgct caatcaacag tacatttcgt aaattgactt catatgaaat
    19981 tgccaagatt gatgctgtgg gtagcaaccg agcagaaaca attacggccg gctcaggtat
    20041 aatgaaggta ctaatgcaaa agcttgaatt tgaatatata ttggtgagtg cacaggggct
    20101 tcgggaaggg gttctctctg ctttcttgca atgctctaaa ggctattact cacaaaatat
    20161 caatcaaaat caaattcaga gccatgttaa acattgctgc aaaccagaga aaattccaga
    20221 atatacgtat gccctagtca aaccattata ctcttcagga ttaatgaaag agcgagaata
    20281 tgagatcttg gctcatgcta ttaagcaggt ctcaggaatg cctcctacca caaatcttca
    20341 caatctgttt tatatgatta ttgacgatga taacccacac ctcagtcatc gagaacagct
    20401 gattttggcg ctatcaataa ttcgtacgaa gaaagctaag accgcaaatt ggcttattgc
    20461 aagatacagc tcaatcatgc agccgcagaa tagaagatct atcgaaaaaa ttgcagcctg
    20521 tctttccctc tcggaaattg tcgaaaggat taaatcgaaa gtcagatttg ttaaatgtag
    20581 ccaaaaggaa attgtattga ctttcatacg agcaaagaat aacgcttttc ctctaactct
    20641 gatagaaagt gccttgaaaa actttaagga agcgttcgat attcccgtca cttattctgt
    20701 ctctcatacc cctagcagag ttttagctaa gcagcaagat gttgatgagt gagtaagaga
    20761 gtcgaagaaa agatgaaaag agtgctgcaa agtaagaaat tggagacaaa aggaaaactg
    20821 gtcgtggttg aaggtatcga cggatcagga aaatctacac agattcgttt acttgaaaag
    20881 tggctaagat tcaggggtgt aaaagttttt ttgactgagt ggaactcatc agacttggtc
    20941 aaacaaatta catctaaagg caagaagaaa ggcacactca caccgacgac gttttgtttg
    21001 ctacatgcaa ccgactttgc ggatagatat gagaggaata tcttcccttt ggttcaggca
    21061 gggtatatag tattggcgga tagatatgta tacactgcct atactagaga tatagttaga
    21121 ggctgcgacc ccagatgggt tcgtgaagtt tatgactttg caataaagcc ggacctcgtc
    21181 ttttatttca gagtgcctat tgacgttgca agtgaacgaa tactagccgg caggcctaag
    21241 ttaaagtact atgaggctgg tatggatttg aacttaagta atgatattta cgaaagttac
    21301 aggatcttcc aaagccgaat tatcgatcag tatgaatcta tgatccagtc agaaaggttt
    21361 ttgcctatag acggtacctc cggtattgaa gaacaacaac aattagtaag aaaaaaaatt
    21421 gctgaaataa ttccgcaaga caaaggacca atacgtatag tcaaaaaaca ggtgttgaaa
    21481 gatgggtaat ttacgacaat tgagaccgac cagtgacagg cagatagagt attatggtaa
    21541 gggtctgccg tatttggacg aaacgaggat taaaggccga ctcattgtta tagaaggacc
    21601 cgacgcatca gggagaagta ctcaaattat gaggatcact tctagacttg aagcagacgg
    21661 tcatgctgtt ctaaatacgg gcctgaggag atcggaacta ataagtcaag ggatacttga
    21721 agccaaacga aattttgttt taggaaaaag aactattagc cttttttatg ctgctgattt
    21781 tgctgatcaa ctggagaata agataattcc tgcactgcgt tcaggttaca tagttctcgc
    21841 tgataggttt atctatacac tcatggcgag ggatgcagtt agaggaatta gcattaggtg
    21901 gtctcacaac ctatttggtt tctccattag accagatctt gtattttatc tagatgtaga
    21961 tcccaaggag ctagtacata gggttttcca aaaaaaagca tcgctagatt attacgagtc
    22021 aggagcagac cttgggcttt ctgataatat gtttgaatca ttcctgattt atcagcgctt
    22081 ggtcgcaagg gaatttcgca aaatgcaaaa caaatacggt cttgtgttca taaacggaaa
    22141 tagatctata gaagagattg gcacagaatt gcaaaaaaaa attgacgact ttgtgaattt
    22201 tcgcagttta gctgatacta gtgaattagg gcttattgga cgtaagcagg agttgaatca
    22261 aacataatga tactattaga ttcgtgcatt ctttctctat atggaagcta taatgccata
    22321 gtgtttttgc aggtaatcat gaaataggca tatccccatt ttcagattgt ataagagggg
    22381 ccttcatatt cttacaaaat cgagtagcga acaacgaaag aaagaattgt cttcattttt
    22441 tcttgatggc aaacgaagag aagataagat aatttatgcc ataacgatgg gcgccgccag
    22501 ttatacgctt ttgatgcttg ctctcgtcat ttactcagta gggtgagact ctgtaaatat
    22561 attttcgttt gaaggtctta cttttttgcc ggtacaagtt ggaattccgt cgaaggtcgc
    22621 gaatcgtgtg gtgccttgcc gcatattatc ggagctttgg ttagctcggc gatcgcgatg
    22681 gtcatcgggg ttcaggtcag cttagaaagt gctatatttc cgaaaataag taccccgtta
    22741 tcctttgtta tagaattgtt agtagtcccg agtacaagga tggtctatgg gcgctgctgg
    22801 tttttaggtt ctggatcgta ggtctaattg aaatgcctct ataaagcatt tggaggatca
    22861 attccatttt tttgtaaaag accgttccga attcgatccg ataagcaggt cattcattaa
    22921 gaattccaaa gatgaaaatt acgctattta tagcgcgcag aaagtggggt gctaacgggt
    22981 ataatattgg ctatatctag aatagctgga gaaacggctc ctcttatcat gacgatgctg
    23041 ggaacgacct tgttttttac tagcctgaca ggtcccgttg atgcacttcc tctgaggatt
    23101 tggaggctag catcacaacc ctatgaatct gctcatgtag gtgggggctg cccttgttct
    23161 gatactccta gtcctcagca tgagcgtgat tctgaggctg ctagcacaaa gaagaggatt
    23221 ttgaataaga acgacgatcg cagatgggta aaaagcgact atattgtcta tgttatctat
    23281 atatgcaaca aataattatc acgttggaga aatataaata gcatttgagt aacgaggagg
    23341 aacttgctag agtagtcttt agccagggct ctgagaatgc tttgaaggaa gaacaaaagg
    23401 agcaagctta caaggtctct attaagaagc ttgaagcatg gttcggaact aaacaagcga
    23461 taaaagacgt tagcttggat gttaaagaga atactattac cgctattata ggaccttcag
    23521 gttgtggtaa gactacactt attcgatgtc tgaatcgcat gcatgagatg actccgagct
    23581 cacgctcaag gggtcaagtt attattgacg gtattgatat ttatgaccga aatatagatc
    23641 ctgtgattat taaaagacgt attggaatgg tctttcaaaa gccaaatccg tttcctacca
    23701 tgagcattta tgataatgtt gcggcaggac taaagcttaa cggtgtcaaa gataggcaac
    23761 ttattgatga aattgttaaa gaaagtctcc aaggggctgg cctatgggat gaagtaaaaa
    23821 atgaactcga caagccgggc atggggttat ctggtggtca acaacagcgt ctgtgcattg
    23881 cgagggcact tgccatgcaa ccagaagtct tactaatgga cgaacctacg tccgcgttgg
    23941 acccaatggc atcttctaaa attgaagaac ttgttcatga tttgaaacaa gaattaacaa
    24001 taattattgt gacacataac atgcaacagg ccgcccgagt atctgactat actgctttta
    24061 tgtatcttgg ggagctagta gaatatggac caacgaagaa gatatttgag aatcctcaaa
    24121 aagagctcac agaacgctat atatctggta aatttggcta gaaaatctaa tttggttagg
    24181 catcattttt ttgactagac tgttggatct cggtattgac cgtatccgga atatcataat
    24241 ggatatggca aaattatctg agcaatcagt gttcacatcc attgactctt atgacaaagg
    24301 aaccagtgta aaaaaacaaa tatttgagtg gtcagaaaag cttcgtgttc tacaagatga
    24361 aacagccgac cttgcaattg aattaattgc acgctaccaa cctgtcgcca ccgatctgcg
    24421 gcttattagg tcctgcatgg aaattgcgta cgggttttct cgatttggac gctatgcata
    24481 tgacattgta gaggtacttg aaacaatagg tcatatcaat gactgcgata agactgctgt
    24541 tctggggatg gccagtactg tgagagaaat gatcttgctc agcgtggaag cacttcaatt
    24601 acgagacaaa aatgcatctg ccaaactcta tgaaatggat gatacagttg acgctctata
    24661 cagaaaatat ttgcgtgagg ttattactcc gcaaaacgat caagacaaaa aagacatgaa
    24721 tcctcgttgt tatatctctg ccttactaat attgaggtat ttggagcgaa tttcagacca
    24781 tgcctgctat attagcgact ccattcatta tatcatgaca ggaaggtcaa gtccaagacg
    24841 ttaactatat agtttattgt ttcatatata gcggtatatg attaattatc ataatatgtt
    24901 tatttaagca accttctaca agatgcttgt tgagtcgtct atctgaagaa aatgaagtac
    24961 gtaagataca gtttacaggt aaatctacat acatattgtc cttgcctaaa agatggatga
    25021 atgagatgca tcttaaacct ggtgatcctg tcacaataat tcgtgaatcc aataattcct
    25081 tgtccatagt ccctaatgcc gtaaggagtc ctagttcaaa taacgaagta actgcgctaa
    25141 tgctgcagga tgaaagcggc aattctttaa aacgtaaggt tgtgtcgatg tatttggcag
    25201 gatatagtat tatgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnataagaga
    25261 cagatataat attatgcact tgaaatcaaa gactggaaga attaattctt ctcaacgtga
    25321 tgcggtcaga gaagtggtgc gaagaaactt ggtaggaaca gaaataattg ctgatgcatc
    25381 tgatttaatc acggtccagg ttttgctcaa tttaccagaa ctttcagtca atactgctat
    25441 ccaaagaatg tttttgattt caactgctat gcataaagat gcaatggctt ctctggctga
    25501 acaaagttat gatctagcga gaacaatatt aaaatcagat gatgaagtag atagatttag
    25561 tctgtacata ctgcgaaacc tcgtaatggc aacaaagaac gagcggatgc ttcaagaaat
    25621 aggattaagg ggcccctctg acagtttgag ttatcgtgtt gcagtacgaa gtattgagcg
    25681 tgtggctgac cacgcttcgg gtattgcaga taaatgcctc aagataacgg agaaaatttc
    25741 aaaaactaga taagatgagt agaatatcgc tagcactact taatgactca gtcgaagctt
    25801 ttctacgaag agactattat ctggctgata gcattgtaga taaaacagaa ggcatacgtt
    25861 cgttggaaaa tgagataatt ttatttcttg acaaagaaaa gacttctacc tcaaccctgc
    25921 atgaatctac aaatgtttat atcaagctaa ttttagagga catacgaaga actgcggaac
    25981 atgcaagtga tatagcagaa gcggctatga atgagacggt gagtgaagta atcgaaaaaa
    26041 tacccagtcg tgagaggaag gtggttgctc aatgatccat ccgccatatt acataagttt
    26101 gtagagatga atgcactttt cgtaagatac taatcaatca aattaactaa taaccaatcc
    26161 ttgcatcagc attttgtcct gtagtagggg ctaaatagtc ttcgtagact aaataaatgc
    26221 tatgaaatag agttatgaca attcgacacg ttcttttatc ctgtcaagca ttgaatccag
    26281 cttctcagag ctaatctttg gtctgttctt aaacatcgaa tgaactggtc ccgctccgtc
    26341 accactgata cgtggaataa tatggatatg gacatgtaat atttcctgtc cggcttctaa
    26401 gccattatgt attgctatag ttgaagcctt gacacctgtt gcctcttcca ctgctggagt
    26461 tacaaccctt agcaaatcaa atagtgcaga tgaaaactcc ctattcatct cctgtatttt
    26521 agaataatgt ctttttggaa tgatcagagt gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26761 nnnnnnnnnn nnnnnnnnnt ctttttggaa tgatcagagt gtgtcctaca gataagggaa
    26821 aagcatctag aaatgctaca gctttatcat tctgactaat aattcttgca gaaatactac
    26881 catcgacaat tttgcaaaaa gtacaattct tttttaacgt tttcaacgac acttaaaaat
    26941 gatctgtgcc atatttataa aatactaata agaaaagctc ccaagaacaa attatttatc
    27001 tatggtagac acttgagtga aaaataagaa ggtataagga tcgtgtttat tccagagatt
    27061 taggtggtag tttgcgatct cctgactaag acagttctac tacggctaga aatttcatac
    27121 tctgataatg tctatgctgc tggcatcttt agatgattta gttgctctcg gctatatctg
    27181 taagacttct ttggtattga cagacagaat atgaaatgtc ttcatcaaaa cgcagttggc
    27241 tgcaatgaat tagaacagtc tgtcgcattt taacagcaat taatcccaag tggtttcctt
    27301 ttgttcctca ctacctgtac attctgtctt tactcgtgtg ctcccatctt tacataaacc
    27361 atgtttgcgt aacagcttga tgcacttacg agaatatcat aaaggtcata tctagtatga
    27421 gaatgctctc accagacgat aatatctcta ttctcaggtt ttaataattt ttatcaatta
    27481 atatcaacca ctggaaataa cgtctttaga ttcacctaat atagccatat ttgcactaat
    27541 tatccatctt atttcaatag tctttagaaa acgtacgata atgtaaaatt atcgtatggg
    27601 aaataatag
//
LOCUS       DAOR01000190            9778 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29304, whole genome shotgun sequence.
ACCESSION   DAOR01000190 DAOR01000000
VERSION     DAOR01000190.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29304"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2290..2558
                     /estimated_length=269
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3006 a         1713 c         1756 g         3034 t
ORIGIN      
        1 aatcgagact acaataagaa aaaccgatat tatgatactg gccatatgga tagaaatgcg
       61 gtaaacgaaa aatgggtggt taatatgtgg aagaatagct actggtaata atacagcagt
      121 agtcaatgct gatattatta gaaatagctt gggtttgcta accatgactg tttgtttcat
      181 acctctggct gattgcaaag ctgggtatgc cctataaata atgagagagt aaattggcat
      241 aaatactttc aacattgaat gcacagtctg attggtcact ctaaagtaat aagaaaagat
      301 gactatatta tttttttgca gttcatagaa tctatcgtcc tttccaataa taaatggaat
      361 taagacagta catcgcctat cccacgatca atcacgtctt atagagaaac ttttaacaag
      421 aggaattttc cttgaaagag gagtctacca aaattacatg gtccagtgaa ttaattgagg
      481 tacaagaatg caacgtgtat ttggcacaaa taataccgat cgagcgtatc cagattatac
      541 gttaacgctg tctgacctta aggcacaatg ttttgaaaaa gaaactgatt gagcatgggt
      601 tcgttagaac tggatgctta gagtgtctcg gttctggatt tgcttggcag tatatctatc
      661 aatcaagcgc tttccttctc tgaacaatat ttctaggcca aagtagaagt ccttttttgc
      721 ttttgatacg cttctctaaa tgtcatggta tggtcatatg caattcgaac acacagcaat
      781 cccaacattt agtcaactgt gcctcaaatg tattaagcta ttaatttttg acgatgttta
      841 ttgaaactaa ataatagtag tgtatcagcc ttagcaattg tattgctgct aatatcgtcg
      901 ttagccattg tagctagtga tagtatcctt ttaggtcaag ccaagaagtc atcaacaaca
      961 aaagcaggga acgcaggagt agggagtgga ggagcgggag gaggatttgc agagcaacca
     1021 cccataggaa cggaacctct aactcctgct actgctaata attctgcggc cgccatcgcc
     1081 atcgctcacc gactagcaat aacttctgtt aattcctcca aatctaacgg aggtccatgt
     1141 ctaccgcaac taaatcattt tatccttcca ttaaaagaag ctagatttac tgtaataaaa
     1201 caatgtgtta cggtaacggg tagagtcgta tggacaaatt actttaacaa tgatggcgac
     1261 gctaacttca atgtagtttt agaccctcca tacaaaaata tgttgggacc tgggagctac
     1321 tctcaggtgt ttgccagtaa ataccccgga ggccctgcct tgcatatgga aactgtttgc
     1381 caaggtccag ttacaagtaa gagtaaagaa aatgtaggag cgtgttacgc atataatgga
     1441 ccagatttcc gatctgtgtt gccgaagata ggacagcatg tgatggttac cggaagatat
     1501 ctaattgagg tgcctgagtt gccgggagga attacagagg tgcatccagt ttatgcaata
     1561 aagattcttc cttaattttt ttagaaggac atctccatgc aaacaattat tctagacaat
     1621 tcataaaaga gccggttgaa cctacattag ttgtgtgttg ttgtgattca atttatcttt
     1681 atttgtagtt gttgcttata tccatttcct tatctatata aacacatgaa acttctatat
     1741 ttcatatttg atatgtaaat gcagcgtgtg tgtgataata aatggtaagg attagtgttc
     1801 aagcagctca tgagcaggtg aatccaattg atctattaga tgatgtaata aacatggatc
     1861 aaaatgggat tgaacgatgt tggacaagcg atcattatat gccatggtgg aacagcggag
     1921 catctggagg cgctgcatgg ccgtggctag gagctgcttt agccaagaca acgaaaatag
     1981 tcataggtac tggagtaaca gctcccatac tacgatatca tcctgcaatt gttgctcaag
     2041 tctttgctac gcttggatat atgtttccga atagagtatt tctcggaatt ggaagaggag
     2101 aagctcttaa tgaagtaaca tctggtcatc gatggccttc caatattgaa aaatttcgaa
     2161 gactaaaaga ggcaatacat ctaatcaaga aactttggat tgaagactgg gttgacttta
     2221 gaggagatta ttattgggta aaggattcta ggctctatac aaaacccata aggcccattc
     2281 ctctttatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg aagagaacgg ccagatcatt
     2581 ggcgacgaca cattggagaa aatgttagtt gtaatatcaa atgcagagga gggaataaag
     2641 aaacttcaga agtatataga gctggggttc acagaaattg ttttaactaa ttcaagtcct
     2701 gagagacacc gattggttaa gttaattgca gaaaaaatag ctcccaattt taaataattt
     2761 acgactacag ttaacaacac aatgagaatt cccttttgag accaacgaag ttttctttat
     2821 caaaacagac ggaaataatt aggagtgatt cggaatagga aatctattct cccagatttt
     2881 tggaacagtt ctggaacatc ttccctggct ctagttatga cctttgctag atctctctag
     2941 acattttgcg gccagttctt ctacgccaat ctttttccct taaatccgta ttttgtgaat
     3001 ctgataaggg ttgtggctta ggctcagatc tgggttcttc tcccatttga ccgctagtag
     3061 cgtcatttaa tgcttgatct cgctttagtg gattcttttt ttccacactc atatagtctt
     3121 ttagtggtga atattgttaa taacaatttg ttcataatta tatagctaag aagtacttta
     3181 cactgtatta tgttattgag aataatgtgt tcttgaagcc ctttacctct agcaataagg
     3241 acttaattct aagacagata gtattccttg ctctacaaca aacaattcgt ttcggtgaat
     3301 atgtacctct ttgcagcagt tggcttaatc atcgccgact gattgcagaa tgcaacgcag
     3361 tgtaatgtcc aatttataca gtaaagtact ttatgcggaa accatgggtc gcttgaagct
     3421 aactaataca acctcagcca cttatctact atcctttgac tttagcataa ttgctctatt
     3481 aatgacttaa caacatcttc cagagacctt tcttttccat cttttgcaat atgttcagct
     3541 cctatcgtca tgattgcctt cttagtgtct tcactaatcc gaagaaatca attctatatc
     3601 taatacatat tcaaccaaag gttcgcaaaa ctatggttaa aattggaaac attgcataaa
     3661 ttacaagaca atgctcgaac gataccctca taaccttcct tggattgctt gagtgaacca
     3721 atctggcaag atgtttctat tcttcctgac aaagtatcca aaggcggcgt ccaaaacata
     3781 tgtctttgcc caatcttcct ttgaccttac ggaccttcca tagccttgta tcaatttcaa
     3841 tgctgtttgc caatagtacc attcttcatc catctttctt ttcgcatctg tccatctgtc
     3901 actcttgtta ggataaggca ctttggttat aatctgaaac ctagacaggt catcctttag
     3961 gtcaagaccc atatgtaatg atggtgaaat tagaaccgtt ggttttacac tatgtgtatg
     4021 ttctgcaata acatcatctc tttgtatctc tggatctgta actaatagtc ttcgtacatt
     4081 tgcttgtgaa atattttctt tgataaaatt tagctgatcg taagatgtag tatgaattat
     4141 tcctttatga tttctgtgta aggtcatcag gttgtcgata gctctagaaa tattggtttt
     4201 aacctcttgt agttggaggt tattaaaatt tagataggca atactcattg gatatatagg
     4261 tctattttgt agtggaaagt ctgagtcaac ctgtatgaat ttcacatcat tagaggtaag
     4321 gccaacgttt cggcaaaatt ctttgctgtc aaggattgtg gcgctcatta tcaaagtctt
     4381 ggtgcatttc tcaaatatgt ccttacaata aggagataca tcaagtggtt ttagttcgat
     4441 ccttacgatt tcataatttt ccttccttat ttcagaaata atccagtttt tagtatttga
     4501 aagaatattg tttattgcac gtgtgagtct ttctgtgtct cttattgctt caacagcaag
     4561 ttcgtcacca atagatgagg atctttttga gagattttca ccgtactttt ctgctatttc
     4621 ttcgtcgctt tcaaatatct ctgaagcacc cactaccctt tttttatttt tagatcttga
     4681 tttagaagtc gcattgttta tccagttata tctcactctt ctggatattg acaatgattc
     4741 caccatcgag ctattgccaa ttaaatcaag catttgtgtc tcaagttcaa tgaggaaatc
     4801 aatccacttt tctatatcat cataaccata gtcgatcatc tttaaattgg gaatatatcg
     4861 tttccatctt ttttttgaaa tagatactcc tctatgcttt acgatctcag tttcgagtag
     4921 gtgtccttca tccaacacca acagtttcct ctgtggaaga actttcttat ttggtagaag
     4981 ggctagaaaa ttggaataat tgaatataga atgacttgaa gttaaggctg cattcaattg
     5041 atcaaagtac tcgcatggca tccattctct tcgctggtct tttaaatttt cgaggtgcaa
     5101 ccactgggaa tattcttttt ggtaataatt cttggtatca aaatcaatga atacattttc
     5161 atttgtggtc cccttattac ttactttata gttttttaga aatgttctat acttacaacc
     5221 actatctttg aaggattcat tactcataca tgggccatgt tctactgttg tatggtagca
     5281 ttcatttaca ttatccgaaa cgcatgctcc acatctataa gtatcgttct taataaaatc
     5341 ttctttcact agacatggaa aattgttcat tccttttgct actttaaaat atggaaaatc
     5401 tcttgagtat tgagtctgta agtctttagt tgacgtacat atgtagcttg accccaaggt
     5461 tagtgcaaca gctacggcta cagggctctt tccaaagcca gtaggggctt ctaatagaat
     5521 gtatctataa tctgaagcaa aagccgtaca tatttcattt aacacgtaag cttgtctatc
     5581 acgtttatcc gaaaatggaa agtttttcaa aaagtcagct attgttgttc cttgttggtt
     5641 atcggtcaat tgtgattata tttccttact attgctatga tgtcatatgc ttagaaaaga
     5701 aattatatac ttttgcacgt tcagtggatc agatttgcca tagcttcagg acagaattta
     5761 agaaccgtta aaacttcata gcacacttgc tatttcgatg cttcaagcat atttttgctt
     5821 ctttcacctt cgaaatcgtt tagccgacga atgcattatg cctcatgcca ataacgtcat
     5881 aacatccttt ctggtatgct ggattttgcg gcgaacatac ctaaaattta agagatttac
     5941 ttccactacg tttctcgtgt ggtgaaccat tttggtgacg aataaataga aattttcaga
     6001 gcttcatcag cgtagttatt gtcattacaa gcgggttatt gataatcatc tagtcataag
     6061 aattaaattt gaatctgcta gttacaaagt gttcaactac acccaaattg gcgaaacaac
     6121 attctaaaaa ctaaaagtcg taacgtaacg taagaacaaa aaataagata gttaggataa
     6181 ccaaatgcat agaattacaa tcaggaatat ccataaaaac gacataccta agataattgc
     6241 tttacaaagg aaatcatttt ccgatatggc tacttatgga atgatatggc catcagcttt
     6301 tcttgagaat cacatccgag ttttccgtat cggtcagcta tgtgctgagc tcaataaaga
     6361 aaaaacagtt gttgcatctg cgagcagttt aataatttcc ttaaaacccg catatagaga
     6421 acacacatgg catgaggtta caggacatgg aacgtttaac aatcatgacc atctgggaga
     6481 tagtctttat ggagctgata tctccacaca tccaaagttg caaggtcaag gtatcggaac
     6541 aatgctgtat ggcgcaagaa aagatcttgt cataaaatta aacctaaaaa gaatgatcct
     6601 cggaggtaga ttatataact attacaaata tgctgacaca atgacgcctc tacaatacgt
     6661 ccaaaatata attaatggta aattctcaga tcctgtcctt tcatttcagc taaaaaatgg
     6721 atttagattt attaaagttc ttcccgacta cctatatgac aaacgttctt taaattatgc
     6781 gacattcctt gaatggataa atctgaaata tcgataagag ttatcataca tcaattaggt
     6841 aaattgatgc tagacttgtg aaacggacaa ataatagatt atgagagcat taacattttt
     6901 taggctttaa cactcggatt ttctacatat cttaaagtac aatctagttt tataatatca
     6961 tgtttatgat atcatttttt gtttaacatt attttgttta catattgggc taatagagcg
     7021 ggtacccacg aacctattta cattataaaa aagcattttg gtattttcgt actatcattg
     7081 ctagtactat ttctatcacg ctaatattat ttttattaag tttgttgatt atgataaatg
     7141 gcaactcaac aatgatagca tcatcaaatc tacaaatgat ataaaaatca ttacttggtg
     7201 aatacgctat acgaattaga agtatttcat aagaaggttc atcaagaaga gctagtaaga
     7261 aataaattgc cacacaaaaa aggcaaattt tccaagatat tggttgggat agatggttcg
     7321 caaccatcaa tggatgcagc agagtatgcg ctagcgatgg ccaaaaaaga caatgcagag
     7381 ctcattgcca ttcatgtatt atccgcgaaa tcaggctatg agcatacccc tcagccttta
     7441 ttatttggtc ttccagcaac tcctagttcg gtaaatggta ttatacaaac gtctaaagaa
     7501 gaagcacagc ggtggctaga taaagttaag gagcaacagc tagatgatga aaatatagaa
     7561 gtaaaattaa agacagaagt aatcctagcc tcaacttcga tagttgcggc tgaactagtt
     7621 gactatgctc aagaagaaaa cgttgattta attgtagtgg gaaccaaggg aagatcaggt
     7681 tttaaaaggc tcctacttgg aagtacagca tccggagtag taacccacgc gcattgtccc
     7741 gtaatgatag taaagtgaat tatcctggtg catcctcacg gtaagcttgg tgatctatgg
     7801 gaaaaatatc ttcaggctga agcagagttc cattttcagg aattatcagc tgttacatcc
     7861 gagttgatct ctggaagaac aatgtattgc cctaagcatg aagacatgtt tatcaaactg
     7921 ctcagaaaag acatgtcatg gtttatgatg gatatgatct ggtttaacga taaagaagag
     7981 aaaaggattt aacaatacag agacaacgcg agaggcactt cttaagtccc gtcatacgta
     8041 ctcaatatcg cgtgaagcag attcagggat aggattgtaa ctgagatatt tttttctaca
     8101 ttctacaaat ccgaagaata tcatcaggat agctatcaaa aaaaatgtgt cggtagctgc
     8161 tacatagcat cttcagtaga cccattaaga tgtttttagt attcagatca accaattgtc
     8221 attatagggt gttttctccg agctttacaa tattgtcagg atacgatcag aaggcgtctt
     8281 tttctgctaa ccagccaaaa tcatagaatc aaaaagatta aatgtaattt tttacgaaaa
     8341 aaattaaaga actaaaacac taaagctaca gtacacacgg catacttgtc gagcgctaaa
     8401 acctcttgta aaggcaacta atattaaatt attatacatt ccatccgctt tcgcattgct
     8461 ctggcttgag cagtattcgt taataattga taattaacaa ttttgataat taccagtttg
     8521 catttattgg atcaataatt gttagactag atggattggg cttattcgtc tatgccatac
     8581 gttatgccat atctagtgga aggagtagag aaatggaggt agaaaaaagg cataaacttc
     8641 aactatttta tgaaataatt tgtgctattg aagatgatat agatcataac aaagttgcaa
     8701 gaccaactca tattcagcat aattgcagat tatcctatga taaaatgatg aatcacttta
     8761 ttgagttaga acacaaatac atgatatatt gtgtgattct agcggattaa aatcattaac
     8821 tagtaacgcc aagaaattcg taaaacaata taatgatttg ataaatctaa ttgaaagtgc
     8881 ggatttgtaa gtctagttat tatttataca cttttgttgg aactgagaat gacgaaatga
     8941 tagcggcaat aaaagaaaaa tggtaatcat aatctaatta tgaattgctc cctttaagag
     9001 tcccataagg gagtatcgat aaagactacc acacgatcat attatttgat agtcttctgt
     9061 ccgtaatcat tattaaatcc gttgtcagta cgtaaaccat cttgtcaaat acaaatgcaa
     9121 agattttgtt tggcgttgtc gttactttta cgattgtaat gatgttagtc atcggtagct
     9181 cttttccaaa tgtggcccta agtaaatcat cttcacagat atcatctaat accggtggtg
     9241 ccgcaacaag tactagcaac agtggtgtgt caaatttaaa tggattcaat aatcaatcct
     9301 cttccggcgc tcctctttat ctcgcacccc caccaccggg ctcctcttca agggttaata
     9361 atggcggtgg tgtggctagt aacaataagc ttgtaatgat aggctttgat gactcgtaca
     9421 agagccaaat tctatacgcc aagccaatac tagataaata tggttttaag gcttctctct
     9481 ttgaagtttg tacttggata ggaaaaacaa aagacagaca gacttggcaa gacgtagcag
     9541 cattgcaaca tgatggaatg gatatagaat cacatactat gactcatgct catctcccta
     9601 tgctattgtc ctcaccaagc caactgactt atgagatagg gggatcaaaa caatgtcttg
     9661 ctaatcacgg cgttaatgca acaatctttg gatatccgct aaacttggga tctgacatac
     9721 catcaatagt taatgtggtg gctaaatatt ataatctagc aagatctggt agtgcacc
//
LOCUS       DAOR01000191            3703 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42928, whole genome shotgun sequence.
ACCESSION   DAOR01000191 DAOR01000000
VERSION     DAOR01000191.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42928"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1135 a          633 c          738 g         1197 t
ORIGIN      
        1 ttgtcgatat attcaagcac taatatattg attagaaaca ttatagtcac aatctcagca
       61 ttatctatta aagagaatga tacttttagc aatatagata tagaatctac tactgaaaat
      121 cctttctatt caggtaggca gatattaatt tgcgtgtgtt acaacaagtg ttggataagt
      181 gccaaagtag ccatcggatc acacccgctc gatattttag ttctactggt tttttcagct
      241 gtctttttat atgactttac tagtcggcta ttttgtgcag ccagttatag tggttgatgt
      301 tattattgat ttcacggcca ctttcatttt ttacagccca ataatgatat cctcatcgct
      361 cagcaatctg gcagtttaat gaagctttaa aattgcactt aaatattatg ataatcgata
      421 caccattagt gcgaaaaagg aagaccaagc attgcgagtt ctcaaatcgc ttgaagaaat
      481 gggcaagaaa gaatttgtac cttccatagg tcctgtgaag gctaagatca tcgcaaatat
      541 tgttaagaaa cataaaccga aaagaattct ggaaattgga actttgtacg gatattctgc
      601 gatattaatg gctaacctgt tagatgatgc tgcagacagc gtaggatctg ttgtaactat
      661 tgaaatagat aaaaccgtcg ccaagacagc gagaaaaaat atggaagacg caggttttct
      721 gcacaaagta gacataatag ttggtgatgc tttagaagta ataccaaatc ttcgtgggaa
      781 atttgatctg ttgtttatag atgctaccaa agaagaatat cttagctatc ttaaatgtgc
      841 tgagaagtat ctagcgaaag gggctgtagt tatagcagat aacgttggaa tattcaaaaa
      901 tcaaatgtta gattttctag aatatgtaag aaatcaagaa agatacaaaa gccagactat
      961 agaaaccaag ttggaattta gagatgatgt cagcgatgca attgaactga gtattagaat
     1021 cgtttgattg ttgaggatga ttggttaatt atatgataac actgacatcg atgttcgcag
     1081 actaagaaga cgcgaactca ttttcaagga caagaatgta cgaatgtacg tatgtacata
     1141 tgtatatagg ttcttccgat ataataaggg ggaactaagt gttacttcca tggataagag
     1201 actcgtacaa ggtaatggca agcgcgattg atttccccga ttttcgaaca cacatctaga
     1261 ctcctactct attcattaag ctatggcgcc attcttcttt ttataatgta acttaaaaga
     1321 tgaaaatcta tcatgacgta gaggattgct attagtagaa tcgagttata gctgctcggc
     1381 aaaagcacaa agtaagcatg aagaaaatac ttaacacgtg ttgatgaggt cgctgttttc
     1441 tcttgttatt ttccttgtcg atatcactat ttttgttttt cagggtcatt gtctttccta
     1501 gtaggtaaga tcaccaggat tggacctctc tcacgcctat tctgtcgctc tatctacttt
     1561 tcctctttta cgggtcccta gcgcttacat ctcaatcgtc ttaatcagat agtagtgcaa
     1621 cacagaacac gactgaattg tggagttcgg agacaaggag gcaaccttag ttgcacggct
     1681 agcattactt gttggttacg actttttcta ctggtgggat ctggtattgt cccttaacta
     1741 ctgaatcatc tggaatatac atgcgcaaaa gtagggtaaa tggcccatca ggcgcaggaa
     1801 gccaattgga ctcttttgct tgtcctggat ttttagaaga tatagagata tcaagtgaac
     1861 tatcagtatt attctttaac cctaaagtcc tatcacctat attataacga ttaataggat
     1921 tatctacgaa aaagccatct ttatcataca ttgtgagaga ccagaatcct ttaactggtg
     1981 gaacctgtcc tttaccaaaa tgcattaggt agttagtatt attagcagcg ttcaatagag
     2041 ttccattagc atctgtaaat gttagtggat ataccgcttc ctgtggtata ttggcaccaa
     2101 atcctacttt tgcaacggct gcacgcaata ggtaatcttc tccataagtg ccaacctgta
     2161 ggttaacaga ccacccgttt actactgtac ctatatttgc tgtcttttcg tctattatct
     2221 tttcaccttc tgatattcct gttttcagag cttgaatgat agtttgattt gttatgtcat
     2281 tagatggtgt tttacctaga cctattccta ttgtttcgaa tttcttaata agatacgttt
     2341 gatttgcagg tggtggattg ttgactagtg atttacttaa gatatcaaaa aattgaatac
     2401 caagtttcgg tatgttcgat gcaagaggtt caataggttt cgcctctgat gttgttcttg
     2461 ttgtattgtg aaagacagga gtaagcgtaa ggtttttttg aatttgattg acattagtca
     2521 catcttgagg tccattgact aatatcctgc caataagaaa aacgatattg gtaggtgatt
     2581 gaattttact catattcata ctactataat tggcaggcaa tgttcctttc cagttaggtc
     2641 cagtaataag gaaggtaccg cccttttcac cagttgtgcg tgtgccaata taatgaaagg
     2701 aattggaata tgcatccaag aattgcaatg tatagtacct gttaactata ttaggaactt
     2761 taagcacgat tggtccacct ttaaggtcta tccaagaata tgaatacaac gtatctgcat
     2821 taggacgaac aatatctttt tcttttgcag ttgttagttg gccaagatgg ttgaattgat
     2881 tagcgggacc tcggcctggt gccgcgggaa catttggatt tgtgacaaaa gctttcgtta
     2941 cctcagacgt tacgagagga tacccccata tgtaagcaag tgttgcgata tcataaggct
     3001 tattagaaga aagcaattgt ttcataataa ttcctgcatt ggtaatatta gcactatcag
     3061 gtccctcaat gggcgacgtt aatgatgccg ttttattatc gacatctgca tacgcatttc
     3121 gattattgac agaatagccg ttcagaatag ctgaagtcat aataagaaac gaaactggaa
     3181 ttgccatcag catcgatttc ttattatttt taaacatcga tgacaaactg gttttgtttg
     3241 atctcaaatt cattttacct aattctcaag tcttgaatct aggatgcgtc ctattaatag
     3301 aacacctggc ttgggtaaaa acgtgtattc ggtaatattg ccccattgac ttgcgcaccc
     3361 aattcgcatt cattgacaaa ttgtagtgtg ctaccgagtt atttgtctga atgggtatag
     3421 gaaaatgaac ttttttgatc ttgtttgtta acgtggataa gtgactaagt catgaaggca
     3481 gtaatggcag caaataaagt gactaacgat tttaatagca cacagcaatt tcaaggataa
     3541 aaagtgtcga ttatggcgga cagcttatca tttactcaat agaattaacg tagcagtagt
     3601 aacaagtatt attagattat ggaaagcgca taatataaca tgcaattacg caaagcgtgg
     3661 taatcgttat tccgtatggc agaagcgatg tcaaatatgt gaa
//
LOCUS       DAOR01000192           39499 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_6327, whole genome shotgun sequence.
ACCESSION   DAOR01000192 DAOR01000000
VERSION     DAOR01000192.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 39499)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 39499)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39499
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_6327"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    28844..29039
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    36353..36640
                     /estimated_length=288
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11825 a         7886 c         8270 g        11034 t
ORIGIN      
        1 ttttgtcgca ttacctgccg tcatattact tgctgcaaga acggagccaa cagatgcaga
       61 agttgctaac atgacaccca tggctatcgc taaaggcaat cctaagacat attttgtttg
      121 tcgcataagt gcttaggtgt agacccatcc tttaatactt tcaggtaaaa atatgaaaat
      181 agaatacgta ttttttattt taagcttaga cggccttccg ttaaccaatg gaattcttga
      241 gtggaacaac atcaaacctc aaaatgtgat cctgccacac agatgtagta gttattgaaa
      301 ttagtaagga aatctaaacc cctttagatg tataggagat actgcctaat ctagataatg
      361 agccacgata ccggcatggc aaaaagtcga taaaaaatat tcgcaaaaac ggaaacagta
      421 tcgaaagata agttgggata gcatttaaaa agttaaaatg tatagagatt gtgaccgaag
      481 attataaaga atccattcaa ataaaaatta cgagtggact tgcccagaga acaaaaacta
      541 tcatgatcca tccaaagatg gatgtgcttt ggaatgttag actcgccggg atagtgggga
      601 tttttttaca ggaatagtca aaatgcaaat tgcacaagga actgaggtaa gatatactta
      661 ggattgcagc agcagcaaca gcataatcta tatttgtaga ttgagctact agcagcgctg
      721 atattatgga gcttgctatc ttagtcgtgg acataatatt aacggcaatt atgttcgcga
      781 tttttggagt atggatatat ttattgactt atttagtaaa atcgtacgaa cggtctccgc
      841 gactgaaatg tttcgataaa gcgcctatca gtaaattccc caaggtaagc gtaattctac
      901 ccgcaaggaa tgaagaaatc tacattgcaa ggtgcctaga cagcctgtta agtcaggact
      961 atccaaactt tgaaattatt gcgataaatg actcttcttc tgatgagact ggagacataa
     1021 ttttccgcta ttcaaaaaaa tataacaagc ttgttgtcgc tgtcgaccct ggacctaagc
     1081 ccgacggatg ggtgggtaag agttgggcct gttatcaagg ttatagaaag gcaacgggag
     1141 acctgttcct ttttacagat gctgatacac agcattcacc ctctctcatg tcccttggag
     1201 tagggcatct tttgcagcaa aatcttgatg cgataactgc tatcccaaag ctgcactgca
     1261 tagagacgtg gacgaagatt actctgccga tgctttcaac atttcttcat acaagatttt
     1321 ccgccttgag ggtcaatgat cccaggacta aaattggtta tttttttgga agttttttca
     1381 taatctctag aagtgcctat gagcaagttg gtacgcacga gggtgtaaaa caagagcttg
     1441 tggaagacgg cgcccttggc ggaaaagtca aggacgcaaa gtttaggcta aaaatggttc
     1501 gaggggaaga gaacataacc gcgatctggg ccagggacct gaactcgctc tggcatggat
     1561 tgcggcgact aatgatcccg ctatatagcc agcacaagac caagacatct cttatgataa
     1621 ttgcggtgtt ctttctgttg ttcgaaccat tcattctatt gccatattca tctctgcttt
     1681 tattctttgg cgacagacat cttataatat acattctggg atacgtgaac attctgacga
     1741 tcttacttat agtattgagt aatgttattc attctaagct tagcttgaac caaagcccga
     1801 tctatgcatt ggcatctcca ttggctggtg cgatcatctc ttttgcattt atctcatcga
     1861 tcatagatgc aaaaaagatt ggcgccgtca catggcgtaa cagacaatat acaatcaatg
     1921 aacatcaaca gcatccatta ggctagtggc gttagaattc taattcttta ttaggaatga
     1981 attgaattaa caagcagaag aatatgttgg ctactaagtc tgatcctggc gtatatgaga
     2041 ccttagtgaa gagtttggaa gggagacata tttcaaaacc tgaagcaatg gaactgttcg
     2101 tctgtcaaga tcaggatgcc atcctctcga ctgccaggag catcagggag aagactaagc
     2161 gtggtccaat aacttattca cgaaaaatat ttataaacct gataaacctc tgtcgtgatt
     2221 catgctctta ctgtacttat aaaaaagaac caactgacaa atcggtctct atgatgagac
     2281 cttccgaggt ccttgagctt gcgcaagccg ggaaaaaatt aaggtgcaca gaagcccttt
     2341 ttattactgg agaaaggccg gagcagagat atacccaagc aagatcctgg ttacattctc
     2401 ttggtcattc tacgacaatc gaatacataa gggaaatgag tgagatgata ctacaaaaaa
     2461 caggtttact tccgcacacc aatgctggta gtctgacaaa aaaagagatg tcacttttga
     2521 aacatacaaa tgttagcttg ggcgttatgc ttgaatcttc tagcgaacgg cttgccgcaa
     2581 aggggatgcc acatgaaatg gctccaagca aaaatccgaa agtaagaata aagactctag
     2641 aaaatgctgg cgatctaatg ataccaacta ctacgggatt acttatcggt ataggcgaag
     2701 aaccagaaga catcatcgat tctttgtttt tgattaaaga gatcaaccaa aattatggcc
     2761 acattcaaga agtgataata caaaacttcg cacctaaacc agggacacaa atgtcaggaa
     2821 cccagccacc cgcatacgac tttttcttaa ccgttgtagg agtggccaga atcgtgttgg
     2881 ggggaatgaa tattcaagtt cctccgaacc tgaatccata caatttcgga aaatatctga
     2941 atgcgggcat taacgattgg ggtggcatct caccgttaac tattgatcat gttaatcccg
     3001 aatttccgtg gcccagcata tcttccgtga gagatgcgac gcaaaccatg ggttactctt
     3061 taagagcgcg attacccgtt tacccagaat tcttgctcga caataagtat atctccgaag
     3121 cactagggcc ctacttagag cccctatgcg acggttacgg acttgttccg gaggaatatt
     3181 tagcatgaca ctcgactcta ttttgaagaa cctcgacccc gtaatctcag ggactttgga
     3241 tagggcattg agcgacaagg atatttctgt agagcagggg accgagctgt tcgatgccaa
     3301 cggaacagaa atgaatatga ttctaagggt cgcagacgaa cttcgcagac ggacagtggg
     3361 agagaacgtt acatacgtgg taaacaggaa tattaatttc acaaacgtct gcataaaacg
     3421 ctgtggcttc tgtgcgttta gtcgggattt taggcaggag gaaggttatt tcctgcctac
     3481 agaagagatt ctgagaagag ccaaggaagc ttggaggttg ggcgcaactg agatttgtat
     3541 ccaggccggc ctacctccaa aaatggatgg gaacttgtat attgaaatct gtagagcgat
     3601 aaagaaagaa ctccctgaca ttcacataca tgcattttct cctgaggagg ttctttacgg
     3661 agcacttcgg tctgaagtct cagtcgagga atatttacga atgttgaagg aagccggcgt
     3721 cggtagtctt ccagggacag cagccgaaat attggatcag aagatgcgag accaaatttc
     3781 tcctggtagg atcagcgtca aggactggat aagggttgtc aagcttgcgc attctttgga
     3841 tattccgacc acgtctaccg tcatgtatgg tcatgtggaa tcatcattgc acaaagcaaa
     3901 acatcttgag gtactgaagg aaattcagaa aggtactcac ggctttacgg aatttgtacc
     3961 ccttagcttt gtgcataacg aggcgcccat gtacgataat gataacaata agaataatca
     4021 taataatggc gcgatgtcta acatgaggcc gggccccagc ggcaatgaag tgatgaaaat
     4081 gcatgcagtt tcaagaatca tgctaaatgg ctacataaat aatattcaag tctcatgggt
     4141 caaagaggga gcgagaatgt ctcagctgtt gctcggagca ggtgcgaacg acttcggcgg
     4201 cacccttatg aatgaaagca tctctacggc agcaggtgcg caatatgggc agctaatgaa
     4261 acccaagtat ttacattcga taataagaga agcgggcaga gtaccggcac agcgcacgac
     4321 gctttataca ctaatcaagg tattcggccc tgagccccga gaatctgaat ctagtttgga
     4381 taaggccgat cccagagaat ttggatcgta tcacgaatta atcaagctgg acaaatttcg
     4441 atatagaaac cagacctgat cagtccgaac tcgtaagaat tcgtttacat tattcaggaa
     4501 tttctgacta gacttatcat gcctagtgat gcagccctaa tgtgaccgca tagtgtcgct
     4561 cttagcgcta tagatcgttg catcttcgtt agaaaaatta gacgcgcgct atagtaccct
     4621 taacaaaatt ttcgaattca atgtctgtca ttgacttgcg agtctctatt aatatcgcag
     4681 catcatttcc tactagatag cgaggtaata ttggcttgga ttgtagtgcc ttcagtatag
     4741 tggatgctac ctcgacaggt aatgatccca attcaggcat acgccccatg tcgttagtta
     4801 tcttccgagt catttcagaa tatggtgaag acaatcgttt ggctctttga gccatgacaa
     4861 taaagtttgt aaacgtcgag gaacggatga tcccaggttc gataagtgaa acagctatac
     4921 cgaagggttc taattcatat cttaaggatt cactcaaacc ctgaattgcg aattgtgaac
     4981 tgatgaacgc tgaagaacac ggaaatccaa tttgtcctgc gacaaagtta atgttgatta
     5041 tgaccccgct tctttgtttc cgcatgagcg gtaacactga ccgtaacatt ctgatgatgc
     5101 cataaaagtt tgtttcaaat tgtgctgcca tttctctaat ggaaatatcc tcaaacatcc
     5161 tagtaagatg taactagtac cgttcactaa cagatctatc aacccttcgt ttttcaatac
     5221 ctttttcact gcgctcgtaa cagaagaact gctgttgaca tctagtttga tcagctgcaa
     5281 agataacttt tcaatatcag ctatatgaga tagtttcctg gcttgggcgt gggattttat
     5341 tccggcatat attacatatc cccttcttgc cagggctaac gcagtctcgt agcccacgct
     5401 agtagaagtg tccgatagaa gtgcaacctt cctcaacttc aacttatttt tgatatagac
     5461 gatattaaca cttttgcggg taaggtttaa gaatagtagc gagggtcttt tgtgtgttag
     5521 atccgatgga atcggcagcg accacctcta ccgtactccg ctcaaaatgg catactctcc
     5581 ggcataaggg tgtagcattt cctcctgcct atcaagcaag agggctaagc ataattattg
     5641 gtggaagaag attatcgctc gatccagccc aagaagaatt agtttatgca tgggcaaaga
     5701 aaaaagatac tcattatatc ctagaccggg ttttccagct gaattttttg agtgacctaa
     5761 aaaaattgct gcctaaggaa tttcaaagta ttgataacct tgacgtaatt gacttctccg
     5821 aagccttcca acttgtagat caagaaaaga aaatgcatga ggccgagcta gagagaatcc
     5881 gtaaccttcc ccgggaggag aaacgaagca tgactatcgc taaacgtgca gaaaaagaaa
     5941 aactgaaagc aacttatgcg aaggccatcg tagatgatgt tgaagtggat atagccaact
     6001 ggctggtcga accgccaggg ttgtttatgg gtagaggtca gcaccctctc cgaggaaggt
     6061 ggaaaccacg tgtaggagct caggatgtta ttcttaatct cggagaagac gctcctgtgc
     6121 ccggaggaac atggcaggag atcgtgcacg atcattcgtc aacttggctt gcaacctgga
     6181 cagagaaatt gaccggtaaa agaaagtacg tgtggctaca tgactcggct acgcttagac
     6241 agaataacga gaaagaaaaa tacgacaagg ccaacaaact aggaaagcac atcaataagg
     6301 tgcaaagaga aattatcaac aggatgctca gatctacgga aattaatcaa aagaagatcg
     6361 ctaccgtctc ttacctcata tttaagcttg caatgagggt gggtgatgaa aaagatccgg
     6421 aagaatctga tactgtaggc gcttcaactt tgagagtaga acatatcaaa ttcccagaaa
     6481 gtgatggaca acattttata gagttcaact tcttgggcaa agacagcgtt ccatggcaaa
     6541 aaacactccc ggttgattct caagatacaa gcggccttta cgacaatctc aagttattca
     6601 tgaagggtaa acagccaggc tctcagattt ttgatggaat cacttctagt aaagtcaatg
     6661 cgttccttcg aagcattgat cccaaaagcg tacctggatt aactgctaaa gtttttagga
     6721 cttttattac tactagaaca gtaaaggagg cactagctaa gccaccgata aggatccatg
     6781 agaagtcatc tgaagcacag aaaatctatg tggccaaaat ggcaaatttg acagccgcag
     6841 ttacttgcaa ccacaaaaaa ggcatcgatc caaaaaatcc cgccagtaaa aaagcaatag
     6901 aaagatatga aaattcattg ctgaaaaagc aagagacgat cgaaaagtta aagcgcgata
     6961 tggaagcaag aacatggaaa actgaaattc aaaagagtag actcgagcaa agattagaaa
     7021 agcttgaaat gcaactaaga ctacagaaaa atacccgcga ttacaacctt ggcacatctc
     7081 tgagaaacta catcgatcct cgggtaatga aggcctggat ggggtatgtt gggcttgact
     7141 ggaaaaaaat ctacacgtcc acacttcaac gcaagttcaa gtgggtcgaa gaatatccca
     7201 tgagagagtt aagagcattc tttccaagtg taaaagaaaa gcaagataga gcagtagagg
     7261 tagtccaaga agagagctaa cttgggcggt gtttagttca tttcatatat cgcttggtca
     7321 ttccgcaatt gtttcataaa aattatttca atatgccaaa tatacggaga aaaaaaacgt
     7381 atctagtttg tggaatagtc tttagtttag tattagtcat tatgggctct gaagaaagaa
     7441 atgcgtattc gtatttgcaa accaagtctg ataggtattc tataagttca ctctttgttg
     7501 ccctgttctc agacggcaca gcattaatag aacaagacgt agttctcaat ccgtctatac
     7561 ccaacacagt ggttgttctg tatggcagga gcgttgatgg tctaaatgta atggattata
     7621 aaggaaagtc gatcggatac caatcgggtc agcagccagg tgaggtgatc ataaactcct
     7681 ccggaatatc aaatgccaga ttatcttacg aaacgcctga tcttgttaat aagcagggga
     7741 ggacatggac cttttcgttc aattccacga ctggtttttc cttaaaacta cctacgaatt
     7801 cgacaatcat tgacttgggc aaacaatttc cggcgtctgt cacgcaaatt ggagaacaag
     7861 gattacttac ttttcatccg ggtcatattc aaattagcta cgtaattgga tatttgggta
     7921 cggaaactca gtcgaacgca agtatcgaat ctgcagttat tgcaatccag gatgctagga
     7981 aaagatatcc aggaataata acggcagatg cacaaaacct actgcaacag gcattaacgg
     8041 ctaaagctag caaaaaatat tcagacgcag aaaaattcag caccgcagcg aacgatttgg
     8101 ctatcaatat cacaagagac tacctggcct ctcaaaatgc aatcggaaca ggacagctgg
     8161 aggtgcaaaa agcggctgaa actgggcaca ttactagtgc agctagagca attatttcgc
     8221 aggcagaaaa tgagttctct aaaggtcact acagccgcgt caaagcgcta gtcgatgaag
     8281 ctaccatata tattgggaac aacgcttcta acccatttcc cgtctttgcg ataataggag
     8341 gtatcgcggt agcaagcacg gtttcagttc tagtgcttcg aaaaaaaata atacacaaac
     8401 gcttgcgggt tgaaattgag catgattctg ttggtggtcc attatctgat gattcatctg
     8461 acagacgccc tccacctcag ccttcgtcat ttccaactgc atcgccgcca gcccacgaag
     8521 aacaggatct gcctgtgccc acagcggaac aaactgacct ttctagagca gtgatccgaa
     8581 ttcttgccga aaggcctcat cttcgacctg aggaccagcg tgttctcaag tatttagccg
     8641 agaaggatgg tgcggcgttt gagagtgaag tgcggaataa gtttctgtta ccaaaaacta
     8701 caatatggcg attagtgaag agactcgaaa gggaggaatt aatcgagata aggaaggcag
     8761 ggggacagaa tctgataaag ttgcgattcg agggcgtaga acgctaggag tacgatctat
     8821 caatatgaat tcatcatttc gctcgaagcc aatgcttaat tagtcccctt gatatattgt
     8881 gacacaaaag agcgatgcct acagcctacg tgctaattaa ttgtgatata agccataaaa
     8941 cagaagtcat aaaggagctt agccagctac cagggatttt tgaggttgca gaggtgcatg
     9001 gagcatatga tattttggta aagttaaacc agggaagcgt tgacgagttg aaagaaacta
     9061 taaagtggca tctcaaaaag attcctgata ttaagtctac cgtcacgctg atagcaattg
     9121 tgagtaccga cccaggtatg tcataatctt ggagacgagt gttggaatta ctagctaaga
     9181 gaatccatat tttttccaat ttcggtcaac aactgccttt gtttcatcgt ctaccacagc
     9241 aagttgttga atttctctgg catatccttc ttccttccac ttcgtagtag catcaattcc
     9301 cagttttgag cccaaattta ctaaaggtga tgcaggatcc agcgtatcag tgggtgcatt
     9361 gtcgatcacc atagtatctc gtttaggatc ggatcttgta gtaatagccc atattacttc
     9421 gtctacgcta tgtacattaa tatccggatc gactacaaca aatattttcg taagtgatag
     9481 ctggcccata ccccacagtc ccatcattac tttcttcgct tggccaggat atcgtttctt
     9541 tatggcaatg actgctaagc cctgaaacca acctgcagct ggcatcgcaa agtcaactac
     9601 ctctggctga aatatctgga ttaatggcaa gaacgagcgc tcaataactt tccctatgta
     9661 tgcatcttca agaactggtt tacccaccac agttgtcaag taaattgggt cttttctttt
     9721 cgttatgcaa gtaagtgtga aggtgggaaa gggctctgac ggggtataat agccagtatg
     9781 atcaccgaat gggccttcca tgcgaatatc gtcaggatcc acatatcctt ccaaaacaat
     9841 ttcggcgtta gcaggaacct ccaaatcaac agtgctgcat cttacgagtt taactccttt
     9901 ctttctcata ataccggaaa agaggaattt atctattcct tctggaacag gcgcgaccgc
     9961 ggcaagaacg gttgcaggat cagcgcctat aatgatggca acttcaattg gttttctctg
    10021 ctcttttgtt atttcataat gcagtgcacc gcgcttatgc gtctgccagt gaattagggc
    10081 ttttttgtcg ccgataacct gaattctata aacacctagg tttcgaatac ctgtgttggg
    10141 gtgcctcgta acagttaccc cgaatgtgat gaatttgcca gcatcattcg gaaatgattt
    10201 gagaattggc agacagttta agttagcatc ttgtgtttgc attatttcct taactgggcc
    10261 cgattctgta tgcctcggac cgtagtcgga gatttcggag agtttaggca acattttgag
    10321 tttattcagc attccggacg gtaaacttag cttagtcagc tcaactatac gattaccgat
    10381 ctctgtaaag tcttctgtct ctagagcgat ttgcagtcgt tttatcgtcc caaaggcatt
    10441 accaagaata gggatatcag agccttctac attttcaaaa aatactgcgg gctggtctcc
    10501 cgaatacatc agccggcgca taatttccgc gatctccaaa ttaggattta ccttcgtctt
    10561 tatccgcttt aattcgccag ctttatccag cgcttcaaca tactccccta agctttcgaa
    10621 cggcatgaga agtatatcaa ggagaaagat tagataagta ttctctagtt ggaaatagga
    10681 tgatgttccc aacggcgtcg aatcgacggt acagcagaac taatctgttg tgcctagaaa
    10741 tgacatcagc cgatacctcc agctcagctc gatttatgaa tggttttgca ggtggtttac
    10801 tctcgaaggt agatagaaag ctttttcata aaaatgcggg attcttctag atatgttaaa
    10861 ggaattaact caaaatggcg gtctttttca actgagcctt taattatggc actcttgctc
    10921 tcgcagcatc aactaatcga atagttgtag ggactcgttt caaaacgcct cccttctacg
    10981 aggatcagta agaagacgat ttcttcatag atagctagta gtagatacaa tcctagtatg
    11041 aagggtacaa ctactagccc ctatgaaaca aagtcgcaat ttctctaccc aaatcattat
    11101 tacaaaaagc aataaagccg taaagatata cgaagaagca tattcatccg tagatgagtt
    11161 gagtagaaca atatctccaa tgttggacga caaattactc ttggtacgca taccggaagc
    11221 ttgattggtc gccaaaaaga ttgcaaaaaa aggtataata attggtctgg ttgttgtggc
    11281 accccgcgaa tctagtatgt taacaactaa ttcatgcaag ttggaataac cttgacttac
    11341 tgttccaatc gacgcaaata gtattaggat actaatgaat accattcagc agttctcaaa
    11401 tagtaacagt ttgtgtcaga agtggatcat aggtgaatat tataatgaca gcatacctca
    11461 agataacaca ggcttctggt atgtcaaatt acccggtggt gattcctgaa ggccaggttt
    11521 acagaagata acagtatgga tccccaactt gagcctagta ttcggcaagc ctatttttgt
    11581 tgcatttgtc tgcagcgata gaaatgtggt catgcattct catttctgct cccattagaa
    11641 atccttgttg aacgtaaaca gactagcaca accttggccc ccgttcgcga gcgatcctgt
    11701 ggccttttca actgcattct atagacagtt agatgagaca ctcaactata actatctatt
    11761 atatcccgcc accaaccacg aacacagagc taaggtgaat atgcatctaa catacatgat
    11821 cctagccatt tgccaaaggt aataggcata cacgtacatg atgggccgaa agggagttga
    11881 accctcgacc tttcgattat cagtcgaacg ctccaccaag ctgagctatc ggcccctttg
    11941 tcgcctaaaa tctctcaaaa taagtgttat ttaatcttgt ggctcaggac agtactgtga
    12001 tctctaaatg catttaagag catcgtgcta cctagatcgc atggtaaatg gctgaatcaa
    12061 tcagcaatat agccataata acgaaagcga acagcattga agccgaggaa gccgctagaa
    12121 gaattacaag attggttgca catcgagagc ttactatata tgctgtgaat ccgctgacga
    12181 tcgaagctag tcgaccggtc gaagcagaag atctcggaaa acttgaactt gacattgttt
    12241 ttgcaatagg cggggatggc acaacattga gagcatttag atatatcccg cgggagacac
    12301 ctttattgag tgttaatgtt ggtggcaaca gaggcatctt atctgaaaca ggaattgcat
    12361 ctttggacga atctataaat gctctacttc taggccagta ctttcatgaa cgccgtataa
    12421 gaattcaggc gtctgtcggg cagaagatca ttccggcagc tttaaatgat atcatggtaa
    12481 caagggtaaa tctgacaaga actcctctgc tgtccataaa actgatgggc gatgtgatag
    12541 agcagaggat ggatgggatc ataatctcta ctccgaccgg atcaacaggg cactcgttct
    12601 caatcggtgg tccagtttta tatgagaggt tggaatcatt gctcctttcc ccgatcgcgc
    12661 cgatgaataa aatgccacgt ttggtgcttc cacccgacga aatcgaagtt aaaagcacgc
    12721 acgatctcag cttagtagta gacggacaag aaatattccc gatcgccgca ggcgaacctc
    12781 tgaaaatttc tcgccatcag tatgacgcac gattcattcg aattagaaaa agaggtatga
    12841 gacagattgt caagctcgga ttttagcgaa ttcgttttgg acgcaagtgc attttatgct
    12901 gggattccgt tccagtcttc aaagaaatgc tttactacca acgcagttta taatgaagtt
    12961 aagcacataa gaagatctta ctcaccgtta gaggcactaa ttgacgcagg taatttgata
    13021 gttatagagc ccgaaaaaac ctcgttggat aaagtcatag ctacagcaaa agaaacaggt
    13081 gatagtcgca aactatcatc ggcagatatt tcaatacttg ctcttgctct tcaactgagg
    13141 aagactctaa tttcggacga ctatgctgtg ggaaatgtcg caacgttcct gaaaatccca
    13201 gtcaaaacac tcgccttcaa gggcattagt gagttgagaa cgtgggtctc gttttgtaag
    13261 gcgtgcggtc agacgtacga atcttacatt agcgtgtgta ggatatgtgg atctcgactc
    13321 agacaacgat tcagggtggt aagtgacaaa gcctaacagg gatatttcag cacctgcttg
    13381 ttgcgtttct agtttcaatt atcgaggcta atcggaatct gtctcttcgt tcgatttagt
    13441 tgagattggt tttgaaagct taatgtgaat agtatcctca acgccattga gtttgaagtc
    13501 tcgtattatc tttgggtcat tgccgacatg tttaaggtag gtcgcaagaa gcaataccca
    13561 acgtgatacg gattcgcttc caacctttga tcggatactc attaaattat taattttgtc
    13621 gtgagttaaa ccgccggaac agctaatacg taaggcgcag tcaatgatgg taagcatttc
    13681 atcgataccc gtatcaaata tgtctattcc taatttctgc gcagacttga ttaaagcgga
    13741 cgtaggttca tcccaatttt tagagatctg tccaaagata tctgcacttt cgaggatctt
    13801 gactaacttg tttgctttca taacgatgtt tggtcgcata tttacaattt gcctgatttc
    13861 gagcgcattg ttccactctg tctcaaaact tcgcaattgt gacaaaccga atatgtcaat
    13921 agatgcaaaa agagcagctt ttccgttact gctatcaact gcaaccatat tattagagtc
    13981 gataattatc atattagaag tcacatttcc aattttcaga tcaatgtctt ccggaagtga
    14041 tggcaggttc tcgttcccga tacattgatt cgctaataac agccgaatgt tgacgccatt
    14101 agtgatagct ttgatcatgg ctaatttaca ctgtattatc agttgtattc cccagctatc
    14161 cagaatagca taaacagagg acttcgaatt ggcaattagg ctcttcattt tctctaatgt
    14221 ggaattggga tctaggatgt aatacctcct ttcctcggaa gccttaggtc tttgctcttc
    14281 agtgattctc tgaagtctat ccactatctt tttcatattc tttgacctgc gttcgtagag
    14341 ctttactatt tctctaaacg cctcttcagg aggaatggca ctgcaaatta agggcttgga
    14401 ttgagaaact attgataatc tttttttttc caatttcttc agagtagagt acacttttgt
    14461 acgaggtagg tttgcgtagt atgatatttc actggctgca agcgagcctt tacgaatcaa
    14521 tgtaagatac gctcctgctt cataccttga taagcctaat tcttctaagc taccaactag
    14581 gtcgttagcg ttatgtcctc tgtgcataga aactattcag aaaattgtga cttcgactga
    14641 aggtaaagat catagttaaa ttcattgcat caaagttaaa aaaaatactg agctgttacc
    14701 ttgtgctata gtaaccgtag ttacgaattt attcattaca tggatggtat taatgatggt
    14761 atccggtttg acatagatac acgaatggaa tccagatcca gatgggtaac atcggaattt
    14821 aggtcaggag acgatggcaa gtacaattca atgaaatatg acgtgtcttt acaaccaaga
    14881 attgatgcaa ttctaaacca tattaccgag cttgaacgaa taatgtgtca atcaatatca
    14941 aaagatggcg acatcagtcg gaataaggat ctgcatcaag tcaaaaatca cactattcat
    15001 tattctgata tgtccgcctc ggaatacttg tcgacacgaa aactgaacca ggtcattcaa
    15061 gtgtcaaaaa tattactcga gaggttggca tatcaattga ggaacgtttc aaatctcagc
    15121 gatatagtaa tcatattaag ccctgctgtt ggaatactca agaatattcg ctcttcattg
    15181 attccatgca tacctgattc tcgtcaagaa ttgtgttata tttgtgaact gttggaagtt
    15241 gtcctggtag atgctgccca attgggtgga tacacaataa actttcaaac agcaaatgaa
    15301 agtgctttac acttgataag tgaggcatcc ttgaataaag ggggagaact aacaaaagaa
    15361 ctttcagagt tacgcggcgc actacgaaat gactaaattc ctagagcact gcgcacatgt
    15421 tgctctgtgc acctccattt accactcttt gagctgaaat caactctacc cctgacgtag
    15481 tatcttgtgc aaattatttt gtttcttacg caacatacat ttacatcata gtaattcaaa
    15541 cgttgtgtta attattattt tattagcggt aatgaatcat tatgagtctg gctccccaag
    15601 aattagagaa tagcgcaagc aaatatgccg cagaagcaat tcgattagat tcacaaggct
    15661 cacgaggaat ggccatacag tcatatcagc gggcaataga atctcttgta aaactagtac
    15721 aggtctatcc agaatacaaa ttaaataaaa tctatgtgga gagagctggc gcatatcaaa
    15781 gtagaattga agcgttacga atgtcacaca agctcgatga aggcagcttg acgaagccag
    15841 acgaacccac taaggggaac cggacaatta attcaaatca gtacgccaag gctgctggac
    15901 gcacaaatgc acaggccgct gaaccgtcgg atggtgctct agatgatctc gtaatgaagg
    15961 aaaagcccac agttcactgg cacgaggtta tcgggcagga ggatgcgaaa cgagccatta
    16021 gggaatctat tgtctatccc acaaaaagag cggatttgtt ccctctagga tggcccagag
    16081 gcatactgct ttacggacct ccagggtgtg ggaagacgtt aatcgcagct gcgtctgcag
    16141 cggagattga tggctacttt atcaatgttg acgcagcttc gatgatgagc aagtggctgg
    16201 gcgaggcaga aaaaaacgtc tcgaaattgt ttgtgatggc acgtaagctc aacgaccgtg
    16261 aaaatgttcc ggtactcctt tttgtagatg aaatagactc tctactcgga accagaaacg
    16321 gcgaagtcgg tggcgaagtt aggctaaaga atcagttcct taccgaaatg gacggaataa
    16381 atagcaaatc aaaagagcta ctcctctacg tcataggtgc aactaacaaa ccatggagtt
    16441 tagaagcagg attccttagg cgattccaaa aaaggatcta cgtaacgctc ccagaccata
    16501 gttcgagaac caatttattc gttcaatata ccgcggcatt gaaaaaggaa ccctcgttga
    16561 gatccgaaga tcttgccaag ttaggggaag gttatagtgc aagtgacatc aaggacattt
    16621 gtcagtcagc gcagctcagg gtagttaacg agctatttga aagtggaaaa gctatggagg
    16681 caaagtcaaa tccaagagcc attaacatga ctgatttcaa agaaatgttt aagatcagaa
    16741 aaccaagtgt acctacggac atgattagag catacatgag atggagtgac cagtttaagg
    16801 cactctaaga gtcaaaatga aatatgccac tcttaaagcg ttagactacc agatctgggc
    16861 ttttgaattt gttcattgct tcggaaatct gcttctttgc aatatctctg cctttccagc
    16921 ctataacctt aacatatttt ccctgttcta actctttgta gtgttcaaag aaatgggtaa
    16981 tctgattacg caaatactca gggatgtcgt cgacttcatt aatggttgaa aagcttgggt
    17041 ctatcttgtt taacggagcc gctacgattt ttgaatcatt acctttctca tcctccgtca
    17101 agagtacccc gactgggcga gctcttataa cagccatagg tattatagga ttgttaccga
    17161 gtacgagtac gtcaactggg tctccatctt cttccttcgt cttaggaatg gaaccatagt
    17221 tacatggata gtacattgca ctggccaaga ttctgtccac gaagattaat ccgttcgtgg
    17281 tgtctaattc atactttatg ctgctgcctt ttgggatctc tattattaca ttgatttcct
    17341 caggagcgtt ttcgccagca ctcacggtat ctttatgcgt caaaatcagt atttaccgct
    17401 cttcagagag aatatattct tattgcattt taaacaaaaa acataaacac gagtcaagag
    17461 gagattgcca ctcgtgcaga tggaactagc aggaaaatac gatgcaaagg ctatcgaaac
    17521 tgagattcgc cattatctcc acaatatcga cttgagctct cttttggaaa acgaattgcg
    17581 ggataataaa cagctggttg cttttattga gggtccccca acaatgaacg gcgaaccgca
    17641 cgccgggcac ctaagggggc gcataatcaa ggatctgtgg taccggttta gatcacttca
    17701 gaagaagaag gttctgttca gagcaggctg ggacacgcag ggtttacccg tcgaattgca
    17761 ggctgaaaag gaattaggct tgaccggaag taagatggaa aatgtaaaca aagtaggact
    17821 agaaaaaatt gtagaggcct gtaagaaggt gattcagagg tacaatgaaa gttggattac
    17881 cgctgacaag ttattgggca tgtctttcga ctatgaaaaa gcttactgga cgttccgcga
    17941 tgagtacatt gagagagaat ggagatatct taaaaaagca tgggaaaccg gaatattgac
    18001 agaatggttt agggtagtgg catattgtcc aggatgtcag acgtccctca gcaatgctga
    18061 agtgaatcaa ggatacgaga atgttgaaga tccttcgttg tattataaag tcaaattatc
    18121 cgatcaggat gtctatctag ttgtatggac tactatgcca tttacacttg taacggatga
    18181 aatggttggc gttaatccaa atgcagcgta tgtttacgtc aacgtaaatg gcgaggtgtg
    18241 gataatagct gaaaacaggc tgcaagattt aatgagcgaa ttacgggtga cagactatca
    18301 cgtggaaaga actgttttag gcaaacagct taacggaagt cattatgttc atccgctatt
    18361 aggactgatc ccaaagttag aggaactcgc tgcggcagga tctatacact ttatagtagc
    18421 ggaggaattt gtggatattt ctactggaag tgggatagta catctgtctc cagctaacgg
    18481 cgagaaagat tttgaaattg ctgcaaaaag gaatctgcca atttttgtac ctatagacga
    18541 cagggtaaat tttacctcag aagctggatc cttcgcaaat actttcgtaa gggacgcaga
    18601 tttacgagtc gtggaagcat tgaagacatc gcatgctcta ctcaagatcg gaaagatcaa
    18661 gcaccaatat cccacatgtt ggcgttccca acataaacta gtctggcttg caagacggga
    18721 gtacttctat atcatagata agttaggaga gatgccgacc gaagctgcaa atggagttga
    18781 ctattttttt gaagctccaa aaaatagatt tctggaaatt attaaagaga aagttccgtg
    18841 gtgcatcaca agggagcgta tatgggggac gccactgcct atatggacat gcaagaaatg
    18901 tcaacacaaa gatgccatgt tttcgagaga ggaaatcttg aagaaatcga tctctttgcc
    18961 agatggtcct ggcttcgagc tacatcgacc gcagatcgat aggatcgaaa taaaatgtga
    19021 aaaatgtggt tctaacatgc gaagggaact cttcgtcttg gatacttggc ataacagcgg
    19081 ggcagctccc tacgcttcac ttagcgatta cgagtacaaa aatctgattc ctgctgtatt
    19141 tatgacagag ggaatagatc aaacacgagg gtgggcttat actctattaa tcgagaacgt
    19201 tcttttgaat caaggcgcag tccccccatt ccgatcattt cttttccaag gacacgtact
    19261 tgatgaaaga ggtaacaaaa tgagtaaaag catcggtaac gttatagatg cccacgatct
    19321 acttggggaa aatcctgttg atttaattcg attttatttt atttggaagt cctctcctat
    19381 tgattctctg aagttcagcc tacaggaaat gcgagcaaga ccttaccaaa ttataagcac
    19441 attgtattac ttacatgtat actttagaca gaacagcaca tttgatggat ttgatccacg
    19501 attacacgat ctaaagtggg tgctagctaa tgacctaatg tccatatctg aaatctggct
    19561 catatcaaaa ctccaaaacc ttgtgaagca agtttctcta gcattcgaaa gatgtagatt
    19621 caacgagggt gcaaaggccg tggaggaatt tataatcaat agtgtaagcc aaatgtacat
    19681 ccccgttact agaaatagta tctgggatga cgatccagaa ggcctagatc gaagattggc
    19741 catttatgca gtgttggcac actctctaga acgaatagac ataatgctga acccgttaac
    19801 accattcgtt acagaatacc tttacatatc atgtttccca aataaaaaga gtgttttgct
    19861 cgaacgttgg cccgaatatg acgataatct tgttaataat atcgtcgagc aggctataga
    19921 taaatgcaag gaaattatat cgctctcaaa tgctgctcga atgaaagcca ctttaaagag
    19981 gcgttggcct ataaaggaag cccttatttg tacatcggat actaacttgt tacaagcaag
    20041 aggaattgct gaaatattga taactcagct gaatgttgaa agctaccgca tttttgaggt
    20101 tacaaatgca acacttctac aaaaagtgcg aagtctaatc gagaatgaat tacccattgt
    20161 tcccaatgtt tttttggtaa aaaagaatgt agcatccaga gtgaggtccg atatcgataa
    20221 ggtcagccga gctcttgaca gagttgataa attggaactg ataaagtctc tggagacatc
    20281 gggcagatat tcgctgtcct atgacagtag acaaatagac ctttcatcca acgatttaga
    20341 gttctcatat gccataatgg aaggttacac aatgtcagaa cgagacaacc ttatggtgtt
    20401 catcgggaca aggcgagata aagatttgat tataaaggga atgctaagag atcttgcaag
    20461 aaatttgcag cagcttcgaa aggagcgtgg gtacaacccc acagagatag tttcaactgc
    20521 ttttgttgca aatttatttg aagaggaaat atcctcttta tattcaatga aggaagaatt
    20581 gttacatcta gtgcgagtca aatcagttgt tctctccaaa gcatctattg agaagattaa
    20641 ttacaaaaca atccagcttg atgagagggt attagaaatc tccgtagaat agaagaacct
    20701 tttcactaaa aggtgtgcaa tattattgct ataagctaat atatgcttgg gccgactacg
    20761 actagttaat agatgtatca aactttaatc ggccgcgcag tagatggcaa gatacccatt
    20821 atgtcaataa atattctcat atgtttataa ataaaaacgt gacatggttc taaccaagtt
    20881 gacaaataaa caagtaacca tcgacgaaaa gcaagtaacg ctcgacgatt gggtaattga
    20941 gaggttcaga gagaagctca agaaggcttc tgtcattgca aacagattgg gaaagcctat
    21001 agtcttgtac agaaagacgc tcgaagagtc cgagtctgct ggtgaagaag aagtagccac
    21061 acttgtcaac gaaaggtatg ctctcgttca agtttataca cacggaggtt ttatccccct
    21121 gagctttcaa caacaacatg tattctccct aaatgaattt cctacgtgga tgatgaaaag
    21181 aagtcgagag ctagttctgc agtgtattga ggcaatggat gattaaccat caacagatta
    21241 gataaattaa gaagtaatgt ccaaattctt ccgacattaa tggcgttggt atgaatagta
    21301 atatctgtgt ctaggcattt tagcgtacat tacacacctg ataaacagtc agtttttgat
    21361 ttagagtcgt attagacgga taaagttatc gtcctcgagc aaaattaaca aagttgttga
    21421 caagtcatga taagtggcgc tctgtatttt gataatccta caaatggtag atgtctcctt
    21481 gtatgttcca tttattcaaa gatggtcgcc agggatagag gatgaataaa acggctgagc
    21541 gtgtaaaaat tactcaataa atagtggaca tatggaagac aaattaatcc taccaagcct
    21601 agatccgaat ttagtactta gtattagaat tttcttcggt cgtgatggca tagtacattc
    21661 atatcatgag atgaactaga tgtatcctta atatattaat attataatta ttagttatac
    21721 cagtaaatca caatgattaa taattttatt gcatactagt acccacttga cagatgttga
    21781 gttagaacgg ataatgaagt cattagtcga ggagcaattg attgtcaagc tacttagatg
    21841 ctttgattgc atcaatgagc acgacaatgg atatcgtaac aatgaagcta catcaccgac
    21901 aaaccctcgt ccactgcgat tcttaaattg aatgcccaaa taatgctatt ttgaaaatag
    21961 tggtagaatt tcttcgagac tcttattaag aaatcgtgtt tgtataatgg tatattcagc
    22021 ctttagcatc tgttcttaga aggtattcgc attgaatatt tattagaaga atttgttgat
    22081 aaggactgtc tgcggccatg aggaataaaa gtaaagtgac atacgccgac ttgggaatag
    22141 atataaaaat gatatccaaa gcccaaaaaa aaattggtgc tatgatttct gctactcact
    22201 tactcccatt taccggtaaa gttctttcgg gctacggcgg ttatgcagga ctgatcgatc
    22261 ttggtagtag aatattggcg ctacatgctg atggcgtcgg atcgaaaata atagtagctc
    22321 agatgatgaa tcgtttcgac acaataggca ttgattgtat tgcaatgaac gttaacgatg
    22381 tcatctgcgt cggggcacaa cctctagcat ttattgatta tatagcgcta aaatctgcaa
    22441 ataaaattct gttgcaacag atcgtaaaag gtctcgttgt gggagcagaa gaatctaacg
    22501 tccctattgt aggaggagag acagcaattc tcccggatct tatatcgggg ttcgatgatg
    22561 ggaacgcttt tgatcttgca ggaatggttc tgggaagcgt agcgaggaag tcggagcttg
    22621 ttttaggtca aagaatcact gtaggcgatg taatcctagg agtcgaaagt agcggattac
    22681 attcaaatgg ctatacgttg gctcgaaaag ttcttctttc aaaatattct ttagatgaaa
    22741 tccctggata tatgcatcat atgctcggag aagaactact aacccctacc cgaatatatg
    22801 tcaaaccaat aatcgaaata ttaaaccgca gaaagagtat tcggattcac ggtctagccc
    22861 acattacggg aggctcattc acgaaattgg cccggttaaa taaacgagta aggttcatgc
    22921 ttaataattt gccttcagtg gagggcatat ttaagcagat acaagttgag ggacgcgttg
    22981 acataaatga gatgtataga acttttaaca tgggaatcgg attttgtgta atacttccaa
    23041 ggaactccgt cgacaaagtt tttagtatat ttgagaaata cagaatgaga tgcttacaga
    23101 ttggaacagt tgataaaaaa ggcagcggga atgtaatagt cagattaaat ggcaaaaata
    23161 atgtcctgta atccccagtc agttgctgat gctaaaggta gacctccctc ctccggttga
    23221 gttcggaatt attgttaccg atacgtttcc ggataatatt aatatgtatt ctgccaatca
    23281 gagtcagctt gttgaggaac ctctcgctac ccagcgaaac agaagaaaaa cttgaaaatg
    23341 actatgtttc cgcgtgcaag catatcgctt cacaaattca attgaggagc gacatatcac
    23401 gccgtgatat tgcgaaaatc gtgagagacg cgtcagctaa atattgcctt ttcacattgc
    23461 caagaaacca ggacatttta ggataccttg ataatttaga ttcttaccga aaacttttga
    23521 tggtcaaacc tgtaaagacc gcatctggtg tggtgataat tgctgtactg ccgaaaccgt
    23581 atgcatgtcc acagggcaga tgcatatact gtcctggcgg gatttcggct aacactcccc
    23641 taagttacac aggacgcgaa cctgcaacaa tgcttgctca aaagttcgga tacaatccat
    23701 ttgggcagat tcgatctaaa ttatcgcata tctgctccct aggccatgac gcaggcaagg
    23761 tggagcttgt catcgtaggt ggtacttttc cgtttatgcc tcatgattat caaagagaat
    23821 ttgtaaagtt gtgttttgac gcattaaatt gtgataacga taaaagcgtt agttccttaa
    23881 gcctgcatga agcgatgaaa ataaatgaga cagcaaagac tcgctgcgtc ggtctaacag
    23941 tcgagacaaa gcctgattat tgtaaggagc agcatattga tctgatgctt gagatgggtg
    24001 ttacacgtgt agaagtggga gttcagtcct tacaagaggg tgtttataag gctgtcaacc
    24061 gcgggcacga tcttggcgat gttatcgaat cgtttcaaat tgcacgcgat agcgggctta
    24121 agatcgtcgc tcatatgatg cctggattac cgggttcatc gcccgagaaa gacatacaag
    24181 actttaacac tttgttcgaa aatcctctct ttaagccaga catgttgaag atatatccta
    24241 cacttgtcct aaaagatacc cctttgtata aactctacac gactggaaaa tatgccccat
    24301 attctcagga cgagttgata gacattttga tcgcggtaaa gaaaaaggta cctccatgga
    24361 ttagaattat gcgagttcag agggagatag agccaaagga tattatcgca ggtccaaaat
    24421 tcggaaactt gagacagatt gtactacgga aattacatga ccaagggttg aggtgcaact
    24481 gcataagatg tagagaggtt ggtttaggtg aaaaaagatc aattttaatg aatgatattg
    24541 tcttgaatag gatcgattac tccgcagctc gtggaacgga agtatttctg tctttcgaga
    24601 ccagcgatca ttcaaagatt ttgggtttct tacgattgcg tcgaataaag acttctcata
    24661 gaaaggagct aaaacaagat tctacaatcg tgagggagct tcatatctac ggaccgatgt
    24721 taaatgtggg tagtagagga gaagatagat cctatcagca cagcggatac ggcactcagt
    24781 tgatgcagga agcagagagg attgcaagcc aggaattcgg agcacgaaaa ttaactgtga
    24841 taagtgcggt aggaaccaga gaatactaca agaagctcgg ataccagaac gatggtccct
    24901 atatgtcaaa ggtgctttaa acaactttaa tgactgcaga agaagaacag atcatcggaa
    24961 aaggaacttg gattgataag gtggcaagca ctttattaag acgcgaaaaa cgattgggca
    25021 ggaacttgga tatgatcgtg gtggaaagtg gacttggagc atccggcttt ccacatattg
    25081 ggagtatagg tgatgccgtg agggccttcg gagttgcact ggctcttggg aactttggtt
    25141 ataaagctga attaatcgca tactcagatg acatggacgg actcagaaaa gtacctcaag
    25201 gtttgccagt ttggcttaat gagcatcttg gtaaacccgt atcaacaata ccagatccga
    25261 tgggtagctg ccatgcatca tatggcgcac acatgagtgg tctcctactg gatgcgcttg
    25321 acagaattaa ggtaaaatat cggtttcaaa gtggcaccga agcataccac aaaggaattc
    25381 ttgtcgatca aatcgataca atactaagaa acagtgaacg cttgggcaaa aaaatatcgc
    25441 agttggttgg ccaacaaaag tatacagaaa ccattccata ttttcccata tgtaagaatt
    25501 gtgggaggct gtatgttgca aatgccgaac aatacattcc agatgacaag aaggtagtat
    25561 atacttgttc cggcagcagg attggccaac aagagattaa aggctgtgga ttcaaaggga
    25621 aagctgatat tcgcaaggga gagggaaaac tagcatggaa agtcgaattt gccgcgagat
    25681 ggcatgcact ggacgtcaga tttgaggcat acggaaagga tatcatggat tctgtgcgaa
    25741 ttaatgattg ggtggcgagt gaagttctcg gattctacca tccaatgcat gtaaagtatg
    25801 aaatgttcct agacaaagga ggcaagaaaa ttagcaagtc gtcaggtaac gtacttaccc
    25861 ctcaaatctg gctgacatac ggaacaccag aatccatatt gttattattg tttaaacgga
    25921 taaccggcac ccgccatgtc agcatcgatg acataccttc gttaatggac gaatacgatc
    25981 tatacgaaga cttgtatttc ggtaagctga aagagtcaaa tgcggctaaa ctgatgaaaa
    26041 tcaaaggcgt ctatgaatat attaaccacc tggagccgcc gcagcaacca caaccacata
    26101 caccctatcg gatgctcgta caacaggcct ccctattctc ccgagacgat gatagaacat
    26161 caaaggtatt tgcaaggcta ctgaagtacg gaatcgtaaa ggaaaaaact gaaagattat
    26221 tacaacgaat cgagctcgcg tcaaattggg cagatgaaca gttcacatta accgcggaaa
    26281 gagtcgaaat cgaaataaat gaaagtcaaa gaaaagcaat gatggagatt ttagatgcga
    26341 tgaggtcatt tattggcagc gaacaagatc cagaaacccc gaaaagtctt caatcaaaag
    26401 tttttgagat agccagaaat aatcaattag agcctaaaga attcttcagg ttactctaca
    26461 gaatactcat taacgctgac cgaggaccga gaattggaaa ctacgcaatc gatcttggct
    26521 tggatagaac atgtcgaatt cttgaggaat ctctagttaa ctgaaatatc caattttatg
    26581 ctattaatga tcgtaattaa gcatcaaaat gaggataaga ggaaatttat gtaatgactg
    26641 ttcatcctgg tgtgcgtcct gacaaacaaa aagaagaaga ggccacgggg tgaaccgcaa
    26701 tttgttttat tgtccgaacg aaaatgttaa agaaaagaga tcgcagtatt accagaggat
    26761 gaatgatctt ctgcctaaga gatcactcta ctagcctaag acgtttgcta tgaggctaat
    26821 cgctgaaaat tgtcgaagac agcaatcctg aaaagttggg tgatgttata gcaacggaca
    26881 gtggagtcag gatttttgtg gccttcttgt cgtgtcatgt cgaaggtata ctgaacgtga
    26941 atgtgcatcc tttaccgtcg gcattgtttt ctgcccaaat cgtgcctcta tgagcatcta
    27001 taatactttt cgagatgaac aatcctaacc ccgttccttt atctgacttt gtgacaaatt
    27061 ttgaaaataa acgaggcaat atttctgggt cgataccagg tcccgtgtcc ttcacactca
    27121 cgataactat atcgtccttt ccgaatgcgt taatcgaaat aaccccttct gaagtgaatt
    27181 ttatcgcatt ccttagaaga tttaaaatta cttgtgttat cctgccctta tcggcttcta
    27241 ctaagatatt tctcggagga tatgatattt tgacgtttct ggtactatcg tcattttcca
    27301 tctgttccct tagatcctgt acgcaggtcg atagaacgtc acttaaatcg aattgttctt
    27361 tatttagttt taacgattta ctttcgattc ttgtaacgtc taaaatatcc gacgcaagcc
    27421 ggtctaaccg ttttgcattg cggataatag aagcgattgt ttctttgtct acctcaacta
    27481 tatcgttagt gatatcgaat ttgtgttcga tgtactcagc atctccgaga attggcataa
    27541 taggagtacg caactcgtgg gctgcgacgt taataaactc gctttgcatc ctatcgtgaa
    27601 cctttaactg ttcattagca gaagcaagca gcttattgga ttcaatcaac gattcgttcg
    27661 cacgacgtag ttctaaggtt ctagcgtcaa ctgcgccctt taacctccta ttccaggaga
    27721 gaataagaaa ggccataccc acagctatcg ccccaatcgc cacgacggct aatgtgctaa
    27781 agttcttttg ctgatctatt agaaggccga cgttactggt tagactgtga ggtgaaccga
    27841 tatataatgt ccatagccgt tttccgtcaa tcgtaactgg ctgataggat atggtagtag
    27901 tgttaccacg aactgttatg tcttctgctc cggccttacc ttgtaatgag cgtcctaaaa
    27961 taccgtcgta ggaacccttt atttcaggag gaactgagga ttggaattca tctcctaagt
    28021 agtttttgcc tattagagca tgatttctag cgtacaagaa cacgccattc ttgtccatta
    28081 atcctacatt gcttataatt tccggggata gttcactctg taatgacttg cctattgaat
    28141 taacgttcac cgctgcgact ataactcctt tgaatgtctt tgtcacgttg atgctgccgc
    28201 ctttatcaaa tttggcttct ggcaccatga ttgggaaagc gatgtacaac ctcggaacat
    28261 tgtcagccga tgtcatcacg cttgtataat atggagaatg tgttttcttt ggaacaatga
    28321 aatattctag agagcctaag tcaagactct tagactttcc aaccgaagaa ccatttgaat
    28381 tactccatgc gattagtctg ccattttgat ctagcaagta atatccgtca gtcagtacct
    28441 tcgtggaagc ctgtgcactg tcaatcaaaa cctgggttag tgtagtcatg ttgttgtgga
    28501 tagcaggagc ggctgcaagt gttcttaggt taatagtaat tgattgtata tcatgtatca
    28561 ataactcaga aagatcatgt gcttcaattc tagcgttgga tcttatctct tgtgaggcta
    28621 tttcagcgat gtccttagct gtgaccgagg aatattgata cgacagaatt gataaagatg
    28681 ttccgatagc agcaattacc aagagtaaaa gaatattgct tttggaaaat gctaccattt
    28741 ttcaccgcaa atcattattt gattgctctt ataaaaacgt caactagcta atgatctgac
    28801 cagaacttgt gaagcgctcg gaaatctcgc cataagaaag cagnnnnnnn nnnnnnnnnn
    28861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    29041 gcgtactgtg ggttgtgatc cacagtgcgg aatattagct atgtgtctat cacatacgat
    29101 gtcactggcc gatagaatta caatcacagg ttggcgattt ataatcggtg ggatagtctc
    29161 aattgttatt ggcctaatct caagacaaga tggactataa cggcagcgtc tagggtgttc
    29221 tgaaaaagcc tttttgtaat acggtaatta attgactgtg tcctagccta caataataac
    29281 tataattggg ctgattgaat gcatgacata tcgttcactg gatgcttttc tttattagca
    29341 atcacacgta acatatgtcg gtgactatct agcatcgtat aatgatccgg tcatttctga
    29401 tactaattca tagtaacatg ttcacagtat tgatatcaat aattgttatg ataatctaga
    29461 atagagaaaa atatctaaat gaaagaatta atcagaatat tgaaaaaaga ataccataat
    29521 agtatttagt gcggctttta ttcgaagaga ttattatgtt atgcaagtca tgaaacacag
    29581 aagtagacta gaaataattg ccgaaatcct cgaaactgcc aattcgagta aatctataca
    29641 agcaaaaatt atgcacaggg tatatttatc ttatccacag cttaaagaat acttagcgct
    29701 cttgctcgaa aatggtttaa tagaatacag acaaacagac cgaatttacc ggacgacaag
    29761 tagaggaatg cgtttcttgg aaatttatag acagctaaat gatgtcgttg ttatgaccga
    29821 caatataata gtaaggtgaa caacaaatag cttggaatta gtcgtaacgc gtaggtcttt
    29881 ctctagacct cccgccaaat tgcctttgtt ttagccagca taacaaagcc gatcaagtac
    29941 gagacttgca ctaacaggcc aagagcaatt gaccattctt tcggagttgg aagccccata
    30001 gtgtactgta acatcaggga agcgtgagtt gtcgggacga tatatgccag atactgtagg
    30061 tccaacggta gcctaccgat cgaataaaag acaggcggta acacagctaa tatgacattt
    30121 acaaatgata taacctgagt tgcatttcgc atatgcagca tatgggacga aagaaaaaag
    30181 cccatggcag acatcgatcc ccagatcaag aaaatagttg agatcagtag cggtatgtta
    30241 ctaatcgagg ttccgaagaa agcagctagt acggtaagca cagccaacgc cggaaaccca
    30301 aatagcagtt gcgacaatgc cagccctgtc atgtatgtca atggagaaac cggcgatgcg
    30361 acgaagacat cttggatttt gtattcagtc ttgtaaaaag atatgtcttg tccaagagct
    30421 agcccataac ctactaatgc cataacaagg ctcccggcga cggcaaactg gatgtaatgt
    30481 ccattgctaa ccacgaataa aacaaataat agcgaaaagg ggcttattgc agaaaagacc
    30541 atagatattg gattcctccg cagccagagg attcctgtca aatatgcaat caaaagtact
    30601 tgcttaagaa ttaagaatct tttcgattct ttaactctgg ttattatcat ataatcgatg
    30661 gcaacacgat tttttatgtt ttaggttccg cggatcgttg ttctgccatc tctcaaaagt
    30721 gaatgaattg tcacttaatc tctaatctcc aggacagaca tcgggtttag agagttaagc
    30781 tctcctcttg ttggttattt tgtgtcatgg ataatatgtc aattccatcg ccgcttgcag
    30841 agtctctttg aatagatgat ttaattgcct taattgctag ttcttttgca ctctgttctg
    30901 acatgttagc cctgaattca ccatccataa ccccaagtgc catttccgca cctgatccca
    30961 cggcggcgta ctcatcagga agcacagaac caagaggatc caacgtatag atttgcggtt
    31021 tgttgtctat tcctcctact atcacctgcg tcaataacgg aaagaatctc cgttcgaaca
    31081 ttatcacaga catcatcttt gcaatagaat ttggaggcac actacgtcta gtttcgagct
    31141 ttctaatctt ggcaagcgct cccacctgtc ttaccaaaat ttgcatatca gcaatcatac
    31201 cggcacatgc cgctccgacc ttatcagtga ccggaaaagt tttctttgta ttcttgttta
    31261 ccacgaagtt cccgtatgaa acgcgttttt ctgatgccaa tacaaccccg ttgtcgaatg
    31321 agattccgac agcggttgca cctggcataa attggaatgc cattatagtc ttggatatat
    31381 ccaatgtggg gcagatttag gcgtttctat caattaatca tacttcatga gcgcgtgtgc
    31441 taaaccccgg aatcggaacc gatctaaaag aatacctaag gcatactcaa aggtgaaaaa
    31501 gcttagaaat tatggagtga atcatttgat tttcattttc ttgtgagagc acggtcatgg
    31561 attcttatcg cttcggcaac gagcctgttc tgaagttcaa gggcatcttt gccctctgga
    31621 tgttttgttt ctgccaatgc ctctgccaca atactaataa tatgccttaa aaggaactcc
    31681 tcccctcgcg tagattctgc tttgtaggtt gagttcgcct tatttacaaa tactacattc
    31741 tcattcttga agctgtatct agaatcatat tcatcctcat atgggataac cttatagcca
    31801 acttttttta acgcaaatgt tcttttcagt attggtttct taatctgttt ggttatcacc
    31861 gtattgacag gagtagtgac tgccacaacc tgttctggtt gtaagagatc caaattcgtc
    31921 ttatcagttg ctgaaagtga acctacgtaa ggagttggtt gctgaatctg gcgcggttgg
    31981 gtgatagtcg tttcggatac gggaggtgac tcaattttaa tgcccccatg tggctgtgct
    32041 tccttggata cgatgaaaga tgtattctca tcgctggaaa tatttgcaaa aagggcggca
    32101 gatgattcac cctcctctat cggtgccttt tcattgacat caattatctc atatccatct
    32161 acctcttgtt gagtttccag attttcaaca atggcctgac cgagcacttt atcgatctcc
    32221 ctgtagatct tcgattcttc tcgtgtaatc aaaacgtctt catactctgt taaactaggc
    32281 aacactgttt ctatgataaa agctctcatt gcctggatga attttgcgta ttcttcgtct
    32341 tcgattatcg cggatttgtc agcaaacctt gacgttaatg tatcacacct cacaaaccct
    32401 gtcaccttgc ttaatttagt atcaaagcca aaattaccgc gcgttactat gtgatttccg
    32461 accataatgg ctattccctt ttcatcctca gtcaaaggtc ttctggcaat aacgagttca
    32521 ccggttattc gaccgtcttc tagatttaac atgatcctat gcccctgtat ttccggggcg
    32581 attatctgct gcgcaccact aagatcccat tcagcaaatt tttctgcctc cttgatatat
    32641 acttcgaaca taggttgtct taggattgcg agtttacgga tttcttttat cagtctgaat
    32701 gaatcaataa ctgttttcgg acctttaagt gttagctccg taccgttacg gcctagggac
    32761 gactcgtgta attcgtccaa tagagcattt ccgctaaaaa gcttgtcgag agtgttacca
    32821 tctatcacga tagtcttctc aaacttatgt cgttttgtcc tgatcttcat actttcgaac
    32881 gaagtaagaa acgatagtct cccggttccg tatcttcccg tcctgactcg gtttaatctc
    32941 ggtgagattc tatcatgttt tttgtgagga gatcctaaga gcaagaattt ttccatacta
    33001 tcctggtcca tacctgcgtt atcctcgatc acaactgagc cgtctttcat tatcgtcaca
    33061 acggaaatcg tagcatcctc gtcaaatgca ttttcgatta gttccttagc aatctcattt
    33121 ggcgaaaccc aagtttttgt agcgaattct ctgaaaaatg atggatgtac tcttaattca
    33181 gttgtcatta cttgtttgac gggtacttac ttacctttat ttattacgtg taaaattatt
    33241 attgacaacc tactcaggca acgtgttcgt aaagcttgta cagcatttcc caaaagttca
    33301 agggtcagaa aattcctaac ccagtagata ataactgtcc tatttgttca tatcagatta
    33361 ctagcggtct cgtttgcctt cgataaattc taagaaattg gtcttcgctt gtgaatacga
    33421 catttgtact gggggatgtt taaaacagta cgcacttata ctttcgagag gcccgctgat
    33481 agacctatcc agagctaatt tggctcctct aatggcatcg atcatgactc ccgcgctatt
    33541 gtaagcatct accacccgca gcttaagatc cagttctatc ggtgtgttac caaaatacct
    33601 cccctttaat gagatataac ataccttatc gttttctaaa aaccgaacaa aatctgacgg
    33661 tcccacacgc attggaatct cgtaaggcgc catcgctgct accgcccttg tcttactgat
    33721 ccttttatcc tcgagtcttt cgtcgtcgag catgttgtaa aagtccatgt ctcctcccat
    33781 attcagctga taggtctcat ctatctttac acctctatcg acgaataact ttacaagtgt
    33841 tttgtggagg acggtcgcac ccaattgact cattacatcg tctcctgcac agggcagatt
    33901 cttattctgg aaggcattct gccattgtgg agtggatgca atgaagacag gtatcgcatt
    33961 aatgaaacag actccagcat ctagacacct cttagcataa tattgagtag cttccctgct
    34021 cccaaccggc aaataattga gaagtatttc ggttttagtg tccctgagga catctgccac
    34081 atcagcagcc ggcttatcag aaattctgag cttttgactc agatgcttgc ccactccgtc
    34141 gaaaacctta cattttgtaa ctttgacatc cattttgtcg actttggtaa ttgtgacagt
    34201 attatttggt ttagcaaaaa ttgcctcaga aaggtctttt cccacctttg aattagtaat
    34261 gtcaaatgct gcaacaaatt caatatcacc tggttgaatt cccccaagcg aatatgccgt
    34321 gagacctatc ccatgattat tctcagtagc agaagagtaa tatcgtgtcc cttgtacaag
    34381 agcagacgca caattcccga ttcccgctag tgctactcga actttttttc gcatttacag
    34441 ttagtgtatc gctgtaggca gcttaatatc tttatcaaga atcgtgatcg gactggcact
    34501 caatcggaat ttcttttcaa agcatatact cttttcttgt aaacgaagcg aaatcctaat
    34561 tttttgtaca atgggaacaa tccttcattt gtgaacgtct ggagaaacag aaggtccaac
    34621 ccctgctgct gagctattcg tagcgagacg cctatcattt tcttcgcaac tccacggcct
    34681 ctaaaatttg aaattgttcc gaggcaatac agacccatga gcccctgatt aggaaataga
    34741 gcagtacacc cagccggttt ggcagaagaa tcgctgacac gaaaataaga cagcagtaaa
    34801 atcatcttct taaaatgcag atcgattttt tcttttacct ctgatttcca ttccaataca
    34861 tcgaacgatc tgcagaacgc gtccacccaa cttggaatag accttaaatc aacacgaacc
    34921 acatggattt tctcgttatc atattcaact cgctccgact ccgctcttag agtttgcatc
    34981 gtgtctatcc acttgaagcc tgctgttgat agacagtttt ggacatctgt attcttatcg
    35041 tgaatataca catagcagtc catacccttt ctcaaacaca cattggctgc ttcctcaata
    35101 acattcgtag catcttcgca gacgaatggg ttcagccgat taaagaaata atctcccact
    35161 agtttttcat tcacaaatag gtcagcacaa tcacgaattc taatatgcac tgtccacaat
    35221 gaacaaaatg ctgtttcgtt agcctccatc cgcttaataa taaacaagtc caaaagtccc
    35281 tactatggac cgcaagtctg tgagggaatg ccgacttctc gcggtctcca tcgttaattc
    35341 caaagcagga ggtgtattta catcatcgtc tatataactc gtgaattcat ttattagttt
    35401 tgtctcaaaa tcaggttttt tactaatagc attgccccaa agtgcacatg caattgtttc
    35461 gtcaatggaa cattatctat gaagttcagg ttgtgagaac actaaaggtt ctctataatg
    35521 cttcgaaaaa agatacagcc ttaaaatatt tgagttttat ttttttagtg atgtctctta
    35581 ttcttagggt gttaccgaat gatttataca tttttcgcct ttttatgttt actaatgcta
    35641 catgattcca aatcgtgact ggttgttgat tagatgttaa aacctacaaa tgcttgttgt
    35701 tgttcaagcg cggaagcagg atctacaacg actattagta taacaaagat tgcagtaatt
    35761 gctaatcttc cagtattcac cagtttctca cagataattt ggtatatctg aagtgcttcg
    35821 caagaactgc taagacggaa aattgtggat atatttagaa aatcgttatt ttcattagaa
    35881 agatcaaagt taaattcgtt ggtctgcgta ctaaaggaga taataatatg ggaaagacag
    35941 ctatattttt ctaattaaaa atgtcttaaa cagtttacga tactcaggaa ttggtatcgc
    36001 aaatgtccga ctgttaaatt cagccactta aatcagatgt ggattggtaa cctgaccaac
    36061 caaccggtaa tattacctac catgtagaat cgtcccaaca gatatcatga tcgctcgtgc
    36121 ttgctgacgg ctgcagcttg ttgataatat gaacagcctg aataatagta ctcataatat
    36181 aaaatatttc tattcccctg ctgaagtttt gaaattcgta aaatctacat tcaataaaat
    36241 tttctctatc tgcctatact aggacatatg tactatccta gattataata gagtagattt
    36301 gtcgttttag ctccgctcat tatcgtcaat caggtccaag aaaggctgtt aannnnnnnn
    36361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctatgtatgt tatcttgcgc
    36661 gtgaaatcga gacttctatg gtagaaacat ttcgcagcct tccgtccgga gactgcaccg
    36721 gttcagatcc tatctttacg gggccaattt tgtatcctag catgtccatc ctcttgacga
    36781 ttatctgtgc gacgtccacg gctctggtaa tactctttcc tcgagccttt ataatcacga
    36841 tcggctcatt agccagttga actagcgtag ccgtaacata agtcatcaga ggttttttac
    36901 ctacaaaaat ctcattgctt gctttatgtc cagtaggtgg gttgctagtt gacattggtt
    36961 taggatttat cccttgcgct ggaatataat gtttgttgat tgataacatg ttattcgttc
    37021 tgagaatatt ttttcaatat tgtttcaatg tcagatgggt ttgcagaaat ttctcttaca
    37081 gcgtcagcgt tttcgacttc atagcagtga agcagttctt cttcgtcttt gtagaataga
    37141 aaaactttgt cttcaaaaag acaatgatat aactgattaa tgatcatctt ttctggtttc
    37201 aatttaacgc catccgactg aaccgtaaca ggatattcca acttgaagag agtatagcaa
    37261 catctgaaaa caaaaactta tctaaaagca gtagaggaat tacctccaac aacgtcccct
    37321 catttcattt ttcggggggt ggattgcccc gatttagttc tgtatgtttc attattcata
    37381 aggtctatta aatcgcttaa ggaatgtaaa acgaactacc ttgaacgtaa ccaagagagt
    37441 atatttattt ttctaatgtg ggtgaatagc agctctgaaa tgcatgtcct gaactatcaa
    37501 aaacataggg ctctgtcgat aatatcacat ctaaccgaaa ctgtaaaatt aattctggat
    37561 ccgttgagtg gaagagcgag aaccgtagct aaaatatcga aaccagacta gttaaagtca
    37621 gagcgccgag gataaagtgt ctgatttgaa gagtaactca gatcaaactg cgatactgga
    37681 tctcattata tatctattcg acaggtctgt tgtcttgacg acattatact acggtgatcc
    37741 cgtcgactct gaagaagcat catcagcagc cgaagcagct gagatactcg aggtgttgca
    37801 tagctgtgcc tcatttctgg cccttttact ctcaagagaa acgacactag caaggtagat
    37861 ccccaaccag ggaagaaaca taatctcgcc tgcaagggaa tgaaatgtct cccattccgc
    37921 aacattggta gttattatca acgcaaacaa tgacaacgat attatgcgta ttacattaac
    37981 agaagcagtt cctataactc caagcccaaa gtagacgagt ttcctcccga ttgggatatc
    38041 catcttgaga aggaaggcaa gcatcactag actgtaaata atcatactgt ggacgccagc
    38101 agatggccag aataccttta gcgcaaaatg tccatgcaag ccgtttaaca ttaggagatt
    38161 accttgcgcg gtggccggaa cgcctggccc cagactcatc aaatggctat caatgagtct
    38221 aatgacccac tgatctatct gcaggtaaga agggacaatg atttgtagcg gtcccagact
    38281 gtcatatggg aagaatgcat caagcgaaag aatcaaggca ctacccgcta gataaatcgc
    38341 ccctgcggaa gcgattttat gccatttttt tccaaataat acaatctgcg acgaaagcac
    38401 gtagatagcc attactagaa agtcccacat ccaagtccaa ctatcgacaa gttggacctt
    38461 gtagaatggc gcggcggata ttatcgaatc tctaagtccc aggggcaatg caataaaata
    38521 tccaattgta agagcagata ggcagatcac aaggtagtat ttttttctat aaacattgag
    38581 ttggagtccc catatttctg ctgaaatgaa ggccagagcg aataagaatc ctccccgacc
    38641 ttcattccat gacaggttaa acgtactagg ataggcgatc atcgaaaaga gaatgggtgc
    38701 aactagaacg agtatgacgg ctaagtttgg acttagccca aaaatttggt tgctcttacc
    38761 accgcaaagt gcccgtttgc ttttatcgaa caagtcctgg ttctagtgtt cattagtcga
    38821 aaataaattt gttctacaga agagtttctg cgtggtgttt atagacagca actattatac
    38881 caatttgtct atcttgataa taagcagaat tgaaatctag aaaattaagt ggagattcta
    38941 cttctattac tattcatata agactgcatc catttttcat ggcatacaac gtattgaacg
    39001 aacttttttc gaaacttggc cgcaggtatt taccctgtgt attgtcccac tctgaattaa
    39061 aggctacacc agctgtttct tatcctgatc tatctacgct gataatcagg ttgcctgact
    39121 cactgaaact tattaaaatt atcgactact tctacggata ctggcacgcg cagcaagttt
    39181 tgctattctg atcggctctg gaatagaacc ttggagggta aacgaattaa ctatcgcgga
    39241 ggcttctttt gagcttagcc cccagtaccg cagaaagaca aacttaccag tctttaagag
    39301 aatttgatct ctctgtccta ggttacgata ttgttctaat tttggcttcg agtcgttggg
    39361 aaaatgacgt tgtatagtcc cttcaagtcc ttccgagtcc ttgaatgtta tcgcaataac
    39421 tgggacaccg gtcttttctc taatttcttc cccgtgtatt atgttgtaca tgctaataat
    39481 aaggccgtga agcattatg
//
LOCUS       DAOR01000193           15682 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_14356, whole genome shotgun sequence.
ACCESSION   DAOR01000193 DAOR01000000
VERSION     DAOR01000193.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15682)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_14356"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         4787 a         2996 c         3364 g         4535 t
ORIGIN      
        1 aggaaactac tacattaaag gtgaagtggt taacaatggc aatgccacta tgcaaaacct
       61 gaagataacg gcgcattgat atgattcaac acataaactg attggagtaa cttacggtta
      121 cacagacagt ctacagtccc taaatacagg aaagagagca accttcacaa tacttggaaa
      181 cggacccagc gatttgctag gtaaaccaaa attcgtggag ttgggttatg actggcaatg
      241 atgtttcgta ttaagaaatg aaacagcatt ttgaagtaaa ataagttgcg gaaatcatag
      301 aaatgagctg taaaggtatc tgtatccacc acaaagctgt taaaggagtt cgtgggtatc
      361 gttatcttaa tggagagaaa agatgccgtg tatgttgtat atttttagtg tgggataatg
      421 atttttgtcc aggattaggc cacgcagttc aagaggtaag gctatgtggc aagaaatgac
      481 tattgcgaca gcgatttaat atttggacga gacacagaaa tgactattta tgcgttatgc
      541 cgactaaaag tcagccattt tggctacagt ccaatatatg ccaggagatg aaacttctca
      601 aaggtattat tatttccaga taagtggacg gaatcttcaa gagattgtta gacgaatgaa
      661 taagcttaaa ggagaactcg atacgcatct tgaatgtaat cccgctaata gctcaatacg
      721 actcgatttc gaaaacgtgc ctagcggtta gttaactttt tattttcctt aacgccacta
      781 atcaatagag catatagaat tatatgatct gtttccgaac atatttttga ggaaataaat
      841 cagtaaatga ctaaatatgt agtttcaaat actataaatt taggaatttt tgtatgaaac
      901 caaaagatgt ctacaacagc gtgcctatca cataaagagg atgtcgatgg tatattatct
      961 gctgtattga tcaaggctgc gtttaatgct aaaactatca tcttggtcga ttaccctaat
     1021 attatctcta ccctcgataa actggctgct gctgactttt tacctaaact tgatcgaatt
     1081 tttgttagcg acttgggttt gagtaaaaaa aatgagggag aatttgtaga tatagtcggt
     1141 aaaattatct caaatggtgc taaggtcaca tatattgatc atcatgacat aaatcgagag
     1201 agcattatcg cactaaagaa aataggagta ttgctgattc actcagttga agaatgcagc
     1261 agtgtacaaa tatacaataa attcaaaaag aaattaaagc cacatgctgc tttttttgct
     1321 gctgctagtt cattgaccga ctatatggaa aataagccta tcgcttctgc catcgtatcg
     1381 cgatatgata ggcaatttct aatgttggaa tctactgtgc tttcctatat gatctcggct
     1441 aaacagcatg acgacgagtt tctaattgag cttgtcaatt tcctttcagg tatgaaatat
     1501 cctcacgata tagaaggtgg tttcaccata gcagaaagat acgcccgaaa ggtttccgat
     1561 gctattaaga aaatagaagg atctataact aaatccgata acattgcgta tgcgcaaaat
     1621 aattcggcac tatctgccag tatgattgtc aactttgttt tgggtatctc agaaaagcca
     1681 gttgcaatgg tctataagtt aaaagacgaa attgattcct atattttttc tatacgtggc
     1741 aacaaatcct gtaaagtgca tctaggaaga gtggtaaatc agattagttc agaaataggt
     1801 ggaagcggcg gaggacacga aaaagcatgt ggggcagtgg tcccaagaga aaaactcaat
     1861 caattcatat atcttttgga tcgagttgtt gcacagtaat aaagaccctt gatctttact
     1921 gcattattat ttagaattgg attaccagtg ctgctaacct aaacctaact aacttaaagc
     1981 gtccggagtg tggtaaagga ggaggtgggc ggtatcaaaa ctggagcaca acttataaaa
     2041 tgcgacagat atatacagtt agtatgggcg gcagtaagaa aaaaccgttg ggatctgaaa
     2101 aatcttctcc agcctctggt catgacggag ataaacctgc aaaaaaggaa gagaccaaga
     2161 aggctttagg aaaagtccag caaaaacaaa agctctcggt tttggtggag gatacacaag
     2221 gtaggagagc tatacagagt atgaaagcta ttacgattca aggtgtagca agagctgcgg
     2281 gcgtaaaaat ttctgtagct aacgcatata tcaaatcact cgaagcaaag ggcatagtta
     2341 aaagtgttgg tggttatagc ggccacagag tataccagtt gattgagcag gtttaattaa
     2401 tatgccgtag tatcattacg gctcctgttt gcgccgattc tgctaaatgc aggttggatt
     2461 taactacccc tatcggattc atagcagata ttggtgcaga atcattaacg aaaatacata
     2521 cggaaccgtt agacgttaag taggccatct ctccacgttt aaatcgagat cgttgtttct
     2581 ctgctccgat cgttaaccct gtctctatat atactaaatt ttctcccagt ctatgaatcc
     2641 tgtccttcag aggcaaacct cttaaaataa tcttagctgt gatcggggaa agatggcgaa
     2701 taatttcgca ctcgatgact cccttatttg atatctctat aataagaggt atccttgaaa
     2761 ccgtgctgaa cttatttcac cttaacccaa actgcctgaa atgggaagat ttaattgact
     2821 ggacattgaa aaattcgcaa catttaatta tataattgtt atcctttttt ttcttggtgg
     2881 ttaaacattc ttcaaatcaa aagacctctt ccaaaaagtt taaaactaaa acttctaagg
     2941 ggacagacga tactacgaaa aagagccgta gccggcgaga tgtccccacg gccttgaaaa
     3001 aaatagccgt caaacatgat gctacactcg aaaaagctca ggtacaatta gaagatactc
     3061 cagtcaagaa atcgagccgc cctgaagatg aagttgtgac ctatgctgaa gttgaattcg
     3121 cactcaagga gattatccct aatggcactc aagttttagt cggaccggca cgcattacga
     3181 ggtttgaaaa ggcaaggatc acaggtgcca ggtctttaca gctttctatg ggagcgccgg
     3241 cgtttattag agtaccccaa gaggtaagag attcaatctc tctagcaaca gcggaattat
     3301 atgctaaagc attgcccata tctattagac gcgttctacc taacggtgta taccaagata
     3361 tcccgataga ttggttgaat tgagagatgt ctagaatatg atagaattca gttacatcaa
     3421 tgaattctca tagttatggt aaaagtgatc attgctcgac ctttattaga cctctcattt
     3481 ccggatgtat gttgcagtag taaacaaatg tgccaatttt atttgcaatg aacgtgaggg
     3541 attgactttc aaaatacccc aggcttttgg tatgtacgtt aaatgtatct atattcaggt
     3601 tatggagtga gcgtgtatct gcatcctcat tgattaaatg gatcgagtca agcgcaccat
     3661 actttaagaa aatcgtcggg ctgttaaatc ctgctcccat gccattactt cgtgaagcgg
     3721 cggtagattg gctggtgaac agccagcccg tttgaggaga atgacttgct ttcataaatg
     3781 tatttatcgg aggagcaaaa gccggagtat tcccattcac agggatcaaa gaatgtattc
     3841 caaaatagcc ggctattacg acaactactg cgatacctac aaaaatcatt atggcatatt
     3901 tatatttgga aaatgtaaac ttgctattct tgacgttctg ttgccgcttg gctttttttg
     3961 gcatccaagt gggtgaatca agctagtata tatttagtta atgatcgaac gctccgccaa
     4021 gccccagtcc caattaccag ggagataagg atctgacaag tgatgtcagc gaattgagtt
     4081 tgcccactga tgcaagacag attctatgac atggtttatc ggacaaatgt tgcgaacgat
     4141 gggacactat agcaacgaaa tacaaatcaa gagcaaaact taaatttgta gtcccgaaaa
     4201 gttgctaacg atcaaaaatt ccaacacttc gtcggttgta ctttggcgac caaggcaagc
     4261 tcatgataga ggacggcagc atattcgacg ggattggttt cgggtatcct gctgatattg
     4321 ctggagaagt cgtttttaac acgggtatgg ttggatacac ggaaacactt acagaccctt
     4381 catatcgcgg ccaaatatta tgtctaactt atccactaat tggaaattat ggtgttccga
     4441 gtatcgaaga cactgacgaa tttggattgc ctaggttctt tgaatctgat agaatccaaa
     4501 tcagaggctt aataattcaa gacctatcgg tcgtagcaag tcactggagt tgtgtcaaaa
     4561 cgcttgatga atggctatac gaccaaagga ttccaggaat atgtaatata gatactcgag
     4621 aacttacaaa gaaactgaga attaacggag tgatgatggg tgctatcagt acttgggctg
     4681 tccaagaagg cgtggcggct aactcagaaa ttgacgtacg aaaaagattg tctggcctta
     4741 taaaaaacca gccgtatggt caacaaaatt ttatgcctga agtttcgaca agtattcccc
     4801 gcgtatatgg cgataaagca agggataccc ttgtactatt ggataccggc acaaaaaaca
     4861 gcattatacg taatattctc aggataggtt tcagggtcgt gcgtctcccg tgggattcat
     4921 cttttgaaga agtgatgttg cacaaaccaa agggcatagt aattagcaat ggtcctgggg
     4981 atccaaaagt ttgcaaaact gcaatcaaaa ctgcaggcga gcttatcagg gcatcagtcc
     5041 cgactttggg aatttgtctt ggacaccaga ttctagcatt ggctgcagga gctgatacct
     5101 acaaattaaa attcggtcat cgaggacaga ataaaccttg catagattta cgaagtgatc
     5161 gtgcttacat cactagccag aatcatggtt atggaattga gcctaagagc ctaaacggta
     5221 ctggctttaa agtatggttt tcaaatgtag atgaccacac agtagaggga attgagcatg
     5281 aatctaaacc gataattgcg gtccaatttc atccagaagc gtctccagga ccttatgact
     5341 gtatatttgt ttttgataga ttcaaagaat taattgacga tttcaacaga aggtcatcat
     5401 ctgcccgaac aactgaaagt aacaatgaga ataacaataa taatccaaaa gtgaagatgt
     5461 ctgcggcgag gaagaaaaaa accagtaaag aggaggaaat ttgccaaaac atgaaacaat
     5521 aaaaaaagtt cttgttctcg gaagcggggc aattaaaatc ggagaagcag gcgaaagtca
     5581 gacattctga ccgttatttt gactactccg gttcacaatg ccttaaggct ttaaaagaag
     5641 atggcatcaa aagcgtccta attaatccca atattgcgac cattcagaca gacacaaggt
     5701 tcgcaaacag agtataccta ctaccgatta acgccagcta cgttgaaaaa gtcattgaag
     5761 ctgagctgcc agattccatt atgctaggct tcggaggaca gactgcactc aattgcggcg
     5821 ttgcgattca caagcttgga atactgaaaa aatatgggat caacattctg ggtacttcaa
     5881 taaagggcat cgagataact gaggataggc agcaattcaa ggacgccatg ataagaagta
     5941 gtgtgcctgt tttacctagc gccgctgcgt atactttaga aacggcatcc aaagtttcca
     6001 gagcgattgg ttatccagtt atcgttcgag tagcttatac gctagctggg cgagggggtg
     6061 gtgttgctta caatgagtac gaccttcaag aaattgtacg gcgtggatta tcactcagta
     6121 tggttggaca agtcctaatc gagaggtatg tcggagactg gaaacaaatt gaatacgaag
     6181 taatgcgaga tggaaaagga aatagtgtta ttgtttgcaa tatggaaaac atccttggaa
     6241 tgcgcgtgca tactggagat aatatcgtcg tagcgccatc gcaaaccttg aataattctg
     6301 aataccattt gttacgctcg gctgctctgc gtgcgacggc ttattgtggg gttgtgggag
     6361 aatgcaacgt tcagttcgga cttgatccga cgtctccaaa gtactgcgtt atagaaataa
     6421 acgcaagact ttcgagatct tccgcgctcg ccagtaaagc gacaggatac ccacttgcat
     6481 atatggctgc aaagatttca ctgggatatt ctctaccgga gttgactaac agtattacca
     6541 gggcgacaac tgcttgtttt gaaccttcat tagactacgt agtcctcaaa atgcctcggt
     6601 gggattttcg taaatttgag ctcgtgaaac gtaaactcgg aagcacaatg aaatcagtcg
     6661 gtgaagtgat ggccatcgga cgaagtttcg aggaaatctt acaaaaagca attaggatgt
     6721 ctgaaatagg aaaagatggc ctagtagcca atcctgttga atacaaaaaa agtcagaact
     6781 atgtttcagc cgaaatctcg atgatagaac aaattgggtc agccgcaggc agacccaaca
     6841 tgccaaactt tccagcgcag ggagatggag aagaggatga aacacaggaa gggggggaag
     6901 gtagtacgga gtatacagaa acgatggaac aagcattgct ccatccaact gacgaaatta
     6961 tatttgacgt tgtcaaagcg atgaaagctg ggatgacttt acaaaaaata accatgctat
     7021 cagcgataga tccatggttt ttgatccgaa ttaaaaacat aattgatatg gagtatagat
     7081 tacgacaaca atctgacttt gatgaagata ttatcaaaga agcaaagaaa attggttttg
     7141 cagataagca aataggaaga tgtctaggca tcgacgaact aaagataagg agtttgagac
     7201 accgatttgg aatcagacct gtggtaaagc agatcgatac actagctgcc gagtggcctg
     7261 caaaaacaaa ctacctatat ttgacctatg gtggccagaa tgatgacatc ctggtggaaa
     7321 cgggatctaa gagaagcgtc atcgtacttg gcgccggccc ctatcggatc gggagtagcg
     7381 tggaatttga ttggggaaca gtaaacacag tctggggtct aaaagaaaat ggcgtcccag
     7441 atgtttcaat aattaattgc aatcctgaaa ccgtctctac agattatgac atttctgata
     7501 gattatactt tgaagagttg acctttgaaa gaataatgga catttttgaa aaagagaaac
     7561 cagttgggat agtaacttgt gtaggaggac agacggctaa taacgttgct cccaagctag
     7621 ccaaacaagg aattaatata gttggtacga ggagccagga cgtcgataga gctgaggaca
     7681 gagaaaaatt tggaaaatta ttggattcat tgaacattaa gcaacccgca tggaaaaaat
     7741 ttactagcat atcaaaagcc aaacaatttg cagaaactgt cggctatcct gttttagtta
     7801 gaccgtcata tgttctcagc ggcgtagcca tgaaaattgt ttgggcggaa caacagttag
     7861 agcaattctt gacagatgct gtaagagtta gtcctgatta cccaatagtt atcagcaaat
     7921 ttttacagga cgcctcggag gtggaagtgg atgctgtagg aggtaccaat gaagttctga
     7981 taggttctat tatagaacac attgataatg cgggtataca ttctggagat gctctcatgt
     8041 gtataccccc gtggagactt gaccgaaaaa ctactgagac attagtggat tattcaagtc
     8101 gaataggtag agcgttgaat gttaggggtc cttttaacat tcaatatctc atcaaggacg
     8161 atgaagtatt ggtaatagaa gcaaatattc gagcgtcaag atcaatgccc tttgtgtcaa
     8221 aatttgtagg aataaatcta ataaatattg ctgcaagggc gatgactggc agggctcttc
     8281 cgaaaaacgc gaaagatctc tggttaaaga cttcaggatt cggaatcaaa gtcccacaat
     8341 tttcttttat gcaacttgaa ggagctgaca tagtgcttag cgtagaaatg caatctaccg
     8401 gcgaagtagc atgctttggg aacagttttt acgatgcatt gtctaaggcg tataccgccg
     8461 caggatacgc acttccgtta tcgggttcag cactacttac tatcgggggc cagaaaaata
     8521 aggaaaaatt gttgccgttg atttcactta tttcactaat gggatacaat ataatggcaa
     8581 cagagcatac agctgaattc ctgctaaata atgacttcaa aaatatccag gaggtttaca
     8641 agattagcga gccagaccga aaacccaata tttcagattt gctgtttgaa cacaaaatta
     8701 attttattat aaatatcccg tcaacatcta caattgagaa gtatgttgga atgttgtatg
     8761 acgaatatca aatcagaaga aaagctgttg aaatgggtgt ccctgtccta actacgttgg
     8821 aagcagcgaa ttcgttcatc aaaactctcg aatggttaat gacaaatact ccaacattga
     8881 cttttatgcg ggattatgtt aaattatgaa cagtagcagc cattgaaatt acatctcgtt
     8941 attcttacca ttagtacaaa acaggttcat gataatagcc ttgccaccac gttaccgtca
     9001 tcagcaacta ttgaaccatc cagtgtgaca gccagtgcct gttcaatcgc gttatactga
     9061 agtgccctgt tgattattgg cgaaattgat ttagcgtacc gtttatcaat catcgtatgt
     9121 acgtccttat taaatgaggg cacgattatg atgtcgagcg ttcctacact tccagggaat
     9181 atcatctttc tcttcacttt aagatacagc caaatccgtc gcccgttgag aacgctattg
     9241 catctcgaga aaaccggatg gatatgcccc attacaattc tttttatcga tgctctagca
     9301 gaggatggca tcgtatggcc gtgcactaac aaggtctccc ccacgaccat gcctttgacg
     9361 ctcataatat taatattctg tgggattaag aaccgaacgt aggtatcatg attacctggt
     9421 actacgtaaa tgctcgcgtg tccagagagt attctgaaga attccgggac ttggttccat
     9481 tcttgtttgc ttatggatcg tatggtgctc tttacatcac cgagtaatat tgcgccgtct
     9541 aactgatttg atttgataag gtcacgtaac tcttcaagca tcgtattgag agaatatttc
     9601 gagtcaacgg tgactcctcg taggttcagc tcaccctcaa atccgatatg taagtcggat
     9661 accaccagat atgtgcgttt tatcccagtc ctttcctctt cgagtagtag tgctgggtga
     9721 ggatacaacg gccgaataga aaccacgcct aatcactgtc tctttctttc ttgtttgatg
     9781 atcgtaacgt accaaaaatt aacctataat gagaatttga aattactatt ttattagaat
     9841 tgcattaaca ctgccatctt gacctggacg cgagattacc tttgcggtcc cggcttctgt
     9901 ttcaaccaac gcacctttac taaccacccc tctcctctca taatctttgt ttgcaggatt
     9961 ctttgtgaca cgtattatcg ccaatttagt cgtttttttt gaactcggat ccaagacatt
    10021 cacggtttct gcagatttta aggccgtctt tatgttgttt cctctcgtcc tcctggtatt
    10081 tgttatggta gtacccgcga tagcctcgtt tggatatctg tccagttcga atttccgtct
    10141 agctcttgac gcaactcgac gaccgccaga agctttacgt ccagctaggt tttctaatga
    10201 cttccgcatc agtaatcgcc ataagtatct tcctcttatt aagacctttc cgcttcctct
    10261 atcggcgatg ctgttgcggc gatcgttggg ttgttacgat gtgttagttt attattttta
    10321 ctctgattat cgggcgtcgt tactagcgaa tttcttaaag attcaaggtc tgttacccca
    10381 aaataacttc gaaacttctt tgtagttcgg tatgttttcg accttccgag tttttcgagc
    10441 tcgatgaatc ccatttgctt caaggtttta agatgggcat atacttgact tcctctaatc
    10501 tgcatgagac gtttcgaagt cacaggctgt tcatatgtaa tgtacgacag tgtcttaaga
    10561 gccgatgttg gaattaacgg acgctgcgca aattttctga ttaggggtgt atagccaggt
    10621 tttaattgaa gcacgaaagt tccatcagct aattcagtta tctctatggc cgtgaaaacg
    10681 gattttgttt ttttaattaa ttcgttaacg actttccttg tatttgattt tgaattggtt
    10741 ccagatgctc ttaccaattc ttcaattgac aagggccgac ccgccgaata taatgcagct
    10801 tcgattctcg cagtgatttc ctcagctgga agagaaccgc tcaacttcgc atacctattc
    10861 tacttgtgga tttagtaatt cgcatacatg ctcacttgat cgtaattttt atgtcttcag
    10921 aatcctcact ctgttctaga tttatttttc ctttagtcac aagataaagt aatgctataa
    10981 agtacctagc aacctctaat gctcccattt ttgaaataag cattctaaac atcaccacat
    11041 ggtctacatt aacgatacca aaaatcttat cttcatattc tcgtattatt tgttcaaatt
    11101 tctgcgagta ttgatcaaag tcaaaacctt gtactggttc gatagtaagt ttttttctcc
    11161 tcgagtccgg gtttgagatc tcgaagatca tattctctaa cacttgaaga agttcttcta
    11221 aagatacggg gtacgttgat tcgaacctga atggaagctc aatcgcatca agatctgtta
    11281 caggatcatg agttactgga tctatgatcg tcttcttctg catagctact ttctctagcc
    11341 taaaaatgct ttcgacttta agacggtgta tcatcgatga agataaagct gcaattccgc
    11401 ataatcgaag atcttttttt ccggaggcgt gaataatatt tagcaaaagt ccgagaagct
    11461 tacttatatc tatatcccaa acatctttct tgctaactaa agaagggtta aacaataaat
    11521 ttagaggtgg ttcggcgatc ttcttttttg aatcatagtt gatggcaaca tctgagttat
    11581 ctcttccttg taacaattct aaatctaggt acatgaggtc tagtcaggat ataataaagt
    11641 agcaaatcaa taaaagcgac gtacatgtgt gaagaacttt ttcctagctg tgtgtttatc
    11701 ttttggaaga ttgaggatta gcatctaatt cgacagtgtt tgagagcatc tctcaagttc
    11761 gatcagctga tgcttactag aactctaata gtttctttgg ttcagcgaat attcgcataa
    11821 actgttctta atcaattaat taaaacagca agggttttgt aatcgctcgg attgaagcgt
    11881 ctcgatctag attgaagggt tataaaccaa tgagcacttc catcgaagaa acagcggaga
    11941 atatgtcgaa ggttcatgtt caccatatgt gctatttgta gtgcgaatag aataactgaa
    12001 gctgcacatt cgtaggaacg aatatcatgg gatatgtgct agagttgggg tcaaggtagt
    12061 attgacatcc acaaagacga taacgtagag gcatcacagt catgcacaat tttcacagtc
    12121 gtctccaggt aggccgtttt aatttttctg tagccaagtc cagttaattt catccactat
    12181 acatataatt tgatttgtga taagaatcta aatagcatga tttaatttaa attcggtcga
    12241 tatatattat tttgtacttt tggaacagca ggacaataag aacagagttc gagctgaggt
    12301 gaaatttccg ctcttactgg ttatgttctg attgtgcctc gctacctatt gacctcagga
    12361 aatctacgtt atttctggct cttcgtcaga tgtgccaatt cgaatggaca ccttattaat
    12421 caattgatat gatatatgtc agtcaagatg catctcaaac agcatgattt gttttaaata
    12481 accctaatat ccaataatac cgtttggtac aggagaaaga aaagaacaaa attcggctgg
    12541 aatggaagtt tcccgttata ctgatacata cgccatttgg tctgcacttt tttgataggg
    12601 ttgcaaatac caaagctgct agagtctatg ctggatttag catttatctt atgccgctta
    12661 ttactgcatt aggtatattt ctaatagtta cctctctgat agtgatttct tcgaacccgg
    12721 gagcgagagc tggtcaacgc gatctaggtc cacaggctaa tttgcttatc cccggattga
    12781 atccatatct accatggacg tacgggtgga ttgcgttagt gatcactatt ataatccatg
    12841 aggcaggtca tggcatagtc gcccgagttc acaatattaa agtagactct acgggacttc
    12901 ttctaatttt aggacttcct cttggcgcgt ttgttaatat cgcaccagag gagttaggga
    12961 gaagcacact aaaacaaaaa agtgcaattt taacttcggg tccattaaac aatatgatac
    13021 tcgttgcgct gtctttgatt ggactgtact ttattgtctc tactcttact cctattccaa
    13081 ctaacagtgc acctcaatat ggggtagtag ttctgggtgt aggagatcac actcttgcgg
    13141 gatcgatagg attatcgaaa ggatctatta tcgaaacagt agcggggcag aagatccaca
    13201 atttagaaga tttaggaaaa tttctcaggt caaatctggg taaaacagtt aacataacat
    13261 ggaaagacaa ggctggacat cagataattc aattcgttac cctaccaaaa cacgttgaag
    13321 caaaccaggg aatcttgggt atctcaataa ccaatgccgc accagaccca gcgctggtgt
    13381 taagattata caagagtgcc ttcaaccctc tttccaatcc tgttagccat gtggctctac
    13441 tttttcctcc aacaatagga gcgggagtgc agtcagatcc atattcaaat ctaatggctc
    13501 ctaaatatca gtcaagcgtc ctcggatccg attttgctcc agtcgccaat ttgctatatt
    13561 ggataatgtt cgtcaatttt aatgtggcaa tttttaacgc gcttcccata ggaccttttg
    13621 atggtggtca gctttacaaa tctctgattg agaaaagatt agggaaagga gaaggagaag
    13681 aagaaattag ggcaaagcaa gacaaggtca aaaatgtgtc gcgattgatt acgggggtga
    13741 tggtagcaat ggtagcaatc acggtcttct ctccatggct ccttcggcta ctgggactag
    13801 aatgaatatc ttattcatat cttgtggtcc tgtagaaggg ttgtcattgt tgccacttcc
    13861 gctatcgctt gggttattgc cgttgtcaga tgatgggggt tgttgtcgct gctacttcca
    13921 ccgttatcag gtggacaggt tgcagtagca tctatcgttg agccatcagg ataggtttgt
    13981 tgttgtggtt gtgaaggagg acatgtcacg ctgttgatac tctaactata ccgcaattcg
    14041 tagaggcagg tcggggaatc ttgggtatca cgataacaga tgtagcaccg gatccgaccc
    14101 aggtattaag cagatacaag aatgccttta gttccaatcc tatagcacta ttgttgcctc
    14161 ctaccatggc acaaggctca gttccctatt ctgatctgat ggcccatgaa tatcgttcaa
    14221 acgtgttagg gtccgctctt cccttagttg ctaatatgct gttttggtta tggtttatca
    14281 attttaacgt gggaatcttc aacgcactgc caataggtcc gttagatgga ggccaatttt
    14341 ataagtcgct aattgaaaac agaataagat caaggccgag cacattgaag aatgcccaac
    14401 gctggttacg ttagtaatga cagtgatagt ggtaatagct gtgttgtttc catggttgtg
    14461 gccgcattaa ggtgccaaaa caagccaaat atccacaggt cggtaaaatt ttctagatgt
    14521 ggcagctaaa ttttacttag gtacccctat ttcagagttg gatcatactc ataaaaaaag
    14581 cctaccagcg gacttgacag aagataggac gtactctcac aaataaagaa actgaaaaat
    14641 atattcctac attttagact aggaaaacaa gcgcaatcgc ggtagtacct cttattagta
    14701 gttgctcgta tcatttctca ggtatctgca aatgcaaaga atcattgcag gctgatgctt
    14761 cggtatttca tcgtcagtct gcaactctta attaggggtc tgctatagta tcgcaattac
    14821 ggacgggaaa aatataattc caagaacatt gttgtagtta tctagtggta cacaacatac
    14881 gaaaccctcc ccttactaat tcggatccca tgatgcatac ggagcaaata ttttttgagc
    14941 ttgccagcga gcagagactt tcgattcttt tcaaattaac cgagcacgat tctaagcttg
    15001 cgaaactatc taaggatctg aatgtcacta tgcaagaagt ccaccggaat ctcagcagac
    15061 tagtggatgc aggtttgatc gagaaggatt ccgcaggaac atttgcctta acaacgttcg
    15121 gaaacgcgat tataactcaa atttccacct ttggatttct ttcacggaat agaagttact
    15181 tttccgaaca cattttcggc gaaattccta tgaagttcat ccaccggatc ggtgcgttag
    15241 ataactccga atatatcaat ggcctcgtcg ctgttattga attatggaaa cagctataca
    15301 atcggtcgtc tgaatatatt tatggaatgc taccacaaat cccgttagat ctgattgaag
    15361 ccatcatgcc aaaaataagg aatgaaggtg tcaaatttaa ttatatatta ccggaacaag
    15421 ccatcgtacc taagaataga gctgagattc tcaaaaattc tggttatcaa gaattgatga
    15481 ggaaaggtgt ggtagagaga agaatggtgg ataaaataca agtggctgtc gttttaagcg
    15541 aaaatcaggc aacggtaatg tttcctactt tgaaacatga gacagacatg aattctatgt
    15601 tctatagcag tggcttgcag atcgataatg gtatattcca tgaatggtgt cttgatttct
    15661 tcagatataa ttggtataat tc
//
LOCUS       DAOR01000194            3980 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_46520, whole genome shotgun sequence.
ACCESSION   DAOR01000194 DAOR01000000
VERSION     DAOR01000194.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_46520"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2195..2343
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1167 a          725 c          705 g         1234 t
ORIGIN      
        1 atcgaatcag cgttggcgtc tcctttagtc ctgtgatcca tctattaaga aatacattgt
       61 tgtatatgac ttatcataaa ggccattgat ttattcaaca agacagtgac tagacttaat
      121 ctagtgaaag catacattct aagctatgga tatacttcaa ccaattgcat tgctggcaat
      181 aagctttgta gctatatttt gcagcatgga gatactttgg cgactgcatt tagaaaaaca
      241 agcagctgag ttgaaccgga aagaaaggat gcaagaacca caaaaatgtc attatcaaca
      301 gaaatttaaa gaagggtgtt gaggttgctc tcactctatt tgaataaatg atattccaat
      361 aactttatga gcctaccagt ttatcatatt atctggtaat tcaatgactg acgcacctgt
      421 ttcttcttcc tcctcttagt ctacaaataa ttctcaaaga aatgtttcga tagttatgtc
      481 gattcttatc tttacattaa tagttgataa tcttctttat gcctttcttt ttggtggagc
      541 acaagcaaaa gtactaactt cttcattagg tacttcactt ttcatattag ttgtggcggt
      601 ttcatatgca tcagctctat atcttctttc acgttttgtc tctccaatac atagacagat
      661 caagacccat tcttcacgct ttaattggat atacaaaaca ctactaatat ctcaatatgc
      721 gtcagctgga attattgctt ttatgattct tcagctaata ttgacctcac attactacgt
      781 tggattgtta attgcaacca tagcaatcag ctttacatta gccgctatta ttttgggact
      841 gttaagctat agattccttt catggtacaa gtcatcaaac aagaatatta cagtattgtt
      901 atatggtata acttttgcag taactgctat aggtattgga gtaatagttg ctgttaatag
      961 cagcgttatt ttattagaga atccttccca aataggagga ttacagtcag tcttgaaagt
     1021 atactcttca tcttcatcat cggacaccac ccagagtcct aaagcgttaa tactatacca
     1081 gattacctat actccgtcgc gtctgggttt tgtattatat tggattgcga ctgcgttgct
     1141 tttacgtgag tattccagaa gattggggag gctaaaattc tggactctta tcagctttcc
     1201 tttagtatct tttctggcag ccagcatctt tgttagtccg gattacaagc atagcttgct
     1261 ttatgacgcc ttattagtac tatcggccct gactgcaggt ggtgttctat ttggtattac
     1321 gtttttgatt atggcaagaa gtatgagacg catcactacc actagtagta ccaatactgc
     1381 cactcatcac aacaccttag catattatct tactatctct gcatttggca ctgtccttat
     1441 tactgtctcg cttacatcac cagttatata cgcacccttt cctccatttg caaccgctgc
     1501 ttggtctttc ttgggattgt catcctattt gtatagcttg ggtttttatt tttcagctat
     1561 ctccattgct caggatgcaa agttacgaca atctattaga gatattgccg caaaagaatc
     1621 taaactattg gacagcattg gaacagcgca tatggaacag gaaatacaga ataaagttct
     1681 aaagcttgcc aaggagcaag aaaaaactct agaggaacaa actggagtag aactacatga
     1741 atggcaacag caacaagtgg aacaagagaa tatacaagac tatatggctg aggtatttgc
     1801 agaagtagag aaatcacgta agaagcagga agaataattg tagctcatat atcaagtcat
     1861 ctatattgaa tatgatttcc atcttgttaa gtgaaatttt tgcatcattc tggccatctt
     1921 caatcaactt taggatactg gttggcgaaa aatctgtgtt tttaccaaat gtgtcctccg
     1981 tgtcttctac acgatctatc ctccacactt tggttatgtc caatcgtccc tctatgatgt
     2041 ccttatactt ttttgattca gctctgctac gtgaaaagcc ttttgcggct ttgtttagaa
     2101 tctcctcaac ataatcagcc agaccgtttg aattgccaag ttcaattagt tccacagcca
     2161 agtccacgta gtcgcttacc agatatgcta tatgnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnagtccac gtagtcactt accaaatatg cgatatgttc atcgaaattt gtcctatcat
     2401 ggaataatat atcatgagct ctgtcgtcaa taagatcttt atctctggga atattcctca
     2461 ctagtcatgg gtgtaaatta actatttaga cttccaaatc tgggagtcga gctttatcaa
     2521 gcttatttaa acaatctgtc ttgcctgagc aaattattgt tgtcgatgac tcctcttccg
     2581 atcgtacagc ggagattgcc aggcgttttg aaggtgtatc ggttgttcgt agtccaaagg
     2641 ataccggctc aaaaggacat gccctaaatt acgggttaca atatgttaag agcgagtata
     2701 ccatgacaat ggatgcagat ttaacagtag aagataatgc ggttaaaaaa atggtcgaat
     2761 ttatggaaaa ccaaccggaa ctttctgcta gctgtacgtt tcttcttcct aaaaacacta
     2821 acacaatatg ggaacgcact cggttagtag aatacatatt taccctttca ttttacaaaa
     2881 gtgttcagca aatgtatgat ggtattttaa tttgtaacgg atgctttgcg atgtataaaa
     2941 cccaggatct aaaatcggtt ggaggatggc cgacacttac tgttgctgag gatatggaac
     3001 taacttggct tctttacgag aacggaaagc atataggcta tcgcccagag atgggccgac
     3061 tctgaagaaa ttggacaggg tacgccatca ttagttcaag ttgagtcaaa attaggcctt
     3121 gcggagatca ataaatttct ggatgaatta ccagctccaa tctattttat gggcgaacga
     3181 tatcttgtaa aagggaaaca tgacctgcag cagatagttc tagattatat tatcaaaaaa
     3241 tatcctgaaa aaatcaaaga aatccaagac gagatgggag gtcaattcat ctctagaaat
     3301 aaattctata caaagcaaag aggcagcaga aaaatatcaa gtggttacta catggaaatt
     3361 aaatcgccta ggaatagaat gatcaaaaat tgtaatagta tcttaaagtt ggtaggtctg
     3421 ggtagactga cagacgtatt aactataaat tatcaaaaat agcattaacc taatttttga
     3481 ataccgaata atgttacagt catttgctta atgttatata gatttggtaa agaaagtcaa
     3541 agaacaatgt tatatagact tacaaaaaat taaggatggt gacagtggat cacatggggg
     3601 tagagtaaag aaaacagtat cggataacta catgtcaagt acaatccttc atagtatgta
     3661 gcttaatttt ggagagatat tactccggtg gtatgggtta tgatattgtc tcgttcagat
     3721 actcatgaat ttattataca gcattatctt caaacctgct tccactttct gtgcggagaa
     3781 cttctttgaa aaaaggtctt cacctagtac tcttttgatt gaagagaaga ctatttcagc
     3841 aatccatctt ctccctgcat ccttaagctg tttccatcct tgatagccga gtttcttgat
     3901 tagtagtact tcttcccttc ttaaacgaca tcctcctgat cttgtggatg cattatctgt
     3961 ctcttataca catctccgag
//
LOCUS       DAOR01000195            9609 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_28150, whole genome shotgun sequence.
ACCESSION   DAOR01000195 DAOR01000000
VERSION     DAOR01000195.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_28150"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    9146..9358
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3033 a         1675 c         1723 g         2965 t
ORIGIN      
        1 cgtgtttaga tggcatctat gtccacattt caccccttga tcaagtattt ctggacacgc
       61 atcacatctc ccagcttgac attgttcaga tactacctat aacctttttt tatagttctc
      121 cataataaat aactattaac cagaatgaga agaaagcgaa gaaatgagtt aagtaatccc
      181 ttttagaggt cattaatctg gacaaagtag ctttagtgac tggaagctca agtgggatag
      241 gatttgaaac atctgtatta ctagcgagaa atggatttca tacgtattct acagtaagga
      301 atctcgaaaa atcgcaagca ttgattactg tagcaaaaaa ggatggccta ccgcttcaag
      361 tgatagaact tgatgttagt agcgataaat ctgttagaga tggtgttaaa agtgtattag
      421 atgagaataa aagaattgat gtcatagtca acaacgctgg ctacgcctta gtaggtgcga
      481 tcgaggatct atcaatggac gaaataaaag ctcagtttga agctaatttc ttgggagcaa
      541 taagagtaat ccaggcaata ttacctacaa tgagagatca tagaaatgga aggatcgtaa
      601 atgtatcttc tatgggaggt aggatagcca tccctcttga ttcagcttat cacggaacga
      661 agtttgctct agaaggatta tctgaatcat tgcagtatga agtggaaccg tttgggataa
      721 aaatcatcct catagaacct ggtgccataa aatccaactt tttcaataat ttaaagatgg
      781 catcaaagac acaaagaccg gattctcctt atacacaaat gatgcagaaa ttaaatgcag
      841 gattctcgtt catactggaa aacgctcctc cgccggtaga agtggcgaga gtaatactaa
      901 aggcagttac gtctgaacat ccagaactta gatatccaat aggcgatgat gcatcagctc
      961 tacttaaagc aaaaagaacc atgtcagatg cagagtttgg aaatttaatg aagaagcaat
     1021 ttctctctca ataacgacag tccgtaagag cgattgttga ttcaaaagct cactatagaa
     1081 ctgctcacaa attgtagaca gagacggctc gatataggtg ctctgaacgt gagaggccta
     1141 gcgcttgatt catgcgtctc tgagcctgag ttttgtagta tcgcagcaag tcttctttga
     1201 ttctctattg tacgtagttc attaatctta tgccattcaa actacggcgt atgttagcgg
     1261 acacaaactt gtgtagacgc ttgttttatt taagcaaggc gagtatcctt gtcataccgc
     1321 caatatggcg agcggccgcg agaaaaccta atcttgaagc actgtaccaa tatgtatatt
     1381 gagtttgctg tttaacaaaa actcccttat ttgttgttca gttaacggtg atccaaattc
     1441 tggatcagca gtaaggattc gcattattat tgttagcatc cttttcattt catgagggta
     1501 taattgttta cctggatgtg tctttacagt ggagaagcgg tgatctagga aaaaacattt
     1561 cctcgtccaa atatttgtgt gggaagaact agtagaattt attagtcgtt gcatcagaag
     1621 cattagcaaa ttcaattcta gtcttgtaat cgtttacttt atgatcatga caggacattc
     1681 tgcaatctct gaaactcttc ttgccacact tccaagaagc ttcagcttgc cagcaacata
     1741 ttccttttct ctctttagct ctcggctgcc cataactatc aagtctacat gttcgttttc
     1801 agctacatca attattcctt ctgcaggtgc tcccatgcct gttatgaatc tgacatttat
     1861 tccatttgcc cttacttttt ccattctatc ctttagcatt gcctctgcac ccgccttcaa
     1921 aatattaagt gcttgcattt tagcgtcctc gagtcttgat ttctcgccaa tgaaccccag
     1981 aatggcactt gatggtatta atctgtcatc tactatatgt aaaataatta tttctgattt
     2041 gaaagcctta gaaaaatcta tggctttgtc caaagcctta tctgacattt ccgaaccatc
     2101 atgcggcact agaattcttg acgttgttgt tttgctcacg aattttaata atattaaacg
     2161 acatatattt ttactttatt gctatattaa atgacaaact atgatgcccc ccagtatctt
     2221 tttgtctaat tgaaggtcaa ccgacatgtg ataaggaaaa caatattgaa ttacaatttg
     2281 agataccaga taatagatac ataggcattt tgattatact tgaagaggaa aaaagcaaaa
     2341 ctttcattct aaatttttta ttttcataac ttttaaaata atcgagcaag ccaagctgtt
     2401 aaatgtgctt tatgtcactt tactaccaat acaggacaat gtgcataggt tacaacccca
     2461 gacgccacac ttccaagcag gagcctttta aaacctgatc ttcccttcgg aagaaccgaa
     2521 ataggacttg gttattaaaa ccagaggcta atgacgttaa gttatgatct aaacaaggaa
     2581 acaaacaagt actgagtata tgcattagta ttatggccaa tgttatcaac aatacagact
     2641 tggataaagt agcccgaact gttgcaaatg gcaagaaaga taagcaaagt ctaagaaaac
     2701 cagtcaagct gcaaggagaa tggaatctgg atccaagcaa ggcatatcaa tttagaacgg
     2761 aaatatcata cgagaaggga aatgaggtaa ttgaaataga ttctccttca tttttaggtg
     2821 gaaatggtaa tagattaggt ccaatggcat attgcgtagc tggtataact tcatgtttca
     2881 tagctacatt cgcaagtgtt gcagcaagtc agggagtaaa gctcactaag cttattgtca
     2941 atacggaatg catgattaac tttgccaaga cttttgatat agctgatgaa ccaataactg
     3001 aaggaattaa ctttgatata gctgctgaaa gtgatattgc agataagcag aaactataag
     3061 aactggttaa aatggcagaa gaaagatgtc ctgctatgta tagtatgtct catgtgataa
     3121 aggttaacgc tcagataaag taataggcgt agatatgcac ttgtgaagag agagtgagct
     3181 tgtcacgcca gcgtaagaga ataacagcga tcaagtcgat cttttgaaag taatgctgaa
     3241 gttgatgcaa ccaataaata caagattcta gaaatatgcg atatggcacc ctcttcggga
     3301 ggcttacaaa cattcgaatt taccagataa agaagatggc tatgtatcta tcggatagta
     3361 tctatacttt atattgacgt gatttggttt tttctatcag tttatttaat gatggcggtt
     3421 tttttattcg ataggcattt gaatgttgga taaagctctt taatattttg aatcataatg
     3481 ataacccacg ggacgtaaaa aaagatacaa acaccaaaaa atctagatat tgtggttgcc
     3541 gaaccagata aggatatgca ccaactctac aacctttatc ttaaaaattt agggataaga
     3601 gaagacaata ttatcgtagc agaaagtgga aaaaagtata tagaatcgtt gttagtaatg
     3661 ttaatgctgt tgttgctgaa gtagaagtca ctaggattac caaggctaag tataggattt
     3721 gatcatcttg atcctgttct ataatctcgt tgttctatac catcaaaagg aatacttaag
     3781 ggagaattat ccacagacag atcgaaagta gcaacattgt ttttccttga taaattgggt
     3841 cgccgtttat tctagcctca aaagtcaccc tttatcatgg atactaaaat tcaggcatac
     3901 gcgaactcgt aatattaaca gtgaactctc cgttctatct tttcaatggg gtagggtttt
     3961 atgatgcagt cagaacttct aaacgttgta acgagtgtat ataactaatg aaattactcg
     4021 tagctggaaa tgatggataa tctctatgtt gcattaaatg gcaatttatt attgtactat
     4081 acgactactt aacaataaat tcagcaaata attcctaacc tagtagcgat gtttgggatg
     4141 tatgaattca tgagtatctg aacgagacaa tatcatgacc aataccacag gagtagtcta
     4201 ttgttattac tttcttctac tcttttcctt ttgagtagtt ttttgaattt gttttatatg
     4261 tctttccagc tgcttaactg aagtggctta aatgattctg tttgtttagc gaaatcaatg
     4321 aaattttgta atctttaacg gttcggctat ctcggtcact acatttcttg aaacatggtg
     4381 acaacaaaaa aacaattcta gtagcaaaaa aattcttatt ctactttgga cttcatttta
     4441 ttgcaaaact tgtgcttgca gcgactggta gtgtgctgct tgaattaccg ctagctctta
     4501 aagatgtttg agcattgtaa gtccctgctt gtacttgttt tccgtcaata tctttttgat
     4561 cccattgaat cgtttttgat tgctttggct ttagctcaga cattacttgt gatccaagca
     4621 atccagcctt ttgctgagtc ttggtatttt gtattgttaa acctagaagt gaatcaggga
     4681 attctaatgg atatttccca atgttcttta tagtaataac aactgcctcc cctggtttgt
     4741 aggtttgctt gctgctggta atttgcaaat tgggattatt attactatta tcataatgat
     4801 tataaacagt tgttgttgcc gtcgttgttt tatctcgagc agcagtacca gtagtagtat
     4861 cagtttgtgc atatgtctga tgtgagtagt taaaaataat actaatagac agtacaagaa
     4921 ccggcagcgc aatggtcaat aggcccatca ttttaacatc tatttccaca gtttcatcat
     4981 ctcacaataa aatatattgg taggatactt tgcctcccct ttccccgtaa ggtgtcatct
     5041 tgatactctg tcagcttgct gatttgtgct tactgaatta actgaaacct gttctttccg
     5101 tgttgtttat ttggcttacc tattattgaa ataggatagc cacagttctt acagcaatta
     5161 ttggcatcta ggttccatga ctctatacta aatccatatc gttgcactgc aatatattta
     5221 catgctggac aatatgtgtg ttctagtgga tggcctggaa cattcccaat ataggcataa
     5281 cgcaatccta cttcctttgc aataccatga tgtttttcaa gtatctcaat tggagtcata
     5341 ccaaattcca tcatcttgta atccggatgg aatcgcaaaa agtgaattgg agtgtcaggt
     5401 cccagttcat catagacaaa tttgcacagt tttctagcag cttctaaatc gtcaccaact
     5461 tcagggacta tgagatcagt aatttcagta tgaatacttg tcttgtctct aatatccttc
     5521 agagattgaa agattgggtc agcattagga attccaatat atcgtctaac gaattcttgc
     5581 ttgccacttc ccttgaaatc gacggtaata caatccaaga acttactcat catgctgact
     5641 gattcctgtg ttacgtatcc atttgagaca aagatattaa aaatgccttt cttatgagcc
     5701 tcaatcccac aatctcttgc aaattcgata aaaatagaag gctcattata tgtgtacgca
     5761 attccatgag caccataact tatggcacta tctacaacct cttttggtgt catgtctacg
     5821 ccttgtattt tacgtctctg ggagatatca taattctgac aatatttaca aagccagtta
     5881 cagcctgtag ttgctattga gaagatatca gtaccaggcc tataatgtgt tactggcttt
     5941 ttctcaattg ggtctatgtg gcctgcaata accttaccat atacaaataa ttgtaatctg
     6001 ttattcacta ctcctctaat cccgcaaaga ccagttttac cttctggtat ctcacaaagc
     6061 ctagcacagg cagtacattt tatccttcca ttaggaagtt tatcgtataa ttctgcttct
     6121 ttacctaaga cttcgtttcg ttgttgcatt ctatttagat cttaaccgta tgatctataa
     6181 aatatgttag aggccactgc attgcactgc agccatagat aatatattac caatatggta
     6241 cctgactatt aaggcaaatg tatagactcc ctttatcgga aaaaattggc gatctcgcag
     6301 ataaggattt tgtaacgtta gatgaggata cattggttgg tgtagcagcc aagacaatgc
     6361 gtgataaaga tgtatcatgc gttttagtca ctcgaaagga ttcaaactca aatgacccaa
     6421 taggtattgt aactgagaga gacatgctgt atcgagtgtt ggctgaaaac aagggaccat
     6481 ctaaagttaa tttaggaaga ataatgagct ctccacttat tacaattggt gcaaactttt
     6541 cagctgcaga tgcgatttca ataatgagaa ataaaaacat cagaaggcta gccatacaga
     6601 aaatgaaagg aggaaaaatt actggaatag ccactcttat gtctgtagtt ggaaatattc
     6661 caagccaaag catagaatta gcagaagtcc aattacctaa agatcttatt ggaggaggaa
     6721 gaggaattat tagctgtctt tattgtaggt cgacatttga aagcagggat aaaataaaaa
     6781 gacatataga tgaaactcat cttttagaat cttgactatg aggttcttat tcactacata
     6841 gcatatagaa atttgaaaaa atccaacgat aaattaagaa atatctatta atagcaactc
     6901 ctaaatgcca cgtttccgcc aataacaaca agttgccaga tgaatttgct gccgatatca
     6961 gaaaatcaag tcacgaagaa gaaaatttag gtgaacataa aacaagccga agcattactt
     7021 ttagactaga ttctcataca atcgacgaac tacagcgtga agcagatcaa aatcagatat
     7081 cactgaatgt tttgataaac caggtactga aaagatattc tgaatgggac agttacgaaa
     7141 atagaattgg catgatgcct gtacccaaag caatgctaac ttctttaatt gataaagcaa
     7201 ttgatatggc taagaagaat gggataaaag aagaagatat tggacctgtc agagaccaaa
     7261 taataagaca agctgctgag attgcattta atataatgaa ggatgctgtg ttgtttatga
     7321 agaagcaata taatctttgg acagtactat cggtattaca agagtacatg aaagtatcag
     7381 gcataaactc agaccatagg gtagaaggtg gaagcaagca tgtatttgta atacagcatg
     7441 aactaggtga gaattggtct ctctttacaa aagagctgtt aaaattgata tttgagaacc
     7501 tagctaaagt tagagctgaa gttaatgtta ccccaaatat gactgtggct gaagttattc
     7561 tttaattgca gaacaatttt gataattatc tagaaaaatg ttagggtaac acttacgaaa
     7621 atcataaaat tcttagtgca gtgcatacat actagttaag tagcgtcacg ttgtttgcct
     7681 tacttaaggt ctattacaac aatgatggct catgattctg gtcataataa aaggagacga
     7741 gtaaaaccac ttactgacca gaatatagca cacgaatact ttagactcat taaagctaaa
     7801 gatatgtata gattactcga tctttttaca aatgatgccg taatcaatga accatttagc
     7861 aaatcagaag atggtaaagg aggattacaa ggaaagtgca atagagcctt ttcttaacgt
     7921 cgcaatgatg gccactgctg ggttgagaga cgaaatcgag atcgaaaaat caaaaccgag
     7981 aagggataat ggcatcaatc aagttactgc atatgtgact tttgagagag gaggaaaagt
     8041 aaaagcgaga ttcacatttg tattaagttc agaagggaat tacaattttc aaaggcagaa
     8101 aaagattcaa tctttgcata ttcagtttgt taagtaaata tataatcata agagaaaaag
     8161 gctattaata aaatcaaatt agatacggtt gttcttgaaa tatcactatt acatgttttt
     8221 ctctactttt agtatttttt ctataacttc agtaactgct tcagctggtc tatcgccgac
     8281 actctggatt atctaatgat ttcactgaat aaggaatgtc aaaacgactg gagaaaacat
     8341 ttatcctacc ttacaacaag aacagggcaa tgggcatgac gtaccacgcc atcagctacg
     8401 cttcctatta agaacctttt caatcctgtt ttgcctttag tacccatgac tattagatca
     8461 gtattattat ttgcagcata atttataata gcatcagcga tggatgaaac atctgcaaca
     8521 atatccgtag ttacatcatc ttcactcaca gtcatccttt tacatgtttc aattattttt
     8581 tcaaaccatg gtttagcaga ttttcctagt tcgtcgtaat aagcctgaag agaataaaac
     8641 ggttttcctt cgaattctga aatattttca ggcgtcggta tcacgtgtat acaaattatc
     8701 tgtgcttttt cacacttagc tgccttgatt gcatacttgg cagcctttaa tgatgcatcg
     8761 gagccatcaa ttggcacgag tattttcttt atatttatct cttcttctga catattcaat
     8821 atatcactag actccaataa ttatatcatt atgagaaatc aagttaacac tccgacttaa
     8881 tcgagttacg taataaattg gaaagtgaaa aagtgtggat atgttataga atttatacat
     8941 agtttgaaac ccacttgcta catatttttt cttttacttt attactaata cagggcaatg
     9001 tgcataagtt acaattccag atgcaacact cccaagcaat aatttcttaa agcctgatag
     9061 tcctcttgag cctattacta ttaaatcaac accctcatgt tcagcataat ctactactgc
     9121 tcctactatt gatctagaat taacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
     9361 ttgattgaca gatccaccga atgaataagt agaatataca tgtttaattt ctgaatgaat
     9421 aacatgtaac gcgatcaatt cagcattatt attctctcct ttttttctag ctattgcaat
     9481 tgcataatct gctgctttca tagccggctc tgagccatct gttggaagta atattctgga
     9541 aaattctttg ttctttgctt tgtacatata agaatctcat ccagtgatgg agatttaaaa
     9601 agtttgaat
//
LOCUS       DAOR01000196            4761 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_66048, whole genome shotgun sequence.
ACCESSION   DAOR01000196 DAOR01000000
VERSION     DAOR01000196.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_66048"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1515..1680
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1395 a          936 c          816 g         1448 t
ORIGIN      
        1 ggtttgtacc tatagatgaa tttgagatta ggcgtaatac tatgtttcgt cgaatagtat
       61 aacaaaaaca tgagaagctg ttaaaaccca acaaagtttt tcgtaatgta agccttcctg
      121 tacctccaat tgacccgctt gttgttatcg cgttgttaca actacgataa caactcctaa
      181 tagtaattaa aaatggagta agtttgatgg ccctgaactg gacagcacgc gtataacctt
      241 gcatactgtc tatattatga ttgaccataa ataaagtaac agcattcgaa gagatttaca
      301 gagatgtttc ttctaataat aaaagtaact tcaatggatg attgccctcc atccaaatat
      361 atatgttgga tttcgaactg aaccaggtaa tactaaattt caaattcgat tacgtgttac
      421 tactgctggt agcaaaagtt caatgcctaa taaatcaggg tagtaatagt tataataatt
      481 atagtagcag ggggagatct tattcattat tataatatga gatgccggct cacatgaata
      541 caaaatattt tatttgttcc atggagaata tgcaagaaaa tatatgattt ttagtatcat
      601 tcgcccatgg tctggttttc gataataata tcttccaatt ccaaggtact ttttggcaag
      661 cctttttaga tgctcatctg cgccttcggc cgtctgctca attccccttc cttctgtaga
      721 caccatattg tactgattgt cctgctctac aacagatatt gtacggattg tcctgctcta
      781 caatagatag ggctatcctt ttatcatttg ttatgtgctt attttttgct gatatcgaga
      841 ttaccgccac atgcacaaaa aagagatgcc gttctgagtt gttgtattat atccgcaccc
      901 agaagccatt tctaatagct gaatgccaga ttccacggca cttacatttc aatatacata
      961 tttagttact ttatgtatta cctctgactc gtcataggag gcaagctaag gtcatatgcc
     1021 actaaactta tccgcataca accaattttt atgtgtatca aatttcacca ccataaacag
     1081 cttgtcaaac tatctgaaga aattgagtca acaactatct gatgattggt tcaaacattg
     1141 ttggatcgac tcttattctt gtctatgatg ttttctagta tgaatttgtg ccaagaccca
     1201 attattgttg ctataataat agaaacgagt aaggaataga ctcgagtata ttaccaagat
     1261 tattcaccaa atttgcatca aaatctttta aaggaacagg cttaggtttg ttcatatcga
     1321 agggtatcat agaggattac ggtggtaaaa tctggggcga taataaccct gatggcagag
     1381 gagccacatt tccattcatc ctgtctcttg aggaaaacct ttgatctctt tactatcggt
     1441 gcattttcat tatttctaag cgcatctcca agacccattc tttgtcactt ctcgtttatt
     1501 aaataaatat acctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 tttaaataaa ttcagttcca taatgtcttt caataacgaa tgacggatag cacatgccga
     1741 aggctgatta ttcaaaatat catataatag tcctaaagca taattgctaa actcaaacaa
     1801 cataattgtt gatcatcaac atataatttc tccatttgtg tcacgaacaa tatgtaaaaa
     1861 tagaataacc taattcattt cctctcttca agcacagaca acacattgcc agcggggtct
     1921 ttaaaccacg cgatcttcgg accctcgcca ctgagagaaa cacccttttc gtctgtctta
     1981 agattgtctt cattataaat ctcgaaacgc acgccacgtt tggtaaggtc gtccatagcc
     2041 gactctagat tgtctacagg aaaattaagg atggtaaacg ctgctggagt atggttagcc
     2101 ttaggatata tccagatttt cgtcccgcca tcgatatgaa gctctaacag cctcccgccg
     2161 tacgattcgg agacttctag cccgatcgtc tggccataga actcctttgc tctttgcacg
     2221 tcgtctaccg aaaaactgga aaacgctttc gtgtctttga acattgattc attccctact
     2281 ttcgtatgat tggtaagtta ctcacttggc gctctgatta gactcccata tagcgaaact
     2341 gttgttttct gtatcaagca aagtgcgaag taacccattc ctggtactgc atgtttagga
     2401 gcaactactt tgcctcctag cttctccact tttgacgagt attcgtctac tgattttacg
     2461 tctataaaat tagttatttg atgctctggc atttgtcttt tcatcattcc gccacctaac
     2521 gctttgtttc ctttatcatc tgtggttgtc actatccaat attccattgg ctgacctgct
     2581 gctgaggtca gctgactatc atctgtacca ggccattttt ctattttcca tccaaacaga
     2641 tcggtataga atttcttggc tctttctgaa tcatctgttg gaatttcaaa atgtactatt
     2701 gttggcaaat ttctttcact actcggaata actaggtaat aaagctcata cattatagag
     2761 atgttaaatt taaatttaat ctagcttgga tatatcctcg tttgagaatg gaaagcagcc
     2821 cattcaaacc agaggccttc atcaaacggt aagcgtgtaa gctgttttcc ttttatgtat
     2881 cccttcatta ctatgccatc aaaacttcat tcgcatccaa ccaaatgagt caatgaacaa
     2941 gcctttcagg atagctcgga ctgcattatc gcatgttgat tatgtctcaa ttaatcttca
     3001 atgtttgcta tcattaaata ttctgaattt ttgagtaaga aggagctcca gccacgttat
     3061 acattcatcg gtattcatac tgatgcacct atgtttttta actcttgtag tgctctatcg
     3121 tagagaacag atgcagcatc tttagctaaa acaattctgt aatctcgctc acttgcttcg
     3181 taaatggtta ttctaggact gcctctctat agccctgaac taaacgcttg atatacgtta
     3241 cagatgttaa cgtgccctaa tcggctacag caccgattag actaaaatca cgtcggacat
     3301 caatgataag cagagcagaa tgtttcctgt cgggagtaat gtattcatcg cccatgatgt
     3361 tttttaacag atgtaaataa tatgaagata taacatcgta tgtggtaaaa gagcataaga
     3421 ataatgatgt aaaaaaatac agatctggct tcacaattcc caataactcg gtattgacaa
     3481 gtattaccat gatttggaac aatgagcact agtgaaaact tacctcccct aggttgtggt
     3541 caagcgatct cctgtactaa ttgtcgaaca cgtcatgtca taaatccacc agcgtcagaa
     3601 tatatgtcta ctctgcttga tccttgtccg agagatgatt atcaggggta gaggagctag
     3661 cctaatcaac atattattca aacgaagata agaagagact agtaatctag caacccggca
     3721 agaaatccat tctcatcact ataaaaagga cttctacatg ttgcaagcag tcttcgtttt
     3781 tttgggaata ggagcccttg ttctgttgtt accaaccata tgtctatggt caacatacag
     3841 cgttttccag ataatatggg aatattttct taggtaaggt aactagcctt ggcagagctc
     3901 tgacacatct tgattctctt gaaaggtata ttgtactatg ctcttacaaa gctttgacgg
     3961 gattataaga aaaaaagctg gtttctacta agccccactg aagatactct ggactttgtt
     4021 ttaattgtac agtaatgatc tggatcgcct gagattcacc gttggccttg gctacatttg
     4081 ctcttgctgc tgcttgagct tgtacaactg tttggtttgc aattacttgt atagccaata
     4141 gattattttg ttcagtcaga aacttttgaa atgctactac ctttgatttt acttgactag
     4201 caaatgctgg tgggtcacag tatactgtag ctcggctaat cattacggat tcacaaatct
     4261 ccctatcggt cattctttac ttcaaccgcg tcatacgaat ttgagccatc aagtatacgt
     4321 ttacataaat gatttataat gactaaatgc atataatgaa tatgactaac gagaatagtc
     4381 gtccatacat aaccagaaat gtttatccaa ataaaagagt tccagcacaa atgaaccctg
     4441 aacaaattgt acagtcaata aagaacattg ccaaaggaat tcacgaagga tcatataaaa
     4501 tgacaaaaac agtacgaatt cttcatcaaa gtggagcaat taacgatcta acacatgcgg
     4561 tatatgatgc tacagttgct gcacgtgaca ctgcaagaga aattcgtgat actgctaggg
     4621 atttgaaaga ggacggtata atcggcggta cagctaaagc catacaagaa acaaccgtgg
     4681 cagcgcgtgt gacagtacac acagtgagag atataacaac cgagacaacg cagacactcc
     4741 ctaaggcgat catgacaaag c
//
LOCUS       DAOR01000197            5756 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_32947, whole genome shotgun sequence.
ACCESSION   DAOR01000197 DAOR01000000
VERSION     DAOR01000197.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_32947"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4196..4392
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1713 a         1070 c          998 g         1778 t
ORIGIN      
        1 atcctcctgg atgaggccag attctatagc cgagtccttt aactcccgtt taggtacacc
       61 cctcttccca tcgctttttc gatttatcaa atctagacac cttatcaatt tatcactagg
      121 gatttcaatc ttatattctg gcagaccaat gatctgttta agaccttcag tttcttgttt
      181 ctcctccttt gccaatgagc tggtatacct ttcagggact acatagtagg gtttaatcat
      241 ggcaatgtct ttgaacatca tacatgccat catggaagca atagcctgaa tcttacttcc
      301 cactgacaca tttacgagaa tcgagttgcc tttctctttc aaaattatta ttctcaacgc
      361 tcgtagtata tcaaaaagtt caactctatc agcttccgtt tgcagacatt ctattcttgc
      421 ctgctttaac ttatctcgta ttgatttgac aaattgatgt ccctcgtcaa cactaggatt
      481 attgtgtacg ataagccaga ctctgtccgc tttcatctcg atggcgggta agactatccg
      541 atctacttca aaaccaacta gagctacatg aactctaagt gtttgaagtg aactcattat
      601 tttcggtttg ctcaataaag tattcatagt aacttaagtc aggtgatatg tattatatag
      661 cttgtgcttg gtataaaaga cagagatcga gacgagctac attaacacaa ggcgtaatag
      721 cattacatta acacaaggcg taatagcatt agaaaaatcg ctaaccctag cagctgacat
      781 aactgcagat aatcagctac tagaatcggt agattgtccc gactgttctg acacgatgct
      841 taagttctat gactgggaca aaatgaaata cctatgcgaa aattgtggat taacaatcga
      901 taatccgcct agttagcgaa ataacaatta cgaacccggt tacaagatgt gaaggcagaa
      961 tagatgcaat atttgagtat ccggaatatg ccgaaacggt agagtggaag acctacgctg
     1021 atggagtctt agcgtatgac cgcaatcaga cgataagtag tgggatgcgg gtgaattaca
     1081 gatctggtag agccgaggat gactttacag gaaatatatt aacagttatt acacccacca
     1141 aggcacacga tcccagagct acaacacttg ccttagacgc tattaaagaa gcacggactt
     1201 acatgctgcg agtactcgat ggagagattg agagcaaagc taccacacag atttgtttgt
     1261 gaatcatgta gttaccagca tgtagattct acgtgggtga caaagtcgac agcgacttca
     1321 agagaatgtt atggagaaga aggtttagga tattgaagaa agggcgagga cacacgttaa
     1381 caaatttctt gcacagcggc tatcacagga gcaatgaaca gaattgcggt tagtccatgc
     1441 tgtgacctct tactagaatt ttatctaata ctgctaaatt acagctacca caaatgccac
     1501 cggactgagg atctgttatg atcgtctgag ctttattaca tacagagcat gttattgatc
     1561 tttgcattaa gtgagaactc actttttgtg cctgaactcc agtgtaactc cacgtattgc
     1621 attataacaa aatgtgtata tttccttgtc agatattggc atcgattcta cttcaccatt
     1681 catacaggaa ggacatacct caactgtgcc acaaaagttt aggtatgatg cacaccaaaa
     1741 acatgaactg cataataaga aatgtggttg cttaaaagtc aatttcatct cgttcctttc
     1801 ttcagcctta atgctggtgt tggttatgac atggcaacaa ctcgcttccg tttttccgaa
     1861 tgtttgtttc ataatcatat aagcattcgg tcattaatac agttttaata gttagttacg
     1921 tatcagagta ttgaatattg ttaccattta ttggtaatag tatatattga tttcttgatt
     1981 attaatttcg tttacctgga ctattagtgc cagccaaagt agggactgtg attttgagtg
     2041 ttgccgaata tcttgtactt ctagatttat tgtatgctgt ctactatcaa atctgctgac
     2101 gctccttttg tatgattatc aattaaggtg gtctttaaag tgagttcaaa ctgctgcagc
     2161 agttccttta tcctcctact tcattttcag tcgcaattca tgagttgcat taacgtttcc
     2221 cgaaacatta tctacctagt ttcaaacaaa aaatgacgct tttgaataga atgcctagaa
     2281 cctcacaagg caatatcaaa agctgtcgaa tggtgagcta acaccgcagc tcaaacagga
     2341 gttaatcaga ctcaaaaagt cggactgaaa cattttttat tacgttagac agactatact
     2401 tgcacaggag aacggagaca gcctgttcca aactcattgt tatgggaaat tatctttacg
     2461 atgttaaacc aaaatttcat gtgggggatc aaaagttatg ccgcctgggc ctaagcccca
     2521 catctcatct catgcaggaa ttgtacttag cgtgtagtca cctttaccat gagtgttgat
     2581 agctatacat ccatactgtc agctaaggcc atttagtcct gaataaggga gtgtaaagtt
     2641 atagagggta caggagtcgg caaactaatt gtaaatgcgg ctccattacc atcaggatta
     2701 ttttcacccc atatcctgcc gccatgagct tccacaatat ttttacagat atacgaccct
     2761 agaacagtac ctttccgtga tgtggtagca aattttgtaa acaatctagg taataccttt
     2821 cgagttattc cagttccagt atcttttata ctaacaatga cttctttatt gttattatct
     2881 ttcttctctt tagttctaat agtgataatt ccctgttttg taaattcaac tgcgttgtct
     2941 agaaggttag aaataacttg agtaatcctc tgtttgtctg caattactac tgttaaatca
     3001 tccattccct gttctggatt tagcatgaat cttatatttt ctctttgttc tttttcaagc
     3061 tgatttattt agtatctgta atagcatgca gtattacttc atttagacaa aactgttctt
     3121 tgtggagtgt taacgcctga ttttcgattc ttgtaatatc taaaatatct tctgtgagtt
     3181 gctttagtct tgtagcattt ctaataatga catccatgat atcatcgtca ttatcatcct
     3241 tttcgccggt aatttcttct tccttctccg ctcttatttt agacctaaga atttcagtca
     3301 aaccaagaat tggttgaaca ggatttcgta actcgtgagc tgctatattt atgaattcct
     3361 gttgcatctt cccatcaatc tttaactttt catgagcctc ctctagctgc ttgttcgttt
     3421 caatattggc tgaagcacag gtatatgact tattatttga ccatatacca aactctgttt
     3481 tactagctgt tttaaaaaat ttaacaaatg ttttaggttg atgcctacca ttgattatac
     3541 aagaatgttg aagcttttgg tgatattatc tgtatgctta acaacttcat ttttgtcagc
     3601 ttcatatgca tacgcaaagg taattggtaa tccactagtt tatcatctta gcagcgatga
     3661 accctggaga gcaactgtag ccatctccga tgctactacc cagttaaaat taggacataa
     3721 tgtaacatta ttgctcagtg tagaaggagt acaaatagga gttaaacatt ctcatcatta
     3781 tttgggactt gatttgctaa ctagaaatgt aacaaatttt attgctgatg gtggaaaagt
     3841 gattatttgt gaagtatgtc taagaatagc tggatacaat aatagcgata tacttgacgg
     3901 agcagtcata ggcagaccgg agatactatc taacttactg accaatgcaa cagttataga
     3961 ctattgacgc agaaggattt ccgcctcttt gttacttgga aaaggagaga tgatggtcgg
     4021 cattggagat cgtagcataa tgagcggttc aaacttataa caaatttgat gaacacaccc
     4081 tttattaatg aattagataa aataagttcg cgacataaac atttgaacaa cttttattgg
     4141 cagacttgtg gtggtacttt tagaagcaaa ataatctgaa tccgacgtca acttannnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nngtgtataa gagacagctt aagggggtta catttttgcg ctaaaattta
     4441 aaaataaaaa aagggccgtg gtaggataac tagaattaaa gggcgatata atattacata
     4501 acttacatga ctaagcacaa tcaaatataa aaataatccg gcaagtaata ttagtaaccc
     4561 ggtcgatctc ctttttggat acatatgacc catactcaga aatccaaaaa ggactactat
     4621 cattaatgac tctaaaccta aactgtaaga actttcggtt aatcgaagaa aaagtcttaa
     4681 actgatcaca ggcgttgaca ttgacatgcc aaacatcgca acactatctc attctcagaa
     4741 cggctcaata tcctgaattt ttttgagcca caattttcac acatgggact gcttcttgct
     4801 ttcactttga ctatctgata tttagcctct aataaaatag gtgcaataat cattaactgg
     4861 tataattctc atattttcac cgtaatcaac tttcaggctg gctgaaatac gagggaggtt
     4921 tgtgttatta tctccgaaaa agaaatttaa aaaaataaag gttcgaaagc ccttaaatgg
     4981 gagtcatatg gtgctccagc aaaaatatta taaaaataag caaacacagg caatatctta
     5041 aacaactaat tgagaacaaa gccgacgtca tgagaattgc gtttcatcct tattttttgt
     5101 ttttcgttac tttttttatg gctcctatga gtcgactgta tcatttcccc atatattgtg
     5161 gttaataatg gtataatttg caccaatact ccttttatca tatcgacgta ctgcaaagcc
     5221 ttcttctaat agataatgat aagcctacag tctacgaagg tctggaatat gtttcatgga
     5281 tattataata ttggcaggaa gtatcacatc ccagaaatat tgagctgcgt ggaggttaat
     5341 aaacgaaatt ccaattgaag taaggaagat taatggaggt acaaacgtcc tcacaataaa
     5401 atacctaatc aactggttgc ccatatcttg aactaccaac ttttgattgt gtgtagcata
     5461 ccaccatatc aaacataaga caaagccgct tgcagctata ataccgctaa aggatattat
     5521 aactattcta tgagtcttat acgtagtaag aagcccagta aaatacccaa ctaaagcgat
     5581 agacagttac caactttttt ttgtagcaaa tctgtgtatt cagtttttat gagaagattt
     5641 cacggattca ctcttttaat aggttctctg atacccatca aacggatagt ttgttcaaat
     5701 tgatcttttc ctcctgaagc gattacctgc aaccgaccta ttccgctcct cctaag
//
LOCUS       DAOR01000198            4632 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_133865, whole genome shotgun sequence.
ACCESSION   DAOR01000198 DAOR01000000
VERSION     DAOR01000198.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_133865"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1401 a          866 c         1002 g         1363 t
ORIGIN      
        1 cagctatacc gcttatttta aaaggtctag atgtcatagg tcaggctcat acaggaactg
       61 gaaagactgc ggctttttcc ttaccgattt taaccaaaat taagcgtaat ggtccgatac
      121 aagcccttat acttgtaccg acacgagagc tagcagtcca agtgacaaat gagatcagta
      181 aatttgtacg atacacaggt cttagaagcg tttccatata tggaggacaa agtattggtc
      241 ttcagcatga tctattaaga aggggagtcc aaattgtagt tgcgacgcca ggaagactca
      301 ttgaccattt aaaacgagga tctatccagc tggatgacgt aagatttgta gttcttgatg
      361 aagcagatag gatgttggac atgggcttcg ttgaagatat aaagttcatt ttgttttatg
      421 tgaccgaaga taggcagacg tgtcttttct ctgcaaccat gccacaggag attttaagat
      481 tggcacaaga ctatatgaaa gaacctaagg agatcagatt aaacgaagag gaacttagcc
      541 ttgatactat agatcagtct tatttgattg ttcatgagag ggagaaattt aagcacctct
      601 gtaacttcat aaagaataga gacaagaagc agactatagt ttttgccgcc acaaagcaaa
      661 ggacgcagag acttgcagac gatttgaagc aggaaggatt cagagcaata acgattcatg
      721 gcgatctttc gcaaagacag agagatatcg caatgtccag gtttaagaat gggaatgaag
      781 acattctggt tgctaccgac atcgcagcaa gaggaataga tgtcccagcc gtaggtcatg
      841 taattaacta tgacattccc gaagatccct tgatttattt ccacagaatt ggaagaactg
      901 ccagggcagg tggaactggt aaggctatct cattagtttc ccaggataga gttgacaatt
      961 tcggaaggat tctccgacag acagaacagc ctgtccataa acttaatgaa gaaatgggaa
     1021 ttgaagttcc tgtcgttcag cagcagcagc gccgatatta cggcaatggc tataatagca
     1081 actacgcagg taaaggtggc tcgtatgggt acaataaagg ttatggcttt aatagaaccc
     1141 aatacggtcg tcaagttgag cagagagata ccaattatag caataggtac agtatgcgac
     1201 gggataggta ttcttcatat tcccagagac aagataacag ataattggat agaaagaggt
     1261 agggtatttc gactcttacc actactaccg tcgaaaaatt caattgctct aatattacga
     1321 cgtttataca gctaatgtcg tctctgtgaa tttttgagtg tacgaactaa aacctattga
     1381 atcttctttt gatcgctaat cgtccatgac gtttgattaa ggaccaaata aaattgtcga
     1441 accgccatta gcgataagcg gttctgccat catcgcaatt gcattcttta tagcctatgg
     1501 atattccacc attccgttcg tttcgcccat tagcagccgt tacctttacc tgcctgttcc
     1561 agttacattc acactgttaa attttgttaa attagtaggg gttgctatac aagatatttt
     1621 ggaagttatt ttattttgtc cttgttttaa tgcagcatat ttagaagtaa tagtgtattt
     1681 ccacgtagaa taatcatttt ttcctccatg tcctgtcgct actgcatttt gatatggttt
     1741 tataccattt agaagtactg aaattgtaca accagtatgc gtcttatttg ctactggtgg
     1801 tgatgatatt ccagatacag ttacattgct gctaaccgga acctgttgac ctttatgggg
     1861 agcagtaatt tttagagatg gtagattggt tgcttttgct actggtgaag aaatagcata
     1921 cgcggagccg gtagcccacc aggagctgaa aataagtcct cctattaatg tacagaagag
     1981 aactatctgt ttgaagtgat ccacattcct tgttctagag tatttttcca tttcttgtat
     2041 tgtatgacat tgcctataaa cactatcgtc tctggctttg ttggttgttg aaaaaaactg
     2101 cacacatgta gagttgtgcc gctaattcag ctattgcacg cataattatt ccgtctcggt
     2161 ctgggattag ctttgtttta gccagcacca cattttttga ttgtctcctt gatctacgtt
     2221 caactattcc acatcttcgg cccgaggtca ttgggcaaca ccgattcaat caaaacgtat
     2281 actaattgta ggatgtggat ttgcaggaat cgaagtttta atgcagattc aagaagcgta
     2341 tcaaaatgat gtaagtatag ctggaagatc taatcgtttc aaagtcactc atccaggatt
     2401 tggatggacg atgtttcttt tgttgacctg ctcaaaggca tctggtccag tatatataga
     2461 aatacagaga atttctttga agcttactgg actcgaaatc aatcgttatc tgctcagatc
     2521 gaaacccttt tccctgctat ctctgctgac acttaatgtt agtttgtgat ctagcaataa
     2581 ttagcacagg cggagctatt agtgagattc tagtgcttcg agattctatt ccagtaagat
     2641 ggatatcttt tttggacatg ggtatgtttc taccttatga aactaaaata cataggcaga
     2701 tcgtcaggtg tggctggtct catattgatt atactaggaa taatatttac attacaaagc
     2761 agacggatcg tcggaccatc atcttccttt atgtttagta atcctgattg gacagttaat
     2821 ggttccgttg ttattggcgc aggggtgttt atttttgtac ttggtatcat actctggcgg
     2881 atatcttccg aataattcag gagatggcgt atgagtttta atagacacaa acaattttcg
     2941 ccgataatgg cataaaggca ttaaacgcca tggatacaaa gtttgctttc tagattttgt
     3001 gaaactagcg cgtaaatcga actcatgact ttaagataaa cgagtctaag tgatgcgaga
     3061 gagaactgag gctatagcct cattcgctat tgctttcgac tctgctaatg gaaacctgta
     3121 tgtagctaat gctggcggta acaccatctc agtaatatcg ggcaaaacta atacagtatt
     3181 tggtagtcct atggtttttg attgatgagc aattgagtcg tgcgtaggtt atatttctaa
     3241 actaacgtaa atgcgtctct cttatgcttt tcacaacact tcaccgaagt tactttgttt
     3301 aatccctttc ctgactgaag attttctact gtgatgattt tgcaggaagc acatttgatg
     3361 ctagaattat tcatggcacc gaaatgtttt ggaaatcgta gagatcttgc gcctgcatca
     3421 tgccagtccg tagctccaac aataccaacc ccactgttgc gtagttcact gttaaatttt
     3481 gtgcaatgct actaccagac ggacatatga caggcggaac gtgtgtaacg ctacctgtca
     3541 acacgtccac tggcggtgga tccgagttgg tcgcctagtt gaagaattgg agtagcatca
     3601 cttgccagct gttggcactg ccttctcatt ttcatttcta tatatgaaac agaagccaat
     3661 tgtagcagta aatagcaggg aaaacagtag agtagcagga gaaaaaagat aagcagcgaa
     3721 ggatagcaag agtagcagag aagaatgttt aagtaccttc ttcactaaaa taactcagta
     3781 gtgattactg ttgctgagca gagaggaaaa agaaagacgt gtgatcgaac tctacgagca
     3841 aagtaaatct tctcgcgaga ttgccaaaga agtgcatatg tccttcggag atattgcttc
     3901 catcataaga agtcactctg gagaagcgaa agctgagacc aggaaagtgg aaggaagtgg
     3961 agtggtgtta tctaaagata cccaggtctt caatctcttt gaactgcaca agagtcccgt
     4021 agaggttacc attgaattgg accttaaacc tgacgaagta tctaggttat acaagcaatg
     4081 gttagagcta aaggatttac atgaggtaaa acaactttac gaagagagaa aacaactcga
     4141 cggcgaccta tcagaatttc ttgagctgca ttctctaata aaaacggaag gcgtaaagcc
     4201 tagacaactc ctgaatgccg catactatct atctcaaata tcagtccttg aagatggagt
     4261 cagggttcac aaaggcgagg ttcaatcttt agaaaatcaa aaacagagac ttataattga
     4321 attacgcagc tatggtagtg aaaaagctac cctactgcga gatttggact cgctttcttc
     4381 attgatcgcc agccataaac aggaaatttc ggatctcatc gatcggataa aacagctgga
     4441 gagtaatatt gctagattaa agaatacgag agaataccac acagtccagg cagttgctga
     4501 acagaaaata agagatgttt tagcagataa tcaattcata ttggtatcag cattgttcgg
     4561 agtcatccaa gctttgacaa aacagcctga tctgaaattg cttatctctt ggtcgctgat
     4621 atctcctatg ta
//
LOCUS       DAOR01000199           13179 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_16164, whole genome shotgun sequence.
ACCESSION   DAOR01000199 DAOR01000000
VERSION     DAOR01000199.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13179)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_16164"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4766..4855
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11985..12203
                     /estimated_length=219
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3863 a         2595 c         2604 g         3808 t
ORIGIN      
        1 ccccatactt ttattccttg gaaagtgcca actacctgcc tctttgattt gaattcagga
       61 tcgataggac cttcagcttt agaataatta gttccagtgc cactaagtga tatagtggta
      121 ttagcactac ctgcagtcgt agcagtagta gcagcggtag tactaatatt agcagtggca
      181 gatgtcacac cgggtctttg aactccagtt gtaacaggtt ttgggggagc agatatccta
      241 ttagcgagct gcgaagccat tgaattttgg cgtttgacaa cacaaagctg attatgaaca
      301 tccatcatgc tttcaagagt aagcggattg ctatgtatca aaaggcaacc acaatccgga
      361 caaacatatt ctctctccga aggcatgaga ttttctataa gaatagtact tatttaatcc
      421 ttcttagagc tcgatgaatg ccccatgcga aagacgaatt aaaagctaaa aaataaaaaa
      481 ttagttcgga atggggacaa gaaaagcaac aacgaaaaaa acgacaagca acagacctca
      541 gatttacaga ttgcgtccat cggttctata gttaatagct aagcattcca aaaaatatgt
      601 gaaaattaat agcaagggtt cttgcactag atttcagaga tgtcaggact tggtcatgga
      661 tactggggtg aagttatcga agtgttacgc tttgtaattc cagtgtacga caaagtaaac
      721 agggccattt cattgggaaa agacatgaaa ttccgactaa aaggaattca tggacgcgtt
      781 cacccgggca accttctgct ggatgccggc tcaggttatg ggaatatgtc acgaattgcg
      841 cttaacgaaa cgaatggcac atctaagatt atcatgtatg atcctatccc tgaaatgcta
      901 gctgctgcaa aacatttgtt cctgccagga ccctcggaat ttaccatgtc ctcaggagtc
      961 ttcgaatata tgccttttag gagcaatatc tttgacgcgg ttctttgtgg ctattcgctc
     1021 cgagacgcga ttcagcttcg accggccgtt tctgagatca gtagagtcct gaagctcgga
     1081 ggaagattaa tcattgtaga tcttggtaaa cctgataatc cattgataga ggcatgtgta
     1141 tctttttact tgaggcatat acttaagatc ctggcctttg cagttgcagg aaaggcgggg
     1201 ctaaaatttc aaactctcta tggtacttat tgtagatggc caaaaaactc tgagcttaat
     1261 aacctgttaa aagaatattt ttccaaagta gactttgata cggagctgct gggcggagcg
     1321 gttatcatcg cggcatacaa ataggtctca attacttatc ttaaatagcg accattgcca
     1381 cacaactgcc gaaattgtta aggttttttg ttcttgttta aggatatcct gaaaattctc
     1441 cttatcgtcg gctgaaaaat ggataaccca ctctattata ctccgagaca ctcggtgggc
     1501 agtacctgac gacgaccgtc aagggttagt aataggcgaa gttcgggttc gaatttttaa
     1561 ccgtaagtac atgaaactaa cgcgtaaagt taaatagaag ctctctcagc ttttttctcg
     1621 gtaattgaat ggtgaaagag gctaagcggg tccaagaaat atttgatgat atcatttcaa
     1681 agaagaataa ggtaataaca gaggaatctg ccaaagagat tttgactgaa tatggaatca
     1741 aagttccgac gtacgcattg gtcaagactg aatcagaagc cgtcgaaaag gcaaagcaga
     1801 tcgggttccc actagtcgca aaaatcgttt ctcctgagat tttgcataaa actgacgtta
     1861 atggagtcaa ggtcgggctt gcttctgaag cagaagtccg ggagacattt gtggacatgc
     1921 aggagcgcct ctcaaatcaa tacgaaatta aaggaatact acttgaaaag atggttccgc
     1981 ctggaatcga gctaatcgtt ggtttacaaa atgatccaca gtttgggcca gttattatgg
     2041 ttggggtagg cggaatcaat acggaaattt ttaaagatgt atcatttagg gttctaccga
     2101 tcacaaaaga agatgcaata gagatgatca acgatctgaa aggaaagcaa attttgaaag
     2161 gattcagagg ttcagaatcc gtggacatgg atatgttagg gaatgcaata gtaaagattg
     2221 ggaatcttgg aactgatatg gctgcatact acgaaagtat cgatttcaat cctgtgatat
     2281 catatcccca tgattattat gttgtcgatg caaaaataat tttgaaggaa aagccgcatt
     2341 cagaagccat ttccaaatcc cagccagact cgagctacat ggatgttttc ttcaacgccg
     2401 gatcgattgc attgattggc gcatccccag aagggggcaa agtgggaaat tctgtcttcg
     2461 aaagtctcgt gaagcatgaa tacaaaggaa aggtctttcc agtcaacgca aagggatacc
     2521 ctgaaattat gggtatcaag gcatacaaga gcctcgagga tataggcgag cctgtggaag
     2581 tagtagtagt tacagtcgat ctcaggtttg taccggactt gttgaaaagc tgtggaaaga
     2641 agaatatcca taacatggtc atcatatccg gcggaggaaa agaacttgga ggagagcgtg
     2701 ctgctatcga acaacagatc aaggaacttt cgagtcagct gaaggtaaga attatcggac
     2761 ccaactgcat tggaatgttt aatggcgaaa accgccttga ctgcgcattc caaggtcatg
     2821 agcggatgtt aagaccacag aatggtcacg ttgcttttct ctcccagagt ggaactgtag
     2881 gcattgcatt tatggaaaca gctgacgcct ttgggatgag caagatgatt tcatatggca
     2941 acaggtctga cgttgacgag gcagacatga tctggtacct ttcgaaggat cctcagacaa
     3001 aggttatcgg tctgtacgta gagggacttg gcgacggacg caaatttatg aatactgcta
     3061 agaaagtaat acatgaagca aagaagccgg tagttgtgtt caagaacggc aggactacta
     3121 gaggtgcaaa gcaggccctg tcgcacacag gatcccttgg cggttcgtat gcagttgtta
     3181 aaggagcatt agaccaagca ggaataattt cggttaatag ctacgaagag ctcaccggat
     3241 cgcttaaggc attgatatgg caacccgttc cgaacggtag tagagtcgcg atggttacga
     3301 atggcgcagg cccggttata gcagctatcg atcattttga agaactagga ttggtggtcg
     3361 ccgaaattag cgaacccagc aagagagcat ttaaggagca ttacccaccg acctatgtag
     3421 taggaaatcc gtgcgatatt acaggttcgg ccagtgcgga tgactatcga ttcgctatcc
     3481 aaaccttcat cgatgaccca aatgtggata tcatcatgcc ctggttcgta ttccaagacg
     3541 acccgttgga agagaccatc atcgatgtat tggcagagtt ccagaagcag aagaaaaaac
     3601 cgatattagt tggggcaatg ggtggaccgt ttactcaaaa gatctcggat caaatcgaag
     3661 atcacaacgt tcctgtctac cactccgtaa cttcttgggt aagtgcagct ggaagtctgg
     3721 caaagtggtc aaaagtattg acaggaacac agcactaaca cttgtcctgt tatcattatt
     3781 tttattgata ttgtcacgtt agtttttatt atattattat tagcatctcc tgataacagc
     3841 tctgtaagaa atctagttaa ttgtatagta cacaaaatgg agattaacga cgagcgaagg
     3901 aaaaggttat tatatttata agccgtagtt gagtttaatg gaaagcgcag aacaaagtca
     3961 ggtcgtcact gttagcccaa aggcggctca gaaggttgga gaatttatga agcaggaagg
     4021 caaagataac ctcttcttaa gagtatatgt ttcgggtggt ggttgttctg gcctctcgta
     4081 tggaatggga tttgaagaaa aatctgaaga agatgaccac atcattgaac aaaatggagt
     4141 gaaagttctg atagatggtt atagccaaag atatcttaaa ggcgcaaaca tcgactacat
     4201 cgagagcctg atgggagccg gtttcaaaat taataatcct aacgtaacaa agagctgttc
     4261 gtgcggacat tcgtttagca cagaataaag gtaaagtaat agtaaagttc gtgacatcca
     4321 atctcgaccg gtgagagtat cacttccaaa tactcattat gccagtcgat taaggccata
     4381 atcctctaat ttttattgca tcgactactc gatttatcgc taagactgtg ctagcttttc
     4441 gcatattgac ttggtattta tcatgcgtag tataaatgtc tgaaaatgcc cgtgttatgt
     4501 tcttgtcaag catctcgttt acttggtttt cgctccagga ttctcttcta aggttttgaa
     4561 gccattcgaa ataagaaaca gtcacaccac ctccgttggc aaggacgtca gggattacta
     4621 gggttttatt tttgtataga atatcgtccg cctccggggt agtaggaccg ttggcggctt
     4681 cggccacaat cttggcgcta attttcggag cgttcttttt ggtgatctga ttttcaaggg
     4741 ccgcagggat cagtaaggta caattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcagg
     4861 gatcagtaag gtacaatctg tctcaagcac ctcttcatta gtaatcaact tgctttccgg
     4921 gaatccaact accgaaccct ttttctcttt atgtttcctc aatgccccaa tctgaatacc
     4981 atttttgttg tacacacctc ccttcgaatc agagacagct ataacttttg caccctgttc
     5041 ctctaagaac tgggcggcga attggcctgc gtttccaaat ccctgtactg ccacggtggc
     5101 agtatgcatg ttaagcttca acttagtagc agcttcgcgt actgtaaacg ataatcccct
     5161 tccggtagct tcggtcctcc caagggagcc acctatggcc agcggtttac ccgtaatgac
     5221 ctcaggctga acatagtttc ccttaagtgc agagtacgtg tccataatcc atgccatctc
     5281 ctttcctcca gtataaacat cgggcgctgg aatatccgtg tgcggtccga taatatcagc
     5341 tatcgcatat gcataccgtc tcgtcatgcg ttcgagttca agatccgaca tttgttttgg
     5401 attgcaaata acgcctccct tgcctcctcc gtaagggata tcggctatgg cacatttcca
     5461 agtcatccac atagacagtg ccttcacctc ttcaattgtg acctgcggat ggtatctgat
     5521 accgccttta tacggacccc ttgcatcatt atgttgtgat cgaaatcctg tgaaaacacg
     5581 gatctcgttg ttatccaatc tcacgggaat tgaaacaata agaacccgct ttggatgggc
     5641 caatatctga tgtaacccat tgtcaagttt gattaacttt gccgcctcgt ctaactgttc
     5701 aagggctacc tcaaagggat caattgtcga aacatttcgg gaatctgaca ttatatacat
     5761 tctctgaaaa aattgatgta tttaagtgtt caaggtcgaa agttatggac tttataggaa
     5821 attgatcaat agacgtacac tatgttaaac ctggtttgtt cacgtattgc aaaatgacta
     5881 taccattata atcgtaaata attaccggct aattaaaaaa ataagtgaat tccaaaagga
     5941 aatgtcagac tatgtttaag acgtttaacg gtgtaaactt cttgaaatta atgagcgtat
     6001 ttcatcgtct gtcttcccca gacagtctat gtgtagtgat cgcgcaatag cctccagctt
     6061 ttcgtgttcc gaggcaactg gtccatcaat tctagtgccc atgagatgca ttttgttatc
     6121 ttccataggt gttgctgctg tcgtagcatt ttcaatttcc ttcctcaata attcccgggc
     6181 cctttgatat tcccccatgg cttttccgac tgtctttgat atctgtggga ccttctttgt
     6241 tccaaataat agaaagaggg caagcattgc aataattatc cactcagatc cccctatatt
     6301 catttgaaat gcggaaaaaa tgagtaccaa tatatttttc tacctacttt ccctgataca
     6361 taatatcaag ccaacactaa ttaaagaatt agctgaaata tattgctacg ccttttttag
     6421 gtctgagcca cacttgatgt cacgccctac tttcttatct ctccttagga cggcaaacga
     6481 ttggacaaag attcgcacat tgtcaattta aaattaaaaa gctgtagtaa acatgctggt
     6541 tgataatcta tggagaattg aactgatgtt agacgtggtt aacctttagg tttatctatc
     6601 cctcaggatc tacggttata gttggataca tcctcgtctt atcggagatt gaaaaccgga
     6661 agaaaaaggt ctctatggtg tttgtctagt ttttcagttc taatattgct aatggttgta
     6721 tccccattcg agtattattt ctttaatcaa aaagcatacg cagatggttt gagtcaagaa
     6781 aatctaccac ctgcttccat aggaaacagg caaggaagtc tttacgttaa ggtgaacccg
     6841 cctgttttga cgactggggt cgcacagaac gcatatatgc aatttaggtt attcgatgca
     6901 aacaacaatc agaccatcca acacgttacc tatcaaataa ctgtgactag gggatcttct
     6961 acatcaacgc ttggaaagcc cctattgctt gatttctttc acgcacacaa tggactacta
     7021 acattaaaga tagaaccaaa taatggtccg cttaccatat ttggagaaca agatcccttc
     7081 ctgcaagctt gggtatctga tcctggcggt aatatactcg taagaggccc cttactttta
     7141 cagggaggtc tatatcattt ccatattgaa atatttagca tagacaacga tagaaacatt
     7201 ttcatcccag cgcaagcacc gaaatttgat gcctatctta gcgtcggaga tgtttatcac
     7261 aacatttgga attatcaagg acaaaattat aatacaactc tgatatcata ctacgataaa
     7321 attaccaatc ttaatctaca gccagcagtc aaatcgttca cttggtcaat gccatttaat
     7381 tggaacttaa cacgtatcaa gcaacagcct atctttgttc acgaggagat gaggcttcca
     7441 aaatcttgga aaggctttgg tgatttgaat caattcaacg caacagttaa tggtcagcag
     7501 ctatcaggtc gttcgctagc tatcgatccg ttttccttcc ctaatgacat gattgtacat
     7561 tttcttgtta acaagaatga tatcgtaaaa cttgcacaag aagtgaattc taacaataac
     7621 aacagtgtcg ctgcaggtat aacgaagact aatacaacag gcctgatgaa atttgcttta
     7681 tctccactca acgccacgac acagactgcc acttcatcag atatccctac aaatactggt
     7741 agtattcatg ctgcagtctc gtggtctcca aatcctcttg cagcaaacac acaatcgaca
     7801 ttaaagattt cattctatga tcctaccgct acagctccat taacaaatac cgatgtcaga
     7861 tacaatctaa taatatttga taaaaataat caaccagtaa taaccaaaca aaatctgtta
     7921 gcaaagaatg cagctgatac agagacaatt gtttttccag caaacgaaat ttatcatatg
     7981 caagtgcaaa taacaggatt agttaagccc ggtcaaaccc cagacctcac caggaatgga
     8041 gtagcgacgg gatatgtcgt tgtgccggaa ttccattccg ctgctatcag cacgtcgttg
     8101 atacttgggg taatattagc tgggttgctt cctaggaaaa gcatcttcag aggactaatc
     8161 aaataagaag gcggctgtag aataatgtat tattgcataa ttcagtcgcg cactgctgca
     8221 atttttgcct actaatggaa acacaaatgt cagtcacaga tgaagattta ggcaaactag
     8281 cgtatcaact catggagcta ggctgaaaaa gaaaatacat cgaggtaagg ttctggatct
     8341 cagaaaaatg aagaaccgaa caaaatatgt aatgatggat tgtggcacag aggtaggcga
     8401 tcaacttcgc ggcctttgtt acatcactgg taaaaaacga cgattcattg aatagccccg
     8461 agaatggagc aaatcgagaa gttctctaac acaaaacgca gaccgacatg gttgtaatga
     8521 atcaattgtc ctcctgcgat cagaaatata gcgccaatga tctatggaat accaccagtt
     8581 gaatgtgcct tgtaataagg caggtgagaa aatgaaaggt cgacttctcc tctaagaagc
     8641 agataagaca gcaacaatat gtttaatatt tctactattt attctatagg atagtaactt
     8701 aaattaattt acgctgattg gaaattgtac agattcaggt cttattggat tgaagagaat
     8761 accatatgct gtgatgttaa gtgcgtatcc accgggttgt tgaaatgtat atggtatggt
     8821 gacgatacct tcagcagtat gtagtggttg gtttggatgc cctgccagtg ctgatgcctg
     8881 aaatacttgt ttgccatcct taataatagt gagatcgtaa tcaatgtggg gctgtactgc
     8941 actacttccc ttctggtcga aattaacttt taaattcgtt tgtgtacctt ttacaactgg
     9001 atacggagag ggttgaagaa taacattaag ggtccctgcg ctcgttactt tattcatcgc
     9061 agcgcccgat gtttgcgcat atgttgatga cggtgctagt aaaatcccga aaatgctagc
     9121 aactaaagcg aatgcaacaa tagtactact catataattg cgtctatcct tagctttgta
     9181 caacaggtca atatatattg tcacctcata aataggttga aatgtaaagc tttttgttta
     9241 gtaaatccac taccattatt ttattttcaa tattctctga gggttgtgcg aattaatcga
     9301 aaaatactat aaaatttatt ccctgattag aagaattgtg agctcaggta gtacctgctt
     9361 catcgcttca aggcgtgcct tggcctgttc attactatca agatcatgaa aattataatt
     9421 attatattta gagctacaac tcccataact ttgttcctgt gatctcagca cattctacag
     9481 gaacaggttt tcttaacggt acgaatgacc cgagaccatc cgatttttgg ccgcgcctat
     9541 tagcggaagt ttgtaaatgc atcaaccgaa ggtttgcagt gagatcaggt tcgtattatt
     9601 tgttgattct cgtgtcgttg tctaacatcg tcatataatc tagattatcg tctttatgtt
     9661 tttctacatt ctttatttgt aaattctcgg catttatgat catgtctcca gctcccgttg
     9721 aagaaaattt ggagatgttt tacaccctct ctgtcttcta taactcttgt gtaaccgttc
     9781 tttggattgt taatgtatcg ttgtctgtca gaaacttcgt tatatctggt cataataata
     9841 ataatgatgc taccgacata tatgaattgt atataggggt tatgagcagt tccgtcctaa
     9901 aagtacctct aattcgaccc aacccccaca agtgatcaca aattgcctgg acagcgatct
     9961 gcgatcagct tgtggggggg ccagcgatct ctggatcgac taaattatca ttaaattata
    10021 agagatgcga aaaaatcttc gttatttcct ttttgacctc tggtggtatg tttgaatctc
    10081 ctattatctt gtggagatca tttctattaa tgcatagtcc cgggtcgcag actttataga
    10141 accatttgat atcaattgga cttattataa cagttgattt attacatcct ctcattccac
    10201 atgtattcaa atcatcattg ctgaaacata tatgaccttc ttgacattca tatatcatat
    10261 atcatccgag ggtatagttc cctttgttaa agactattct atcgttagtg agtttgatac
    10321 attttattgg aaccatacaa atgttatagc aatcagccat gcgaatgaag aatagtcaat
    10381 gtatgatcat ttgacacctc taagctcaaa agtcaaagta ggtcaagctg tcagcgaatg
    10441 ccaagagata gccggatata gatttatacc acatcttcac ttccaggctt tcatttttat
    10501 tggaagtaat gtttggacag attttgatac ggtaagtgtt caaggtttta tctcgtgaaa
    10561 ttatttatct aaccatggca tacatggcag aactagcgtg aacatatcaa atgaaagatt
    10621 ccttggtaat ccttctacag atttaccaga aggaatcgac tgtacatttc tgttcatcta
    10681 atgcacgcgt ttgaatggca ttccagaata aacagtgtat cttttcatct tgatttgcat
    10741 tattatcaaa tcagtacagc caagattcaa aaggagagag ttaacacgtc agacaagtat
    10801 acagtagaat agctacgtca aggattcgcc gtggacataa cattctctac tttaatttgt
    10861 agttccagat ttatctccat gcggggtttt agagcgcaat atttcctctt ttgcttactt
    10921 cacattctta gcacatgcat cgcaatattt ttctactaaa ataaccccat cgcccacatt
    10981 aaacaaagct tcctgagtgg ccttagtgcc acaagttaca caaaacttag gctttgaatt
    11041 agggtagtta ggcttgtctt cgaaagatct aagatgtata aaatttttct gcatctctaa
    11101 gtccatacct cacaagctgt gatgaaacct cggctggaat agtagcaaat gtcattcatt
    11161 cataaatgta tccttgctac ctataagaac ctgcctattc acgatctaac tgtatcataa
    11221 gtttcttcta tactggtgct catacacgcc tacatatgga tcagttattc aaaaaaacac
    11281 caaccggggt gaaccagata gtgtaggatt cggtggttct ggcgacgaca gtgcttctat
    11341 aaactgatta gttcttcctc tttatcattt aaacaaacat tcacttagta ttacaagata
    11401 tttttttcat ctttattatt ggacgtctgt attctaggaa gaaaatttat taattttgga
    11461 agtcttcgat cctttaatta ttggctcgtg attatcaaaa ataacatcaa agacattccg
    11521 actatattta gcgtcgtcta ttggaattgc attaatattc tacgggtatt gtttattata
    11581 tttttttaga taaacaacaa catttagttg aggttgttga ttgtctccga accacaatct
    11641 atacctgcct tttagaaccc tcttatgatt acaatagagt catcagtcaa ctgatcagct
    11701 aaatcacccg gttcaatttc atcatatccg aatactctta aagttcttgc taagattatc
    11761 taggatatag cacccgtcgt ctgtttgtga tttaactttt gttacgtaat ttactattaa
    11821 gcttaattga gaacggaccg tgatatcaga aatttcgttt attagaattc caactgacct
    11881 aatgaggttt agctcgatat aactaagtac ctcaatgtaa taacactaaa gatgtaaaga
    11941 acaccaggct cccatactct tacttgttta catacttgtt tacannnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnacttgtt tacatacttg tttacattgt caactatacg
    12241 ggggctcaaa tcagcagact tggacagtct ggtaacctct aaggtacccc gtgtttgaaa
    12301 atcgtaccaa gttacctaag gtaaggtata atttcaattc tgcccacaac ccgactgatt
    12361 cgaacacgcg cgccaaatcg cctggtttgc gcgaaagcct ggttttttcg catagcgaac
    12421 tttaagctac tactattata gcttttgaga acctatccgc tacttcagtg accttaacct
    12481 taactttctg cccaactttc ccatctttga cgaatactac aaatccttgt acccttgcaa
    12541 ttccgtctcc ttgtcgactg atttctgtaa tgtctacctc atattcctta ccaacttcga
    12601 ctggttttgg accaggagaa cctccgaacc ctctactacc gccaccacca taactcctgc
    12661 ctctaccata actcatttgt agacctgatt ggttgtcgtc ctaatataaa ctggctatat
    12721 cggtgagaat aaaaaaggag ttttcgaagc aaatcttgct tgaaaggcgc attacgttcg
    12781 agttataaaa gcagtggcaa taaaggatat cgaaaaatta gttctattcc agatagacca
    12841 gttccagaca atgctagcac gaaggccgag ctttatatac ttgaatcgac gtgtcactgt
    12901 catagcttat ccactttact cctcattaca ataatgcaag atccaattgt atgctcaata
    12961 tgtaaaaaag atcagacgat catcacagac gtagtcaccg gtgagatagt ttgtggtaaa
    13021 tgtggtctag taatttctga gaaaattcaa gacacaagac aagaatggcg tgatttcatg
    13081 actactgaag aagataaaga tagaaggaga acaggaatgc ctacctcttt ggctcgccat
    13141 gatatgggac tatatacaat gattggaaga ggtcataaa
//
LOCUS       DAOR01000200            5166 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21797, whole genome shotgun sequence.
ACCESSION   DAOR01000200 DAOR01000000
VERSION     DAOR01000200.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21797"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4363..4493
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1356 a         1054 c         1085 g         1540 t
ORIGIN      
        1 ctgcaatcaa gtctggagtt gtgacatcac gtacatccgc ctggcgcggg ggtttatcta
       61 tctcatggcc gtcatagatt ggttcagtcg ctacgtcttg tcgtgggaaa tctcggtcac
      121 gttggatatg agtttttgcc tagaagcgct ggatcgggcc ttaaagatcg ccacaccaga
      181 gatcttcaat acagaccagg gggtgcaatt tacgagtgct ccgtttacca atcgactcaa
      241 agcagcggat attcgtatca gctgggatgg ccgaggacgg gccttagaca atatttttgt
      301 ggagcgatta tggcgcagtg tcaaatatga ggaggtctac atcaaagatt accaaagcgt
      361 tccagatgcg gtgaacaact tgcgagcgta cttcacgttt tacaatcacg agcgtctgca
      421 ccaatcgctg gactatcaaa cgccagcaca ggtctatacg agcaaataag acgagtcttc
      481 tcgaaccgtt gtgttttgtc ttatttttag cccaaaactg tcttgacttt tgggaccgcc
      541 ataaaatata tcacggacaa tgtgaaggtc tttgatgcgc acttcttata agaggcaata
      601 tagctagtct gagcgatagc aatggataag aaaaagaaca aagaaaaaac aatttttgat
      661 ttagtttact ctggccgagc atttgactca gttaccccca ctgaagagcc tgattttaag
      721 gtaaaaaatg cagatggtga gtttggcgtg gagataaccg aattttactt ttctcaatca
      781 gaagcgagaa tgaagaatat tcctggatat tttacggaaa tcttagaaga aaaaaaatat
      841 agacataagg atgatgtcat tccttttgag gtgaaagagt tcacgattct acctggcgac
      901 aatagaagac cgagctttaa agaagagggt cttctgcgac aattgccaag tattgatgag
      961 tatgtaaaga aaatttcgga gttgattgag cataaaaaca agcggtataa aaactatata
     1021 acgggattaa atcacgtaaa tttgattatc ttggatggcg aacgtcggtt attgggcact
     1081 ccaaaagatg cgttccacca tttgtttttt caatctgagt tagagaaaac gctgatgagt
     1141 gcagacttta gagaggtttt tttcgtaact gagcttggag agtttgacgc atctaaaaac
     1201 gtctatatcc cgctaaaaat gttgtttttg gtcgcggaga tattcttatt gaattatatt
     1261 ttggtcaaag aataccctga tactctgatg acaccgcttt tgtgtgctga gtatttactg
     1321 tggagaggcg caaaagacat ttattttaaa cgtgcctctg atggttttga gatcgggtat
     1381 ggaaactctg gaatttttat atcaaacgac gagagtgagg gtataaatat aagagactat
     1441 agtgactttg ccttaccagg agattttact tccattacaa catcgcgggc atctagtttc
     1501 ttggatgaca cttttttgag atcatttgaa aaatataaga gtgattgtgt tttcagcatg
     1561 gagttgtgtt ttgatgtgag tggaagcagt tagttggctt tggatgaata aattcttatg
     1621 ctgcatgggc ttgttacaac aggtttttct tgctgtcata ctttgaggct ggttcgcact
     1681 gaaaggccag atcagttatt gcttactctt gtttgaggta agagaagcta aaataactct
     1741 gttaaccaat gccttgactt gcttgatttc ttgcaacgat ggctcttctg ctttctttcc
     1801 atgctctatt gcatttctta tttttctaaa tttatctaac agtagttcat gctcatgttg
     1861 actaagcact atcccaatat tcgtaaggtg ctgtttaaat ctctcaatca aggctccgtc
     1921 atttagcctt ttgattgcac cctgaacaag attaccctct tgctcggtga ggttagccat
     1981 agcacgagac gataagtcaa ttagctgctc ttgggtatag cttttcggca acctgacacc
     2041 ttttgacaat aattcaacga cctgccatag tccaatagta accacatagt aatcttcact
     2101 gtctctaaat ctgttccaca caagtaaagg aagctgaaaa ctttcactag cgtccatgac
     2161 cgatttaatg tctaacttgc tggtaggctt tagcttggtc tggattggct tgtacggaga
     2221 aaggtctcgt aaccacacgc tgccagtgcg cagcattact agcaggatat ggttttgacg
     2281 tttcaggtta attgctccgt cgatcctttc ccattcaata aagtctcccc caaattggga
     2341 gtagctatac tgcaacaagc tactataggc agataaaaac acatctgcct tttctattgc
     2401 tatgttttca gcgtcggcga aatgtgccgc agtgaaacga aaagaaatcc atccatctgc
     2461 gtcaagaaat ccatgccaaa tttcgctttt ctcattctta ggtaattttt cctgcatact
     2521 cttaacgctc ttcagagcaa gaaactggac tccactgatc atttcatcct gttcaataag
     2581 taagccttta aatggcacaa atacgatcat ttccttaagg gagttgtcca gcccaggtat
     2641 gttaatatga tcttgagaga agcctgccag ccgtgtcatt gaataggcca cttctcgagg
     2701 atcgacatgc atcacagata gagacttaat gcccgtttca gtaagctcta ggccgttatc
     2761 aagcattatt actatctgat tgtttagctc gctttttatt gaaacaatgt ttccagtata
     2821 aagacggcgt ttttggctga ccgagttaat caccacctcg tccaggtaat ttacggctcc
     2881 aagctggaga aaatcgcttt tatcaaaacg gagcatggca gtagatgaag cgtttatctt
     2941 ttctgtgatt gtacaataac ggcatgtcac tgatgaaaca gtgccaataa ttacttctat
     3001 ttcaccgacc atgtggctct ccctctccta ctttctttga aataaaaggc cgtatatagc
     3061 tagtttccat atgcctagag caattgggat taccctgtcg ataaccctag ctctccctgt
     3121 agtgcgtctg cctgttgtac tcgttgcgct tgcttaaatg tgccaaattg cgggggcatt
     3181 tttacttctg cattgtgcaa aagttcttct atagtaagta tctggatgcg tggatagtct
     3241 ttattccagc ctggggaatg gtagaaacct gctttgactg cctctgttgt catatcgcgt
     3301 gtaggtgctt cgagggtgat atacacgcct atagctgctt gctctcgttg taccgtacct
     3361 acaaggtcac gaatatggct tgaattgaca tgacctgatt tcacctgcac cagtacccgc
     3421 tttgcttttc ctgtgttgtc gtctataaaa gcgatcactc catctatgcc tttatcactt
     3481 ccttttttac cctctctgct tccttcctgt cctccaagtg gtcgagcacg tataagagat
     3541 aaagcccacc attggaactg atagcggtct tcactggcaa gttgacgggc tgcacctatg
     3601 tcttttggct ctccaacaac tttgaatttt gtacctggga acatggcttc aaggcggtat
     3661 ttttgcaagg caatactcag atgggttata tcaataccaa tccacttgcg gcctagcttc
     3721 tgagcagaag caatagctgt accgcatccg caaaatgggt ctaatatcac atcgccagga
     3781 ttactacttg cttcgatgat acggtctaat aaagctaaag gtttttgagt aggataacca
     3841 agtcgttcat tgccacctgt cggcgtaata tcattccata ggtcttggag gggaactcct
     3901 ggcatttcat ctaagtaacg cttatagcga ggcatacctg tactcgtata tactaagcgt
     3961 cctccctgtt caaattgctc catttttgcc ttagagtatg cccaataacg ccccttgtag
     4021 ggtcgcacgc catgccactc atattgcgtg tctccacctg gctttgctgc tgtgaggtta
     4081 tctagttggt aaaatctacc tgttcctggc tctacgtggc ggtatgctgt ctcgatatat
     4141 tctttgtcat atggtaagta aacggggttc catgtccatg actggctctt tgtgtagaat
     4201 aagataacat cgtgaatatg cccatactct tttcgtccct gtttcgtgtc actgtgagcg
     4261 gtggtacgct tccaagcaat ttcagtgtga aagttctggg ggtcaaaaat agtatcaagt
     4321 atcaccctga gatagtgact tgcagttggg tcgcaatgta aannnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgcaa
     4501 tgtaaatata aactgcccgt tagtttgagg acacggtgta actctatcag tcgaacagtc
     4561 atcatcacaa ggtaagctag catctgattt tcaccaatga aaccacgaag tgactctatc
     4621 atcttagcta catggtcggg agcttcggta agcagttctg tataggtatg ctctgctgct
     4681 atattccagt gccaagtatc ttcaaacgca gtgatttgag actcgctatc tatacccttc
     4741 tcatccttaa acagcacatt ataatttcgg cttgaattaa acggggggtc aaggtaaata
     4801 agatcaacac tctcacttgg tatatgctca cgcaaaataa acaggttatc cccgtagaaa
     4861 agggtattct cagaaatagg ctttggtgac atgctcccct cgctaactgt catatttctt
     4921 agcagtataa catagagcgt atagaagcac atcacatata gctataatgc tgagtcgcag
     4981 aatgaaaaca aggcgacatg agttgttttc tatgcagcag cttgttgaac cgttttacgt
     5041 atccgtacct ttttggctgt gataacaaga aagtggtgta actgatcggc atgggtatga
     5101 aggacagaag caagcctgtc ttttctcaaa gcaatgttcg tttcctctga tttgaaacga
     5161 aaaaga
//
LOCUS       DAOR01000201            3377 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_43224, whole genome shotgun sequence.
ACCESSION   DAOR01000201 DAOR01000000
VERSION     DAOR01000201.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_43224"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1137 a          607 c          603 g         1030 t
ORIGIN      
        1 ctggcctcat ccaggaggat gaaaaaagaa tatccgatag aaagagaaca aaagaacaat
       61 acagtgaaca agcagcatac atgtcattaa ataagaatct aatagagcct ttgcaaaaat
      121 attggagatt tattactgaa actaaggttg gaacacatca ttttatctct ttaactgatg
      181 agggaaaaaa cgctttgcat ttccttagtg caggtatttg aaaatccatg agcaattcta
      241 ctcatattgc acctataatc gagtgtatat ctgaacagtg gtaatgaaca ttgcattttt
      301 aagatcatgt caaggtaata attagaaacc aaaatattct atctagaatt atttcttatc
      361 ctggtaaatt tgtgaccact ttggatatag aggatggtac ttctttgtgc cctttataat
      421 cctgattatt ctatcttgtt cttctcttct cgttgtaatt gcctcagcct ttctctatct
      481 cttttattag ttggtgacac tcgtaatgca gcaacaagga cagaacacgc cattgcggca
      541 atagtaaacg tcacaccttc atagtaactt tttcgcaaaa ataattttgg aatacaacct
      601 ctcacataga tttctacaaa ccatggggaa ttgtaaggaa aaggttggac tgttaaaaat
      661 attggagcta gttgcagaaa gtgggaggga aggcagcaag tgtattcgga cagggagtgg
      721 cgtaggacct taagttacct tttacagcgc gataagggtt tgataatgaa gtgaaaattc
      781 gataagggaa caacaagcta tgactacata ttcgaggcat tcgatcatcg atgcatcgct
      841 gcaagataat ctactaagta gagttataat cggatctact agtgcactaa cacacagtcg
      901 tcagggctta tgccaatcag aaaacttgtc tcctgatttt actttgtaac tgatctgctt
      961 taaaagatat ttaataagca taaaatattt tgctatgaaa gcaagaataa ttgaaatcat
     1021 tccaccaatt agaaacctta tgccgatgat cttaaagcct tcaccgtctt ctttaggtga
     1081 aacgacactg gtacctatcc agagacacac aacaccaaaa agctataatt attattcctg
     1141 cagctagaag tggcttgaat agcattgtat tttcgtttac agaatttgac attgccgtct
     1201 cgtatagaat gagtggctgt aactaattaa atggcagttt ttcagcagca tagattgatg
     1261 cgtgacttag tttgatgata acaccaatca ctgtatatgc aagaccactt tcacgataag
     1321 tcccatcttt cttgacagta gtgtccattg ctacaataat accatccctc atagtgcgaa
     1381 cgagggggaa tatattttcc ctattcgctc agtccttttt gtttacaaaa tgtgcaaatc
     1441 gctggtttag gcatcttatc tacctatact aataacaaga agtctgttgg tattagaaaa
     1501 cgttctcgtc gagtgctagt agactcggat atgtcattta attctagatg acggctctat
     1561 taattgaatg tatattatta tggtctaggc aaacccatca ttcaaagcca atgaatagac
     1621 aacaataaaa aaagtgaatg atctatgagg attgtaaaaa aaggatttat ttctagctaa
     1681 gactacaaaa tgcatcgaaa atctctggtc ataagagatt tgataaaaca gttgcctggc
     1741 aatattgata attcataaat gggaacacta gtactaaaaa ctgaataaca ttggataaga
     1801 aaaacatata cacaactgat gaaagacaac cgaaaggaga acaacatgat ttttatgtaa
     1861 acaaattcat tggcagttat gaaattagac gaattttgaa cgaatcagat tccatggagc
     1921 aggagatgta tcatgttacg ttcaaaaacg gaggaatgac aaaaatacac atacatgaat
     1981 ctgaacaagt actaattgct acaaccactt caccggattc tgacacttca tttttagggg
     2041 aaggttttgt tactctcata cagaaaaatg tcagtacaaa aggaggtgat ccaaaatatg
     2101 ctatcggtaa gacaattttt cttaataaga ttggagatac agtttgtatt ccgccaaatg
     2161 tattacattg tcatggatca agaagcaaag aacaggattt ttcacatgtt gcgattagga
     2221 gatcaacttt agataatgga caacaagcta aatcgctatg ggaatacgat attaagacaa
     2281 tgaagaagat tatgggcatt ctcgatgatg atagcaaaat agaatctatt ctcaatgaat
     2341 tacataatga aattaaaact attatttccg aggcctgaaa tgcagtctat aagaacataa
     2401 actctaagac catgcatcat gaagtaaccc taatagtgaa caatccaaaa tatttctttc
     2461 tggatttgga ttagcgaatt aaatctcgat aacctccgtg tgcttccaaa tacctgtgcg
     2521 acgcattatg tattttattt gcattgaatc attttacctc gtaaggacgc acaacacaat
     2581 tataacatca gtaataggtc agcgagaaaa ctatcttatt tagtggtcct tgcagtgact
     2641 ctaaccctcg aagatgcccc gtgaccaatt atgaatttgt gattgcaatt tgtgcagcaa
     2701 tagggttggg catagccact tttcttatgt cggaaacgag gctcaataat tgaaccgtca
     2761 gtttgatatt ttccacagat actgaataat taaactgcca tcccattttc ttataacatt
     2821 ggtaaaccgc caactgacca accgaagcta ggcttaaatt ggattgtgta ttgtattctc
     2881 tgtaatcaac caggtactac ccgggatagt cttagactgc accttaacaa gaaataatct
     2941 cagaaatact tgatcgtatc aggtctcaga atttgtttta gaagaaacag ctcccttatt
     3001 attaatagat gacgctatgg ttcattactt ttcctttaca aattctattt ctatacccct
     3061 tttgttgtca atgcttattt tataactctc atagtcggcg acaggaagca tctctaaatc
     3121 tccttttccc cacgtaggac agagctaaaa attaacacta gcagagtctt ttagtaaaaa
     3181 tgcagcccat aaacaattgt tgcggattac taggtctcga tattcatgca ttaccaaata
     3241 tctttatttg aagaactatg cagtacgcta tgtcatttag gttgtcttag taagcatgat
     3301 tcatctttat gcttagatgt agtacttaca tagataggtg acactttcaa atacccacat
     3361 aggtgacact tttctgc
//
LOCUS       DAOR01000202           10857 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_62306, whole genome shotgun sequence.
ACCESSION   DAOR01000202 DAOR01000000
VERSION     DAOR01000202.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_62306"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2925 a         2261 c         2339 g         3332 t
ORIGIN      
        1 aggttcgtta tcaatccatt gacatcctct acatcgtcaa catcctcctc gcctccttca
       61 tcttcacact caccttcttt tccttcaaaa tcaaatacac cagcaacaac agcagcaggc
      121 ttcataaagg ctatagaatc aggttcaaaa caccttctta tcaaaagatt cacattgcgc
      181 tatccagaac aaaaactcaa gttcgttggc gatggctatc aattcgatcc aaagcgagcg
      241 gtgggtattg ctggtctacg gggcagacac attctatatc atgataaatg taccggatgt
      301 cagctctgtg caattacgtg tgaaggcatt gcagaagcca ttggtatggt aaaggtggac
      361 gaacagtgga agcataacaa gaaagccatc atgccccaaa tagactatgg caaatgtgta
      421 ttttgtgggt tatgtgtaga tgcctgtcca ttctatgcat tgtttatgac aaatgactat
      481 gaactctcat catttacgaa ggaacacttg atttatactc cagcacaatt agccatcaag
      541 ccgaagtata gcggtgatgt cgaaataaaa attgacgatc atggtgcttc acatcatggc
      601 tgatgcttta tttattagcg ttgcaatcct gactataggt tcggcaatag ttgcacttga
      661 aacgcgggag cttgtctacg gcgcaatcgc ccttgcaata tctatgatgg gaattgccgg
      721 cttctttctg cttctagatt cgccatttgt tgcgatgttc cagatcacgg tatatgttgg
      781 agcggtggca gtactggtta tatttacagt tatgctagtg cgaacccaag cccttttttc
      841 aataaaagag gaccctagaa gaaaagccgc agggattgtt ttactgctcg tatttatcgg
      901 aggaatgggt gctgtagttt tagcctcagg tcttggcaat cctgcattta ctaaatttaa
      961 taaacctgca attgatgtcg ctacgatagg taaacagatg gtgacttatt acgctccggt
     1021 tttagtactg ttgggactta cgttagctgc ttctataatt ggcgcgctag cgttggcacg
     1081 aagggaggat atagaacaaa aaaatgaacg agttaattga ttttgttact gtggctgttg
     1141 tcctttttgc aattggtata tatggcctgg tcgtcaagcg taacgccata cgcatgatct
     1201 tttcgataga aattatcgcg aatgcggcta atctaaattt gattgctttc tcaagaaatt
     1261 tgcataatgc ccaaggtcaa atgttcgcgc ttttttctat tgccatcgct gctgcagaag
     1321 tagcggtcgg gcttggtatc attatcattg catataggtt atacaaagag atcgatgttg
     1381 aagaatttag gagtatgaag ggctaggtat caaaaggatg gcagagtcgt ttttcctttt
     1441 aatagcgacc tttatgcctc ttttgttgtc gccagtcgcc tatttcctag gtagaagagc
     1501 tggtttaggt gcggtaacat ggttttcttt tggtgttttg atcatatcta ccggcctgct
     1561 ctttgtacct gcgtctatgt tgaacggagg acgcaccgta tacagcgaaa gttatccttg
     1621 gggccaattt gggaattttg gtcttagact agatggtctt agtcttccat ttgcaattat
     1681 caactacata tggtttacgg ttcttataat ctattcaaag ccgtatatga atcgcaaaat
     1741 aattgatgaa gctcgtagtc gttttggaga ggggaagagt actgaaagca tatcagtagg
     1801 tcgtggcccg gcctatgcgg cagatgcgga aagacgaaat gatacttcag gcatttctag
     1861 cactgagagt ggctctttaa caacaacaac aacgacacgg gtcgaagaac aacggtatgt
     1921 aaactcacag ctggggcttt actttgcact attcatggta tttgcaatgg gaacccttgg
     1981 tatggtcctt gcgaccaact tgatcgagtt ctacgtcttc tttgaattag tccttgtacc
     2041 ctcattcttt cttatagcgt tttttggcta cggttcaaga caaagaacag gtctgatgtt
     2101 ctttttctgg acgcacgcag gctcagttgt actgttgctt ggtttgctat caattggttt
     2161 ctttgcggga ggttttgact atagtacagt cagggcccac gtatcacaaa ttcctgcaca
     2221 atggatgccg attataattt ttgctcttgt attggggctt ggggtaaaga taggtggctt
     2281 tttgttgcac tcttgggttc catcctctgt agccggagca cctactccgg tctccatcct
     2341 gttagtaggt attgctacct acggtatttt acgaatatgg ttggaccttt tgggtggcaa
     2401 ctacgtccaa tatagtcttt atatcaacat ctggggtctt gccacaatga tctatggtgg
     2461 agccatggca ctgatgcagg acgatatcag gaaagttctt gcttattcaa ctataagcaa
     2521 tatgggttat attttatttg gtcttggttc cggtagcgtt cttggagtca ccggagcaac
     2581 attgctgtat gtaacaagcg ggcttggcaa ggcaatccta ttcatgatgg cggggtcaat
     2641 tagtcttcag acaggcacta ctagcatgtc gaagttaggc ggtctcgccg gtaagatgcc
     2701 gtacacagca gtcctggcaa tgattggtct tctcacactt tttggcattc ctcctacaag
     2761 cggttttatg tctgaatgga tccttttcaa cggtgtatta cagacagggg ttcacgacgc
     2821 gaattcactt aggattgcat tatttggatt tggtatgttg acgactgtgt tatcctccgc
     2881 ttatttactc tggatgtaca agcgtatctt ctttggcaag ataccgcaag aattggttaa
     2941 catccgagac tctggcaggt atattacaat aacaatggcc gcgcttgctg gattgaccct
     3001 tattatcgga gtctatccgg atccatttct gactcctatc actacataca ttcaaggaat
     3061 gttccacaac tcaccagccg tacttccgtt gccgtcgtca ggagggtctg gcattgcagt
     3121 tccactcgct aagaacataa tgttcaaatc tggtaatgga ttcagcagca acgctgtgcc
     3181 aaattctgct gcacaaatgg aaagaattaa cagcgggggc gcgtttgcga gtcactaaag
     3241 aaatgaaata tgatgtttac atggtaggtt gaaaaaaaag tggtcgaaat aacaggcttc
     3301 gggggaatcg ccgtaaatgt ttggctagtg tggattcttc ctttcatcgg cgcagctata
     3361 attgccgcag ttgctcgtgc tggcatcagg ataagagatt atactgctgt tggttttgcc
     3421 cttgccagtg cggtctctgc tacaacctta attccgcttg cattctctgg caacgaagta
     3481 catagccagg taaattggat tgcagttttg cacttgaagg ccggggtatt gggcgatcca
     3541 ttgtcggtaa taatggccaa tcttgtggca tggatctcat ttttgatatt cgtatattcc
     3601 acaggatata tgcacgggga aagagacctg acaagatact ggttttttat gctcttcttt
     3661 atcgggtcca tgcaattggt cgtgttatca gataatttcc taatgctctt ttttggttgg
     3721 gaaggggtag gtcttgcatc ctatgctctc gtcggctttt ggtatactga cagaaagaag
     3781 gactttgtcg gaacagaagg ccatcgagcg ggaggaattg cgatgtgggc ttctccatct
     3841 cacgccgggg cgaaagcgtt cataatgaca aaagcaggag acaccatgat gcttgcagga
     3901 atgtttctga tttttgcata ttcaggaaca ttcgggttta aggaattact ccaaaatcag
     3961 ggatgggcag tgcagatggc gcatcagaac ctgttagtgc ctgcagcaat attactattt
     4021 ggtggtgcag taggcaagtc tgcacaattt ccattgaacg aatggcttct cgaagcgatg
     4081 acaggtccag cttccgtatc ggcgctgatt catgcagcta caatggttaa ggccggggta
     4141 tttctagtag cccggatctc tcccttgttc tttgcacttg cagcatttgg tatgacacaa
     4201 ttctttgaag tggttgcatg ggtaggggct attactgcat ttcttcttgc atcacaggct
     4261 attgcgaatc cagaaattaa gaaagtttta gcgtattcga caggctctca aattggatat
     4321 atgatgatgg ccataggcat agcaggactt tccactaact ttgctgaagg ctatactgca
     4381 ggattctttc aattgataag ccatgcgatg ttcaaagccg cactattcat gggcgcaggg
     4441 gcactgcttc acgttgttgg ttccaggttt atgaccgaca tgggcgggat gcgcaaatat
     4501 atgagaaaaa cctacatctt tatgttccta gcagccatgt ctctcgcagc tgcaccttta
     4561 atcacattag gattttggag caaggacgcc atatttgcag ctgttttggg atcaggctac
     4621 gtccattcac ttccactctt cataatggct ttcgtagtcg cgataatgac tgccttttat
     4681 acatttagaa tgatcggtat gtcattcttt ggtaagaaga gccctcatct ggaggaacta
     4741 gagaaagccg ggcaccatcc tcacgaggta cgcccgataa tatgggtccc ttttgcaatt
     4801 cttgcagtag caaccattgc tataggagtg gtcggatttg cctttggtgc agagcttcag
     4861 catatattcg cagattatct tgctaaatac tttggaatca cccaacaaaa acaactgcag
     4921 ctgcttcaaa acccagcaac aaccactcag cttgttaagc cacacacaga gaccaaacta
     4981 ttccttggtc ttaatcctta tgctgcagct gcatcctttg gagcctttgc gataggaggc
     5041 tttttgggat atatgtttta cattgccagg aaaattgatc cgcagattat ctctaagaat
     5101 attgttacga ggggaatatg gaagttcctt tacaatagat ggtaccttaa ttcagcgctt
     5161 tattggggcg ctgtcatagg gcccgtagca atctatcgta taatctggag gtactttgaa
     5221 atttctgtca tggaaggcat aagccatgca tttgaactct cgatggcatc attctctaga
     5281 gtagtaaaag caggacagac tggaataaca caaacttact tgtttgtatt cggagttggc
     5341 attatgatta ttataatgct gctcttgcta tagagagatt gttgaagaat ggttgatttt
     5401 ttttcgacgc cccttctcgt gacagtcgta ctcggatccg taggactcgg aatcccagta
     5461 atagatgcgc ttaaaaaaga aaggggttct aataacaaac tgtatagtgc gattgccttt
     5521 agtgcgctaa tcatagcgat aggcattgtg atcttccgtg tattatctgg ggcagtgatg
     5581 ccagccgctg catttgggac aggtgtcctt tcagatgatc tttttggttc attttttgca
     5641 gtggcgctgc tgattgtctc aatcatggta actgcatctt cttgggatta tatgaaaggg
     5701 agagcaaacc ctgctgcgta ttactcgctc atattacttt caagcatagg tatggtacta
     5761 attggttatt ccactgatct cgtaatgtta tttgttgcat gggagcttat gtcactgccc
     5821 acctatgcac tcgctgcctt ttcaaaacga gacccaatct caaatgaagc tgcgataaaa
     5881 tactttatgt ttggtgcatt atcttctgcg atcctggttt ttgccatagg gcttgtttac
     5941 gggattacag gaacaaccaa tataggggga gcaatccaag gattggctca tcttcaaaaa
     6001 gacctaattc ccgtaggact tctttcgatc gcattgttta tagccggttt tggatttaaa
     6061 ataggtcttg ttccttttca tatgtggctg cctgatgcat atgagggtgc gcctactaca
     6121 ataggcgctc ttctagctgc cggaacgaaa aaagcgggat tcgctgcagc gctgcgtgta
     6181 attgtgctcg gaatgttcgt attacatcca gactggacat tcacccttgc tgtaatagca
     6241 atatttacaa tgacactcgg gaactttgcg gctttggttc aaaaaagtgt tccaagaatt
     6301 cttgcctatt ctagcatcgc acaggcaggc tatatcttga tcggctttgc gctggcccca
     6361 tactccaacc aagctctgac cggttctctt ttttacataa ttaatcatgc agtaatgaaa
     6421 tctgctgcat tcctagctgc ggctgctgtt gctactgctt tggctggtta tagtcttgaa
     6481 agatacagag gactaggtca tcggatgcca atcactgcca tcgcattatc aatttccctg
     6541 ctctcacttg ctggtgtccc tccgctcgtt ggtttctgga gcaaattggt ggtgtttgga
     6601 gcggcaatta atagtggttc aatagtagca tgggctccat accttgcaat cgctggtttg
     6661 ctcaacagtg cactttctct tggctattat gcgtggatca ttaaaaaaat gtatatggag
     6721 caaggacccg atatgaccaa agttaaagag cctagggcta tagctgcggt actaatattc
     6781 gccattatat tcatggtagg tttcgggatc tggtatgcgc ctgttttaga gttcgcgtca
     6841 aaggcggtcc ctcattttgg gaatatcgct tccgtggtat ccaatagctc ctcgcccacg
     6901 tctcatacag ttaaacctgc ttctgttgct tcgtctaatt catcttctgc tgctggtctc
     6961 cgttcaattg ccgttgtgct taggtcccat actctataac tcggcttgca ggtcttctat
     7021 ggggtatgcc tagcgtgctc gaatttattc tgcttggaag aaaaatttcg ctacttaggc
     7081 gaaacagaat ctaaataatg atatgacaga atctagattt ttactctagt tacttttcat
     7141 tctttcttga cctgacacga agggagtgag ttatgtgcaa taactattca aaaccgaagt
     7201 gaagccaaat cagtcaagcg ttccaaatgc ctcttcggag tactgtcgtg tatctttcgt
     7261 aattcatttt tggcttttgt tgagtatgac aaatataggc cttccatacg ctcgacaaat
     7321 tctcgggact gaccattttt ctttaccaac aggttgaaca gtctatcttc attactccaa
     7381 tccattagat cgtcatgaat ttgataagca attccgagta aattaccgaa cgcggccata
     7441 gcatgaatct catcctccac gccccctgac aatatggccc ctatttttgc tgatgcttcg
     7501 aaaagcgagg cagttttata ttcaagaact tttatgtaat catcctcttt aattttcctg
     7561 tctttaccaa atttaatctc cattatttca ccctcactca tctttattgc agctttagaa
     7621 agttcgcccg atacacgcga gtcatccatc ttcgatccaa tatttagaat aattcctaga
     7681 acaaaatcag cagttaggat actgttgttg taaccatact tcacgtggaa ggatggttta
     7741 ccacgtcgaa tgttttcctc atctataata tcatcatgaa ttacagattc tgtatgaagg
     7801 agttctatgg cagaagcagc cataaacgca gcatcgtttt ctgtgacgtt tactaggccg
     7861 atcgcttttg tttttatact ttcagcagac aacaatagga tcaatggacg aattcgttta
     7921 ccgccatcac aggcatacct caatggagca tagaattcgg accaagagta gagatctaat
     7981 tctcgtctga gggcttcatc tatcttcaat aagtattggc taaacaactc catcaaagga
     8041 ttaacctcag tatttcttct agtcagaccc gctcttgccc gtaatttctt gaaataaaga
     8101 gaatattaat gcttttacaa aattactgaa atattgagta tatgatattt attcaggagt
     8161 aaacgaatga atgaaataat aataactatt gctccggccg ggatttgaac ccgggatctg
     8221 cgactcgaaa ggccacaatg cttgaccgga ttatgcagcc gtttatcatt atcagcttct
     8281 acaccaccgg agcacatgtg aatcttgttg caagtcacat tacataaaat cttttcttgt
     8341 tattatggga gccacagaca aatagaaaaa agggagccat cgtcgattag agagtttcaa
     8401 acctggctaa agatcaattt tatcggtttt cataccttag cagctttata ctccccttgc
     8461 caaagtaacc ataccaagtt ccctggaaaa ctaatcgaat gtcgcaccac tatatacgat
     8521 tgaataactc tattcacaag gtttttacac caccattggt aatttagttt ttgaaatgac
     8581 gggggttgta gaacgaatag atattgaaat cgataaacag agtttgctca agcacccatt
     8641 ctaccagatg tggtctgaag gtaagctgac tactgatcat cttcagggtt acattaagga
     8701 gtattttcaa ctagtaatag cggtccctga atttgttcga aatatccaag gtcttacgaa
     8761 ggactcctct ctggaaaccg agctagctca tcactttaag gaagagtctg atcatattga
     8821 accttggatg aggtttgcga tatccatggg cgtttcacgg gatgaattat taaattatgc
     8881 acctacttgc aagacaacag aagctgttct tactttaaac agcctgacaa aattgtcctt
     8941 cgatgaagcg gcagcagcga tgtatggata tgagaaggaa ttgcccaaga tcagtacatc
     9001 aaaaatcaac ggtttaaaga aatattacgg catcgaaaac agcgatgcta caaattactt
     9061 tgaaatacat gaagaagcag acataagaca tgcaagattg tggatgaata ttctaaagaa
     9121 ggtttctacg gacgatacag agtcagtgtt cgaagccggt gtcaagtcgt tgagtgcaca
     9181 aaacaaattg ctagatgcta tacaggagaa atacgtcggc cagtactgct aagccagttc
     9241 aaagctcttc cgacagacaa catactctgt ctggggctag gaggggtgta aatcgatttc
     9301 tcaaaacctt tataccttgt gctttatcgt ctttttattt acgggcccat agctcagtct
     9361 ggcagagcga ccggctcata accggtcggc caggggttca aacccccttg ggcccacttt
     9421 aaagtgggtt caaagtttta atagtaggta atatctattg ttaacaggta aatcgctacg
     9481 tttagaggca aaatctttca gcctacattg accggtaatt cctaccgcat attcatagaa
     9541 ggtctaaagt ctcccgtaac caaagcggcg tatacctatg ctctccggaa aacaccacta
     9601 ccatcaatac ttatttatga ctaacatgaa taaaaggatt aaaataaaag attacttatc
     9661 aagaatgcaa ctttatttta tgtggtttaa ctaggtaggg atattgcatc ttctggaatt
     9721 gatcttttgc gctaaggcta aggcaaagtc aatcgcgtgt tatctttgag cttatcttta
     9781 gccactttgt atctgtagaa tcaatatgga atgtagctcc cttagcaatt attagatttg
     9841 catttagaaa ccaagtattg aacagatcag cattcttact attctccgtg acaaactcca
     9901 atttgtatct atgaagcaaa aaaacattaa ctgactgctg ttagtaagca aataatattg
     9961 cagcaactag atcgatctgt ccaagaaatg gtcatttttt gcctcaagga ctaatgtagg
    10021 acattttata tctttggcta cgtctttaat aggataatcc ctcaattttt gatacgcctt
    10081 aaaaggcgaa tctactccaa atgtccacat tgccatgctt acaagacatc tcaaattggt
    10141 atcaaaatgc atcattattt ttacggctat gttaactaat tgttcgttcc ctctttcaat
    10201 ttctagcttt agaacatctg gtagtgttgt tgtaagtgct tcaaaaatac tccatattcc
    10261 atcaaacaat atgcatgcgg caatacgatg ctcaaaagct gcagctcgtg ccgccagcta
    10321 tccacccata ctcataccga taaggacaat acgattagcg tccacttcta tcttagaaag
    10381 tgcaaaattt actacaggtg tcactacttt ttcccaatcg ggtctaaagt gaatttgtta
    10441 ctctcgtatt acgcgtcctt gtcctaggcc atcgaatatc aaacaatttt atccacgaag
    10501 aagcgcagca tcaacaccgt agaagtatta ttcctcaacg attgaatcaa atcccccatg
    10561 atagattaaa gttggtcgag gaatattact atcgtctact ttgcaaaagt atccaggcaa
    10621 agttgttgtt gatccattat aaggtatttc tactgcttca aattctggtg aaaatagttt
    10681 agcagcctta ctgaaacatt ttacactgtt tctccatgtg gaaagaagac agggatcacc
    10741 tgtatctaaa cgaaggaaga actctgcaca ccgataaagt tgtgctgtat aactaactgt
    10801 agtacttaca tagataggtg acactttcaa atacccacat aggtgacact tttctgc
//
LOCUS       DAOR01000203            8203 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_36282, whole genome shotgun sequence.
ACCESSION   DAOR01000203 DAOR01000000
VERSION     DAOR01000203.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_36282"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5214..5335
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2423 a         1522 c         1476 g         2660 t
ORIGIN      
        1 tgagttgagc tacaaactag gattgattat ataaggaatt tccgaaatta tgcccaaatt
       61 tgttgctatt ggtgacattt gcaaatccgg acaactttat attcacgatt atattcacga
      121 tgtatgactt acatatgcct tctggacgag cattctttcc tcctcttctt gatttctcca
      181 gtataagatt gattgggaag atcattatgg ctttagatga tcgcagcagt agtggaggtc
      241 aatattttga ccctcggttc aagcgggtct tgtggtatat aattggtagt acgaaaggag
      301 gggctaatag ggcaagaatt ctcgaattaa ttaattctca tcccgctaat cctaaccaaa
      361 tttcatctga attaaaattg gattataaaa ctattattca tcatctttcc gttttggcag
      421 aaaatggatt gattatcact gacaataaag aatctcatgg tgcaacgtac tttctaacac
      481 cattaatgga aaagaattac aattcgttca tggagatact ggctaaaatt gggaaaaagt
      541 aaattaagca tcaacgacag cgaaatcata catgtatggt agtattggat tttatgctag
      601 ctgcagcagc agccgctctg ctcaatatag gcttgttagg tggcgttatc accacgtaca
      661 tccagaatat gcgattaata aagtcctact ttaccatagg ccttgttctg attgcttcat
      721 tgtttatagt acaaaatata gtaatcgtaa tcttttggtc taaattatat atcgcaggac
      781 cttcgataaa gaatatagtt gatgcttcgg cgccgtatct gttctctatt aatactgcac
      841 aatctgcggg tcttgcggtg ctgctttgga tcacaagaag ataatattct aatttattga
      901 tttaacattc aaaagagaag taaacgacga taacacacat cacaaagtca agcaattgtt
      961 agatccctaa attgaaagac acaaatctca tgacagattt gagtagttct ttaaatgtta
     1021 tttaactctc ttgccgtcaa tgacagatac ttatagaaat acaagaacaa gaacaacacc
     1081 ttctaaaaga aacatctcga agttgaaaat gcccccataa gcgctgccga tactttgtgt
     1141 tttgatatca ttatgtacaa actctaatga gatcatgtct gctttcttat gagccgacag
     1201 atttcctagc tttccccttc cgtaccgctt acagccgtat gcgttgattt tgtggttgat
     1261 gagggttctg gactatgatt ttttttctta aatgtaaacc attctttcct atcgccataa
     1321 tcatcctttg ctagcccttc tcctaaacct gataaaattc ctgcaggaag aggttgtgat
     1381 tttttgaaaa gattggatag gcctagtaaa gatccaatct caccatggtg tactcgcctg
     1441 ttaaaaatgt aggctttgcc tggattttgt tgcgagttcc aataggacgt cagtaatttt
     1501 gcaattgaaa gacctgtttg aaaggcttca ttattcttct caaagggatt attgtcttct
     1561 tttgtcgctg ttttcttggc cgagtttcga attttaaaca atctgaactc tcctacaaat
     1621 ataaggcaaa attagataat aagtgtttgt tagttaaacc ggcacagttc ggcttaacca
     1681 tgtatgcatc gatgaagtaa tgtttcattt ggggtgtttg caggtcagat atttattcga
     1741 gacggtgcat tctcaagatt caaggtcgaa tggatcagat catagtttat ttgtgcacaa
     1801 caatttgtta ttgcgtgttt agagatcaga cagttctaat tagtcaagaa taatgaataa
     1861 tattgctcga actggacttg atctgactcg tgaggtgaaa atcaagtgga aaaatgttaa
     1921 catcattaag accctttgct gtacacaaag gcaaaacaat attctgtgca cactgcggtt
     1981 acgtagctac tcaagaagcc ttgtttacgg tagatgagga catcaatctt atcgaaagat
     2041 actatgacat ttgtgcaagg aaagtagatt aatgcaagca tacacctgtt cagaagtact
     2101 cgtttatcga attgttattg ttagtatgtg gtagacataa tttacggtcg ttgttgaact
     2161 gatcttgttc gtgaaataca tttctctgtt agtcaacatt agcttcacgg aagctagttg
     2221 cacatcgcat ctaacatgtt ccattcttat tgttgcgttt ttgggaatgc attattaata
     2281 aacatcgctt tgttatgtct ttattgcttt tcaaatctca ttcaagaaaa aaatgaatct
     2341 tcttgaaaaa attaatggtc tcgtggcatc ggtatcgtgg gaggcaatca gcaaattata
     2401 gagagccgac agttgaagag atccaaaaac ttgtagaata tccggacaga caaatggaaa
     2461 catgtcgttc cactcagaaa cgacgggtgc agaaatagta tccgtgaaag tgcgccgacg
     2521 tgccaaattt aaaataatat cctctatgta cattctatag catgacagaa gaagcaaata
     2581 aaaggctgat tcaattccta aatgatggac gaaattggga aaggaaggct actaacatac
     2641 ccggtgcatt tctgttcaga ctccctactt ccaaaggaag acctgcatct ttagcgatag
     2701 agattaatcc aattgatacg cttggttcta tcactaagaa gagaggagta gtaataaggt
     2761 ctagtttgga attggatgaa attagcggtc tcttgagcca tccgaaagta agtgaactat
     2821 caaaaaaaat agatgcagta aatcctaaaa gagaggcgac tactcttgcc ggaaaagccg
     2881 gtcctgatat atttgaaata tgagtcagaa agtaatttct cgaatttgga atatattcgc
     2941 atataacagg ctcgtagagc ataaatctct cctagtgtaa tgaagaatat agacagcctc
     3001 taagtgtgaa tctgggaaac cgtggtgaac aggcacggag aaacgggaaa cttgaacttg
     3061 caagggggaa ggaaagatat taacaatatt aaatggcgta gaaacaaagt actggaattg
     3121 gtcagcaatc tttactattg atgttccttc tgcagcagat ctgaatatca gaaagagagg
     3181 agcagcggcg atctaggtct gttgccgtac tcttgctagg catttccaaa ggaaccggca
     3241 tctcttccaa tctctgctat gcgatgtgac ttaccaccag agtctgcgaa cgccaaatct
     3301 ggatgtgtgg aacctaacaa cctatccgta cattattcct tcccttactg attttggact
     3361 ctgtccttgt tcctgttgcc cttgttgttg ctgctcgaca tatgattgaa ttatcttttc
     3421 cattctcatt tttagacttg ctccttgttc tctaagttca tgcgtatcta ttttcaagct
     3481 ttcgttgtct gtgattctac taagtgattc tattagagtg gctgctccgt ctggatccgg
     3541 tattcctctt gctgcgtata tgagtatggc cttagaagct ataccgtttg atagacagga
     3601 ggataaaatt ccacccgacg ttcctcctac aaaagccgta gttggataaa gagtgctgct
     3661 attatctgcc atcttttctt cttctttcct tgttacattg atgctgttgt catgaataag
     3721 acttgcttca tctgcttctc caccactgct tgacaaaatt atgggcattc tttttgaatc
     3781 aggtaatcct tctacggcta tgccatccaa caccataact tccttgacct tattacgtag
     3841 agcccaatcg ataactgtgt ctaaaactga atacataccc tgaatcatta tgggtgattc
     3901 acacacaagc acacaaacgt cgcctttttc atttgaatac aggcgaaatg ggtgcctcag
     3961 ctttccttca gcataaatga caccgggtac tataaactca gattccacac aagctatttg
     4021 attcatatga agtttgtcga taatgtaact agtacttatc gatccaacca accctgcccc
     4081 tggaaatcct gcaatgagga tgggtgaatg tagttttttc tttccttgca caactttggc
     4141 caagtgttta atcctagttt tgcctcctgt agcaccatta tctatgtcag tatgagcagc
     4201 aattgccttt tttctttttt taatcgttcg tgaaggacca gatttatttc gctttggcaa
     4261 tatggaaaac tactgtcgta aaccagataa agtttgtcct gatcacttga actagcaaat
     4321 cctgcagtta ccagattttc gagcgcagcg cttgtatttc atccaatatt tcatatttga
     4381 atatgccttt gatatcacaa gaaaggatcc cgtaggaggt aactgtcaca taacgagtta
     4441 ctaaatcctt ttcagatgcc tcttattatt gcgtttgcga tattcaacgc cagttcctca
     4501 tttcaacgtc cttgttgcca gagtcttaac tgcaattgct ctaaattcgt cgtccgcgtt
     4561 gcccgacaga aagtacgttt caaatctgtc gaatagaagt tctaatattt ctctgttgtg
     4621 cagtagcaaa gactcatgca ttaaggtcca taaatattgt agaagacaag acctatcaca
     4681 caatatacaa ctattttttg tctagttgtt tgtaattctt ttgtattatt cacattaatt
     4741 caccgttatg aacggggaac tttcttgata gtaccacttg tcctatgctg cagcccatgg
     4801 cagtttgact aaaaagtatg gttgtaacgt gagcagttat agacagtggc aatttccttg
     4861 tattgctgga cttaaatgcc aattagatgt acaactcgcc ggaattttaa ggactatcct
     4921 tgaatcttta aacggataaa tcatcttgca atacaattat tttgtacgga tgatcgggat
     4981 ctaccattgt tttctcacaa acaaactctg atgagtcttt ctaacctcaa ttgaacttgg
     5041 caatatgtaa gtctatggtt cttaaaatta tgtcaaatta ttaacaagga ttattcttac
     5101 ctcatatctt tccaccatga gcctccacat attctaggag ataactaata ttaagccagt
     5161 tcctgaaaaa gaaaagctgt aaactttgaa gatagtttag gtagtattcc ctgnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagttt
     5341 aggtagtatt ccctgactgc tccgtgatga ttgtcacgtc accttatcgt cagaccttcc
     5401 aaaggtgaga tttcttatga acctattcct ccctcttctg tcatagtcta tattaacgtg
     5461 aaatggaact aagggagtaa tttttatatc atatcattcg tggttaatat ttagaagtta
     5521 tggatattat cggttcggaa aaggatgaat taaggtcaag gaacgtcttc attattgtgt
     5581 tttacttttc agccacggtt atgctttcta tttgtttctt cggcttttat ataagtgttg
     5641 gagaatatct gaagactggt aaaatagtta ttggtgaagt attagtcaat actgattttc
     5701 cgttccacgg ggatgcgact cttgtgacct atctgatgat tgcgtccgta atgtcatggt
     5761 attgtgttac caaactcgcc ggagacaaaa tgaaaaatat tccacagtca gttagaactg
     5821 ttttacaact gattttactt gctattgctg tgactgctat gtatgaattt acttacaatc
     5881 ttttcttatc gaattctttt ttaacagtca gtttagtaaa aggagtatat aacttggata
     5941 gcataagcgt accgtatcct aatccccaga caccatggaa tcttatattt gcaacaaaaa
     6001 tgactctctc tgcatttctt atttccgcac acggatttta tataatgtca aaaaatcaaa
     6061 taagggaaaa gtacaaatac actcgaaatt aacattctta ttctctgtaa taacgcagat
     6121 gccattaaag caagctcgtt ttttatatca tgtaaatatt aaattttatg attcagtact
     6181 gtagttcgaa agctgccgtc tgaaagctgt tctatacttg aaattttctc tgctgccacc
     6241 acaagttcta gcctcctcaa acctacttat tttatacaca ggtaatctta tcctttcaaa
     6301 tctaaaacct ctacattcca actaatgtgt attactagag gtccgttagt atatggctat
     6361 ctagtgattg gcctttttct ccttctatca cttaacaccc acttagttac caaggcgatt
     6421 ccaaaaattg ggaaactagc tataaacaca tatggaaaca ttgcaatgta aatggaaggc
     6481 tgaaatattt ccatagccaa cttaatattc tcgaagggca tttataattc attatatcag
     6541 aattagcgtg ttgagaataa ttaattagta tttgcttata tcctgaaaga caattgagtg
     6601 accttaacgc cttcatttca tcatgtattt aggaatacat tataagattg ctcttgtgcc
     6661 tttctttaac gaaaagcaag acctctctag atttcctaat tttataaacc agatcaaaaa
     6721 ctgcaaggga gaaatagcct tacatggaca atatcatgaa aagagaaacg gtcagattga
     6781 tgattttcat acaaggtcaa ccgcaattgt agatgtatgt atcgttcgtt gagaaaaagt
     6841 tacagctaag ttaagcaagg gttaatattt ccactctcca gatatattat tccactataa
     6901 acggtatggt ttagcatcct ttccaatttc ttcttttatt ctaaactcaa tcgaataaaa
     6961 catattctcc atagcgtcag ttcctccatc ggcgtacagt tctctcccaa tttcctctac
     7021 tctctgtttt tttgattcaa agtctgaggc atcaggattt tgtgcgcagt gagtaagaag
     7081 atcgtaaagt tcttcttcta aatacgcatc cattttctta ttactttatt ttattgcttt
     7141 tgagatagaa tataaattgt gcagcatcaa taatgacaat attattgtcc aaatgttttc
     7201 aatgtacttt caccctgtgt tgtaaggatt acattacttt gtcctccttt gtattctact
     7261 tctgccaatg ggttgtctaa ccctgctgca acactccagg actatgtaag taaaccttct
     7321 gcaaaatttc ttaccattga atcatatccg ctactgagtg tcaaacatat ttcttattta
     7381 gctctaagcg gtggcaaaat atagcttgtt tccatggtag gctgttacgc aagaatcata
     7441 taacttctgg taccaatctt ctgtatagtc ttgtcttaaa gctaattgtt tatttctttg
     7501 tttaacctca ttgaaaattt cttgtgtagg aagtctatct gactttcttg aattgcaatc
     7561 tgaacatgca ggaacaatat tgaagatttc tgtttggaaa ataaagttaa acgaagctaa
     7621 gtttatagat ttccgatcaa aaatggagag ttcgagggca aagagttaat gttgcagcat
     7681 tagatgatgc tatggacaga actagatatc tctatttcgc agtttttctt attgttttta
     7741 tattcacaca aggaggaaga tagtttgcta ctctcggatt cctttaaaca tcatttagct
     7801 accggataag gagaagtcaa gtctgccttc aattcaagat ctagttagaa gcaatcaccg
     7861 tctatcgccc gtatttcttt ggcacccacc tagttttggg tgctatcata agatctaata
     7921 acaaagaaaa tttcttgtcc gaagtgtgcc tatatggtgc cgcataaagt tccattcttt
     7981 ttaaaacttt gcagaggaaa tacaatggtg aatcaggtgt taatgctaga tcttataaag
     8041 atgaagagca taatcaattg tagaccagtg tagacacaac taaacatggt cacggtggtt
     8101 atccatgaaa aatagaaaga acaaatcaaa gcataacact ttggattcga ttatccatca
     8161 caatttttta agcctgaaca agcagttagc gagaagagat gga
//
LOCUS       DAOR01000204            4077 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_60860, whole genome shotgun sequence.
ACCESSION   DAOR01000204 DAOR01000000
VERSION     DAOR01000204.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_60860"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    346..654
                     /estimated_length=309
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1105 a          735 c          656 g         1272 t
ORIGIN      
        1 actgatattt ccaggtttcc cttttttgcc tcgctcattg cttgggtcag aacaaatatt
       61 ggtctgagga ttcttgtaat gagatacaga attaatgcaa gaatgcccat attcaagatg
      121 ccaagaatga tttctaatat cagaagatta tgggaattct tgtctacttg ttctcctagc
      181 tgtgtaacca atacatctga tgagccaata agatctaaag ctaatgagtt gaactgtttt
      241 cttattgtga atttatctat cgtatttgct ttgccctctt cgcttggtat aatcaaatta
      301 ttagagacag aagttctgta agtattccat tttgcgttaa ctgtannnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcctcc
      661 ttccttcaag cttatgatat ttgattgcaa gctattcata gcaacattta ggtgcgaaat
      721 gtcagaggat ccatctagat atctttcagt ttgcaacaaa agattggcag taagataccg
      781 attcttgcct gcaatattaa ttgagtttcc tagcgatgat tgcagagatt gaaaatacga
      841 aagtacacca aaactgccaa caattagaat aatttcaatg ataaccagta ttgtgatttt
      901 taaaacgatt ttagaaatta ttctttgtac cattgcaatt tttcttactg gtccgattta
      961 acgaaaaaat ccttttaggt aatctacttt tgttttggga caattggtga acaaatccta
     1021 atccaattct gccttgatcc gattaaccaa ataatctatt gtaactggct ttttgataaa
     1081 acatcctagg cttattgcgg gataaatttc tcttaatgct tcacgattta cttctcccga
     1141 agtcatgatg caaactctga cattgatatc tataattaac atcttttcac atagttcaaa
     1201 tccattcata tttggcaggt taatgtcaac tagtagaaga tcatagaaat gaggtttaaa
     1261 ttctgataat gctgctaaag gatcgttata tgtgtaaact tcaattcttt tgtcgtggtc
     1321 agcgtaatta ttgctacttt ctatccccat cttaaaagtc gtagtaatat cgggattgtc
     1381 atctataatc aaaattcttt tccaaaatgc ttcttccttt cttgtagtgt gaacaattcc
     1441 ttgggaatga gtttgaacat ggccgttttt agttatttgt taacacatcc ctaccttatc
     1501 tatacaagac ttactaataa atttctggat attaaattag attatcaaca tttctcagaa
     1561 aggtataata tatttaatga ctcctatcaa aaatccggga agaaattgca aatcatatat
     1621 acctacgaaa caagctaagg tcgttaaacc gatgatgaca tactaggctt tgcagaaatg
     1681 aacgaatcaa agtggtcgag ctactgatga tgtcatagtc gtctagtgcg aaacattttt
     1741 agatcttaat ttttctaacg cgtagcgtgc ctcctcttta atgcttatac attcgattgt
     1801 tacctgtgtt attccctcat tatcgacata ggtactgtat tcgcgacaat tagttgcgtc
     1861 caaaagaatg tgatcaagtt cattatgcag taaaggtgtg ttgtcagctg ttgctcctca
     1921 ttgactggca cggacataaa atacattttc cacctgtaat tttgtcggta tattcgtctt
     1981 tggtctttag gtgatactgt gaatcgtggc ggcaactaca gtttgtcatt ttgtttcctc
     2041 caatacacac agtaactttc gttcactcga atcttttctt tcagctctat tattgtaatc
     2101 atttaagatc ctcccaaatc gttgtctgta gtttggtatt acagccacat ggtcttcctc
     2161 tgtattgtgc ttcgcaagct cctgtatcgt ggttatgcat gtaactgaaa tgttgacatt
     2221 tagagcagct cctaatagtc atttaagact cccccattta gagtaacact tgcaactaca
     2281 tccgctaaca cgacaacttt cactcacgct tgatatatgt agataatagg agtgttaaat
     2341 ggttaaagaa agatgttcct aaatccgaat ttgatgttga tatcgctaga tctctcggct
     2401 acagaggtac agtttaccac ctaggaggat ccattgtggt cgataatata gttaggatat
     2461 taaaaaactt gggtgtttct gaaattgacc tggaagctga cgatgcagat tacacttcga
     2521 aatttcaacc aatgaaaaca aaagttccac ttataattga cgatctggcc acagctacct
     2581 atcttcctat aaaaacatta agagagatcg atgcacttct tttagagaaa aggcaggtga
     2641 ttttttatgg gcctccagga acaagcaaaa catatatcgc aaggagattc tctgaatact
     2701 tcactcagaa tattgatgat gtggaaatca tccaattcca tcaatcctat agttacgagg
     2761 actttgttga agggataaaa cccgctatat caaaaacttt aggcatcgag ttctcaaagc
     2821 agccaggttt attcaaaaat cttgttaaaa aatgtataga gaaccatgaa aagagatttg
     2881 ttctcatcat agatgaagtt aacagaggca atatttccag gctacaaggt acatctattg
     2941 ttatattacg atcggcattt gagcgtgagc agggctatgg cagctttacc aaggagctaa
     3001 tcattcactg ctgctgaaat gtttctcttt gatggaactc tagattagat tggtctacct
     3061 ttacagcctt tggtggacat accgtaacac aagccataca ccaaatacac tcatgctctc
     3121 taattggatc agacttgtca gtgctgtcta gcctctcttc tttaccagta ctaccatttc
     3181 cgtaacttgt ggcattaacc atatctaccg ctggtacatc attttctgtc ctataccatt
     3241 gaaatacact taccgggcat gcttgtatac aagctccatc agcaatacac gaatcccaat
     3301 caactgcaac catagtgcca tgtacaccga cagggacaag ctcctcattt ctagcagcat
     3361 aagctgctct tacctctcca ctagatgagg catcagaagg acttccggga ccccagacaa
     3421 agtgatagtg tttgccatcg gcatgattat gtctaccaaa cacttggtgg tttcttggga
     3481 actctggatc tataggcatg attaggtttc gaaaccaata ttattaaggt ttgaggttta
     3541 gttgtaatta tttccgtaga aatattggtt aatgtaatca gctggagagc tctctctatg
     3601 agctttcttt ccagactcgt ttcatttatt ctactgttac tttttcaata acacgcttgc
     3661 tttttaagtt gactttttcc aaactattag ttatcaactt tactagccac agtattgcgg
     3721 caataacata ttgcgtccac tctgaatgtc ttctcttctc ttcttcttct ttcagataaa
     3781 gacggtatcg cgttgctgca atcctgccta cataatcgga caaactattc aatatgggct
     3841 tgattgaata taagaactag ttgtgaattg tatttttgaa ttagtgaatt attatactga
     3901 aagtatttaa taagtataat ctgggtgata acttcttgac atccaagcct gtcagacaac
     3961 tgagacagat tcttcttgtt gccctaccct cccactctct gtaagtagct ctcatatttt
     4021 tagcagtcca accttttctt tacggtcact atggtttgta gaaatctatg tgagagg
//
LOCUS       DAOR01000205            6916 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_36324, whole genome shotgun sequence.
ACCESSION   DAOR01000205 DAOR01000000
VERSION     DAOR01000205.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_36324"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1889 a         1507 c         1381 g         2139 t
ORIGIN      
        1 attggcgaaa catggtaata aggggagaag aagtaatcat ggagctgatg ggaaaaacca
       61 ttaattatga agtaaaagcc tatggctctg aagtcgttaa gaacctgaag tccgtcaacc
      121 cgtgaatgtt accagcatgc tctcaaaccg tgataagtaa ccagtactct tattactcac
      181 gtttaaatag gacatccgcg acctaaatta ggttattatc aattgacttg taagggtata
      241 tgtgtacgtc ataaagcgca gaaacctgtt ggttctgggc gttatgcaaa cggacaaaaa
      301 cgatgtcaga tttgcgagat tttcatgaaa tgggacggat tatggtgccc gtgttgcgga
      361 tatagattaa gaacaaggcc acgtaaccta aagtacaagg ctaaattgcg gacaaggaaa
      421 aacgaaacga tggatagtgt tcaagcggca gtaattctac ggtagttttt cagcacattt
      481 ttggctgttc tgaatagctc aagctgcagc ttttgaatct gcggcgcatc aatactcaga
      541 tccatcgaca cctgtattag tttagaatat gagttgcaaa aggttgcatt tttatttacc
      601 aaaactttcc gagctatttt gccgagatag taataacagt ctggcaacat gccaagcttg
      661 gttagatgtc ttgctttctc gataaccaat tctttatcgt aatcttcgga atatagctgc
      721 agaagggccg aggatgatct cgatatcgtg gatctggttg ctctttcgat gcctatgcac
      781 gcaagagcga ccttgatccc attggagata ttttgttcct cagaatttaa atttcggatc
      841 tgatttaatt catgaagagg cattggcctg taccctgaca gggcaagtat cccttctaag
      901 aaatcgtagg cggatatttt aagccacatc gacgcgagaa tagggctttt tgacacaaca
      961 ctgtcgatct tgtcgtagca aaagagtgaa cctacaatag ctttttttcc gaatgcagta
     1021 agggcatttg catagtttga ataatcgaaa gattggtggg acaagccgtc gggacttgat
     1081 gccacaaagg gatagtctgc cacaaaatcc ttagctaata tcataccgtt cgtggctatt
     1141 atattttttc tgggattagc agatatgtta attaattcaa tattgtgatc gcctatcctt
     1201 aaaaatttgt tctcgtgtcc aggagcacgt tcactgaaaa ttgctagatc gtattcacaa
     1261 caatcgagcg aggttttagg agctgttgcc ctgcaaccaa taagggctac aggatacgca
     1321 atattattgt caatatactt cttgattgaa atgggaattt tctgcatagg tgctttgacc
     1381 tgctcgaacc tttatgagaa agatcgtgag taagaattta caaagcgggt agattagatt
     1441 agattagatt agattttgat aaagatggtg cacacaagtt ttatatgaag agcaaagaga
     1501 attggtgcta ctccacgaaa aatcggcgct ccggtggtgt agtccggtta agcatttcgc
     1561 cctctcaagg cgacgatccc gggttcaaat cccggccgga gcatgtaaga accactacag
     1621 aactcggtag tccggtattc ctcttcatct ttgtctgttg gcatctttgt cagactaata
     1681 ggtggactgt cgtaatcgtc atcattctgc tctagtcgta gagaggggtg cgtaataaag
     1741 attcccgcaa tgaccatctg ctctcggccc gtgcgcaatc atgccaaggg aaggtcacag
     1801 ccgctatggt tggagacgaa gccacttact tacaaataag gatattatcc ctcgccgcca
     1861 caacagacaa ataggaaaac ccccactctt aacgagactt gccatgcaat acgcactcat
     1921 attcttcttc gattgctttc aaaaggatat ctccatttat tcttccctct gattttagca
     1981 ttgcagcaat tgaagtacct ccagctccca caccgtcttt tacaaatcct cttgcaaagg
     2041 cttctaagcc tcggatatgc gacttttcca tacgcagatc agctgcaaag gctggaactt
     2101 cggtcgagat tgaactgaca agtccagcga gatttgaaga attatcgttg gctacatata
     2161 tggtagtccc aatacatact tccttcatct tcaggccaag cgatttcaaa attgccaaga
     2221 ctgctgtcat ctgggttcct ccagcgagga gaactttccc tccgaccgat agtacccctt
     2281 ccgatatacc tgcgacagat ggcatcattg gatcacctaa aaaagctacg gcttccaggg
     2341 gattatttct taattcgcca aatgagatcc ttgacttttg tatactgtgc ttgactatgg
     2401 tattttttag acgatgagga tttgtctgca tgctgctact caccttatac tttgcatcaa
     2461 taccaagcgc atgaagaact cccagcgtcg tagtggtgcc accaggaatg ctttcgccta
     2521 agaccattag gtcgcaagat ttacctaatt gcctccctaa tatacgtccg tactcgaacg
     2581 ccttcttaac atcaatagat tcaactgcca caccggattc gatgttgtgg ccagggtcta
     2641 tcccaaagga aacataggga atggagggtt tgatctttga accagcgtca actaccagga
     2701 agggaatatc tgctaatcca agggcacctc ttgttataat agccggagta ggatttccgt
     2761 caggcgtgat cggcacttta tcgatacatt tgcaaaagcc ataatacaaa aattcagcat
     2821 cggcggcagg ggtgtatttc acaaagtctc gattggcgcc tgctgcggtg atgccgggaa
     2881 tggcactagt atctgtatac gatatcacgc agatgaataa cggtgacttg gttgaaaatc
     2941 tggttatcga agaattgccc tgatttatcg ttcttatatc gggatactga ttcctcgaca
     3001 agactcactc atcagcactt gccataaaac gcagaaaatc tcctttggat ctcgctttca
     3061 gtacatagtt ggtttgcttt tcctgaccaa tcgtggaagt ttgtgttggt ccataattat
     3121 gcccagggta gacggtcatg gaatcatcta aatttgctat tttgccgaat aaactatcat
     3181 acatctctga tgcatcactg ccaggcaggt cgattctgcc gcaattcccg ataaatagag
     3241 tatcgccagt aaatactaac tcattgttaa ccacaagaga aatactatcc tttgaatgtc
     3301 cgggcgtgtg aatgatcctt atcatgatct ttccgatcga aattatttgt ccatcttcta
     3361 caggaatgtc attgggttga gtcgagtttt tgtgctgtat aatttttgcc tctgttactg
     3421 cagcaatttg ttgattgccc agaatgtgat cgaaatgggt atgagtatta ataatgtatt
     3481 ttaccttcca gccatttttt tttacgacgt caaaaatttt ttccaaatcc caagatggat
     3541 ctattaccgc ggcttcgccg ttatcttcgt cagctaacat ataagtgaaa ttatccattt
     3601 gacctaccgg aatttgtatt atttttaccg ttgtaaagtc acctcaggat tcctctctct
     3661 gcttccgtcg gaattcctat atgctcaaca taaacaatgc ctgtatgttt tctatcgttt
     3721 agaagaccac tcttgagcct atgaaaagtt acggtagcat cagccatgat acatttttgg
     3781 ccgcttttgc cagtatcggg atctagtcct gaagggatat cggccgcaac aacccatgca
     3841 tctgatttgt ttattaactg tattgcagac gcgtgtggtt catgaatatc gcccctgatg
     3901 cctgttccaa atatgccatc aaggatgaca tgtgctttgg aaatttttgt tctcaccttg
     3961 acatcgaacc gattacaaaa actggtctct atggaatcca tttttttcag gatctcccag
     4021 tttatccttg cctcttcagt tcgcaaatca cccggtcgcc ccaataatat cacagtaatc
     4081 gcataaccgg gatatccgct gagatgtcta gctgctacaa acacgtctcc accattgttg
     4141 ccggtgccac agaccgcaac aaggcgcgtc atccgtggag accttgtttt aaagttagcc
     4201 ctcacaaaat cagcaatgcc gtgccccgcg ttctccatca tatatatacg atgcattcca
     4261 agacgactat gagctttttc ttcgatctga tacatctgag ttgatgtgat cgccttatcc
     4321 agatgcctaa tcgtcgtagt tcgtctcaat atcaaattgt gacctttacg taattgtaat
     4381 ttcaggagta tttataatat tgtcaatgaa attgcatggt gccgctcatt ttctcgtcaa
     4441 tagcagcgtt caatcaagta gtgtcaggcg tcctggcaga aaaaaaacac ttagtatggc
     4501 agatgaacat ttacctttac attacgtaga acggatgttt tatgctctga cttttatcaa
     4561 tttgacgtgt ccacgtgtgc aattctcagg accgcctaag tgtgaacgca aatgctccct
     4621 tgcttttgac tcggctagct tttccgctga tatgtagcca cacttcttac atgtcacaat
     4681 atagctccat ctgaccaaac tggctgtacg ctacttgacg acgggttcat tttatctatt
     4741 tctaaagttc tcgttgcttc ttgtttcaag ctttttttgt tgtcctacct cgaaacaaca
     4801 gaatcttgat tatctggtgg gtcgatttct tgattgaaac atgtataggg ttattgtaat
     4861 gctaggagat tcattggtcg gttaagcaga gttgtcacgc agaaatgata ataatccaca
     4921 gcctttcatt tgtttatttt agcgaagtga tcccaataca ggcttaaata ttttgggtct
     4981 cttctgaagc tgtcttttcc gtgttgcgac ccatcatact aattcgcaag tcgaagggaa
     5041 atcatccggc tgtttatgac agtgatacac taatgggcta atgggacttc tgagacccac
     5101 gtttcttacg ttcaatgagg cccatcagct gataaccata ttctaagttg tgcctaacat
     5161 gattaattcc atgtgagcca tgtggtacaa atttaccatc caaaacgttt tcgatgtaat
     5221 ctctcgattt tctaactgat acacttgcat gttgaagctg gttatacgta agtcttctac
     5281 ctagctcatt ttctacctct tttttaaatt ctccctcaga agatattaag ccactgtact
     5341 tttgcaattt gttgtaattt aggcacagct cagttagctt cccgacgtca gcgctctcaa
     5401 ccttaccaag tgattctatt aagtctgacc ttttgcgtcc tgttatttga cttaaactct
     5461 cgataagatc gtaatctttt tcaacaatgt ctatatgttt gctcgaatct cttttggggg
     5521 gccatacctc tgattttctt gcctggtagt atctctccat actatgggcg cttacctccc
     5581 taccaactgt atcccttcca tccataagaa ctgaaaccat gtcaacgatg attttcgggt
     5641 aatttgcttt caaagattta tcggcgcttt ggtagttttc cataatatct ctgtagatct
     5701 taaagacaat gtcgcttgat gcgaatactt tgccatgttt tttatccatt tgtttggtag
     5761 ccaacgtaag aaaatcgtat aactcagatt gactgaatac gtgccgatcg ttttcaccac
     5821 agatgtctgc gatggtcttc gtgacggtca gccatttgcc accactgata ccattttgtg
     5881 ggagtcgttt aactgccaac tctgcaatct cactcccaac ttctgtctcc actttcacct
     5941 taaggatatc taaggagtat tttagagcat tcgtcaaagc agtataacta ttgtcctctc
     6001 catctaacac cttgctcgct gtgattggac tcttgccact agtcttggcc ctcatatttt
     6061 tcctcaactg atttgtatgt taaattcctg agcgatacga tactagaagc tgcaagcgaa
     6121 ttcagtcgat ctctagggta ttcacccatt ctgtcattag tactctctgt aagcttatta
     6181 acgttactaa tgtttggtaa tgatcactat ctttttgctg taagatgaaa gtttctaaat
     6241 ttacaagcaa aagatatcga aaaataatta caagatattt cccacccctt ttctaataga
     6301 agaaacgaag ttagacctgt ctttacagcc tgccaggaga ttaccttcgg ctggggccag
     6361 ccatgcttcg agaagatgct ctgcaaactc atagtaatta agatcctacg tttcttgctg
     6421 aatggtccct atcgtttgta gctcgctttt ttctgaggct tgtgctttct cagaaggaat
     6481 tgaaagcgat aaacctcact atatttgtgg gacccatact gattgcagta ggaataatca
     6541 tcgcccgcaa cattagtgaa caaaatagat cattaccaga acggttgtta tggagcacgg
     6601 tgtccttgtc tctaaactga cgatactgcg tcataccaat gttattgccc gtagaatcta
     6661 gactctcgta aaataatata tgatgtcctt acaaaaaaat cactattaat attaataatg
     6721 atagaataaa ataacgaatt atgttattgt agtatattgg gatttacaaa ccgtttttcc
     6781 actcttctta agcagaaggt gaacacgcta ctagacaagt acgaagaccc taaagaggca
     6841 ttagattatt catacataca acaggtcgaa actctaaaca ggttacgcag gaatattgct
     6901 gaagtggtaa cagcca
//
LOCUS       DAOR01000206            2634 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_51398, whole genome shotgun sequence.
ACCESSION   DAOR01000206 DAOR01000000
VERSION     DAOR01000206.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_51398"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          750 a          561 c          513 g          810 t
ORIGIN      
        1 gatgaagaga tcgatgcact attagaaaaa tatggattag gcacaaacga aattacacgc
       61 gctgttatcg aatgcagagg taagagcagg agatgaaaat atttcttgat tctgctaatc
      121 tacattccat aaaaaaatac aatgatatgg ggctcattga tgggatcacc actaatccga
      181 ctctactgtc gaaagagaaa gaggatccgg ctgccctgat gcgggaaatt gttagaatag
      241 taaagggtcc tgtaagtctg gaggttgttg gtactaaaac agaagaaatg gtagaagaag
      301 ctcatcggct aatgaagtac ggccaaaacg ttgttgttaa aattccaatg atgcctgatg
      361 gtttaagagc cattagacag ttaactcagg agggtatccc tactaacgtg acacttattt
      421 tctctgccaa ccaagcaatt ttggcagcaa aggcgggtgc atcttacgtt agccctttta
      481 tcggaaggtt ggacgacggt gggcaggacg gcacagacct tataaagcaa attgttcaga
      541 tctttagaaa ttatcacttt aacacagaag tcctcgtagc tagcgtaagg catccactcc
      601 atattatcga agcaggaaag attggagcgg acatcgtaac gctcccgccg gatatcttgg
      661 caagaatgat aatccatccg ctaacagata agggcttggc tgcttttctt gaggattggg
      721 aaaaattaaa aaaggagaac cccaacgtcg gaatttaatt taatcgcgaa gcaaatatat
      781 cacttttttt ggtatgtctt ccaatctgct gaccgctaag cttctctcat aggtcaaata
      841 ttttagatta tggcctatac ttattgcatc gtttaagttc ttgcctaatc caagtgctac
      901 catatgtata cccattttcc ggtatgaaag gaccatttca cggactgcat catagtcgga
      961 tggctctcca tcagtcaaag tcaccatgat atctggctta aatgattttg caataggctg
     1021 taataatcca tatatctctg caagcggtgt gccgccgctt gcccgtatct gggtaagtct
     1081 acgcggccct gttgcagacc acttgaggtt tggaggcttt attatccaac acttcacctg
     1141 tcctgattcc gtactaaaag cgtaaacagc aaattttatt cccaaatagc tcagtgcttc
     1201 gcatagagca actgttgctc gtttgtactc taattccacg tgctctatac tgctagaatg
     1261 gtcaagcagg attgcaactt ttgttttgat ggagagttta aggtcagtca aaaaattctt
     1321 aggtaacatt tccacatagc tctcaggatc aaattcttcc cctgtcactt cgtgtcgttc
     1381 tagccaacct gttctccagt ccctgaattt agccttcacc tttcggatta aatcagcatc
     1441 atatattggg gatggatcaa catccatcat ttcaggaacg cttaaaccga ggttttccag
     1501 actctcataa cccttgtttt cagttttctt actctctttg agcagggttt gaaattcacc
     1561 cagcacgtca cttccttcaa taatttctct ggaggttctg tctaaatcag tagccttaat
     1621 attccttcta acaatttttt ctatttgttt cacaagatcg ctctggttca tccctaaacc
     1681 tattattctt gtccgaggcg caaggatagc tacggacgtt aatgcatcta attcaagcat
     1741 tttaatcaaa aatggtatat gtctttcaat ccactcagta tgatagttat tcttgattgc
     1801 atcatggact atttcgttcg caaaagacga tgccttcttt actttctcca aatcgtttcc
     1861 gtacaactcg cctttaagat aacctgtaag gaagtactgg gaaaaagctt caataatctt
     1921 tgactttcca taaatagaat taaggagcgg tcgtgacaac caagacatgc cttcgttaaa
     1981 tacgatctct ttaatcattc cttcccaatt ctgtagtcct aggatttcaa ctcgtttggt
     2041 ttcaatagta ttcaggagaa atccgaaagc gtgttcataa ctcaagactt tggttgaatg
     2101 gtgcatacgc atagattcat gccacaatcc gactcgccat tgcctgtatt gctggaaact
     2161 agtgccgaga taataattga gaaagggtaa tgtaatctga ttcttatcaa tttttgctaa
     2221 aggcatttta tcttgagtta aaattataac ggtcttgtta ttgttagacc atcgtctact
     2281 caagaaggtt gcaatatcaa gcaagacatc gttccgcaga tgtgtgtagt taagccctaa
     2341 tggcaccgat ggtgttggca taccctagtc aacaaaaatt gatgttatca tatccattac
     2401 cttttgatat tcaacttcgc cccactgctc ataaacatta gcataaacaa tctctgcggc
     2461 ctgtcttgca ttgcttccgg cgttaaggaa tttcgcaaaa gcgattgact ccctcaaact
     2521 aggactatag tataattctt caacagccgc tgcttcgcgc aaattctttg caagtttgat
     2581 cgcacgtttt atgtcattaa tcttagatat atcgactgtc gtatggcgtt gtac
//
LOCUS       DAOR01000207            4871 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_68038, whole genome shotgun sequence.
ACCESSION   DAOR01000207 DAOR01000000
VERSION     DAOR01000207.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_68038"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1637 a          898 c          935 g         1401 t
ORIGIN      
        1 tatgtaagta ctacagctaa tgatttggat agagaagaga ctgggagttt gttagagtat
       61 tgtgtttcac tatgtagtgg actattgccc atctaccttc ttctagtacc gtttactatg
      121 taacaccatc acaattgtct gcagtttgtc tgccagtata tcataataat ttttttccgc
      181 ctccaaaaat tttctatacc ttgcagtcat gattctgaac cttggaagtg ctacggaatg
      241 ggttttctgg ttgtaatttt atgtgtaatt tccctagtct tggctaccgc atctccgttc
      301 ggtactctgg ccgtaaaagt accaccgtct aacactcatt tttcaccgat aattccgtat
      361 ttcatgcctc cgcaaaatac ctcaggtcca gcagcactac aaggcccagc agcactacaa
      421 ggcccagtag cagcactaca aacacctact aatgccacac taccaccact acaggctaat
      481 gccactagta agacttcaaa ccaaaccggt acaggaggct ttttgtcaaa gggaacaata
      541 aattcactta tacagacacc taccggaaat tggatagcta ccggaaattg gagtatgagc
      601 gtagataatg gcagcctaac aggttttggg actaatatga cttggtataa tagtaatggc
      661 aaagcgtcac atacgcatga attccgtaat cttagggctg gagtattaca aggtaacaat
      721 acaattttga atggtttgat ggatgcggga acaaaccacc ggatagtatg gaagaatgta
      781 ccaaccacga ttagtattca aggaggcaag acgataagaa tatcggtcgc cgataatgcc
      841 acaaaccatc attttgcagg acaacccgtc tttggattgg tcacctcatt cataccgtgt
      901 tctgatgttc caggccctaa tatggaagtg ttaccaagct gtcaaaagta gattgtagaa
      961 aatttattga tgatagacgc tggcaaaagc ctaaagacta tgatgataag tgctcctaac
     1021 aaaggacaca caatagataa aaaaacacag atatctgcgt ctggatgcaa tgtctacgta
     1081 cttgagcctg gactacgctt tcgtctgacc gatagatgca acgtggtctt cgtggctggt
     1141 aaccgatgaa gacagtttgc aattaatttt cgtctgctta catacaaacc agtgtttaca
     1201 tttgttgcag tagtgaatat ggtcgaaatc ataatcccaa gttgactttg gatttagtag
     1261 tctaaactat tagcgcctcc tcaacgctca ttgcttagtg tagggtgtca gtagctatta
     1321 actcttcaat cacgccattc tcaaagcttt ggacagtgag ctactagtag gattatgaaa
     1381 ttatgtagtt atgctttcga aatcaaacaa ataaaattag aggcttgaat gttctcgcta
     1441 ccatgccatg atatcattat gtttttaatg gttttatgaa tcatatataa gattcttatc
     1501 gttaagatat tctaaattcc tttatgatct tttttagata attgttagat gatggtatta
     1561 atgctaagaa aatccaaaca gataagcgtt aaggagcagg agaatcaggg gcgtcaactg
     1621 ttttcttcta gcgaacgacc atatgaggga caaattggct tcgatcgatg ctgtattcaa
     1681 gtcactatca gaccataaga ctcttgtttt gtttgacacc attgctcatt cagattcaga
     1741 tatggatcct ccaataagta agttgggatt tacaaaaaag caattctatt caagaattca
     1801 tagtctaacg aattctggtt tgataacacg acagaaggga aagtattacc ttacttcgtt
     1861 tggaaggata gttttctttt accaatctat aattgctaga gcacttgaca atttctggaa
     1921 gctaaaggca cttgattcta tatctaagga atctggcaat gatctgagac tcatggagtc
     1981 ttatccaaat ctagttagta gtttaataga agatgtcgaa atcagagaaa ttcttagaaa
     2041 cgttcatagt ccctctctaa gcgtaaaatc tcacatctta cgtgaagcag ctacgcaggc
     2101 aaatcgctga tccatgttct gatgcctttc cagaccttta tcgttatcat aatcctgact
     2161 ttgacaactg cggtaatatt attctcccct aataccgtgg gtgctcagga cgctccacca
     2221 atacaggagg tgggcatcat caagcacaaa acttctcctc cttctcacgt tatatcctca
     2281 aatgagagca atacaattcc atcatcatct tcttcttctc ctcctcaacc aaacactgcc
     2341 tctcctagtg gttgcatcaa ttataatcct tccacaagaa caattattgt cagctgtagt
     2401 agtccagcta gactttcaga tattgataac aaactccatg atagcagtat cctagctaaa
     2461 caatctacaa atggcgtatg gtttctaaat gcaaatctag taattgccaa aggagctaca
     2521 ttacatattg attctacaga tactaaatgg ctaaagataa gctcaaaggt aacacatgct
     2581 ggtattgcta agatagctcc tgcatacatc atagatgtac atggtagtct aaagattgat
     2641 tcagtaaaga taacttcatg ggatccaaca acaaactact atgcgataac taatggctca
     2701 aggacagaaa gtgatgtatt tatttttggt gctcctaggc catatattgt agtggaaaat
     2761 aatgcaacag gaactacgga tatcaccaac tcagagattg catatttagg ctatgaacaa
     2821 ggtaagcata gaggtggaac aggactgagc tattattacg gaggtgatgg aagtatccta
     2881 agaaatgaca atatacacca tgtttatttt ggcctctata cttttggtgt aggtcatatg
     2941 attattgaga acaatattat ccgtaatagc ggcctctacg gtcttgaccc acatacaaga
     3001 acacatgata tgataatcag aaataataca gtttatgaca ataagggaat tggtataatt
     3061 tgttcgttaa attgttacaa catcgtcatt gaaaataaca aagttcatga taatgcagct
     3121 tcgggaataa tgtttagtcg aaatatgact agctctattg caagaaacaa tatcgtctat
     3181 aatgaaccaa aaggtatttt tgtttcacaa tcacataata atcagatata taacaatact
     3241 atttcatata gtggaaatgg aattaatgta tatgctggtt ctaccaataa taaaatgtac
     3301 gataatacaa ttatgaattc caaatctcat gccatacttg tcaataatgg ttcaaatgga
     3361 aatacattct attcgaataa aatagttagt gccatcaaag agggactcga gattaagcaa
     3421 gatgcaacat caacgaataa tgtattctcg aataatcaag taattaattc agcaggatca
     3481 aataatacta ttaccgatga aataaataaa aagaacaatt ccgagatcgg cggcagggac
     3541 cattaggtcc acggtgttgt agtagtgaat gattaacagt tatgcaacga gactatcagc
     3601 agtggttttg gataagcgcc ccttatgtta tttttttcta gcaagtggta tatgcaattt
     3661 agtcgatctt ttaattccat gatagctttc ctaagagact atattaaaaa acaggatgat
     3721 ttaacaaagc agcttgaagc agcaaacgaa gaactcaaac gcaaagatca attaaaagat
     3781 gaattcatta atattgcagc tcacgagttg cgcggcccta tgcagccaat attgggccta
     3841 gcagaggttc ttcggcatag aaaaagagat caaggagaca gtattaatag tagtagcgtc
     3901 agtaaacaag aagacaacaa actccttgac attatcatta gaaatgcaaa gaggttgctg
     3961 cgactcgaac agaacatctt ggatgtggca agaatcgaaa gccagtctct aaggctagag
     4021 aaagaaagat ttaacgtaaa tgaaaaaata cagaatgtga ttaatgattt tgggaatgag
     4081 ttatcgaatg agaaaataga gcttgtgttc acaccgtcac aaaaagagcc aatcttcgtt
     4141 aacgcagaca aagttagaat atttgaagtt atctcaaatc tattaagtaa tgctgttaaa
     4201 ttcaccaagg atggaaagat tacgatcaac gcagaaaaga aagacaccca agttcttgtg
     4261 agtatcaaag atactggccc aggaatacaa ccagagctta tgccaaagct attttcaaaa
     4321 tatgttacaa attcacctgt aggaacagga gtaggattgt ttatttctaa aagcatcata
     4381 gtagctcatg gtggcaaaat ctgggctgag aataacgttg atgcaaaggg tgctacattt
     4441 acgtttaggc tgcctctgga atgttgatac aaaaacaagg agcagaagaa cacaaagctg
     4501 caactaatga agaagaatat aagaagcaat ctctcgggag aacgagaatt ctaatcatag
     4561 acgatgaacc cgatattact ttagccttta taaaagctct aaaggataag ggattcgaac
     4621 gggttgagac agttaataat cctatattag ctttgaagaa tttcaaggca ggttctcatg
     4681 atctgctaat tattgacatt gtaatgccag aaatggatgg attcagccta tacgaagaaa
     4741 ttagaaaaat agacaacaaa gttaaggttt gttttatcac cgcatttgag ataaattatc
     4801 aagctctgag agctgtattt cctgctgcca ctaccactga tgatattgga tgcttcatcc
     4861 gaaaaccagt t
//
LOCUS       DAOR01000208            6714 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_5684, whole genome shotgun sequence.
ACCESSION   DAOR01000208 DAOR01000000
VERSION     DAOR01000208.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_5684"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2018 a         1339 c         1234 g         2123 t
ORIGIN      
        1 aagctaaaca agaccattga cacaacatgt atttctaatc caatatttcc ttgaaggaca
       61 cgaaatacaa tgattccatt atcctcaaga ttaccaacta tctctcccaa aatccatacg
      121 actagaaata tcgagatctg aaattgaaaa cttctgatac ttttagatcg agatacttgc
      181 catacaaata tggccaaagc tatcaaaagc agaattattg tgatgttgtt cataaaaaga
      241 aggaagatac caggattcaa cgccataacc atgttttttg tacccaaatt ctttggtttg
      301 gtgaaaccag aatagactat gccaaagaat attaatgatg taacagaaag cggttgaaat
      361 acaccttccg ctaatttact atatccaaga aatccaaata catgatacac agcatgtatt
      421 agtatgaaaa ccgtgagtat tagtgtcaat ttctttagat tgtctttcaa ttttatggtg
      481 aggtaaatag gaactgctga agaagcaaga ataataatgg cactgataaa ttgaagttga
      541 atctctatca ttttttaaaa tacctcagaa tttctagata aaagaaatca ttgctccgat
      601 aattaatgcc aaaaatgtag tatctaccac tgttaaatat atattgcatc taatcacaag
      661 ttatatcctt ccagacaaca ccgtctctaa ttatatgcat tcataataac aattgcaaat
      721 cttccatggc gtaatctcaa acttagtgaa aagaaagagg ataagctcaa gtaacatatt
      781 gtagaaaagt aaaacaattg gcggattgtt tttggttgat cactgctgct gaatgaaatg
      841 ttattttgtt aatgcacttt cttaatctta gtatttggga cctgttgtgg gtgctggaga
      901 tgatgtactt ttaggcattg cagaagatga ggacgtggtg ttaccggcag tcggattacc
      961 accaggagca gtaccctgca cttgtattat tcctttcatt gtatctggat gataaaggca
     1021 atgatatcca aatgttccaa tcatgtttgg cttaaatgat acttgacctg aactgccagg
     1081 tgctatatct ccgctagttg caagctgttt accgtttagg tctataatta attgatgttt
     1141 ggtgtctgtt ggattttgaa tttttatagt ttggttagtg tttactttga agttcagggt
     1201 gggattaatt gctccgttag tactgctgac ccacttgaaa tctgacgata ctaacttagc
     1261 ggataattga attgcgttac tgactattcc ttggccagat acccctctat ttgtagtata
     1321 cgtagatact aaacccacta caagtaaggc taaaagtcct actattagta ctggggcggt
     1381 tgctcgggta aaagtttttt gcatgagtgg ccataaatca ctttatcatt attataaaga
     1441 tagagacaaa taccatctct gggacccaca aatggaattc gtatctatat tcttaaggtt
     1501 aacggaatta ttactctcca atgaatgacc ctaaagtaga tagaagtaat gacaccccga
     1561 agaaaaaaac catcgctgca ttagttgttt ttggaatgat aattgccttc ccgcttgttg
     1621 ttacagacaa gctcgctttt gccattggca cgaacagtac gacaagtgga acagcaggag
     1681 cctcgagatg aaactgagta caacaagtgc acctgtagtt attccactag ttaaggcatt
     1741 gtatgatagc aaaggtgtgc tgttcataat aacagaggta tcagataaaa caatgagaga
     1801 tcaaattgga aactttacag gatccctagt aaactatgaa cccaatttga caaagtcaca
     1861 agacatagga caactatgga tattcaagaa cggtgtcaaa ggtcctggac tgatgggctt
     1921 tcaaacaaat gtcttaaatt caattcctgg agatccacac tatactcttc tgtggaaagt
     1981 taatatcgta gaatggaaga ctacaggcac aaacgccact acaccaacta ttcttggttc
     2041 tgatgatatt attgcaaatg cagttaaaaa aggtcagata actgttactc ctacaaatgt
     2101 agttattaac tgtcctgtgg ttcaatgggg aggtaacaaa atcaatacaa tccctgctgg
     2161 tcatatatga agcatcactg aaaagataga cgatttttcg gctacatgtc tcccataaat
     2221 tttttgtgtg gttcgaaact tatgtctgct acagatcaga atcaaaaacg acacaatcaa
     2281 caataccaag atatgctatc ttgcttcaat aagatattgg taattggatt agggcagttg
     2341 ggattgcctg tcattaaata tgtaaaagaa agagattccg taaaagctag cacagagatc
     2401 ctcagataag ctattgccga ttccttacac atttgaccaa gggttcttgt atgttcaaat
     2461 ttggcatctt ctatgactgc atacaaatag ccgctacgac tagcttcgta cagaagtgca
     2521 gactcgtaac ccatctgttc tggatagtaa ccatgccaga tacttttttc aaaaaatgaa
     2581 tttctgacaa atctaccagc tccatgtggg gaaaaaaact tatgattagc ataatttcct
     2641 gaggcaacag cgaccaatgg atttgaatcc atgtacgaga tcattttttg ggcataatct
     2701 cgtgggtaga aggtatcatc gacctagtgg aactggtact ggcagcaatc ggacacacga
     2761 cggtccatac ggtcctaaaa ctggtactgg cagcaatcgg acacacgacg gtccatacgg
     2821 tcctaaaaca ggaggtaaag gagcaggaga tcaaaatgga gcaggacctt gtgtagatag
     2881 tcatttcggc gcatatggaa gaggttgcga agtagggaca cattgcatat tcatacaggg
     2941 acatggtctg ttttgtagac atgggtacgt aggaggagat agtcacaaca tcaaacacga
     3001 gggtggcaca aaggtgatgc acgagacaga gataagggta attcgagaac ctagcagcgt
     3061 tccatataat ggccccagtt cagtagtatt gttatcccct acatcaaacg actttacgaa
     3121 taacgacccc accacgattt cagtagacca tgttaccatt acaagcaacg gcgtgcaggg
     3181 atggataaaa gggacggtga cgaacaactc gaaccagacg atgcacggat taaggataac
     3241 gggaacctgg tacgactcgg gcaatagcac cataggaatg tcaagcggtt acgtaggtgg
     3301 actcagcctg aatcctggac agagtggaac atttagtcag tttgcaaact atgacattaa
     3361 cagaacacca agtactgtgg agttgagtta tgattggcaa tgagaaatga acacaggata
     3421 cggaaacgta ccgaatccgt ctggacaact tagcaattac gtaagagaaa cttaaaaccg
     3481 aaaggcagct tcttcatgcc ttaactttca gtataaattt taagagaaca atctcgttta
     3541 tgcgagtctt gtacatttgc aaatgcgttc aagattattt catacctcga gatagagatc
     3601 gaatttttct ttgggacaac aagtcaatcg attccaacga aaggccaagt agataaataa
     3661 atggttatca catgtaaata ctagtatagt tcacataatt ctacagaacc catagtggtt
     3721 gtgtttttaa aatctcgact acctgcttac ccgattctat tgtaattgaa cttggcttat
     3781 tatgtgaagt gtatgttgac tttgttctct tcatctgcca aaaagggatt taaaaagtct
     3841 ggttagcgct tgacatgcat agatgtcact tgttgcgttt cattcaccac aaggaaatat
     3901 gtaaggcagc cgtcatttca gtttgaattt attatcctgc ttatctcgtt aaataaatcc
     3961 tcattcctga tgggttttct aagaaacaga tctttatcta gtccacaaaa ttcctctttt
     4021 ctaaattctt cataatacat ttcgctggcc gtcagaaaac acaccttgat tttattgtct
     4081 atctttttca tttcctggta caaaaggaag ccattcattt ccggcatttt gatatctaat
     4141 agaagtaact cgtataagcc agtcttaaaa ttctctaatg ccaaaatcgg atcatcatat
     4201 gaatcagcgc tgaaaccgta ctgctctaaa acctgcttta ggactgtaca aacgtctggc
     4261 tcgtcgtcta ctatcaagat gtttttcatc ttgccattat cctttgtctt tgttgtcgtt
     4321 tttgtcgttt ttgtctttaa tatcacccga cgttgttttt cgctatcgcg tttgttgcta
     4381 gttattggta aagtaaagta aaacgtggca ccgttttgac cgtcagtatt attctcagcc
     4441 catattctac caccctgcgc ttctattatg cttcttgaga ggaacaaccc caaccctgtt
     4501 ccttgaaatg aatttgaagc aaatttctca aataatctgg gaaatatctc gggatctatt
     4561 ccgataccgg tatccttgac actgacaaga gcaacctcct gtccataatc atctttcttt
     4621 ttcatactga tatgtatagt cccaccattc ctcttggtga atttaacagc attacttagt
     4681 aaattggaaa caacctgaat aagtctaaat ctgtctgctt ctaccaaaat agtatcttct
     4741 ataggttcat acgatatttg tccgccccta ctatctaact gatttttaat aatgtcttgt
     4801 acggctccag atattgcctc atttagattt aatagttcct tgtgcatcac tagtgaccga
     4861 ctctcaattt ttgaaacatc taaaatgtcc tctgccagtc gcttcaatct cagtgcattt
     4921 ctcataatga gatctaatga cgcatctagt tcttgttttt tgatttctgt ctttctcgaa
     4981 cgaagaagtc ccaataaccc aagtataggc tggataggtg tacgtaattc atgtgctgca
     5041 atattgataa actgcttttg cattttatct gctcttttca gttcttcatt taatgtatgc
     5101 tcattccaaa gcaattcaaa taccgatctg aaaaaatcaa cgctaagttt gctatttgaa
     5161 taaacagcaa atccaattgc ttctgaaaat gtctcggcct ggggttcttt aacctcagct
     5221 cttaaaaact tttcactatc tataatgtac attccaaatg gcgaatgatt tccatttaga
     5281 actttgacag ctgacgcctg ttcatctatt tttcttataa tatttgaatt ctcttcagag
     5341 aaaggacaaa tgatctttac agttgcgccc ctttgtgacg cgtttatgat atgatcaata
     5401 actcccaatc tgtccactct tatcattgcc ttagcgctcg gaagaagaat tagtgcctcc
     5461 tgttttacag attttgctag attgagaaga ccgctactct ttcacgatcg cggtccgtaa
     5521 agacttcaaa gtattctggc tctatccctt cttcaacttc ttttattttt tgttcagccg
     5581 gaatagcttt gttccagaga gtgtcaaata tatattgttg ttgttccaca atcgttttca
     5641 tgttgctgta gagaagctgt cgcacaggtt ttgcttcctc gagggcagct gcaggagata
     5701 gatattctgt ttcacttact gcaaagttac ctttaacacc gtctaaatga cgcaactcat
     5761 cgatcatttt cattaggtct ttgcagtaat agatattctc tttgttaatt tcagtaacat
     5821 attttacttt aacacctctg tttttaaggt caattgttgc tgtctcgtat aattcaactc
     5881 ccacgcttac tgatgagccc gtagaatctg aacacacgct tattctactc tttgtgttag
     5941 acagaaattg gaatatagtg ttatttgcgt tttcaacgcc atccactacc tgtgttcgtt
     6001 cgtaagggga tagagaggcc atttaccggt acatattcta ttatgtattc actatttcta
     6061 tattatcacg cagaaagtaa tgattccatg tactttatcc atgtcttgat ttggctagtt
     6121 tattcgaaca ttatttgaag gtgaatatct tactagaagg gcttaggaaa tatcaagcac
     6181 ataagttact aggccgtcaa tattttcttc ctcctcatta tgctaaaccc attttaaaag
     6241 ctgctttgac cgctactacg aatttattcg agtcggagac ccatccaaac acccctccct
     6301 gcattctaat ttgctttatt gcagcctgat agttaccata atcagtggca cctgtaccgt
     6361 cttttagtag tgtgcaccaa taaccataat catttgcttc cctcattaca gtatgtacac
     6421 atacatctgc ggtgattcca gtaattataa ggtgagttat gcccaaattc ttcaaaatga
     6481 atggtagatt gctctgacca aatgctccct ttcctgcttt atctatgacg tattctcctg
     6541 gaattggatg tagatcatca attatgtccc agttcttttc aactctcact aacaatcttc
     6601 ctagtccacc tttcggaata ttaccaatcc caactccgtt tccgatgatt ttgcttcgaa
     6661 ggactttgtt gtacggtgcg tctgaaagat ctggtagatg tccttctcta gtat
//
LOCUS       DAOR01000209            5334 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_40817, whole genome shotgun sequence.
ACCESSION   DAOR01000209 DAOR01000000
VERSION     DAOR01000209.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_40817"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1772 a          912 c         1088 g         1562 t
ORIGIN      
        1 tattattaat attattatta atattagcag ggggatgtat tgtagattct gatatactgt
       61 tacctctgtt accttgttga ttcatattat aagtattgaa gttgtttctc actgttattc
      121 ctgtaacgtt ggtaatataa tacgcagtca aattggaagg agtatcttta caagatattt
      181 tagctgttag gttgtttatt ccttttgtaa cgggatgata gtttttagca taggtaaaaa
      241 tccaacttga ataattatta tttccggagg atcctaccgc tataactttt tgaaacttca
      301 aatcatttga atttgcataa acagtgcaat ctttagttgt attatatgtc gatgttccga
      361 atattgttag actgtcacca atcggaattt gttggcgtgg tggagggtat gtaattctta
      421 cattcaattt ggatgatatt tgttgttgtc cctctaccgc gatattcgaa taagagatgg
      481 tatgagccaa aaatacaatt agcacaagaa agtaagccaa aaatacaatt cttttatcgt
      541 tggtaaattg tatcaagagc agctgccggt aattgaacat aacaatagaa taataagttg
      601 ctatagaaag aaaaattgat tatttgcatt tgatgtttag ataagcaacg atggaggaaa
      661 ttttgaaacg gtacctaatg ttttgacata tcctccttct ttaaaggcgt gggaaattct
      721 tttccatcag tagttctttg atccagtagt ggaatgcgaa ttccatcatc attgactatc
      781 aaatccaatc tttgtccttt tcttagattg aaaccgtcta ttacaggctt aggtattact
      841 ataaatgagc tagtacctat ggaagtaatg gtaacagcat atttaacagg cattcaactt
      901 gaaacattag tgaactatca tatttaaata taactgtaaa tataaaccta tatattaata
      961 tctacttata tctgacgtat ttcatggcat gatggcaagt ccaattcgaa tattgatggt
     1021 ttctacagaa tatcctccaa tgcaaggagg agtaggccgg tatacattta atttaacaaa
     1081 ggaactgcga aaaataggtt tgcaagtcta tgttgcttgc aatgaaaaag gtggtggaca
     1141 gttttttgga ctatctccaa ctaacatcca caattctgaa gtactcctgc gtatagttaa
     1201 tgagatacaa ccagacatcg tacatgtgca gtatgagcct ggcttatacg gcttagtatt
     1261 agatccaatt aatccaaaaa agaccagtac taatattgag tccttctacg ataaatgtaa
     1321 aattccgata gttactacgt ttcattctgc atatactgtc aagcaatggt ttagtctggg
     1381 gccaacgaag acgaatagaa gaaatggaaa tgcggctaaa ttactgcttt gtagagttgg
     1441 agattactgg aagcgccttc taaactatcg atcttttcac aatttgaata agattaagct
     1501 caaaaatggc caatcaggca tagtcttctc gtactatatg tcaaagatga ttgacggtgg
     1561 agtggataag gctgatataa agataatcta tcatggcgcc gaaccagctg cgcgcataac
     1621 gaaaaaagaa gcgagggcga gattctctct accacaagaa ggacagatag ctttggctgt
     1681 aggattcaga acagctacta aaggctggga tattcttgaa aagatgaaag ttccaaaagg
     1741 gtggactata gtagtaagct cctcagagaa tcattatatt aaatacgatt tacagttacg
     1801 aattggtagt aatagtaata tcattatcct acagaatgat tttctgagcg aatccgatct
     1861 atcaatctta ttctatgcct ccgatgcgat aattttgcca tataaagtga gctcggcttc
     1921 tggagtaatg tttgatggct tggctcatgg actaccgttt gtagcaacag atctggagtt
     1981 ctttagagaa ttctctgctc aagggttagg aataacagtc agaaggaatc ctagtgcgtt
     2041 tgcaactgga cttgtaagct tatcttccaa ctatgcatgt tatagggaat ctgtcgatat
     2101 atttaagaaa aaaataagtt ggaatgcagt agccatacag cacgccaatc tttacaaaga
     2161 gaagagggga tcttaactct atggtaacat gtgaaaaatg tcacaagaac gaagaatatc
     2221 atacatgctc gacatgtaac cagcatttgt gcattaattg ttttaacatg gttcattttg
     2281 gcataaacaa tccgagaact ggaaaatcga tggtatataa caaatgtata ttttgtagtg
     2341 atatatatgc gacgaatgca aaaagtagcg tctgcgataa atgtgtgatt gtacttacgt
     2401 gggtatcagg catgaattac cttgctgcgg taaacagagt ttctgcctta atcaaatcaa
     2461 ataaattgga ttcaaagctg agaaatgaga actactgcaa gacgaacttt ggttcgcgtg
     2521 caacccaggt aataatggat gcaatagaca gctccaaatg aaatggatcg tggtggcaga
     2581 ttcgatataa cttgatgttt tctattcgat gttgatctgc attccttttg gctcaccaag
     2641 accacgacag ctaccaccag atgagaaatc tcccaaatca gtcaaatggc cttcttctta
     2701 gtttgctttc actgtgtaaa cagaatatta ctatttgcca attttttgat ttttagttag
     2761 aagttgtacc tgccatctga ggatagcttt ctccttgcac aggctgttaa aggttacacg
     2821 gggaaatcag cgttagaaat cggagtcggt tcaggggtcg tactacaagt tcttcatgaa
     2881 aattttcaga ttgttgctgg aacagatatt gattttgtat cacttagata ttgcaaagag
     2941 aatctgtcaa atgatatcat gctagcttgt tgcgatgcag cttccgcttt tcggcataaa
     3001 tttgacctga ttgtaaccaa tcccccttat ctcccactag aagataacga aaggaaggat
     3061 tctgcaatac atggtaattc taacggtata gaaacaacat taaatttcgt aaaatctgct
     3121 atttcagttt tagccgataa tggtaaaata ttgataataa tctctagttt gtccaactat
     3181 acaaaaatag acgaatttct ggttcaaatg aaattaagaa gacgaaccat aagcaaaaag
     3241 aagttatttt ttgaaatcct cacgactctg gagataaggt ttccataagc cccaatctct
     3301 gtacagcttt tgttacctcg ctagatctgt gaagaatagg tctaccacct gttactcagg
     3361 tcgcagacaa cctacttgtt gttattcata gctgacatct ccaaaagctt cgtaatcttt
     3421 gctttcgagt tgttttttcg ccaagatatt caatgatgca gatccatcct attatttact
     3481 cgagtggctg ataatctcgg taatgatgct gtgcatgatt ccttgttgtg tttgataagt
     3541 ttgattagcg agcccgcggc caccagagac tacaagagca cgcattatta gggaagcttt
     3601 ctcaagctag tacctctcgc aatcaggtaa accgttaaga acaacgaaag gcaactccat
     3661 aggaagaagc gatggtcgca ggaaatgaat ccttccagta acacggatga tgtagatacc
     3721 aaggaaattc acattatatg ttcgctgtgt ggcaagaggg cgtcaggaca aggagaatct
     3781 accaggatcc aggaagtaat tggcggaacc tattatacgt ttgatacaca ggattgcctc
     3841 aatatgttta aaaggttcaa gagtgtatat ggcaatgaat ttaaaggatt tcttgcacag
     3901 gaacaattca tttcagaccc tttttgggat agagcaattc caaaggaaga ggaaattaga
     3961 gaaattgaga ttgagaaagg aagagttaaa ccagatatta tacaggttat tcaggacctt
     4021 ggtgagatac gtaaaattgc ttcagaaata gtaagaacag ctcgacatga aatcctgata
     4081 atattttcta catctaatgc atttcaccgg ccagaacaca ttggaggaat tcaattatta
     4141 aaagatacac tcacaaaaac ttcaacactg gcaacaacaa agcgagatct gaatatcaga
     4201 attttaactc ctcccgatga ccaattaaaa gctacatctc ctgagttgaa ggaacaactg
     4261 aatatcgact ttagatacat tgaagaatca ttgcagacca agatgacaat attggtagtt
     4321 gacagagtat tttctttagc ggtagaactc aaagataatg agagagatcc tacctacgag
     4381 gcaacttact caagaagcaa atcaactgta ttatcatatg tggcgatatt cgagagtctt
     4441 tggaaacagg tagaattgaa tgaaaatgtg aacaaacttt acgaacagct aaaaatcgag
     4501 gacaaattgc acagagactt tatcaatgtg gcggctcatg agttaagggc gcctatacaa
     4561 ccaatacttg gattgactga agtaattcgc tccaggcagg ggaagatgaa cgccaatgaa
     4621 caaggggaac tactagctgt tatcattagg aacgcaaaaa gactaaagac gcttaccgag
     4681 aacattttgg acattaacag aatagaaagt cgatcactaa gcctgaataa ggaaacactc
     4741 gatatagatg atgtaatcct cgatgcaata caagatattg aaaatcagat tgataataaa
     4801 caggacctca gaattttgca cgattctaaa agaaaaggag gagaggagca agaaggtcgc
     4861 acttttatga aagcagacag aaataggata acccaagtta tttcgaatct gttgagtaat
     4921 gctattaatt tcacaaagac aggtactatc catgtctata aggaaacgga aaaagatggt
     4981 tctatgagag ttattgtaaa ggacactggt actggtatag accctgaaat tctgcccaga
     5041 ttgtttacaa aatttactac caagtcaaac aaaggtaccg gattagggct gttcatctcc
     5101 aagaatatcg tggaagcaca tggtggaaaa atatggggcg aaaataatcc gaagggaaaa
     5161 ggagctgtct ttgtgtttag cttaccacga gttaccaata gataggtggc agcagctcac
     5221 gtgcgcaagg ctgacaggca acgtcgatca atcaggaaca agcaatatga cgtgatctcc
     5281 aagtgacata gaggtcgttt aatgagactc gtacgcatga tttgtacact tgta
//
LOCUS       DAOR01000210           28995 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_7037, whole genome shotgun sequence.
ACCESSION   DAOR01000210 DAOR01000000
VERSION     DAOR01000210.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 28995)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28995)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28995
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_7037"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         8399 a         6005 c         5951 g         8640 t
ORIGIN      
        1 cttcagtagc tagcaaaaac tatcaagacg tacgaacttc ctaatttttt gttgcaaaat
       61 caataagcgt attattttta actttagatt cgagggtttt tcttagaata tagcacattt
      121 ccctcttttg tgatcttcat aaccctattt ggcggtataa gttcaaaatt ttcagacaca
      181 tttacgaatt ctgagagtga aaccttgacc aatgaattgt aatctcgata atagacggaa
      241 tagagttttg gatcgtcggc atagagtgct ttgctgaaaa tctcttctag cctacccttc
      301 ttcggcattc ctgtagtaga tcaaaccccc tgcctccact atctgcgcta ccaatttcgg
      361 aaacggcacc atcacataag attcagccct tgttagattc acgattttgt tttgattgag
      421 atcgacttca agttcgtcct tatcgaaaat tcttcccaaa gtttgttgtt cgatttctat
      481 gggaagcaaa tagcctccat ctatggcatt tctgtaaaat attcttgcaa atgatggggc
      541 taaaatcgct ttaataccca actgagaaag agctatagga gcgtgctctc tgctcgatcc
      601 gcagccgaag ttacttccgc ataccataaa atcaccctcg gaaaccttct gtggaaattc
      661 cggatctaaa ccttccattg catgttttgc caattctttg gggtcatgaa tttttagata
      721 aggccctgga atgataacat cagtatcgat gtttgacatg tcatatttat gaactctccc
      781 ttttattgaa ttcaatctac gatatgtccc tcggatctgt tatctttcct ctcacagcag
      841 aagctgctgc gaccataggc gatgctagat acgtctttga cctgacatgc cccattcttc
      901 ctggaaagtt tctatttgta gtactaacgc atatctcatc ttcggccaag actcccatat
      961 gagcaccaca acaagctccg catgtcggcg gtccaataac gaccccggct tcgagaaata
     1021 cactagctaa cccctctctt actgccttga tgtagataga ctgagcggca ggtaacactt
     1081 ccgttcgaac ctttaccttc ctgcccttga gtattctagc caccgcttga aggtcctcaa
     1141 atttagcgcc tgtgcacgag ccaatgtaca ctttatctat ttcaatttct tgtaattctc
     1201 tagctgcgct gatatttcca ggagagaacg gtttggcgac cgtgggttct aatttgtcta
     1261 tctcataggt taatacttct gaaaatttag catcgctgtc gcccaagatt ggcgaatatg
     1321 aggctttagt tctttcccgt aggtatgact ccgtcacacc atccggctca attattccat
     1381 tctttgctcc ggcttcagtt gtcatattgg tgagggtgag acgctcgtcc atactcattt
     1441 ctttaactat gttacctgaa aattgcattg ccttgtagtt tgcggcatca gttcctatat
     1501 caccgataat tctcaaaatg atatcttttg ccatcaagta atctggcttg gtcccggtaa
     1561 tctcaaagag cattgtctcc ggtactttca accaaatttt gccgttcatt agaacataag
     1621 caatatcagt atgacccagg cctgcagcaa atgcccctaa cgcccctgta gtattggtat
     1681 gcgaatcacc agcaacatat acctcgcctg gcaacaccat tgcctcttcg tgagacaacg
     1741 tatgacagat tccatattgg ccaagaccat acttaaaatg ttttttaatg ccatatttct
     1801 ttgtccatct cgatagaatc attatattct cagctgacac tttgtccgcc gcagggacga
     1861 aatgatcttc cgctatccac actctgtccg gatcccaaat atgatccagt tttttaccct
     1921 ttttttccca ctcacttaac accttaataa cgcctggacc agatacatca tgcatcatta
     1981 ccttatctac acttacaaac cctacatctc cggcttgtat tctcgttttg ccgctagcgc
     2041 gggcaagtat tttttcagta attgtcatgg acataggggt tcaccttgca gaatatccgg
     2101 cacgactgac ggattaaaac cttctgcaaa gaaaaagaaa acaaaaaaag ggaagtgacc
     2161 gtagcttctg gttcacttgg aaatattacc aatcctggtg ccagtaaaga caggcaaatt
     2221 tgatctgtat caaagcttca gggtgtccgg ttttgaattc gaagaaaatg atattctcgt
     2281 agtttccagc aaatacatat ctatgagtga gggttcggtg gtgaacctca ataaagtaaa
     2341 gtccagcaaa aaagctaaaa tcattagtgc tgcttgccac ataaacgaaa aaatcgttga
     2401 gcttacactc agagaagccg attatatatt caagggagtg cccggttttt tactatctgt
     2461 taaggatggc atattggctc ctaatgctgg cattgacaaa tccaatgtac cgaaggggtt
     2521 cgttgtctta tatccgcgtc ttcccttcaa aaccgccgat aacttgagaa ggcgattcct
     2581 cattgacatg agtattaatg ttatgattgt ggtagcagac agtcgattga tgcctggacg
     2641 aattgggact atcggtgtgg caattgccaa tgcaggattc gaacctactg aagaccaaag
     2701 gggaaataag gacctattcg gacgagtcct tagagttagc tttaaggccg tctccgatag
     2761 tctagccaca atggcggtcg cagttatggg tgaaggtaat gaatcgatac ctgcagctgt
     2821 tattcgagga atagaagtaa cttctacaga caggaaactc tcatggcttg atacgacggt
     2881 ttgggctgat caagatctct acctaagggg attacgaagt tgaccgactt ttcttacatg
     2941 atcaatttat atgatctcgt agcattttaa ttgtctggga tattgaacat gtgggtttgc
     3001 taattcatgt ctgaattcct gactagatac cctaggcgga taagcgtagt aaaaggactg
     3061 catgatttca tacgtcacga ggaaattcag ttcgatcacc ttagcttaat ggtaaggata
     3121 aagaaagacg aaataataaa gactctaatt tctgagggtt tcaggcgcgt taaacttgag
     3181 aacaaaaagc cctctcagat tggcaatgga ttcgccaaga aattaagcaa accttgggaa
     3241 atgcatgtta ggcttttgga agtacatcag caaggattaa ttgcgattca ggctgaagta
     3301 gaaatctcta gaagatatat acagcatatc cgttcagtca gggcgccggt tatctatgaa
     3361 atcgagtcta ttcttcggaa gcatagtata gaatatcgaa tttggaacgc caaggtcaga
     3421 gattacattg ctagtattat cgataatcat cagataaagc tctctgcacc aaaattgcca
     3481 gctatgccat ggatacccat gctatgcttt gctataacca tagcgctctt agacgcattg
     3541 aaattcaccg gagtcttgcg gtagcatata ttccgcaaat gttcgcctga ttttaattct
     3601 gcgacttcga accagcagta tcgtctgagg agagtagggt gtgcaagata actagctaaa
     3661 tagctgattc accacgagtc ttcgatccaa tatctactgc atcgtctacg tccgatacga
     3721 agatttttcc ctcgcctgcc aggccggtac tcgttacctc aagaatttta ttcacaactc
     3781 tgtcaacaac agagtctttt accacaatga acatagtcga gtttgcatta aattctgggg
     3841 taaaagttga agttcctctc gcagatgcaa gctgaggtct agggagctgt cccctgcctt
     3901 ttgattcgaa gtaagttaaa cccccaatct cttccgtctc cagagccgcg aacgctttgt
     3961 caagtctccc agatgggaag accgcttcga tcttcttcat aatctaatac ctaaagtcga
     4021 atgaacaaca gttttattaa aatattgtga atagtaatct tatgtgtgtt accaaaagtc
     4081 tccgataatg actgcaaact tacaggtaac gtaggcaaga gagggagaga aaccgatcaa
     4141 atatctcggg ctaatttttg ttaagtaggt ttttagtatc gaaaataaca atatctctac
     4201 caggtaaaca aatttgcatc tgtttaggaa gaaagagatc ctccagtgca gtaattgtga
     4261 taaaatgtta agtcatagat acaagcctcg acccgaatgg aaggtccaag ggttcttttg
     4321 tagcggatgt catatggaaa agactaagga atttattcta agacaacaag aagagaagac
     4381 aaagcttgaa caggcccctg acatttgcaa tctctgtaaa aaagagctct tgcttgaaac
     4441 ggataagaat aaaccaagct ggcaatggaa tatggagcgc gagagtatac tgtgcaaaag
     4501 ttgttatgaa aagcaagccc tagactacga gaaaaagatg aactattgta caaggtgtag
     4561 caatagattg gcgtttatcc gttacaatcc aaaaccacga tggaatattg aaggccagtt
     4621 atgtagaaaa tgttgggaca agcagaatca ggtaaggccc taaacaatga tggacatctt
     4681 caaaaagcac acctgtgata gatgtggtac gaaatttcgg agagtcgagg cgctgatgca
     4741 acaccttcaa gtaatacatg gaaaagggtt attttatgag tgcaaaccct gcaacgcgaa
     4801 attcgatggt atggaacaga tgcgcgacca tctaaaaaaa tttcactcat ataacaaacg
     4861 agggataagt gaaaaaaaga gaacataaaa gtgacataaa gaataaaata acctggtgaa
     4921 ggcatgttct tcacatggta aaggtgtgtc tcaaatgcag taaagggttt gatgaaaaac
     4981 tgattgatga cccaggctct gaagcatatc cttcttgtcc ggactgttgg aaggaatgga
     5041 ctaattatgc tgttatggta atgaatgaaa tgagattgga tatgtctcta cctgagcata
     5101 ggaaagcact tcgaaaatac gaaaaaacgt tctttgggtt agaaaaatca gaaggcgaac
     5161 tacaaaaaaa tcctgataaa cgtccccagc aataagacgg gaactatttc atttccaaaa
     5221 tccctaatgc ggcagcgggc atcatctcta tgattttatg cctatttggt acagacaaca
     5281 tagcctcctt taggcaatca atgaatttta gttttttgac ttcatctaaa gtcataaatg
     5341 cgtgaatcaa acagtcaata tttagtttct ctattacaga gtggtcgttt agaattcctt
     5401 cacagtagag tcttaacatg agaactattc tgctagtgtc aaattcggat agaacttcca
     5461 tccaagtctt tgctaacaca gcgagcttct cagcctggat cgaagaagcg gcatttagtg
     5521 ccatcgctat cgtttcgtgc ttatcggatt cgtcaaatat ataaaattcc ttcagtttct
     5581 tgcgaagaat tggttctcgc agaaaatctg gcagattagc cagaatttgt atgatattca
     5641 cagctgcttt agaaccggat gtcaccgaca gtttaagact gatattgatg cagtatttaa
     5701 ttctatgaaa tagtaccaga ccgcgtcatg tttcggttgg taccgttaat tattcattcg
     5761 cgcctaaact aggcttaaat attcacggat ctcaagagtc cccatatgcc atgtcttgta
     5821 atcgtgggag ggttcttcgg agacgagggc aaaggcaaaa ttatcgccta ccttgctaac
     5881 aaagaaaacc ctgtcattgc cgtaagaggt ggagtaggtc ccaatgcagg tcataccttc
     5941 tgccatgaag gcaagaccta taaagttagg atgctgccaa gtgccgcctt gaattctacg
     6001 acacaattat taataggagc tggtgtttta gttgatccaa atatattgtt agatgaaatc
     6061 aagaccttcg gtgtgtccaa tagaactttg attgattcgc agtctggcat aatagagaaa
     6121 gtgcatatcg aaagagacat tggtggcatt catctgaaaa ataagatagg tacaactgga
     6181 acaggcactg gtcctgcaaa cgcagacagg gcaattcggg ttctcaaact tgctaaagag
     6241 attcccgaat taacgcgcta tattggagac gttagtggta gtgtcaatga tgccttggat
     6301 aggaacgaaa gcgttctggt tgagggaacc caggggacat ttctgtcgct ataccatgga
     6361 ggttaccctt atgtaacctc caaggacgtc acagcttctg cgatttgttc tgatgtcgga
     6421 attgggccaa aaaaagtgga tgatgtcctt atcgttttca aagcctatat tacgcgagta
     6481 ggagagggtc cattgaaaga tgagattacc agtgaagacg ctaaagtgag gggatggtta
     6541 gagtacggga gtgtgaccgg taggcaaagg agagcagcgc cgttcgaccc agttattgcc
     6601 aaaaaggcaa taaggttaaa tggcgctacg caaattgcga tcactaaatt ggacatcgta
     6661 tttccaggtt ccgctggtgt aagggaattc tcaaaactac cgagggaagc gcaaaccttt
     6721 gtcgaaaatg tagaaggaga aacgggacta agagttacgc ttataggaac gggggcagaa
     6781 cttactcaac ttattgaccg aagagagacc agccagattg cattatgata gcttagcaat
     6841 ggagcttctc aagggagggt ctagtggcga ctcatttatc aaaggaattt ccactgtata
     6901 attcgcgtct gtcaattctg cgaaagattg ttagcgtttt ctcaagaata tcatcatctc
     6961 cttgactaac gtgataagta gcccacgcta agattttacc cgcagtttgt tgggaacaat
     7021 atttctttag ggatatgttg aattcttcag ttgtagattt tctttgtccg aactcatgaa
     7081 aaggatcact ggaggggcgg ttattatcag ctgcccaagg tgttggtcgc gaatgcagat
     7141 tagggtcatc atatgatttt tcacatgtat tattgccacg ataggtacta taatagatac
     7201 tattcatcga tggttgcgat gtgttactgt cataacctat tatatcactc gttctgtctg
     7261 tgatcacctt agaccaggac ttatcattat tataattgga gccaaataga ttgatttttc
     7321 tcattctccc ttctggagga attggccact caaccttccc acgatataat cgaggatacg
     7381 aacgataatg ccttttccat catgtaaatc cctgttgtgc ctattttcgg tatatctgct
     7441 agtaaagtgc tgcgcacata cccaagtgcc ttttttgttt tctacctatt ttaaattgtg
     7501 atttctctta aaagaaaata ataaaaccag caacagcgtt gcactgtgct gcactcttcc
     7561 ttcaacgaag gtaaagtaaa agcatttgct agagaaataa agctaatcaa aaataaagaa
     7621 catgaatatg tatctatgta atagatcatt atactactcg aagttctaag tacaagtgta
     7681 caagactatt cgttttcatt cttaatgcgg tgcgagccta atccttgcat ctcatttggt
     7741 tggagataat acgcgttaac ttagcaggtc caacctatac aggtacatta tcttggtttc
     7801 gaatgacttt taatagcatg tacagtatga atagtaaaat tgcaacttaa gtctccgtgg
     7861 tatatagagc tgttgaattt gaataatttt ggcagattgg tctgcgcatt agagcgcacc
     7921 ccgatgcccg cattctcgtt ggttttagat caacaatttg tcttcgcggt tcaaacggat
     7981 tttatagagg ggagaccggt aatctatttt gtaaagagcg aagaaacgaa atacggtcag
     8041 tatatggcat acaggatcac cggggtgaca gaagaagttg ctgtggtcga ttcagtcgtg
     8101 aatccgagct tcatttacac accgataatc aatgttgata aattccccgc aactctaaca
     8161 agaaaagcgc gggtaaataa aaaatcaggt tatatcacaa taagactccg agacctctcg
     8221 agtcttgcaa aagttgcagc ctacaagaca atatacgacg aaccaccatt accgctattg
     8281 atgtttaaag agaataacaa gtttgtgatt gggtccgcaa tcagcttaat cgaatctgat
     8341 gcgatatctt atttctatta cctcgttctt gatgaagaac caaccgatcc ttttttgcga
     8401 tattccagtc aaaaggtaga acaaccatcc ttttctaata agataggtga gcatggctac
     8461 atttatctca agttaatcaa acttgcatcg agccatcctt tggtgaaaat ttatggttga
     8521 ctcagcagcg tttagtcaca ggatcactga agcatccata atcaaaagaa gcaggataat
     8581 tctggcactt gacttaacag ataggtctga tttgaacgag ttcgctttaa ggacgatatc
     8641 tcagctggaa agctacattt gtgcaattaa actgaatttt catctgatat tgccactatc
     8701 aagctctgag ctatccgaag taaatcgact tgtgcattct tatgacctgc aatctattgc
     8761 agacattaaa ttaaatgaca tcccaagtac taataaaatc gcgatatctc atctttcgag
     8821 tatgggattt gatgcaataa ctgtcaatcc ctttattgga accacaggac taagagctgt
     8881 agtagtgcag gcacatgaat tgaaaagtgg aatacttgct ttggtttata tgagtcatat
     8941 cggagcacag ggaggattcg gtatcaattt cttaggtagt aataatcagg ttacatcgtt
     9001 atatcaaaag ttctttcaag atgccattgc ctgtcgcgtg gatggagtta ttgtaggtgc
     9061 aaatcgactt gaaattctaa aagaaatctc ttgcaaaagg gatcgcattc ctatctattc
     9121 accaggagta ggagtacagg gcggtaacat aaagcaggcg gctaacagcg gtactgatta
     9181 ttttatcatt gggcgctcaa ttatcggatc gaaagatccg ttgaagactg cgaaaacctt
     9241 acaacaccaa ttagttcgtg ttaatattac agattaatag tgcatgttat agttgactgt
     9301 cagtctcatc aaggcgcgga gggagacctt tttatgttcc aaaaataata ataacaataa
     9361 tagaacggat cgatcaaaga tatctcctgt caaaacattg aaccaggccc gtataacacg
     9421 ataatcaacg aatctgggtg attaaattaa ttaacaagga gagtcattgg aaggttatat
     9481 agtactaaag ggtgttcaga atgcctagtg ttgataacta cgcgaagaaa aatggagcag
     9541 gattatctag ggtagcagta acagccgcat caacacatag cagaattctc gttttatgtg
     9601 tagatcgaga cgatgatatc ggctctaaag gcggtatgga aacacctgtt gtgggaagag
     9661 acccatgtat caatgctggg gtaagacttg cgatagaaga tccagaagat ccagacgcta
     9721 atgccatctt tgcagcaatc aaaacatacg aggaactcat tgctaaagga tacagctcac
     9781 aggttgccct tgtagcaggt aagtttaatc gcgggattga agcagacgag aaaataggtc
     9841 tggagatccg gaatgtcctt ggcgagtatc gggcagacgc cgctgtaatc gtctccgatg
     9901 gcgaagatga tgagactgtt gtgcctgtga ttcaaaccat ggttcccgtc atttctattc
     9961 agcggataat aatcaaacat agcagaaccg tcgaatattc atacgctgtt ttaggaaggt
    10021 acgtaaaaat gcttgcttat gaccctcgtt attcgaaatt ctttttggga gttccaggcg
    10081 cactcctagt tgccacaagt cttgcgagct tttttgggtt aacgaaagag gctctagcct
    10141 tagtgttcag tattttagga atagctttca tcattagagc gtttgatata gataaagcac
    10201 taagttctct tggcagaccc acaccaactg gttttattcg aattttctct gcttttgccg
    10261 gaatattgat cattgtggtt tccatagtaa atggcctttc gagcattcct aatgaagcaa
    10321 ttgtgccgaa tatgggcgct ctgcatgtta taagtaacag aatcatagtt ggaagttttg
    10381 ttcatggtac tatcactctg ctttggatcg gtatagcgac tatactagcc ggatcgctat
    10441 tgagtaactg gttcaaaggc agtatcctga caatgtccga tattttacga ctcgtagtgc
    10501 tggcgctatt gtatattcct atcctgcagt ttacatctgt tctgaccgaa cgaactaatc
    10561 cgtttagctt aatttcctcg ttgttaatag gattggctat aactttggtc gcggctacgt
    10621 tcttgttcca atttttcagg aacaggaagg gtggagaaga tctcaagcat taattctcga
    10681 aatattgtaa ctaattgttt caaaacagta aaatcattgt cgaacacttt gtgtattaat
    10741 cgtgattcaa gatattggca gtagggctat tgcggacact ttctttaagc gcagcggact
    10801 ctacaatctt tatgagcgac gattagagtc tcaaattcta aagaactcgc ttcccaatca
    10861 tattgcgatt atactagacg gaaacaggcg atgggcaaaa tacaacttca tgcgagcagg
    10921 aagcggtcat tttatgggcg cagataaagc tgaggaacta ttgaattgga ttcatgaatt
    10981 aggtattaaa attactactt tgtacgtgtt atccacagag aatctggata gaaaagatga
    11041 agtagataat atttatcgac tattacagtt aaaactagaa aagctttaca aagactcgag
    11101 aatacaccgg cgtcgcatga aaatcaaggc aataggaaat gttaaactgc ttcccacgca
    11161 acttcaggaa atattgaata agctagagga agacacggca gagtataact caatgtttct
    11221 aaacatagct gttgcctacg gtggacagga agagctcgtg gatgctgtaa agaaaattgc
    11281 agtaatgact aaaactgggg aactctctgt ggaagatatc gacgaaaagg tgatcgaatc
    11341 ctgtctctac acatcccatt tgccacagcc agaacccgat ttgatattga gaacctctgg
    11401 agaaaaacga ctaagcgggt ttctaatatg gcaaagtgcg tatagcgaac tagtgtttat
    11461 ggatgttttc tggccggaat tccgcaaaat agacctgatg cgggcaatca gaacatacca
    11521 acgaagaata cgaagatatg gcaagtagcg tcgtgataag aataagtatg agtcgatttg
    11581 atcgtgatga atatctagaa atgcctgagc atggagatta tcacgctgga tcacgcagat
    11641 ggtattgtaa ctactggcaa tcttcgaagg aatgggaaga tattcatgat tatgctccac
    11701 cggatccatc aacagaaacc acggatgatc catttcgtag tcatacagcg gaaacgcttg
    11761 aaaaatgagc aaatgggctt agggcaaaaa agtcagtgaa atcctgtggg tggtatgtgg
    11821 tatgtttgat gaaaactctg ctggtgctat attatattca ctcaatgagg atttgctgag
    11881 aacagaatac ttgttactgc atcatacttc aggtcattgg gactttccta agggaaacat
    11941 cgaattaggt gaagacggat tccagactgc acgtcgagaa atatatgaag aaaccggaat
    12001 caatgacatt gaatttgtta aaggctttat atcgaccata gagtactatt atagacgaat
    12061 agataaacta gttcataaac gggttattta ctacattgct aaaaccaata cgcgcgaagt
    12121 tatactttca tccgaacacg atggatacgc ttggaaggaa tacgacacag caatcaatca
    12181 attgacgtac aaaaatgcaa aacaattatt aaaaaaagcc aaaacgtttg tggctccaag
    12241 atgctgagtc agacggggta accatgagag gaagtaattt acggtataat atatacaagg
    12301 aaactggcca agataatgcc tgcgacaatc gcggctgtca agaagatccc ttttttacta
    12361 actcttctct gtttgccgct tttaccagta ataccgtcat tatgatcgtg tgccaatcac
    12421 cgaagtaata ctgtccattt tatataaatg gtagcacatc ttcaaaaagt aatccgtctt
    12481 cgaagttgta gttatcccgc tatggccttt gtcaaatcaa gagtgacttt attttttccg
    12541 ccctgtttca tattttgaac atctgtacaa atctgagtgg caggcggtgt caccgcggtt
    12601 gcacatatcc gtatagtgcc atttatgagc ttgtctggaa ctgtgatagg tatgaccaaa
    12661 ctattttttt tagttacatc gatattcgac gccacgacgg tatcgttaat ggatatattt
    12721 accggtccat tttgtgtatt actacgaacc agaacaacgc taaactgtgt cccgttgcta
    12781 tgagacgtag atgctgctgc aagtttcata gaaagagcag cagagctgta cgagattact
    12841 aacatcatta atattgacaa aattaaaact tcttgtccaa ctaccttcat attacgaaat
    12901 ggtcattatt ccacatttaa gagcattgaa ctaatttatt agacgtcgca agaaggttta
    12961 tttcacaggc tacgttacgc ggccagccag cctgattacg tggcttagtg tatagttaat
    13021 aaatgctaag ctgtttgtca ccaagattgt tagtagccct gttaagaatt aacatcttgg
    13081 cgcatataca gtaaacaccc aacagattaa taatgagcag gaaaaaaata aagattcacc
    13141 ttgaggatgc ggatggagga aaatatgatc tagcattaga aggcaatatc tcaaagacca
    13201 agattatcaa attcattgaa gttctagatc tcctagaagt gaagggcaaa gacaattttg
    13261 aggataatgc aaaactcaga gagaaagata acttgacgtc tgtaggttcc aagatatgga
    13321 atcttgtaga acgaaaattc ccttatagca cctttacttc atcagatatc ctagaaatct
    13381 acgaggatga gtatcaagag cccattcagc ttagcgtggt tgcaacatat ctatccaggt
    13441 attctgaaag gagcaagctg tccagaaata agagagggaa ggaatggttg tacaaagtac
    13501 ttagaaaaca tccagtgctc gagcaaaggg aaccaactta cgaacaccct cacagtatat
    13561 ctcatgaatt ccatccaact gatgtaaatt aaaatcctga tcctgactga ataaggtaga
    13621 gccaaaagga gagtgagaac tgtctcagtt ttttcgtcta ttctaagtgc ttttaattat
    13681 aagttagatt ttgtgttcgt ttcggcgatg ttgatctaat tccatttggc tcttgaactt
    13741 cttcccacag ttatcgcatt tgaatttctt gccgaacaaa ttgaattgca tattattcaa
    13801 taataatgac gagatcctta catatatcca ttatgtatga aagcgtttga tatacggaat
    13861 cgtctcttga gaagaccccc ttttaatcgc caccaattct ggtagaattc tcagaagctc
    13921 ttctgcattt ttggtgaacc ctcattatcc agataatcgc acagagattt tatcatcgat
    13981 cccattttat acattgtagg catcacatcc acatggacat cgttttcttc tagggctttc
    14041 cgagtagaag gcccgatagc gactacgatt acacctctgt ttagagacgt tagtaacgat
    14101 tcgttcagac cgtgttggga cgctatcttg aaaagttctc gcgcagctgg ggggctcgta
    14161 aatgttattg cacctatatg agcaatagaa atatcttcaa tgagcttgag cactttagtc
    14221 tcttcaggag ggatatattc ataccccatc gttttgagga tctgcgcccc attttcctta
    14281 aggtcggttg agtacatgta tgtcgatgat tcgaatacct gtgccccttc cacttcaagc
    14341 tcggtcttta attctgatga atatgaacca tgccagagga taaaaactct tttcccagct
    14401 atgccaagac ctcttagcag ttgagctatt cctttggaag tattgtcctt tggtacgata
    14461 ttagttttta tcccgtgcat ggctaatgcg tccttaggct tcaaactcct ggcgatgact
    14521 gtaacttgct gcaatatttg aatcagctcc ttttctattc ccagattcct tgcagcagag
    14581 attagagagt aaacgccagg tccagtcatg aagactgcat agtccacttt ttcttttaat
    14641 acttgatatg caaaggatcc agcttcctca tttacccctt gatttacctc tatcccaatg
    14701 gtgggagcaa catagggtat acctccaaaa gagattatga tacgagcaag ctccgatgct
    14761 cgacgactac ctgttacgac gaccactaaa ccagataatg acatttccgg gcccgaagaa
    14821 tgatcctgaa gctgaaatta aggttttttg taggacgcaa aggaatcact tgaggaggaa
    14881 taaaatgtac gaccggattg gacatatgat ttctaatccc atagaaaatt aattctgact
    14941 acccaactcc tctccattat ttcattttct tgacaatgaa atggaataaa tttccttctt
    15001 ttcttgtttc caatagtgat tgcccagttt gttcagacca tcgtaccatt tccaaatccg
    15061 cagtggtatc atcggacact agatgtacga cgttaccaat tgctattttt tccaaaagtt
    15121 cgtcaagttc tgtgagtgtg gctgcacatt cgactcctat ctgaaacatt tcatagtctg
    15181 gaaatgcatc aaagcaaata atatggtgtt tttgaatgag agtagccatc ttctcgtaac
    15241 agctttctat agtctgcaag tttttaattt cctcctcaat gtttgaaatt tccattttcg
    15301 caaaccatcc ttcagcatag ggtgaatcgt ttgctaactc cggttgatca attatcgcgt
    15361 gattaacttc gataatccta ccacttatcg gagctcttac tacaccgaaa tatctagggc
    15421 tttcaagcgt gccaaagctt ttccctcttt ctatatatga accgacctgt cttaacttga
    15481 taacggagag tttgccactg atatatccta ggatgcttgt aatcccaatg tttgcttttc
    15541 gtttgtcatg tcggatgtca gcccaaacaa aattctcagt gtcatacaaa atatctccag
    15601 ggaagataca atgatctatt ttcatcatag tcgtagatat tgggcaccaa ctttattctt
    15661 ttatattgaa tttacgaaaa aacatagggt ttagtctcga tctagtagtt tcatttacga
    15721 ggatcgctca atttaaattg gttcaaaaag tacggttaaa gtatcctctt cactgattca
    15781 gatttcaaag acctgtctat tagcttgatg ccttttgacc tgactcattc tagggatttt
    15841 agttgctcac agtaatgaga atctaacgat gcaattttca aatagtatga gaagcaatct
    15901 aatctaatgg tacaaaggca agtggctttt gaaaacacca aacaacaaca ggaagcgcta
    15961 actttgagcg atatctcacc taaatgggcc aaacgactag gagaacgtca agaactacct
    16021 gttcctatgt caattacatg gctatggtgg tattttgaac ttaagcgtgc gtcaaggtgt
    16081 gtagtaggag aagcacatag atattcagca tcttacatgt atgactgcag agaatgtgac
    16141 aaaataggct ggagatttat gcttcatttt accgtgcatt catgttctaa actagaaggg
    16201 aactaggaga ggtttgtcaa gcattggaac agaaagcacg ctcgttcgac taggggtcaa
    16261 gattgtctat ggtagaagta gtcgtatagg ttatgaccgc ttgtgtttac aatagtaaat
    16321 gagaataaga ttattcagat gatatcctca gaatggagat agagtcggtg gctgttgtta
    16381 ttattagctt gtgaagattt ttggtgtacg ttgttgaagc agatcgctat ttctctaacc
    16441 cggtagggac agcttttact ggctatatga tcaaaataaa gctgacttgg ttggtatcta
    16501 ctatagtttt tcggactgtc tgttaatgca gtattcaaag gagtgatacg ccaatagatc
    16561 ccatctctaa tatagaattc tttaccgatt actcatatgc tgtcaccagc cgtgcgttta
    16621 gaccgggctg gtttaactac aattaactga aggtaaatat ggcacgcaaa cttagacttc
    16681 gtttaggcac atcaatccaa aaaacaggca cgtcccttga catcctctat tatagcaaag
    16741 aaaattgcta agcattaaaa tgtcatgcta ccatagtcca taccgtgcca tctgtttaga
    16801 ttaatgacgc tcaatttgtt gaaggattgc tggagagtct tggaatgtta tcatgggttc
    16861 tggctatgca atccctcctg taatcaaaat gtctgaccta cgatacgtgt tcgtcagctg
    16921 gagtcgagtc gaaatcgtat gtgccaaatc ccgcccttat gcgcttgata cttgttatcg
    16981 catcatgttt ttatatttca caatggaata gattgttatg ccaaaggcgt ttgtattaat
    17041 gaatgctgaa ctaggaagtg aagattccat cgtgaacgag ctaaaaaaac ttgaaggagt
    17101 taaggaagtt tatcaagttt atggagtgta tgatatagta gcccaagttg aagcaaacac
    17161 aatggaaaga gtcaaggaaa caataacctg gaaactccgt aaacttaatg gcgtcaaatc
    17221 gaccctgacg atgattgtga tggactaaat tgcataagat taaacgattt ttggttcagc
    17281 acaattttgc accagatttt gaattctaaa taacgtaacg ttaatttaga atgtattggc
    17341 atgacagtta aataatgttt aagatcgact tattcagaag aaaagaagga acgaacgtct
    17401 ttaggtgcaa gtactgtaat ttgcaattcg aggataaaga aaggttgagt agacattcaa
    17461 gaaaagctca cagcgagaag ggtggtgata tcccaaatag gaatccattt ggattttaga
    17521 atactataag acttccgctt aaagactcgt ctgccctaaa aacaacaata tgttccctat
    17581 ccatgatgat gctgagcgta ttcacggtag accatatgta aactatagtc tcatcgcgat
    17641 taacgctgct gtatttacgt gggaggtttt agtaaccggc tttttctcaa atggacggac
    17701 gacctccgaa attttccttg agtatggcgc gatcccaaaa ctcgtccttg taggagacat
    17761 cccaattgtg ctgacatcaa tgttcatcca cgggggaata attcacatcg ctggaaacat
    17821 ggtttttttg tatgtatttg gagacaacgt cgaagataga tttggccata tcaaatatct
    17881 ggcaatctat attttatggg ggttatttgc agctctggtt cacagcattt acgcagttgc
    17941 cgtaggagga ggagaagtcc cagcggtagg cgcgtctggt gcaatttccg gagtcttggg
    18001 tgcatatttg ataatgtttc ctagagcgaa aatatataca ataataattg tgttgttcat
    18061 tacaactatc cgaatacctg cactggcttt cattcctttt tggtttattt tacaaatact
    18121 atttacgttg attggacagt caggaggagg tggagtggcg tatttggcac atattggtgg
    18181 attcatagct ggagttggaa cagggtattc atggaagtat ttagcaggga agaagatgtc
    18241 gtcatctatt ccctccgtgg gcaaaactcg aaagatgaga ccaaaaatag aagacgtctc
    18301 tccttcactc gagcctgagg tcattgaggg tgccgacttc ttcgaaatca tagcagaaat
    18361 ccatggtatc gctgccgcta ccgatatcca tgccgactat gagccggatt ataaacgggt
    18421 tcgcatagtt gcctccggat caaggaaata tgaattgttc gctaaactac ctgattccgc
    18481 gagtaatccg acagttgaat acgtccacta tctcaatgga atcgcgagaa ttcgtctcac
    18541 aaaataattt tgaattttgt tatttgacat tggtatacgt tcaatcaata agaaaagcgt
    18601 ttatggttcg tttcgactca taatgcaccg agatgcctaa gagaatgcct atcagaatcg
    18661 agatcctatc gcatgttgaa agtattcctt cgatacatag agctatggat caaattctca
    18721 aagaggcacc tagatgtaaa aagcgtgtct ctagcttaag aatagattat agttgaagaa
    18781 tagattagaa aaacattgaa tggacaatcg atgaagacga gactgagcat ggctgagatc
    18841 aattgagatt tggacgcaga aaatactgcc cccgcatcgg ctcagccaag ggaaatacca
    18901 ccccccttat actgtaattc aatcaaatcc ggttatgacc agagtaaaat attataagca
    18961 tactttattc taattacatt gtattggagg atcctctgcg ctatagaatt aatcagatca
    19021 ccgatagtgg gatcaatgta tctcttacgc ttagcgaaga tctggagctt gaatcggtat
    19081 cccaacagca gttgatgtta gagtctatcg atagagtgat tactgataaa gaggtgaaag
    19141 accaggtaag accgatctta gaagcaattt taagctccca gccacagact ctagtgaaaa
    19201 cctatgcaga gacgatcgta caaatcacaa tgccgaggag gcgatacgag aagattggaa
    19261 gcccgagggt gggtgaacaa ctcttaattg acataaggaa gccctagatt attacgaggt
    19321 ctctatctcg ctcgggttct ctttcattaa ccagatttga tttgttcctg caagccgctc
    19381 agagaataga taggcacaga acacgtagag tttctacata taacagcata gtcagtttgg
    19441 gtagttcttg ctaaattcgt tcccctaaag aaaggatatt cttgtagctg acttaattga
    19501 gattgatttt caatgatgac agttatcgcg tttggcagga actgtttgct tatccaactc
    19561 gccatctttt gcccttcacc aatattgtct gttattctta aggttatttc gataggcttc
    19621 tttacatgca aatagatagc catcaataac tgaccaaatc caaagggatt ctctgccgat
    19681 gatgtcgcac ctgccttcat aatctgctct gctttttcca ggtaatcatt cttttgagtc
    19741 agatggtata acctcagcag gtcagatgcg gctaccgaat taccgcttgg aatggcgaga
    19801 tcatacaagc tcttagtcct gacaattaat tgctcatggt catccgaggt aaaataaaga
    19861 ctagagtcga tagggtccca aaagtgttga agtatagagt ccatgtattt tattgccttt
    19921 tcgagataaa tgggatttga gtcaagttcg aaaacatcta gcagtgcatt gacatagaat
    19981 gcataatcat caagatatgc attaagttta gaaattccat ccttaaatgt tctatataat
    20041 ctgccattat ctctcgtaac cctagattct atgaattcaa tcgtgttctt ggcgatctca
    20101 agataaagtt tgttattagt aactcggaat ccttttgcaa aaccagatat catcaatccg
    20161 ttccaagcgg ttaatatctt gtcatctcga ccaggtttta tccttttctc tcttatttca
    20221 aaaagtctag acgaagcatc ggtaataatt cgttttaaca tctcaggcgg acgactatac
    20281 ctttgtgata agtcggcaaa cgacgatcta atgttgagta tgttcttgcc ttcgaaattt
    20341 cctgcctgtc caaccccgta atattcgcag aatatactgg caattgtctc atcgccgagg
    20401 gagttaaaaa tttcttgttt tgaccacacg taaaatttgc cttcttcgcc ttctgaatct
    20461 gcatcttggg cggagtaaaa accgttctct ggagacatca tttcacgcct tacatactcc
    20521 agagttttct ctactacatc taaatacttt tcttcaccac taatttgata gagctctgcg
    20581 tatacttgta caagcagtgc attatcgtaa agcatctttt caaaatgagg cacaagccat
    20641 ttctgatctg tcgaataccg tgcgaaccca ccaccgatct gatcatgaat tccacctgcc
    20701 gccattttat ccgctgtaaa catgacgaaa tctttgtacc gattaaggcc agatatatcg
    20761 tagcacctta acatgaacaa aagattggac gggttcggaa attttggcgc ttgtccgaag
    20821 ccaccataaa ctggatcgct catatgtagc aacccaactg ccgcctcgtc cagaattgaa
    20881 cgatcaagga taacaccgtg gggttgttga acatcttttg cagaatgttg cagcgcttgt
    20941 aaaaactcca atgtggcaga atcaatctct tgcttcttgg tcttgtgggc ttcggcaagc
    21001 tgacttaaaa tagtgccaaa accgggcatt ccatgcctgc cgtatttggg aaaatatgtg
    21061 cctacataga acggtttctg atcaggcgtc agaaatacag aaagcggcca accacccgtc
    21121 ccgattgcaa gttggctaac cctttgatat atgtcgtcta tatcgggcct ttcttctcta
    21181 tccactttta tgttaataaa attctcattc attatctttg caatttcctc gtcctcaaag
    21241 gattcatgag ccatcacgtg gcaccaatgg cacgcgctat atccaacgct aaggaagatt
    21301 ggcctattct cactttttgc tttttgaagg gactcttctc cccatggata ccactcgaca
    21361 ggattatgtg catgctgaag aagataaggg ctgctctcac caaatagcct attagcccta
    21421 gctttagttt ccctgtcgtc gccgtgtaac tgcattacaa taacaggtta gtaataatgg
    21481 tatttattgc tattattgct aggtttaata atttacatac aatcaggtaa tgacaagcac
    21541 gggttaccaa gttagtataa ttactaaaaa aattacgaac ctgacgtagg ccctccactt
    21601 aagcattgtt atgagttgcc ggcgagctgt tatcaacgaa tataatcggc ccaatcaaat
    21661 gtcagtatga gcatgctcga tagcgtactt gattagcttt gttcccgcac agccctgtaa
    21721 tgctcgatgg gcattggtct agtttgccct tttaatccac cccctattag ccgaaattta
    21781 tccgtagtac aggctcttac cggtgagcgc tgagctgtac aaggaacaac ccaccttgga
    21841 accatcgaaa tactcacccc aacagtataa cgagcaccta acacaaccga atatagatca
    21901 aggattttgt tgcacggttc tcaacggaat cgtattgaac caccgagttt tatctgcatc
    21961 aaatgtcgct aacgatactt tcgtaaaata tgcctgccaa ttggtgagtc gtcttatgaa
    22021 ttgttgctgt taggtttgat gtaaatctgg gccagactga ctgttgaatt gcgtagcctt
    22081 ccttatcatc gtctgcaatg tttgtgaatc gaaattccat acatacgatt tgccagagat
    22141 gactggccga tgtcacacgt atatcgctgt ccaacttcac tcaataacct cccgatattc
    22201 aattgagacc gccatcgctt ttattcagat atgagattat acgatacatc tgggcatatg
    22261 acaactctag actttgttgt tcttgataaa aagattttaa tttttcgagc gttgatttca
    22321 tcttaaattg attatgcccc gatagtagtc tctccagttt ctttgcttca aaaagatatt
    22381 ttcgaatctg tttctgaact gttgaattct gggagaggag cgctagaatc aaatcaggtt
    22441 catcaagcaa aatacttgtg cacagaagtg actggattct gaaagtcgtc ccagagaact
    22501 gttttaatga ctggtagtct tgcttcgaaa caaaagttgc gaatactaaa tttgtgaagt
    22561 acgtcaatcc tagtatgatc gacataatcc tgtcatgagt ctccgcatca tggataatac
    22621 ttgccacgga accctcgaac agttctttta atattcgtag ttcaatggtt tcgttcctca
    22681 caggtattaa tattattttc attagattaa gactgggtgc tgctgggccg aacataggat
    22741 ggatgcacaa aggtttaaca tgattaggaa ctcttttgag cgctttataa gaccgatttt
    22801 ttattgacga tatttctaca agtatggcgc caggtttcat ttttgtggca cattcttgaa
    22861 ttatatgagg gacctccgag atcggtacgc agaccataac gaggtcagct gtatcaacgc
    22921 aatggacgat gtcttcacat accgttgtgt tggcggaacc ctttagagaa taggcgttaa
    22981 tatcatagag taggagtgtt gtcccttttc gtagagagaa gtatttcgag aaccaggtac
    23041 ccattctacc cccagcgcct atgatagcta ttttctttat catatagtat tagcacgaag
    23101 aaattgcagc ctttaacaat tccagtcctt tctccagctg gtcgtcgggc tggcatgcag
    23161 aaattctaat aaattgagtg tagcaatctc caaaaccact tcctggtgca acggcaactc
    23221 ccagatcaag ggccttttgt acaaattcta cgtcactttt cccaatgcca agttctggat
    23281 agatatacat tgcaccatca ggcctcacaa aacgaagaga catttctcgt aatttatcgg
    23341 agattatttc caatcgtctc tttatgcgtt tcgcattctc agcagaacta tctgctatcg
    23401 cagccagcgc agcgtactgc atcggttcgg caacactggt aagagctgtt gcttgaactt
    23461 tagccatttt atcaattaaa ttttcttctg caatagcgta cccaactcta aaccctgtca
    23521 tcgcatggcc ctttgaaaac gaggatatta cgatactctt ctcgtaccca tattccaaga
    23581 cactagtgaa ttcagtaaag acatagtcac gatatacttc atcgcttagg acgtatatcc
    23641 tgtgatcctt cgcaagggac acaattttct ccatgatatt tttattcaga attcttccgg
    23701 taggattatt tgggtagttc aaagcaatca ttttagtatt gccgttgatc atgctctcaa
    23761 gtttttttat atttggactc cattcctcat ctagggttgt ttttaagatg ttcgtagttg
    23821 aaccaacaaa cgcggcacat tccctgtaag caggccacgc cggctctata cagatcagtt
    23881 catcgcctgc agttaataaa gaggttattg cagcaaaaac ggcgaaccta ccgcctggtg
    23941 taactatcac tttatcagtt gttacatgat tgccgattgt ttgtgctata gacgtacgta
    24001 atttggttat acctttcgtt tcgctatagt ggtattgtct ggattcaaaa acgccggaaa
    24061 gagcctttcc tacactctct gggggtggat aatcaggttc tccaacttcc atgtggatga
    24121 tttttttccc cgaggattcg agcttttttg ccttcatgaa tacagcaaga tgactctcga
    24181 attgaattga gtcatgatgt tcattcaact gtaatctcag ggactctgct aataagatgt
    24241 ttaagagtct cccagcgaaa tctgggtcaa ttccaatttc gttagatagt tttgagatga
    24301 aagttcggat atcctgttca actttttcat ccttgacatc aatctttagc ttttgtttga
    24361 tagcgccgat ttgtttagag agttcgtttc tcctttgcac ttttcgtatg atctcggaag
    24421 taatatcgcg tatttctgat cgaatcctat ctaattcttc cggaatgcta atcttcggtc
    24481 acctatttag taccggtggg ataaatccgc ctctaagagc atgatcagtc aataccattg
    24541 atacgacgca ttctattatt ggaggtgctc tcggaacaac acatggatca tgtctccctg
    24601 gaacattgag gtctatttgt gcgccagtca aaagatcgac agttctctga gttttggcaa
    24661 tcgatgacgc tggcttgaaa ccgatcctta ttacaagagg catcccactt gataaacctc
    24721 ctaatatacc accagagtta ttagtagtgg taattatcct gccatcttgt ttagtatagc
    24781 agtcgttatt ttccgatcct cggcgcttag taccattgaa accggaccca aattccactg
    24841 ccttgactgc tggaacgctg aagagagcct tgctgaggtc agattctaaa gatgcaaata
    24901 ttggctctcc cagccctaca ggcagccctg tactgataca ctctataatg cccccaagtg
    24961 agtcaccgtc tctcttcgct tccatgattg cggctttcat tttttctgct ttcgcggtgt
    25021 cggggcatct tacgtcgttt gtgtatctgt tctttttggc ctccaccaaa tcaatcgagc
    25081 ctgccgtgat gccgccgatc tcctttgtat aagctgtcgt ctcaatcccg agtgaatatt
    25141 ggagtaattt ctgagctacc gaacctgcca ttaccaaagt agcagttaac ctgcctgaaa
    25201 atctcccacc tcccctataa tcagcgaaac ccccgtattt taccatcgcc tgataatcag
    25261 cgtgtccagg tcgaggtttt gttttgatta attcataagg ccctgaatct tgatcctgat
    25321 tccatattag catacaaatt ggcgcaccgc tcgtaaatcc attatacatt ccagatatga
    25381 tctcaacctt gtcctcttct tttctctggg tcgtgatcac agatgtaccg ggtcttcggc
    25441 ggtcgagctg cgactgaata tccgactcat gaagaggcag acccgctggg catccatcca
    25501 ccaccattcc cacacattta ccgtgactct cgccgaaact catcgtaaca aaacgttcgc
    25561 cgagtatatt tcctggcatc tactgtagtc aggcaatctt attcatgttc cctatatatt
    25621 ggctttttaa attaaatttc tgatgtagaa ctctggcgct ccagtccaag tatatacaga
    25681 aaattctggt cagcgggtct agtgcaagtt attatcattc acgatccgcc gtcggtttaa
    25741 tgttacctcc gagttccata acgtcttgaa taaagttcgg atatgataca tcaactgatt
    25801 caacaccggc aacagtagat ttttttgtca gcatcgcgac aatagtaaat gccataaaca
    25861 gcctatgatc gttgaaagat tctagcgatg catttttcaa atttttagga gcaattatta
    25921 gtaattcatt ttttccttcc ttaatcgttg cccccaattt tgttaactgc gaggcgatgt
    25981 ttgcgactct gtctgtttct ttcaatcttg catgtccgat cccggttatt ttgactggag
    26041 atcttgcctt cagagctagt acagatacaa ccggcaaaag atctggagta tcagaaaggt
    26101 caaattcccc gccgcacaac tgttctgtac catacactcg aacttcacct ttttgtctgt
    26161 taacttttat tcttccatcc attgtcctaa taatgtccaa tatgtctgaa tcagcttgag
    26221 gaagacagaa gtttatccca gttattttca gttcatcacc tagcagaagt cctgcagtaa
    26281 gaattaatgc ggcgcttgaa agatcgctgg gaacttcaaa aacggtcggt ttatatgatg
    26341 cagaggggag gtgatattct ctaaaaccct cgttatgcac tgtaacacca aatgctttca
    26401 tggttgcaag cgtcgactca atatacggtt ttgaaacttg tccccccttg attcttattt
    26461 caacagtaga atcggcatat acgcacgaga ttagaagaga cgagacaaac tggcttgaca
    26521 cacttccgtc tacatcagtt gttcctccct ttatcccgcc acctttaact atgagcggag
    26581 gggtcccatc gattttggtg gaatacgctt gtacccccaa tttgctgaga gaatccaaca
    26641 aaggctgcat gggccgctta cgaagacttt cgtcaccagt aagaaccgtg agtccgttct
    26701 ttactagagc agacatcgtg gtcattagtc ttatcgtcgt accggaattc tctgcgttga
    26761 ctacattttc tggcactgta aaactatgtc tgccttttat atgtaccaca aaattatcct
    26821 gtcttgttac gactccgagg gccttgcaag ctgaaatagt tgcaagtgtg tcgcgagaaa
    26881 acagaggatt tttgatagta gactttccgt cagcaagcga cgcgatagca attgccctat
    26941 gactgtagct cttacttgat ggacatctta taatgccatt aatactggat ttcgtgatca
    27001 gaattctaac caactaatgt ttccgccgtt gccttttcat tgttgatttt ggaaacaatc
    27061 acgctgccgt taaacttggc aaaagccgcc ttaatatctt ctatattttc ttcattcgat
    27121 accgcagcaa tagccgggcc attgcccgag atgcttgcag ccaatgcccc tccttgtatt
    27181 gcttttagaa caggctcgta ttctgcagat aagctggtgg aaagaagcat accgttcaga
    27241 ttcatggcct tccaatattc gccggccgtc gcaaacttga aggccgcccc gaacaaatct
    27301 gacattgagt ctaccttgct catattacct cttcgtgtgg ctttaggaat gaagattatc
    27361 gcataaaggt tgggaggagc ctcttgatga tgtattattt cgtgtgtgcg gttattcgtt
    27421 actacgaacc caccaaaata acaggcagaa gagtcatcgt aagctcctgt tatagtcact
    27481 cccgcatcca gtgatgcaag aacggcacga tctaacacaa catagtcttc tatatcctct
    27541 ttaacaagtt tgctacaagc caatgcaaca gcgtcagaaa cagcgctaga acttttcaat
    27601 ccgaatccca taggaatttc tgactcgaca cttatgtaaa ctgagttctc cgcaaggatc
    27661 tcctttggta ttgtgttgta aactatattg cgtataagtt tctcgttaac tgtttgagat
    27721 cgaatgcctt ccccggtcct caaatccgcc tctgctgtaa ctctaagaga gattcctagt
    27781 gcagagccgc tgcaagtcgc aagtgcattt acgatagaaa ttgcgccgtg cactgttgcg
    27841 cgtactcttt tttttatcac aagggttcac cgaaaggacc gaagagagct tttttcattg
    27901 cttccaccgg cgcagttatc cctgtccaga tttcgaacgc tttcgaccct tgcgccagga
    27961 gcatctcata tccataaatg actgtcgctt ttgcattctt tgcctgtgca attaaatttg
    28021 ttaccattgg tgagtacaca atgtcgtaaa caatgctgtc tttacttatc tgctcataat
    28081 ccacaatact ttgctcatca ttcatcccac taggagtgga attaataatt aaatctgact
    28141 tgagcgcatg ttctcctaaa tactccatcc tagtaaagtt agcacttaat ccaagattag
    28201 aagccattgt tattatttca tccgcctttt tctttgtcct atttgcgatt attatgtggg
    28261 agattccttt ttcattcgag agggcagcaa ttattgctcg tgctgccccc ccagatccta
    28321 tcagaagaat tttcattccg ttaaagctta cacgccgttt gtgcagaggt tctatgaagc
    28381 cttggacatc cgtgttatag cccttgaaag ttccttcaat gcagtgaact gtattaactg
    28441 cgttagcttt cttggcagaa ggatcaagtt cgtcgatata cggtatgata ccaaccttat
    28501 gcggtattgt aacattgaac ccagatatgt tggtcgcacg aagagaacca agagacacct
    28561 cgagctcgcc tttgggtact cgaaatgcaa tataggcaca gttgaggcca actgacttga
    28621 acgccgcatt gtgcattgca ggtgataatg aatggtcaat ggggtctccg atgatacaat
    28681 acgttttaaa taagtcttgc attgattttg cctgagcaga gatcaatcct atcatataac
    28741 tttctcattt gttgtaccgt cagttgacct ggggcaactg cttcttctag ggcagcatag
    28801 gtaaagggcg catttccata gatcgtacat agaatacgag ataaaattcc tagttccccc
    28861 attgcgaatg atattaagtt tagttcggtt gtttcgtcat acagatcgag aattcgtatg
    28921 cagtcgtgaa cattttgggc agcggtagcg atcttcaagt aattgctgta gatcctcatt
    28981 tgccctaaaa catct
//
LOCUS       DAOR01000211            5630 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_51649, whole genome shotgun sequence.
ACCESSION   DAOR01000211 DAOR01000000
VERSION     DAOR01000211.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_51649"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5189..5481
                     /estimated_length=293
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1688 a         1023 c         1018 g         1608 t
ORIGIN      
        1 ttgcgttact tgctagctag aaggcgggca attatccata gcaaaagaaa aaccgtttat
       61 acttaaagaa aagggtcacc tttttggata ttacaacctc ttccacttgg aagacacgat
      121 attagcttta aggcaatagc cggctaggtt acgtctacaa gcacccaaac tttcgtcaat
      181 ggtgtcacct atcacctgac agtgcagtaa atcgttagca taaatcgtta tcaactttga
      241 tctttccatt tctaacctct gttaacacct cttgattcta gattctttca attatcgttg
      301 tttatattgt agtaaggaag aattaaataa tcttttatcg aaaacgtaga ctatgcaatg
      361 cgcagtttat gatacatatg tagagaaaaa ggacgggaag actatgcatt ttgatgttat
      421 tgtcgaagaa aatacacaac aagaaaaggc aatagagtat ggtaagcagt atcttgagag
      481 cgctggtcag ggtggtcaga gaatgactca ggaagaatgc caattttgcc atatccagga
      541 agctcaacct gtagttgaaa gggacattac atcaagaggt tattatatac aaaaaatgga
      601 aggctgcaca taatgaatcg atgtttattt ttttaaattt agtggctgca aaaaccgaac
      661 acatccttga taaatgaaat taccgttatc atttacaatt ttcttgatgt gataggccta
      721 tcatcagatc gcacaatctt cgggaaaggc atattagaca tttctaatta gtatttctga
      781 tatgaagttt caaggattcg gtatacaaat cataatcata tcggttcttg gaattagtct
      841 gcttgcagct ggtcttgctg ctacctcagc gtatgccttc gtgaaaatta cttcacctgc
      901 caaaggtcag cacgttcctg taggcaacat cctaatctcc ggaacatcct catctaacgc
      961 aactaaccat tgtgccgttt cagtcattat taacggaata aaaccatacc agcaagcgac
     1021 atcaacagga aataaggggc caaatgacta ttcaaattgg acctttgccg ctactccaaa
     1081 gtatggcacg attaaagaag gagtaaacaa gatgactgca aaatactctt gtccacagaa
     1141 tatcaatttg acgaaatttt atagcgtaaa cgtgacagga gtggctgcca aaggacaact
     1201 acaacctata ccgccaacac ctgctaataa tgccaagact acaggcggtg catttccacc
     1261 gtctcttcct agtgtaaacc cgtcaatagt aaatgagtcc aagcacagta agatcctgaa
     1321 gggctccaag tagtgaggtt ctgtcaaatc ttcttaagat atcacaattg tcgaaaagca
     1381 ggacataaaa tctccaggaa gttgccacga ctaagactat caaaccataa tggtagtatt
     1441 gcagccaaat actttgccag acagtattcc agttgaaaat tatgttaacg taacttgtga
     1501 attcagacta tagttatcac gaattgtttc atattggttc aataatcgat ggttttagcc
     1561 cacaagaaat caaaagaagt cgaaatataa gtgatacctc ataaaggttg ctgcgattct
     1621 tgcatctggt cttcgaaatt gtatctgatc cgtgttaaag taaccaagac attagcatca
     1681 tttttaaatt tcaatgtcaa tcttaattcg tcatgtgtaa ccaaatgtat ttcacaactt
     1741 gtattgtctg ctgcattgta tacgtgccaa tcttgtgact tgcccccaaa gttattcatt
     1801 cattattgat gctagaaaca aaagtgaaat aaacgaaata aacgatgagt tttgtttagc
     1861 acgctgacat tctaggaact gtagttccaa gttaaaggct aactttcgtt tggtttagtt
     1921 gatatgcatt gctaccacgt ggagaacttt ctctcttctc ttctttctct atccttgatt
     1981 tagtcacgtc atcttcttct ttattgtcat gaccgattgt gagtgtaagt agcttacctg
     2041 acgctgttct aatttgttgg attatgtctg caccggaagt tcccaataca tcgacgatgc
     2101 ttgttattaa ttcgttgttt gtaagtgcag gagcattctc ctaaggatga tgtttaacca
     2161 tcattccagg agaattcgcc atcatacctt ggtttattcc agaacggaaa ctgcagcagc
     2221 caaccgacca aacagggctt aattccacaa tacccgtaac aattcctcgt ttgcgtcggt
     2281 tctcatcgcc tcttggtatc gcacagtaaa gaaactgatt taaggtatga atgcgatgtt
     2341 aatgtatgtg cgacttcatc ggttatgaac tcaaagataa gtggcgggtg aagaagattg
     2401 tcgaagaaga cttagaagaa gagcaaccag agcttgagaa ggtacaagaa caacctctta
     2461 ccgtcacaca ataggcaaaa agacactagt actgaacgtg aacgagtacc tttagtcaca
     2521 ttcgcgttta ctcagcatta gttattagag aatagatgaa actcgacggt tttagcggct
     2581 aaaaattcag gactgaaacg ggcttaacac agcatcctaa cctggaacgc cagtccagcc
     2641 gcgcttatct ggagtatctg ggcatgatgt ttattgcacg cttcatctac cacgatagcc
     2701 ccactaacag acgtatagca tcatctaatt ccatattctc tagctgctag ctttgaaagc
     2761 gggaattttt gtcattaatt actagcattg ataatgtaac aagttatgat tgcagggttt
     2821 cagtacttcg atattagcat tagataatct tcttctatcc tttcctcagt ttgaattcta
     2881 attttattcc tattttctcg ttgtaattcg aggtgaatga ttgattgggt aatatcggaa
     2941 agcttgaaag ttctgtattt tgataattag ggcacctatc ttctgctggc agcataagct
     3001 cagtaaagta tgttgcagac caataacacg tatcacatag aatgaatatg gcctcttttt
     3061 cttttgtgtt tggataaatc tgtttctttg tcctgggggc tgttccatat cctttgctat
     3121 tggtcaatct ttgaattgcc agtattcgac gctaaactat tatattaatt attctaggtt
     3181 gtcttgccac attcaatcct ctgctatatc aagaataaaa atgatctaat attgtttacg
     3241 ttttacaacc tgttacaaag gggctagagt attataaacc aaatgtgtgg taggatgaat
     3301 tataccagaa attggtgaca ccttgactga aaaaattagc tatattctag ccaactctga
     3361 attcagagta ctaatcttaa gcaagaattt cttcagaaag aattggtcca gagatcagct
     3421 aaataggtta tcgacaaaat cgatattgac tggcattcta actaatacct tgggaagagg
     3481 ttgaatacat caccaaaatt gttccatctg cgttggtgga tcaaaattcc acccgtctca
     3541 aataagttaa gataatgtaa tatttgttaa agctgtctcg ataatctcaa taactcgcta
     3601 gtggcatgat attccgtttt gatcataatc attgtatgaa tctgcgattt gcataaatgt
     3661 tttctttaaa gtatcaatat ttgaggctat gccttttggt gcattttctg tccatgcaac
     3721 cacccacagt gcatcgtgag cggtatgact attgtacgat atagagcctg cccctagaat
     3781 tagaccatat ttcgtcagcc aagatagcca aatccggtat tttgatgtct acccttacaa
     3841 tcttaataaa acatataaat atatagattt gtaagggtag gttctgtggt aaccataaag
     3901 aagagaagag taggcactgg tacttactac tatctccaac atagcataag gaaagggcca
     3961 aaagtgcaga gtaaggagat ttacttagga aggaaaatac cgaagaatat cgaagaaatc
     4021 aagggaaaac tgctcaagac catttatgag aaggagtggt atcctctctt agatcgaata
     4081 aaagaaaaat attcgaaaga aatcaaactg atgcccagat cctccagaga gaagcagatg
     4141 gagatcttcg caagcagatt tacctacaat acgcaaagaa tagagggctc taagctaacc
     4201 ctgaaagaaa ctgccaattt gcttgagaaa ggcataacac caaaagacaa gccgctggaa
     4261 gacgtcaaag aggcagaggc acacaagagg ttgttctatg ccgaggtatt aattagcaaa
     4321 aaaaaggatc tgtccctcaa tctcgttgtt gaatggcata ggaatctatt tcaagagacc
     4381 aagccagata ttgcaggcaa gattagaaag catcaaacac gtatctcagg tagtaaattc
     4441 atccctccac ttcctgttga agtttatccg cttcttcgag aattcttcaa atggtatgat
     4501 aggaataagg gtaagattga actccatcat cccgtggagc tggcagcttt tgttcacttg
     4561 aaattcgtga ccatacatcc ctttggagat ggaaatggca ggatctccag actgatgatg
     4621 aattttgtat taaacaagga aggttatcct atgctcgata ttccttatag tggtagaaac
     4681 agctactaca ctgctctaga aagagcgcag gttaagaagg tagacagggc attcattcaa
     4741 tggtttatga gaaagtacat gaaagaaaac gatgcatata gttgaacata gcaaaaattt
     4801 tctacaccgc gtttgaggaa gtcataggta gaaaataaag aagttgtgcc atgaccgtat
     4861 acgtaggagc tcacatctgt atttaacgtc tgtgaactac cactgattaa aatcagtggc
     4921 ttcctttctc atcattgcgt tcttgtctac gctaggaaaa gtcctgggta ttttcacggt
     4981 tacccctact ttagaaatat atcctaaagc ccgataggca tgtaatatct catattcatt
     5041 caaccgattc ttttctccga agaaaacaag gcatttacaa acaactattt acagaaattt
     5101 ccagtaaatg ttactctttg atttaagata atttttcaat tattttagct gcttcagaca
     5161 ttggaaatgc tttaagtgtt ttagtacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn ngctacaatg tcatgctttc cgagtgtata atacaagtta
     5521 atgactttcc cgcctttctt ttcaactgca ctcttaaaag cttcagctct ctttggacta
     5581 tcttttacgt tcttgattcc ttgatctgta aaattgacta ggataatata
//
LOCUS       DAOR01000212            7489 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_23498, whole genome shotgun sequence.
ACCESSION   DAOR01000212 DAOR01000000
VERSION     DAOR01000212.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_23498"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    428..593
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1911..2151
                     /estimated_length=241
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2059 a         1424 c         1361 g         2238 t
ORIGIN      
        1 ctcagttatg caacatagca gttctgagct ataaccatat tcacaagagt taatcgaagg
       61 taatggcata gttcccatct atcttttcct gcttccttaa tctgttattg tcgacgtagc
      121 aagaccatcc acttttggtc cgttagccaa agcttttagt taaagagtaa gctgttacaa
      181 ctaggatgtg tgcaagaagg taaacagggg tttgtatcta gtggcataaa gcatgttata
      241 ggatgatatt tgaaaataaa gatggtataa taaaatacga caaacgtgag ttgtacgagt
      301 tcgacgatct ggttagttac ttttaactgg tatcgttacc atttaattag ttaccgtact
      361 ctcattctat actagacggt aatgtcaaaa tctgtaagcg aaaataccat gttatttaag
      421 ccactctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcagga
      601 acaatagttg tagcttttgg tgtaatctgt atctggatag ggacgagtgt cgtatcacct
      661 aaagaagacg gtgaaggctt taagatcatc ggcattagat ttctaattgg tggaatggtt
      721 tcaattactc tggctttcat agcaaaatat tttacgctta ttaaatatct tttaaagcag
      781 atcagttaca aaggaaattc agaaggagac aagttttctg attggcataa gccctgacga
      841 ctgtgtgtta gagcacgaat agatccgatt agagctcaat ttagtagttt ctattgcagc
      901 gatgcagaga taatcgaagc aaaaggaatt caaatttctg aatcctttta ataacctaag
      961 aatcagaacg atagtactac aaatcgatgg caatgttgct gtcagttagt tggatagtgg
     1021 acaacaagtt gttttcaaag caaagtggcg agctaataaa ccaacaggtg gcagcgggat
     1081 ttgggtggat actattggcg atgtaactga aagcaatggt tttgtccgtg acgacgaagg
     1141 tagtcaatta ggcaaagata aagaaacaaa ggcgagggga ttgtttattt taccgctttc
     1201 gtcttcaaat gacccacgtg aaggatgggt ggacttcgga tctggagcag ttgtctacaa
     1261 tgtatctcat ccattcgcga aaaaccttga tggacggttt ggtattgcag ggatcgtatt
     1321 ggtaatttaa atactaaact gtcttcattg cctattcttc gtcctgcgtc attgtgtgat
     1381 ttgttgccaa aatcaatata cccatgagta cagtcgctgt tatcatttca aatctcattt
     1441 atctatttca tcatcctgat tgtcataggc tgcattcctc atgagttcaa tatccagtcc
     1501 attagaatca gtacatatct cttctgtacg cttgtaggta gatatgggct gtaaatattg
     1561 ttgattataa atatataaaa taatattgca gtattaaaaa tatttgactc ccgctaacgg
     1621 cagcgcggga taagggatac ggctctagta ttatatctaa atattattat tagatttatg
     1681 catcgacaaa aatcgagtaa ttggttgttt ggtagttagt cagccaatac gctaatggtt
     1741 atcatcatag tagtcactta cattcgtatc ccttgatagt agcaggcctg ctttcatact
     1801 cttgatagaa gccctgtttt atgttctcag ctaggctgct gatttccttt aaatcattga
     1861 attcatttgt tttctttaca cctacaccta atgactccaa ttcatttatc nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaccaaccg
     2161 tatctttctg gatgttcgtc ttttgaggcc acacaaacac cgtcttacat tattccagaa
     2221 aattatacaa taaaatcttt tctgaatcat ttttagtatc actaaaacag gctttttgca
     2281 ttctattgct atgccatgtg cggcttcaga aatgcttcgt agtaaaatca gccttgacag
     2341 catagttgac tataccttcg ctatttttaa tctgtttcaa cacctttaat gtcaactctt
     2401 tagtggcaga aatcttgcaa cagatatgga gtctcttacc gcatttacat ttgtgtcctc
     2461 tgcattatag ctatctatat gaaatttctt aaacttattt cttgggtaag ccataatatg
     2521 aagtatgatt tcagactgtg gtttttgtaa acttacatat ttggtccgtc tattcacatt
     2581 ttaatgaagg attttttcaa agattccaac tctaaaaaat tagctatgtt gagtataatc
     2641 tcggtaacta cattgttgat gatattgtct ggaacagcaa gttttgtttc aattaactat
     2701 cataccgtat atgcggctgc aaagaatgca acaaagacca cctcgtcatc atcgacagga
     2761 gctaacccgt ctggcattag cggtagcagc gctggcggta gtagtggtag caatgctgga
     2821 ggaggtactg gaagtaataa caatagccca ggaccgtcct cccgacaaga aagcactgcg
     2881 cattgtgata ggcctggatt cccatcctgt gctagtttgg gctcccaagc tggaaagagc
     2941 gctcctggaa ccaactgtcc tcctggtcat agccaggcat tttgtaatgc atatacggca
     3001 gctgctggag gtccaactat taataataac aatccagggg tgtcttctca acaagaagct
     3061 gcacattgcg atcagcctgg atatccatcc tgctacagtt taggctacca atctggaaag
     3121 aaccatcttg gcactagttg tcccggtggt cacagcgcaa aatattgtgt tgggtggaat
     3181 gcaggagcag gaaatacgac atattgcgat caaccggcat ggcctagctg ttatacttta
     3241 ggctatcaag ccggaacgaa tgccctcggg actagttgtc caacaggtca tagccaggca
     3301 ttttgtaacg gatatgaata cggaagtggt ggtaatggaa gagcgtatgt acagggagtt
     3361 actgatccca cacattgtgg tcagcccgga tttccagctt gctatgacat aggaaatcat
     3421 gatggataca ataatgcagt aaatgaacac caccaattca ataattcatg tcccttagga
     3481 cattctaaga cgttctgtaa tggatatgta cctgggtaca accgaggagt atcatacaat
     3541 ctaggttatt cacggggtgt caacaattct aatcatgatt gggagtcaag tagtcaagat
     3601 atacatgcat ttaactacga ttgcccaagc acacattcaa aggatttctg taatggatat
     3661 acggacgggt atagtgacga agtgcatgat atactagggt aagtccttct ctattactcg
     3721 caatagtaaa acgacactta agaccagtct aatgtctttc atgatttacc gcacttattg
     3781 attatcagct ggttgtacca atttgacata tagagctaca gcaatgtcca ctcagatata
     3841 ttgagattct ccaagaatat aggatctcct tactgggaac aaaatgacaa aagattacag
     3901 taactgtctc attaaatatt tcaactactc gtcgaacata aatcaaaaag taggcgagct
     3961 aaggagcaac cacgtaaatt tcagctgcga tcttttgggc ctcttcataa attatctttg
     4021 ctaagtatcc ctctatccgt aatacagcgg catctggacc cttcgacgcg atagactgta
     4081 cagtgaaccc aggcaacgat tctttcatca ctttcaaatc tatattcata tgttcaactt
     4141 atcaattcct tgaaaaattc tccataagtt cgtggccagt tgaatcgata tgctcctccg
     4201 ccatttgcat atcaataaac tccttgttac atggaggcaa tgatacgtta ccttcatgat
     4261 acaaaattgt gcatcaaact agaaaaaagt ttcacacctc catgagacag caattttgca
     4321 agagaacgag actaagaatt aaagtgcgat ttaatcgctg tcttccagat ctgttgttgt
     4381 gtggtcaatt ttgtatctgt ttgaatcgac atagtatgtg ttatccaata agttgtagag
     4441 ttcatccctc gcgttctggt tcttaattat ttctgtctcg agctctctgc ttaacgcctg
     4501 ggtgaataga cttcttagct cgataaatag agagactgac tgtgtaccat tgttattagc
     4561 aacgctgggg ggagaagaca agaaattgca ttaccagttg aagtagtgtt tacttttgtg
     4621 atcgttgagt tctccctgca tattgttggt cttgttggtt gcgatgcgag taagagaagt
     4681 accattgcca ctaatatgtg atattaaatt aggatcttag ctccattcgt cagaggcgta
     4741 gaactaagga gaaataggat tgcttaaaag gtacacctaa gtatagctct caaatcgttt
     4801 cgataagatc tgattacata ctcaattggc aaaaaaatgt ctcaattagt ctcgacaatc
     4861 gatctgtatc atcaaatcat aatattccag tgcaacagat aagatacgca attaaggttc
     4921 cagcactgtc aattttcctt tcgcttacca tctcctgtaa ttttttaaca gataccattt
     4981 caatcgttct tatgtcttcc atggtatcat gtttaggctc ccctttaaac aagttctttg
     5041 ctctaaagat atgaacaagt tgttttgata tgctaactga tggatgatat gcatatagct
     5101 cttcgatctc tttggcaagg taaccagttt cctctaataa ttcacgtcta gctgtttttt
     5161 cagcatcctc ataattctct atatgacctg cgggaaattc aagtaatact ttgtgtaatg
     5221 gatatcgata ttgccttatc ataactaagg tgtctttgtc aagaaacgga acgataacag
     5281 caacatctga agatttatac catgtgtaat gcatcatctt tccggtccct atttcgactt
     5341 tgtcttgata caattcaatc catggatttt ggaagattag gtcgctttgt attgttttcc
     5401 agtctgaggt catgttagtt acttattgtc ttcctttttg gctaaatata ctccttgttt
     5461 gtattgtctt tttgtcaaat tcatattttc ttgtagcgct tgtctttttg tagcctaaag
     5521 gggcgccacg ctatagacaa ctactagatt ttagaaaaga gcacgagaag atcagcacct
     5581 tcacaataag ccgtaagttc aactgtttca atctattcaa atctccgctt cagtcgatat
     5641 atgaatcaaa acggtaattt attcatcaac tgagtatgaa tccgaagtaa acttggtatc
     5701 atccgggagt aatcgatttg ttatctccag tcgtcaatca ggtaaatctc cggtttattg
     5761 taggcaggtt cattcggtct atccatgact acaccttgaa tttcattgcg aaaacctaat
     5821 gatatcattt tgcggtatgc ttcatgcctt ccagtattaa cacctgctac aagacgggac
     5881 acaccttctg ctttcgcaaa tgtttcacaa gcatggacga gcctatcaaa atagatagcg
     5941 gcattggccc ctgggagtac tgcgccaaac ttgatgtagc atgctccgct tccagcctcg
     6001 gtaccagctc cgcaatggca taaagctaag cctaccagcc ttcttctgcc ttcgtcatgt
     6061 cagaggagaa ctgtatcgcc cagactttga gtattaactg cgttgatttc atgttgcaca
     6121 tccaatccat catatatggc ggtagttaag ttacggcacc cattgagaca tcctcttcga
     6181 tcttctgcta ttacctcaga atatctggac cattgcaacg aagtcttctc ctcatctggc
     6241 attgttgctt gatcggcgaa aattgttcca tcaccctcta gatgtttagc cattatcgca
     6301 gtaaggaatc gtggccaaaa gccaaatctt tgatataacc ccagatgctt cggactttgt
     6361 gcccaagtaa acaaaccaat atgcttggta cctaattttt caaaataatc catagttgat
     6421 ttgagcaact gcttggctat tcctttatcc cagagatctg gacgaattgt tactggtccg
     6481 aaaataccaa cgctacccca gttagctaca aaatttgatc ctacaagctc accatcaact
     6541 tcagctgcaa aggacccaga tggattagcc ataaatcgtg tgcgtataaa gtctgcatca
     6601 ccaaagaatg tcaatggatc ttgtaaacca atgaaagtac caaaagccaa tctaaagaca
     6661 tgatcggcct tggaaatatc actttctctg agtggacgta ttagtatatc tgtcaatgcc
     6721 gctttcttct gcgctatgct accctatcct agaatgccgt tttgactatt tttgttttga
     6781 catatgttct ccaatctgtt gaagctcttt aacaaaagta ttgtaatcat aattttgtac
     6841 atgcaatata ttactccaaa aggcgctttc agctacgacc aaagctaatt cgtttaactc
     6901 ttggtctgta gcgccaaaca tttttgcaac ctctctgtga taagtctgac agtatggaca
     6961 ttttgtagca gcggctgacg ccagcatcat catttctctg tactttttag ggattagaga
     7021 ttcgccgatc tggtatttct taaacagcgg ccacatttgt ggaagaacat cttttggtgt
     7081 attcttaaaa aatccaggga gcatgcccaa tgtcttttct acatccttaa cggtctcttc
     7141 ataactcatg atcaatcaga aggacaagca tcctattaag gagttttatt tccaccagta
     7201 ttgctatagt tacatatcca tagcccatgt agcggtatag gaaaagagaa ttctgaaata
     7261 atatagttat ttatcatact cacttatatg actcctcaag agagtaatgt gaattctagt
     7321 cctgattctt tggaaaaata ataaaaccct cagaaattca agcagcatct tctccaaatc
     7381 caatttttca attgccaatt ggttggttgt ttgcatatca aagacactga tgcccgctgt
     7441 tgagctagat gtctttacca aactgtcctg caagtcgata agtgtgaat
//
LOCUS       DAOR01000213            9338 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_8099, whole genome shotgun sequence.
ACCESSION   DAOR01000213 DAOR01000000
VERSION     DAOR01000213.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_8099"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2987 a         1809 c         1976 g         2566 t
ORIGIN      
        1 tgaaaaaatg gagggagttt acttcacaac tacctttccg accattgtgg gatgtagctg
       61 acagaagtat ggaaactccc ctgcagtcat gaacttgtgg gaaaatgttt ttccctgagt
      121 agtcaaggca gtaggcccag ataaaccaga atcaaactcc tttccatggg ttgtatcgct
      181 gggtccagta ccggacgtga ccgtatggaa agtcgtatcc ttgttagtcc aagttactgt
      241 atcaccaact ttggcattta gcggattcgg cgcgaaggct ttgtccgtca tagtagatgc
      301 attaggcact attgaaacac tattaccacc ggttgagtta gtagaggtgg tactgctgct
      361 atttgtctgt tgagctgaca ccaaggcata aggaagactt cccaatgagg ctccgagtgc
      421 aatcaccgca actattccta gaattgctat cgactttttt gtagacatgc gaatcataac
      481 attaaactat tttataagtg gttcatagca ttatattagg tgtttgccca atgattatac
      541 aaattgtgta tggtatatgc tccaatccat ataatttatg aaccaaagaa gcactaagtt
      601 gagattttaa agaccatgac atgtcggttg cgaaaaattc atctcaagtt agccaaagca
      661 gggattcgca tagatcacga cacgatatca atacaaattg aagttctaag cataccaatt
      721 ttatagttca atatcacacc atggtcggat gagacttatc tccactttcg agttcgaatc
      781 ggctaaaacc aggaaaacaa agtcaaccaa aataatgaat tcgctgatgc accgttcgtt
      841 gaatctcgct gccgaatgca actccgcagt gtgtacagag gtatattccc agttgttcaa
      901 tgtccccttt tttcaagaga gtgatctcat tctgcttttc agtattttcc gtgacgacga
      961 aatcctgtat cgtgttttcc tttaagatct cttccaacct gcttttaatt ttctgcgcat
     1021 tactttcgtc ctgtagtata ataatacctt cattatggtc tgggtgatat tttagatcaa
     1081 tcgatttatg aattatatat cttaatctct cgggcatgta tgataaatcg attgaaacga
     1141 ccatggttaa ccagtagtcc agatatcctt taaatccatt cctgtatttt agctagtatg
     1201 agaacttggt tgattctgat aaatgtttgt gaattaacta tgaacggtat actcattata
     1261 cactttcgca ttatctttaa tcgttgtcag tcaatatcaa caatgtcgaa gaatttctta
     1321 cgtcggagtt aaagctgtct aaggaagagt caaaaacgtt tctgtttatt gtgaagaatg
     1381 gcaggaaaca aatggaaaaa atagccgaat cgttgaaatt ctccgaagaa gaggccagca
     1441 agatggcgaa ctctctagtg gagaaaggga tgattatcaa catttctcaa tcagagtatg
     1501 aatcgcttca tcccagattt gctatttcca accgttatcg aagactttgc gaagaagata
     1561 atattccttt cagaaaaaac ctgctgatag ataatattgg gatcgtacta gagctaccat
     1621 tcgaaaatgc aagaactaaa taggtatact gagagccact ctaatatggc atctgtagac
     1681 cccacagatg gagacaaggt cgtttatttt ggtgtgataa acgttaacga aggtggaaga
     1741 actatcggtg ctgttgacat atggcgaagc ctgattacca aggaattatt ttgtgaagaa
     1801 aaaaggttgg gagttttaga gatcgcggat tatctaggta tgcctaagat tgataaagac
     1861 aagaaatggg ctgttacaat aagcagagaa agagaaggcc gtgacagatg gaagttagta
     1921 aaattgatta accaaggtaa gttcaaattc accgatacag acgatgagtc tgttatcgaa
     1981 gttgacgtta atgggtacaa agttattgat agcaagtggt ggagttttct tgttgaaaaa
     2041 aatgtaaatc gtacggtaga tataacaaac gagagcaacg caagatgatt ggacgagatg
     2101 attccgacaa taatatcgtg ctgactgttt ttgttcacaa taccagagga ggcgcaattg
     2161 caagcaagtt gataggccac tttctattaa aagatcaaga atttaaattc acggcaattg
     2221 catttggaag aattggaggc cataatgtta gcctggggct atccaaaaaa gccaaagaaa
     2281 aaattaggaa aatgggtctc gatcctgaca acgtacagct ggctgttcaa cgaaaactaa
     2341 tcgagggtga cgtgattctt cctgaaggtt taaaacttcg ggaatagatt catagtaacg
     2401 atccctctaa cgcttttccg caattacaat ttactctctc tgtgggtatt agcgggatca
     2461 tctccgttat caaattcttt gtcctttcaa cgtttttgtt taacagttct aagatagcct
     2521 ttgaggttac ctgagattca gaccatacgt cataatctgt aatcgctgaa atagagacgt
     2581 agcatatctg cgcttctctt gctagcacac attcaggaac aatagtcatt ccgattatat
     2641 cagcccctaa tacttttctg aacaactgtg actcagctct agtagaaaac cgtggtcctt
     2701 ctatacacac gtatgttact ttaggatgaa ctatattttt taattttgta gcacaattta
     2761 ttgcaatgtg gctaagatcg ccacagaacg gatcagcaac agaaatatgg caaatttctc
     2821 cgccatcata aaaagtgtat tctcggctct tagtaaagtc gataaactgg tctggaatag
     2881 caatttctcc tggcttgtat tctcgtttca gacttcctac tgcagatggg gcaattattc
     2941 ttgtgactcc aagctcccgt aacgcccaaa tattagcgcg gctgttgatt cgatgaggag
     3001 ggattgtatg tcctctaccg tgtcttggaa taaaggcaat tgttcgtcct gcatattcac
     3061 cgatattgat aagatcagat ggttcgccgt acggagtgta aacctttact tgattctcag
     3121 tgccgaaaag ctcagcagta taaatcccag tgcctccgat gattcctatt tcagcagagt
     3181 actttccatt cacggcttaa tcatacaccg ctaacgaata gacatcgtgt cccgtctgcc
     3241 ttaactttgt cgctccctga aggccagcca gctctatcac gaaggcaaaa gctgcaattt
     3301 tgccccctag ttccttcact aattgcgccg cagcctcagc agttcctcct gcggctaaca
     3361 agtcatcgac gattagtaca ctctgaccat ccgatattgc atccctttgg atttccatta
     3421 tcgcagtacc gtattcaatg tcgtatgact ttctgatggt ttcgccgggt agcttaccag
     3481 cttttcgaat catagtcagt cccttcccaa atctcaatgc cagaacagta gcaacgacaa
     3541 accctcgtga ttctatacca actattgtat cgaatttgta actcttcaaa atactctgga
     3601 attcgtctgc aatgtagttc aaagcatcat tattgcggaa gacagggcta atatctctga
     3661 ataagacgcc ttgcttgggg aaatttggac aatccagtat taatttgtgc aaatcgatcc
     3721 gttttgtgac catagggtgt agccatactg ttgccatata aaattacggc gctgaacaat
     3781 cccgtctgag tatataaaga caaaaattct tcgtaaacaa cccgaattca gcatgttgac
     3841 taactcttct actatatttc atgataaaaa agaacatgca gagcttttaa acaaaaaact
     3901 tctaacaaga tataaataat atcattatca ttaatataat tagtatgcct atccgaaatg
     3961 gggcagaata tattcaaagt ctcagaggca gaagtttaag agtttacctg tttggcgaat
     4021 tggtaaagga gcctgtggac catcccttga ttaggccatc tatcaacgcg gttgcagaaa
     4081 cttatgatgt cgcggatcga gactctgaaa ttgcctctgc acagtcatcc attagcggga
     4141 caagagtaaa cagattctta catattgcag aaaacactat ggatttggtt aaccaaaata
     4201 agatgcagag aaagctcggg cagctaacgg ccacctgctt ccaacgatgt gtaggaatgg
     4261 acgcgctaaa ttcattgtat tcgacgacct ttgaaataga cgtcaaatat agcactgtct
     4321 atcacaagcg cttgattgag ttcataaaaa tgctccaaaa ggagaacttt atgataggag
     4381 gtgcgatgac agacgtaaaa ggagatcgca gtctatcacc gtcacaacaa gaggaccctg
     4441 acatgttcgt ccatgtggta aaaagggacg aaaagggcat atacatcaca ggcgccaaag
     4501 cacatcagac aggttgtatt aactctcatt ggattatagt aatgccaacc atgagactta
     4561 gaccagaaga taaagaatat gccattgtcg gtgcgatacc gactgacgca aaaggtatta
     4621 cctacattta cggcaggcaa tcatgcgata caagaagtat ggaagacggg gacctagatg
     4681 caggcaacgc caaattctct ggccaggaag cgatgatcat tctcgacaac gtattcatcc
     4741 ctaacgaatt aatttttatg caaggagaag ttgaatttgc gtccatgctc gttgaacgat
     4801 tcacatgtta tcatagaaga agctatgtat gcaaaaccgg tcttggagat gttctaatcg
     4861 gtgcggcagc tgccatcgcg gattataatg gagtggcgaa cgcatctcac atcaaagaca
     4921 agctagtcga gatgactcac ttaaatgaga caatttttgc tgcaggaatt gcttcttcgt
     4981 accaagcaca caaaaccgct tccggtaatt atcagaatga cgatatgctg gcaaatgttt
     5041 gtaagcacaa tgtcacccgg tttccatacg aaattagcag gctcgcacaa gatattgcgg
     5101 gcggtttgat ggtaacgatg ccatctgaga aggactttag aagtcccgaa accggtcctc
     5161 tattagagaa atacctcaaa gggagaaagg gtgtctctac tgaaaatagg gtaagaatat
     5221 tgcgattaat tgaaaacatg acactcggaa gaaacgcagt aggatatttg actgaatcaa
     5281 tgcatggcgc tggatcacct caggctcagc gaattcaaat tgcacgccag atgcaactcg
     5341 agtacaagaa aagacttgcc aagaacatag ccaacgtaat agagggcgaa gcaaatgacc
     5401 ttacccaaga acagtctgaa tatttcgata gggtttttgc cacgtaatag gcaggaaatt
     5461 aatgtgcatt gcacggctgg ccgacagaac agcattttcg tatagtaatt tagtagctgt
     5521 attgtatatc gagtgcgtgt cgacgtatgt tcaaccatct gtgtcaaata tttaacttgc
     5581 ttactattgt agcttgataa ctcatgagtt ctaacactat tgaagaattc atccatcgct
     5641 catacgtctc gaaaccgaag tacatcgaaa taatggcagg agtccctgaa gattattctc
     5701 aaatccaaac cttaggagac cttttaggaa ttaattacaa gcatgttaca gtagagaggc
     5761 agctgcgtgg caacctcatt acaaaattga aaaaccacga atatccttac cctggtatta
     5821 tcggatataa tgaagatgtt attccggcgg taaacagggc aattctggcc gggcatgata
     5881 ttctttttgt cgggcagatt ggacaagcaa aaactaaaat tgcagaatgc attgcagaga
     5941 gcttgctatc acttgcccca gtagtccaag gaagcattac aaacgatatt cccacctcaa
     6001 ttcctgagga acaaatgatc aatatcctga atgatgctga agttgttcgc acttctccgg
     6061 aatttacagt ctcgaaagaa tgtgaggaaa taatcaggaa caacaagctg gagacaaaaa
     6121 tcaattggat tgaaggttca caaagataca gatacatctt ggctactcct gacatttcgg
     6181 taaaggattt agtcgggcaa attgatgcta ttaaaattgc taagaaaggc gttgaactgt
     6241 atgatatcga atcatactcc tctggtcaat tgttgcaagc ccgacacgga attctgtgta
     6301 ttgacgaact tccggtcctc gatcctcgaa aacaagtcgc attactaagc gttctacagg
     6361 aaggtaaatt cacgactggg tcatatccag tggtctttaa accggatgta aagattatgg
     6421 ctacagcaaa tccagtcgac tacactcact cgggcaagat aatcgagcca ttgtttgata
     6481 gattacgtag tcacgtaaac acacactatc caagaaatac tcttgacgag atgctcatca
     6541 ttattcaaga ggcaagaatc gcagatgcag ggaatgtatt tctacctgtt tttatgcttc
     6601 ggacagtagc aaagataatg gagttagcca gaacccatca tggtattaat catgataaag
     6661 gcgtcagcgt tagggttgcg attcattcta cagaattgtt ggttggtgag gctgaacgaa
     6721 caagatcaat atctcaattt gtgaaggcga taccgcgatt ttgtgacatt catagtattc
     6781 accaatcggt taaatttgag ctaacagaga tagaagatac cctcgaaaca aggatcaatg
     6841 tattgaactc aataattgac aatgccataa gggaagtttc acttgaatat attcaaaaat
     6901 tatccgtcga acaattagca aaactgaaaa atgaattcac aaagaataag acattcttag
     6961 tgtcacaagt taccctaggt agtaacaaag ccaactcttt ggattatgaa tcgcagttag
     7021 aattgttccc tgagttaaaa caagttgtaa aggagacaat ttttgttata gagaatgagc
     7081 agaaaaaatt tgttgaaatc gcagaaaatc ttggaataag gagggagact gtatgcatta
     7141 gagaagacat gaacggagaa tttactgcag cggttactga agttatctta gaaggtctaa
     7201 ggtgggtgca accaccattg ttagatagaa aggacgacag ctatggtgcc attcaataag
     7261 cccagtaata ttaatcctaa cacaaaaaaa tttgtttatt ttgcggcgga gaaccttggc
     7321 aacaaacatc agacactgca tgaagagaag aatgcagcaa acgatctgct tgacaagatg
     7381 ttgaaggtga ttggcagaga ggtcctaagg gaaaaaactc cgactattca agaccttgaa
     7441 cgatcattgc aagatatatc agcgcagcaa caacatgaac aaagagacag tacaggaaat
     7501 aaggaggcca gaggacagga aacagatatc aagggaaaag gaataccgtt tgctgggact
     7561 cctattgttg cgcagttaat gcagaaagga tatctgaagg actccaataa atggctgact
     7621 agcaagggat tcataaacat cggcggtaaa atcttaagcg acgtcatgag agccctaaaa
     7681 actggtgatt taggggtgca cgaaacgact agtttaggta gaggaggaat agctttggat
     7741 tcaactagga gctatgagcg aggcgatgat attagacttg taaacgttcc aaagtcacta
     7801 ttaaatactg tgcatagact tgcgaagaac agttcttcgc ttaaaatccc attagatgtc
     7861 gaagaggatg atctagaaga gtatgagacg ctgcaagacg ttcgagcggc tgttgtttat
     7921 tgtatcgatt taagttcaac catgcgttat tcttcgatgt ttggtgacat gagtcggata
     7981 gaagcagcaa aaagagctct atggagtctt tttttactaa accaaaagtt tttcccgtcc
     8041 gattccatct ccatcgttgg ttttggcgct cttgcatcga aggtcgcccc taatgacata
     8101 ccctatctaa agacatttga acctggctct gactttttac attacacaaa ttatcagtca
     8161 gcatttagat tggcaagcaa aatacttcag aaagatggcg ctttaaacaa acgaatagtg
     8221 ctcataaccg acggccatcc gagcgcatgt ttcatagatg ataaaaaaga acaggataaa
     8281 attttatcac aaagaccata ttctcatttc tatataccag gtaaggaggc acttgatttc
     8341 aatcttgatt ttgcagcagg acagcttgtt tacctgtgtt atcggtacag acaagttgat
     8401 caatatatcg gagagaaaac gatcctcgaa gcaaaaaagt gtcacaggat gggaattgaa
     8461 atagatacgg tcatgatcag tgaagaagat tcgttgttga gatatgttaa cgaaatggaa
     8521 agatatgtta aaggcagatc ctattacata aatccaacaa caatcgataa agttcttctt
     8581 actgactacc ttaacaataa gaaactcacc attggatctc gatgttaatc ggatgagacc
     8641 cggtccactt gcaactattg gtttttatgt aaagagatta tcaagactgt acagatgacg
     8701 agtaagatga tggaagtcaa ttatctcgat tggaataact ctatcgatat attgactaag
     8761 gcccaccaag caggactctt tgcactgatt atagggccaa agggtactgg caaaacaacc
     8821 cttgtaagga agtttgcctc agatttaaaa aaggagttat actccgtaaa ttttagcctc
     8881 cgtactagag aatctcatct tattggttca aagactctgg aaaaagggca aattagtttt
     8941 gtggaaggga tactcgtgaa atctatgcga cacggtggtc tcttgtacct tgacgagtta
     9001 aatgcagctg aggctgacgt attattgcga ttagatgaag cacttgatga cagaagacag
     9061 ataatattaa aagaagcaga tgggcaaatt atcaatgctc accatgattg gtcggtaatt
     9121 gcaacaatta atcctctttc gcacgttgga actaaagagc tacctcctca gcttctcagc
     9181 agatttccga ttaggttgcg attagaatat cctcccgaag aaatagaact cgagatcgta
     9241 caacgccata cgacagtcga tatatctaag attaatgaca taaaacgtgc gatcaaactt
     9301 gcaaagaatt tgcgcgaagc agcggctgtt gaagaatt
//
LOCUS       DAOR01000214            4010 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_58407, whole genome shotgun sequence.
ACCESSION   DAOR01000214 DAOR01000000
VERSION     DAOR01000214.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_58407"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1877..1989
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1213 a          704 c          675 g         1305 t
ORIGIN      
        1 ttatgaaata ggaggatcga agcaatgtct tgctaaccac ggcgttaatg caacaatttt
       61 tggatatcct ctaaacttgg gatctgacaa accatcaata gtgaatgtag tggctaaata
      121 ttataatcta gcaggatccg gcagcgcacc attgatgttt ttaaactgta acggctactt
      181 gaaacaatcg cagaataatt gcggtacata ttcagccaac ggcttactta tgcaaatcga
      241 tatgatatcg agagtgatag cttcagacat gtagatagta atcacaatta tagccctcct
      301 gaggagtttc agaaattcgt tcaacgtatg aatagtcaaa ttccatacga tacaaacgga
      361 aagataaatg caattcctat aattatatat cacaatctta caaacagcat gcaagattat
      421 aacaagatgg cttcaaccat aactgtggat ttattcgctc aacagaggga tatgtataat
      481 atgatccaaa caataatgtt tttgatataa agaatgctcc attgtctgct tatggaactg
      541 caaccaacac tgctaccgtg acttattcct aaatattcgg ctaatttaac actaatcttg
      601 atagcttcat ccgatatagt ttatatcttg tgatgtgtgc atgggcgtaa atagccgact
      661 cttttaataa ccattagtgc tttcctagat ctgctcttcc agctccaact atttgattat
      721 tagagaatac attatccttt gatgtcgaat cttgaacgat ttttaaacct gctggtgtag
      781 aactaactat tttattagca gagaacgtat ttcctgatga accattgttg atgagtatag
      841 cctgtgagat agagttggct attgtatttc caaagatatt gttatcagaa gaaccaggtc
      901 ttacgtcaat tccatcgcta ctctttgata tcatgttatt agagatctga ttgttatgtg
      961 attgtgaaac agagattcct gtcggttcgt tatatatgat attgtttctt acaatcgaat
     1021 tatacatatt tctactgaaa actattccat ctccagcatt accatgaacc ttattatttt
     1081 caattagtat attgtagcaa ttcagagagc aaattatacc acttccatta ttatcgtaga
     1141 ctgtattatt tctaattatc atatcatgtg ttcctgtatg aggatcaaga ccataatggc
     1201 cactattacg gataatatta ttttcaacaa tcatatgacc aacaccaaaa gtgtataaac
     1261 cgaagtaaac ttcacgtatt atgttattcc ttattacact tccgtcaccg ccataataat
     1321 aactcagacc tgaacctcct ttgtgcttac cttgttcata gcctagatat gcaatctctg
     1381 aattggtgat atctgtagtc cctgtggcat tattttcaac tacgatggat ggcctaggag
     1441 caccaaaaat aaatatacca cttccagttc ttgacccgtt tgttatagca tacttatttg
     1501 tggttggatc ccatgaagtt atctttacca aatcaatctt tagactgcca aatacatcta
     1561 taatatatac taatccaatc ttaggtccgc cgctgcggtc tacttttgaa ttcattttta
     1621 gccatttagt gtctgtagaa tcaatgtgaa aggtagctcc tttagatatg accaactttg
     1681 cgctaagaaa ccatgtgcca gctggagatt gcttagccag aatactacta tcatggagtt
     1741 tgttatcgat atctgtaagt ctagctgaat tacagctgac agtaatcgtt cttgtagacg
     1801 gatcgtaatt gatgcaacca ccaggagaag cggtgttgga ttgagaagag ggagaagatg
     1861 caattgcatt gccttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnna taataagatt catgataaca cttatctaaa ttccacaaaa gccatagata
     2041 cgagtgcagc cggtagatct ggtaacttga tctataataa tcaccttatt aactcaccat
     2101 aatcattttg aaatctattt ctaaattttg agagttccat ctttagcacc aagcaggaat
     2161 agcttgtagt cgttagttca tgtcagaaaa cgaaggtgtg catgctacaa attcagtaaa
     2221 agccttgtgt ttgatagctt ttgcctcttg aatggacaat ggaagaccgt tgaaggcgct
     2281 atactgagat atgaggagca caagagccaa ggtaaatgca acgaaaacaa aaaatggtac
     2341 accagcccgt gttaagcaca atctctccct atactgatgc cttatcattg ttgatataat
     2401 catcactttg ttattccaaa tgagtcattc attttttgta tgtatgggcg gtatgttcaa
     2461 cagacctctt ggttttatcg caaaattttg gcttagggtt attgggcgat aatctaatta
     2521 tttgattatt attattaaat agcttatttt tcggcattat cactgagatt tcggtactcg
     2581 cactctggcc tatcagagaa taacgttagt cgctattcct tttagcactt acttacttaa
     2641 agaactccct taaagggagt aagacttatc tttatcacaa atcaataatg ttgattgtta
     2701 gaaggatgtt actaggtaat aaaaaaagtg cttgtggtag aactaagggt aggtagaatg
     2761 gtgtttttat ataaatgaca gacccgttta ccctattgta tccatcatat gccaccacca
     2821 ccactactac tactattgct attcatcacg gtacttatgg tgttgttagc gctctaccaa
     2881 catcattatc attatttagt ctagtagtga ctgttgcctc catctttttg tgttctgtaa
     2941 tagctatgta tttttacaga tcataccgat tttctggatt cggatatcta ttaggcttac
     3001 caactggttt tgcaattcta gctgcatctt ttgtttttga gcacctaaat gtaatctatt
     3061 cttattcgta tgctaatgaa tccttagaaa atctgttctt ttggatacag ttagctcttc
     3121 aatcagaagg atttgcattc atagctctct catatatgtt gaaaaaccgt gctggttcta
     3181 ttagtagtac tgctaacaac aggttattac cagaaactta caaagtattt ttctctccac
     3241 ataattggat ctattcccct attaagatta gggaaataat agttggcgtt ttacctatga
     3301 ttttaatttc tattccatta atggttacaa tttctgcatt atttgttcaa ccaatactca
     3361 acgagactga gcttcaagac atgagtcttt acacaacgct atttgacatt gtggttttag
     3421 gatatatctt cgtcaaatgc ctaaagcccc ttgtaaaggc agctaatact aaattattat
     3481 acattccagc cgctttcgca ttgctctggc ttgagcagta ttcgttaata attaactatt
     3541 ttgataataa ccagtttgca tttattggat caataattgt tagactagct ggtttgagct
     3601 tgttcgtcta tgctatatat tatgccctat ctagtggaag gagtagagaa atggagattg
     3661 agacctgata catgagacaa gagaagagac acaagctcca actcttttat gaaatactta
     3721 tcgctattga aggatatatg agtaataatg acgttacagc tgcaaaacca actcatattc
     3781 agcatactag tagactgtca tatgataaaa tgatgaacca ctttaatgag ttagaaaaga
     3841 aaggtatgat aatttatcgt cgaagtaata ctgacgtatt gatatcgata actaataagg
     3901 gcaaacaatt cataaagcag tataacgaac ttatgattct aattgaaagt gctggattat
     3961 aatgataact agtgtcattt atagggtcta gaaaataatc agccaatatt
//
LOCUS       DAOR01000215            3338 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_7925, whole genome shotgun sequence.
ACCESSION   DAOR01000215 DAOR01000000
VERSION     DAOR01000215.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_7925"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    143..345
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          999 a          590 c          584 g          962 t
ORIGIN      
        1 atatgtaaca aaattatgat atattactat tggaatggcc cttattactc cgccctgatt
       61 gaacttaagt tggctgttta catctgcgat gaatacctgc aacatctgcg agttatcata
      121 ggaatgacaa acacctgcac acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggaat gacaaacacc
      361 tgcacacact gttccttctt gggaagaaga gtacggacca tcaatatgtc tatgagtcca
      421 actcttaata gaatatctat ttgcgaaagt taattttcca tcattagaat aagttctaca
      481 atcaatctgt ggatgagttt tccagccatc gcaatgcaag aaagtgactg gaaagccagt
      541 atccgtcctg gctaaggaat agtatctagc aacagtacta actaccgcag gattattcca
      601 tccttcacta taggggtatg caaatattgt tgggttaatt ccatgttcaa gaaagcattg
      661 ctttgcacca ccaatttcag agttcaattc aatctgagat aaggtattta gatgtggatg
      721 cgtcatagta tgtgactcta tattcatacc atcctgttgt aatgctacga tatcttgcca
      781 agatttccta tcacttgatt ttcctatcca cccacaaacc tcaaagaagg ttgccttaaa
      841 tccatatttg tctagtattg gcttggcata caagaactga ttcttaaatg agtcatcaaa
      901 gtttatgatt actatcttat tgttgctacc attactgctg atgccattag tagtagagga
      961 gataccagca ggtaataatg ataaagttgt aatagaacta agatgggaac ctttagaaga
     1021 cctaacagtg ccccgatgtt cagttccttt tactcctgtt gttttaggtt tgtctttggt
     1081 aaggcttact acttgactta ttggaaattg ttgttgttga tggctgggca caacaaatgc
     1141 aaactgacta taagctgcaa ttggcgttga aaatatgaga cttaccaata caataataat
     1201 aggaatattg gatataattt tagcagatca gaaggtaata actgcatgaa gatatcctaa
     1261 tgtggtgata taatatataa aagatgatta tctatgttaa tgaagaacta attcactgaa
     1321 ataaaatcta atgcaaagaa catcttccaa ctacatggct atatggcacc tgatacgagt
     1381 tcactttgtt tcttagagcc gcataatcag tgaagattta atatacttta acggactatt
     1441 atttttatag tgtcccataa ggcacacagt aattcttccc ccgatgattg ggcttcacgt
     1501 gagaagcata aattgttaag agaaatagct atactatcat caatttttgt tggtttgata
     1561 atcttggcta ttagaccatt tggatttaga acatttctaa atggactagg gcatgacaat
     1621 atattctctt tattgcttgt agcttccgct gttgtagttg tattggttat agtgcaaaga
     1681 ataggcttga tatctccaga tgaagaaaaa agggaagatc atacctagta taaaaaatgt
     1741 attcttagat acacagtctg cccgaacttg taatggtttc gtcaatctca atagcttttt
     1801 atgacaagac aattcactag atagatctgt tgagaaaaga aacggctata gcagaagaca
     1861 atgcaatgat aagagtagta acaggattag tgccacaaag ttaaagatga tgtctaggat
     1921 gaacctttta ctttattagc agcattggga gttaaagttg cacctccaga tgaagttgta
     1981 gaagtaggcg atggaacatt ttttatataa aacaagttat tggtcgtatc atatccaagc
     2041 tgattaattg tcagtacctt aaacccataa ttggtacaaa aaccaatagc cagcgttgaa
     2101 gtaatctaac gttaaacatt ctctttatgc aatatacata gcaattatag aataaaaaca
     2161 gacacacact tttatccaaa ttatcgccag aatctgtatt cattgcccca aatagtacac
     2221 agcgagcaca actcttgcag agcgcgtctt ggagctcaca cttcataagt gcatctcttc
     2281 atctcgcatt tcgcgaacaa ccttagagta atatccttta gtaaaggaag caaaaaaaac
     2341 gagactctgg cgacttttgc ttacaagcaa ggaaaactac tctagccgtt tgagaagaat
     2401 cgttaataga tctgggaatt accactgtgt taatttctct tgtttcttca gttctgaaaa
     2461 tacgcacgca actcgttaat ttttttggat cattttccct acgtttcgta tggtttgagc
     2521 cttccggagc tgtgtggtct acccgtagtc ctctttacct tgacttcaca ggacattcgt
     2581 cgaacatact atatactttt tataatattc atttgttgga tctgatcaga cggtatggca
     2641 agtaatagta gtgatggaca tcctactcgc gaccaaaccg tcacggacgc aaaaatacag
     2701 ttgttgacaa atcaaaccga gaaggattac gctttacgct tgaaaggtaa atctgaatta
     2761 cttgcagcga ctaatccata tgccgagtat acaaaagtta ttgcagagtc ggactatttc
     2821 gaagattttg agtataggaa ctggaattat accctgaagc actggatcgc ctataaaaac
     2881 agcgatagaa gaactactaa caacaaaaaa taaaagtgat aactctaaac ccacgcagcc
     2941 tctcttctgc caaagatcac aattagcctt ccgagtgaga tgaaagtatt gccataccga
     3001 tacatctttc tttccaaact tgaagcgcag gtatattcaa ctgtgggaat gccgcaatat
     3061 gacagatgct tttctgctag agtgaaagtt gaaggtcaca tgtttgctct agatatttgg
     3121 cttgatacta taaagttcct ctgagaaagg cctattgcct tttgaaaggg cgcacgatcg
     3181 aatagatcct agttttcgat gtttgaagtc gccactacta ttggctttca gttgcttcca
     3241 cacactcgaa caggctaagc gtaaggtgta actgaccgtt gacactacga tattctaatc
     3301 cttatctcgt cgtagtaaac ctatagtcga atctattg
//
LOCUS       DAOR01000216            2967 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_58057, whole genome shotgun sequence.
ACCESSION   DAOR01000216 DAOR01000000
VERSION     DAOR01000216.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_58057"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    388..662
                     /estimated_length=275
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          876 a          514 c          508 g          794 t
ORIGIN      
        1 acccccttct atagaaatgt ggtagtaaga gatgtactcc aggcactgtt ataccatcaa
       61 gatatgattg agatttttca gttgaaagaa cattctcatt ttctattttt ttaagatgaa
      121 cctcttcatt agctttattg ttcataataa aaacataaat ctctaatacc agaatagaag
      181 acatacgaca acctgaacta tatagaaaac ctacatatat ttttctgttc tacctggatc
      241 aatgcataaa caaacaacgt tcgtaataac ggcagcaatc ctgcttaccg caatcgtgtc
      301 agtagcactc tatactgtca aagcagcaaa tgcacagggc aatatgacta gtcccggtag
      361 cgctaacatg acaaagaaca tgacaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nngtgccgct ggtaacatga caaagacaaa gatcatgata gtaaagtaac gcgagtttat
      721 cctgatcggc gtgttattag cgtatactta tttttttggt atctctgtcg aacaaaaaat
      781 aataatcatg agattgtttt ttcaaaacat caagctcaat gacggtagct atttagaaag
      841 tgctatgaat aatactgatg gctaagttga tgatcctgat ttagtattaa acagttttaa
      901 gactggcttg tatgacttgc tgctcttgga catcaaaatg ccggaaattc gatggctttc
      961 gattgtacga ggaactgaag aagatggata ataaagtcaa ggtctgtttt cttacagcaa
     1021 gtgattccta ttatgagaaa attagacaag aagaatttcc tacactagac aaagatttgg
     1081 ttctttcaaa gccccataga aaatggtaat cgtagataaa cgaacaagac aagaacacgt
     1141 acaagaaata gctccaacta taagaggaat attttatgta ctcgtatcgc ttagcgttat
     1201 agctaacacg attcaagaga ggaggtatta atttccagag aaggcaatga gctctctctt
     1261 atatgcctga cgttttcttc cacaaagcag agtgaattac gtcacaatca gtggcattag
     1321 tgagagaact gggatcacaa agaggaagtt ttattattat ttattacgat acgtatagaa
     1381 aaagactcaa agcttcgtcg tatcagcggt acgaaattaa aacaaacctt ccaaatttac
     1441 acgccatatt tgattataat gtgtttttca actcaacacg tcagtataaa atcacacggc
     1501 gctctagacg atgcgacgaa agaaatctta gcctgcctac acggatagta ctgatgaact
     1561 gatgatggaa caacaagaga tgggcaatgg aatgagcttg tagtcattcg gtttaataga
     1621 tcagctttca aaatatattt acggattgac aaaactaatg aaacaataaa taccaagata
     1681 atacgacaac tacagcaacg acatgaataa gtaaatgaca atggtacaga agttgaagtt
     1741 acactgccga tagtagatga agtgaaaaat gatgggtatg aggtgttttg aagattactg
     1801 aaaatatact ctctttagta ctgacatctc gtttaaattt tatttaacaa acgatgttga
     1861 tatgttccat cgtcggtaca tccagaacat aataagatat tatttcatta tccttctttc
     1921 tccaacaacg aaataatcgg attttcatgt cctccaaggt attcatccac ttgcgtgatt
     1981 aactggagtc ttttcccgat tgaaaatttg atggtctttc cataactggc tttgctggtt
     2041 tcctataaga tctgtttctg catttggcac atgaatcagg caaagtcatg taattcgtcc
     2101 gtttctttcc acgcttatac gcaaaggagt taggccacca ttgatggcca cacctcaaac
     2161 actgaatcat catatgttct ctgtcaacat aaggtagcgg caccacaggc gatctttctt
     2221 gtacagggag atcccaccct gaacgtgcta gttcctcgtg agcctcacat actttgaaat
     2281 ctcttataaa aattttctgt ccttccaaaa tgcctctctg tagttcggtg aacaaaaccg
     2341 ccagcgattg gcagcttatc acgtcacatt tcacttcctt gtacgccgga attctaaccc
     2401 ttcgtgaagc cataaccaga cccattgacg aatctgggta gtgataccta aatgtttgat
     2461 ccgtttcaga tcgacgcaaa atattgattg catagtaggc atccaatcca aaatgtcttg
     2521 aattttaaag attaaatacg atttatctga aacaatgtag aatatttaag ataaacgtta
     2581 gtttgtataa taaatgattc gatcggttga ttttcaatta tttcctgttt cttatccaga
     2641 cgggagattc gatatgcaca cgcgtgcaaa taactgtatc aatgtaacga aaacgactcg
     2701 tctatggcct tcctcccggg aggaaggcca caaacatcgc gtgtatcggc gtacaggatg
     2761 acaaagactg ttagtttctc ccctgacgag cgggtacgaa agtactgccg aatctaacag
     2821 tcgattgagc ggaaattgag ctattgagag gaactgtgct ttgaatttga actttcactc
     2881 ggttttttat gcaactgcca atttcggatg cctatatctt acattaatga tgatttctga
     2941 atagaaaagt tcctgcgagg aggaacc
//
LOCUS       DAOR01000217            7489 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_77146, whole genome shotgun sequence.
ACCESSION   DAOR01000217 DAOR01000000
VERSION     DAOR01000217.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_77146"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1989 a         1679 c         1447 g         2374 t
ORIGIN      
        1 tgcaggtgat aatgaatggt caatagggtc tccgatgata caataggttt taaatgagtc
       61 ttgcattgat tttgcctgag cggagatcaa tcctatcata tagctttctc atttgttgta
      121 ccgtcagttg acctggggca actgcttctt ctagggcagc ataggtaaaa ggcgcatttc
      181 catagatcgt acatagaata cgagataaaa ttcctagttc tcccattgcg aatgatatta
      241 agtttagttc ggttgtttcg tcatacagat cgagaagtcg tatgcagtcg tgaacatttt
      301 gggcagcggt agcgatcttc acgtaattgc tgtatatcct catttgccct aaaacatcta
      361 cgagtttgtc attagtcggc gttttttcaa agtcatgcca agaaactaat atccgagttt
      421 cttgttcagc aatgaaatct actaactcgt cgttgtcaag caaagtctcg agttcaacat
      481 cgagcagcat tggcttggat agtgacagtt gctttaacca aaaaattcga tcttcatcgg
      541 tccctgtaaa tttgcctttc tgttgcgccg acctgagagt gaaaaccgcc ctctttctta
      601 tgcctttcac aatctctaaa gattcttgca tatctgccag catcaaaaaa tcgaatcgaa
      661 tttcgacgaa gtctgccccg agggtaaagg ctcgtttgat ttgcttcttt aggtcttcag
      721 gcgttggcga cgctatcgat gcacaaatct tagtcaatga taaatcatct tctacctaat
      781 gatcgctaac cgactcccgc tggggggtga cttgatttgt cgtgttccat ccgttcatgt
      841 gcactcaaag cctccatgct gtcgaacact cgtccgcacc gactgcagcg gtgtgatttt
      901 ggagacttaa tgttcgatga ggtagatgcc gccatttcct atcaggttat tataccacca
      961 acagataatt aaagatgata acgctatagc ttatttgagc aatgagacgg aatttttttc
     1021 cagtttgcaa tcagcgcttc agatcttata tgcagttgtt cctccgcaag tctcgcgatt
     1081 gctagttgta tgtattcgtt cactatttct attcctatgt accgtcgatt gaagtgtgat
     1141 gccacctttg tggtctggcc gctgccattg aagggatcga gaaccgtgtc tccctcgtaa
     1201 gagtataact tcataagtct gtacggtatt tcttccggaa atggacatgg atgcgttatg
     1261 tatcctggag gtacaggtgc aatatgccaa acggaattgg caatctcttt tgtccactcc
     1321 tcgtgcgttg ccggaagtgt ctcgtcgcgt tgcgttctgc cagactccac gtttcctttc
     1381 cttaatacta gaatgaattc gtgcataata ttagctctat aatactttgg atatggattg
     1441 attacaaaag agccatatct gttagtacca ccggtcacct tatgccaaat aatctcctca
     1501 tgaagattcc attgaccgga gggttgaacc agtctggata ataggatatg cggtaacggc
     1561 agaatcgtcc cattgactac ttcgttcgcg ataataatac aacagtaccc cccctctttg
     1621 gttactttat agaccttatc attaaaaatc tctaccattt catcaagata atcgcttgtt
     1681 tcaatattag acctgccacg ataatatccc ccgtttccag aggcatgcat ttcgtaatca
     1741 attgcatttc tatatggggg tgaggttatg gtcaattgta ccgaatgtgg ggaaattctc
     1801 ttcaatacct ctttacagtt gccaccaatt attacattaa caatattttg ctgtacctgc
     1861 atcaatgata tattattgat ttgtccatat tccgaatgct ttaaaataat cgactttccg
     1921 attaaaaaaa tatttcctgc ctatagttac tattgtactc atttctcaat gatatactcg
     1981 tcaacttcta ttccaaaatg acgtccagtc ttagggttta tgaagccgag tatctcatcg
     2041 ccgactttta cctctgtgac aggtatcagt ttggcatcgc tagtcataaa agttattgtt
     2101 tccgcattct gcaatattat agttccgacc tctccattga tctctcctct aaccaatttc
     2161 agaggccgag tttctatttt tgatcttccc accgttacgc gtctagtctt cccctcttta
     2221 ttcactatga ggacctcagt gccagattcg atctcggaga gatatttggt cttaccacct
     2281 gggattattg tgtagcagta aatcgcgcct gcgtttatcc tgaatggcct gggcgatgta
     2341 aatgcagatc cttctgattc attgtgtact aagaacataa agttagatct gcttccaatg
     2401 agcatgcctt cgcctgtggt aagcagggat gcagtatcta cacagactct ttcaccgcta
     2461 ccaacctctc tcacctctaa aactttgaca ggtctaataa taaaaactgg attatcaagg
     2521 tactgtcgcg atacttcaac ttccttcgga ttgtcggtcg aaagtataac accatcaacc
     2581 cccaattcca aaatttcaaa catcgttctt acttcactcg gatttttagc gagagtataa
     2641 accttggttc ccgatttatg tagtttggag attatatttt ccaagggaat gatcttccag
     2701 ttaggagcgt caactatgac aaactctgcg cctagtgtgg acatcaatat cacttcttca
     2761 agatcttcat tactgaagac ttttttggaa aagccaggaa ccttatcagt cgactttgca
     2821 ttccttaatt catctattgt tttacaaata atcatgtctg catcattcga ttcaaaaatg
     2881 gttttgaatc cgctcccggg agccaacttc ggatcgatat tgagaattct aacatccttt
     2941 aactccgaca agaaagcatc taacttagat ttagcaacag ttggactaac aattatctct
     3001 ttagtctttg tgtctgtcaa gatgtaagat cgcctcccta gtacccttac cctctatgat
     3061 gattttatat aacgcacgga ccattgccgg cggattacgg tgttggaata tatttctacc
     3121 aaacgtgact ccgctcgctc ctgcctccat tgccccagca cacatttcaa taatttcttg
     3181 atccgtcttg gcctttggtc cccccgctat cacaataggg actgggcaac tttttatgac
     3241 ctttctaaaa gagctgacat cgcctgtata aacagttttg actaaatccg ctccggcttc
     3301 agccccaatt cttgctgcat gagcaacaat ttgcggatca tgaggatgtt tgatattttc
     3361 gcctcgcgga tacatcattg ctacaagtgg cataccccat tcatcgcatt tatctgagat
     3421 gattcctaag tcgtgcaaca tttcagattc ttcttttgcg ccgatgttta tatgaaccga
     3481 tactgcgtcg gcacccaatc ttattgcttc ttcaacactt cctatcaaaa cttttttgtt
     3541 tgggaatgat cctagtactg tactgcccga caaatgagct attaagccaa tatttatggg
     3601 agttggcagt gttttgatga tgcctttgtt taccagaacg catgtaagtc ccgcgttctc
     3661 acactgataa atcattccat gaatattctc caaaccattg atagggccgt tgctgatccc
     3721 atgatccatt ggaatacaaa gcatcttgcc attcttcaag agtctcccca ttcggatctc
     3781 tttgccgcaa accatatata gtgaaaccat ttcctgacct acttaaaagt gatcgtctag
     3841 ggaacagcgc cctctagagt atcgtgcgct ttttaatatt ttgggctcgg cactaaaatt
     3901 tgctatagtt tgaggctggt aaggttaaat aatttggatg gtacttatcc tttcaattgc
     3961 aaacagctct ccggaaagta ggttcttaca tgattcgtcg ctgcgaggca aaagacctcc
     4021 cgacagtggt agacatcaat atgcttaccc ttcctgaaca ctattcggat tacttttttg
     4081 aatcaatcct gagggaatta cctgaatcct tcatcgtcgc tgaactttgt gacaaagtag
     4141 ttggatatat catgtgtaag attgaattcg gtttctcgaa ttttagaaag ttgggtttcg
     4201 taaaaaaggg tcacgtggtt tctgtcgccg ttttaaatga acacagaaac ctaggccttg
     4261 gcaaagctct gatgcttgaa ggtatcaacg gtgttattgc tcggaagacc gatgagattt
     4321 acctcgaagt tcgagttagc aatgaatcag ccgttcggat gtatgaaaga ttaggatttc
     4381 aaataaaatc acgacttcgc gcatattata gggatggaga agacgcatgc cttatggctc
     4441 tagaattcgg gtgaatgata gagcgatcgc aattccgaga cgtctcgaag gtatgtaaat
     4501 aagatggtaa gagcatccaa atagagatga aaagcagaag agtacgagga agaatcctgg
     4561 ggactggtct tctagctgca tcgattatgg tatttattgc gtattcttat gtaacacttg
     4621 cgacatcttg gggattgtta gtgttgaaac ttaccatcgt tgtggcagta ggcgcattgt
     4681 tagcagtctt ggcatggata ggctacacga tgacaacggc tcccgagcaa gccgaaagtg
     4741 acaagtctaa ttagacttgt cactacagac acacgtctga cactatttga tacaatcttg
     4801 gacctacttc tctcactatt cttgtttgac gaatgtagta ttgataattc ttgaacgcat
     4861 tttctgtatc tattacccct tcgtgcaggc cgagtttcct attgcttgcc cctacatgag
     4921 caaagtaatg tattgtcccc ttcccttcgt tcaatgcctt tttcgcagag gagacaaatt
     4981 ccttagcctt ttctggcaaa ggcatcaata ctctattaga tttaccatca atttgttttt
     5041 ctataactcc tcttgcatca ccaaggattg atataacatt gtcttcaacc ttgtttaatt
     5101 tcgcattaat tctacacagc tcagtagcaa cagggtttga atcaatactg tagactgtac
     5161 acgtattgtt ctttttagcc atcagaatag aatatgtccc tactccgcca aacatattag
     5221 taattacctc attgtcacgc actagctctg ccaccctcaa cctctccgtc gataatcttg
     5281 gagaaaaata ggccttaacg acatctaccc tgaatcgaca tccatgttct ttgtattctg
     5341 tttcagtctt gtcttccccg gcaagaaatt ctaaattacg tactctaaat tctcctttga
     5401 ttgcggatgt ttgaatgaag acggactttg ctggtttaac attgtcaaga attgcctctc
     5461 caattatttc ctttttcgac attaaacact ttggaatctt tataatgact atgtctccaa
     5521 ttatgtcaaa tgctgaatag atcctgtgct gttctgatgg ggtgaggtaa gtggctaaca
     5581 cctctttcag catatgtggc ataactaacc agaagatttt tttgtgtaat aataatctcc
     5641 agaagttttt tgcgacctat ctaatcgact accttccttg ttgattctgg gtctgcctgt
     5701 attctcatct cttctatatg taatagagag ttcccttaga aatttattca tacctatctc
     5761 tttctgcatt ggttggctcg ccaaaccctc tagacccggt ttgccatcga aaattactaa
     5821 agaatcggct acaaggtcta tgagttgcat gtcatgatcg attacaatag cagatttacc
     5881 ataggccctt acaaatcgtt gcaagaattt tgcaaatgcg attctatctt ccacatctaa
     5941 gaatgcggac ggttcatcta gggcatagat attggcagga cgcattaaag ttgcagctat
     6001 ggccaccttt tgtaattctc caccactaag attcctgaca tttctttcat agagtttctt
     6061 gactccggtt ggaattataa tctgttcttc catcgaagaa atgtcggata cgccttcaca
     6121 tgctgcagaa agcaaagtat agacattgcc atcgtaatct tgagaaagat attgaggttt
     6181 gtatgaaatt tttgcactta actctataat acccgagtca ggtttgtcaa gcccggcaag
     6241 catcctcatg aaggtggtct tgcccagcga attggcacca atcacacctg cgatttctcc
     6301 ttgtcgtatc cggccagcca atattttgag atgaaatgat gggaatgatt tggttaattc
     6361 ggaatagctc gcgacggcag tatccagaat aatgtcctca actgagtttg tagtgtcaaa
     6421 tcggaaagct ttttcacgaa agcgtacgtt ctcggtaggg ataaatcctt ctaggaagtt
     6481 attgatgcca agctttgtag cctgtagacc agaaactatg ccatatgcgg ccggctctcc
     6541 atatacaata tgtatgtagt ctgaaagata gtccaacaac gtcatatcat gttcgacgac
     6601 cataacgctc ttgcccaggt cagccaagcc ccgtatcact cttgcaacta caagtctctg
     6661 gtaaacatcg ttaaatgatg atggctcgtc aaaaaaataa tagtcggcat ctttagctga
     6721 agcaatcgcg actgctaatc tttgtaattc gccaccactc aaatttccaa cgttgcggtc
     6781 aagcaaatct cgtaacccaa gttcgtctac caagtcatcg gccgcatttc gttcgtcata
     6841 tttttttagc agttccttag cggttccctt gaaagcactc ggaataagat aaactaactg
     6901 aggtttaatc gaggctcgta tctcgccgcc cgcgattttc tcaaagtgag attgcaattc
     6961 agtgccctga aaatttttaa gaacatcgtc ccattttggc tgggagtcgt aattgccaaa
     7021 attaggtgtg attgatccgg ccagtatatt gactatagtg gatttgccta tagcgtttct
     7081 cccgagtaaa cctacaacat gccctttctt aagagttgga agacggtata atctaaatga
     7141 attaatgcca tactgatgaa ttttttccac cccaagttct ctagccaggt taacaataac
     7201 aatagcatca aaaggacatt tctttacgca tataccacag ccggtacaaa gatcttccga
     7261 aattactgcc tttttactat catcgctaag tactatacat tcacccccag tcttgttcac
     7321 cgggcaatac acgatgcatt cgagaccaca ctttctgggc tgacacagtt cctcgtctag
     7381 aacggcgacc ctatgtgtta gctttgtgga atccttcatg gtccgaattt gcatcaatgt
     7441 attatatagc ttcgtggttg cagtgggtca acttgtgttg caaatagtc
//
LOCUS       DAOR01000218           14010 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_38237, whole genome shotgun sequence.
ACCESSION   DAOR01000218 DAOR01000000
VERSION     DAOR01000218.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14010)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_38237"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5002..5256
                     /estimated_length=255
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4435 a         2598 c         2594 g         4128 t
ORIGIN      
        1 gagtgttggt agtaagaaag gtgctaaaac aacaaccaca actgattagt tcagcttggt
       61 aatttaagat tatatcacac gtcttgtcct gatgcttgat acaacataac gagattagtg
      121 atttagaacc gaagaaagga attagacgaa acgagatttg ctttgaggga tgtaatgctt
      181 agaatttacg acaaactgga atgtagagca gcgaaaggaa tgtgtgtttt ttttcttcga
      241 gactagcaac aggtgaaggt aaacaaacat ctaatttttt tctaaaggag aaagagagat
      301 gtgcctgact ccaaaggaga aataagccac atttattcct ggtagaggtt cctatagtta
      361 aagcaatctt ttatagctac tatttgaata gttcgttcgt ttgtgtttgt tggtgcctat
      421 tgatcctatc cttattgttt ctgctgctcc ttcaatagtc ttggggtcac actcacgacc
      481 acgagtcctc tgaagatgct ccactttcga cggattcttg ctaaagtttc gcaaacattg
      541 catatgacat caacctctag tgcaattcta ctgttgattc ccaattccat taattccatc
      601 ttgtactctt cgaaccatcc tctaaccttg tcgacgtcgg ctgagaatat ttgaaaaaaa
      661 ggtagatcat ataagtaaac agcgaagaaa agttttatcc aagtttcgca aacttttcag
      721 ccttaaaaag tagtgttact tttgcccttg tatgcaggtt agagaggttg agaataatga
      781 accacactat accatttact atcaataaaa gatatctgtg ctcttgaaag tattattctc
      841 cttaacagat aaagcggaat aaggtaatta tgttgtaact ataatctttc taatgaatat
      901 attagtcttt caatatacca acaaattctc ctagattttt agcgcttgct gtgaagcacc
      961 tcaaatatag catacataga tagttttgct gaagaggata cggaggcaag atttgatctt
     1021 ttccttaaat tctagggcag ttgtgtcgat gttcttggtt gttgttttgt tagattctct
     1081 tgtttttata tatggttttc aaatggcgat tgcacaacaa agaataccgc aatcactatc
     1141 accaccagaa ttatcaggag ccaatgctat tgctacttct tctaattctc ctcatgcggc
     1201 tgccaagata gttcatgtta acaagactgc cgggcagtca gaattgacct cgatcatagt
     1261 cgaagtaagt gttcattacc ctacaccgca agaaaaaggc agagtagata aaatcgttaa
     1321 tcagacactg agtatgttac aaccgatagg gagggcgata aacgagtcgc atagttgcat
     1381 cttagaggcg gttatatgcg gtaatagtaa tatgaccaac ggaacaatgt tgagattata
     1441 gtccccgcat tcacaattga ttagacaaac aacaacctat tcatactagt tagctaaatg
     1501 ggttgtactc ttatagacat ctttcatttg tctgtgcatt attttccgtg gcttgttctg
     1561 tcagccaatc aatattttat attcttaaag aaggatgcac cccactaatg gccacattaa
     1621 gaacgcacac attgggtagg ataggtgatt tgtacacaga tatcgcaaca gaaggatgat
     1681 attgtatggc gtccagttga agggaattaa atgattccaa atctttcttg atagaactag
     1741 ttgctagatt aacccaaaca ataactatca agattattat tcctacaaca atgtaaataa
     1801 gataccagag tcattgatat cattaatatt tcattaatgt ctgctgttgt tatttccact
     1861 cagttattac atttaacaca tgaccggtag catccctgtg ttctatcgcc gtgtgtttgc
     1921 ctgggaaatc cacattgcat ggattgcaat gatatgtttc gttcatatat gatgttaatg
     1981 cactctaaag aatgaggtag ctaacgatta tgacctcgat tgtttctcgt cgtctcctgc
     2041 tatcttatta ttagagacaa ctgtatttac agaaatgggt ccggttaaat tattcaggaa
     2101 tacaaataga ccatatggac ttcttgcagt agaagatgag ggcgaagtca tttgggccga
     2161 aacagttagc gatgaagcaa agatagctgg aaatagggtt ccaaaggaaa aattcaataa
     2221 aaagctcata caagaggaat gggaacatga cctcaatact aaattcacct ttgctgatgg
     2281 agaatttcaa ttgataaaac agaagaacac cttatgataa tcctgggtat tctcctatag
     2341 taacaataat gtgtgcaatg tcgtgaaacg aaccttactt tgtgtcacga aagagacggc
     2401 gttggcattg taacattgac ttgtcttgga aattgaatgt catcgctata aacagaggac
     2461 ttttatgata tatatctgca atccaatgac gggagaaaga gcacagaatg ccgcggaatc
     2521 acatactaca taggatctgc agtagtgaaa aaataaagga taacaccttt cagctcttta
     2581 ccgttgatga tactgacatt gtagtaggaa gaagatacgg aaaattattt gccttcaaca
     2641 attcctgtcc acacagagga gcctcactct caaaagggga attaaaggaa gataatatag
     2701 tatgttatat gcatggttac gagttcaatg tttttacagg aaaattggag aatatgaaat
     2761 catggaaaaa acaagataca tggttagagc agaatccaga atggagaaag gcaggcgatt
     2821 tagtcttgta tcctgtgagt gagaagaatg gaagtatatt tgtagatatt tgaatgacaa
     2881 ttcctagcca caagttagct cacatttagg ggctgacttt gatagacttt atctttgtat
     2941 ttttccgctt tatttcaatc aatggccatt acaccatctc ctaaaaagct gcttctgcta
     3001 ttaggggctg ataacgtccg tcattcacta cacatgcaac tggacaagtt cagcttgaaa
     3061 aagattttct ttgcagtata tcctaataca gatacgaatc agcttgttag ctgtgattag
     3121 tgtgccggtt ttgtattctt taacactatc agtgtttaat tcaagtgcat atgctgtagt
     3181 tatcatattc cattcatccc gtaacttttg gaaaaaaaga ttcttccaga caagaatatt
     3241 tctgcttcgt ctagaagcag ttaaaaatag agaaatgaag tgctccggca actgcataac
     3301 atctttgtta tcctcagtcc atttttagcc acgtcttcaa agctgtcttt taaagaagtc
     3361 tgtaattctc atttgttctt tagtattctt atagatttta ctatttctaa tccattcatt
     3421 tttagatatg gatagattaa tacatgcgaa cgacaatgca ttctggaaat tgtcaaactg
     3481 ttttgcttct ccatcaagtg ctttccttat tccctctctt atctgccata ctcctaaagg
     3541 cataacatat tctggacgaa tttctcttag aatcaacgcc gctgctttat gtcttccttt
     3601 gaaaagatgt tcagccacac ccaatctggc agcaaagtat gcacctgcta tagaaggata
     3661 atgatctaaa ccattagcat tttcaaaatc tattccaact cctaaatttc cattattatc
     3721 ataccacgct tcttgcattt cgaaacccca tatgtcatct ggaattaaaa ttaccgaata
     3781 ataattactt aaatgagtat atctgtaaac cttaaagaaa tcgattgttg gaaatgcctc
     3841 gatctttttt atcaaactag ctgaaatgac gtcatcggta gcagagatac tccatcttgt
     3901 aggcaccaac cttcggtttt tttgcaatcc taacatcccc atgctgagaa ctctgtggat
     3961 tctactgact tctaccccac cttgataaag attgactata ccttccgccg cacgcaaatc
     4021 cttgtcataa aaaatatttt caagtctttg atctaccgag agtgaggatg aggttttaaa
     4081 ggtctttaaa gatgcaacag gaccaaaagg ggctgaatca gtatctaaac ccaaatcttt
     4141 ctcttgctct atatctgcga tgggtgtttt ttcaaaggtt gcttcacttt cgactgattt
     4201 actggtcata gctaactcct gcaaagattc tatgtatttt ccagacgtac tatgaatatc
     4261 aatatttgat actcctcgca caagagataa tcgataatta acaatttcct caatactttt
     4321 gtcgagccac atctctggct tatccagtat tgttgtgtct ccatgtaatg gtggcaccat
     4381 cggcccaact tttaccttag ggtaaccata acgtccaaca aatactgaag gtggcgtagc
     4441 accagaaagc gtctttagcg agaacttggc agaattattg ttaaggaatt taatccaatt
     4501 cttctccagt tgttccttga tttcgttagc agtaggcagt tcctaatcag tatgttaaag
     4561 cgccaaaaag ttatttgaag atatagcacg ccagaaacta ccagaacaca aagcaggctt
     4621 attcgaatca tactttatga taacgcaggt atctgcttta tgtatatgat taatacataa
     4681 gtataagatt tatttaaaac ctataaatca catattatat gaaatttaca gtgataacag
     4741 aggaataatg agtactacga accagtcaat gcaactacca atagatgaat gtgaggagtg
     4801 gatatgggat actttagaca gtacaaaaga ttgcggtttt gaatatataa aagatggaaa
     4861 taaagaattt cttatattca atacaaatga ttgcttcgca gatgctaatg ccaatagcga
     4921 aaatcaggaa aactaccggc atggaattgg ttcaaatgag tacacataat ggatatacac
     4981 agttatggtt tggtagatat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaag gtaaaactct cctaaattca
     5281 atgtcattaa tttcaaatgc agtcataaaa aagaccttaa cttcgggttt tatttcttta
     5341 acttttttta taaactcaaa tccattcatc tgtggcattc taatatcaga tataaccaga
     5401 cgatacttcg aagcatttac cttaaaatgt tccaatgcca aagctaggtc tgtaaatgca
     5461 taaacgtcaa acgaacggtg cttttgaaga cctaatttta ttaccgtgac gatatcaaag
     5521 tcatcatcga gtatcaatat tgaggattta cgatttctat cctcaacatc atcgccgacc
     5581 tccacacagc ttttacttag tgatggggaa tataaaagta attttctagc gctaaaatag
     5641 gttgtcgtta gtaatgcctc acatcatgat atattctagt tgtcagcacg tgtgggtttg
     5701 gatctgtcaa aaacggttaa atcaggagcc agaagtccta cgacaatccg ctggaaagat
     5761 agatcaagcc aaagattgat tctaggcgaa atatcattct acctaacatc taattttaac
     5821 tcttctgcat gatttctcct gtgacactga ggacacaata cttgacagtt agagggttta
     5881 ttattggatc tatcaccatt cttatgatga aagtctcttc cgtacagctc gagtttttct
     5941 ttgcagatgg cacacctgta cttctgctta cggaggacta ccgccttcac accacctgga
     6001 aaagaaaccc tattctgttt cttaatgcgt gctggtccac gatacaggat tgtcagagcg
     6061 atgaacaaaa gcgatattgc aatggctttt tgttgtcccg tagtcaaata atgaggaaga
     6121 gaatgcgaag acaaccatac gtaggttgat gcaatagtta gaagcatgcc aatcatagct
     6181 ctcaactttg tcttccgttt cgtccatctc cttatcccaa atattaccac cataaacaag
     6241 aacaatagat aaatgataac tgaataatct gaaggtagaa tagaaagtga ttccgtcata
     6301 agtgaaacgt gaaattattt tggaatgata taactcctga ggattctgtc tctataagat
     6361 attacatcaa tccccttcat tatttgtaga cctgcctcgc cagtctaatg tgtacgaaga
     6421 tttttcttcg gatgtatggt taaaccttgg aagatgattg catatttgtc actcacctaa
     6481 ttttctcttt ctaactcggg aaccatctct ttttcttcct caaatggcaa acaatctgca
     6541 aaacaatgcc attattggat ctctggtaat aatgtcttcg tctggaataa gactcgtagc
     6601 aatatcaata tcaaccagat tttttttcat tttatatttg aaaacagtat attcagctgt
     6661 ctctgattct ccatacatca ttgatttaac cttatgcaaa agcttttggt atttcttatc
     6721 acctgtgtaa aattcgtaca tctgttcgtt ggtatgtgag gctattgtat atgcggtttc
     6781 ttgtgggtta tctaagcttt cgatcgatag cagggcccta ctcactcaaa caagtagaag
     6841 atgccaagac gatggatgtg gtcatgaggc tgttggaacg aacgaaactc atgatgggca
     6901 gttatcgcac tcatgaagat ttacgatgac ctccaactag gtgatgcaga gaaatgacca
     6961 aacaacaaat ccttagatac tagatcatgt ctacgaacaa agatagagat acgtctatag
     7021 gaaggacaag ttgctgcaac tgaatatctc tattcgcagg gattatttgt gcattaattc
     7081 tgggtaacag tgcaataaac tgcaacaaaa taatgactaa aagtagtcaa aataatgact
     7141 acgacatcgg ctagacctac aacaagatac cactaacaca tttgcaatat tctatgttat
     7201 tgccatccaa aagtcatttt atacgcaata tctgctgcat tttgagagtc ttcattagct
     7261 actcctgaaa aagaagtgct ctgtccctgt gtaagactat cagcactagc aaaagcggga
     7321 accatgccca cgttctgacc cttattatct atcaacattg cattaatatc cagattagta
     7381 atatcatgcc cgcttgtatt cctcaatgta ccttctactt gaaactggtt tccataatgc
     7441 cctaaactga ccttcttgac tgtcccaaat gctacttgaa tgctttttac attcccgtct
     7501 gcaaagttat ctggtgtggt gtgcttgcgt attttgttaa cacttgatcc gcatatacca
     7561 gaggtactag tagaagtggt attataccaa ctagaaaaat gtgatccaaa ttaactacaa
     7621 gttattaata tattcatagt gggcattgca atatgtacac aataaaagcc aacggtgttg
     7681 cacaacgtga tttgtgaatc ggcagttgat gcttgcacct tgttggagtc ccatgaaatg
     7741 aggaatgacg atattgagaa tggttaatgc ctggaaggtt caaaagtaat gttacgactg
     7801 ggggattctt ctcctcgtcc ataatgacaa ctctttgact tgtgagttcc tggattagga
     7861 ctatatgcct atgaaagatt ccacgacgta tagagttgcg ccatattaca gtttcatatg
     7921 gttggctgtt acgtatcctt tcggcatatt aatgtcgttt ggcggtgttg ttggtgtggt
     7981 agtagtcatt atttatttaa tcctggcatc tattcggaca ataaatgagc aattctgtgt
     8041 caacttgcgt atgcgcacgg caattatcac aaaataacgg ctcaatacct tcatttgact
     8101 gataaaagaa taattgtttg ttttcattgc tatagacata acctccgttc ggtccgccta
     8161 agtacgctag taggttgtct ctaatcatat ctttgaacaa tacggcattc ttgaaataaa
     8221 ttgaaaatat acccaagtag tagataactt gcgcacgata gttattcgag atattcacct
     8281 catgcaccat actttggata tagtcacaaa tggtaatcac attgctctta ttttgcggta
     8341 gcactctgtt aaacaaaatg ttatagcatt caccaccaag tccgttagtc gcagtttgta
     8401 tctttcgatc tatatcagtt gtcgtcgcgg tcgtagtggc agtatgtctt ttaacgcgtt
     8461 atattatgag tttcattcat aaaaacataa gtgcatattc tcaaagtaat tagcagaagg
     8521 ttaaaggata taatgtccac tttcaagatg ggactttttt ttgcttctta ggcattcttg
     8581 tgcatataaa gtcaaatgta ttagaactaa gtctcagaag tatattctag atatttttca
     8641 attagattgg tatttaacgc ctttatatag tttcgtcata catcttttgt ggggggtgtt
     8701 tgacataaga catcgcaggt tagaatcaac ggttgctatt atgattctta ctgtctcaat
     8761 aagttggatt ttctcatcaa atgtaaatgc tctttatgca atgccggatc acagtactgg
     8821 taacgcaaca tcacaaccgc ttaccaaaga taatagcact acttccagtg ctccctctgt
     8881 cttacatttg tcctccgtcc catcaaataa tggaacttca tcaacatcag aaccttctat
     8941 caacaacaaa gccgacacaa attctgccac cggttcaaac agcgggacgg ctcacacgca
     9001 tcatggtact agcaccggca gcaccggcaa tcatcataac gataacacta aatcacatga
     9061 tcttgcccag aagataataa acaagattaa gcaaaaattc aaagtaggag atgttccttt
     9121 tccttgacag cttccataat attgtgtcac acctgcatgt agaataaatg gcttattaag
     9181 tcagcatagc tataacaatc catgctgcac gctcagtcgt atgtaatact caatggtagg
     9241 agatctctaa tttgcgatat ttctaaatat gaaatcaaat aactatttca agaaaggtaa
     9301 gcggccagat gaatcccaat cagagactac tatcattatg ttgccttcag atgccaatcc
     9361 aaaagggaat gttttcggtg gtaccatact aaaacatgta gatcttatcg caaatcttgt
     9421 tgctaataga catgcaacgc atgcgaacac tgtgactgca agtattgaca agatgacatt
     9481 tttaaaacca gtatatattg gaaatgcatt gatactttct gcaaggctta actatgcgaa
     9541 aagatcttct atggaagttg aagttacagt acacgcagaa gatcttgaca acggtaaacg
     9601 agttctaact gggactgcat ttgttaccgt agtagccctt gacgatgagg gaaaaccaac
     9661 tgaaattcct ccattgaact tagatagtga taatgataaa agacgtttcc gtgagggtga
     9721 atctagaatg ataaccagac taaaagatgc aggtagagta tggacatcta caagtcgaag
     9781 tgaataatag aatctgtacc agaaactctg gggtatagta tgatgaattg gtacaatata
     9841 ctaattgaaa ataaaaggtt cattataacg cagagaatgg aatagacatc agtagaagca
     9901 tacaatattg tttctagtga acccacaggc aactttatct cacaatcgct cgacaatcag
     9961 atatacaatg atgcaatatc aaagagtggc ccagggtttt ctaataacaa aataagaatg
    10021 gatagaatat atgcttatat agagcatttt gttttcatgt ctcatgggaa taagtactag
    10081 tatttctact cgtacctgta gtaccgactc atttttggtt gaaagaaaga tggaagccgc
    10141 agcagacgga ctatccttgg attgtcttaa tctgctattc aagaaagtct tcccttactc
    10201 tccgactaat gctaagacaa ttgccgatta tgtcctcgca ttaagagccg aaaccaaccc
    10261 agcggatcat tatcgaacag atatcataca cttgctcaca accttttcaa tgtttttcaa
    10321 aaatcaaaag acattcatag aaattagtag ggacgatttg ttggccttcc ttgatagctt
    10381 caggaaggca gaaagtgttg atccattgca taggtggatc gggacttaca atgtctacag
    10441 aattcatttg atgcgtttct tcaagtggct atactatcca gatattgagt agaaacaaag
    10501 atcaaagtct gccattatcg agaatattcc ccagcttaag cggaaggaga aatcagtata
    10561 caaaccaaca gacttgtgga ccgaggaaga cgattcgctc tttttgaagt actgtccgag
    10621 tcctcgtgat agatgttatc acacaatgtc acgtgattcg gcagccaggc cacatgagct
    10681 tttgaagctc aggatcaggg acgtcgtatt caagattgcg ccggataaga aacaatatgc
    10741 ggaactggtt gtcaatggta agacaggaag ccggtcaatt ccgcttatat gctcaatacc
    10801 gtatttgaaa gattggataa ggcagcatcc ccaaagtggg aatcctaata gcatattgct
    10861 ttgcggatta ggcaagagca ttggtagagt gataaatgta atgagtctta acagatttta
    10921 cgaaaaatac aaggagaatt ttttcccaag gctactggaa agcccaaacg taccaccaga
    10981 ggataagcaa aaaattggag agctgttgaa aaagccatgg aatccctata ttaggagaca
    11041 cagtgcgctg actgagaaat ctggtattct caaggagcat tatctacgac aatatgcggg
    11101 ttggtctcca agctcacaaa tgcatctcaa atatttacat tattacggcg acgagagctc
    11161 tggaaatatc ctagaagcgt atgggattat aaccagaaat aagcaactat caaacgtcct
    11221 caaaccgaaa gagtgcccca attgtagtga accgaacaaa ccagataacc gattttgtgc
    11281 taaatgtaga atggttttaa cgtacgatac atataatgag actttagaaa atcagaaaga
    11341 aaaagaatcc gaagtgcaga aattgcaaga aaaatatgaa caagacatga aagtgatgcg
    11401 tgaagaaatg aatcaacagc taagtgagat catatcgatg ataaaacaga atccagcgct
    11461 tgcacacgtg aaatccgatg tattaatggc caaaaaagtg gtatgagaag tgaagctgca
    11521 aaggagcaca aatacctggc aaatgacggc tagaagtctg cgaagaaatg aatcaacagg
    11581 ttaatgagat caccgatgtg aaacaaacca gcctaaagac aggcaagacc aggaaatgta
    11641 atattatcta tgaagtcgat aagattttat acattaagtc ataatgattt ttatatacat
    11701 tccggctcca agaggagcgt cgcatggatt taggatatag gatttctaag aatggctgaa
    11761 tcagatgttt gtacttatta gtaatatttg cattagatct catcaaatct ctaaatttct
    11821 cgtctatttc acttagtacc tgttcaatga cttctttagt caattggatc tcttttcttt
    11881 cttttttctt aaacgacatc cgttcaaaga atgaattctt tgcaacgacg tccacctttt
    11941 tcatgtatat ctcctccaac cacaggcgct cttccgttgt aggcttggaa atatattccc
    12001 attttgctat cacccagtca attaagtctt tgtacaagtc cgcgcaacat gtcaaaaacc
    12061 gttgtattga tgggtctgaa aggacatatc tcatttcatt atcactcatt gtgagtatct
    12121 tgagaaggcc gtaatctcgt aacaaatcga tagatctttg aatctcagcc tccgaaaaaa
    12181 caagatgtcg aaattctaga ctttttgaat ttaagatctc cgatacactg aacccgggaa
    12241 tgtttcgaga ggcatggaaa aggactagaa aaaacgactt tagctgtttt tcctcttgtg
    12301 atagtgtccc gtctgcattg gagtcaaatc ttaagattcc tatcttttgc tgctgcttag
    12361 catcttcagt catgctgtat tcgacagccc ctctaacgcc attttcaaat ctctctatta
    12421 tattgcggga catcaatttt ttcttatgag aagaatacgt cgttggataa atttttttgt
    12481 acgatttgca tatgtgctcg caaatttctg catcagggac aggtttttgt ttatctaata
    12541 gtagaccaac gatgcaccga tcgatctcgt cgtcacgtct catcaaaaca tctgtagtac
    12601 tacacaatat gctcttataa aatttatgaa gaatgtaggt atacaagaat caattgcggt
    12661 tggcatttca ttgccaaaga aaataatgtc acagatagat tctgagcgcg gtgacgtatc
    12721 cagaagtcga tttttattaa gactaattga gagacaaagt atagtatcga acgtagaaac
    12781 aaaaaacgtg aatgatccgc ttgaccacgg aattgagacc cctagatcaa gcgaatcacg
    12841 tgactaatag gagagattat tcgcctatgt ctaagcctac tttatcttta gggcctaata
    12901 aacaaatatg tgaagcagaa ggatgttttg cgacagcaga agaagaaatc aaaatttctg
    12961 tgggccgaat cggaaaaatt aatctgagcg tatgtgctaa atgcaaaccc aaattcatca
    13021 ttaatagcga aattactcgt gaggaaatcc gggagcattc gaattgaccg tacaaccgga
    13081 attgtggaca accgagaaat cgatcgacga attattcctc catcttatgc gtatccaaga
    13141 aattgtgacc aacgataatg gagataaaat tactgtcaga aaatacccca ggctcgcagc
    13201 agaattagga agatatatac ccatagctaa accgacgcca gaatacatta tagacaaatc
    13261 agatctcaac gataggctaa gaaaggaatt actggaggaa attacatcag aggaggcgcg
    13321 ccaatatgtt ctgaaagagg ctgttggccg aatcttcgag aatctgaatt ttgaaaggta
    13381 ccatctaagt atagcgagga atcttaaagt caggctggtt tagaatgatt gaaatttcca
    13441 ctaatgttat tcgccaagga tacctgataa ttgtagaagg cttgaattca agcgtcacat
    13501 tctatcccaa tgacaaagac aaaacattcg gtgccctaat tggtgcacta aaggaattag
    13561 gctcggattt gtccccagat gagattcata atttggatgt ccaagtcaca aacatctaca
    13621 aagaatatta cgaacaaaga gctaataaga agacctccga ctctgaacat ttgtttctat
    13681 tggtctcaag tgaaattaaa gaacaattca cagatcaaac cggcgcgttt tatgcagcta
    13741 ttgaaagaga cggccacaga gaaattctca acatggatta cgaagaattt gatctattct
    13801 taagcaatat attttacaag tctgaaaata aagtcctaag taaagataca tccaataata
    13861 ccaaaagatt gttgaaatcg tttactaaaa agaaaagaac cctgcataat agagtcgcaa
    13921 aaattgtcga tacaatatac tatgatctct gcgatgaaca ttggaaatgt gttaagataa
    13981 ccaaacaagg ctgggagatc attgcaaatc
//
LOCUS       DAOR01000219           14456 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21803, whole genome shotgun sequence.
ACCESSION   DAOR01000219 DAOR01000000
VERSION     DAOR01000219.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21803"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    332..702
                     /estimated_length=371
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9817..9910
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10179..10441
                     /estimated_length=263
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10671..10785
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4275 a         2564 c         2692 g         4082 t
ORIGIN      
        1 tgttgccttc ttttggatcg gcgatcctgg acaatccatg ctcggactat ggtcactagg
       61 cgtggcacct ctaggcctca ctctgcacct tgcgtttgat gtggccttaa acgatctctt
      121 ggatgctccc aagcgcctga aaacgaaggg aattaaacca ctctccttct tcggtgtaga
      181 gacaacggag ccgagcgtga tcggctggat gccagcagcc tcggtctact tccgagatcc
      241 cgacggtcac acgctggagt acttgactat gcttgacaaa gagcctagac aggatttagg
      301 gattatccct tggtccgagt ggctagatca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattgttgc cgcagattca
      721 gaccgcatcc tattcagata ttcatacaca tttagtactt cttgatctga tatgccatat
      781 ctttgtagct ttagtaaaga tgttatcgct ttgtttcgtg gcgcgcaaat ttcattcatt
      841 ccaaatattt gcacggctag tcgggttatt tccttattca tcccgccaat tctgttataa
      901 ttctcgatgt cctcaattac cctgtaagct gctgccgaaa tgggcaaatt acatgtcctt
      961 gctttttcgt ttaccgcaag actgaatggt agtaatttat cgatgccaat tcccatagac
     1021 cgaattcgtt gtaagagtgg tattacttgt ctatctcctg acatccgttt ttcgagcatt
     1081 gcgcaattgt ccttcaatgc tttctctctt tgttttagcg atttgatatt agagaattct
     1141 gccacaattt tcttaggatc ataccgataa tccttgaggt tatcaagtac tgttagaagc
     1201 ctttcagggt catcagagga gagatgatac ttacttaatt ccgcctttaa ttgcttgtac
     1261 tcgttaattg tttccatttc tacgtttgta ctttctaaaa tcgcgcgtgt ctgtttgatt
     1321 tcttcttcaa gtctttgctt ttcttcctcc ttttgtttga catgtccctc caggtcttca
     1381 agcggtattg attcagatct tgacaaatgc gcgacttgat tagcgacatc aatcagcttt
     1441 tcgggttcgg gtgaattggc caaattagca atgaatgatt cgatttggtc ttgattcgcg
     1501 cccaattttt cgatataatt tcttagcctg acagatgaag catgctcacc cagattcagt
     1561 ccttgctttt tcgaaaatac tgctaattct ctgatagtct catattctga atctgcgagt
     1621 ccttttttcc agtcattgct ttgaattgta atacgttaac cacggccttt cttcggatat
     1681 attgttcggc tacctttcga atgaatgtac ttcttgggat atcgcctcgc gctttttata
     1741 ttttttgtaa gatcgattca gggggaggta atgcccactt tatctgtctt attatgaatt
     1801 gttgtactag tcatactagt acaatatatt gttccgcgct acatttaaac atatcgtttg
     1861 gcaatgtaat tttgttctac atcgttcctc cagccgcagt cagtcgctaa accgacttgc
     1921 tgtaccttgg atgaataatc gtctattgag tctacgccta tgaaattggt gataccgtgt
     1981 tgtgggtttt gtcttttcat catccctcca cgtaatgctt cgtttccctt gtcgtctgta
     2041 gtagttatca tccaatattc catagtagaa tcacttccag accacttgtc tatcttccat
     2101 ccaaaaagtt catgatagaa cttctttgat ctttcaatat tgtcagacgg aatttcaaag
     2161 tgaactattg ttgcgatata cttatcttca catctattca acactttaaa aagtaagata
     2221 gtaatgatct gtcgacatgc ttgtcaataa caagtaatta cttcataaca tcccgctcat
     2281 ggagcccagg gtgtgtgaaa cgttatacta cgagatactt tctgaatttg ctaactaata
     2341 aatttccttc tgtttatatc taggatgaca ataatgaaac tataatgtct ctctctgttt
     2401 attcctaatg acgaatccaa aagaaaacaa caacatttga tgactgattg gtctttctta
     2461 tctaagggct tcagcatgat cttttgcgaa ctgaccaaat aatattggtt ttcgtcctgt
     2521 aatctgttca actacagtag tcatttgcgg tccatgacca gctctaataa tattgttgaa
     2581 ctccatcata gcatcaataa accaattttc catacccatc tctttcattc ccctgcgagc
     2641 atcttcttct ggtatgtcta tatatgatac tcttttacca atctccttag aaaggatttc
     2701 ggctgcttga ctaaatgata atgtctcccg gccagtgata tcgtatgctt tattctcatg
     2761 ctgacttcta tttttagtta gtacgttcac agcaacagct gcgatatctc taacatcgac
     2821 gaaactaacc tttccatctc ctgcaggcag gtaaaaagta ttttgatttt ttattgtctg
     2881 gccaaaaata tttaggaagt tttgtataaa tgcagtcggg cgtaggaagg tatacggtat
     2941 tccagattcc tctatgattt tttcttcttg tctatgtaat cgtccaatta taattccagg
     3001 ttcagcatcg gctcccatga cagataactt cactacatat ccgacaccat tttctttgct
     3061 tcttttacca aattagaaga aacttgtgtc atgttaggcg taggtagtgt caccaagaag
     3121 agtttatcaa cattgtatag agcaccaaca atagtttctt gtctattgta atcgatgtta
     3181 actatttcaa ctgctttgtc aaccttaaat ttatcagctt tatattgcga atgaacggct
     3241 gctctaacac tttgaactaa aggtgttagc tgctttacta cttcgcttcc aacagtacca
     3301 gttgcacctg ttactaaaat tgttctccgc cattattcat aattatgtga caggattgaa
     3361 tctatttaaa tttggtagta atttctaact aacttgataa ttcatttatc agtcaggcaa
     3421 tcttcttgct aaatttggat tgatcaagct gattaattat atctgtggct atgagtattt
     3481 cttttataca ttgtaaaatg gaaggtctca tgcacgccta taatttgcac taagcagcgg
     3541 tatatctcct ttagttcatt ttctaattca gagttatttt ttcttgacgt attgaactaa
     3601 aaggtactat acttggcctt gtggatgaaa aatggtacta tgcagccgca agttcagatg
     3661 aaacgaacgg tatatccaat catatttgct aataccaaat aattcgtaga acagcaacaa
     3721 ttattctttg atactctggc agaagcaatt cccgcagaca gaggatcaaa gatgttaaag
     3781 aagatttaaa gagagaattt attgatacaa ttagagatcc tgctgagata cagtctatag
     3841 ttcctagagt catatcctct gctgctgaag agctcggtta ccattcctac cgtgaatact
     3901 ttttatcgta tgagagagag tattagaaat ttttgaatcg aatgtttttc caagaatcaa
     3961 aactgatcgg tctgttgatt ctgcctcgtt aggattttac ttcactattg acgtcctcaa
     4021 atgcaatctc gaaaactaag aagaatataa tgtcgatagt tgttcatgtg atttcatgac
     4081 ctcttcgact tcttagaacc tgtcaatgag aaacaatgat ataacataaa gtaataaaca
     4141 tcgttggaaa ctgaagctct aatcagtaga aaatataagg ggaggccaga ttattgaaaa
     4201 ataaaaatat tatagcttac ggggtaattg gaattattgt tgtcgttgta actgcttacg
     4261 ttgtttttat tagacctcca aataataata ttagtaataa taatgttatc agcagcaaca
     4321 atccaaactt gcagtctact gcccaactta tcgcaaattc aagagctgca gcagttgaac
     4381 aatatcaaaa gctattctgt gggttaaatt ctattccgca ttctactaat tacataacag
     4441 agtacaaact cccagctaca tgcgaaatgc ccctaggtgt cttggttgac gatcaacttg
     4501 caaaggtatg gtacatatct acgaaacatg gaacgctcgg tagctacgat cttgccactc
     4561 acaaatttga taaagaactc accattcctg tgtggaacgt acgcaacatt cctactgaca
     4621 attctcaggt atggtctgtt agggaggata agagtggaaa catttggttt acagacgaga
     4681 aacaaaacgc aatttggagg tatagtaagt ctgttggggg ctttgaattg tataaagttc
     4741 ctgagatgtc taaagtcttt ggtacaactt atcctgtatc tgttgatttt gatttaaaag
     4801 gaaatgtata ttttgtgggc atacgtatgc ctgtactctg gttcggaaac actacccaga
     4861 tgaagaatgg tacctcaaat ggaataaata agattccaat acctacagat ggattcaagg
     4921 gtatatctcc tgatctaatc agtaccggtt ctatcgcagt cgacaataaa cggaacgtcg
     4981 tctggatttc aatgttatct ttccctagta agggcgaaat tctgcgatat aatataacct
     5041 caaaaacttt tgatactttc aatttgccgt caggattaaa ctctccagta ggagttgcag
     5101 tagacgagaa cggtaatcct tgggtcaccg atcatgctac gagtctattt ttcatgttaa
     5161 acgcctccaa cggcaagacc gcaacctacg caacgtctag agtttcgcca cagatttatg
     5221 gtgtaagtga cgttaatagt attccacaag gtgcttatag tctgccttac tggatagaaa
     5281 aagggtctgc tggtagcgat ggatcgcttt ggtttaatga gcatgaggga aacaagatag
     5341 ccaagttcga gccttcaaat aataccttgt acgaatattg gattcctaca caggctagat
     5401 tgtggggtaa ctgtcctcct aacagtcaaa catgcggtat agccaacacc ctacagttct
     5461 caagaggaca gaatggccag acatggttta cagaatggtc agaaaataag atagggagcc
     5521 taacagcaac caattacaga cagctaccat tctctgtttc agtatatcct caagagctga
     5581 ctattaaaag aggtcaggct gtagagatta aagttaatgt ttcagcaacg acaacaacaa
     5641 gactgtctca gaatcactcc aactcaactg ttgaaatggt cgcatcaggc acatttactc
     5701 caggtggtga cctcggaaat tccaccggca tcttcagcca agggtccttc tcgataccag
     5761 atgctcatgc tattaaacaa gtgtcgttca tcttcacatc agcaacaaat ctaaagccag
     5821 gagaatatgt gctgatgcta ggagcgcaaa acaatgaagt tgcgatgttg aaggctgtaa
     5881 aagtgcacgt tattagctag ctatagtcgc tactgctact gcgggtatca agctgaccgt
     5941 gatacgctca atcatcgcgg cgagatatat gtacatctat ttcatatgta ccgccgtcat
     6001 tctattccag ccgccataag ccaaatgata ccctagaacg tactcctatg aattatgtcc
     6061 agatggttct gaatgggtcc accgcattgc acaccactaa gtcgtccatt gcggcaggag
     6121 ccgtcgttat atccagcggc acgtgtgtgc agttatggga tagatgagtt ttgtactatt
     6181 ttgctactgg tagtggcgtt agcaaatgat gcgtggcaat tactgttgat acacacgact
     6241 gtatgacatg tatctccaac acagctagta gtgctctttt ttatatgaat ttgtgctttc
     6301 gctaaatatg tatgctcccc taatgcaatg attaacatag atgctatgac aaccattgtc
     6361 agactcaaaa ttgtgtgatt tatcttcatc ctgagagcaa ttgataacat aaaatatttg
     6421 atttgggagg aatattgcta tatctttact ctaatagatt tatcagaata aagcatcaat
     6481 taaagatagc gcttcgagta tatgaaaaat atctatttat cacaacaaat aaccatcgtc
     6541 acataacaga agaaggaaaa tctctcaatt atttgcaggt accgcggtag cgttgttctc
     6601 gatagaatca agcattctat aataagttag tataaagaga tagttataaa aaggtaagcg
     6661 gcacgatggc aaaaaatatc gccccgttca ttgcaccact ggaagttcca gcaacaatat
     6721 caaataattt gtcgaatcct tcttttcgac ctattttttc atataatgct tgaaagactc
     6781 ctgtatcata tgctccgagt gcgcctcctc cttgaaaaac aagtgccttc ttctttaatg
     6841 ttgatgctgt gaacacatac tggccagaaa ctttgcacta cgccatgctt atgatccctg
     6901 ccatccttgc caatttcgtc aatcataact ggattatcaa ccaattgcaa gattaatgta
     6961 atgatggtaa ggatcaaagt ttttgaattc agattatctc ctcagtgaat gaaatacgtc
     7021 atacgagcct gttttggaga atgctgcagc tccaggagta tacgatttaa gacacataga
     7081 catagaaaat ctcgtgtatg cacgtcaata tatcggaggc ttgaactgaa tgtttgttta
     7141 aagtcaccta ccactacata atcaacaaag attcatgcat ccaaaattag tactccatta
     7201 tgagttcaaa ttaatacatt cgacataacc caactaacca cgctattcga ctttcattaa
     7261 ctggcggcgt tacttcctca tctaattgtt gttggtttta ttgaaccccg tgttatttat
     7321 cgatatttta aaggagtctt tcctactttg aactattggc aaatactacg aaaatttttc
     7381 tatatgcggg ataatctatg aagtaacctt tgcttctgtg tgctgctgta ccaaattcta
     7441 ttagttttct cattccatat gtcggatgct tgtacgtcca aataatttca tatgtcggaa
     7501 ctcctgcaag actgaaatta ttatttttgg ctgttgcttc aatgagttta aaatcaggaa
     7561 atgccgatga atttttcttc gattgtaatg atttgttgaa atactgttgt aaagtaaaag
     7621 catattgctt aaactgcttc gataaattac tattaaccca tacataagaa aaatggaagt
     7681 ccatctatta ggtagataaa aggagcaaat aacttgtgga tgggcaaaac taaaggtgga
     7741 tggcaatgag taattactat taattctgat aatccaatca tatggatatt gaactttgag
     7801 tccatatggt tcattgtcat attccaaaat ggtcatatag gaaatgtact attagtagca
     7861 gattctcgac acgtgaagag ctagcagttg ggtaatgacc tagttgttct tctaatgtgt
     7921 ttaggtacgg tattgaacta ttcttagaaa caacactatt cttagtattt gtattagagg
     7981 tttattccag tgaaaagcct gtcagagtag aataccagcc tactgtaaaa ggtttagcgt
     8041 tgcaacctat tatggatatg atggctgcgt actctatgaa gtattgtagt aaggatgttt
     8101 tcaaagatgc aaagcccaga gaattccaag aagtatatgg aagagaaatg gctaaagttt
     8161 catcctatgc ctgaaattcc atagttcatc tacaacatcc gtatagttcg gatgctgtta
     8221 cttctttctg taaccaccat catcaaccaa catcaagcta tgtgcttggc tgtcatgctg
     8281 atgcggttga taaatccgag atattctgta attacaagat agaattggga tcgtcacatg
     8341 tgactaggtt tgttcattct tagtcaacaa atagcgtatc taaccatata cattgtgatc
     8401 aataaaaaat atgaacgaaa ataggattcc gcataccaaa aacgcactag tgtcattttc
     8461 tgaggcaata gagaatgtag ccgataaggt atcacaatcc gtagtaagtg tacgttcaaa
     8521 gactagagga catggttcag gagttgtttg gacttcagat ggcctcatag tgacttgcag
     8581 tcatatagtc agaaaactag acgaacttga agttagtctc agcaatggta agtcctttcc
     8641 agccaaggtt ataggaaatg atccatattc tgatatcgca ctcctgaaaa ttcaagccaa
     8701 tggtaagaat gatagcattt ccctaaatcc aatagagatt ggtgattctg aaaatctgaa
     8761 agcagggcag tttgttctcg ctcttgctaa tccctatgga gaatatccca gcattacgga
     8821 aggaataatc acaagcgaaa gaagctcact tggaggaggg cggtggggag gaataacgaa
     8881 taatatagtt atcactgatg cacgtctcaa cccaggatat tcaggcggtc ctttagttga
     8941 cgtagaagga aagatgatag gattaaacgc agcatatgtt tcgtctcgcg gtattgcaat
     9001 tagagcaagt aaagtaagaa atatcaccga ccaacttgcg aagtatggag ctattaagat
     9061 agcatacctt ggtgtggtca cagatgaaat ttctcttcct agagaggtag gagctcaact
     9121 aaagccaagt caagaagaag gcctcatggt tctttcggtg gaaaatgaca ctcctgcaaa
     9181 gaaagcaggg ctattactag gagatattat cataggattc gacgatcagc ctatagcgaa
     9241 cattcatgac ctaagaagac aactcttaaa tcaagatgtc atcggaaaat cagtaaagct
     9301 tgccataata agaggtgaga aaaagagtga gatcacaatt actcctcgag aatcatctgg
     9361 atccaactaa ctgcaattaa agtcaagcga aagaggaagt aataatataa tatatgatac
     9421 cagttaatga agatctaatt gttaatgcag ttcaaaaggt aagtagaagt gttgtaaata
     9481 ttgcgagtgt aagaacagta catgatcaac tatttcgagt atttccggtt gaaggtgttg
     9541 gctcgggtgt aataatcgac gagagatgcc acgtacttac caataatcat gttgttgacg
     9601 aagctcagaa actgaaaatt acactcactg atggaagcat cttcgatgga aaagtgatag
     9661 gaactgacga gacaacagat ttggcagtag taaaattaga ttcaacagaa acacttccat
     9721 tcgctgcatt gggaaattct gatgatttga agataggtca aatagtgatt gccattggta
     9781 atccatttgc tttaactgga gggccaactg taactcnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn aactgtaact gctggaattg taagctcatt aaacagaaag atccaatttg
     9961 aaaaaggggt actagaatta attcaaactg atgcagcaat aaatccaggt aactctggtg
    10021 gacctcttat caatacaaaa ggcgaggtta tagcaattaa cacagcaaaa atgccatatg
    10081 cacacggtat tggatttgca gtaccaacca acattgctaa atcagtaatg acagaactaa
    10141 tacaaaatgg aagagtaatc aatagaccat ggattggann nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nacgacaatt agctcaatat tatagattgc cagccacaga aggtgtgtta atagcacagg
    10501 tagaatcaca cagcccagct gatgatgcag gattgagaaa gggagatata atcgaagcag
    10561 tggacggcaa gagaatagat gataccacgg aaatttcact aaatgtgaga aagaagacac
    10621 taaaggacaa gctaacaatg actgtaaaca gatatggaag acgctttgaa nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacag atatggaaga
    10801 cgctttgaag ttccaataca acttcaggcg cgaccatgag tcttattatt atttttattc
    10861 tatttgtatt cgcaggttac gacttactta actaatgaaa aattacctcc aggggtgatt
    10921 tttcgtttat caatttgcaa aaataacata gttgttattt tttgatttgt ttgttagaga
    10981 aatagcagat ctgagtaata tcacgaagct ttacgcgtag tcatatgagc cagtttgata
    11041 tttgggagta acaggatcta aatgtggtag tatgtttata ctacttgacg tgactaacct
    11101 cgataaatgc tagtcaatta atgccccatc cttctatata ctatcaaaga atgacaacaa
    11161 tagttggtat caaaacaaag gaaggcgttg tactcggctc agatagacga gcaagtaaag
    11221 gcttctttat aggttcaaag ataacacaaa aaataacaaa aatagatgat actttagcag
    11281 tagcaatagc tgggcagtta tctgatgctg aatatatgat taaggttgca aaagctgaga
    11341 gaagactaat ggaacttaga agaggcttcc cactcacagt gaaggaatct gcgaggctaa
    11401 tagcaaatct agcgtattct ggtcttaaga actatcagcc atatttcgta gaattattga
    11461 tggcaggcgt agaccaagaa ggtgggcata tattggcagc agatatgagc ggtgcaataa
    11521 ccgatgaaga ctttgcatct tcgggttctg gttctccaat agcatatggt gttctcgaaa
    11581 gcgtttacac taaggacata actaatgaac aagcaaaaca aattgccact aaagctgtag
    11641 cagcagcaat ggaacgagac cctggatcag gaaacggtat agacgtacta gtgattccaa
    11701 acttgttaat ggtcaaagag gagaagaagg gggtgagtca ttagaaatgg cagaaagtgg
    11761 tgctactgct agatatcctt actattatgg tcctgagggt agattagtat tagtagatag
    11821 tgcgttagag gcagtaagca gaggttcaac tacaatcgga attaaaactc cagtctttgc
    11881 cttgatctct agccacataa agccaaccaa gcccttagta gaacctgctg aaaaaatatt
    11941 tacaattgac ggacatatag gcgcgacagg ctcaggatat attggtgaca tattgcaact
    12001 aattgatgag atacaattag cagcacaaaa acatagactc acctatgaaa gccccatcga
    12061 tgtaaataca cttgcaaaac atctcagctc atacttgcac aactatacta tctatgccgt
    12121 tagaccacaa gcggcctcaa taataatcgc gggaatagat caaactggag ttcagctata
    12181 tcaagtagat ccaagtggga cattcttcag aggagcaggc tttgcaatcg gtcagtattc
    12241 agatattgca ctcgacgtga tgcagagaga gtatagcgta gacatgagta tggagcaagc
    12301 catacagttg agtaataagg caatagaaaa agcattggga gaaagaccac tggtagaaac
    12361 aggaatcatt acagctaaag acggttcctt caagaagctg gtgaataatt aatctagttc
    12421 tcacaataat acgtgaaacc gcttgagtag cggtacctgc ctaggaaggc tcgatagata
    12481 aaacccctct ataggtacga cgaattagag tataaacaca gatgaccaga gaaaattcgg
    12541 gtcaagatta ttggctgatg aaagtgggtc atagagcggt gaattagggt gatgggctcg
    12601 gcttaagatt ggaaattcaa attatgtcca gcgggaatag aaaacctctg tatcagaatt
    12661 cctacaattt ggtccgttaa gtcgaaatta ttgcagttac aaacaaggat cttaataaca
    12721 ctcattaatc agagcagaag ggacactttc tgagtttgtt ataatgcatc tttatattct
    12781 agccgaagta aacaggtgaa agaaacttat gacaatcgcc tcttcaatag gatatccacc
    12841 tggacctcac tcaattttac ctaacaaatt attgcgtgga tttatgcatg accctatcaa
    12901 gaccttggtg gatattgcac gtacctatgg agatatctgc cattttaaat ttggaagaca
    12961 acatatctat ctattgaata atccccatta cattgaagat gtattaatta gaaattataa
    13021 aaattttata aagagtagag gactacaagt atccaagcgt cttttaggcg agggattagt
    13081 gactagtgag ggagaatacc acgatcgtca aaggcgatta attcaaccta catttcatcc
    13141 caatcgcata aagggatacg gaaacatcat gaccagctat gcagtacgta tgtgtcagcg
    13201 atgggaggat ggaattacgt tggatataca taaggaaatg atgcatgtaa cgttagaaat
    13261 cattagtaag gccgtcttag gttcggatat taaaccagaa gatgacgaag ttggtattgc
    13321 attgcaaact tgcatggaat actttaatcg ccttcaaatg cctttcggcg agctgattga
    13381 aaaaatacct attctaccta ttaacaaagg ttatcaaatt gcaaaaaaga aattagattc
    13441 gatagtatat agtatgatta aggagcacag ggataaggag agtaagggtg tataccatga
    13501 ggaggactta ctatatacgt tactacaagc tcaagatact gaagctggaa tcggaagaat
    13561 gactgattca caattaaaag atgaagtaat gacaattttt ctggcaggtc atgaaacgac
    13621 atccaatgca ctcacatgga cattttatct cttgtcgcag catccaactg cagaggctcg
    13681 cctcaatact gaactctgtt ctgtacttgg tgataaagat gccaaaagaa ttccaactgt
    13741 tgaagatata ccaaagttgg aatatgctga aaaggtgttt agagaatcaa tgaggcttta
    13801 tccaccagcc tggactatag gccgccaagc tataactgat tacaaggttg acaaatatgt
    13861 gatacctgct ggctctatca ttttaatgag tcaatacgta atgcagcatg attctcgcta
    13921 cttttctgac ccagatctat tttatccaga tcgttggacc aaagaagcaa agttgcaact
    13981 ccctcgattt agctatttcc catttggagg gggtatcaga ggctgcgtag gtgagccgtt
    14041 tgcatggatg gaaggaattc ttctaatagc tacattatgc cagcagtgga agatgcatca
    14101 tgacccagac cataaagttg gattaaaacc acttattaca ttacgtccaa aatatggaat
    14161 gcgtatgaat ttagagcata gaaaataaga aaatcactag cttttcgaaa cgaaggaatt
    14221 gttctaccca ttaagcgaaa ttatttcgag gaattgctaa caaatcatta ttactataat
    14281 agttgctacc actctaaagt taagttaatt ccgggtattg taataacatc acttattcaa
    14341 ataaagagga aaattacatg tcattcataa taaaggataa accaaatcca aacaacaaat
    14401 tgtttgatat gacactatca gaatatgttt agaatatcat tattgcggca cttatc
//
LOCUS       DAOR01000220            7376 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35940, whole genome shotgun sequence.
ACCESSION   DAOR01000220 DAOR01000000
VERSION     DAOR01000220.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35940"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2274 a         1414 c         1326 g         2362 t
ORIGIN      
        1 atcaaattga ttagcttgat tcactggtaa ttccttttta tacatattat atttaataga
       61 atctactata aaaagaccaa gttcggtgcg gatctatcta tcatggtcga tctcataaat
      121 gatcatgatc tcaggctttg ttgttgctgt tgtgtctgag tgttttgcaa caccaggtta
      181 tcaaaatatt ggtcaaattg cgctgttgtc gcaatttctc tttagcttct cttctccttt
      241 aattgccaac agaaatgctg atacattacc tatgccctct accaatctaa gactgcatac
      301 gatatgttcg atctattgtg ttaggagcat ttgtgttaac ggccaccgaa atacagcaac
      361 tgtcctcaac acccgacatt acagccaagc tgctaatttg atatcatgta tcatatcgct
      421 agtatatcac tagcgactta ataagtgagc tgacttgtat aatttgcaag tttgacctag
      481 taagttcact ttctcgataa cattataata acaaaatact actattttgc atattgcgct
      541 ttgatatcaa gcgacgcatt atttatatgg tcttgggtat gatcgttttt ttaattgcct
      601 attctgctgg tgcagcagtt catatgggta agcagcaaac agcggactta agacaacatt
      661 ttgcccaaca aattagaggc attgatcaaa acggaatttt catcaacaac gccaagattg
      721 cgctcgggat gtttgtgcct gcagcaggga taggactggg agtaatttct ggtttttata
      781 cagggatgat atttactgcg atagcgcaaa cgtctactct taataatgtt cctcctctga
      841 taattctaat tactccgttt gggattatgg aagtttttgc ttatggtata gcaatatcca
      901 gaagcggcat attcatttat caacttgtaa agaaaaagcc atggagagaa tacgctatcc
      961 caatccttat tgaggttgga attgttatat tagtattatt ggttggagca gtaattgaat
     1021 ggcagattat aacacaattc agtggccctc acactctacc aaatgtataa ttcaggagtc
     1081 cggctagatg gccgaactat attcatagat cgtgacattt tagattaaaa tgacaaataa
     1141 acataaaaaa gtatgtaaag tgtattcata ttcgagtctc ttcacaagca ggacaattac
     1201 gcgctaactt cgacatccgt gcaccacact cgatacaata ttttgtagaa gaaccgctag
     1261 tcatgccaac agatttattt ttttatcgac ataatatttc aagcttaact atatatttaa
     1321 atgcacttgt gaaatccgta ataataagaa gtgttttaga tttaaaattt tttcatgata
     1381 gtaataggta cagaaaatat tcttacataa tagactctat ttttgagaac ggatctagac
     1441 tatggattaa tgaagcacat aatttacttt ttcaaggcat aattaaaaac agtagaaata
     1501 taacatcgaa tcaagatttg agactggcgt tgacgttatt ggtggatagt ggtttgcttt
     1561 tgtcactgga ggagtctggt cacaggtcac aataggtatc taatagcaga tcctgcatta
     1621 cacaatctcc taaaggaaat catcgacttt agaaaaaccg tacaacaaaa tgaaggaaaa
     1681 agtgaaacaa gacagattga ggagtctgaa ttgatgagga caaaaaaata aaactaatag
     1741 ccgaatcatt gctattactg aaataaggaa aaaacatgca atcacattga acgaattctc
     1801 gtttctatcc gacgttatcc aaatattttg ccctcctctc tttccagaat taccgtatag
     1861 agaattaacg atcctgcgag aatagaattt ctgatcaata ataactcaac caaataaaaa
     1921 gctaatttag aaaccaaaaa cctaatttgt gataacttct tctttatagg tgcctctggt
     1981 ttaacttggg tcagctgcca aattcatttt cacttccaat aataagtaat cgtcattttt
     2041 agatgatgcc tatgcaagta gtggtggtgt taataattcg gttatcgatt tgtttgaagg
     2101 gctggtcata acgtgtgtgt ttatctaacg aagttaataa tactctgtat gagacaatat
     2161 tatttcatga aaatgtttta tatacaattg ttttttttaa acttcatgac atgtacaaat
     2221 tctcatgcaa actgcaaata tgtcttacgc tttacctgat caattaccat gactgaaaac
     2281 gggtcgaaat attttccttc taaattacta accttgcctt taaaaacagt ttctttatcg
     2341 ccttgtgtca aatgctcaaa aacataaacc tttagacttg aagtgtcgac cccatttttc
     2401 ctcaagaaaa ttgcaatgtc tgattgcata aagttctttt ttggatctct aggccatggt
     2461 ctgggcagta aaattacgct tttcccgttt actatggcat taactaactc caattttctc
     2521 ccctcaatat ctccacttac gtggaaagta gctacatatg ccttatcaag tggaactttg
     2581 catctcgctg ccgcgatttg gattgaactg atacctggaa taatttcaac attgtcatct
     2641 ccaaaaattt ctagtagtcg atcaacaacc tccgattccg aaaagtttac gtcacctgta
     2701 aaggtcactg tggaataatc actatccgcc attttattat atacatcatt atataccatc
     2761 tcttggttct ccatagtgat ctcagaaatt tgttgtttgc ttctatctat tacactttct
     2821 attataatca gagtatattt atacccaata acatagcgag atttactaat tgcatcttta
     2881 gctgcatcag tcaaatatct tggggatcct ggacctactc ctataacaaa tagcttcttt
     2941 ggcaaattat gatatagatg atgagtaaga gagttttaaa cttggccttt ctttaattgc
     3001 atattcgctt tgtattatat taatcataat acaccataat aagacatgca gccgaacttt
     3061 aaccttatgt atacctttag actttttgtc ctcacttttt atcaatgtta gaagacactg
     3121 caataaatat aatataatat tacgataaac tcaagccggt ttatctcaca tcacgaaata
     3181 gatttaagat atttagctag aggaagcgaa gaaattaatc aaagtatact tttgtaataa
     3241 agaaatgaat gcagtcaaag aaggacccgc ctccatggca atatcccaac aagattatca
     3301 ttgagcagat gtttggctgt tgttcattaa ggtcgatgat cgcactatga ataacataag
     3361 atttattcaa acgtatacta cttatattat cttcaataag tccttcaatg aagtttctaa
     3421 tgcctcattc tgtaccgtcg tcatcatcct tagtaagtat aatttttatt gcagtcttaa
     3481 ccttggcttc tatatctata gctaacaaat ttccatatat catgcaacat gatcgtagtc
     3541 atgttgcaaa agccttagct ccaaaatatc cagaagttgg tctcccttct cctaaaggac
     3601 caacaatcct tgatcccaat ctaaaggctg aactggtttt cagaggatta agatatccaa
     3661 ccagtatggc ctttttaggt cctaatgata tcgtggtcac agagaaagat gcaggcacag
     3721 taagaagagt tgtaaatggt actgagctgc aacatgcttt acttaatgta agcgttgcaa
     3781 cgtatgctca cagggggatg ttaggtatcg ctgtcgctcc tcttactaca gcaacctcgt
     3841 ctactaacca tggaaatcgt aatactacca cctacgtttt cctatactat actcaagccc
     3901 agacacacac cggtgacgat gttacagaag gaaaacaacc attgggtaac cgcctataca
     3961 ggtatgaatt gatcaacaac aacaagctag taaatccaaa gatgctgtta gaccttccgg
     4021 caactccagg agctattggt gatggcggta aggtgattgt cggacctgat aataatgtgt
     4081 atgttactat aggcgatgtt ggcattaatg gtcatagtac taaagcacag aatatccaga
     4141 acgggagtga accggatgga actagtggga tactcagggt taatcaggat ggtcagccag
     4201 tagtacccgg cattttggga aataaatatc cgttagacct ttattattcc tacggaatgt
     4261 ggaatagttt tgggcttgct tttgatcctg taacaagaat tctatgggat actgagaatg
     4321 gtttgatatt tggggacgaa ataaatttgg ttgacccggg atttaacagc ggctacaata
     4381 agattgatgg catatggcta cgcggctata atatagatca agcagaaaaa catgttgcac
     4441 cccttcatcc aaatgattta gtcgattttg gtgggaaagg aaaatatcat ccgccccagt
     4501 ttacgtggtt tcggagtgta gctccgacgg gaattatatt ccttaattct aacaagatgg
     4561 gtaacctgta tgaaaatgat atgttcgttg ccgatgcaat aaatggaaat atttatcact
     4621 ttaaactaaa tttacaaaga acaggattac tgctacctag cggtccactt gcggatgggg
     4681 tagcaaacag ttctgatcct cttaaccaga taatatttgg aaaaggcttt ggtgggatta
     4741 cagatcttaa ggtaagtccc tatgatggct atctatatgt gctaacattt gacgaaacac
     4801 aagggacgct atatagagta gttcctgtta acaagtaaac aactaccgga taacgggtct
     4861 agatctgatt ttgtcttagt ctcttttttt cttctagccg taggtgaaac tcttatgccg
     4921 ttccgaagca gggacaaaac gtgccatcat gaaataaata tatttcacgt cttataccgc
     4981 ctacagccaa ttcaaattcc ctggcatgga ataattcgaa atcctgtagg ctgctacgct
     5041 agtttcgatc aaatagcaac tgttcaacat cgttaacgtt ggtttactgc aaagttattt
     5101 gcacttgcag caagccatct tcatatagtt tgtcatacgt acacatgtaa agtgtaccct
     5161 cccgggaggg cggcagcgtt aattttgtat ttttttacta aaagttttaa aaagtaatgt
     5221 ttcctatgag cgtatgaata aggcttttgg gccttagtga acattgttct aaaaactgta
     5281 gtttggccta ccaaaaagac cagggtagaa aataaagtgc cgtattaagc ttaatacgtt
     5341 ttgatccgat tatcgacaat atccactagt gagtaataat gttggaatag attttactga
     5401 attaggcccg ccatacggca ctaaacccgg gtcagccgcc ccaaaaagaa caggggtgtc
     5461 gcaaataaaa aaacaagatg atctgataac aataggcttt cgggtcaaac cgaaggaaaa
     5521 ggcaacattt aagactgtta atcgaatgga caaccagttc gctgtagatc ccaatacaaa
     5581 agaaccgaag aatcttggat tatccctcta gcgaacttgt gaccaaacta gtaacttttt
     5641 cataagagtc aattgattgc tgttcgcagg aacttttctt ttcagaaatc atcatttaat
     5701 gtaagtatag gtatccgaaa ttggcgggta gcatcaaaac caagtgaaaa attcaaattc
     5761 agcctctata cgctcaattt cggctcaatc gactgttaga ttcggcagta ctttcgtacc
     5821 cgctcatcag gggagaaact aagtctttgt caatctctac gccaatacag gcaacgccgt
     5881 caacaaccat caaattctat ggcctggaat attattgagt ttgacacact gacttaaagt
     5941 ctctagtcaa aggtgacaat acaatagggg attatttttt cattattttt tacacaaaat
     6001 cttaaaaaca aatgttacgt atgagcgtac gaattgggcc ttttcaaaaa ttcatcatta
     6061 atgtctgtat aatcacccat aatcaacaga gtaggcccaa aattctgaca actgaaccaa
     6121 accttcgaca ctattttact ggaaataatt gttacatatg acggctaatc cttcaatcat
     6181 tgtagcgact ataccacctc taatagggcc ttgtcatccg tttccattct ataggaggtc
     6241 cagcatggaa aattgaattc tttttcatac ttggaataat ctctattgct gcttttatgt
     6301 caacatgaac aggtcttgaa ctctgaattc tttctcgact ttttttatct tatttgcatc
     6361 ttcgattctg tgtctatttg ctcactcact ttatgctgct actagcagtt acattaggac
     6421 ttcctgttgg acttcccgtt tgaagcattt cttgttcctc caatgcatct tccatctcta
     6481 tggctaattt ccgaatagtt tctgcgttat atccttttgc aagttcttgg ttaacatgca
     6541 gtacagaagt gtttatcttt tcatatggac ttccaattct agctggcaat gcgtctgcct
     6601 gtgcttgtgt tactatgatg cttcctggtg gtacaaattt atcatcagga atactaacac
     6661 cgtcggttat tgtacttgat actcctattg caactctctt accgactttc gcagtaaaga
     6721 tcaagctgtt catacctaca aatgtgtcat ttccgacata tgcaggccca tgaacgatta
     6781 cgccatgagc taagctaact cgatcgccga catatatagc aaaaccattc ttaaacgcac
     6841 tatcatttgc cttcaaaaga gacccttgag ccgaatatcg tctgtcatca atattcttac
     6901 cgtgatctgt tgtttcaagt gcatgtaaaa tgacgccttc ctggagattg gaatagtctc
     6961 caatatgaat aggagtacct tcatcacctc tacatactgc aaatggtgca accaatacta
     7021 tctttcctat ctcgcagtcc cctataatta ctgcaagagg atcaacaaat gcagttttag
     7081 atattcttgg agatgtgaca tttggattga aatcagtttg aacgctagaa cgaatgtttg
     7141 tgggggtagt tgccgtagta gcgttgctag tattatttgt gatcttcaat ggttgttgct
     7201 gctgtaaaac tactgctgat gttttcagaa atatttggtt agaacctgct gccattataa
     7261 tggttatcag aacgaccgtc aatatcaaat tcttattaat ttttatatgc agacattttt
     7321 tgaaggggtt tatcattagt atatcgtcat tggttagcat ctatatgtaa acattt
//
LOCUS       DAOR01000221            3491 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35941, whole genome shotgun sequence.
ACCESSION   DAOR01000221 DAOR01000000
VERSION     DAOR01000221.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35941"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2772..2952
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1041 a          617 c          618 g         1034 t
ORIGIN      
        1 caatggttac ttatccatca tcatggttca tctgtatcaa attacatacc accaaatatt
       61 tcttcttaat atttgatttg cctatgacct ttacggaacc aatgattgct actttcgatc
      121 cctctgtggg taattctccc cttacataat cagtcgatat tggaccgaga gatataattt
      181 ctcaatatac ctacgtttta gaaaaacaaa ctgttattct cgttagctac cttgatggta
      241 gttgttgtcg ttgttcatgc tgttccttgt taacttcgtg accgcatatc ataatttgat
      301 taaaaaacaa atcatcgttg atggctttca tagggcagta gcattggaaa gtgagattaa
      361 aggtaggaaa agcaaaagca ttccaccaat tagaatatgg gaatgctatg gaaacttggt
      421 tcatactata ttcccttttg aattcagtca tctaatgaaa ccattgtact agtggtaagc
      481 acagtcaaag ggacaagaca agaggtaccc tcccgggagg gcggcaacca tattttttgt
      541 atatttttgt aaaaagtttt aaaaagtaat gcagcctatg agcgtgcgaa tgaggcctct
      601 tgtgtgttaa tttgactttt tcaaaaactg tagtttgacc tatcggaaag acccgggtag
      661 gaaaaaaatg acgtattaaa cttaatatcg taaggtagaa ttatcgatat tgtccattag
      721 tgtgtaataa tgttgacata gattttacgg aattaggacc accccatggc agcaaaccag
      781 gtaatgcgac acaagccagc ccaaaaggca ggtgttgaaa aacaagatga tctgataaca
      841 ataggctttc gggtcaaacc gaaggaaaag gcagtcattc aaggttatgt taatcgaacg
      901 tacaaccagt tcgtagtaga tcccaataca aaagaaccga agaatgttga attatccctc
      961 tagcgacctt gtgaccagac ttgtaatttt ttcatatgac tcaatttaat tgattcctgc
     1021 ttgaggtcct tttcttttca gaaatcatca tttaatgtga ggtataggtg tccgaaattg
     1081 gcaggtagta tcaaaaacca agtgaaattc gattcaaccc tcatatcctc ccgggaggaa
     1141 ggccatgaaa accaaccatc cattctcaaa ttccccgttc acgaatacta tcgagtccga
     1201 cacactgact taaagtctaa gtcgaaggtg acaatacaat agaggcctgg acgcttatac
     1261 gaataatcaa ttgtttggca aatcagaaat atgaactagt tacataaact cggagtttag
     1321 gtaactacga accaataatc aatctacaaa tagtagttta tttggtaccg caatttgttt
     1381 ctgtattgat ttcatgtcta cataatcggg gtttaggtaa ctatcaaaag ccccttcatt
     1441 tgttgggtct aatccttgct ttcgcatttc agaagcaata agttcaatca attcatctgg
     1501 attattatga tttagatgtc tttgctctgc aattgcatgt gttgtatgtt ccaatccact
     1561 tgtgcattgg gtccacactt tcaattttgc gaaaactatc aagaaacgac aggctatcct
     1621 ctctagtcat ggatttgaat gtcttttgat tgctaaaaaa caccgagaat ctgcttaata
     1681 aaattataat agatctccta taattatctg ataaattagt ctctgtcttc agtttgcgat
     1741 aactgcggca ttctctttgg agaatttcgc taccttttca tacagccagt taaagcaatc
     1801 gtgagctagg cactcggtcg cgttttgtat ctttctttca aataaaagtt cgctgtcatc
     1861 gtcatcgata atttgttttg tatttgtatt tgtgcctgca tttgtcttta ccttaacagt
     1921 ttgcatctga atcgatcaag ggatagtcat taaagtgtct atataactat gtattatatg
     1981 tatacccatc aacgccagat taaatatatc attcaaaaag gaatactcag gaatacaata
     2041 atacttgagt tgggggacta ccgattcaaa tatctaaatc atgagcaggg atttcatgga
     2101 taaacctcaa atgtaaccaa ttgagtgaat ggatacattg taaacagatt tgctgccaaa
     2161 atgctttttc gtttggaaat cataaatcgt ctcccatgat aatgcgtagt aagcctcaaa
     2221 tgcagtcggt tgcagttaac taaatatcgc tacgcctttt ggtaagttct atctgggtac
     2281 cgcaccatgg acaatacttg attggctcag gaaagtccaa ttaaccgcat tcatagttgt
     2341 aggcataaat ctccttgtcc tttgatatga gaaggcaata gtcttcttcc ttcatgtctt
     2401 ggcagcagat ctctattttc ttagatttgt caaccttgag accaaggctt atcattttag
     2461 ggaaaaaggc gccaatccta tccaggggga catcttgacc ctaaaatgtt tattcgtata
     2521 tcaattaaat taaaaaacaa tcagatccca atgggttact actagtagta gtggctacaa
     2581 tgcaattgaa tgggctttca agatggcatg gcattatgac aatctgcatc gtagttttgg
     2641 ttatacatag ctagaactat aaggttaaaa gtatttatat cctatgcacc cccatagcat
     2701 atgtcgtgaa tacctgtgtc cgggcatgtg aattcgagcc aaatcagtca cgacagcagg
     2761 atgatgttga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnccagagaa taagaagtta gaaatacttc gtctggctgg gatggctaca
     3001 ataattgtac ttataggatg gatgcatctt ttacaaccaa catggctagg aaatattgta
     3061 gtcgttattt ctgtattagc tggaggttat cccatattca aagaatcatt tttcgcatta
     3121 agaaaaggac gagtaaacat ggagctttca atggtaattg ctattgtcgc ttctcttgcc
     3181 ttatatcaat ttcttccagc tatagtaatt accttctttg cactcttgtc agaattcatt
     3241 gaaggatata tcgtagaaaa agggagaaaa aacatacaac tactctataa tctatctcct
     3301 agaaaagcaa ttattaaaag aaacaacaaa aacggagatg aggaaagtgt aacagttaca
     3361 acactagaaa ccccaatcga cgaagtaaga cttggtgata ttgttattgt aagagaagga
     3421 gatactattc ctgtagacgg tcatattctt agaggctgtc tcttatacac atctccgagc
     3481 ccacgagact a
//
LOCUS       DAOR01000222            8402 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35942, whole genome shotgun sequence.
ACCESSION   DAOR01000222 DAOR01000000
VERSION     DAOR01000222.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35942"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2206..2518
                     /estimated_length=313
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2756..2987
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6206..6480
                     /estimated_length=275
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6795..7023
                     /estimated_length=229
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7464..7644
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7973..8228
                     /estimated_length=256
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1979 a         1398 c         1268 g         2271 t
ORIGIN      
        1 agtctgattt gcttccatgt tactttttct gcttttcagg cttttctgca caaactttaa
       61 tattctcgtt cgtaaattgt aatacttttc aaatcactta gtagtagata cacaaattca
      121 actatcgtat gcctgcaaat ctgcttgccg cggctacccg aactgattct tacaatttac
      181 gtatttgatg cttggtgtat caatcttcag agccaaccca ataagccttt taataatatg
      241 gaagaggtta tagcacgatt aataataata tgcaaaattt ataccgcaag taccgagaat
      301 atatcctctt taataagagt atatttattg ctggaatctg tgcatttctc gcggctgctt
      361 atgttactca agcctcttat ttgacatata acaaaagtaa cataacaaat tcaatagttg
      421 gtttgactac agaatatggt gtttatttac caatttttgc actcttattc tatctggaca
      481 atcggtatcg gtatgtagat cctctgacgg gaactagaaa tcataaggtg ataatcctgg
      541 acatcaaaaa acttattgct tccttttctg tttccgaaat tgtatattct gttaccaaaa
      601 tatcttctaa ttatcagcta ctccagcttg gaatacaacc gtatcaagca tcaatggtta
      661 gctcactaat agcatctctg gttttttacg tggtaatcaa cgtcattgtg ataaaggttg
      721 taaagctgtt caatagatga caatgagttg gaatgaaaca acccttcatc atagaccatt
      781 cacatcgttc atggaacgaa tttatactct gctacttata acatgtaccg caaccgtcgc
      841 ggatatacgc cgttttcctg gatctagaaa ataccctcat tttaaaagga aataagataa
      901 aatccgtttc cgccgacaat actgataggt acaactaggt ggaagacgat aggagattga
      961 ctccggtgac accattatca gtgggctggc ccaacagctt ccaaatgtat gcagattaca
     1021 tgagaacaca agcatctgaa gaatgtatca acgagatatc gttacttgcg aagcataata
     1081 ctatcgccat cttgtgtgca gaagcgctgc catggcttgt catcgcagat ttataagcga
     1141 ttctcctacg atgctcggtc ttgaagttta tgatatcaag aacaataagc atcctgacct
     1201 tcccatattt actttatatg cccaaattct tagtttcgaa ataagatatt ctgcatctaa
     1261 cttgcaagag gagcatataa agaatctgca aaaaaagacg agccgaatcg aaactataat
     1321 acaagaccga accaaagcaa acaaagtcct gaagcgctga cgtaatttag ctactttgtt
     1381 ctcgcgatta gtaggttagg actggttccg cttccaagat gtatccccac ccagctctga
     1441 aaaagtgaaa ataatacgta agaatacata aaggttaaga gggatagaag atatccttgc
     1501 aaaagatttt ggtacgtgca ccatcggagc tggctcataa actgacggaa acccttaggc
     1561 accgctacga cgttcgaaca gaaatacacg aagatgacag taaagccatc tgtgagatag
     1621 aagcaaggat aacgaggaac tggattacga tttgtaggtt tgcacctgac gagaatttaa
     1681 aagatatact tacgatgttt aaggtgaacc ttgaaataaa aggcaggaga taaagttgta
     1741 ttttttcatt ctaaatcgtt caccgcattt ttcgaatgta catgggtttt cgaattccta
     1801 aagaaaacgc gcacctcttt cttgtggatc ttcgactagg ttattgctat cctattgaat
     1861 tgttgaaaat tctcgaactt gttagcttca agcataattt cctatgggaa ctttaatcgt
     1921 acgatttttt acaatttatt tatccgaata cattcaatat cagattaatc acaagtcttc
     1981 aacaagggtt ttttcataac taaaaacaat aattagtcaa gctaaataat aacaatttct
     2041 aaggtatata aaaaaaagga tatcgcaaaa gccactcctt attattgtgc tcaagtatca
     2101 aattgacatt tcgtcttgcc ggattgaaat ttaatgcaga aatattcatg tgataaaacc
     2161 cagcaacagg atatcgaagt attccccgcc agggaatttg agaacnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     2521 attgacgtac agattgacaa agactgttag tttctcccct gacgagcggg catttacaaa
     2581 aatgctgccg aatctaacag tcgattgagc ggaaattgag ctattaagag cgtatgaggg
     2641 ttgaatatga attttcactt ggtttttgat gttacctagc aatatctgat gcctattgta
     2701 gcattaaaga tgatttctgg aaagaaaagt tcctgcgagc aggaaccaat taaatnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc aggaaccaat
     3001 taaattgagt catatacgaa aaagtgcaag cttaatcaca agtccaattg atggattatc
     3061 tagcatccgt ttgggctgct ttgttgtggg atctacaatg aactgaccat acatcctgtt
     3121 tacatagtcc tgtatgattg ccttttctct tggtttgacc ctaaacccta tggttattgg
     3181 atcatcttgt ttttctacac cctgcccctt agaggggact tgtgctggtt tgttgccatg
     3241 gggcgggtcc taattcggta aaatctatac caaacgttgt tacccactaa gggataaatt
     3301 cgataattcg accttacgat attaagttca atacggcatt ttttgtctac ccacatcctc
     3361 ccgggagggt aaattacaac tttctgaaca ttcttctata acacccaaga gtgggcattc
     3421 gcacgctcat aggaatcatt tctttttaaa agtttatgta aaaatgtacg gaaatagggg
     3481 ttgccgccct cccgggaggg tactgttacc gtaagcaatc taatgtacaa gaacaaacta
     3541 tatgaagttg gtgcctgcaa ggcaatgaac ttcgccctat ttttacgtgt atttgctgat
     3601 gtatgtcata ggctctttgt ctagcaggcg acgtcatgac ctagatatgt tgatatttta
     3661 tgccggtgcc ttcggatacg tagaatagct ggaaattgtg gaaaacggtt gtaactcgag
     3721 aaagtttaga ggcaaataac attgctggtc gaatagtgat atagtatctt cctccacaga
     3781 atggaatttg cttaaccaaa tcatttttta ccgctacgta tttgccttta ttcaattata
     3841 gatgacttcg taagatataa ttaggatttc gggagagctc ttgtattctt tattgatctc
     3901 atctgtcgtt tgtacattga gattaattcc tctacttcag tagcgtcctc cggatttttg
     3961 tcgactctta tcttgccagt aaatttcggg aacctgagtg caaggccata attttccctt
     4021 attgaattga tgcctgcagt atgtgaagga ctaagagtta tttcagacgc actaacctcg
     4081 attactattt ttggctcaaa ccatagatcc atttgcattc cggtgtccac tctaggatgt
     4141 atgtgagtga ttcgggtgtc ttctagattt ttgtaaaaat gctcgagatg caggtcagta
     4201 aaacctgtcc ctactttaca agtacttcta aacatatccg ccttagaatc atatgccgcc
     4261 aacaaaaggg caccatattt tccaactcta cgtcctctgc catagagtgc accaactata
     4321 accaaatcca gggtgtcagc aaggtcgctt cgatactcac gttttaactt aatccaggca
     4381 aaccctcttg cccctgctct gtacgagctt gtgagctgct ttatcataag gccttcacag
     4441 ccatgctcta tggcaacagc cataagtttc tcaagatcct ctgttttttt tacaattgct
     4501 tgggggacca gtctaacttt ttcgttcttt aatccttttg tggctttttc aattagttta
     4561 cgtcgttgga ggtacggtag agaagtcttg tcctctccat cgatatataa tacgtcaaaa
     4621 atatttatga ctacaggata ctcttctatt gcttctttga ttccatattt tcgtctgcga
     4681 tgcatcagtt cctgaaaagg aaggaattcg tctgtttgag cattaattgc tacgacctca
     4741 gcttctaata tcgtttcatt ggtcttgatt aatttttgga ttgcttcagc aacatcggga
     4801 tagtggtgag tgattttttc taaccttctt gaaaatagct ccaccttctc ttttccttta
     4861 tggacttgaa ttctttctcc atctagtttg tactctgctg ctagagactg gcccttcatt
     4921 cttaccatcg cttcctcggc actccttacc cgttctgcca gcattggtct gactggtttg
     4981 tataatgtaa tttcgagtaa tctcagggat tcgagacctt ttatagctag tagtttggct
     5041 actgttccga ggtcacttga aacattatag gcattttcta gtatttgtct ataggacttg
     5101 tcacctgtaa atgctaatgc taatgcatcg attaccgtat aatcggcaat acctagccgt
     5161 aaggttccca ttatgaattt cattatgtat cggctctcac gcggagtcgc atcactcaaa
     5221 aggctactta caagacggag tttggcatcc tgtgaacccg tgccaccaga atgtgctatc
     5281 ttatctaaag tcaaatatac tctttcaaca gtcattttct cagcaagcaa ggttgtttgg
     5341 ttttttgatt tcattacttc cccagccgta tcgccaacgt ctcccgtttt gcgataaatt
     5401 tgttctatag actcaacatc taatcctgta caaagagaaa ttgcacggat agccattttt
     5461 tctgccaagc caagttcgac accttcatag tcagggcgga gcttgccctg gataaggtat
     5521 attacacgat caataatgtc ggcgggcgtt tttctcaaaa gagaaacaag gtaatctgta
     5581 agagcaagtc tactggaagt tcgctccatc tgctcaaatg tatctacaat tatcgaaaaa
     5641 tccaatactc tttaattaca gcaagacacc cataggtttt aagttattca ttcaaagcat
     5701 ttctcgttag gcagttcatc agtagctagt gtctacccaa caatgtcgct cgaattgagt
     5761 ctttgtcatt tgaccgtcta gagtttaact ttttttgatt tgtctttctc aagtaatcat
     5821 caactcgaga ttgttgccgg tactacccag acagtcacat caccactcgc atctaagtga
     5881 ccattcttgt tattcttatt ctttagtatc acgctaactg ttaaactatt actaatatca
     5941 tctcctttca aagttgtgct gcctgcaacg gtttgatcat tcttaccaac cgctacaact
     6001 gaaatatact taggaatatt tccattatcg ttatgcgtac gttctagtgt cacctttaaa
     6061 aggtgtggat tacttctatc aacatctgtt atcttgacgt cctttatgaa gctcttgtcc
     6121 tgaacatcta atccattatg attgttattg taggtgtact tgtagttgtt attgttattg
     6181 taggtgtact tgtagttgtt attgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 tattattatt attattattc ctgctttcct tattgctgag ctgactcacg atcttgccta
     6541 tatgtttgaa ccctacaaag gatactgcat cagtaacggg tgaattactg ttcccaccgt
     6601 aatgtaattt gagggcaggt gtagaacctg tatgataatt tacggataat ccatgaaatg
     6661 gtgataacga tgtatctgtc ggaggagatc ctagaaatgc agacgcataa gctgtattat
     6721 gatttgcagg gaaaccacga aatggcacga gtgcagatgc tgaaggtgcg cccaacataa
     6781 ttattgctaa cattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnatagtgg tatcgttgtt actaaaactc caaaacgttt accattacaa tcttgcattt
     7081 gaaagttatt cggcaaagtt gtttaaacac tttgtttatc ataattaggg aattaattta
     7141 cagaaaaagc gtctaccaga taatatagtg aacgtagcta tacaatgcta ggtgtttcta
     7201 tttatctaac tacctttaag acaacctcga attccaatat gaaggtcagg gctgtagaac
     7261 aacaattatc ggattatgcc taacatcctg ccaatggcga ggttaacgcc gcctgcattt
     7321 gaggttatag aaatgaaccc ttttcctgcg ttctaggcca tgtgggagtg ccaacaatta
     7381 atccttgtac tctatcatga atacgtagaa aattcatacc ttttactgta gtcttgaaag
     7441 ttctttctcc atccagatat tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnntctttc tccatccaga tattcgatta gacctttttc
     7681 tagtagaagt cgcacatatt ccttggttag ggtatgagaa aggtatacat cgtacattat
     7741 ttttgttaat cgtactctat tgccattggc actttctaat atttgcgcga taatttcgtt
     7801 tctatctctg tacttcatat atacattgtt ccacgtaacc attacataaa aatgtcgcta
     7861 tcataaatcg acgattaata aaaatacttt tattgacttg gattagagtc gtccttcctg
     7921 cctagctgag ccaacgctta ttttcctttg tatacttaga gttaattctt cgnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnngt cagaaaggga ttctatattt aaaatatctt gctttgaagc tatctcttct
     8281 aggcatttcc ttctggtatc acctccatcc agagggcgtt gaatccttcc gagttctcgt
     8341 tgagcttcta attttgcctt ttcatattca gactttgcac agcccaagga tctgaataaa
     8401 tc
//
LOCUS       DAOR01000223           13848 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_22837, whole genome shotgun sequence.
ACCESSION   DAOR01000223 DAOR01000000
VERSION     DAOR01000223.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_22837"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    12552..12800
                     /estimated_length=249
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4149 a         2801 c         2787 g         3862 t
ORIGIN      
        1 taacaccttt gctgatatta ccgcctaaag tggaggcgga tggtggtggg gcggaataaa
       61 gagaagtaga agaggattgg ttatgaaagc cacttaaatt tgatgcacca ataccactat
      121 tacctgatat tccaccgtta cttaataatg gaacatgtga ggatgatttg ctaagggcca
      181 tatttggaaa agaactacta ccgatgatta gcatgattgc aattgaaata acgacaatgc
      241 caaataaagt ccgaaaattt gcatttctct caagatttga caagatactc tacactatga
      301 gcactatgag aacagatgta ataaccatta ctaggaagag acttacaatt aatttcattt
      361 taactcgata tatttgcatt tccgaatggt tctccaattt tattacggtt atatctctca
      421 gagttctgcg aataaatgag tatgttaatt aaaaaaatga agtactggta gcagtacagc
      481 tgtctaaata tcatactatt atcagatgat atgtcttcta cttcctttag tgttcattag
      541 ataactgtac ctaaaccaat gagtcgagtc tacatactaa tcctacggcc tatagcgaag
      601 gcagcgacaa ccaaattgcc tataaaaccg taacaagagt tgatctacca tacaagtaat
      661 aggtaagata taaaatatca attactgatt tgaaaaaaca aattgtaatt aagctttcga
      721 gatttgcttc cataaagata atacctagca aagactttct tctagttctc aggctcaaac
      781 ctggtagcac tcctaatatc gcaaatccac tgacctattc caatgtcact agctagtcca
      841 agattaccaa atggtccggc attgctgggt agcgaattta tatcaatagc attgcatatt
      901 tttgcttgta atgacaaatt taaatggaaa atgggcagtt ttggccattg ttgctatgac
      961 cttggtccta ttgacagccg caaccttcgc tggatcggga ccagttttgg ctgttaagaa
     1021 aagtagtacg acactcactt cggctcatca tattaaagga gtaaagattc ttcatgtcca
     1081 tacatacaag ccttcagttg taaccgttgg cagtacattc agtttacggg caatagtgta
     1141 taacaactct tcatctacaa ttacgtttgc caatgggaca tgcacttcac aactgtctgt
     1201 aagatttaat aaaaatgtca tgatagaacc tcagcctgca gctgcctcct gtaaagcact
     1261 accgcgatca gttgctctaa agcctggaga acagttgctg atatcaagtc caaatctttc
     1321 tggtatagcc tacagagcca ccgctccagg aatgaccaac gcaacaatga ctttcaaata
     1381 tggagttgaa gccattactg gtaaatcccc tattggcgat agtatttcga aagtgctaac
     1441 attcaatatc cggggttcag gtccacaacc cgctggtctg caccctagcc cgcaaccgag
     1501 tccgctaaag ctccccgtac catagtctgg gagaaaatct catttttatt tcatcattta
     1561 ttacgaatat attgtggcgc tgtatgttac agttgcttag tggacagcag actattatgg
     1621 ttaacgactg tttgtgacgc tcgcttcgca aatttttaat gatgcgttaa taagcctcaa
     1681 ataggaatta atggctgccc gcaatgcaac aatatctgga gtctttagtt ctaataatat
     1741 actggaatta gtattctcat cttttgtaag ggtaattttc gtctcggaac cggacgccgc
     1801 agcgtcacga ttacacattt ttgtgtctgc gcctatcgca gcataaatag atttagcgag
     1861 aagggcagat tcagggagca ttattgattt aaaggaaatt tctatttggg ctttgcaatt
     1921 tgtcggtagc gacatcatag gcccccgctg caactttgta gctgcatttt ctgcagtacc
     1981 aaacacctaa cgcaattcga caaaatttat tagaaccgca tgaggggcat cgtctttttt
     2041 gcttcaacgt tcgatatgcc ttactgtacc tcttgcgtac agtcgctcca tattttacac
     2101 caagcccttt taataccgtc tgtccttgct ttcttctact taccagcctt ggtcgactcc
     2161 cttagtttgg ctcttatctc ctcaccttta atccttgcag tcgccgccgc tctctttagc
     2221 tgctctactg caaaggctcc tgttgatcct ttctgtacag cagcaaaatt ctgatcggaa
     2281 ttcgtagtta tggtgattct tgcatccata catgcctcct cttctgcggt tggatctaaa
     2341 atcactgatt cgcctattct aactgctgta attgaaacgg gtaccgtggt aagtggtgga
     2401 ttttgttttt ctccggtgtc aacggctttc ctatccctta tttcaaagac aggaagttta
     2461 ctattcatca atgccgaaac tgctgcatat gatgtggcat ctaacagatt cccatcagca
     2521 ttgataacac tacagtcgac aaatattaca tagacgatct ctccaggtat taaagctaat
     2581 ttacttaaat cgagcatttc agattctctt atgccgcggt ctactactct tgctagttcc
     2641 accgtttctt cgtcaggtgg gccaggttcg atatagggcg aagctgttgg caacacctct
     2701 gcagacagaa ttagtgcccc cttattttct agcccctcga atggttcacc ggtctcgacc
     2761 tttactcctg ctaccacttc ggaattacca agtttgaccc tcgcggatcc attcgctttc
     2821 tttattaggc ctacttcaat ttcgatcggt cttatctcat ccagggttct tccatcgagt
     2881 ctttttcctt ttgagataga gtcccacatt tgctgtttgc gcaagtgctc tactattatc
     2941 gttgaacgtc ttgatgagct cattgttcct ctttcaactc tgtttcgttg ccgaaatact
     3001 tttgcatcaa cgcttttctt tgaacttcat aaaccagctt gcaacccttt acggcttcgt
     3061 cgagacattg attgaattgg tcttcattta gaacgccatc aagctgtaag agggtaactt
     3121 gttcgaggtg ctgcatatag gccaccggca tatcagcatc tccctcctta tcctcagtat
     3181 cgttaatatc taagactatt ctgtcgtcga ctttacccgc tgcacacgca gacaccatat
     3241 ctcgcatgtt aatcccagca tcggctaagg ctacagatgc cgctgtaatg ccagcacatc
     3301 tagaccctcc atctgcttgc aacacctcaa cgtagacatc gattgcagcg cgtggataat
     3361 cctccaacat caaggaaggt tccaatgctt ctctcatcac ttttgagatt tcaacttccc
     3421 tcctagatgg agcagggttc ttgcgggtat cagtagaaaa tggcgtcata tgatatctgc
     3481 atcttaaaac acatctatca gatagggcca tatgttttgg atgtacttcc cttggaccat
     3541 aaacacctac tacaattttg ttcatgccaa attctatgaa cgcggaaccg tccgcatttt
     3601 ttatcacccc aactgtgatt tttatttccc tcaattgatc tccggtacga ccatcagttc
     3661 gcttaccgtt ttcgtcgatt aaacttctag tttgagtcat ggaacttttt cgcccttttc
     3721 cacttctagc tcttcctcga cttcctgaag agtaggctgc aattcctcat tctgaggagc
     3781 attttgtggc tgagctaatt gagaggtgtc tgccggtgcc gaatcggata cattaagcag
     3841 agttttcacc ctctgtgtca gattcggtgt gtgcgcttct tcctgtacca tcctgattgc
     3901 cctaaccgcc aattttattc cctctgaatt tctgccagtt acaactacga tcccattttg
     3961 accaattaaa actctagtgt gcgtagcctg ttcaatcgtc tgtatcatag acccgcgttt
     4021 tccaataagt ctcggaacgc gggtagcaga aattttaata aactctccac taggaacctt
     4081 gcctaaatct ttatcctgaa tcgttaccat aggatccctc gttctatcaa aggcaataat
     4141 cctcgcggta attaaatcac ctatagccaa ttctcttccc atatcatccc tggccggaga
     4201 aaagtctctc ccaaatacgt cttgcgcagg caggtgagca gagaagcatg agttgatgtc
     4261 aacatcccat gacaaggaag aatgatgcat tatcttgcct attacaatat catttactcg
     4321 gggaatgtat accccagaca atggtattac tttgacacct tctctccctg cttccgcaat
     4381 cccgatcctc gtggagatga tagaattgcc tgccttaatt acgttaaata aaggccggaa
     4441 attcccttca atgattcggt caccaggaac gacgtacttc cttctaattt cctgcatgct
     4501 ttaacaattc accctttcaa ttgggcccct atcagttaaa agctctattt tcattttttg
     4561 tctctatcct tgggtgaata agacagactt atattttaag tgtcgaagcc catctcctac
     4621 ccactttact cttccattac ttgagctgtc ccttttgata cagatcctat cctatccagt
     4681 aaagaactcc tcattccggc atttatttct aatataaccc gtaaagagcc gtctgcaagc
     4741 cactgctcat ccttaaagtt agatgtagtt ttcaataaat tataagattg tgccgagaat
     4801 tgagcgggaa tagttacagt cagtaataag ttctctgact tcagcggcaa aatccttcta
     4861 agagcatcaa ctacgttttt tgcttggtcc tcagccggct tgaaggggtc gactgtaagt
     4921 ctcacctcgc tcattgcact ttcgattctc ataaccggat gcggtatatg cgtcttagga
     4981 tctacaaaac ttttgtttat atactgaata atttgtctct tcttctcctc aaccattttt
     5041 cgacgctggt ctgttgtgag actgaggtca cctttggaga ttatttgcct ggcaacctcc
     5101 atcggatcag ttgacttaaa gtactttgct aacttttcac tcgccgctct agaaccttta
     5161 ttggcatcag aatatatttc atcagaaata agtacacctg ataggtccgt tcttttgcca
     5221 agtttatact ctagagcagg atcgggtttg acaagaatct caaatttatt cccgtctaac
     5281 gttaaacgta cagtagtaat tttattctcg accatttgcc ttataaaact gttcttcctg
     5341 aaatatatat ctgcatctca gtgattcagt gtctggatct gtgaacctta cttcaggtcg
     5401 gcgactcgtg aaggtctgca gtcccgggaa attcgaaata aagatcgcat ccggcacacc
     5461 tcctaacgct gttctcactg ttcacctgac ttcgaactag aattatactt gtgactttag
     5521 agaatttcac aatcacatca ttgcatttag tttacaatag cctcatcgtt agcttttcca
     5581 gacggaggct aactaacaaa aatataggaa ttatctccaa tgcgtaaggc ttgtccaggc
     5641 tacaaataga ggtgccgtta tggcacgtgg gcatttagta aggcttcaga aaatttactg
     5701 ccttcggtag atttttaagg acgccttgat caaagtctga aatagagatt gacttccatt
     5761 tctgccgaag ttcttgagag aactgatgag aggattgtga ttaaatttaa caatatttct
     5821 agacaatata taaacgctat tagaagaatg gccatatgcg aggttcctac tttggccatt
     5881 gacgatgttg taatcctcga gaattcatct gtaatgcacg atgaggctgt ggctcacaga
     5941 ctaggactta tccccttgcg aacaggattg gatcgattcg tgatgcctca agattgcgat
     6001 tgtaaaagta ctttagggtg ttcaaagtgt agagtgttac tcgtattgga ctctgaagca
     6061 atggaaaaga cgaaaatcgt cacttccggt gaacttttat ctgaagatga acttgtgaag
     6121 ccagttagca aagatatccc gatagttgtt ctcgcaccgg ctcagaagct caaattcgaa
     6181 gcatacgcaa ggttaggggt ggggaaggat catgcaaaat ggcagccaac ttcagcggct
     6241 attgtcaaag atggaaagga tgacagcgag tcaatactta caattgaaac gaacggagcg
     6301 ttgacagcag aggaaacagt tgatgcagct ctggaaaagt tgaacacgaa attaaagagc
     6361 tttaatgaag tcgtgcgctc tcttgagcca aaggtagcta ggtagattta tttggggatt
     6421 cctgataacc gacggaatag tcataatgtt agctaactct ttagtcggta acatagtatg
     6481 gactttacgt aaagctttta agaagaataa aacctcgatt tggaggtccc tcgaacacga
     6541 gatttctaaa gctagatcga ttagaagaga ggtcaatatc tccagattgt catctgtcac
     6601 caaaaatgac gaagtcattg ttgtgcctgg caaggttcta ggatctggag aaatggatca
     6661 caagttgaca gtttgtgcct tttctatttc agaaatggct gcaaagaaga tcaccgacgt
     6721 cggcgggaaa gttataaccc ttaacgaatt gatagacaga tatccggacg gcagaggagt
     6781 gcgaataatt ggctgaacaa catggattaa aaaaatctta ccccaaatca gagcccctgt
     6841 caacgaaaac tgaacaacgc gcgctagtag ttaacggggc tgggtgtatt gcaggtagaa
     6901 tgtgctctca tgtttctaaa ttgcttctac aaggaaatag agtcacgatt gtaaatgcag
     6961 agaaagctat gctatcagga catagatata agacaatcga actctacaaa gaacatcttg
     7021 aaatcaattc tgcaacaaat ccaatacatg gaccattcca tcctcgaaga ccagatagaa
     7081 tactgactaa aatggtaagg ggaatggtgc caaaacggaa ggccagtggt atcgaagcct
     7141 ttaagcgctt aagagtttat atcggcattc ccgatcagct taaagactct aaaatggaat
     7201 tcttcgaaga ctcaaaaatt acgaagccag cctcatacta cattacaata ggcgatgtag
     7261 cgaaggagat tggatggaag ggagagatgc ttggtagtta gtaaaacaga cctttatcca
     7321 ggccaacgaa agacgtgtcg agctgtggct acaatatcaa agggcaatgg cagagtgaga
     7381 atcaataata tcccagccga actgatagaa ccacagattg ctaaggaatt ggtattaact
     7441 cctctcaatc ttttaggcga gctaagaaat aaagttgata tcagtgtcaa agtccaaggt
     7501 ggaggattca tgggacaagc gttcgcctct gccgttgcaa tttccagagc gctaacgggc
     7561 gagggaaaag ggggtaggga cccaaaagac catccattga caaagagcgt acgtgaagaa
     7621 attcgaaaaa aaattgctga atacgataga catctgctgg caggtgatcc cagacagagc
     7681 gagtcgaaaa aattcggagg accaggtgct cgacgcagga agcagaaatc gtacagatag
     7741 acgcataata gtatgaaaaa ggcacttgct ctgacatcgt caagatctcc atccttattg
     7801 ttttgacgat ttcagatttg ttgccgatat cgataatttt ctaataaatg gcatgatatc
     7861 agatatagga caagtaattt atatagagta aaatacaccg taattaatac aagtgccgtc
     7921 ggattcacat gcagagacac tgaaggcaat ttctgatgac aaatctctgc ttgtgttcaa
     7981 gactattgca ctgaccagcc ccgatagcag ttcgctacaa cacaaaacca aattgacacg
     8041 taaacaatac tatacacgga cctataggct aataagagct ggtctaatta agagaagaaa
     8101 cgggaagtac tttcttaccg cgtacggaaa aattatttat gaagctcaaa agataattga
     8161 aaatgcgcat acgaattttt ggaaacttaa agccattgat tcgctggaag tatctgacga
     8221 tgagcgctct ctgaaagagc gtaggaaaat tatagacact ctgatcgatg acaaagatat
     8281 caaacaatcc ctccttgcaa aatctatgcg gtaacactat aagatacgat cgtgtcaaat
     8341 aatcagcaga tttagacgtg tatatcccag accacaaaaa caaatgcaaa agacataatt
     8401 gcccccaggc acgagcaatt aagcaatcta ttggaaattc agcagagtcc atgaacattt
     8461 aaccaacttt ctatgaacct ataaagaatt caccgctttg taatgtccct acgatcaatg
     8521 gcaatatagt gatcggtcaa ttttttctca taacattttc cacatagatc tcctacaatg
     8581 ttccattctg gcattgcttt gtatctaaag cttatgtgat tcccacaaac aacgcacttt
     8641 cgatcttgaa acttggccat ttgatgtaat atcttttcac attacttgtc taaattaaaa
     8701 gttgtggcgt gatggtgtga ttggcctgcc tgtccatgct caaaacttag agcttggtta
     8761 gaatagcaaa ggccaattaa aagaaattat gtaaaccaaa aaggtttgat taatctgctt
     8821 ttgtttaagc ccagtaacat tgaattaagc attgtgacta tataaagagg acattacacc
     8881 tggcaggtgc aacgtttaca ttcaactata tgaaggggct ttagctcaaa atacaccggc
     8941 tgtggcgtcc atagaaaagg aatttgaact gacgcctatt aagcgactcc caattagata
     9001 ggtatgagtg acgaacataa aaaaaaattg aacttattcg ccgcgattta gaatttaaaa
     9061 actcgccaaa tatgccagac aagcggaaca tacaactcgc ggcaaaagca ttagagacaa
     9121 caaagttcaa agagactgct ttcggtgaat acgcaaccgg gaatagacgt aacacagttg
     9181 gtgttttaat gtctgtatta ggctggaatg gtggaaccca agcattatca gaaaacgcgg
     9241 ctgaagcatt aagaatcaga aaacaaagca gtgaaaaaga caccgaaaac aggctgattt
     9301 accgccagaa agtatgcgaa tttctgtcag agcacattgt tggacgtatg gactacttgt
     9361 ctcgtgacaa tagctttaaa gaaatagccc aatggcttag attatttctc gttgattaac
     9421 caatagtctg aattgcttta acggcagttt acgcagttca caacaactac cttcttgtgg
     9481 acaaagtaaa ttctttagag tctatgaact gctctcatca caaattatga cgtcaaaagg
     9541 ctgtggattc accaagtcct gtctcaattt tgctatgtgg accaccagaa tgcggtaaga
     9601 gtaaatatct aaaacaagtt agaaactact tcgaggaaaa atcggtattc attgatggaa
     9661 gctatggcag taaagcaggg atattccaaa tactgtatga caaaagactt gagtacgagt
     9721 ccgatggcag ggcgccaatg acaattcgaa attagtttgg aaataataat aataataaaa
     9781 ctaacaggtc caaaaacatc taggagtgcg tcaggaccat ttgaaactat ggaaggcacg
     9841 gttcatagtg ttgaaccgat taaaggcgtc cagagaatcc atatcggtcc aatagcggca
     9901 cttgcgccag caaaaacgat agacgacagc aatacacaaa attatgctct acttggaaga
     9961 ggaaataaaa gtaacttcta cacagctaac atgatgtcga aagtaaaaac tgtgcatgat
    10021 gggactgaag cagaagaaga tatataatat cgattaagat gggtttatta gcaaagaggg
    10081 acatcgtgaa ataattatgt gggttggaag aaccgctatg atagcacttt ttattctagg
    10141 agcaattaca gctgtggtgg tttataccat ctttcacgac gttgcaccgg ttccaggtgt
    10201 aatgtcctct ttatatagga aaaatataac tcctcaagat atagctgcag cgaatccccc
    10261 ggctgccggc tctggcagta caggtgctag cactggtcct gtgggagcct ctgtgactat
    10321 tctggcaggt gcatttacac aaggaaaccc agcttatgct cctgacaccg ttgcggtaaa
    10381 gaagggagat gcagtggggg taaccaacaa agattctgtt ccacatacaa tcactaatgg
    10441 taaagacgca agtgatccta cgtctggaaa actttttgat acaagcatta tcagcccagc
    10501 cgcatcagct caatttgcaa cgaaaaagtt ggctgcaggc aattttccct tccattgttc
    10561 catacatcca tacatgacag gaacgctgaa agtacaatag agaaaatacc cgattcagtg
    10621 tttttattct agcatatggc ttttacatca gggagcaatt gtctgtgtac atgatggatc
    10681 ttactcctga aggaagtggt gccagaggaa attgactgca acgccacgcc attaataatc
    10741 cgatcaattt ttacaggtga atatccacat tcctataaaa atcataaact ataaggacta
    10801 aaattttaga ataactccca ctagacggct aagcgcattt atgattgtta agaagttttg
    10861 cagattttct aataagaaac gtaacgtcgc caatgcacct gggacatttg ttaggacaag
    10921 caaatgtcct tatattatat acaccttaag gtaaagatat tacaatgtcg ttcttagaag
    10981 caaaaaattt gcacgctagc atagagggca aagaaattct gactggtctc aaccttgtaa
    11041 taaacaaggg tgaagttcac gcccttatgg gcccaaatgg ttccgggaag agtacgtttg
    11101 caaacgtatt gctcggacac ccaaaatata gtgttacctc aggcgacata cttgtcaaag
    11161 gtcaaagcct tctgaatcta tcaacagacg aaagagcaag gaaaggcatc tttcttggct
    11221 tccaatatcc gatcgaaatt gctggcgtcg gctatagcca cttcttaagg aatgcttaca
    11281 atactttaac caaaacatta ggagaagaac agaagaatag agaggttttt ttgactgtaa
    11341 gagaattcca cgaatatgta aagagaaatt tggattccgt tggtttagat ccagcgtttc
    11401 ttagtagata tctcaacgaa ggattttctg ggggagagaa aaaaaggtct gaggtaatgc
    11461 agatgctagt ccttaagcca aatgttgcaa ttcttgacga gccggattcg ggcctagata
    11521 ttgacgctgt gaaatctgta gcagaggcaa tcaacaattt aatagatact ggggcgggag
    11581 ttgttgtaat tacgcattat gcacggatcc tcagatactt gtcaagatta gatcatgtta
    11641 acgttatggc aaaaggcaga atcattaaat caggggataa aggactttca gaagaactcg
    11701 aaaccaaagg atatggatgg cttggcttag aagaaaacta ggcttccagt atggattata
    11761 ctttcctata gtaaaattac ccggatctag ttttaaatat agtttataca ttttattatc
    11821 aaatggcctc acgaggggaa atccaggacg gagcacgatc gccatcatcc tcatcttcgt
    11881 cagtaacacc aatttatcta aatttttcat tctttaaggt ggatccaaag tggagatggc
    11941 ttaacgatat agcaaaaggc gaggccgcca aggagttttc aatcttaata gaggttgcta
    12001 atacaaagat gagagttcga acatattcca cgttggggct tcgaccagac acggacttta
    12061 tgttatggat gatttctgat tcagttgaaa agatgcagat tttgacatca aaggtttatt
    12121 ctaccctgct tggaaaatac atcgaacctt cacacgtata cctttcttgt cttcgacagt
    12181 cagtatactc aactaacaga tttatgtcgg ggtttatgac ggacgagaag ccaatgaaat
    12241 atgtaattgt gtaccctttt atcaaagcgc gagagtggta tttgcttcct tttgaagaac
    12301 ggaagaaaat gatggaagag catataacag tcggaagaaa atttccgcag gttagactaa
    12361 atacatccta ttcctttggc atcaacgatc aagattttat gctcgccttt gagacgaatg
    12421 atttaataat ttttcaggat ttgatagtcc agttgcgcga aactggtgtt agtaaatatg
    12481 taattaagga cacgcccatg attgtctgcg tttataaggg catggaagat atcgtcagga
    12541 gtattggata gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn cccccaccgc agcgctgaaa gaatgggcag tagtgtgtaa
    12841 agcgctcgag gaaggaaggc aggtaattct tcttcgtaaa ggcggaatta tggaatatag
    12901 acagggtttt gaggtaaagc ataccgattt tttcctttat ccaactttcg aacatcaatc
    12961 taaagaatcc ctccagccag actacgtgaa caaattagat atagtactac agaacgctcc
    13021 ggtgaacaac aggaacagaa taacttcttg tgcagcagta gtgctagtta aggaaattac
    13081 caataaatcg gtattggaac gattggagaa atatcacata tggaatggtc agtatatcaa
    13141 cgtaagattt agctataacc ctaaaagacc actgaatgta gtagtattac gagtctacaa
    13201 aatgaatacc cctctagagg tcgatgttaa acctgagcta actggttgta agtcatggat
    13261 ccctgttcaa ttattgttat ctgacacaaa actgcaacag gaatatgata atcaatccgg
    13321 ctccgccata gatataggaa agccggattg taaacctgtt ttcgatgatt cagaattcat
    13381 ggagattgta acagagctcc aggaggtttt gaaccgataa atgagatatc gaaaattagg
    13441 aaaaagcgga attaaagtaa gtgaaatcgg atttggagca tggactattg ctttagattg
    13501 gtggactgcg cgtaacaaga agattgacga tgacgaagca attcgaatgt taaagcgcgc
    13561 ttatgatctt ggtatcaact tttatgaaat ggctgatatg tatggaaatg gtaaaagcga
    13621 aaaactcgtc gcgcaagctt ttaaagatat gcgcggtggc cgtggcgttg gcggcgtaga
    13681 tggagaggaa atcgtatatt ctactaaatg gggctatgac atgtatacta atgaacaggt
    13741 cggtcatagc gaattgccac aaaaacataa tgactctaaa tttctagaat atgctcttca
    13801 gcaaagctta agccggttgc aaacagattg catagacgta tacagcct
//
LOCUS       DAOR01000224            4797 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_27895, whole genome shotgun sequence.
ACCESSION   DAOR01000224 DAOR01000000
VERSION     DAOR01000224.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_27895"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1465 a          928 c          884 g         1520 t
ORIGIN      
        1 acaactgttc tcggaaaatc gggctcgttc gagtcttccc acgtataaga gattttctta
       61 tttagaataa attctgtaat agtcccttcg tgggtgtttc cttgtcggta ttcattattt
      121 tcttctttac gaaaactgaa cttcattttt cctcctacct ttggctccaa aacggcttga
      181 tctgggaacc aatttgttaa ttcatttgga tcagtaatag ccttgaaaac aacttctgcg
      241 aagcgtcaat gactatgttt ttctttattt ctattgtttc ttcttgttgt cctttccctt
      301 ctgttgttga cattaagggg tattattatt aaaaggggta tttaaaccgt gaaacatttc
      361 gttaattata aagatattat gggttctgca tggaaagcac tgtcagatga cagtgcagtg
      421 taagagaaat gctgcttttg ctcaacaaga tagatatgat acctactgta atagctgaac
      481 actttaactt tactttgcca gctctctcat ctcacctccg aatattgaaa gactcagaaa
      541 agaaacagga aaaaaacagg ttttattcag agaatagaaa tagaaccttg gaactcgtag
      601 aagcttttga ggatgtgtat gactataatt tgaagtcgtt gaaagaatac gtggaaagca
      661 tagagaagaa aaaatgaaac tatcactaaa aagataacat gaatagttgc tttagatatg
      721 tatccatgaa cttcataggt tagaattgtg aaaataatta acgctttccc cggtatggtt
      781 gaccaaatga ctgaagaaga aacgaaaaat tttaaggcaa ataataataa taacaaccta
      841 cttgttcaca tagggataat agatgagaaa ggagaccaaa tttagctcct actgcatatt
      901 agtttgatga tacctcaaac aaatatctac attatcgggc ttaagactac taaaaaagtt
      961 cataatttaa gaaaaaagaa tatcatagca tattgtattg atgatccaaa tgcaccatac
     1021 aaaggagtca ggggaaaggg aacagtcaaa atccatgaag atatcagtca caatatcccc
     1081 acagctaaaa agtttatgtt gaaaagcatc tgaagctttg acgaccacat cgtaaaatgg
     1141 cttttggctc atagaaaaac accgagttac tattcgacat ggcgaagtgc ggttccagta
     1201 gatgtctcct cctaaaatta gaaattgtga atcaaaggca gatcgcttac gtttgcaacg
     1261 ttaacgctat tcttacatac gaaacggtcg gttatgactg ggccagctta gtcctagccg
     1321 tgtgacactg attattgagc gcgcctgacc ggtttttatc tactagtcct ttgaaacgga
     1381 gaaggagagt gggagtatta tcatcttaat gtattctcgt caatcattgt cgctgacctc
     1441 agcagtgcac ctttttttta attggacgct tccatttctg attttttcat tgatgattgt
     1501 gtcatccttc ttttcaatat catcgcgatt gagacagata taaccgaaat caaagcagca
     1561 gttagaaata tcaggttgta tgatactggt gagggaaaag agccactacc gactcctttt
     1621 gcaatctcct ggtgtgtctg catgtatatt gctgcaattg ctggtccaat agagcttcca
     1681 attaagacga gtacgactga cataccaagt gatactccac taaatcgaag tggggtatac
     1741 tccattgtga tattaaatgc acccacttgt atcaacgaga ttccagcagc tactattgct
     1801 aggtttattg cgaccataaa ttctgttgaa tgaaacataa agaggctgaa aaagccaacg
     1861 gtcattatga tagttcccgc tacagttggt ttaagattac ctatctttga cacaataaaa
     1921 ccagaagaag gagcaaatac caagattacg atcataaagg gtagtgtaac agttgcacta
     1981 gctacagcat ctccaccaaa tccaagaggt tgaggacttc tcaccaatat tggtgtggtc
     2041 tgatatacca tcaacatagt aattccaaaa atcaagataa ggagattagc gggaagaagt
     2101 attttatgtg tcattaaact gagctcaatt aatggcgatg ctgctcttct ttctataatg
     2161 acgaataaga ctaatgatcc cattgacaac aaagctaatg acactactat aatctgcgta
     2221 ggatatgcgt tgatgttact actactgcta ctattcccaa tatatgtgag agttagtaaa
     2281 aatgaggcta tagtaatagc caaagttatg gcgcccttta catcaaggct tcgcattccg
     2341 ttactatgag taacaacttc gcctaagttg gcatgcgctc tactgaactc ggtatcggcg
     2401 tcagcttgca ctaaattgcc ttcttcttct actagttcag ccctcctatt atcaccatga
     2461 atgaatctat tgattattat ccaaaggcta atagcaacgg gtattatcga aagaaatgtg
     2521 gcatgccagc ctaagctttg gattatactt ccacctactg ccaaacccac cactgaacct
     2581 gcggcaaaca ttgagctgaa tactcctacg cctattgcaa gcttctcctt tggcaattga
     2641 tctcttataa ttccaaaagc tattggaaac atggatatac caatgccttg gataactctt
     2701 gcaacaagaa gaaaggatat gttagttgag actcctccag cagaaattcc tatgatataa
     2761 attatcagta ttattaccac cattttcttt cgtccgtaga tatcagataa ctttccaaca
     2821 ataggagtgc taacagcacc tgagataagg tatgccgtga gtatccaaga cgatgtagta
     2881 tacgaaatat caaaatccct gataatatca cgaatagctg gtaataacat cgtctcacca
     2941 tacattgtaa ttagaacggt acatccaaga attgcaagtg ttatccatgc agaagttctt
     3001 attttttcgg aagtttctgg caccgctgct tttgaagatg ataacggctg tgggagtttt
     3061 ttaggggctt cgatatccgg ggtgcgttcg tctttcttta aatcgtccat cacaatcact
     3121 tgctattggg atttccaatt ccatcacctt caataatggc ggatttgatc tcttttccta
     3181 gattggctct aaatctttta taatcttcat ctacctccat agctgctctc aatatcttgc
     3241 tttttcttct agatgttgat tgtaaaaaca ttttagtatc ttttggaagg cagtgtcctc
     3301 ctattccttc tcttggttct agaatattta cgttccattt agtatttaga gcctctctta
     3361 gttgtggaaa gtttatgtta tttgtttgac aatgcagata gagatcttca gcaaatgcta
     3421 tttgcagata tctatgtgcg ttttcggcaa ttttagttat ttcagcaacc tccatattag
     3481 atgctggatg cataggaatt cctagacttt tgagtgaaga ggaagatgaa ttatttcgtc
     3541 catcgtcatg aacattatca acaccactac catagaactg catacctgcc ttaagacaac
     3601 aatcacacac ccccaataat acgttgctga tttacaccgt gctctttttc atctaatgaa
     3661 taccatctat gaagggcgtg aacaacatgg aatctatgat cgagtatttc aaatactttg
     3721 tcagatagtt ctatcatata gataatctgg cagattggct atcagtccaa tttgcaactt
     3781 taccttcctt tcaacataag ctcgtcatgt aattgctcag taggtggcat taagtcgtct
     3841 agagagggca aagaaaaact aagacaaaat caacacttaa gaatgctatt acagaggaga
     3901 gtgaagaatc gcgtaatatg caggttgcag tatattttat ctgtagtgtc ttcttccagt
     3961 tgggttattt gtaattctga tatccaaatg aatttagcct gtcatctgcc tcaaggactt
     4021 gactaagtgg gaggagtagg ttcagataaa cgcctcccaa atcagcttgt ctcacctatt
     4081 gtaacgaact tttcatttag gttttgttgc aatatgtcaa gcctacctac gactcctatt
     4141 agcttgcggt catcatctac gactgggact aagatgatgg ccttttgaag gataatgtcg
     4201 attatctcgt ttatctctgt atcttgcttg actaccactg gatttcgtgt cattatatct
     4261 attgctcgaa ggctgtcaag atttttgcct tgtcttagag cccgcaaaat atcaactgct
     4321 gtaactatac ctatgacttt agcattactg tcgataactg gtaagccgtt aaactctccg
     4381 ataagaagct tcgtactaat atccatagca gtagtactct cacgagcagc gactacgtta
     4441 cttgtcatta cattagaagc cttcgttata caggtttcac ctcgaaaaat atttcttccg
     4501 atgatattaa atgtatcaca aatgttccta gtccttaaag gtcttaaatg tttggacttt
     4561 tagctgacga agctccattt atttttatct ctgcgctgtg gtataattat ttatattttc
     4621 aaatttgtga tatgaacaat tggtaaagtg cctataacct tatgaaaaaa ataaggagtg
     4681 tctagagtat acccatttac cagcctggac tataggccgt caagcaatat atgattacaa
     4741 ggttgacaaa tatgtgatac cttctggctc tatcatttta atgagtcaat acgtaat
//
LOCUS       DAOR01000225            5364 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_55108, whole genome shotgun sequence.
ACCESSION   DAOR01000225 DAOR01000000
VERSION     DAOR01000225.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_55108"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1649 a         1025 c         1154 g         1536 t
ORIGIN      
        1 ttaatcaacc aaaaaccctt gattacaaaa tcatagttga ttcagcagcg ttaggttcag
       61 tgattactcc caatggaata aagacaaatt caaatacaga tcaaattctt ctgctttatg
      121 ttgggcttaa tagcttgcag gtggttctac catttttgta tcattttagc atagccatcc
      181 taccgagcca aaaatctaac aattttctcc cgaccggcta caatatatgt aaaggaaata
      241 gtcattgttt agctcttgct ccattatttg attaataggc ttgctgttga ctcttaatca
      301 gctagttggt ttgaatggat gaaaaatatg acaacgatat gattttttat acatattgta
      361 tttatgtgat ctgttctatt atccagtctt taaactttgt aattgcaaat tcttcagatt
      421 gcttaataaa agcaggatcg tttatctccc attgctcctg aggaatggaa tcaaaaaact
      481 cttgcataca caacgtaaat ctaaaacgta aattccttgg attttcaatt attccataac
      541 gaaataggat attagcaatt tgtccaaata cggcatcata ctctctttta aatggagtta
      601 tttcgaaggc ttgatacaat attgaagtta ttgcttctag atcttgatgt attgtaatca
      661 aatatacata catgataaat tatcacagat taaaatgcaa tgcaagagaa atttgattaa
      721 gatttgcaac caagaatcgt actcgtgggg ctgaggacat ctttaattac agatcgaacg
      781 gctgagatgg ggacgctaat gcaacttcgt tattcttatt cgttattttg acggacttcg
      841 caaagatata gtggattctt gtcaggatca gtgaagaacg cgatcctgac tggcccatct
      901 tcagtgatgt ttggagagaa gagaatgccc ttgtttttaa aggtggttac atcattatcg
      961 aggttatcaa ctgtgaatcc ctatagcaag gctttctgaa tttcccggtt taggtccatg
     1021 tggtccagca ggatggagcc caatcgtcaa tccaggaccc tgaatttcag cccattgggc
     1081 agttacagct cgccgcatgt ccaaaagaag aagtaggctt ctatatacct caaaactgac
     1141 cgaaggaaca tgaattctaa ggagatgaag cgaacgcgtg tgacgagtcc gagcacccat
     1201 agccggactc ggaaccgaat atctatagta atataggcgt gtgatttatc acatactgat
     1261 atgggcaaag atacaaacca aataactgag cctgttgcca aactccttga aggtagaaat
     1321 cttgcttttg tagctactct tatgaaggat ggttggccac agatcacgcc gacatgggta
     1381 gacattgaag acggcaagat tctagtaaat actgccgaag gaaggttaaa gcagaagaac
     1441 atatctagag atgataggat agctatttca gtcgctgatc aaaataatcc ttatcacatg
     1501 gttacagtta gaggtagagt tgtcgaacaa actaatgagg gtgctgacga gcatgtagac
     1561 aaacttgcaa aaaaatacct tggcgttgag aaatatcctc tacgttcacc aaacgaaaag
     1621 agagttataa taaaaataaa accagaaagg atccactatc agccacctca aaattaattt
     1681 tattatcatc taaaaacccg ccgtcgaata tttccagcta tcatgatttg actcgcggta
     1741 ggctcattca ggttacactc tatgttggcg aataaggcgt aatccaacgc agctaaatgt
     1801 atcggcatac gccgattatc atgtctataa acaggcagac tccctgatcc ctgaatatgg
     1861 aaaagcggaa gagcttgctc agaacgcaat catttcccaa aaattctaac attcttgcgc
     1921 attcttggtc atagtggctt catttttgac cgtcacgtga aacgtaaata ttattgggtt
     1981 attgtcatgt catatttagc tttagtttga caaatagttg tcagatgccg tgaggcttca
     2041 agaatttgga gtcgctgttt atagcccgaa tctattactt tggttcgcgc tttgaaaccc
     2101 agcagatgaa cctactgacg agggttaccc gatggcaggt gacgacgaat gttacctatt
     2161 gcggcctgat gaaaccaata gtgttggttg gcccgaaaaa aaggaaacta cattgattcg
     2221 tctatgcgat agagcagcca gtgggggatc atttgaattg aaataatttt ggtgtgcttt
     2281 tcataggatg ctcctcaggg acgaatcgcg agtctcaaat atataaccgg gcagatggat
     2341 ctaataatag tagagttcag atagcaatgc caagtgttcc acgagtacaa aacaaattaa
     2401 aaatgctgat tgcgatagta atatttgttg ttatcgttat tgtcatgttc gaatcagtag
     2461 ttgtagtaca agcaggacat aggggaattg ttctgtatgt gggtgcagtc gaaaaccgtg
     2521 tactaggaga aggaatccat tttatcactc catttgctga acaggtaata cagatggaag
     2581 tacgtacttt gaaattccag gccagtgcat ctgctgcttc aaatgacctg caggaagtac
     2641 agacagtaat agctttaaac tatcatatca gtccgagtca ggcaaatatc atttaccagc
     2701 agctaggtgc tgattacgcg gacagaataa ttgcacctac tatacaggaa tcagttaaag
     2761 caagtgtcgc caaattcaat gctgaagaac taataacgaa aagggctatt gcaaagggtg
     2821 taatagctca gactattggt agtacactat cagcaaggaa tatagttgtc gagacagtat
     2881 ttataactga ttttgcgttc tcacaagcat ttgctggcca aatcgaatca aaagtagttg
     2941 cattccaaaa gtttctgaca gaacaaaata atctaaaagc cgtacaagta attgcaaacc
     3001 aaacagttgt tcaggctgag gcaacagcaa gagcaaatgt cgccaaggct aatggcgagt
     3061 cacaagcaat caaggtcatt actgtacaat taaaacaaag tccagattat cttcattggc
     3121 tatcaattaa caggtggaat gggaaaatgc cttatgcgct tgcaagtggc ggtggatcac
     3181 cttttttcca gctaccagta acagctgcgg cgcaacaaaa ccaaacgaaa cgataatcag
     3241 atactttagg aaaataacgc tactagctgc acttatagta tttcaaaaac tgtaatacct
     3301 tctcctcggt attattgttt tggtgacaat cataggagtc tgatatcggg tatatcgaag
     3361 aatcggggta ataggacgat atagttcttt tgtccacaag tcttaataga atcaggttta
     3421 gccgagatat atggaaaata cgcatacagt tagcagttat ctaattcaaa aacttcatga
     3481 ttatggtatt aaacacattt tcggggttcc tggggattac atacttggct tttatgatga
     3541 gttgatccgt agtaacatgc ttaaggtcgt aaacacatgt gatgaacaag gggccgcatt
     3601 tgcagcagat gcatatgcac gtgttaaggg ttttggggcg gtttgtatta catattgtgt
     3661 tggtggtctt aaggtcgtca acgctactgc ccaagcgtat gccgaaaaat cgcctgtagc
     3721 agttatcagt ggtgcacctg gagcaaaaga acgtactaag aatccgctgc ttcaccatgt
     3781 cgtccgggaa tatgacaccc aacataaaat atttgaacat ataacggtcg cttctacagt
     3841 attaaacgac cctagcaccg ctcgagccga aattgatcgt gtcttgtcta tggcgtttcg
     3901 atataaacga cctgtctata tcgagttacc tcgcgacatg gtctctatac ccatagccaa
     3961 tgtctcgaac tataggaagg acgaatatga agaacgaagc gatcctatga ccatggctga
     4021 agcagttgaa gaggctacag caatgataaa cgcttcacga aacccggtta ttgttgcagg
     4081 ggtcgagata caacggtttg gactacgaga cgaagtgctg cggctaatcg aaaagaccgg
     4141 cattccggtc gtggcaacta tcttgagcaa gtctgtaata agcgaaaatt atccgtacta
     4201 ccttggctta tacgagggag caatgggaga tgaccgggtc cgagaagttg tagaatctag
     4261 tgactgtttg attttactag gcgcgctgat gactgatata gattttggaa tcgcagtcac
     4321 accaatagat cagagtcata tcatatatgt gacaagcgag aagctgtcca ttcggcatca
     4381 taattttgaa aatgttggat tacaatcctt cattcggagt ctgagatcgg caaatataac
     4441 gaggagagaa cctctacatt acaattacag tgatggatcg gcttctacgt cctttttggc
     4501 caagatcgga aaaaagatta ctgtacaacg cctatttctc agacttgcct catttataac
     4561 cgcggatact atcgttattg ccgatgtggg agactctctg tttggtgctc ttgatttggt
     4621 tatacaacgc gacacagaat tcctagctcc tgcattttac acttcgatgg gttttgctgt
     4681 tcctgctgct atcggggctc aactagctaa tcccaaattg aggccactcg taatagttgg
     4741 agatggtgct tttcagatga ctggaatgga aatatcaact gcagtccggt accaattaaa
     4801 cccaatcgtt atagtgctaa ataatgacgg ttacgggacc gaacggcgtc tgcttgatgg
     4861 tccatttaac gacttgcaac actgggagta cagaagcatt ccaggaatag ttggaggagg
     4921 aagaggcttt gtcattaata ccgaagatga gtttgatatg gctttagagg cagctaatca
     4981 atattcggat ggcttctgta ttttggacgt acgattagac ccgaacgatg gatctgctgc
     5041 tctgaaacga cttacagatg tgctaggtaa aaaagttaga tagctgataa caatcgttca
     5101 tactaccatc atttgtggcg gctacttgta ctaaatgcca tgactggttg agaaacttta
     5161 actgggccca agagggctac gactgcctga atttagacat aatgcgacga tttaatatat
     5221 aatataccag aattccttat tcccttctct aaacaagaat atagtattga tatggtgcag
     5281 tttaattaat tttcaagtga agcgtgcttt ctgacggctc ctgaattatt attcttgctt
     5341 cttaaccgtt ttttgtactt cagc
//
LOCUS       DAOR01000226            3121 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_100520, whole genome shotgun sequence.
ACCESSION   DAOR01000226 DAOR01000000
VERSION     DAOR01000226.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_100520"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1097..1226
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          893 a          554 c          583 g          961 t
ORIGIN      
        1 gagcaggtgg ggagtagatg aatttcacta gttaggagtt ctagtaattc ttcgatgcca
       61 ggattgtgac caactaccag cactctcctc tggccattgg gtaggccact aaggagttga
      121 agatatgttt caggtccagc tgcataaaga gatcgattca aagtaacctc tcctttatac
      181 ccgcaggcct tgactactga ttcaaccgtg gcagaagctc tcatcgcagt tgagcttaat
      241 atagcatcag gaatgagttt ttctttctga agaatctttc caatgcgtgg tgcatcattt
      301 ttaccgcgtt tgttgagggg tatatcatga tcattcaact ctggatactt ccaacttgat
      361 ttggcatgac gaaggataag taacgatttc acaatgctgc ttttaacggt gttacctaat
      421 atttctaatt cccgaacaaa caataagatt tgatacattt tgaaatatat tagtcgggcg
      481 tgatttctag ataatttgaa atactgggca cgaaagaaaa cataatttgt ttcagaatat
      541 tgctgaagtc aagagaatga ctgagtgttt tcccatctat cccttggttc cttcctaacc
      601 tgaacatttg ataaagccgt tagaaatatc taattttaga aggagaatag accaaaagac
      661 cacactacta tcattggccc gcggaccccg gaatacaacc aagcgctcgt taaaagcatc
      721 agtggttcta cttgcggttt attaatttat attattctat atgcaatagg atatattcac
      781 gttgtgattc ccacatggtt gcacgcgtgc ccgttcccgg tacaaatgat atcatctgtg
      841 gggctgctgt cacaacgtga atacttttca aattgaatta aattagaatt tctactttac
      901 atcgtacact atcgaagtgc gagtccacta accaagaaat aagctgatta caacagaaaa
      961 agaccaaatc catgcataaa ttcgattaag tcgggtccga aactgtccaa ccatccaaaa
     1021 aaatcataaa gttgaagaaa ccgcagtatt tacattacac tatgaaaaaa aggagtgagg
     1081 attcttgtca gccgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnncgct accagtcata gactgcattt ggcgcgaagt
     1261 gtatcagagt gccagccgaa ttggttacag caggtaatat gatcttgcat taattgctat
     1321 tagacattat ttttcccagc cacattgagt ggttcattat tggaacttgg cggtagaggc
     1381 ataaattggg tcgaatttgg acacggtgaa actaggttca gcagctgcta caaaatatct
     1441 atactctcag acttagtaaa cagaggttgc aaaaatgttt ggtgttacag atcgagaggt
     1501 aaacgaatta gctgtggtag tagctcaaag cgcttttgga gtaatgactt gcatgtacag
     1561 aattacgatt acaaagatct tgaacagata ggggaacata tgtaaaacaa aatagcattg
     1621 tgtgtgcaac ctattttggt gtatatactg aaggaacaga attcatttcc aactcgccaa
     1681 tagtcgctgc atagatttga gttaactgcc gtgtcacaga caagattttg ttaattatat
     1741 tagccagttt accatagaat cagtaacaca tcctaataca ttagaggaaa catccaaaga
     1801 aggaagagta tcaaaaatat aatagggatt aaatagttgt ttgttttagg tatagagaaa
     1861 gtgaagttgg aagaattaag attctattca tttcctatgt cttgaccctt atttgtcatc
     1921 tcttcatata gcaataatat tccatattca tagattgaaa cgcttacgcc ccttactgaa
     1981 ggctgagaaa caaattcgtc tgcttgttgt gctgcggttt taccaccttt ttcactttta
     2041 aaatgtttca tgtgttgatt ctttgtcatt atcatgaaat atgcattcct ttcttatttt
     2101 tcttcctctt cttttatgcg gtaggcaatg cttttgattg atcgggtaag tgttttaatt
     2161 tatctacaaa gtgcactgtt ccctcagaat gctttttatt tatatgatta tttggaaaag
     2221 tcacattaac taatggaatt agagcgctag caacaaggat cgtcccaaag acactctaac
     2281 cagaaattat aactgcgtcc tatccttatg actgcaaagg ccgtgagggg ttaggtttac
     2341 aaatatggta acctttctca atcctgtaat tgcatacaga acatattttt tgaggtctgg
     2401 ggatatgcgg ggtcctcatt atatttccat ttcttatgga ttccctttgc ttgaatagtg
     2461 agtttagttc aacaagcaat tcaggaatat agcatttagc ctttctgttg tgcatacgca
     2521 attgaactct gcgtatttta tggctaatat ctctaagctg tttctttttt atgtgacgcg
     2581 ttcgatcggc caatggtgtt tctatagcaa ctgctcaata atataattta ttcctcagca
     2641 aatcgctata tatatagaaa ttgatcgttt cctttcgttc tccgatttcc tctgtacgta
     2701 tcgcagtgat tgtcattgtt gctgttaagt accggcttag agactaatcc gagaaataca
     2761 aagggcatat agcagtagaa cattattttt ccgttatttt tggcgcaaat tcttaactaa
     2821 tcgtttgtgt gagtagttgg tgtaacagca ctttgcatta cccatcataa tgtctgtata
     2881 agcgcccaaa attggtagac cggttctgaa attctccagc ccaatgaaga atttatccgg
     2941 tgggagtcta agaaaattag ttacataaac gtcgagtata ggtaattatg tctttgacat
     3001 tgcggattca taagatactc tttcttgccg tagatacagt catcctaagc gataaatgcc
     3061 taccaaataa ggatcaaaac cattgactta tgcctgtgtt agttgtgctg cttccacaca
     3121 t
//
LOCUS       DAOR01000227            4570 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_50603, whole genome shotgun sequence.
ACCESSION   DAOR01000227 DAOR01000000
VERSION     DAOR01000227.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_50603"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1499 a          862 c          807 g         1402 t
ORIGIN      
        1 tacctatgtg tgtaagtaca acagctaact atcaatattt agataagcaa aatctggcgt
       61 gtacctctcg ttgacactgc caaacgtaac gagatattca tcttcgttca tttctctatc
      121 agttcaactt gattcacctc tagaattagg gtaaatattc attgtcagta aggaagaaga
      181 ggcggaagaa aagattctcg tccgaatata ctatgctctc aatccttgaa tatctttatg
      241 tcaatgcaca gaatatccct gtaacgaaat acaagattct taccaataat ccagagacca
      301 gacagagtca ataatataat ggaaatcctt gagcaaaatg gatacataaa atcaataaaa
      361 gcctcttcaa ccgtgacatt ttatcaaata actgggcatg ggatagaagc ttattcaaaa
      421 tggattaaag attatctatc ctttgctaga actagtgcca atgcaagaga ggagtgagat
      481 gacaaaagac aacgtagtga ttattgggtg gtagaactct ggtctgtgga gtgtaatggt
      541 ggtgctggcg gagatgatgg cttatctata agcaagagat gatttatcgg tagtcctcct
      601 cttctttttt cggatatgaa tttacttact gattctttgg cctctgggct tatgtcttca
      661 cctactatta tcccgagacc aacttgattt tttgacacaa aagcatattt cgctagtgaa
      721 tctacaaata actctacatg gcgtctgtcg acgtaaccgt ccacaatctt aattattacc
      781 gccccataat cctccaaaac tttccttagg ctattaccct tattcttatt atcaccattt
      841 aatagatgac ttgagtctat gagtatatcg tatgtacttg ttataccatc accaatagaa
      901 atatccaaaa actccctcaa atcagatttt tctgacatta tatactcttc caatggttcg
      961 ctaatttcat ccactgactg cgccagagca agtattgctt cttctttgcc tctcctagtc
     1021 atggcaatcc tcatggctgt tgcaatggtg tttctttcaa acatatcggt ccatctatct
     1081 agtacctttc ttgaccttag taaaagtaag accaatggta tcagcacagc tatcaatgct
     1141 attaataaga aagtatcaac tactctgtct atcccacttg aggaaaaata atctaaaagt
     1201 ccaagtatgt taagtaatga gagcgctgcg atggagatta tcaatcctac aacaatgtaa
     1261 aggtactttg ccacagaatg aatcaatcta gctgattctt gaccaaatct ggtagtcaaa
     1321 attgttgacg acgacaagtc gtaatcactg gatctgctat ctctcatatt ccctaagact
     1381 aaagaaaaga tattgtattg taaatatata aggtagcatg taacttagtt ataagaagat
     1441 ataactaagt tattatagtg ttaatataat tccattcggt ctttattttc gtcgaacaca
     1501 tgaaaaaacc cctggtcgtc tacttgatag ctggtctaat tttcgctacc gcagtagctt
     1561 acttgatagc tgcttctttt ggtgaaagag tggaaagatc aaatcatgaa gcgaaagctg
     1621 cacttgaaac aggtcatgaa agttcagaag taacaacacc tcagcctcaa actacagaaa
     1681 ataatcataa tgtctcaggc agccaagtaa aacaaacact aaacaataca tcgtctgcta
     1741 aagaatctag tagtcaaaga aataaggaag ccacaatcag caatggtaac gaatctgcaa
     1801 gaaggagtat agagtttcct ttattcttga ctgcgggtac agcatacgca atagtagggt
     1861 tatggatggt gatagaaaag aagataacaa aatttccata catcatctca ataggaggtt
     1921 ctctattatt gattgggctt tacactttat ctcacactat cggattacca ccaataggtt
     1981 tagagcaaat aggactgtta gacttaaccg ttgcatcact acaggttgcg ataattgctt
     2041 gttgtgggta tgtattaata tcatcaaata aaatattcac aaaatctgct gaacaccctc
     2101 tggtgtaacg gagggagcga tggatatgaa taggggatat gataaagcag aaaaaaaagt
     2161 gcttgtttct tttgctgtta ttataacagt tatctgtatt ggagtcctat ctgtatcgtc
     2221 tcccaatcaa ctgaatgcag ctgtgataca atcatcagaa aatacggcca acaacaataa
     2281 taaactctct ttccataatc tagtacaaga aggttctcca ttcttaggga agctttctgc
     2341 tcccattaca atcgtagaat ttggtgattt tcaatgtgac agatgtgcaa ggtttgctaa
     2401 atatacagag ccacagctaa atcagactta catccaaaca ggaaaagtga atttggtttt
     2461 caagtatttt ccaatatatg gcccagattc cactcctgct gccatggcag ctcaatgtgc
     2521 caatgatcag ggaaaattct ggaattatta cgatacattg ttcaaaaacc aaggacctgc
     2581 aaactttggt tgggccagca aagacaatct caagaagttt gcctctcaaa catctggaat
     2641 agatatgcaa aagttcaatt catgttttga tactcaaaga tatctgtcat ttgtgcaaaa
     2701 ggactttgct tttgctactt cactgggact tcaaggaaca cctacttttg tgattgagaa
     2761 gagtgatggc tctaattccc aaactctacc tggtgcatat ccttttccgt catttaaagc
     2821 aattattgac aagaacactg gaagctgatg atgaatcaag aatagtctaa gattaataaa
     2881 aataataaaa ataatatcaa tgactactca tgctagttta ctatcaataa ttacttcaac
     2941 taaattggtc ttttgtaggc ggccatacgt tatattaggc tgagctgtta cgacaatttt
     3001 ctggattatt tttaatgttt ttgatcagct gtttttcttt tctccagtag tgacattcta
     3061 tctacctgag gacgcagtag gtataagcag gaagttaact agaagctgta tcctaaacta
     3121 tgacctttca agtaaaaagc ataattgagc gtggcagaaa aaggatgcat gtctgaagaa
     3181 cccttagact gaatcattcg taattttgaa caacattgtc catatgtagt attttggagc
     3241 ctcttattat agcgttacag aaattgctta aatgttaatt aagctattct tgtaatattg
     3301 tcagatccgc aaatgatttg tcattatctg gatctgtata cccgaatcag tagtaatact
     3361 taaggtcacc ccttcagatc gcaaggttta ggtatgactt gggaaccata tagttttcaa
     3421 accaaatatg cctaaactta tatgcgttcc ggcaattttt tttctgatgg tggttatcgg
     3481 tattccagaa gcatatgctg aggacattat tacaatcatt ccatgttcaa gcaatcataa
     3541 caatcctaga tttttcgata atccttcata ttatgtacaa aaaggacacc aaatacgttg
     3601 gtataacgct gatgacgtaa atcataggat agtcatatct acttctgatg caaaagcggt
     3661 gttgtctgat tctggcataa taaaatcaaa tggttccttt ccattcatat ttaacaatat
     3721 tggcatttac catttttcgt ctcctgtcta tgcgtggatg caagggaatg tttctgtcac
     3781 agacgatatc tcttctgtaa caatgactaa tccgaagaac aacgtggatg tacaacttac
     3841 atggactcca gctataccaa gggtcggaca aatcacggat tttaaaatta tctttattga
     3901 cagaagcacc gacaaaaatc atcagcacat agactatgta ttttcaattg acagtccagc
     3961 gaataagata ttatatcaac aggcacttca ttcatcgtgg ggagttgaat atgctactta
     4021 taagtttaac acaccaggta tcattatacc taaaatcact attgacgcca tactctttca
     4081 accagtcgaa ccagttgagg atgatttcaa aatgtcagta aaagcttgat tttgttgtta
     4141 tgaaagaaaa gtagtcgcgt aatgcatcgc aatcctgttt aacatctaga tatatcaagt
     4201 ccgtacaatc atgctagata atcctcctta ggatcaaaaa ggtagcaatc ccggcagcaa
     4261 caacaataac aatgataatc caaagattgg aactcccgtt agtattattc agagaaaaag
     4321 aaaagagtgg aacaaaaact ttgaatgatg ctaataatgc aacatcattt gaatgtgagg
     4381 ttatccttgt aatgacttga tataatcccg tatcaggaaa caggtacttg atggagaaaa
     4441 caccatcagg cgccactata ttagtgaatt taaagattct ttcctgacca ctgctattcg
     4501 taactatcac gactcttgcc aataaatctg gtaaaggttt accagtttgc aaattttgaa
     4561 tactaaattt
//
LOCUS       DAOR01000228           13560 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21335, whole genome shotgun sequence.
ACCESSION   DAOR01000228 DAOR01000000
VERSION     DAOR01000228.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13560)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13560)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13560
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21335"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1365..1437
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10088..10225
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4116 a         2515 c         2549 g         4169 t
ORIGIN      
        1 agtggaggcg tcctctggac atcatactca ttttaaacat agcaataaca acaatttttt
       61 ctcaacatcg ccttcagtag ctgtctgctg tggttgggat aataaatttg caggtggaca
      121 gttaacctac aacataattg gtggtgatac atcctctcgt caagcagtgc tagaagctat
      181 gaatgaatgg acttcaaatg tcaatggact acaatttact caagatccag ccaaaagcaa
      241 tgcagacata gtagtgaatt ttcagaatgg tggtggtggc agtactgcta aacatggtag
      301 tggcataggt tctatccgta gtagtggaca ttcatatagt gaaatattag gagagacaat
      361 aataagtgcc agtagtggcc ttatagataa tgcccagata accctagcca caacagggtt
      421 tggttctcct cttgatacaa ctcagcttaa acaggtagct ttgcatgaat taggacatgc
      481 tctcggacta ggacatgcta attttgttgg agatataatg gctccagctg ttaactatca
      541 aacaggagcg atatctaaat gtgatattaa tgctgtgtta gcagcaaatc aatggaagct
      601 tgtagattca ggcactagtt cacaaccacc acatttagac catgtgaact gttgatgatg
      661 atattttgat tccataggca atcttattta aaaattcaac aataaatgct ttgaatatga
      721 ttatgactat gatcaatagc aatattatga agttgatcga aaaacctcat cgcagaggga
      781 atagcaggtt aatagttccc accttctaat gttagctcaa aaaataatga acacgtggag
      841 gtaacaaatt agttcacgct gagcacagca gcttcaatct tgtgtaatac tgtattaatg
      901 taataatttg taagtaatga tggtacagat tgtatctgta attggtctgg ttttaggaga
      961 attgaattta tttgatacag cagctcaatc ttcttattca tatttggttc atttttgcaa
     1021 ttttcgacaa gttgcaatat cgtagtatga taatctgcga ttattttgtt gttattttgt
     1081 attaacatct tctctaaata attattataa caggcattcg atatatagtc taatacaact
     1141 tgatgctgat ttcttataaa gtctgttaag cttatgtatt tagcgtcgat ctatcttgcc
     1201 atcttaggtg ttgttccata ttgttgtgat agatctttgc cttaactcca agatttgtgt
     1261 atgattagcc ttctgtatca acgtcagcag atgtaacgaa aaaacaagct tgaatcattt
     1321 tacgtacctt cccgcagaag cttttgtatt atatgatata aagannnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta
     1441 ttatatgata taaagaagaa tgaagagaag aaaaaaacat ttgcccataa atcccaaagg
     1501 tctagtgcca actaattacg tgtaccaacg aatatccaga tcactgccgc cgccaccaca
     1561 acagaaacaa gacaaggccc agactgcctg ttatcatggg aattagactt tggcatgacc
     1621 ttaagagaat tgcagatgat tttgtctgca ttcaccataa gtgtaaacat gtattatcat
     1681 ttatctttgt aacagacata atactttgca tgtcattatc taacaaagag cgtacggtgt
     1741 gattctcttt tggatgggtg ggatgcggct tctgtgctct tctttatcgt tcagtattat
     1801 tacacttgtt tttccgtatg ccgaaggctt gtgaaatgta aatcaaaata atcaaattgc
     1861 tctttacgga aagaccttga gattccgcat aagattgatg tagggagaac aaaggagaat
     1921 ctatctcagt gccgcaaaga gaatatccaa gtgatcgaaa tcctttgaca gaaatgatat
     1981 ttgttggacg acgacaataa caattcaaaa agccattttg catttgtaaa ggattaaatg
     2041 tcacattttt ttactattcc aataatagaa aaagtatcta cagcagcact tatgtcagaa
     2101 aaacaagttt ctcagcttat tgttgtcgac ccagataatc aaaataagtt agcaggcata
     2161 atatcggaaa cggacatatc aagaacagtt ccagcgttta aatcaaagac aataagataa
     2221 gtttatgata atatggattt actgttcagt gctaaatcaa agccatccta tgttcgaata
     2281 gaagatataa tgactcgaaa tgctataacg atagaaaaga ggccgatttg acagatgctg
     2341 caataataat gataaaacaa ggaataagtg gactacctgt tattgaatca ccagataagg
     2401 tggaacaacc catcggagta atttcaaaat ctgacatagg tagggcttta acaacgaaag
     2461 agcgagcttg ctaatggacg aaagtaggcg aatcgaccgc attgaaattt ccgatacacc
     2521 tacagatata agagaattca tgcaagacat taatgacgga tgtacaagtt ttaaaggtta
     2581 ttgcagaaag tagagccaga gtcactaaag cattaaaaca agaaagcaaa caatttcatt
     2641 catatctaga aagttcagat gaattccttc gctctcacct agggtctaga gtgcgcctag
     2701 tgattctata tgccgatcta gtcaaatcta ctcgtatgag tatgatgctt acacttgaaa
     2761 actgcacagg aaatgactgt tgttgttgca aataaccatg gctatatttt gaagtatgtc
     2821 ggagatgctg ttatagctta ctttccggtc gatgttgctg ctgattttaa agtgtccagc
     2881 aagatgcagt ttcatgtgct atgaatatgt tagttgtggt agaacagggg ataaacgatg
     2941 ttctcaaaga atttaatttt ccagatttac atataaagat tggaattgat tcaggagaaa
     3001 atgcaattat tgagtatggt tctactggaa cgagatccca tattgacatc cttggatatc
     3061 ctatgaatgt aactgccaaa atcacgtcgt tagcaagacc agatcatatt atgattggtg
     3121 ataccacata tcaaggtctt gatttacgaa tacgtgaaag attaataaaa ttggggttag
     3181 agcgtcaaga ctatattgat tatcgaactg gtgatgcata tattatatcc tcatttggtt
     3241 tttagaccca aaataagagg ggctcgatag gggcatagcc tgcaaagcta tactccagcg
     3301 gacctgcctg atccgcatat cgtcacttcg ggggggctgc tgcggtatcg ggcttatctt
     3361 taccatgatc aacatcatta ttacgtgttc tagactcagg atttaggtct aacgagtttt
     3421 gccatgaatc tgatttctcc accttcatct cggatcggat gtggtcttat agtatttgag
     3481 tttaacatgt ctttgctttg actcactgcg ttgtagccct tctcaaagat agtcgtcgca
     3541 cccgaatcaa tatctgaata gctgatgact cgtatcagta aacctaagcc aatccatttc
     3601 ccaaccttct atcattccgt tttgttcatc agctacacat acttgggatg taatatatac
     3661 tagtgaagtt gcaaacatac cagatgaaag ttagcagctt gtattcgcac gtggttttgg
     3721 tcttctattc tatggctgaa tttatgtcca atcaagtccc tctgatttgc tagcgaattt
     3781 gtcctcaaag gcaacttctt aacgtactaa accaacggat gatgtttcat tcttagcaca
     3841 taacttctag ttgctggtat ttcgcactga gattagcatc acatcgttat tattattagt
     3901 gtaagtacat gatcaagata acaatacatg tacgtaaacc tagcaagaaa gataccttta
     3961 gcaacaacat ctctgattgc gataaatatc gtaattcttg gactgggctt actatcaggg
     4021 tctcagttac aaatcatacg tgattatggc tttattccga ataatctatt tcatattaat
     4081 aataataatc tattatctga cgtcctaaca cgattatttt cctcaatgtt tattcacagt
     4141 ggtatagccc atctggcctt caatgtatta gcactggcat atcttggtgg atatgcagaa
     4201 agatcagtgg gaattcccag gtacgttctg atttacatcc tggctggtat tgcaggagcg
     4261 ttattccata gtgtgattgc atcttccata ttgggtagcg gacatgtaat acttataggt
     4321 gcatcaggag caatttcggg agttttaggt atcgctgcag ctgctggaaa tactagagca
     4381 tactactggc ttatgctaca aattgtgttt gctgtgtttg gttctattgc ttcaattcca
     4441 atagcattta ctgcacatat tggtggtttt atcgcaggag tatttctcac taaattattc
     4501 gtaggaatag aacgtaagaa acgagcaaaa tacttgaggc cttcgggaaa taataattat
     4561 gatacgtgga ggggagaata gtcaacagag gagacgtgag actttctgca ctaacgtgct
     4621 ctgttaattg gtttgtgttc gaatctagaa ttaactatta aaggaaatta ctttatagca
     4681 tttttaccta tcattaaatg accaaacaaa aaaatccaaa aataccaaag gaaagaaagt
     4741 gaaaaataat gaaatgtgga ttatatcaat agaaaggtct caatcctaaa gacaacagta
     4801 ccacttgcat ttaagatggt atttcctgtc tatattgcta tagctgcata ggattataca
     4861 tatcgttgaa acttttcaat cttcttcatc gacgtctttg tcttctaatt cctcttgttc
     4921 ctcatcttgc acctcatgag tttcatcctt aatttcttct cttgtggttt ctttgctcat
     4981 tttatttata ggtgtcaagg taatcttata tattcttaca tatcgttatt cataagtcgt
     5041 ttaacagagt ctttggttgc atcaaggtta tgagattgca ttatatccat ggctagcaag
     5101 gcgttgtatt gcattgcatt gctttgcatt gctttaccca cccacttcaa aatccaattg
     5161 actttgagat atgttaagat gagcatgggt catagtatgt gattttacac tcttgacctt
     5221 ctagataacg ttgagacctt tttatatcaa gaagcttact ccaggtgtat gaaagttggc
     5281 atcaccttac ccaatctcgg tccacaagca acaagagaga atgtccacaa gttggccact
     5341 caagctgaga aagacggttt tgactcgctg tggacaatca caagaattct atggccatta
     5401 aaaccacaat cagcatatgc agcaactcct gacggcagcc ttccagttga atatcaaaat
     5461 gtattagatc cgttggatgt tttagtatac gtagctgcta atacaaataa aattgcatta
     5521 ggaacgtctg tagcagacct attcttttat acgcctatta tgcttgcaaa aagatttgcc
     5581 acattggatg tcttatcaca aggaagggta atatctggcc ttggtcttgg gtggtctaaa
     5641 gatgagtatc aagcttctaa tattccattt atgaatagag gagaaagagc ggacgaattt
     5701 attcgagtaa tgaagaaaat atggacagat gatatcgtag aatttaaagg aaaatactat
     5761 gatattccca gcttcaaaaa ttgatcctaa acctattcaa aaaccacata ttccaattta
     5821 tcttggaggc tttactccta atacgttttc aagaatagta aaatacgatc tcaacggctg
     5881 gctgggagta gtcggaggtc ctttggaata cattgaaagt agtataaagg cgataaaaga
     5941 acaagcaagt caagcaaata agaaccctga caactttcga atcataatga tgatatatcc
     6001 caatgtcaaa gagggtatat catccaagaa tgataaaaac agatttccgt tatccggaac
     6061 tattgacgaa ataggtaacg atatacaaag agtaaaaggt atgggagtag atcatataat
     6121 atttggttac aactttctcc caataggaag agatatagct aagatccttg atatctccaa
     6181 acagctttct aaatttgcta ggtaagtcac tctctatttt atatttgcac cctctatttt
     6241 tgtggatttc tcattgttca acttattatc gttattgaca tacctattat cgttagaatg
     6301 aatactccta attacgaact catgcatgtc cttgggatct tggatgttct gacattgctg
     6361 tcggtcctat cagtatacat tatatccctg gccctgtacc acgctcccag catatcattt
     6421 gggatttcct cctggtaacg aggaaataaa ttaactatgt acactttctt atctaatacc
     6481 ggagatgaat ttatcacctc aaccaagggg tattgctaag aaggcttcca tcccataagt
     6541 acctgccatc atatggctat tccataaaat ggaaaaccta tgcacccaat aatatgatcc
     6601 acgtctatgt cgataaggtt gctgtcgaag actacggctt cacttgaatc agtagatgtt
     6661 acaataagcc tagcagtgct aggattagaa ccttctctct tcctaagttg ttcaaaatca
     6721 acatatttgg aaagtgtttc tttatgcggc gaaatatcaa atagcgggta aggtaagccg
     6781 aatgctggat aagtaaaact ctctggcttc agccatttag gaataaaggc tttcgggtta
     6841 cctcatgtag cagcaaaaat tggagacatg atgcgtctag tctccaatag taatggtatg
     6901 ggagaaccca tgcactcttg tgcaagttct agccaaaaat cctcaagctt gcgtgaggga
     6961 ttttcgtctt ggatttgatt tgcaaaatta aaataatcta gaagagtgtt gttgtgcctc
     7021 cgcggataat gcgaggaggc actaaatgga caataaccca cctgataaga ttcttacaat
     7081 ttaagaagat aaatgcagtg acgaaagata accgctggaa cgaaagcttg ccaatctgct
     7141 attacatatg attctggttg tcttcgagta ggctgtgaag aagaaagcaa gatggtcgat
     7201 gaatagtcgt cacggatttt tcatagaaat ctgctgtggt agtaaagaac aaaactcatg
     7261 ataaactgat aaatcaattc ctcaacacaa aaaaaggtct tggatttatc tagaccaaat
     7321 ttagatatac tagaggtatt gcttataaca accgtaaaca atatggctga aaaagttagt
     7381 aacgaacaag gtccggcagc gataaaaaca atacttgtag ctattgataa atcaggctac
     7441 aaagaaaaga ttatcagcta cgccgtaact ttagccaaag ctcttggcgc ggaaataatt
     7501 gtaatacatg tgattgataa atcctcatta ggtgcagctg gggatttact agggtattac
     7561 agaggaggaa atatagaaga atacgagaag gagatgaaaa agaagggcga aatactctta
     7621 gccgaagtag aggcaaccgt taggaagaat ggaaatatca atgtaaccgg ggctgttctc
     7681 gtagattcat ctgtggctaa cgcaattatt aattacgctg agaataaaag agtagatctt
     7741 attcttgttg gtgctagagg gatggctgga atcgaaaaat tcattttggg tggtgttgct
     7801 actaaggtca tcaatcatgc acattgtcct gttcttgcta ttcgatgaca ggcaatgtac
     7861 tgttcttgct atccgtagac tatgaaaagg tatgaggtat aaagcaaagc acaggatatt
     7921 ttacaaagat gggatttagg ttatggcagc cgtatcactc gtatcctttt cacttcgcca
     7981 tctggctgtg agatcaggtc ttccaaattg ttctgatatt ctcttgtaca tgtttagctc
     8041 ttgagtcgat aacggtctaa tctttttcat tgcctcttca aagtggcgca aatggattct
     8101 tagttcttgt acatgtttat gtgcttcctt tggatcttga tactttgaga tatgttctcg
     8161 caatgcaatc ataactgcag cagatgagag tgatgcaata tcagcacctg tgtaaccatc
     8221 tgtatggcct gctaattctt caatcttaac atcctcatct aatggttttt tctttgtatg
     8281 gatttttatt atctgaatcc tggagtcacg atctggagga gctacatata gcaatctgtc
     8341 aaatctacca ggccttagca atgcgggatc tataatatca ggcctattag tagcggctat
     8401 cactatcacg ttggttaata tttcaagccc atctaactca gttaatagtt ggcttattac
     8461 cctttctgta acatgtgaat caccaaaatc tccccctctt acaggagcta tggaatctat
     8521 ttcgtcaaag aatatcatac aaggagctgc ctgtcttgct tttctaaata tttctcttac
     8581 tcctttttct gattccccga cccactttga aagaagctct ggacccttta tacttatgaa
     8641 gttcgcttca ctttcatttg cagctgcttt ggcgatcagt gtctttcctg ttcctggtgg
     8701 accgtaaagc aataagcctt tagggggtgt agcatcagca tgagtaaata ttccttggta
     8761 tttgagaggc cattctactg cctcttgtaa ctcttgcttt atagatgata atccaccaat
     8821 atcttcccat ttcacatcag gaacttcaac gaatacttct cgcatagcag acggctccat
     8881 ttctttaatc acatcggtaa aatcttgcat tgtaactatg atcttcttta atatatctcc
     8941 aggaatactt tcagatgata aatcaatttc aggtagtact tttcttaatg ctcgcattgc
     9001 agcttctttc gataaagctt gtaaatcagc accgacaaag ccatgagaga tatctgctag
     9061 tttttccaaa ttaacatctt tggcaacagg cattcctcta gtatgtatct gtaaaatctc
     9121 tagtcgccca ttcctatccg gtacacctat ctcgatttct ctatcaaatc taccaggacg
     9181 ccttaatgca ggatcaatag cattaatcct gtttgtggct cctatcacta ctacttttcc
     9241 tcttgaagac atgccgtcca tcgctgacaa tagctgggat actattctcc tctcaagttc
     9301 tccactttct tctctttttg gtgcaataga atcgatttcg tcaataaaaa taattgaagg
     9361 tgcattcttt tctgcctgtt caaacatatt acgtagcttt tcttcagatt ctccataata
     9421 tttgctcatt atttcagggc cgcctatagt atagaagttg gcattagttt cattagctac
     9481 cgcctttgca agcagagtct tgccggttcc aggagggcca tgaagaagaa ctcctttagg
     9541 cgcttctacc cccaatcttt taaatagttc tggatgtctg agaggaagct cgatcatttc
     9601 tctaactcgc gcaacttcgt ttctgacgcc accgatatct tcgtatgtaa ttgaaggaac
     9661 tccaccttct tttgatattt gagctgcctt ggcactttct tcagaaactg taatttttgt
     9721 ggaatcatta attataacgg gcccggatgg attggtcgct attactacaa gatctattct
     9781 ctgacccatt acattgatag gtatcgtatc acctttggac attaatgctc cgtttaggta
     9841 atcgacaaga tactcttctg ctccgactat tctaagaggt tcggtgggag cgagggttat
     9901 actcttggca tcttttgatt ccacttttct aacttctatt gtatcattaa tgcccacatc
     9961 aagtctgttt ctggtaaatc catcaatgcg tatcaatcct ttgcctctgt cgctttccct
    10021 tgccggccag cttagaacag tactcttctt tcgaagcgat gacaattcta aagcatcacc
    10081 tgtagacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnattct aaagcatcac ctgtagacac gttcagacgt
    10261 tccgctatat ctggatctat gcgtgcaatt tttcttccta cgtcacgttg ttccgctact
    10321 tctgctactt ttagatttgc tactttggct gtttctttgc gagtagtgtt tttgtcatta
    10381 gtatcactat ctgaagacat gtctttgtat tactagcact caataccgta gtacttaagg
    10441 gtgactaata tgttcgaagg tttgtaagat tatttacctt gcccttttgc gggggatttt
    10501 taaacttact atccaactct aatgatcacc acggtgtttc atcgtccgcg ttaacaggtg
    10561 ggagcaacat aggcgtactt gcaaaaccaa cctgaagaaa agattgtaac tcgaccaagt
    10621 agcctgtcgt cttttatata ttctcatctc ccaccataat atttaagatc atttgctctc
    10681 caaggatatt gttacgaaga agaatgccgc tactatcaaa atcataacta ggtaataacg
    10741 atgctccaat ttttctaacg tgatacgaca gaagcaaatt caactcctca acgcaaatgt
    10801 taattttaaa cgttcgtgtt aaggagatct attctactcc gcggagctac ctcgctgatg
    10861 gaacctgccg accaattcta tggggaccga agtgccggcg tgaaggatcc cgttggcaat
    10921 tattggtgga tcgccaccca taaagaagac ctatcttctg aggaactgag gaagcgtgcg
    10981 gaagagtata tgaagacaca tcaaaagggc taatgaagaa gaatgctata actatttatc
    11041 atttcagttc tggtaactta tcgcggtttg gcatagaatt atagtcgtag taagaatagg
    11101 tatcccacga ctctactcct aattacagct ataatatttt attcatcatg aatggaaact
    11161 gacgcaaagt aaacatttag gagtttgcaa attacatcga ctcacatctc agctgttaat
    11221 taaatacgtt cattcataaa caaattagtc gttgaattag agactccact ctctctttgg
    11281 tgcagttggt tctttgcagc gttgtgtatg attttagcta catatgcgtc aattcctaag
    11341 atggacgcta gatccgatgg agaaagtctt atcaatcgtt cacttgtaaa accgtagtca
    11401 atcaagagcc cctttaggcc atcagctatg gacatatcct tgatatcgtc gagtctaggg
    11461 ttataggtta aactcatgat attactgatt cagcaatgct acgtaaagtg agttgcgtat
    11521 atacttacat tagattagct tcatgccata tagacactat aacccatcta tttttccata
    11581 atcatcttca gattcgttgt atggtatgct cacgtaacaa cagaaagata tattgttagt
    11641 ctaaagtcta ggcagagtta tagaactaaa acgagaataa tcggaatagt tgcaaaacac
    11701 gtcatttgat ttgcggccag atagtacgat acatagtcat tgcatcatag aaacaataga
    11761 tcgaataata ctatgaacag cgttcaatat gaaagagtgt attttgtaat aagagtggct
    11821 atcactaata tgtatgacac tgaaaccatt caacaacaaa ccaacaatcg aaggacatat
    11881 ggcggcaatt taaaggtggt cagtcagtca gatggtagtc agaaggaagt aggagaggtt
    11941 ttgagaatgg cgctcatttg tccttctccc aagtgaaacg atacctgttg cccgttggac
    12001 acatctgctg acaacagaac aagaaaaaga atcaatttat aaattaacgc agaaggctat
    12061 atgtttctta tttctgtacg aacaactaag cgaaatgata caatacccta ggaaatggct
    12121 cacagattga tgaaatcaag ataaatctgc tcattagata aaaaaaacta atggctattg
    12181 gggctaacct tcaagttaca cctgtttcta tatagagatc aggtattgtt gttatttcat
    12241 gtgtgaaatt ctaacaatta tcgcactaat cacgaaacga tgcctccaac acaattcagt
    12301 ttcactacaa attctattaa atgcatacct tgactatgtg agagaagaga attcacactt
    12361 cagctgtgaa ctttgccttt ggacatttga aacaggagct gagtaggata accacaatta
    12421 tttagagcac atgatcataa aacactgtcg aaaaagctct tccgtgttgt atccttatca
    12481 atgatcaagc aaagtagaga gatgtccgat acggactaca cgatggtatt ccttactttc
    12541 attcttgtga ttgcgaatgt agtgcttgca ttttctaccc tgatcagtgt cacggtgcac
    12601 tactcctctt ttttagagat cgcaaaaggg aacattacga tgatctgaac gataacgtgt
    12661 tcgctaaaag tagagtatgt tgaaagtgaa agtgacgttc ttccaaaaat agtcggctcg
    12721 ataccaaagg attcgatgtt ttatgatgat gcgaaaagac atatgtcaag gaaaatgaaa
    12781 aagaatttag ataatttcga aaacaacgtg aaaagatgta acgaattcat tgatcaaatc
    12841 aatccaaaaa tcagagaggg aattaaagct atagtattcg aaccgataca tggacatata
    12901 tcggacgaag atgtttcctc aatcaacggt atagtaaaaa cgggatatta ccaagccatc
    12961 agaagcgttg acgacatagc tgatgcgata cggaaatggg atctagcctc caagataaat
    13021 tcccgtggtc ttacgggcaa ggaggcggat agagccagaa tcattctagg aacactacaa
    13081 caagaccaac caattttaaa tcatcttaga ggattacaaa aaaagatatc ggatttaaca
    13141 gcagaggcaa atattatcaa tagccaagca actgaaatat attcattgat aacaaatcac
    13201 cgctatagag caaaacatat gtgctgtcca tttcttatca aggagctatg gcagagtctt
    13261 ttttaattat atggtttcaa aaacgataac agtagtagcg tagtgagtca aatgatattc
    13321 ggacattttt gacattttca tttctttgca tatatatcta aattaaggga cgtgttatat
    13381 tttatatttc ttagatgcat tctcgattac ggtcttccac ttattttcgc cttcaatcac
    13441 aatacctcct cagcaggtgt ttttcctttc aatgcctcat gcggtctgaa ataattatga
    13501 taaatttggt agcctgacag aatctttgtg tcattattct ttaatcctct catggtcttt
//
LOCUS       DAOR01000229           12479 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_38152, whole genome shotgun sequence.
ACCESSION   DAOR01000229 DAOR01000000
VERSION     DAOR01000229.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_38152"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    6735..6915
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3847 a         2257 c         2282 g         3912 t
ORIGIN      
        1 atatttgtaa agcgtgttgt tatgcaggga tttcctgctt gcatcttctg gctccttccg
       61 aatattccat caaaaaacat taaagcaaca gcgaatacta ctttggtata cttgtagatt
      121 ctcacatata ttggtaccga agctacaaaa agatttttct ttcctgtaaa accagagcgc
      181 actaccccga acctcttctt atttttgtca gtctcttcta gttcacacgt tactacgctt
      241 ctcggaattt tttgtatatc tgcatcctct catggatatg atatcttctt attcggaata
      301 acgttctttt gctgattctt gacgaacaga caataaagtg ttcaatagtt gtcatctaac
      361 ttcttttttc tttcctttgt tttctacata ttccttcaaa gcggttcaac ttatagtcat
      421 acatagcctc aaaaaattct atgagtttca acgttcgtat tctattcaat gaataaaatc
      481 tattccttcc ttgtttcttt tccgttatca aatctgcgtc cttcaatatg cgtaggtgag
      541 agggcaggta aagtaaagtt aaagtgttca gctattttag taggaatcat atctttcttt
      601 ttgagcaaaa gcaggatttc tcttctacta tcgtcagata atgctttcca tggagaagcc
      661 acaatatagt tatatagtta aggaataccc attagtataa taatatcctt aatgtcaacg
      721 aaagaatgaa aagacacaac ataaaattga gatgaaggca caagagaggc atttgagaac
      781 aactaccgca cgacagccta tttcatctaa cttttctgta acaaaataac gctcaatgga
      841 ttaacttatc ctgtatatta atagcatctt aaagcaatat atattacagt attataagct
      901 cgtactttat gtcgagtaaa aaccttttct atttgtaata atggtttacg atctgtctat
      961 ctttcatgta actttgtcat atcgtcatct tgcattaaga atgtacgtaa tttttcatat
     1021 acgagagctg tcttctcaac aaacggtgca tgacctacat ctgctattgt ttcatatttg
     1081 gccattggga gctcctgtct aaacctatga taatattcaa ttggtattag attgtccttt
     1141 tcaccccata tgataaaaca aggaatatct tcaatttgtt tgaatccttc tggttcaatc
     1201 tgtgttgttg tactgttatc gaaagcggct tcaaacgcat gttttgcgcc tggcttttca
     1261 attgtgtaat caaacaaatc aactgctacg ggaagcagcc ttgaaggacc agcatatagg
     1321 tcctctaata ctcttttgac tttttcatat cttgttactg gatttacctc agtcgcagca
     1381 acacgataat ctttcagcaa cttcgttggc ccatcaagca gacctgagga atctatcaaa
     1441 acaagctttt ctatcatctc tttattttgt atagcaactt gtgcagctat atacccgccc
     1501 aacgagtggc ccacaatgct tatctttgta tgctccttct cctctatccc aattctttct
     1561 ataaggaaat ccataatgaa tttgctgaac cctttgactg tataatctgc attctctggc
     1621 ttttcacttc caccaaaacc tatcaaatca actgttattg tgtgaaaata ttctgataat
     1681 gcttctggaa tatctctcca agcaagagat gaggctccaa gtccatgtat aaataacacg
     1741 tgctcacctt taggatttcc gtactctttg taaaacgtac cgattccttt tacagttgga
     1801 aggggcattt gcattgaacg tcaaaattag catactgtgc ttaatatgtt tgctaccgag
     1861 cttccggtac ttgcctatgc cctgttcaat cgcgaaagcc ttcggtaaga caaacatgaa
     1921 gtttccgtca ccctgtcaat acatgaactc gtcttatctc gttttgtctc gctttttctt
     1981 tttcgacgtt tgtatcacat acattactac aggacctagg aaattgtaat aaaaatgtgc
     2041 taaaaatatg ggagatccct gaatagactt gtggaggaga tatacttgcc cagtatcctc
     2101 aagaaatcaa acaggaagaa aaaggaatag agggtactag tctagtttca gaaaatgaac
     2161 atgcgctatc gtagctgacg agaatgagat tcataatcag caagagttaa tggctatcct
     2221 taagactgcc tatgatatac aaatcgtttc cgaaaatgga gtgatatatt tattcatatc
     2281 gtttttacaa gtagggtttg taatacaatt ttacaataaa actatcatat acctaatgta
     2341 gaggtaataa ataatgctaa ggattctctt gctggcagcg gaatatcctt accctcacag
     2401 ctgcaaaatt ctactattcc aactaatttg gttggattaa atcctaccgt cacgatattg
     2461 aatgctgaag tttgtcaata tcaagtcaat tagattctga cctgcttgtc caccaacacc
     2521 atgtaggcaa tataacaaac aatcaaacat agacacacaa gaaagatttg atcatttgtc
     2581 aagacatggt acaggataac cttaaaccgt tcttcgacac atacgcaatt tcgagtagtg
     2641 gttttgctgg aattgaggtt ttaaagaagc tttggaaagg gtttcattca taatagcagt
     2701 gtttagtaag aagtggagac gccctaaaca taaacataac atactgctag taggcaattc
     2761 tagaaaaggc ggttttcctg atgtgttaaa tattagttac cagagatacc taaatgactg
     2821 aaaagatgaa aaaacagtct aaccgctcat gtgatgttct aattataggt ggtggctctg
     2881 caggtcttcg agctgcaata gaggctcatg atgccggtgc acatgttctt ataataagca
     2941 agagtagaaa aggagaccca catacagtat tagctagagg tggcattaat gcagctcttg
     3001 ctactatgga cccagaagat aattggatga tacatgcagc tgatactcta agagaaggag
     3061 aatttcttgc tgactacaac aaggtagaag tactgtgtaa aaatgcacct gatgctataa
     3121 atgaactagt aggatggggt gctcgatttc atagagagag agatggccgg ttaacacagc
     3181 gattttttgg cgcccatact tacagaagga ctgtgtttta cgaagattgg actggcaagg
     3241 aaataataag tgtattgatg gatcaagttc gtcgacggaa aatcgaaatt gtagacaacg
     3301 tttacatcac gaaattattg aaatcgcgtg gtgcaagtgg aaaaataggt agagtggcag
     3361 tagaacatat caaaggagca actggcatag atgtcaaaaa gaaaagaatt gccatgttcg
     3421 aatgcaagtc attaatcctc gcggctggtg gatatacgcg agtctatgcc gtgagtagtt
     3481 ctagaatttt tgaaaactat ggagaaggtg tagctcttgc atatgaagca ggtgccgatt
     3541 tggtggacat agagatggtc caatttcatc ctactggaat ggtctggcca aggaaagcag
     3601 taggtactct ggctactgaa gcaatccgtg gcgaaggagg tattttgcta aactcaaaag
     3661 gtgaaaggtt catgagagat tactatcccg aaagaatgga gctaggacca cgtgacgtag
     3721 tagcacgtgc aatttttaat gaaatcgcat caggtagggg tactaaacac ggtggagttt
     3781 ggttggatgt aacccatcta ccaaaggcaa aaattttgga gagactgccg accatgtaca
     3841 aacagttcaa acagcttgat ggaatcgaca tatccaagga gaaaatggag gttgcaccca
     3901 cagcgcatta ttctatgggt ggggtccaag taagtagaga ttgtaaaacg aagattagag
     3961 gtttatttgc tgttggagaa gtaataagtc agattcatgg tgctaatcgt ttaggtggaa
     4021 atagcttact ggatacagta gttttcggaa aaattgttgg aagagcagca gctatcttag
     4081 caaagaattt gaaaccacaa ttactaggac tgagcgctaa tagacaaaac ggatttgatt
     4141 atggaatctt tgtcgccaag gatccaatta aatttcgcac tgaaatagaa aagttgatga
     4201 aagatagcgc aggaataata agagaagcag gcaaactaca agaaggattg agaaagattt
     4261 tgaaattaaa gaaagaattt tattctaaag ataatattct gaaaaaattc aaaattaatg
     4321 agaatgttgt tagaacttgg gaagtaaaat ccgcaatagt tgtttgcgag gccataataa
     4381 gaagcgcttt aatgcgacaa gagagtagag gagctcacta caggttggat tttcccaagc
     4441 tagatgacaa aaaatggaaa ctaaatatct attgtaagca aaagggaaat ggcatagcat
     4501 tgtttaaacg gagagttaga gaagggaaag gacccttgaa ggattttctt aaagctcacg
     4561 tcaaagcaga gcatcatcgg gaattcgaat aatcacatag ttcgctcgta aatctgttta
     4621 ttagtagata tgtactactt ttttatacct atttggtgta atagtctgag tcatattgca
     4681 tattggtgta atagtctaag attacaaggc ttgatacatt gttcttggct cgattatctc
     4741 tttagatgtt gtcattatgg gtggatagca aacttctttg cttcggctca cgatacgagt
     4801 cacgtcgact tcatgaatac aggcccttat attcctgagc aatgtaagta cgaacttttg
     4861 gactctagtc agatcttatt aattaatgac tttttttcca atatttgatt agtctttgat
     4921 tcaccctcct taattgaatt ctttatagct tctatcgaat aatcagtatt ctcatagaag
     4981 cgtggtaaag gctcatctct aattatgcga aatatgctct ttatttctgc cccatgttca
     5041 cgtttcattt tagtataatt acgatgaatc ttttcgagct gttttttatc tattctagca
     5101 ggatcaatat ttttgtcgat tatctggtag agctcttcta tatatttcag atatcgtgtt
     5161 attactttag ccatctttat gctgtgttga gatttatcgg aaaacatgat atccctagct
     5221 ctatgataga cttcagtcaa gttgtctggt agcttatcag ttctcctagg atagttttca
     5281 acaaagaaaa gtcttttgtc aatcacaggt gaggcatcta taacctcgcc gaagggtgtg
     5341 ttacttaata gaccgccatc ccaagcatat acaccttctt ctacttgaac ccagggaaag
     5401 ctatatgatg gataaccaga agttgcaagt atatgcttag gagttatttt ctgcctagag
     5461 ctatcgaaag ttaatggttc cgatgttaag acatttactg cagtcattat tagacgtgcg
     5521 ttaggattac catcaggctg aagtttatta tagtcaatat attttgcaag agtttttacc
     5581 acaggtgaat ggtcatatat gtatgtccac cttgatgggt tgaaatattg ggggtccttt
     5641 aaagcataat ccgctctcca tctggggacg aagaacttat cattgccata aagtatagag
     5701 ctgattgtgt tcaatagtgc attaacttcg tcggtggttg gtaaatgatt ctctgcagct
     5761 ctaataagtt gccctctaga tttgtcaaat agccagtcca gtactggatg agtcaaattt
     5821 acagagttct ctgcaagttc taaccaaaac tcttctagcg tgcgttctgg atgctctcta
     5881 tctacactac cagctattat agcagcgttt atgccaccaa ccgatgtacc cgctacaata
     5941 tgtattttta tattgttatt tgcaagagct ttaaaaactc cacatccaaa tgcacctaag
     6001 gatcccccgc cttggagtac cagaacattt tcaatattat tagaagacat gaccagatcc
     6061 ccttatctgg gaagttacta acgccattat cattaccatt gaagttggtt tacagactct
     6121 tatataacat aactccctcc agattcttgg aaatgtgtta ggattagata gatatagaat
     6181 cctactaagt cgagtgcctt aggagtcgtc ctagttcacc ttacgagtcc cattctggag
     6241 gtctagagtg gaccaaggaa taataatcat gcctctcgac aggctaggcc gatcgcaaca
     6301 gcatattgag aaataggaag ctattggagc aattcactta tgccctagag aattgttgcg
     6361 gatgtggtta ttacgtgtca atgaagtgtc tttccgcacg gactaaaatc gtgcagtttt
     6421 gatgttggaa ggcctcgatc aaaagccctg aattcttcag ggatatatta ttgagggagg
     6481 gacattgcac atttgactat aagttgagaa cctgaaaatt tgagagttcg atttacaaag
     6541 caaaatctag ttcgctaatc acgctccagt gctgtatgca atgacgttat taatttttca
     6601 aacctagaat ctatcatcaa gtatccttgc tctagcaaaa gcttagtgtt cccaagcatc
     6661 tccttaagaa atttccgaaa atttcacagg tatcactttc aatcgctctc gcggatctat
     6721 cctcctccta gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnncggat ctatcctcct cctagtactc cgcaacaaat caaaaaaaga
     6961 ttaagagcta ccttgaaaaa tacaaggata tattactaat accggcgtgg ttaccaacat
     7021 catcatctga attgatactt ttagaagaat tgatggaaca tatcaaagaa tcaccttcca
     7081 ctactgggat actagtcgtc attcaaagaa tttaccaaac atttaaactg agtaggagaa
     7141 attatctgtt tgcaaataac ggttggaaaa catccgaatt gttatctaaa taagaaaaca
     7201 ctatttgaat tatatagtat attatgcaaa taggtataaa acggtaaatt gatttgacca
     7261 taaattacaa atggactgca ttaagtaata caacaattgg aacgttgatg gcttcacttg
     7321 atacaaatat aattattatc gccttaccaa ctattgtatc tgacatccac gtatcacttt
     7381 taacactagt ttggatagtt cttggatact cgcttgtgac tgcatctatt cttcttaacc
     7441 ttggcaggct ttcagacatg ttgggaagag tcaaattata taatattgga tttattatct
     7501 ttacaatagg atcagcatta tgcagccttt ctcaaacagg agaacagctg ctgttattta
     7561 gaatagtcca atcattagga gctgcttttc ttttctcaaa tagtgctgca attattactg
     7621 atgcatttcc agaaaatgaa agaggagaag cacttgggat taatcaaata tcaattgtag
     7681 taggatctgt aatgggactt atatttggag gatttcttac ttcttatctt ggatggaggt
     7741 caatattttg gataaatata cctattggag ttttcgcaac tatttggtct catacaaagt
     7801 tgcgtgaatt aggaacaatt aaaaaagaac aaatagattg gataggcaat gctactttag
     7861 ctataggatt gtcacttatt ttagtaggaa taacatttgg ttcttttcaa attcttaata
     7921 acataccatt tggaatgtat ttgtttgtta tagtaggact atctctaata ggattgtttg
     7981 tatttgtaga aagaagtgta attaaaccga tgatcgatct atcattattt agaataaggt
     8041 cattcctagg cgggaatatt gcaatatttt ttaatgcaat agcaaggggc gcctttatct
     8101 taatcatgac attttatctt caaggaccca ctatgaagct tagtccgctc gatgcaggca
     8161 tttatcttct tccagtttcc atagcattat ctattttcgg accgtttagt ggttggatat
     8221 ccgatagata tggatctaga gtaatatcat ctattggcct ttttatatct gcaattggat
     8281 tctttatgct tacaagtcta ggtactaaat ctactttcat agaaactttg ttacctttag
     8341 caatgattgg cgcaggaatg ggtatttttg cttcaccaaa cagagcatca ataatgaact
     8401 cagttccacg atatcaaagg ggggtagcag caggcactag cactatgttc gtacttagtg
     8461 gaagtacatt aagtgtaggc ttggcatttt tgataatgtc ccatactatg ccattaaatt
     8521 atattgaatc tattttttta ggaacaacgg agataacgac caaagacagt ataaaaagca
     8581 acaacaacag caacaacaat aataacacaa ataatcagaa ccaagttcaa aatggcaata
     8641 ataacaatgt caagatcact atgacactac ttcattctac cacaataatc aataattttc
     8701 taagctcatt acactacgtc ttccttcttt cagctatgct catgctcatc tcaataatcc
     8761 cagtatgcat aagtgctggg aataaacgtg ataaccacaa cattaagaac aattaaagtc
     8821 gttataccaa atttagataa tatattgcat tactccgaat ggctccaatt taatctataa
     8881 ctaatcgtaa ataaggtgaa taattttaaa aaacatttcg ttatatacaa aagcataaat
     8941 atttacgtga acatattatg gcagagacat atggtctact actgctgtga ttccattcgc
     9001 agattaagta aaatgaatta cattaactgc gataccaaag gccaagctgt agtatatatt
     9061 caaaatgttc attttaaaac aaatcttcca tctgacttga gagattatag gtattgcata
     9121 cattgtggta ttgatacaag agtgcatcca ataatcaatt caatggatgt taatcattgt
     9181 actgccctag gagacggcct gcttaataag aaaatagttt tgtcttttgg acacaaagag
     9241 aaatctggaa aaagtgacga aagccatagt aattatcaag attattatgt tttcaatctc
     9301 aattcgttag atgaactaaa tagaattcaa aaagatgatg cagattatcc tattgaaact
     9361 tctatagact attgcccata ttgcggttat ggctattcta ttaacagtga aattcaaaat
     9421 accaattttc ttattcgatc gaattattta gtataataat tcttcattgg aatagcccag
     9481 taagtagcta ttagtattta ctcaatgtaa cattgatttt tgaattttgt ttttagattt
     9541 cgaatttctt tttttgatct ctttatcctg tcaattaatt ctttacgttc tcaacagtga
     9601 ctactaggat tttttggcaa tactaaatct caatggaaat acacagttca agcagaatat
     9661 gaagacaagc actctagatt gaacgaggaa cccaaggccg taaatatggg gctataatca
     9721 atttagggat tggacatgca ttaatcaata acaatatatt ctagtgtcga atatgtggtt
     9781 cagtatcaaa atttagcaac actactctcc cgcgtcgtgt aattgatccc cttagtttgt
     9841 gtttggcgtg cttctacctc tacttctact tgtgcgtcat caatattact aacatctatt
     9901 tttggctttc ctaacttctt acaatacttc agtaacataa ttcttcatag caaaacaaca
     9961 acaacaaatg attagttcag ctttgtaact tattttcatt caagtcattg aggactctag
    10021 tttcgtattg tttggctata aaactgttat tggtatcacg ctgggatatg tgttagaata
    10081 tggtttatct gcttttatca cattcgcgac aatacttggg cgatccaact actcagaaca
    10141 aatgtgtagt tgttgctcgg ttctggagca ggattctaaa ggttttgttg taacattact
    10201 gaaaactttc aaaaacttta tgataggagt taagaaaatg gctcaacgtt acaggcaacc
    10261 taatctaaag aggatattgt ggacaagtat tgttatttta gatactgggg aaaccgcatg
    10321 tatattgact gctgaaacta tagatcttat cttttacaga cattctcttt acatttccat
    10381 acctctgtct ttacttgcag gtatggtatt gtgctacaag ctaatcaaca ctcaaaaatc
    10441 agcatatttc caccttccga cttgcggtgc ttgaccactg attatgtgaa gataaaaact
    10501 gatcaaatat ctgctggttg gtaggaagta cctactaatc tggttagtat tcttgtgcta
    10561 tcaactgcat atgtaatgta gtgtaatgta acgttatcaa aatgattatt gttaaatttg
    10621 aatctgaatc agcagcggag acttttcttg gaaacaatgt tgaactcacg attataggct
    10681 gttcgacagt ttagctctta aatctcaatc agatttaagg atggagtgac tagatacagc
    10741 ataggttcac ctcatatccc tatcttcaag gtgggttaaa acacattttg cctattgcat
    10801 cagtacagcc ttggtgagta tgagcaccat taattgctac accgccgccc tgtccgtttc
    10861 cattaccctt acctgaagta ccgtcggcaa cactaccaac cccgattgca atgccgccat
    10921 ttcctgcatt accattgcca ttagtactac cagatgctcc actagcagta ccaccgttta
    10981 ttgcaatacc accgtttcct ccattttggc ttcctgcaca gtttcatcca atagcaacag
    11041 aaccagatgc tattgccgta ccacctacgc ctccgttggc accacaaaat ccgttagctg
    11101 cgtttccgtc accaccgttt ccgccattag cctttgcact ctcaacctgt acaaatcccc
    11161 catttcctcc gttaccgcaa tgcgatcctg caatactgtg atccttattg ggcaaagcta
    11221 ctatgtacag agtaggagaa tcaattcatc caagaaaccg atattttcag tgccataaag
    11281 gtagtttgta gggtataatg cgtttgctgc tgatgaccaa aactcaagct gccgttctta
    11341 cttctgtaat ccattcgcat gtgaccccaa atttcgcatg gtgttttttg accgcttcga
    11401 gtgtgggtgc gtctaataga caatatacgc ggtcaatttc agggttaaat aaggtgttct
    11461 cgtgtaagag gccatcctca tctctcggag atttctgaag tgtcatcaaa gattcctcag
    11521 tgaatccctt cataggatgt acgtcaagaa atttcggcat gaattttata tcactaagca
    11581 aaaaaatata agaatggcta tttagttatc gcctgattct tcaccttgaa agtcgtgttg
    11641 gctgcctcca ccactatctc caccactatc tccatatgat gcgaatgcat aggtttgaag
    11701 gtgataaaga tacagaagag actatcgtgt ctaatatgaa gatggagttg catatgatgg
    11761 ttttccattc ttctgagact ttgtttttaa atatttttag tatcgttaag atgcctttat
    11821 ttccatgggt ttggatttcg cgttgtgatc caaatacact tcataccctt attgacacag
    11881 tagccaggcg aggactggca ggtcttcaaa gttcgcctga ggtgtggctt cgcaataatc
    11941 tttttccaag tgtaccacaa gagcatcaga actacggtag atcaaaaaag tcaactgatt
    12001 ttgcgatcac gtacttacag atattggcta aggaatattt agatcataat gaatataatt
    12061 cgtataaaca tggcttgaga agttttactg gaaagggaag atggcaagcg attaatgaac
    12121 taactggaga gaaagttctt gatatgcaaa gtgatatcat agaatatttg gaatttgaaa
    12181 aaaatgataa aaatggaatt ccttataaag aaaaaggaaa gccatatgtt ctagtaaaaa
    12241 tgacaacaaa agggtttgat tatattcgcg atctcagaat tataaacatt aataccgtca
    12301 tgttatataa cttattttat gctaaaaagg aatcaatacg aactgcctca actaattcta
    12361 gaaccatcgg atattattta tttgacgatt ttactacaga cgatatattt agcgcatctt
    12421 cgcaagcaaa tggaaatgcg atattcaaaa agaagaagaa gtttagaggt tcttagatt
//
LOCUS       DAOR01000230            4047 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_1916, whole genome shotgun sequence.
ACCESSION   DAOR01000230 DAOR01000000
VERSION     DAOR01000230.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_1916"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1201 a          769 c          712 g         1365 t
ORIGIN      
        1 atgtgtgtaa gtacaacaat taatagagat aaaaatgctg tgtatctact tttgtatttt
       61 tgtaatatat tcattcaaga ttggtataac gagattattc ataaattcaa tccgctgctt
      121 gtgaaagaag cggcaaagtg aaagctgcta taaccgctcc aaagataatc attatctact
      181 ggttaatcat atgaatatgt gaatagcaat agcatatcct atttcggtta tttgaaagac
      241 aatgaggaat gacaaaggtt ctgtttgtgt gtgtggtcac agtcaaagcc accactggag
      301 cgcttcggca ctttacgggt accgtacttc atgctccgta gtgaaatgca actgtctttc
      361 ttttagactc tcgaacgaag gaaaagccta aacgactcag gaaatccagt ttcaatgcag
      421 ttcgctgttt gtcgatctaa atggtacgag agacagaaag gataatcgac atgagtttta
      481 gcttatagct ttaaaaatta tgaacttaga tacgagaagt taataaaaaa tagattctac
      541 tgaacgcctg gtcaagagac tccgcagtac tattaagctt aattatattc ccaaacgtag
      601 ctacattatc tatgcttgct ctgaagaatc tgtccccata accagtcaaa tttttgcgcc
      661 aattacggaa atcaaaaact ttctttagct atttgcctga tgttcagagc gatctacccg
      721 atgattcgaa taagtcaaat aaccaacggt aacaataaga gctcttttga gtctattatt
      781 ctacttactg aatcttctac tggtttcatt cataagataa aaatcacaga atatttttat
      841 agtacaaaag tactcagtgt gtaatgtctt ttgctgaatt aatttaccct tcgccagaag
      901 atactaatct acatgtcact acagcaatgg atagtaatga aaaaacggaa gcgtctccta
      961 ttgctaggat atccaaaaag atagatttca tattatgtaa ttcatgcttt tggtgtgcat
     1021 catatcttaa cttgagtagt tttggcgtta ttgaatgtcc ttcctgtaaa gaaaatacta
     1081 tagaacggat gccactttct gcaaacgacg tttacttatt tgattataat cgagtaaccg
     1141 gagtgattct cgaattctca aactacaata aaggcactat agtatcttat aaatagacaa
     1201 cattaacagg ccactcgatg atgtggcttc ttactaatgc tgaatatgtc ttcttttcaa
     1261 ccaataaagt tcgtgtgaga tcgttgtaat aatgaagtta caatacataa cgaagaaagc
     1321 agttcccaaa taatgttatt aacaactgat gtcacgatgt tcagcacgta tgttgattag
     1381 tggctttcag aaaactggct gaacatatta ctctattgaa gagggtatac tcgttcttgc
     1441 cagcagtcca aacagtaatc accattttct tgaaatatct ctgctggcat atttttacac
     1501 tgatgacata aggtagtatt accatccccg cttgcaacca taatattctg tcaactaacc
     1561 aaggggctat aaaagttcag atttcgtatg ttttgaccta gtgctatgaa tagttgagca
     1621 gtgctatgaa atcctatcga tctcagtaat gtccgaaaca tgccagacat tttgtctacc
     1681 tcctatttcc tgaatatatc atttgtaata gtaattagag aaatattgct acctgaacat
     1741 ataggacaag tgcgaaagat agccttctca ctgtgttgtt ctggagattt gaaaacggtc
     1801 gactccaaaa acatgtttcg caaattgcaa actgacagtc aatatcattg tacatccagc
     1861 tttcagggcc aggtttccca ttcgtcccaa gccccaatta ccttctatga agctgagcat
     1921 agatgaccgg tcggttagac tatgatcagc gatgtttgtc tttccctatg gagaaactat
     1981 cttgatttaa catcaatgtt gaagaattat agtttataat ggctctgggt tacgctttga
     2041 attaggcttt ttgtattgtc tgaatatgga ttgaagttgg acataatatg gaaaaataga
     2101 tttaatactt aatagaattg gaatagtgtg aacaattttt atcttctcag taaaggatca
     2161 gcaatagtac catctccttg tctactgatt tctgcaatgt ctacctcata ttccttacct
     2221 acttcgattg gttgtggagc aggagaacct ctgttgtggc tgcagcgaat cgttaggctt
     2281 ctggtatgtg cctactttgg ctggcactaa tagtccaggt aaacgaaatt aataatcaag
     2341 aaatcaataa taagtcaagt gtcatagcaa atgtcatttt aggaccatct tccaaactgc
     2401 ttgtcaaggt attcggatac ttggtctttc agttcttcaa tgtactgata gaacctattt
     2461 gcacatacat ctgactttat ctgctggttg actcttcttt catttggatt catataaggt
     2521 gcctttttcg ataggtggat tatagtcagc caatctttct gtgcattgac ataatccctt
     2581 accatatggt ttacatggta tgaagcatta tcccatatga gtatcaatct tttccaccaa
     2641 ccacttttca taatatttat cttgagtttc tcaaggcggt tgatagtatt ggtagaattc
     2701 attttatcat ggaaatcata cgccattaat ttctcagctg gccataccat ggatatgtaa
     2761 gcagagaact tctttctatt acctgtatgt gctatctttt gctgctcatc ttcaaaactc
     2821 atggatttgc gaatatgggg cttctgtgtt attgtagttt catcttcaaa agctactaca
     2881 actctctttt tttaagagca gaagccacat gcttgtagtt ttccacttgc tcttttccct
     2941 gttcatagtc atttccatgt tgcagttcca gtctaggtct tttgcatcga attcccaaat
     3001 ctttagctat acgctgtagg tgtttgaaac tgattgaaat accaatctca tttgtaagac
     3061 attttgcaag agaccttaag ctccatgtgt tcctgaggta accaaaaata tgtggatcat
     3121 tgtataatac tgaaaggata atctctctgt cttcgctggt tatgctggat ttcaaaccac
     3181 caggcttctt caactttact tcatcaatgc caccatggat atacgcagca acccatcctt
     3241 gtacagacct gctactgact ccatgttcat ttgctatgtc ttcataagtt ctattcttta
     3301 ctttttggaa tatagcaagt aacctacgat attgcttttt gttatgtgta gtcttcatga
     3361 agaacttcaa actgttccgt tgtcctatta caagtctgag tttggaagaa tgattcacag
     3421 aattagtata tggccttatt ccaagctata actatttcga aatctgggta atgtaataaa
     3481 caataacgac ataaatttgg acacttcacc agctatgagt tttaagatgt aagactagat
     3541 ttcttactct ggttattacg attataggct tcccggtatt ttacatcctt gttaaggtat
     3601 catcgcaacg tggacattca atttcaagtt ctaattctgt cgtgctggcg tttctccatt
     3661 tcttgatatt gtaaaacacg tgcagcattg atctgagtta gagatccttt gcttttatct
     3721 gaaatttatt tattgtttcc gaatgcaaga tgttccccat cgtgacacac tggacaatct
     3781 ttgtctttcc ttcgttccaa gtcaaaaacg actccgcaat ttctgcaaat ccgtatattt
     3841 tttgccatgc taacagaatg ttgttgcttg cttaagtaca catctcaatc ataatatact
     3901 tgaatcggag cgtcatataa aatgccattt ataagagccg acaggtctat gagagctaat
     3961 aaaaaaaggt gacccttttc tttaagtata aacggttttc cttttgctat ggataattgg
     4021 ccaccttcta gctagcaagt aacgcaa
//
LOCUS       DAOR01000231            2872 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_83001, whole genome shotgun sequence.
ACCESSION   DAOR01000231 DAOR01000000
VERSION     DAOR01000231.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_83001"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          799 a          529 c          576 g          968 t
ORIGIN      
        1 caatattgtt tagttgtttt attagttatt tatttggcta gctattgtgt tagaagttgc
       61 agactcaaag ctggatctgt aaacccaaat atattaaaag caccgggcca gcaacttgtg
      121 ctgggaatat gagagcaatt tttctagtag tatcttttct gtttgtgttt taattctgga
      181 gatagattca taatcgttac tgtgcagaat tcctgtgagg aaaaatgagt acgcctagag
      241 tagataataa cgagaaaccc gaaaaagaaa agaagagatt gaaagcgaaa gaagaaacag
      301 aggaagatgc agaagaagct cttgaagttg aatccacagc aggaggcata ggtggaagcg
      361 ggggatagcg aagttgacag actataggga attgtaagaa agacgtgcta aaaacatggg
      421 agctacctac agagcgttgg aggttactag agtgatagtt attgacgaaa aaaccgctta
      481 attagacgga tacaatacaa atttgcaacg cgtgctaatc tataataaca tggtcagata
      541 aagggattgt aaaccctttg tggtgcttat tgattcgatt tcctagtaaa tcttttaata
      601 caatttaaag tcttgccttg cccactcata tagtttactg gccattaacc tattttgatg
      661 ttagagcatt ctcggtatcc ttctctcctt gttttattaa tttttttata gtgctaatcg
      721 aaaaatcagc atcctcaaaa agaaaatgta catcttctgt tcgctcgatt tgtgttatct
      781 ttttaataat cgcacccctg ttatctgcta atttatggta ctctttctcc atttctctga
      841 acgattcttt catcttttcg tctagttgta cattgcttat aatatcatgc atttgcctca
      901 aaagtgttag gtatcttgag attacctttg acatccttat gttattatct gttttatctg
      961 tgtgcattat gtctctggct ctgtgccaag aatctaacat gttttctgga agttcttttt
     1021 ggatacgggg aaataagtta acgatataca cgtctttgtc gtttttaggt gatgcgtcta
     1081 ttacctctct tagtgatgtg ttgctctgca aacttccatc ccaaaggtac ctgccatctt
     1141 tttcagtcca tgctatccca taaaatggaa aaccagcgca cccgatcaca tgatctgtat
     1201 caatattcat aaacttgcta tcaaaagtaa cagatgcgct tttttgtata tctgtagcgg
     1261 ttatgatcag tctcggtata ttggctggat tatttagctt cgcaaaatca atgtatttcg
     1321 ataacgtgtt ctttagcggt gtgatagaat aaagatatgg agatctatag taagaaaaga
     1381 ggccttcatt gggatttggc atcataaacc ataatggcat gaatgtgttc atattaccat
     1441 aagtagctgc atgcattgac gataccatac acctcagacc atcaggcaat actgaaggtg
     1501 ttatcttctc tgcaacgttc agccaaaaat cctccaactg tgctgcaggt tcaacgtcat
     1561 tctttgaacc tgctattatt gacgcattaa cggccccaat agaagtacct gctataatgt
     1621 caaattttat ccctcgttta gccagggttt tatatacccc acattcgtat gcgcccaaag
     1681 atccgccacc ctgcattacg agaactgtct cagattgtaa tttttgagcg tctctgattt
     1741 ttaattgatc cattgtataa ctctcatata ttactgccca aaactacatt agcttatctg
     1801 cgaatttcaa aattgatact gcattgcttg cattgtatga accacactac cttatttttg
     1861 aactcacgat attgattgga aatgattggt gagactgttt caaaaaaaaa tgagatctta
     1921 ttttttattt cgctgtcctt gatcttagta tttgggacct gttgtgggtg gtgtagatgc
     1981 tgtactttta ggcattgccg aagatgaagt cgtactatta ctgactgtcg gatttccacc
     2041 agcagtagta ccttgtacct gtattattcc tttcatagtc gttggatggt ataggcaatg
     2101 atatccaaat gttccagcca tgtttggctt aaatgatacc tgacctgaac taccaggtgc
     2161 tatgtctcca cttgttgcaa gttgtttacc gttttggtct acaattagtt ggtgtttagt
     2221 gtctgttgga ttttgaattt ttatagtttg attagtgttt gctttgaggt tcagggtggg
     2281 attaattgct ccattgctag tatttatcca cgtataactt cccatcatcc ccttggctga
     2341 caattgaatt gcacccgggg ttgctgtgga tgttgtcgtg gccatcgctg ttgaagtatt
     2401 gtttcctttg ggagcagcta ctgccatttg cacatggtat ccatacgagc taaacaccgt
     2461 gccgaacagc atcgcggctg atagtaccga tgcaaatacg attcgtcgaa ttagtctatc
     2521 catgagtcgg ggtatgattt agttgtgatt ataagccttg ctacgaatac cagatgtggg
     2581 ttccacaaat gggattcgta tctatgttct taaggttaac gaaattatta ctccttaatg
     2641 gatgataata aagtagctag tagtaattgc atatccacga aaaaaaccat tgttacattg
     2701 gttgtttttg gaatgataat tgccttcccg cttgttgtta cagacaagct tgcttttgct
     2761 attggcacga acagtacgac aagtggaaca gcaggagcct caataatgaa actgactaca
     2821 acaaatgcgc ctgtagttct tccattattt aaggcattgt atgatagcaa ag
//
LOCUS       DAOR01000232            3723 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_43863, whole genome shotgun sequence.
ACCESSION   DAOR01000232 DAOR01000000
VERSION     DAOR01000232.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_43863"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1205 a          715 c          659 g         1144 t
ORIGIN      
        1 caaccatgtc tggaagtaca acacttaatt ccacagtagg cgaaataatc gttgcgttgg
       61 gccccaactt tgaagcgaac tcaggtgccc cctttacaat tggaacgtcc ttgccatcgt
      121 atttgaaaat tgtgctaaaa agcatgtatc ggttacctga tgcatcatgc agcaaagcag
      181 taaaatacca ccactcctgt gacgttgtcg tctgggctcg atgcggttgc cattctgcct
      241 tcgccgaacg gtgggtcatg ttttgagtat cataactaag ctcagcagtt gaagtattat
      301 gaacattaga gggtttgtga acggctgcag ttgctatagt ctgactgtgt tgtgctaggg
      361 atgtattgtt agcgcctatt gttaataata tagatacaga aagtattgtg gcaaatatgg
      421 agacgtgata actagtaatc atcagaattt gcaactcaca aattccgagc ataatgtcaa
      481 cccaaattta tgcataaatc atgaagtagt tagtatgtat aaaaggatat tcgaaaatta
      541 tactgaattt tttactatat cttaattaat cttctgaaac ataaacctaa aagcttcgaa
      601 tcttcctatt ctgtgcatta aaatcatgga atacaatatc tgtaataaga gccacttcaa
      661 tcttgtttct cgattaagaa actcttattt tcacgcaaag attaacagaa ttttcttata
      721 ttttatcgat ccctgataga gattctaaat tccttaatat tccgataagg agtaacgtac
      781 aatgtataaa atcagcaagt taagtcttat ggggatatta gcaatagcta tagccactat
      841 gatgattccc ggtgcacctg cggcatttgc atctaatcat caccaccatc atcacctccg
      901 ctacaaccac gtgaaaatcc atcaggagat ttcgcaagca aatgcctgtg ataaaggagc
      961 ctcatgtcat aacacagcac aaaacaacgc ttcagttgat ctgccacacc atagctcgaa
     1021 taatgtccat attagtcaac agattgacca agcaaacctt tgcagcggcc aagggacaag
     1081 ctgtagtaac gcagcacaaa ataatgctag tgtaagctaa gaaatggaaa actacttaca
     1141 cccttctttt ttcttcgtgt tgacataatg atcgattgct acttttggcc tgacgagaat
     1201 ggtaagaagt atagacaatg atatacgaaa actggggagt aaatttgtca gtgcttatcc
     1261 cctgtcttgt gattttgtct gtatcattga tgtttttttc gaccccagcc atttctacaa
     1321 tatatactgc tcaagtacaa caagcaatcg cagagactag taatgcaact actacaaaac
     1381 catccagtgg taattcattc ctatcatacg aaaataattc tacactagga ataaaaatac
     1441 agtatccaac taattggcaa aggatagagt ctgaagataa tgttcatggt gtcttgttcc
     1501 tctcaccgtc agaaagcaat tcagacagat ttttggaaag ttttagcgta tctagcagaa
     1561 gcctttttaa cagtaataat aataatgtag atgaattagc tagacgagcg ataagtgagc
     1621 acattgaaca tttgtcagat tttcagctcg tttatacaaa actaattaca gttaaagata
     1681 accccgccta tatgttggtg tataagtata cagatctggt gtttggaaaa gcgatggcaa
     1741 tggatattgg aatgacaaat ggtgataagg tatatgtcct ttcatatctt gcagagccag
     1801 caaaattctt gttttacctg ccaacaatac aaaagatgat tgattcattt gagataccga
     1861 acattgtaaa tcgcttcgtt gaacgctctg tagcacctgc tgcatccatg tgttctgaca
     1921 atcttgaagc aatcattaac cttgattgtc tttccataat gttccaaaga taagcggttc
     1981 ctggatagga tctgtgggta aggcagcaca tacaccgata tcactaaagg gctctgtcaa
     2041 gtatttgtcg tcagtttaaa gtttcgctaa caattatgct taatgcttat aagaacaaga
     2101 attttacaat aattcaattg gataatagcc tagtcgaaga cccaaaggaa aaaagggcgc
     2161 gttttctttt agaattcgaa aacccattta aatgcgaaaa atgtggagaa cgatttaaaa
     2221 agaaaaagca gttgcgagaa cacaaggaag aagtccattc atactaaacg cacgaggacc
     2281 gactttaagg taagtatctc tctctgccga cgatttataa tgaatgcttc tttacatctt
     2341 ggacagtatc aataacaact aacgagttaa aatataatcg actttattcg ttttatcaat
     2401 aattcatttt cgatcggctt ccgaatgatg caattttctt caaagttatg atatgcttgc
     2461 ttcctaaact cataatgggt atcttcagtg gccgtcaaga aacaaatttt aactttgata
     2521 tcaaatttcc taatttcatt gtataaacca aacccgtgca ttcctggcat ttttacgtca
     2581 atcagagcca aatcatatag accactttta aaatttcgta atgctgccag aggatccgta
     2641 aatgaatcga ctttgaaacc actctcttcg agaactactt ttagagttaa attagtgtca
     2701 tattcatcat caaccacaag aatccttttt tctcctgtaa tattcttaac tagttgttgt
     2761 tgtagatttt gctctctgta gaagacataa cctttacttg tatttatgac agtaatggtt
     2821 tcatcatact tcctacaggc cataaaagaa gcgacactcc cggctaaata atcctcgtta
     2881 agacttgagg ttagatttgt gtatcctcct ttccagcaca tatagtaaat aatcctcgcc
     2941 tagatttact acttttataa agatggttta tgatttttca ataaagcagc gtatatttat
     3001 cgtatgttta cgtctattag ttaatagcga atcattctaa aaacaacatt ctataaacgt
     3061 cttctttttt caattcttcc tgctgctccc ctttcgtaga tgggcaaaat agcaactgat
     3121 agagctgcaa tgttgttatc aaagtaaaaa ggattaactc gaatatcaag atcatacaca
     3181 tttctgaagc tgaatgtaac atccctttta ctcacattcc cagtccttgc taatgttatt
     3241 gacaggatgg aggttaccgc cgtatcggtt aaattctaga tataccggat catagagatg
     3301 aaatgaatac caaaaaagat cctcctgcct ttccaccgat ttcaaataat gactttacct
     3361 ctgcagtaag agatgaaata ctaaatagag tcgcaagaga ctacgaagaa ttcaaaaaaa
     3421 cacacacttt tagttttcca acctggctat acagccctcc caaaagtaaa ctattcaaag
     3481 tagaagttga agattgtcca cgttttgggg ataaggcttt tgttgaattt gattctggaa
     3541 ggactgcgtt tttgagcatt gacatgcaaa ttgatttctg tggtccaaag ggctatgttg
     3601 atgttatggg ttacgatctt ggtttaacct cagctcctat aaagtcaatc atgtctgtgt
     3661 tacacacaat aagaaacgga actgacatta aagtaataca tactagagaa ggacatctac
     3721 cag
//
LOCUS       DAOR01000233            2540 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_69342, whole genome shotgun sequence.
ACCESSION   DAOR01000233 DAOR01000000
VERSION     DAOR01000233.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_69342"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    970..1281
                     /estimated_length=312
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1891..2171
                     /estimated_length=281
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          644 a          347 c          365 g          591 t
ORIGIN      
        1 attgtaattt cgattccatt catggtcacc atatctgcgc ttttcgtgca accattcctc
       61 aatgacgttg aacttgcaga tctgagcttt tatacgaaaa tatttgatat gctaattctt
      121 ggctacgttt tcaagagtac cgttgtatct ttggtaaagg gagccaatat taaattactc
      181 tatgttccag ctgcatttgc attactttgg cttggacagt attcattgat gattaactat
      241 tttgataatg attcaattcc atttatcgga tcaatagttg cacgactcgc aggactagcc
      301 ttgtttgtct atgctataca ttatgcaatg tctggtggag tgaggaggga aatggagatt
      361 gaagcaagaa aaaagacata aactccagct ccattatgag atactttgcg ccatagaaga
      421 gaacatgatg aataatgacg ctgtaagacc tacccgcata caacatagtt caagattgtc
      481 ctacgataag atgatgaacc atttcgatga gttagaagga aaggggatga tatatagaga
      541 cagaggactg atctcaataa ctagtaaggg aagggaattt acaaagcaat ataatgacct
      601 tgtaaatcta attgaaagag ttggtctgta ggagataaca agtatgacca tcatgatagt
      661 ctagttatta ttcatgcaat tttgctggga atgaagatga caaaatgata gcggcaaaaa
      721 aagaaaaata gtagtcgtga tttatttatg aattactctc ctgaagagag tcctacaagt
      781 gagcaaatga taaaggaaag ctttactagt tctttatgat cttatcgtag tttgactctt
      841 aacaaatcaa tgtgtgaaaa tacccttaac aatgtgaagc gtttcaatga tacaaaataa
      901 actaaaaatc ctggcttctg tagtaacgtt tgttgttatt gtcattgttc tatttgaatc
      961 agtagttgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn naagttatac agttagaagt acgtacattg aaattccagg
     1321 ctgatgcttc agcagcatct aatgacttgc aagaagtaca cacaaccata gcattgaact
     1381 atcacattag cccagatcag gcaaacatta tctatcaaca gctaggagct gactattcaa
     1441 ataagataat agcaacaact atacaggaat cggttaaagc aagcgttgca aagttcaatg
     1501 ctgaggaact aataaccaaa agagctactg caaaagctgt aatagctgag gctatagcct
     1561 atacgctatc agcaagggat attgttgttg aaacggtttt tattactgac tttaaattct
     1621 ctcaggcgtt cgcaaatcag gttgaggcaa aagttgtggc atttcaaagt tttctgacag
     1681 agcagaataa actgaaagcc atacaagtag ttgccaacca aacagttgtt caggctttgg
     1741 ccgcagcaag atcaaatgta gctaaagcca ccggtgaatc acaagcaatt cagattatta
     1801 ctgaacaatt gaaacaaagc ccagaatacc ttaaatggct aactatcaat aggtggaatg
     1861 gaataatgcc ttatgctctt ggtagtggtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn ncgtgtattt tccagttgct tgatatgtta ggaaactcta gactacacat
     2221 catccctacg atcccacttt gtaatgttgt ttctagaata atgactaagg taaagtaacg
     2281 tgtttttcgt tcttatatta tgatactcta tagactgttc atcgtcactg ctagtatact
     2341 ctttcttaac ctctttgata acctgctgca gatactctcc attacaacaa accctagctt
     2401 tttactacta ttagtaatac accacaaacc atgaaatctc cttattggga ataaagaaac
     2461 agagcgttaa cccaattctc acgtatggta tcctaatatt gattctccta gcctcatatt
     2521 cctgttacat taatactatg
//
LOCUS       DAOR01000234            5059 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20876, whole genome shotgun sequence.
ACCESSION   DAOR01000234 DAOR01000000
VERSION     DAOR01000234.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20876"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    385..575
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3819..3987
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1483 a          855 c          896 g         1465 t
ORIGIN      
        1 cacgaagaaa tgaggtcggc aaaaatgatt gacgatgcac taagagtttt caagacagaa
       61 gagtattctc ggtttcaaac ttttgatgat gcggttgatg aactaacatg caagctatac
      121 gaaggagaat actacaagag tccaaattca tctatatgtg ttttatataa ccttgaaatt
      181 cataggtttc agacaattgc atcttcttcc atttcctcct ttcaatatag tgatgcgttt
      241 acaagaacag ataatgaaaa tagcaaagca cggtatcctt ctactacgct tactatagta
      301 gaaattaaaa cgaatcaaaa tgaattcgac gactatatag agaatatcat taacaaacac
      361 acttacacat tagaagacct aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaga tgtaaaaaga ttgattagca
      601 agttgcaata tgcgccacaa aaaagcgaca aggtggcact acttcttgaa gaattgcatg
      661 atattcctcc gggtattgcg agactagttg caaggaatat atgcctggat attccaaagg
      721 tcttatgact ttctgaacat tctttatgat gataatggat tattgagccc tgaagtatga
      781 ccgattctaa aatcacccgc aagaaatatc gatgaaaagt agcagctaag attgacacct
      841 ctgacttgtt tttgaaatgt aatacaggtt acatatatct gcactgtgaa atcacagcat
      901 ccttacagaa tgtgcaaacc gcagcggtag gtaagataag cgttacctac tttgcagata
      961 cttactacca ctctttaata tctcatgctg tcttatataa gcaatgaaag ctgttcaaat
     1021 taagaagtat ggaggcagcg aagtggtaga gaccaaccaa agtgcccctg aaccaattgt
     1081 atctgcagga aaggttctag tcatcgtaaa agccgctggc gtaaatcctg tcgattggaa
     1141 aattcgtgaa ggtaacatgc aacaaatgat tcaactccaa ttcccatcaa ccttgggcat
     1201 ggatttttct ggggttatca aacaggttgg agaagggatt tcagctttca agcaaggaga
     1261 cgaagtatac gggcaggctg cggtaattac cggtggctca ggagcgtttg cagagatggc
     1321 tctagcaaat ggagaaacta tcgcacataa gccaaaaagt ctaagtcatg ctgaggccgc
     1381 aggtttaccg gttgtgggcg ttagcgcttg gcaggcccta gtagaaaata tcggactatc
     1441 aaaggatcaa aagattctga ttcatggtgg cgcaggaggg ataggatcta ttgccatcca
     1501 acttgcaaaa cacctaggcg gatatgtagc aacaacagta gacacaaaag ataggcagtt
     1561 tgtccaagaa ttgggtgcga accaaatgat agactacaag gcccagaatt tcgaagatat
     1621 actgcatgac tatgatgcag tctttgatac tgtaggaagc gaaacgtaca caagatctct
     1681 caaagtgtta aagaagggcg gcataattgt ttccatgcta gaacagccaa actcggaact
     1741 aatgaaccaa tatggaatca aagcaatatt tcaattcact caggtgaacg gagaacgatt
     1801 gactaaagtg gcacaatggg tggatcaaaa taatataagg gtaaatgttg agaagacgtt
     1861 ttcgctggat gaagcaggta aggcgcttga ttaccaaaag gatgtacatc caagaggcaa
     1921 aatcgtctta gtggtttcaa agagataagc acaattcaaa ccttcacaac gatattggca
     1981 gtagcacaac aataccatta aaccataatg gaattgtagg aggtatgata aacaaatgaa
     2041 catttatttt ttaatatgcg aatcgtgtta tctagttgcc tcaataattc agaatgataa
     2101 gggagaaaaa atgcccggta aatggttgca aaggtaaaat ctacatatta gaaataatgg
     2161 gcttaaaata atggcctgga taatgagtga atttccttca ctgtctttta atttgaagga
     2221 aagtgtagca ttaagtgata tctcttaatg actctctcta gtttgtgcat acaataataa
     2281 tcttgaagta ttgaatatcg taaacttgat tcgatacagt aatttgtctc acatatttgg
     2341 gaatcattat aggtcataca catgcagatt aactaattaa tttggaatac aatatagatt
     2401 ttagttaacc gataccaaag atggttcaaa ggctgattta tttgaattct cttaacttgg
     2461 cctttccttt ctaaatcatg tagaaccttg tctatagttt gtggattgta aagagattct
     2521 cttactatct ggtcataggt cagcgtctga gtttctatga ttgataataa ttctctctca
     2581 catggtacga atacttgttt gtccatacat ttgcttacaa ataagataat taccagtacc
     2641 ccatactacc ctatcagact cttctagaca gctagaacac gttgtcaagg taagtgagtt
     2701 attccattgc tagtatgaat tctaaatcca ttaataattt ccgaatgatg gttcacaatt
     2761 atctctgaat ggggatggaa atggaaggga aagggcaaag gtaattgaaa gttagagttc
     2821 acattatcat tgaaacattg gtctactatg aataaaggtg gaggatcttt atgagtatcc
     2881 gagatgcatt tgtagaaagc gtttatctga ctatcaagtg tgtggctgcc tgtatgtact
     2941 gtctcttgga ttactccgct atcgctactg gctgaagcca ggcctggtcc tattgtccct
     3001 aacactaata atgttaggat actaggcaca attgactttg tgtgttgaaa cattaccttc
     3061 tttcatggtg agtcgtagat atcagttttc cgttcaccca tactggttcg cattgaggaa
     3121 ttggagattg ttgtggttta aaaaattcct cgatttgata tacaagtagg tgacaggata
     3181 actcccattg ttgttgtact ttgtgcgatg aaaaacaaga gcctccacat tggctttgct
     3241 aatcacaaca tgtgaatttt ggaaaactct ttacaatagg gctgccttct tattgattcg
     3301 gtcagtctac tcatttttaa aatgacgctg aggaggaaaa gtcgattaaa ctacaggcga
     3361 ccgatgttta agaatagaat aatataatga aattcaatgc aaaacagcga aggaaattaa
     3421 agggtgtatt ctggaatcat acgaagacac tatcaccgaa ttactttctt atgaattata
     3481 atcatgataa tgacctatat ccaaatatct ttcctgttat aattcttcaa ttcaaaaatg
     3541 ataatcaaaa acaatcctac ctatactcca tagaaccttc actgcattca taccttcact
     3601 cgagttttaa atgcgttaat gctcacgaaa tacttgtctt tctcaactta tttcattttc
     3661 taagaagtta gattgtgcca gctaccggag gcataaaaaa tttcgatacc ttcgtcattc
     3721 ttattaccat tattattggt gatgtgatta tctaatctct tttgttcctg attcaagaat
     3781 gctgtctctt cccttttctc actcaaagat gattcgtann nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnttg gccttttcaa gttccaagtt atttatttgg
     4021 gtactaaggt attcaacttt atcttgaaga tatggtaatt cgttattgtt agcaagtttc
     4081 agcacgttaa ttatttcttg ttcaccgatc cgttgttctt ccactatctt gtgtaacttt
     4141 agaagtgacg gtaaggaatg tttattttct tcatatacca catttaattt gtgcatacgt
     4201 ttgagttccc aaaattctcg atatattgca tgtacctcgt caggaggaat atctaatgct
     4261 attacaactt gtacaggtgt cttaccctct gaaaataatt tgaaggcttg tgattgtttc
     4321 gacttgtgtt cgttatcatc aatttcctcc tcatcagtat gtcctgcttc tctttctacc
     4381 tctgccttga ccttgtcggt tatgactctt atgtcacgaa acgacatatg tgttcgttcg
     4441 gcaatttctc tgcttgtctt atcctgtttg taaagttcta taacgtattg ttctttttgg
     4501 gccctagtca tatttgataa cgacgtcatt atacataata atacacacac tcgttgatag
     4561 ctttttccct gtttccatct gttacccgta tatctcgact tgctaccgtg gtgtttccat
     4621 ctgttttcag ctgcttctct ctgttaccgg aaggatcgaa ctgtgcggga agtacccagc
     4681 cctatgtact ttagaaaaat aaattaaatc gtagaaaaaa actggcaaaa taggatccat
     4741 tctaatatcc tgaatcacag atccgggcac attatctcaa atcttcaata taacgacaca
     4801 acagatagac ataaggtata ttagaggtcc ttcacaaggc ctgctataaa tataagagaa
     4861 ttagtcgaca tattggacca ctaatatatt cataagcctg gttgtgcgtt agatatagaa
     4921 tctataataa agacgctaac tttttttcct tgctaccaaa cttttggatg gtagtcatta
     4981 ttgttttacc tctaggattt ctctacaaaa cgtacgaacg tttagtctgt ctaggcagga
     5041 gcatcaaact taaataatt
//
LOCUS       DAOR01000235            2637 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_84092, whole genome shotgun sequence.
ACCESSION   DAOR01000235 DAOR01000000
VERSION     DAOR01000235.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_84092"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          753 a          520 c          490 g          872 t
ORIGIN      
        1 ttagattagt ttagttctta gttattagtg cctttttaga ttctgtttct tcaaatcaga
       61 tctctccatt ttcatatgaa atattcactg ctttctccat ttccatgata catgaattac
      121 ataaaaatac aagggtggta aactgcgaga tttcttccac cttgattaaa ttgccacaac
      181 tacaaagtac tgcttcttct agattagtca ttttttattt cagctatctt cttccacnna
      241 tatatctgaa gtggcaaatc ctgcctttgc tgatgagttt gtaaaacaac ttgacgaatt
      301 aaaggagtta agaaatgcat tatttgctta tagacttgta cattataatg ataaaatacc
      361 caacataaaa ctaaacatca caaacagaga aaagcagtta ttcaagccac ttttgagggt
      421 attccaaaac acagatacac aatctgagct atgaaaagtc gtcttcctca ggtttatcgt
      481 agacacgttt ggttttgata gtcattctca aattatcgtg ctccttccta tattagtagt
      541 ggaatctatt gctcatgatc tctttatttc agctatcttc ttccacctag gccattttct
      601 ctcaataatg aaatctaaac tatatcttga tggtccgaag gcagcattga tgactagcaa
      661 caaaaaggag acgatcgcat ataccatgcc agttcctatg tctgttgagc ctgggccgta
      721 tggtccgccg aaaccttcag gaacagacca tatgatcaag ctcagaagca agctaactgt
      781 gtatgaaagc ttgcgcagga atccaaagat aataccgaag gcaagagcaa gttcaataag
      841 tcctgtggag tacacataga atgagggatt tgaagacgtt attgaagccc agaacgaaaa
      901 ccatcctgct agccatgatg gctgacctga cgcagcatcc tttattacgg tcgagaaaga
      961 atctacaaat cctggtgcga attttagtgc tccgtctatc gcccagaata tcccaaaaat
     1021 tattcgtacg atagttttta gtgataatag atttctgacg aaccagttat cgacattaag
     1081 ggtcatcgtt ctatcacttc atttgcatct ccgtctgtga tggtaatata ctcaaattat
     1141 tgtactccta ccttgggaag catccttgaa tttgtcaagc atatattcat gaacttttat
     1201 taggacttta catgtcttac aacattcacc tgctcttagc tcgtcggcgg aacacctaca
     1261 acctatatcc ctaagggtgt gatcgattat ccaccaagga ctatttttcc attcaaattt
     1321 gtgatttctt tcaatttgcg cccatgactt aaccccttgc tcttcttccc ttagtttatt
     1381 tctctgtttt tctctctgcc attccttgtt agtcatgttt gctggttttg tggaaggtgt
     1441 aagtttttct attgctccaa aaatggatgc tacgtgtcgt gcatgtcttt cattctggtg
     1501 ttccttgact tgtttaaatg tagactcata ctcaggagtt tcgtcatcaa tcctaatttt
     1561 gtcaacgtaa cgttctattg gagttaatgc tttcttcttc tgttctgaac tttccttatg
     1621 ttcctgtctt tgctctctat cgttgcattc aggacacaga aacttatgat tccttccaac
     1681 gatttgttta ttgcatataa ggcagtttga ctcgtcttta cgcagctcat gatatacgaa
     1741 cttgggcgat aatccttctt tcctccccaa ttctacaagg tcatcctgtt tttgtagttt
     1801 gattctagtc ctctccttca gttcgggtat atggaaataa atacgaccag catcccaaat
     1861 caacttcaag tctttaagtg cagttaagaa ttcttttgac ggttgtttag atttagcggt
     1921 gggagttgtc atttcttatt cagtatttct ttagagtcac agagataata gcatttaatg
     1981 ttgtagaagt cagattcgaa tctagttaat gataaacctg attaaatttt atcacagtag
     2041 tcacatttcg agctttgcgc tgatggtaag gtagtcatat cttacaggtt gtagcttgat
     2101 ggttctgaag tgactctgaa cttatgaact cagcattaca cttgtcgcat cggtagagta
     2161 ctgacgtcat ttcctactct tctccttctc gtaaagacta attgctctct acaatttcct
     2221 cataagtttc cccttttatt ccaagtttac gaagatagag gaaggaagca aaatatggat
     2281 acatacgcac aaaaatgaag gatttgacta caaactgccg ttagacaaat tgaatgaacg
     2341 gcagggggat cttgaactgc ttgatagcgc agagaagata ctgcactata gatgtcatgg
     2401 atatcaggag agatggggat agaaatgacc acagttaagc atacacctgg aatgtcctgt
     2461 aaagtgtgat gagaaatgac ttgtgaaatt aggcgaagaa agaaatattg ctatgaagaa
     2521 ggatggattg agatgacaga agcttactac tttaaggatg aaaaataagc aatgaaagtt
     2581 aagcccttac gatgtcattc atgtggcgct ccagtggctt ggtatgatgt ggacgac
//
LOCUS       DAOR01000236            4762 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_127041, whole genome shotgun sequence.
ACCESSION   DAOR01000236 DAOR01000000
VERSION     DAOR01000236.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_127041"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1915..2080
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1508 a          796 c          949 g         1343 t
ORIGIN      
        1 tatcagttct ggctagagta tagtatttgc caactacgcc aactacggaa ggattattcg
       61 atccttcact atagggatag gcaaagattg gtggattaat tccatggtta agaaggcatt
      121 gcttcgatcc acctatttcg taatttagag aagcctgtga taatgtattt agatgagggt
      181 gagtcatagt atgtgattct atatccattc catctttttg taaagctgat acgtcctgcc
      241 acgtcttttt accatttgac cctataaaat tacaaacttc aaagaaggta gccttaaatc
      301 catatttatc taggattggc ttggcgtata gaaattggtt tttgaaagaa tcgtcaaaat
      361 ttataatgac tactttatct ttattattaa tgctaaggta actattagga ttagagcttc
      421 cgttagtagt cgcaagcact acaccagatg atggtgaaga tttagataga gtagaagtgg
      481 aagatggagg actagcgtga gtacgatggt ggtgactagc acttttagaa gagctagcaa
      541 ccgcctgcag agttggtgtt gttgttttga acttgataaa gcttgttgga cttgctggct
      601 gcggcgaaga tgatgactgg tcactacgag aataagaaaa ttggctattt gtcgtagaag
      661 gtgaaataat taccatgctg ctaacaactg ttatcgttaa gataatggca agaggtatcg
      721 acgacctggt tgcttgaaaa tgctttctgt aattgaaaat caaaagagta attaccatcc
      781 gaagtgacgt gcgaatctac ctataattat tgtcaaatga aaatgcatac aaataattac
      841 atagattgct gaaattagtt tatttgtatc aagcatcaat aattttggca cgaaaagcag
      901 atagtccaca aaaaaatcaa aggtgtttcg actgaaacaa tccttgattg ggaagaggaa
      961 caatattgat gatgatgaca acaataagat tccaattcat cttaggagct aaaccaagaa
     1021 ttagtattga aaattaataa caaaaaatag atggacggaa gtctcattta ctggttcatt
     1081 ccttggttag agtaacctgt gtaggctgtc ttattctcta aactttttct agatatttct
     1141 cgccacactt gtgtaaatag tatccagaga tgtctcctcc gtgatgatcc tttacacagt
     1201 aattgaattg tttgagatct ttgtggtcgt cgtaatcgtg gtctttgtat gctagtgctg
     1261 gagcagcaaa gctgcttact gagacgaggc ttactgctgc tacaatagca aagattgaca
     1321 gtgtagttgt cttcatgtat tttcactacg aaaaccacaa acatcttttt atgaggtggg
     1381 tattatagct acagaattag gttgcgctaa aacgatcttg agtaatcatc caatttttta
     1441 tccatggaca tatttctgcg cttactctct tgaaaaatgc gttgtacctt atttcttgca
     1501 aattactcga ttgtaatgaa gtctagatct gctgctctaa attgttataa gacgcttatc
     1561 aattatgagg agcaaaagaa agaggcaaag acgctaagag gatatactag ttacgcgtca
     1621 atattgaata tgtcattttc gagctggaaa gaccgggtaa gacgaatatt gtatagaaat
     1681 aatgatgcaa aacaaaagac taatgacact ttacaaacga ccgccagacc agcaatatta
     1741 caatcaaaaa caaggtcgga gcttacaatg ggaaatgcta tttacaaaga cggaacaata
     1801 atgctttttc caactaacgc taatggttct cagttcatac tagaaaagca gaatcctaac
     1861 aatctgaaaa gctttacaac tgacagccat tatagatttg tcaagaaaaa agaannnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tatagaattt tggaatgtat
     2101 tttctggaga cataacctat agaagtagtg gcgaacatgc gaagagttgc cgactgaaca
     2161 tgttcgcatc aactacaata caaatttacg actggaaggg tgcaagggat aaaggatata
     2221 tcggaagcga aaaggatttg aaaaatcacg aagtgaccgt tattatgagg gtacacacaa
     2281 aagtgggcca caaagcagaa tgctgtataa aaatgcgtgg tggtggtcat catggagata
     2341 gacccgagca agcagcttgt attcaacttg atgtttcaac tcaagattcg ggaaattccg
     2401 caagatgggc taaagaattg gtccatcctt cgtatgagta tcaagaatta cagccattgg
     2461 ttgaattttt ctttgaggaa ggaaaatggt ttggtatgaa gaccactagt tggaataata
     2521 atgatgataa taataataca ctaaacacca caacaacaaa tagatgctac attgaccctg
     2581 acccctttaa tactaatggt acattgaaaa atgattatcg attgtattca gaacatattg
     2641 accgaggaaa tggtaaaaaa tatcgagaaa tcgtaaattg ggcgggtggg gtgcctacga
     2701 cagtaagagt tgatggtttt gaaagtgttg acttttatgc ttgcaacgca cgagaaatta
     2761 ttccgcctag agattagatg atagtttgtt cgttatgcct agaagtaaag agaaacttcc
     2821 agacatacct tgcggttact gaaggttact aaaggcaatt attgagcgag atctgctcag
     2881 tctgtaataa ttgtaacacc gtaaaaggag caaaaggcaa atttaggatt ggggaccgct
     2941 ataaaaattt aaaaaaccaa tacgttggta ggctaactac aaagaatata tatgaaattg
     3001 attaatttac tctattagat tgctcttgcc gcacagattg accgctctcc cgctattgct
     3061 cgtagcatct ttgatcctat cttctagtgt ctcatacatt tttgctgttg ttattcaaca
     3121 ggcatcggcg tcatcaatac atcataatca ttttcaccct ataactaaca gcaagaataa
     3181 taagaatttc ttttcaatat ctccttctat aaatgtctgt tgtgcttggg ataataaatt
     3241 tgcagcagga cagttaacat acataataag cggtggtgat gcttcatcta aacaagcagt
     3301 tgttgaagca ctgaatgaat gggcttcaaa tgtcaatgga ctccaattta cagaagtttc
     3361 agacaaaaac agtgctaaca taacactgaa ttttcagaat ggaggaagcg gcggcggtgg
     3421 tcacaaccag gtcaatggta taggttccgt caggggtagt gggcataccg acatagtggg
     3481 agaaacaata ctccatggta gtaatggact cataaatagt gcccaaataa ctatagccac
     3541 agctgctttt gggtcttctt ttgggacatc tctggtaaaa caaatagcaa tgcatgagat
     3601 tggtcatgct cttggaatcg gtcatgctaa tttcaaagga gatatcatgg ccccagcgat
     3661 taactatgaa aaaatggcaa tatctagttg tgacgttaat gctgtctcat cggccaatca
     3721 atggaagctt gcgaattcgg gcacagctcc acaaccacca catctggacc atgtaaactg
     3781 ttgatgatag tattaccacg tatcggggag atgttcaaat gtggcactat atcgtgtgta
     3841 ttattcgtat ttgaggcttg ataaattttg tcaagaattg ttgttgttct tccagctgta
     3901 actctaatcc tggctgtctt tttgccaggt atgatactac tagtttcagc agaaccaacg
     3961 gtctatgcat aattcaaagg ctattttaca aaactttatc actaacacta acaacttttt
     4021 tcccgccagc agctttagta gtaatggtgg cgttggtcgc ggatttgcca ccaggatagc
     4081 aatcataaat tttgatgatg gatacaagag tcaattcgca aacgcaaaac ccgtactaga
     4141 taaatatggc tttaaggcaa ctttctttat agtctgcaat ttcgtaggaa aatcagcaaa
     4201 gcaaatgaat tcaacttcta tcgttaactt cgctggaaaa ggagttgaac aaatcaattg
     4261 gactgatgta cttactttat acaaacaaag ggagatgata ggggctcata ccatgaatca
     4321 cctacgaaat atgacgaata tgtccgatag ttgaattacg aaattggtag ctcgaagcaa
     4381 tgtctcgtta accacgggat tccaacaacg accattgcgt acccgtttga gaatggtaaa
     4441 gataccgtaa cagtagtcaa aaaagtctcc caggattaca cttatcctag atcgggaaac
     4501 tatccgctga tgtttctctc tttgcgatca aggttggagc cacgattatg atagaatagc
     4561 ctatttgtat aatgataaac aaaagcataa tagattcatt caagtcgtta gtggccaaga
     4621 caaatacaac agaccagggc aaccgctaga tactattcca ataataatat atcactggat
     4681 cgataacagt ggggttcaat acagtactat gtatctctct tttgctgaag gaatgaatat
     4741 ctacatgaca atggctttaa gg
//
LOCUS       DAOR01000237            2502 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_80920, whole genome shotgun sequence.
ACCESSION   DAOR01000237 DAOR01000000
VERSION     DAOR01000237.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_80920"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          823 a          447 c          470 g          762 t
ORIGIN      
        1 aagtctgtaa gtgaatttgt tacctatgtg tgtaagtaca acaattagct atagtggtgg
       61 tattacaatt ttgataaata atatcaaatc tagtaacatc atgactggaa gcgtcacgac
      121 gccattatct acaaccacta gtggcagcaa cgggacgatg gatccagaaa aatttactgc
      181 aaaactgacc tgtagtggta ctggtttctt ggcttatctt tgctgacaat gttaagaact
      241 aggtaagcaa gataagtgcc gatatctagt ggagaacgga ctgcttactg taaaataatg
      301 gtggaaaagt tggaggagaa cttcagcatt agaggaatat ataacgcgat tggcaaattt
      361 gttctgcata atataattga tcattaaaag aaaaacccag ctccttgctt atctggcttt
      421 tgggttgatg aaaaagtaac atttcgtcac aagcttcaaa atatccattg catgcataca
      481 tccagacaac accaacatgt attacatttg agcttatctt taaccattta gtatctgtag
      541 aatcaatatg gaatgtagct ccgttagcaa ctactagatt tgcatttaga aaccacacac
      601 catttgtaaa ttgcttagca agaatactgc catcatggag tttgttatca atatctgaga
      661 gtccagttgg actactacag ctgacaataa ttgtccttgt ggaaggattg taattgatgc
      721 aactaccagg cgaggcagtg tttgattgag gacgagaaga agaagatgat ggatttgtat
      781 tgcccttttg tgtgatagct tttgccttct gtatagaaaa tggaatgacg ctaaaaccga
      841 tattttgaga tatgaggagt ataagaacaa agctgatagt gacaaaaaca atagctgaca
      901 tagttatccg aatatgtctc ttcacttcct acaagaacaa aatcctattc caaaatttat
      961 atttttgaga ctattgtcgg atactgattt tctttacaat taatttgatt ttttgtacat
     1021 agtctagtag tagaagagca ttctgaagtg aaacaataga gataaaagta cttctttaca
     1081 ataacgatta caccacgcga gtgcctcgga taggcgatag gcctccatag ccagaatatg
     1141 ttctgcagtc tgtctgttga tgattcttcc atccatcaca ctgcagaaaa gtaagcggaa
     1201 aaccgctatc agttctggct agagtatagt tatttagcaa ctgtacctta gattaataaa
     1261 agctacaaaa tggtttagtt gttttcgtcg tcgttagcct atgattatcc aaaaaaagag
     1321 gagtgagcct gttagtgatt ttgaagcatg taacgataat cgtgaaatta tcaaatcgcc
     1381 agtatacacc gttgcatgtt cttgttgatt gagcatgtct gaccaataca aaaacagttc
     1441 gcgaacgcct tatgctcgtt cgcgaattag accgcggata gaacttttga atccagctag
     1501 atgtacttct aacagatgtc cgcgaaatac accgaattcg cacccaatcc atgatattat
     1561 ctcaaaatga tcgcggatcc gttttcggtt cgctgatttt gtaaacagtt gtaaacattt
     1621 gtttacagag gggaaaactt gtatacaaca tgtaaacaca agaaaaatca cttagactta
     1681 tttagtgaaa tttgtaaaac aatctgactg tacagcagtt gatgaaagat ccaaagtggt
     1741 tcataggaag aatacaaata tcttgataga attacagtaa ttttagccag caaacgctaa
     1801 aatcaagaac taaaccgtag aatacttgca aaatctataa catttatcaa atggaaagtc
     1861 tcttcctcta tgtagttgga tactgaattt ctttcttcct ataagacgaa cgtcaaaaaa
     1921 cagcctttaa gttaagtgga aataaaggaa gaacctgtca tccagtagta gtagtcccac
     1981 taacaatcct gcctgaactt ccaggagaga tattttttat gtctagcaca ttatgtgttg
     2041 tatcgtatcc aatctgattt agagtcaata ctttaaatcc gttatcatgt agatatttca
     2101 tttcttcatc aaataaattc acagtcgtat cagcagcgtc tttgctatta gttatatcag
     2161 gataggttac aaaattatga tatatgacta tggaaatagc ctttactatt ccaccttgat
     2221 tgaatttaag ctggctattt acatctgcaa taaatacctg caacatctgt gagttatcgt
     2281 aggaatgaca aacacctgca cacaccgctc cttcttgaga agaagaatat ggaccatcaa
     2341 tatgtcgatg agtccagccc ttaatagagt agcgatttgc aaatgttaat gttcctccat
     2401 taccagaata tgttctgcag tctgtctgtt gatgattctt ccatccatca cactgcagaa
     2461 aagtaagcgg aaaaccgcta tcagttctgg ctagagtata gt
//
LOCUS       DAOR01000238            6908 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20951, whole genome shotgun sequence.
ACCESSION   DAOR01000238 DAOR01000000
VERSION     DAOR01000238.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20951"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2081 a         1321 c         1276 g         2230 t
ORIGIN      
        1 tcgttgcatt ctaggtctac aataaagtgt tgcacaaggt actctttacc tctaaaactc
       61 aatattttct gcttgacttt aattgcatct ttgttcttat tacagtatac atttcctctc
      121 atatcttaca tttctccagc cattaagcct acaatagacc cgctaacccg tttagaatgc
      181 tccagcaagt aggcagattt gaagtgcaat atcgtataac gccattaact atagcgccat
      241 tgttctgtcc tgaattatcg ctactactgc cgtcatgatg atggtcatga tgcgccatcg
      301 ccattgtcga tgtcgagtct aatgctaata tcgcaacagt tgcgattact accatgctga
      361 ctgagaactt ttggtctgtt ctgattgttt tgctttggtt gtattgatgc atacgtacca
      421 ccattacgtt gtaccagctg cctcttcttc caggtagatg aaattaagtc gaaaacaatt
      481 ggagattacg ttgcattttc gttgcaaaaa gtctatttca accttctttt gatcaccaat
      541 aattttgcaa catgtagccg aatcctgcta gccggagaaa ttatcggttg ggcctcaaca
      601 aaatgttatt ttttcttagc aaacataaaa ttttcaccct ttatactaac tatcgctttc
      661 ctacagaatc ttggataaat acatggctgt cagctgctcc agactatttc cctttttcta
      721 ttaaggttaa ccggtacatt actgaccaca cacgattgaa agtagaaaaa gccttacaac
      781 tgtgtacaaa tttagtaaaa cgctttacag gataaatgat tagattgact tctagcttct
      841 taagatgcta ccatcattta attatagttc agaaaatttg aaaacactga gtaacttctt
      901 tagcgaaatt aacttaagta acaaagctgt cctagaattt agagatcagt catggtagaa
      961 agcggtagat aaaattgaag atataggaat tgtttttgtt cagttgatgc tccatcgtct
     1021 gcctcagctt tagatacagg attcacgtac tctaaacata acaatgtcca tcaatgcgac
     1081 ttattgatag tgaattggtc tttgacagag ccatcatttg cataaaaatt cgcaataaat
     1141 attcttccat tatttgtgat ggttacatct agaaatccat gtcccatata ctgactagct
     1201 atgtaaggag cctttgcaaa tagcgggaaa agatgtgcgc ctgcagttcc tacgatagca
     1261 aatatccttc cttgcggatt agtataattg tgaatgttgg tatcggttat tagtgagttg
     1321 gttgaattac tgatgttata tttgatagga tatgaccttt tgtaattgtg ctcgtgtcct
     1381 tgcaacacaa ggtctacatg atatttatca aatagtggat gatatatgtc cctaaatgta
     1441 ataagaggtt gaactaacga tgctgaagaa tatgatgtct ggtgatagta tactataatc
     1501 caatttatac gtggatctga tgatgctttt gcaagatcgc tgtctacgaa gctatactgt
     1561 tcagaaccta ttcctattgc tggtagctct gtagagatcg caacgaagtg aacggcttca
     1621 taattgaagg aatagtattg ctttgtgaga ttaaaatggc ttatgtaaaa attcaaaatg
     1681 gactgagaat catgctcatg attgcctatg gaaattttaa gtctttgctc tataggttta
     1741 actattttaa accagcaatc tggactacgg ctttcattat agtcgctgtg atagtagtca
     1801 cctactccaa gtactaatct aggattcttg cttgctatat tatccacagt agttattgtg
     1861 ttggaattac aggcccaatc tgagacagct ccaaagctaa aattgggtaa tacttttgtt
     1921 aatgttgcag cgtatgaaga tcctctttcg ttaattgtag cgaggaatag gaggaataca
     1981 aacaagatca ttaacgttag atcaagcgag aagaaatcac tatacctgga atgtattcca
     2041 tacatttatt gtcaatgttc tgtttttgag tcgctgtttt ataccttaag atttgcgatc
     2101 atagttagca ggttgattct aaaacttgga ttcaatttaa gacttatgct agatcgcttg
     2161 agtggacttc agtacatgta taaataaatc gctggataaa atattgaatg gttatctgaa
     2221 ttttagcgat ccagatccaa tatcccattg ttatgatcaa ttattcaagg gcaacataaa
     2281 caatggtaac ggaaccaata acaacggcgg cgataataat gacaacaatc tcaataacaa
     2341 ttctagtttc gcgaacgtat gaagcagata gtgggtaaca ttttttccct acactcatgc
     2401 gctgccttct gaattccagt agattatttt tcttccagta actctagctt gactttaaga
     2461 gttccattaa ttaatgtaac agctggatta gcatcgactg atcactccac cgtataactt
     2521 aatgctgcac aagcaactgt aatctggtct ggcaatggtt accatattta tttgccaatt
     2581 gaagccttca tcgaaaaaaa taaaaaaaat tttatccctt tgtcaaatat tgcaaatcca
     2641 aggtatagct ataacgtctg ctaaagcagt cagttacttt tcttgttaga gaatcctaaa
     2701 ttcttgttag aacttccaat aatttcgacc attccggcta cccggacatc tcttccatcg
     2761 actgccaata acaagttcaa tgtctgtgtt ttataacaaa acataacatc ttattaatga
     2821 agaacgctaa ttagtagaca ttagatggct cttgatggta tttacaccca ttaaaaacca
     2881 acatttatat ctgtcgttaa tttccttagc gttagaggat taaaatggta gacagtaaat
     2941 ctaaagatga tgccaccaca acaccaagac caaccaatga tgtaagcaac ctatacgacg
     3001 cgcttgatca gaacttcgtt aagacagtag acgaggtgac aaaagttcag ccaaaatatg
     3061 gacagtcaat ccataatgca attttagctc aaatacatac agtaaagaat gttatccgta
     3121 atacaatttt agcacagaag cagttgacaa gtggctggaa tcttccagta gcatactcat
     3181 aacaatttgt aaaggaatca gacgaaatca ctaagaacat agtcaacgca attgatgtga
     3241 ataacgaggt ggcggtgaaa tcactagatg cgataacaga aaacttcaaa acctttaatt
     3301 gaacaattga ttcatttaca gaattcaaca tcaatgctgc taagacatgg aattcactct
     3361 acagtgcaca acaacaacaa ttcctaaagc aataattcgt tctttttatt ttcctttttt
     3421 ctatcgcact tcatctgcca tgtttgatct cgaatatgac cgacaactat tagtttattt
     3481 taatgggaat ggtgccctga gctttgatgg ggtaatgaat cctcctggat gaggccagat
     3541 tctatagccg agtcctttaa ctcccgttta ggtacacccc tcttcccatc gctttttcga
     3601 tttatcaaat ctagacacct tatcaattta tcactaggga tttcaatctt atattctggc
     3661 agaccaatga tctgtttaag accttcagtt tcttgtttct cctcttttgc tagtgagctg
     3721 gtataccttt cagggactac atatagggtt taatcatggc aatgtccttg aacatcatac
     3781 atgccatcat ggaagcaatt ccctgaatct tactacccac cggtagtcac tagagaaact
     3841 aagttactat ccaacaacta gggttattgg tgtgttcgga gaagaggcag aaccaattcg
     3901 cgactacctt agatgtcatc acaaactttc tgaacacggc cttggaagta gactttgtca
     3961 atgtaggcat ccccggaata tcgcgctcgg agcatagaaa gacatagtac gacaaaaaat
     4021 atattgaaag tttttctctt tgtatttctt ctttaaccta ttctcttttt acgagtatat
     4081 agtgtggtat attcgcgatt gtcgcgggat atgacgtttt atattcccaa cctgatgcaa
     4141 gcaactttct tgcttctacg acctcagtaa ctgcagctac atccacaact cgtcgacgtc
     4201 taaacaaagt cgtggctgat ttgtcaatag gtaaatcttg aacgaatttg tgtttgtgtt
     4261 ctacaccgtt ttcgtcgact aacttccaac taattttccc tgttgtcgaa tcttctccta
     4321 ccttttcgaa tccaatcaac ttattaggga atcctgctgc tttacatctg ttgcattctt
     4381 gcatcttttt ctgttgcagc ggtttggatg cttccgcttg tgatattgtt gacattgtaa
     4441 taatcatcta gtaaaatatg gtaataatat atgaaacacc atgggaagag ggagagagtg
     4501 cacaaagcac tcaaattttc aatataggtt agaaactcta tgagtaccaa ttatgatatg
     4561 gtttctataa ttttcacatt ctggaaggaa atagataccg gcaatgacca tgtacaaaaa
     4621 tttaacagca atggtggttt cataacaaag tggggatctg gagcttctgg cgttggtggt
     4681 attgtcgggc tgatagaaaa ctccataccc agtattttat ctctgctacc ctgatacaat
     4741 cttctaattt cttcattttt tgctaacgaa tctacaacaa caggaaaata catctataac
     4801 gaaaaactgc atttattctg gcaaaagtaa cttgtggtta tcgccgtgcg actcgcttaa
     4861 tattatccac cactagctgc tacatactaa gatgtcagca gcaatattgg taacaaggag
     4921 aatttttccc ttcaattaca gattcacttt cttagggaca ttttgactaa taaggcacaa
     4981 atcctaaatg tctgtcgata tgtgcaaccg tgaaccgcta aacaattttt atcacaactg
     5041 caagaattgc tatcaagaca tataggacaa tttctgctat cacatggttc ttgatggatt
     5101 taaaaagatc taaatttaca caagccgtct tgtgcgagcc tacgaaattg ttacgcttat
     5161 tatgtggcac gtgggaagca ggccaaaatt acgtgaaaaa ttgagccatt tttaacttaa
     5221 catatacttt tagaaatggt tctaggaaaa gattatcaga tttacgaaca actgacaaaa
     5281 tttcagaaaa atgagaggat gcgggaaaaa aagtgctgca ttagtgcgtt gagtgagctg
     5341 agtttagcac aatattgaac gtcccaaggt aggactgcat atccttagtt agattgagca
     5401 tcaattgttg tccagtatta cttttcagca agccatcggt tgacgcgccg gtgtagatcc
     5461 catcttgttc attcgtagta gtgggaggtc catgcttatt gtatggaggt tgtatgtgca
     5521 cctgtttatt gacagagttg tcaaagtaaa actgtgctgt ccactccagg gtttgatttg
     5581 atccctcaaa cgtgcgaacc ttaatgtgta cgtggatggt tctaccctga taccagcctg
     5641 gataaatcgt agtgaactga gcagtccctt tatggtctgt cacttgatat ccacgaagca
     5701 acttatttcc tgtagtgtca aagtccttaa cgtctgagta gatgccttga ggattggcat
     5761 gccagatgtc tactcgtgct ccattcagag gaatatatga accattatct acgttataga
     5821 cattaaatgc aagacgcaat ggaattcctt gttgtactga accgtctgag ggatctgatc
     5881 tgatgtcaga gcggttaggc atgccgtcaa cgaagtatgg gccttctgtc tgttgtggcg
     5941 tacccattag tttagctaat gagggcgtca acgtgcatgt cttgtttgtt gacgcgctga
     6001 tctgagttgc agatagctct ggagatgctc ctaattgtgg aggtgggggc attgctgccg
     6061 ccgctgaggt ttgatttgta tttgtcgttg ttattgcaaa agattttggg aaattatcaa
     6121 atgcaatgtg tgttaaaagt agacttagag agagggccgt tacgatgcct attgtcgata
     6181 tcttttttgg tattttcagt tcatgagttt ctaatcccat aaacagtttt cacatcctcc
     6241 aattactatc aaatattcta tattctttat tctatttcta tatttcaccc tccaatttct
     6301 agtaggctag actaaaacgg aataaataaa tcgagattag tggctatctc atgctagaac
     6361 aaatcataat gctcgtagct acttcttatc tgcagatgca ggcttagtct tatcctctac
     6421 taatttgaat ctagatagtt gcttgtatgc aagaaatact atgaatgcaa ttattagaaa
     6481 gtcaatcata tttgctacca catcgcccca catgaatttt gaatttgcta cagtagctgc
     6541 cattttgctc agatctccag agggtaaaaa taatccgata aacggagtta taatatcagt
     6601 cacaaatgct gtaactggtt aagaagctgc ttggcctatg acaaatgcaa tagctaatcc
     6661 aattattcca aatttcttta gaaagtcgag gaattcctgc gccagtgact tctctattgt
     6721 aggacgtatt tcactcacgg taaaattatt cacttattca attattatgt tagcagacag
     6781 attttagaag aagattacta tcaattggat ttatcataca agattacatg gtatgttata
     6841 ctattttcag tcagtgaaag actatgtcca taagttaaca actaatctag tttgtagctc
     6901 aactcaca
//
LOCUS       DAOR01000239            3839 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_71629, whole genome shotgun sequence.
ACCESSION   DAOR01000239 DAOR01000000
VERSION     DAOR01000239.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_71629"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2280..2531
                     /estimated_length=252
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1149 a          624 c          685 g         1129 t
ORIGIN      
        1 gctgcggcaa tataatcaag ctgtagatta gagactgatt gtgtatattg tggttgagct
       61 tttgtaaact cgtctaggat tctaatgtag ttttgcttta ctgtatcata taagttagct
      121 gcttccgttg acctcgttgt aggggttatg tcgtctttag atttattgtc taccatttta
      181 tatcatcata atattattaa aatccaccac aatatatacg ttggtattta atgggtctga
      241 ataccgtgta aaaccatgca atgtcgaaaa ttggtggaag aatggaactt caagcgcgag
      301 aatgacagca tctactgttt ccatagaatg gaatcaagcc aaagttgtag cgacgccctt
      361 gttttcgatc ccaggcaggc aacaaacaac aatacaagtc aaaggattat ggtattgagg
      421 attatatcga tgctgataat cctctacttt cccaactagt gtatctgtca atccaaatac
      481 taacaagatc tatgtagctg ataattatga taacactgtc tcggttatag atggtaaaac
      541 aaactggcta gtgagaaccg tcacagtagg acataatcca cagggtgtag cggttaatcc
      601 aaataccgac atggaatcac ttacgggaat ttcccacaag tgattaataa cgaaacaact
      661 aaaaaagtta agttaactgc tgcggggtct caaagtagta tgattatctc ccaaaagact
      721 gaacataaac ggaagagtaa tctaagacaa actgtaaacg atgatttaac tgaagaagag
      781 ctgggagaac taagaagcta tggctacaat atatgaatac gtccttactt cgttagtgta
      841 attattggtc gaggataatc agcgtctttc ctagttatta cagatacctc gttacggtct
      901 agtctttcca accattgaac caattcatta gtcatctgtt caggtgtcag tttttttatt
      961 agtctgcttt ttccatacac atataggtaa tttattatca tgcttctcct gatattcctt
     1021 catgagttgt tccttcactt tatcagccga atttgtaacc aaataactga accgtttggc
     1081 cttctttata cacggatctg aaatgcattc gagtaatgat tgtctaagat tttgggcact
     1141 gccgatgtag agaatcttca tacacgtgtg cattttataa actccaggga attttcgaat
     1201 ggcagcggca ctagactgat taaaatctag ccaaggcctc tttatcatta ttgccactgt
     1261 acatgacttt aagataagaa cagtccattc ttagcttagc aaattataaa aaagatgtcg
     1321 tattttttta catatagctc atcgtgtggg gctttgacta tctttcagtg cctttatttt
     1381 taactgccgc ctagaaaaag atactgaatg ctttatgtca gactccaaca tgattacagc
     1441 gtattcctgg gcagccggcg aatgaagggg tttgtaataa tggagagtct aaaagattcg
     1501 caagaatcaa tagtcctaac actctgggaa acaaagggtg acatggattc attttaccaa
     1561 ccagacaaca aagttcttgc taattttgtg gaaagctcga aaatcttatt tgaagagatg
     1621 ccagaacgga atgattatgt ggtatctagt ttcaagatat agatccttgc aggaagcaac
     1681 gcgtacgtaa catggtggac caataagaca ggagtactaa tgccactatt cagagccagt
     1741 aatgatagtg ggaatacatt tgcaaaaccg atatgcgagt tggtagcctc tgtgcgttac
     1801 gaaagtatga tatataccag atcacagtat atgaaaatac caatggtatg tcacgttctg
     1861 ctcaccttaa tatacgaacc atagatataa tatttcccca ataatattat cttctctttt
     1921 cgcgatagtc ccaggtagag tagtaactgg gcataatttc caaaataact gagtctccat
     1981 ttttaacgct actcataaga gacgttgcca taggatgtgc aaggcttcca agatatctga
     2041 ccattatctt ttcggctatt ggcatattgt gatcgatgtc ttcatggatt ttgacttttc
     2101 ccttgcctct aactcctttg tatggtggat tgggatagtc aacacaataa tatacaagat
     2161 tgtttctgtt caaattttct attttctttg aactcttaga agtctcaata taaaacttgt
     2221 tgttagtgac atcaaaataa taccatgttg gatgaatgtt tggatctcct ttattgtcan
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn natgtttgga tctcctttat tgtcaactgt gccaagatgt atgttgagtt
     2581 tgctatcacc taaaaagttc tttatttcaa cttcagttaa ctgtgtacca aatcctggac
     2641 ttgcattcag aattctcata ttattaataa tgtgagaaca tcgtatattt gtgatcatga
     2701 caggagaaag ctcaaatttc gcatttccca ctaccaacca acacaaaagc accttttagt
     2761 aggttcagaa aatccattga tagaagtgga gtacaaggtc agatagagaa tgttatccta
     2821 taacacaagg agaaagtatg ttgaaaatat ttgaagaatg aagaagaagg taattttaac
     2881 accttattct ccatactagt gactaagcag caacgatgtc gcaaagcatg atgacgcaga
     2941 cacccgttag ggaacataac tacaccagaa tgttatagaa taagattaaa tacttgattc
     3001 gcatttaggt aactaatgaa ggaattgtcc aaatgagggg aatttcaaat gttagaacga
     3061 aaagtccaag atccaggtcg tagattatca gcattaaagc aagcactata tattttaaaa
     3121 tgtatctcag aaggtcgatc caggagagac atagtagaag aatttgatgg tgatgagcat
     3181 ctagtatcaa tatggataga ttttcttaat gaacatggct ggctagttga aggaagtaat
     3241 aaattgacag tcacagaaaa tggaagaatg atgataggaa gtaatcatat tattttttaa
     3301 aagattcgga ccagcaacag cgtatatatt gattaactgc gaaattggat gtgagaccgc
     3361 agtcatagat gagctcagac tattgcctca agtcatggaa gctgctgaaa tttacggctc
     3421 tccatgttct ccgtggtaaa tgcacaaatt ttgattcact cacatttcgc tcaacctata
     3481 ttaaaaccaa acttgttttc aaaatttttt gtatattatt gtttgtttaa taatattagt
     3541 gttgacggtt gacgatagat ggtgtgcggt tgtccaacag tatacggcaa gtacaattaa
     3601 gaaacaataa tatagttacc attgttgatt ctctgtttcc taacaatgaa tcatattatg
     3661 tgcattgagc aggagcaaga agttcgaaat ctcaaaaggt tgggttataa gtatatggga
     3721 ctaactggta caaatataat tcatctttat ctctagaggc gtttctaaat ctatcttgcg
     3781 tgaaatatta ttctcttatc ccttcaaggt ttgaaacaac ctgccatagc attgttcta
//
LOCUS       DAOR01000240            3805 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_45326, whole genome shotgun sequence.
ACCESSION   DAOR01000240 DAOR01000000
VERSION     DAOR01000240.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_45326"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1167 a          690 c          711 g         1237 t
ORIGIN      
        1 cctcctaact gaatacggaa gccattcatc accataaggt atatactctg caactctaaa
       61 tccctcttca actaaatccc tcttcaactc atcccgaata ccctttagaa attcaaactc
      121 gaaatttttt ataccaattt tggacgtttt ccataatcca atcgcatgct caattagctt
      181 ggaatcatga gttgcaacag caaacttaag atcctcagat tctttgttaa gtagtctttt
      241 ctcaacctta taattacggt taaatagtat gtccaataat ttaatgtaat ttgcattaac
      301 ttcgtcatga gatgcaaatg caatttgctc attttcgtga tatgctccct tgactaatct
      361 tactttgcca cccacttcaa tcagatgtaa aagatcactt gcgctacgtc taagtgcaga
      421 ttggagaacc acacctatat catggtgacg accaatcagt tcaaggtaca tcattaatgt
      481 gtcatcagta tatttggaag actccatatc gatccaaatg aatctgccaa actctattgc
      541 tcttttggta agagctctca agtttttcaa acataattca tagctaacgg ataacccaag
      601 ttgtgacggc ttaacagata tgcatccttc aatttgactc aaatacagtc tttctaatag
      661 attagaatat tcttggatag ttcgatttat cctttcttct tcggtaagat cttcccctag
      721 ataattcaaa attgcttttt gtcctttcat attgcagttt tttgctgcca taagggcatc
      781 ttccatgtcg ccccctgcag cccacttttt gaccaatctc aagagtagcc gttccattag
      841 taaataatta tggctatccg aatgattagg ttgattgtcc atattcatag agacatatct
      901 atagacatta taaacaggaa tgtgtcatat agttcctgaa gttcattgtg aaaactaact
      961 ctagtcttca tagatgtaat tggagactta aatggttgac tgataattat ttaagtggtt
     1021 aacagccata gtcctaaatt ctttgttggt aaggtctaag aagacaaatc atattgagga
     1081 cccaagagat agacgaagga agtgtttgtg atattccgag gcatttcctc gcttactatc
     1141 aaagatgttg tagatgcttt aggaattgca gggaatttta ttatggaaga attgaccctt
     1201 gtgacgtagc attctccagt gatggcggtc gtgtctgcaa taccacattc atattcatta
     1261 tttggtcatg cctgttcaca gtgagcacta cgttatctcc aacagattta tgcacatcga
     1321 tgtaactgat gagatcatct atactcctca aactatgtcc atcaattcct gttataatgt
     1381 caccgacatg ttgcgtatta gaaaattcgt cttgtgttac tccttgtaga cctgctttgt
     1441 ctgcgggact accagcttga attgaaccga taaccactcc cttgtagttc cttggtaaac
     1501 ctacggtctg tgccaagccc ggggttacgc tacctccaga tattccaagc caagcatgac
     1561 tgtagcatcc atgttcaatt aatgctggta cttcacgtat gatggtgttt gatggaattg
     1621 caaaccctat accggaaaac tcgcctgtag aagaactaat tgcagtattc attcctaata
     1681 cttgtccatg catattcagc aagggacccc cagaattacc cggattaatc gcagcatcag
     1741 tctgaattcc atttggtata gagaatccgg tgtttgggtc tggcaacaaa cgccccattt
     1801 gactgacaat accggttgtc atcgtatcac taagtccaaa tggattacca attgctatca
     1861 cttgttgtcc aacttgcaaa ctagatgaat tggcaattac aagtggtaaa agattttcag
     1921 tcgagaaatt gtcggttatt tgaattatcg caatatcact aaatgcatca gtaccaacca
     1981 cctttgctgc ataggtattt ccatcaacaa atgttacatc aactgtttta gcgtttccaa
     2041 ctacatgatt attggtaatg acataacctt gcttatcgta tataaatcca gatcccaatc
     2101 tagtagattg tccttctata gggttaccat ttatgataat ctgtatgtta ggatttgata
     2161 gtgtgctcgt gatctgaact acagaatttt ccacttgttt gaatatacct gttagagata
     2221 agatttgaga ggagttatgg gagcttgatg acttagtaaa ggaagtagca gtaacagctg
     2281 cacctgcaca atcaccatta ttattatttt gtcctgttat ttgttcttgc agctgctttg
     2341 ctccatttat tattgtagag agaacgggca gtagtaactg ttgtggttgc tgtcctatga
     2401 gagatagtat tcggctattg gtgatagact gaatcgcaat aacagctaca agaataacaa
     2461 ttaccgcaat caaagataat tctctggctt tggtcattgt gaatcgttta gaattagtac
     2521 gaatatagtg tcgtcgacat ataagtataa gtttgttatc gatatttatg tctggttttt
     2581 aggatgcttg atgtagtgtt tgactcttta aactgtggag ttatgtcctc agcattcatg
     2641 ctggtgtcaa atcttgaaca attcttggtc cttgtggagt agaactaagt atttcattgg
     2701 aaatgatggt atgtcttctg attaaatcat ttgaaataga aagcaggatc gtcgtacctt
     2761 gatttcaaag tgattgtaac ttatccagtg tagacaaagc gctttttgta gtgtgttcgt
     2821 cgaatgaatc gcatatcttt tatagcaata atcataacat taatccaaca tatcagcata
     2881 aaatgtgaca accgataaag aaaaaaacaa taagaaaaat attttgatag tagatgatga
     2941 aacagatgtt tgtttgacac taaaggtggt tttagaaggt aatggattta aagtttatac
     3001 gtttgttgat cctactttgg agctggagaa tttcaaaagc agcaggtatg atctattaat
     3061 tctggatatc aaaatgccag taatgaatgg ctttcaacta tatgcacaaa tgaagaaaat
     3121 agatcttaat atcaaggccc tttttctgtc agctctaacc gacttacaag agtacgatgc
     3181 atttaaaaaa gaagtctttc ctacagaagg ccgaagacaa tttattcaga agccaattga
     3241 aaatgaagag atgttagtaa gaataaacgt agcaaccctc aacattgaat atttagctag
     3301 atatgcaaga ggtatcccta gctcgttggc atatgagacg gaattggtta aagtatttct
     3361 tattttgtta ttagaaaata cacaatacgt tcttactacg atagattgca ttctgataag
     3421 tcggcgaaat cagctcgaac aaagatattt ttataaagat tcattcgtga gggggtttaa
     3481 gtattcatta agaaaacaga ctattgttcc ataaaatatt aatggaatgt agaaagcggg
     3541 aatgtgctct ctgtgaatga cacgaagcga attaatgcaa tattaagtga cttaaattga
     3601 taagaggtag ctgagataat aggtagagca ctatgggtga gctagtgtta ggctgttctg
     3661 gatggaatta tcctgataat caagataaag gaggctggac tggtgtattc tatccagaca
     3721 aggatactaa gagactgcga tattactctc aattctttga tacagcagag atggattcta
     3781 ttttttacga aaaattctat tcgca
//
LOCUS       DAOR01000241            2785 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_135436, whole genome shotgun sequence.
ACCESSION   DAOR01000241 DAOR01000000
VERSION     DAOR01000241.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_135436"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    290..464
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          776 a          511 c          499 g          824 t
ORIGIN      
        1 ttcataattc tcctatcccc aatctctctt cattcaagcc gcctacacta ttttagattt
       61 aaacgataat agcatgtaga aaataagttg agtggtatca gaataaatct caataaattt
      121 tatttctttt gcgtataaag gctgaagtta cactcttcgc agaaatagta caggctgtca
      181 aaatcggaac ataagatcat ggtttcagaa caacgtgggc attcaatttc agatactaat
      241 tctgtttgtt ttgtcttctt tctattttgt cgatgataat acagtgactn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngataat acagtgattt
      481 attcgaagtg ttggtcatcc ctcttcctgc gatacctttt attcgagctt tatttctcgc
      541 tgatgtaatt gatcggagct atttcgtcaa ttttattctg tagatgcaat ccacgcttgc
      601 ctttctcagt tgtcctgtaa gtgcacctcg ttcttgagag gctgctactt aatcagtctg
      661 cgccctatga ataaaaatta gggaatttct tagagtgggt aataggtaaa tctaagatta
      721 tcgataataa tcaagtttgt tcttccatct attgtttaaa tgcaataata gtacgccttt
      781 ctctgtgatt ctgtaacacc gcccggcctg cttcgtaatc gattctggga cacaggaccg
      841 ctggtatcgt gtatacggat atattatgta cagatataca tattatgttc atggtgtgtg
      901 tcattcatga ctactagtaa cgccattact gctgcaatag cgttaatatt acttgtattt
      961 atagttgggc tatcatttgt cttggtatcc atgcgtccag ttcattagct attagtttct
     1021 ctttgcccat tagcagttat tgctacgtta tcactatcta atttcccctt cttttcatcc
     1081 cattagtact tcatttgcag gtctagatta aattctagat aagggaattc tatccaacaa
     1141 aatctggtgg aacacaaacg agagcacggt agaacgcctt gtaggattaa gcataagttt
     1201 caaattatat ggatatcctt ttgcttcggc agccgactaa tttcaagtga acatccagac
     1261 ccttcacata aactcgcaaa tagttccaat attcctctat tctgttcagt gtttgcaaaa
     1321 cagatctact ccatctggat ttacaacaaa ttctatggct gagtgttaag tgcacaacaa
     1381 caaacagccg tgatagcaca agccagtact gataaacgcc ctgcttcttc tgagtgtttt
     1441 ccaatctttc catggttcat gggctagtct ctctcagttg ctaaatgcat ggtagatagt
     1501 atcatacgta ctttgctgaa tccatctatt gtttttcgct aataactggt tccaaccgaa
     1561 tatcattcca gctagcgatt tacacttaga taaatgtacc acaagtaaga ccatatcagt
     1621 ctaagataga tgatagttga gtaaaaagga gtcttccatt aatgcgtgag gaaaagctga
     1681 gataggaact acacaaaaga acttttcttg aataagattg gagacgagaa ttcatctaaa
     1741 ttatgagcta tagtagaagg atttattgga tatgagaaaa acgggttcag gttggtcaga
     1801 tattcactta ggagagtgta cctaaaatct aatatacaaa gcttgtcgaa ctcattatat
     1861 taacgtgata aggacgaaag gactgaacct caggaaacaa aagcggacga aaacagtaac
     1921 atctaataaa aaaaagatat ccacaaacga acaccctcca ataaattctg tatcgtcttt
     1981 gcggggagct ttagttcatg aataaatcac gtattctcat aatagctggc ggcgttgtta
     2041 taattgtcgc attttcacta tataccttta gctctcactc aacggcacct agagctggta
     2101 caaaagagaa ccccgttaat tcggtagcat caggtactgg tggacctgcc tccgcaacag
     2161 tctctacctt tgtgaatcgc gaaaatccag gaatctttta ttcaatccag tttcctagca
     2221 aggcaagcgt ggaacacgga aatgcgtccg gaagttatat agcaaaattg ccacaaggag
     2281 ttttctcagt ccagcttgtt gatataccag acacttccaa cgttcaactt attctgttaa
     2341 ctcagataca gcctgctctc aaatcatcat tgcaagattt taatcgagtg ggttataatc
     2401 aatttactgt aggaggccat agagcatggg gtcttacata cacatggaaa aatacaacaa
     2461 ttggaatgga atctataaaa gctgttgtcg aagggtctga tcactctgcg gcaataacat
     2521 tttcagggcc gagacagggg tttggagatc aaaacacaaa ttctaccgtc ataagacctg
     2581 ttgtggggag cttccattgg atcggaaaat agatcggcat cgtgagaaag tggttggtgt
     2641 tctttcataa aaatgcaatt tgagattaaa tacttcatag caatcctgtc ggctatcttg
     2701 atttttggag ggttgtattt gaagttcctg tatgacgtgc ctgatgcaga gaccatcacc
     2761 aatatgtggg tcagctggtt tctca
//
LOCUS       DAOR01000242            2552 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_49096, whole genome shotgun sequence.
ACCESSION   DAOR01000242 DAOR01000000
VERSION     DAOR01000242.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_49096"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          789 a          502 c          451 g          810 t
ORIGIN      
        1 atcttgtcgt ctttccttca ttttgtaagc gcacaaactc ttttgaagaa tttagtgcta
       61 acagaacgct acgcctgtct ttaataacaa attcgctatt ggaaaatact gaaatatcgc
      121 cttcaggagt cataactgtg ggactcgatt cttttaatga ctccaaataa tgtctaattg
      181 acagactttc atttcttaaa taccggtcaa aagctatatt tcccgtatta cttagttgag
      241 gaaattctcc tgagctctta aagttatgca aaacatactt tttaatatga ggcaaaaaac
      301 gacgagacac gacaattccg gcccctccaa gtctagttat atttcctata tgatatggtc
      361 ttgaatatag aatgtcatat ttgttcaaag attgggaaga tgatgtatca tatacaaaca
      421 actcatttgc ttgattgaaa atatctttta ccttataacc tactcctctt ctgatatcta
      481 aaatttctaa aaacttcata acagagtcag accatgcttt aagcgtctct ttctccagct
      541 cagcaagatt gtttccatat tctttaacat ataactttat tattgtatca tttttcttag
      601 gtacaatgta atcgtgtatg taccaatcat aaaacaatct tctatcacca aggtcgcttt
      661 ggttattcac atcttttcca taacctatca tggcaccttc tttgtcaaag aattcgttgc
      721 agtattcgtt aatttgtccc ctaagtttgt cctctagctc atcccttgtt gctctttctt
      781 gatcgaggct cagacaacaa tgcttgtatt tcttcttact accacatgga cacggagaat
      841 ttctgtcata tttcaattta cagagaatat aaacatacaa tgtgaaaagt atttctgaag
      901 ggtcaaagac tgaacaatcc gttttcgcca acagcatatt tttggaaaag gataagcgat
      961 taatacttac ttacttagcc tgcatgttta ctgttggatt cgaatcgtag aggtggatgt
     1021 gcaacaccgt acatctcgtg ctattctttt ctctcgatgg tatccaaatg gagtatactt
     1081 ctctaaattt gagccatatt cgacatgcat atcttgaact catttccgag ctgtctactc
     1141 agacatgacg gtaaacatgg gggctggata aatgaacaaa ataataatag cgtggccaat
     1201 tatggcccta cttgctgtaa tggttccaaa tttggcccaa gctcatacac aagattacaa
     1261 tgatggatat agtcaaggac aagcaaacgc agcaaatggc ataagcattg atacaggata
     1321 tctcaattct cattctaaag actggcgaac aggatataac gacggctggg ctgcagcacg
     1381 cggtacagtc ggtatcccag catcaggcaa tacacaacaa agtgaatcaa gcgctataaa
     1441 cacagcaggc aataacaatc atgacacaca agcctataac ttgggctatt cagacggcat
     1501 gtcttgagac atcttcctga aacaccaatg aaattcttcc tgcaagcaag gcagcagaac
     1561 ttttgatgga tatacgcact ccatgcttga tttcggaagg gaataaagga gaagaagaaa
     1621 ccgataatta catagtgact ccctgggatg ttgtcatgaa aacattgaag tcagaaagta
     1681 cgccgatcat taccaattaa gtgagtctag ctcatttcaa aaccgctgct gtaattatct
     1741 acataatctt aatcttagcg tataggtcaa tgcagatcgc gcatgaaaag atataagtgc
     1801 taaaatgaga atgttttaaa tggttttatc gattcgagat atgtcctcat taaccttgga
     1861 gtgatatcgt ttgccatctt aagcattttg tggtgaaact gcattctgga taatactagg
     1921 tcatactcaa gagagaaata ttaaggaatc tgcgatgatg atcgagccga ttaacacaag
     1981 tgtaagattc ctcagatgga ttttgaagcg tatcttgtct ttttctatag tcagataatc
     2041 gctcacatga tttttatctt ataaggattt ctgctatttg ctactttgtg ttactgtata
     2101 aacttcagtt ctgctaatgt gacaccgaca aacacattca atggtgtcaa cctactctat
     2161 actggcgggt cggaatgctg tttgatgtga cgtatacggt atctttggtc gacgaagatc
     2221 ggaaacaaag cgtgtagaat atttgttcct gttatctttc cgtggcttcc tatataatac
     2281 tcaggttaga tgcactcaca agttccatta ttcatttggc agtttgatcc gaatatgtag
     2341 aagacgtgat ttcgtatatt caacaaacta tttttgaatt tgaactgtgt tgaagacagg
     2401 tcaatgtcaa acagacttag acaatcaagt cttcaacttc aatttatcga acctaatact
     2461 atctttaacc aaaagagaag agttatcatt acggcaccaa cttctattca tgagacctaa
     2521 tgtgttagac acagcatctt attgctacct tt
//
LOCUS       DAOR01000243            3127 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_72438, whole genome shotgun sequence.
ACCESSION   DAOR01000243 DAOR01000000
VERSION     DAOR01000243.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_72438"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          910 a          630 c          592 g          995 t
ORIGIN      
        1 tagttgaaat ccgttcatgt gtggtatatt aatgtcaact aataaaagat caaagaaatt
       61 tggttggaat tctgacaacg ctgccgtagg atcgttatat gtatgcactt caattcttct
      121 gttgttcgta tcgatattac catcttctat gcccatcttg aaggtcgtag tgatatcggc
      181 atcatcatct atgatcaaaa ttcttttcca aaatcgttcg ttctcgcttc tgccagttgc
      241 atcaattcga gatgtgctat taatgcgatt tttagacgtt tttatcacat tgtaaattca
      301 cacttttatc tctttccaac aggacatagg ccgtcatgtt atatcaatta gacattgcac
      361 ttaaaattgg ttatgtaact ttattacatg atagcacgaa gcaacattat tttcaattta
      421 atattcatac cttttttttg gtctaacgcc actgaatgaa agggactatt tctactactg
      481 tgtgcagtta gtagcctagt gctcctatag catacgcaca acaccatgac ccaagtaaca
      541 tagtgtccca aatcgagata tttggtcaaa aaaagtatgg aaataaccat gttagagtaa
      601 cttatgatga ttcaggtggc tggtacgacc acgtaatgcc tcctataatc agtcaatcta
      661 gtgatccgaa atctgatagc cttctcggta atgttggctt atgtggtcat gctcctaacg
      721 gtggctatca agatcgatgt ggttatggtc ctcgtttgcc tcttttgatc atctcgccat
      781 acgcgaaaat aaatttcata gatcatagca taacagacca gtcttctatc ctgcgtttta
      841 ttgaagacaa ttgggctcta ggaagaattg gaaatcaatc acttgatagc aaagctggct
      901 cactcatgaa tatcttcgat tttcccccag cagctcatca tgcaaacaag ctgttcctgg
      961 gtccttcaac tggattgcag aattccagtg tattgaagtg attaacccgc ctctgtctaa
     1021 tggaattttc atgcttaagg actcttccag agtgctgctg ttgattgcat gagcatggtt
     1081 atctctcgga ttatcgattg actatctggc tctcggttac atcataattt ggtagttggt
     1141 taggggtagc gacgggtgga ctccaggaat tggccacgca aatctggaca tagccggata
     1201 aatttgtgca atcaagtctc ccctttctac cgcaacagat tctacaagac gtgacaaagg
     1261 cagatgctta atcagttttt ggtcttaagg aggataaata aagagtcaag tacagtgaca
     1321 aaagtaagcg tcaggttgtt cttcccctat agttctcttg attatttagc gtactcttta
     1381 cggtaaacgg tctgtattgc atgctggcac gaattcttga attgtaggaa tggtattcgt
     1441 agttgttagt gtccgctacc gccgaagcca tcaaagccgc caccgccgcc attagtgttg
     1501 cttctgccac cgaagccaaa gccgccgcca ccactattgc tgccaccgcc agtaggcgca
     1561 ttggtcatct tttggcatga aaaataatct ttcccttgct ctgatatgca acctgacaag
     1621 aatccttcgt agtaagccct accattagta ccacacccat tgtacgtggt ttgattgtaa
     1681 ggatgaactc gtccatcagt aaaacctgcg ttataacaag cattattcct ttggtcaccg
     1741 ctgctgccgc cactattact cccatatgca accaagttac ctatcgatcc aaaaacgata
     1801 attgtactaa gaactagtat cgctatgaag tatatttgtc gcattctatt ttagagacac
     1861 tcccgttctg ccaattgttt cttgacctga tttcgatcta tgttgagaca gagctaatgc
     1921 aattatcata gaacgaaaac tattaggaca ttcactactt aaacttttaa ataagacatt
     1981 tactaattat tctatagtaa aagaatatgt ataattatct atataacacg tttacaaaaa
     2041 tcaaatgaca gaaatgctgc agatgcaaca aatccggagc cccaactgct acagccaaac
     2101 taatgttgtt atctgaatca aaagacttta tgatactgga ctttatagca atgtatagca
     2161 cctaacttgt tctgacagaa ttggtgtatt tgcggcatga cataattcga gatgataaca
     2221 aatcctccca ctactgctac caatgctatt gctacaagta caataactat aggctgtcgg
     2281 acggcgctga tcctctgagt gtgtggtaca ccgtttagca tatgtaatct atacctattc
     2341 aaagatataa aatcatctga aaaaagacta aacaaatagc aaataaattt aacaatgtca
     2401 aattgtaacc tttatttcta aaacgtttag ccaactgatt caaatgtaaa ggggaatatg
     2461 atgccataca tccacctagt ttttggtctt agttccttct taggtaaacg taatctgtat
     2521 attctgaaat cgttaagatg ccattcgtta tcctggttgc ctctggattt tctttaatat
     2581 catcttttac tagaagaact tgaagcagaa gtcgtacttg tattcaatga acttgtattt
     2641 gctcgtatgg tggaggcagt agtagtagca taggaatgtg aaagaccaga atgagctgca
     2701 agcttttgtt gtatgggtcc aatgcctgtt gtccattgcg atgaagaaaa aaattcatgt
     2761 ccaagatctg gatatgtaat aattagatga tcaggatgat ttagttctgt taatctttgt
     2821 tgcaaaagta acccttgttc aactggagtt tgactatcat tttctccttg cagtataagg
     2881 atactaatac ctgaagaaac gtttcctatc atgcttaagg tatcatccaa ggcaagatgt
     2941 ttgattgttg ctaccatctg tttttgtaat tcagtatgtg gttttttctt agtttcattt
     3001 tgagaagcca tcttgctaca gtatgggctt taaggatggc aaaagcaatc caggaaccag
     3061 atgtcctagc ggtcacaggg taaacttttg tgctggatgg aatactgcaa caggaaatcc
     3121 acctacc
//
LOCUS       DAOR01000244            5545 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_85470, whole genome shotgun sequence.
ACCESSION   DAOR01000244 DAOR01000000
VERSION     DAOR01000244.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_85470"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1797 a         1054 c          935 g         1759 t
ORIGIN      
        1 atactgtaga agatgtgagg tttattattg ccacactggt gtgttctgtc cttgttgcgg
       61 tatggcatta cgaatgtcac cgactaataa aagagataag gaaaggctta ggcaattaca
      121 acttagaaga gaggaagacc aagatagaat aatcaggatc ataaagagga taaagaagta
      181 gcctgtcaat caaggcaatg tcatatgtaa taaatatcct tgggcactta ttatccatga
      241 ttataacgaa gagaaataca gatctctatt gaaaaaagtc tatgatagtc tacctaatga
      301 aaatggagct ataataatta gtgattggtt gttaaatgat gaaaaaacag ggcctatacc
      361 cgcagcctta atgagcttga atatgataat gctaaccagt ggcggaagaa attattcttt
      421 tgtagagata tctagaatgc tttctgatgt tggatttaag aatatagaaa aaagaccttt
      481 agctggccca gcacaaattg tcataggata caagaacaaa aagaaatagc aagatctcct
      541 aatcctgttg attttttcca aactattagc tatcaatttt actagccaaa gtatcgctgg
      601 caaaaatatt gtaatcggag ctggttcgat aaatatccgc gagcatcagc ttgcaaagat
      661 acctaacgaa tatgcagaaa gtctgagaac cttttccgag agtatttcta aacagctcaa
      721 agatcatcac aaaccaatgg aagaagtaaa ctcaataaac caaagtatga acgagtttgc
      781 taaagaagtt gaagatatca cagtaggaaa agaacaagag attgactatg ccaagcagac
      841 aaacattgaa gcaaccaccg ctggtgttat acaaagcgtc ttaaatgctc taccacaagc
      901 ggcagaaact gcagcaacat ttactccttt agccccagtt agcaaactaa taggaaaagg
      961 cgttgaagtc gtaaatgcaa tacagatgaa aaacagtagt ggctgacatg ccaatactat
     1021 tgattaaaga tattcagaca caattgaagg tcaagttata tgggtagtca cctatcaaca
     1081 ttgacgatat caaaggaaag gtaagtggta ttagctatga tagtactgga agactcagtg
     1141 cagaggttat caatattcaa ggaaatctta ctattgttat taataatcca acaaaagaag
     1201 ttatcgcaga actgaaaaag attctgcaag ctccgcttgt aggttctgga atcaacgatc
     1261 aaggtatcac aacgcagaat gtaaaggaaa caaagcagag cattaacagg atcaagagtt
     1321 tgctatcaaa agctgacaag agaataggaa aaccaactca ggcaatcaaa gcagataatg
     1381 tggaaatttc tcgtaaagag ctgttattga aggacgctgg aactatggct tatgaatatc
     1441 tgcagaatga ggactacggc aatgcgttag agtggtctaa taaggccata cggataatcc
     1501 taatcataat atttcacggt ataacaaagg tttagctcta tataatttag gaaaatacaa
     1561 agaagccata aagtgtaatg ataaggcttt acggatagat ccaacttatg ttcatgcatg
     1621 gactagctgg gaaaacacaa tgatatttct cttgagcact cagaaatcac tgaggttgtt
     1681 gcatatagaa atgcagacat ggtatcaact gtcgattttc aaaatacgga atgtgtcaag
     1741 tatcacataa atggccatgg atattctctg gttggttgtt gttgtaatta cctgcaatta
     1801 gggtcaataa gttatttact cgggaactca ggcgaattat ttttcaacaa ccaaaaaaag
     1861 aatcgattct ggaaaagaaa actagtttag tttctttttt ctcattttct tttcttaaaa
     1921 tacaatacta atgattgatt ccggattaga ggtatattct tccatcagta tctgtaaggc
     1981 gcgtaaaata ggtgcttcaa tatttggttt ttctagacaa tcttcctcat aaagcctttt
     2041 tgctatggat aaagttgaat catattgcgt ttgtaataaa tcaagcaatg tgggaattat
     2101 aaacgacctt gaaataatct tatgcctatg aataaataaa ctatgatggt agctggttcc
     2161 ataaattttg gataaagcat atctactcat tgatgtgatg ataaagccac aagttatttt
     2221 gagaagatac tgaaaatcct acgtaaagta aagtatattc atctcaggaa ttatttgtta
     2281 tcaaataact ctactgagtc gtttagcact tttcaatctc aaggatacta ctacgcttca
     2341 tcatgctacc ttcctattcc agtagctatc cttaaaacgg cttcccatac tccactttga
     2401 catctaagaa atctttcatg ctcccgccat acttagtcaa tacctcgtct ttacctttaa
     2461 tccatttttc aataatccaa attctgttat ctattgacac tttaacataa tgttccttat
     2521 ctctaatttc aatattccat agcttgggat ccaacgcttt gcttttcact tcattacaca
     2581 caaacttata ctcaattgta acatcgtcgc tagctgccca tgtaatataa aaataatctt
     2641 cgtcctcata atttatgcgt gcaaatttaa taggacgata acacgaagat aacaaagaga
     2701 aaaaggcgta acaaaagaga aacactggaa aacctctcca tccatccaag aactgctggt
     2761 ttcaatccga gaacaatgaa ctatttcgat ctgctaaggt agaacaagct attcgtaacg
     2821 ctagcattaa tttctcatca gagctgtata atatgataag aaatggcaaa accaaaatca
     2881 caaatcaaat gtgtgttatg cagtaaagca atcgcagaaa taatattcca atagaatgat
     2941 gatggctaat tattcaaggg caggctaaat gtaaatgttg cacctttacc agcagtatta
     3001 ttttcagccc atattctacc tccatgggct tccacaatac ctttagaaat atacaaccct
     3061 aatccagtac ctttgtctga cttcttagta aacttcgtaa acaacctagg caatatttca
     3121 ggatctatac cagtaccggt atcctctata gtaataatag cagaaccagc atctttcttt
     3181 ttcttagtaa tagtgatggt tccctccttc gtgaaatgaa ttgcatttct caacagatta
     3241 gaaataactt gggttatcct atctttatct gcttttacaa atatagtatg ccctttctca
     3301 gaatcataca gtatctttac ttgatgatta ttactatttt ggcttttaat atcttgcaat
     3361 gcattgagta ttacactatc tatatcaact acttccttat tcagatttat cgataggctt
     3421 tcgattcgag cgagatccaa aatgttccct gtaagttcct ttaaccttct tgcgtttcta
     3481 attataaccg ataggtattc gtcgtacata ggaggagtta tattctcttt tctagaacga
     3541 agaatctcag ctaacccaat tatgggttga ataggtgttc ttaattcatg agctgctata
     3601 ttaataaact ctgtttgcgt cttatcgcgg acctttaatt gctcacataa ttcacttact
     3661 tttccatata gctctgtttg tttccaaaca ctttcaaata tagaaacgta agacaatacg
     3721 gttgccttcc tatttgaata ggctcccaaa cccattgctt cataagaaga ttccttggta
     3781 tcatctttta attcaacaac aagggaggat tttctatcca ccactaaaat ggtgacctgt
     3841 gtttgtagac ctggctcgct gtaacgtatt tcaatattat gattctgtcg ttttaacatt
     3901 tgtactgtct tctcaattag tggttccttt ggtgtcagga ttctggtttt cacaccacgt
     3961 tctactactt cttttaatga ctgaatcgtt cctaatttca tttgacgatc gaatgcgtta
     4021 gccgtcgaat atattatcaa gatttcatct ctagctgcca tcccgatttc aaaagcaatt
     4081 tgttgtattt caacagggtc tcggatcagc tgtaatatat ctggcttatc tagtcctgtt
     4141 tctttgtcaa tttctcttat ttcttgctca gttggaattg ctctattcca gaaagggtca
     4201 gaaataaatt gttgttgctg ccctagtaat tctctaaaat catttccata tacacttctg
     4261 aatcttttaa acataggaat acaatccatt gtatcaaact tgtaaggagt tccgtcaatt
     4321 acttcctgga ttatggagga tttttctccc tcttttggtt ctgtgaaagt ccttccacat
     4381 aatgcacatt tacctcgtga ttcattgtta ttatttatca tagcatattc ttacttcatg
     4441 gatagggtat acgggactac ttaccttccc ttcctccaat cggtaatatc cggcctctct
     4501 attaatggtt acttaaatgc gcttactatt acacacacag atttgctcag gctggcgtac
     4561 aaatacatca aaatacccac taccgaaatc aactcacctg cttgttctat ttcggctatt
     4621 tacacagagc aatcttgatt tgattgaccc taaaactttg tcttatccca gtaacaaatc
     4681 aaaaatggag gtcatcccac attccggtca aatattgggt atagcactaa ctagatgctt
     4741 caccgtatac tgaactcaaa ccatgcatgt gagtacattc gtatactgct ccattgtacc
     4801 ctcctacttc tctaactatc cccttttgtg ctaattcaag tactttgata gtacagtttg
     4861 taagtttcgt agtagaagaa ggaagacctg tacttattcc agttgcaaca ccaaatgcaa
     4921 caataattgt taatccgatt actccaatca caattataga tttagttttc gtgctgctct
     4981 ttgaaactgt tggtcttcgg agttcaattg attctaatgt catagactat tcacctcgga
     5041 cgcccaactg ttattttcaa acaattaacc atacattatc gatccgcttg cttagggata
     5101 tactgttata ggatgctgac gatatatatc ataagcattt gatataaatg ataattcaaa
     5161 tacggataat gacttaagtt agcaattact ggccattttc aaaactattg tttactttag
     5221 tgaaaattcc aacggtcagg taaggctaaa aaattagcct taaaattagc ttgtcaaatg
     5281 ttgactcttg tggaattcag atgggtatat cgagtgtagg aactacgcaa tcatctatct
     5341 tactcaatgt tgtcggacgg cagtcctaaa cagctttgta tgtattcccc atgacacacc
     5401 tacaactatt acagtaatgg ctatttcaac aattatctat ctcctccgga tctgtgaatg
     5461 aggatgatca ctacataaaa gtacttgtag tacttacata gataggtgtc actttcaaat
     5521 acccacatag gtgacacttt tctgc
//
LOCUS       DAOR01000245            5765 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20585, whole genome shotgun sequence.
ACCESSION   DAOR01000245 DAOR01000000
VERSION     DAOR01000245.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20585"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1708 a         1095 c         1171 g         1791 t
ORIGIN      
        1 tatgtgtgta agtacaacag ttagttaact actcccaaca gctattttgt tgcagtttat
       61 tgcacagtta cgcaaattca gctgcctata gaacctcaga attcacttct gacattgtat
      121 agctctgcaa gtcgattcac atcccaacgg tttacagtct agttatctcc cgttgtgttg
      181 acagaaccca aaaacgtcat gtgacgatgg cctacatagg cagctgtagt ttagttaatt
      241 aacttcggcc agggagttct tgtgtaaaaa ccgttatatt tgatctgctt ttttggattt
      301 cctgtttttc cttctggatt tcttgcggac tcttcggact tgatggttta tgaatttctt
      361 gttgggtagt agaagaagta gcatttgtca tttgtgtact attgcaatgt atagcagaca
      421 cattggtttt tccaacaaat tgtactagat tattattatt acttttatca tataccactt
      481 tctttagtgg gatagccgct agtttcagcg gaaaaaatgt tgctataaat atagtactac
      541 aaaggaccat gaataatccg aacaacaaaa ttaaatacat catatgttgt ttatatgtga
      601 gaatatcttt tcgttatcat atatctatgt tctgcgaacc ttagtttaaa cgtcttctat
      661 attttattgc aatgaagaag gctgtactga aacaaccaac agaaaagaga ggaggaccat
      721 aggcggcata atctaaagat gagcccttcg tcctgctact tcctgaacat tgtcgctata
      781 aaggtggacc agagagagga tgaatgatat tcgccagcac cccacactat agatggatta
      841 gagggatcta ctgcagcccc aaagtagtct ccgaatctgc tggtcgtttc aggatatgtc
      901 ccaattttca atgtcaaggg agaactgtcg agtggacctc ctgtcgcagc ctgtccggta
      961 gcaaaaatac tgggataaat gctcaacgaa gaagcgccaa atactacaag tagattgctg
     1021 ctaccatcca tacgtaaggc aggataaaag tagtatgcac ctttcgctcc aacgtcaaag
     1081 tcctggctaa gagagccgat tgaagtgttt acttgaatta gtataataca agaacgagtc
     1141 tgcacatctc cagtaggagt acaagcatca ttaaaagtga gcaagagtct tccctgatac
     1201 cacgcagtat ccaggatcct agcgtcgttt gtgtcaattt gaatactagt acctcgttgg
     1261 agtccgtttg gaggtatatg cgtagtatgt atcggataat ttacaatact ggtaggcacg
     1321 ttcgagagac taggaggttt agaaaaagaa tataccgtgg cgtgagtgct cagatcgtcg
     1381 ccgactgtcg ccatatagag agttggagta gaactcagtg actgaattgg ctgtaatgaa
     1441 aatattgagg tatttggtga agatctacta taaatcggtg tgctagtggc accgatcata
     1501 taatttttgt ttatgatata atattgcacg ccggcgtagc taccattaca ggcggaggca
     1561 aaaaggttga cacttactgc aaacttatca tcgttaagtc ctattgtagg ataatcagga
     1621 cagttagaaa atttaaagtt aaagatttta aatgcatttg gaatactcga agtggagacg
     1681 gccaccttga ccgtatttag agttaaatca gcgatcgcgg caaaccatct accacttcca
     1741 gtatcataca ttatacgtgg atcggtaatc tcatcactgc tgctaataat aaaaaaagga
     1801 tgaagcgaaa aagtggatat agaacctcct gttttggtaa aggtcttacc ttccagattg
     1861 ttcatttcca ttacataatt aggtccaata gctacctgaa catccggcgg ataaaagaat
     1921 ccgccgattt gtccctcaga aagcccgttg aaaccaatcg aagtcgtagt tgatgtggta
     1981 gtggtggtag tcgtagccgt agccgtagtc gtaggagcag ctgcaatcgg agtgttgtct
     2041 aatagattgc tattattggc ctcttttgag gttgatgttg acgtttgatt tccgaaaggt
     2101 aactcgaaag gtactgataa tgacggcagg aatgaaagca ctcttaactc ttgcgttata
     2161 gtgggagtag taccgatcgt ttttagtaat tctgttcgta attttatatc ctgaagggtt
     2221 tgaaaaccaa acggagttcc cggcctatga atttggattt ctgatcttat gttgggcgaa
     2281 gtgagattag ccagatttct tatacgtacc tctgcctgaa gccgaacatc atgcgaatta
     2341 agtagtccag agctactacc atatgccaag tttgattgta atatgttgtt tttaattatg
     2401 gtattagtaa tgaaaggtgt agaaaataat aataaaaccg ctgtaataat gaatattgac
     2461 cctttagtac tttttttatt gatagtggga gttattgcac taattgaatt agcgttagtc
     2521 tttccggtcg taagcgcctt acaacaagat tagttattaa aaatatttat tgattgtcag
     2581 attgctaatt ggtataatta ttagtacaaa caaagttttt acgaaatttc ttgttacaat
     2641 cagtgtatag agtgtagctg aactatctgt gatttcgtgt gtttcatttt tacttttagc
     2701 ttctcgcatc aataatcaat caccatcata gacagtgcat ttcaacaaga ggtagtgcct
     2761 ttgctagggt gagcgtcatg caccgttagc tggaacttgg gtaggtacac atagttattc
     2821 atggttgaac tatattgtgt tggattttct tactttgcta ggtaaatgta tagcaacatt
     2881 attaacgtct ctaacagctt caagaaacga ttccggaagt gttgggtggg catggattgt
     2941 gtcctgcacg tcttcaattt tcatattcat cctaaccgca gggcaggtga taaaatggat
     3001 accacaacac gctggctttc aatgcttggt gtcggacact gtgctttggg atttcgttaa
     3061 tcgtaccgag aattccgata cgaactatta ttgtcttttt cataattggc gtgccgctag
     3121 ttgcactgtg gtgatatgtt gatacgaaat caaagcaaca gatgaatata accagcaagg
     3181 acagaaaaac gtcaaggatt tgcgcttgtg catgatccaa tgctttactt tcatctacaa
     3241 acaacgatat cccactcatg gccttaaagt gtaatctatt aactactgat tcctcaccat
     3301 aatgattgtt aaagtagact ctgagtattt gtttagattt ctcaagctcg ttataattga
     3361 atgaaaaatt aaccagtcat tgtcttactg aaaggctgcg agcacgtata tctcgaaaaa
     3421 aataatggag agaatatgtc tttactcttg acttgcgcta gatcagcggt gtatgtcggc
     3481 actattgtcg ccagtattta ggcaaaatga gctattacta cagacattgg cttggcttat
     3541 gtcttgactg acttttatgc tgttgctgtt attattatgg ttgtggtgtc cgtatgccaa
     3601 agcctgttgt ggtacagcga atgctactgc agtcatagct actgttgcta ttatcgccgc
     3661 aattgctaaa gttgtgttct tgtgattcat ttgttctcag aggactatct tccattatag
     3721 tatatttgaa ctgcttcaca acaactgcta ataatacagt agaaattgtc gatatattta
     3781 aactctgata aattcattag aaagaataaa gttctaatgt tgcttgatct attaaagaga
     3841 atgatactct aagcaactca actcagatat cttttatcta ttattaacaa cgcaagcaga
     3901 agctcgggtc ttgaatgaaa cgaacaatct tttttcagca ggcgtttata attatttgcg
     3961 acaggacaat aatatcatag cttcctctca tgttgtgtta gattaattcc atagagccgt
     4021 ctttcctgct ccactgaagt tatatctacc cagaaatgaa gctgccataa aaccagccac
     4081 ggttacaata attattttgt taaaagtaga tctttttatg gagacgttgt tgtctacgcc
     4141 tcacggtcta gcagcttgct aatttcccaa atctcacaca atctctcaaa ttcctagatg
     4201 taaatcggca attattcggc cgatttcatg gtctgtattt aacaaacgca gtagcagtgt
     4261 ttcacatgtt tacaggtata tcgatgaaaa gaccgattca gctgaggctg agcattaaac
     4321 aagaactcat caggtcaggc tgccagtttc ttttctgcag ctgctaatct aataggttag
     4381 agatggcaat tatagatatt gtatcagcgg attacttaag taagaaatag tattctcatc
     4441 tatcatgagc tcaactccag gatgtaacgg tcaggctatg ttattgactt cgagatctct
     4501 gcttgtagta atctttttgg cttggattct attgggccct cttcttttaa aatttccgtc
     4561 taactcttat gctggatcat caatgcgtcc acaaaccccc gcacctaaat tacaatccga
     4621 gaaaatgtac tcaatacaag caccaacacc atctcgtatt caaataacaa atccaaaaaa
     4681 cggcagtaca ataaccaatc cttatccaat cctaaggggg actgctgaca cacgctataa
     4741 tcatgtcgaa gtatttgtaa acaatcgaac agattatcaa acacatgcgg ccaaagaaga
     4801 tggcaagggc cactggtctc tggatatgtc cggcttatta cttcctggac ggaacaatat
     4861 aacagcaatt gctacgttct atcatattag cttaaaacca tcgctctaca gcaacagtac
     4921 tattccatcg ttctacttac catcaaataa tactgaaggc tgggtgtatt ctaaaattca
     4981 tcttgatttg gttgtaccac ccccagttat atcaagtcca gctgagggag ataagattca
     5041 gattggtggg gtcacgcgac ccatcataac agggacggta gatcaacatt atgtttatgt
     5101 tggtttgagt gtaaatatgc ttaaaacaaa tacgagtgtc agccaaacca tcggtgaata
     5161 ttataatgac agtgttcctc aaaatggcac aggctactgg tcagtcaaat tacccggtgg
     5221 tgatttatta aggcctggca tgcagaagat aacagtctgg attccattcg tgaacttaat
     5281 gggcgaacca gttgtcaaga tattcggcaa gcccattttt gttggattta tatgcggcga
     5341 tagaattatt gtcatgcatc ctcatttctg ctcccattaa aaatgcctgt tgaagtgtgt
     5401 gcaaagtaca aattttggta aataaccatt aacgagtata cacggactat gaggacttgt
     5461 gcgaacatgg catatctcga atatctttat ggactcttga cagtcctaaa taacattaat
     5521 ccactgatat aatcctaatt gtcgaggagg gcatttgtat tgaactgcaa cttggaacgt
     5581 gatcgaaaga ggaagggtcc tcatatgaaa ttacaacccc cacaggatca acagaactct
     5641 acatgccatg gcgtaaattc caagctctga atatgttgtt atcccctgtt tacggtaaac
     5701 cgagcgctac tattgatcct tcaaatccct tgggtgcttg aattttacct gtaggaacat
     5761 agagc
//
LOCUS       DAOR01000246            3811 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_45901, whole genome shotgun sequence.
ACCESSION   DAOR01000246 DAOR01000000
VERSION     DAOR01000246.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_45901"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1766..2003
                     /estimated_length=238
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          974 a          727 c          641 g         1231 t
ORIGIN      
        1 ttaacataca gaggagatga aatataccca atgaataata gtacaaagga acaaacctac
       61 agagcagata aaatatctcc aacacttatg gatgataagc ctattagacc tttatcctca
      121 aatattagtt tagtgagaag gaatgatact tacaagtcaa ttgacagttt ggccaatgag
      181 attttggatg cttcagctga ttgggtagag aatcgtaata actgtctata aagttagtac
      241 ggtttttgat tactctattt tcacaaatat catactcgct attttcttaa ttattcatat
      301 agtctggtct atcgattcct ttggttgaat ctgatttctc aatgctttgt gtacagcatt
      361 tcattttaga ctcacccgaa tctgtatctg ctaaggtatt cttttgagag gtcctctcct
      421 ttttgtgtga ttgttataag tatcgggacc tattttttta ataatcacta gatccttatc
      481 agacagcaga gtatatactc tgtctgcaat cctagtcctg tcataacgac tgagttgacg
      541 ataaggtcca aatttcttca atgactctgc aatcttgccc aatccgtaca gagtaaaata
      601 aactgaacta tccatttctt ttccatatga tctaaacatc ggggtgtttt tggcaatata
      661 gactaataac aagtatctag tgattcttgg aaggtcagga tccccgtcgt taaactgcaa
      721 agtcatagat tagtgggtca agtttgagat aaacatttac caaaatacat tgtttatggc
      781 taagtctttc gtgaacatta acgtatgtaa gactcgagtg aaggtgtgaa agcagtgaag
      841 gttctatagg aaataggtag gattattttt gattatgatt tttgaattgg agaattataa
      901 caggaaagat atttggatgt aggccattat catgattata attcatagga gagtaatcgg
      961 tggtagtgtc ttcgtatgat tccagcatac accctttaat ttccttcgct gttttgcatt
     1021 ggatttcatt atattattct atttttaaac atgggccgcc tgtagtctaa tcgactattc
     1081 ctccacagtt tagaaatgag tagactgacc aaatcaattt gaaggcagac ctattgtaaa
     1141 caaattccaa acctcgcctt gtatatcaaa tcaaggattt ttttaaacca caacaatctc
     1201 caattcatca taagcgtctt ttactgagag ttcgactaat gtatcctcat aaattaataa
     1261 ccagtctctc attccaatga ctggcaactt tctcaccaga accttcttag tctgtattag
     1321 ttttgtcaat agataagcat aacgtggact acctcctcta tcttgatctt taattttgaa
     1381 aaggctttcc tgcagttcta ttccaggcct accgccctgt atattgatcg taaccttgtc
     1441 gccatatcta ttttacactt tggctgtaac ataactctgt ccaatttcaa atcgttcaat
     1501 gttcaacaat actctagatt tgatgactgt tgattctggt atctcgtcaa acaatgcttc
     1561 agaaatatca tcaaagaaca acttatgatg tgatgtctaa acggatatct tttatttaag
     1621 atccgatcta taaactctta ttcctcaaat tttaatttgt accagaaact ttagtgtata
     1681 tgatcctttc catccttacc tcaatcttga catcatacac aggacttttg ttactgtaag
     1741 gtactgttat gattgcatta tctaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnntactgtt atgattgcat tatctaattg ttgttcgatt
     2041 aacagcatta gtgaatttcg tttgtaaatc aatgtctgct ttatctattc aacatgtttt
     2101 agtcgcgtgc aatacttgaa cgtcaccgac aatatttatt cgattttttt taccgacata
     2161 ccacttagtc tcctaaatct ttttttagaa tatcgtcgtt actctggttt actctaatat
     2221 tactccaaat accggaaatc ttctaagaaa gcaaatgaga cgtatgaccc atatcgtgtc
     2281 accgccgctg caaggcattg cagaggaatt tcttaacata tgtcttatgt agtcggttat
     2341 cctatcagcc ctaacgacta catataagag aatcatattg cttcggctgc ctccaataga
     2401 tgttctatct ctcatttctg ttcgcttcgg tgcctggctg agcaggatta gcatcattcc
     2461 atagattctc gaatgcttcg cgataaacat caaaaagttc catctgacct tttcgatcta
     2521 tcttgaatgt ctcgcgttcg tcttgtggcg tcttgtatgg gtatggttct atctgcatag
     2581 attcatccat tattactaag ctcattgtcg gaatttgcct atgccatctg atttcgagat
     2641 tctgtgcgtc gctacttccc aaagtgctac gggtctggta tatcatgtta acacaggatt
     2701 gaattcgatt tttagtagtg gttgaaacca ccaccgtttc gatatctcgc atgaacggtg
     2761 tttcaggatg gcatagtaaa atcctcactt ttttaccatg ctttgcagcg gtttcgatac
     2821 tggttctaac gtgattaagg ctctccactg tgataccctg cattataatt tcccgttttg
     2881 tttgcttgaa cagctcactc cacgatggca agtcgtctcg gtcatagaca accactgcgg
     2941 gttcttgagt cgtcgacatc atctcggtct ttgacgccgt cgtcttacga tagaacggcg
     3001 agcgcacacc gattacatta ctgatacgag ggaggaaata gacaaacact gccccggaga
     3061 agattgttat tatggaaatt attgtccaga gtgtagacaa atgaatggat gtcaataata
     3121 gtgtcacaat caaaccgatc gctccaatga caatagcaaa ttttgattcc ctactatgcg
     3181 gttccaataa atctgcttca tttttgtgct cgcttaaacg ttgctgccat cctaatcaaa
     3241 ttctgcattt tgttttctcc tagctgcaat tttataccag cattctctgc aggcgttttc
     3301 tttaatgagg agtgttctct tagaaaattg taattaatcc taattgcttc aatgatttgt
     3361 tgcgcggtcg cctttgtctg cattccgcgc ttgaccttct cacgctctcg gatggttcca
     3421 tgcaatcgtt ctactttgga atttaagcct tcgtgtctga ccgaaacact tctaacgttc
     3481 ttcacgcgcg gttgtttaag gctgaaatac tctttctgaa atgcttcatc gtaagaccgc
     3541 aatccatcat gaattattgc tttcggcccg tttgctcttt tttttgaatc ctgaaatact
     3601 accctagcgt ctgtgacctc tctgcgaggg agtcgctacg aagttgtatg tgacggattt
     3661 tcctggttca atgaatccag cagctccaga aattccatcc attgtacctg gatgcattga
     3721 atccatatgt aacggatgag gcgattgtga gtcgttagtg acacttactg tgatttgaac
     3781 atggtcacct tgcgtaaatc tcatggttgg t
//
LOCUS       DAOR01000247            5588 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_56813, whole genome shotgun sequence.
ACCESSION   DAOR01000247 DAOR01000000
VERSION     DAOR01000247.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_56813"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1762 a         1058 c         1129 g         1639 t
ORIGIN      
        1 aggtggaggt ctcactaaac ctgctgcgaa agcgatatta aaagagataa aatcaaatcc
       61 gctccatagg atacttgttg ccgttgttca agatggcaga aaccaaggtt tgataatagg
      121 aacaacaacg ctccttgtag aaccaaaatt cattttcggt ggaggacgag tagcacatat
      181 cgaggatgta gcagtgaggg ctgagtttca gaggaaaggg attggattca agcttgtaaa
      241 atacgctacc gagcaagcag cgataatgcg atgcgtaaga acagtactcg attgttcaga
      301 tgagaatatt cctttctacg aaaacattgg ttattcgtat cacggtaata gtatgaaaat
      361 tcagcacata cacgattaat tcaatacaaa cgattatttc aggatcgtag aaaattaaat
      421 cagatagctt gagcttggcg gccatcttct aacttggagc agaaataatt tcaaagttag
      481 aacataacgt catggtatca tagcaacgtg ggcattcgat ttcaaattgc ggttctatcc
      541 ttgtattatt accatttact actatcacgt tgtctgagct aggaaatgaa acttaccatt
      601 tatgagaagt cacagttcgt actacagggg tacatatgcc actatatcgg gtcttttagt
      661 tgggtatgcc gtgacataaa caccactcag catcttgcct cgtagagaag tcctaaacta
      721 tgtgccgttt tatttgggtg ctcataaatg gttgaacatc aaacgtaaaa gtttatacgt
      781 attaagacct gatgacaaaa ttgcatctgg cgagctagct ataattgaga ttatttaatg
      841 actacgctga tatagaacat aaggttaaaa aagaacctgt taagcacgaa ttatttgcaa
      901 gcatagctat accctttcac gagggatatg atctacttgc taggactatt gcaagcctga
      961 ctcaacaaac ttactcaaaa gatcgatttg aggtcattat cgcggctgat gctaatcatg
     1021 ctgatgcaaa attgatcgct gagctgtata aaggagaact agacatcaaa ttagtaactc
     1081 tttctaataa cggctttggg ccagcaagct ctagaaatgc tgggatagat gtcgcgaagg
     1141 gtgatgttat tgtctctctc gattcggaca tgatatgtcc tcgtagattc ctagagtcac
     1201 acttgagctg gtttcataaa tcagccaaaa ttgcgacgat tgggcttcga aaatttgtgg
     1261 atgcaagcac aattcaacct gaagacattg ttcgcaactt ccacctgata tcgtcacttc
     1321 cggcaatctg tagtatttca aatacggtgg taggcagtcg tagagacaag agaattcctg
     1381 aacttacaca gttaaggagt caccccttcc caagcaattg ttttcatggc ggtaatgtgg
     1441 catattggcg tgacgatgct ctcgatatcg gactatggga tgagggattc aatgggaatt
     1501 atggctatga ggatattgaa ttcggacagc gcctgtttga aaatggtaca aagctagttt
     1561 tcgaaaatgg tgctacagca taccatcaag agaataattt cgtttcacct tatgaacgac
     1621 agaagggcct gtcaataaat agaataaaac tttatgagcg ttatcctgcg cttgcaagat
     1681 tccgaaaaga tattctatgg gcatgaggat attggtcgag cgtatgaaag tgtggctaac
     1741 ggaagagtgc agtttcgtgc agtaatatag gagtaaataa aactgcggaa attataaaga
     1801 ctgttgggcg attctgctcg gattagtatt agcgtcgacc acacacacaa tatgacataa
     1861 tttctcagag tatagaaccg actgcaattt cattttagtc atctgtggat attaattgtg
     1921 aaaacacaaa ctgcactagt ggagtaatac cacagcatgt cgcttgattg tcaactacaa
     1981 cttttactaa ctgttaccat ttgtctttat tgtatttatt gcattagtcg gctctgtagc
     2041 aacaagtgct tttgtgaaaa ctcttgttag ctggctgatt tattagtttt gtattaagag
     2101 ttacgaagat gttattttta acgctccctt ttgctattaa taccgggtta taggtactta
     2161 ctataatgct ttttcttaca atgggttcta tccattcctt gcattgactg acagcacaat
     2221 atgggataaa cggtttgata aatggcagga atcgttatga actgattaac cggtctacaa
     2281 ataaaaaaaa gagaaagtta tcagctttca aaatatggtt tatatctttc gactagatcc
     2341 gaaaggggta accttttgcc tcctactatc ttaatgtcaa cattagactt tccattctta
     2401 atgtctataa tattgtaaga gcattcaaaa aatcctctaa atcgagttga cgacgctgtt
     2461 ccagcatatg caatttccaa agcacctagt ttccaaaccc aaggtctatg tttatgacca
     2521 caaagaacta aattgacttt agattgaaag catgccctta aagtgtcacc agcatcaaat
     2581 actacgttct tgtcggtacc tgtatccgga atccctatca gatggtggtg cattgcaata
     2641 atctttgttt tgttgtcata tttactcatg accttttcca tccacaagtt ctgccgatat
     2701 cccacctctc cttcgtctct gtcaggacga gccgtgctaa tagtcagcaa tactacatca
     2761 tcatcaaact catagatccc tttcggtgac ggaaataatt tcttgagaag tagatagcca
     2821 gtatgacgat aatcatgatt tcctgctaaa actattatgt tagagcagtt aaacctgcta
     2881 atttccgcac gggcacgttc aaactgatat agtaacccct catcagtgag atcgcctgta
     2941 atgattatcg catccggctt taacttattg ttaacctcat caactaagat gtcaaagaca
     3001 tcttgtttga aataactttc gccaatatga atatctgata cttgaactat ttgcattttt
     3061 gttcatccac ctatacgaca cacactggct cttaatacta ttgttttttt gcttcatggg
     3121 cgattaaatt gatagattca tagcatgtga tccagatcaa gcgctcgacg aacaagacaa
     3181 gtcgcccttg gaaatttacc taattacaac gatcacgata tgatcaatat aattacaaag
     3241 gaaaattttg agaccccata cgagtgattg ctagaattag ctttacaatt ttcgccaatc
     3301 tgtctctaca ttaaggttgg taacttcggc aataccagca agatttgaca gccaatgcat
     3361 aaaagcaaat gtgtcctcat ctaaggtttc gcatttgttt gacttatttt cacgtttata
     3421 ttggctacga ctatctaaat catcgtctga ctgttcgcgg ttactattgt tttccataag
     3481 gaatttccct tctgaatcca actgcagtta ttgagaataa caatactcat gtatgtaata
     3541 gcatctatga ggattgtgat tcattacttt atctgaggtt atgctaagaa atgaagcctt
     3601 aaaacttgtc tagtattgaa cagcagttgt gtttatatgg ggtttcatgg gctactacgt
     3661 caactgtgga tataattatt aatattgctg ctttagaaac cccaagtgtc aaaaatgtta
     3721 attgttatgg caagtttaga agaatagctc catgagaaac cctttcaaga aaactaacgc
     3781 taatggaatc cctgatagag aaccgtttaa gtataaagga ccaagggcct caaactcggc
     3841 cctttcttct aacaaattaa aaattgtagc agggataatc acgtttgttg tgatcattgt
     3901 cattatgttc gaatcagtag tagtagtaga agcaggacat aggggagttg ttctctatgt
     3961 gggtgcagtt gaaaaccgtg tattaggaga agggctacac tttatcattc catttgctga
     4021 acaggtagta ccactggaag tacgtacttt gaaattccag gccgacgcct cggctgcctc
     4081 taatgatctg caagaggtac agacaaccat agcattgaac tatcatatta gtccgagtca
     4141 ggcaaacatc atttatcagc agctaggtgc cgattacgca gatagaataa ttgcaccaac
     4201 tatacaggaa tcagttaaag caagtgttgc gaaattcaat gctgaagaat taattactaa
     4261 gagagctact gcaaaagctg tgatagctca gactatcggt aatacactat cagcaaggaa
     4321 tatagttgtc gagacagtat tcataacaga ctttaaattc tcacaagcat ttgctaatca
     4381 ggtagaatca aaggtagtcg ctttccaaaa atttctgaca gaacaaaata atctaaaagg
     4441 tatacaagta attgcaaacc agacagttgt tcaggctcag gcatcagcta gggcaaatgt
     4501 agccagagct agcggtgaat ctcaggcaat ccaggtcatt acggtacaat taaaacaaag
     4561 tccacaatat cttcagtggc tatcaattaa caggtggaat ggacaaatgc cgtatgctct
     4621 gggtagtggt gcatttcctt ttttccaatt accggtagca gcaccgccac aaaaccaaac
     4681 taaatgataa tctcgcataa tcgtccaggt gccataactt aggataaaaa ttgaagattc
     4741 acacatgtga gaatgagtaa tcgcagatgc aatattatga tgcttaagca ttgcgctcgt
     4801 cggatccacg ccttgcaaat ataagatggc agtagttgtg gtagtcgctg ccttggtaag
     4861 tttcgctttc gcccttagac agtaaactat aaagtaaata gcatgctatt agaaatgtac
     4921 ctagctatga gcgaatttgc cttcttcaga tttagaagta ataatatgct gaccaaggag
     4981 gaggaagagc ataaagagga gattatccaa ttaacggagg aaatattgca ggccaaaggt
     5041 atccaatgtg atgctagtat ctcaaaaatc tctgaggacg aattaataga aattctcgag
     5101 aaagtcagag agcttcgtag aaggaaaaag aaaaaacaat caggtactga agttattaag
     5161 gaagaggacg tctatataca caacaggtga caaaatggaa accacaacac gctggctttc
     5221 aatgctcggt ataggtactt tgtgctttgg gatttcgtta ttcgtactga gaattccgat
     5281 acgaaccatt atcgtctttt tcattattgg cgtaccgcta gttgcactat ggttatatgt
     5341 tgacgctaga tcaaagcaac agaagaagat cagttctgaa accaccaccg caacacaagt
     5401 gtgtatatgt acgatttgta aacatgacga agcaagggcc tgtttccagg aaaactgtgc
     5461 atgctgcctt ctgacaaaag gaaatactgt tataggacat tccattaatc cgttgcagta
     5521 gaacctcgta ttttggattt gcactcgcag gctttccttt atcctctcgg aggataaaag
     5581 acgatcca
//
LOCUS       DAOR01000248           10095 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_13258, whole genome shotgun sequence.
ACCESSION   DAOR01000248 DAOR01000000
VERSION     DAOR01000248.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_13258"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2784..3023
                     /estimated_length=240
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3241 a         1829 c         1999 g         2786 t
ORIGIN      
        1 ctgtgagttg agctacaaac tacaacccta tatatccctc aaatgtattt aaaggttcaa
       61 aattctccac cgatactgtc accgaatgtc tgaccactga ttgcgtcgac tgcacaggat
      121 cgtatagcaa cgaaattcta ggccaccgtt ttgtatgtag atgtcggtgt cacaaccaca
      181 cccatagcga tgaccatgat ggggagaaaa tccatgtacg tcgaagacgt gtgattcctg
      241 ttaaagatca agaaaaaaag cgtgaatatt ataattatgt ttgatgatga aagtaataca
      301 agtctgcagc gtatcgctca ttcaagttca agttcaaact caaatagaac gcaactggga
      361 acagaacatc tggactgtgc taagccggaa gcagcaatca caccatggac tactgcgaaa
      421 gcgatagacg agctgatttc ggaacttgct ctggcaaaca tctacatcga aaatcaagaa
      481 tgcggatcta tccgcatcat tcctaagtac tattctcggg cccaagagat catgtatcgt
      541 attccagtct acaggccgga cccacctata tttgaggtcg acgtaaagga tctttcttgc
      601 caattacaaa gcgagttagc tggcctcaaa aaatcggatc ataggatgtt cgactatgtt
      661 ttaaagatag cagttatgca tattttcgag aacatcagat ttgattatgc tcaccaatct
      721 gtaatgccag acctaagaat acggtttgtg gataagtagg gaagaagaga tgaatccggc
      781 agaagataag aagacccttc tagaggaaaa cgaggactat gccccaagta cgttaccatc
      841 atcggttaac gtctgtactg tttctgtcgc cataaggatg cattcggggg atgtcgaggt
      901 cagaggtatg atatgcggac taggtgaact cgaaaaaatg ataactaacg tgtttttcga
      961 atgtggaaat tgcggggcga tgaatgagct acaaacctat tcaagaccaa gatttccata
     1021 tgaggtggac tatactaatt ctacaagggt aacaagatgt attaattgta acaaaaaatt
     1081 cggcaacaag tacgatgtca aggtcaaaaa tgcggtcaat atcgaattac aagaccttga
     1141 caattttaat gatctagaga gattatccgt ggtactgttt gatgatgata caaagaatat
     1201 atcgattggc gagcaggttg ttatcgtagg attaattgaa aagataagaa agaggggcaa
     1261 aatactccca tttctatttg ctacttctat cgagtacgag aatcgggaag aactagtcat
     1321 gactgagcag gatatgaagg agatacaaac atttgcaaac aactgcggaa atcggaacgg
     1381 gaagaacgta attgacgcac tcgtttttaa attcgcaccc tctgtagttg gatacgaaca
     1441 tgtcaaaaag gggttgttgc tttgtgctgc aaatactggt aatgaccacc tatcgaagaa
     1501 acttagaata aattcattat tgatcggtga gaccggactt gccaaatcta tgctgctaag
     1561 agaatcagtt gcactcgtcc caaatagtcg atatacaagt acattaaatt cctcagttaa
     1621 gagtctgatc gccatcatct cgaaagagga tgataagaat atgatgagat taggaccagt
     1681 cccaattgcc agcggtgcaa tttgtgctat taatgaaata ggccgaatgg cgtacgagga
     1741 tcagggggca ttcttagatg ccatgcaaga aggtaaaatt cccttttcaa aatacgggtt
     1801 taatatgact cttaacggct ctgcaacctt tatcatgtct gcaaatccaa cacatgattc
     1861 gacatggaga catacagaga aaatagatct tagcgagatc cctgttataa tgccattatt
     1921 ggaccgtatt gatctgatct tcatattcag aattactcgt gacagagagg cgatcgcaac
     1981 atacgcgttc aagaaaaccg aatcctacat gctaaaggat tccatagcag aacagcagga
     2041 agcaaataaa gaatccgatt ttttgaaaaa atacattctt tataccaaga gattcaatcc
     2101 aaggctcacg caggagacag gaagtatgtt gtgcgaatac tatatcgatg tcgcaaccag
     2161 gttcggttcg ccaagggtac ttgatgcatt attcagaata agttatgcta ttgccagact
     2221 aaaacaaaag gaagttattg actctgagga tgcgaaagag gccattgaat tttacaacgt
     2281 tcaattgcaa caacaatcgg aagcagtcat aattccgaga gatccacagg agttagcctc
     2341 agaagaaatc ataaatactc taaggaactc agaatgtccc tatgatttta tccaacttgt
     2401 caaagaggta tgtaggaaga acgaagcagt ttcacgctat atcggtagtg atttttcggt
     2461 tcagaccaac aagaaattca gaaatcttcg ggataaattc aaggaaggaa aggaggctaa
     2521 tatcgcgatt gttaatcaac gaccattggt acttgcatgg cgttcctact cgttatcgtc
     2581 ttcgtcctat tatgatcata gcgataacac taatctgggt gatgatttgc gatacacaat
     2641 aattcccgta attaacaata tttgttactc tcaagctctt cttagaaaca aaatcaggca
     2701 ccagagtagc caacataacg aggaacagaa aatgcctgta gaatccaata gtggaattct
     2761 tcacccaact gacccaactg accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnacccaac tgacccaact gaccaaggga aaacagagat
     3061 gatatcaaaa aataatattc gaaatcacaa tgcatatggt tctggcacta aattgatagt
     3121 aagtatagtc gtggctgtcg tggttgttat aatgattgct ttcgcattaa ccaccataca
     3181 gcaagataga agtgcaaagg caactcaatg tgcacagatg ttgggacaga taaatcaaga
     3241 gacaagtagc tggtgggcaa ggaactttga tacaaatcag attaaccaac aggtagatca
     3301 atacaacggc atgtgtggca gtggcccaac gaattatgca ggtaagtaag ctactcaaag
     3361 gaatactgca tgacattaaa gaggagataa aagacggatt ctcaaatttc aattggccga
     3421 tctggtagag aatagaatga cattgagaat tgaatcagga caacgggttt atgatgcgat
     3481 gactgactct actcgaatct gtgctcaatg tatacatgtt gaagtgtgta caatttttag
     3541 gggtgtaaat agggcactag aatgggactt tcctaatatc aaacgaccat tcgaaacacc
     3601 tgacatagca aagatatgta atcatttcac aagggaaact ggcatatagt gagcacggaa
     3661 ccatgataga aacaaagttc ataatttgag ccgagaaatg aaactcatac aaatttactt
     3721 agacgatgaa actcatacaa cactcgtcaa gaactatact attactctag ctgctgctga
     3781 agaatcattt acatttgagg actacatcgt ttacttgcta caagctgtac ctccatgtcc
     3841 agctactcac gctgttcaac catcgggaat caaagaaaaa acaattaaag tcaagcagaa
     3901 aatattgagt tttagaggta aagagtacct tttgcaaaac tctattgtag acctagaatg
     3961 caacgagtgt ggccggctat tccgttgtcc gggagtttat cgccagcgaa tctacaaaga
     4021 cggaacgagc aaggaagttg gtaggcaaaa aataccggaa tggcattgtc ctgatcaccc
     4081 taatgctata gtaaacgcgc ttgaaactga aactctgaag gaggatcctg atctgaaatg
     4141 aaagctgcat tgtgtgataa tagaaaaaat cttttattca tcgcaagcgt ggaaatgcaa
     4201 tgcattaatg gcacacctac cggcctctta ttgtgcagga tataaacttg gatgtcccag
     4261 tactactgac aagtggatag aatatgaaca ataaaattct gtctacattt ttaaccttct
     4321 ttggtgtttt tctgagtctt gcgagtccct tatttcatta catattttgc agttgttgta
     4381 gtcgtgatat ttacctatca tttcttagta tgcctgctgc tcctccgtct atctactatt
     4441 cctagaagtg tcatcttgag actatttaca attgaggatg ttacaaacaa ggattttacc
     4501 gcattttagt tattcatatg atatggtatt acctttaggg atcttcgctt tccatgtaat
     4561 tccaaataaa tatattccat ccctccaagt ctcctacctg acccggtaaa ttaacagaac
     4621 ctgttagtac aatctataaa taacatagga agaatttgag tatttgaaat gtcttctact
     4681 accagtccgt cttatagatg tgaagtatgt ggtacatcat ttacctcatt acaagaactt
     4741 gatcagcaca agcgacaaca ccatcagaaa taattctaca gattgatatc gttcccaagt
     4801 aatagttatc accaaactca tcaatctgtc cgtgattgat tgatccaggc tcttaactgt
     4861 ggactaccag gagtcggaaa aaccacgtta gcaagagatc atgcccactt aattaatgcc
     4921 gtcaatatat ttcgtaatat gttaggatta ccagaaacca aatggaagag aagtgagaag
     4981 gacaggactc acagggattc ccctgcgagt gattcggacc aacatacgcc ttcctatttc
     5041 ttagcctatc aaccgtctat cgaaaggaat ggactatttc tttactgaag atctctattt
     5101 gttcaatgta tcttactggt aatagcagca attgccgaca atgtagttaa tgcgtcaagc
     5161 caacttgtgg tcctaaacac aatatggtca tttacgccta aataatcata atcgagatat
     5221 tctgcttttt ttgcaaaaga aagggttctg ttgaatcatc ttcgtgttta aaatcgggtt
     5281 gatttcgtgt tggtgaattg ataatgggga tagatacggt caactcactt tttgagcata
     5341 tctaataacc tttcaaatat ctcctttccc ttaggcgtaa tagagtaata tttaactgct
     5401 tgtggtctag aatctttgct taagccaaat tcacttctga ctaactttgc cttttctaat
     5461 gcatcaagat gaaatgaaat aaccctacgt tgtattttca aagttttttc taactgagta
     5521 ggatacattt ttttttcata ggttaaaaga ttaaggattt gaagcctagt ggattgagcg
     5581 gctacatgaa ttaactcgat atccggatta tctgtaggga tttttatgga tgacactgct
     5641 ctttgagttg acatatacta tatggaatat taactattct actacttagc ttttgtgata
     5701 aaggcaaggc ttttatacta ctagtacatt atatattctg atagaacata taatgttcgt
     5761 ggtggtaagt tgaggttgtt accgtccaac aattcggaca tgcggatgaa gaggggaaca
     5821 gaaatacttc aaagcaatgg caaaagcata acagagaacg aagatggaag tttctctgtt
     5881 ccttctcaga cgcacctcca tagtttatga agttagacta tttgatacta tttgggtttg
     5941 tactagtcct gattttgaaa ccagagagat agaagcatgc aagcatatcc attgtgtaaa
     6001 gttctggatt gccgccaata catatttgca agataaacca aaaccaaaag tcttagttga
     6061 aaatacgata acatgtaaaa gatgtgcttc agcaggtaaa caaactttgt tctgcaagga
     6121 ttgcaactat cgatttattg aagatacgct actatagagg gtaaggttta cacctgaagc
     6181 tattacgttg acactagacc tttacttttc gggactatcg cttagaaaga tagcacgcaa
     6241 cgttaatgac cactttgata ttcaagttga ttttactact atctacaatt ggataaagag
     6301 atacattccc ctaatctcgc agtatgtaaa ctcactaact ccacaactat ctgacacatg
     6361 gcatgcagat gaattgtttg taaagatgca tggtggacag acatacaaag gaaaatcaac
     6421 cttgcattct tgtggaatgt aatggatagg aaaacaagat tcctgttagc ttccaaggtt
     6481 tcggaggcta gagatatcaa tggtgcaagt aacagtgttc aaagaagcag taaaaaatgc
     6541 taatggtcag aatcccagag aaatccatac agacgcatta agggcatatc gagagggtat
     6601 atcacagaat ttcaaggatg ttgatcatat tcctaaatgc ggcattaaca aacctcacgc
     6661 agacaataac aggattgaaa gattgaatgg aacaataaga gaaagaacta aagtcgcaag
     6721 agcatgtaag aaacataact caccgcttgc tgaagggcaa aggatacagt ataactttgt
     6781 gaaaccacat caagcgttag aaggacaaac cccagcacag agagcaggaa tagagattaa
     6841 aggaaaaaac aaatggatgg aaatgttgaa gaattttagc aaaacttcat aaaaaaagtc
     6901 tttatatata ccaaggtatt gggttaagta gacgacaata cattgagtaa tcaaaagaga
     6961 cttagattga agactcttat agaagaaaga tgtatcaagt ttggccctta tacactatct
     7021 agtggtgaga aaagcaccta ttattatgat ttaaaaagta ttttattaga tggaaaagcc
     7081 ttaatcttac ttggagacct attactagaa gaactgtcaa aattcagtcc aagtccaaag
     7141 tccgtaggcg gtttagaagc tggtgcgata tcaatagttt ctgcagttac tattaggagt
     7201 tacaataaat atactggggg tgttaaagga ttttttgtaa ggaaggaacc taaaacacat
     7261 ggatcccaga agaaaattga aggtaaccta gaaacgccag ttgtaatatt agatgatgtt
     7321 atgactaagg gagggagcat caagcaagct atagatgctg taaatgtcga aggatttaat
     7381 gtggcaggtg tggtctgcgt aatagacaga gaagatccag caaacctctt aaagcaaaat
     7441 aacataaaat attctgcatt gtttacacat tccgatttta aggaattcat aaaaagaaaa
     7501 caacaagata caaaaattca ttctgtataa ataaacatca acgtcacttt ttttattagg
     7561 cgaacgattc gaccaacatt atttcaacaa cattcttcaa atacgctgat tcaacagaac
     7621 caaagaaagt atttcaactc tcgaatgatc tgcttctcca ttaaatctta taattggtaa
     7681 atgcagtccg aagccaatag tttgcttcat tcggatacat agtaactaat gtatcccatc
     7741 aaaacctgaa ggggaaattt caaagggccg gaacacgaag cagtgcaatt gctgtttatt
     7801 ggcaactttg gaagatacga gaaggaaaag tttttttgaa tttatcacta ttccatgaac
     7861 aatgtttagg aatttcctag agaaaatatt acggattatg atatagacaa agtacgacaa
     7921 ttaaataaat acgtaagata cgctagacta agagctagga ctagaaccct agcctgctaa
     7981 gactgcgggg gctcttctga atcttcactt ccgtcaaccg tttttgcttt gcccgacctc
     8041 gatgatatag ccatcagggt cacgcatgta tcagcggctc tcgaaacgat ggtcctttgg
     8101 ctcggtgagg aattcggccc ctctctttcg ccactcctcg taacaggatc gaatgtctgc
     8161 gacacgaata ttcatgaagt tgctggcctc attagggtac tgaggcgtgc gtagggtcac
     8221 ggttggctta tcatcggttg ggccgccacc tacgtttata atcaaccagc tgttagcaat
     8281 ttggatgatg gtgggttcgc cttctcggac aacattccct ccaagaatcc gaagataaaa
     8341 ctccgccgat cgtttcacat cagctacggt cagaaagtgt gtgaggataa acccttcttt
     8401 gggaggcaat tgatagcctc ctcttttgat cataggagat tcttcttctt tctggtcgtt
     8461 aatattttgc atctattatc gacttttctt tagtagtacg agttattatc tttggctagt
     8521 cagaattcag cacacgatct gtctatgctg cgttccgctg ctcatgcatt aaaatactgg
     8581 ttacctgatg tatttcagcc catttatcat gctaccataa tttgatccct cttgaacgtt
     8641 ataagtatga attgggatat tctcctctta gatatgtttt gattcctttc ttatctccaa
     8701 ataatctcgt cgctataata acaatctaat cccctgtcat gccctatagg gaggcattcc
     8761 actacacgaa ttaatcccgc agactttacc ggtatacgct ggtgtaatta tttgtgtttc
     8821 ttgatataca tacttagggc tgatttccgg aataaagacc cgtaaaacgc ctacatctaa
     8881 cttataatgg tgtcccagtg ggacattaac gtcataatgg ttttgcgttg cggctgcggg
     8941 gaatattgta caggagtatt agcgctagta ctactcggca aaggcatgcg cttgtttgac
     9001 catttaggca ctgagcacct tgaattggaa agaactgggt tcatagtccc ctggcgtaac
     9061 ccatcttaga ttcagcgtcg taaagcaagt tttctagtag gagtgtggag tggtagtaga
     9121 attaattatg tcttacacat gatcttattg attcgttgaa gtggcagaaa atgagaaaat
     9181 cagttggtta tcgagtcagt gtataagggt agaaggacaa aggttatgtt attccacatg
     9241 ggatgcataa cacagtgtac agtgacgaga acataaagtc acgaaatgga gagaaaccta
     9301 gtcatagcca gattcatggt tactcacact tagttgactt acgcgattgt tatccatttg
     9361 tgaaatgggc tggtggcaag agccagatat tatctaagtt agattcaatg attccatcta
     9421 aattcagtcg atattttgag ccatttcttg gaggtggcgc gatgttcttt catttgatat
     9481 ctgatagaaa catgaggttt actgcctatc tttccgatat aaactgggaa ttaattacgg
     9541 cgtataaggt tgtgaagaat aacgtagggg aattaatcga acttctcaaa agacatgaaa
     9601 gagaatataa cagaaatcct tctaaatatt actacaaatt aagagatgaa atcaagcctg
     9661 taaccgatat tgataaaaca gccagattca ttgctctcaa caaaacgtgc tacaacggac
     9721 tttatagagt aaatagaaat ggcattttta acgttcctat gggaaggtac aaaaatcctc
     9781 taatttgtga cagtaacaat ctaggaaata ttggtaatgc actcagatat tctagagcag
     9841 caataaacgt tagcgactat aaggacgcat tacttaaagc agaagaggac gattttatat
     9901 atttagaccc tccatatcat cctacaagtc cgacagccaa ctttacgggt tattcagata
     9961 atggatttgg caacaatgat cagttacaac ttgccaaact ctttgttgaa ttaaatgata
    10021 gaaagtgcaa agtactctta agcaattcag atacaccatt cattagaaaa gtatattctg
    10081 acttttctgg cttta
//
LOCUS       DAOR01000249            2856 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_75502, whole genome shotgun sequence.
ACCESSION   DAOR01000249 DAOR01000000
VERSION     DAOR01000249.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_75502"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          896 a          531 c          477 g          952 t
ORIGIN      
        1 gatgcattcc aaaaactatc aaatatgtat tgttgatgct ctgcaatttc cttaacatcg
       61 ctataaatca tcatttctga agactttccc ttctcatgaa gaatagaagg tgcagcatat
      121 ccttgttcac ttacgtagaa gtttccttta ataccattca agtggcggag ttcgtcaact
      181 actgagatca tctctttgca ataatgaaga ttatctttcg taatttcggt aatgtatctt
      241 attgttacgt gccttctcct tgcatcaata aatgcgtccc tgagtcgtat aatttctata
      301 gctaaatatg gccgtgtatg gtctacgcaa acatcgattc taagtttcgc tttgcacata
      361 aattgtgcta gcccctcaac aacattatca cttccatgta ctatctcagt cttggagatt
      421 ccgtttgctt ccgtgattca tatacgtgag tcacttggat ataaatagaa tatttcaaaa
      481 tctccgtgta gaaatcgcaa ctctaaatta tgatgtagag cttcagaaac gaactttgac
      541 taaaatttgg ggttgtggat tcgtttatca acgcatttat agcagcagaa caactaccta
      601 ttacgaggca cttctcatca agaatttatc taggttgtag actcttactc aattcatttt
      661 catcacctaa tttaggtact tttggccttc agacgtcaat tttatagttt gcgttggata
      721 tacattataa gatagcattt actacatcat cagcatcacg tattatgtct gttctttcag
      781 agatagacgt atctggtgat gaagaggcca cttcactcaa agatcgattc agaatgacgt
      841 tagtctacaa tatcaacgac ctataagggg atagaacata gaactaatct aaccaacctt
      901 attgttccta atattcttac caatcaaaga tgacaagtca gaaaatctaa atggtttcgt
      961 taatatccct ttattgttct tgattccaat tgagtcaatg tcttgtctaa taatatcaga
     1021 cggtaaagtg gatgtgaata taatttgctg gtctggcctt gtattggcaa tctccttagc
     1081 aacctggatg caaggaatat cctttagatg cgtatctaat ataattatgt caaatccgtc
     1141 tgtcttgtcc ttgtctttgt ctacatctgc gcttttcaga atctcatcta aacatttctt
     1201 gccgccattt gtaatcacta tctctttgaa acccatagaa cgtagccaaa tactatacat
     1261 ctgttgaaga tccgggtcag gttctgcaac aagaaccctt attggtaatt cttctattga
     1321 agaagaaaca cgcgatgatg actgtgcaac ttgaaccttt ttttctttat aggcagccgt
     1381 tgcatattgt gtcaacaaat aaccggccgt atcaataatt atgctgtggt tatgagaaag
     1441 ctgatgcttg tgctggtcaa atgggtgctt gcttaataat gagcctgaat gtgggcatat
     1501 aacattaata atatgatttt gattttgtgc tcttgttttt tcttgttgct gtagccattt
     1561 tatataaacg tcttgccacc atttctccac tatattgcat tgatcaaagt ttttatccct
     1621 aaagaggtta tcagcacaat cagcaacaat aagtactccg ttcttaccat ctctatgttt
     1681 tagttcttgt tgtaattgaa tgtaaaactc attaaaaggt gttaaatctc ctgctaatgc
     1741 agaatcataa aatggtttca gatttaaaat tatcaaattt cttttagtga tattttcttc
     1801 gtaatcttta atttgccagg atatctttga taagtgtgat gtattataag catcaacgga
     1861 agcataaata caaagctgtt tttcctgtaa tccttgattg atgtatttta tagatgcaag
     1921 ctcgcggtca gaatagtcat tatacaaaag cataacatga tggctgcaag aagatagatt
     1981 aattacatta gacattacaa gattctctct ccgcaactat cattttgaac gattcttcca
     2041 ctccttctga ttcataatgt agaagcatcc taattatact ttcttacttc aaatccgatt
     2101 cgtgtggttt accaataatt aaatatatca cacccttttt tgatacgtat gttcctcaac
     2161 tcgtttgaga gattcttacg caagttttaa acccaaaaaa tgtccataag ttggaaaaag
     2221 aaattaaaga tattttagcc aaaactaatt ggatgcttag aggaaaacgt ctccaaggtt
     2281 gtgtcaaact cgtacagaac aaaacaaaat cagagtatct tagaaaatgg tttaatgtca
     2341 aagctcttgc cgaacttgtt cctttgtgtc cgttaatgta acagtttatt gtaaaaagta
     2401 ttgtttcagg tctagaagtg gacaacatcc gtggcggcct aaggagcaga ataatgtgca
     2461 atcgaaaatc tgtaatggac tcctccctca gtacgcaaaa aaatgttgca agaacaaaga
     2521 taaagcggag ggtgatcgaa ttgccttatc tatctgcagt aaagcataca ctcactagtg
     2581 tgtgtacatt aatgacaaac actcactaac cacttgaata aaaaaccgcg acatgcagaa
     2641 gatactattc atagtgactc cagatttcta agtcgaaatt gctaactgtt ttaaatcaga
     2701 atggttcaac cgtaataaaa aaatggtttc tgtgcatttt tgctagaaca ttaattgcta
     2761 acctataaat ttcctgtagt aaaaagagct agtaaaaaca caaaataatg actagtacaa
     2821 acgaatcaat gcaactacca atagatgaat gtgagg
//
LOCUS       DAOR01000250           11951 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29761, whole genome shotgun sequence.
ACCESSION   DAOR01000250 DAOR01000000
VERSION     DAOR01000250.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11951)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29761"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3690 a         2375 c         2300 g         3586 t
ORIGIN      
        1 gggatgatgg cctatgatct aaaaaaactt gaaaattcaa aacggatcaa agtcagcaga
       61 tctcatagga ggagtacaag gttttaccct ttgagcatag ccgctcgaga attacgtgtc
      121 atcgagttca taagacgcag gactgccaga caaatcgtgc ggttcttact tcaagatgat
      181 tctcgtactt tcaaagatat agtacaattt accaaaaaag tacgttcaac cgtatcatgg
      241 catctttccc ggctcagaga aggagggata atatcggttg gggtaggtag gaatcaaacg
      301 tatagactca aaaacagaga tttagtaatc aaagtaatga agaagattaa tagttaatga
      361 cttggtctaa cgccgttttt tctcactagt tgttcgctat gcttcaaatc catcacggtc
      421 ttggcataga atagggcact tagtataata cccgctgcaa cagaagggcc tactatctcg
      481 gcatagctga atcccagtga aggaatgctt gcttgattgt tattaatttt gttagtagaa
      541 ctaatacctg gtaggcttgc tatagagtta gacagcgatg gctttgcagt gctagttatt
      601 acagcgagtg aattccctct ctgttctgcc aaccaaacat taccgtgaga atcagatgtt
      661 atccactgaa caaacggtga ctgttgagga atattgactt ctttattttc tcctgtccta
      721 gggtctatca ccgctatttt gtttattgtg tgttctgcca cccataaatt acgattattg
      781 tctagcgtca ttccaaatgg aagcccgttt ggatcgaggg gcgagtattg attaaaagtc
      841 ttaagtacgg tgttaaaaat agaaaccgcg tggccatcat gttcagagat atagatatct
      901 ccagttgtcg gatcagcgag cagggcagta gggcttttaa gtgtattatt tgtacctgca
      961 ataggagcat actcgctcac tttgtagttt ttcgtgggat cgatgttggc taattttcct
     1021 attcctgatt ccgctatcca tatttgacca gactggtcta tgattatccc taacggagcg
     1081 gcattcatgc ttggaagttt tattggtgtt gagaagctct tcgattgaat attgaatctc
     1141 aagatctcat tagcactggc cagagaaatc caaacattag tcgagttcga gtccacggct
     1201 atccccgatg gtactccttg aactggtagc ttgtacaact gtgctgtgtt agtttctggc
     1261 ttgtaaagac caagatcttt gattatagga tcgataaacc agatatcatt acttgaggga
     1321 tctagtgcca tggaagtaat tagactcgta ccagcaattt tatgctcagt atatttggtc
     1381 gtattcagat taaagtcaaa aatcctacca ctgtttatgc tactatcccc tgtccaaatt
     1441 aggtttctgc ttttgtcata aagaatgaat aatggctgag cattatttgc aggcatcttg
     1501 tattctttaa cgctgaagac gaaatctcct aatttgggtt taagaaacag attgtattct
     1561 gtaacaatat caagcgcgtt cgcctttgcc tgcattgctt caatttgtag attccatggt
     1621 ccggcaagtc caaatgcagc attaactgaa aatatccctt ttgaggcctt atcagcgtct
     1681 acagttattg gtccaatccc cttctcagtt tgggtgtatc ttagctgaac agacgatata
     1741 tcaattggat ttccgttgga atcagtatat gaaattttaa aattgttatt tcctggcgta
     1801 tagggattaa ttgatagggt tactctatcg ttattttcaa caaaattcgt cgttgtaaaa
     1861 ccttgctgct gtgttgtaaa tgcatttgct atattcaagc ctgggaggtt ctgttgttgt
     1921 tgttgtagct gttgtagttg ggtctgaaat tcacctgcag gcaatccagt attaacgagt
     1981 actgcaacag cggccagcaa tgccaaaccg acaaatgctt ctgcttttgt agtcttggca
     2041 aattttgaaa taactgcgtt gccattaaga taatggttac tagacccact ttcagattcg
     2101 gatccttttt tggcaactga tttatctatt ttggattgac caccccctcc ggcaatgcta
     2161 gcagtcggtg ctacaagtac cactttcaga ttatctcttt gaataatcat ttgattataa
     2221 gctccaatcc ctatcatcac ggctgcgaga atgagcttgg caatcaatgc ctttccatac
     2281 aacgacgcta gtgttaaagc aagattgggt tcgagaaggt agagtaagaa cggtccggta
     2341 attacgataa tcccaagaat tactacagga attgttgaaa accgtgggat aataagcgac
     2401 agaagggatg ccttaacata tccgtctgtt tgggatcgct taacagcagg aactactaca
     2461 aacgcaagat atattacacc accaatccac aacgaagcag ccaagttgtg tgtaaaatct
     2521 atcaagatcg gtattgatgc aggatttagg gatgctccat gaccaattac actagtcgtt
     2581 actaacgcaa ttagtcctac tccgagcaat ccatagataa tcctattttg ggtgattatg
     2641 cctttatttt ttctttggtt acgaaacaaa acagcagaca agccaattaa aacgaacgat
     2701 gtaactaatc tgataaccca aactccacca aatttcgttg caatagcatc aactatcccg
     2761 acatctagtg acttggcctc agcgtagatc attccaaagt cagaggctat aagtatgcca
     2821 gagccgataa tcatcagaat tgcaattctc ttgtcaatca ttcgacgtat ttcctttatt
     2881 gcacctgaaa accaatcgat tttagttact ggcttccaca accacaatgt tgcgaaagct
     2941 ccaccaacta caataacttg gccgacaagt gttgggaatc tagcaaatgc ttctgggaga
     3001 tagagctgag aagattgacc attaccaccg tttgcaatag cggtagggat tgcggcctgt
     3061 ccgaccccaa atacgaacgc attttctgta acatgcccat ccgtttcgga aagcattttt
     3121 gtagaaaccg tatatattcc atctttaata cctggcggga gtgtaacgct taaagcagta
     3181 tgatcgccgt tgacatagtg gtcatctttc atatcaattt ccttcccgcc ggcatccagg
     3241 acttttattg aactataatg aatgtcgacc ggttcgctta ggtggacgac tactttagat
     3301 ggtggcgtag gtaatgactg agacggggac ggatcgcttc ctattacaaa tgcatgagcg
     3361 taagctttag gtatcaaagg aagcattggt gaagccaaag atatcgaagc cgcgatagcc
     3421 acaatcgcga aataataatc aaacttcctc ctcttctgcg taatcaaggg ttgtccttaa
     3481 tttttatatg gcatacataa tataagattt tttctgtttt actagtgcaa gtatcgaatc
     3541 aagtgttgtt gtccgcctat ttatctgtat ataatagttg atggcagtta gatattcgac
     3601 gattacacca taaacattaa tagataatat cctacataga cccttgagga aatatcagtt
     3661 cgtcatgctc cagcttagta tccgcgcatt tcctgcttta attatctgct ttttgattgt
     3721 gttggtggta ctatctcaaa atgttcttat caagctagct tcggcccaca taactaaaaa
     3781 gtttggtagt atccaagtac aagttggttg gaacggtgaa ccgccgttaa caggtcaact
     3841 taacagcgta atatttgacg ttaatcagac gagtggcaaa actcagacac ctataattaa
     3901 tgccttggcc aatatgaaca tacttgtaaa gtatggtgga ataacgaagt cgcttgattt
     3961 tttaccctct ccaacaaccg acggattata tcttggcaag atgatcccaa gtagagttgg
     4021 atcctattac gttgttctca atgggacgat acaagggcag aagattagtt cccaattacc
     4081 tctcgatcta gtagacagta gtcagaaact ggccttcccg gatacagtaa ctggttctgg
     4141 cacagatgct acagtcacag gtggcagcgc tagcactagc actaacacaa acaatatagg
     4201 accacagcta cagggtattg ttagccaact tgctaatcag attgattcgg taaaaagtgt
     4261 gataggttcc gttgccaaga gcaatgcgga taccatgaaa tcggttcaag atgtaaagag
     4321 tgcagcagat cgatcctact tgattggaat ggttggtata ggggcaggga tagctggaat
     4381 agttattgca tcattggcac tttctcgaaa acgtgcgatg taagcttgat gctgccgtcc
     4441 ccctcgtcaa taacaatcaa ccctaaattt ctgtacgcaa tcgacttatt atcgaatctt
     4501 tttgctgtct ctagtgaata accttggaat aacgaccttt tacttggatt tctacattta
     4561 gctcctttgc gtttgtcttt ttgggcataa ctgtatatca agtccatctt tcgtggctga
     4621 atctagcacg gcatcattgc cgtcgcgtaa gtgactgcct aaaaaataat gctattgttt
     4681 atcatcattg aaaataatgc ctcttaaatc atataataaa tttcattctc ataaattaac
     4741 ctcataaaaa tccatttttt taagcttcca atactagtat agacagacaa gcctactcaa
     4801 aatgaatata taaccagtat caatagaact attctaatga atattagaag aacatttgga
     4861 acatttccct ttgtagtagc aatatccctt cttgcaatag caggtagtgg ttttgctgtt
     4921 atatcctcta atacggcttt cgcacaacaa caacatttca ctgccaaact atcaggggcc
     4981 aacgaagtac ctccaataac gtcagcggcg tccggcatgg cagcgtttga tcttaacgca
     5041 gcaggtacac aaatgaaata taccttgaat gtaaccaata tcaaccatgt aatagcagct
     5101 cacatccata tgggctcaag tactgccaat ggaccaattg tagttaatct gtttattcct
     5161 tcgaaagcta ctggtaaagt agatgggaca cttgcgcaga gcagcattaa ctctactagt
     5221 ctgataggac cattaaaagg taaacagatg actgatttaa ttagtcttat caacggtggc
     5281 aaagcgtatg taaacgttca cacaacacaa aacccgcccg gagaagttcg aggacagatg
     5341 tcatctggaa gtgctacttc ggcatcaaca gcaacagcgg catcaacagc accagcacca
     5401 tcaacatctt caactccaaa atactaagac aagaacaaaa actacattgt cataaaagat
     5461 aggcaaaagt aagctctcat ttctttttat ttattgttgt agttatagat tgtatcttaa
     5521 ttgttaaatc tcataaagcg gtattatcta gattatcgga tcatttactc aacagattga
     5581 aaattcgcta gccatgcaca caagtagcaa ccacaagaat acaattaaat gatcatagaa
     5641 taaatcccat cccaatgacc attgcatatg tacgttaagg cgatcacaaa agttgcaaat
     5701 agacatcacg aagcaacgta cctttattta gtgtcggtca tatggctaga acattccaac
     5761 ttatacaagg tcatataagc ggaccactac tctgatgtaa attatcacta catgaatgtt
     5821 ctgtcaaaac actagtaaat acgtgaggga ccacgacatc gtagaatttg tgaatgtccg
     5881 cacaaagata accataaagg gaaaagcaac atattcacaa attctttcca tgattcctgc
     5941 agaaattact acacgaataa attatgatag gtcacatttg gaatatgtga tttaaactgc
     6001 gtctgtcttt cgtgccgatg atagctacaa gcaagatttg gttcgtccaa gtattgacac
     6061 cagggcccac tattattatt attattataa acgagtcatt cctgtttgtc aaccctgaat
     6121 attcctatac caaacaatgt cagcattact aaaaagataa catgcgataa ttctatatct
     6181 acaattggaa tcagtatgtc ttcaatcttc gacgtaacat caaagagata catggcctcg
     6241 atgattgata gcaaaaggaa gcggacgcgg tagtgaataa ctatacggcg atggtcgagg
     6301 agctttagtc aacagctacc tagttcgagt gagattatat cagagccagt ctaacccgcg
     6361 tgttgtgtaa agaatgatat attagcgaca cagcattgat tttaaaagta tcacaccttc
     6421 aaagtgtctc agcgctattc tttttacttc ttaaggtcat catactacat gtttttttct
     6481 ttgatgatga aaccactcat cttgcctatg gtcatttctt tggatctatt aagcaaatcg
     6541 gtaattatca aatagttttc cagcctcttc tcatttgcga atgaagaatc aaccctaaat
     6601 tttagtatcc tcgataaaaa taatgcaaat gcaaacaatg tctatgctgc attgactata
     6661 aaagacaaag cgactggtca attggttgag caaaccccgt tcggattcta tgaatttagc
     6721 gatatctctt tccctcacaa agaagcaact caaatttcgt agtaactttt gatgccacaa
     6781 taaacggcga tccaatatat caagaaaatc cgatgattgc cagttttgat ctgtttgttg
     6841 gtaatcctcc atcaggtatt ccttttggca gagcagtatt gctttatggc cttcccaccg
     6901 ctacaatagt gccgattgga cttgccatat accatcggta tgggaaaaag agtacaaagt
     6961 agttctttca aacacaaaga tgtttttgca attcggtact tgtaccacaa actattttat
     7021 gtattctaac atttcatatt caatggaaaa gcgactaaaa agtaattcta cagcaaagag
     7081 tactgcccct attgttattt tgctactgct aggttcattg tctgcagcaa taaccactcc
     7141 tttcaaacaa gtagcctacg cgcatacttt tactggagat gagagcgcgt catttctttc
     7201 agttataaga atactgcaag ctgaaggtaa tcttgtacga acaaatctag ctacaaatct
     7261 gcggtaaaag tgaatttctg aaaatgatta gagcagcata cgatgacaaa tcagtattca
     7321 ttgatgggag ttatggatca aaagcaggaa tattcgaagc gctcaaagag cgcaggccaa
     7381 aatatgtatt gctagatgaa gtcgataaac ttactggtca agaccaacaa gcgctgctta
     7441 atttaatgga aagtggaaga ctgacgaaaa caaccaagtc agaaagttac gacataaaat
     7501 tagatacttg ggtatttgct acagcaaacg ataaggagga attactagaa ccattatttg
     7561 accgttttga agtataggcc tataccgatg atgagttcaa agcaattgct gcaaagcgat
     7621 tgagcaaaga aggaatacag gatgtggaac tagcattata tattgtaaat gcagtcctaa
     7681 aaggattgca caaaaggtca tttagacaag caattagaat tgccaagatg tgcaaaacaa
     7741 ttgaacaggt ggacgaaact attcaggcac ttaagaagta cgatctaaaa tgaacatcag
     7801 gaagtacata gacaaataat tgaaatcgac gtaggtgaat tttcattcca aaagaccatc
     7861 gtctacatct ttcgtgcttg ttgcctatat taaacattag aagtatttcc tacatttctg
     7921 cggcttgcat taatcaatca aattatgttt gtaggttaga aaatataata ggacttggat
     7981 aatgatcgaa tcgttaaatt gcaactggga tatcttccac ggacaagcac atacctagta
     8041 tgaattgaga atcgttatgg ttggttgcaa aaagcatgat ggatgatatt ctcgacattg
     8101 accctaactg ggatatttcc tttgtgacac aatatctaga cttcattgga accgaataat
     8161 aagcataaaa taacgtacaa ctcatttttc aacaaggcaa gagcagccct ttctagcgat
     8221 aatatcctcg ttcatttctt tgggccgtcc tgaaatatta taaaagggcc ttgctttgtt
     8281 aaacgtccca agtattttag atcgccaaat atcctaggta ctacttcctt gattctacca
     8341 ttccatgact cttgaggaat atcgacggga acggttgtag ccatagtatc gatagtatag
     8401 ccatagtaat agactggcat ttcttaataa cgtcgccggc gatcttcaac cggaaatcga
     8461 tgccgagggg aatttttccg gataatattg gaatagagat aagtacccta tccctctttt
     8521 ctttatctga ctaatttata tcccacatcg caacaggcga tgtttttcaa ctaaaaaaca
     8581 ttccggattt gggcaaacta tcgctgttca gcccttgagt gttagcacca taggcatcgt
     8641 acgtaccaag agtaccgtga gcaataatgg gaaaaatgat tgaattggaa gagcgtacac
     8701 acgtcgtcgg tatcactagg agtagacccc gtctcagtta agtgctccaa tggatatatc
     8761 gacggtcgaa ccattcatcg atccatatga gcgaattatt taaattggaa cagaaaatta
     8821 aagacagaat cgaacaaata agacacaaaa tgcacaagac tcacaacaga gcatacacag
     8881 atagtctctg gacagagatc gagacattga attgggtttt gaatgaaatt ctggtcttga
     8941 gtcgaaccgt atagactagg cggattgaag gaattgcgat attccgtagt aggaattgca
     9001 ttgtctagtc aaggcaaaaa ttttagatcg gggatctgag atactagcag aagccaagtg
     9061 taagaatgca ctacgagcgt gctaaatatc atatatgctt tattgatatt ctctcataga
     9121 cataggcact ataaaaagga gagaattgct tatcctggaa atagataaag attgctaaaa
     9181 tgggtgctgt atacttgccg tcggattagc cttctaaaat aattgtatgc ggtcgtcaga
     9241 taaagaatga aatagtcggc cataccatac tttcagtgtg ctctgattca gggtttaaat
     9301 accctgcaat atcgcactgt ccttacaatg acaggggatt ggttcatacc gctggtgact
     9361 gcggcatctc aaatgacgtt caattatata caaggcttga taaatcaaag caagataaac
     9421 aacaaggatt ttttacacat tacaaatgtc actctagaca gctcgtaaac gcgctaaaga
     9481 gctggaaaca aggaccaaat acttttacta catgcgaata tagagaggta cattagaaat
     9541 aaaatctgca gtagagtata ctaaagtacg gtttatgaag tatatgatga aacatttaga
     9601 ggacgcatat ccaaaggtat tagaatttat cactgagata gggacgaatc atatagaaac
     9661 gtgtaaaaaa attcaggaga ttatggtatc tgatacacca ataaccgtga caccatcatt
     9721 tcaaaagata attacagata aaattgcgtc tgcctgtcca ggcttaaaga aaagtagaga
     9781 cacttctctc acagacaata atatttatct tcatattttt gttttcaggg taatatttga
     9841 taccgtgtat gcaaaagagt ctaccatcat cctttctgaa aaggtgataa gtaataacaa
     9901 aagccaatta tggcatggaa accagcttct tgccttggct caaggagaag ataggctaat
     9961 caagaaatta aaagaagtga taggcgcatt agtactaaat aacgatatca aaaatagagt
    10021 cgaacaatat tctgaattac gtaaacaact aatcacgaat gaaagaatag atgaattaag
    10081 cgatgatatt gaatatctgc atgaatatat ccatggcgga caagtattgg ctggctacgg
    10141 tgcatgtgat ctatgtcctc caccaaatat tagcccttga atttactcgc attctcaata
    10201 acagtcttcc acttgttttc gccttcaata ataattccac atttctcagc aggggttttg
    10261 cctttcaacg cttcatgtgg tctgaaataa ttatgataaa tttggtagcc agacagaatc
    10321 ttcgtatcag atttctttaa tcctctcatg gtcttttctc tgtcacgcac ttcgccattc
    10381 aaacgctcca ttttgttatt gttgtggtct acaaagaagt atttgagaga cgcaattagt
    10441 acgacgattt ccattaaggc atcacgatag gaatacttcc aagaaaccgc aagtagagca
    10501 tatatagaca gtataatgat ggtatttcct catgataaac ggtagatagc tttcgactgg
    10561 tatagccatt gcatgtatga ttggtaatgg gatttgtttg gggatttctt gggtctctcc
    10621 ctccataata ccattcggac actttgaaca tggcctaatc ataccgcttt tcctttcttt
    10681 gctgcgttct caatcaccgt cttccattta tcttggcctt cgattattat tccgcatttc
    10741 tcagcaggcg tctttccatt caatgccata tgtgccctaa aataattatg atacaattga
    10801 taacacggga gaaccgctgt gctttggatt tttaatccgc gcatgttctt ttctctatcg
    10861 cgcacttcac cgttcatgcg ctccattttg tggtcacctt gcagtcgaat gtgagctata
    10921 tgccttgtcc tagaatcttt tagcgtaaag aattcttttt tgaatgcgtc tgcgaaattt
    10981 ggcgcgccat ctgttatcat cgtgtttggt ctttttcctg caatttcttt agcatcctta
    11041 aacaatggtt gaatatttga agtatacttc gtatcagcga cttgctgtgc tatccagaat
    11101 cttgtttggt cgtccatcaa tgcgaataaa tatttaggat ttcctttaat tttcaaatac
    11161 aattcatcgg cgcgccaagc atctgaaaca tttggtgtta ttttttccaa atacgacttc
    11221 attaaagtaa catacttttt tatccaatta taaatagcga cgtggctcat cttcacgcct
    11281 tgcaatttca agaatttctg cacatttctg aacgattcgc ctgtgaaata caattgtaat
    11341 gcagatgtaa acatttgggg tgttgcatgc atcttttcaa atcctagatt aatcgtaaag
    11401 tatcggttac attctctgca attgaatttc tgaatcgcgc cgtatttgtt atgtcttagc
    11461 ccatctctta cgatccacga agatttgcaa taaatacagc attgtgtatc aatgggctca
    11521 atgcgtgcga cttcgacttc ctttcttaat gcaaacgata attcaacaac aaatacatgc
    11581 ttacatttaa cacctcgaaa tttatgggca ggacatgaac aaacccagcc taagtcagtc
    11641 gagcaaaggt catattctcc attagcattt tgagatttta ctttgtatag agagtcatta
    11701 attcttttta tcgtgccgtt aatgttagca atgactaatc ctccttgctg ccgattaata
    11761 gctgttgctc atatctacat agagaagaga caaatcccag ctacaccgat ccgacccgga
    11821 gaagtgagca taaaaattta gaaaggacgg agcctagtga tgtctatctc ccgctgctga
    11881 cttcttaatt tctatggtaa tcttatcgcc cacttttatg gccatatcct cgtactccct
    11941 catactgatt t
//
LOCUS       DAOR01000251           25253 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_25903, whole genome shotgun sequence.
ACCESSION   DAOR01000251 DAOR01000000
VERSION     DAOR01000251.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 25253)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25253)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25253
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_25903"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    20362..20519
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22221..22272
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22931..22986
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7566 a         4910 c         5300 g         7211 t
ORIGIN      
        1 acagacatga attctatgtt ctatagcagt ggcttgcaga tcgataatgg tatattccat
       61 gaatggtgtc ttgatttctt cagatataat tggtataatt cgatgtcttt tgacgagagt
      121 aagctcatag aagtctgaag aatattattg ggctctggct tgcctcaaac gcacctttcc
      181 cggaattgat aagcaaagcc acattgtata ccaacactga acgaaattgt tgccttgcaa
      241 aattaatgga atgtttggaa ttcgtacagt tctgtcaatg tttgccttaa attgctaact
      301 caaatttaca taacgacctt tgataagttt atagaagcta aggatttcaa ctcctaaaaa
      361 tatattaatg gatggcctct aatacaaagt ggcttatcat cggtctaaat gttcactatt
      421 tcctgcctat ttgatatgca atcttgttgc taaaggtgag gcaagaaata ccgaaaaagc
      481 tggataaata agagacgtgg gagaaagtca aaaactaaac aaagctcgta tagaggagca
      541 agaaaagctt gtcgataagc agagcggtga tgctccaaca agagataagg gtactaacac
      601 taacggctct gacaacgaca gtaacaatag gaagcataag catcaagtac agcccattat
      661 tccaaagaag gacaaagaaa atgagaatcc attcgctgca aagttgacta cagacagtga
      721 aggcgaggtt gcaaaaacta ttttacaatc aattccaaca gaatcccaag ttacatcagc
      781 taagttcgaa ggtcccaata tcgcactata tacaaaaaat gctaaattct cacttacgga
      841 actgacttac tacctatcgt ctctgtctaa aagtctgaaa aagagatttg ttattcgtac
      901 tgacccttct atcagaatag cagaagacga aacccggaaa gccgttgtca aattactgcc
      961 caaagacgtc ttagtttctg ctgtcttctg cgatgatgct actggtgaag tcgttctaga
     1021 agttaacaag ccagaagttg taagcccgga attagtagtg gaaatagctg tatccacagg
     1081 ctgggtagct cacactagac tttccccaca tataccatct gccatactca atactatcca
     1141 ttctacccta aagagctcgg ctaaggaacg gattatattc ctacaggagt taggaaatag
     1201 gattttcagg accccacttg cagagggaaa caacggtagt ggaaataatc agctgaacgc
     1261 cgccgctgaa tcatatagtc gtggtgggca gactcagtca tggtcgatga acaaagaagt
     1321 catgctgttt tgcttgggag gcgttaagca ggttggaagg tcatgtttta tagtcgtaac
     1381 tccagaaagc aaggtaatgt tggactgtgg cattaatcca ggtgaaagcc gtggcttaga
     1441 tgcttaccca agacttgatt ggtttaattt tagcttaagt gatcttgatg cagtagttat
     1501 tagccacgcg catatagatc atcaggggtt cttgcctagt ttatacaaat atggatacag
     1561 tggtcctatt tactgtactg agcccacgtt acctttgatg actcttcttc aaatggattc
     1621 tataaaaatt tcgaagagta atgggacata cttgacgtac gaaggtaggg acgttaatga
     1681 ggcgatcaaa cactgtataa cactgccata tggaaaaccg actgatatat ctcccgatat
     1741 tacaatcaca ttgaacaatg caggccacat aatgggcagc gcaactgtac acctcaatat
     1801 ctctggtgcg cataacatcc tttactccgg cgactacaaa tacgcaaaga ctcagctgct
     1861 ggacagcgca ttgtctatat atccaagagt tgaaaccttg attacagaga gtacctatgg
     1921 cagcagtact gacataatgc ctgatcagtc agttgtttat gcaaccttta cacacagcat
     1981 taataaaaca ttgacggaag gtggtaaagt cctaattcca gttccagcag tcggtagggc
     2041 acaggaaatt atgttagtga tagataagga aatgcgcgaa ggtcggctga tagaatctcc
     2101 catatatatt gaaggtatga tttctgaagc gagtgctata catatgtcat atgctcatta
     2161 ccttggatat gacgtgcgca gatcggtttc tcaaggtatt aatccgttcg aatcagaata
     2221 tttcactcag gtaaatgggc taagtagaag agacgaactt ttaaatgatg agagccctgc
     2281 aattataatg gcgacctcag gaatgcttga aggtggtcct tcggtagaat acttcaaaga
     2341 aatagctcca tcaccaagaa ataagattgt gttcgtgtct taccaaatta atgggacgct
     2401 tggaagaaga gtcattgacg gcactatgac agaggtaagt atgttagaca agagtggcaa
     2461 ggttaaagtg gtgccagtaa gatgccaaac tcacaagatt gatggcttca gcggccatag
     2521 cgattttaat cagattctaa actttgtgtc gagaataaaa ccaaagcgag tacttgttaa
     2581 tcatggtgaa aagtctaagt ctgaaaacat tgccagtgct gtatatcata gactaaaaat
     2641 ccggtcaagt gttccggaca atagggaaat tatcaaatta agataaaccg atgtatttgt
     2701 atacacacct ttttagccat gtatcacgag ttataatgtt agattaacga attggcaata
     2761 agatatagat gcgttagctg caacatcgtg ctgagcggtg aaggagcctc cagacactac
     2821 cttgctaacc caactcataa actcgagaaa cttcccccac cactaaagcc tgtaaaaaat
     2881 acggacgtaa cgcgatagtt tggtttattc tcttatactc tctagggtcg agactacttg
     2941 ccaaagcata gtcctaatgt gtgattgcat ttggggattt tgggtaacag tgtctaacgt
     3001 gctaatggcg tttgcagctc ggacagacac agttccactc ttttcatctt ttaacgcaaa
     3061 gtatacctct tttatcatat tccggatgtt cctctgaata ttgatattct tggatatggt
     3121 atctaatgtg gctattgcat ttgacaaacg ctcctcgttt tctttccgtt ttaattcttt
     3181 ctttgttgtc gtcatatctc tatacaccct ttaaccgaag actgttttct agtttataac
     3241 tagtagcttt tgaatctttt tgtcgtttgc tagtcctatt ttacaactca agataatatt
     3301 attccaaata atcatcctgg tgaataggtg aaggacaccc attcactccg ggcagctatg
     3361 aagtgttgct ttagctgtta atcataatct aacaaactag attgaatcaa agttgttcac
     3421 aactagacag tcacatgctt cgaacatggg ataaaaaaag aaaatcataa gtagatcgat
     3481 aaagcatttg aatgtgttta aggggctttt tttagcaaaa ccaaaaaata cccaaattga
     3541 attcgcattt gtgatcataa tctggttttt gacgtattta cataaattta tttagtctaa
     3601 tatgtaaagt tgaccgtgaa cccacatatg caacatcggt ttactaacct cgtccaattt
     3661 tctattgtca tcccttctcc tgcagacttt gactaggctt tattttttta gaaattagat
     3721 tcttatagcg catagatggt ccagtattat tacgaacgca ccgagcgaac aaggggatct
     3781 gatccgttgt aacatttgtg agatgagaat aaatgtggtt gctattgcaa agcatacgca
     3841 tgctgcttat catgaattgc ggaaattgga tttggaaaaa gaggttaata gggtaaggac
     3901 gactgattgt tatgaaaaca ataggtcggt tgtttccatg tggtagacgc tgatccctgc
     3961 ttcactcaga caactttgtc aagtgaacgc tgaggtgatt tatatatgac ctataaaatc
     4021 agaaataaat tttacggcca cagctataag tgatatagaa agtagcttcc gtagtagact
     4081 gtcttttact ctatctgaga tttttgcacc gatctgtcct cccacaaacg cgcctgcgga
     4141 caaagatgca gcatgaaaat aatcaggatg tcctagaacc gcgtgggtaa ctgtgccaac
     4201 gaaggaggtt atcaagagtg caatctgtga agttggactc gcggtggtta acgtcatgcc
     4261 caagacaaga actaggagcg gaacaaagat tatccctcct cctactccaa agaggctaga
     4321 gattatccct gcgcccaatg ctccagaata gaacaacgga cggaggagga tcgacttcct
     4381 attattcccc gttggtccat tttgtctttc ttttgtgaag gaactattgt aagcaatata
     4441 tattcctgca aacatcagaa gtattccaaa gtatattttg aagggctcta ttgctataac
     4501 gcttgaaata aatgccccag ctatggcgcc cggaattgct acagccgcca ttttcagccc
     4561 aaagtgataa tgtatttttt tttgcctggc atagcttatt gtcgaagaag cgctggtaaa
     4621 cgttactgca aaaaggctcg tgctagaaat ctgagcagga gcaaatccca taaaggttag
     4681 agctggcgtc attattaatc cgccccctac tccgatcatg gatcccaagg tacctgcccc
     4741 cagaccaaca atgattaaaa gtgcagacac agcaaagggg tcaaacaaag gtggacacgt
     4801 atcctctctt ccctgtagtt ttaatacgaa taaatcattt tctatttgtt tggaatccga
     4861 accaaagcta tcatctttat ttagttcgtc atttgtgact aagtttaact agtattcgtc
     4921 ctattggaag ttaattatta ttagcagatt tttttcaaat ggttagcaca aaataggcac
     4981 atcctaaaat aagtaacaac tatctgaaat cagaggtgtt aatggtactt gcaacagccc
     5041 caatagcagt gtaatttatt cttgaaggat tcaattcttt cacccaatta agcagggaac
     5101 gagcactcct ttcagctcaa aatattccag aactaaaaag gaattgtttt attctaacaa
     5161 aaatcgtatg tttagtcggt aatcgttgta attcttagtt cttttctact gattttctcg
     5221 aagggaagta acgaattttt cgtatctgca caacttgccg ctaaaagata atcgtcgaat
     5281 ttaaatatat taaacccaat ttaacatcca ctttggatgc gtttagttgt aattggtttc
     5341 ggagtcgtcg gtcagagttt cgcaaaatta ttgctatctc gttccgccga tctgtatagg
     5401 ctatacggag ttaacccacg tattgtggcc tgcatcgaca gtggcggcta cgcggtttcc
     5461 gcaacgggac ttgatcttca gcggttactt aacgcaaaga aactgtcagg tacagtcggt
     5521 gcatacgata aagaaaatcg caagttcgat ccccttcgcc tcattgagaa ggtggatgcg
     5581 gaaatgctac tggaattaac ccctactaat ctcgataacg gtgaaccggg tatttctaac
     5641 atcatagcag cgatgaaatc gggaaaacat gtcatcacgg taaataaagg gcctcttgcc
     5701 ttggcatttc cttcactatt ggagcttgcg aattataata acattaagct tcgttttagt
     5761 gggacggtag ggggtgggac gccaatatta gaatttgcaa agcgatgtct taaaggagac
     5821 agcataatgt ctttccaagg aattctcaat ggaaccacaa actatatcct tagcaagatg
     5881 gaggagggac ttacctttga agacgccctc aacgacgcca aatacaaggg gtatgccgag
     5941 gcagtccctt tccttgacgt tgatggtttc gacgcagctg ccaaacttgt aatcatggcg
     6001 aattggataa tgggaatgaa agttactatt aaagatgtaa acagacgtgg tatcagcgga
     6061 gtcagcctat cagatgttcg gcgagctcat aaaaggggaa atgcgataaa actaattgca
     6121 acatgtgaca ataaacaact cgatgtgaag ccgaccgaac tatcaaatgt ggatccaatt
     6181 tgcgtgaacg gaaccctaaa tgcggtgacg ttttcgtctg agcaatcagg tgaacatact
     6241 ataattggtc aaggcgcagg gggcattcca actgcaagcg cggttcttcg agacctaata
     6301 gacattcgaa atactatttt tgaacaacga gagaatttat gaacagaaaa gagaaaaaaa
     6361 atgactgtca gcaccagtgc tactctaata agactaaatg aagctaactg aataatgatt
     6421 ttggactgaa ctaatcaact atattcttgc ttgcttatgt ctatcatgca gataacgatt
     6481 tccaatgata atcctcttca cccattcatg cagacaacag aaaatttttg ctttgtcaaa
     6541 aggattaata gcctgataag gtgtttctaa ataagtgtat agtggggaat tggaagtaca
     6601 ggccaagcgg aaagcattgg ctgttttgca agatgaattt agtaggattt taaattcggc
     6661 aagggaattg tctgctctca ctacctcact tgtcagggga gacattaagg atatacaggc
     6721 atcattggaa aggatgagaa ctgccgaaga agaagtagag aatctccgcc gcaaaattac
     6781 ccgagaagtt gcagaaatcg gaagcctgat ggtttacaga gaggacgtat tgagagccgc
     6841 ctatcttatg gacgatatcg caggttacat cagcggagta ttgtttagac ttgccaatat
     6901 gaaattaaca tctctaaaga aaggaaaatt tgatgaagat ttagagcagc taattggaat
     6961 ggtcgttgac gcgattttta aattgaacga aatggctagg gcactcagta ttaatccagg
     7021 aagtacgatt gagcttgcac aagatgttga aaaattggaa cgaaaagttg acgcaaggta
     7081 cagggccgtc atcataaagg cgctcagcga aatctccgtt actaaggatt tacttctctt
     7141 aaaagatgcg atcgaaggga tcgaaggtat ggctgataaa tgccaagaga cctctgattc
     7201 gcttactata ttggcactga gtatgtaatg catcctatca aagggatatt ggtagaaaat
     7261 agaataattg tctggaatat agaacactcg cgtgctttat ttggaaacgg atactacggt
     7321 aaaccgttag gagtcccaaa accaaaagga atccaatttg attccccact aattttggat
     7381 ttgatggagg gatgttactt gagtcaaata ggaaaactcg aaatatctag taatggtggc
     7441 agacgattat catccaaaga tatccgagct atatgcaaga aggaatatgc aaattttgag
     7501 ataaagtaca tggtttttca gaacctacgt gacaggggct atatcgtatc accaggtctt
     7561 aaatttggct gtgactttgc cgcttacgag cagggaccgg ggattgacca tgcaccatat
     7621 ttagtgcagg tcttcaagcc gaccgaccat atgacggcaa caggaattgt gctttttgga
     7681 aggttagcca caactgtgaa aaagcagttt atcctagcaa tcccgaggac taaggacaga
     7741 aaggttgatt ttgtcgggtt tgaatggtgg cgagcctagt aaaatagagc agggtgtagg
     7801 tttatcgatt cagggacaac tgagttgcag gaattttgtt cgaccaacgg gccttttgct
     7861 gtggcaggtc aaactttaca aagttttctc ccgagattgt gagacgagtg gcaaatttat
     7921 catagcctgg ttcgagccag cccttatatc tgatagagtt tttgacgatg tcaaatacgc
     7981 ttggcttttt ctcatcgact tcttcgtaac aggataaaag ttcaccatac ctgatttctg
     8041 ttttcttgtt aaaatgggat aaataatagg caaacacagc ggccttttca tagtgattgg
     8101 acggtttttt ttgtgcataa aactccctca atccaatctt gtcttcattt gcttttagat
     8161 gtacaggaat tggcgggaga gttagagact gttttgagga gctatgcctc gtattgttcg
     8221 tttgcattgc cgataacttt gtctctgtat ggagaggtag agatttttta taaatttctt
     8281 cttgtttgga aaggccttgt tcgactccca tgcccatggc ctctctaatt gcagaattga
     8341 agagttcttc aaacttttct aaatgcttgt caacaaatgt aacagtacct tctaattcta
     8401 cgataccttc cttcgcatta attttaaatt ttgcctctgg aacgctcaat gttggtacga
     8461 gtagctacct tttaattaag ttgtcggtac aataccgtaa ccttgacctc atcgtacacg
     8521 agagaaacga atcgatcatt ttagtgctca tcagccagat caggaccaga tactcactca
     8581 gaggttaact ttaatagtcg tgggaaagtg cttaatccgt gaaagcgcaa tcttttagac
     8641 cttctttgaa atttaacggt aaaatagttg taatcactgg aagcggtaca ggaataggtc
     8701 aggcgattgc taaaaaattt gcagaaaacg gcgcacacat cgtgattatg gggcgaagaa
     8761 gagaaccatt ggagcttacc tctgcaatat tatcagatac tgtaaaggct gccggttcga
     8821 ctggcaaagt acgatctttc catggggtag acgtggcaga cgaggttggg attaacaata
     8881 tgttcaaagt tattaaagaa gaatttggaa cggtcgatat tatcataaat aatgcgggag
     8941 tgtcaggtcc ggtgaaggta tttactagtg ccactcatgc ggaattcaag gagtgtgtag
     9001 cgattcatct tacgggggct ttttggactt cgctacaggg aattaagtca atggaacgag
     9061 gtggcaagat aattacgatt gccacatttt tcactgaaga gaatcgctat gagcaaagac
     9121 cgtatcgatt tagagctccc tacacttctg cacaaggatc gaagaatagg ttggcagagg
     9181 ctctggcttg ggagctcgta gaaaaaggca taaagtcgat tgctacaaat ccgggtcctg
     9241 tccactcaga tcgaatttat aaaactgtct accccaaggc agcagccgag ttcctaagaa
     9301 ttggtggcta tcaaggcctc acatctatag aaattgatag agttttaacc atattacttc
     9361 agctactcgg tcaacctgaa ccaattattt cggagggcat aagagaagcc gccgacgaaa
     9421 tctcgaaatc ccagaagagt aatagtactg actcattttc aagtgttgcg aaaatggagg
     9481 cagtactctc tgggttactt gcgaaggtac aagaaatcgc cgaaaagata caattcaata
     9541 ctgccaagat gatagtcgac ggtgaatttc tgactcaaga cgacgttgcg actatggtat
     9601 tgaatctgtg tgacgatgtt ataagcaagt taattaacgg tagggttgtt cccaacgatc
     9661 gcgtatttta tccagtcaaa cctctagttg gaacttcgat cttgggaaca aatttacctc
     9721 atccaaaaat taaggacaaa gttgttgtca ttacaataac atcttcatcg gaaaaggatc
     9781 tggatcgtgc aaataaaatc gcggcagcgt tgtcttctag caatgtcaaa caagttgtaa
     9841 tacttaccaa gaatcccgca gatttgacac gattcagagg tttccacagt cacttagtca
     9901 atctttcaga agaaaagagc gtacgaatga ttttcgaaac ggttagatcg aaatttggca
     9961 gtctagatgc gttgatacat ctcacaggag actacgatta tgatttacct ttttcctctc
    10021 tggcaagagc acaatgggat tcattggttg acaatttcat taatatcccc ggtttgatcg
    10081 tgaaggaagc ggttaatatg atggcacctg aaggagcacc ggatgaaccg gtgaaattca
    10141 gaggctctaa aggagtggtt gtaatcatcg gccctgatgc ccctgtgggt aaaaagattt
    10201 cgggaacagt cagagctcgg tcagaagttt tcagaggtgc attacggcca tatactgcca
    10261 ccgtgaatca agagcttgct gatgtcttgg gttccgatat tcgactctat ctcgtactgc
    10321 caggaaatat agaaggagag ccgcctgata ctgaaatgtt gaaaaactcc ttaactcatc
    10381 tactctcggt ttcatctctc aaaagaagtg aagtaatatt ttacatagat gaatcgaaaa
    10441 gttaaattat cattatgatg ataccccggc agagtcagca tactgctgcc cttggagaac
    10501 aattgcctgt ctactctaag aactaattag ccagcacaat aaaatattcc tgataagccc
    10561 taggattatg ctttgcctgc ataacctttt gatggttggc tgaacattag tattatattt
    10621 atattttgaa gttgtttctc atgagtatgt tcgatcctgc caaacttgtg gttgaaggat
    10681 ggaatggtta cgtatgagaa aacaggaaaa gtgaaaattt gctggccaca tgataaccga
    10741 agtgctccgt tccatcataa attcactaat aattcttgaa aagcttggaa cgtaaaagaa
    10801 gtatgtgatt gaaaaagtga ccggggaata aaaatacgaa agctactcag actgcgtgtg
    10861 aggcagtaaa gcagtttaac ttgtgacttg ctcgctagtt gattctgggt tcgacgttga
    10921 ggctggagac gatgatgtgg acgatggtcc cccattcaca atttcagcag tggcgaacct
    10981 gtttccgacc tgcaccactc gaatcttggc gttctgacct accttaccgt tcttcacaaa
    11041 gattacaaat ccctgtatcc ttgcaatacc gtcacctttc ctactgatct ccgtaatctg
    11101 tacgtcatag tctttaccag tttcgactgg ttttggacca aagttatcgt ttcttctgaa
    11161 tcctccgtaa ctcattctta ataccttaac tcaagtcaga taatgaacca tatataaact
    11221 gtcaaaagcg cgatccggtg tgtgtttagg ttccaactct aattccgggc tcgcatattg
    11281 accgcaagca ttctattgct gaaagtattg cgagaaaact ggtctttgga ttattcgggc
    11341 tcggaacgtt tcgcacagtt atagacatct ctccgaaagc acccgtcgcc tcaatttgat
    11401 gttgatttac agtaatatta ggatccgcaa taattctgac tttcgtttgt tgaatgccaa
    11461 ttcccgcaag gctgagtact gccgcaacat tgatattggc aggaaatttg ttgactgcgt
    11521 ctgctgcggt cccttgaaaa accaacgtct ttttttttat ggaattcaat cttgtaggct
    11581 cggaattaaa aaaaggagca ccggctagag ctctgggatt ttttgttgtt gtgagcgtca
    11641 ctgaatctag cattcctttt acactcttaa tcgcatctat gcccgcgatc gcaccggagg
    11701 ggacataaat atgagttcca ttcgtgtaga ccacactcaa caattgggat aagaagaact
    11761 cgtctgctaa tgccccaacg ctcattatga gcagatcctt cttattttcc acaacatgtt
    11821 tactaaaagt ttttgccgct gcctgagagg cagcttcaat aacgatgtcc gcatctttga
    11881 attcagaaga agtggtcaat tctgaaaagt ttgaaaatat agctacatta tctttacgta
    11941 atcgcaattt cagtttctgt gccgcatctt cgacggtatc aaatagcgct atcagcgtag
    12001 cgcttcctac tttaccagaa tctatggcta ccgctagttc gcttccaata gttccacaac
    12061 ctataaggca aaccttcatc agaaacactg attttttacg tcgatagaat cgtaatgcct
    12121 gtcattactt caggcttgtt aattttctaa cgtcttccgc ctcgctctgg cttcttctgc
    12181 cctggtggtc gccttttctc aaagcgttta ataaagttat tcttattacg caactttgag
    12241 acggggctaa gtcttacctt cccttgaatt tttggagtct gcccccttac cttcccagcc
    12301 ttagtcagag atccatgcgt tggcattgct ggcgattatt gaagctacat ctaatatata
    12361 catatgcaca ttcatctcaa cacagctata gtggcaagta ttgcctacga catgaaccag
    12421 tagggcaatc atctcctcga ttatactcat ttaaatgtgc tgtgagacat gtacgattat
    12481 gatgcttgaa gcagactaca aacatcgagt gctatccttg aagaaggcaa aaaacgcagt
    12541 gattctagca cataactatc agctgcctga agtgcaagat gtcgctgatg ttataggtga
    12601 ttcattagca ctctcacgaa ctgccgccca gtcagacgcc gataccatag tgttctgtgg
    12661 cgtgcacttc atggcggaga ccgctgctat catttgtcca gataagcaag ttttaattcc
    12721 tgatcctgac gcaggatgtt cgctcgctgc tagtattaat gcccgtgaat tgaaatcttg
    12781 gatagctgag catcatgatg ctgtagtagt aagttacgtt aacacctcag cagaagtaaa
    12841 ggccctgtca gattactgtt gtacatcttc taatgcggtt aatgtagtga acagcatacc
    12901 ctcgaataga gaaatactat tcctgccaga tatgtttctc ggatcatatg tagctaaaat
    12961 gacaaagagg aaaaacatgt acatttggcc cggtgaatgt cacgtacacg ctggtataaa
    13021 ggctgaagac attaatgaga tgttcaagaa tttcaacaat gctgatttcc tggtgcatcc
    13081 ggagtgtggt tgtacctcaa caacattgta tcatatcgcg actggagaat tggaaaaatc
    13141 tgcacatata ctttccaccg aaggaatgat gaaacacgca tccgcatccg ctgcaaagca
    13201 gtttgtagtt gcaacagaaa cgggtatact ctacagaatg aagaaggaaa atccacataa
    13261 acaattcata cctattaaga aagacgctat atgcaaatat atgaaattga tcaatttgga
    13321 aaaaattcaa aattcgcttc ttaataacgt gtaccaagtt aaagttcctc ctaaaaccgc
    13381 tgtgaaagca cgagctgcca tcgagcggat gctagcaata tcgtgactga agaatacaaa
    13441 ggggaaggaa gaaggtgtga gtagcgaact aagatggctg gcaatcactg ttgctcatta
    13501 tctctaggct ataatcaatt gcttttggag aatgggttag gtacccgagc gaaataatat
    13561 caggttgtaa ctttgcgtaa tcagctatgt tatgcggatc aatccttcct gaaatctcta
    13621 ctttgacttt tttacgaacg cccatttttc taatctgggt tattgcagct tttgcttgct
    13681 ttggagaaaa gttatcgagc attatgatat ctgaccctgc cctaaccgcg gagaccattt
    13741 cggccaaatt ctttacttca cattccaata ataaacaacc gttaattttt tctctcgcca
    13801 gtctgataga ttgttcgatc gagtcggcca ataccagatg attatccttt atcaaggcca
    13861 tgtcatcaag tttaattcta tgggtcactc cacctcctaa taatacagct tttttgtcga
    13921 aagatctcag tcctggagcg gtcttccggg tacaagcaac ttttacggat ctgttaattt
    13981 ttccaaccat ctcaacaagt ttccgagttt ccgttgcaat cccgctcatt ctcattatca
    14041 gatttaatgc ggttctctct gcttggagta tagcgagcgc gctgccagaa atattcatta
    14101 ctatactgcc atcttctaca cgagatccgt ctgtcactaa ggtctgaact ctgcacttac
    14161 aaatgtcgaa aaggattgcg gcttcctcaa gaccagctat tattgccgat tttccggatt
    14221 tacagataat ttgggcgcaa gcagagagat ctggtgagag aacgctttcg ctagtaacgt
    14281 ccccagtgcc cacgtcctgc tgtaagaaac ttattagcga ttctctaacc tcaagatatg
    14341 cacgtaaatt cttcatcttt cgtttttgtc acgattaagc caccttgagc gcgtatttat
    14401 gtggaactgt tatatcgagt gtggccgcca ccatagattt tgccggctca aaaatcagga
    14461 taatgccaga ccagtctggg cttagatcgg ccgacttaca aactggacaa acctttccaa
    14521 ctgtgacagc tttgcacttt ttacaagcta gttccctagc caattattta ttcccacctg
    14581 ctccgccttt cttttccttc agaggtgctg ccgccggtgg ctgctttgct ctaagcacct
    14641 cctgttcaat ccattcagta gcaccaagga agggttgtct acaggttatg cctatttttc
    14701 ccattgcagc tcccttccca agacttatgg ctgtaaccct tgcgcgtata cgcgagccaa
    14761 tttttaaact tttggctgtt tgattggcga ctatgacacc ttgtttaacg tcgcttctca
    14821 gataatcatc ggtaatctgc gacaggtgta acaacgcatc agtcggccca attctcacaa
    14881 aagctccgaa atcagtaatt tcgactactt cgccctccac cacttcctgc aactttggat
    14941 agaacgttaa tgccttgaag gttaccctat ggtaggtagc accatcgcct gccaccagtt
    15001 taccaactga attggcaata gcatcaatga tcattatgac gtatccgagc tcaggactga
    15061 tcatactctc gtatttttct tttaaaattc caatagctgt atccttcaat gaatttttta
    15121 atctactagg aggaattcta actacatcac tcatatgcac tatttcaaac ataatggatg
    15181 tacgcttgtt ggatccaagc atcaatttat gaaccttttg gataccttaa ccatgtccta
    15241 ttgcaaggta gctaattcac ttctaaggtc aaattttaca gaggagtcag tcacctcgaa
    15301 cagggcatct ctctcgatta tgttcttcaa ggcggatctc agatatcctt cgaagaattt
    15361 ggaacctttt cttgctaatc tgtgtctaat cactatgatt cggttgtcag cttgagagac
    15421 ctcatcatac gtaccgagat ggccatatcg gcagattatt ttttcaatgt actctaggac
    15481 tgatcgctcg gtaagctcct ttttccagaa cagtatcgtg tcccttgcta ccgtttcccc
    15541 aagttttagc ccaatagtgt atagactctg ttcatcatgt gattcgagca aggaggttat
    15601 gaactctcta tttataacta ccatgtctat tttcgatagg tatcgggtaa attctgcata
    15661 tcttttcaag atagttccta ccaatgcgtt aacggagata cccattcttt ctgcctcgtc
    15721 gtccaaaact ttagataaat ccagaccaaa tctaaaagat ctggttattg tatccgagcg
    15781 cgcatcagag cttgggatac ctcccaaact aatatcaacc aaatgatact cgccgaggta
    15841 ataaatagtg tggtgatttg tagccatttg tgttgatttc tgttcaagtc atgtcgtaat
    15901 tgcgaagatg tacacacgtt ccttattaaa tttcgtcatg gttggtgcct gtggcaaaaa
    15961 tatcgacaga cactactcag aagggctcga ttagttatct tgcgtattct ccgaccaatg
    16021 aaccgatgca tagagtacaa aaaaaagagg gaccggtgca agctagcgga aacgatcgcg
    16081 acatttgcgg tttatgtaac agcattgcga tccttttcga ttccgacact agcgagaccg
    16141 tctgtagcaa atgtggcgtg gttctccaag agaatgccga atcgttaggc gctgaatggg
    16201 gaatatactc aggagacgat atcgaaagca agagcagaac aggtatgcca acttcgttag
    16261 cgtttcacga tatgaggctt tcaacgttca tctcttattc aaatgtggac gcaaacggtg
    16321 gtgtgatcag ccctgaacag atggctaaga ttcaaagaat gagacactgg aacaagattt
    16381 cgagcaataa cagaagctat catagaaatc tgaaaaatgc ctttgctatt ttaagcactg
    16441 taaaagataa actgtcgcta aacgacgcgc ttatagaaaa atctgcctat aattatcgaa
    16501 aggctctgga caaacgaatt atcaaaggga ggcttatacg tgcactggta gttgcctcgg
    16561 catatgctgc atgcagagaa ttgaatgtgc caagaacgct agttgaaata gcacaaactg
    16621 ctaacgcaga cgctatcttc gccggtaaat gttacaggtt acttcttagg catctaaaac
    16681 ttcatttgcc gataattgat tcgaacgaac tggttacgat agctaatagt gggcgcgtaa
    16741 gcgaaaaggc ttaccgcaga gcccttcaga tgctttctat agtaaaggat atcgcatggc
    16801 aaagatctta acgcgcttgc tgtggctaat acgcctcgtg tctgaaggaa ggtgaaaagt
    16861 ttagccacgc acaaatcgct gtggcagacg acactagtat cgtaacacta agaaaaagat
    16921 ttcaagatgt gaggaagata tttccatgat ttatctttgt aattccggtt ttattctttc
    16981 gtatcgccac atattgtttc tagacttatt tcggttgttt tcgcttacat gccgagtgtg
    17041 tgaatgtcta acaagaaagt aatagcagat acatcgggga gccgaaaaag aatgcacctg
    17101 ctcataacaa cgttttatag cctgaaaagt cacaacacac catgcgaaat gactaggatc
    17161 aaggtagctg tcaaagatga gatcataaga ggtaagggaa aggagctgaa cgtaaacggg
    17221 attagaatag ctttattcaa cgcaaacgga aagtattatg ctgttgaggc tctttgtaga
    17281 catcaagatg gttctttggc accaggaaag gtagatggag agattgttga atgtcctcta
    17341 catacatggc actataatat taggacaggt gaactccttg attatcttgg aggagtcaaa
    17401 cttacaacct atgatgttga catcaaggga aacgacattt acgtagatgt ttagtatttc
    17461 ccaaatttac ttatactaga agagcagatc accggcatct atcaataaga gtattggcac
    17521 atgtctaggc agtacaacgc aaagaacccg aattgaatag taatccccag attagggttc
    17581 atcacattga aagtatataa cacgttgacg aggcgccttg acgacttgaa ctccgacgga
    17641 gctgcctcca cgagaattta tctctgtggc gtgacagttt atgataattc gcatgttggt
    17701 catgctagaa cgattgtggt attcgatatt ctacgccgct acctcctttc caagaattgt
    17761 cccgtagaat ttatccagaa ttttacagac gttgatgata agataataaa tcgtgcaata
    17821 caggagggat cgacagcgga agaagtcgcg tctaggtaca tacagaatta cttcaaggat
    17881 ttcaattccc taaacgtatt ggtggccgat cgatatccca gagctacaga gcacatacga
    17941 gaaatgatac agcttattag tgcactgatg aataaaggct atgcgtatct ttcgcctaac
    18001 ggaatttatt ttcgtgtaag atcattttca ggttacggca agttgtcgaa agaatcaatc
    18061 gaagacttag aggcaggagc tcgaatcgaa gttgatcaat caaaggaaga tccacttgat
    18121 tttgctctat ggaaattcta ttctgatcct ccagtatggg atagtccttg gggaatgggg
    18181 agacctgggt ggcatattga gtgttccgcg atggctttga aatattttga aagccctttc
    18241 gagatccacg gaggtggata cgatcttgtc tttcctcatc atgagaatga gattgctcaa
    18301 tcggaatctt tttcaggaaa acagtttgca aaaatatgga tacattcagg aatggtaact
    18361 gtcaattctg aaaaaatgtc caaatccttg ggcaatattg taacaatcca ggatgcacta
    18421 agcagatggg gacctaacag tatacgaatt tacagcattt ctgtacatta ttcaaaacct
    18481 ttggactact cagagaaatt actaaacgag tctaaacata aatggcgtca gatagagact
    18541 tgcgcttgtg aactacgttt tgcgattgga aatcagggtt acctaacaga agtggaacaa
    18601 ttgtctgatc agacgatgaa ggcttttgaa tctgcgatgg aagataattt gaatacttcc
    18661 ttagctttca ctgtattcct taaacttgtc accgagttaa accactatgc agcagaagaa
    18721 aaattgacag cagatatgtc taaaatcgcc ggcgtagttt tcaataatat catgaacgtt
    18781 ttgggccttc ggcttatcga agccaacgat atcgagaaaa aagaaattga aagtttgcta
    18841 agcatgcgcg atcaattacg agaacaaaag aaattctatg aatcggatgc gatacgaaag
    18901 aaattggttg atgaattttc agttgaacta atcgatcata agaagagaac catttggggc
    18961 atggttgagg gagcatcaag acctcatttt gttggctagt gtttatcagt aagtcctaat
    19021 tgattatatc gtttgtcttt cgggcaagca ctgaatattt cttccttcgc ctcagctttt
    19081 tcaagatatt aatcatgtta tctttcccgc agctaatgac acgatgtgat ttctgtcgcg
    19141 gccccgctga tgtccacttg cttgtggtac tactgatagt actaatgaaa acaaataaaa
    19201 aaattctcgt ttgtaatgta tttaaattcc gctcaagatg atcatcctac aacccgtgaa
    19261 tctaaaacaa aaacaaaaaa cgataacttt tatcgtatcg ggggtaatag caatgattgt
    19321 gatatttgcg tagatgtttc aattagaaag gcacaaaact tttcagattt atatgaaaaa
    19381 gttatttcag actcgccttc actaactcag ttttcgaaac gagataggaa atggcaatcg
    19441 aaacaatatg acaacgcgac aatgatcaaa attacgacag ttatatacca aaatttcaga
    19501 gactgcttaa catcgctgaa tcattacagc cgacgtgact gttacggcgt gacgagttat
    19561 aaactgtctg cgatgggttc cgtaacgaag ttaaaacccc gcgaattgca ggttcactct
    19621 gataactcaa gactcgacat attgggattt tactatataa acgtaaactt agagtcgcaa
    19681 actgcaacga aatcatttgt cattgttact ctgtatgaca aagaaggcaa ggtaatatct
    19741 ccaggaagag cacaaacaga acaggtaaat attacaacgc actctctgga gcccttcgga
    19801 atcgcagtga ctgaaaaaat tggagacaag attaaggact aatctcttgc ggctgattct
    19861 gatccctatg tggaagtgtt agaagatata gtatcaaccg cggctttagt tctatgttta
    19921 tcggagcaat agttgttata cacatgtatc agagcagatc gaagatagta gcatacgctc
    19981 agctcaatga caatcacttg tcttactgta aatgatttta ttacacttac acttctcgca
    20041 ggtagtagtg ttatacttgt gtgttgggta taaataggaa taagaaaaaa ctcaaatgga
    20101 aagttgatta tgagaatagc ctgtacaaga tatatgattg ggacaaaaaa ttagcaggtt
    20161 acttcttccc aagatatgga tccgttgaat caggggaaac aggcgaggtg gatgatgata
    20221 gtgatattgg tcatgacgaa catgcagacg aactaaataa atccaaagca aaggtatcgg
    20281 gaggaaatct cctggttcct atgttgaagc tcaatctact agatgtacag gagggaatag
    20341 atttggatta tactattgaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    20521 atttggatta tactattgaa tcactcgaga caaacttgga aagaacgaag ttatggaagc
    20581 gatggattgc tgaaaatcat cgagaatcga acatagttgg tagtggcata tatactgcaa
    20641 gagaagatag aaacatgcta tcaatagtcc tcagcatagg ttcagatttt atactaggcg
    20701 agatagaggt tataacaaaa ttagccccct tacttgacaa tcttcacgag tccggcctct
    20761 tataactcat caaaagagtt ataagcacta ccttttttag gatttaaaaa gtggaacggc
    20821 agagggaata gtaatgacga cgataatacg aaaatgaacg ttattgatga agtatgaaga
    20881 gttcaaatag tgtgattgat tatcatctcg atatgttgcc aaagaaggag atgttactgc
    20941 gcaagaatga ggagactacc aagaaggaat gtccattgtg cggatgttcg cttccgacaa
    21001 ccagcgaatt aaacctttgt ccagaatgta taaagagcgc cgattgctgt gttggactag
    21061 aaaaaattcg agaataactt ttgtagtgtt atagtacgga tatatctatt tgtattcaaa
    21121 aaacgtgaaa tatagccatt ctctttagaa gtgagcaatt ttcatttttt ttcatactgc
    21181 aggtaaccat gcattcctca taaattcgta attcgacata tcctctgaaa aaaagcagat
    21241 atttatgaaa ttcttcgttt ccctgcgact ctggaattca tgtatccgat gcgagagaag
    21301 aagctataat attgctgtag tactgcatat aaatctgagc gcctgatgaa taaggatagt
    21361 tcgtttcgtt cactctccaa gcctgtatca tcgtcgggcg ctcatgatct ccattagtta
    21421 acaaggtctt ttagcagtgt gaacttattt gagtcgacca tttacaattt tttcttttga
    21481 gctcagtaac aataccctcg gcttagacta atcgacaatc cagataatca ctatcccttt
    21541 agcgtttgac tctatcggtg cttaaacatt gccagacatc tattgttcat atgtcaagac
    21601 acttacgtga ttggtttggg aataagattg gccataggac tagcaataag gttagtacag
    21661 cagcaggatg ttcgtagtac tggcagtcac gatgctaacg gtggagatgt taacgacgct
    21721 aatggtactg ggagtagcga taataatcgc caaagatgta atgcgcgttt cgtgtttagt
    21781 tcatttgtaa acctggcaga cttaggcaga gatcgtacga ccttgcttgt acaacgaaat
    21841 aattatgatt gaacccactt atgatacaac cgacataagt taagttcctc gtagatacat
    21901 ggttctgatt tcagttcaac aaagatgaga attataagta gaaacgagaa atgcagatca
    21961 attctagaga cagcgaatgt agaaggaaca aacctaatcc ggattatatt agaacatgtt
    22021 ctaagaccat agcggcttcc aaaaccaatt acagtgatag tgactgatat tataaccatt
    22081 atcggatttt ttacccccct gcagatttga gcaaaatggc tgctcctgga gcgaattcaa
    22141 aattcatgtg gggcgatctg gttgcaaata gaatggactt tctaataatt gcattcatag
    22201 tatttcttgc atacaagcaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnatagtatt tcttgcatac aagcaaggag actgagcctc cagccgccga
    22321 taaaaatagc tgtaacaaga cgtgccacgt tctgatcgcg aatttatcac aagtaatagc
    22381 aacagggagt gtatagatta gagttggtgc tttatgagta ttatgaatat tgaatattgc
    22441 actgtttgga agcctctcca tgtgaaatac agaggctcta ttctattggg cctctcacat
    22501 agttgggtgt caacactggt aacatggctc taattgattt atgtctgtat ttcatttgta
    22561 tttatttagt tgtccctctt tatcaatatc ttctcttaga aactttatca taggataggg
    22621 tactggtaag caaaaaattt caatcagtat ttggcgtgcc tagcctagct caaagcagaa
    22681 ttgtttacga tagagctgcg aaacatatca atcatactcg tacacgcgta gagactcttt
    22741 agtctttagg aatattcaat ttgataatac atggtaagca aacaacttcg cggataactt
    22801 cggatcgatg aaacccagaa tggcgtgaac ctatatggca tgattatcat accttttatt
    22861 atgactaaac cgcatataaa cctccattat ccctttatta ttattctacc attttcaatg
    22921 tgtctgtgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnntttt caatgtgtct gtgagcgtga taatccctac tactgcaccc ttttcactaa
    23041 ctagaacaca ctttgaaaat ctgaccaaag gaaccagtgc cttggcagga gttgataaat
    23101 caactagagg aggcggtggt tccataatca tggagatcgt catattgcgt ccaccctctt
    23161 tttcagcttt ttccaccatg ttttttgcca agccgtcttc actcacaata ccaacaacac
    23221 gggagccgtc gaacactgga atttgactga tagagttact tctcattcta tcaatggcta
    23281 tccgaagggt gtcgtttttt tgtactaaaa ttagcctacg actgcaaata tcgccagctt
    23341 tcatcgaaga ttggccttcg agagagctta agagctcgaa gattttccgg gcagtctcat
    23401 agcttggttt gcagcgacct gactctatct ggttgatcat ggatgtgctt attccagata
    23461 gcccggccag cttgcgctga gtcaagccga gtctaaccct ggcctgttta acatattcaa
    23521 gtctcggaag catctccatt attttttgat ttcttacatt ctcatgttta ataataacat
    23581 tggcttaaag taagctaatg tcaggaatct gcgagaacga ggcaccaaca aaatattacg
    23641 actcatgcat tatcatcgag acaacttcat tttttgtctt tcctgctatg ttgttgatat
    23701 ccttatatta tgtgcgcacg aaaagtctct attagcaacg atgaataccg gcagcagcag
    23761 tacgcaaccg aagtgggatt cggtgaagag atacaagtta accaaaatgt tgaatgaact
    23821 tggaagcatt tcgggtcacg gaaccgagct tgtaactgtg tatgtccctc ccaggagacc
    23881 gattttcgat gtcattgcac aattgcgtaa tgaatcaggt actgcttcaa acattaaatc
    23941 cgatttgacg agaacccacg ttcaggacgc tttgagcaga acaatagagc acctaaaact
    24001 tttcaaggag acgccagaga atgggctcgt catattttgc ggagctattc caagcgggaa
    24061 gggtttcggc acagaaaaga ttgaacttta tcctgttcta ccaccgaagc cagttcagat
    24121 tcagctttat cgctgtgacg atcatttttg gatagaccac ttgagggata tgatgaagga
    24181 tgataaagta atagggatta ttgctattga tacacaagaa gctggccttg gaatattgac
    24241 tggtgataga tgggtagttg tagacacaat gacttctggc gtcgctggca aacacaggca
    24301 aggaggtcag tctgcaagaa gatttgaaag gcttcgcgat aatgaactaa atgaatacta
    24361 tcacagagta ggagatcatg ctcaaaaagt gttcattgat cagtttaatg ttaaagggct
    24421 tatagtaggg ggacctggtc caacaaagga gaattttctt agagaagaat atctggatta
    24481 cagactgcag aacaacgtta ttacgacttt agacacttct tattctggtg acgaaggcat
    24541 tcgcgaaatc atagacaagg ttaatgatca aggtgttatg acagaatatc ggttgatgga
    24601 ggaaaaaaag atagtcaaga agttcatggg cgaagttcat tccggaagag gtcttggtat
    24661 ctacggtatt ttggatgtta taaaatcact ccagaacgga attgtcgatt tggttattgt
    24721 gactgacgat attacttttc taaagttgga tgtaaaatgc aaaagatgtg gcactataca
    24781 agaaaaattt attgagagaa gtaacttggt atctaccaaa caagagcttt tatcaaaacc
    24841 atgtccttca tgttcggcag tcgattatga aagcagcgag aaagatattg tagatcatct
    24901 agaagaatta gcgactatga cagcaagtcg gctggaaata atctctggaa aatcggaaga
    24961 aggtgctcag atagctagcc ttgggcgaat aggagcaatt ctccgcttca ggcccagtag
    25021 tagtaatact atcgctcgga tatcttgata tttttttcta agaattattg ccaatatacg
    25081 tagtctctcg atatggcaag aactggcaga cagggaagac agattcggaa ggtaatgata
    25141 ttggaggatg aacaggatat cctccacctc tacaaggatt atctgagtgc aaaaggtcat
    25201 tctgtaattg tgacctcaac tatggctaat tgttgtactt ccagacatgg ttg
//
LOCUS       DAOR01000252           14958 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_47766, whole genome shotgun sequence.
ACCESSION   DAOR01000252 DAOR01000000
VERSION     DAOR01000252.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14958)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_47766"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4582..4753
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11811..12076
                     /estimated_length=266
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4230 a         2858 c         2805 g         4627 t
ORIGIN      
        1 agattttgga gagatttagg atttgtattt ggtgcagtag ggatagggtt tattgcagac
       61 atatcgagta tcagtattgc aattcaattc gttgcatgga ttgcactagc atcgggaatt
      121 tttgttctta ttgtaatgcg tgagactaaa cgcacctcat cgatgactat gaaaatatag
      181 cccatgaatt tgcaacctaa gtaagcagat aatgaaattc cgccaagagt tgaatatcga
      241 ttaactacta aaggacagga acttgtggaa tctgttattg ctatgctaga atggatgaga
      301 aaatggtcat caaaggcaaa gtctaaaaag tatgtcatga aatgctccga tattagagta
      361 acactcgcaa aattcaacca tttagtttga gatatagtat caatattatt attcctgcag
      421 gatttcgttt tagacaaaag gtgttggtct gattattgaa agcagaaaat gagttgaact
      481 catatcttag cgcattagat agaagttgtg ttgatttctc atcctctatt ggactaccat
      541 tttctaaaaa gtttctgaaa tgtctgtagc tttctaattc aaactgaagt gatttgatat
      601 accattcttt agcatgaatg taattttcag tagcaggctt caaagcttct actccaccaa
      661 ctgatttctg aaacattggc aagtaattgt cggtaactga catcattgtc ttattatcgt
      721 attgttttaa gtcttcacac agcggttgat aacgaaggaa aacttaaaaa tagcacttca
      781 agtttttcag cttcgagatg tttcttttag aaggtgttgt tcttgttctt gtatttttat
      841 cagtattagc aaatgacgtc tactagatgt attcctatta cccacctgat tttgagtggt
      901 atgttaactt cgtaactcca gggaaaaatt cttgtccaaa gtgggcctat atagtgccgc
      961 ataaagttcc attgttttta aaactttgca gaggaataca aagataaatc aggtgtgatt
     1021 gctagatctt ataaggatgt agagcataat caattgtaga ccgatgtaaa cacaaccaaa
     1081 cacggtcacg gtggttatcc atgaaaaata gaaagaacat atcaaagcgt aacactttgg
     1141 actcgattat ccatcacaat tttttaagcc tgaacaaggc ttttcaattt cagcctcctc
     1201 gtctggaagg tagagcatac catcaatttg ttccagctca gattctatct cgtcttgtgt
     1261 tggttttgcc gccatttctg ctattttttt aatgtagagt aatagcttct gtgttgaatc
     1321 tgggaggatg tggaacttct cagcataata ttccgttaaa ggtttaatgg cgcggttata
     1381 attgcgatct ggaaaaaact cttgtaactt gtctagtata aattgtgcga actttgaggc
     1441 tctaacaact ttaataacac tatcaatttc tattcgtcta gcccgtcctt ctataggttt
     1501 cggatccttt tcccagatgt cattgggact tattcctgtt aaatattcta gattgttata
     1561 aatatactca tatcgtttat atccttgacg attcaagtcg cctggattgg ttagaaagga
     1621 atgatatagt cttgcatatc gtatgatcgg agtgattaca ttcttacccg gttgttgctt
     1681 ctcgggatcg gacgcttttg gatatggctc ctctacttct tgctgaaact gttctagtct
     1741 ttcattatag agctcttcat cgttaatttc gtcttcaatt cctttttctt gaatctcttt
     1801 tctcacgaaa taggctaatg tgtcagcatc aatgcctaag cgtcttactc tgtcagtata
     1861 tttaggactc gctagctttt gcaattcata gtgtttaccc atgtaatctt catctgcctt
     1921 taactgttct ggaggcgctg tgtccatctc ggcaatagta tgaggtaaat ttaaaaccaa
     1981 atctttagta gaaatttttt tcttatacgc atcgttttca ctctctatgt cctcggagat
     2041 gaccttttca gcaatatgaa ttccagcgca atcaaaatct gttaaaattg caactctacc
     2101 gtcctgttca ttaatttctc ttatcaaatc tttaggatat tctgcaaaat gaccttgagt
     2161 attaacaaaa gcgatgccgt agtcgtcacc aatgtatttt acgatttcaa gtgctcctct
     2221 cttttctatg aaaaccacat ctgtgccttt acccgctaag gatggtatat catcaatgtc
     2281 cacattgtgc caggtcccat cgaagtaaag tgttgctcta ggtgatgcga agatggctag
     2341 ttgttcgcgg gttggattac cttctaagtc actttcacag atttcgctta tcttctgtgt
     2401 catgtcatcc cttgtgtttt cttttataac cgtaatgtgg tatctttcct ctattggcaa
     2461 aagatactct tctcttaaat gataccaaac tgtcgattgc gtccagggat cataatcatt
     2521 actattatga tccaatattc caggattgcg tctgacatca tcccaacgct tttctaataa
     2581 ttgccttaag caacctgttc tatcaggttt gtatttacct gggctatacc tagattttgc
     2641 tggtattttt ctaactaatt cttcaatcgt ctcagcaatg tcatgtgaga acggtgtaag
     2701 tgtcaaggct gattttccat aacttatata ctcgatcctt ggagcagcta aatgagcaat
     2761 aataacgcat ggcacttttt gtttagctat cggtatgctt gcctcatttg agatattttc
     2821 acctactgct gattttctta ttatttcaat aatatcggat gcctctagtt cttcttctgt
     2881 ttttccatgt gcccattcat acgtattagt atactggcct tcaaaatatc tcttgttggt
     2941 tatcgatgta gaataattga caccgctaat tactatcgat tcgtcactgc ctgattttat
     3001 aggaattgct agcacttcaa aaataaaagg aaattggata ttatcgtctt tataaattgc
     3061 atcctttgtc ttggagtata ccgctttatc actatcaatt tcaatatatc ctggtttaat
     3121 ttgttcatac ctggcaagta gtgctgattt ccttattcgc ttggtatcat ttggaacaga
     3181 aagctttgac acagggagca tgctcttcct taaagcactg tgaagttttt tagccctcga
     3241 aaatgttaat tgttcaagca ttactgagtt ccaatcatca aacctaccat cgtgctggtg
     3301 gatttctcta aatccactcc tgttaatggc attatacaca gatatatttt tatcaaatat
     3361 atcattgaga aaatcgttca ggtccgtctc actgtaacaa taaacgctat tagagttacg
     3421 atcgtatctg taaccgcaag ttgtgcaagc tgcaggcgac tcgattatat ggttaagtgc
     3481 aggtaagtct atttgtaacg cctgatcgaa ataaaatttg tatgatatat gcgtgttgaa
     3541 caaagtatat tctttgcagt aattcttaaa tcgcttgtaa ttattttcat ctaatgctgg
     3601 taatgtaagt ataacttctg tatccgtgct atcacagtat tcactatttg taaattttac
     3661 ctttatttct ccatgcttcc tatcaacctg aaaatatgcc ttgtctattt tgttattatg
     3721 ctgaataatc aacggttcct tccattgttt atctatagaa ccctcagcgt taatcagggc
     3781 gtaaggtatc gttcccatct tctttatcgc atcaccctgt actcccctcg ttactcgata
     3841 ccggctgctc ttgctgctat aggctcgctt ataattaaat gcctcaacca aattgggaaa
     3901 cacaggtata ttctttgaat tcgaatttct tactgctatg cgaaaccaat cctttgagtg
     3961 tgttatagag acaataatag tgacatctgc ataatatttt tctgaaaagt cagcagcgtt
     4021 atcaagtaat tctttgaggc tgaatggatg gcaatctttg gcctcaatac cggtattttt
     4081 tgtaatgtcc tttagcctta cgtaatccca aaaatttggg tctgaagagg taaaaacctc
     4141 gttgggtata tcattttcta tgtccttagc accatttttc tttgctgtca tgatcttaat
     4201 acccttgagc aggacatcgc gacacttgcc ctatcaagtc cttggtaatg ttgagttgtg
     4261 ttagctataa tgttgagcga tacgagtaca tagaacatgc ctcttagagg tgaagcaatt
     4321 tcttgttgat agaatgcaaa taatctctgc attacctttg gtatggcatc atctttccat
     4381 cttatccgcc cttgttgatg ggtcttttta gctagcattt cggtactctc cagggatgga
     4441 tgaaaaaaat gagaaaagta ttcggcatga tagaatttca atcaatataa tttgctcctt
     4501 tttcctgatg tcatagaagg taggatgcga tccaagaaac ttggcaaatg atatgaccgc
     4561 ttttaagttg ttgttttgat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnncgctttt aagttgttgt tttgatgatg ttccgaagac ccgttctcct
     4801 tcatatatat ggaaaattcc tgtattatag tagaattggt aggactgggc actgaggaaa
     4861 ttttgttaat tgtaatagta agttttgttg gcatttactt taaacctcat tttccggttc
     4921 tattatatcg tatattaccg aactcaagta tatgaaccca tgtacctatt ggggtcagtg
     4981 gggtgtgaga catctatgat aacggtattt ggtggttcat atctcattgg tgctgcataa
     5041 agattctctt cgtcgctatg aactaacttt attggtagaa tagagcttta ctggtcccaa
     5101 tgaatcagtt acatgagaac cacactttag gcatgtgggc aataaatcaa cgatagggtg
     5161 tataaacgtc tcctctactt cttataattt agctatgcat aaaccataat tccggatacc
     5221 aaaaacgttc ccgtttcgcg aactggtttt agtctgggga gaaagaagaa tttggttaag
     5281 tacaacctaa cagctatata gtatatgtga tacttcttct tgtattgtct actaataatg
     5341 gcttttttct attgatgttc acatttgtca ttatcatgat catgattatt ttaagactta
     5401 gaaaggctat tcaaggaaca aaagtaaatg taaagaggac aattatcttt tcagcctatt
     5461 tcgttgctat ttcttccttc ttagtgtaca attctttttt aattggtagc atccctggtg
     5521 tatatgttat tccttatttt gctgttgttc tggctgcggc atattgctct tatatatatt
     5581 ccaagagaac cctttctttt ttgaaattac ctgatagcga tactgatagt tcagctatat
     5641 atgtaaaagg tggattatca atatattttc tatatgtcgc agcattaact atacgtattg
     5701 caattaactt tctctttatc ggttcagaga aattttattt taataatcaa caaacaatat
     5761 taggaaatgg tactgctacc gttattatgc ccatgtttca cactgatcca gcaacgacga
     5821 tattggcttt tacagttacg gacttccttc ttcttatcgg tgttggtctg attataggaa
     5881 gaaatacaag agttctaaaa tattattatc aacacaagaa gagaacgatg tcttaagtta
     5941 gcgtagagat ctgataacct cagtttagca attcgaaaaa tctctaatag attataaggt
     6001 acacagtata tagtacagtt cagtgggata aaataggata attcagaaaa ctgcgtacaa
     6061 ttgtagtaaa gatggtacgt agaaggttaa tttctgccat gttagcgaca gcaacataca
     6121 ttcattcaga ctgtccttcc acgagtggtt gtaaagaacc tatcagagga tgacccctaa
     6181 tgcttgaact cgagcgacaa gtaaattagg ctagataatt tcgttccagt ttttttgttg
     6241 cagtagctac tagacaaaca ggcttgtaag tctatagata aattgatagg agccctcgta
     6301 tcaaatgaca accgcctgaa tggtccattc gaagaaagct gaattagaag taaaaattga
     6361 agttgatatg ccggttgata ttattgacga taaagatagg ctcaaagaag ttgaaaaagg
     6421 attagtccgc agtatttcta aaggtctata cgaacaaggt gtatcattca aggttgataa
     6481 agttaatttc aaaatcaaat agtatttaca gctaccagtc tgctagatat atcgaaaaac
     6541 atcggtttac attctctacg taagaaaaac taaggaacaa aaagtgctcg cattattaaa
     6601 aaaccaaatg acccagttgt gatagaatat gtaagtgacg ttaatgtaat ttgtctaagc
     6661 ttttaaccta tccaattcac gctccatgtc agtgaaaaat actccagcta caggattaac
     6721 tatcaatctg aacttcaaac catcttccca tttaacctgg atatttcttc aaggagcttt
     6781 cccgagatat tattgaatct gctctggact tcagctttat cttttccttt ggaaactatc
     6841 tggacacgca tttgtggttt tttattatta ttatcgctag tatatcctag tggatgcgtt
     6901 ttcagataaa ttgagtctgg gggatttgat tcaactatct ctgcgagaat aggcgagagc
     6961 attgcttcgc ttacgcctat tgtttcgtat cttgattcga caatatggaa atctccaata
     7021 attttcttca tttgaggaag tacactttct aaaaatattg ctttcatctc ctcaggcacc
     7081 cctggaagac atacaatttt ggtctcaccg ctctcaatta gcacagatgg ggcgctgcca
     7141 acagggtttt gaattggatt tgatcctatt ggaatctttg ccatctttaa gcgaatttca
     7201 tttagttcat aagtcaccga atgtcgcgca taactctttt tcaacatgtc tagtgctgtc
     7261 ttgtcaagag cgagttccaa gcttaaacct ctggcaacac cttgcatagt tttgtcgtca
     7321 taagtaggac caaggccgcc agagagaatt aaccagtctg gttttcgaga aatagaatct
     7381 cttactccca aagatatttc atccacatca tctctgattg cagttatcct tctcaccgat
     7441 ccgccagctt ctgtaatttt ctgagatatc caataagcgt tcgtgttgat cgttataccc
     7501 gatagcaatt catctccaac gcataatatt tcggatgtaa acattctcat ggcaagctat
     7561 tagagatgag agaatgagta atattaattc aagccatggt gtgagttaag ctgataacaa
     7621 atcatgttcg caagtgttgc taaccatgga tatgataggt agaacaaaaa taaaacgaca
     7681 gcaggttgca cacaatctct gtcaagatga tctcatccgt gtttatgctt gggtgatttc
     7741 gcattcagaa ggaagggtaa acaaccacga tacgaacaac accagcctgc gttctaactt
     7801 ttgtatgagc ttagcccaat agttaattat cagtggtata tgaggttcaa tttgtgcctg
     7861 aatttcacag actgcgttta gatgggttaa tgtagcgtgg atgcaatatc acaattatga
     7921 acaattttgg aagcatcgag ttgagtgttg tattcctatc tgatactatc atgcatcttt
     7981 ggacgatgag caagtaagga tgagaacacc cacaagtgaa gtaaacgttc cgtcccccaa
     8041 acttaacgcc aaatcaatca tggcacttgc gctaacaatc tgtatttctt gcggcgggtt
     8101 cgtaacagta cagcatgcat atagtgaaag cccgataaag gtaatagcac agccaccatt
     8161 tacgggctct gacggcaagc taaacgcggt aggtatcgtc aagaatagtg gcaacatgcc
     8221 ggtggaggtc aatcttggtt tgaatattgt cgctagagac agtggtcttg ttacaactat
     8281 taaagaacca acctatggaa gagttattta cccttctaat gtttctccgt ttaagtttgc
     8341 agttgaacct agttggtctc tgtcgggagg tccctttatc cttaattcaa aggaggtctt
     8401 aacaccgtac tataaagttc taagtttgag ttactctaac atgcctgtag gaaatgataa
     8461 ggctcttatc ggaacagcaa aaaacgttag tcctgtcgat cttcataacg taaccatctt
     8521 ggcatctgtt cacaagacca acggaaccca gatcgattcc gtgaaaagtc agttatttcc
     8581 tgtcattagg acaggtcaaa ctgtagcatt tgctgcttta cctgatccag caatcaagtc
     8641 aaatattgcg tatttcagtt gtgcagacat cgatgttggc aacaatagtg cattgaacac
     8701 tttagggata ggtaacggtc aatttatagc gtatcagatg agcggcgtag tgcaaatcag
     8761 tgatttcaaa tatgacaata caacagacag tctagtcttt ggaataaaac attataatcc
     8821 agctggcgga ccatttagct tgaagatacc ggagatgtcg aaaaatcaac cagtatctgt
     8881 catattggat ggaaagttgt atactgaagc ttcagtaaaa atggatggaa agacagtacg
     8941 tgtagacttt tttgttcccc cagataatca tcaagtacag attaaaggta ttaggaatac
     9001 tgcctaattc cttgtcgata ttggtgtcct agttttttcc ttctgcaata ttctctaacc
     9061 atagtcccca aaaagcctcg tcttctttgg atgatctcaa ttggtttggg tgatttttac
     9121 ttgcagtggt cgtggttgtc acgatagccg acatgacagt gttaagtgcc ttcccatggt
     9181 cgtccaaagg aatgaagact agtttatcac aaacgtttgt acactgctca tacggtgtgt
     9241 acgaaaggac atttgcagga taaatagaaa tagaaaatta gtagatgcaa aggcaggttt
     9301 aaaacgaatt aaatcaagca aagagtaaag aattcttggc caatgaaagc ataagcgact
     9361 ctccaaactt agcatttcat gggtgtcagt tcagagactc ctttaagaaa aattgtgtat
     9421 atagaggaat ttgacaaaca cgacaccggc ggcaatttat tgtgcagaaa tatcgattgc
     9481 aaaaatcatc ctcggtcacc atacaggaag tactgttcta agaaatgtaa taaagatttt
     9541 cgtcgttggt attatcataa tttttattgg gaccgagtga gatctgacat tttcaaacgt
     9601 gataattata cgtgtcagat ttgcaaaaga aactacccgt acagatatcg gagaaggttt
     9661 gctagatcca aaaaattaga gtgcgatcat atcgtaccta gatcgcttta tgagcaaaat
     9721 ggatataaat tcgataccct tgaaaataaa attagagcaa cactagaatt ctttcataat
     9781 cataataatc tcagaacatt atgtcatgat tgtcatcgaa gcgtaacatc aaagtatatt
     9841 agcattaaat tcagagcgtt gaaacaagtg tctagggatg atgtaagtgc gttgctagcg
     9901 gaatagtccg tcgtagagca gaagtggggt agctgttttg aagtatggat tcggctatta
     9961 aatttgatca aatttagatt tatgaatgcg ggtatttttc gtttatgtta tctagcggta
    10021 atatttcgaa tattatttac ccgacggagc ggcattttta atgataagca atgggctgca
    10081 ttataataac tgcacctcgg agcgcactgg atatgcacaa aaaatgattt atttatggtg
    10141 ttttctcaat atgttatatg aaatgcccta cttgccagac tgacttccat ccacacatga
    10201 gtaacactct agttggtaaa aatcgaaata acgttagtgt cttcatctat tatcagctgt
    10261 gtcccgaatg caaagacccc gtcataggga ttattgaagc aactaagggg gaagcatatc
    10321 tcagcccgaa tgatattaat ggattaattc tgttaaccag ggagaaaaga acgtaacgta
    10381 cgacgcataa atagcgagct aggtggcacc tcttatcgca ccaaacacgg agatccagat
    10441 cttgagagtc tactccatca gggggtttgc cgacggttga agcatcgact tcttcactct
    10501 tatatagaag tttatgaaat accgtctatt gaaaattccg cctctcaaaa cgatagagca
    10561 gctcagaaaa aataaggatg aattgtttga atatttacta gactacgata gttcgctaaa
    10621 atcgacagat tctatcgaaa tcctagattt tgaatacgga gtgaagattt tgtattatca
    10681 taaggaagga agcaaaccat acaagactag ggtatatctc aataaaatgg gagataccaa
    10741 gctgctgaga tgactttgaa ttttatagaa tggtataatt tcaacttctt gcattaaaca
    10801 atcgtgtctt gaaaagagcg aaagtgcgta aggagagggg cgcattcata gatgaaatcc
    10861 aagattggtt tcgccaacgt aatagctttt ggacacttgt gagtttcgct tgtcttgtat
    10921 cgataatcat tttacagatt ttggttattt tgctaagcgg aataaatcct cacgttcgaa
    10981 atggaattta ttcaattctg gtgcctttgc tagtattctt cgtcgtgagt atcttttggc
    11041 gaggctcgac tccaactact ctatcgcttg taggtgtaat gtgcttgtat gttggtatgt
    11101 tttttgtttt tgcggagctc ggatcgctta aagttatgcc tcgccaaatg gccaacagat
    11161 taggatatgg tattatgcat gaagggcctc ctgtagatgc ggtagcggat ttttatttct
    11221 tgatggggat ttttgcactc gtattgtcta tggctgtagc attcagaccg tctatattta
    11281 gggcaagagg aactcctaca tgggtaccat accctgtatg gaccacggac aatgacccaa
    11341 aactgtcgta tggcaggagt gtcatgagac taatccctgt gcaaagttta ctacggtttg
    11401 ccgaccaaca tctagttgca aagtataggt acattcagat tttgataggc ggaagaatat
    11461 attttgtgtc accagacgag ggttccagaa ggttccaatg tcattagaga aaaggagtct
    11521 ggctcatttt tggggattac gaaagttcct gacggattca atatatggta gatactgagc
    11581 gtaccaaaca attaaaggaa gggtttcggc tttagctcac gccattgtcc acgagcccac
    11641 aatccccatt tttgcttatc ctcagatgaa tagtagcatt tctcgttgtg tggacaatcg
    11701 ttgcaccttg ctgaaggctc aatggttggg gttatgttgt ttcttaacaa cgtgttaaga
    11761 atgcgtgctc ttctcaacgt ttctttgaac agcttttcac tcttaggaag nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnntctg gcaactctga gattcttcga aatatcatca ctacatcatc
    12121 ttctacccta tccgcgcgtc caactagctt tacattagag ccaccttcta ccatgccttc
    12181 caccggtctc tctaggacgc tcagcgctcc cttttccatt agagcagtta tcatctgctt
    12241 gtgggtacgt tctgccggct ctttcctgtc aaggtatcca cgtctaaggc acccgcttgc
    12301 ttcttcgact gatatcttac tctcatcgcc caagttggga ttgagatcgg caaaaacctc
    12361 ttgtattaca tcataaacat caactttagc gggcgcatcg cttgcagccc tatagttttc
    12421 aagtcctacc tttatttttt tcctgacttt ttgtatgatc tgttcttcca tggctggtag
    12481 tttcatacca aatcagaggt gcaatgataa aagtttttct acagatctgt acaattttta
    12541 tgatactaag tgaatcgtaa cgcctgtgaa taaaacagcc aattgtatta gaagtcttcc
    12601 tcaggtgcaa gagcaattag cttacccatg aacccctaaa gttgatatca aaattttgac
    12661 gatcgaacct tacttccgtc gcctggttgg tcttttcttt gctctccacc tgtcatatcc
    12721 tagccttgtt gcggcaaaca ctcccagact gaagaaaaaa ccgccaataa acaaaaataa
    12781 aatcgagcga gtatagagac cccatgcaaa tccgagcgtt gcgctaacga attcagagat
    12841 tacaaatact agccacctat ctgccaacga tgaaatggca ttatggggtt cttttaactc
    12901 ttttctcaga atggctcaaa cagcgtgact tcgccagata aataaattaa catatccctg
    12961 aagatttggt cttttcatga gatacgttga ggttattttt tgtaatataa agtatatgca
    13021 tttggcatca tcgcctatca aaatcaaaac aaaatcatgc gaatcctgtc ttaacattta
    13081 aagacgagaa atccaggggt acagagattt tcaggggatt cttctcgtcg tggtctcgtg
    13141 gactccccta ttcctaaatc ccaactaagt tactgatacc atcaagaaga cgaatactcg
    13201 ccctttctaa attagaataa cgataccgca acactccttt ggaatattgt attaggcgat
    13261 tattggaacc aaaagataga gaaaaataga gcgctggttg ttgttatcta ttacgaaact
    13321 gactcgctat acgctctttc tcctacctgc gctaaatcca accctatctc ttcttctctc
    13381 ggagttactc taacgccgcc aggccaaacc caatccatta ccttccatat tacaaatgtt
    13441 attccgaaag accaggctgc agcggccgct gctcctgtgg cattaataac taactgcatc
    13501 gggtttccaa agaacgctcc attatgtccg cctggattga cgcgtgtctc gctaaatgct
    13561 ccagttaata gggcaccaac tacaccaccc atcccatgaa cgggccatgt atctagtgca
    13621 tcgtcaatct ttcttctatt cttgagtatt aagcaataga agcatacggt tgcacatgca
    13681 aatccgataa taattgatgc atagacgccg acgaaacctg acgccggggt aattgctact
    13741 aagcctgcta ctgcgccgga acaagcaccg acagcacttg tctttccagt gtgggcccag
    13801 ctaaggaaca gccaccacat ggctgcgaca gcagtcgcta tattggtatt ttggaatgcc
    13861 tgagttgata agaaacctgc agctccagaa cttcctgggt tgaaaccaaa ccagccgaac
    13921 catagcaaag ttgtacctaa tatgaccagt ggaatcgaat gcggctccat aggcgcttta
    13981 ccataaccca atcgccttcc tagaaacatc gctgttacca accctgcaaa ccctgaagta
    14041 atgtggataa cagttcctcc agcgaaatct tctgcaccaa gtgcagctag ccaacctaaa
    14101 tgcgttccct cacttcccaa agaccaattc caatgtcctg caaagtcata aacaaatgta
    14161 gaccacagca caacctgtat caggaacgcg ctcatcttga gccgatcagc atagcctgct
    14221 atggctaacg caggcgtaat cgcagcgaac attagctgga acatcacata tgccaaatgt
    14281 ggaatgctcg gagcgtagac atcagatggt gcattggcga aaacgttatt cagtcctacc
    14341 cagtttaaat taccaatgaa accggcgcct gttgcatctg gcccaaacat aatagaatat
    14401 ccaaaaacca cccactgaat gctaacgata gcatatacca aaaagcactg caaaatcact
    14461 gttaatgaat tttttctcct agttaatcca ccatatagga atcccacacc accgggagtc
    14521 attatcataa cgaaggatgc agcggcatac atgaaggtcg tatctccagg atctatacat
    14581 ggtccatagg taccattacc aggaggagtc aatgccttgt tacaatggta ttcgggtgtt
    14641 gttgccgggt taggaagcat gtgaccagtg ggcccaagcg ggccgaactg gccgaaggcg
    14701 ttatgttcac cgaaagcaat aagccctgat gttccgacta ctagcccggt tatgataaat
    14761 gcgaatttcc atttattctg ttcaaacggt aatgaaatca aattcattgc catggatatg
    14821 ttacatgcaa tccattcgaa ttgattataa aaaggttatt ataatataaa ccgtctttta
    14881 tgatactatt ctaatttgaa aaaatcgata cttcattggg aggtccttgg tgcctaacta
    14941 aaaagtacga aattgaat
//
LOCUS       DAOR01000253           16950 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_2982, whole genome shotgun sequence.
ACCESSION   DAOR01000253 DAOR01000000
VERSION     DAOR01000253.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 16950)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16950)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16950
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_2982"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    169..339
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5065 a         3421 c         3394 g         4899 t
ORIGIN      
        1 gctataaacc gtcaacaaga aggattggtt attgccaaca ttgatggcgc gataaaaagg
       61 attaatgaat caaagtatgt tgtaaagtcg cagaatgcta atggcgacta tgatgtttgc
      121 tcgaccgact taggctgggt ttgttcatgt cctgaccaca aatttcgtnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tttcgtggcg tcaaatgtaa
      361 acatattttc ggcgtagaga tttcatttgc gcttagaaaa gaagtagaag tcgctagaat
      421 cgcgcctcta acaaccacca cttgtattta ctgcgattcc tcgaatattg ttaaagacgg
      481 attgcgccat aacaaatatg gtgatattca aaaatacgat tgcagaaatt gcgaccatta
      541 tttcacaatt aattttggtt ttgaaaaaat gaaatcaagc cctcagacaa taacatctgc
      601 tctacaattg tatttcacgg gcgaatctct gcgtaatgtt cagaaattct tgaaattaca
      661 gggtctaaaa ataaatcaca atacaattta caaatggata aaaaaatacg tcacacttat
      721 ggcgcgatat ttggggcaaa tcaaacctaa tgtgtcagac gcgctttaca gagaatgatg
      781 tatcagtgca agtcacccaa aacatacggt acgattatag agatgatgct agaatagtcg
      841 tgcttgaagg gtattgtaat aattgcaatc ttggttatcc tcttgctgcg cctcttatcg
      901 agtatattca gagaaccaat ttacggcaga atgaccctat tgcgaatgaa tgtcctcaaa
      961 tgataaaatg caacataagg aatgaatata aaattacttc gccgagtaag ataacagtgg
     1021 tcaaaaacgt aattaaccat gatcgtggca caaagaagcc atcagatttt acgatattta
     1081 taacgggaaa cagcccttct ccaaacaact tccatggttc cacctcaggc acgtcaatat
     1141 caatacagcc tggtacctac aaagttatcg aagatgcaat ttctggttat gcaacgactt
     1201 attcctctga ctgttctggc agtatcacaa caggtaaaac ggtcaactgt gtcgtaacta
     1261 atgacgatat tccaccacca cctttcgtca acacccaaaa tgtgaaactc gggggatcaa
     1321 ccttcttaca gagtgctttg attccgttag cagatgtagg accttcatcc aacatcattg
     1381 gtggacatgt gtcgattagt tcacctacgg gaagcagtag cgatcatcta aagttggtag
     1441 ctgcagaaat cactgatgcc ggagtgcagc atgcagtcac tgtaaacttt acaaagacat
     1501 tggatataac aacaacagga caatcgctat atcatgttga tatgcgaaca tccttagctg
     1561 gaatcaatcc atttaccggg gcagcagata gagtaactca tttcacagct cttctacttt
     1621 ggaacaatgg gacttcgaac atcgtgttca acggtgacga ctacgtaaca attgcaataa
     1681 tttatgctgg aagatagagg gcgagaggaa tgtattcaga gacattgcac tgatgagaag
     1741 catagatgcc aattgtcttt gagattgtat caaagaaaac ttcgaaccgg tatcaaaaac
     1801 actactggtg ctacttctgg ccgcatatgc gccgacaagc atccaacccg cgataatgga
     1861 ctcaatggtt gatagcatgt aaattgacag catccaacac tcgataatcc tgccgacaag
     1921 tttttagcca taacagcttg ctttggtagc gcataaattt tcctaggttt gttgaactat
     1981 gctcttacta aaaatagata gtataattgt taattaattt tggcatacag aattatcttc
     2041 ttcagatatt ttatgagtat cagtagagga gcgcagatat atcataactg agggaaattg
     2101 gagtaccatg aactccttag attggcatga aataacaaaa gctaaaaaga tggtaagagc
     2161 tagtgatgca agcctgtgcg ggaacgcaat tgcagaatac cgtgataaca tagtcataat
     2221 agaacacggt gcaaagtttc gcgagtatat aatcccgaaa tctaaagtcg aacgatatga
     2281 cggaagggat gtgtatctga atataccacg cagcatgctc tcagcttttg acttctgaac
     2341 aatctttcat ataacgctcc agtctagagc ttaacgaaaa ttctcatttt cacttcgtct
     2401 tgtacagaat tatttataga gaacaatttc aatgaaacac gtagtactag catctcaagc
     2461 gaaactgttc cgtaacaatt aggtacaaaa ttagatttgg actattatat taggaacgac
     2521 cgttccatac cgaggcgttc ctccattccc ctaatttaga tgaacccttc aatatatcgc
     2581 agaatgatgc aatctacatt cccttcgtct tcttttgaac gtaagaagag caacggagga
     2641 acaaataggg gaaaaattca gattacagct gatattcttt atctgtccac agtcgctatc
     2701 aaaaagaccc atattatgta caaagcaaac ctgagctatg agcaagtcaa ctactatctg
     2761 actgatctgc tgaataacga ccttctctca caacatattc ttgaggatgg cgttgtattt
     2821 aggactagcg agaaaggcag ggaatattta aaacactata cccgactcat ggacttggta
     2881 aaggagagag aaaaggaaaa tcaaaaacaa gtacttgcac atatcgaagg aagcaaggag
     2941 gagaactaga tctttcaata ttaacacagc aggttcaact gtgaaaattc tcctttgttt
     3001 tttctacaat aattgagatc gcgagtttcc tacaaattga ttggattacg tttcattgat
     3061 gactcttcgt ctaggttcaa atattagcga cgaacgaaac gataaacgga tttagaaaca
     3121 ataaatttac tgggatcgaa ttattttgaa taagaagggt ttagataagc agatcgctca
     3181 actatcgagt ctgtctgcaa accacaacat atcattgatt ttatcaccac gttaatttat
     3241 tttttatttt tgaatccttt accaatatga ataatagtga ttatgaacct tagtatatcc
     3301 tccctaggtg cgcggtgagc atttcctact aacttcaatt ggttcgttaa taatttgttt
     3361 ttcacaatgc gtattctctt cattatcgac taatttatcc gacggtaggt caaaatatag
     3421 gacaattcaa gggaggcatg ctcgaatcaa tattaaatag tgcaaagaat caatgttaga
     3481 gtaataatga ttatacaaca atgtcatagt tgtgttgaga aaggaaaaaa cgcttattgc
     3541 aataaatgtg taaagtcagg tgattattat gatgtctaca aatacgctga ggataaggaa
     3601 agccatttca ggaaaggcaa gatgataggt acgataaagg catacacatg gcatgatgcg
     3661 atagaacata ttaaagataa ctttttccac aattgcagtg aaaatctcaa tataaattgt
     3721 gaaaaggact ttgcttatct agaagaaaaa cccgaatcta ttgttagaga tgaccgcgac
     3781 aacgcagccg cggaaaagcc gcacggatat acaatttatt tgaataacga aggaaatgaa
     3841 tcaggatctt tgtcatctct caaagaaaat ttttgggatt tgactgcggc tgttagcaat
     3901 aataattatg ataaggtcag tctgtcgcat agtgctcaca tggaacaaca gtagacaaca
     3961 gctgttgttg tagaagccaa aggaataccc ctacaacgaa agctagatgg aaaaagactc
     4021 ttcaaaatag atatcttagg tagtctgtcg tacactttat ttattatcag aagagagaaa
     4081 cttcttcata tttagtctca gaaaacaact ccaccagtgc ataatttatt taatacaact
     4141 tgatatcaat agcagacgaa tcatctgccc ttgaaaaata ttaaagcaga tctgaccgag
     4201 cctgaactca atgctctttg tttcagttac cggaataatt gaaagtgcca cctaatatca
     4261 aaattcctcc tacgtggctg catacaagag gaatacaagg atccacaatc accaacaaac
     4321 gctggtgtgc gaacctaatc ttgagaggcg ttggtagaag tagaatttgc tgtttgataa
     4381 tcaatccagc agctgttcac agaaaagggt atggcaattt gtgaacatga gactgaatgc
     4441 tattcccgtt tttcgaccta ttagaattat gcgcctagat ttcaagaagt gcaaactatc
     4501 tccatgcttc ttttctgatc gtgatcgctg cataaaataa tagccaactg aatacgaatg
     4561 ttgacatgaa tgtgaataaa ggccggtaca accagtatgg ataaccgggg tttcgtaacc
     4621 gggaatcgat cccgtagagc attccagtat acattagtcc cgagagatag aacaccactg
     4681 tccctatatc ggctgacaat agaagtacga ttattttaat aacaatgaat ggcctcataa
     4741 gtttgagacc caagactaga taatacagaa aagctgcgta aaatggcctt cgccagtata
     4801 ttccgccagt tgcgggaagg cttcgaatga agctcttcct ccaacggatt tgctgtcgta
     4861 ttagtcccac gagtgtatct ggcactccag ccgtcaccct tacgctcgga ctgtaaacta
     4921 atctccatgc atattttcgc cgatcctttt tcttgagctc ttcttgatcc gggtctgaag
     4981 aggatctcat tgcattcagg tcatcttttc ctgtctgtag tatggggata ttattattac
     5041 caatactgtt tttattgcta tcttgttctc ccgtattatt cctcctatcc tcccatatgt
     5101 cctcgggctt agcgcccaga acatacgctg tcagccgtct gtcggttgca aatttaaaat
     5161 tctcgatacc aaggaaatga tcatgagccc aggcgtgtat atattggtgc acagcctctc
     5221 ttctataaat cgacaatgcg ccagaacagc acgtaagaga cgagaatgat gtttccattc
     5281 cctttaacat tctaaattgt ccgtcgaacc atacatcttc aatcttgagc aaagtgtttc
     5341 ctttggccgc acctctcact cgcgcatgac ctgtcacagc gccgattctg cgatcagtat
     5401 agaatatttt tacggcgttg tcgacggcat cgctagcaag atcgcaatca ctgtccatca
     5461 tgacataaat ttcaccttta gcgatctggg atccggcttc aatagcaacc tttttaccta
     5521 cgctttttga gagatgaatt atttgaagtt tgggccctgg accaacctct tttctcatct
     5581 catccaagat tgcagtcgtc ccatcagtac ttccatcatt aacaatgatg acttccttat
     5641 tagtataaga cgaattaaga caagattgaa cacagtttct gatgttacct tcttcatttt
     5701 ttaccggaac tataattgag acgagaggtt ttcgatcttc ggggatgagg acatccttga
     5761 ccctaataaa gggatcgcgg tatttcaccc aggacagaaa aaatatatta aacaagacaa
     5821 atgaggtgag gaagctgtag aaaccgactg taaaatctac tacataaatt aataaataga
     5881 gttttattcc gaggataacg gcaatgctag caagtaccaa ataccgtact aaccaacgct
     5941 tcttgtccat ttccctgtat ttttccgtcg ccaccggttg tttgttctga acagccatga
     6001 aatgagtcca cctcaactat gtttctattg agatcattac tctgacaact tggtgatcct
     6061 ctacgttttt gcttgacatt tgctcgattc ctaaactctg atgtatattt ttcaagatgt
     6121 atgtataagt atttgccaac tttgtcctat tcaatatctc cttaagcgat gttcataatc
     6181 actcagaata agcatcattt agggcatttg acgtgtccag gctccatacc ttcttctctt
     6241 tcttgtagat gttctgagaa aaaccaagat atctagacct aacccttatc gtatgcgtgg
     6301 agtatatatt atgtggatca actgttacgc aagtatatac ccaaaaaatg ccgtaagtct
     6361 gtatatgcat gaaagcctag acgttggagt gtgtcggcga cgattcacga gtgtcgctag
     6421 aaaaggtgtt ttttgtttcg tatcttactt tccattcttt gatttctttt taacggatcg
     6481 caagagcgct acgttagaaa caaagatacg tattatattg aatggcgcgc cgtctcatgc
     6541 tgaacgagta aaaattatga gcgtccagag agacacagca tgataacctt tgcactgttt
     6601 ttaatcccgc cttccatcgg caccttcttc acgaagtacg gactcgtagg tcttttcttc
     6661 aatggtatgc tctcctcatt tattcctata ccaaccgaac tcacgacggc agccttactc
     6721 gccgcaagag aaaacgagat gagaatattt atcgccttga ccgtcggatc aataattgga
     6781 ggatttatag cttactacct aggctatact gggtacaatt tatcccatcg gataagaaag
     6841 ataacaaggc ttgacgacag gagaagccaa aggagttacg ctctgttagc gaaatacgga
     6901 tggttgttga tcttcatatc gccttggatc cccgtaggcg gggacgtgat tccgataata
     6961 gcagggataa aaaagtacga tttaaccaaa ttcaccgttg cgatggttct tggcaaaacg
     7021 ataaaggctg tggctatagt ttattttctt agtttaatta tggtgacatt ttttcattag
     7081 attattatcc aagagtaaga aacgatacct cttactccat cataatttct cctcgcgcac
     7141 taccatttac gaaaaacaac aattgagacg taatcgctgc cttaggagaa atacgctatg
     7201 gcggtattgg agcggatgga gattttagat atggaagcgg tgaaggacgc tgttggagaa
     7261 ccgcttgcaa cttgagcgtt tgcccatccc gatagacgct taacaatatt ttgtctccaa
     7321 ctgacttgtg ttcctctacg taagaaatca agtcctccat ccttttcact tggaggctgt
     7381 ccacagcagc tattatgtcc ccaccatgct tgttgccata ttgatcgatc gtgctaccat
     7441 gcaagcctgc cttgtctaca gcacctcctt ttacgattga atctactaat ataccttgaa
     7501 agtttgggtt taacccacta acacttcgag ccaagtccga agtcagggta gcggcagtaa
     7561 gtccgaggta tggatgagtg tagttacccc tctcaattaa aataggaacg atgcgttttg
     7621 cagtattaga ggatatggct aaacctatcc cggagaagcc gcccttatca gaaataacgg
     7681 ctgcggtatt tatgccaata acttgtcctt gcatattcaa caaaggccca ccagaattac
     7741 cgggatcaat caaagcgtca gtctgaatca catctgggat agagaaccct gaggtagcgt
     7801 caggcaacaa cctgcctttc tgacttacta ttcctgttgt catcgtgtcg tcaagtccaa
     7861 atggatttcc gatagctatc acttgatcgc caacttctag tgctgaggag ttgcccaact
     7921 ggagcggagt tacaggtttt gtaaggttcc cagtaatttt cagaactgcc aagtcattgt
     7981 agatatcagt tccgacaaca tttgcagtgt acctatttcc gtcaatgaaa gtcacatcca
     8041 caattttagc agtacctacc acatggttat tggttataat atggcccttt gtgtcagaga
     8101 caatccctga gcctagtgca gtggcatttc tcgtatgtgg attttgcaaa ttcagcgtcg
     8161 ggatctttct agtcacctga acgacagaat tctctacgct cttaaagaga agattaagcg
     8221 aatgatcctg agtttttaac atcgctaatt tctgctgttg gctttgtgct ctagctggat
     8281 attgaaacgt cgctaatgac gataaaacga ttaaggaagc taaagccaca attgcaaata
     8341 ttaagaacat tttttttctt tgcatctctc tagtaaggac aaggtacatc ttatagcagt
     8401 tactatcccc tcctactttt ttaaatctaa tctatcagcg tgtatcgaca agtatagtag
     8461 gttgcgtttt catagtattt gttttcataa taatcgttat tcatgattta tttaagaaat
     8521 catcatctaa tcaatgttgt agacttgagc aatttttcaa tccctaagcg atctaacctt
     8581 gctgccctac gcaatacatc tccttctaat ttttgtttgt atctctcaac cttggctgca
     8641 attgctgggt tctttagtcc tagtatttga cacgcgaaaa taccggcatt tcttgcaccg
     8701 ttgactgcca aggtagcgac tggcacgccc ggcggcattt gcactataga caaaagtgaa
     8761 tctaacccgt gcagacgtga cgacgtaccc attattggaa caccaattac tggcagcgga
     8821 gtgatagctg ccaccatccc ggggagatgg gcagcccctc ctgcaccagc tataataact
     8881 tgaattcccc tcgtctttgc tgtcttcgca tagtaaatca ttcttttaac ggttctgtgg
     8941 gctgaaacaa tttttacttc atttgtaacc ccgaacgaat tcaaaatctc ggccgcttcc
     9001 ttcatcaccc acaaatccga gtcgctaccc attattattc cgactagaat tcccgctaat
     9061 ctaaatcacc tctctttctc gtttcacttt tatcactttc ttagccatat ttatccttag
     9121 taatgtctct tcaaccgaat ctcccatcaa agtaatgtgt ccaagtttcc ttcttggctt
     9181 cgatattttc tttccataga tatggagttt taatcgagga atagaaaaca attttcttaa
     9241 accactaatt gcatatggtc cgatgtaatt ctccggaccg agcacattga gcatagccac
     9301 tgcggggcac aacaatcgag gctctgataa cgggagatca agtatagctc ttaggtgttg
     9361 ctcaaactga gaaatggaac atgcttcaat ggaataatgg cctgaattgt gtggtctcgg
     9421 tgctatctcg tttatcatta ctctatcgtc atcggtcaca aacatctcaa ttccgaagat
     9481 gccagctcct ttgagagcag caagtgtttg ttccgccatt tttctcgctt tacttgcgac
     9541 tttgtcagag acacgggccg gtacgatagt caagttcaag atatgatctt tgtgtatatt
     9601 ttctacaaca ggaaacgaag tgatttcacc gcttgcgttt ctagcgacca ttattgatat
     9661 ctcctttcta aatttgacaa acttttctaa cattagtctt tcttccccaa tgaaatgaga
     9721 aaacgctttc gagatatcac gtttagatct tatcgaataa ttacctctgc cgtcatacga
     9781 atcctgacaa gccttcaaaa taactgggaa tccataatct tcacacagat caaacaactg
     9841 tttctccgaa ttgaccagtg cgaatgtggg gacttttaaa ttgttttctt ttaagaacga
     9901 tttttgttta tacttatctt ggattattct cagcgtctca ggtgatggat taacaaaatt
     9961 aggatttttt aatgcaaggt ctatcagagc ttcagaattc gcaagctcta tttcgtatgt
    10021 tatcacgtct gatttgctag ccaagcttct aatggctcgc tcgtctttaa aatcggcgat
    10081 aattagttcg tctgagacag tagatgcagg acagcatcta gagggatcca gaatgcagac
    10141 cttcaaagcc atgcgtttgg cttccagagc aatcatcttt ccaagttgtc ctccccctat
    10201 aattcctata gacagaggtt ttgttatcga tagttcgttt agtctgccag acttcaaatc
    10261 gcaggacgta tactcgtgaa aggaaattaa agtcttcttg taccaagcgg tctaccacta
    10321 caaaaaattg tcgtccacgg tgggttgacc aataacatcc caagcgagtg aaaaataacc
    10381 atcataagcc caggtaaata gaggcaatag aaaaagtcaa aatagagccg aaactcagta
    10441 gaacgatgat tgtagttgaa aaaccaacaa agacaacaat tctacaagga gttctgcgat
    10501 aaggtcgtgt ctattgatcc agcaatcaga tttgccggaa tcgtcgatac cgacggaaaa
    10561 ttgttgggaa tttcggagcg taagggtcta actcccttgt taactccaga acagagagca
    10621 caatatgcga ttactgccgc cacgaggcag tttactagac taagatggga ataccatttg
    10681 ggtaaaatcc attacgcaag ctcacactat gcgaaactta tccgggcgac gattccgatt
    10741 accgacacag aaagccagct ctggtacgtt ctactcttga cgttcgacgt ggaaacagat
    10801 aactttcacg aaataataac aaagggcata atcccgatgg tatcgcaaaa cacaagtcag
    10861 cttatcaaaa aatagtcgta agagtaccgg acttagaatt ttctaatctt tccgtatctg
    10921 tctcccttgg ttattgtttt gcagacattc cgccatcaac agttaaaaca gtaccagtta
    10981 tccatttcgc atcgtctgaa catagatatg agaccgcacc ggaaatatct tctggatcgc
    11041 caatccttcg taacggaaat gacgactcga gcgttttttt ggcttgcatg tcctgtaagt
    11101 acggttctat catagggctg cgaatagtag acggggcaac gcagttacat cgtattccat
    11161 attctccgta ttccactgca atagttcgtg taaacattat tacgcctgcc ttggttactc
    11221 catagacgga gaacggtaca ttcaatatcg atcttatacc ctgcagcgaa gatatgttga
    11281 caatgctccc acgtctcttc tccatcataa taggaagaac tgcttttgta gttctgaatg
    11341 tcccgataag atttgtgtta attaatgacg tccattgctc atcagtcatt ttgtgaaatg
    11401 gggtcgggtc attaaggatt ccagcgttgt ttaccagtat atcaaccgta tcaaacgctg
    11461 aaacagtttc ctctataaca ttgataacat cagcttcctt cgttatatca gccttgactg
    11521 ctaaaaggtt cctcttccca ttgagatcag atataacctt cgttagcctc gagcgatctc
    11581 tgccaatcaa cacgacctta gcaccctcta agaagagtct cttagtcgtg gcctttccaa
    11641 cgccagtccc tgccccgctt actatcgcta ctttgccttt cagactcatt gattgttaca
    11701 gccggtgggc tctttttaag tacttttcgg aagaaactga agttagtgaa gatcttgaaa
    11761 ggaagctatc aaatcaagtc tgtttgtgtc tgtgtcctag tctgtgtaat cgtgcagatc
    11821 acttgtgcaa caatgcgtaa aaatacttta agctccgcaa catatagata acaaattgga
    11881 taataaaaag ttggttctag atactagcgt aataattgac ggcgaagtta ccaaaatgat
    11941 agagacagga gatatctcgg gcgctagtca gattattatc cctttggcag tccttgacga
    12001 attacaatcc caggcttcca gtaacctaga acatggtttc gtgggattga gtgagatcaa
    12061 aaagatacga gagcaatgtt cgaaaaagaa aatagatctt ttgttcgtcg gtgccagacc
    12121 tactcttgat gacattcgtc tagcaaaaca cggacgaatc gatgctttaa ttaaagatgt
    12181 tgcgatgcgg gagggcgcaa cacttgttac tgctgactat gtccaggcgc tagtagcaga
    12241 agctcagggg gtccaatcca tacatgtatc gactcccgca aaaactagca atctagagtt
    12301 tgaaaaattt tttgatgaga ataccatgag cgtccacttg aaggaaggtg tacttcctat
    12361 gggaaaaaaa ggcaagcctg ggaattccca actggtaaag ttagacgaag aaaggtgttc
    12421 tcatagtcaa ctcacacaca tcgttgagga gatatctgag gcatcgagag ttgaaggagt
    12481 gggctcggtc gaaatcagcc ggagtggtgc aactgtaatt caatacggca agtttcgaat
    12541 agcaataacg aggccacctt tttctgacgg gcttgaaata acaattgtca aacccatcgt
    12601 caaactcgct ctagctgatt ataatgctaa tgaaaagttg atggatcgat tatctggaaa
    12661 agcagaaggc attatcattt ctggtcctcc tggttccgga aagagcactc ttgctagcag
    12721 cctcgccgag ttctatttag aaaaagggaa gatagtaaaa actttcgaat caccgagaga
    12781 cttgcaggtt ccagcagaag ttacccagta tggtcctttg gaaggaagtt ttgacaaagc
    12841 agttgatatc ttgttgcttg tgcgtccgga ttatacaatt tttgacgaag taaggcgtgc
    12901 gcctgatttt gaagtatttt ccgatatgag gcttgctggg gttgggatgg taggtgttgt
    12961 ccatgcaagc agtccactgg atgctattca aaggtttatc ggtaaggtag agttaggaat
    13021 gattccacac attatagata caattatctt cgtcaagagc ggaagtatcc aaaaagtcta
    13081 tgacgtttcc cttaccgtaa aggtgcctac aggtatgcaa gaggctgacc tagcaagacc
    13141 gctagttgaa gtgcgagatt tcaacaccgg agaaattcaa tatgaaatct acacatttgg
    13201 agaagaaaat gtcgtggtcc ccatagcgga attaaataaa aaaatcaaac ccgatgcgac
    13261 tatacggaag cttgccgaat ccaaaattcg agatgttata agaagatttg attccgatgc
    13321 tgaaataaat attctgtctg aaaacagagt acagatcaaa gttaccaaag aggtggttcc
    13381 gctgattatt ggaagaggcg gatctaccgt atctgagctc gaaaaaatgt taggaataaa
    13441 aatcgatgtc gaggtgaaaa ctccagcact tggcaatgaa attaatttta acgttagcga
    13501 agccggttcc tcggtaacga tcctaattaa tcaagaagaa attgggaaaa atgttaatat
    13561 ctacatagat gatgaattcg tatttaccag ccaggttgga aagaaatcca ggataaaaat
    13621 tgacaaacgg tccgagaacg gcagaaagat aataaacgcg attttagctg gccgtaagat
    13681 caggattttt gatagcagag ggtgagaata gaatgattcg tatgttcagg gccgtgagcg
    13741 gttgtacggt aaacaataca aatactagac cccacatcag gggctcattt tgttatagtt
    13801 tcaaacgagt ggacaatctg ttcaatcgtt gagaggtaat tagcgtattg tgctctagac
    13861 tcagtatagg agaacgtgta tagccaatta ctctttatca tcaaaatatc cattgtttgg
    13921 aactggagct gcaatgaagg atcggaactc ttaaaagtat aaattaattg ttgtgcagga
    13981 atacttccaa cttcagtgct gtttgattgc aaaagattga aatccatcat tgattgtctg
    14041 gctttatcga gaattgtttt tgataatccg tctagtgtaa gtttgcttgt ggtctttgac
    14101 acacctacca aaaatgaagg tggaaatatt tcaagttgac tttgcaaagg tgagatgaat
    14161 tcgatcctag aattgttcgc agagtagtgt actattttct tccaattttc agggtattga
    14221 aattttatcc ctaagactgc atcttgatag ttcacgaagc tgctagtatt ttgaaaacgg
    14281 cctatcgaca tattcgcctt aaccgcagat aataagtttg ctggagagat aacaattgac
    14341 agagtgatag tggtaagaaa aattgctagt ggggatacta tttgcatggc ctatcgtctc
    14401 ccgagataga tattttaagt tatagtctta attctaactt gaatggctga agtataggaa
    14461 ttaaggatcg agatgattac gagagtatga agccatcaac gtctcttatt gtctctattt
    14521 ttcatttttc ttctggttta tgcgctttgc tctgtttctt ctactatgaa tcttcagaag
    14581 tcatagtgac aagtgcatga cctcccttga tatgagattg gtaatgaagt ctagtgagct
    14641 cggcaatctt cgagtccgta aaatatttga gattatatct tgccaaaact acactgcaat
    14701 cccgacaata cactaccttg aaggccaccg tggtcacgag ttaaaactaa aattcttatt
    14761 cttaaacctt taaatttttt caagcgtctt tgattgctat aattcctcag aagttgttgt
    14821 caaggatttg ggatgcattt tattattcgc gaaccgcact tttgagacct tataataagt
    14881 tatttctccc cttctctcta taacggctac aaccgcattc ttgcccattt ttgttatttg
    14941 gtctagtgct tctgaaaaat ccgtagcttc gacctgaata ccctcattta ttccaaagac
    15001 aacgtacttt gctggctttt tttcaaactc tcccctttca tataccctga agtctactcc
    15061 aaacccaaac ccttcttttg caacgtaacc tctgcttcga agatcacgat aaattagaaa
    15121 ttttgttaat agctctctat catgccgtag tgtaatgtta aagaatgact cgaaatcaat
    15181 gcacttaacc ttctttgtgt tcaatactag acgatttgtg tggatcagat acaatgcttc
    15241 gtacgtttgc aaaatatact ctgaatcttg cttctctccg aatcctttat ttcttaattg
    15301 atcttggaat ttgatatctt tcacgagaat cttcaagttt ttttcctcta agacagcttc
    15361 aacaacataa gggaactcga tgtcgagtgc actatcatgt gaaggcataa cgaataaatc
    15421 agagcacggt accaaattta agtattaaaa tctgtgagca ttattatgaa gggagttaat
    15481 taccgagttc ctcgaggcat gccatatgta aggcgagaat atattgcagg taagccgcaa
    15541 cttaagattg ccaggttttc atcaggtcga cccagaagcg attatgatta caaacttgaa
    15601 ttgattgtca ccgagaatat ccagataagg cataattcat tagaagccgc taggcttgcc
    15661 gcaaacaaaa ccatggctac agcaggggat acgagttttt tttcggtgct tagagtctat
    15721 ccacatgcta ttcttcgcga aaataagatg atcgcaactg caggagctga tagattgcaa
    15781 gaagggatga gaagagcttt tgggaaatca acaggactgg ccgcaagggt tcgcccggga
    15841 caggtaatat atgaagcaca tgtcactgca caaaatttaa accttgccaa acaggggttc
    15901 aaggtagcgt ccagtaaaat cggatgtcct gctgttacta aaatcacctt gttgaataaa
    15961 caattggtaa aagaagaaga tgactaatca tcggccacat ttcagacagc agctttcgag
    16021 ataggatctt cgccgagatc tttgtgcagt gatgggaagg aataaaagct tgtaggttta
    16081 tgatacatcc agaaaaagtt tacaatgttc aacaagattg gtgcggtcat acttttggtg
    16141 ggggatttaa aagaatctat tgagttctac cgagacgttc ttggaatgca actcaaacga
    16201 gaatcagaag attgggtcga attttctaaa ggcagtagta ccgtgcttgc tcttcacccc
    16261 gcaaggaaaa gtcgtagcaa gaaaacgcag aaacgtgctg gtatgttagt agggtttaat
    16321 gtaagtgatc tacaagacgt ctgtggcgaa ttggaaaata aaaaggtaaa attttataag
    16381 aaacaaaccg aagaagcctt cggaaagcac gctattatag aggaccctga cggtcatcta
    16441 atatcacttg tcgaactaag ctcaaaagat gagttcagtc aaatacccta ctatcacggc
    16501 tttgctccag aatagcggta aacccaactc cattattttt tagttattaa ttatttttta
    16561 gtagttggtt gcgtttctag aaggattttc cttgactaaa aggattttcc ttcgaattat
    16621 caagccatag gaatacatat tcatgctgac tggcgctatt atcgggcggc caggtagact
    16681 tttcgaggcg cctacggatg tgaagacagc atattcaggt gaggagcatg tgccgattgg
    16741 tgtcaatggc acaatggtga cagtcagctg ggacttcttt gttcggacag agcctccgtc
    16801 taggcgtgcc gtgtgcagat tttcgatggc ataacattga aaatgacgtg cctgcgattg
    16861 aggaaatgcg caggaaatgg taatacagag aaagcctgcc tggatcaaac agataaatct
    16921 tatcctgctc gcgagcagga gtgcatctgg
//
LOCUS       DAOR01000254           12467 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_58887, whole genome shotgun sequence.
ACCESSION   DAOR01000254 DAOR01000000
VERSION     DAOR01000254.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12467)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_58887"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3952 a         2341 c         2179 g         3995 t
ORIGIN      
        1 tctatgagta actattcttt taaacggtca gtggctgatg ataatacaat aactaacttt
       61 gtaactcatg tatttgttct gaattttaat gtaactccac ctattgcatt acaacgaaat
      121 gtttattggc atcgattcta ctttaccatt catagcaatc cctaatgccc gattactatt
      181 ctgcggcggg aattagtacc attcagcatc tcgaccggtg ttggcactaa aatactataa
      241 caataaaaac gtatgttgtg catgtttaga cgattaaaaa tgtccgctaa taataacaca
      301 agtaaaagag aaaacattga acaaattcct aaggaggaga atataggaga agtacagaag
      361 gcggtcagtg agcagggtgg ggaggagcag gtttcagtct ttttccttca caagttatat
      421 aacaaacaaa caatctatta ttctctataa ctttagagag aataatcttt tatgttttta
      481 aactagagac tatataatta catagattga actcttcaaa ccgagtacgt gcccgtggac
      541 ataaagtatc aagggaaata acacctgaac aagcagcgga caaggtgaag gacgtagcgt
      601 agcaaggact attcgtgagg tatcttctac ggcaagagac acagtaaaat aattccgtga
      661 gagtggagct attacagaac tcgcagaggc agtacatgaa gctgctgcaa caagagatac
      721 ggcaaaagaa attagtgata cagctaaaga ggtcagagat agccatctag cagcaaacac
      781 agctagtgcg attgaagaag cagcaaaggc agcaggtgaa acattacgtg cagcagaaga
      841 gacagccaga caaattccca aggtagcacc caagacaagc aaaaccgtaa cgacaggagc
      901 aggcaagcca cggaaagcaa cgaaagccac ttcggacaaa acagaaaaga caatcaaaaa
      961 agaagctgca aacgcacgca ttaaagccac taaccttaag agaaaagcaa cgacgcatct
     1021 ggctaggcaa aaacggtcaa agcatcaagc gggaaaaaag tcagctagaa agggaagcta
     1081 gtagcattat tttgttaatc ctcctctatt cccttttttc caactgatat tatttagtat
     1141 tagcccagcg ttatcattta atgcagatcg cattaaatta caatagttaa ttaattacaa
     1201 cagtacatat aattatagag cttatttgta atgccattaa atttttacca atcatattgc
     1261 tcaataattc tcaacggcct tgacaattat tttcgctgca ataaacttta tggcaggtaa
     1321 tttagtttga ggagtattcc tatctatgtt agcgatatgt ggtacattct tactggtaac
     1381 gaatcatgaa agacaagcag caaaaagaag gtctgtaaat tcacgaatgc tagctgatac
     1441 gatgagatga agatgaaata accaaaccct gctaagactc gcactaaaaa tttttacgtt
     1501 ctatcaactt cgccgtctct tgttcggtat tctttaaggt catgccacca catcatagtt
     1561 tatctaggag tatatccata acatgaccaa aggtacgagc atcatggcca aaaacaactg
     1621 gtgctaaaac aacatttctg ggcgtgttat tacacacaaa aaaaagacta ctaacttacc
     1681 cactgaatca gatactacct aggttccctt gtcagtaaca tggcactgat tttatctacg
     1741 atagtttcgg cgctaatcgg ttttgttgca aattcatcag ctccataatc caaaaccctc
     1801 gtcttatcat tttcatcttc agcaagtgcc ataatcctga tattcgaatt aatccttttt
     1861 actttgataa tcaatattgc agctctatca gccgctattt ttccgtctac gaagacgata
     1921 tccactttac catctaattc acttactttg ttcagacatt cctcagcgtt atatgtctta
     1981 tgaacttcat atcccttaag cccaaaagta cctgccaaaa tcgaggtaac gtctctgtta
     2041 tcatcagcaa taattattct gagtctagat ttgatattcg ttctccttct ttgtgataga
     2101 actatacggg ttactaaaac tttattgttt tctgattttt agttgctcta gctttattta
     2161 tctctgagtg atcacatggg ataaaaaaac tgaaattgtt acatagaatc aattgttaat
     2221 gaatgtgaga gttattaatg tcattatctt ctgaaggcaa actaaaaaca aatgtagctc
     2281 ctttcccatc ggagttattc tctccccata tcttcccacc atgagcttcc acaatatttt
     2341 ttgaaatgaa taaccccaaa cctgtgccta tctcagattc ggtagcgaac attgagaata
     2401 gtcttggtaa aatttccgaa cctataccga tgcccgtatc ttttacacta agaataactt
     2461 cgtcaatatg atttttacct ttcttttttt taactgaaat agaaactata ccttctttgg
     2521 taaatttgat agcattagtt agaagattat atatgacttg atttatcctg tttttgtctg
     2581 cagttacgaa tatttcctcc gcagtagtgt ctgctcctcc tgatactctt tcttcctctt
     2641 ctttcttaaa ctcgtataat atcttcgtat tcccatcact tttgtcaagc tgccttctat
     2701 gatcttctat agcatctaac acaatctcat tcagattgaa atgttctttc ttcaatttta
     2761 atgacttatc atctattctt gtaatgtcta aaatttcaga aacaagcctt tctaatctgt
     2821 tggtgttacg aactaatctt tcaaggtact catttttcag tgtaatttct ttttttatct
     2881 tcctcaaatt cgtattctat caattctagt gttcctaaga taggcaagat gggagatctc
     2941 atctcatgag tagcgatact gataaactcc ttctgtttat catatttagc gcttacttct
     3001 ttgttaactt ctttaagttg gttgattgat tcccttaaat gcatgacagt tgcgttaagt
     3061 tggtcaatcg attctgctga cggctcattc gttgttgcag tgtcgcttcg ctcatggcta
     3121 ttactggctt ttgtagacac taatatatca ccagcctatg tactgaattg gacaggaaat
     3181 gatataaaga ttcatagttg attctaagat aataaacgaa gatagaagcg agtagagttc
     3241 atatgccttc ttgtcttcat tcccggccaa tggaatagtg agaatgactt taagatctac
     3301 taaattgagc cctatatatc ttttatattc tctgtcagtc attgtgtggc tcatcagcgg
     3361 atcagcgttc tttgagagtc tttcagggcg ttcgaaagca catcgtcgaa tttaatatgc
     3421 ggcacaagaa aaggagatgg tagagcgaag ataattggtt tcatcaaatc ccatccagct
     3481 aatgctacgc caaatcgcat ccgaactaaa gctcgatttt aagactatca tccaccacgt
     3541 gaagctatta acagaaaatc gtttggttat cactgagaat aaagaatctc atgacatact
     3601 ttctgacacc attaatggaa cgaatagttg gtggagatac tgctaaaatt cggaaaaaat
     3661 aagttatgtc tgcctgaagg ttacgctcag ctttaatcct tatatgttat atatgataat
     3721 atagccctga cgatgaccgt atatcgtaac aacgataggt aagatgattt cttattttta
     3781 ttcacgccaa agatcgccga catattcaaa gaatatggtc tttaagaaca gcccacgtct
     3841 taaaatctct aaccgaatct caaggtagga tgatgtaaca ttgagaacta ggtttggtga
     3901 aaatacattt ggcaactgtg taacattaac tattgatggt caagaaacat ttactctgat
     3961 gtatgagaaa attttcaaag caaaaacgag tatcttaata gccaattatg agctggatcc
     4021 tcgcttgagg ttcataagag gagattcaca atcttcttcg ttcattaaca ataatttacc
     4081 gcggtcttca agcggcttca catcagaacc tgaaaatatt aagttaactc accagaatta
     4141 cactgcatct cccttgcaag atcttttatt tgaaaagaca aaacaaggag tagaagtcaa
     4201 aatcattgtt tggcaaccaa aattaataat tagactatta cctaccgcat ataaacgcgg
     4261 attgagtgga agagtaggag aacttgagcc tctagagaga cttgtacaac attctaaaat
     4321 tgcaaaaaat ttgatggtaa gggttgataa ttctgcccct acctttacat ccggtcatca
     4381 cgagaaattt attgtaatcg ataacaagat aggtttttgt ggaggtctag acttgtctca
     4441 cggtaaatgg gataccaata gtcatgattt tgataatcct ttaagagatc taaatgcaga
     4501 gccatggcat gacatacatg caatggttga aggtccagtt gtaacggatt taacttacca
     4561 ttttatgcag agatggacat attcaataac taaagatatc gaccaggcca agagaataaa
     4621 gatcgaacca tcatttgaaa atcaaaattc cactggagat acagaggtta ttgcgctacg
     4681 tacttggaag caactgaata ataaaggtga tgataatgat ggtagtaata tattatcgtg
     4741 gtatgctgca atgtttggaa aagcaaagta tagcatatac attgaaaacc aatttgcttt
     4801 tcagagtgaa cttataactc agcttcttgt caagagacta caagaagagc aaaatctaaa
     4861 agtaatagtc gtaagtccaa tggaaccaaa cctccctggg tttatcagtg gatttgtctc
     4921 aaaagagagt atcaatcatg ttaacagaaa tctggaacgt ttaagaaaag ctggagggag
     4981 tcgtgttaat acatattgct tgatatcaca acacaacgct gctaacgaaa aaaggaaaca
     5041 gatctatata catgcaaagg taatgatagt ggatgataaa tggataacta ttggctctgc
     5101 aaatatggat aacgatggat ttaaaaattc cactgaagtg gatcttggta tcacttcgcc
     5161 agtacttgcc caacaactta gagtcaagct atggcgtgaa catctgccag aagaagatga
     5221 ttcgtcaaac aacagtgttg atctaaacag ctttgacgaa ggctttaatg catggaaaac
     5281 attggcaagt gacaatggaa caagaatttt aagaggcgaa cgtatacgag ggcaaatata
     5341 ttactataac tttgaagaaa tgaaatgccc tccgccttat gctaatgcaa gaggcggcaa
     5401 taaatttaag ttgttttaat ttgctaaaag gtttgtcgta gatacaataa catacgttta
     5461 aaacggagta gcttctattg tttagtttga tattagatca actcggatac ggtatgcgat
     5521 acaaggacta agggatttct aataaccaat cttctctcat acatacatgc atgcaacaaa
     5581 tgtcagtgtc gtcgcttcat ccttgtcctt ttttcccaac agaccactca caaactcagt
     5641 cgtctggtaa tgattttttg cccccccaca tcatctgctg ttgttgatga tgatgtgaaa
     5701 tgcaggataa ccaatctgaa atcggcccac ggtatttagt aatagtccct cttacttcgt
     5761 ttttattcat ataatatact tggttctgat gatagattag ttgaataaaa ctgagattag
     5821 atatatgctg taacgaacag aaccatagta gtagccatgg cacatcccca tactatttta
     5881 ttccaatcaa agatcttctg tccgtctagt actccaaatg gtaacaagtt aaacattgct
     5941 atccatgcat tgaatgatgc acctgcatac aaatacaaat gatgtggata tatataagtc
     6001 gatattagaa atgaaaatcc cattaccaaa ttagtcaatg gtcccacgag tgcaactctt
     6061 ccaaacttat ttctgtcaga taatccaacc ataactgcac caggagctat aaacttaaat
     6121 ggcataatgg gtaaagctga aattgctgta actataagtc caaatagttg tgcacgaaac
     6181 tcagcccagc ttccataata ctgtgctaga aatttatgag ccaattcatg aacaagaaaa
     6241 gcactaatga atatagcaag aaattgccat gaaatatacc tgtaatcatt aaatgataaa
     6301 cctacagcgg ttattaaaag tgtagcaata aagaggtgta acagttcggt ttttgaactt
     6361 tttatcttta ttgctctaag taatgatctc ccaatgctat tactaaatga tgatgctgca
     6421 gggccaccat catagttgcc ttgcatcata agccttcttt tttctgcata tttatttata
     6481 aatggacacg agtgatttat gggaattcta tgaagatgac aaaacgattc tccgcagtaa
     6541 ttgcaccgaa atggaatgct ctcctcaaga ccgcacgtat tgcatctcat tataccgcct
     6601 cagctaaaat aaccacaatg gtaatattaa ctgacgcgtt taggggttgg tgctctatgg
     6661 ttaattcctt atctttgttt taattataga taataattga ctaaatctga atggcttgat
     6721 tagtacctta tcctctttaa ttgctctaat ttgttctact tcttctgcct cttttttaca
     6781 gcatcaaatg gtgaagtagt gacaaggatc attctctgtg tcagattaat cctcttgatc
     6841 tgtactagac cgacgcgatc tgaatcataa taagaatgat cccgtttggc ttgcataaat
     6901 tcaagaacgc tctatcggac tcgatagtat tcgtgaacga atttaagttg gtggttgata
     6961 ggtaggttat cggttgtcct cccgggaggg tacgccgtac cttgcataca aataacaaac
     7021 taaatggaag ttggttgctc caaggcaatg aacttgtaga aaactaagtc agcttaccta
     7081 acgtattcaa taaatgttcc ctttcatttc ggatgatata ctataatagg atctgtatct
     7141 agaaaatgta tatggaaatg ccaactattg atatctccaa gaacaataac tgatgtttac
     7201 gcaaagatgt tgactagctt tctagataat atgattaatg caacaaggct aacaataatg
     7261 tcgcgttcag aatcatgata ataacattaa taactccacc aaaatgaaca tgaccgatat
     7321 atatttagct tgcagatatc aatttagtca taataaatgg tagttcaaga gtgcattctt
     7381 tgaatcattt ggaaatatag tgaaccagca ttaccccaac gtataggtgt agcaagaatt
     7441 acaacatctg cccaaaggat aatgaaatca tcatcaacaa tatattctat accatattat
     7501 tatctgcaat atatccacgc agttaacaca aattttaaat ccagatagct taacagaacc
     7561 tttatactat gtgtatatac tgatgatagt aaaatgaaga aggactttat catttcaaag
     7621 atcgaagctt ctcaagatgg cgcaccttac atttatgtca catttagtga cacgaatgac
     7681 tacaaagcag gcggcgaaaa gcagcaaaat ccttttggtc caaacatgat ggcatttact
     7741 tccccggaag atttgatgaa aaatttaccg aagatgatgt caaatatttc acggatgacc
     7801 ggtggtggtg gcattcccac tgattcccct acatttaaaa tgagtatgaa agagtatgag
     7861 gacattggta taaaagtggg tgataaagtt acgatagaaa ttaaaaaatc agaaaatagt
     7921 ggaatttaaa catggcaaat ctaaagattg tcacagagtg caaacactaa ggtattcaca
     7981 ctctcttaac gcttctttcg atttgataat tcggtcctct ctagtagtac acgaggagat
     8041 atctatcact actttgccat tttgtggtgt attctcaatt tttttactgt ctcttagagc
     8101 ataggtatta ggtaggaatt gtgttttcat tttttgtcag gtaaaatatg gattgaccgt
     8161 acctctttct gcataatctt tgagcaaaaa caccaaatgt ccccaggttg tattacacct
     8221 actataataa tcgccagttg aaggccagcc agtatgacta aatctcaatg ttgtttgatt
     8281 accatcttta ggaattattt cccataatag tttggtgtcc ttccattcat cagcagccac
     8341 accacctatg catttccatc tgacggtatt gtctggaatt aattcgtcaa catacatttc
     8401 gaatgtgaaa gagtggttat aaaatccaaa atttagtacg gaaccttttt tagaatctcc
     8461 agaaacatct gttgtccacc aactcttcaa tccttgattt gatgaaactg ctgaataaac
     8521 cttatctgga gttgcatcta tatcaatttt atgtaaaatg tctgccattc tagtctatct
     8581 ccctttgtac ggcctcagtg gcacaaattc tatgccattc taggctatct ggtttattgg
     8641 tagaattcat agccattttt gcgagattgt ctagggatgt catgctgccg ccttcgtttt
     8701 taaactcatt cacaaacctg tgcacgacag ttttatgtgt atgtgttgac attatctgta
     8761 catagaagga cctatattta aagatttaaa taatgcttta agtattatat tattataata
     8821 agtcgtccgc tttactacag agaacggagg tttgttaaat gagtagcgaa acctctttga
     8881 tcgtagcatc accggaatta cgaacgcaac tactcgatga tcccgaactt gtaggaaacg
     8941 tagcacgacc gatgaatctt gagaagagat tctattgacc ttgttgcaga aaaaattata
     9001 aatcatgata atctgtcatg gaggctggaa gacataggta aggcaactta taaacaaaga
     9061 atgaactaga gttttatacc accataacga cctctttctt agtactattc ttttgagtgt
     9121 gatcctaaag tccgaaaaag aatatagagt tgcataaatt tgctctagtt atccattgtt
     9181 caggtcgcag acgatgctaa gaatggctgt cagttaaaaa tgccatattc ataacaacgc
     9241 gcacctcctc atatggatcc cagcaacatt tacgagaatc aaactctggg ttcacgtatg
     9301 tggttttgca gactatattc tgcaaactta atttgtccta gaatttacat aagaggtaga
     9361 acctaaacta cagattaaca cgacaagcaa gaatgactta accacaaaaa ccacagcctt
     9421 acagggcata ggggttgtac gtttgaaaat gtgttattaa agttttttag aggctaagac
     9481 tagcatgttc ttctcaaaac ttaatacctc aactcagacg attgatgctt ctatttgggg
     9541 ttttatggat aaaccggccg gcttcttctt tctattctgg tcaaaagatg ttactaaatg
     9601 taaagtcaac gtccaatctc atgaaatttt tccttccaga agtactataa gtttaccttc
     9661 ataggttcct gttatattta ttagttgtaa accgatcttg tctagttcgt ttatgaagtg
     9721 ctgattcatg aaccttgttt ttagcttgaa acgaccggtg tccatatctt ggtctagact
     9781 ttttatctca atgcctatca attctatttt tttataccag atatctatat cttcttgaga
     9841 tttatccatt gtactataat ctcttaaaaa gattgattat gcattaaaat gttcctcaac
     9901 tccacttaaa gttctcatag aagtatggaa cccaatttat catcaattat tatgtggaga
     9961 ataattaatt atcttaattg ttatattttg tgcgtcgacg attgatgata gctgtatttg
    10021 tacaatagat gagtaggtcc tatgctacat ggttttgcca gcattctacc acatcatgaa
    10081 taagcctatg aatgagtctt aatgcttgct cgtcatttct taattaagtg atgttgtata
    10141 cggatatgct cttctaacac ctggatatta tcggtatctg cacttcatat catagtggct
    10201 atcattgtat attgaagatt taatgcggac tagggaaagt tattagaaac atagtaaggt
    10261 gttgttttac atcaactgtc aaatgcctca gataatattg ctgaggctcc tgtttttctg
    10321 atgcaaataa cacacttttc acagtaggaa aggactcatt tttcacaaca ctccttgagt
    10381 tttgtgagtt tgcgctgagt ttgcttctta ctaatacgat acgcttgttg taaatcccaa
    10441 tagattatgt cctccttagt tttgttaaac ttctcaacta ccaagccttc aatctctcga
    10501 ctttattgtt gtcccgaccc cctatacaga atctccgttc tcaattgctt tagaaatgag
    10561 tctataaaat ctgtacgctg ttctgctatc tcttttgcta tcttagtatg catggaattt
    10621 tttaatatca acaatttctt tttaatatgg tctagtgtcc accgagtatc atctaatttt
    10681 ctatctgatt cacaaaatgg atcttttgaa tcataaagag acctgttttc gcttcctcca
    10741 acactaaaag ttctagctat tcctatcgca cctattgcat ccaacctatc cgcatcctgc
    10801 aaaattttac cctccaatgt ggaaggagtt aatttctttg aatagctatg tacacgtatt
    10861 acatcgacaa ccttttctat tttttctgct ggataccact tgtattcaga taatattttc
    10921 tttgcaatct cagcactctc atcggctgaa tttattgtct ttgtgcttcc ctttggataa
    10981 actaccaaat cgtgtaatag ggcagcagca agaacaatat ccaaatcagc accttcttct
    11041 ttcttgctta ttatttcggc gtttctatat actcttagta tatgttgaaa atcatgagcc
    11101 ggagattgat tatgtagcag taattcgact gttctcaata tttgttccaa attaactgtc
    11161 atatccattc ttgcagagag gatcaattct attttaagtg tataagtcgt attatacttt
    11221 ccatcataat acgaatattt cataatgcgg aacggaatgt aacatattca gtcgatagtt
    11281 attgccactt tacttattta ggactttata tagtgtcact aaaagcacta gatcagcata
    11341 ttgtagccat caagacctga ggcgttcttt aatgagttgc tatatcagtt tgcttctttg
    11401 ccgatactgc attctggcaa atctggactg ctatttttgc tgactttgca caggttaaga
    11461 ttagaaagga ggcatatacc ttcagcaata gttacaaaat tcttgttgag gttagtatat
    11521 cgcagactct tggttggtca tgcaaatttt aatatcagca acaacatcta agacttttaa
    11581 aaaatacaag gctgctggat tcatttataa caataccagt ggttagactg gcctaagagg
    11641 aaggtgtttt ttgcctctat cagataaaaa ataattaggc gttccgaatt agtcctctta
    11701 ttttctgttt catttctgga gttatgtctt ctgctttcat accatgaact ttcatagcgt
    11761 gctctccgcc atttctcata acttcttctt cattctcgcc tttgacaaca tgatcgcagt
    11821 taaaccctac ctctcgacaa cttaatgttt tcataacttg ttaaataata gactatctta
    11881 tatgtaattt gttataataa cgaacatcga ttatagacaa attttgattt cttggcctag
    11941 tggtttcagt gagacccttt tctacagcct gaatctcaaa aaaacttgat cggtcgttgt
    12001 tatgttagat gattgctgct gatgtgtgat gtctgtttct tgaaatcctt gaatgtatta
    12061 tcatctatgt aattcgaaaa gtgaactatt taatctatat acttgcagat agtaacatct
    12121 atttggaatg atctatctta acatgaccta gaagatcaat ggaaggcgta aataatacaa
    12181 caaaattctt ttctgatgcc tcatcatttg ttgcaaaaat gatatttttt acgaaaggta
    12241 aagggttaca caaagagtat cttacaagct ttgaacttgc cctgcgtgat gctgcaataa
    12301 gtgatctaaa tcttgtctct gtatctagta taatgccacc agaatgcaaa accgtaacta
    12361 gggaggaagg aagaaagcac ctactgccag gacagattgc ttttgcggtt atggctaagt
    12421 ctgctactaa tgaaccaaat aggttaattg ctgcgtccat tggttta
//
LOCUS       DAOR01000255            2998 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_92934, whole genome shotgun sequence.
ACCESSION   DAOR01000255 DAOR01000000
VERSION     DAOR01000255.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_92934"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          936 a          571 c          542 g          949 t
ORIGIN      
        1 gcttggtatt atttctgtta tgccgatttc gcatatctgg ctcacagtca agctgtgatt
       61 aggttcagtc agttagaaaa aataagcaag aactttcatg atgcttaaaa tgcaacaagg
      121 atcagaaatc ctaaccttaa tccattaaag gatgcaagaa ttactatctt actaatttct
      181 atattgtagt agatagtatt taaaatattt agcaaggatt tatgagttat ttgaatcaga
      241 ttggtgggat aagatgacgc gagaggcaaa gtacatcatg acatcaaaaa acagaccagc
      301 tactacagca tggtcgaggc agaagcctct cgcgtacata aaataaaata tcgtcatgtt
      361 caatcacaaa tttggacctg cttcatcctt atctttacat actttttcgt agaaatgatc
      421 catgaattcc gttagcattg agtgagtttc atgatctttt gttcgcatgt aatagtaaag
      481 ttcttcttta cctgcgtgat gatctacacc gacagattgt gcaagctgta aagaaagata
      541 tgtaatcaat agtttccgca ttactatgtc cttcccataa ttcacaaggt ctacatcgag
      601 ctcatataat ccgtctttca tttgtgagtt aatatatcga ttttttgaat cacggctttt
      661 gagtgagaac gatacaccca tcattctttg tttcatgtgg atcttttcta ccacatcgat
      721 aaagatttcg tttagattct gatcgtgaaa tagaatttct gagagctggt cagaggccgc
      781 agcctcactg atttccattc tattaaccaa tgtaaataat atgtggcatg ctacgcagcc
      841 gttttccttg actctagtgg gcgtcatgag tacagcgttt atttcttgct caattatctt
      901 cttggctaca tcatgtctgt catttgttac cataaaatca atcaatatta aagaacagac
      961 aggatatcta aatacctagt ttcagaaaca atacgtcaca tatttataat ctggagcggt
     1021 acaatgacat atctgtgact tgcctgatcg ggcaattccc tgtgtcctgt tctgggcctt
     1081 cataatagct taggagaatt aattactcct ctgcgtgcgg taggtacaaa aaatattctg
     1141 gctcatttaa atacaacctt tttcatatgc gaaccacatt tgcggcaggt tctttcactg
     1201 accttgctac cacaggctaa acaaacatag attggttttg tattgaagtc cgcactatca
     1261 ctgctactac tgccatagta acctcctctt tccacattaa acccttttct ttgaatgtaa
     1321 aggatcaccc ctataaacat gaacacattt agcataaagc ctattacgaa accaaggaaa
     1381 tacgtcgcca acatacttgc tcccataatg gcaaaagcaa acaataaccc acgtttaact
     1441 aatttactgt tctgcatgct tatccaacgt atatagcaag ttatagaata aaaatggtcg
     1501 catcatctgt gaaaccaatg taaaaagtct cggatgatga atgaccggcg gagacacctc
     1561 tgataacaac tgatcgaaac agattatcgg cacaggagca caggacttta atattatgac
     1621 ttttacaaga taccttgtta tttatcccat tttcctattt tacatcaata acgaatctca
     1681 aatttcgaaa tacaacagcg acctacggaa gtcaagttat acaggaacta cgatatacaa
     1741 tcggttcgta tcgatcgaca accggtgcgg aagatgaatc aagaatttcc agaaagtagc
     1801 tagaaccttt tccatctcat taatttgaaa attttggtat cctcattttt gacatcaatt
     1861 taagcagtaa ttgccttcga cctgaaagat ataagtagga ttatcatcac aactgttgca
     1921 ggaagtgtaa tacgtgtctc gcgtcattag taatgcaatc gcaagttgaa atcacgatat
     1981 cataagtcag cacttcgatc gaagactagt ccattgtttt cttgaatttt aagttcagta
     2041 gataaaacta agaaattatt cacggtaact gacctcctct gcctattggc acgtattctg
     2101 gcctttattt agccttgaag cggatataaa cataaaaaaa gtaatgattg tggagcagtc
     2161 agcttcttac tattttacta ttacctttcc taccatccaa ggatggacaa tgcataagta
     2221 gttgtacgtt cctgcttttt ggaatgtgac tgtaaatgtg tttgaagagc caggaaaatc
     2281 cttatcgtgt ccctttggaa gtataaaacc tgagttgacg tacttttcag ttccgcttag
     2341 ggtgtagttt gcgtttggat tcattgtctt tacattacct gatgaatcaa ttgctactgg
     2401 attatatgct cttgcgttca acgatattag agtattcatt ccgtttttac caggcagaat
     2461 gagtggttga ttattagaac taggaggcaa aggcatgaat tgtgttgaac ttgaaacacc
     2521 aaaaggtgct tgaagaatat cctgctgctg caagccctat tgctgctaca acaaagacga
     2581 aacctagggt tatcttagta ttcattattt agtcaataaa tattctggtt tataaggaat
     2641 tattgattaa tagaagtcgg taagttatat gctgcctcgt tttgcggaaa gagagctgat
     2701 gacgagtaac atatgaagaa taggtcaata agtggtagtt ttaatatgaa atttattaag
     2761 gaggatgtta cacaattcat tatgactcaa gaattccttc ttgatctcca aggtgacaac
     2821 tatgtctgaa tctcaagaaa ttagagaaat taagagagta tttgcggggc tacagacatt
     2881 ataccacact tcttttccaa aatcagatcc atctttgatt cacgatttgc tgaggcgttt
     2941 gcaaaaaaat cccgacattc caccgatata tatggttgaa gtgttcacaa aaccaggc
//
LOCUS       DAOR01000256            7696 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_26973, whole genome shotgun sequence.
ACCESSION   DAOR01000256 DAOR01000000
VERSION     DAOR01000256.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_26973"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2069..2244
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3897..3997
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2395 a         1364 c         1357 g         2303 t
ORIGIN      
        1 catgtcggta catcgttttg tcaaccatgt ctggaagtac tacagttaat gataacagtg
       61 gggacatata ggtagagaca ggttagtgat tatcaattta tgaactatga agagaaaagt
      121 actaggcaac accaagacaa agacaaagag agcatcccta caagaccact aaagctgcga
      181 ctgaagttat tggaaaagaa gtcgcatact ggaacagata taatgtcttt taggtttgct
      241 cgcagtgatg accagcaaca aaattattat ttgaactaca aatcagggca atatgctatt
      301 gttgatcttg gaacaaaaga ggacccagaa ggtccagtac gatcctttac aatcgcttcc
      361 cctccaacag aagaagattt catcctaatt agcacaagag gggatactcc attcaagaag
      421 aaacttgcaa gcctggatgt tgaatctcct gtgtagataa cagctcctct gggaaaattt
      481 gttttgcatg aagattattc aaaacctgct gtatttttat ctggaggcat aggtgttacc
      541 ccatttagaa gcatagtaaa aaacgctaca gataatcaac tccctgtaaa gacaattgta
      601 tttgattcta accgagatct agataacata ctttacaaaa aagaattcga tgaatgggca
      661 gatataaata aaaatttgaa agttatttat acactagatg taggtgataa tgattggaaa
      721 ggagaacgag gtttcataaa caaagcgatg gtaactaaat atttgcgcgg gtccatccaa
      781 gaagatgtgt tgtaagagat tttaaagtct ctgataatat ctggaatagc cggtaaaagc
      841 atagtttctc caaacatagt gaccaagagt gcagaaccca atactatgag agttatccat
      901 gcagaaccgg ggatcttcac ggatgtagta gatgttcttt tacccttgtt atcctctgga
      961 gacgaaggtg atattggtgt ctctgccaaa tatatcagat ccaagtcctt gaagatttct
     1021 ttgattttaa ttttggggct gtttcatctt caatttcaac ttcataacat attttgtcat
     1081 tctttttaat aattgtagag atatgaattc atatattaaa aagctcccgc caacttgaaa
     1141 caacattatc atatactacg agacgcgaaa tagatattta acactaataa cagaggcagt
     1201 agaaatcact agctattaga taagcaaaat agctattagt ttttgtcgtc cctaaaatta
     1261 attgctctat gcataccatc acaaaacggc ttattctcgg aagcaccaca acgacaaaga
     1321 gtataatgtt ctcttgatga tccttctgca aactgaatat tatctgcaat aagttctaca
     1381 cctccggtaa taacaaaagg tccatctctc gagacagtga ccattggcct tctgtcattt
     1441 tggtctctat attctacgcc attaatagaa taactgaggg caccggaagg gcattttctc
     1501 actgtgttga ttatttcctc tactgtagca ccatcagagt caatccatgg tctttcattg
     1561 atcttgaaaa cagatggtga attgtttaca cattccgctg catgggaaca gagtttccta
     1621 ttatcatgaa ttgttatttt cttgccaaca taacttttcc ttttgtcttt gattatattc
     1681 ttcttatcat tgtctgaagc agtagctgtt actttatttt cacttgaaaa accaattgtt
     1741 ccatgtgtac catcacagaa gggtttattc tttgatgctc cacacctaca caaggccaca
     1801 ccaagcatag ttgaaagtgg ttcagctttt gaattctgaa gattttcaac aattttaggt
     1861 ttcatatcat ttaacagata ataaggacca ttactaagag gtagaatctt tggcttttct
     1921 tttaattgat tttcttctgc ttctctacta ctatttgaca taattgttta tcacccaaat
     1981 cttatttcat gaatatctcg tcaacataac accatttcca attttcgccg ggttcaaatg
     2041 acttaatgat aggatgaccg gttgctctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnaagtgt tttgtgccat gcttatttat cgaagaatta
     2281 caacagccga catgaccgca tgataaacat agccgtaaat gtacccagtt agatccaatc
     2341 ttttcacact cttcacaacc ttttgttttt gcttgtgcat caaggtgtac ttgtttaaga
     2401 tgagtgcaag atttcataga tatgtaaaat agtggaatat tattatattt aagttcaata
     2461 gtaatcttaa tactatttag taggttcgac aggacttgct cagtccgcag gtactactat
     2521 atttaataga tttgcgaggt taagagcaat aatgaaacgc gaagatacaa cagaaaaaga
     2581 aattaaaaaa ttgactgcac ctgtaactat tggttctgat cacatccgag gctccatcga
     2641 tgcaccactt actctagtag agtatggtga ttatgaatgt cactatactg gtatgaatca
     2701 gtttgagcaa gatatggatt attctatatg acgtaggccc attacatttt ccgagatttt
     2761 gttaaggatt tgaagaagct ctttaaactc tgatgcacgt aactgatggc taaactcgta
     2821 atttacttgc tttacaacgc catccgctac tgtttttagt gcctttccct taggagtcaa
     2881 aaggactcga acgaagcgtt tatcatctgg attgtgtcgt gtttctatat atcccctgcc
     2941 ctttagttca ttaatataca ccgtcataga ctgtttacta aggttagaga aggtcataat
     3001 atcggaaatg ttttgattat ctttatcaaa taatggtata aaaacagctc caaaggatgg
     3061 tttaagatcg tcgtatcctt ttgatttgaa aatttccatt agacgctggt tccaagcgcg
     3121 cgaagtcttg atcaataaat aacctaaatt ctgcgacgat gcgtatttca tactattata
     3181 tatcttatga cttatgacta aactataata actagattat ttgagtcgag attctctatc
     3241 taataattcg tatatgtcac atcgatatat ttatattagt actataatcg tactaatata
     3301 gtatgaattc aagaacagca tcattaatgg aaccaatcct tctctgtatt ccgttgcaat
     3361 taggctgcca aggttatttg ctatacctgc aaacctgatg gacttatctg cttttagtat
     3421 ttgtttgcag aacatggaaa gatttgtatt tcttgttgct gctgcagtat gatcttcttg
     3481 caatgacata atcatcgcta atgtacatct gtaatataat aataccaaga attcactgta
     3541 ttgcagtgca gtcctcaatc atataacaga ttattagttt aattatggat aataaagatt
     3601 agttatcatg atatccgaat tacaaggaga aaattacatt aatctggaaa cttacgaaaa
     3661 aaatggacaa ccgattcgga cgccagttgc gtttgttcaa gacaatgggg tgatttatgt
     3721 caggacggat aagaattcag gcaaattaaa acgttcacgg aacaatccgc atgtgcgcag
     3781 tgtgccatgt gatattagag ggcggccgga aggcgaatgg gttgaaggta cgatccagat
     3841 cgctagtgaa tatgaatcag aacgtgctaa acaacttcta aacgaaaaat acggcannnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac ttctaaacga aaaatacggc
     4021 agcggcttcc gtggaatgct gcttaatata atgtacaagt ttagaaagat tgatttcgtt
     4081 tttctttcaa ttcgctagca ggtctgtttt gactgattaa ttgggattca tccccgcgcg
     4141 tcaagatgcg agccgcttcc gttatcagat tccaactgtg tatgcaatgc attgcaatat
     4201 catgcacata cggaaaacag ataacgctga gttatattaa ttcacttaca gggagattat
     4261 ggtggtggcc gaagtttaaa taatagaggc gttgtatttc ccaacaggaa atcttgcttt
     4321 tctttgtgat tctacaccag agcaaataca atgagagttt gaatcgaact ataatagggt
     4381 tgaaatagat caccatggac ccttacgaaa gttgtaaaat cgccgtttaa atcggtatgg
     4441 tatatcaaat gtatgtaata tcgtaacatc tcaaacctat gttataattg cttctccaaa
     4501 cataaccccc accaagtaac agtgtgtgat aaagaagaat gacaaactgc ccaaagtgta
     4561 accatgatga atttttacat aggagactca aagtgcatat agcaggcaac cataaaactg
     4621 aagaatttgt tcatccagta cgttgtattg aaaaaggtcg aggctgtact gaacctcaac
     4681 taatacaata gtagctatcc caatatttat agtaggaact aacgtattaa cgttcgtgag
     4741 taagttaatg aattgtccaa aatgtttaca aatattacat tatcatagcg gaggaatgac
     4801 ctgcagagct tgcgggtttt ctaaatctca ttatgaaaca ctgatgtgta ctatatgcaa
     4861 atatacatta gaagaagcag aaaagactac aggggtttgt gtattctgta gttataaaat
     4921 gagtaggcag tggcgcctag gaaatggcaa gatcaaagat aatgtaagcc caatacccat
     4981 atagataaac agcctccatt atgacttaat agaaactcta ggatctttct atcgtttata
     5041 acacgaagta atttagattt tctactgcct tcgctaccgc caacaccatc gccatgatga
     5101 ccagagagtc cttcctttat tcctatattt ctaatactct tacttatcat gtagtctaat
     5161 acacttctga cgctagtctt accatctacg gatattaatt gagaagagag ttcctttatt
     5221 gatgtgctga gattagcttt tgcaattagc ggtagaatat ctcgtattgc aagtgatgct
     5281 gttactacta ttaatgaatc gccttcttcg tcctccatcc tatcatcctt ttttgctagt
     5341 atgggaataa aagcatcccc gcaacgatat cgctcctaaa aaagggttca tttgtatatg
     5401 aaaagagtaa cccttaaagt tttcgtggcc ttcagtacag tgccactacc tgtgttcgtc
     5461 atggcaccat ttttggtttc attcttcaca ggcctaacta atcttccctg gctgctgctt
     5521 cattttcgag atttcttccg caatattttc ttcaaccagc cttgaattgt tccgttcaac
     5581 cttttgcagc gtttttgaaa aaacaatttg ggtaaattat acatcttatc aacaatgaat
     5641 ggatcatccc tagtcgcgga aggggatggc cactaatctg ccattagttg atacgttact
     5701 cgtccaaata aaatagcgtc tactttactt agtagatcat tggcatatcc attaaattct
     5761 tcatccacga catgccaatt tagctctctg tttggtcctt caaaaaagtc atcaagtgtc
     5821 tcatcttatt ttttcaaatg ttaagtgcta agtaaatact aaatagacta attactagaa
     5881 cggattacag atgattaata tgaggacact agaggacctt ttggacaaaa ttgtttggtg
     5941 atgctgaaat acgagcgtat aagatcgacg gattattatt agttgtgaga cctgagacta
     6001 gtttgtctag tgcagtaggt actatggtct gaaatgaagt attcaggagt aaaaatatga
     6061 aattttcacc tttatacact tattcgtata tttcaatcag cagtttcgtt ttcgggttac
     6121 atcatattag caatgcgagt ttaatgtaac tagagggagg tcaggaatat catttacatt
     6181 cttgcgacca tatttcttca tgcaaaactt tatcaatttt ttggataata cgataagtat
     6241 cagaattatt caagatttta ccacacaaaa tacagtaaga tgtttcacat gtaatgcatt
     6301 ttgtgtactg agacattttt tcatagtctc ctatttgagg gatgataaaa tcgtgttcat
     6361 ggtgcaggac gttatactca gtgtcttcat attttgacac gttaggcttc ttcctgtaat
     6421 tacatgcaga gtgttatatc aaacctatgc ttgcattgca acgcactgca tgcctacttt
     6481 tcaaatacaa gacttctccc atatttctat atgataaaat taagttacgt ggactttgta
     6541 ttgtatttat caatatcagc tcttttagta tttgggctta atctcgcttt gattcccaat
     6601 aatacaactg taggagcaat attaaaaaca atcctaccta taaacataat aaatggtagt
     6661 agtggaacag gcacaacaat aacaaccgct aacgttgcgg cgacggagct gacggacaag
     6721 agctcggtgg cacaaatggc gaccgcaagt ctaggagcac ctagcaacaa gacgttttac
     6781 ctattcaatg ctgacacagg ttcaaataaa aatcaaacac acataccacc tgacacattc
     6841 acacctgacg taataacagt caataagggc gataatgtaa acattgtata ctataataca
     6901 gaaccaataa aaggtggaga tccacacaca ttcacgatag gagcaccata cgacataaac
     6961 gtaaatgtta atccacaaca acattcaaca atacacttca aagcaaacac agcaggagca
     7021 tttcagttct attgctcagt gcaccttcca acaatgcgtg gtgagttagt ggtcctaccc
     7081 taaaaagaaa ggttcgaaga taagacattg atatatggtt gcagaagtta tggagatgaa
     7141 aggaaataga gaaatgacta ctaccaaacc aacatatagg aaattcctgt atgaagtaac
     7201 tggttttgta tgggctaaag atgaatatga cgcgaatgcg ttatttgaaa ctgctatact
     7261 agaagtgata ggattacccg acaagataca agggcacatt gaagtaatcg gaaaacggac
     7321 agaacggtca ataacggata cagacagaaa cagtgaataa cggatcaatc ggaggcgaag
     7381 ttatgaattt acgaatattt agcttttcta ggtgcattaa tagtagtcta ccacggccga
     7441 attcaaagat gtgatatacg atttggaaag attttgagaa taaagctgta agcactctta
     7501 ggagaaacta tgtgtcctct tagtactact cttacattac aaggaataca ttaagaattg
     7561 cttctgaata gatcagacgc atctgtctgt ttgtaatata tattcaaagc cctaacttcg
     7621 gaactatggt tatccctatc cagccaaatc tcttcacaca gtttgggtgc cattgataat
     7681 tccgaggccc aagaaa
//
LOCUS       DAOR01000257            3221 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_55647, whole genome shotgun sequence.
ACCESSION   DAOR01000257 DAOR01000000
VERSION     DAOR01000257.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_55647"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1008 a          612 c          626 g          975 t
ORIGIN      
        1 agggtataat agaaagcacc aaggctggca caacaatcac tccaaaagga aaaaggatat
       61 ctgaacaatt tttctctgca atacctacag aaatgagctt atcaaaatgt tctgttgctt
      121 taggtaaatt caactatgca gtattgttaa aacaatatgg atttgctctc aagtcaggaa
      181 tagaacaaag agatgctgca ataagaatgg gtgcgatagg agctacttca ttactgttta
      241 tgcataacag ctttgtaatg cctggtactg attatgatcc attgacaaaa gaaccagata
      301 ctacgagact attgcttgaa aaactgaaac cagaagatgg tgatgccata gttattggaa
      361 gcgacatgga ggatgaaaga agagcagagc tagcagcaaa gaacgctgca ctaacaacta
      421 taattgctca tgaaagacat aatcaaatgt agcttgacac ctaagttata catcacagat
      481 gttcgtctac ttaggcggtc tagctatcta caaatttgaa tttaccgtga ccatccccct
      541 catctcactc tctgcggttt atgcacatgg ccggaaatct aagccctgag ctcacttacc
      601 ttcaaaagac tggctatgta gagggttccg acgaagatct gcagctgaca ggcgaaggga
      661 tgaaaactac tctttggata tttcggaagt ttctaacctt tatcaaaagg gaataccccg
      721 taatgttatc ctcttggata aataatctcg agctccataa gagcaatacc tgggaactta
      781 tacgagactc ctttttttat attagaagag agcctctaat gactgaagcc ttcaaagatt
      841 atttgaggga cttggagagt atagaaaatg ttcttagcat tgaaatcgag gaatacgata
      901 taggcacgct cattgatgac atattcttga atatgtatga tgtgaataaa ttgtttgagc
      961 acaaattcag ccataagctt ttttgtcccc caataccggc tcagtctgtt ctgaggaaag
     1021 caactgaagg aaaggaaata agacttacag attttgtcgc gactcttgga tcggtcatag
     1081 atggaatatg tcattatgaa atcgatagtt tactaggttc aacaaagcag gttaagggtt
     1141 ccatcaacaa gattcaggct atgctagacg ataagaagat aaactataat gctaatttta
     1201 tagcaatact gagggcactg cgtggcttaa ggaatacgac atttccaatt catgacacgg
     1261 gacctgagat aataagtcat ttacaagatt tgaatcttag cttccctcta aaaaactata
     1321 aagatgccgc actcaagatg ttgcaagctt ttaattcgtg cctcttggaa atgaaagttt
     1381 ggtttagata ataatccgga aaaagctctc catttcgcag ccttcaagag tcgtcaagta
     1441 ttccaatcta aatgtgcttc ataccctttg acaaacgcat caaggttttg gtatttttct
     1501 ggagaagtgt taaaatggca catattcttt gacctcttta tagcctcgtc gtcagggttt
     1561 actacaatta actcttttaa ggtattttcc gagaatgctt caagaaacat ctttttagtt
     1621 aagaagtcag taggtgggaa tgaataacca attattatta accttttgca caacgaaaga
     1681 gctgccttag cttttttcca tagtggtaac agcttacgat agataggatc gcggaattgt
     1741 tcttctttat gaaggagtgg cgcaataata ataggatcaa ttggccattc atttagttca
     1801 gggtagtctc tccaactcca atcaccttct ccaataataa tatgagatct ttcttctttg
     1861 gacaatacac gttttggcgc tccgagcatt ttagggaatg agtcttgagg gatgtatctg
     1921 aaccagtttg tagaaccatg tagcttaaga atataccaag aataaagatc atgattcgga
     1981 taagaataaa atttcttttc actgacatat tttctacgaa ggtgattgcg aatatctcca
     2041 tcatgtatca taacctcatt aaatgtaata gaatatccga gtggcctatt ccacttccat
     2101 gtgctatatg ctaattcttc gacggttatt tcgctcttgt cgtgaagcct tcgaataggt
     2161 ggtttttcag attgatttaa atgggaagca ttctcgatcg aagcttccaa aaaacaatca
     2221 taattgaacg taataatgat gggtttttcg ttgtacacaa tttgtccaag agccatcatt
     2281 gcagagtgtg tctcgatcct tcgaagccgg tgagaacttc agttagaaat ctctttaatc
     2341 taaattggat atcgaacaac tctctagtcc tttcaatatc tccttcgtct tttgctttcc
     2401 tgtaatgaga ttcaagtaaa gtaaagcatt cttctaaatc aaacttagaa gttgcgaggt
     2461 catccttatt cttcttccag aatttttcga tataagcata cactggctcg attttttcag
     2521 tgtatttttc actgttaaac ttattcattt ttaatgcttc cataaaaaaa ttgcatatca
     2581 gcggaggcct agccggatga gagacgtcta cgaagtttat catggttttt gccatcctct
     2641 cgagcgtttt gatgaaatgg ttaggcttct ctttgacctt tctagcagtg ataggctgac
     2701 gctcctattt gcaataagaa aagaagaaaa tctaaggctc actcagctag ctgaaaagat
     2761 taaagctacg attcaggaaa cgtcaagaca cgtcggacgt ctcacggaag caaacttaat
     2821 agagaagaac tctgatggat actacactct tacttcttac ggcagactcg ttttgcttct
     2881 cctatcttca tatggttttc tttctaagac tagagattat tttctctcac atgatatatc
     2941 cttccttcct caagaattta cggagaggat tggcgagtta ggctagattt tttactctgg
     3001 gtctcgatat agcattagat ccgactgcaa ccccctctcc ctgacgtcct tcaacagata
     3061 tatcgataaa gatcaggcat tcaattctgt atggatgaat taatagagct cgaacaaaga
     3121 attaagcaca aaatcgccca aacaaaagac aaaatgcata agacgcagga aagggcgatt
     3181 actgatcgtc tgtggccaga gatagagaca ttgaattggg t
//
LOCUS       DAOR01000258           39537 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20757, whole genome shotgun sequence.
ACCESSION   DAOR01000258 DAOR01000000
VERSION     DAOR01000258.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 39537)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 39537)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39537
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20757"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    16670..16766
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17364..17570
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    30321..30556
                     /estimated_length=236
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11760 a         7798 c         8342 g        11097 t
ORIGIN      
        1 cctgtgagtt gagctacaaa ctagctattt gaaaaaaggc tatataatgg caagataaaa
       61 tggaaacacg gctaacaata atcacctaaa gccaaactcg agttcggctg tcctgggcta
      121 ttatttcgaa aaaatattga ggacaaaaag ccggtttata ttatagtgtc ccgtcatcta
      181 tccatttgag aaataaagaa aatgtgatca tttttgtcct attattattg acttcaacag
      241 ctgcactaat actagtaata tcaattaacc ctgccaacgc cagtacctcg accgcattgt
      301 cgaggccaca cgttcttcgc tacgctgcgt taggaatagg tgcagcagtt atcatagcag
      361 taggtgtaat catatatatg aaaagacgcc gtagtatttc tagccgagac gccaactaac
      421 agagatacgc tttattgcat tgactatcag cagggatcat actggaccaa gcctgtctta
      481 tgatcatttc atatctaaca ccctgcacca ataaagaccg acggatggca aattactccg
      541 gaaggtgtgt tgtgtaagaa ttgtcgcatg gaatttgtta cttactttgt caactgccta
      601 gcttctcaaa actctcactt gcgaatcgtg taagaacact ttttgtctaa cactgatatc
      661 taacccatat cctagaaaac tgtcgtcaag caatattcga tttctggaat aactaacatc
      721 cacagaaata atgacatgga tatcacttga aatcttggcg tgattgcaat acgaattgac
      781 tggttccatt ttgctattgg tttaaacata ggcctgcttg caatttttta aactgcggtc
      841 tatgaaagca gtctcttgct actatcaaac agggtttgct gtttccatct aaatatttgt
      901 acgagcataa ttcccacact tttataaacc acggcaaaca ctagaacttt tgacccgtta
      961 aatggaaaaa aatgttgatg gaggagcact agcgacagta aaaaagatat tggtaccaat
     1021 tgatagatct ggatataaag aacaaattct tgcatttgca acatccctag gaaaagcatg
     1081 gggagcggaa ataacagcca tacatgtaat tgatccgggc cgcggtgtac cgggcggtag
     1141 ggttaaagaa aaggagcagg agagagaaga agaagcaaag agggaggcgg aagatttact
     1201 agttcagtca gttgaccctt tggcacagaa agaaggagtt aatataaaaa aagaagttgt
     1261 tgaagaatct gattcagttg caaaatcgat aattggctat gcaaagaaga acaatatgga
     1321 tgttatcgtg attggtacta agggtatgac tgcagtggaa gatttttttt taggaagcgt
     1381 cgctaataat gtcattcgtc atgcacattg ttcagtattt gcaatccgct gatgtctcga
     1441 atgattgtgt tcaataaatc attttgagat tgtataatgt tgatcgatct agaaaagata
     1501 atgaccgcgt atctgtagat aacgtaatat tgttccgaaa ggattatgat cacgtacgtg
     1561 aagacaattt atgcagcaat tacgcaaaat tggtctcagt ttcaaatacg cttagggcaa
     1621 tttccaatga tacgtctgcg gttcttttta ctatcattac tatagctctg tggagacgat
     1681 agcatcctaa caagcagaac tcacgagagt aatattattc agagatgtct ggtttaatta
     1741 atgccggtct cacaagaaga aagaatggca aatattttct tcattcggta aaatagtttt
     1801 tggagctcaa gaattgaagg gatcggaatt tccaactgca gagcgtagca agatcagcct
     1861 tgataaacaa cgctgagatc gaggagatgc tggttagccc ccagcacaac aacaactact
     1921 aataaggaaa aagcgtactg caaggagcct cagcggcaac aaaagcctca attatgcaat
     1981 tcaattcatg aatctggtcg taatgataag cgttcccgat cgcagacgac acaccaccca
     2041 aatttaccgt attcgacttg gttatatata ctgccgagag ctagtataat tgcagacatc
     2101 aatgaagata ctttctgtga acgtgtctct cccgcgcgag gtactcttta atgaccaagt
     2161 tgttataaca ggaattttca aagaacctgt acaaggtcgt attaatcttc acaaacttaa
     2221 cctagagggt gataagcaag cagatttgac cgtacatggc ggaatggaca aggctgtcta
     2281 ctcataccct gcagaacatt atgagtactg gcaacgggaa gtgcctaaca cacgaatggc
     2341 atggggcatg ttcggagaaa atttcactac cgagggatta atggaagatg ctgttaatat
     2401 tggagacagg ttccaaattg gttctgctaa attagtcgcg acgcaacctc gaatgccctg
     2461 ctacaaactc ggagttaaat ttggacgaat ggatattata agacagtttc tagctagtgg
     2521 tcgtcctgga atttatttta gagtattaca agaagggaaa gtaggagctg gtgatgcaat
     2581 acatcttctc agtaggaaca caaacaatat cacagtcaac gacatagtac agttgtatac
     2641 tcgtgacaaa aagaatattg agaggatgca acgagcaact cgaattaaag acttacctgc
     2701 aggatggaga ttccatttcc ttgaacgaat ttctgaaatg gacaaatcta taggagtcaa
     2761 tggttcaatt gcaaagtaaa ctggttgatg gtttcggtag gattggcaga aagttaagga
     2821 tctcaattac agacagatgc aatatgcggt gcgtatattg tatgccaaac ggcaataccg
     2881 agtggattga acaaaataat attttaacct atgatgaaat cgtcaaactt gctactattc
     2941 tagctagact aggtatcgat aagataaggg taacaggggg tgagcctcta gttagaccta
     3001 aagtagaaga tctaattaag acattatccc atatcgatga aattaaggct atcagtatga
     3061 cgacgaatgg cctgttactg agagataagg tgatgcagct cagagatgca ggactgtcaa
     3121 gcataaatgt cagtttagac acattcaaag aagataggtt caaagcaatt actggtatac
     3181 gcggactcga caaggttatg aatgcgatac gagtagcaga tgacgctgga ctgaaaataa
     3241 agataaacac cgtcataatg agagggtgga acgatgatga aattgcggac tttgccagat
     3301 ttgccagatt tacaggctat actgtacggt tcatagagtt catgccgtta gacggatcag
     3361 gtatatggga accaaatcta gttgttacta agaacgagac cattaacaga ataagcaaag
     3421 tcgtaaagga gcttgtgcgg gtagacaaca ataatagttc tgaacctgca acgctttatt
     3481 cttttgttga tggaattggt actgtaggat ttattccgtc aatatcagag ccattttgcg
     3541 gtaattgtga cagattaagg ataagttctg atggcagatt attgacgtgt ctttatgaaa
     3601 atcctggcta tgatcttaga agtatgttac gaggtaaaag gtcagagaac gagatcaccg
     3661 aatatattct ccaaagtgta aagaaaaaac ccgaaggaat aataaagatc atacggacaa
     3721 aatcactgaa gccaacgcta aattttatgc atagaatagg tgggtagtta attgacatgc
     3781 atcgcatctg atcaacgttg cacccttgaa tgcgaatcag gataatagct cacccgagct
     3841 ctgctccttc tggccctcag aacgttttat cagcaatcct gcaacagttt cgatttctgt
     3901 gcaatgatgt aacagccaaa tagttggtgg tagcaaaggc agacattaat gggaagacga
     3961 tcgacgacaa gggtagaatt gatcaaaata cttctgaaag acagattgca aattaaggat
     4021 atcaagaata ttccaccaat gaacttttct atttctgata aggaaatgca tgggtttggg
     4081 tttatttatc tccaaaggca ttgtcgaagc tcatggtggc aagatgtggg ctgagaataa
     4141 tgctgatggt agaggcgcta cattcttttt tagtttgctg agagctagtt gatgctgtga
     4201 aggcattcga ttacagaagc acggctaggt cgtgaaaaag atggttgttt gtacatctat
     4261 ataataatat gattttatta cggttatcac gtcatgagtt atcgtagtaa tgtctaactt
     4321 aacagcttaa cccaaaatca agaattaact cacctaccca aagcgtttta aaaagggtgt
     4381 ctaacctgtt tctaatctgc agaaactcta tagatgagga ttataggctt tgaataattg
     4441 gctcgatgaa ttagcttgag tttacctaga ctagtggtat cagtcgaaaa ccttagagct
     4501 gatacattta ataccacttt aagcgctgat gagagagggt gaaacccgaa taacgaaagc
     4561 ttttgattta atgaccacta atgtcgtggc tgccagggag agcactacgg gtacggaaat
     4621 aagtaccaga cttctcattg gagaatttaa cggcgttcct gttatcgaca ataatatcag
     4681 agttacagga atagttactg cggccgatat cttgcgagca gtacgacaag gcaagaacct
     4741 tgatagcctt cgcgctacag atataatgac cagaaacccg gctgtggtaa agcgggatac
     4801 cgagatagac gagataattg acgtcatgct ccaaaaggtt attgtccttg tacctgtcgt
     4861 agatgacgag aataaactga taggagttgt ggcgaggctt gacatcctga gagaaaagct
     4921 aaatgaaaaa tttgtcacca taggtgaaag aagcggcctt tcaaaaacat gagttaggct
     4981 ttatgctttg tattacctcc ttagtattga gccaaaccac cccagcctag ctccgcgctt
     5041 agaaatgcag atgagtatat tatccgcagg ttctattttt gtatacttct cgatctctcc
     5101 cattagtaat ctaattttat aaatttctat gttctcacga atgagggaat gtcatccgat
     5161 aaaggtaccg cagaggcaac cttgcaactg aatctgaaac tgaaatgaca gtgataacgc
     5221 ctccttcgga tgctaacccc gaagaagatg tatttggagg tttaatattg aaacacgtct
     5281 accttatcgc cggtcttgtt gcaagacgtc atttttgggt ctaacgttgt taccgcaggt
     5341 atcgataggg tgacagtatt cataattacg gtattcataa tcaatgcact gatcctcaaa
     5401 ggtatattga actatctgaa gcgatcttcg aataaacttg aagttagtat ggaagctgac
     5461 gatcttgatc atcgttcatg agctgcaact gcatttgtta caatggtagt gatcgacaat
     5521 gaaggaaaaa gccacaaaag tagctgcgga attattggaa agagacgacc ggcggcgctt
     5581 tgagcaggat caggcacgaa tgcttgcacg ctcaaaagaa attgggagat actaattttt
     5641 aagaataact aatgataatc cttctccttc tttctatcta ctgcgttgat tgttttcgtt
     5701 ttttccttgt taagttcatc cttactaata ccgctcgcta ggattattat cttgtcgtca
     5761 cctccagtaa aaatagcagc gtgagtagaa ttttttaaag tttgaagcac agcgtaatca
     5821 cccacctgga attcttttaa ccctagtgga attagggcat catagtcatc gatatctaaa
     5881 atagtcacct tgacaagtcg ctcagttaca gattctattt ttgcgaacat atcgacaaaa
     5941 gatcaaagtg aacgctgtta aatacgcatt tctaaaaata aaagttgcgg gtagatcaaa
     6001 tctggcttgt gttctcgagt gtatccacta aacatacaga gtatggttat gtgtcttttt
     6061 aaaccaccat aagacacaga caacgtaaca gatagagcca atcctcctat catgcaacaa
     6121 cacaacacag aaattgtgtc gcatgatacg gaggttctct atcgtttggc tggtatgtgt
     6181 tggttaatat tccatactga tattccctat ttttactttg ctatttggtc atttctttgc
     6241 tttctgccta tattcttcta attcttctac aatctaactg aaaatcataa ggattctaat
     6301 gtcgtcaaga ttattgtctg tcccagaaaa ggcatgcttt tgtatttttg tggttagtgg
     6361 tgcttaaaac tggagctttt gaataaattg atttccaacc tgccttgcaa atcttttgag
     6421 aaaaagctac cgtctacctt taggtttcat ttgccaatgc gttcctctaa cagtctcata
     6481 gcgcttgtgc agtcagagtt ttagacaata aatctctatg acgattatcg aagtctacct
     6541 tctccatcac ataaaacaca ttttgaatca agttattttt cgtggaatct gcaggctttc
     6601 gattgtgtag atctttttcg gatggctcca attctactaa ttgtttatac ggaaattgca
     6661 cctcatgctt tgcattgtct aatgcagcgt catagtcaga ttgcatgaaa tagcctaacg
     6721 agttctatac tatattattg gtcattgcag atcaaagctt cttattttcc tttaagagac
     6781 gaattacagc ttcttcaagt aacgcagtag cagtggtatt cctttccaac gccagcatct
     6841 taagtgaacg aatgatatct gtattaaatt tgaaggtaaa cgttcgggta tccttaccct
     6901 ccttcttagt aactgtcaat tgttgagctg atttagttat tgtattataa agatatattc
     6961 gttaacgaat aataaagctt tatgtattgt caatgttgct ttatatgcaa ctaaacttta
     7021 tatatttact actttatgta taactaatga aagcgtaaat cgaatgcgaa aggttccatt
     7081 caccgaatgc tagggaaggt cggtaacacc ctttgttgta agtgtatagt atgcctcttg
     7141 tttgcttgaa ggctgtaata agactgcctt gaagatggat ttctccaaat ttgtagcttt
     7201 tagcatcaat ttcatgtgtg cataaatgga gacagaagtc cccatgagct ctcgttgttg
     7261 atcagattgt tgcgtctttg acctattggg attttctacc ataattatgg aagtctttac
     7321 ttggtttcca gcagtaatcg cagtacgaac catatttgtc attactacgg cgcagcgaag
     7381 attaatagcc gccaccgaca actcatgaag atgtttcatt agagctaggt gccttgccgc
     7441 gcctcgatag tcggtcgtga ataatgacgc aacaccatct attattaaca gacgaggatt
     7501 gattttggag agtctgttga tcgagtttat ttgatcagta gatgataaag atctcacaac
     7561 cagaattttg tcaagaatgt cggtcttcat ttttgccatt ccagcaatct ccgtaattct
     7621 ttcgggccgg aacgttcctc ctgtatcaat aaaaacgacg cttacttgct gacgggtttc
     7681 aaaatattct gcagttcttg cgcagatagt aaaacataat tgtgacttgc ctgtaccact
     7741 ttcgccgtaa atgtctgtaa ccatcccata gtgaatcccg cctccaagaa ggttatcaat
     7801 tgctttacat ccagtcggta aaacatgtgg catcagtttt tcagtactgt actagtgaaa
     7861 taaactgata tggctgtttc gcaagaacag gggaaccttt ttattgtagc tacggtgctc
     7921 tcacatccaa agtgattagt tggatttgtc gtcttcacct tcatcccagc aaccaaaacg
     7981 accattatct actttgcagc gtgcagtcaa tcgaaaggtt gccgtgagat tgaagagcga
     8041 gatcgaatat cgcggcaaga tgagcaacgt tgattcttat atgaatctga ttctggtaaa
     8101 tgctgaagag ttcgatgggg ctgacgtttt ggcaaactac ggcaaggttg taattagagg
     8161 aaataatgtc ctcttcataa agcttgagaa agaattctga cgagatataa gtgagcttcg
     8221 taaattcaag ctataaaagc agattcgtat ggtacgaaga tcaattttca cgtccgaaag
     8281 cgtaacggag gggcatccgg acaaattatg tgatcagatc tcagattcgc ttctagatga
     8341 attcctaaaa caagatcctg actccagagt tgcagtcgaa gcaatgacta ctactggaat
     8401 tgtattcgtt gccggtgagg taacctctac tgggagattc gatctgcaac gcaccatccg
     8461 aaggacaata aaggaaattg gttatgataa aacagaatac ggatttgatt cggaaacttg
     8521 ctcagtcctt gtatcgattc atgagcaaag tccggatatc tcgatgggta tcaccccagg
     8581 ctacaacaag gagcagggtg caggagatca aggtttgatg tttggttatg cttcagacga
     8641 gactagagag cttatgcctt tgcctatatc gatggcgcat aaattatccc tgaaactgtc
     8701 acaagtcaga aagaacaagg aattagactg ggttaggcct gacgggaaat cacaggtatc
     8761 ggtagtgtat gaagattcca agcccgtaag gatcgataca gttgtgattt ctactcagca
     8821 ttcaccggat attcatctac aacagttgag agaggaaatt attaacaaag tggtaaagcc
     8881 agtttgcggt aactgggttg atgtcaaaac aaaatacctt gttaatccta ccggtagatt
     8941 cgtaattggc ggaccacaag gcgatactgg actgacgggc agaaaagtca ttgctgacac
     9001 ctatggtgga atgggcaggc atgggggtgg cgcgttttcg gggaaagatc catctaaggt
     9061 agatagatca gcgtgttatt tagcgagata cgtcgcaaaa aatgttgttg catcaggctt
     9121 ggctgatagg tgtgaggttc aaatcgctta cgcaattggc attgcagaac cggtctccgt
     9181 aatgatagat acatttggca catgtaagat cgcggaggat gaaatcgaag agcgggtgaa
     9241 aaaaagcttt gatatgaggc cggctggaat tatcagcacg cttgatctca aaaaaccgat
     9301 ctatagaaaa accgctgcgt atggtcattt tggtagagat gaaccgggat ttacatggga
     9361 aattacagat aaggtctctt tgcttactaa gtagaaatct ttggggccgc agaatttttt
     9421 gtggtagtag gtcgtgatga tgacgagttc agaacatctc tcaaggcgtc atccagattt
     9481 ttacctgccc cgaatctacg gttaaagaat cgtacaatat tgaatacatc cctttttatt
     9541 aactgctgag cgttgggatg gtccgtcgtc actgcttgag gccagtcaat aatccacgca
     9601 tcgcatgagg tttctcctac caaaacgtta tattcgctta gatcagaatg tatgatatga
     9661 ttcttgtaag ctattcctat gcttaacaaa atgttttgca atactgtgct tggctgttca
     9721 aggtctttca cacttacaag tctagctcca tttattcggt tcatagcaat gcagtgcatt
     9781 gtccggtaca aaggccgagg aaggctgata ccagtatttt ttagacgctg tagcatttct
     9841 acttctttct tggctgcgct aatattgacg agtaaccagt gatgtattct gtcgtgttcc
     9901 tttaatgcaa aagacctttt cttggttact tctctaaagc ttattctgcc gattctaaag
     9961 aatttgacag ctctttcctc ttgtcgatct gttacggcct cgtacacgtc cgattctttg
    10021 ccgacaccaa tgggttttcc gataccactt atgatatttt tctcagccag gatctttaat
    10081 gcgaaagtat caagtcctgc attgaggata gtaaaacccc tattattttt gttaattaga
    10141 ttcatttctt gtagtccttt cactctataa tcgactatgt ccccatgcaa ttttgagtag
    10201 cccactaact ggtgccttgt aagagtttca tgatgttcaa gtccggaagc aaaaactctg
    10261 aggagcttgt agtcttcccg ttccaaattc ttgacgacgt tcgctgcaat ttgtacggag
    10321 gacaatatgg agtacgtatt gtttttgtca atatgaatca aacgtaaaat actccaacct
    10381 tgggattcag gcaggtcgta aaatcactca aatcttggcg tgtgttagac atactgatgt
    10441 taggcatcag ataaaaataa taaaagaagg atcgggtcta aataagcaat tgtcattgtc
    10501 tctgagcggc aaggtggccg tagttaccgg cccgactaga ggtattggat tagcgatagc
    10561 aaaagaattc gcagagaaca acgatgcaga cgttgtgatt tgctctggga gtaagcaaag
    10621 agcagtacaa ctagctaaag aaataagcgg caaagcattt ggtgcaaaat tcggtataac
    10681 tgatatcttt gtattggagg aaataatagg ggttcgccat cggatagaca ttttagtaga
    10741 caacgcaagg tgtcatttta atagcaagac ttgcagcaaa atacgaagcg agcgcgatga
    10801 ccaagcatgt acatggtcgc aataccacca ttcactgctc cgaaacattg ctaaggacgg
    10861 aacctacaat tcaatgtcta ccgacaaaaa aatgaatgcc atagaggaac cttccatgaa
    10921 gagattggct aaaccgggag aagtatcaaa gatagtggcc cgcgctgcga gcagtggttt
    10981 ctcgtttgca actggaaaca ccctagttgt agggggcggt atcgtgttat tatgatattt
    11041 tttgatggat gacgaaaggc tattggacac aagtgaaaat ggtgaagacc aattgagagg
    11101 agaagatacg gcaaaagcag atacacaaga tacggagtca caaaaagatc tcgagctagc
    11161 agaaagggag cagcagctgc ctctggaaat tcaagaaaaa aggaatcgag gtgctctgag
    11221 gacgggtttt acgactggaa cttcagcgac cgctgcgact aaagcagctc ttatcgcgct
    11281 gctatcccag aagaccgtca acgatgtttc ggtgtctttg cccaagggga aaacaatgaa
    11341 catcaggatc gcatggacga acactacaaa agaacaatat tcggctaccg cagcagtcgt
    11401 caaagatgcg ggagatgatc ctgatgtcac agatcgagca gaggtttgct caactgtttt
    11461 tctaactaac gaggtcggaa tcgtttcgat tgacggagga aatggcgtcg gcaggataac
    11521 aaaacccggc cttgggatag atatcggtaa gcccgcaatc aatctaagtc caataaaaat
    11581 gatagaaagg gctgttagcg aagtcgcggc ggaacgtttg aaggaccacg gtgtgaaagt
    11641 cgttatttct gtgcctatgg gagaagaaat tgcaaaaaaa actgacaatc ctaggcttgg
    11701 gataatcgga gggatttcaa tactcgggac gactggaatc gtatttcctt actcgacagc
    11761 ctcttttgca gctgcaattc gacaggctct ggatgtagct cgagcaatgg gtgatgagcg
    11821 tgttgtgttg acaacgggcg gtcggagcga agactttgct aaagatcttt tcggtaaaac
    11881 tcttccagag cactgcttca ttcaaatggg agactttgct ggttactcta ttagacattg
    11941 cgcgctaaaa caaatccgga aggctacaat agcaggtttt gtaggaaaat taacgaaaat
    12001 gtcaatggga atcaagcaga cccatgttcg aggctcgcac gtcgacatgg agttcatggc
    12061 gcacgttgct tccgagtgct tcccgaggat tccgatgtca actctagagg agataagaaa
    12121 tgccaatacc gctagacacg tatctgaaat aattacgact tatcaaataa ccggtttctt
    12181 tgatttgatt tgcaaaaaag tctatgaaca tatgcgtgaa tactcgaagg gagagttaga
    12241 attagaagtg atcatgtttg attttgatgg caaagtatca gggcaatttc ctggtggtcc
    12301 ctcctaccgc gcgtaacatc gtgacctcta gtgttggctc gccgccgatt ttaattacac
    12361 aatcccaatc tcttttgctc gcgtcagggt atctgtcttg gtcgtgcccg accatgaatt
    12421 ttgacacccg gagagcagaa taaacgtcga attcgaaata cctaactcaa aacataagac
    12481 tggatttggt cctgatacca tataatgcta ttgttccagt atggtatttc cagtatgtta
    12541 cttgcgttat cagtgccatt gtaagattcg gctatctagc ttggcagtgc gactgaacac
    12601 tatgatatat tgctgagata aatcgcttca ggccgcgata tcgtattggg tattagactg
    12661 ctcgcagata atttgggaaa gattttttat tttctacttc cttattgaac ttggtagccc
    12721 cgatgacagg aaacacggcc gtgatagcca ttacaagaca tggaatcgac atcgcaaata
    12781 gaataatgga gaaaatgcca gaggtacaag tttatgcacc tataaaatat gctaacccgt
    12841 cgatgtccaa catgaattgg tttaaagaac aaacttctca gttgatagca aacctgttca
    12901 aaaaaaatga tgcattgatt tgtatatttt cgctaggcgc agtagtcagg ctaattgcac
    12961 cctttctagt ggacaagaag acagaccctg cagtcattgt aattgacgat aaggcacatt
    13021 ttgttatcag cgctctctca ggccaccttg ggggggcgaa ttcgctcgca aggctagttt
    13081 cctccttttt tgacaactgc cagccggtaa taactacagc agcagacgta aacgagacta
    13141 ttgctgttga cctgttggga agagagtttt catggacaat tgacaacttt gaaaatgtca
    13201 ccaaggtcag tgccttcatg gttaatgaag aaaaaatcgg tgtttttcag gagacaggcg
    13261 aaaaggattg gtggcagaat aaatcgttgc ctagtaatgt aacacttgtc cgtaatctgg
    13321 atgatttaaa gtcatcgcat tttaaaggag cgctagttat ttcggatagg ataatcgatg
    13381 acccgcttat tttagaaaaa tcagttcttt atagaccaaa aagcttggtt gtggggatag
    13441 gaattcatcg ggatacaaac agcggggtta tcgaacacgg tgtgaagaat acaatgaaaa
    13501 atgaaggctt gagcttcaat tcgataagaa atattgcgtc gatcaatcgg gaggcaggtg
    13561 taaaaggctt gcaagatttt tcatccatct acaaaatccc cgtagaggtt tatgacagaa
    13621 acaaactcgg aattataccc gtaccaaatc cttctgagac cgtaaaaagg ttcgaaggca
    13681 ctccgagtgt gtcggaggcc tctgcgattc tgagtgccaa aggcgatttg gtagtgccaa
    13741 aacaaaaata tcccccagat cttactgtgg caattgccag ggtgaggttt tagcagtttg
    13801 aaaaaagcaa tactgattat agatagagga agtcgagagc ctgaagtcag acaagaactg
    13861 gaggatatct gctcgcttgt caaaacaaat ggaggatact attacgcaga ttattgtttt
    13921 ttggaagtac ttccgccctt catcgaggaa ggaatcaaaa aatgtcaaga aaatggtgca
    13981 gatttcataa ctgtgatgcc atattttctc tatcctggaa tgaaattaaa ggattcggtc
    14041 aggcaaagcg ctagaatcag taagattaag aagctgaatt tggtgattac aaagcctctt
    14101 agttaccatt ctatgatgac acaattggta atggatagaa tcacctgctt gaaaaaagaa
    14161 aagagtattc gatattccga tcttgaatgt gatattcttc ttataggcca cggcagtagt
    14221 gataaaaatg ccagagatgc ttttgtttat atcgtcaata ccttaaggcc cttttatcgt
    14281 gcagtgaaat tttgttttct tgaattggac aagccgaata tccagcaagg gctaaaaaac
    14341 ttgcttgata ataacccaaa aattgtcata ttaatgccat attttcttca taaaggcgcc
    14401 catataaaac gggatgtaat tgctgacgtt aatgaagcgc taaaaaaaca gccgttcgaa
    14461 aacgcattta tgacgagtca cttgggggtg gatcagaaat taattaatct tctaatcgag
    14521 cgagcaaggg aggttgagga aaaaagactt ggattctgct gaccagcgac cagaaacagc
    14581 cttcaataga gggatggcgg aaagagcgtt agatatagaa aaaaaaagct ttgagataat
    14641 agatagcgag ataggcgatc atgattatgc tcgattagaa tgggatatcg tacggagggt
    14701 tatacatgct accgcagatt tcgattttgt gactacggct aaaataatat tttctaatga
    14761 tgcgctagtt tcaggcttta atgctatgaa aagcaattgt actattgtca ccgacgtaga
    14821 tatggttctc gcagctttaa ataaaaaatc gctttctgct cttgggctta gtgctgtctg
    14881 ccatatctca aataaaactt tggtagaaat gtcgcgtcga ttaaacaaaa ctagatcaga
    14941 aatggcaatg cgtcatgcgg caaatgaaat gaatgaggga atagtactga taggtaatgc
    15001 gccgaccgca ttatttgaaa cgataaagat gataaatgag ggcgcgacta aacctgcgct
    15061 agtgatagga atgcccgttg gtttcgtttc cgcaccggag tcaaaagaag aactttccaa
    15121 gacaaaaatt ccgtttatca ctaatagagg caggaaaggt ggaagcgccg cagcgtcttc
    15181 tataatgaat gcattaatgc ttctacttca gaaatttgga aaaaacgaca acattgacta
    15241 tgaaaacccc tataaattgt aggatgatat tttttataat atattaagaa ggaaggacac
    15301 aagttctgtg acagacatgg cttcgaagaa atctacaaca attggcgaga ttatgagtga
    15361 aagactggaa acggtcaatg aatctgacac tgctcaacag gcagccaaga aattgaggaa
    15421 tagtaacgtg agctccctgt tagtcgtcga tggcggaggt aagtatgtag gaattataac
    15481 ggagcgtgac ctggtgagac gcgtttgtgc taatgatgtg agcagtagca aagtcataat
    15541 tcagaagata atgtcgactc ctcttattac agtcggttcg agctctctac tacgagtagc
    15601 agcgaacgta atgatccaac acaaggtgag acatttgttg gttgtcggcc ataatggagc
    15661 tgaaggaatt attaccacag gaaactttac taattatttg aagcaaaacg tggatatgga
    15721 tgaagtgaac accgctatca tacagagcct gttggaagaa aaaaaagaag aaggaatgcc
    15781 tccaaactaa actcggccca taactaaaag aaagcgacaa cttacttagc agatgtcaat
    15841 ccggagaaaa gatttacttt ttcagagcaa aaaaatttaa ctagaagttc gaggaagaac
    15901 aagaaaagat ggtcgttgaa ggaaataaaa acagaaatcg aaataaacgc atcagctgaa
    15961 aaggtttggc aagtgcttac ggattttgcc catttccctg actggaatcc gtttatccgt
    16021 gaaattattg gaactgtcgt ggagggatca aagctggaga tacgtatctc aacctctagt
    16081 gggaaaaata ggacgtatag gccgacaata acaaagattg aaccaaatca tgaactgagg
    16141 tggtatggca aaggcctgct acctgggttg ttgaacggtg agcgtatatt tacaatagag
    16201 cagctcgaac aaaatcatgt acgattgatt catcgagaaa tcttcagtgg tcttggcacc
    16261 ttccttggtg gcgaccgtat ggataaggat attcgtcata ccttcgaaga aatgaactat
    16321 gcattgaaaa aaaagattga acttggcggt cgctgagatt cagagctgca gaatcgtttt
    16381 cacactctta acctccacta ggtcttacct cgtacgattt ttatcgaaac ttttaacaag
    16441 ctcacgtggc agccgtgtat cggcgaagtg gacatgcata tatgacgcaa ggcagttatg
    16501 gactttaaaa ccatccttct tgccgtcaac gccattccct ctagtcattg tgtaggcgaa
    16561 ttttgcatcg cctgcgatat ttctaatcct tgagtaatgg aattcatgtc ctcggatatt
    16621 agacacgttg ctaaatgggg atgtattgca gtcagcctta gtataattcn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnngcct tagtataatt caaagttaac ttttgagcca
    16801 taaaagtatc cgcatcaatg agaccgacca tcttcttctt tttccatttt ttattgcccc
    16861 gacggcccaa ttcgtactcg ccaattgatc gggttaggta cattaacccg ccgcattcgg
    16921 cataaattgg agtaccgttt tcagctgcct ttaacatcga cattttcatg gaatggtttt
    16981 tactcagttt ttctgacaaa acttctggga atccacctcc aagtataata ccagatatat
    17041 cctcaggtaa agtgcaatca tgaacaggac tgaaaaataa taggtttacc cgttgtttcc
    17101 tcagagcatc aaggttgtca gcataataaa aattgaacga ttcgtccaac gcaacagcta
    17161 ccgttaatct actggttgac aattgcgctg tagattgttt attacgcacc tcacttggca
    17221 cgttccacac atctcttatt ttgtcaaggt caatctgttc agacgcatat tttgccgagc
    17281 gaattatcgc tctctttttt ttgtcttcaa gctctgcggt agggatcagg cctaagtgac
    17341 gctcttccat cttgattttc ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctttcctact
    17581 ccgctggtta cccctttgat ttttcccgca aaagcatcgc taataaaagt cgcatgtttt
    17641 tgacctgcga cattatttaa aataattcct gctatcttta acgatttact aaaacgtatg
    17701 aaacccaaag ctatcgcagc gatggatctc gctgctttcg ctgcgtcaat cactaatatg
    17761 atggatgcat ccagaagtct ggcaacatga gccgtactac cgaaatcatc tttgcccgat
    17821 actccgtcaa aaagccccat aactccttcg attactccga agtcagcatc aaaacagcag
    17881 ctattgaaac atcgtagtac tccgctcttg cgcatcatcc atatatccag gtttcgagat
    17941 tgcctaccgg tcacataagt atgataactg ggatcgataa agtcaggacc aactttgaaa
    18001 ggttgaacta cgaagccctt cctcttaagg gcatacataa ttgctgctgc aacggtactc
    18061 tttcctactc cgctggttac cccagcaatt actatttttc ttacgctcaa ttgtttgact
    18121 gactattgag tgcataatat attgaattta ttttaagcta ctggatcccg gcctcgacta
    18181 gggcattatt gaaatgagaa ttaggttttt atcaaattag gactgctaac atttttatga
    18241 ttagtcttaa ttctcctcaa gtccctgtta taagctaagc cgaacctgtg agcttcatct
    18301 ctgatatact ggattacttt tagagcggga ctatttctag aaagaaggac aggatgggtc
    18361 gagtctggag aatagatttc ttcattttct tttgcaagcg atatacacgg tacctctaat
    18421 ccagctttat gaatggcatc tattgcacaa tgtagttgtc cttttcctcc gtcaatcact
    18481 atcaggtcag gtagaagttg agcgcgacca cgaggatagt tttcggtttg tattggtgaa
    18541 tatctccttg tcactatctc ttgtatcatt gcgaaatcat tctggttgaa cgtcaatcta
    18601 attttgaact tcctgtagcc atctttatac ggtttcccat caacaaaacg cgtacaagct
    18661 ccgacagcgt atgtatttcc gaagttggaa atatcaaagc agtcgattac tgaagggata
    18721 attggcaggt gaaggatatt tttcaattct actatccctg gagcgtggcc attttcaacg
    18781 tgtaatttaa ggttgcgtat aaccaaatcc attagatctt ttttttcttt tagctcaaag
    18841 tcatctgaaa ctaaaataat gttcacctcg tggtctgtga atcgctcaat cgacgcctct
    18901 aatgtttccc tagatacagg catttcatta acgtaaacaa acctaggaat ttcaagcacc
    18961 gttgaatagt attgtgaaag gaaagtagcg agagaattat ctccaatgag ctcaaaatca
    19021 aattttttcc tgtctgtgat aacgcctttt gttcggcgca aggtcattac atgtgcgact
    19081 gcagtttcga tttcttttat aattcccaca tattcttcgt cgagattacg acgcagaaac
    19141 ttttccattt tttgtttcgc actcagatta tttaatcgac gtagagtatc acgtatctgc
    19201 tgggccttct catagtcttg aatttcagca gctcgcttca tctcagcttc catttcgtta
    19261 acaaacttgt cgatgttgct ttttccacgc aggatactgt ggagcgtgtc aacattcttc
    19321 ttgtattcat ctctggttac gttgttgata catggggcgt cgcaattttt aagaaaatat
    19381 tccaagcaag gcttctttgg taacttgtcg caaattctaa ttttgaataa ttttcttagt
    19441 agtccaacag caagaaattt tgaacttcca cgaacaaaag ggccgtatac cttgcctttt
    19501 ggtccgtaaa tatcaccctt tctattacgt cttgcgacaa gtaatcgagg aaaagtctca
    19561 tctgtgatct tcagatatgt atacctctgc tgatctttta gttctatatt gaatacaggt
    19621 ctgtaccttt tgattaggtt cgattccagt aaaaatgcct cgatctcatt atctgttagg
    19681 atgaagtcta catcatgaat cttgccaacg agtcttgagg ttttccaatt atcattctgg
    19741 tgagggatat tttcgctgat agccgcactt tgttttgaga agtaggagct aacacgcttc
    19801 tgcaggttct tggccttccc tatgtaaatt atactacctg agatatcctt cattatataa
    19861 accccaggcg aggccggaag gcgtttaacc tgtagattaa ttttttctgt agctagcgcg
    19921 atgttcttgc accttttctg ataatcatct gatctattga cagctttggt tttagatatc
    19981 tgcctgtgta gctgtcttct ttcttagcaa catcttcagg cgttcctgct acgattatct
    20041 cgccacctcc atctccacct tcgggtccga gatcaataat ccaatcagca caagctataa
    20101 catctaggtt atgttcaata acaataacgg tattgccaag attaaccaac gtgttcaaga
    20161 cactcaataa tttgctaaca tctgcaaaat gaagaccagt agtgggctcg tcaagtatgt
    20221 atagagtttt tccagtatct ctttttgaaa gttcagctgc aagtttgatc ctttgcgcct
    20281 caccgcccga tagcgtagtg gcaggctggc ctaatctgat gtatcctaga ccgaccttat
    20341 taagagtctg aagttttttt tcaacaacag gaacgttttt gaaaaattct aaggcttcct
    20401 ccacggtcat atccaggact tcagaaattg tttttccttt atattttacc tttagtgtct
    20461 cagaattata tcgtttacct ttgcattcat cacaagttac atagacgtca ggtaggaatt
    20521 gcatctcaat cctccttaca ccccctcctt cgcatacctc gcaacgacct tcagaaacat
    20581 taaaggagaa tctgcctggc atatagccaa gttccctagc atgaggggtc tttgagaaga
    20641 gctccctaat tggtgtaaaa gcattgacgt aagtagcagc attagatctt ggtgttcgtc
    20701 ctattggtga ctggtctatc ccaatcactt tatctacatg ttctaaacca gagatactat
    20761 cgtatttgcc aggtcgttcc ttggaatcat aaaaataatt ggcaagagcc ctgaaaaaga
    20821 tatcatttac cagagttgat ttacccgaac cagaaacgcc ggttacgacc gtcatgcacc
    20881 ctagcggaaa ctgcgcatca atagatttca gattattctc gcgagcacca tgcactgcta
    20941 tccaattaag gggagtcctc ctattttgtg caggatggga cacaagcata tcgccacgca
    21001 aataagcacc agtgacagaa tctttggtgt ctagaatttt ttctaatgta ccacttgcaa
    21061 caatctgccc cccgtgaaca ccggcacccg ggcctatgtc aacgatccaa tccgaacagc
    21121 gggtaacttc ttcgtcgtgt tcgacaacta gcagcgtgtt gtcgagatcg cgcaatttct
    21181 tgagtgtatc gattagtctt gcattgtctc tctggtgcaa acctatcgta ggctcatcca
    21241 agacataaag gactccagtc agattggaac cgatttgagt ggctaatcta atcctctggg
    21301 actcgccacc ggataaggaa gctgtcattc ggttcaaagt cagataagtc aaacctacgt
    21361 tgcgcaggaa gtcaagtctt gaaattattt cttttagtat gattttggct atgtgtctct
    21421 ctgtttctga aagtgttatt ttgtcaaaaa aattgaaaca ttcatcaatt gaaagatcgc
    21481 aaatatcgat tattgatttc tgctgaatct tcacagaaag aacttcgtct cgtaatcgtt
    21541 ttccatgaca tttttcgcaa ggctgctcac gcatgaattg cataagttcc tcccgttttg
    21601 aatcagattc agtctccttg taagtgcgtt ccaagttggg aatgacacct tcaaagcggc
    21661 cacgatattc ccatctcgag tcagaatttc ttgattcgta cctgtattga atattttcat
    21721 cagttccata gattataatt ttgagttgct tgtctgtcat atctgaaata ggagtgcgga
    21781 ggtcgaagcc gaaccgcttg cctacatctc taagcattgc agaccgaaaa gttgcaaaat
    21841 gccctgacca cggtttgact gctccttcca agatgctttt tgatttgtcc ggaataatta
    21901 gttcaggatc gaattctatt ttaattccca ggccgttgca ttctttgcat gcaccaaatg
    21961 gagaattaaa ggaaaaactc cgcggttcta aatcaccaag gctgatgccg cagcgggcac
    22021 atgcattgcg ttgagaatac aacgtatctt cgctatcagt ggaagcaact acgacgccat
    22081 tccccacttt caatgctgac tggactgact caaacaatct acttttatcg tcatcagttg
    22141 gtatcaatct gtcaataaca acttcaatgg tgtgcttctt ttgcttgtcc agtctaggat
    22201 acgataattt tcgatggcca ctagaatcgt agctgctttc atcatctagg tttatcagtt
    22261 cgccatctaa tctcacccgc gcgtatccat tgtgtttcag ttcatcaaat aatttctcgt
    22321 aggtcccctt tttggattgt attactggtg caagtatgat tgtcttcttc tgctctgcgg
    22381 ttctgagtat tgaatctgtt atcgaatccg cagattggct tgtgattttt tgcctgcaat
    22441 gaggacaatg tggtatgcct attctggagt atagaagtct aaggtaatca tagatttcag
    22501 taacggttcc taccgtcgaa cgaggatttt tactcgtggt cctttgctga attgagattg
    22561 ctggtgacaa accgtcgatt gagtcaacat ccggcttatc cattagctcc aaaaattgtc
    22621 tggcataggc tgatagagac tctacatacc tcctttggcc ttccgcataa atagtatcga
    22681 atgcaagagt cgatttgccc gagcctgata atccagttat gacaaccagc ttgttctttg
    22741 ggatctctaa gctaatgttt ttcaagttgt gctctcttgc gccgcgaatg ataattttat
    22801 cgtgcaaact ttatttattc aacctctgat aaaattggtt ctcctaaagc gctccttatc
    22861 tttgccaact tgtccctaaa ttttatcgcg ttctcaaaat ccaaatcctc ggcatacttc
    22921 ttcattgtga actcagtttc aagcgcgact cttactagat ctaacctcgt catcgatttc
    22981 gtattaagtt gtacatccag atactcgtct ggcatctctc ctgctctgtc tggaattgcc
    23041 ttgataattg attgaggagt tatttcgttc tttatgttgt agtctatctg cttagttcgt
    23101 ctcctttctg tttcattgat cgagttcctg atagagtccg ttatggtatc agcatacata
    23161 atgaccattc catcaatatt tctggctgcg cgcccgaagg tttggataag gctagtatta
    23221 ttacgcaaaa atccttcttt atctgcatcc aagattgcta caaatgatac ttcaggaata
    23281 tctaacccct ctctgagcag gtttatgcct accaaaacat caaattcgcc taagcgaagc
    23341 tgtcttatga gttccgtcct ttgcaatcca tctatttctg agtgcatata acgtacttta
    23401 acatgattct ttgcaagaaa ctctgctaga tcctcggcca tcctttttgt caacgtcgta
    23461 acaagcgttc tctgattctt agcaacccta atagagatct catggatcag atcttgtatc
    23521 tgatcctttg taggtctgat ttcaagtttg ggatccacta acccagtagg ccttaccagc
    23581 tgttcaactg tttgccagct tgacttgatt tcatattcgg atggagtagc ggagacaaaa
    23641 atgacatttt tcatatactt ctgaaattct tcaaacctta acgggcgatt atcgaaagcg
    23701 ctcggaagtc tgaacccata atctactagc atcttctttc gggtataatc gcctttatac
    23761 attcccctca attgtgggat tgtagcatgc gattcgtcta atacgaccag aaaatcttca
    23821 ccgaaaaaat ctaacaagca atatggcggc tgtccaggtg ccctgccatc taagtggcgt
    23881 gaataatttt cgattccaga gcagtagcct aattcctgta tcatttccaa gtcatactta
    23941 gttcgagaag aaagtctctg cctttccagc tcggttggca atttagcaag ccaactattc
    24001 aattcctgtt ttatagattc aattgcagat aatcttgaat cttctccaat aacatagtgt
    24061 ttcgcaggaa atatcttcac agaagaagta tctcttattt ttcgcatagt tagatgatta
    24121 tggattgcta tcttttcgat ttcatctccg tacagcgaga tacgaattac atcgtccgag
    24181 tagccgggaa tgatgtcaac tacatctccc tttaccctga agctgcccgg agtgagggac
    24241 aagtcatttc gttgataacg ggcctcgata aagttatgaa tcaatatctt tctgtccaag
    24301 cgcatacctt ccgtcaaagt aatcgcagtc tcgtcccatt cagacggcga tccaagagag
    24361 tatatgcatg acaccgttgc gacgataata gtaggctcac cagacatcaa catcgccgtg
    24421 gcctcaagcc tcattttttc aatcttttcg ttaacctctg tatctttttc aatataggtg
    24481 tctgtctggg gaatatagct ctcaggctgg taatagtcat aaaaacttac aaagtagcct
    24541 acattgttat gcggaaaaaa ctccttaaat tcggcatata gctgggccgc gagcgttttg
    24601 ttatgcgaaa ctattagagt gttcttgttt gttccagcta taacattagc aatagtgaag
    24661 gtttttccgc tacccgtaac cccaagtaat gtttggagac ctggccttct tgagactccg
    24721 agggacaact tatcaatagc ttcaggctgg tcgcctcgtg gcgaaaatgc gttttttgtc
    24781 aagacaaatg cgttgctact cttgagcaca gacggcatca atcaacgaaa cgatcatata
    24841 ttgttatagc atcaaaaacg ggactttcag cggggctgga aaaatgcaga tcacgagcat
    24901 ataggaaaac ttttcttcta cagggtggat aatagttatc atgagcaagc ctgattacgc
    24961 aatcaacagg caagagttct tgtcattttt agaaaaagaa gccaaattac gtatcgatgg
    25021 ttttatagaa ggtgcaattg aggtcgctga agaggtgcat catggtgtaa caagagagga
    25081 cgccgtctcg ctattccttg aaacgcatac atggccagtt gcaatagatg tagtaaaaca
    25141 ctatcgatca gtcaaccgaa ctattactag cgtagaggtt gcgtctgcga tactacacga
    25201 tatactagaa gataatgata gaattcttga ttcgcataaa actaacgaat acggttttgg
    25261 ggcctatcta agctataggt ttggcaacag agtccaggat atagccacgc aactgaagat
    25321 tcggccctta gaaaacttca ccggagccaa caacgaggaa cgcgagctaa acaggttccg
    25381 cgaatattgt gcgattctta tatcttctga atatgacgtg aagacaatca aacttgcgga
    25441 cagacttaac aatatgaggt tcattttggg tgtagcacaa atgaacaaaa aggtaatcta
    25501 cgataaaatg aagcggtata tgcgagaggg agaggatttc tatcttgcat acacaatgtt
    25561 gcagccaaaa cttccttgct tttacgcgaa catacgatcc acttatgaaa agcttcgatc
    25621 aatatatttc gagcaaactc tcaccatgcc acagtcacag taaaggaatc aaacgcgtca
    25681 acatcaacaa cgaacatagg cccacccatg taagaaggtc gtgggcgtta cttaatcgat
    25741 gtctagcaag tacgcttagt tattataaag aaattttaga ggttacgaag gtgtgttctg
    25801 aatatctatt cccaaataga tgaagccatt gaatttccac ccaatgcacc atcagtttgt
    25861 tccataaccc atttaagggc attatatgga gatagcagag ttaatggcct caccgctaaa
    25921 tttagcagta atagtgaaac ttgagcctga tttctctgaa ggcagtgtca gctacaactc
    25981 ggacgggacc ttaaatcgag cagagacgaa aagcattctt ggaccacaca gtgctattgc
    26041 ttcagctgcg gctttctatg ccaaagtcaa gtacggtgct gttatctccg tagcaacaat
    26101 gggaccgcca attgccgatt ctgccttaca acaggcgcag ttaatttctg atgcagattc
    26161 cctacacctt tatagtgata ggattttttc tggagctgat actttggcaa ctgcagaggt
    26221 tttgaaaacc gggataaaga aaatggaagg gaccggaaaa atggacattg tattttcagg
    26281 acatcgagct tcagatggcg aaacaggaca gactggtcca cagactgcat ggaagctagg
    26341 ttataccttc ctaggcaatg taatttgtta cgatgtcgat atcgaaaaac gcattttgac
    26401 agcaaagcga ctgataacct tgcaggggac cccagacgta atcgaagaaa tagaagctcc
    26461 cttaccggta ttcataactg tagacccttc ttataggtca gtttttaaca ctgtctcgct
    26521 gcgtctggcc tcggtaaaat atcagaagga agcggagcaa agagcacaaa atactaagaa
    26581 ttatctgaag atttttaatg cccagcagct tgaagttgat ccgaaattcg ttgggttgcc
    26641 aggatcgcca acgattgtat acaaagttga gaggatcccc aaagctaggg caagtaggag
    26701 agctgaggtt atcgatggct cggaccagca gcaactacgc agggttgctg aaaaaatcca
    26761 cgaaattgtc gtacgagtgg tgaatcaata gcgactatgg ctgcgtcgac gacactaccc
    26821 gaccctagaa caatcatgcc aataagggag aactccacta atagtgccaa tgaaagcaaa
    26881 ggaagctacc gacacctcta cgttgtagta gagcacgatg atggtaagcc aattcctgtc
    26941 acgctcgaga tgctgggtga agcgaggcgg ctcatggata tttttaatgc tagatactca
    27001 tcaactgaga aggtagttgc agtcgtatta ggaattaata tcaaaaaatt gtgcgaggaa
    27061 ttggtttact atggcgcaga tgcagtaata tgtgtagaaa atcccaatct acgataccca
    27121 ataaatttga ttgatacaaa ggttatcagt caagttgcta gagatagggt ttgtgtatcg
    27181 aaggtttcgc cggaatatag tgaagaattt aagaaaccca gatacatgtt cttctccgca
    27241 gacaacaccg gcaggcactt gtcagcaaca gttctcgctg aattagaatc agggcttgca
    27301 tcagacatca acaaattggt tattgaagac cttgaaatca agcacgaaca taagacaaaa
    27361 ggtcagcttg cgagatacga aaaaacactg gaaatgtacc ggccagattt ctcaggcttt
    27421 ctctggacca ccatattgtg cctggataat cgaaatcctg ctatacaaag ggaataccat
    27481 cctcaagcat gcagtatcat accaggtgtt tttgcgccct tgcaacgcga tcccactagg
    27541 acaggaatta tagtcgactt tagtccagaa ttgcaccagg cagataaatc tgtcaaggtt
    27601 ctgaatcgtc aagttaccaa gagccctgtc gatttcgata gccacaaggc ggtaataagt
    27661 tttggccgcg gaatcaagga ttcgccagag gataatataa aattaatagg agagttagct
    27721 aacgaactaa atgccgagat cggagtatca ttgcccatct cgaaaaagcc atattctgtg
    27781 cgcgagccag tctcttcgct ctatatgaac tctgatagag ttatcggaac tagtggtcgc
    27841 aaagtagcac cagcaatata tgttgccata ggagttagtg gagcaatgca gcacatcgca
    27901 gggatgaagg aatcgggatt tgttatcgct atcaatgtcg atgcaaattc cccgattaag
    27961 gacgaatgcg atattttcat cagaggcagg atggaggacg tactaccggt cttaatagaa
    28021 gaattgaaaa agcaaaaaca agttatggag gtccacaaat aaaattggaa catttcgacg
    28081 ttgccatagt aggaggcggc acagcaggct ttagtgcact acaacaattg tcaaatctag
    28141 gcaagcaggc aattttgatt gaggctgcga aaacgccggg gtcgaaaaat gtatccgggg
    28201 gaattctcta ttcaaagacg ccgaaaaatg gcagagtata taatgtggag gacgtgttcg
    28261 gacaagaatt tgtgacgcaa gccccagttg aaaggctcat cacaaaatac attcttcacg
    28321 ctacttcaaa tgacaaggtt ttctctatcg atctcacgcc ggcacacgaa tatcagtcaa
    28381 acttcgcata ttctgtctta ttaactaaat taattgcctg gtttgcaaag caagcaggag
    28441 aaaatgcaga aagacaaggc ggtgggatcg taagcggtgt gcatgttcgc ggggtcagtt
    28501 ggaacggcga taagactata gttgaaactg atgaacttga accgttcgaa gtaaaagcaa
    28561 taattgccgc agatggagtt aactcggagc ttgctgaact atccggggca agacccaaat
    28621 tcacacccga ggatctgtat cagggagtta aggtcgtggt aaagttacca gaagaaatcc
    28681 ttcaagaaag gttcaatatc aaacctaatg aaggtgctgc ccatcttttt gcaggcgatg
    28741 ttacgctaga tcatataggc ggcggattct tgtatacgaa ccgtgacacg ctctccttgg
    28801 gagctgtcta tcactacgac tcactaatgg aaaaccccgt cgagccgtat gttcttgtgg
    28861 ataggttact gaagaatcca cttatcagcg aatttgtcaa agacgaggtc gccattaaag
    28921 aagatatcga caagagtctt gccatacagg aacaattaag agctcgtttt gctgtaagta
    28981 agctactaaa gaattgggga gatctccgtg accagtatta ctctcaatta ggaagggcaa
    29041 gaattattga aagcggaaga tacaaatccg aagaggagat caaagccagg cttgatagta
    29101 ttcgtaacga attagtcggt cggtacaaaa caaaattcgt caatgattat gtggagttag
    29161 aatatagcgc gaagctcgtt ccggacggca agcgatgtag aatgaaaaaa ccatatcgca
    29221 agaatattct ttttgtgggg gatgctgccg gtcgcggcat ttttgtcggt cctaggatag
    29281 aagggcttaa tgttggaatc gacgatgccg taagggcggc taacagcatc tcacgatctc
    29341 tagatagaaa caatttcgca gaggaataca tgggtgaatt ttataccaag tccatcgaag
    29401 acagcccata tacacgcgac atgaaggaga tcgataaaga ttatttgaag ctcttcttgg
    29461 atgcagctaa ggctgtaccg gtggatatcg taaaagctag atacggcatt atcttcaaat
    29521 taatgtcaag tggcatgttg cgtggtcttg ctgtcggctt tgcaaatata cttggttatg
    29581 accgtctttt gccgatggtt gaaacccggg agacctacgt gcaggtgcct atagagctcg
    29641 ccgagaaatt aggaactcat acggcctcga cttatagctg cagtatccct acaatagccc
    29701 agcgcattgc aaaattaagg tacaatgacg atactctttc tcatattatg attttggacc
    29761 caaaaagtga ttttatgaga aagatggtcg tgctttgccc cacgaagtgc tatagcatag
    29821 aacaggacga agtgatgctt cagcatgagg gatgtatcga gtgcggtagc tgcgccaaag
    29881 aaactgactg gaagcatccc cggggtgaga aggggattga attcaagtac gggtagacct
    29941 ccacccaggg agcgagaatc agtttacgga tctgtatggt aattattacc gattattacc
    30001 aacatatata tggctgagta tacccctata ttatgattag aaatggtaga caaatcaaaa
    30061 gatgatacgt tcaaggttgc tgaccgagcc agagacgata ctaacacaat aaggtctagt
    30121 gatacacgcg acttctacga cacgattaga gaaaattcta tcaaaatagt agatgaagtg
    30181 gctagggtcc agccacagta ctcgcagtcg atatcaaatc tccaactcga ctacatccaa
    30241 gcgatgaaga acgccataca cacgacgttt tctgcccaaa aacatctgac cgatagtaac
    30301 ccgggcgctt ggaacagcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30541 nnnnnnnnnn nnnnnncgcc tttcacggag caatttgcaa agcagtcaaa cgaaatcacc
    30601 aataacacaa taagagccgt gggtattaac aatcagttaa ccatcaatgc actggatgca
    30661 acaagggaga acctgaaaat ctataatcga acgatcgacg ctgtaacaga gtttaatacc
    30721 aacgctgcaa aagcctggaa ctctttcttc actacacaac agcaacagat gttcagacac
    30781 tgacctacag cttctccttt tttttgatcc atgacctcat ttttaaactt gaatatatcc
    30841 ctgttctgag gagaaaatct aaaaatctca caatgttgct tataatattt atataattga
    30901 attaccatat aatatgtact tctttaaagt atgcaaactt caatcgtctg ttcagtatgt
    30961 aacaagaata atgagaccgt gacagacccg gattcaggcg aactcatatg tggcaattgc
    31021 ggcacggtat tactcgaaaa catcgtggag actggtcctg aatggcggct tttgggtgag
    31081 ggcaaggata ggagtcgaac ggggatgccc acatctctat cacgtcatga tatgggcctg
    31141 gctactataa ttggtaagac gaatagagat gcgagcggcc gcggattgga tgcatcggtg
    31201 aaatctacaa tggaaaggct aagaacatgg gacctccgta ttcaaaccta tagcccttcc
    31261 gataggaacc taagacaggc cttcagtatc ctagaacgag tgaaagataa actgggtttg
    31321 tctgagcctg taatcgaaaa gactgcttac atctacagaa aagcacagga aaggggactt
    31381 gtacgaggta gaactattac atccgtattg tctgctgcga tttatatagc ctgccgcgaa
    31441 atgggaattg ctagaacgct taatgacatt gcatcactca ctaacctccg tcgaaaagaa
    31501 ctcgcgcgaa cgttccgtct gctaatcctg gaacttgatc taatggtacc agttgttgat
    31561 ccgatgaaat gtattgccaa agttgcaaac aaaaccaagt taagcgagag aacaaagcgc
    31621 caggcaatgg acatcatgac aagcgttaca aaaaggggaa tatcggcagg caaagatccg
    31681 atgggcctag caggaacagt cctctatatg tcttctaaga attctggaga aactataacg
    31741 caaatggata tcgcaaacgc tgctggagta acagaggtaa ctatcagaaa caggtttaga
    31801 gatctaaaaa gcaaaataga cctgaattag tttggacgga aattacaaga acccaataga
    31861 aattgacatc gttttgctaa gaggatgttc cccactgact ccttcggatt agcattaaat
    31921 tggcgtgctc tggcgacgaa acgcattgtt gccactacag acaatagcac ggattgcaac
    31981 tatttcgcct gatctgcagc tccagttcca caagaagtcc tagtatcgaa tattatctaa
    32041 ttcttcagct gtgccttttc gattggcgct gtcccttgcc tgaaggttat agtcagcctg
    32101 tcgccaacgt tatacggaca atgcggaata cctcttgttt gatgatccga tgcctctaca
    32161 acacacgttc cttctgcctt ggtgatgact ttaacctcgt cttacatctt ttctgatcaa
    32221 gtccttgata ggaaagccaa acatggcttg aatgccaaaa aatcctcctg cgacaataat
    32281 gctcactaga agggccattt tcttcccaga tagcccggag aatatgggcg tatcagagtt
    32341 cgagtccggc gcgtatgaca caaataacca gacaatagag aatttaaaac ctctgcagtc
    32401 tgttactgct cttcagactt atgctttctc tgcaacctat tgagtatgaa tttgagcctc
    32461 gaatgaccca taatgaacaa atattcatga ttgacattcg gtccgccata attgttacaa
    32521 acgccctctc ttgctttcag caaataggtg ctcttgattc ttattgattg aaaacgggct
    32581 ccgctttctc acaatcatcg gaatgggcta gctgctacca taatactcga agtctgcctg
    32641 taaacagagg atgcgacagc cgggatttga acccgggtta ccagattggc aatctgatgt
    32701 cctagaccaa actggacgac cgtcgcaagg ttatggaaac aagataaatt atgtatatta
    32761 tatagataat gattttattt tcaggtcaac acccacctga taattaacag tccttttcta
    32821 ttaagattgt ataccatttg aatgtacaga taatagttta taagatcaac attccaaaat
    32881 gagtgggcta cagcaccgcc gatattactt attccgcata gataataaac ccaattaaac
    32941 tacttgcgct atgtcagcaa tataagggtc gaatatcctc gtctttcact tgcataatct
    33001 tgatccattt cttctactat tattgtcacc gagataagat cccgtctttg gcactggcta
    33061 gcatcgatta caaaataatc atctaaaata tctgctgctt tcagactttc gttgctgaaa
    33121 ttaaaattga caggagagct tgagctaatt agcactagct gatctttatg gtaatttcct
    33181 attctggcta acctggcctc tcttggtgtc gcccttacgt ttagcttgac ttgtcctaag
    33241 gaatgcatcg tgtagcgtcg catgttattt acgctgactt ggaagttaaa cggatagccg
    33301 gcagtatttt cagccatttt gttaatgcca tcattcataa ctatatcgat cttcttgata
    33361 ttatcagaat atctgtaaat ccattcattc gtcgtttgga cgatattatc gatggtcgct
    33421 tttctcgatt ttgtttcttc ctcgcttaac ttatatctat caacccaatc cttgtagtcc
    33481 ttacttatgt ctacaataaa ttcagagcaa gtgttttgat agtcttcaac gctgcttgca
    33541 cgttcagaac cggtgtcagg tgtggacata agacactctt gtaattatac aataagacgt
    33601 aaaaaatgga tcgagcgacc ggctcgatgg attgttgtta tacatctacg aacattcata
    33661 tatatgtaga accaagtttc atgatcgtaa acgcttctgg caaaatgata acgttttcag
    33721 aattgattga cgaactgaaa tcaaatatta attacaatag ggatgtgctt tacgcaatct
    33781 caaagaaccc caatttatta tacaaaaaaa taactgaact cgcgtccttc accggctccc
    33841 gtcatcaagt agcactccag ttgcattttc cagatccgaa taaaatcaag gatattgatt
    33901 catacggagc cgaaaacatt agtgtcgtca tagataagtt tcgcagaaaa ttcgctgttc
    33961 ctagggagaa tataagacga aaagcaatcg aatccctggg gaacaatatt caaacccaag
    34021 atgcctacat gtacgaagga aaagaaggtt tacgaataat caaagaaaat ggcaggattg
    34081 aaattctccc tggctccatt catttatggt gcaaagtaga tcagaatgtg aaaaactatg
    34141 tagactggtt gatggaaaat atatacagcc ctaacaccgg cgggatttct acttagcatt
    34201 actttcattc tcgataaata aaaggttcaa aactacagcc tttttccgac caattcgtat
    34261 tgatattgca gtcgaccttc aaaatcgtat acctctgctc gaaccggaag aggaatctgg
    34321 tgagtgatga agatcttact cgttttcgaa ccaattgtat aactcaaagt aaaggcatca
    34381 aaggttcctg cggtggtctg caatgtctcg tgattggtaa ctcttatggg aattgaggaa
    34441 aaagccacaa atatcgagtc ccaaggcgcg ccaatcgcca aatatttcgg ctctcttgca
    34501 atatccctta tcgacaaaac agacgattct acaggctcat agtacggctt gaaggatgtg
    34561 tctattgaac caacatttgt taattgctgc ttagccaaca taacggaacc gttcttagct
    34621 actgaatcgt tgtgaatatc gaagttgact ttccaggcac taccagtatc ttggacaaaa
    34681 ttcatagata ccaatgcgtt gatgagagaa cttcgcgagc ctatactagt caagctataa
    34741 ttcagtactg tgccttgctt aatcccttgt cctaccgtcc ataaatcggc ggcagtagaa
    34801 ggatgtaaaa tcgacggccc cgggccagaa aatccacctc ctattgctac tattatgatg
    34861 ctcacgccta ctccaaatac accgagaatc ccaaacatca agagggcctg tcgcttaggg
    34921 gacaattatt ctgaataatg tagatacggc ctatttattc ctagacaaaa acaaaaaaag
    34981 aaagtatgct gttgtatgat gatggtcttt ggtataaaaa tccgcccgct gcggcaatta
    35041 atataacgtt aatgcccgct cactcaatta agcctttttt ctctcttaga gaaaaccgag
    35101 tgactatgta caaaacaatc ctactaaagt atactagata acaaaagtgt ttggattgcg
    35161 tatataccaa aagccatttg gaacttgggc tgaacctact ctacagcaac tttatttccc
    35221 tgatgctggc gcagccacca agttgaaagt agcgctttcg acaaactctc cacttggaac
    35281 gcccccaaca ccatccaccg atatgaccat actaataggt ccaggttttg ggaacgtaat
    35341 tgtttgtatg cctgttccat cagcagcatg ttggtcatgc acatctgcag cggttgtatt
    35401 tccttgggta gcttttacgc tgtaggtgac atcgcttaaa acactttgtt ggctattttt
    35461 aaacaataat ccgaattgcg tgggttttcc agcagttatc gttgaaggtt gccatgcaac
    35521 tatctcaatg aaatacgttc cgcgggctgt ttgtatcgtt atcggaggtg tgggaactag
    35581 attctggcct ttgggaacag gaggctgccc gtggcctgtc gcttcgagat caaatgcata
    35641 ttgagtagga ggagttcctc ctgtagtggc agcgtaggtt tgcgcctgga ttggatatgg
    35701 catagtggga ttaacataaa tattattatc tacaccataa tgccaagtga ttactgtggt
    35761 gtcaaacgtc cccgcaggta cggtaacctt tgcactaccg cctggtgcca cagctgatcc
    35821 tccaattgct gcaattttac cccagtaggg agccgttaat gagagccctg gtttgggaac
    35881 aaaagccgat agccacgcaa gactgttctt gtacccagat acaaaatctc tcatctcagg
    35941 tggaattttg gaagatccca aaggagtgag gtctaggtca cttagatgga gcgttccatt
    36001 gtataccttg ccttggtata cgacggttgc tggagcaatc cagtacttgc cagtactgtt
    36061 gaactgctta aaataaattg tcataaggaa aggctgaccc tgtacagtgt catggtcctg
    36121 gatcttatag gtatagtagg tatctgcttg gaccccgttc cctaagtaat agttgttcgc
    36181 agcgtatgca ttcttgatga agcccatata attcaaagca ttgcctgtta gaacagctga
    36241 tatcaatatc aacgacaaca atatcgattt ggaaaaaata cttgtagtac tctttgccat
    36301 tgtctatcaa agaattagaa taatttagtt gatatatgtg tttgtgctcg agacaccacc
    36361 cattgagacg acatgattgg atttggatat acaaataatt ataggctcag cgttatcgat
    36421 ttagagctat gtgtagctgc ccaaaagttc acttttacga agttgagttt aaattggccg
    36481 gaatgcgggt tattccaacc cacaagaact gcggtgatcc attgagcgag caacagttgg
    36541 caaagtttga aaaagaactg attcaatatt ggggctttgg ggaaaaaagt gtttagagcc
    36601 tatgctgcgt aagtcaatcc gaataagact gaatttattc ttgttttctt aagttgtgac
    36661 gtaagatggt ttaaattcat cccaaaggtc ttttgtactt cgtacgtcaa ctttataaat
    36721 ctctacaata atgctgcttt aggtgatttt gtatgaccaa gatgctaaac gtaaatatag
    36781 atacctccgg agtcgataaa caagaagctg aagaatgggt aaatgaactt gctaatgtct
    36841 atgcagacat ggaaattgca gacataaatg tttccgggaa taagattgca ttcaaggctg
    36901 gcttcggagg catggacgac accgagcccg atgatgtcaa aatgaagata gaagagtatc
    36961 tgacgatgaa cgaagccttc aatgcatcga acgtatccgt gcgctagggt ggatggacat
    37021 ataagctctt ttcttcctct tcttttcttt tctttatttt ctgtccctat ccgcacgtca
    37081 gaacaatgcg aatagtgtat cattattcct tattacagta ctggttactt atcgcgggtt
    37141 tttgatgggt ggtaacattc acgggttaaa cctataaaac gaagtctatc aagaaatttg
    37201 ttttatgtga gccgttcaat ttctcatatt ccaaagacag ccacaataac gcccgaacaa
    37261 tatcctcaat atactgaaag aatctgtgtt tggacagccc ctcaaaatat atggtcttgt
    37321 atatctcgat gtgttctatg gtgttaaaat ttggataaaa attatactaa cacctgattg
    37381 aaataatact gaacgtaatc tccgaacaaa gtctacacgt ttatcatttg aatgcgaaag
    37441 ggctttctta tctaggtccg agaaagcaga ccgagctact agattgaggc tgtcaatttt
    37501 ctgtttatca attgttatat gtataggcat gtctttggct ggatagacta cgactttgaa
    37561 cattcgatat ggatctgttt cttcttgaaa tatagctgac tcctctttaa gccattggat
    37621 aatcttacga ccaacatcaa tcttgctttg ttctgcggta tccgacatta tttccgcttc
    37681 tcctccctat gaataattaa tctatcgctg tcatccaaac caatgcgaag cgtatcacct
    37741 gttttccaac ccaaatctcc aatcatttcc atcggaatcg tgactctgaa gctattgccc
    37801 acttttccta gcttgacttt cggctcaaat ggcatgtaat atcattgtat gcacgaggta
    37861 tatatttaaa tatacgtgat tactatgtag atacatcgat atcacattac gacttacaca
    37921 gaacacgaga aaggccgaca agtaagagga ttagccatag cctttattgg atttgaaaaa
    37981 tatcgcacga ctcctaaagt cgttaccgca gcccttgact tgtatttcaa aggggtatca
    38041 ttgagaaaga tttgtgacca tttaagacaa ttctacggcg taaagatgac tcacgtatca
    38101 gtaataaagt ggctccgaaa atttgtagat gttgtaaagc catacgtaga ttcaatgagt
    38161 cctcctcgac ttagcggatg aatcatgttg acgaaatgat gattcatgcc aaacgtgaac
    38221 aaatggaagt agtccactat caatggcttt ggaatttgat ggatgatact acaagatttt
    38281 ggattagcag tatggtaagt caacgcaggg aagtcgcaga cgcgcgaaga gtatttcagg
    38341 attcaaaaaa caagacaaat catacgaaag caattatcca cgatggatta aagtcgtatg
    38401 atgaagcctt tcaaaaagaa tattttagcc taaaaattcc gcgcgtgtaa aacattcgta
    38461 gcgtctcggt tagacatgaa ggattaaatt caaaagtcga gcgtttacat gggacgataa
    38521 gagaacggga gaaagtcatg cggggaatgc aaacacaaga aacagcacaa aaagtcgttg
    38581 atgcaatgcg aataaattac aattacatcc gagaacactc aacaataaag aaaacaccgg
    38641 ctgaaaaagc aggaataaaa ttgaatttag gagaaaacaa tattgagaat ttgattagat
    38701 tagcatctac taaagagaaa aagctatagc tttgcggcga tatttgttag ctgatgatta
    38761 gcaacaaaca tctgatcaca attataaccg tcctgtatgt cccaattatt gtattcatgt
    38821 tcgttgatga aggggtattt tggggactaa tcggtataac aggagcaatt ttgagtctag
    38881 ggattccata tttggttatc tttaaaaagc gagcaacaat aatgcaaata ttgatggatt
    38941 tggcaattgg ccaaatttat gagaaaattg ctgttttaga agggtatgta agcgaagtgc
    39001 ccacttggaa ggaaaaagga attgatccag caaagagaac gaacagagta tgttatgata
    39061 tcaagtctat gtggagaatt aaaggatgcc gccagaacag aaatcagaat tatctggggt
    39121 tattcgccac ctgatagaag caatggagaa agaatacaaa gacgaggctg ggagagtaac
    39181 agacacttca gcacctttga agtaaaaccg cgaaagtaac catttagaag agataaagct
    39241 gaggacatca ttttttgatt tagcgtgttt actctaaatg aattaaaaca aatcgttgaa
    39301 cagcatatga agaatgagct cggcgttatg gagctcactt tgagcttaga gaataaagat
    39361 gtagacgaat tcaccagatg gtatagtaat cacccaatca gcaggggcat acgagaagta
    39421 caggaaattg tcagaagagg accaaataag ccaaatcaaa atgaaatttt tgagcacgat
    39481 agacactcag gcaagaaaaa acgtatttga tgaattggcg aaacatggta ataaggg
//
LOCUS       DAOR01000259            6448 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_65550, whole genome shotgun sequence.
ACCESSION   DAOR01000259 DAOR01000000
VERSION     DAOR01000259.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_65550"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1919 a         1221 c         1233 g         2075 t
ORIGIN      
        1 ctgtgagttg agctacaaac tatgtgggac catatccttg atatgtgcta gaaatttggt
       61 tttccctttc tattttaccg tccttaccgt acggttacga gttgcttaga aagggtaatg
      121 ttaaaaggta gagatgatat ccaagaacca ataattatgg ccttgatctc tgattttcat
      181 gtattttcgg tactcgatat tttcctctaa cttttcacga tgatttaaat tgattagttg
      241 ttgtacttac acagatatgc tacacttctg attacctaaa tgagttacac gttggtgctt
      301 ccttgtcaca gtattagcac cacctaacgt tcctagaaac gtctctgata gagtcagacc
      361 atttattcct ccatgttctc taggctcatt atatgccttg acaaacatat catatcttga
      421 tttcccatca tctgtgcttg gtatatcttc aagttgctag aaactcattt ctactacttg
      481 gtgtatgctt ctacctttcc ttgtagttgc ggataggaat taggaattag tttaacatct
      541 aatatgatcg tgtttgagaa aaaacctgaa taatcgatgt aaactgcatt ccattatcat
      601 acattatctt cttgggcttt cagttgatca ttaccatttc tccagtacat gcagagtatc
      661 gatagttctc tttctctcag accatctact tgctacctct ctgtagttac ctgttcttcc
      721 atttgtagta tatcatccta agtataacat atttcttaca aaatactgat tactgtgtca
      781 ctgtgtcacc agtccattca tgttcataaa ttaagttgaa ttttctttcg attctatagg
      841 atatacactt catatcaaca gcgtaaccta agtgtgtaag tacaacagtt agtaaattac
      901 taaatcgcgt aacgtctagt gagttcctat attaatgcct gcaactattg ccataagtct
      961 tattacaatg tatggttata tcttcacaag ctacaacatc ctgtccttct aacccaaact
     1021 aatatgacta aggtttgtat ttttagtgtt taatgccatt tcaatatcta tatttatcca
     1081 actattgtag ttaacagtaa ctctatcaca cgaatatcgc ttttagatgg aatatgtggg
     1141 gtgatgtcgc tgaaaccctt tttgaatttg aatggatttg gtagggtttt tgccacttgg
     1201 tcggtatctt tgttggtcta gacttgcaat ttcgtatcca agtcaggtag caaacttgaa
     1261 ttttgcatgg acggttatag tcgacggcga actttgacaa atcacgttct gctttcttaa
     1321 agccgtcgtt cgccatcaac gaacctaggt cctgcgacat tgctcttggc tttgttttca
     1381 gaggtgtctt gggcctacct cagaaggctt aagtaaaagc tatcttcagc ttaagatata
     1441 tgaaaacaag agaaacaacg attattattg gagttttatt tggaactatg cttgttatgt
     1501 caggagttgg attaacgaac actattctcg ttaatgaaac atttgccgca gcagcaccag
     1561 gaaatatgac aaatgcaact aatacaatga atcctcaagc tgttgttcaa aaaggagcaa
     1621 cttcttcaag taatgatgta aaaacaacaa taactaacaa caatattact ttgggaaatc
     1681 cattctatat agaatatgat aaaattacaa gtacggtacc aggtattcct ttaaatggcg
     1741 gaggacatgc tactggagta acattctcag gtaatgggat tgtaaaaggt ataggcttca
     1801 cagatactgg cagagctctg atcatcccaa tatctaaaac aattgtaggt atttttggag
     1861 ggttggctat taaggctaat gatggcggcg gtaatgctaa tgggaatgcc acgcttagtt
     1921 ttagagaggt tgtacacatg actcctgctg gaacaatgca aggaagtgga gcagctatct
     1981 ttaatgcaaa tgcaacacga aatctgtctt ttcttagcaa tacagtagcg atgtttacta
     2041 atactggtaa caaagatggt accgatacaa ttaaggcttg ggaatggaag tatagataag
     2101 acaaaaagat actcctctaa tcctttattt atctattaag ttggggaaaa taatacctta
     2161 taacacaaat gcaatatttt ctcattgata aagattctag agtatgggta tagctgctcc
     2221 tagtgctgag aaagataagt taaatgaccc cctcattata cttaattacc acgatacctt
     2281 tgctatctta tttttgtcga ccggctgttc taggtttgat atgacagtta gaatattttg
     2341 aagtgttgac ctcatttctt tatactctag tttcgtaact tgcatttcat tgtcctcttc
     2401 ctgtcttaat ttttgaatct gatccccttc ttcccttaat tctgacatct gcctttgtag
     2461 cctgtgagaa tcgtgtcgtc gatcgttaga tggtctatcg cggaaataaa acctcgcatg
     2521 ttatcgttac cttctagtaa gtccacatca attggtttaa agtaagaatc tttaacatcc
     2581 tttatacggt gaccatctag tagctcaata taaagaggac ttagaccggc tttcgtcata
     2641 gtcgcttcac tcacgctata tggaaaaatg atgagagaag gcatcacagt ggcatttctt
     2701 aacacctcca ctttctaaag atgaagcacc tctgctgcac tagttgctat gccgccaatt
     2761 tgatgcatta tgcccagcaa gtctccacaa ctccacatct ctactacctt accctgtgag
     2821 acgcgataaa tgtagttctc tgtccaagtg acctgcttac ctgtgggcgg tacaccttga
     2881 aaagtacccg tgtgtgtggc tcgtacgtta ctccgttcta cgacgagatc gccatcgaca
     2941 attatgaact ctagtgtcac atgcaggaaa ggccttgaag atcatgttgg caatcgcttt
     3001 aaagccttct ttgccagctg gaagtttagg gtcgctaaaa tggtggacaa aattagatgc
     3061 taacagattg tccaagggtg ccatgctcgc gcctccgttt ttgaattcat tcacaaacct
     3121 gtgcacgacg gttttatgtt cttgtgtata tacaggggct catattttaa gatttcaata
     3181 atgctttaaa tattaaagta ttataatagg ttgtccttat ttgttcgtgg agacagacaa
     3241 ggtgcgtgct ttccttttga gcagcagtag gatttgtctt ctactatcgt tattataaaa
     3301 caatggtgcg ttctgtcctt attgcgagat ggcattacga gtcaccaact aaaaaaaaga
     3361 taaagaaagg atgaggaaca ggaagagaag aacaagatag aataatcagg atcgtaaaag
     3421 gggataagaa gtacccagtc aatcaagaca agtcaaaatg aaatgaatat atctaaatta
     3481 cctcacagcc acaggtcaaa ttctagcaat tatttgcgat agagtctgaa tctttacatt
     3541 gataattatt ctaaaaactt gtcaactact gcggatctta aattcggtaa tatcaggatg
     3601 acttgacaaa gataggttga cacccaataa cattgcggcg aagtatatgt atccggcagc
     3661 aactcctttg ggagatttgc catctgcaag aagatgattg cttgatttgt gagctatttc
     3721 aatagctaac tcgggaatca atccttgtgg aggtggttgt tgaaatctag gttttgctag
     3781 attccaactg tgcaatcgca ctatcggaga tactggtact ctttggtatc agaataataa
     3841 aataattctt atcagcgcgt tcaaccgaag tgactgattt gtatttttta gtagaaatgt
     3901 caaactccac catccctgcg tcgatgctgc ctttagtata gaccatcgtc aacacttctc
     3961 ccgacagcgg acttaaaggt actaacgaaa acacataacc cttgtatgag cttacaggta
     4021 gaagctgctt catataggga gcaactgata caagatatag aaaaacatcc tctatggtcg
     4081 aaaactgttc aaattcaaat ttcacaagct tgctcggacg tttccgtgat ataagtatag
     4141 ggttttaatg ggggaatcaa ggcaatctga aatagaatgc ttggaattag gcatgctcat
     4201 actcaacagc acgggtatgc gttgtaccag aatcaatcaa agatataaat gagaactttg
     4261 gtacgtttat taattgttat acgatctgat ctcaattatg tcttatattc tgatagtttt
     4321 cattacgcat caagtcattt gaggcttgct ctagcctctg tagtggggga gagctatcga
     4381 attattttga ggcaacgttt tttccttaat tcctgggttc tgtttctgat ggttacttct
     4441 gttactcctg cagctgccgc aagatcactc tgagttctat gttcattact atctctatta
     4501 ttcatggacg ataggtaaag catcgatgct gccagcccca tgggatcttt tcctgcagct
     4561 aactcattgt cattaatctg ctttacgttt tgttatctcg cttagattag ccttatttgc
     4621 aactcttccg atgcacttta taggatcttg tattgggatt ttaagctcaa gtttcgtgca
     4681 agatctttac gtgctaatta gagttggcga caaataactt ttgttccttg ataccaaatg
     4741 atggacattt aagaacacct gttgctgttc tttctgtttt gctatcaacg acatcaggca
     4801 caggaagatt gaccgcagca atcatctact atctcctcca cgcaacgcaa cttcaacaat
     4861 ggaagttatg actattattt tcctgttcat ggtagttaaa gtctcgacta ttaaattgat
     4921 ttttccagat tatagccaaa tttagtccaa atcttgacac gataaatgac actgtgtcta
     4981 atgcactacc acgaccgcaa cttgaatttg atttaagagg aagaaggagt tggctaccat
     5041 gacaggatta gtggatgatt tgggtataat ttgggtattt tgttaaatat tcgagatgaa
     5101 aaaacactat atgttagcta gtataagatc atcaaaacaa ctaaaggatt tggcatgtag
     5161 accgaaggtc atgatatttg gaggtatggc gacaatggca gccggaatgg ttcttactat
     5221 agttgccctt ctatcgcata caacatgata tgtttgaagt gtatgtaatt tctagaatag
     5281 ttcggcttgg tcaaagcgta tgatgaggaa tcgatgtgaa tctgatgccg atctatctcc
     5341 acaggaaatg gaagctattc acagtatata aaaacagaga tctgaaatgt catctggtca
     5401 ttacaataaa aacagaatcg tccttaattt aggaatttta gacaggcatg gttaaacttc
     5461 tcttctctcc taccagaacc atgagaagca tatacgagaa gacgctttgt tgcgattctt
     5521 aacttagcta caaatttcgt tatatcttct gtaaagtaaa actctggaga tgctctactc
     5581 gttttggaga aacacatgtt acattactta gctcctcctt tccatatgcg ctgaatatat
     5641 aactccaaaa actatgatta cgcgacttag catggtagct tcggctcgaa aagcttgcat
     5701 tccgattcgc ttgggttcct tctcggaggc agctaaagcc tcatagaagc agcattcagt
     5761 tgtagagatt agcgatgaag aattttagat taattagatt attattcgaa ttatacgctt
     5821 gagcaagaga gagcatccga tcaaatatgt ttctgtctga tttatttaat aagtttcgag
     5881 agcatcaaat gagaatttct gtaagaatgt ggcatgatgg aatatcaata gaacaatgat
     5941 ataagaatca taaatgatat gggtgattta gaaaccagaa taaaaagagt cgctgactcc
     6001 ttgaattttt attaatacta acttcaccat agcagcacgg gagaacggaa tactcataat
     6061 gggtatgaat ccctgacgat aattataggc tcctttcatg aggggtctag gaagaaaatt
     6121 tcatagctga agagcaaaac atagggtgtg aatcttacat aagttatcat taatccccat
     6181 ctaattcact aaatagttat gatattctag atattcaaag tgctagtaag catatgaaaa
     6241 ggcctcagca ctagccttac agaccgctgg tagaaccagc tgatcaccag gaaactcgct
     6301 acagtctcga tatattttac ttatttacat ttgagttctc cttaaaatct gggcgctcgc
     6361 atcgaccatc gacataaatc caatatttct tatgggaatg agttgccata ggattgaggt
     6421 tcgttcatca ttacagggcc catcatca
//
LOCUS       DAOR01000260            2849 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_138562, whole genome shotgun sequence.
ACCESSION   DAOR01000260 DAOR01000000
VERSION     DAOR01000260.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_138562"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          933 a          501 c          506 g          909 t
ORIGIN      
        1 tgattatggg gcgagaagtt gttgttgcca ttactaacgg gaaactagac tttggtccat
       61 gggagcagat attttacggg gaatttgacg gccgacggga taaaagggta ctagtgaagg
      121 taattggcga ataaagttag aaaactacgc atcgtcggcg gtcactaagg ctggatagag
      181 agtattaata gtcttataac aaacagcgga tcctaaatcc tccacctaca aaatcattaa
      241 tcactgaatc attttccatt agtaaatccc tctcgttaaa aacttttcag aacatgcaaa
      301 ataagaatgc atttaatacc ttgaattgaa aagagagata agatgtcaga agatagtgct
      361 tctagtgacg aacacgtaat tgtttgtagt gaatgtggaa gagaattgat agttaaggac
      421 attgttaaca acggagtggc ctttctaagt catctgaaga tcgaccatgg tataacgtat
      481 actccgcaac gcatgactga agtaaaaact gaaacaaaaa gaagtactga gaagctaata
      541 tacaaaacag acaatcacta atacgatgga taaaagccca ctcaaaaaag gtataaaata
      601 tacattgatc cccagtgaac gaaattgact atagctacct tgagagcacg aatttctcgt
      661 taatatttag tccacgctac atctgatgtc ttgtctacat ttttcaaccg tctcaatttg
      721 ttaatatcag attattactt gtcttcatgt tgttcatttt gtcaatttca tgtcctgtat
      781 tgaaaatatt aaaaatagaa gaagttcaga gacttctact atgctagtta aaataatttc
      841 ttagtagaca gcttgtaaca tacgatcaac ctcttcttct ggaacatcaa aaaaatcaaa
      901 tgcagatctc ataagtttgc tatttgtacc atcatatctt ttttggttta ttcttgcctt
      961 gacaagtgtt tctgaaatct gtgttaacaa tacctgtgcg aagtgattcc actcttgcaa
     1021 gtctttgtca gatcttagag atgccaatat aggattcaat tttgcaaatg atgcaattat
     1081 aagtactctg tacttctgca aatctttttt atatggatta ctatcaggta tagaccgaat
     1141 ttcagagcac acgatgtgtt ggtattcgtc caaattcttt gcaattgtag aggtgctgcc
     1201 aatacttgcc actctgtttc tctggaacct aacattaact gctacacctt caaaaagccc
     1261 ctctatttta tctatgacca ggtcttgcga tgtgatgccg tccttggata ataatttatg
     1321 gcattttata tcattaataa tgtacctatt aataattcgt gaataatgac tattgaaagt
     1381 aataataata ataaattctt actccccata tatacatagc tcccgtgtct gaatgggcta
     1441 tgtagctcca atcggttaaa gaagacgtga taatatattt cattgcctca tcaaaatttg
     1501 catccctgcg tactactatt cctatatact attcctatac tacttgtaaa acagtggaag
     1561 aaggaacgca gacctgggta atggtccctc ttcctagagg cgaaccatta aaccccctca
     1621 acaaccaact caaattccac tattcgataa ttgaaacgaa aatattctgt atacgtatct
     1681 tgttctttct gttctgttac aagaataata acatatcatg ttccacaaac attaagaaag
     1741 atgtgatata ttacacaaga aggtgaagga tagccttata atataacatg agacaatata
     1801 gtatagccga gaaatgacta taaattcaaa ttctagaagt tcaagttcag caagctttac
     1861 tggaagtcgg attggactac cgaaagagga taaagcaggc ttagaagata attttcatga
     1921 gtttattaat aagagaaaat tccgatttcg tgggaaatat aatcttaata ttctggactt
     1981 agagcatgag attagtaaat ggaataaaac gtacacttcc tttctatatc atgccagcat
     2041 taatcctgaa ataatatatg aaagggcgtc tgaaatatat gaggaaggca gattattcca
     2101 gaagcaaata agagatattg cagaaaagat agtccatgat gaagaattaa agttgttaaa
     2161 gtaaagtcaa ttactagata ataagaaagt tagttgaatt attttctatt tgtatcacac
     2221 ttgacctatt attagattgg tagtgttgcg gctattttga tcttcttctg gtgtccgact
     2281 aaagattgtc ttaagcacct taacctatcc attatggccg tatcagactg gtaaatgtct
     2341 cgaaaaagcg gactgtattt cgacattcgc tctgctcact atctcgtaac agtttttata
     2401 ataacgctta tcttaattgc ctacataagt caagcacgaa cgggcgagta gaagattgta
     2461 aatatccatt tgttggagtt aattctcatc atattatatt acttttcttg atattcctac
     2521 agtgatattc tgtgcatgct tacattgaca gatacaattt ccaagcccat gtactgaaaa
     2581 cttgtgatga cacctaaggt ggttgcaatt ggttctgctt tttcagttgt tggataataa
     2641 attagtgggt gtagtacctt ccgtttttct tcgacacttg attgggaata gttcactaaa
     2701 aatggaggaa aagaggcgct actcaacctg gctattatat gatccacttt tctcggctag
     2761 ttctttcttc caatggcagc tccagcagaa gccacatttt aggcatataa attcagtcct
     2821 atttgtacaa gattcgcatt ttgtatacc
//
LOCUS       DAOR01000261            5938 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_12494, whole genome shotgun sequence.
ACCESSION   DAOR01000261 DAOR01000000
VERSION     DAOR01000261.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_12494"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1900 a         1127 c         1013 g         1898 t
ORIGIN      
        1 tacatgtctt atctgtattc ctgcaattag aaatactttg actaaattta gattttcttt
       61 atctatattg ataatccatt tgtaggtaaa cataatttgt atagccccta aagtcgttta
      121 atcgccattc gttatcgtgg ttgcctctgg attttcttta atatcatctt ttattagaag
      181 cactccccag aagtcgtttt tgtattcaat gaacttgtat ttgctcctat agtaatatct
      241 gtggttacgg tatcattatt gacacttcat acagacaagt tcgtattcga tttggatcgt
      301 tatttgcaac agaaaattgt tatcctataa gatatctgaa tgatgctcag cgcaagccta
      361 cttaatattc tcttagatca gagacaatgt tgaatgtagg gtaagtacgg tctacggtct
      421 agttggagga ttgacataat gcgttcagaa caaccggatt aagaagcaat aacaacaaaa
      481 acagtattag tcagcaaaat aataatgcgg tccttcgtgc ctattcatta agaatgttaa
      541 tatattaaat gacttcagat aagcatgcgt atttgagaga taataggaaa cttgggattg
      601 tgtttagtat tggcatatgt gctatgatac tgagtggaat tattatcctt gtgcatgtta
      661 gtagtaatgg tgacgtggct tttgcacagg tacccttttc tctgaagagt acaacgacaa
      721 taaatccagt tgtgaataca agcaataaca ataacaccag ctccattaat gctgcaataa
      781 ataagttgaa ttttactagt gcaactgggt ctgttgcaag tttacagaat gacgcaagcg
      841 gcaaaccagc atggatagtt tccggccaat ggcagatgtt ggtcttcaaa ccaagacttg
      901 aagaaagtca attgaaacca gccacagtta tcttcaatac aatattcgac atggtgcgcc
      961 cagatggagc agcattacat acacacacaa tatcatctac ctttaatcta acaggcatca
     1021 gtaattcagt gaataataat agtcacttat catcgactac aatcaacggt actgctagac
     1081 tagtcacgat agggttgcca aacatgatag gtatctcaaa cataaatgtg ccagtaagcg
     1141 tcaagataat agatcgtagt gcattcagtt tatggattga tccaagcaag attggtaatc
     1201 attttgggaa tacacctata tatggaattg taacgacaaa atctggctaa tatcctcgat
     1261 actttttcca ctacacgtag ttgagccttt aattttgttg tatgttgatc atcttttata
     1321 atagatattt atataaagca acaattgagt attagcaaca gcagcattga aaccagaaaa
     1381 aaataataac acattcaaaa ctattttgat agcctcatct atgattgcat taacaacaat
     1441 tatgattttc atcgcatcga ctttctcatt gcttacaatg ctagtaacta ctggtggtgg
     1501 aaatgatctt cctattgctg cagcacaaca gcaaccgatg cctatgacaa tgaatcaaac
     1561 tagtattggg gcaatgtcaa cagccatact tggtccatca acaaataaga cattttacgt
     1621 attttcatcc tcacatcaag acattaacca aacactatta caaattccac cagactcctt
     1681 ctccccatca gagatagcag tcaacaaagg agacacagtt aatgtaattt tctacaatct
     1741 tgagaaaccg ccaaaaggcg atagacactc gttcactatt ggggcaccat ataacattga
     1801 caaggatcta ggtccaggac aacattcagt aatcactttt aaagcaactc aagatggcgc
     1861 ataccaattt tactgtaagt atcatctgcc aacaatgaga ggggaattaa tagtactacc
     1921 ataatacact gaattaaaga ataattctct tttcattatt tgtgtgtttc ccatattaaa
     1981 cctgctacta gaggcaccta ttttgattcg atatattgct atatgtcaaa ccgatttcat
     2041 cacatgtctc gattaacaat tagaatgttt gatattcatt gttatacgaa tatcacatct
     2101 ttagataaga tattcatttt ctataaagaa tgactaattt agacgaattg ctagcgtttg
     2161 gtggcgtacc cacgctacca caacccaatg gataccacaa aaggtgtggt catattctgg
     2221 tggcgattgg accgtaatga ttggggctaa ggccaataga ggggtatttg ttgaaacagc
     2281 gaaagcagat ttcaatcggt tatttagagc cctagtgtaa gtaaggactt ttggatagga
     2341 tgatgaatga gtaccatgtt ttcgctactt tacaagccgg cgtggccttg tcattcgttt
     2401 atgataaagt taaattacaa gttttgctgt ctgtgatgca gcaatattaa ctggttccca
     2461 cacaggtgaa gctggtggaa cataacatgc atcatagctt gcaagatctc ttatgtctgc
     2521 tttaagtaag agagctagtg caattaaatc aattctctct tttacgccct cacctccgac
     2581 aatttgtgaa cctaatacta ttccactctt tctatcagca acaattttta tccaaatcgg
     2641 tttattgcca ggatagtaac cagcccttgt tatactttct atcacctctt caacaggatc
     2701 aaaaccttct cttaatgctt cttcgctagt taatccggtt ttgccaataa ataaatcaaa
     2761 taccttagtt atagcacttc ctgctattcc tctaaacatt gcatttccac cagctgcgtt
     2821 ttctcctgca actcttcctt gtttattagc agttgtaccc aagggaagat acatgtgttt
     2881 atttgtaatg tagtttctag cagttgcaca atcaccggcc gcaaatatat ccggtatatt
     2941 agttctcata tactcgtcta ctttaattgc attagcatat cctagttcta caccagcatc
     3001 tctagctatt tcagatttag gtctcactcc agtttcaaga actatgaaat cactatcaat
     3061 ttcacgttta gtgttagtct ttataccctt aacatcttca tctctactaa ggatttcttc
     3121 tacacgttcg cctagtatga ttttcacacc attagcttcc aagtctcttt caacaatctt
     3181 ggccatcttg ttatcgagca tagtgggaag aatatgatta gccatctcta ctactgttac
     3241 atccatcata tctcctctcc ttcctgtttg gcctctcttc ttaaatgcct ctgccatctc
     3301 aaggccaata agacctgcac cagcaattat acatgaatga gcatgttttg ttattgtaga
     3361 atcattaaat tttactgcat caccataatt tcttataaaa aacacgccat tttgattaac
     3421 tcctttgata ttaggaacta ctgcccttgc acctgttgct gctatcaagg aatcataatc
     3481 aattattgtt tctttgccgc ttcgtaaatc tgttgtataa acttgttgtc taaatctatc
     3541 tattttatat gcacgtgtgt ttacaattat atctataccc tacttgcttt tgaaaacatc
     3601 tggaggcctt tctattagtt cttcaaaatt acttattata ccctctataa cataaggtat
     3661 gccacacgca gcataagaaa caatactctc ttcttgaatt atctttacgt ctgcttcggg
     3721 atcaattctt ctagcttttg aagctgcact ggttcctgct gcaacaccac caataaccac
     3781 tattcggttc ttcttgttgg tcgacaattc tgcagctttc aattaaggat ctctattata
     3841 tcaactgtgt tttctgtttt gcagtgcgat acaaaaaatc cgtagccgaa gataaagatg
     3901 ctctaaatgt tagcctgacc gaaatctaat tcatatccta gttggcttcc caaggcaatt
     3961 cttgactcat gtgtaactat ggtctggttg gaggattgaa tgatgaaaga tcaaattgat
     4021 tagcttgatt cactggtaat tcctttttag acagatcttc tttgatagaa tctaatatat
     4081 acagaccaag ttcagtcaat gttactttac tgtttaagaa atcatagaag taggaatctc
     4141 ctcttagtag aatactgtct tcccgcatca tttcagtagt atgtaattga gacgatgatg
     4201 aaggagaatt tgttttatca atggcaataa ctccctcaga taaagcatag ctaatttctc
     4261 ttcgctccaa atcatactca agtagttcaa gaattgaaag gcttgccaag tcgagaatac
     4321 tcactagtct gacaattact ggatttctaa taagttcagt aaggtgaact tgatcaattg
     4381 gtatttttct gtcactcata gcatattata tgcaaccgta gaagataaaa agaatgcgta
     4441 cgctgcaata cattgaaaat gctttttaat ggtttacaat agctactaca aattttgctg
     4501 ctatattttt tctcaataag agaactacaa taggacttgg ttattaaaac caaatgctaa
     4561 taacaacaag ttatgatcta cgcaaggcaa acaaacaagt actgagcaaa tccattgatg
     4621 aacagatatg gctaacgtta tcaacaatac agatttggat aaagtagccc aaactgttgc
     4681 aaatggcaag aaagataagc agagtctaag aaaaccagtc aagttgcaag ctgaatggaa
     4741 tttggatcca atcacgggat atcaatttag aacagaatta tcgtatgaga aaggaaagga
     4801 aataattgaa atagattcac cttcattttt aggtggaaat ggtaatagat taggtccaat
     4861 ggcgtattgt gtagctggta ttacttcatg tttcatagct acttttgcaa gtgttgcagc
     4921 aagtcaggga gtaaagctca cgaagcttat tgtcaataca gaatgcatga ttaactttgc
     4981 caagactttt gatatagcta acgaaccaat aactgaggga attaactttg aaataaaggc
     5041 tgaaagtgag aacgcagata agcagaaact tcaggaactg gttaaaatgg cagaagaaag
     5101 atgtcctgct atgtatagta tgtctcatgt gataaaggtt aatgctcaga taaattaaag
     5161 gcagtagtga tgcgacagta gagagtatgt gggacactgt gaggggcgac cttctacata
     5221 aaataaccta tgcttacaag tggcgtgaaa tggatactga atatcctatt ctattcaaag
     5281 atgggcgaac tgcagttgtt attggaagac tcgatatgta tgattggagg tcgaaaacga
     5341 tcattgatct taagacgacc aaattcattt aatggcagat aaaacaaggt tttctgccaa
     5401 aacttgaaca tatacttcaa gttcaatgct ataatactat tttctcgcaa ctactgccta
     5461 tcgaaaatct cagtatcatt tatgcagata tgagattgcg aatatcaact tagtaacaaa
     5521 tacaaataca aagagcaacc acagtagaat tggcaacacc actagtacca attcttctaa
     5581 ttcttctgat ttatcagaac cattatcatt atacaatgca ataccacagc gtcatacaaa
     5641 tcgtggaccg tatgacaaga ataaacctgt gacacaggat attccaaatg gaacctgtga
     5701 atttgaagtt tcttaagtat tcaaaaaaat cttcccccac atctttgaag acttcgaggg
     5761 attctagata agataattgt tcttcgttaa accttacatt cataagaaaa taaagtacct
     5821 gttcaagacc agcagctact aggtaagacc tatttattgg tagttttcgg acaaacattt
     5881 caaatatgcc ttttatttta ttttattatt attatcgtaa tctgtttgat tagaacta
//
LOCUS       DAOR01000262           10936 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_25990, whole genome shotgun sequence.
ACCESSION   DAOR01000262 DAOR01000000
VERSION     DAOR01000262.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_25990"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3467 a         2034 c         2055 g         3380 t
ORIGIN      
        1 caagggtagt tgttcgataa taggcgtctt cacgcttctc gactagccca gtgttacata
       61 agcgtttgag tttggtatag aactgcttct tgctaagctt gccaacataa ttggaagagg
      121 tgagtcttcc ggcatacgcc gttttcatta ggtttaccga cgtattatcg gataaaacgg
      181 tgaaaatctg ctccgttgtt acctcttgtg gcattccctg tctcagcaag agattttaag
      241 gtaacagcat atataaaagt tccctctctt ggtcattcat agagccgtta atcatacaaa
      301 cgccctgagt tatttaattt tatgagattg caacaacagt attttaaggc ggtagggcgc
      361 tgcgatggtg ttctttgctt tgaattcctt ggtcaaggca atattgtgtg tcctatgtga
      421 gggccaaatg aagagtctag tggcggaccg tcggccaccc ccctatgcta gctttagctt
      481 ttctttctga aaagtaactc cacaaagtcc tgtttcagat actaaattct ccgagaattt
      541 atcgctaaac caaataaatg agattttttt tatgagatct tgaaatcaat tattttttat
      601 ctacatccaa ttgtatcatt gagattatgg aagcaaatat gaatgacatg acagagcttg
      661 caggtccagg aatgctccct cctcgtcgaa ttgaaaattt aaccagaata ggaagatttg
      721 ttcttaattt tctttgtcgg catgagtatc taagccccat cacggtctac gatgagtcgg
      781 ataacaaatt tcgatagata ctttgcttaa gagtcagaag gagacgggaa agagacagtc
      841 agaataacta ggacgcaagt tttgctatga ggatatcctt tatcttctcg tcatctctta
      901 atagagtttc aataattttg tttcgctcca ctattgagag ttcgtgactt tgatctgggg
      961 ctatttcgaa cgaatcaatt gccttaagct tccagtagtt gttgttattg aatgcctttt
     1021 ctagtgtcat cgcagcatcg taaacgagtt cgccaaagac ggtaagagag taccttccat
     1081 tccttctttt aactaaccct gtctttagta gtcctgagat tctggaataa tattgcttac
     1141 gtgtgatatt tagtttagtt ctgaggatat cagtatctcc tttcgtatca gcgattgttt
     1201 taaaaaccgc caaagcattc tcattagaaa ttgcaccaag aatgttcgct aaagagatag
     1261 gttccatata attatgtgac ttgctgtttg tttatatgcc cgcttattat aaacattacg
     1321 aaatacggtt taaactttca aatcattaga tttttttgat cctagagctg aaattccggt
     1381 gtttttctag attatatcct atgtactagt ttgatcggat cagatagtac accggctccc
     1441 caaaaaattg gctcagacat ttggtgcgat acgtgcgata gcgaggtcaa cattattcat
     1501 taaacagtct gacatgttcg gccatcatac atctattgga aactacgtcc atgtcattct
     1561 ctttagcctt cgcctcagct tcttcattat aaattccttc ttgcatccag ataactttga
     1621 ttcctgactt cttgactgcc tcggtaacaa cggcaggtac atcttccggc tttctaaaaa
     1681 catcaactat atcgattaaa tcaggtattt ccgtgataga ttgatatgac tttcttccca
     1741 ttacctcagt agctgtagga tttaccggga taacattata gccacgttca ataagatatt
     1801 tggggacaaa attggagggc ttttcttggt ttttggacat tcctactact gcaatatttt
     1861 tgagagcgaa gattgttctc aactctgagt cggagtgaga atctgttttt attattggat
     1921 ttgccatact gccagatcca catctctatt tatcaatgaa gagattgccc tgactacact
     1981 aaaaaagact tcgttcacat aggtcatagg tcattcatat atacattaac atagatgtgg
     2041 atatattagc gaaaaggcta atctgccttc ttggccgtcc aagcatccaa tgaacgacgt
     2101 aacaatagtt gcataaaagt agcagtacca ttatattgtt cttgcagact ttcgtagaac
     2161 gtcaaaaacc ctgcatctca atagataaat agatattgca gatatggctc aaaaagagaa
     2221 aaaagaatta ggataaaagg cttgatggta attggtcaga aaagaaataa aatatgcaat
     2281 aattattgct cttggcatcg ctattatctt tcttggtatc cgctttgctt ttggtgtgac
     2341 aaatccattc tacgttgtcg ctagcggcag tatgataccc aaactcaaca taggtgattt
     2401 tgttatcgta agccacaacg tcccgtttga cagcttgaaa gtaggagata tcatagtgtt
     2461 caaaacattt ggaacgacag acacaggtca gcacgaaatt atagttcata gagtagctca
     2521 aatagtaact gacagccatg gccatagaat tatcaggact agaggagacg ccaacgctga
     2581 ttcgatccct ttccatgata tatgcttctc aactacaaac aagcaaaggc acagactgtt
     2641 aattccagct tcatgtctct ttaaagtgac aagaatacta gagaaccacc ttacaattat
     2701 attatataat aaataaatag tcagatctcc tgtttaatca gttgtttttt cttctttttg
     2761 tgccctagtt ctctgacttg ttcaataagt cctttcaact ctgatactga taattttgag
     2821 atactatcgt cacactcctg ccctcttgca tgtaacacct cttcggtcag ttgaacaatt
     2881 tcgtctttat gttcttcctc ttcatcagta agtatgccac aattcctgaa cctgaagaaa
     2941 ctgaattcac tcatttactc accaacaacg cacgcagaaa acaaactatg taattatgta
     3001 tcaaatatat attcaggtgt catgattctt ataacataac acaacgcctg agagatactg
     3061 gaggtaggct ctttctcctt ggtaaggttc atcgtttcgg attaactgca gtataatgtt
     3121 gatgcagtaa gaaaataaag gtattgcata cgcttagcca cttaccggca ggcgttcagg
     3181 gtaaggtcaa ggaggtgtct gaactccggt aggtactgtg ataacgaatt tacagttacc
     3241 tgctttagta tcaccgttct ttgcattaaa atctagaatt actccattac cattcgggcc
     3301 acagtcaaag tagttggctc ctccaccgcc tatcataaca tcatgacttg atccagcata
     3361 gaattgatca tttccctttc cgcccaccat aagggtatca ccaaaacctc ctatgaaaat
     3421 gttatttcct gaaccaccat atagctgcgc gctcgaagag ctaccagata taagtacatt
     3481 gtcaccagat ccaccgtatg ccttgcaatt gcctaatcca cattgtatga tatcattccc
     3541 gccaagaccg taaatggtag caccggtgac aggggtagca attatgatat cgtctttttg
     3601 agtaccgata atggctgtac aattttgtga acaagccttg ggtactatca gatttttagg
     3661 aatattaagg cctgaagttg cgtcttttgg catatactga tttttattgt gacctgagtc
     3721 tgagtgacga tacgagttac tgtatgagtt actttttgaa cttgaaatag acgtgctgtt
     3781 actataagac ggtccgatta agtgatgtgg gataggtagg aataaaccat taccgccgag
     3841 actaggcatt gcgtttgctg ccaatggtac tagtacactc gagagcagtc ctatcattac
     3901 tattatggga accattaatt tgatttgatt cgaaactaac aactaattat gaaattcttt
     3961 tgttcctttt tattggatat gacgcagagg ttaaataaat aatactagag ttcttagtat
     4021 aaccaaagta taggaaaatt gtctctttaa cagatgagtg aagtattgtc taaaagtact
     4081 acaactttct tgtgtcataa tcagatacta tgagaagctg aaacaaaaga ctattaggag
     4141 ctatggaagt aggtgcagat tattcacggg tacagcccca ctcaaggcga aacgcattag
     4201 caccaaggct tattccgccg acattatgac atgacacaga tcggcttatt cgtctgatta
     4261 accttgaaga cccccccttc atcaaacacg agcgaattga ataatgaaca atttacggcg
     4321 gtcgagcaag agtatgataa gagagattat cgtcagttat ctgtcaccct gcaaaagtga
     4381 catttgaaaa gtgaaaaaat aatgactgta ttcacttatg tcaaactaac tatttctgat
     4441 ttattgtaga ttctcatgct ccacaattat atcttatata ctataaattg catatctact
     4501 ttagataatt gataactcat tcgcgtaaag aaattactga ggtattgtat gataatgaga
     4561 atataattaa aaaaacgcta gaaacttttt catggataca gagaagcctt gaaggctgtg
     4621 tcgatcatac agaggtaagg atgcatgtta ctacccaacc aatctgggag ggtttgaatc
     4681 aactcaaaga aagaggagtg aaattaagaa tggttacaga gattacaaat gataatattt
     4741 cttatacaaa aaaaatgatg caaattgccg aggtacgtca tttaaaggga gtaagaagca
     4801 gctttggaat agctgatgga atccaatacc ttgaccatgc aatttcagat aacaaccagt
     4861 tgtcacatgc aatcataagc tatgttaaag caatcgtcga agcaaaagaa tacctatttg
     4921 aaactctctg gaatttagct attcctgcgg agcaagcaac tatggaaatt gaagaaggga
     4981 tcgaaccatc caagactgag atcattcaag atactaaatt atcaatagct cgctctatag
     5041 atatcataaa atcagcaaaa gaagaagtat tagtgaggcc acttctaaga cgtttgctat
     5101 aggtctgaac gaggccctag ccgagctttt tacagatgca actcgtaatg gtgcaaaagt
     5161 aaggctactg attccatatg gagagagtat agatgataca acaaacaatc taaaaacggc
     5221 tgtcccgcaa gtggacataa ggatcgcaga taaaagttta gagacaaaaa tcacaattct
     5281 gatagtggat aggggagaag taatgacgtg ggaactgagg gatgacaatc tcgaagatcc
     5341 atatcaagca ggtgcgaagc aacatactca aataataaat ccattgccac cccgtatgct
     5401 attatttttg aaaccttttg gaaacaaaca gaattgtatg cacaatcaca aacttacatt
     5461 aaaatgcaaa acgagtttat caatattgca gctcatgaac tacgcactcc ggaccaacca
     5521 atcattggtc tttcacaaat ccttctttct aaaagaggaa agatagaaga tcatattgaa
     5581 ttgatacaga caattagtag aaacgcaaaa aggttacagc gtcttacaga agatacgtta
     5641 ctaagattga aagccactcg ctgcacctaa ataacgagac ctttgaacta agtgatctaa
     5701 tatcaaatac tgtcagagat tttggcattc aaattaatgg cgaacataat aaaagactga
     5761 aaataactta tgaggatgaa gcaggaagag aagaccaaaa gcaaagtagt tttattgtaa
     5821 aggcagataa agcgcggata tcgcaagtta tcgttcatct tttagacaac gctattaagt
     5881 ttaccggaga aggttccatc accatcaatt taaaaaaagg aagaaagtag tagtaccaac
     5941 actaaacata atcaaacaat tggcgttgtc agcgtaaagg atagtggttc gggaatagat
     6001 tccgatataa tgtcaaggtt gtttgcgaaa tttacgtcga aatcatttca aggcacgggt
     6061 ctgggtttgt tcatatctaa gaagatcatt gaagctcatg gtggtaaaat ttgggctgag
     6121 aacaacacaa atcaaaaagg agcaacattt tattttagct taccaaaatt tagtaacatc
     6181 agaaaataat agtactaaca atgagatgtt ggagttctat aacggctaag ataataattt
     6241 agcgactatg ccaacgacct tttggcaagg atagctccat ctataccgag cgtcgtaaaa
     6301 aggactttgg ccgatggtga tcattacgta tggctgccat aattctaaat ctagatacca
     6361 taatcgttct acaaaagcta cattgataat atctctgttc tctgcatcat tttatgatta
     6421 gtttttcatc tgagattgta gtacattaat ataactactg taaaaatggt ttcaatttac
     6481 acattctcct atgtcttgag atttatttta tattgggctc tgtgaggctt gttgtgagat
     6541 catgataaaa cactctgtag aatgtgacga atataattat atgtcacacc gtattttgct
     6601 tgtctaatga aacccggggt agatattcaa ttattgcaga gtgtaagaca caaactgata
     6661 gccaaaaata tcactttaca gagtataaaa caacgacaga ctacaactgc gcttataaat
     6721 caattagaca tacctgaagg attaaaagaa ctgataatca gtcatggttt tacattagat
     6781 ttacttctag gtttacagcc aactgatttg gcgcaaacct ttggaattga taatgatgtg
     6841 gctaagatga taattaatgc agcaagaaga aatgtgaaat atggttaaca gtgcatatga
     6901 agaggacaag gataaagttt cagctgaaac gatcagcgta gcccaaataa atgagaagag
     6961 aatgaaggtt tgtttcttgt gaatattgtc attgctggaa aagaaaacta ctttagtttc
     7021 ttttcttctg attttctttt cttataatac aatactaatg attgtggatt ggagatatat
     7081 tcttccgtga ttatctgtaa agtgcgtaac acaactgctt ctatacttgg tttttccagg
     7141 caatcttcct cataaagcct ttttgctatg gataaagttg agtcatattg tgtttgtgat
     7201 aaatcaagct ttaatatggg tattataaat taccttgaga taacattatg tttgttatta
     7261 aataaacttt gatgtagttg gttgcatgaa tgtggttaaa gcatatcttg ttcaggacga
     7321 tcacttgggc ttgagcttgg gcaatgcttt tgtctgatta atagatgcaa catggttttc
     7381 gtggctggta agcgatgaag acagcttgca attaaccttt gactgattgc acacaaacca
     7441 atgttcacat ttatcgcaat agtgaatatg atcaaaatcg taatccagag atgactttgg
     7501 atttaatatt ttaaactctt aacgcctcct taatactcac tgattagtca gttatttgga
     7561 tttatctacg ttcaatgcta gttaaatact tggaagccat ttaggaaggc agtcctctcg
     7621 cgagctttta cgtatgcctt ttcctgcatg tcgcgtactt cttccctgcc tggggcctat
     7681 gctcgagaca gcaccttggc ttattccagt cctgtatgct atctcatttc tggacactac
     7741 ttggggagaa gtatgaagat atttctcata gtctgctgaa tttcaggttt catttcgctt
     7801 acaactggtt gaccacataa attgttattg gtcatattgg gcaaaacaac cgtcaaattg
     7861 ggcgtcacat tggtcaaacc caacacttgg agcatcttat agtaagccct acgcgatcat
     7921 cttgttgcgc ctagttaaga agattccaca tagcttattc aaccaagtag aaagatatat
     7981 gcctcaagga gcgtttcaat aaggtaaagg gagagaggac agcccctgtt tgagcagatt
     8041 gcagatggga atagttcgat taatgcgatt atatcttcga tttatacctg ggactacgct
     8101 acgatttaca tggccgtatc acgatatttg gtccctttcc tacacctgct gtagatactc
     8161 aagagaattt agttaatttg tgattgcgta aaaatgcacc gagtatcctg aattcaaatc
     8221 taggatagta tgactgcagc tgtagttatt tgacgatgga acttcatgcg gttattttaa
     8281 ttttaatatg gtaaagggac ttctcatgaa acacatcaga tgtgctaacg tatgccgaat
     8341 atgtgcttcg gaatgtcgta aaatcgcgcc atttagatct cttccctaaa ttacgaaggc
     8401 aaccaaacga ccataagatt gaagggtcaa aatgcctact cgaaaagcag aaagagtcaa
     8461 atatcatagg aatattgctc tgcattgtat caatggctag cgtttttaat gatcattcgt
     8521 ttcccctcgt tattattact attattatca tagtagttgt aggcttaatt gtatctctaa
     8581 gctccattcc actttatggt gtgaaaaatt acttgttccc ttctttcgta cttacaacaa
     8641 atgaggctaa ctcgctcagt cctacttcag cgtttgcttt atctgaaagc cctaaactaa
     8701 gccagctaat atttcacatt caagaattca ctatacctgg caacaatgac agtatacctc
     8761 tatatcctct gtatgataag aaccgaaacg caatatgggt aggagatact gcaatagata
     8821 gtagtaggat tttagcattt aatttaaagt caggcaaatt catggaacat aaacttaatg
     8881 gtactagtat tgtgactgtc atggctttcg attataacaa tactcatatt tggtatgtag
     8941 atccattgct gaaacgtctt ggacattatg acccatctgc taacactacc aaattataca
     9001 acattccaac caatggtact atttcacgta tcgccatcga tctaaaaaat aacgtttggc
     9061 tgacttcgcc gaatactaat gaattactga gatttaatac acgagaaaag aatttcaccg
     9121 tattgaacct accgacaaca aatgcaatgc cgataggtat tgcagtagat aaatcatcag
     9181 gaatgatctg ggtagcagaa gggataggga aattagcgag tatagatcca ataaagaact
     9241 acaaaattaa tgaataccct gtgttagccg ctaataatag caaccataat aacaatacgc
     9301 actatactcc tactgagtta tttatcaggc cgtctgcaag cgatgatatc tatatttctg
     9361 ggcacgataa tcatacagtt tctattttta atataatttc taaaaccttt aagacctacc
     9421 ctgtatttaa ccccaaagca ttgccattcg gaatggcaat ggatatgagt ggttatctat
     9481 gggtagctga acatactaca aataagattg cagtaataga ccccaaaact ggctcaagca
     9541 gggaagtagt aataccgaag ccaaatccct atgttcagtt tttgacctca gattcgcagg
     9601 gaaatatatg gttcgcagaa cagctaggac attctctggg tgttataacg tcttcaccca
     9661 tcaagactcc gtagatgatt tagtttgcga ggttcatacg cgaacaatca aacgatccca
     9721 ataagtttag ttgattcaag aaacactttt gactttttag tttttttcat tttcttgtcc
     9781 ttctttgtat tgctattttc ccctgatact tcaaaagact atattgttat tactcttctg
     9841 agctgcgtct ttttttatct cttttcacta taattgaaaa agttgctatc actataacac
     9901 caattattcc tgctaatgct gcaggaagaa gcaatggaat cgtatagttc agattgatgg
     9961 taccaagtgg ttgaaaggga acaaagaccc tgaacgatgc caatgcgatt gcataatcct
    10021 ttgaattgat cctaagaatc acttgatatg taccctcatt cgggaaagtg gaatttacag
    10081 aaaaatcacc atctgaaacc gtcaggttat taaatttgaa aacacgctgt ccatttatta
    10141 ttgtaacctt tgcaaacaga tctttcaaat gttgaccagt ttgtaaattc tgtacgctga
    10201 attttagttc agtaggactg ccaattagag gtttttctgg ttcgtaggta aactgtattt
    10261 tcacgttatc ttgtgggttt atccattgct gggttctatc accagttata tgtgcataag
    10321 caaaatttgg gagtatgggt gtagctatcg tagcaattaa gaaagcacaa gtaacagtaa
    10381 ataataacag agaaccaatg cttatcgaca taacacctca ccgctactta acactattac
    10441 agcaagagag attatatttt agttttagcc atcgtcaact agtaataatc taataatgac
    10501 catgcagcgc tcatatcgag agaggatcta catcgtcaaa gatataatcc ttaaactggt
    10561 tgaatatggt gaattgaatc agactgcttt aataagttgc tgtggattga attttaaaaa
    10621 acattcacct attctcggca gattagagac aaagggattg ataagcaaag atcaaataga
    10681 agtcggaaaa agaacagtta cagcatatag accaactcaa aaaggaatag aattttctaa
    10741 gcatatttta gagccttatg agataatgtt tcctcgttca gacaccatac cggcaaacaa
    10801 tttggcattt ctgctaatct tgatctaagt catactcgga tgcaatattt ctgtctagtt
    10861 cttccttttt cttggaatag acgctatact ttttattcca agaattaatt gagcggtact
    10921 gtttaattcc caccac
//
LOCUS       DAOR01000263           15124 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_41988, whole genome shotgun sequence.
ACCESSION   DAOR01000263 DAOR01000000
VERSION     DAOR01000263.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_41988"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1506..1591
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4948..5082
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4418 a         3042 c         3160 g         4283 t
ORIGIN      
        1 acaacaatga tagtgtttcg caacatttat ggtgtgatga tacgacgggg gtgtgttgca
       61 gagcatagca gattgccagt ttgtttgtag gagggatatt caggaaggtg gtagaacatg
      121 gtgcaagtta tggataaaag atggcggtga agctaatgag attgatctaa acgggaaagt
      181 tagcacatcc gaagctactt cattcaatat gatcgtagga gaaaatagtc cgctgaaaga
      241 acactttgag atgccaattg atcagtgtgc ctatgacacc tttgtcggag ctagagctat
      301 tcttatacag ggcctaccgg ttggccagca tagagtacac aggaggattc ggaagaggcg
      361 cttataaaag cgactcggtc catgacttca tagttaatcc tttgtcgagg actggaaaag
      421 atttgatacc taaagcatcc tagaggatga tttgatacct actcataagg atttggctgc
      481 actctaagtt ctctcgaagc cggcccaatg ctttagatgg gctcactttt cggtaatgat
      541 agcgttttgt tcaggaaata agctatgcct aggacattgc gttgcgtgct gggaagaaca
      601 gttcgtggcc gaaaaagtct tcgttattaa tgtcaaataa taataataat atgagatggt
      661 tgattgcagt tgtactccta tctaacatcc atcttctcaa agcgttcatc atcgcagatg
      721 agatttcgcc aagtagtcct gctgcttatt ctttagttct tgtgagtaat cattttgtac
      781 tttttaaacg tagtacgtta aagaaacttc attgctatcg ccaatatcgt catgttaagt
      841 caccaagcta cgaatggacc attccctact gtccagatga attttttttg tgtatattac
      901 aggaacgagt gcacaagaaa tcggaattaa gagtcatttt agtaggagaa cgagttttcg
      961 ctgctgaaat agattctcat tctaatatca atgcacagga tgatttgcat aggagtccaa
     1021 tagttgacct tccgaagaaa cctattcaac tggacgatgt gactaccgat ggttgcctca
     1081 agattatgaa aactcttgac cttaagtacg gagctatcga tcttattttg gatctgaaag
     1141 accaggtaca ttttctggag attaatccaa ccggtgaatg gtgttggatt gagcatcaaa
     1201 ctaatctgcc cataactgac gctatggtag aattaatagg aaaccttgca tcactcacgt
     1261 aatcttctca tgtataaagc cgctctcttt tgtagttcgt gaagttttta attctaacgg
     1321 tagccctcaa tttcgatgcg gccattgcta acgatgctca aacattgagc agatttgcag
     1381 ttgttcttca ggtttccggt tcggtattat caattttaca attgcgaatt gtcagttaaa
     1441 gtagtttcgg ttgcaatttg catcgtgcca cttttggtgt ttttgtggaa tctaatgaat
     1501 ctaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctaatgaa tcgaaaccac ataatttccg
     1621 tgtctacaaa tattgccgca aatggagaac ctgtttcaaa tcattttcat aaacctgtga
     1681 caatagaaac caaattataa tattacaatt gtctgggttg attttcgaga aaatacctat
     1741 taagttcaag taagaaaaca aaggaagcca tgttaggtca cgcaacgatc atattcttag
     1801 ttcccatttc aatctcgtta ttggtagttg cgtatccgat tttaacatat gcttcgcctg
     1861 ttcctcattc cctctccatt cctctgttag caaacaacca ggatatcaga accaacgaga
     1921 ataatccatc aaccttacag tgagtggaaa tgtacaaaac acaagattgg aatttcatat
     1981 attaacagac ccacgacatg gtttattaga aggtgaacca aaagcaacca gttcgaactc
     2041 tgcaaagata gtgtacaagc ccaacataga ctacaacgga acggataatt ttacattcag
     2101 ggtgaacaac ggaaatcaaa caagcaacct tggtactgtt tctataaaca taaaatttgt
     2161 tcctcacagt gtgtcatttt ggagtgctgt agtcactttc atttcgtctg cattttttgt
     2221 tcttgttata gttcttgttg ctaagtggat gattagaaag tttcgcaagc atatcaaagc
     2281 cgacaacaat gcaaatgatg gtccgagtat tccaaaaagc aggtccctac cagatatcat
     2341 tgctggattc accaacatca ttagagatga gagtctttat ccgagccttg ccatctttca
     2401 gttttttgtc tggacgctgg tgattagttt tgccttcttt ggaatatatc tgattagatt
     2461 tattggtggg gttccaatat tatctggtac tgttatacct aataacctcc taattcttat
     2521 gggcattagc gccgcagtcc cagttgctaa tgccgcagca tcacaaatca agtatggagc
     2581 taagaaggct ccgaaagtct tgcctccttt ttcaagtatg cttatggaag gtggtaagcc
     2641 taccttggct agatttcaaa catttgcctg gacatggatt accattgcta tatacatgat
     2701 atttttgttt tctcagacat ttagccgatt ggatatagta gagcaactta gcgtgcctga
     2761 cattaatcct gtatttctgg cattgatggc gataagccag ggagcatatg taacaaacaa
     2821 aattagtacg ggagatgttt tgtccattac agaggtcata cctgccaccg gtaagaaagg
     2881 ggaacctata accattctcg gcactaattt cggaacggat caaaaagatg ctgctgtttt
     2941 ttttgaacca ataaatcatg attctaaaaa tatcacagta gacaaaattg agagttggag
     3001 tgataagaaa ataattataa ctgttcccga aaaaatcgaa ccaaaaacat ataatatcag
     3061 agtcgaaaag ggaggatcct ctcccaccga tacatctacg actacagagt tcaccgtaga
     3121 gaaggaatag attgcaaaag agcatataaa gaagaaattg gcacgagtgg aagtgacact
     3181 tatattgaat atccagctta agaaaatcta tgttatttat caccatccat gaattacaca
     3241 gctggcacgt tagtttcgat acttatcttg caagtaccac agcaggggtg ataccattct
     3301 taatcagtac acgcctctat ttgatgagaa tgaagttgaa ttaataacat cggtgccgac
     3361 aaagtgcatt ctagaactaa tgacataaga tacccaaatt aatcacaggt ctttaaattc
     3421 tttttttata gaataacgga accaccgtat caatgtgaaa ttctttactt gcaaagcctc
     3481 attctcgctt gtaattcttt tttttaattt tttgcgcttg atttctattc ttgccttccc
     3541 cttactctaa ctaactcttc caatacttta tctaaatacg attttatttc attgtctgtt
     3601 agtgatggct ctactccact ttgtgcggtt aacactgatg cgttgtcttg tgtcatcttc
     3661 atcactttat cctcaatttc ctttgtcatt tgagcggtcc cgatactgtc tagtaatttt
     3721 gatgattgtt gtagggtaga ggctttaatt gactgacgta attttacatc ttgtgctaca
     3781 gagattgctg aattataaag tccggttagt atcataaagg atgctaaccc tacaaatgag
     3841 acattagcca gtccataggg tggataaccc gcttgcagga cactagcctg agctgcatta
     3901 acaaagagga caaacccgta agcagtaata accatataat ctttcacatg actgtgctga
     3961 cgaagagccc tagaaacaga gcgaaatcct cacgcagcag acaaccttaa tgaattacaa
     4021 gttcatatag aggagatagg gaaccgtctg ccacatgaac gtgagtatct gactcatgag
     4081 gaactggagc gctcccatgg tgcgtcacca ggtgacttgg accaaataaa ggattttgcg
     4141 cgcggccatg acttggaagt aatgagtgct aatgcaataa aacgttctgt ggtgcttcgc
     4201 ggcaccgttg gttcgatttc ctctgcattt ggagttaaat tgaaagatta caattacaat
     4261 gggaagattt accgcggtcg aaagggatgg ctacacgtac cggacgagat ctcttctatc
     4321 gtccggggcg tacatggcct agataatcgc caccattttg agcgttataa tcgacgcctc
     4381 gtaaaaacag gcagaaatcg caagtacact cgggctgcga gaggagagag tcacttcgtt
     4441 acactcaaga aggtagcaaa gcgttataac tttcctacta gatctcggga tggaaggata
     4501 ttagacggag ctggggtttg tataggaata atcgagacag gtggcggttt tgttgctgaa
     4561 gagcttgata ggtattttaa agaccttaaa attaggagtc caaacgtatt ggctgtatcc
     4621 attggtaata gtcagaataa tcctggtata gacttagaag cggatggcga ggtcatgtta
     4681 gatataggaa tagcaggtgc aattgcgccg gcagtaaaaa tcgtagtcta tttctgtgat
     4741 ggcacaacta atggttcaat tgatgctgtt aaacaagcga tttatgacag ggaacacaat
     4801 ccttcagtga tctcaattag ttggggtgct cctgaagaca aatcccgtgg aagtcaatac
     4861 atggattcga tgaacgaggc attccaagat gcggctgcgt tgggaaaaac aatttgttgt
     4921 tcttccgggg ataatggatc gtctgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccggggat aatggatcgt
     5101 ctgacttaag ggcgcaagat ggagtgctaa gtgataatct agcacatgtt cattttccag
     5161 cttctagtcc ttatgtcctg gcctgtggag gtacggaact ggatccatcg gccaatgaag
     5221 ccgtgtggaa tgataaagat ggagcaaccg gcggaggagt gagcgaccgt tttccaagac
     5281 caggctatca acagggacta aaaatcccgc cctcagctaa taaaacacgg tttaatggtc
     5341 gaggtttacc tgatgtagct tgtctagcat ctcccggcta caatatccct gttcatggga
     5401 aggtagaacc aaggggtggg actagtgcgg caacgcccct ctgggctgga ttactagcac
     5461 ttttcaacga gagtattcgt aagaatgtgg gatttcttaa tccattactt tacaggcgat
     5521 ttggtccagc tggtgtcctg aatgacgtca aaaagggcaa taataggaca aaaaaaatag
     5581 tggctaaacg ctctcctggc atgaaaggtt ttactaggat gaaagggtac aaagccgcga
     5641 gtggttggga cccctgtaca ggttttggca cgcctaacgg catgaagctc ctgagagcgc
     5701 tcgaaggaat atgaatttcc tatgttctcc tcctaaaact caactactct gtggttatag
     5761 ccaaactgaa accaaaagtc gctcctttat catcattaat ttcaccccat atcttacctc
     5821 catgagcttc aatgatccgc ttacaaatat acaatcccaa tccagagcgt tttttaaatt
     5881 ggaaattaat ttgatcgcag aaaatttcta gaagttcaag agttagtgaa taaatctacg
     5941 actgagttag ataaaggaga aaacaaccat ccgcatcagg gagaattcta acaatcttag
     6001 tcattcaatc agtacgccaa tacagacttg gtatcggtct acctcccggg aggcactctt
     6061 taagtgccgc tctatgtact tgaattaaaa acatatacat taaaaggagc ctttagcacc
     6121 aaatagagca ggcggttttg tggaatccct ttaacgagga tagtggcact ccaggattca
     6181 aaatatggag aattgttcgt atcgccgtat ttgtaataat tggcctaatt atattttggg
     6241 tagccagtag ccagtctgta aaccttttct tgaacgttgt tgagtttggt aacgttttca
     6301 caaaaccatt gtattactcg attgtttccg gtctgatatt atgcagcatc gctgcagtac
     6361 gagtaaatta tcggcacaga aattccatca cttggtacgg catccacctg cttgtcaact
     6421 ttttgaagcg tggcgataat gattcttcat cttcgtcctc agcttcatct tcgtcttatt
     6481 attcaaaaac tgcttccaaa cgttccaagc tgccaatacg gtatccggat ttcaaaatgg
     6541 agaagagaag ttttataata tggcagataa caaaggtggt tttatttgca ccgttattta
     6601 ctaacctgat atttggaatc agtgccgtgt tcttactgga aggacacgat ctaggacttg
     6661 gactacttcc aaacattttc ctagtgcctt ttacaaacat tccatcagat ggcagtttcg
     6721 cccagcagca tgtaataccg cttattcccg tcctgacgct aataattccc tctcttttat
     6781 cagcaattgg gattcgaatc tttctttaca tcggcgttgc aggagggatt agcatcgcat
     6841 caagatacct agcagatgcg tcagaaagta aacccaaatt attgtcatat atttcaacaa
     6901 tggaacaaat ccttggcgtg acactcttgt ggaccggctt cactatgttt ttcagcttca
     6961 atattaatta taatacaaag tacgcgatat tgggaactct tatcatgggc gcctttttca
     7021 ttgcgtatgg tttcctggat aacaggcgct caaagataat agtaatacca gaaagaaagc
     7081 agatttacac cagactgttt actattctgg caattacaat tgtcacaggc tcacttatgg
     7141 ctatcaataa cactatcgcc gatgccaaga aaattcaatg ggggggaccc tacattgccc
     7201 aggaaattgc tgtgaatcgc tacatcgccg aacttgacaa agtgcagaca gtaaactaca
     7261 gtctggctgc ccctccacca ccatcttccg cctcctcttc attacctccg cccagcattt
     7321 ctgcaatggt caacggaaat aatgatgctc taaataatat cagactatgg gatcagcaag
     7381 cggcgcaact caaactcaag ccgcagttag gacagaaaaa tgacgttaac tttgcagata
     7441 ccaatatcgt gcgcttcaat aacactatgt attgggctgc cccaactgct ccgaatctgc
     7501 ctgcagatgt cactcctgaa aatatttggt acaatgaaca ctttgtatac acacactccg
     7561 gaaagagcgt gctgatgatg gaagccaaca caggcaatgt aatagattcg agcaaattct
     7621 tcaaacaaaa aaatatctac tatggcgata gcggtcctac tggaatcttc aaccaagcct
     7681 ggtctgcctt cccagtaggt agaacactca gtaacgagat cgaaggcgca ttttatactg
     7741 gcacgggcgg cgttaatgta gctcctccgc taagctgggt atttgagccc aaattcatgc
     7801 tttcctaccc tgatagtacg atgcatgtta tgcgttacaa agacatattt gaccgaatga
     7861 aagtcatgta tccttacttt gtttatgact ttacccaagg aacttctcaa gccaatccaa
     7921 acataagaaa tgtaggagtt gtacctgtta ccgatggaag gaacacgtat tggttgatgc
     7981 ctcttatcgt ttttctagat actagtcacg ttccttggag ctccggtgat atgttgaggt
     8041 tggttggatt tgcattaata gatgcgtata acggctctgt gaaaataata aagaacgttg
     8101 ctagtggtaa caacgatccg ttctcgcaaa ttttctttga acaatatgcg ggcaataatg
     8161 gtggcgacgg aaccgtattg actaaaaccc cagaatggct aagcaagcaa cttgtatatc
     8221 cagaggagat gtttttgtgg aaaattaaca ggtttaatca atatcatgtc actgatccca
     8281 agacattcat tgaggcccaa cagttctata aagatcctga ccaggtttca acaaattaca
     8341 taattactaa acctcctggc tttgataaac cacaattcgt gggaatgcaa gccctgcaac
     8401 taagtaattc tcctgctaat aacctcgtag gatacatggt ggttcaaaat aacctggaaa
     8461 attttggtaa gatgattttt tattcagttc ctcttgactc ggctacaaag atcctaggcc
     8521 ccggtgcagc aagagatgct ttggagaagg atcctgcata tgcaaaagag aagagtttgt
     8581 tacgcaacac acgagttggt gacagcttac tttacagaat aggaaaccaa gaggtttact
     8641 ttattcctgt gtacacggct agtacctctg gtggaggtgt aattcagctg gggaacattg
     8701 ctgcggtagg agcttcagta accgggaacg cttacgttgg tttgggcgat acgccccagc
     8761 tggcttttga aaattatcta ctaaaggctg cgggacttgg tcctttaaat cagacatctg
     8821 tcggtacgac cactatcgca aacggaacat tgcctgcagc cccctctaac gccagcgcta
     8881 caaccaaaca acagcaacaa gcaaatgaaa ctctagtcct ccacagggca gaaagtatac
     8941 tagggttaga gaaaatattt gtctctgcag gtctaactgt tctaaagcca acagctgttt
     9001 ccgcgccgct cagctttaag gaggctgatg tcacctatat agcagcttcg cagatcgatt
     9061 ttgtaaaatc agccatcacg aaatttctgc aagaattttc gtcgcccggt ccaggaggtt
     9121 cttcttccgc tagccctggt gccgctgcta tacctacggc tctgcgaatt tttgaatggc
     9181 agggcagtga caataaagtt gtgaactttg ggatactaaa ggttgttaac ggaatagtgg
     9241 aaaatcatta tatttcgatt catgtaggat agcttagttg attgatagct cgattgaata
     9301 gttgcttggt taataggcat gaccgataag gaaactagga tgcaaacaac tcttgttgta
     9361 gataacctat ctcccttcac accagatatt gtgagatgtc ttaacaaact gggagcaaat
     9421 tatatccaaa agagtttttc cagccttacc aatgttacta tcagcagtaa cgacgataac
     9481 aacagctcca attacaaata ctatggtcat aatcccggcg acaacgccta tatcaacagt
     9541 cagaataagg gtcttggcga tggtgctaat tacgataaga agaacaataa tgacagtcgc
     9601 gatataatcg acacgaataa caatactaat aatactttat cttgtgacaa agtaatttta
     9661 tctggcagac gcatgaacaa tcgagagatt aatatcgtaa attccagcat cataaggcaa
     9721 tgttttgaaa ataatatacc acttttgggc atctgttatg gtgcagagat tttggcgtta
     9781 acgcttggcg ggactatccg caagatggct atgcctgttc aggacactat ttctttaaag
     9841 gtgtcgaaat caggctcgct aatttctgcc gatatgaaga cgcttctcgt ttatgagagt
     9901 cataggtatt gtgtggcaaa gttgcctgaa gactttgagt ctcttgcatc ttcgatacat
     9961 tgtgagcacg agattttttc tcacaaggag aagaaattat tcggtacgca gttccaccca
    10021 gaaaaaagtg gagcagacgg tcttaatctg ttgtcgaaat ttctaagtgt gtaggccaaa
    10081 ggattatcgt taactatcat tatctttatc ttgatctttc gtgcggttcc tattttctat
    10141 actattagtt ttctctctcc ttgtctcaga cttattccga caacaagaat gatttttatt
    10201 tgattaaaag cgctatcaaa tccagagtgc aatctcaacc tgaagatcat tcaataacac
    10261 ttccacttgt tagtaaggag gaagaaaaca tctgtcttcc tctggttatt aatgtggttt
    10321 cgaaatattg gggtgaagaa atccaattag aggaggctat ggccgttgcc aggaaatatc
    10381 cggggatgaa gggttcgata atgatggagg gaatagagct tgctgaaaaa catggcttta
    10441 cgacctacat ttatagagga tcaataaagg atctgaaaag gcggatagac cagggtattc
    10501 ctccaataat tatccttcct ggaattcacg aaacagtgca acatgccacc atagtatctg
    10561 gttatgattc tgaagaacgc agaatcctaa cttatgtgcc agaacctgat acgatagggg
    10621 caattccgga acctaaattc gagcaggatt gggaacaaga cgatatgatt acattggtga
    10681 tagtcccaaa cgatatgaaa gatattttta aaaatgaaga cctaaagttc cttgattcaa
    10741 acagaatctg ttttgaagca gaaaaacttc gtcagcaagg aaggatcaat gatgctatag
    10801 aaaagttgcg aagagcagcc aagttggacg atgacaaccc acaaccatgg tgcttgttgg
    10861 gagggatatt taatgagatt aattcagaag acgccgtttc ttgctacgaa aagacgatca
    10921 cactcaatcc cagatattat cttgcttttc gtggcctcgg aaactattat ttaaagagca
    10981 aggactatct taaagccgaa gattattaca cgaaggcaat cgctatcaat ccgtatagat
    11041 ttggaccaat ttacaaaaat cgcgccttcg cgagattaca agttgaaaat aactccggtg
    11101 ccaaagatga cctgttaaag tatctcgaac aaacacccaa cacagcagat aaaaagagta
    11161 tcgaagatgc tgttagtcag ctctaatttc cgtacttcac cctggtgttg aagagttgaa
    11221 gttgaacatc aggaagaagg gtcgcgggcg actccaagat aagtccaaga tgaataaaca
    11281 aggcaaaact gtattgatag tatttccgtc tcagttctct ataggccgaa ttgataaact
    11341 gattgccaat attaaagatg cccttcgatc aaaagatgtc gggcttaggg atatcgccct
    11401 tgaaaaagaa tgtctcgttt ttgagttgga cgacgtagta gagggcgcgg cgattacagt
    11461 agagatgttc gggattgaca aggttgctat cgcaaaaaag gtgtctactg cacgttttga
    11521 agaaatctta gcagaagtcg tgagtattgg aaaactgaaa attttgccca aggagaaatt
    11581 ctttgttaaa gttcagataa gcaataacgc aaaagtcgat tacaagccaa gagatctcga
    11641 gtttgcttct acaggcgagc ttacagcagc attgcaatcc tcattaccgc catcgttatc
    11701 attatcctct cggaaatctt ctactttttc ttcttctgct tcttcacttt cctcctctgc
    11761 gcgcccggca aaaaacgagt tagacgccga tagacatatc gagatattca taggaaagaa
    11821 atcggcatac gtttgtattg atattgacgc aggtctaggc ggtctacctt ttgcatgtca
    11881 agaggaaaaa gtattgtgta ccatacacaa cgccttatca gctatttctt gtgtggcagt
    11941 tctaaagtgc ggcttctttc cagagataat aattctttat actgacgacg acgacctgag
    12001 acaaaatttg aagatatttg gcctggtagt taatagaatg aacaccaaga attattcagt
    12061 tcgactagcg aaagtagagg aaatacaaaa accacagcag caacaacaac aacgacgcga
    12121 aagcagtcat aaaacaattt tgctagaaat cgttgcaact caaattctga ctcgcatgag
    12181 agggaaagga gtcgtcgtgc ccttgagcac cgccatccat cctctttggt tgatagaatt
    12241 aactttcaag aaaatcttgt cctcaaacaa gatcccctgg atgcctttgt tactgccaac
    12301 ccaggatata tatgatattg ccagggaact ggggatggaa gaacataaac tccggctaat
    12361 gtcagaccag agttgtatga tagatgggct gttgacgttc acgaaacaag actatgcgaa
    12421 atgcaggaag tcaatagaca taatgacaga atctgctctc aagaatatga atactatctc
    12481 atttgagata ggaccgaatt atcttcacga cattctcgat tccgtttagt ttttctgtga
    12541 gtttcattag tacgtttgct ttttgttggt attgggaatc gttgattacc tcatacatcg
    12601 catccgtaac ttgacttgcc ttgagttctt ttgcgtccaa cataatacct gcgccaattt
    12661 ttgctatttt cttggagttg gctaattgtt caccatgatt ctctatggga atggtgagga
    12721 tgggtttccc aaactgaatt gcctgcgaca gagctgcgtg tccgcctcgc agtaccaacg
    12781 cattactcat tgcaaagatc tcgtctcgaa ctgggcacca ttcatagtac caccctgaac
    12841 cacatagttt cttggggctg gtgtctcctc tcggattccc ctctgaaatc acgtactgaa
    12901 tttcagatcg aagggctttg caggcatcta ttgcggttct gatcagcggc attcgagtat
    12961 tgctaggacc gctgatgtga ataaaaataa ttggtctgtt tctttccaaa ccaagaaatt
    13021 gagcaacctt gctgatttgt tcttcgttga cgtgaatttt tggtgtggta aggccgacat
    13081 attctaattt tccagataca gtactgatgt cccaaatgtt gtgctcggct agcgtatacg
    13141 gtggaggcag gtcaggaaca agaattttgt ctgcaagcga ccacatagta cccagaactt
    13201 caccgtttaa tctctcgtaa atccttgcga tcttgagctc ccttaatctc ggtgagagta
    13261 ataattttaa ctgattcaaa ataactatag aaggaattcc caataattca gcagataaaa
    13321 gtgaagacag cctcgtatct gagacaatga tgtcaggctc gtattctaac atattgcgaa
    13381 cttcggtatt cacttgcctg cagaaatttg tgaataaata aggaagtttc gctatgctgt
    13441 cttgtataga aaaacctccg tcaatgctcc acgccaattc aactggcggg attggtagac
    13501 aatcgtatcc atgcatcgag atatagccca cggcctctcc agacgaggaa aatctcaaat
    13561 tcacgcctaa attctgaagc tgattggcta tcattattaa tctgcttgcg tggcccagac
    13621 cgactccgta tggtgtgaga tatgcttttg gctgcaagtc agcgtcgata tgagattgag
    13681 tggacacata ttttggtttt tatcctagtc cacaacttgt tcgtttctcg gttggttggc
    13741 aagagaaaaa aaagagaaaa aaaacataga attcaagaac agcttcacta gcagcctcag
    13801 gaggctctta agtgccagat attaaagcct cactcttacg acatcgccct tgggatagga
    13861 ctccttatag atactcgctt gaaaagaaaa gagctttgaa tcagacatga gccaaatgtc
    13921 cggcggcgcg cccgccttat aacaaagatt tgcaagatac tcttcggtgt cccattttaa
    13981 ctcgactggg acctgtggaa gtaatagtcc cgatccgtcc ctccatgtca atattaatcc
    14041 gtctctgcct acctttatga cattcagata gtccatcggg ttctgtactt tgatctctac
    14101 aggcggagtc agaatactca cctcaaaaac tatgtctaca agttcttttt tgtcaactgg
    14161 ggagaatctc gggtcttgtg tcgccgctgc aatagaagct tcaatgagtg aacggtacag
    14221 gcttttctcc gctaatggaa aaccaatgca accacgcaag tgctcttgtt tgtgactatc
    14281 caaataattc agcgtgacga agactccaca gccttcggta tttcggctga gctcgtaatt
    14341 atcgtcgtta tcattattgg catcgccaac atatgcagta ccgggagagg caaacgcgtt
    14401 aagatgagct tctacgaccc gcctggctag tctgactgcc ttctctccgt cctcgtcgag
    14461 gatcatcatc ggataagagc tagaaatttg gacttaaaaa tcatggtgta atccttatcc
    14521 cttatccctt atccctgcct tccgctgaga ttgactctat tgttgacatt gacgtggtct
    14581 aattgttctg caaatatttg caatgcactt tctcctagcg ttgtgagcaa aaagtggatc
    14641 ggagacccga ggatgcaata ggactttgtc tattgcactg tgagaaagaa ccacacggta
    14701 attatttcaa cttgtatcta attttctcta cataaataat ataaaataat ttatttcaga
    14761 acagaatata gagaagaagt tcattttgcg taaatattat aaataggaac gacggatata
    14821 ttggtgatct aatgtacaaa cataccttcc ggagttcgtc ctctcgtcct tctatttgcc
    14881 caaagtgcgg agaaatagtt aaggccgatg gcgattgcga gtgtaaacga gtccatttgt
    14941 ttcttatctc gtagacataa agaaaaaaaa tgcatatggc aatgactggg attccatttg
    15001 ggaccgacgg ataagctagc tgttcggagc aagcttggcg ttcagcagat tctagacagc
    15061 ctcttgcccc tgaatatata tccacacaat ttgtgacaac gaaatttggg aaataggtta
    15121 aaat
//
LOCUS       DAOR01000264           10608 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_34517, whole genome shotgun sequence.
ACCESSION   DAOR01000264 DAOR01000000
VERSION     DAOR01000264.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_34517"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    8075..8182
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3325 a         2078 c         2132 g         2965 t
ORIGIN      
        1 atagtttata gtacgagaaa ctaaacatgc ataattaatc tgtaattggt caagctcgcg
       61 ggttccaaga tgcttaacca atacgatagt tgataactca cggcgaatac ctcagatgca
      121 agcaagcgat tggcaaccac tgataacttt gctattgaac aaggtaagac gcaaagagcg
      181 atagaatgga tgaactccga ggccgctaaa aacaataaaa agcttcaagt aaaattagaa
      241 ggataccttc tatctacgac aaaattcggg aactttgagg tcgtatcctg gaaaggtgag
      301 tggtctactg cccgtaatat ggtaattagg gcaagtaaaa agctaaatat taaagtggta
      361 gaagctggct accatgtgaa aggtcagctt ctttcttccc tgtttggaat ttccagggaa
      421 tttgcaaaag tatacagtgg cggaaatatt ataggaaatt tagaattaag cacacgatcc
      481 ggtaaatgga tagtgaaagg tgaaaaacta acctagtcta gctaaaatcg agggaaacac
      541 agtataagtt atgaaatata acagcatatg tctgaccaat atgagggaga tgaattattt
      601 ctacaatgag tacgaagcgt gagatttcga tagcagttac agaaaatgaa atatctccag
      661 gtcaagaggt ccatggaaga attgatctca attatctcgg tagatttgat agcatcgtga
      721 tcaattcgca aattgaaaat tcaaatgaca tctttagcta tacgagtctt aacggaagga
      781 gaattaccta cccttatgcc agactttcca tctttaggga ggacataggt aacacgaata
      841 cattagaatt tactgcgacg acacaacata ctccagcaac gaattacacc aacgcaaagt
      901 ttcgagtagc gataatacaa gaacataaag aagtctcaag cgatgttacc tatatcaaga
      961 taacataata attttcttac ttctctgtgc tcccaactga ccgttaaaat ttctgtttca
     1021 gaattgtaac cttgccttgc atccacggat gtggttcaca atggtatgcg tatactccgt
     1081 ccgatgtgaa tacaaatgtg aatttctgtt gtgggggaat tagaccttga ccatggtcaa
     1141 tcgaattaaa tgaaccagag attacatcct tatagttgtt atccgaagtg acagagtgag
     1201 ggactgaatc tgtatttgtc cacacgactc tactagacag tcccaaactg ccttgaactg
     1261 ctttgggcac aaagtttgtg gtctgtgaag gtgaagaaga gcccggtaca attgtaacct
     1321 ggatagtctg tgctggattt aacacttggg gaggaagtac cggtttctga ttagattgag
     1381 gaacatatag aaactgataa tatcccatac ttatcgcgac agcaataaca aacgcaataa
     1441 tactgattcc cgcagtgtga ttttccagaa gactacgttt ttccataaaa aatgatcctt
     1501 ccttcctcta tttgtcaatc gattctaatg atttttcaca tataaagttt gtttgaaata
     1561 ccagaaaaat ccggataatt agcggttagt tatcttatct tttttgtatc ccgggcgttg
     1621 ccattcaagt ccgttgctgt atctggcgca gacgtctcga ttgacgaggg ggttttctgt
     1681 gaccgtccat cgtttgttgc accagcaatg ttggctgtgc ttgaacgtac tggcttcgtg
     1741 gatgtactct catgggctgg aacttgagtt gctgatggcg cgctctttga tgctggcgga
     1801 ggggataacg gtttaccaga agtggaggta gctggtaaac cggctggaat cgggctcggg
     1861 gcagaaggta taggctttgc accggccggc ggagccatag gttttggtgg gggcggaggg
     1921 gttgttgcca tcgtcttggc atgtcgatag atatgaaaca tacccgcaaa cacaagcatt
     1981 atgataccta ctatgaatag tgagaagttc ttcattccac taaggacagc ataatacgcc
     2041 gcgatgttca gataaatttg aaaagccagt aacgctacaa atactatata aatccagttt
     2101 ttagggatgt tgattgaact cttgcctgaa gatttttttg gttgcgattt tgccctaact
     2161 tctgcattct tggctagctt tatcatcata tatgtgaaac caaaggacag gggcaccagc
     2221 atcaacatca ccgagtaaaa gaagatcgga tcaataacta acctttcgag cagagacttt
     2281 tgtatattag gatcaatgta gaatccccag taagtagtaa ccataatctg cgcaatgctg
     2341 gttatcccta ttgcagtaac aagtggccta tctttccatg agaatttcct atacctgtca
     2401 acaaaaggca taactattaa cgtcaatatg agtagaccag gccatgcaac acctgttaca
     2461 aatttatcgt attgggttct aaggaatgcg tacagtcctg ttagatacca ctcaggtacc
     2521 gtaataccag gaggtgacgt gggaacaaat tttgttccta aatctatagg aaatactcct
     2581 ccagttatca ttattgctcc tgctactgcc ataaccattg gtatgtcgaa tacgaggaac
     2641 cttggaaagt gcacaaccat caaaccgagg agtacgatgg ggaggaaaaa cacatggaaa
     2701 gcatagaatc ttaggacaaa atcgtgaaat cccccaccaa agaatgcatc tctaagttgt
     2761 ggacccagaa caggtatgga gttagtcagt gaagccgcga tggatatggc aagctcagag
     2821 cgttcactaa atataatgtc gtagccagta aacgcctcaa gaattgtaag tacgcctatg
     2881 agaacccctg tcacccaaat aatttcattc cttatcttgt atctaccact aaaatattga
     2941 tagtacatgt gaagcagtgc taccataacc atggcgttag atgcatgata gtgtatattt
     3001 cgtatatgga acccataggg aattgtgttg ttaatatttt gcacgctatt ccacgctcta
     3061 tcaagctccg gttcgtacca aagcattaac agggctcccg taatacccag aattatgaat
     3121 aggacaaaag taatcatgcc aaggaaacca agtgggctaa cgaatcttcc aggaaaggta
     3181 aacttgagcc ccatgaagat agttctatct atgccggcat aaatccactt aaggaaagtg
     3241 actagactgt tttcatccgt tattgtggca accaaatttt cttacacctt taggaatcga
     3301 ccgaagccca caactccgtt tccatttgga ctccagttgg gaggcaaaat ccatatgttt
     3361 ccatttccat ctatttctag atctaacatc ggaaggacat tactgggtgg tgattgtacc
     3421 gatgctggtc cgacaaatgc cttaccatta agcggatcat acatactacc gtgacatggg
     3481 cattcgcctc gctttctgcc agggtcaggc cagtatttcc atagacacca aagatgcaag
     3541 catatcatac tatacattct aaaggctgac acgtcatttt ttttgccccc tagctccagc
     3601 ggcaatcgta taaattgcca aagtcggaag gattcttggt ttaatgtttg atcactagtt
     3661 tttggataaa caactgtttc agaatgatta actggaaaag tcttaatatt ggcctgcgaa
     3721 ccatcaggca gctcgacttg ggccttggca gatgtattgc ctgcaggatt gggtaagaac
     3781 ttaccccaat ctataaaggg tatgaaagtc ataaccgtac ctgcagcggc cataagcttc
     3841 agaaaatcac gccgcgatac cttccttcca gaattaggag ggctttgctg cgtcatcgtg
     3901 tacagaaagc agcttcaccc atatctataa acttttggac atttattaat aagcgcttct
     3961 tggctactat gtgctatcta accggagcac accagtgacg tgaagtatca atcacgattg
     4021 tattacagac tatcccataa tgacaacgtg caatgggagc tccgaatgat tgctaatcca
     4081 gcactatttt ccggaatatt atccgcgttc tgtttcctcg ttgtttattc atccaatcta
     4141 tctcttgttg ctatgacgcg gttatccaca ttcatcgatt ttgtctcatg ctttgaagat
     4201 tctgctaagc ataatcaact cagggccgct tgtcaaagat cctacaatca cggattttga
     4261 attcgccagt attccagaag atagaaacgg gatccctccg ttaacggtaa caggttcaac
     4321 ttggacttgc aatatctcgg aaatcacctt aatctcgtct tctgttgctt ttgggtgaac
     4381 cgcggcgcca gtatttgttg cgacgatcat agaacctgtt tgaatgaagc cgcctaccgt
     4441 catcgaatgg acaggaacac ccaaagcgtc gcgaatttgc tcgtctacct cacctttgaa
     4501 tattggagat actaatgctc cattatcatt ggttgaaatc aaatttccaa tggcagtaaa
     4561 tttgctattc agacgatcaa cattaagacc ggttgcctgc ttcagaacaa taatctcttc
     4621 atctgtggca atagagggaa ccaaaattcc cttgttattc attgccgtca tcggaccgat
     4681 aagccttgtg ttcgcaacag atgtataaag ctcctctttc acgcctaggt attccattag
     4741 tttagcggtt ttagtttttg caaaaccaaa aggaactatt attattctat cattggcctt
     4801 cgcaaataat ccaagattag ggcttttata tacgccgtat ctataaattc ccaagtattg
     4861 aattttctgt gtaaaaatat gaatatgaac ctattgaatt tagactgcaa aatgaaattt
     4921 atttctccta aaatcaaatc gtcaaatctt gtttcccatt tccattagtc catgaaatcg
     4981 ctctttaaat aatttatccg tctcttctaa caaagaactc ggtgctggaa tagagaataa
     5041 aggataaaat ccaccacaca agaaacaaga tgcacgagat tcgcaacaga gcatattact
     5101 gatagcctct agaccgaaat agagacactg aattgggtgt tgaacgaaat attggtagta
     5161 tgctgctagc cgtagactta tttggtttca ctcggacacg ggaaaaactc aattatcaat
     5221 actttagcgt aaaagactta taccaagtcg acccctaaaa ttgtgtcaaa tttgattcac
     5281 ataccgacga taggcatcac cagtagaaat aaaaacgtag tagcaaaaca taagatatca
     5341 atccaaattt gaacgtaaat aaatgctgcg aacaaaatgc aagtcattta ccgtaccatt
     5401 ttggggtctg taatcttcat tatctgaact ttttttatct atggggtgaa tgaagccaag
     5461 ggatatgcct attaatcatt tttttagtcg agacatagtt tcaatcaaag atttcagcag
     5521 gcaggacctt gagtttattt tcggttgtac agatagagtt agcgcgctaa gaccaaacga
     5581 aaaggccgaa ttgggcaaag gacgaactct gggttatatt ttctacgagc ctagcacgag
     5641 aacaaggatg agtttcgaag cagctatggc atcgttggga gggagctcta tcggaatttt
     5701 tgattcgaaa tcctcgtcgg tcgaaaaagg cgaaagcctt gctgatacta tcagaataat
     5761 ggatctttat tcggatgtta tcgttctcag acatccgcta gatggctcaa gcagattcgc
     5821 cgcagagcta tcaaagaacc cgctaattaa tgcaggcagc ggaagtgaag aacatcctac
     5881 tcaagctatg cttgatctat ataccatact caaagagaaa aacaagattg atggcctcag
     5941 aatcggaata gttggtgatt taaaatatgg aaggacagtt tattcgctgt tatacggcct
     6001 cgcaaattat aatgtaaata ttcacctaat atcgcctcca acgttgagca ttcgaaaaga
     6061 gtcaatatat gatgttgaga agaaagaatt gaaaattttt gaacatgaag agctagacga
     6121 caatattttg actaaacttg atgtaatcta tgtcacacga atacagaaag agagattccc
     6181 agatccacag gagtatgaaa aagtaaaggg cacgtatact gttgatagca atacgcttcg
     6241 caaagcaaag ccagacttat ctgtaatgca tccaatgcct agaatgcaag aaatttcaca
     6301 ttcgattgat aatacaaaaa atgcaattta tttcaaacaa gctgcctatg gaaaggagtt
     6361 aagggctgct ctcctagccc tagtactaaa tgaaaacccg atataagctt gtaaataagc
     6421 gtcggttatg aaggacacac tcttttactt tttcaagatt ctttcgaaaa cgactggctg
     6481 gttgtcgcat caatagttca agaacgaggc tgtaatgtca gaccgccaga cctgtttggt
     6541 gtattgtcct ctttatatat ccgaactaat tgagacgctt tgcgggttat cgcctaaatg
     6601 tttcaattgt attagcgtag gttgacgatg agcgtatagc attcctatat tctgtaagtc
     6661 aggggatgtg cttagaccaa acgggtgata tagtcacaat gataagctat ggacataata
     6721 taaaatatca aaagtatgat gagttatcta taactctgac catattctta acatcaaaaa
     6781 ccatcacctt cgttggtaat ttaagggtca agagtcgatc ggggggaggg ttatacttta
     6841 gaacgtggac aagtccgcta agccttttcc catggcgttt ttcgataaca atatgcgggt
     6901 cgaacaaggc atcgaatgtg aaaaaatatg tcgcgcccca tacatttaca gaatattcta
     6961 ccctataata aggaattgac attctaggaa acattagatt acctgatcca tgaacgtttt
     7021 caccttggtc atacgtcctc ctatgaaagt catcaatgaa tggaacctta gtgaacactg
     7081 ttgacctaac tgatttcctg atgcaaatga tcacatagcg attaaattct tcgtcaggca
     7141 aaattgcagg accatatctg gcatcatcat gccaacttat tctgcgattg accaaaaatt
     7201 tttgcgaagt ttcgattagt ctccgaatat tctctacaaa ttgatgttca ttacattcgt
     7261 aaaccggcat agtgttgtaa ttcctctgtg attctcgaaa cggtgtgagt tgatccacaa
     7321 attgtaatga tagggtcttt tggataagca tcgccaatct cctcagagac actgattgtt
     7381 tcttccccat taacatagag catcctcttc cagaatttac cctctgcgac aattgtcgtg
     7441 ataccatcta gtgaaaaatc aagttgagaa atactagctg atacttcttg caacggtata
     7501 agtacagaaa tcgattttaa tttctgttta ttttgcatat ctctgtatat tagcaacgat
     7561 tcacgaatat aaaaataggc ggcatgctgc atgagaagtc tccatcacgt cgctacaact
     7621 gaccaagggg gaacacacca cagctgagaa acgtgtcttt ccaaggtgac ttaaggtagc
     7681 atcagaagaa tacgacatta tagtgtgatt acaaggagtt aaattgatat tgattaatat
     7741 cgtcaatcaa taaattcatc atgccttcgc cattgcaaaa aaaatctaaa gttaaactat
     7801 gttgggcatc aggtaatgaa tataaaaaaa ctaatggcat caaagaaccc attagaatct
     7861 tgaagaattc tacaagcggg aacttgctct tccactctga caatcttaca tcggtgaatt
     7921 atctactcga aaatggcttt gaacaaaaaa ttgatcttgt ttacattgat cctccttttg
     7981 ccaggggcga aaagtattat catcgtatga aaaatgtcga aaatcctgcg tttgaagatg
     8041 ttttcaaagg aaaaatcgat gagtacctcg atatnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nngatgagta cctcgatatg atatacccaa gacttgaagt
     8221 cattcaaaaa ttaatttctg ccagggggtc aatctttgtc catcttgatt ggcatctatt
     8281 gcattatgtt aaagtaatca tggatgaaat ttttgggtat gaaaattttc gtaacgaaat
     8341 cataataaaa agggggcgaa ggaaaaatct tctctatcag ttcaagtcaa tcgatagaat
     8401 gcatgttgca aacgattcaa ttctttggta ttcgaaatct gcaggaacga aatttcaaca
     8461 cccacttgtt aaaaataaga ggaactccaa atggatggga ttttggagta acgtaaatag
     8521 accgacaatg cgatacgagc tgtttggttt tgtaccagag agaggacaat ggaagtggtc
     8581 caaggcaaga gcactacttg caatagaaaa ttacaaagtc tacgtaggca aattcgctga
     8641 tatccccctc gaggaatact ggagacgagc tagtcaaaat atggaattta ttcgcaaacg
     8701 cccgggcgtc aagtatccag aatactggat ccccccgaag acccatgtaa ttctcgacaa
     8761 tatctggacc gacatagaag cgtacagtta ttcaacaggg tatgacaccg aaaaacatga
     8821 acaattatta gaaaggataa taggacaatt ttcgagggca agagatcttg ttgcagactt
     8881 tttctgcggc tcaggaacca ccttagtcgt agcacaaaga ttgggcagaa attggattgg
     8941 ctgtgactgc tcctcgacag caatccaggt aacagaaagc agattagagc ggtcagattt
     9001 tagtctaatc aaagcattga tgccgagggg aaaatattgc cgtggaccag tatcttgagc
     9061 caagaagatg catagataag actggatgcg aaatcgacaa tcagatgctg ttccattacg
     9121 cataacgcac ataaatccgg aaaatataac tccatttgtg acagtccaaa caaggtcaga
     9181 ttatgaaagt ttgctcaagc ggctacaaga caaactggga aacgaatcac gaaaagcagt
     9241 ttcaagattg gaagttccaa ctccccaaat tatctgggta ggtcaacgta caatattcag
     9301 aaactttatg gagttcccaa aagccttgcg aagagagcca ccgaagttac tcctgtatct
     9361 taacaaggag cttgcttctg ctggctatat cgcaggtgaa cgagtaatat ttcttggacg
     9421 aaaggaacca tcgtcctttg gcgcgctaat tgataggtac gtaaaggatt atgtaatatg
     9481 tcctgtctgt ggaagtcctg atacacgaac cgaaaagaac aaaaagttag ggtttctctt
     9541 gtgtgaagct tgcggtgcaa gatcctctat taaagcaaac tacgcttgac agaatctctg
     9601 ttatacttac cttcttatac cgctcagctt cttggcattg cgaattacgt ccatcatgtg
     9661 ttttagttga aaatccccga ctaacgagga atctttttac aatatgtctc gtagttcttt
     9721 gggctgctcg ctctcttcta tgctttgtat tcacaaaaag gaataaacaa atgagctgat
     9781 atataacgag aaaatgtcaa tcccagatac ggccaaataa tctatcaggg gcttcagtag
     9841 ggatcatctg gccctcttct catgcattcc aattatttta cgctgggtgt ctatactttt
     9901 tttccagcct cttgatcaag agcagtaaat aaaaataagc cacgattatc atagattaaa
     9961 ccgaacgata agtgatagtg aatctcaatg tccttatact ttggtcgata tgcaaataat
    10021 tctattagaa tgacactttt cttgagcctt tatcccgtgt tcggttacag agatatcctg
    10081 ctttcgctta agtggctaat aagtactaac gcgatccatg tggcggccca tttctcattt
    10141 tatgaaataa tttaagtgta cgcaacagct cattttcaaa gaaaacatat actataaatt
    10201 gacaacttta gaattgacaa tctgcagata atatccaatt ttaacataac ttatgaaatt
    10261 cttatacgaa tgcgattgtg tccagtgttt ttactacgag agcggataaa gctaactatg
    10321 ggtcctaagt accgcacatg ttattatctc aaaagatatg actgcaataa tgtcgcttaa
    10381 tccagaagca actacacaac agagccaaaa caatttctca actggtcaaa gttggggagc
    10441 acttagaaaa gcgtggaaag gttatagaat tgctaaggtt cagggtgaca acgtcaaaat
    10501 gacagaatat gctaccaaaa tcagaaaaat ccaaggagaa ttaggaatta gtgtggcatc
    10561 gtttccaaat ttaggtatat cctaggcctt gaggcaactc aaacacac
//
LOCUS       DAOR01000265            5651 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21929, whole genome shotgun sequence.
ACCESSION   DAOR01000265 DAOR01000000
VERSION     DAOR01000265.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21929"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4775..4896
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5223..5492
                     /estimated_length=270
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1639 a         1059 c          930 g         1631 t
ORIGIN      
        1 tgcagctgct cctggaatga ataatactgc tggaacgact gctttatcgg tacaacgtca
       61 atgtgtgagc aatatactag actcgcccac catgcccgcc gctcatcaac aactcatcga
      121 caaaatagtg gcgtgcttta atcaaaaatg aataatgttg gattaggtat aatatagcga
      181 aagaacaatg ttatggtcgt cgccacgacc actaccttgt gaagaaaatc ggcgaaattg
      241 aagagtgtgg gctcagcacc tatgtctgat taagctgatt caagacttca tagaagttac
      301 tggagactat ccaggagctg gtgttggcac atcacatgat gtgagaggtt acgagcatac
      361 atctacatca tcatttagat catcctaaat aggctacacc aatatattaa attaaataaa
      421 ccgccattgt tctcttcacg aacttgcaat ggtatacagg ttttacaccc aacgatgttt
      481 acgcaaatcg agtcgttgat tcatccacca agctgcttta gtatcctttc tattaccttg
      541 ttatgttcag catccacctc ggttgttact aagagcaaat ggtcaggatc taatggaaat
      601 gttatacgtt taattttttc atattcagcc atagcatatt ttccccttcc taccttggga
      661 gaaagcgaat tgcgtaatcc ccatctagcc attgcttgga ggtttgattt tttagtttca
      721 tccggagtta gcagattctt tatcccttca cgttgaccgc catatttgat ttcgcctgtt
      781 tcatcgcaga cgccagcgaa tcggaccttt gcgtcaagac ccagtatgtc cttacataat
      841 tggttatatt ccatggtttt ccactaatat aataccaggc gaatctaata aagatttgat
      901 tttgggagtt ctacccaact tactatctct ccgtgaggca ctactagcat aggtgggtac
      961 gatggaacag acagatatat ccaatagata atgcgtttgg atattgtatg gcttcgatct
     1021 gctaatattt catgctcaac aagggagcca tagtttgacc ttgtttcaaa ttcttcttgt
     1081 tttttctatt atatgtcaaa taggggttct cgacatggtt tgaaaaacct aaaggcaatg
     1141 gttggcccat ggactctaag ttgctgcggc atcggttctt gctcgagcat aattctaaaa
     1201 cgttgactcg gaattcagca ataagtgaac tcagtgcaac aataattaat tattcggata
     1261 gattcatttt agaattgatt gctccatcta atattataat tattcatcac aaaccagatg
     1321 tagactaatc ataagattag ttataatatt gttcacctca cttcatataa aatatatttt
     1381 gagtaaagaa agtgatgtat acaaaaacat cggctaccta tgtcattgta gtattggcaa
     1441 ctatagcatt agtgcttatt acagcagcta gtttcagtac tatttctgct ttagcttgga
     1501 accatggaag aaaaatcagc gacagtcaaa ataatattgg cacatcatct aggacatcta
     1561 acgcagactt gaaaagcctc tttacctgta tatctaatgg agccaacggt ctaaatggac
     1621 ttactcagaa caaagttctg aattgttata cacagacctt gaccacaagc aatacaacga
     1681 tatctggtat tagcagttcc gtaggcccca atttaagtaa tggcaacaat aacccgattg
     1741 agactagtga tagtgggtag aactgactca ccacaagagc atagtagctg tacctcctct
     1801 gctgacaata gatgataata atgattagca tcacagcctt taagaatcaa atccactccc
     1861 tcaaaccaac gttgtgttac agtagccgcc tatataaaat ttagccttgt ctaatatgat
     1921 taactatcta cgagataagc gatcgtcatg ctatgaatgt ctcaatatgg tattcaatat
     1981 cccactagca caaaagataa atgtgcaact cgattcatga tcactcttaa tgaattaagt
     2041 tgaaagccag atcaggggcc gaaaatgatg accgtctcaa ggtcattcgg gatgttcaca
     2101 acacaaaaag agatgcagct caagatcaac atactgacag aatggaggac tacttagagg
     2161 tcatttatga actcataaaa cagaaaggat atgctacggc tgtagacatt tctgaatctc
     2221 tcaatgttag ttctccaagt gtaactaaaa tgcttcagcg actcgatgaa agcaaatatc
     2281 tgcggtacga gaaataccga ggaattaatc taacaaatga gggaatttct gttgctgaaa
     2341 atattcgcga gaaacactac ttattagctg aatttttcaa aatgataggg gtggatgaaa
     2401 atactgctaa tattgacgct gaaggcatag agcatcacct tcatcctgag acattgaaaa
     2461 agcttcagca atttataaaa ggctttaaaa agtaaaagag tcttagtggt tccatcccca
     2521 ataatggatt aaaatctttg atagatcact gacatcgagt aaatgatatg cgctaattaa
     2581 tagcagagcc ttgagctcat tctacgctgt agaacaagag caattgctca agtaatttta
     2641 atcgaatcat tcacacaaag aacatctgcc attatgaaat ggcttagtac agatcttctc
     2701 tctacagttg tataatatcc cacatcttgt gcattgatgt aaaggcaaat attctccctc
     2761 ggcatgcatt ggcagatgac catagtatat agcatggcat tcatagtgtc aatgaatact
     2821 attgaggatt ttcaatatat tttcaattag ttcaaggtga ggtacatcat tctccataga
     2881 aacacgtaat aggtactcat cgagtgggaa agatgcaagc tttattctct ctctttctga
     2941 aaaactatag atggttctac caaattccga gtcaaaatct ttactcatct catttctgag
     3001 cgcaaattcc aaatacaatc tcgatgaatc ctctctatgc tccatcgatt gcatcccttt
     3061 tctcatccct ccggcgacaa gttttcccat acgacttata atacctacaa atcgaatctg
     3121 aggacttagt ccaaaaatgt catcacacag tttttcaaat tgatgatgtt gggaactcat
     3181 aaattatgtt ctctacttag catttgaaaa gtacttctat ttgagtgatc tcattttatg
     3241 gaatactatt accaccccac acatgtttat ttttttcatc aatttgacaa ttccgacgct
     3301 taacccgata gggatatgca ctagtgaagt attcacctcg taagaacttc gtttatttaa
     3361 gttgtttgct acgggcttct gttgttgagg ctagataggc ctgtttgaaa agatatacgc
     3421 taagggaaat ttatcttcaa atcttgacaa ttttaccaaa tcataaagtt tctccctttt
     3481 ggaagccaaa ataacaagaa tggcacattt cttttccgta aataacacca ttcttgccac
     3541 atttactgca tcccctttca tagtcgacag ttgaatctaa ggacttggct ctttgcatgc
     3601 acttaccgca aatcggcgct tcaagtttga agctagaaag aaaaccagac ccgcactgaa
     3661 gacactttag actatacatt tgcccctttg gtttccaacg tctgaaaatg ttaatcatga
     3721 tccaccttgt agaccttgga accacggcta taacatgcag cctggttatg ttttattcca
     3781 catctcatac aaatatttca atgtacctcc ataaaatggt accggactga tatttctcat
     3841 ttgattctta gataaggcca acacgggtct tttcttgtat ctcataaatc tcggattgtc
     3901 attttagctt tgaacacgta cctcgtcgcg aagaacatag atagaatatc gaaataattc
     3961 caagaacctt gacacgattt gttagaataa catagacact ttcttgttag caaaggaaag
     4021 gaagctgcat gcttctaagc gtcttctctc atttgggaaa atcttgcaag agtatacggt
     4081 actaatgact aaagtaaatt ctacctaaaa ataggaatac atactccgtc gttctatcta
     4141 acttatcttc atgggaaagg aagcaataga ggtgtgtctg tttgagaatt atgattattg
     4201 gaatttgttt gcttgctgct atcacttttc tgcttgacat cgggtgaatt ggtagacatt
     4261 gagacatcgt ttgaacccac attcgtgcaa tgcgtactat tacctttaca ctcattatgt
     4321 tgactgatct tttgttgata gtttttcttc tcgtccatag cgattgatct ctgtatattc
     4381 aattcgttta agactgtgaa ttggagtatc atagaaatag aaactaggcc aatgatcatg
     4441 acagcagaga atatcaatac ttcatttaac gtcattgtca ttaggtatat ttgaattgcg
     4501 gatttttatt ccatatttta atgctcatag aatatatgag aactattgtc acattctact
     4561 ataggtaatc tcaatacacc cggaaaataa aagatataac atctggcata tcttgtcgtt
     4621 caacagcaca attgccgcca ctatcggagc catcggccgt ggattaccga ttttcataca
     4681 cctgcgctaa taaaatcgac atctgattcc tcctgtaggt gcgatttctg ttgagaaata
     4741 ttcttcttat tgacgtatat ggtatattgg tatannnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtat attggtatat tgatatacgt
     4921 tattactgta atactttttg ctatggtttc atgttctatt gtaatattca agagacagat
     4981 tcgaaaatgg atatatagac agaaaaaata ctgacaatag gatacgtctt ttgataaata
     5041 ataaacgaat agcaataagg acaagcctgc tcacattgtt tgtagctgtc gcactcatct
     5101 cctcagtaat cgttatctcc cagacacaaa atgaattatt agcaaagaaa accaatggaa
     5161 ataaatcaag ctccggtcaa tcaacctcca actctacatc ctcaccacct tcatcttcat
     5221 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaccttca tcttcatcac catcaggtat
     5521 tacaggaggc agtagcatta gcagcggcac tgcttctgat cttaacaacg cccctggttc
     5581 tggagatccg acacattgcg atcaaccagg atatccatct tgttatagtg taggttattc
     5641 agatggactg a
//
LOCUS       DAOR01000266            4707 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29249, whole genome shotgun sequence.
ACCESSION   DAOR01000266 DAOR01000000
VERSION     DAOR01000266.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29249"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3136..3351
                     /estimated_length=216
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1355 a          843 c          884 g         1409 t
ORIGIN      
        1 atttcgaacg gtaatggcga agacaaaatg gtcctcgaac gtcttattcg tggctttgat
       61 tctccccgta gcgacgccga cgagcagaac ccggtctccc tgccctacga attcgggggg
      121 ctctgggtat gaagtttcca ccgtttcgtt agccttctga agtaaattct ccattcctgc
      181 gtgcccgcgg tgcgtgccgg ccagcggcca gtccttgccc ggaacgatcc actcaatatc
      241 ttcggcagac aacgccagca aaccttgctt atcgcggcgg ccgagtgctg caaaaaaatc
      301 tttcacaacc tggacattct cttgtatgct caaaaggaaa tttgtacttc gtgggtctac
      361 attcatctgc gctgtggttc ggaaacgtta ctcaaatgaa gaataatact tcagatggac
      421 tagcaaagat tccaatgcca acaaatggat tcaatggtgt aagctctgct aagattagta
      481 ctggttcttt agcagttgat aataaaagga atgtggcatg ggtctccatg ttatctattg
      541 cttcggaagg agagattcta cgctataata tgacctcaaa aacttttgct acttttattc
      601 ttcccgaaca gttaagctct cctgtaggtc ttgcagttga caataacggc aatttatggg
      661 caactgacca tggcaccagc atattttaca tgttagacac cacaagccac aatgtaacaa
      721 tgtttgcgac gtctaaagct tcaccaaaga tatatggtct aaatgattct actagtcttc
      781 cagaaggagc taatacactc ccatattgga tacaaaaagg agctgatgat ggatcgcttt
      841 ggttcaacga gcatcagggt aacaagatag ccagatttga tcctttaaac aatactctat
      901 atgaatattg gattccaaca caggataggt tatggggcga ttgtccgcct agcagtaaga
      961 catgtgggat agcaaatgtt ctgcagtttt caagaggagc gaatggccag acatggttcg
     1021 ctgaattgtc agaaaataaa ataggaagca tagtaccaac aacaaataat tataatcatc
     1081 agtcaccctt ttctgtttca gcttcatctc aagagctaac aataaaaaga ggtgagagcg
     1141 tggaaattaa ggttaatatt tcgacaactg gcgcttctcc gtcaagcagc aatattgata
     1201 tggtatcatc aggaacattt actcccacgg gcgatcttgg taattccaca ggatccttta
     1261 gtgaacaatc tttttcacta gaaccaatgc atacaaagga ggtttccttc atcttcaccc
     1321 ctgcgacaga tttggggtca ggagaatata cgttaatgct cggtgcacaa aatgacgcga
     1381 taacagttat gaaggcagtc aaagtacata ttcttgatta agaggatcaa tctatttaat
     1441 aatttgtctt ttttacttgt attaaataac ttgaattgtc ctgttatcgt tgcaagacag
     1501 tttgctgtcc ctggtgtaca tcgtctgaaa tactgattaa tgaagattac ctaaatccag
     1561 actacaacaa aaatatgcga catactacgc tgcacaatag tggtatgtta tatttggcga
     1621 tagttatagt ttttattttt tagttgatct ataatcactt taatcagaaa tggaaagcta
     1681 cagacatttt agaaactttt tagaaaatgg tagtactata gaggatgaga aatcaacaca
     1741 actcttatct gatgcattag gatatgagtt caactcattt gctgcttaca ataatcagac
     1801 caacaaccaa acaccttttg tctgaagcga aatcttgcag gaataataat attgatacta
     1861 tatctcaaac taaatggttg aaattttgcg agattcatgt ggtttaaact tgtcccgcac
     1921 catagtgcta tagatcaaat cccccatgga gtaatctcaa acttagttaa aagaaagagg
     1981 ataagcgtaa gtaacatatt gtagaaaaat aaaaaaattg gcggattttt ttggttgatc
     2041 actgctgctg aatgaaatgt tatttttcta atgcacgtcc taatcttagt gtttgggacc
     2101 tgttgtggtt gtgggtggtg gagatgttgt actttaggca ttgcagaaga tgaggacgtg
     2161 gtgttaccgg cagtcggatt accaccagga gtagcagcct gcacatgtat tattcctttc
     2221 attgtatctg gatgataaag gcaatgatat gttgagatgc tgtaggaaaa aggaacatga
     2281 aatttaaccc cttgcttcat ttcgttcgtc aagacattga tattgttatc atattattat
     2341 aaccgtttta gcactacttc ttaatcactt cacaaaaaga tacctaaaga ttgattttgt
     2401 tacggttccg tcagttgcgc ttattcccag agtatagtta ccaggagata tgggagtaga
     2461 gtcatttctt tgcaatataa tattagtctg tgctttccca cttctatttt tgttcaaatc
     2521 aataatagcg gtaggataga actttgcagt catgtttaca aatttgccaa gcggattcat
     2581 tgaacttgat gcgttgagaa aaataagagt gcgattgtta ttgttattag tattagtaat
     2641 gaaagaagaa ctgttaaatg attttttagt agtaacttcc acttttaatg tcgctgccac
     2701 cggtgatgca tttttctgtg ctgtgttatt tgatgatgat agaatggtaa tcttatttgc
     2761 tgatgaattt atgtcaaatg ggataggtga actcctgtct aataccccaa tcttatcatg
     2821 attaaactca gtaaaccaca ccttctttat gtcgttggga tcagctgata gagtaagcgc
     2881 atttgctatg tacccattaa gtggatctcg tgttggtact tcatactcta atagcgccat
     2941 ggcagtagta ttgaaaaagg ctattctgtt gccttcatgc tcattgaacc aaaaaccttt
     3001 gtggtctgct ggttccgcga tccaataagg taacgtgatg taatgacgag ggctactaga
     3061 tgtcggaaat tttatgacac tgtgagaatc tatacgatat tctgttataa agctggtacc
     3121 atgttcagca agccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaaaaagtt
     3361 gtttttggcg gctagaagaa gagggtgacg atgatggtat ttctaaaact tttgtaacat
     3421 taacaatctt tccgttagga tcgactattg gcttaaattc aactaattta ttatgattta
     3481 acgtctctgt aatccatact gaatctcctt gtagaaatat cccgcttgga aaagaatcat
     3541 tacctaagtt aaattcagtt actttgtatt ctggacttgt aatactatcg ttaagattgc
     3601 ttttgatacc agccttgttt ccaattatct tctgtataac tccaaacgtt ccactaccaa
     3661 atgtagtaaa ccatatattt cctgatcttt ggtcaacctt catttgcata ggcgctgcgc
     3721 ttatggaatg aataacctga aatttctccg tattagggtc aaagcgccaa agcggatctg
     3781 agcccccttc ggggaaccat attgaaccat cagtaccttc tacaatagac cataccatct
     3841 ggaatccctg tttggactgg gggtttgtat tgttagcctt ttcttcagga gtgatgatgg
     3901 gatatgatga tcttatctta ccttgttttg gatcaaaacc aatcagggta tgagacatcg
     3961 ttccaactgt ccaaattatt ccatttctag ctaccagtat agcattaggc caagtaccct
     4021 taggcataga gtactctttt acgaacggtg tatcaatatt ggtagaatta ttgagaaaga
     4081 cttgatgtat cgttgtatta ggtttttcgt tattgcctgc tgggaaacct ggtcgcgatg
     4141 aaatagaaaa acccactccc actattacga ttattccaat tattacagat atagttgcaa
     4201 cgagtctcac agaaggcatt tcgtctcttc actcatcttt ctatcttcca gtaatcatca
     4261 tgttattcaa ccctattctg cgaaaaagta ccacatgctg taccaattat atcagatatt
     4321 ttaactaggt agtttacaaa tgtaaatcaa aacttgcatg agtggaatat caatttgata
     4381 gaccttcact tttgccattg aaattgcatc attatattca ctcactcata acgattaata
     4441 caaggtatat tgtttattga catggaaccc ttgtttggac actaaacttg acaacacaga
     4501 cattgcagta atcaaatcat tgatacagga tggtagaaag tcgtttagac agatagcaag
     4561 agagattaaa gtaagtacgc ctaccgtaga agcaagattt agtagaatga aggggttggg
     4621 gattatcaaa aatatacagc ctattttcga catagaaaaa atagatagcc agatggctgc
     4681 tcttattttt ataaagacaa acccctt
//
LOCUS       DAOR01000267            5101 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20137, whole genome shotgun sequence.
ACCESSION   DAOR01000267 DAOR01000000
VERSION     DAOR01000267.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20137"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3535..3761
                     /estimated_length=227
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3927..4248
                     /estimated_length=322
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4600..4850
                     /estimated_length=251
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1191 a          935 c          809 g         1366 t
ORIGIN      
        1 aatatgatag ttcaatgcta ttgtggtctg aacctcttgc aagtcattag atgcggctgt
       61 cgcgtcggcc tggaatttca aagtacgtac ttcaagttgc actacctgtt cagcaaatgg
      121 gacgatgaaa tggattcctt ctcctaatac acggttttca actgcaccca catacagaac
      181 aactcctcta tgtcctgctt ctactactac tactgattcg aacataaaga caacgataac
      241 aacaaatgtt attattgcag ccaagatttt caacttgttt tgtactgttg gaatccttgt
      301 cattggtatt accttcacat gccggtttat taactgaata gcgtaccctg tgcagtgtga
      361 gtagctagct atcggtgatg tgaggccgtc acgctttgtg ttctgagttt atttattgga
      421 tatttataga aaaaacaaga gagagaaaga aaatatgctt ttctcaatct ctttttcaaa
      481 tgccgtcttc tatattaccc tctggttgat ctgatgtaac atttgcagat tcagccccat
      541 attttttctc gaaaagtcca ttttttgttg agcaggaaat tgctaactga agcagctgca
      601 actgccaaaa ctaacgacgc tgtgtattgg aaaccagatt gaacgaacaa atatagaagt
      661 attaattgaa ccactcctcc tacagtactc aggccgagaa acatgccgta ttgtttcaga
      721 gtattcctcg gcgaaaagtt tctgtcctcg aatgtccaaa ttttgttcag aaaaaagttg
      781 gaggttattg ataagattat cccgattgta gaggcttgca aataccaaaa attcgagatg
      841 atgcccttcg tcaacatcat tgacacaata tagtttaaca ataagcctgt ggcacccaca
      901 gaataaaatc tagctgcctt ggatagaaac aatacggatc tgcgtttctc ttctttcgct
      961 gttactagtt ttgactctcg gccatatctg tatagatgcc acactgcttt gagataatca
     1021 agcatcacgc ggttatcaaa cttactttct cctgattttc tatcgatgaa agtatatggt
     1081 atttccttta ctcttactcc ttgggcctta accaagattt caaggagcaa cttatatccg
     1141 tacgtgtcga acctaatatt ttcgatgata tgacgcggaa atgcaaaaaa gccagatata
     1201 ggatccctaa cattacacaa cttcagtcca tgtattgcaa tctttgccgc caacctgctc
     1261 aacagtagtc tcttatacgg ccatcctcgt attgatccgc ctcttatata tctcgaggca
     1321 actactatgc tgttgggatc acgcaataac tcattgatta aaagcggtac ggtttctggt
     1381 ggatgagaaa aatctgcgtc cataaccaag atatacttac ccgtggaaga ttttattccc
     1441 tgtaagattg cagagataag cccacttttg ttctctctac gaactagcct aactatggat
     1501 tctctggtat taattgcaaa attgctacta ttttcatgcg cttgttgaat ccccgaacat
     1561 accttagtaa gggcattctc gatataattt gcgatcaact tgccagtgcc gtctggggaa
     1621 ttgtcgtcta caataattat ctctgtaaaa aggccatctg gaagcttatc ttttataatc
     1681 tcgactaatt gtagaatatt ttcatactca ttatatgtgg gaattattac agagagatcg
     1741 gctttttcaa acaaatttct actattatca tggtctaatc aacttaaatg ttatgataga
     1801 tgacaatgag gcgagtatga cacgcacctc aaattccagg taagaattct cctagccgtt
     1861 agctgcatgt cgtaatagca tagggtgtgc atcaattcaa gtcttcagtt gcttacgttt
     1921 caaacgcgta aaacacgctg gttcaaattg tttcatagta cctaacacaa gaacagtgag
     1981 ggatgtagga ggcatactga tacgctttct tcacttagca tgcgtcccaa agctacgata
     2041 tgtcatcgtc tgcatttttt tagcagcaaa ctgattccgt gtattcagaa gttttgggct
     2101 gattttggtg acaattgtta aatttgcagg gctcgcctgt taacttccag aatggttaaa
     2161 cacttattgt ttctcttaac atcctcaagt aactcttcaa tctttttcag tattgcacag
     2221 atatggcacc gcctaggata cagtatcgac taaatgtggt gtattatcac attttgcccc
     2281 tcacgcgagc agagtcatgc atatcttctc tctaattcca tgtatttctg caaatctaca
     2341 agtttattaa attcatcgaa tgtaaccatc attccgcgtc catgttctgt cgagccagtt
     2401 ttctttaatt cactgaatac tttcattata gcgaaagtcg cagcataaag agcagataat
     2461 ggaaacaggg caatgttgta ccccatgctg aatagctgct gagcgctaag agttggtgtt
     2521 accccttcct caatcatgtt tgctaccagt ggcgcatcaa tttcatttgc gatcttggtt
     2581 aattccttag tcgacgttgg ggcttcgata aagatcacat ctgcaccggc cttgaagtac
     2641 gttttgcccc tattgatggc ttcatctaag cctagagagg cacatgcatc agttcgggca
     2701 acaacaatga aatcctggct tttacgcgca tctaaggctg ctttaagttt caaaaggtac
     2761 tcgtccttcg gaatgatact cttaccttgc atatgaccac atctttttgg ccaaacttga
     2821 tcctctaaaa atataccggc tgcacccatg ctttccagat ccctcactag tcgccaaaca
     2881 cttaatggat tcccgtatcc cgtgtcgacg tctacaatta cagggcgatc aacggcacgt
     2941 attattctcc gtgcattatc tacggtttca cctgcgttta aaaatccaaa atccggcatt
     3001 ccgagcaagg acgcagctga accatagccc gtttggaaca tcgcatcaaa tcccacatgc
     3061 tgtgctatcc tggcggtaag tgcatcgaaa actccaggca taactataat gctatcgcgt
     3121 gacttgattt gctggcgcag agaactctta ttttttgttc ttaccatgtc attacgtatt
     3181 tcagttgctc gaaaatatta aagctagtgc tgttgtttgt aagcacgatc acctattctc
     3241 aatataattg gcaatcgaat ttttttttgt ctaacatcga taaattctat agaccgcact
     3301 tacttttgtt ggccttttat ctctaaaatt tcatatgttt catctggaaa tttcgtgacg
     3361 ttgacgttgc agatccgaca ttggtagtat tttaggggag gatcgtgact tatagagcca
     3421 ttaagccata ccatcaaagc accgcatatc ggacaattca tagtcacaat gaagatattc
     3481 accaattcct aatatgcttt tatctcctag tgctcacctt gcaatggatt agtcnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggataaac gaaatgccgt
     3781 taactctcag ccagagaaaa cccatctaga taaaaacaaa tgacttttat ttttatctat
     3841 tcgttttatg tttccttgac ttgtcatagt gttgtctact ataaaataat atttaaaatt
     3901 cgcacattta ttctaatgct ccagacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca cataccccca
     4261 ttgatgtatt ctcgcagtgg ttgactggtt gctcacgatg accatccacg ttgtgacata
     4321 tattggttgc tcattctagg cattattcca aaaattgtcc atttcacgtg acgtcaccat
     4381 ccccacttta gcaggactca caataagtga gttaatggct gtctgctaga tttctacatg
     4441 caaaagggcc ccatgtgcag cgcttgttct caactccaga cattgcagca gtactacatt
     4501 caatgttctg ttgcgaacac gggtgttgag gatggttgtt gtaagaaggg tgttatcgtt
     4561 gtgcgtcggg agctaagaat gttattagaa tacattggan nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatagctgcg
     4861 attttatttc tcataataac cccttttgta tggcgcaaat caatgggcaa gctagaaaac
     4921 gcaggttggg tggtcgcgat catagcagct ttggtctcga ttttgcctta tccactgcag
     4981 gatttacgtc ttcgttgggg cattttcatc gcagctctat tagccattct attcctagcg
     5041 gtgggacgtc gcccatttgc aattattacg aataaaattg caaaaaagcc aagtgatata
     5101 c
//
LOCUS       DAOR01000268           15426 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20136, whole genome shotgun sequence.
ACCESSION   DAOR01000268 DAOR01000000
VERSION     DAOR01000268.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15426)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15426)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15426
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20136"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    14196..14423
                     /estimated_length=228
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4131 a         3279 c         3023 g         4765 t
ORIGIN      
        1 tatatctatt gtgctacaag ctagatttgt tagtatctaa ctttagttcg ttcgtcagct
       61 ccttaacaga gcgcgcaata tctttacaac tcagagctag atctttcagg ttttgaattt
      121 ttttattgaa agttgaattc attccaacga tagttgatgt attccatgcc tcttctatga
      181 tacctgttgc gtctaattcc gatcttatat tccttatatt ctccgcaaca ttctgcattg
      241 cttctgaagt cagattcata ttttctatat tttttagcat tggcggagcc ttcaatgaat
      301 cttcgatctc ttgtatagtc gtagaaattt ctctgatttt ctcaagggat tccatcattc
      361 ccggtgagcc gagttcagta agcctcttga tcgccctggc aggggctttc gtgatatcct
      421 ttaacctcat gggttattag agcggagatc atgttaattt gtttatttgc ctatcttttg
      481 ttgtttgtat attccccgga atttaaccac cgaggcgatc atggtctacc tgtgccgagc
      541 aacattccta ttgatatctt tgttttttca tttcgttttt agctttgcaa tgaaatagac
      601 aagtcatggg aatactacaa gttggtaggt ttgattccta atcctttata tttagtattt
      661 gtcgccatat ttcgtgtttc caaagaaact cgtttaacac aaatgtaaat acggatataa
      721 atgtaaacag ggctgtcaac aactggctca tttgcccttg taatttacaa ttagattcta
      781 atctctattc cgttcttctt taaatagttt ttgactctgt ctacggtcga tttctcatag
      841 tgaaatatgg atgctgccaa tgccgcatca acatcggttt ttctaaaaac ttctagcata
      901 tgtctaggct taccacatcc gccagatgca atcacaggaa tgcgaactgc tttacaaata
      961 tattttgtca aattaatgtc atagccgttc tcagtcccat cggcatcaat actagtcagt
     1021 aggatctcac cagcccctag agattcaact tttctggccc attcgattgc atcaatacca
     1081 gtctcgacct ttccaccata aattcttacc tcaaaccaat atttttttcc tcgatgattt
     1141 tcgaatctgt tcttaccatc gtcgtcgtaa tttcttttag cgtcgatggc aactaccaca
     1201 cattgcttac cgaagattga cattaagtct gtaattaggt ctggattttt aacagcagca
     1261 gtattgatcg acaccttgtc tgcaccacta agtaatatat cacgtgcatc ttctaacctt
     1321 ctgattcctc ctcctactgt aaagggaata tcagtgaccc ttgctacatt tcgaacaacg
     1381 tttttcattg tctctctctc ctgttgtgat gcagtaatgt ctagaaaaat gagttcgtct
     1441 gccccctgta atttgtatct ctgagcaaat tcgacaggat cgcctgcatc ttttactctg
     1501 tcgaaatgta ttccctttac taccctgcca ttgtcgacat ccaaacacgg aataatccgc
     1561 ttagccagtg gcatcttatg agatcgcctt gacccgttca acactgagtt tgtcttcata
     1621 aagtgcctta ccaaggatga ctgcgctaca tcctatattc ctgactctaa tggcatcttc
     1681 gatacttgaa attccaccgc ttgcaataat gcttgcgctg gtgaaactaa cagcagaaga
     1741 tagtactgta atatcaggcc cgttcatcat cccatctcta tcaacgcttg ttaacagaaa
     1801 ctcctttatg cccatgttca taaaaaatat catagaatcg atgaccccca atcctgtggt
     1861 ctctctccaa ccatctatca tgaccatccc attattctga tcaagtgaaa ttacaatttt
     1921 gtttcggttt ttcttagcaa gctgtcttac cgaatcagga ttcttgtaag ccattgttcc
     1981 gagtacaact ctagcggcat ttttgtggaa catttccttt acggctggga ctgatcgaat
     2041 acctcctgct acttctacag ggatgtttac agcaccaatt attcttgaaa caatttcaga
     2101 attattgcgc ccggttcgta aagtcgcgtc gaggtccacg acgtgcaaca tatctgcccc
     2161 agccttttcc cattttttag ctgtctcaat aggatcgttt ccataaatca ctttgtccgc
     2221 aggattacct tttacaagtc tgacgacatt tccgtccatg atatctacgg ctggtattac
     2281 acgcatatct gagaacacgc ttttaaaaaa ttcctaagta tagacgcgcc tgtggaaccg
     2341 gatttttcag gatgaaattg ggtgccaaag agattcccat tttcgactac ggctgggatc
     2401 ttaatgccat aatgagaact cgcagcaact aggcctttgt ctttaggctt tacatagtag
     2461 gaatggacga agtataccca tgaaccatcg tttacacctt tcaagagctt gctatctttc
     2521 cgggttacgt ataaattgtt ccaacccatg tgaggaatct taactcttgt tctagcaaac
     2581 atcaccactt ctccgtctaa aattttgagg ccgtctaaaa tcccctcttc acttttatcg
     2641 aataacatct ccatgcccaa gcaaatacca agcataggca cgcctccatt attggcatca
     2701 tcaatagctt gaatcaataa ttccttatgg ggctcaatag atcttatcgc cgggtcgaaa
     2761 ttgcctaccc caggtagaac taacccacta aatttttcta ttccttcgat gtctcgtatt
     2821 atcgaaactc tctgtgcacc attccgttcc aaagcggcct ttaggctaaa aagattgcca
     2881 gctccgtaat caaatattgc gatgttctgt gtcattacat agcacctttc gtacttggta
     2941 cccctcccct tcttctctcg tcgatgctgg cggccattct aaacgctaca gcaaatgctt
     3001 taactgctga ctcaatttta tggtgatcgt tatctccgta atttaccaca atatgggtac
     3061 atgcgttcaa attctgaata agcgatctca caaaatgttc aaggtcttca caggggattc
     3121 cctcaacagt ctccgcagct aagttcaatt gcatattata aaattgacgc ttgacgaggt
     3181 ctatcgctac agatgctaat gattcatcca ttggaacgac cgcatgcccg aatcttctga
     3241 ttcgtgatct gtcagaaagt gctctgtcta tagactgaga aagtgcaatc gatgtatctt
     3301 ccacaagatg gtgtagtata ccgtcattgg aaatggctgt caaactaatg tcaatcatac
     3361 tatgcttggc aatcgctaca attagatgat cgataaaaga aagccctgtt tttactgctg
     3421 ttttacccgt gccatcaata ttcacttgta ctgcaagccg tgtctctttg gtggccctct
     3481 taatttttga aattctctgt gtggtattat gtggataagc gaccaaacgc gatcactctt
     3541 ttacccatac tttatttaat atctttggta gctgattgat atcttgaatt atgacgtctg
     3601 cgcctttttc tttgaattgt ctcatcgttt ccagaggttg cgaactgcat ccataaatac
     3661 cacaaaatgc aattttgagt tgagtttttt cctcagctct tctgaccatt atcaagtcct
     3721 cgtaagagtc cccggcataa atcgcggttt tggtgcccat gcttttcata gccttcataa
     3781 tcccataggg atttggcttg gcatactccc tcctttcgtc ttctaggaat acggaggatt
     3841 cttgcttgag agtgtctaat attggctgga gagaatattc tgcagctaat ttgcttctgc
     3901 ctgatattac agctagattg ccaccaaatc tccttgcaag gaggtccatt gttcttctag
     3961 ttatcacaag cctgtcattt tcaatcagcg gttttccaaa gtaaaatttc ggctcaaaat
     4021 tgtgcaattt tttaaataat tctggtccat agaataattc atcgaatact gttgcaatta
     4081 cactcctctt accggcagag gcaggataat ctaagagttt tttaaggttt tttataatgg
     4141 tagatgaagt taaagacatt agatattttt caacggaaag tatcccatct tcatcggaat
     4201 ttgcagctac tttatttagg aactctctgg cgttggtaag atttttttga gggttagcaa
     4261 gctctgcaag acatattgca taacttgtgt cgacgtcatt gttgaaacct cctgtttgtc
     4321 taaactttag aatgatgtta tcggatacca gattggtaag cctgaagtta tctattgctg
     4381 ttgtagtagc aaaatagttg agtataaaat caacagtttt ttttatggca gcattgtaag
     4441 atttccttat atcgactagg cacccatcga cgtcaaaaag aacacactca gagttcaata
     4501 acaataattt tctgaatttt tttataggat ttgttttttt atatgatttg cggccaacca
     4561 tctgtttttc aacctcttgt ctagattgcc ttcgatattg atttgagaaa tttatcgttc
     4621 atttcacttg ttccgattgt aacccttatg cagcctctgt gtccatcaat atctccgaac
     4681 gctttcacag ataatccttc tttttttaaa acacttaata tccttccata actatcatag
     4741 gattcaagga aaataaagtt tgcatctgac ctgaagacct ttataccgtt catttcaaag
     4801 agctcatcaa ataccctttt tcgttctctc ttgattaatt ccacagtttg gcttacatag
     4861 cgctcatagg agagggctaa ggttgctact gctaacgaaa aactgtttag aggataaggc
     4921 gactggattg ttgttctaaa tatttctgca aacttctcat tcgcaataag atatcctatt
     4981 ctggctcctg ccaaaccaaa tgctttagaa agagtacgca atatcacgac attgtcgtat
     5041 tttgcaatat gtggagctaa attataatta ccaaattcaa catacgcttc atcaattaag
     5101 atcaacttgt ctttgaatgt gtctatgatt tccaaaacca gtttctcacc aaattgatta
     5161 cctgtagggt tattaggaga acaaatgtat attatatcag agcgtgcggc tctcttcaag
     5221 aattccaact tcggtagctc ggcgtcttcg gttagtggca ctctatccaa cgcaatatca
     5281 tgcaattcac atcgatttat gaaataagag aatgttggag taaaaatgct ggcctgttga
     5341 tttcttccga agatggaaag gatcaattca attatttgat cggatccact gccgatccct
     5401 aggtattttt tatcgacacc cgaataattt gaaagccgtc tataaaggtc ttcgaattgt
     5461 tcgagtggat actcgcgcag atcagtctgc tttatagcat gtaaggctat tttcgagatg
     5521 aatttaccat caagagcgaa attctcattc gcatctaatt ttattgaatc tttgattctc
     5581 tcaggcttta cgtaagcatc aagtttgcaa attttatcta atctatctga cagccagttt
     5641 gtcatttggc catcgttctt gcctgcacgg ctaagtaatg atttagtaat ccttctgctg
     5701 aggcaatttc tctgatattg tgttgtactt tctctagtcc agacttggtt gcttcgatag
     5761 tagacaccag ttttaaaaag tcgagaaccg aaagcgatcc cctggatttc ccgaagccaa
     5821 gcgtaggtag aatatggttg gacccaaaac aataatcact agctgaagat ggagtatatt
     5881 ttccgattaa tacaagtcca gacgatgtta tcttcttaga cgtggctcga gcatttttgc
     5941 ctaccagttg cagatgttct ggggctattt catttataaa ttctactgca gtagatcgct
     6001 gtttgcagat ggcgataaac ccgttcctct ccaagctttt ccttacgata tcagcacggg
     6061 atatactatt agatttaaga atggagttta tctcggcgga gacacgtcgc gccaattgct
     6121 tagaggttgt aactaaaccg caaaaggtgt cataactgtg ctcggcctgt gaaactaaat
     6181 ctaacgcgat caattttggg tctgactttg aatctgcata aacgagtaat tccgtaggtc
     6241 ctgctaccat gtctatcgaa acctttcttg aggcgagaaa tttggcggcg gtaacaaaca
     6301 ttcccccggg accaacaatt ttactaactc tttctatagc gggtgttccg tatgcaagag
     6361 cactaatccc ttgtacgcct cctattttat aaaattcttt caccccacat atcctggcgg
     6421 ccacaagggt tagaggatct actgttccgt ctcttagcgg aggtgatact gccactattc
     6481 tctttactcc ggctactttt gcaggaacag cgcacattac catcgtgcta gcataacgcg
     6541 cattacctcc gggaatataa catccgacac tagaaatcgg ctgaattaat cggtgtacct
     6601 ttatcccagc cgataaaatt gtgattccct tgaggtgatt catgactgaa acttcgcttt
     6661 ccattaaacg tctcttgata acgttaattg cttgaatctg cttcttcgta accatagagt
     6721 acgcctgttc gaattcttcc tcagtaactt ttaatgaatt taaccgaact ccgtctaact
     6781 tttcggtata gtcaataatt gcagcgtcgc cacgctcagc cacgtcattc atgatagaag
     6841 ctactctctg aattagctga tcagaaatac tgatgcccga gttgcgcaac tttgccgcgt
     6901 cttctttggc gtttgttatt gttatcgtct ttatcaagca tgctcttcta gatttatctc
     6961 atctagcggc agaatttgac gaggctccat aacaactagg ccttgtgcaa tcttcctcat
     7021 cattggaaca agtttgataa attcattctt gtcaatgatc gtgtttacac cataccatcc
     7081 ctgctcgctt aattcgctaa ttgtcggctt tttcaaagca ggcatatttt tcaacaaatg
     7141 gtctagattt tctttcttta cattgacgaa aatgtgaagc tttttacggg cttcaacgac
     7201 acctctcagt aaggcaatca tgtcggcgat tttctctctc ttcttcgtat ttcttagggc
     7261 gcctctattg gcgacaacta ctgccgatga ttccattact atatcaatta tttttagatt
     7321 gttctgtccc aacgtggtac cggtttcagt aatatcaaat attgcgtcga catcttctgg
     7381 gggttttgct tcagtagccc caaacgaaag gaatatttgt acgttcttgt tttcccctac
     7441 ccttagccac ggggttatta tcaaaggatc tagtttgcca aagtactttt ggtaagcctg
     7501 attggattta aaatgagcag aagtagttgt gaggtattcg gaagatatcc ggagtggctt
     7561 cttttttgct gcaaattcct caatcagacc tgtcaggtct tctactgcaa acgattctgg
     7621 gacggctata acctgcttga cttttccgta ttctaaatct agtaacgttg ttatatctgc
     7681 tttcgcctct ctaacccaat ctttacctgt gattccaacg tcgtagaacc cttcctggac
     7741 ataggttggt atctcttggg ggcgtaaaac cttaacctct atctcgggat cgctcagctt
     7801 tactcgatag gtacgtcccc ggccactgat gctctgccac gcctgctcta ggatcttgaa
     7861 tgtggcttcc tcaatactac ctttgggaat tgcaaatttc acggtaggca tactggacaa
     7921 ctcaatcgca aagtctgttg atgggaggca tatatttact tattattatc gtaggtatta
     7981 ttattaagat tcttctcagt catcagggcg ataatttcct tagccctatg tacgttcaca
     8041 ttcttctcat cagcaatcct ttgggctact aacttaatta aattgcctct ggcccctgcc
     8101 aacaccgcaa tatttctagc gtgcagcctc atatgacctg cctgaattcc ttctgacact
     8161 aaggctttca ttgcagcaaa attttgagcg aggccgaccg ccgctaatac tgctgcaagc
     8221 tccttggctg atttcacacc taaaatcttt attgcaagtt tggtcatggg atgaactgat
     8281 actaccccgc caacagtacc aactgctaaa ggtaactcta gctcaccagc caaatcgccg
     8341 tcatttgtct tgtaccacct tgtcatagat ctgtacgtcc catctctggc agcatacgcg
     8401 tgagctgccg cttcaatcgc gcgaaagtcc tgtcctgttg ccaacgctac tgcatctatc
     8461 ccattcatta tgcctttgtt atgagttact gcacgatacg ggtcagaata cgcaaaagcg
     8521 tatgcataca aaatacggtc tacgatagcc tctcctccca attcatctct ggcaaatatc
     8581 gccttgcatc ttacgaggcg tctagtcgaa taatttgaaa gtatccttac aatgactttg
     8641 ccgcctgtta aaacttcgag ttttggagat attgcctcac acattgtatt tattacattg
     8701 gcgcccattg catctttagt gtcaacaagt aactctatga ttagcatctt ccccatcata
     8761 ttttgacttt tgtcttctat ctctttgatc tgaagatctc ttgctataac ggatctacta
     8821 ttcgaatttg ccaagtttag taattcgctt ttgtgatgta aaatcttctt tatagttgac
     8881 cgcgagagat gcggtggtat ggagactagt tgcacttgac ctatcatcaa tgcttcatcg
     8941 acgttggttg ccgtaaagcc ccctctttgg cttgcaattt tcgctgcatt gcttgcggca
     9001 gcaattacgg agggctcttc tataaccatt ggaatcaggt attccttcgc gtttatcaca
     9061 aattttgttg caatcccaag aggaagtgaa aatattccaa tcgtattttc aatcatctga
     9121 ttggcattgt caaaactaaa ggccgcattg gggtctgtga tggtatcaat gtccctctcc
     9181 gagagatctg cttcctttct caagtaattt agacgctcac tacgatccat ttcgcggaat
     9241 tttttcttcg agtccaaaat ctaactgctc aatgactaca tctattatag agttatctga
     9301 ttcttaaaat cttgtcatca ttcgggcctg gaataccgaa tccatcttta ttgctcgtgg
     9361 caatgagaag cgagccgtca ggtgcttcaa tgatatccct tagtctaccg tatccttcca
     9421 atatagtact ttgagtgttg gaatccaagt taatctcacg taagtatccc ctcaggctag
     9481 cgatgattag atcgttttga taccccagtc ggtttgaagc agcaaatata gcgccgctgg
     9541 gttcaagagc gggactgaaa catattactg ggtcttcgag tttaccaccg ccatgttgag
     9601 tattagctgt gttggggtta ttgtggctac taccactact agcggtacta gtattactaa
     9661 tagtgctgcc gagagcacat tcctttattg gccatccata attcccccta ggttttatta
     9721 tgttaatttc atcgttgcct gaccgacttt cgtcgagtgc atagagtcgg tttgttctcg
     9781 gatgccatgc gagtccctga acattttgaa agccgtaagc ataaactggc gaacctgaaa
     9841 gtgggttatt aagagggata gttccatcag gattgattcg taagatctta ccggcaagtg
     9901 agttgagatc ctgcgcaaga ttgggcatca ttgcgtcccc tgttgaaaca tataatttgt
     9961 tgtcaggccc aaactttatt ctcccgccgt tattgaattc cgcagctggg atagcatcaa
    10021 taatcacttt cgaatcaaca actttgtcgg caatttcttt gagcattatg actctgttga
    10081 atattttgtt cccatctgtg tatgtgtaat atgcatacat caaatgattt tcagtaaagt
    10141 tagggcttaa ggcaaagcca agtaacccgt cttctccaag ttgcacagct ccaatattta
    10201 ggattgaatg gtcagcaagt tttccattgt taacagtcat cgccataatc tgcccaattt
    10261 tttcggcaaa aaatatttgc ccgtctgttg aaacatccat ggccgatggc gattctatgt
    10321 tttgcacgat cgaactaatt ccaattccgc caccagcagc actgctttta ctgctcccat
    10381 tgtttggagc cggcccccca gcaataccaa cactactact ggtgctgata ctattatgag
    10441 gttctggtaa caggatagta gtctccgctg gagttaaaag aatggatatg gtagttgtaa
    10501 taataaccgc tattgaagct acaacggcca ggattaggtg tcgtcgattc atgatattaa
    10561 gaaagccgag cgaaggtttt ttaatgtaat cccgaggatc ttttgttata ctactatgaa
    10621 aatttctgat attatggtgg acaaagtttt cgcattgtca ttagatgaca cggcagcaaa
    10681 ggcgctaagt atgatgcatg aaaacaacat taaccaactc ccagtaattg ataacgacag
    10741 aaaatatgtg ggaatgatat ttgcaaagga atttctcaac cttaatactc cgccttcatc
    10801 aaaattaaaa aacctcgttg taaagacacc tatcctgagt tcaaccgata ctattgaaaa
    10861 atgcacccaa ttaattgtaa ctaccggtaa tcgagcgttg ccggtagttg aaaaaggaaa
    10921 attgattagc atcgttagcg aaaccgacgt agcacttact gcgaattttg gtcatgcgac
    10981 ggtggatgag gtcatgtctg gcgctatagt cattgaagag aattctacgc tggctaacgc
    11041 tttggctaag atgcgcagat ataacatctc acgtttgccg ataataaagt caaacggggt
    11101 actgacaggt attatcaatg ctttagagat tgccaagatc attgcgacac ctagagaaag
    11161 aattggaaaa tcccgtggcg tgaccgtgac gtcgactgct attcgggacg tcaaagtgaa
    11221 ggatattatg agaaaagcaa tctcagtcga gagaggaacc aaactgaaca cgctggtcga
    11281 aagtttcaaa agaaacgagg aaatcgtggt tgtcggagac gatcgaccga ttggtatagt
    11341 tacccctaga gatgctctgg aaataactgt tccccaccga aacgaaccca gtattcatat
    11401 cgcgcatgtg gatggcgaag cacggaggac aatcgaggaa cagatggcca gattcttgaa
    11461 gaagattcat gggaaacttg agaatatcca atctgtaatc atctatgcag acaagcataa
    11521 gacccgcaag tattctgtaa gagccagact aattgctgct cgacgtgtaa tcgacgcaaa
    11581 ggcggtgggt tatgaccctg tgagtgcatg caaggagtta atatcgaggc ttgacagaca
    11641 aataagatcc gaacacagtc agaaactcga acaacggcaa catagcaagg aatctgctaa
    11701 gaaagaggcg caacgcaaca ggagagtaga agaaagtggc gcagatgagg agatttgaac
    11761 tcctgatcgc ccgagggcga atcggcttgc tttcaggcta ttatccaaga gatgtatata
    11821 atagactcac tcaaggccga cgcatttttg tgttccccga gctttcttgc tacctgtgag
    11881 aagaccactc tgccacatct gcgcacgcgt gtttgattac ggcacgctac ttactattat
    11941 gtgttaacta gttcttcctc gggcgatcgc atccgtaata acaagaaatt gcgctccatt
    12001 ttttcttgaa ttatccttta ggtaacccta aatatattaa attgttcata ttgatagact
    12061 atgggtgaaa caatcgagct agacagcttt gtcgtcagtt tttcttttcg ctgtccaaag
    12121 tgcgagtatc tgaacgaaga aatttcagct aatgaacttt ctagtggcaa cgcagtatgt
    12181 ggaatgtgcg agactctctt taaaatggaa gggttagatg cagtaaaaat tatcgcttca
    12241 ctggaatagg ataataggac tgcaccacga cgtaaaatat taaaaaaggt gttgcgatag
    12301 ttttttcctt tataacccat attacctgtt aacaggcggg ctatgattac tgcatttctt
    12361 acttgtgtat tggtcatgat tggaacctaa gaatcttaaa atatcaaatt aagggtttga
    12421 aaatcaaatg gcgacattac caagtaggaa atcgatagaa gggccagcta gggctccgca
    12481 cagggcaatg tataaggcaa tgggtcttac agatgaagat ttggataggc cgatagtagg
    12541 agtatcctca acctgtaatg aggcgacccc ttgtaatatt cacctatgca agcttgcgca
    12601 aaacgccaaa caaggcgtta aagattctgg atgcacgcca cgagaattta ctgccatcgc
    12661 ggtatctgat ggaatagcca tgggccatga ggggatgaaa gcttcgctgg tgagtagaga
    12721 gattatagct gactcgattg aagtgatggt aatagcccat cagtacgatg ctgttgtagg
    12781 tctctccggc tgcgacaaga gtttgcccgg aactttgatg ggtatggctc gacttaatct
    12841 acctgctatt tttgtctacg gcggcacaat catgcctgga aattggaacg gcaagcctgt
    12901 tacaatccaa gatgtctatg aagcggttgg tacgtttgag gcaggtaaga tgaccaatac
    12961 cgagctaatc agtttggaga atgcggcctg tccgtctgcg ggctcctgtg caggaatgta
    13021 tactgctaat actatggctt caataagcga agcaatcggc atggcgttgc ctggaagcgc
    13081 ttcttcacct gcagagagtc agagaagatc ggaaatatgt tatgatacag gtaaagcaat
    13141 tttcaatttg atagaaaata atatccgtcc aaaagatata ttgacttttg aagcctttga
    13201 aaatgctatt gcggtcgcca acgcgattgg aggttcaacg aattcaatac tccatctgtt
    13261 ggcattatca agggaaatag gggttaaact caatatgaga gacttcgaaa gagtccgaaa
    13321 aagaactcct catattgcag atatgaggcc aggaggatcg tatgtgatgc ttgatcttga
    13381 taaagttggg ggtgttccta taattttgaa gaatctatta aagaaagagc taatccatgg
    13441 agaaaccatg actgtaacag gaaacactat gaggaaaaat cttgaatcaa tacgatttca
    13501 gatgccttcc tcttccacct cgccttctca gtcttcctat tcttcctcgt caaccgcccc
    13561 ctcctattat tcagatcaaa atgtcataaa acctatcgaa gatccaatac accctgtggg
    13621 aactcttgca attctcagag gttcgttggc accagatggg gctgttatca aaattgctgg
    13681 agtgggttcg actagatttg ttggaaaggc tcgtgtcttt gatgctgagg aagccgcttt
    13741 tgatgcaata tcgaagagga aaattgtcga aggagatgtg gtggtaataa ggtatgaagg
    13801 accaaaggga ggtcccggga tgagggaaat gcttgcagtc actgccgccc tcgtcggtca
    13861 gggattgggg gaaaaagtgg caatggttac cgacggaaga ttttccggtg cgacccgagg
    13921 attcatggta ggtcatgtgg cgcctgaggc catggttggc ggaccaatct ctttggtcaa
    13981 ggacggagat actatagagc ttgatatcaa gaaggggaaa gtagaactag agatatcgaa
    14041 agcagaaata cgcaagagac agaagaaatg gaagccgatc aaacctcact acgttagtgg
    14101 cgctctggcc aaatacgctt ctttggttag ctctgcatcc gaaggtgcaa taactgttcc
    14161 agcactttaa ttaatttggt ttgattcatt aaattnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnaattaac tgaatttcag cgtcaatctt gaactagata
    14461 tcttaggttg gagccataga cactgttaag ctttttgtaa agtgttgagc acgtgagagt
    14521 atcgaatgta atctgcgtat cctcaattgt ttccttaact attcgttgct gatcttgtat
    14581 ttcctttcca cacaaaatac atttgggttt ggttttatcg ttggttttgg tttttaaatc
    14641 agaatcatcc tctgtccatc cttaacctgg tggtactgct atcgccgttc gctcttgtgg
    14701 ggtcactatt gaatttatta tcaaaattat tctctccttc tcatatcttt ctcatataat
    14761 tattagccaa ccgaatagag agtaaattcc tgttaaagcg aacctgcaac aatgggcgga
    14821 tcatagataa taagtcatat caaatctaat taatagacat tttggtacta ctgtactaaa
    14881 aaaaaatact aactgcgagt atgtaacaat agttaatata aacctggaac gtttcttgaa
    14941 aatttagcat atttcctttg cagagaaaga taaacacgga cagcgaatag ggctttgccc
    15001 tcagctatgc cgattgtatc ttggaagtca gagcaaacga tatgaattaa ctcttcccct
    15061 tttttagcac gaattttttt ttggagcgag ctttttattt tcaccgttcg tccgacgaaa
    15121 tgatacactt agataaagtg gcttaatccg acgttaatga tgcagagccc ggacatacac
    15181 ctaatgatac tgaacatgct attactagtg tcctgctctt acgatttttt atcgctagat
    15241 ggttcgttct tttttctaag ataataaatc agaactatta gaataagtcc aatcaagata
    15301 tagtttgtcg gagggcggag agcatcggta attattccta actgtggtat gacgtagacc
    15361 accttaccaa tgtagttttg ttgaaagata ggataatcta gggaagggat cgaatcagcg
    15421 ttggcg
//
LOCUS       DAOR01000269            3008 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_101071, whole genome shotgun sequence.
ACCESSION   DAOR01000269 DAOR01000000
VERSION     DAOR01000269.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_101071"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          920 a          590 c          575 g          923 t
ORIGIN      
        1 ggaatgaaga ttatcaagga tcttgcataa ccttttcagt aacagacaga tgaaagatta
       61 tcggagatat cttttggaag cttagactca atatttggag accattttta ataatacata
      121 ttgtcgtcat catgcgaagt cgcaagagta tttaaatgtt tgcacaccaa agtgtttata
      181 tgggtatata ccattgtgtt aacatgatgg ttaatcaaac aagaacaaag ataataaaag
      241 tcggttcaag acataccatt tatctagaga aagcctttgt agaagacagc caatttccgt
      301 tcaaaccaag tcagccgttg tcagtcagga ttgataagag caggctcata atagagaaac
      361 aatgaatcgt aattttatca agtgttcttt caccacaaag aaggattcat atagaacatt
      421 tatctgcaac aaaatacttc attacagata ttgattagca gcaaaaccat attcgtggtc
      481 gcgataccat tagaatttaa tgatttagtg ttgatattca gccttgtcac agccgcggcg
      541 ggctattttg ttgcacaagg cttcattatc cgcagattac gaaaagaaac caaaaatcaa
      601 cgcgaagtga tgaaggtaat gataatggtt atagcagaga cgatggacaa ggacaaagca
      661 cttcaattct ttgaaaaagt gttcatgcag aaaggaagat ggggctaatg acacagaaat
      721 tatcgtcaga agaaatccgg atgaggaccg acttactcag acaatttaga gagggttcac
      781 tttctcccca gaatgcccaa atactaaaag cacttttgga aagagagaaa gaagaaatct
      841 ggaatacagg cgatcttttg ttactctttg taataatagt gctgctgggc tttgtgattg
      901 cttatttggg taataatgac gttgatctga aatctttagg gaagactatt gatgatttta
      961 tatctggtaa gaagaaaaaa tgagcaaacc gcgaaagtaa ccgcccatct aaaacccgcg
     1021 ataagtaacc agtaccctaa attgggttct gttaatatag cgggtcacgt cagaggctgt
     1081 taacagtcgg gtcacgggtt tttttcgctt ttacactttc actaagattc aggtgttcat
     1141 tacgatccac tttcacggtg tctacataga tcacaaacga gctccgttac gtctgctgta
     1201 tctggtattc ctacatcgac tattgctgaa cgctctcgca ttttccctat cttgcaatga
     1261 ggacaaatag gagcatcgtc taacataatc aaatcctctt tatgtctttg ctgagcgttt
     1321 tttcgtcaat gcttttcaat gtcgtgccta tattttctaa tgcgtctgcc attaacgtaa
     1381 tttgcttttc tatcgatgat aatttctctt cggatgtttt accattactg tttattgagg
     1441 ataacatttt ttgcgctata acacgggtct tgccttgctg ttcgctagcg aacgactcat
     1501 ggtaattagt ataattgatt tcagctgcat cacaatcaag taattcggtt accttataca
     1561 tttcagcatt tttttcagcc cttacatcgt tgttcttgac gtattgataa aacgtcgtca
     1621 cattgtcata gcttccgctt gcagtaatct ttacttcatt attatccagc atgttgactg
     1681 tatgcaattt gatgttctaa tcacccgctt taccaattaa accagcccgc aaaccaacat
     1741 cgaagatttt ttggctgtct cctgtcacac agaatcgttt ttcgaccatt attattacgc
     1801 cttaattttc atcctcgatt tcagctctag agtcccgttt ttagtactat ttgcgtctaa
     1861 ttcaatccct tccctccaac caagacgagt cacttcgtta tcaggcaaga ccactaccca
     1921 cttagagtat tccttatcgc ctactctacg attgaatctt ttctgtaatt tcactttcat
     1981 tatattattc tcttcctgtt taattaacgt taggagccta aacccttaaa tacctaggca
     2041 cctaaacatg cataaccgcg aagaacaagg acatttaata gcacaaatca acggctctgt
     2101 taaaagaatt aatgaaaaag aatatgttgt aaattctcaa tccaatggtg gcatttatca
     2161 tgttcatatg agtggcctag gctcgaattt tgacaataaa cagggagatg tccggcattg
     2221 ataaacggaa tatgtccggc caagcaaacg atgtcccttg agccctggtg gtgttaaaat
     2281 atattcacat taaattatat ttcaatcact cgctctttgg ttgtttaagt gcaaataatc
     2341 gacagcactt ttatgaaaat ccgtatactt aaaaaatggg ttgtgtgttg agcgcatata
     2401 atctacaaca ctttccggcc atcttgacta cataaaaaaa ttatttgatt tcaatattca
     2461 tgtgttgatt gcgaattatt aatatagtca gatttgctgc ctacgttatg aggaatataa
     2521 acatgagagg taacgtaatt gtgttgacag cagctgtact attagctgcc agcctaggcg
     2581 caacatccct atctgttgct tcatttgaac atgcaactgc tcagatgagt aataacagta
     2641 gcatgatgag cggtgctggc caaaaccaat ccaaaacgat gatgggtact tatggaccaa
     2701 acattacagg gtccgttcca ttagggccaa caatagccaa agcgattgca tcaaaggtaa
     2761 atgtcacttt atccaacgct tcaaccactg cagaaaaagc cgtaggagct aatgcacacg
     2821 ccacagcagt aagaattggc gtagtccatg gatttttagt atacatggca ttagtcgtgg
     2881 atagcaacaa caatttccat ggtgtgttgg tagatgctgg aaacggaaaa gtcctggcct
     2941 ctactcaaat gtctatggag gcgatgatgc aaagtggcat gggaatcaat ttgatgggtc
     3001 ctggtatg
//
LOCUS       DAOR01000270            3298 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_109835, whole genome shotgun sequence.
ACCESSION   DAOR01000270 DAOR01000000
VERSION     DAOR01000270.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_109835"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1498..1786
                     /estimated_length=289
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          978 a          538 c          584 g          909 t
ORIGIN      
        1 ttcccatgta gaactacttt aaactataca tcttatctaa tgaatacatc atcaaacggt
       61 gcttttagcg aaattgtgtc agtcatagca ccttgtcctt ggacatatct cgaaatagct
      121 cagaaattgt ctaaaattcc tataggaaat gaagcttaca ggaaatgggt tcaattctat
      181 tcttcaactg aatcacatag gcaggtggcc gaaataaaag agattctaaa taggcttggc
      241 gagaaagtag acgaagagtc taaagctaaa atgaaaaacc attttgccaa tgcctgtaaa
      301 tatgaatatc ttttctggga gatggcttat aacttgagtg attagtattg atttgcttta
      361 ttttttccta tcttttctgg ccgtaggtga aacccttagg gccattccac agcacggaca
      421 aaaggcgcca tcatgaagta agtaaacttc acatcgtcta cagtatttct tacaaccttc
      481 ataatggctc ttgccgacga taattttaga atatagaaaa gtgactaata atctattatt
      541 ctatgtaata ttagattgtg ttaacgattt tgatctaatt ctgattatcg actccaaact
      601 agtataaatc aaggttcctt gcaaaatgta tccaccaatt atcaaaatta aggcattggc
      661 tggctttgaa aaagttgtca tcaaatgaat atgggctgga gaagtgctat tacctacaac
      721 aactgttaat gccaatgcaa tacaagcata aagtgctttt gtgtaacctt tccagatcag
      781 tacagataac gtaatgaacg ataattctat aactatagct tcaccaataa atttggggct
      841 tccaaaagga atcgcgatta ctcctaatac agatatcaaa ataagggtga ttaatacgtg
      901 acgatctacc accatgaaat tagtatattg gtagctttac attaaagcgt tatcaaattg
      961 gtgcttatcc tgacaatgcc atgtcaagct ggaaacagca ggaacacacg ccttgcattt
     1021 cctcagagca gatagttgaa cgacaggcta ttgaaaggat cacttagtag aggttgtcat
     1081 cagatccgtt tgtttatcat ttttatagct aatgaagaat agtcttgttt caataatttc
     1141 gacttcgaat gtagaaaaag gattttgtta tatggaagag attgatcaat ggtcagaatg
     1201 catattgctg cgatgatgca tagtgaaatg atgatgggtc ctagcactgg aatgatgcag
     1261 cgttacaggt ctgattattc tatcttgtcc ttcttctctt cttagttgta attgcctcag
     1321 cctttcctta tctcttttgt tcgttggtaa catacgtaat gccataccac aacaaggaca
     1381 gaacacacca ttgtggtaac aataaacctc acatcttcta caatatttct tgctaagttc
     1441 atactgactc ttaccaaaaa tgattttgga atgcaacctc tcacatagat tcctgcannn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggg agctacttac
     1801 agagagtgtg ggagggaagg cagcaaatgt atgcggacag tactggctct ctccaaagat
     1861 aactttggaa taacgtaaca aaaagagtct gtcttggtcg acttattgac cgtgatatac
     1921 ctaggtattt gcaaatatcc accaaattat tgcaatataa ttatttaatt aaaattattt
     1981 tcccacagtt ctgtggagat taccaccaag cttaaataac ttatttgcct ttatctactc
     2041 taaacttgag ctgtcaagga aattttgttg aagttgcaaa agcagatgaa attcctgctg
     2101 ggaaaatgaa gcatattgaa gtgaatagca aagagataat gcttgtaaac ttagatggca
     2161 agttttatgc gttaagcgat agatgtagtc atactaatgc tcgcctgtcg ctaggcactc
     2221 taaatggaaa agttgtaaca tgtcccctgc atggtgctag atttgacatt acgacaggaa
     2281 aaaaagttaa agatccaatg gaattagacc tttccaaact accagaacct attcctgaga
     2341 gcttgcagaa gatatttgca aattcggctc gcattatatc gattataaag acatacgatc
     2401 aggatagcta tgaaactaaa gttgaaggcg ataaggttga ggtaaagata taggtaatat
     2461 cgcatctacc agaataacga gtggagcagg accttaattt acctagctct tatcaaagat
     2521 aattttggaa taaaccaaca agaagagtct gtcttggttg acttattgac cgtgaaatgc
     2581 ctagacagaa aaatgagctt gtcatttcga tttgtccatc cccatatact ttggtgatcc
     2641 tggtgaaatt gtattctggg tattaggtgt gacaacttga tttgttagca cagactttaa
     2701 caaataagga tatttgtcat taattcgtgg agagaataag tttgaaattt tcgaacatag
     2761 cacatatatg ataataagat tgaacaatgg aataacgtta aaaatatcca gtgccatgac
     2821 tagtctgctg ttcttattga tttctgtccc caacacttcc atcagcgctt cgtttgcaga
     2881 tgcgacagca tcaataatac caatagaaat caatcctgca gtcaagggtg tagctgtgag
     2941 caacatgaca ttctttctaa atcacacgga tgaccattat catttgaagg gattggtcaa
     3001 gaacatatcg cacttgagcg tgggttatat cgacgtccag ttaagttttc aggacaaaca
     3061 gagtggggta atcgagaatg agtccaaatc agattttttt gttgtcggtc cgatagaacc
     3121 tacagtaaca agaccattcg acatcactac tggcattact gcaggacaag gcaatcagtt
     3181 tcaacatcta aaggctgtgg taaagcttgg ctgatgaaga tagatcttga gtgctagctg
     3241 tggaggaaaa ccaggtgtca gtatatacac acgagtgaca ctagctattt acttagct
//
LOCUS       DAOR01000271           15456 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_15980, whole genome shotgun sequence.
ACCESSION   DAOR01000271 DAOR01000000
VERSION     DAOR01000271.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15456)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_15980"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    577..700
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6443..6540
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6982..7307
                     /estimated_length=326
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4670 a         2885 c         3009 g         4344 t
ORIGIN      
        1 gctcgtagat gtgtataaga gacagcagta catacatcaa ttcagtatcg tagccgggtg
       61 ctaaatacat ctttagcagt ggatgcagtt ttccagtgta gccaatctct tccgccattt
      121 ccctgagcgc agccttctct ggagtttctc ctttctctat tttccctgca ggaatttcta
      181 acaatgtttt cccggctgca tgccggtact gacttacgaa gagtacgtcc aaattatcta
      241 taatcggaat cagtcctacc gagggtacat gttcgacgat ttctttttta attattttat
      301 caccaagtac gaacctgtca agcctgagcg aaacgttacc agtgtatatt ttcttagtgt
      361 ctattacctt tatctttctc tcttgcatag tattagtcat tacatgcttc tgaagcttaa
      421 aggtgttcaa gagcgcttga ctaacggcaa aacaggctag gataccgcag cattcttgtc
      481 agggctagac agactggatt tattgtatcc agatggaagt tcaggttacc aaaaaagtta
      541 gttttatatg ccatcttgat tgtctgggat atgtgannnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcttgattg tctgggatat
      721 gtgaactata ttgacggtga aggtagcatt atcgtaccca aatccgtcag accgattatt
      781 gactatgaag aaactttcct cggcaccaaa aaaggagcca agaagcagtt tcgatatggt
      841 aaactacaca ttagagaata caaagactac tacatcgttc acatggataa agttgaccca
      901 aggaaggatc ccatgggaca tttgttgata gatgctcccg aatacttggc ggctacaatc
      961 gcaggcttga aagcggcaaa gcaagccggc tgtacgatgt atacagaaaa aaaaggaaac
     1021 aacgctttag tagatggtct tagagcagcc tgtctggctg gtactgtcgc tgccacgtgt
     1081 tcctacattt taaccagcgt tgtcaagcaa ataaataggg gcatatagac ttaatggaaa
     1141 atccaacaca gattcggaaa cgtgttagag acgaatcaaa gaagataact gcaaccacaa
     1201 ttaagaaaaa cccttcaaaa attcaggtgg taagagttgt tctaaagtta ttaccattta
     1261 taatcaattt cagaaaagat cgccgtgagt gggtaaaaaa agaaggaaaa aatgtcaacg
     1321 aaaaaaagta ccgaaaacat gcagagaaag cactaagaac tttcatagag ctgggccctt
     1381 cctatattaa acttggacaa tggctatcca caagagctga tattttgccc caaccttatc
     1441 ttgaagtatt ggccacattg caggatgatg tcccacctgc accgttctca aaaacgagac
     1501 cgataattga agacgagctc ggcgaaattg aaaagatatt tgggacgttc gacacaagag
     1561 cgctttttgg cgcgagtctt ggtcaggtat atctggcaac ttataacggt cagcaagtta
     1621 ttgttaaggt gagcagacca gatatcgaaa aaattattga aaaagatatt gccattctaa
     1681 agagcattct gccggttgca acgcgcttta tcgatccaaa tttgcgattt tccgctgaag
     1741 ccatgctatc gcaatttatc gaaacgattc atgaagaaat ggattacaga atagaggctg
     1801 ataacctaat tacaatcaaa cacaatttga agaccgatca tatggtaatc attcccaacg
     1861 tcattttaga gctaacaact caacatgtaa ttaccttgga atatgtccct ggcattaaga
     1921 ttactgatat cgcctcattg gatagggcag gaattgacag agaaaagcta gtaattagag
     1981 tgcatcatat atttttcaaa atgctactgc agcacagcat ctttcacgcg gatcctcatc
     2041 ctggcaatat ctcagtaaca aacgctggcg aaataattct ttatgatttt gggatggttg
     2101 gcagactaga aaatgaaact cgtcttaaac taatcagatt atatcttagc ttagtggaaa
     2161 aagatccctc gagggcagtt aatgtcttga tagagctcgg gacgttggag ccaactgtga
     2221 atcgctatgt ggtcgaaaaa gccctcgagc ttagcattca atcattgcat ggtgaagaag
     2281 tagatcgaat ggaggtaaga gcattgatgg atcttgccaa taagaccctg agcaaatttc
     2341 cgtttagatt acccaagaat cttgcactat atatgcgcat gacatctata gtggaaggca
     2401 tctatcagca tcacaacgtg aatttacgat ttgtgaaggt actttcacag cttctcgagc
     2461 aagaaggact tattagggag gcatatattg aggaggtaaa agattcgatt aaaaggtttg
     2521 cgaagggtat tgaacaatca gtggagcttg cgcccttgct aagatcgtat ctagatacac
     2581 agcagattta tggcaagaag cgcgaaacca gctataactt tttgggagcg agtgtgtttg
     2641 cgtcggctct gtttttaggg tctgctttag ttatgcctca taacaccaat attgcttacg
     2701 gtggttttat cgcgtcagct ggggcatttg gtattgcgct ggcagcgatt ctgaaaaaaa
     2761 gattctagag atcagacacg ccaagagggg ttatgacaag taagaccaat catctcataa
     2821 acccatgttc tatctgacac tacacgtgac ggctgactgc tctatttgac aggctctatc
     2881 tgtttatagc taatttgccg ccacatccat gtgaatcgat ccattcagca agtaccttat
     2941 gttaaagcag aggcgagatg aatcagacca tattaccgag aaattaatcg atcctaaata
     3001 tcattaactg catattttac tagtttaggg tagatcacca tattgacaat aagaacgaac
     3061 gaatcttttg ttcgtcggct taacattatt aaagagatca gtcattgtcc tctcctcaaa
     3121 tatcataaag tagagaaagt attatcagta ctacttaacg ttgcctaata cttatgctct
     3181 cctcgttagc gtcatagttg agaataagtg cctgtcttcc ccactcaacg atccagtcga
     3241 actcatttga accatcgcca tcgggactct gcgtcgccac atcctgatta gaaacaaatt
     3301 ccaatgcctc atctttactg atacggccct tttcgcttcg ttttacgaga tcaactaatt
     3361 tcttgaatgt gtctaaattg ataattatat ctcgtaacat ttttttttgg acttttgggc
     3421 tagctactat gaacaaatgt cctttttcag ttatagacac atcgcctgct gttacctgaa
     3481 ttaggcctag catttctcct gattcaagta ttggcagaag gtcatctaac tccaaacctt
     3541 gtttagatgc aattcctgct acgtctgatt ttccgccgag atcatctaag atttccaaga
     3601 atccgagaag aacgctgtaa tgaacctttg gtatatccaa gttattttat atactacaat
     3661 aagcttggca tggctgtgat ttaaattttt attttaaggg ctatgatctt aattttgtca
     3721 gtcggagtgt ataaatacaa agagtcgagt cgatattgtt tttatgcaat aagtgtcgtt
     3781 atttgatcag tatagtcata tattgaagta tcacgtttct cgcgaggtcg tggtatgtct
     3841 atgtgaactt ccttcaccac agatgcaggc ctgtggctca aaactactac tctgtctgag
     3901 agatagacag cctccgcgac gttatgagat accattataa ctacatctgg tgtagtgaga
     3961 cggttgctcc aaactttcag aatttcttct cttagggcct cggcagttaa ggggtctaga
     4021 gaagagaatg gttcgtccat aagaaggacc tcgggatcaa gtacaagtgc ccttgcaata
     4081 ccgactcttt gcttcatccc gccagataat tcacgtgggt atgcattctc aaagccttct
     4141 agacccactt cttggatgat attaagactt tttttccttc tctcatcctt tggtatatga
     4201 ttagattcta gaccaaattc tacgttttca actactgtaa gccaaggaaa aagtccaaat
     4261 gattggaata ccattgccat atggggatct acttttcctt tgaggggttc acccttcaat
     4321 aacacttccc ctgtggtgca tgtttccaat cccattatta tccttaacaa tgtggattta
     4381 cccccgccag agggtccaac gatagatatg tattcgtgat gatttattga caaattgata
     4441 ccattcagga ctactatttt ctttccacga tcatcaacgt attctttagt tatatttttg
     4501 agctcgagca aaggcgtgga gccgctttca aaaggttttg gtacattatc aaaattcttc
     4561 attaaaactg gagtcccgtc taaaacgtaa tcgtcacctc tttcgtcgga ataattatta
     4621 ttgttgttgc taggaggact ttcgttgtag ttatccttaa cgtgggcata tttgcgtgtt
     4681 ttcatgcaaa tgatacatta gatcggatat tacattgata tatatagaca accatttcct
     4741 ctagagaatt aataattttt gacttgaatt cacaattgct ctagaaattg aggattttta
     4801 gaaattaagt attccatgcg agctagccac tcatgctata ttttctaacc acttttctat
     4861 aaagccgtct ccacactaat cgattaattg cgacaatcac cgctatcatg actccaacaa
     4921 tcaagaatag cagcactgtg tttcctaaag tgtatgcggc tttgtctaag agagatccta
     4981 taccaagcac agagtatgtt ttgtttccat atatgaggaa ttctgcgact atcagggagt
     5041 tccatcctcc tccccaacct gtaatgctac cagtaataaa tgaggggtat aacgatggaa
     5101 acaagacccg tctgatgtac tggcccttgt cggccctgag tgcttttgcc gcttcttcta
     5161 tatctcctga aatcgaccgt actccgccaa ttaggttgaa tagaatgtac cattgcattc
     5221 ctgttagtat aagtagaatc gaaggaaatt caagtccgcc gggtatatac gcaacaagga
     5281 tcgccataaa aggaaagaat gcagtagcag gtatggctgc aaaggtctga aacagaggca
     5341 ttagccgttc aaaattggga ttgtgagcaa tcttgatggc tacaggtata gtccatgcca
     5401 aggttataag gtaggctgag gccaacctaa ggtatgacaa taatagcgct agtggaattt
     5461 gagagacaat ttttgcactc cgtggatctt tgtaaagtga gaagtacatg ttggaaagag
     5521 cagataatga cttgtaaatt actccgcctt ctagaacaat aaccaaaatc gcagatatcg
     5581 ttaaaacaga tagcgttata ctcacaaata atctgagatt tctcttattg tcgaaccaat
     5641 ggtggacggt gggaaatata cgaaattcaa aggcagatac taattttcct aaggttcgta
     5701 tcaacaggtt tgattttata tgtatcagcg gccttggatt tatgatacgt aagtgaatgg
     5761 gttggaatgc aactcttctc aaaaaaagtc gagcaatgtt gttgttacta tcctgcataa
     5821 tgtgatgtct agcaagatca ggagaggaag tagaatagtc atatctgaaa cgattagaat
     5881 atgactctag cggtctccaa atgaagaagt ctgttaacat aataattaaa atcaacaccg
     5941 caattgctac cagagcctct atgtattgcc cggaagctac agacttacta agtagactac
     6001 ccaagccggg aagcgtaaag gtattcgaac ctacagatat gatctccgct gctgtaagga
     6061 agtaccaccc ggcagcccaa gacagaatgc tattgtaaat caattttggt atacttgcag
     6121 gcaagtaaag tcttcggaat tgcaacaatg gatttgctct acatgcctgg ctcgtttcta
     6181 tcaattctgg cgggattgag agtacggatt catagactgc aaatatcatg ttccatacca
     6241 tactggtaaa aattaagaag attgctgcta attccactcc tattggactt ccactaaata
     6301 aagtgataaa aaatgcaata gcggccggaa agaagccaag tattggaact gactgtaaaa
     6361 tatctacgat gggtataata attctagatg ctaagggctt ccgcgctgct gtaattccaa
     6421 caataatagc tacgatgaat gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 caataatagc tacgatgaat gacaatgcta gcgctacgaa catcctatag atagatgaca
     6601 tagaatcggt cagcaagtct gtaaaactta tcgatcctat attccaaatc acagcagcat
     6661 tcgagcttgt gcctttactt atgatgatgg ttaaaggtac aactagaaca attacagtta
     6721 tgacaagtgc aatgaaaatg gcttttttct tgtccatctt tttaccactt acctcctgcg
     6781 gcactgcaac caccttttat atgatcaaga atgtttaatt cacaaatggt acaaggaaaa
     6841 agtagtaaat aaaaaaaaga gaggaatgtc atgataaatc tcccaactaa atgaaaaaat
     6901 caggcacata tattttatcg gtgagaaata ctgaagatct tgtaaaggcc attgaattag
     6961 gtgaccgaaa ttatgctcga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc cttcatcgtg
     7321 gtcaaggtag ctctgcttca gttacggtca cagaaaactc gagctgaatc aattgaacat
     7381 gttgtaaagc tgttcaggaa ggctgaagcc gtagagccgg atatcgtatg cctaccggaa
     7441 ctgtggtatc ccaaaatagt taacagcttc gaacaggagt tcaagcatat tattgatatg
     7501 gccaaggaac aaaatatgat tatcatatct ggggcctttc tcgagagaat caataataac
     7561 ttatacatct cggcccctgt tatttcaaga gacggaatga tactaggaag gcagttcaag
     7621 atacatccgt ttggggatca aaggaatgct gtaaaatctg gtaccatgat ggagatgttt
     7681 gatggcggca gcttcaagtt cggaataggg atatgctacg atgttgtctt tccggaagta
     7741 tccagggcac ttgtgcgaaa gggtgcagag attgtcttct ttcaatcaaa gataagcaaa
     7801 aaaggaatag agccttggca tatgtatgtg caggtgaggg cattggagaa taggattcca
     7861 gttgcagcgc caaatgtttg tgacgaaatg cacggaggaa agagcatatt agtggccttc
     7921 gattatgaca aaaaaaccga tattgctgtt ccgaagcgga tcgtcggttc catcaacgat
     7981 cagacactcg ggctagacat tgacttggac catgttagaa agatccacga ggcaagatta
     8041 aaagacctga agagcaacct ttacgattct ctctaatagg tacctgggca tcatacaacc
     8101 gaaaattatt actacgatct cataacctag tttgcaaata tttgactatt tcctcaaact
     8161 gaaaaaggca catttctaat catttggccg tggatctttt tgcggtaaga gagatctgat
     8221 cttaacttat gccaataatt atggctctga tagtctcaac ccatgccttc ttactgtaat
     8281 cttcgctcac acctaaaagc ttgttaactg ttaatccgtc ataaagggat actagaccaa
     8341 cagcgatagc attcgcatct atatttttcc taaaaaaccc tctttcgatt tggaggttta
     8401 cccatcctac tgcggtctcg aatatgttca ttctttgttt atacaaggtc tttcgtaact
     8461 ctgcattacg cgccgattcc gcaatggtct cgaagaaaat cctttcattc gcagtctttt
     8521 tcttgcgaaa gaaatcataa aagcgttcta tatctgacac aaggtcttcc ttcttagcaa
     8581 acattgtcga tatttgttgt ttcgcttctg cgaggttatt ttcgcagatg gcatagaata
     8641 agtcttcctt gctcttaaaa tgaagataga ttgtaccctt gcttagatct gccgtattag
     8701 caacatcatc catccttgta cgatcaaatc catattttga aaaagcatct atcgctgctt
     8761 taattatttt ttccctgatt tctatcttgt attgttgagt gacttttggg gacaactttt
     8821 actccttaat tgccgtaaca tagatactac caagttacta ataaagatga ctgactggtc
     8881 agttacatat attaagaact cttctttaat aaatacagat caaaacgaag ctagaggaca
     8941 cgggaataga agatcaacca aatttcgata tcctacatag aatttctgct aagagactat
     9001 caacagtcac cagccaacaa caacaaatcc caattgaatt caacaaagtc cttgtaattg
     9061 gcttaggtca gctaggatta cctgtagcaa aatatgtaag agaaataggg tttgatgctt
     9121 acggatatga cacaagtcca acagctatga atattgcgga gagagcatca attaagaaag
     9181 ctattaattt tagtgaattt gatgtttata ttctatgtgt ttcgacccat aaaccaggag
     9241 atctattttc cccacagata aatggtctat tgtctgtagt tgagaagctt tcaattgaag
     9301 caaaaaacgg ggcgcttcta tcaatagaga gtactattcc aaaggggaca tcaaagaaag
     9361 tatttgagat gctacagcac agattacatg tcgttcacgc cccacacaga tggtatgctg
     9421 cggaagagaa agagcatggg ataaatcagc cgcgtgttat tggtggagtc tgtgattgtt
     9481 gtcttatagg aggtatgcaa ttttatgggg gcgcaactta tcgatcttca tccgataata
     9541 ataataataa taataaaaaa agtctgggaa tccctatgca tccagtaaca gaaattgaaa
     9601 ttgctgaagt aaccaaggtc attgaaaatg cacacagata tttacagata gccttcgctg
     9661 aggaactcta cttatattgt caagcaaata acataaactt tccagaatta cgagatgctc
     9721 taaatactaa atggaatgtc aatattttag aaccaagaga aggcatagga ggtcactgcc
     9781 tgccaaaaga tacaaaaatg ttcttgcaat catcgaagtc tgtcagaagc aaaataataa
     9841 tagcagctac agaagtggat aaagattata gaatctacag acagacaagg gcacaaacag
     9901 atactgggca tttaatttga ttaacagaag tacacttcaa cagaataaac agaaagccca
     9961 ctcatcgtac aacctaaaat taactaatac agaaggcgta caaattattc cagatagggt
    10021 gtcaaacaaa tcaaacctaa gatatgtatc actggtgata gcgggagaat tagtgattta
    10081 gaagagggga tagataacag aatggcacta gatacgaata atggcagtaa tatcaacaac
    10141 aataattctc acccatccaa tgatggcaat catgactttg atgtctcttc tgatgcaacc
    10201 ttggcggtcg aaaacctttc taaggttttt gaatccgctg caggcagagt agttgctctt
    10261 aggaagatca atattacggt aaacaaaggt gagtttgtat caatagttgg accttcgggg
    10321 agtggcaaat ctactttatt aaacattttg ggggcactag ataaaccaag ttttggtaaa
    10381 cttttcatca gtgggataga tattttttcg ttaagtgaca aagagattgc acgtatgagg
    10441 aataatttaa ttggttttat ttttcaatct tataatttga ttaatagaac tactgttcaa
    10501 agaaatgtgg agattcctag cataattgct aggatgagca gcaaagaaag ggccaatcgt
    10561 tcctcgaggc ttttagaaat tttgggcatt gccacaaaag ctaaatttaa acccgcgagc
    10621 ttaagtggag gccaacagca aagagtggct attgcaaggt cacttatgaa taatccaacg
    10681 ataattcttg ccgatgagcc tacgggcaac ctagatacaa aaacaggaaa cgaggttttt
    10741 gacctactca gattactctc aagcaaatac aggagaacaa taataatggt tactcataac
    10801 gctgaccttg ccaaaaaaac ggacagatct atatatatca gagatgggca aatcgaaaaa
    10861 gagattcttt attaaatggt cataatcgtg gcaaaaagcc taaaccacgg gcgacagagt
    10921 cggtctgcga ttagaacaga aataggtaat cgaataaaaa ctacagcgat cacgacgaca
    10981 aaaattctat ctttcatatt tgcgggaatc ctcttttttt cttctattga aaatgcaaat
    11041 atgacaaccc acgctcaatt atctagctat attaccaaca attctacttt cccaaataat
    11101 ggatcagata ccgacaaagc tcctggattt ttggatgcat actggacgaa ttatctaagc
    11161 tctgcttcat caacatccaa taataacaat tcagtaaaga aggaggtagg tcctggagac
    11221 ggtgcctcca ctttagcagt agtattagta aatagaggac ggtcagatat cactggagtt
    11281 acgggctatc tcactcttcc ctctattgga tttaaaccaa ttcctggtaa aaataatggg
    11341 acgtctcagt ctgtggcaag tgcttattct attgttaaag caggaggtac ctttgttctt
    11401 tatttcgata tgtatgtttt aaagcaggct aaagttggtg gatactccac ttctcttagt
    11461 ttgaagtata ccaaaataaa tcaaattggt caactgatta caacaaatac tttacctttt
    11521 aggcttaccg ggaaggttat tttggatgcg gtgtctgaaa actcagagct aactccaagt
    11581 actcagaatc aattaaagat attaattcaa aacataggga gtgcaaatgc agcgggtgca
    11641 gttgtcacag ttacaggtat aactagtggg aatagcgtta cttctagtaa tactcagagt
    11701 actagtagta gctctagtaa tactaactct ggtaatacaa acacaagcac aacaaattca
    11761 ccatcgtcat cctcttcccc ttctccatcg tcctcttcta atccagcagt aaacattgga
    11821 gccacagctt ttcatattgg tactataccc gtaaacggat cagcagaaat agatcctgta
    11881 gtatatccta ccgcttcttc tggtgaaact gtacagaatt taaatctcca aatatcctac
    11941 ggcgacgcat acggtaacca gcagacatca aatgtgccag ttggattagt aatattacca
    12001 aacccacccc aatctgttgt gaatgctact gctaataacg ggaatgcact catcgtacca
    12061 gccggtaaaa tacaagctat tgattttaca gttgctaata gtgataataa aaaaccaata
    12121 acaaatgtgg ttatttctct tgtttcccaa tcgaattcaa tgaagattct tggagattcc
    12181 agatggacat tccaatctat gttacctcaa tccaagctga atttgtccac aaaagtcttt
    12241 gcatcttctg atttgattgg aaaacctgcc ttatttacat taactgttga ctatgtatca
    12301 gctggacaat caaagacaga ttctcttaat ataggaacgt atgttagcgg tgacatcaaa
    12361 atacgagtat atgatgttgc tattaacttc ataggcggca ctccgaacct tataggaaac
    12421 ctcttaaatg aagggaacac ggttggattg ttcactacgg ttgaattggt aaagtctact
    12481 tcaggtaaaa gctttatccc gataacccct ccatctcagt atcttggtga cctttcagtc
    12541 gattctcctc tgccttttag catgccacta aatatagaca ataggaccat gagcacaatt
    12601 cagccaggaa tctatcctat cacacttaaa attacgtata gtgatgattt gaaaatccca
    12661 cacgtacttc tagtaaatag ttcggtcgac tttgagccta agcatgctca atcaagtaac
    12721 ggcgccggag gtagtggcgg tggattatct ggctttggga tcttcgtcgt tgccgttatt
    12781 gccgctgtca tagtatttct ttttgtaagg agaaggagat ctaaatcaaa gcgtaccaaa
    12841 ttgcagagta gcaaagatgt agatccattt ttagatgatc tatctcgtgc tcctgcagaa
    12901 aaggatctaa gtcgggattt gaagaagaga taaaaataga gaatgaagag gaggcgacag
    12961 aaatatgctg aatattaaag aacttttcat attatctttt gaatctctat cggatagaaa
    13021 aattagatct attctgacta tattgatggt aatggtgggg agtgctcttt tgattgctgt
    13081 aggtgggatt ggtgcatctt ttactgatgc atttagtaaa cagttcagga atttagcacc
    13141 aaacatacta ttcatatcta gttcgcaaca agcacaagga ggtcccggcg gaggcgtagg
    13201 gggggcggga actccaccgc ctgcaccaaa aattacttta aatgccgcaa tagtaaatag
    13261 aatacattca cttcctttcg tgagtgatgt gatatctacc tatagagctt cggtaacagt
    13321 acaatctcaa agcgaatcca aaactgattc aatcttgtcc atggatcctc aaaaacttat
    13381 agtcatcgcg ccaactttag aatatacaga tggttctaca ttacagccaa ataacccagc
    13441 ttcaatgatt gtggctcaaa atgtcgctaa tccaccaggg gatgcgaatc catttttggt
    13501 attaggtcaa agtgccaagg tgacttattc ttttgtagat tctactacag gccaacaaaa
    13561 ggaggaatca aaaaatttta tagtcacagg cattataaaa gaaactggta atccaacaat
    13621 agacagcgcc attgtaatca acttgcaagc cggaaattca cttctacaaa agtctggcaa
    13681 atttgactct ctcttcgcga ttgctcagac aactaacttt gtagatgttg tggagcagga
    13741 gataagaagc ctgtatggta ataatatagg aatcactacc gtgaaggcta ttttgaaaac
    13801 catagaacaa ttcaccggtc aaatcaaagc atttcttaat agcattgcta ttatttcgtt
    13861 ggtcgtaggt gcagttggga ttattacaac gctttatact gctgttattg agagaacgcg
    13921 cgagattggc actttgaagg cgattggcgc acagaacatt gatatcttgg ccctcttcct
    13981 ggttgaggca ttattgattg gtatttttgg ggcaacaata ggattgttag ctggaattgg
    14041 attcgggtac ggtttaagcg ctaccgtagt tcccggtaat ggatcttcta gtcctcctat
    14101 ctatactgca aatgacatgg gtagggtgtg gatgatttct gtaggcctta gtatagttgc
    14161 aggtgtagtt ccagcgttac aggcatcacg attattgcct gtgcaagctt taagatcaca
    14221 ataacgagag aatagataat ccaaataaac ctaggttgtg aatgagcaaa ctcggaggag
    14281 agtctgcata agtagagtgg gttttaataa aattaacatc aagttgttga gattgaatac
    14341 ctttcaatca cgccaaaccc caactactac gacttgcaat aatgcgaaat aacaacgaac
    14401 ccggtcaaat attgcatcta ttctaccttc atgtcttgta acagggtttg taattgttat
    14461 ttcactaact atacgatttg gtgctggatc ttggctcatt ttatcatgaa gtacgcttgt
    14521 aagcgcacat gatcttgtga aatatatcgc tcaatgttac tctccggatt cccttgatct
    14581 ttttcacgaa catcagcaaa gactggttgc atatacggac tccatgcctc cataatttct
    14641 tgactattgg gggtgggtga agttcgccat agtcgagcta acgtctcggt tgcactttcg
    14701 agtacgttat gagcaatgaa gccagggatg aatgattcat tgccttctgg aatggccccc
    14761 atgactcttg gtagtttgaa tttcaaacca catgtaacag catgtcctat atcagaagac
    14821 agagccagat gatatgattc gtttcgtggt gctgcctgtt gcataatttc ttttgcgctc
    14881 acttttgttg tcttattata taccaatgac aaagtatata atacatatca atttacttat
    14941 tttactatag atactttaat gagtaattct ttaatgggtt cgcttcagat gcttagagtt
    15001 cacgttgctc ccgttggctt tgaagtggac cggatagtca tccccgcagt tcaaatgaaa
    15061 gcagatagag tctggcttat cacacacagt aatcctagtg ttgacaaagg acatcaattt
    15121 gtcacattaa tacgagataa gttaaagcag gcaagaatag aatgtctaca gacggaagct
    15181 gatagagttg aactttttga tacactaaga gcgttgagaa taataatttt aaaggaaaaa
    15241 ggcaactcca ttcttgtaaa cgtctcagta ggaagtaaga ttcaggctat cgcttcgatg
    15301 atggcatgta tgatgttcaa ggatatagcc atgataaagc cttactacgt agttccagaa
    15361 aagtatacta cgaaggatga gaaacaagaa actgaaggtg ttaaagacgt tattcctatg
    15421 ccagaataca aaatcgagct tcctgatgaa aaattg
//
LOCUS       DAOR01000272            5378 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_98579, whole genome shotgun sequence.
ACCESSION   DAOR01000272 DAOR01000000
VERSION     DAOR01000272.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_98579"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1756 a          980 c         1035 g         1607 t
ORIGIN      
        1 aggaagattc cttaaaatct agcacagaga tcctcagata atctcttgcc gcttccttac
       61 acatttggat tactcccatt tttttcttga cgtataaaag ctccgctagt cgcaaatacc
      121 agaatacctc ttttgctgcg gactttaaca agttcatgta tagctccctt gttctagatg
      181 aactttgaat aacttcagta tctgctagct cggcaccaac ttttacaact ttggaatcag
      241 gcagcatgcg ttgaacagtc tcaccggcag agtctgtatg acctactgct aaccttggtg
      301 gtagaacttt gtatgccttt ttaagaattt tttagattca tcggatgaca tatcaagctg
      361 aatgccatct gttaacgttt tagccactct agcacgaatt tggtctaata cagatacatt
      421 agtgcctcat agagaaatta gggcttttca agctaagact aaacctaaaa catacggacg
      481 gtctaactaa tttatgaagt aacaaatgag gagggagtgc tttctgggtc gtcaaatacg
      541 cccctttgaa attcgtgttc atcacctcgt caaagtcttc ctctcggttg tcgtaggaat
      601 tgtaacgata gatgatttag tctctttatt ccgaattaca tatacacgga ccaaaaaata
      661 ttctattgaa actgttgaaa tacttgaatc ggctatggat ggagacgttt acttattctt
      721 cttttcataa ataatccatg ccaagaacaa gtcgtgaaaa tagttatgtg tctccttcac
      781 gactgagtca ttgtttatgc ctttaatcct cgaacaacag aaaatgatta atgaattaat
      841 tacgaaattg cagcatacaa accacgttaa caaagaagac gagcaataaa gcaaacgagg
      901 atagcaatcc tcttaacaat attgtgacat cttccatttc cgcgagagat gtcattttta
      961 ctgctaaaag taattaatgt cgggaacgtt aaaatatcaa gatgtactga tttctagtat
     1021 atcaagatca gctgtttatc aaaattggtg ttacgttcgt cgaatgtgct tatagtaaat
     1081 atgaggatat ttggagcgtc ccattaacaa tatctgcagg gaaaaagaaa gagacaagga
     1141 gatgccaaaa caatcgggga aaatcttaga gaagatgatc ttaaaaataa tggaaaagct
     1201 ccagaaaaga gtaaaagctg caaaccagac ctgtttcttc tacgactata ctaatgtatc
     1261 gctaatgact gattttgttc caagaacatc agggaattgg gaggaggagc tgcagctgca
     1321 tactttggta taaagatatg ccagtagctt atttactaat cacttgtaac gcagggcttg
     1381 atgaagaggt gttgagcaaa ctgaacgaat taccagaggt agtcgaagcg aatagactat
     1441 cttttggccc ttacgatata attgtaaagg ttgaggcaga tacaatcgaa cggtttaatc
     1501 aaactgttgg atttgatata aaaagaatac gtaatatcac atctgcagtg acattaatta
     1561 aaaatataga agaaccagca tagtactggt agccctttca ttttttattg acttttcatt
     1621 atatgtcgtg cgaactattc agatacgata tcatattact gaaagctaag ccatgaccgc
     1681 gattttgtga tttcttcttt ggtttgcgaa taaacaaact cggcagcatc atataggaac
     1741 atatacaaat taaatatgac cgcatttact tacacaacca ttgatccttc aaaacataaa
     1801 caaatgaaag ttcgttgtga ttgggctgct aacggcagtc caattgatat tgaataccat
     1861 gacaatgaat ggggcgttcc agttcatgat gataagaagc tgtttgaatt cttattactt
     1921 gaatcagcgc aggcaggtct aagttggacg acaatactca agaaaagaga aggatataaa
     1981 aaagcatttg acaattttga tgcagacgaa gttgccaatt acgatccaag aaggcttcga
     2041 caactactta ccaatccatc tataataaga aatagactga aaattcagtc tgctgtaaaa
     2101 aatgctagag ctttcctaaa ggtgcagaag gaatttggta ggtttgactc atatctttgg
     2161 aatttcgttg gtggaagacc aaaacataat gagtggaaaa gctggataga ggttcctcct
     2221 agaacagaaa tatctgatga tttaagcaag gatctgaaga aaagaggctt tacatttgtg
     2281 ggatctacta tttgctactc tctgatgcag gcagtgggaa tggtaaatga tcatactacc
     2341 tgttgctttc ggtataagga actcgtgtag aatgtctact tgaaactagg ttatattttg
     2401 gaaacaatgc aaaatgttgc gttcagatct attttacttc ttaatacgaa tacgcaaaca
     2461 atccgtgatt cttccaacac cgacttgcgc ttatcgcatc gatctgctgc ccacaagttc
     2521 taacaatttc acattcttct tgtgaaggat atgggtgtat ctatgtatct cttatatttt
     2581 atttccaaac ttaattaatc taataggtta gtttagtcaa tgtccttatg cggaagtatt
     2641 caagtatttt caagactgtg aaatgatatt gctggttaga tattgttcgc aattcctgga
     2701 atggtctcaa gagtgagatg gaataatgag tgaggtctac tagtgtatca aaatatcgca
     2761 aacgaagacc tacttagtag cagattccca gatatgcaat gcattttaat taaagaatac
     2821 agtatgttta tatagaaacc accactaatg aaacaaaatg gatatggggt gtatggaagc
     2881 atatttatca accgtcaatc gaatgagcat tgcgaccgcc aatacataca ggctccggtt
     2941 aaatgcattg aacgactttg ttttaaaaga atataaacag aatatggata gtattgtcgg
     3001 taaaattaaa aaccataaaa tagaaccata tatgtttttg aataaatatg tggattattt
     3061 gaaatattat catagtcttt cgcccaagac gttaagccaa caagtcataa cagcaaagaa
     3121 catgttagaa tggcacgacg tcgatatcag cccgatcaaa ttcaaattaa aagtgaaatt
     3181 acccagagtg attaaaagga ataaagaagc cctgtctaaa gacgacatcg tcaacattct
     3241 aaatgcttgc tctaaaattc gactaaagac atatgttttg ttactcgctg ctacaggaat
     3301 gcgggccgtg gaagcccttt ccgtccgact cagggatttt gatactaaat cgcgcaaaat
     3361 actacttatg ggtgaatcca caaagacaag agtagaaaga acggtcttct tgacagaaga
     3421 agtcaccaag caaatagagt catggttaca gtacaaatat cgcacgcgac gtgtatgtca
     3481 ttatgataaa accaaacaaa aaacaatttg tgaaatgaga acccccagta aaaatgataa
     3541 tgatctcatt tttgccgttc accagacaaa acccgatccc aagatcatat acgtccattt
     3601 gctcactgat tttgataata cattagacag aattggcatg ggtgtaaggg aagaatatgt
     3661 aaccaatcca aagagtattc atagaaatat gggccgccgt cgaatcactt tacattcctt
     3721 tagacgcttc gtaaagagca caatcagtga tcttggatac agcgacttta gcgaatattt
     3781 tattggacat tctggctcaa catattacac caagactgac aaacaaatag tagatatgtt
     3841 caagaaaatc gagtcccatc tgacttttct cgactaccct agtctggaaa gaaaaggcgc
     3901 ggatgttgaa gcaaaggttg aaaatctgga acaggaaaat caaagactaa gacatagcga
     3961 ccaattaaag gaagacgcat tagcaacatt atcagatcaa gtaatgaagc ttatggtgga
     4021 agtacaggaa ttaaagcagc gataaatctc ctgtctacag tgtagggact aagactgaaa
     4081 tattcttgca gacacgacat tcggacttaa ttggttcatc tgataagaaa ttgattcttt
     4141 caagatattg ccttactgta gtcgtcatag gataaccaag attacaattc ttgcaatatc
     4201 cttcaagagc aagaagctcc ggatatgctc ttaaattaaa gcgggcctct tcatatgttt
     4261 taggggattc tgcgctgtct gccattcttt gctcgatatc cactttcatt ttgtataaca
     4321 gcaattgttg taggtgaagc tccatctcat tgaacgtctc atcccataag tctagataga
     4381 tattggcctt ctccttatct tttgtgtgga atataaaaag agccttactt tggagctcct
     4441 gcatacttgt aatttccgaa tgtaagtcaa tcgactctcg tagcgggtca atgataaagt
     4501 gttcaaacac tcccttgttc ctgtatcttt cgtaaagctt tgaatgaaaa atttcatatg
     4561 aagcttgggg ttgtgttttc aaaacggact gaatggcatt gtatatctct tcgtttgctc
     4621 tttctctctt gttcgggatc aggctttctg caatccacgc cagcaggcgg ccatctcgtg
     4681 tatatctata aacaggtaag tcaaatcttc ctttttccgc acgagtagta ccgccttctt
     4741 ccagtaagcc tagcttgatc agatgtatta ttttgtcttt gatgttttca gctatgaaat
     4801 caaccctgct atcgtttttt gtcttccagg tactgggatc tttgtagtga tttatccaat
     4861 gatgattatg atttagcaac caattagcca attccctatc tttgaatgaa ccatcatttt
     4921 cttgttggtg atgatcgaga tgtacgtaat atttgagaat gtcttgatac agtagtgcga
     4981 gtctagtata cgcaccaccc tccactttct cgaagattga atatatggtt ggagtcttca
     5041 atccatgaag catggtaaat ttattgatat aaaaaccatg catggcaaaa tgaaaaactc
     5101 acacttcaat aatcatacag tatctaatat ctaatatgtc aagtaattcc gacattatag
     5161 aaatcagaaa agtccaggct cttactggag aacgcagctt tacattggtc tttccgaagc
     5221 actttgcggt cgaattaggc ataggaaggg gtgatttttg caaatgtcaa gtagatggca
     5281 atcgactaat cgtggagaaa atgaatcctt aaaggagatg atgaaaacca aatgcatttt
     5341 tgtatagaaa aatcgcaata ctctgagtca atggttgc
//
LOCUS       DAOR01000273            4285 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_63203, whole genome shotgun sequence.
ACCESSION   DAOR01000273 DAOR01000000
VERSION     DAOR01000273.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_63203"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1403 a          738 c          782 g         1362 t
ORIGIN      
        1 ctcagttatg caacatagca caattgtact ataaaacaca aacagctatc gagggcaaca
       61 tgtatcactt ttcttagaga tgtaaattcg aggttctcgt tcacatataa tcccttttta
      121 cgcgactaaa tctgatttca ggaatcaatt gttgtttggg aggattttgt actgcgattt
      181 agctgattaa ttttcccttg ttgtgtagag attcgttact aattgaaaag cacatgttta
      241 ttcaaatcta taatgtttca tcttagatta tcggtttgta gaagtgttta tcaaaatccc
      301 gcctttttca tcttcgaacc acaactggga caggcaattt cctattgttg gttcgagccg
      361 aatattcctg ttgctccagg actactcatt cttatcatta tcctcctcat atacatattt
      421 agaaaatgaa tactccaaat attgcagcta gagagattgg gaatggcaat agtagagaaa
      481 tggccaagct tataccaaac gatattccga tccataggag ttggttaagg atgaactgct
      541 tgttattatt gatactcaat tttctttgcc agtctatagt tcgtatatat tgtaattaaa
      601 tctagtggtt atttcttact tacttggtag cactacggtt actgtctagt accatcgacg
      661 ttgctagacg aaattagttc gcaagtgata tatctattga tattctaaat attatagagt
      721 taaggctcac cgttcctcgg accattattg ttctaacaac ataagtatta ttctttctat
      781 gttctatacc aaccttgcct ttatgaggac ataatcgata attccttctt gatacgctac
      841 tttcaatttc aatgctgatt tataaacaat tttttcaaat aagtcataag agaaattaag
      901 aaaataagat gataatagtg atgagctccc gtctttcaac attatgtttt ttatcaccct
      961 tctattttgt atatagtaat ttgccaaggg ttcgtaaaca tgagacccta tccagtgaat
     1021 atcaataatt tcaaatccta ctttattgat catcgattct atatttttca attcataatg
     1081 ttctgatgcc cacattaatg aaaggattcc aaggttcatg acctcctgta tagttggtgt
     1141 caatagtgaa tagtatttag atagtttcat aacaggtatg gcaataacta gtaggccacc
     1201 atattccaaa attctccttg attcttggat aaattgcacc aatggtttaa aatgttgtgc
     1261 agattctaaa gcaattattc tatccatgca tttatctcta aatggtaaga ttttggctgt
     1321 agcattcact agcgatataa tctcgatagt attagtaatg gcattgtcaa gagatgatga
     1381 tagtaatgat tgttgagata ttttagaatt tacaatatta ttattacttc cctgcctcat
     1441 caactctgaa gcaactttct gttgcagaaa attgatattt acacagatta tatttaacga
     1501 agtatgaact gatttccaaa gtattgctgg ttctgagaat ccgcttccta tgtctaccaa
     1561 attcttcgca aagtccaatt cagcaacctt tccaactaat ctgcataatt cattttgtgc
     1621 ttccatggga ttttgggtgt tatcggtcca gtagccaaag tttaacatat tattaccagt
     1681 tgcacgcaga aaatatggtg ctaaaaaatt ataataattg attatatccc aatcatttcg
     1741 ccgcaaaaac cagagtgtag tttgaaggag tctttttagt tctatcgaca atgtatattc
     1801 acagttctaa ttggttaaat atatacgata taagacctat aaagtcgaat ctcgctatag
     1861 ctgctaacaa atatttgata aataagtact tttacataca ttgggggtgg gttgcgtctt
     1921 attactgtac cctattttgg gtgcttttca ttcctaaatc cttcttctcc ttctcctttt
     1981 ccgaacgatc tcagaagaaa tccactgaat atcatgactc cgacaagagc aattgccata
     2041 atcgtttttc ctataggtac attgagtgat aatgcagtaa ctaaggaaat tgccgctcct
     2101 actatgctta agggtaacca ttttttaata acaacagatg atctaaccaa attccgaacc
     2161 taaattatga taattaatgg ttgatattaa tggtttatag ttacctataa aagattattt
     2221 aaaaatccta ggctagcaga taagagtctc gaagcctggt ttatggcaga ttcaattaaa
     2281 acgtgtaaaa gtaaaccaaa gaagccgctt cagagcagat cctcttatat cgttattatt
     2341 caggaggacc tttatttaac cctcaagtgc aaatcttgaa tctaagtatg ggtggttcgt
     2401 agacagaagg aaggataagc aagtgacaga aataaaaaga atagatcgtc tgaatattca
     2461 atcaatagtt cttgccaaat ttatatgcgg gcgaattgcc gcaaattctg ctgcagtgag
     2521 aggaacgaga tgtggaggat taagtggttt ctgactaaag tcaaatgcat ttaggagatt
     2581 atttgcatga aggtccctat cagttaatgg cgataagcta aagcgccatt ctatgaattt
     2641 tagtgtagac tcaaattgat actgcgtatg atcaatgtat ccttgttttg cgtatggtga
     2701 gagtataaga gcaggcattc taaagcctaa accatactta tctatttggg gtggcggaac
     2761 atgatcatag aatccaccat aatcatccca tgtcaggatt atcgcagtgt tattccaata
     2821 cggactgctc attatagaac tgataacaag ctttacccag ttcattccag ttgtaatatt
     2881 cgctggagaa tgttcactaa tagggaagct tggaattacc catgatactt gagggaatgt
     2941 accacttttc aaatcattaa atatttgtgc tctaccgaca aagtgttggg aatattgtgg
     3001 agtgtaagta gtaccttttg cggagaaagg attccagtat tcaaatgcaa gaccactaat
     3061 tacagctccc ttgtatcctc ctacttgaac taaatgatca taatacttcc aagtaatata
     3121 tatagtggtt tatcccatct tccaacattg cccattaaga ccacagtaaa actgtctggt
     3181 acggcatacg agaactttat caattctctg cgtagtgaga aaacgaagaa tgaatacctt
     3241 cggctacttt tatactttct tacgtggaaa gggggtacta aactagatga tcttctagtt
     3301 ggcgatccta ctaaactgaa gggagatata accaaatacc taacttattt aaaacaggaa
     3361 agaaacttat ccagttccag tcgggctata gcatgtgctg ccttgaagca cttctacagc
     3421 caaaatgata tagtcctaaa ctgggatatc attgtaaagt tcattggtca agatgatgta
     3481 aaaaatcaag atagggatta tacacatgat gaaatagcaa caattctgtc aaatagtgac
     3541 atgaagtata aggctatgat tttactaatg gcctcttcag gagtaagagt tggtgcggtt
     3601 aacggtatgg tatatggaga cttggagaag gtgaaaagtt atgatatatt caaaatatca
     3661 gtatatccca aaaccagaga tcaatattat actttttgca cgccagaatc ttacaaagca
     3721 ttaatggcat atctggattt cagacagaga tcaggagaaa tactaagtcc taaatcgcca
     3781 gtctttcgct tggattttga ccccaggaat aagcaacaag taaagaacgc caaacctatg
     3841 agctatgata ctgtaaaatc aaagattagg caacttctgc tgaggagcgg cataacagaa
     3901 tacaagccaa aacgtgagaa aaatgaaaga aggaatattg tagctgcatc acatggtttt
     3961 aggaaatttg caattaccca gatgggtaga agtcgcatgg atgctgaggt acgtgaaatg
     4021 ttagtgggac ataaactggg tattcgaagt ctatatttaa aatactcaga agaagacaaa
     4081 ttaaatgagt atcttcgtgc tgtggataat ctcacaatag atgcatctaa tagattgtta
     4141 gcaaaagtgg atgaatttaa aaaacaagag caagaaatcc atgccttgaa aagagagaaa
     4201 gataaagaga tccaggaatt aaaggaacaa atgcaaatga tatttgagaa gcagaataga
     4261 gactgtctct tatacacatc tccga
//
LOCUS       DAOR01000274            4848 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_57959, whole genome shotgun sequence.
ACCESSION   DAOR01000274 DAOR01000000
VERSION     DAOR01000274.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_57959"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    4269..4561
                     /estimated_length=293
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1416 a          876 c          989 g         1274 t
ORIGIN      
        1 gcataacgtg ggcgtaggat agcacttata ataaagtaac atgtgaaaat accaaaatgg
       61 agattagaag caaaaatatg aatcgccaag cgaaaaaggt tctactgttg tcggccgtaa
      121 taatgggaga actcctatta tcaacagtaa ttccatttgc ctggggccag actactaata
      181 gcaatattgg cccacaaagc tatccgttag actccaaacc atatggtctc acgtacggcg
      241 aatggactgc caaatggtgg caatggacta tttcaatccc aaaggacatt aacccagggg
      301 gtgacacaac tggcaaagat tgtgctctaa aacagagcgg cccagtctgg ttcttagctg
      361 gaacatttgg cggagcggcc actcggacat gcagtatacc tgcaggaaaa gcgattatgc
      421 ttcctctgat caatgcagag tgtgattatc tagcaaagcc aaatctcaag gctgaacagc
      481 aactgcttgc ttgcgcaaaa tccgaaaacg aagggataac aggtttggat gttactgtag
      541 atggcgcaaa gacacaaagt ttgcataatt atagagttca atctccgtta ttcaatttta
      601 catatccaac caataatatc aatggagctc ctgtagggtc tacgcaaggc gtatcagatg
      661 gttactgggt tctgttgaag ccgttgcctg taggatctca tacggttcac attgctgggt
      721 cggtcgtaaa ctatgcaggt ggtgctctca ataattttgg aaacgaagta gcatatcaca
      781 taaatgtgcg gtaaatctaa aacccggcga tagtggcgaa ccttaaaggt cataaaaatg
      841 aaagtcttca tgtatatgta ccacaaagct caaagaatgt tggcctgtgc aatcggagga
      901 atttatactg ggtaataggg agaatatttc agtattttac gatccaagcc cgtataaacc
      961 cctgggtttt acgtattcat actggagtaa aaaatggtac gagtgggtgc aatctatacg
     1021 aagagaagag aatccagcta cagataacag cggtaaaaat tgctgtcaaa gacagagtaa
     1081 tccagtatgg tttctagcag gaacgcccgg tggtacagct aggcggaatt gtagcatacc
     1141 tgcgggtgag aatctcttct tcccaatcat caatgagatg tctactacaa ctcagtgcaa
     1201 caagagtggg cagaatttga tcaactattg tagtagcata atagatcaag ttactaacaa
     1261 agaagtgatg tttgatcatg agaaattggt gtgttcgcaa ttagatccct atcgaattcg
     1321 gactgacctg ttcgatttca ctcttccgca ggacaatctc tgtggagtgg cagcagggcc
     1381 aacagcggtt gtgtgtgacg gatattggat catgataaaa aacgaagctc tatctgctgg
     1441 aaatcacata cttcattttc ttggagagca ggcagatggt tttagaacag aagtgactta
     1501 taatctgatg atagagtaaa atagcaagtg tgtgctgtat atatttctgt gcagcctcac
     1561 ctagtagagt gggactagac gtaaagctag agtggaacga cgaactcctt ataagttcgc
     1621 tatagatata tgcatcaatg aaaaaattcc caaataaact gattcattgg atatatgaga
     1681 gggcatatta acatgacaaa tagcatagag gtctttactt taggtaaaaa gatcaacggg
     1741 aaaacttatg gcctctggac agccgagtgg tggaactaca tgatttcatc aaatgctaaa
     1801 ggcagcggag gagtatttaa attcccaaat gatgatgtct ggtttctaac atccgagttt
     1861 gtcaacgagc ctgctacccc acagattaat gccaaaatag atattggtgg gcttgtttta
     1921 tgtccatgtg acaaccagtt tattttctct tcgaaggata agtatgaaga actcgaaaat
     1981 gtttctaatc tttttgtcaa ttctgcagag aaaatcctag caacaattga aaagaagggt
     2041 ggaaatatca cacggctgga tcctgttcga gtctatacct caataagagg aatcaagtat
     2101 acgaaggatc tatctaggaa aactaagagt ggtgagaatc tcttttattc cattgatgaa
     2161 agtgatgtgg atgttaaaga gggccaggag attcaagcat ttggggatgg ccattgggtt
     2221 ttctttaaga taagtgagcg aggtgtgtat aatctgcaga catacgctca aatgcgtgat
     2281 ttttcgaata atgaggacgt ttttacgcca caggcagatt acacattgga tgtcagctga
     2341 cagcgtatgg gccgttagga tgttagtaat gactgaaagt aactatgaac tttttcaaaa
     2401 gagatgaaaa gccgttcggt aagacgtggg aggaatggac cgcagattgg tggcggtggg
     2461 ccttatcgat cccgaaagat agaaaccctg gttatgatga gaccgggcaa aagtccaatg
     2521 ctaaccaagt cgacccaaat gtatggtttc tcgtaggaac atatggaggc tcagcagaac
     2581 gtagcttcag tattccagct ggaaaagcac tcctctttcc aataattaac tttacgacct
     2641 cctatgcaga ggagcctgca ctcaagaccg agtcagaatt aatcctacgt gcgaaaacag
     2701 atattgatga cataacccat aaagaggcat caattgatgg attaaaatta gtacaagtag
     2761 atagatatcg cgtttcatcg cagcccttcg acatgacgtt gcctgaaaac aacgtttttg
     2821 gagcaaaagc cggtccgacc cgtgctgtat ctgatgggta ctggattttc cttaaacctt
     2881 tgtctatggg tgagcatacg atccatgctg ccggctcttg ctcgtctggg aaaactaaag
     2941 taaacgcgat atggcacatt acgacacaat aagttatcta tcttcagata ttggagtcgt
     3001 catcattcat acaattcgca tctaacatat tgcaatacaa ttaccttcct tgcctctcca
     3061 gtatcaccga atagatcttg cttctctaga gcaaaaattc acctactgag gttaaacttt
     3121 actttctaat tgtggcctag gtaatttgca tttcgtaggt gaaccgtcgc ttttccagtc
     3181 acaagacttc ccagtatcgc aattatcttt ttcattgtac atgtacaact gcttcccagt
     3241 tattatattt tatcaacgct tcgcagtgac tctacctttt tgccttagta gtgaattctg
     3301 ataacagtac ataaatgcca gcgagaaacg gcgcgataga ttaggcgtat aacgtagagg
     3361 tcagacacta gttactcatt ttgattcttg aattgtgaac atcctagctg aaagtccaat
     3421 gataacttcg tataagcaga attatggact gagacaaatg atgaacgtcc aggaaagtcc
     3481 taaacttttc tctaggctat catttgctta atctattccg gcaagaccct tacaaataag
     3541 gtagaccacg gcgaaaaggg gtaattttaa gttgttttct cttatcttaa aagattggcc
     3601 tttaagtttg aaatttaact gggacgatac tttcgtaata acattcacgt cactatctcc
     3661 taaaagacag gaatcattca gcggatcaaa tgattcgaaa tcattggatc tcatcttgac
     3721 taatccactc ttactattga tcgtgcctgg cattcggaat atcctatgca catccatcgt
     3781 tacttggggg tctattctaa ctcctaaggt tttagccatc ttaccaagtt cggacttgaa
     3841 accttcatat ccaccattac gtaagacgat attgcttagt ttaactacag acgattgatc
     3901 aatcactaac ttgtcagcta ttagtttccg ctaacgacgg tcaaaaaaca attggtgtta
     3961 ttacagcatt attgattgca ggcggactgt tgcattctaa aggggttccg ttctgtgccg
     4021 aagcatgcgt caaatcgtga gccagccgct gaattttaca aatcctattg agatgccgta
     4081 atgaatgtca taacaactat acaataagtg ctcatagcct agaagctaac aagaaaaggt
     4141 cagaactaaa catgaatagg actcatcttc agaacttaag ccttgaatca gagtgcgtgt
     4201 aatttctata gatttaccta gtttattctt aaaaaaatcc caaaataact acagtcctgt
     4261 agtggttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 ngtgaggcta ttcttgaata ttgtctgagg gcgggcctgt gtcaaatatc ctaagaaata
     4621 ttttctagat tatttttgat tgttcgttgc accggaatta ctcatgaaaa tcttttaacc
     4681 cgctaccccc ccaaattatg ctaagaatga atgaaacgtg gaattgtaga tgctgtgcaa
     4741 ttgcgtagaa ccagtaagat agaggaggta ttagtatatg atagacacaa caggctacca
     4801 atatacatta ttgcaggaag ggattgactc ttcatgggag tggaagaa
//
LOCUS       DAOR01000275           11067 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_16206, whole genome shotgun sequence.
ACCESSION   DAOR01000275 DAOR01000000
VERSION     DAOR01000275.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_16206"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3253 a         2348 c         2417 g         3049 t
ORIGIN      
        1 ggtatttgtt atgtacaatc ctttcacaga aaagagacca caaaggcgcg cgccgattat
       61 tccataacct tgcagaagtc acaggccata acaaagtcaa tcaaacaatc aaaagcataa
      121 catctgatgc gctgccagct tacaaaagcg catacaaagc tgaatttcta aatgacgtag
      181 caaccaaaac agaaaatcca ccgaaacata attttgcatc tggaatcaga ggcttagtcg
      241 acaataatat catggagcgc gtcaacaata ctttgagtgg acgcgagaaa aattacagag
      301 gattaaacgt tgatgatact ccaatgattc cactttttgc agcgtattac aatttggtaa
      361 gagaacacca agcaatcggt aagacgcccg caaaagctgc aggtatcgat ttgaaattag
      421 gacacgacaa atggaatgga ttgataaaac gcgcccacat atacaataaa acaggcggaa
      481 agattagggt gtgggagaaa taaatcgtaa cgttcatagc tagtattatt gataataata
      541 cttttagctg gagaatgaaa tagtcatttg atttttccct tcaatacaaa taaccaaaag
      601 gattcttgtc atgtaataca tgatgaaatt ttggtttggt atatctgctg cgatgttcgt
      661 ctttggtatt gcaattgggt tttatgtagc ggtaaattca tccaaacaac tagctgtatt
      721 aggagcaact ggtgcaatca ttgccatttt tagcggtctt ggaacggcga caacagccat
      781 gaatctatta ttacagtggt ctcgggaacc cattttaaga ttcggaacga cagctcccaa
      841 tagcgaacga gtataccact tacatgttac aaagacaaga ggaaagggcg tggtcgagaa
      901 ttgtgcagct ttcctgacag ttgaggacac aattgttact gactcgccaa cgatttggtc
      961 cctttatgga aaacgacaaa taaatattgg agacgtggac gtgcacgcac gtttgttcaa
     1021 agtcgtaaac ggtgagacca taatatttcc tgtcgctaat gaaattcaag gtctcgatat
     1081 ggaaaatcct tatccactca aacaagtgat cgacaagtgg ctgactctac gaatggtttc
     1141 aaccagcgga aacattccga aaccattcaa agatacaata ggaaatattg tcggttttca
     1201 taagtagaac catgcaacat tgacatgcac gttttgacaa gcgttaattg gacaataccg
     1261 ccaggacaaa tcttatattg ttccgttcat taccgtactc tacatatgcc taaggccggc
     1321 ttcaagtcaa taactgtaag cgaaaatgtt tacaagaaat tttttgatgt ttatgaaaaa
     1381 agcaggaagg aattagaatt aaagggcatc actagtttct cgggttatct taccagcatg
     1441 atggaagaga tgatgatcaa atacgaagcg tttgccaagc atgctccatt cattcaaaag
     1501 attgcaattg atcaaaatcg agttatccta aaggataaca aatgtaacag aatcgtagaa
     1561 gtccttctga aggatcagga gttgcagtgt cttcttgatg aaaaatcgga ctgtgtgcac
     1621 gtaggattcg tatattcttt gcctgaatta tattctataa tcaatagcaa agcaatcaaa
     1681 gtacctcgcc gggtgcaatg aaccagtctc agcgctggta tttcatagaa tcttcactgc
     1741 tcgacgaggt tccttaatgg agggggtggg attcgaaccc acgaacccct aagggacggg
     1801 atcacctatt ttcatcttga gtcctgcgcg tttgaccggg cttcgctacc cctcctcgac
     1861 aaagctcgcg cttatcttcc cctatttcag ggttacgtcg tccatcggtc actatctgtc
     1921 tatcaatgta tctgcgaagc aaatcaatct ttagaatgca ataattgatt tgttatcacg
     1981 aacggtgagt gcgtctcggg tcttggtccc gctttctttt aatccctaaa gttaaagtaa
     2041 ttgtcacagc tttaatcagc ttaagggtag atatttcgtg ccgaagcctg aaatcatctc
     2101 taaactatgt acgaaatgtc attcaaaaac cgttcatgaa ataagagaaa agacggttgt
     2161 tggaattaga cttgtttgta ccgattgtgg acaatcgatg atgataagca agatgtaatc
     2221 taagtaatcc ggaaccttta ttataacaat cggataaagc aagggagtaa gaaatgaaga
     2281 tcactgatct aaaacctgga atgcgagggg tcagcgtgga cggaaaaatc gaaagcatta
     2341 cagaaccgcg aacggtaaat ctccgaacag gtggaactgc tcaagtcgca gatgcgatgc
     2401 tctccgatga gacaggtcaa ataaagctct cattatggga tgatcagatt aaccttgtca
     2461 agcagggaga tagcgtttcc atagaaaacg gctacactca agcattccga ggagaaaaca
     2521 gcctcaacat aggtagatat ggcaagatca aaaggttatg aaaacgttcg tcatcttttt
     2581 caatagcatg agccaaacca tgctttatca gtttggtgtt acatccaaaa gcagcgcccc
     2641 aaagagctgg atgtcttccc gctagctgat gtagcccgta tcagacggtc tgttcgttgc
     2701 gctaccttgt tgttgttgtg gttgtcccat tcttccagcc atttgctgtt ctattgtttg
     2761 gattaacatt tcggtgtctt tggctctctt ttcaagattt ttcatgtcta cgctaacacc
     2821 tacgatcgaa agtaaagttt ctaagaggga tttggatgct tttgcatcga ctacatatcc
     2881 ggaggtttct cctaaaaggc aagtaccctt aaccccccgg agttttgcgc taccaatgat
     2941 aagcccattc ataccagtga tgctcccgcc gtccatcgtc gagatattac gctcgccaaa
     3001 ggactttatg gtatctgtat cagttgcggt gccaaaaatc cgcggatttt ccacaaacac
     3061 tccggtaata tatgctgcca aagtaaaaac gtgcgacgtc ttgaatctcg cagcaatatc
     3121 caatatttcc tcagccaaga gatactcaga atctgggttt gcaggttgag agtcacctgt
     3181 cagtaacagc agactggtgt cggtggagct cccacccttt gagtagaaga tagtgttttt
     3241 catgagatct gcagtcccgt cagatcggat cagaacctgg ggcgggaaag aagtagagta
     3301 aatatctgca agatgctgag catgcagctc ttcgatcaga tgatcgacgg ccatcttccc
     3361 gacgtatcca cttcccggaa aaccacaaat tagataagga gattttaatt ctggtaattc
     3421 gcctgaagaa taaattacat ttatcccagg gcgcgaggct aacataaaat ttcgaacgat
     3481 tcatgcactt taaaacgtta cgagatagac tttccttctt ggaccgacaa aggaagcgaa
     3541 aaccgtcgct agctgtgaat aatttcccaa tagaatgacc tgtgtccgta ttttgaggag
     3601 accggaagag tttggattgg ataaacattt gagttattac cttggatgca agaattaaaa
     3661 taaaaaggat ctaaagtcct tattgtttca attaactgca cactaaaacc agttgtgcct
     3721 tcagataata ttattaatga aaccagttgt ttttcagatg tagcgccttg gtagctcagc
     3781 ctggtagagc gttgccttgg taaggcaaag gtcgcgggtc caaatcccgc ccaaggctct
     3841 ttacgtttga attggagtag atcgtgatcc cgtggggctc tcagatcgtg atttgcagtc
     3901 tcatcttgat agtctcaaga agtcagtcct tgtaaaattc gaacttactg tcgtctccct
     3961 atgtcgaatc catgttgttt ccaaaaagct accgagagca ccttcaaatt acactgaaag
     4021 ccgaatgaat gatgaagtat tgcacatatc ctcggaacat aggacagctc ggttgagcat
     4081 ctttaattct tcatagccac ctgaggggaa tgttgcgtat agtattgtgt ctctgacgta
     4141 gaaataccga tcatctcact gaatgtagcg tcatcatcct aatagttagg aagatacgag
     4201 atatgagata gtatagacgt acgcttttat tatatttcga atcaagtcaa actgctacaa
     4261 aggcgggggt tgtagagcct ggccaaataa gctaactgac tttggagacg cgggacttaa
     4321 gatctcaaga atgggtgatc ccgtctctta ggggtccgtg ggttcgaatc ccaccccccg
     4381 cacgtaaaga tcgtctcatg gagttgctca gatttgttgc gctcgtgtat gcctcttaca
     4441 atccaaaacc gtcttgataa attgcctatg aattctgtca tcaccagata gttctggatg
     4501 aaaagaagtg cctataatat tgccctgtct tactccaaca attctattat taaatttggc
     4561 aataatgtcg acagccgcac cgacctctat tacgatagga gctctaatga aaactccttt
     4621 gaagaactta ttattcttac caagaatcgg tatacttagc tttgcttcaa acgagtcgtc
     4681 ttgccttcca aatgcatttc gttccacgac aatatccaaa ttagatagaa gttgctgtct
     4741 tgtctctccc acgactcgat cgtatgcacg ttttgcaagc attatcatgc cagcgcacgt
     4801 tccaaggacc ggcatacctt cggctatacg ccgtttgatt gtatgaagta tttcgccctg
     4861 tattgtggca agagtactaa taaccgtact ttcaccacct ggtaaaatta agccatcgat
     4921 tttttcaatc tcttcagcat accgaacaaa gtttacgtta ccttttactt tggaacggtt
     4981 gagcgcttct tgggcgacag ttttattttc ttcaatatca ccttgaattc ctaggacacc
     5041 aacagaaatt ttggtcttca aatggacggt ccccgttcct gcatttttaa ttcgaggttt
     5101 tttgtatcca aaccaagcat ggactttttc tcgtcgacca ttttctgagc ttcgacaaca
     5161 atcttagggt tatcataata agcggtagcc aaaactaccg cttttgcacg ctgagctgca
     5221 tcgtctgcct taaatatacc tgaacccaca aataccccgt cgcaaccaag gctcattaaa
     5281 aaggacgcgt ctgcaggcgt agagattccc cctgccgcaa aattcactac aggtagtcta
     5341 ccaagctttg cagtctcttc gactatggcg agagagactc ttaactctct tgctgctctt
     5401 atcaattctt gacggttgtc agaaattgag gcaatccagc ggatcgcgtt attaactagc
     5461 ttaatatgtg aaacggcttc ggcaacgttt cctgttccgg gttcaccttt ggttcttatc
     5521 atagcacaac cttcttcaat tctccttagt gcttcaccaa gattcttcgc tccgttaaca
     5581 aacgggacag tataatccca tttccaaata tgcctgaggt cgtctgcagg agtcaaaacc
     5641 tcactctcgt ctatcatatc caccccaatc gatgctagga cattagcttc tgaaacatgc
     5701 ccaatacgac atttggccat aaccggaata gtgacggcgt tcatgatgtc ttcgattatc
     5761 ttgacactgg cagttctagc tacgccgcca gactttctaa cgtcgtaagg caacttgtct
     5821 agtaccataa cagcaacagc tccggcatct tcagcaattt gcgactgctc aacatttgtg
     5881 acatccatta caacaccgtg cttttgcata tgagcaaaac ctcgtttaag cagtgtggtc
     5941 cctctgagaa ctaccgtttt atcaaaggtc gtggatcgaa ttcccttcgg ctcatattca
     6001 gctgccacca gagatattgt cataaatgaa cttcttctaa cacttatata gatattattt
     6061 tttttataaa ataagtcgaa gtgctaatgt ctgtacccgg tctgcttgac aagccttatc
     6121 gttgttaatg acgttgccga gatgtagatt catttgtaac aggctaactt taataatgaa
     6181 actcgcacaa ctaatgaaga tttcagattt gaaaataaaa cgattcgacg cttcgttagc
     6241 cttatttatg attcctatcg taatcgtgtt tgtcgtgttt cttatgctgg aagcaggcct
     6301 tgtctcaaaa tagtccttag gcagtacatc ctcacttatg ttggggttct aaggtaccgc
     6361 cagccactat ccatatttta taatttggtc gaactacctt acacctagaa aactcataaa
     6421 gtataatagg tttgagcgag aaaaacctat gttggccgag attgctaaaa aaacaaacag
     6481 cgctgatgga gttaattgcg aacactgcgg aaatcatatc gatgcgtgca tgtgtgaatg
     6541 tccatactgc ggcgaagttg atagatgtga gtgttgcctg tttgatgcgg caactggcgg
     6601 atgagattac agtttttttt ccctgacctg tatgatcata aagcaataaa aggaggaaaa
     6661 tgcaaaccac attgtcttgt tggttggagc aaagaacttc gctaattcgt taacctggat
     6721 gataggcggc gcccagggta gcggcgtcga ctctgcagca aatattttct ccagagcttg
     6781 ttttcagggt gggctccaca tcttcggaaa tcgggaatac tattctaaca ttaaaggtga
     6841 acacagctat tttgcagtac gtgtgtccga agacccgatt cgttcccacc tggacgacat
     6901 caatattctg gtttcttttg atgctgaaac aatattcaga catgcggaca aggtaacgag
     6961 tggcggtgcc ataatctacg attcagattt gagcagcacc acacttcagg atgttccaac
     7021 aatagatgaa cctgctgcta aacgtattag agagatgcta gaaaagagtg aaagtccgac
     7081 taccgttaac ggaatggtaa acctcgccaa aaacaatggc gctgttctat atgacattcc
     7141 gtattttggg atacttaagc agttttctgt ggcgattaat gagcccgcac tcagcaagct
     7201 aacacgtatg ataaacgtga tggcactatc cgcatcgatg gccatattgg actttgacaa
     7261 agagatacta gccaaagcaa tcaaagttat ctttaaggca aaaccgaaga ttgctgacgg
     7321 caatgtgctt gcagcaaatt atgcatacga atacgtcaaa tcgcgatttg atttatcgca
     7381 atcccagtta aagttgtcat ccaaaccaca gtcagccgat atcatccttg ttcagggtaa
     7441 tcaatcttca gcaatgggga aattggttgc cggttgcaga ttccagacgt actatcctat
     7501 aaccccggca agcgacgaca gcgagtttct cgaatctaac cagatagtag agcagactga
     7561 tggcaataaa ggatctatgg tagtcgtcca gacagaagat gaaatagccg cgattactat
     7621 ggctattggc ggggcattga cgggcgtgcg atcggggacg gccacatccg gaccgggatt
     7681 ttccctgatg gccgaagcac ttggctgggc tggaatgaat gaggtcccgt tggtagtgtc
     7741 gctatatcag cgcgcaggcc cctctacagg actaccgact agacatgaac aatccgatct
     7801 gaggtttgct attcatgccg gtcatggtga gtttcctaga attgtttttg catctggcga
     7861 tattgaggag agtttctatg acagcgtaaa agctttcaat tttgcagaaa gatatcaaat
     7921 gccggtaata cacatgttgg ataaattgat tgcgaatagc gttatgacat gtaagtattt
     7981 cgaccagtcg agagtggcga tcgatagggg aaaactctta cagagtctac cgccctccgt
     8041 agaagttgga gcggcgggcc attatcttcg gttcaaattt ggggagagcc cggtttcgtc
     8101 acgaattcct ctcgggactg aaaatgggat attctggaat agtggagatg aacataccga
     8161 agaagggcat attactgaag acccttttat acgcaacaag atgatggaca agcgattaag
     8221 caaattagat ctcgccttgg aggaaatatc tgatgaagat aaggctgtat cctacggaca
     8281 actagacccg tcactcgcac ttaccgtgat cggttggggc tctactaagg gtgcaattct
     8341 ggacgcgttg gatgagcttg ccctggaggg caaaagggta cattttgttc aggtgcgtct
     8401 aatgaatcca tttcctactg cgttactaga aaggatgctg ttcaatacta aaattttggt
     8461 ggacatcgaa atgaaccaca cttcgcaatt gggatcgctt gtcaagcaat atctgcaccg
     8521 tgatagcgag tatagaatag taaaatacaa cggcaggccc atgtctacca gtgagattta
     8581 taactcgtta atgagcatca ttaaaggaaa tgctcctagg aggattgtgt tagaacatgg
     8641 cacttaagct tgcggattat aaaacgaatg ttcataatga ttggtgcgct ggctgtggag
     8701 attttggtat tttaagcgca attcaaatgt ccttggcgga tttgcagatc cctatgcata
     8761 aggctactgt attctcagac ataggatgtt ccggcaagac agcgcacttt atccacacct
     8821 acggcatcca tacccttcac ggtagggtat tggctttcgc tcaaggggcg aaactgtgca
     8881 atccagaatt aaacgttttg gcagtgggtg gtgatggcgg gggtcttgga atcggtgcag
     8941 gtcactttgt cagtgctggt cgaaggaacg tggacatggt ttacattatg ttcaacaatg
     9001 cggtctacgg cttaaccaag ggccaggcgg ctccgacatt aaaattaggt atgaagacca
     9061 aatcattgcc acaacctaac gtgaacaatt cagtgaatcc catagcgctg gcgctagtgt
     9121 ctggatttac ttttataggc agaggctatt cctatgatgt gaagcatcta aaggatttga
     9181 tcgcgaaagc tgttcagcac aaggggctag ctttcttgga tgttcttcag ccatgtccca
     9241 cttataatga catcaatact aaagaatggt ttcaaggcgt agataacatg aatcctgaga
     9301 ccggcaagca aatgcctaga acgtacaagc tagaggaaac cggctatgat ggcgttgtgc
     9361 atgacgagga ccagttgaat ggtaaaatgg ggcaggtgat agaacggtct aatgaatggg
     9421 gaggcaagat tccattaggc gttttttaca agaatgaaca tattccaact tatcaagaga
     9481 ggataacgtc taagatccat aattacgcag aatggccacc atctagacaa agcattgcag
     9541 acgagaatca tggcccaata actaccattc agaagctttt agacactctc agggtcgaca
     9601 attagcaatt aggtacagaa gcaagattag gctattgtat gatcttcaat atcttttaca
     9661 catacaaatt tgaaaatagc aacagatatc tagattcatt cgctaacggc ctctgaatcc
     9721 ttgtgtattt gacaggcagg ttctagtaaa aaaagaaaaa gtaaagaaag gtgaggattt
     9781 ttgggtcagt cccatttact ccacgcggcc atccatctgt aagtccacgt gtttagttta
     9841 gcacccaatg ctgctatcgg tacagttagc actgctgaag cgcctgctcc caaggcaacg
     9901 ggactattgt agaactttcc gacctgaggc tcaattggcg tcatataagg tggcaatgtg
     9961 ggcctaggat acttgaatgc gacttccaac ggatctcgca ctagcagcag gttgatcatg
    10021 ttgaataacg gaagtgatag accaaccaag gtaccgccga caagtattag catgtgctta
    10081 ttgcgccgcg tagcccaata cgtaaggtcc aacaacatgg ctgaaggtat ccatactggt
    10141 accgctacga agtcatatgg gtatcctaat gcaaaccacg caccttttgc aacccatgta
    10201 tagatagtca ttatggttgc gtaatacgtg gctgttcctg gaactcctgt aaagagcaag
    10261 taatagacag ctcctactgc aagcatagtc gattgtgaaa tcgaaaatat tacaaaggat
    10321 gtccaagccc agtcggtgta gaagatatag tctcctgcgt taattgtcag tagcgtgcta
    10381 ttgacagcaa ctacaacaat gaacaaatag tgggttgtac gtctaagcca gaccatagga
    10441 ttctacgacg tcaagtaata tataaaattt tattctattt gcaagaggac tatttgcagc
    10501 gtggattact tgtagtatgg tccgtgaata gcaaataata tgtcaaccgt atgtaataat
    10561 aaaaaatata aaagatctga gtgtcaagtt acggacgatg cagtctctca ccagtgatct
    10621 tcattatgaa gaggaatcct aatgattcaa tcactgtcag cacggaaagt gcataaaggc
    10681 cactaggcaa aggatatgcc cacggatata ttgttacacc aacataaacc ccaccagcag
    10741 tcgccgccca gcataatgca aagccaaaaa tatagattct ccgccagaat gaagactcga
    10801 ctctgtgccc tatcatcttc tcatattcgt cgcgacctcc accttctttt cgtccgccgg
    10861 taccgtatcc ctttggtaaa gtcatctaat ctaaagaatc agtgaatacc cttttaagtt
    10921 ttgctgttaa gccttctcgg attaatagca cggtgttttt acaaattgaa gttgtcgaga
    10981 aatgtattct tttggaaata ataccttcag ccgcaagagc atcgttggat tagacttgaa
    11041 agaaataaaa taaaaataag ttggttg
//
LOCUS       DAOR01000276           10382 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_34746, whole genome shotgun sequence.
ACCESSION   DAOR01000276 DAOR01000000
VERSION     DAOR01000276.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_34746"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3244 a         1950 c         1868 g         3320 t
ORIGIN      
        1 gttacctgct tttgacatat tacccgctcc tgttttattc atcatgctac cagaggaagt
       61 catattactt gatttcatac tcggattgcc gctcgcgttt cctgttgaat tcgcaccaag
      121 taccgaagaa gcagaacctg cgctggctaa cataatgcca acagacaggg ccagagccac
      181 tcctaagagg ttagaaacca ttttcgccat tggccataga gagcatacat atgctacaat
      241 gctttctagg tttgaatatg aaaccagaat acatattttt cgttattgat tccgctgcgt
      301 acttgaaaca aacaataatc atggaaggaa tacactccaa atggtatcta ttaaacatcg
      361 gtttgtggta catgtagcat gaaccagcca accaggaaat tataattgct aacaatgttt
      421 atttattcca aaaacttcca tgaatacagt gtatgcgtat tagatgtgct gactgtgaat
      481 gcttaccaga agattgcaaa aagagcagat caccaaagga gtgtccaaat tgtagttgga
      541 aagagtgttg ctgctggaat aatattaatt aaccagtagt aaatctttgc ccgaagattt
      601 atccctccct agtctagttt ggctattact agtaccatct gtagtacagg tatatttagg
      661 ttaacaatgt cagttcttta catttgatat tatactttca aattcatcag gtatttcaac
      721 atctatgctc tctcccaagt acttctgtga atggcatatc gaataccttg cttgttattg
      781 ttcaaaagcc tttgggggca tgtacaacaa tgattcatgt gatctgttct attatccagt
      841 ctttaaattt tgtaattgca aattcctcag attgtttaat aaatgcagga tcttctatct
      901 tccattgatc ctgagggatg gaatcaaaaa actcttgcat acacagagta aatctaaaac
      961 gtaaattcct cgggttttca attattccgt aacggcgtag gatattagca atttgtccaa
     1021 atgcagcatc ataatttctt ttaaaaggtg ccacttcgaa agtctcgtac attatcgaag
     1081 ttattgcatc tatatcttga tgtagtgtaa tcaaatatga tatgataatc tattacctac
     1141 ataaattggt attatgaagt tcgctctcag gagttggtct tgtcctgtta tttgtatgaa
     1201 atcaagcaat tgtttttgct gatgaaccgt accctccatt tggatttgcg agcgtttcat
     1261 ttatgggatt atcatcttat ttggttttaa ttggtatcta ttcttcagcg atttctatat
     1321 ctgaagactc aaggttacgt aattcaattc gaaatttcgc tttgaaggaa acaaatttgt
     1381 tagatagtat cggctcagcc catatagagc aagagataca aaataaggta atagaattca
     1441 caaaacgtcc ctgctgcaat ataagttaat tcgttagata tgactttaga gccaagacta
     1501 tgtttctgac ttccttaatg catccattaa agaactctta ctttactgat gatggtagtt
     1561 cgactaaggc cgggatgatt gagtacatat tcgcaaacag gccccgatat ttgcttattg
     1621 acgaaattga taaaacggcc caaaagacca agcattccta ctaaacttga tggagacaga
     1681 actttaacgt gggcccaagg gttccagcag tgaaatacct tctaaaaatg ggtttcaaat
     1741 aatcataaaa cacactaaac taaaaaatct ttctatttgt ggctttgtta gcatagtgtc
     1801 ggattttcta gactattaac cagtagtagt tgtaggatag tctacgaaag aatcgagagg
     1861 tgcgcacttt caagtacttt tcctcggaca ttattgttga cttctgaact agctcacgtg
     1921 ccaatatctg tagctactcc ttgaacgact cttatgatta tttcattgat attcttcttc
     1981 ctagaggttg tttccctctg cagtacaaca cgacccttta cataatagaa taagttaaat
     2041 gggatatcaa atttagatgt atgtaaatgg aaattcttta tgattatcca ctttataggc
     2101 cgccttctga agctaattca ataatatttc agattacatt aggttgttct tttaacaaat
     2161 gttcgttttg taatatgtat cgtaccaaag aatatgtaga aagaccatgg gaggagataa
     2221 aatcagaaat agatttagct gccaatgcat atccggaggc tcaacggata tttcttgccg
     2281 atggagatgc actgaacgtt gctactgaca aattgattca aattttggtt tatttatatg
     2341 ccaaattcag gaatttgaaa agaatatcat gctacgcaat gccaaaaaat atattgcaaa
     2401 agaaagacga tgagttggat aaattaagag ccgctggact tagtatgctc tatgtaggaa
     2461 ttgagactgg taatgatgct cttttgaaga aagtaacgaa aggtgcaaca agcaagggaa
     2521 ttattcagag ctgtcaaaga gcaaaacagc atggatatat tttatcatgt atgataattc
     2581 tgggacttgg tgggaaaaca tatactaagg aacatattcg tgatactgat agaatcataa
     2641 gcgaaattgc tcctgattat ttaggtgctt tgacattgta tttagaggat ggaatttata
     2701 atgagtttat gatcaaattc ggtgagcctt ttaccgcatt agacgatatt gaagttctag
     2761 aagaactcga aaggcttatt ttggatttta atcctgtgac accagtaata tttagggcaa
     2821 accatgcatc aaatgtatat tcatttggtg gcactattcc acaggataaa cagaaattac
     2881 tctctttgat cagtgaatta aaactccgcc cagaaatgct aaaaccaaag attttacgaa
     2941 gattctagtt cgtttttcat ttcagagtaa aatcctatat tttactcttc caataggatt
     3001 tatacattac ccattattga atattaatac tctgggaagt accacatcag tttaactcat
     3061 tgaaattgta tgacaaaccg ggactgatgc ttataagccc gctgtcattt gaccacaaac
     3121 tcatttccaa caactgattt aaaacaacgg attaaaaggc accacataac tttgggtttt
     3181 gtcattattt ttttctatta ccattactcc cggtctatga agaatcgctt tgtctgacat
     3241 accaggcagt ggcaaaacat acttgttagg aaagtattca gctacatgac taagcaaata
     3301 tgttttacct gcctcagtat cttttgatct ccattagaaa gtccgattat actcgaagat
     3361 tcttctctac tttagatatt ctaggcgcgg ctagggaata atcagatatg cttcatgcat
     3421 cagttagaca ttgacacacg gtactttagt tttaaagcca tcgaattgtt tgcggtaaaa
     3481 aatgcagata ctcgattttc agattatact tattcttgtc aaactaaatg aaaggaagtt
     3541 tccctgtctt ttatctatag taagggacag ttaccttttc tctaggttta ccttctcttt
     3601 gagtcttgat tttctttact ccttcttcga agtgacgcat catcacgtga gcttctgaag
     3661 catgttttgc tgcttcttct ggtgttggat attttgccaa gtattcatgt agcacaagtg
     3721 aaacggcagt atttgcaacg gaacctgtgt cagcaccact aaagccctcg gtcagttcag
     3781 caaccctagt tattctacat caggaccaat tggcttgtcc ttcgaatgaa tctctagaat
     3841 cttttgtctt gccgccttat caggcatagg cacaaataca atcttatcga accttcctgg
     3901 tctgagtaat gcaggatcga tcatatcggc cctgtttgtt gctgctagaa ctacttcacc
     3961 agacaatgct tgctgttgga gagaagattg aattgggtat tggagcgaca gcaactgcaa
     4021 gaaagatagc aatcgcactg ataaaatgaa gctgagtttc tagcatttcg cctgattata
     4081 caaacatgac ccaaggctgg tgcagctaga cattgcatcc gtcatctcca ctaacatatc
     4141 atgtttatac aatcatatgt gctttctatg gaaacaagag caccattttt cgttgcttct
     4201 cttgaaattt tatgtgctat ggacgataat ccatctagct gtggtgagaa gatgtcgtca
     4261 ggttggtgtg tagatacact aattatgaat agatcgaaat cctcactgcc aaaatcaagt
     4321 gctggtttta gtccagctgt cttttctgca tattccatcg ccttaacact aatatcatat
     4381 ccttatgtgt caaatcctct atctaataca tatttagcga caggcaatcc cagctgtcct
     4441 aatccaataa ttagtattgc tttatgggga gtaccccaca ctaataatca tataaagata
     4501 gtaccaccaa tcactttctt atgagttata taccctaaat ccaaaaacca tgatataaac
     4561 aatctttctt ctttcctgtc atatttctgc aattcaaaaa tgataatcat acctattccc
     4621 catagaacct tcactgcttt cacaccttca ttcgagtctt acacgcgttg atgctcacga
     4681 aagacttgta attctcaact tatttcattt tctagggaat cagattgtgc caactactgg
     4741 tggcataaaa aattttgata tcctcgtcat tcatattacc attattattg gtaacgagat
     4801 tatctaacct ctttaattcc tgattcaagt acgccatttc ttcccttttc tcattcaaag
     4861 atgcatgtta acgattaatc tcaggagacc tagtaggcag aaatacgaag gatagcctat
     4921 gcaaattcta ttaggtacag ttacagggta cagatggtgc aagacaaagc ccctgcaaat
     4981 gaaataaaag atttggtaac agcatttgtc gagaattgga taagaggaag ctctctaata
     5041 gcgtcggaaa tggaacagga tattgttacc tttatggtag atgaaatgaa atacgacctt
     5101 gaaacagatt attatgattt agatcaagaa gacaagcaat ttgttcagat catcaagaag
     5161 ctaatattag acgatagcag cgtgatagtg caagaattgc cagaaatcaa agaaatggga
     5221 ctaggcgacc ctctctttgg tactcaagag tgggtctacc ataaagatat atctaaggaa
     5281 caagtaataa aatatgccat agaatattct gatcgtctaa ggaaagaaga cgaaatcaac
     5341 gatgctgagg actttggata ttcagaccca aattggtata tgaatacttg atctcttctt
     5401 cgtcaagtac tcgaattcat tgtactggtg aacattaata ggcgattcac taactacaca
     5461 catacctcta ttcaaggagg atagacttta agcgtacccg gaagaaatag catgtaggtt
     5521 ttgaagtttt gaagctttat ttgtacctaa tcgtgtaaac atttgcacat ctgatcattt
     5581 gataatgatg attagccaga ttcataccgg tcacaatgca gttgtaatcc ttccgtttga
     5641 gttagccatg gtttactgtc attttctatt tctcctattt catttctaat aaatcagatt
     5701 gtgccaacta ccggaggcat aaacaacttc ggtatccgaa tcgtcgttaa tcatgtaacc
     5761 gttattaatt ttattgaaat cgtctcttct cgttaatccc gccttcgagg atacctgcat
     5821 gatacttttg tcctccaaag gtaaggctct gtaggttatt ttccttgttc tattatctaa
     5881 tgttgataaa ttgtattcct tgttcttcac ctcaatctca agattcctat atttggcatg
     5941 gtacaatttc gattttaaca aattataata ctgctgcaat ttattttacc accataactg
     6001 gacaatgtgc gtacgttaca acatgtgatg caacacttcc aagtaataat ttcttaaatc
     6061 ctgaacttcc tcttgttcca attacaatta ggtcgatatt attttcttca gcataatcta
     6121 ctatgcctcc agctatattt atagaagaaa taatttctgt ttttatttga atgttttttt
     6181 catttgcatt gagtttaact ttattcaaat atttttcacc ttcatttctc atccttgtca
     6241 taaactctga tgtttcatgt ggtccgaata aatcaacatc agcacgtata acatgcaaag
     6301 cataaagttc tgcattatat tctttagata tagggatagt ataatctgct gcatccattg
     6361 acgcatcaga gccatctatt gcaactaata ttttggatat tattgctttt cgactagcat
     6421 cagtagtagt actgggtgtt gtagttgtag ttgacatatg tttattcata aacatgtagt
     6481 gtagatctta taaaaagaag atctattaaa attcatatcc tagaatgatc ataggttcag
     6541 atcctagttc acagttgact aagacatatg ctgttgttat tggcatgcca tgaacatact
     6601 acttttttta catatattat taaagctcta ctacttctcc aggaagtggt ggctgaggaa
     6661 attgactaca aacccacgcc aataatcacc tttacgggtg aacatccaca ttcttataag
     6721 taggaataat ccccaagtga gtactaagcg catttatgat tgtttttccg agctcaaaat
     6781 caaaaagcag acaatgcata cctagatctt ggctttcata accgcacacc aaatagggaa
     6841 gcaatatttg tcaatgcaaa gctgcagaat ggtcgacacc ttgagaaaac catagtgcat
     6901 gagttagctc gccgatatgg ccgaaaaata accaccatcg aaggaaacgg ggaaagtgaa
     6961 agacaaattt gacctgattt cgtaacccaa cccagggttg tgattattcg aaccgaagca
     7021 cggatcaggt cagaattact acgtgatagt aattgattgt agattttcaa ggtggttaca
     7081 gcccatatct ggtacgctgt atactacccc gttatgttca cgtgtccagt gccacttctg
     7141 gaggcatcgt caaatcttct gacaataatg taaggcatac atcccatggc gtgaacactg
     7201 tatccttata gacaacgaaa ggatggtcca ttctttccat tattgagcaa atttggttga
     7261 actttaggtc ttccgttatt gcctctgcgt tttctaatct aaactgatta gcggagatat
     7321 ccaagaaatg atcgatatct gtttgaaatc ttagcatctt tgagatttcc ccgagtatta
     7381 accggtcact gataaattgg ttggattctt ctaacaaaag ttttcgcatt ttattttcgt
     7441 acatcagatc gagaatctcg cccaaagaat cgtctcgacg aaaggtaacg attttctttt
     7501 tcggcattga tgaaatagaa atgcttgttt ggcatttagt accaacttca atcattctcc
     7561 ttgctgagat ggtagaacaa tctccaaatt cgttaagaat aatagaaaaa gcccttcggg
     7621 agtttttcca gatttccatc aagtcgcgga gttttgtttt cttttcaatt agtggaacgc
     7681 cctttaacat gatttctcct accctagttc gatactggta atcacgtgtt gggttttctc
     7741 gtagatgatc caatagctca aaacccccta ctatcccgat tggtttgaag tcatcgtctc
     7801 gaactatgat tgaatctaca aaagaatcta aatattcggc gcacattaaa gtagcaaccc
     7861 agacctcccg tacatcatgg atattaatgc ataccgtgtc agttaatttt cttggaaaga
     7921 gttcttctag ggttagttca tatgccttca tgttcttgga gatagtcata tcacctcttt
     7981 caattacttc actgaaaaag ggcccgccct tcttataaag tttagattct ggactatcgt
     8041 caggcatggc aacgcaagaa ttcgtgtttc ttcggataat agcgagatat ttttctcccg
     8101 gtacccatcg cggcacaccg cattactatc cctgttatac ctagaggctt taacggtaga
     8161 tagaaaatga ctattactac taatactact actcagttcg tcaaagagaa gtaagctcct
     8221 gccgcaagac caccaataac cactattctt ttcttcttat tagtcgataa gtctcggcag
     8281 acttcaatta aggagcttta ttatatcaac tgtattttct atgaaaatcc gtagtccgac
     8341 aacagggatg ttttaaacgt tagccagacc aaatctaatt cgtatcctcg ttcgcatgtc
     8401 aaggcaatta ttgattcatg tataactacg gcctggttgg aggattgaat gatgaaagat
     8461 caaattgatt agcttgattc acgggtaatt cctttttaga cagatcttct ttaatagaat
     8521 ctaatatatg cagaccaagt tcggtcaatc ttactttact atttaagaaa tcatagaagt
     8581 aggaatctcc tcttagtaga atattgtctt tctgcctcat ttcaatagga tgtaattgag
     8641 acgatgatga aggagaagtt gttttatcaa tggcaataac tccctcagac aaagcatagt
     8701 taatctctct tcgctccaag ttatactcaa gtagttcaag aattgaaagg cttgccaagt
     8761 caagaatgct tactattctg acgattactg gatttctaat aagttcagta aggtgaactg
     8821 gctcaattgg catatctcgt tcactcatag gggattatat gtaactggat aaataacaag
     8881 aatgtgtaca ctgcattaca ttgaaatact ttttaatggt ttacaatagt cattccaaat
     8941 tttgttgcta tgccttcttt caagaggact acaataggaa cttgattatt aaaaccaaat
     9001 tctaataaca ataagttatg atctacacaa aataatctac acgatgcaaa caaacaagta
     9061 ctgagcaaat tcattaatag atatggctaa tgttctcaac aatacagact tggataaaat
     9121 agtccaaact gttgcaaatg gcaagagaga taagcaaagt ctaagaaaac cagtgaagtt
     9181 acaaggtgaa tggagtctag atccaagcaa ggactaccaa tttagaacag agctatcata
     9241 tgagaaagga aaagaggtaa ttgaagtaga ttcaccttca tttttaggtg gaaatggtaa
     9301 taggttaggt ccaatggcat attgtgtagc tggtataact tcatgtttca tagctacatt
     9361 cgcaagtgta gctgcaagtc agggagtaaa gctcacgaag cttattgtca atacagattg
     9421 catgattaac tttgccaaga cttttgatat agctgatgaa ccaataactg agggaattaa
     9481 ctttgagata gctgccgaaa gtgagaacgc agataagcag aaacttcagg aactaattaa
     9541 aatggcagaa gaaagatgtc ctgctatgta tagtatgtct catgagataa aggttaatgc
     9601 tcagataaag taaaagccgc ggatatgcac ttgaaaagag agaatgagtt gttgttaata
     9661 gttgcaaatt tttccgcaat cacgctagcg taagaaaaga acaattttaa cggaaggcaa
     9721 tcacaaatat cttaagtaaa tgctattagt atcttaacta ttgcacaaga agaaaaatct
     9781 ttcaaccaag tattgaatga aagaagatca attcgagctt ttaaaaataa tgctgaagtt
     9841 gatgcgacca aactacacaa aattttagaa acatgtgata tgacaccatc ttcgggaggc
     9901 tcacaaacat tcgaaattta ccagataaag aatgaagatt tgaaaagaag gttggtatca
     9961 cagcacaacg aacgtggaag gttcctgatg catctgaggt agtcatattt ctagcttgga
    10021 cttattctag cttcatcaaa catccagaat acgagtatga agtagagaat cacagaagag
    10081 aggatattga actttttgag tttggcagct tattccctat gctcttcccg tttatgttcc
    10141 tcaagctctt ctaatgagtt aaatgatcta ccgctttcat actcctgata aaagccccgt
    10201 tttatgttct cagctaggct gctgacttag tttagatcat tcaactcgtt tattctgttt
    10261 gcactaatac caagtgattc caattcattt agccaccaac catatctttc tggatgctcc
    10321 tcttttgtgg ccataacagt aagacatctc atcggtatat gtaaagtagg attctattgc
    10381 aa
//
LOCUS       DAOR01000277           24616 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20473, whole genome shotgun sequence.
ACCESSION   DAOR01000277 DAOR01000000
VERSION     DAOR01000277.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 24616)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24616)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24616
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20473"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    562..636
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7078 a         5125 c         5010 g         7328 t
ORIGIN      
        1 ttcagctttg tatgcgcttt tgtaggctag taatgcgtcg gacgttattg ttttgatggt
       61 atgattgatt ttgttgtgag ccgtgacttc cgaaagctta tggaataatt gacgcgcgcc
      121 tttgtggtct cttttctgtg aaaggattgt acataacaaa taccatgtat ttgggtcaag
      181 cgcggcaaac aaatacttca tttgaccatc aatttttacg aataattcgt ccacgttgac
      241 atttccgcta aatttcggtt ttagactatt catataatca gaaagtaatt tctcgtatcg
      301 gcgcaaatag ttgtgaattg tttgataaga gactttgata tcgaaagtct cgttcagagt
      361 agcagcaatt ccgcgcaagc ttacgttgcg gcaatgcata gaaagaatca gagaaatcat
      421 tcttgaatca ctctgcgaaa gccacttcct gttttctgaa ttgtctgccg caatctttgc
      481 agcatagaat ttgttctagc actcgcctgc cacgttttcc gttattaatt atagcaggag
      541 atttacaatc agtacaaagt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcat ttacaatcag tacaaagtgt
      661 aatttcttct ggcgtcgctt tcttcgattt gttcttcaac gtcgtttaga attttggctc
      721 gtaatcgtaa gtaataatca agagcaaagc aatgcttgca aacaccgacg ttatggcgac
      781 gatattcaaa atccttacaa gatatcgata gtcaaattct atggctaccc aaaaatggag
      841 gagatccggg agggagactg ttagttacaa aggtcaatag taatcccttt cgttatgacg
      901 gtttctactc tgcaggttct attagctcat gtatcttaga gtcttcgttg aacaggtaat
      961 ccaggcttcc caaacacaac aaaggtaaat tccaatcaaa tgtttattta atcgcagcta
     1021 atagtaatta gtaatatata atgagcagca gtacttcatg gcataatccc aacgcagata
     1081 acagtaatca aagtaaaata tgtcctaatt gcggacaagt aaacgacaga tacggcttgc
     1141 ctatatatcc tttcaacaaa tcaaccaagg tattgaactg gtgcacaaag tgcttaagca
     1201 cggtagatga tatcacaatc gctgatcgag aactccctca gggcgataaa gcgtaaattg
     1261 gggcgaagat acagttttat gattatttat ttatcacata aaagtcataa atgaagtttt
     1321 tgtctgctct tgacgtttgg cgtttagcgt gatattcaat taatgtccaa aacgccggga
     1381 acgtctccac caatcctaag atgggacaga gtatgagagt ttgcaaatgc aacacgaatc
     1441 tctttgacca tgctatcttc gaatatctga ggtttaaaaa taatcctatt tgatatgaaa
     1501 gaagccatat catagaagaa aacaacaaga tcgcgccaat accaaattga aaagcaccca
     1561 tgttcccaaa aaaatgtaca gtcaattcag aataaagtgt tctcagcaag ttcccaaaga
     1621 cagccatgcc atcagcgcca actctataat tgattaaggt atttgatgtc ataagcaaaa
     1681 gaggtaggtt tatgtggaaa gaaaggatta aagagatgaa gccagaagca aagcccataa
     1741 aatacgtaat cagcttgtaa gagagcttaa acctgtgaat cggaggaaac ttgtttatgt
     1801 tctgtaggct acccagaacc catctacgcc tttgcatgaa gtggtccttg atgcttaggg
     1861 gcggctgctc caaaagaata cctccgtgcc agcccatgga tcttggacca tatttatggt
     1921 acacttcata gccgaacatc tggtcttctg caagagttgg tccgaaattc caaccgatcg
     1981 aatcctcgac gtctgatctt accagtaaat tacttccgtg catgtgcaat ggagaagtct
     2041 ttacatattg ctcatttttc atctgcgata cgcaatcgta gcaagtaaat ggccttatgg
     2101 attcagcaag tgctgttagg atgttcgccg attcaaattt taaagggtag aaaatgggtc
     2161 cttcaaaggc tagtccttga ccctctctaa tggctttcag cagagagaga acagtttgat
     2221 ttgtaacata actttcgtcg tccatatgga agatccagta tttcgaagtg tttcttccag
     2281 ttttgcgcct ggattcgact gcatattgta acgctcttcc tttcttgatt gcattacttc
     2341 tgaagctttt atcaaccagc acaacctcaa tatttgagtg ggacagggta tcaatgtcct
     2401 gtgggtcgtc agtaataacc gagattcggt aatcgatgta ctttatcttg tcacaagatg
     2461 tgactattga atcgatacct cgcagtacga ccggcgtctt tgttgcagaa cgactagtta
     2521 tttggaatat tattaccggg tcgttatgaa ttcgttgaac gtcctttcct atgaaagctt
     2581 ctctattctt cagaaacctg attatagata caaatgcaat agggacgtta gtaagccata
     2641 caactgttat aatgcctact aggatatcgt ctagcaatat ctagagacaa aatacctcat
     2701 aatatataat aaccgctttc tagaattttg taaatgttag ctagacgatt tgttataggc
     2761 acacattaag atactagaat aatcttaaga aaaagatgtc tgatttcgat tttctaagca
     2821 tattgtggtt tatattagaa ttatctaatt tctagaaaaa ctatggataa aaagagttgc
     2881 gtacttgttt cgattggagc tcagaccata atgcctccgg agagcaagtt aaacgaacgc
     2941 aatgatgcga catactgcga acaatgtaag ggttatactg ttatctttga tatggtttca
     3001 agtgagtacg tctgcagctc ctgtggttgc gttgcaaacg ataaattttt taacaatgaa
     3061 cactcgagcg gatattctgg ccaatctggc ttcagcgata gatccagaac tggcatgcct
     3121 gaatctttgg ctatacatca taagggacta tccactctaa tagggatggg ggacacagat
     3181 gcgcgcggta aagcgctgga accagcacag aaactgaaaa tgcaaagatt gcgcacttgg
     3241 aataacagat ctcaactgaa tgactcaata tcgagaaatt tggagaaggc tttaaaattt
     3301 ctaaataatt ttggcgaaaa acttaacctt agccaagctg tgctggaaag tgctgcatat
     3361 atctacagga aagcagcaat aaaaaagctc gcaaagggca gatcaaccct agggcttgta
     3421 ggggcagcgc tttacgccgc gtgcagggag actgcaaccc cgaaaacgat ttctgacgta
     3481 gcttcagtct gtaatttgtc cagtaaagat gtgatgtcac attataagtt gatattgaga
     3541 gagctttctt tacaaatgcc ggtactgcat gggccagatt atgttacact catagccaac
     3601 agacttgatc tgagtgagaa aacaaaacga gaagcactta aaatattttc ggaggtacag
     3661 caaaaccgaa tttcaatcgg gaagaacccc agagcgctgg ccggcgctgt aatatatttg
     3721 gcttcacaaa cctgcaacga gtttctccga caggtcgaga tatgccaagt agctgacata
     3781 tccacagtct ctctccggaa aagatgtaag gagattaaga caaccctaga agtttagctt
     3841 gttaggtatt cacgggatct tcttgatata acgtacaaac tccccgccat catgaagatt
     3901 cattaattgc tccctggcta tctttatata tgcagcctgg aacccaggat atgcttcttt
     3961 gcaagagaac aaagtcaata tcattccttg gtgattgatc ttgaaacctt gacagggctc
     4021 atgtcctctg ataacaagtt tgctggccga cgtattaagc cactttttgg atatcggtat
     4081 cccaaaatgt ttaccatagc ctctcctgga atactcccaa tccaacccat tactaatatg
     4141 ttccatcggg tcattccaca atatttcctc catagcagta ctactgatga acgggtcttg
     4201 aacagcagat ccaaggccat ttccgcgacg tatatttagt aacaaatcgt cccctgtagg
     4261 tagccctccg tgaacgatca aaagttgttt cggtatcatg acgattaaac taagcaaccg
     4321 aaagaatggt attatcttat tgccatagat tgattttgcc attttatgtc cgtacttctg
     4381 agcgagtttc agtggcaaat catgcgaaga aaaaggaaac tccactggag cttcatgatt
     4441 tccacgcatt aaaacgactg agttggggaa tttttgtttc aaataacaga ttacatacag
     4501 cacgccaata gagttgcttc ccctgtccac gtaatcacca agaaatatga gcttattatt
     4561 gggatttgtc agtctagtct caaaatcaat atctttgagc acccatagca atgattgcaa
     4621 gtcaccatgg atatcgccta ttagaacaag ctcttctgga agactgtcca actcaaccat
     4681 ctgggcgttc accttgccgc ggttgatatg tccaatttgc ctttctttct caatagcagc
     4741 cgtagcattc tctacaacct ccgtaagagc ctgcgcctgt acttcaacga acaattcttc
     4801 gaagtcttcc agcaagttag atgtcttgtg tttgcttatg aagagttaaa tattttgtga
     4861 gcaataactg attaggctgg ttacttttca gaataccggt agttcaccgt gcggtatact
     4921 attctctgat tgttttggca ttggctcaga taggaaaaga aagaatctac gagtcatctt
     4981 catggcggat gcgtgttgca tttgaaaaca gctggtccta gttaaaaagt atatttatta
     5041 aaagccactc gtagtgatct aatttagatc ataattgcca ctaaacacaa aaggatggag
     5101 taatagggga tggctagaga tcattgaatt tatactttcc atgtgtgagg gaggcgcacg
     5161 gaagacgcat gtaatgtata gatgtaattt gaattctaaa caaattagtg aatatttgaa
     5221 ctttcttaca gattgcggta tgctggaaag agtacaggag agggctagct cgaagcgcta
     5281 ttttttcagg actagcgagt tgggtagtaa atttgtaggc agatataagc agatggcaga
     5341 gctattcagc aagccgcctc cgccatcaat aatgaatgaa tgatacccta tccttgtttc
     5401 aagtcattaa atcctattag cttaattcta tcacaaaagt tatcagtaac atatataaat
     5461 ttcatatcta tgttctaatc tatgaataat atttctatcg tatacttaca atcacgttaa
     5521 atgaaatgta agtattattc tatcgtcggc aaaatgaaga atcgtagtcg ttacgaagtc
     5581 atagctgcaa ttctaaaatc agcatgtaaa gaagaaacgc gtactaagat aatgtacaaa
     5641 gctatgttaa gcaatgatca atgcaaaata tatcttgatt ctcttcttca aagcggccta
     5701 atccaggaag ttgtcaatgg caataaaatg gtatatagaa tcacgcagaa cgggaatagg
     5761 tttctttcat actacgatca aatgaaagaa ctattgccag tcggagtcga agagaacgta
     5821 gctcaagagt actaccacct tagtgctaac cctatctaaa ctagagattc aagtctgagg
     5881 gctggatatc ctacagatct atcagtcaca actgcataag gatgcgtttc aggcttcacc
     5941 gaatgcagta ctaaggtgcc actaatggat gtgaggtgga gttggacgtc tgccatttct
     6001 gaaaggctga gctctgtgct atgattctgc ctcactgcga gtagagtgag atctgccgta
     6061 gcgctgatga agggtaggag atcggttccg ttcgcttcgt tgtattgatg tatcctgtga
     6121 cacatagcca tccccaatac attcaccaaa agtttgtttg gatagtttcg ccttgtttta
     6181 actatcatat cgcggaagta ttggatttgt ttttcagaat tacccacgtt ttgatattgc
     6241 gacatcgtag gatctgaagg aagctcgtcg tttgtgcctg tacaaaaaat ctttacgaga
     6301 tcttttttgt gctctgttgg cagatatggt cgaagataac tcataattac cttcgcatca
     6361 atcccaccag aagcttcaat taatatggga ttgtcgtttc tgagtatgtt tgagattatt
     6421 cctgctagta atgtgagagt tacctttgca tgtatgttcg attgcgtttc taggagtacg
     6481 atgcctttct tcgggaatcc tcccccaagc gcttcgtcaa gctgctcata tccgctaggg
     6541 acgtgatgac cctgcgttgg tgatggggta tgtattggtg atcccctcct taggcgttcc
     6601 gtctgattga tgttattgtt aatgttattg ttgttatacg aatcactaga cataaagtca
     6661 cacgggtcat aaggattgta gctctgaaaa atacatttgt ttagggtata gatgtaggaa
     6721 ggttttctaa tctggattcc tctcagtttt gtcaaggcga tttctcgtat cctcatccca
     6781 tcatgtagct tttgattcaa ttccacgatc ccatctacca ggaaatcgag agtagtgtca
     6841 ataggatttt cactgataaa aatcaatttc gcacccgctc tttcccgcca tgtttgcaag
     6901 actcgttcat tattcagacg ggcttctttg tccataaaag atgcaatagc atcccaacta
     6961 tcgataataa ttattggtgc cttaacgtcc atcaactgat ttgtaattct ttcaaagagc
     7021 gattcgggtt catcgagccg tgcatcctca aagcttgaga ggttataacc tattcgttgc
     7081 gacgatgatg ccagtggtgg cgtttcatcg tgatttgggg ctatatccac atatctacca
     7141 agccacggat agtagacaaa aagttgtttt ggagatagcc tcgttgaaat ataaaagaag
     7201 ttgtttttga tctttaaggt tctcagaata gtaagtgaga gtgtagtttt acctgctccc
     7261 gcatagcctt taacgagtaa cgaatacgta tcgcgccgaa caaattccag cagctcgtaa
     7321 gggatatggg ctgctgttct tctgctgctg ttagtgtcgt tcccaaacct gcctggtcgc
     7381 ttccccgctc tcttttccaa tccagttagc acttccatta cggtaataat aaattctttc
     7441 taattttggt agaaaatcta cccgccaaaa ctcgatcatc atgtgacaca gttctggcag
     7501 caatggtaac tgtaatggcc acggaagagg gcagttgtgt ggctcaagaa acgatatcta
     7561 ttcacaatgc cttagagcag ttacggcatt gttttgtggc cctagcatca aattataatt
     7621 accacgcttc gaatccgtcg ctgccgtgaa gataaatgaa acctgcttgt catgccggag
     7681 ttccgatcga gagaattgct cgctaaagtt acccagatct gccataaacg tccttaatgc
     7741 tgcatatcaa tagtttttta tgtaggcagg tagccgtcaa aaaacataca ttggctcagg
     7801 cacccttaat gccctgcgcc acaagtactg aatagcaccc aaggccacag tcttcacata
     7861 taggctttaa cgcgtcaccg tctgaactac ctatgcaaca cggctgcttc acctttccca
     7921 tgtgatctaa cgagagtatg gcccaggaag gacactgtac aggtgtggta cccacccctc
     7981 cccagttacc tttcatcaat gatagttgct tgacaccatt gaccacgaag cccggatatt
     8041 tctcttttag cgcaataagt ttgtcgacca cttggctacg tttctctcca aatggcagcc
     8101 acaaagggtc acctttaata aatggagtat gaaattgaaa tcctatctta ttcacttttc
     8161 ctatccactc gtgtaccaga tcctcaacag cgaagtgatt taatgaatta attgtcatcg
     8221 tgatccatat gtctttccag atgggttttc ctcctcggtg atcaggactt cctgcgatat
     8281 aatccaaaat attctgttta gttttcgaat aggaaccttc tcctctaata ctatcatgta
     8341 ctttctctgt cccatccaaa gatatccaat aaaagtacag attttcaaat ctctttagag
     8401 ggtatgttcc attggtgact acacaaacgc gtttaggcat ttcctcgcag aaaacttgaa
     8461 taacgtcagg acgcagagtc ggttcacccc cgactagcgt cacaacaaag aggtgatgct
     8521 ttttgaaagt attcttaatt atttctctcc attcttctgg gctcaaatcg ttgttgttat
     8581 tcttcctatt caaccaccaa taacaatgtg tacaatgcag attgcacaca ttaatgatgt
     8641 ctacagagcc gtacatcgga ggttttgcct taaaaagttt gatgctagca tacttcgtaa
     8701 atatactaat atagaagggc atttcgcgaa gaagatcagt tagaccacga ccatagatta
     8761 gatctaccac acagtggaat aatagaacaa gtaaataagt ttacaggaga ttatattctt
     8821 ctcttctctg ttcttaagtt gatcatttaa aagtgaattt ggctagtctg tatgttgtct
     8881 ttactgcgcc tatacctaat ctaacagcta gtgattgact tgcaagaggg atcagcatcc
     8941 tattcgcaat atccctacat agcaatagaa cccgactcca tggatgcatg atagtggcgc
     9001 tgttagcttc caacgcaaga agtccgtcaa tcgtagtcga gaagtactca gagtaaaaca
     9061 gcatccgcct ctctcgtgtc ctttcaacaa atccttttaa cattagaccc gttattacct
     9121 gggaaatgat tgaaacattg acacctaatt gtctagcgat cttcttctca gtcttcgagt
     9181 tgacctttat tgattttaat acttgatatt ctaatctcag caatagccag gtctggtcgt
     9241 cgatctcgat gttgctcaac ggcacacgta aatggttcaa tagtattatt taatttatcc
     9301 gtttgttgat ctcggtcagg taaatatatg aaaatctaac aaatcgtcaa acgatgttta
     9361 ttaggttttg aaaaatctag acatatatgc aagaaatatc ggtggtgaaa ttactggtct
     9421 agtcccactc gtgttcgttg tataattgtg catgtcaggc cacaaaacct atggagttca
     9481 aatctaaacg ttggcccttt aagaaccaaa tgattcagat ataaaactaa cccgacattc
     9541 cggcattttg gatcaataat atcagtatca gtttttatct agatggcatc tgaatttttt
     9601 ttatcattgt atcagtatgg ctgacaaaaa aattggccaa taaacaatct ttcgccaaat
     9661 tcggccttca tccttaagta cgattattta ctggacagtt ttgtatggcg tctataaaac
     9721 tagcagtggt ttctaacatt gtattggatt cgattcgaga cacggaaggg attgtgactg
     9781 aaagccttgg aggtcccgca ggctactgcg gtctgactgc tagacgcttc agtttcgatg
     9841 ttagtctggc tacaaaaata ggaacagatt tcccaccagc atacaagcgc ttcctgctag
     9901 ctgaaggtat tatgctaacg gactaccaat tgagccatga tcgcgacacg acaagattcc
     9961 gactcacatc aaatagggac aataataaca taaggagttt gtttttgtcc tcaaaatgcg
    10021 cacagatcac catagatgat atagaaaaaa tagagacgga ttgctggctc gtaagtcccg
    10081 tgatcgatga agtgcctgaa gatgtgctca gagcgataat tctacacgga ggcagaagag
    10141 ggttcgttat ggtcgaccca caaggttata ctagaattgt taattcatca ggagaaatca
    10201 catcagtcaa taaattatct ttgaacctat caggggtgag tgcaataaaa gctgacaaaa
    10261 cagagctagc actccttacg ggtggtctcg aaggacttcc agcaatgcag catctacaat
    10321 ccagaactgg gataaagttt gtcatatcaa caggatatcg tattatccat ttgttatata
    10381 acgggatgca ctattggata caactcaaag atattgagtc gccagattcc actggcgttg
    10441 gtgatattct gtgttcagca ttttgttgcg cttacataaa ggagaacgat cctttgtggg
    10501 ctttttcctt tggggcaggg gcagtgcggg ctgctcttga aacaaggaag atcggattgg
    10561 ataagattcc ctcgaaatca aagatcgaac aaagtgcttc ctattactac aatactatct
    10621 cgttccggag ggcgtaagtt attaacttta aattaaatta ggtcaagtga gtacctaaag
    10681 aaggccgtag catgaaaatc gctatctcag cagcagattc cagagataac gccttactat
    10741 ctcagatgaa aacgactcta gaatcgctaa atattaacat agagctaatg agagattcca
    10801 accacaaact agcgaacgag gtagatctcg tgttagcggc tgggggggat agaggtattc
    10861 ttgattactt tcacaaagtc gtaactgatt cagcaccagt attgggagta tatgaatcag
    10921 actcgactgg atttctcgca cagttagatg tcagagatct ggaatccgcc gcattgagac
    10981 taaagaaggg tgactacgaa attgacgaag tacttcggat agcggttaaa gttgatggca
    11041 gggaggttga acctgttcta aatgacgtgg cagtattttc tagtaaaagc gcaatactca
    11101 tggaacacgt tcttagaata gataacaaag atgtgtggca tgacaatagt gatggcgtaa
    11161 ttatagctac ccccactggc tcaacagcgt actctatgtc tgcaggtggg cctatggtcc
    11221 ttcagaggtc gcgcgtattc attgtagtat cagtaaattc agtggataac acccgccgtc
    11281 ctctggttgt tccaaacgag accgtattga agatttctaa tattatgagc agatatcact
    11341 gcgaagtagt gcttgatggc ggttccagat ttgctatccg tagagaactt gagtgccaaa
    11401 agcaccaatt tcctgctaga tttgtcagac taagggctga ttctgcagca atatcactta
    11461 ttacaaagaa agtcaaattg gcagaggact tgttgagtat gccacctagt gcgaaactta
    11521 ttttgaaaac tctagaatat gagggtgcgc taagtcagag ggacttgact accagaactc
    11581 tgctcccgga aaggactgtg agactttctc tcagccatct gcttggccgg ggatacgtga
    11641 agagaaaaac ttctctacgg gacgcacgac aaaaaattta cgagctcagg atctgatgat
    11701 gccggctgca gcggtcgacg aggcagcgga agccctcacg aatgcgtcaa aagcttgctc
    11761 tggatttccg ggcctgctat taaattccga atgatactgc accgcatagt agaatttgtg
    11821 ctcggggatc tctagaattt ccatcctttg gccgttgtcg gaatgacccg agaatactat
    11881 tccacgcttt tccagtaact cgcggtaatt ctgattaaat tcatatctat gcctgtgtct
    11941 tctgtatatt gtaccggttt tatagatcct ttctgcattt gtattagaat gaatggttat
    12001 ttcgtgactg cccagccgca tcgatccacc tgcgtcatga gctgtttttt gttcaggcat
    12061 gaactcgaca accgggtctt tcgtaccggg atctagttcc gtagaattgg cttcttctag
    12121 accgcatgcg tatcttccaa accccactat agccagctga aatccaaaac atatccccag
    12181 gtacggaata ttctgtactc gagcaaaatt tgctgcccta attataccct cgctacccct
    12241 cttcccaaat cccccaggaa tcaatatacc atcaaatccc ttaagataaa acaggttgtt
    12301 ggtgttgttg ttcatgtttt tgatgttatt gcggccttta tcattgggat gattactcgg
    12361 accgttttca aatttttctg attcgatcca ctctatgtca accttttttc ctatgtgcgc
    12421 tgcagcatgt gatagagcat ggtagacgct gacgtagcta tccggtagtg tggcatattt
    12481 tcctacaact gcaattttca ctaagcatgt agaagcatag aaagattttg caatggatct
    12541 ccactctccc catttaggag aacactttct taacaataat ttcttgccaa taactttgat
    12601 catgccttgg ctcgccaaaa cctccggaac cgtgtagact gatggagcat catgacagga
    12661 aatgacgcag tcctcgttta cgctagcaaa cagtgatatc ttgtgtcttg cttccgctgt
    12721 gagtggggtc ttgcatctaa tagccagaat gtcaggttga atcccgattc ttcgcagctc
    12781 ctgcacacta tgctgagttg gcttagtctt ttgttcgccc acaacgtcca gtactggtgc
    12841 aagcgtcaca tgtatgaata aggtattaga ctctccatct tctaactcca tttgccgtag
    12901 cgcttcaaga aaagggagac tttctatatc acccaccgta cctccgcatt ccactactgt
    12961 cacatccaat tcttccttcg cagcaatttt tcgtaactgg ccttttatgg catcagtaac
    13021 atgcgggata atctgtacgc attggccgag atatctacct tctctttcag cctgaattac
    13081 gcctgaatat acccttcccg ttgtgaggct gtgttctctt gtcagggcaa gatcgagaaa
    13141 gcgttcataa ttgcccacgt ccatatcgca ctctccaccg tcatcggtta caaaaacctc
    13201 gccatgtgca attggattca ttgtccctgc atcatagtta acatacgggt cgatttttac
    13261 acaagaaacc ctgaaacccg ctagctgcaa taattttgcg gtcgatgaag ttacaacgcc
    13321 tttgcccagg cctgacatga caccgccagt aatgaagata aacttccgtt tgatactact
    13381 attcttaacg gcgctactag tgttattaat actagtagca ttaacctcat tactaccatt
    13441 atcacccctt agactacgat ttctcccctt cgactgcaca ttagattttg tagaatcacc
    13501 tttttattct ttctttaaag agaattttgg tgattcatcc tttattcatg gaagaatgac
    13561 cgagacgcta aagggtggat gagtctctct gttcctaagg ctgaagaggc aattcggtct
    13621 cccacccgag aagtatgaaa agcaattata gtattggttg ttagtcttgt tatttttttc
    13681 ttactcttct gagttctttt tagctttcag gctgaccgat atacccgact gctctaaaga
    13741 acgacgtcaa gtggttcacg tactcgtttt ccctatttgc actatacatg gcatttgcga
    13801 gcttagtctc ccttacccaa ccaattttaa tgagatcgct tagattacgt ttgatatcct
    13861 ctcgcttcaa gtttgttctt ttatggagtg catatatggt tgccatcttc tcggcctctg
    13921 ataaggctct tattatcttt atcttgccta tgctgccgag cccgatctct ataatttcct
    13981 tgctactcat tccacacggt cgccattttc accaatacca tcgctgcgac tccggtgatc
    14041 gttcctccga ttgatctgtc tttgtaacat gggttcaagc tcatttagag attttacgtg
    14101 cattccgatc tcttttaatg actttatttc gatgatattt ttggcatgca gatcatcaag
    14161 atgatcctcg acatccaatt tttttatctt aaattcttca gcaagctcgg aagcagagag
    14221 acgaatttct ttgagatcaa catactgcct tttctttgtt cgaagtgccc ttattagcgc
    14281 gagcagcgtg agtatttgat tcttcggtag ctcttcgata tctggggtcg ttatggaggg
    14341 atgaatttgg ccatgaacct ttcggatgtg atcgacaagg accctgcctg ttcctcgaga
    14401 ttctgcaaga tttcccgcgt atagcagaag gtctaatgcg aaccgtatat cgccattgac
    14461 agagggagaa gttgtaaact ttgatacttc gtctatgata tcagacccta tagcgtttgc
    14521 attaaatgct tcggagcatc tgctaactaa tatgtcactt acctgcttga tagaatacgt
    14581 cgtaaattcc attgggactc tgcccagcgt gctcaattcg gcgctgtcaa gtttgctata
    14641 gaaatctgtg cttcttgcaa taaatataac tcccttcacg ttgcatggtt tgtcagggtc
    14701 aaattcatta aggcgagtta gatcgtaaat gatcccgccg ctatctttgc taattttaac
    14761 aagataatca atttcatcca agatgataat cacatatatc ttgttttcgc gcaagtaccg
    14821 caataggtat cttaacattt cttcagcact cataccctga gcaggtaact caggcgctac
    14881 tctttctaat aagagccggt agatcgtata tttgttccca ccctgcaact ttaggttcac
    14941 gtaaccggtt acgagcttga gcctattctt tctgaattct tcctcaaata atttcgagaa
    15001 tttgagaaca gtagaggtct tccctatacc cgcgggaccg ataatttgta atactgttaa
    15061 ggggaattcg tcaggcttag aataagatcc ctttagagca tattgaattt gttcgatctg
    15121 ggtttctctg tgtgggagtt cagccggtac gtatcttgaa gacaattttg atctgtcgcg
    15181 aaatattggg ctgctgaact ccattgctaa tatggataaa gatctattca agatatatat
    15241 tatattatag taagtatttt tcagattaaa attaatgaat tctgttccgt gattcatgca
    15301 tgttcccgag tctgcgtatg gtctaaatgt gtatttttta ttactaaggt gtatttgtcg
    15361 ataaagttga gctcgaggaa aatttgatta tgctagtgtc attggactaa gcgagcgctg
    15421 agtggttgtt gtgaggccag tggaatgtta atatgaagtc atctaatagc tgaatattat
    15481 aatttagtat atgagcagat tcctgaaaac agaaaactac ctaaataagt tagctgaaaa
    15541 ttcttacaaa gctatagatg aaggtgttta taacaatgca aaatatgatt attgcggcca
    15601 tgagccgata agtatgagga aatcaattct gtcgtgccca catgctccgc taataacaga
    15661 gataaaattt gcgtctcctt ctaatggaag gattgttgct gagaccaatg acgcaatctc
    15721 ccgcccgata gacctagcaa ccaatatggt ggggtcaggc gcggttggat tatccatagt
    15781 aacccaacct aatttgttta gaggatctcc ggaatacttg ggcatgattc gcaagacagt
    15841 cgctgttccc ctgcttatga aagacataat tgtcagcgac attcaaatcg attctgctaa
    15901 acgaatggga gcggattgca tattgctgat taaatcaata ttcgatagca acctaactga
    15961 aggaagtata gagaaacttg ctgattacgc aaataaaaag ggggtgcagg ttgttttcga
    16021 ggtgcataat gaacaggaat ttagagaagt attgaactcg ttaatagatc caagacaaaa
    16081 tttgattggt atcaataata gagatttgaa cgacttaaat gtggatattg ccactaccat
    16141 gaatttgctc aagaaagtcg ataagcgagg acacatcata atcagtgaaa gtggtataag
    16201 caaagcggaa gaaattcaac aattgaaaaa ggcaggagct gacgcattct tgataggaac
    16261 aagtataatg gaatcgagtg acttagcatc taaagtcgcc gaactatatc tttctgtttg
    16321 ataataatgt agatctttgt gcctgttgtg ggcgcgttgt gcgtagttgt ggtgttggta
    16381 cacacattat gaattcatgt gtctctagat ttcactaaat cccgaacatc ttaatacacc
    16441 taggacgaag gtttctgctg aacatgctaa gtaattaccc cgttaatgcc agatttggtg
    16501 actacggggg aagatatgtg cctgagactc ttgttcccgc gatagaggag cttgagagag
    16561 cctacgatcg gtacaaaaac gatacagatt tccgaaaaga actccgatat tatctagaac
    16621 aatatgctgg tcgaccaact ccactttatt tcgctaagaa tcttactgaa tacgtagggg
    16681 gtgcaaggat ctaccttaag agagaagacc ttcttcacgg aggtgctcat aaaatcaata
    16741 acaccctcgg tcaaggtctt ttggcaaaac ggataggtaa aaagagagtt attgcagaaa
    16801 cgggtgccgg ccaacatgga gtagcaactg ccattgcagc tgcagtattg aggctgagtg
    16861 cagagatcta tatgggctcc aaggacatga aacgacaaag gcctaatcta attcgtatgc
    16921 aattattggg cgcagcaatt catcccgttc agtctgggag tcgcactctg aaggatgcaa
    16981 ttaacgaggc cctgcgggac tggataacaa atgttcaaaa tacttattat ctgataggtt
    17041 ctgcggtggg acctcatcct tatcctgtta tcgttaggga tttccagagt gtaattgggc
    17101 aagaaatcaa aatgcagatg gagcaattat caaatcggcg tgagcatcct gatgcagtcg
    17161 ttgcgtgcgt aggaggtggt agcaatgcaa ttggtacgtt ctaccctttc gtagatgatt
    17221 ccgatgtttc gttatacggt gtagaagcag caggcaaggg aattaaatct gggaaacatt
    17281 ctgcaacatt ggcagcgggt tcgcacggaa taatacacgg gatgtttacg taccttttgc
    17341 aagaccagtt cggacaagtg tcggatacac atagcatttc tgcaggccta gactacccag
    17401 gcgtcggacc cgaacatgca ttattgaagg acatcaaaag agtaaattac gtaaactgta
    17461 tcgatgacga tgctgcagaa gcatttctgc tgctatctag acttgaaggg ataattcccg
    17521 ctctcgaacc ctcacatgcc atatcatatg ctatggaact tgcaaagaac atgaacagaa
    17581 atgaaaacat tgttgtaacc ttgtctggac gaggggataa ggacgtagac gttgtgcagc
    17641 gatatctaaa gatgaaggaa aagaagaacg agaagaacag caaaaagaac aggaagaacg
    17701 agaaaaaaag aaatgatgaa aagaacaagt ttaggaaata ataggctgaa tcggaaattt
    17761 caagagttgt ccgaaaagaa tgaggctgct cttatctgtt atgtagttgc aggatatcct
    17821 aacattccta caacaaatga aattctgtca tcaattgtca agggaggggc agatgtaatc
    17881 gaaataggaa tacctttttc agatccgatc gccgatgggc caactattca ggaggcatca
    17941 tatcgcgccc tgctggcagg agtcacacca gaaaaatcgt tggaaatttc aaagaatatt
    18001 agaaaaaaat atccctcttt acccgttgtt atactgactt attcgaatat tttgcaaaga
    18061 gccggctttg aatattttat gaagaaatcc aaagaatgcg gagttgacgg gtttatactg
    18121 cctgacatgg caattgaaga atcagataag tatgttatgg aggcgtctca attagatctg
    18181 gcaacaatat ttctagcatc tcccaatacg accccaggga gacttgaagc cattttagca
    18241 agaacctcgg gtttcatgta tgttgtttct gtatatggga taacaggaca acgtaaatct
    18301 tttgaagatt acagtttgac tgctattcgg aatgtcaagc aagttgtagg atctcggatg
    18361 ccagtagcag ttggtttcgg aattagcagc ccatctcata taaagcccat gataagagca
    18421 ggtgctgatg caattatagt tggcagcgcg atcgtgaaca tcataagaaa cttctctgga
    18481 aacaagagaa aaatgctcca acaaatagag gtatttgttc gagatctgaa aaaagcatgc
    18541 agacagaact atttgctgcc ttaatcttta atctacatat tcccatcatt tgagattatt
    18601 tgaacctcta agtccagtgt tacctattct caaagctcat gccacgactt cccgttccag
    18661 tcgtgctaac aacaaaaata gaaaagatag ttacattcac caatggaaac gcaggaagta
    18721 cttcctagtg ctaacccgat aacctaatct cctgggcgaa caaaggatca aattaaatta
    18781 aattaaattc aagattgacc taaaattata gacatccgat cgaatggttg tgtcattctt
    18841 ttgtttgtac caactatata cttctaaagt ccaacgtacc caatatacat cctgaagttg
    18901 gcaattaaat aaatactgcc ttatgagaat tagatactct acaaggatca ccagtatgaa
    18961 agagaaggat ttaaagtatc tctttattaa atcggttcat gccggacata ttgatggtaa
    19021 cgactttttt tcaacagagc gttctggtat tgaaataaaa ataaaagatg aaggctactt
    19081 ccgaaatttt gatctggttg ttgcgataat taaaaaaaga aatgatacag atattcaatt
    19141 tgattctaca gattcttacg atacctatgt aaatataata aggagaacgg gccagctaat
    19201 tcaatttgca cacaaagaaa gatgcagaat agattggatt agcttcttcc ctgtggaact
    19261 aaaatctgat gacgacgtcc tggatgatcg tttacccaac caaattctta atgcgatttt
    19321 gactttcgga agggctattc ttgtgttgga caagaaacac agcgaacgag caaggctaag
    19381 aggtatttta aacttaatac ctgccaccgt cataggctat acaggtaaag aggattattt
    19441 tgaagtactt tcagtatttc gacgattcat aactacgggt atgatagggc tcaacaagaa
    19501 aagacttgtc aagcttttaa gggcgaacca aatcgatagt aacagcggtg tatacaattg
    19561 ctttgaaaat atccaaagga tctgccaaaa gcttgttttt agtcagctat atgatcaaga
    19621 tcccggtttt gcaaaggaag agcttgaatt tgttcaaaaa ctggcagata tgaaactact
    19681 cccggaaaaa aagaatctaa cagagctgat aaggcagaca tcgaatttta agataactga
    19741 ctatctatac tagtaatcgt atcatcaaga cttaagtctg gtcccatacg agcaagactt
    19801 agggcgtaac tattcctgat gtgtcttgtg caaataatat gttgtttaat gtaacttgaa
    19861 actttacccc gtcgcgttct acgataattt ctatgtcgtc gagggaatca gatatgtttg
    19921 ccccctctaa tttttttata actacatgaa cgctctcatg tgatattagt gcctctacga
    19981 agggctgaat ggcttcaaaa tcatttcgtc cagacttggc atgatctttt gattgtatta
    20041 tcaacattgt ctcgccttca taagtgaagt acgccgtcga gtcgtcaaca gcacaagcta
    20101 cggcaaaatc attgatagtc atgtcacaaa ctatttagca aaaagaatca tagagcttaa
    20161 tataacaggt accatagacg atatcacacc gatagaacct gtattcgtca tctccctcgt
    20221 cgcatcggcg tactcagatc ctcgtattaa catggatatg agccctcctt cattacaata
    20281 caaaatattc agcctgtgga tgatggacca caatggcagc agtagattga tctggtatta
    20341 tttgacccgc ctcggttaaa ctcatccctg attttgtagg atccaatatt ctccagacca
    20401 aatgatgttg tgacacgtca ggacagctag gataacccca actataccta agtccacctt
    20461 tgttgccaat cttaagctca ctctttatac gcgaattgat ccactcagcc agagcctcag
    20521 ctgtttctac tgccaatcca tgcaaataat atgcatcagt atatttgttc tctttattcc
    20581 attcatcgat aatctttgtt actttgttgc caacagtgac ggcctggaat gcggcgatat
    20641 cattttcacc aaagtagtca gtcaggcaca ggtttttttc ttttgaggac cttggaaaat
    20701 caaaagatac ctctcccttg ccatcaggca attctacaac caggttgttg tctctattat
    20761 gacatttgaa atagccatat acaaccttag gatcaaacag tctttcttcg ataacacgtt
    20821 ttttccattc ttcaaataat ttctctgact cttcgctcaa gcctttggcg cctcttccgc
    20881 gaaaccccca agaaagcaca aaaagggact ttttattaat gaatttccag atctcattta
    20941 gatctatctg ctcagcttcc aatcttattc ttctagcaat atgaggcgga atgggtgcat
    21001 gaactggctt tatcccgcta tgcggcagtt ttagggattg tgttgcaggt cctcgctttt
    21061 cctcccacct taccaatttc tggtgccatt ctgagacgta agcctttctc tcatctgaca
    21121 tcaacttgtt catgaccgtc agaccttcaa atgcggtctt acagtagaat actccagagt
    21181 cgtaaatgcc tccttctttt gcgattctat ttatgtaact actgttaatc gctgcgccgc
    21241 cgcatagcac agggattgtt attttatttt gtcttgaatg ttccacaaag tactgcatct
    21301 gcttggaagt tgatacgagc agcgcagaaa gcccaaccgc atcagcatta acttccttaa
    21361 ttttttcaac aatactctgc aacggaactt gcttccctaa atcataaact gaatagccgt
    21421 tattcgtaaa tattgtcttg acgagattct tgcctatgtc atgcacatct ccgtatacag
    21481 tacacagtac taacttgcct ttgcttacac cttcccgcct gattaaatat ttttcgagtt
    21541 ccgctacagc cgctttcata cattcggcag atttcaacac aaacggaaga attagttcgc
    21601 ctgcaccgaa tttgtctcct acttccttca tagctggtag taaaacttca tttagagtct
    21661 gcacagctgc ttcgtgaact aattgttttg gagcttcaag gattaccttt tttttcatat
    21721 aactatttac ttcgatgttt tttgatgctg taaggtttgc atgaattcta tcttctattg
    21781 aatcgataac gtcagattct attccatctt ttaatctatg gacaattcta aaataacatc
    21841 tcttacttgc ttcccagctt ggatctactt ctatcctctt actggaagca tccgataccg
    21901 attttactcc ttcgaaaaag gtaatcaatt cagcaagcgc gttaggatga ctgttgaaaa
    21961 taagatcttc ggcgagtttt cgctgttccc gatcaatatc caagtaaggg atgatatctt
    22021 ttgcattgat aattactgcg tcaaggccag cttttatcgc atgatgtagg aatattgaat
    22081 tgactatttt tctggcgttt ggcgttaggc caaaacttac gttgcttagt cctaatactg
    22141 tgaagcaacc tggaattttt tgctttacca atcgtatccc ttcaagggtg gcttttgctg
    22201 attccaagaa ttcctgctct ccagtagcca aggtgaaagt cagaacatcg aaaataaact
    22261 gccaaggttg gaggccgtat tttttacctg tttcgtaaag gaggatcgca gtttctaact
    22321 tttcctgggg tgttttggcc attccccctg gtcctataca catcgttatg gcaggaacgc
    22381 catattttgc catcaacggc gctaacgcat gaaacctact cccatcaccc tcaagattaa
    22441 ttgagtttat gacaggtcta cctgggatct gttggagggc tgcttccata actttaggat
    22501 cggtcgagtc tataacgagt ggtgcatcga tttccaagct taatctttta gcaagggatt
    22561 tcatgaattc aagttcatcg gcccgttcag tcgttgcaac acaaacgtcg aggcagtgag
    22621 caccgtcttc aatttgtgtc ctagctaagt taaccagacc atcataatca ttattgagca
    22681 ccatgtcttt tgctcttctt gacccttgag tgttaagacg ttcaccaatt attagaggtg
    22741 gaggctcttg cctcagatca actgccttca gggcggaact aattctgggt gtatgcttca
    22801 tctttaagat ataacaaaat ctttagcgct agatcctttc taaaacctta cgtttaatgt
    22861 tgaaattatc ctgctacata aggcgagtgt tgtaagaaaa atcatctatg cgtatgcatc
    22921 tttctctttc cccttctcga cgcgtgtcta tttaataatc accctagtac aaatccaata
    22981 ttttgcgcat ctcagcaata tgtaatggat tagtaccgca gcatcctcct attatacgaa
    23041 cacgcttgta attttcaatg aattctttta gtttttcact caatgcggat ggggtcattt
    23101 tgtatacagc tttaccgcct tcattctgag gcatgccggc attcggcacg actagtatag
    23161 gcaattcgtc ttgttcattc aaccatctga cacttggagt catttccgca ggccctgttg
    23221 agcagttcaa accaaaaaca tcaatctcca tatcgcttac ggttgtatat gcggcctgaa
    23281 tattcgtgcc aagaagcatt ttgccatatt gatcaagtgt aacgttggcg atgattggga
    23341 ctttcctgcc tacctttttg attacatcat gacatgcctc tatggcgagt ttgacctcca
    23401 gtatgtcttg gcttgtctct atcaataaag cgtctactgc tcccaagatc aaaccttctg
    23461 cctgcgggag gaatgcttcc tttatctggt cagttgtttt ttgaccaaga tcagggtcat
    23521 cggaactggg caaatagcct gttggaccca tagaacctat gacatacctt tgcctgtctc
    23581 taaattcagg ctcttcacaa acctccctag caaggctggc ggctttttta ttgaactcca
    23641 aggtctcatc tccatatgcg tattccgcta gcttcaaata gttgctgcca aaagtattcg
    23701 tttctatgca gtcagcgcca accttcaaat agcttctatg aatctgcttg atccattccg
    23761 gtttgcttag agttaacccg tcattaaaac catctctgct atctggaaaa tctctaggct
    23821 ctggattcaa gcgctggatt tctgtcccca tcgcgccgtc aaacagcaca attctactag
    23881 ttatagcttc aaggaatgat gtcatatgat aaaatcgatt tcgcatgatc tcagtttaat
    23941 agtttagtcg cgttttggga tgcccatctc gaccctccgg aacctgacgc gatgggtgct
    24001 acatcggttg atatagttat actattttgc tatcttatgc acttgggtgg ccacggcgga
    24061 tgagtcgcaa gagctcatat aaggaccttc cttgattatg caaagggctc atagttcagc
    24121 ttggatagaa tggctgcctg cgaagcagtt ggtcgagggt tcgagtccct ctgagcccgt
    24181 aacggacata gtaatgtgtc caagacaacg agtctttaaa gagattgact cgctgccagt
    24241 tacggacaaa gatctaaaca tattttcttc agggagagca gttcatgaag cagtgcaatc
    24301 gcttttcaat agcaatcaaa gaagattcga gaaggaaaag tatgtcgagt aagggggaag
    24361 catcgatata ttcgacaaaa agaaaaatac atccgtcgaa ttcaaaaccc tcaggtcatc
    24421 taacatagac aaacgcaaac gcttccagca agaacaactg cgatactaca tggcatgcaa
    24481 aattcgccta ttggctacat agtatacagt gtctaatgca cttcggaaat agcccctgga
    24541 ggagatttaa agtgacaatg accgaacagc aacgcttcga atagttggtc atcataagtc
    24601 ttagcaagtt tagaga
//
LOCUS       DAOR01000278            2637 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_40766, whole genome shotgun sequence.
ACCESSION   DAOR01000278 DAOR01000000
VERSION     DAOR01000278.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_40766"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    183..326
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2229..2433
                     /estimated_length=205
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          793 a          368 c          465 g          662 t
ORIGIN      
        1 aaatcatcac ataatccatc attacaaaca gcttgtcaga ctttctaagg aaagtagcga
       61 ggatcgaata aaaactggct aaaaaatagg aagagaacta attatcaatg tcggatggtt
      121 cgaatagaag atttatggca ggcgtactaa tattattatt gccacctttg aggccattat
      181 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnncctt tgaggccatt attctctgag tgaacaatct
      361 tgtcgacatc gacaatcgat gtcctgccga ttcaagcaat aaaacttgat gttagaagcg
      421 atatatcact taaagatgtt ttagcaagat aatacacgaa acgaaaagtt gatgttgtgg
      481 ttaataatgc tggttacgcg ttcgcatgtc ctttcgaaga tttatcaatg gatgaaataa
      541 aagcacagtt tgaaactaat ttctatggat cggtgagagt tatgcaggtt gtattgccta
      601 ccatgataaa ccaaagctat ggaaggatag tgaatgaatc tgctctggga ggaagaatag
      661 cttttccctt tgattcagct tctcatgcga ccacgtttgc tctcgaagga ttatctgaat
      721 ctctccacca tgaagtagag cagtttggaa ttaaaatcat tcccatagaa ccagatgtgg
      781 taaaatccct ctttttcgat aatttaagat ggcaaaacgc acaaagatca tattctccat
      841 atctagatga tgcaaaaaac aaatgcagga ttcgcttctt tattagaaaa tgctactcct
      901 tctaaaaaag tagcgaaaga aatactgaag gcagttacgt ctgaacagcc agaaccgaga
      961 catacagtag gcgatgacgc tgcaagtaca cttgaaacga aaagaacaat gtcaaatgtg
     1021 tagtttggag agttaacgaa gcaatagttt ctacctcaat gatgagagca tgtaaaaatg
     1081 tccaaaaatg aataatgcag cttctttgtc cttatcgaaa ttggctggat gattggaagt
     1141 gatatttgtc catatcatag aagaagaagc aagcccggat ctgttcgatt tttgtaagaa
     1201 ttatgaatga gaacctcaag agctaatata ctgtattgta tgaagaatga aaataaaatg
     1261 aactatgatt tttcaaacag aattgtattg gtaacaggtg gaacaggagc tttgggaaga
     1321 tctataacaa gcgctttcat tgagtctaat gctacagtta tatcgtcata tgttgttgac
     1381 agagaaatag agtcgctaaa aaaagacagt aaaccaatat tacaactagt caaagcagat
     1441 gtacaaaaag aagatgaagt agagaaatta gtttcagata tcctaggaaa atatgggcgt
     1501 attcacatag tggttaacgt ggttggaggt tatcttggag gcaaaagtgt atgggaactt
     1561 gacgaaaaaa tatgggatag gatgatgact ataaatctaa aaacagcctt tctgataagt
     1621 aaacacgttg tccctcaaat gatatcttca aaagatggga aaatagtaca tatatcatcc
     1681 aaaactgcgt tgaagtctag tgggtatgac tctgcttatg cagcatcaaa atctggattg
     1741 ataaggctgg tagagtcgct gtccgaggag acaaaggaat caaatattaa cgtaaattgc
     1801 atcatgccaa acgcaataga tactgaagca aatagaacag caatgccgac tgcagatttt
     1861 acgaaatggg taaagccgga ggatttggct aatgtgatat tattcttatg ttcagatgaa
     1921 gcaaaggtca taacaagtgc tgctatacct acatatggga taggttaaac gaatgttgta
     1981 gctgaaaata caagataata tacgtatatg taaaccaata ttactaaccg cagaaatacc
     2041 cctacagaaa acagattcct gtattgtttg ccgctatgat attctaggaa aagccccaag
     2101 ctaactttca gtgcatcggt atgacgattc tacaagtatc tgacggcatc atgctgaaga
     2161 agtaatttat ggtaaaactt atcgtagtcg tacctcattt cctcctcgtt gagacagaca
     2221 attgaatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaattga atgtattaag tggaaagtac
     2461 tttatgcgtt attgccaata gcaagtcgat tttttattgt tgaacagttc ttttaacctg
     2521 aaaaatttag tgtttactat cttcattttc tacgccctaa tttcatacga attccatatt
     2581 ttggacatag tgtaatatgt gtaatatggg gttttaattc gaccttatga tttgggt
//
LOCUS       DAOR01000279            6161 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35915, whole genome shotgun sequence.
ACCESSION   DAOR01000279 DAOR01000000
VERSION     DAOR01000279.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35915"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3404..3523
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2147 a         1022 c         1135 g         1737 t
ORIGIN      
        1 ctcataagag ttgaagctcc aatgatcgag gcacaaatag ttgaaacata tcttctatct
       61 atgatgaatt ttcaaagcct gatagctacc aaggcatcgc gaatagttac ggcagcaaaa
      121 ggaaagttca ttatagagtt tggttcaagg cgtgcccatg gaccacaagc agccctctta
      181 gcagccagag caagttacat agcgggttgt ataggaactt caaatgctct tgcaggatac
      241 aagttaggaa tacccatttt tggaacaatg gcccactctt ttgtaatgag ttttgaaaaa
      301 gaagaagaag catttaaaca acttaacaaa gtattcccat caggctattt gctggttgat
      361 acctatgata gtatcgctgc tataaaaaag ataattaaat ctggaatcaa tacggatgga
      421 ataagacttg atagtggaga tctgtattcc ttaagtctag aagcaagaag agtgctggat
      481 ggggctcctg ggggcggtta cgctaataca aagatcatgg caagcggaga cctcaatgaa
      541 tatctaattc atgatctcgt taataggggc gctccaatag attcctttgg agttgggact
      601 gagcttagca catcgagaga tgatccagct atgaatggag tttacaaatt agttgcgata
      661 aaagtaccat cctcactatt agctgggagt aatgaaggaa gagttgatga aaaaatattc
      721 tataaactta agagtggtcc tgcaaagaaa acatatcctg gccccaagca aatatacagg
      781 atcttggaaa acggattaat aaaaagtgat tttatagcac tagaaaatga agagaagcta
      841 cagttggatt cacatccctt gctgcaaaaa attcttgata aaggaaacat tctatctaat
      901 atgccgtcgg taaaagagat tcaaagattt cacctacatc aaattaagac ccttcctcca
      961 aagtttcttg atttagaatt tgtacccgaa agctttccag tgttttatag caaggaactg
     1021 gaagccaaga gcagagaatt taaacctcaa taacaacgat aataacaatg ataatgtaat
     1081 agcagataaa gtgccgttcc gattattcaa ttaatctcct tctcttgctg tttgaactat
     1141 cctagtacaa tgatattcga attttggaac acttatcaaa tatgattcag tgtattgaac
     1201 tgcatctaag ttcttattat gaagaatagc cgtattgcaa tcatgaaaaa aatgtgatga
     1261 taagggaagg ctaattgggt tggtgagtag gcctgacatt atgaatatta aaagcgagag
     1321 acaagattag gtgcagcata caagaaaatt tggcaataac taatagtggt cttgaggtgc
     1381 atgcactaca ccaccgactt tatgtatata ttagatatct agattgtatt catattcatt
     1441 catgttggca tacagtttag aacgagaaaa tgatgaaaag gaatactcga ttgcattaaa
     1501 atgccatttt aaatagatat atcaagttaa gatgaaatga aaccgtcatt ggccaaggga
     1561 cataatagta aaaataatgt acctcatgat aattatgaac gaaaaccgag gtatgctatt
     1621 cttgtaaatg atatgttaaa tgatttcata cacggaaagc taaaatgcga tagagctgca
     1681 caaataatac ccaaaataaa actattatta gaaaaagcaa gacaaaagga tatacccata
     1741 ttttattgta atgatgagca ccttcctatc gatacgtatg agcttaaact atggggtccg
     1801 catgcaatga aagattcaga tggagctaaa gtaatagatg aactaagacc atctgctaat
     1861 gactacatag taccaaagag aagatacagc tcctttgatg gaacttcctt aaatagctca
     1921 cttggagaaa cctataatgg aaaaggtgca gataccatta ttatgacagg agttcataca
     1981 cacatttgcg ttaaacatac tgtatatgat gcatttgtaa gaggatatag tgttatagta
     2041 gcagaggatg gagtagaagc tttcacagga gatgatcatg aagctggtct gaagtatatg
     2101 aagcaaaatt atggtgcaag gatagagaag atatcagata ttttgaaaag tatatcttag
     2161 taaagcataa tatgcaatgt ccataaagag aaaattatga gatgaaaaaa tgtactgcct
     2221 gttagataca tatttttgtt tgatagttcg attttactgc tgttatatca atattaggtt
     2281 cttaatctgg aaatgataat tgagagttct tatatttcaa tgcatggcac tgcaacccta
     2341 cttctaaaat acgataattc gcaaatcctc tttatgacaa cctctgaaaa atcgaatata
     2401 agaagaaggg aagaagcaag tccgttggta ccaatgagac gacatatgga caatgttttt
     2461 gaagacttta gaagggagtt gggaagtatg ttccatccgt ggccgtattc gatggcggat
     2521 tggcgttttc cagtattagc tgatgtagaa agagaaataa gactgccact ttgcgacatg
     2581 gttgataaag gagacaaata tgaattacaa ttagaaatcc ctggaattga taaggacaaa
     2641 atagatgtta aagcaacaaa gaattctgtt gaaatttctg gagaacaatc agagaagact
     2701 gaagagaaga agaaggatta tgtctacaac gaaaggtcat acagatcatt tcatcgcaaa
     2761 attccaattc cagaagatat agtaccatca aagattgatg ctaagatgaa taatggaata
     2821 ttgcaaatac atcttccaaa aaagaatcca actaaaccag aagaagaaac tacaaaagtt
     2881 gaaataaagt gacaaggatc aagtcacctc acgactttca cgaagtcaaa attcataatt
     2941 cagaaagtat ctgcttaaaa tgcagtacag agacctcaca aatatgtgaa tggtgtggtg
     3001 cgtgttacaa atgtcataaa ggattagcaa tagaaagaga atattgaatt acaatttgaa
     3061 atgccggata atagagtagt ttaaatgaaa tggactaata atacaaggtc taaaaacatt
     3121 tgaatcttga agctcaaact cctgaattaa aggtagcaga atatatgact cctaatccaa
     3181 ttacggtaca gtctgatatt acttttacag atgctgcaac taagatggcc gggaaaggga
     3241 tcggaaatct aatagttgta gaaaatgaaa gaccagttgg cattcttacg gaaagggaga
     3301 ttctacaata cttatcacaa tggctaagaa ttcctaatcg attgcttgag tatgtcaggg
     3361 ttcaaccatt cgaagcagta aaacctgaca ccactatact cggnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacctga caccactata
     3541 ctcgatgctg ccaaaacaat gatctccaag aagtctaggt tattggtgtt tgataacgaa
     3601 agtaatgggc ttgttggaat aattacagca tcagacatag tcaaagcctt tagaacaaca
     3661 gacataaacc caccattaga acaggtcatg accagaaaga tattcaatct agaatacaat
     3721 aacgctatca tgaaagcagt cagaatgatg catagacgca gaattggaag tgttcttatc
     3781 gtaaataata aaaacaccaa gaaaagcaaa actccatatg gtatattcac agaaagagat
     3841 ctactgacca agatattatc aagagggata gatttagacg aaaaagtagg tgattatagt
     3901 tcgacaccat tgataacagc acagattgga atacgtgcga ttgaggctgc aaatataatg
     3961 tatcttaaca aaatgaaaag gctattactt acagatccac tatcacagga tagtaaccct
     4021 gtatccatag taacggccag ggatttggta gaagcatttc aggagaatta agcaagctca
     4081 cagatacaga ttaattatga ttatcattat tctgtgagtg gagaatggag tatatattag
     4141 acatattcta gcgttaattc caaccatgaa acaaagatga gtaccggtag tattgcagaa
     4201 caaaaaacaa gaaacaccag tattgaatag caactataat actttggatt gatttagtac
     4261 caaaccaacc aaatttctac agaaaacatg gtcgtatata tttcaaattg acaatcgtta
     4321 atagcaatct aagaatctta actatattta agaaaataac caaaccaaag aatgggtgca
     4381 aaaataaaca ttggtaatag catgggacca ggtacttatc gaaaacatct tgttagctgc
     4441 aattattgtt ggctcaattt actttgaaag atggcttgag gaaaaagcac gtcgacgcaa
     4501 agaaacagaa acaaagaaaa aaactatcat cttcgttatt aatgatttag agaacaaact
     4561 gcgatttaaa gaagagtcaa accaatacaa agactacaaa cctttcttca ctgatatgtg
     4621 ggatgctata attttaacag ggaggcattc attactttct ttcgaaacat ttgaagatct
     4681 gcagcatact tattcgtgga tgaaatatta caacacggag ctggaagttg gtaagaatga
     4741 cgaaggtgtt cttaaagatc ttttgaccga tgtggagaaa tctatatgtc aatcacttaa
     4801 gaaactgaaa tgtaacgctg aaaaaccaaa atgtaacgcc acgacatgac tgcaaactcc
     4861 aatagcacgc gtatcatcta ccatgcttgc aattcctttg tatagtcctt agcaagagta
     4921 gccaagtcta gaacttagaa tacttcttat aattctccga tcgctactct aataatatat
     4981 tattattaga tctgggttgc aaatactcgc tctattacgg tcattggcct ataacacttg
     5041 aacaagtatg tagtaacgca gtataagaat tcttttagat tgataatact acctcataat
     5101 tctggctttt gtttgacgaa accgagtaca ccttgccaat tcctcttacg cctattttgg
     5161 cttacgctat aatgctacgt ctccagtctc ctttgttcct atgtcgtagg cctcagcaac
     5221 atcatataga aatatcttgc cgtccgatgc tgaacctgtg cttattactt tcagtatatc
     5281 atcaattatt ggtttagcca tgctatctgg taccagtact tcaatctttg tccaagatac
     5341 gaattctgga acatatctcg ttacacctct accaactgat acaggttcat gctttgaacg
     5401 ccctctaccc ttaatatcac aaaatgtcat acctcctgcc atatgcttat gaagaatttt
     5461 attaacttca attagacgtt catgaggaat tattagatct attctcttca ttgtgtattg
     5521 actctagtag tggtatcgta tgtaataata aacacatata ctacgctttg gttaacaagt
     5581 attgatgtat gaactgaatt atacttcatg ttcttcgtca tacttattgg atataagttt
     5641 ccctcaaact gggaaccgta atactaatgc ttacttcaga aaatagtaaa atttctaacg
     5701 gtgtcttatt tttcttttcc tcttaaaatt gctcgtagta ccaatagctc ttatcactag
     5761 cagagagcag atagaatggt actatatgaa atcactccag atctttaacg cttatgaaga
     5821 atgtccccaa accaatgttg gccaaattgg tcgtatactg ttttacgttc tatcagcagt
     5881 ttgtcaccta gcaaactagg tataagatct tgattattca ttattgctct gaatatgtcc
     5941 ttatccctga ttatgcctac cattctttca ccgtcagtta ttacaaggcg acgaatatta
     6001 ttttgctgca tggtttggat tgcatctcta agggaactat ttattgagat agtaatcacg
     6061 ggtttactca ttatgtcgcg tagtggaatc tgtaataaag atggatttaa cgatcctaac
     6121 agccgtacaa catctctttc tgtgattatg cctattggtt t
//
LOCUS       DAOR01000280            4666 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_53048, whole genome shotgun sequence.
ACCESSION   DAOR01000280 DAOR01000000
VERSION     DAOR01000280.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_53048"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2370..2650
                     /estimated_length=281
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3684..3934
                     /estimated_length=251
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1276 a          761 c          759 g         1338 t
ORIGIN      
        1 tttcagaaag ataacccatt cgagcatttt aaagaatcct tcgttaataa actaacaacc
       61 catgggtatt tgtttacgca cacaattgac tacaaagata gtcttcactc tgttggtgct
      121 actaaattaa tgaactcagt attccaaaat ctagcgcttc aaactatgac tgaatcttta
      181 cttggtcgta taattattgc tatagtttca tttgttatcc tatgggcaat tgtttccatt
      241 cctgtctgga tatcagctaa aatattgacc tcgggtcgtg caagtttcgg acgtgcaatg
      301 ttggtaacgt ctgcaggacc aatactctat gccttggttc tcatcatatc aacaagttta
      361 atatcattcg ttattggaaa tcggtcggca acaataacct cgttgggatt ggttatagca
      421 tttgtagcgt ggatttatgt attcagaaga gggtttgaaa cgggctggat taaggcagta
      481 ggtatagcac tattcgcaat agttgtattt gtcttcatgg gtattgttat tgccttagtt
      541 acacatgtac tcgtacctaa tgtgcctcca atcataacaa cacagtcatt gcaatcacta
      601 taatgtagga atacaagtgt ctaaattttt tcccatgacc gtattcatac ccagcatcta
      661 cttgacagcc gagaacacaa aggggcacga tcaacatggg cgtgcaatcc atgggcttgg
      721 acatggaaac aatcctgaac ggatattatt tctgtgtagc tgaagtgctg ttagcttaca
      781 tcctatcctc agatagaaag gaaatgttga aacaatacaa tcagtaatga cacttgaaat
      841 tttttcccat gactgatact ctgcaatttc ttattctagt taacctcgaa atccagaaca
      901 acttcacgcc taatcgataa cattagcata ttgtctgtgt caacactaat ctgatcaaac
      961 tagttcatgt ttaaatgttg tagaatgagt cccatattat ataatatgaa acacattgaa
     1021 tagttagaga tgaaggatga gaggaaggac tagtagcagg agagagatat gtccagcaga
     1081 taaaccacta ttagttgctg cattatcact tgaataccat tgatatttgc ttaattgatt
     1141 tacatgcggg tgtcgttact tcatttcttg ctagaaattg gtcagcatct ctaaagaaac
     1201 tatagcatag aaatttgagg cttcaaggca ttgcaacatg ttgctcgtta atgtggtcct
     1261 gaagttcctc cagtgaatta aatgacatac cacattcctc acatttatat ggcacattag
     1321 tttttgagga catcaattta tatattgttt ctcacatatt tattaatttc aaaatagtaa
     1381 ctattattat cattttcttg tgatggtttt cataatgggt atgatgttga atggaacgat
     1441 gatcctagaa catttttatg ggtacccaaa gaggtatctt acggagcgaa gtaatttgac
     1501 aatgtatccc gacgcagagg aagagatcaa gatacgtgca ttcaaaaacg tactaagttc
     1561 gttgtacgaa catgtacgga cgtgcaaaat ttgcgatacg taccttaacg ccatattgga
     1621 acacgcagtt aagagtaagg acaagtaggt gcctacactt atatcgtttg gacgagcatt
     1681 ctttccttct cttcttaatg tccggtgtag tatagattgg gaaggttatt atggcttcag
     1741 ctgacagccg cgcgtctata tactataatg tgtttcaaca tgattactta attcatccgc
     1801 agttgtaaat gatgtaccac gcagatgaca tctgtataat cgttgtttga aacaccttat
     1861 aattcctcaa cacacgacaa gaatgttcat agtgacagat tttgaaacaa tggcaagaaa
     1921 tacttgtaaa tttcacaatg tcccaatcac atctgctgga acagtgcaag aactacagat
     1981 tttgtgttga caaaagtaaa acagggttct agcagtagta ttttgggttt aaagacgcac
     2041 aggagattaa gattcatcta attcaataag tcaagatgat gttttagatc ctttaatctc
     2101 gttctaagtg tcatgtcagt tatgtctgct gcattagcaa cctctttttg agttttgatc
     2161 tcacctgtgc ttatgcaaca tgcataaatt accgttgctg cgagtcccat tggatctttg
     2221 ccagctgaaa tctcattttt aataatagta ttcataagat tgattgcttg atgttttgtt
     2281 ttttcactta tttcgacttt atttgccact ctagcaatac attttatagg atcaaccatg
     2341 ggtatcttaa ggtcaagtcc aagtaataan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn ggtatcccca tttctctgca ggcaatatat attgcagcaa ctaatactgc
     2701 agatattgac cttccacgta ccaatctatt ttcaagagcc tttctataaa tataagcggt
     2761 cttctcaatt acggcacccg gtaacccaag tttatcttta agtgtatcaa gttcattgaa
     2821 cgcttgcgtg agatttttat ctgtagaagt atatacttgt gttctagaat cccacgttct
     2881 taatctttga atagtagaaa gcatagatgg attaatttta gtcccgcttg catctttgct
     2941 ggttcttcct atgatagtag atagacccat atcagagcga gctagcgaaa aaggcattcc
     3001 tgttctattt tcgtcgtttt cttctctttc tgctgacgct gtggtaaaac tacgccattc
     3061 cggccctttt tcctctattt tatcggatat tactgctcca caatgcttac aaacaatttc
     3121 gccagagtca ggaatagtaa ctatctgatc agaaccacaa acagaacatt catcatttga
     3181 taatgctctc atttttgttt tagcgtagaa acatcatata cacgttatga caaggcacca
     3241 tttatcgctt gattaagcgc gaaatgagca gaatttgaag caaagagtga tgtagctcta
     3301 aggaagtcta gatcagagag acaaaactgg aaatgccgta accaaagtct gggaatggaa
     3361 ataaaaagct gtgtcgtatt atccagggcg ataacatagg caataaaatt gaaactgtga
     3421 aactatgcga gcttcacaga caagacgcaa aagttaaatt tgactacttc ttcttttgtc
     3481 ttcttttagc atcttctagc gatttgtttt tcttattctt cattttccat ctacttgata
     3541 agggagaaaa cgaagcggta ggttataatc attcacctct catcctatca tacccatcat
     3601 gcatcgaaat tgataggctt ggtgtcattg ctactaacat atttgaacaa atggaaaacc
     3661 aatggttact tatccatcat catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttcatc tgtatcaaat tacataccgc
     3961 gatctgtctg tgtctactat tttttcttaa tagttgatta tgacctttac ggaacctgtt
     4021 gtgatcacat ccattccacc tttcgttcca ataattgctt gacaagatta aaggaaggcg
     4081 tgaaggtagt gcaggctcta tagggaatag gatatgacct tccattagtc tataacaaat
     4141 aagtatcttc aatatacatt ttaataaagt aaattgcaga aagagaggag gggatttacc
     4201 gttagaagaa aatgacccag ttaagcaata taaattctta aaatccatat ctgttacggc
     4261 tgatagtttt ggatttgatt caatttagaa cagataggag cgccaacacc gactggattg
     4321 tcaagttggt aacaagagtc gccgcaaaca aagttgttgt aagaattgca ataacactat
     4381 aggtattgaa ggataataat tctccacaaa ccttttgtta tcgcgccata acaggcggta
     4441 cgtatgtttg aagttgatta aactgcatat acgatgtctc ttaattgacc tgtctaaaat
     4501 ttaaatgcct tgtactgcag aaggacaact aaatcccttc tggtactgag cagtcagcaa
     4561 tcaaaaataa atggaaatgt aatgtctgtg gtaagatatt tgttagtctg tatatattga
     4621 attatcataa actattagaa catagtgaat ctaagagacc accaat
//
LOCUS       DAOR01000281            4205 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_44478, whole genome shotgun sequence.
ACCESSION   DAOR01000281 DAOR01000000
VERSION     DAOR01000281.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_44478"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2255..2452
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3106..3425
                     /estimated_length=320
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1343 a          617 c          723 g         1004 t
ORIGIN      
        1 tatttctgtt ctaaggcgat ccgtaattta cgatgataac gtcgcatatt tttctgttac
       61 agaatctctt ataacaaaga gcgccacaga aaatgtagat cagactgaag gcgaggatct
      121 gtgggttggc tctaccgaag tttctgtggt tgaatctgcc aaaaaacact ttttgtcaga
      181 ttggaaaaat gcgattcccg ccctgcagaa ggttagagaa attgaagaag gaataatgcc
      241 cgttaagact aaaatattgg agaacgaaga tgaaataacc aaagaattaa gacacctaaa
      301 caataatgcc agcagattat ctgtttgttc ggcatttggt ggaatgcaaa tgagtcacaa
      361 gtatcttttt gattcatata tgaacatagt agataggcat caaaaaggag aaggtaaagg
      421 aatgagatgg attatcaata tagataaaga aaatctaaat ttagtcaaag tatttcttaa
      481 agcaggaata cagataagac atgtaaaaaa tatgccacca atgaattttg gcgtctcaga
      541 taaagaatta gcagccacga tagaaaagat ggagggtggt aagatgagtc agagtttcct
      601 aatcagtaat gagcctctct atatcaacca ctttaattct ttgtttgacg agatatggaa
      661 aaatggaatt gatgctgaag atagaatacg agatattgag gtaggtgcgg agtgggcaga
      721 tgtagaagtt attccagtgt catctagagc aaaggatcta tacttaaatc ttgtaaaaaa
      781 ggctgaaaag gaaataatca taatgtttcc tactactgat gccttcatta gacaaaagaa
      841 tatgggagta atactatttt cagaagaagc agcaagagag cgtaatgtaa aagttagaat
      901 attgatgcct tcacataaat caaatgaaga tatagtacga cacttgaaag aaaattatgc
      961 tgaatgtatt aatattagat atattgaaaa aatgtcagat actaaagcaa cgatcctggt
     1021 agttgacaga aaagtctcat tggttatgga attaagagat gattcaaagc gaactttcga
     1081 tgaagcaata ggattatcaa cttactctaa tagcagacca ggcgttttat catacgtatc
     1141 aatatttgag aacttatgga aacaaactga attgtatgaa cgattgaaaa atcaagatat
     1201 aatgcagcag gaatttatta atatagcagc tcatgaatta cgcactccta tacaaccaat
     1261 aatcagttta acagaaatgc ttcgatctca aataaaggat gtcaaacaac aggaaatgtt
     1321 agaagtcact attaggaatg caaaaagatt acagaggctt acaaatgata ttctggatgt
     1381 tacaaaaatt gaaggcagat cactagaact aaacaaagaa gactttaatt taaatgacgt
     1441 agtgataaac gctatgcatg atataacatt aggcagagac ttccttaaga acgaaaacat
     1501 aaggctgtcc tacaatcctc atagggatat tttaatacaa gcagataaag gaagaatatc
     1561 gcaggtagtt tcaaatctct taactaatgc tgtcaaattc actgcagaag gaactatact
     1621 aattagtgtc gaaaaagaca agagtagtaa taacaacaac aataaagcaa ttatcgttag
     1681 tgtaaaggac tctggtcaag gaatagactc gagtatatta ccacgattat tcacaaaatt
     1741 tgcatcaaaa tcttataaag gaactggatt aggcttgttc atatcgaagg gtatcataaa
     1801 tgctcacggt ggtaaaatct ggggcgaaaa taacccggat ggcaggggag ccacattttc
     1861 attcagtctg cctctagagg aaaaccaata gattcgacta taggtttacg aagggtaaga
     1921 aatactgcat acgatgtgaa gtttattatt gctatgatgg tgtgttctgt cctgttgcgg
     1981 tatggcatta cgaaggtcac caactaataa aagagataag gaaaggctga gggcaattcc
     2041 aacttagaag agaggaagaa caagatagaa taatcaggat tataaaagga ataaagaagt
     2101 atcctgttga tcaaggcaag ccaaaataaa atactcacaa ccacagatca aatccttgca
     2161 attactacta ctactactac ttaaaaggga ttacgactga tacacttgga aatgcttggt
     2221 tttatcattc gacattcaac agtagtacaa tagtnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctataat
     2461 aaatctggca ggaggtcaac tcatctttga taacgacaat aatgtggttt ggtttactga
     2521 tgccagaact aactcaattg gcaaattaga cgtcaaaagt ggggacatcc aacttgtcag
     2581 tgtaccaacc ccgaaggcag gacctatggg aattgtccta tctccagatg gtaaaagtgt
     2641 atggtttgca gaaattactg gaaataggat agcaaacctt gatattcaat caaacaaaat
     2701 tatagaatat ccagttattg gtagctttag tagtagcaat aataatggaa ttctgaataa
     2761 tcaagacaat ggacctacgt tccttacttt tgacagcaaa ggcgtgctct gggtgaccct
     2821 ctcatattca agtagcgtat tgcgtgttga gccttgggca cttgttccta atagcagcac
     2881 aatgggaatg tccccattta cacttgcaaa atcagaaaca ttctctccgt ttggtatcgc
     2941 agtggtagat tataaagcag gttcacaaca aagaatgtat gtatcagatc atggatcaag
     3001 tagaataatc tcaacagatg ctgggcccaa tctcttatta caatcctatt tgtcatattg
     3061 gacatctcct tcccaggtat atccgacaac tctgccaggt caaatnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnntgttg attcagggca aatgactgaa tacgatattc ctactggacc actttcaacc
     3481 gcactcttta ctgcaatatc tggagatgga aaaaaggtat ggtttacaga atggacatca
     3541 aacaaaatag cataccttga tacgacgatt ccagttccac tcactataca agtaccagga
     3601 cattataata tcaacaataa taatagcaac actaatacgc tgttagtact taaaagaaac
     3661 gaacctaaga tactagatgt tgcactgcag acagataata ataacaaaac tgctcttgag
     3721 gctagttctc cttctacgtc ccgatcttta ttgcttaatg aagttggatt atcatttgta
     3781 ggaatgggtg aatctggaac tgttgggata aattatacag cacagcctca aagaattgac
     3841 atggagaaaa attcaacagc caattcacag atcgcgcttc aggtagaaaa tgatgaaaac
     3901 aatagccgta tgataagccc aggtcaatat actattatgg tgagggcaac tgctctcgaa
     3961 aataaagata ataattcact tgtatctttg ctctatcctg ttcagattat gctggatata
     4021 ccagccccac caacaaatca acagcagcca caacagactc accctaatag tattagcaac
     4081 aactctcaag taccagttaa tggatttttt gactatacat cgttaggcgg catattacgg
     4141 atttttccat tactagtagc aattggttta attgcgtata tagtgtatag aagaatagaa
     4201 agatc
//
LOCUS       DAOR01000282            3938 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42618, whole genome shotgun sequence.
ACCESSION   DAOR01000282 DAOR01000000
VERSION     DAOR01000282.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42618"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1307 a          736 c          850 g         1045 t
ORIGIN      
        1 aaggtcagct gttatgtttt gctgataatg gtacgccaaa cctaatcaaa catctggttc
       61 acaatttcac agggatacaa accaattcag gtattccaac actgccagca ttcccgcatt
      121 aagccatagt aataagagat gcgccaaaat aaagcaataa tttcattgat tgaatttcag
      181 agatagtatc ggcgggctcc gacagatttc cattctagct ggctggatta gccgccgaca
      241 tgcatccaac aaccaccgat gtgctgattt ctcgacacac gtgtttacag cagatctcat
      301 cggctacgaa taaagcggga gattgactgc aagacatgca cccaaaagaa tgtgaaaggt
      361 ctttgtttcg catgggcaca atcgccttta ttcttggtat tgtcattttc ctcgtttcaa
      421 ccatatttcg tccaagcagg gaagatccta caaatcatct tcgtgtgttt gccgaatacg
      481 cccagagtaa tatctggatt gcagctcaca taggtcaact tgcaggagga ttgctggtct
      541 ttggaggagg atttgttgct ctataccgct ttttgggtca aacatcagaa tcaggtcttg
      601 tttcagcact ggcgtggtta ggtcttgccg caacaatact tacagctggc accttggtga
      661 tactacaagc tgtagatgga atctcgctaa agagggcggt ggattcatgg gccactgctc
      721 cccaggcgga aaagatagcg gcattccgtg tagctaaggt ggatagaaat aggtacccct
      781 aacatgtttc gcatactcca aggagcgact ggaatagtct ttggcgtcgc tattgcaatc
      841 aagcgcggtg cttttccgag atgggtagga ggaatcggaa tatttgcagg catattagcc
      901 atgaacgatg gtatatcagt agccttcact ggttttgtag gtagccatct cgccgcgact
      961 tatgagttga cttacgctgt atggacagta attgtgggga tctatatgtg gaagagaaac
     1021 atgacacaga cggttacacc ataaacctta gcggcccccg ctgcgttgtt gcacatccat
     1081 taacaaattc tcgacaaaga gccatacaca cactttatct tgtattcagc aacatagagt
     1141 gaatgaaatt tggggccata ataaaggatg agctgatcct actccctcag gcgagacaag
     1201 tcctagctaa aatcacaaac gggtattctg taaaaattat aagtgtattc ctacacagtg
     1261 taacgtgatg aagaaagact gtattatctc aaaaattgaa gcctctcaag acggaagccc
     1321 ctacgtgtat gtcacatttt ctgatccaaa tgattacaag ccaggcgccg aaaaaccgca
     1381 aaatcctctt ggtgcgaaca tgatggcatt tacttcccct gaggacatga tgaagaattt
     1441 gccaaaagcc atgtcaaata tttccagggc cataggtgga gccggcccac cgacggattc
     1501 ccctacgttt aaaatcagta tgagggagta cgaggatatg gccattagag tgggcgacaa
     1561 gatcaccata gaaattaaga aatcagacag cagcagcggg agatagatat tagtatagtg
     1621 aaattccctc gaattcgtat tcaaaggaag aaataaacca attatcaaac gcctatgaaa
     1681 acaaaatagc acgctattct attgaatcac ttgcatattt agtcatgggc ccaagggggt
     1741 ttggcaagac tttaaagtgt aataaaaaat tcacgaggaa atctaatcaa tagggcttga
     1801 acccataagc tattgcttgg agcgcgataa cgggttcacc gtcttcttaa ccaacttcga
     1861 ttattataga tttgcttgcg aatttattag cgcttcagag tgtctaatgt ctgaagcgtg
     1921 aagttaaatc taatcttctt atgtgttcta gcgccgaatt tgacaacttc tataatacag
     1981 catataacat tcattacagt ggttatatat cgctatgtaa tgcagaataa gggaacctta
     2041 ataagtgcag taaatagcgc aaatttccag tttgtttatg tagcaatctt atcttttagt
     2101 tatgtgtcgg tagcatgaat tatatatatt tcatgtaacc aaactatccc gtggagatag
     2161 gatggtgaca caaaatatac aacttggtcc taaggaaacg aagcttttct tcacccttga
     2221 agaggaggga agaagcatct ttactgcaaa tgatgcaaag agaatcctcg gctcaggacc
     2281 tgcatactgg ttggtaatta gcggtctaaa gaaaaaagga agaattaggg agatccagaa
     2341 aggaaagtat ctcttcatac ctgcaagagc aggaatagaa ggctactggg ccgaagaagc
     2401 ctatgtaatc attccgtatc tgattgaaga gtattatatt ggattctgga ctgccatgaa
     2461 cttctggggt atgacagaac aaattcctca taccgttttt gtcgtgacac cgaaaagaaa
     2521 aaagagacga gttttagaat ttggaaacca aagatacgag tttgttactt tatcaaggaa
     2581 aaaattcttt ggattcgttg aagaaaaaac tgctaaaaca aagttcaaca tatcaagcaa
     2641 agaaaaaaca atagttgacg gattaatgcg cccacaatac tgtggtggga ttcatgaagt
     2701 aactaaggta atgtggaacg caagaaaaga ggtgaattgg aagacggttt tagaaatggc
     2761 agaaagaatt ggggttaata ttgttttaaa aagactggga tatatgttag aaactcttga
     2821 aatagaaccc gatatttcaa aattaataat gaaacaaata agacaatatc cgtatcagta
     2881 cctagatcca acagccatca aaaagaaaat tgagtactct aacagttacg gattgataat
     2941 caatataaca aaaaatgaat tactaggttg gagagaccac taatggatgg gaaagagctc
     3001 agaaagctag caggcaaaca taaaataaac ttgggaacac tggagaaaga ctacgcaatt
     3061 accaacttgc tctccatcat agcacgtttt ccaaaattgg attcaatggt gttcaagggt
     3121 ggcacagcca tgaaaaaaat ttattttgag ctattcagat tttcagaaga ccttgatttt
     3181 acttgttcag aggatgtttc tgaaggattt gcaaatttca tagaaaaaga aatgaaaacg
     3241 cttgatgtga gatttacaga aatagcagaa aaagaaggaa aggatgaaag cttcaggttt
     3301 aaagctaaat atgctcagtt taacggagca actgccagtg taagattgga tttgagcttg
     3361 cgtgatgatg tactcactga tcccgtaacc aagccagttc tacatttcta caataatctt
     3421 ccaaattcct tcgaggtacc taccatgtct ttggaagaga tcatggcaga gaagataagg
     3481 gccataatct acgctaagca tccaagacac cttcatgacg tttggtatct taacgaacag
     3541 aaagtaagcc tcaaccctga tatggttcgt acaaagataa aaacagttta cgatgatgaa
     3601 tttgatctgg ataaattcag agatagtata gcagaaaaga agaaaaactg gcaaacagat
     3661 ttggggcctc ttttgtcaag cgaccctcca ccatttgata ttgtttcaaa aagggttctt
     3721 gagactgtta ccaacgcaat gaaataacag accttacaaa aggccaagtc caaaaatatt
     3781 cataactgtt taaaatgatc tttcattatt cggcttgctg tacaagcgta gagggtatta
     3841 ccaattatga ctgtagaaat agaggaaatt cgaaaacgtt gggtaagatt gcggctctgt
     3901 aacgtatgca gcaaaagctt tcaaagcaac agggaaat
//
LOCUS       DAOR01000283            6471 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_30505, whole genome shotgun sequence.
ACCESSION   DAOR01000283 DAOR01000000
VERSION     DAOR01000283.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_30505"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    647..844
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2020 a         1119 c         1167 g         1967 t
ORIGIN      
        1 gaattaggtt gaattttctt tctatttcta tagcatatac acttcctagc agaaatgtaa
       61 cccaagtctg taagtgaatt tgttacctat gtgtgtaagt acaacaacta actaatatga
      121 taaatcaaga atgctactta ctaaagtaga ataaggaaga tctgtatctt acagatacat
      181 gggtgccaca tttcctcaaa tttcacgatt tcttcccaat aatatggaaa tacattcatc
      241 agctgatatt gtatcagacg cagtagatac tatttctaca atacaagaca tactgtcagc
      301 aatctcagac gataaatctc tggcaatttt tagagtgata gctgaagagt gcaatactgg
      361 agtggagagt cttatcattt gtagaaacct acaacttact cataagaagt attatcgaag
      421 attagcagct ctgatccgat atagtttgat aataaggaaa gatgggagta aaaaatacat
      481 actcacttgc cttggcaaag tggtttatac tactctatta tcgattcaat acgcgctcga
      541 taatatttgg aaattcaggg caatcgatga aatgaaaatt tgtaagggtg tatacggaga
      601 cattgacaag attgtaagca gcttagtaga cacattagtt cataaannnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnagtaga cacattagtt cataaagagc aaatcaagga aattcttaag aaatgaatcg
      901 gcagggagaa agcaaatgag cggagaaaaa gatctagatg cgctttccta tgtcattaaa
      961 aaagtcagaa gggcagtatt gagaaattcc gttgatggcg aattaggaaa gatgcaagct
     1021 gagtgttatg gacttctagc cgatttacag gatgaagttg cgaggttgta tcagtaatta
     1081 tagcacgatt gcagttgtaa cgttactgac ttcttcatgt tagcgaaaac atctccgagt
     1141 atcatggtac gtagtcaggt ctcgtttggt tatagcatca acatcaaagg attttaaggc
     1201 aagtactagg ttttttgaag gcactatgtt cagttgatgt aacctattac atagtattaa
     1261 aaactaacag ttggtatatg gaattacaaa aatgagttat aaagtgagag ttttaagcga
     1321 atctataaaa ttttatcttc ctcgaacaga aggatattta gaaataatac gtcgtatagc
     1381 tcagcaatat gctgccatgt ctctcataga atttgatggc tattttgaag gtaaatttga
     1441 acccactaaa tatatgcggg tagaagtgca tgttaatgat gttaaagaag acagtttaat
     1501 agagtttgca aataagattc gcattatgct taaacaaaga agtcttgctt ttgaggtcaa
     1561 taatcgtcta atacttgtag aagaggctta gaccatactg caagtttgtg cttcttagct
     1621 acaagtttat tgcaaggagg agagattgta aacagatggt aagtgtggac gaaggcgaag
     1681 atctagagga aaagaaattg ctaggctctg gtggttatgt ttttgccaaa ctaacagagc
     1741 aagaagaact cagtgccaat cttggagtgc ctcaaatatt tctagctaat gtaggaagat
     1801 taaatgaaag cagattttca aagtattatt gcaataaatg tggaaaggaa tatcctggct
     1861 ccgcagtaat caattacgaa aatccaaacg aagaactagg ggaaggtgtc attcttgtcg
     1921 aaaaagggga atacaagtgc agaatatgca ccaacgtaat agcgcattac cgtaagttca
     1981 ataaaaggaa ataatggctt agatttgtag aagttagcat ctaatactaa cttgtattca
     2041 tgactaatct tctcatgata taatgacaaa gacttataca actacaccat caatcactac
     2101 gaccccttct tatggcaatt ataacaatga tgatatttta tttgagagaa agatagaaaa
     2161 tataacagaa ggactgactc tagagtattt caagtatctt tataataagg tatcaaaagg
     2221 taacgcattg gcgatagtaa attatattac atccatgaaa acggagatta atttatcgga
     2281 tcattacaaa agagacatta tcaaactatt tagcatattt tcaatatttt ttaacaatca
     2341 aagatcattc aaggacttta ctagagaaga catattatca tttctggata agttccgtaa
     2401 agccgaaagt cttgacccat tgcataaatg gataggtaca tataacaagt atcggatgca
     2461 cttatcgcgg ttcttcaagt ggttgtatta tcctgatatc gaaccagata caccactgct
     2521 gtgaatgacc agcgcaggca tcaccgatgt cgtatatccc gaccgcgcca tcagctttac
     2581 cggctacaac tccatcgtgg tatccgacat cgtaacagga gggccatcct gattgatcgc
     2641 agtgttgtgg atctgccaac ataatatgaa ccgaccatgc accgatcatc gctaaagttg
     2701 ctaatgaaac tagcacgatt agaatagcat tcgacttgat ggcccacatt taatatcgtc
     2761 tgtatctaca ttagatttat gcagtagaat cttgtattca atttgaaaca tgagtgtcta
     2821 tctaaattcc aaattatcct caagtacatt tccaggtcca gggaccattc aggataacta
     2881 gattggaggc acgctgacaa atcatcctga ctacaaagca aagtctctaa gtcttttctg
     2941 tcttactaca cagattgttg gtccttgatg catgacaagt atacatagga tatctgaaac
     3001 atgaatactt gaatcaccaa gcctccgcct ttcctcatgc ctttgacaat gcttttcatg
     3061 tttgattgac ggtataaccg gatttcaaca ctgtcgattg accagttcta cggaatttat
     3121 gatcgctgtt tttcttgttc ctgtacctgt atcgtccatt catttctgct cttttgttgt
     3181 tgtattatct tactcgtccc aactaaaata tcttgaggat atttcatctg tgatatatga
     3241 aaagtatttt ctatgggtct tagttttctg actcaccgtt tattattggt tacttgaggt
     3301 acaccatcat gttcataggt cagcagtggt ggaagttgtg ccagtatcca caaatccgaa
     3361 caacatggaa gtaatcaaaa ctaaagataa tactgctacg atagcggccg tacccactcg
     3421 cttttacata tcgttaaacg ccattcagtc ataataagga taaatgtcaa ctatagactg
     3481 atgctttaac tgccccacat caggccaaaa actgtttata ggagaagacg ctctatacca
     3541 tctcagaata tttaaagcgg attatcgcaa ttcgagtaag gttaaaaatc tactacacgg
     3601 tagttattat attgtcgtct tcggaaaatg ccaccacgtt catcatcaag ggaaggaaac
     3661 gagcattaaa agaagcctac gacagatcgc ttaatgtcaa acattcctat tattgtatta
     3721 ctggcgatga gttagtaaaa agctacaagc catttatacc cttattaaag gatcaggtca
     3781 gccgtggcgg tacttcatat ataatcgcga atataggaaa ggaaacctta attgcagtta
     3841 aagttctcac tgaagctggt gcagaaatta gacatcttga tatttctgtt ctaaggcgat
     3901 ccgtgattta ctttaatcag cacgttgcaa tcttcttttg cagcttctac ctatgcaatc
     3961 tccatgtaga tgtaaatttg cctatattat ggcaaattta gaaacatcta ttctaggttg
     4021 tggatatttt aatttcattc tatctaatgt attgacgatg atttgagcga cagcccaatt
     4081 acgaaaccac tttaaatttg cggggataac ataccatggt gcccagtggg tactacacct
     4141 gctcagagct tgttcataag attccatata cttatcccaa tatttcctga ccagaagatc
     4201 accttctgat atcttccagc gttttgttgg atccattaat ctctcttcga ttcttttttt
     4261 ctgctcatct ttgctaatat gcaaaaagaa ttttagtatt gtcacatgat tctctgatag
     4321 gtatctttca aaatcattga tttgtccata tcttggagtc aactcattct tcggaactaa
     4381 attacgaaca cgaacttcaa tgacatcttc gtaatgagag cgattaaaaa ttgctatttg
     4441 accttttgga ggacctgcca tgtgaacacg ccagagataa tcatgcgaaa gatcttcttc
     4501 agttggaact ttaaatgact ttacatagca actttgaggg tttaatgctc ccattacgtt
     4561 acgaattgtt ccatccttcc ctgaagcatc aactccttgc agtataatca caagagagtg
     4621 gctattggct gcaaagagct tgtgttgtaa ttcggacatt cgactagaaa aatgagccaa
     4681 ctccctctca acatcttgtt ttcttttgtc tgcatcatag tcaggatccc aatcttttaa
     4741 cttcaacttt cttgttgttt ttcctggctt tacaagcaat ttctgattca atcccattgg
     4801 taatcttaat catcactatc ttggatttaa attgcaagat atctcttgct agatattggc
     4861 agtctaaacc ggcatggtga tatcttcaga ttgaatagat ataactagaa ttacgtgctc
     4921 atttataata taggccagat ttgcctacac cacttgattt tatcactatt gatattgttg
     4981 tcaccagaat tccttaactt ctaaacaacc tgacagcctt tatcgataca ttgactgaca
     5041 ggagaaatat tccccaagca attaaggagc ttatcataga agcctgatat ggttgtgccc
     5101 ctagctgaag gagttgatag tggatagaaa ctttgatcag tgagtatatt atttcagaaa
     5161 cggaaaatgc agcaaataat ttccgtatat ctcctctgat cctcttcgaa tccctctttc
     5221 ctgtttgagg gtcattatat tttagtctat tatctctata aaataggaag gcaaagagcg
     5281 gaatgtaaac tccatattcg gttgctagtg caattataga atttgagagg ttatttttat
     5341 caaattgcaa atatagttga gtaactagag ccgcagtaaa gaaggcacaa gtcccagaaa
     5401 aaattaaatt cttgttaaaa agtatgacat ctctatattt taagtaaaag ctgttaatac
     5461 tgttacgctc cttagcgccg ttattcatat ttgttagaag ctattattgc agttaaaggt
     5521 agggatgcaa catggcatct tcgaagagac caagtattaa agatgaagaa tttagaattt
     5581 atacttggtc aagaagtagt atatgaaaca tttcggaaca taaagcaaca aattctgtag
     5641 aactgcggac aattgccatg actgggatca attagttctt gtaaactcgt tgcaaacatc
     5701 acgaattagt tcttgtaact tatctgagtt cgtgaatatg tgtggtaact ctctaattct
     5761 aaagcactaa aagataaccg acaaatttta aacacagtga cctagatgga attaagcgtg
     5821 ttagcttctg ctagcttggc tataaagtaa gaacatgatg agaataatga aactgatcac
     5881 ataacctata gatacggaaa tatttatttg gtaggtctag aatcaattga acattcatca
     5941 gctcttgcag ctttaacatt tgtagatatt agtggcgctt tcgtagatgc agtaatcggt
     6001 atgggattat ttgttacatt ggcaatcaag agcagacata tctttgggag gatgccacct
     6061 agttcatcaa tgcacaataa agaacgtgta tttgctggtg catcattttg ttccgaattt
     6121 tctagggctc aaataagtca tattgcatac atcttacatg gatatcttgt tctaaaatca
     6181 cgttagcagg atcgacagat tcgtgaatct aataacttag ataatttaat atgataatga
     6241 tttagggctc ttcctaacat ttctgttcgt tgtgttcctt cttcgcgttc tgtatgagcg
     6301 taattctttt gattttcctc cgattttaat tccatatact tcagctagtt tctctacact
     6361 aagttgatac gaaaaaaaag actgcaagat gatggaacat cggtcagaaa gcaggaagag
     6421 aatgaattag ccatgttgct gtctcttata cacatctccg agcccacgag a
//
LOCUS       DAOR01000284            7851 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_9565, whole genome shotgun sequence.
ACCESSION   DAOR01000284 DAOR01000000
VERSION     DAOR01000284.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_9565"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3365..3589
                     /estimated_length=225
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2469 a         1442 c         1430 g         2285 t
ORIGIN      
        1 ctatgtaagt actacaccta gtaaagcacc agcgagaatt accgatgatg ttgttccatc
       61 acccacttca ttatctacag acttggcaac ttcaaccatc atttttgccg cagggtgctc
      121 aacatccatt tcttttagaa ttgttgcacc atcatttgta attgtagcat ctccaataga
      181 actaactagc atcttatcca taccacgtgg ccccaggcaa gttttcacca tatcagctat
      241 taccttggct gcggcaatgt tgtttctctg tgcttctcta cccttatttt ctttagttcc
      301 ttctcttaaa atcacgattg gcattccgcc agccgtattt tgaaatgtag ccatattcat
      361 tatcacctaa tagaatatta ccactgcagt atagcaaaag ataaaaagtt ttccaaaatt
      421 tggccgcgta gactatttag tgggatttta aatcagcctg ctgcacagcc atcagatttc
      481 aagaccctta attccgctaa gaagttggga tcggtagcag ctaccgtgct ggtgctatcc
      541 taaaaatgtt cattacaaca ttcattgggg agtgtccata gcatgcatca ctagattgtt
      601 attgttgccg ttattgttat tattgcccga ggtccctgct ccagcattcc atccatcaca
      661 aaagtttgag ctgtgaccac tcggacagct agttccggga ttagcctgtc cgtctgaata
      721 acctatgcta tagcaggaaa gacttcctga cttatcacaa tgttgtgaat ctgcaagcgc
      781 ctcaccacgc gcactgatag tcatcattac tgctattgcc gctataccta caaaggcgct
      841 gaccatcttt gtagctggac ttaccaccga cactataata tcatcgatac gctttttcta
      901 agttctagta tcctacttat attatacagt aaagtcagta ataggtcttt gaatcaatag
      961 gtacgcatca ttgtactgaa tagcgttacc gtacattggt aataggatac aaacaaagtt
     1021 tatttttgca tatcacaaga tacctcgcag tatcttccta aaaagtagcg aaaatgagtg
     1081 cgtctacgtc taacctcgta aaggtttagt atttaagtta gcatcgtgat cctacaggat
     1141 ggtctgaatg gattttaccc agaggaaaat catagtaagg gataacataa acgacaacgc
     1201 aaaacgaatg aaggattctg ttgaaccatt tcctcactgt tcaacgaaaa gtgaaatgat
     1261 gacacataga aaacacaacg aaggatctct tgaaaaagtt agatgatctt actatcttat
     1321 aattttctta acaatctatg aagaactggc tacgttgcat acgacacctt tttagttcac
     1381 ccaacaaaat catgaagaat taaccataaa taatccgtag ctccacgcat taggtatcaa
     1441 ggatctgtca ccttttcggc aaatacatgt ccattgatat gctcatacaa cgccaccagt
     1501 tggagaatag cttgccctct gaagcatggg aacagaaact tcgctcttct ttatagagct
     1561 agaaaatatt tgatactgta tcctttagtt ctattgctac tttcttacct ttttcatctg
     1621 caatgatcta ctagatcttg aaccattact tctaccacac cgtctaaagc tgcacggaca
     1681 acagaaacct gttgtattat atcagaatgg tttcgatctg cagctatcat ttttcgaata
     1741 gctttgacat gaccctccat cctggccaat ctaatttttg cgtcgtgcta tgctatttgc
     1801 gtataggaga ataaggtcct gtagacatag acattcttcg ttagtcaacc ttaagggatt
     1861 gtcttctgac ataggtagcc taggactagg cttctctact tcaatgatat tatttgctac
     1921 tgccttcaac attgaagtat ccgttttcct tctaccaatt gataggccat acttagtgac
     1981 atatactttg taggactatt caaaatcctg tgcttctgtc gctacaactg ttggtaatat
     2041 cttgaatgtc tctgggtctt ctttttctaa tctggaccag gaagcacaaa attccattcc
     2101 tggatatttg ccttgtttcc ataaaggagt tgattgagtc atttggttac tcattgtgac
     2161 accctcctgg ttagtaaagc acaatctctg caccttagaa accatttgtc ccagagttgt
     2221 agtttgcccg atcatctttc gcatttttta ttcgttaatt gttcagagtg agcgttagaa
     2281 ttggaacaag ctggatatcc cacagcctgg gcaagatttg agcgtccacc cagattgcag
     2341 actgtaacaa cacccaatca aaggagttgc aacaatgtac aagacactaa atcatataaa
     2401 taataatcat atagtcgttg agcgcaaagg caacgtcaaa gtcaagtcta ggacaaacaa
     2461 aaatgactaa tgctcctaga ggtaagaggt atagtcttcg tagtggtttg tattttgcaa
     2521 tggtagatat ctcaagaaaa tatgagatat cgactagaga ggtcgagata actttgattc
     2581 gtacaatgct gaaagaaaaa catggatttg tttgtaatca tgaaactgat aagattagcg
     2641 ttagtcatac taaaaaagtg catattgtag ctggtgctgg acacgaatgg aaaagttgta
     2701 tctagtagga gtagttcatt tgctccccag aagacaacct ttgtaccaaa gaaagacttt
     2761 atagaaatgg agaaggagca agacaatcat ttgaaaggta ttcagttcga taaacgtggc
     2821 agcaaataag taatttgttt acttaatttt ttaccttcaa atctacccct catataatta
     2881 gattattgga ttattatata attgcagtaa aacacgcaat ataaacaaac taaccatccc
     2941 tttcctggag ggtaaggtgg tgctaactag caatcaaaac tggataattt ctcgtatttt
     3001 tagacatcaa ggagtctata tcctctcatc atacgtcgta ctaagattat tctttttgtt
     3061 tccgctttaa ttttgtctta gagttgccgc aaaaaaacta tacaattcaa atgaaaaggc
     3121 atatagacct catattctaa ggagaaaatg gtcttcgtat tgagtaagga ttttgttacg
     3181 gttgctgata cgaaggatat ccctgcaaca attattacgc cgatactcct gctaggcacc
     3241 ctttattttt tactaaataa tatgagaaaa tttttacctg ttgctccatc tctcttgata
     3301 ttagtctctc tgatgcctta tgctaacgca caaaccaatt tgactggcag ccaaatgaat
     3361 atgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gccaaatgaa
     3601 tatgactggc agctagttaa agttggatca tctactttta gcatttatcg tttggcgtat
     3661 ctaatcttat ggttttgagt ttactagaat ctaatcttca acgattattc tatgaagata
     3721 ttgtaaaaaa ggtaatcaac atttgtcatg aaagtgatgt ctatgttact acatggggtt
     3781 ttattgagag taatagtaca agcctctaaa gcagttgata aataccttga agaatgcaaa
     3841 actttggtga gattattgta atatataccg tatgatatct gcaaaatagt tgctattttt
     3901 ctctccccag atgccaacaa aaaaggtaaa cctaaacata atttaagggc agagatttca
     3961 tacctaggtg atccatgaaa ttaatcaaat caaggatatt gttcgatggt gtcgatgaaa
     4021 aagagaatgt gctaatagga tttgagggcg atgaaataag gtatgttggg agcagaaaac
     4081 ctgaagacac agatagtgag ataattcaag aaggcgatga tatagttgtc actccagcat
     4141 tcatagattc acatagtcat attgggatgg taagatcagg tgagccgggg aaagaggagg
     4201 aatctaacga acaaatgaat tcggtgtatc ctttggtaaa tgcattgcac agtatttaca
     4261 tggatgatcc atcctttact gaatcagtcg agagtggtgt tctctattct acagtactac
     4321 caggcagcgg aaatatcatc ggaggaaaag cagttctgat acgtaatttt gttcaggata
     4381 ttggacaggc atatatcatg gatgttggca tcaaagcggc attaggatat aatccgcgta
     4441 gtaccatgga gtggaagggc aataggccgt caacacggat gggtgctgta gcaatgctgc
     4501 gtgagaactt cattaaagcg agaaaaatgc aagcacttct gcaaaaagaa aagaaagtca
     4561 tagatgaagt agagccaatt acagaggtat tcatggatat actctcaggc aggttcaaaa
     4621 tgatgttcca tgttcataaa gaagatgaca tcatggtact aattcaactt gtaaaagaat
     4681 ttggaataaa agctattgca aatcactgct gtgatgtaca cagggaagaa gtttttgtag
     4741 ccttaaaagc tgcctcaata ccaattatct acggtcctat ggatgcattt ccatacaagg
     4801 tagaattgaa gcatgaaagt tggagaaacg tagaaaaatt attgaattcc ggtgcaaaat
     4861 tctctgttat gagcgaccat cctgttattc tacaaagaaa tatcctttac agcttaaggc
     4921 atatgctacg attcggttta tctaaagcta atgcaatatc taaaatcact aaagatgctg
     4981 cagaaattat aggtcttaaa aatataggac agatcaaacc aggctttaaa gcatcactag
     5041 ttgtttggaa tggagatcca ttttcattgt ctagtcagcc tatattagtt cttgcagagg
     5101 gaagaacagt ctatgatcaa cgataaagag ataggaataa cgtacgagga agctactgcc
     5161 tgaaggataa gtgctgtaac agcacgagat ttgtcctgat gctgcgacgg cctatagcgt
     5221 cagaatccta caccgcgatt cgtgcgttct atcattaagt gcgtgaacag cctgtcctat
     5281 tgtactcgtc aatttatcga gcgcatacga tttcggattt attaatcctt tattaagata
     5341 aatatagaag tttcgcacct gctaatcctc tattagatga aatttctcga aaaggccatt
     5401 tacgatcgaa atacgcctgg cattcttatg agctttgttg gaaaatggtt taccggagta
     5461 tgaagaaaag aagctcttct ctattatgtt gtataatatc ctcgaataag tggtaatttg
     5521 atttcttttt tttattgcag acattgtttg gaggaaaacc tgtcataatg gcattagcag
     5581 aatgacaagc aaggctgctc taactaatat aaaaccattt tgatagatcc cttaaataca
     5641 ctactgtcaa taaacaaaaa ggtggcattc tattgccaag aaccaatgga agtcgcaaga
     5701 aaaaggaaat tgctaagaag ataaagaagt ggttagctga ggaagggttt caagcaagta
     5761 aagttcagga cacaaacacg gatttccatt ttcaaataca aaatccaaac atgagtataa
     5821 tcatagacaa acgaaagatc gattgtgtta ctttagtaac cgtgcccaaa tagggctata
     5881 taatcgatta aagatatgat aatattatgt actacccaaa atatttggct ttcaaacatt
     5941 aaaacattaa atttgggtga agtaggtggc cggacgctaa ggcattacca attgttgtcg
     6001 ctgtcgagcc agcaaccatc atacaacaat tacaatgcag aaattagtca ttcacctatg
     6061 tatgatatta tcactcatcc taccgtaaag aaactaacaa aattatgtta taatactgtg
     6121 acccacgtaa acaactggaa aacttcacca caaaattaaa tatcttgaaa ggacatgttc
     6181 gagggtacga agacaatacg cgataagatg caaagcaaat gaaaaagggc tcaatgaatg
     6241 atatgtttgg acgaaacaag ctaaataaac cactaaaggt tcgctgtcca ttttgcaaac
     6301 acacatttcc accagaaatt attcagatta ttggaatcac ggaggaaccg attaattttg
     6361 taatattagc atgtcctaat gcgaaatgca ggacaatact tgacattata ccacatcttt
     6421 cggtcggcac ggtcggcaca agcaaacaaa ataacaaatg aacaacttgc tgaaatgatt
     6481 aatagtttgc taaagaccca tgatagcgaa ctggtgcgtg gcgataaatt tgagagtgtc
     6541 taattatccc atacagtatc aaattgttat ttaattgaac gaactgattt caacgttgat
     6601 attctaattc ctctttgtat attaccattt gcagataaga cattcaacaa atagacgctg
     6661 ccttactgga atgcatacgc aaggcaataa tgtgcagaaa gaaattattt tatccctgtc
     6721 cagtcgtagc aattcccgca cttctcacac ttttctggaa aatttttgtc ttccatagtg
     6781 atcctgaaaa agcatcttcc aaaaccaggg aacataaaat agggagtgct acaccaaaaa
     6841 tctcgtctat tagtctgtat ggccattata tcaccgataa atgaaaatac tgcagagaag
     6901 tcgaaaagtg ggctgctcag ttgattgcta ggtagaaaca gtcttcccat ccagatgtct
     6961 cttaaggaat atggtctata gcgcatgatg taactcatgt tgcagcatgg tctagtgaaa
     7021 caattaattt aaagatatac agacagatac gcaaaaatgg agagggtgcg gtttcagata
     7081 atgccggtat cgctttgctg caatcgtgcc tacacattcg gacaagctat tcaatatagg
     7141 cctgatcgaa tataaaaaca ggccatatgg catcaactat tttgcttgac gtgactaaca
     7201 aaatactaca cactaatgat atggaataat ctttatttga tacttttacg atatattcca
     7261 ttgtccttca catcacgcat aagcttccat cataggtgtg taggatagac acgacttgca
     7321 aattggttcg ctcatggcca atttgcaatt tattattaat catttatacg taaatactat
     7381 ctgtatagcc cctaaatcgt ttaatcgcca ttcgttatcc tggggcatct ggattttctt
     7441 tgatatcatc ttttactaga acttgaagga gaagtcgtaa ttgtattcac tgaacctgta
     7501 tttgttctta tactcgacgt agtagtagcg aaggaaaatg aaagaccaga atgaggttca
     7561 agtttgcaga atatgactgg cctaaatgtc cgatccataa ccacagtccc actactcaag
     7621 caagtgatat acccataact attatgcacc atcccaaagt gctcgtagcg ttattatcgg
     7681 gtacatagaa cggttttcta gggtgttggc gtgtctatag attaaattac cttcagtata
     7741 ttattatatt aaaagataag ttttatgaaa aatccattca agagatttgg cagccctaac
     7801 ccatccaaat attcaggatc aagatcatct aacaaattaa taattgtagc a
//
LOCUS       DAOR01000285            2596 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_41360, whole genome shotgun sequence.
ACCESSION   DAOR01000285 DAOR01000000
VERSION     DAOR01000285.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_41360"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2163..2459
                     /estimated_length=297
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          753 a          412 c          385 g          749 t
ORIGIN      
        1 attctcacaa gcatttgcta gtcaggtaga atcaaaagta gttgcgttcc aaaaatttct
       61 aacagaacaa aataatctat tggcaataca ggtaatagca aaccaaacag ttgtacaagc
      121 tcaagcagca gcaagggcaa atgtagccaa ggccaacggc gaatctcagg caatccaggt
      181 tattacagta caattaaaac aaagtccgac atatcttcag tggctatcaa ttaacaggtg
      241 gaacggacaa ttgccttatg ctctcggaag tagcgcgttt ccttttttcc aactaccgcg
      301 agcaataccg ttacaaaacc aaactaaatg aaaatctcag gctaaaatta atttttttct
      361 gaatcttact caaagaatac tattcagatt ggtcattaat tacgtatatt gaatatgtca
      421 tattgcttat acgaatcctc tattttgtta tttgaaatgt attaaccagt tgttgtatag
      481 ttggcaaata cttatcatat tcatttaatc ctgcttcgta tactagaata tacactttat
      541 ctcctttcat tactccaatt tccaaatctt tgaaaattgt gttttcatct gcatgagtat
      601 aaatcgattt gtatgcagat aatccagcaa gaacactatt agtgtttgat gatataagtt
      661 taaagtcttt aagagattgt ttcaaatcac cgagactgcc gtttgcgtat tgagctaagg
      721 ttataccttt ttcatctgaa atatcatcta tagatatgtc aagactcgca tttgaattga
      781 tagttggagg aaggaacgaa acggcgtcag tttccgagcc cagctttgtg ccattttctt
      841 gcttctccca gttggaggga tattgtattt ttatcccgta gctagaattt tcatatgtca
      901 ggaaattagt ctgttgtgca aatgcaatct gcttgtgaaa tgtcggagct aaagataaac
      961 aaaatgtaat cacaattaat gccgatattg ataatattag cttactcatg atagctttaa
     1021 cccccaatta tcatcgctaa attccactgt aatcaggcca tataatctaa atgtttttag
     1081 caaaatagct aaatccaagc acaggctaag ccagcttaca ggatttgaat tcagaagcaa
     1141 tcttaacatc ccatcactag gacttgagaa gaagagtcgt ctaatttttc atctattgtc
     1201 ctcatattaa ttatctgtta tcagagtgat ttggaaaagg ataagactag ctctatttac
     1261 ccttatgtct aaacctgttc gattactaat gtaattgtga ttcttgcttt aatcacctgt
     1321 aatctacgat agatcaaaag tttgataatg ttgtcggttt agctaaacta gcgacacccg
     1381 tttaaaaata aaatatctcc caatttaata ctagaaagaa taatatcgat aataatattg
     1441 aatcacgttt tgttgcgctg caggaatcgt atcaggaaaa tattagtaga atatcttgca
     1501 gacaataaaa taaagtacag ggatggtgat gatgatggta catacctaat gactgattct
     1561 attcctatca gacaattttt agaaagacta tggctaatag atgagaccaa ctcctattat
     1621 ctaataatcg agtatttgta aatcgtgtcc cagaaataaa gttcataatc aagaattagc
     1681 cccgcagctg cacatatact cttataactt tgcggaatgg tggaatctat taattcttta
     1741 gatttactga caaagtcttc agaaagagga gaagcaaatt tgtccaaaaa tacacaggaa
     1801 ttgtcagtaa agccgtagtt ctttttcaat atagagctca ttttactaca gttggctccc
     1861 cagactggaa ggtctatgag tagcgcagta agaatttcag catcagaacc ataaactgcc
     1921 aatctagtta agtagttggt ataagcttga catcctgcca gttgttcata agcttctatt
     1981 ttcctcttat cgacaaccaa ttcgtcagcc attattgtta gatttacgag agcatccaat
     2041 tctgcagcta gacaatcagt aaacagtatg ctagctacat cacttgatgt ctttgatatc
     2101 ataaaagcaa agtttcttct atcatttgaa attatgtgat attgttcaca gacaaatatt
     2161 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2461 atctccttct tttctaatac gtacaagtat ggatgattac ggatagcatg tataatgtta
     2521 gatgaattgt cttgtatgcg ttcaataaaa ttcgaatcat tctgatgatt gctagccatg
     2581 gatgctgtct cttata
//
LOCUS       DAOR01000286            9056 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_30331, whole genome shotgun sequence.
ACCESSION   DAOR01000286 DAOR01000000
VERSION     DAOR01000286.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_30331"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    423..671
                     /estimated_length=249
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2759 a         1484 c         1673 g         2891 t
ORIGIN      
        1 tcctttagtg agtagtaacg ttggaataaa ttttactgaa ttatggccgc caccatggca
       61 gcagcccagg tggatacggc acaagtttcc tcgaaggggg caggttgtca taggtgaaaa
      121 acaagaagac ctgataacga taggatttct tgtcaaattg aaggaaaagg cgatcattca
      181 agactaggta ttcgaaattg gcagtagcat caaaaactaa gcgaaaattc aacttcaacc
      241 cccatatcct ctcaatagct caatttccgc tcaatcgacc gttagaatcg gcaacatatg
      301 agaagctcgg tgatcgagct taacctaact tagcttttat gccagctcgt caggggaaaa
      361 aactatcaat ctctcaatct gcacgccaat atagacaaat gtttgtggcc ttcctcccgg
      421 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nggagggacg gaggctacgg aggtaatctc gatatcggaa ggagatgcaa
      721 gcaaaacaaa aaaaacattg attaaattac gagaaggcag gtgtttaatg tcgatgttat
      781 ttctgggcgc tggagcttca aatgcatttg gtataggtca ataaagggat ttcacgccaa
      841 aagtagatga aatacttgat agtaatggct attctgatct tagaaaacat atccatgcag
      901 tatttgagag aagctaacaa cgattcggca tatttcgtcg acgaatatga gatagatatg
      961 aaggttatta tttcagtttt ggatgcaatt gtaaatccaa ctcaaaaatt aagggactta
     1021 ggtccatcta tgatatacat gagtgaatta gcagacaatc cttattcagg agaaacacca
     1081 acaatgagtg aactacatat catcaaaaaa agtatagagg atttaattat caactcgtgc
     1141 aacaacttcg atttcgaaag ggcaaaagaa tactacagat ctttacttaa ttttgaaaac
     1201 tcagtcacga ttttctatgt caatgcaaaa aatgaacatc cacgtattcc catattttct
     1261 catactgtta caaccaatta tgatttagta cttgaaagat actataagga atgtgataca
     1321 gacaggacac tcccagccaa atacttcctt cgcagaggtt ttactagacc atatgatgaa
     1381 gctgaacccg agttaaggat tggagaaatg ggccaaaaat tagtattagt tgagaataat
     1441 ggtttgtttt aatgaggccg ataacactaa catcaaaagt ttagggactg ccgttaacgt
     1501 acatggctac caatacgctg attggaagat gtagacatga aggaagtagc tcaatggaga
     1561 cgcaagaaga atttgaggag gatatggatt atctatatga tgtagacttc gagtaatggt
     1621 cattagattc cttttctgtt atcttagaat atatagtcat gtctagacta gactagacct
     1681 agagctgacg aagttgcgta aggattcaaa aaacttgatt ggttgttgat gatgtagtat
     1741 tattatcatc gtatatacag tcttgccgat gggaaggatc gttattctct acttcacgag
     1801 ctttcaaatc atcacaggga ctatgatcta tgttattagt cagtcgcttg agccgctagt
     1861 aggcgatttg atgtggtgac aaatattaac gagttgtcac ttatggcaaa gaaactccgg
     1921 tagcttttaa ttgactcatt acatatctga cgtgcgcagg cgatacccta tctatagttt
     1981 tatcttttgt gtcgtataac attgcattta tgagtcccgc tatcatgtcc gcatcataaa
     2041 aagcagttga ttgtgagagc aattctgcta ctttcgatat gtcagcgcca ttagctatct
     2101 ttccacttgt aattaatcct ccaaattttt gtactgattc atacactccg atgttgtatg
     2161 ctataaatgc aattttctca ggcggtagaa catcatcaat ggattccatt attatcttaa
     2221 atagtcattc cgaagataat aatttagtta tagttataca tgcagaaagt atatagttaa
     2281 catatcagga ctgcagcaaa tatgagctta ttccactacg aatgctcgtt atttggacga
     2341 ttttgataca gtaattatga tatattcttg cctctgccta ataaataata atgtattcgt
     2401 catgcggcta gctggtgaca tgcaattata gcagtttcgg gacacatgag actttatcgt
     2461 ggaaaaagta gattccaaaa agcgtagtaa aatacattaa aatccattaa acctgatcta
     2521 ctgattaaga acgtaaatgt agtaagcctt ggctaaatca gaaaaataaa gttgctcagg
     2581 tttcatgagc cgctatatat tctgcaaatt tctgtataag taaagttggg ctatccatgt
     2641 gctgaaacgt catatgttca agtatatcct ccttggtttt aaagcccgtt ccggaagcac
     2701 aaacagggca gttcatctga taaaagtcat taatacttgc gatatttaat gattttcaga
     2761 ttctgataga gtccgatatc attttttgcc gtggtttctt aggttagaaa ccctaatctc
     2821 aatccttgtt cgctacattt aactagtcgg gttcgttccc tttcaaatat aatacttggc
     2881 taaaaaaaca ttccacttag gaaattcttc gttatttaaa taatattttt ttatattaga
     2941 cacaacctat atttctatga ataagaaagg gatgaataaa tctgacctga tcactccccc
     3001 gattcgaagt agaaaagtag ctgtcattac tgcctgtgtt gtaatttttc tcgttgtagt
     3061 ggatttgcta atgacgcgtc agattgtacc ctataacaac acttcggaaa ctgtcatgtt
     3121 catacttact gtagttgttg cttatggagt aggatcttgg attcttttag gatatactaa
     3181 gcaagcaatt agcggactta gagcgaaatc tcacttcatt aatataatgt attgggcagt
     3241 aacgataatt cagttttgct tattagcaat tttgatattc gtgatattta ataacagcag
     3301 cagaattcca gtcttatcag tttatctgat tagttccttc tcagccttaa gtatcatcgg
     3361 aataatcagt ttcaaattct tttcatggta cagccgaggt aaaagaaatg tcatggtctt
     3421 gttttttggt attgctactg caacgtttgg cttttcaata ttggaagatg catatactaa
     3481 gttaatatta gtacaagtgg ttgaagaaaa atcacctcta ggagcgatac ctcagtcatc
     3541 ctttctgtat ataactgata aaaaatatca tgccgaaata caatataaag tagtaaattc
     3601 gcacacgact acactgtgga tcctaccaag ttcactgacg tcgttcaaga acagccttga
     3661 ttacctaaca gtatttcctt atttattcac atggctggct gttgcgacgc ttttacgtca
     3721 gtattaccaa acgatcagaa taggaaaact ccctcttaaa ttttggatca ttctatctat
     3781 tcccatggca ttctatctaa ttggaagtgg tttgatattt catgcgtacg atccatatcg
     3841 attttacttc agaattctat tcagggctgg tacgataggc agtagtgtgc tatttggtct
     3901 tgctttttat cttatcacaa gaagaacagg agaggcagtg aatgtaactg ctgaaaaggt
     3961 gaaagattat cttactattt cttcgatagg agttattcta attggtattt cattttccac
     4021 ttctgcgcta cagcaaactt atggggtggc agctcattcc ttagtattgt tagcttcgta
     4081 tttgtttaca gtaggtttat actcttcggc aatagcagta tcacaagata gttctttaag
     4141 aacatcgatt cgagcttctc tgttagagct agtagataat attggaacag cccagatgga
     4201 gcaggaaata gagaagagag ttctaaaggt tgcgagagaa caacaagagg ttctgagaga
     4261 acgcagtgga attgaatctt ctcttacaga aagagatatg aaagaatatt tggagcaggt
     4321 aataaaagag gtcaagaaag aaggcccaac atcatgaata gggactagag atagcggtat
     4381 caggtaatga caagaaaaac gatattttat tttgaacaag aactgtcttt acagcgtttg
     4441 tctaacctgt aacttgttaa ggataatact ttcaacttgc ctctttgagt aattgctata
     4501 actgaagatt cattcccgtc agtgcttacg aaaaaatact gattacaatt tttaattttt
     4561 tgtactctgc ttaattacgt gaagggcggc atatgaccta gtagacgtac ttatattctt
     4621 caataatatc tatctaggtt accattatta cctgatacat ttattgttct tctatttttc
     4681 ttgtcattat tttcgtgtcc tctcatatct acatctgtgg cttttcttac catctttaga
     4741 gtgctggcat catgagtttc tgaaagatat atagtatata tcaacgctgt atcttttcgt
     4801 tcagtttttc ttattgttct acctattctt tgtactattt ggttcatatt agatgatgtt
     4861 gctaatatta ttgccactct gacttccggt acgtcatagc ctatttctaa tgtatgtact
     4921 gagagcagtg gaaagaattc ttttccccat tgtgccagta ttttttgtct ctctgttgat
     4981 ttcagtttgc tctcaattag catggattct attcctttat tttgtcgtaa catttccttt
     5041 agtttttgta tggattctat agtttcacta aataccataa tcctctcaga agaatgtttt
     5101 gttgctataa tatctactgc tgctaatagt ttatttttag cacaattaat cagattcttt
     5161 ctctccttaa catttgcaaa ccatgctctt gctagtccag ctgtacgtcc tcctgttctt
     5221 aacagagagg atatacattt aggatcagaa gtattaagat acatggagat atttctgatt
     5281 ttggcagagc attcgtcata gatctttttt tcatcatatg ttagatcagc ttttattgga
     5341 ataacaacag gtcttgctaa tcttttatct ttcacagcat cttttatcat atatcttctg
     5401 acaggtggca gcaatgagag aattgtattg tatttaggat cttgttcatc tattgttgcg
     5461 gttaagccta gtaatgcttt tttaggatct gctgctgtta caaaatcaaa tacctttctt
     5521 aaagttgtag cagtgtcact taccagatgt acttcatcaa atatgatcat gttggagtta
     5581 ttaattagat caagattgct aattgtgctc tggtatgtgc ttattgttat ttcacgtact
     5641 tctttacgtt ctccaaaata tgctcctatt ctctcttgag gaataccata gcttgttagt
     5701 ctgttaatat tctgctgtac taggataatt cgcggtacaa gaaagaggat attgaatgaa
     5761 ttattattta tattagaaac gttattgcct tcgttatgta atagtgcttc acatgctctt
     5821 tttgcacatt caaaagctat ttctgtttta ccggtgccag tactgtatat tatcgaacct
     5881 ttatagccat ttgttatcca tgcatcaacg gcctcagtct gatcctttgt aagtttaaat
     5941 ggaaatttta gtgaggaaat tgctgagatt attggcggtg gagatgttgg tgaagatgct
     6001 ctcgtgctcg gttttgagtt caacatgctg taatatccta tcacaatata aacatgtccg
     6061 gttgaaaaat attatttgtt aagacatttc gcaacttgag acttctttag ttagtcttca
     6121 agttattaga gaacaaaaac acgaataaag atggagtagg ctagaaaatg gtacaatttg
     6181 gatttaaaat ccctcatatc ttgatagtta tttgcgctat gttagccgct gcattagctg
     6241 acaaagcgtt aaatggacca ctcactttct tgcgcattca attcttagag catgatttta
     6301 ttaaaactaa atatcaatca ggaccaatct tcaccaatgt ctttccaaag ttctcgccat
     6361 tgaagagagc catgagtgtg cgtaaaaata aaccgacacg ggcagggttt gcagcagaac
     6421 ttcgttgtct gtatgttctc tggggtttta atcttctaaa tggtaacttt cgctggttgg
     6481 tatataatct gtagtcctag tcaatattcg gggtcttaat cctcgtatag ctaacaacta
     6541 agcaatccaa aaatggatta tgcctatcct gtacgcaatc ataaagtcta atataagaac
     6601 atgaggagta caatgtaatg caatccatct ttttttggat tattattgtt gatatctgac
     6661 gttatgcgcc tatatccgtt cttagtttgt ttccatagaa tgcatgcgta tctctgatct
     6721 caaatttgta aatatagcag aattcttcta agagtattct gtacacgttt cctagaaatt
     6781 gggagaattt atagccatgt tgaattgaat tgtattgtat tattaacacc tcattatccg
     6841 tatttgctgg actcttgcta tagcaaaaga tttaatcaac tcaaaatatg cttcaagact
     6901 aggttttcca tgcagaagaa tagatgctct ttgtatgtgg tgacgatttc ttctgatgct
     6961 ttaattattt cttctctggt gtatgactaa atgtagtaat ttgtaactat tattaaatgc
     7021 aataattcca tgcgacaatg ataagaagta gaagaataat tgaatattcg tagaaattta
     7081 taaatgtcac ctctcattaa agacgtacta gcgtgattga tgaatttact aacagtgcag
     7141 gagtcagaga agaggaaaca cattcagata aaccaaagaa aagccaaagt atcactttta
     7201 gattagattc tcatacaata gatgaactac agcgtgaagc agatcaaaat cagatatcac
     7261 tcaatgtttt ggtaaaccag gtcctgaaaa gatattctga ttgggataga tatgaaaata
     7321 gaattggcat gatgcctgta cccaaagcga tgctaacttc tttaattgat aaagcagtag
     7381 atatggctaa gaagaatgga ataaaggaag aagatattga acgtgtcaga gaccaaataa
     7441 taaaacaagc tgctgagatt gcatttaata taattaagga tgctgtgttg tttatgaaaa
     7501 agcaatataa tctctgggtt gttcttgcag tattgcagga gtatatgaaa gtatcaggca
     7561 taaactcaga ccatagaata gaaggcggaa gaaaacatgt atttgtaatc cagcatgaac
     7621 taggtgagaa ttggtcactc tttacaaaag agctgttaga attgatattt gaaaacctag
     7681 ctaaagttag agctcaagtt aatattactg caaaaatgac tgtagctgaa gttattcttt
     7741 aactacccaa caatattgat aattatctag aaaaatgtga gggataacat tcataaaaat
     7801 tataaaattc tcagtgcact gcatacatac tagttaagta tctgcttacc ttacttggtc
     7861 tattacaata ataatggctc acgtttcgaa atccggtcat aataaaaggg gacgagtaaa
     7921 accacttact gacgaagata tagtacacga atactttagg ctcattaaaa ctaaagatat
     7981 gtatagacta ctcgaccttt ttgcagaaga tgccgtaatc gacgaaccat ttagcaaatc
     8041 agaagctggt aaaggaggat tacaaggaaa gagtgcaata gagccttttc ttaacgtcgc
     8101 aatgatggtt agtgatgggt tgagagaaga catcgaggtc gaaaaatcaa aactgagaag
     8161 cgataatggc atcaagagag ttattgcaaa tgtgactttt gagagaggag gaaaagtaaa
     8221 agccagattc acatttgaat taacttcaga agagaattac aattctcaaa agcagaaaaa
     8281 gattcagtct ttgcgtattc agtttgttga gtaaatctat cgtatgagaa aaagacttga
     8341 tgaaacaaaa gcgtatacag ttcttaatcg ctgattccat tggattacaa gtagtagcta
     8401 atattataat tgaacaaagt tcgtatacac gcgtatttca atatttagaa aatgtgcgca
     8461 gcgcattgtt gtccccatat tcctttccta attgttgtac ttccagacat ggttgacact
     8521 ttttgtcttg ttttagtgta tcattatcgg cacctaatca agcgggaata gtgggaggta
     8581 tatgacatac tttttcttgc tttcttgatt atcatttcgt ttgcattcct ggaacatttt
     8641 tgcgtgcgca ggtaggtata atgcagtata atgcactgaa ttcgttgtga ttataatcat
     8701 gctccacgtt gtgttatttt aagaacggag acgagaatgg gttgtaaaaa catctgcaca
     8761 agatacaagg caactaaaca tagaagaagt agttactatg cacaaggtta caaaagatgt
     8821 tctatgtgcg acattttcat agaatgggat ggtacaagat gtccatgctg tgatctagta
     8881 ctgagaacag tcgcccatca gaagacaaaa aagttccgac tttgaatggg aagatatttg
     8941 aagggctttt agacacggat agctgacgcc aatttacatc tgagtctctc tacattcagt
     9001 atctgtacta tctctcgggt aactgcttca gctagtctat ctttttctgt ttgtat
//
LOCUS       DAOR01000287           28705 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_9967, whole genome shotgun sequence.
ACCESSION   DAOR01000287 DAOR01000000
VERSION     DAOR01000287.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 28705)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28705)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28705
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_9967"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1043..1224
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6833..6995
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24492..24761
                     /estimated_length=270
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8191 a         5890 c         5526 g         8483 t
ORIGIN      
        1 aaatattcga gacgtctgta gacactataa gtttttaatg gaattaaata tacatcgagt
       61 cagttgcacc caaggtctga ctatttacgt gtaaataggc gcatattatt agagaatgtg
      121 tgaccgataa gtttaacgtt gtcgcaactg gtgggacttt tgatgagata catatcggcc
      181 acctggctct gctctctaaa gcatttgaga tcggcaaaag agtagtcatc ggggtttcta
      241 gtgatgaatt tgtggatatg gtaaaaggga aaggcaagat cactcatact tacgagcaaa
      301 gagtagccaa ccttcgactt gtaattcaag cgaatttcgg gcatgtcatt tatgagataa
      361 gtaagctcaa caccacatac ggacctaccg taatttccgg tgaagtaaat gcgctcgtcg
      421 cgagctccga aaccgcgaaa aagggttcgg aaatcaatga aattcgatct aatcaaggca
      481 tcaaacccct cgccattgtt atagtggaca tgattagaga cgaagacggg gctcccattt
      541 catcatcacg catacgcaca ggtcaaattg atcctcaagg taaattatta aaaggtgaat
      601 aaaaaaactc aacatttttt gattgatacg gttttatcct attcctgaaa ggtagtttga
      661 ttgcataatg tttcatgaaa agccatttaa ttttgccacc ctgagaatcg gaacgggtat
      721 taagaagatt aaagaaggcc ttgagcctga gatccttgca tattggtata agaggatcga
      781 agacaaggcg attgaatcgg ctccatccca cctcaaggag aaaattcaca tcacacagga
      841 caggattttg tggatgaaat ttagagtgga catatccaaa agagttgttc cctatgttat
      901 gcaagttata gatgagtaca tccccatgat gccatattca actggtctct attttagaaa
      961 agttcagcaa atactgatac aagaaatgaa caaagaactg cgttaattaa ttgattatga
     1021 attccagaat gcgtcgtagt agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnattcca gaatgcgtcg tagtaggttg catgatccca
     1261 ataaaaaatt ttatgccgac ttgctcttct taagagaata atattcaaga tgttctgtgc
     1321 aatagagggg ttaaattgtt ttatattagc ttataccttt cgagcggtgc caatgttccg
     1381 tcttcagttc atgtttgtaa tccggacaat gtgagaatca tttagagctt aatatcacag
     1441 agcgaaaaat tgcaagtatg ttattgtttt tgtaaatatt caatgtagcc aaaatctcct
     1501 tttgactcag caatacgagg tccgaatttt caaaatatgt acttttcttt gtgatttttt
     1561 gcaaagcagc ttctaaatgc ttcattgaaa ctgccctctc aagatatcct tttcctacag
     1621 tttttcgcaa aagcgatgca ggttggtctt tctgcttcgc ttcaacgaat agtgatctct
     1681 gtcacgtgat catcgaaatt acttgccgaa attctcttct tttgcacaag aacatcttct
     1741 ataaatgact ttaatctgac agctgtgtcg cgatccttca ttataatact atgtgacgac
     1801 ttgtccttta ggatgataac aacctcattc tgaactatgg ccgctatccc gtgaaggaag
     1861 gtgccgttat ctttttgtgt cgctaaaaat cctgctagct tttcagcttc atatttatca
     1921 tcgcactcaa atttttccac attcattagg tgtcactttt cataagttgc tccatcgaac
     1981 atgaattcag cgataacatc ctttgctttt tccctacgct ttttgaattc cccgagaaat
     2041 ttttgagatt cctttgataa atcgctcttg cattcaccgc aaagaagatt tccgctcgag
     2101 cattttagca accgctcgtc tgattgtctt tcagtatcaa acaaatatcg taaataccag
     2161 aaaacaggac aacctaatgc attgccacct agttgtcttt gaagcgctac tgtcgcttga
     2221 cctcccgtga aagcagcgga aactttctta tctatgacct ccggatcatc aattgtgaac
     2281 aaggctgtct ctggtttaga tgacgacatt ttgcctacca ttccaagacc tgggagaaac
     2341 ttactatgga tctgtgcagg tttataatac cccatgcgtt cagccacatc cctcgttact
     2401 ctccagtaag gatcttggtc tatggcagcc ggaatgagaa ccggggttgg cttattttca
     2461 attattgatg gtaaaaagca aggtgctgcc tgtattggtg ggaatccaat catacctata
     2521 ttggtcgaat ttgagaaacc aaataccgcc tttgcagtag agaatgtgat tcgctttgca
     2581 atttcggttg cgatagggta aaggtgctgt atatgtcttg tatctaggat aatcttagtc
     2641 ttatcgggat taaagccgac agcaattata tccaaaatgt tctcataagt gtaatgtcca
     2701 atctcttctc tcgaacgatc ctgagtctgt agaaattttt catcatccgt tagttgaaag
     2761 agtaatttcg aaccaaattt ttcttggata tatttggtaa atagccaggg catcatatgg
     2821 cctatatgga ccattcctga tggtccccta ccagtatata gatagaaact ctctccctcc
     2881 tcatgcttat tcaagatcca atcaaggtca cgatgagaaa aaaaatactg aagttttaac
     2941 atcggatgaa cttcgccggt cacttgtttg attctctcta ttatctcaga ggttatcggt
     3001 tgggtaccaa attgggctat tagtttgttg taatctattt ctccttctac ttcccatgga
     3061 gtaactttga aatcgtgttt tgaattatta gtactattac catcctcgtc attactagta
     3121 tctgaagatc ctttactatg attcaactcc aacatgaagt agaaacctta aacgtttaaa
     3181 aagtcttttt tctctgacat ctctgccctc agatgactaa cagcaacacc aatagccccc
     3241 tccatcctct cgagaaatcc atactagaag ctcttgtcgg tcgcaataga caatcaatcg
     3301 aagagctagc gaaggacact cgtctgggta tcgatcagat aagacgtggc atagaatggc
     3361 tgaaattgaa aggcttgatt catcttgacg aaatgagttc aatatcccta gggaccagtg
     3421 gataccaagc aataaagcaa ggtttgccag aaagaaggct tgtagattac ctaaaaagtg
     3481 gcaagaatcg gattcaagaa atatcaaact caggtttttt ctctcaaaaa gagagccata
     3541 gtgcgtttag atacgcaaaa caaaacaact ggatagaaca gcagcgagat tcaatcggag
     3601 agaagagact tgtccgaatg ctcgcagccg acaataactc tgccgaagaa aaattattga
     3661 acaagctcgg ggaacaagaa aatctcaaaa tgtcggatct cacgtctgag gaaaccaacg
     3721 cctttaattc gctgaaaagc cgagatcctg gatatgtgag ggaagacaaa ctaccaaacg
     3781 aaaccacagt cacgctatta gaacctggca aaagggcgct atcctcggtt gccgaagcag
     3841 gattgatggt agaagcttta gctgagagaa aggctccaac aggcaaattc aagagcgtta
     3901 atctccctcc gatggacgtt caagccccgg tacgttcttt atttccggga agaaagcacc
     3961 cgatagcgaa cctcatagat gaaataagtg aaatttttgt tggcttgggt ttcactgaaa
     4021 ttgaagggcc gatcacgcaa tcgagttttt ggaacttcga tgctctcttc actccacaag
     4081 atcatccggc aagggaaata caagatacat tctatttggc acaaataaag caggacaaat
     4141 ttgctaccga ggatcaagtt cacaaaatct ccagcgctca caaagatggg tggaaatatg
     4201 aatggaatat tgatgatgca aaggggatcg ttctaagaac acacacaaca gccgttacta
     4261 taaagtatct tgcagataat aaacctgaaa acgccagaat attttcgatc ggtcgggttt
     4321 tcaggaatga aaaggtatca tataaacacc tcgcagagtt tactcagatt gaagggatag
     4381 ttaccggcag tagagtttcg ttacaggatt tgatgggttt gcagcttgaa ttttacgcaa
     4441 gattaggtat taaaaaaata aaattctggc ccacattttt tccttataca gaaccctctc
     4501 tccagtctat gatatataac gaaaaactgg agaaatgggt cgaattattt ggaatgggtg
     4561 tttttaggcc tcaagtgacg aaagcgctgg gtatacataa tccggtgctt gcgtggggag
     4621 gcggtctaga aagaattgca atgttacgtt ttggactaac cgatgtgcgt gatctctatg
     4681 agaataagtt aggttggctt aggagtattc cacgatgcca gttgtagcat tcactttcga
     4741 caggttgaat aaattcttcc caggaaaaaa attagatgat attttgaaga tcgttccatt
     4801 tattggagtc gatattgagg gcgtcgacaa tgccgcggtc agaattgagt acaatcctaa
     4861 cagacctgat tttgcttcag attatggaat cgcgagggcc gtaaaaggat tgttaggaac
     4921 agaaactgga attcccgaat ttaagttgtc tggaagaagt ggatgcgcta tcaaaattga
     4981 tatgtccgca agacagatca ggccatacat cgtatcgttg gttgctagaa atggtaaact
     5041 tgatgacgaa actataaagc agctaattgc aatgcaagaa gacctccaga atgtgattgg
     5101 aaggcggaga ttgaaggcct caattggaat tcataattta gatgctatca aatttcctgt
     5161 tacatatacg acagtgcaag aagatttttc gtttattcca ctcggagatc cagataacca
     5221 cacaatacga cacattttaa aagaattcga cgttggcaaa cagtatggat acatattaca
     5281 aaaatcaaat gcatatccga tcatccaaga tgccgaaaat aatgttttgt cttttcctcc
     5341 gattgtcaac agtaatttga caagaatagg tacagctaca aagaacctct tcgttgaagt
     5401 tacggcaaat aatctagagg tagcagagga cgttctttca gtaatggcta tggcactata
     5461 tgatgcgggt tttgaaattc agacagtctc gactcataat tctgatggac gaaataagga
     5521 gacacctaaa atggatcctt cctatatcaa agtcgaaacg agctatatca acagaatact
     5581 tggtctgaat ctgaaagtta ctgatatttt gaagtgtcta aagaaaagta ggttggacgg
     5641 gaaagtcatg tctggcaaaa atatcaagtg cactattcca cgatacagaa ctgatataat
     5701 tgatagtacc gacctggtag aagaggtagc tgtgggatat ggtatataca atttgagggc
     5761 cacgatacct tcatccgatc tttcagggaa gcggaattat ctctcagttt gcttgtatat
     5821 agtcagagag gcgatgactg gacttggaat gattgaagtt gtgaatttta accttgtcag
     5881 caggaaaata cattatcaat taatgggtat accagaacat gataatatct tgacggcaga
     5941 gaaaactaaa agtatcgaac atgaaatact aagggagtca ctaattcctt ctctaatcca
     6001 aacattgtct cacaatatac atgaagaata tcctcagaag ctatttgagg ttggcaaagt
     6061 ttttcaacgg ggagaccgaa taaatgaata ttggaatctg ggtgtagttg tcgctcattc
     6121 taaagcaaac tatactgagg gaaaatccat tgtgcagacg tttttgaaga caagctttgg
     6181 caaagagatt gttacgaaat ccaatgtaaa cgccatccta gttcagggta gatcctctga
     6241 tatcattgta gatggacaat cagtcggtta tattggagaa cttaaaccaa tggtcagtga
     6301 taattttaag ttaagggttc cagtagttgc cgctgagcta aatctatcac aaataatgtc
     6361 tgttagctga tctttatatt tgtgtgcgtt tatacacgtt gttgccgaca gtagcgcgtt
     6421 attacactga tgccgagagt tcctagtttc ctcgacatac gcgtattact aggcaccccg
     6481 ttatagagag tacacgagga ggagtacaca ttcgcgtgta tggctaccca tgaatacgtg
     6541 tgggtcagag gcgggggaca actcttttta gctaccatgc gaaataatta ttaaaaatat
     6601 atcttattcc aggttatcga agaatccaag gtcctgtgaa aacaaacaat acacaatttg
     6661 atgtgaggat ccgtctggtc aaatcaagaa caatcttgga gcacatatca agatttacca
     6721 agacttttga tgatattcct tgaagaggca cttctgagag atgctcgctg taggtggcga
     6781 tggaatcata attgcgaact gacttaactg gtcttaggca tgtataatac acnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtctt aggcatgtat aatacaccat
     7021 gtaaattttc gttggaggag ggacgagtga gtcgcagtac ttacttacag tgggttcaat
     7081 gggcgtggag ttaggctgca tcaatctttc cgatctcaaa gttgacattg tcatgctaga
     7141 gaattaggca ggtgttaagc tcttagacca gtacgttaga gattttaata ctgttttcta
     7201 cagacaaggt tagaaaaggt tcggtaaaat taaagaagag atatcaacga aatggtagag
     7261 acgaaccgat tggaattcta tccccaaaga caattgctac ccaaatgccg acgaaaagtg
     7321 cgtaacagaa ttcaaatgat gtatacgttt ttaaccagca taatcctcgt cgagtaataa
     7381 ataaatcaac atttcagaac gcaatatacg tttgatcggt gttaatagat attgacatcc
     7441 aatcggaatt aatagagata tgccaagcac tattgtgtga gatcaactat gctctttctg
     7501 atgatcgatt tggctttagg tgaaatattg tattgctcca agatcttggt gggctctttt
     7561 accagttgat ttctgaattc tggttcactc gctgctctaa gcagaatgtc gctgatggtc
     7621 tctgcgtcct tctgtcttgg gtccaagtac gtgcacggtt gcaaaatcct agtatataat
     7681 cctaatgcgt gtacaacagg caaacgtcga ctttgcggat aatcatggat tcagagattt
     7741 gtgtcggggt gattcgtaaa cttgatatga actaattcac cgttcataaa cgtgcaaaca
     7801 cagccattgc tgcttcagag tagagaaaag tggtccataa atggaagttc taggatcata
     7861 ccacctgaac agaccctcag gatgaccata tcactatcta agcagattgg agtaacccga
     7921 cttgcggaca taacacacat ggataaatta accatcccaa attactctgc tgttttacct
     7981 ggaacagagg attacatttg ggtttatagc gggaagggtc caaccaaggc acatgcaaaa
     8041 gcgagcgcgt tgatggaatg tatcgaaaga tattcttcat taccaagcag tacacggcga
     8101 gatttcattc aaggaagtta cgatcaatta tcacagaggt acacggtcat ccatccagaa
     8161 gaggttgtag aacctttcaa ttttcaatac cgaaattaca tgacgatgga tttccttcct
     8221 ggttttgagc tgtttagcgg agaacgaatg ttagtaccag catctcttgc tttattcaga
     8281 tatactccaa aaccccctgc tttaaacccg tttgctttct atcatacaaa tggacttgct
     8341 tctggtaacg tattagaaga agcagtctgt cattccctat gtgaagtcat tgaacgtgac
     8401 gcaattagtt tggcggaact gcgagccagc gccatcccat tccattttat acgaactatc
     8461 acaaattcgc tgaaagcaaa acaatactcg gtatctcccg tacctgctga tcaatttatg
     8521 gacgattcaa gcatatttcc agatgttgat atacgtgatg tagattttga tcctatcaag
     8581 atgttagtta gaaaatttga gaaagctaac atccccctga taataaagga tataacttca
     8641 gatattggga ttcccacgtt caatgccagc agcattgagt ggctaactca cgattatgga
     8701 taccttgctg aaggccatgg tactcaccct gatgcgagga ttgcattgtt gcgtgctatt
     8761 actgaagttt cccaaactag agctgccaat atccaaggtg cgagggacga cctgcgaaag
     8821 ataaaatacg gacatgataa ttctgacgac aagagggcgt ggcaatttac gccatctaca
     8881 aataagatcc aattctctca ggttagatct tattttaaca gagatattct tgatgatatt
     8941 aaactgattt tggcacgttt acgacacgca ggcctaaaaa aagcaataat tgtagattta
     9001 acaattgcga aaataggaat tccggtagtc cgggctatag ttccagggct agagacattt
     9061 aaatttacaa aatcggttat gggttggaga gcgaaaaagt actttaaaaa aaatgaataa
     9121 gccaattgtt tttattggtc caagtctggc cattgaaaaa gccaggaaaa tctttaatgc
     9181 cgattataga ccacctgcga aaaaaggcga ttttctaaga cttctggcct gcataaatga
     9241 tgatgatcgt actatagtag gattggtcga cggcttgttt ttgcaggatt atcctcccac
     9301 tccaatagaa gtatttcaac tattaatcaa aaaaaatatt ctcgtcttgg gagcatcaag
     9361 tctcggggca ttgcgagcag ttgaactgga aaaatatggt atgataggaa ttggcaagat
     9421 atttgaattg tacaaaaaga acaaaattgc cgcagatgac gaagtagcgg ttacgttcac
     9481 tgaagaaact cagcatattc agtcagaagc gatgattgat attcgatata accttttcat
     9541 tgccttaaga aaaggagtta tcgacgaaaa cgctaaaaga gcagcagtca aagtcgcaaa
     9601 gagcatgtac tttccagatc gaaattatga agatatcata gatgaaacga aaaggcgaca
     9661 tccttcactt tcagattatt tggaatcttt ccgttcctat attagtaaga acagacaaag
     9721 tttgaaagag caggacgcaa taaaattagt aaactacgca aaaatgaaat atgaaggcga
     9781 aataacacgg aagtgaatct atttagttag aaaaaaagtc acacaacgat gtcaccataa
     9841 ctgctagaga cccatattgc tagctgatgt aagtaagcca ttttttgtta aaattgtcat
     9901 cccttccttg agtcactgct tcaaatttgc ttttaatctc catcgtaagt tgacctatgt
     9961 ctccattacc gattattcta ttatcaatgt cgcctactgc tcttattcca gccgcggtcc
    10021 cagtcagaaa aacttcatcg gctatgtaca tttcgtccct tgaaatatca cgaatttcat
    10081 aaagactacc atagtgttgt aaaatagcca acacgctatc tcgggttatc ccattgagag
    10141 ctcctgaatt taccggcgga gtaatcaata cattgtctct cactatgaaa atattttctg
    10201 cactagcttc gataactttt ccggcagtat ttagcatcac tgcttcgtcg aatcccatct
    10261 tcaatgcttc catccttgcg agagcagagt tcgcgtagtt tgctgtcgct tttgcattca
    10321 taggcattga ttttccatcg attctcatcc atgaagatac cattagtctt gctccttttt
    10381 gcggttcgct ttgtctaata tattcatccc acttccaaac tgctattgcg atagaaactt
    10441 tgttggggag tggattgaca cccattttac cgtaaccgta gtatgcgata ggtctaacgt
    10501 agtattcctc tggcttattg gcgttgattg tatcgatcac cgcgtcttgc agttgcttct
    10561 cagaatatcc aagatccata aaatacatct ttgcactgtg cattaatcgt cgtatgtggt
    10621 ctggcaaacg gaaagcggcg aggccatagt cggttttgta acaacgaatt ccctcgaaca
    10681 ccgctgttcc ataatggaaa ccatgagtga gaacgtgaat ttttgcatcg tcccagggta
    10741 cgagggatcc atccatccat attttgcctt cagtcatttt tattttcact aatacgaata
    10801 ctagttcgtt tagagtgaat tgccttaaca gcgtcaccta cattggtacc tttatcttcc
    10861 acaacaatca ggattctcga tgtctcctct tgcgcatcta aattgagaat atttatacca
    10921 tgctctccaa cggtactgct agcagtagat gctatgttag gcatatgcca catcatatcg
    10981 ccaataaggg tcactacacc tcttccatag actatctcta ccttgggata gtatgctctg
    11041 atatggcgct cttgtttttt aacgtagtct gcgtcgagga aaagcaacct acagatttcg
    11101 acgtcatcgt gtctgtacgg agataatttg acgaaatcat ggtactgctt gtccttttca
    11161 agcgaggcga ccaaataaga tgctgcaata ctttcgatcc ttacgatagc gcagtttttc
    11221 ttgccggtaa cgatttttac cagactgttg ttaaagtcat cttgaagcgg cggatttcgt
    11281 tgaattatcg ttgtatctga cgggtttgtc atattcgtaa taactagagg tatatccaga
    11341 ttattgtcaa ggatttcctt tatagcaaca gggtcaagaa tcttcatacc aaacattcca
    11401 gccaattttg cctcattata agaaaggcat tgtatatttt ccaaaccact ctttactatc
    11461 tttggatcgg cactcagcac ggcactatct ttttcaaagt cgaccttcat ctcatagagt
    11521 ccgtgtaaga ggatcgcagt atctgctgct gttctgtcag aaccgccgcg ctcgtatgtt
    11581 gtttcaagac catcaacggt cttacctata aatccgccta agcagacgat ctcgttacgt
    11641 tcgactaaat ccaataaatg actgtcattc cgccttgatt cttcgatcat aaagttggct
    11701 gcttcaaagt tgtcatcagt aattattggc catttttcta tagcaacatg atcggagttt
    11761 atcccatgac ttcgcataat ataatccata agataagaca tagttatctc tccactatat
    11821 gcaagtgttc tcgatctgat aacatcaaca aaacgccggt tttctgccgc ctgctttaat
    11881 gatattatca cttgacggta gaatctgtcg agatgtgcaa ggaattcttg ataatactgg
    11941 ttagaaactt gttccgtggc aattcgttca taaacttccc ttaagacctc aatttctaca
    12001 ggattcgacg aagcatagct cctacctact cttatggcga cgtcagtcat ggaatagagt
    12061 ttttcctgat aagtaataag aggagcagaa aaaacggcga cgacttttgt tttttccttc
    12121 agttgttcta tccgctttat gacttttcca atcaaaatac cgtccactcc taaagcacta
    12181 ccaccgaatt tggcgacagt aacaggaatc aaatatctag ctcgcttcgg aagctgacgg
    12241 cctaatccct atattaaata ttcaaacaat tttccttttt ctataacatt cccaggagta
    12301 acacgaatag atgagccgta tgcactaaat tcccccaaaa aaaccatcta agaaaaaaat
    12361 attcggagcc ttgtttttcc attcattccc cggttcctct tcccgttagt acattcacgg
    12421 tcttgcttta ataagtgacc aatcaatcag gattcagtgc gcgtctcgaa gtttgctcga
    12481 atgattttgc agcattctcg cttagtctta agagttcctt tgcattatct ttcaactgcg
    12541 gcatcaatgc cttagctgct tctatgtttg taaaaataat attgttcgct aatctagttg
    12601 tggcaatcac attgtccgca gtaattcgga ccatgcttac ataaatgtca cttataattg
    12661 gctggagttt ccaataattc ctgatcgttc cgtataggtc tactagactc ggaatccata
    12721 ccgattgcaa agagcttatg agctgtcttt gtgattctat gaacgcgtcc gaaatttctt
    12781 tggtggactc gatggctgtc tcctgaaaat ctttaacgat ttgtgtaaat cgcggaatct
    12841 ctgcttttgc ttcgtttatt ccttggttga tattatcctt gctcccatct aatgctttaa
    12901 tcattgcatg actttgttgg tcaactgtat acttctttcc ttctttttct tccactatag
    12961 tttctaattt tttggcaatc gttgcggcat cttttcttga ttttgcctct tgaggattgg
    13021 tcgtgctgct acttcttgaa tcttgttttt gttcctcctt ttttgttgac atgttcctcg
    13081 attagttcca atagtagata aagattctat tgttttcaga ttacctgcaa tacagtaagc
    13141 aacagaaagt tgttaatctt acatgctcag gcgttaaagt atttatttta ggtggatccg
    13201 tttgtgtaga cattattaat tttggcgcaa ttgcaattgt ttagttcaga gtttggtaaa
    13261 ttgtcatcaa cctcgtccca aaaaccttgc gcttatctca atcgactttt ttcgaatctg
    13321 atacaaaaat gcccaacaaa aggagactag agatgggcct ccttgacgat agcaaaattc
    13381 aagccgattt aggataaggt ataaggaact ctgagcgtga cagaaaagtt ggtcaggaat
    13441 tagacatcgt cttcatcctc gctctccgat aacgtttacg cgtttcatcg catctgtacg
    13501 ccctaaaagc tgagaatcgt atcctcacta gaccattagc gacaatacag tgataaaaat
    13561 aaatttccaa ccaatatact caatataatg acgagtgtta attgaaagta ttagagcaac
    13621 agagtagaaa tgttattcat ggaggtgtag atacctcgcc tgaatttcca acattgaaga
    13681 gtaaactcgt tacgctgaga caacttcgat ataatgacat tccgacaatg ccacgacttg
    13741 tcacagcgca cgttgtccga tatcttgtac acgtacctta tccttatggg atggtagatg
    13801 caagaaggtt cattaacaaa agtcgcagaa acttccagtt aaaaaaggag ctagactttg
    13861 ctatagagct tgtagacaat ggcagcctag tcggcgtgat tagcttgcag aagatcgaca
    13921 cggctagtaa gaatgctcaa atgggctact ggattggaaa caaatattgg aacatgggta
    13981 tcgcgactga atcaataaat ctagtcattc atcatgcgtt ccatgttctt cgattacata
    14041 aagtctatgc gaacgttctt gcaacaaaca gagcatcgat acgtgtgtta gaaaaaaatg
    14101 gtttgaaaaa ggaaggagta ctgaaacatt ccatatataa agatgataaa ttctatgatg
    14161 tagttctata ttacaggctg aaccggaggc tcactaatgc acgtttgcgc agtaagtagc
    14221 atggagtctg tatacctaca tagcagaata ctagtttcgc ggacgtgtaa taatactaat
    14281 gtatcgagtt gatttcgaaa ctgaatatga ttagaatatc ggttttttca tgctagatct
    14341 gcaatttcga cagttcgcac caccaacttc cgcgccgcat gataaacagg agtacttgag
    14401 ttttgtaact tcattgtggt agccaccgct gaaccctagg gatcttacac ctcgcgtccg
    14461 gttgctacga atgtaatgga tgattccaac aaaaatagat atattcagcg agagccctac
    14521 aataagagca tagaagtaga ggaaggataa gctaatcaag aaggttgatc ttacaaacaa
    14581 aaatttacgt cctcatgact tgtcaatcta tatccaaatt gaacagatta atgaaagaag
    14641 tcacttctgc aattgtgaag gagattgaaa actaacatga ctccttatag atccgagata
    14701 attttttttg tagtgactac catccattag ctgtttctcc tattcaacag atgactagcc
    14761 gtttaattat acgtatgtac gtatgatttt tcagtctatc ggaacaatta agtccaactg
    14821 taaatgattt tcttaatcaa tgaataaaat ctgtcataat caaaatcact cacaatctgc
    14881 cgataggatg aacatattgt cggaaatgga acgccaattt gaccatcgta atgcaaaata
    14941 tttctttgat ctgcttacac atacggataa cgtaattagg acaagagcga tttgtatttt
    15001 agcagatgta gcaggcgaag atgctgttga cgatattagt aacgttctca tgaacgataa
    15061 agatccactg gttagacatg aagcagcatt ttccctaggt cagcttggat ttacgaacgc
    15121 aataactgct ctttcaggag cattaagttc agactcaagt ttctttgtga ggcacgaggc
    15181 tgctatcgcc cttggcgtaa taggttctgc aagtgcgaag gaagcgcttg acaaggcatt
    15241 ggacgacggg agcgaagagg tacgagaatc cgcgaatatc gcattagcga atattgacta
    15301 catatcaaag atgaaacgaa ttaataagtt cagcaagatg accggaggat aaattggaat
    15361 aagaggctca cgagtcccta aacatttatt tcgtgagatc ctatcccggc cctataactc
    15421 tgacaactgg agaagcatac taaatatgcg atggagcact ctattagtct tctagtcttc
    15481 tactgacaat aaaataaggg atttgctctt gttggcccgg cctaacctga tgtcgtgttc
    15541 accgagaacg aagcacacac gatagatctt tcctttaatt tccttagttc attcactaac
    15601 gtgattatgg acgaaccatt cggtcgaggc tatgaaaagg atctactgcc agaacgttga
    15661 tcttacatgt tccgatatgg atttgttgat tgcttctcca aagatttcag aagtatttgc
    15721 aaatatccaa tacgaatatc agatataaga gcaataagca aagatctcgt gagttagcat
    15781 tgtcacagta tatctgtttt gatattgtct actacagtaa ttataccgtt gctttttttt
    15841 atcgaaggaa taagatgttc gcgtgagacg tatgttaagt cgttatctgc tggagaacac
    15901 cgttttcaca tgctattttt atctcacgtg ctactctcct tcccatactc ataggttcat
    15961 tgaagattaa accagaatat ggagaaccgt ccaaatagag attggttccc gcaacgattc
    16021 ttgctgaaaa ctcaaatgta ataaactttc cttcacgatc atatattcct tctagacaaa
    16081 atgggcctgt cattccggga ggtacaagac gcctggctgc ttctacgaac ctctctccca
    16141 ttgcgtaaac ttcacctaac aaagactctc tcaggactat cggaatattc ccaacaacgt
    16201 tgtatgatgg ctcaagctct actgctagct gttgcctgca agggattctg ccgataccat
    16261 cgacatccga ttcgtacctt cgatctacgc ccatcaattc aatctcagtt gtgataggag
    16321 aatggaagaa ttgaagatag gccggtacgc cgctgatata ttcctgaata tacaattctt
    16381 cttcgccttt tattgcattg cgcttgatta atttttcaac tccttcgtca aaactcttct
    16441 tatcccaagc caaaaaatag ccgcgaccac ctgcagctcc atgcaatttc acgatgcata
    16501 actcttctat atcatcctta gacttcaggg atcttggtat agctaatcct gagttctcca
    16561 tcagtttctg tttaagttct ctgtccgcct cccacctaaa aatccattta ttaccaaata
    16621 ttggagtaga aatcccttcg atctgctcgc tactcaaata cgcgatgaat gtgccatgag
    16681 gaataattat agaattagat tgccttagac gttgctggca cgtatcatcc aaaatttcat
    16741 taaagttttc gactacaaga atttcatcga tgaatttgaa tctcttgtaa agatttagtc
    16801 tttgcctttc acaaacaagc aatgtttcga aaccttcatc cttggcacct ttaagtacct
    16861 gaagtgaaca atgcgatcca agcgtagcta tcgaataccc cttcttgtct tctgtcatct
    16921 tcttcgccaa atggatataa attaacctag aagagattaa aaatacttac gaaagattct
    16981 ggctctatta tcatacctga cattgattca gaactgattt tttgttagtt agtaaattcc
    17041 ccgagagaga ggccaaggaa taatagcaag caaagaaatg ttacacaacg caatcaatgg
    17101 gccaaacatg ataatataaa gatgtgcatg tgcgcgaaga gtagtagaaa ttaagaaaca
    17161 tggccactca atagattgta atatgctgta ccaacaagcc tggggagctc acgtacccaa
    17221 cagaggaagt ccggggttcg acgagaaatt gtcaggcgct taaacgagcg aattcctagt
    17281 gcttactacc aaaggtaacg aattctgcaa tcagctttat tacaaaagca attccgccca
    17341 cggcggctat accaaggagg actagacgca aatgggtaat gtaatcatcc ttgcttgtct
    17401 tcttagccag tttcatagtc tggatcattt ctacgacctt gcgttgcact aaagaaaaag
    17461 gggaagagaa aatggaaaac gccaaaaata ttcatttgat ttttacttta cgtactattt
    17521 gaacttactg tcaaacttac acgcatccgc cagatcataa tttttcttgc ctctggctat
    17581 aaaaatctga tactctttac ctaataagtg attgctgaac cgcatataca ggatctttct
    17641 cagttctctg catttcgacg aaggtctcgg tgttttgaat tccttcgatt tttccaatga
    17701 gcccaataat gacattgtgc aattcctcca atgtacgtgc gttgacagca acgatcatat
    17761 caaatcgacc agtaacttca gataaggacc taacctctgg gattttcaac aatccagaat
    17821 gaataggatc tttaaatttg ggatctctgt taatgcctac agttgcttta acattaaagc
    17881 cgagcatgcc ttcattgacg ataactgtaa atttttgtat gagattacgt tttgaaaggc
    17941 gttttattct gctataaagc acagatgcat taacattaag ctttttacta agctgaggca
    18001 ccgaaatact tgcatcttta gtaagttctg ataatatcct catatctaac tcgtcaaatc
    18061 tttgcaaatt ttatccgttg tatgtttgga gagtatacat taataaatgt aatttcttgg
    18121 cgggccatta atcttaattc agagatttaa tattaattta ttgtttggac tttagaaaaa
    18181 gtaaagatag caaaaattat ataatttacc acagcgaatc tgaatagatg ttcggaagag
    18241 gcaggataaa gtctggggac tatgtttttg tagtacataa ggaccagaat tataataaac
    18301 tggtcatagg tagagtccaa tcagctaatg gaaagataat tcaagtccga ggcacttaca
    18361 tccgccctac tggtttgatc cagcgcatcg aatctggata tgcaatgggg agacccaagg
    18421 aagtcctcca aaatcctgac cctaacaatt gcattttcat gttgatcgac agggttgaaa
    18481 ccggagactt taatggagac tttgaccaag gcatttgtaa ggctgtatgg ataaacgaaa
    18541 aacgattctt tgttctcgat ggatggataa gagaaaactt gccagatatg tttgcagacg
    18601 tactaagagc tgacacggag caagagagac tggctgcgcg gacaacactt atagaaaaaa
    18661 tgaattcgct ctatgagaaa gatctaaagg atcacttgta tgcggtggtc cgaagtacaa
    18721 aaatattgtg aagttttata ttatggtcat ggacagaaga tgttttgaca atggaatacg
    18781 tatatgctgc cttactatta cacaaattaa aacaagagat aacggaagat aatatcacga
    18841 atgtggtaaa gtcaacaggt gttaccccca acgaggtaca ggtcaaagcc cttgtggcgg
    18901 ccttaaccga ggtaaatatt gatgaagcct taaaggcggc gccagtcgca ttatctagta
    18961 gtccagcagc tagtgctgga ccatccagtt cagcaccatc ttcagcagaa tctaaggtag
    19021 atgaggaaaa gaaggaggaa aagaaggagg aggaggcgct agagggactc tctgcgttat
    19081 ttggttgatc cctttacttt gcattatttg ttttggctaa gatgacgaac tcatccatag
    19141 tggatcgaca ataatgaggc cccgcggctg gattcgctta ttagtattct acttattagt
    19201 attctattga ggaatgtatc cgctcttttt aacctgattt gcaattattt gcccgttagc
    19261 atttgcccta accaaaacaa attggacact gtccggtgac acatatcccg agtcggtggc
    19321 taacgccgtt gcattttgat atgctcttac aagtaagtgt gtgatagtct ctacagtggg
    19381 ataacttgtt tctatcgcca atgaaactgc ctcgtggaaa gatcgggtca actcctccat
    19441 atattcagca aggttgatcc ttagatcatt ttccttcaga accagacctt cagcaatggc
    19501 gaaactaatt gaaattcctg cctcaatcgg tttgatattg agcttgctca ttagcgaggc
    19561 aagcttctga gatataatac tcccagaccg cactactaca gtatccttac ctacccaaat
    19621 agttccctgg tcaattttag ttgacacatt tgcctcctta aactctgaaa gcacaggacc
    19681 aggagagata cctgtgttcc ccgcgggaat tacgatgtct ttagtagcaa tatctccccc
    19741 ttttgcaggc aagaagacct tattcttgtc aaaaatcaaa ttcagcttga aaggactaat
    19801 attcgtaaaa agtagtgcgc actggccctc caattcttta cttaaatagt ctaagccggg
    19861 tcggttgctt agtttttcga atgcacgctg cgcgaccctg ttcttaatta ctctaatctt
    19921 aatttcattt cgaaattttt tgcgaattgt cataagctgt gtagcacgaa ctttggacat
    19981 tttggaaaga gcaatgacat cacatgtccc cggtaattct tgcagctctt gatacagcaa
    20041 ccgttttttc ttaggataat ttttcctttg cgtgtccact tccacttgag acgccatttg
    20101 actattcagt cctctttttg cctttgtctt tttttatcgg ggctaatttc gccgtcttac
    20161 ccatagtaaa ctttaccatg gtgttacgta tgttattctc gccttgggga agctttttct
    20221 ctacagctgc gagaacagca ctagcattag cagtcaattg accatcatct aatttctcgt
    20281 cgccaatttt ggcagatgcg ttaagctgat tctttgctct aatccgaacc gatttacgta
    20341 aacgatctgc aatagcttca ataggagcac cgtacggcat aggggtaggc attttgcctt
    20401 ttggacctag aaattgccca aaagcgcggc cgaccgtggc catcaatgcc gtgtctgcca
    20461 gaaaaaagtc atgctctttc accactttcc gcgcttctct cttgttagtc cccaacctgt
    20521 ctagttcttc tggttgtatt actctatcca cttcagcctt gcgtgctctt gtaccagtat
    20581 caccagtccc gactacacag atgctggctt gtttggttgg tttgtgaggc aaaatcacca
    20641 cttcgtttag gttaaaccct tttttaacat cgatatctct taatacgagt gttagttcca
    20701 cagactggct gaacttacgt gttgttgccc cctctcgtgc ctgcgttacg atcaggcgca
    20761 gatcgctgtc gcttaccata tcacagatac gagcatccat ggataataag ctacttaaaa
    20821 tcgtttagca atggtgcctt tgtatggcgt atactcacac tttttaaatc tcgccttacc
    20881 atattctcat atccttccaa aaaacgggcc cggtgggctt cgatcccacg acttccggct
    20941 ttcttcaagt ttagaaggca ggcgctctat ccatgctgag ctacgagccc ttacgtctaa
    21001 gcctgattcg tagttacagt taaaatcgta ctacaaaaca ggcagcatgg taagttgaaa
    21061 aggtaaaacc acctttataa aatgtttagc cgtcattgac cccaagacat ggcttccaaa
    21121 tctagttatt aataaaatct attcgaaatg gattaagtag cttaatatat caaacatcgt
    21181 cgatttttta atttcccggt tacgaaaagg tataataaca agataacatt acacaatcat
    21241 attgtcacta atttaacgtc ttgtggcagc cggtagaaag tgaatcatat ggttatagag
    21301 gatgaagtta acaataatag aagtagtagt aatcctaata gaaataccca gtcgctagat
    21361 aatgctgatc cccacttttt gcaggaacag atagccagag tattctctat ttggtccgat
    21421 atcagcagat tgcctacggt tggaccctcc ttcgcttatt cccaatattc gagagctgat
    21481 ctcaaggaac ttattgagtt tgggaaaaac cttcttgaat tacaaatcca cttaaacgaa
    21541 tattgggtac aaatcaacaa cacctttgta caagcgttgg ccaagtcctc ggagaaagcg
    21601 cccaaacagt acaactcaaa acaggatttt gaaaattata gaaagattgc tatagatgtt
    21661 tttgaagatg ccttcactgg cttgttcgat tctaaggaat atgcgatctc gtgcggtttg
    21721 gtgctaagca accaatatga tttatttaca catttgcaga ggcttgccga aaacaatttg
    21781 aaagtactca accttcctac ccgaagcgag atggatgatc tgtcgaagga tattcacgat
    21841 ttgaaaaaga ctgttcacga tctgacccgg aaaatcgagg ccttgaagat aaatgaatca
    21901 ggaaacatac cttcctaata cgtccgaaag aacgttaaaa aggggtagtg aaatcaatga
    21961 aaaaatcgat actaatataa tcttaaaaga aattggagac acaacccgta agctcgaaaa
    22021 aactcgaaat gtgcttcata atgcaggcgc tatcgaagtc gggagcaccc ccaatgaaat
    22081 cgtacatgaa gccagactgt acaggcttct acattatcgt ccgatggtaa gcaagacagc
    22141 aaagacgcca attttgtttg tatatgcgtt gataaacagg tcatatatcc tcgatttgca
    22201 acctgataag agctggatcc gcaacttgct cggccaagga tttgatatct atctgatcga
    22261 ctggaaaacg ccggcccggg ttgacaagta cgtctctttt gatgattatg tgaatgagta
    22321 cattgatgat tgtgtagagt atgttagaac aaaaacttca gtaaacaaga taaccttaca
    22381 cggatattgt atgggtgcta ccatgtctgc gatttatagc acacaacatg aagataaaat
    22441 tagaaatcta gccacaatag caccagtaat tgattccaat caagatacta ctgtgctagg
    22501 taatttttct agacatctag atgtagacag aatgtttaat tccattggta atttacctgt
    22561 cgagcagctt tatgcattat tttcgacctt aaagccattc aagcagggag taaacaagta
    22621 tttcaactta gttgaaaaca tcgataatga ggagttcgtt cagaattttc tccgagtaga
    22681 aaagtggctt tatgacacac cacctattgc aggggaaaca tttaggcaat ggattaccga
    22741 catttatcaa agaaaccttc tagtggctaa cgaaatgaag ataggaaatg aaattataga
    22801 tctctcaaag ataaaaattc ctcttcttaa tattgttgct gaagaagatc atctcgtatc
    22861 accacaatgc agtgcgtcgt taaatgatgc agtatcaagt cttgacaaga gattaatgcg
    22921 atttcatact gggcatgtag gacttattgc tagcagctac tctcagaata atgtccttcc
    22981 gaaggtcggt cagtggatta aggcacgatc acaatagccc tctcaatggc ctaaattgag
    23041 aaatttccat gaaatagtaa ccgacaggat gatctactac tagtcacgca gacttacaga
    23101 tctacccagg ttcggtgtcc tttcttctat gaatgggtat tatgattact tgaaccaagt
    23161 caccatcccc aatattcaat gcttgccgtt cagcttcagg gatagagatc ctgcccccac
    23221 tctgcacagt tgtcttgaac atcgcattat aatatgtcaa accttgaagt gtggagaaaa
    23281 aattgtgaat tgcgtcttgc tgaactgccg aataactctt tacaaaatct ctttgtgctt
    23341 cggcgccttt ttcaattgcc tttttaaatg attcatctcc accagcatta tcactcattt
    23401 caacaaaagg ttatacctgc atttatttaa cattaatccc tactaactct ctttgacaag
    23461 aaagtttaaa agagccttat tgaactcatc tggcttttcc acatatgggg tatgtccaca
    23521 atcttttagt attacaatct caccctgagg aacttgtctg taatcttcta agtactgcat
    23581 gggtatcatg ttatcattat ctccccagat aagtaacgtt ggcactaata gtttcggtaa
    23641 tctatcagac agatttggtg agtctcgtat tcccaacaat gttgacatga acgcatactt
    23701 ggcattcggg agcctcattc ggttcacaaa gtctattacg gtgtcttccg taacagcact
    23761 gggatcgtaa gccatgtcca tgaaggctct gagtgcattc tcacgggtcg gataaagtgc
    23821 tgccataata tactggtcaa gaacatgggt agaagattgc attcttcccg ccggggcagc
    23881 caaaaccaat ttatctattc tccggctaaa cctaatcgcg aattccatcg caatccaccc
    23941 tccaaatgat gaccctacca cgctcgcagc cgggatattc aaattatcta aaaatttttg
    24001 caagaacgaa agaaagaaat ccatcgtata ttcgactgtc ggcttgtcac tataaccaaa
    24061 accaataata tccgggacaa ctactctaaa gtgtctagac aatcccggaa ctaccggcaa
    24121 ccatctttca gcagaggccc ctattccgtg caacaaaagt acagctctgg atttatctga
    24181 tacatgcttc acattgtcat agtcaagata tctgatgcta aaaccatcga tacaagtgat
    24241 cttcatggca ctatcgtgtt ctaccaacca gatctacgtc cgaacttgac taatcatact
    24301 tacttattct tttggataaa tacatcgtgc acgaatatct tctgttggag cgactccatt
    24361 tctgaattaa atggagcatt atatcgtcgc gaatattatg tggtggtgga tgtccttatt
    24421 tgtattttat tccatgttga tctgctgttc tgtattcctt gatgacttct tgcatcattc
    24481 gctccatctc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggtgagga tgacatttgt
    24781 gttatctatt cccgactaca aatacaaatg gatttctatc aaaaaggttg tcgtgcgctg
    24841 ccgtcttcgt ttctaagttg tctatctaat tctttaagca atgacttttg aatgtcattc
    24901 agctggactg gaacttttac attgatccta accagctcat cacctttgct gtaactgccg
    24961 tagtgaggta accctttacc tttgagcttt agaatggcat taggctgtgt tcccggagga
    25021 atttttaatt tctcattccc atctagcgtg ggagctcgaa tctcggttcc aagtgcaagg
    25081 tctgtaaagt tcagattaag ggtgtaaagc aaatgaccat tttccaatct ttcgaatagt
    25141 ggatgtggtt taacgtgaat cctaatcaac aagtcgcccg gagatcccgt ttcagacggc
    25201 tcgccctcgc cacgcatttg caaagtcact ccatcttcaa cgcctgcagg aatttctagc
    25261 tttattttct tgttcttcct aaccttacca gagccatcac atatgttgca aggcttgtcg
    25321 atgatttctc cttgaccgcc gcaggtttga cacggctcca aagagacgaa tgtcgaaaat
    25381 ctattttgac tgtagatgcg ccttgtttgc ccttggccat gacatacact gcattttcta
    25441 ggtttggatc caggtgatgc tccacttccg ccgcaagtag tacatctttc gagcttcggt
    25501 aactcaatct cgtccttcct gccctttaat acctcttcta gcgtaagctc gacatcataa
    25561 agtaagtctc ttccctttct tcgtcctccg ccaaagctaa acccaaagcc ggacggatca
    25621 cctgccgcag tcccaaaacc gccaccaccg gcccgacctg cgaatatttg atcaaagatg
    25681 tctctgaatc caccaaatcc catatcttta aagacctcat cgaaattggc ttccgaacca
    25741 cggaatactt cttcggcccc aacgtgacca taggtatcaa atctttttct cttttcctca
    25801 tctgataaaa cagcataggc ctctgaaatt tccttgaact tttcttctga tcccggtgtc
    25861 ttgtttctgt ctggatgata ttgtagtgct agctttctat atgcattctt gatgtcatct
    25921 ttgcttgcat tcttttggac accgagggtc tcataatagt cccgtttgct gctcatggtt
    25981 ggaagtccct aatacctttc atcaatctca gtaacgaaat ggggatacta tgactgaggt
    26041 ggaggtgtcg tgcctgtagc accgccacta gtaccagcgg cctgatccgc accggaagtt
    26101 gatgcctctg ctgcagacgc gtccgcggct ggccctgtgg atcctggagt ggctcctact
    26161 gtcctgtaaa cctctgtgct gacctcacca ataatatttt tcaattcctg ggttttggtc
    26221 ttgatgttat cgatattgtt gctcgaaatt gattctttga gttcttgagc agccttgtta
    26281 attttatcag cctgttctgg tttaatttta tcctttaaat cttgttttac tagtttctct
    26341 gccgcgtaga ccaaattatc agcttcgttt ttaacttcag cctctaccct cttctttttg
    26401 tcagcctcag cgaattgctc ggactctttc ttcagctttt caatctcttc tttcgatagc
    26461 ttggtatttg cagtaatcgt gatctttgct tctttggctg tagccatatc tttagcactg
    26521 acgtttagga taccgttcgc atctatatca aacgtaactt cgatctgcgg tacgcctctt
    26581 ggagcaggtg gtatgcctga gaggttaaac attccgaggg aaacacaatc tgctgccata
    26641 gatctttctc cttgaacaac atggattgtg actgcggtct ggtaatcagc ggccgtagtg
    26701 aataccttgc tcttctttgt agggatagtg gtattacgtt caattaaggg ctctttcaag
    26761 cctcccagaa cttcgacgcc caacgttagt ggtgtaacat ctaccaggag tatgtccgtc
    26821 gctacgtcac ctgcgatgat ggcgccctgg atagccgctc ccagtccaac agcttccatc
    26881 ggatctacac ctcgttcggg ttctttaccc atcactgttg ccacaaattg tctcaccatc
    26941 ggcattctcg taggcccacc gataaggata attttatcga tatcagccgg agccaatttt
    27001 gcgtcttgaa gtgcctgcaa cattggcgac ttgcaccgct ccactattgg tctcaatagc
    27061 tcctccaatt tcgatctact aaggttgagg acaagattct ttggccctgt tacggagtca
    27121 taagcgagaa atggcaaatt gatttcagat gtaactacat ttgacaattc gattttcgct
    27181 ttttctgctg cctctcttat cctcatcatc gccgccttgt cgtttcttat atccagaccg
    27241 gattgttttt tgaattcctg caaaacatag tctacaaggg tattatccat atccgttcca
    27301 ccaagttgcg tatctcccga ggtgctttta acttggaata ctccccctcc catctccata
    27361 agtgtaacat cgagtgttcc accaccgaag tcaaagacta agatctttag gtctttctgt
    27421 aatttgtcta agccgtatgc aagagaagcc gcagtcggtt cgttaataat ccttacaact
    27481 tgcaatcctg ctatttcacc ggcgtccttc gttgcctgtc tctgattgtc gttaaagtat
    27541 gcaggagccg ttatcactgc tttatcaaca gtatccccca gaaaagcttc ggcatctctc
    27601 tttatcttct gcaatatgaa tgaggatatt tgctgtggag tgtagtcttt gccgaatacg
    27661 ttgaatttaa aatcggtccc catctttcgt ttagctgcaa tgacggtgcc ttctggattt
    27721 gaaatcattt gccgtcttgc aggctccccc actatcagct gaccatcgcg ggtaaagcca
    27781 actacggaag gaaaagcttt tcctcctata gaggtccctt cagctgcctg tattattgta
    27841 ggtttaccac ctatcattgc tgcagctgca gagttactag ttccaagatc aattcctata
    27901 atttttccca tttctaatta tcagtcccct tttctgcttt ttctgtatca ttaacggtat
    27961 ttttaactat ctttttagaa atttcgacaa ggctcggtct taaaacttta ttgtctagca
    28021 tatacccttt acgaatttca tctgtcactg tactctctga taaatcgtca cgaaaggaga
    28081 aactgatggc atcatgaacg tttggatcaa atggagtttc gactgtctca atttctatca
    28141 ctccctccga cttgagcaaa tgatcaaagt ttttcaaaat tccctctaag ccttcaacaa
    28201 caactaattc ggctttactt tgcttagcta tttctacagc ccgtacataa tcatcgcgaa
    28261 tatttaggaa tcttagcata agttctgctt tattttcctc tattttcgaa gcaacttgtt
    28321 tgtccatatg tttgcgatag ttatcaaaat ctgccaccat gtacttcagc ttgtttagat
    28381 tgttatccga tgattcctta gctttacgca attcctcctt gacagatgat aattcttcct
    28441 ttgttgcaac tagctcgtcc ttcattgtga ggatgtcatc ccgttctatt cgtgaatcat
    28501 tttgctctac ctgctcgacc tcgttaacct cctcttccaa aaacaatcag caattaaaac
    28561 tcggaataag ctagataatt acatatcgct ttggtaactg aagatttaat actccgtact
    28621 ggatctaatt tttataactt atctaatgaa taagggcaga gaatgtttaa ctgtcccaat
    28681 tgaaaaagaa agggtgtgtt tgagt
//
LOCUS       DAOR01000288            4463 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_115918, whole genome shotgun sequence.
ACCESSION   DAOR01000288 DAOR01000000
VERSION     DAOR01000288.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_115918"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1285 a          833 c          989 g         1356 t
ORIGIN      
        1 agtagcattc attccagcac tcttattcat tgctgcggta gttgcaccaa ttgcgtgctg
       61 aagcgcatat tttgttgcat tagtattacg tgcctgatcg attattgatt tcgttaaacc
      121 aggaggaagg aacgatgaca tggcattgtt tgcagcggtt tgattagcaa cgcctgctgc
      181 gctaggtatg ccggcctggg caatagcctc ttgtagaatt gggcctgcac taaacgacat
      241 tgctccaact atggtgactg ctgataacaa caacaacagg cttgtcactc gttttttgtt
      301 cattattcag agagaatttg tctccaattt atcactttcg accttctatt ctagctgaaa
      361 gtatagttag aatgataata cgagaattac cagatatggt cagagaatag aaacgggctt
      421 cgactcaaac tgagttagca ctattagaag agacatttgt tttataggtg aaaaattaca
      481 tcccaatttc tcaacccttt acttttatga attgaaaaat aaattgaggg atacagcatt
      541 ttcagctgtc ttcctgacta aaccttctat tttctatctg cctatacaga gtccgcttgg
      601 gttactaacc atgcagttgt tgtctgcaaa tgagtttacg ttgatgttcg gagctaatcc
      661 gttggcgtta ttgaggtcga ttacattatg caatacgttg ttcatgttta ttgtgttatc
      721 gctgctttgc gcatcgagga aaagaccatt ttgaccaccc tctatgttgt ttgctgcaat
      781 aattgccttg tgcgtgttga ctaatgtgac acctgcgagc tgattgccgg taattaggtt
      841 ggacataagg cttgcaccct tgctagaatg agatgctact ccgagattgt tgttcttaat
      901 tatgttctcc tgcacttgtg tgttagtcga gccagttgta aagaccgcaa tttcgttgcc
      961 tgttaagatg attgacttaa tttgtacgtt gttagaacct gtggctaata tgccagcctg
     1021 gaaaccctta atgacgccgg gtcccattac catgatgttg tcgttgttgg gtactactac
     1081 tcctacttta gagctctgtt gcccaggacc tgatacagta tagccgttta agtttatgac
     1141 cgtgttgtcg gctcctacaa ttagcccgtc tccggtgcag ttaagattag ctgtcaagtt
     1201 aacgcttcct tggactactt ctccacacgc tgggtttgtt atgggtgtta ttgccgttgt
     1261 tgatgctgac gatgattttg gtgttgatgc tactgatgtt tgtgatggtg tttgttgcgt
     1321 ttgtgtttgt gccattagag ctgatggttg gattaccaaa ccgatcactg cccctaagga
     1381 caagattgtc gctaccatgt atgacattat agttcgtttc attcagatga cggctcgaaa
     1441 ataaactatt tatgcgaacc tactgtttgt acttaattct aaattcgtgt ttattgaata
     1501 taagtatgta tttacgttat tgatcgtaac tatctattag tataagtagt ccttctactg
     1561 aagttccact ctagacttat atttcataac atacttctct cgcatccgcg gtagaatcta
     1621 tctctcggga tattttcgct cctcttagca tattgaatcc attgactgta gattaaagct
     1681 cttgtcgaca aagattaact aagataagaa cggttcttca ctttattgct atcgcgtaac
     1741 ttctctacaa cattattatt gtagtagtac cgatacttgc catattaaag ttcttttcat
     1801 cttaacatat cagacattcc actggggtta ctagtaactg gtaacggaat ccaaaaataa
     1861 gtgaacatga gtatgagtat gagtaacagc aggatatcta agggccccat ttatacgaga
     1921 agaattataa ataaactcac atacttgaaa atcaatttat gaggattgcc gtagttggag
     1981 caggggtctc aggaagttac ttaggacaca tgcttcataa gcgcggagat gacgtagaaa
     2041 tattcgaatc ctcgaggaag gaaaaccagt gggctgtttg tgcttggggt gcgtcgagaa
     2101 acatgctcgc caagttctca aaccgcgcag ggctcgactt tgataattac atcttccatg
     2161 taggtaagac tttgatgatg gatcttccga accacgtcag agaatacttg gatttgagag
     2221 gcttggtaac ttataacaaa cagaaatggg aacacgattt attaaaagat ttgaagatta
     2281 catatggttt caaatgcacg cagcagaatt ttccgttcaa tgagtacgat tatgtgattg
     2341 actgcactgg gatgcatcgc agtttgcttc cgcgatcaga tgaagatttc ctaatacctg
     2401 cgtacgagta tctggtggaa aacgttcaag ggatggatga attttatgtt atcggttata
     2461 agggtgcgag gggatatttt tggtattttc ctcttgacaa taatcgtgga tacgtaggcg
     2521 ctggagatat cgacaagaag tatgcaggag ttgaagaatt tttaagtgaa caccctgaag
     2581 cccgcgttgc aaggaaaata ggaagaccaa tccgccttgc tcctcctaga cgaatggagc
     2641 cattttgtgc cgggaatgtg ataggggtgg gcgaatcgat aggatgtgta ttcccaatat
     2701 taggagaagg aataatcccg tcgctaatat gttgtgatat tttcctcgaa gtattagatg
     2761 gatctaagca gaagggtttt gactttgagc agtacagaaa aaaggtgctc aagaagttcg
     2821 attactatga tgacgtctac agaattgtga gactaaagat gaaaaacaaa ctgaacatgg
     2881 tcagacatct ccccctcatg atgagcatgt atagaaacat gaagaaagaa gagagccgat
     2941 ttggctttga gatcagtctg gacaaaatga cgcggtttat aaaagccttg tagttaaaac
     3001 ttcttgcggc tacaattttc atattatcat aattgactac ttcatatcat agttatacaa
     3061 ataccggtag ataagtggaa gccgttgtct gaaaaggtaa tgtatgccga acttctcccc
     3121 agaaaagcca tgttttgtgg taaatacggt tacatctatt tgtgagtcct actgtacgaa
     3181 gatgcagata cgggtcttgc acaccgcctt tacttctaaa cattcaagaa tactggtgca
     3241 ttatacttat cagaatctgt aatcgtggga caagatgaaa tgagtgtaat cgctcatttt
     3301 tctattctcc agtcaggtaa tttgtcatcg aacaagtgca tgattttcaa tcctacagat
     3361 ggtttcgtta ctgcaaataa atacccactt ttttaactac tgcctacggc ccttgcgaca
     3421 aatttctgtt cttaatacgc cgcacagaaa ctattgtggc caatctgacc aacgccggat
     3481 caataaaata tcgtcgatga cgatcagtgt cttagtaata gccaaaggtc tagtagcgcg
     3541 aataggtagt cccgataaac gtaaagagca aagatcaagg agtctggtac tacatttatg
     3601 tcgactctga atatgactat aaaatcgata tacgccccta tgcggcaatt ttatggtgag
     3661 tggaacaaat ccattatagc cgaagaaagg tcattcgcga caggcaaaga ggggattgag
     3721 gaaagtgaac cccaacgagt tgaaaggacc aatattaata actttaatta atcatcctct
     3781 ggaaattgtc ataaagtggg aaactatttt gcggtggtga cgtgccgaaa ttctgaagag
     3841 actataaaga aaaccctagt ttcgattaag gagcaggcaa ttgccccaga atacgttata
     3901 gtaatagatg acggatcgac tgacaaaaca aaacgtatcc ttgacgacat gaagaaagag
     3961 tgggaaagtc tctatgttat cactagccct gactcgggat atgatattgg tagagtggtg
     4021 aagaactgga atgcggctat caaattgagt agagatagcc ggttgccaaa aactgattac
     4081 catatgatag ccacggatga tactatttat ccaaaatact atgcggagac aataatctcc
     4141 catatggatg cgaaaccagg agctgccatc gtttcaggaa attacgctaa atataggccg
     4201 tttatgcccc acggagcagg gagatttatc agaaactcgt ttttcgaaga gacgtcctgg
     4261 cagggctact atccggagca aatgggctac gagtccgctg ttatatacga ggcaagccgc
     4321 ttgggttatt ctcatagtgt tttggatgat attaaatttg agcatataag acctcttggt
     4381 gaaacacagt ggttttatga atttggagct agtatgcgta ctttgggata tcatcctcta
     4441 tttgtacttg caaagttctt gaa
//
LOCUS       DAOR01000289            6153 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_20987, whole genome shotgun sequence.
ACCESSION   DAOR01000289 DAOR01000000
VERSION     DAOR01000289.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_20987"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    140..403
                     /estimated_length=264
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1815 a         1099 c         1139 g         1836 t
ORIGIN      
        1 gtatcaatca ttgtaaatgg cattaagcca tatcaaaaaa cgacaccatc tggtggaact
       61 aatgactatt caaaatggag ttttatgctt acgcccaaat acaatcctct taaagaagga
      121 caaaataaaa taactgccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacaaaat aaaataactg
      421 ccaaattttc ctgtggtaac gaccctaggt ccgtctcgca taatagcgta aacgtaactg
      481 gcctatctac gaatacaact acttctattg ttaacactaa ccaactgcag aagcatcaaa
      541 atcaacaatc atcaagcaaa aagttcaaga acgaatttca caccagctaa tgctacttct
      601 tattctgggg gtgaaaagaa taacattatt agaggattat cagcttctat tgatgtgggt
      661 aagagtacta ttcatcttgg agataagcag actataactc tcaaggtgag tgatgcaaat
      721 accactaatg ctattgttgg tgccaaagta atagggagaa taatggatgt atctggatca
      781 tccaagaaca gcgcagctcg atggagttac ggatactagt ggtgaagcat catattcatg
      841 gacggtaggt cacaatgatg cagttggcag gtataaagtg gatgtgcaag tatcagcttc
      901 tggttatgga aatgttacag cttcaaagtc atttaaggtc acttctatac cggtaagtag
      961 ctcgagtagc actaacgaga atagtaacaa ctctgttcaa ccaaattcag gcaatagcga
     1021 tactaataac aacaacaata atgataatca taatcaccca tcaactataa tcaaccgcct
     1081 tctctagaaa tggctcgaga catgaaacag ctaatgagat tgcaaaaact ataatagcaa
     1141 tctcgacgaa acaagagtga gcctctgtag gtaatcgtct gttttgcata accacatcta
     1201 catacggggg ctctagagaa ggaatttaac agacatgaga atgtcccaag tccattaatg
     1261 atatctgaca atttcttcct tatggtttgt aggaatctat gggagtcttc attccaaaat
     1321 tatttttgga aagagtcact atgaaggtgg taaggaatac tgtagaaggt gtgagaaaga
     1381 tattgttgaa caaagtctat gattgtattg tatttttaac tttagaaaac ttaatagtta
     1441 atatgtatgt caggcgaaat tgcaaagaaa aattttaata gtagattcgc agtctgcgat
     1501 ttaatgcaag aaaaactcgt tccaacatgg gctctaaagg tagactatat tgaagcctgt
     1561 aattgtgatt ttggttgccc atgtaatttc agtggttttc cttcaaatgg attttgtagc
     1621 gctttgattg gttatcatat aagggaagga aactatggtg aggtaaaatt ggatggtctt
     1681 gatatagtag acgtatattc atggccaagg gctattcacg aaggaaatgg tactcatcaa
     1741 atattcataa caaacaaggc taatcaacaa gaacagaaag atgccatagc cgatatattt
     1801 tcaggtaaag ccaagggaaa tggacatttt gcgctttttg cgtctacggt aaaataccta
     1861 ttggatcctc aatttgtaga tgtgcacatg aatattgatg gcagaaagag tagtttttct
     1921 gttcctggtg ttatggacgt aaagctagag aactttatga atcctgtaac tggaggagaa
     1981 caagacacca aaatacagtt accaaaagga ttcatatgga aactagctga cgcagctaaa
     2041 acgaagatca tgcgcatatc tacaccatca ctgaatttcg atcattcagg taagaatgca
     2101 ttttactctg tagtagattt taaaggtcct tagtagagga ttattctacc actaagaaat
     2161 cctcttaatg acatcaagat atttgctatc ttttttgcag ttttgaggtc tcccaactca
     2221 agagcttttc tgtgaagtgg ttctagtagg agcattaatt cctgagcttg ctcttgcgac
     2281 atttgaccct gttcaaataa acctaataat gtatctaatt tttcgttatc aaaagtgaat
     2341 cctctaagag aactcaatct ttcttctcct cgtccttact aattcttaaa tcacgtctta
     2401 attcagcagc ttcttttttt gactcctcta gatctaatct gaacatatat ctaagatcat
     2461 cttcgacttt tctaagattc tcttctaaat catctatgtc agtatgtgtt tttgatacat
     2521 ctttttctac tcctgttatt tttcttgtaa taacgtacgt ggctcctacc gccgcactca
     2581 gaatactaac tgctatacta atcactaatt gccagtctgt gagtcatttc ttaacttccc
     2641 tgcgtacctt ataagttcat ttctttcggg ctgtccaaac ttttccttac aattcactat
     2701 ggtttgtagg aatctatgtg agagatggta ttccaaaatt atttttggtg agagtcatta
     2761 tgaagatggg aagaaatact gtaaatgata tgaagtttat tattaccacg atggtgtgtt
     2821 ctgtccttgt tgaggtatgg cactgagaaa gtcacttgtt ccatgggatt ccaaaaaatc
     2881 tagatcaacc caaacgagat ttaggcagcc ggcgttagct cataatccat ctgcagacat
     2941 agtaggagga atagagttat caggagtgtg gagtggtgct atagatttta aggtgtttta
     3001 cagataactt caagtaccta ctctgactca aatcgatcaa cgcgtggcaa ggtctttggc
     3061 gggaccaata actataccaa tattaaccag acaatcaata tgaatacggt gactaagaaa
     3121 cagacgtctt caattcatat ttttgatttg gacgaagcga aaccccaatt ctacagtcca
     3181 actggaagca gaacaatagt gaatgcggat aacttcccaa tattagcagg gatgggggct
     3241 gttttgcttc gtctagaaaa aggcggagta cgtgagccac actggcaccc aaatgcggct
     3301 gagttaagtt actgtattgc tggcaatgct aaaatgacta tttttagtac taatgctcgt
     3361 caagatacat ttaccataaa tcctggagag ctaacttttg tccccacagg gtactggcac
     3421 gacatagaaa atattggtaa cgaacaggcg aaatttgtca ttgtatataa tcacgaacgt
     3481 cctgaagact ttggtatatc gggatctgtt ggttctatgt ctgcacgagt tttggacaga
     3541 atgtttgcaa taaatcctcc cggattcttt gatcaattga attatagatc aactcaggat
     3601 atagttgtag gaccaactcc agctgacttt tcaagtagca acaagagtga aactccaaat
     3661 ccacataaac ttaacctggg cggcatcact ccacaaattc agactccagg aggaactggt
     3721 gcattgggta ttgctacctc ttttccaata ctcgctggtt tggcattgtt tctgataggt
     3781 ttgaagccaa gcgggatagt agaacctcac actcatccaa atgcagctga attgaactat
     3841 gttatcaatg gaaaagtacg tttcacagta tatggcccca acgggcaggt tgagacatct
     3901 gaaattggtc agggtcaagt cttttttgtc ccccctggat attttcacta tctagagaat
     3961 ccagataatg taaatggcag caatgttctt tccttctttg gtaatgagaa cccagaattt
     4021 ataggacttg taggaggtct cagcgcatat tctaatgagg tactaggttc tatattcact
     4081 aaagacccaa aattctttag aaatctccca cgagtggaga aaaatgtatt catcgcatca
     4141 ggaacgggat aagcatacaa ggctccgtaa cccctctaca aagttcttct ggtaaccaag
     4201 ggattctgaa atatgcataa acaagatagt tttcaacgag cttaatatat gatagttacc
     4261 catgcaatcg ctaattaaga ccatcattag attggagtta attgggtttg gaaacaattg
     4321 tcaccacaca gttacaatcg catgctatgc agaattcgaa taccacgcta taagtataat
     4381 ctactctaca ggagggttcg caggaatagt ctgtgcgtcg cggcacgtac ctgcatttag
     4441 ggttaatgga ggcaaacctt tatcctaatt acttgcaata attatgcacc tctacacaaa
     4501 tcagatgctg agtgccatat atcgccgtat ttccaaagtg attgctagtc tttgatggat
     4561 caagccatat tcttaccgca cttccatcca caagcgatat acttgttggc acatccttta
     4621 cagggccatt tttcatagtt attgtagatg tgccgttaaa tatagtagaa ttactagtag
     4681 tcgttggtgt tccattcaac ttaaagtctg aaatagtgtg ttgatgcgac gcagtcccat
     4741 ctgtttttat catataaaaa gccgcattaa acacaggaga agtagtattc accttatcca
     4801 tcctaaatac accagaaact atccaagtag gttttaaggt agatgcatca ttttgtatac
     4861 tagtaagagc tccttttatt gaattctttg ataagttagt aggttgttgt gcgtatgaca
     4921 tagttccaat actcaatgca agtgctgctg atattaatgc taaagcaaat gtaagatcag
     4981 tcattcttat tcgcattttt atcattagta gttgcaatat gctttgtata aaaagacatc
     5041 taaagaattg attgttgtta tcgtgaatct tccactacag ggattactga agaataaacc
     5101 cttacctaca aaatttgtcc aagtatagaa ttgcaatgca ctattaaaca aactatcccc
     5161 agaaacgtta atcgttggat cttcagtgtt tccttgctgg ttttgtggca acatctggct
     5221 taaaccgtcg tgttatccac tagcgaatat aatataatga ttatatggta ttacatggca
     5281 tattttcttg agatcatagc tagacttcgg aatgacattg agtttcacgt acggctagaa
     5341 atgaaagaaa gacttccttt gtatttctgc aaattcgctg ggatgccgct ttcatctgac
     5401 atgatcaaat ttgtttcgaa cttttgaaca tagcatagaa gtacttgtag tgtaaagtgg
     5461 actgtctagc agcaatgtct atgaattctc taaagtattt ttgaagttgc tgtcgttgta
     5521 actcagatga tccacctatg acgttatggt tgatgcggct atttttgtgg taggcgaaag
     5581 caatattatt gtcttctctt ttccctatag tcccatgtag aatagtaact gggtgtaatc
     5641 tctaagataa ccgaatcccc atctttaata ctacccataa gagatgtcgc cataggatgt
     5701 gcgaggcttc caaggtatct gaccattatc ttttcagcta tcggtatatt atgattgatg
     5761 tcttcatgga ttttcacttt ccccttgcct cttactccct tatacggtgg attcgacaca
     5821 atagtagagg atattccttg cgttcaaatt ttctatcttc tttgattcct tgtaagtctc
     5881 aatgtaaagc ctattgttag tggcatcaaa gtaataccat gttggatgaa tgtttgggtc
     5941 tcctttattg tcaattgtgc caatgtggat attgagcttg ctattgccca aaaagttctt
     6001 tatttcttcc tcagttaatt gtgtaccaaa tcctggacta gcattcagaa ttctcatatt
     6061 attaaatgta atacggaaac gttcgtatat ttgtgatcat aactggagaa agtctagcgc
     6121 tgccgctcca tgagttcatg gccagttgaa tcg
//
LOCUS       DAOR01000290            4934 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_62593, whole genome shotgun sequence.
ACCESSION   DAOR01000290 DAOR01000000
VERSION     DAOR01000290.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_62593"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1040..1268
                     /estimated_length=229
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1432 a          925 c          836 g         1512 t
ORIGIN      
        1 cgtttggtat tatccaaaac gaccgaaatg ccaagtagat ggatacaacg agttgcttta
       61 catgcgctag cctcttcgaa gaagacaaca gcaacacccc gcggtatgac ttttggagaa
      121 gactgggatt cagtgttacc tggagcttct aaggaagaag ctaagaaaat atttgattta
      181 tttgcgagtg aaggtgcact ctttggatta tcgccaatca ttgttccact tgtccgggaa
      241 ggccctttaa aacccctttc cattttccat tttccaactc gaagaccttg attttcttta
      301 ccgtcggtcc ccatccgacc acggggaata tagatattaa aacgaaactt tttgaaacat
      361 taagacgcac tcgtaactat caaagataca tcaattattt ccacagtaca attatcgcat
      421 tggcaatata ctggttactg gatgtatttc agcccattta tcatgccacg tatccagaac
      481 gtaagagcct gtaactttta catgagtacc gattggaggg atattgatat tttgatgaaa
      541 gttgatacag gcaaaaaatg cgtttggttg tattactgaa tgttgacata ttggttccac
      601 aactaggtca ccaaattggc cttgtatatt cgccgaattt atcagcttag taaactgaga
      661 atctagcttt accctgatat gggaatcacc gtctgtttca cctctgatac tttctactac
      721 acctgaaaca gttatgcagc gattaactac ttgtaatcta tatgattggt aaacatgatt
      781 ccataaggat tgatcacact ttcctgagac tactgcacta gaattattat taggcgtgat
      841 gttttcaaca cctgccggtt catgtttatg tggaaataat aacctattaa aaaagtcaaa
      901 aatagaaaag gcatttgcat tttgaagata tggtattaaa cctaccagta ttatggatac
      961 tattacgaaa agtgtacaaa tgataacttt agccttcact tgtttgaatc acaaggagta
     1021 ggttcttttt gtttttctan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnat tgcttcggta tgtatctaaa tcaagtttgc gatgtggtaa tagcatgtca
     1321 gcaggcaaaa acccacgtct ttagttgagt tgttcctttt tgattgtgtc taaagactat
     1381 attttattgg attttcttcc tttgccaggt agatgtatta tgaatatatt agtcattgaa
     1441 tatatggaca atttctccta cattgctaac acttgctgtc aagcacttga gctactcaag
     1501 tagagcttga gtagcttcaa cagatcaatc cgcaaaatag ctgctcaaac ccattgctgc
     1561 atgacgacct ccgtacaacg gaccgtcaac tgtcacttac agattgccaa aactttggag
     1621 acaatagtgc aggtatacgc agataatctt gaatgaaagt aagataaatc ttctctcaat
     1681 ttttttctga gtagaacgac agaacgtatg ggcctaattc taactggctc caggccggaa
     1741 atttcagtaa gggaaaccgt ctggataata aaataaaaca caacatacga aatacggtca
     1801 ttgattgatg cccttcatag tactagaagg ttcttccttt ttgtattttc cttaagcttt
     1861 caagcaggga tctcagttgc ttcgacgaag gattcgcgtc cttaatttgt ttcatgctta
     1921 acatgacttg ctttaaccct tcatagactt ctttatcctc aatagtgttt acgagtacat
     1981 ctgccccaaa tggtttctgc attaattcta cgacctgctt cagttgttta atggaatcac
     2041 cagagtttgt tccacatacg cagaagcaaa tattatcctc tgttttggac tcattgcgag
     2101 gatttcctct gcgacttgca ttccgtcttt ccgtggcatc atgtaatcca aaataacagc
     2161 atcgaaggca gtatcaatgc tctctcgttt ttctaactct gtctgttttc ctttcaattc
     2221 cgttctatag atttttatgc actcttcccc attcttagcg agtgtaacct catggtttct
     2281 ggcttctaag gcatccttat atgattcaga aacagcaatg tcatcctcag ctatgagtat
     2341 cctcataatg caactattgg tacagtatta ccgaggttgg atattaagac ttgtatatga
     2401 tttcctgccg cgtaatccaa tatagatttg cacataaata ttcaaaagag tgttcatctg
     2461 tgtgggaaac atcaatgtta tgataagatt agtcaatctg tgcatattgt aggctatgca
     2521 tctaagatag ttcgtttgtg tgttatcaat cttgtccgtc ttaccaatct tgactctcta
     2581 aatagttgtt ttatcacaga aagtatagat tcatccttgt ttcgttgact gtactacaat
     2641 ttgcactagc cacgcttcat tttttcctag atctcgcctg tgtcttccac aatgatgcat
     2701 gcctatatct ggcagatgta tgcgtatggt gaatctttac aaagagcgtc ttatctttga
     2761 tagaaggacg aaattgctct ccccaaatct tcttcaaatt ttctcaataa tctatgaaaa
     2821 ctatcttcga gctcctttac ccttttctct gtttctatct taaatgattc aatgtcaata
     2881 gcgccatagg catgatagta acctccttcc ttcagtgtct ttgtttcttt agtacaaatt
     2941 cctaagccta ctaacttctg caaagaccta aaaacagaac ttttatccct atctactttt
     3001 tttgcaagct cttctaaagt catagacttt ttattcttaa ttaaaatgaa tagcagatcc
     3061 aattccaacg atgacaaatc atatagatag ataaaaagat ccttaactgt ggaattcaca
     3121 cgcgtaatag ttttgatcgg agaagttggc aatgcttcca ataattagct tgtgcacatt
     3181 taaaggtatt gcatagtttt ggtgtgtaat gaatggtttt gggtggacat accaaaacct
     3241 attaatatgg tgtcgttctt atccaattta tcgtggcgag attggaaaat cagcggaagg
     3301 tcatatctga tcctgagagg caggaaggct tgctagcaaa tattcttaaa gcactagccg
     3361 acgacaagtc gttagttctt ttcaatacca tcgttattgg aggcggaaat agtgatattc
     3421 ttatcagaag gttaggtcta actaggaaac agtattactc tagaatctct accatgttaa
     3481 aagctggctt gataagcaga aagaacaggc agtaccacct tacgtcattt ggtgatatag
     3541 tctatgaagc acagttgatg attggaaggg cagctaacac aatttggaaa ctaaaggcca
     3601 ttgattctct tgaagaagaa cacaagcttc cagtggatga acgcaacaag ataattaata
     3661 cgttaataga caatcaagat atcataaaga tccttgtaaa acagtcttga actatctatg
     3721 agtataaatg actaaaataa cgcgggctaa gactgataat tcagccgtac agcctgtaga
     3781 tactacaatt agaagaaaac tacatttgtc cgctgaagaa agagatcctt ggcttagaaa
     3841 aaaagtgggt ctagataaat attgaaaata ttgatctccg aagatgacat agatattgca
     3901 ttattctaca aggttgtgtt ggaagccaga aaccatcatg ttgtgagtac tgacaatggc
     3961 gaagattgtc ttactattta tcgacatgaa tttggaaatt ttacttcgcg aatcagatct
     4021 actgccagtg ataacgaaca acaacaagtg ttcgacgttg taatcttaga ttataagatg
     4081 cctaggctaa acggcattga agttgcaaaa gaaatcatca agattaaccc aagacagaga
     4141 ataattattt cttctgccca ttcaagagaa gctctttttc attcaagcaa agagctagaa
     4201 aagcttgtag aacttgtaca aaaacctttc gatcttcata cattaattga cacattagag
     4261 aatagattat tttactccga gttacaaagt ctcaatcaag atgaagctat tgcacaagaa
     4321 tttaatcatg ttacatacaa ataaagtaat gacctctcaa gaaggctgaa cgaaatagat
     4381 aaagttccat aatgactact ccaaaaaatc tcacccactg atagtctcca gatatgctgg
     4441 tacaacttcg gcgattttta cgttatcatc aataagagaa cttcaatgtg aggtttcgtt
     4501 ctgcccttgg ctactaggga ttgtaaaatt gaacgcggcg ccattagtgt agctatcatc
     4561 aatccatatc ttgccaccat gagcttcaac tatacctctg catattgcca gtccaagtcc
     4621 tgaaccgcca tgttttctag taacgcttgt atcgatttga taaaatttat cgaagagctt
     4681 atgtctctcc tctgctgtaa taccaatacc attatcttct actgtaaaaa taaattctga
     4741 gtttgcattc cgccctttct ccaccataat tattattctc ccgcctttgt caggagcaaa
     4801 atcgactgaa ttttttacca aattcgaaag aacttgctca attctttttg gatcgcagaa
     4861 aacactatct tcctttgcag ttctaatatc tgacttgagt tcaatttgct tttcgacagt
     4921 aatcggtgtc aagt
//
LOCUS       DAOR01000291            4811 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_22993, whole genome shotgun sequence.
ACCESSION   DAOR01000291 DAOR01000000
VERSION     DAOR01000291.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_22993"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1505 a          950 c          896 g         1460 t
ORIGIN      
        1 aagaaatgac aaacaaatca gcaaagaaga agaagagcaa aaaggcaggt aaatccacga
       61 aatgacttct ttaccaacta cacaactaag atataaaggt gtgaccaggc cagctataaa
      121 actacattat aagtcagaat tcgcttcatg tcgaaagcgt ctaatttcgc tttgataata
      181 gtagagtttt aatggtccac ctacaaaata tgacttgtca tcttaagtat tgatcttgac
      241 tttaggttgc caggggttag tttgatgccc atgagttaca aaacaaatca taagataata
      301 tctaccatta ttacgactac tttcttgacc atattgataa tgtatccatt tttacaagaa
      361 aaagtacatg catcatcatc cacgactttt gttattgata ataatgcaat ctatcatgat
      421 acagcttttc taagtggtgg tgttctgctt ttgaccatcc atacacagcc aatcgttgtt
      481 catgtaggca attttttccg tatacaggcg atggtcctta ataattctct aggtacgatt
      541 agtttcgctg ctggaccatg tgattcgaca gtgtctgcaa tatttaataa aaatgtggtt
      601 gtgaaacacg aagtaagctg ttttgcagga gcagcatcaa cgtcagcagc aacacatcta
      661 gttaaattga ggccaggaga aaaagtatca ataataggaa cacccatagg gacgacatac
      721 caggccgttg catctggacc gacaatagct agtgtgacat tccattacca gttaggaact
      781 ggggctaacg ctagcatcat aaagtccttt gaatttaaca taatgcgttc atagaacgtt
      841 ctcaccttac atagattctc agcgtagcta gtagatttga gtacggtgtt cataatagat
      901 gaaacgaatg cgattacttc tgaatgctgg taatctcagg ttggatttat catcccagtg
      961 tggatatatc aaaagaaatg gagaaggcac gttaaagtat atgttatgag tctcaaagat
     1021 aagaatccta ccaacatcct tctgcaagag gagatgaatt attactccaa atctacagat
     1081 tctatcatat ttcatatatt ttaatccccg cataacaaat atcttatggt aggaaagttc
     1141 aaggtctccc aggtatctgc gaggtctagc ttaagttccg atattgtctc ctagtataat
     1201 tgtctagacg agggctacac atttcctaat cgcatggttg gcttgctcag tccaacactt
     1261 tggtaccaat cttgcaagac gtaattagca ttgagtcatt tttgcgaagg tatcgccact
     1321 aacttgtatg gccgtgaggg ttgaacgaca gcgggctatt tagtatcaaa tgtataggag
     1381 gatggagttc accaacgtgg cggagcagct atctgcatga ttgatttcaa tcatgataat
     1441 gattaaccga atctatagtt ggctggactg cactctcttt ctttaccact tccattacct
     1501 tctcccacac atgtttctca aactcataag ccattattcc accaacagct acaccagctg
     1561 caagacctat gggtccccca actagaagac ctactgcgcc gggccaaaca agactaccaa
     1621 accaggctcc agacgcaact tccactgcgt aatcatcagg attccctgtt atagagacat
     1681 gtatttcacg taaagatcca gcccacacct ttggagatgt attaactgct acaatacttc
     1741 ttaacggaac gctattaaga aagcccttat attctgagac aattcgtagc tctttttgag
     1801 catctaatac ctgctttatg ttattgtata aattatctaa attagtattg agtcctttaa
     1861 agtgttgtac tttagccacg gtgaaaactc cgttgcagac atatttaaat tattagagaa
     1921 aaaaacatca gtctgtctcg gccaagactg atgtagaatt catacacaaa cacattagac
     1981 taggcgtcat ggttcttgat ggaatcggaa cccaggtacc aggtcctgta ggtgtgattg
     2041 gtataggtgt ccacttctat actagttctg taactgaacg ccacgctaca atttctacta
     2101 attcgtttct agttttttgg tttcgtcctg attcttccgt agtatgataa gggatatgag
     2161 gtcgtcttca ttggtgctac tgtggtcagt atcgtattgc cgataccggt gttattgata
     2221 atgctacagc ttaagaaaac cagattagga tcatttcaat ataataaagc aaacaactag
     2281 gacgatggtt tgtttacgat gaggacaacg gtaaaaacga cgacactcat aataacaata
     2341 acgaactatg aatagcagcc ggtcttatgg agttaagaat aaaatgagtg gcctaaaaat
     2401 atgtatagat tgtggtaaac aatttactcc tgaatcccat gaaaatgatg ttatttgtga
     2461 agattgtcta gtacttttga aaagaagatt ttggagttcg ctaaagatag ggaagaccca
     2521 cgataatata ctgacctaac ataacttgac atatcttctt ttttccaagt aatgttacta
     2581 gctttgacgc gttcgatctc gatgattagt gttacttctg ctagctatac aacaacgctg
     2641 ccttgatcaa tacagcagat aatataccat cgacatcctc tttatgtgac aggcacgcca
     2701 ttatagacat cttttggttt tatagataac taggattgtt cctgtaatgt cacacctcat
     2761 gtttacttag atgaccttat gtttatgctt agtaaggaat tactattctg tctcctaaca
     2821 ttatactacc gaatctcctt ctcattaggt tcccatagcg cacatattcc gtgaggggca
     2881 ataacacctg aacttcgacc attcacgtct tctccctcat cagtaacaac agcgcaatgg
     2941 tgtggtttca taaagtagat gcagttataa cagaaatagc ccgtatgatg ataagaatcg
     3001 ctaaactgat ccatttccat atatctagca gtcacctttg ttaatttctt taacttagtt
     3061 gcttcctctg gtatagtccg agttgaatct tttgtcacgt cggttctaga ttcttaatgc
     3121 acactagata tacgttttat tcctgctaca ttaattcttt agtgagcaat ggcctagtca
     3181 tgaaaagaga acgttgatcg ttcttagttc tttaaggaac tggtctgagc gataagcggt
     3241 tactagaatt attgatccaa aatagaaact gttggaacgc acagacatgt ggtgaggttc
     3301 acgtttagta caatatcgat ggtttcgtcg caatactgtt tcaacttcct ccaagtttta
     3361 ctagaagtag ccaagatcgg ccaagtggtt gacgatttaa ccccaagatt ttctaacttc
     3421 aactgccaaa gaatatgtcc tggaagccaa tagcactttg aaacaaacat gccaacacta
     3481 acattattac tgtgctccta aattggagga aatcaaaagt tatttagacc ttgattttat
     3541 tgcgatgaaa ctatgataat gatcgattac catcagtcag ccattggttc agtttgaacc
     3601 ttgagtaact cttcattctt gatgggtttt cgtagaaaca gatccctatc tagtccacaa
     3661 aatgcctcct accttcctag atgcactcac ttgggatgaa gtgccgtcga atcctctgga
     3721 aaccggattg tccatgtcac gcaaaatatc gagaagtaaa agtgcagtct gtctatggac
     3781 acagcataaa acgcagtttc cttctactta gaccgagcga taacaaacag ttacaatagt
     3841 gatggtgata gacacatgag cagacgacta ttcctaaatg acacttatgg cattacaaaa
     3901 tctacacttg agaagagata gagaaagcaa aaaaagagat gacaagttga taacttcatt
     3961 aaggactgct gtagataatg aaggatcact agataaagaa gcaacatctt acaatgacat
     4021 tttgacgcat atgggctagt attgcaaaat tataattacc tccctaacta gctaacaaat
     4081 tgatacatct ttccaaaaaa atatttatcc ggagcggaac aagaaagaga taagattatt
     4141 caatatgttt atgtttacaa tgtgaagttt ctatgaactt tggatatgat tctttaattg
     4201 gtatatctgc agcattcctg acgactttga gtttccttcc tcaaattgtc aaagcatatc
     4261 gcacaagaag aatgaaggac gtttcaagat atcttatgat tctttttact actggttcga
     4321 ttctttggat aatttatggc ataatccacg atgatcttgt catcgtcggt gcaaacatta
     4381 ctgctgcgac attcaacttg gtccttcttt actatagttt tttgtatgga tcaaattaat
     4441 acctcaaatc tcaacaaaaa gcaaagtaaa acatactttg tgtagtttct ccttaaatgc
     4501 tataaacgaa gctgttaggt tgcttagatc tggacaagtt gatgggagag cactgaaaca
     4561 acttacaaga cgtatctgac tcattttgca tgtggtcatg taactgttct tgccaatggc
     4621 acaacgttta gacaattcac tttaatcgct gacgactata acggtacagg aaaaccgatt
     4681 gtcatttcaa cgagcactta tggcctaggc aaaccattga ctagttcata tcagacatac
     4741 aaacctgtga tatttcatgc ctggacattt aacggcacag ttccaggacc aaccatgaga
     4801 tttacgcaag g
//
LOCUS       DAOR01000292           10384 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_48211, whole genome shotgun sequence.
ACCESSION   DAOR01000292 DAOR01000000
VERSION     DAOR01000292.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_48211"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1324..1474
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1921..2035
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2771 a         2234 c         1975 g         3138 t
ORIGIN      
        1 gaattcgaaa ttcgtctgct cgaccaaaaa tattccggct acagcaaagc atatatcttt
       61 cttccttatc tggtaccata gtataaaacc atgatgaaga ctcagactat agccgtgacg
      121 atcgtagccg cactaaccgt cttgtcaact gggtttgctt acatgccaat ggcgcatgcg
      181 cacggtgttc aggcacagct tcagagtaga tttgttagaa tcctagatga gtcattttcc
      241 gctcagacac tccacaccgg tgatacactt acagtaacag gtactcttca aagtctcgtc
      301 aacacgccgc ttagagcttg gttatcattg ttcagcgatt ccgtaaatgc gggcaacaga
      361 tgggaatttc tagcgaggga tccacccggg aacatcttta caattcaacc acaacaaacg
      421 ataccatatt caatcactgt gcgagcgttg gagccagcaa tataccatgt gcacactcaa
      481 ctcaacattg aacacatagg cccaggtcta ggaccaggac aaactgttca agtcacaggt
      541 cctgccatat tgaaaccagt tccgtggggt aacgttgcct atcaggttat tgtgatcggt
      601 gcgggactag gtgtaacttt cgcaacaaga ccgtggcaag tcatataaga aagctctcaa
      661 ttcctttttt ccttcatttt ctttttcaat attgatgcct cttcaatggc tataattaga
      721 ccaagcttgc tttagtttta gagagggcat atcattcgac tggaatgcag agactcaatt
      781 tttacagcga atttccttca tccctgccat gtattggtga agtacatcag gaacctcgac
      841 ggttccattt gaagtctggt aattttccat aatacatgcc atcgttcttt ccgtagccac
      901 gagagtgctg ttcaaagtat gaacaaatct ggtttgttca ttgatcttat ccctgaatct
      961 tgtgacgagt ctgcgagctt ggaaatctgt gcaatttgag catgagacga tttctctgta
     1021 cgcattctga cttggcatcc atgcctcaat atcgaacgtt ttagaggaag tcttccccag
     1081 atctgcggaa catagtaaga ctgtacgata aggaatcccg agcttctcgt agaattcctc
     1141 agttatttcc aacattcgtt catgctgtag ttctgattct tcaggccgcg agtaaacaaa
     1201 ttgttcaact ttctcaaatt gatgtaccct gaaaatgccc ttcgtgtcct ttccgtgtgc
     1261 accagcttct ttcctgaagc aagggctagt gctagcatag cgcaacggta agtcctttcc
     1321 atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgcaa cggtaagtcc tttccatcta
     1501 agatctcgtt catgtgcatc gaagccatcg aatgttcgga agtccctatc atatacagat
     1561 catcattttc gattttgtaa atcatgtctt caaagtcagc aagtatcact gctccctcca
     1621 ttgcctcttt tcttatcatg aagggcggct gtgcaagagt gtatcctttt tcagctaaaa
     1681 aatcaagcgc atagttaatc aaagccagat tcaactttac tagctcattt tttaaaaaat
     1741 aaaaccttgc acctgctatc tttgcggcgc gttctatatc aagcaatcca agcgagtttg
     1801 ctatatcaac atgatctttg agaaaaaaat cgaaattcgt gattgcacca tgatatttga
     1861 gaatcacgtt gtctttttca tcgctcccaa ttggaactga tttatctata atattaggag
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntataa
     2041 tattaggaag agtcatcatt gacgctctga acttagcgtc tactgctttc ttttcttgct
     2101 ccgtctccaa aagtttggtg ccgatttcct tcatctcttc cctttctgag actgaatctt
     2161 catgagcttt tagcttgttt gatattgccc gcgctagcaa atttttttta tgcttaaatt
     2221 cctcaacttg aacagttaac tccctgcgcc ttttgtccag cgcaatcaaa tcgtctagag
     2281 gaaattcaaa tttccggttt tctaacatac ttctgagaag ctgtggattt tccttcaaga
     2341 gtttcgggtc aagcatgcga gatcactttt tgagctatcg aaatatgctc taatagttca
     2401 tttatagtgt ttagtaacgt ttcaaatcca accttgcagg aaagacaaag aagcaaatta
     2461 ttatcataat cccttggtga catttctatt gatagtccat taggaaagtc agtgttatca
     2521 gctattaatg accttagtac agcatacatt gctttttttt ttgaacctta tttgaataaa
     2581 ggcagatatt tcaggaggag ttggtgtatt tggagtcaag tattgccctc cttacgcaag
     2641 agacaaaatc ttccagtctg ctagacggaa ctttacatct tgcagctgcc ttatgaccgt
     2701 ctcctattcc gccaacagat tctgcgcaat ttctaacaac taaacctaga tccgatcccg
     2761 atttgcaacc gatgcatttc ctacaagaaa atttgtgcat accgtcgctg ctaacggtcc
     2821 acacgaagag cactcgatca ccaagagtgg gtgatccgct cagtacggaa gatacggccc
     2881 caagcatctc ctcagctagc agattctctg cgttgacaaa aatcgatcgt ccactgtcaa
     2941 ttgcgcgcca cttttcagtg agaatcgtag agatagactt cgcaaggatt gctctatacg
     3001 tgtcggcaat ttgttccccc tcagtcagac aggcatacct atcgcccata cagattccaa
     3061 cacctatacc tgcccttcca gttcttccac atgcatttag cagtgtcgaa aattccctag
     3121 cgtcgcgtaa ttgacttctt agatcctcat tgacaagtgt atatgtatac cctacaaggt
     3181 tttccgtcag gttctctgtt ttatattttg aagaagttgc gacaaatttt acaatggcgt
     3241 ccaggatgat acttttctcc tcttgcggaa gctctgcaag tactcgccat ctgccgtttt
     3301 ccatcatcct tactcctgaa ttcttaatca cagtataggc attttccctg ttccaagtta
     3361 taccttcaat ataagggaat gatgtagatg ccagcgcttc atggagtggc ttcgtttctc
     3421 tcccggtaaa tattagatct aagtcgacgg taattagtcc atgagactcg gctgtcttaa
     3481 taatttctga atttatactg ataagcgatt tcttatcgcc ttgatcctgg ttatccgata
     3541 ttgcggatac gacggcaatg ggggaaaggt cccagtttct tttttcaatc tggttcgcaa
     3601 ggagatatgc taatccgccc gaagaaatct cagatccgcc gtcgatgcgg tatctgaatg
     3661 cgtttaggat gtgtgctgag taaggcccag tcagctcttc gtcaggaagt tgatgatggt
     3721 caatgattat ccatctatcg ccaagtgcgt cgttcagcgc agcgcccaag cctgagccaa
     3781 ggtcagatat tatgtaaaag tcatgagctt cggatttcat ttgctctatg acagcagggg
     3841 ccatatcaga gatgggcctt actacgcact tagcacccaa tctcaataag gtcatggaaa
     3901 tgatacttgc agagattaag ccatctgcat caaggtgcgt cataatgcaa gtattactgt
     3961 catcttctgc cacagatctg atcttttcac aaaaaggttt tatagcttct tcaagccctc
     4021 taccgtacat tgtgtggtct tactcgagct gggcaataac tgaggcgtat ttccaatttt
     4081 tgggaatttt ctcattacgc ttataatatt tcgataacct gtgaatcttg gcttcgatca
     4141 attcaagcgc gcggacgttt ctgttatcag tattgtgaga tctcaaatgt ctttgtagtg
     4201 ctagagcctt ctgtaccagt tggtttaaat cttctggcat atcctgtttg attccatttt
     4261 cactccttat ttcagttatg gtcttatcca taatcgtctt cagaagaggt ataccatatt
     4321 cgtctcgcat ccgagtccct atcgcactgg gcgtaaggcc ttctttggat agttgaataa
     4381 ctatggaagt gagctctgta tgactcgacg tgagccatgg cggtgcattt ttagacgttg
     4441 ggcgaactga atgagacttg cctcgagtat gagcatgaat gcgtgccatt tactacgagg
     4501 agatcgaacg tatatcttaa atgttactag ggaaggatgt ccttaacttt gactttagag
     4561 gatacatctt tgataactca agaagatacc aaaaagttaa ataatcgcgc gcgagaaagt
     4621 ttacttcaat atgaataagc caattttgat ggctatggtg gcactagtta ctctaatggg
     4681 cactgcattt gcactaccta cagttcagat agcgaacgca caatatggtg gcgcaactac
     4741 cagcggtggg ggcggcctat ccgtcggagg cggcattgaa caacagctta agctggcaag
     4801 ggagaaagtt tcaaacgctg cgcaagcagg tgcatatggc tctggaacac ccatgcttgg
     4861 gaccacaatt aattcaacgc tactcctcat tatcatcatc actgtaatct ttggcggcgt
     4921 tgcaggcatg ttcttcgtta gagccagaac cccgagaaaa gaggccacga cttaattcct
     4981 ccaaaaatct tttttttatt ttagcgagca gctgccacaa agcagcagat aactgattta
     5041 atgctggttt aatccaaatt cttaattcgg agctgttgcc tgatcatgcc gattcgtaat
     5101 ctcggaggtc atccattttt aatgtcgacc gtagtgagta gccagaacca aaatgtatat
     5161 atcatagaag ccgttttttg tattatgata atgagcgctc ttctaattct tgtaccaatg
     5221 atcaccacag caattaaagg actgcttatg cagattggac cacaatacga cccactattt
     5281 tacgacgtat cgatctacat cttcggcact atgctgttcg ctgtgttttt gttaagcgtc
     5341 attgcgtttt ggctgcgaag aaagggtctt ggcggtggtg ctgctaaaga gaaatggacg
     5401 caggagctgg aaaagtactt cgaacgagaa cagataggtc agattccaga cgaaaaacac
     5461 cattggtgaa atcgcacaac tagttggtgc ccacaaactt cttttatttc attttttaac
     5521 gcattctaga acctatatag gattttctta cggaacttct tggtcctcgc cgcaggcctt
     5581 tcttaaacta attcttagga ttagggaagc tagaggttct gcaacttagc aaatttcgct
     5641 gtcccaaggt tgaaagcgta tccatctgca aataaaatgc gccggacggc cggacaattc
     5701 ctcgcaggca aatcaagttc cgttcgtccc tcttccgcac gcctctctat gtagtttttt
     5761 tactctttag agacctactg ccttacaata gactcggtac atattagtgg tggtctacaa
     5821 agatgtcttt attttttgtt taacccgtta aatgggttta tcactagtga agtctagtct
     5881 agatgtgacc ctaagcaata tttgggtcgg ttcgtcgcga gatcttcatc gtttttaact
     5941 cggtttggag cagcgatcaa ttacctcatc cccaataagg agatactttt gccctgctaa
     6001 aaacgtaaca ctagatggga agatctattc tgcgacactt tgatcatagg aaagcgcgaa
     6061 aaatcttttc cgaatctctt tcgattcgtg tccgtaaagc ctttaacgta cctgaaatac
     6121 gtaacacaac gataggcggc tgcattaaaa caatagtcga caacatggtt ccaagcatct
     6181 ctaatttaca acttattatg acaatgactt ctttttcgtc aatatcaatg aatcttattt
     6241 tggctttttc cgttgctata tgaccaaata gctcggaact tcgtttactg atcgcctcaa
     6301 ctattttacg ccggtcataa ctgtggtcgc cattccaaat agccagatat ctacgtttgt
     6361 cacgccgatt aagcatctac gtaacacttt ttccctagtc ttatttcgat ccattcttca
     6421 agttctcgcc aatatctttc tggtttgata ttacaaagct ttagtaaaga atcaaagcag
     6481 cgaccagata tcatttctct tggacatctt gccccacttg aaagaactaa ttggcaattt
     6541 atcgagttac aaagcttgta cagttctcta atctgttcaa gccattttcc gacactgaac
     6601 ccgtttgcct ggcgtatgtc agcgaagaaa atctccaaac caattcgtgt tcttactttt
     6661 tttttaagaa tagacttcac cctgaaataa tctctaacaa caaaaatgtc caaccaaggc
     6721 gaggaaagtt ccccaatctt gtcggaaact acaagatatg gataattagt gattagggat
     6781 ggcggtctgt tcaaacgata gatcacattt tcatgagatt taccatgata ctcactacct
     6841 atctcatcta tttccaaaat ctcagctgtc ctaagcattt tctcaagatt attatcgtag
     6901 acgcagagat ctcttctcct taacatcaag tcctcattct attcaacgtt gatttgcgag
     6961 gttcatgcgt caatatggat ttgaatttga tcctaaccga gtctccctcc gagagagaaa
     7021 ccttccccat gcagactctt tgtttgtcta atcttaagta cagatttccc ttctcgtcat
     7081 agaaatcaaa gaaatttgaa agtagtgcga catccgtatc ggttagacaa gatttaattt
     7141 tcacgtataa agaatttgcc tcgtttttgc caagaatgga agttaacaga acaattttat
     7201 ttccccaatg gccttctgca aatcctatag aaaaactatc aggggaaaca ctaagtgtgc
     7261 tatttagatg ttccaaaatc ttatctttct cttcagtcgc atgaactata ataccgatct
     7321 cagcagcaga aaaattgcca ctttcattac tgatcatagc cgataagaat ggataatagg
     7381 agttttagct atatcttatc atcagcctct tctgtcgagg acgagagtgt tttttcaatt
     7441 ggtttcgcca tcagcagggc tctagattcc ggggttgaaa tcaattttgt cttcttaatg
     7501 cctatatgcc tcaagggcgc gattatcttc gctgcagcaa ggagatctgc acccatttta
     7561 cccatagttg cctcctgaac aaactggtcg atgctgagct tcggaatctt atcttctagc
     7621 gccttatgag cgatcattct aatctcgtgt tttttggagg aattaagtcg tttttgactt
     7681 agtgcaatca atactactct aaatgtgtag ccatccatcg tcatatagtc gtgcacatga
     7741 gtgatcattg aactcccgcg cctaatcaaa ctcctcaaga attcttttgc atattcatga
     7801 cctttgaaaa tcgtcgttgc actcccctca ttgattttgt ctatctgcac gaatactttt
     7861 gtctgatgtt gtgtgggatc ctgttttaaa atatcgaaca tagtgttttc aatcacgcgc
     7921 ccttttgcaa gatcggcgac tgtaatcggt atgtagttaa gaaaaccgcc tccaaatgcc
     7981 gagggtgatt ttactacaac ccattgcttg tctctccatt tgtctcggac tcgtccacct
     8041 ctcttaccgc ctttgaccat gaaacctaga ctcaaacacc aaaatataag tcatgttcta
     8101 ggcaggctct ctcggctctt tacgacgcag atatgggccg cctcatctgg aacaatactc
     8161 gaagctacgt tcattaaagt agcctatgat tattatcata ttgtcagtta tatctaactc
     8221 tttatctgat ggctgttgaa aactaatctt atgtctaatc attaaatatg acgagtaagc
     8281 actcagaata cttgtgactg ataaaagcat caggtggttc gatgccttca tgggcgtagg
     8341 atacgagttt tatcatgtgc gcgcaaaagt gattttgatc ttcaacaaca gaaaaacact
     8401 ccttaacgca tggcacaaag tcataaagtg gtggccggat gactggataa agatgagatt
     8461 tttggaaaca aattattcat atgaattcat attgtacgga gactccaact ttttagaaaa
     8521 tacatgggta tttttgaaag cccttaatgt gtcccaacac tacatgacat tcaaagctga
     8581 ctatgagggg gcagcctact tgcagctcgc gttatatcga cccaagagag actcctacga
     8641 acttgaatta ttcgactatc ggaagaaagt tactgaagtg ttgttcctaa aggaaccgct
     8701 cgaagaacca caagacggca tcgttctgag atccaggagt ataatacatg gcccgaatca
     8761 aagccctctt tgaagagcaa aacattgatc aatgaactaa tttgggagat atgcctttat
     8821 cagttttatc caggcagccc aatccaagct atagaactgt aatggttgtc ttataggata
     8881 attcgtcaaa gttttggcta tcccgaagtc ataggcccga gtcttatgtt tggcgttgaa
     8941 ttttatcctc aagaagttta ccttacgtag ggtaacacta tcaatgctaa taacagattc
     9001 caaaggtact agcaaactct ttgagtgttt ggatatatcc tcatcgagtt gagcttctga
     9061 ataaccatct atacgggtca ccttggcttg gtcctcacta aagtattttt gttctctttc
     9121 ttttggtgta acaaataccc actcggggat gaatataatt tgcttgttcg tgagtgccag
     9181 aattccttct ttagttttgt tgaaaaagcc tataaagcca agttcatttc cccaaatctt
     9241 tggcaaggtt gctacgatat gttcgtctaa atctatattt accctcataa ccttggctaa
     9301 gaatttgcca gttctgaaat cataatatta aaactacgta ctacatcaga tccggttgaa
     9361 tggatcaaaa aggaggattg gttttgatat tgtttggatt cttattccgt tcctgcagcc
     9421 gattcgtatt cgaaccccat agttcctgcg tgcttcttct cttttagtct tcgataccta
     9481 tacgcacgtt ctgcaaatag tattgctatc gtcattgcga gcgcaccggc ggtggcgatg
     9541 tatattccta tatctgcctc tgccatatca gacttatatt gaagtggttg tatattaatt
     9601 tttaataagt gataagttct gatgttttgc ttaattgggt ctcaccattc ttgtggccta
     9661 tctatctagt ttcgcagagg catatggtaa tgctagtgag tgccgaatat aacatatcga
     9721 tgcaacaatt catcttctct ttatcaaacc tgtcgcagct tgtagtgtag ttctgatgaa
     9781 ttagtgctat gccatcttct tgatgctcct tttaaattga caaaactgac cgaagtaaat
     9841 aatctgcaaa tgtaatctga ctaatcgcgt gagaatttct agcatccact agctggaaaa
     9901 atatacatca aacgcagaaa gatgtccgca cataatcatc ctatgcttat gcttgggtat
     9961 ggtcaaagcg tgaacgatct taggatttta tatccactaa tcgacataaa cttattatgt
    10021 tcttctggat cgcaagagtg ctgagactta aggcgggaca gatgccatcg agaatatcta
    10081 tgtatcaagg aagaaagaaa agatacatct ttgccgctat tgtcgcagta ccagtaatat
    10141 tagtggcgtt ctttgttgca gaacagcaac atccatatag aaattctaag acgtggaatt
    10201 tcgactctta cccagagaat agttctatcc ctaatacatt cgtgcctatt gatacaggcg
    10261 gggtgcaagc aaaaggaacg tgggctgtaa agactgacgc gacggcaccg tctaaaccta
    10321 acgtcctcgc tagactatcg aacgctaaga ctggatcagg atatcatatg ttaattcaag
    10381 cgga
//
LOCUS       DAOR01000293           10676 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_17078, whole genome shotgun sequence.
ACCESSION   DAOR01000293 DAOR01000000
VERSION     DAOR01000293.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_17078"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    797..1086
                     /estimated_length=290
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1224..1540
                     /estimated_length=317
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1724..1843
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4920..5164
                     /estimated_length=245
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2892 a         1863 c         1779 g         3170 t
ORIGIN      
        1 aaaagttttg aaaatattca tcggctgcaa gtgttttact gttgccgctt atgccactaa
       61 ccaataatgt cggtaccact atctaagatt aaatgtttac ttagaacata tgttttttca
      121 tgacgattta ataagcacac aatgaggggt ttgtactgca cattgagtac taccgtatcg
      181 tccttcaaca cttgacagtc tatgaaatcg gtacttatcc ttcgccgcaa tactttggca
      241 ggatcatttt ccatgtccag tatgccaaag gaagtcttca aagatgggaa ggcacgaaat
      301 ccagaggaaa taatatattc taagagataa acagtaaacg gaaaaagttt gattctagca
      361 gtttgacgat ccgagataag tatcactagt agcgatcgta tatcatattg gatatatata
      421 ggaaggtaat tttgaccata tgtccatgat ttgttaagaa gatgcacgaa aagcgatctt
      481 gcagtttttg taatttggat tagatgttcc tctgacattg actagttgta aggttgccca
      541 cttatttcct attctaattt ccttatatga gtctcttgag ttgtctatac tgatggaatg
      601 tagtggacat caggattgct atttgagcgt tagttgaagc gactccagga gaattgctct
      661 gattgatctt ctctagaaca tattggaatt tcgtttgaag catgctccgt cttttggcca
      721 gataccaatt ctttaatacg ctcgaggcaa catgtttgat gagaggtcat ggaatctcaa
      781 tgttacattt gcggatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnncttt tgtcaacaca aaatctgtag ctcttgcatt gttccagcag atgtgactgg
     1141 gaactatagg accacaaaat gcattacatg cgatcgaaga aaaataaaca agattagcgt
     1201 tctgtcagtt ctaaaacgaa atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acatcatttc agcctatttt
     1561 tatagcaacc gctgtcatga ctatcccatt ccttttcatg ttgtttgctt ggcagaaacg
     1621 atcaccaaat agtagtgaat ctacagaggt tgaataatta tctgaaattg gtttactcta
     1681 ataaggaaaa ggacgacaag acacagttga ttgaatacta ggtnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatctatg aacttttcag
     1861 aatcataaaa tatttagatg tatctgttag aatcttttat atttaatccc ttactggaat
     1921 cgcggttctc cacgttgaat agtaacttgg tgttatctcc aagatcacct catttccctt
     1981 ttctatttca gtcaaaagcc attttgcgtt gggatcgtcc agagttccga tgttcttcat
     2041 gataaatttc ttggctatga ggatattgtg actaacatct tcatggattt tgactactcc
     2101 tttacctctg actcctttga atggaggctt gggatcatcg atacaatagg cgatgacatt
     2161 cgtttttctt aagttacgaa ctttcttagt tgtcttaagt gtattaatgt agattttgtt
     2221 agaggtatca tcaaagtaat atgcaagagg aattacattt ggctctcctt tctcatctat
     2281 taaacctatg cgaatcagta agttgttatt attatttgcc ataaaatttt tcgtttcttc
     2341 ttcagtcatt gggtcaccca tgccagggaa agcattaatt attttcatca ttctaaccta
     2401 agagactcgg atatatatct aaatcaacta ttcatattgt ttttttgaag gatagatcct
     2461 tctttttctt ctccttgact tctacgtatt ctttcaatga cttcaaatta tagtcataca
     2521 tatcctcaaa aaatcctacg agctccaagg ttctgcgtct attcaatgaa taaaacctgt
     2581 tttttccttg tttcttttcc gctatcaaat ctgcgtcttt caatatacga caatggggat
     2641 accgtcggca gagcatccga gggatcatcc cagtcctcaa acatgcacca ttcaccaaag
     2701 cggttcggta tggtttttgg ctgatcttag tggaaaacaa gaacgtacgt gcaccatacc
     2761 agcaggtaag tctattctag tcccacttct aacgggaaat tgtgatgccg acggtactac
     2821 tccaggcgct ccagcgagcg agagcgaccc tgctctacgt caatgtgcta tgagaggcga
     2881 tgaatatggt gcaattagtg ccaccctaaa cggaaggaaa ttgcaaaatc tagaccagta
     2941 taggactgca aacggatttt ttaatttgac tgtaccaaaa gataatgctt acaacaatcg
     3001 acctggcaca tggagggcac ttagcgaagg tttctttgta ttcttggagc ctttacctcc
     3061 tggaaatcat gatctccatc tcacaactag cggtcttaac ccgttcaaca cttcctacaa
     3121 ttactctgca gatttaattt accacttaat agctaagcca tgactatctc aatgaacagg
     3181 atgttgctgt ttttgcaata gatacatgcc cgatatggat accaaaaaat cttaccgatc
     3241 caacacataa cttaaccgaa acgcgtaaag agatgactct cccagctctt cctcttttct
     3301 tttattctac tgttactttt tcaataaaga ggagaatact gtacgatact gtgttacgta
     3361 tggatattgt ttgataagca gatttgcata aaattgacta ataatatagt atttttacaa
     3421 tctgtgaacc gaacgttcta ttaggtagaa cttccactat cctcatataa tatgaagcag
     3481 acagacactc atcttcaaca tgtaaatcga aatatacatg caaaaacacc aaacggctgt
     3541 gaagaatgct tacaaattgg ttctgagtgg gtacatttgc gtctatgttt gagctgtggt
     3601 cacgtaggtt gttgtgattc ttctcctaat aagcacgcaa caaaacattt caaaactact
     3661 gaacacccca tcataaaatc ttttgagcca ggtgaaaatt gggaatggtg ttatgtggat
     3721 aagacgtacg tagcataatg caactactcc agatcaactc gttcattttt gctttactag
     3781 tattagtaac tctaagaaag catattgtga atctaggtgt ggaaaatgcg aagtgcagaa
     3841 taggttacaa aatccatgag tagaaggacc tatcattagg cacttttgtt cttaatattt
     3901 cgatgccgac aggttccaga tgtatttttc tacttcctgc agatcgctca agctttggaa
     3961 tctatggtaa tatgacaaac aggatccgtt cctctactat cagcaaaaat atttgaaaat
     4021 agatagaaat tgatggatat taggcatcat acttctcttg ttcatcaata gtaactttca
     4081 acgacacttc tgcaccattg aattcttcca cctgtgattt tggtaaatta tattctttgc
     4141 ggtcgccttc tgtcgtaatt acaactgtat ttccttctat agcatttacg tttcctgcat
     4201 cttgcccatc cttagaccta acattctttt ggataatcac atcccaacca gatacaacat
     4261 ctagtgacat ttttcttccc agactatctt ttgtacgcta tactattttt caaccctgat
     4321 cgctatggct agtttcgatc ttattccttt tgttcgcatt taggatgcac gcaattactg
     4381 tatactatca tttctgggta tagcccagat cttctataaa tatggcattg tcagtactgt
     4441 tgctaactgc atctttcttt tagtccataa tattttagct ttaggagtag gagtaggaat
     4501 aagagaagaa aaagaagata atgatgcgat cgacaaaaac tgaaggcttt tacagggtgt
     4561 ctaatgctta ttggattatg gattccgttt ccaattagtt agggttttcc tgaatccact
     4621 tatttggtaa ctaaaacttt cgatagctga ttatatgata tatttctcat tctcctccag
     4681 cacttgtgga gacgaaagca catcccctcc gggctcggat acgtctattc agaagtatac
     4741 tataactaga tagcctaata aggtcaaagt gcgtatattt agtcgtatac tacccaatat
     4801 cctgatgtct tggcagtagc gatgcgtatt cttaagccat tatatttgct atctgttttt
     4861 gtcatggggg gattatgctg tagaaataaa aaaaggagaa tgaaataata aaatggtctn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnatgaaa taataaaatg gtctgtaagg gaatatgcgt acgtcataag gcaatgaggc
     5221 cagttggcgt tgctcgatat tctagtgggc agaaacgatg ccaaatatgt gagatcttct
     5281 tagaatggga cgggttatgg tgcccatgct gtggttatag attaaggact aggccacgaa
     5341 actcgaaata taagactgaa ttgcgagcaa gaaatgaaat agataaaagt aaatcgttag
     5401 ctgtgtacga gcagccgcct ttccaaggta atcaaaaaca tatagctcac aaaagaaaac
     5461 attaagtgtg ttaaggtcga agttatcacc taataaatga atatccttag cagtgatagg
     5521 aatgtaccga tggcagtatt gcaacaagat ctttgatcct taaccttgga ttagttcaac
     5581 aatacagatc gcaggttttc ttattgcagg aattatttca ttatctcttt tcatattggt
     5641 ggaaaaagga tcaatatcgc cactggttga ctttggcctg aagttgaaca agagaatgcc
     5701 aaattaattt tttcttgact aacgtcacat gttgttctac gtatcataaa tacagatgga
     5761 tcacgaataa gtgaatcttc tattaagtta tgcatttcat tatagacaga attgatactt
     5821 ttagagtcga gaaagacaat cagcatactc ttttataagc agcatccttc ccagtttctt
     5881 ctaaatatga atatctaata acaataacaa gaatagctac tacaacatga ggagtagcag
     5941 acaatgaaaa tgggcacacg atgtcctatt gtaagtggtt tcctcagcta gtctattatt
     6001 ctatcactgc gaccaattta cctttcgaca ggttttcctc catgtaacga tgcgcgtcta
     6061 caatctcgtc aaagtggaag actttatcta ggttcacatg gtagcgtccc ttagctacac
     6121 cgtccacaat acgctccaaa gcttttgtgc tactagcagc tgttatagtt tcacttttat
     6181 acacagtgag tttgactgtg gaaggtatag ctaatattgg ctcgaaattt tttattgtcc
     6241 attcgtttcc gagaatacca gtattacaga ctattccttt cggtgcagca gcgtgtaatg
     6301 agtcaagcag cgttactgtt ccaatcaact ccagaacaca attaacgcca ccaccactat
     6361 caggaaaaag ctgttttact tctgaagcga tctggccatt gtccataatg acataatcgg
     6421 tgccgttatt gtttaatgca tctattttgt tcttgtttcg agtagtagca acaatggtta
     6481 gacccagatc cttggcaata gagatggctg ccattcccac ggaagaagta ccaccacgga
     6541 ctaacagagt ctgcccagat tttacatcca ttgcttcaac tagcgatccc caagcagtca
     6601 ggaaggtctc tggaattgca gccagagttt cccaaggaag agtcgaaaca ttattcttca
     6661 atggaataac ctgcttagct ggaactagtg tatactctgc gtagctacca tcgtattggc
     6721 gccccattcc acccattagc gctgtaacag tctgaccaac tataaaacct gtatctggtg
     6781 gcgcagcttc aaccatgccg acacactcta tcccaagtat acgagggaat ttgacatcag
     6841 gagaatgtcc ctgtcgtgtg aacatctccg aacggtttag cccaaatgct ttcactcgga
     6901 ttaagatcca acctggacgc aattctgggg ttggaactgt acgaacttgc agaacttcag
     6961 gaccacctgc cctctcaaca acaattcctt tcataattta tttcagtcct gtatagtata
     7021 gtatgagggt actgtaactt attaaatggt aatgatccct aatttacggt ttaccgtcta
     7081 agcaattaag catacctatt aaataatagt tcacctagat tccattttaa ataaaatggc
     7141 gatttagtaa aatgactatt gaaatttgta ccatccttga gtaattcttc atttctttga
     7201 atctcaatgc gttctaatct atcaatgaag gtagtaccgt ctaaagccat atacaaatca
     7261 tagctaatta tatggtcttt attatgatgt agaataagta tgaatggatt gaattcctga
     7321 tgcaattggc gttttccaac atgctttgac ctctggagat cgctgagcac ttcgaaaatt
     7381 atcccaaagt atgaaatagc attcttaaga aatgctttca attctgatat ctctttttga
     7441 tatataccat aagaatcatc attccattct agataataat tgccaatctc tatcttaggt
     7501 ttttcataat tcttaggtat aaggtgttct tttactacgg gttctggaat ttccaagaat
     7561 ttcgtaatct tctatcaagt acataaacgc tgggtttaga agtgtatatc tgcttgtttc
     7621 tttcaaattt tggactggtt ccaaaaccca gttttatttg tccacgatgt cacgtaaata
     7681 ctccttccag acgcgggcta tccttctacg ttctggtcta ttttgctcac gggaatcatc
     7741 tttccaaatg ttttaatcca ttcatacttt taacaaagaa atcattccgg tgacgtgtat
     7801 cattgttatt ctgtctgtct gtctctgtta tactgacgtt cttactacct gacgttcctg
     7861 aataagagta agagttaaca accatcatgt caagtagctt cataaatgat atatttgatg
     7921 gtattactcg ctttgccaga agacatttct cattctcacg cctcataact atgagttcac
     7981 tatcgatgcg cgcaaagaaa gcaagtaaac aagttgaagc taagtagtaa ctctaagcta
     8041 cttattatca taacagtagc aataatagcc atactggcat tcctatcagg caatattaag
     8101 tatatgatca caatagacgg atctgactct gaatatatcg ataactccat ttgctttgga
     8161 aatattattt cctgctaatc ttgctcttct tgcttattca ctaatgtttg tactctagtg
     8221 tccgtcgaat attgcggagt atgtcaagat ttctaattat tatctacttt aggggtgctc
     8281 gtttttccac acatgaattc tgtactgtaa caattatgag tctatcaaca gaatccgcca
     8341 tttcaaaaat tctaatcaga aacctgtcac gtttacacta ttaaatttcg tcagattagt
     8401 aggatttgcg gaacaagata tttttgctgt tattttattt tgtccgtgct ttatagcagc
     8461 atagttagga gtaatagcat accgccagct agaatagtcc gttgtcccac tatggcctgt
     8521 cgctagcgcc ttctgatacg gttttgcacc attaagtaga attgaaactg tacaaccagt
     8581 attcgtctta tctactgctg gcggtgatgc tatccctgat accacgatat tgccactaac
     8641 tggaagctgc tgacctttat gaggagaggt aatttttaca gagggtagat tttttgctgg
     8701 taagggtgat gatgatagag cagatgaaac accggtgagg gaggatgcgg tcaagagaaa
     8761 tatggctgtt agtccagcaa atgcaaccaa gcgtacgagt tggccgaaat tctttattct
     8821 cgaatattct tccattttct aagattgtat catgttacct ttaaattata tcgcaatata
     8881 cattagataa catactaata ttattatgac aaggcatcag aggctctcgc ggctgaatta
     8941 taacacataa attctggggc cttagtaccg atacatatta tcaatataga ctccagacta
     9001 tctggatctt ctgggatttg gcgaatatgc ctttttttta tctattgaat ctatctgaat
     9061 aactactctg ttgaattgat gaagttgcta catgctcgtc agtttggata tttcgaaata
     9121 aggattctag tcaatgaatt atggagaatg ctattaaata aatatgccat cgggtatgtt
     9181 aatctgattt atttcatcct gtcctctgag gttatatctt aaaaccttcg ttttcaaaga
     9241 gctctggtct gaaaattcga gttttgagat atttaatcgt atagcactag aatcaaacaa
     9301 ttgacttgta ttttctttta tcaacgattg taaaacaaaa aaagactagg gtagtatttt
     9361 tgtaacgagc gggacacatc tcaagatttt gatcattact tttatagaac tgtgtatgaa
     9421 atgtgatagt tcattggtga aagggttaca atctattagc atgattctcc cctttttgct
     9481 tctgatatcg tccgtgattg ccctagctat ctacttatct ccgaatagca ctcttggaca
     9541 gtctagcaca agcggtactg aattctggtt agataaggaa aatactctaa gaatggtatt
     9601 tagctataca ccagcaatac caattattga cacacctact gacctcaaat ttagtattca
     9661 aaatttgcaa actggtaaac ctctaaccga tgtatctgca agagttgtta tactaaccaa
     9721 tagtagtggt caggagagaa tctttagatt cactaatata atcgcgcctg atggcgtctt
     9781 ctccatcaag tatctgtttc ctgatacggg attatatcaa gtcataacga ggataacctc
     9841 acaattgaat ggtgttgcat cattagcgtc attcaaagtt tttgttccag gtcaaccaaa
     9901 cgctttttct ctgaataatt ctagcgggag ttccaatcct tggattatct ttgttattgc
     9961 tatcgcagta gtgatcgcta ccttcttaat cgtaatcaat aggatgaaag cacggaaatg
    10021 gaatatatga cgctatcccc agtcgatatc actatccagc agtgatacga taaatttaca
    10081 ccacgtggat aattgaaaat tgtacgaccc agatatatct agattggttg caatgctact
    10141 acttttcttt tgtaacagca aaatcaaact tttactgaca ttttgaagtt atacacaaca
    10201 ggttcgactg gttgaaacag gatggcgtca atagttactt taggtacaat gatacctggt
    10261 gtgtcaaatt tgtaagaagc atattcaact ccccatgatg aatgaggtgc ctgttgatat
    10321 aatgtcttat tctctgtatt gttaattgaa aagacatagt ctatatgtag ctgatttttg
    10381 tcggtgtttt tgtcaataaa gataatttta aaatgggtga tttctccgac cctcggtaca
    10441 gctgggagtc catgttagtt gtacatctac gttgttcttc ggattagtta ctgttactga
    10501 agagatattg tctgtaacgg aaacatcacc tcgcatccaa tgatagagag gagacgaaaa
    10561 atagtaaatg ccaatattgt taaacttgaa tacgaaggaa ccatttgatt ttataaaacc
    10621 agaatcagac aacaacgctt ttgcatcaga tgtagatatg actatcctat gattta
//
LOCUS       DAOR01000294            8370 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_13997, whole genome shotgun sequence.
ACCESSION   DAOR01000294 DAOR01000000
VERSION     DAOR01000294.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_13997"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2678 a         1502 c         1505 g         2685 t
ORIGIN      
        1 tatctatgta agtactacaa ctaactaagt ggctataaaa gttcagatta ggaatgtttt
       61 gacctagtgc tacgcatagt tgaccagtgc tatgaaatcc tattgatctc aataatatcc
      121 aaaacatgcc agacattttg tctacctcca aattcctaaa tatatccttt gcaactagag
      181 atttactgct agattacagg ttatgcctca cactgactct ctattatcta gagaatggtt
      241 gaaaatgttg aactacaaat gtattagatc ataattcggc taaacttcgc taatctgatc
      301 aaaaaaggaa tctcttaaat tattttctct taattttgct tttgtcttac tttcttaatt
      361 ctttttttag cttgtttctt gctgctttta tcctcgggag tggttagaga tgtgaatatg
      421 cgatattcaa ggctaaaata aaagaaagtt cattagttgt caatgggtag gtcattatta
      481 tccttgagca tatttccaaa aggaagacac tgaagaagct aacccgtata aaactataag
      541 gtaacccaga ctgcgaggtg cagtcgctga cacttctcgg cgaagtgtat ctattatttt
      601 atcttattgg aagaaaaagt attccctggt gagcttttgt ctgtaagcat agcaatttta
      661 gagttcataa tcacgttatt atgaacattg ttattagaag aaccaggtgt agcatcaatt
      721 ccatttacac tatttgaaat ggtgttgtta tagacttgat tgttatgaga ctgagaaata
      781 tgtatcccat taatttcatt gtagatgaag ttatttctga caatagagtt atacgtattt
      841 ctactaagca ttatgccaga cttctcatta tgatatgttc tattgttgtc aaacaaaata
      901 ttggagcaat ttactgaaca tattattcct tcttgaccat tatcgtgtgc cagattattt
      961 cggataatta tatcatggct tcctgtgtgt ggatcgaatc cgtaattgag attgtggtga
     1021 ctttgattct tttctattat catattgcct accccactaa gatatggacc ataccacaaa
     1081 ttgtgaatat tattgcctct tagtatgcta ccgtcacctg catagtagtt aagaccccca
     1141 gcaccaagac cagcaaagga agtgtcgaat ccaagataag caatctcaga attcacaatg
     1201 tttgtagtac cagtagctcc aggttctatt ctgagatatg gcctagggat tgttgcatta
     1261 gatgtcgtta atgtgacata ggtattagtc aatggattcc acgatgtgat ttttaccgaa
     1321 tcaatattca agctaccaaa tacatgtatg gaatttacat tgctactacc agaggtggct
     1381 ccccttcgat tattgtggga acccatccca gcggaagaag atgaaattat ctttagccac
     1441 ttggtatctg tagaattaat atggaattta gaacctttgt caattataag atttgcattc
     1501 aacaaccacg atgcactatc tatagcttgt ttggccagca cgttgctgtc atggagtttg
     1561 ttattgatgt ctgtaaggct agctgaacta caacttatca ttatactcct ggtagatgaa
     1621 tcataggtga tgcaaccaag catgtctgac tgagcaggag agggagttgg attgccgcct
     1681 ttcgtggtag tctgagggtt atgagatgaa gaagctgatt tgtgtttgat ttggttaatg
     1741 gcacttatat cttgtatcgt aagtggcatg ttatttgtag ataaaaacat agggagtaga
     1801 aaggctaatg taaagcagat agtgataaga gtatatatca agatagctat cgaaaattga
     1861 ccgcacaatg gatactataa aaggccttga gtagatatat aatctttgag aaaccgaagc
     1921 cgggtaccta cgtggtgtta ttattagagg gaagtaaggc gcgaagtctc cctaattcga
     1981 tagatcctta gaaacccata tcgtgtcgaa tctgaatagt aatattttct gagtcagact
     2041 caagaatttc ctacgactca ttttcacgtc aagccgtggc ataattctgt attcctgttg
     2101 taacaccatc aaagggtact ttaatagtct attgtgtaac ttttttcctg atgcactata
     2161 tatgagctaa gagtagatat ccgactttaa ttaacaattt taatctatgt ccgggcccct
     2221 gtttagatag gaaatttata taatgcatat aaattacaga ttattaaaaa tgagttaatg
     2281 cgaacaggtg gattgaatga cattttgaaa gctttgttag ataataataa ctagtgtatt
     2341 tcaaatcaag agttaaaatg gttactccag aagattgaaa atgacgcaac agaaataggt
     2401 catgctaaat atggtttaga cgaaaagatt aagttcaata ttgaatgtga taacgatccg
     2461 caggataatc ttataggccg acaaagccta aagtcacttg aatgtatcag acaatctatc
     2521 aagaagaatt tggagttaat gaacgaaaat ataaaaacaa tttttcaatc gtatttaaca
     2581 caatatggag aatgaatccc ctgctttgtt tgcgagaaag attgtagtta taacaaggca
     2641 atttttagaa gaacatcagc aagaaaacaa gtagttagta actctagaca gagaaaaatc
     2701 ccatacttgg gagaaaaaag aggtctgtat agtgtgcgaa agggtaactg gggataggac
     2761 aaaagacata ctgaaatttc atggaagcaa tgaaatggct tccttgaaga tccgagtgcc
     2821 aatataccca aatcagctct aagaggaaga actaattagg attaagaata caaagaacta
     2881 aaaagcaaaa ctagaaaaac aagatataaa taaaattctt gaaggtgtta aacgaaagcc
     2941 acaaaactgg aacgattttg gggtaacgat gagtgtgtct actgacgtct tatcgaaatt
     3001 tcagagccgc atgtgaacgg cggttggaga aactagaaaa tattgaattg aaatgcggga
     3061 gccttggctc agtccatgcc cttagatctg ccgatataga agtagactct tttctttgtc
     3121 agatgatagg aactcttgat tctctcctct ttaagattaa tgataaattt cgattaggca
     3181 ttccaattga tcgagtcgaa atagacaatg tacagagtgg tctatctgca tagactacaa
     3241 gaatcgaatt gttgtctgat ttagataggg catctcgaca tggaaattgg tattggatga
     3301 taaagcaact aaaaaattat tcgttgcacg attcttttgc tgggcacaga ttcttgaatt
     3361 agcacccggt tttaagaccg gaataaaaat aattccttat tttgagcagt ctattaacca
     3421 actcatgaaa ttaatcgaca atactaaaat gaaagaatac cgaccacata atagtaacaa
     3481 gacatgaggt aagagggcaa agcaaaagtg gacacttttc agaacttccc aatcaggtca
     3541 tctcttcttt ttcgtttcat tccgatatcg tcaacgaggt cttgtaatga atgcaaacac
     3601 tcttttttgt tgcttctggt acagttgtct atatgtcaat cttatcttgt cttgtaatga
     3661 tatagacata tgctatgact gattcttagt caacaattca taacagtata ttatacccat
     3721 acccatcttt tctttttgga tgtggaaaga aaaataatta aatatcctgc cagagtaaaa
     3781 ttgccgtttc tagcattaat attgattaca cttattatca tgattgtagc ttctacagaa
     3841 ttatctgcgg cttacataca gcgcgtatat tcgattagaa ctactgcaat ctcctctccg
     3901 tcttcttgct atacttataa tccaactgct agagtaatta ctgtatcttg tggttctgcg
     3961 actctgaccg acatttacaa ccatctccac ggtaacaata tcctaagcaa acaatctcca
     4021 gatggcgtat ggctcttgaa tgcaaagcta gtaattgcta aaggtgctac cctcaatatt
     4081 gattcgtcag atactaaatg gctaaagata agctcctcag ggggaactac tggtagtagt
     4141 gcttctcctc ttccaaatag catagacgtg catggtactt taaagattga ttcagtaaag
     4201 ataacgtcat ggaattcgct cacaaataac tatacgatca ctaatggaac gagacatgat
     4261 ctaggagttg gcaaaagtga agcaactgta tctggtgcac ctagaccatt tattacagtt
     4321 gaaaaggatg caacaggaac tactgatatt ataaattccg aaatagcgta tttgggatat
     4381 gaaaaaggca gtttttctgg tgtaggtact gccggtctta actactatgg gggtgatgga
     4441 agcattctac gaggcaataa tatacatcat ctctattttg gcttctattc tagtggggtc
     4501 ggaaggatgg ttatcgaaaa caatcaggta catcataaca caatttatgg tctggatcca
     4561 catacagcta cacatgatat gattattcgt aagaatgtag ttcacgacaa cggacaattt
     4621 ggcataatat gttcattaaa ttgtaacaat attactattg agaataacga aatatatcat
     4681 gatggtaata aaaatactgg agctggcata atgtttagta gaaacatggt taattctatt
     4741 gctagaaaca atattgtcca tgatgaagtc tccacagcca tttcaatttc tgaatcacat
     4801 aacaatctga tattcaataa cacaatttca aatagcgggg gtggaattaa tttaaaaaat
     4861 tctactagta ataagattta tgataacact atcataaatt ctaaaaaggg cattgattct
     4921 agtgccgctg gtggagtagc tggtaacatg atctataata ataactttat taatacaccg
     4981 tcatcttctt cttcttcacc tcattcatcc aatagtgtcg gttccaacaa tcaaccacaa
     5041 ggaacactaa aacaccacaa taaacattct ctaggcggtc acaaagccaa acccggattt
     5101 gcatgatcag tcagctattc aattggaatt ggttttcttc atatacaaac gaaataaaag
     5161 ttactgaatc atcttctgat caccaattac ttaattttct acacctcgtt attgcataaa
     5221 tctaaaagaa tgtagacggc ttagacagaa agagagggat atgggatatg aagggtagta
     5281 tgaaccacac aatactctag caactactcc caacctctca tcttaaccta aatctacacc
     5341 taaattattg ttgaataaga ctatgggata aataaacaca ctactttcag gagttgctgc
     5401 gaagcactgc agatataata gactggaaga aatgctctct tgcaagaata aatcacaaca
     5461 aagatgactt tagcaatgat catttctcga ttgtcaaatc gaaaaattga aatctcttcc
     5521 cgaaacatgt gaatgcagtt taatatgagc tagttgtaat ctgagccatc tattgtcaga
     5581 catattgctg catttgtcac cgttattgat gcaaatgttg gatctgtttg ggactatggc
     5641 tttcgcagta accggagctt ttaaagcaat tgaacataag gcagacatcg taggcgtaat
     5701 agttctctcc acaattactg gagttgctgg aggagtaatc cgggatataa tctttggaat
     5761 attcccccct actgtcatta tcaacccgtg gtatcttata gtgacaatag ttagtggaat
     5821 tgttattttc ttcctttatc cgactctcag gggacattgg aatttgtttt tgaaatttga
     5881 cgcagttggc ttaggtgttt tcagtatcat tggagctacc attgcttaca atctcttcgg
     5941 gttgaacttt cttgttatgg ctttcgcagg aacgctgacg gcagttggcg gagggatact
     6001 gcgtgatgtc tttgtaaatg aagtaccaat tgttttcgtt aaagagcttt acgcttctgc
     6061 gagtttcgta ggaatagtga ccttctttgt tcttttgttt ataggggtta acgtgaacat
     6121 tgctacaatt ccctctatac tgattgtaat actaatccgt cttgtatcaa taaggtataa
     6181 ttggaattta cccagagcaa aaatgtaacg tgaagctcaa gaggaacaaa ttttcaaaga
     6241 aagatttaac accactgaaa tgaaaattat ttctccactc ttaggtttac catgctgggc
     6301 gtatgcactt tttaactcat aatatagttt agaatcatag tcttgatttg atttggatta
     6361 tttaattgga tcaaggattc atattacaaa atttacagaa tatagctagc tagtaccgcg
     6421 aatcctaatc ctgcgaacat cactacaaat tacgtgaaga ataaataaag atggaatcga
     6481 atctgtcaga catactgctt tattattcta ctttatttcc ttcctttatt attacgtttc
     6541 aatttccttt ttttcttaaa tactatttcg agtataccat ttttgtatgt agatttaact
     6601 gtttcaatat ctgcgtctgg aggtatatca ataacttgat attttctttg tggataatca
     6661 gtagatactt ctaccgaatt attgtatgcc tgtatcttga tatgttcttt gctcaaacct
     6721 ggcatttcta ctacaacttt gacttcttta tcatagacat taatatctgc taagggctct
     6781 ctttcagaca ttatttctgt tctgccataa ttggagactc ctccttttct tcctctactg
     6841 ctcccactac taccaatacc gctaggagca ttactaaatt ccgttacccg aggtgtgcca
     6901 tcaaatccta tagccactga ataaccatac acaaatggct ctcttacttt aagaccttct
     6961 gatggttcat attcacccat tagttctttc gatgcttttg tttcgatttc catgaatgta
     7021 tcttcaaatt ctctctccgc ttcttcaaaa tctctaaaga actcagcacc aaagcaccta
     7081 ctattaaatc ttctaaacca atcatatgag tctatatcgc agttacacat atcattcaca
     7141 tctccttcaa cttatgtata ttagtatata tcataattaa attgactagg taaggtaatt
     7201 cttggtcaca tcaaaatcag aaaataacat caatgtctcc tatccaatcg ccctaaagcc
     7261 tcgatagaaa tgattatgga ctaattagtt tttttaccat taaacgcccc cagacatcca
     7321 agccaaaatc tatatcttga aactagatac tccatagtcg tttctttgtg gcatctgttc
     7381 aaataacatt ttcacgcttt ctacaaagtc agtgaggatc ctgttatttg gttggtaaaa
     7441 agcatcatat cgccttttgt ttcccagaat gtaagaacaa tcgattcttg cgaatctttt
     7501 agattgtccg ttattagaaa ccccttaatt ccttttactt ttcctgtgct tctctcttac
     7561 caatctttaa tctagctctg ttatgtttga tgtatttaat atcttgtcta tctaacataa
     7621 tacataaaac agacgcaatg attcatactc taaaggtttt tggagttact tatactacat
     7681 gcttttcgtt atcgtgttca ccaagaggtt tttcagaatg gccttgccag attcttaatt
     7741 gtagtattag aaccgtatat atactaggat ttgtagagct cgcacagtga acatctacat
     7801 tgtggtagag acaaaattta agagttaaga agcaaaggac acacccagtg agaaatagca
     7861 aagatactgt aggtggactt aggatccatg aagagatctg aggcatctgc gactacaagc
     7921 aaaattgaga atcaaaaaaa cgttgacgaa agactacctg aagaatgcgg gatgtgtcat
     7981 attggagtta acctaataca aataaagcct gacgaaccgc tgagacacta gaagtagacg
     8041 agaaaacaaa gatacaagcg ttatctttgg ctaaagaatg ttatcaaatc cattggttgc
     8101 aaattacaaa ggttgtatat ccatcacaac gtcaaccgat gtgttcattc tgccatgcca
     8161 atgtgtggac aattctgacc ttggaaaaga cgctttgtcc attttatcaa tttcacctac
     8221 cttcttgcct tggcaaatta tagtatataa ctgaagaata taacaaattg tactttcgtt
     8281 gtgtagtttc tcttgcacca gacttatctt ttctaagaaa caatgtgcat tcatgtctaa
     8341 caacatagac tgcagcaatc ataatcggga
//
LOCUS       DAOR01000295            7840 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_59909, whole genome shotgun sequence.
ACCESSION   DAOR01000295 DAOR01000000
VERSION     DAOR01000295.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_59909"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5775..6187
                     /estimated_length=413
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2316 a         1416 c         1678 g         2017 t
ORIGIN      
        1 gattgtgtga gacagtcatt tcaaatcaac agggaggatt cataaatcag aaacctaatg
       61 ttgctcaggt ttcataaact accatatagc ctacgacatg atagaataat ctcttttatg
      121 ataactacga tctattgact gtctccgaga cctttgtttc tgtctacgtc ctcacaactc
      181 tgtctactat catcgtgagc gccgtgctat aactttttct ttattatatt cgaactgcaa
      241 agcaccacag gtacgatgga accattactt tgatactgga gctgtttgag aacttggcat
      301 tagaagaagc atcataagat ctcgcagctc acggaatggc agggtttgat aatggaaatg
      361 cagggaaaga tcttgaaatt ccggatagct atgaggtact gccaatgatt gcaaagacac
      421 ccttagttgc tctaatagat gatttgtttg tgttcttgta gacttcctta cgacacagtg
      481 gattgatact gatgaggatg aggatgatga ccaatcactt tttctaaaaa taactctccg
      541 gctctatagg agcttctaac gagtgggcca gctacaacat atttgaatcc cgttcgttcg
      601 gtagtttcct taaaccattt gaacgtctct ggagtgacgt attgaactac tggcagatgt
      661 ttgtatgtcg gttgaagata ttgtccaatt gtcaatatat ctacttcagc cgaccgaaga
      721 tcgagagcag tctgcataac ctcctcatcg gtttcaccaa tgcctagcat tatggaagat
      781 ttcgtaaaaa tttccgaatt aaattctttg atttgtttta acactcgtag cgattggctg
      841 tatgatgctc ttccatctct gattttatga ctaagcctca aaaccgtttc aatgttgtgg
      901 cttatgactt ctggcttgga gctaactatt ttttccagtg aggagatgtt tccttgaaaa
      961 tctggaatta atggttctac aatagtcttt gggcatagca tttttattgc ttttacagta
     1021 tttgaaatat gaccagctcc tccatcctcc aaatcatctc tgcaaaccga agtaattact
     1081 acatatttca gtccccattc tttaattgcc ttggctactt tcaatggttc cgttgggtca
     1141 ggcaatgcag gtttatatcc gctttttaca gcgcaaaatc tgcaatgacg cgtgcagagg
     1201 tctcccatta tcatgattgt agctgtacca ctcccccagc attctgctat attcggacat
     1261 cttgcctctt gacaaacagt atgaagattc agtgtagaaa gtgtttgttt gacattcaca
     1321 tagttgttat ccgacggagc ttttatcctc agccattcgg gtttctttat taggatgtct
     1381 cgcacgtcca cacgtaatac agactgagaa ccatatgcac aataataagg ctttactgat
     1441 tggtagcagc agtagtggtg ggagtgtgtc ctgtatacaa tgaagacacc tttttacgaa
     1501 aagcacaagt cgctaaatgc aagaatggta gactttcacg gttggcttat gccattacag
     1561 tactctggga taattagcga gcacatgagt gttagaacca gagcaggttt gttcgatgtc
     1621 tcacatatgg gaaggtttaa agttgaagga ccgaactcgc tggaattgtt gcaaaatttg
     1681 atgacaaacg atatatccac gttgcaacag aaccaggcgt tgtactcgct aatgtgttat
     1741 gatacgggcg ggattgtgga tgatttgatc gtctacagga taaatagaga aacatatctt
     1801 ctggttgtta attcatcaaa tatacataaa gactttgaat ggattcttga gcattgccct
     1861 gaaaaagcag aatcaacagc aataaaaata gaagacatta ccgatagtat ggcactgctt
     1921 gcattgcaag gtcctttggc atataagctc ctgcaatcga ttgtagtgga tattaatttc
     1981 gagcagctaa agccatttaa cctaataagc acaaagatct ttgatgtaga atgcatcgtc
     2041 tcacgaacag gatacacggg agaagacgga tttgaactct tctttaactc ctcagaagta
     2101 caagtttggg ataagctttt gcaggtaaat gcgggacttg acataagacc tgcgggactg
     2161 ggcgctagag actcactccg gctagaggca ggcttattcc tttacggcaa cgatatggat
     2221 gaacacacaa cgcccttaga ggtcccgttg gcatggaccg tcaagtttga caaaaaacaa
     2281 gacttcatcg ggaagaaagc attgcaagta aagagcgtga ccagaaaact cgtgggtttt
     2341 gaaatgctaa gcaagcgaat cgctagaggt acaaatgagg ttttaatcgc tggatcaaag
     2401 attggctggg ttacgagtgg agggttttct cctacactta ataaatcgat aggcttttgc
     2461 tctgttcctt cagatgttcc tttggaccag atagttgaca tcagtattac tagaggagcg
     2521 ggaataaaaa aagaaggaga ggaaagacta gaagatatta cagcaacgcc ggctgcagct
     2581 gacaaagaaa acctataccc ggcaagaatt acaagtaccc gtttttacaa acgaaataaa
     2641 aaaggttgaa tgagaatgag aatgagaatg aataagtaag tgagtgagtg agtaaggatt
     2701 tatactttat actactatac tcgtagcgct ggatgagcat gcctgaacct actattagat
     2761 ttacaagaga gcatgaatgg gtagtaatga gaaatgaaat tgcgacagtt gggatttcgg
     2821 attttgccca agatcaactg ggcgaaatag tttctataga acttcctaaa gtcggtagca
     2881 catttaaggt gatgcaggca atggcaattg ttgattctgt aaaagcgtca tccgatattt
     2941 tttctcctat gagtggagag gtatctgaga taaatgagaa tctccttgaa cacccggagc
     3001 taatcaacca atcgccatat gaatcaggat ggatgctaaa gataaaaccc tcgcagcaac
     3061 gggaatttga tagtttgatg acaaaggaag actacgacag gttcgttggc gaagaaagag
     3121 acaaagaaga caaagagagg agagtgtcaa ataagccaag ttaaggctaa ggctaaggct
     3181 aaggaaagga gaagatgata acaatatgaa ttatgtaccc accacaaaaa aagacgaaaa
     3241 aataatgcta gaaagggttg gagtaaagtc agtagaagag ttagttgatt cattccgtcc
     3301 catgatttca aatgagagat tggatctgcc tcccgcattg actgagatgc agttaatgca
     3361 acacatgagg aacatatcca agggaaataa gattatgagg tatttcgttg gagccggctc
     3421 gtataatcac tacatccctt cggctttaaa ccatctagtg atgagaggag agtttttgac
     3481 tgggtatacg ccatatcaag cagaaattaa tcaaggaaca ttgcaggcaa tgtacgaatt
     3541 tcagtctttc atatgccttc taactggaat ggatgtgtca aacgcttctc tgtacgacgg
     3601 agcttcagcg ttggccgaag cagcccttat gtcagcatcc tacactggga gaaaacaaat
     3661 ttcagttgga aacaatattc atccacaata ttttgaagtt ctaaagacct attgtgaagc
     3721 tgcagacctt gagatcacta tgaatcaagt tacccaggag actgcatgta ttctgacaca
     3781 aaaccctgat ttctacggaa atgtcgagaa tctatcctac atatcagaaa gagcacataa
     3841 agtgggtgcg ctatccgtta cgtgcgtagt tgagcctact tctcttgcaa taatagaccc
     3901 tcctggcaat tacggggctg atattgttgt aggcgaaggg cagtcctttg gtataccgct
     3961 aaacttcggc ggaccgtacc ttggattttt ggcagttagg agttatttgt taaaaaagat
     4021 tccgggcaga atatgcggta tgacgaatga ctctgtagga aatagaggat ttgttcttac
     4081 acttcaggca cgcgaacaac acataagaag agagagagcg actagcaata tcacgacaaa
     4141 tgtggcctta aatgccttgg ccgcaacagt atacctggca ctcatgggta ggagcggctt
     4201 gattagagta gcagaagctt catacaggaa cgctcatttg ctccaaagta agcttagcgc
     4261 actaggtttt caaacactaa acaacttacc attctataat gaattcctag tgaaaacacc
     4321 aaagtcctcc aaaaaaatcc ttgctgattt agcaagacaa gggatctgcg gagggttaga
     4381 ggtaggagac gacaggcttt taatttgttg tacggaaatg aacacttctc aagatataga
     4441 cgattttgtg tctgcagtag cagaaaaaga acaaacggca tgagtaacgc cgatgaccgg
     4501 attggagtaa gagccgaggg ggaaatggag aagctgctat ttgactatcc tgagggatgt
     4561 attacagagc acgttccatc ttcgtcattg gatcgttcta tcactggttc taattatgca
     4621 gacgtccaaa tcgaaattcc agacaacatc aaaagaaaaa ttcttcccat tccaaatctg
     4681 agcgagattg acgtcgttag acatttcacc aagctgtcca gaatgaattt cggtatcgat
     4741 gtgggtccat atccacttgg ttcttgcacc atgaaataca atccaaagat taacgaggat
     4801 atcgctagac tggaggcata tgtgaatctt catccctatt gtcctgaaga ttgtcaacaa
     4861 ggctcgctgc gactaatgta tgagctcgaa caatatctca aggttcttac tggcatggat
     4921 gatttttctt tgcaggcggc agctggttcg caaagtgagt tgatgggtgc catgatggcc
     4981 aaggcgttct ataaggaaaa ggaaaaagaa aaacaaaaga atgaaggcaa cgggaagaag
     5041 aggacaaaga taataatccc agattctgca catggtacca acccagcaac tgctgccatg
     5101 tgtaagttcg aaatcgtatc cattcctact gattcgcatg gcaatattga tctcgataaa
     5161 ttcaaagact cactaaattc cgacgtagct cttgttatgc tgaccaaccc taacactctt
     5221 ggtctttttg aacaagatat actccagatt agcgaactag ctcactctaa tggcacgttg
     5281 atgtactgtg atggggcgaa tatgaacgct tttttgggca tcaccagacc aagagaccaa
     5341 ggctttgata tgattcacct taatttacac aagaccttct cgactccaca cggtggcggt
     5401 ggaccgggtt caggagtact aggagtaaag tcttttctag ccgactatct tccagttccc
     5461 cgtgttcgag taaggaaaag tccttcgaag caatcccgga acgaaacgga atctgatcga
     5521 aaacaaaatt ctgggacgaa taacaactct gatgattatg attatgatta tgataataat
     5581 tattattttc ttgatttcac aaaaagaaaa tctattggta aggtcaaatg cttttacggt
     5641 aactttggga tgcttctaag agcatatagc tatatccggg cttatggctc caatataaga
     5701 agggtttccg aaaatgcagt actaaatgcg aattatcttc taagcttact gaaggatgtc
     5761 tacgagcttc cttannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnncta atcgttcctg aagcgctaat gatagagccg actgaaactg agagcaaaga
     6241 gtctctagat tcatatgcat ccgctttacg aaacatcgct aaagagttgc gcgagaagcc
     6301 agatcttgtc agctcagctc ctcacaatac ccctgttaga aggcttgatg aggtactggc
     6361 agcaagaaaa ccgaatctgc gatggagtcc aacatcagcg ttatcgacct aaagcttcgg
     6421 gatatggtga cagtactagt aggcaatcag atggtagtaa tagtaacagt aatagtaaca
     6481 gtaatagtaa cagtaatagt agtaacggta ggcagcgtaa tagtagtaat aatagtaata
     6541 atagtaatac cacttcacga tgcgacggca catggtaatc acacttgtta aagaagctgc
     6601 attacgttta caaatcaaat aaattaatcc atatcttgct agagcatacg acgaagactc
     6661 caacgcagaa ctgcaccatc tctacaataa ggataacagg agacttcttc gtctatcctg
     6721 aagaagccct ggataaactc gaagccaatc tagttggtac caaattagag agatatgctg
     6781 tcaggcaaaa ggtatggcaa tggctacagg gatctcaggt cttcggtttc gattcagact
     6841 ctctgacaga ggcaattata ggatgtttcc aaagtggtga ggataatcat aaaatttagt
     6901 gaaatcaggc ttctgagaac tggccataac aatgcgtact ggaatatggc tgtggatgag
     6961 gtgcttatgc agagcgtcag gaggagtact gccgagactc ctaccctcag actatatgga
     7021 tggagccctt ctgcagtatc gattggatac tttcagagtg tggaacagga agtaaataca
     7081 gaaatttgca agaaaactgg tgttgacctt gtcagacgag ttactggcgg cggtgcggta
     7141 ttacatgatt ccgaactaac atattctttt ataacaagaa aatatccgca aaatatactt
     7201 gaatcttaca ggttgatatg tgatcctatc gtcagttgct tagtaagtct tggacttaat
     7261 gaaccgaggt ttgtaccgct aaacgatgtg cttgtcgagg gcaaaaaagt atcagggaac
     7321 gctcaaacca gaaaagaagg tattctgcta caacatggaa caattttatt ggatgttgac
     7381 tttgagaaga tgtttagcgt gttgaagata acccagcaga agattcaaga caagctgatt
     7441 aacaacgtaa agcaacgggt gaagggtttg aatagaacat ttgaggaggt ctcggctgcc
     7501 cttgagcaag cattcagtac gaaatttgct gccaagcttg tagttgacaa aattacatca
     7561 caagaatgta aggacacccg gatgctgatt gaaacaaaat atggaactca ggcttggaac
     7621 tggagaagat aaaagtgata agcagtatgt cgggccattc cacaaacatt tgttcgtgtt
     7681 aaggcttatt gccttggtat ttggtttatg atcatataac atgtacgacg tggtagtaat
     7741 tggagcaggt ccaggcggct acgcatgtgc tattcgggca gcccagcttg gtgcaaagac
     7801 atgcataatt gaaaaaaatg gaatgggtgg aacatgtacg
//
LOCUS       DAOR01000296            4400 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_81646, whole genome shotgun sequence.
ACCESSION   DAOR01000296 DAOR01000000
VERSION     DAOR01000296.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_81646"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3904..4093
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1171 a          779 c          818 g         1442 t
ORIGIN      
        1 gtctatatct tgatgtagta gtgtaatcaa atatgttatg aaagtcctac tacctacata
       61 aattagcatt ctgaagttcg actgaatgct aaggacgata tggaatcata tgtgcagtac
      121 tgctgctact ttttatttag gaggatattc ttccagtgga aaaatgcccc acgaaaaaat
      181 tgaggtgcat gcatgtctgg ttcataatgc ggcttggcgt ttcacgataa ataaagttag
      241 ttcattgcac cagaagatta tacgtcacat ctactgaata gttacttgag ccggtagtag
      301 gattggcggg tgttaatccg ctaaagtgga ctacatgctt accaggagcg agaggctgca
      361 gaaatccata ccatccatct actaccgcct gtgtggtccc tacaggcgag ccgaaaatat
      421 tattcactgg aaatgtcaga ttgaaaagtg gagaggttac tctatatttg tcaggttgtt
      481 gtagattcac tccatccacg cttgcttgca agttggtagt tggattattc tggactttag
      541 cacactcggc taaatcggat tccgttttaa gcgtagggcg ttctgagtaa gaacattcag
      601 catttattat tccaaataat atagccttcc ctgcagggat agtacaagtc cgagtggcag
      661 cgcctcccgg tgtgcctgcc aagaaccaaa cagggccctt ctggttctgg ccacagttct
      721 tgccggtctg atctgttgcc ggattattta cttgcggttc ggaaagtaac cattgctccc
      781 acttagcagt ccagtttcca taactgacgc catatggctt agagttaatc gagtaaacac
      841 ctgggttgat actgtcacct cgagcgaaaa gaatcataga agacgtcgca gaagtaagtg
      901 ctacaactgc taaacccaca acaataagcg tttgtagatg atttactgat ctcttatcaa
      961 taccgttgaa ttttgtgctc atacccgtag tgagcaatct atggattata agaattaatg
     1021 gttcggtttg tcagtttacc tttaattaat aattaaagat tcgtccggaa taaacattcg
     1081 taaactcaag taacaatttt tgaaaatcaa aaagtgttgt taggctatag agtagacttt
     1141 cagcgaaacc agtcggctct gatactgatt gcagcaaaaa tagaaacact aatgataggt
     1201 tatcttctgt gatctaattg gtaccataag gtggtcaggt ttgattttct tggatccttt
     1261 tcaaattctc cacatagaaa caagatcgtt ttccttctag tttctatcat atttgcatcg
     1321 ctaatgctag agttgtctct aatcaaaatt tattccatat ctgtcaatga gttgatttcg
     1381 aatatgggta tcacattctt tgtaggaatt ggaatcgttt atgtgattgg acagtacctt
     1441 cttttgaaat tcgttaaggg atttacgaat aagagaagga tgggtaagga gttgcatcta
     1501 aatctggccc gtagatctgt tactgtagga cagtacatat taactgcaat tcttgtgttc
     1561 gttattttgc aaatggtctt gacatcccgt tacagtgtta ttacattagt tgcagttact
     1621 agcatcagtt gtgtgctgtc gatagttatg ttaggcttgc ttgcgcagaa attcttttca
     1681 tggttcaaga caagaaagga gaaggtaata ttgttatatg gtctgtcttc gtcgatgttg
     1741 gcactaaacg ctattctcac gattgcttac gtggatcttg tactgacgga attacctcct
     1801 tacataagtc cacatccggc gcttcaattc tttcctttta ttgcaccggg ttctgtgaca
     1861 gatatcctgt actctgggtt tgttgtctcc tcgattctgt cattcatgtt gacttggagt
     1921 gccactgttt tacttttacg ctacctttcg caaagattgg gaagagccag atattggata
     1981 attctcagcc ttccctcggc ttattttttg atccaatttc tgcctttgtt ttatgatgta
     2041 ttttccttat tctcacaatc tgtatctgtt ttttacttgt atacactgtt tttcacgttt
     2101 agtagacctg ttggtgctat cttatttgga cttgcttttt ggatgatagc aagaagactc
     2161 aggcagagtg gtactgatat ccaaaattac atgatcatct caggaattgg tcttgttcta
     2221 ttgtttgtat ctaatcaggc gattgttttt gttgatgagc cgtaccctcc atttggattt
     2281 gcgagcgttt cattaatggg attatcatct tatttggttt taattggtat ctattcttct
     2341 gcgatttcta tatctgaaga ctcaaggttg cgtaattcaa ttcgaaattt cgctctgaag
     2401 gaagcaaatt tgttagatag tatcggctcg gcccatatgg agcaagagat acaaaaaaag
     2461 gtaatagaat tcacaaaacg taatcaagat agaatagcag aagaggccgg ggttcaatca
     2521 tctcttacag aagatgatat gaaatcatat ttacaacaag taattagaga aattcagaat
     2581 gcgaggaaag gtaataataa tcatcacaaa taatgatggc aaagaaaaga tcagaaatta
     2641 cttgtgcctt gtgcggaaag ataataattg gcaaagatag acagaaaatc aaggaaggat
     2701 aattgttgag attattgacg atacacgtta tactttcgac agcaatgatt gcgtgcaaat
     2761 gttcaagaag ttcaggaatg tgtatgggac ttcatttttt tagttttagt aaaaattgcg
     2821 ttattatata gaatttaacg atgctaagtc catagggtac gattaatttt tgcattcttg
     2881 tttttccacc tacgtttact gtcgccttta tttttaacct tgtattgact tcctgatatc
     2941 ttatcatgaa tatctcaaat catcgcgcat caagaacatg atatgtttta cattttctca
     3001 ttctggatgt ctcgtataac aactgcttta agagttgttt ttcctagaat caattatttg
     3061 cttcatgagt tcttgttgct tatgggcgtc ttgttttaat aatatcttat acgcttctgg
     3121 gtcatatttc agccatctac catcttggtc agggacaaac ccacgacctc taaattgatt
     3181 ttgagaatta agtcctagtt cacccgcatg cagcacttgt tttaattcta ctatagttgg
     3241 atctatgcta aggaattttg caataagtcc cttaggccat tgaaaggttt tatcacccca
     3301 ttggaaatgt aattgtatgg tatagagtcg ttctagatat ctcactcgcc ttcgtagtcc
     3361 tacctcttca ggcgtaggac ttcctttatt ccaatttgta cgcttacatt tagcacatct
     3421 tgtaggttca ttaccgtctc tcccgttaag tcgatttatc catttataac cacatttgca
     3481 gcattggtat tctgtgactt gtattctgtg agttggcata atatatctca ctaacggtta
     3541 tacattttat atgtctaact attggtgagg agtttaaatc gaggcctttt tcggtgtaga
     3601 tgtagaaagc cctgtaaaat cctgttgatt ggatgtctat gagtgctttt tatcgcccta
     3661 atttggctaa acggcattag atgtaactgt attagaacca gaaaataata tttattatcc
     3721 gttcatatgc gttacacgca tacttcaata atagaatact cagtttagtc aggctatagt
     3781 aacaatcctc taactgttat ctgttccagt atgacaacca gttagtgaag aggaaaacaa
     3841 tacatcttag atcggattgc tatatttcgg aagttctcat tcttactcat atctcaaata
     3901 tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnctcaaat atatcaggac cgcctttccc tgtagtagcc tctcttttag
     4141 gatttacttc atctatcttt tttgatagtt cacctacttt cggatgactc aaaagacggc
     4201 taatttcatc caattctgaa gcagacctga taacaactcc tcttttcttt gtgatagaac
     4261 cgtacgaatc aattggatta atttcgattg ctagagatgc agaccttcct ttggaagcag
     4321 gtagtctgaa taagaataca ccaggtatat tagtagcctt catttcccaa tttcttccat
     4381 cattgagaaa ttgtctcagc
//
LOCUS       DAOR01000297           21626 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_3254, whole genome shotgun sequence.
ACCESSION   DAOR01000297 DAOR01000000
VERSION     DAOR01000297.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 21626)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21626)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21626
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_3254"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    18880..19143
                     /estimated_length=264
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5999 a         4563 c         4248 g         6552 t
ORIGIN      
        1 agtccttaat aatgtgataa gctccttatt gtctctcctg catacaatcg caattgcacc
       61 ttggcctgga gctgggacaa agtccttgat gtcgaacctt tcagcgataa catctttcat
      121 ccctagtctc gtcagaccag cctctgcgag aattacggca tcatattctc cagtgattga
      181 tctgctgact ctggtctcta catttcctcg aataggttta acatgcaaat ccggcctgac
      241 cctgaatagt tggatggccc gtcttaggct gcttgttccg actattgcgc cggtaggcaa
      301 gtctgccagc ttcaaatctc ttttactaac aagaacatca ttgggactag cacgctttgg
      361 gatacttgct acaatcaatt tctccgatag atcagaagga atatctttca aactgtgaac
      421 tgcgaaatca acttgtcctg agataactgc ttggtttatc tctttttcaa aaattccttt
      481 ttcgtcaatt gtaaatagtg gtcttctgtc gacatcaccc cttgtactaa tggtaatgac
      541 ttcaaattca agtgaagttc tcttcctcaa tgtacaaagc gtgatctccg tctgagcgat
      601 cgctagtcga ctacctcttg ttcccactct aaagttgttc attatgactg tttcacctcc
      661 cgcagcgact catagtatga ttctacagtt ttatcagcat cgtcttcgct gtgggaataa
      721 gatataaagc aagtttctaa ccgtgatggt ggtataaaca ctcctctctt taagagctca
      781 tcaaaaagtt tcctaaacat gattggatcg gacttgttcg cactaactgc attcttcact
      841 ttctcgtctg taaagaagag ttggaacatc gaaccgatgt tatttatcgt acaatcaagt
      901 ttgaaatcct ttaacgcatc tttgatccca gatacaatgt tgtcgcaggt tctcgcaact
      961 tttggatata tctcgttctt tgccttacct aatactttga tcgtagcgat actggctgag
     1021 acacaggctg gatttccagc ataggtactg gcttgataca ccttaccgct cggagaaagc
     1081 tgttgcatta tttctctctt gccggcgatc gcggcaatag gaaacccgtt gcccatagct
     1141 ttcgcaaaag tcgccaaatc cggtttgatt ccaaagaatt cgcctgctcc cccaagagca
     1201 agtcggaaac cggtaacaac ttcatcaaaa attagtacaa tatcgttttg tcgtgtaatc
     1261 cttctaatct cgtttagata ttgcttttct ggcaatacta gtcccatatt tgcagcgacc
     1321 ggctcaacta taacgcaacc aacatcgtca tcttccttaa ctacctgttc tagtgtttcg
     1381 gaatcattgt atgggagcac cagggtctgt gaggatgctt cattaattat gccatcagat
     1441 atacttgcgt cgacgcctgg cttcgggagc acataatcgt acgctccatg ataacatccg
     1501 tcaaatttca caaccttttt tttttttgta aatgcgcgag ctagtttgat cgcgttcata
     1561 gtggcctcca tgccagtatt cactagtctg atcatttcgg cgcactgcac ggccttcgaa
     1621 attaactctg ccaattggat ttcctcttca gtcggaacac agaacaagct tccatcatca
     1681 atccgggact ttactgattc ggttactgtt gaataactat ggccaagcaa tagcgctccg
     1741 tatcccatac aataatctat ataagtctcg ttatcagccg tgcgcatcgt acttcctttc
     1801 cctgacacgg caaagaatgg gaatggatca tagtaacgta caggactgtt gacacctgag
     1861 ggaatgaccc tctgggctct atcgaataat gattttgagt tgctaagcat aatacactgg
     1921 gtgttgcctt tctaaaatat tataattgta tcgcaatatg cgctcaattt tttttattcg
     1981 ttcagcagtg ctgccatttt ctttgcgtaa tacgtaataa tcagatcagc gcctgccctc
     2041 ttgatagaag ttagagattc ggtcataacc gagtcctcgt ctatcagccc cttgccagcc
     2101 gctgctttta tcagagcata ttctccggaa acactgtacg cacaaattgg taaattggta
     2161 ttcttttttg cctgataaat caggtccaaa taggggatgg ctggcttaat cataataata
     2221 tcagcgcctt cccgaatgtc ctgttcgatt tcatgcatcg cttctcttgg attagtaaat
     2281 gacatttgat atgtcctcct gtcgccgaat tgaggccgag aacaagctat atccctaaaa
     2341 gggccatata ggggagaagc gtgttttgca gaataggaaa tgatagcgac gtcttcaaat
     2401 ccattttcat ctaagccttc acgaatagct tttacctgtc cgtccatcat ggctgacggt
     2461 gcaaccatat ctgttccagc ctctgcgtga ctcgttgcta ctttggctaa cgtgcgtacg
     2521 ctcttgtcgt tatctacctt tcctttgact acgatgccgc agtggccgtg cgtggtatat
     2581 tgacaaagac aaacgtcagt tatgatagca actctacttc caaagtgttt gcgcacaact
     2641 ctaaccgagt cctggacaat cccgtgctcc aagtacgcag acccatctct tttattctta
     2701 tagaacggaa tcccaaacag cataatcgct cgaataccaa ggtctaagac agtctgtacc
     2761 tcgttgatga tttcggaagg aggtatcctt tctatcccgg gcatagattg aatggtttca
     2821 cgcttggtta tccgttcttg aacgaaaatt ggggatatga gatccttgac tgaaagatgg
     2881 gtttcttgta ggaggtctct gacagaaggt gttgttctaa gccgccttaa cctaacaacc
     2941 ggatagccag caccgcaatc atacctaccg atcttcatga tagttgaaca acctggctgc
     3001 aattctcatt acatcttcat ttttactagc tgtacctaat tcttttctga gactattcat
     3061 tggtgtggac ataatagcct ctaccatcgc gtatgagaat tgttccatta ttctacgttc
     3121 ctcgggccct atcttagctc cgaggataga aatagccttc ttgagttcgc gttctctgat
     3181 cctatctaca tccttaaata tagaaaccac cataggctca acctcctttc tcttcaggac
     3241 catatcaact agtttcatct cttcatctat tattctctgg gccgacgcaa tctcattttt
     3301 gcgggttcga atattccttt caactatctc cgtaatctta tcaatgttaa ataatttgac
     3361 cttctttatg gttgcgactc tctcgtcaac ggttcgagga tttgaaaggt caaaaaccac
     3421 tagccattta tctctcttgg ccatggcctt ctcaatccta tcataggtga ttaagtaata
     3481 aggtgcggta gtagaaagaa aaattagatc gacctcctgg aacatttgca cggctgactc
     3541 aaatggaacg ggattgcctc ctactgtatc tgcaaatgat ttggctctct caaatgtcct
     3601 actagtaatc ataaaatgag cctctctttg tttcaaagat tttgcgacga ggcttgctcc
     3661 ttcccctgaa ccaatcaaca atacgcttct attgttcaat ttgtcaaagt attcctctgc
     3721 caactttact gccatcgagc ctatagaaac gctaccctta ttcactccag tagtggttct
     3781 gactttacca ccaaccttga cagcccgatc aaagataact gagagatgag gacccgcata
     3841 cctgtttttg cgagaaaact cgaatgcacg ttttacttgg cctaatacct gatcttcacc
     3901 gatcaccaat gaatccaagc ctgatgacaa ttttaagagg tgcaatatca cgtcattacc
     3961 attgcaaatt tcaatattct cagccacttc gttataagat aaccctgcag cacttgccca
     4021 tccttccaac aatctttgct ggtcgggatt tctgctcgca gcaaaaagct caacccgatt
     4081 gcaagtctgt atgataatgc actcttctaa ctcggctatt tcaatcactt tttggtgtgc
     4141 ggcatttaaa tcagcgaatg caaatcgttc gagaagatgg atcggagcat tgcgataagt
     4201 aattcttgca ttaattacct gtagtgactg tgagagtgtc gatgatgttt tcatctgtta
     4261 ttctttctct cccattcacg caatagctct aaggtcgtac ttttagcatc ttctgtctta
     4321 tgctccttaa taaggttctg gatacgactg ttttcgatca aagaatagag aaactttttc
     4381 ctttgttcaa ctgtcttgaa tgtcgatttt gcagcagcac gggcgaattc ctgtaattta
     4441 gtattttcga tatcggtttt ttttattacc tttcgaagca ttctctctgc tttaattctc
     4501 acccttcgcg acattatagg actcttgccg gatgtggata ttgcgacttg cattacaccc
     4561 tcgatattaa taattgaggc atatgaaaag tcactaatgg cggggtcatc tacagcatat
     4621 acgaataagc gcatggaacg tgccttctcg acaagcatcc tgtttaacac tttatcatta
     4681 gtggcagcaa gcaccagaaa tagattatta taattatcaa gtacacttgc gtcttttatc
     4741 tttgttttta caagttcgat tcgtccttgt tctctcagtt gagacaaata acgatttaaa
     4801 cgactacaca taacggtgat tttacaattt tgatccaaga ggccacgaat ttttctagca
     4861 ccctcctttc ccccgccaag cactagtaca tgtttgcggt caagatttaa atcaactatc
     4921 aatcacacgc acacaccaga cagtggtgat aagaaatctc taattcttaa tttaattgtt
     4981 acatccccac gcgcttttca gattaataag ggacaaattc tgcaggtctt tttcagctaa
     5041 agaaggcgct cattctctaa ctcaaaacta attagtcgcg ctatcggcta ttgggctatt
     5101 agtcttggaa aacgacaacc ctcttaaagt gcagttgagt ctcgatgcta tagaacaaat
     5161 cggtccggct actaagactc gtttagagca agcgggtttt tcctcaatta aagatctagt
     5221 tgttagggga cctgtggatg tttcagaggc cactggcatg gaaattgaaa aatccatata
     5281 tctctgcaat caggcgagaa ttaggctaga aaatctcggt atcattgaca agagttttgt
     5341 taccgcaact caactctata acaaaagaaa aagagaagat cgtctttcaa ctggatcaaa
     5401 gaacctagat gatctctttg gaggcggtat agaaacaaga gcagtcacag aagtctatgg
     5461 cgagtatggt acaggaaaga cccagatctg tcatacgtta tgtgtgatgg ttaaacagac
     5521 caagtctgag ggaggactaa ctggtaaggc actctatatt gataccgaaa atacattcag
     5581 acctgaacga atagtgtcga ttgccgtagc aagaaaaata gatccgcaga ttgcccttga
     5641 aaacgtaata gtcgcaaagg cgtataacag tgctcatcag gaattgatta tccaagaaag
     5701 cggccccatt atagatgaga agaacgtaaa attacttata gtagattctg ccgtggcaca
     5761 ctacagagct gaatacatcg gacgcggtgc gctctcggag aggcagcaga agcttaataa
     5821 atttatgcac attttagtgc ggatcgccga aacctacgaa gtcgcagtgc tagccactaa
     5881 tcagatccaa tcctctccgg acgcaatatt tggtgatgca ttccgcccta caggtggcca
     5941 cgttgtggca catactagca catatagagt gtacctaaag cgctctggga aaaaccggat
     6001 cgcgagaatg gtcgatagcc cgtatcatcc agagagtgaa gtattattta ctttgtccga
     6061 acatggggtc tctgacccct cagtgtcaat cagatgatta aataacataa cataaattaa
     6121 ggccttaaag catcaattta cgatgccaag ttcacacggt acgaggagaa aatctcgttc
     6181 tgtcctgact aaacgtaatg tctctagagg tatatcctat ttactaacag aatataacgt
     6241 tggtgacaaa gttatcattg atatagatcc ctctgaacat tctaccactc cgcacagaag
     6301 gtttcaagga agggtaggag atgttatgga ggtaggcagg agaattttga aagttgcagt
     6361 attgtttgga agtaagcaga aaattttgca aactagactt aaccatataa agccgtttaa
     6421 cggagggaag tcggtaaaga gtgactgaca ttcttaaaaa agaaataata acattgtctg
     6481 aggctaaaga agttttagaa caggttaagt cagatgatat ggaccaaata cagagatgga
     6541 cgcatgacta tgttgtaaaa ttcgcgaaaa ccggctcaga ggttgcacgt gaaatggtaa
     6601 agcgactaat tgaagagtgt gaattaaaag aagaagaagc aatagaagtt gtaaatgtat
     6661 caccttcctc tctggaagaa ttaagggctt ttactttcgg gtggaaaaaa cttattctta
     6721 ccgaaacctt gcaaaagatg ctgaacatac tccaaacgcg gaatgaaagt taaataaaaa
     6781 ggtaatcaat agttatacag gagaaaagga aggaaggatc gacattgtca gcgtggctgc
     6841 ggggatatga tagtagcatc gggactacta gcgagcagag ttctagaaca gcaccgcagg
     6901 aaggccaacg caggtacgag gagtatgcgt acgttttgga cttcacccaa cgagccaagg
     6961 cgttgacagt gagaggaagg gagggtatga ttattcaggc gataggagag gagcgtctca
     7021 ctttactgga acttctcgga actcaaaata tgagtatcga ggtcgccgag cgagtatata
     7081 ttggtaggga gggccgcgac aaagtgatga gtgtgcttgg caggcttgaa tatgattcta
     7141 tatcccaatc tgcgaaaaac gaacttccta gcattattga aaaaatagtg atagcaaacc
     7201 aaaagcgttt cattgactat ataaacacgg ctcaaccgat tacacccagg atccacgctt
     7261 tagaactgat tccaggcata ggaaagactt acatgatgac cataattaga gagcgagata
     7321 ggaaaaaatt tgaaagcttc gtagaaattc aacagagagt gggattacgc gatccggcta
     7381 agctgatagc aaaaagaata atcgatgaaa ttaccggtga agcgagaatg aatttgtttg
     7441 tcaagaaatg aaactctcaa aacgaaggct tctcggccag catatgctta aagacggaag
     7501 agttcttgaa aaactaatcg aagtagccaa gattagtcag aacgagaccg tgtatgaagc
     7561 cggaactgga gagggtattt taacaactga actgtgtaaa agagctaaat cggtggtttc
     7621 gttcgaggtc gaccgcgacc tgttcaagaa atcaagcaag ctcatatctg cttttccaaa
     7681 cctgaaatta atcaatgcag atatttttaa attccccgtt tgcgtgtttg atgttttcat
     7741 ttcgaatctt ccttattcta gaagtaggga tgcacttcaa tggttagcgt tgcagaaatt
     7801 tacccgggca atactgactg tgcagaagga atttgcagat aaattgcaag ccagacctgg
     7861 cgatgaaaac taccgatcca ttactgtcat aacacagcac tgcttcatta tagagcagtt
     7921 gtttcatgtg tcaaagggat catttgatcc tcatcctagc gttgagtcaa cggttataag
     7981 acttgctcct aaagaaccag ggatcccaat aacaagggcg acaattaaga atttgaatct
     8041 cctattttca agtagaaata gaaaattatt ttcagtatta aaaaaacaca attgtaaaat
     8101 tgattttgcc ggtacgaaga ggatagacga attacaaccg actcagttat tgagtctagc
     8161 tgaatctata tgacgtttat tctctattgc tgatagcttt cattaacagc caacgacgtt
     8221 aactgtctaa attggatgaa ataatctcag aatgaagata aaaaggagct tttttttata
     8281 tctcttccta aattagttag gactgcctct tcaatagctt accaacccga acagatattg
     8341 gtctgccatc tgagacacag tgggtctatc gttaagatcg taagtgacgt atttaatttc
     8401 gcgataaaat tcgtggtagc gctaaatatt gaattcgaaa tgtgagtgtc tccaagatca
     8461 tctcaaaata tcatccacct ggtgtacgag tcgcttttac caccagcttt ctgtcaccgc
     8521 ccagagccgg cattacatac gatcaactct gcgacggtgt tacgtgttct aaggactttc
     8581 atcgtggatt ctacaagctc gggagcggca ccatctcctc gaatgttgga ggcagttttg
     8641 ttgtggaccg tggaaagtgg tcattaacga agtaatctcg attatttcta ttttgaagac
     8701 tgggaagaag aagttagcgc taatcttgcg accatgttgt tgacaaaggg aggaaaatca
     8761 gatggcttat ttacttttgt aaattcacct ttttctgaat agtaagaata atgaatatta
     8821 tagttttcaa ggcttagagt ttttatatgt ggaattccag atgaattttc tactacagat
     8881 attgtcttat tatcgggaga atagatcttc ataaagtcag ccatcgtcaa cctaatgggc
     8941 cgtatatatt caatgtggat agtggaacct gctgcatcag tatgaatcac atgtaagcat
     9001 tcttttccat taaccgtgct atttccgact ccggcgggta gcgaattctg tttgccgtca
     9061 acgataactt gtactcgtgg atacagatta aactgatctg cattctgtga cctaagacat
     9121 attttcgtcg gtatgggagg cggggcaaaa tgtggaagta taactaatgc tacgagcagt
     9181 gaagcgccca tagcaatagg cggtatcagg acttcttttt ttcctagtct tttcggtgcc
     9241 ttttcttctt ttactttacg tttgggcatt acaaatttac cgcaccctaa aagaatcgcg
     9301 tgcggtatta ttaatattct ctaaatgtga aattccaggt tcatatagaa caacttttta
     9361 ttgttgacag ccaagggatg gatgagaaat atgcagatag ttcagctttc agatatccac
     9421 attgggagcc tgttcaatca aaatgttttt gacacgctag tggaggagat cgagaaccta
     9481 aaacctgatg ctattattat tactggcgat cttactgacg atggattact cttccagttc
     9541 gaacgtgctc aatttgaaat taataagctc gcatgtccta acaagatcgt tttggcaggt
     9601 aatcatgact accggcatac aggatatctt gttttcaaga aattcttccc gtcaaagcaa
     9661 atctatgaac tagaagacga ggtagtaatt atgactcttg gcactgctag gccggacaga
     9721 gacgagggag aggtagggta taggcaaaat aagtggatga ttgaaacctt ggctaaatgt
     9781 gagaacaaaa cgaagattat tgcaatgcac caccatctca taggtattcc agacacgggg
     9841 acagataaaa ttatcatttt ggatgcaggt gacaccttaa tggcctgtct tcaatccaaa
     9901 gtcgatttgg tgctttgcgg tcataaacat agaccttggg tgtggaactt gggatcgttg
     9961 gagattgctt atgcgggaac agccagttca acacggttca gaggattttt cgagaatgct
    10021 tataatattt tagatgtcag gaacggaaaa tcacaaatcg acataaagat agttggcggt
    10081 aaacggatgc ctctcttaaa cctagtaaat aaatataagc catttttgga aactgaaagc
    10141 tagcacaagt atgcggtaaa ataaatccta ctgactcgga gagacgagcg gcagtagaaa
    10201 tctcgacctc tcgagtcaga gactatttat gtctcgctat ctcgggaaaa taatcagcaa
    10261 gcgagaccgt tgttgtctat tcatattgat ggtgcaagtc gaggtaaccc aggttattca
    10321 gggatcggca ttgtaatcac taaagataac gacaaaatcg cggagtataa agaattcata
    10381 gggattaaga ctaacaatca ggcagaatac attgcattga aaaaagcgct ggaaattgcc
    10441 tcctccattg acgatgaaat cacagttctg tcggacagtg aattggtcat aaatcagaga
    10501 acaaacaaat acaaagtaag aaataagcaa ctaaagctgc tattcaaaga aatctcaaat
    10561 ttggaggagt atttcagaat agtgatttac agacatattc cccgggaaat taatagatat
    10621 gcagactcat tggctaatca agcgatagac gaacgaatta aatataaaaa tgccggtacc
    10681 taaattgacg gcagccgtga agactcagag ttaatccgat tttgtttcac agaatatgag
    10741 gagaaggcat ttgactgagc tgccattatt tcatcgtaga agtgctcatc atgtaatatg
    10801 acagcacgcg ctgtctagtc aactctccat gattatttta tcttgttgcg cattatagtc
    10861 cataagatgt attatcaata tgttgcactt tgccagacag ctaaggtaag aaatggatgt
    10921 gtaacctaat aaccaaatgg aaatatctgc gtggcgagtt gtggattgat ctttgatgct
    10981 atgtcgcatt cgatataggc aattatgttg ttgcaagcgg tatcccattt aagaactcga
    11041 aaactgtgtt tattacattc aatacttctt accgagtcaa agtcgattaa attttttttg
    11101 tgatggttat aacgtcctcg tcgatgagca gatgatccgt tcctactttc tgcccaccaa
    11161 atttagcgct cttaccccat atctgggcga atttaaagtc cctgatcata ctacgatgta
    11221 ccttgttgca tacatcgcca acattagatc cgttttgcat aatcataggc tccttaaagt
    11281 cagtttcttt ccccttttgg tgcatgtaaa ttctaatgaa atctaatttt ttgtatattt
    11341 cttctttgag agcatcgata ttcacgtctg catctgctga aatcggaata aaattacttc
    11401 caatttttga ttgcacttcg ctaacaaaac cctggtttac gaggtctatt ttgttcaata
    11461 ccgcaagcga cggaatgtag gatcggttgc cgctcaagac atcaattaat tgatcgtctg
    11521 atagaccttg ttctcttaac accactcggc aattccggat gccgtacgtt agtaaaatct
    11581 cttggactag tttttcagat atcttgattg gaaccaagat gttaactgat agtccgcctg
    11641 cagacgcttt ttctattaca agatcgggag gtttttgatc gactctgata ccaattccgt
    11701 acagctcctt ttttataatt gccaaatggt gtggttggaa tacatcgaga acaattagga
    11761 ctaggtctgc acttcgagcg atagacagta cttttttgcc cagtcctttt ccggcggctg
    11821 ctccttctat aatcccaggc atatcaagaa tctgaatatt tgatcctcga taatgcatca
    11881 tcccgggaac agctgtaagg gttgtgaatt gatatgaagc aaccttagat ttagcatttg
    11941 tcaaccgatt aagaagagta gactttccaa cgctaggcaa tccgatgaga attacagtgg
    12001 catctccgcc ctttctgacg tcaaagcctg aagctcctcc tcctgttgat agtgttcttt
    12061 tctgtgtgtt ttcctccatg tctcgcctga gacgcgccat ttttgctttt aagagaccaa
    12121 catgatgttc cgtcgcttta ttgatctgtg ttttgtgaat ttcatcttcg atagctttta
    12181 tcttctctgg tattcccaat gtgtaaaaat acacaacaat tggactatta tcagtttagt
    12241 tttgcaaatg agccgaaacc atgtttttac tcgttagtta aatgttggtc taagggtctt
    12301 cctggaaaca atactttcaa tattgatacg gacaaagagg caggcggtaa cagaactata
    12361 tttcgttgac tttatcgata ggctaatatg ctaacatgcg agacctgcgg cagaatcaga
    12421 ttttactcat tgaactttac tttttcaaaa tctgtttgta aattggtcaa taatcaattc
    12481 attatggtat tgggactaat tgtcaaccaa tagagtcccg accttcttgt tctctatcct
    12541 catcagtatt taatttatga gaattcttcc gaaatccttt tttggacact tctgtccctg
    12601 tcagtctcaa atagagaaaa tccctggtga cagatagtcg taacttttgt gatacgaaag
    12661 agcagcagaa tctaaccaat acttaattac taccatagtt aaaatattga aagctaaaac
    12721 atgtcacctt cggaagctac tcttaataat atatctccat cgccaattat gcaaatgact
    12781 actggctctt gggtatctaa gaccttaatg actgcggtgg aattgggtgt ttttacaaaa
    12841 atttcaggtt atcaaaatga taacaatagt aaatctatga ctttgagaga atttcaaaac
    12901 ataataggaa ttgaagagag tagacaagca ttgatgatta tcaatggttg gtgattgtca
    12961 tcaactagag ttttcctcgt gaataatata agttatttta attctttcct gaaatgttta
    13021 ttaatttaga gttactacga gggacgtagt acccgcaaaa tatgcatttt ttgaaagctg
    13081 ctactttcaa actttcaacc tcacgaggtg gaggatgcct aaaataccac ttgacgatgg
    13141 ccatcaatga ctactactat aaaaacattt aaaggccaat aaaagttttt atatttgatt
    13201 ttgtgatttt tttgatgaag atcatcaact ttggctgatg atggccatta atttacttga
    13261 tgattgttat caactttttt ttctttttat ataattcaag gataatttat ctgtaaataa
    13321 ctaatagtga ttaattaatc gctgttgagc atcagtctaa gattcgggtc ctctgggact
    13381 atatcagtgc tttttagtgg gctgagtatc gcaaaagatt atggaatttt gtaagcattc
    13441 tatattaggt gtagtctaaa gtcttcgttc cttcaccacc aactgaatat tacatggtct
    13501 gattcagacg agataatgaa tgttaaatgg ttattgggaa aggcaaaaaa ggaaaatgaa
    13561 agaaatggaa tgaaaataaa cacttaatca acatatacat ttcattgttt gcagatagaa
    13621 ccaaattgta cgcataactg cgagtctcgt tgtcatctaa ctattcttgc tttacaccac
    13681 actttgtaca atatctcgag ttcggtttta ttttagatcc gcactctata cagaatttac
    13741 cattcttagg gttcgagtta tctgcacttg acggtataga ttttggcgcg gctgttcctt
    13801 cttcaggaaa gtcatacagc tttttaactg ccccagtggg tttgaacgtc tcattagtat
    13861 cgggcattaa ctcgaggaag gatgctttgt tttttcttag tttttcttca agatagagcg
    13921 tcagtcgtgg catagcgcct tcttttcctc cagggacatc tacttgttcc ccaagccaca
    13981 tagcaaattt cttcagagtc atctcgggag tagaaaaagt aagggaatga gtagacatgt
    14041 attcttcagt cgcggcccta gcattgaata ctttcatcaa ctgatgatat tcttttcgtg
    14101 tttttaatta ttgcgaattg ccacatttat tgcagaactt tgcctggtca ggaatagtgg
    14161 atccgcagac aatgcaattc ttaaccttcg cggactttgt cgggctgcta agtggtgtca
    14221 tttgggtaga gtcggccacg acgccagcag tttttcgaac gtctaccgag ccaactcctt
    14281 ccatgtcgcg gtccgtcttg gaaagagggg ggacagggct ctgtcctttt gagagatttg
    14341 tttggatcgc taatttgact tcgaataata cctggatagc aataaaattt aatgaagtca
    14401 tcccaatgag atactctgcc aacttggcta ggaaatcttt ttgattcacg tttcctttct
    14461 tataaagttg agttgcatcg atgaatgctt cgtactgttc ctgcgtgtcg tcggtgagct
    14521 gctttagttt ctctgctacg actctcaggc tgtcatcgtt tgcgccaacg gacattgctg
    14581 gaacagtttt tgtttgaggg actaataaaa gtttgaacct gaagtatcct tggaagaatt
    14641 caggtcaaac tgaaatccag tcacatacac accctgggtg accgccaata agatagttcg
    14701 gcgcccataa aagcccttct tggatgacgt ctagcatgtt ggactttttc ttatcgaacc
    14761 cgaattcgta aataattttt gtcgttacga gttgttaaca atcaaaaatc aaataacata
    14821 ttaatatcta tacgctcaag ttgacgactg gttgaaggtg ttttcagatg gagggatggt
    14881 ctagactggt tatgatacct gccttacacg cagggggccg tgggttcgaa tcccattccc
    14941 tccatagact taatcgcaca ctcatctgtg gttggtgaaa gccttgggcg cgataatatt
    15001 cgggttcaaa aggcttaaat ttgaataatt tttgacaaat attgaatgag tggttctgag
    15061 agtcttggaa gcgtaagtta cgagtgtatg cggtgcggga caccggtgac tgtggaggag
    15121 ttatccagat tgcctgaaat caaatgtatc tgcggcttcc gtgtatttcg taagtccaga
    15181 caaccaattg tgaaacaatt gaaggctatt tagtaagcgc ctgagaaatg taatttatag
    15241 tactatatcc acgaaatttg atatcatccg atacatgaga atgaaagctt cttatattat
    15301 ctcctagaat agaatacatg aaaattcgct gccccgattg taagaatgcg gcggaactct
    15361 ctgatgattt ttcctttgta aggtgttctg aatgttcgct tgatatgagt tacggagaat
    15421 atgtgaaata tgtggcatat aaagatgtac gataccgcga tatccttgat gattacaaac
    15481 caagaaattg aacatgggct tctcatgctg attgcagatg ctttttgatt gctctagtct
    15541 ttcccatctg agaaaccatc gtgttgcccg tctaactaac agtcgtaatc ttctaactcc
    15601 tcgggtcagg cttcagacat ttatgatggt ttatcagacc tgaaccagct gaatgaagaa
    15661 ttaaagcatt ttgattacgg gttactaatg acttctctgg ccagcgctga atacagcatg
    15721 tgtgtgagcc ctaaatattt ttcagtcttt ttaccactgc gcatcgttcc ttaggttttc
    15781 tttttaattg ccgagacaat ggacaatata tccctgtctt taatatgata gttgttcggc
    15841 aatctcaaac catcgcgtat atcaatagca taaagtaagc ctttaactaa ttctgtatga
    15901 atctccctcg caagatcctc aatagtagag cccgatttca ttaggtacac gtctggtaaa
    15961 atattcccgt gtttgtctga tagtttacct gtgtctgcga caggatatac tgcattcatt
    16021 cgtaggagct tgaacacagt tacgtttatg gcaaattgaa cgccggtccg catatattca
    16081 ccgagtatat cttttcggat gaagtttaaa gcccactttt gtttatcatt cagcttttgt
    16141 tggtctttta tttcaaatcg ctcgtctcct ggtagatatt taatcatacc gcgaatttct
    16201 gctcgtctta atgtgagttc agcatctgcg ctgcagggga cgattattgt atctttgtat
    16261 tcttctctta gcctcttgaa attttcagat gcataatgca gatctacctt attggcaaca
    16321 atcagggtgg gctttgaaat gtccctcaaa ctccatgaga aatctttagc ctttgctggt
    16381 tcccagtcat caaacttaac ccctagaagt ttattaacct cgagagcaaa tttgacgtgc
    16441 tgttctcgta ctccaatacc tctaaatatg tctgttatgg cggctacctc ctcaacccct
    16501 gaattgattg ctcttgaaat cttgtcacga ttaccctcta gaagcttgag gtaccacatt
    16561 accaactctt cttctatgtc agctatgtct gcgatgggat cgcccgtgcc aggttctgct
    16621 atttttcctg atgcatcaat acccccagaa atatctacta tatgtaaaag tgcatcggat
    16681 tgagctgcaa tcgaaagaaa ttggttgcct aacccctttc ccttccatgc accctttatt
    16741 aatccaggca agtcgactag ttcgatagga ataaaccgcc agccgtctaa gcaacgagaa
    16801 ttcttcggat tgtcctggac gtttaattct ttgtgaacgc acagagtgat tgcattagcg
    16861 ttcccgatgc ttggctgctg ggttgtaaac ggataattgg aaatctctcc tgttccaagc
    16921 gtagcagaat taaaaaaggt agtctttccg gtgttcgtct tgcctattaa ccctatcttt
    16981 atcactaaca acgaatcagt cgatgctaat ttattcttga ctaatgtgcc attatggtta
    17041 gcattacgag tacattctgt ggtggtatga acacgcattg aacagctctc ttctttgcct
    17101 agatattgga atgcgcgaga taacataatt aattaattaa ttaattaggt ccacgttcac
    17161 ccagttatac gccaatcctt tccaataatt caaacacttc attactagtc ggtggttccg
    17221 cattttggct tctcaggtag agtgaaacag tcgcattagc gaatgtcaat cgctctgtgg
    17281 cattcaagcc agcaaggtag ccgagtatgt cagcagaatc ccaggaatca cctgccccag
    17341 tcaatgtcct cacgtcaact ttgattgcac ggacaaagct ggtttccttt ccattcgacc
    17401 atgctgctcc ttttctagta tgcaagtcga cgctgatatc aattttttct gcaagctgcc
    17461 tgacagcatt ttttatatga tctacaatat cttcacgagt aagttcaaga tgttcgagac
    17521 ccgtaacttc tgcaagggaa ttatattcgc tctcatttat actcagaaca tcggtgaatt
    17581 ctgcaatata cttaagtgag tctctaaagt cttccttcct agtttcgata tctgctgggt
    17641 caataaagtg aagagacttg ggagaatttt taaacgcatg ttcagccaat tgtgttccct
    17701 ttagattgct gccccagttg acgactaccg tagcatcaga atttttcaaa taattcaggt
    17761 gttctgaaga attaaccatg tctgggccaa atttactgtt gtctccaaca tcgcttatca
    17821 ttatattgac cctgacatct ctttcgttaa gcagttcaaa tgcggtggta ctgccgtgtc
    17881 ttccgcttgc aaccaagagg ttcacgctct ctccgaatct cgaaaaagtc tgtctaagga
    17941 tagctgctcc gatatcatct gccatagtaa ataaagcgac ccttacacct agtttggcta
    18001 ggcaatatgc aacatttgcc gcatttcctc ccttgatatc ggcagttgga acacctcgta
    18061 tgctgccacc accgagcatg gctttgtcat taatgttgtt aaagaattcc tctttcgatc
    18121 ctaactttat aacgcggtca acgaaaaaat caggcattac aacaatggga ttcatatagg
    18181 agtagtaagc ctttattttt tctgataaag gagatctaat tttctgacac ccaaacagtc
    18241 agaatcctgc tactctttta tgtatatacc ttaagtcgtg acaaaccaga cgtgtcgcgt
    18301 ttaccttcgt acttaaatct ttttcttgag gatacttgct ttttaagcag atgctcgctt
    18361 aatattggta catactgatg gatgaatgaa cccgccacca ccacctccaa tagatgcagc
    18421 agagttcgac gctcacaaag acctgagcgc aataatcatc cagttcttat gtagaaacaa
    18481 agaaaaaggt ttttcagcga aggagattgc agaagcagta gggctaaagg aagaagacgt
    18541 aaataactcg atggtgaagt taggtctgac cgatcttttt agtgggctta ccggcggcat
    18601 gatttcgcgc aaacgtcgtt cgaattcaaa gaacaaggaa gtggttatta agatagaaga
    18661 tgttacaatc agcggagtaa tctactaccg atgcgttgag aaagccattt aattatttga
    18721 acgatatgaa ctggaaggca cgatgcgacc tagtattgct ctaaagaaag aaaaaaataa
    18781 atgctgtgta ttgtgctcaa ctaaacgtaa agtagggtta tgaaataggt gagcgcctgt
    18841 ggcattttta ttcatccatg ccacccatgc cacccatgcn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19141 nnnccatgcc acccatgcca cccatgccac caccaggtgg tccggcaggc attttagact
    19201 tgcttgctgc gatgacatca tctatgcgta agagcatagt ggccgcttct gtggcagatt
    19261 ttatgatctg ttccttgaca accagcggct caaggacgtt cagcttgtac atgtcggcta
    19321 caacaccgcc tcgcacgttg atacctgtcc atttagctcc cttcgattgt tttgcacgca
    19381 gctcagtcat ggtatcaatt ggatccatac ctgcgttctc agcaagggca agtggaattc
    19441 catcaagcgc atctgcgaat ttctgaactg caagctgtgc acgtccttct agattgcttg
    19501 accactctct caactgactc gcgacatacg cttcgggagc acccccgcca gcgactatcg
    19561 caggtttctc aactacatcc ttagttacca tcagagcatc atgcaaggag cggtctgcct
    19621 catccactac cctttgtgat ccgcccctaa ccagaattgt aactgctttg ggattcttgc
    19681 acccttctat aaagacccat ttgtcagttt ccaccttacg ctcttcaacc aactccgcag
    19741 aacccaaatc cttagtgaca aggtcatcta gattatttac tatcctcgca ccagttgcct
    19801 tcgataattt gtacatatcg ctctctttga cgcgccttac tccaagcatg ccttccttag
    19861 caagataatg ctgcgcaata tcgtcgatgc ccttctgaca tagtacgaca ttggcgttag
    19921 cagacttgat tttttcgatc atggatttga gcatcctgtt ctcttcatca aggaacattt
    19981 gcatctgctg agggtcatta atacggatct cagcactcat ctctgtcttt tcaatttcaa
    20041 gagcagaagt tatcagcgct atctttgcat tttcgactct cttcggcata cctgaatgaa
    20101 caacttcctt gtcaagcaca atccctttga tgagcttggt gtcacgcata gagccacctg
    20161 ctttcttttc cacctttata ttatctaaat ctacctttag ggcatcattt ccgccctcct
    20221 cggcgatctg cttaatagca ttgaccacga tttgactcaa agtggggctg tcttcagaaa
    20281 caagcttgga atccatgctt gtccgcgcga ttcttatcat tacatctttg tcatctacgt
    20341 ctactttaac tgcaattttt tgtaataact tcaatgcttg ctcagccgct gctgtataac
    20401 cgtctacaat tacagatggg tgaacatcct tgcttatcag atcctcagct ttttccaaca
    20461 aggcacctgc gaaaatgact gacgaagttg ttccatcgcc aacttcattg tcaacagatt
    20521 tcgacacttc gaccatcatc ttggcagcag gatgctgtac atctatttcc ttcagaatag
    20581 tcgcaccgtc atttgtaatt gttacatcgc ccagcgtgtc gaccagcatc ttgtccatgc
    20641 ctcgcgggcc cagactggtc ttcacgattt ctgaaatcaa ttttgcggcc gaaatgttgt
    20701 ttttttgtgc atccttacct ttattttctc ttgttccttc ctttaaaatt attactggca
    20761 ttccgccagc cgtggtttga attgaagcca tatccatttt cctctagtag aatgttagca
    20821 ctttttctca tgcccctttt atagtttttc acatcataac tgagccaact gcttttgctt
    20881 gttgctgaat ttatctctaa agctagtcac gtttatgata aatctctcat gttttccatc
    20941 gccgattctt tcgaggttat cagacgcctc aacgtcaaat aagactcttc ttacatcagc
    21001 cgatacaacc ttcgccctga aaactacact agcacccaca ggagtggctg ctaaatgtcg
    21061 aatgtttaca agagttccaa cagaatccca atctctaagg tggataaact gctcctttag
    21121 cagtaacctg caagtttctt ccatctcgga tatcatcgaa ggtgttgaaa gtacgttttc
    21181 tccttcccac agaaaacttg tcgtttgagc agaactaatc ctaatcgttc tctcttttgt
    21241 tgaaccaact tttactgcaa aattcatcta acatacgcaa gacagataaa aaatttaaat
    21301 tttgtcaaat ctatcagatg aaaactgtct ctcatttcat tcctgttttg ctatgcctac
    21361 gcgcatctgc ctttctctca ttttcttccc gcacgtgatg cagcctgtct ttttcatgca
    21421 agaactcctt gccgcaatta tgacacttcg aaacagattg gtgatgcaca tttttcagat
    21481 gctctattag atcgtctagt ttattgaact tttcatgaca gtgttcacat tgctttcttt
    21541 caggaataaa caggatcata cttgctattt tattcagttc gcctcctatt aagcatctgc
    21601 ccttagacat ataatattca gctata
//
LOCUS       DAOR01000298            4299 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_88260, whole genome shotgun sequence.
ACCESSION   DAOR01000298 DAOR01000000
VERSION     DAOR01000298.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_88260"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1287 a          851 c          927 g         1234 t
ORIGIN      
        1 gcaggtaatg cgacaaaagc agcaagcaac gcaacctctg gaaattcatc aaatccgctg
       61 gcgaagatcg gacaagcatt atctgggcta ttcggcggta aaaagtaaaa aacaagaaga
      121 gtcttgttgc tatatgtcaa tatagtatac aagacctaat ttttctttac tcagcatact
      181 tcatcaagtt tgcttgtagc aatctttgga gatcgaattt tctatgttta ctttgaagct
      241 atgagaaagg gatgtcacag aaaccaattt taatagaaaa tacattatca tgtgttgatg
      301 gcctcagaag aagaaaagta tatcacttat gatgaatttt caaagatgca tctgaaaatt
      361 ggcaaagtaa ttcatgccga aactattccg ggcatgaaga aagtctttaa agtcatcgta
      421 gatattggta acgaacgaag agatctagcc gtaggtgcag caccttatct ttcccccgag
      481 caattcgttg ggagaatagt ggtagtttgc acaaacttgg aacctcgtag gattggtagc
      541 gtgacttcaa atggtatgtt gctcgccgcg gagggaccag atggtcggcc cgcatttctt
      601 acaatcgccg acgatgctcc tcttgggtca gctgttcgtt agattagatt ggatttattt
      661 atttatttga tcgtcaacca attcctggac gaaaattagg tggctccttc tgtttttgaa
      721 tatcttctaa aattgactcg tattgtttat caagcccttt ggtatctagt tgcatatttt
      781 caattttagc aaatgccttc aaaatactct ttgcggcgag cggttgggga gttgttgtct
      841 cggaaatttc accaaataac cctattgctt caatgtctct atctgcagcc atgcccaaaa
      901 ttaatccgtt gaaccatgtt atagtgttta tttcctggcc tagtatgtcg atacctgaat
      961 ttagcagtat ttgttttagt ccaggattgc ttgcgactcc acacaccatc ggagcacctg
     1021 ttagatgttg gcggagatat ccccctgcgc tgtataaacg cctaattttg ccaattgtct
     1081 gaatatgatc aaggaagctt ttgcataatt gatagagttc ggttgggtcc tgtggctgtg
     1141 aatttcctgt gaaaataagt agattatggt ctgtgttata gtaaagttgg taatgatcac
     1201 taacggtttt cacaacaccg tcgatatacg atacccatgg cttttcattg gaaactattt
     1261 cggcaatgca ttctgatttc aagtttttcg ctaaatatgc tgacacaata ccacccactt
     1321 ttcccatatc tgggagtgat gcgaatacgt tgaatccatc tgttttggac tgtttgatat
     1381 tatctaggcg catcactagt ttccattcca tttattttca ttaaaaaagc cgcgatagca
     1441 gtatagtctt gttcggagta tccgctattg tttgctgttc tatacatttg ttgcaccaac
     1501 gtagtcaatg gcaacgaaac ccccgcagtc tgtgcactaa gcgctgccag atcaaggtcc
     1561 tttaacatat ttctaagata gaatgacggc tcgaagttat ttgctatcat cttcgggccc
     1621 tttgcttcgc tcagttgtgt tcgaatagat gtggaattca atatctcaat aaacatcttg
     1681 ggatcgattc cggcacctct gacaagggca ataccttcag acatcgcccc agctattagg
     1741 ccaatattta gattcaatgc taacttgaga atgctggcat cgccatctcg ttcgcctaga
     1801 tagaacattg cttttgagat gtttttaata ataggtgcta ctttctcaaa agcctctctg
     1861 cttccgcaaa ttatagggac caattcacca ctttcagctg cagtagtccc tcccataaca
     1921 ggcatcccga gcattgcaat attggcctct ctaaaacgct gcgcgcaaaa ctttgattct
     1981 tcgggagata ttgtggtagt gtctgccacg ataaaatctt ttgaattacc ggcaagtatt
     2041 ccttcatttc caaaacatat ctccttcact gcaataaaat ttgtaacaga aataagaata
     2101 agatcagaat cctgcgctag ttccttaggc gtgctagcta cagcgactcc cctgtttgcg
     2161 aagggtcccg tctttatgct atgacgatta taaacagtaa tgagatggcc agtatcgata
     2221 agtctgcgcg ctatcgcagc tcccataagt cccagaccag ccagtccaat tttcatcaaa
     2281 ccaaattaca tcaagatatc ctacgatatt aatcatagcg tttctcacaa agttgtccat
     2341 atacaatatt atgctgtatg aatcctgatg catagttaat aaggctgggc ttgatatatc
     2401 ggggagacag cagcttttat agcgccaaag tttactgact caggattgcg gagtgtgtct
     2461 gtgagtaagg gcctagtaat cgtttacacc gggaaaggca agggcaagac gacggctgct
     2521 ttaggaatcg ttttacgttc cgtgggccat gggcatagag tcataatgat tcagttcatc
     2581 aagggagaat ggtattatgg tgaactaacg agctcaaaaa gactagagcc cgaattcgaa
     2641 atgatcgctg caggtaaggg atttgttgga attttggatg atgaccacac tttagagcaa
     2701 catcaagatg cggcgctgaa agcaacggcc ctggcgatag agaaactttc aactggcctt
     2761 tacgacgtaa tgatactgga tgaaatcaac tatgcggtaa aactaaactt gatatcagtt
     2821 caaaacgtgt tagaaattgt aagaattaag ccttccaaaa ccacgctagt tctcaccggt
     2881 aactatgcgc ctgtagaggt catcgcattg gcagatctag ttacagaaat gaaagaggtt
     2941 aagcatcctt atgggttggg aaccaaggca attaagggaa ttgattattg attggcagtt
     3001 aactcatgaa atcatgatat ggtaacctta aagtatgcca tttggaccgt ttacctttat
     3061 aaatggtgac tgccgaaatc cgacggcttc cagatgaagg cgatgttatt atcgctacca
     3121 ttaggcaact aacaggacat ggagcgtatg tcgttttgga tgaatacaac ggcatgacag
     3181 gcttcctgca tatttctgaa atcgcgaccg gctggatacg caatctcgag cgctatgtta
     3241 aacctaggca gaaggcagtc ctcaaagtta tcagggtcaa taaaaccaga gcggaagttg
     3301 atttgtctct caagcaggta tccggggaag agagaaaatc aaagctcatt gaagttaaga
     3361 agaacgagaa ggcttccgca tccatggata ttatcaagga aaaaacaaag attaatgatg
     3421 ctcagctaaa agaaatagag gataaaattc ttcagaaata cgactacgcg tacgactttc
     3481 ttgaggaagt cgcaaagaaa ggaatcggtg caatcgcaaa tctagattta tcgcaggatg
     3541 tcttgaaagc aatcgaagaa gagggcaaga aaatacagat tccccacgtt gaaatacgag
     3601 ggatcgcgga gatcgctgtg agaaaaccta atggaattga tgtaatcaaa tctacactac
     3661 ttccagtaga gactagcaag agtgctacta gtactgtgag cgtgacctat attggagctc
     3721 cgcgatacag aatcgtagtc gaagcggaga attttaaaat tgccgaaaaa gcaatgaata
     3781 atcttgtaga aaaaattcat tctaatatag ataaacatca tggaaccttc aattttatcc
     3841 gtgaagagtc aaagaagacg catcttcaaa ggtaggtaat gttggcagat gaggcatctt
     3901 atccgaaaat gtccaaaatg cgcttcatat actcttaaat acagttgcga aaaatgtaag
     3961 gtcccaacga tcgatgctca tccggccaaa tattcacctg acgataaata tgctcgatat
     4021 cgtatcgctg ataggtatat ccaaacaaaa gaagtgagtg attcggagcc tctaaaagtg
     4081 gatcatgaga acagtcttta atagcatcca ctaaaaattt gttcagcaat ggatgttgct
     4141 tatcgtaatg cgtgtggtga gctgcgtcta gtgttttaaa gcagtagtat tctttgttgc
     4201 atttataata ttgtgtgcgg caaagctagt cgcattagta tttcgtgtaa tattcagaac
     4261 ggacgcggca gcagcagtag cattcattcc agcactctt
//
LOCUS       DAOR01000299            4952 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_87345, whole genome shotgun sequence.
ACCESSION   DAOR01000299 DAOR01000000
VERSION     DAOR01000299.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_87345"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    689..761
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1665 a          793 c          964 g         1457 t
ORIGIN      
        1 cggaagaaaa catgtatttg taatacaaca tgaactaggt gagaattggt cactctttac
       61 aaaagagtta ctaaaattga tatttgaaaa cctagctaaa gttagagctg aagttaatgt
      121 tactgcaaat atgactgtag ctgaagttat tctttaatta cagaagaatt ttgataatta
      181 tctagaaata tgttcaggta acatttatga aaatcataaa attcttagtg cagtgcatac
      241 atactagtta agtagcatca cgctgtttgc cttacttaag gtctgttaga attatgatga
      301 ctcacgtttc gaaatctaat cataataaaa ggggacgagt aaaaccactt actgacgaag
      361 atatagtaca cgaatacttt aggctcatta aaactaaaga tatgtacaga ttactcgacc
      421 tttttgcaga tgatgccgta atcgacgaac catttagcaa atcagaagat ggtaaaggag
      481 gattacgagg aaagagtgga atagagcctt ttcttaacgt ggcaatgatg gttaatgatg
      541 ggttgagaga agaaatcgag ctcgaaaaat caaaaccgag aagcgataat ggcatcaaga
      601 gagttattgc atatgtgact tttgagagag gaggaaaagt aaaagccaga ttcacatttg
      661 aattaagttc agaagagaat tacaattcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttcagaaga gaattacaat
      781 tctcaaaagc agaaaaagat tcagtctttg cgtattcagt ttgttgagta aatctatatc
      841 ataagagaaa aagacttaat gaaacaaaag cagatacagc tcttaatcgc tgattccatt
      901 ggattacaag tggtagctaa tattataatt gaacaaagtt cgtatacacg cgtatttcaa
      961 tatttaaaaa atgtgcgcag cacactattg tccccatatt ccttttctaa tcaagcggga
     1021 ataaagtggg agatatatga catacttttt cttgctttct tgattatcat ttcgtttgca
     1081 ttcctggaac atttttgcgt gcgcaagtag gtataatgca gtataatgta tgcagtataa
     1141 tgcatgcagt gtaatgcact gaattcgttg tgattataat catgctccat gttgtattat
     1201 tttaagaacg gagacgagaa tgagttgtaa aaacatctgt acaagataca aggcaactaa
     1261 acatagaaga agtagttact atgcacaagg ttacaaaaga tgttctatgt gcgatatttt
     1321 catagaatgg gctggtacaa gatgtccatg ctgtgatcta gtactgagaa cagtcgccca
     1381 tcagaagaca aaagagttcc gactttgaat gtgaagatat ttgaagggct tttagacacg
     1441 gatagctgac gccaatttac atctgtctct ctacattcag tatctgtact atctctcggg
     1501 taactgcttc agatagtcta tctttttctg tgtgtatgcc tgttgtctac atagacaatt
     1561 ctgagccatc tattggagcc aaaattttgg agaacttctt gtcttctgcc tgatacgtaa
     1621 atatattatt cgaataacag gtttttaaaa agtccgaatg cagtgcattg cacaatattc
     1681 caatctataa tataccatta tttacatagc aatatcaggg aaatgagatg agtagtatag
     1741 aaaccctttc agtatctagt tttatgacta gagatgtaaa aacggaaaaa gaagaccaaa
     1801 atgttctaac agcttgtaga ataatgcatg aaaataatat tggaagtgtt attattgtaa
     1861 agaaagacga caaaaataat atcaaaccag ttggtataat cacagaaaga gatgttgtac
     1921 gcctgttagg atcgttaaat ccatccttgt tacagactcc actacgtgac ataatgagta
     1981 aaccattgat taatgtctca ataaatagtt ccgttagaga cgcaatccaa accatgcagc
     2041 aaaagaatat tcgccgactt gtaatatctg atggtgaaag gatgattggt ataatcacag
     2101 ctaaggacgt attcagagca ataatgaata atcaagatct tatacctagt ttgctaggtg
     2161 ataaactgct aatagaagat aaaacaatat atgaccaatt tggtcaggat tggttttcgg
     2221 atattttaca gaaaccataa gcaaacaaag ggggtagtag tattagtaaa aaaaacaatc
     2281 aacgattaat taagatgggg cggtaataac aatagtgatt tggagttttg tatgaaccac
     2341 ctactagata ttcaaataaa agacgcagct ccccatatct ttagacggcc atttttgttt
     2401 gttgaaccaa atacacgaat gctgcaaata gcaacctttc ttgcaatagg ccctgaaatc
     2461 catattgatg gattaatagt aatcgatgat aaagtacaaa gaggaagccc cataggtagg
     2521 attagtagta aacatattat ttctatcatt ttagactttg gctatccaga ctggttgcaa
     2581 aaaaacgcat cacaaataat ggatagcttt gtaatgcctt tagaaatgga ttctcctctg
     2641 aaaagagcat tggaagtctt tgagacaaca cggtttgctt ttattcccat actagcaaaa
     2701 aaggaagaaa ggttagagga cgaagaaggc gattcattaa tagtagtaac agcatcactt
     2761 gcaatacgag atattctacc gctaattgca aaagctaatc ttagcatacc aataaaggaa
     2821 ctctcttctc aattaatatc cgtagatggt aagactagcg tcagaaatgt attagactac
     2881 atgatgagta agagtattag aaatatagga ataaaggaag gactctctgg tcatcatggc
     2941 gatggtgttg gcggtagcga aggcagtaga aaatctaaat tacttcgtgt tataaacgat
     3001 agaaagatac tagagtttct attaagtcat aatggaagag agattatgca caagaatggt
     3061 atcgcaggct tagcagacat taatatcatc aataatgtgg atatgatatc tttaaccgag
     3121 gttaaatgcg attctactgc aagcaatgca gcagaacttt taatggatat aatcaatcca
     3181 tgcttgattt tggaaaagca aggagaaaga gagccgcata cttctatagt tactccttgg
     3241 gatattgtca tgaagacatt aaagtcagat gactgtgtca cctgatgatc ggcaagaaat
     3301 aatcatggca gttaatgatg tcgctagcta agaataactt atagtgggat taatttcaag
     3361 ctgttttatc tcttagcgac aactaaaaaa atcaatgggt aaataaatat ttcagtttta
     3421 cttttttcta cctatcgaaa ttgcatgaag tactgattga gtcgattctg gtcttctggt
     3481 acgtgaaaga tacatggcta aagcagttac ggttactatt cttgtcagta gattcgttaa
     3541 ctattggaag cctacggatt ttgtttttgc tgcttcttcc actgtagctg taagttttgc
     3601 agtgataagc ggatgtgaag cgaaactttc aagcaatgta gcacgaagac agatgtcttc
     3661 tttctttgct agtcctctca ctagatctct ttctgttatt attccaaatg gtctaccacg
     3721 tttagttaca ataatgcttc ccactttctt aaatgagcgt aaaatataac ttagaagata
     3781 aactgaaaat tattatgatt tagaagatta aactgctaac ggttaagtct taggacttgc
     3841 cgctcttgtt aataacgtac cattacccat tagtcatatg tggacttggt ttgcttgcac
     3901 agccttcggg gtgcggcgga atagcagaca tgggtaagat tcagtccgtt ggacaattcc
     3961 agttccttac aaaggcgctc agacgacgat gaacactttg attctaagct caacgttatc
     4021 tttctgctat tgttggctcg catatgagcc ccaaaatcct gatatttgtg taaccaattt
     4081 cggaccaaat gaagagcccg gaaatacggt aacgatactc tccagcagcg aactctgcga
     4141 atctcaagaa ctggaaataa aatctttatt agaatcggct cgcacctaat catggatcca
     4201 ctgctatgga ttatgaagga cacatgtgaa gacaaaaaca agtaggctct tgttctttca
     4261 gtaatcgtta gtatgctttg tccaatcata aaatgatact gacagtagaa gaatattgtc
     4321 aacaaggtat tacattgtgt caatgtatct atattcacaa gctttatgat ctggcaactg
     4381 acctatgtta tcatgatttc aattagtgat tgcattactt acaggggtgc aggggtggag
     4441 acacggatgc tgatctttct actggtttct taaatgaaga agaaaaaaca gttacatgcc
     4501 cagtgcattt gtttattttc aaattgtatg atggtgtgtc acaaaatccg cctgctgaga
     4561 agccgctaaa aacgtacaat gttaaaattg agaataaggg cgtcagaaag atagtggaat
     4621 gacaggttcc ttgattctag gcgataaagg tttaaggaac tttgtcttga catttttgtc
     4681 cacaatcaat tttataaact tagtataagc atcttccaat attgaaaaag caaacgttgc
     4741 agtagctata ccaaaaaaca ggaccataac atttctttac cttggaagag acggaagatt
     4801 agacaaaata tcaagtaaca gatttcatcg ttggagatgt gaccctccgt ataacattga
     4861 aactgccaaa gagatgtatg ataccgacct accttcgtct aagagattac ttgaagcagg
     4921 tgctagagtt tcttgggcct cggaattatc aa
//
LOCUS       DAOR01000300            4786 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_15678, whole genome shotgun sequence.
ACCESSION   DAOR01000300 DAOR01000000
VERSION     DAOR01000300.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_15678"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    235..490
                     /estimated_length=256
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1168..1339
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1418 a          834 c          771 g         1335 t
ORIGIN      
        1 attgagaaag ttttgtaagg atatatgcag actctaaagc tcgcaagacg aaaaattcag
       61 aaagttatta taacatcgat ttcaagcgtt gtgtagaatg tgagatattt ttggaatgtg
      121 aaagtatgag atgtccatgt tgtgatcatg ttctaagagc aaggccgtac agcaatgtat
      181 ccaaagcaaa attgctgcaa agtctttact atgtctcgtg atcacagaag acatnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn acaatattgt tgtagaatat ataatctagt tcaaacttat gctgggtgac
      541 tgtcggatta ttcttatcta ttcttttcac gacgacgatc acagtatagg gggcccctat
      601 catcattaaa tccctccctc ttcagctggc atttcactgt atattgcaag ctatgaatta
      661 gacattacaa aagaaaaagt tgcaaaaatt attaaccacg caaatgccaa taaggaaatt
      721 agacgccact cgcaatatca taaatgaggt tcttttagtt aggtaatggt cggttcaaaa
      781 ggaaaaggat aagtagaatt aataagttac aaggcaatgc gattaaatca attgaagaaa
      841 aaattaacaa atgtcctttt agtgatagca tcgttagttt tattgatcac tcctatagta
      901 tcggtagcag cagcggctac tactacctcg actaccattt caacagccac acctatcaaa
      961 catcttgtcg tgatatttca agaaaacgtt gcattcgacc actattttgc tacttatcct
     1021 aatgctgcga atccccctag cgaaccagcc ttttcacatt ctccttacac accttcaatc
     1081 aatggtctgg ctgcaggtct gttgactaat aatacaaatt tggcaaaccc attccgcttg
     1141 gatagatctc aagctgtaat agtaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnna tctcaagctg taatagtaac ctcatgtaat cctgcccata
     1381 aatacacact acttcagaaa tcttttgatg gtggtcttat ggacaaattc gttcagaact
     1441 ctggattatc ctcacagaaa aattgtgatc ctaaactggt aatgggctac tttgatggta
     1501 atacggttaa agcactctgg aattatgccc aacactttgc aatgagtgat aacttttata
     1561 gctccaatat tgatccatcc cttccaggcc atctgaagat catctcgggt caaacccatg
     1621 gcgcgatgcc aactaacatg tcaggtaatg tggctaacgg tactgtaata ggcgatatac
     1681 cttcagttta cgatgattgt tctgtaggta cgaaaatatc aatgacagga aagaacatag
     1741 gcgatttaat gaatgaaaaa ggtatcggct ggggatggtt ccaaggaggt ttcaaaccat
     1801 ctggtactac ttccaataac aaggctattt gtggtaccgc acatgtgaac atagcaggca
     1861 agaacattac cgactatgta gtacatcacg aaccatttca gtattatcga tcaactgcaa
     1921 acccacatca cttggctcca acttcagctg caatggttgg aaaaacagac gacgctaatc
     1981 atcatgctaa gaacctattt cttgatcctt atacaggttt agggaaatga taatctatat
     2041 gctatgaaaa atgacaatgc tagtcaacag taaatccatc cctccccaat cagtatttca
     2101 ctctatatgg ttattagccg caaggtcgtc aacagcatcc acctttattt tcgtcctata
     2161 tggcacttta gagtcttgtc cagggggtcc agttaggttc tgaataactt tacaaccctt
     2221 gttatcatga agttaatcaa tacaagcgat attgttgatg cagctaatga gccaagcata
     2281 gatgcttgat atgacagtac gccaagttgt agaaactgat aggtgagtaa tacctttgat
     2341 aatgaatttc agaaatagaa aaggcagatc gctctttgta ttaccttaac gttagcccag
     2401 ctccctatgt tcctctctac aaatccgaca tgtaggcgct atctataggc atagactgat
     2461 ctcttctttc cagcgtttaa atgtgttgat aatcctagcc tcttcattga actatagcta
     2521 gaacatgcaa tttgcttatg cgatgttcga ccactcataa aataatattt caatgaatca
     2581 tcgactaatg ataacgcact aaagatacct gcgacaacac taccagccat cgttgctatc
     2641 aacctctttc ttctgggtac aagtcgggta gcaaaaaagt tactacaata ggagctaatg
     2701 atattggaaa tagtactagg aaaacttatc acaagagata tactaatcca gactagttca
     2761 ttaataagaa actgcttgtt atgtgaactc cacactttgt catccttgta attcactgtt
     2821 ctactagatg atattaacgg cttgattaac cttgtaaatt aacaatgtta actcacatgg
     2881 tttagcatgt cttaactgtc tcctattgct aaaagtagtt ggtgatgtag atatgaaatt
     2941 ggaaggattg actaagttgg gtagactgac taagttgggt agaatagaat tagggttaat
     3001 cggaggcatt gtgctagtgg tggtgatcat gagctatttg atattctcaa tacagggtcc
     3061 tacaattcgt ttgactccat aattttattt ttctttcaat ttaccaacaa acaaatcttg
     3121 ttatctgtat acaccgctct tgattagacg acgtaatatc tcttacgctg tttacaaacc
     3181 atgttaacca tgagtaatta ataaaatggg tgcagcgcat tccatttttt ctgatatact
     3241 ttatgctaaa gtagtacagc tcatctacaa gattgaaaat ggcgttgtta attatagtag
     3301 tgtattcgtc gaatatacga tgttattttt atactcacac actgttaatc tcattgatga
     3361 atccaaaaac aagcattgta ttcagcattg catcaatcgc cgcggtagtg ttactgtttg
     3421 cctcaggttc tatagttgga aaccagctag catttgcaga tggacatcat taccaccacc
     3481 accattagaa ctagaacaac ataacttcaa taactgaaag acattggtac ttgaaaagac
     3541 caactaccgc tttttttatt tttttgatga gatgggatgg ggaaatggat tcgcttaact
     3601 gggagaaccc gttaggatta gtggcgttaa tttatgaaaa ctaaatcgat attatccatg
     3661 ttagtattga ttgtgatcat atcttatgtg tatccaactg tagcaactaa gcaaacagta
     3721 tttgcacaac aggcaacaaa accaacaaca aatttcttgc catatgtaaa ttccacgtac
     3781 ggcataaaac tacaatatcc ttccagttgg gataaggaag aaaacggtac aagacaggat
     3841 acagaaactg acgttgttac attctctcct ccagttgtaa actccaatgc aagtctcgat
     3901 atatcgatag ataacatttc agatgaaaaa ggtatacctt tagctcaata tgcaagtgac
     3961 agtatcagcg atttaaaaca atctcttaag gatttcaaat tggatggatc aactacaaat
     4021 aacatacttg ctggattacc tgcatacaaa tcgatatata cctatgtagg tgaaaacact
     4081 attttcaaag atatggaaat tggagtaata aaaggcgata aggtatatat tctaacatac
     4141 gaaggagcaa taaatgaata tgataagtat ctgccaatta tacaggaatt gattaattca
     4201 tttcaactag caaaatgaaa cgctgctgca gaaacaggaa tttgaaatga agaacatgaa
     4261 tatggcgact aatatggata ttatcgtatt ctatgattta atagcgaaat tcgaaatatt
     4321 ggatatagca gctttcgttt cataaggtag gaagcgcatt tctattagcc aagtatgaaa
     4381 aacaatcgct gtgacctcag atcccccatc gctttatcat acaagaaata taccgtaaaa
     4441 agtatattgt acattcgacg aatgtactgc aaaagtaata ataatcatat tttcttatat
     4501 ccttctagtg gctaatcaga ccgcacattc aatgttgaag ctatttatgc cattttactc
     4561 tctcatcatt tataggtcat accttgcttt gcaatcagct tcggttaaga tacaaagagt
     4621 catggttagc aaacccaagc tatttctaat aatatcagtg ttgactattg ctgtactact
     4681 accatcagct attcttccgt atattaacca cccggtcttc gtttaccgga tttctctcca
     4741 tgtagccagt atcataatat ctgtttttct tattgtagtc tcgatt
//
LOCUS       DAOR01000301            3893 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35138, whole genome shotgun sequence.
ACCESSION   DAOR01000301 DAOR01000000
VERSION     DAOR01000301.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35138"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1040 a          795 c          734 g         1324 t
ORIGIN      
        1 ttattgactc atttattgac tcataccaat catgtttcta tgtattaccg gatggtgagg
       61 cagcgggtgc acaatttttc acgataccga tgggatgaaa agtttactca tctatttact
      121 gatgttagta gcaagctttc tttgtggtaa gtttcatcta ttctagtgta ggatttgcag
      181 aaggtagcaa tcctcgttct agcaagtatc aattaattta caaataccac ctcgtcgcag
      241 aaagcttgcg gaatggcctg aagtgtatga aagaccgggc agcacattaa tgtatacttt
      301 ttctcctgct tcaatggtag ttgtcttttc aggtaatagt aggtaaccat ttgctttact
      361 caaaacagtc atcattgctg tctcacctgt tatgggcgtt gccttaacat taccctcttt
      421 tgataaccag gcgttaacgt aagctatttt tgtgaaattc ttaaattttt tgcgtgcctc
      481 ccacttttct gttaatatcc ccgctattct cagtccatta aattctggca gacctgacag
      541 atattgtagt aatggctgag caaatgcaat aaaagcgtta acagctcctt gtataggccc
      601 aggtaggata attaccggtt taccctttaa cgctccgaca ccggccactc gtccacgatc
      661 aagcttcaca ccatgagcta agacaccagg tcttcctaga gagttgattg attcttccac
      721 aacatcgtat tctcccactg aagaacctgc tgttgtaaga atcatatcag atttctctaa
      781 agcgaatctg atcttctttc tgaccttgtt gacattgtct ggcgttacac ccaaatcaaa
      841 ggataccccg ccaccttcct gtaccaatct agaaatgaca tggctgttgg tattcagaat
      901 cttacccctc ttaatttcat ttagattatc agttagctcg tctcctgttg gaattattgc
      961 aactctaggt ttcataaata ctggcactct agtaatacga accatagcca ataacgcaac
     1021 atcttgtcct cttagtatat ttcctctgtt cagtaacagt gaatcccgcc gtacgtcctt
     1081 acccaaagga gagacgaaag ctccctttgg aagcgaggcc agaattccaa ccttatcttt
     1141 cgattcatgt gtgtattcaa caggtacaac ggtatctgcc ccttgtggca aattgccacc
     1201 agtagatatt ctacatgctt gcccagaatg caagacacca ctcggaacat tccctaatcc
     1261 gacatttctg actattttta acgttattgg accggaatca gaagcatgtt taatatcatt
     1321 agccaacacc gcaaacccat ccatgtgaga agaatcatac acagggatat tgacatctga
     1381 tattacatct tcagacagaa ctcgtccgta agattccaga actgaaataa gctcggcttg
     1441 tggttggacc tcaatgtttt gtctaaggct cttgtaggct gctccgatcg aaagatatgt
     1501 aataggtttg agtgtcatga aagaattcaa aatgtagtct acaacaagta ataatatctt
     1561 ttgccatttc tcataacagt catcgatgaa atatttcata tttggggtac attgacaaat
     1621 ccggaaagat ttactcgcaa atattcaaaa acttggtcag taattatatc agctgcttgc
     1681 tggtgtgtgc tgaacaatta tacaaagaaa aatgaaaaca aaaaaattat tgtttgtgaa
     1741 ggaactgttg ctgttgtaca gtaaggaatg aattccaagc ctttgcggcg gtggttgtaa
     1801 attctgtcac tgagtcaact gttcgattaa aggttctgta gttttctcct gctgcatgta
     1861 gcgcgctgat cgtcagttgg ttattaatgt caactgctcg gattacgttc ttggtgattt
     1921 catttgattg ctttacaaat tcttcggtgt acggtggtgt tactatcgga acgttcccgt
     1981 ttgttgcgag aagcttttga gctgtcacca tattctggat agtattcttg actgctgcta
     2041 tatagtctag ttgtagatta gaaatcgatt gtgtgtactg tggttgagct ttagttaact
     2101 cgtctacaat tcgaatatag ttctctcttg ctgtatcgta taagttaatt acttcggttg
     2161 accttgttgt agtatttacg tcgtctttag atttattgtc tatcatttta tatctctata
     2221 tcattattag catccacctc atatatacgt tggtatttat tgggtcttaa taccatgtaa
     2281 aaccatttaa tgtctggata gtcatacaga atagtcatat ttagcaaaag atattcggct
     2341 gctagggcgt ccctacttcc gagctgagtg actgatatgt gttgaccttt attctaaggt
     2401 ccgtatacaa aggggttttt ggatcagttg cgttcttgta attcgctttg catccagagg
     2461 gtttcagata tcgaaatata tatagcaaca gttgattcgc tattagaata tgttgcaaat
     2521 ccagaagcat caactgaatt tgatgcatta tcatccttta attctactgc tagacagaat
     2581 tcactatcta cgatcaggca tgtaactttc gattgcaact tacgtgaatc gtgtctttcg
     2641 attggttgta attttgcttt accttttccg ccttttttga ttgtctgata tatgaggatt
     2701 ctagttttga tcccagaagc tgccgcctgc tctattgatt ctactagccg cccaatctaa
     2761 taacgataaa acgtattcat ggtaggaaac attaccagaa tttcttcttt tgctgattcc
     2821 tacaagctta tgagcaagat tatctatttc aataccttct tgaatagtat ctataaaatc
     2881 tctttttgta tgttcttcaa tctgcttaat tcgttgtctg gcagggagcg ccttgtgcca
     2941 tagagtgcca aatacatact gttgctgttc aataaatgat ctcacagttg tatacatact
     3001 ctgtgttatt ggttgggcct cttgtattac agcagtggcc tggtaatctc gttcgctaac
     3061 tgaaaagttg cctttaattt gatccaaatg aggtagctca tcaacaaacc ccattagttt
     3121 tttgcaataa gagagattct cagatgtaat ttcagtaatg actctatgtc gtatgcctct
     3181 tttcctaaaa tccattaatg cctgctttat tggttctgta gcgatgagca cggaatgacc
     3241 caactcatca atacatgtat cgctcttttg ttttagcaga gaaaatcgct gcagcgtaaa
     3301 attgataatg ttatctacgc cgtacaaaac ctctgtatat tcacgcttcg tgtctgaatc
     3361 gtccgatgag tgttgagagt gcgatttgga ggatgcaggt tctgccaata tggttgagtg
     3421 atagataaaa gttcatagat aatagcattt ttgggcttag ctccatatct atagcagtac
     3481 gacttttttc gtccataatg caggccaaaa gagtgggcct ggtggtggca ttatatgata
     3541 cactgatact gacctggcat aggctcctat tgccacgact gcctcaacaa atggagagca
     3601 aggattgtag gtttctctct ccatattgat ttggtaatcg tggcaaaatt tgcggcttta
     3661 tcactgaata gaattttcta cttctgtttt tgacttcctt cactaaatat ttttgccaat
     3721 ttttcgtgta attctttctt agcggaggat ttttgtatca tagcctgcca ctctttaccc
     3781 atactttcca ttaaggcatc agcaaccttg tccataacag gaccatttgc cgcttctatc
     3841 ctcttcttta gtttttcaat catcaactcc gaatacgctt caaaggatgc ttt
//
LOCUS       DAOR01000302           15901 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_45343, whole genome shotgun sequence.
ACCESSION   DAOR01000302 DAOR01000000
VERSION     DAOR01000302.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15901)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_45343"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    382..511
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    901..1058
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1854..2104
                     /estimated_length=251
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12177..12363
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4605 a         2976 c         3096 g         4498 t
ORIGIN      
        1 aaatagaggc tcggggaccc tctgttttat tattgatata cacttatgaa gcagagtctg
       61 aaagtactct aactagtgat acatcttaga tgcaacgatg tcaaatatgc taactacata
      121 ggaaaggacg tcacagtacg gggctgggta cataggattc gaaaacaaaa agaaaatacc
      181 ttcatgctaa tacgggatga cagaggcgga gtaattcaat gcgtacttgc tactgagaag
      241 gcacttactc tcactatcga gtcatgtata gaagttacgg gcaaattgca gcaagattca
      301 agagctccag aaggtgggta tgaaatcaca gcgactgacc tcaaaatata cggcgtagca
      361 aaagttgact atcccatagg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtatcaga gcgaggatct gctgctcgat
      541 aatagacacc tcgcagttag aactaggcga atgagcacaa tagcaaagat ccgggcctcg
      601 attttaaaat atgcgcgtca gtggtttatt gaaaataatt ggttggaggt aaatgctcct
      661 gttatagtaa aggatgctgt cgaaggtggt tcaacacttt tcaaggtgag atattttgat
      721 gatgatgcgt acctctcaca gagtgcgcag ctctatcttg aagcgatggt cttttctcta
      781 ggacctgttt ggagtgtttc agagtctttc agagcagaaa aatcgagaac ggttcgacat
      841 ctagctgaat ttacacatat ggaagctgag gctccatgga taattctcga tgacttatta
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga atccaagaga aattaatttc
     1081 atatatagtt cataatacgg ttcatgaaaa ttcggcagat cttggttatc ttaaaagaga
     1141 tcttagtgac ttgaaatcga tcgagccgcc tttcgataag ctatcatatg aaaaagtaat
     1201 tgaaattctt cgttctaaag attttgcaat aatcgaagat aatggaaacg agcgtaaaat
     1261 agagtggggt gacgacctga acgttgactc agaacgtgaa ttaacaagaa atcgcagcaa
     1321 gccgattttt gtagttgact ttcctatctc gttaaagcct ttttatgtca aggaagatca
     1381 tgaaaaagaa gggataggac taagtgcaga tatgcttgcg ccgcgtggtt ttggagagat
     1441 tagcagtgga ggagaaagag agaatgacat tacttctatc accaaaagaa tagagaaaga
     1501 agctctagat ccgaaggcct acgattggta cttggatcta agaaggtacg gcagtgtgcc
     1561 gcatgcagga tttggaatgg gaatagagcg tttagttagg tggatcgcga atctacaaga
     1621 tataaaagat acagtactat ttccaaggac tatgtcgcgg gtaaggccat aaagtaaacc
     1681 aagattgtcc cgaccgctat ccgtcgtctc atttgctttt agaaactaca tttatctttt
     1741 agctattagt acctagctat gacttggtta ctatttggac tttacataca ataacttgaa
     1801 aggatcttta agagatctta atatgagcaa tcccaattgt ctgccctgtt aaannnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnntttaaa ataagtagta ttatcttact aagtagcata cctagcgagt tgtctgaagt
     2161 tcgaatgtct ggcgtatgaa aaccttctgc tcgatattgt agaggcaatt cgtacatgtg
     2221 gattgaatag agactgattc cttaatgaat tggaatcgat atgtatcttc agttagcaaa
     2281 aaatatctta atccctcaat tgctgttatg atagtaggaa gtaaacaaaa atgttacagc
     2341 ataatggatg gctaactcgc tttaaacagg acaggtcaat gttatggtgt catacctata
     2401 agcttgtcat aaactgtcat attgcatcaa cccagatcgc ttgacccgct agacctgtta
     2461 gttgtccttt ggttttatgc tgaccatcta tcgacatgta tttgctctgc gcaaccaaca
     2521 cgtcttcatc agatatattt tacacgtcaa catctctacc aattctgatt ctattagcag
     2581 ctctcataat gctatcaatt actgtttagg ccttatatgg tctgtccaaa aaaacttata
     2641 caaaagtgtt aacagtcaca taagatttta ttattttatc ctaatggtcc attcaatcta
     2701 aatgtagatt ggtccactaa taaccgccta ttctcaataa cgttattttt ggcaacgtta
     2761 aactgcagat tttaatccgt attatttccg caacagaagc ggaaaagaca agaatggtat
     2821 caagttaagt agtcttctat acgttattaa ttagatggca gagaactcac tcatagacta
     2881 ttagttaggc tagtcaagca tctagaatcc tgaacttata ggatcgaagg gcttcattaa
     2941 ttcacgcaat agtgttgtag cataagaccc cttaggcagc ttaaatgata acgtcagcat
     3001 agttttgtct atgacagaga aatccctgca ccacaaagga gcttgtctaa atcctccttg
     3061 cgaactcagc tcttgcattt cttttatata aaagtctcta gccactacac cttcatcctg
     3121 cattgtatct ttcgttatta actcaaatct tcctttgcca gcctgaaaag agtagccagc
     3181 taatctaatc gctggaacta cattagactc tgaatccgat atcgagttaa actttcttat
     3241 tttgccaaat attagcggac ctcctatttc aaagcagaga tccccctctt tacctttaag
     3301 caggttttcc ccgtttctga ttgctgtact caggcagata ttgaatatat atgcttgata
     3361 cgcctgaaca aataatcttc gcatattaat tggaatgact ctcagcgcat ggattacatc
     3421 cttgccttgt aatattgcag acatcagctg acactcgata tccattcctc gaggaagtag
     3481 ttttagaagc cgagcataat tatggggatc tcgactcttt tccctaattt ccctgctcgt
     3541 tttggaatcg tattcactag tatagcataa tagtaattca acggcgtttt tgaagttacg
     3601 ccttacgatc tcccttccga cgagatgtgt tactgatcgt tcactcccga acctttgcag
     3661 tccataaaaa ttggcaatat cttttatttc acgctcgaat gtagacatgt ctaactgatt
     3721 gttgttgctg atgataatgg taaactcgtt cccgagaagt gacgcttttg ctaagggttt
     3781 tcggccaaac cctttgagag tcagaaatat ttgatcggtt cgtatctctt ttggtatatt
     3841 ttttaattgt tctgttgtag catactgctt tgtaattgcc tttgcgtctt ttaaccctat
     3901 gactttaagt ggaatgccga atcttttctt gatttgcgat atagcatgat tagaatcaat
     3961 attgcttttc tctaaaatgt aaagcgggta tctatggagt tggtcttgta cagacgacaa
     4021 atcattgaag actgccggat ctattatttc agagacttgg aactcatcgg cttgttgctt
     4081 tatcagtccg cctactccct taaagcttgt gcagtagcaa ttcatgccag ctttctgatc
     4141 gataatagga acgggaaatt ttatcaatac aactcacgcg tgccttcacc aacaagggta
     4201 gaccgctctt ccaggcacca ttttattgtg atatcactaa ccatgaccaa tcgtgtgtca
     4261 gtacatgtca atatctaaat ctatatcctc aatgagagac gatttgttaa ctgttgaatt
     4321 ttttcatcca cattaagtat gcggcaaggg tactttcata cggtaaagcg cttgaacctt
     4381 taacattgaa tccgagtagt agcattctgc cctcaagttt ccttcttaaa gagacaggaa
     4441 ggtggttttc tcttaatggc tgaaatatct caaaaaattg gtcttgttga tggcaagcta
     4501 tatgttgtgc aaaacaatct gtgagaatgg tcggatcccc tgcgattctt ttgttcattt
     4561 gagacagact aaagggggta ttcgttgtat tatcccttaa ttcgatagaa atttgtaaga
     4621 cccttttagt gtaattctcc ctcgtgaaat tagaatccat tttgttaggg gagttatgaa
     4681 acatgcgaac ggttctagtt aaatgcgcaa ctgttctctt tccgagatcg gtggcagcta
     4741 agatctcttc ctctccgtag agtccattat tttttccaaa aataaagaaa gtccccatta
     4801 gttcctcttt ttttatgaaa gggaattcta atttgcctcc aataaacagc gtaattcttt
     4861 gcgagacatc ctcaggcaaa aacatcccca tatatgaatc cgtcacgaca gccaatgtct
     4921 atagtaagga tacggcgatg atttaatcat agtcagtaac acgaaaattg aagtgttctt
     4981 tatttcgtgg cctttctgta tccgtgtgtg aaacctgcat cataaacttt tgaaaattca
     5041 tatttcttgg gggctatcac atctccgact ataataacag cagttttaat tatcttagct
     5101 tcgtgtgtct tctttacaat gtcacctagt gttcccctga ttattttttc gtcttcccaa
     5161 gttgctcgat aaacaatagc tgcaggcgtg tcttgcgtgt aagcgccact gttcataatc
     5221 tcctgtacta catcggctat caggtgaacg ctcagataaa gtgccatcgt agcgccatgt
     5281 ttagccagtt ccgaaatcct ttctcttgga ggtaccggcg ttcttgattc tgctcgtgtt
     5341 attattagcg tttgagtatt accaggtaaa gtcaattcta ggttcataac agccgctgca
     5401 ccaagtatag cagtgattcc gggaactact ttacactgaa ttccgtcttg ctctagcttg
     5461 tcaatctgtt ctctaatggc gctgaaaaga cttggatctc catcatgaaa tcgtatagct
     5521 agttttcctt tttttgtgga atctcttaat atctcataaa ttttctctct atcaataaga
     5581 gccgcatcat gaagcactgc atcttttttt ttatcttcta agatcttggg attcaagagc
     5641 gagccagaat aaactataac gtccgctcgt tctagcaatt ccttggcctt aagtgtaatg
     5701 agggatgggt cgcctgggcc agatcctaca aaataaactg tattactttc tatcatggtt
     5761 attctttttt gcgaccatta tcgaaaaata tttttcagtg ggaccttttg tccccaccat
     5821 atcacgtagt ttgttcattt tcaagatctc gccttccacg gatatgtcct gggcaatagc
     5881 tatagctgaa tcatctccaa atcctgcttc agaaagcatt tcaattaccc tgtcaaaata
     5941 tctgccgtcc ttaaggaaaa taagtgtatc gcaacaattc gcaacttttt tgattctatt
     6001 tagatcatag caagcaggaa ttattgcaac agtctcgtca ccttcagcaa gactcatctt
     6061 tgcttgtgca gcaaatgcaa acatcgatgt aacaccaggc acgatttcaa tttcgatatc
     6121 ttcatgattg caaactaatt ctctgtgaat gtaaatccat gtactataca acgctgggtc
     6181 acctactgtt aagtatacca cttttttgtc tgatctcact gcttcggcta tctctagagc
     6241 atttctcttc caatagtccc tcaacgattc tctctctttg gtcattggaa atacgaggct
     6301 aacggttcta gctgattttt tcaaatgcct ttctactacc gaaagggcga tactcggctt
     6361 gccgtctctg gcggtgggtg caaaaattac atctgctgct ttaactatat ttacggcctt
     6421 gacagtaagc agctctggat cgccgggtcc gcaacctacg cagaatagct tcatgcatcc
     6481 aatctctcta cccggattat ttatattcac tttctcttat tttcaattaa tagcaagatg
     6541 tcagcaaaat cttcctcatt ttggctttga tattatacta atatagtctg attcatggct
     6601 ttgtggcaga aattatcgtt atcggatttc gagcaaccat cattgtacca cttgttatct
     6661 tcttagcctt ggcaattgtg gcctgtgtaa tatcgacttc gcaaagctga gcttcattaa
     6721 ttgtactgat tgcatcatac aatgtttcga ctagaatagt atcgattact attctacctc
     6781 ccttctttaa tcggtctatg cccaaacgaa taactcgcgc cgtgtcccct gagctcccgc
     6841 cgattataac agcgtcagcc tggggtatgg atggcaaaat atcctgagca gaggagaaga
     6901 ttacctcggc tttcacccca aacttaaaga gattcctttt agttagttct atcgcagttt
     6961 tgtcgaaatc aatggcatat actgtgcctc ctcgggcttg caaacaaagc tctacggtaa
     7021 tgcttccgct cccgcatccc acatcaatcg cgcaggaatc ttctctcaaa cgtaatttac
     7081 taattacgag cgctcgtatc tcctcctttg taattggaac ttcctcagac ctttcaaata
     7141 attcatcagg aatcccgcca gtacggtaag tccaaagcat tggattcatc ttattttgga
     7201 acaccgtcta ttttaacagt ggcatgttct gatgcaatcc agtataaact gcaatcataa
     7261 acataattag catgaaaata aacggaaata ttccgttggt agccaacttg cgtctctgcg
     7321 gctttgcgat attcttagcc atacgctttg cgacttgata cgatataatg tagaatagta
     7381 gccctatcaa tatcgccgtc ccaatagcga cgccttgacc agttcccggt gcaatagcaa
     7441 aacctgtaaa tataccaccg atcaatccaa gaatggctct catgtaaaaa actctgtcaa
     7501 gaggatccaa ttatattact tttgtcatta atttatacta ggtttaattc cttgcggttg
     7561 tccgtcagca tgtgcgaaac gtttgtacgc agtgtatttt ggttaatatt tgtggaagat
     7621 ctcactatca ttggttatgg agctatcagg catcgaactg agatatctca tcagcgagat
     7681 taattcaaag atcacttcag agtattatgt aagtaatatc gttgcggtga caagagattc
     7741 ttttcttttc cggatgcatc attctacaga gcccgatatt acgttaatgt tatctgttcg
     7801 aggcatatgg cttacaaggt tcaagttcaa acaggtagag gagagtgacc ttatcgggat
     7861 cattagacct gaggtcgaga gggccaagat cgaagctatc cagcaatctg gtagtgagcg
     7921 cattatcaca ataaggttta agcattttga cggtaaatca agggtaattg tggccgaact
     7981 tttcggtgtt ggaaacttta tattgtgtga tgaaaagatg cagattctcg ctattctaaa
     8041 tcctattcaa gttagacaca gagttctgaa gaagggacac caatattttc ctcccccaac
     8101 tcgcggaact gatgtattgg atatcacact cgaacagtta caaattatgc gcaataggac
     8161 agaagcaaaa gacctggacg ttttgcggtg gttaggcaga aatatttcaa ttccaaaaaa
     8221 atttgtggaa gaaatagtta ggagggctgg tgtttctgct ccaaatattg gacaactttc
     8281 agatgatgac atgtctagaa tttatacagt tacaaaggat cttgttgcag acatcagtac
     8341 aggaagtcac gaagcacttg taattctaga tgacgatgga aaagcacgag atgcagttgc
     8401 tattccaact acgaacattc cttccttgaa gccgataccg acgacgagga tgagaacagc
     8461 ccactcctac atggaagcaa tagatgaggt tctagccaac gagataacgg aaagacatag
     8521 caattcgaaa actactgata ttgataaaag aatagcaatt ctagagcatg acctagcaga
     8581 acagaataaa gcaaaggagg aggttatttc gaaggccgcc gtcataagga agattgctaa
     8641 tgacttgatg gcaatctcct tcgagggaac taattatact ttcgatgacg attcaatgaa
     8701 agaattcctt ggtgctagct ctgcgcttat cgttacagat aaggggagaa aataccttga
     8761 agtactcggt gaacgtgttc cgacagcagg ttcgagcttg gcaaaaatat catctctttt
     8821 gtttggaagg gcaaaagagt tggaaagagg ctgtatatct attgaggacg cagaggcaaa
     8881 actgcacact caactcggta atttgagaaa tcagacaacc gcaatccagg acaagatcgt
     8941 tattaaagag caaattgcta aagaatggta tgagcgttac agatggttta ttacgagcga
     9001 aggattgctt gcaattggcg gaagggacgc atcatcaaac tcggccatta ttcgaaagca
     9061 tttgacagaa gaagacatgg tatttcatgc tgaggtacat ggttcaccat tctttatcct
     9121 taaggacgca aagcaagtta gcgaaatagg tgctagtcgc atggaggtag cacaggcaac
     9181 tgtctcattc agccgagctt ggaaagacgg attgttcagc gccgatgcct attgggttga
     9241 acctagtcaa ataaagagag gtgctccgac cggacaattt cttccgagag gttcgttcgt
     9301 aattgaaggt aagcgtaatt acataaaggg tatcgaaata aagctagcag tcggacttgt
     9361 acggtcagat tcgcactatg ttcttgcttg cggacctacc aatgctataa agaaacgatc
     9421 ggtagtttac tcgcaattat tgcctggggg gatggatccg aacagcgtag caaaaaaaat
     9481 taagacagat ctagtcaagg cagcctatac aaacacaaat gccaaagatg attcgaatgc
     9541 tcagctagca gatttcacta aatcaattga tattggtgat tttattaggg caattccatc
     9601 aggacattca aaaatttttc tgactgaaaa aggggaaggg gaaaagagct ctctggcaaa
     9661 gcagagaatc gatgagcaaa gtaattaaag ctagaaatga attcactgtc gtggtaaaga
     9721 aagagctagc aagacaacat agagacgcta atatgccgac aagagtactt gttagagata
     9781 taatgaacag tccggtggtt tcagccgctc ttacagatac agttaacgat gttgctttaa
     9841 agatgaaaga tcaaaaggtt ggtagcatta taataatgga aggagaaaat ccagtcggaa
     9901 ttgttagcga ctgggatatc gtttcaaatg ctgtggtcaa ggacgtcata ccaagtacca
     9961 taagagtttc agatatcatg caaaaactcc actccataga aggggaggag ggcgtcaccg
    10021 aagctgctcg agtgctacga caacatacta tcaaacggtt gggtgtagtt tataagaacc
    10081 gactggttgg cattatttca gcgtcagacg taattgcggt taccccagag ctcgtcgatg
    10141 taatttctga gaaagcagca attgttagag gggagactgg ccgttctgct ggttctgttt
    10201 ctggatattg cgacgaatgc ggggaatggt ctgatctttt gcaatttgct gatggaacct
    10261 ttacttgcga agaatgtagg ggcgaggaag tcattagtta gatcaaaacc ggccttccta
    10321 gtcgatgcga tgctcggaag tgtcgctcga aaattacgtt tttttggttt ggacacgttg
    10381 tatatcgcag acagcccaga cgatgagata atagcgatcg gaatcaagca gaatagagtg
    10441 attttaactt gcgacaagga tatgtatagg agaattatga aagctggagc acaggggatc
    10501 ttgctcaacg gtccgaatga cctggaaaat attgcacaaa catttttcag tttcgggatg
    10561 atgctagggc cttatataga cgtcagttct cgatgttgta tgtgtaacgg attgttgtct
    10621 gagatatcgc atgcggatgt taaaggaaaa atctctttca acattatcaa ccggcataca
    10681 caatttttcg aatgtaaaaa atgtaataaa ctcttttggg aaggtagtca taatttaagt
    10741 cttagagctc tgtcaaaaag gatagacagc cgtatcaagg agcgactaga aaaaaataat
    10801 tcttatgcca ctgatagcat tagtcagtcg atcgtaaaaa agcccctaag ccgtcctgtt
    10861 aattaagtac aaagacggtt gaaaacaatc aattgatgtc ttgttatctg ctagtttcga
    10921 tcgtacagaa cacgctggtg aaagaagttg tagtttctct taatctatca tacttttgtg
    10981 ttcattgttg cataccttca tcagagtatt ctgagtgtgg gtcttacgat aatactaaga
    11041 ctttcctcag atatacacca aagcgaagga gttacttctt ggcctttttg ttcacagccc
    11101 atcgttcata aatctttatc aattcatcta catcttcgcc gctttccgaa tatgtaataa
    11161 tcacggcaaa tctacctttg ccatcatgtc cccttgcaaa ataaccccag aacgtgtccg
    11221 gcagtctcgc aaagttaact ttgtttttgc tgacgccaat cagattactc gatatcgtat
    11281 caataacccg gacggcatcg tcagcttcta acattagaga ctcgatcatc agtactactt
    11341 ataaaatctt aggacctgtt ataatatcaa atataggtat aaatctgaag ttagtaaggt
    11401 ttagaggagt ccgataaacc aaagcaagag cggtaggtaa agcggtatga gtgacgttgg
    11461 gtgtttttgg atcaggagac taattcattt attgtaagtg tcctgccaca gtacctacat
    11521 ttcattatta tcttgtgttt gtctatgata tcgatagtag ctctgatatc ctcttcacta
    11581 tttgtgacgc aatttaaatt cggacagcta aaaattccca taatggaatc ggggagctgt
    11641 atctttcgct tttctaccaa tttgtattct cttattatat tgatggttgc atgtggtgca
    11701 attaatgcta gcttattagt ctcattcttc tccaaaaatc tattttctac cttgataata
    11761 tctttcttct cgtgtttgct actcggaacg tttaaagcga cagtgatgac gttgccttct
    11821 ttacctgtaa tattgagcgt gcgcaaaacg agcatagcac gaccagactc aatgtgatcg
    11881 atcaccgttc catccctaat tcgtcgaaca agaagctgac tactatcgga catagcctat
    11941 gttcaccagt attactatct ctaggtagta ataagcataa tgaaatttgt tctcttaata
    12001 ggtccacttg ataatgtaaa gaaaaggcac aaccaacgtc gaccatgagg atgctgacga
    12061 gctagacgaa aacaatgccc caatgatgtg agaattaact gtacggggta tatcgattgt
    12121 aatggacgta tcatgatgac aaccacgttc gactcctcac tcactattta tgagtgnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnncctcact cactatttat gagtgattgc atattatgaa acatcggctt tagttccgag
    12421 gccggtccat gtatatctat tcttgtggtc ccctggatgg aaatctttga tattttaaac
    12481 attagccctt tttctgaata ttccagtccg tgaagtcgat cgagtctctc acttacaata
    12541 tcatctgact taagaatatg gcccctctgt gcgaataaaa ttatccttct gtccgttatc
    12601 aagacgtggt attttttatc cccataccga acagaagatt tgctatgaaa ttttacgttc
    12661 tccaaaggga gtaaaaaatc ctgaagcggc atgaaaggta cactatgaat atttgacaaa
    12721 atatatatcg aaaagagttg aagcaattta tcaaaattgc caataaattt aatcttaaac
    12781 agcaagcaaa atgcttttaa aaatcattat attgggtaga attttcgttc ctgaaaatgc
    12841 taatgaagca cgctgtccca aagattaggt aaactatttt gccatagttt actcgaatgt
    12901 tttagcatct accgtaagct tttattatga tttgcaattt aataacatct tgactcagca
    12961 agaacatatt ctggggggca aaaaaagaag gtttgaggat ttgagagagc taccagaaaa
    13021 tcgtcctgct atgttgctat cggcgatcta tttaggtgaa gagcaaaaag tttacttgaa
    13081 gttttatgat cttcatgagc attctatctg cttttggcgt gacagaacaa atcacaaacc
    13141 gtattgctat acaaaaatgc aatatttcga tacagtcaaa aaaattatcg agacagaacc
    13201 aaaattcacc ctggaacaga caaagaaaaa ggagttgatt accgataagg agattaatat
    13261 ccttaaaata attgctcctg atcctctttc aattggcggt actaataata gtattagaga
    13321 aaaagtcacc tcatgggaag ctgatataaa atatcatgaa aattatctct atgatacagg
    13381 gttaattcca ggtgcatatt attttagaaa aggagaagaa attagcaatt ttgaacacca
    13441 aatttcagac aaggttcaac ttgaactcaa gaacctattg tgggataaaa ttaaagagga
    13501 agcaggagga atcgggaatc aacaatatca acagtacatt acgagctggg ccaacctcct
    13561 caaccaacct attcctgact taagaagact ttctgttgat atcgaagttg aatcagaaga
    13621 gggtagaatg cctacgcccc gtgatcatga tagaactgtt attgctgtgg gattcgcagc
    13681 aaacaatggc ttccgaaagg tactcgtgtt agaccaaaag aaaactcagt atagtatccc
    13741 gttcattccg gaagcagaga tctgcaacac agaaaaagag atgttacaaa aagcatttaa
    13801 aattatcgat tcatacccga ttatacttac ctacaatggt gacgattttg atctgcccta
    13861 tctttatgcc agatcacaag atccgagaat tgatccaatt acaaaactgg tcatcactaa
    13921 agatgaagta ccgatcatag tgaagaggga aacatttgtg aaacgaggga ctcgagcaga
    13981 accagttcaa acgagacatg gcttgcatat cgatttgttc agaacttttc agaacagatc
    14041 cgtacaacta tatgctttta gtcacaaata ctcagaatac acattaaatg cgatttgtga
    14101 agctctcctt aatgacagca aaatcgaatt tgaaggtgat ataagtgatc ttccaatcca
    14161 gaaattagcg gaatattgcc tcaaagacgc tgatctaacc cttcgcttga ccactttcaa
    14221 cgacaacttg ttgatgaaac ttttagtggt tatctgtaga attggcaggt tacctctaga
    14281 tgatatttcc cgtttcggcg ttagccaatg gataagagga atattatatt atgaacaccg
    14341 caggcgaggt gccttgatcc ctcgacgttc tgagttacaa gaaaaaggaa ctgcttcaac
    14401 tacagcaata attaaagaga aaaaataccg aggcggcttg gtagtcgagc caacactcgg
    14461 gatccatttt aatgtagtag tagttgactt tgcaagtcta tatcccagca ttattaaagt
    14521 tcacaatctt tcttatgaaa ccgtcaactg tccccatata tcatgcaaaa gcgacccaaa
    14581 acagcatatc ccggaaacga cacactggat ctgcaaggaa aagagaggaa taacttcgct
    14641 tttaataggc tccttgcgag atttgagagt gaattattat aaacatctat ctagggacaa
    14701 aacactgagt catgaagaac gtcaacttta cggagtagtt agccaggcaa ttaaagtaat
    14761 tcttaatgct gcctatggag tgatgggggc ggagatattt ccgctgtact gcttgcctgt
    14821 cgctgacgca accgctgcta ttggaagaag taccaccgct aggacaattg agaagtgcaa
    14881 gcaaataggt atagaggtga tttatggaga tactgactca ctttttttga aaaatccttc
    14941 aaataagcgg gtggacgaaa ttgctgcctg ggctagaagc gtccttggcg tagacctaga
    15001 gatagacaag caatatcgct atgtggtgtt tagcgatctg aagaaaaatt atctgggtgt
    15061 cctggccgac ggtaccgtag atgtcaaagg cctaactggg aagaaatccc atactcctcc
    15121 gtttatacga agggcatttt atagtatctt ggatattttg agcaaagtaa attccgaaaa
    15181 cgacttcgaa tctgcaaaac aaaaaatcaa aacaatcatt caacaaaacg cgcgcaattt
    15241 agagcagaga aaaataccac tagaagaact tagtttcaat gtaatgataa ataaatctcc
    15301 tgataaatat ggcaaggcca cgggatcaag ttgggccatg tcattagacg gcaaggatgc
    15361 gaagacagga acctacaaag gtctgcctca acacatcaag gcagctaagt tgctagtaga
    15421 tataggcagg gacctgaaag caggagatat tgtatcgtat gtaaaaacaa taaccggtga
    15481 cggggttaaa cctgtggaac ttgcaagacc cgaagaggta gatacagaaa aatacgtaga
    15541 cgccatggaa gcaacgtttg atcagctact ttcgtcacta aactttaatt tcaaatcact
    15601 cttaggcaag ccacgacagt cgagcctaga tgatctattt tggggtaatt aatttttctc
    15661 gctgactgct aggagaaaaa gctaaaacta atagagcttc ctttccttat gcggagtggc
    15721 gcatatacag gtttccactc gattgttaat agagtataat agaccgatat gtgcttatgt
    15781 gcacactggt ggccgttaga tattggtcgg aaatttgtgc tggcgattgg acgatgcata
    15841 ctctagaatc tgagtctgat ttaattctat agattttatc tgctatgttg cataactgag
    15901 t
//
LOCUS       DAOR01000303            4487 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_25181, whole genome shotgun sequence.
ACCESSION   DAOR01000303 DAOR01000000
VERSION     DAOR01000303.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_25181"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    908..1118
                     /estimated_length=211
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1260 a          889 c          742 g         1385 t
ORIGIN      
        1 caacttctcg ccccataatc tggcgcttca agtgtgcatc agcattatct tcacctgtct
       61 cattgtgttt atattgttca gtaggagcat gtggtgctaa tttttcaagc catatatcaa
      121 aatcttcatg caaaccactc tcattatcgt ttataaaaac gctagctgta atatgcatcg
      181 catttaccaa acacagacct tccttaattt tgcttccttc aacagccctt ctaacatctg
      241 gagttatgtt gatgaatgca cgtctggtgt tgatattgaa tgtaaggtat tctgttttag
      301 acttcatctt tgaacctcat atttgagcaa gttatattct taattgttaa atgttagtaa
      361 ggatagcgga caaagacgcc aatacttgta gataggagtt tgccgatcac aataatccaa
      421 aattagcttc aagacgaatg ataagtttaa ccaaatatca gactactact taacaggatt
      481 aacttcgatc agcatttcgt tttatccatt aacatactta tatcagaaat tttcggtttt
      541 ttctggcaaa gcctaccgta atatccaatc tctaaggtca acgtaacaaa aagaatgtat
      601 gttctaatac tcaaaaagca tatgaaatac gaaatggcta ccaatctaga ggatctacag
      661 gatagtagtc ctcaactcac acaatccatc tattaccgaa gttgcaaaat tcgtagtgca
      721 agtaattttg tgagtcattt tcgaatgaat aacacgtacg acagattttt acatttctag
      781 tcttgaacta atttaagaat ggtatttccg tggtcaacat ctacttaagt tgttaccaag
      841 aggagaaagg tcggtaactg aagggaatga tggcatcatt ggattagatc ccattaggtt
      901 atctttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cattaggtta tctttcactt
     1141 aaaactagaa ttatgataaa ttagtactac tgacatggta acgatgctgg ttccatttta
     1201 agcgctttta tggacggaaa atcatcaata atcttcgtga gttccctctt tgcacatttt
     1261 agacataatt caataaccga tatatcatct gagttgaagt cttcaataga caatgtgtaa
     1321 taagcatcaa catcacaatg cgtacatcgt tgtactcgtt ttactaatat gttcataaag
     1381 gtatactcca gaatcataga aaattttgac gtgatggaca tgatggatcg tatcggtgac
     1441 cattacatcc gtgctaacac tactactgcc gtgaaattgt tttgcgtttg atgttactaa
     1501 ttatggaact ctcatttgga atcccttgtt ctctgcatgc tagttaggga gcagcggcta
     1561 atgctgctga tgtctcccca ccccgtccta atcttctttc acgggctttt ctgtagatgt
     1621 atgcagtttt ctcaaatatt gcatctggta tcttatcggt aatgatcgtc tgatcttttt
     1681 gcatgtttag catgcaactg aattttggat tatagttacc atgttaatgc taagctatgt
     1741 taatgatact cattcaaata tatgaatgac tatactgctc cgcctttgtc ttgagctgtt
     1801 ctgtctacaa tataactaat ttttggtgtc atctagtcac acctacaaga ttgtaacttg
     1861 cttatagtac acctctcaag cgaaattgca ttgaaaactc cttttttatg ttcaccgata
     1921 tagcccgttt atattaggac gacaaccaat aacctttaca aatgagttac ggtagaggca
     1981 ggagttatgg tggcggcggt ggtagaggct tcgacagagg ttctactacc ccaaaaccag
     2041 tcgaagtagg taaggaatac gaagtagaca ttatagaaat tagtagaaaa ggagatggaa
     2101 ttgcaagagt gcagggtttt gtagtttttg tagagaatgc aaaagttggg aacaagcata
     2161 aggtaaaagt caccgatgta gcagatagat tcgcaaaagc ggttatagtc acttaaattt
     2221 cgctatgcga gcatttaggt ttcgtataaa taaaagtagt attaaaacca tcatttcaat
     2281 ctaacctgtc caccgtgttt catataatcg atgtatgaaa aaccacaggc tgatgaagag
     2341 tactttagac taattcagca tactggaagt aatgaataac gaatccactc cgctacgata
     2401 accagataaa aaacgtgcag gcagaatata agtaccaatg atcaactaaa taataataat
     2461 aattaatact ataccgaatt aatttctttc acccatctat tatgggtaaa atccagcgga
     2521 ggcaagtgat cgatacatcc taatagtacg cagcattaaa gaaaattgta aaatattctt
     2581 tggaaaataa atgcgttaaa tgtaatctac aaattcatcc cctcttcata ggatatcata
     2641 aatgtggtta tggaaactgc cctgtttgca aaagtgcaat cacaaattca gaacactgga
     2701 gtcatattag atcacatcca ggacacgaga atgatgcccc ggcgccacga aggagtacca
     2761 cctttgataa cacagggaat agaggcaacc gtcaggaccc taattgacta aaatgactca
     2821 ggccatgata tatgtatcac cgagcttata ctttttaatg cactaagatc tgccaataat
     2881 gctggacaga gaagaatagg tggacacgga tcttgttagt gtaaatatat agccagcgca
     2941 atccgtttcg attttcatcg taagactgtc ttatcaggga tagcacatat tctatgggta
     3001 tgaataacat cagttttcag cttgcacatt gttgtcatgt tcttatgcct aatctactcc
     3061 atatccattc caccgccgta gccgccgccc atactaccca ttcctccacc accgccaggt
     3121 gctccaggag gcattttgga cttgcttccg gtgattacat cgtctattct caataacata
     3181 gaggctgctt cggttgcaga ttttatgatt tgttccttaa ctacaagagg ttctagcaca
     3241 tcttgtttaa acatatcagt aacttttgta tttcggacat ccactccagt ccatttggcc
     3301 cccttgtttt gttttgttcg tagttcagtc atggtatcaa ttgaatccat acctgcattt
     3361 tctgcaagag ctagcggaat tgaatcaagt gcatctgcaa acttttgaac tgccagctgc
     3421 gctcttcctt cgagattgct tgaccatcct ctcaattcgt ttgcgatgta cgcttctgta
     3481 gcaccgcccc ctgccactat ttcaggtctt tccagaacat cctttattac catcaaagca
     3541 tcatgtattg atctttcatc ttcatcaact acactctgtg agccacccct cacaagaatc
     3601 gtcactgctt tgggattctt gcacccttct ataaagaccc atttgtcatt ttcgatccta
     3661 cgttcttcga ccaaatcagc agaacccaga tccctagcat ctaaactatc tagattactt
     3721 actactttgg cacctgttgc tttagctagt ttgaacatat cactttcttt tacacgcctt
     3781 actgcgtgaa tagctgaact tgagagataa tgttgtacta tatcatcaat acccttttga
     3841 catactacga cattggcacc tgcttccata atcttgtcga ccatagattt gagcatcttg
     3901 ttctcttcat caagaaaccc ttgcatttgc tgagggtcat taatacggat ctcagcattc
     3961 atttcggtct tttcgatttc caatgcagag ttaatcaatg caattttggc atttttaatt
     4021 agcttcggca tccctgaatg aacaacttcc ttgtcaagta tcatgccttt gatcaatttc
     4081 gtatcatgta tagaggcacc tgctttcttc tcgaccttta tattgtcaag gtctactctt
     4141 agactaacac cattaacttc atttttttct gccacctgtt ttgttgcatt tacagcgatt
     4201 tgacttaaca aagggctatc ttctgatact agcttggaat acatgctagt catagcattc
     4261 ttcatcaaga gttctttgtc ttctacatcc actttaattg aaattttctg taatagtttc
     4321 agtgcttgta gagaagcagc atcataacca tcaacaatta ctgatggatg gacattcttg
     4381 ttaatcaaat cttcagcctt tcctagttgt tgtacttaca cacataggta acaaattcac
     4441 ttacagactt aggttacact tctgctagga agtgtatatc ctataga
//
LOCUS       DAOR01000304            7226 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_35136, whole genome shotgun sequence.
ACCESSION   DAOR01000304 DAOR01000000
VERSION     DAOR01000304.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_35136"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2150..2382
                     /estimated_length=233
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6373..6597
                     /estimated_length=225
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2247 a         1249 c         1318 g         1954 t
ORIGIN      
        1 actagattag cctgagattt agaataatat ttatgacctt ctagaatgcc atctattcta
       61 tattttagtg atgatgtcaa ggatattaat tctggtagtg ttacgtctgt tctaccttta
      121 agaacgcttt gcattaattg tttgtaggta atatgatagt agttgccgtt accatgaagt
      181 ttgaatattg tgttctcaga ttcgtctata gagtctagaa atgctttaac attgagaact
      241 ccattattaa taatggtcat cccaatcccc caggcttagc gaactgaagg cagctgcaat
      301 ttttaacaag gcaatagtcc ttccccagtt tgtgatcaga ttgtttgtcg ccacaagtgt
      361 tacactccag ccgaccttca tattcctctg tttgtgttgt catgttgcaa caaccatcat
      421 ttccttctgc cacttggctt tatacactga attacgtgga cgacttctca tcctagtacc
      481 gcagcaagga caaaacatac cgtcacattt caggtataca caacatacct gacatctttt
      541 atgcccatgt agatagttag attgatgaaa tcgtcgtgta ctgactttgt gacgggagca
      601 tatgccaagg catatggctc ctaacttatt tcaactcctt tatcgttagt tgtgctggca
      661 ttttctatct ctttgatata gtcacagaat ccacaacaac cacaatactt acctgtatct
      721 ttgcagtcac aataacataa ctctaaacaa catttgcaag tcatttcgtg gatttacctg
      781 ctgctttact tttctttttc ttcgccgctt tctttgtcat ttcttatgat tcctctcctt
      841 atgttcctta tagaaatgaa tcaacctctg aacgatatca tcatacgttt ctatatgtat
      901 atcacccaat ttatccaatt ctgccctagt ctttaccttc aattgaactg ttgtgatgcc
      961 ttccctttga accaatattt ctcctgattt catgagctat ctattaatgt agcttcgttg
     1021 tgctatataa ctatatcggc acagtactgt agtgctacag catatttata agtctcccac
     1081 gtatatacaa agacgataag aaaattgtca caaacaaaat caggcgtaca acgccaacta
     1141 ctagacaaag aggaaattct aaaactagag gaatacagga aactatgtca acccgcagag
     1201 tccaggtttg tcaaactgat agacaaacaa agcatcaatg ctgtcttcat gttgaagcac
     1261 aaagatacag gacatagggt actagaagga atcgacaaga taactggtca accatacaaa
     1321 agtgaaagga tgaactttgc tattcttttg aatgggaaag aagagaaggt ctttgctaca
     1381 agtaagacag atggaagatt gatttttgat gcgatagaga agcatggtac tgcattaaca
     1441 gtcacaagat acggtactgg aacggcaact aggcatattc ctacaccttg gataaaggag
     1501 caacaagaag acgatggtgc ttatgaagct tacaaagctg agcaggctgc tgggggacac
     1561 taatttttat gcttgaatag ggaatggtag aaatgaccga accatcacca gaaggaagac
     1621 ttagcgacaa agcgatacag tatattgaat caaatatgtc taatgagtgc gtatgctctc
     1681 attttagaga agatcacaat gaaagaaagt gggcttgtaa atatcctgtc acggtctgtc
     1741 agtgtgacga gtttaaacca ataaacggat cacgactatg gaaagaagca tctgaacgtg
     1801 atgagtctgg acagtggtga cacagaaata acactagata gatcacgact aaatctcatt
     1861 aaacattata ggacttatta cagtaacagc aagaatgagg aaccattaac aacatatcaa
     1921 gaagaatcta gaaagcagtt cggagaggaa tatgaaagaa atatcctgat gcgacttcta
     1981 attgacaatt ggactgggaa agactttgat gaacaagcaa aaataactca tggaatgtca
     2041 catggttcag gtcgacataa gaaattgttg gatgaaagta gggatcatat gttcaaagta
     2101 tcaaagcaga tgacagattc agaaattaat aaaactctaa acgtattaan nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagttttat ggctgaagga
     2401 ttcggtgata tattatatca ggaaggcgag cttagaaaga agaagggaaa aatggattgt
     2461 caatagtttt atggttgaaa ggatgggata ataaatgacc tgcgaaatta aactacgaaa
     2521 gaagcactgt gatcaagaag gcgtcatagc tgttgcagag tcattttatt ggaaggacaa
     2581 aggagtagaa tgaagacttt aatcagtatc gagacattcg ataatattcc acctcaaaaa
     2641 acagcttaca gtcagtcata taagataaaa gtttgtaccc tatgcagtaa tgaggccact
     2701 aagatagttt tgtacgatat tgacggtatt attcttcagc agcgatactg cgacagatgc
     2761 attaagagga taaagaaata gaataatgaa agttaaaagt ttatcatgtc actcatgtaa
     2821 taatcatgta gcttggcatg acgctaatga tgtaggagtg cctgaagtat attgtaattt
     2881 ctgttttaag catgaaaaaa tggaggtgga ggaataaatc gctagacatg gtataattgt
     2941 tagtagactg aatgttattc ctagtcatca taagaaaaac agactgttca atcatgcttc
     3001 tagaaacggt tacgaatgcg agtcatgtgg ctccgttaat ccgtttctta cagtagacca
     3061 tagattatgg aaggctgatg gtggtacgaa tgatcataaa gctaaatatc actaagtggt
     3121 aatactactc gattaatgtc aggacgtcga gtcagattca gaggatataa acagagcaaa
     3181 cataatcgtt attcgaaaag aagattagag aagatattgc gtggataggt tttagttact
     3241 tgcgttacat acacatcaca tattcgtaaa gtttcaaaag aatatccttc ttagtgtaaa
     3301 tcctgtctcg acactcatgg accaacttga tccattttca gccgaaatgg ccagatagat
     3361 ataatacatt agtatcatcg acatgatctg atgctttttg tcttttttta attatctata
     3421 tctatataat ggacagccac accttttacc gtacttctca ccaccttcat aatgactctt
     3481 accaaaacct gcaaactata ctgaattgta agataaaggt ctgaattgaa agatgatggt
     3541 ggtgtactga tgatcatgat gacgaagggg tattgtgtta cttctgttac aggcgaccac
     3601 cgataaagtt gtactgaata aataccaaac aaactttcaa aatatgccaa ataggaaaag
     3661 actgcattcc acattttaga ttaaattaaa aaatgtatac tataagcatt tgaacaaaga
     3721 aaaatggaaa caaaaaaaaa ttattgtttg tggaggaact ggaaccgcga taggcatgat
     3781 tatcatccct gcatccgccc tagaccttca gaatgagctt gtatggtgaa ctatgacctg
     3841 tcataagctc aatacagagc actttaaaga ccactcgtgc tatattttct gactattttg
     3901 gtgtagagtc gtctccatac caaacgattg attgtaacaa ttactgcaga cattattccg
     3961 actaatatga gcagcagcac ggtatttccg agatcgtagg cagcatggtc tatgagtgaa
     4021 cccataccaa gtactgaata tgtcttatta ctaaatacta gaaattctgc tactatcaaa
     4081 gcattccatc ctcctcccca gcctgtaatg ctaccagtaa taaatgatgg atatatagat
     4141 ggaaataaaa cgcgtttgag gtactgacgt ttggtggcat tgaaagcttt tgccgcatcg
     4201 tctatatcag aagatatcga acgtacgccc ccaattagat tgaaaagtat ataccattgc
     4261 atccctgtga gaattagaaa gatggaagga atttcaagcc cacctggtat caggggtata
     4321 agggcggcta caaagggaaa gaatgctgta gctggaatcg aagcggcagt ttgaaacaca
     4381 ggcattattc tactaaaatt tgggctgtga gctatcttga tggccacagg aatagtccaa
     4441 gcgagtgtta tgagatacgc tgaggccaat ctaaggtatg acaatagtaa cgccaaaggg
     4501 atttgagaaa tcaacttctt actagcggca ttaccgtata gtgcctggta caaattggga
     4561 agagcggaaa aagactttga gattactcca gcttctagaa caataaacat gatcagaact
     4621 gctgtaacaa ctaggacact cgatattcct attagtttga gatttctcct attcttgact
     4681 aacgatagcc agttcagaat agggaatgta tacaactcga taacggatat gaattttgat
     4741 aaaatttcta tcactctatt agattctaca tgtatcaatg gcctaggatg cagaattaag
     4801 aaatctgaga agtgaagcga tggaaacgga gctcttctaa ggaagatcct agctaggttc
     4861 tgattgcgca gatacaaacc aaactgctgt atgttgtgcc tatgagcagt accagtaaca
     4921 acatctgatg aggaagaaga ggaatattca tatttaaaac ggtttgcata tgattctagt
     4981 ggtcgccaaa agaagaagtc agttagcata ataatagaga ctaacaaaac catcgccaac
     5041 agggattgca gatattctcc agaactgaca gaagtactca gtaagcttcc taagcctgga
     5101 agagtatatg tcttggaacc caaagatatt atctcagccg ctgtgaggaa ataccatcca
     5161 cctgcccatg acattatact gttgtaaatt aatttaggta tactagcggg tagaaagact
     5221 cttctaaatt gcagtaatga gccagctctg tatgactgtg cagtttccaa taattctcct
     5281 gggatagaaa gcactgattc ataaactgca aatatcatat tccataccat actggtaaat
     5341 attagaaata ttgctgctat ctctattcct attggactac cattaaaaag agtgataaaa
     5401 aaaataatag ctgcaggaaa gaaaccaaga atcggaactg attgtaaaat atctatgatg
     5461 ggtatgatta tcttagatgc taatggcttc ctagctgctg taactccaat tactaatgct
     5521 gcaagaaaag aaatagccaa tgctgcaaac attctagaga ctgaagagac tgaatcaata
     5581 actaggtcag taacatttat tttcaaaccc tgattagagc tattgtcata attggtgata
     5641 ttaagaaagg gtgctttatt cacaaaaaga gctaaaaata acacaatgat tattgtaata
     5701 gatataccga ctgcaagtaa aataaatatt cgcctgttca cttgtaacac gctctacatc
     5761 gttctatttt ttgctatctg ttagagctat aaaaattaca ttattctaag cacgacatcc
     5821 ttgcaagaca accacagaaa aagaaggctt cacacacgct taactaacat aatgcacaag
     5881 gtaatctaaa caagtaccat gtagtggttt cgaactgagc ctttatctct tccattgcaa
     5941 ggtcttcaaa cgctccgtta agtccatacc cagcattgtt taccaacacg tcgattttgc
     6001 cgacatcaga tattatcgaa tgtaccgcat cgtttattga tctgtcttcg gtaacgtcca
     6061 gctgcgcaac acgaagaggt agcccctctt tggctgcagc tgattttatt acttcacctt
     6121 ttcctagatt cctcattgtg gcgtaggtaa agaagccgct tcttgccaat gttaaggacg
     6181 tttcaaatcc tatcccgctt gagctgcctg taacaacagc aaccttctga ttcgatgaag
     6241 atattgaaga gggcatgtct tattctctgt agactacgat tacattacac tacttaagta
     6301 ggatagtaac ctctggttag ttagtaacca agatctaacc aacttactaa gaaatgacta
     6361 ccatatttaa ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
     6601 gaagcgtatt cggagttgat ggttgaaaaa ctaaagaaga ggatagaagc ggcaaatggt
     6661 cctgttatgg acaaagttgc tgatgcattg acagaaagta ttggtaaaga gtggcaggat
     6721 atgattcaaa aatcctccgc taagaaagaa ttacacgaaa aattggcaaa aatatttagt
     6781 gaaggaaatc aaaaacagaa gtagaaaatc ctattcagtg ataaagccgt aaattttgcc
     6841 aggattgcca aatcaaaatg gagagagtaa cctacaatcc ttgctctccg tttattgagg
     6901 taatcgtggc attaggggta tgttctcggt cactatgact atatcatata atgcctctag
     6961 caccatggcc attctttttt ttcaaacagg atgccaagtc tatactacac tgggttagga
     7021 gggctagtgc aattcggtgt gccctttggt attcttatcg gggatagaat acaccagtct
     7081 agtcctatct tgttcttttt ctcttctaag ttgtaattgc ctcagccttt ccttatccct
     7141 tttattagtt ggtgacattc gtaatgccat accgcaacaa ggacagaatc caccatcgtg
     7201 gcaataataa acctcacacc ttctac
//
LOCUS       DAOR01000305            7585 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_104735, whole genome shotgun sequence.
ACCESSION   DAOR01000305 DAOR01000000
VERSION     DAOR01000305.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_104735"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    471..641
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2323 a         1408 c         1493 g         2190 t
ORIGIN      
        1 gtaaatgcta tctcctctct attatcagct ccgataaatt tcgcatacag atcccttgtc
       61 tgttccattt ttgccttcca ttcatcccat aatgtttttc cgtggtttag tacgtcttgg
      121 taaaattccg aaaatattta tcagatgtag gcgatatatc tatagttggg acaatcaaga
      181 taatatcaac ggggaacagc ggctgattct tgttttaacg cagacgtata ccatcgcaaa
      241 gaataaagaa gaaggcgata gaatgatata ttatgcatcg gtggattcag aaattacgaa
      301 aggaattagg attaccatcg gcgaattttt cacgcctaga tatcgatgaa gacataacaa
      361 aggaaaagtc caataggaca aagaattcag aacatgacaa taaccataag gaaaagtcca
      421 gagtttactg atctcttaca gaggaacctc aataaaagat atgaggaaag nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgaatcag tacatcattt
      661 tgtaagagga gaagcaagtg aatttgttat aaccgatctg gcaaatatgg gtgtttcaca
      721 agccgaatta gaaatggatg ggttgattgc acatccggat attatgagtg agaatgaaaa
      781 aggagaggag aatgagagac taatagaagc tggagtaaaa gcaataggac aaagcgtaat
      841 agtagaacta aaagatacag tcaatggtag gagtcttgac ataactgacc aaaaattcag
      901 atcttacctt agacagctat tatactatct tgttatgaca aacatcgaga aaggaattat
      961 ctctataaga tataatagtc gcgaattaag atgcatcaaa agcagtacag aaggagacta
     1021 tttcttcagg ccatataacg gcgtagttga cttcaaacct ttttcccctc atgatgagtt
     1081 tacagaagac aagatttaat ggcagtgtat gattccataa aaatatcagt gtttcctaga
     1141 atgccctaaa tataagatga tactctatgc ataagacttg tcttgacttg tcaatccagt
     1201 ccacaagatt ctggagcaat tctctcaaga ggccttggtg ttctcatcaa ctaatttgcc
     1261 gtttgcatca aacatatcgt tcgcaaacgt cactatcacc tccggggcgt tgacaggtta
     1321 catgttcagg acacgaagaa ctaacggaga tgatactgag ctcttgcatc ccccagcatt
     1381 ccaatagatg atctcacgat cgccactggc ttgccattgg agtcatcacc gtacggtctt
     1441 gacgcccaat cgatagcatt gtttagtgct tcaggtaccg acaaattata ctctggagtt
     1501 gcaataagaa tgacatctgc tccccttatt ttcgatttaa actctttcac ttttgtaggc
     1561 atatcgctct caagatcctg gtaaggggag gaatgccaga aaggttaaag atttcaagag
     1621 ctgcaccgtc tggcagcaac tcgaccgcgg ctgatagaag tgccttgtca tatgagtcat
     1681 atgaatctgt tctcagactt cctgcaaaat cgagaatttt taacctgttt tccaacttgt
     1741 tttcaccgat atgcttgaaa gttgcttgca acaagcagat tatatccttt ttgtgagccc
     1801 caacagtggc tgtaaccctc tcatccgtca tattatgata ggaatagata gatacccgag
     1861 aaaacaggag aacatatgtg aaagcagcaa gaattgttaa ggtgaatgaa aaactagaga
     1921 tacagcagct tgaaacgcca aagccaagag gttctcaagt tcttgtaaaa gttcaatcat
     1981 cgggcgtttg ccatagcgac attcatttat gggaaggtta ttatgaaggg gtcggcgggc
     2041 agctattaaa aactacagac agaggtgtaa attatcctct tacaccgggg catgaggtcg
     2101 caggaatagt agatagctta ggtgaacagg ctgaaggatt taacaataat gataaagttc
     2161 ttgtctaccc ttggatagga gaaggattgt gtcctgcttg tagaattgga gaagaaaatc
     2221 tatgcgataa acctaggtca ttaggtgtct acatggatgg aggttatgca gagtacgtat
     2281 tggtaccaag ctacaagtat ctggtaaaaa tgagtgatgg aatggatacc gatgcaagtg
     2341 ccactttatc atgttctgca cttacagcat atggtgcagt aaagaatgcc aatctaaagc
     2401 cagatgataa tgtagtgatt gttggggcag gaggattggg cttgatggct atacaactag
     2461 caaaagcagt tactggagct aggataatcg ctatggatct agatgacaaa aaattagaat
     2521 ttgcaaagaa ggaaggtgca gatactactg tcaactcaaa aaatgaagac ccagtaaagg
     2581 ctataatgga attgactgat aagatgggtg cagatgcagt gattgacttt gtcaatgctt
     2641 ctaaaactgt ggaaactgat atgcaatttc tcagaaggag agcccgagta gtacttgttg
     2701 gattatttgg aggtgaattg aaattaagtt tagtcaccat gccaacaaga gcatacaggt
     2761 taatcggatc atatactggg actctaagtg atatgataga gttggtttcg ctagcaacaa
     2821 gaggagtaat caaaccagtt gtttcaaatc gattcaaact tgatcaagct acagacgcac
     2881 tgacaatgtt aaaagaaggc aagattcttg gtagaggtgt aataaatccc tgaaaaaaag
     2941 gagcagcaac agaatttacg acgataaatt ctgcaacaga agaagtcatt aatacatacg
     3001 agataatgac taaagaacag ataaatgaaa agtaactcag gatacatttg gtgagtggac
     3061 aaaggactcg gacaaaagaa ctgatcttct ccacgatttt gctcaggaac tataaaagaa
     3121 caaggaagaa cttgctagga cagtcacaag agaaatggga aaggccatta aagaggccag
     3181 atctgaggta gagcaatgtg catgggtaat ggaatattat gaagataatg ggaaggtctt
     3241 aacaatagac gaagtagtta ataaagatgc tagaaaaact ttcgtaatat ttgaatcatt
     3301 aggagtagta gtgagtataa tgccgtggaa ttttcattat tggcaggatt tgcagctcct
     3361 tcattggttg ggaacacaat cgttttgaaa cctgcaggtg ctactatgca atgtggaatc
     3421 gaaattgaaa aatgttttaa caagattgga acaccagatg gagtttttca aaccttaata
     3481 ggcgactcaa gtattgcaga aaaacttatg attcagcaga tctcaacgct gtaacattta
     3541 cagagtgttc cagttggagc tcaagtggcg caaagggggt aatgatccat tcgtagtatg
     3601 ggaagatgca gatgtaaaag catcaaatgt tgcagtcaac ggtagattca ttaattgcgg
     3661 tcagacttgt attgcatcaa atcggttatc cctatgcctt tggcttattt cttttccgcc
     3721 tttcagcttt ggcatatttt ttgcttcctg atctaatcga actataagca tgagttttat
     3781 tttttttcct atctggtttc ttatgtattg tgcccttacg cattacatgt tctgatacta
     3841 cggaacgata ttatatacta taggatggaa tgtttttctc caagtataat tgtctctgaa
     3901 gctcatgaat taggtgttgc ctttcctcct ccgtcagtca tttggcatgt tccaaattac
     3961 taattctttg tcaaatacct gttctatacg tagggcatca tactagcatg ggaaatggcg
     4021 atggtgggga taattggcat ttcacataat aattagactt cgctctctta attttcctat
     4081 cattttttgg atttcacacc tgttggcaat aattctttgt tagcgagtac ctatttgtat
     4141 gctcatccga aagtctcagc atatgttacg ttatgtacga tacgatagaa acgaatctct
     4201 gaagattaca atactagcta tataagcaac ttccttagca gaacctaatg gttttatcta
     4261 ctataccaag aaaacctatg gctatagagg cagtatccaa agtgcctaaa tatataactt
     4321 cagtatgatt tgttatgacg cacgtgcttt acagactctt caataccgcg cttcgacttc
     4381 cttctatctc aaaagaggaa aaaacaatag gtgttcggtg aatgagctac agagataaga
     4441 ccgatatatc agcgacaatc ctagaagtcg caaacggtgg ggcatctaaa tccaaaataa
     4501 tatcaactgc atttctgaat cactctagca gcctgcaata ttttaggatg cttcttaaga
     4561 ataaattgtt ggtctatcaa gaagaaaaag cgaatgttca agaccactcc tactggcatc
     4621 caaattttta caactatatc atctaatgag tgagttagtt aatgagaata acaacctatc
     4681 tagtattcaa agcgcaaact gacgatgttg gatcgagtaa ccacttcatt atatttattc
     4741 ctcttccaac atacctagtg tgtccacaat ccacaaaatg ctgcctcatt cccctccatc
     4801 tccttgtcaa taaacaagtc caaaaatcct taccatgtac cgcaggtctg ctatggactc
     4861 accctctcta gcagtttcta tcagtaattc caaggcggca ggcgtattta catcattctc
     4921 tatataacta gtgaattcct tggtcagcct tgtcctacga tgagggattt tagtacctat
     4981 gtccttccaa agtgcacttg cgattgtttc gtccgtcgaa tagaatttat caagttcaga
     5041 ttgtgagaac atgaaaggtt gtctgtaatg ctttgaaaag agataaagcc gtataatatt
     5101 tgagttatat tttcttatca gatcccttat tcttatgatg ttgccaaaag atttggacat
     5161 ttttttgccg ttgatgctta ctaatcccac gtgattccag atcttgactg gtagttgatt
     5221 ggatgttaac acctgcagtt gtgccaaaag tgactcgtga tgcggatata ccagctccgt
     5281 tgcaccgcca tgcatatcgt atagcccgtt aaagatggac attgctacag aagcgtcctg
     5341 catatgccac caagggacgc ctactcctaa ggacttgtca taatatttct gaccgaagtc
     5401 atcgatacca ttccaaagca ggatatcatt aggatttttt ttccccggcg cgatgtcaac
     5461 tctgctatta attagatcct cacgactcat ttgtgagagc ttcccgtatg agactagcga
     5521 acttgtatct aagtatatat tgccattaga aaagtaagca cgtttcttac caagaagctc
     5581 tagtattatc tttttagcag tttctacgta atcggaagtc ctggcaatac atagggtatt
     5641 gatacctaat gattgaatat caatcatgag ttcctttata aatctattag aaatgtccgc
     5701 tgcagacgat ctttcctcct ttgcccgtaa cgatatcttg ggatctatat ccgtaatgtt
     5761 taagattgct gtcgttcgct ttccacttaa aattaaatat cttgctacta agtcataaaa
     5821 gagaagtatt cttgcatgcc caagatgaga aaaatcatag actgtcggac cacaaatgag
     5881 catcctcaag ttgccatccc ttgacgttgc cttttcttcc attccagata atgtgttgaa
     5941 tattctcaag aatcgctaat ggtgtacttc taattcatta ttaagttaac atcgcggacc
     6001 tagatcgccg actttgtaaa aaaggtcttc tgaagcttac cataattttc ttgattgtac
     6061 aatacactca taaggtaaag agctccatag ttattacaat agaccactct agggacgacc
     6121 atcaaaacga aggcgattgt gaaagtacaa ctccatcgct ggcatgtcgg gaaatcaaac
     6181 gagaacagtt tgcaaaattt tggtaattca aatgcaggtt catttccagg atgactctgc
     6241 atcgcttatt tcccgttaac tagtcacgga tactgtcagt aatcgttata agttacctct
     6301 gtgttactat acctaaaggg ttctcgtgat catgaactct ggacggaaag agaaggattt
     6361 atccaagaaa ttaatatctc ttgaccttga cgaagggatt aggattgaac atcagtctca
     6421 gcccaagaag ctttttataa acagaaatgc gtgtggtaat tttgttgcgc agtttatcga
     6481 taaagagaag aaccaaaata gtgaaattaa taatatacaa tacttctcgt cagacgttga
     6541 agtattggaa tatgtgaagt ccagatttaa taaaaatttc tccatctcgc tatactagca
     6601 caaatgcgtt atgccatatc aaagaagtgc ggccgccttt cgaataaata gtgaattatt
     6661 cctgacttga cccgaaagcc tatcactacg agacttgtgc tgtacgaccc tgcttgctgc
     6721 catggggtgg tcctaattct gtaaaatcaa taccaacgtt attactcact aagggataaa
     6781 ctcgataatt ggtacttacg atattaagtt taatacggca ttttttccct acccatatcc
     6841 ttcaaagagg tcaaacttta gtttttagaa caacgtactg taaaacccaa aaggcctgcg
     6901 tgaccaagga ttctgtcagc ataagatgat tgcctaaaga aatcgtcaag atacctaatt
     6961 gtagtgagtc ctttatcgaa atcaaatctg tccattgtga atgtagaggt ttgcctgtta
     7021 tcatgggtga gaacatacag atcattcctg atagattgta gtctcactgc cgatctttca
     7081 agatctatgt ccttaatgat tgcagttgga ttgctcccaa ggttgggatt accgtcagac
     7141 acggtcacaa aatctccaac actaaaatcg atgaagttac caaatggtcg gttttctgca
     7201 gaggtcaaaa cgatctcttg attgattcga tcgatttcgg atgaatctaa ttttaagcca
     7261 gtcagggccc atccgtcagc atgtctctct tccgcattcc ttagagacac ataaactttc
     7321 catctgtcag tatctgaaat ttcctcccgt attttccttg agagattgaa tgcagctctt
     7381 cttctggcca ggtcttcaaa ggatagcttg tcgcttcttg caaaatgcca atctaaacaa
     7441 gcatttttta gtggacaagt ctggcagtat cctgttatgg ctggagattc ctgtgctttc
     7501 cataatctga tgaatttatc aattgctaga tagactttag cgaacttgaa tgatctatgc
     7561 ttcacatctt tatctgcaaa ctgcc
//
LOCUS       DAOR01000306           13869 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_29425, whole genome shotgun sequence.
ACCESSION   DAOR01000306 DAOR01000000
VERSION     DAOR01000306.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_29425"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5345..5479
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3837 a         3019 c         2830 g         4048 t
ORIGIN      
        1 acagagatgg gaatgtgagt gaagcgtcgg gagagaatat cttcatcgtc agaaacaaca
       61 aaatatatac accatatata gctggatctg ccttagaagg tattacacgt gacgctgcaa
      121 tagctatagc caagaatctg ggatatgagg taatggagcg tcccatttct cgcaccgaac
      181 tatacatggc atcggagctg tttctcacag ggactgcagc agaaatcgta gctataatcg
      241 atatagatgg tcgcgtagta ggtgatggta aggagggtcc tgtgaccggt agaattcgta
      301 ctacctattc aaaaatcgta acagcagagc tcgcagagta caccaggtgg ctaacatctg
      361 tatggtgaaa acagaggaca aacgaagatt cgcagtaata ggagttggcg gatggggtaa
      421 gaaccacgcg agagtcttgc atgaaattgg cgtcctcgcg tcaatatgtg ataacgatga
      481 attgcgtgca agagaactgg ccacgcttta tgacgcaaag ccttattttt cgctcgagca
      541 attgctagaa aatgaagttc ctttagacgg ctgcatagtc tgcaccccta ccaagacgca
      601 cttcgctatg gcaaaaaaga taatggaaaa gggagttcac gtttttgtcg agaaaccgtt
      661 agcattctcc tcgtttgaat gcgaacagat gatcgaaata gctaagaaaa gaaaagtgat
      721 cttgacgtcc gggtatatcg aaaggttcaa tcccgccatt caggatgtta agcagattat
      781 agataatcac aaatacggtg atctactaat gatggagttt catagagaaa acagaatgcc
      841 cttacatatt cgagacgtcg gcataattta cgatacatcc gtccacgata ttgatactgc
      901 aatgtacctg ttcaatagta cgcctcatgt tgtttttgca cgttccggta caaaatttca
      961 ttcgtatgaa gattttgcga ctattatgct aggctttgac gagcagaggg tcgcaattat
     1021 cgcatccaat tggatcactc cgaaccgagt aagaaagttt agcgcagtct gcaccgaggg
     1081 tatcattacc ggagactata tcactcaaag gatcaaaata gattatgaag aggctacgat
     1141 aataccgcgt cgcggtaaat ttaaagaacc gctaacatta gaattgcaaa gttttctcga
     1201 cgttgtcgcc ggaaaaatca gagaacctgt agtaactcct gaagacgcaa ggaatgttac
     1261 taaggtagcg gaagcagcac ttttgtcgag caacaccggc tcgccgatct atatggattt
     1321 gaaatagaga tgagaaaagc catgcttgat atcttggcgt gtcctatcga caagcattat
     1381 ccactggaac tatttgaact aaatacccgg ctcgaagaag gttccggtac agacacggcc
     1441 ataattgagg gtattttatt ttgcggcaaa tgtcttcgtt tctttccgat tgtggacgaa
     1501 atccccgtaa tgttgccgga cgaattgcgc gaaaggcaat cggaaataga ttttttacag
     1561 aagtggagaa ataaaatccc tgacaaagtt ataaagtccg gtaatccttg gcatctgtag
     1621 aggcgttctt gaatgagcca aaataactcc acttcaacaa taacgaatta catctctaaa
     1681 tctgcaaaaa ttggccagaa cgtcaagatc tggcattttg catacgtagg ggatgatacg
     1741 gaaataggag acaacgtgat gattggttcg ctatcacatg ttgactataa agtaaaaata
     1801 ggcgaaaata ctcgaattga aggatctgtc tatattccac ctttaacaac aatcggcaag
     1861 aatgtcttca tcggacctgg ggcaactttc acaaacgatc cttatcctat gagtcctaaa
     1921 atgagcgggg tcatagtaga agaaggagct atcataggaa gcagagcagt tataaggccc
     1981 ggggtaaggg tgggagaagg tagcgtcgta gcgatggggg cagtggtgac aaaagatgtg
     2041 ccaccaagaa tggttgtgat ggggcaccct gcaagggtga agtattcgag agcagaatac
     2101 gacaagaaaa aagccgagtg gttagcgtcg agttaaatat ttctcgtcat aagtaagttc
     2161 cttactttct aaaccaggat gatttaatac ctaacctgtg gtttcacaaa caggattggc
     2221 cttcatcata ataaaatctg gattctgttt attctttgtc ataattctag cgaaatctaa
     2281 tttagagcac gctactacta tccttgtctt ttcggttgga tatagatcga ataccgacca
     2341 cggctggcgt tccacatctc ctatgtaatc cttcagttta gattcaagag acgataaaag
     2401 ttcaatctta tctccgcgca tctcataggg atctagcgtc catgcgatag atactttggt
     2461 cctgctagcc tttagatccc gtttcttcaa caaatctcgg atctcgtcta ttaaatgttt
     2521 aatgtaacct tcagtcccct ttatgctccc atttaccaga tacatcaatg gtcctgctcc
     2581 tccaagtgag gagcctatgg cccgcagatc ataaaggccg ttggcaatat cgtctatgta
     2641 aaggtcgctg aatgctcgat ttatcggctc gctcttgata ttggtaagtt tgaggttttt
     2701 gcagatctct aacacgcttg ccatatgggc ataatgcccc aagatctgta ttgaatgcat
     2761 aaattccgcg gaaataatat caatgtaatt taaccgaccc ttaatgtcgg gatcgtataa
     2821 catcttggaa atttcagcat cttgtttgga attcaaagac ccaacaatga tttcaggaga
     2881 tgaaatgagc tcttggttga ttggcatgta ataatagaaa atatcttttc cgacactcat
     2941 tccagtaata tgttcaatca aggccatgtt ttcgttattc cctccgattc ctgtaggtag
     3001 agtgtagatc acagaggtgc cttttctgat tgccttagtc gcgtctctaa atttagatgt
     3061 gacatcactt tttacttctt gtcctacttt tcgaatcttt ggtgcaaaga aaatgtatga
     3121 tgcctcactg actgcgacgt ccacgggctc gagcgcaagc agaggttcgt cttctatcag
     3181 cgagcttaca tccgggtagg ttctggcaat atcaggtttt aacgaaatag ccattcgggc
     3241 tgcctcatct atcaatgaaa ccttgaaacc tttaacggct attgaagagg caatgcgata
     3301 tccttctgtg ctcagtccat aaacggctgt cgttatttgc gatgtgctcc cagtcacttc
     3361 gttgcacaag cctccttcga tattataaat ggaattttta ttttctctcc agagctatct
     3421 aaaggacggt taaagaaaag ttttttccat cccatcccac taagtgttgt tatccttgag
     3481 aatttggttc gacatcttga cgccaaagca agtgatgttt tttggtcccg cagtaacttt
     3541 gcttcgtgaa tccgatcacg agttactctg tacgtctaga cattatagag aagccgtaga
     3601 actagccaac gtcaaaaacc tcaaactcaa aattgttggc agacatggcg gcgcagaaag
     3661 ataccaaaag ctgcgagaaa gtgcatctag gatttttgaa ctcgccggtt tgataaataa
     3721 gtttgaacct gatttcgcga tcacattctc atctcctgaa gcatcacggg tggcatatgg
     3781 acttggaatt agacacattg gatttaacga ttctccacac gcagaagcgg tctcgaagct
     3841 cactatccca ctcacctatc atcttttcag tccatgggtg atccccattt ctgcgtggct
     3901 tccgttcggt atttcaaaga caaagataac acagtacagg gctctggatc ctgttgcatg
     3961 gctcaagcgg gatccgtcgc catcgtcatc tgtacctcct gcatgcttac gagaattgaa
     4021 ccttgacccc cggaaaaaaa ttgttctgat taggctcgaa gaaacgaagg catcatacat
     4081 agcagacaag atgttagaaa accgcatttc tatggtggat gccctagtaa ataacttaca
     4141 caattcaatc aatatcgtta ttctatgtag atatgaagaa caaatacgac agttaatgga
     4201 gaggtatgaa gcaagggcct tcatactcag aaacgttgtc gatggaattc ctctgatagc
     4261 ttcagcagac gtgttcatag gggcaggagg aacaatgact tccgaggcct cgcttgttgg
     4321 aacgcctact atttctatct cgccaattcg attttatgta gagaaatatc tcgtgtcttc
     4381 agggctggtg aagagggcat caaattcgat cgaactagta agattgacca acaggatgct
     4441 aaaggatgag aagtataaga aaaaacagaa aagactggcg gcgcgcattt tcgacaaaat
     4501 ggaagatccc ttagacaaaa tgctctcgta tctgaacata agcacgcaac attctaaaga
     4561 tggtaatata tagggaaaaa tacgcccgga agcccggtag aggactgaca gttctgccga
     4621 ttgtagtggc caagcataga ggcctttgga gcctttgacc ccagttcgaa tctgggccgg
     4681 gctattcttg tgaatcactt tttttgatag cttcaacgaa gccgcgcagt tatgatgacc
     4741 gaactgatga atcgctccca actcttacct atctgggatt ttttttgtcc gaccgaccca
     4801 tcccctgaaa gtcatttctg catgtcagaa aagggattac tatgtaaatg acccgcggac
     4861 aactctgtta ttgctaccat cttgccggca tgctccaaca atctaataat acgcttattt
     4921 tcttcaggcc ttttttgaat aattattcac cattctcgtg agatttttga atgcttcgtc
     4981 cacaagtccc ttacgttttg cgattcgctt cgaatagtcc gacaatcttc ttctcgaaag
     5041 tttaaccata ttctgctcct gcattggatt tggggaacct aaagattgtc gtgaactaat
     5101 tgatttttgt ggagcggctt cctttatgtc cttcaacact tcttctgccg ataacttaga
     5161 ccccaagctt tcaagcactt ccctgatatc ttcctctcga agcattcgga ggggttgtct
     5221 attattacct atcgcttttt gcaccagtaa ccccatcact ttatgggcgg ttcggaaaga
     5281 tatcccattt ttgatagcca atagttctgc gagatcgaat gagatagcat agctgctact
     5341 ggaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnna gatagcatag ctgctactgg atgcatgctg catcctttcc
     5521 ttgtttatga tcagcgattt cacaacataa ttcatgattt tcagtgcctg agtcgtcgta
     5581 gcagaggctt gccataatga tgtttttatc tcctgcatat ccctgctata tcctgatgga
     5641 atagccttga taatggtgat cattgtaagc aaattaccag acactgttcc tgcctttgct
     5701 cttagtagtt cgagaggatc aggatttttt ttgtgaggca ttgcactgga tgatgagcta
     5761 aattcatctg aaatatccag ataaccgaac tcatcggtcg accagattat aaaatcttct
     5821 gtcattctgc ttaatgtaag cattataatg gtgagttcca tcgtatattc gataattgta
     5881 tcacgcgaac tagtagcgtc gatggaattt cttacgattc ccttaaatcc aagcaatctg
     5941 gcagtcttct gcctgtcaag ttttatcctt gaccctccta tagcaccggc accgagcgga
     6001 gattgattaa tccttacgta agtaatgtac agcctgtcca tatccctaaa gagagaatct
     6061 gcgtacgata atgcgaaatg tgaaaaggta ccgatttgtg cctgtcgtag atgggtatac
     6121 attggcataa tagtgtcaat attttcggct gcgtttttaa ggagtgaagt aattagagta
     6181 attatcttct cgcatatttc agttatgtca tctcggacct tcatacgaat gtctaacata
     6241 acctgatcat tccttgagcg accgatctgt attttcccgc caatgtcgac gccggtttgt
     6301 tgtatgataa gagattcgat agactcgtgg atatcttcga catctccagt agctagcatc
     6361 gccggtttgt gcctgatttg ttccaatgcc accaaaatct tttttgtttc atgcaaagtc
     6421 aatattccga tatcatgtag cattatgaca tgcgcttcac ttcccagtat gtcataatgt
     6481 aatatttgct gatcgtcatt cagggaagac agaaaattta acatatcttt gtcaagattg
     6541 cccttcggcc ttgaacgata cacacattgt caacatcgac ggtttatttt aatattataa
     6601 tagttaaagc agaattatgt tacttgttat tggtaacgcg caataggagg tagacaacgt
     6661 cgagatgttt gaaatacaaa ataggcagtt cgtatgggtc gactattttg ctatgcaata
     6721 gttatatatg gatcacgaga aaaattaatc gtaatgagcc aagatattca gcagacgcgt
     6781 aggcaaatat tcgatgaatt aaccaagata gtggaccctg aaataggcgt ttccattatg
     6841 gaattggagt tggtcgacaa ggttgatatc aataaaggca acatagatat agatctgcat
     6901 ttgacaagtc ctttctgtcc tgccgtattt ggtttcaaga tagcacagga cgtccgggat
     6961 aatgtgtaca gattgcctgg tattgaagag gtcaaagtaa gtgtcagcaa ccactttatg
     7021 gcggatgcaa taaacaaaca agtaaatgaa agtacgcctc cagcaaaagc ctagaatcta
     7081 ccgcattcta ggttaggtta tttggttgcc gtggctgtga tgtttcagct ctgcgatttt
     7141 cttattatgt tttgaaaagc tcatcatatg acccctaagt agttgtatcc ctaaagctgg
     7201 gtctactcct tttgggcata cgctgctgca ggacccagcg aaatggcatc tccatattcc
     7261 gtgtttgtca tcaatagctt tcatatcttc acgtgaacca atatctctgg tatctgccga
     7321 atatctataa gcttgcacca aggcttgcgg ccctgggaat ttcgtgtcgg tcgccaccgt
     7381 ggggcaagct gagtaacata agccacattt gatgcaattt gaggactgca gatattcgtc
     7441 tacgtctcgt ggactctgta tgaattctga caattcctta ctatctcgaa tatctgcgtt
     7501 ctgatttatt atatagggcc ttacactctt gtgatggcta aaaaattggt aaaagtcggt
     7561 tactaggtct cgtataagtg ggaagtttgt caagggctca cagctgatta ccccggattc
     7621 caattctgat acgtttgtat agcatgcaag tcgaggtatt ccattaattt tcattccgca
     7681 agagccgcat gatgccattc tgcaagagta cctgatgcct atgctagtgt ccttgtaaga
     7741 ctttgcatat agaagagcgt cgagtactgt tgtccagcgc tgaatgggca cctcaaatgt
     7801 atcatagtga gcggaatctc caacggcctt gttcgctcta tatactctca gcactatact
     7861 actgcgggct ctcaatgact tcttacttct ctcgagcttg ttacctgttc ttaacataga
     7921 tatttttttt atgataccac tcatttcatt ctactccaag tccattagcg ataattatcg
     7981 ttctagtacc gaacgcaatt atggctatca ttgcagcaat cgtagatatc gttacaccat
     8041 tttcccatgt cctattctgt cgtaattcta gcaatataat tcgtaggcca ttgaagccat
     8101 ggatagcgag gagcacaagt atcgattcca gtagaaccac atatggcaag ttatggtaat
     8161 ttgcaactac gtttgagtac tgcaggctca tatggaatgg cattatcaaa cgcattagaa
     8221 tatgaactgc gactacgaat aacgccgcta gtcctgtgat gtaatgtatc ctcattattt
     8281 gtctttcgcg cagcatattt ccatcacact atattctgat tttcttttac tctccgccga
     8341 agagcacgtt accgccatag aacattgcga ccgctgccag gataagtgca atccatattc
     8401 cggatttctg ccggtaattc agtgagagag cgtcatatgg atagtcaggt cttccaggtc
     8461 gtccgagccc ccttccggct tccgtaaata tcaatctaat gccatttgta gaatgataag
     8521 tacttgtccc gataacaaga atcagaaaga gatgtcccca aggcgtttct gtaaactgta
     8581 acatcgcgtt ccaagcgcct gcaccgccgg taagtgagct tgtctcgtat atatgcccaa
     8641 tgaagtatat caaaagaaat aggcccgtta atcgctgtaa ccagtaagac acacgctccc
     8701 agccatacct cgtcggatta agccacccct ttaatccctc acggttgttt cggtcctcct
     8761 cagctgtctt gaaaagtccc cttcggtttg atgtgcgcat tagtattttc tctccgtagg
     8821 agcataacgg gtaatagtga ttggatgcca ggcgattttt ggctgcatgc cataatatgc
     8881 caaggtgtgt ttcaggaagt tcacgtcatc cctcctagga taatcagtcc gtgcgtgtgc
     8941 cccccttgat tcttgtctac tcaaagcccc cattagcaca atctctgcta tccttaacat
     9001 agaatctatc tccatcacat ttataaaatt agtgttgtat tcttttgctt tgtcgtctac
     9061 atgcttccac gacgagctct tcagtgccat tacttttctt aagccttctt gcaaaccttc
     9121 ttcatccctg aagacatggg cctttgcatc cataatatct gtcagatctc ttctaagttg
     9181 atatggattc acttccccgc ttcctctgaa aataccatca taaattctct tttcttcttc
     9241 caagacctgc tgtggagata cagatataga tacagaggca gctttactgc tagatgcgta
     9301 tcgcgcagca agttcgccag taattcgacc ccaaacaaga cactctgaag ttgagtttgc
     9361 accaagccgg tttgctccat gaacactatt acaagccacc tcccctgcag cccacaatcc
     9421 tctcatctca gtagccccat cgatattagt gtgaattccg cccatcatat aatggcaaac
     9481 tggtgtgatc tcaattggat catcaattat gtcaacccca gaaaatttaa ttccaatgtc
     9541 cctaattcct gccagtcgtt cttttatacg gtcagcccct agatgggtaa gatcaagttt
     9601 taggcagtcc gcaccggatt catgtttaaa gcctctgcct tgctgaatct cccggatcat
     9661 agcacgagaa acgatatctc tagacgccag ttcaagtttt tctggtgcat actttttcat
     9721 aaaacgctcg cctccagaat tgataagata tccaccttcg cctcgcgcgc cttctgtaat
     9781 tagtatacca gaaggtaaaa ttcctgttgg atggaactga acgaattcca tgtccttaag
     9841 gcctattcct gcacggtagg ccatatccaa accatcaggc gtagaagaat atgcataagt
     9901 tgagaaactg tatatcctgc ctgctccgcc cgtgcaaatt atgccggcag gagccaatag
     9961 tttaaaaaag ttcccagttt tcatttctat agcagtgact ccccggaaac tattttcatc
    10021 actcagaatg gaggtgcaaa accactcatt ataaaaacgg atattgtcaa atttaagaga
    10081 tgtgtcatag agcgtttgca tctcgtaaaa tcctaccttg tcttgagcat aagttgctct
    10141 tgggaatgag taacctccga aattgcgctg gtcgattctc ccatcatttc tcctagacca
    10201 cggcattccc caatggtcta gttgataaat ttcgtaaggc gtatcatgta ccagtcgttc
    10261 agcaacgtcc tgatcggcaa gaaagtcact gcccttaata gtgtcgtaga tgtgagattc
    10321 ttgattgtct ccttcttcct cgaacagtac agccgcagtt ccgccttctg cagacacaga
    10381 gtgagatcgc atgacctgga ccttagagac cactgcgatc tccaatttct catttgtagc
    10441 ggctgaagaa atagccgctc tcaacccggc tagaccggag cctataatta atacgtcctg
    10501 agagatagaa tcgaccaacc gttcttgcca gctgctacgg gaggtaaagc aatctcataa
    10561 atatctttgt gaccaaaaat aagataaaaa atgaattctt taggttatac tcagtaattg
    10621 ttacttggtt cgatatgaga aaattttcgc aaatcttcag aaggggaaat aaaaattagt
    10681 tgagaagctg gacggacaaa cacacacaat ctccaaggtg gcctacatgg cgattttgta
    10741 gcgaatctcc gacttacgta ctacgtgctg tttcacacgt acgataacga agcagcatgg
    10801 aatcgaaaac ttatgatata gagtcaattt ctttcagaag accccaataa gattcacttg
    10861 ccatgctgga acactctaca gactgcttcc tgcagatggt tcacatttaa tttggtttcg
    10921 gtacggtcct tcaggtcctt tagcgttaca agtccatttt ctatttcccc ttggacaacg
    10981 agaactatca aaagtgagtt tttagtgtat gcgtcttcta gttgtcttcg aatagacctt
    11041 ccctgaatgt cataatcggt ataaatgccg gattttctaa gatcagatac tatttccaga
    11101 acatgttttc taacactaag cgatgtgtgg gcaacgtaga ctatcggagt ttttagtggc
    11161 tccaaaagtc cacgtttttg catcgctagc gctattcttt caactccacc agcggcaccc
    11221 gaggcaccga cgtccttcct tccgaaggcg tccgtcaaga tgtcatatct ccccccaccg
    11281 actagtgcac caacatccac aagaggatcg agtgcctcaa agactattcc ggaataatag
    11341 tctagccctc gcacaactcc caagttgatc ctagtgtcgt ttactttcct tgacttcaaa
    11401 gaatccatta actcttctaa ttttcgccag cttatcaggc cttttgtttc aagatttgac
    11461 ttaacctctt caagactacc cttgatccta gaaagatcta gtaagctgtg taactgagta
    11521 gggtctattg tgcttgagta ttctttgaga acttcttcga ggcccttctt cggaactttg
    11581 tccagagctc tcagcatttc acggataatc ttttgatcat aaaccccgag cactctttga
    11641 atatattgct cagtcaactg aagatcattt atctcaatga tgaccttcaa tcccagcttc
    11701 ttgagaaaga tcgaaacaaa ttctattact tccgcgtcgg attccaagct aaaggagcca
    11761 taaatctcta tatcccattg gtggaaatac ctatatcgtg ctgcctgtgg ctcgtcgtat
    11821 ctccaaacgc cagcaaatgc agcaatttta gccggcattt tcaaatcccg tctcgaagca
    11881 ataaagcgtg ttaatccaat ggtaaggtca aaccttaaag caatatttcg cccccctttg
    11941 tcagtaaaac tgtatatgtc gttggatatc gtagggccgg cctttgcctc aagggtggca
    12001 agcatttcga ccggagaggg ctctatactt ttaaagttga atagcctcgt agtctcaaag
    12061 aatttctctt tgatgtaagt gattccaagg agctcgtccg attcgaggtc tctcatccct
    12121 cgtggaagct ctaacttcat actcaagaaa attcggggtg ggcatttaga ttttgcgatc
    12181 aattataagg atataaggca aaattgggca atatgacagg ctttcgctac ttagatcaca
    12241 tgactgacgt tatcgttgag gcgtatggga atacactgga agatgccttt gcaaattctg
    12301 cccggggcct cgtcaatacg atgttcgaat tatctgaagt cattcccgat atggaagtcg
    12361 ggatctacgc cgagggacat gaccttaaga gtttgcttta caactggcta gagaaggtca
    12421 tgctaataat gctaatagac aatatcgtcg tttcagattc aaaagttatc atatctgaat
    12481 gtaatgatag atattcgatc aacgcagtcg caaagggaga acgtatcgac ttagagaagc
    12541 accattacaa aatagagatt aaagcggtaa catatcatga gatggaaatc aagcaagatt
    12601 gcaaagatgt gactattcgc tttttgctgg acctttgaca ttgcaacgtc gcggctacaa
    12661 aagtgaccaa atagtcatac tcggacaatt gattcatgac aaaatcttaa atagcaaatt
    12721 gctgctttac tttttgtgta atgagtgagg aaaaaaaagg taatagtatg caagctctaa
    12781 tggagctttc tgagaaaaat tttgatgaaa tagtgagtgc aaccaaaccc gtgttggtgg
    12841 atttttgggc gacttggtgc gggccatgtc aattcatgct acctattttc gataagttag
    12901 ccaaaaagta tctcgacaaa gtaatgttcg caaggttgaa tgtcgacgac aatcaagggg
    12961 ttgccatgaa atttgacgta tatgctattc ctacatttat tattttcatg aatggcaagg
    13021 caatagatag ggcagtgggc gcagtcggag aaaaaggctt agagggatta ctacaaaaat
    13081 accaatagtc tctcacaagt tgtctggttt taagttagtc gattcccgag tttataagta
    13141 accgaaggcc tgatcttcgt ttcttttcag cgataaaatt ttgccaaaaa catcttcctc
    13201 ttctgggttc agtcgtacct taaaacgttc taagagcagc tttgcctcct tgctgttaag
    13261 atcagtatag aaaacatctc cttcgttaat ttgtcttcca attactggcc ctctaatgga
    13321 gactgcaatc tgcatcccct tagttgcttc ttctatcgat ttgccatttt cctggacttg
    13381 atgtattatg cctatcttct tgcctccgtc atttatcact cgcactttct gtctaagctt
    13441 cccgactaga acttctgccc cgaatacggc agggtcgttc cgtctaaata tgaacccctt
    13501 caaaaattgg aatttacaaa ccggtgctat ttcgttaaat aaaatggaat cttcatgctc
    13561 tttttggaac ttaatccagt ctgtatatga tctcactaaa ttgtatatta ttcgatcatt
    13621 aacgatctta acatttcgtt cctgcgattc tttaagtgct tcttctaaaa tttttacatt
    13681 aaatccaaga accacgccca tgtatctgtc cttctctcgt actgcagatg ctgcaattac
    13741 atcccttctg gtaataggcc caatatcagc gctctggatc gtaatgttct cccttttgag
    13801 gaggtccgta accgcttcta tagaaccaat cgtatcacat tttattataa ctccgttaga
    13861 attagtgtc
//
LOCUS       DAOR01000307            5529 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_63037, whole genome shotgun sequence.
ACCESSION   DAOR01000307 DAOR01000000
VERSION     DAOR01000307.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_63037"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1648 a         1122 c          989 g         1770 t
ORIGIN      
        1 atcgattgga cttattggac ttattggact tattggactt attggagtga agggtaaggc
       61 tttacttcta caccaactta ttccgcttac agccacttgt gtggtatcag tttgtttcct
      121 cgatccggtt taatttagct taatattcaa aataataagt taaatataac aggcgttata
      181 acccaaatat atacaatagt tacatgatgt gtgtcattaa attgaaacat ttacatatat
      241 aggcacccaa agtcaccctt agtgatacca agttcggaag caaatgatat tggcaatata
      301 ccactagact tctcttacgt gttaaggacg tattcaatat ggttatggat tacatcttaa
      361 tcaaaaagtc atttatgata tgcactacta gtatacatat cataaattca tacatatttc
      421 attctccttc ctcagacaga aatctcactt tattatttcg gcaagtttta aggctatatc
      481 ttgcatatcc ttgttgtttg ctagtttgtt tctgagtctg tcttcaatct ctagtttttt
      541 ctctttaaca tcaataaaac ccttaacgtt acccaattca gatagtattt tgtcctcttc
      601 gtcttgtgtt actgtctcag agacaaatgc gaggtgggat aagaatttct gtatcatatc
      661 gggatagatg tcgtatgtgc ttggtagtga aataacacga ttatagtttc tagtaggaaa
      721 taatttctct aatttataaa ctataaattc ccaaagtctc tcgccgccaa ctgctgcaac
      781 gatgctatct atttctatac gcttatgttt tacgtattca aggtcaatat acttgtatct
      841 ttgtaaatca gattccacca attttttaac agggtttatc tcctgactct ttggcctata
      901 ttcttcttcc acatcttctc ttgatatgtg caaatactct agtgcatcat catcgtcaat
      961 tcttatcctc ggtatctctt ctggtgcttc cgcggcaata gtaattccac ttgcatcgcc
     1021 atcggttata attacagcat gtgcacctgc ttccttggac gctttcacta aatctttacc
     1081 gtattctgtc aaatgtcctt gagtattcac taatgccatg ccacatttat cggcaaaact
     1141 ggcaaacact tcgggcactc cctgcttctc tataaacagg atatcggttc ctttttctgc
     1201 aaggctttca atgtagtcca ggttgacact tgtccatctg ctatcaaaat acatttgtgc
     1261 tctagcagaa gcaattattc caagcgcttc acgtgaaaca ccaaactctt ctttgcaaat
     1321 atcattgatt atgcttgtaa tatagcttct agttccttgg tttatttcaa taccgttttc
     1381 tataagtgta ggccgtaagc gataccaaac agttgattga gtccatctat catcgatttt
     1441 taaatctgaa tctatcttaa ctgcttccca tctttcacgc agcaattcta ttagaaggtc
     1501 tttcttactg tctttgctct ccttatactg ccattgtcta tcaccataac cataaccacc
     1561 atgaccatga aatgaaggca ttgatgacgc tatactgcca gttgtctccg caattgtccg
     1621 ggcaaaaggt tgtaaatcaa tgcgagattt tcccataccc atccaagaaa tcgcaggaca
     1681 ttccaggtta gcaataaata tgcatggcgc tttttgcttt gatataggta aatacctatt
     1741 gaacccacaa ctgtctaaga tctcactaat acttgatgct gattttgtat atcctgcttt
     1801 tttgtccaac caactatagt taccaccttg gaaataccca tgtccgtcat ctgcggaaat
     1861 actagaattg atactgccaa cgaaatcaaa ttgccctccc tgtgaaaggt aagaatcctc
     1921 atatggagct gctgcaatct caaaaacata tggataattt aatgacccat cgtcataata
     1981 tccggtaacg attttgtatt ttgctttgtt tcgattaata ttgtattgac cggcaagatt
     2041 gtcgatcagt tcttcccctc gttctgcttt actactgtat ggtaatgcga gtttggactg
     2101 tggccgcata ccatttctca ggctattata aagctcttca atttgtttat ggtcgttctg
     2161 tcttaattct gctactgtca tttgtttgaa tcggtttggt aaacacttta cctctctgaa
     2221 gtcttgtaag gcatcagata caacgctcga atggtctgca agacctaaaa taaagtcctt
     2281 gaatttagcg ggcctgtaat aataaatgct agatgaatta tcccaaccta ctgtcattct
     2341 acatcattcc aaccgaaatg aaattcaatt ttattacgaa ataaggtgcg ttttttgcag
     2401 tattctttaa gacgtaatat tatactataa ccggattcgt ttcctactat aggtaatgct
     2461 gcttcaacct ctacgtaatt gtttgctgct actggaggac cgtgtattaa cctgatactt
     2521 ctatcttgct cggccttatt aactcgtatg taaactttgt actcttttcc attaaaccta
     2581 aaaattaaag gcttatccca ttgtttatcc ttaaatgaat ctcccgtgtc cgtgttagtc
     2641 accagtgcgt atcccattgc tagtatttcc ttcagggcgt caccttgagc gccgcaacta
     2701 attttttgta tattccgctt tgaactatag aagtagtcat aatcaactac ggcccttaga
     2761 tctggaaatg gtattatatt acggtcgtta gaatttccta ctactatacg aagtatggca
     2821 tcctctttta gttttaggtt caccgtaata atcctggcat ctttaggatt cttgtagttg
     2881 ttattcagga aatcaacggc attatccagt aactctttaa tacagaatat aggaaaatcc
     2941 tgctgtttgt aaccgattcg ctgctcaata ccgctaactg tgaaataacc catactctgt
     3001 tttgtagaag aaaaaacctc atgagataga gggggtatgc ctttttgtcc gtttttgttg
     3061 cttttagact catgagtctt ttcggtcatg gaagttggac cccccttctt atttcctgct
     3121 atcattacta tctacatgac gacactgctt cctgtcgttt acgtcgttat gatgacactc
     3181 gcagatgcaa acaaatctat gacctggtat ctcatttctg tacgaacccc tgcaatcttt
     3241 acaatcattc gttaggcaac atgtgacaaa atcttctata caaataatcg aactttttat
     3301 taatgacgtt ttgtaagaat tgttgttatc catcgtggaa ccacttcact ggggggaaac
     3361 tccaacacgc tgttgcttct cccaagaaga ctgaccagtg taggactttt atttgttgta
     3421 atttgcatga tatcaagtca tcaaacgaat cacctgcaaa atccgtcttt tttaagatgg
     3481 atttgtttat aacagggaac ttgggttcat tcacagtagt atcgacctcc ttggttagat
     3541 gctatgactg tgttggtgaa agtcctgcaa ggggcgggaa ctccagaaca ggacttcacc
     3601 atgcattcaa gtttattcaa cttttatctg ccattccgtt attacgggat gctgaattaa
     3661 tttaaaaata ttagcttctt gaaaaataat tcgctcccaa aatttactcc tgagaggtaa
     3721 acctatgcta acattattac ttactaatag ataaactcga taatctgacc ttacgattta
     3781 agtttaatac ggcatttttt cctaccctgg tcttttgggt aggtcaaaat gtgattttta
     3841 acagctgtca ctatgaaact aaataggctc tattcgcacg ctcataggtg acattacttt
     3901 ttaaataatt tcataaaaat atacaaaaat aaagtgtaac gccctcccgg gagggtacgt
     3961 cggccttcta agcaagttgt atgcaagtaa tacacggagg taaaacatat tattaagtat
     4021 ctccagacct tttaatcgat caaaaaactg gctttttgag aggcttcgct ccgcaactac
     4081 ttaaggagtt ggtagaggta gattatggac tttttcccca tagaagcatg aaaatgttcc
     4141 ctagcactcg attttttgag tgtttgacgc atggacagaa gcaaatgggc cgcacagcag
     4201 caacttctgc gctggattcg tagacggata caaggcacaa tggacaagcc tagcaccggg
     4261 cattatgcag agaatacaac aatcgacaac tcagagcagc ggcataaaca tcaaaggcag
     4321 caataacgat gtagccgtga atcagcaggt taacaacaac atcggaggtc agaacggata
     4381 tggcagcggc agcggatacg gacatagcag cccccccagc tctttttcag atattgttgt
     4441 tttaatggac atgccagaca tacatcttcc atctcagtta caatcacgac tgctagaata
     4501 cgttagatgt gtggtaaggg aagaaaatga gaacattcag ttcattattg taacaaattc
     4561 atctgctttg atggataaag ccactgctga agaacttttc attttgatgc catcgcagca
     4621 gctcgcggag tgttccaatc aattagtgaa actttctgat gcgactatgt gcacaatgcc
     4681 tttgtgatct cagaatgatt tcaattgaac ttagagaaac cgatctgcaa tccatctagc
     4741 cactagacgg cgaattatgt ctaattaagg gaattgaaaa catctgtatg agaattccca
     4801 caatttggtc tgttaggttg aaagatgccc caagaaattg attcagaaaa ttactggcca
     4861 gatagtaata gttgtgcaca aacccagaag gaattagaag actttaggtt accagagtta
     4921 gcggaagatc cctctagaat cgattgtgaa cctgatcttg gagaagaaca agaagagtac
     4981 ttcagacgaa tatgtcaggt ttcgtagatc ctagatccta tgaatagaaa atggaaaaca
     5041 aggaactcgg acgaatactt gagaaaagta ttgtcagaaa ctaagactca tagataatac
     5101 ttttcatata tcacagatga aatagcctca agatatttta gttgggacga gtaatatgat
     5161 agaataacaa gagagccgaa atgaaaggac gatacaggta caggaacaag aaaaacagcg
     5221 atcataaatt ccgtagatct ggtcaatcga cactgttgaa acaggaaaag gtattcagaa
     5281 aatgacaata accataagaa gaaatccaga gttcactgac cttttacaga agaacctcaa
     5341 tagaagatat gaggaaagac gaagatttgg tgattcaagt attcacgttt cagatatcct
     5401 acctactaat tgcattagaa aacagtatta ttcacgtaag tttccagaaa tcgaccctat
     5461 ctcaaaagaa tcagtacatc attttgtaag aggagaagca agtgaatttg ttataaccga
     5521 tctggcaaa
//
LOCUS       DAOR01000308            5662 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4529, whole genome shotgun sequence.
ACCESSION   DAOR01000308 DAOR01000000
VERSION     DAOR01000308.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4529"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2884..3017
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3338..3559
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1745 a          907 c         1004 g         1650 t
ORIGIN      
        1 gtaggacaac tagtagcttc aacatataaa gatatattca caatcgtttc actggtagct
       61 tggaacattt gatatccagc aacaattcat tcttccattg gttcgttgct aaaggtattg
      121 tcttttgaag ttcgggatat atgttcactc aaaaagacca aagatttctc taaatgatat
      181 attgaaagta gttcaatctg ctggccttgc ctattcaaac aggaatccag acatggcaat
      241 agtggttgga ggagatgcac ttttggctat tacggaaaaa aacttcggat tcctatgctc
      301 tttgtaggtg taaatgataa agatataata ggcagtaaag cgaggcttgc agaagtttta
      361 tttgaagatc ttcccaaagc attagatgac atcaataatg gtaattacag gttagacaaa
      421 agaaggatgt tttctgtatc tatcaaacat aaaaataatg ataaccagaa tggtatcgaa
      481 ggagcgatag acatcttgac ggatatatat attgaacgtg gtattttctc acgttgtata
      541 agatatgcct tgtcagttac catacgcgaa gcaaactcag aatatcatac actgaaaaaa
      601 tttgcagagt atgccatagg aaatggtata attattagca cgtcgtttgg agcacgtgga
      661 tattattctt atctagacag aataacagca ggaaaacgga acaataataa taaaagtaat
      721 aataatagca ttcaaacatt tcctgacaat agattaggta tctgccatat catacccaca
      781 tttttagtcc gaagactgag tctagcgaaa cgaaaggaga ataaaaagaa aaggacactg
      841 gttccaagta gctacattcg ttatactatt ccataccaat caataataaa aattagtctt
      901 accagaaatg cagatgtacg tctttatgga actactgaac attcaagagg attagcaatt
      961 acaagtaacg atgaaataat gattagccca tctagacgta ctgcaaaaat tattcgattg
     1021 gattatggca gaacataact ctgactcatt attataagat actgaaaaag cagataattt
     1081 atcaccgaca atatatgtgc aagatagttg acggtttaga gtcggatacc agaccatccc
     1141 cgtttccatg tggttcttga aataccacta ttgaaagatc ataagtatct ataaatacaa
     1201 aatctatata tcatacggtc gtgtattata atggcaaata ctacgaaatt tctgaagaat
     1261 agattgtcga atcattcttc aactgcaaat tcaatcacta cgtacataac caatgttagt
     1321 actatgagca agtcaaccgc acgtgaatac tttgcaaggt tgaatgattt caaggagttt
     1381 atagcaaagg agtacaacta tcgcttaagc atagatgacc ttcttgttaa ggtaaagaaa
     1441 gcccaccagg atccatacaa tcttctgaat ggttatgcct cgtatttgag gaatcgtaat
     1501 atttctgcac tcacattaaa gcagcgcatt gtgacagtga agaacttttt tgaatattgt
     1561 gatattgata taagcccacg taggtttaag ttaaaggtga aactccctaa agtagttaga
     1621 aaaaagaaag aagcgctatc aaaggaagat attgttgata tattgaatgt gtgtgacaat
     1681 attcgattaa ggacttatgt aatcttgcta gcggctactg ggatgcgtgc tgttgaagca
     1741 ttatcgattc gtattaaaga tatagatttt gattcgattc ctgttaagct ttttgtgaga
     1801 ggagaatata ccaaaactaa agttgataga atcatatttt tgacagaaga agtaactcaa
     1861 caattaaagt cattattaga ttataaacat agaactcgac gagtatgcca tcaagataag
     1921 caaggggaaa aaccattaca gaatatataa taccagataa aaaggatact gacttggtct
     1981 ttgcagttta tcaaagcaga aacgcaccga atcctgatat cttatatgat gatttgggta
     2041 gatcatttgc aaagacactc gatcgttcag gtaaaggtga cagggaggat ggtaatgaaa
     2101 ggcgtcgaca gataagcctt cactctttta gacgattcgt gaaaactaca atatcagatc
     2161 ttggatatgc tgacttttct gaatggttca taggacattc tggttctaca tactggacaa
     2221 aaaaagattc agagaaagca gaactattca aaaagattga gccatatctt acctaccttg
     2281 actatcctgc tcttgagcga aaaggtgccg acgtacagag caaggtagaa gaattagaag
     2341 agctgaatca atcactgaga catcatgata agatgaaaga tgatgctatt gcgcatttgt
     2401 cagatcaaat cctagctcta acgtcaagat tacaactaat tgagaaaaaa cagatgcaat
     2461 aacataatct aaattattct aagcctattg caacttatcg aatctaagct gagttgtaag
     2521 acaaaagact tatcttattt cactcctagt gacatgtata actcaggata gcataaaata
     2581 gtttgactct tcgtgcttcg tcatgatatt gcttgtaggt tgattgctga atgtgaaatt
     2641 tcatatgtta aaaacttgtc acagccacaa gcacctacta acagagtata gcaaaaacca
     2701 taacaactgg tttatcttaa tcggtttgag tattttctct ctcatgtcgt gttagattgc
     2761 aagtcattct gctaatgctt tagcgtcttt aaaaacggcg aaattctttg tctccttatt
     2821 tagccaagtt ggaattattg aaagtgtgat aaagaatgta tccggcccaa ggaaacgatg
     2881 tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnatt tagccaagtt ggaattattg ataaaccatt tggtttacca
     3061 catcttttac atttaattct ttgaatatca tagatcccct gagttgtcga tttgaatgta
     3121 cgtcgcagag atatgaactc aaataaatcg aatacaaatg gatatgcctt gcacgttcta
     3181 caagcgccta ggagcgtaac cttcgaatga ttattaatgt tattgtaccg cattagttcc
     3241 cagtcttcat ttatatgcca aatttcattt gactctacat ccaatttaaa ttgaaaaagt
     3301 aggagtttct gactctgttc gctcatttcc atcaacgnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnna aattgttcaa agatcctcca gctaaaaata gctgtctggc
     3601 tttgcgagga tttttttcga tacacaatga aaggagaaat tctttgttat ctaataatgt
     3661 agagcgaatt tcctcagagt ctccattctt gctgataaag tcaagcataa aacgtccaaa
     3721 agaggatata tcatagaatc gaaattgagc gagcaaggta cccaaaagca ttgtgatggc
     3781 ctttgaacgg tattcacctg tcctagaata agcttcgatc aaccatgcaa taaaagtgcc
     3841 ctccactgtg aaatagtata cattcttcct tgtgtcattc tttctcgatt tttcctttcc
     3901 ttctatatta attaacttga gcgaaatcag atcctcgatt ctatctttga tgtaagtccg
     3961 tttagtgtgt aatctgtagc tgtttcgggt gtgcgatttt gagtctgtat aatagtccac
     4021 aaatggttga tggttcttta ttaaccaatt tcctatttcg gttatatcga attgtttaag
     4081 tggctggcct ccttcaaatt ttattgtaca gtacctaaga atatctctgc ataagggagc
     4141 atgtttggat gacaaatcta taattgattg gatactaact agatcagaca tatgcctaaa
     4201 tagtaattag tatctaaact aaatgtctat taaagttttc atatactaat taattaatga
     4261 ccggcgatct acaatatcgt aaagttcaag gactcatagg cgaaacttct ttttccataa
     4321 ttttgcctaa ggcctatgcg ataaatctag gaattgcgaa gggtgatttt gttaaggttc
     4381 gtcaagaaga aaacaggata gttatcgaaa aggcgtaggg taaagaaaaa tatgttaagc
     4441 atgctatatt cctgcaaact agtaattcca agaactaatt ccaaaaatca ggactccgtt
     4501 ggcggcaaat ttggaagcat tcaatcaaat cctacaacga ctggtcagtc tgtatttgca
     4561 aatgaaacca attgatgaat ccaatggcaa agtcaataat caattttagg gcacttaaat
     4621 ctgtgtatag tcagggctgt tgtctaattg gctaccaacc ttatatataa aaaaagtaga
     4681 agatacggtt atggctttag atcatagata tagagacaca ttaggtgata tgcgatttga
     4741 cgctttggtt gctacgcaaa cgactgatag aggagcatta ttacattatg tttacgcaga
     4801 acgcgtgagt gagcctatac ccaattatag agtgctgggt tctggcgaac catatgggac
     4861 tattttccta aatcgatttt gtaagaaaga gaaagaaaaa acaatggaag aaattgcaac
     4921 aatagggtat ttcataatca tgtatatcga aagattcgaa ttaaatgata aagtgggtgg
     4981 tgaaccacaa atttggttta taccagatac cggtcaactt gatactccct cggctaatca
     5041 gtataagaca cttaaggaaa atacagacga aaggttggtc aagcatgata ggtatatatc
     5101 agattcgaat ttctgattca cgaattattt ttcaggaagc taatattttt aaatgtactt
     5161 agcgagcaga cgctagaatt gtcggcaact ttgttcttgc aatgggcttg gttctcattg
     5221 gagcagcttt aatcagctat ttgatcgaaa aaaggggtta ttagtatcgg gcctttgatg
     5281 cactctctat caccgtcttc cacttgtcct gcccatcaat tattattcca catttctcag
     5341 cgggggtttt tcctttcaat gccatatgtg gcctaaaata attatgatac atttgataac
     5401 ccgggagaac cgctgtgctt tggattttta atccgcgcat gttcttttct ctatcgcgca
     5461 cttccccatt caagcgctcc attttgttat tgttgtggtc tccttgcagt cgaatgtgtg
     5521 atatatgtcg ggttcttggg tcttttagcg tatagaattc ttttttgaaa gcatcgtgaa
     5581 aattcgccgc tccatctgtt atcatcgtgt ttggtctttt tcctgcgatt tcttttgcgt
     5641 ctttaaacaa cggttgaata tt
//
LOCUS       DAOR01000309            4911 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_112370, whole genome shotgun sequence.
ACCESSION   DAOR01000309 DAOR01000000
VERSION     DAOR01000309.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_112370"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    528..686
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1426 a          906 c          954 g         1466 t
ORIGIN      
        1 ggatcataca taagatttcg cgggaagtag tcgtcttctc ctgtggaagc ttttttctta
       61 tcatctaatg tatttacgtt gtcactcaaa tcatgttcta aagaccttag atcttctcat
      121 ttgtatcaag ttctgttacg acctttacaa tgtcgtagca tagtatgagg gtaaggcttc
      181 tacttttaga agaaaaggaa aaggaatgtt cgatatctgt cttttatgaa gtctgtaacc
      241 atttttatct tgatggggct gtcagggcgt tttttcaaga atgtcacaat agtgttactg
      301 aacaccggct atacttttct tttttccaaa taatagatag ctttcattga aatcttgatc
      361 tagaacttta cactccgttc atatactata tgatgctaat accgttacgg ctacaaaaga
      421 ggaatatcta gaaagatatg gttggtggat aagtgaattg gagtagttag ggctgagtgt
      481 aaagaaaata aacgaattca atgatttaaa cgaaatccgt agcctagnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnngaaa tccgtagcct agccgagatt ataaaacagg
      721 gcttttatca agaccataag accaccataa aacacctacg accgacgaat actttcaaaa
      781 cttttgcagg gaaatagtca agagcgacag tgcccaaggc atgcaaatta gtagttaaag
      841 atacgcttaa ccttatgctc ggttttaggc gtattcatgg ttagccgtcc actccaccat
      901 cgggaacgac atgcgcagca ggtaattgta caaggcagtc catagaatcc agtagattgt
      961 aataaaaatc caaacgatct aacatgcaca caaacattga caccaccacc aacatccgta
     1021 tgagcttagt tgtctaagag aggtttagat tgtcttcatc attttttgtg ttgctttgac
     1081 ctttcctatt cttctgatat tattccaaag tttctgcaag ccattatgga ttgtaaaaat
     1141 tggactttta aaaagacagg agctctctat ggagagtgag aggggagggg ggcggtggtc
     1201 gaatgtcggt cattaactcg actttcaaga tacaaaccac cgacagtaaa caataagcaa
     1261 aattttatcg agtcttctta cagtgttcag cagcagcctt cctcacacaa agcacgtttg
     1321 attagcccca tcatctttct gcagtctatg tattactgtt agaattcttg gttccatgag
     1381 tagtcttttg tttctttacc tctcttatca cctgctctag ataacgcttc gtttcatctt
     1441 ctgtgagaga aggctcgatt ccagtctctt caaccatcct atcttgaatt tgtttcgtga
     1501 atactagaac ccttttttct atttctcgct ccatttgggc tgatccaatg ctatctaata
     1561 atcttgattg tcctatggca aaatctcgaa ttgatttacg taagttcgag tcctccgcta
     1621 cagaaattgc tgaagaaaag atcccaacta atatcatgta cgatgataat cctaggaatg
     1681 atatcgtaac aagtccaaat ggagggtaag caacggcaat taacgtaatg gcttggtcag
     1741 aaatgaataa cattatgagg ccaaaccctg caataatcag gtactctctt acagccttat
     1801 ttgcagacac gtcttttgcc atagtccaaa aggcaacacc aaataaaatg ccgccgatcg
     1861 gtttactgag tgtgaagatt aacgtaatta ggatgctata caagatagga ttccccgaaa
     1921 gaaatagtac attcgcattt aggatgaagg caacaaattg gaacaaaaaa tacaatagag
     1981 gaagactcac gagagcccaa tacttcactt ttcctagctt cacagaataa tgccgtaaaa
     2041 gtatagctgt ggcaacccat gtcattatga aagaagcaat tgaaaaaata atgtaaaatg
     2101 aattgatgct aatcatcaga gaagctatag tagcaggata gacaggcgag aggcttatga
     2161 gacctgcccc tcctgtggtt cgattgatag attcgaacaa tgcattggta attactagtg
     2221 ttaatcccaa ttcgatagca attgtggctg atgctaaacc ataaagcagg attacaacat
     2281 ttttctttga cctgaaccaa gaaaagaagc gttgagcaag cagcagcatc atcgagatgg
     2341 ccaatatgta gctaagcatt atgttcacaa ctaggataac tacgctataa tacgatgtaa
     2401 agaccatctg caagatcgta attaaaagta ttcctgctaa cgtgtattga acaatcgaaa
     2461 ctattttgaa tagtatattg aagttaagtt tcgtttttgt tcttctagct agatttttct
     2521 gcttaagaaa tctcaaaata aaaaactgcc cgactatgaa tattattgaa attgataaat
     2581 atagatacat cttccatgcg ctaaaactcg tgctaatgag gttgccaagg ttagcaattg
     2641 atgtatctaa tattagtgcg aaaattatta tggaccacag aacaaaagtt tttttattcg
     2701 aagacggcaa ccgttgtatt tcacgatctt gcatggcgtg aacttcccac aattgattct
     2761 cccgtgtagt tattattgtt atggttcagg ggaactgagt taagacacta tcaatgaatt
     2821 gaagacctac tagaaatctg attggctggc agggcttatt tcagaatata gatggaagtc
     2881 tttataaggg atattcttaa taacacaaga taaattgaga acaatttctc aaggcaatgg
     2941 acattttcaa ttcctactta gagggatcgt gttaatcata ttgggactta tgacgttgac
     3001 aatgattcct tttgtaaggg ctgacagtat caatcctggt gtttacgcaa tagactcaaa
     3061 accctacggt atgacttatg cggattggac agtcaagtgg tggcagtggg ctttggggat
     3121 accgaaagaa attaacccaa cagcagatga tagcggaaaa aattgtgctc aaggacaaaa
     3181 tgaccagcat gtatggttct tggcagggac aggtggtgga tctgcagagc ggacctgtgt
     3241 cataccgtct ggaaaggcaa ttcttatccc tgttctcaat ggagagtgta cttacaaaga
     3301 ttcagcaggc agtaaatccc catcagatct gcgaagttgt gctttaaaat ctgatgccgg
     3361 tgtgactacc ctagcagcta ctgttgatgg tgtcaacatg cagaatgtag acaagtatcg
     3421 agtgacctcg gatctattca atgttactct agcgaacaat aatatcttag gagtagcacc
     3481 tggccctact cagggtgtat ccgatggatg gtggttcatt ttatatcctc tacaggccgg
     3541 taatcataca cttcatttta taggaacctt gggaagtcct acggtaacac ctacacttac
     3601 gagtactaat ccacgtttcg ctaccgaggt gacatatcac ctcacagtaa aatgaactga
     3661 atcacttcaa gggtctgatt acatagtctg ttggaaggac tacacgacaa gcctttgaga
     3721 attgggaata ttgagaagaa caagggatat cgcgttccat atttaacgaa tgccctgtaa
     3781 tatcacaaaa ttgcgcgtac gtgtatgatt ctgggtgtac aacaacattg ctagcgcgtc
     3841 tttcaacctt ttactattcc tggagcgaaa atcttggcac ttctctcgct tacgtctcca
     3901 attgctaata ctttttgtgt tgctattgtc gatgctgctg cttgtcgctc tttgtttaat
     3961 tgagcagctc cttatcgcaa aaaagtaaag gaaatcactt tgctaacact accgccactt
     4021 tcccttccat ccaaggatgc aatgtacagt gatacgcata tgatccatta gtgttaaacg
     4081 tatgcttcca ttgctggtgt gcagaaatga tagatgaatc aaatatccct gctctatcac
     4141 ttgtcacggt atggggcatg atgtcctcat ttatccatgt tacttcagag ttaaactttg
     4201 cagtaatttc ctttggttga aaattctctt tttgattgtt gtgccaagaa ccctttagta
     4261 ttgttactgt cgttactatt ggggctgatt catttgtatt atttaacatg ctagtgttag
     4321 tagcattatt agattcttgt cctcctgctg ctactcgtgt tttattattt tcatgtgatg
     4381 acattatact atttccactc gtactttgtt caatagaatc atttgctaga ataggcttct
     4441 ctagtctagt ttgcaatttg tattgccaag gaatcatgga tatactatgt gtcaaatttg
     4501 tattgtagct atttccagaa gctgctggaa tgacatgaat ttcacccata ttaccacgtt
     4561 cttcctcgat tccatgacta tggaacatat acgtacctgg gtacggccat tttgcttcca
     4621 caattgtagc atcacctggt gcaatagata cagtctgcgc atcataagga gtgttagaga
     4681 tcaagcctga ttggtatgct ttgaatattg tactatgaag gtggaagcta taaggtatag
     4741 ttgttccaat aattcgagag aggatatcga ttctaggata tgttcagagg gtaacttgat
     4801 tcgttacctc tcctagcaga tgataggagg ttatcatgtc catattagga cgatcaacgg
     4861 tgtctgagtt gaaatatgcc gaattaggta attgttgtac ttacacacat a
//
LOCUS       DAOR01000310            2775 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4526, whole genome shotgun sequence.
ACCESSION   DAOR01000310 DAOR01000000
VERSION     DAOR01000310.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4526"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    340..579
                     /estimated_length=240
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          928 a          429 c          474 g          704 t
ORIGIN      
        1 gcttatatat tgtccccgaa tttgaaggac ttgggcgctg aatgcaaaga gacgggagct
       61 gccaagacta agtgtttaac ttgattaaaa aaataacttc taaaattctt ctaaaaatca
      121 cgatcttggt tatcgttgaa attactctga ttgttggtag tttcagtgtg cttgcttatt
      181 ttcagtccca gcaatcatca atagggaatt caattaatat tgcagggaaa aaccgatatc
      241 taactgccaa tcttctattt caaactgaaa aatatataga cgggtcatct gatgcatcac
      301 aactaaaagc tgctatggat agtttgcaat caaatataan nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggatagtttg caatcaaata
      601 taatgacgtt aaaacagggt gggagaattt caggaattga tttaagacca ctgccttcag
      661 gcttgttaga catgtggaaa gctattgacc aaaactggaa tgattacaag acttttattg
      721 cacaggaaat attgatacca caaagtgcaa aagaaacgac atcgtcaccg ctgatagcaa
      781 tagaccggtc gttagcagac aaaggcctcg aatcgaaagc atataacttg gttaactctt
      841 ctgataagtt ggttacacaa ctagggcaac aagcagataa aaattccgag aacgtaatgt
      901 tactgcaaat ccttttcgca atcctgatag tcggtattct agtattaatc ttgtatctcg
      961 ttgcaagaat gctaaaacca atattcgttc taactcgagc gacatctgaa ataaaaaagg
     1021 gaaaccttga cgtatcaata gcacataaag gtagtgacga actctctgtt cttagtgagt
     1081 catttaattc catggtgggt tctataagag actctaccat aaaacaagac gaattaacaa
     1141 agaaacttga agtagcaaac gaggaactca aatacaaaga tcgattaaaa gatgaattca
     1201 tcaatatagc agctcatgaa ttaaaaacac caatacagcc gatactcgga ttaacagaaa
     1261 ttctttactc tcaaataaag gatgtcaaac aaaaggaatt attggaagtt actattagga
     1321 acgcaaaaag attacaacag cttacgaatg atattttgga tgttgcaaaa attcaaagta
     1381 aatcacttgg attgaataaa gaacaattca atttaaatga tgtagtgata aatacaatga
     1441 atgatctggt attaggcaaa gagttccatg acactaacag ccaaaacata aagctatcat
     1501 atgatcctca agatatattg gtacgagcag acaaaggaag aatagctcag gttatttcaa
     1561 atctactgag taatgccttc aaatctatca aagaacacgg gaaaggtggg actgtaacta
     1621 tagatataaa gaacagagtt agggcaggag gaccaaataa aaaagacctt gaaatcgttg
     1681 tcagtataag cgatgaaggc aacggtatag acggtaacat attgccaaat ttgttttcca
     1741 agtttgtttc aacatattct tcaaaaggaa caggtttagg attgtttatc tctaaaagta
     1801 ttgtagaagc tcatcgtggg cagatatggt gtagaaataa tgaccatggt aagggagcaa
     1861 catttggctt tagtctaccg atatgaatac tactattcgc ctaagtattt tatacgaata
     1921 cctatgcaaa gtgaaaggct cgaagcgtgt agacgagata atggctgaca ttaagatgat
     1981 gatgatgctt aagtaccacc tgaaggtttt ggaggtgtgg agaagatgcc ttcgatgcta
     2041 gataaagagc gcagttcgag aactaatatt gtcatattag ctgttattgc aggtattgca
     2101 ttagtactat cgatattctc atatgaatat tcaattctta cttctaacaa aatagttgat
     2161 attgcttcac aggaagtgag atcaaatacc agaatcgaag tgcatgacat ttcactaata
     2221 ttagcaaata aattacaaac tgtcggtgct cttttacaaa cacttgctga gtctcctgcc
     2281 atacataata atgaatacca aagagcacac attgttataa acagtaggca acactcctca
     2341 agtgatctga cagattttta tatgtggtta gataaaaacg gcaaaataaa ttggataagc
     2401 aatatcaacg aaactacata tcaaaaatac aaaggaacag acctaagtta tagaccatac
     2461 ttttctatcc caatagctac tcatacagta tattacagca gtctgataga atcaaacgac
     2521 aaaattccta gattatacat atcataccct gtaatcaata caacagggac aaggacaggt
     2581 accgcgaatg gaattgttac tggtgtagtg gttacctcaa tcaggctcca aaccctaggc
     2641 aactttttga gtaaccaatt atttccacag tttaatagta ctataggatt actggacaga
     2701 aatggaacaa ttctttatgc tacaaatgcc cagcaacatg ttggcgaaaa cgtttttgga
     2761 gataaatttc aatct
//
LOCUS       DAOR01000311            4686 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_85030, whole genome shotgun sequence.
ACCESSION   DAOR01000311 DAOR01000000
VERSION     DAOR01000311.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_85030"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2474..2656
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1234 a          909 c          858 g         1502 t
ORIGIN      
        1 tccggtgtat ggggctgtaa ctccttactt tgtatattca acaatccatt tcgtaagcta
       61 aggaggtcaa tattagagtc gaattttgag cgatctacat aggtcctacg tttaatatga
      121 ccaactactt cgttgagttc atgcgttttt atttgtggac acatcaactc gcagagttca
      181 tttattaatg attctccacc caatacgtaa agcccttttc cattatagta gcagacatct
      241 tcattatctc tcatagtcgc aaaaaaatat tcattcatga tatttcgagt tagtcgctga
      301 ataacatcac ctttgccaat tattcgaaat atcttgtcta gaatttcctt tgcaatatta
      361 tgatctccag ttacacgttc cagagtttct aaaagatact tgcttcctcc tacaatcttc
      421 tgatcttgtt ggtacgtttc ttctatcgtg gcgacagctt ttcttctgtc ttgttctgag
      481 tcctcttttg ttagtatttc tataagggcg atggtagaat cctttgacac attggattta
      541 tggaataatc cagatagggc aaacactaag tggtgccggc gtggcttttt ataataggca
      601 tatatcgcac tcgatatcag ctcaatctct ttatcattta gttcagtctg ctgtgcttta
      661 cttgtattat cataggattt ctttcgttcc ttgctcttag tctctaggca ctcgcttaat
      721 gtttctatta gcttatcgta tagttcatct gagatgaata atttatcaat tccaaaattc
      781 gtatagcgaa attcagggtc acggtctctg tgtctactag gcggaagcgt tgaaagactc
      841 ttatcagctt tgatctcgaa ctcgtagcct tgtttacagt ccgccgtaag aattggttta
      901 tgttgataac ttaaccagta taaatggaaa ccgtatggtt ttctggtctt agttacgaca
      961 gtacattgtt gcatttttgg tattagagaa tactcttcac tatctccatt ttgtaaccta
     1021 aacaatatct tgtaaacttc ttcgctgtcg atatctattg catagagata caactgtccc
     1081 tgatcgtctt tcaaatgtgt tttgccgaat gccgtggcaa cgttcttgaa cctgtatgac
     1141 tcttgtacta atttgtctgg attccaatag ttcacgttat cataaatatc tgtccaagct
     1201 atggccggtt tgccgtcctc atcaagtggc accaacttga aaccgatctt atggaggccg
     1261 attaactggg ctggacgtat gacttcagca gatgtcattt tcggcttggt gctttctcag
     1321 acagtttcga cagtaggaat ggcggtacta gtactactag tgatgatatt cgccctcgct
     1381 ttttcaaatg tgtgttgatc tatctctata ccgatgaatt gccttccccg aagttgtggt
     1441 ggtggtagcg gtgacgtact gccattattt atcaggtcaa tacgtttacc ctggaaattg
     1501 ggctcgattg actctgtatt tctttatagc aagtacttcc tcaacagtta aattctttct
     1561 gactaggtta gcatcaatct cgccatcttc tctaattgga acgtcaacaa catggacggg
     1621 gatatctgga atgcataatt tcctgtatgc tgcgatcctc cgagtcccgt ctatcaggag
     1681 gtactctcct tttgttacag ctatgggaca aagcagacca ttatttctta tactttctac
     1741 tagatcgtct atattcccta agtcttttcg aaaccgcttg ccaatctgta tgtcggagat
     1801 ttttagcagc tgattcgcta ttactgatgg tgattgctgc tcaatctgtt gattttgaac
     1861 agaagtgtga gattttttcc ttcgattagg tctgcgctta gtaatgtcta ttaatgcaga
     1921 atgcacatta ttcgtcgtcg tattccatca gctcctgctg gtgttttttc gaccactgca
     1981 atctgacttt ggccaccaac ctgggcagat tgcggtgatt tggttatttt gataccgttg
     2041 ttgtgtactt ttctcatttt ttgtcgcctt atgccttttc tataatgatt ctgttttcct
     2101 cttggtggac cttgacaaaa tcgcctttgc ctattcctaa gtggatagcg taggatttgg
     2161 gaagaactac tgaaaacgac tgttctccga caagcccttg aactttccta tattccatat
     2221 tatactcaac cgtgcctttt catgaagtat atataatatg gatagcgaaa aagggccatt
     2281 gatttttcgc tatcttaagc aatgctcgca agggctgttg gacttgtgta atatgtctga
     2341 atatgtttcc tcgttgaggt caatggatcc cttgttattg gtttattttg cgagtcatcc
     2401 tgcgtcctca gcctatgaat ttgcagatcc atccctagtc cgatcggcta gatcatctaa
     2461 cagttccaaa gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnncaca tggcttcaca ggcatactgc gagtctgtat cgagacgcac
     2701 aaaaccagac taggagactt acaaaattaa aattaatcga ggaggtagcg gttcaaacta
     2761 gcttgcataa agctaaacat tacaagctaa ccaaatatgg agtatactgc atcatagaga
     2821 aagtgccttt gacctctgtc gttgttaaga gtctcctaag atattacaag gatcatccac
     2881 tgtttcaata tttcatctat ccttggataa tgaatgatac tcttttgagt ataagcggca
     2941 actctacatc cttcttctcg caattatcct cttacttccg ctactgtctt gaagcagtca
     3001 aagatacgat tgacgttctt gatctgacac aagacaactt catatgggaa gatattcgga
     3061 ctggaagcaa agatgcaaaa gcgctatgta attttttggt acaacaattt ggatggaaat
     3121 ggctgggtaa cgcctacatt gagaaaagta atagcaatac catagagatt aggggagccg
     3181 gatccaattc cgttttaatt cgtcttagcg atggtaaggc cattgtgagc tatagaggca
     3241 aaaaacaagc tgaactaccg gtgagtgagt tttttcataa attgatagtt aagcagccga
     3301 ggcaattgga caagaccttc atgaagagct ttgcgaaaac tcatcgtgga gttgttcaaa
     3361 agcttctagt ttctataatt tcggatgaat ctatcgaatc cgatacaaca agaatcctgg
     3421 cacaagatga gaccttcctg gaggcaatac aagatactag gagccaattt gaacataggt
     3481 acaacgtttt cttgaaaact aattaagtgg tatcacctgc tatccgatta tacaacacta
     3541 aaagtatagg gattttgtat tggcatttga tgtgtagaca gcaggatata atttcagttt
     3601 tttataaatc tttgaaaatt attaggattt cttctcttag tatctcacta gatcacatat
     3661 cttctactat gcattgtttg actatttcga gtcttgtatt acttgagcca ttgatctggg
     3721 taaattggga tatcaaagtt ttagtaacta tcgcgatatc aatttaacaa catataccga
     3781 atatttgtat aattgccttc agtcctatta tctacccagc tgttttgtag cttttgtttg
     3841 aataatggtc tttagttata aaggtacctt agtgtcaaaa tttctctagg aactcagtgc
     3901 ttcataatct tcatatcagc tttaacatgc tgccaaccca gtatttgtag cgtttaatgc
     3961 taatcaaatt ctcttattta gcaagccatt ttgtaaattt aatatgtagg gttctgcatc
     4021 gaacgttaac cggtctatcc cagttctacg cttaatgtga ttgattactt cctgcagttc
     4081 atgcgttgtg acatggattg cagacaatcc caatttcgtt cccaaattcc ttgcaattat
     4141 ccacctacct agcttccttg tatttcttac cgttgtaacg gtataatgtg gcttggctaa
     4201 tcattactgc ttcacaaatc tccctatcgg tcattctttg cttcaaccgc atcatactaa
     4261 tttgagccat caagaataca ttgtataaat catttataat gtctaagtgc atataatgga
     4321 tatgactaaa gagaatagtc gcccatacaa aacgagaaat gtttatccaa ataaaagagt
     4381 tccggctcaa atgaaccctg aacaaattgc acagtcaata aagaacattg ccaaaggaat
     4441 tcacgaagga tcatatcaaa tgagaaaaac agtacgaatt cttcatcaaa gtggagcatt
     4501 taacgagcta acacaggcgg tatattatgc cacagttgct gcgcgtgaca ctgcaagaga
     4561 aattcgtgat actgctaggg atttgaaaga ggacggtata gtccgcggta cagctagcgc
     4621 tatacaacaa acaactgtgg cagcgcgtgt gacggtacac acagtcagag acatggcaac
     4681 agatac
//
LOCUS       DAOR01000312            3336 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_21965, whole genome shotgun sequence.
ACCESSION   DAOR01000312 DAOR01000000
VERSION     DAOR01000312.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_21965"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          950 a          632 c          590 g         1164 t
ORIGIN      
        1 taacataaaa aaactgggca catatgggtg cttaacgctc aatgttatgt tgacagattc
       61 ctttacctcc atactattct tgttcatcag taccgcagag cagtacgcaa tgttgttcct
      121 gtacgcaacg tctacaccgc aaatatagat tatttcttta ttgggaatat tctccgatat
      181 taccttcttt gctatctcgc tttgcacatt tatagtggcg gccaaatagt gggaagtcgt
      241 atttcctgac actgtggagt tgctcatgct ttcttattat acggcagctt tccgcacgca
      301 tatatcgacc tatgtattcg accatgattt gttttgtgat tggtaattta gtgttcgcat
      361 gtttccactg acttggtagt gtcaatatta ctggtatttg actaactttc tctacccatg
      421 gcttcaattc ttgttctgga tacagataat gctactaata ctgtaagtcg gacaatccta
      481 attcaaacaa attctccgct tttttattga tttgagaatt aaaatatttt gaggatagta
      541 aatagaatag cctttccagc ggggatgtta cggggtcttg ggatctacta cgaactggtt
      601 gtaccttcga ttaacatagt cttgaatgat tgccttttgc ttcggtttga ctctgaatcc
      661 tattgttatc agatcgtctt gtttttcatt agcctcacta atagataatt tcgataattg
      721 gatcaaaacg tattaagctt aatacaccat tttttaccta ccctggtcgt ttggataggg
      781 taaatttagg atcgtagaac aatgttcaat aacatctaaa atgctttatc gtatgctcat
      841 acgtaacatt actttttaaa atttattgta aaaaagtacg gaaattaatg ttgtaccctc
      901 ccgggagggt acggcctatc atgtgtacaa agacaaacta tatgaaattg gctgctgcaa
      961 gtggaaataa ctttgaagac tgatgttaat gatgctgaac agcggctatt tgatggaaac
     1021 tagcgtagca acctacagga tatcgaatta ttctctgcca aggaatttga attggtagtt
     1081 gataggcagg ttatcggtca ttgttgctat tcacaacaaa aggaaaggta tgtctattca
     1141 gtttccttcc acagtaggta acactcaaag tatttgaata caggttagcc aagttaacta
     1201 aggtgcaagc agaaacattc gtaataacgt tcctaaaatc tcaagtgaca aaactcatgt
     1261 aaaatcatat agtctatcat accgtgtaga cactttatta ggtttagacg gagatttttc
     1321 acgttatctc ttgttgaact tccctacttt tttagaccac tttatagcaa ttttctgtgt
     1381 agctacatgt aactctttag aataatgacg aactctttac atatgttcaa ctttaccgat
     1441 taagagttgt cgtgtaacga ttggtgccaa atccaattca agtcttttta tctgcctgta
     1501 tctgtttctc aaggtaactt cggttactcc tgctgcctga gcaatatcag tttgagttct
     1561 tttttcacca gtatatagac atgctatgta cagtactgtt gctgcaattc ccatcggatc
     1621 ttttccagca ggtatttctt ttttattaag atgatacata atatctatag ccttacgttt
     1681 ggtcttttca ttcaaattag ctgtgtttga aattttaata atacatttcg tagaatcgat
     1741 gattggaatt tttatatcaa gttcagtgat tagcagtcta taactttttg ctaatgttct
     1801 acgtttaata ttatttgcta cagcgatctc ttttagtgtt tttggaattc ctaattctct
     1861 acacgcaata tacaaagcag catccaatac tgcagatatc gcccttccac gtacgagtcc
     1921 tctttgctga gcttttctgt agatataagc agttttctca actattgaat caggtagccc
     1981 aagtttgtct tttgatgtgt gaagctgatc gaaggcatcc ctcagatttc gattattaga
     2041 agtactagtc tttattctaa ggtcccatct tcttagcctt tccatccttg aacgcatagc
     2101 tgcgtcgagt atgtggccac cagcatcttg attcgacttt cccataatag tagaaagacc
     2161 catatcataa cgagctaaag aatttgtaac tccagttttc ctgctcttat catacattgc
     2221 atcagttgaa tcttgaatat tatcagaaat cactagacca cagttactac aaatgacctc
     2281 tccagattca tgatctgtta ttaccctgtc actcttaata caaaacatat gatgttacgt
     2341 cgctccatca gaataccatc acatgcactc tatcttctac actttatttc taatgcagat
     2401 agtcgtaatg ctatccaagc ttgatacatg tctgctcgtt ttacccctat tagtggtgag
     2461 aatacaactc tactatttca ctcttgtaat acattcgtcg aacataccaa acaattttta
     2521 tattaatcaa taagataatt cgatgatctt gaaacaccaa ttggtgaaaa ggcatcacca
     2581 cattggattc atgtttcatt tgctagtatt cttgatgctg ttatcgttgt tatctattct
     2641 atcgtcaaat gctcaaagct ctgaggcccc ttagcagact gattgcaatt atcattcaga
     2701 tccctgcctg cctgatcgtt ttatgaacat ctgttctgga aaattcttgt tattggattg
     2761 cgaggacatt aagaatttgt tttcgaaata ctccgtagct atctgttctg ttttttgtag
     2821 ttgtctagca gagtaactcg gaggtatgtt tatttggata gacaaaaggg ccttcgtgta
     2881 gctgctaaat aagagaggtt gaaacattgc aaagagaaca attgcctatt gcatgtgcaa
     2941 gatgtaagag cccttattgg cacaagtctg aaagaaataa atgttgaagt catattttca
     3001 tcaatgtctc aacagacagt aaggtatcat ctgttgctgg aacaatggct tgtatgattt
     3061 ggaaatggac tccatattat gtgcatattg aatataacgt agcatctttg atggttatat
     3121 tgctagggaa gaagttattc agagcagtaa tcgagtacgt tgacatgtgc tcagattcta
     3181 acggccattc catgtttgct gtcgttaatg gtccagtagg taaagtatgt cagaaaatcc
     3241 aataccttca accgtcatac gaaatcgtat agttatttat attagcgcat ccagggctaa
     3301 atcctagata tttcagatta taaacttttt aatcca
//
LOCUS       DAOR01000313            4306 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_73315, whole genome shotgun sequence.
ACCESSION   DAOR01000313 DAOR01000000
VERSION     DAOR01000313.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_73315"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2579..2775
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1117 a          860 c          795 g         1337 t
ORIGIN      
        1 ggggaagtaa atgccatcat attcccacca aacggatttt gctgcttttc ggagcccggc
       61 ttgaaatcat ttgggtcatt taatgcgatg taaacgtaag gcgcgccatc ttgagaggct
      121 tcaattcttg aaataacata atccttcttc atcgcgttac actgtgaatg aatagactta
      181 taatttttcc agaacgcccg tttctgattt tagatatgtc tcgcctgacg cagcaggatc
      241 aaatcaccct ttattatggc ccgaaatttc attcacattt tcttgctgaa tacaagataa
      301 agagtgactg gttcttcata ttcttctcca atccagagta tttcaaagcc gttcattaaa
      361 tcgtggattt ccttctctgt aaagaatcgg acttgaaatc cgtttatatc atagattcct
      421 ttatctattt ggactccctc gccataggat ttgtcatttt ggtttctaac agaaaagaaa
      481 ttgaacccct ttggtttgag tactctcctc atttctgaaa atataaaatg aagctcgtcg
      541 agtgaaaatc gcatgttgag aagcatgtgc gaatatacgg catcaaaata gccattcgga
      601 aatggaagcg cgctcttgac atcaaagact cgtggtttta tcggttgtct tttttccttt
      661 gctatcctat ctagtatctc aacagcaata acagaaaagt ctaatgcttc tacttcaatt
      721 ccatttgatg caaaaaacat tgtatctctt ccatgacccg cacctagttc caggattctc
      781 cttacattat tcattttcat gtgattaaaa caaagcagag caaagttgct ggcttcctgt
      841 ccaaagaaag tgttatctga tttgtagact ctattccaga cttcactcat tatgtctccc
      901 ttttgtccgt ctttccaagc agatgaatac ttagccatat tcgtgtaata atcttgccag
      961 tcccccctct ccctcctctg ttggtcattc atagtatata gaagaagcgc ccccttcatc
     1021 gtacaatgtc aaatagacta gtgccacaat tagaaaagtc gaaggaagag cccgtcaccc
     1081 aagccaaagg acaagattta tctattgaac aggaaattga atgtcctcgt tgtcatgagg
     1141 ttatgacatt gtgttctcaa tttgatagac taggctattt ttgtgaggaa tgtagctttt
     1201 cgctgcattt gagccactaa aaacctgcta aacaacatct caggcccaag taaacgacgt
     1261 tccttgagcc gttgtcgttg tcttggtggt gcatatatat tcacaattaa ttatttatga
     1321 attacagcct ctttgtctgt tgtcttgaaa tgatttgtgc gcccgtaata tagaacactt
     1381 ttataaaata cggcacaatc catacccaag ctgttataaa gtacgaaaaa taaaatcaga
     1441 agagatttga caaaatccaa aattattcct tgattgggcg ctccatctcc ttgtaatatc
     1501 actggtcctt gtaattttgt ttgaattaat tcggcgcagc agacgtatac tttattttat
     1561 cataatttaa ttgataatag aaaaatgctc actggtgttg tcattaatat tgacccctta
     1621 taatttcaag aatttctgaa cactgcgtaa agaaattgcc aaccatattt attctagtat
     1681 tttatattta tgattttggt gtagacttgg cttaagagag aatttctgct agggttgtgt
     1741 ggttcataat ccggccattc cgaatctctc tttctatgtg ggccgtctac accgttttag
     1801 atttatccgg tatggtgtgg tgcttcatga aatgttgcat gtctatctta gattactatt
     1861 gtcttgttgt gatgtcggcg ctctttttag actctgaaag tagactacca catttcctca
     1921 gacgatcgca aaaaatcaat agacttgtct gtctattgat gcagaaatat tgcagaaccg
     1981 gacgtccatg agtcattcag aatattactg tatagtacgt aatgaaaggt acgtaataaa
     2041 ggaagcatga taccgtaatc aattcggtcc atagaatatg ttcggacgca gccctctccc
     2101 tcctccattg gttattcact ctattagaag atgcgattgt tgacaagatc atataatgtc
     2161 aagcagcgac aaattagtac taacagataa aacgaaggcc aaggaatcta tcacaaggtc
     2221 aaaggaaagc acggataatc tatcaatcac ccatgagatt gaatgtcctc ggtgtcatga
     2281 cactatgatc ctatgctctg aatttaatag actctgttac ttctgcgagg aatgcggttt
     2341 ttgtcttgct tggagcactt gacagtgtag ccataatcct tgttcattta gaaatatggt
     2401 gagacagtaa atgttcagag agaaggcgtt cgagctcaac ctgaccagca tttgttaccg
     2461 tatatttatt gaaggtgaat gtctttgcac atatccttag ctgaacggaa tggaatatct
     2521 ttatatcgct tcatctttct ttttcctttt tttattcaca ttagatgata tgatttccnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnntgaat gctatattgg ttcagcgcga ttgaagacca ttggagacaa
     2821 agaaccaggt ttggaagtcg atgactgctc ttagtatttg aagtcaagtg aacgaataga
     2881 tccaggccag cataattatt gactacatgg tgtaatgatt cgggaaatgc taccgtgaat
     2941 cattacaccg gccaaaacca aaaaatgatg atcggtactt atggaccaaa cattacaagc
     3001 tctgttcagt tggggccaag ccccttaaat aaatcactaa caaaacagca gtaatgactg
     3061 accatatccc tgtccgacgg ctcttcgaaa cacatcttac agtgagcgat cttccgaggt
     3121 ccataagctt ctaccgaaat gtcgtaggcc ttgagctggc gtttgagcca tccgaacgaa
     3181 atgttgcctt cttatggatc ggcgaatctg ggaaatccat gcttgggctt tggtcactcg
     3241 gcacggcccc gctaggcctc actctgcact tggcgtttga tgtggcatta aacgatctcg
     3301 tggatgctcc aaagcgcctg aaagagaagg gaattaaacc actctcattt ttcggcgtag
     3361 agacaacgga gccgagcgtg atttgctgga tgccggcagc ctcggtctac ttccgagatc
     3421 ccgacggtca cacgctggag tacttgacta tgctggacaa agagcctaga ccggatttag
     3481 ggatcattcc ttggtccgag tggctagatc agagcaagtc ataggctgaa ccattgatga
     3541 ttgtagacca cgaatgcgct acattatcca agaccacatt actgcatgac cgttttatct
     3601 ttggattgtt gccgcagatt cagaccgcgt cctattcaaa tattcataca catttagtac
     3661 ttcttgatct gatatgccat agccttgtag ctttagtaaa gatgttatcg ctttgtttct
     3721 tggtgcgcaa atttcgttca ttccatatat ttggacggca agtctggata tttctttttt
     3781 tagcccgccg attctaccat aattctcgat gtcgtcgatc acacgataag ctgcagtcga
     3841 aatcggcaaa ttatttgtct gggctttttc gtttaccaga agactaaatg gtaataattg
     3901 atctatccca attcccattg accgaatccc ttgtaacagt ggtaggactt gtctatctgc
     3961 tgccattcgt ttttcgagca tttcgcaatt atttttgatc tgcctctctt tttgcctcag
     4021 tgatttcata ctagcgaacg cggctacaat tttctggggt tcgtatccta tctccttgat
     4081 cgttagtaga attgacaaaa gtctagtagg atcttctaaa gacagattgt gtttactcaa
     4141 acgttcttct aattgtttat attctcttat cgtttctata tccacatttt tactttgcag
     4201 aatcgcgctt gcttcttgta tttgttcttc gagtctttgt ttttcttcct ccttatgttt
     4261 gatatggttc ctcaggtctt cgagtggtat tgattcagat cttgat
//
LOCUS       DAOR01000314           17925 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_45049, whole genome shotgun sequence.
ACCESSION   DAOR01000314 DAOR01000000
VERSION     DAOR01000314.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 17925)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17925)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17925
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_45049"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         5491 a         3402 c         3298 g         5734 t
ORIGIN      
        1 gtcgtccaca tcataccaag ccaccggagc gccacatgaa tgacatcgta agggcttaac
       61 tttcattact tatttttcac ccttaaagta ataagcttgc gctactgcat tataaccttc
      121 ttcatagcaa tatttctcct ttttgtatac ttcgcatgtc atttctggat atatcgtcct
      181 gtaataggat ctcgtttcct tttgaccagc ctaacttttg ctgattcact cattttcatt
      241 cttgattcta gtgaccaggg tttgtctttg ttccaaggta tcttactttt acgagctgca
      301 ctcattgtct ttaatacctg atctgaaaac acgccagttt tccccttatt ccaaactctc
      361 ctaccagaac aatatccttt ctttatcact gcactcggtt tctttattgt ctttagtgac
      421 ttaggcttac catacatatg gtgattttta ccagaatgat aaattgactt atgacacttg
      481 cggcagagat acatccaatt actaaaatct ctgttatatg tacctgtaac atttgctagg
      541 tcataatacg gtacttcatc gcatatctca catttaccat ccaaaggttt tggcttattc
      601 ttgcggagcc agcgatgaag gttctctaca ccaacgtcat cacctctcca tccattacca
      661 cgtctgccaa acatgggatt attcttacct gaattcttac agcttcgact acaatacttc
      721 tgattgtggg ttaattcttt tccacatgct atacacgtca taggtttcaa aacctgcgaa
      781 gtgatgttac tcaattcctt ttaggcccta ccaatgcggg ctaaatcatg taagtggtag
      841 ttttggaatt cggttgtccg ttcctaacaa cttgcatcaa gcttacgcct tgtcccatat
      901 agtatattat ctgacgatat tcccttaggc cacgttccca gatacttagt tcagtattgt
      961 tagagtcatt ggttagatca tagcattcga attgaaactt ggtttgagtt ttgcctggta
     1021 tcagttccta ggttacaaag tccttcattt gtgattgtac ttctttccat ttgtcagtag
     1081 tgattgtact tcttttccat atggtgtgcg aactttatgt aatttacgcc tgcaactgga
     1141 tgctagctaa agacagggac gaattggata agttaggcag tagacatgag agttacgata
     1201 tcattattag cagactcatt gaagagctta aacaggatag gagtcaaaag aagaaatgac
     1261 taaagacagt caaataggag cgcttaaagc gctaatcaaa gaagctaaag aaacattgga
     1321 attagcacat aaggagttga ataagaattg acaaacaaat cagcaaagaa gaagagcaaa
     1381 aaggcaggta aatccacgaa atgactaagc caccgccaga acacttctta acaaaagaaa
     1441 aattcatgaa gttacgtatg gatatattta tggtgtgtgt gtaaaaacat ctacatccgc
     1501 tgatggaagg tagtttctaa taccttttaa atagcttctt tatcctcatc aactgtatat
     1561 tctttatatc ttgtgaaacg tcatcgtcta cattttcatg tttattggcc tttgtgtttc
     1621 agtgtttcag tggttttttg ttataaaaat taatgcaaat taaaaacgct tttaatcagt
     1681 catgagattc aagttattat acattgaata agttgaaggg gaaaataatc gtacatagat
     1741 acgagagcaa aattctcaaa tctaatccgt taacggatcc tcatgtaagg gatgttataa
     1801 tttaccttcc tcctgaatat agccaaagtt cttctcacgg atacatcgcg gtcttcggac
     1861 ttgctggatt tggaggtcaa ggaaaaatgt tgttaaatgc taacccatta ggagaaaata
     1921 tcgaagacag gatgaaccgg ttgatttctg agaggaaatg cggtcctatg atcttggtct
     1981 tacttgattg ctttacaagg tttggaggaa atcagtatat caattccaca gcaacaggta
     2041 gatatgaaga ctatgtcata aaggaaatcg taccatttgt tgataagaac tacaacattt
     2101 ctgcacgtgc agtttggggg aagtcttctg ggggttatgg ttcaattgtt ttgggtatgc
     2161 gacatccgga agtgtttcaa gccctagcgg accactcagg agatgcagcc tttgaatatt
     2221 gctaccttca agattttcca aaagctcttg atgcgtttag agaagcagga ggatctagaa
     2281 agtggttgga taatttctgg aaaaaaccaa atcactttca gcaaattgac gagccccctt
     2341 tgaatgtatt agcaatggca gcacactatt caccaaatcc aagatctgat tttccagtat
     2401 ttttggtatt tgtattgact cccacaacat ggacaaggca tcatactctg ctttgacgca
     2461 aaattttaaa aataaacgct ctctatgagc tttcgaaata ggctagtact aaggttatat
     2521 cgagaacctc attcagagat caactcggac tcttaaaatc caatgcaaaa ttcgtactat
     2581 gacggattcc ataatcaaca atatcgctag tgattttacg cacgaaatta tataaagata
     2641 ggccgtcagg gtaaccatag tgccaatttt aatcctaatt ttttaatcag gtatattggg
     2701 ttattctgta tcttcatttt tattattcag aaatcacaga ttgaaaatgt actatggcta
     2761 cttaaggcag ttttttctca aggcacgtaa ggcagaatat tataatataa taatgacgct
     2821 ctaattttat gctattaagt ttcgtagcga tttttaattc ccttaagatg ttcctcaatt
     2881 cttgctttca attttctaat cgaaatgctg gattcgtata aatgggttcg tttctaattg
     2941 ccattatcgt agtttagact tgtattacga tggtcatcct ctgcgtctgg cgtattcttt
     3001 cctacaagtg agacaaatac atcatctagc gttataggtg aaactgtaaa cgatagattc
     3061 tttttgatcg caaagtccga tatttctttg acggtagatt caaaagtaaa tacccgtaaa
     3121 gtacccgtcc cagtgtctac aacagaacca taagatttaa gcagttcaat atccatgcta
     3181 tcttttgcaa tatcgattct gatgttttgt ggtataatct ttcgtaagtc ccgcatagtc
     3241 ccatccgcta ttactcgacc tttgtctatg atgactatac tgtctgaaag catctcagct
     3301 tcatccatat aatgtgttgt taataatatc gacttgccct catttttcca atcctttatt
     3361 gctgaccaga cttgacgtcg tgaaactggg tccaatccga ttgtgggctc gtcaagaaaa
     3421 agtaattgag catcggtagc catagccata gccacgagaa ttttttgctt catccctccc
     3481 gataagttca tcgcaggtgt atcttttgct tcgtaaagtt ccaacttata caaaattttt
     3541 tccgtcttct ttttagcatc gtatctgctt tcgcctctga tttgtagcca attataaaca
     3601 tgatcccatg gtgtaagtgc tcttaatggc cggctttctt gcggtaccac agaaatcaga
     3661 ttccttatct ttcctgcctc ctttagtatg tcataaccga ggacacgtac gattcctcta
     3721 gtaggggcca actgggtagc acagattctt acaaaagtgg tcttccctgc accattcctg
     3781 ccaagaagcg taaatatttg gccaggttcg acagccaccg agacgttatt caaggcagca
     3841 tagtttgaac ccttatacaa tttatacacc tgtactgcct cgactgctag catattgagt
     3901 atgtatctac attaacaaat atatattaac taggacaccg aggggattct gggctattca
     3961 acggataatt ggtaaattat aacgtaacag acaaattgaa ataactcgac catgacaaat
     4021 attcatctgc aagttgtgtt atttttcatt ttctgagatc gaggctttat tatctttata
     4081 agcctttact aaagtgattt taaatgtaat aatacgatat caaaatggat gtcttcgagt
     4141 ctcggagtat ctatgatact attgtgccct attctgctac ggtcagttct gttgatctgt
     4201 cgtcctattc attcattaca ggaatctcaa gaagctttca gtacaacaat taacagatta
     4261 aaccaatagt ggttgtcgaa tgtttttaac tattctggaa cacggatgtg ggctgctttc
     4321 ttccttctgt ataggacata ggttaaacct actagtatta tagaaagcat tatactactc
     4381 aaggtcaacg tataatttcc tccattggga attattaaac acgggtaatc acgatagacg
     4441 cagcctagcg taaaaaaaat cccattccat ccagaatggg taactattgc aaaccagcct
     4501 tttccactaa tccaggttct aaaactaaag aaaaatgaag gtattacaaa caaccagttt
     4561 gcatatgcaa gattgcgaat atcaagggca tgtgtgttta taatatgtag cattgcccag
     4621 ataatccctc ccgctagaac cacataatga tttccaaatg cataataggg aatgccaaaa
     4681 aacaacgatt cttccgttgg tccagcagac aacactagag caaggtaatg aggaagtgaa
     4741 ggctcttcat aatttgaaat aaccttttgt actatagata ttcctatcaa cataagcagc
     4801 aggcctatcc catggtagaa catgagcatt atcgtcaaat ggccaatcga tgttcgccgg
     4861 tatctggaca accaagatct cggattcata tcagtcttga tatcacagaa ttttctctct
     4921 gttggtggca gtgtcaatga ccttaatttt ttctatgcaa tgggatataa aagtacaaca
     4981 tcctttttat ccagagaagg aatcgtcagg ctagagtggc gatattcatg ttatcctgtt
     5041 gagagtagtg aaattccact gaagttatag attcggatct actacaacat atgctgaatc
     5101 agaaattgga gaatgaagga tcaaaaactt cgcattcttt aaacactaat tttctgctgc
     5161 ccgggaggca gaccgataac ctctcaacaa ccattcgcaa atttcttggc agtgaataat
     5221 tcggtatcct gtagctgaga acgttgtatc gcgaatacca aaatggtaaa tagaatattt
     5281 aacagataat agaatggtct gttatctgtc tagtctgatt ccaacccgtt atcttgtcag
     5341 agcaaaaatt tgtaggctca ctttttttac tccacaaacg cttgcagcgc acatagattc
     5401 agaacatcca gaggtagaac aatcgaagat actgtagtat agtaaaggat cacgacaaat
     5461 ataccgcaat cctttcacag gtcctattaa cgggacaaat tctgggtcat tattactata
     5521 catcatttag gagtctctta atccctcttc gatggtagtg ttaaatacaa tgcccttttt
     5581 tggtttgaag aagaagatgt tgtgatagaa aataactctg ctaacaaagg atggtgcctc
     5641 gaagaaatga gactaagaat cgtccaaatc ctaaaggaat ttacaggata atgccgccac
     5701 atctcaaaca actgatctga gtagttctaa gattaaagga tagtattact agctacaacg
     5761 actaacgacg atgttaacaa caatgcaatt gctgagactg cagcactact attatttagt
     5821 ttcagactct atacatttcg tcatttaggg agatggacct cttatcaagg tggcagcatt
     5881 agtcgtattt ccattggcag atgatggaac actctttatg taaaatacgt tatttgttgg
     5941 atcaaatccg aattgattta ataacaacac cttaaatcca ttatcgtgta ggtatttcat
     6001 ttcttgggca aataaatcca cggttgtgct agaagcgtgg ctgactgggg tataatcttg
     6061 aatgctagtt gttagattgt gatacacgat tataggaatt gcatttattt ttccgtttga
     6121 attagaagga atttgactat taataatctg aaggaagact tgaaacattt ctggaggact
     6181 aaaattgtgt ttgttactga tatggtaaaa gctagcactt ctaatatcat atcgatttgc
     6241 atgagtgagc ttaccattag ctaaatatgt actgcaatca gtctgcgaat ctttcgcgta
     6301 accattacaa tttaaaaaca taagtgggtc agttccagat cttccaagat tataatattt
     6361 agccactaga ttaactactg atggtttgtc agatcccaag tttagaggat atccaaggat
     6421 tgttgtatta aaaccgtggc tagcaaaaca ttgttttgat cctcctattt cataagtcaa
     6481 tacatttgtt gataatttat tcagatgcgc atgtgtcatg gtatgagatt caatatccat
     6541 tccatcttta tttaactgtg ctatatcttg ccaattcatc caatatggtt gtgttgctac
     6601 tttaccacag actataaaga aactagcttt aaatccatat ttatctaaga ttggtttagc
     6661 atatatcaat tgacttttat atccatcatc aaaatttatc atcactattt tattgctact
     6721 accaacacta ccactgatgt taccgcccaa aatatagctg gctgcttggg aggaataaac
     6781 gggcgtggcg gaagaggatt gattatgaat tccatttaaa tttggtgcgc taccacgact
     6841 actactgccc ggtattccgc cactacttga gggtgtttgt gacgatgact tacttagggc
     6901 catagtcgga aaagaactac cgatgattag catcattaca gtagtgatga caacactaat
     6961 gctacaaaat gtctgaaaat attgacgttt cttaagattt gacaggatag ttctaattag
     7021 tttatgacag acagatttaa taatcattgc tcgatatatt ttcatttcgg aatggttctc
     7081 caatttcttc tgagacatag tatatagatc aagggtggtg gctataacca atgaagtagc
     7141 gccattatgt ccaatccgag taataggagg aaggtgatag aatcagaagt ttacgacgaa
     7201 ttcaagtatt tatttctgaa ttttttagat aagattacct gtaagctgag tcaaaatatc
     7261 atcgtatctt ctatttttct tattgtttga aatttctgta aattgtagtc catcgatatt
     7321 tgaagtccat tcattcatag ttttaaccgc tgcttgacga gagaaagtat caccatatta
     7381 agcaaaataa atattccgaa aatagcgact agagagattg agaatagcaa cagaaaggaa
     7441 ataaaaagac tttaccaaat gatatttcca tccatactag ctatttaaaa ggaattgtgt
     7501 gctatttatg gctggacatt ttgtacatct acttcctaac cgaaggtgaa tgtatataac
     7561 tggaaacctt tgccgcttac attgattata atggaatgag aaccatagtt gttgtatagc
     7621 gccaaattat atagtctttg tccatcaagc acgaatttgc catctggtgt caagtcctta
     7681 cctaatgagc tgtttaatat ttttgtagcc ccgtcttcag taactattcc tagttgtccg
     7741 tgtcctccag ctactatatt cacagccctc gcgttgtatg tcaagataat gtgtcctgtg
     7801 ttgctttgca attccatatt atcaggatta ttcttccatt gaccatcaag gtatactata
     7861 tcaggattga aattggtgtt tggcggaatt gagtaaggta ttgtttggtc gggttggaag
     7921 ttttgggggt ttccaagagg tgctcttgca aaggaatagc caagataaat ttccggtgtt
     7981 atttgttttg ttaaatcgac atatgcgagg ctgcctggat tgattgttgt tgttgattta
     8041 gctgtattga agcttatatc cttcatacca ttaagagctg ctccttcagc aagcaacgat
     8101 tgaattgcgc tttctgttgt attataatcg ccctctccta tgtgatcatg tctgatgtat
     8161 ccttgagtat caaccaaata atctcttggc cagtacatgt tgccatatgc attccaagta
     8221 ccgtgattac tatctaatat tacaggaaac tttattccca atttctgaac tgctgctttt
     8281 acattgtcat agtttttctc aaattcaaat tcaggtgaat gaactcccac tattacgaag
     8341 cctttgtcag catatctttg ataccaatcg ttaagatgag gtaaagtcct gatgcagtta
     8401 atgcagctat aagtccaaaa atcaacaagc actacttttc cttttaaaga tgcaagtgtt
     8461 agcggagcat tactcggcgt atttatataa cctgcaattt gggcaaattc tggtgctttt
     8521 tgaaattgtg atttatcaat ttgaaggaat tgggctttat caagctttat tgatttgatt
     8581 gtaccgtttg ttgtagtagc agctgaaaca actttttcta gttgttgctg tgaggaagaa
     8641 gaagccttat tgaggcttgg actgttgaaa tatactccaa acccaacaat tatagctatt
     8701 actgcaattg ccattgctac cgcgctaacg ttgtctctat tcatatttct cgtcttccct
     8761 ctctcatctt aaattccact ctggatgtta atgatttgat ttgcaagtgg gaaattagct
     8821 agcagaatta actggttggt aaacaacaaa acgcctacta caaccagtat tccacccatt
     8881 accaagttga agtatttgag atgtttgacc attcttctaa ttataccagt agctcttgag
     8941 aagaaagcac cagtaatcag gaaaggtatt cctaatccta tagaataagc caacaaagaa
     9001 ttgtatgctg ccgcaggatg agtagctgcc aatgcaaatg tgctacccaa gattggtcca
     9061 acgcatggag tccaaccagc tgcaaatgct gctccaaaga caaatgatgt aatatagctt
     9121 gttttaaatc ttggtagcct gcccagtgtc atctttttct caaagtttag gcttcgtatc
     9181 cttgtggtga gaatcaggta tgcaccaaat cctattatca caattccacc aacagatgta
     9241 aggtaatgtt gaaacccggc tccatataca gagagaacgc tatttagaat tacacccaat
     9301 actgcaaaaa taagtgaaaa gccaagtaca aagtaaacgg tgtttagaaa aatgttaagt
     9361 ctagttcgcg taagtatcat cgttgatgag gactgctgct gctgttgtcg ttgcttttgc
     9421 tgccctcgtc caccgccatc actattactt tcgatgtttc ccaccttttc attagaagta
     9481 atagtagtag ttgaagtatc ttgtactgag ttctttgctt catttatggc tgttcctgaa
     9541 agatacgata tgaaaccagg aagtatagga agaatacatg gtgatagaaa agaagccagt
     9601 cctgcgcctg cagaaactaa aacacttgtt tctaatacca tcttatttca tactaccctt
     9661 cgataacaaa acattcaccc ttgtattaaa tatatttcca atttacatcc aaatgaattc
     9721 caaaattaaa cagacacctg atggtattgg agtataaagc gagtacccat actgcctggg
     9781 actcggtgca aatgaaaagc tagggcatgc aacgatggca ggtcctttaa acgtccgata
     9841 aagcaatact gcatcatatt tctgtttttg aaagatatgc tttattgtat ttattgactc
     9901 tagcgaaaaa agtagagtat atcaaataac aatccaacaa gatctggagt atatttggat
     9961 aaaagtaaat aagagaattc ctactaccgt ttctattcta caatgaacag tttggtggaa
    10021 ataagtgcag gcgtagcagt agtcaacgtg gcactgttga tagctttgtt aactgtgtat
    10081 gctaaagtct acaaaagtac aagagctgta tttactattg gtttaatgtt ctttgctggt
    10141 atgctaatgc tacacaacat aattgcagtt tatgcttatt tttcgatgga accactgtat
    10201 tcagtagcaa tactgccata tttcgtagtt gtacatataa ccgaattagc aggaatagct
    10261 gtactgttga aagttaccct atagatagac actccttaaa cgtagtatgg atttgctcgt
    10321 ctagttgttt tcggtctgta actattacaa taggtgggtt tccgaacttg tacattagtt
    10381 gtgttgtaag ccaaagcatt gaaagagatt tacctgaacc ttgagtatgc catataatac
    10441 cacctttatc agaaatattt tcttcaagat tcagtctatt tacagacttt gttactgctc
    10501 taaactgctg gtgttttgct attttcttta ttctcctatt gtttacaact tcatatatca
    10561 cataattttt cagcaaatcc agaagatgag attttgttag cattccagca attagaattt
    10621 cttgctctct tgctttagta ttgcatgttt tctcaatttc ttcttcagtc attggatagg
    10681 cctcggacca tctggcataa tgattcgcgt cagaacctat ggttccatgt cttgcgagta
    10741 tgccacatgt tgcgactaga aaatgattat aaaatatgag tttgtcataa cctgagcccc
    10801 tgctctggta tcgttgaaag ttcttttcta ttgcttgttc aataggtttt gcaataaatg
    10861 gagatttgca ttctattatc acaagaggaa tgccatttac gaaaataacg atatcaggaa
    10921 atatcggctc cttcagacct tctagttgaa attgattggt gaccagaaag tcattgtttt
    10981 ctggattctc aaagtcgaac aatctgacag ttttttcctc gttcccctct ccaaagtttt
    11041 gggttactgt aataggttct agaccaccag atctcgataa tcccactagt tttgctcgaa
    11101 tcttttcatt agtgtccatg ggatcaagtg tataagggaa ataagattca ctaatttgcc
    11161 ttaaggcttc atagactccg tcctcgtcaa tcatgggatt ttgcctcctt gtaactgcat
    11221 tttgtgatgt tgcttgaagt ttattccagt taatatagac tatttgctga tactgaaggt
    11281 aggccaatcc agcaaaaaat aggcctacta ctactgccac taattttacc acctttttca
    11341 tttcatatcc taaaaaaaga cctccaaaga agccgccacc gagtgtagct agagttgaag
    11401 ataaatgctc aagactcatt tagattgcgg cacccatttt ggtatcctgt aatgtttaga
    11461 caagacaaaa gcaggttcac cactagatct tgtggcgagc attgagtaat tgatctgctc
    11521 ataagacatt tttgctacga tagtgtctac acttttccaa agtagtagca ttacctgttt
    11581 cattaccata tcgtttagta tcaacttata tattaaggta atgaatatgg taatgcgtat
    11641 tagcaagcta tactaatgga agacttataa tgtcactgtg tgggagaaag aactacatta
    11701 gcaaaggcat caagtaactt tgcttcctta cgaactgttg tgcctatgag tattgtaaag
    11761 caatggagtc taaaagaagg cgaccagcta gactggtctt gggaagcacg gaatggagaa
    11821 atgattattg taattaagaa ggcagaacat attgatagtg cagcaggtat aagatttgac
    11881 gtacaaagga gtggaaaaag gaaaaaagta gcaaaaggga agtaggagtc tctatagtta
    11941 tttagcagtc ttaatctcaa cgttctattg taatcattct gaaacatcac aaaatcatta
    12001 gacacatatt cgtgtcagaa actttcagca agtgattgga acccagctgc cgcgacggct
    12061 gttatctgaa ggatcgaact ctgtgagaaa tacccaccgc catatgtact ttagaaaaaa
    12121 taatttaaat ctagaaaatc caactaagaa atcctgtcag aactagggtt cagtccattt
    12181 gcagaatgtg aatttatgat attttctaaa agttcgagag ttaccatgcc caactatcta
    12241 ctttaataga actcaaccct gagaaagtaa ctatgccaga tactaatatg ggaacagtct
    12301 atttgtgggc atccgaattg gccgacttat ctcagttgaa agtcacaatg gacattattg
    12361 agaggactag gttggttctt gaaataaatg aaatgctcgg taattatatc aataatgatt
    12421 gtaaatccgg tggcaccttg caggggttaa acgtagaaag taagtaactc aaatacagta
    12481 gttgttatca tccgaagaaa ctggaacaat cattgcaaat actttttccg tcatgagata
    12541 atatcataat ataatattct ctacaatatt attagtaagt tatatccaat ttctccagtt
    12601 ttctggttta tgatgcagat aggtctagag tttgtttcaa tatctcgttt tagtagtctg
    12661 tcgatttcga tatttcttgg caaatattcc tgtaacacag tgatttcaag tcctatcctt
    12721 tcatattcgt aatctaccgt tctgtttgtc gaatccaagt ttattgaaaa aaggaataat
    12781 attcctctct acatgatgct caatttccac gtgatcaatt gaagaatacc cttcatcaag
    12841 agtgtgtgat tgaagacgta agacttacat aaatcgaaca tgcagtatca ttcacgctcg
    12901 tagattgata ccaagctaga gagttgaatg ccatcggctc atactagact gcaatatgcg
    12961 ccggcttaca gacagagaaa cgctagatta ctttcggcta aatggaatag aaatttcact
    13021 tgcgggaaaa cacacagaaa tggatagcca gacttgccaa atcaaaacgt tcatactata
    13081 ttgctgagta cggagagagg atagacgaac tttatatatg cccgaaagag ttgtggaaac
    13141 tatgcatcaa cgaaaagagc aaaccgttcg tggcagtagt gtgtagtacg tcagttcgaa
    13201 cgcaaatgcg tcatcttcag tactacagcc ttggctttta ctgctcataa tatatggcat
    13261 agaaaagaaa taatcagcta ctgcgtataa ccaaggaaca catcgtcaga tagacgtgaa
    13321 gaatttctag atcaagaata ttaacctctt cccgtataag atgatggttg catatccgta
    13381 aaaaaccaga aaatcgaaaa ggagatacca gcactgctgc tccgataaag agatcccagt
    13441 cacccaaaat actactcgta gacgatgagc ctgacactat attgacattg agaaaaggac
    13501 tagaagtgag aggattttca gtagatacat atgacgatcc tttattggca ttatccaatt
    13561 tcaagccaaa cttttatgat ttactgctat ttgatgttaa aatggcaaaa atgaatggat
    13621 ttgaactata tcagaaaata aaaaagaagg acaagaatgt taaggcatgc tttattacag
    13681 cacaccaaat attctatgaa tcgctaaaaa aagaatttcc aaatatcaag gtagactgtt
    13741 tccttatcaa accaattgag atagaagatc tggtaagaaa ggtaaaggat caaataaaat
    13801 ccctgaaatg aacagaacta acttctttca cttcaagata ttttaattgt agatgctgct
    13861 tcaatatgtg atgttctttg gcatcgctac ctctatccct tcttgaaccc catcaccagt
    13921 tagaatcaga gaccttgtat ttgtgcctat tctcgcacca ttagtactta acttcatctc
    13981 acaaggtaat cctgcgatgg ttatccctgc atcagatcaa gacgcctcag cagtttctaa
    14041 atatcttatt cctcatgctg acgatgataa aattagaaaa tatttaagaa tatttagtgg
    14101 cggaagtggc accgaagcta ttcctacagc ttcatatctt gtgtccaaaa cagaaaagac
    14161 tttcgctgat acctataaca attggacaaa agtctatagg gaattgaaag aaggaacaat
    14221 cggctgcatt gttacccttg actatagctt tgtagaacta gaataccaga acgaaattca
    14281 atcaattctt aagagcataa tagagttatc tcgtcttatc agactttcaa atgatttact
    14341 tattatgata tcaagaccaa gttatagatc tttagacgta atgatgaacg ttagtgacat
    14401 gcacatgaga atatttgaat acgatggagc aacaatgctg gcagcaatta aacctcaatt
    14461 ctttttgttt aacatacaat tagaatatac ccaaggattt ccaactgctg tacttagaga
    14521 gagcacatga agaaagtaat gatgatgata gactaaaagc ataatcagta ataatcttgt
    14581 ccactcgcaa taatgtctta aacttaaagt gtcaacttga agcgtaaacg aagctataat
    14641 aaagaagtac caagttgaat actgcagcag tagtatttgc tccaacaatg acaagatcat
    14701 tatgcactat cccataaatt atccaaagaa ttgaacctgt agtaaaaaaa gtcataagat
    14761 atctcgaaac atccttcatt ctttttgtac ggtatgcttt gacaatctga ggaacgaaac
    14821 tcaaggtggt cagaaatgct gcagatatgc caattaaaga atcatatcca aagttcattt
    14881 agagtttaat agagtctaga catcttgaat aaatattatg cgacccacta ctagaaattc
    14941 taaaaacgcc agtaaattga gctgaggtgt tttcgataac tgcctggtcg catgctattg
    15001 ccaatgttta ttttgcaccc atttgttatc gttattataa ttgaggttta aattctctgc
    15061 tcttggcttg cagtttcttg ctatgaatca ttatcgggtg gattggcata tttagtcaga
    15121 catattacaa taatgcagat ttgaaatgat tccagaggta cgagaaatcg tagaagtcac
    15181 tggaagattc gacatcctag taacaatgaa tgcacagtct cttgatgaaa tgcaccactt
    15241 gatttccgaa aaggtgggtc gtgttgaagg agttcaaaag acagaaactt ttattgaaat
    15301 gaggaagacc gcacgcgaaa taatctatcc aacctccata tcaaaataga gtatagctta
    15361 cttggaggat agatatttca tgatattgcc ttttatagca gaatagttaa aaaattgata
    15421 tgggcacctt gctcccgcaa atgagcgccg gtaacaggct caaaaattat tacgaactta
    15481 cgaagccaaa gatttggtac ctgcttgttt tcacagcttt tgctgcagca ctcactgctt
    15541 ctttcttatc tcacatcatt gttcacccca taacctgggt gctgattatt ggcggagtcg
    15601 ctgctggctc agcagccgct gacacgctca ccggttacaa cgaccgagat attgacgcaa
    15661 ttatggacag gactaagcaa agacctattc cgtcaggaag aatttctccc cataatgctc
    15721 tggctttcgg tttgatcctc gcggcaattt ccttggtact tgcatggctt attaatatct
    15781 gggcttttgc attgatgggt tttggattgt ttgataatat tatagtgtac agtaaatggt
    15841 tgaagaggac tagccgaaca aacattattt taggaggttt ttcaggcgcc gcccctgctc
    15901 tgataggcta tgtagcagtc acaacacata atattgaaat cggcttgatt atggccggcc
    15961 tagttttcct gtggattcca actcacattt ggagccttgc tttgcatgcc aaggaagact
    16021 ataaaaaggc tggtgttccg atgctaacag tagtttctgg cgagaaaaaa tcagtccgca
    16081 taatagcagg gactacatta atgatagttt tgttcagcat attaccattc ttcaaccatt
    16141 tcgggatgat ttatctggtt acggcgagta tctccggtgt aattatgcta gcgttatcaa
    16201 tttggctatt gttcaagcca agcgagagag tatcttgggt agtattcaaa gtcaccagcc
    16261 catatctggc cacactgttc attgccttca ttatggattc attgttccgt tgataatccg
    16321 tcctttccga gtcagggtta aggcttcata tatgtttgtt actctgctac acgcctccaa
    16381 tggcagccag ctggtcaacc ccgcgcattt ctcaaaaaaa ctgggactag tagtacagct
    16441 agcggtcata ttcatatcaa gatatgaaca acatcttctc gcactcataa agtactcgag
    16501 caataacaac atgcgcttcc aatgttattt ttccaataga gtatgagttg tcaaggaact
    16561 tacttatgtt gtcagaaaag tgacctttcg ggaagcctcc tatcacgatt gcgcaccgac
    16621 agtcgtcgac aaagagctta ccggcaagta caagcttttg taaatcatct tatattctta
    16681 aataataatg aaaaactcga taaccaacgt tcttgagttc cttttgtaac caagtttctc
    16741 aaataatgtt tataattttt ggattcagat tataaagtat aattaatctt caaactaaat
    16801 aaagaactgt ttgttttctt ttatagatgg taggatcttt ttatcaataa cagttttggc
    16861 atctgaatcg atatcaaacg ataatagcag ataaaatttc ttgttcttgc tatcattata
    16921 ggcgatattt tcaaatgtta ttggaactgt tgcacgaatc agcttttcgt acttaccaat
    16981 ggaatattcc aatttgccta tatttgattc aaattcttct cttatagcag cacgaagaac
    17041 tgcttctatg gcatattgtg aagtctgctg ctttgtcaat aatggaacca atccttctct
    17101 gtattctgtt gcaattaggc tgccaaggtt atttgctata cctgcaaacc tgatggactt
    17161 atctgctttc agtatttgtt tgcaaaacat agaaagattt gcatttcttg ttgttgcttc
    17221 agtatcatct tctcgcaatg acatagtcat cgctaatgta catctgtaat ataataatag
    17281 cgtcagcgaa tcagtaagct gttatctcta tttgccataa aatttttcgt ctcttcttca
    17341 gtcattgggt cacccatacc cgggaaagca ttaattattt tcatcactct aacctaagag
    17401 actcatggat atatatctaa atcaactatt catatttttt ttgacggata aatccttctt
    17461 tttcttctct ttggcttcta cgtattcttt caatgacttc aaattatagt catacatatc
    17521 ctcaaaaaat tttacgagtt ccaaggttct ctttcgattc aatgaataaa acctgttttt
    17581 tccctgtttc ttttccgtta tcaaatctgc gtctttcaat attcgtaggt gagatgagag
    17641 agctggcaaa gtaaagttaa agtgttcagc tatttcagta gatacaatat ctctctttag
    17701 cagaacggag tgaaagaaga atgcaaaaat tatttttggg tatactgttt tctctcgatt
    17761 aaagccctat ccaacttgac tttcgcgacg cctagagcta ccaaaattat accagtaccg
    17821 gtgacagaac caatggtgat cattgtactt ctcgtggttc cggaaccctc ggcgattaat
    17881 attatcatac cagctatgat gataccgccg ttaattatca gaagc
//
LOCUS       DAOR01000315            2798 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_75084, whole genome shotgun sequence.
ACCESSION   DAOR01000315 DAOR01000000
VERSION     DAOR01000315.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_75084"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          951 a          552 c          478 g          817 t
ORIGIN      
        1 cagggctttt atcaagacta tgagactatt atttgtaact gtgcttaaca tactgattgg
       61 gactggcaac ataatggaat agaccccgac ttgttgctcc atcatactag gctttcttta
      121 ataaatagcc tctctttcta gtataataca tcataccaat ataaagcagt gcggatacaa
      181 taaaacttgg aattgttgcc ccgatgggag gccaattagg aatataaatt ggagataacg
      241 aagataaaag ataatatagt aatacgccga tagaccatgc tattatagca ggatatccaa
      301 tttttgaagc atcaccgtta gaagcactat acattataga ctcggtgtat ctgcgacgtt
      361 ttacaatata ataatctgac aagacaactc cgaataatgg tacaaaaact gctccaatta
      421 aaagtataaa cgtttcgtaa tttgcaattg aaatagtcat agcaagaaca gtactgagag
      481 cggtaaatcc aattatcaaa taaagctgtt taatttttct aaaaatactt tgtgtagaaa
      541 ctgcagcaga ataaacatcg gcaaatgcat tatctgcctc atccacaaga agtattaaca
      601 aaagaaatcc aaagaatatt gattgtatgg ctgcaattat tcctacgaca tcggaaacac
      661 caagcattac tcctccaaaa taaaacaaga tatttgtcag tgtaaagcct ataaatgtac
      721 ctttaaatgc acctttactt tcctttgcaa acctgttata atcagctgca agtggcatcc
      781 aagatattgg catagctatt acaatatcta atgctgagaa gaaagacatg ccattacctg
      841 acgacgataa tacttttgaa aggtttccta ctgctaacaa atgtataatt ataattattg
      901 atgatgcata ggatatccat atagcaaatt ttcctaacca gttttttacc actgtgagtg
      961 gaccagagat accaagaagt gcaacaaaag ctccaataat aatcgtccaa acatagaatg
     1021 gtatcagttt gcctgctaac atttctgcag cctttgacat aatcataatt tcaaatgttg
     1081 tccatccaat aagctgcatc acatttaata tggctggaag gtaggctcca tgaacaccaa
     1141 acgacggcct cgtgcttatt aaagaaggaa ctgcatgatc acttccaatc ttacctgcaa
     1201 gagctagaag taaagaacct gcaaccgaac caactataat tgcaaggatg gcatcactaa
     1261 acttagctcc agtaagaaac gaacctgcag agaaaactag caatcctact cctagacttg
     1321 accagagaac aaagtaatcg attcctcgca gaaatctgta tcgtggtgga acaggttcaa
     1381 tgctccattc aggtggattt cttattgata ccatttattg tattaagaat ctacagataa
     1441 gggcctgtat atatctgatc caacacatac atacacaagc ctatatagtc taccaagact
     1501 atcgatatct cctcatgatg ccatacttta tcataggagt attcattttt cattggcttt
     1561 ctcatgaata tttttataac ctgtataaag ttagaataaa gtatttctag atgcttcata
     1621 ttgcaatgac caatcaaatg attaaaagca gccagtctag tctaatctaa gcgagtataa
     1681 tctaacctcc tccgatatta tattcgtcaa ccagggtttc ttgtggtcga aagaaatgtt
     1741 tgtaatactc tattgacttt atctggatat tgcacgaata atgcatggcc agcatctttt
     1801 atctgtacaa gccaagctcc agggattttt ccagctataa ttaaggaatt ggctatcatt
     1861 atcattattt aaaaagaagt cataataact aacttttgga tgaggtttaa ctataaaaac
     1921 ctcaaataca agtatcagat tggtagtatt gcaagttgca aagagcaaga agtatgatgc
     1981 tgttgttcct cctaaaacaa gtattctaat actacaaggt gagaatgata cacaaacccc
     2041 agtacagcaa acatttcttc tacagcaagc attgatagat aaaagacatc cagatcatac
     2101 attgataaca tatccaaacc ttggtcatgt gttctatccc tcatctgaat ggacgactgc
     2161 ctttggacca atacaacaaa atgttttagc agacctttat tcatggcttg aagctcattc
     2221 tggtctttca cattccttcg ctacaactac tgcctccact acaggagcaa atacaagttc
     2281 attgaataca agtacgactt ctccttcaag ctcttcaagt aaaagatgat attaaagaaa
     2341 atccagaggc aaccaggata acgaatggcg tcttaacgac tttagaggct atacagataa
     2401 aatctacata accgcggtta agaataaaag ctgatcagat atctgctatt tagtaggaaa
     2461 tgcatactaa tctgggttag tattcttgtg ctattaagtg catatgtaga atgtgatttt
     2521 ctactttttt cggattaaca actcgccgac aatcctagac taccttaata tccaacctcg
     2581 caaggtcatg ttcgacaggt tggttggctt gcaacggaac gcgtatcacc tctactgtta
     2641 tcgtatgatc tccgctaggt aacggcttga agaacagttc atagctttcg tccattgcca
     2701 gcccgcagca aggtggcttt atgctaaagg cattatctga agagcgtatg gtgtaaatga
     2761 aaggctgaga agtgcttatc cgcttctaca tagataat
//
LOCUS       DAOR01000316            4643 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_89178, whole genome shotgun sequence.
ACCESSION   DAOR01000316 DAOR01000000
VERSION     DAOR01000316.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_89178"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    356..593
                     /estimated_length=238
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1262..1423
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1368 a          752 c          786 g         1337 t
ORIGIN      
        1 gtactacaaa ttgttaatcc caaacttaac aaagactctc aaactaaaca atattcttcc
       61 aatgaaaaaa ttgtgaattc gaaggtagtt caaaatgcaa ttgaaataaa aaataataag
      121 tcgattaatt cgaagccagt tgctataaca agaactattg ctttagcaat aatcctgata
      181 ggttcggtga tcgctacagg agcatcattg ttagtgtata caccgacggt aaataatcaa
      241 gaaccaggca gtccattaat ctaccatcca gcagcattct cccctactat aaacaaattt
      301 cagtccttaa atacatcaac ctctgcacaa gatgcaggcg taaattcatt tgctgnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcagg
      601 cgtaaattca tttgctgtgg caactacggt tgatcctgga ggagggtcag tcatcgataa
      661 tgtgtttgtc gtagattata gtgataatac tttccataga atcatatttg atattggaac
      721 tgaaaatgtc tctggtgcat cgacgacgat ttatctgaga aacgcatttg tggtcttaaa
      781 catttgactc attgttcgaa caaagacgta ggggaagcag ctcagcgata acacactttg
      841 gcagctattc gggtttgtca aacgaaacgt tttgaagcaa atttgaaaca cttttcatct
      901 accctgagat cactctcctt tgaatttgaa acaaagcaat gacgtttccg agtgggccat
      961 aacaatgttt atcattaata acattaatag taaatagtaa tccttatctc cgagaaaaaa
     1021 atagaaatgc tggtatttct atacgtgcac tttgcacaat atttgacata tatgtattga
     1081 tttagcgcaa tatatgatct attaatacat caggattatc caacggatag agacattcgt
     1141 gacaatacat aaatactttc ttgttcaata aataatatgg atgggagcgt gaccatcttc
     1201 tgacttgtaa gggcacatgt acacgtcata aggcaagaaa gcccgtaggc tctggtcgtt
     1261 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagccc gtaggctctg
     1441 gtcgttacgc tagcggacaa aaacgttgtc aggtctgcga ggtgtttata gagtgggatg
     1501 gcatatggtg cccatgttgc gggtacagat tgagaactag gccgagaaat atgaaataca
     1561 agacaaaatt gcgttctaag acttttgaag tacaactaca ctatgaaccg cgtaatatgt
     1621 aaacccattt acgtttattg catctgcaga caaaagatct gactatcgaa ataacattct
     1681 ttattaacag cgataatcaa ccagaggttt ttcacagatt gataattgaa ttaacgatac
     1741 agaccttgat ggcatcatcg ataggtctgt cgacctatca atgctatcga taatttaacg
     1801 atattaaaat caggtgaagt aataaaagac agcgaggtaa atgaaaatta cagctcggga
     1861 aaaaaggtat tcgggtcacc tttgtgttct acaagaggaa ggtgctgctt gtattagtgg
     1921 attgtggagg tataatattt gtagtgaggg ttggactttg tggccttcct cccgggaatt
     1981 ttttgaggat atgtatgact ataatttgaa gtcgttgaaa gaacacgtgg aaagcaaaga
     2041 agaaaaacaa gaagacggac attcaatgat agcaatatga gagaattttt agccccataa
     2101 atcgtaggaa tgtatatgtt ttcgaggaat tcttgtaagg attaaatcaa agtagaagat
     2161 tctttagtat tgtcattgga caaacacact ctggcatctc cctcaagttt tgttatagcc
     2221 tgccggtgag gctgctaaag cttgagaccc ttattactat ctaataattt tgattgggcc
     2281 agcaaattcg gtatgaatgc ttttagaaat agtaccggta acctatcgag cacataatca
     2341 cccgcgattg cagaccatat gaactgaatt ttcagaatgg ccttgataaa agaagtgttc
     2401 tgagtcgatt tcattataat ctatcacata tatatggatt aatagttaat agtttaaagt
     2461 gtgacgaatc atgagtaaga ctagcattgt gattagtgag ccagtagcta aaattcttaa
     2521 gaaaaggact tttgcatttt tgggaacagt gatgaaagac ggttcacctc atgtcactcc
     2581 tacctgggtt gatgtggagg acaatacaat tctggtaaac acggcagagg gtagaataaa
     2641 acaaaaaaat atttcaagag atcccagagt ttctatttcg attgtggatg atgaaaatcc
     2701 gtacagtatg gtaacaataa aaggacgtgt catagagcaa tccactaatg gagctgatga
     2761 gcacattgac aaacttgcaa gaaaatacct taatattgat aagtatcctg gacattcccc
     2821 acaaatcaaa agagtaattc taaaaataaa accagagaag gtgttctata ttgctccaag
     2881 gtatgagcaa tatctgaaga aaaagaataa ttaaaatcgt ttggtcttgt gatatgattc
     2941 tttattacat ttgggaggca tcatccatca gtcggaaatt aaatcatatg atctaactct
     3001 ggtttgtcaa gtacagtatc ttacaagaat accctggcct gaccataaat agaaatacta
     3061 tgtgtatgca gcaatagcga catcataatt tttcaacacg ctattcttaa attctattcc
     3121 tatttcgttc tcattaagca atatttcatt tctggtaact cttattggat agttgttgta
     3181 gtacatcatc gctgtgatga atagttctgt attttctatt gtgatttcaa ctgttgtgtt
     3241 tctttgaggg ttctggattg acaagaattt ttgagggctg taatttatgg tccagctaac
     3301 actattttct gatacccaac tattttctac tactgttgca atcagattgt ttattttatc
     3361 gaaaaaattg atgtcaagaa ggaaatactt cccattatct ctgcttactg ttataaggtc
     3421 aaaatcatcg attactaata ctcggtgagt gttaataaac ttggattttc ctacatttat
     3481 gactaactct tgcgattcta caaaaaatgc atcttgcgga tgtaatttat tatgagggtc
     3541 tctttttgca tcacgtaaat atgattctga gagatcccca gtatcatatt tgccataatg
     3601 tggggggcaa agagatatca tattctcagg aaggaatgct tgaatccgat tatatggaac
     3661 tatatgagca tactttagta aaggagaacc acatagagca cacccaaaaa aacattcttt
     3721 tctgagctgt ctttggatct gtagagggac atttctcccc aagtcaactg acatatataa
     3781 aatggctagc gatgtgcatt atataatgtt gtcattcaac cccaaaacgc gcgcaaattg
     3841 gagcatttga agttgtcata ctcttagcag ccaataacaa atgttttgga ataatgacca
     3901 caatattctt tactagcccc gcttatgcta atgaccgaat gatgataatg ttgcttttac
     3961 tgtttccagc tccccgcact ttattaaatc ataaatcgta atctgttttc tgaacgattc
     4021 aatatctgca tgtaaatgag tcaaaaaagg atgagggccg gtagctccaa tgatcttgcc
     4081 atctctgctt acgccgtttc tatacagtga taataatgct tgccctgatt gatgtccttt
     4141 tacatcattt ccgcatacta ctatgtaacg cagctcggga tgttttaatg taaatttgat
     4201 caatgtatct atccccttgt tctctgagag taatcttcca acaatcaaaa ttctattcat
     4261 cacaccttct gttctagcaa tagtttgaag tagatccata ctagacaatg tgcaaatagc
     4321 tacggtcttt ccttttccta tgaaatactc atctttaata ggaataagaa tttcacataa
     4381 ccttccagca atattctcta acttctttat gaacgtaata ttagccattt cgttacgtca
     4441 aaataggaga tacgttggat ctataaaact tctgtgtcca cagtttttgt tttactacta
     4501 tggtctacca tcttcgcaat atcctcttta gtatcttatt atcatcagtt gcctttacga
     4561 atctaatatt atctttattc ctattgagcc atggtagatt agaagacgga aatactaact
     4621 catttgatat tacaatgcct tta
//
LOCUS       DAOR01000317           15626 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_41872, whole genome shotgun sequence.
ACCESSION   DAOR01000317 DAOR01000000
VERSION     DAOR01000317.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15626)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15626)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15626
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_41872"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2916..3086
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3587..3881
                     /estimated_length=295
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8332..8532
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4578 a         3078 c         3012 g         4291 t
ORIGIN      
        1 aggagtgcgt aattcatgag ctgccacatt gatgaactct tgctgcattt tatcgtgaat
       61 ttttagttct tcatttaatt tacgctcttt ccatagcagt tcaaatacag atctcagtga
      121 atcaactcca actctgttat ttgaatagac agtaaaacca attgcttctg aaaacttttc
      181 ggctttgggt tctttgagtt ctgctcttaa gaacttttca ctatcgatta tgtacatacc
      241 atataagcaa ttatctccgt ttagaattgt gacagctggt gcctgctcaa agattttctt
      301 gagaatttct gagttctcgt cagacaaagg acagattatc tttacggttg cgcccttttg
      361 tgatgcctca atgatattag caataaatcc caggcggtcg accctcacca ttgccctggc
      421 attgggaaga aggattagag cctccttcct tacagatttt gctaacttga gaagaatctg
      481 gcttactact tcacgatccc gatctgtaaa gacttcaaaa tattccggtg ttgtaccttc
      541 ttcaaattgt ctaatcttct cttccgctga agttgacttt ttccataggg tttcaaaaag
      601 atattgttgt tgttctacta cgtcctttac gttgctatag ataacctctg gtacgggttg
      661 tgcctccctc aaagttgatg tagctacata ttcagtttca cttaccgcga agttagcttt
      721 aactccgtct aaatgacgaa gctcatctat attttttact agctctttac aataacgagc
      781 attttcctta gtaatctcgg taatctgtct tactttgaca cctctatttt taaggttatc
      841 tagagctctc tcgtattcgg cccccatggt aacaggtagg gtcggataag gcgaacacgc
      901 actaatcccg cttcttgcgt tggataggaa tcggaatatg gtattagtcg tattctcaac
      961 accatctaag acttctgtct tctcggtatg cttaatcggt gaggatggtg ccgtagttac
     1021 caatgacaaa atgtcaagcc acccctattt aagaaatttc taaaacttag gtcgtcgttt
     1081 actatactgc ccaagccacg cgaagagatc ccatatcaaa tttagattgc taactcgttt
     1141 gatcggaggt gatatacctt aacttgacac atccgatgca taaatagaaa agttttaatt
     1201 ttatgatata tgaaaaccct taagaaaggt attacctacg cttaaataag ctagcagaca
     1261 aatatgtttt ctgagctcac cccaatcgtt agttattgca ttgggaggaa atgccttact
     1321 gcgaggtgga caaagtggaa gctttgaaga acagcatgca aattttcaac ctgctgcaag
     1381 taaaatagca gatttagtcc aaaaagacta caaggtagta gtaactcatg gaaatggacc
     1441 acaaatagga tatattcttt tgaaaaacga gatcgccaag aagtatgcac ccccttttcc
     1501 gattgatgca tgtggagcgc aaacccaagg ctggataggc tatatgattc aacaatcgtt
     1561 aaaaaatata ttgaatgcaa gagggttgga aaaggaagtt attacgttga taactagagt
     1621 taaggttgat aagaatgatc ccgcctttca aaatccaaca aagcctattg gtccattttt
     1681 caataaagat caagttgcct cgataatgca tgaacaacct gattggatta taaaggaata
     1741 cgagggtaaa ggattcaggc gagtagtgcc atctccgatg ccccttcaga tcattgaatc
     1801 gaaggcaatt cgtacaatgg tcgatgttgg attcctagtc atcgcatgtg gtggtggggg
     1861 tataccagtc atagagaaga atggagtttc aactgggata gatgcagtaa tagataagga
     1921 ccttgcaagt gaaagattag caacactcat tggtgcaaca aagttaattc ttttaacaga
     1981 tgtagatggt ctgtacgaca actatggtaa gagtaaccaa cagcttgttt caaagataag
     2041 actcaatgaa atcaataaac ttaagatagc agatttagca gagggtgata tgggtccaaa
     2101 agttcgggcc tctttaacat ttatagaaaa tggcgggaaa gaggctgtaa ttggaaattt
     2161 gaaaagactt tccgagtcat tagacggtac tgctggaact cacattgtac cataacgatt
     2221 cgttacgcta tagcttcttc gtacttcttt agactttcaa gagcaagacc cttattccta
     2281 caaccatcca catagttagg atgtatctct ctcgctttat catagcatgc aatagcctct
     2341 tcgtacctct gtaaattagc gagaccaaca cctttgtcat tccaaccatt ctcattcttg
     2401 gtgtaaaaga atttaacgta gaaaaagcaa ttggattcga agctaggcca tacagagcta
     2461 aaattgcaag gatgagacta aaaaaaagaa catcttgaca acaggatctc catagttcag
     2521 aaagataggt acaaagccga tttgattaag gcagatgtta tctttcattt gcataatgaa
     2581 gttgaagccg atatgaaaat gctatacaac cagaaaataa gaaaaggttc caaattaatt
     2641 aaacacgatt taccgctcat aggatacctg cctgacaagg tagattatcc tttttataga
     2701 atgacatttc cgctcagaag agcgaaaagt aagaaacgat gggcatcggt agtgttaggg
     2761 aagaaaaatg ccttggtcag tgatgaaaat tatataatat atccaaatat gaatttcgtc
     2821 atccgtttaa tgaaattgag ataaatactt caatcattca tttttttcgt cagagaatct
     2881 accaagaatt ttcgaggata attctccata ttcgtnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnngttt tttctcaaaa gcattgctta aactttgatt
     3121 gtttatattt tgttatagac tcaattcata tgtgaacaat aagatactcg tttcaatagc
     3181 aatgcccaat gagaaggatc tggaagttat gaatgaatct gtaaagacac tccgaacgtt
     3241 cagagtatgc tgtgaggtag ttgtgatttc tgctcacaga tccccagaac aaatgatcga
     3301 atacgcaaag agtgcgagaa aaagaggcat agaagttatt atagcagcag caagcggtgc
     3361 tgcacattta ccaggaatga ttgctgcatt tacaacatta cctgttattg ctgtaccttt
     3421 aaggtcatct tttcttgatg gattagattc tgttctttct atactacaga tgcctatagg
     3481 agttcctgtt gcaacagtgt cagttaacgg tgccagaaac gcagctatcc tcgcctgtca
     3541 aatgataagt ttgcacaatg aaaatgtcag acaccaactt gataagnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagaagcaa cgaatgttag
     3901 atcaaagtca tccatcacat aacatctttt acaacgcttt tatcgccttc tatactagtg
     3961 aatatctccc taagtagaac tgtgtgttag cgttaattag cataacttgt tttgtcctcg
     4021 atgcttactt tgctaattgc tatcttccta tttatacacg attcgcattt cccacagtgc
     4081 atacgatttt tacctgtgtc cttcacgccg tcagaatagc agctccatgt atcgaagatc
     4141 ttatcaccta aaatcctata gcctttgctc aacaattctg acttatccat accattaact
     4201 gcaggagacc aaacagttat ctttcgcctg aatcctaatc ttatcccgtc tgcttccgct
     4261 agattgagcg tcttagtgat tgattttatg aaagttggac gacaatcagg atactgtttg
     4321 tcgccaatat gtgcaccata tgctacaaga tgtgcactaa tgctcatggc ccatgcagtt
     4381 gcaattgtaa taaatactgc attcctcagg gggacaacta tactataatc aaaatgttcg
     4441 ggtaaatttt gtgtggtgtc tgtcagggca tttgttcttc cataaatctc cttcataaac
     4501 gcgatatcaa taattctatg atcatcagtg ttcaaaatcc ttgcgaactg ttttgccgcc
     4561 tgaacttcat gccttgcgcg ctgtccatac acaaaagtaa gcatgtaaat agtgtaacct
     4621 tcttctttca aatgggccgc cgcacaaata gaatcaagcc cgccgcttac tatgcagact
     4681 gctgattttc ttgtccttga agggggagcg tcagtgttgt atggcaatat aggttattaa
     4741 tgtaatactt tattatctaa atgtgcctgt atcggaatta tactaatcta gtaagcgacc
     4801 ccatagtaac catatgcaca gctttgatat gcaccgcttc aacatagcct tatcccggca
     4861 aaaggcatca aaaaaatcag acatgaatat tgttattcgt gattatgaat gacatgctgt
     4921 tcagatttta tgatatatga aattacacaa gttttttaat ttataataat attttattaa
     4981 tatgactaaa aaatttatta cttgcgatga ataaacagcc tgaggcgaaa gaattaacca
     5041 ctgtaacaac tattcagcaa ttatctaata ggatacagaa cggagaattg tcgccgtcgg
     5101 aattagttgg agtttgctta gacaaaatca agaaatttga ctcatccctg aatgctttca
     5161 tcactgttct tgaggatcat gcacgtcaag aggcagcgaa cgctgaaaaa caaattaaac
     5221 aaggaatcta tctcggtccc ttgcatggta ttccattttc aattaaagat gtcatctatg
     5281 caaaaggagt tagatgtact gcaggatcaa aaataatgtc tgactatatt tcaaaaattg
     5341 atgcaacttc tgttgtaaaa atgaagaaag caggtgcaat tctaatcggt acaaataata
     5401 cacatgaatt tgcatgtggg ataacaaatg taaatccaca ttatggttca tctaggaatc
     5461 cgtggagcac ttccaaatta tcaggaggtt caagtggagg ttctggagtt gcagtcgcag
     5521 catgtatggt acctgtatct ttaggaacag acactagcgg atctatcaga gttccatctt
     5581 cactttgtgg tgttgtgggc ttaaagccta catatggtag agttagcaaa tatgggattg
     5641 ttgacttggc cccatcgcta gatcatctcg gatgtataac tagaagtgca tgggatgcag
     5701 ctgctgtatt gcagactatt gcaggacgcg atccgctcga tccaactact gaagatagaa
     5761 aagtacctga ttatacaaaa attattgaag aatccaatag cgataaaatg tcaatcggaa
     5821 tacctaaaga atatttcttc gactacatac aaccagaagt agaagacgtc ttccatgatt
     5881 tcattgacgc tatcaggtct atgggcatta cagtttctga tgtaaaccta caagaaagtg
     5941 ataaaattta cgaatcttgg aggtctataa ggcttggaga atcagcagaa atacactcaa
     6001 aatggttaca gacaagacca gaagattacg gcgaagatgt actaaaaatg ttgatacagg
     6061 gaacggaagt atctgcggtc aattacatac aagctcataa gtttagaaag gaagtaagaa
     6121 atgcctttat gaaagtattg aagaacgttg atgttgtcat cgtgccaaca actattctta
     6181 ctgctcctag ttttgatgaa ccaacagttt ctattcgcgg caagaccctt gaagtctata
     6241 atgctctgag tagaaatact attgcgttta atagtactgg gctacccgca atcactattc
     6301 cagctggatt aagtaagggc aatatgccag ttggagcaca aatagtaggg gcgccgtttg
     6361 aggaagtaaa aattctttca ttagcatatg cattggaacg cgtaaataat taccctttca
     6421 aattgtctcc attttgtact gagtcatccc taaggacgga ccggaaatga atcgatagag
     6481 tcataatctg atttaacgca catggcagaa tctgaaaaca gaaagataac ctgagtccat
     6541 catcaattaa aataccaacc atttatgaag tcttttgttc aagccttgga ttaatgtatc
     6601 ataaaaatca gaaaagcgca taaatgaaaa ttgtcaaatt gactaatcat attaatacaa
     6661 cgaattttta acacgcttat ttatatctag ctataatcat cttaattttg caaatggttg
     6721 aggtaataga aggatatact gggagactag ttccagaaca actgaaacta agcccgctct
     6781 acaaacccag tttttggcat catactggtt caatacatca cctacaggag cataataata
     6841 agcaatgaat cgcataaaag atgttcagtc tgcaacgccc aataaagact ttatttctgc
     6901 aacaagagac gagatactaa ataaagtaga gaaggattac gaagaattca aaaaaacaca
     6961 tactttcgcg tttccaactt ggctatatgg tcccccaaag ggcaaactct tcaaagtaca
     7021 agttgaggac tgcccgcgtt ttggtgataa ggcttatctc gaatttgatt ccgccaggac
     7081 agcattcatg agcatcgata tgcaagttga tttttgcggt ccaaagggct atgtagacat
     7141 tatgggctac gatcttggtt taacttcggc acctatcaag acagtccgat atgtactgga
     7201 gactatacgg aatggaactg atattaaagt aattcatacg agggaaggac atctacctga
     7261 cctttcagac gcaccgtaca ataaactcct gaggagtaaa cttatcggga acggtgtcgg
     7321 aattggtgac attccgaagg gtggactagg acggttgcta gtaagaggcg aagagaattg
     7381 ggacataatc gatgatctat atccagttga gggagaatat gtagttgata aatccggtaa
     7441 aggcgcattt ggtcagagta atttaccgtt aattttgagg aatttaggca taacgcactt
     7501 agtaattact ggaattacga cggatgtatg cgttcatacg ataatgagag aggcaaacga
     7561 taatgggtac tggtgcacta tgctcaaaga cggtacaggc gctactgatt atgataacta
     7621 tcaggctgcc ataaagcaaa ttaaaatgca gggaggagta tttggatggg tgtcagattg
     7681 caataaattt gttcaaggcg ttagatctgc tttttttaat gataaaacga aaacaacttg
     7741 agcggaatca acgacccgtt tttttaatat aacaacctta gtcaaacgtg agaaacgacc
     7801 gcgcctaaac aagtcggttc aagagaagat tctcagaaaa cctctgtgtc gttgttttga
     7861 agagagacta actaaaaata attattatta ttgaagcaac ttagcagcga caccaagtta
     7921 caaagtctaa accaaacacg agagtgctat tattatctct ctcctctttg gtatgatata
     7981 gccagcgcag caggcgcggc gcttcgcttg tacagtcctg ccatgatggc tataggcaaa
     8041 agatatggca gcattaatag gaattctgag ggtacggccg ttcctatagc ttgcagtcct
     8101 aactggaatg cgtctaccat tccaaaaatt agggctccgc atactatcca gaatggtttc
     8161 caccgaccaa atattactac agcaagcgca atccaacctt gtccggcagt gatgttctca
     8221 aggaaattcc cagtggttac tagggccaaa tacgagcctc ctattccagc cagcgctcct
     8281 ccaatgatga ctgcgagata gcgtattctg tatacgtcta cacccaatgc annnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nngtatacgt ctacacccaa tgcatctgcc tgtttaggat tttctccaac
     8581 agtccttatc ttcaaaccga ggttggtttt gaaaataaaa atttgactca gtggaaccaa
     8641 caagaatgcc agatagacca aaggatcgta ctggaaaaga ataggaccga gcactggaat
     8701 atggcttaga atggggatat caattggagg tatctccctt atcttgggag ctattgttac
     8761 tactccgaat atcaatctgt ataaaagtga tgtaagtcca acgcccaaaa gccagatagc
     8821 cagtcccgct acgagttgat tggttcgtag gctgacactc ataaaagcca tcaaagcggc
     8881 cattaatgca gggccactca ttgccagtgc taatccaata aatatatttc cagtaaggta
     8941 cgttccaatg aaaccagtaa aggcacccat cagcatcatt ccttctacgc ctaagttcag
     9001 cacaccggag cgctcagcga tctgctctcc gagagctgca aaaagaatgg gtatgctcaa
     9061 acgtacacct gacgcgacaa tacccatcgc aaatacagca gtttctatca tttcacagga
     9121 attgcactac tcccgctctc tttgattatt ttcgttttga ttttccagcg cgtgaataac
     9181 tcccctataa gcacgaagat aagtaccagc gcttgtagtg tatcagcaaa tgcactaggt
     9241 atccccaacg tcctatacat cacctgagat cctgacatga gggcagcaaa gaaaaatgct
     9301 actactgcaa caccaacagg atgctgtctg ccaagcaatg ctatgatcac tcctatgaaa
     9361 ccatatccgg gagaaatccc ctctaacaac tgtagttgaa cgccggacac taggattgca
     9421 cctgcaactc cagcaagtcc gccgctaact cccattacta caaaagccct cttggccaca
     9481 ttcatacctg cgtatcgcgc cgctttgatg tttgctccaa cgctcctgat ctcaaagccg
     9541 attttagttt tccataaaag gatatagact gcaatcgcag ctaaggctgc tataatgatc
     9601 cctgcatgta gatgagtatg aggtaatata atgggcagct gcgatgcagg gaaaatctca
     9661 ggggtgtatg gattgaagcc aggcgctttt attggtccat gcagtaaata cgaaatgaaa
     9721 agaaggacaa caaaattgag cagcacagta acgattatct cgttaactcc aaatttgacc
     9781 ttaagtgctg ctggtatcaa accaaaggaa cctccaaatg caaatccaac agcgatcaca
     9841 gctattaatg ccaagcctgg tattgctccg attgctgtga taccgatcag agctgcgcca
     9901 agtgcaccca tgtacaactg tccttctgca ccaatgttcc aaattctgca cttaaacgcc
     9961 actgccactg ccagtcccac tagaagcaat gaggtggttt ttaccagagt atcacctatt
    10021 ccacgtatgc ttccaaatgc tcccttcagc atgatttgat acgcattaat cgggtctact
    10081 ccggccgcat aaagtataat cgcacctacg acaaaggcaa gaccagcgga aaggaaaggt
    10141 atagacgatt taaagttaat tgacctccgc tgaagtgtga tgactttcat tttttttcga
    10201 cgataccaga agttttttga aaaccagttt tgcccacatt tccccccatc aaaagcccga
    10261 tagtgtggac atccgcattt tctttatcag taaccgccat gatttttcct tcatagatta
    10321 ctgctattct gtcactaatc gacatcacct cgtctaaatc cagcgataac aataagactc
    10381 caactccgga gtctctctgc tttatcagct gtttgtgaat gtattctgtt gcaccaatat
    10441 ctagtcctct agtaggattg tgtgcgatca gcactttggg attgcgagaa agctccctgc
    10501 ctaaaacaac tttttgtaga ttgccacccg acatggatct aacttgggcc tctaatcctg
    10561 ttgtacgtac gtcgaaatct gcaatcactt tttctgcgaa ctttctcatt tcctgctcgt
    10621 tcaagaacaa tttccctgaa tatggtgcct tgtaccacct gtcaagaatt agattttgag
    10681 cgatagtgta ctccaaaatt agtcctgtgt tcagacggtc ttcgggtatg tacgccaaac
    10741 cccattccct gatttccctg ggagacagat ttgtggtagc tctcccatcc ataataattt
    10801 gccccccttg tatctttctt agacccgcta taacttcagc aagctcgctt tgaccgttgc
    10861 catcaacacc agctatccct aagatctcgc cttttcgcaa ttctaaagaa agatccttaa
    10921 gaaatagtat tttggaatta ttaagggcac gaacattttt tatctcaagt accgcttgac
    10981 ttttttttgc ttgtgccgcg actacttctg attctgttcc tcctcctccc cgttgtccac
    11041 tgatttgttg aacaacggat cgtcccacca tcattcgtgc taagtctgat tcgttggtat
    11101 cagaagtttt gacagttccc attagtatgc cgttttttag gactgtgacc ctgctactta
    11161 ccgccatgac ctcacgcaat ttatgagtta taaaaattat ggataaccct tgatctacga
    11221 ttgactggag cgcggcaaat agtgattctt cttcttgtgg agtaagcatt gaagttggtt
    11281 cgtcaagaat tagtatgtca atgtccctat aaagggcctt tattatctct acacgctgct
    11341 gttcacctac ggaaagatta cccacgatag cactgggatc taccttgaga ccgtattttt
    11401 ttgataattc cacaatcttc ttttctgcgt tagatgtatc taagaaaatt cttttcctag
    11461 attttaatcc gacaattata ttctccgtaa ccgtgtgagg aggggtcaac aagaaatgtt
    11521 gatgaaccat agcaatgcca tggttcattg cgtccaaggg cgacttgatg ttagcacgtt
    11581 ctcccctaac atatatctcc ccagtctccg gccgatataa tccatacagt atgctcatca
    11641 atgttgtttt accagctcca ttttccccaa gcaaagcatg tatctctcct ctagccagat
    11701 caaaatcgac attttgattt gctatcaaat taccgaacct gcaggttatt gatttcattt
    11761 ggacgagtgg ttccccgtct ttgatatcat acctctgttt tcctagttta agagcgtcag
    11821 tgttagtggt cacatcagca gagccttttt gttgtatgtt tttgaaaatg ccatgttatt
    11881 gctagactag acagtcccca aaactagaca gtcccccaaa aaaaatccgc tgaagctttt
    11941 ttttaatacg ttcattttta gccaaaccgc cggcgccaaa aaaattaagc ggtaggagtg
    12001 gttctctccg gtacttgaat cttgccagaa attacgtctt gggttaactg ggctagctta
    12061 gcctttacat ttgcaggtac tttagtgtct agactgtgga acggcgctaa atacacaatg
    12121 ccatctcctg gacctccttt acctgtttca attccaggtt tagtcagttt gccaacaaag
    12181 gtaccgtctt taacgctttt atacgcctgg tcgtatgcct tatccatatc tatgacgaaa
    12241 gaggtaagga ctgtgtttgg tgccaatttg ttctggtcag cgaccgctcc aaatgcatat
    12301 acacctttcc ctgcagccgc ctcaataaca ccatgaccag atgtatctgc tacgtgaagc
    12361 aaaaaatcgg cgccagaatt aatttgtgct aatgcggctt ctcttccttt agcaggatta
    12421 ttaaagtcat gcaagtatgt actaattacc ttcatgttcg gattgatgaa cttcgcgcct
    12481 tgcttatatc cttcggcaat attgataatg ttcggatatg tttctccacc cacaaaacca
    12541 attgtcccag ttttagacat catcgccgca agtgctccta gcagaaacga cccctgttgc
    12601 tgcatgggga aaattgaagc tacatttgat gcgttaacaa ggccagtcat gaccacaaat
    12661 ttaatatctg gatacgtcgg cgccaccttc acaacaggat ctccccattg aaatccttgg
    12721 gcaattatca ggttagcgcc tgcgtcggca taacccgtta gcgtagaccc aatatctgga
    12781 attgcaatac tatctgctga cgtaaggcca acaccgtatt tacttttcat tgcttgtgtg
    12841 gcattgaatc cgacggctcc ccaccctccg tcattaaact tcgcatcggt tacaaaaact
    12901 actttaaatg gggttgctgg ctgtgcatat gagtaataag gttgaatagg atgaccagct
    12961 cctgctgcta gtgtcaagac cactgctact gccgctacga ttcctaggca tgtaatagct
    13021 ttctcctttc ctatgatggt catagcgtct taaattgcgc tcttgatatt atagttttgt
    13081 agaaatatgc ttgccagtta gaatatatca taaaaaacgt aactaaagat atatgcggtt
    13141 tttcaaatat tcccatactt aaggttaaat acgctttaat gcatttgata attgccgaat
    13201 gaggttgtgc attcaatata tatgtcattc ccgagtctct ttgatgtctt aattaaagag
    13261 aattactaca aagactctct tcagctccta aaaatatctg atgatataaa acaaaatgat
    13321 ggaatactag acgcagctat agtaatggga actaagacaa acaaagaaat tcttaacaga
    13381 ttgggtttca atcttcctaa agttagccaa gctaaggata ctgatgtaat tatagctatt
    13441 atagcacgtg acaaaaaatc tttagattta attttatcga aagcggaggc attgctggaa
    13501 ggcaagcagc atggtgacaa ggagagcgcc ggagggagta tcggtactat caatactact
    13561 gctggcctgg ctgacagatt ccaagattta gattcagccg tagcatttat gcctgatgca
    13621 aacctggccc tgatctctat ccctggtgaa tatgtgaagg atatctgctt caaattgatt
    13681 gatgctggaa tacaccagca gctattcagt gatcatgtcc cgatagaaga tgaattggaa
    13741 ataaagaaat atgctagaga gaatggtatc ttgattctag gcccgggagc tggtacgtct
    13801 ataattaacg gcaaggggat aggattttct aacgcggtca aaatcggccc ggttggtata
    13861 gttgctgcgg caggaaccgg attacaggag gtttgtattc tactagatca ttgcgaggtt
    13921 ggcattaagc atgctcttgg agtaggtgga aacgacccca aggctgagat aggaggtatc
    13981 atgatgttag aatccataag aatactggat aaatgcgacg acgacgaaat caaagtaata
    14041 ggtataatct caaagcctcc ctcagcttcg gtcgagaaga aattcattga ttacgttgta
    14101 ggttctggca caagaaaaaa gtacgtaatt tgctttattg gagggtcagg gtccgcagaa
    14161 atagtaagcc caactcaaaa cggaagagtc attcaggcta atactttagc atcttcggct
    14221 ctggctatcg caaaacagat aggagataaa caattcaaga cagcattaaa ggaactcatc
    14281 atcccgccaa aaattttgta tggtatagtc caaaaagagt gggagaaact gcagaaggat
    14341 cagaaataca tcagagctct ttacacaggc ggtacctttg catatgaaac ccaggttgta
    14401 ttgactggaa tcataggtgg tcgtggtgat gtctactcaa acgcaccgat tgcaggcgcg
    14461 atgctgttgc atgacccatt taagagtctc gatcactcta ttgtggatct cggtgaagag
    14521 gaatttacca agggacgagc acatcccatg attgacccaa ccataagaaa aataagacta
    14581 atcgaagagg cagcagatcc cggtgtagca gtactactcc ttgattatgt attaggtttc
    14641 ggctctcatc cggatccaat tggagcaatt ctcagccagg tgagtgaagc caaaaaaata
    14701 gctgagaagg gcggcaggta tctagctatc gtaacccacg tgtgtggaac agctcaagat
    14761 ccacaaggat acgaacaaag tattcaaaga ttgaaagaca cgggtgcact aatcatgccc
    14821 actaatgctc tagcggctat tgcatccgca accgtagctc tgagagggaa tatagacctg
    14881 gataaagtct attcaaagta tttgacagga gatggaagtg gggcaagaag agggggacag
    14941 gtgaatccat tataactaaa gaggcaagta aaaaaagaaa tttgttggac acagaattaa
    15001 aggttgttaa tttgggtatc gacattttct acaatgcatt gaagctccaa gatgtcaagg
    15061 tagttcaggt tgattggaaa cccagccaga aactggacaa ggaatcggaa gaaatactct
    15121 caaaaatctt gtaagaaata aaaaatataa tactcacatg catcaaaatc gagtatcaaa
    15181 ccggaatcaa aaccagaacc agcatttcag ccacaaagaa atcggtaaat acgtatttaa
    15241 ttgccaatgg gtggggaatt tacacgctag acgaatactt tcagaacgat gtggtttgta
    15301 cggtgatgtg atcagcgttt tcgatactac actgaatgtg aagacaaaca atgatgatct
    15361 cttggttgtt actattagta aagtccgttc cccaataaac atgaatgtcc tgcccatctt
    15421 caaagaaaaa gaacgaggag gtggaataca agggtttaga ggcattatag attttgggac
    15481 tgaagtaaaa atccaaaaga atgagatgtc aattgggaat gtaatttttc ggctaggtaa
    15541 atgtagagtt ttccaaagtg ctttagatag gcttccgagt gcaaaccatc tatcagaatt
    15601 tatgagcaaa tctgatgaaa ttttta
//
LOCUS       DAOR01000318           26139 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_4912, whole genome shotgun sequence.
ACCESSION   DAOR01000318 DAOR01000000
VERSION     DAOR01000318.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 26139)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26139)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26139
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_4912"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5514..5818
                     /estimated_length=305
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7020..7237
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7498..7725
                     /estimated_length=228
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20436..20615
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7579 a         4924 c         4679 g         8026 t
ORIGIN      
        1 atctatgtaa gtactacaac taatcaagtg ttttagggtt atttcattct aagatggtga
       61 tcttgacttt ttttccaatc cagtaacctg gtagataaat cctgccaagg ttgtcagaat
      121 tcttgccaac gcgtttgacg agtttggtca gcaccttccg atcctgtcca accatggttc
      181 ttcgtgtttt tctaggtatc cttctcatgg tttttttcat tattctctac ccttgatcaa
      241 ttcgtttatt atgttgtcat ggtcatctct tcccatgtgt tttactcttg tctttgattc
      301 aagtaatatg gtaatcatag ccatacatgt ctactgtaca tcatgcatat ttatacaggt
      361 tcgctatgca tagaggacct gcatacgagt tctgttgctc gtacggcact tgtaaatccg
      421 tactctacag atatggatta cataggacgg ctatgttatt tgatcgagaa attggagaga
      481 aagaccagat cacatgtcaa tggtcttgta aaaatcaaac aaaccgtata aaagcttaca
      541 aacgacagtc atgtactgta gtgcattatt ccgtaaaatg gcggatgaat actaattctt
      601 atttttaaca gaacgaatgt atgtccgtct tttatatcga cgttgattct gatagtatcg
      661 gcaatgacgt tattgaatga ggtatcactg atattttctt aatatagggt atttttgagc
      721 ggatggccat ctcaacttcg ctcgtcacta attctacatc agccaatctc ttgttcccat
      781 caacttctat atggacagca gcaaatacta tcatcattcc agcaggacgc agcaaaattg
      841 atctaacctt gacctgatcc ttctttttga atttatcttc aatggcttgt cctatcaagt
      901 cagtaacctt tggattctgc catgaatcta caagaatcaa tgatgattgc ttaagtgaca
      961 catatgcaac tgagaagatg tatcccgcaa taattatacc accgattgca tccatctgtg
     1021 aaaatccaag ttgtgtagca atcaatacac tcaggaaagc taccactgaa gctgaaccat
     1081 cttttattga gttcttagca tctgttctta atgatagaag attatgccta ttggcaatat
     1141 tcctcatttg tattgccctg tatagggata ttacacttgc agctgctaag actaccattg
     1201 tcaaaatagg ctgatgaatt tcgtgtctat gttgtagtag tagtgaccca catgactggt
     1261 ataaaataat tgatccaatt acaatcattc ctattgctgc tattagtgcg gcaaaactct
     1321 ctaccttgta gtaaccaaag tgaaatttcc tatcagcagg acgacctgcg attacaagac
     1381 ccataaacac tataaatgaa atcattgtat cagagagtga atctatgcca tcggcagttg
     1441 caaccacgct tccgcttatt tggccaacca agagctcaac tattcctata ctagctaaag
     1501 tgatgacaga aatttttgca atgttctgag cacactttat gccttcactt actggcatag
     1561 gagaagattc tgcagatttt tcttgcaatt taacagctgt cagactacca ccctcagtgg
     1621 tatctacagc gtaacgttcg tccattaatt actataacaa gtactatacc aatttatcat
     1681 atttaaacgt ggagcccgtc atcatgcatc gtgccctcac ccggtatcca aaaatttcaa
     1741 aaaatggtga tcataagatg gaattcatta ataaacccgc taaaagtata acattaggta
     1801 ggctatgaac aaactaagta aaatgacttg aataggaatc tgcattcgtc gttaggctca
     1861 gtctggatag cccgctactc tattggacag aaacgttgtg ttcataaagt gggatggact
     1921 atggtgtcca tgttgtggtt atagattaac aacaaaacca tgtaacttaa aatataaagc
     1981 caaaccgaaa gcaaagaata ttaattattg ttacagcaac gaccagctcg ggtaggaatt
     2041 aatgcgtttt atttaatgac ttgtaagcct agagttctat actttggtgt ttggttttac
     2101 aggaatcaga ttttgtccaa ataggcccgc taccttgtta tttggaatga gttaatcatt
     2161 ttttgtatag tgggtagaaa ggtggggtac tttgctgctt ccgcattaaa tgaaatatta
     2221 tatactttgc cacctattat tgtccagatc aacatatcct ggaatttgaa tggtatatta
     2281 ctaggcatag acaaatacac tactttataa cctggatgtc ctgcaagaag ggttggattt
     2341 gattctataa tttgtacacc tggtatattc tttagtgtac tattaaggaa tttattatat
     2401 tggtcgagtg ttatattttg tgaataatca actcttgata atgtcaggtg ttcagggact
     2461 ctgtctgcaa ggttttctaa tggcgcgtag aatgcaacta tgttagtata atccctcatt
     2521 ccattttctg ataccctcca atctgaagga tactgaattt ttatgccata agcaggattc
     2581 tggtacgtta acagattatt gctattattt ttagagtctg tctgtgctga ggattgtatg
     2641 acagagagta ctgacgatga taacaacaat gaacttaatg tagacaataa tccaaataca
     2701 gttagtagtc tcctttggga atgcatacta aatagcctat acataacgca agtattaaga
     2761 ttaccgatac agatcgtttg agtatatact ttgtttattt atgacgtgct caagtatccc
     2821 gtttgaagca gtatggaaca aactcaagat ataagagata tgtaagaatt ggtagaatga
     2881 ccaaatcata gaagacatca acttgttgat ggacaccgag gtgaagattg ggatacgatt
     2941 attatttctg taaatttatc tcatctacaa taaatgtatg aattcctact aatagtacca
     3001 ttagctacaa cgtctctgat tatgataaat attttgatcc ttgcagccgg attattgtca
     3061 gactcgttcc aaatcacacg tgattatggc tttattcctg ataagcaatt tcatattaat
     3121 agtattgaca ggagttttgg gtgtagcagc tgctgctgga aatactagag ctgattatac
     3181 ttcaaattgt atttgctgac tttggctcta tagctgcaat tccaatagat ttcactgtac
     3241 acgttgatgg gtgcattgca ggaatatttt gtactaaatt attcatagga attgaaccta
     3301 agaaacgagc aaaatacttg atgccttcgg gaattataat catgatacgt ggcggacaga
     3361 ataattaacg cgagggcctc aaactttcta cactgacgtg ttttgctaat tggtttgggt
     3421 tcgaaaagtg tttttattca ttgcatgtcg tagtagagag ttttcgccat aaaccagatc
     3481 gatatcacca ccgcctgagg tggtgcgtgc aatgggtgta acatgcatcc gccactgcgt
     3541 aagtgtacgt ggtgatatct aaaaaagatg atatgtttgc atcaccagtc aggcgcaatc
     3601 agcagcgatt atcataaatt attctcgagt attcagccca agtcataata gactattttg
     3661 caagagcaat atcaatacca ttttgcttaa gttgcctttg aacatttttt agtatatttt
     3721 gtgtaaggtt gtcagaaccg ttgttattaa taatagactg attactttta gatgtcattg
     3781 ttgatgtaga tgtagatgat aaggatgacg atgaggatgt cttggaagta gaaatatagc
     3841 caggtgcaga tgcctgtaca gtggtcttaa aattgatagt gctactattt tttgagtttg
     3901 gttttatttc cgcagagtac gtaatctctc cgctgtgacc tacgatttct ttagatgtac
     3961 cgttagaagt aaagacaatc atcagtttta caattgcgtt attgattttc ttgccagtat
     4021 tcgcatcata agctatagca gttatagtgg atatacctct accgttaaca atattttgtg
     4081 ctgactgaat tgaaatcgat aacggtttgg cgttactatt attataatta tacttagatg
     4141 tagatggaga ttgtgtcaac ttagcagata gacctccccc agatgtagta attttgttac
     4201 tattattttc tttttttaag ctttgtgaag tagaattgct tgtgatatta ctatgggtgg
     4261 ctggcgttat tgctgtacta tttgggttca tagtgatatt acctccggtt ttggattgtg
     4321 gtggttgtga ccgatctaaa gtcgaatttg ttatcgtctg gttattcctg gtaaccgttg
     4381 atgatagtgg tgtcgatact cctgttacat ttacactaaa ccatttaatc aagttattag
     4441 tggtgttaat aacattggct gagttagaat taatattact cgcctgtaaa cacgataatc
     4501 tacctgttag cttattttgc ccctctttaa tggctccaaa tttggttgaa agagtataat
     4561 accacttaga atagtcattg actcccttcg gtccccctgc tgttgcattt tcatatggtt
     4621 ttatattatt tgcaatgacc gaaacataac agcgattatc atccttattg ttaccagtac
     4681 tagggctagt agtgttagtg ccggtcgcac tccctgaaat tgtaagagta ctgccagttg
     4741 aaatgttctg accgttggtc ggtgatgtaa tccttatgct gggtatttct gtttgacctt
     4801 ctgaaaagat cactatactg gaccatccaa tgaaaacagt aactataact ataactacaa
     4861 tagacagaaa agataatgac gagagcatag actttgattt catgaatcga tcacttttga
     4921 tatgccatca aatataaatt cgcctatttt ggtgtagttt cctaattcat gttatcgaac
     4981 atatgactca atattttttc cttcagatgg taaatccact ggttcaaatt tcctgaaaat
     5041 gatgcttgat ttgaattctg catcgagtac tgcgtaacta atacgctcta taattatctc
     5101 cgaggatttt tgaagccatc ttcagctcta gcacctcgtt cgttccttca tatatcgtaa
     5161 ttgctcgcga atctaaaaaa tgtcttgcta ctctattgct ctttctataa gcatatgatc
     5221 caaaaatttg gaccgaccta ttagctgaat caaatgcaga attgctcgca tgaaatttcg
     5281 ctatagtaga taagttatct gcatcacttc tcaataaata ataatcctta ttatttcggt
     5341 gccattggtg atcctcagtt tttaactgtt ctacgtaatc gtgcagattt tgcctagcca
     5401 atgcggcgcg gtagaccagc cagcgtgaac tttcaatgtt gactgcaatt ctagctaaat
     5461 gttcctgtat taactgcttt ttcgctagag gcgaaccatg ctgctctcgt gccnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     5821 agcggcaacg ctaagtttac catctatcaa tgcactatat gcaatattca gaccttggcc
     5881 ttttaatcca agcaagttct ttttcggaac aacgcaattg tcaaaataaa tctctgcatt
     5941 tttgatggtg agtaagccta acttatttat catttcctcg gttgaaatac catcagaatc
     6001 tccatcgact ataaacgcag atatttttcc aggatgactt ccatcggctt cttttgcgta
     6061 agttgtcact acctttgcgc atgtcccatt cccaatccag tgtttttttc ccttcagcag
     6121 aaagtaatta cctctatctt caaacttcat aaccatagac gaaggatcgc tcccagccat
     6181 tggttcggtc aaggcaaaag ccattatact cctgccatca caagtttttg gcagatattc
     6241 actcttttgg tcttcatctg cccaaccctg aagaaccatt tgacctattg aggtatgtgc
     6301 tgaaaagaat gttcgcaaag aattgccctc ttcacctatt ctttcaatag cgaggatgta
     6361 tgttaggatg tcgtatccca agcccccata acttcttgat ataggacagc caagcattcc
     6421 gattttaccg aattctggaa ttattttttc gttcaacctt tcttcaagat agcatttttc
     6481 ttcgtactgg cgaattgatc tacagatatc gtcaacttct tttagaaacg ttttctgttt
     6541 atcagaaata acgaaactca tttgcgataa atttgaaaat gacacagggt ttactaatat
     6601 caattaatgc aagatatgca tatttattag cagctttaat cagttctctt gtcaaaatac
     6661 taaagacata gaatgttgga atttgattaa ttctgtctaa agtaggtaag tggctttgat
     6721 tccgcaggaa ttgggtgtga tcttttttgg ctggaagaga taaacctcca atattggaat
     6781 gtctaatcat acaacatgaa gaacaatctc gtttttgagg aggtaaaaaa aaccgttgaa
     6841 gctatacgat atttccccaa cgtggggcta cacgcctgga aacaggacaa caattaccaa
     6901 ccgctctatc tcctggatgg ctgaaatggt attttgaact caagcgtgca tcagggtgtg
     6961 ttgtaggaga agcacataga tcttcttcat cttacatgta tgactgcaga gaatgtgacn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa cgtgcggtca tatgcaaaac
     7261 tataagaaaa caaaccgaca tttgtcaggc actggaataa agagcgcagt tagaataaat
     7321 tgaaggtcca aatcgagtct aaaattatcg catatattat acattaataa agtacgtata
     7381 gataccacgt tcgctgtaac atattataaa aactaactaa ccctcatcct gaacaagatt
     7441 ttaaataccc catgcggttt ctaatatgta gacagcatat atctctgtat gcagaatnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcata tatctctgta
     7741 tgcagaatat actctccaat aggaatgtaa ttttatatca ctgttataaa aacggcatag
     7801 tgtttaaata gtttcagtat agatcgaaga taagtgaatt agatgccagt cgcaataatt
     7861 ccggatatta gcgaacaaca ctgtattggc tgtgcgctct gtgttgagat ttgtacagct
     7921 ctcggtccag atgtactgag agtaaagccc gtggaaggat ggaagagagg taaagcattt
     7981 gtcttttacc cagaaagatg catctctgac ggagcatgca ttggtgtttg cccaacaaag
     8041 gcaatcttct ggatgagacc gatggactat acgccaggcc agccaattcc attgaagaag
     8101 aacgcagtgt tcacaaaggg ctgggaagaa ggataacttt tctcctgcag tccgaaaaaa
     8161 ttcccttttt ttaactctag actttgtatt gtactataaa gtagattgct acttaattca
     8221 ggatattaat atcttaagta tagcatgatg cgttactcga atatctctta ttcaagatct
     8281 ataagacata gattgtacta gaataatcgt ctattagtaa tgtaatatca ctaacgatat
     8341 gcttcaagca ccgtttcagt cagaaatcga ccagaccaat aaatagtagt cagaatttgc
     8401 acccgatgat tttatcctgt ccctgcatac acactaaata cgttcagact tttataattc
     8461 cctacccata tttgtcataa cgaccttgac tttgaatgga cagggaccgt catctaagag
     8521 tggtcaagaa aatacgatct agtagttatg ctagagaaga cttcgacgca gcgatgaggc
     8581 cctataatga attaaagaac gcaggtctaa acgcatggtt agacaaatac aaaattcttc
     8641 caggtcagaa ctggggatat gagattcaag atgccataga acacagcagg gatattattc
     8701 ccttgttttc ctctacgtct gtattacctt tatgtgttca atgattccac tctacacctc
     8761 tcgattacag actagtcata cgtttaaacc attgtctcct acttgtattc ccgtgcgcct
     8821 tttctgctga tttccagaat gtctaatcat attatacctg cttcgactgg ctttcgagca
     8881 ggtgatgctc ttttgaagcc tctaatacca ccgccaccat tactcctgcc tctgctgtac
     8941 ctcaattgta gacctaattg gttttcatta ccataatgca aaattcaatt attctcaact
     9001 ggtaaagtca acaagagcaa atataactac aatagaagcg tgactagaaa gttcttatag
     9061 gtcccagggg tacatttaca aatggaggac atatactatt actactccag cagcaatttt
     9121 ataacagctt gtaagatgag gacttagaga tgcagaaaaa ttttatacaa tttagattgt
     9181 tcgacgagaa gactagttcg aagcctaagt tttgtgtaac ttgtggcctt aatgccactc
     9241 aggaggcttt gttcaatgtg ggcgatgggg taattttagt agaaaaatat tgtgatgcat
     9301 gtgcaaagaa cgtgaagtag acaaaaaatg acataattcg ctcaatatct taactactat
     9361 ctgacttttt gttgttgtat tacttctatt aaagacgagc tggatgcaca ctctaaagac
     9421 caatagaact cttttggatt atctctagct tttagacctg catgcgcaaa atatattgtc
     9481 ggatccagtt taaacccgtt tacggcttac tctcatatcg ttgtgtaatt gaagttatta
     9541 ctaaagaacc cttactaaat gcatgctcaa ttgtccctgt tatgtgcaaa tatgttctcg
     9601 atgagaagct catgcatagg tggctgcctg cataagcata ctgagggtag tctagatttt
     9661 tctatatctt ccttgtccgc aattcagctt tatatttcaa gttacatggt gtaattctca
     9721 atctacaacc acagcatgga caccataatc catccctctt caagaaaatt tcacatatct
     9781 cacaatgttt ttgtcctata gaataacggc cactgccagt ttgtttcaat gctttatgac
     9841 gaacccaaaa aataccttca accgccgaca cctaccgcca acaataattc aaatctagaa
     9901 ggaggactcc gacgattatt ttcaaaacac caaaatcact agctaattac aagcaaatgg
     9961 aacagacaat atgtgatcta gcacttccag ccaggaaatg tgaaaaatta tccgatccgc
    10021 agaaatcgaa taatttctca tattcaaata ataatgtctc cgaaacgtct tcgaaatcga
    10081 agtctggtga gaatgttgtc cccgaatgcg cagcctcaaa taagacattc aaatgccttt
    10141 actgctgcga gtattatcca agcgatgaag agcgtgtcaa acacatagaa tatgaacatc
    10201 caggcaaaat gtattgtcct acgccggaag attttcgtaa ccgacgactc atcaatgaat
    10261 attttacttc attgcaaagc atagatagtt ggcgatatta ttactctaag cgtttgaaag
    10321 tcctgaaaat ttcacgggac ccggagtctg attttgcaat caatagtagt ccaaaaatct
    10381 tatgattttg ccacatctag tacattttag cgcggcctgt aagattgttg ccgcagattc
    10441 acaccgggcc ctgttcaaat attcgtacac atttagtatt tcatcttctg ttcccgttgg
    10501 tgctacagcc gccaccaccg aagccgcccc taacattact ggatccgcca ccaccaccag
    10561 ctgcagatgc tgttcctaca acgctgtttg gcacgattcc tattagtgct gctgcactta
    10621 aaacagtaac aatagcaaat attctctcga aatacatgat caaagcagta aatatacaac
    10681 tatttatctt ttatctggag gttctttaat ctcttatcat ttgatttcta tatgttcttg
    10741 tcaattcatt aaggaagcct tcatacttgt ccaaatcaga tccaaaagat tttgaattaa
    10801 tagtataatc atatttttca aaaaatttgc acagcttaaa tatctggtca tcgcttatac
    10861 cataataatt ttgtaaccta aacaatgtca ttactgcgtt attttggcgc gcagaaattt
    10921 ctttcatggt aaatactcgc gttcttgtgt tatacaattc ctttttcata ccacccaatt
    10981 tgttatagtc ttggacatcc tcgatcacac ggtaatctcc attgacaaat tatacatttc
    11041 agccttttcg ctaacagctg tatgaaacgc gaacagctta tatagtccaa ttcgaagccg
    11101 aataatttgt tcagctaatg gtaatacact ttgacatcta gcctttctca attatcttgc
    11161 aatgctctct cttcgtttta acgatttgat tttagagaat tcctcgatta ttttcctagg
    11221 atcgtctcga tattgtttga tgttattaag tagtgttaaa agcctgttag tgtcttcaga
    11281 ggagagatta tgtttactta attccgcctt gaattgcttg taccggttta cggtttgtat
    11341 ttccacattt gtacgttcta aaatcgcgcg tgcttgttga tttcttcttg aagtctttgc
    11401 tcttcttccc ctttagtttt acatggcccc tacaagtctg caagtggtat tgattcagag
    11461 attgatacac acgcgacctg atttgcgacg tcgatcagcc tttcgggttc gggtgaattg
    11521 accaaattga ctaggaacga ttcgattttg tcttcattcg tgcccatata tttagtataa
    11581 ttatttagtc tgaaagagca agcaagctca cttaagccaa ttcctcgttt tttgagagag
    11641 attgttaatt ctcttacatt atcatattct gaattctcga ctcctttttt ccattatcgc
    11701 tagtgaaata ttggattcat caataataat acatattgag ttgtgatatg tatatacttc
    11761 aattctttta ttgttgccat cgttaccatt gttgttttct atacccccct ttgaaggtca
    11821 gagtaatatc aggatcatct tctataatca aaattctctt cgacggctgt ctcacctttg
    11881 cggtaacggt gccatacact gaccttgttc aaaaaatcgg gtagtcattc aaacggtcct
    11941 tttactaaca ccatactatt tactaccgag ttgtattgtc acattattga taatttcgtc
    12001 tatacgttta atcagatctt cattgccaat aggctttcga atgaaatagt tagcaggtaa
    12061 agagaaaata tatctgagta tactccataa tacatttctc ctgctgtaag aaaacagatc
    12121 ctaagattct tgtctaattt cttaatttcc ctgtaaaatg aaaagccatt catttcgggc
    12181 atcttaatat caagaattgc tagatcgtaa taatgaggcg tgaaattctc taaagccatg
    12241 agaggacact catacgaatc aactttgaat ccattttgtt ctaacgcttt ctctaatgtt
    12301 gtgttaatgt cgggttcatc atcaactaca agaatcctca ttgacgtctt attgactaat
    12361 agcctccctt tgcttctgtt ctctgcatcc ccttccttct tcttcatctt tctaagtgct
    12421 cgctagagca agctttacct ccttgtacat acaagggctt tatcgtcttg tcgtgtatct
    12481 tgcaagctgt aaagtgccag actgcttcaa gtcctactag agttactaca aacccgattg
    12541 caggatagat cagatattca aacataccaa acagtgtctg gcttcttatt ttactttgat
    12601 aagtaactct tttgataaat cataaagttc gtttagtgcg gtggcaaagc aataacccct
    12661 ggtatcaacg agttcctgat tttagtcgtg tctattaata tgaggcataa aagaagaaga
    12721 accgttcatt tgggagttat catcacgtac tctgtcagtg cgagcatctc caatcacatt
    12781 attgccagtt gtctagacgc taattgttat gtgcggatat catactccaa actatgcttt
    12841 atattactaa taacagaaat tacaataaat ctttatgagg tttaatgctt cgtctgtgac
    12901 attatttctg gtagtaatgt taaccctgtt gatgccttca gcagtattat tggccttcgg
    12961 ccaaaactca caacctacaa caaatttcgc ccaaagtaaa tatgaagtaa taaagacgat
    13021 tcgttcgctc cgattcgatt ttccatatac agcatcaaac aatgatagaa cgcatccact
    13081 tgtttatact tttgtcaaac ctatatctga gagctggatt ctaaatgtag acaataatgt
    13141 atcttatgcg actagacccg atgccaagac agttataaag ttgcaggaac ctgcacccag
    13201 cgagaagttc attgaaattg caatgtatgg ggatagtgcg tctaataggt tttgggcagc
    13261 tgtaaatacg agagaagccg gatatatcag actatatgat aaaaacggta ttgatggctg
    13321 gtcaggatca cagccaataa ttattggata cgatagcaat caagggttat caatcagtat
    13381 tggcgcaaag gttctggtgg atagattatc tgtaaatggt ttcacacttg gttcaattgc
    13441 ggtttatgga aatgatgacc caaattctcc tcccaataca tattctggca atatatcgtt
    13501 tgaggtgctc tggggaaagc ctgctgattc tccaatatat tatctgccac ttgttatgct
    13561 agttggagtc ggtggtgttt taattgggtt acttgtattt aagaaaaggg acaagattga
    13621 agctagttga aaagaataac agctttcagt tactttcggt aacatatata atcagacttt
    13681 tcggaaatag ccgtaataag tcaatagcta cttcgatcga cactatggaa ttgatcatgg
    13741 taaataggtg gtggaaatac gtcgagcccc atcagactgg atctgtgtga attcatttac
    13801 gatttgggtt gctatactag gaagagtgta gaaacccctc tgaatttttt atactcagat
    13861 cttataattt gtagtatact ttcaaatatc atacaagcga ttaataaatc taaaatggat
    13921 tatacttaca tccccctttc ttccgtcttt tagggcagca ctagctggtt tgaattgggt
    13981 ttccttcttc cagcaagcaa caactagact tgcatctatt cggcggtctt aatgattctt
    14041 ttcaacacat acggaatctc actcaagcta gcgattgtat aagttggacg ttcaagtccg
    14101 ctcattggtt cttgtaattt gaaaagtgag tttgtggtcc tgattgtttt cattcccagt
    14161 gcatttgctg gctggatatc tgtgtctagt ctatcaccaa ccataataca ttcttttgga
    14221 caattcaccg cggcaaggtc caatgctaag tggaatattc tgggatcagg cttagcaatt
    14281 ccaacctccg atgaaatgac cttcaccttg aaaaatcgac ttatgttaga atcctccaat
    14341 aaccttacaa tatctttagt ttgattagat attattccaa gatcaagctg cctcgaaaga
    14401 tcgttgaggg tttcttctgc gtcatcaaaa aggcggagga attttcttcg tccctcttca
    14461 atttgatttt ctaatctttt tgtaataatt ttgccgtaac caggttgaga tattaatttg
    14521 caaacttcgg ttactaactt cctaacactt tcatgaccta tttggcggtt tctaataaca
    14581 ctgtctctta ctgcccgata atttcgacta tcgattcttg cgccaaacgc atttaatatc
    14641 tctaagaatt ccttatcaaa gtaattaata aaatcccact cgtttactaa agtctgcccg
    14701 agatcaaaga acatgaatgg tcttttcata caaattcgtg cttgtggcgc attggaatct
    14761 gattaaatta attaaattaa tgtctatctg tacctcaaat ttcgggggaa aataacacag
    14821 tcactggatg caaagtttga ggtgataatc attgtcattt accgttgacg ctctggttcg
    14881 ctggtaattc aagcgcagcc atcgatcctc caactccatt gtacccttga actttacctg
    14941 taggaagaaa gagcaccatc tcccagagat ggtccgccca ccattattgg accggcaaca
    15001 ttaaacttcc atggttcttg tccagtatcc ttatccagtg ccaaaattgg tcctgatctt
    15061 gtttgatgtg tcattcttgg ctgaacaaat tgacaggctt ttccatgata ccgatgtaac
    15121 cggggaatac tactccatta gtaaccaatg gtgatactag tggaggaaac tgttgcgctg
    15181 gtagtatttt atagataaaa ataatataat aaagttcttt caacatgttt ttattgatgc
    15241 aagttcgcaa ccaattattg ggtaaaggca agataattgt aattgaggga gccgataaag
    15301 ctggaaagct gactcagtct agattattaa tcgattcttt aaaactatct ggtcgaatat
    15361 gtgttgttat ggattttccg gattatacta cgccaattgg gatggaaata agggcctttt
    15421 tagacggtaa acgcgagtat tccaatgaat taaaacatat gcttttgtca gccaaccggt
    15481 gggaaaggaa aagagaaata gaaagtatga cagagacggg cacgatagtg gtaataaaca
    15541 gatactacca atcaaatctt gtttatggcg tctcaaatgg tcttaatatt aactggctaa
    15601 ctaacctgga acggggttta cccaaagaag atatagttat agtcctagac gttagtccta
    15661 ccgtatcgtc ccaacggtca gaagcgggag atctggactc gtttgaaaaa aatcagaaat
    15721 tgcttttaga agtaaataag aattatcgta aacttgcaaa acagttcaaa tggaaaataa
    15781 tcaatggtga aaaaaggaag gagcaagtcc atcaagaaat aatgaagatc gttaaactcc
    15841 tgaatgtcat ctgattatca cgctgatcga gtattaattc gactaaaaac aaatcatttc
    15901 agttgttttc gtttgctaaa aaacaatcgt taatgactcc cttacgagac ctcgagcacc
    15961 gctcttcaag cttagatgat cagctacaag agaaatgata ataaactcga cgcatataag
    16021 ctgattggaa ctcactataa acaatagtga actcgtcgcg ttcaaggata tgacacttta
    16081 cagggcgagc cagaaagata ataatagtca gtatcttgac atacagtaga tttaattttc
    16141 tgccttacaa acacaaccat cagatcgagc tatgaagttt gccggagaaa tcaccgatcc
    16201 agttcatcaa tatatccgtt tcaccaatgt cgaaagagaa cttttggaca ctgccgcgtt
    16261 ccaacgtttg aggagaataa agcaactctc tggcgcacac ttggtttatc ccagcgcgca
    16321 gcactctcga ttcgaacact cacttgggac gatgcatgtt gcaggcttcg caggggaaac
    16381 tcttcttgcc aagggatatc tcagtaaaga ggaaagtatt caagagttac gattggcagc
    16441 tttgcttcat gatataggac atggaccatt ttcacaccta tttgaggaaa tgttaggcgc
    16501 aaaatgcgac ctgacccacg aggacttggg gaagaaaatt atcctcaaga cagagattag
    16561 cgatattctg aaaaagcacg gttacaattc aaatggtata tgtaagttat catttggtga
    16621 atcaaagata aaattcttta acgaaattat cgccggcgca ctatcggcag atattatgga
    16681 ttatttacct cgagatggat tgtttactgg agctgaatat ggtaaaatcg actatcacag
    16741 actaataagt tcgtttgaag tggtctcgaa tgcccatcta gccattaata gatccgccct
    16801 ctactcattt gaatcaatgt tgatctctag gtacgaaatg ttcaaggcag tctatttcca
    16861 taaaaccgtc agatcggccg aagtcatgct tcttcattca atgaggctag cagatgaaca
    16921 gctgaattta actgacacat ccctagataa ttatctaaac ctgactgatg attcaactct
    16981 tgagaagata cactctctta ataagagcaa tgaggttgca gtcagactag taacagatta
    17041 caggaacaga aaattgctaa aatgtgttta tgaaaaattc ttacacaagc acgatgatat
    17101 ctgtagaaaa atggatagac gggcactaca taatctggca tcacagatag cagaaggcgc
    17161 aagggtccaa gaaaattccg tttttgtcga tgcatcgaga gcatcatcta tgccgctgac
    17221 gcccactaaa aaagagataa gttcgatatt gctcgtcgac aaggaacggg catatgaaac
    17281 gcccatctca gagattccgc ttattgattc tgtaacaggc tttcttgata tgctaagagt
    17341 atatacaacc ctcaaaaata gagaagtggt ggaacgatct gttaaaaagg ttcttggcga
    17401 agaagattcc atgtaccaaa agcacagaat caggaaaagg taagtttgac atgaataata
    17461 tcaatctgaa taaaaaaaga agaatagtca taaaattaag tggcagcata ttcaatactg
    17521 ctaacggtaa ggagtctatc acaaaatatg ctaagatgtt attagaaatc agtgacaaat
    17581 ttcaaccagt agtcgtgaca ggagggggag aaattgcccg ccactacata aatatcgcaa
    17641 ggagtctcgg gtcagatgaa gcaaatctgg atattatagg gattgaagta tcacgtttga
    17701 acgcaaagct gttgcttgtt acattaggag atcaggcctt ttcccaagtt ccaaaaaatc
    17761 ttgaagaagt agcattagca gcagcaagtg gtaaaatagt aattgcaggc ggtttgcatc
    17821 ctggtcaaag tacaaatgcg acttcagcac ttatagccga aacagtaaag gcttcaaagt
    17881 ttctaaatgc taccgacgtg gatgggattt atgattcaga tcccaaaata aacagagatg
    17941 caaaattatt taaaaagatt acagtcaaag aatgtatgga aattctagga aatgaaagtt
    18001 caatggcagg aacgtatgaa ctaatcgata ttgttgccct taaagtgata gaacgatcaa
    18061 gaatcccgac acaagttttg cgatcaaacg tcaacaatat caaacatgca atcaataata
    18121 ataacaaata ccatgtcggg actgagatag taattgaccc gacgttgacg agagccgcaa
    18181 cggtcaagaa acggtcttaa tcatacccat aaattcttga atgccgtctg ttatcatctt
    18241 tctatatatt tcctcacttt tttgtatagg caaaggtttt gattgtgcct ttgcataccc
    18301 atctttatca cagataaata acattgcctc atctcccctc gctattttgc cagcaagcat
    18361 ttcctcacct actggctcaa aacctttgtc gctttgcttg atagtatacg ttagcatcgc
    18421 gaatcctgca taatcaaata atttcatctg agcttttttt gccctttctt gcccaaggaa
    18481 agcagcctta tgatattgca tatatcaaat gaattttatt ttgccattat ctataagctt
    18541 tcctttccgg gatacaaatg ttttcgtgtt cgatattcac cgataatcag taacgttgta
    18601 ttgacacgcg tggttttttg agcgtcgttt gcaacattag ttagaaaaaa gtgctgactt
    18661 gttgccattt tccaactgct ggattaacct tggaagggac gtaatacttg cggaaagtac
    18721 gtgtttagaa ctagctaaac caaacgcaat tgcaaaatta accttagaag gttaatggca
    18781 tttgaatatc aatcaacaca gcggctttgc taccatctgc tattagtcca gagccaacta
    18841 aaccacttgt gcatggctag taaatgatgg aagtgcaaag tacaagtaac taaattccaa
    18901 ataattgctc ctgacaatcc agcaagatga cgggtggcat aattcctttt ccgatacaat
    18961 acaaaaggct caataaagcc gtgagtgtat tttcaaggtt ttcaagttag gagtatgcaa
    19021 gagctttgaa taattggttg aaatctgcct tggctgtttc aacaaatacc caagtattgc
    19081 cgccaccgcc aatgattaca gtccagtcgc caccagaata tgaccatacc ttttgtggca
    19141 cccatgtggt cttagtaaag cgcgtaaacg ctccagccaa tgtattaaag agcattgaaa
    19201 cggtagcgca gaattgagca gatacttcag caatttcttt cgctatatcg gtgtttgatc
    19261 tatattcaat gatttttcca tctgatgcga attcaccagc cgcagctacg ccatcaagtg
    19321 ttaacaattc atcgaaatta accatcgcct ttagaaaaag aaagtcttat attaaaggtg
    19381 cgaaataaaa gtatgaagga tttttttacg gctacacatt cacttcatgc atcccccatg
    19441 agaaaagcgt accacttata gaatagcgga cgttagactt agaggccgag ggaatgtaaa
    19501 attgtctcga catgaacttg aattggagga tttattcttg aaaacctgaa agaagttgat
    19561 acgctttaca gatccgtaca atcagacatt taactaatct tagacacgat ggatgggcta
    19621 atatccggtt caaacggttt tcagcacggc ccttatgata gtactttgtt gtaatcttac
    19681 catgtcggtt taactggttt ctcaacagca acgacaaaga ctattcggct ttcttcgtcc
    19741 tttttttggt tctctgtgtg cgggagaaaa gacagccaca taagaaaacg tcgagttcaa
    19801 tcgtaatata atactatcca gagatctggt agttaattga tatctctgga aaaatagaca
    19861 cataaatatt tgcctttagt gccagttagt ggagaaatcg caatcttatt atcatctgca
    19921 atgtgcatta attcgaattg catttttgtt gagttgtctt gcagatgaac gtcagcagta
    19981 ataattcctt tttttatagt aggaattaga taattcaagt aaaatagatt gctaactggg
    20041 taatgagtat ttacgaaagg atcctttctt ccagatatca tattaccccg tctagattga
    20101 ccaacaatta gtttcccatc atcgtcaact acagcagcaa aactgatatc tgcattagat
    20161 gataatgcaa tgaagcatat tttgcgtaaa tagtcccgtc ctttacccat taaatgtttt
    20221 cattcaattc gccttcataa tcaatgtgct agaatattat agaactgagt ctttgatgga
    20281 taatcaaaaa atttctctag ataaatccaa ataatatatg ttgtagcact atcaacacta
    20341 ttattccctc ctcatctccc atttccacca ttcccgccgt ttgcattacc tccattgcca
    20401 ccctttccgc caactccatt agcaatgcct ccattnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgcc tccattggcg gatcctccga
    20641 gacctcctcg ctcaccattt ccaccaaaag atgaaccatt aacgccattt tggccattct
    20701 gaccgttcac accatgagtt gctcctatcc ctgtgacgtt cacgctctta aattttgtaa
    20761 aattagtaaa gcttcctata caagatactt tgaaagttat tttattttgc cttgttttaa
    20821 agctgcatat ttaggagtaa tagtatattt ccgtatagaa taatcgtttg ttcctccatg
    20881 tcctgttgct gctgcctttt ggtatggttt tatatcatct agaagtacta aaactgtaca
    20941 atcagtatat gtcttatctg ctgcgggtgc aggtgaagat attcctgata cgatgatatt
    21001 gctatcaacc ggaacctgct gacctttatg aggagaaatt atttttacag agggtgtcgt
    21061 tgcagatgat gacgatatag caaatgaagc accagtaata caccatgagc aaaaaaaaat
    21121 acggctatta ttgtgaagaa gagagctacc cgtacggagt gattaaaatt ccatgttcga
    21181 gagtactttt ccatttctga tgtgtatgat attgcctata aacactattt atttaatttg
    21241 tctgctgttt gtcctcggaa aaggcttcta tctattttct gcttcttaga atcaatttct
    21301 cagattgctt agattttttc tgtagctaca tcgagtataa tctgaccaat atacgtattg
    21361 taatctaggc aacgttttaa ttacgatata gcaatttgta atttatgcaa ggagttcaaa
    21421 aattaaagtg cacctcttgt ggtgttatgt ttatgtcgga gactgagact ggcacaacga
    21481 tttgcccggg atgctctgag aatcagtcac atggtcacga agctgagaat cagtcacatg
    21541 gtcacgaaga tatgggcggt tgtggttgcg gacatagcca ctgaactttt tttactgggt
    21601 gaatccccag atcaagctgt aagaaacgcc ttcccgttct cgtaattcta taatttcgtc
    21661 atttatgtgc actatactct ggcaaatcat ttgtgagcaa aaacggtagg taatcataca
    21721 actgttttga agttatagac gcagcatacc ttatgtatac catccctttg attagctgct
    21781 tctaaaatca gggcaattat ttcggtccca ctcctgtatt ttttatatgc agattgatct
    21841 cgtgatgatg atattatctc tacatgtatg tctttgtaag agtgaaataa caatatcatc
    21901 gatggacgtg tcgatggaca cgtggatgta ttcaaatcaa gcacggtaat attcacctgc
    21961 tcatatgatc tggtcgaagt cggctgtatc cagccaatat atagtatatc acatcgtcag
    22021 atcaacgagg tgtgtacgtt gccaccgact gtaattattg ttacacatgt acagattttt
    22081 atcattacat acaataagaa ggctagacat actttcaccc atgccgcgtg aatcctgttt
    22141 atggagatac tgctaagctg ggaaaattcc tttattagtg gagccagatt acaatgaagt
    22201 tcggattttt ccatctttaa ttaagtaaat aaatcatagc ttttttggat ccttatttga
    22261 ctaagtgact atttctgcga gtttctgttg ctctatcgca tttttaactt ccatagcaat
    22321 tcttctgccc accccaaagg tatgaccgta atagtattta gtataagggc tcgttgttgc
    22381 cataataggg ttccctggaa ctctgagcga aacatcgtaa accaccagat cgagttccac
    22441 cgtaacaata ctttgtagtg agatcggtcc gattattccc ggagggtatt ctctattcgc
    22501 cgatgcagtg aagtcatcac ccattttaaa caccttttcg agtagcgatt cgcgtatact
    22561 cgcaggtgtg tgtcctattt cgatattttg taactcgaca tctatttcca tttgttgcct
    22621 ggccggtact gaagttgtga aatcattgag attagtctgc aaccttctgt caatacctaa
    22681 gaactctgtc tcccctctca ttggcgagta gaaaaagtta aagttaaagt atgtaccaat
    22741 tacgtattcc tcaatagtgg cagagtcgag atcgtcctgg ttaatcagac cttgtttgat
    22801 cttgaggttc gctttcttca tgtaatcctc gtaggaggaa accataaaaa atgctctctc
    22861 tagtttccgt tttgcttctt gaacctttac catcgctagg ccatcaattt gtgagggttt
    22921 tgtgtagatc cttgggtgcc tgatgctcgc cttttctaga agatagtatt ggttcctctc
    22981 tgcagtacgc tcctctgctc gcaagagatg tcggttcccg aatataggta tctctaagtt
    23041 attttcaata ttatcatagc caaggtaggc agtaaaggca cgatgcgcga tcattatcgt
    23101 gtttaaatgc accaattttt cttgaatttc tcgattcaac atatcagaaa acttgtcaag
    23161 taaaatgatt tcatctgtta gccttttaaa tcgttgataa gggagctctc gacccttttg
    23221 gcaaatgcaa acagttttta tgttttcatc tttagccccg tccatgattt ctagagcaga
    23281 gtgacttcct attgtgccaa tggttatgtt cttcaagtca tattcattga caatgttcgc
    23341 aacccgttgc cgagtgatca tgtcaacgtg tgtaccgacg agaacgaatt gtaataaaag
    23401 tttctaaatg aatatgcaac ccttctactt tttattcctt tatttattta tttatttgtt
    23461 tttactctta ttaatttcgg cttcaatggc aatcctcaac tcgtcatcgt tcttgttcgt
    23521 ataatcaatt ccaagagtct cggcaattgc ttctagtttt tcccttccta caacacttgt
    23581 gtcttggttt ctaatctgtt gaagctccct tcttgcctca attctagcct tctcgtattc
    23641 tgttgtcgcc ttcccaaaag acttagcaag ttcagggatc ttcttgactc caaaaatgag
    23701 aattacccca ataaggatga tgattatcac ctccacgctg ttgactatag ccatcataag
    23761 cgtcgttaat tcgaaaaaca ttatgtagat ttatattgat aacattgatg atttaagttt
    23821 aattctttat gtaatttaga agcatatgga cttggcgttc ctatactcct tctcttctcc
    23881 tttctcacaa ttgccataat tccaactatc ataggcactc caacaaacca ggtatagtaa
    23941 ttccacttcc atcgggcatt attatagcac cgaaaattac caaaataata atagcatacc
    24001 gaaagttatg tctacaaaat ttggcaccaa ttaatgcagt atagaaactg caaacacgac
    24061 aataggaact caaacgcaat tccaaagccc agaaagaatt gtagtacaaa tgatataaaa
    24121 tcattcacta ttaagaaatg tttctacgcc gatagcctgc ctgtacttgt agagaaaact
    24181 aagcgcgaat agaattactg caatatatga gaaggcaata gcagccacga acaatgagat
    24241 cggaggagaa atacacttat tattcctgct ccctttggtt gtttgtcaaa tgcaggggaa
    24301 ataaaccata tatctacctg ataattatct gtacggaggt cattatacca atcaacattg
    24361 aaacatgtag ctgagcaaga aagcttggct ggcgaaagga gcatggcctg catagatgag
    24421 gtaatttgga tggcaaaatt atgaaatatg tctaggtatg ggtaatagaa cacaaagtga
    24481 actccattaa attcaaatgg tcgaaagaga aaggttatag aaaaatggtg agaactgtaa
    24541 tcgcaattac tatgcgaggg attgtggtac gaagatcctc gatattctcg cgaaaagtca
    24601 tttctgctga cctgatgtat tagtatgaat acctagtatc gtaaggaaat ataattgtgt
    24661 gcgccgtatt gactgtggaa gtgttttacg tataaaaagt ctaggttcac agataataat
    24721 gggttatgct gggcagatga ctggagtgca attctgcgta atctaaaaaa atagcttata
    24781 gataaactat ctcgtgagta ctgtcaatga aatagcatca cgtaaggaga ttgtcacaat
    24841 aactgcagat tcgggaaaga ccgcttacga agttgcagac ctgatgaaga ggaatagagt
    24901 gggttcaata attgtgatag acaaaaaaag acaaccggag ggaattataa ctgagcggga
    24961 catggtcaaa cgtgtttgcc tcaaaaatgt cgcgtcaagc agaataaaag tagaagaaat
    25021 tatgtctacc ccacttatca cgatcatgtc ttacgactca atcgagactg cctcaagagt
    25081 aatgactaaa aacaagataa agagattaat tattttagaa gcagataata gaattacggg
    25141 cgtgttaagc gcatctgata taaccaagca cttggcagag atattgcttg atgatcataa
    25201 tcagtatagg tctttacggt ttgcggttga tctttcttag caatagcact tattgttggc
    25261 cttgcttgag tttgatgtaa ttctgaagcc tgatcctgac acaacaagct tcagaattta
    25321 acttttagta gatcgtgccg atcacttaat ccaactgatc atggtaataa gacaattcaa
    25381 gtcattgcat caccttgtct atatttgtgg aactgatgcc acgataaccc agtacactta
    25441 acgtaaacac caaatatagt aataatccta ttctcgcgat attttgaatc attttgttct
    25501 gtacgcatat aagtgtcaga aaatattagt tctttcgcca tgtccttgaa cgaatcgaat
    25561 ctgtgagtta ttaagccgaa aattatgaag cctcatcaga cgttggtata aaccaacatg
    25621 cgaaatagtg ttactcggag agtttagaga agatttcaaa tgtgctttta tttaatcttc
    25681 gacatcttta gtcaagttac tgttgtattg cttcttcaaa taacttcttt cttaccttaa
    25741 ttggtacatc gtagtaagcc gctaaggcga ttgaatctga tgcacgataa tttctcaaga
    25801 ctaaattctc ctttctactt ttaaagtata gatttgcacg tagaactgaa ccgctctgat
    25861 aaactctcac ttccatcaga agtaagtctt ggataactgc tatctcttca actagattgt
    25921 agattgtcgg tatggtattt ttatcgccgt ctcggaagcg taatatatgc ctggcaacct
    25981 cgcccgaaaa agcacgcatt ggaaattctt tcccctcaaa tgtctttaat attacaacgc
    26041 cttctagtcc ccgctcgtct gcaaaaccga catagcttat ttttgccaaa acatactctt
    26101 catctgtgtc tggaacttca tcaggcatat gtctgtctc
//
LOCUS       DAOR01000319            2648 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_74162, whole genome shotgun sequence.
ACCESSION   DAOR01000319 DAOR01000000
VERSION     DAOR01000319.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_74162"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          738 a          548 c          569 g          793 t
ORIGIN      
        1 tctgcatctt tgtctgtctg aagaaaccct tgtcggggcg taaaaaggac aataccagtg
       61 atccaacgat aatcatcttc cggccatatg tgttcatgaa tcacattcaa caggttttca
      121 gccgttatta cctgatgacc aattttgatc acataaaacg ctggttgact gtgagggaat
      181 tgatcatgct tgctcttcag tttgttatgt agtgcattga aaagttgatt attggctttt
      241 tcaatgtctt ccggtaatgg cagtgcaatg tttgcttgtg aaccaaatgc agtaccaact
      301 gtagcaccag gtgtagtata tgcggctgca agagcatgta tttctggaag ggatgattca
      361 ttctctactt caataatagg aaagggttcc caattaatta gtcctttgct acccatacgt
      421 attttgccag taggagagtc attcatacca tcaagtacct gtttgataac ctcattggtc
      481 ttaataggtt caaatttccc caactcttct gactttaagt aattacttac attgctgtta
      541 cacgttgttc taaaatctgg ttcgttgaaa agagaatcct cctgatcatc gtatattgtc
      601 aggaactagg ctgttggtgt cgtgctacga acttctcgat tttctcgact gtttttttcg
      661 tcaggcaagt aggttatatt caggccatag cgcacaactt taatcacaga cttcgtgtct
      721 ttatggatga gatttgctac gtagatagcg tactctttat aggcctgtag cggttcagtc
      781 catgatccgc cagtgtcacg gttcagctgc cagataaaaa tagaataggg gtgagtcaca
      841 ctaggctcct acaaaaaata cttctgctta tccatagaac gtatgaccca aataaaatta
      901 tcaggagctg agatgaggaa ttggtaatgg ctgcgtaaaa cgaatcaaat cccgctcctc
      961 agttattctc ccgattataa ccgtcggctt cgccgcttag actcgaaccg tgtgcgaaac
     1021 tcttgacgaa gttgagttac agagactgtg ctatctcctg taagttgata gagaaaatcc
     1081 gcgacaggcc atgtgaacaa ctccccttgc cgagccatgt aaatacccac tgactcaata
     1141 ttaagcgcgt tatcataatc aagtagcaga taacccacaa cttgtcgtaa atgcatagcg
     1201 tctatttttg accctatggt tgtcttaata tcaattagac acccatttac cataaggtca
     1261 gcgtctgccc ctcccacgtc actactcccc gcaaaggtag ggttcaggac gaaaggcagc
     1321 ggtaacagat gggagtgttt gtcgtaaaat aaataggata atgcacaaag atcatctatc
     1381 caagcatttt cagatatagc cagcagttca tctacgctct ttctaggggt aggtaacatg
     1441 agaaggctat ccaagtagcg gcttgatcgg tatacttctt caaagagtcc cagcacgaaa
     1501 cagtaccgag ctaagatccg ttcttcttcc agttccagtc gccgtccaat tggttgaata
     1561 gcttttagag tagcatcaag ggagtcaaag aacgccttga ctaacttcgg tggataaatg
     1621 ccttctgcca gttcaactat aacaggatgt ccagcttcgt catgcactgt aggaatggga
     1681 gtcccattag gtatattatc aaagcttata ggtatatcat cgtcaccctc aagaggcttc
     1741 agtgccagtt tccatatacc tctccatgca acaagaccct gtgatttgga aatgtggaag
     1801 ttgtagcgga tacgatagtc aatagccatg ccaagatgac tatacaccca gcttggaaag
     1861 ccagggttga ttgtacttac atctcccaat gtcttattgg tcactttcgt tatagaggag
     1921 gtctgaggaa atcgctgact gataaactgt ccaattgggc tgtttgcgtc tttaagatga
     1981 gaggtgagtg acattgtttt tgcttccttt cgattctatg gatgtggtac aaattagtac
     2041 cattacccga accatctttt tgatgataca aaatacggaa taaagatctc ccgaaaggtt
     2101 ggtcagaagc tgtaaaatga tgtgcttttt caatcgttat atccctccgt tatagaacta
     2161 tcataaacca aatgttacga gaggatagca cagccgtttt aataagcagg tagaatgaag
     2221 atgggagaga gatgcacatg aaaatgtttc ccgttaggcg ttagagcaga aaggaaacca
     2281 gactatgcat attcatccct atcgtttgca ggtgtggact gttcttgtaa ctggcttagt
     2341 ggcaaatgta ctaaatattc tgatagacat ttcttttaag atgctgcact gatgtatttc
     2401 tgaggtactt ttaggatatg tccgtaaagt gttctactaa tgaccccctg gtgactggaa
     2461 acagaagctg gggggatcat tctattggtt atttggtcga gtattcgcag aagtccatta
     2521 taattatatc tcttcgacaa aagagaagct acaatgacct taagctatag ggggttctga
     2581 ctatcctaga agcgtatcat cgcttctttt ttctttgctg gccgcctgat gacatgcgtt
     2641 cgtagata
//
LOCUS       DAOR01000320           24602 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_25846, whole genome shotgun sequence.
ACCESSION   DAOR01000320 DAOR01000000
VERSION     DAOR01000320.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 24602)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24602)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24602
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_25846"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    11093..11301
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18469..18729
                     /estimated_length=261
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7330 a         4440 c         4404 g         7958 t
ORIGIN      
        1 aaagtggcac ctccaaattc catgtttgtt atcaacaata tctaaccttt ctttcatggc
       61 agtatctctg ctatcttcaa catatctgta tgcttgagct agagcttgag gaccggggaa
      121 ttttagatcc tcaccatttg ttggacatgc agaataacat agtccacact ttatgcaata
      181 ggagaattgt aaatatttct caacgtcttc tggtgtttgt ttgaattctc ttaatttttt
      241 gttatcctgt aattggatat tagcatatgt attatgtata tagggcttca tattcttgtg
      301 atgattaaaa aactgtgaaa agtctgttac aaggtcacgt atatgtggaa aatttgatag
      361 aggttgacag gtaattgtgt tgttaccaat atcttgtaat tcagagatgt tagtatagca
      421 agcaagcctt ggaatcccat taattttcat tccacatgaa ccacacgatg ccattctaca
      481 agagtatcta atgcacaagc ttgaatcaac gtattctttg atataaagta atgcatcaag
      541 cacagtggtg ctacgtccta caggtatttc gaagtcatca tagtagtgag tagcatcacc
      601 tctaatggta ttagccctga atattttaat aaaggacttg tcttgtgtga cttctttctt
      661 ggaagtttca ctaattttaa atcgtttttc acttctctcc tttctaacta gcattccaat
      721 ttttttaatt atctgactcg ttttcttgtc atcaccttac ggtatttact ttggttttaa
      781 agtgcatact taatgccctc attgcttttt taagaagtat tatcaaatac gtattgcacg
      841 ctttccatcc tactttaagc taagctatca accatctata ataaaacgct tcctcttgga
      901 gtataatatt aattgcaaat aattgcatca tagatgttcc aataatatat ttgcatgagc
      961 aaagacatgg taggcaatat atggccacca aatgtgcatg tccggagggt acagttgctg
     1021 gccaaacgag ttagaatccc tagacattag tacaaagaag aaatgaacta aacgacccag
     1081 actttactga aatacgaaga aggttttcct gcatcgagat tacaaaaccg catattacaa
     1141 aaggagctga atttccgtca taccttctta aatgatccta tgtgatattc aaagagaata
     1201 ctcatcgttt gcaagaatga tacgctatct atgaagctta attatgaaat aaattcaaca
     1261 cagaaaaaca ccacaataat atcgatattt gttattatta tatcaaaaca taaatgccct
     1321 tattctgaat tttaacagtg tacgttttta gagggatctc agcaggaggg ttttctggta
     1381 taccgttatc caacttgaaa actgataaat gtagtgggca cgttactgtt ttttgttctt
     1441 catttaagaa cccattagaa agatcagcat attcatgcgt acatattcca tcagttgcat
     1501 agattttatc ttgtgctttg gctatcaaga ttttcttatt attgtagtca aagccaataa
     1561 tgtcgccatt gcctagcgag tctgcttcac atacttttat ccattccatt aatgcatact
     1621 attgaagata ttgataattg tacttttctc tgcaaaacct attgttttgg gatatatttt
     1681 atgtgacaga tttcataatt atggcaggtg ctctattttg gtattataga tttcatattt
     1741 cacccgaacg aagaaaaatg aaaattactg gttaataatg tgactcttaa cttatcctcc
     1801 gaaccaggtc gatattgatg atctgagcag agaaatagga gtaatgctca atttctgccg
     1861 cttatagaac ccagatactt aaaaattagt accaaagtct gatgaaagcc tttcagatat
     1921 tgctgtttta atggatatgc caggctcaca tcttctacct caagcacaat cacgattaat
     1981 agaatacttc aagtctgtag taaggttgtc ggataccaat cagatattct tccccagctt
     2041 tgcacacggt ctgtaatatc tgtaatatct gtcaaatatc ctttttgagc aaattctcga
     2101 aaccacactt gatctagaga gattatatca acaggagttt gattacctaa tgcatttaat
     2161 atctgcgtcc tcgtctgagc atatggagat tgtatatatt tatattaata tccatgtcag
     2221 gatgtattgc tcttaactct tgtaatgcag gttgaaataa gtggactctt tggtctctta
     2281 aaggattacc tcctaaatta tctactacga tagctgtaag tgtgacttga ttttttgttg
     2341 agccaatgct ggtagagtaa tgaagcccgt agaacgagtt actaccatac caagtgctaa
     2401 cacaaatgaa atacaaagaa atgaaaaaat gatactcctg ctgagcatta aagaaccttc
     2461 tgagcaccca tcctaataaa aatttgttca caaaaacttg ttcgaaacca ttctctgtaa
     2521 gttgctccca tatttttagc agtccaacct tttccttaca attcagtata gtttgcagga
     2581 atctatgtga gaggctgtat tcgaaaatca ttgtcggtaa gagccattat aaaggtggta
     2641 agtatagaat caaactactt tacatatgga atgctatgag ttgcagatgt acaccagaag
     2701 aagtcaagag aagaatattt tgcgagatgt gtaaaatctt gctaaagacg gataattatc
     2761 ttaatggatt attttgacaa ttagatagat ctttctgaaa gtatagtgta ccataaataa
     2821 acgagtagaa tggcagctgc ctcgattggt agagcataaa tttgagggat gaaaaagtca
     2881 ctaaaaataa gtggggccag caaaggaaag cttgctcctg catttacaaa aacgtcataa
     2941 gcgatgtgcg agataaatgc tgctaatgtg ataatcagat actgagaaaa tatatccttc
     3001 ttctctggag ctaaaggtga attgtgtacg ctcttgctat acgccattgt cttttttttc
     3061 ttaccggatg atgattcagt tgacatcatc tcattccaaa ctgatacttt tcggaagatt
     3121 tggcttacca tcaaacctat tagaaccgaa gacaggatgg caaaagaaac agaatgaccc
     3181 attctagctt gtatgtcaaa cccagctgca ttgagcaaat gatccgcgtc aatagttaat
     3241 gccattagcc cgctcaataa gcccatcttg agattcctag taggaagagc aactacatag
     3301 ccaaaaagaa aatgtcctcc aatttcttga ggagtaagtg aaccaaatgc atcaaatgtt
     3361 ataatattat ttacctcgtt actaaagaaa ccaataaatg aataaagaaa ggctaatgct
     3421 ccaaatgcta aagctattaa gattatcgtt aagggtttag gagctcgtga agcgatccgt
     3481 tggcaaagta acatattgga atcgatcttc gaagctagca atctgtatac ttctattata
     3541 aggtttttga gcgtttgagg atcaaatccg gatactgaca tgaatcttaa gaccactttg
     3601 ctaagtcgcc ttgcgcaaat tgctgactgt ttttgagatc tggctgaact tgtcacttgt
     3661 ttgtaaacgc atgtggtttt atgttatttt attaattata gatgtgagtt tactattatt
     3721 ctcgagtaac gcggaacagg aacagggaac tacacgatta tcttataaac gatattattt
     3781 ggaagtagaa tagtttttta tacccaaact agacactgct agaaagatat aaaccatggg
     3841 gactgtgaaa aagaaaccaa ttgttttccg agcaggtagt cattcatgtc aaaagcgatg
     3901 gggtaagaaa acgcgttggt aaatatcggc aatatattaa cattgagata tgacccctct
     3961 tctgattcag tgactaaaaa taatgatggt ggtggcatta gaaatataac accaaatgaa
     4021 atatcacaac tgagacaaag tactactaaa gaccaccaca atataccatc cccacaagac
     4081 ctagaaaatg aaattagacg cctaataaag aaaaggattg aaggatccaa agcaaaacga
     4141 ataacaattg ctctgagttc cggagttgat tcaaacgtaa taatttctct gattcgtaaa
     4201 gagtttcccg aggttgatat ggaatgttta aacgtgactt ttgatgagga ttccagcgaa
     4261 gctcttcgcg caggacaaat agcagaaaac aatgaggcgg gatttcacga aattcacgta
     4321 gagaatccat taaaagattt gcctatgctc ttgagcatta tcaaagaacc gagatggaat
     4381 gtatatcaat attatttcat tgagaaggca agatctatct cgaacgtctt atttaccgga
     4441 gatggaggtg atgagctatt tgcagggtac acgttccgat acaaaaaatt tcttgcaact
     4501 ataaagtcat tgaatcgtcc ttcttgcgaa gagagggttc atacgtatct tctatgtcat
     4561 gaacgcgatt gggttcccga ccaggaagac atgtttgccg gaacacaaat taaatttgca
     4621 tggtcttcaa tttatcagct agttaagaaa tattttgata acaatttaga tccactagaa
     4681 caagttcttc ttgcagacta tcatggcaaa ttgatgtatg acttcatacc tactaatgag
     4741 aaatttttta aacattttaa cattgatggt acagcgccgt tattatgcag tcaaatcatt
     4801 gatatatcca tgaaaattcc atcatcttta aaatatgatt atgaaaccaa catagggaaa
     4861 attcagttac gtgaaatcat taaacaaaac atggctggat attcttctga aggaataaaa
     4921 aagatgggtt ttggtatgga tctagctaaa ttttggtcta gatttggaaa ggaaatagtc
     4981 acttcacatt tagacaaagg aagaatattt gaagataaaa taataaacaa agaatggtac
     5041 aacaagtcat tgactagaat aaatgagaat cgagaagagg ctacaagata catgagcaag
     5101 atgttacaac tgctcagttt agaaatatgg tataagttgt ttgttacttc agaaataaac
     5161 gctagctatt caatatgaaa aaatcaagcc ataaataacg attcgtatgt ttttgttcct
     5221 attaaaatag gagtaaacca tcacaggtta ggaaaagcaa cacaacgagt tggtggaatt
     5281 ggcggaactg ttaggacttg tctggctcta aaaccccgga tacatctatg aatgaataat
     5341 tatgtgctta agggtaggct gaaactgaat gtagcacctt taccatccag attatttcga
     5401 gcccaaatct tgcctccgtg agaatctact atacctttag aaataaataa gcccagccca
     5461 gtacctgtgt ttgatcttgt agcaaatttt gtaaacagtc ttgtcgctat ctctggatct
     5521 atgccagatc ctgcgtctgt tacactaatt ataacaagcc cgtctgttgt cttttgtgta
     5581 gtgatagtta tattaccttg ttttgtaaat ttgacagcat tgcttaaaag attcgtaagc
     5641 acctgattaa tcctcgactt atctgcttcc acgttaataa tgtcacgttc ggaggataat
     5701 aattctaatt ttaaactggg attaatattt ttactattat tattattatt atcgtcatta
     5761 tctcttgaaa tttcgttttt aaaatcagca agagtgttaa gtagaatttc agtcagattg
     5821 aatttttctt tattcaattg cagtggtttt tggctttcaa tctttgaaac atctaaaatg
     5881 tcttctgtaa gtcgccttag tctttttgca tttcttatgg tgacatctag cattccgatt
     5941 tgttcgggat cggttatttt agatcgcatt atttctgata aacccagtat gggttgtatc
     6001 ggattgcgca attcgtgcgc agctatatga ataaattctt tttgcatttc atctgccttt
     6061 tgtagttgtt catttatcat atgctcattc caaagtaaat caaacacaga tttaaaagag
     6121 tccacgctac gtttgctgtt agaataaaga gtaaatccga ttgcttctgc aaactcttta
     6181 gcagatgact ctctgagttc tgctcgaatg aatcttgcac tatctactat caaaaaacca
     6241 gaagtagagt tatggccatt tagaattctt atattaggtg cactagtaga tattcttttt
     6301 acaatgtctg agttttcttc actcaaagga caaataatcc ttacagttgc gccattttgc
     6361 gatgccttta taagatgatc gataattcct aatctgtcca cccttttcat tgacctatca
     6421 gctggaagaa ggaatacggc ttccagcttt acagattttg caagctctac aataatctga
     6481 gacgctttta ggggatcggt aataacttca aatatttctg gttctatatt ttcctcgata
     6541 gtttttattc tatcccgagc gtctgtccca tctttccaca attcttcaaa aatggaaaca
     6601 aagtgagtaa tgtatgggta ttcattactt actaagagac tcttaatatt ttcttcgcct
     6661 ccttctgcct tctcaattgt agcaatcatc tctttgtccg aaacagcaaa atcgattggc
     6721 ggcatattct tggcatgtct tacatgaacg cctatctcga gaaactttct aattagattg
     6781 gcactatctt tatctgtaat cgaagtaacc atcctgatgc ccttatgctc cttcttctta
     6841 tacttgtcca atactttcgc atacatatca agatagttat tatacactag tcgtaatcca
     6901 ccaaatcttg aacaaactaa gaactcaaaa gatgactcta aaatttcttt tcccttcttc
     6961 aggatttcgt ctacattttt tagtatcttt atttctttta atactgtacc taaatactgt
     7021 ttcatttcgt ggtctgtaag tgatgatcgg actccggtct cttgtatcag catttcttcc
     7081 tgctcttttg cactcgttaa aacccttctt tctatttctt gctccttccc aggtccttca
     7141 ataacatctg ctagtttaga gacttctaga gcagaattcc taattgattt tcgtaatgaa
     7201 ctatcttggg atatcgaaat tacaagagaa tataatccaa tagtaaacaa ataggacgct
     7261 agtaatacca acgaatgtgc tgccacacca tatgtttgtt gtagggcaga tatctcattt
     7321 gcaacaccaa ttgggacaat acccattgca gaaatggcta aatagtcttt tattcttgct
     7381 gcagttaact ttctggacac tacgtaaaac gcaagaccaa atagaacact actgcctatc
     7441 gtccctgctc tgaatataag tctgtaatag aatttatatg gtatattcgc aggcaacgaa
     7501 aatattaaac cgcttcctac gaggtataat atcaaaggaa tagataggat aagccagaat
     7561 ttcagattta gcttgcctgt tctcttgtag tagtaacgta aaagcatgaa agtaccagcc
     7621 catgttaata tgtacggcgc atccgaagtt atgtatataa tttcgttata gagaggtagg
     7681 ttagcggacg gaacgacata tgatctagta atatggggat ttactacttt atactctact
     7741 tcaccatgat attttgcaaa agtttcgtaa atataagagg cttgcggaat tgctctcggc
     7801 ggtgattttt catctaccac ctgtattagt aatattttat caattgcatc tcctgctatt
     7861 gatatggtta atgctgccgc tgcaagacca tataacaaga ctatgaaatt ttgtttattt
     7921 gatttatacc acgaaaaaaa tttgaatgcg ataaagccca tgataatgct agcaccgata
     7981 gtgctaattg cgtatactga agtaatgggt actcgggacg tattgcatag actaaagtaa
     8041 ctagggcagt ttatgatatt atcaaatata acgtataata aaatcccgaa caacgaaaac
     8101 tgcgttacag ttacacccca atgcgttatg ttaacataag aagatttaga tctgagatct
     8161 cctgttatat gtttagtata gcctaaaagg atccaggatc ctattccgta tgcaattatt
     8221 acagtcagta taaagagtat ggtctgccca gtgttgtcaa gatataaaac ctgacgagtg
     8281 gctagcaaat caatgataac tataatagtt ataaccgctg cagtaacgat ggcagccttc
     8341 ctactacgag ttagaaacgt gaatagattg atgtttctca acatcatgat ttgtcagttc
     8401 ccaatatggg gaaatagtag catccccggt aatatacttt aatagtattt tactggtgct
     8461 gttatcttaa gctcaacaat gaattattgg cttaggttag gtataatcgt tcaggcaaac
     8521 gtgttgcggt agttggcatc tgtgtatatc cgatacagaa ttatgttact cactctgaga
     8581 gattcataaa ggagcagcaa aataaagtcg gtatgacttt acttatttta tctccattga
     8641 gaagtaaatg caactaagaa tctccctcaa agcaaaatga tgctgaggaa actcaaacaa
     8701 agcattcaga gccgcgagct caagggttgt aattaatgac tctatccttc atttggcgga
     8761 tagacgattt aggatctgca gcaattagca cagaccagag tttgctgcgc tgaacgaagt
     8821 aaactaaacg ccaaaatatg ctttgacgtt taggctgtat ggatagtata ctacgctaca
     8881 ttgattgcat accgattata ccacattaca attaaaggaa cataaatttt aatcgagttg
     8941 gctacaatgg aaccatattg tcttcaaata agtcaattat tattagataa cagttaaagg
     9001 aacataaaca cgatagagta cgttggctat gtctcaaacg aaggaaaaat tttctagcgt
     9061 attggtttca attgatggtt cagaagcatc aatgaaagcg gcagattatg ctattatgac
     9121 tgccaaaagg aatgatgctc aattgaccgc cttacatgta ctatattcac aaacaggata
     9181 tgcatattca tcgggaacat ttggtggtct agatactccc agctctataa gagcaattta
     9241 cgaaagtgct aagcaagaag ctcagcagtg gttcgataaa gttaaagaaa agattaatga
     9301 acaaaaaaaa gaagaacaaa aggaaaagaa ccgaattcaa ttaaaaacag agcttgttgt
     9361 aaccgctaca tctgtagccg ctgctatagt aggatattct gagcagcatg atatagattt
     9421 gattgtaatc ggaactagag gaagatcagg ttttaggaaa ctgttacttg gaagtgtcgc
     9481 atccggggtt gcaacttatg cacattgtcc tgtaatggta gtaaaatgaa ttagacaagt
     9541 tgatgttgtc tcatggcgtc atctggacct tttctgctac tgggattgca tgtcaatcta
     9601 ttggaatggc gtttgtatac ttcagattca caactcaatt gataacgtgt atccgatata
     9661 aagtattgag tgtagcttat ttcaaaatag gatcaggcct tctatgaggt tccatttggc
     9721 aattgattta tgaaggatat tacaagctta acagcgaaat gtattattct gtgaactgta
     9781 tagtcgaaaa ctctcatagt gctgcagatc gtcaatttgc actttgggaa atatgttttt
     9841 ggaccgctac catccttaag tcaggagaga caaatataga cattatacct aattgcccta
     9901 tatgttcaaa tgacaatatc tctctaattc ctttatcgag cgatgatgtt cctttatcga
     9961 gcgatgatgt tcctttatcg agcgatgatg tttacgaatt gtcgttgaga tccaaatgtg
    10021 gattgcgatg cgatttacaa aaatacaaaa atgctagata gatcgacatc gtttattagc
    10081 agctgtacca aatgccaaat aaaaaagaat cttgtagagg atgtggtaca ttgctatctc
    10141 ctaaagctac ttgtaatgtt tgtgacgaat ctgtcttgtg gatatgtagt aaatgtggaa
    10201 aaatagacta acgtgcttgc acgttgatat agttggtgga taacagaagt tttttatttt
    10261 gttcctagcg gtgttattaa tccatcattt attaaataag gcatataatg tatttattca
    10321 aatgcttgaa tctgtttatt taattggtct taatcctagt gtattacaat gaacgcaatt
    10381 cgtttgctgc tcgcggagca agtaatttga tcatctggtt aaaagtgatt tcatttgaat
    10441 aatgtatctg ctatatatta tgtctaatga ttgcggaaac ataacaatta aattataaat
    10501 atagaaggat atattagaaa aagtatgcct tgtatttact tttttctagt ctttcccttt
    10561 tgaatgcctt tttggatttg tattatttgt ctttccatct gcttaactga agcattgata
    10621 tcctttacca attttgattg tacatctgag ctttttgcca atttccgtaa cggttgaagg
    10681 ctttgttcca gtcttgctag ataactcgtt tgcttttcaa gttgtttacc tatatcagtt
    10741 atactatatt cttgttcctt tcttctagac tttggtgcct ttttcttctt ctgagtacgc
    10801 tttacctttt gttcctcttc cacttcttcc tgttcaggca ttttctctgc gagcgtttct
    10861 cgttgttctt cctctgctgc cgatccagtc tcatgcattg ctcccatgcc ttcctctgcc
    10921 cttgcttctg ctggctcctg taatcctttt tccatctgct cttctatacc tgtataacgt
    10981 ctttcttcat ggtctgacat attttatata caaaatagag gaaatcaagt tgttaaaatt
    11041 actttctacc acagtttgtt tttttcaccc tactgatata caatattgtg cannnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn natacaatat tgtgcataaa ttagtagaaa taaatgtaaa
    11341 tatactacaa ttattctaga aatatctcat ttcacagttt ttgattgcat tctctagctg
    11401 aagacagtat atgaggccgt atcctatctg attcctccaa tttctcaaat tccgggtccc
    11461 ttccatacca cccttctaca caattttctg gcaggtggat aatatgctaa ggatagatac
    11521 gattcacatg ttatgcggcg atactgaata gtgtagcagt aactcttctg tctactaatc
    11581 tagtatatgc tcaatacttt ggatctaagc atgatctttt ctttatttcg tgtcaaattc
    11641 gctgtaagcc acatcaaaaa cgcagattgt gtccaaataa atacgtctgt gagtattctt
    11701 tggacggctc agacaagcac tcatatcttg tgtaatggat agccacgaac gacttgtgcc
    11761 tgagataatg cattgggagc tgacagagtg cgacattaat aaggatatca aagtcagtca
    11821 ttggtttcat tacatgggat tgaagattca aataaaacac ctagaccatc gcattagaat
    11881 ttacatcaaa tcgatgggag acgatagtgt atgtagaaat ttaaaattga atataggaca
    11941 agatttattc gctcattttt aacaaagtta ctcaactttt tggctgactg gcatgttaag
    12001 tgtaagtgtt gcgcctttac catcagaatt gttctgagcc catatcttcc caccgtgggc
    12061 ttctataatg ctcttagaaa tatacaaacc taatcctgag cctctagaag aattcgtaac
    12121 aaattttgaa aacagcttag tcataatatc tggttgtatt cctggaccag tatctctgat
    12181 acttacaaaa gcctcgctac cttttttttc tgtgttgact atgatgcttc cctctttact
    12241 gaatttagca gcgttactta ataaattgga aagaacttgt gtcaatcgtg ctctgtctgc
    12301 ttctataagg atgcgattag taccattcct tttggttgca ttgtactcta agcctactct
    12361 tccattatca atttcgtatt gagcatcatg aatagaattt gatattaagt catttagatc
    12421 aagtgtctct atgtccagat gtaatgtctt gctctcgatc cttgtcatat ctaagatgtt
    12481 ttgttcgagt cgtagcagcc tcttcgcatt tctaatgatg atgtcaagga cttgctcgtc
    12541 ttctttgctg agcttactgt tgttactctc tcctcttttt ttcataccga agaatttctg
    12601 ataatcctag tattggttgt agaggggaac gcaattcatg tgctgccaca ttaatgaatt
    12661 catcttttaa ttgatctttg tatctcaggt cctcgtttgc tgcttcaagt tggtttgtta
    12721 attcattctg ttttttagta tagtctttta tagaacctac catggaattg aaagattcat
    12781 taagaacaga aagttcgtca ttaccttttt gtttgactga aacatcaagg ttcccctttt
    12841 ttactttaga cgttgcttga gtcagagcaa atattggcct aagcattctt gcaacaagat
    12901 acaagattga cactagaatg ccaataatta gaatcgcaaa caggatttgt aatagtatga
    12961 ggttctgaga attcttatct gtttgctgtc ctagcagtgt aatcagaaga ttcaatcaag
    13021 ttggatgcca tcgattcgaa tcctttcctt gttagtgact ggtcagtagt ggttctcgct
    13081 tgaggtgatg taagcagttt attagtaaca taagttttga aaccattcca tcttccatca
    13141 acaatattcc agagatccaa aaagtttgaa ggaagaggtt ttagatctat acctgaaatc
    13201 attcctcctt gtttcagggc tatgatattt gattccaagt tattcatagc gacttttagt
    13261 tgtgaggtat cggatgataa tccatacaga tattgattga ttggaagtaa gatatcgatt
    13321 cttgccagca atattaattg aatttcccaa tgatgattgc tgagactgaa aatatgctag
    13381 cacaccgaaa ctagcaacaa ttaaaataat ttctattata accaagagtg tgatttttaa
    13441 gagaatttta gcatttatcc ttcgtagcca ggtcgacaac taactttaca cgcctctcgt
    13501 cctgctattc atgatcacta gggttctcca tccaatccat tcaaaattgg catgtcttct
    13561 tcattcctga tctatttaca ttctggtagt tgcagatata gattactgaa tctgggaaca
    13621 atctcagata attgtctgtt tcttgtaacg aaattgaaag acaacaacag aacttattag
    13681 cattcgagca tgtcatcatc aacacgattt agtcttttat catgtgttat atcagagaaa
    13741 gtctagctat gaatctgaac tcaatccaat tctgccttaa ttcgtttaac caaataatct
    13801 attgtcactg gctttttgag aaaacatccc aagcttaatg ttggataaat ttctcttaag
    13861 gcttcccgat ttatttctcc tgaagtcatg aagcaaaccc tgacattgat atctatttct
    13921 aaaatctttt cacataattc aaatccgttc agatgtggca gatcaatgtc tactaatagc
    13981 aggtcataaa agtctcgttt gaattcggat agtgccacta acggatcatt atagggataa
    14041 acctcgattt tcttattgtc gttgctagca tcattattgc tactttctat gcctaacttg
    14101 aatgtgacag taatatctgg ttcatcgtct ataattaaga tttttttcca aaatcgttgt
    14161 ttctctctta cagtaatggc cccataagat agatttcttg tatattcaaa aaatttcatt
    14221 ggcatgtaat cagtcctttc gcaaattcta ggttatttgc ttctaatttg tatagtcgtg
    14281 gtaccattga taatttcatc tatccttctt ctcagctctt cattaaagat gagactcaaa
    14341 attctccaaa gtgatgggag aatattcatt agagtcagct ttgaatccac ttggttccag
    14401 cgctctagta atgcagcatt agcgtcgggt tcatcgtcaa caatgagaat cctcgttgac
    14461 ataattattg gtcgatagcc tcctttccca tcattctaca ccattttttt cctggttctc
    14521 ccgctgtccg gattacctat ttgctataca ctcactaaag ctaactttac ctgcttaaac
    14581 atacagggct ttattgattt gtcatgaatt ttgcaggccg taaagtgcca tgctgcttca
    14641 agtgcaagga agacagttgc aaagccgagc cctaaatatg ctatattttc taacacctat
    14701 ggtctaagat gattgaaaga tataaggctg ttggatataa tatttaacaa ttgaaaagta
    14761 ctgtatctgt aataatcggt atccctaatg tgtaatatcc aaaatgttgg ctgtatcatc
    14821 catttgtaag cactccccgt gcgatgtcaa catcgaaaac ctgtagaaac gtcctattct
    14881 cgtttgagga cgcatggcaa caatacgaga gcacgtcgac taaattggtc ggtaaagaat
    14941 tcgtgtggta tcattgcaag tatagcaaga tcccaatgtg accattaaaa tatattcgat
    15001 gtgattcaat aattttgtaa atcagttttg aaatttgaag agtatagtca gacattcaaa
    15061 tcacacattg ttatcgacta agatgaattt gaaaaatatg tatacattta caaagacaag
    15121 gaatattttg catgaaaaac ctgaattggc cgccgacgaa gggattaaag ctacgctgcc
    15181 tgccaggccg agcagtaagt agcatgtgtg atttatcaaa aaccttacct atgaaggtaa
    15241 attagtatcc ctcggtacag tcttgtatgt ttgaatatct gatctactca gcgattggat
    15301 ttgtcggcac actcttagca ctcgagatag gtgtgttaga ttatacagtg ttactacaaa
    15361 aattatagaa aaaattgaga taatttttca tcaattaaaa atgtgccatg gcaaatttaa
    15421 ccaaaaaaag agaaacacct tcacaaacag agtattattt acaaacatga cctggcaggt
    15481 agatcgtgtt tctggtgtgc atcattgcta ttctatgaca cagttattaa atgtcctagc
    15541 tgcaacagcg acacaataga attaactcca atttcccatg atcaagttca cagacttcaa
    15601 cgtactccag ggtatgggat aagttaacaa gagtctggag gttgacaaga gtccaaatag
    15661 cctgtaatac tcagtcttgt gttcttattt ctttttgaaa aatgctgtcg ttactgtccc
    15721 cttggactat acaataatca aaaaaactga ctaaaaagga ccattacaac aaaatgcgta
    15781 tagaattcaa aaaaattagc agccggttaa atctagacga gttgctagta ccaccgttca
    15841 tgtagcggca acacaaagag gataccgatt aagactaatc cgactaccat tcctatgaca
    15901 tagatgttag acttgatttt tgatttccac atgtgttata gtctgtttct accagtatta
    15961 ctattgtact cagctgcgtt gatgagaaat cagcactttt gctaataaag agtgagaaca
    16021 taaggctgct ttactttgta tttaaaagca catacgaata aatttgattg gcctcgggca
    16081 tggaattaac aagaatgctg ctcgcatcga ttagtaatcg caaatgcatt aatacagcct
    16141 aagcagtatg tttttacttg ctaccaaaaa cattcttgat ttaatcaatt ggagggaagt
    16201 aatcaaataa aaggactgta gtgggtttta tacggattat taattattta ttactaatgt
    16261 ttggacaaaa tgaaacactc cttagggaat attattggac agatacagca acaatgatat
    16321 cagctctccg tatagaattg atcgatcaaa aaagaactgc cctgagtgta ggggaaaagt
    16381 cggacaataa aaaattgccg tatcctgaag catccagagt tattattaca aggaacgcgc
    16441 agactgggga tcttctatta gattatttac aagatgataa gcgagtggcg caggaaataa
    16501 tatcaggaca cacaatttca agagtatcag tcgaattccc ctagattcgt ctagggtcag
    16561 aacttaaacg ttgtggtggt ttcttgggaa ctctggatct atagagcgac gtcgccagct
    16621 tcttttgagc ctatatcata aggttctgca atatcagtga caaatacctt gccatcccaa
    16681 ccaggaccac gatattacta ctatggcacg cgatacaccc ataaccaata tggtcaatac
    16741 aacaggactt aggtatctag gtccttgaga tcgctgtgcc tcgtgatcgc tttccatcac
    16801 atctcccatc gagttttatt tgtcattatg ttcaatatta ctctgcagtt tagattttag
    16861 cagtgtgaga tctatcgttt atttcaaatc gcttcttcat ttgcttaaga aaatctcacg
    16921 aagcgttttt ctcaatcggt ctcttgcgct ttccttcagt ctgcagttta gagctttttc
    16981 tagtcccctt tagacaagac agaaatccca aaactgctaa cggcacgcct atgccgaatg
    17041 caggatatat gccagtttct attgacaatt ttagaggtca cccatgacaa gtatgtggta
    17101 tttataatat ataaatttga cgaaataaat tgttatttga aatttcactt tgatattacg
    17161 cctgacaaga atcgatttat aatatttgag taataaagct caaggaatta gaatatccaa
    17221 tcttcagatg cctcgtaatc ataatagtca taataatatt caaggcgtaa gtgtgactgg
    17281 agccgcaaag tcagcattcc aaccttggat tttttaaatt aatgcctcga gcaagtgtca
    17341 ttcggacttt cgaattcacg aaccaagtcg atcaaattag tcatattcgg atatgctttc
    17401 tctatcctgt actccatggt gcatgaactc ttcccatttc ctctcccagt tattacatat
    17461 tactgatatt gctagtatct cctggcccat ttccaataga tcaggtactg tgtactgatt
    17521 gttgggagac taattgtggt gctgattgag gtcgttgaac atattgttct tgtcgttctt
    17581 tattctttag ccgttgaaca cggcttgtta atcgctgttc ttcctgcttt agcttgtcta
    17641 cttctttttg gatgaatctt aactgattat ctgcgtctct tagtgcgagt aaacattctt
    17701 tggaattatt aagctcattt attgcgtgtt cctgtgcagt tgctacctca ttggctaaga
    17761 attcttgatt tatctacctc actcgctaaa gatgcctttt gccttttccc ttcaggctta
    17821 ttactgttat ttgctgctat cgccacgatt cccttattac tttgctgttt ctgctgctac
    17881 ttactgctac ttttcgtatt ttcacacgag ggatctagca ctacatccgg tttaagaaaa
    17941 ttatttagac atcaccgatg ataaaccgga gatgataatt aatgaacaca gcaaagagtt
    18001 tatcaatagt atcgatatag aattggagga atgaaaacat gtgaaatttg tggtggccta
    18061 ggatcatctg gaacccctaa cactatatgt ccattttgta gcggtactaa agaatctaca
    18121 tcggcttcaa aaggctttat cgaacatcat atttgtggtt gtaataacac agatagaagg
    18181 aattgcccct ggtgcaagaa accgtgtcat catgatgcgt ccttgaaccc taggatatta
    18241 atttcatcaa tataatattt agtccacaac tgcttcagtc ctaataagaa atggcggcgg
    18301 acttcatgta aaaactagat tggctatata taagatattt atgaagatat ggtcaaattt
    18361 cgcacagcca tggaaactca gatagaggct agaaatatga tggccattct aggaaatctt
    18421 taaaaatatg atagcttgaa gatgtactaa tgtctaatga tgagatgcnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnna gcaaatttca aaaaacaatg gacgatatat ttaagcaaaa acaaataagt
    18781 caacgcgaat ttttcattca gaaaatcaaa gaagaacagg aacatgcaga ctcagatatc
    18841 aaatcaggtg acctaatgag tgcgatggct cataaattaa cagctgattg gtattcatct
    18901 atgctaacta ttacgattca ttaggttact ttattctaga tttgtttttg aaacttagag
    18961 gatttagtaa tccttttagt ttgttatttt tggctccaac tgagaactgg aaaatcaggg
    19021 aaggtaattt caaagtcttt atgcctgtgt cgtttcctct gaaaatagaa caagatttct
    19081 ccggtgctgg tgccggaatt aggcaataat gtccatgatt tctcggctgg agatcaattc
    19141 tttggttttg ctcctagtgg agcatatgct gaatatgcgg acgtgtatgt caaagaatta
    19201 gcacacaagt ctgaatcaaa cgattttgta acagccgtct cagttccgac agcaggcgtg
    19261 gctgtattgc tcgttcactc cgctaactta ttgtaaatct aacacatata gtttgacttc
    19321 atgcataaac cctttaacat agcatttcta tcgaataata aatgctgaaa cgagaaaaat
    19381 ctagaaaaaa tagtagagtt tacgaagcta ttctggcatt tcttggaatt ttcatattga
    19441 tattggcgtc agcgttaggt gttaataccg gtattttatc tcatcatcct cgcctctttc
    19501 ctctaacgta tattggtacg gccgacgcac agacaagaag taataatggt tcgttgacat
    19561 tattacttcc ttatcaaaac ccggcctacg ggataagtat acagtatccc tttgattggc
    19621 ataaagaaga aggcggtcaa accaaagcag gtaacttgtc tgctttgtct gttgcattct
    19681 actctcctcc tcctttaaaa agtgcatttc ttaaagtcat agaattaaat tctacacaac
    19741 ctctgaatga cacgatacct cagcttttaa ctgcggctat agctagagac atgcatgtta
    19801 ctagaggttt ccagatcatt caggcaagca cgaatggcac acttgcaagt aagcctgcat
    19861 atactttggt atcgacagga gaaattttca agaatattcg atatcaaaca atggaagtcg
    19921 ggacattatc gggcaatagt aaatacacca ttatatttga agctctggca tctaattact
    19981 ttacatacct tccaactgca cagaaaatga ttgattcctt taagctacta aaataaaatc
    20041 ctgtctacat ccggaggcta attgctctca cctccacgtc caaagacagc aattttaaaa
    20101 attgctattt gtaataaata gaaattatta ataccctatt atctgagcgc tgggtagtaa
    20161 ttaattactg ctaagaatca gcagtatgtg ctatgagata cttgaaaatt ataatacatc
    20221 tatatggcct cgcttcatac tccaagcgac tctggtacaa caggtggcct agaatgcgaa
    20281 aacaatgaat gcagactacc gacaccaaca tgtgtcgaaa attctcaatc tgcctctttg
    20341 gggactgagg aatggtcagg ttatgacgtg tgccttcgat tccggtggta aatgctccga
    20401 cgttgtctga tgggtgcgta tggccatgat ccaccataat gtaacacaaa ctcttattgt
    20461 agttcgggaa ttacctgcta ggacgtcctc ggatggccgt cgaatagccg ccagatgagg
    20521 tcaaatgatg gtcatatggc agtcaaatcg tcttcagatc ggtacctgtg ccttcttcca
    20581 atttgatgcc ggtaacttgt agaagcctaa tggttattgt taacttttgc tgatgccgat
    20641 cctgatgttg atgttgaagc tgatgccgat cctgatgttg atgttgaagc tgatgaagat
    20701 gctgatgctg cagccttgaa tgcagaatta tacccaggtg cagatgcttg aactgtagct
    20761 ttaaaatcgc caatattatt ttttgagttt ggttttattt cgctagaata tgtaatctct
    20821 ccgttatgac ttgagatttc tttagttgta ccattagagt taaaggtaat cttcattttt
    20881 aagattgcat tatcaatttt cttgcctgaa atagcatcat aagctatggc tgacatgatt
    20941 gattcaccct taccgttaac aatattctgt gatgattgaa ttgagataga caacggtcta
    21001 gcattactac ttttagattt tgatgttgat gctggtaatg gtgatggctg ttgttggtgt
    21061 tgtattaatg gctgttgttg gtgttgtatt aatggctgtt gttggtgttg tatagttgca
    21121 tttgttattg tttgattatt cttattaact tgtgatgatg gtgatgtcaa tactcctgtt
    21181 acattcacac tgtaccattt taccaagtta ttagtgatag tactattagt tgaattaaca
    21241 ctactacctg ctggcagaca agataattta cttgttatct tattttctcc ttcttttatg
    21301 gcactataat taggtgaaac tgtcaaatgc catctggaat agtcatttgc tccatttggt
    21361 ccatctgctg tagcatcttg atacggtctt atattattta caataattga aatgtggcag
    21421 cgattattac tgttattatt attattgcca atagcagaag tattggtgcc ggtagatatc
    21481 cctgaaattg taaggttact accaattgaa acgttttgac cgttggtagg tgatattatc
    21541 ttcacgctag gcaattcttt ttgaccttca gaaaaggcag ctatactagc gcatataatc
    21601 aaaggaataa ctatgactat tgccaaaacg aacagaacag acgaagagaa tgctgatgat
    21661 aacaatggct ttgatttcat caacatgttt cgctcttgaa atatcaaatt ataaaagata
    21721 ttaatatgtg ttctcacgcc cgggtgttct gctactgctg gctgtccgtc aatgaatcca
    21781 ttacgataaa cgtcttgttt cacatgatcg acaatgcgcg cgtgattaat cacaacagga
    21841 agtgacttaa gaacttatta atcaaacttt ggaaatacta tctgttttga tggtgcataa
    21901 tattaataag agattaataa ttctcgtttt tagctttttt ctcatattta acattgcctc
    21961 tagtggaggt cattttgata tctgggatgg ggtagaagca tttcttgtaa ctgaaagcat
    22021 ggtcttgaaa cattcagcaa aactgtatcc tgacctaccg agcataaaga cactatcttt
    22081 taatatacgt tatgtcgtct ggttcttgaa atcatctgag gacaaaaaac tttacaatta
    22141 cgaaacaatt ccacttgagc ctgtatattc agttagatca ttatttctgt cggcaatagc
    22201 tgttcctttt tattatctgg ccaccatttc gtcaatctct ctggtggccg tcgttggact
    22261 ttttgtgaat tccttgctaa tttcattaac ttctttagtc atattctgtt tttcattcga
    22321 ggtttacagg tctaacaaaa tagcagtcgt cttaagtctg atctttgggc tttgctcctt
    22381 tgtttgggcg tacaacacta ctttatggcc gcaacccctg cagacacttt gccttattgc
    22441 atcaatttat tttatttacc tgtcattaca tcgtcactct tcatttatct gtcattatac
    22501 cagagttaat cgcaaagata acaatcatga tacttacaat aatgctaaga gagacagtat
    22561 caaaggaatt tattttgccg gacttggagg attatttctt ggcttatcag tatttgctca
    22621 tcctagtagc cttattctga tcccaggatt tgtagcatat tctattttct ctatgagacg
    22681 caatagcaga gaacttattt cttttgtgac tgtattagcc attatcctat ttttaatagg
    22741 actcgttaac tattcgagat tcggttcctt tgctgagttt ggttatttct actacggaac
    22801 attatcagca catggtggat ggattggctt gtgggggctg ctagcaagcc cgggagcagg
    22861 attgattttc tatttcccaa tagtaatttt gcttccaata gcattcagat acttctacag
    22921 agagaacaaa ggactattct tattattctc ttactttctg atcgtcacat ggttgtttgc
    22981 aggtacatta tatttcgggg gaaggcaggg agaccgggca tggggtgggg gggctgaagc
    23041 gtggggacca aggtatttgg taccagtact accattcatt acagttgtac taggaacatt
    23101 attcctacac atgaggaaag gattaatttt aaaacttgca ataataatat cactatgcgg
    23161 tgcaggtttc tatgttaatt tgcttgggaa acttatttgg tacggtttgg actatagtct
    23221 ttacttgcag acgcatgcac ctacgattac gactacgact agtccctacg atcctatgac
    23281 gtggaatccg tcctactctc caattgtact ccatgcgaaa gccttgatgg ataattacgc
    23341 ctcttatcta cagccaacaa tctatcattc attcttatgg aatacctatg gacttgctcc
    23401 ctgctcatat gatatttatc ttttttgcaa atttggaatt ataccggtct tgttcttatc
    23461 cgcagctatt gctgttctag caatattcat aataatggaa ttacgaacac gtagccccca
    23521 gaaaaaggaa caagtttaat ttgcagacct tatttctcaa ttaagaaacc ctattgagga
    23581 aataatagag gaaataataa aatcggatgt tattaagctg ccaaatccac ctaatataac
    23641 tgaggtcttt ttggaaaatg ccattcaggc gttgaaaggt aacgatatta ataaagcagc
    23701 taggcttcta tttctgtagg cttctgcttc tgtaaataag ccaaaacagt catagcccgt
    23761 acttgcgaac gaatgtagta gtagctcgaa ttgccaactg cccaaaagag aggcgcaaaa
    23821 gcttatgacg caaaactcat gagcatgggt atcagacgac ttagactgag atatgctcat
    23881 caacctatta ggtacctttt gaaaccttag gtcattcagt gaattcagat tcgtcatatt
    23941 atgaaatttt gtgtatgata gaagcgaagc tttggttcat gatgattcca atagaaaaat
    24001 cagttgtaac aacacattcg cgatttatta gaatgcttca ttcctttcgc tttcacggct
    24061 cgatattttt aaaatctgag cgccgaaacg aaatatctag agggaatgac aaaggaacgt
    24121 acatctccct ttggttttac acctttatga aaacagctcc tgatttgata ggtataacac
    24181 cacaagttga cacatagatt ctatttgggg aataatttct ttagtggtct tccaataaga
    24241 ttcaaataac aaagtatgat ataagggagg tatcattatt agcatactct tactgtccag
    24301 ggcgtcaaaa aaagacagac ttgttcataa ttgttcttga cttttcaaca caactttaca
    24361 ctaattccaa aaattacgtg ccgaaatgta ttttaaccaa gatagtaatt atatggagat
    24421 gagcattttt gtcaagcgta gaccgtatct cggcttaatt gttaccattc ttttaattac
    24481 tctgatttat gttccatatc agaatctgaa ttcccgggcg caagcagcgg tagactgcaa
    24541 cgctaaccca agcgacccat catgttcgcc agctgcagct gttccaactc cacctcctac
    24601 aa
//
LOCUS       DAOR01000321            3006 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_99873, whole genome shotgun sequence.
ACCESSION   DAOR01000321 DAOR01000000
VERSION     DAOR01000321.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_99873"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          928 a          600 c          556 g          922 t
ORIGIN      
        1 agagttcgac cataaaatgc gactctgtct aattgaaatc gatttccgtt attgtcaggt
       61 tttttgtcca ttattctact cttcgggcag tctttatgtt ctgataaatt gtatgtttat
      121 agtagtttct aagtttatcc ttcctgacaa atttgaccca atttcgcaac ccaatattgc
      181 aactatccga tgatcatgtg ggccaggaat ccatttccat gatgtgacac tagcaatcac
      241 ggtaccagac gcatgcaaaa tggtagttta tttccacaat gtttgtacca tgaataaatg
      301 gaaggacggt cccttgacac gagtttttcc cggtacaatt ggaattacct tgtgggaaaa
      361 tatttgcttc ctcaaaagac catattggtg gtcctattgg atttattttc aagggatcta
      421 ctcctgcttg tattttgtac ggtgttccat ccggccagta tcccggcgct tctatatcgt
      481 gtctttcacc acaaccagga gtgaaagcac atgttggtcc atgcagctgt gctacaagtt
      541 tgtcgccctt aaagacattg atgtacattg agtcgctcgg ggggcactga atcactttgc
      601 aatttgcctt atcataaatg gctacctgaa gatcacccag tgctaactta gttgtagtag
      661 acgtactgtt gctgcctcct atcaaagttg atgtcgaaaa tgctagcgaa ataatacaat
      721 taattggcac tcctccacta gcagtaccgg aacatcctga agaataggca atattatagc
      781 cagaagggcc tgctccagta actttgtatt ttcctgcttt taatgtaaca gaagtaccac
      841 ttgtggaccc agaaaacgat tttggtgaag gacttttgcc agataccgtt atggtaaagt
      901 ctgaaggttt tttcctacct cctttagtat tgtcaacttt ggtaacaact ttaagaaatg
      961 acgatgatcc tattggagat gttgaattgt tcacggaact accacctttt gaagacgatg
     1021 agggtgaggt tgtctttgtt gcattctttg ccgcaaaaac cgcatggtaa ttactcgaaa
     1081 caaaactcgc tggacctgcc attatcatca ataatatagt tagtgaaact gtaatcgaga
     1141 tagccaagta tttgcaattg ggatttttat cgatattcac aaatgttata gcagcatgtc
     1201 aagtataaaa aaacaatgta agattatgct gaattttcta cactctataa gggaggtttt
     1261 tgcttacact ctttagattt agagtgtgat tcaacacaaa tttggcatga ttttttggcc
     1321 cccacatacg tttcgcttct cctctgagta ctcatttaac attcattaca gtggttaaaa
     1381 atcgctatgt aatctctaac ctaatcagcc cttcttctga agtcatttct ctgcgtgtcg
     1441 gtctttcttg acttctctta ttacttgttc taaatattct ttcatatctt cttcagtaag
     1501 agaagactga atccctgttt cttctgccat tctgtcttga tttcgttttg tgaatgtaat
     1561 caccttcttc tgtatctcgt cctccatctg agccatacca atactatcaa gtaaccttga
     1621 ttccttcatg gcaaatcttc gaattgaacg acgtaaattg gagtcttctg ccacagaaat
     1681 ggctgaagaa taaataccaa tcagtaaaag ataaggtgct aatccaaaaa acgatactgt
     1741 aactaatccg aatggtggat aagggataag aaccaagcca gcagcctggt tagaagaaaa
     1801 cagtagcatc ataccgtatg ctgaaatcat catatactgt ttaactatat tacgactcat
     1861 gttacgtgct acactccaaa atgcaattcc aaataagatt gcaccagccg gtaccgtcgc
     1921 gctaaaaaac agagtataaa cgatgccaaa aagaattggc tcagaaagac gaaggggttc
     1981 gaatacgttc agaaacagag gttgaaattg gctcaaaaaa taaaccaagg ggatactaac
     2041 aagaatccaa tatttggctc taccaagttt tctagagtaa ttatgtaaca ggagcacagt
     2101 tgcaacccat gtcaaaataa atgacatgac agaggttacg acgtagcccg aattgaatat
     2161 gtcatagaca ctgctataag gggctacagg cgttatttct ggttgtatat tcgcgggttt
     2221 actcgtcagt tcattagtaa cataaagaac tgtgaatatt gcatttattg aaatcataat
     2281 aatggctaga gagtaggcta acactacagg attatggtta gacctaaacc aggaaagaaa
     2341 ccgttgcgct aggaatccca acaaagcaat tgctaagatg taattgatcc aaattacgat
     2401 ttcaaggaat agaacattgt aactcgatgt gaacaacatt tccaagataa tggaggcaaa
     2461 aattgctatc aacgcgtatt gtattattga tactgcccta cctatcgtac ttagccgtag
     2521 ccttctattc gcaattcttt tctcattcac tcttctaaca aaccccaaaa taagatactg
     2581 acctaccgca aagattacac acataacagc aaagatcaca atacttgaac ctgtagcgct
     2641 tagaccacct ataatagcag aaattttcac gatagaagta tcaactatta aaataattat
     2701 tatgaaacaa attagtacga aaatgccctt ggagccgatt ttcatctgga atttagtcaa
     2761 acccggcctc ggtattatga ttgctggtca agactgctta ttaactaatt gattgaatca
     2821 acgaagtcta ttgaccttac acatgagcct gtccctgaaa aatcagattg gagattatgc
     2881 atactatcaa actttttata gttacggatc cgcctatatt tgagttagat gagaggtaaa
     2941 ggagaatttg ttcaactgca acgatataga tgtgtgttgc tattatcagg aataatattc
     3001 actctc
//
LOCUS       DAOR01000322           15985 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_44437, whole genome shotgun sequence.
ACCESSION   DAOR01000322 DAOR01000000
VERSION     DAOR01000322.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15985)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_44437"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    923..1137
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9288..9368
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14568..14760
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4924 a         2870 c         2993 g         4709 t
ORIGIN      
        1 gacataacag attacaacaa aaaatactcc tagtttctct agctatactt gccggtatag
       61 ctgcaacgag tgcgataagt tcgcttttcc tagtttttcc tggtttagtg ttagataact
      121 atcccttaca tagaccttat gaaattcctc cactcatatt atttttagtt gctttatttt
      181 atttttataa aaatcgactt gataagaaaa atgatgtttt ctataaagga atagtagctt
      241 ctatactaat tgatgcattt gcagagctta taatgtctta ctcaagtgct tcatttgata
      301 cagcacataa tgtagctcat attctcaaag atgccgctta ttttgttaac attattggac
      361 ttgcgttatc gagtatcaat tataatctaa gattaaaaga aagtaatgat agcttagggg
      421 aaattaatgc tagattgaga gaaagagagg aactgatacg tagtcaatat gagagattaa
      481 aggagtcgga taagatgaaa aatgaattta tcaatatagc ctctcacgaa ttgcgaacac
      541 ctattcaacc aatacttagt ctgtcagcac tccttcgacc aaaaatacga gacgtacacc
      601 agcaggaatt attagacgtc atcataagaa acgcaaaaag gcttctacgt cttacagaag
      661 ctatactgga tgtaacaaag atcgaaagcc agtcattaac gctaaaaaaa gaacgattcg
      721 atttaaatga cgtaataata aatgtaatgg atgatatatc attaagtacg ggattcctta
      781 acaatgaaaa tataaagcta tcatatgagc ctcagcatat catattagag gcagacaaag
      841 gaagaataat acaagtcatt tctaatctac taggtaacgg tattaaattt accaaagaag
      901 gtacaataac cgttacgtca gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc
     1141 cacaattatt cacaaagttc agtacaaaat ctcctttaca cggcgtccaa atgggaactg
     1201 gattgggtct ttttatctct aaaagcataa ttgaagctca tgggggcagg atttgggctg
     1261 agaacaacaa tggttctgac ggaacagaag gggctacatt ttgctttaca ttgccgatag
     1321 ttaacaagga acaacaaaaa aaacgacaac aactaccatc aagatagtag agcttaatac
     1381 ttctatccac gtatcttttc cctagacttc ttctttacaa ccagaagata tgaccatagg
     1441 ttaaatccta gatatttcag attataaatt ttttaatcca agttgggcat aagagactct
     1501 agaaccctag agattttcgc tttattcttg gaagtccttt caaagacgat gtatctatag
     1561 ggttcaaatt atataaaaat agcggttata atctaactag attgattaga ttgacttacc
     1621 cgcgactccg atgttggata cccacgttct gtggttaacc tattaactgg attccctgct
     1681 tcctgttgct gctgtcgcat ctttctttcg tactctttat tcatgagttg aatgcacatg
     1741 cgattccaca cattaccggc ggtaatcaaa caggtcttgg caagtaaacc aatcgacggt
     1801 gattctattt tttccctttg atagtaatcc tgtgccatcg cctgaaaaaa tggtatgtac
     1861 gcacttggca gtctaaggaa aattctgttg ctattattga ctttttgtct tatttctggt
     1921 agcccttgcc cttcacgtgt tttagttact atcattgccc caaacttgaa acccttttcg
     1981 acacgccatc cttatataat tatacacgat gttacgagaa ggtgtcaacg cattgacact
     2041 gtcaccgggg gctgtcagcg tcatcctgac aatgtcctgg atcttgactc tatttgtctg
     2101 taattagccc tccactgatc tataattgca tcatctttct aatcatctgt actacaatga
     2161 atacatctga ctgaggattg tccgtcatga atcgctgaag ggaataatca ttatttttct
     2221 agtatacagc acatatgaag ttgacgaccc atggttatgt agattagtga tgtaaatttg
     2281 agatgaaaac actttggtgg tattgatacc ggggcaagtg cgatcatcgt tatgggttag
     2341 gctgcctttg cccgcattat tttcgattca aacctattgc cagtatgggt ttgctgcttt
     2401 agcccgaaat tcttcttgaa tattccactc tccatcatat gcattcttat agatactaca
     2461 aagaaacgga ccctctgtca actgtgatcc gttatcagaa ataattaccg gacacgtatc
     2521 gttgaaacct gtgtgatata ttcctgtgtc attgactgct acgtccagtc ccaaaaagtc
     2581 ttgctccgcc tgctgaatgc cacctgactg aggatcggcg tcccactctt gtttgagtgc
     2641 atggcttgcc gtcgcagtct ccagtacaga tatcagcagg gttacactac caacaacaac
     2701 aataagaaga aataatcttg gatggaaccg gtggacatgt tgttgggttc ttatcgaata
     2761 tgacgaattc agcgctctgt taagtgacat gaatgagatt tcttagaata gttgctgcct
     2821 tattaaaaat atgttaaatc accaattatt aggaaaccga cagaacccct atatctataa
     2881 tatatgtgga atatgaaaac aaggctcaag cgtagtaaaa atagccctag cagcagtagc
     2941 cgtcgctgaa atagccgtag caacattcct agcaacaata tatcttccag ggacagcaaa
     3001 ggacaacgcg actgcagacg catttctttt acattgtatg ggcatagcgt atccgttaac
     3061 accagactat cgtcctcctc gcggaaataa ataaaataaa gagacaggga agagcaagtg
     3121 taaatgaaca aagaatgccg tttgaaaaaa aagagaccac agataatctc ttcttttcgt
     3181 ggttgctgtc cgtgtcccat cttagggtat caatttcgtt tgtaatcatg tcttatggtc
     3241 ctgtcggttg gggattatca ttgttgttgt cttgtggagg gttgctattg tctggtggcg
     3301 gtgttggatt attgatgtta tctggtggta tctgattgtc agaaagtggt tgttggtctt
     3361 gtggttgctg ttattgctgt aatggacagg tatcagtaat tggaattact gatccatctg
     3421 aacatgtctg agtttgagac tgttgttgtt gcatcgtttc ctcggactca aaaacgtaag
     3481 tcatgtgagc ctgcaagaca taaacgaaga gaggtttgca atcgcaggac tatatggaaa
     3541 acttgccaat accttcgcag atttgaaatc agacagatta actacgactg gtaatttcaa
     3601 gatgctggta tccggagatt tcattagagc gcaaaagaag tatggtcagc cgttcgagtt
     3661 ttcgaattac gccaaattaa tttttagcgc aaacgaaatt ccgcaatcag atgataaaac
     3721 gtttgcctat taccggagat ggataatatt cttctttgag aaggtttttg atggtgagga
     3781 taaggacacc aatctaattg ataaacttac gactgaggct gaattctcag gattgttgaa
     3841 tctggcatta gtcgcactta gacaactaat caaggataac gggtttatcc acacggacga
     3901 cattgaaaca atcgagaagg agtataatct aaatgctagc acagcggagc ggtttgtgag
     3961 ggaaagatgt caagtcgatg tctctgacag agaacgctat gaaatctgca gagaccttta
     4021 ccatgattac gttgtatact gtaaagagaa cggtataact gtcaaggctg ttaacgtatt
     4081 cggaatggaa ctggctcagc agcatataaa gaaggaacgc aaactggtaa agggacaaag
     4141 ggaatattgt tatgtcggaa tcaaattcat aaactcaacc tgactataga ttttcgtcta
     4201 ctaccaaatt gatatggcgt ggttatatcc gcctcgttcg atgaggtgtt ccttaagaag
     4261 tgaatccgcg ggaaccgatg aagcggtaag tttcgacccg aactgacgat gtaccttcga
     4321 gtgtaatgtc acatgtatat ttcccataag atacatttct gaccttatta cggtattttg
     4381 gcaaattctc aatccaaacc tgcatgtagg tgaaatactg ctactcaggt aaattaatgg
     4441 tagaaggatc aggagccaca agtgatcata tcctcactat tgataagtaa aagaagaacg
     4501 gcagcatata tgaggaaagg aagaaatgac ttcgagaatt atagtcagcg atacagctac
     4561 aacagaagga caacgggtgt acaatcagat aactgaccct tcccgagtct gtacgaaatg
     4621 tgtacatttt tcaagtatgc acaatacata gacaaattca cagagcaata agtatggatt
     4681 tcaataatgt aaagccgcca tttaagataa cagacgtagc aaagatatgc gaacaccact
     4741 ccagagagat tggcatataa ttagagtata aatagggtta gactttggac ttttacaata
     4801 aggagagcaa gaaatgacta gtcattcgat ggaaaacaac ttagcagttg tgacgcttca
     4861 tgatgccgcg cctgcgttct caaaaaggat attcgaatat acagatgcat ttgagaattt
     4921 ggaaataaat ttcaatgtcg gacttattcc attttatcat catacggagg accttggaag
     4981 gtttccagaa ttcgtggata aactcaagtc atacaaacga tgtgaaatag tattacatgg
     5041 tctttatcat gaagacagaa acggtcagat ggacgatttt catgccaagt caaaagcaac
     5101 tacagaagaa gaaataagag caggcttaga gatatttgaa caggtaggaa taaagactaa
     5161 tgtatttgtg ccaccgcaat ggaaactaag cagtggctct attgagatat tggagaagtt
     5221 aggatttggt ttaggagaaa tgcaggagga attcttcctg ctttcacaga ggccgtttag
     5281 gaaaatcaaa gttccaaaag tgttaagttg ggacttgacg ggacatccgg accagaatat
     5341 tgtaaggatc ggcacagagg aaagacgctt cgaactgtta gacgatgcac aaagacagaa
     5401 aatgatcaga atcgcttcgc atccacgtga cccgcccagt gctctaaaag atcaattgga
     5461 gatgatacgt gaattaaaag atcaaggcta tacaattaca ctatatcggg atcttataca
     5521 gaaattgcag gacgttccct atacgactat ctgatagggc acgcaacaat ttcatttgtt
     5581 aaaaagccca ctggcacttt aaatattcct cagggaatag aagaggaaat cgaagaaatg
     5641 gcaatagaaa tagtgttgga tttagaagta tcactatggg acacacgaag ccatcctagc
     5701 tacacgatat taacgtgaaa tatatcaaat taatacactc tgcaagggaa tccagaaacc
     5761 tcgttttcca taaagtatct atctgatact aaagccatcc caaagaattt ctggcataag
     5821 gcataagaga taataccata aggtttcatc taatttgact tatagtttcg ggagtaactg
     5881 tcgggacaga taaggaaaag aagatgaaga ataatataaa agaaacggca taggaatgtt
     5941 gatgatagtg atattgatat tgattttgat gatgacatcg gtccttctat cggaggaaaa
     6001 ggatgatact ttgctatacc gaaactagtt ttgagccgtg atatgggagt gaaattgttg
     6061 ataaattcga tagatccgct aactgatcaa gggcgtccaa tttatttgta ctagtagacg
     6121 tataacatga ccttctgtag acgttcttca tatcaaatta aagtgttaca agtataacac
     6181 attgtaaatt tgctactagg tcattgttgg tgacacttat agttgcataa acggcatacc
     6241 ttcattctaa taaccaatgc ttactataac gtatgactta caatttcctg tctatccgtc
     6301 tcttgtttcg tagccaaagg tactataaac aagagttgaa ataagaacgt cattaatata
     6361 acatgatgaa tatcgcaata attatcaagg ttaaaacaac gactaattac atacgttaat
     6421 cgtatgcaat catgtcgatg tcatagcaga aactagttca gctaaagtat gttatgatgt
     6481 gaattgccgg taattgtttt ttggatcaat aatttagttc agtcttaaat atgggttttt
     6541 tttgaataga gcattcaata ttgacttctc tcattttggg ttcaagcagt tattcttgat
     6601 gtgcagataa gacatccccg taaatagagc tcgtagattt catttgcgta cattctagca
     6661 tccctccatt aaagcccccc cgtatcgcgt atgacacctt tttgagataa gtctagtatc
     6721 tttgctgcgc ataacgcatg tttgtatgaa tcactagtgg aaccatagcc ttgtgagaaa
     6781 gttgcggaaa accctgcacg atattaataa caaccgtcca attacgggat caaaataagt
     6841 aagagttggt cgtcttttta ttgagcatca taaaactaaa ataaaataaa aaagaagaat
     6901 ttatccagca acagattggt tcttgagaag tgattcttct atgaattttt gatatgcttc
     6961 tttgggagct gcacctacgg ttcttgcgat ttcttttccg gctttgaata gtaatagaga
     7021 aggaatagag cggacaccat atctcattgc aatttcttga ttttcatcaa catttagttt
     7081 agctactttt attttatcgc tcatggtttg tgacagttgc tctactactg gaccaaccat
     7141 cctgcatggt ccgcaccatt cagcccaaaa atcaacgagg acaggtaaat ctgattttgt
     7201 tacgtctgtg tcccatgaat tgctcgtcac atgaattata tcgtatcacc taagtggcta
     7261 tagcatagta actacatata aattaattag ttgccgtctg aatagaaata acatatcgaa
     7321 attgctaata ttgtggctgt tttagaagcc atattgaagt aggtcttaat aatcttacgg
     7381 taacaagacg gctaaattat taactgaaca gtttgctaac ctgaacttca gcaacctccc
     7441 tgcgatatac ttgctcgaac tgtcttggtt ttgcatcttt gaacacatcc ttactacaat
     7501 atttcataga gtatgcagcc atgatatcaa gaataggttg caatgctaac cccttatcag
     7561 taagatagta ttttatcctc acaggttttt catgtgggta cactttacgt attatcaaac
     7621 catctttttc catttctctc aaccttgcag atagagtttt tggattactc tcttcaatag
     7681 agtttaacaa ttggttaaac ctgctctgct ttccatttat catatttctt agaatcagaa
     7741 ttgtaaattt cttgccaata attttgaatg tattgtttat aggacagcgt ttcatttcga
     7801 tattacattt tgacggattt attagttggc gagtagtagc cgttgctgca ttagtagtgt
     7861 ttgggtttgt cggcatatat caaccattat tacaacgtct aattttaaat agttaccctt
     7921 ctatagttag ttaggaaaat acttacaagt tggtaatctg ctatccataa cgtaacttca
     7981 ctgattatac gtaacaagta cactatacta gattagtgaa tcaccatcta ttagatctat
     8041 ttctttacga accacctagt tttgggtgct gtcttaagat ttaatctgaa ggaaaaattc
     8101 ttgtcaaagg tgtgcctata tggtgccgca tagagtttga ttctttttaa aactttgcag
     8161 atgaatatac cggttaatca ggtgttattg ctatatgtta taatgatgaa gagcataagc
     8221 aattgtagat cgatattaac ggaattgatg atcgtcatgg ttgtcattta tgaaacatag
     8281 gaagaacgga tcaagtaatt gtaatggtgt agtgatgtcc attaagcagt agggtattca
     8341 aaaaatggct ccgcacaatg gcgagaatat cgtgctaaaa acgactaatg ttaccctaaa
     8401 acccgtagct cttttgcgtc ggccttgaat cctatctttg catgagctcc atcacaatac
     8461 ggtttactgt cagatgcacc gcatctacac atcgcagcaa acacgttgcc atctaccact
     8521 atcaagttag gcccattctc agtgcattta atttcgactt ttgtcataat tcatacacga
     8581 cttgtaatgt atataaagaa ccctgttatc tatgacgaat aatcatttag gtgacttgtg
     8641 cgttttcgtg tatttcaaca gtcaaaagct gcggtgtaga ttaagtataa gaagattgaa
     8701 ttcttcttag tcggcgtttc ctcaagcgac ttctgaagat cgccctgcaa ttctaaaatt
     8761 atgccagata gattgcaatc tcaaaagtgc tgaacagtaa aaatcgactt accatacttc
     8821 ggctctgacg aacccagtat ttctaacacc taataccata tagaactgtg caatatgtgt
     8881 gcatgatctg ggaaagagga tttcactgta attaaaatag acataaggtc aagtcaaaca
     8941 taagcaatga gaaaaagaaa agagtccgat tactttgacg aggaaggaaa agaaggtgag
     9001 ggtagagact atgataaaga cttttctgga gccggagtaa cagaatttgg ccttgactta
     9061 aataccaatc aattaagaaa tctgtctcca gaagagctag aaaagtattt gcgtagagag
     9121 gctagaaggc ggcggaatag aagataagga cgatttatct acccacctat taatccatct
     9181 atcttgtata gcgagtatta acgaggcgtt ccttctaaat tacttctaaa ttggctgtta
     9241 accatcgcct gattctatgc ggttatggct acacattgtc gtacagannn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnca ggctacacat tgtcgtacag tgggactacg caaccgagaa cgataaaagt
     9421 taagttcata agtatctcaa tatgcaaatt tcaaggcctg ttttcaaaca cataagcaat
     9481 cattccagat ctatgaaaac aggtgaagac aatctatttc tatatctgta tttattataa
     9541 tctattgaat acaatagatt actacgaaac cttggtcaac caaagtgttc agatagaaga
     9601 caactttaga aatgcggcct atcaattact acaacctata gaaactacat ttatgcggtg
     9661 gtaacatttc agttgtagat aggtgaatca ttcccccaat atttttaatg tacttttacc
     9721 ttgtgttgta aggataacct tgctgtgtct gtctttgtac tccacttcta ctaatgaatt
     9781 atgaaccagc tacgcttatt gaattgagta gtcccctcag cttgctgtgt ctggctgcta
     9841 ttgagaggcc cttaccattc ctctttgaga gagactcgat tataggcggt tttggtctcc
     9901 tacagtgcat cctgtaccta tccatttatg caagggggtc aacactttcg gctctacgga
     9961 aacgatccag aaatgatatg atcattggtg ttattgtatc aaatttgcac tctatattca
    10021 taagtgggtt cttttatcat attaacatgt tgttaaaatg ttggaaaata aagttgcaat
    10081 agtcacggga gcaagtagcg gtatagggta tgctacttct ttagcactat caaaagctgg
    10141 tgtaagagta gcagtaggag ctaggcgcac agatagacta caagagctag aaaagcaaat
    10201 tatcaaaaat aatggaggag aaatttttat tcaaaaactt gatgttacca ccaaatcgga
    10261 ttgtgattca ttcgctgacg ctgtagttag aaaatgggga aaagttgata ttctaattaa
    10321 caacgctgga ctaatgccgc taagttattt cgtaaatagg aaagtggaag aatgggaacg
    10381 gatgattgat gtaaatatca aaggagtcct atactgcact tctgcagtaa ttccacatat
    10441 gttagataaa aaatcaggac atatagtgaa cgtctcttcg gttgcaggga gaataatatt
    10501 tgctggagga agtgtctact gtgcgacaaa acatgccatt acggcattta gcgaaggatt
    10561 aagaaaggaa ttaagcccaa ggtataacat acgagttaca tgcgtggaac ctggagcagt
    10621 ttcaacagaa ctattggaat caataacaga tgaatccatg gcaggattta tacaagcttc
    10681 taagaatatg gaaacattag ggtcagacga catagcaaat gcggtcttat atgcaataca
    10741 agcaccaaat catgttaatg tcaatgagat attaataagg cccacggcac aagaaaggta
    10801 atgagctctc attatctagc cagtcttgtc tgccgaataa ctttagactg atgacaggta
    10861 gacgaatctg aaaactagta tcttctataa tacggtaact gacgctcaga cctagaaatt
    10921 actttcaatg gtggtagtca ttatgccctt caggcttacg gagatgaagc aatagcattg
    10981 tacaaagaag tacacaagtc tagattattt ggcaaggtac cggctacatt ttgaccctga
    11041 cgatgaaatc attccattga atccaactac aactgaggtt atgattacca gctgtagata
    11101 agaaacttga gtctgacaat tcgagcatcg ttcagacaac aggcgggtga ctgcacggta
    11161 ttttctgcac tttatatcct tcttagaggc tgaatatgac accatccaaa acgtccatac
    11221 aaatttaatt gtatagaaca tcaccgaaac tattatgcaa aactccaaaa gtgtattaaa
    11281 gccaaggtta acacagtttg atatttaagt taggattggt agatcaaagt atgataaaat
    11341 gcagtatttg taaggtgatt tctaatggga tagaagaatt tgaaaaacat ttaatcggtc
    11401 aacattagat gtttgatggt aacgccaaaa tagttcatta tatagcatat ttggaacaac
    11461 gactaacggc attagaagat taaggctgac cttttaggta atcgataata caacacaaag
    11521 taaagcgctt ctgtcgttgc actcatccta aaagccttca caaaaacaag gtttgtatgg
    11581 cttgtatagc aactggacaa aaatgcccat gtggtcccaa agacaaaaca ggtggtatat
    11641 atcgtcttgc cgatatgatg tcggctgact tgtttttcgt tcccgatcaa atggtaagtg
    11701 ttgaaacatt agatataatg gacgaccgcc aataatgaca aggcaaggct acaggaacga
    11761 agacggtgaa atcgtagaga cttcacatat tatcaacgct gtatttcagg aacaatacaa
    11821 gcacggcttg ttttgcaatc agcaatgtca aaagtatatg ctccttctac ctatttctag
    11881 agatcagaag gctactcatt gggactgcct ttactggtat cgttagtgtc gctgtcatca
    11941 tacctagtat tatcgctatt actggtagga ttacgattgt tttgttaatc atcaatatga
    12001 ctggctgtta aggtacgctg ctgtagtcat ttcgctatcc cacaccacaa atactttgta
    12061 tcattttcag gtagttgtat ggctgtaaat acatcactct tgaacattgc aggattttgg
    12121 gtcttccttc tgaatactct gtaaagcaat aatgccgtca tatccgcatg ttccattatc
    12181 cgttattgta gtctgattat acgttggttt tatgggtaaa ttcacagtct tgttcatatt
    12241 aacattcaat acactaaatg catatgccga tggagctacc attaaaatta acgcaaagac
    12301 ggcgataata ccagctacaa aaatcagctt agacatttac tttatcccct cgcttgcaaa
    12361 tatgcgtgag ctagcttcac aagtgtttga ttgcttgcaa aagtaccgtc cttaatgtct
    12421 tgtatagctg agtcgtcgca gttcatcaaa tatatctgag gaaagatagt atcatatgta
    12481 tctgtccttt gtagaaatat ggtatttata caaactagaa gtccttcttg ttcaacaagt
    12541 ttatgtaatg taacgttatc gttatgtaat ttcgctgatt ctataggtat ctgaaagtta
    12601 tggttaaatt tgtctagtat ttccaaatag tcgtgttatg ttatttgttg gttgtgttgt
    12661 attagtcatt gcaaaccttt acaaaagtgg ttgcttcagc tgccaccgct aaaattaaat
    12721 acttctcact acctttatac tctgaaggtt ttggccttct ggtgggaaat tgaaatatta
    12781 actattttga cctaccaacc tcaaaaactt attgttttat aagtcttaat tcccgaccgc
    12841 aaaattgaca atatcttaca ttattataat taatcatatc tcccttcgtt ccctttacat
    12901 tcatctgtcc agtttcttgt ccttcagtaa aaggagtcaa agtaccgtcg tcatttacca
    12961 ttgcccccat gctctggcaa caaatgtcta ttgtgtaatc catgtaatac caattgccaa
    13021 ataagatata ttagcttaat gatctaccgt tttttcacag actttttact atctttcaaa
    13081 attgcagcgc ttgcttcctt aaggcgtttg tcaatctctt tattcgcgtt agactcttta
    13141 atgaatgatt ctttggcttt atcagaagcc tttaaactaa ccagattttt atctacatct
    13201 gaattaaact cctcccatat cttatcttct ctgtcctgtt tctcaaatat caattgcatc
    13261 atctcttaat tcctgattgc atcatccctc aatcatataa tttaatattt cttacagcgg
    13321 cattgttctc ggtcgtatct attgtacttg ttggtagtat aaagaaaaga ccgtcagagc
    13381 cacgaatcgt tttaaagtaa gcacagggct attgcagcgg atgatcggag agagctcata
    13441 tattatacta tgaccttctg aaacagtctt tattgttatt cctaatctcc aacgagaacg
    13501 acgggcgccc aaaattcatc tccgaatgat tcttatcttc agactttggg ctaaatttct
    13561 tatagttgtt gtaaagcata cttctatcaa gtccgattag aacttgtcaa taacaacggt
    13621 agcgacaata tctttctgtc taaatttctt gtagaaaaag atccgtacaa gtgtcacttc
    13681 ttccatcctt atcctcttct acgaaaccag ataagttatt cctgtatttg ctacagcagc
    13741 cgttgttact tttactgaat aatcgtgagg atttactttg atattttgga taccacctta
    13801 acttctttgt ttgtagttct tccatcaacc gaaggtctct ttcagatgaa atcaaaaaac
    13861 tcctccacgt aaaggtgata taacatttac gactaatcaa gattcttgac ataaattctt
    13921 caggttgcat ttagttgtat aaatacactc tcacgtacat aaaatacgtc tccttcctga
    13981 atagaatatc cacgcataat tacaccatct aataatacag tcattcgtca aaaatcaaat
    14041 aaagaatagt tatagactta gttatgatga agttattcta agttcgtata aaggatctct
    14101 ctggtatgac gagtacatgt acataagtac aagaggaaca tcgactatat tcgtcatcat
    14161 ggtagtagca gcaacaacac taatgctcct aacatcaaac acaatcacag gttcagtatt
    14221 cgctgcaaaa acaagtatat cccatcacag cggcaaaaac aatggcaaaa ctagcagcac
    14281 tggtagtgga aacagtagta gcatcagctc cactgacagc accaataata atacaccatc
    14341 gacaataagt aacgccaatc caaacatgga caatctctta gcatgtgagt cggctgcagg
    14401 acatggctct ggacagctca ctaaggccga agtcatgaat tgctatttgc aattctcccg
    14461 cggttccgct agttcacttt ccattatcgg gaatgccaat aattcaccag gcaatgctgg
    14521 cagtggtagt gggtcaggct cgacttcaac aacacatcat gcaggatnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 tcaacaacac atcatgcagg atctccgacc acacacacaa atagtcatca ccacagcgca
    14821 actaatccaa gcggcttaac gggtagtact acttcaagtg gctgattcct gaggacgggt
    14881 accacatagt agaattcatt tcattttctt tttcttttct gaatgattag tttaactaat
    14941 gattgaaatt agccatagta gcgtcactag tactaagagg tgagtattat agaatatcga
    15001 tagatagcca aacgagtagt tgtcaaagaa tacatatcgt tgaagaaacc ttcgccaaat
    15061 acctagccca agatttttat aacgacttca taggcctctg aaaaaaatgc tgaatgggcg
    15121 gtgattcgat aagcaagagt gaaaaatcat ttctcttctg taaacaagta tctaaccttt
    15181 aatattacat ggcacttgta ccactagcgt cttgagttta gctgtgatac tcgatgccga
    15241 ctcgaatgac ctagctttac ctgcctaaat gtataatgtt ttatcgtttt atccgggatg
    15301 cttcaggcct aggtagggtc ggcgaatcaa attaactaat agactaggat tatcaaatat
    15361 atggtggttg gtgtcttaat ttagtaaaca acgtttgtta taaatcataa ctgctgctta
    15421 ccgaagtaaa tgaacgagtt aggcaactag gaaaattagg caaaatagga gttgggaatg
    15481 gcgatactat catccacttc tgctttaggt aatgagcttc gtgaaaacag gatagctgct
    15541 aatttttgcg actatgctga ttctgtagat aaggacgtag ctatacaagc aaagatagaa
    15601 tgcgtaacta atgaggaaga aacaaaatat gatctggagc aattgagatt taaacgtaag
    15661 ataggatatt gcaagagcaa agtttcgcat atgtaccaga ataagggaac agtcactttt
    15721 tacattgagg tcaccaagac tcatgttaag attggacgta aaaaataaac ggggatgtgc
    15781 tcatcaatgt actaaatgcg ggatattgta taactgtaaa gatactgata gatgtgcagc
    15841 gtcctttttg catggtagat gctccatttg cgatgcatat ttactaaaaa gttaacatag
    15901 atctagccgg gtttaaggtc tcagtttgag tattctgtga gtgccacaga gacccacctt
    15961 attgtagctt gtagcacaat agata
//
LOCUS       DAOR01000323            4042 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_59513, whole genome shotgun sequence.
ACCESSION   DAOR01000323 DAOR01000000
VERSION     DAOR01000323.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_59513"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    1437..1588
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1242 a          717 c          696 g         1235 t
ORIGIN      
        1 ctgcataatt aatattatta tgactacaat actccctaca aataaaaggg ggattcgtac
       61 ttgttctaaa taatccagtt ataccttcga atcgaagtcc gttgttccaa gaggttccgg
      121 cctctttttc cagagctttt ccaacagacg ttcaaaatta tctcaattta aacaacaggt
      181 gtggatgacc aaaatactaa acactaaaac tttccttgtt cttttctatg catggtatta
      241 ctttgttttc aattatagat ttagcatccg aacctacatc gaaagacaat agtagataga
      301 atttgccttc ataatcacta ttactgagta aaattgggac tgtagcacga atcagctttt
      361 cgtatttccc tatcgaatac tgtagtctac ctgtatttgc ttcaaagtct tgccgggttg
      421 cagcacgaag aacggcttgt atggcatact gtgaagtctc atcccttgtc atcaacggaa
      481 ctaagccctg tctatatgtt gttgcttcta gattacccat cctgtttgct atgcctgcga
      541 acctgatgaa ctcgtctgta tctagtattt gtttgcatat gtcagaaaat atctgttcgg
      601 acaatgtgta tgtgtatgtc tccctatttc taatgaagct atttgcagtt atttgaatgt
      661 ctcttagacg gcctgtcttg agtaaaatca tgcacacata ttgtgatgaa gatgtgccaa
      721 aaataccaag tgatatctgc ttcaacaggc tcgtggagta ttcggtgata cagcagcgaa
      781 gcttgccatg ccaaaacctt ctgtctgtct gaagcaatcg tcacacatcc aaacactgtt
      841 aacatattaa ccaacattta cttggcctca cctctcaact cctgcgaatc agctacatgc
      901 gatccacatt tgatgcatct gggtaataaa tcgaccatac agagtataat tgtcaaatgt
      961 attattataa tttacatgat taaaacaaat agtgtccaga cttcaagtta ctctcacatt
     1021 ctcgatgctg atgacttcct aggatgttcg ttataacatt atagctcatg gaatggaaga
     1081 ttttaattat gaaaaggcat atccttgata gagtaatcca tcacacaata tgatgcatta
     1141 taacaaagac ttcagcgtgc aaaaagtgta acaacgtata tcttcaatac catcataacc
     1201 agtaaataca actattatca tataatacaa agatatgtct acatcagacc cttcatctga
     1261 tctgaccgat ggttatatat gtaaaatatg tgggcttcct ttcttaaccc tacaatctct
     1321 tgcagcacat atagattctg aacatccaga ggcagaacaa cctgaggtac tgtaatgtaa
     1381 agaacttaac aagaaaacat cggattgaat gaaatgtaac ttcgagtcat tatcagnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa atgtaacttc gagtcattag cagttaacct
     1621 tgaacatata caatcttctt caacttttcg tcataatgta atcttcacat ttcttctact
     1681 tttcaccagc atactgagag atcttcattc ttcttgttcc tcgatgagat tttttaataa
     1741 gtgattattg acctataact ctaatggtca tatggttcca ttaccttatg gcataggaat
     1801 agaattaata gaatcaataa tcgaacgaga ccgataaaag agagcgataa gcgaatcgct
     1861 tcaatgatgt ttagattaaa atataaacat gtattatatg gtgtgtcaca atttggatac
     1921 aatacgtcct tatagccgct taacctttta tcatactact tgaaaacgat cccaagacga
     1981 ttagtacgct acaacgtaaa taataactac ggccaatttt tagttaaaat tccgttacat
     2041 accgtaaaac ccttgaaata ttcctgcgag aaatgtgaca atatgtttag tgcatggaag
     2101 tatttataca cgcacaaggt tgagtgccat tcatattaat ttaaaatgaa taggtaagga
     2161 aagttaagtt aagaacttgc aaatctccaa ttgtaggcag aagataatca ttcattctta
     2221 tcttattgaa atgtcagtgt tacattaggg taatgtacat tctaacaccc ctataattag
     2281 gctctacagg ttggtcatct gagagcgtat gtttgtgcgg gatactactg atgatactga
     2341 tgttataagt cgagttggta agactacttg ttgagttgct agtatttgac aggctatgat
     2401 gatggtggtg tatgttagtc ttggatgcca ccaaagtgtt tgatgtcagg agcgatgatg
     2461 ttgtcatcgc tactgccaaa atggcagaga aagagcagtt ggaataaatc tcgagatata
     2521 agagattgta taaagattgg tgcaatagct agatcaatag ctcgtatgtc ctcgagcact
     2581 gtacttttca ttggtttagt gccgtctaag cgaacatcaa ataattatac aaaggatgta
     2641 acatcggata ttacagttgt caaggcggaa ctggggaaat aagcaataaa acctggaatg
     2701 tcttaaggta taaactagaa tctacgaaaa ttgtgattat actaatttgg gatgtccatt
     2761 gttggattat ggacggttat gctaaaagac gcctctagtg caagcaaata ttagaaagtt
     2821 cctctaaggc tagtatggag ctatttataa tatgttttac ctgcatgtat tagatatgaa
     2881 taataatgga aaatgataat catccaagct ataaagtatt attttgtgta gatacccttg
     2941 tctctaattc tttctctaat tctagcaaca ctgcttatca gttagaaggc agacaaattg
     3001 cgaataaaaa atctgagacc gtctgcaata gtcttctgct tctcgccatt aggtcacctc
     3061 ggtaaataat ttgctaacat tctagtatgc ttaatgcatt taaaaagaag gaatggttca
     3121 atgattaagg attttgtagg tggaggattc aggatccgct gtttttattg taagacgttt
     3181 aacattgttt atccacaaga cccagagtat agccatccat tgctccaacc ttgcctccgt
     3241 aaagatagta tcttatcatt gttcaaatgc attgcttgta gacagtggac aaagtactat
     3301 tgggacatag atcacaacct agataaccgt gattagagat ctcaattgag ttaatattca
     3361 aaaaattatt gttattatgt ccataagata atcttgataa gtacccgtta gcaaacatcg
     3421 gaatctataa atgacacgtc ttgtcgcata tttagagtat cccaaaaaat tagacataat
     3481 ctatttggca ttgagctaac tattcattat caagtctaat cacaaattgt gagaattgtg
     3541 gcagcaatta tgccgatata caacactaag cttgaaagtc tacaattcca aaacgaagaa
     3601 taggtaggta cgtagtatta atatgatatc gattactaac gttgattttc tccgagtcgc
     3661 agtttacaac tagcataatc tgtattgcct agttcgccga tacggcgcgt gttagggcag
     3721 gttgcacggg caatattaaa catatgcacg gaaaagggta aagctatttc tgcctacaaa
     3781 ccttaccgaa ttgtattact atagactaaa ccaggttccc ttgtcgagaa taactagcaa
     3841 tttaagggta ggctaaacgt aaatgttgta ccattgcctc ctggattatt ttcagcccat
     3901 attttgcccc cgtgagcttc cacaatactt tttgaaataa acaatcccaa acccgtccct
     3961 atttcagatt tcgtagcaaa tttcgcgaat agtcttggta atatttcagg gtgtatacca
     4021 gtacctgtat cttgtatgct ga
//
LOCUS       DAOR01000324           10557 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_43701, whole genome shotgun sequence.
ACCESSION   DAOR01000324 DAOR01000000
VERSION     DAOR01000324.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_43701"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5366..5653
                     /estimated_length=288
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8301..8458
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2975 a         2105 c         2113 g         2918 t
ORIGIN      
        1 ctacgatcag gtcttggccc tgacacatct gcgcctcgac gaatgtcgcg ccgtagtaca
       61 cacccgcacc ctcaaaccgc aacggctcat caactgaaag ccgtcggtac tccgcgccac
      121 tcgccacgat gaccgctcgc gatgtgacat gcgagccgtc gtcgaactcg atcacgtacg
      181 gtttgcggtt gcatgtgagc tgcgtcgcgg cttttgcaat gatgagctgc gccccgaatt
      241 tctgtgcctg cgtgtaggct ctgtctgcaa gctcctgtcc ggaaattccg gtcggaaagc
      301 cgagataatt ctcgattttc gaactcgacc cggcctggcc gccgggggag ttcatttcga
      361 ctatcacgac gtcaagccct tccgacgcac cgtagacggc tgccgccagt cccgatggcc
      421 cggcgccgac tatggcgaca tcacgtatat gcgtctggtc gatggcgtcg ttgaatccga
      481 gacagccagc aacctcgccg ttggtgggat tccgcaacac gaccgtcccg cggcaaatca
      541 acacagggac atcgtccaca cctacatgga atcggtcgag caattcctga gcgccggcgt
      601 cctgatcgag gtcgatgttg ctatacggat gtcggtttcg cgtcaggaat tccttgatgc
      661 gcagcgtccc tggtgagtgc attgatccga cgagcacgac gtcgccgatg ccgtagtcaa
      721 tcaggctgac ccgtcggagg aggtacgccc tgacaaagat ttcgctcagt tcgctgtcgg
      781 tctgtacgag cgccaataac tcgtcgcggc cgatttcgat gacttcccct ttttcgacgg
      841 cacgaagagt gacaagtgct cgacgacccg aaagcgtgct gacttcgccg ctgaactggc
      901 cggggagaag gtcgatcacg agttgttcgc cggctgccgt ctcccgaacg atctcgatgc
      961 gaccggcggt cacgacgaaa aaatgctccg cgtgctctcc agctctgaga agaacatcac
     1021 ctgactcgac tcgtcgcacc tttccgtggg aagcgacgcg ggcgatctga gccgcactca
     1081 aggttgggaa tgtttcatcg gcagccgcag gccgcggaag tggtgttcgc ggcggtgtgt
     1141 cggtcatgcg gttgtccgtt cgaagagtcc tccgacgact cttcccctta tattattcat
     1201 aaattctcag atatctatca acggtaaaag cttttagtga aagtatactg aatacattcg
     1261 atttcgctgg aagacgaact ataagcgcgg ctaggacctt tgctacatat acatttctac
     1321 acactttagt gaatgctata tgggctatga acaaaagaac tggtttaaga cgttgatggt
     1381 agtcttgata agctgggatc gttggtaata ttgtgctcta ttttaccact ttcaaagaat
     1441 tatgtaagtg tttgagactc tacttaattt tcatttgcat ccctgagccc gtatcaattc
     1501 acccttttgt gtggggggac ggctgacgat cattgtgcgt ctggccgaag agtttaatcc
     1561 acttttagtt gatagtgcaa tttcttttgc tgcagacaga caacctgccc aacatcgaag
     1621 tatcaggttc tttatttcga atccatcatt tacatctgtg aggaagctcc gtcctaattc
     1681 tgcgacaaaa ttcaatcttt gggccttctc cttatcggtt aagtcgtgaa gaagagagcc
     1741 tgcaataaga ggtgaatcaa tgcagtccac taatctggcc cgtttttgca cacccaacat
     1801 agatacgggt agagacggtg gtgggccaat tatatcggga ttggtattgg gatggaacaa
     1861 caactgcgac tctgcaagaa gtgtggtaat gctatcaaat ggattaagga tcctgtaaat
     1921 gataaatgga tacaattaga gtatgattgc gatcttgaat atgatagcct ggaagatgta
     1981 gacatgaatg aggtggccct atggagaaac aaatgtgttt ctgtattaaa atgcaaaaaa
     2041 ggctgcgata catggattta tttcaatcat aagcaaaaat ctgtatccgg aaaattaata
     2101 ccgacagaat tagcgacgca tgaaaatcat acttgcagtg tcaggcaaga atttttaaat
     2161 aattcccagc ttatcaaatg atatatcaaa tcacgaatct attagcaaac tgtgggttgt
     2221 tatgccgctc tttattgctt ttgcatttct atttattatt ttaagtactt taatactggt
     2281 tagtaataac agtggttttg gtactaccaa tcttgtgtat ggtcaaccag atcaaaagaa
     2341 cttcaatatt acaaattcac caaacataca aaatatagca ataaaaaaag ttcacgtagg
     2401 agatattgat attgcataca aggtttttgg tgaaggtgac cctttgctac ttatcagcgg
     2461 tagtggcctt gttatggatg cgtggcagcc atctatgcta agagacctct caagaaatca
     2521 tacagttatt atatttgata accgtggagt cgggaataca acagctggta ccaaatcatt
     2581 ttcaattcag caatttgcga atgatactgt tggtttacta gatgctctaa aaatacaaaa
     2641 agcagacgtt cttggatttt ctatggcttc ttttatagct caagaaatta ctcttttgca
     2701 cccagaaaag gttaaccggc tgatactcta tggtgcaaca tgtggtggag aagggaatat
     2761 accccaaaca ccacaagttg taaaaatttt atctgatctg gtaaacaatc gtacgcaaga
     2821 cccagaaaaa attttatctg tcacatttcc actagaatgg gttaaatcgc atccaaatag
     2881 aacgatccca gaatccaaag aaattgtaac ttctaatact ctaaagcaac agtttaatct
     2941 caatgaagca tggttagcaa caaactggag tggcgtttgt agtcagctta caaaaatatc
     3001 caaacctact ttggttataa ctggaactga agatgttgct gtacccgcag ctaattcctt
     3061 aattcttgca caaaaaatcc caggagcttg gcttgtgcag ataaagggag gaggacacgg
     3121 attgatgtat caatatccgg atattttcaa taaggtatta caaacatttc tttcgaccac
     3181 aacaaatcct ggttgacgaa attatcagat attttaagta tattatctgg agatgttaga
     3241 tttagactcg agtctgatat agttttagcc atcgcaattc ttgaagtatt ttggtgcggc
     3301 tgtcttgctc aaatgaaaga gaacagacaa gcgtatcatt ctgggactac tgacagtttc
     3361 agacaaatac ttattgatgc gcctgatact gggcgaagat atggtccaat agaacagata
     3421 cgtcgtacct ccgaattctg aatcagaatt tctaatcaac catactgttg agagatgata
     3481 tagagtacaa agttcagaag aaaaacagca atctatccag attgttggcc agcggcagca
     3541 gcggtattga ttttgacttt caaataatag caagtgtaac aagatcagat ccaactggat
     3601 aaacaaaaaa gttggtaggt gtaccctgtc tgtcgtgtct gtcgtaagta gtattacgtt
     3661 attatcttat acttgttaca gggagtattt cagcccaccc accgtggcct aagtcatgca
     3721 catatgatcc cgtaacttaa aaaatacaaa gtgaactatc aaggtctgat ttaacggagc
     3781 aatacataat ggcgctcccg tgctaattta acagtattcg tctaacatgt gcaaatttta
     3841 atagttcctt tcagatattt gttatgtata gtttggctcc tcaacggcaa ggacccagtt
     3901 ctcagaagag tcaacagacg acggtcttta ttagttatgc cagggaagac actgatgcag
     3961 caaaaaagct gtataaagag ctaaaggagg cgggtctaaa tccgtagcta gataagtaca
     4021 atcttcttcc aggacagaat tgggaaaatg aaattgaaga tgctattcga aagagcagat
     4081 atttcattcc attattttct tcaatatcta ttaaaaaaat agaactaaat ttggactgga
     4141 tgtcttcaaa aggtacctta gcgcagcctc ttccatccaa atttgtgttg tagaaaatcc
     4201 aataatgctt ttaattcatc gcttgcagta ggaacattct gccacgtcaa gaacaaattg
     4261 tttttctcta tcaaacaata attgttcatt aacaactgca atactcgtgg atctaggaac
     4321 tactaagcat catgacgacc ctggaaatag ttttgcgttg attggaaaat atggctgacc
     4381 accgaaatct aagccccatc ctgaagcaat aattccagat tttataatag gatagagtct
     4441 tgtctgatca tagcaagcgt gatggaagaa aggggatgca aacaattctg gaattccttg
     4501 gataaatcag acagaaacat attcgatgag atgacacaac aacaaccatt gatgacaacg
     4561 atcaactagt atttccctcg tgatcctgat aagatttttt atttttttcc tgaaatgttt
     4621 attaatttag agttactacg agggacatag tacctcgaaa tgcagcatat tcttaggtga
     4681 gatacttttc caaaattagc ctcacgaggg gatagatgta aaaaatatcg attgagtacg
     4741 gtactcaacg actacatata ttaaaagatt taatgaccaa aaaaggttta tatatttgaa
     4801 tttcaatttt tattgatgac ggtactcagt ttcttttgat gatggtactc aattaggata
     4861 attcttttta tataaactac gataacatat ctgtaataac taatagtgac taatttagaa
     4921 cgcccttaag catcggactg agactcgaat tttcatttgc atacctctga gcccgttgac
     4981 catttgattc cgaacaacca aatgaggcgt catatttatg tggagtgtca tccttaaaca
     5041 ttaaatggca aatatgtata ccgatcctat agcaaaaata actcccagct ttgttttgat
     5101 gttaaaatat gatcaatctg tcaattttct tggatgggct cgctttgcta taactcactc
     5161 actgattata acatatagct gatatcagat gagtttgttg gataggaaaa gatagcttgt
     5221 ttgatctctc cagctttgag tcccaatcgt atcgctatgg caaatatatt gataacttct
     5281 tcagcatgtg gacccaatat ttgcgcaccc aatatacgat cattatcttc ttcaatcaac
     5341 accttatatc ccgaatgatt ttcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnatcttct tgtagaccaa ctgatgctaa tgggggaatg gtaaatacca
     5701 cgctaggaac ccctctgtag ttaggtttga tgtgatttcc ttcaagcaga ttagtggcgg
     5761 caatctctcc ttcatatgta gcaataggag tcaacggtag ccctccactt tctgctgcat
     5821 cgccagctgc atagactgcg gggttggaaa cgctttgaag gtattcattc actttcacac
     5881 ccatcttgtc ctcgcgtcgt atgcctgctg cggctaattc gaggtcatca atttctggaa
     5941 ctcgacctgc cccgtggacg accatttctg tctctacctt atatttctca ccgtcagtag
     6001 tcgaggcatg tacaacaagt ccattgttca tgttgttgtt ggctttagaa gattctattg
     6061 catcaacctt tgtttgtaaa cgcacctcta tgcctagatc gcgagtcctt tgcaataaga
     6121 tatcaacaag ataaggatca aagttgttta gaggcctggt accacgatgc aaaactgtca
     6181 ccattgcacc agctctcgct gcaatatgtg caaactcaaa cgaaatatat ccacctccaa
     6241 caaatacaat atttgatggc agatcgttaa gctccaagaa ttgatcgctt ttgcatatat
     6301 tgtcttctcc aggaatattc agcttcattg gctgtgctcc tgttgctatc agtatatgct
     6361 ttccacttag tgtataagtg tcatccacaa taacggtttt ttcgccaatg aaccttgctc
     6421 ttccatgaaa tgcaacgatc ccagctttgg agaattgctc ttctctttct ttgggtactg
     6481 gttcggtgaa agatcgcttg aaatgcatca aatcaggcca gtgtatctta agcttacttg
     6541 gatcggtgat acccttgcct tccattcgtc tattccaatc tactacttcg gctgcgccta
     6601 caagtacctt cttgggatca caaccacgaa gagcgcatgt accgccaaac ggcctagaat
     6661 caactactgc aacactccat cctgctgaac gacatttcca agccactgtc gatgctgcta
     6721 ctcctgtccc aattactata agatcaaaga tctttgtatc agacataaac agcaatacaa
     6781 tatgataatt aataataagt tgtttttgtg ccgacattac cctttgccct ctatgtatat
     6841 atacgacata attcttgtac tttcttacaa gaaacaacaa gaggaactac aaaaagccat
     6901 gaatagaggt atttttggaa tacatcacgt tacagctata actagcgacg cacagagaaa
     6961 catagatttt tatacaaata atttgggact tcggtttgta aaactaacgg taaatcaaga
     7021 tgacccaact tcatatcatc tttattatgg cgatgagtta ggacgtccgg gtacaattct
     7081 tactttcttt cattggccag atgcacctag aggacatagg ggaactagcg aggtgattgc
     7141 gacatctttt ctgattcctg agaattccat aaactattgg atagatagat tgaagagcaa
     7201 acaaattgag tttcgtgggc cttacaaacg ttttgaagat gaacaagtaa taacactata
     7261 tgatccagat ggactagaat tagaacttgt agcacataaa tctgccgaag atagaactgt
     7321 caatgtatgg aaggaaggtc ccatacctgc taaacaggcc ataagaggtt ttcattctgt
     7381 aactctatca gaagaaggat acgaacgtac tgcctctgtt ttgactgatg aattgggatt
     7441 catgcccacc catcaagatg ggagtcgctt ccgttatgag attccgaatg gagatgctgc
     7501 tagtccggga gaagaatcaa gaggagccaa tattgttgat gttctttgtc taccgtataa
     7561 acaacaagcg gctataggga ctgggtcagt ccatcacgta gcatggcgta cgcctactga
     7621 tgaacaacaa atggtcttac gccgcaatat agtcagagct ggattaaatg caactcctgt
     7681 gatagaccga ttttactttc attctgtata tttccgtgaa cccggtggta tactttttga
     7741 aattgctacg aatccaccag gcttcatgat agacgaaaaa cttgaagacc taggtacaca
     7801 tctcgtgctt cctccttggc ttgaatctac gcgtaaggat cttgagaaag ttcttcctcg
     7861 aatacattta cccaagaaag ataaagagca agaagcttta aagaaacagc ctgataaaaa
     7921 ggagaattaa tgaggagaat ggacaagaaa acccaacttg ggttcaccca ccgctttatc
     7981 ccttcgaata aaagtgaagc aagaactacc actctccttt tgcttcatgg aacaggggga
     8041 actgaagaag atctcatccc ggtgggacgc gaaatagctc cgaacgcagc tatactcagt
     8101 cccagaggca aggtcttgga gaacggaatg cctcgtttct ttagacgact agctgaagga
     8161 gtttttgatc tagaggactt gaaatttcgt actcatgaac tggcagattt tgtagaggcg
     8221 gcatcgaaag tttatggctt tagcctgcag tacgtcgtag ctctaggtta ttcaaatggt
     8281 gcaaatatag cagcaagtga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     8461 ccaatagtgc cagatcacga aaccaagaat cttttcaacc tactaaaaaa agctggagta
     8521 gcagatatat cgataaattg gcaaaacagt ggacacgaat taattttaga agagatcaga
     8581 aaagccaaag attggctgtt acgcgtcctt tcttccaaga acagcttatt aagcgattcc
     8641 taaaatttca acttggatat ttcgtaacta ctcccttctg gcaaatcttc aaagtaagat
     8701 tatttgcagc attcatgcct ctccaaatcc tgcttcgatc ttgcgagttg gttgaaatgg
     8761 agaaagatct gatctttatc caggagatgg aatgtgccca gacggaatag aaataacttg
     8821 gcaagagctg ggaaaataat cgtcagttaa ttgtcgaaga cagataggtc cagtcaaccg
     8881 acacccagtg ggccttcctc agacagtcta taaaagaaaa acagtagatt gttgagctgt
     8941 ttgtaatatg tttcgcttgg tatgtgtttt tatcttcgta ttgttcatct ttatgtgctg
     9001 gccactactg ctagtgggct agtttgtgtg tggtcgcgct cgccatctgt attcaaatta
     9061 ggtcggttga gtgcggacac acacattaac gtaagacaac ctacgttccg gtaacagcat
     9121 cttctggaga tgtagctgcg gttactgttg tggtcgttgt tcaactatcg gtatttcttg
     9181 ttgctgccgg tgctagagac caggcgatat gttgatagtt accttatcta atggtacgtt
     9241 agttgttggc cacgatactg cctgtccttg cttagcatat cgtatgctaa acgttgaaga
     9301 gggcgataaa atcgttgccg aaagtgagtt agtaccgatt aaaatctaca tgtttttggc
     9361 agcttcacgc tcttgccttt cttctctgtt tattacagat taataattcg tgggctcgtt
     9421 tcttctcgtc attcatcgct cgctggtcta gctcaagatg tttttcttca actcgtactt
     9481 tattccttga atatcattca ttaagcccgc tttgctttgg tcagattctc caaaggatca
     9541 ctattagcat ccattttctc taaatatttg ctacgcacgg aatcggctgc tgtccttact
     9601 acagtactaa cttgctaact ttaataggta aaaggtggtt taccacattg caacgtgaat
     9661 agtcatactt taattattgg agattcagcc tccatgactg agattgattc tgggtcgatt
     9721 catttagtcg ttacttctcc accttattgg aatctaaaaa agtatggaca agaaaggtta
     9781 ggccccaatc agtcttatgc aaaatacttg tacgaaatcc aacgcattct taaagaggtg
     9841 aagagagtga tggcaccagg cagatttgtt gcaatcaatg ttggaactgc agtgtcaaat
     9901 aacggaatga aacctattaa tggcgacatc atgagattga tggaggatct caattttact
     9961 tttaagaaag agattatttg gattaagcca aaaggtacac agggtttgtg gcaacgcggc
    10021 gtaactaaat tcctcaaaaa tgagccatac ccttgtttct tcagtttgaa tataatgcac
    10081 gagtatatcc taatatttga aaatgaaggt caaatgcaga tatgccttaa gcctgaacac
    10141 agactaccag aagatttcat taagaaagtt tcatggagtg tctgggacat gggagtatca
    10201 tataccaaag gacatcctgc accatttcct gatgagctac cagatagatt gatacaatta
    10261 tacacacttc cagaggagac tgttcttgat ccgtttggag gtagcgggac aactatgaaa
    10321 gtagccagaa acttaaaccg aaattctatt gtttatgaga tcaatcctga atatattgag
    10381 ctgattaagc agaatgtaaa atgggaagaa acgcttgatc tagacagaaa atatgaaatt
    10441 aagatcagaa aacctattac aaactgaatt agcaaaaata aagcatcttt gtgctttaga
    10501 ttgggaatta ataactgctc acactgtata ctacacacat ggatatcatc catattc
//
LOCUS       DAOR01000325            7317 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_65644, whole genome shotgun sequence.
ACCESSION   DAOR01000325 DAOR01000000
VERSION     DAOR01000325.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_65644"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2353..2597
                     /estimated_length=245
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3415..3691
                     /estimated_length=277
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1864 a         1393 c         1256 g         2282 t
ORIGIN      
        1 gttttgatta cctccatttc ctccatttgc tatgccttgg ctgagatccg tagagccacc
       61 cctcccccca ttgccattga ttgaaaatcc agagttaagc gtattacccg taccaccagt
      121 ccctccatta gcggcactct tctgaacgaa ctgggatata tagaacgtga tctgcataac
      181 accaacgtta gagttactgg accaggtcgg gcaaattgtg ctaggggaat tgatattctt
      241 ccatcagata tccaaaagtt acgtaagcgt ttgggggagt aaagatattt aatatcaacg
      301 ttttccaata ccttatatct aaataagatt caactagtta tattaataca ctttaggata
      361 ctataataca tattataacg acgcgagttg ttaggttgtc gttactgtcg agatcataag
      421 tctgcttcgt attggtgcaa cggctacgct aaatacaatt tatgaattct ttcaatcaat
      481 aaacaatgat cacaggctac ctctgtggtc acataaacaa gatggttttc gcccaagggc
      541 atagtaatgc gtttaatctt ttcgtattct gccaatacat actttcctct tccgatcttt
      601 ggtgcaagct cagcgcgtat cttccatcct ttcaacgcca tttcaaggga ttttttgctc
      661 tcttttgaac tgagcagatt cctagtgcct tcgcggtgac cggagtacat tatttcgccc
      721 ttcaaatcag atatagttac aaccctaata ttttgatcac tattcaatat attttcataa
      781 atgtgcttaa aatcggtcca tgtatgatga ataagttttt tttgatgatc tgttgctttg
      841 cacttgtcag aacaataaca atttccgcaa caagaacact ctatcgcgta atcttgcgat
      901 tttgtgccgc aagttgcaca agtggtgctc atatccatgg tagtaataag gagcacctaa
      961 gataaaagga tccaaattca taaaccctgg gccagttacc gtaacctctt gatcgatacg
     1021 cgcattgcca gatatgttag gtagtggtga tactatatct gataaatcaa ggttgttaag
     1081 ggactgcagt gcgacagccg gatcaactga gtggtatgtg catatactgc gaaatccggt
     1141 tctgaaattc attcatttcg ttgcgagttg tatgacaaac aaacgaagac cccagatatt
     1201 tacataaact ctattattat ggattcaaga gccaaacagg ccaccggtca gtaatggaaa
     1261 gcctaccctc gtccagaagt tacagatatg gcaatggggc aagacagtac tttctcaacc
     1321 gtgttagtat agtttttgca gtcttcttgg tgacatcacg tagttacatt tcgatccatt
     1381 caattcaatt tataagagca tttcaaccct ccacgtcttc ctagatatat tagggaacgt
     1441 taggaaaatt gacgagattt ataagactat aagatcctcc gaattagtag gcaaataggc
     1501 atgggcggta gaaaaagaaa aacaatcaca aggccacagc gaaagactcg tagtactcaa
     1561 ggacgctgtc caaaatgtag tacaatagtc aaattcctag taactggtcc tgttgggaaa
     1621 gaaatctatg aatgtactgg gtgcatgaca aaatttccaa tagacgagtt ataatgagat
     1681 taagatagtg ttacggtggc atggccgcta cttgatacca cttagatctg ctaaccttct
     1741 tgtaatcacc cttctgggat tctcttactc tttatcttgt cacgcctaac aatttctttg
     1801 tcaatttctt cttgttttat tttctctccc ctgcgtcctg gggttttcct ttcctgttga
     1861 tttactttta ctctttcttc aaccaattcg tcattttctc tataactgtg agcgtcctgt
     1921 tgccttttct ttcgcaactc ttctgcatac ctttctctaa aagatctctc ttgatctgtg
     1981 tcattatcag acatgtaaat taaacctgaa ggacataatc aatacatcac atttttataa
     2041 atttttagtt acaagtattc tatgcctgtt tggaaatgca attagattat aatagctcgc
     2101 aagcaataca aatgcctcct aaagatagat atatttgaat atatcccacc taaacgcact
     2161 atatttttga aaatcgaaaa ctaaagcggt aaaaattagc gtccgtccta gcgatggcgt
     2221 tgctcgctgt ctttacaagg ccatgatgtt ccgttcttaa actcacactt actgattccc
     2281 tttgtgtgtg acatgttttt tatgagcttg cacagctggc tgttgttcct gttgttcctg
     2341 ttgtggctgt tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnctg ttgtggctgt tgttcctgtt gctgcaatgc tatcctatat
     2641 tcatctattt tcttccttgt tcgcaatttg gctttatatt tcaagtttcg tggcctcgtt
     2701 cttaatctat atccacaaca tgggcaccat aatccgtccc atttcaagaa gatttcgcat
     2761 acttgacatc gtttttgtcc tgtggaataa cggccagtcc ctactggttt catcgcttta
     2821 tggcgtatgc atataccttt gcaagtcatg tgattttttc ctgtaatctc cttgttcata
     2881 gttgagacat cctactattt cagattttgt taccttaaaa ctagttaaga gagaaaaaag
     2941 gtgacttggt ggctatgccc tcaacctagg cctatgaaag cttgattgtc cccctggtag
     3001 cagtggaagc aggctgtggt gaaaagatta cgttacactc agttttggga catattaggc
     3061 tgccgcgtca tcctgtttat ggaccaacag agttaggctg ggccagagcc gggtcatgga
     3121 atgacagtga ttaattacta tgaggcggta ataatctagt tatggacaac attcgcatct
     3181 gttttgtatt attaagaaat ttagctgttc tcatcgttaa catggggtgg tagcacaata
     3241 gtaattgtgg ctcgcccggc ctgcttgtta ttatatccac tatgcgcata tacaatcgat
     3301 ttagctatgt aaactcctag accggttgca cgtttattct catttccaaa tgttcttgta
     3361 acaaatctgc caaatagatt tggaaggata tcttttgcta taccatttcc actannnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctagtcttt tgttatcaac agaaacaagg
     3721 ctttctattc ttatggctcc actatctgtg tacttagcat tgtctacgat ataggatatt
     3781 gctcgagttg cacgactttt atctgcgtaa atttctacct ctttattatt atcattattg
     3841 tcatgaatac taccacttct accaccacca atattatcgc caagccttgt ctcaattgaa
     3901 acaccctctt taaaactaat tttcactacg tttactatat ccaaaattat ttcattgatt
     3961 ctaacttttt acattaccta ggtaagactg gtaccacttt caacgctgct aacgtctaga
     4021 agtctgcaag cttctaaaag tatgcttcta ttggtatgta ttgattatat tttctgtata
     4081 acgtattact tcaatccttt caggattagt atcagcaata gtgggatcgg caggaactga
     4141 caaatattat atcacggcta accccatatt tagcccttta tcgagttata tgaagctggc
     4201 tatcattcat tatatttctc catcctatgc aaaagccttt ctctggtgtt tcggattgtc
     4261 tttagcgaca ggaaatttat agcggttaaa aaacttagtc tatacaaggg ctggtagttc
     4321 aggggtagaa tgtgcgcttc gcaagtgcaa ggtcgtgggt tcaaatccca cccagtccac
     4381 tgtttccaag cgaaaaatta cggctggaat ttgaacacaa tttttggatt ttgctagcat
     4441 aatgtcgcaa atttgatctt gaaactgcat cttttgctag caaatatgct agcaaacagt
     4501 tgctagcatc acatggaata gtttttagtt gttagcaaag gcgaagaagg atgggaccag
     4561 gaggaggaac agatttcaag gcatttgttg cgagaccctg atgtctccca ataaacagca
     4621 attaggatta gcgttgttgg tccagatagt gcggtagtag tcgccctcag ggagttgtgc
     4681 ccagattcgg gagacttggc taccagttgt gaattcgcag cttccagact cgctatagtc
     4741 cgcagaccgg ccgcttggta ccttgccatc cagcactagc ttttttacaa ttagtagaag
     4801 tagtagttag tttttacaat ttttttacaa ttagtggtac cggtttttta aacttctata
     4861 attctgcgaa tactgaatta tcaaaaatct tgatgcagcc gctagtaacg ggtacatcat
     4921 tatttcctcc acgaatgtaa aaaaaatcac gttggacttt ttcacctgta ggggtttcaa
     4981 ctgtcttaga tccttgaatc ctgattcgaa ggcttcccca acaatccatc ggtgtgaaac
     5041 atttctgtgc aggatgatcg ctagtgggac aagaaacagt acaatagtga ttgggtggat
     5101 cagcacatgg agagttatcg gtacttcgaa tctcttgtat cccagattca attccgagtg
     5161 ctccacacgt cccctgctga agcttgttaa ctaccccgcg tacaatattt ggactaagga
     5221 gatacttgcc atcaggaatg ggtccaacat ccttttcata ttcttcacga ccagaattac
     5281 cgccctaaca cttttcacta attttccttt acgaaaaata agtaactggt ttccatcata
     5341 ggctacaaaa ttgggtgctg ggtgctcgaa ttctgtctcc gcatgacctt ctagtttagc
     5401 gggcggtgcg atttcaggag gtggtgtcgt tacccctcct ggaaggggct cgaccgcggc
     5461 atctgttagt gttcccgcat catattcctg tctctgtata agtggtacca ttgtatcata
     5521 ttgttggaga acatgggcca gctcgtgtgc taatagtttc tttccttcat tagtatttgg
     5581 ttggtattgt cctcgcccaa atgcaatatc gtttcctagg gtataagcta atgcatttat
     5641 tgattttgca gattcaatag cattatcatc gttatgtatt tttatattac taaaatcata
     5701 tccaaaacga gattccataa attctcttgt tgaggaatct atgggtgatc ctgatcgact
     5761 caaagtatta ctaatatctt ttgccgcgtt atctaagatt tcaaatccat tagatgaatt
     5821 attcgttgtg gtctctctca gtcttttgat atagttgtgc gcaatacgca gtatgtataa
     5881 catgacgcag cagattgcgt gtcatttaaa atgattctac taagttgatc atactgaata
     5941 aatgacaaaa taaatcatgc attaaattca ttccaacacg ttgtccaaaa catccgccat
     6001 ttctgaagtt tatttaattc atttttcatt gagctagttg gtgaagaatt atcctttttc
     6061 acatcatctt gatttagctt gtctaataga atatcagaga aattgccctc ttcgatatca
     6121 tattgcattg cttcaagtaa tgcttctcgc atatattctg aaacaggctg gtcgtaagtg
     6181 tcacaaataa ttttcaaaac ttcgaattga ttctttgtaa ggtttacttt ttcgttaatt
     6241 gtaaccatct tagcgggttc cttactgtaa gtttcgttaa attttgatga aggtaaagat
     6301 gaggacgggt tattcatctt tttcctgttt atgatatatt aacatcagct atatcttttt
     6361 tgtttggtac acccatagtt tccgtatttg ggaatattta tcctaaagag gacatggttt
     6421 cctttgtaac tactgatgga acattttctt catcattcta agaaggatct aaaccatcca
     6481 cttggaatcc ttacaacctg tcaaacacaa gaatagggtg ctattaagaa ccactatagt
     6541 atattatctg tgaaactgtg gacggtacaa ctattttgag attctgacca gtatcgggat
     6601 gcccccggca aagggaggta ttctccaatt cctaaaccct ttactacttc caaaagtacc
     6661 aacctcgaga attgccgttg aaatgtcagt tattgtgctt atccattaat gcaactcgtg
     6721 cattagacaa gatctcgttt agtctatcat tcacgtcgag tttacttcgc aaaacttcaa
     6781 tagcctttat tacatctttc tttatctctt gtattgaagt aggcacttca cctttgtagg
     6841 cttttgtcaa cgatacgaat tcaccgccgc caccaacaac attgttcaat tttaagtttt
     6901 gaaggtgttc aaactggacc ttcattatgt atgtaatata tgcggcgatt gaaaccaatc
     6961 ttaaaatttc aatttttaat gaattgaccg aaccatgcaa atagtcaaaa gtgtgtcgtg
     7021 aatcaactaa gctgaatatg atcctttgat tgtgtatgct tagttctcgc ttcttcgttt
     7081 gtaacgaacc caattccttt ctcaatctat ctgattcgta ggttaatttt tgtatagctg
     7141 atttaaggcc tccatacatt tctagttcag aagtaaatga ttgcctgtca ataccggcaa
     7201 catacttttc aaatattgct gctaggttaa caatatcttg ctcgtttact cccatttgag
     7261 ttagtctaac taatttggat ccaactaatg gtagtaataa tagctcagta cgtagcc
//
LOCUS       DAOR01000326            8388 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_51178, whole genome shotgun sequence.
ACCESSION   DAOR01000326 DAOR01000000
VERSION     DAOR01000326.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_51178"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2555 a         1649 c         1612 g         2572 t
ORIGIN      
        1 ttatctgttt catgagcctt gaagatgtgc catgaaataa aactgccggt ggttcagcgg
       61 gtactttgag caccttatcc ggtagcgaat gcccatataa agcacgaatt tttccttcgt
      121 atagttcaaa acgattcttt gcggacttgg agatcatttc aattatgtca ttttcggtaa
      181 gatgtcgcca atcattcctg cggttacgga aacatatgag taaatcagtt acacgaaccc
      241 attcatgatt atctaattca agtccataac gccaaggttc atgtcgaaga gcatgtgaaa
      301 gagtacggct caattttatc aaaaatggat ttcggttggc gttgctacct ttacgcaatg
      361 cttctcataa tatagcttac ccagtaatta ttaaaatatt atgatgcagc ctaaacgtgc
      421 attgtacaac gtgagccttt gataggctaa tcgaaaactt atttttcgct tccggaaatg
      481 aaagttgtaa tttcaatttg cataatttgt tgtttgttta tctttattca ttattgaatg
      541 agtgcatgcg ggaagatatt gaatgtttat tgttctgctg caaagtcggg ttctcatagc
      601 gactgctgca tgtagggtgt tattgctgca caaagggagt cgatggtaat ccgtaaatat
      661 ttggatgtcg aatggtagat gaaggcaaag acaacttaca gcatccaata gcaccttatg
      721 ggtagcaaat aaattgggtg ggatttgcgg taacgcttat tcgggagtcg gccttcattt
      781 ttattttggc tcactcatct aagaccgaaa ttattattag agctttttgc tgcgcgtcgg
      841 gagccacttg cctaaatagg ttagaatcgc cattagcata gaacattgct agagcacaaa
      901 acactagaag aattgaagag gctaggaaaa caacaattag aaataaaatt ggatatgttg
      961 gaaatactac tttcccttcc aacaatagac aagaaggaat ttgagaattg gtatagatca
     1021 catccatatt ataaaggaat atcagaagta gtcgacattg tcagaagagg agcggaagag
     1081 ccaaatggac aggctaaaaa ggaacgcaat catggcaagc gagccacgtg aagggattac
     1141 cgcagtagag gcattagaag aatttggtga agaggccgtt tcgagactca tggcaatcag
     1201 ctctagtctt gccgatacac atgtcaaaca agcagcaaat tctgcgattg caaagataaa
     1261 gcgaggttct atctaagccg ttttatttcg cttttatcct tgtcatagaa gcgttacgtt
     1321 tatagctaat ctgacacatg gatttcagtg tgcactggtg ggtttaaata tccaattgta
     1381 agcattaatc cttcgtttgg attattatca ggtactgact caaataggca atcaaaccct
     1441 gaaattgttc tcgcagccga tggctgcact ggtaacgggc atactgggcg ctctgataat
     1501 gcgtttttta ttgatccgta tgtagacaaa cgtaacgaaa gaagagacag gacgagaacg
     1561 catcataata acgttttaaa agtctttcaa aattggcgca taatacaact tcgtcgcgtt
     1621 gcaaccatag acggtcttta caagacttgc attccactat atttcgatgt ggtaagctcc
     1681 ttacactggt atgataaata caatgaaggc attgtacatc tgccaaatta tccggggccg
     1741 tatgatattg ttttgaatat tataagtcta gaaaatgaac ataatcgcac aacatctcaa
     1801 ttcatggctg aaagggaaaa agggattaaa aaagcaattt ctgataacga gaacatatca
     1861 attaaagaat ggaagacttc agatgacaca tacccgtggt ataaattggc aaaaattttt
     1921 gaacatcttc aaggcgaatc ctatcagcat atggagctta gaattgaacc gtatacggat
     1981 tatgggggaa agcactatta taagctattt agaaacccag ccgacttcat tgcggttgca
     2041 gaagagataa atctgcaaaa tctgagggat ctgattgatt cagtagcaaa aaccacggtt
     2101 agtagactaa gagaaatcac ggaaaatatt gataagatgc aaaatttcct taatggtttt
     2161 agtgaaggca tagacaatat attacatcaa gtatcaacgc gtggattaaa aggacgatgc
     2221 catgaagaac aagaattacg tgttcctgcg atactcaggg gtcgggtcat gcgatttttc
     2281 cgacgtcgac tattacgaaa tcctcctgtt tgagctgccc ctgtttttat tttgttctag
     2341 tccaacagct ctccttgccg tctccaaacc acttaaaatc ctgaatcctt agaatatcat
     2401 taatttcgcg ccatgttttc ttgtcgatcc attctttcgg tttgattttc acacttgcat
     2461 tttcatgtat aacatcaatt ttgtttgcgc gaattcccaa agcggtgaca aacatttctg
     2521 gtttcttttt caaagctgct tgccgcatca aatcttcgac tctgttttct cctaaattca
     2581 aattgatgcc agcgacttca gcgggcgttt gtccttttag cgcctgatgt ggccttatga
     2641 aattgtaatg aatcctcata gcgtctaaca attcctgcgc tgcatctgca ttatccaaac
     2701 cgcgcatcac ggtttctcta tcacgcattg ttccgttcaa tcgttcgact ttagaattca
     2761 atccttgatg tccgatgcta atgcttctga cattttgaat ccttggggtt ttcaatgtga
     2821 acaattcttt ttgataggct tcatcatata ctcgcaatcc gtcatggacg aatgcttttg
     2881 gtaaaggcgc gcgttgtttc atttctttta gtaatgctac acccgcttga atgtcgcgcc
     2941 tttggctgag cctagagcat atccagaacc gcgttgtcga atccatcaga ttccatagcc
     3001 atgaatagtg gttatcaaag aatttgtggg tgtgattctc ttcgttgtca ttcatcgtct
     3061 gttcgttatt ttcttttcta acatgtagta acatttcgtc aacgtggtaa acgccccccg
     3121 tttttggaac aaggccgtca acatacggtt gtactagtcg ggtaaatttc ctaatccatc
     3181 tacagatgct tgaatcatcg atagagaaat tgtagagctg ttttagatgc tctgctgttt
     3241 ttcttgttga tacgcctttg aaatacaaat caattgctgc ggttataatt ttcgcagaag
     3301 ccttacaatt ttcaaaagcg ggatttacaa tgaaacggta atcacagtct ttgcacagat
     3361 accgttgaat gtcgccgctg tccttgttat gtctgattcc aaatttcttg taattcgggc
     3421 taccacattt ttggtagatg atttctccta gcttactttc tgtagcgggt atatttgcaa
     3481 atgtatcgaa atagattttc ttgcgtaata atttgctaaa tttgacggca tgaacgtgtt
     3541 tgcattcaaa atgtctgaac gtaaaatcag gacaatcaca tgtccacccg cttgaataat
     3601 ccttggcaac ggtataccac ttatcattgt ttgattgtga tttgaccttg taagcgagct
     3661 tgtttagcct ctgaatactt acgtctggat tgtttcgtct accgattgta tttgctatcg
     3721 ccattcctcg catttcacgt gttgttacca ctttgctaca ttgctatcta tctacattgc
     3781 cacatttaag ccttgtggtt atgtagcaat gttcttataa tgtgaagtag caatgtagat
     3841 tagtggcgat agatgggtaa aatcaatcta acaattaacg acgagctaga aagggaattc
     3901 cgtgagatgg catataaagt caaaggcatg aaaaaaggtt ttctgacaga cgcgacagaa
     3961 gaagcaataa aagtgtggct aaaaagtgtc aagtcaaaag agaccagtcg ctgatatgtt
     4021 cgctatttga ataagcctaa tattgcttcg atttttgctg tgtcgtgatt atccttcttc
     4081 attagtgtgc ttagacgaat accgcgttct cgaattttta tcatctgatc gtcacctatg
     4141 tctttcctga ttctggttaa cgacgttaga tcggaggcaa tcctttctat tatgttatta
     4201 acataatttg ggctttcatt tttccagctt ggaacctcgg atatatattt ttcaagcatg
     4261 gctgttttct cgtcaatatg gctcaatgtt attcttctca gaaaatctaa catatcggta
     4321 aattcattac cgcgctttct gtaagtccaa taatatggaa ttcctagacc aaagcccaca
     4381 ctaccaatga ccccggcaac ggagatcaag ccccagaata ccccttcgtc gaacataaat
     4441 atggtaatgg gaagtactac gctcaatata acaataatta ggcttctctg tgatacgatt
     4501 tgaggcatac gttagaagca cccgatatgc agcgaaagca cccgtgttgc agcagacatt
     4561 tttaacaccc tcattcgcag cagagccatg tttattcaat aataataaca tcactagcga
     4621 ctattaggtc gcagcgccca aatgttttgg ctcgcgtgca taaaaacctc ggtgggctgg
     4681 actggttttg ttctccccgt ataacctaaa cttggctcta ggacataaac aaacagtcaa
     4741 aattcggatt gagaacaggg gcatgctctt cgttatcata cctaagagaa caatggaaac
     4801 agatacagtc agagatggtt gaatcacgtt tttgagccat tcaaaatttt gtcgagggat
     4861 ttatttacac attaattcat gttgctgggg ttatttaccg acgtgatatt cgaagccaaa
     4921 aagatttcgg ggcaacaaca actcaaatgt agtaattata tttattatat actactgatt
     4981 catgcaggtt gcgactagtt aactaatgaa aaattgtatc gagcgttgat tttcgtttat
     5041 caaccactaa gattaataat gtttcctaat gcggaattat tctcctctat aggtagaatt
     5101 aatgcttgac gcgaaataca aatcccctat ccagacacaa attggtttgg gatcttgtca
     5161 tagtcgctat acgcaattcg tattccgaaa tacgtctaga taccatagcc aaaggattac
     5221 gttgaagaga cgtggtgtat gctccttacc taaaatagac caagactgaa accaagttcc
     5281 ctaatctaga ggagaaaaat attggccgac ttcatgtgga aacccctaga acagtagaag
     5341 atttaagaga aatatcagat cagtacaaac aaataatcaa accattgtac gattacacta
     5401 caaaacatag tcaaaagtta ttcctctatt ctgcatatgg aaagggtttt ggtaatccga
     5461 aattgcctat taaaggttcg tatcaaatga gcagcgctgt tgttggcagg aacgatgaat
     5521 ttaagaggag caaaagaccc agagttacta ctaccgacaa taccatttac ccatatttgg
     5581 aagtaagtat ctctattaag aattcaaagg cactgttagt acattacaag agattatatg
     5641 aaatccagaa taattctgat gaaccaattc acgaagttat acacggcata gcaacagatg
     5701 ttgaaaaaac atttgatgat ctcaatatta aagtctatga tgaggagaat aaaccactac
     5761 agatatctag tatacctatt gataagccat ttcagaagga gtttacgacc ctctttaaca
     5821 agccaataat aaaatcggat aaagagcgtc gatgttatgt tttagaatac gatgttgaag
     5881 agcaaggtaa gtactttgaa aattattttg gtaccaactg cggaaggttt gtagttacaa
     5941 tcgagtatcc tgcctatatg agtccacctg tagtttatga agtaaatcta gagactgatg
     6001 aggtgataaa gtgtaaaata caacccactg caaaaaaata aaagtaaata atagcagtaa
     6061 tactaaacgt agtatagcaa gatgggtgaa gagagaaatt atcaaaggcc aatcatttag
     6121 gtttgaatgg aaagagaggg tgcattaaga cttgcgacgc aaatgatgtg tattcgaaaa
     6181 ataaagcgaa tgagtaccag caaaaaaagc gggccttcta atatctgacc attatcgtga
     6241 atttccaact attttttatc ttgagatagt tgataggagc aatttcttcc attttgttct
     6301 gcaaccgcaa taatcgtatg cctttctctg ttactcttac tctgtaacag cgctttccta
     6361 cttcgtaatc taataggcca ctttcctgca aagctctcaa gtattctcta agctgattaa
     6421 aggatatgta ttatctctta tattacttgg tgacactcgt aatgccatac cgcaacaagg
     6481 acagaacaca tccttgtggt aataataaac ctcacacctt ctacagtatt tcttcccgcc
     6541 ttcataatgg ctcttgtcaa caataatttt tgaatacaac ctctcacgta gatttctgca
     6601 aaccatactg atttgtaagg aacgttggac tactaaaaat atgggagcta cttacagagt
     6661 gggagggtag acggcaacaa gaagaatctc atgacctaca acatttatga atcaaaaacc
     6721 gtcatcggtt cgtagaagaa gagaagctta cctattctaa ttcagctctt actcttctta
     6781 tcaaatcatc tacatcaact cgtttcgtaa taaatatctc ctgtatcgct tagtattgtc
     6841 gttgtatcga gtagttgtcg ataaatcatg tagcataaaa ttattctgtt tgtactctta
     6901 tgggtcaagg actgccttat gaagaaaatg ctgaatctcg tgaaaccttg cgaacttcta
     6961 cttctccaag acactcgtag tttataccgt cattcttcat tcatcaatct tcctaatagt
     7021 agtcttaaat gctgattttg tatgacaact tgtataatac ccataattag tgaatcaata
     7081 atgacacttg gtttggttgt gggagcatcc atgacacaca acggaatctc ccacaaaaaa
     7141 cgatgatcgc acgggagcgt ggagtattag ttgcagctgt cgaccttgaa tcaggcatgt
     7201 ggaaagtagc acgtgagatg ttcctaatac tgcaaaagga attgaatgtg tctaagaaag
     7261 aaatcgacga aataaatatt gatgcaatta aggacgtctt ggcgaagatt ggccaaatga
     7321 taaatatgct ggatggcgtt aggggtaaaa atacaaaggt tcgatccata tcgaaagaaa
     7381 ttgacgatga tctaattgac ctaaaggatt tggtaaagga gtaccaaaag gaactaagat
     7441 cagcaattac aggagacact aacatgaaca gctatgtaag ctcgaagcag tatcaaccaa
     7501 ctatgagcaa tggaaacggt aaggagaaac catcacaaaa actcttagga tttactggtg
     7561 gtcaaagtta attgagtctc taaaggagga gaagatcaac gttaccacca cgccatcctc
     7621 atctttattt tttcaagact cacacttaac tttgcagaac cctttcttca tttgagtatt
     7681 tgctatgaga gactaccttc tgtaaaagta atctggtact actcctggta ctgctactac
     7741 caatttgttt caagcatacg gttttcctta tatcttcaag gcggattaaa acacatgctg
     7801 cctattgcat cagtgcagcc ttggtgagta tgaccgccat taattgctac accgcctccc
     7861 tgtccatttc cattaccatt acctgaattg ccattggcaa cactaccaac tccgattgca
     7921 atgccaccat ttcctgcatt accattgcca ctagtactac cagacgctcc atcaccaaca
     7981 ccaccgttta atgctatacc cctattattc ctatataccc taccgacgtg cttgtttcta
     8041 tctttcaggt tgttgcaatt ttgatcaagt ccgattcgat gctccttcac accatctacg
     8101 tcttcaaagc tgtcttttaa agaagtctgt aattctcatt tgttctttcg tattcttata
     8161 gattttacta tttctaatcc attcattttt agatatcgat agattaacac atgcgaacga
     8221 caatgcattg tggaaatcgt caaaccgttt tgcttctcca tcaagtgctt ttcttattcc
     8281 ttctcttatc tgccatactc ctagtggcat aacatattct ggacgaattt ctcttagaat
     8341 caacgccgct gctttatgtc ttcttttgaa aagatgttca gccacacc
//
LOCUS       DAOR01000327            3631 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_42499, whole genome shotgun sequence.
ACCESSION   DAOR01000327 DAOR01000000
VERSION     DAOR01000327.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_42499"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3113..3215
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1064 a          658 c          631 g         1175 t
ORIGIN      
        1 ataagtgttt ctgctaatgc cttccagcga ggccaaagtt ttgtatcaat cattttagct
       61 ttataaagat agaacaagaa ttcaattaaa tcaaggtacg caactacagt ataatactca
      121 tcaggactat caaccaagtc taaccgttct gcaaataatt ggttttcttt tgccattttt
      181 gtaaatgctt cagtattttc cagtttcatc cttgtctcta ttactcgagc gtatatctgc
      241 tgttcacttt gtatacgcat acttctcttt actgaggaaa attgcagcaa cgacatgtca
      301 aacaatgctg caagtatttc aatagtaatt attggactaa tttctgaaaa gttcatatca
      361 ttttctaata ataagtaatt gtacgtttaa tttatcttca tggtcttgag catctatggt
      421 tctcaaacct tatgaatgtg atttttggtt gaaacaaaat tgaatggaga tttattccgt
      481 tcaattttga aattcataga taagctaatg tagttttgga cagtagtata ttagagttat
      541 acaatgaatc aattaaaaat cagagacgca cgaaaattac aatttgagac agttctagta
      601 atgcaggggt ggaggatctt tgggcgcata tgaatgtggg gtatataaaa cactggctaa
      661 gcgaggcata aaatttgata ctatagcagg cacttctatc gtggccatta atgcagcaat
      721 aatagcaggt tcaaagaatg atgctactag gtagttggtc tcctaatgaa ttataggtgt
      781 tccttgctcg ccgttctttt caaattgaaa cacgagtatc gtatttgata gatttataat
      841 atcaatgctt attccgagca catcattaaa aattatttga agcagactct taataagcaa
      901 ggcccaattc tctccgagat tgtgctttac agtatatcta tgtgtgctat cattattttc
      961 tactgtatgg ttaatttcaa taaatgcctt tttcattaga gtttcaaacc aagataaaaa
     1021 tgaatccaaa tctatttttc ctttcatcac taaaaggata tcctggactg cggtcttggc
     1081 aatatttttc gcaaaatttg taatctgttc tttactcata attgcaagaa gttcagccac
     1141 cacaggttta gctaaaggca ttgttccggc tcgaggttcg aacatgtccc attgcacata
     1201 tctttttagg attttattaa cagaagtgtt taagcttaca tcgtgcctaa tagattcggc
     1261 acgtaatttg tctatagtgt ctgcatcgat gcgaaatgta atagtagttg ttactttcct
     1321 tgttctttta ctccttgttc ttttactcgt gttattgtga tgccttatat tattaacgag
     1381 caaatggaat cgaagtgaaa ttcacctatt actaatatag atttattgtt ggtaggacct
     1441 aaagctgtaa ggcatacatg ttgtaacgaa ggatttagat tgtattatct tgacaattaa
     1501 aaaagagatg cacagtatca tttacgttgg tttcatcggc actttctact ttccatgagt
     1561 ttatcacgtc taagtgtatc ttatatattc tgaatattta ttatggggct atgactttga
     1621 tcttccataa cttgttttga gggatttcca tatagttgtg aattcgcgta aggattcttt
     1681 ctttataagt aggtatatgt tcattcgata aaacacattt aattgaatct tgtcggccca
     1741 gtcattagat ctttggatta cttgtgtcat cttagcttca agatcgtcct gcccgtaatc
     1801 ataactcgtc tcttctagct tgcaactcct tggcagttgt tgtccagttt cagcctcaac
     1861 attctctagg cttagatacc attctctcgt attaatatca ttatatgtag gacaaggctg
     1921 aagtacgtct aagaatgctt tccatttcaa gaattctcgc cattttattc taatatagtt
     1981 agaacacttt cttagaatgt aattagctca ctctcacgta acaacagaag cgccacggta
     2041 gaaaaaataa agtaaaaagg ttgcttatta atgtgcgtga ggtggtggtg cactgccctt
     2101 ggatagagat tcattgaacg ttccagcggc tttttcgtga aattccaagt tagattggtc
     2161 tactttgatt gcctgagttg gacataccga gacacaagcc atacaccaga tacaatcatg
     2221 ttctcttatt ggatcagatt tatcagtata atctttcctc tcttctttta ttgtgctacc
     2281 agttccagcg cttattgcat tagccatctc tattgcaggt acatcggttt cagttctata
     2341 ccactgaaat acttgtacgg gacagacttc gatacaagca ccatctgcta tacaggagtc
     2401 gaagtcaagt gccaccatag taccatggac ccctagtgga acaatttctt ctcctcttgc
     2461 tgcataagct gactttacat tctcgtcttc tgcagcctcg gctgctcttc ctggacccca
     2521 aacaaagtgg aagtgctcgc catcagaatg catatgctta cctattactt gttgattttt
     2581 tggaaattct ggttcaattg gcatgattta gatttgaata tgtaggtatt aataatgatt
     2641 gatctaattg caattatctg caatcgaaaa ggtctgggat ccgctaaaca cttaaaaact
     2701 atactgcgct gttatggatg atgtcctctt catcaccgtc atctaataat ccaaatagta
     2761 gcagacatgc atatgatcag caaataccta cagaacaaaa taaagaacaa acacaaaata
     2821 tgagaggaac aagaccaagt agcaggagta agaagagacg atgagattgt tacaaacttt
     2881 ccagaagctg cagcaatggg aagaattctg atgaacgtgg gatttccagc agacaagaac
     2941 aaaataatac aatttgtaca aaaacaacaa gaatccaatc cagaatgcag atttgattgc
     3001 aaagagatat tgcccttact tcaaaaaatt gaagaaagac attatgaaaa tgcttttgaa
     3061 gttacaaggg cggcaggact tgttcgctct gtcagataga tctcccatta tgnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatta tgactgaacg tgaaagctat
     3241 tagccgcaat tactaatcat aaaatttgaa aaggcgcgtg tgtcacggcg acttctctgt
     3301 tgccatcact aaattcttca tagaacaatg tatactcagt gcactcattt cataagtaaa
     3361 acagcagatg ttgtatccaa aatcttgaat aagagtactg ctgttttatc ggcccgttgt
     3421 agtagtagct cctttccatg ccataacttc atgagggtca gctatctgtc taaatacaaa
     3481 ctgcttctct tccttgatac aacgtgccat gtcctgtaaa aagtttaaac gcaatacgac
     3541 ctgaagccgc tatacttttg aaagtggata tactctaatt tgcttatgct atttacatca
     3601 ttgtgcgcta catcgaatct gcttttagcg c
//
LOCUS       DAOR01000328           12902 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_14813, whole genome shotgun sequence.
ACCESSION   DAOR01000328 DAOR01000000
VERSION     DAOR01000328.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_14813"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    446..672
                     /estimated_length=227
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2594..2783
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6219..6454
                     /estimated_length=236
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3653 a         2403 c         2550 g         3643 t
ORIGIN      
        1 aataggttaa aattaaaatt tagaaagtac agttaattta ttttataaaa tatcctagcc
       61 actctcttta aatggaagaa tcatataatc actcatgatc gatttagcgt ttgacagttg
      121 cgacgccaga tgctagcaat agtaccagga ttgaggtact gcagaatacc gaaaatatag
      181 ttgacgcata tttacaaatt tttcgtaatt caaagagcaa gtgggattat tatgcagatg
      241 tgaagtcggt aattttcgcg attgacatga tagaaaaagc tttgattgat accaaagcca
      301 gaggaataaa atcaaggttc attactgaaa tcacgaaaga caacttccat cattgcaaag
      361 aggtgataat gaaaattggt gaggttcggc acctcgatgg agttaaagga aacttcggtg
      421 ttagtgacac agagtatatt actacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nncaacagca acaccaacac caacaccagc aacaacagca acaccaacaa
      721 cagcattgcc ctcacatgcc gtatacagca atgtcaaaga agatatacag cagcatcaat
      781 atctgtttga taccctttgg agtaaaggaa caccagcaga acagaagatt agagagatag
      841 aagaagtaac agtacactat gaaaccaaga tattagataa tccagaagag attgtcaatg
      901 aaattggtcg tcttacggcc agttcaaatg agttatctaa ttgtcttaca cctggggatg
      961 cagtatagct ataatcattt ttttgagata aagaagaaac tactggataa gcaaaagaaa
     1021 ggagggcaca agggtataag atacataagc aatattaaca aagataatgc gaaattagcc
     1081 agaatatttc tagacgcaga ccgacataga gttgttcagg ttgttactaa cttactaagt
     1141 aacgctgtta aattcacgaa gggacacggt ggaactgtat ctatcagtac cgagaagaaa
     1201 aaaggcaatg acggtgataa ccgagaggtt gctgtagtgt gtgtaaagga taacggtagt
     1261 ggaatagact gtgacagatt tccaagatta tttgaaaaat ttgcaacgaa atcatttcaa
     1321 ggaacaggat tggggttgtt tatctgcaaa ggtattgtta atgctcatca tggcaagata
     1381 tggactgaaa ataatgcaga gtccggtgaa ggtaaaggag ctacatttta ctttactcta
     1441 ccaatcgtca atatcagccc ggagcgataa ccaaaaaata ttaacaaaat gtgctgaaag
     1501 atagacaggt caaaacaccc taaagtagtc atgacacatc tgggtgttca tttagatttt
     1561 aagacactgc tatctagctc tgatttggct agtccgaaga gctaccatca tcgtagatta
     1621 atctcatctc atcgaaggtt aacttttccc cgcttgacat cttctttaaa gcagcttcct
     1681 tcattatatc ccatctttct tggttttccc tatttctcct tgccctttcc tgtaagctct
     1741 cgcgtctctc ctcattgtca cgcggttgac gtcgttgata ttgttgtctt ctctgacgat
     1801 tttcggcagc ctgttgtcgc gattttctaa attgaattgc attgagctga gtctctacca
     1861 tttccagttt cgctttggcc tcccttactg cacgaattat tgtcctccta tcctgataaa
     1921 aactctcacg caaattcaaa agtgtatcaa ggttggcttt aagattacca attttattac
     1981 caaaatcaga ccgttggtcg aaaattttat tcattttcgt tcttaagcct tcatattgtg
     2041 atgacatgtt cctataatca tcttcttttt tgtgaagtgc gttgagtgaa tgcagcttgg
     2101 ttgcgatttc ttttgatctg gcaactaatt tgcgttcctc atctttcgtc aacttcttag
     2161 tctggatgtc gcgttcgatc tgctccaggt ttttcgtcaa ttttgacatg tcatatctat
     2221 ctctgcgtga tttcactgct gctgcagagc cacctttgtc gtccatcgac ttcattcgat
     2281 ttctggcttc agtgagggta gttttcattt cagtatatcg agttaattcc gtgtcctttt
     2341 gttcatttgt ttgcttcagt ttaacccgga actcctctag atggactctt tcatcgtcta
     2401 tctgcttttt agcggattcg atgctattgt tcagctcctg aattcgttcc tccccctttt
     2461 tctgttcttc taacaaggcc ttcttgaagt cgataagtgt cgtaagattc gaatctaatc
     2521 cagaatgatg atcgcgctca agattctttc ttggagttgt agtggcagtc gtcgtagccg
     2581 cagccgcagc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnagccgca gccgcagccg ctgcttttgc tgcggtcgcc
     2821 gatgtgcctc cattgtcggt actatattgg tccagtccca cgtcgaatgt agtttcttgt
     2881 attaccgagg atatattttt tctcatttgg agtagtggct tttagcctga cgccttgccc
     2941 aaagatgcat cctatataca tgtttatatg acatgagatt ctagtactcg taggagatca
     3001 ctagatggag gatttcgaga aggattttcc tgatttcgac tggaaaaata ttcctgccta
     3061 taagcatcct ggtggcaagg attgtccatg tcccaaacac gagtatattc gagagcagat
     3121 caatattacc aagactgtaa gcaataacaa tcgagaggtg agcccagaaa ccaggcagaa
     3181 ggcgcaagtt accctcaagc tgtgtcctga tcattacaat gtatattttc acgaagttat
     3241 tcctgcgatg ccctttaaat atagagtaat gacaaaaatc gcactcaaga tcggcgcagt
     3301 ggttattcaa aaaattccgt atatgcaatc tgaccaatgt ttctactgca aattcgggtc
     3361 cggtggtttt gatagaaaaa ctgaattgcc accaatttag ttttttttgt gttgcagttc
     3421 tttttcttct tttttctact tctttgtatt gaggcctttc gccatatttt ggagcatcgc
     3481 agattgatct gacgcaaaac ctagcgaaag tatgttgcgt aagagtatat gcaaatacgg
     3541 aaaattgcgt ctaactactg acgtgtagcc gtcatggaat cctttctcta ttgtctcgta
     3601 ttgggattgc tcaaagtcta tgacacttgc cagaaaggat gctatgtcca tactctgggc
     3661 gaataccgaa ttcgttttgt gaataaatcc cagatctgtt cgcactatag aattatcagg
     3721 atttaccaga tagttctgcg atttattatc tgtaaaaacg tagccggctt tgtgtaactt
     3781 tcccgttaag gagcctgctt gaaatgccaa agagcatata atattttttt tgtgctcctc
     3841 gtctgcaaac gctttataca ggttaccccc tccaatatat tcttcgacga tcgtgtcggc
     3901 tgttatcgaa atgagaaagg gagtaggaat tcctagtctt ttcaaattat tcctcatatc
     3961 attcccttca tttcccagtg ccagctttcc tgcaggtggc ggagcggaag gatgtgccat
     4021 cagaacggac attatgctat aagtggccgc caaaaggtat tcgtgaaaat cttttgttaa
     4081 tttatttttc tttataacta ccatgcgccc gtccaactgt tgaactgata catctctctg
     4141 ccagtttacg aaatacaaga attataacac tatatgtgaa tgcttgaatg taaaagtttc
     4201 aatacttaaa ataccgaact atcatatcct atcgaaggga atgatggtgt cacaggtaaa
     4261 tgacggacac agaagggctt caagattctt cacatctatg aatgcttcca gttatgatac
     4321 cgtcgtacgt atcgccactt ttggtcagga ctacctatgg aaaaggcaga ttctggatgt
     4381 tatagataaa caccatctat cttctccttg ttcttcccct cattgttctg atgatcttcc
     4441 agctcatatg catctggacc ttgcatctgg gacaggaata ctatcttcgc tgatagagcg
     4501 ccgtgggaat catggaggac aaagaccaag aaatgtggtc ggtcttgatt tgacattcga
     4561 ttacctgcga accgcaaaaa aaaagtgcag caccggactg ctaacaaacg ggacagcaga
     4621 ggtgttgccg tacagagagc aatcattcga ttcggtcaca tcatcttatc tagcaaaata
     4681 tgtcgatatt gaaagagtag tgagtgagtg ttggcgtgtc cttaaacata gaggtgtcgt
     4741 ggttttccac gactttactt atccgtccaa ctcattggcc agaagccttt ggaatggcta
     4801 ttttaagata ttgcgatcgg ctgccaaaac agtaacttcg tgggcgccag tttttcaaga
     4861 gttggatcag gttgtaaaaa tgagtaactg gcttggacaa accatgcagg cgctagaagg
     4921 tactgggttt aagaacttat ccttcaggtt ctacaccatg agaacgtctg ccattgtttc
     4981 ggccaacaag ccatgaaaga agactctcgt ttgaacgaat ggttcgtacc taagtttggc
     5041 tcattaaaat tccgggcatt tgttggttta ttatttttac cttataccgg aatgtgcata
     5101 tcttttacta tcataggtgc gatgctatca tctacaattc aatgggatag ggttgctgcc
     5161 atagccctaa tttacgggtt agccttaggc gtgtcggctc atgctgccga caatttagga
     5221 tcaaaaaaaa ataagccgtg gggaaggtac tttagcgcaa tgcagttatg gctgttaata
     5281 attttcagtt tagccatagc ctattcgatc ggaatttatt atataatatt tcatgtcccc
     5341 ttgcttgtag tcgtcgctgc cctagaaggt tttttcgtct ttgcatataa ctttgaatta
     5401 tttaaggggg tcttgcataa tgatcttgga tttgtctttt cgtggggggg tcttcctgtg
     5461 ttagggggat acataatgca gacgaatggt gttggaacgt tgccaatcat aatgtctgtg
     5521 tgcgcggctt tagtcagcta catgcatatt agaatatcca ggccatacaa agaactaagg
     5581 aggaagaggt taaacgagcg gcgagccgaa agattggaag tgtatctcaa actaattagt
     5641 ttaggaacta tagcagctgc ttttgctgcc atcttcctta gggtcatctt ttaggtcgtt
     5701 ttttaatact acaactactt tctaattatt gtactattag tagagaggtc gcggatggtc
     5761 atagtatctg ttgtggtaag ggattttgga agtcatatgt tacaaaagat ttctgaactc
     5821 gaaaacaagt cgaatatcaa attacctgtt acgaaaaaaa ttcttttggc tgagacaggt
     5881 acagtcgaac agatcttgag tatattgacg aattcagaaa ccatagtgaa cgttctaaaa
     5941 caaacagagg atcgagaact catactgcga gatgtttgca tagccagcaa gaagaccggc
     6001 gaaacgctag tcaatgcaag gtcgcgcttt tttctagaca atatacctgg agatatcata
     6061 aatcagatta aaagaaagaa cctaggcatc ggaaatataa ttataggtca tgaattagaa
     6121 acattcagaa aaataattaa aataggttac aattcgaaat ccaaatcgat ttttagagtg
     6181 tatcatatta tccaccacgg aaaagtcgcc attgaaatnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagatt gaagccgaaa atagttctca
     6481 gcactattag tgtctttttc gctactagtg aaacctataa tgaaagagag gtggaccggg
     6541 tgggattcga acccacgacc tcggcaatgc caatgccgca tcctaccgga ctagacgacc
     6601 ggccctccaa cttcaacaat cctattgttt ggtattaata cgtttaacga attgcagacg
     6661 ttagatggta aataggatat tattatttat aacaaagctg caacatagtt taattgtaca
     6721 gatgctatga attctatcca gcctgtggca atctacgatt ttgtttatgt ctcctcataa
     6781 tgacgacggc caacgccacc accagaatgg cgatatccag agcaggacca atatcatata
     6841 attaagggcg tttctcttga ttattggtat acgcagtgac agttgagcgg tctatcggag
     6901 cagtgataaa attccaatca ccgagcgatg aggatgatac ttatcgtgcc gagtcagcgg
     6961 agtttctttt actgagaaat cgcagaggtt tctggggctt tccgcaagga cataaagaga
     7021 aaggagagac cgaaatagag actttaaaaa gagaggtagc agaggagact ggtatcgagg
     7081 atatagatat acaatcctat attggaagaa ttcgttattc ctattttaga gcagatggca
     7141 tgaaatccaa caaagaggtg accttttatt ttgcgagcag ccccagtagg ctgataaaga
     7201 tatcaataga acacgagggc tttaaatggg tgacatactc agacgcctta tctctcctca
     7261 accatagaca attaatatca atattgatca gagggcacag aaaaggtctc tattaacttt
     7321 ctgtccatcc cggtatcgca actatccgcc attagacata ctggcaccac taggactacc
     7381 actaatacga actcgtatat ctacggaaat agatttatac ctaagattgg gcgaaatacg
     7441 gaggattaat aacaagatgg ttgccgggaa gcgcatagtt ttgacagcag acagaagttt
     7501 aatgacaaat tatagaggga attttcttta cggtttcatt gcttgcggac cttatgaatt
     7561 agttccagaa tttgtttttg acaagttatt ttgtccaagc gttgaaacag ataaaaatac
     7621 tggtgagata aaggttgcac aatgcggttt gcgaagggtc gaaggcgctc tcttaaaaga
     7681 atacagccgc gaagacattt tccttgcaca cccagaaatg ctggaaaaat gcattgggcc
     7741 tgagactaaa gttgtcggaa ttaacgttat ggatccactt ggaatggctc ccgtcactac
     7801 tacaatgtca cccgaaaatc tgtcttacgt tgccatgaaa ttcaaaaaga tgtgtgcttc
     7861 gattattcaa ctaaaaaaga aatacaactt taaagtggca gtaggcggaa acggtgcatg
     7921 ggagcttgcg aagcctgaca gaatgaaaat tcacggtatc gatacagtag ttataggcga
     7981 agcagacgag cttgcacttg acctattcca tgatctcgag accggcgatg caccagaact
     8041 attgcacaca tttgtcagaa atatcgaaaa cattccaatg atacgagggc cgactataaa
     8101 ctcattgata gaagctatgc gtggctgcgg aagaggctgt gatttctgcg acgttaacaa
     8161 gcgttctaaa aaagatatcc cacttgaaag attacaggca gaagccaaaa taaatctcaa
     8221 atacggtttt gactctattt ggcttcagtc agatgagatg ctactttatg gatgtgacaa
     8281 tcgagatttt atccccaatg ccgatgtaat tttaggtcta tggaaagggt tgaaagaagt
     8341 cggtgcaaat tttattggga ccactcatat gactttatct gcagtggcgt cctcaccgga
     8401 tcttataaag aaactgtctc aggttaataa tatggacaac aacggaagat ggcttgcgac
     8461 gaacctagga attgaaactg tagcaccgag aatggtcaaa aaacatctcg gagtcaagac
     8521 aaagccgttt aggccagaag aatggggagc agtagtgagg gaaggagcaa gaatcctaaa
     8581 ccagaaccac tggtttccag cattgaccct catcataggt tggccagatg agacaaccga
     8641 tgagacacag tacacaattg atttgataga agactttcgt cagatgaata tgagaggatt
     8701 ggtagcacct ttactctatc aagacttttc agaaaagaat tctatgcact ttggcaatct
     8761 caacgaggcg cagttcacgc tcttctggaa atgctggcag cacaacttat gggttataaa
     8821 cgacatcatt ccgatcatta ttagaaataa aacatacggt ccagcaatga aagtgttcat
     8881 ggcgctgttg atcaaagccg ggacttgggg cataatgaga tatcttagag ggttatccaa
     8941 aactctattc aatggtcaga ctccagaaga catcgttgat atctatgcca gaaagaggtc
     9001 tgtaactact tcgcctatgc cgcggcttta gcatgtcgtg tgtgttttgc acggcttctt
     9061 gtcggtgcca gcaaggcttt agttgctgga ttgctggatg gctacaggtg ttatgcatga
     9121 cgacttaatg ccaaactttc ccataaacaa tggctgtact gaatcaggaa cattctaaca
     9181 agagtgccta tcttgacccg tcgcttgtgt tgagtggctg gtcacttttt cttatttgat
     9241 tgctatgctg tttggctaga gctgaaagca tgaagctgtt gacagaagat acatgtagac
     9301 gcagagatat ggcccttttt cactacatgg tgatttttaa ccactgtgaa tgctacatga
     9361 ccataaagga gacatgtaac aaaaagaatg gcagtaccaa agagtccaat aaggcaaaaa
     9421 atagatcaat accccaaagc taaagcattt atagacagca ccgctcgtat tggatcattt
     9481 taacaagtag agcgccttct cccataaaca gtttttggcc ccggtgctgg ctagctaagc
     9541 aagttcacag ttcttgttat tagttggtga ggctcttaat ttcaatctgc acgccattat
     9601 gctacaagaa gacttcgcac cttctccaat atttcttacc agttccataa tgggatccac
     9661 caatagtaaa cctagaatca aatctttcac ataggttacg acgagccaca ggtatcctag
     9721 cttaggatat tggagttaaa actacagggg ctgctaacag ggagagggag aggtgtggtc
     9781 ttcgcaagca cctctcacta ccagttttta ttcttcaatg atgtatacct gggcaaatca
     9841 ttagattaaa aataaaatat gtcctgattt aataccaaac tatgaagcaa gtactatgcg
     9901 aacaggtcgg atttagctgt gactacgtta tagatggaga gacagacgag gatgtcatga
     9961 gaaaggctat agagcatata tgggaatacc atgccataaa accagaagaa atgacttcag
    10021 acatgaaagc gaaaattaag gaaaagatcc aaactctcta aatttgaaag tggctattgc
    10081 aagccgtggc gctactcttc atggcatagt gctcgttaca ttatctcgag cattaataag
    10141 actaggtcaa tcagaaccat aaggtttgaa taagtgtctt agtaaactgt acctttggtt
    10201 ctagcataat ttcgtctctt gtaccgtcct cgactacata tccattatga agaatcatta
    10261 ttttatcggc catactgaag gcctcgcttg catagtgagt cacatagata gttggaatgt
    10321 tgagtctatg aacgatagcc ttaatttcct gtattaaagc gagcttaatt gtgatatcta
    10381 cacttgtaaa tggctcatcc ataaggagaa ggcgaggctc tgttagaaga gtcctggcta
    10441 atgcaacttt ctgctgttct ccgccactaa ggctttgaat atttcttgtt agtagtggtt
    10501 cgatgcctag cagtaatatc atttcttttg tctttataat aaattccctg tcctttggac
    10561 ttcgtaaacc aaatgtaata ttggaaaaaa catccaaatg tggaaataga tatagtctct
    10621 ggaatacata tcctattttt ctcttctcta aaggtatgcc atttaagagc ttttgatcaa
    10681 gaaaaatttt cccttggtca ggttgcaata tgccagctat tagattaagg atagtagtct
    10741 ttccagatcc atttcttccc aacactacaa gtatttgtgc ctcgttaact tccagactta
    10801 atggacctag ttgaaaatta cctagtctct tggagagttc ttcaatcctg atcatgatat
    10861 tgctcactag gcgacactaa acttgtcttt cttctatgtc ctctaagatt cataacccaa
    10921 aaccagaatg caattgaagc aaacgagaat actagcacaa ttaggactcc aggcattaca
    10981 agctttagtc cccctaaagc attgtattcc catagctgga gtgaaatagt ctttggatta
    11041 tacgcgacca tgacattagc cccaaattcg ccaattgccc taatccatgc aagactgatt
    11101 cctgctacta tctcctttgc ggctaatgga atagtgacag tcaagaatgt catagctggt
    11161 cctctaccaa gaattcgaga tgcatgttca taatttatat cgactttttc aatgccagca
    11221 gatgcggtta aaactacaaa tggagagatc acatagacct gtgctaacac aatccctatg
    11281 gttgattggg ttagtctaaa acctgcgtgt tgagccatca gtcctaaagg cgaatcctct
    11341 ccgtaaatat taagaagcaa tgctccagag ataagtggag gtatggccaa tggtatggct
    11401 gtagctactc taagcagttg aacagcttgg gaattgcttc ttgcgataag atatgctagt
    11461 ggtaatccaa gcacgataca tatagcagtt gatgctgttg cagtctccaa actgatcatc
    11521 attgcggatg ttgtttgaga gcttaggaaa gatgttaggt ccaaagtgtc aattctcaaa
    11581 attataccga ggatagggag catagtataa attacaagaa ttgcggccag tcccagaatt
    11641 ataatggtga ataacgacaa aatttctaca acatctctcc aacatataat gcagactcgg
    11701 atgcctctgt catttaatag ttaaaactga gatttcttgt ggtattgcag tcttatttcc
    11761 tcctgcagct agtggtaaca gcttaaaacc atcattttca aagatttttt ttccttggtc
    11821 agaaaacaga aatttgacaa attgtattgc accttcagta tttctaacag tattcggaat
    11881 agtaatatca aatacaattg gtttaccaaa gtttaaagat ccgtttaatt gtgtgcaact
    11941 ggcttgcttg taataacctg caaaagcggg atcacctaga tttatctgtg ggggaaggct
    12001 gataaatgga aatcctctct caatagcttc atgtttgtat gctgggattg catcagcttc
    12061 tcctgtctcc aacaatgtaa atagaagttc ttctggacgc acttgatctt ggtttctttc
    12121 tccttttagt atcgagaagc ttaagctaga gttatgatat aatataccag caagcttggt
    12181 tgctatgaca gtatagcaac cttttggatc tagcaatgga tctgttcgta agaatttgat
    12241 tcctggttta gcaagaacct gataccatgg tatggatcct gtctttactt tttcaaaatc
    12301 tgctacaaaa tgactctttg gattatatgc aataaccaac tcatcagatg caaaaccaag
    12361 atcccatttt gctaaagaag gattattgtc tataagctta gttattggct gttcgcctac
    12421 acctatgaaa acatctggaa atctttgtcc atctattatc atattagcat cctgaattga
    12481 gccatgtcct tcacctttat agccatatcc caaatggctg aaagcaggac ctaccttagt
    12541 ctccataacc gatattagtg agccggcata taggacattt acgtctcctc ccgctgatgc
    12601 tattgttgct tgagcattga ctgattggtt ttcctgtcca aaagaagaat aggacaagaa
    12661 tactagtacg aagcttagaa tcaatagtgt ggtagttgca gttgttcgac ctaaaactat
    12721 cagcggattc aagtgaccat ttcctagtat tacctagcgc aataaataat tacttggatt
    12781 gggtgcggcg cacggatagt cgcagacagt cgaatattta tcgatgagaa atatcgttta
    12841 taagtatgca agggagtaac tgattgtttg tctgactttt cttttattct actgttactt
    12901 tt
//
LOCUS       DAOR01000329            3133 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_53098, whole genome shotgun sequence.
ACCESSION   DAOR01000329 DAOR01000000
VERSION     DAOR01000329.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_53098"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          920 a          651 c          641 g          921 t
ORIGIN      
        1 tgtcaatgga ttgataacga acctatctag acattggtga taatgcagta aaaacctttc
       61 tttgagattg ccatctcgaa tctgcagata tgctgatttg aagcgacaat aaatattgtc
      121 ttatgatagg aggcgaagac aagttagaag cacggtatcc agatataata atatcttagt
      181 tatgagcgaa ttgaaatggg gccaaacacc ttagtaagct ttccttaaaa caatccgtac
      241 tctttattat catatgctgt gaatcttaac aaaaaacttt ctattgtttc tgtaaacagt
      301 tttcaagtgg atcctccttt aaatctacta ttgctagaac atttttaatc tatggggacc
      361 acgatcattg taatcgcact atgcaggaca atcggactga aggaggcaga agaaactgga
      421 ttcctgcacc cttccaccaa gccgcgcaaa aaattgctgc cgatgcagta aaacaatttc
      481 taacggatga ggtcagggtg gtctggcttg gtagcgggcc gacgaaattt caaaattgcc
      541 ctacaaagaa aatcttcaat gtatcgcaac atctacgctg agtattgtaa gttaaaaata
      601 gtaggcgaag accttgtccc caatgttgaa gtcgtttttg atggagccga ccagattgac
      661 aaaaaaagca atatgataaa gggcggagga ggcgctctcc taaaagagaa gattttgcac
      721 tcagcagcca agaacgtcat cattacagca gagtcgacga aatatgtaga atccttcaat
      781 cgatctgtac cgatagaggt acatccattt gcccgatatc tagtcgcgaa aaaactagaa
      841 gaattgtgtg gtcgtccgaa actacgtact tctccgagga ttatgataag gcctttcaat
      901 cctctgtttt ttggcgtgcg accatgctga aaaacgcatt caattcggat ataagcgacc
      961 cgcgaaagct cgagtgaaaa gcaaaggaaa aagtccctat taccaacatt ttcaggcaaa
     1021 caaagagaac gtcgttcctc ttcagcgtta gttataatat gcggactttt ggttcgaatc
     1081 gcaatagttt caattccaat gactttatca gcacttgttt ctgttgtcgg gtgcggggtt
     1141 tagggaaaca ggaccatgtc cgatttaatt gcacaattca taaatggcag tacggatggg
     1201 aatacgtgtc gtaaaaaatg aaggcaaata ttggtgtata ttatgcggct gccaccacta
     1261 ccataattac tggactgtta cagctcgttc tttctagcac gataacagcc atagaaaaag
     1321 gtaatcccag tctacaaata tttttcatag ggataggaat tctgcaatta ttctgggctg
     1381 tgggtataat cagaaaatgg gggagccctt tttactcttt ggccacgggt ctcaacgtag
     1441 cgctgatttt tctgtgggtc ctcttacggc tgccgcaaac gctcgtagga gctgctttgc
     1501 ctatagattc gctgagtatt gcgattgaag cattgcaaat tatctttttt gcgttgtgta
     1561 taatagtaat ggccacaaat gaggatcgac tgactacgcc cgaatcggcg cagcgtgcat
     1621 tcaaaacgaa tataatggat agtatcccaa ttgcgggtaa gttaacttct atggctagta
     1681 ggagggtcgt cttaggaatc ggcgccgtaa tcctgatcat cgggattgct ggaataacct
     1741 acacgatgca gcaagtagtg caacaatacg aaataaagaa aacatcaact cccccctata
     1801 catccgaacc accagtacaa accccatctt ctgtatacat tgaaatagga ttcgttataa
     1861 tcttcattgc gggcttaacc atcatgtcct acggggctac tcttacgagt cgattgtcta
     1921 gacagcagat aggtcgataa tatgaccgac agtgtgaatg agagatgaag taagttgcaa
     1981 tttcgatagc ggaagcttca aattcaaata aaccttaact gaagtgtccg cactttcttc
     2041 tactctaaaa taataaccta gcagagaggt tccttcatac ggtatttcca agggcttaaa
     2101 tgttggtgag aagagttttt ctgctccacc aaaacattgt ttactgatta ctcatgtcgt
     2161 ttgaattctt ggatccgtat gtcgcaagtg atagtccgaa ctttttgaca atactctagc
     2221 agactccttc ggttgttcag gttcatgttt gtgtggttcc tccccggagt gacctcacgc
     2281 tgcctattta caagcactta ctttctatac gattaagggg tcatgagaag aaaattattc
     2341 aagcgatgga gctaaaataa gatacacgac cttataaaac tattttgagt atccgttttt
     2401 ctttggagtt atctgttttt ctttggagtt atctaacctt actcagaaaa acatggtgat
     2461 gacaacgaca aactccggta tttcaagaac atttgtccca tttcctcttc tctctattat
     2521 gctgagctga acattattcc ttccaccata atccaaattc cgcaaattcg tcttccaaca
     2581 ctaacatccc cattccatga agccgactac aataggcttg catcgtgccg aacggcagac
     2641 tatgtatagc tatagagcca tccttggctt ctaaagggaa gaagcagata caataattat
     2701 ctatctactt ttcctaggtg attattcatt ctcgcttttt ttgtcactat ccgaaccttc
     2761 actccaagca ttccaaaaaa tgttaagcca gtatccatta atttttgaga attctctggc
     2821 aaattcattg tacgtatgta taccagccag agtgaaatct tgccatagag ccatgctaag
     2881 agtaaacgga taaaggtagt ctccaccaga tggaatatca aagttatcaa tgttaacctc
     2941 tttgctagga ccagtttcag gaacaggcaa ctcctgttgt tgttcattct tactatctaa
     3001 atcggtcgtc tcaagcggtt cggtagcaac attttggtct gtcacagtaa ctacttcttt
     3061 ttcgatttgc caggtgggtg cagtgggtac agattgtttt ccctcctttg ctgctttatc
     3121 ttgagacagg ggt
//
LOCUS       DAOR01000330            2739 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_135121, whole genome shotgun sequence.
ACCESSION   DAOR01000330 DAOR01000000
VERSION     DAOR01000330.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_135121"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2278..2428
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          815 a          513 c          467 g          793 t
ORIGIN      
        1 acagaaaaaa ggggcagtat ccatttctag gatttctttt gaaaagaaca cagacatcat
       61 aataaaggca aataatatgt tgagcaaaca agaagaccag attaaactat acggatgtcc
      121 tagcaggatg tacgtgcatt tatttcttgg tggatataac ggagacttca acaaatatta
      181 ttatggaata tttgacaaat ctttttcaac tctgtcgaat atactcgctg gagttaagtt
      241 cgttgtagat ttgtctgtac taaaacatga tggtggtgga actcaatata cattcttaga
      301 agcaatccac aacgattgtg ctttaatctt gcatagaaaa tggattgaaa atgtaaaccc
      361 aaaatattgt gacttcaaag aaggctataa ctgttttgcg gttgagaatg aaaaggaatt
      421 agcagatttg ataagaaaag acccagatac aataacggtt gtacaaaatg caaagaggtt
      481 gatgaataga cacattagta tcaaatctga gtggtctaaa ctctaaataa ggttactggg
      541 ccacttcatg tacaatcagt tttaggggcg ttcctgactg atctctattc atcatcatat
      601 tatataagga gatcaaaaag atcgagaaag gaaaagacta cgttctgata cctggtcaga
      661 cgcaaacatg atgttagtct ttgtttcggc gtatgaaccc ttgcataaat cctcttacaa
      721 ttcctgatta ttaaaatact ataattccaa attagccaaa ttattccgac atttcatata
      781 taggatatac gcattatttt gtgcctccaa ctacgtcctt ctttgaagat aacacgcgct
      841 cgtatagttc agaatgctgt gttgcaatca gatcccactt tagtttctct ctaaaatgat
      901 tgacttcttc taagcatcct tcataatctt tatcaagagt taccagtccg tcagcgaagg
      961 cagctggttc tctcttgaca gttataccaa gtcccttgga tgaaaattcc ttaaaaaaag
     1021 gcaagtcaga tgcaacgaag ggcagaccgt gggccaaacc atcaaacatc acccctgagc
     1081 ctgaactaag tttgtaggga agtaataccg catcagccgc atagagtaga atagagaatt
     1141 cttcatcact aaggaaaccc ttttgcagag aaatgaaatc gccagtcctc ttttgcaatc
     1201 ttagactaat tttttcgtcg ttcaagccat actcattttt tgaagagttt gctacgatcg
     1261 cccatccact cggaatttgt attttttcta gaatgtccca acccttaact gctgatctaa
     1321 aaccaacagc aagcgcaatt cgaccatctt ctggtagaga aaaaattctt cttgctgcct
     1381 gcttttttgg cactttcggt acagctggtt cagcaccgtg ataaattagg tttcctccac
     1441 caaccattct tgccatataa tgcgagaaaa ctacaccagc ctcactctgg tccaatttca
     1501 acctatctag cgcgtgaagt gagttgtaat tcaatatata cttccaatat tccttaataa
     1561 tacccaattt tcctgtttga ccggctgact ttactgcttc tactcgactg atccattgct
     1621 taaaattgta agtagaatga aacgttgtga ctacggggat cttgcaacat tcataaaata
     1681 aatcaatatt cgtactgatc tttcttggat taagaggatc caatttcaac ccataggatc
     1741 catgttcata ttgtacatgt actatatccg gatgtaattt gtctactatt tttaagagta
     1801 catctgaatt atgggcattt gtcggggaaa gcccaaaaaa ttggccatgg cccttttcat
     1861 tacatgctac gcacacttca taacctagct tcatcagatt cttggtcaaa ttatgcgtat
     1921 atcggcctag acctcctatt atgggaggat actctacgga gaccatcaga acgcgtgttt
     1981 ttttttcctt tgcttcgatc aagttttatc gtttgcctct tgaaagattt ttaggtgtta
     2041 tacacagaac ttgctataac tttgcaatat actcatcgat ctagaggcat acctgaatta
     2101 actggagaaa tagaatacaa acaaaaaggg agtggtacct cttgcattga tcctgttgat
     2161 ggaatggccg aggcaaaaag ctttataacg aatcaaccga acctttgacc aaactgaact
     2221 gatcgattgc atatactggg ttgagaaatt gggtcaaatt tgtctctcat accaccgnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc aaatttgtct ctcataccac cggacgaaga
     2461 cgaaatagta agatgaatga aagagaatta tgatatgata ccgaaatcgc attactaaaa
     2521 gaggcaatat cagaatagtt gtagtatttt gtggtagtgg tatttcagaa tatcaggata
     2581 catacatagc aatagaaacc agcgctattg gcaatatgag taactgattt tccaaggacg
     2641 agttatccca taatttacgg ttaaaggact gtagaattct tttgtttccc gctcttttca
     2701 tcttcatcca catagacaga ttcatcctcg tcttccctt
//
LOCUS       DAOR01000331            4806 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_27632, whole genome shotgun sequence.
ACCESSION   DAOR01000331 DAOR01000000
VERSION     DAOR01000331.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_27632"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3618..3641
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1584 a          831 c          936 g         1431 t
ORIGIN      
        1 gatggagatt taaaaagttt gaatgcagtg caatgcacaa tattccaatc tataatatat
       61 gccaatatat tcataaacaa tagcagggcg atcgtatgag tagtatagaa accatctcag
      121 tgtcactttt tatgactaga gatgtaaaaa cggaaaaaga agaccaaaat gttctaacag
      181 cttctagaaa catgcatgaa aataatattg gaagcgttat tattgtaaag agagatgaca
      241 aaccagtagg tattatcaca gaaagagatg ttgtacacgt attaggaacg ttaaacctag
      301 cattgctatc cgtaccatta cgcgacataa tgagtacacc tctgatcact atatcaataa
      361 atagctcaat taaagatgca attcagacca tgcagcaaaa gaatattcgt cgccttgtaa
      421 tagttgacaa agaaaaaatg gtaggtatta tcacagataa ggacgtattc agagcaataa
      481 tgaataatca acctctaata ccaagtttgc taagtgatga attactgtca gaacacaaga
      541 caatgtatga gcaatttagc gaatattggt ttggcgacat tcttcataag cgttaaagac
      601 ctggagtgat ttcattttga tttctgagcc aagtgatttg cagaccaaga gtttaagtca
      661 gaaaacggta tgatcgtgtt gttgcagcat gaaaaaataa tttttgccaa ataagagagc
      721 atattcagag gaagttattg tctgcaaaca ttaaatccgt cttcagagaa taggtcgcat
      781 tatatctaag ttcaatgtta tgaaaagaga attctttgga tactcaaatt aaaataaact
      841 cttgcttgag ggtaagcagc atttgtcgtt aattaatact gcactgcata catactggtt
      901 attaaggtgt ggttcttctc agtttgatgg agatatatac ataaaataag tatgatggac
      961 acgaagtata atcgaaacca tgcagcaatg cttgttaata ggagcatagt atgtgaatac
     1021 tttaggctca tcaagaataa agatatcaat agattactag acctttttgc agacgatgct
     1081 acgatctacg aacccttcag taaaatacac ggaggacttc aaggaaggag tgctatcaaa
     1141 gcttttctag aagttgcctt aatggctagt gatggattgc gacatgagat acgatttgaa
     1201 aaacaacatg agaaaccaga tagtagcaac aaatacaata ataatagtaa caacgacaat
     1261 caagttagcg cactagtgac atttgagaga ggaggaaggt taaaagccag atttacattt
     1321 gagttaagtt cagaagagaa ttacaattcc caaaagcaga aaaagattca gtctattcat
     1381 attcagttta ttgaatgagt gtctaatcaa tagagaaaaa ggttactaat gaaaatagtt
     1441 tacagtctta atctctgatt gcactgcaat acactgtact cgttaaacta ttaaagttgg
     1501 tatccctgca tactccacta tgaaagcaat gtgtgcaggt ttaccacatg ccttttctta
     1561 aaacattttt ctgatgatgt agtataatgc cctcgaattc gtgcttacag tctttatctc
     1621 caccgaaacc attattattc caaagcaagc aatgtatgca atttggcatc gtggtgtgag
     1681 agttagttat gatgtgctgc aacaaatcat catgtcgatt ctgcttcaca gacttgacag
     1741 gaaacaaata tcatgggatc taatggaaca aattgggatg taacaaaact ttgcaaaaat
     1801 agcaacagca aggctaggtt acgaacatgt tttaagtgtc atgaaattgc cgacattatc
     1861 gtagtttcgc aggttgtaaa agtcgtggtc cctgttcaat tattattatc tgacacaaaa
     1921 cctcagggag gagagaaacc gcaacagcaa tataataatc aatccgtctc cgccctagat
     1981 atggtgaagg aggattgtca tgctgttttc aatgattcag aattcacgga aattgtagca
     2041 gagctccagg aggttttgaa ccgataaatg aaatatcgaa aattaggaaa aagcggaatt
     2101 aaagtaagtg aaattggctt tggagcatgg actattgctt tagattggtg gacggcgggc
     2161 aacaagaaga ttgacgatga cgaagcaatt cgaatgttaa agcgtgctta tgatcttggt
     2221 atcaactttt atgaaatggc tgatatgtat ggaaatggta aaagcgaaaa actcgtcgct
     2281 caagctttta aagatatgca tggagggcaa ggcgtagatg gagaggaaat cgtatattct
     2341 acaaaatggg gctatgacat gtataccaat gaacaggtcg gtcatagcga attaccacaa
     2401 aaacataacg accctgaatt tctagaatat gctcttcagc aaagcttaaa tcgattgcaa
     2461 acagattgca tcgacgtata cagccttcat aatcctaaga tgaatgctat aaaaaataac
     2521 gaactgttcg gatcgctaga tgctttggta aagagcggca agattagaag tcatggtgtt
     2581 gcgttaggtc cagctatcgg ttggaaagac gaagggatgt tagcgatgga aacccgaaat
     2641 atcacatgcc tgcagacggt ttacaatatc ttagaacagg accctggtag aacctttttt
     2701 gatgtcgcta gaaaaaatga ggtaggagtt atggtaagag ttcctgatgc atccggtgtg
     2761 ctaacaggca aagtcaatga gaagacggta ttcgacaaaa atgatcatcg gagcagcaga
     2821 aaaagggaat ggattattga agcaatgcaa aaggttgaaa agatgaaacc gcttgcacac
     2881 gcaaacgggt ggaatataac agagttagct attaaattca tactgtctca aaaagaagta
     2941 gccgtcgtgt tgcctacagt aattgacatt gaggaactcg agacatttgc gggaatgtcg
     3001 gacggcaaat accttgctga taaggatatc gcagaaataa gaggcctcta cgaaaataac
     3061 ttcgatatca agcctttcgc gtgagtctac ttctatttct cctacttctt cgtagtcaaa
     3121 tgagaagatt gaaaacgtac tgccaaaaga gtggttagct cattcgtgtc aagatggtag
     3181 agtcgacagt tgtttatcat acttgctgac tactactata gtgagaatag tagccttctg
     3241 ttatgaccaa atcttaacat gattttttta tcagtgctgc tatcccgtcc tcaaggctct
     3301 tcgtctttgc ctcgtatcca agttccgact tagcaagcga tatatcagcc ttcgttttct
     3361 gaacgtagtt tttgatcggg ttatcgacat aagtggtata ctttaatttg atccgtagtt
     3421 tcacgacggc tgcgaggaaa tataaagact taactataaa gttgctctat atattattag
     3481 taacagccaa tgtctgcatc agctccctcg agttccaaaa aactaaaaca gcatcgatgt
     3541 aatatttgca acataatttt tgatagtatt gaaacactaa cggctcataa ccgaatggag
     3601 catagcgaag ctagacannn nnnnnnnnnn nnnnnnnnnn nagttgctcc atatattatt
     3661 agtaacaggc aagtgtgtgt cttcttctcc ttcgactgcc aaaaagccta aaacatgatc
     3721 catgtaacat ttgtaatata attctataag ctcatgatag gatggagcat agcgaagcta
     3781 gacattcacc tgcgggtgta tgttagcgaa atcatacttt cattattttt ctaaaggaat
     3841 taagtatgac tgaaggaact gaattaatag tccaagaact caaatcatgt tgttttgtat
     3901 atagcagaag acaatgcaat gataagagta gtaacaggac gagtgctaca aagttaaaga
     3961 taatctctag gatgatgaag cttttactgt attagcagca ttggcagtta aagttacacc
     4021 tccagatgta gttgtagaag taggggatgg aacatttttt atataaaata agttattggt
     4081 cgtatctagt ctgtagctta attccatatt gatactatag ataccgtttc aatgtagata
     4141 ttatcgttat tctacagatt catgaactgg ttgtaaagca tgacttttaa tccagcttct
     4201 actttctgtg cagagaactt cttcgaaagc aggtcttcac ctagtactct tttaattgaa
     4261 gaaaatacta tttcagctat ccatcttcta cctgtatcct ttagctgttt ccatccttca
     4321 tatccaagtt tcttaacaag gaaaacctcg tctcttctta atggacatcc tttggacctg
     4381 gtagatgcat tctttctaat actaatagct ggttcagcat tcatatcatc cagtatattg
     4441 aagttctttc tgttgtcata tgctttgtct ccatacacct tatctacatc atgtctctta
     4501 gctgattcct ttaccagagg accaaacttt tttgtatcat gtacatttcc tttcgttatt
     4561 ctgaatgata ccaccttttt ggacttttca tctacagcaa tgtgcaattt gacaaattcc
     4621 ttcttttcac gtatccattt ttcctcgatg tagtcacctt tcttagatat agttaaacct
     4681 gatgcatcga ctatcaatgt tattggttta tcatcttctt ctccttcaaa acctatctcc
     4741 aaaattgatg gtttaatcct aatcattctt ctcctaatat gggtgaagtg tatttcctca
     4801 acaatc
//
LOCUS       DAOR01000332            5031 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_54867, whole genome shotgun sequence.
ACCESSION   DAOR01000332 DAOR01000000
VERSION     DAOR01000332.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_54867"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1552 a         1003 c          913 g         1563 t
ORIGIN      
        1 gataagtgcc gcaataatga tattgtaagc atgctccgaa agtggcatat caaacaattt
       61 gttgtttata atcgatttat ctactattaa atggggtgtt atgttctgtg agatttttga
      121 cattagctag ccagaattat catttcttcg aatttcaagt atacctttac cagttagtct
      181 tatccttccg gaacttcgtg acgaataaga gacagtcaca gaagatacct cgtctcaaat
      241 gaagtatcat taacagaatc tgccataaat ctcactttaa tttctaaacc taatttgtat
      301 tgcttctttg aatgagagca gtaccgcgct agcagaaaat ttgagaaata aaacaattgc
      361 tgcttactct gcttcagttc agtagaattt tgcagccctg tggtgggatt tagtaaaagc
      421 ttctctgcgg gatgttaaat aacgcagcat aaatcaatgc gaaaatttca atatcgctat
      481 tacatgaaat gtgaaaggtt gtcgcctgga gtcaaattgc caaatgaaag ttcataaaac
      541 gcttgatcct gtattgagat aatgtactct aagtacttta tattggaaaa acttttgttc
      601 ctagatgaca aattgaaaat acatcaatgc gccatttcgg ttgagtgcta accccaatag
      661 ctgatatcat tgctgctcat tggccaactt aagatgacgt tatggggcca acattcaacc
      721 cattacaatt tatttttata caatcatgat tgctggttct cctgttctta catcaaattt
      781 ggagccatgc cagtgacatt caacttcgta tccctcaagc gtgccatcag ctagaggact
      841 accttcatgc ggaaacactg ccaaatgttc tgcacgctcg tgactaaact gccaacagaa
      901 gacgtggaat agcagctcaa tagttgactg tacaataaag aaactattat caggaggaaa
      961 gaaaagaagt cgaatccttt tggtatgtac ttcttttctt ttagaaacta aaatgacgaa
     1021 tcatacggca caagaagttg cagaggaatt atctagtttg gctagcggat gcctttattc
     1081 attttccctt gtggcttgcg gtccaacttg gggaagagtt atgccgattt tcatcctact
     1141 actagattat ggaaatgaac cttataaact cgaaggttta cgaattatac aaactcctct
     1201 actaacgtta gagaattagc gaaatgattc agccttcaaa ggttacttcg tctcgtgtca
     1261 ttttgcgaaa gttatcataa aactcccaca tcataactga gctttgcgaa tttcatcatc
     1321 tgatctagta tacgatgact atgttgaaaa gatgaggaag aaaggcaaat gcctgaaacc
     1381 tataaagttc tagattatat aaaatacaag gagtattata gtattctgct tcatacagct
     1441 tgcgtgttag ttagtctaac gcatcatcaa aagtttgaaa ttgtagggcg aatgtttttc
     1501 tgtgagaacc cctccacttc tcttcaatca tcattgccga tctcttttcc tcgggcatcg
     1561 tgttttttag tagatgattc tatttctaac ttcttcaatg tcgattgtga ttgtgtcatc
     1621 tttcttttta aaattaatac aagtgcaata gatatagcag aaatcaatgc agctgttaga
     1681 aatatcaggt tataggatat tggtgaagga aaagaaccaa cactattacc actaactcct
     1741 tttacaagcg cctggtttgt ttgcatgtat attcctgcaa ttgcaggtcc aattgagctt
     1801 ccaattaaca ccagtacgac tgacatccca agtgatactc cactaaatcg aagtggtgta
     1861 tactccatcg tgatattaaa tgcacccact tgtatcaatg agattccagc agctactata
     1921 gccagattta ttgcaactat agattctgtt gagtgaaaca taaacaggct gaaaaagcca
     1981 acggtcataa tgatagttcc tgctacagtt ggtttaagat tacctatctt agacacaata
     2041 aaaccagaag aaggagcaaa agctaagaac gcgatcataa aaggtaattg aaccattgca
     2101 ctcgcaacag catctccacc aaatccaagc ggctgagggc ttcttactaa tatcggcgta
     2161 gtctgatata ccataaacat agtcattcca aaaatcaaga gaaggatatt tgcaggaaga
     2221 agtattttat gtgtcattaa acctagctca attaatggag atgccgctct tctctctata
     2281 ataacaaata agactaatga tcccgtagac aataaggcta atgagactac tacaatctct
     2341 gtagaatatg cgttgacatt attactacta ctaccattcc caatatatgt aagagttagt
     2401 aaaaatgagg ctatagtaat agccaaagtt atggcaccct ttacatcgag gctttgcctt
     2461 tcactaatat tagcaccaac ttccgttggg ttcggagaat acactctact ggacttgcta
     2521 tcagcatcag cttgtgctaa atcaccttct ttttctacta cttcagcctt cttattatca
     2581 ccgtgaatga atctattgat tattacccaa agaatgatag caactggtat tatcgaaaga
     2641 aatgtagcat gccagccaaa gctttggatt atacttccac ctattgctaa tcccaccact
     2701 gatcctgcag caaacattga gctgaatact cctacgccta ttgcaagctt ctcctttggc
     2761 aattgatccc ttataattcc gaaagctatt ggaaacatgg atacgccaat tccttgaata
     2821 actctggcag taagaagaaa ggagatgtta gtcgagactc ctccagcaga aattcctatg
     2881 atgtaaatta tcagtattat tacgaccatt ttctttcgtc cgtagatatc ggataacttt
     2941 ccagcaatgg gagtcgcaac cgcagctgag atgagatatg ctgtaagtat ccaagacgat
     3001 gtactatatg agatatgaaa gtcactaatt atatcacgga tagctggtaa taacattgtc
     3061 tcaccataca ttgtaattag gatcgtcgat cctagaatcg caagtgtaat ccatgcagag
     3121 gttctaattt tttcagacgt ttctggcaac gctggttttg atgatgatgg cggttgatgt
     3181 agatgttttt tatgggcatc cctatcgtgg atacgttcgt ctttgtttaa atcatccatc
     3241 acaatcactg gctttcgatg tcaatacctt tttccaatag tgttaccttc aattatataa
     3301 cccttgatct ttttggttct gttaacatag cgtcggattt gtagagtgtt aacccgcgtc
     3361 ggatcgcggc aatttatctg caatagccgt agctctgctc acgatttcct caatcttgtt
     3421 cttctctggc agcggaatat tgttcttctt ggaataatga agcaaccagc gaatcgaaga
     3481 ggacaagtat tccaatagct cttcatttat tcttttaaat tgcatcgcat acttcaattc
     3541 ttgatatgcg gctgtcatga gatgatcagc attatccgta aatattctca ccccttttat
     3601 cgcggtttgt tctgtcttcg agtgcgtcct taatgatatc aaggccttcc ttcacctttt
     3661 gatattgtga ttccgcttcg cttttaattt ctttaaattc ttctctatct ggtaattttt
     3721 tcacggtttt tgcaaggctg ataattgcag gagcgctaac ggcagctagc ctaaacaaaa
     3781 ttgatgtttc aacaaaataa gcccgcgaaa atgttgttaa agtgtccttg aggtcatcga
     3841 ctgttttcga cgttcttatt agattgttaa agacaccaat ccccttgtca tgaatctgaa
     3901 tataatcatt tagtatattc ataaccacgg tttcgtcttc tgtcattttt ctttcgcctt
     3961 accctctgcc aaccacatgt caatagcctc aataattgcc tctgtaaaat ttccacgttt
     4021 agcacccatt cgtctaccga tttccattcg taatttattt tctaagctat caggcaaatt
     4081 gatatcaatt cgacccattg tgtattatac acaaatgtgt ttaataagga tatcccgtat
     4141 aaccacaatc cttatatggc tatgtgtagt ctacacatat cagtatgaca tgagcaaaac
     4201 agctataaac cgtcagcaag aaggattagt cattgctaac attaacggcg cgataaaaag
     4261 aattagcgat tccgcataca aagtaaaatc acaaaatgct aatggagaat atgatgtttg
     4321 ctcgactggc ttaggctggg tttgctcatg tcctgaccac aaatttcgtg gcgtcaaatg
     4381 taaacacgtt tttgctgttg aaatctcatt cgcattacgt aaggaagtcg aagtcgcgcg
     4441 cattgagcct atcgatacac aatgttgcat atactgccaa tcttcgtgga tcgtcagaga
     4501 tggtctaaga cataacaagt atggcgcgat tcaaaaattc aattgtagag gatgtaaccg
     4561 atatttcaca atcaatttag gatttgagaa aatgcacgct acgccgcaaa tgataacatc
     4621 tgcattacaa ttgtatttca caggcgaatc gttcagaaat gtgcagaaat tcttgaaatt
     4681 acaaggcgtg aagatgagtc atgtggctat ttacaaatgg ataaaaaagt atgttacttt
     4741 aatgaagtcg tatttagaaa aaataacccc aaatgtttcg gacgcttggc gcgccgacga
     4801 attgtatttg aaagtcaaag gaaatccaaa atatctattt gcgcttatgg acgaccaaac
     4861 aagattctgg atcgcacagc aagtagctga tacaaaatat acttcgaata ttcagccatt
     4921 gtttaaagac gcaaaacaaa ttgcgggtaa aaggccaaat accgtgatta cagatggcgc
     4981 gcaaaatttt gctgacgcaa tcaaaaaaga atttttcaca atcaacaatc c
//
LOCUS       DAOR01000333            8454 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_56894, whole genome shotgun sequence.
ACCESSION   DAOR01000333 DAOR01000000
VERSION     DAOR01000333.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_56894"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         2843 a         1626 c         1552 g         2433 t
ORIGIN      
        1 tcatctatct tttttgaaag atcacctact ttcggatgac tcaaaagacg gcctatttca
       61 tccaattctg aactaggcct ttttactact cctcttttca ttgtgataga acgatacgta
      121 tctattggat taatctcgat cgctaaagat gcaggtcttc ctttagaagc gggtagcatg
      181 aacagaaatg caccagctat attagtagcc tttctttccc aatttctacc atcattgaga
      241 aattgtctca gcctttcatt tgctttttct gtcatgttat gttaccacgt agaggatatt
      301 agtttgtatt tggctttaca tatttcaagc aattctcctt cagaaattaa tataaaccgt
      361 tttaaacccg tatttgtact catgacgatt ggtaatacca ccttaaatac gacaatcaca
      421 gtgtctagat ataacaaatg aaaattgagg agggtaatgc aaacaaacag accagaataa
      481 acaaacagtt ttcaatcagc ataattctcg cattagtgat attcatgatt agtggttcga
      541 cagcgaaaat gagttatgca gcaccccaaa cgttaccgtc ctgcactgac cccacaggcc
      601 aaaacctccc atgcatgatg gtcatatcta cattaccgcc accacccaat tcgctccaat
      661 gtcaggagac ttcaggtcaa atcctaccat gttcctatac tacgcagaat ctgagcaacg
      721 gtgaacaaat cgtggctatc acagtatatg taccagccag ttatgtgttc acaggctacg
      781 gcccgtggac actagtaaag caagtagtac atgagacaaa gacaagaata gtccatgtac
      841 ctgaatgtcc aaaaggccaa acattggatc ctaaaacaca caagtgtgta gacatcccat
      901 attgtccacc acctaaaggc tttcacatcg actgcataac gcacaagtgt gtcccagata
      961 actgtcagcc aggtcaagta tacaatccga ttaccgggaa atgcgaagca caaaaaccgt
     1021 gtgaacgtcc agatgctgat gccaaaacag atcttgacaa aaatctcaaa acgaaggact
     1081 gcgacatagg tgatgcaaat gaacatggtg tgacaccacc gccaccacca tcgacctgca
     1141 aagacaatac gacatgccct tatgtcaata cgacaacatc attagcatca tcagcatcaa
     1201 tacaacctag caacacatca attccatcag caggaggttc gggtacagga tcaatatgtg
     1261 taaataattg cacaggaact ccaccagcag tagtgtgtac aaagaatcca aacgatccat
     1321 catgcacaca aacattgata tcaccaccgc caacgtcgcc gacaacaaaa acatgttctg
     1381 acggatcagt aatagatgct agtgcaactt gtccgtcaac gccatcaact agtaataatc
     1441 caccaccaag caataatcca agtccaccag gcagtagcaa taatggtgga ggaggaagtg
     1501 gtagtggaag cagtggcagc ggaggatcta gtccgtctca accaactaca gttagttgat
     1561 ctaaaatgaa aagaggggaa tccatatttc ttactgttcc tttattcttc ttaataccaa
     1621 tccgcaacaa caacaggaat cgtaataata agtagtttgt tacatcgcga taatatgagt
     1681 taaagatgta cgaaatccgc tttaaacatc tcaacgacat tggtaccata gcattctatc
     1741 aatcttactt ctgggagttg ttcgtttttg tttaccttca actgaaggcc aatagcacga
     1801 ttgacgccat ccactactag tcttttaccg aacccagtgt gatagactca tgttaacttg
     1861 atctccttag cctgtacaag gattctattt aactagattt cacctctgaa ggtgcttgta
     1921 aagaatattc atctaaggca aagtttctgg cagtaagatc ttcccaagtc tttccgtctt
     1981 tgtatttcgg aagaaaggcc ttttctatcg ttgacttcca ttgttcttgg gaacgaatag
     2041 tacattttct tgctcttaca tcacccaaga accgtcgttc ccagataacg cgaatataat
     2101 tgtcctgact agtagcagac gccataaact cgttatttga tatttcattc cattgtacgt
     2161 tttccaatcg attagttagg taaaccaggt aaggatcaga taaatctctt tccttgtttc
     2221 caatccgtcc aacgtgataa tcatgacagc atcaggcgga taaggaagag gaccatcatg
     2281 tagtaacaac tgtactacag gaagacgctg ccataaaaaa aagtttaata ctattggtat
     2341 taccataatg aaagaagcac gttataattg gtaaggcaga gaaatcttag tcagcaagaa
     2401 atcaaaatta gaatcttgtc ttattcatac aataaagatg aaggatcaaa tgcacataat
     2461 atacaatttc acgctgttcc tggtcatact caggaagcaa gttgtttcaa attattgctt
     2521 gaagaactat gtaacctaga gagaatacaa gaagtggata tgagtcatgt aactaaagga
     2581 cgggtaatat acaaaataac agataaaggg agacagacca tcatccattt acgaaatcct
     2641 cttattaaag aatttctagg atcagtcgat gaagaaatct aagatatcca taatacagaa
     2701 tagcctacct tctcttccaa tattaaatca attttgaaat ttgccttcaa ctcttttatc
     2761 atttgtttgt ccaatctcac cttatccatg aaggattcat cgtaggcttg ggcaacacat
     2821 attactcgaa aaatgcgagt aaacttttcc ttcgctattc ttgttagttg tctgagatct
     2881 tctggtgtta caattttatc tttgaagtcc ttaactataa agtaaccttc atccgtttta
     2941 taagcaacat ctaaaacata cgaatctatt gtataattca gactttccga gatatctgca
     3001 tgaatagtat ttcgggttat cttccttttt gaaagtagcg ccttcacgaa tgatggaaaa
     3061 gtaggcttat ctaaaggtga tatgtagaga tcagatctca attcaggaaa tacgagtttg
     3121 gatagattga atactttttc gcctgtcgaa ctaccttttg gtatcgtggt atcaaaaata
     3181 agaatatagg cgctctccaa atagtcttcg tcccattctt gaagttttct gtatgcatga
     3241 ggagccttaa ttacaaacca gccgaagacg taagcagata tcgctattga tataactaaa
     3301 gatacgaaga ccagattttg tgttgctata gacgttgcat tgagccaagc gatcatgctt
     3361 aaaatattaa aaaataatac tcctataacc aatctaagca accagcgact ggcccctctt
     3421 agtgatttga caataacgta ggctctttgt gaaagactta aagtagattg aggttcctcc
     3481 atcaccaatt cgcaaagttt aaacattttt ttgtatataa gcaagtctca caaaatgtga
     3541 gaacagtttc actaatagta tttgcagtat catagaaaat gttgatacaa tcaaaacttc
     3601 tgcggatttc actataagct tttcttcatt atgacttatg acatgaattt gtctgcaaaa
     3661 aaaacaattt gcgtacgccc gcaagtaaat atggcgagtg cgccggtgcg aatgaaagac
     3721 acggaaaaac aatcctcaag acttgaaagc aaaactaaca cagaatcgaa taatggggat
     3781 gactagagaa tggaagatta cataactctt atgaaagaag cacgtgagca aatgaaatca
     3841 aagagcggaa cagtggcggt gaaggacctg actttgtgcc aaagacaaaa aattttcaaa
     3901 attgtcgacc cgattccaat gactgatgaa gtattgtaca attatgtttc gggtcaggcg
     3961 gcccacgatg ttataggaag actcttcatg atgtttccaa acagattcaa gttagaaatg
     4021 cagatacagt atgaaaatgt aaaaggaaca attgacatct atgacaaact tctacatacc
     4081 gtaatagacg ttaagacaac caagtcgaat atcatgttga aaccgaacag gtgggatgag
     4141 caacaattaa ggtattacat gtcaatggtg gattctgagg aaggggcggt tatttatcag
     4201 atgaacaatg cttcaaaata tttgatattt ccaattcgta tgaatgaact ccaacgtaag
     4261 gagcatttgg ggaaacttgt agaagaagcg aggtctttcc aacatgctat tgacttggga
     4321 gatccctctc ttctaaaagg tgtctacgac gacaatgaac gaaaatggtt gtgtaagacg
     4381 tgcccatatt tggaagcgtg taatgccatc agaaagattc atgcaaaccc tacggaggct
     4441 gcttgattat tgtcagtaca ttaaaagaaa tctaatctaa tgagaaggtg tgttgattgt
     4501 gcctctagcc acacatatac taaaagaact aaaaatggca ctccataccc tcactggtat
     4561 cgtaatccct acaaagaagg aacatgggtt tgtggcaagt gtcataaata tcgacagaac
     4621 cacggcaatt atccgaccaa agaagacttg gcggaactac gcaatcaacg actgaatctt
     4681 agtgatggcg ttcataaacg gacctaactt ccaacaatcc attaaccaca tcgacaggac
     4741 tataaaagac gcacaagaag ccagaaattg cctaactcaa atgagaacat atatcaacac
     4801 aaatatcgac aaatcgatca aattccagaa ttctatcgaa gagaatctga accgtgcgaa
     4861 ggatctgata tggaaactaa gggagttgct taacagcaac ccctcagaca cttcagactt
     4921 ctaacaaaaa taaatagaat ttatatacaa agcctgtaga aaatgcctat tgttacttaa
     4981 cgacatatgg aaagaatacg aaaacacgtc gaccaaattg gtcgttattt ggcaagattt
     5041 tgtgataaat aattacaatt tgttggtgag ccttattgct ctgagaacac agggcgctga
     5101 tctttactta catcaatggg tagcggatga gtataagaaa agtaaagaat cctttaacaa
     5161 agcatgactc atgagtctaa ctcccagcgg ggtggtgaat ctgcattccg cggattagac
     5221 taccaaaaaa agttcattgc atatttatca tcagaaatgc tgcgagggac acaaccaata
     5281 aaaggaatta cttgcgaaca tctagacgat atagaagtag aacaaaatga ttcaacatta
     5341 agatattatc aaattaaatc aaccacaagt catacattat ccaaatcaca aataatagaa
     5401 agcgtaaaat tattcttgtc aattcaagca agaaaaggca cagacgcgag ccaggaatac
     5461 atcctactgt cgaatgcaca cataaggaaa atcaccaaag accttttggt gttatatccg
     5521 ttcaaaaatc tggattatga aattaagaaa gagataggat ctctaaaaga ggtgcaaagt
     5581 acaaatcatc tggaaaaaat atttttcatg agaggccctt ctgtcgaaga aataagtagc
     5641 gttataactg caaatattgt cagagcattg aaagacaact atgattttat agcgataaca
     5701 aatgatttgc tgcatcatat taatcgtatg tgcgcaggtc cagtagacct ggaagatgta
     5761 actatgatca atgaaggaga aagagaccaa tatgtgctaa agcataagac cataaatatg
     5821 agcataataa ttgggattat cgaaaataag aaaaaaattc tgagtgaaca tgtaattgct
     5881 agtcaccttc cggccacaac ctcgccatac gactcgccaa taagagatcc gactaaagga
     5941 gaaacaaaaa agatttggga tttagtagaa gaatatagtt gcttcgctga aggagatgag
     6001 ttacagttca catacctgca aaactttaac aaagtttcta tggagtttaa gatgtataag
     6061 aatacgaaat ttttagattt tttaaataat cagataaaaa ccaccaacaa caaacatatc
     6121 atattagaat gtcttattat cttacataat ctattattaa gcagtaaaaa ggaaggtcga
     6181 tactcttttg tggaatacat taagaaagag tattttcccc ttctaaaaca acatctagaa
     6241 tctggaaatg aaacatttga atattcttat tctaaaatag agcagatctt cgaagaactc
     6301 aagattgcca acgaagaaat gtgcgaaatg tattggaaaa gaatggtcac catgattcat
     6361 aagataagta aaacagggat tacagacaat agatggtggc tttgtataaa tgccctcaat
     6421 aaatgcaaaa ttaaaacagt gtggagaaaa tggctaatta ccaaagataa atactatgat
     6481 attaaaaaag cggttatgaa ggaattagca gactcttcac taatatagga caagagtcta
     6541 tatcgaaatg gttttgtctc ggagcaatat taaacgataa taatatgttg ctgcatatca
     6601 ttcgaatcga taatgtttag gacacagcct caacgcatct aagagatcga gttgtcctgc
     6661 tgctgttttc tttaccctcc ctttagcaga agtcattgct atgcgcaaac ccaaaatcag
     6721 atcatcaaat tgagggggta ttgcaaccac gttattagaa aatagcagat gcaaatctgt
     6781 tacattcagc atctagcaaa ttgatatatt gctatcaatg atactaaaga tttgaactgt
     6841 ggctctattc gcgagttatg ctacctcgta aaagagcaaa agaatggaca gccgtgaaca
     6901 aactacatta gcatagtatg cttagaaatt ctaatgtcgc cattcttatt gacatcaact
     6961 attccgagat tatttctaat ccatctgaac caagaaacta tagtctgtgc tcgccttcca
     7021 agagttgaac cagtcaggtg tgagtttttc tttaacaagt ctacgatgtg ttgtctgttg
     7081 atgaccttgc caggttctat tgaaatatct aaaaaaactt ggttaactat ggggaactca
     7141 agaagcagtt tgcaaatata ctgggacttt cgttgtgaag aaaggctaag gtaggcttct
     7201 cctctatcgg aaagtttgta tctgccgttc tgatctcttg aaaccagtcc tagaatttct
     7261 gacgcatgtc tatagtaaga actctggcgg ttaacgaaac caaatgcatg ttttattttg
     7321 tctgcagtat catatccttc gaaaattcta agtggaaatt cactaatttt gttcacatca
     7381 ttggcttgcg ggatttcttt tttattctct attggtctaa cattttgata ttccttaact
     7441 gacgctcttt ttgatatctt tatttggtac tgtttgcatt gaactagttt aatggattcg
     7501 aaactatcat acggatcgaa ttcgtactcc caaaataaat acaaccttct ctctacggct
     7561 ttcttaagac agaaaaagaa attccttact atttttccac tttgatatgc cgttctaaac
     7621 ggatagtaga gttggcgtat tgcaaaggag gtaggaacac caatttttgc ttcaaataat
     7681 ataatctgtc caggattctc aaatcctgca tctacctcta tttgtgcacg attaacggtt
     7741 agttgtgacc tgtccacctc aaaactgaaa tttggtgttg ttgtcctacc tctaaatgtt
     7801 tgaataagat tttgtgttcc gatcaccttt tctagcaatc cgcatgaagt tgcatactcg
     7861 aggagaactc cttcgctttc ggctttcaaa aaagacgcag aaatgggtaa ttgagtagta
     7921 tgtgtaattg gtttcgtatc agttatcggt tctaacttgt gatatccttc tcctttaaca
     7981 atagcatatt ctttcctact aactggtaac agaaagagtt tattttctct gaagactctt
     8041 ggcagatttt ctattctgtc tatcttggcc atcaacctcg catcttcatt tgtgatctgt
     8101 ttgatctgct tagcagttat gttattgatg ggttttgaga catcgatgtg tagttgggtg
     8161 acaatatcag accacttttc aattttgcga gaaatttgtg gtgattggtt cgttcgattg
     8221 ttaccatccg cagcagccaa taagcaccat tccatttatg aagagtagtt cgaaataagc
     8281 aattccttat ggcctgaacg tcttgatgcc ttgctattga taacgcgtga tacgtttact
     8341 tctttaataa agccagaaaa gtcagaatat aattttctaa tgaatggtgt atctgaattg
     8401 cttaagagta ctttgcactt tctatcattt aattcaacaa agagtttggc aagt
//
LOCUS       DAOR01000334            7812 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_12866, whole genome shotgun sequence.
ACCESSION   DAOR01000334 DAOR01000000
VERSION     DAOR01000334.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_12866"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    2944..3193
                     /estimated_length=250
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2233 a         1463 c         1430 g         2436 t
ORIGIN      
        1 acttagctat caaagtggat ggtccgaaag aatcagatat ccttctgcta ctggggtcaa
       61 agacattgga caaaattcct gttggatggc gagacggagc tttaggtgag aaagggataa
      121 tacaccttga tggtgacctg catattcctc agaacgagac tatgaaatgg tcggtgatat
      181 atgcatcgga ttgttatcaa ggttaaacca ttatggcatc aaatactcct atcgttttcg
      241 atggcaaacg ctatagaata cgtggcgcag atttgacaaa ttcgttatta tatttttaga
      301 tcatgagttc ctgcattcat cttcttcata cttattaccg tgccttgatt gatcggctac
      361 ctctttattc cctttaagca aggtcttatc acatacaata atcatgtggt tgggaatgct
      421 aaaacagttg ccgtaacttt tgtagatgga aatacctata cggcaaaggt ggtaggtacc
      481 cattcctata ttgcaatata tgctgagatt atctagctga ggcctatttc tttgtgtttg
      541 tatttgtctc tgaccgagca gcttcaagtc cagatgtcgg aatcaccagc gctagtgctg
      601 ctgcgaagtt cacctgtctt ttctagctcg agatttgtag tctccaatac cttagtaata
      661 ctatcgttac gatcgagtct tccgagtacc gtctcgatag cctttcttag tgaaaatttt
      721 aattcatttg gagcaaccac ctctccctta gcagcttgtg tgagaggaga gaattcgctg
      781 attcgcgtta gctgttcgat ctgtgtggtt aaaatagaac tcatgttctg catcatcgtt
      841 acattatttt gactttgtgt agctaagcta gaactcatgt tctgcatcat cgatgcatta
      901 ttttgaagct cagatttaga cttctgtatt tttggttcaa atccgagctt ggcgtcatac
      961 tcttcctcca catccttcag aaacttttga agagcttcct ttggattcat ctggtttgca
     1021 actccgattt ctctaacctt atgccaaagg agcttctgct ctttgagtcc aatgcccatg
     1081 tcctccagct ccccatatat cgcgagagtt tgactatgaa cctctatctg cgactcgaaa
     1141 tatttacaat cgctttcaag cttgtctttt ctctttgttg aaaagtctac actatccttg
     1201 agctcttttt ctacgatttg gagctgttcg aaattagaaa ctttagaaac aagagtattc
     1261 acatcatagc ctagctcacg tacgtcatta atggtcctta tggtcagtgg agcatcgacc
     1321 atggctactt ggtgtttgtc catttctctt ctgaaattcg agaattcatt gagatctgtg
     1381 atggttactg cttcgtcttc catggcacta gctagccttc tttttgcctc tgatgccacc
     1441 gattcaagtg tttgtatttc ttcttctaag ttctttttcc tggctatctg ttcatcgata
     1501 tgagcaggta tttcatccca gggtacagac tctgagagat cggtcagcaa ttttagatcg
     1561 tgtgaaagcc tttcaggttg taaacccaat tttgcgcacc ggtcatacgt ttctgacatg
     1621 aatgaatgga aagcgtcctc acctaatcct aatttgctca tcatataagc aactctcgct
     1681 cctactgcac atctaggagc tgtgatccgt aattttcgta gcgccagagc gaattcacgt
     1741 aactcgtctg cgacggcctc ttctaagccg gcactccact ctcgaataat gttggttatg
     1801 gtgccggtac ttagattgtg cttgtccgct atttggtctc tggtaagacc cctaagccaa
     1861 tcttcaataa tcaaggcttt aagagctcca gtaactcgcg gttgcatcgc aaattaattg
     1921 gcggtcgtcc atattaatat taaccatcgt caaaatatga gaactcatca tcatcatcgt
     1981 cacgacgcac gatcataccc acccacttcc gggccagtac tggacgacac tttcgttctt
     2041 gtccttataa caattcttcc ataactaagc tcacagattt ttccctagta ggatgttaca
     2101 taaagtctac cataacttta ttccaaaatt agtcaataat tagaataaaa aagccccgct
     2161 catggcaggc agcaaccacg gttaaattat aactataatt attggattat tatcaatgaa
     2221 aaccaagaat aaacaacccg aaaaaagcat tgtcatactg ctgtccgctg ttgtgtccat
     2281 ggttgtttac tcttgttacc tttaccttta acaaatgaag ccatagagaa agtgtttgca
     2341 gcttcgacca tgttaccgtc tactacacaa gctactaccc ccataaattc aatagatgcg
     2401 gaaaccaaaa ataatagcct cgtcgtggca caaactccat tttatcaagc agagtttggg
     2461 aagctgatag gtcaaacggt actgaatact ggaggagttc ctcagataca agagtctatc
     2521 atagaaaatg gaactatgaa tggtgttggt aatgttacaa acctcgaaac atgggttgat
     2581 actttcaaat ctcctgcaat agcttatggt gttggccaag ggatactaac tacgaaagat
     2641 aaacaaaggg caacttggat tgcacatgac atcggcagga ccaataataa tggggcaata
     2701 acatatcatg gggtgatgtt ttttagtagt aactctacgg gtaaacttgc ttttcttaac
     2761 aacttggaag gactctacat tacagaggta aatggtagca agcaaacaac aaaaatgtgg
     2821 gagtggaaat aaaagtcatt aggatatttt taactgccca atagaaatag caaagagtac
     2881 aacaaaccaa aaaatccctt gtttcttttt tattgttttt actttttaaa ttgagagtag
     2941 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnncattctt tgcaatctct tcattccaag tttcttctgt atatccgtgg
     3241 acttcgattc catgcttctt tacgttttgt aataattctt cttcagtttc accatgcatc
     3301 gtgtgtgtac atactcgatc gccagcatct ttgcagttga ggcttaaagt cattaagagc
     3361 ataatgtgat tcacgaagtt aaacatttcg tccatgaaaa ttaattggta tacactaggt
     3421 gattgtagca tccataatga aatatgcgca gcagctaaac atagtttgtt cattttcttt
     3481 tggctaggaa ggttctcagt tcagatatat tgattgcagt ttgtcaactt tcaagcccaa
     3541 tatagacaag tacaactcta cgtttccaaa gatgtgcatt atctgggtca ttatcagtta
     3601 caatcgcgtt ttcggtgatc ttgacggatt gtacaatgga gaacgcgaca gcacaggaga
     3661 accgcgacgc actctcaaca cgttccgaat actgctacta cgcaatttgc cgcactcgtt
     3721 tccgagtctt tgagaatatt tccttgtttt actaccttct tgtgcggagt accattattc
     3781 tcttgttttt gaacatctac tgaacaaacg ctatcatcca ccacgatagc aacagttgtc
     3841 ctattagtca tttcatgaaa gaaccaaata tttaattgta aggttgcatc aatagaactg
     3901 ttcacgtaga agctaccaat cttacaggta gataggcatc ttagtattgt tgaacaattc
     3961 gaccaaagca gaataaataa agggcaagcg gaccagtgta gaatttatcg actacgatag
     4021 gttcaatatc gatcaaatgt ccaaaagtga cggttaagga aaaggttatt attcttttaa
     4081 ttccctgagt tctttgtatc catatccaaa ctctgcatga accgctttta gtaattgcat
     4141 aaactttttg tctttggaga gaattgtttt cattgtgctt tcaagttcgt atattgcaac
     4201 tctagcgcta tcatcatcaa catcatcaga attgaccatc agaagtatat ttgattcact
     4261 atacattact cccagtctaa atgctatgag cttagcatgg aattgtaaat ctgattctaa
     4321 tattttggca tgcctctctc gatctccatt tttcagattc atattggttg aatttgctgt
     4381 agtagatttt ttgatagtat cgattctatg gagagtcgcc ctacaacatt cctgcagata
     4441 ttgagttatt atagagtaaa agcgagcagt agaacgttct attgtatcta cttcgaaata
     4501 tggaaacaga agagtgttta atattatgtt atcttggtat cgtattaaca attgtggtgg
     4561 ataacccaat agattggaaa aaatgtagaa taatccagag gtagttaaca cataaaatat
     4621 ttccctaccc aggaatgatt tccttttatc tcgtacttct tcaaccaaat taaattctcg
     4681 gagtctcatt atagcatcct gttcatcttt attatacggt tcacgatggc gtctcaacct
     4741 aagataggaa gctggccgat atgcattcat gattttaaaa aaggaggacc tttctatctc
     4801 gcgaagtttt tccacacctt catatgtcga tatttctgct aaatatctcg aatagttcaa
     4861 tattgcacta tatcacagat attttcatca ataagtattc ttgcatgagt tgccatatat
     4921 tgaaaacttt actgtggtta cattactccg gcattctaaa cattagaagc acaaggcact
     4981 catccaagat atccgaggca cttatccata tgaagattct aaatcggcat tacaaaattt
     5041 caaacctccc gtatatagtc tcatcattct ggacgtacta atgaaaggac tagatggttt
     5101 tgagctttat aataatatga gaaatataga cgagaatgtt caagtatgct ttataacagc
     5161 atcaaacaca tttttcgaaa aatacaagaa actatatcct ggaatcgaaa aagagtgttt
     5221 catacaaaag ccaactacga ttaagaaact agcaactatt atagattcaa tcctcgaaaa
     5281 tcgaaggcaa gcctaatgat aattcaacaa gattacctcg ttttttaccc ttccccgaaa
     5341 agtgtgtatt attcttatta ctgaatttct tattatgtaa ttcatgcttt tggtgtgcat
     5401 catatcttaa cttgagaaga agttttggtg ttattgaatg tccttcttgc aaagaaaata
     5461 taataaaacg gatgccactt tctgcaaagc gacgacgttt attgaagttg cggagacaag
     5521 aaatgataac agtaattata atttgcagaa tccaattact atgactaagt gaatccaaaa
     5581 aggtcaaaac attcagatat agttagattg atttcttttt gattattgtg atactcaaaa
     5641 taaaagatga atattatata tgactacaga agataattct gatcaaaata acaaatcact
     5701 tggaatgcgt aatgtcgttg caatgggttt tgtaagtttt tttaccgact tttcaacaga
     5761 aatgatcctg ggcatactgc ctctctttat agtatccaat cttggagctt ctagagcaat
     5821 tctcggagct attgaaggtt cagccgaact taccagttat gcctccagat tggtttctgg
     5881 ttcattatct gataaattag gaaaaagaaa aatttttgtt cttgctggct atgggctttc
     5941 aacaattagt aagccgtttt ttgcagtagc tacaggatgg tttgaggctt ttattgttag
     6001 agctgctgat agagtcggca aaggggtcag aactgcaccg cgtgatgcac tgatagccga
     6061 ttctattcct gagtctagtt ctggcaaggc ttttggaata cacagaacgc ttgatcaagc
     6121 cggagcaata gttggaccag tggtggcttt tgcgttactt cagataatag gtatccgtgg
     6181 gatattcctc ttctccctaa ttcctggtgc tattgcagtt gtcattctta tatttatcgt
     6241 aaaagatgta gcagcaaaaa gacgcataaa tacaacaatg ctttcaaatg taggtaatgt
     6301 cttaaaaggg aacagaccct ttgttcttct gctcgttata tcaggtatat ttagccttgg
     6361 tgctttcaat tattcatttg ttcttctaaa agcatccggt cttggaataa acaaaaatgt
     6421 aataccacta gtgtatgctg tgatcaacgt agcctacact attgttagct ttcctgccgg
     6481 tctattagca gacaaaattg gtaaagaaga agtgttgata ataggatatg caatttttgc
     6541 attctctact ttgcttatgg ttgaatttac tggaaactcg ctttatgcat atttgttggc
     6601 agctgtattt ggaacgtata tgggaatttc agaaactcta cagcgtgcaa taataccaag
     6661 atatatcgca tccgaattac gtggtactgc atatggagta tacaatttag ttgttggctc
     6721 aggctttttt gcaagtaata tcgtatttgg ttttttgtgg gacaactaca atctaaattt
     6781 tgcaagctat tatagcgtga ccttcgcatt agccgggata attggaatgt tggcatttat
     6841 aagaaagttt ccagtaagct cagcgtcgtt gagaccttag aatagcaaaa ttctaaatcg
     6901 tttttcatgt gatgacagtt ctttatcgtg attaaaatgt taaaagaatt acctgggaat
     6961 tgaaggacca gatacagtct gcctttctga agtgcaaggt caaacaaacg aagtggagca
     7021 gcaataagtc cacgttggat aaactatcct attgtttata taatagatgt ttgggtaaga
     7081 ggtaaatgtc aacaattagc aaacagatta cgaaagagac taccaatgta ccaaagatag
     7141 cagtaggcat actagccgga atcttggtat ttggaatgtt tgtagtagga tatgaccaag
     7201 gacagttggc acaggcagta ttatctccat ttggaattca tccaaatcat gctcagttaa
     7261 tgcttctcca tgaattcaac cacgatctga gacatgctgc aggatttccg tgccactaat
     7321 attaacagta ttaaaagcct caactgacaa aaagtgaaaa cgctaacctt ccttgctatt
     7381 actcttcttt ctggggcaat tgcaggaact cttcttggct tgattaacca agttatagta
     7441 gaaccctata ttgacaaagc tataggcatt gagacacaac gagagatcaa tgctggtaaa
     7501 attatcgatc cttcacaaca attacaatat cgtatgtggc aaaagggggg agaagtagct
     7561 gctagtacta tatacggtat atcgctgtca gcattatttg gaatagtctt tgtatacagt
     7621 aggaattcgt tgcctggttc taacaacaaa aagaaagcgc tggttctgac gggtattatg
     7681 ttcgttgtat tattcttgat tcctactctt aaatatccag ccaatccacc tgccgtggga
     7741 aatccagata caatatacta cagagagagc ttgtatgttg gatttatagc catctctgga
     7801 tttagtagct tg
//
LOCUS       DAOR01000335           13500 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_12867, whole genome shotgun sequence.
ACCESSION   DAOR01000335 DAOR01000000
VERSION     DAOR01000335.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13500)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13500)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13500
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_12867"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         4234 a         2476 c         2428 g         4362 t
ORIGIN      
        1 caataataga ttcaataata gatttaacat caatgaaaat gtctgtcttt acatcagaaa
       61 tgccttcatt atttgctata tctcttactt tctcaaacca gaattcgacc atatttttta
      121 tatcttcata gtattgacct tttgtagata ctgcagaagt tgcatatcca cacggtatac
      181 ggggagtaac aacgtggata caaaatagct ccgcattctc gtcctttgca attcttgttg
      241 catatttagc ggcatttatg gagcctttac atttcttgca agggatgttt gttggccttt
      301 ccttcatttt tgaaaattta tcaagtacct aaacgcataa ccgcacctac aggcttgctt
      361 gcatgccaaa ttctattata atcacggttt ttatcattcg tcaaaattgt caacgatatg
      421 gaacgatcag taaagtgtta taagtcgtat tttattgtat ttgaggtcga gattttgatt
      481 atgatataaa tgccgataaa atgtctttta ttatactcga caaaccagat ctctatttga
      541 tgctgttttc cggatttcca tcatatagta gtaagataca agaattgtga tggattaatc
      601 aattatgtaa gaataaaaaa cgtatcccga aacctatcga aataattacg gtaatactat
      661 taattctcct ctcattgttg gctgatgata cttacagtaa aattggtatg caccatcttg
      721 agctgcttta aaagtgatta ctgaatgttg tccaggagct aaatctttgt caatgttata
      781 tggtacccca atagtgaatg aatgtctatc gccatttggt ggcttctcaa gattgtagaa
      841 aattacgtta acagtgtctc ctttgtttac tgctatctcc gatggagaaa atgaatctgg
      901 tggaatttgt aactgtgttt gattaatatc tccttgcgct gatgtaaata tgtaaaatgt
      961 cttatttgtt gactgaccaa gtatggctgt tgacataatt ccaatattag tttgattcat
     1021 agtcatgggt atcgattgtt gttgagcagc agcaatatga ggatcatttc caccaacaat
     1081 agctcctagt cttgtaagag acaataaagg cgatgcaagg gttgccgagg caataacgaa
     1141 tgtagctatt aaaacagttt tgattgtatt attattattt ttttctggtt tcatcttatt
     1201 atcgttaata atcgattgtt actttatata actatctact caaaactatg gatcccaact
     1261 acataataga attggtgtat ctatctgcgc cgcacctgat taaacgataa acaaattata
     1321 ctatatactt ttcaatctag aattaatata atagtatggt cgatgttaag taacaggaaa
     1381 aagagttgtt gttaccctgc ttgcgacaat acaataccat agattagtgt atttccaaag
     1441 tgattttgta ctctgattgg atcagctcgt agactgatag tatggccgtt cattatcttt
     1501 atgcttactg gtacattcag atgtggccca tccttcatag ttattgtagc tgtaccattg
     1561 aacgtaactg atgagtaatg gttcgtattt gaactattgg cactcgttag cttaaagtct
     1621 gacattgaat gctggtgtaa tgctgtgcca tttagatgta ccatcgtcag gatcgtactg
     1681 aatgtggcta aggctggttg gactttcttt agttcaatat gtggtttaaa cagcaacatc
     1741 tgccattcac cgctaactat ccatgctggc ttaccgcttg catcattttg caaacttgac
     1801 ataattccta cagcgttagt gaaatttaat ttatcaaccg cattgttcaa cgagttggtt
     1861 gtctgtggtg ttgtaatgct agttgaattc gacgtatttg catttccttt aaaacttgat
     1921 tgtgctaata ccgtgtgatc ataagaagta ctaaaagctc cgctaagtaa tgtcgcaaat
     1981 ataccaagtg caatcacgag tcttctacta atcatcaatt acgtatccgc atacggtgtg
     2041 catccttatt aagatttgtt gattaaaaat agcaatatgc cattacttgc ttgcttagat
     2101 gatggaggac aatagcattc tatttattca cgaactataa aattacaatg ttgaaaaaaa
     2161 gctgattttt attttgtgtt taatgcatta ccaatcatca tattcagcaa aataaaccgt
     2221 ctaggctagg gagctgaggt ttcaaatcgt tattgtttgc caactgtgct aagaatcgga
     2281 actatattat tgcatttact ctttctatta tctcttcatt ttcaattggc ttttgaataa
     2341 agtgtctttg gccttcttta ggaaagactt cttttttaaa cgcatcgtac tcttgtaagt
     2401 cggtcagagc tgtcagaaaa agagccttga tattaagatc tattttcttc atttgtgcat
     2461 ataattgaaa gccattcatt accggcattt tgatatccag aattaataga tcatacgtgc
     2521 tgcttttgaa attctccagc gccaaagtag gatcaacaaa cgtatcaact ttaaatccat
     2581 taccttctaa aacctgcttt agtgctatac agatatctgg ttcatcatct actatcaaaa
     2641 ttttgttctt attagtctct ttgctattga taatcatact atatattggg tcaatatgag
     2701 tttaagtatt gatcgttgtt attacgtaca ttatagaaga ttaacaaccc ccccgtttac
     2761 ataaaattta cctgaaggaa ccaaaattct ccctttagag ctttaaataa gctccgtttt
     2821 catattctct cgttctatgt ctctagaccg ttctactgca agttcatacc tcgactttct
     2881 attagtcttt ctagcagatt ctgcttcata aatgtcctgt cgaagccaaa atatcctaat
     2941 acagtttctg cagaaattaa tatttgaaga ggtaactgaa cctttgcacc tcaatatgaa
     3001 ttcgtcaact ggactaccaa agtttacgca attaagagag gtgttattta tttttcttct
     3061 ccaacataac cttcaccgtc ttgccaatcc aagatccagg taggtatatt ctgcctgagc
     3121 tgtcagagct ccttccaact cgtttgcgaa tgactgatgt tgtttttgat cttgattttc
     3181 gtcgcttgcg tttgttcatt cgtcctcctc cttacttttt acaataaccc tttttgttta
     3241 gccaatgggg gacaaggata tgaatatctg taccatgctt tgttactttg ccattgtttt
     3301 tcatttataa ataagtgaca tagagatgtt attaaatttt gtatataaat cctctcttct
     3361 tccgtcgtaa tatgaagtcc tgttcttaaa gcatttgaaa attgggccaa aattgtctta
     3421 cgtcttaaga tcccatcatg ccaggaattt taaggggagt tctataatcc ttagtaacac
     3481 ttaagccatg atctgctgtt acagtcactg atttaagtgt agaattttct ggcggtgaaa
     3541 gcatagcatt ttcacccggc gaaagtgaag tcaacttatc tactttgcca ccataattga
     3601 ctacaatatt tgtcaaagga agtttgcccg tatttgtaat tgtaactctt gccgtactga
     3661 aaagatactg attgtctttt atcggatcta cactaatagc ataatcttgt gtggctactg
     3721 caaagacaga gaacaataaa aataatgtaa ccactgcaat tcctccacct accgctgaga
     3781 agagaaagac gcgcttcact aggaggtatg aaatgagcac gatattttat gcttttgatt
     3841 attcacacaa tttatattat attttcgact tttgaccata aattacaaga aattttatat
     3901 ttcgttttat aatctatttc aaaatagatc aactcattag accgacatag aaattggtat
     3961 gaaatgcttt tttagactga cattgtctac attataaagt agaataattt gatttttctt
     4021 atgtaacgaa tccgattctc tcagagtatt gcttctcact atcagaatca tccctacccg
     4081 gaagagagaa ttgctagaaa ccctatctct gatgaatctg acaggtagca acttgaagta
     4141 tacgatgcat taagagaagt cattagtaac tagtaacggc gggcactcta ggctggattt
     4201 gcgcttggac ggtgaagatt acaaattcat attgtggtat attagcgatc gtcgcaggat
     4261 aggatgtttt tgtcaggaac cgctccgatg atgatttttt cttcctaccg ttctttgcaa
     4321 aaataaatac taactgatca aagacgttgc tttcggaatc atatccatga agatgtttgg
     4381 aaggaggagc tgaagattgt tccaagtatg ataatgactc gtagaactat ttctttgtct
     4441 ttggtctagt tgtcttcgtc ttcttttttc ctgtccttgg ttctatgcct tgcttacgtt
     4501 ctactgatgc aacataggca ttcgctctat cttttgatat cccattcttt ttctggatcg
     4561 ctgcagatgc atctgaaaat tttcgtattt cttttttcct aattttaccg gcatgctact
     4621 aatttttact tatctaacta taagcttttc tttattaact gaatctggac tcgctgagta
     4681 atttctaatt tttaatctat gataataaat ttgtatttgt acggacgctg agaaatttga
     4741 tataacaact cttctatttt tggcaatatg gctatactaa aatttcagta caatggttat
     4801 taactattac gctaaggaat aaagtatgtt gtcattatca tgtcgcgaca taggaattat
     4861 ggactgcgaa tatgttgcta gaggagaaac ggaagaagaa ttgtggggag aaggtgtaga
     4921 gcacataata aaagtgcatg gcacagaaga tgcagatata actccacaat ttaaagagag
     4981 ttataaacgg tacatcaaat attcttaaaa atcagacata aatatcgctg aagttttaaa
     5041 accagtattt agaccaataa cgggtcatct gactacggga acttcagcaa ataataaata
     5101 agaataagat aatgatgatt cggaagtctc tgctgtcagt tctcaacacg acgggagtag
     5161 acaaagatga gaaaacttca atatgatttc gtggaaagta tagccagccc aatatttgta
     5221 gcagtatcct cagctatgga catatatttt tcaatatcat tcaattatgc tttttatttg
     5281 aaaggtcata atatagtata cagcttctac tcagctttct gcttatagaa taatataccc
     5341 aacttagaag tataatagaa attatcctga gtggcatttg ataattagtc aaaaatgtcg
     5401 atgctgtcac acctaatcca ccaaaaatcg atatgaggag aaatcccagc gaagaacagt
     5461 ttgcacaagt acttgagagc acactcagag atgggcctga aaataatgac aggattccca
     5521 tcttcagtct atttgaatgt cttatgacat acacattcat acttattatg acacccacaa
     5581 ggacggcagt tatgttcgaa agaataaatc ctcctactgc atcctcaggt agatagaatg
     5641 taactattgg agaaaagaaa agcagctgac aaaaacatta aaaataatcc agaaaatcgt
     5701 cgtagcagct ccatttaata taacgtatgg ccacctagaa aatactaatt tagttgaagt
     5761 agtaattgat attattttta ttatctttat taatcttaaa ctattcaaac tcatatcctc
     5821 atccgcttcc aatatccttg tcaatgattg ctctaaacta cgggaatgga tatgcaccag
     5881 gtagagtttg ggaattagaa ccatcacttt tctcaatcat aaaagtaggt gttccttgga
     5941 ggcccaatga agtagcaaaa gtaaagccct tttgcacaaa tgacagatat ttttgactat
     6001 caaaacagga attgaacttt tgcatatcta ttcccggtat ttgagaagaa aacttctcga
     6061 gattctcctt gctggcccaa ccaaagtttg caggtccttg gtttttgaac aaaatatcat
     6121 aatagttccg gaattttcct tgatcattaa cgcattgagc tgccatggca gcaggagtag
     6181 aatctggacc atatattgga aaatacttta gaatattcta ggcattgacg taaaggtatt
     6241 caaggatgga gagcataggt atattcggat gaaaatctct tctgctttct ccttgaaggc
     6301 atgacgtgtt attttaccta atcctgaagc tggatcaagg tgaactttca gtatatataa
     6361 agaaaatgtc ggacaaccaa tacagcaata aatcaccaag aaatcctaca atagatggtt
     6421 ttatcatttc agagttttta cattttctcc atattgctaa tgttggtttg agtgtcattg
     6481 cattattggg tgcaagtgtt ttgtatcatc tgccccggta cttaaaaggg ttgcataata
     6541 ggtaatccta agccgataca aaacgtttat ccaacctacc tccatcgatc aaataaagta
     6601 agcatattta cgaaaaccta agtcacaggc tcaacctact tcgacagaaa gcggatgaaa
     6661 gaacttaggc tcaatatgac tgcctagtta ttgatcgtta aatctgggag cataaagtgg
     6721 tatcttacgt agaaataatg ctaaaagtgt aaatcgtttg atagatagca ggaattgtta
     6781 tgagctcatt caaaaaggat ttgtaattac aaaaaataat ttcagtcttc accacgatcg
     6841 tgtcaaaact ctcgaagaga caatctctag agacaaagga gaaatgatat tttctcctgt
     6901 cgtttgtcta catgaaaatg ttgatgtgct tgattatgat gatgagtacg caaatctaat
     6961 tcttaagcat aatctaagtt atgaaacagt cgtctcttat ctgtagttca aactatggac
     7021 aaaggactac tcccaatggt gatcgaaaaa gtactcaaac ggcgtcttta tttcaaaaat
     7081 attaaacaaa aattaaatat taatagttca gaatggcttt ggtgcgagca gcgactagct
     7141 gaaatatatg agtcgtaaaa aattgcaatc aagaatagca tctttcgaac acagcacagg
     7201 taagaaatcg acgtgtcttt ctcaatttgt aaaggaggaa tgttggttct ttcaggtaga
     7261 ttttctgcaa aatcggaatc tcccatagtg tatgcaatag ttgaccgagc aataaactat
     7321 tgttattcga catatgaatt tctcttcgaa tgaggcgtga tcaagttttc agtagtattc
     7381 cacaagtagg gcattatgct tattgaattg catcacaaag cctcgacatt atttgttgat
     7441 gttgactaga ggtttctgat ggcatttgca gatatataca cagaaacttg tcttttgttt
     7501 gtgttagtgg agtgacagcc aagagcatct taccattatt atcctcttcc tgattggcta
     7561 tttcagttat ttcgaaatga gttttattgg aggtccaatc caaccagcag attgttgccc
     7621 tattgtttag agatggaatc aaataatggt gataaaatga gtaacctctt ccggtccatt
     7681 tagaactgtt agccattttc gtagaatctt cgcgtccgtg tccgtggatt ctcctgattt
     7741 taccagttac catttttcca ttagaatcga cgacagctgc aaacttgact gctttagtag
     7801 catacaatga agtcaaacag atttcctcaa agaaatcgag ttcttccatt gatcagtgac
     7861 tctatctaaa ctgggtaata ttaatcattt atctactgat gtaagatagc tgtagttttt
     7921 gtatacaatt tccttaaata atataaatac tatatctcat acgatcaccg taggaaacga
     7981 tagatattga cttgcaaaga catatgctct cgtcataaag caccaaaacc agttggtagt
     8041 ggtcgctatt ctactggaca gaaacgatgt caagtatgtg agatcttctt aaagtgggac
     8101 ggattatggt gtccgtgctg tggatatcga ctaagaacaa aaccacgcaa cttaaaatac
     8161 aaagcgaaat tgagatcaac aaaggaaata gagaaatcta gactactact atcatcgtca
     8221 tatcattagc aacatttcga tatctgtatg caggctggaa tatcagaaga acaatgatat
     8281 aagaataata aacgatacga gtgatttaga gaccagaata aaaaaaggcc tcatcataat
     8341 actatagttg ttgctgacgg ctgtgttcct ttatcaagaa gtagcatcta aaccagccac
     8401 tcgcaatcct tgcaacctgt tcaagtttac ctggttcttc aaatagatgt gtagcgcctg
     8461 aaataactgt gagcttcttc ttttcagctt taatgccttt tagtgcgcta tcattaagtt
     8521 tgaggacttc ctgatcttct gcaccgacaa ggaagagtgt aggcgcttga acttttctca
     8581 atataccctt tccggccaat tctggtctac caccacgcga aacaacagcg ctaacaattt
     8641 caggtctttc ggacgctgaa accaacgccg ctgcagcacc tgtgctagca ccaaataggc
     8701 ctacagtaag attcttggtg tcaggattat ttgcaaccca gtctacagca gaagtaagtc
     8761 gctttgatag taagccaata tcaaatctga gctctctcgt ctggtcatcg attttctctt
     8821 cttctggtgt aagaaggtct aagagtaaag tggtgagacc gttctcattg agttttcccg
     8881 ccactaattg atttcttggg ctatgcctac cactaccgct tccatgggca aagagaacta
     8941 ttcctgctga attttgcgga acatgtagat tagcatcgag gaagatcttc tctttttggc
     9001 ctaaaggttc gttttgcctg tctatgggaa ttttgaccaa ccccaactca gcttcatggt
     9061 tgatagcacc gtccttaatc aaaaccatat cttcctaata cgatctagct ttgcatgata
     9121 tatttgtaaa caagttgcag ttctcatcag tataggtttt gttatctgca ggagcggtta
     9181 tgtcattcta agagcaggaa agcgtggtag aaatcgaggc tagaccacta attcctatat
     9241 cactcgaatt ctaatgttag cttcccactc cctgctagga gcttcaactc tttttggaaa
     9301 aagtattcct ttccttgctt gctatgagac tgagagaata taaaactaga ttcctgggtt
     9361 caggtattgt ttataataaa gcgttctgct cacgatactc acacaaaggc gacaagcatt
     9421 aagacatgac agaatggcag aagagactgt tcttagtaca ttttcgggac cttagagcac
     9481 tttagtcttt tctgtagtat taacgtctga ttctatcgcg aattcaagag cataaagaac
     9541 atttttcgca aatcagtcat atgatgggag aaatgagatc tcttatatga cagcgatatt
     9601 ttattgatgt tttcatccat cttcgtgggc ttgagtatag tataggaaaa cgtaggtggt
     9661 agtattacga ttgcgcctct ctttcgtagt catcgtagcc ttccccaata tcatcctagt
     9721 ttctggtaat gctcaattcc agtacctccg tcgcagtccg tctagcatca tcactatcaa
     9781 cttctattgt ccccagctat cgtcatcacc cttgccaatt catcgatata ctttgcttca
     9841 ttgaaaagaa aaattatgaa tcaaaatatt tttcaattac cctcgacata ttgagcttag
     9901 ccacatcttt atcggccaat acttcgggat gcttttgttt caaatgtctt aacgcaaatc
     9961 taacatcatc gccacataat ttgcattttg cctttccaaa catagagtta ttgtaataag
    10021 aattagttaa aaagtgctcg atcatagcaa acatcttctt accggatgtt aaagatgggt
    10081 tgaaagaaaa tctattgcct tgaaattgtg ggttggttat tctttcttta atgcaaaaaa
    10141 gatgaaagta taattacggc cgcctttcca atgtacaact ttatcttcat atgtaaaatc
    10201 aattgattct gtaagagtaa aaccattgtg ttctgcaact ttaattacat ccttctcaat
    10261 ctcttcctta ctgaaaacgt tattaggcga catcttacga atatctttgg aaataatttt
    10321 gttgactaat ttattgggcc aatagtcgct cgatgtcaaa agcattccac ctttttgtag
    10381 tattctattc atttccttga aatagttttg aatattaaca ccatgttcaa ttaccgataa
    10441 ggatgtaata taatcaaaca tattattttg gaagttagtt ttttcgaggt cttgaatcga
    10501 gatggcaaat atcttatttt cgtacgtttc tattataggc ttgtaatttc ttttgtaaaa
    10561 agagtgtact attttcatta atgcaggata cattattgta tttagaaata agtcgcaccc
    10621 atagagattc ttaaagccta atcttcgaag cataggtaag ataggagaat cgttacatcc
    10681 gacatcaaga atatatgagt ttctatctgc tccatttatt gtatcaatca ttttgcgagt
    10741 atcccatgat tttactcggt catggtgcgg aaacagacca agggaacgaa gatgatctat
    10801 agcatcatcc acttcaaatg agtttttcag aactgaattt aattccattt tttaccagca
    10861 aaattgcttt ctaataagta atttaaactt atgaaacata taggggaaat taaaaatgat
    10921 ctttattatt taacaggtgc atagtctccg accaaacaga attagaagtt gaaatcaaat
    10981 gcccactagg tctcagttgc aattttggga tgtgctcaaa aagccgaagg gtctataaga
    11041 ttaatgcaat agtcacactc gaactgagta cttgtggata tgctcagacc tgcaaaatcc
    11101 caaactacca tgggatgact tacggaatcg gcttaaatat ccagcaaccg ggaggagacg
    11161 gcatggaagt gaatatctac tatatatgaa tgctatagat aatgaaggaa cactcgataa
    11221 ggaagcaagg tcttcgctta agatataaac aaatttcact actaattgat tattctcaac
    11281 acgctaccac taccctatta cagaccttgt ctttaccata tctcaatgaa ggttaaaact
    11341 agtcatacta gcatcacaga tcaggacgtc tacatccttc tcagtaaaat tcctcctggc
    11401 aaagtttcta cttacggaga tattgcgaag gcattgggac atcctaaggc cgctagggca
    11461 ataggtagaa tcattgctaa taatcctaac ccaatatcga taccatgtca ccgggttgtc
    11521 aaatcaaatg gcgagatagg tggtttcgca tatggcgaac aaaggaaaag agagatactt
    11581 gaaaaggaag gtattaaatt ccaaaataga attgtagaaa actttgaaga gcaaagatta
    11641 tcgttttaaa taaaactaaa tccacgctga actagcgaaa tactaaacca taaatctgct
    11701 tttggataaa gatatggcta agatagctcc acttttgccc ccgcaaaaca ggcgatggag
    11761 ggctatattc taagtatatt gatgaagacc tcaggggtat tctagatttc actaccaatt
    11821 gtgatagaat gacacaagaa atgaccaccc agatcagagc tgaggcggca cgtgatatca
    11881 cgacgcagct gccggaagct aaatctcatt tgtaacaagc aagaggggtg cagcatgggg
    11941 ctcatagcca tgtcctccac tttctagctg gtccagttgc agtttgatat atgctttttt
    12001 taaatctagt tatatcataa ttattataat aaagtatggt aataagcaga aatcatttaa
    12061 tatctcaaga aatatattaa gaaaattaga atattgtgtg aaaggataat ttttagatcg
    12121 agcgcactgc cagccatttc attcagaact tagtccgagc gtcatatgca ttacctacct
    12181 aacggctgac tgggttcatc aataataact agttcaacct acatcagttt aatattggtg
    12241 aatactatac aaagttgtat agtagggatg gaaccgcaga aatataattt taacagcttt
    12301 tacagataca ttattgcaaa ttctctattc actactagac aaatagatat tatttctaga
    12361 agattggaga ataggggcac tatagaaaat ataagttctg gtgcctatta tagacaggta
    12421 aggcaaagta gaaccaagat agtaagattg ttatatagta taattctcct gaaatgcgtt
    12481 ggtgctatcg atcacgaaac atttttcgcg attgaaaaaa tggcatcaca aatagaagtg
    12541 atgtttgatc aaaagacgag tgataattca agagctgaaa gtgtgatatc cgtgattgag
    12601 cagttagtaa aaagaatgtg caaagtgtga atcaatatta ttctacaaga ttaaatcacg
    12661 gcttattact tctcaatgtg atatgtctct tgaaatgctg aatttaagtt tgaatcttgt
    12721 gatatatatt gtgacttcat ttgtcatcgg catggcaggt gtgatatttt atataaatct
    12781 gaaagcactc cacaagaaat ctactaacca gaccgataat agtgttgttg aggctgtcat
    12841 atctgaatat acacgcaggc tgcaggaatt tgagagaata attggcgaat taaaagtcga
    12901 aattgataca gtagaattgc gagtgctgca acaccccaca tcgcagactt ccgacgatac
    12961 cagtgatatg acccgcgact cctctacgga tgcgagaaca gtatcacaag ctaaatcaca
    13021 actgcaagaa catcactcac caacatctga tgtgatggaa tcgacaacta tcacgccgcc
    13081 atcgattgtc acatccgtga aaagcaatcc tactctgcat catagcactt cagactatat
    13141 attgaaatta ttagttgaga aatccagaac ttcaagagaa atccagaatg ctataggaaa
    13201 gacacgagaa cacacatcac gccttatgag aaggctttac gaaagtaatt tagtcagcag
    13261 ggacagtaat aataagccat tcaaatatta tattactgac caaggacgta gagaattaat
    13321 attccatact acaactggtg agtcagaggc gcccgatatg aatgttcatt ctggttcgca
    13381 ggattctcaa ctgaatgtta ctgtccatta gtagacgctg tctttgaatt tcttcttctt
    13441 tattccctgt tggacatacc tctccgtttg ataaactaac acttaacact taacacttaa
//
LOCUS       DAOR01000336           12316 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_10740, whole genome shotgun sequence.
ACCESSION   DAOR01000336 DAOR01000000
VERSION     DAOR01000336.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12316)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12316)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12316
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_10740"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    6210..6487
                     /estimated_length=278
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9150..9383
                     /estimated_length=234
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3416 a         2508 c         2363 g         3517 t
ORIGIN      
        1 atcctctgcc atgcacaata gaggattgta tagcagaatt ttgttctaaa gagtgtgcaa
       61 aaaatcatca tgctatgcga catctgcgcc actgattaat tatcaatgat atctgtcccg
      121 acaacaaatg gccatgatca tagatactgt gtgtttgttt gttcataaat atctgggtac
      181 tatatcgtca tacacatcac tcgtttttat ctttgactag atccaatgtt atcatcctta
      241 tcctttcagc atcacctctg aattgagtta ggcatcgggt agaatactcc gcagggggtc
      301 cgtccatact atctctaata gtaaatccaa gagatcgaaa aatttgtgaa agccattgaa
      361 cccaggtatt gtaggcttca tctgaaactg gttcaagaag atcatcggtt atttcggtaa
      421 caaggcggag tagtcctgca ggttttaatt ttgcatacac gactttgtag aacgattgcc
      481 agctattctg atttgtcctg tcaatgtaag caggatcagg tagcacatcg agaatctgat
      541 caatagagcc atctggaaac ataggaagaa tctgttcaaa agatgcgttc aataaaataa
      601 catttttgtc atttttcata agcgattctg cagcttcgaa ttgcttccta tcaagctcaa
      661 tgccgatgta tgtgacgctc gacgtgttag tatctagatt gatatcatgg tcctgctcta
      721 tcagtttctt taagagacga ccatcgtcca ttccaaattc gattatcact tttgagggac
      781 tttttaaggc aaagcttaac ctcattaatt tgtcagaaga cataaggtac gccagtcgat
      841 accgatagat ttctgtgagc ccctctcagt cttatgaatc tttgagtagc tcgccttcaa
      901 gaagctgctg aaccaactgc actacttctt gttcaacctg ttccctaggc aaagcgagct
      961 tacctgaaaa ctgatctgtg agttgattga ttgtctttgt gccgtcacaa gtgtcccaaa
     1021 aatctagaat cgcttgattt accataaatc cttgttcttt atcatttagt agggctagcg
     1081 atccatcttc ctgtttagtt agtctgcctt tcttcgaagg ttgatatttt tcatagtcta
     1141 cttggaatcg tagcttcggt ttaccaaatc ccattttctc gcgcgctccc ggatccattt
     1201 tttcaatact ccagggagga tcccagacta tctgcacatc gatgttacct atgccattaa
     1261 tcttccccac atatcttttc acgtcgctga caagagtgtc atgaagcgga caaccacgtg
     1321 ttgtcatggt catcttaata tcgacattat tgttttcaga tctaacttgt actccgtata
     1381 ctagcccgag ttctactatg ttaacaggaa tctcaggatc catgcatttt cggagtgcag
     1441 aatatacctc atcctcagta atttgctgcg ctgacatacg acacggctat tgggcactta
     1501 tgcgataaat actttcccta ctgtctatac aaacatagat atcactttta acccaataag
     1561 atcttcagat ttttctcaaa aggtggcctt aaaactcctt tttctgtaat aatacccgaa
     1621 atcaattcag gaggagttag atcgaatgcc gggttaaaca ctcggacacc cagaggtaca
     1681 attcttgtcc cagctatttc ggttacctct tcaattgggc gttcttcaat aacgatgtct
     1741 gccgggttac tttcgaaatc aaatgtagag agaggagccg ccacgtaaaa aggtatttta
     1801 tgttgatttg caatcaacgc cacctgatat gttcctatct tgttgaaaac atgtccagtg
     1861 cgcaaaactc ggtctgcacc aactataaca cgcttaatca tctgacgaga cattacatgt
     1921 cctaccgctg tgtctggaat cagactgaca tcaatcccgt catgctgtag ctcaaatgca
     1981 gtcaatcttg aaccttgcat aacagggcgg gtttcagtag ctacgacact tatgtgcttg
     2041 ccggcttcgt cagccgcgcg tatgacaccc aacgcagtac cataaccgac tgttgctagc
     2101 gaacctgcgt tgcagtgtgt caaaattacg tcgccatctt ctatgagatc agcgccgttg
     2161 atgcccatct gcttgttaac ctgaatatct tcttcagaca tttttaatgc ctcttcaaga
     2221 gtcgcgtttt tcacatcggc gacatttttc tgttcccttg ctattttcat gacccgttcg
     2281 agtgcccaga agaggttgac ggctgtagga cgagtggatc tcaagatcgc ctgggccgca
     2341 ttaagatcgc taatcagttc ttccagaatt cttgagttac tgtgaattgc tgctagtgca
     2401 agtccgaaag cagcagtcac tcctattgcg ggggcgcccc ttaccaccaa attttttatg
     2461 gcacttgcaa cttgtttgta gtcatcaagt ctaacatatg tcaacatatt tggtaattta
     2521 gtctgatcaa tcaagactaa tgatccatct tcccaggcga ctgttctcag aaataccgga
     2581 ccatcttctt tgctgtcgac catggaacga accatttgta cgaaggaatt cgttaggtca
     2641 gttattataa gcttatttgt gcattgaaga gtgtttaaga taaggacccc gtcgacgcgc
     2701 atggtattat cagtgtagct tgtgttgtgt tcttttcttg tttttcctgt cccgacggta
     2761 agcgtcaaaa acggcagtat atctcaatat ttgctactgc ctattccgat agtggcatat
     2821 gaagcatggg taaaaattgt cctatccaga ataccacgtc cgtcgtagat cgggaccccg
     2881 ataagatcag gtgacaaagc acggtattca gcatggtcag ctacgatcat aatgagatct
     2941 gtgccattga gagcactagt gagattagag gacaaaatga cgttttgatg tttaagcaac
     3001 aacgggtcgc tttttaccaa cgggtcgtga acacggatct cctttacccc cattttctga
     3061 aattccttga tgacatcata agtcggagat aatcgggtat ctgaaactcc tcctctaaaa
     3121 gctaggccta gcagagtaac tgtcgcccta tccaatgact cgtcttccat ttttttcttg
     3181 ccatttagca attttactgc ctcccgtaca cagtaggcag gcatggcatc gttcacagct
     3241 cgtcccaatt tggttatatc gcaatcgact ttgaccttct ctgcagcgta aagtatgaac
     3301 tgcggataaa ctggtataca agcacctcct acacccacgc caggactgtg cagatgacag
     3361 aacggttgtg aattcgcagc atctctggac tcccaaaaat taaccccgat cttttcgcaa
     3421 aactttgcaa gttcatttgc aagcgcgatg ttaacgtcac gatacacacc ttccaacagc
     3481 ttttcagtct cagcagtctt gatgttacta acggaaagaa cgcccttttt gaatataaga
     3541 gaataaatta tttttcctaa ttctaggctt tttgaacctg cacccgatat cacggccggg
     3601 tatcgttcct cgatgtcttg tattgcacga ccttcgtaaa ttctttcagg attatatatc
     3661 atataaaaat cgttttcaac ttctagacca ctttctttct caagtattgg taacacaaga
     3721 tcttcggtag tcccaggtgg cacgctggga ttaagagcga ctacatcgcc tttttttagc
     3781 ccccttccta tcgctgatga tacctccttg acagctgtaa ggtcaggaga cctattatta
     3841 tttgaaagca gaaccggaac acaaatcatt ttaaaatatg agtcatgcga agcctttgtc
     3901 ggatctttgt atatttcgaa gcgtttcgtc gctagtccgc ttgcgaaagc atcgttgacg
     3961 cccggctctg gaacatgcga ttcacccttg cttgctctct ccaatacggt gtgggattta
     4021 tccacaccta tcacgtgggc tccggctcga agccacaccg atgccatagg tgagccaacg
     4081 tgtcccaaac cataaattgc aattttaaac tcgccagact ttattttttt agcaatttcg
     4141 gatcccgaaa aatcaatggc agaagctggt cgagcaggat aagtgaaatg ggttgacttt
     4201 tctcgtgcca tatgcttctt atctgcgcta gccatggcga cactttattt cgattaacat
     4261 attattgtat gttctgttac tagcttgcct ccatattttg ttcagatatt tcatggtgat
     4321 tttagtacgc atgaagtaat ctctatataa taggaaaggg acttatcgtt agactttcga
     4381 acattgacca agtgtatcgc tttccattct tacaaaggtg gcactggcaa aacaactctt
     4441 gccgctaatt ttgctgcttt actggcgaag aagggatacc gtgcctttct gttagacctt
     4501 gatgtttatg cacctagtct tcagtcatat tttaatacag atcctaagaa atggattaac
     4561 gactttctat acgataacgc tgaacttaaa gaagtagtac tggatttcac tccggtaata
     4621 gacaggatag gagggggtag tacaagtaat agcgcggaga ctaaagccaa ggcggagcgt
     4681 ggtcaactct gggttgcctt ttgtaatgca aagaaagaag aaatttacaa actggaagga
     4741 gggtcgccag caacgggtaa gcaagatacc tcgaaaatac atctgcttcg gagatttata
     4801 ctgttaaggg aactgctcat atctgagtat cacgcagact atgttataat cgatactagt
     4861 ccgggtataa ggtattggtc tataaatgcc cttgccgttg cggacacact tctcttgaca
     4921 cttaaaatgg gtgatctcga catcgcgggg accagaaaac tcgctgaaga aatttatggt
     4981 tcttttacaa aattcggaac caagtcattc cttctgtgga atagagtagc tgggtactgt
     5041 atcccacata atgttcatgg tgataggatg ttgtactcga cggcaagccc aagcaccgcg
     5101 acagctactt tgccaacagg atcaggaaca cccgcgaatc tcgatcatcc tgcttcaaag
     5161 tctaaatcgt cgcttctgat ggaggagcag caagcgactg aaacagatat gaagaaattt
     5221 ctaacgaatg aaactggtat ggaagtaatc tcagctatcc cgtgctattg tgatatccag
     5281 ttcagcagaa aagaattcct aaccgtccta gaacacccag agcatccttt tacaaaacaa
     5341 tttgagcgtt taattcaggc agttgaagcg gtttagggga cgagatcggc atgcacctca
     5401 agcgatttac ctgtgttaat ttcagatttg cagggtaaat gctatttgaa cgaattaggc
     5461 gtaattttca attaaggttt aaagaccggt caagtgccgc taatatactt gcagacgtta
     5521 taaagtcttc tttaaggaaa gagccaaaag agcagattct cgttttagga ataccacgtg
     5581 gcggtgtagt tactgcagat atttttgcaa gtaaattatc tattcccaat tttgatctag
     5641 ttataccaag aaaacttact gatccgtaca acaaagaatt agccataggt gcaatcatgg
     5701 aagatggtac tacctacctt gatcaggagc ggataaacaa tccattgatc tcgtttgact
     5761 atatcgaaaa agaaaaacta gaacaaattg cagaaataaa aagaagagca tcactatacc
     5821 ggggtaacgt aacacatcct catacagtac ctggagcgac tacaattttg ctcgacgatg
     5881 gcgccgcgac tggtgctact gttattgctg ccgctcgatg gataagaaag cagtacaaac
     5941 ccaggcattt catcgttgct ctacccgtcg tcccgaaacc gactctaaag ttgttaggac
     6001 aggaatgtga ctcagttgaa gcggttatag ccccaggcaa ttttcactct gttgggcaat
     6061 actatgagga tttcaatccg gtgactgatg acgaggtcat ggatataatg aggaaaagaa
     6121 acatgtcgtc tcaataatat ctatagaatt attacttgat ctaaaggtaa tatagacaaa
     6181 ctaactagct aactcgagca acagtttaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnaaa tcttgtaaga agcaacttta gatatatttt acagatgggg ataactcggg
     6541 tggtagtagg gaacgctctt aaaattgcta atttagcctt tcatttcttt ttttacactc
     6601 gagaatatat tatgcagcac tgttacgtag tttgaaatca actcaccaca ttccatctca
     6661 tcccgatgct tttcaagatc atttccaaaa gccattgtca aggatttggg gagtttgaga
     6721 tgttcattca tgtagattaa aagtgacaaa tacatcaatt tcatcatgtc gtcgttatta
     6781 tcctctttca aattcatgct agtaatgaca ttggagacta ttattgcaaa tgaagaagat
     6841 aaaactgcct tattgctttc tcgatcatga acatccatga acttaattgc ttccatataa
     6901 agcaaatctt cgattgcaga tgccatcttt tgagtactgg tatctttatc gcctccacta
     6961 tcgctcttgt cgctgctgtt gcttccctcg ccgcttgaca tattatatgt taatatgatt
     7021 attgattttt catgttattt atcaatttgc gatagccctt aataaaatcg ggatctacca
     7081 gcccctgtac taccagttgt ctccctgttg acaacttaat atccattgcc accgcgctat
     7141 atgggatttc aaactgttct attttttctg gatcaaaact ctcaaaatat ttgttgcaaa
     7201 ttccttcgga acatgagaat gccataactt tgtcgatctt gcgattccca gaccctcctc
     7261 ctcttccacc agcagaacca gctgattcct gaaccaataa aatgaaaatg tcattttgat
     7321 tattgttgct caagtcgata tcattttttg ccacaaccag ttttcgctga ggcatttcaa
     7381 ggacgtcttc gacagattgt tctatcgcca tattgttacc cgtgttttac aatattaata
     7441 ttattattat tattattata aattgtgcaa ccaagtgtac tgagatcatg actctttcta
     7501 cccttactat cagagttcaa aaatcaggat ccttcactca ctcgtgcgtt tttcctgaag
     7561 gataaatctt acttggtgcc agggcccgta agctaccttg acggaatgct tagttacgat
     7621 caacacagcg tcctctctga tcaatgtatg gtttctgtca ggttattcag ctattcagtt
     7681 tccatgagtt cagtccaaca tctcgtacaa aagtcattat cctcaactag tttctgctcc
     7741 aatgtcagtg tttgaagatc gtatggatgg ttacacatta cgcagacttg catcatacta
     7801 ctattgtaca aaatggattt ttaatatttt gtatttttct ttagagtgaa tcggagttat
     7861 acttagtact agaacattcg cagtaaacct ggcttgtagc taaaggcctc aagaccgaca
     7921 aatgtagaga catctatgtc tatataatat gcaatgagca aagtcgtatt gatcgtatta
     7981 cgtcattttt cagaaaccag tatatttaag taacttcacg accgcttatt atggtgtatg
     8041 atagactatg agcaccttta tgcatttact aaagaagtat taaagctcga taagtctatc
     8101 agatgggtag gtattgctag caaatacggg gtacttctga acacagagca cagagaggga
     8161 ttgcatcccc tcatgaccga cgaggaaaat gaagaatatg ctcatctaac cgtgactagg
     8221 cataaaacta ggataaaatt tgaaccgaag actggaccct tgatatacgc tttgggcaaa
     8281 tatgaaaagc taaacagagc tactctacat ataaatgagg actactatct gctgatagca
     8341 cttgacgtag aagaaaaaaa tttcgacaac attattactg aaaaagttat tccgctgata
     8401 aagaaagaga aagaaaactt tatcacgact gacaaaacag cagcggcctg atatgataag
     8461 tccaaaccat ggttaatatt tgtcatggaa tatactattt ggatggaggc tatatttgtc
     8521 tgagtatgag gcaggaacgt tccgatgtgt agcctgcggg aaggaattct tgacgaagat
     8581 cgaggcagat aaacattatc aaacaactca cgctgaagaa gaaaccaagg ctctagaata
     8641 actcgggaat agtagtatct tacctaatga tttatttttg attttatttc attgaatgct
     8701 tcaatattag cttctaaata attggagata aagtataaca ctccatattt ttctcccatt
     8761 ttggtaatga tgttattttt ctgcagtacc aaaatgtggt gttgtatcgc cttatagtca
     8821 agccccaaag attcagagag ctggttgata ttatacggtc gctccttaag cagatcaata
     8881 atcttcattc gattatcgcc cccgcgggag cctgcaaaaa gaaaccaaag caaacgcttg
     8941 gcgttggggt cagtcatata acatcctgca tgaaataaaa tttagactca aatccattat
     9001 tcataacctg ccgccatgat tgtctaaaat gtgattgatt atatttgtta attattcaaa
     9061 gaccagccca aattgcagat tcccgcaatg cagagagtaa tgcagtcagg agagttcgtt
     9121 ggaaacattg tacctttttt ccgtagacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnngtcgcga ttgagcctaa caccataaca tttcatagtt
     9421 cttctgtgat gggcaaagga taacaatttt tcttatccaa taatattagt tacataaccg
     9481 acatactgat ttttaagatc aaggatagtt gtaagtaata taccgtgata gacctagata
     9541 agctaaagta caaagtaaac tataatcatt ccctctataa tttcaagacg aaacaatatg
     9601 aaagtaagga agcgacgtat attagttata aagaagcgag ggccaatcaa tggaaatgcg
     9661 agaaatgcca agtcatgttt gctactctta aagagttgaa atttcacaag atcgaggagc
     9721 actcgtacta aactgagcgc atgtatcctt gaaacacact caaataagga tcaaatcgac
     9781 ggcgcacagg tagaagcgcc cccgcactcc ggcgagatat ttacaaaaga acatccttct
     9841 gtcattttgg tttaaatgcc tcaggatgca cctcttgaat cccatattgg atacagaata
     9901 cactcctcgc caatcgcaag caagttgaga tgtagtgagt atcgggacac ccgattgagc
     9961 gttatcgagt cgtcctcgtt tccgcgtatt tctccttcat ctcttcgacc caagagtttt
    10021 ttagcgaata taatttctgg agcttccttg gcttgcattc tgacagcacg tacgagcata
    10081 aaagcggaaa gtagactgaa gagtccccat aaaccgttac atttcttgcg tgcgatgttt
    10141 tgatctttcc ccaactcttt ccctcctgca aagttgcccc tgacagacct cctgtgtcgg
    10201 agcgggcgtc ggtcaactgg ataagataat cttggccgcc ttctttaatc ttcaatattt
    10261 gatagagagc cggccccgtt tgctgaaaaa agtttttcgg aactccacca ccgagctcaa
    10321 cacctcccga tcttttactt ttccagagaa tcgcggctga ttctgcgacg tccaaaacga
    10381 cgttgggaaa aagctcagtt cttccctcaa acattagtgg gagcatattc agaccaattg
    10441 aagagtcgcc aattgcaggc atgtagatag gtaccttagc ttcataagcg gccactacaa
    10501 aggatttgtc aggataagag gatgtctttt ttgcagccct ccccaagatg tacgatatgt
    10561 ccgcagtgga tacagcaaag gggatgctag cgctgctatt gttgcccgtc ctcaaatcgt
    10621 tctgcactat cgtatcttgg gcttggagcg ttcctttctc cttaatgtat acatcatata
    10681 ttctcacgat ttctttagaa tacagaatat catcgtcgac gtcgaaatgt ccctgacgca
    10741 ccggtaagcc gtaagcaaaa tgtaagtcgt gataaacatt agcgcctgtt gtaacaatcc
    10801 aatcaataaa tccattcttt atcatagtgg caattgtttt gcccatgcct atcggtgtca
    10861 tagccccggc cagtgttaag cacactgtag attttgtgcg taacatgtct gccatcattc
    10921 tacctgcctc agcaagacgg cgagcattgt aacctgtgcc tgcaaaaaaa tccatgagtt
    10981 gcgaaaccgt catttcagca aagatctctg gcggctctat tttctttcct ctaaagctgt
    11041 gagctttgtc cacgtcatga taatgaaaat ccacttactt ttaagaattt taccgatacg
    11101 ctttttgcaa tagaatatag agacgtataa cattggtacg agacatgaga ggtagtaaca
    11161 aaaaacaaaa gaaagatcgc attttaaatg acgtagtaat agattattca ctagaatcta
    11221 caagtgttag ttcaatcatc aaagccatgt caacttctgg tggctttcaa tctagaaatc
    11281 tttcatacgg agtggatatt ttgagagcaa tgcgaggtga aaaacgatgc acaaaatttc
    11341 tttcctttgt aggagcattg ttatctacag gcgttcgagg aatcatacga gacatgttaa
    11401 aggacaatat gtttgactgt gtaatcacca cgtgcggtgc gctagatcat gacgtagcga
    11461 gaacatacag acggtactat gctggagatt ttaggatgga cgattctatt ctgttaaaga
    11521 aaaaaattca tcgtttagga aacgtgctag ttcctgaagt cagctatggg tcattgatag
    11581 aaaagaaggt ccagtcatgt ttgggagatt tgtatagtag tcaaaaagtg aaacaggatt
    11641 tttcagctta tgaaatcatc agccatattg gcagcactct agatgaatcg tctttccttt
    11701 attgggctca taaacgtaag attcctgtta ttgtgccagg aatagtcgac ggcgcagtag
    11761 ggaatcagat atggttgttc cagcagcagc acaaagactt caaaattgat atcttaaagg
    11821 atgaatccaa actgtcggac ctcatttatg atgcgacaaa aacaggggca tttatgatag
    11881 gcggaggtat ttcaaaacat catacgctat ggtggaatca atttagaggc ggactagatt
    11941 acgcagtata tattacatca gccccggaat gggatggcag tttaagcggt gctcttgtgt
    12001 cagaggctat atcttggggc aaggtcgcag agaaagcaaa gcaaacaaca atccatgggg
    12061 aggctaccac gttattgccc ttcatttatg ctgctctaaa agaagatggt gcaagtggaa
    12121 gataaacact actagtatga ttagtactac tagtacagta atacatggta tcgaagcaaa
    12181 ccaaaattca caaaataatt aggcacagtt tgctttagta ttttgataga cgctattctg
    12241 gggctcggcc acgagctcca atcaaatgct gcgatttatt ttattttaaa gccgtcgtgt
    12301 tcctgtgtgt cgtcgc
//
LOCUS       DAOR01000337            4138 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_68612, whole genome shotgun sequence.
ACCESSION   DAOR01000337 DAOR01000000
VERSION     DAOR01000337.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_68612"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    319..647
                     /estimated_length=329
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    890..1105
                     /estimated_length=216
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1161 a          682 c          625 g         1125 t
ORIGIN      
        1 atttgatctt acaaaaattg gtgatactag tataggcaaa atgtatcttg aaccaggatg
       61 gagctgggaa aaatgtgtaa agcctcttgt taaatctcaa agttgccaag cctctcatac
      121 acaatatgta atatcgggaa gaatcagatt caagatgaat gatgggaatg aagaagaata
      181 cggacctggc gacttagcct atattccacc tggacataag gcatgggttg tagggaatga
      241 tccatataca ggaatagaat taggaacgat ggatctattc cattcgatat agaaagtggc
      301 tacacggcaa aatataccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa gtttagccct
      661 agtaacgaag ttagtgcaaa agaaaccttg gtacataaat ccatccaatt cgtgccttgg
      721 ccactctttc ctgtttggtt ttgtgatatg aatccgaatc cctgtaccga cccagcagaa
      781 gtcatcatta gagtagccaa cgaaaccagc actattataa cagctttcga tccgttagtc
      841 ttcatagttc gcagatgcac ttttacctca cattacttag gcgatggttn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnntgaga gacgcttgtt atggtttatc tcagtaattt
     1141 cttacaccta ctttttttat ctggttcgct gcaattatct gtatattggc taaactattg
     1201 ttcaaatata cgctcatcat attcaagtgc tggctggttt gatttaattc tatagttata
     1261 ttataaagac tcatgacata cgcaaccgct atatcaaaag aaattatcgt caacgctaaa
     1321 ggcagccaga ataatgaatg gcgtcttaac gatttcagaa tataggatta cgtttaccta
     1381 aaaagaataa gaccaaaaac tgctaggtgg atgtatggca tcatattccc tttacatttg
     1441 aatcagttgt ctaaacgttt tagaataaag gttactattt ggtaagtcag tgctaaattt
     1501 atttgctata tgtttagtcc tttttcagat gattttatat cgttgaatgc atgaaatgcc
     1561 agaatatact gttaatgaat aacatgctca cagttgacca tatagtacca agacatcacg
     1621 gagtagacag aaacaatgcc agcaacctac aattgttatg ttatcgttgt cacagaataa
     1681 agaattttcg aaacatgaat aaaagcataa attctgttag cgccattcat tcaatcataa
     1741 gttgagtata acaacagaca ctaaaaaaca tgtaaagtct aaaactcgtt gtaactatag
     1801 taaatttctt cataacaata ttataatact tgattctagt attaaggtat tctcaaacat
     1861 ggtttttgac ctataaggta aggcatgact tttagatgtg taccaggcgc agcgtctttt
     1921 tcaattgttc catttgctaa aggtaacatt aacagaggaa ttaatgctag agttaaaaaa
     1981 aatggctacg actttgttag acataaccct attcctattt gtacgactaa ctggtttatc
     2041 aatggattac tagttgcgtt ttccgttaga cctttaatca cgttgtttaa ggaagaaaaa
     2101 atgaaacagc gtttttaagg gacttaagaa ggtgcggaaa ccaacgctag ataaaaatga
     2161 cttgtaaggt attcaatcgt gatgaataca aatgcagtat atgctttaaa gaactcaaag
     2221 aaaagctatc tcaaagagtc cagcatataa tccctaaaag attaggtgga tcagactctc
     2281 tcaataatct tagtcttatg tgttacaaat gtcatggctg acggaactac gtaaaccata
     2341 tgaaattaca gataatgatt ataagtatag agggaaattc tttggacgag taagttcaat
     2401 gggagcgtct aaggtagcgt acttactagt attagtaacc ctaaaaaagc atattgtgaa
     2461 tatatgcgtg tggtcaatat attcactggc agatttcaag acatagtgtc taaggaaact
     2521 aaacacaaat catcatctgg tttagagact gactattcca gttttcttcg tagggaaaaa
     2581 gttcacccaa aacaccgtgt cttgttttct gcgtcagcag gatccttttt gtttttcttt
     2641 gttacggcag ttctgctatt tccatctatc tttgcttttg gacaaaatac aagcactggg
     2701 aatatacaaa acggcgcaac aaacaatacc aattcttcgg ctaacacctc acaaccacca
     2761 attgctacga agatttctga caaagggatc tatcaagtgc agctgacttt tatctcagca
     2821 ttacctacgc aatctcctaa ccttctttct aaaagtggat ttcagatgga aatagatttc
     2881 ttaaatgcaa gtgctccagc accaactagc aagaccaatt caagtctggg tatgctaacc
     2941 agccagccaa atattataca acgattagta ccagtagata gttttgatat ggccatttat
     3001 tccaacaatg gtaaagttct gtggaacaag acaaatgagg cagcaaccgg tggaagagga
     3061 actgagacgg tctcgctcaa cggcagctat actggtccta ttacaatttt gataaacaac
     3121 atcaaatcta gtaatgtaat gactgacgtc acaacaccat tatctactcc tactcctgct
     3181 aatactagta ctccttcgac cgccggtaaa ggaacaacga tagactcagt aagatttgct
     3241 tcaaagttga cttagcaatt ttcacatatt tccaattcta cgtgcagaag ctaggaagct
     3301 atctacacaa attaatatct cccttatttt ggcatcaggc tcatatgttg gaaggcggtt
     3361 cacagcaatt ataaatacaa aacaaataca tccatttctt cttagtctag gctaagtttg
     3421 agcactattg tgtcatctgt tgcattgact tctgcatcct tagccttcaa agtcttaaat
     3481 gctcctttga gccaacattc aaaaaaataa gaccattttt ttcccatctc atgtagaatt
     3541 acgtaagtgt ggatccgatc ttttacatca tgcctgtagt ggatacctga cgctgacatc
     3601 caattttcta cagcacttaa gtatgagatt atactgtatt ctccagttat catttcaacc
     3661 tgaccaacga actcatcctt caaatgctcg tcgacaattt gggttatctg ttcttgtgtc
     3721 agtttatcca ttattcgaac aaggtttttt ttcgaagctg acatcattcc agcttctgct
     3781 gcaggagaat gccagtatac atattctttg aagatttgat ttcctagtat gttaacactt
     3841 tccattttcc tttctgcttc tccacgcaat ttatctaaaa ccttcctttc aaccctaaaa
     3901 gtgatgctct cactttcact ctttcgtttc gcgtctctag ccatcggaga aatcatcagc
     3961 ctccatatta tggaatagaa catggtacgt aaaatagatg gctgcgaata acgtaattta
     4021 ggaagtttat gtaagttatt gcaatgtatt aatatgatta aatttattaa tttatttgca
     4081 attaattcct cattaatata taaaagatct ctcatcgcta tatgagagaa aattgatc
//
LOCUS       DAOR01000338           10263 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_23843, whole genome shotgun sequence.
ACCESSION   DAOR01000338 DAOR01000000
VERSION     DAOR01000338.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_23843"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    3030..3348
                     /estimated_length=319
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5626..5808
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2744 a         1786 c         1960 g         3271 t
ORIGIN      
        1 aaataaataa ctaaatatag acatggactt gtcgacaata gtatccagaa tatagaacga
       61 atgttgaact tgaaataact tagcataatt tgagatcaat ttcaacttct tgaattgtca
      121 aagcaaacgt tgttgatttt ttgtaatatt gggattgtgg gatccttatt ttttcagtat
      181 tatcggcaac agtaatacaa accccattta agagcatgtt cagtctcaat acgaaaataa
      241 tttgttacaa attgcagcta aaatcattca aacgaacgtc ttgctaattc ttcatattct
      301 aaccattgtt accagatgaa ccccaacatt caataaatag cccagcctac tgggcggcgt
      361 ggaatataca aagctttgtg aatcaatatt tgctttacac gacgatattc gctatgctgc
      421 tgttgtggat gatgcggggc tacaaattgc tggtggtatg cgtagaggta tcgattctat
      481 tgtagaccaa actaatgagg aattatatct gacacaaaca gcacttcgta aatccatgag
      541 acagaggttt gatgacacta tgggaaatgc aagattcgca tatgtagagc gcgaaaaaat
      601 ctcgatctta acattttacc taaataagaa tattctcatc gtaactatgg aatctaatat
      661 agactctcat agtgcgattg atatcgcaga agatacattg gaattgctgc gcgcaaaata
      721 aagaacgcag caatacaatt tgtatgtcta caccgatgat gaagatcact gtcttctttc
      781 cggcatattg aggcagttgt gtggcgtaac attgcaggga gattccagaa acctgctgct
      841 acgctatctt agcttttcaa attatgcatt gtctttagat cttccttgaa taaatctaaa
      901 tcagggggga tgatgatgct accaatagaa tccttcagag ataacggctt tccggtttcg
      961 tctgaaatag ttgctttttt cttattccat accgtcgagt taaaatccat aatcaattta
     1021 gtatattttg tgagttcacc ataatgatcc agtggttgag gcatttgttg aagatgaata
     1081 ctctcggccg agtaaatttt tgtccataat tcctcagtaa taaatggaca tatcggggct
     1141 aggagaacta aaattgtcga gaaacatttc tgggaggtaa atatggccga tctttgccca
     1201 gattgatcac ctgtatcgta aatgcgtcct ttaatcattt ctatatagtg tgcggcaaac
     1261 acattccatg taaactctct tatcccattt gcgggtatga agaaattaaa attagaataa
     1321 ccgtctttgc atttatcgat cagttgggat aattcaccga taatccatcg gtcagatgct
     1381 aagagtgtgg caggcgtctc taaaataaca tcgaaagaag atagaaatct gccaatattc
     1441 catatttttg ataagaaatt ctttgcagtt gtgattcttt gctcggagca acgaaaatca
     1501 taacctaaat tactttctgc tgctgcccaa aatcggaaaa catccgcccc gtatttacgt
     1561 ataaggggaa gaggatcaat tacattccct ttacttttgc tcatcttttc gcctttctcg
     1621 tctagaccat atcccataat ccatgcgtca gaccatggta atctactggt aagctggaag
     1681 catcgcaaca ttgtataata aagccaggtt cttatgatat cctttccttg gggtcttatt
     1741 gtagtcggat aagtataatt gtagaattgc gaattattct tatattttgt aataaacagt
     1801 ggagtgatac tagagtccag ccatgtatca aatgttctat cttctccgat aaattcagtg
     1861 ccaccacaac tgctacattt ttcaaatgga ggtttttctt tccacggccg ataatatcct
     1921 ccttcatctg gcagattagg atatttgcag ttattacaat accatattgg cacttcggta
     1981 ccataaaacc gtcttcttga gaccggccag tcaatcgcaa tagagttgag ccaatcaagc
     2041 aaaatttgtt tatgcctatc aggatggaat gtaattttat ttgcaagttc ttttaataca
     2101 ggtacataat tgatttgctt tacgtagtaa tcctcaagcg atatgatttc gactggagtc
     2161 ctgctacgtt cgcatattgg cgtcctgtgc ataatatttt cttctttttc aagaataccg
     2221 atgtttctta attcttcaat gatcttggtt ctcgcttgat tgaccgaaag gttagcatag
     2281 accccagctg cagaggtggt tctcccgttc tcatctaacg atacaatttc ttctaatcct
     2341 agctcgcgga atatccgaac atcattttca tccccgtagc tgcaaaccat aactacccct
     2401 gaaccaaatt ccggttttgc aaaagggtga gccataatct tgacctctct gttgaatatg
     2461 ggtaccagag catgcttatc tatcagattt acataccgct cgtcttgtgg atttacaata
     2521 attgattggc atgcaaaaag tagttctggt ctagttgatg caaccacaat actattcccg
     2581 gtttcgatta tcctgaatct tatgtaaatt agctttgtcg gaatagtctc gtagataatt
     2641 tctgcatctg caatagttgt tgcacactga gggcaataat tatttggcct atttgcacga
     2701 tatattagat tctgtttcca taaataaatg aaagtactct gtgttaaagc ccgaaattcc
     2761 tttgaatcgg tacgataata ttcttcaaag ttgccgctaa tgcccagagt tttcattatc
     2821 tgaatcatct catcctctag atcgtcaaga gcatgagcac agagttcaag aaatgtttct
     2881 cgatcggtct tccgcatcac gattttatat tttttttctg tgtaaatctc aaccggtaga
     2941 ccgtttctat caatgcctat cgggaataac acattgtacc ccatcattct ggatgttctt
     3001 gcgatcatat caatttgtga atagtgagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ctatagaaaa
     3361 cttgtagatc gcattattag cccatttgtg cagaataatg ttctccaagg caggattcca
     3421 taacttggct tcaattctgg gcaccatttc acactttcat ctaatagttt atccagtggc
     3481 gtatccatac ggttattaat gttagtatag ttttggaatt taataacaat ggccgataga
     3541 ttattggtgt tgcatatatt gctaatcatg atagtaataa tgtttttcct agtcacactt
     3601 ggtagttcta ttttttttac aatgacaatt ttttcttcaa gagtatccgg agaactagca
     3661 ggaaatttaa tggtgcttgc actggtaccg ccttcaattc taacagtact atttctgcat
     3721 aaagttgctg gcaagttata tgaccgatta cacgttgtaa taatcaaatc ttcattaaag
     3781 aaataacttc gtaaacagat tctactcgga gaaatcgatc atctagaggt actatgcgtt
     3841 gcgtgtagcc tatggtgata tttttttgaa ttcctaaaaa agacggggtc tttcattccc
     3901 aatgacctga aagcatcgag tcgttgtttg catgcgtaac attctccgca atgccttctg
     3961 gcttttgtat gacaactcca agttacttca agtggtactt tcagatctat tgctaagttg
     4021 attatatgct ttctatccat cttgcttaat ggggtcacta tcacacacgg atcatgattg
     4081 gaaagcaaac agccgtgctt gaacagtgaa tttatttttt ctatgtagtt cttcgtggcg
     4141 tccttaaaaa aggatccatc atcgctgttg tggcctccga caatccattt aatattaaga
     4201 aattctgcat agtgtgcagc aatggaataa aagatcagat ttctagcggg gacatatgat
     4261 ggccatctgc tatccgattt ctgacggttg ccaatctgta aatctgaata ttctttaata
     4321 aatggaatat caatctccaa gaaattagaa atgcttgctc ttttcgctaa ttgggctgcg
     4381 gattcttttt ctttttgaat tctgtcgtaa caattaaaag taatggctga gacatctgaa
     4441 aatttttctt ttgcccaata aagacaggta gcagagtcta gcccacctga gagcatcacc
     4501 agtgcctttg atcctctcat tacctaatcg ttttttactt cagcaattat atgggcgccc
     4561 ttattgactc cctcgtaaat atagacgccc aatgcttcca cgttgggggg catttttggg
     4621 gctagcaact ttattatttc cgttgaaaga gtttctaccg ttgcttcagc tggtaataga
     4681 aatgttgtta gttttggcaa ttgtagattg aagtatccac gaggtccatc aaaagaaata
     4741 aaataataaa gatcgtcttc tttttgcagg tattttttat taatgaagaa tttatggtca
     4801 ataacattta aggtatcttt aattatcttc ttggcttcac taaagtcgat cacaaggtta
     4861 tttgtcattt ggccaataat ctctaccatc acagtggaag tatgcccatg caagatagaa
     4921 catttctcaa ccagtgggag aatatgcgcg tagtcgaatg caagtgaagg atcctctata
     4981 aatatagatc cgatgtgctt gctgtggtta gaattataga aaaataatga gtctaattca
     5041 ttcaccttcg ctgcatactt tgaatgacca agggcaatga catcgacatc agggagttcc
     5101 ttttttatca gcttgaattt atccatgtct gcttttgaat caactatttc gatgtaccta
     5161 ttacctgttt tgaaatcgat cttcgcgctc gtaccatcct caaagcttaa attagcatca
     5221 tactcataaa ttattcctag atacgacaac agctttgaaa tggagaactc cgccctgctt
     5281 cgtaataggt tcccattatt atcaaaatat ctgatgtctt tatcaaggac aactctttcc
     5341 attggaatat taaaaaaagg attgtcacat ataaattcac cctcatgtgc gatatttttt
     5401 gatatttcat tttgttgttc tgctggtttt gcaggtaatt tacgtacgtc atgaacaggg
     5461 caggaagcta tcattcatgg cgcttagcac tgaatcgtgt ttggactggc catgggttcg
     5521 tcaaagtatg ttaaaccatt tatctcagat gatttttgtc gacattccgg ggtgataaaa
     5581 ttgtagtttt cagtcatttg atacagagcc tagtatcatc ataccnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ctagtatcat
     5821 cataccaggc gcagcagctt tctaattatt aattctgatc attttggttg tcaaaatcaa
     5881 gctgattttt ggaccaccac aataagcatg aaatacttgt gacttatttt gctgcaggaa
     5941 tgaacacggt gcttttctgt ggaatagaag tggcggtaga cgacactgaa acccctgaag
     6001 ctggaccaga catacccgag tcaaacccga taacacgcat tctatccaca aggtccaaag
     6061 ccatagtact agatagttga tttgaagcag gcatggacgt gccgttggtg atatggagca
     6121 aaggtgtagc tattacaaag ggtattttat cattattatt tggtagcagg ggtttttgaa
     6181 tagggctaat accttgattg acaggactct tggtactatt tgtctgagga tttgggacgt
     6241 taaccttgtt ggaaatggcc gggatattga tattgtggcc agggttcggt attgaaactc
     6301 tgccgctctc aaaacttctg aaactattag caattggcgg aacagtggga ttgctaagag
     6361 ttgtgctagt agtactaggt aggtaaactc tgccgctttg attacttaca ctactctgca
     6421 aagcaagatg agttgagttt tgttttgaag agataagtgg agagttgtga ttattattgc
     6481 tttttgttgt tgatgatgtt gccactatta ccgatggtgt tactgttggt ggtggtgaag
     6541 ctatttgttt cgaggtggtg acattattat tattattgat attacgtagg tttgatgaag
     6601 cactagagct attattgagg ctggcgattg gaacagggac tactgtgaat gtttttgaaa
     6661 ctgaattatt ttgataacca ggcgcagatg tctccattac caccttatac ttgccacttg
     6721 tgttatctcc actaacttgc catgaatatg agacttttcc cttctcattt gtaatacccg
     6781 ctaacttttt agatatccca gacggaactg tgatgcttcc aaatactgat gctccagcaa
     6841 cgacatgagt acggttacta tcagtaaccc caagtgttag attttcttta tctccaacat
     6901 gaacggggcg cttcgctata tgtatcgaaa ccgataatga cctggcgtta ttattgtcgg
     6961 ctttggtgtt agttgatgtg ttgttttgtt gcttcccaga ttcagctgaa cctttgttat
     7021 tcaggctatt atcattagtt ttggttgttg tagttgatgt cggtcctgat gaagatttcg
     7081 aagttgtacc tttctgatga ccgtgatcaa tatttggatt tgtggtcccg ctagtttgtg
     7141 cgtcaccttg atgtgtggat aagatgcctg cggtagaaag cagtgcggca gcactgacac
     7201 ttgtcatttg tgatggctgg ttaacctgtt gcggtccctt tcctatttgt ccggtgacgt
     7261 ttgcgttatt attattatta ttatttgctc cgtttatcgg tatcgcgttt gtttttgttg
     7321 cgtttgtttt tgtcattttg ctattgagtt gttgagaatt cgtagggata gaacctgatg
     7381 tctttgtagt attattagag attggtgaat gcttcttctc gatactactt tgagttttag
     7441 tggcgttgag actattattg cctaccttgg tattacttga atttttaagg gattgtcctt
     7501 gctgtttcgt cgtagatgtc ggcgctcctg taacatttat gctatagaat tttgttaaaa
     7561 gggttgaact gttactagta ttggcattat tttcttggca ggctagtttt gatgttatct
     7621 tattcgttcc tgttttgatt gtagtgtagg ttggaacaag attaaagacc catgttgaat
     7681 agtcatttgt gttattatgg ccgccggtag gaagtgctct ctggtatggt ttcatgtcat
     7741 tcacaatgac atatacttta caagccagcg tgccattgtg agatgcaatc ccgctaatct
     7801 tcagattatt gccagtgagt gagactggtt ggccctttgc aggagatgta attttaatcc
     7861 cgacagtttt tgtcggttgc tgcgccgcag cagaattcac gatgaccgtt gaaagaatga
     7921 ggagcgtagt aaatccaagc aatggtatgg atttattgat tagcaacaat tgccacctcc
     7981 gaacacaaca atgtattgat cataatcaca gctcatttcg atgggattag agtttgaaag
     8041 tgcagtattg atagatttta ctttagaagg atccatttgg catctgacat tgaagatagc
     8101 cagcagcgaa gtactaattg tcggataaac cattacaatc taaccaccac ctttacatat
     8161 tatatgaact tcgtctaagg ttctaataag aaaaattcat ttaattctcc ttctgatctc
     8221 ttacgatcgt attatacgat taaacggtac aattcaattt agttaaattt agtttagaat
     8281 gcatttaccg tgaaatttta cgattagcat aacaactata agtgtttttt ccttttatat
     8341 tcgcagctac gttatgccac cgttagagga aaagtcatcg taaggtacga cctgtcatcg
     8401 ttcgatttta ttacgcgctt gaaacaattg gaacattgca taactcttgg tttcaaggcc
     8461 tatctcacaa cttttactga caataacatt tgtatggacg gtcagcagtc taaacatacg
     8521 gacgcctcta atagtaactg acttttctat gatagatgta tacgataaat ctttgatagt
     8581 atacacaaag atagaattct gaaatgcaag aataggtacg cacacctaac gcagtggaga
     8641 tgcaagaaag agaagaggaa gcgaattgac tgtcattagt attatttcaa gtgtatacgg
     8701 taattgtttc aatcaatgta ttggatgtga aaatagaaaa tctacgacat ttatttgatc
     8761 tattaataga agctaacaaa aagtccttgc gtaaacggaa ggattcgtat ctgcaaatat
     8821 tcacaaaggt ctggatggtt attatagtca cattggaaca gtgataggca agtcctaatt
     8881 gtggtaaata cggttttgta caaaaaaatt atggatgtgg caggggggcg ggtcgtgcag
     8941 gaggatgagg aattggcgaa ggaggaattg atggtgcagt gagaaacgga atgagtgatg
     9001 gtctagatgt cagtgttgct ttcaggtcta tcgcgtgtcc atttctatat acagttaagg
     9061 taatattgtc accaacagac ttatgttggt cgatgtaaga gatcaaatcg tcaattctta
     9121 caatattatg tccatcaaca cctataatca catcacctgc gtgtttcttt gagtattggt
     9181 ctatagtact tccctgtaca cctgccttat cggcaggtcc attcttcgtt attgtatcta
     9241 tatatatgcc cttaagattc gttgctgccg ctccgcctgt aagattttgt gataaatctg
     9301 atgtcaaggt agcaccatta agccctagat aaggatgtaa ataataccct ttttcaatta
     9361 atgctggggc tattctgatt attgtgttag atggaattgc aaatccaatc ccagagaacg
     9421 ttccagttgc agaaaatatg gcagtattca ttccaataac ttgtccttgt gtattaagta
     9481 gaggtccacc agagttgcca ggatttattg gtgcgtcagt ctggattgta tttggtatgg
     9541 aaaacccaat tcctgaagca gctggaagta atcggcctac cccacttaca atccctgttg
     9601 tcatagtatc actaagccca aatggattac caatagcaat tacctgatct ccaacttcga
     9661 gtttagaaga attgctaaga gcaagtggtt taacagaaga taatagctgt tgtgcttgag
     9721 tattattctg cgaaatttgt agaactgcta tatcactata gatatcggtt cctattacct
     9781 tcgccgtata tctatttcca tttacaaatg ttacatctac tatctttgca tcacctacta
     9841 catgaccgtt agtgattata tgcccctggt tatcgtatac aaagccagaa cctaatgtag
     9901 tagcatttga ttgaggattt agagggtcag ctgttgctgc tggtatcttg ctagttattt
     9961 gcactacaga tgcctctgct tgcttgaaaa tggtcttgag aggtaaggag gtagcggaag
    10021 aggaggagtt agtattttgt tgggttgagg ctgaagaatt tattggatct tgttgtggtc
    10081 gttgttgtgc cgccataacg gtaagtggaa tcgtaaacgt actgactgaa attattatta
    10141 ctgttattgc aaatacagtg actataatag ctattgtagg tacaactggt tttctcattg
    10201 caatcaccta tgtatatgct cttataaggg ttattcatcg atttagcttg tagcacaata
    10261 gat
//
LOCUS       DAOR01000339            3468 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_67545, whole genome shotgun sequence.
ACCESSION   DAOR01000339 DAOR01000000
VERSION     DAOR01000339.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_67545"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         1046 a          669 c          644 g         1109 t
ORIGIN      
        1 agggtttgtc agtctcttca ctatgctaca ttcgttcatt tatttcaaat ccctgatatt
       61 cttgtaattt tcgacgactc gaatattttc gttaaaatct gctgcatagt tagaattttg
      121 tccatatggt gttcccttga acgactcgaa gtcctttgca tattggtctc ccgggggtgg
      181 ctgtgtaccg tattgatatt ttccacgtaa ttcttcttcg tctatcaaaa gccacaatgt
      241 cttaccgtca aaagcatccg caacatattt tggaacccag aatttctctt tgcggacagt
      301 tcctttttct acgtgtaagt agttttcaga tacctcttta atttccccta ggtcttttcc
      361 atcattagtt tttacattct tgcccttaag cgttctccac aatgtggtag gtcctgcttt
      421 attactatca gctgtcatag caatatgcta ctatattagt gaaaccatgt attttaatga
      481 ttaaggtctg ttgagcttgc tgtttttaca tctgttctac ggtcaaaaaa tataataact
      541 gataaattgc atgatgtgaa actacaatca ggattaaaga tgaccgatat tatatcatgg
      601 gataaagcca ttgatagtaa agtaaagtaa agtgatgata aagatctagg caagattcaa
      661 agtgtaacga gggattatat tcaaacaaaa gaaggaacgg taggtaagaa gtactattat
      721 atcccaaaat attatttcca aggatacgac ggtgatcatc tctgggttca ttcacaaaag
      781 atgacgttaa atcaaaattt gaaggggaaa aagcaccgcc tgaagtaacc tttgaagccc
      841 ctggtttggt agatacatgt tgcaaaatga tataacgttt agaaatagct agcattgata
      901 acaaaaactg tcctccactc agcacaggtc aatttgtacg ttctcagatt gttttaacat
      961 aaaatgaata ttaaaccgaa ttttattcat acaacaagtg gatcactaac aacagcctac
     1021 tacccgagag atcatggggc tgagttcaat ggtggcgatg gcgtcatttt agtaggcgca
     1081 gatgatggat gatctaagta taaaaaattt ggcatctaaa cgaatgataa ccaatagcgt
     1141 ggaaggaatg cagaataaga ttaattaccg aatttcttct aggatgctat taataattct
     1201 atagtcccca atgttttaga ttatcaagaa attcttgacc ataacgacgt gcaatttctt
     1261 gttctaactc ccatgtaatc tgaccagtct caagagtttc agcaacaacc ttaaagtatt
     1321 cttctagtcc cgcaggtgag ataactagta ctcttccacc tttggagcct gactggtaat
     1381 tgtgtggaat accttttgga atgaatacga aatctccagt tttggcgtga taagatcgtt
     1441 catcataatg tattaaatac tctccttcaa gtacataata cgcttcgggc gcagctggat
     1501 gcatgtgaag agcaggacca atatttgggg gatgaatcat ctctattaat gaatatcttc
     1561 cttcagaatc atcccccgaa actttaatga gcatttttgt gccacgaaag tttatttctt
     1621 caccctctcc ccgtcttatt gcaaaaccgt acatcaagta ataaatcaat actagtataa
     1681 tatattcata aaggtgatca tctaggcaat tcaatacata tcaaacgtat ccttctcagt
     1741 tccatttcaa tgaaagaaga aacgaggtca ttctagcata acagatgaag aagtctacgt
     1801 ccttctccgt aaaattcctc ctggcaaagt ttctacttat ggagatatcg caagagcgcc
     1861 gggacacccg agtcaatgat ataaacatca tttggattac aattggtata tacctcagta
     1921 gatttctttc tctacaaggc cgctgctaat aattaataca tatttacgtc aagtacacaa
     1981 agtgacaaaa tactaatgaa aactcttttt atacttgcta ttgattaaac gttttatggt
     2041 cacaacccag catataattc aaaattgtta tatcacggta tagcttttat gaggttctct
     2101 aaatttctga ctgaggtgat gataaacatg gcttttgatg gcattacttc cattcctctg
     2161 acctggggga attcgcacca ttgggggcaa ttaccgaagc gcattttgac aaaacattca
     2221 gcgtcaacat gaaagggcct actgttcacg gtccaaaaaa gcggggagga tgctaaagaa
     2281 ttggaatctg tccctgaatt ttccattctt ggagtaggtg cccccataaa atttgcagat
     2341 ataaacgatc gcatagtaac gcttgtaata cattcgtcga atgtatcaaa tagtccagac
     2401 tcgaacccag agacaactcg cttaaagaga aatgcacggg agaacgtatt agaaaatatt
     2461 atatctatta tcaagctata gctattgtaa tattggcaaa ctgtcccaga tgcggtaaaa
     2521 gaatagtaaa gtggtttgag acctggtatg ctcaagggaa ttgtagctgt ctatctgtag
     2581 aaaacaaaac catagggaca cgagatgaag agtattaatt ggatatataa taaattttga
     2641 ctggttcttt attattgaat aacagaatgc cattctcgct catgtttttt tcgctcgtca
     2701 atactacgat attgtaattt gcaataatgg catttcaata gtggttttct ttttaattcc
     2761 ctcagtgatt gagatcccaa catcacattg gataccttta gcctgcaata cctcctccgt
     2821 taggtgaaca atctcctctt tatgctattc ttcctccttg gttagcacgc tattacttct
     2881 aaatctaaag aaggcaaatt cactcagagg aataggtatg ttattaataa ctcgctatct
     2941 actttacagt ttaccgtata taaacaacct aactattgtt ttgtttggtt ttgttgcagt
     3001 gcctggtgct gcgtaggtgg aagtggttgc tgctgcgtcg gcagttttgg tagttggaaa
     3061 aaaggaaatc caccaaaact gccactacca ttactaccgc cgctactacc aagagcatat
     3121 ggcatttggc cattccacct gttgacagct aaccactgaa gatactgtgg actttgtttt
     3181 aattgtgaag tgatgacctg gattgcctga gattctccgc tagctctggc tacattcgcc
     3241 ctagctgctg cttgagcttg tacaactgtt tggtttgcta ttacctgtat agctagtaga
     3301 ttattttgtt ctgttagaaa cttttggaaa gctactactt ttgattctac ctgagtagca
     3361 aatgcttgtg agaatttaaa gtcagttatg aatactgtct cgacaactat attccttgct
     3421 gatagtgtat tggcaatact cccggctatc acagcttttg cagtagct
//
LOCUS       DAOR01000340            9297 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_63728, whole genome shotgun sequence.
ACCESSION   DAOR01000340 DAOR01000000
VERSION     DAOR01000340.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_63728"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
     assembly_gap    5670..5817
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3062 a         1682 c         1753 g         2652 t
ORIGIN      
        1 cattagttgt aggtaatact gcaatatact gcaagctagt acacgtccag aatttattat
       61 gtgtcctccg tatattgtgt tgacaattag attaggtttg ctatcagaag ttaggtttat
      121 accaacagtt acgtcataat atgtaccatt gggaagaagc ctaacacacg taccgtttgc
      181 ataaggtatg tcgcatgcac tatttgaata agtgggagct agaaagtttt tacttccaat
      241 accgttcata tgaattgggg ttacaccaat accactaaat ggcgtttcag catgttctct
      301 tgctatgttg gcgatccaag acgttacatt gcttgtacgt atttggatta aaactgcatt
      361 tccttgcgca tatgcctcaa atgttataga cgccaccata cagataacac ctattcccgt
      421 tgctattatg tagaaaatga gtctcaaaga gattgttttc attgtatgtc ccctcctaat
      481 acagcatgcc attcaaaatc atagccatag ttgaaacctt gacagtaacc agcggacaat
      541 ttagatccgt taattccatg atcctcacaa tgaccacgat agttagtacc gttgttaaag
      601 tcattgtatg catccaaagt ccctaatgat gatgcagaga atgaccaaac aacaaatcct
      661 tagatatcag atcatatcta cgaagagata ctttggtaat aacgaataca ttgaaagcga
      721 tcctaatcgt gccagcaata gccgtactat taagcgccgt agtaccgcat acacaaattg
      781 cattcgcaca agtaatgaag ttgaagcaac atccagataa tccgccaata ggcaacatcc
      841 agccacacac gcctccacaa ataagtaata atcctcttgg cttcgtggcc cctggactcg
      901 gctgatggac actttaatgc aaaatacgta caaggatact tcgtccaacc atgtgatgat
      961 cttgtagctt tatttgaaag taacaaagat agagctttat taatcataat aacaaataac
     1021 caactagtat gatttggccc tgttagcatc aacataggaa tagctatttc gataaccagc
     1081 aatttagtct gcatcttttt ttctttactc ttgtttgctc taactcttgt cacttacaga
     1141 aagcacgatt ttccatcttc atgagcaact ttttatctat ccctatcttc taatgcattg
     1201 attcgttctt ctaaatgtgc cacataagcg gctgcattac caatgtctcg gaaatgttct
     1261 cgttttagat ggtcttcaag tttgccacta taatccttat agcaaaatcc acatgttgtc
     1321 attccttttt atcttccaat cctctattaa ttgagtttaa gtatctcact atgtttaaac
     1381 cacagtaccc agtagtattt actaacccct ttcagtgcgc tacattatta gtataagtgg
     1441 tcgatatgcc atttcgatta cctatattat tagtaatatc gttagggtag caattaagaa
     1501 aagagtagcg ttgtgggttt gctacaaatg ttatcggggt aagaaaacca ttaaattcaa
     1561 ggcttagtaa tactgtatgg ctcaaatgga ctgccctatc tgtacttcag ctaagggctt
     1621 gaaggataga caagaacttg ttcaacatat ggagacccac agagataatc cagatttgat
     1681 gatggaaaaa attgccgatt atatggctgc tcatgaaacc tcacgagagc tgcaatgaat
     1741 tcacatgcct aagaataatt agtatgcaaa ttggctatga gcgtgcaagc ctagccaatt
     1801 tgaaagtcgt gattatccta cacatcttgg cctatgcgtg gtgtgaaggg caatgcaata
     1861 tatcgcaaaa gtagcaaata aatcttattc cgtaccgcaa gcagctgcaa ctgctgattg
     1921 aaatatacga ttaggtgtaa atagaagaaa atctcattat gctatgacaa ttaggagaag
     1981 caggaaggct aagaaatgac tactataaat ttagaaaaac taagtaaaca actaaaccat
     2041 caaaagctag caataattga gttggctagt aaaggggaaa tcatatttga acatgcgcat
     2101 aaaatgataa ttcagcttaa cgaacttcag aaggtttatc aacaaataat tgagacgcaa
     2161 aaaagaccta ttttcattcc acatgagaag gataccacac tcagtagaga aaacattaat
     2221 catttattgg tgacattgag aggctacagt tcagaaggtc aatccatttg accaacccca
     2281 aaatcagaca gcaggtatta gaacgtgaca tggcatctac acaaaagaaa aatatggcag
     2341 gatcattaaa ttactctcat gacgactgag ccaatccttt cacttgaatg tcaatgaggg
     2401 tatcttagag aaagatgctt aagtcaggaa aagtgttctt gtaaatgtca tgatcctctt
     2461 agaccatctg tcgatttaga tgaggaggca agacgaaggg aagaaagacc tgaggaatac
     2521 gaaactaagg taacataaag aagaagaatt attaaccctt atttgtagac agttttcaaa
     2581 ttattaacca tccatgagtg ctataactgt aattatagat tatcggttct attcttactc
     2641 atttaacaat tgcttaactg caccttcaag aaagccgttt gaaccaaata tggtctgtcg
     2701 taatacaccc ttcttgtcaa ttaggatagc tgatggtgtg ccacgtaagc catactcctc
     2761 aaaagtcttt gctgaatatt gctttagttc taaatactgt tttgctcgct cgtacattga
     2821 ctgcttgtcc tcctcgatat aagatctgaa atctgggata ttagcttcaa tgaaatccat
     2881 gaccttgtca tcagtcaatg gaccaagctt cagcaatgta tccattccta ctgggaatgg
     2941 aattttgtaa cctagtttat ttcctggagc caagcggcca tactctccta agacccttct
     3001 tggtactcca acaacctcac cagtggttag cagcagcttg aggttttcta aagtattctt
     3061 gtcaaagtct tcaaaagccg ttgccactcc taatactctt agaccatcat gatgatattt
     3121 catatgcaag tctattgcct gtgggatccc ttgtgtgaaa caacctggac aattaacttg
     3181 gaatacttca acaaggacaa catttcccct ttctttatct atgttggtag ggtttccttg
     3241 tatccattca gaaacagcca gattgggagc tttatctcct atttgtggag gcaatacact
     3301 acacatattt tcagcaacat ctaatttaaa cgcatagacg ctaaatatac aataattggt
     3361 cgttccattt ttggaatctt gcaaaattaa tcataatggc ggtcatatat caacagcagc
     3421 agtacatatc gtagactagc tttgctattg tgactttgcg acctctgcca cttattgcaa
     3481 gatcacgacc attgttgttg agtccgatga gatatgaatt gcctttgccc actaagattc
     3541 attatacact ttttctttaa tcaattagca tcagatttga ttaatcataa agaaaccaca
     3601 ccagagtatt tatgatttgg attttacact taggctatga aaagaaaaac attcgcaata
     3661 ttgggtgtcc tcattggggg cgggttagga atagcagtcg agtacaggat tagaagtaga
     3721 agtagcacag ggagtcatgt tgagctagag agatgcaaac atggcaagaa aaatcctggt
     3781 atgatgattg ggtgatatat taatgataaa aacacaatta attttagtag cagcgccatt
     3841 attagtgctc atatgcgcac cactcgcata cgcggacgca tcatatgacg tgggatacaa
     3901 tgcagcgaaa ttcgacttcc tacaccataa atcatataac gactcctgtc acactgattg
     3961 tactcagtac agagtgggat actctgaagc atggaatacg gtaaatggct ggttaggaaa
     4021 tcgttaagcg ggcttcctga gaaagaaacc ccgattggcg cctaatgctc gtcgaagtct
     4081 tgaaggcttt atgatcggtg gaatacgaga aatgcaggag atgcttaatt tctgccataa
     4141 gaatagcatc gatactcatt tgaaggagga attaagtaag catcgtatct cctctgaaga
     4201 ccctgataag cttttagcag tacttaataa catcaaaaca ttatcgatac gattctaaga
     4261 agatagtggc ggattctcta acatcaaatc gctaaaacta agagagaaag cattgcagga
     4321 taattgcgaa atgctcgaaa aacgaattca gtgaatgagg attcgcgtag acaaattact
     4381 agtatttagt gttgcagtaa acgagaaagc ggagacatat aacatgtcaa tttccgctgc
     4441 tgcttatcgt gtgataatga tcgacgacat agagaattat aatagaatcg gcggactaaa
     4501 aaaggaaata tccagacttg ctctccaaat atatagaatg aacaaaattt gcgcgataag
     4561 aaaacaaagc gatcacgatg ctgcttaagc tgcaggcatt cggtaacatt agcgttcaaa
     4621 tcaaatagat gaaggccgcc acaaccccat ttgataaacg aaaatcaacc ctggaggtaa
     4681 tttgtcatta gttaactagt cgcaaccttt gaatacaaat agaacaaaaa taataataag
     4741 agtcgtggtc gcgccagaag ttgtattgga acttcaaagc gtcttccata tctgttaatg
     4801 atcgtatgac tttttgcaat agcagcttgt aaggaacatc tgcttggaca gtagcctcaa
     4861 atgagtttgc accagatgct actttctata catttcaaag tggagtagat gacaatttaa
     4921 tgatgggcag agtagctaaa cccatccaat acattggaga ttttggtcaa gaaaagcgca
     4981 taagggaata atgttataat caagaagccc aatcatgatc atgtttagaa agatggttga
     5041 aacatgtatc tattcttccg agttggaaaa gctgaagaat ttttacgtca ataatattgg
     5101 attacaattc gtctctgaag aacctggtag acatgttttc ttaaaggctg gaaagagtat
     5161 gttgctgata tttaatcctg acagcacaac caataaaaac caaattatgt ttccacccca
     5221 cggtgcatca actccgccag caagcgttca ttttgcacta gaaatcgaaa aagaagatta
     5281 cgaaaatgca aaaaacatac tgatcctgaa taaaatcgaa attgaaaaag aactaacgtg
     5341 ggaaaatgaa aaattaaaat ccatttactt tagagatcca agccgtaacc tcgtagaatt
     5401 tattacaaag ggtaattggc cggcagaaga ttagttgata atctgatggg aataaccctg
     5461 gcctttgact aggcagttat gatcagtgga tctccattgt tgccgtgtag gttaaaggaa
     5521 atgaaggctg tgaaggccat agagggtaca gggcatgaat ttgttatttg tgattattag
     5581 atcagaaaat atgaactatg taatcgttta agatgacttt gaattcaaat ttttgcttct
     5641 aaaagtacca aaagtctgcc aataaaagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg
     5821 attgtctatc ggttgtaaac cgattttatc tagttcattt ataaagcgtt cgttcataaa
     5881 ctttgttttt agcttgaacc atcgtgtttc tgtctcttga ttagaccttt tatatcaatg
     5941 cctatcattt ctattttctc atgccacata actaattctc gctctgagtt ggtatctcat
     6001 tacactacaa tgtctaatga gatctgatct tctgctggta taatcaactt ttgaatccaa
     6061 tccgaacaat gttttctgca ctaatcaaga ccatcctttc attctaccaa ggcatgtttc
     6121 ttgtaactct tgtacctccc taattctgca taggataaac gatttctaca ttactcgaac
     6181 cacattagtg gctaggtaga cctaaataat aaaaaaagta actgtatctt attatagaat
     6241 gtttcacgta tatctccatg aaagggaaga aggatataca aaaagagaca ttgaaaccaa
     6301 tcactaaagt taaatcagag gaagataagg aattgcaaga gttagaagta gaatcaactg
     6361 gcggaggagc gggtgccgga ggaggctaag ctaacgtcct attctagagt gttagcagcc
     6421 attcctattt aataacaaaa ttttttgaat cttttagacc attaggcttc tcctgaaatt
     6481 tgctctaaga aggctaacct tggataagtc accagatatt gaatatcgcg aggttacaac
     6541 atgggttgat atcaatagta gccacgagta tgcaaatgtt tcaagatgtt tccttgtcag
     6601 gtctctatct tttaataatt gtgcatttag attatttatt ggtatattgt gaaccgtcaa
     6661 gcaatgtgtc ctatttgtaa acagaaattc atcgaacgtt cagagctaca atacaaaata
     6721 tgcaaaatga tcacaataaa gcaattgccc cattaaacag tgaattcaag acacaatctg
     6781 aaagatagaa gatattgatc ccaatatcac tgatgaaacg gagcaattga ccacaatagt
     6841 agcgataaaa tgtcctgacg caattgtgat ggctagtgat agtcaggcaa cagtaagggt
     6901 cgaaaagatg aagactctgg gtgtaaccaa aatattcaat ataaataatt tcatgggcgt
     6961 aggcggttaa ggggacgcag ataacataaa gctgtttgtt gaaaatttga ttggagaatt
     7021 ccctcagatg ttgcccgcgg aaagcgaatt cagggataaa attcaaactt gctgctctaa
     7081 gaagaatttc gctgattgtg tctgcgtcct cttgtttcga gctcaacaag aaggcgattg
     7141 caaaattctt gtatataaac taactcgatg cagatgccag aaaacacaga tagaggtata
     7201 ctgctgacgg gaagccagtc tatgagtaac tattccttta aatgactaac tttgtaacgc
     7261 atgtatttgg tctgaattct gatgtaactc cacgtattgc attacaacca aatgtttatt
     7321 ggcatcgatt ttagtttacc attcatacat gaaggacata tttcaaccgt gccgagtaag
     7381 ctaatggatg atgcacacca ataacatgaa ccgcatagta agaaatgtgg ttgctcgaaa
     7441 gtcgatttta tcacactcct ttcttcagtg tgtgccacta ggcctggtgt ttgtgtaaac
     7501 aatagttgaa acatacctat aatatgcata tatcactata aatagatatc tatctttgat
     7561 tatttcaaga taaatatata tatgcacaca aagtaaacaa attagcgcag attcctgtaa
     7621 aaaaaagttt tattcattgc atcaagattt cctttatcca tagtaatttg aaggctacta
     7681 tgtctaaatg taggcaatac acaatattgt aaactggatc ttacgaaaca tttcccggaa
     7741 gaggaacaaa aggttcaggc gtagaataag aaaaggatat aattaaactt gagatgttaa
     7801 atcttggaag accttatgag gtttggaaaa aagtgctatt aaccgctata gattacctgt
     7861 accgaggaat ttgttagaaa gagtagacag aacctcctcc ccagctcacg caggaacctt
     7921 cgaaacgcaa tagatttcat actcccagaa aatactcgct gcagccaatg gtatagtcac
     7981 ctatcttaaa gacgattcta tcattggaac tttacaaatt ttgtagctat catgcatgcg
     8041 aatgaagaat attcaaggta cgatcacttg gcacctcaaa gctcaaaagt caaagtaggt
     8101 caagctgtca gcgaagggca agagatagcc agggtcggga tgacatgata tacatttcgt
     8161 ccacatctac acaataggtt ttcattttta ctagagctaa tatctggata gattttggcc
     8221 tacggtaagt gttcaagatt ttagctttta gtaagctaat aagtactaag attgagggcg
     8281 ccatcaatat caaatctaaa ataggatatg agatagtgac aacaactgcc aaaaaagtca
     8341 ccttcaacag aatgttgtga agaggagaat agacttatcg aattgtaact aacgaacaag
     8401 actggaacta ttgatgccag gatcaccgtg atcaaattgt tcttagtgcg tatgtagctg
     8461 agtactgtaa acaaatgcga tgcaagaagt aaatatccag caaaccaaaa aagatacgta
     8521 acagatacaa gtgtttgttc ttctatacct aaaacaaaat agtaatatgc tatactcagg
     8581 tctgcagcga accaagaaat aagctcgcac ttttctacga gtggaaacta aagtaagatt
     8641 cacgccatgg tacgcgcatt attttcggtt gactcaacaa agtattcaaa gtaacgttag
     8701 tcaggtgata tgtattatat agcttgtggt tggtataaaa gaagacaaag agattgagac
     8761 gagttaggtt aacacaagac tggcaacggc gttaatggtg ggtggaggaa agatatgaaa
     8821 accttcaata tatcggcctg tcatacatga tagttctact aaattaatac tcactttgag
     8881 agcgtcccgt aagggagtaa acgataagga ctagcagacg atcgtattta cctgcaatag
     8941 aattgggaac tacaacaaaa ttgcagaaaa agaatatgaa ctctcctgat gaagcatgct
     9001 cgtttgctaa aggcaaggtc gatctaacta ctctgagtaa gactgagatt atgatttatt
     9061 ataatcgtta cttattaggg ttgaataaca actgttaatc actcatatta tatggtaaaa
     9121 ttctctcgag tacagcagca tcatcagata agatcagtga aaggacggag gtattacatg
     9181 gtaagcagaa cgtaataaat acagtattac aatttacctc taatgcaaag agtagaatcg
     9241 atgcttgcgt agattatagc cggccatcat tagctgtagt acttacatag ataggtg
//
LOCUS       DAOR01000341           10576 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_23684, whole genome shotgun sequence.
ACCESSION   DAOR01000341 DAOR01000000
VERSION     DAOR01000341.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_23684"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT         3096 a         2103 c         1996 g         3381 t
ORIGIN      
        1 aacagcataa aagttgatca acagatcaat cagctaaata actgcacagg cgcaccacag
       61 gagaaggatc atatgggtag cgctacagta cctagcagta gcagtacagt atgccttaac
      121 tcaggcgaca attcagccaa catacacaaa taatcatgag tggaagtaag acgcacactc
      181 catctttttt tttgttagta caacaaaggt acgtatcccc aaaattcagc gtcgtttcac
      241 gtagctcaca ttccccgtaa tattttccat ccaacgaatt attacagtct atttatccta
      301 ggcagacttg actgatgcat gacttccacg caaatggacg tatgtatgaa gcatctgcac
      361 aggccgtagt tattaaaaga cagcatatga attgttaatc catgcataaa gtactagtca
      421 cgggcggcgc tggcttcatc ggacatcatc ttattaaaag actatctaca atggattgca
      481 aaattaccat cattgataat ctaagcaatt caaatcgcat ttttttgcat atgatcaaac
      541 gtgcaattca ggctagtagt gtaggcacat ctcgctatgt tagcttaaat gacgatggga
      601 ttgatgatgt cgcattttat gcagaagaca taagaaataa agacgctctc atagacattt
      661 tcgaaagtga agaaatagat acttgtgttc atttagcttc aaaaataagt gttccggagt
      721 cgattacaaa tcctggggac acgatagatg taaacatcaa aggaacattt aatgtcttgg
      781 aagcgtgttc aaagacggaa gtgaaaaatc tggtttttgc gtcttcctca gctgtctatg
      841 gtgaatcaaa aacgttgcct atatccgaaa aagaacaatt agatccatta tctccgtacg
      901 gtgccagtaa gatagctgga gaagctcttg tatcttcttt taggaataca cgaaaaatcc
      961 aaaatgctgt atccattcga atcttcaatg tttttgggga gggagggtac gcaggtgtaa
     1021 ttacaaagtt tgccgaacga ttattagctg gtttaccgcc agtaatctat ggtaatggaa
     1081 atcagacaag ggatttcatt tttgttgatg atgttgttag cgctatagtg ttgtcagctg
     1141 cggtcgcaga taagaaacaa gaagaaaaat cagcatccta tccttcttct tatgcgcaca
     1201 ttttaaacgt tggagccgga agagcattaa agataaaaga tctggctcaa atgatgatcc
     1261 aaatttttaa tttagatttg gagcctgttt atagcgaggc cagaagcggc gatataataa
     1321 acagtctagc cgatatgagc agattggaaa ctgttcttgg ctttgtgcct tcccgggaaa
     1381 tagtgccttc cttgaaacag atgttccccc tctgagtgaa accacttttt gcattcaaca
     1441 cattgccagg tagtcattag ccaattaacc cttcacaatc atttatctta ttcttcccgt
     1501 tatgtcgccg cactttaaat tatagtggtc taaactctgt ttgaattctg ggtttgtagc
     1561 tgcttgcgga aatttaccag cattaatgat agcatttttg ttatctagac aacctgcttt
     1621 accatcctga ttggctttat tgacgttttg tttagcaatt tggatattat tatatgacac
     1681 tgcgacagat attatcgcaa atattgccac tacgatgaat aatgcgatga cgactttaac
     1741 tactcttctc attgtttttt gacctcctcc actagcgcag tagccattat ccaaatagcc
     1801 ccatcattta accagagttc cattaattga tactgtgttt gcatgtacca gaatttgcag
     1861 atttgccagg atgtaattct gagttttcaa acgctgcgca atcacagagg tattatcctg
     1921 attaagcgct attagggtct ttagtaaatc tatcaattgt ttcaatagtt tatctttgtg
     1981 tgagcagtca attgatcact gaacccatca atctcaataa tttgagatag gccttggacc
     2041 tcctgatatc cgtagcgttg ttgattgata gctcactata gtcaaaaacc tggtataatc
     2101 atcaccattg gcatgcttat agctccgtat tcgaatttct gtataatatg cgatgtcagc
     2161 cgtgaacttt cattctcagg ttgcactctg ataggttgtt agacgtacca gctagtcctg
     2221 tccgagaatt tactcagatt taatatttcg tcgatactct tttcgcttct ggtgccgttg
     2281 tcaccatatg tcgcgaaggc aagtagcaat tgcagccttc aaggtcaagc taaccataaa
     2341 accggttccc acactctgag cagcagtgta tcttcacaga atggcagctg ttccaattct
     2401 gtggctagtc gcgcaggaca agggtctgga cataatgttt ttgcgaacgc tttttctgat
     2461 ccaggattaa gcacttctac ttgtactagc gcctcaacaa gtcacgcagg gtttcagttc
     2521 caatttaacg atggtcaaca tagcatccag gtgatagaag ttgtagcgca agctcccaat
     2581 ctccataatt ttttgcaagc gcgccatcga caactaaaat tacaccataa ttatgtttga
     2641 tgctcaagtt gccagtgcgt gactcccact tatcgcgcta gttataattc ctactttctg
     2701 tcttgtctgt tgcagcaatt tcaggaactg acgacgaata ctataaagta tattgaatat
     2761 attgacttgt gttggaaacc ataattatta atagcgttat caccattatt attatgcact
     2821 ttggagctct tagtgatctg gttaatagta ttttaacaaa gcattcgtga acattatgtc
     2881 tgctagatgg acttatgaga aattaaatgg tctcggaggg aaagaattcc aaatacttgg
     2941 ccgacaacta aatgcattct atgtcaatac catagagctc gcacaaaaag cacagggcaa
     3001 ttttgtgctt aggataaaag aaaaggaaaa caaatgcagc cgcgataacc tattgtgcta
     3061 tttagcatta aggaaacatg atttgtcaga tttacagcta agtctagctg aacaaggtct
     3121 ttcttccctg ggaatgcttg agggtgaagt actagttagt attgaacaag tcctcaaaca
     3181 ttttggaatc cgtcctggta atactagtag cttatgtaag atcaattcta aaagtgctag
     3241 tttgttattg ggaaaacgta gtaaattgat atttggctct ccgagcaaag gaagaagaac
     3301 gtgtatcatg gtaacactag actctactga tatttaccaa catgaattga ttgagcactt
     3361 acttgagagt ggtatggata tagctcgaat caattgcgca cataatacag atagggaatg
     3421 gagcttaata attaaagcta tacacgaagc tgaagaacaa ttagttcagc gtgagaaagg
     3481 gactaggagc agatgtatga tcttaatgga tctaggaggt cccaagatta ggacgggtca
     3541 tatggagttc aaagtgcgtc cacttaaaat tatcgttcca aaagataagt atggtcggcc
     3601 tgtgcgcttt gttgaaggat tcttagatac cgaagcaagc tacacagaac tagtcgacca
     3661 agtaacaggg ccatcgactt ttgttatagc gatatcaaca ggcaacgatc gtgggttagg
     3721 tagtttacag aaagaccaaa agataacttt caaggacgca cgtgatgagc accaacgtac
     3781 aatcactgta cttgaacgaa caagtcctac tcgggtgcga ataggactgg aacaaactgc
     3841 atatctaaaa gagggtataa aattagaatg tcaaacaaat agtaatagta ataaaaagtg
     3901 ttcattcaca gtcggagcta ttaaaccaca gccacttgag ttaaaagtag aggcgggtga
     3961 tgtattgcgt ctttataggg atgcaaggct aggtcattct ggtcctggat ctggatctga
     4021 tggcaagcct gctgcagcta ttagttgtac atatccagaa gttcttgacc aggtcaaaat
     4081 tggccacaga atattcatag acgacggaaa aatcgaagca gttgtgcgtt catcaaatga
     4141 acaatatctc ggattagaga tcatttcgcc caaaggaata atggccaaga tcaaatccaa
     4201 taagggtata aactttccag attcatctct taaaatgccc gccctgacac ctgacgacat
     4261 caggaatctg ggttttgtcg tggaacacgc agatatggta ggattaagtt tcgttcatag
     4321 accagaggat ctttacgatt tacgcgaaat attgtctaaa ttaggtcatc caaatttggg
     4381 tatagttgca aagatagaaa cagcggattc aatccacaat ctggctaaga tacttattgc
     4441 aggtcttgaa ttaccaaaat ttgcaatatt gatcgcaagg ggtgatttag ctgttgaggt
     4501 aggatttgag aatttggcat tcgttcaaga agacatactc tgtttatgtg aagcagcaca
     4561 tatccctgtc atactggcca cacaaatact tgaaagcttg gctgaaagtg gtcttcgaag
     4621 tcgcgcagaa atgacagatg caattatggg acaacgtgct gaatgcgtaa tgttaaacaa
     4681 tggcatacat atacttgaag cagtcagaac cttagcaata ttattaagta ctgaagaacg
     4741 acatcaagtg aagaaacatc aactgttcag tgaatttaca tttcagcatg ctgtgtttga
     4801 ggaagagaat tgacaattaa aatgaaattc agacatgatc ggcattgatt tgaaagaatt
     4861 ccatgacttt caggcttgtt aggtactgct tagtctagaa gtctgcatcg tccatccatg
     4921 tattacttca ttcttcttac agaccccgtt agatctaagt tctttactct gtctggtctt
     4981 ctaaaatcat tactgatctc attctacaag cagtttatgt gatatgtcct ttactactat
     5041 tgattttacg tttacaaatt ctcgtgtacc gtagtgtgat aattctctac ctattccaga
     5101 tgattttata cccccaaaag gcaatctggg atctgatctt accatttcat ttactctgac
     5161 cactccgctt cgaatgtctc gtgcaagtcg cacacctttc tcaaagttac tcgtccatat
     5221 actcgcaccc aatccaaatt ctgaattgtt agcttcccat atagcgtcgg cctcgttatc
     5281 cactacgata ataggtgccg caggtccaaa tacctcttca tttaaaacaa ccatttgatg
     5341 atttacgtta gttatgatcg ttggctcgta aaagtatcca tcaccagcta ttatcctgcc
     5401 gcctgtcaat attttaccac ctttcgactt tgcgtcttct acttgattta ttagagtttg
     5461 tcgttggctt tccctaacta atggtccaac tgtggttttg gaattcatgg gatctccaac
     5521 gacttctgac tctacattgt gtgtaaatag ttttgaaaat ttctccgcca cttcctttac
     5581 aacgatgaat ctctttgctg caatacagct ctggccagtg ttaagtaatc tggattgggc
     5641 tgccaggtgt gctgtttgat ttacgtcagc gtcttccagc actacgaaag gatcactccc
     5701 tcctaattct aaaacacact ttttcaatcc tcttgcagca agctccgcaa ccctttttcc
     5761 agtattaaca ctccctgtta ctgaaactgc atcaatctta tctgattgta ctagtgcctc
     5821 tccagccttg tagtcaccta taatacactg aaagacattc tcaggaaatc cactttctct
     5881 aaaagcttct tgaatttgta aagcagatcc tgtacataca cttgagtgct taagtacaac
     5941 tacattgcca gcaattaatg tcggaatagc ataacgcatg acttgccaaa atggaaaatt
     6001 ccacggcata atacctgcga ctactcctag aggctcaaat gaaatgaagc tcttgcggaa
     6061 ttcggtcgga actatttcgt cacgcaagaa tacttctgca ttttcagaat aatattcaca
     6121 taccaaaaca catttctcta tttctgcaat tgcctgagtt atgggtttcc ccatttcttc
     6181 tgtaacgatt tttgcatatc tgttcttatt cttagtgagt attttggcca ggtctcgaat
     6241 gtaatcacag cgctccaaaa tttccttctt cctccatttc tcaaaggcat tttgtgcatt
     6301 ttttgctata tgttcaatct gttctattga catgatgtca taggacaaaa taacctttcc
     6361 agttgtagga ttaaccgttt ggattttagc attattagcc atgctcatgt atcacgagct
     6421 aaataatacg catttgcgct taataaagtc aatagtctat ttaggggata aattatgtgt
     6481 gatacttatt caaaccaata agtaagggtt tcttgccctt tttttgttct actcctagtc
     6541 tgcagatgat tgcggcaagg aaaataaaat aaaatcatgg attaaaccat acaatcaagc
     6601 tctgaaagtg aaacctgcaa atcattacat ttcgtaacca ctagatcctc tgaatttcaa
     6661 ttattttctt ttttgatgtt aatccagaaa tgatacgtat gctattttcg ggtactccaa
     6721 agaaatctga tgcttttctt atcagctcct tgtttgcttt tccatgtttt ggttcagatt
     6781 ttatagccat tattatttca tccccattta cttgaatttg tcctgatgaa ttgaatttga
     6841 cttcgacgaa atacctcaat taattaactc aatgcataat aattcatgat ttttatttat
     6901 ctattagggt gtctctaaaa cagacgaatc tgggagcgtt tagagacttt ttttgaattg
     6961 gaatccgact gtaaatacta ttctcttcat tggttgaagt tcatgaatgc tcagattttt
     7021 caaattccaa accttgttca gacgcaaagg gtaatgtatt tcctgtcctg ccgtatttgc
     7081 ctgcatgtag tttgatagtt gatgtggatg cataccattt gccgttatca acaaaatcaa
     7141 accaaagagg ccgtcttatt tcttctgatt tgctttattt tattttatta ttcagatgat
     7201 ttcacttact gaaatttcca gcctagaacc tgttaaaatt agatccatac atactgtctg
     7261 tcgtactact cttagaaaaa aaagagaggg aacgtgtctt gctttcattc aagattattt
     7321 gtgtatgtcg gctgaattgt cggctgagtt aaggcatact gtgccgctaa cgctaccgcc
     7381 atcgctaacg ctaccgccag tcaaaccgag ggtactgacg cctagagtgg aactttcggg
     7441 ttgcgggact ggtacgccag tacagctatt ttgctgatta acctgttgat caacttttat
     7501 gctgtttttg tgattgttgt tattgttgtg gtgtttgtac gccaacgcct gttgtggtat
     7561 agcgaatgct actgccgtca gtgtcatagc tgcgacgaca gctacgattg ctaaagctgc
     7621 attgtggttc atttctcaga gggctatcta caagtattct atatttgaga tgctccacag
     7681 caactgctag aaatagagaa gaatttgtcg atatattcaa accctgatat attcgattag
     7741 aaagattata gtcataatct tgctttatct attgaagaga atacaaccat ggttaatatc
     7801 tttcatcgat tatgaataat actcgaataa cgaccaatcc gatttattcg agtatttaac
     7861 aataaggatt ttatagtttg gcttgaagaa gacgtagagt agttgttaga gtattgggtg
     7921 gctgatctac tcttccaact ccaagtctct aagccgtcta cactctttta gacttgatca
     7981 ataacaacat atagacaatt tgatactatt agaataagtt tgtattatta tttctattga
     8041 gattagctcc atgcccaccg ttgtattgac aaaaatggag agtaagttcg agattatttg
     8101 cgtatgtcgg cactattttg ggctctactg tcgccgaagc tctgaaaatc tgtggatggc
     8161 tggagccagc gatagcggat gggggcttga gcagcatctg ctgattgaca gtcattagca
     8221 tctactcgaa cctcgaagct gatcagatat tgcagcaata tcccaaatac gctttttatt
     8281 atttttgatg ctgttgatag tggaggaatt tgatgttgat gctgataaca atattccatc
     8341 ctctatgagt tcatttacaa tcatagctgc cataggtaaa gctccagtcg cgcctggaga
     8401 gttataatta agcacgtgta gagtattttc atttttaata atcagcgtgt cttcaacaaa
     8461 ttttccgttt ctatctatca aagaggatct gatgcctgcg gtccctcttt gtgtaaaaga
     8521 cgatggccta agttggggta ggaattctct tgccctattt atcatggcag ttttagaaag
     8581 tgagcttttt aattcactac ttgccagcaa tagaaattgt ctattgagga atattttccg
     8641 tgctccggtt ttggatgatt caaaaatctt tggtatcaaa tacttgacgt tagtgttcca
     8701 actatacgca taaggcgaaa agacagggac agcattaggg cctacctccc gcttgccgtc
     8761 aacgcgcagt atccaatggg ggtccaaaaa aggatattct ggatgttttg gaactgtgta
     8821 gatacttact ttcgtcagat cacgatattc cgagggcgcc tgccaatatt cccctctgaa
     8881 atgaagatcg gtatattcgc gagctactcc cataccgtgt gctatgtcaa ctgcatttcc
     8941 gccagctgtg ttgatgagat atttggttac aaacttctct ccattgtttg tgtaaattgc
     9001 tatatgacca tcatcgttgt tgtggttgtg atttcttcgt attatcctaa caactttact
     9061 acctgtaact agcttgcatc caaaaagcct tgcatcttcc ataagttccc tagttaccgc
     9121 cccataatcg atagaaccat ccttagagca ataaattcct gctaaacatt ttacattagg
     9181 ttcgaaatca tgtacctcgt ccttttctaa gaactgaatt tcattttttt gtaatccatt
     9241 tgagtaaccc cattctaagt atttgtgaag cctatctatt cccttatcgt aggttgcaac
     9301 ttcaaggact ccatcttgtt taaaagataa cgacttcaaa ctgcaatatt cttttaacat
     9361 gctaaaacca agaaatgcgg cttttgcaac gagctttttc ctgaccgggt cgtatagaaa
     9421 tggcgcatgg actttaccag tatttctggc actggcatgc aaagaaatgt ccgcttcctg
     9481 ttcaataaga agtatattat tagagctttg tgcagctgag gagcataaaa aatatgcgat
     9541 ggaggtgcct aaaataccac ctccaataat tgcaatatca aatcgatttt ctgaattcaa
     9601 tgagcatgtt caacaaataa ttattgtagt tatgtcatat tattagtttc ttcgccatca
     9661 ttgctataat gactaactaa ttccttttta tttttgtata gctatattat cttatactag
     9721 ttcacgctct ccataatttt tattatttat attggctcta tttacaagcg gataaacgct
     9781 tcaaccaggc cacctacaac aagtgtttac caagaagcac cgcttacaaa acatattagc
     9841 cgtcctaata ccaaattaca tgatttctct tagaacaaaa attaaatttt ttcttcgtta
     9901 atttgtttta ttttttctag aacaagatcg ctcagctctt taatcattgg aacattcgtt
     9961 agctcgacaa gtgctggtaa tgcacttgtc ttgtaagagg ccaatgcctc gatcaatttt
    10021 tttcttctat caatattcca agaccaatat ataagaatct taagattttc gatctcccat
    10081 ttctcctgcc actcctacaa ttgtgtagac ttcctcaatg ccactatgtg ttttgttatt
    10141 attataccaa tctcggaaag cttgagcgtc tttttcatcc aattttacta ttctttctac
    10201 attttgagct gcgtttagat tctggggtac aagattctct aattctacta gtgcgagact
    10261 atgtaccata gacattgaca taaaagtcgt ttcttagctt taacccttcg tccgacgaaa
    10321 ttatacattc acagaaaatt gcattaatcc gacgttaagg atacagaatc cattttaggg
    10381 ctctgttaac ataaaagatg gtttaagaca taacaaacgc ggacatattc aaaaatacga
    10441 atgcagagat tgcagacggt atttcacaat caatttggga tttgaaagaa cgcgtgccac
    10501 tccccaaatc ataacgtctg cgctgcaatt gtattttact ggagagtcat tgcgcaatgt
    10561 tcaaaaattc ttgaaa
//
LOCUS       DAOR01000342            3065 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris nunavutensis isolate UBA273
            UBA273_contig_56639, whole genome shotgun sequence.
ACCESSION   DAOR01000342 DAOR01000000
VERSION     DAOR01000342.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027531
            Sequence Read Archive: SRR5037199
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris nunavutensis (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris nunavutensis
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 14.38x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Nitrosopolaris nunavutensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA273_contig_56639"
                     /isolate="UBA273"
                     /isolation_source="soil"
                     /db_xref="taxon:2959593"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /metagenome_source="soil metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX710580;
                     metagenomic"
BASE COUNT          870 a          636 c          560 g          999 t
ORIGIN      
        1 tatgtgtgta agtacaacac ttagttactt tgcatgttgt atataataca caagaaagat
       61 tccaaaaagc attagaagac tagtaactag agaactaaat cagttagata tggggacttg
      121 ttaaatatag ttaattatct accaagtccg tgatatccgt cgaatcgtcc caaatccgaa
      181 tatctagcat ctccgataat cccccagaag caattctcac attgtcctac tatatggtca
      241 ggaccgcaga accaggccct acctttgcac ttatcggcta acttattgag gtcactctgg
      301 tctttatacg actctggtat cgcatgtaaa tcagaattga cggtcccgac actgcaatcc
      361 atcagctcgg ctatttgctt atgactcatt ttttgctccg tgtacagctt gtatatcttg
      421 tattccctgt attgtttagg gttcaaggct gcctcttttg ttcgttcagg ttcaacctag
      481 acaacaccag ctagataatc agggctgcaa gaccacaacg acgaatacca aaaagactca
      541 ctgttgaata tatgggagaa caagaataaa tggcgctagc agtgatagat agactatgta
      601 tggaaaagac tttgatatcg gtcaaagagt tcccagttcc tgaagtaggc agacgtcaga
      661 aagataggcg taagagatgt gtgttttgtg gcaatttagc ctcacatgaa gctatcttta
      721 gagtgcaagg ggcgtcactt gtcgaatggt attgcggcgc acatgtcaaa cggttaaagt
      781 aagtaatatt atagtgactg tgttatttta tccatctgac gaatccattc cacatatttt
      841 tgttgaaggt cacttaattt cttttttgat ttagatttag attgcatttt gttctgtaaa
      901 acgtcggcaa ttgcatactt tagaattaaa gtgtctccat gtgcacttgt ttttgtgtaa
      961 tgattctaca tgattgcatt tgcaacagcg tcgaatcaat attctttctt agtcctaacg
     1021 tatggaattg ttagccttac tccatcaaat aatctaaagg tggacagatg ttcatttttt
     1081 agtgtggatt tggcagatgt tatacttact taagattgga agtaatcaac aaaactttgg
     1141 actttaatct gcaatgttaa ctctagctgt gttaatagct catcttcgat atcttccctt
     1201 tttagccaat ctattgtatg tgggattcga acaggttgag acagttaacg atcctatatt
     1261 cgctctgaag aacttcaagg caggttcgta tgatctgcta attgtagaca ttgtaatgcc
     1321 agaaatggat ggattcagcc tatacgaagg aattagaaaa atagacaaca aagttaaggt
     1381 ttgttttatc accgcatttg agatgaatta tcaagctctg agagcagtat tttctgctgc
     1441 cactgctact gatgatatcg gatgcttcat ccgaaaacca gttgatatgc atgatttggt
     1501 aaaaataata aatgcggaga tacagttggc aaacgacaga acctacgagt catgcggggt
     1561 ccaaacgggc cgcttcagcc ggtgcgtatt gctgtcaact actcccgggg cttcctgcat
     1621 cctcacctct ctggaagtac cgcaggcttt tggccgtttc aagtcacgag cgcggccgat
     1681 gccgggcgtc agaaaggcgt cctcgctgcc attgtcgagt tagaactgca tcagcgcatc
     1741 tttgaaaccg attggcgtat tctgcccaga gaagaagagc atcagaaata attcgacaga
     1801 ttgatatcgt ttctcaagta atagttatta ccaaacttta ttatcaatct gagctaatca
     1861 ttagttgttg ctgttgtttt gctacctctt actaccaaca ttcttcattg atactccttc
     1921 ttcctcgata tcgtcagcgc ctttttcatt tattgtaaat ctagtatcag aatacggatg
     1981 atatggcgag tccatcatct ttgctaccct gttctctcca gactttctaa gatatatcct
     2041 ataagttgaa gagtgtgcta ctatattgcc accagctgct tttgtaggat ctccgaagaa
     2101 ggtatctgga gaggattgaa cttgatttgt tataacgaca gcaatgatga atatctctgc
     2161 taaccttata atcagatgca acagtatggg ctagagatct tgattcttat attgtaaact
     2221 tagatgaaac aattggagat atattagtaa atccaaatgg cacggaggac taacctttta
     2281 ttttataccc tatcttcttc ttgagtaggt caagcgttta ggctacttcc aagaggatcc
     2341 tcccatctct ttccttatca aaaagtgcct gtaaggcttc tttagcttca tcaagcgtga
     2401 actttttcca cactctagtc tttaattctt tagacatatc tatgagttct gtgatctctt
     2461 ttctagtgcc accagtagaa cctattaatt ttacttgctt agaataaagt gattgaatat
     2521 ttaatttaac atctgcacct gttagacctc caaatgttac caatctccca ttaaggccaa
     2581 ctgatgcaaa gctattttcc catgtttgta cccctaaaga atttaaaaca acatctgcca
     2641 ttttgccttg agttaattca ttaattttct ctaccactct atcatattca cctataatgt
     2701 aatcggctcc aaaatctttt acccaatcat ctttagagac cgcaataacc tttgctccca
     2761 ttttctttcc aaattgtata gccatcatcc ctgtatttcc tgatgcacca aatattacca
     2821 aggattcatt tacttttaat gctgcttctt ttaatgcatg ataaggtgtt aaagtggtta
     2881 caggtagact ggccgctaac tcccattgca tttcgtctgg tattttaaag acatttttct
     2941 ccggaacagt gatgtattct gcaaagcctc cattagtagc aacacctatc aatcctccgt
     3001 ttctacataa catatcattt ccactcagac acatatcaca aattccatca aacaccttgt
     3061 tatgc
//
